TJP1 gene cDNA ORF clone, Pan troglodytes(chimpanzee)

mRNA	Protein	Name
XM_016927842.1	XP_016783331.1	tight junction protein ZO-1 isoform X12
XM_009428684.2	XP_009426959.2	tight junction protein ZO-1 isoform X1
XM_009428686.2	XP_009426961.2	tight junction protein ZO-1 isoform X3
XM_009428689.2	XP_009426964.2	tight junction protein ZO-1 isoform X4
XM_009428687.2	XP_009426962.2	tight junction protein ZO-1 isoform X5
XM_009428688.3	XP_009426963.2	tight junction protein ZO-1 isoform X6
XM_024349007.1	XP_024204775.1	tight junction protein ZO-1 isoform X9
XM_009428690.2	XP_009426965.2	tight junction protein ZO-1 isoform X9
XM_016927840.1	XP_016783329.1	tight junction protein ZO-1 isoform X8
XM_016927841.1	XP_016783330.1	tight junction protein ZO-1 isoform X11
XM_009428684.3	XP_009426959.2	tight junction protein ZO-1 isoform X1
XM_024349008.1	XP_024204776.1	tight junction protein ZO-1 isoform X10
XM_016927842.2	XP_016783331.1	tight junction protein ZO-1 isoform X13
XM_001163045.4	XP_001163045.2	tight junction protein ZO-1 isoform X15
XM_016927839.1	XP_016783328.1	tight junction protein ZO-1 isoform X7
XM_001163120.3	XP_001163120.2	tight junction protein ZO-1 isoform X10
XM_009428685.3	XP_009426960.2	tight junction protein ZO-1 isoform X2
XM_009428689.3	XP_009426964.2	tight junction protein ZO-1 isoform X4
XM_009428687.3	XP_009426962.2	tight junction protein ZO-1 isoform X5
XM_024349006.1	XP_024204774.1	tight junction protein ZO-1 isoform X7
XM_009428690.3	XP_009426965.2	tight junction protein ZO-1 isoform X8
XM_016927841.2	XP_016783330.1	tight junction protein ZO-1 isoform X12
XM_009428685.2	XP_009426960.2	tight junction protein ZO-1 isoform X2
XM_009428688.2	XP_009426963.2	tight junction protein ZO-1 isoform X6
XM_001163045.3	XP_001163045.2	tight junction protein ZO-1 isoform X13
XM_016927843.1	XP_016783332.1	tight junction protein ZO-1 isoform X14
XM_009428686.3	XP_009426961.2	tight junction protein ZO-1 isoform X3
XM_001163120.4	XP_001163120.2	tight junction protein ZO-1 isoform X11
XM_024349009.1	XP_024204777.1	tight junction protein ZO-1 isoform X14
XM_016927843.2	XP_016783332.1	tight junction protein ZO-1 isoform X16

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPc69378	XM_009428687.2 Latest version!	Pan troglodytes tight junction protein 1 (TJP1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OPc69379	XM_009428688.2 Latest version!	Pan troglodytes tight junction protein 1 (TJP1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OPc69380	XM_016927839.1 Latest version!	Pan troglodytes tight junction protein 1 (TJP1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OPc69381	XM_016927840.1 Latest version!	Pan troglodytes tight junction protein 1 (TJP1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OPc69383	XM_001163120.3 Latest version!	Pan troglodytes tight junction protein 1 (TJP1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OPc69384	XM_016927841.1 Latest version!	Pan troglodytes tight junction protein 1 (TJP1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OPc69385	XM_016927842.1 Latest version!	Pan troglodytes tight junction protein 1 (TJP1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OPc69386	XM_001163045.3 Latest version!	Pan troglodytes tight junction protein 1 (TJP1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OPc69387	XM_016927843.1 Latest version!	Pan troglodytes tight junction protein 1 (TJP1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPc97826	XM_009428684.3 Latest version!	Pan troglodytes tight junction protein 1 (TJP1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPc97827	XM_009428685.3 Latest version!	Pan troglodytes tight junction protein 1 (TJP1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPc97828	XM_009428686.3 Latest version!	Pan troglodytes tight junction protein 1 (TJP1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPc97829	XM_009428689.3 Latest version!	Pan troglodytes tight junction protein 1 (TJP1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPc97830	XM_009428687.3 Latest version!	Pan troglodytes tight junction protein 1 (TJP1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPc97831	XM_009428688.3 Latest version!	Pan troglodytes tight junction protein 1 (TJP1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPc97832	XM_024349006.1 Latest version!	Pan troglodytes tight junction protein 1 (TJP1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPc97833	XM_009428690.3 Latest version!	Pan troglodytes tight junction protein 1 (TJP1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPc97834	XM_024349007.1 Latest version!	Pan troglodytes tight junction protein 1 (TJP1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPc97835	XM_024349008.1 Latest version!	Pan troglodytes tight junction protein 1 (TJP1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPc97836	XM_001163120.4 Latest version!	Pan troglodytes tight junction protein 1 (TJP1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPc97837	XM_016927841.2 Latest version!	Pan troglodytes tight junction protein 1 (TJP1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPc97838	XM_016927842.2 Latest version!	Pan troglodytes tight junction protein 1 (TJP1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPc97839	XM_024349009.1 Latest version!	Pan troglodytes tight junction protein 1 (TJP1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPc97840	XM_001163045.4 Latest version!	Pan troglodytes tight junction protein 1 (TJP1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPc97841	XM_016927843.2 Latest version!	Pan troglodytes tight junction protein 1 (TJP1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OPc69374	XM_009428684.2 Latest version!	Pan troglodytes tight junction protein 1 (TJP1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OPc69375	XM_009428685.2 Latest version!	Pan troglodytes tight junction protein 1 (TJP1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OPc69376	XM_009428686.2 Latest version!	Pan troglodytes tight junction protein 1 (TJP1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OPc69377	XM_009428689.2 Latest version!	Pan troglodytes tight junction protein 1 (TJP1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OPc69382	XM_009428690.2 Latest version!	Pan troglodytes tight junction protein 1 (TJP1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00

TJP1 ( XM_009428687.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009426962.2 Pan troglodytes tight junction protein 1 (TJP1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OPc69378
Clone ID Related Accession (Same CDS sequence)	XM_009428687.2
Accession Version	XM_009428687.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5319bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	tight junction protein ZO-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006482.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009428687.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGAA ATACGTTTTC AATGGATTGC CACAGTAAGA GCACAGCAAT GGAGGAAACA 
GCTATATGGG AACAACATAC AGTGACGCTT CACAGGGCTC CTGGATTTGG GTTTGGAATT 
GCAATATCTG GTGGACGAGA TAATCCTCAT TTTCAGAGTG GGGAAACGTC AATAGTGATT 
TCAGATGTGC TGAAAGGAGG ACCAGCTGAA GGACAGCTAC AGGAAAATGA CCGAGTTGCA 
ATGGTTAACG GAGTTTCAAT GGATAATGTT GAACATGCTT TTGCTGTTCA GCAACTAAGG 
AAAAGTGGGA AAAATGCAAA AATTACAATT AGAAGGAAGA AGAAAGTTCA AATACCAGTA 
AGTCGTCCTG ATCCTGAACC AGTATCTGAT AATGAAGAAG ATAGTTATGA TGAGGAAATA 
CATGATCCAA GAAGTGGCCG GAGTGGTGTG GTTAACAGAA GGAGTGAGAA GATTTGGCCG 
AGGGATAGAA GTGCAAGTAG AGAGAGGAGC TTGTCCCCGC GGTCAGACAG GCGGTCAGTG 
GCTTCCAGCC AGCCCGCTAA ACCTACTAAA GTCACACTGG TGAAATCCCG GAAAAATGAA 
GAATATGGTC TTCGATTGGC AAGCCATATA TTTGTTAAGG AAATTTCACA AGATAGTTTG 
GCAGCAAGAG ATGGCAATAT TCAAGAAGGT GATGTTGTAT TGAAGATAAA TGGTACTGTG 
ACAGAAAATA TGTCATTGAC AGATGCAAAG ACATTGATAG AAAGGTCTAA AGGCAAATTA 
AAAATGGTAG TTCAAAGAGA TGAACGGGCT ACGCTATTGA ATGTCCCTGA TCTTTCTGAC 
AGCATCCACT CTGCTAATGC CTCTGAGAGA GACGACATTT CAGAAATTCA GTCACTGGCA 
TCAGATCATT CTGGTCGATC ACACGATAGG CCTCCCCGCC GCAGCCGGTC ACGATCTCCT 
GACCAGCGGT CAGAGCCTTC TGATCATTCC AGGCACTCGC CACAGCAGCC AAGCAATGGC 
AGTCTCCGGA GTAGAGATGA AGAGAGAATT TCTAAACCTG GGGCTGTCTC AACTCCTGTA 
AAGCATGCTG ATGATCACAC ACCTAAAACA GTGGAAGAAG TTACAGTTGA AAGAAATGAG 
AAACAAACAC CTTCTCTTCC AGAACCAAAG CCTGTGTATG CCCAAGTTGG GCAGCCAGAT 
GTGGATTTAC CTGTCAGTCC ATCTGATGGT GTCCTACCTA ATTCAACTCA TGAAGATGGG 
ATTCTTCGGC CCAGCATGAA ATTGGTAAAA TTCAGAAAAG GAGATAGTGT GGGTTTGCGG 
CTGGCTGGTG GAAATGATGT TGGAATATTT GTAGCTGGCG TTCTAGAAGA TAGCCCTGCA 
GCCAAGGAAG GCTTAGAGGA AGGTGATCAA ATTCTCAGGG TAAACAACGT AGATTTTACA 
AATATCATAA GAGAAGAAGC CGTCCTTTTC CTGCTTGACC TCCCTAAAGG AGAAGAAGTG 
ACCATATTGG CTCAGAAGAA GAAGGATGTT TATCGTCGCA TTGTAGAATC AGATGTAGGA 
GATTCTTTCT ATATTAGAAC CCATTTTGAA TATGAAAAGG AATCTCCCTA TGGACTTAGT 
TTTAACAAAG GAGAGGTGTT CCGTGTTGTG GATACCTTGT ACAATGGAAA ACTGGGCTCT 
TGGCTTGCTA TTCGAATTGG TAAAAATCAT AAGGAGGTAG AACGAGGCAT CATCCCTAAT 
AAGAACAGAG CTGAGCAGCT AGCCAGTGTA CAGTATACAC TTCCAAAAAC AGCAGGCGGA 
GACCGTGCTG ACTTCTGGAG ATTCAGAGGT CTTCGCAGCT CCAAGAGAAA TCTTCGAAAA 
AGCAGAGAGG ATTTGTCCGC TCAGCCTGTT CAAACAAAGT TTCCAGCTTA TGAAAGAGTG 
GTTCTTCGAG AAGCTGGATT TCTGAGGCCT GTAACCATTT TTGGACCAAT AGCTGATGTT 
GCCAGAGAAA AGCTGGCAAG AGAAGAACCA GATATTTATC AAATTGCAAA GAGTGAACCA 
CGAGACGCTG GAACTGACCA ACGTAGCTCT GGCATTATTC GCCTGCATAC AATAAAGCAA 
ATCATAGATC AAGACAAACA TGCTTTATTA GATGTAACAC CAAATGCAGT TGATCGTCTT 
AACTATGCCC AGTGGTATCC AATTGTTGTA TTTCTTAACC CTGATTCTAA GCAAGGAGTA 
AAAACAATGA GAATGAGGTT ATGTCCAGAA TCTCGGAAAA GTGCCAGGAA GTTATATGAG 
CGATCTCATA AACTTCGTAA AAATAATCAC CATCTTTTTA CAACTACAAT TAACTTAAAT 
TCAATGAATG ATGGTTGGTA TGGTGCGCTG AAAGAAGCAA TTCAACAACA GCAAAACCAG 
CTGGTATGGG TTTCCGAGGG AAAGGCGGAT GGTGCTACAA GTGATGACCT TGATTTGCAT 
GATGATCGTC TGTCCTACCT GTCAGCTCCA GGTAGTGAAT ACTCAATGTA TAGCACGGAC 
AGTAGACACA CTTCTGACTA TGAAGACACA GACACAGAAG GCGGGGCCTA CACTGATCAA 
GAACTAGATG AAACTCTTAA TGATGAGGTT GGGACTCCAC CGGAGTCTGC CATTACACGG 
TCCTCTGAGC CTGTAAGAGA GGACTCCTCT GGAATGCATC ATGAAAACCA AACATATCCT 
CCTTACTCAC CACAAGCGCA GCCACAACCA ATTCATAGAA TAGACTCCCC TGGATTTAAG 
CCAGCCTCTC AACAGAAAGC AGAAGCTTCA TCTCCAGTCC CTTACCTTTC GCCTGAAACA 
AACCCAGCAT CATCAACCTC TGCTGTTAAT CATAATGTAA ATTTAACTAA TGTCAGACTG 
GAGGAGCCCA CCCCAGCTCC TTCCACCTCT TACTCACCAC AAGCTGATTC TTTAAGAACA 
CCAAGTACTG AGGCAGCTCA CATAATGCTA AGAGATCAAG AACCATCATT GTCGTCGCAT 
GTAGATCCAA CAAAGGTGTA TAGAAAGGAT CCATATCCCG AGGAAATGAT GAGGCAGAAC 
CATGTTTTGA AACAGCCAGC CGTTAGTCAC CCAGGGCACA GGCCAGACAA AGAGCCTAAT 
CTGACCTATG AACCCCAACT CCCATACGTA GAGAAACAAG CCAGCAGAGA CCTCGAGCAG 
CCCACATACA GATACGAGTC CTCAAGCTAT ACGGACCAGT TTTCTCGAAA CTATGAACAT 
CGTCTGCGAT ACGAAGATCG CGTCCCCATG TATGAAGAAC AGTGGTCATA TTATGATGAC 
AAACAGCCCT ACCCATCTCG GCCACCTTTT GATAATCAGC ACTCTCAAGA CCTTGACTCC 
AGACAGCATC CCGAAGAGTC CTCAGAACGA GGGTACTTTC CACGTTTTGA AGAGCCAGCC 
CCTCTGTCTT ACGACAGCAG ACCACGTTAC GAACAGGCAC CTAGAGCATC CGCCCTGCGG 
CACGAAGAGC AGCCAGCTCC TGGGTATGAC ACACATGGTA GACTCAGACC GGAAGCCCAG 
CCCCACCCTT CAGCAGGGCC CAAGCCCGCA GAGTCCAAGC AGTATTTTGA GCAATATTCA 
CGCAGTTACG AGCAAGTACC ACCCCAAGGA TTTACCTCTA GAGCAGGTCA TTTTGAGCCT 
CTCCATGGTG CTGCAGCTGT CCCTCCGCTG ATACCTTCAT CTCAGCATAA GCCAGAAGCT 
CTGCCTTCAA ACACCAAACC ACTGCCTCCA CCCCCAACTC AAACCGAAGA AGAGGAAGAT 
CCAGCAATGA AGCCACAGTC TGTACTCACC AGAGTTAAGA TGTTTGAAAA CAAAAGATCT 
GCATCCTTAG AGACCAAGAA GGATGTAAAT GACACTGGCA GTTTTAAGCC TCCAGAAGTA 
GCATCTAAAC CTTCAGGTGC TCCCATCATT GGTCCCAAAC CCACTTCTCA GAATCAGTTC 
AGTGAACATG ACAAAACTCT GTACAGGATC CCAGAACCTC AAAAACCTCA ACTGAAGCCA 
CCTGAAGATA TCGTTCGGTC CAATCATTAT GACCCTGAAG AAGATGAAGA ATATTATCGA 
AAACAGCTGT CATACTTTGA CCGAAGAAGT TTTGAGAATA AGCCTCCTGC ACACATTGCC 
GCCAGCCATC TCTCCGAGCC TGCAAAGCCA GCTCATTCTC AGAATCAATC AAATTTTTCT 
AGTTATTCTT CAAAGGGAAA GCCTCCTGAA GCTGATGGTG TGGATAGATC ATTTGGCGAG 
AAACGCTATG AACCCATCCA GGCCACTCCC CCTCCTCCTC CATTGCCCTC GCAGTATGCC 
CAGCCATCTC AGCCTGTCAC CAGCGCGTCT CTCCACATAC ATTCTAAGGG AGCACATGGT 
GAAGGTAATT CAGTGTCATT GGATTTTCAG AATTCCTTAG TGTCCAAACC AGACCCACCT 
CCATCTCAGA ATAAGCCAGC AACTTTCAGA CCACCAAACC GAGAAGATAC TGCTCAGCCA 
GCTTTCTATC CCCAGAAAAG TTTTCCAGAT AAAGCCCCAG TTAATGGAAC TGAACAGACT 
CAGAAAACAG TCACTCCAGC ATACAATCGA TTCACACCAA AACCATATAC AAGTTCTGCC 
CGACCATTTG AACGCAAGTT TGAAAGTCCT AAATTCAATC ACAATCTTCT GCCAAGTGAA 
ACTGCACATA AACCTGAATT GTCTTCAAAA ACTCCCACTT CTCCAAAAAC TCTTGTGAAA 
TCGCACAGTT TGGCACAGCC TCCTGAGTTT GACAGTGGAG TTGAAACTTT CTCTATCCAT 
GCAGAGAAGC CTAAATATCA AATAAATAAT ATCAGCACAG TGCCTAAAGC TATTCCTGTG 
AGTCCTTCAG CTGTGGAAGA GGATGAAGAT GAAGATGGTC ATACTGTGGT GGCCACAGCC 
CGAGGCATAT TTAACAGCAA TGGGGGCGTG CTGAGTTCCA TAGAAACTGG CGTTAGTATA 
ATTATCCCTC AAGGAGCCAT TCCCGAAGGA GTTGAGCAGG AAATCTATTT CAAGGTCTGC 
CGGGACAACA GCATCCTTCC ACCTTTAGAT AAAGAGAAAG GTGAAACACT GCTGAGTCCT 
TTGGTGATGT GTGGTCCCCA TGGCCTCAAG TTCCTGAAGC CTGTGGAGCT GCGCTTACCA 
CACTGTGCGT CCATGACTCC TGACGGTTGG TCTTTTGCTC TAAAATCATC CGACTCCTCG 
TCGGGTGATC CTAAAACCTG GCAAAACAAG TGTCTTCCCG GAGATCCAAA TTATCTCGTT 
GGAGCAAACT GTGTTTCTGT CCTTATTGAC CACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009426962.2
CDS	110..5428
Translation	MERNTFSMDCHSKSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQKAEASSPVPYLSPETNPASSTSAVNHNVNLTNVRLEEPTPAPSTSYSPQADSLRTPSTEAAHIMLRDQEPSLSSHVDPTKVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQPAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPELSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_009428687.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes tight junction protein 1 (TJP1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009428687.2

ORF Insert Sequence:

ATGGAGAGAA ATACGTTTTC AATGGATTGC CACAGTAAGA GCACAGCAAT GGAGGAAACA 
GCTATATGGG AACAACATAC AGTGACGCTT CACAGGGCTC CTGGATTTGG GTTTGGAATT 
GCAATATCTG GTGGACGAGA TAATCCTCAT TTTCAGAGTG GGGAAACGTC AATAGTGATT 
TCAGATGTGC TGAAAGGAGG ACCAGCTGAA GGACAGCTAC AGGAAAATGA CCGAGTTGCA 
ATGGTTAACG GAGTTTCAAT GGATAATGTT GAACATGCTT TTGCTGTTCA GCAACTAAGG 
AAAAGTGGGA AAAATGCAAA AATTACAATT AGAAGGAAGA AGAAAGTTCA AATACCAGTA 
AGTCGTCCTG ATCCTGAACC AGTATCTGAT AATGAAGAAG ATAGTTATGA TGAGGAAATA 
CATGATCCAA GAAGTGGCCG GAGTGGTGTG GTTAACAGAA GGAGTGAGAA GATTTGGCCG 
AGGGATAGAA GTGCAAGTAG AGAGAGGAGC TTGTCCCCGC GGTCAGACAG GCGGTCAGTG 
GCTTCCAGCC AGCCCGCTAA ACCTACTAAA GTCACACTGG TGAAATCCCG GAAAAATGAA 
GAATATGGTC TTCGATTGGC AAGCCATATA TTTGTTAAGG AAATTTCACA AGATAGTTTG 
GCAGCAAGAG ATGGCAATAT TCAAGAAGGT GATGTTGTAT TGAAGATAAA TGGTACTGTG 
ACAGAAAATA TGTCATTGAC AGATGCAAAG ACATTGATAG AAAGGTCTAA AGGCAAATTA 
AAAATGGTAG TTCAAAGAGA TGAACGGGCT ACGCTATTGA ATGTCCCTGA TCTTTCTGAC 
AGCATCCACT CTGCTAATGC CTCTGAGAGA GACGACATTT CAGAAATTCA GTCACTGGCA 
TCAGATCATT CTGGTCGATC ACACGATAGG CCTCCCCGCC GCAGCCGGTC ACGATCTCCT 
GACCAGCGGT CAGAGCCTTC TGATCATTCC AGGCACTCGC CACAGCAGCC AAGCAATGGC 
AGTCTCCGGA GTAGAGATGA AGAGAGAATT TCTAAACCTG GGGCTGTCTC AACTCCTGTA 
AAGCATGCTG ATGATCACAC ACCTAAAACA GTGGAAGAAG TTACAGTTGA AAGAAATGAG 
AAACAAACAC CTTCTCTTCC AGAACCAAAG CCTGTGTATG CCCAAGTTGG GCAGCCAGAT 
GTGGATTTAC CTGTCAGTCC ATCTGATGGT GTCCTACCTA ATTCAACTCA TGAAGATGGG 
ATTCTTCGGC CCAGCATGAA ATTGGTAAAA TTCAGAAAAG GAGATAGTGT GGGTTTGCGG 
CTGGCTGGTG GAAATGATGT TGGAATATTT GTAGCTGGCG TTCTAGAAGA TAGCCCTGCA 
GCCAAGGAAG GCTTAGAGGA AGGTGATCAA ATTCTCAGGG TAAACAACGT AGATTTTACA 
AATATCATAA GAGAAGAAGC CGTCCTTTTC CTGCTTGACC TCCCTAAAGG AGAAGAAGTG 
ACCATATTGG CTCAGAAGAA GAAGGATGTT TATCGTCGCA TTGTAGAATC AGATGTAGGA 
GATTCTTTCT ATATTAGAAC CCATTTTGAA TATGAAAAGG AATCTCCCTA TGGACTTAGT 
TTTAACAAAG GAGAGGTGTT CCGTGTTGTG GATACCTTGT ACAATGGAAA ACTGGGCTCT 
TGGCTTGCTA TTCGAATTGG TAAAAATCAT AAGGAGGTAG AACGAGGCAT CATCCCTAAT 
AAGAACAGAG CTGAGCAGCT AGCCAGTGTA CAGTATACAC TTCCAAAAAC AGCAGGCGGA 
GACCGTGCTG ACTTCTGGAG ATTCAGAGGT CTTCGCAGCT CCAAGAGAAA TCTTCGAAAA 
AGCAGAGAGG ATTTGTCCGC TCAGCCTGTT CAAACAAAGT TTCCAGCTTA TGAAAGAGTG 
GTTCTTCGAG AAGCTGGATT TCTGAGGCCT GTAACCATTT TTGGACCAAT AGCTGATGTT 
GCCAGAGAAA AGCTGGCAAG AGAAGAACCA GATATTTATC AAATTGCAAA GAGTGAACCA 
CGAGACGCTG GAACTGACCA ACGTAGCTCT GGCATTATTC GCCTGCATAC AATAAAGCAA 
ATCATAGATC AAGACAAACA TGCTTTATTA GATGTAACAC CAAATGCAGT TGATCGTCTT 
AACTATGCCC AGTGGTATCC AATTGTTGTA TTTCTTAACC CTGATTCTAA GCAAGGAGTA 
AAAACAATGA GAATGAGGTT ATGTCCAGAA TCTCGGAAAA GTGCCAGGAA GTTATATGAG 
CGATCTCATA AACTTCGTAA AAATAATCAC CATCTTTTTA CAACTACAAT TAACTTAAAT 
TCAATGAATG ATGGTTGGTA TGGTGCGCTG AAAGAAGCAA TTCAACAACA GCAAAACCAG 
CTGGTATGGG TTTCCGAGGG AAAGGCGGAT GGTGCTACAA GTGATGACCT TGATTTGCAT 
GATGATCGTC TGTCCTACCT GTCAGCTCCA GGTAGTGAAT ACTCAATGTA TAGCACGGAC 
AGTAGACACA CTTCTGACTA TGAAGACACA GACACAGAAG GCGGGGCCTA CACTGATCAA 
GAACTAGATG AAACTCTTAA TGATGAGGTT GGGACTCCAC CGGAGTCTGC CATTACACGG 
TCCTCTGAGC CTGTAAGAGA GGACTCCTCT GGAATGCATC ATGAAAACCA AACATATCCT 
CCTTACTCAC CACAAGCGCA GCCACAACCA ATTCATAGAA TAGACTCCCC TGGATTTAAG 
CCAGCCTCTC AACAGAAAGC AGAAGCTTCA TCTCCAGTCC CTTACCTTTC GCCTGAAACA 
AACCCAGCAT CATCAACCTC TGCTGTTAAT CATAATGTAA ATTTAACTAA TGTCAGACTG 
GAGGAGCCCA CCCCAGCTCC TTCCACCTCT TACTCACCAC AAGCTGATTC TTTAAGAACA 
CCAAGTACTG AGGCAGCTCA CATAATGCTA AGAGATCAAG AACCATCATT GTCGTCGCAT 
GTAGATCCAA CAAAGGTGTA TAGAAAGGAT CCATATCCCG AGGAAATGAT GAGGCAGAAC 
CATGTTTTGA AACAGCCAGC CGTTAGTCAC CCAGGGCACA GGCCAGACAA AGAGCCTAAT 
CTGACCTATG AACCCCAACT CCCATACGTA GAGAAACAAG CCAGCAGAGA CCTCGAGCAG 
CCCACATACA GATACGAGTC CTCAAGCTAT ACGGACCAGT TTTCTCGAAA CTATGAACAT 
CGTCTGCGAT ACGAAGATCG CGTCCCCATG TATGAAGAAC AGTGGTCATA TTATGATGAC 
AAACAGCCCT ACCCATCTCG GCCACCTTTT GATAATCAGC ACTCTCAAGA CCTTGACTCC 
AGACAGCATC CCGAAGAGTC CTCAGAACGA GGGTACTTTC CACGTTTTGA AGAGCCAGCC 
CCTCTGTCTT ACGACAGCAG ACCACGTTAC GAACAGGCAC CTAGAGCATC CGCCCTGCGG 
CACGAAGAGC AGCCAGCTCC TGGGTATGAC ACACATGGTA GACTCAGACC GGAAGCCCAG 
CCCCACCCTT CAGCAGGGCC CAAGCCCGCA GAGTCCAAGC AGTATTTTGA GCAATATTCA 
CGCAGTTACG AGCAAGTACC ACCCCAAGGA TTTACCTCTA GAGCAGGTCA TTTTGAGCCT 
CTCCATGGTG CTGCAGCTGT CCCTCCGCTG ATACCTTCAT CTCAGCATAA GCCAGAAGCT 
CTGCCTTCAA ACACCAAACC ACTGCCTCCA CCCCCAACTC AAACCGAAGA AGAGGAAGAT 
CCAGCAATGA AGCCACAGTC TGTACTCACC AGAGTTAAGA TGTTTGAAAA CAAAAGATCT 
GCATCCTTAG AGACCAAGAA GGATGTAAAT GACACTGGCA GTTTTAAGCC TCCAGAAGTA 
GCATCTAAAC CTTCAGGTGC TCCCATCATT GGTCCCAAAC CCACTTCTCA GAATCAGTTC 
AGTGAACATG ACAAAACTCT GTACAGGATC CCAGAACCTC AAAAACCTCA ACTGAAGCCA 
CCTGAAGATA TCGTTCGGTC CAATCATTAT GACCCTGAAG AAGATGAAGA ATATTATCGA 
AAACAGCTGT CATACTTTGA CCGAAGAAGT TTTGAGAATA AGCCTCCTGC ACACATTGCC 
GCCAGCCATC TCTCCGAGCC TGCAAAGCCA GCTCATTCTC AGAATCAATC AAATTTTTCT 
AGTTATTCTT CAAAGGGAAA GCCTCCTGAA GCTGATGGTG TGGATAGATC ATTTGGCGAG 
AAACGCTATG AACCCATCCA GGCCACTCCC CCTCCTCCTC CATTGCCCTC GCAGTATGCC 
CAGCCATCTC AGCCTGTCAC CAGCGCGTCT CTCCACATAC ATTCTAAGGG AGCACATGGT 
GAAGGTAATT CAGTGTCATT GGATTTTCAG AATTCCTTAG TGTCCAAACC AGACCCACCT 
CCATCTCAGA ATAAGCCAGC AACTTTCAGA CCACCAAACC GAGAAGATAC TGCTCAGCCA 
GCTTTCTATC CCCAGAAAAG TTTTCCAGAT AAAGCCCCAG TTAATGGAAC TGAACAGACT 
CAGAAAACAG TCACTCCAGC ATACAATCGA TTCACACCAA AACCATATAC AAGTTCTGCC 
CGACCATTTG AACGCAAGTT TGAAAGTCCT AAATTCAATC ACAATCTTCT GCCAAGTGAA 
ACTGCACATA AACCTGAATT GTCTTCAAAA ACTCCCACTT CTCCAAAAAC TCTTGTGAAA 
TCGCACAGTT TGGCACAGCC TCCTGAGTTT GACAGTGGAG TTGAAACTTT CTCTATCCAT 
GCAGAGAAGC CTAAATATCA AATAAATAAT ATCAGCACAG TGCCTAAAGC TATTCCTGTG 
AGTCCTTCAG CTGTGGAAGA GGATGAAGAT GAAGATGGTC ATACTGTGGT GGCCACAGCC 
CGAGGCATAT TTAACAGCAA TGGGGGCGTG CTGAGTTCCA TAGAAACTGG CGTTAGTATA 
ATTATCCCTC AAGGAGCCAT TCCCGAAGGA GTTGAGCAGG AAATCTATTT CAAGGTCTGC 
CGGGACAACA GCATCCTTCC ACCTTTAGAT AAAGAGAAAG GTGAAACACT GCTGAGTCCT 
TTGGTGATGT GTGGTCCCCA TGGCCTCAAG TTCCTGAAGC CTGTGGAGCT GCGCTTACCA 
CACTGTGCGT CCATGACTCC TGACGGTTGG TCTTTTGCTC TAAAATCATC CGACTCCTCG 
TCGGGTGATC CTAAAACCTG GCAAAACAAG TGTCTTCCCG GAGATCCAAA TTATCTCGTT 
GGAGCAAACT GTGTTTCTGT CCTTATTGAC CACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_009428688.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009426963.2 Pan troglodytes tight junction protein 1 (TJP1), transcript variant X6, mRNA.

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CloneID	OPc69379
Clone ID Related Accession (Same CDS sequence)	XM_009428688.2
Accession Version	XM_009428688.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5307bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	tight junction protein ZO-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006482.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009428688.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGGT TTGGAATTGC AATATCTGGT 
GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG 
AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA 
GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT 
CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA 
AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT 
GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG 
CCCGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT 
CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT 
GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT 
GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA 
GAGCCTTCTG ATCATTCCAG GCACTCGCCA CAGCAGCCAA GCAATGGCAG TCTCCGGAGT 
AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT 
GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT 
TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AGCCAGATGT GGATTTACCT 
GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC 
AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA 
AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA 
GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT 
CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC 
TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT 
TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA 
GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA 
GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG 
TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA 
ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATATGAGCG ATCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT 
TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA 
CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA 
CAGAAAGCAG AAGCTTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA 
TCAACCTCTG CTGTTAATCA TAATGTAAAT TTAACTAATG TCAGACTGGA GGAGCCCACC 
CCAGCTCCTT CCACCTCTTA CTCACCACAA GCTGATTCTT TAAGAACACC AAGTACTGAG 
GCAGCTCACA TAATGCTAAG AGATCAAGAA CCATCATTGT CGTCGCATGT AGATCCAACA 
AAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA 
CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA 
CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA 
TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC 
GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC 
GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC 
GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG 
CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA 
GCAGGGCCCA AGCCCGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG 
CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT 
GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC 
ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG 
CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG 
ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT 
TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAGTTCAG TGAACATGAC 
AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATC 
GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA 
TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC 
TCCGAGCCTG CAAAGCCAGC TCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA 
AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA 
CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG 
CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA 
GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT 
AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGCCAGC TTTCTATCCC 
CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC 
ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA 
CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA 
CCTGAATTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG 
GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT 
AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT 
GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT 
AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGCG TTAGTATAAT TATCCCTCAA 
GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC 
ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT 
GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGCGTCC 
ATGACTCCTG ACGGTTGGTC TTTTGCTCTA AAATCATCCG ACTCCTCGTC GGGTGATCCT 
AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT 
GTTTCTGTCC TTATTGACCA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009426963.2
CDS	470..5776
Translation	MSARAAAAKSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQKAEASSPVPYLSPETNPASSTSAVNHNVNLTNVRLEEPTPAPSTSYSPQADSLRTPSTEAAHIMLRDQEPSLSSHVDPTKVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQPAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPELSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_009428688.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes tight junction protein 1 (TJP1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009428688.2

ORF Insert Sequence:

ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGGT TTGGAATTGC AATATCTGGT 
GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG 
AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA 
GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT 
CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA 
AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT 
GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG 
CCCGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT 
CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT 
GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT 
GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA 
GAGCCTTCTG ATCATTCCAG GCACTCGCCA CAGCAGCCAA GCAATGGCAG TCTCCGGAGT 
AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT 
GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT 
TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AGCCAGATGT GGATTTACCT 
GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC 
AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA 
AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA 
GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT 
CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC 
TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT 
TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA 
GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA 
GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG 
TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA 
ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATATGAGCG ATCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT 
TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA 
CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA 
CAGAAAGCAG AAGCTTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA 
TCAACCTCTG CTGTTAATCA TAATGTAAAT TTAACTAATG TCAGACTGGA GGAGCCCACC 
CCAGCTCCTT CCACCTCTTA CTCACCACAA GCTGATTCTT TAAGAACACC AAGTACTGAG 
GCAGCTCACA TAATGCTAAG AGATCAAGAA CCATCATTGT CGTCGCATGT AGATCCAACA 
AAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA 
CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA 
CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA 
TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC 
GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC 
GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC 
GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG 
CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA 
GCAGGGCCCA AGCCCGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG 
CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT 
GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC 
ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG 
CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG 
ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT 
TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAGTTCAG TGAACATGAC 
AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATC 
GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA 
TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC 
TCCGAGCCTG CAAAGCCAGC TCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA 
AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA 
CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG 
CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA 
GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT 
AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGCCAGC TTTCTATCCC 
CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC 
ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA 
CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA 
CCTGAATTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG 
GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT 
AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT 
GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT 
AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGCG TTAGTATAAT TATCCCTCAA 
GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC 
ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT 
GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGCGTCC 
ATGACTCCTG ACGGTTGGTC TTTTGCTCTA AAATCATCCG ACTCCTCGTC GGGTGATCCT 
AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT 
GTTTCTGTCC TTATTGACCA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_016927839.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016783328.1 Pan troglodytes tight junction protein 1 (TJP1), transcript variant X7, mRNA.

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CloneID	OPc69380
Clone ID Related Accession (Same CDS sequence)	XM_016927839.1
Accession Version	XM_016927839.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5301bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	tight junction protein ZO-1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006482.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTATT ACTTATTTAA GAGCACAGCA ATGGAGGAAA CAGCTATATG GGAACAACAT 
ACAGTGACGC TTCACAGGGC TCCTGGATTT GGGTTTGGAA TTGCAATATC TGGTGGACGA 
GATAATCCTC ATTTTCAGAG TGGGGAAACG TCAATAGTGA TTTCAGATGT GCTGAAAGGA 
GGACCAGCTG AAGGACAGCT ACAGGAAAAT GACCGAGTTG CAATGGTTAA CGGAGTTTCA 
ATGGATAATG TTGAACATGC TTTTGCTGTT CAGCAACTAA GGAAAAGTGG GAAAAATGCA 
AAAATTACAA TTAGAAGGAA GAAGAAAGTT CAAATACCAG TAAGTCGTCC TGATCCTGAA 
CCAGTATCTG ATAATGAAGA AGATAGTTAT GATGAGGAAA TACATGATCC AAGAAGTGGC 
CGGAGTGGTG TGGTTAACAG AAGGAGTGAG AAGATTTGGC CGAGGGATAG AAGTGCAAGT 
AGAGAGAGGA GCTTGTCCCC GCGGTCAGAC AGGCGGTCAG TGGCTTCCAG CCAGCCCGCT 
AAACCTACTA AAGTCACACT GGTGAAATCC CGGAAAAATG AAGAATATGG TCTTCGATTG 
GCAAGCCATA TATTTGTTAA GGAAATTTCA CAAGATAGTT TGGCAGCAAG AGATGGCAAT 
ATTCAAGAAG GTGATGTTGT ATTGAAGATA AATGGTACTG TGACAGAAAA TATGTCATTG 
ACAGATGCAA AGACATTGAT AGAAAGGTCT AAAGGCAAAT TAAAAATGGT AGTTCAAAGA 
GATGAACGGG CTACGCTATT GAATGTCCCT GATCTTTCTG ACAGCATCCA CTCTGCTAAT 
GCCTCTGAGA GAGACGACAT TTCAGAAATT CAGTCACTGG CATCAGATCA TTCTGGTCGA 
TCACACGATA GGCCTCCCCG CCGCAGCCGG TCACGATCTC CTGACCAGCG GTCAGAGCCT 
TCTGATCATT CCAGGCACTC GCCACAGCAG CCAAGCAATG GCAGTCTCCG GAGTAGAGAT 
GAAGAGAGAA TTTCTAAACC TGGGGCTGTC TCAACTCCTG TAAAGCATGC TGATGATCAC 
ACACCTAAAA CAGTGGAAGA AGTTACAGTT GAAAGAAATG AGAAACAAAC ACCTTCTCTT 
CCAGAACCAA AGCCTGTGTA TGCCCAAGTT GGGCAGCCAG ATGTGGATTT ACCTGTCAGT 
CCATCTGATG GTGTCCTACC TAATTCAACT CATGAAGATG GGATTCTTCG GCCCAGCATG 
AAATTGGTAA AATTCAGAAA AGGAGATAGT GTGGGTTTGC GGCTGGCTGG TGGAAATGAT 
GTTGGAATAT TTGTAGCTGG CGTTCTAGAA GATAGCCCTG CAGCCAAGGA AGGCTTAGAG 
GAAGGTGATC AAATTCTCAG GGTAAACAAC GTAGATTTTA CAAATATCAT AAGAGAAGAA 
GCCGTCCTTT TCCTGCTTGA CCTCCCTAAA GGAGAAGAAG TGACCATATT GGCTCAGAAG 
AAGAAGGATG TTTATCGTCG CATTGTAGAA TCAGATGTAG GAGATTCTTT CTATATTAGA 
ACCCATTTTG AATATGAAAA GGAATCTCCC TATGGACTTA GTTTTAACAA AGGAGAGGTG 
TTCCGTGTTG TGGATACCTT GTACAATGGA AAACTGGGCT CTTGGCTTGC TATTCGAATT 
GGTAAAAATC ATAAGGAGGT AGAACGAGGC ATCATCCCTA ATAAGAACAG AGCTGAGCAG 
CTAGCCAGTG TACAGTATAC ACTTCCAAAA ACAGCAGGCG GAGACCGTGC TGACTTCTGG 
AGATTCAGAG GTCTTCGCAG CTCCAAGAGA AATCTTCGAA AAAGCAGAGA GGATTTGTCC 
GCTCAGCCTG TTCAAACAAA GTTTCCAGCT TATGAAAGAG TGGTTCTTCG AGAAGCTGGA 
TTTCTGAGGC CTGTAACCAT TTTTGGACCA ATAGCTGATG TTGCCAGAGA AAAGCTGGCA 
AGAGAAGAAC CAGATATTTA TCAAATTGCA AAGAGTGAAC CACGAGACGC TGGAACTGAC 
CAACGTAGCT CTGGCATTAT TCGCCTGCAT ACAATAAAGC AAATCATAGA TCAAGACAAA 
CATGCTTTAT TAGATGTAAC ACCAAATGCA GTTGATCGTC TTAACTATGC CCAGTGGTAT 
CCAATTGTTG TATTTCTTAA CCCTGATTCT AAGCAAGGAG TAAAAACAAT GAGAATGAGG 
TTATGTCCAG AATCTCGGAA AAGTGCCAGG AAGTTATATG AGCGATCTCA TAAACTTCGT 
AAAAATAATC ACCATCTTTT TACAACTACA ATTAACTTAA ATTCAATGAA TGATGGTTGG 
TATGGTGCGC TGAAAGAAGC AATTCAACAA CAGCAAAACC AGCTGGTATG GGTTTCCGAG 
GGAAAGGCGG ATGGTGCTAC AAGTGATGAC CTTGATTTGC ATGATGATCG TCTGTCCTAC 
CTGTCAGCTC CAGGTAGTGA ATACTCAATG TATAGCACGG ACAGTAGACA CACTTCTGAC 
TATGAAGACA CAGACACAGA AGGCGGGGCC TACACTGATC AAGAACTAGA TGAAACTCTT 
AATGATGAGG TTGGGACTCC ACCGGAGTCT GCCATTACAC GGTCCTCTGA GCCTGTAAGA 
GAGGACTCCT CTGGAATGCA TCATGAAAAC CAAACATATC CTCCTTACTC ACCACAAGCG 
CAGCCACAAC CAATTCATAG AATAGACTCC CCTGGATTTA AGCCAGCCTC TCAACAGAAA 
GCAGAAGCTT CATCTCCAGT CCCTTACCTT TCGCCTGAAA CAAACCCAGC ATCATCAACC 
TCTGCTGTTA ATCATAATGT AAATTTAACT AATGTCAGAC TGGAGGAGCC CACCCCAGCT 
CCTTCCACCT CTTACTCACC ACAAGCTGAT TCTTTAAGAA CACCAAGTAC TGAGGCAGCT 
CACATAATGC TAAGAGATCA AGAACCATCA TTGTCGTCGC ATGTAGATCC AACAAAGGTG 
TATAGAAAGG ATCCATATCC CGAGGAAATG ATGAGGCAGA ACCATGTTTT GAAACAGCCA 
GCCGTTAGTC ACCCAGGGCA CAGGCCAGAC AAAGAGCCTA ATCTGACCTA TGAACCCCAA 
CTCCCATACG TAGAGAAACA AGCCAGCAGA GACCTCGAGC AGCCCACATA CAGATACGAG 
TCCTCAAGCT ATACGGACCA GTTTTCTCGA AACTATGAAC ATCGTCTGCG ATACGAAGAT 
CGCGTCCCCA TGTATGAAGA ACAGTGGTCA TATTATGATG ACAAACAGCC CTACCCATCT 
CGGCCACCTT TTGATAATCA GCACTCTCAA GACCTTGACT CCAGACAGCA TCCCGAAGAG 
TCCTCAGAAC GAGGGTACTT TCCACGTTTT GAAGAGCCAG CCCCTCTGTC TTACGACAGC 
AGACCACGTT ACGAACAGGC ACCTAGAGCA TCCGCCCTGC GGCACGAAGA GCAGCCAGCT 
CCTGGGTATG ACACACATGG TAGACTCAGA CCGGAAGCCC AGCCCCACCC TTCAGCAGGG 
CCCAAGCCCG CAGAGTCCAA GCAGTATTTT GAGCAATATT CACGCAGTTA CGAGCAAGTA 
CCACCCCAAG GATTTACCTC TAGAGCAGGT CATTTTGAGC CTCTCCATGG TGCTGCAGCT 
GTCCCTCCGC TGATACCTTC ATCTCAGCAT AAGCCAGAAG CTCTGCCTTC AAACACCAAA 
CCACTGCCTC CACCCCCAAC TCAAACCGAA GAAGAGGAAG ATCCAGCAAT GAAGCCACAG 
TCTGTACTCA CCAGAGTTAA GATGTTTGAA AACAAAAGAT CTGCATCCTT AGAGACCAAG 
AAGGATGTAA ATGACACTGG CAGTTTTAAG CCTCCAGAAG TAGCATCTAA ACCTTCAGGT 
GCTCCCATCA TTGGTCCCAA ACCCACTTCT CAGAATCAGT TCAGTGAACA TGACAAAACT 
CTGTACAGGA TCCCAGAACC TCAAAAACCT CAACTGAAGC CACCTGAAGA TATCGTTCGG 
TCCAATCATT ATGACCCTGA AGAAGATGAA GAATATTATC GAAAACAGCT GTCATACTTT 
GACCGAAGAA GTTTTGAGAA TAAGCCTCCT GCACACATTG CCGCCAGCCA TCTCTCCGAG 
CCTGCAAAGC CAGCTCATTC TCAGAATCAA TCAAATTTTT CTAGTTATTC TTCAAAGGGA 
AAGCCTCCTG AAGCTGATGG TGTGGATAGA TCATTTGGCG AGAAACGCTA TGAACCCATC 
CAGGCCACTC CCCCTCCTCC TCCATTGCCC TCGCAGTATG CCCAGCCATC TCAGCCTGTC 
ACCAGCGCGT CTCTCCACAT ACATTCTAAG GGAGCACATG GTGAAGGTAA TTCAGTGTCA 
TTGGATTTTC AGAATTCCTT AGTGTCCAAA CCAGACCCAC CTCCATCTCA GAATAAGCCA 
GCAACTTTCA GACCACCAAA CCGAGAAGAT ACTGCTCAGC CAGCTTTCTA TCCCCAGAAA 
AGTTTTCCAG ATAAAGCCCC AGTTAATGGA ACTGAACAGA CTCAGAAAAC AGTCACTCCA 
GCATACAATC GATTCACACC AAAACCATAT ACAAGTTCTG CCCGACCATT TGAACGCAAG 
TTTGAAAGTC CTAAATTCAA TCACAATCTT CTGCCAAGTG AAACTGCACA TAAACCTGAA 
TTGTCTTCAA AAACTCCCAC TTCTCCAAAA ACTCTTGTGA AATCGCACAG TTTGGCACAG 
CCTCCTGAGT TTGACAGTGG AGTTGAAACT TTCTCTATCC ATGCAGAGAA GCCTAAATAT 
CAAATAAATA ATATCAGCAC AGTGCCTAAA GCTATTCCTG TGAGTCCTTC AGCTGTGGAA 
GAGGATGAAG ATGAAGATGG TCATACTGTG GTGGCCACAG CCCGAGGCAT ATTTAACAGC 
AATGGGGGCG TGCTGAGTTC CATAGAAACT GGCGTTAGTA TAATTATCCC TCAAGGAGCC 
ATTCCCGAAG GAGTTGAGCA GGAAATCTAT TTCAAGGTCT GCCGGGACAA CAGCATCCTT 
CCACCTTTAG ATAAAGAGAA AGGTGAAACA CTGCTGAGTC CTTTGGTGAT GTGTGGTCCC 
CATGGCCTCA AGTTCCTGAA GCCTGTGGAG CTGCGCTTAC CACACTGTGC GTCCATGACT 
CCTGACGGTT GGTCTTTTGC TCTAAAATCA TCCGACTCCT CGTCGGGTGA TCCTAAAACC 
TGGCAAAACA AGTGTCTTCC CGGAGATCCA AATTATCTCG TTGGAGCAAA CTGTGTTTCT 
GTCCTTATTG ACCACTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016783328.1
CDS	16..5316
Translation	MLYYLFKSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQKAEASSPVPYLSPETNPASSTSAVNHNVNLTNVRLEEPTPAPSTSYSPQADSLRTPSTEAAHIMLRDQEPSLSSHVDPTKVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQPAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPELSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_016927839.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes tight junction protein 1 (TJP1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016927839.1

ORF Insert Sequence:

ATGCTGTATT ACTTATTTAA GAGCACAGCA ATGGAGGAAA CAGCTATATG GGAACAACAT 
ACAGTGACGC TTCACAGGGC TCCTGGATTT GGGTTTGGAA TTGCAATATC TGGTGGACGA 
GATAATCCTC ATTTTCAGAG TGGGGAAACG TCAATAGTGA TTTCAGATGT GCTGAAAGGA 
GGACCAGCTG AAGGACAGCT ACAGGAAAAT GACCGAGTTG CAATGGTTAA CGGAGTTTCA 
ATGGATAATG TTGAACATGC TTTTGCTGTT CAGCAACTAA GGAAAAGTGG GAAAAATGCA 
AAAATTACAA TTAGAAGGAA GAAGAAAGTT CAAATACCAG TAAGTCGTCC TGATCCTGAA 
CCAGTATCTG ATAATGAAGA AGATAGTTAT GATGAGGAAA TACATGATCC AAGAAGTGGC 
CGGAGTGGTG TGGTTAACAG AAGGAGTGAG AAGATTTGGC CGAGGGATAG AAGTGCAAGT 
AGAGAGAGGA GCTTGTCCCC GCGGTCAGAC AGGCGGTCAG TGGCTTCCAG CCAGCCCGCT 
AAACCTACTA AAGTCACACT GGTGAAATCC CGGAAAAATG AAGAATATGG TCTTCGATTG 
GCAAGCCATA TATTTGTTAA GGAAATTTCA CAAGATAGTT TGGCAGCAAG AGATGGCAAT 
ATTCAAGAAG GTGATGTTGT ATTGAAGATA AATGGTACTG TGACAGAAAA TATGTCATTG 
ACAGATGCAA AGACATTGAT AGAAAGGTCT AAAGGCAAAT TAAAAATGGT AGTTCAAAGA 
GATGAACGGG CTACGCTATT GAATGTCCCT GATCTTTCTG ACAGCATCCA CTCTGCTAAT 
GCCTCTGAGA GAGACGACAT TTCAGAAATT CAGTCACTGG CATCAGATCA TTCTGGTCGA 
TCACACGATA GGCCTCCCCG CCGCAGCCGG TCACGATCTC CTGACCAGCG GTCAGAGCCT 
TCTGATCATT CCAGGCACTC GCCACAGCAG CCAAGCAATG GCAGTCTCCG GAGTAGAGAT 
GAAGAGAGAA TTTCTAAACC TGGGGCTGTC TCAACTCCTG TAAAGCATGC TGATGATCAC 
ACACCTAAAA CAGTGGAAGA AGTTACAGTT GAAAGAAATG AGAAACAAAC ACCTTCTCTT 
CCAGAACCAA AGCCTGTGTA TGCCCAAGTT GGGCAGCCAG ATGTGGATTT ACCTGTCAGT 
CCATCTGATG GTGTCCTACC TAATTCAACT CATGAAGATG GGATTCTTCG GCCCAGCATG 
AAATTGGTAA AATTCAGAAA AGGAGATAGT GTGGGTTTGC GGCTGGCTGG TGGAAATGAT 
GTTGGAATAT TTGTAGCTGG CGTTCTAGAA GATAGCCCTG CAGCCAAGGA AGGCTTAGAG 
GAAGGTGATC AAATTCTCAG GGTAAACAAC GTAGATTTTA CAAATATCAT AAGAGAAGAA 
GCCGTCCTTT TCCTGCTTGA CCTCCCTAAA GGAGAAGAAG TGACCATATT GGCTCAGAAG 
AAGAAGGATG TTTATCGTCG CATTGTAGAA TCAGATGTAG GAGATTCTTT CTATATTAGA 
ACCCATTTTG AATATGAAAA GGAATCTCCC TATGGACTTA GTTTTAACAA AGGAGAGGTG 
TTCCGTGTTG TGGATACCTT GTACAATGGA AAACTGGGCT CTTGGCTTGC TATTCGAATT 
GGTAAAAATC ATAAGGAGGT AGAACGAGGC ATCATCCCTA ATAAGAACAG AGCTGAGCAG 
CTAGCCAGTG TACAGTATAC ACTTCCAAAA ACAGCAGGCG GAGACCGTGC TGACTTCTGG 
AGATTCAGAG GTCTTCGCAG CTCCAAGAGA AATCTTCGAA AAAGCAGAGA GGATTTGTCC 
GCTCAGCCTG TTCAAACAAA GTTTCCAGCT TATGAAAGAG TGGTTCTTCG AGAAGCTGGA 
TTTCTGAGGC CTGTAACCAT TTTTGGACCA ATAGCTGATG TTGCCAGAGA AAAGCTGGCA 
AGAGAAGAAC CAGATATTTA TCAAATTGCA AAGAGTGAAC CACGAGACGC TGGAACTGAC 
CAACGTAGCT CTGGCATTAT TCGCCTGCAT ACAATAAAGC AAATCATAGA TCAAGACAAA 
CATGCTTTAT TAGATGTAAC ACCAAATGCA GTTGATCGTC TTAACTATGC CCAGTGGTAT 
CCAATTGTTG TATTTCTTAA CCCTGATTCT AAGCAAGGAG TAAAAACAAT GAGAATGAGG 
TTATGTCCAG AATCTCGGAA AAGTGCCAGG AAGTTATATG AGCGATCTCA TAAACTTCGT 
AAAAATAATC ACCATCTTTT TACAACTACA ATTAACTTAA ATTCAATGAA TGATGGTTGG 
TATGGTGCGC TGAAAGAAGC AATTCAACAA CAGCAAAACC AGCTGGTATG GGTTTCCGAG 
GGAAAGGCGG ATGGTGCTAC AAGTGATGAC CTTGATTTGC ATGATGATCG TCTGTCCTAC 
CTGTCAGCTC CAGGTAGTGA ATACTCAATG TATAGCACGG ACAGTAGACA CACTTCTGAC 
TATGAAGACA CAGACACAGA AGGCGGGGCC TACACTGATC AAGAACTAGA TGAAACTCTT 
AATGATGAGG TTGGGACTCC ACCGGAGTCT GCCATTACAC GGTCCTCTGA GCCTGTAAGA 
GAGGACTCCT CTGGAATGCA TCATGAAAAC CAAACATATC CTCCTTACTC ACCACAAGCG 
CAGCCACAAC CAATTCATAG AATAGACTCC CCTGGATTTA AGCCAGCCTC TCAACAGAAA 
GCAGAAGCTT CATCTCCAGT CCCTTACCTT TCGCCTGAAA CAAACCCAGC ATCATCAACC 
TCTGCTGTTA ATCATAATGT AAATTTAACT AATGTCAGAC TGGAGGAGCC CACCCCAGCT 
CCTTCCACCT CTTACTCACC ACAAGCTGAT TCTTTAAGAA CACCAAGTAC TGAGGCAGCT 
CACATAATGC TAAGAGATCA AGAACCATCA TTGTCGTCGC ATGTAGATCC AACAAAGGTG 
TATAGAAAGG ATCCATATCC CGAGGAAATG ATGAGGCAGA ACCATGTTTT GAAACAGCCA 
GCCGTTAGTC ACCCAGGGCA CAGGCCAGAC AAAGAGCCTA ATCTGACCTA TGAACCCCAA 
CTCCCATACG TAGAGAAACA AGCCAGCAGA GACCTCGAGC AGCCCACATA CAGATACGAG 
TCCTCAAGCT ATACGGACCA GTTTTCTCGA AACTATGAAC ATCGTCTGCG ATACGAAGAT 
CGCGTCCCCA TGTATGAAGA ACAGTGGTCA TATTATGATG ACAAACAGCC CTACCCATCT 
CGGCCACCTT TTGATAATCA GCACTCTCAA GACCTTGACT CCAGACAGCA TCCCGAAGAG 
TCCTCAGAAC GAGGGTACTT TCCACGTTTT GAAGAGCCAG CCCCTCTGTC TTACGACAGC 
AGACCACGTT ACGAACAGGC ACCTAGAGCA TCCGCCCTGC GGCACGAAGA GCAGCCAGCT 
CCTGGGTATG ACACACATGG TAGACTCAGA CCGGAAGCCC AGCCCCACCC TTCAGCAGGG 
CCCAAGCCCG CAGAGTCCAA GCAGTATTTT GAGCAATATT CACGCAGTTA CGAGCAAGTA 
CCACCCCAAG GATTTACCTC TAGAGCAGGT CATTTTGAGC CTCTCCATGG TGCTGCAGCT 
GTCCCTCCGC TGATACCTTC ATCTCAGCAT AAGCCAGAAG CTCTGCCTTC AAACACCAAA 
CCACTGCCTC CACCCCCAAC TCAAACCGAA GAAGAGGAAG ATCCAGCAAT GAAGCCACAG 
TCTGTACTCA CCAGAGTTAA GATGTTTGAA AACAAAAGAT CTGCATCCTT AGAGACCAAG 
AAGGATGTAA ATGACACTGG CAGTTTTAAG CCTCCAGAAG TAGCATCTAA ACCTTCAGGT 
GCTCCCATCA TTGGTCCCAA ACCCACTTCT CAGAATCAGT TCAGTGAACA TGACAAAACT 
CTGTACAGGA TCCCAGAACC TCAAAAACCT CAACTGAAGC CACCTGAAGA TATCGTTCGG 
TCCAATCATT ATGACCCTGA AGAAGATGAA GAATATTATC GAAAACAGCT GTCATACTTT 
GACCGAAGAA GTTTTGAGAA TAAGCCTCCT GCACACATTG CCGCCAGCCA TCTCTCCGAG 
CCTGCAAAGC CAGCTCATTC TCAGAATCAA TCAAATTTTT CTAGTTATTC TTCAAAGGGA 
AAGCCTCCTG AAGCTGATGG TGTGGATAGA TCATTTGGCG AGAAACGCTA TGAACCCATC 
CAGGCCACTC CCCCTCCTCC TCCATTGCCC TCGCAGTATG CCCAGCCATC TCAGCCTGTC 
ACCAGCGCGT CTCTCCACAT ACATTCTAAG GGAGCACATG GTGAAGGTAA TTCAGTGTCA 
TTGGATTTTC AGAATTCCTT AGTGTCCAAA CCAGACCCAC CTCCATCTCA GAATAAGCCA 
GCAACTTTCA GACCACCAAA CCGAGAAGAT ACTGCTCAGC CAGCTTTCTA TCCCCAGAAA 
AGTTTTCCAG ATAAAGCCCC AGTTAATGGA ACTGAACAGA CTCAGAAAAC AGTCACTCCA 
GCATACAATC GATTCACACC AAAACCATAT ACAAGTTCTG CCCGACCATT TGAACGCAAG 
TTTGAAAGTC CTAAATTCAA TCACAATCTT CTGCCAAGTG AAACTGCACA TAAACCTGAA 
TTGTCTTCAA AAACTCCCAC TTCTCCAAAA ACTCTTGTGA AATCGCACAG TTTGGCACAG 
CCTCCTGAGT TTGACAGTGG AGTTGAAACT TTCTCTATCC ATGCAGAGAA GCCTAAATAT 
CAAATAAATA ATATCAGCAC AGTGCCTAAA GCTATTCCTG TGAGTCCTTC AGCTGTGGAA 
GAGGATGAAG ATGAAGATGG TCATACTGTG GTGGCCACAG CCCGAGGCAT ATTTAACAGC 
AATGGGGGCG TGCTGAGTTC CATAGAAACT GGCGTTAGTA TAATTATCCC TCAAGGAGCC 
ATTCCCGAAG GAGTTGAGCA GGAAATCTAT TTCAAGGTCT GCCGGGACAA CAGCATCCTT 
CCACCTTTAG ATAAAGAGAA AGGTGAAACA CTGCTGAGTC CTTTGGTGAT GTGTGGTCCC 
CATGGCCTCA AGTTCCTGAA GCCTGTGGAG CTGCGCTTAC CACACTGTGC GTCCATGACT 
CCTGACGGTT GGTCTTTTGC TCTAAAATCA TCCGACTCCT CGTCGGGTGA TCCTAAAACC 
TGGCAAAACA AGTGTCTTCC CGGAGATCCA AATTATCTCG TTGGAGCAAA CTGTGTTTCT 
GTCCTTATTG ACCACTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_016927840.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016783329.1 Pan troglodytes tight junction protein 1 (TJP1), transcript variant X8, mRNA.

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CloneID	OPc69381
Clone ID Related Accession (Same CDS sequence)	XM_016927840.1
Accession Version	XM_016927840.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5298bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	tight junction protein ZO-1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006482.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTGCC ACAGTAAGAG CACAGCAATG GAGGAAACAG CTATATGGGA ACAACATACA 
GTGACGCTTC ACAGGGCTCC TGGATTTGGG TTTGGAATTG CAATATCTGG TGGACGAGAT 
AATCCTCATT TTCAGAGTGG GGAAACGTCA ATAGTGATTT CAGATGTGCT GAAAGGAGGA 
CCAGCTGAAG GACAGCTACA GGAAAATGAC CGAGTTGCAA TGGTTAACGG AGTTTCAATG 
GATAATGTTG AACATGCTTT TGCTGTTCAG CAACTAAGGA AAAGTGGGAA AAATGCAAAA 
ATTACAATTA GAAGGAAGAA GAAAGTTCAA ATACCAGTAA GTCGTCCTGA TCCTGAACCA 
GTATCTGATA ATGAAGAAGA TAGTTATGAT GAGGAAATAC ATGATCCAAG AAGTGGCCGG 
AGTGGTGTGG TTAACAGAAG GAGTGAGAAG ATTTGGCCGA GGGATAGAAG TGCAAGTAGA 
GAGAGGAGCT TGTCCCCGCG GTCAGACAGG CGGTCAGTGG CTTCCAGCCA GCCCGCTAAA 
CCTACTAAAG TCACACTGGT GAAATCCCGG AAAAATGAAG AATATGGTCT TCGATTGGCA 
AGCCATATAT TTGTTAAGGA AATTTCACAA GATAGTTTGG CAGCAAGAGA TGGCAATATT 
CAAGAAGGTG ATGTTGTATT GAAGATAAAT GGTACTGTGA CAGAAAATAT GTCATTGACA 
GATGCAAAGA CATTGATAGA AAGGTCTAAA GGCAAATTAA AAATGGTAGT TCAAAGAGAT 
GAACGGGCTA CGCTATTGAA TGTCCCTGAT CTTTCTGACA GCATCCACTC TGCTAATGCC 
TCTGAGAGAG ACGACATTTC AGAAATTCAG TCACTGGCAT CAGATCATTC TGGTCGATCA 
CACGATAGGC CTCCCCGCCG CAGCCGGTCA CGATCTCCTG ACCAGCGGTC AGAGCCTTCT 
GATCATTCCA GGCACTCGCC ACAGCAGCCA AGCAATGGCA GTCTCCGGAG TAGAGATGAA 
GAGAGAATTT CTAAACCTGG GGCTGTCTCA ACTCCTGTAA AGCATGCTGA TGATCACACA 
CCTAAAACAG TGGAAGAAGT TACAGTTGAA AGAAATGAGA AACAAACACC TTCTCTTCCA 
GAACCAAAGC CTGTGTATGC CCAAGTTGGG CAGCCAGATG TGGATTTACC TGTCAGTCCA 
TCTGATGGTG TCCTACCTAA TTCAACTCAT GAAGATGGGA TTCTTCGGCC CAGCATGAAA 
TTGGTAAAAT TCAGAAAAGG AGATAGTGTG GGTTTGCGGC TGGCTGGTGG AAATGATGTT 
GGAATATTTG TAGCTGGCGT TCTAGAAGAT AGCCCTGCAG CCAAGGAAGG CTTAGAGGAA 
GGTGATCAAA TTCTCAGGGT AAACAACGTA GATTTTACAA ATATCATAAG AGAAGAAGCC 
GTCCTTTTCC TGCTTGACCT CCCTAAAGGA GAAGAAGTGA CCATATTGGC TCAGAAGAAG 
AAGGATGTTT ATCGTCGCAT TGTAGAATCA GATGTAGGAG ATTCTTTCTA TATTAGAACC 
CATTTTGAAT ATGAAAAGGA ATCTCCCTAT GGACTTAGTT TTAACAAAGG AGAGGTGTTC 
CGTGTTGTGG ATACCTTGTA CAATGGAAAA CTGGGCTCTT GGCTTGCTAT TCGAATTGGT 
AAAAATCATA AGGAGGTAGA ACGAGGCATC ATCCCTAATA AGAACAGAGC TGAGCAGCTA 
GCCAGTGTAC AGTATACACT TCCAAAAACA GCAGGCGGAG ACCGTGCTGA CTTCTGGAGA 
TTCAGAGGTC TTCGCAGCTC CAAGAGAAAT CTTCGAAAAA GCAGAGAGGA TTTGTCCGCT 
CAGCCTGTTC AAACAAAGTT TCCAGCTTAT GAAAGAGTGG TTCTTCGAGA AGCTGGATTT 
CTGAGGCCTG TAACCATTTT TGGACCAATA GCTGATGTTG CCAGAGAAAA GCTGGCAAGA 
GAAGAACCAG ATATTTATCA AATTGCAAAG AGTGAACCAC GAGACGCTGG AACTGACCAA 
CGTAGCTCTG GCATTATTCG CCTGCATACA ATAAAGCAAA TCATAGATCA AGACAAACAT 
GCTTTATTAG ATGTAACACC AAATGCAGTT GATCGTCTTA ACTATGCCCA GTGGTATCCA 
ATTGTTGTAT TTCTTAACCC TGATTCTAAG CAAGGAGTAA AAACAATGAG AATGAGGTTA 
TGTCCAGAAT CTCGGAAAAG TGCCAGGAAG TTATATGAGC GATCTCATAA ACTTCGTAAA 
AATAATCACC ATCTTTTTAC AACTACAATT AACTTAAATT CAATGAATGA TGGTTGGTAT 
GGTGCGCTGA AAGAAGCAAT TCAACAACAG CAAAACCAGC TGGTATGGGT TTCCGAGGGA 
AAGGCGGATG GTGCTACAAG TGATGACCTT GATTTGCATG ATGATCGTCT GTCCTACCTG 
TCAGCTCCAG GTAGTGAATA CTCAATGTAT AGCACGGACA GTAGACACAC TTCTGACTAT 
GAAGACACAG ACACAGAAGG CGGGGCCTAC ACTGATCAAG AACTAGATGA AACTCTTAAT 
GATGAGGTTG GGACTCCACC GGAGTCTGCC ATTACACGGT CCTCTGAGCC TGTAAGAGAG 
GACTCCTCTG GAATGCATCA TGAAAACCAA ACATATCCTC CTTACTCACC ACAAGCGCAG 
CCACAACCAA TTCATAGAAT AGACTCCCCT GGATTTAAGC CAGCCTCTCA ACAGAAAGCA 
GAAGCTTCAT CTCCAGTCCC TTACCTTTCG CCTGAAACAA ACCCAGCATC ATCAACCTCT 
GCTGTTAATC ATAATGTAAA TTTAACTAAT GTCAGACTGG AGGAGCCCAC CCCAGCTCCT 
TCCACCTCTT ACTCACCACA AGCTGATTCT TTAAGAACAC CAAGTACTGA GGCAGCTCAC 
ATAATGCTAA GAGATCAAGA ACCATCATTG TCGTCGCATG TAGATCCAAC AAAGGTGTAT 
AGAAAGGATC CATATCCCGA GGAAATGATG AGGCAGAACC ATGTTTTGAA ACAGCCAGCC 
GTTAGTCACC CAGGGCACAG GCCAGACAAA GAGCCTAATC TGACCTATGA ACCCCAACTC 
CCATACGTAG AGAAACAAGC CAGCAGAGAC CTCGAGCAGC CCACATACAG ATACGAGTCC 
TCAAGCTATA CGGACCAGTT TTCTCGAAAC TATGAACATC GTCTGCGATA CGAAGATCGC 
GTCCCCATGT ATGAAGAACA GTGGTCATAT TATGATGACA AACAGCCCTA CCCATCTCGG 
CCACCTTTTG ATAATCAGCA CTCTCAAGAC CTTGACTCCA GACAGCATCC CGAAGAGTCC 
TCAGAACGAG GGTACTTTCC ACGTTTTGAA GAGCCAGCCC CTCTGTCTTA CGACAGCAGA 
CCACGTTACG AACAGGCACC TAGAGCATCC GCCCTGCGGC ACGAAGAGCA GCCAGCTCCT 
GGGTATGACA CACATGGTAG ACTCAGACCG GAAGCCCAGC CCCACCCTTC AGCAGGGCCC 
AAGCCCGCAG AGTCCAAGCA GTATTTTGAG CAATATTCAC GCAGTTACGA GCAAGTACCA 
CCCCAAGGAT TTACCTCTAG AGCAGGTCAT TTTGAGCCTC TCCATGGTGC TGCAGCTGTC 
CCTCCGCTGA TACCTTCATC TCAGCATAAG CCAGAAGCTC TGCCTTCAAA CACCAAACCA 
CTGCCTCCAC CCCCAACTCA AACCGAAGAA GAGGAAGATC CAGCAATGAA GCCACAGTCT 
GTACTCACCA GAGTTAAGAT GTTTGAAAAC AAAAGATCTG CATCCTTAGA GACCAAGAAG 
GATGTAAATG ACACTGGCAG TTTTAAGCCT CCAGAAGTAG CATCTAAACC TTCAGGTGCT 
CCCATCATTG GTCCCAAACC CACTTCTCAG AATCAGTTCA GTGAACATGA CAAAACTCTG 
TACAGGATCC CAGAACCTCA AAAACCTCAA CTGAAGCCAC CTGAAGATAT CGTTCGGTCC 
AATCATTATG ACCCTGAAGA AGATGAAGAA TATTATCGAA AACAGCTGTC ATACTTTGAC 
CGAAGAAGTT TTGAGAATAA GCCTCCTGCA CACATTGCCG CCAGCCATCT CTCCGAGCCT 
GCAAAGCCAG CTCATTCTCA GAATCAATCA AATTTTTCTA GTTATTCTTC AAAGGGAAAG 
CCTCCTGAAG CTGATGGTGT GGATAGATCA TTTGGCGAGA AACGCTATGA ACCCATCCAG 
GCCACTCCCC CTCCTCCTCC ATTGCCCTCG CAGTATGCCC AGCCATCTCA GCCTGTCACC 
AGCGCGTCTC TCCACATACA TTCTAAGGGA GCACATGGTG AAGGTAATTC AGTGTCATTG 
GATTTTCAGA ATTCCTTAGT GTCCAAACCA GACCCACCTC CATCTCAGAA TAAGCCAGCA 
ACTTTCAGAC CACCAAACCG AGAAGATACT GCTCAGCCAG CTTTCTATCC CCAGAAAAGT 
TTTCCAGATA AAGCCCCAGT TAATGGAACT GAACAGACTC AGAAAACAGT CACTCCAGCA 
TACAATCGAT TCACACCAAA ACCATATACA AGTTCTGCCC GACCATTTGA ACGCAAGTTT 
GAAAGTCCTA AATTCAATCA CAATCTTCTG CCAAGTGAAA CTGCACATAA ACCTGAATTG 
TCTTCAAAAA CTCCCACTTC TCCAAAAACT CTTGTGAAAT CGCACAGTTT GGCACAGCCT 
CCTGAGTTTG ACAGTGGAGT TGAAACTTTC TCTATCCATG CAGAGAAGCC TAAATATCAA 
ATAAATAATA TCAGCACAGT GCCTAAAGCT ATTCCTGTGA GTCCTTCAGC TGTGGAAGAG 
GATGAAGATG AAGATGGTCA TACTGTGGTG GCCACAGCCC GAGGCATATT TAACAGCAAT 
GGGGGCGTGC TGAGTTCCAT AGAAACTGGC GTTAGTATAA TTATCCCTCA AGGAGCCATT 
CCCGAAGGAG TTGAGCAGGA AATCTATTTC AAGGTCTGCC GGGACAACAG CATCCTTCCA 
CCTTTAGATA AAGAGAAAGG TGAAACACTG CTGAGTCCTT TGGTGATGTG TGGTCCCCAT 
GGCCTCAAGT TCCTGAAGCC TGTGGAGCTG CGCTTACCAC ACTGTGCGTC CATGACTCCT 
GACGGTTGGT CTTTTGCTCT AAAATCATCC GACTCCTCGT CGGGTGATCC TAAAACCTGG 
CAAAACAAGT GTCTTCCCGG AGATCCAAAT TATCTCGTTG GAGCAAACTG TGTTTCTGTC 
CTTATTGACC ACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016783329.1
CDS	16..5313
Translation	MDCHSKSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQKAEASSPVPYLSPETNPASSTSAVNHNVNLTNVRLEEPTPAPSTSYSPQADSLRTPSTEAAHIMLRDQEPSLSSHVDPTKVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQPAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPELSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_016927840.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes tight junction protein 1 (TJP1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016927840.1

ORF Insert Sequence:

ATGGATTGCC ACAGTAAGAG CACAGCAATG GAGGAAACAG CTATATGGGA ACAACATACA 
GTGACGCTTC ACAGGGCTCC TGGATTTGGG TTTGGAATTG CAATATCTGG TGGACGAGAT 
AATCCTCATT TTCAGAGTGG GGAAACGTCA ATAGTGATTT CAGATGTGCT GAAAGGAGGA 
CCAGCTGAAG GACAGCTACA GGAAAATGAC CGAGTTGCAA TGGTTAACGG AGTTTCAATG 
GATAATGTTG AACATGCTTT TGCTGTTCAG CAACTAAGGA AAAGTGGGAA AAATGCAAAA 
ATTACAATTA GAAGGAAGAA GAAAGTTCAA ATACCAGTAA GTCGTCCTGA TCCTGAACCA 
GTATCTGATA ATGAAGAAGA TAGTTATGAT GAGGAAATAC ATGATCCAAG AAGTGGCCGG 
AGTGGTGTGG TTAACAGAAG GAGTGAGAAG ATTTGGCCGA GGGATAGAAG TGCAAGTAGA 
GAGAGGAGCT TGTCCCCGCG GTCAGACAGG CGGTCAGTGG CTTCCAGCCA GCCCGCTAAA 
CCTACTAAAG TCACACTGGT GAAATCCCGG AAAAATGAAG AATATGGTCT TCGATTGGCA 
AGCCATATAT TTGTTAAGGA AATTTCACAA GATAGTTTGG CAGCAAGAGA TGGCAATATT 
CAAGAAGGTG ATGTTGTATT GAAGATAAAT GGTACTGTGA CAGAAAATAT GTCATTGACA 
GATGCAAAGA CATTGATAGA AAGGTCTAAA GGCAAATTAA AAATGGTAGT TCAAAGAGAT 
GAACGGGCTA CGCTATTGAA TGTCCCTGAT CTTTCTGACA GCATCCACTC TGCTAATGCC 
TCTGAGAGAG ACGACATTTC AGAAATTCAG TCACTGGCAT CAGATCATTC TGGTCGATCA 
CACGATAGGC CTCCCCGCCG CAGCCGGTCA CGATCTCCTG ACCAGCGGTC AGAGCCTTCT 
GATCATTCCA GGCACTCGCC ACAGCAGCCA AGCAATGGCA GTCTCCGGAG TAGAGATGAA 
GAGAGAATTT CTAAACCTGG GGCTGTCTCA ACTCCTGTAA AGCATGCTGA TGATCACACA 
CCTAAAACAG TGGAAGAAGT TACAGTTGAA AGAAATGAGA AACAAACACC TTCTCTTCCA 
GAACCAAAGC CTGTGTATGC CCAAGTTGGG CAGCCAGATG TGGATTTACC TGTCAGTCCA 
TCTGATGGTG TCCTACCTAA TTCAACTCAT GAAGATGGGA TTCTTCGGCC CAGCATGAAA 
TTGGTAAAAT TCAGAAAAGG AGATAGTGTG GGTTTGCGGC TGGCTGGTGG AAATGATGTT 
GGAATATTTG TAGCTGGCGT TCTAGAAGAT AGCCCTGCAG CCAAGGAAGG CTTAGAGGAA 
GGTGATCAAA TTCTCAGGGT AAACAACGTA GATTTTACAA ATATCATAAG AGAAGAAGCC 
GTCCTTTTCC TGCTTGACCT CCCTAAAGGA GAAGAAGTGA CCATATTGGC TCAGAAGAAG 
AAGGATGTTT ATCGTCGCAT TGTAGAATCA GATGTAGGAG ATTCTTTCTA TATTAGAACC 
CATTTTGAAT ATGAAAAGGA ATCTCCCTAT GGACTTAGTT TTAACAAAGG AGAGGTGTTC 
CGTGTTGTGG ATACCTTGTA CAATGGAAAA CTGGGCTCTT GGCTTGCTAT TCGAATTGGT 
AAAAATCATA AGGAGGTAGA ACGAGGCATC ATCCCTAATA AGAACAGAGC TGAGCAGCTA 
GCCAGTGTAC AGTATACACT TCCAAAAACA GCAGGCGGAG ACCGTGCTGA CTTCTGGAGA 
TTCAGAGGTC TTCGCAGCTC CAAGAGAAAT CTTCGAAAAA GCAGAGAGGA TTTGTCCGCT 
CAGCCTGTTC AAACAAAGTT TCCAGCTTAT GAAAGAGTGG TTCTTCGAGA AGCTGGATTT 
CTGAGGCCTG TAACCATTTT TGGACCAATA GCTGATGTTG CCAGAGAAAA GCTGGCAAGA 
GAAGAACCAG ATATTTATCA AATTGCAAAG AGTGAACCAC GAGACGCTGG AACTGACCAA 
CGTAGCTCTG GCATTATTCG CCTGCATACA ATAAAGCAAA TCATAGATCA AGACAAACAT 
GCTTTATTAG ATGTAACACC AAATGCAGTT GATCGTCTTA ACTATGCCCA GTGGTATCCA 
ATTGTTGTAT TTCTTAACCC TGATTCTAAG CAAGGAGTAA AAACAATGAG AATGAGGTTA 
TGTCCAGAAT CTCGGAAAAG TGCCAGGAAG TTATATGAGC GATCTCATAA ACTTCGTAAA 
AATAATCACC ATCTTTTTAC AACTACAATT AACTTAAATT CAATGAATGA TGGTTGGTAT 
GGTGCGCTGA AAGAAGCAAT TCAACAACAG CAAAACCAGC TGGTATGGGT TTCCGAGGGA 
AAGGCGGATG GTGCTACAAG TGATGACCTT GATTTGCATG ATGATCGTCT GTCCTACCTG 
TCAGCTCCAG GTAGTGAATA CTCAATGTAT AGCACGGACA GTAGACACAC TTCTGACTAT 
GAAGACACAG ACACAGAAGG CGGGGCCTAC ACTGATCAAG AACTAGATGA AACTCTTAAT 
GATGAGGTTG GGACTCCACC GGAGTCTGCC ATTACACGGT CCTCTGAGCC TGTAAGAGAG 
GACTCCTCTG GAATGCATCA TGAAAACCAA ACATATCCTC CTTACTCACC ACAAGCGCAG 
CCACAACCAA TTCATAGAAT AGACTCCCCT GGATTTAAGC CAGCCTCTCA ACAGAAAGCA 
GAAGCTTCAT CTCCAGTCCC TTACCTTTCG CCTGAAACAA ACCCAGCATC ATCAACCTCT 
GCTGTTAATC ATAATGTAAA TTTAACTAAT GTCAGACTGG AGGAGCCCAC CCCAGCTCCT 
TCCACCTCTT ACTCACCACA AGCTGATTCT TTAAGAACAC CAAGTACTGA GGCAGCTCAC 
ATAATGCTAA GAGATCAAGA ACCATCATTG TCGTCGCATG TAGATCCAAC AAAGGTGTAT 
AGAAAGGATC CATATCCCGA GGAAATGATG AGGCAGAACC ATGTTTTGAA ACAGCCAGCC 
GTTAGTCACC CAGGGCACAG GCCAGACAAA GAGCCTAATC TGACCTATGA ACCCCAACTC 
CCATACGTAG AGAAACAAGC CAGCAGAGAC CTCGAGCAGC CCACATACAG ATACGAGTCC 
TCAAGCTATA CGGACCAGTT TTCTCGAAAC TATGAACATC GTCTGCGATA CGAAGATCGC 
GTCCCCATGT ATGAAGAACA GTGGTCATAT TATGATGACA AACAGCCCTA CCCATCTCGG 
CCACCTTTTG ATAATCAGCA CTCTCAAGAC CTTGACTCCA GACAGCATCC CGAAGAGTCC 
TCAGAACGAG GGTACTTTCC ACGTTTTGAA GAGCCAGCCC CTCTGTCTTA CGACAGCAGA 
CCACGTTACG AACAGGCACC TAGAGCATCC GCCCTGCGGC ACGAAGAGCA GCCAGCTCCT 
GGGTATGACA CACATGGTAG ACTCAGACCG GAAGCCCAGC CCCACCCTTC AGCAGGGCCC 
AAGCCCGCAG AGTCCAAGCA GTATTTTGAG CAATATTCAC GCAGTTACGA GCAAGTACCA 
CCCCAAGGAT TTACCTCTAG AGCAGGTCAT TTTGAGCCTC TCCATGGTGC TGCAGCTGTC 
CCTCCGCTGA TACCTTCATC TCAGCATAAG CCAGAAGCTC TGCCTTCAAA CACCAAACCA 
CTGCCTCCAC CCCCAACTCA AACCGAAGAA GAGGAAGATC CAGCAATGAA GCCACAGTCT 
GTACTCACCA GAGTTAAGAT GTTTGAAAAC AAAAGATCTG CATCCTTAGA GACCAAGAAG 
GATGTAAATG ACACTGGCAG TTTTAAGCCT CCAGAAGTAG CATCTAAACC TTCAGGTGCT 
CCCATCATTG GTCCCAAACC CACTTCTCAG AATCAGTTCA GTGAACATGA CAAAACTCTG 
TACAGGATCC CAGAACCTCA AAAACCTCAA CTGAAGCCAC CTGAAGATAT CGTTCGGTCC 
AATCATTATG ACCCTGAAGA AGATGAAGAA TATTATCGAA AACAGCTGTC ATACTTTGAC 
CGAAGAAGTT TTGAGAATAA GCCTCCTGCA CACATTGCCG CCAGCCATCT CTCCGAGCCT 
GCAAAGCCAG CTCATTCTCA GAATCAATCA AATTTTTCTA GTTATTCTTC AAAGGGAAAG 
CCTCCTGAAG CTGATGGTGT GGATAGATCA TTTGGCGAGA AACGCTATGA ACCCATCCAG 
GCCACTCCCC CTCCTCCTCC ATTGCCCTCG CAGTATGCCC AGCCATCTCA GCCTGTCACC 
AGCGCGTCTC TCCACATACA TTCTAAGGGA GCACATGGTG AAGGTAATTC AGTGTCATTG 
GATTTTCAGA ATTCCTTAGT GTCCAAACCA GACCCACCTC CATCTCAGAA TAAGCCAGCA 
ACTTTCAGAC CACCAAACCG AGAAGATACT GCTCAGCCAG CTTTCTATCC CCAGAAAAGT 
TTTCCAGATA AAGCCCCAGT TAATGGAACT GAACAGACTC AGAAAACAGT CACTCCAGCA 
TACAATCGAT TCACACCAAA ACCATATACA AGTTCTGCCC GACCATTTGA ACGCAAGTTT 
GAAAGTCCTA AATTCAATCA CAATCTTCTG CCAAGTGAAA CTGCACATAA ACCTGAATTG 
TCTTCAAAAA CTCCCACTTC TCCAAAAACT CTTGTGAAAT CGCACAGTTT GGCACAGCCT 
CCTGAGTTTG ACAGTGGAGT TGAAACTTTC TCTATCCATG CAGAGAAGCC TAAATATCAA 
ATAAATAATA TCAGCACAGT GCCTAAAGCT ATTCCTGTGA GTCCTTCAGC TGTGGAAGAG 
GATGAAGATG AAGATGGTCA TACTGTGGTG GCCACAGCCC GAGGCATATT TAACAGCAAT 
GGGGGCGTGC TGAGTTCCAT AGAAACTGGC GTTAGTATAA TTATCCCTCA AGGAGCCATT 
CCCGAAGGAG TTGAGCAGGA AATCTATTTC AAGGTCTGCC GGGACAACAG CATCCTTCCA 
CCTTTAGATA AAGAGAAAGG TGAAACACTG CTGAGTCCTT TGGTGATGTG TGGTCCCCAT 
GGCCTCAAGT TCCTGAAGCC TGTGGAGCTG CGCTTACCAC ACTGTGCGTC CATGACTCCT 
GACGGTTGGT CTTTTGCTCT AAAATCATCC GACTCCTCGT CGGGTGATCC TAAAACCTGG 
CAAAACAAGT GTCTTCCCGG AGATCCAAAT TATCTCGTTG GAGCAAACTG TGTTTCTGTC 
CTTATTGACC ACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_001163120.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_001163120.2 Pan troglodytes tight junction protein 1 (TJP1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OPc69383
Clone ID Related Accession (Same CDS sequence)	XM_001163120.3
Accession Version	XM_001163120.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5247bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	tight junction protein ZO-1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006482.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_001163120.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGGT TTGGAATTGC AATATCTGGT 
GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG 
AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA 
GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT 
CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA 
AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT 
GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG 
CCCGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT 
CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT 
GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT 
GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA 
GAGCCTTCTG ATCATTCCAG GCACTCGCCA CAGCAGCCAA GCAATGGCAG TCTCCGGAGT 
AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT 
GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT 
TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AGCCAGATGT GGATTTACCT 
GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC 
AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA 
AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA 
GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT 
CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC 
TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT 
TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA 
GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA 
GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG 
TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA 
ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATATGAGCG ATCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT 
TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA 
CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA 
CAGAAAGCAG AAGCTTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA 
TCAACCTCTG CTGTTAATCA TAATGTAAAT TTAACTAATG TCAGACTGGA GGAGCCCACC 
CCAGCTCCTT CCACCTCTTA CTCACCACAA GCTGATTCTT TAAGAACACC AAGTACTGAG 
GCAGCTCACA TAATGCTAAG AGATCAAGAA CCATCATTGT CGTCGCATGT AGATCCAACA 
AAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA 
CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA 
CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA 
TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC 
GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC 
GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC 
GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG 
CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA 
GCAGGGCCCA AGCCCGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG 
CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT 
GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC 
ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG 
CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG 
ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT 
TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAGTTCAG TGAACATGAC 
AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATC 
GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA 
TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC 
TCCGAGCCTG CAAAGCCAGC TCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA 
AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA 
CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG 
CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA 
GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT 
AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGCCAGC TTTCTATCCC 
CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC 
ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA 
CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA 
CCTGAATTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG 
GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT 
AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT 
GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT 
AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGCG TTAGTATAAT TATCCCTCAA 
GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC 
ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT 
GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGATCCT 
AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT 
GTTTCTGTCC TTATTGACCA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001163120.2
CDS	476..5722
Translation	MSARAAAAKSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQKAEASSPVPYLSPETNPASSTSAVNHNVNLTNVRLEEPTPAPSTSYSPQADSLRTPSTEAAHIMLRDQEPSLSSHVDPTKVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQPAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPELSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_001163120.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes tight junction protein 1 (TJP1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001163120.3

ORF Insert Sequence:

ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGGT TTGGAATTGC AATATCTGGT 
GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG 
AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA 
GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT 
CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA 
AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT 
GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG 
CCCGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT 
CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT 
GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT 
GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA 
GAGCCTTCTG ATCATTCCAG GCACTCGCCA CAGCAGCCAA GCAATGGCAG TCTCCGGAGT 
AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT 
GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT 
TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AGCCAGATGT GGATTTACCT 
GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC 
AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA 
AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA 
GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT 
CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC 
TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT 
TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA 
GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA 
GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG 
TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA 
ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATATGAGCG ATCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT 
TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA 
CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA 
CAGAAAGCAG AAGCTTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA 
TCAACCTCTG CTGTTAATCA TAATGTAAAT TTAACTAATG TCAGACTGGA GGAGCCCACC 
CCAGCTCCTT CCACCTCTTA CTCACCACAA GCTGATTCTT TAAGAACACC AAGTACTGAG 
GCAGCTCACA TAATGCTAAG AGATCAAGAA CCATCATTGT CGTCGCATGT AGATCCAACA 
AAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA 
CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA 
CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA 
TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC 
GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC 
GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC 
GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG 
CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA 
GCAGGGCCCA AGCCCGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG 
CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT 
GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC 
ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG 
CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG 
ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT 
TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAGTTCAG TGAACATGAC 
AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATC 
GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA 
TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC 
TCCGAGCCTG CAAAGCCAGC TCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA 
AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA 
CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG 
CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA 
GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT 
AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGCCAGC TTTCTATCCC 
CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC 
ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA 
CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA 
CCTGAATTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG 
GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT 
AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT 
GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT 
AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGCG TTAGTATAAT TATCCCTCAA 
GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC 
ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT 
GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGATCCT 
AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT 
GTTTCTGTCC TTATTGACCA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_016927841.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016783330.1 Pan troglodytes tight junction protein 1 (TJP1), transcript variant X11, mRNA.

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CloneID	OPc69384
Clone ID Related Accession (Same CDS sequence)	XM_016927841.1
Accession Version	XM_016927841.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5301bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	tight junction protein ZO-1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006482.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTCT GTGAGCTCAC TAGCACAGCA ATGGAGGAAA CAGCTATATG GGAACAACAT 
ACAGTGACGC TTCACAGGGC TCCTGGATTT GGGTTTGGAA TTGCAATATC TGGTGGACGA 
GATAATCCTC ATTTTCAGAG TGGGGAAACG TCAATAGTGA TTTCAGATGT GCTGAAAGGA 
GGACCAGCTG AAGGACAGCT ACAGGAAAAT GACCGAGTTG CAATGGTTAA CGGAGTTTCA 
ATGGATAATG TTGAACATGC TTTTGCTGTT CAGCAACTAA GGAAAAGTGG GAAAAATGCA 
AAAATTACAA TTAGAAGGAA GAAGAAAGTT CAAATACCAG TAAGTCGTCC TGATCCTGAA 
CCAGTATCTG ATAATGAAGA AGATAGTTAT GATGAGGAAA TACATGATCC AAGAAGTGGC 
CGGAGTGGTG TGGTTAACAG AAGGAGTGAG AAGATTTGGC CGAGGGATAG AAGTGCAAGT 
AGAGAGAGGA GCTTGTCCCC GCGGTCAGAC AGGCGGTCAG TGGCTTCCAG CCAGCCCGCT 
AAACCTACTA AAGTCACACT GGTGAAATCC CGGAAAAATG AAGAATATGG TCTTCGATTG 
GCAAGCCATA TATTTGTTAA GGAAATTTCA CAAGATAGTT TGGCAGCAAG AGATGGCAAT 
ATTCAAGAAG GTGATGTTGT ATTGAAGATA AATGGTACTG TGACAGAAAA TATGTCATTG 
ACAGATGCAA AGACATTGAT AGAAAGGTCT AAAGGCAAAT TAAAAATGGT AGTTCAAAGA 
GATGAACGGG CTACGCTATT GAATGTCCCT GATCTTTCTG ACAGCATCCA CTCTGCTAAT 
GCCTCTGAGA GAGACGACAT TTCAGAAATT CAGTCACTGG CATCAGATCA TTCTGGTCGA 
TCACACGATA GGCCTCCCCG CCGCAGCCGG TCACGATCTC CTGACCAGCG GTCAGAGCCT 
TCTGATCATT CCAGGCACTC GCCACAGCAG CCAAGCAATG GCAGTCTCCG GAGTAGAGAT 
GAAGAGAGAA TTTCTAAACC TGGGGCTGTC TCAACTCCTG TAAAGCATGC TGATGATCAC 
ACACCTAAAA CAGTGGAAGA AGTTACAGTT GAAAGAAATG AGAAACAAAC ACCTTCTCTT 
CCAGAACCAA AGCCTGTGTA TGCCCAAGTT GGGCAGCCAG ATGTGGATTT ACCTGTCAGT 
CCATCTGATG GTGTCCTACC TAATTCAACT CATGAAGATG GGATTCTTCG GCCCAGCATG 
AAATTGGTAA AATTCAGAAA AGGAGATAGT GTGGGTTTGC GGCTGGCTGG TGGAAATGAT 
GTTGGAATAT TTGTAGCTGG CGTTCTAGAA GATAGCCCTG CAGCCAAGGA AGGCTTAGAG 
GAAGGTGATC AAATTCTCAG GGTAAACAAC GTAGATTTTA CAAATATCAT AAGAGAAGAA 
GCCGTCCTTT TCCTGCTTGA CCTCCCTAAA GGAGAAGAAG TGACCATATT GGCTCAGAAG 
AAGAAGGATG TTTATCGTCG CATTGTAGAA TCAGATGTAG GAGATTCTTT CTATATTAGA 
ACCCATTTTG AATATGAAAA GGAATCTCCC TATGGACTTA GTTTTAACAA AGGAGAGGTG 
TTCCGTGTTG TGGATACCTT GTACAATGGA AAACTGGGCT CTTGGCTTGC TATTCGAATT 
GGTAAAAATC ATAAGGAGGT AGAACGAGGC ATCATCCCTA ATAAGAACAG AGCTGAGCAG 
CTAGCCAGTG TACAGTATAC ACTTCCAAAA ACAGCAGGCG GAGACCGTGC TGACTTCTGG 
AGATTCAGAG GTCTTCGCAG CTCCAAGAGA AATCTTCGAA AAAGCAGAGA GGATTTGTCC 
GCTCAGCCTG TTCAAACAAA GTTTCCAGCT TATGAAAGAG TGGTTCTTCG AGAAGCTGGA 
TTTCTGAGGC CTGTAACCAT TTTTGGACCA ATAGCTGATG TTGCCAGAGA AAAGCTGGCA 
AGAGAAGAAC CAGATATTTA TCAAATTGCA AAGAGTGAAC CACGAGACGC TGGAACTGAC 
CAACGTAGCT CTGGCATTAT TCGCCTGCAT ACAATAAAGC AAATCATAGA TCAAGACAAA 
CATGCTTTAT TAGATGTAAC ACCAAATGCA GTTGATCGTC TTAACTATGC CCAGTGGTAT 
CCAATTGTTG TATTTCTTAA CCCTGATTCT AAGCAAGGAG TAAAAACAAT GAGAATGAGG 
TTATGTCCAG AATCTCGGAA AAGTGCCAGG AAGTTATATG AGCGATCTCA TAAACTTCGT 
AAAAATAATC ACCATCTTTT TACAACTACA ATTAACTTAA ATTCAATGAA TGATGGTTGG 
TATGGTGCGC TGAAAGAAGC AATTCAACAA CAGCAAAACC AGCTGGTATG GGTTTCCGAG 
GGAAAGGCGG ATGGTGCTAC AAGTGATGAC CTTGATTTGC ATGATGATCG TCTGTCCTAC 
CTGTCAGCTC CAGGTAGTGA ATACTCAATG TATAGCACGG ACAGTAGACA CACTTCTGAC 
TATGAAGACA CAGACACAGA AGGCGGGGCC TACACTGATC AAGAACTAGA TGAAACTCTT 
AATGATGAGG TTGGGACTCC ACCGGAGTCT GCCATTACAC GGTCCTCTGA GCCTGTAAGA 
GAGGACTCCT CTGGAATGCA TCATGAAAAC CAAACATATC CTCCTTACTC ACCACAAGCG 
CAGCCACAAC CAATTCATAG AATAGACTCC CCTGGATTTA AGCCAGCCTC TCAACAGAAA 
GCAGAAGCTT CATCTCCAGT CCCTTACCTT TCGCCTGAAA CAAACCCAGC ATCATCAACC 
TCTGCTGTTA ATCATAATGT AAATTTAACT AATGTCAGAC TGGAGGAGCC CACCCCAGCT 
CCTTCCACCT CTTACTCACC ACAAGCTGAT TCTTTAAGAA CACCAAGTAC TGAGGCAGCT 
CACATAATGC TAAGAGATCA AGAACCATCA TTGTCGTCGC ATGTAGATCC AACAAAGGTG 
TATAGAAAGG ATCCATATCC CGAGGAAATG ATGAGGCAGA ACCATGTTTT GAAACAGCCA 
GCCGTTAGTC ACCCAGGGCA CAGGCCAGAC AAAGAGCCTA ATCTGACCTA TGAACCCCAA 
CTCCCATACG TAGAGAAACA AGCCAGCAGA GACCTCGAGC AGCCCACATA CAGATACGAG 
TCCTCAAGCT ATACGGACCA GTTTTCTCGA AACTATGAAC ATCGTCTGCG ATACGAAGAT 
CGCGTCCCCA TGTATGAAGA ACAGTGGTCA TATTATGATG ACAAACAGCC CTACCCATCT 
CGGCCACCTT TTGATAATCA GCACTCTCAA GACCTTGACT CCAGACAGCA TCCCGAAGAG 
TCCTCAGAAC GAGGGTACTT TCCACGTTTT GAAGAGCCAG CCCCTCTGTC TTACGACAGC 
AGACCACGTT ACGAACAGGC ACCTAGAGCA TCCGCCCTGC GGCACGAAGA GCAGCCAGCT 
CCTGGGTATG ACACACATGG TAGACTCAGA CCGGAAGCCC AGCCCCACCC TTCAGCAGGG 
CCCAAGCCCG CAGAGTCCAA GCAGTATTTT GAGCAATATT CACGCAGTTA CGAGCAAGTA 
CCACCCCAAG GATTTACCTC TAGAGCAGGT CATTTTGAGC CTCTCCATGG TGCTGCAGCT 
GTCCCTCCGC TGATACCTTC ATCTCAGCAT AAGCCAGAAG CTCTGCCTTC AAACACCAAA 
CCACTGCCTC CACCCCCAAC TCAAACCGAA GAAGAGGAAG ATCCAGCAAT GAAGCCACAG 
TCTGTACTCA CCAGAGTTAA GATGTTTGAA AACAAAAGAT CTGCATCCTT AGAGACCAAG 
AAGGATGTAA ATGACACTGG CAGTTTTAAG CCTCCAGAAG TAGCATCTAA ACCTTCAGGT 
GCTCCCATCA TTGGTCCCAA ACCCACTTCT CAGAATCAGT TCAGTGAACA TGACAAAACT 
CTGTACAGGA TCCCAGAACC TCAAAAACCT CAACTGAAGC CACCTGAAGA TATCGTTCGG 
TCCAATCATT ATGACCCTGA AGAAGATGAA GAATATTATC GAAAACAGCT GTCATACTTT 
GACCGAAGAA GTTTTGAGAA TAAGCCTCCT GCACACATTG CCGCCAGCCA TCTCTCCGAG 
CCTGCAAAGC CAGCTCATTC TCAGAATCAA TCAAATTTTT CTAGTTATTC TTCAAAGGGA 
AAGCCTCCTG AAGCTGATGG TGTGGATAGA TCATTTGGCG AGAAACGCTA TGAACCCATC 
CAGGCCACTC CCCCTCCTCC TCCATTGCCC TCGCAGTATG CCCAGCCATC TCAGCCTGTC 
ACCAGCGCGT CTCTCCACAT ACATTCTAAG GGAGCACATG GTGAAGGTAA TTCAGTGTCA 
TTGGATTTTC AGAATTCCTT AGTGTCCAAA CCAGACCCAC CTCCATCTCA GAATAAGCCA 
GCAACTTTCA GACCACCAAA CCGAGAAGAT ACTGCTCAGC CAGCTTTCTA TCCCCAGAAA 
AGTTTTCCAG ATAAAGCCCC AGTTAATGGA ACTGAACAGA CTCAGAAAAC AGTCACTCCA 
GCATACAATC GATTCACACC AAAACCATAT ACAAGTTCTG CCCGACCATT TGAACGCAAG 
TTTGAAAGTC CTAAATTCAA TCACAATCTT CTGCCAAGTG AAACTGCACA TAAACCTGAA 
TTGTCTTCAA AAACTCCCAC TTCTCCAAAA ACTCTTGTGA AATCGCACAG TTTGGCACAG 
CCTCCTGAGT TTGACAGTGG AGTTGAAACT TTCTCTATCC ATGCAGAGAA GCCTAAATAT 
CAAATAAATA ATATCAGCAC AGTGCCTAAA GCTATTCCTG TGAGTCCTTC AGCTGTGGAA 
GAGGATGAAG ATGAAGATGG TCATACTGTG GTGGCCACAG CCCGAGGCAT ATTTAACAGC 
AATGGGGGCG TGCTGAGTTC CATAGAAACT GGCGTTAGTA TAATTATCCC TCAAGGAGCC 
ATTCCCGAAG GAGTTGAGCA GGAAATCTAT TTCAAGGTCT GCCGGGACAA CAGCATCCTT 
CCACCTTTAG ATAAAGAGAA AGGTGAAACA CTGCTGAGTC CTTTGGTGAT GTGTGGTCCC 
CATGGCCTCA AGTTCCTGAA GCCTGTGGAG CTGCGCTTAC CACACTGTGC GTCCATGACT 
CCTGACGGTT GGTCTTTTGC TCTAAAATCA TCCGACTCCT CGTCGGGTGA TCCTAAAACC 
TGGCAAAACA AGTGTCTTCC CGGAGATCCA AATTATCTCG TTGGAGCAAA CTGTGTTTCT 
GTCCTTATTG ACCACTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016783330.1
CDS	419..5719
Translation	MDFCELTSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQKAEASSPVPYLSPETNPASSTSAVNHNVNLTNVRLEEPTPAPSTSYSPQADSLRTPSTEAAHIMLRDQEPSLSSHVDPTKVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQPAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPELSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_016927841.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes tight junction protein 1 (TJP1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016927841.1

ORF Insert Sequence:

ATGGATTTCT GTGAGCTCAC TAGCACAGCA ATGGAGGAAA CAGCTATATG GGAACAACAT 
ACAGTGACGC TTCACAGGGC TCCTGGATTT GGGTTTGGAA TTGCAATATC TGGTGGACGA 
GATAATCCTC ATTTTCAGAG TGGGGAAACG TCAATAGTGA TTTCAGATGT GCTGAAAGGA 
GGACCAGCTG AAGGACAGCT ACAGGAAAAT GACCGAGTTG CAATGGTTAA CGGAGTTTCA 
ATGGATAATG TTGAACATGC TTTTGCTGTT CAGCAACTAA GGAAAAGTGG GAAAAATGCA 
AAAATTACAA TTAGAAGGAA GAAGAAAGTT CAAATACCAG TAAGTCGTCC TGATCCTGAA 
CCAGTATCTG ATAATGAAGA AGATAGTTAT GATGAGGAAA TACATGATCC AAGAAGTGGC 
CGGAGTGGTG TGGTTAACAG AAGGAGTGAG AAGATTTGGC CGAGGGATAG AAGTGCAAGT 
AGAGAGAGGA GCTTGTCCCC GCGGTCAGAC AGGCGGTCAG TGGCTTCCAG CCAGCCCGCT 
AAACCTACTA AAGTCACACT GGTGAAATCC CGGAAAAATG AAGAATATGG TCTTCGATTG 
GCAAGCCATA TATTTGTTAA GGAAATTTCA CAAGATAGTT TGGCAGCAAG AGATGGCAAT 
ATTCAAGAAG GTGATGTTGT ATTGAAGATA AATGGTACTG TGACAGAAAA TATGTCATTG 
ACAGATGCAA AGACATTGAT AGAAAGGTCT AAAGGCAAAT TAAAAATGGT AGTTCAAAGA 
GATGAACGGG CTACGCTATT GAATGTCCCT GATCTTTCTG ACAGCATCCA CTCTGCTAAT 
GCCTCTGAGA GAGACGACAT TTCAGAAATT CAGTCACTGG CATCAGATCA TTCTGGTCGA 
TCACACGATA GGCCTCCCCG CCGCAGCCGG TCACGATCTC CTGACCAGCG GTCAGAGCCT 
TCTGATCATT CCAGGCACTC GCCACAGCAG CCAAGCAATG GCAGTCTCCG GAGTAGAGAT 
GAAGAGAGAA TTTCTAAACC TGGGGCTGTC TCAACTCCTG TAAAGCATGC TGATGATCAC 
ACACCTAAAA CAGTGGAAGA AGTTACAGTT GAAAGAAATG AGAAACAAAC ACCTTCTCTT 
CCAGAACCAA AGCCTGTGTA TGCCCAAGTT GGGCAGCCAG ATGTGGATTT ACCTGTCAGT 
CCATCTGATG GTGTCCTACC TAATTCAACT CATGAAGATG GGATTCTTCG GCCCAGCATG 
AAATTGGTAA AATTCAGAAA AGGAGATAGT GTGGGTTTGC GGCTGGCTGG TGGAAATGAT 
GTTGGAATAT TTGTAGCTGG CGTTCTAGAA GATAGCCCTG CAGCCAAGGA AGGCTTAGAG 
GAAGGTGATC AAATTCTCAG GGTAAACAAC GTAGATTTTA CAAATATCAT AAGAGAAGAA 
GCCGTCCTTT TCCTGCTTGA CCTCCCTAAA GGAGAAGAAG TGACCATATT GGCTCAGAAG 
AAGAAGGATG TTTATCGTCG CATTGTAGAA TCAGATGTAG GAGATTCTTT CTATATTAGA 
ACCCATTTTG AATATGAAAA GGAATCTCCC TATGGACTTA GTTTTAACAA AGGAGAGGTG 
TTCCGTGTTG TGGATACCTT GTACAATGGA AAACTGGGCT CTTGGCTTGC TATTCGAATT 
GGTAAAAATC ATAAGGAGGT AGAACGAGGC ATCATCCCTA ATAAGAACAG AGCTGAGCAG 
CTAGCCAGTG TACAGTATAC ACTTCCAAAA ACAGCAGGCG GAGACCGTGC TGACTTCTGG 
AGATTCAGAG GTCTTCGCAG CTCCAAGAGA AATCTTCGAA AAAGCAGAGA GGATTTGTCC 
GCTCAGCCTG TTCAAACAAA GTTTCCAGCT TATGAAAGAG TGGTTCTTCG AGAAGCTGGA 
TTTCTGAGGC CTGTAACCAT TTTTGGACCA ATAGCTGATG TTGCCAGAGA AAAGCTGGCA 
AGAGAAGAAC CAGATATTTA TCAAATTGCA AAGAGTGAAC CACGAGACGC TGGAACTGAC 
CAACGTAGCT CTGGCATTAT TCGCCTGCAT ACAATAAAGC AAATCATAGA TCAAGACAAA 
CATGCTTTAT TAGATGTAAC ACCAAATGCA GTTGATCGTC TTAACTATGC CCAGTGGTAT 
CCAATTGTTG TATTTCTTAA CCCTGATTCT AAGCAAGGAG TAAAAACAAT GAGAATGAGG 
TTATGTCCAG AATCTCGGAA AAGTGCCAGG AAGTTATATG AGCGATCTCA TAAACTTCGT 
AAAAATAATC ACCATCTTTT TACAACTACA ATTAACTTAA ATTCAATGAA TGATGGTTGG 
TATGGTGCGC TGAAAGAAGC AATTCAACAA CAGCAAAACC AGCTGGTATG GGTTTCCGAG 
GGAAAGGCGG ATGGTGCTAC AAGTGATGAC CTTGATTTGC ATGATGATCG TCTGTCCTAC 
CTGTCAGCTC CAGGTAGTGA ATACTCAATG TATAGCACGG ACAGTAGACA CACTTCTGAC 
TATGAAGACA CAGACACAGA AGGCGGGGCC TACACTGATC AAGAACTAGA TGAAACTCTT 
AATGATGAGG TTGGGACTCC ACCGGAGTCT GCCATTACAC GGTCCTCTGA GCCTGTAAGA 
GAGGACTCCT CTGGAATGCA TCATGAAAAC CAAACATATC CTCCTTACTC ACCACAAGCG 
CAGCCACAAC CAATTCATAG AATAGACTCC CCTGGATTTA AGCCAGCCTC TCAACAGAAA 
GCAGAAGCTT CATCTCCAGT CCCTTACCTT TCGCCTGAAA CAAACCCAGC ATCATCAACC 
TCTGCTGTTA ATCATAATGT AAATTTAACT AATGTCAGAC TGGAGGAGCC CACCCCAGCT 
CCTTCCACCT CTTACTCACC ACAAGCTGAT TCTTTAAGAA CACCAAGTAC TGAGGCAGCT 
CACATAATGC TAAGAGATCA AGAACCATCA TTGTCGTCGC ATGTAGATCC AACAAAGGTG 
TATAGAAAGG ATCCATATCC CGAGGAAATG ATGAGGCAGA ACCATGTTTT GAAACAGCCA 
GCCGTTAGTC ACCCAGGGCA CAGGCCAGAC AAAGAGCCTA ATCTGACCTA TGAACCCCAA 
CTCCCATACG TAGAGAAACA AGCCAGCAGA GACCTCGAGC AGCCCACATA CAGATACGAG 
TCCTCAAGCT ATACGGACCA GTTTTCTCGA AACTATGAAC ATCGTCTGCG ATACGAAGAT 
CGCGTCCCCA TGTATGAAGA ACAGTGGTCA TATTATGATG ACAAACAGCC CTACCCATCT 
CGGCCACCTT TTGATAATCA GCACTCTCAA GACCTTGACT CCAGACAGCA TCCCGAAGAG 
TCCTCAGAAC GAGGGTACTT TCCACGTTTT GAAGAGCCAG CCCCTCTGTC TTACGACAGC 
AGACCACGTT ACGAACAGGC ACCTAGAGCA TCCGCCCTGC GGCACGAAGA GCAGCCAGCT 
CCTGGGTATG ACACACATGG TAGACTCAGA CCGGAAGCCC AGCCCCACCC TTCAGCAGGG 
CCCAAGCCCG CAGAGTCCAA GCAGTATTTT GAGCAATATT CACGCAGTTA CGAGCAAGTA 
CCACCCCAAG GATTTACCTC TAGAGCAGGT CATTTTGAGC CTCTCCATGG TGCTGCAGCT 
GTCCCTCCGC TGATACCTTC ATCTCAGCAT AAGCCAGAAG CTCTGCCTTC AAACACCAAA 
CCACTGCCTC CACCCCCAAC TCAAACCGAA GAAGAGGAAG ATCCAGCAAT GAAGCCACAG 
TCTGTACTCA CCAGAGTTAA GATGTTTGAA AACAAAAGAT CTGCATCCTT AGAGACCAAG 
AAGGATGTAA ATGACACTGG CAGTTTTAAG CCTCCAGAAG TAGCATCTAA ACCTTCAGGT 
GCTCCCATCA TTGGTCCCAA ACCCACTTCT CAGAATCAGT TCAGTGAACA TGACAAAACT 
CTGTACAGGA TCCCAGAACC TCAAAAACCT CAACTGAAGC CACCTGAAGA TATCGTTCGG 
TCCAATCATT ATGACCCTGA AGAAGATGAA GAATATTATC GAAAACAGCT GTCATACTTT 
GACCGAAGAA GTTTTGAGAA TAAGCCTCCT GCACACATTG CCGCCAGCCA TCTCTCCGAG 
CCTGCAAAGC CAGCTCATTC TCAGAATCAA TCAAATTTTT CTAGTTATTC TTCAAAGGGA 
AAGCCTCCTG AAGCTGATGG TGTGGATAGA TCATTTGGCG AGAAACGCTA TGAACCCATC 
CAGGCCACTC CCCCTCCTCC TCCATTGCCC TCGCAGTATG CCCAGCCATC TCAGCCTGTC 
ACCAGCGCGT CTCTCCACAT ACATTCTAAG GGAGCACATG GTGAAGGTAA TTCAGTGTCA 
TTGGATTTTC AGAATTCCTT AGTGTCCAAA CCAGACCCAC CTCCATCTCA GAATAAGCCA 
GCAACTTTCA GACCACCAAA CCGAGAAGAT ACTGCTCAGC CAGCTTTCTA TCCCCAGAAA 
AGTTTTCCAG ATAAAGCCCC AGTTAATGGA ACTGAACAGA CTCAGAAAAC AGTCACTCCA 
GCATACAATC GATTCACACC AAAACCATAT ACAAGTTCTG CCCGACCATT TGAACGCAAG 
TTTGAAAGTC CTAAATTCAA TCACAATCTT CTGCCAAGTG AAACTGCACA TAAACCTGAA 
TTGTCTTCAA AAACTCCCAC TTCTCCAAAA ACTCTTGTGA AATCGCACAG TTTGGCACAG 
CCTCCTGAGT TTGACAGTGG AGTTGAAACT TTCTCTATCC ATGCAGAGAA GCCTAAATAT 
CAAATAAATA ATATCAGCAC AGTGCCTAAA GCTATTCCTG TGAGTCCTTC AGCTGTGGAA 
GAGGATGAAG ATGAAGATGG TCATACTGTG GTGGCCACAG CCCGAGGCAT ATTTAACAGC 
AATGGGGGCG TGCTGAGTTC CATAGAAACT GGCGTTAGTA TAATTATCCC TCAAGGAGCC 
ATTCCCGAAG GAGTTGAGCA GGAAATCTAT TTCAAGGTCT GCCGGGACAA CAGCATCCTT 
CCACCTTTAG ATAAAGAGAA AGGTGAAACA CTGCTGAGTC CTTTGGTGAT GTGTGGTCCC 
CATGGCCTCA AGTTCCTGAA GCCTGTGGAG CTGCGCTTAC CACACTGTGC GTCCATGACT 
CCTGACGGTT GGTCTTTTGC TCTAAAATCA TCCGACTCCT CGTCGGGTGA TCCTAAAACC 
TGGCAAAACA AGTGTCTTCC CGGAGATCCA AATTATCTCG TTGGAGCAAA CTGTGTTTCT 
GTCCTTATTG ACCACTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_016927842.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016783331.1 Pan troglodytes tight junction protein 1 (TJP1), transcript variant X12, mRNA.

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CloneID	OPc69385
Clone ID Related Accession (Same CDS sequence)	XM_016927842.1
Accession Version	XM_016927842.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5079bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	tight junction protein ZO-1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006482.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGAA ATACGTTTTC AATGGATTGC CACAGTAAGA GCACAGCAAT GGAGGAAACA 
GCTATATGGG AACAACATAC AGTGACGCTT CACAGGGCTC CTGGATTTGG GTTTGGAATT 
GCAATATCTG GTGGACGAGA TAATCCTCAT TTTCAGAGTG GGGAAACGTC AATAGTGATT 
TCAGATGTGC TGAAAGGAGG ACCAGCTGAA GGACAGCTAC AGGAAAATGA CCGAGTTGCA 
ATGGTTAACG GAGTTTCAAT GGATAATGTT GAACATGCTT TTGCTGTTCA GCAACTAAGG 
AAAAGTGGGA AAAATGCAAA AATTACAATT AGAAGGAAGA AGAAAGTTCA AATACCAGTA 
AGTCGTCCTG ATCCTGAACC AGTATCTGAT AATGAAGAAG ATAGTTATGA TGAGGAAATA 
CATGATCCAA GAAGTGGCCG GAGTGGTGTG GTTAACAGAA GGAGTGAGAA GATTTGGCCG 
AGGGATAGAA GTGCAAGTAG AGAGAGGAGC TTGTCCCCGC GGTCAGACAG GCGGTCAGTG 
GCTTCCAGCC AGCCCGCTAA ACCTACTAAA GTCACACTGG TGAAATCCCG GAAAAATGAA 
GAATATGGTC TTCGATTGGC AAGCCATATA TTTGTTAAGG AAATTTCACA AGATAGTTTG 
GCAGCAAGAG ATGGCAATAT TCAAGAAGGT GATGTTGTAT TGAAGATAAA TGGTACTGTG 
ACAGAAAATA TGTCATTGAC AGATGCAAAG ACATTGATAG AAAGGTCTAA AGGCAAATTA 
AAAATGGTAG TTCAAAGAGA TGAACGGGCT ACGCTATTGA ATGTCCCTGA TCTTTCTGAC 
AGCATCCACT CTGCTAATGC CTCTGAGAGA GACGACATTT CAGAAATTCA GTCACTGGCA 
TCAGATCATT CTGGTCGATC ACACGATAGG CCTCCCCGCC GCAGCCGGTC ACGATCTCCT 
GACCAGCGGT CAGAGCCTTC TGATCATTCC AGGCACTCGC CACAGCAGCC AAGCAATGGC 
AGTCTCCGGA GTAGAGATGA AGAGAGAATT TCTAAACCTG GGGCTGTCTC AACTCCTGTA 
AAGCATGCTG ATGATCACAC ACCTAAAACA GTGGAAGAAG TTACAGTTGA AAGAAATGAG 
AAACAAACAC CTTCTCTTCC AGAACCAAAG CCTGTGTATG CCCAAGTTGG GCAGCCAGAT 
GTGGATTTAC CTGTCAGTCC ATCTGATGGT GTCCTACCTA ATTCAACTCA TGAAGATGGG 
ATTCTTCGGC CCAGCATGAA ATTGGTAAAA TTCAGAAAAG GAGATAGTGT GGGTTTGCGG 
CTGGCTGGTG GAAATGATGT TGGAATATTT GTAGCTGGCG TTCTAGAAGA TAGCCCTGCA 
GCCAAGGAAG GCTTAGAGGA AGGTGATCAA ATTCTCAGGG TAAACAACGT AGATTTTACA 
AATATCATAA GAGAAGAAGC CGTCCTTTTC CTGCTTGACC TCCCTAAAGG AGAAGAAGTG 
ACCATATTGG CTCAGAAGAA GAAGGATGTT TATCGTCGCA TTGTAGAATC AGATGTAGGA 
GATTCTTTCT ATATTAGAAC CCATTTTGAA TATGAAAAGG AATCTCCCTA TGGACTTAGT 
TTTAACAAAG GAGAGGTGTT CCGTGTTGTG GATACCTTGT ACAATGGAAA ACTGGGCTCT 
TGGCTTGCTA TTCGAATTGG TAAAAATCAT AAGGAGGTAG AACGAGGCAT CATCCCTAAT 
AAGAACAGAG CTGAGCAGCT AGCCAGTGTA CAGTATACAC TTCCAAAAAC AGCAGGCGGA 
GACCGTGCTG ACTTCTGGAG ATTCAGAGGT CTTCGCAGCT CCAAGAGAAA TCTTCGAAAA 
AGCAGAGAGG ATTTGTCCGC TCAGCCTGTT CAAACAAAGT TTCCAGCTTA TGAAAGAGTG 
GTTCTTCGAG AAGCTGGATT TCTGAGGCCT GTAACCATTT TTGGACCAAT AGCTGATGTT 
GCCAGAGAAA AGCTGGCAAG AGAAGAACCA GATATTTATC AAATTGCAAA GAGTGAACCA 
CGAGACGCTG GAACTGACCA ACGTAGCTCT GGCATTATTC GCCTGCATAC AATAAAGCAA 
ATCATAGATC AAGACAAACA TGCTTTATTA GATGTAACAC CAAATGCAGT TGATCGTCTT 
AACTATGCCC AGTGGTATCC AATTGTTGTA TTTCTTAACC CTGATTCTAA GCAAGGAGTA 
AAAACAATGA GAATGAGGTT ATGTCCAGAA TCTCGGAAAA GTGCCAGGAA GTTATATGAG 
CGATCTCATA AACTTCGTAA AAATAATCAC CATCTTTTTA CAACTACAAT TAACTTAAAT 
TCAATGAATG ATGGTTGGTA TGGTGCGCTG AAAGAAGCAA TTCAACAACA GCAAAACCAG 
CTGGTATGGG TTTCCGAGGG AAAGGCGGAT GGTGCTACAA GTGATGACCT TGATTTGCAT 
GATGATCGTC TGTCCTACCT GTCAGCTCCA GGTAGTGAAT ACTCAATGTA TAGCACGGAC 
AGTAGACACA CTTCTGACTA TGAAGACACA GACACAGAAG GCGGGGCCTA CACTGATCAA 
GAACTAGATG AAACTCTTAA TGATGAGGTT GGGACTCCAC CGGAGTCTGC CATTACACGG 
TCCTCTGAGC CTGTAAGAGA GGACTCCTCT GGAATGCATC ATGAAAACCA AACATATCCT 
CCTTACTCAC CACAAGCGCA GCCACAACCA ATTCATAGAA TAGACTCCCC TGGATTTAAG 
CCAGCCTCTC AACAGGTGTA TAGAAAGGAT CCATATCCCG AGGAAATGAT GAGGCAGAAC 
CATGTTTTGA AACAGCCAGC CGTTAGTCAC CCAGGGCACA GGCCAGACAA AGAGCCTAAT 
CTGACCTATG AACCCCAACT CCCATACGTA GAGAAACAAG CCAGCAGAGA CCTCGAGCAG 
CCCACATACA GATACGAGTC CTCAAGCTAT ACGGACCAGT TTTCTCGAAA CTATGAACAT 
CGTCTGCGAT ACGAAGATCG CGTCCCCATG TATGAAGAAC AGTGGTCATA TTATGATGAC 
AAACAGCCCT ACCCATCTCG GCCACCTTTT GATAATCAGC ACTCTCAAGA CCTTGACTCC 
AGACAGCATC CCGAAGAGTC CTCAGAACGA GGGTACTTTC CACGTTTTGA AGAGCCAGCC 
CCTCTGTCTT ACGACAGCAG ACCACGTTAC GAACAGGCAC CTAGAGCATC CGCCCTGCGG 
CACGAAGAGC AGCCAGCTCC TGGGTATGAC ACACATGGTA GACTCAGACC GGAAGCCCAG 
CCCCACCCTT CAGCAGGGCC CAAGCCCGCA GAGTCCAAGC AGTATTTTGA GCAATATTCA 
CGCAGTTACG AGCAAGTACC ACCCCAAGGA TTTACCTCTA GAGCAGGTCA TTTTGAGCCT 
CTCCATGGTG CTGCAGCTGT CCCTCCGCTG ATACCTTCAT CTCAGCATAA GCCAGAAGCT 
CTGCCTTCAA ACACCAAACC ACTGCCTCCA CCCCCAACTC AAACCGAAGA AGAGGAAGAT 
CCAGCAATGA AGCCACAGTC TGTACTCACC AGAGTTAAGA TGTTTGAAAA CAAAAGATCT 
GCATCCTTAG AGACCAAGAA GGATGTAAAT GACACTGGCA GTTTTAAGCC TCCAGAAGTA 
GCATCTAAAC CTTCAGGTGC TCCCATCATT GGTCCCAAAC CCACTTCTCA GAATCAGTTC 
AGTGAACATG ACAAAACTCT GTACAGGATC CCAGAACCTC AAAAACCTCA ACTGAAGCCA 
CCTGAAGATA TCGTTCGGTC CAATCATTAT GACCCTGAAG AAGATGAAGA ATATTATCGA 
AAACAGCTGT CATACTTTGA CCGAAGAAGT TTTGAGAATA AGCCTCCTGC ACACATTGCC 
GCCAGCCATC TCTCCGAGCC TGCAAAGCCA GCTCATTCTC AGAATCAATC AAATTTTTCT 
AGTTATTCTT CAAAGGGAAA GCCTCCTGAA GCTGATGGTG TGGATAGATC ATTTGGCGAG 
AAACGCTATG AACCCATCCA GGCCACTCCC CCTCCTCCTC CATTGCCCTC GCAGTATGCC 
CAGCCATCTC AGCCTGTCAC CAGCGCGTCT CTCCACATAC ATTCTAAGGG AGCACATGGT 
GAAGGTAATT CAGTGTCATT GGATTTTCAG AATTCCTTAG TGTCCAAACC AGACCCACCT 
CCATCTCAGA ATAAGCCAGC AACTTTCAGA CCACCAAACC GAGAAGATAC TGCTCAGCCA 
GCTTTCTATC CCCAGAAAAG TTTTCCAGAT AAAGCCCCAG TTAATGGAAC TGAACAGACT 
CAGAAAACAG TCACTCCAGC ATACAATCGA TTCACACCAA AACCATATAC AAGTTCTGCC 
CGACCATTTG AACGCAAGTT TGAAAGTCCT AAATTCAATC ACAATCTTCT GCCAAGTGAA 
ACTGCACATA AACCTGAATT GTCTTCAAAA ACTCCCACTT CTCCAAAAAC TCTTGTGAAA 
TCGCACAGTT TGGCACAGCC TCCTGAGTTT GACAGTGGAG TTGAAACTTT CTCTATCCAT 
GCAGAGAAGC CTAAATATCA AATAAATAAT ATCAGCACAG TGCCTAAAGC TATTCCTGTG 
AGTCCTTCAG CTGTGGAAGA GGATGAAGAT GAAGATGGTC ATACTGTGGT GGCCACAGCC 
CGAGGCATAT TTAACAGCAA TGGGGGCGTG CTGAGTTCCA TAGAAACTGG CGTTAGTATA 
ATTATCCCTC AAGGAGCCAT TCCCGAAGGA GTTGAGCAGG AAATCTATTT CAAGGTCTGC 
CGGGACAACA GCATCCTTCC ACCTTTAGAT AAAGAGAAAG GTGAAACACT GCTGAGTCCT 
TTGGTGATGT GTGGTCCCCA TGGCCTCAAG TTCCTGAAGC CTGTGGAGCT GCGCTTACCA 
CACTGTGCGT CCATGACTCC TGACGGTTGG TCTTTTGCTC TAAAATCATC CGACTCCTCG 
TCGGGTGATC CTAAAACCTG GCAAAACAAG TGTCTTCCCG GAGATCCAAA TTATCTCGTT 
GGAGCAAACT GTGTTTCTGT CCTTATTGAC CACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016783331.1
CDS	110..5188
Translation	MERNTFSMDCHSKSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQPAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPELSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_016927842.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes tight junction protein 1 (TJP1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016927842.1

ORF Insert Sequence:

ATGGAGAGAA ATACGTTTTC AATGGATTGC CACAGTAAGA GCACAGCAAT GGAGGAAACA 
GCTATATGGG AACAACATAC AGTGACGCTT CACAGGGCTC CTGGATTTGG GTTTGGAATT 
GCAATATCTG GTGGACGAGA TAATCCTCAT TTTCAGAGTG GGGAAACGTC AATAGTGATT 
TCAGATGTGC TGAAAGGAGG ACCAGCTGAA GGACAGCTAC AGGAAAATGA CCGAGTTGCA 
ATGGTTAACG GAGTTTCAAT GGATAATGTT GAACATGCTT TTGCTGTTCA GCAACTAAGG 
AAAAGTGGGA AAAATGCAAA AATTACAATT AGAAGGAAGA AGAAAGTTCA AATACCAGTA 
AGTCGTCCTG ATCCTGAACC AGTATCTGAT AATGAAGAAG ATAGTTATGA TGAGGAAATA 
CATGATCCAA GAAGTGGCCG GAGTGGTGTG GTTAACAGAA GGAGTGAGAA GATTTGGCCG 
AGGGATAGAA GTGCAAGTAG AGAGAGGAGC TTGTCCCCGC GGTCAGACAG GCGGTCAGTG 
GCTTCCAGCC AGCCCGCTAA ACCTACTAAA GTCACACTGG TGAAATCCCG GAAAAATGAA 
GAATATGGTC TTCGATTGGC AAGCCATATA TTTGTTAAGG AAATTTCACA AGATAGTTTG 
GCAGCAAGAG ATGGCAATAT TCAAGAAGGT GATGTTGTAT TGAAGATAAA TGGTACTGTG 
ACAGAAAATA TGTCATTGAC AGATGCAAAG ACATTGATAG AAAGGTCTAA AGGCAAATTA 
AAAATGGTAG TTCAAAGAGA TGAACGGGCT ACGCTATTGA ATGTCCCTGA TCTTTCTGAC 
AGCATCCACT CTGCTAATGC CTCTGAGAGA GACGACATTT CAGAAATTCA GTCACTGGCA 
TCAGATCATT CTGGTCGATC ACACGATAGG CCTCCCCGCC GCAGCCGGTC ACGATCTCCT 
GACCAGCGGT CAGAGCCTTC TGATCATTCC AGGCACTCGC CACAGCAGCC AAGCAATGGC 
AGTCTCCGGA GTAGAGATGA AGAGAGAATT TCTAAACCTG GGGCTGTCTC AACTCCTGTA 
AAGCATGCTG ATGATCACAC ACCTAAAACA GTGGAAGAAG TTACAGTTGA AAGAAATGAG 
AAACAAACAC CTTCTCTTCC AGAACCAAAG CCTGTGTATG CCCAAGTTGG GCAGCCAGAT 
GTGGATTTAC CTGTCAGTCC ATCTGATGGT GTCCTACCTA ATTCAACTCA TGAAGATGGG 
ATTCTTCGGC CCAGCATGAA ATTGGTAAAA TTCAGAAAAG GAGATAGTGT GGGTTTGCGG 
CTGGCTGGTG GAAATGATGT TGGAATATTT GTAGCTGGCG TTCTAGAAGA TAGCCCTGCA 
GCCAAGGAAG GCTTAGAGGA AGGTGATCAA ATTCTCAGGG TAAACAACGT AGATTTTACA 
AATATCATAA GAGAAGAAGC CGTCCTTTTC CTGCTTGACC TCCCTAAAGG AGAAGAAGTG 
ACCATATTGG CTCAGAAGAA GAAGGATGTT TATCGTCGCA TTGTAGAATC AGATGTAGGA 
GATTCTTTCT ATATTAGAAC CCATTTTGAA TATGAAAAGG AATCTCCCTA TGGACTTAGT 
TTTAACAAAG GAGAGGTGTT CCGTGTTGTG GATACCTTGT ACAATGGAAA ACTGGGCTCT 
TGGCTTGCTA TTCGAATTGG TAAAAATCAT AAGGAGGTAG AACGAGGCAT CATCCCTAAT 
AAGAACAGAG CTGAGCAGCT AGCCAGTGTA CAGTATACAC TTCCAAAAAC AGCAGGCGGA 
GACCGTGCTG ACTTCTGGAG ATTCAGAGGT CTTCGCAGCT CCAAGAGAAA TCTTCGAAAA 
AGCAGAGAGG ATTTGTCCGC TCAGCCTGTT CAAACAAAGT TTCCAGCTTA TGAAAGAGTG 
GTTCTTCGAG AAGCTGGATT TCTGAGGCCT GTAACCATTT TTGGACCAAT AGCTGATGTT 
GCCAGAGAAA AGCTGGCAAG AGAAGAACCA GATATTTATC AAATTGCAAA GAGTGAACCA 
CGAGACGCTG GAACTGACCA ACGTAGCTCT GGCATTATTC GCCTGCATAC AATAAAGCAA 
ATCATAGATC AAGACAAACA TGCTTTATTA GATGTAACAC CAAATGCAGT TGATCGTCTT 
AACTATGCCC AGTGGTATCC AATTGTTGTA TTTCTTAACC CTGATTCTAA GCAAGGAGTA 
AAAACAATGA GAATGAGGTT ATGTCCAGAA TCTCGGAAAA GTGCCAGGAA GTTATATGAG 
CGATCTCATA AACTTCGTAA AAATAATCAC CATCTTTTTA CAACTACAAT TAACTTAAAT 
TCAATGAATG ATGGTTGGTA TGGTGCGCTG AAAGAAGCAA TTCAACAACA GCAAAACCAG 
CTGGTATGGG TTTCCGAGGG AAAGGCGGAT GGTGCTACAA GTGATGACCT TGATTTGCAT 
GATGATCGTC TGTCCTACCT GTCAGCTCCA GGTAGTGAAT ACTCAATGTA TAGCACGGAC 
AGTAGACACA CTTCTGACTA TGAAGACACA GACACAGAAG GCGGGGCCTA CACTGATCAA 
GAACTAGATG AAACTCTTAA TGATGAGGTT GGGACTCCAC CGGAGTCTGC CATTACACGG 
TCCTCTGAGC CTGTAAGAGA GGACTCCTCT GGAATGCATC ATGAAAACCA AACATATCCT 
CCTTACTCAC CACAAGCGCA GCCACAACCA ATTCATAGAA TAGACTCCCC TGGATTTAAG 
CCAGCCTCTC AACAGGTGTA TAGAAAGGAT CCATATCCCG AGGAAATGAT GAGGCAGAAC 
CATGTTTTGA AACAGCCAGC CGTTAGTCAC CCAGGGCACA GGCCAGACAA AGAGCCTAAT 
CTGACCTATG AACCCCAACT CCCATACGTA GAGAAACAAG CCAGCAGAGA CCTCGAGCAG 
CCCACATACA GATACGAGTC CTCAAGCTAT ACGGACCAGT TTTCTCGAAA CTATGAACAT 
CGTCTGCGAT ACGAAGATCG CGTCCCCATG TATGAAGAAC AGTGGTCATA TTATGATGAC 
AAACAGCCCT ACCCATCTCG GCCACCTTTT GATAATCAGC ACTCTCAAGA CCTTGACTCC 
AGACAGCATC CCGAAGAGTC CTCAGAACGA GGGTACTTTC CACGTTTTGA AGAGCCAGCC 
CCTCTGTCTT ACGACAGCAG ACCACGTTAC GAACAGGCAC CTAGAGCATC CGCCCTGCGG 
CACGAAGAGC AGCCAGCTCC TGGGTATGAC ACACATGGTA GACTCAGACC GGAAGCCCAG 
CCCCACCCTT CAGCAGGGCC CAAGCCCGCA GAGTCCAAGC AGTATTTTGA GCAATATTCA 
CGCAGTTACG AGCAAGTACC ACCCCAAGGA TTTACCTCTA GAGCAGGTCA TTTTGAGCCT 
CTCCATGGTG CTGCAGCTGT CCCTCCGCTG ATACCTTCAT CTCAGCATAA GCCAGAAGCT 
CTGCCTTCAA ACACCAAACC ACTGCCTCCA CCCCCAACTC AAACCGAAGA AGAGGAAGAT 
CCAGCAATGA AGCCACAGTC TGTACTCACC AGAGTTAAGA TGTTTGAAAA CAAAAGATCT 
GCATCCTTAG AGACCAAGAA GGATGTAAAT GACACTGGCA GTTTTAAGCC TCCAGAAGTA 
GCATCTAAAC CTTCAGGTGC TCCCATCATT GGTCCCAAAC CCACTTCTCA GAATCAGTTC 
AGTGAACATG ACAAAACTCT GTACAGGATC CCAGAACCTC AAAAACCTCA ACTGAAGCCA 
CCTGAAGATA TCGTTCGGTC CAATCATTAT GACCCTGAAG AAGATGAAGA ATATTATCGA 
AAACAGCTGT CATACTTTGA CCGAAGAAGT TTTGAGAATA AGCCTCCTGC ACACATTGCC 
GCCAGCCATC TCTCCGAGCC TGCAAAGCCA GCTCATTCTC AGAATCAATC AAATTTTTCT 
AGTTATTCTT CAAAGGGAAA GCCTCCTGAA GCTGATGGTG TGGATAGATC ATTTGGCGAG 
AAACGCTATG AACCCATCCA GGCCACTCCC CCTCCTCCTC CATTGCCCTC GCAGTATGCC 
CAGCCATCTC AGCCTGTCAC CAGCGCGTCT CTCCACATAC ATTCTAAGGG AGCACATGGT 
GAAGGTAATT CAGTGTCATT GGATTTTCAG AATTCCTTAG TGTCCAAACC AGACCCACCT 
CCATCTCAGA ATAAGCCAGC AACTTTCAGA CCACCAAACC GAGAAGATAC TGCTCAGCCA 
GCTTTCTATC CCCAGAAAAG TTTTCCAGAT AAAGCCCCAG TTAATGGAAC TGAACAGACT 
CAGAAAACAG TCACTCCAGC ATACAATCGA TTCACACCAA AACCATATAC AAGTTCTGCC 
CGACCATTTG AACGCAAGTT TGAAAGTCCT AAATTCAATC ACAATCTTCT GCCAAGTGAA 
ACTGCACATA AACCTGAATT GTCTTCAAAA ACTCCCACTT CTCCAAAAAC TCTTGTGAAA 
TCGCACAGTT TGGCACAGCC TCCTGAGTTT GACAGTGGAG TTGAAACTTT CTCTATCCAT 
GCAGAGAAGC CTAAATATCA AATAAATAAT ATCAGCACAG TGCCTAAAGC TATTCCTGTG 
AGTCCTTCAG CTGTGGAAGA GGATGAAGAT GAAGATGGTC ATACTGTGGT GGCCACAGCC 
CGAGGCATAT TTAACAGCAA TGGGGGCGTG CTGAGTTCCA TAGAAACTGG CGTTAGTATA 
ATTATCCCTC AAGGAGCCAT TCCCGAAGGA GTTGAGCAGG AAATCTATTT CAAGGTCTGC 
CGGGACAACA GCATCCTTCC ACCTTTAGAT AAAGAGAAAG GTGAAACACT GCTGAGTCCT 
TTGGTGATGT GTGGTCCCCA TGGCCTCAAG TTCCTGAAGC CTGTGGAGCT GCGCTTACCA 
CACTGTGCGT CCATGACTCC TGACGGTTGG TCTTTTGCTC TAAAATCATC CGACTCCTCG 
TCGGGTGATC CTAAAACCTG GCAAAACAAG TGTCTTCCCG GAGATCCAAA TTATCTCGTT 
GGAGCAAACT GTGTTTCTGT CCTTATTGAC CACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_001163045.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_001163045.2 Pan troglodytes tight junction protein 1 (TJP1), transcript variant X13, mRNA.

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CloneID	OPc69386
Clone ID Related Accession (Same CDS sequence)	XM_001163045.3
Accession Version	XM_001163045.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	tight junction protein ZO-1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006482.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_001163045.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGGT TTGGAATTGC AATATCTGGT 
GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG 
AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA 
GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT 
CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA 
AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT 
GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG 
CCCGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT 
CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT 
GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT 
GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA 
GAGCCTTCTG ATCATTCCAG GCACTCGCCA CAGCAGCCAA GCAATGGCAG TCTCCGGAGT 
AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT 
GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT 
TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AGCCAGATGT GGATTTACCT 
GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC 
AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA 
AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA 
GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT 
CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC 
TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT 
TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA 
GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA 
GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG 
TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA 
ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATATGAGCG ATCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT 
TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA 
CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA 
CAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA 
CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA 
CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA 
TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC 
GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC 
GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC 
GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG 
CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA 
GCAGGGCCCA AGCCCGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG 
CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT 
GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC 
ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG 
CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG 
ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT 
TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAGTTCAG TGAACATGAC 
AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATC 
GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA 
TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC 
TCCGAGCCTG CAAAGCCAGC TCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA 
AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA 
CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG 
CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA 
GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT 
AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGCCAGC TTTCTATCCC 
CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC 
ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA 
CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA 
CCTGAATTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG 
GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT 
AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT 
GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT 
AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGCG TTAGTATAAT TATCCCTCAA 
GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC 
ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT 
GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGATCCT 
AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT 
GTTTCTGTCC TTATTGACCA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001163045.2
CDS	476..5482
Translation	MSARAAAAKSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQPAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPELSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_001163045.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes tight junction protein 1 (TJP1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001163045.3

ORF Insert Sequence:

ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGGT TTGGAATTGC AATATCTGGT 
GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG 
AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA 
GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT 
CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA 
AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT 
GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG 
CCCGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT 
CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT 
GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT 
GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA 
GAGCCTTCTG ATCATTCCAG GCACTCGCCA CAGCAGCCAA GCAATGGCAG TCTCCGGAGT 
AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT 
GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT 
TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AGCCAGATGT GGATTTACCT 
GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC 
AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA 
AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA 
GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT 
CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC 
TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT 
TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA 
GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA 
GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG 
TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA 
ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATATGAGCG ATCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT 
TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA 
CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA 
CAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA 
CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA 
CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA 
TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC 
GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC 
GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC 
GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG 
CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA 
GCAGGGCCCA AGCCCGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG 
CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT 
GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC 
ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG 
CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG 
ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT 
TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAGTTCAG TGAACATGAC 
AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATC 
GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA 
TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC 
TCCGAGCCTG CAAAGCCAGC TCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA 
AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA 
CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG 
CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA 
GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT 
AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGCCAGC TTTCTATCCC 
CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC 
ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA 
CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA 
CCTGAATTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG 
GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT 
AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT 
GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT 
AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGCG TTAGTATAAT TATCCCTCAA 
GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC 
ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT 
GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGATCCT 
AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT 
GTTTCTGTCC TTATTGACCA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_016927843.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016783332.1 Pan troglodytes tight junction protein 1 (TJP1), transcript variant X14, mRNA.

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CloneID	OPc69387
Clone ID Related Accession (Same CDS sequence)	XM_016927843.1
Accession Version	XM_016927843.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4620bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	tight junction protein ZO-1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006482.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATAA ATGGTACTGT GACAGAAAAT ATGTCATTGA CAGATGCAAA GACATTGATA 
GAAAGGTCTA AAGGCAAATT AAAAATGGTA GTTCAAAGAG ATGAACGGGC TACGCTATTG 
AATGTCCCTG ATCTTTCTGA CAGCATCCAC TCTGCTAATG CCTCTGAGAG AGACGACATT 
TCAGAAATTC AGTCACTGGC ATCAGATCAT TCTGGTCGAT CACACGATAG GCCTCCCCGC 
CGCAGCCGGT CACGATCTCC TGACCAGCGG TCAGAGCCTT CTGATCATTC CAGGCACTCG 
CCACAGCAGC CAAGCAATGG CAGTCTCCGG AGTAGAGATG AAGAGAGAAT TTCTAAACCT 
GGGGCTGTCT CAACTCCTGT AAAGCATGCT GATGATCACA CACCTAAAAC AGTGGAAGAA 
GTTACAGTTG AAAGAAATGA GAAACAAACA CCTTCTCTTC CAGAACCAAA GCCTGTGTAT 
GCCCAAGTTG GGCAGCCAGA TGTGGATTTA CCTGTCAGTC CATCTGATGG TGTCCTACCT 
AATTCAACTC ATGAAGATGG GATTCTTCGG CCCAGCATGA AATTGGTAAA ATTCAGAAAA 
GGAGATAGTG TGGGTTTGCG GCTGGCTGGT GGAAATGATG TTGGAATATT TGTAGCTGGC 
GTTCTAGAAG ATAGCCCTGC AGCCAAGGAA GGCTTAGAGG AAGGTGATCA AATTCTCAGG 
GTAAACAACG TAGATTTTAC AAATATCATA AGAGAAGAAG CCGTCCTTTT CCTGCTTGAC 
CTCCCTAAAG GAGAAGAAGT GACCATATTG GCTCAGAAGA AGAAGGATGT TTATCGTCGC 
ATTGTAGAAT CAGATGTAGG AGATTCTTTC TATATTAGAA CCCATTTTGA ATATGAAAAG 
GAATCTCCCT ATGGACTTAG TTTTAACAAA GGAGAGGTGT TCCGTGTTGT GGATACCTTG 
TACAATGGAA AACTGGGCTC TTGGCTTGCT ATTCGAATTG GTAAAAATCA TAAGGAGGTA 
GAACGAGGCA TCATCCCTAA TAAGAACAGA GCTGAGCAGC TAGCCAGTGT ACAGTATACA 
CTTCCAAAAA CAGCAGGCGG AGACCGTGCT GACTTCTGGA GATTCAGAGG TCTTCGCAGC 
TCCAAGAGAA ATCTTCGAAA AAGCAGAGAG GATTTGTCCG CTCAGCCTGT TCAAACAAAG 
TTTCCAGCTT ATGAAAGAGT GGTTCTTCGA GAAGCTGGAT TTCTGAGGCC TGTAACCATT 
TTTGGACCAA TAGCTGATGT TGCCAGAGAA AAGCTGGCAA GAGAAGAACC AGATATTTAT 
CAAATTGCAA AGAGTGAACC ACGAGACGCT GGAACTGACC AACGTAGCTC TGGCATTATT 
CGCCTGCATA CAATAAAGCA AATCATAGAT CAAGACAAAC ATGCTTTATT AGATGTAACA 
CCAAATGCAG TTGATCGTCT TAACTATGCC CAGTGGTATC CAATTGTTGT ATTTCTTAAC 
CCTGATTCTA AGCAAGGAGT AAAAACAATG AGAATGAGGT TATGTCCAGA ATCTCGGAAA 
AGTGCCAGGA AGTTATATGA GCGATCTCAT AAACTTCGTA AAAATAATCA CCATCTTTTT 
ACAACTACAA TTAACTTAAA TTCAATGAAT GATGGTTGGT ATGGTGCGCT GAAAGAAGCA 
ATTCAACAAC AGCAAAACCA GCTGGTATGG GTTTCCGAGG GAAAGGCGGA TGGTGCTACA 
AGTGATGACC TTGATTTGCA TGATGATCGT CTGTCCTACC TGTCAGCTCC AGGTAGTGAA 
TACTCAATGT ATAGCACGGA CAGTAGACAC ACTTCTGACT ATGAAGACAC AGACACAGAA 
GGCGGGGCCT ACACTGATCA AGAACTAGAT GAAACTCTTA ATGATGAGGT TGGGACTCCA 
CCGGAGTCTG CCATTACACG GTCCTCTGAG CCTGTAAGAG AGGACTCCTC TGGAATGCAT 
CATGAAAACC AAACATATCC TCCTTACTCA CCACAAGCGC AGCCACAACC AATTCATAGA 
ATAGACTCCC CTGGATTTAA GCCAGCCTCT CAACAGAAAG CAGAAGCTTC ATCTCCAGTC 
CCTTACCTTT CGCCTGAAAC AAACCCAGCA TCATCAACCT CTGCTGTTAA TCATAATGTA 
AATTTAACTA ATGTCAGACT GGAGGAGCCC ACCCCAGCTC CTTCCACCTC TTACTCACCA 
CAAGCTGATT CTTTAAGAAC ACCAAGTACT GAGGCAGCTC ACATAATGCT AAGAGATCAA 
GAACCATCAT TGTCGTCGCA TGTAGATCCA ACAAAGGTGT ATAGAAAGGA TCCATATCCC 
GAGGAAATGA TGAGGCAGAA CCATGTTTTG AAACAGCCAG CCGTTAGTCA CCCAGGGCAC 
AGGCCAGACA AAGAGCCTAA TCTGACCTAT GAACCCCAAC TCCCATACGT AGAGAAACAA 
GCCAGCAGAG ACCTCGAGCA GCCCACATAC AGATACGAGT CCTCAAGCTA TACGGACCAG 
TTTTCTCGAA ACTATGAACA TCGTCTGCGA TACGAAGATC GCGTCCCCAT GTATGAAGAA 
CAGTGGTCAT ATTATGATGA CAAACAGCCC TACCCATCTC GGCCACCTTT TGATAATCAG 
CACTCTCAAG ACCTTGACTC CAGACAGCAT CCCGAAGAGT CCTCAGAACG AGGGTACTTT 
CCACGTTTTG AAGAGCCAGC CCCTCTGTCT TACGACAGCA GACCACGTTA CGAACAGGCA 
CCTAGAGCAT CCGCCCTGCG GCACGAAGAG CAGCCAGCTC CTGGGTATGA CACACATGGT 
AGACTCAGAC CGGAAGCCCA GCCCCACCCT TCAGCAGGGC CCAAGCCCGC AGAGTCCAAG 
CAGTATTTTG AGCAATATTC ACGCAGTTAC GAGCAAGTAC CACCCCAAGG ATTTACCTCT 
AGAGCAGGTC ATTTTGAGCC TCTCCATGGT GCTGCAGCTG TCCCTCCGCT GATACCTTCA 
TCTCAGCATA AGCCAGAAGC TCTGCCTTCA AACACCAAAC CACTGCCTCC ACCCCCAACT 
CAAACCGAAG AAGAGGAAGA TCCAGCAATG AAGCCACAGT CTGTACTCAC CAGAGTTAAG 
ATGTTTGAAA ACAAAAGATC TGCATCCTTA GAGACCAAGA AGGATGTAAA TGACACTGGC 
AGTTTTAAGC CTCCAGAAGT AGCATCTAAA CCTTCAGGTG CTCCCATCAT TGGTCCCAAA 
CCCACTTCTC AGAATCAGTT CAGTGAACAT GACAAAACTC TGTACAGGAT CCCAGAACCT 
CAAAAACCTC AACTGAAGCC ACCTGAAGAT ATCGTTCGGT CCAATCATTA TGACCCTGAA 
GAAGATGAAG AATATTATCG AAAACAGCTG TCATACTTTG ACCGAAGAAG TTTTGAGAAT 
AAGCCTCCTG CACACATTGC CGCCAGCCAT CTCTCCGAGC CTGCAAAGCC AGCTCATTCT 
CAGAATCAAT CAAATTTTTC TAGTTATTCT TCAAAGGGAA AGCCTCCTGA AGCTGATGGT 
GTGGATAGAT CATTTGGCGA GAAACGCTAT GAACCCATCC AGGCCACTCC CCCTCCTCCT 
CCATTGCCCT CGCAGTATGC CCAGCCATCT CAGCCTGTCA CCAGCGCGTC TCTCCACATA 
CATTCTAAGG GAGCACATGG TGAAGGTAAT TCAGTGTCAT TGGATTTTCA GAATTCCTTA 
GTGTCCAAAC CAGACCCACC TCCATCTCAG AATAAGCCAG CAACTTTCAG ACCACCAAAC 
CGAGAAGATA CTGCTCAGCC AGCTTTCTAT CCCCAGAAAA GTTTTCCAGA TAAAGCCCCA 
GTTAATGGAA CTGAACAGAC TCAGAAAACA GTCACTCCAG CATACAATCG ATTCACACCA 
AAACCATATA CAAGTTCTGC CCGACCATTT GAACGCAAGT TTGAAAGTCC TAAATTCAAT 
CACAATCTTC TGCCAAGTGA AACTGCACAT AAACCTGAAT TGTCTTCAAA AACTCCCACT 
TCTCCAAAAA CTCTTGTGAA ATCGCACAGT TTGGCACAGC CTCCTGAGTT TGACAGTGGA 
GTTGAAACTT TCTCTATCCA TGCAGAGAAG CCTAAATATC AAATAAATAA TATCAGCACA 
GTGCCTAAAG CTATTCCTGT GAGTCCTTCA GCTGTGGAAG AGGATGAAGA TGAAGATGGT 
CATACTGTGG TGGCCACAGC CCGAGGCATA TTTAACAGCA ATGGGGGCGT GCTGAGTTCC 
ATAGAAACTG GCGTTAGTAT AATTATCCCT CAAGGAGCCA TTCCCGAAGG AGTTGAGCAG 
GAAATCTATT TCAAGGTCTG CCGGGACAAC AGCATCCTTC CACCTTTAGA TAAAGAGAAA 
GGTGAAACAC TGCTGAGTCC TTTGGTGATG TGTGGTCCCC ATGGCCTCAA GTTCCTGAAG 
CCTGTGGAGC TGCGCTTACC ACACTGTGCG TCCATGACTC CTGACGGTTG GTCTTTTGCT 
CTAAAATCAT CCGACTCCTC GTCGGGTGAT CCTAAAACCT GGCAAAACAA GTGTCTTCCC 
GGAGATCCAA ATTATCTCGT TGGAGCAAAC TGTGTTTCTG TCCTTATTGA CCACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016783332.1
CDS	89..4708
Translation	MKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQKAEASSPVPYLSPETNPASSTSAVNHNVNLTNVRLEEPTPAPSTSYSPQADSLRTPSTEAAHIMLRDQEPSLSSHVDPTKVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQPAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPELSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_016927843.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes tight junction protein 1 (TJP1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016927843.1

ORF Insert Sequence:

ATGAAGATAA ATGGTACTGT GACAGAAAAT ATGTCATTGA CAGATGCAAA GACATTGATA 
GAAAGGTCTA AAGGCAAATT AAAAATGGTA GTTCAAAGAG ATGAACGGGC TACGCTATTG 
AATGTCCCTG ATCTTTCTGA CAGCATCCAC TCTGCTAATG CCTCTGAGAG AGACGACATT 
TCAGAAATTC AGTCACTGGC ATCAGATCAT TCTGGTCGAT CACACGATAG GCCTCCCCGC 
CGCAGCCGGT CACGATCTCC TGACCAGCGG TCAGAGCCTT CTGATCATTC CAGGCACTCG 
CCACAGCAGC CAAGCAATGG CAGTCTCCGG AGTAGAGATG AAGAGAGAAT TTCTAAACCT 
GGGGCTGTCT CAACTCCTGT AAAGCATGCT GATGATCACA CACCTAAAAC AGTGGAAGAA 
GTTACAGTTG AAAGAAATGA GAAACAAACA CCTTCTCTTC CAGAACCAAA GCCTGTGTAT 
GCCCAAGTTG GGCAGCCAGA TGTGGATTTA CCTGTCAGTC CATCTGATGG TGTCCTACCT 
AATTCAACTC ATGAAGATGG GATTCTTCGG CCCAGCATGA AATTGGTAAA ATTCAGAAAA 
GGAGATAGTG TGGGTTTGCG GCTGGCTGGT GGAAATGATG TTGGAATATT TGTAGCTGGC 
GTTCTAGAAG ATAGCCCTGC AGCCAAGGAA GGCTTAGAGG AAGGTGATCA AATTCTCAGG 
GTAAACAACG TAGATTTTAC AAATATCATA AGAGAAGAAG CCGTCCTTTT CCTGCTTGAC 
CTCCCTAAAG GAGAAGAAGT GACCATATTG GCTCAGAAGA AGAAGGATGT TTATCGTCGC 
ATTGTAGAAT CAGATGTAGG AGATTCTTTC TATATTAGAA CCCATTTTGA ATATGAAAAG 
GAATCTCCCT ATGGACTTAG TTTTAACAAA GGAGAGGTGT TCCGTGTTGT GGATACCTTG 
TACAATGGAA AACTGGGCTC TTGGCTTGCT ATTCGAATTG GTAAAAATCA TAAGGAGGTA 
GAACGAGGCA TCATCCCTAA TAAGAACAGA GCTGAGCAGC TAGCCAGTGT ACAGTATACA 
CTTCCAAAAA CAGCAGGCGG AGACCGTGCT GACTTCTGGA GATTCAGAGG TCTTCGCAGC 
TCCAAGAGAA ATCTTCGAAA AAGCAGAGAG GATTTGTCCG CTCAGCCTGT TCAAACAAAG 
TTTCCAGCTT ATGAAAGAGT GGTTCTTCGA GAAGCTGGAT TTCTGAGGCC TGTAACCATT 
TTTGGACCAA TAGCTGATGT TGCCAGAGAA AAGCTGGCAA GAGAAGAACC AGATATTTAT 
CAAATTGCAA AGAGTGAACC ACGAGACGCT GGAACTGACC AACGTAGCTC TGGCATTATT 
CGCCTGCATA CAATAAAGCA AATCATAGAT CAAGACAAAC ATGCTTTATT AGATGTAACA 
CCAAATGCAG TTGATCGTCT TAACTATGCC CAGTGGTATC CAATTGTTGT ATTTCTTAAC 
CCTGATTCTA AGCAAGGAGT AAAAACAATG AGAATGAGGT TATGTCCAGA ATCTCGGAAA 
AGTGCCAGGA AGTTATATGA GCGATCTCAT AAACTTCGTA AAAATAATCA CCATCTTTTT 
ACAACTACAA TTAACTTAAA TTCAATGAAT GATGGTTGGT ATGGTGCGCT GAAAGAAGCA 
ATTCAACAAC AGCAAAACCA GCTGGTATGG GTTTCCGAGG GAAAGGCGGA TGGTGCTACA 
AGTGATGACC TTGATTTGCA TGATGATCGT CTGTCCTACC TGTCAGCTCC AGGTAGTGAA 
TACTCAATGT ATAGCACGGA CAGTAGACAC ACTTCTGACT ATGAAGACAC AGACACAGAA 
GGCGGGGCCT ACACTGATCA AGAACTAGAT GAAACTCTTA ATGATGAGGT TGGGACTCCA 
CCGGAGTCTG CCATTACACG GTCCTCTGAG CCTGTAAGAG AGGACTCCTC TGGAATGCAT 
CATGAAAACC AAACATATCC TCCTTACTCA CCACAAGCGC AGCCACAACC AATTCATAGA 
ATAGACTCCC CTGGATTTAA GCCAGCCTCT CAACAGAAAG CAGAAGCTTC ATCTCCAGTC 
CCTTACCTTT CGCCTGAAAC AAACCCAGCA TCATCAACCT CTGCTGTTAA TCATAATGTA 
AATTTAACTA ATGTCAGACT GGAGGAGCCC ACCCCAGCTC CTTCCACCTC TTACTCACCA 
CAAGCTGATT CTTTAAGAAC ACCAAGTACT GAGGCAGCTC ACATAATGCT AAGAGATCAA 
GAACCATCAT TGTCGTCGCA TGTAGATCCA ACAAAGGTGT ATAGAAAGGA TCCATATCCC 
GAGGAAATGA TGAGGCAGAA CCATGTTTTG AAACAGCCAG CCGTTAGTCA CCCAGGGCAC 
AGGCCAGACA AAGAGCCTAA TCTGACCTAT GAACCCCAAC TCCCATACGT AGAGAAACAA 
GCCAGCAGAG ACCTCGAGCA GCCCACATAC AGATACGAGT CCTCAAGCTA TACGGACCAG 
TTTTCTCGAA ACTATGAACA TCGTCTGCGA TACGAAGATC GCGTCCCCAT GTATGAAGAA 
CAGTGGTCAT ATTATGATGA CAAACAGCCC TACCCATCTC GGCCACCTTT TGATAATCAG 
CACTCTCAAG ACCTTGACTC CAGACAGCAT CCCGAAGAGT CCTCAGAACG AGGGTACTTT 
CCACGTTTTG AAGAGCCAGC CCCTCTGTCT TACGACAGCA GACCACGTTA CGAACAGGCA 
CCTAGAGCAT CCGCCCTGCG GCACGAAGAG CAGCCAGCTC CTGGGTATGA CACACATGGT 
AGACTCAGAC CGGAAGCCCA GCCCCACCCT TCAGCAGGGC CCAAGCCCGC AGAGTCCAAG 
CAGTATTTTG AGCAATATTC ACGCAGTTAC GAGCAAGTAC CACCCCAAGG ATTTACCTCT 
AGAGCAGGTC ATTTTGAGCC TCTCCATGGT GCTGCAGCTG TCCCTCCGCT GATACCTTCA 
TCTCAGCATA AGCCAGAAGC TCTGCCTTCA AACACCAAAC CACTGCCTCC ACCCCCAACT 
CAAACCGAAG AAGAGGAAGA TCCAGCAATG AAGCCACAGT CTGTACTCAC CAGAGTTAAG 
ATGTTTGAAA ACAAAAGATC TGCATCCTTA GAGACCAAGA AGGATGTAAA TGACACTGGC 
AGTTTTAAGC CTCCAGAAGT AGCATCTAAA CCTTCAGGTG CTCCCATCAT TGGTCCCAAA 
CCCACTTCTC AGAATCAGTT CAGTGAACAT GACAAAACTC TGTACAGGAT CCCAGAACCT 
CAAAAACCTC AACTGAAGCC ACCTGAAGAT ATCGTTCGGT CCAATCATTA TGACCCTGAA 
GAAGATGAAG AATATTATCG AAAACAGCTG TCATACTTTG ACCGAAGAAG TTTTGAGAAT 
AAGCCTCCTG CACACATTGC CGCCAGCCAT CTCTCCGAGC CTGCAAAGCC AGCTCATTCT 
CAGAATCAAT CAAATTTTTC TAGTTATTCT TCAAAGGGAA AGCCTCCTGA AGCTGATGGT 
GTGGATAGAT CATTTGGCGA GAAACGCTAT GAACCCATCC AGGCCACTCC CCCTCCTCCT 
CCATTGCCCT CGCAGTATGC CCAGCCATCT CAGCCTGTCA CCAGCGCGTC TCTCCACATA 
CATTCTAAGG GAGCACATGG TGAAGGTAAT TCAGTGTCAT TGGATTTTCA GAATTCCTTA 
GTGTCCAAAC CAGACCCACC TCCATCTCAG AATAAGCCAG CAACTTTCAG ACCACCAAAC 
CGAGAAGATA CTGCTCAGCC AGCTTTCTAT CCCCAGAAAA GTTTTCCAGA TAAAGCCCCA 
GTTAATGGAA CTGAACAGAC TCAGAAAACA GTCACTCCAG CATACAATCG ATTCACACCA 
AAACCATATA CAAGTTCTGC CCGACCATTT GAACGCAAGT TTGAAAGTCC TAAATTCAAT 
CACAATCTTC TGCCAAGTGA AACTGCACAT AAACCTGAAT TGTCTTCAAA AACTCCCACT 
TCTCCAAAAA CTCTTGTGAA ATCGCACAGT TTGGCACAGC CTCCTGAGTT TGACAGTGGA 
GTTGAAACTT TCTCTATCCA TGCAGAGAAG CCTAAATATC AAATAAATAA TATCAGCACA 
GTGCCTAAAG CTATTCCTGT GAGTCCTTCA GCTGTGGAAG AGGATGAAGA TGAAGATGGT 
CATACTGTGG TGGCCACAGC CCGAGGCATA TTTAACAGCA ATGGGGGCGT GCTGAGTTCC 
ATAGAAACTG GCGTTAGTAT AATTATCCCT CAAGGAGCCA TTCCCGAAGG AGTTGAGCAG 
GAAATCTATT TCAAGGTCTG CCGGGACAAC AGCATCCTTC CACCTTTAGA TAAAGAGAAA 
GGTGAAACAC TGCTGAGTCC TTTGGTGATG TGTGGTCCCC ATGGCCTCAA GTTCCTGAAG 
CCTGTGGAGC TGCGCTTACC ACACTGTGCG TCCATGACTC CTGACGGTTG GTCTTTTGCT 
CTAAAATCAT CCGACTCCTC GTCGGGTGAT CCTAAAACCT GGCAAAACAA GTGTCTTCCC 
GGAGATCCAA ATTATCTCGT TGGAGCAAAC TGTGTTTCTG TCCTTATTGA CCACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_009428684.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009426959.2 Pan troglodytes tight junction protein 1 (TJP1), transcript variant X1, mRNA.

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CloneID	OPc97826
Clone ID Related Accession (Same CDS sequence)	XM_009428684.3
Accession Version	XM_009428684.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5586bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	tight junction protein ZO-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036894.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009428684.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG 
CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA 
AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC 
AGGGCTCCTG GATTTGGGTT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA 
CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA 
AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT 
GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT 
AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG 
TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CCGCTAAACC TACTAAAGTC 
ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT 
GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT 
GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA 
TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG 
CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC 
GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT 
CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG 
CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT 
AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG 
GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT 
GTGTATGCCC AAGTTGGGCA GCCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC 
CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG 
CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT 
CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT 
GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT 
ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG 
TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA 
ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA 
ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT 
ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC 
ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT 
GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT 
CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT 
CGGAAAAGTG CCAGGAAGTT ATATGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT 
CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA 
GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT 
AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG 
ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA 
ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT 
CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCTTCATCT 
CCAGTCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAT CAACCTCTGC TGTTAATCAT 
AATGTAAATT TAACTAATGT CAGACTGGAG GAGCCCACCC CAGCTCCTTC CACCTCTTAC 
TCACCACAAG CTGATTCTTT AAGAACACCA AGTACTGAGG CAGCTCACAT AATGCTAAGA 
GATCAAGAAC CATCATTGTC GTCGCATGTA GATCCAACAA AGGTGTATAG AAAGGATCCA 
TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA 
GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG 
AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG 
GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT 
GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT 
AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG 
TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA 
CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA 
CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCCGCAGAG 
TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT 
ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA 
CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC 
CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA 
GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC 
ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT 
CCCAAACCCA CTTCTCAGAA TCAGTTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA 
GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATCG TTCGGTCCAA TCATTATGAC 
CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT 
GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCT 
CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT 
GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT 
CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC 
CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT 
TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA 
CCAAACCGAG AAGATACTGC TCAGCCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA 
GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC 
ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA 
TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGAATTGTC TTCAAAAACT 
CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC 
AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC 
AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA 
GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG 
AGTTCCATAG AAACTGGCGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT 
GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA 
GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC 
CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGCGTCCA TGACTCCTGA CGGTTGGTCT 
TTTGCTCTAA AATCATCCGA CTCCTCGTCG GGTGATCCTA AAACCTGGCA AAACAAGTGT 
CTTCCCGGAG ATCCAAATTA TCTCGTTGGA GCAAACTGTG TTTCTGTCCT TATTGACCAC 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009426959.2
CDS	86..5671
Translation	MRRLEGLCLRRRRRRRGPARERSGDKMKYQKYLTVLQMAIGVTPSNRGSLLPLKRKLWVTPSSENPNGATSSVSQGKPSLRRIKGRLHRSKSLDSMDFCELTSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQKAEASSPVPYLSPETNPASSTSAVNHNVNLTNVRLEEPTPAPSTSYSPQADSLRTPSTEAAHIMLRDQEPSLSSHVDPTKVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQPAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPELSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_009428684.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes tight junction protein 1 (TJP1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009428684.3

ORF Insert Sequence:

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG 
CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA 
AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC 
AGGGCTCCTG GATTTGGGTT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA 
CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA 
AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT 
GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT 
AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG 
TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CCGCTAAACC TACTAAAGTC 
ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT 
GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT 
GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA 
TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG 
CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC 
GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT 
CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG 
CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT 
AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG 
GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT 
GTGTATGCCC AAGTTGGGCA GCCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC 
CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG 
CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT 
CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT 
GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT 
ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG 
TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA 
ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA 
ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT 
ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC 
ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT 
GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT 
CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT 
CGGAAAAGTG CCAGGAAGTT ATATGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT 
CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA 
GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT 
AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG 
ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA 
ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT 
CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCTTCATCT 
CCAGTCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAT CAACCTCTGC TGTTAATCAT 
AATGTAAATT TAACTAATGT CAGACTGGAG GAGCCCACCC CAGCTCCTTC CACCTCTTAC 
TCACCACAAG CTGATTCTTT AAGAACACCA AGTACTGAGG CAGCTCACAT AATGCTAAGA 
GATCAAGAAC CATCATTGTC GTCGCATGTA GATCCAACAA AGGTGTATAG AAAGGATCCA 
TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA 
GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG 
AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG 
GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT 
GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT 
AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG 
TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA 
CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA 
CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCCGCAGAG 
TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT 
ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA 
CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC 
CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA 
GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC 
ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT 
CCCAAACCCA CTTCTCAGAA TCAGTTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA 
GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATCG TTCGGTCCAA TCATTATGAC 
CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT 
GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCT 
CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT 
GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT 
CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC 
CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT 
TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA 
CCAAACCGAG AAGATACTGC TCAGCCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA 
GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC 
ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA 
TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGAATTGTC TTCAAAAACT 
CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC 
AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC 
AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA 
GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG 
AGTTCCATAG AAACTGGCGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT 
GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA 
GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC 
CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGCGTCCA TGACTCCTGA CGGTTGGTCT 
TTTGCTCTAA AATCATCCGA CTCCTCGTCG GGTGATCCTA AAACCTGGCA AAACAAGTGT 
CTTCCCGGAG ATCCAAATTA TCTCGTTGGA GCAAACTGTG TTTCTGTCCT TATTGACCAC 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_009428685.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009426960.2 Pan troglodytes tight junction protein 1 (TJP1), transcript variant X2, mRNA.

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CloneID	OPc97827
Clone ID Related Accession (Same CDS sequence)	XM_009428685.3
Accession Version	XM_009428685.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5547bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	tight junction protein ZO-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036894.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009428685.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG 
CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA 
AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC 
AGGGCTCCTG GATTTGGGTT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA 
CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA 
AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT 
GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT 
AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG 
TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CCGCTAAACC TACTAAAGTC 
ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT 
GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT 
GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA 
TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG 
CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC 
GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT 
CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG 
CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT 
AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG 
GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT 
GTGTATGCCC AAGTTGGGCA GCCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC 
CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG 
CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT 
CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT 
GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT 
ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG 
TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA 
ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA 
ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT 
ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC 
ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT 
GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT 
CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT 
CGGAAAAGTG CCAGGAAGTT ATATGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT 
CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA 
GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT 
AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG 
ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA 
ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT 
CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCTTCATCT 
CCAGTCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAT CAACCTCTGC TGTTAATCAT 
AATGTAAATT TAACTAATGT CAGACTGGAG GAGCCCACCC CAGCTCCTTC CACCTCTTAC 
TCACCACAAG CTGATTCTTT AAGAACACCA AGTACTGAGG CAGCTCACAT AATGCTAAGA 
GATCAAGAAC CATCATTGTC GTCGCATGTA GATCCAACAA AGGTGTATAG AAAGGATCCA 
TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA 
GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG 
AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG 
GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT 
GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT 
AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG 
TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA 
CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA 
CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCCGCAGAG 
TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT 
ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA 
CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC 
CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA 
GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC 
ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT 
CCCAAACCCA CTTCTCAGAA TCAGTTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA 
GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATCG TTCGGTCCAA TCATTATGAC 
CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT 
GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCT 
CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT 
GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT 
CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC 
CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT 
TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA 
CCAAACCGAG AAGATACTGC TCAGCCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA 
GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC 
ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA 
TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGAATTGTC TTCAAAAACT 
CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC 
AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC 
AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA 
GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG 
AGTTCCATAG AAACTGGCGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT 
GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA 
GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC 
CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGCGTCCA TGACTCCTGA CGGTGATCCT 
AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT 
GTTTCTGTCC TTATTGACCA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009426960.2
CDS	17..5563
Translation	MRRLEGLCLRRRRRRRGPARERSGDKMKYQKYLTVLQMAIGVTPSNRGSLLPLKRKLWVTPSSENPNGATSSVSQGKPSLRRIKGRLHRSKSLDSMDFCELTSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQKAEASSPVPYLSPETNPASSTSAVNHNVNLTNVRLEEPTPAPSTSYSPQADSLRTPSTEAAHIMLRDQEPSLSSHVDPTKVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQPAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPELSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_009428685.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes tight junction protein 1 (TJP1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009428685.3

ORF Insert Sequence:

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG 
CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA 
AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC 
AGGGCTCCTG GATTTGGGTT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA 
CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA 
AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT 
GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT 
AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG 
TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CCGCTAAACC TACTAAAGTC 
ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT 
GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT 
GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA 
TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG 
CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC 
GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT 
CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG 
CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT 
AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG 
GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT 
GTGTATGCCC AAGTTGGGCA GCCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC 
CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG 
CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT 
CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT 
GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT 
ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG 
TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA 
ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA 
ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT 
ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC 
ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT 
GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT 
CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT 
CGGAAAAGTG CCAGGAAGTT ATATGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT 
CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA 
GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT 
AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG 
ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA 
ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT 
CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCTTCATCT 
CCAGTCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAT CAACCTCTGC TGTTAATCAT 
AATGTAAATT TAACTAATGT CAGACTGGAG GAGCCCACCC CAGCTCCTTC CACCTCTTAC 
TCACCACAAG CTGATTCTTT AAGAACACCA AGTACTGAGG CAGCTCACAT AATGCTAAGA 
GATCAAGAAC CATCATTGTC GTCGCATGTA GATCCAACAA AGGTGTATAG AAAGGATCCA 
TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA 
GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG 
AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG 
GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT 
GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT 
AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG 
TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA 
CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA 
CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCCGCAGAG 
TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT 
ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA 
CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC 
CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA 
GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC 
ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT 
CCCAAACCCA CTTCTCAGAA TCAGTTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA 
GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATCG TTCGGTCCAA TCATTATGAC 
CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT 
GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCT 
CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT 
GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT 
CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC 
CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT 
TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA 
CCAAACCGAG AAGATACTGC TCAGCCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA 
GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC 
ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA 
TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGAATTGTC TTCAAAAACT 
CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC 
AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC 
AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA 
GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG 
AGTTCCATAG AAACTGGCGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT 
GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA 
GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC 
CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGCGTCCA TGACTCCTGA CGGTGATCCT 
AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT 
GTTTCTGTCC TTATTGACCA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_009428686.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009426961.2 Pan troglodytes tight junction protein 1 (TJP1), transcript variant X3, mRNA.

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CloneID	OPc97828
Clone ID Related Accession (Same CDS sequence)	XM_009428686.3
Accession Version	XM_009428686.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5526bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	tight junction protein ZO-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036894.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009428686.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG 
CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA 
AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC 
AGGGCTCCTG GATTTGGGTT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA 
CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA 
AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT 
GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT 
AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG 
TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CCGCTAAACC TACTAAAGTC 
ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT 
GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT 
GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA 
TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG 
CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC 
GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT 
CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG 
CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT 
AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG 
GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT 
GTGTATGCCC AAGTTGGGCA GCCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC 
CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG 
CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT 
CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT 
GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT 
ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG 
TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA 
ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA 
ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT 
ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC 
ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT 
GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT 
CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT 
CGGAAAAGTG CCAGGAAGTT ATATGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT 
CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA 
GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT 
AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG 
ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA 
ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT 
CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCTTCATCT 
CCAGTCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAT CAACCTCTGC TGTTAATCAT 
AATGTAAATT TAACTAATGT CAGACTGGAG GAGCCCACCC CAGCTCCTTC CACCTCTTAC 
TCACCACAAG CTGATTCTTT AAGAACACCA AGTACTGAGG CAGCTCACAT AATGCTAAGA 
GATCAAGAAC CATCATTGTC GTCGCATGTA GATCCAACAA AGGTGTATAG AAAGGATCCA 
TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA 
GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG 
AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG 
GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT 
GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT 
AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG 
TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA 
CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA 
CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCCGCAGAG 
TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT 
ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA 
CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC 
CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA 
GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC 
ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT 
CCCAAACCCA CTTCTCAGAA TCAGTTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA 
GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATCG TTCGGTCCAA TCATTATGAC 
CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT 
GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCT 
CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT 
GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT 
CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC 
CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT 
TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA 
CCAAACCGAG AAGATACTGC TCAGCCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA 
GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC 
ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA 
TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGAATTGTC TTCAAAAACT 
CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC 
AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC 
AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA 
GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG 
AGTTCCATAG AAACTGGCGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT 
GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA 
GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC 
CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGATCCTA AAACCTGGCA AAACAAGTGT 
CTTCCCGGAG ATCCAAATTA TCTCGTTGGA GCAAACTGTG TTTCTGTCCT TATTGACCAC 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009426961.2
CDS	86..5611
Translation	MRRLEGLCLRRRRRRRGPARERSGDKMKYQKYLTVLQMAIGVTPSNRGSLLPLKRKLWVTPSSENPNGATSSVSQGKPSLRRIKGRLHRSKSLDSMDFCELTSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQKAEASSPVPYLSPETNPASSTSAVNHNVNLTNVRLEEPTPAPSTSYSPQADSLRTPSTEAAHIMLRDQEPSLSSHVDPTKVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQPAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPELSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_009428686.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes tight junction protein 1 (TJP1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009428686.3

ORF Insert Sequence:

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG 
CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA 
AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC 
AGGGCTCCTG GATTTGGGTT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA 
CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA 
AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT 
GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT 
AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG 
TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CCGCTAAACC TACTAAAGTC 
ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT 
GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT 
GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA 
TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG 
CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC 
GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT 
CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG 
CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT 
AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG 
GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT 
GTGTATGCCC AAGTTGGGCA GCCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC 
CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG 
CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT 
CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT 
GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT 
ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG 
TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA 
ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA 
ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT 
ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC 
ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT 
GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT 
CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT 
CGGAAAAGTG CCAGGAAGTT ATATGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT 
CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA 
GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT 
AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG 
ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA 
ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT 
CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCTTCATCT 
CCAGTCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAT CAACCTCTGC TGTTAATCAT 
AATGTAAATT TAACTAATGT CAGACTGGAG GAGCCCACCC CAGCTCCTTC CACCTCTTAC 
TCACCACAAG CTGATTCTTT AAGAACACCA AGTACTGAGG CAGCTCACAT AATGCTAAGA 
GATCAAGAAC CATCATTGTC GTCGCATGTA GATCCAACAA AGGTGTATAG AAAGGATCCA 
TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA 
GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG 
AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG 
GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT 
GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT 
AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG 
TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA 
CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA 
CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCCGCAGAG 
TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT 
ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA 
CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC 
CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA 
GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC 
ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT 
CCCAAACCCA CTTCTCAGAA TCAGTTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA 
GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATCG TTCGGTCCAA TCATTATGAC 
CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT 
GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCT 
CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT 
GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT 
CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC 
CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT 
TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA 
CCAAACCGAG AAGATACTGC TCAGCCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA 
GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC 
ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA 
TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGAATTGTC TTCAAAAACT 
CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC 
AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC 
AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA 
GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG 
AGTTCCATAG AAACTGGCGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT 
GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA 
GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC 
CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGATCCTA AAACCTGGCA AAACAAGTGT 
CTTCCCGGAG ATCCAAATTA TCTCGTTGGA GCAAACTGTG TTTCTGTCCT TATTGACCAC 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_009428689.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009426964.2 Pan troglodytes tight junction protein 1 (TJP1), transcript variant X4, mRNA.

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CloneID	OPc97829
Clone ID Related Accession (Same CDS sequence)	XM_009428689.3
Accession Version	XM_009428689.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5346bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	tight junction protein ZO-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036894.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009428689.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG 
CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA 
AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC 
AGGGCTCCTG GATTTGGGTT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA 
CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA 
AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT 
GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT 
AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG 
TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CCGCTAAACC TACTAAAGTC 
ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT 
GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT 
GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA 
TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG 
CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC 
GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT 
CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG 
CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT 
AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG 
GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT 
GTGTATGCCC AAGTTGGGCA GCCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC 
CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG 
CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT 
CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT 
GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT 
ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG 
TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA 
ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA 
ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT 
ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC 
ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT 
GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT 
CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT 
CGGAAAAGTG CCAGGAAGTT ATATGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT 
CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA 
GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT 
AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG 
ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA 
ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT 
CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGGTGTATAG AAAGGATCCA 
TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA 
GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG 
AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG 
GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT 
GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT 
AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG 
TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA 
CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA 
CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCCGCAGAG 
TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT 
ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA 
CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC 
CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA 
GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC 
ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT 
CCCAAACCCA CTTCTCAGAA TCAGTTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA 
GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATCG TTCGGTCCAA TCATTATGAC 
CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT 
GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCT 
CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT 
GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT 
CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC 
CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT 
TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA 
CCAAACCGAG AAGATACTGC TCAGCCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA 
GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC 
ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA 
TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGAATTGTC TTCAAAAACT 
CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC 
AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC 
AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA 
GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG 
AGTTCCATAG AAACTGGCGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT 
GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA 
GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC 
CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGCGTCCA TGACTCCTGA CGGTTGGTCT 
TTTGCTCTAA AATCATCCGA CTCCTCGTCG GGTGATCCTA AAACCTGGCA AAACAAGTGT 
CTTCCCGGAG ATCCAAATTA TCTCGTTGGA GCAAACTGTG TTTCTGTCCT TATTGACCAC 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009426964.2
CDS	16..5361
Translation	MRRLEGLCLRRRRRRRGPARERSGDKMKYQKYLTVLQMAIGVTPSNRGSLLPLKRKLWVTPSSENPNGATSSVSQGKPSLRRIKGRLHRSKSLDSMDFCELTSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQPAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPELSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_009428689.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes tight junction protein 1 (TJP1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009428689.3

ORF Insert Sequence:

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG 
CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA 
AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC 
AGGGCTCCTG GATTTGGGTT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA 
CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA 
AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT 
GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT 
AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG 
TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CCGCTAAACC TACTAAAGTC 
ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT 
GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT 
GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA 
TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG 
CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC 
GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT 
CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG 
CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT 
AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG 
GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT 
GTGTATGCCC AAGTTGGGCA GCCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC 
CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG 
CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT 
CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT 
GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT 
ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG 
TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA 
ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA 
ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT 
ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC 
ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT 
GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT 
CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT 
CGGAAAAGTG CCAGGAAGTT ATATGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT 
CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA 
GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT 
AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG 
ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA 
ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT 
CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGGTGTATAG AAAGGATCCA 
TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA 
GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG 
AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG 
GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT 
GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT 
AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG 
TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA 
CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA 
CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCCGCAGAG 
TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT 
ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA 
CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC 
CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA 
GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC 
ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT 
CCCAAACCCA CTTCTCAGAA TCAGTTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA 
GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATCG TTCGGTCCAA TCATTATGAC 
CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT 
GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCT 
CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT 
GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT 
CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC 
CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT 
TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA 
CCAAACCGAG AAGATACTGC TCAGCCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA 
GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC 
ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA 
TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGAATTGTC TTCAAAAACT 
CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC 
AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC 
AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA 
GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG 
AGTTCCATAG AAACTGGCGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT 
GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA 
GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC 
CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGCGTCCA TGACTCCTGA CGGTTGGTCT 
TTTGCTCTAA AATCATCCGA CTCCTCGTCG GGTGATCCTA AAACCTGGCA AAACAAGTGT 
CTTCCCGGAG ATCCAAATTA TCTCGTTGGA GCAAACTGTG TTTCTGTCCT TATTGACCAC 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_009428687.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009426962.2 Pan troglodytes tight junction protein 1 (TJP1), transcript variant X5, mRNA.

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CloneID	OPc97830
Clone ID Related Accession (Same CDS sequence)	XM_009428687.3
Accession Version	XM_009428687.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5319bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	tight junction protein ZO-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036894.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009428687.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGAA ATACGTTTTC AATGGATTGC CACAGTAAGA GCACAGCAAT GGAGGAAACA 
GCTATATGGG AACAACATAC AGTGACGCTT CACAGGGCTC CTGGATTTGG GTTTGGAATT 
GCAATATCTG GTGGACGAGA TAATCCTCAT TTTCAGAGTG GGGAAACGTC AATAGTGATT 
TCAGATGTGC TGAAAGGAGG ACCAGCTGAA GGACAGCTAC AGGAAAATGA CCGAGTTGCA 
ATGGTTAACG GAGTTTCAAT GGATAATGTT GAACATGCTT TTGCTGTTCA GCAACTAAGG 
AAAAGTGGGA AAAATGCAAA AATTACAATT AGAAGGAAGA AGAAAGTTCA AATACCAGTA 
AGTCGTCCTG ATCCTGAACC AGTATCTGAT AATGAAGAAG ATAGTTATGA TGAGGAAATA 
CATGATCCAA GAAGTGGCCG GAGTGGTGTG GTTAACAGAA GGAGTGAGAA GATTTGGCCG 
AGGGATAGAA GTGCAAGTAG AGAGAGGAGC TTGTCCCCGC GGTCAGACAG GCGGTCAGTG 
GCTTCCAGCC AGCCCGCTAA ACCTACTAAA GTCACACTGG TGAAATCCCG GAAAAATGAA 
GAATATGGTC TTCGATTGGC AAGCCATATA TTTGTTAAGG AAATTTCACA AGATAGTTTG 
GCAGCAAGAG ATGGCAATAT TCAAGAAGGT GATGTTGTAT TGAAGATAAA TGGTACTGTG 
ACAGAAAATA TGTCATTGAC AGATGCAAAG ACATTGATAG AAAGGTCTAA AGGCAAATTA 
AAAATGGTAG TTCAAAGAGA TGAACGGGCT ACGCTATTGA ATGTCCCTGA TCTTTCTGAC 
AGCATCCACT CTGCTAATGC CTCTGAGAGA GACGACATTT CAGAAATTCA GTCACTGGCA 
TCAGATCATT CTGGTCGATC ACACGATAGG CCTCCCCGCC GCAGCCGGTC ACGATCTCCT 
GACCAGCGGT CAGAGCCTTC TGATCATTCC AGGCACTCGC CGCAGCAGCC AAGCAATGGC 
AGTCTCCGGA GTAGAGATGA AGAGAGAATT TCTAAACCTG GGGCTGTCTC AACTCCTGTA 
AAGCATGCTG ATGATCACAC ACCTAAAACA GTGGAAGAAG TTACAGTTGA AAGAAATGAG 
AAACAAACAC CTTCTCTTCC AGAACCAAAG CCTGTGTATG CCCAAGTTGG GCAGCCAGAT 
GTGGATTTAC CTGTCAGTCC ATCTGATGGT GTCCTACCTA ATTCAACTCA TGAAGATGGG 
ATTCTTCGGC CCAGCATGAA ATTGGTAAAA TTCAGAAAAG GAGATAGTGT GGGTTTGCGG 
CTGGCTGGTG GAAATGATGT TGGAATATTT GTAGCTGGCG TTCTAGAAGA TAGCCCTGCA 
GCCAAGGAAG GCTTAGAGGA AGGTGATCAA ATTCTCAGGG TAAACAACGT AGATTTTACA 
AATATCATAA GAGAAGAAGC CGTCCTTTTC CTGCTTGACC TCCCTAAAGG AGAAGAAGTG 
ACCATATTGG CTCAGAAGAA GAAGGATGTT TATCGTCGCA TTGTAGAATC AGATGTAGGA 
GATTCTTTCT ATATTAGAAC CCATTTTGAA TATGAAAAGG AATCTCCCTA TGGACTTAGT 
TTTAACAAAG GAGAGGTGTT CCGTGTTGTG GATACCTTGT ACAATGGAAA ACTGGGCTCT 
TGGCTTGCTA TTCGAATTGG TAAAAATCAT AAGGAGGTAG AACGAGGCAT CATCCCTAAT 
AAGAACAGAG CTGAGCAGCT AGCCAGTGTA CAGTATACAC TTCCAAAAAC AGCAGGCGGA 
GACCGTGCTG ACTTCTGGAG ATTCAGAGGT CTTCGCAGCT CCAAGAGAAA TCTTCGAAAA 
AGCAGAGAGG ATTTGTCCGC TCAGCCTGTT CAAACAAAGT TTCCAGCTTA TGAAAGAGTG 
GTTCTTCGAG AAGCTGGATT TCTGAGGCCT GTAACCATTT TTGGACCAAT AGCTGATGTT 
GCCAGAGAAA AGCTGGCAAG AGAAGAACCA GATATTTATC AAATTGCAAA GAGTGAACCA 
CGAGACGCTG GAACTGACCA ACGTAGCTCT GGCATTATTC GCCTGCATAC AATAAAGCAA 
ATCATAGATC AAGACAAACA TGCTTTATTA GATGTAACAC CAAATGCAGT TGATCGTCTT 
AACTATGCCC AGTGGTATCC AATTGTTGTA TTTCTTAACC CTGATTCTAA GCAAGGAGTA 
AAAACAATGA GAATGAGGTT ATGTCCAGAA TCTCGGAAAA GTGCCAGGAA GTTATATGAG 
CGATCTCATA AACTTCGTAA AAATAATCAC CATCTTTTTA CAACTACAAT TAACTTAAAT 
TCAATGAATG ATGGTTGGTA TGGTGCGCTG AAAGAAGCAA TTCAACAACA GCAAAACCAG 
CTGGTATGGG TTTCCGAGGG AAAGGCGGAT GGTGCTACAA GTGATGACCT TGATTTGCAT 
GATGATCGTC TGTCCTACCT GTCAGCTCCA GGTAGTGAAT ACTCAATGTA TAGCACGGAC 
AGTAGACACA CTTCTGACTA TGAAGACACA GACACAGAAG GCGGGGCCTA CACTGATCAA 
GAACTAGATG AAACTCTTAA TGATGAGGTT GGGACTCCAC CGGAGTCTGC CATTACACGG 
TCCTCTGAGC CTGTAAGAGA GGACTCCTCT GGAATGCATC ATGAAAACCA AACATATCCT 
CCTTACTCAC CACAAGCGCA GCCACAACCA ATTCATAGAA TAGACTCCCC TGGATTTAAG 
CCAGCCTCTC AACAGAAAGC AGAAGCTTCA TCTCCAGTCC CTTACCTTTC GCCTGAAACA 
AACCCAGCAT CATCAACCTC TGCTGTTAAT CATAATGTAA ATTTAACTAA TGTCAGACTG 
GAGGAGCCCA CCCCAGCTCC TTCCACCTCT TACTCACCAC AAGCTGATTC TTTAAGAACA 
CCAAGTACTG AGGCAGCTCA CATAATGCTA AGAGATCAAG AACCATCATT GTCGTCGCAT 
GTAGATCCAA CAAAGGTGTA TAGAAAGGAT CCATATCCCG AGGAAATGAT GAGGCAGAAC 
CATGTTTTGA AACAGCCAGC CGTTAGTCAC CCAGGGCACA GGCCAGACAA AGAGCCTAAT 
CTGACCTATG AACCCCAACT CCCATACGTA GAGAAACAAG CCAGCAGAGA CCTCGAGCAG 
CCCACATACA GATACGAGTC CTCAAGCTAT ACGGACCAGT TTTCTCGAAA CTATGAACAT 
CGTCTGCGAT ACGAAGATCG CGTCCCCATG TATGAAGAAC AGTGGTCATA TTATGATGAC 
AAACAGCCCT ACCCATCTCG GCCACCTTTT GATAATCAGC ACTCTCAAGA CCTTGACTCC 
AGACAGCATC CCGAAGAGTC CTCAGAACGA GGGTACTTTC CACGTTTTGA AGAGCCAGCC 
CCTCTGTCTT ACGACAGCAG ACCACGTTAC GAACAGGCAC CTAGAGCATC CGCCCTGCGG 
CACGAAGAGC AGCCAGCTCC TGGGTATGAC ACACATGGTA GACTCAGACC GGAAGCCCAG 
CCCCACCCTT CAGCAGGGCC CAAGCCCGCA GAGTCCAAGC AGTATTTTGA GCAATATTCA 
CGCAGTTACG AGCAAGTACC ACCCCAAGGA TTTACCTCTA GAGCAGGTCA TTTTGAGCCT 
CTCCATGGTG CTGCAGCTGT CCCTCCGCTG ATACCTTCAT CTCAGCATAA GCCAGAAGCT 
CTGCCTTCAA ACACCAAACC ACTGCCTCCA CCCCCAACTC AAACCGAAGA AGAGGAAGAT 
CCAGCAATGA AGCCACAGTC TGTACTCACC AGAGTTAAGA TGTTTGAAAA CAAAAGATCT 
GCATCCTTAG AGACCAAGAA GGATGTAAAT GACACTGGCA GTTTTAAGCC TCCAGAAGTA 
GCATCTAAAC CTTCAGGTGC TCCCATCATT GGTCCCAAAC CCACTTCTCA GAATCAGTTC 
AGTGAACATG ACAAAACTCT GTACAGGATC CCAGAACCTC AAAAACCTCA ACTGAAGCCA 
CCTGAAGATA TCGTTCGGTC CAATCATTAT GACCCTGAAG AAGATGAAGA ATATTATCGA 
AAACAGCTGT CATACTTTGA CCGAAGAAGT TTTGAGAATA AGCCTCCTGC ACACATTGCC 
GCCAGCCATC TCTCCGAGCC TGCAAAGCCA GCTCATTCTC AGAATCAATC AAATTTTTCT 
AGTTATTCTT CAAAGGGAAA GCCTCCTGAA GCTGATGGTG TGGATAGATC ATTTGGCGAG 
AAACGCTATG AACCCATCCA GGCCACTCCC CCTCCTCCTC CATTGCCCTC GCAGTATGCC 
CAGCCATCTC AGCCTGTCAC CAGCGCGTCT CTCCACATAC ATTCTAAGGG AGCACATGGT 
GAAGGTAATT CAGTGTCATT GGATTTTCAG AATTCCTTAG TGTCCAAACC AGACCCACCT 
CCATCTCAGA ATAAGCCAGC AACTTTCAGA CCACCAAACC GAGAAGATAC TGCTCAGCCA 
GCTTTCTATC CCCAGAAAAG TTTTCCAGAT AAAGCCCCAG TTAATGGAAC TGAACAGACT 
CAGAAAACAG TCACTCCAGC ATACAATCGA TTCACACCAA AACCATATAC AAGTTCTGCC 
CGACCATTTG AACGCAAGTT TGAAAGTCCT AAATTCAATC ACAATCTTCT GCCAAGTGAA 
ACTGCACATA AACCTGAATT GTCTTCAAAA ACTCCCACTT CTCCAAAAAC TCTTGTGAAA 
TCGCACAGTT TGGCACAGCC TCCTGAGTTT GACAGTGGAG TTGAAACTTT CTCTATCCAT 
GCAGAGAAGC CTAAATATCA AATAAATAAT ATCAGCACAG TGCCTAAAGC TATTCCTGTG 
AGTCCTTCAG CTGTGGAAGA GGATGAAGAT GAAGATGGTC ATACTGTGGT GGCCACAGCC 
CGAGGCATAT TTAACAGCAA TGGGGGCGTG CTGAGTTCCA TAGAAACTGG CGTTAGTATA 
ATTATCCCTC AAGGAGCCAT TCCCGAAGGA GTTGAGCAGG AAATCTATTT CAAGGTCTGC 
CGGGACAACA GCATCCTTCC ACCTTTAGAT AAAGAGAAAG GTGAAACACT GCTGAGTCCT 
TTGGTGATGT GTGGTCCCCA TGGCCTCAAG TTCCTGAAGC CTGTGGAGCT GCGCTTACCA 
CACTGTGCGT CCATGACTCC TGACGGTTGG TCTTTTGCTC TAAAATCATC CGACTCCTCG 
TCGGGTGATC CTAAAACCTG GCAAAACAAG TGTCTTCCCG GAGATCCAAA TTATCTCGTT 
GGAGCAAACT GTGTTTCTGT CCTTATTGAC CACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009426962.2
CDS	110..5428
Translation	MERNTFSMDCHSKSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQKAEASSPVPYLSPETNPASSTSAVNHNVNLTNVRLEEPTPAPSTSYSPQADSLRTPSTEAAHIMLRDQEPSLSSHVDPTKVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQPAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPELSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_009428687.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes tight junction protein 1 (TJP1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009428687.3

ORF Insert Sequence:

ATGGAGAGAA ATACGTTTTC AATGGATTGC CACAGTAAGA GCACAGCAAT GGAGGAAACA 
GCTATATGGG AACAACATAC AGTGACGCTT CACAGGGCTC CTGGATTTGG GTTTGGAATT 
GCAATATCTG GTGGACGAGA TAATCCTCAT TTTCAGAGTG GGGAAACGTC AATAGTGATT 
TCAGATGTGC TGAAAGGAGG ACCAGCTGAA GGACAGCTAC AGGAAAATGA CCGAGTTGCA 
ATGGTTAACG GAGTTTCAAT GGATAATGTT GAACATGCTT TTGCTGTTCA GCAACTAAGG 
AAAAGTGGGA AAAATGCAAA AATTACAATT AGAAGGAAGA AGAAAGTTCA AATACCAGTA 
AGTCGTCCTG ATCCTGAACC AGTATCTGAT AATGAAGAAG ATAGTTATGA TGAGGAAATA 
CATGATCCAA GAAGTGGCCG GAGTGGTGTG GTTAACAGAA GGAGTGAGAA GATTTGGCCG 
AGGGATAGAA GTGCAAGTAG AGAGAGGAGC TTGTCCCCGC GGTCAGACAG GCGGTCAGTG 
GCTTCCAGCC AGCCCGCTAA ACCTACTAAA GTCACACTGG TGAAATCCCG GAAAAATGAA 
GAATATGGTC TTCGATTGGC AAGCCATATA TTTGTTAAGG AAATTTCACA AGATAGTTTG 
GCAGCAAGAG ATGGCAATAT TCAAGAAGGT GATGTTGTAT TGAAGATAAA TGGTACTGTG 
ACAGAAAATA TGTCATTGAC AGATGCAAAG ACATTGATAG AAAGGTCTAA AGGCAAATTA 
AAAATGGTAG TTCAAAGAGA TGAACGGGCT ACGCTATTGA ATGTCCCTGA TCTTTCTGAC 
AGCATCCACT CTGCTAATGC CTCTGAGAGA GACGACATTT CAGAAATTCA GTCACTGGCA 
TCAGATCATT CTGGTCGATC ACACGATAGG CCTCCCCGCC GCAGCCGGTC ACGATCTCCT 
GACCAGCGGT CAGAGCCTTC TGATCATTCC AGGCACTCGC CGCAGCAGCC AAGCAATGGC 
AGTCTCCGGA GTAGAGATGA AGAGAGAATT TCTAAACCTG GGGCTGTCTC AACTCCTGTA 
AAGCATGCTG ATGATCACAC ACCTAAAACA GTGGAAGAAG TTACAGTTGA AAGAAATGAG 
AAACAAACAC CTTCTCTTCC AGAACCAAAG CCTGTGTATG CCCAAGTTGG GCAGCCAGAT 
GTGGATTTAC CTGTCAGTCC ATCTGATGGT GTCCTACCTA ATTCAACTCA TGAAGATGGG 
ATTCTTCGGC CCAGCATGAA ATTGGTAAAA TTCAGAAAAG GAGATAGTGT GGGTTTGCGG 
CTGGCTGGTG GAAATGATGT TGGAATATTT GTAGCTGGCG TTCTAGAAGA TAGCCCTGCA 
GCCAAGGAAG GCTTAGAGGA AGGTGATCAA ATTCTCAGGG TAAACAACGT AGATTTTACA 
AATATCATAA GAGAAGAAGC CGTCCTTTTC CTGCTTGACC TCCCTAAAGG AGAAGAAGTG 
ACCATATTGG CTCAGAAGAA GAAGGATGTT TATCGTCGCA TTGTAGAATC AGATGTAGGA 
GATTCTTTCT ATATTAGAAC CCATTTTGAA TATGAAAAGG AATCTCCCTA TGGACTTAGT 
TTTAACAAAG GAGAGGTGTT CCGTGTTGTG GATACCTTGT ACAATGGAAA ACTGGGCTCT 
TGGCTTGCTA TTCGAATTGG TAAAAATCAT AAGGAGGTAG AACGAGGCAT CATCCCTAAT 
AAGAACAGAG CTGAGCAGCT AGCCAGTGTA CAGTATACAC TTCCAAAAAC AGCAGGCGGA 
GACCGTGCTG ACTTCTGGAG ATTCAGAGGT CTTCGCAGCT CCAAGAGAAA TCTTCGAAAA 
AGCAGAGAGG ATTTGTCCGC TCAGCCTGTT CAAACAAAGT TTCCAGCTTA TGAAAGAGTG 
GTTCTTCGAG AAGCTGGATT TCTGAGGCCT GTAACCATTT TTGGACCAAT AGCTGATGTT 
GCCAGAGAAA AGCTGGCAAG AGAAGAACCA GATATTTATC AAATTGCAAA GAGTGAACCA 
CGAGACGCTG GAACTGACCA ACGTAGCTCT GGCATTATTC GCCTGCATAC AATAAAGCAA 
ATCATAGATC AAGACAAACA TGCTTTATTA GATGTAACAC CAAATGCAGT TGATCGTCTT 
AACTATGCCC AGTGGTATCC AATTGTTGTA TTTCTTAACC CTGATTCTAA GCAAGGAGTA 
AAAACAATGA GAATGAGGTT ATGTCCAGAA TCTCGGAAAA GTGCCAGGAA GTTATATGAG 
CGATCTCATA AACTTCGTAA AAATAATCAC CATCTTTTTA CAACTACAAT TAACTTAAAT 
TCAATGAATG ATGGTTGGTA TGGTGCGCTG AAAGAAGCAA TTCAACAACA GCAAAACCAG 
CTGGTATGGG TTTCCGAGGG AAAGGCGGAT GGTGCTACAA GTGATGACCT TGATTTGCAT 
GATGATCGTC TGTCCTACCT GTCAGCTCCA GGTAGTGAAT ACTCAATGTA TAGCACGGAC 
AGTAGACACA CTTCTGACTA TGAAGACACA GACACAGAAG GCGGGGCCTA CACTGATCAA 
GAACTAGATG AAACTCTTAA TGATGAGGTT GGGACTCCAC CGGAGTCTGC CATTACACGG 
TCCTCTGAGC CTGTAAGAGA GGACTCCTCT GGAATGCATC ATGAAAACCA AACATATCCT 
CCTTACTCAC CACAAGCGCA GCCACAACCA ATTCATAGAA TAGACTCCCC TGGATTTAAG 
CCAGCCTCTC AACAGAAAGC AGAAGCTTCA TCTCCAGTCC CTTACCTTTC GCCTGAAACA 
AACCCAGCAT CATCAACCTC TGCTGTTAAT CATAATGTAA ATTTAACTAA TGTCAGACTG 
GAGGAGCCCA CCCCAGCTCC TTCCACCTCT TACTCACCAC AAGCTGATTC TTTAAGAACA 
CCAAGTACTG AGGCAGCTCA CATAATGCTA AGAGATCAAG AACCATCATT GTCGTCGCAT 
GTAGATCCAA CAAAGGTGTA TAGAAAGGAT CCATATCCCG AGGAAATGAT GAGGCAGAAC 
CATGTTTTGA AACAGCCAGC CGTTAGTCAC CCAGGGCACA GGCCAGACAA AGAGCCTAAT 
CTGACCTATG AACCCCAACT CCCATACGTA GAGAAACAAG CCAGCAGAGA CCTCGAGCAG 
CCCACATACA GATACGAGTC CTCAAGCTAT ACGGACCAGT TTTCTCGAAA CTATGAACAT 
CGTCTGCGAT ACGAAGATCG CGTCCCCATG TATGAAGAAC AGTGGTCATA TTATGATGAC 
AAACAGCCCT ACCCATCTCG GCCACCTTTT GATAATCAGC ACTCTCAAGA CCTTGACTCC 
AGACAGCATC CCGAAGAGTC CTCAGAACGA GGGTACTTTC CACGTTTTGA AGAGCCAGCC 
CCTCTGTCTT ACGACAGCAG ACCACGTTAC GAACAGGCAC CTAGAGCATC CGCCCTGCGG 
CACGAAGAGC AGCCAGCTCC TGGGTATGAC ACACATGGTA GACTCAGACC GGAAGCCCAG 
CCCCACCCTT CAGCAGGGCC CAAGCCCGCA GAGTCCAAGC AGTATTTTGA GCAATATTCA 
CGCAGTTACG AGCAAGTACC ACCCCAAGGA TTTACCTCTA GAGCAGGTCA TTTTGAGCCT 
CTCCATGGTG CTGCAGCTGT CCCTCCGCTG ATACCTTCAT CTCAGCATAA GCCAGAAGCT 
CTGCCTTCAA ACACCAAACC ACTGCCTCCA CCCCCAACTC AAACCGAAGA AGAGGAAGAT 
CCAGCAATGA AGCCACAGTC TGTACTCACC AGAGTTAAGA TGTTTGAAAA CAAAAGATCT 
GCATCCTTAG AGACCAAGAA GGATGTAAAT GACACTGGCA GTTTTAAGCC TCCAGAAGTA 
GCATCTAAAC CTTCAGGTGC TCCCATCATT GGTCCCAAAC CCACTTCTCA GAATCAGTTC 
AGTGAACATG ACAAAACTCT GTACAGGATC CCAGAACCTC AAAAACCTCA ACTGAAGCCA 
CCTGAAGATA TCGTTCGGTC CAATCATTAT GACCCTGAAG AAGATGAAGA ATATTATCGA 
AAACAGCTGT CATACTTTGA CCGAAGAAGT TTTGAGAATA AGCCTCCTGC ACACATTGCC 
GCCAGCCATC TCTCCGAGCC TGCAAAGCCA GCTCATTCTC AGAATCAATC AAATTTTTCT 
AGTTATTCTT CAAAGGGAAA GCCTCCTGAA GCTGATGGTG TGGATAGATC ATTTGGCGAG 
AAACGCTATG AACCCATCCA GGCCACTCCC CCTCCTCCTC CATTGCCCTC GCAGTATGCC 
CAGCCATCTC AGCCTGTCAC CAGCGCGTCT CTCCACATAC ATTCTAAGGG AGCACATGGT 
GAAGGTAATT CAGTGTCATT GGATTTTCAG AATTCCTTAG TGTCCAAACC AGACCCACCT 
CCATCTCAGA ATAAGCCAGC AACTTTCAGA CCACCAAACC GAGAAGATAC TGCTCAGCCA 
GCTTTCTATC CCCAGAAAAG TTTTCCAGAT AAAGCCCCAG TTAATGGAAC TGAACAGACT 
CAGAAAACAG TCACTCCAGC ATACAATCGA TTCACACCAA AACCATATAC AAGTTCTGCC 
CGACCATTTG AACGCAAGTT TGAAAGTCCT AAATTCAATC ACAATCTTCT GCCAAGTGAA 
ACTGCACATA AACCTGAATT GTCTTCAAAA ACTCCCACTT CTCCAAAAAC TCTTGTGAAA 
TCGCACAGTT TGGCACAGCC TCCTGAGTTT GACAGTGGAG TTGAAACTTT CTCTATCCAT 
GCAGAGAAGC CTAAATATCA AATAAATAAT ATCAGCACAG TGCCTAAAGC TATTCCTGTG 
AGTCCTTCAG CTGTGGAAGA GGATGAAGAT GAAGATGGTC ATACTGTGGT GGCCACAGCC 
CGAGGCATAT TTAACAGCAA TGGGGGCGTG CTGAGTTCCA TAGAAACTGG CGTTAGTATA 
ATTATCCCTC AAGGAGCCAT TCCCGAAGGA GTTGAGCAGG AAATCTATTT CAAGGTCTGC 
CGGGACAACA GCATCCTTCC ACCTTTAGAT AAAGAGAAAG GTGAAACACT GCTGAGTCCT 
TTGGTGATGT GTGGTCCCCA TGGCCTCAAG TTCCTGAAGC CTGTGGAGCT GCGCTTACCA 
CACTGTGCGT CCATGACTCC TGACGGTTGG TCTTTTGCTC TAAAATCATC CGACTCCTCG 
TCGGGTGATC CTAAAACCTG GCAAAACAAG TGTCTTCCCG GAGATCCAAA TTATCTCGTT 
GGAGCAAACT GTGTTTCTGT CCTTATTGAC CACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_009428688.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009426963.2 Pan troglodytes tight junction protein 1 (TJP1), transcript variant X6, mRNA.

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CloneID	OPc97831
Clone ID Related Accession (Same CDS sequence)	XM_009428688.3
Accession Version	XM_009428688.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5307bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	tight junction protein ZO-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036894.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009428688.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGGT TTGGAATTGC AATATCTGGT 
GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG 
AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA 
GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT 
CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA 
AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT 
GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG 
CCCGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT 
CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT 
GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT 
GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA 
GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT 
AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT 
GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT 
TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AGCCAGATGT GGATTTACCT 
GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC 
AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA 
AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA 
GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT 
CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC 
TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT 
TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA 
GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA 
GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG 
TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA 
ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATATGAGCG ATCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT 
TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA 
CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA 
CAGAAAGCAG AAGCTTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA 
TCAACCTCTG CTGTTAATCA TAATGTAAAT TTAACTAATG TCAGACTGGA GGAGCCCACC 
CCAGCTCCTT CCACCTCTTA CTCACCACAA GCTGATTCTT TAAGAACACC AAGTACTGAG 
GCAGCTCACA TAATGCTAAG AGATCAAGAA CCATCATTGT CGTCGCATGT AGATCCAACA 
AAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA 
CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA 
CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA 
TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC 
GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC 
GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC 
GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG 
CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA 
GCAGGGCCCA AGCCCGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG 
CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT 
GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC 
ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG 
CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG 
ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT 
TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAGTTCAG TGAACATGAC 
AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATC 
GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA 
TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC 
TCCGAGCCTG CAAAGCCAGC TCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA 
AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA 
CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG 
CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA 
GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT 
AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGCCAGC TTTCTATCCC 
CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC 
ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA 
CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA 
CCTGAATTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG 
GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT 
AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT 
GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT 
AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGCG TTAGTATAAT TATCCCTCAA 
GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC 
ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT 
GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGCGTCC 
ATGACTCCTG ACGGTTGGTC TTTTGCTCTA AAATCATCCG ACTCCTCGTC GGGTGATCCT 
AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT 
GTTTCTGTCC TTATTGACCA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009426963.2
CDS	476..5782
Translation	MSARAAAAKSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQKAEASSPVPYLSPETNPASSTSAVNHNVNLTNVRLEEPTPAPSTSYSPQADSLRTPSTEAAHIMLRDQEPSLSSHVDPTKVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQPAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPELSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_009428688.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes tight junction protein 1 (TJP1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009428688.3

ORF Insert Sequence:

ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGGT TTGGAATTGC AATATCTGGT 
GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG 
AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA 
GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT 
CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA 
AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT 
GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG 
CCCGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT 
CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT 
GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT 
GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA 
GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT 
AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT 
GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT 
TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AGCCAGATGT GGATTTACCT 
GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC 
AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA 
AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA 
GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT 
CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC 
TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT 
TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA 
GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA 
GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG 
TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA 
ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATATGAGCG ATCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT 
TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA 
CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA 
CAGAAAGCAG AAGCTTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA 
TCAACCTCTG CTGTTAATCA TAATGTAAAT TTAACTAATG TCAGACTGGA GGAGCCCACC 
CCAGCTCCTT CCACCTCTTA CTCACCACAA GCTGATTCTT TAAGAACACC AAGTACTGAG 
GCAGCTCACA TAATGCTAAG AGATCAAGAA CCATCATTGT CGTCGCATGT AGATCCAACA 
AAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA 
CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA 
CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA 
TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC 
GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC 
GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC 
GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG 
CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA 
GCAGGGCCCA AGCCCGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG 
CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT 
GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC 
ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG 
CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG 
ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT 
TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAGTTCAG TGAACATGAC 
AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATC 
GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA 
TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC 
TCCGAGCCTG CAAAGCCAGC TCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA 
AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA 
CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG 
CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA 
GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT 
AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGCCAGC TTTCTATCCC 
CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC 
ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA 
CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA 
CCTGAATTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG 
GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT 
AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT 
GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT 
AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGCG TTAGTATAAT TATCCCTCAA 
GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC 
ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT 
GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGCGTCC 
ATGACTCCTG ACGGTTGGTC TTTTGCTCTA AAATCATCCG ACTCCTCGTC GGGTGATCCT 
AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT 
GTTTCTGTCC TTATTGACCA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_024349006.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_024204774.1 Pan troglodytes tight junction protein 1 (TJP1), transcript variant X7, mRNA.

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CloneID	OPc97832
Clone ID Related Accession (Same CDS sequence)	XM_024349006.1
Accession Version	XM_024349006.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5307bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	tight junction protein ZO-1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036894.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG 
CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA 
AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC 
AGGGCTCCTG GATTTGGGTT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA 
CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA 
AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT 
GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT 
AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG 
TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CCGCTAAACC TACTAAAGTC 
ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT 
GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT 
GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA 
TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG 
CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC 
GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT 
CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG 
CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT 
AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG 
GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT 
GTGTATGCCC AAGTTGGGCA GCCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC 
CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG 
CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT 
CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT 
GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT 
ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG 
TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA 
ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA 
ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT 
ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC 
ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT 
GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT 
CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT 
CGGAAAAGTG CCAGGAAGTT ATATGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT 
CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA 
GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT 
AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG 
ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA 
ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT 
CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGGTGTATAG AAAGGATCCA 
TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA 
GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG 
AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG 
GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT 
GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT 
AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG 
TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA 
CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA 
CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCCGCAGAG 
TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT 
ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA 
CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC 
CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA 
GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC 
ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT 
CCCAAACCCA CTTCTCAGAA TCAGTTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA 
GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATCG TTCGGTCCAA TCATTATGAC 
CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT 
GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCT 
CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT 
GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT 
CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC 
CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT 
TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA 
CCAAACCGAG AAGATACTGC TCAGCCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA 
GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC 
ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA 
TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGAATTGTC TTCAAAAACT 
CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC 
AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC 
AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA 
GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG 
AGTTCCATAG AAACTGGCGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT 
GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA 
GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC 
CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGCGTCCA TGACTCCTGA CGGTGATCCT 
AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT 
GTTTCTGTCC TTATTGACCA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024204774.1
CDS	16..5322
Translation	MRRLEGLCLRRRRRRRGPARERSGDKMKYQKYLTVLQMAIGVTPSNRGSLLPLKRKLWVTPSSENPNGATSSVSQGKPSLRRIKGRLHRSKSLDSMDFCELTSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQPAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPELSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_024349006.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes tight junction protein 1 (TJP1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024349006.1

ORF Insert Sequence:

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG 
CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA 
AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC 
AGGGCTCCTG GATTTGGGTT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA 
CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA 
AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT 
GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT 
AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG 
TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CCGCTAAACC TACTAAAGTC 
ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT 
GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT 
GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA 
TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG 
CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC 
GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT 
CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG 
CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT 
AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG 
GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT 
GTGTATGCCC AAGTTGGGCA GCCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC 
CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG 
CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT 
CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT 
GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT 
ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG 
TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA 
ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA 
ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT 
ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC 
ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT 
GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT 
CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT 
CGGAAAAGTG CCAGGAAGTT ATATGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT 
CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA 
GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT 
AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG 
ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA 
ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT 
CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGGTGTATAG AAAGGATCCA 
TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA 
GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG 
AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG 
GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT 
GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT 
AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG 
TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA 
CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA 
CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCCGCAGAG 
TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT 
ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA 
CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC 
CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA 
GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC 
ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT 
CCCAAACCCA CTTCTCAGAA TCAGTTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA 
GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATCG TTCGGTCCAA TCATTATGAC 
CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT 
GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCT 
CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT 
GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT 
CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC 
CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT 
TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA 
CCAAACCGAG AAGATACTGC TCAGCCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA 
GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC 
ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA 
TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGAATTGTC TTCAAAAACT 
CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC 
AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC 
AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA 
GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG 
AGTTCCATAG AAACTGGCGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT 
GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA 
GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC 
CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGCGTCCA TGACTCCTGA CGGTGATCCT 
AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT 
GTTTCTGTCC TTATTGACCA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_009428690.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009426965.2 Pan troglodytes tight junction protein 1 (TJP1), transcript variant X8, mRNA.

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CloneID	OPc97833
Clone ID Related Accession (Same CDS sequence)	XM_009428690.3
Accession Version	XM_009428690.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5286bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	tight junction protein ZO-1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036894.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009428690.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG 
CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA 
AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC 
AGGGCTCCTG GATTTGGGTT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA 
CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA 
AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT 
GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT 
AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG 
TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CCGCTAAACC TACTAAAGTC 
ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT 
GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT 
GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA 
TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG 
CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC 
GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT 
CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG 
CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT 
AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG 
GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT 
GTGTATGCCC AAGTTGGGCA GCCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC 
CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG 
CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT 
CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT 
GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT 
ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG 
TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA 
ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA 
ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT 
ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC 
ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT 
GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT 
CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT 
CGGAAAAGTG CCAGGAAGTT ATATGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT 
CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA 
GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT 
AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG 
ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA 
ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT 
CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGGTGTATAG AAAGGATCCA 
TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA 
GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG 
AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG 
GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT 
GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT 
AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG 
TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA 
CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA 
CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCCGCAGAG 
TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT 
ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA 
CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC 
CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA 
GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC 
ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT 
CCCAAACCCA CTTCTCAGAA TCAGTTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA 
GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATCG TTCGGTCCAA TCATTATGAC 
CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT 
GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCT 
CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT 
GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT 
CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC 
CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT 
TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA 
CCAAACCGAG AAGATACTGC TCAGCCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA 
GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC 
ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA 
TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGAATTGTC TTCAAAAACT 
CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC 
AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC 
AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA 
GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG 
AGTTCCATAG AAACTGGCGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT 
GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA 
GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC 
CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGATCCTA AAACCTGGCA AAACAAGTGT 
CTTCCCGGAG ATCCAAATTA TCTCGTTGGA GCAAACTGTG TTTCTGTCCT TATTGACCAC 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009426965.2
CDS	16..5301
Translation	MRRLEGLCLRRRRRRRGPARERSGDKMKYQKYLTVLQMAIGVTPSNRGSLLPLKRKLWVTPSSENPNGATSSVSQGKPSLRRIKGRLHRSKSLDSMDFCELTSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQPAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPELSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_009428690.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes tight junction protein 1 (TJP1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009428690.3

ORF Insert Sequence:

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG 
CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA 
AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC 
AGGGCTCCTG GATTTGGGTT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA 
CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA 
AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT 
GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT 
AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG 
TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CCGCTAAACC TACTAAAGTC 
ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT 
GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT 
GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA 
TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG 
CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC 
GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT 
CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG 
CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT 
AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG 
GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT 
GTGTATGCCC AAGTTGGGCA GCCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC 
CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG 
CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT 
CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT 
GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT 
ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG 
TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA 
ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA 
ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT 
ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC 
ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT 
GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT 
CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT 
CGGAAAAGTG CCAGGAAGTT ATATGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT 
CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA 
GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT 
AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG 
ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA 
ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT 
CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGGTGTATAG AAAGGATCCA 
TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA 
GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG 
AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG 
GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT 
GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT 
AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG 
TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA 
CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA 
CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCCGCAGAG 
TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT 
ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA 
CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC 
CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA 
GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC 
ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT 
CCCAAACCCA CTTCTCAGAA TCAGTTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA 
GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATCG TTCGGTCCAA TCATTATGAC 
CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT 
GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCT 
CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT 
GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT 
CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC 
CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT 
TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA 
CCAAACCGAG AAGATACTGC TCAGCCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA 
GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC 
ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA 
TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGAATTGTC TTCAAAAACT 
CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC 
AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC 
AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA 
GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG 
AGTTCCATAG AAACTGGCGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT 
GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA 
GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC 
CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGATCCTA AAACCTGGCA AAACAAGTGT 
CTTCCCGGAG ATCCAAATTA TCTCGTTGGA GCAAACTGTG TTTCTGTCCT TATTGACCAC 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_024349007.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_024204775.1 Pan troglodytes tight junction protein 1 (TJP1), transcript variant X9, mRNA.

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CloneID	OPc97834
Clone ID Related Accession (Same CDS sequence)	XM_024349007.1
Accession Version	XM_024349007.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5280bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	tight junction protein ZO-1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036894.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGAA ATACGTTTTC AATGGATTGC CACAGTAAGA GCACAGCAAT GGAGGAAACA 
GCTATATGGG AACAACATAC AGTGACGCTT CACAGGGCTC CTGGATTTGG GTTTGGAATT 
GCAATATCTG GTGGACGAGA TAATCCTCAT TTTCAGAGTG GGGAAACGTC AATAGTGATT 
TCAGATGTGC TGAAAGGAGG ACCAGCTGAA GGACAGCTAC AGGAAAATGA CCGAGTTGCA 
ATGGTTAACG GAGTTTCAAT GGATAATGTT GAACATGCTT TTGCTGTTCA GCAACTAAGG 
AAAAGTGGGA AAAATGCAAA AATTACAATT AGAAGGAAGA AGAAAGTTCA AATACCAGTA 
AGTCGTCCTG ATCCTGAACC AGTATCTGAT AATGAAGAAG ATAGTTATGA TGAGGAAATA 
CATGATCCAA GAAGTGGCCG GAGTGGTGTG GTTAACAGAA GGAGTGAGAA GATTTGGCCG 
AGGGATAGAA GTGCAAGTAG AGAGAGGAGC TTGTCCCCGC GGTCAGACAG GCGGTCAGTG 
GCTTCCAGCC AGCCCGCTAA ACCTACTAAA GTCACACTGG TGAAATCCCG GAAAAATGAA 
GAATATGGTC TTCGATTGGC AAGCCATATA TTTGTTAAGG AAATTTCACA AGATAGTTTG 
GCAGCAAGAG ATGGCAATAT TCAAGAAGGT GATGTTGTAT TGAAGATAAA TGGTACTGTG 
ACAGAAAATA TGTCATTGAC AGATGCAAAG ACATTGATAG AAAGGTCTAA AGGCAAATTA 
AAAATGGTAG TTCAAAGAGA TGAACGGGCT ACGCTATTGA ATGTCCCTGA TCTTTCTGAC 
AGCATCCACT CTGCTAATGC CTCTGAGAGA GACGACATTT CAGAAATTCA GTCACTGGCA 
TCAGATCATT CTGGTCGATC ACACGATAGG CCTCCCCGCC GCAGCCGGTC ACGATCTCCT 
GACCAGCGGT CAGAGCCTTC TGATCATTCC AGGCACTCGC CGCAGCAGCC AAGCAATGGC 
AGTCTCCGGA GTAGAGATGA AGAGAGAATT TCTAAACCTG GGGCTGTCTC AACTCCTGTA 
AAGCATGCTG ATGATCACAC ACCTAAAACA GTGGAAGAAG TTACAGTTGA AAGAAATGAG 
AAACAAACAC CTTCTCTTCC AGAACCAAAG CCTGTGTATG CCCAAGTTGG GCAGCCAGAT 
GTGGATTTAC CTGTCAGTCC ATCTGATGGT GTCCTACCTA ATTCAACTCA TGAAGATGGG 
ATTCTTCGGC CCAGCATGAA ATTGGTAAAA TTCAGAAAAG GAGATAGTGT GGGTTTGCGG 
CTGGCTGGTG GAAATGATGT TGGAATATTT GTAGCTGGCG TTCTAGAAGA TAGCCCTGCA 
GCCAAGGAAG GCTTAGAGGA AGGTGATCAA ATTCTCAGGG TAAACAACGT AGATTTTACA 
AATATCATAA GAGAAGAAGC CGTCCTTTTC CTGCTTGACC TCCCTAAAGG AGAAGAAGTG 
ACCATATTGG CTCAGAAGAA GAAGGATGTT TATCGTCGCA TTGTAGAATC AGATGTAGGA 
GATTCTTTCT ATATTAGAAC CCATTTTGAA TATGAAAAGG AATCTCCCTA TGGACTTAGT 
TTTAACAAAG GAGAGGTGTT CCGTGTTGTG GATACCTTGT ACAATGGAAA ACTGGGCTCT 
TGGCTTGCTA TTCGAATTGG TAAAAATCAT AAGGAGGTAG AACGAGGCAT CATCCCTAAT 
AAGAACAGAG CTGAGCAGCT AGCCAGTGTA CAGTATACAC TTCCAAAAAC AGCAGGCGGA 
GACCGTGCTG ACTTCTGGAG ATTCAGAGGT CTTCGCAGCT CCAAGAGAAA TCTTCGAAAA 
AGCAGAGAGG ATTTGTCCGC TCAGCCTGTT CAAACAAAGT TTCCAGCTTA TGAAAGAGTG 
GTTCTTCGAG AAGCTGGATT TCTGAGGCCT GTAACCATTT TTGGACCAAT AGCTGATGTT 
GCCAGAGAAA AGCTGGCAAG AGAAGAACCA GATATTTATC AAATTGCAAA GAGTGAACCA 
CGAGACGCTG GAACTGACCA ACGTAGCTCT GGCATTATTC GCCTGCATAC AATAAAGCAA 
ATCATAGATC AAGACAAACA TGCTTTATTA GATGTAACAC CAAATGCAGT TGATCGTCTT 
AACTATGCCC AGTGGTATCC AATTGTTGTA TTTCTTAACC CTGATTCTAA GCAAGGAGTA 
AAAACAATGA GAATGAGGTT ATGTCCAGAA TCTCGGAAAA GTGCCAGGAA GTTATATGAG 
CGATCTCATA AACTTCGTAA AAATAATCAC CATCTTTTTA CAACTACAAT TAACTTAAAT 
TCAATGAATG ATGGTTGGTA TGGTGCGCTG AAAGAAGCAA TTCAACAACA GCAAAACCAG 
CTGGTATGGG TTTCCGAGGG AAAGGCGGAT GGTGCTACAA GTGATGACCT TGATTTGCAT 
GATGATCGTC TGTCCTACCT GTCAGCTCCA GGTAGTGAAT ACTCAATGTA TAGCACGGAC 
AGTAGACACA CTTCTGACTA TGAAGACACA GACACAGAAG GCGGGGCCTA CACTGATCAA 
GAACTAGATG AAACTCTTAA TGATGAGGTT GGGACTCCAC CGGAGTCTGC CATTACACGG 
TCCTCTGAGC CTGTAAGAGA GGACTCCTCT GGAATGCATC ATGAAAACCA AACATATCCT 
CCTTACTCAC CACAAGCGCA GCCACAACCA ATTCATAGAA TAGACTCCCC TGGATTTAAG 
CCAGCCTCTC AACAGAAAGC AGAAGCTTCA TCTCCAGTCC CTTACCTTTC GCCTGAAACA 
AACCCAGCAT CATCAACCTC TGCTGTTAAT CATAATGTAA ATTTAACTAA TGTCAGACTG 
GAGGAGCCCA CCCCAGCTCC TTCCACCTCT TACTCACCAC AAGCTGATTC TTTAAGAACA 
CCAAGTACTG AGGCAGCTCA CATAATGCTA AGAGATCAAG AACCATCATT GTCGTCGCAT 
GTAGATCCAA CAAAGGTGTA TAGAAAGGAT CCATATCCCG AGGAAATGAT GAGGCAGAAC 
CATGTTTTGA AACAGCCAGC CGTTAGTCAC CCAGGGCACA GGCCAGACAA AGAGCCTAAT 
CTGACCTATG AACCCCAACT CCCATACGTA GAGAAACAAG CCAGCAGAGA CCTCGAGCAG 
CCCACATACA GATACGAGTC CTCAAGCTAT ACGGACCAGT TTTCTCGAAA CTATGAACAT 
CGTCTGCGAT ACGAAGATCG CGTCCCCATG TATGAAGAAC AGTGGTCATA TTATGATGAC 
AAACAGCCCT ACCCATCTCG GCCACCTTTT GATAATCAGC ACTCTCAAGA CCTTGACTCC 
AGACAGCATC CCGAAGAGTC CTCAGAACGA GGGTACTTTC CACGTTTTGA AGAGCCAGCC 
CCTCTGTCTT ACGACAGCAG ACCACGTTAC GAACAGGCAC CTAGAGCATC CGCCCTGCGG 
CACGAAGAGC AGCCAGCTCC TGGGTATGAC ACACATGGTA GACTCAGACC GGAAGCCCAG 
CCCCACCCTT CAGCAGGGCC CAAGCCCGCA GAGTCCAAGC AGTATTTTGA GCAATATTCA 
CGCAGTTACG AGCAAGTACC ACCCCAAGGA TTTACCTCTA GAGCAGGTCA TTTTGAGCCT 
CTCCATGGTG CTGCAGCTGT CCCTCCGCTG ATACCTTCAT CTCAGCATAA GCCAGAAGCT 
CTGCCTTCAA ACACCAAACC ACTGCCTCCA CCCCCAACTC AAACCGAAGA AGAGGAAGAT 
CCAGCAATGA AGCCACAGTC TGTACTCACC AGAGTTAAGA TGTTTGAAAA CAAAAGATCT 
GCATCCTTAG AGACCAAGAA GGATGTAAAT GACACTGGCA GTTTTAAGCC TCCAGAAGTA 
GCATCTAAAC CTTCAGGTGC TCCCATCATT GGTCCCAAAC CCACTTCTCA GAATCAGTTC 
AGTGAACATG ACAAAACTCT GTACAGGATC CCAGAACCTC AAAAACCTCA ACTGAAGCCA 
CCTGAAGATA TCGTTCGGTC CAATCATTAT GACCCTGAAG AAGATGAAGA ATATTATCGA 
AAACAGCTGT CATACTTTGA CCGAAGAAGT TTTGAGAATA AGCCTCCTGC ACACATTGCC 
GCCAGCCATC TCTCCGAGCC TGCAAAGCCA GCTCATTCTC AGAATCAATC AAATTTTTCT 
AGTTATTCTT CAAAGGGAAA GCCTCCTGAA GCTGATGGTG TGGATAGATC ATTTGGCGAG 
AAACGCTATG AACCCATCCA GGCCACTCCC CCTCCTCCTC CATTGCCCTC GCAGTATGCC 
CAGCCATCTC AGCCTGTCAC CAGCGCGTCT CTCCACATAC ATTCTAAGGG AGCACATGGT 
GAAGGTAATT CAGTGTCATT GGATTTTCAG AATTCCTTAG TGTCCAAACC AGACCCACCT 
CCATCTCAGA ATAAGCCAGC AACTTTCAGA CCACCAAACC GAGAAGATAC TGCTCAGCCA 
GCTTTCTATC CCCAGAAAAG TTTTCCAGAT AAAGCCCCAG TTAATGGAAC TGAACAGACT 
CAGAAAACAG TCACTCCAGC ATACAATCGA TTCACACCAA AACCATATAC AAGTTCTGCC 
CGACCATTTG AACGCAAGTT TGAAAGTCCT AAATTCAATC ACAATCTTCT GCCAAGTGAA 
ACTGCACATA AACCTGAATT GTCTTCAAAA ACTCCCACTT CTCCAAAAAC TCTTGTGAAA 
TCGCACAGTT TGGCACAGCC TCCTGAGTTT GACAGTGGAG TTGAAACTTT CTCTATCCAT 
GCAGAGAAGC CTAAATATCA AATAAATAAT ATCAGCACAG TGCCTAAAGC TATTCCTGTG 
AGTCCTTCAG CTGTGGAAGA GGATGAAGAT GAAGATGGTC ATACTGTGGT GGCCACAGCC 
CGAGGCATAT TTAACAGCAA TGGGGGCGTG CTGAGTTCCA TAGAAACTGG CGTTAGTATA 
ATTATCCCTC AAGGAGCCAT TCCCGAAGGA GTTGAGCAGG AAATCTATTT CAAGGTCTGC 
CGGGACAACA GCATCCTTCC ACCTTTAGAT AAAGAGAAAG GTGAAACACT GCTGAGTCCT 
TTGGTGATGT GTGGTCCCCA TGGCCTCAAG TTCCTGAAGC CTGTGGAGCT GCGCTTACCA 
CACTGTGCGT CCATGACTCC TGACGGTGAT CCTAAAACCT GGCAAAACAA GTGTCTTCCC 
GGAGATCCAA ATTATCTCGT TGGAGCAAAC TGTGTTTCTG TCCTTATTGA CCACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024204775.1
CDS	110..5389
Translation	MERNTFSMDCHSKSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQKAEASSPVPYLSPETNPASSTSAVNHNVNLTNVRLEEPTPAPSTSYSPQADSLRTPSTEAAHIMLRDQEPSLSSHVDPTKVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQPAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPELSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_024349007.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes tight junction protein 1 (TJP1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024349007.1

ORF Insert Sequence:

ATGGAGAGAA ATACGTTTTC AATGGATTGC CACAGTAAGA GCACAGCAAT GGAGGAAACA 
GCTATATGGG AACAACATAC AGTGACGCTT CACAGGGCTC CTGGATTTGG GTTTGGAATT 
GCAATATCTG GTGGACGAGA TAATCCTCAT TTTCAGAGTG GGGAAACGTC AATAGTGATT 
TCAGATGTGC TGAAAGGAGG ACCAGCTGAA GGACAGCTAC AGGAAAATGA CCGAGTTGCA 
ATGGTTAACG GAGTTTCAAT GGATAATGTT GAACATGCTT TTGCTGTTCA GCAACTAAGG 
AAAAGTGGGA AAAATGCAAA AATTACAATT AGAAGGAAGA AGAAAGTTCA AATACCAGTA 
AGTCGTCCTG ATCCTGAACC AGTATCTGAT AATGAAGAAG ATAGTTATGA TGAGGAAATA 
CATGATCCAA GAAGTGGCCG GAGTGGTGTG GTTAACAGAA GGAGTGAGAA GATTTGGCCG 
AGGGATAGAA GTGCAAGTAG AGAGAGGAGC TTGTCCCCGC GGTCAGACAG GCGGTCAGTG 
GCTTCCAGCC AGCCCGCTAA ACCTACTAAA GTCACACTGG TGAAATCCCG GAAAAATGAA 
GAATATGGTC TTCGATTGGC AAGCCATATA TTTGTTAAGG AAATTTCACA AGATAGTTTG 
GCAGCAAGAG ATGGCAATAT TCAAGAAGGT GATGTTGTAT TGAAGATAAA TGGTACTGTG 
ACAGAAAATA TGTCATTGAC AGATGCAAAG ACATTGATAG AAAGGTCTAA AGGCAAATTA 
AAAATGGTAG TTCAAAGAGA TGAACGGGCT ACGCTATTGA ATGTCCCTGA TCTTTCTGAC 
AGCATCCACT CTGCTAATGC CTCTGAGAGA GACGACATTT CAGAAATTCA GTCACTGGCA 
TCAGATCATT CTGGTCGATC ACACGATAGG CCTCCCCGCC GCAGCCGGTC ACGATCTCCT 
GACCAGCGGT CAGAGCCTTC TGATCATTCC AGGCACTCGC CGCAGCAGCC AAGCAATGGC 
AGTCTCCGGA GTAGAGATGA AGAGAGAATT TCTAAACCTG GGGCTGTCTC AACTCCTGTA 
AAGCATGCTG ATGATCACAC ACCTAAAACA GTGGAAGAAG TTACAGTTGA AAGAAATGAG 
AAACAAACAC CTTCTCTTCC AGAACCAAAG CCTGTGTATG CCCAAGTTGG GCAGCCAGAT 
GTGGATTTAC CTGTCAGTCC ATCTGATGGT GTCCTACCTA ATTCAACTCA TGAAGATGGG 
ATTCTTCGGC CCAGCATGAA ATTGGTAAAA TTCAGAAAAG GAGATAGTGT GGGTTTGCGG 
CTGGCTGGTG GAAATGATGT TGGAATATTT GTAGCTGGCG TTCTAGAAGA TAGCCCTGCA 
GCCAAGGAAG GCTTAGAGGA AGGTGATCAA ATTCTCAGGG TAAACAACGT AGATTTTACA 
AATATCATAA GAGAAGAAGC CGTCCTTTTC CTGCTTGACC TCCCTAAAGG AGAAGAAGTG 
ACCATATTGG CTCAGAAGAA GAAGGATGTT TATCGTCGCA TTGTAGAATC AGATGTAGGA 
GATTCTTTCT ATATTAGAAC CCATTTTGAA TATGAAAAGG AATCTCCCTA TGGACTTAGT 
TTTAACAAAG GAGAGGTGTT CCGTGTTGTG GATACCTTGT ACAATGGAAA ACTGGGCTCT 
TGGCTTGCTA TTCGAATTGG TAAAAATCAT AAGGAGGTAG AACGAGGCAT CATCCCTAAT 
AAGAACAGAG CTGAGCAGCT AGCCAGTGTA CAGTATACAC TTCCAAAAAC AGCAGGCGGA 
GACCGTGCTG ACTTCTGGAG ATTCAGAGGT CTTCGCAGCT CCAAGAGAAA TCTTCGAAAA 
AGCAGAGAGG ATTTGTCCGC TCAGCCTGTT CAAACAAAGT TTCCAGCTTA TGAAAGAGTG 
GTTCTTCGAG AAGCTGGATT TCTGAGGCCT GTAACCATTT TTGGACCAAT AGCTGATGTT 
GCCAGAGAAA AGCTGGCAAG AGAAGAACCA GATATTTATC AAATTGCAAA GAGTGAACCA 
CGAGACGCTG GAACTGACCA ACGTAGCTCT GGCATTATTC GCCTGCATAC AATAAAGCAA 
ATCATAGATC AAGACAAACA TGCTTTATTA GATGTAACAC CAAATGCAGT TGATCGTCTT 
AACTATGCCC AGTGGTATCC AATTGTTGTA TTTCTTAACC CTGATTCTAA GCAAGGAGTA 
AAAACAATGA GAATGAGGTT ATGTCCAGAA TCTCGGAAAA GTGCCAGGAA GTTATATGAG 
CGATCTCATA AACTTCGTAA AAATAATCAC CATCTTTTTA CAACTACAAT TAACTTAAAT 
TCAATGAATG ATGGTTGGTA TGGTGCGCTG AAAGAAGCAA TTCAACAACA GCAAAACCAG 
CTGGTATGGG TTTCCGAGGG AAAGGCGGAT GGTGCTACAA GTGATGACCT TGATTTGCAT 
GATGATCGTC TGTCCTACCT GTCAGCTCCA GGTAGTGAAT ACTCAATGTA TAGCACGGAC 
AGTAGACACA CTTCTGACTA TGAAGACACA GACACAGAAG GCGGGGCCTA CACTGATCAA 
GAACTAGATG AAACTCTTAA TGATGAGGTT GGGACTCCAC CGGAGTCTGC CATTACACGG 
TCCTCTGAGC CTGTAAGAGA GGACTCCTCT GGAATGCATC ATGAAAACCA AACATATCCT 
CCTTACTCAC CACAAGCGCA GCCACAACCA ATTCATAGAA TAGACTCCCC TGGATTTAAG 
CCAGCCTCTC AACAGAAAGC AGAAGCTTCA TCTCCAGTCC CTTACCTTTC GCCTGAAACA 
AACCCAGCAT CATCAACCTC TGCTGTTAAT CATAATGTAA ATTTAACTAA TGTCAGACTG 
GAGGAGCCCA CCCCAGCTCC TTCCACCTCT TACTCACCAC AAGCTGATTC TTTAAGAACA 
CCAAGTACTG AGGCAGCTCA CATAATGCTA AGAGATCAAG AACCATCATT GTCGTCGCAT 
GTAGATCCAA CAAAGGTGTA TAGAAAGGAT CCATATCCCG AGGAAATGAT GAGGCAGAAC 
CATGTTTTGA AACAGCCAGC CGTTAGTCAC CCAGGGCACA GGCCAGACAA AGAGCCTAAT 
CTGACCTATG AACCCCAACT CCCATACGTA GAGAAACAAG CCAGCAGAGA CCTCGAGCAG 
CCCACATACA GATACGAGTC CTCAAGCTAT ACGGACCAGT TTTCTCGAAA CTATGAACAT 
CGTCTGCGAT ACGAAGATCG CGTCCCCATG TATGAAGAAC AGTGGTCATA TTATGATGAC 
AAACAGCCCT ACCCATCTCG GCCACCTTTT GATAATCAGC ACTCTCAAGA CCTTGACTCC 
AGACAGCATC CCGAAGAGTC CTCAGAACGA GGGTACTTTC CACGTTTTGA AGAGCCAGCC 
CCTCTGTCTT ACGACAGCAG ACCACGTTAC GAACAGGCAC CTAGAGCATC CGCCCTGCGG 
CACGAAGAGC AGCCAGCTCC TGGGTATGAC ACACATGGTA GACTCAGACC GGAAGCCCAG 
CCCCACCCTT CAGCAGGGCC CAAGCCCGCA GAGTCCAAGC AGTATTTTGA GCAATATTCA 
CGCAGTTACG AGCAAGTACC ACCCCAAGGA TTTACCTCTA GAGCAGGTCA TTTTGAGCCT 
CTCCATGGTG CTGCAGCTGT CCCTCCGCTG ATACCTTCAT CTCAGCATAA GCCAGAAGCT 
CTGCCTTCAA ACACCAAACC ACTGCCTCCA CCCCCAACTC AAACCGAAGA AGAGGAAGAT 
CCAGCAATGA AGCCACAGTC TGTACTCACC AGAGTTAAGA TGTTTGAAAA CAAAAGATCT 
GCATCCTTAG AGACCAAGAA GGATGTAAAT GACACTGGCA GTTTTAAGCC TCCAGAAGTA 
GCATCTAAAC CTTCAGGTGC TCCCATCATT GGTCCCAAAC CCACTTCTCA GAATCAGTTC 
AGTGAACATG ACAAAACTCT GTACAGGATC CCAGAACCTC AAAAACCTCA ACTGAAGCCA 
CCTGAAGATA TCGTTCGGTC CAATCATTAT GACCCTGAAG AAGATGAAGA ATATTATCGA 
AAACAGCTGT CATACTTTGA CCGAAGAAGT TTTGAGAATA AGCCTCCTGC ACACATTGCC 
GCCAGCCATC TCTCCGAGCC TGCAAAGCCA GCTCATTCTC AGAATCAATC AAATTTTTCT 
AGTTATTCTT CAAAGGGAAA GCCTCCTGAA GCTGATGGTG TGGATAGATC ATTTGGCGAG 
AAACGCTATG AACCCATCCA GGCCACTCCC CCTCCTCCTC CATTGCCCTC GCAGTATGCC 
CAGCCATCTC AGCCTGTCAC CAGCGCGTCT CTCCACATAC ATTCTAAGGG AGCACATGGT 
GAAGGTAATT CAGTGTCATT GGATTTTCAG AATTCCTTAG TGTCCAAACC AGACCCACCT 
CCATCTCAGA ATAAGCCAGC AACTTTCAGA CCACCAAACC GAGAAGATAC TGCTCAGCCA 
GCTTTCTATC CCCAGAAAAG TTTTCCAGAT AAAGCCCCAG TTAATGGAAC TGAACAGACT 
CAGAAAACAG TCACTCCAGC ATACAATCGA TTCACACCAA AACCATATAC AAGTTCTGCC 
CGACCATTTG AACGCAAGTT TGAAAGTCCT AAATTCAATC ACAATCTTCT GCCAAGTGAA 
ACTGCACATA AACCTGAATT GTCTTCAAAA ACTCCCACTT CTCCAAAAAC TCTTGTGAAA 
TCGCACAGTT TGGCACAGCC TCCTGAGTTT GACAGTGGAG TTGAAACTTT CTCTATCCAT 
GCAGAGAAGC CTAAATATCA AATAAATAAT ATCAGCACAG TGCCTAAAGC TATTCCTGTG 
AGTCCTTCAG CTGTGGAAGA GGATGAAGAT GAAGATGGTC ATACTGTGGT GGCCACAGCC 
CGAGGCATAT TTAACAGCAA TGGGGGCGTG CTGAGTTCCA TAGAAACTGG CGTTAGTATA 
ATTATCCCTC AAGGAGCCAT TCCCGAAGGA GTTGAGCAGG AAATCTATTT CAAGGTCTGC 
CGGGACAACA GCATCCTTCC ACCTTTAGAT AAAGAGAAAG GTGAAACACT GCTGAGTCCT 
TTGGTGATGT GTGGTCCCCA TGGCCTCAAG TTCCTGAAGC CTGTGGAGCT GCGCTTACCA 
CACTGTGCGT CCATGACTCC TGACGGTGAT CCTAAAACCT GGCAAAACAA GTGTCTTCCC 
GGAGATCCAA ATTATCTCGT TGGAGCAAAC TGTGTTTCTG TCCTTATTGA CCACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_024349008.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_024204776.1 Pan troglodytes tight junction protein 1 (TJP1), transcript variant X10, mRNA.

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CloneID	OPc97835
Clone ID Related Accession (Same CDS sequence)	XM_024349008.1
Accession Version	XM_024349008.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5268bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	tight junction protein ZO-1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036894.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGGT TTGGAATTGC AATATCTGGT 
GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG 
AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA 
GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT 
CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA 
AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT 
GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG 
CCCGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT 
CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT 
GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT 
GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA 
GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT 
AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT 
GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT 
TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AGCCAGATGT GGATTTACCT 
GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC 
AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA 
AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA 
GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT 
CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC 
TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT 
TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA 
GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA 
GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG 
TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA 
ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATATGAGCG ATCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT 
TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA 
CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA 
CAGAAAGCAG AAGCTTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA 
TCAACCTCTG CTGTTAATCA TAATGTAAAT TTAACTAATG TCAGACTGGA GGAGCCCACC 
CCAGCTCCTT CCACCTCTTA CTCACCACAA GCTGATTCTT TAAGAACACC AAGTACTGAG 
GCAGCTCACA TAATGCTAAG AGATCAAGAA CCATCATTGT CGTCGCATGT AGATCCAACA 
AAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA 
CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA 
CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA 
TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC 
GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC 
GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC 
GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG 
CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA 
GCAGGGCCCA AGCCCGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG 
CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT 
GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC 
ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG 
CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG 
ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT 
TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAGTTCAG TGAACATGAC 
AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATC 
GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA 
TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC 
TCCGAGCCTG CAAAGCCAGC TCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA 
AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA 
CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG 
CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA 
GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT 
AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGCCAGC TTTCTATCCC 
CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC 
ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA 
CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA 
CCTGAATTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG 
GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT 
AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT 
GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT 
AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGCG TTAGTATAAT TATCCCTCAA 
GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC 
ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT 
GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGCGTCC 
ATGACTCCTG ACGGTGATCC TAAAACCTGG CAAAACAAGT GTCTTCCCGG AGATCCAAAT 
TATCTCGTTG GAGCAAACTG TGTTTCTGTC CTTATTGACC ACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024204776.1
CDS	476..5743
Translation	MSARAAAAKSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQKAEASSPVPYLSPETNPASSTSAVNHNVNLTNVRLEEPTPAPSTSYSPQADSLRTPSTEAAHIMLRDQEPSLSSHVDPTKVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQPAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPELSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_024349008.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes tight junction protein 1 (TJP1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024349008.1

ORF Insert Sequence:

ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGGT TTGGAATTGC AATATCTGGT 
GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG 
AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA 
GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT 
CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA 
AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT 
GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG 
CCCGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT 
CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT 
GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT 
GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA 
GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT 
AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT 
GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT 
TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AGCCAGATGT GGATTTACCT 
GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC 
AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA 
AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA 
GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT 
CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC 
TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT 
TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA 
GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA 
GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG 
TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA 
ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATATGAGCG ATCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT 
TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA 
CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA 
CAGAAAGCAG AAGCTTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA 
TCAACCTCTG CTGTTAATCA TAATGTAAAT TTAACTAATG TCAGACTGGA GGAGCCCACC 
CCAGCTCCTT CCACCTCTTA CTCACCACAA GCTGATTCTT TAAGAACACC AAGTACTGAG 
GCAGCTCACA TAATGCTAAG AGATCAAGAA CCATCATTGT CGTCGCATGT AGATCCAACA 
AAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA 
CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA 
CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA 
TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC 
GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC 
GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC 
GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG 
CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA 
GCAGGGCCCA AGCCCGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG 
CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT 
GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC 
ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG 
CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG 
ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT 
TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAGTTCAG TGAACATGAC 
AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATC 
GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA 
TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC 
TCCGAGCCTG CAAAGCCAGC TCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA 
AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA 
CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG 
CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA 
GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT 
AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGCCAGC TTTCTATCCC 
CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC 
ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA 
CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA 
CCTGAATTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG 
GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT 
AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT 
GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT 
AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGCG TTAGTATAAT TATCCCTCAA 
GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC 
ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT 
GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGCGTCC 
ATGACTCCTG ACGGTGATCC TAAAACCTGG CAAAACAAGT GTCTTCCCGG AGATCCAAAT 
TATCTCGTTG GAGCAAACTG TGTTTCTGTC CTTATTGACC ACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_001163120.4 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_001163120.2 Pan troglodytes tight junction protein 1 (TJP1), transcript variant X11, mRNA.

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CloneID	OPc97836
Clone ID Related Accession (Same CDS sequence)	XM_001163120.4
Accession Version	XM_001163120.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5247bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	tight junction protein ZO-1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036894.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_001163120.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGGT TTGGAATTGC AATATCTGGT 
GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG 
AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA 
GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT 
CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA 
AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT 
GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG 
CCCGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT 
CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT 
GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT 
GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA 
GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT 
AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT 
GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT 
TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AGCCAGATGT GGATTTACCT 
GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC 
AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA 
AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA 
GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT 
CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC 
TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT 
TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA 
GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA 
GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG 
TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA 
ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATATGAGCG ATCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT 
TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA 
CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA 
CAGAAAGCAG AAGCTTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA 
TCAACCTCTG CTGTTAATCA TAATGTAAAT TTAACTAATG TCAGACTGGA GGAGCCCACC 
CCAGCTCCTT CCACCTCTTA CTCACCACAA GCTGATTCTT TAAGAACACC AAGTACTGAG 
GCAGCTCACA TAATGCTAAG AGATCAAGAA CCATCATTGT CGTCGCATGT AGATCCAACA 
AAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA 
CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA 
CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA 
TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC 
GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC 
GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC 
GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG 
CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA 
GCAGGGCCCA AGCCCGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG 
CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT 
GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC 
ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG 
CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG 
ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT 
TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAGTTCAG TGAACATGAC 
AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATC 
GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA 
TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC 
TCCGAGCCTG CAAAGCCAGC TCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA 
AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA 
CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG 
CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA 
GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT 
AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGCCAGC TTTCTATCCC 
CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC 
ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA 
CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA 
CCTGAATTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG 
GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT 
AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT 
GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT 
AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGCG TTAGTATAAT TATCCCTCAA 
GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC 
ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT 
GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGATCCT 
AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT 
GTTTCTGTCC TTATTGACCA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001163120.2
CDS	476..5722
Translation	MSARAAAAKSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQKAEASSPVPYLSPETNPASSTSAVNHNVNLTNVRLEEPTPAPSTSYSPQADSLRTPSTEAAHIMLRDQEPSLSSHVDPTKVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQPAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPELSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_001163120.4
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes tight junction protein 1 (TJP1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001163120.4

ORF Insert Sequence:

ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGGT TTGGAATTGC AATATCTGGT 
GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG 
AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA 
GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT 
CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA 
AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT 
GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG 
CCCGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT 
CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT 
GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT 
GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA 
GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT 
AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT 
GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT 
TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AGCCAGATGT GGATTTACCT 
GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC 
AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA 
AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA 
GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT 
CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC 
TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT 
TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA 
GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA 
GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG 
TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA 
ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATATGAGCG ATCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT 
TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA 
CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA 
CAGAAAGCAG AAGCTTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA 
TCAACCTCTG CTGTTAATCA TAATGTAAAT TTAACTAATG TCAGACTGGA GGAGCCCACC 
CCAGCTCCTT CCACCTCTTA CTCACCACAA GCTGATTCTT TAAGAACACC AAGTACTGAG 
GCAGCTCACA TAATGCTAAG AGATCAAGAA CCATCATTGT CGTCGCATGT AGATCCAACA 
AAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA 
CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA 
CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA 
TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC 
GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC 
GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC 
GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG 
CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA 
GCAGGGCCCA AGCCCGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG 
CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT 
GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC 
ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG 
CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG 
ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT 
TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAGTTCAG TGAACATGAC 
AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATC 
GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA 
TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC 
TCCGAGCCTG CAAAGCCAGC TCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA 
AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA 
CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG 
CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA 
GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT 
AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGCCAGC TTTCTATCCC 
CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC 
ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA 
CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA 
CCTGAATTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG 
GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT 
AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT 
GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT 
AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGCG TTAGTATAAT TATCCCTCAA 
GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC 
ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT 
GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGATCCT 
AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT 
GTTTCTGTCC TTATTGACCA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_016927841.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016783330.1 Pan troglodytes tight junction protein 1 (TJP1), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OPc97837
Clone ID Related Accession (Same CDS sequence)	XM_016927841.2
Accession Version	XM_016927841.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5301bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	tight junction protein ZO-1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036894.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016927841.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTCT GTGAGCTCAC TAGCACAGCA ATGGAGGAAA CAGCTATATG GGAACAACAT 
ACAGTGACGC TTCACAGGGC TCCTGGATTT GGGTTTGGAA TTGCAATATC TGGTGGACGA 
GATAATCCTC ATTTTCAGAG TGGGGAAACG TCAATAGTGA TTTCAGATGT GCTGAAAGGA 
GGACCAGCTG AAGGACAGCT ACAGGAAAAT GACCGAGTTG CAATGGTTAA CGGAGTTTCA 
ATGGATAATG TTGAACATGC TTTTGCTGTT CAGCAACTAA GGAAAAGTGG GAAAAATGCA 
AAAATTACAA TTAGAAGGAA GAAGAAAGTT CAAATACCAG TAAGTCGTCC TGATCCTGAA 
CCAGTATCTG ATAATGAAGA AGATAGTTAT GATGAGGAAA TACATGATCC AAGAAGTGGC 
CGGAGTGGTG TGGTTAACAG AAGGAGTGAG AAGATTTGGC CGAGGGATAG AAGTGCAAGT 
AGAGAGAGGA GCTTGTCCCC GCGGTCAGAC AGGCGGTCAG TGGCTTCCAG CCAGCCCGCT 
AAACCTACTA AAGTCACACT GGTGAAATCC CGGAAAAATG AAGAATATGG TCTTCGATTG 
GCAAGCCATA TATTTGTTAA GGAAATTTCA CAAGATAGTT TGGCAGCAAG AGATGGCAAT 
ATTCAAGAAG GTGATGTTGT ATTGAAGATA AATGGTACTG TGACAGAAAA TATGTCATTG 
ACAGATGCAA AGACATTGAT AGAAAGGTCT AAAGGCAAAT TAAAAATGGT AGTTCAAAGA 
GATGAACGGG CTACGCTATT GAATGTCCCT GATCTTTCTG ACAGCATCCA CTCTGCTAAT 
GCCTCTGAGA GAGACGACAT TTCAGAAATT CAGTCACTGG CATCAGATCA TTCTGGTCGA 
TCACACGATA GGCCTCCCCG CCGCAGCCGG TCACGATCTC CTGACCAGCG GTCAGAGCCT 
TCTGATCATT CCAGGCACTC GCCGCAGCAG CCAAGCAATG GCAGTCTCCG GAGTAGAGAT 
GAAGAGAGAA TTTCTAAACC TGGGGCTGTC TCAACTCCTG TAAAGCATGC TGATGATCAC 
ACACCTAAAA CAGTGGAAGA AGTTACAGTT GAAAGAAATG AGAAACAAAC ACCTTCTCTT 
CCAGAACCAA AGCCTGTGTA TGCCCAAGTT GGGCAGCCAG ATGTGGATTT ACCTGTCAGT 
CCATCTGATG GTGTCCTACC TAATTCAACT CATGAAGATG GGATTCTTCG GCCCAGCATG 
AAATTGGTAA AATTCAGAAA AGGAGATAGT GTGGGTTTGC GGCTGGCTGG TGGAAATGAT 
GTTGGAATAT TTGTAGCTGG CGTTCTAGAA GATAGCCCTG CAGCCAAGGA AGGCTTAGAG 
GAAGGTGATC AAATTCTCAG GGTAAACAAC GTAGATTTTA CAAATATCAT AAGAGAAGAA 
GCCGTCCTTT TCCTGCTTGA CCTCCCTAAA GGAGAAGAAG TGACCATATT GGCTCAGAAG 
AAGAAGGATG TTTATCGTCG CATTGTAGAA TCAGATGTAG GAGATTCTTT CTATATTAGA 
ACCCATTTTG AATATGAAAA GGAATCTCCC TATGGACTTA GTTTTAACAA AGGAGAGGTG 
TTCCGTGTTG TGGATACCTT GTACAATGGA AAACTGGGCT CTTGGCTTGC TATTCGAATT 
GGTAAAAATC ATAAGGAGGT AGAACGAGGC ATCATCCCTA ATAAGAACAG AGCTGAGCAG 
CTAGCCAGTG TACAGTATAC ACTTCCAAAA ACAGCAGGCG GAGACCGTGC TGACTTCTGG 
AGATTCAGAG GTCTTCGCAG CTCCAAGAGA AATCTTCGAA AAAGCAGAGA GGATTTGTCC 
GCTCAGCCTG TTCAAACAAA GTTTCCAGCT TATGAAAGAG TGGTTCTTCG AGAAGCTGGA 
TTTCTGAGGC CTGTAACCAT TTTTGGACCA ATAGCTGATG TTGCCAGAGA AAAGCTGGCA 
AGAGAAGAAC CAGATATTTA TCAAATTGCA AAGAGTGAAC CACGAGACGC TGGAACTGAC 
CAACGTAGCT CTGGCATTAT TCGCCTGCAT ACAATAAAGC AAATCATAGA TCAAGACAAA 
CATGCTTTAT TAGATGTAAC ACCAAATGCA GTTGATCGTC TTAACTATGC CCAGTGGTAT 
CCAATTGTTG TATTTCTTAA CCCTGATTCT AAGCAAGGAG TAAAAACAAT GAGAATGAGG 
TTATGTCCAG AATCTCGGAA AAGTGCCAGG AAGTTATATG AGCGATCTCA TAAACTTCGT 
AAAAATAATC ACCATCTTTT TACAACTACA ATTAACTTAA ATTCAATGAA TGATGGTTGG 
TATGGTGCGC TGAAAGAAGC AATTCAACAA CAGCAAAACC AGCTGGTATG GGTTTCCGAG 
GGAAAGGCGG ATGGTGCTAC AAGTGATGAC CTTGATTTGC ATGATGATCG TCTGTCCTAC 
CTGTCAGCTC CAGGTAGTGA ATACTCAATG TATAGCACGG ACAGTAGACA CACTTCTGAC 
TATGAAGACA CAGACACAGA AGGCGGGGCC TACACTGATC AAGAACTAGA TGAAACTCTT 
AATGATGAGG TTGGGACTCC ACCGGAGTCT GCCATTACAC GGTCCTCTGA GCCTGTAAGA 
GAGGACTCCT CTGGAATGCA TCATGAAAAC CAAACATATC CTCCTTACTC ACCACAAGCG 
CAGCCACAAC CAATTCATAG AATAGACTCC CCTGGATTTA AGCCAGCCTC TCAACAGAAA 
GCAGAAGCTT CATCTCCAGT CCCTTACCTT TCGCCTGAAA CAAACCCAGC ATCATCAACC 
TCTGCTGTTA ATCATAATGT AAATTTAACT AATGTCAGAC TGGAGGAGCC CACCCCAGCT 
CCTTCCACCT CTTACTCACC ACAAGCTGAT TCTTTAAGAA CACCAAGTAC TGAGGCAGCT 
CACATAATGC TAAGAGATCA AGAACCATCA TTGTCGTCGC ATGTAGATCC AACAAAGGTG 
TATAGAAAGG ATCCATATCC CGAGGAAATG ATGAGGCAGA ACCATGTTTT GAAACAGCCA 
GCCGTTAGTC ACCCAGGGCA CAGGCCAGAC AAAGAGCCTA ATCTGACCTA TGAACCCCAA 
CTCCCATACG TAGAGAAACA AGCCAGCAGA GACCTCGAGC AGCCCACATA CAGATACGAG 
TCCTCAAGCT ATACGGACCA GTTTTCTCGA AACTATGAAC ATCGTCTGCG ATACGAAGAT 
CGCGTCCCCA TGTATGAAGA ACAGTGGTCA TATTATGATG ACAAACAGCC CTACCCATCT 
CGGCCACCTT TTGATAATCA GCACTCTCAA GACCTTGACT CCAGACAGCA TCCCGAAGAG 
TCCTCAGAAC GAGGGTACTT TCCACGTTTT GAAGAGCCAG CCCCTCTGTC TTACGACAGC 
AGACCACGTT ACGAACAGGC ACCTAGAGCA TCCGCCCTGC GGCACGAAGA GCAGCCAGCT 
CCTGGGTATG ACACACATGG TAGACTCAGA CCGGAAGCCC AGCCCCACCC TTCAGCAGGG 
CCCAAGCCCG CAGAGTCCAA GCAGTATTTT GAGCAATATT CACGCAGTTA CGAGCAAGTA 
CCACCCCAAG GATTTACCTC TAGAGCAGGT CATTTTGAGC CTCTCCATGG TGCTGCAGCT 
GTCCCTCCGC TGATACCTTC ATCTCAGCAT AAGCCAGAAG CTCTGCCTTC AAACACCAAA 
CCACTGCCTC CACCCCCAAC TCAAACCGAA GAAGAGGAAG ATCCAGCAAT GAAGCCACAG 
TCTGTACTCA CCAGAGTTAA GATGTTTGAA AACAAAAGAT CTGCATCCTT AGAGACCAAG 
AAGGATGTAA ATGACACTGG CAGTTTTAAG CCTCCAGAAG TAGCATCTAA ACCTTCAGGT 
GCTCCCATCA TTGGTCCCAA ACCCACTTCT CAGAATCAGT TCAGTGAACA TGACAAAACT 
CTGTACAGGA TCCCAGAACC TCAAAAACCT CAACTGAAGC CACCTGAAGA TATCGTTCGG 
TCCAATCATT ATGACCCTGA AGAAGATGAA GAATATTATC GAAAACAGCT GTCATACTTT 
GACCGAAGAA GTTTTGAGAA TAAGCCTCCT GCACACATTG CCGCCAGCCA TCTCTCCGAG 
CCTGCAAAGC CAGCTCATTC TCAGAATCAA TCAAATTTTT CTAGTTATTC TTCAAAGGGA 
AAGCCTCCTG AAGCTGATGG TGTGGATAGA TCATTTGGCG AGAAACGCTA TGAACCCATC 
CAGGCCACTC CCCCTCCTCC TCCATTGCCC TCGCAGTATG CCCAGCCATC TCAGCCTGTC 
ACCAGCGCGT CTCTCCACAT ACATTCTAAG GGAGCACATG GTGAAGGTAA TTCAGTGTCA 
TTGGATTTTC AGAATTCCTT AGTGTCCAAA CCAGACCCAC CTCCATCTCA GAATAAGCCA 
GCAACTTTCA GACCACCAAA CCGAGAAGAT ACTGCTCAGC CAGCTTTCTA TCCCCAGAAA 
AGTTTTCCAG ATAAAGCCCC AGTTAATGGA ACTGAACAGA CTCAGAAAAC AGTCACTCCA 
GCATACAATC GATTCACACC AAAACCATAT ACAAGTTCTG CCCGACCATT TGAACGCAAG 
TTTGAAAGTC CTAAATTCAA TCACAATCTT CTGCCAAGTG AAACTGCACA TAAACCTGAA 
TTGTCTTCAA AAACTCCCAC TTCTCCAAAA ACTCTTGTGA AATCGCACAG TTTGGCACAG 
CCTCCTGAGT TTGACAGTGG AGTTGAAACT TTCTCTATCC ATGCAGAGAA GCCTAAATAT 
CAAATAAATA ATATCAGCAC AGTGCCTAAA GCTATTCCTG TGAGTCCTTC AGCTGTGGAA 
GAGGATGAAG ATGAAGATGG TCATACTGTG GTGGCCACAG CCCGAGGCAT ATTTAACAGC 
AATGGGGGCG TGCTGAGTTC CATAGAAACT GGCGTTAGTA TAATTATCCC TCAAGGAGCC 
ATTCCCGAAG GAGTTGAGCA GGAAATCTAT TTCAAGGTCT GCCGGGACAA CAGCATCCTT 
CCACCTTTAG ATAAAGAGAA AGGTGAAACA CTGCTGAGTC CTTTGGTGAT GTGTGGTCCC 
CATGGCCTCA AGTTCCTGAA GCCTGTGGAG CTGCGCTTAC CACACTGTGC GTCCATGACT 
CCTGACGGTT GGTCTTTTGC TCTAAAATCA TCCGACTCCT CGTCGGGTGA TCCTAAAACC 
TGGCAAAACA AGTGTCTTCC CGGAGATCCA AATTATCTCG TTGGAGCAAA CTGTGTTTCT 
GTCCTTATTG ACCACTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016783330.1
CDS	419..5719
Translation	MDFCELTSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQKAEASSPVPYLSPETNPASSTSAVNHNVNLTNVRLEEPTPAPSTSYSPQADSLRTPSTEAAHIMLRDQEPSLSSHVDPTKVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQPAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPELSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_016927841.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes tight junction protein 1 (TJP1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016927841.2

ORF Insert Sequence:

ATGGATTTCT GTGAGCTCAC TAGCACAGCA ATGGAGGAAA CAGCTATATG GGAACAACAT 
ACAGTGACGC TTCACAGGGC TCCTGGATTT GGGTTTGGAA TTGCAATATC TGGTGGACGA 
GATAATCCTC ATTTTCAGAG TGGGGAAACG TCAATAGTGA TTTCAGATGT GCTGAAAGGA 
GGACCAGCTG AAGGACAGCT ACAGGAAAAT GACCGAGTTG CAATGGTTAA CGGAGTTTCA 
ATGGATAATG TTGAACATGC TTTTGCTGTT CAGCAACTAA GGAAAAGTGG GAAAAATGCA 
AAAATTACAA TTAGAAGGAA GAAGAAAGTT CAAATACCAG TAAGTCGTCC TGATCCTGAA 
CCAGTATCTG ATAATGAAGA AGATAGTTAT GATGAGGAAA TACATGATCC AAGAAGTGGC 
CGGAGTGGTG TGGTTAACAG AAGGAGTGAG AAGATTTGGC CGAGGGATAG AAGTGCAAGT 
AGAGAGAGGA GCTTGTCCCC GCGGTCAGAC AGGCGGTCAG TGGCTTCCAG CCAGCCCGCT 
AAACCTACTA AAGTCACACT GGTGAAATCC CGGAAAAATG AAGAATATGG TCTTCGATTG 
GCAAGCCATA TATTTGTTAA GGAAATTTCA CAAGATAGTT TGGCAGCAAG AGATGGCAAT 
ATTCAAGAAG GTGATGTTGT ATTGAAGATA AATGGTACTG TGACAGAAAA TATGTCATTG 
ACAGATGCAA AGACATTGAT AGAAAGGTCT AAAGGCAAAT TAAAAATGGT AGTTCAAAGA 
GATGAACGGG CTACGCTATT GAATGTCCCT GATCTTTCTG ACAGCATCCA CTCTGCTAAT 
GCCTCTGAGA GAGACGACAT TTCAGAAATT CAGTCACTGG CATCAGATCA TTCTGGTCGA 
TCACACGATA GGCCTCCCCG CCGCAGCCGG TCACGATCTC CTGACCAGCG GTCAGAGCCT 
TCTGATCATT CCAGGCACTC GCCGCAGCAG CCAAGCAATG GCAGTCTCCG GAGTAGAGAT 
GAAGAGAGAA TTTCTAAACC TGGGGCTGTC TCAACTCCTG TAAAGCATGC TGATGATCAC 
ACACCTAAAA CAGTGGAAGA AGTTACAGTT GAAAGAAATG AGAAACAAAC ACCTTCTCTT 
CCAGAACCAA AGCCTGTGTA TGCCCAAGTT GGGCAGCCAG ATGTGGATTT ACCTGTCAGT 
CCATCTGATG GTGTCCTACC TAATTCAACT CATGAAGATG GGATTCTTCG GCCCAGCATG 
AAATTGGTAA AATTCAGAAA AGGAGATAGT GTGGGTTTGC GGCTGGCTGG TGGAAATGAT 
GTTGGAATAT TTGTAGCTGG CGTTCTAGAA GATAGCCCTG CAGCCAAGGA AGGCTTAGAG 
GAAGGTGATC AAATTCTCAG GGTAAACAAC GTAGATTTTA CAAATATCAT AAGAGAAGAA 
GCCGTCCTTT TCCTGCTTGA CCTCCCTAAA GGAGAAGAAG TGACCATATT GGCTCAGAAG 
AAGAAGGATG TTTATCGTCG CATTGTAGAA TCAGATGTAG GAGATTCTTT CTATATTAGA 
ACCCATTTTG AATATGAAAA GGAATCTCCC TATGGACTTA GTTTTAACAA AGGAGAGGTG 
TTCCGTGTTG TGGATACCTT GTACAATGGA AAACTGGGCT CTTGGCTTGC TATTCGAATT 
GGTAAAAATC ATAAGGAGGT AGAACGAGGC ATCATCCCTA ATAAGAACAG AGCTGAGCAG 
CTAGCCAGTG TACAGTATAC ACTTCCAAAA ACAGCAGGCG GAGACCGTGC TGACTTCTGG 
AGATTCAGAG GTCTTCGCAG CTCCAAGAGA AATCTTCGAA AAAGCAGAGA GGATTTGTCC 
GCTCAGCCTG TTCAAACAAA GTTTCCAGCT TATGAAAGAG TGGTTCTTCG AGAAGCTGGA 
TTTCTGAGGC CTGTAACCAT TTTTGGACCA ATAGCTGATG TTGCCAGAGA AAAGCTGGCA 
AGAGAAGAAC CAGATATTTA TCAAATTGCA AAGAGTGAAC CACGAGACGC TGGAACTGAC 
CAACGTAGCT CTGGCATTAT TCGCCTGCAT ACAATAAAGC AAATCATAGA TCAAGACAAA 
CATGCTTTAT TAGATGTAAC ACCAAATGCA GTTGATCGTC TTAACTATGC CCAGTGGTAT 
CCAATTGTTG TATTTCTTAA CCCTGATTCT AAGCAAGGAG TAAAAACAAT GAGAATGAGG 
TTATGTCCAG AATCTCGGAA AAGTGCCAGG AAGTTATATG AGCGATCTCA TAAACTTCGT 
AAAAATAATC ACCATCTTTT TACAACTACA ATTAACTTAA ATTCAATGAA TGATGGTTGG 
TATGGTGCGC TGAAAGAAGC AATTCAACAA CAGCAAAACC AGCTGGTATG GGTTTCCGAG 
GGAAAGGCGG ATGGTGCTAC AAGTGATGAC CTTGATTTGC ATGATGATCG TCTGTCCTAC 
CTGTCAGCTC CAGGTAGTGA ATACTCAATG TATAGCACGG ACAGTAGACA CACTTCTGAC 
TATGAAGACA CAGACACAGA AGGCGGGGCC TACACTGATC AAGAACTAGA TGAAACTCTT 
AATGATGAGG TTGGGACTCC ACCGGAGTCT GCCATTACAC GGTCCTCTGA GCCTGTAAGA 
GAGGACTCCT CTGGAATGCA TCATGAAAAC CAAACATATC CTCCTTACTC ACCACAAGCG 
CAGCCACAAC CAATTCATAG AATAGACTCC CCTGGATTTA AGCCAGCCTC TCAACAGAAA 
GCAGAAGCTT CATCTCCAGT CCCTTACCTT TCGCCTGAAA CAAACCCAGC ATCATCAACC 
TCTGCTGTTA ATCATAATGT AAATTTAACT AATGTCAGAC TGGAGGAGCC CACCCCAGCT 
CCTTCCACCT CTTACTCACC ACAAGCTGAT TCTTTAAGAA CACCAAGTAC TGAGGCAGCT 
CACATAATGC TAAGAGATCA AGAACCATCA TTGTCGTCGC ATGTAGATCC AACAAAGGTG 
TATAGAAAGG ATCCATATCC CGAGGAAATG ATGAGGCAGA ACCATGTTTT GAAACAGCCA 
GCCGTTAGTC ACCCAGGGCA CAGGCCAGAC AAAGAGCCTA ATCTGACCTA TGAACCCCAA 
CTCCCATACG TAGAGAAACA AGCCAGCAGA GACCTCGAGC AGCCCACATA CAGATACGAG 
TCCTCAAGCT ATACGGACCA GTTTTCTCGA AACTATGAAC ATCGTCTGCG ATACGAAGAT 
CGCGTCCCCA TGTATGAAGA ACAGTGGTCA TATTATGATG ACAAACAGCC CTACCCATCT 
CGGCCACCTT TTGATAATCA GCACTCTCAA GACCTTGACT CCAGACAGCA TCCCGAAGAG 
TCCTCAGAAC GAGGGTACTT TCCACGTTTT GAAGAGCCAG CCCCTCTGTC TTACGACAGC 
AGACCACGTT ACGAACAGGC ACCTAGAGCA TCCGCCCTGC GGCACGAAGA GCAGCCAGCT 
CCTGGGTATG ACACACATGG TAGACTCAGA CCGGAAGCCC AGCCCCACCC TTCAGCAGGG 
CCCAAGCCCG CAGAGTCCAA GCAGTATTTT GAGCAATATT CACGCAGTTA CGAGCAAGTA 
CCACCCCAAG GATTTACCTC TAGAGCAGGT CATTTTGAGC CTCTCCATGG TGCTGCAGCT 
GTCCCTCCGC TGATACCTTC ATCTCAGCAT AAGCCAGAAG CTCTGCCTTC AAACACCAAA 
CCACTGCCTC CACCCCCAAC TCAAACCGAA GAAGAGGAAG ATCCAGCAAT GAAGCCACAG 
TCTGTACTCA CCAGAGTTAA GATGTTTGAA AACAAAAGAT CTGCATCCTT AGAGACCAAG 
AAGGATGTAA ATGACACTGG CAGTTTTAAG CCTCCAGAAG TAGCATCTAA ACCTTCAGGT 
GCTCCCATCA TTGGTCCCAA ACCCACTTCT CAGAATCAGT TCAGTGAACA TGACAAAACT 
CTGTACAGGA TCCCAGAACC TCAAAAACCT CAACTGAAGC CACCTGAAGA TATCGTTCGG 
TCCAATCATT ATGACCCTGA AGAAGATGAA GAATATTATC GAAAACAGCT GTCATACTTT 
GACCGAAGAA GTTTTGAGAA TAAGCCTCCT GCACACATTG CCGCCAGCCA TCTCTCCGAG 
CCTGCAAAGC CAGCTCATTC TCAGAATCAA TCAAATTTTT CTAGTTATTC TTCAAAGGGA 
AAGCCTCCTG AAGCTGATGG TGTGGATAGA TCATTTGGCG AGAAACGCTA TGAACCCATC 
CAGGCCACTC CCCCTCCTCC TCCATTGCCC TCGCAGTATG CCCAGCCATC TCAGCCTGTC 
ACCAGCGCGT CTCTCCACAT ACATTCTAAG GGAGCACATG GTGAAGGTAA TTCAGTGTCA 
TTGGATTTTC AGAATTCCTT AGTGTCCAAA CCAGACCCAC CTCCATCTCA GAATAAGCCA 
GCAACTTTCA GACCACCAAA CCGAGAAGAT ACTGCTCAGC CAGCTTTCTA TCCCCAGAAA 
AGTTTTCCAG ATAAAGCCCC AGTTAATGGA ACTGAACAGA CTCAGAAAAC AGTCACTCCA 
GCATACAATC GATTCACACC AAAACCATAT ACAAGTTCTG CCCGACCATT TGAACGCAAG 
TTTGAAAGTC CTAAATTCAA TCACAATCTT CTGCCAAGTG AAACTGCACA TAAACCTGAA 
TTGTCTTCAA AAACTCCCAC TTCTCCAAAA ACTCTTGTGA AATCGCACAG TTTGGCACAG 
CCTCCTGAGT TTGACAGTGG AGTTGAAACT TTCTCTATCC ATGCAGAGAA GCCTAAATAT 
CAAATAAATA ATATCAGCAC AGTGCCTAAA GCTATTCCTG TGAGTCCTTC AGCTGTGGAA 
GAGGATGAAG ATGAAGATGG TCATACTGTG GTGGCCACAG CCCGAGGCAT ATTTAACAGC 
AATGGGGGCG TGCTGAGTTC CATAGAAACT GGCGTTAGTA TAATTATCCC TCAAGGAGCC 
ATTCCCGAAG GAGTTGAGCA GGAAATCTAT TTCAAGGTCT GCCGGGACAA CAGCATCCTT 
CCACCTTTAG ATAAAGAGAA AGGTGAAACA CTGCTGAGTC CTTTGGTGAT GTGTGGTCCC 
CATGGCCTCA AGTTCCTGAA GCCTGTGGAG CTGCGCTTAC CACACTGTGC GTCCATGACT 
CCTGACGGTT GGTCTTTTGC TCTAAAATCA TCCGACTCCT CGTCGGGTGA TCCTAAAACC 
TGGCAAAACA AGTGTCTTCC CGGAGATCCA AATTATCTCG TTGGAGCAAA CTGTGTTTCT 
GTCCTTATTG ACCACTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_016927842.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016783331.1 Pan troglodytes tight junction protein 1 (TJP1), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OPc97838
Clone ID Related Accession (Same CDS sequence)	XM_016927842.2
Accession Version	XM_016927842.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5079bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	tight junction protein ZO-1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036894.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016927842.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGAA ATACGTTTTC AATGGATTGC CACAGTAAGA GCACAGCAAT GGAGGAAACA 
GCTATATGGG AACAACATAC AGTGACGCTT CACAGGGCTC CTGGATTTGG GTTTGGAATT 
GCAATATCTG GTGGACGAGA TAATCCTCAT TTTCAGAGTG GGGAAACGTC AATAGTGATT 
TCAGATGTGC TGAAAGGAGG ACCAGCTGAA GGACAGCTAC AGGAAAATGA CCGAGTTGCA 
ATGGTTAACG GAGTTTCAAT GGATAATGTT GAACATGCTT TTGCTGTTCA GCAACTAAGG 
AAAAGTGGGA AAAATGCAAA AATTACAATT AGAAGGAAGA AGAAAGTTCA AATACCAGTA 
AGTCGTCCTG ATCCTGAACC AGTATCTGAT AATGAAGAAG ATAGTTATGA TGAGGAAATA 
CATGATCCAA GAAGTGGCCG GAGTGGTGTG GTTAACAGAA GGAGTGAGAA GATTTGGCCG 
AGGGATAGAA GTGCAAGTAG AGAGAGGAGC TTGTCCCCGC GGTCAGACAG GCGGTCAGTG 
GCTTCCAGCC AGCCCGCTAA ACCTACTAAA GTCACACTGG TGAAATCCCG GAAAAATGAA 
GAATATGGTC TTCGATTGGC AAGCCATATA TTTGTTAAGG AAATTTCACA AGATAGTTTG 
GCAGCAAGAG ATGGCAATAT TCAAGAAGGT GATGTTGTAT TGAAGATAAA TGGTACTGTG 
ACAGAAAATA TGTCATTGAC AGATGCAAAG ACATTGATAG AAAGGTCTAA AGGCAAATTA 
AAAATGGTAG TTCAAAGAGA TGAACGGGCT ACGCTATTGA ATGTCCCTGA TCTTTCTGAC 
AGCATCCACT CTGCTAATGC CTCTGAGAGA GACGACATTT CAGAAATTCA GTCACTGGCA 
TCAGATCATT CTGGTCGATC ACACGATAGG CCTCCCCGCC GCAGCCGGTC ACGATCTCCT 
GACCAGCGGT CAGAGCCTTC TGATCATTCC AGGCACTCGC CGCAGCAGCC AAGCAATGGC 
AGTCTCCGGA GTAGAGATGA AGAGAGAATT TCTAAACCTG GGGCTGTCTC AACTCCTGTA 
AAGCATGCTG ATGATCACAC ACCTAAAACA GTGGAAGAAG TTACAGTTGA AAGAAATGAG 
AAACAAACAC CTTCTCTTCC AGAACCAAAG CCTGTGTATG CCCAAGTTGG GCAGCCAGAT 
GTGGATTTAC CTGTCAGTCC ATCTGATGGT GTCCTACCTA ATTCAACTCA TGAAGATGGG 
ATTCTTCGGC CCAGCATGAA ATTGGTAAAA TTCAGAAAAG GAGATAGTGT GGGTTTGCGG 
CTGGCTGGTG GAAATGATGT TGGAATATTT GTAGCTGGCG TTCTAGAAGA TAGCCCTGCA 
GCCAAGGAAG GCTTAGAGGA AGGTGATCAA ATTCTCAGGG TAAACAACGT AGATTTTACA 
AATATCATAA GAGAAGAAGC CGTCCTTTTC CTGCTTGACC TCCCTAAAGG AGAAGAAGTG 
ACCATATTGG CTCAGAAGAA GAAGGATGTT TATCGTCGCA TTGTAGAATC AGATGTAGGA 
GATTCTTTCT ATATTAGAAC CCATTTTGAA TATGAAAAGG AATCTCCCTA TGGACTTAGT 
TTTAACAAAG GAGAGGTGTT CCGTGTTGTG GATACCTTGT ACAATGGAAA ACTGGGCTCT 
TGGCTTGCTA TTCGAATTGG TAAAAATCAT AAGGAGGTAG AACGAGGCAT CATCCCTAAT 
AAGAACAGAG CTGAGCAGCT AGCCAGTGTA CAGTATACAC TTCCAAAAAC AGCAGGCGGA 
GACCGTGCTG ACTTCTGGAG ATTCAGAGGT CTTCGCAGCT CCAAGAGAAA TCTTCGAAAA 
AGCAGAGAGG ATTTGTCCGC TCAGCCTGTT CAAACAAAGT TTCCAGCTTA TGAAAGAGTG 
GTTCTTCGAG AAGCTGGATT TCTGAGGCCT GTAACCATTT TTGGACCAAT AGCTGATGTT 
GCCAGAGAAA AGCTGGCAAG AGAAGAACCA GATATTTATC AAATTGCAAA GAGTGAACCA 
CGAGACGCTG GAACTGACCA ACGTAGCTCT GGCATTATTC GCCTGCATAC AATAAAGCAA 
ATCATAGATC AAGACAAACA TGCTTTATTA GATGTAACAC CAAATGCAGT TGATCGTCTT 
AACTATGCCC AGTGGTATCC AATTGTTGTA TTTCTTAACC CTGATTCTAA GCAAGGAGTA 
AAAACAATGA GAATGAGGTT ATGTCCAGAA TCTCGGAAAA GTGCCAGGAA GTTATATGAG 
CGATCTCATA AACTTCGTAA AAATAATCAC CATCTTTTTA CAACTACAAT TAACTTAAAT 
TCAATGAATG ATGGTTGGTA TGGTGCGCTG AAAGAAGCAA TTCAACAACA GCAAAACCAG 
CTGGTATGGG TTTCCGAGGG AAAGGCGGAT GGTGCTACAA GTGATGACCT TGATTTGCAT 
GATGATCGTC TGTCCTACCT GTCAGCTCCA GGTAGTGAAT ACTCAATGTA TAGCACGGAC 
AGTAGACACA CTTCTGACTA TGAAGACACA GACACAGAAG GCGGGGCCTA CACTGATCAA 
GAACTAGATG AAACTCTTAA TGATGAGGTT GGGACTCCAC CGGAGTCTGC CATTACACGG 
TCCTCTGAGC CTGTAAGAGA GGACTCCTCT GGAATGCATC ATGAAAACCA AACATATCCT 
CCTTACTCAC CACAAGCGCA GCCACAACCA ATTCATAGAA TAGACTCCCC TGGATTTAAG 
CCAGCCTCTC AACAGGTGTA TAGAAAGGAT CCATATCCCG AGGAAATGAT GAGGCAGAAC 
CATGTTTTGA AACAGCCAGC CGTTAGTCAC CCAGGGCACA GGCCAGACAA AGAGCCTAAT 
CTGACCTATG AACCCCAACT CCCATACGTA GAGAAACAAG CCAGCAGAGA CCTCGAGCAG 
CCCACATACA GATACGAGTC CTCAAGCTAT ACGGACCAGT TTTCTCGAAA CTATGAACAT 
CGTCTGCGAT ACGAAGATCG CGTCCCCATG TATGAAGAAC AGTGGTCATA TTATGATGAC 
AAACAGCCCT ACCCATCTCG GCCACCTTTT GATAATCAGC ACTCTCAAGA CCTTGACTCC 
AGACAGCATC CCGAAGAGTC CTCAGAACGA GGGTACTTTC CACGTTTTGA AGAGCCAGCC 
CCTCTGTCTT ACGACAGCAG ACCACGTTAC GAACAGGCAC CTAGAGCATC CGCCCTGCGG 
CACGAAGAGC AGCCAGCTCC TGGGTATGAC ACACATGGTA GACTCAGACC GGAAGCCCAG 
CCCCACCCTT CAGCAGGGCC CAAGCCCGCA GAGTCCAAGC AGTATTTTGA GCAATATTCA 
CGCAGTTACG AGCAAGTACC ACCCCAAGGA TTTACCTCTA GAGCAGGTCA TTTTGAGCCT 
CTCCATGGTG CTGCAGCTGT CCCTCCGCTG ATACCTTCAT CTCAGCATAA GCCAGAAGCT 
CTGCCTTCAA ACACCAAACC ACTGCCTCCA CCCCCAACTC AAACCGAAGA AGAGGAAGAT 
CCAGCAATGA AGCCACAGTC TGTACTCACC AGAGTTAAGA TGTTTGAAAA CAAAAGATCT 
GCATCCTTAG AGACCAAGAA GGATGTAAAT GACACTGGCA GTTTTAAGCC TCCAGAAGTA 
GCATCTAAAC CTTCAGGTGC TCCCATCATT GGTCCCAAAC CCACTTCTCA GAATCAGTTC 
AGTGAACATG ACAAAACTCT GTACAGGATC CCAGAACCTC AAAAACCTCA ACTGAAGCCA 
CCTGAAGATA TCGTTCGGTC CAATCATTAT GACCCTGAAG AAGATGAAGA ATATTATCGA 
AAACAGCTGT CATACTTTGA CCGAAGAAGT TTTGAGAATA AGCCTCCTGC ACACATTGCC 
GCCAGCCATC TCTCCGAGCC TGCAAAGCCA GCTCATTCTC AGAATCAATC AAATTTTTCT 
AGTTATTCTT CAAAGGGAAA GCCTCCTGAA GCTGATGGTG TGGATAGATC ATTTGGCGAG 
AAACGCTATG AACCCATCCA GGCCACTCCC CCTCCTCCTC CATTGCCCTC GCAGTATGCC 
CAGCCATCTC AGCCTGTCAC CAGCGCGTCT CTCCACATAC ATTCTAAGGG AGCACATGGT 
GAAGGTAATT CAGTGTCATT GGATTTTCAG AATTCCTTAG TGTCCAAACC AGACCCACCT 
CCATCTCAGA ATAAGCCAGC AACTTTCAGA CCACCAAACC GAGAAGATAC TGCTCAGCCA 
GCTTTCTATC CCCAGAAAAG TTTTCCAGAT AAAGCCCCAG TTAATGGAAC TGAACAGACT 
CAGAAAACAG TCACTCCAGC ATACAATCGA TTCACACCAA AACCATATAC AAGTTCTGCC 
CGACCATTTG AACGCAAGTT TGAAAGTCCT AAATTCAATC ACAATCTTCT GCCAAGTGAA 
ACTGCACATA AACCTGAATT GTCTTCAAAA ACTCCCACTT CTCCAAAAAC TCTTGTGAAA 
TCGCACAGTT TGGCACAGCC TCCTGAGTTT GACAGTGGAG TTGAAACTTT CTCTATCCAT 
GCAGAGAAGC CTAAATATCA AATAAATAAT ATCAGCACAG TGCCTAAAGC TATTCCTGTG 
AGTCCTTCAG CTGTGGAAGA GGATGAAGAT GAAGATGGTC ATACTGTGGT GGCCACAGCC 
CGAGGCATAT TTAACAGCAA TGGGGGCGTG CTGAGTTCCA TAGAAACTGG CGTTAGTATA 
ATTATCCCTC AAGGAGCCAT TCCCGAAGGA GTTGAGCAGG AAATCTATTT CAAGGTCTGC 
CGGGACAACA GCATCCTTCC ACCTTTAGAT AAAGAGAAAG GTGAAACACT GCTGAGTCCT 
TTGGTGATGT GTGGTCCCCA TGGCCTCAAG TTCCTGAAGC CTGTGGAGCT GCGCTTACCA 
CACTGTGCGT CCATGACTCC TGACGGTTGG TCTTTTGCTC TAAAATCATC CGACTCCTCG 
TCGGGTGATC CTAAAACCTG GCAAAACAAG TGTCTTCCCG GAGATCCAAA TTATCTCGTT 
GGAGCAAACT GTGTTTCTGT CCTTATTGAC CACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016783331.1
CDS	110..5188
Translation	MERNTFSMDCHSKSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQPAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPELSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_016927842.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes tight junction protein 1 (TJP1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016927842.2

ORF Insert Sequence:

ATGGAGAGAA ATACGTTTTC AATGGATTGC CACAGTAAGA GCACAGCAAT GGAGGAAACA 
GCTATATGGG AACAACATAC AGTGACGCTT CACAGGGCTC CTGGATTTGG GTTTGGAATT 
GCAATATCTG GTGGACGAGA TAATCCTCAT TTTCAGAGTG GGGAAACGTC AATAGTGATT 
TCAGATGTGC TGAAAGGAGG ACCAGCTGAA GGACAGCTAC AGGAAAATGA CCGAGTTGCA 
ATGGTTAACG GAGTTTCAAT GGATAATGTT GAACATGCTT TTGCTGTTCA GCAACTAAGG 
AAAAGTGGGA AAAATGCAAA AATTACAATT AGAAGGAAGA AGAAAGTTCA AATACCAGTA 
AGTCGTCCTG ATCCTGAACC AGTATCTGAT AATGAAGAAG ATAGTTATGA TGAGGAAATA 
CATGATCCAA GAAGTGGCCG GAGTGGTGTG GTTAACAGAA GGAGTGAGAA GATTTGGCCG 
AGGGATAGAA GTGCAAGTAG AGAGAGGAGC TTGTCCCCGC GGTCAGACAG GCGGTCAGTG 
GCTTCCAGCC AGCCCGCTAA ACCTACTAAA GTCACACTGG TGAAATCCCG GAAAAATGAA 
GAATATGGTC TTCGATTGGC AAGCCATATA TTTGTTAAGG AAATTTCACA AGATAGTTTG 
GCAGCAAGAG ATGGCAATAT TCAAGAAGGT GATGTTGTAT TGAAGATAAA TGGTACTGTG 
ACAGAAAATA TGTCATTGAC AGATGCAAAG ACATTGATAG AAAGGTCTAA AGGCAAATTA 
AAAATGGTAG TTCAAAGAGA TGAACGGGCT ACGCTATTGA ATGTCCCTGA TCTTTCTGAC 
AGCATCCACT CTGCTAATGC CTCTGAGAGA GACGACATTT CAGAAATTCA GTCACTGGCA 
TCAGATCATT CTGGTCGATC ACACGATAGG CCTCCCCGCC GCAGCCGGTC ACGATCTCCT 
GACCAGCGGT CAGAGCCTTC TGATCATTCC AGGCACTCGC CGCAGCAGCC AAGCAATGGC 
AGTCTCCGGA GTAGAGATGA AGAGAGAATT TCTAAACCTG GGGCTGTCTC AACTCCTGTA 
AAGCATGCTG ATGATCACAC ACCTAAAACA GTGGAAGAAG TTACAGTTGA AAGAAATGAG 
AAACAAACAC CTTCTCTTCC AGAACCAAAG CCTGTGTATG CCCAAGTTGG GCAGCCAGAT 
GTGGATTTAC CTGTCAGTCC ATCTGATGGT GTCCTACCTA ATTCAACTCA TGAAGATGGG 
ATTCTTCGGC CCAGCATGAA ATTGGTAAAA TTCAGAAAAG GAGATAGTGT GGGTTTGCGG 
CTGGCTGGTG GAAATGATGT TGGAATATTT GTAGCTGGCG TTCTAGAAGA TAGCCCTGCA 
GCCAAGGAAG GCTTAGAGGA AGGTGATCAA ATTCTCAGGG TAAACAACGT AGATTTTACA 
AATATCATAA GAGAAGAAGC CGTCCTTTTC CTGCTTGACC TCCCTAAAGG AGAAGAAGTG 
ACCATATTGG CTCAGAAGAA GAAGGATGTT TATCGTCGCA TTGTAGAATC AGATGTAGGA 
GATTCTTTCT ATATTAGAAC CCATTTTGAA TATGAAAAGG AATCTCCCTA TGGACTTAGT 
TTTAACAAAG GAGAGGTGTT CCGTGTTGTG GATACCTTGT ACAATGGAAA ACTGGGCTCT 
TGGCTTGCTA TTCGAATTGG TAAAAATCAT AAGGAGGTAG AACGAGGCAT CATCCCTAAT 
AAGAACAGAG CTGAGCAGCT AGCCAGTGTA CAGTATACAC TTCCAAAAAC AGCAGGCGGA 
GACCGTGCTG ACTTCTGGAG ATTCAGAGGT CTTCGCAGCT CCAAGAGAAA TCTTCGAAAA 
AGCAGAGAGG ATTTGTCCGC TCAGCCTGTT CAAACAAAGT TTCCAGCTTA TGAAAGAGTG 
GTTCTTCGAG AAGCTGGATT TCTGAGGCCT GTAACCATTT TTGGACCAAT AGCTGATGTT 
GCCAGAGAAA AGCTGGCAAG AGAAGAACCA GATATTTATC AAATTGCAAA GAGTGAACCA 
CGAGACGCTG GAACTGACCA ACGTAGCTCT GGCATTATTC GCCTGCATAC AATAAAGCAA 
ATCATAGATC AAGACAAACA TGCTTTATTA GATGTAACAC CAAATGCAGT TGATCGTCTT 
AACTATGCCC AGTGGTATCC AATTGTTGTA TTTCTTAACC CTGATTCTAA GCAAGGAGTA 
AAAACAATGA GAATGAGGTT ATGTCCAGAA TCTCGGAAAA GTGCCAGGAA GTTATATGAG 
CGATCTCATA AACTTCGTAA AAATAATCAC CATCTTTTTA CAACTACAAT TAACTTAAAT 
TCAATGAATG ATGGTTGGTA TGGTGCGCTG AAAGAAGCAA TTCAACAACA GCAAAACCAG 
CTGGTATGGG TTTCCGAGGG AAAGGCGGAT GGTGCTACAA GTGATGACCT TGATTTGCAT 
GATGATCGTC TGTCCTACCT GTCAGCTCCA GGTAGTGAAT ACTCAATGTA TAGCACGGAC 
AGTAGACACA CTTCTGACTA TGAAGACACA GACACAGAAG GCGGGGCCTA CACTGATCAA 
GAACTAGATG AAACTCTTAA TGATGAGGTT GGGACTCCAC CGGAGTCTGC CATTACACGG 
TCCTCTGAGC CTGTAAGAGA GGACTCCTCT GGAATGCATC ATGAAAACCA AACATATCCT 
CCTTACTCAC CACAAGCGCA GCCACAACCA ATTCATAGAA TAGACTCCCC TGGATTTAAG 
CCAGCCTCTC AACAGGTGTA TAGAAAGGAT CCATATCCCG AGGAAATGAT GAGGCAGAAC 
CATGTTTTGA AACAGCCAGC CGTTAGTCAC CCAGGGCACA GGCCAGACAA AGAGCCTAAT 
CTGACCTATG AACCCCAACT CCCATACGTA GAGAAACAAG CCAGCAGAGA CCTCGAGCAG 
CCCACATACA GATACGAGTC CTCAAGCTAT ACGGACCAGT TTTCTCGAAA CTATGAACAT 
CGTCTGCGAT ACGAAGATCG CGTCCCCATG TATGAAGAAC AGTGGTCATA TTATGATGAC 
AAACAGCCCT ACCCATCTCG GCCACCTTTT GATAATCAGC ACTCTCAAGA CCTTGACTCC 
AGACAGCATC CCGAAGAGTC CTCAGAACGA GGGTACTTTC CACGTTTTGA AGAGCCAGCC 
CCTCTGTCTT ACGACAGCAG ACCACGTTAC GAACAGGCAC CTAGAGCATC CGCCCTGCGG 
CACGAAGAGC AGCCAGCTCC TGGGTATGAC ACACATGGTA GACTCAGACC GGAAGCCCAG 
CCCCACCCTT CAGCAGGGCC CAAGCCCGCA GAGTCCAAGC AGTATTTTGA GCAATATTCA 
CGCAGTTACG AGCAAGTACC ACCCCAAGGA TTTACCTCTA GAGCAGGTCA TTTTGAGCCT 
CTCCATGGTG CTGCAGCTGT CCCTCCGCTG ATACCTTCAT CTCAGCATAA GCCAGAAGCT 
CTGCCTTCAA ACACCAAACC ACTGCCTCCA CCCCCAACTC AAACCGAAGA AGAGGAAGAT 
CCAGCAATGA AGCCACAGTC TGTACTCACC AGAGTTAAGA TGTTTGAAAA CAAAAGATCT 
GCATCCTTAG AGACCAAGAA GGATGTAAAT GACACTGGCA GTTTTAAGCC TCCAGAAGTA 
GCATCTAAAC CTTCAGGTGC TCCCATCATT GGTCCCAAAC CCACTTCTCA GAATCAGTTC 
AGTGAACATG ACAAAACTCT GTACAGGATC CCAGAACCTC AAAAACCTCA ACTGAAGCCA 
CCTGAAGATA TCGTTCGGTC CAATCATTAT GACCCTGAAG AAGATGAAGA ATATTATCGA 
AAACAGCTGT CATACTTTGA CCGAAGAAGT TTTGAGAATA AGCCTCCTGC ACACATTGCC 
GCCAGCCATC TCTCCGAGCC TGCAAAGCCA GCTCATTCTC AGAATCAATC AAATTTTTCT 
AGTTATTCTT CAAAGGGAAA GCCTCCTGAA GCTGATGGTG TGGATAGATC ATTTGGCGAG 
AAACGCTATG AACCCATCCA GGCCACTCCC CCTCCTCCTC CATTGCCCTC GCAGTATGCC 
CAGCCATCTC AGCCTGTCAC CAGCGCGTCT CTCCACATAC ATTCTAAGGG AGCACATGGT 
GAAGGTAATT CAGTGTCATT GGATTTTCAG AATTCCTTAG TGTCCAAACC AGACCCACCT 
CCATCTCAGA ATAAGCCAGC AACTTTCAGA CCACCAAACC GAGAAGATAC TGCTCAGCCA 
GCTTTCTATC CCCAGAAAAG TTTTCCAGAT AAAGCCCCAG TTAATGGAAC TGAACAGACT 
CAGAAAACAG TCACTCCAGC ATACAATCGA TTCACACCAA AACCATATAC AAGTTCTGCC 
CGACCATTTG AACGCAAGTT TGAAAGTCCT AAATTCAATC ACAATCTTCT GCCAAGTGAA 
ACTGCACATA AACCTGAATT GTCTTCAAAA ACTCCCACTT CTCCAAAAAC TCTTGTGAAA 
TCGCACAGTT TGGCACAGCC TCCTGAGTTT GACAGTGGAG TTGAAACTTT CTCTATCCAT 
GCAGAGAAGC CTAAATATCA AATAAATAAT ATCAGCACAG TGCCTAAAGC TATTCCTGTG 
AGTCCTTCAG CTGTGGAAGA GGATGAAGAT GAAGATGGTC ATACTGTGGT GGCCACAGCC 
CGAGGCATAT TTAACAGCAA TGGGGGCGTG CTGAGTTCCA TAGAAACTGG CGTTAGTATA 
ATTATCCCTC AAGGAGCCAT TCCCGAAGGA GTTGAGCAGG AAATCTATTT CAAGGTCTGC 
CGGGACAACA GCATCCTTCC ACCTTTAGAT AAAGAGAAAG GTGAAACACT GCTGAGTCCT 
TTGGTGATGT GTGGTCCCCA TGGCCTCAAG TTCCTGAAGC CTGTGGAGCT GCGCTTACCA 
CACTGTGCGT CCATGACTCC TGACGGTTGG TCTTTTGCTC TAAAATCATC CGACTCCTCG 
TCGGGTGATC CTAAAACCTG GCAAAACAAG TGTCTTCCCG GAGATCCAAA TTATCTCGTT 
GGAGCAAACT GTGTTTCTGT CCTTATTGAC CACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_024349009.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_024204777.1 Pan troglodytes tight junction protein 1 (TJP1), transcript variant X14, mRNA.

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CloneID	OPc97839
Clone ID Related Accession (Same CDS sequence)	XM_024349009.1
Accession Version	XM_024349009.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5067bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	tight junction protein ZO-1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036894.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGGT TTGGAATTGC AATATCTGGT 
GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG 
AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA 
GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT 
CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA 
AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT 
GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG 
CCCGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT 
CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT 
GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT 
GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA 
GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT 
AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT 
GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT 
TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AGCCAGATGT GGATTTACCT 
GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC 
AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA 
AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA 
GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT 
CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC 
TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT 
TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA 
GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA 
GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG 
TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA 
ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATATGAGCG ATCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT 
TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA 
CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA 
CAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA 
CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA 
CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA 
TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC 
GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC 
GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC 
GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG 
CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA 
GCAGGGCCCA AGCCCGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG 
CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT 
GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC 
ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG 
CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG 
ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT 
TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAGTTCAG TGAACATGAC 
AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATC 
GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA 
TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC 
TCCGAGCCTG CAAAGCCAGC TCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA 
AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA 
CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG 
CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA 
GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT 
AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGCCAGC TTTCTATCCC 
CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC 
ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA 
CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA 
CCTGAATTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG 
GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT 
AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT 
GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT 
AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGCG TTAGTATAAT TATCCCTCAA 
GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC 
ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT 
GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGCGTCC 
ATGACTCCTG ACGGTTGGTC TTTTGCTCTA AAATCATCCG ACTCCTCGTC GGGTGATCCT 
AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT 
GTTTCTGTCC TTATTGACCA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024204777.1
CDS	476..5542
Translation	MSARAAAAKSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQPAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPELSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_024349009.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes tight junction protein 1 (TJP1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024349009.1

ORF Insert Sequence:

ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGGT TTGGAATTGC AATATCTGGT 
GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG 
AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA 
GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT 
CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA 
AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT 
GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG 
CCCGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT 
CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT 
GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT 
GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA 
GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT 
AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT 
GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT 
TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AGCCAGATGT GGATTTACCT 
GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC 
AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA 
AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA 
GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT 
CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC 
TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT 
TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA 
GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA 
GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG 
TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA 
ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATATGAGCG ATCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT 
TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA 
CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA 
CAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA 
CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA 
CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA 
TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC 
GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC 
GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC 
GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG 
CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA 
GCAGGGCCCA AGCCCGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG 
CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT 
GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC 
ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG 
CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG 
ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT 
TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAGTTCAG TGAACATGAC 
AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATC 
GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA 
TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC 
TCCGAGCCTG CAAAGCCAGC TCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA 
AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA 
CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG 
CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA 
GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT 
AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGCCAGC TTTCTATCCC 
CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC 
ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA 
CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA 
CCTGAATTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG 
GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT 
AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT 
GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT 
AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGCG TTAGTATAAT TATCCCTCAA 
GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC 
ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT 
GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGCGTCC 
ATGACTCCTG ACGGTTGGTC TTTTGCTCTA AAATCATCCG ACTCCTCGTC GGGTGATCCT 
AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT 
GTTTCTGTCC TTATTGACCA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_001163045.4 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_001163045.2 Pan troglodytes tight junction protein 1 (TJP1), transcript variant X15, mRNA.

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CloneID	OPc97840
Clone ID Related Accession (Same CDS sequence)	XM_001163045.4
Accession Version	XM_001163045.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	tight junction protein ZO-1 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036894.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_001163045.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGGT TTGGAATTGC AATATCTGGT 
GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG 
AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA 
GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT 
CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA 
AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT 
GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG 
CCCGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT 
CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT 
GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT 
GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA 
GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT 
AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT 
GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT 
TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AGCCAGATGT GGATTTACCT 
GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC 
AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA 
AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA 
GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT 
CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC 
TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT 
TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA 
GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA 
GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG 
TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA 
ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATATGAGCG ATCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT 
TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA 
CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA 
CAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA 
CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA 
CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA 
TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC 
GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC 
GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC 
GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG 
CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA 
GCAGGGCCCA AGCCCGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG 
CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT 
GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC 
ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG 
CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG 
ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT 
TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAGTTCAG TGAACATGAC 
AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATC 
GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA 
TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC 
TCCGAGCCTG CAAAGCCAGC TCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA 
AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA 
CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG 
CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA 
GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT 
AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGCCAGC TTTCTATCCC 
CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC 
ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA 
CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA 
CCTGAATTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG 
GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT 
AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT 
GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT 
AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGCG TTAGTATAAT TATCCCTCAA 
GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC 
ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT 
GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGATCCT 
AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT 
GTTTCTGTCC TTATTGACCA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001163045.2
CDS	476..5482
Translation	MSARAAAAKSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQPAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPELSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_001163045.4
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes tight junction protein 1 (TJP1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001163045.4

ORF Insert Sequence:

ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGGT TTGGAATTGC AATATCTGGT 
GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG 
AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA 
GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT 
CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA 
AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT 
GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG 
CCCGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT 
CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT 
GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT 
GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA 
GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT 
AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT 
GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT 
TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AGCCAGATGT GGATTTACCT 
GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC 
AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA 
AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA 
GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT 
CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC 
TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT 
TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA 
GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA 
GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG 
TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA 
ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATATGAGCG ATCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT 
TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA 
CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA 
CAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA 
CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA 
CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA 
TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC 
GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC 
GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC 
GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG 
CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA 
GCAGGGCCCA AGCCCGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG 
CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT 
GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC 
ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG 
CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG 
ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT 
TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAGTTCAG TGAACATGAC 
AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATC 
GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA 
TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC 
TCCGAGCCTG CAAAGCCAGC TCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA 
AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA 
CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG 
CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA 
GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT 
AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGCCAGC TTTCTATCCC 
CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC 
ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA 
CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA 
CCTGAATTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG 
GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT 
AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT 
GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT 
AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGCG TTAGTATAAT TATCCCTCAA 
GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC 
ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT 
GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGATCCT 
AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT 
GTTTCTGTCC TTATTGACCA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_016927843.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016783332.1 Pan troglodytes tight junction protein 1 (TJP1), transcript variant X16, mRNA.

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CloneID	OPc97841
Clone ID Related Accession (Same CDS sequence)	XM_016927843.2
Accession Version	XM_016927843.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4620bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	tight junction protein ZO-1 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036894.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016927843.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATAA ATGGTACTGT GACAGAAAAT ATGTCATTGA CAGATGCAAA GACATTGATA 
GAAAGGTCTA AAGGCAAATT AAAAATGGTA GTTCAAAGAG ATGAACGGGC TACGCTATTG 
AATGTCCCTG ATCTTTCTGA CAGCATCCAC TCTGCTAATG CCTCTGAGAG AGACGACATT 
TCAGAAATTC AGTCACTGGC ATCAGATCAT TCTGGTCGAT CACACGATAG GCCTCCCCGC 
CGCAGCCGGT CACGATCTCC TGACCAGCGG TCAGAGCCTT CTGATCATTC CAGGCACTCG 
CCGCAGCAGC CAAGCAATGG CAGTCTCCGG AGTAGAGATG AAGAGAGAAT TTCTAAACCT 
GGGGCTGTCT CAACTCCTGT AAAGCATGCT GATGATCACA CACCTAAAAC AGTGGAAGAA 
GTTACAGTTG AAAGAAATGA GAAACAAACA CCTTCTCTTC CAGAACCAAA GCCTGTGTAT 
GCCCAAGTTG GGCAGCCAGA TGTGGATTTA CCTGTCAGTC CATCTGATGG TGTCCTACCT 
AATTCAACTC ATGAAGATGG GATTCTTCGG CCCAGCATGA AATTGGTAAA ATTCAGAAAA 
GGAGATAGTG TGGGTTTGCG GCTGGCTGGT GGAAATGATG TTGGAATATT TGTAGCTGGC 
GTTCTAGAAG ATAGCCCTGC AGCCAAGGAA GGCTTAGAGG AAGGTGATCA AATTCTCAGG 
GTAAACAACG TAGATTTTAC AAATATCATA AGAGAAGAAG CCGTCCTTTT CCTGCTTGAC 
CTCCCTAAAG GAGAAGAAGT GACCATATTG GCTCAGAAGA AGAAGGATGT TTATCGTCGC 
ATTGTAGAAT CAGATGTAGG AGATTCTTTC TATATTAGAA CCCATTTTGA ATATGAAAAG 
GAATCTCCCT ATGGACTTAG TTTTAACAAA GGAGAGGTGT TCCGTGTTGT GGATACCTTG 
TACAATGGAA AACTGGGCTC TTGGCTTGCT ATTCGAATTG GTAAAAATCA TAAGGAGGTA 
GAACGAGGCA TCATCCCTAA TAAGAACAGA GCTGAGCAGC TAGCCAGTGT ACAGTATACA 
CTTCCAAAAA CAGCAGGCGG AGACCGTGCT GACTTCTGGA GATTCAGAGG TCTTCGCAGC 
TCCAAGAGAA ATCTTCGAAA AAGCAGAGAG GATTTGTCCG CTCAGCCTGT TCAAACAAAG 
TTTCCAGCTT ATGAAAGAGT GGTTCTTCGA GAAGCTGGAT TTCTGAGGCC TGTAACCATT 
TTTGGACCAA TAGCTGATGT TGCCAGAGAA AAGCTGGCAA GAGAAGAACC AGATATTTAT 
CAAATTGCAA AGAGTGAACC ACGAGACGCT GGAACTGACC AACGTAGCTC TGGCATTATT 
CGCCTGCATA CAATAAAGCA AATCATAGAT CAAGACAAAC ATGCTTTATT AGATGTAACA 
CCAAATGCAG TTGATCGTCT TAACTATGCC CAGTGGTATC CAATTGTTGT ATTTCTTAAC 
CCTGATTCTA AGCAAGGAGT AAAAACAATG AGAATGAGGT TATGTCCAGA ATCTCGGAAA 
AGTGCCAGGA AGTTATATGA GCGATCTCAT AAACTTCGTA AAAATAATCA CCATCTTTTT 
ACAACTACAA TTAACTTAAA TTCAATGAAT GATGGTTGGT ATGGTGCGCT GAAAGAAGCA 
ATTCAACAAC AGCAAAACCA GCTGGTATGG GTTTCCGAGG GAAAGGCGGA TGGTGCTACA 
AGTGATGACC TTGATTTGCA TGATGATCGT CTGTCCTACC TGTCAGCTCC AGGTAGTGAA 
TACTCAATGT ATAGCACGGA CAGTAGACAC ACTTCTGACT ATGAAGACAC AGACACAGAA 
GGCGGGGCCT ACACTGATCA AGAACTAGAT GAAACTCTTA ATGATGAGGT TGGGACTCCA 
CCGGAGTCTG CCATTACACG GTCCTCTGAG CCTGTAAGAG AGGACTCCTC TGGAATGCAT 
CATGAAAACC AAACATATCC TCCTTACTCA CCACAAGCGC AGCCACAACC AATTCATAGA 
ATAGACTCCC CTGGATTTAA GCCAGCCTCT CAACAGAAAG CAGAAGCTTC ATCTCCAGTC 
CCTTACCTTT CGCCTGAAAC AAACCCAGCA TCATCAACCT CTGCTGTTAA TCATAATGTA 
AATTTAACTA ATGTCAGACT GGAGGAGCCC ACCCCAGCTC CTTCCACCTC TTACTCACCA 
CAAGCTGATT CTTTAAGAAC ACCAAGTACT GAGGCAGCTC ACATAATGCT AAGAGATCAA 
GAACCATCAT TGTCGTCGCA TGTAGATCCA ACAAAGGTGT ATAGAAAGGA TCCATATCCC 
GAGGAAATGA TGAGGCAGAA CCATGTTTTG AAACAGCCAG CCGTTAGTCA CCCAGGGCAC 
AGGCCAGACA AAGAGCCTAA TCTGACCTAT GAACCCCAAC TCCCATACGT AGAGAAACAA 
GCCAGCAGAG ACCTCGAGCA GCCCACATAC AGATACGAGT CCTCAAGCTA TACGGACCAG 
TTTTCTCGAA ACTATGAACA TCGTCTGCGA TACGAAGATC GCGTCCCCAT GTATGAAGAA 
CAGTGGTCAT ATTATGATGA CAAACAGCCC TACCCATCTC GGCCACCTTT TGATAATCAG 
CACTCTCAAG ACCTTGACTC CAGACAGCAT CCCGAAGAGT CCTCAGAACG AGGGTACTTT 
CCACGTTTTG AAGAGCCAGC CCCTCTGTCT TACGACAGCA GACCACGTTA CGAACAGGCA 
CCTAGAGCAT CCGCCCTGCG GCACGAAGAG CAGCCAGCTC CTGGGTATGA CACACATGGT 
AGACTCAGAC CGGAAGCCCA GCCCCACCCT TCAGCAGGGC CCAAGCCCGC AGAGTCCAAG 
CAGTATTTTG AGCAATATTC ACGCAGTTAC GAGCAAGTAC CACCCCAAGG ATTTACCTCT 
AGAGCAGGTC ATTTTGAGCC TCTCCATGGT GCTGCAGCTG TCCCTCCGCT GATACCTTCA 
TCTCAGCATA AGCCAGAAGC TCTGCCTTCA AACACCAAAC CACTGCCTCC ACCCCCAACT 
CAAACCGAAG AAGAGGAAGA TCCAGCAATG AAGCCACAGT CTGTACTCAC CAGAGTTAAG 
ATGTTTGAAA ACAAAAGATC TGCATCCTTA GAGACCAAGA AGGATGTAAA TGACACTGGC 
AGTTTTAAGC CTCCAGAAGT AGCATCTAAA CCTTCAGGTG CTCCCATCAT TGGTCCCAAA 
CCCACTTCTC AGAATCAGTT CAGTGAACAT GACAAAACTC TGTACAGGAT CCCAGAACCT 
CAAAAACCTC AACTGAAGCC ACCTGAAGAT ATCGTTCGGT CCAATCATTA TGACCCTGAA 
GAAGATGAAG AATATTATCG AAAACAGCTG TCATACTTTG ACCGAAGAAG TTTTGAGAAT 
AAGCCTCCTG CACACATTGC CGCCAGCCAT CTCTCCGAGC CTGCAAAGCC AGCTCATTCT 
CAGAATCAAT CAAATTTTTC TAGTTATTCT TCAAAGGGAA AGCCTCCTGA AGCTGATGGT 
GTGGATAGAT CATTTGGCGA GAAACGCTAT GAACCCATCC AGGCCACTCC CCCTCCTCCT 
CCATTGCCCT CGCAGTATGC CCAGCCATCT CAGCCTGTCA CCAGCGCGTC TCTCCACATA 
CATTCTAAGG GAGCACATGG TGAAGGTAAT TCAGTGTCAT TGGATTTTCA GAATTCCTTA 
GTGTCCAAAC CAGACCCACC TCCATCTCAG AATAAGCCAG CAACTTTCAG ACCACCAAAC 
CGAGAAGATA CTGCTCAGCC AGCTTTCTAT CCCCAGAAAA GTTTTCCAGA TAAAGCCCCA 
GTTAATGGAA CTGAACAGAC TCAGAAAACA GTCACTCCAG CATACAATCG ATTCACACCA 
AAACCATATA CAAGTTCTGC CCGACCATTT GAACGCAAGT TTGAAAGTCC TAAATTCAAT 
CACAATCTTC TGCCAAGTGA AACTGCACAT AAACCTGAAT TGTCTTCAAA AACTCCCACT 
TCTCCAAAAA CTCTTGTGAA ATCGCACAGT TTGGCACAGC CTCCTGAGTT TGACAGTGGA 
GTTGAAACTT TCTCTATCCA TGCAGAGAAG CCTAAATATC AAATAAATAA TATCAGCACA 
GTGCCTAAAG CTATTCCTGT GAGTCCTTCA GCTGTGGAAG AGGATGAAGA TGAAGATGGT 
CATACTGTGG TGGCCACAGC CCGAGGCATA TTTAACAGCA ATGGGGGCGT GCTGAGTTCC 
ATAGAAACTG GCGTTAGTAT AATTATCCCT CAAGGAGCCA TTCCCGAAGG AGTTGAGCAG 
GAAATCTATT TCAAGGTCTG CCGGGACAAC AGCATCCTTC CACCTTTAGA TAAAGAGAAA 
GGTGAAACAC TGCTGAGTCC TTTGGTGATG TGTGGTCCCC ATGGCCTCAA GTTCCTGAAG 
CCTGTGGAGC TGCGCTTACC ACACTGTGCG TCCATGACTC CTGACGGTTG GTCTTTTGCT 
CTAAAATCAT CCGACTCCTC GTCGGGTGAT CCTAAAACCT GGCAAAACAA GTGTCTTCCC 
GGAGATCCAA ATTATCTCGT TGGAGCAAAC TGTGTTTCTG TCCTTATTGA CCACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016783332.1
CDS	89..4708
Translation	MKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQKAEASSPVPYLSPETNPASSTSAVNHNVNLTNVRLEEPTPAPSTSYSPQADSLRTPSTEAAHIMLRDQEPSLSSHVDPTKVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQPAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPELSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_016927843.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes tight junction protein 1 (TJP1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016927843.2

ORF Insert Sequence:

ATGAAGATAA ATGGTACTGT GACAGAAAAT ATGTCATTGA CAGATGCAAA GACATTGATA 
GAAAGGTCTA AAGGCAAATT AAAAATGGTA GTTCAAAGAG ATGAACGGGC TACGCTATTG 
AATGTCCCTG ATCTTTCTGA CAGCATCCAC TCTGCTAATG CCTCTGAGAG AGACGACATT 
TCAGAAATTC AGTCACTGGC ATCAGATCAT TCTGGTCGAT CACACGATAG GCCTCCCCGC 
CGCAGCCGGT CACGATCTCC TGACCAGCGG TCAGAGCCTT CTGATCATTC CAGGCACTCG 
CCGCAGCAGC CAAGCAATGG CAGTCTCCGG AGTAGAGATG AAGAGAGAAT TTCTAAACCT 
GGGGCTGTCT CAACTCCTGT AAAGCATGCT GATGATCACA CACCTAAAAC AGTGGAAGAA 
GTTACAGTTG AAAGAAATGA GAAACAAACA CCTTCTCTTC CAGAACCAAA GCCTGTGTAT 
GCCCAAGTTG GGCAGCCAGA TGTGGATTTA CCTGTCAGTC CATCTGATGG TGTCCTACCT 
AATTCAACTC ATGAAGATGG GATTCTTCGG CCCAGCATGA AATTGGTAAA ATTCAGAAAA 
GGAGATAGTG TGGGTTTGCG GCTGGCTGGT GGAAATGATG TTGGAATATT TGTAGCTGGC 
GTTCTAGAAG ATAGCCCTGC AGCCAAGGAA GGCTTAGAGG AAGGTGATCA AATTCTCAGG 
GTAAACAACG TAGATTTTAC AAATATCATA AGAGAAGAAG CCGTCCTTTT CCTGCTTGAC 
CTCCCTAAAG GAGAAGAAGT GACCATATTG GCTCAGAAGA AGAAGGATGT TTATCGTCGC 
ATTGTAGAAT CAGATGTAGG AGATTCTTTC TATATTAGAA CCCATTTTGA ATATGAAAAG 
GAATCTCCCT ATGGACTTAG TTTTAACAAA GGAGAGGTGT TCCGTGTTGT GGATACCTTG 
TACAATGGAA AACTGGGCTC TTGGCTTGCT ATTCGAATTG GTAAAAATCA TAAGGAGGTA 
GAACGAGGCA TCATCCCTAA TAAGAACAGA GCTGAGCAGC TAGCCAGTGT ACAGTATACA 
CTTCCAAAAA CAGCAGGCGG AGACCGTGCT GACTTCTGGA GATTCAGAGG TCTTCGCAGC 
TCCAAGAGAA ATCTTCGAAA AAGCAGAGAG GATTTGTCCG CTCAGCCTGT TCAAACAAAG 
TTTCCAGCTT ATGAAAGAGT GGTTCTTCGA GAAGCTGGAT TTCTGAGGCC TGTAACCATT 
TTTGGACCAA TAGCTGATGT TGCCAGAGAA AAGCTGGCAA GAGAAGAACC AGATATTTAT 
CAAATTGCAA AGAGTGAACC ACGAGACGCT GGAACTGACC AACGTAGCTC TGGCATTATT 
CGCCTGCATA CAATAAAGCA AATCATAGAT CAAGACAAAC ATGCTTTATT AGATGTAACA 
CCAAATGCAG TTGATCGTCT TAACTATGCC CAGTGGTATC CAATTGTTGT ATTTCTTAAC 
CCTGATTCTA AGCAAGGAGT AAAAACAATG AGAATGAGGT TATGTCCAGA ATCTCGGAAA 
AGTGCCAGGA AGTTATATGA GCGATCTCAT AAACTTCGTA AAAATAATCA CCATCTTTTT 
ACAACTACAA TTAACTTAAA TTCAATGAAT GATGGTTGGT ATGGTGCGCT GAAAGAAGCA 
ATTCAACAAC AGCAAAACCA GCTGGTATGG GTTTCCGAGG GAAAGGCGGA TGGTGCTACA 
AGTGATGACC TTGATTTGCA TGATGATCGT CTGTCCTACC TGTCAGCTCC AGGTAGTGAA 
TACTCAATGT ATAGCACGGA CAGTAGACAC ACTTCTGACT ATGAAGACAC AGACACAGAA 
GGCGGGGCCT ACACTGATCA AGAACTAGAT GAAACTCTTA ATGATGAGGT TGGGACTCCA 
CCGGAGTCTG CCATTACACG GTCCTCTGAG CCTGTAAGAG AGGACTCCTC TGGAATGCAT 
CATGAAAACC AAACATATCC TCCTTACTCA CCACAAGCGC AGCCACAACC AATTCATAGA 
ATAGACTCCC CTGGATTTAA GCCAGCCTCT CAACAGAAAG CAGAAGCTTC ATCTCCAGTC 
CCTTACCTTT CGCCTGAAAC AAACCCAGCA TCATCAACCT CTGCTGTTAA TCATAATGTA 
AATTTAACTA ATGTCAGACT GGAGGAGCCC ACCCCAGCTC CTTCCACCTC TTACTCACCA 
CAAGCTGATT CTTTAAGAAC ACCAAGTACT GAGGCAGCTC ACATAATGCT AAGAGATCAA 
GAACCATCAT TGTCGTCGCA TGTAGATCCA ACAAAGGTGT ATAGAAAGGA TCCATATCCC 
GAGGAAATGA TGAGGCAGAA CCATGTTTTG AAACAGCCAG CCGTTAGTCA CCCAGGGCAC 
AGGCCAGACA AAGAGCCTAA TCTGACCTAT GAACCCCAAC TCCCATACGT AGAGAAACAA 
GCCAGCAGAG ACCTCGAGCA GCCCACATAC AGATACGAGT CCTCAAGCTA TACGGACCAG 
TTTTCTCGAA ACTATGAACA TCGTCTGCGA TACGAAGATC GCGTCCCCAT GTATGAAGAA 
CAGTGGTCAT ATTATGATGA CAAACAGCCC TACCCATCTC GGCCACCTTT TGATAATCAG 
CACTCTCAAG ACCTTGACTC CAGACAGCAT CCCGAAGAGT CCTCAGAACG AGGGTACTTT 
CCACGTTTTG AAGAGCCAGC CCCTCTGTCT TACGACAGCA GACCACGTTA CGAACAGGCA 
CCTAGAGCAT CCGCCCTGCG GCACGAAGAG CAGCCAGCTC CTGGGTATGA CACACATGGT 
AGACTCAGAC CGGAAGCCCA GCCCCACCCT TCAGCAGGGC CCAAGCCCGC AGAGTCCAAG 
CAGTATTTTG AGCAATATTC ACGCAGTTAC GAGCAAGTAC CACCCCAAGG ATTTACCTCT 
AGAGCAGGTC ATTTTGAGCC TCTCCATGGT GCTGCAGCTG TCCCTCCGCT GATACCTTCA 
TCTCAGCATA AGCCAGAAGC TCTGCCTTCA AACACCAAAC CACTGCCTCC ACCCCCAACT 
CAAACCGAAG AAGAGGAAGA TCCAGCAATG AAGCCACAGT CTGTACTCAC CAGAGTTAAG 
ATGTTTGAAA ACAAAAGATC TGCATCCTTA GAGACCAAGA AGGATGTAAA TGACACTGGC 
AGTTTTAAGC CTCCAGAAGT AGCATCTAAA CCTTCAGGTG CTCCCATCAT TGGTCCCAAA 
CCCACTTCTC AGAATCAGTT CAGTGAACAT GACAAAACTC TGTACAGGAT CCCAGAACCT 
CAAAAACCTC AACTGAAGCC ACCTGAAGAT ATCGTTCGGT CCAATCATTA TGACCCTGAA 
GAAGATGAAG AATATTATCG AAAACAGCTG TCATACTTTG ACCGAAGAAG TTTTGAGAAT 
AAGCCTCCTG CACACATTGC CGCCAGCCAT CTCTCCGAGC CTGCAAAGCC AGCTCATTCT 
CAGAATCAAT CAAATTTTTC TAGTTATTCT TCAAAGGGAA AGCCTCCTGA AGCTGATGGT 
GTGGATAGAT CATTTGGCGA GAAACGCTAT GAACCCATCC AGGCCACTCC CCCTCCTCCT 
CCATTGCCCT CGCAGTATGC CCAGCCATCT CAGCCTGTCA CCAGCGCGTC TCTCCACATA 
CATTCTAAGG GAGCACATGG TGAAGGTAAT TCAGTGTCAT TGGATTTTCA GAATTCCTTA 
GTGTCCAAAC CAGACCCACC TCCATCTCAG AATAAGCCAG CAACTTTCAG ACCACCAAAC 
CGAGAAGATA CTGCTCAGCC AGCTTTCTAT CCCCAGAAAA GTTTTCCAGA TAAAGCCCCA 
GTTAATGGAA CTGAACAGAC TCAGAAAACA GTCACTCCAG CATACAATCG ATTCACACCA 
AAACCATATA CAAGTTCTGC CCGACCATTT GAACGCAAGT TTGAAAGTCC TAAATTCAAT 
CACAATCTTC TGCCAAGTGA AACTGCACAT AAACCTGAAT TGTCTTCAAA AACTCCCACT 
TCTCCAAAAA CTCTTGTGAA ATCGCACAGT TTGGCACAGC CTCCTGAGTT TGACAGTGGA 
GTTGAAACTT TCTCTATCCA TGCAGAGAAG CCTAAATATC AAATAAATAA TATCAGCACA 
GTGCCTAAAG CTATTCCTGT GAGTCCTTCA GCTGTGGAAG AGGATGAAGA TGAAGATGGT 
CATACTGTGG TGGCCACAGC CCGAGGCATA TTTAACAGCA ATGGGGGCGT GCTGAGTTCC 
ATAGAAACTG GCGTTAGTAT AATTATCCCT CAAGGAGCCA TTCCCGAAGG AGTTGAGCAG 
GAAATCTATT TCAAGGTCTG CCGGGACAAC AGCATCCTTC CACCTTTAGA TAAAGAGAAA 
GGTGAAACAC TGCTGAGTCC TTTGGTGATG TGTGGTCCCC ATGGCCTCAA GTTCCTGAAG 
CCTGTGGAGC TGCGCTTACC ACACTGTGCG TCCATGACTC CTGACGGTTG GTCTTTTGCT 
CTAAAATCAT CCGACTCCTC GTCGGGTGAT CCTAAAACCT GGCAAAACAA GTGTCTTCCC 
GGAGATCCAA ATTATCTCGT TGGAGCAAAC TGTGTTTCTG TCCTTATTGA CCACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_009428684.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009426959.2 Pan troglodytes tight junction protein 1 (TJP1), transcript variant X1, mRNA.

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CloneID	OPc69374
Clone ID Related Accession (Same CDS sequence)	XM_009428684.2
Accession Version	XM_009428684.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5586bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	tight junction protein ZO-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006482.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009428684.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG 
CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA 
AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC 
AGGGCTCCTG GATTTGGGTT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA 
CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA 
AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT 
GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT 
AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG 
TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CCGCTAAACC TACTAAAGTC 
ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT 
GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT 
GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA 
TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG 
CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC 
GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT 
CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG 
CACTCGCCAC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT 
AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG 
GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT 
GTGTATGCCC AAGTTGGGCA GCCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC 
CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG 
CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT 
CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT 
GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT 
ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG 
TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA 
ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA 
ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT 
ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC 
ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT 
GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT 
CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT 
CGGAAAAGTG CCAGGAAGTT ATATGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT 
CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA 
GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT 
AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG 
ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA 
ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT 
CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCTTCATCT 
CCAGTCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAT CAACCTCTGC TGTTAATCAT 
AATGTAAATT TAACTAATGT CAGACTGGAG GAGCCCACCC CAGCTCCTTC CACCTCTTAC 
TCACCACAAG CTGATTCTTT AAGAACACCA AGTACTGAGG CAGCTCACAT AATGCTAAGA 
GATCAAGAAC CATCATTGTC GTCGCATGTA GATCCAACAA AGGTGTATAG AAAGGATCCA 
TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA 
GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG 
AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG 
GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT 
GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT 
AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG 
TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA 
CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA 
CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCCGCAGAG 
TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT 
ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA 
CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC 
CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA 
GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC 
ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT 
CCCAAACCCA CTTCTCAGAA TCAGTTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA 
GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATCG TTCGGTCCAA TCATTATGAC 
CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT 
GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCT 
CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT 
GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT 
CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC 
CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT 
TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA 
CCAAACCGAG AAGATACTGC TCAGCCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA 
GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC 
ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA 
TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGAATTGTC TTCAAAAACT 
CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC 
AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC 
AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA 
GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG 
AGTTCCATAG AAACTGGCGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT 
GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA 
GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC 
CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGCGTCCA TGACTCCTGA CGGTTGGTCT 
TTTGCTCTAA AATCATCCGA CTCCTCGTCG GGTGATCCTA AAACCTGGCA AAACAAGTGT 
CTTCCCGGAG ATCCAAATTA TCTCGTTGGA GCAAACTGTG TTTCTGTCCT TATTGACCAC 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009426959.2
CDS	18..5603
Translation	MRRLEGLCLRRRRRRRGPARERSGDKMKYQKYLTVLQMAIGVTPSNRGSLLPLKRKLWVTPSSENPNGATSSVSQGKPSLRRIKGRLHRSKSLDSMDFCELTSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQKAEASSPVPYLSPETNPASSTSAVNHNVNLTNVRLEEPTPAPSTSYSPQADSLRTPSTEAAHIMLRDQEPSLSSHVDPTKVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQPAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPELSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_009428684.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes tight junction protein 1 (TJP1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009428684.2

ORF Insert Sequence:

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG 
CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA 
AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC 
AGGGCTCCTG GATTTGGGTT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA 
CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA 
AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT 
GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT 
AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG 
TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CCGCTAAACC TACTAAAGTC 
ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT 
GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT 
GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA 
TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG 
CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC 
GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT 
CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG 
CACTCGCCAC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT 
AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG 
GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT 
GTGTATGCCC AAGTTGGGCA GCCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC 
CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG 
CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT 
CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT 
GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT 
ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG 
TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA 
ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA 
ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT 
ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC 
ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT 
GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT 
CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT 
CGGAAAAGTG CCAGGAAGTT ATATGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT 
CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA 
GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT 
AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG 
ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA 
ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT 
CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCTTCATCT 
CCAGTCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAT CAACCTCTGC TGTTAATCAT 
AATGTAAATT TAACTAATGT CAGACTGGAG GAGCCCACCC CAGCTCCTTC CACCTCTTAC 
TCACCACAAG CTGATTCTTT AAGAACACCA AGTACTGAGG CAGCTCACAT AATGCTAAGA 
GATCAAGAAC CATCATTGTC GTCGCATGTA GATCCAACAA AGGTGTATAG AAAGGATCCA 
TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA 
GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG 
AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG 
GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT 
GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT 
AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG 
TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA 
CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA 
CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCCGCAGAG 
TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT 
ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA 
CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC 
CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA 
GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC 
ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT 
CCCAAACCCA CTTCTCAGAA TCAGTTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA 
GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATCG TTCGGTCCAA TCATTATGAC 
CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT 
GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCT 
CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT 
GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT 
CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC 
CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT 
TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA 
CCAAACCGAG AAGATACTGC TCAGCCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA 
GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC 
ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA 
TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGAATTGTC TTCAAAAACT 
CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC 
AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC 
AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA 
GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG 
AGTTCCATAG AAACTGGCGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT 
GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA 
GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC 
CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGCGTCCA TGACTCCTGA CGGTTGGTCT 
TTTGCTCTAA AATCATCCGA CTCCTCGTCG GGTGATCCTA AAACCTGGCA AAACAAGTGT 
CTTCCCGGAG ATCCAAATTA TCTCGTTGGA GCAAACTGTG TTTCTGTCCT TATTGACCAC 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_009428685.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009426960.2 Pan troglodytes tight junction protein 1 (TJP1), transcript variant X2, mRNA.

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CloneID	OPc69375
Clone ID Related Accession (Same CDS sequence)	XM_009428685.2
Accession Version	XM_009428685.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5547bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	tight junction protein ZO-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006482.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009428685.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG 
CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA 
AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC 
AGGGCTCCTG GATTTGGGTT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA 
CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA 
AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT 
GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT 
AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG 
TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CCGCTAAACC TACTAAAGTC 
ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT 
GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT 
GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA 
TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG 
CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC 
GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT 
CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG 
CACTCGCCAC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT 
AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG 
GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT 
GTGTATGCCC AAGTTGGGCA GCCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC 
CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG 
CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT 
CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT 
GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT 
ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG 
TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA 
ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA 
ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT 
ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC 
ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT 
GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT 
CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT 
CGGAAAAGTG CCAGGAAGTT ATATGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT 
CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA 
GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT 
AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG 
ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA 
ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT 
CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCTTCATCT 
CCAGTCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAT CAACCTCTGC TGTTAATCAT 
AATGTAAATT TAACTAATGT CAGACTGGAG GAGCCCACCC CAGCTCCTTC CACCTCTTAC 
TCACCACAAG CTGATTCTTT AAGAACACCA AGTACTGAGG CAGCTCACAT AATGCTAAGA 
GATCAAGAAC CATCATTGTC GTCGCATGTA GATCCAACAA AGGTGTATAG AAAGGATCCA 
TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA 
GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG 
AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG 
GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT 
GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT 
AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG 
TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA 
CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA 
CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCCGCAGAG 
TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT 
ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA 
CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC 
CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA 
GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC 
ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT 
CCCAAACCCA CTTCTCAGAA TCAGTTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA 
GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATCG TTCGGTCCAA TCATTATGAC 
CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT 
GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCT 
CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT 
GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT 
CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC 
CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT 
TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA 
CCAAACCGAG AAGATACTGC TCAGCCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA 
GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC 
ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA 
TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGAATTGTC TTCAAAAACT 
CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC 
AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC 
AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA 
GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG 
AGTTCCATAG AAACTGGCGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT 
GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA 
GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC 
CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGCGTCCA TGACTCCTGA CGGTGATCCT 
AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT 
GTTTCTGTCC TTATTGACCA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009426960.2
CDS	19..5565
Translation	MRRLEGLCLRRRRRRRGPARERSGDKMKYQKYLTVLQMAIGVTPSNRGSLLPLKRKLWVTPSSENPNGATSSVSQGKPSLRRIKGRLHRSKSLDSMDFCELTSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQKAEASSPVPYLSPETNPASSTSAVNHNVNLTNVRLEEPTPAPSTSYSPQADSLRTPSTEAAHIMLRDQEPSLSSHVDPTKVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQPAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPELSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_009428685.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes tight junction protein 1 (TJP1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009428685.2

ORF Insert Sequence:

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG 
CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA 
AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC 
AGGGCTCCTG GATTTGGGTT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA 
CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA 
AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT 
GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT 
AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG 
TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CCGCTAAACC TACTAAAGTC 
ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT 
GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT 
GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA 
TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG 
CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC 
GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT 
CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG 
CACTCGCCAC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT 
AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG 
GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT 
GTGTATGCCC AAGTTGGGCA GCCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC 
CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG 
CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT 
CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT 
GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT 
ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG 
TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA 
ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA 
ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT 
ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC 
ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT 
GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT 
CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT 
CGGAAAAGTG CCAGGAAGTT ATATGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT 
CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA 
GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT 
AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG 
ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA 
ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT 
CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCTTCATCT 
CCAGTCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAT CAACCTCTGC TGTTAATCAT 
AATGTAAATT TAACTAATGT CAGACTGGAG GAGCCCACCC CAGCTCCTTC CACCTCTTAC 
TCACCACAAG CTGATTCTTT AAGAACACCA AGTACTGAGG CAGCTCACAT AATGCTAAGA 
GATCAAGAAC CATCATTGTC GTCGCATGTA GATCCAACAA AGGTGTATAG AAAGGATCCA 
TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA 
GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG 
AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG 
GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT 
GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT 
AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG 
TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA 
CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA 
CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCCGCAGAG 
TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT 
ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA 
CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC 
CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA 
GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC 
ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT 
CCCAAACCCA CTTCTCAGAA TCAGTTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA 
GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATCG TTCGGTCCAA TCATTATGAC 
CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT 
GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCT 
CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT 
GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT 
CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC 
CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT 
TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA 
CCAAACCGAG AAGATACTGC TCAGCCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA 
GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC 
ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA 
TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGAATTGTC TTCAAAAACT 
CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC 
AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC 
AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA 
GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG 
AGTTCCATAG AAACTGGCGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT 
GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA 
GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC 
CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGCGTCCA TGACTCCTGA CGGTGATCCT 
AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT 
GTTTCTGTCC TTATTGACCA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_009428686.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009426961.2 Pan troglodytes tight junction protein 1 (TJP1), transcript variant X3, mRNA.

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CloneID	OPc69376
Clone ID Related Accession (Same CDS sequence)	XM_009428686.2
Accession Version	XM_009428686.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5526bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	tight junction protein ZO-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006482.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009428686.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG 
CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA 
AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC 
AGGGCTCCTG GATTTGGGTT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA 
CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA 
AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT 
GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT 
AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG 
TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CCGCTAAACC TACTAAAGTC 
ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT 
GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT 
GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA 
TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG 
CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC 
GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT 
CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG 
CACTCGCCAC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT 
AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG 
GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT 
GTGTATGCCC AAGTTGGGCA GCCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC 
CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG 
CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT 
CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT 
GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT 
ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG 
TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA 
ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA 
ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT 
ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC 
ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT 
GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT 
CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT 
CGGAAAAGTG CCAGGAAGTT ATATGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT 
CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA 
GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT 
AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG 
ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA 
ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT 
CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCTTCATCT 
CCAGTCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAT CAACCTCTGC TGTTAATCAT 
AATGTAAATT TAACTAATGT CAGACTGGAG GAGCCCACCC CAGCTCCTTC CACCTCTTAC 
TCACCACAAG CTGATTCTTT AAGAACACCA AGTACTGAGG CAGCTCACAT AATGCTAAGA 
GATCAAGAAC CATCATTGTC GTCGCATGTA GATCCAACAA AGGTGTATAG AAAGGATCCA 
TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA 
GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG 
AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG 
GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT 
GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT 
AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG 
TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA 
CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA 
CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCCGCAGAG 
TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT 
ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA 
CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC 
CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA 
GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC 
ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT 
CCCAAACCCA CTTCTCAGAA TCAGTTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA 
GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATCG TTCGGTCCAA TCATTATGAC 
CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT 
GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCT 
CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT 
GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT 
CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC 
CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT 
TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA 
CCAAACCGAG AAGATACTGC TCAGCCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA 
GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC 
ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA 
TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGAATTGTC TTCAAAAACT 
CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC 
AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC 
AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA 
GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG 
AGTTCCATAG AAACTGGCGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT 
GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA 
GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC 
CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGATCCTA AAACCTGGCA AAACAAGTGT 
CTTCCCGGAG ATCCAAATTA TCTCGTTGGA GCAAACTGTG TTTCTGTCCT TATTGACCAC 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009426961.2
CDS	19..5544
Translation	MRRLEGLCLRRRRRRRGPARERSGDKMKYQKYLTVLQMAIGVTPSNRGSLLPLKRKLWVTPSSENPNGATSSVSQGKPSLRRIKGRLHRSKSLDSMDFCELTSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQKAEASSPVPYLSPETNPASSTSAVNHNVNLTNVRLEEPTPAPSTSYSPQADSLRTPSTEAAHIMLRDQEPSLSSHVDPTKVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQPAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPELSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_009428686.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes tight junction protein 1 (TJP1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009428686.2

ORF Insert Sequence:

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG 
CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA 
AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC 
AGGGCTCCTG GATTTGGGTT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA 
CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA 
AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT 
GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT 
AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG 
TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CCGCTAAACC TACTAAAGTC 
ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT 
GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT 
GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA 
TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG 
CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC 
GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT 
CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG 
CACTCGCCAC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT 
AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG 
GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT 
GTGTATGCCC AAGTTGGGCA GCCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC 
CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG 
CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT 
CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT 
GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT 
ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG 
TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA 
ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA 
ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT 
ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC 
ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT 
GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT 
CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT 
CGGAAAAGTG CCAGGAAGTT ATATGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT 
CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA 
GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT 
AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG 
ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA 
ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT 
CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCTTCATCT 
CCAGTCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAT CAACCTCTGC TGTTAATCAT 
AATGTAAATT TAACTAATGT CAGACTGGAG GAGCCCACCC CAGCTCCTTC CACCTCTTAC 
TCACCACAAG CTGATTCTTT AAGAACACCA AGTACTGAGG CAGCTCACAT AATGCTAAGA 
GATCAAGAAC CATCATTGTC GTCGCATGTA GATCCAACAA AGGTGTATAG AAAGGATCCA 
TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA 
GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG 
AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG 
GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT 
GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT 
AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG 
TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA 
CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA 
CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCCGCAGAG 
TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT 
ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA 
CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC 
CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA 
GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC 
ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT 
CCCAAACCCA CTTCTCAGAA TCAGTTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA 
GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATCG TTCGGTCCAA TCATTATGAC 
CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT 
GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCT 
CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT 
GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT 
CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC 
CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT 
TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA 
CCAAACCGAG AAGATACTGC TCAGCCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA 
GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC 
ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA 
TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGAATTGTC TTCAAAAACT 
CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC 
AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC 
AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA 
GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG 
AGTTCCATAG AAACTGGCGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT 
GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA 
GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC 
CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGATCCTA AAACCTGGCA AAACAAGTGT 
CTTCCCGGAG ATCCAAATTA TCTCGTTGGA GCAAACTGTG TTTCTGTCCT TATTGACCAC 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_009428689.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009426964.2 Pan troglodytes tight junction protein 1 (TJP1), transcript variant X4, mRNA.

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CloneID	OPc69377
Clone ID Related Accession (Same CDS sequence)	XM_009428689.2
Accession Version	XM_009428689.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5346bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	tight junction protein ZO-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006482.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009428689.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG 
CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA 
AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC 
AGGGCTCCTG GATTTGGGTT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA 
CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA 
AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT 
GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT 
AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG 
TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CCGCTAAACC TACTAAAGTC 
ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT 
GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT 
GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA 
TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG 
CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC 
GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT 
CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG 
CACTCGCCAC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT 
AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG 
GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT 
GTGTATGCCC AAGTTGGGCA GCCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC 
CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG 
CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT 
CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT 
GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT 
ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG 
TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA 
ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA 
ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT 
ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC 
ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT 
GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT 
CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT 
CGGAAAAGTG CCAGGAAGTT ATATGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT 
CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA 
GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT 
AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG 
ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA 
ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT 
CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGGTGTATAG AAAGGATCCA 
TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA 
GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG 
AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG 
GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT 
GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT 
AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG 
TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA 
CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA 
CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCCGCAGAG 
TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT 
ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA 
CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC 
CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA 
GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC 
ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT 
CCCAAACCCA CTTCTCAGAA TCAGTTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA 
GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATCG TTCGGTCCAA TCATTATGAC 
CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT 
GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCT 
CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT 
GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT 
CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC 
CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT 
TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA 
CCAAACCGAG AAGATACTGC TCAGCCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA 
GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC 
ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA 
TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGAATTGTC TTCAAAAACT 
CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC 
AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC 
AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA 
GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG 
AGTTCCATAG AAACTGGCGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT 
GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA 
GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC 
CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGCGTCCA TGACTCCTGA CGGTTGGTCT 
TTTGCTCTAA AATCATCCGA CTCCTCGTCG GGTGATCCTA AAACCTGGCA AAACAAGTGT 
CTTCCCGGAG ATCCAAATTA TCTCGTTGGA GCAAACTGTG TTTCTGTCCT TATTGACCAC 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009426964.2
CDS	17..5362
Translation	MRRLEGLCLRRRRRRRGPARERSGDKMKYQKYLTVLQMAIGVTPSNRGSLLPLKRKLWVTPSSENPNGATSSVSQGKPSLRRIKGRLHRSKSLDSMDFCELTSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQPAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPELSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_009428689.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes tight junction protein 1 (TJP1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009428689.2

ORF Insert Sequence:

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG 
CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA 
AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC 
AGGGCTCCTG GATTTGGGTT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA 
CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA 
AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT 
GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT 
AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG 
TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CCGCTAAACC TACTAAAGTC 
ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT 
GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT 
GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA 
TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG 
CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC 
GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT 
CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG 
CACTCGCCAC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT 
AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG 
GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT 
GTGTATGCCC AAGTTGGGCA GCCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC 
CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG 
CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT 
CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT 
GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT 
ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG 
TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA 
ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA 
ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT 
ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC 
ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT 
GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT 
CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT 
CGGAAAAGTG CCAGGAAGTT ATATGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT 
CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA 
GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT 
AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG 
ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA 
ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT 
CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGGTGTATAG AAAGGATCCA 
TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA 
GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG 
AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG 
GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT 
GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT 
AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG 
TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA 
CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA 
CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCCGCAGAG 
TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT 
ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA 
CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC 
CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA 
GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC 
ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT 
CCCAAACCCA CTTCTCAGAA TCAGTTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA 
GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATCG TTCGGTCCAA TCATTATGAC 
CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT 
GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCT 
CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT 
GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT 
CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC 
CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT 
TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA 
CCAAACCGAG AAGATACTGC TCAGCCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA 
GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC 
ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA 
TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGAATTGTC TTCAAAAACT 
CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC 
AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC 
AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA 
GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG 
AGTTCCATAG AAACTGGCGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT 
GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA 
GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC 
CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGCGTCCA TGACTCCTGA CGGTTGGTCT 
TTTGCTCTAA AATCATCCGA CTCCTCGTCG GGTGATCCTA AAACCTGGCA AAACAAGTGT 
CTTCCCGGAG ATCCAAATTA TCTCGTTGGA GCAAACTGTG TTTCTGTCCT TATTGACCAC 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_009428690.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009426965.2 Pan troglodytes tight junction protein 1 (TJP1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OPc69382
Clone ID Related Accession (Same CDS sequence)	XM_009428690.2
Accession Version	XM_009428690.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5286bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	tight junction protein ZO-1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006482.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009428690.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG 
CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA 
AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC 
AGGGCTCCTG GATTTGGGTT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA 
CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA 
AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT 
GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT 
AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG 
TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CCGCTAAACC TACTAAAGTC 
ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT 
GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT 
GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA 
TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG 
CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC 
GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT 
CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG 
CACTCGCCAC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT 
AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG 
GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT 
GTGTATGCCC AAGTTGGGCA GCCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC 
CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG 
CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT 
CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT 
GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT 
ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG 
TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA 
ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA 
ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT 
ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC 
ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT 
GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT 
CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT 
CGGAAAAGTG CCAGGAAGTT ATATGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT 
CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA 
GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT 
AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG 
ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA 
ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT 
CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGGTGTATAG AAAGGATCCA 
TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA 
GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG 
AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG 
GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT 
GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT 
AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG 
TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA 
CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA 
CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCCGCAGAG 
TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT 
ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA 
CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC 
CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA 
GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC 
ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT 
CCCAAACCCA CTTCTCAGAA TCAGTTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA 
GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATCG TTCGGTCCAA TCATTATGAC 
CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT 
GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCT 
CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT 
GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT 
CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC 
CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT 
TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA 
CCAAACCGAG AAGATACTGC TCAGCCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA 
GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC 
ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA 
TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGAATTGTC TTCAAAAACT 
CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC 
AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC 
AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA 
GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG 
AGTTCCATAG AAACTGGCGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT 
GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA 
GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC 
CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGATCCTA AAACCTGGCA AAACAAGTGT 
CTTCCCGGAG ATCCAAATTA TCTCGTTGGA GCAAACTGTG TTTCTGTCCT TATTGACCAC 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009426965.2
CDS	18..5303
Translation	MRRLEGLCLRRRRRRRGPARERSGDKMKYQKYLTVLQMAIGVTPSNRGSLLPLKRKLWVTPSSENPNGATSSVSQGKPSLRRIKGRLHRSKSLDSMDFCELTSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQPAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPELSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_009428690.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes tight junction protein 1 (TJP1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009428690.2

ORF Insert Sequence:

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG 
CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA 
AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC 
AGGGCTCCTG GATTTGGGTT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA 
CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA 
AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT 
GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT 
AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG 
TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CCGCTAAACC TACTAAAGTC 
ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT 
GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT 
GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA 
TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG 
CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC 
GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT 
CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG 
CACTCGCCAC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT 
AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG 
GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT 
GTGTATGCCC AAGTTGGGCA GCCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC 
CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG 
CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT 
CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT 
GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT 
ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG 
TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA 
ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA 
ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT 
ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC 
ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT 
GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT 
CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT 
CGGAAAAGTG CCAGGAAGTT ATATGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT 
CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA 
GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT 
AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG 
ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA 
ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT 
CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGGTGTATAG AAAGGATCCA 
TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA 
GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG 
AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG 
GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT 
GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT 
AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG 
TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA 
CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA 
CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCCGCAGAG 
TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT 
ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA 
CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC 
CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA 
GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC 
ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT 
CCCAAACCCA CTTCTCAGAA TCAGTTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA 
GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATCG TTCGGTCCAA TCATTATGAC 
CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT 
GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCT 
CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT 
GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT 
CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC 
CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT 
TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA 
CCAAACCGAG AAGATACTGC TCAGCCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA 
GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC 
ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA 
TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGAATTGTC TTCAAAAACT 
CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC 
AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC 
AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA 
GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG 
AGTTCCATAG AAACTGGCGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT 
GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA 
GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC 
CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGATCCTA AAACCTGGCA AAACAAGTGT 
CTTCCCGGAG ATCCAAATTA TCTCGTTGGA GCAAACTGTG TTTCTGTCCT TATTGACCAC 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	TJP1
Entrez Gene ID	453253
Full Name	tight junction protein 1
General protein information	Preferred Names tight junction protein 1 Names tight junction protein ZO-1 tight junction protein 1 (zona occludens 1)
Gene Type	protein-coding
Organism	Pan troglodytes(chimpanzee)

Homo sapiens (human)	TJP1	NP_003248.3
	TJP1	XP_002804738.1
Canis lupus familiaris (dog)	TJP1	NP_001003140.1
Rattus norvegicus (Norway rat)	Tjp1	NP_001099736.1
Danio rerio (zebrafish)	LOC565459	XP_003201509.2
Bos taurus (cattle)	TJP1	XP_002696696.3
Gallus gallus (chicken)	TJP1	XP_413773.4
Danio rerio (zebrafish)	tjp1a	XP_005168990.1
Pan troglodytes (chimpanzee)	TJP1	XP_001163120.1
Mus musculus (house mouse)	Tjp1	NP_033412.2
Xenopus tropicalis (tropical clawed frog)	tjp1	XP_004917990.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

TJP1 cDNA ORF clone, Pan troglodytes(chimpanzee)

Gene

Clones

gRNAs

Target ORF information:

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ORF Insert Sequence:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

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Target ORF information:

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Target ORF information:

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Target ORF information:

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