Tjp1 gene cDNA ORF clone, Mus musculus(house mouse)

mRNA	Protein	Name
XM_006540779.3	XP_006540842.1	tight junction protein ZO-1 isoform X2
XM_011250843.2	XP_011249145.1	tight junction protein ZO-1 isoform X5
XM_006540783.4	XP_006540846.1	tight junction protein ZO-1 isoform X7
NM_001163574.1	NP_001157046.1	tight junction protein ZO-1 isoform 2
XM_006540781.3	XP_006540844.1	tight junction protein ZO-1 isoform X4
XM_006540781.4	XP_006540844.1	tight junction protein ZO-1 isoform X4
XM_006540782.4	XP_006540845.1	tight junction protein ZO-1 isoform X6
XM_006540784.4	XP_006540847.1	tight junction protein ZO-1 isoform X8
XM_006540786.4	XP_006540849.1	tight junction protein ZO-1 isoform X11
XM_006540778.3	XP_006540841.1	tight junction protein ZO-1 isoform X1
XM_006540785.3	XP_006540848.1	tight junction protein ZO-1 isoform X9
XM_006540786.3	XP_006540849.1	tight junction protein ZO-1 isoform X10
XM_006540779.4	XP_006540842.1	tight junction protein ZO-1 isoform X2
XM_006540780.4	XP_006540843.1	tight junction protein ZO-1 isoform X3
XM_006540783.3	XP_006540846.1	tight junction protein ZO-1 isoform X7
XM_006540778.4	XP_006540841.1	tight junction protein ZO-1 isoform X1
XM_011250843.3	XP_011249145.1	tight junction protein ZO-1 isoform X5
XM_030242356.1	XP_030098216.1	tight junction protein ZO-1 isoform X10
XM_006540780.3	XP_006540843.1	tight junction protein ZO-1 isoform X3
XM_006540782.3	XP_006540845.1	tight junction protein ZO-1 isoform X6
XM_006540784.3	XP_006540847.1	tight junction protein ZO-1 isoform X8
XM_006540785.4	XP_006540848.1	tight junction protein ZO-1 isoform X9
NM_009386.2	NP_033412.2	tight junction protein ZO-1 isoform 1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu38768	XM_006540778.4 Latest version!	Mus musculus tight junction protein 1 (Tjp1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu38769	XM_006540779.4 Latest version!	Mus musculus tight junction protein 1 (Tjp1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu38770	XM_006540780.4 Latest version!	Mus musculus tight junction protein 1 (Tjp1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu38771	XM_006540781.4 Latest version!	Mus musculus tight junction protein 1 (Tjp1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu38772	XM_006540782.4 Latest version!	Mus musculus tight junction protein 1 (Tjp1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu38773	XM_006540783.4 Latest version!	Mus musculus tight junction protein 1 (Tjp1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu38774	XM_006540784.4 Latest version!	Mus musculus tight junction protein 1 (Tjp1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu38775	XM_006540785.4 Latest version!	Mus musculus tight junction protein 1 (Tjp1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu38776	XM_006540786.4 Latest version!	Mus musculus tight junction protein 1 (Tjp1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu68895	XM_011250843.3 Latest version!	Mus musculus tight junction protein 1 (Tjp1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu89424	XM_030242356.1 Latest version!	Mus musculus tight junction protein 1 (Tjp1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu20010	NM_009386.2 Latest version!	Mus musculus tight junction protein 1 (Tjp1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1280.30 $1829.00
OMu22535	NM_001163574.1 Latest version!	Mus musculus tight junction protein 1 (Tjp1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
View Old Accession Versions >>
OMu38771	XM_006540781.3	Mus musculus tight junction protein 1 (Tjp1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OMu38776	XM_006540786.3	Mus musculus tight junction protein 1 (Tjp1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OMu38769	XM_006540779.3	Mus musculus tight junction protein 1 (Tjp1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OMu38773	XM_006540783.3	Mus musculus tight junction protein 1 (Tjp1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OMu38768	XM_006540778.3	Mus musculus tight junction protein 1 (Tjp1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OMu68895	XM_011250843.2	Mus musculus tight junction protein 1 (Tjp1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OMu38770	XM_006540780.3	Mus musculus tight junction protein 1 (Tjp1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OMu38772	XM_006540782.3	Mus musculus tight junction protein 1 (Tjp1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OMu38774	XM_006540784.3	Mus musculus tight junction protein 1 (Tjp1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu38775	XM_006540785.3	Mus musculus tight junction protein 1 (Tjp1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
Hide Old Accession Versions >>

Tjp1 ( XM_006540778.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006540841.1 Mus musculus tight junction protein 1 (Tjp1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu38768
Clone ID Related Accession (Same CDS sequence)	XM_006540778.3 , XM_006540778.4
Accession Version	XM_006540778.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5628bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tight junction protein ZO-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006540778.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT 
CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA 
CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC 
AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA 
CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA 
AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT 
GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG 
AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG 
TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC 
ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT 
GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT 
GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA 
CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC 
TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT 
GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA 
CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG 
CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT 
AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG 
GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT 
GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT 
CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC 
CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT 
CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT 
GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT 
ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG 
TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA 
ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA 
ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT 
ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC 
ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT 
GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC 
CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT 
CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT 
CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA 
GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT 
AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG 
ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA 
ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT 
CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCCTCATCT 
CCAGTCCCTT ACCTTTCGCC TGAAACAACC CCAGCATCAT CAGCCTCTGC AGTTAATCAT 
AATGTCAGTG TAACTAATGT CAGCCTGGAG GAGCCTGCCC CAGCCCCTCC CACCTCGCAC 
GCATCACAGC CTGGTTGTTT AGGAGCACCA AGTGCTGAGG CAGCTCACGT AGTGCTCAGA 
GGTGAAGGAC CACCATTGCC GCCGCATGCA GACCCAGCAA AGGTGTACAG GAAGGAGCCG 
TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA 
GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA 
AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA 
GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT 
GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT 
CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT 
TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG 
CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC 
AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC 
AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC 
TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT 
TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA 
ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC 
AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT 
GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT 
AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG 
CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT 
GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG 
AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT 
TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAAT TTCTTGGCTC TTACACTAGC 
TATGACTACT TGAAAAGGAA CATCAAGTGG GGAAAACCCG AAACTGATGC TGTGGATAGA 
TCATTCAGTG AGAAACGTTA TGATCCAGCC CAGGCCACGC CTCCTCCTCC TCCGTTGCCC 
TCACAGTACA GCCAGCCAGC TCCACCTCTG TCCAGCTCTT CTCTCCACAT ACATTCCAAG 
GGCGCCCAGG GTGAAGGCAA CTCAGTATCA TTGGATTTTC AGAACTCATA TATGTCCAAA 
CCAGACCCAC CCCCATCTCA GAGTAAACCA GCAACTTTCA GACCACCAAC TCGAGAAGAC 
CCCCCTCAGA CTTTCTATCC GCAGAAAAGT TTCCCAGACA AAGCTCCAGT TAACGGAGCT 
GAGCAGACTC AGAAAACCAT CACTCCGGTG TACAATCGAT TCACACCAAA GCCGTACACA 
AGTTCTGCCC GGCCATTTGA ACGCAAATTT GAAAGTCCGA AGTTCAACCA TAATCTTCTG 
CCAAGTGAAA CTGTACATAA ACCTGAATTG TCTTCAAAAA CTCCCACTTC CCCAAAAACT 
CTTATGAAAG CTCATAGTTC AACACAGCCT CCAGAGTTTG ACAGTGGAGT TGAAACTTTC 
TCTGTTCACA CAGATAAGCC TAAATATCAA ATGAATAATA TCAGCACCAT GCCTAAAGCT 
GTCCCTGTGA GTCCTTCAGC TGTGGAAGAA GATGAAGATG AGGATGGTCA TACTGTAGTG 
GCTACAGCTC GTGGCATTTT TAACAGCAAT GGTGGTGTGT TGAGTTCCAT AGAAACCGGT 
GTTAGTATAA TTATCCCACA AGGAGCCATT CCTGAAGGAA TTGAGCAAGA AATCTATTTC 
AAAGTCTGCA GAGACAATAG CATTCTCCCA CCTTTAGATA AAGAGAAAGG TGAAACTCTG 
CTGAGCCCCC TAGTGATGTG TGGGCCCCAT GGCCTCAAGT TCCTGAAGCC CGTGGAGCTA 
CGCTTGCCAC ACTGTGCGTC CATGACTCCT GACGGTTGGT CTTTTGCTCT AAAATCATCC 
GACTCCTCGT CGGGTGACCC TAAAACCTGG CAAAACAAGT GTCTTCCTGG AGATCCGAAT 
TACCTTGTTG GAGCCAACTG TGTTTCTGTC CTGATTGACC ACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006540841.1
CDS	115..5742
Translation	MRRLEGLCLRRRRRRRGPARERSGDKMKYQKYLTVLQMAIGVTPSNRGSLLPLKRKLWVTPSSENPHGATSTVSQGKPSLRRIKGRLHRSKSLDSIDFCELTSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSHPDPEPVSDNEDDSYDEEVHDPRAGRGALANRRSEKSWARDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSQSRSPDQRSEPSDHSTQSPQQPSNGSLRSREEERMSKPGAISTPVKHVDDHPPKAVEEVTVEKNEKQTPTLPEPKPVYAQVGQPDVDLPVSPSDGALPNSAHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDHRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQAIHRIDSPGLKPASQQKAEASSPVPYLSPETTPASSASAVNHNVSVTNVSLEEPAPAPPTSHASQPGCLGAPSAEAAHVVLRGEGPPLPPHADPAKVYRKEPYSEEMMRQNHILKQPALGHPGQRPDKEPNLAYEPQLPYIEKQASRDLEQPSYRYEVSSYTDQFSRNYDHRLRFEDRIPTYEDQWSYYDDKQPYQPRPFENQHPRDLDSRQHPEEASERGYFQRFEEPAPLSYDSRTRYEQLPRTSTLRHEEQPAPAYEVHNRYRPEAQPYSSTGPKSSEPKQYFDQYPRSYEQVPPPGFTSKTGHYEPLHGAAVVPPLIPSSQQKPEVLPSATKPQPPPPTLTEEEEDPAMKPQSVLTRVKMFENKRSASLENKKDVNDTASFKPPEVASKPPGASLAGPKPVPQSQFSEHDKTLYRLPEPQKPQVKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFESKPSAHLPAGHHSEPAKPVHSQSQPNFSSYSSKFLGSYTSYDYLKRNIKWGKPETDAVDRSFSEKRYDPAQATPPPPPLPSQYSQPAPPLSSSSLHIHSKGAQGEGNSVSLDFQNSYMSKPDPPPSQSKPATFRPPTREDPPQTFYPQKSFPDKAPVNGAEQTQKTITPVYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETVHKPELSSKTPTSPKTLMKAHSSTQPPEFDSGVETFSVHTDKPKYQMNNISTMPKAVPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_006540778.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus tight junction protein 1 (Tjp1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006540778.4

ORF Insert Sequence:

ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT 
CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA 
CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC 
AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA 
CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA 
AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT 
GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG 
AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG 
TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC 
ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT 
GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT 
GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA 
CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC 
TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT 
GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA 
CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG 
CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT 
AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG 
GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT 
GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT 
CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC 
CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT 
CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT 
GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT 
ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG 
TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA 
ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA 
ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT 
ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC 
ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT 
GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC 
CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT 
CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT 
CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA 
GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT 
AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG 
ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA 
ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT 
CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCCTCATCT 
CCAGTCCCTT ACCTTTCGCC TGAAACAACC CCAGCATCAT CAGCCTCTGC AGTTAATCAT 
AATGTCAGTG TAACTAATGT CAGCCTGGAG GAGCCTGCCC CAGCCCCTCC CACCTCGCAC 
GCATCACAGC CTGGTTGTTT AGGAGCACCA AGTGCTGAGG CAGCTCACGT AGTGCTCAGA 
GGTGAAGGAC CACCATTGCC GCCGCATGCA GACCCAGCAA AGGTGTACAG GAAGGAGCCG 
TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA 
GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA 
AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA 
GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT 
GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT 
CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT 
TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG 
CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC 
AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC 
AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC 
TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT 
TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA 
ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC 
AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT 
GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT 
AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG 
CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT 
GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG 
AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT 
TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAAT TTCTTGGCTC TTACACTAGC 
TATGACTACT TGAAAAGGAA CATCAAGTGG GGAAAACCCG AAACTGATGC TGTGGATAGA 
TCATTCAGTG AGAAACGTTA TGATCCAGCC CAGGCCACGC CTCCTCCTCC TCCGTTGCCC 
TCACAGTACA GCCAGCCAGC TCCACCTCTG TCCAGCTCTT CTCTCCACAT ACATTCCAAG 
GGCGCCCAGG GTGAAGGCAA CTCAGTATCA TTGGATTTTC AGAACTCATA TATGTCCAAA 
CCAGACCCAC CCCCATCTCA GAGTAAACCA GCAACTTTCA GACCACCAAC TCGAGAAGAC 
CCCCCTCAGA CTTTCTATCC GCAGAAAAGT TTCCCAGACA AAGCTCCAGT TAACGGAGCT 
GAGCAGACTC AGAAAACCAT CACTCCGGTG TACAATCGAT TCACACCAAA GCCGTACACA 
AGTTCTGCCC GGCCATTTGA ACGCAAATTT GAAAGTCCGA AGTTCAACCA TAATCTTCTG 
CCAAGTGAAA CTGTACATAA ACCTGAATTG TCTTCAAAAA CTCCCACTTC CCCAAAAACT 
CTTATGAAAG CTCATAGTTC AACACAGCCT CCAGAGTTTG ACAGTGGAGT TGAAACTTTC 
TCTGTTCACA CAGATAAGCC TAAATATCAA ATGAATAATA TCAGCACCAT GCCTAAAGCT 
GTCCCTGTGA GTCCTTCAGC TGTGGAAGAA GATGAAGATG AGGATGGTCA TACTGTAGTG 
GCTACAGCTC GTGGCATTTT TAACAGCAAT GGTGGTGTGT TGAGTTCCAT AGAAACCGGT 
GTTAGTATAA TTATCCCACA AGGAGCCATT CCTGAAGGAA TTGAGCAAGA AATCTATTTC 
AAAGTCTGCA GAGACAATAG CATTCTCCCA CCTTTAGATA AAGAGAAAGG TGAAACTCTG 
CTGAGCCCCC TAGTGATGTG TGGGCCCCAT GGCCTCAAGT TCCTGAAGCC CGTGGAGCTA 
CGCTTGCCAC ACTGTGCGTC CATGACTCCT GACGGTTGGT CTTTTGCTCT AAAATCATCC 
GACTCCTCGT CGGGTGACCC TAAAACCTGG CAAAACAAGT GTCTTCCTGG AGATCCGAAT 
TACCTTGTTG GAGCCAACTG TGTTTCTGTC CTGATTGACC ACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tjp1 ( XM_006540779.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006540842.1 Mus musculus tight junction protein 1 (Tjp1), transcript variant X2, mRNA.

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CloneID	OMu38769
Clone ID Related Accession (Same CDS sequence)	XM_006540779.3 , XM_006540779.4
Accession Version	XM_006540779.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tight junction protein ZO-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006540779.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT 
CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA 
CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC 
AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA 
CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA 
AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT 
GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG 
AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG 
TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC 
ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT 
GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT 
GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA 
CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC 
TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT 
GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA 
CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG 
CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT 
AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG 
GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT 
GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT 
CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC 
CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT 
CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT 
GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT 
ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG 
TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA 
ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA 
ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT 
ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC 
ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT 
GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC 
CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT 
CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT 
CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA 
GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT 
AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG 
ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA 
ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT 
CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCCTCATCT 
CCAGTCCCTT ACCTTTCGCC TGAAACAACC CCAGCATCAT CAGCCTCTGC AGTTAATCAT 
AATGTCAGTG TAACTAATGT CAGCCTGGAG GAGCCTGCCC CAGCCCCTCC CACCTCGCAC 
GCATCACAGC CTGGTTGTTT AGGAGCACCA AGTGCTGAGG CAGCTCACGT AGTGCTCAGA 
GGTGAAGGAC CACCATTGCC GCCGCATGCA GACCCAGCAA AGGTGTACAG GAAGGAGCCG 
TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA 
GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA 
AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA 
GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT 
GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT 
CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT 
TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG 
CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC 
AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC 
AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC 
TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT 
TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA 
ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC 
AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT 
GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT 
AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG 
CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT 
GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG 
AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT 
TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAAT TTCTTGGCTC TTACACTAGC 
TATGACTACT TGAAAAGGAA CATCAAGTGG GGAAAACCCG AAACTGATGC TGTGGATAGA 
TCATTCAGTG AGAAACGTTA TGATCCAGCC CAGGCCACGC CTCCTCCTCC TCCGTTGCCC 
TCACAGTACA GCCAGCCAGC TCCACCTCTG TCCAGCTCTT CTCTCCACAT ACATTCCAAG 
GGCGCCCAGG GTGAAGGCAA CTCAGTATCA TTGGATTTTC AGAACTCATA TATGTCCAAA 
CCAGACCCAC CCCCATCTCA GAGTAAACCA GCAACTTTCA GACCACCAAC TCGAGAAGAC 
CCCCCTCAGA CTTTCTATCC GCAGAAAAGT TTCCCAGACA AAGCTCCAGT TAACGGAGCT 
GAGCAGACTC AGAAAACCAT CACTCCGGTG TACAATCGAT TCACACCAAA GCCGTACACA 
AGTTCTGCCC GGCCATTTGA ACGCAAATTT GAAAGTCCGA AGTTCAACCA TAATCTTCTG 
CCAAGTGAAA CTGTACATAA ACCTGAATTG TCTTCAAAAA CTCCCACTTC CCCAAAAACT 
CTTATGAAAG CTCATAGTTC AACACAGCCT CCAGAGTTTG ACAGTGGAGT TGAAACTTTC 
TCTGTTCACA CAGATAAGCC TAAATATCAA ATGAATAATA TCAGCACCAT GCCTAAAGCT 
GTCCCTGTGA GTCCTTCAGC TGTGGAAGAA GATGAAGATG AGGATGGTCA TACTGTAGTG 
GCTACAGCTC GTGGCATTTT TAACAGCAAT GGTGGTGTGT TGAGTTCCAT AGAAACCGGT 
GTTAGTATAA TTATCCCACA AGGAGCCATT CCTGAAGGAA TTGAGCAAGA AATCTATTTC 
AAAGTCTGCA GAGACAATAG CATTCTCCCA CCTTTAGATA AAGAGAAAGG TGAAACTCTG 
CTGAGCCCCC TAGTGATGTG TGGGCCCCAT GGCCTCAAGT TCCTGAAGCC CGTGGAGCTA 
CGCTTGCCAC ACTGTGCGTC CATGACTCCT GACGGTGACC CTAAAACCTG GCAAAACAAG 
TGTCTTCCTG GAGATCCGAA TTACCTTGTT GGAGCCAACT GTGTTTCTGT CCTGATTGAC 
CACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006540842.1
CDS	115..5703
Translation	MRRLEGLCLRRRRRRRGPARERSGDKMKYQKYLTVLQMAIGVTPSNRGSLLPLKRKLWVTPSSENPHGATSTVSQGKPSLRRIKGRLHRSKSLDSIDFCELTSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSHPDPEPVSDNEDDSYDEEVHDPRAGRGALANRRSEKSWARDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSQSRSPDQRSEPSDHSTQSPQQPSNGSLRSREEERMSKPGAISTPVKHVDDHPPKAVEEVTVEKNEKQTPTLPEPKPVYAQVGQPDVDLPVSPSDGALPNSAHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDHRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQAIHRIDSPGLKPASQQKAEASSPVPYLSPETTPASSASAVNHNVSVTNVSLEEPAPAPPTSHASQPGCLGAPSAEAAHVVLRGEGPPLPPHADPAKVYRKEPYSEEMMRQNHILKQPALGHPGQRPDKEPNLAYEPQLPYIEKQASRDLEQPSYRYEVSSYTDQFSRNYDHRLRFEDRIPTYEDQWSYYDDKQPYQPRPFENQHPRDLDSRQHPEEASERGYFQRFEEPAPLSYDSRTRYEQLPRTSTLRHEEQPAPAYEVHNRYRPEAQPYSSTGPKSSEPKQYFDQYPRSYEQVPPPGFTSKTGHYEPLHGAAVVPPLIPSSQQKPEVLPSATKPQPPPPTLTEEEEDPAMKPQSVLTRVKMFENKRSASLENKKDVNDTASFKPPEVASKPPGASLAGPKPVPQSQFSEHDKTLYRLPEPQKPQVKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFESKPSAHLPAGHHSEPAKPVHSQSQPNFSSYSSKFLGSYTSYDYLKRNIKWGKPETDAVDRSFSEKRYDPAQATPPPPPLPSQYSQPAPPLSSSSLHIHSKGAQGEGNSVSLDFQNSYMSKPDPPPSQSKPATFRPPTREDPPQTFYPQKSFPDKAPVNGAEQTQKTITPVYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETVHKPELSSKTPTSPKTLMKAHSSTQPPEFDSGVETFSVHTDKPKYQMNNISTMPKAVPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_006540779.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus tight junction protein 1 (Tjp1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006540779.4

ORF Insert Sequence:

ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT 
CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA 
CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC 
AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA 
CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA 
AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT 
GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG 
AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG 
TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC 
ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT 
GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT 
GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA 
CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC 
TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT 
GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA 
CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG 
CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT 
AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG 
GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT 
GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT 
CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC 
CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT 
CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT 
GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT 
ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG 
TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA 
ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA 
ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT 
ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC 
ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT 
GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC 
CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT 
CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT 
CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA 
GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT 
AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG 
ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA 
ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT 
CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCCTCATCT 
CCAGTCCCTT ACCTTTCGCC TGAAACAACC CCAGCATCAT CAGCCTCTGC AGTTAATCAT 
AATGTCAGTG TAACTAATGT CAGCCTGGAG GAGCCTGCCC CAGCCCCTCC CACCTCGCAC 
GCATCACAGC CTGGTTGTTT AGGAGCACCA AGTGCTGAGG CAGCTCACGT AGTGCTCAGA 
GGTGAAGGAC CACCATTGCC GCCGCATGCA GACCCAGCAA AGGTGTACAG GAAGGAGCCG 
TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA 
GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA 
AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA 
GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT 
GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT 
CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT 
TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG 
CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC 
AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC 
AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC 
TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT 
TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA 
ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC 
AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT 
GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT 
AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG 
CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT 
GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG 
AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT 
TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAAT TTCTTGGCTC TTACACTAGC 
TATGACTACT TGAAAAGGAA CATCAAGTGG GGAAAACCCG AAACTGATGC TGTGGATAGA 
TCATTCAGTG AGAAACGTTA TGATCCAGCC CAGGCCACGC CTCCTCCTCC TCCGTTGCCC 
TCACAGTACA GCCAGCCAGC TCCACCTCTG TCCAGCTCTT CTCTCCACAT ACATTCCAAG 
GGCGCCCAGG GTGAAGGCAA CTCAGTATCA TTGGATTTTC AGAACTCATA TATGTCCAAA 
CCAGACCCAC CCCCATCTCA GAGTAAACCA GCAACTTTCA GACCACCAAC TCGAGAAGAC 
CCCCCTCAGA CTTTCTATCC GCAGAAAAGT TTCCCAGACA AAGCTCCAGT TAACGGAGCT 
GAGCAGACTC AGAAAACCAT CACTCCGGTG TACAATCGAT TCACACCAAA GCCGTACACA 
AGTTCTGCCC GGCCATTTGA ACGCAAATTT GAAAGTCCGA AGTTCAACCA TAATCTTCTG 
CCAAGTGAAA CTGTACATAA ACCTGAATTG TCTTCAAAAA CTCCCACTTC CCCAAAAACT 
CTTATGAAAG CTCATAGTTC AACACAGCCT CCAGAGTTTG ACAGTGGAGT TGAAACTTTC 
TCTGTTCACA CAGATAAGCC TAAATATCAA ATGAATAATA TCAGCACCAT GCCTAAAGCT 
GTCCCTGTGA GTCCTTCAGC TGTGGAAGAA GATGAAGATG AGGATGGTCA TACTGTAGTG 
GCTACAGCTC GTGGCATTTT TAACAGCAAT GGTGGTGTGT TGAGTTCCAT AGAAACCGGT 
GTTAGTATAA TTATCCCACA AGGAGCCATT CCTGAAGGAA TTGAGCAAGA AATCTATTTC 
AAAGTCTGCA GAGACAATAG CATTCTCCCA CCTTTAGATA AAGAGAAAGG TGAAACTCTG 
CTGAGCCCCC TAGTGATGTG TGGGCCCCAT GGCCTCAAGT TCCTGAAGCC CGTGGAGCTA 
CGCTTGCCAC ACTGTGCGTC CATGACTCCT GACGGTGACC CTAAAACCTG GCAAAACAAG 
TGTCTTCCTG GAGATCCGAA TTACCTTGTT GGAGCCAACT GTGTTTCTGT CCTGATTGAC 
CACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tjp1 ( XM_006540780.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006540843.1 Mus musculus tight junction protein 1 (Tjp1), transcript variant X3, mRNA.

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CloneID	OMu38770
Clone ID Related Accession (Same CDS sequence)	XM_006540780.4 , XM_006540780.3
Accession Version	XM_006540780.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tight junction protein ZO-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006540780.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT 
CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA 
CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC 
AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA 
CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA 
AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT 
GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG 
AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG 
TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC 
ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT 
GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT 
GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA 
CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC 
TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT 
GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA 
CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG 
CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT 
AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG 
GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT 
GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT 
CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC 
CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT 
CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT 
GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT 
ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG 
TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA 
ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA 
ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT 
ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC 
ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT 
GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC 
CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT 
CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT 
CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA 
GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT 
AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG 
ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA 
ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT 
CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCCTCATCT 
CCAGTCCCTT ACCTTTCGCC TGAAACAACC CCAGCATCAT CAGCCTCTGC AGTTAATCAT 
AATGTCAGTG TAACTAATGT CAGCCTGGAG GAGCCTGCCC CAGCCCCTCC CACCTCGCAC 
GCATCACAGC CTGGTTGTTT AGGAGCACCA AGTGCTGAGG CAGCTCACGT AGTGCTCAGA 
GGTGAAGGAC CACCATTGCC GCCGCATGCA GACCCAGCAA AGGTGTACAG GAAGGAGCCG 
TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA 
GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA 
AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA 
GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT 
GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT 
CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT 
TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG 
CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC 
AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC 
AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC 
TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT 
TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA 
ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC 
AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT 
GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT 
AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG 
CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT 
GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG 
AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT 
TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAGG GAAAACCCGA AACTGATGCT 
GTGGATAGAT CATTCAGTGA GAAACGTTAT GATCCAGCCC AGGCCACGCC TCCTCCTCCT 
CCGTTGCCCT CACAGTACAG CCAGCCAGCT CCACCTCTGT CCAGCTCTTC TCTCCACATA 
CATTCCAAGG GCGCCCAGGG TGAAGGCAAC TCAGTATCAT TGGATTTTCA GAACTCATAT 
ATGTCCAAAC CAGACCCACC CCCATCTCAG AGTAAACCAG CAACTTTCAG ACCACCAACT 
CGAGAAGACC CCCCTCAGAC TTTCTATCCG CAGAAAAGTT TCCCAGACAA AGCTCCAGTT 
AACGGAGCTG AGCAGACTCA GAAAACCATC ACTCCGGTGT ACAATCGATT CACACCAAAG 
CCGTACACAA GTTCTGCCCG GCCATTTGAA CGCAAATTTG AAAGTCCGAA GTTCAACCAT 
AATCTTCTGC CAAGTGAAAC TGTACATAAA CCTGAATTGT CTTCAAAAAC TCCCACTTCC 
CCAAAAACTC TTATGAAAGC TCATAGTTCA ACACAGCCTC CAGAGTTTGA CAGTGGAGTT 
GAAACTTTCT CTGTTCACAC AGATAAGCCT AAATATCAAA TGAATAATAT CAGCACCATG 
CCTAAAGCTG TCCCTGTGAG TCCTTCAGCT GTGGAAGAAG ATGAAGATGA GGATGGTCAT 
ACTGTAGTGG CTACAGCTCG TGGCATTTTT AACAGCAATG GTGGTGTGTT GAGTTCCATA 
GAAACCGGTG TTAGTATAAT TATCCCACAA GGAGCCATTC CTGAAGGAAT TGAGCAAGAA 
ATCTATTTCA AAGTCTGCAG AGACAATAGC ATTCTCCCAC CTTTAGATAA AGAGAAAGGT 
GAAACTCTGC TGAGCCCCCT AGTGATGTGT GGGCCCCATG GCCTCAAGTT CCTGAAGCCC 
GTGGAGCTAC GCTTGCCACA CTGTGCGTCC ATGACTCCTG ACGGTTGGTC TTTTGCTCTA 
AAATCATCCG ACTCCTCGTC GGGTGACCCT AAAACCTGGC AAAACAAGTG TCTTCCTGGA 
GATCCGAATT ACCTTGTTGG AGCCAACTGT GTTTCTGTCC TGATTGACCA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006540843.1
CDS	115..5691
Translation	MRRLEGLCLRRRRRRRGPARERSGDKMKYQKYLTVLQMAIGVTPSNRGSLLPLKRKLWVTPSSENPHGATSTVSQGKPSLRRIKGRLHRSKSLDSIDFCELTSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSHPDPEPVSDNEDDSYDEEVHDPRAGRGALANRRSEKSWARDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSQSRSPDQRSEPSDHSTQSPQQPSNGSLRSREEERMSKPGAISTPVKHVDDHPPKAVEEVTVEKNEKQTPTLPEPKPVYAQVGQPDVDLPVSPSDGALPNSAHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDHRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQAIHRIDSPGLKPASQQKAEASSPVPYLSPETTPASSASAVNHNVSVTNVSLEEPAPAPPTSHASQPGCLGAPSAEAAHVVLRGEGPPLPPHADPAKVYRKEPYSEEMMRQNHILKQPALGHPGQRPDKEPNLAYEPQLPYIEKQASRDLEQPSYRYEVSSYTDQFSRNYDHRLRFEDRIPTYEDQWSYYDDKQPYQPRPFENQHPRDLDSRQHPEEASERGYFQRFEEPAPLSYDSRTRYEQLPRTSTLRHEEQPAPAYEVHNRYRPEAQPYSSTGPKSSEPKQYFDQYPRSYEQVPPPGFTSKTGHYEPLHGAAVVPPLIPSSQQKPEVLPSATKPQPPPPTLTEEEEDPAMKPQSVLTRVKMFENKRSASLENKKDVNDTASFKPPEVASKPPGASLAGPKPVPQSQFSEHDKTLYRLPEPQKPQVKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFESKPSAHLPAGHHSEPAKPVHSQSQPNFSSYSSKGKPETDAVDRSFSEKRYDPAQATPPPPPLPSQYSQPAPPLSSSSLHIHSKGAQGEGNSVSLDFQNSYMSKPDPPPSQSKPATFRPPTREDPPQTFYPQKSFPDKAPVNGAEQTQKTITPVYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETVHKPELSSKTPTSPKTLMKAHSSTQPPEFDSGVETFSVHTDKPKYQMNNISTMPKAVPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_006540780.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus tight junction protein 1 (Tjp1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006540780.4

ORF Insert Sequence:

ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT 
CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA 
CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC 
AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA 
CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA 
AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT 
GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG 
AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG 
TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC 
ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT 
GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT 
GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA 
CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC 
TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT 
GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA 
CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG 
CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT 
AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG 
GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT 
GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT 
CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC 
CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT 
CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT 
GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT 
ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG 
TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA 
ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA 
ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT 
ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC 
ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT 
GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC 
CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT 
CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT 
CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA 
GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT 
AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG 
ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA 
ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT 
CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCCTCATCT 
CCAGTCCCTT ACCTTTCGCC TGAAACAACC CCAGCATCAT CAGCCTCTGC AGTTAATCAT 
AATGTCAGTG TAACTAATGT CAGCCTGGAG GAGCCTGCCC CAGCCCCTCC CACCTCGCAC 
GCATCACAGC CTGGTTGTTT AGGAGCACCA AGTGCTGAGG CAGCTCACGT AGTGCTCAGA 
GGTGAAGGAC CACCATTGCC GCCGCATGCA GACCCAGCAA AGGTGTACAG GAAGGAGCCG 
TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA 
GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA 
AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA 
GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT 
GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT 
CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT 
TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG 
CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC 
AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC 
AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC 
TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT 
TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA 
ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC 
AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT 
GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT 
AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG 
CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT 
GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG 
AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT 
TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAGG GAAAACCCGA AACTGATGCT 
GTGGATAGAT CATTCAGTGA GAAACGTTAT GATCCAGCCC AGGCCACGCC TCCTCCTCCT 
CCGTTGCCCT CACAGTACAG CCAGCCAGCT CCACCTCTGT CCAGCTCTTC TCTCCACATA 
CATTCCAAGG GCGCCCAGGG TGAAGGCAAC TCAGTATCAT TGGATTTTCA GAACTCATAT 
ATGTCCAAAC CAGACCCACC CCCATCTCAG AGTAAACCAG CAACTTTCAG ACCACCAACT 
CGAGAAGACC CCCCTCAGAC TTTCTATCCG CAGAAAAGTT TCCCAGACAA AGCTCCAGTT 
AACGGAGCTG AGCAGACTCA GAAAACCATC ACTCCGGTGT ACAATCGATT CACACCAAAG 
CCGTACACAA GTTCTGCCCG GCCATTTGAA CGCAAATTTG AAAGTCCGAA GTTCAACCAT 
AATCTTCTGC CAAGTGAAAC TGTACATAAA CCTGAATTGT CTTCAAAAAC TCCCACTTCC 
CCAAAAACTC TTATGAAAGC TCATAGTTCA ACACAGCCTC CAGAGTTTGA CAGTGGAGTT 
GAAACTTTCT CTGTTCACAC AGATAAGCCT AAATATCAAA TGAATAATAT CAGCACCATG 
CCTAAAGCTG TCCCTGTGAG TCCTTCAGCT GTGGAAGAAG ATGAAGATGA GGATGGTCAT 
ACTGTAGTGG CTACAGCTCG TGGCATTTTT AACAGCAATG GTGGTGTGTT GAGTTCCATA 
GAAACCGGTG TTAGTATAAT TATCCCACAA GGAGCCATTC CTGAAGGAAT TGAGCAAGAA 
ATCTATTTCA AAGTCTGCAG AGACAATAGC ATTCTCCCAC CTTTAGATAA AGAGAAAGGT 
GAAACTCTGC TGAGCCCCCT AGTGATGTGT GGGCCCCATG GCCTCAAGTT CCTGAAGCCC 
GTGGAGCTAC GCTTGCCACA CTGTGCGTCC ATGACTCCTG ACGGTTGGTC TTTTGCTCTA 
AAATCATCCG ACTCCTCGTC GGGTGACCCT AAAACCTGGC AAAACAAGTG TCTTCCTGGA 
GATCCGAATT ACCTTGTTGG AGCCAACTGT GTTTCTGTCC TGATTGACCA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tjp1 ( XM_006540781.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006540844.1 Mus musculus tight junction protein 1 (Tjp1), transcript variant X4, mRNA.

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CloneID	OMu38771
Clone ID Related Accession (Same CDS sequence)	XM_006540781.3 , XM_006540781.4
Accession Version	XM_006540781.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5568bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tight junction protein ZO-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006540781.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT 
CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA 
CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC 
AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA 
CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA 
AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT 
GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG 
AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG 
TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC 
ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT 
GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT 
GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA 
CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC 
TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT 
GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA 
CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG 
CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT 
AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG 
GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT 
GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT 
CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC 
CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT 
CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT 
GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT 
ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG 
TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA 
ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA 
ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT 
ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC 
ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT 
GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC 
CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT 
CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT 
CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA 
GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT 
AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG 
ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA 
ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT 
CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCCTCATCT 
CCAGTCCCTT ACCTTTCGCC TGAAACAACC CCAGCATCAT CAGCCTCTGC AGTTAATCAT 
AATGTCAGTG TAACTAATGT CAGCCTGGAG GAGCCTGCCC CAGCCCCTCC CACCTCGCAC 
GCATCACAGC CTGGTTGTTT AGGAGCACCA AGTGCTGAGG CAGCTCACGT AGTGCTCAGA 
GGTGAAGGAC CACCATTGCC GCCGCATGCA GACCCAGCAA AGGTGTACAG GAAGGAGCCG 
TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA 
GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA 
AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA 
GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT 
GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT 
CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT 
TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG 
CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC 
AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC 
AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC 
TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT 
TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA 
ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC 
AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT 
GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT 
AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG 
CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT 
GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG 
AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT 
TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAAT TTCTTGGCTC TTACACTAGC 
TATGACTACT TGAAAAGGAA CATCAAGTGG GGAAAACCCG AAACTGATGC TGTGGATAGA 
TCATTCAGTG AGAAACGTTA TGATCCAGCC CAGGCCACGC CTCCTCCTCC TCCGTTGCCC 
TCACAGTACA GCCAGCCAGC TCCACCTCTG TCCAGCTCTT CTCTCCACAT ACATTCCAAG 
GGCGCCCAGG GTGAAGGCAA CTCAGTATCA TTGGATTTTC AGAACTCATA TATGTCCAAA 
CCAGACCCAC CCCCATCTCA GAGTAAACCA GCAACTTTCA GACCACCAAC TCGAGAAGAC 
CCCCCTCAGA CTTTCTATCC GCAGAAAAGT TTCCCAGACA AAGCTCCAGT TAACGGAGCT 
GAGCAGACTC AGAAAACCAT CACTCCGGTG TACAATCGAT TCACACCAAA GCCGTACACA 
AGTTCTGCCC GGCCATTTGA ACGCAAATTT GAAAGTCCGA AGTTCAACCA TAATCTTCTG 
CCAAGTGAAA CTGTACATAA ACCTGAATTG TCTTCAAAAA CTCCCACTTC CCCAAAAACT 
CTTATGAAAG CTCATAGTTC AACACAGCCT CCAGAGTTTG ACAGTGGAGT TGAAACTTTC 
TCTGTTCACA CAGATAAGCC TAAATATCAA ATGAATAATA TCAGCACCAT GCCTAAAGCT 
GTCCCTGTGA GTCCTTCAGC TGTGGAAGAA GATGAAGATG AGGATGGTCA TACTGTAGTG 
GCTACAGCTC GTGGCATTTT TAACAGCAAT GGTGGTGTGT TGAGTTCCAT AGAAACCGGT 
GTTAGTATAA TTATCCCACA AGGAGCCATT CCTGAAGGAA TTGAGCAAGA AATCTATTTC 
AAAGTCTGCA GAGACAATAG CATTCTCCCA CCTTTAGATA AAGAGAAAGG TGAAACTCTG 
CTGAGCCCCC TAGTGATGTG TGGGCCCCAT GGCCTCAAGT TCCTGAAGCC CGTGGAGCTA 
CGCTTGCCAC ACTGTGACCC TAAAACCTGG CAAAACAAGT GTCTTCCTGG AGATCCGAAT 
TACCTTGTTG GAGCCAACTG TGTTTCTGTC CTGATTGACC ACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006540844.1
CDS	115..5682
Translation	MRRLEGLCLRRRRRRRGPARERSGDKMKYQKYLTVLQMAIGVTPSNRGSLLPLKRKLWVTPSSENPHGATSTVSQGKPSLRRIKGRLHRSKSLDSIDFCELTSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSHPDPEPVSDNEDDSYDEEVHDPRAGRGALANRRSEKSWARDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSQSRSPDQRSEPSDHSTQSPQQPSNGSLRSREEERMSKPGAISTPVKHVDDHPPKAVEEVTVEKNEKQTPTLPEPKPVYAQVGQPDVDLPVSPSDGALPNSAHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDHRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQAIHRIDSPGLKPASQQKAEASSPVPYLSPETTPASSASAVNHNVSVTNVSLEEPAPAPPTSHASQPGCLGAPSAEAAHVVLRGEGPPLPPHADPAKVYRKEPYSEEMMRQNHILKQPALGHPGQRPDKEPNLAYEPQLPYIEKQASRDLEQPSYRYEVSSYTDQFSRNYDHRLRFEDRIPTYEDQWSYYDDKQPYQPRPFENQHPRDLDSRQHPEEASERGYFQRFEEPAPLSYDSRTRYEQLPRTSTLRHEEQPAPAYEVHNRYRPEAQPYSSTGPKSSEPKQYFDQYPRSYEQVPPPGFTSKTGHYEPLHGAAVVPPLIPSSQQKPEVLPSATKPQPPPPTLTEEEEDPAMKPQSVLTRVKMFENKRSASLENKKDVNDTASFKPPEVASKPPGASLAGPKPVPQSQFSEHDKTLYRLPEPQKPQVKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFESKPSAHLPAGHHSEPAKPVHSQSQPNFSSYSSKFLGSYTSYDYLKRNIKWGKPETDAVDRSFSEKRYDPAQATPPPPPLPSQYSQPAPPLSSSSLHIHSKGAQGEGNSVSLDFQNSYMSKPDPPPSQSKPATFRPPTREDPPQTFYPQKSFPDKAPVNGAEQTQKTITPVYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETVHKPELSSKTPTSPKTLMKAHSSTQPPEFDSGVETFSVHTDKPKYQMNNISTMPKAVPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_006540781.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus tight junction protein 1 (Tjp1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006540781.4

ORF Insert Sequence:

ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT 
CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA 
CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC 
AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA 
CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA 
AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT 
GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG 
AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG 
TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC 
ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT 
GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT 
GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA 
CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC 
TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT 
GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA 
CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG 
CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT 
AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG 
GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT 
GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT 
CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC 
CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT 
CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT 
GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT 
ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG 
TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA 
ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA 
ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT 
ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC 
ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT 
GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC 
CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT 
CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT 
CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA 
GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT 
AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG 
ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA 
ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT 
CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCCTCATCT 
CCAGTCCCTT ACCTTTCGCC TGAAACAACC CCAGCATCAT CAGCCTCTGC AGTTAATCAT 
AATGTCAGTG TAACTAATGT CAGCCTGGAG GAGCCTGCCC CAGCCCCTCC CACCTCGCAC 
GCATCACAGC CTGGTTGTTT AGGAGCACCA AGTGCTGAGG CAGCTCACGT AGTGCTCAGA 
GGTGAAGGAC CACCATTGCC GCCGCATGCA GACCCAGCAA AGGTGTACAG GAAGGAGCCG 
TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA 
GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA 
AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA 
GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT 
GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT 
CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT 
TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG 
CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC 
AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC 
AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC 
TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT 
TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA 
ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC 
AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT 
GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT 
AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG 
CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT 
GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG 
AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT 
TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAAT TTCTTGGCTC TTACACTAGC 
TATGACTACT TGAAAAGGAA CATCAAGTGG GGAAAACCCG AAACTGATGC TGTGGATAGA 
TCATTCAGTG AGAAACGTTA TGATCCAGCC CAGGCCACGC CTCCTCCTCC TCCGTTGCCC 
TCACAGTACA GCCAGCCAGC TCCACCTCTG TCCAGCTCTT CTCTCCACAT ACATTCCAAG 
GGCGCCCAGG GTGAAGGCAA CTCAGTATCA TTGGATTTTC AGAACTCATA TATGTCCAAA 
CCAGACCCAC CCCCATCTCA GAGTAAACCA GCAACTTTCA GACCACCAAC TCGAGAAGAC 
CCCCCTCAGA CTTTCTATCC GCAGAAAAGT TTCCCAGACA AAGCTCCAGT TAACGGAGCT 
GAGCAGACTC AGAAAACCAT CACTCCGGTG TACAATCGAT TCACACCAAA GCCGTACACA 
AGTTCTGCCC GGCCATTTGA ACGCAAATTT GAAAGTCCGA AGTTCAACCA TAATCTTCTG 
CCAAGTGAAA CTGTACATAA ACCTGAATTG TCTTCAAAAA CTCCCACTTC CCCAAAAACT 
CTTATGAAAG CTCATAGTTC AACACAGCCT CCAGAGTTTG ACAGTGGAGT TGAAACTTTC 
TCTGTTCACA CAGATAAGCC TAAATATCAA ATGAATAATA TCAGCACCAT GCCTAAAGCT 
GTCCCTGTGA GTCCTTCAGC TGTGGAAGAA GATGAAGATG AGGATGGTCA TACTGTAGTG 
GCTACAGCTC GTGGCATTTT TAACAGCAAT GGTGGTGTGT TGAGTTCCAT AGAAACCGGT 
GTTAGTATAA TTATCCCACA AGGAGCCATT CCTGAAGGAA TTGAGCAAGA AATCTATTTC 
AAAGTCTGCA GAGACAATAG CATTCTCCCA CCTTTAGATA AAGAGAAAGG TGAAACTCTG 
CTGAGCCCCC TAGTGATGTG TGGGCCCCAT GGCCTCAAGT TCCTGAAGCC CGTGGAGCTA 
CGCTTGCCAC ACTGTGACCC TAAAACCTGG CAAAACAAGT GTCTTCCTGG AGATCCGAAT 
TACCTTGTTG GAGCCAACTG TGTTTCTGTC CTGATTGACC ACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tjp1 ( XM_006540782.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006540845.1 Mus musculus tight junction protein 1 (Tjp1), transcript variant X6, mRNA.

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CloneID	OMu38772
Clone ID Related Accession (Same CDS sequence)	XM_006540782.4 , XM_006540782.3
Accession Version	XM_006540782.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5517bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tight junction protein ZO-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006540782.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT 
CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA 
CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC 
AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA 
CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA 
AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT 
GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG 
AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG 
TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC 
ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT 
GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT 
GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA 
CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC 
TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT 
GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA 
CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG 
CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT 
AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG 
GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT 
GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT 
CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC 
CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT 
CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT 
GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT 
ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG 
TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA 
ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA 
ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT 
ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC 
ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT 
GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC 
CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT 
CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT 
CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA 
GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT 
AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG 
ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA 
ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT 
CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCCTCATCT 
CCAGTCCCTT ACCTTTCGCC TGAAACAACC CCAGCATCAT CAGCCTCTGC AGTTAATCAT 
AATGTCAGTG TAACTAATGT CAGCCTGGAG GAGCCTGCCC CAGCCCCTCC CACCTCGCAC 
GCATCACAGC CTGGTTGTTT AGGAGCACCA AGTGCTGAGG CAGCTCACGT AGTGCTCAGA 
GGTGAAGGAC CACCATTGCC GCCGCATGCA GACCCAGCAA AGGTGTACAG GAAGGAGCCG 
TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA 
GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA 
AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA 
GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT 
GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT 
CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT 
TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG 
CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC 
AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC 
AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC 
TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT 
TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA 
ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC 
AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT 
GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT 
AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG 
CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT 
GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG 
AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT 
TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAGG GAAAACCCGA AACTGATGCT 
GTGGATAGAT CATTCAGTGA GAAACGTTAT GATCCAGCCC AGGCCACGCC TCCTCCTCCT 
CCGTTGCCCT CACAGTACAG CCAGCCAGCT CCACCTCTGT CCAGCTCTTC TCTCCACATA 
CATTCCAAGG GCGCCCAGGG TGAAGGCAAC TCAGTATCAT TGGATTTTCA GAACTCATAT 
ATGTCCAAAC CAGACCCACC CCCATCTCAG AGTAAACCAG CAACTTTCAG ACCACCAACT 
CGAGAAGACC CCCCTCAGAC TTTCTATCCG CAGAAAAGTT TCCCAGACAA AGCTCCAGTT 
AACGGAGCTG AGCAGACTCA GAAAACCATC ACTCCGGTGT ACAATCGATT CACACCAAAG 
CCGTACACAA GTTCTGCCCG GCCATTTGAA CGCAAATTTG AAAGTCCGAA GTTCAACCAT 
AATCTTCTGC CAAGTGAAAC TGTACATAAA CCTGAATTGT CTTCAAAAAC TCCCACTTCC 
CCAAAAACTC TTATGAAAGC TCATAGTTCA ACACAGCCTC CAGAGTTTGA CAGTGGAGTT 
GAAACTTTCT CTGTTCACAC AGATAAGCCT AAATATCAAA TGAATAATAT CAGCACCATG 
CCTAAAGCTG TCCCTGTGAG TCCTTCAGCT GTGGAAGAAG ATGAAGATGA GGATGGTCAT 
ACTGTAGTGG CTACAGCTCG TGGCATTTTT AACAGCAATG GTGGTGTGTT GAGTTCCATA 
GAAACCGGTG TTAGTATAAT TATCCCACAA GGAGCCATTC CTGAAGGAAT TGAGCAAGAA 
ATCTATTTCA AAGTCTGCAG AGACAATAGC ATTCTCCCAC CTTTAGATAA AGAGAAAGGT 
GAAACTCTGC TGAGCCCCCT AGTGATGTGT GGGCCCCATG GCCTCAAGTT CCTGAAGCCC 
GTGGAGCTAC GCTTGCCACA CTGTGACCCT AAAACCTGGC AAAACAAGTG TCTTCCTGGA 
GATCCGAATT ACCTTGTTGG AGCCAACTGT GTTTCTGTCC TGATTGACCA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006540845.1
CDS	115..5631
Translation	MRRLEGLCLRRRRRRRGPARERSGDKMKYQKYLTVLQMAIGVTPSNRGSLLPLKRKLWVTPSSENPHGATSTVSQGKPSLRRIKGRLHRSKSLDSIDFCELTSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSHPDPEPVSDNEDDSYDEEVHDPRAGRGALANRRSEKSWARDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSQSRSPDQRSEPSDHSTQSPQQPSNGSLRSREEERMSKPGAISTPVKHVDDHPPKAVEEVTVEKNEKQTPTLPEPKPVYAQVGQPDVDLPVSPSDGALPNSAHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDHRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQAIHRIDSPGLKPASQQKAEASSPVPYLSPETTPASSASAVNHNVSVTNVSLEEPAPAPPTSHASQPGCLGAPSAEAAHVVLRGEGPPLPPHADPAKVYRKEPYSEEMMRQNHILKQPALGHPGQRPDKEPNLAYEPQLPYIEKQASRDLEQPSYRYEVSSYTDQFSRNYDHRLRFEDRIPTYEDQWSYYDDKQPYQPRPFENQHPRDLDSRQHPEEASERGYFQRFEEPAPLSYDSRTRYEQLPRTSTLRHEEQPAPAYEVHNRYRPEAQPYSSTGPKSSEPKQYFDQYPRSYEQVPPPGFTSKTGHYEPLHGAAVVPPLIPSSQQKPEVLPSATKPQPPPPTLTEEEEDPAMKPQSVLTRVKMFENKRSASLENKKDVNDTASFKPPEVASKPPGASLAGPKPVPQSQFSEHDKTLYRLPEPQKPQVKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFESKPSAHLPAGHHSEPAKPVHSQSQPNFSSYSSKGKPETDAVDRSFSEKRYDPAQATPPPPPLPSQYSQPAPPLSSSSLHIHSKGAQGEGNSVSLDFQNSYMSKPDPPPSQSKPATFRPPTREDPPQTFYPQKSFPDKAPVNGAEQTQKTITPVYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETVHKPELSSKTPTSPKTLMKAHSSTQPPEFDSGVETFSVHTDKPKYQMNNISTMPKAVPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_006540782.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus tight junction protein 1 (Tjp1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006540782.4

ORF Insert Sequence:

ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT 
CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA 
CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC 
AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA 
CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA 
AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT 
GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG 
AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG 
TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC 
ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT 
GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT 
GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA 
CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC 
TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT 
GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA 
CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG 
CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT 
AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG 
GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT 
GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT 
CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC 
CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT 
CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT 
GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT 
ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG 
TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA 
ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA 
ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT 
ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC 
ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT 
GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC 
CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT 
CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT 
CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA 
GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT 
AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG 
ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA 
ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT 
CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCCTCATCT 
CCAGTCCCTT ACCTTTCGCC TGAAACAACC CCAGCATCAT CAGCCTCTGC AGTTAATCAT 
AATGTCAGTG TAACTAATGT CAGCCTGGAG GAGCCTGCCC CAGCCCCTCC CACCTCGCAC 
GCATCACAGC CTGGTTGTTT AGGAGCACCA AGTGCTGAGG CAGCTCACGT AGTGCTCAGA 
GGTGAAGGAC CACCATTGCC GCCGCATGCA GACCCAGCAA AGGTGTACAG GAAGGAGCCG 
TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA 
GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA 
AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA 
GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT 
GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT 
CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT 
TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG 
CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC 
AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC 
AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC 
TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT 
TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA 
ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC 
AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT 
GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT 
AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG 
CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT 
GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG 
AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT 
TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAGG GAAAACCCGA AACTGATGCT 
GTGGATAGAT CATTCAGTGA GAAACGTTAT GATCCAGCCC AGGCCACGCC TCCTCCTCCT 
CCGTTGCCCT CACAGTACAG CCAGCCAGCT CCACCTCTGT CCAGCTCTTC TCTCCACATA 
CATTCCAAGG GCGCCCAGGG TGAAGGCAAC TCAGTATCAT TGGATTTTCA GAACTCATAT 
ATGTCCAAAC CAGACCCACC CCCATCTCAG AGTAAACCAG CAACTTTCAG ACCACCAACT 
CGAGAAGACC CCCCTCAGAC TTTCTATCCG CAGAAAAGTT TCCCAGACAA AGCTCCAGTT 
AACGGAGCTG AGCAGACTCA GAAAACCATC ACTCCGGTGT ACAATCGATT CACACCAAAG 
CCGTACACAA GTTCTGCCCG GCCATTTGAA CGCAAATTTG AAAGTCCGAA GTTCAACCAT 
AATCTTCTGC CAAGTGAAAC TGTACATAAA CCTGAATTGT CTTCAAAAAC TCCCACTTCC 
CCAAAAACTC TTATGAAAGC TCATAGTTCA ACACAGCCTC CAGAGTTTGA CAGTGGAGTT 
GAAACTTTCT CTGTTCACAC AGATAAGCCT AAATATCAAA TGAATAATAT CAGCACCATG 
CCTAAAGCTG TCCCTGTGAG TCCTTCAGCT GTGGAAGAAG ATGAAGATGA GGATGGTCAT 
ACTGTAGTGG CTACAGCTCG TGGCATTTTT AACAGCAATG GTGGTGTGTT GAGTTCCATA 
GAAACCGGTG TTAGTATAAT TATCCCACAA GGAGCCATTC CTGAAGGAAT TGAGCAAGAA 
ATCTATTTCA AAGTCTGCAG AGACAATAGC ATTCTCCCAC CTTTAGATAA AGAGAAAGGT 
GAAACTCTGC TGAGCCCCCT AGTGATGTGT GGGCCCCATG GCCTCAAGTT CCTGAAGCCC 
GTGGAGCTAC GCTTGCCACA CTGTGACCCT AAAACCTGGC AAAACAAGTG TCTTCCTGGA 
GATCCGAATT ACCTTGTTGG AGCCAACTGT GTTTCTGTCC TGATTGACCA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tjp1 ( XM_006540783.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006540846.1 Mus musculus tight junction protein 1 (Tjp1), transcript variant X7, mRNA.

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CloneID	OMu38773
Clone ID Related Accession (Same CDS sequence)	XM_006540783.4 , XM_006540783.3
Accession Version	XM_006540783.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5388bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tight junction protein ZO-1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006540783.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT 
CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA 
CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC 
AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA 
CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA 
AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT 
GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG 
AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG 
TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC 
ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT 
GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT 
GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA 
CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC 
TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT 
GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA 
CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG 
CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT 
AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG 
GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT 
GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT 
CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC 
CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT 
CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT 
GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT 
ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG 
TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA 
ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA 
ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT 
ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC 
ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT 
GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC 
CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT 
CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT 
CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA 
GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT 
AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG 
ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA 
ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT 
CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGGTGTACAG GAAGGAGCCG 
TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA 
GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA 
AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA 
GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT 
GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT 
CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT 
TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG 
CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC 
AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC 
AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC 
TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT 
TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA 
ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC 
AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT 
GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT 
AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG 
CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT 
GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG 
AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT 
TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAAT TTCTTGGCTC TTACACTAGC 
TATGACTACT TGAAAAGGAA CATCAAGTGG GGAAAACCCG AAACTGATGC TGTGGATAGA 
TCATTCAGTG AGAAACGTTA TGATCCAGCC CAGGCCACGC CTCCTCCTCC TCCGTTGCCC 
TCACAGTACA GCCAGCCAGC TCCACCTCTG TCCAGCTCTT CTCTCCACAT ACATTCCAAG 
GGCGCCCAGG GTGAAGGCAA CTCAGTATCA TTGGATTTTC AGAACTCATA TATGTCCAAA 
CCAGACCCAC CCCCATCTCA GAGTAAACCA GCAACTTTCA GACCACCAAC TCGAGAAGAC 
CCCCCTCAGA CTTTCTATCC GCAGAAAAGT TTCCCAGACA AAGCTCCAGT TAACGGAGCT 
GAGCAGACTC AGAAAACCAT CACTCCGGTG TACAATCGAT TCACACCAAA GCCGTACACA 
AGTTCTGCCC GGCCATTTGA ACGCAAATTT GAAAGTCCGA AGTTCAACCA TAATCTTCTG 
CCAAGTGAAA CTGTACATAA ACCTGAATTG TCTTCAAAAA CTCCCACTTC CCCAAAAACT 
CTTATGAAAG CTCATAGTTC AACACAGCCT CCAGAGTTTG ACAGTGGAGT TGAAACTTTC 
TCTGTTCACA CAGATAAGCC TAAATATCAA ATGAATAATA TCAGCACCAT GCCTAAAGCT 
GTCCCTGTGA GTCCTTCAGC TGTGGAAGAA GATGAAGATG AGGATGGTCA TACTGTAGTG 
GCTACAGCTC GTGGCATTTT TAACAGCAAT GGTGGTGTGT TGAGTTCCAT AGAAACCGGT 
GTTAGTATAA TTATCCCACA AGGAGCCATT CCTGAAGGAA TTGAGCAAGA AATCTATTTC 
AAAGTCTGCA GAGACAATAG CATTCTCCCA CCTTTAGATA AAGAGAAAGG TGAAACTCTG 
CTGAGCCCCC TAGTGATGTG TGGGCCCCAT GGCCTCAAGT TCCTGAAGCC CGTGGAGCTA 
CGCTTGCCAC ACTGTGCGTC CATGACTCCT GACGGTTGGT CTTTTGCTCT AAAATCATCC 
GACTCCTCGT CGGGTGACCC TAAAACCTGG CAAAACAAGT GTCTTCCTGG AGATCCGAAT 
TACCTTGTTG GAGCCAACTG TGTTTCTGTC CTGATTGACC ACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006540846.1
CDS	115..5502
Translation	MRRLEGLCLRRRRRRRGPARERSGDKMKYQKYLTVLQMAIGVTPSNRGSLLPLKRKLWVTPSSENPHGATSTVSQGKPSLRRIKGRLHRSKSLDSIDFCELTSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSHPDPEPVSDNEDDSYDEEVHDPRAGRGALANRRSEKSWARDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSQSRSPDQRSEPSDHSTQSPQQPSNGSLRSREEERMSKPGAISTPVKHVDDHPPKAVEEVTVEKNEKQTPTLPEPKPVYAQVGQPDVDLPVSPSDGALPNSAHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDHRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQAIHRIDSPGLKPASQQVYRKEPYSEEMMRQNHILKQPALGHPGQRPDKEPNLAYEPQLPYIEKQASRDLEQPSYRYEVSSYTDQFSRNYDHRLRFEDRIPTYEDQWSYYDDKQPYQPRPFENQHPRDLDSRQHPEEASERGYFQRFEEPAPLSYDSRTRYEQLPRTSTLRHEEQPAPAYEVHNRYRPEAQPYSSTGPKSSEPKQYFDQYPRSYEQVPPPGFTSKTGHYEPLHGAAVVPPLIPSSQQKPEVLPSATKPQPPPPTLTEEEEDPAMKPQSVLTRVKMFENKRSASLENKKDVNDTASFKPPEVASKPPGASLAGPKPVPQSQFSEHDKTLYRLPEPQKPQVKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFESKPSAHLPAGHHSEPAKPVHSQSQPNFSSYSSKFLGSYTSYDYLKRNIKWGKPETDAVDRSFSEKRYDPAQATPPPPPLPSQYSQPAPPLSSSSLHIHSKGAQGEGNSVSLDFQNSYMSKPDPPPSQSKPATFRPPTREDPPQTFYPQKSFPDKAPVNGAEQTQKTITPVYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETVHKPELSSKTPTSPKTLMKAHSSTQPPEFDSGVETFSVHTDKPKYQMNNISTMPKAVPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_006540783.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus tight junction protein 1 (Tjp1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006540783.4

ORF Insert Sequence:

ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT 
CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA 
CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC 
AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA 
CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA 
AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT 
GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG 
AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG 
TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC 
ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT 
GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT 
GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA 
CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC 
TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT 
GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA 
CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG 
CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT 
AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG 
GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT 
GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT 
CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC 
CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT 
CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT 
GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT 
ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG 
TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA 
ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA 
ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT 
ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC 
ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT 
GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC 
CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT 
CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT 
CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA 
GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT 
AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG 
ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA 
ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT 
CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGGTGTACAG GAAGGAGCCG 
TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA 
GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA 
AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA 
GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT 
GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT 
CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT 
TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG 
CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC 
AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC 
AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC 
TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT 
TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA 
ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC 
AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT 
GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT 
AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG 
CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT 
GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG 
AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT 
TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAAT TTCTTGGCTC TTACACTAGC 
TATGACTACT TGAAAAGGAA CATCAAGTGG GGAAAACCCG AAACTGATGC TGTGGATAGA 
TCATTCAGTG AGAAACGTTA TGATCCAGCC CAGGCCACGC CTCCTCCTCC TCCGTTGCCC 
TCACAGTACA GCCAGCCAGC TCCACCTCTG TCCAGCTCTT CTCTCCACAT ACATTCCAAG 
GGCGCCCAGG GTGAAGGCAA CTCAGTATCA TTGGATTTTC AGAACTCATA TATGTCCAAA 
CCAGACCCAC CCCCATCTCA GAGTAAACCA GCAACTTTCA GACCACCAAC TCGAGAAGAC 
CCCCCTCAGA CTTTCTATCC GCAGAAAAGT TTCCCAGACA AAGCTCCAGT TAACGGAGCT 
GAGCAGACTC AGAAAACCAT CACTCCGGTG TACAATCGAT TCACACCAAA GCCGTACACA 
AGTTCTGCCC GGCCATTTGA ACGCAAATTT GAAAGTCCGA AGTTCAACCA TAATCTTCTG 
CCAAGTGAAA CTGTACATAA ACCTGAATTG TCTTCAAAAA CTCCCACTTC CCCAAAAACT 
CTTATGAAAG CTCATAGTTC AACACAGCCT CCAGAGTTTG ACAGTGGAGT TGAAACTTTC 
TCTGTTCACA CAGATAAGCC TAAATATCAA ATGAATAATA TCAGCACCAT GCCTAAAGCT 
GTCCCTGTGA GTCCTTCAGC TGTGGAAGAA GATGAAGATG AGGATGGTCA TACTGTAGTG 
GCTACAGCTC GTGGCATTTT TAACAGCAAT GGTGGTGTGT TGAGTTCCAT AGAAACCGGT 
GTTAGTATAA TTATCCCACA AGGAGCCATT CCTGAAGGAA TTGAGCAAGA AATCTATTTC 
AAAGTCTGCA GAGACAATAG CATTCTCCCA CCTTTAGATA AAGAGAAAGG TGAAACTCTG 
CTGAGCCCCC TAGTGATGTG TGGGCCCCAT GGCCTCAAGT TCCTGAAGCC CGTGGAGCTA 
CGCTTGCCAC ACTGTGCGTC CATGACTCCT GACGGTTGGT CTTTTGCTCT AAAATCATCC 
GACTCCTCGT CGGGTGACCC TAAAACCTGG CAAAACAAGT GTCTTCCTGG AGATCCGAAT 
TACCTTGTTG GAGCCAACTG TGTTTCTGTC CTGATTGACC ACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tjp1 ( XM_006540784.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006540847.1 Mus musculus tight junction protein 1 (Tjp1), transcript variant X8, mRNA.

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CloneID	OMu38774
Clone ID Related Accession (Same CDS sequence)	XM_006540784.4 , XM_006540784.3
Accession Version	XM_006540784.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5349bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tight junction protein ZO-1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006540784.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGCCA GGGCCGCGGC CGCTAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAGCACACAG TGACGCTTCA CAGGGCTCCT GGGTTTGGAT TTGGAATTGC AATCTCTGGT 
GGAAGAGATA ATCCTCATTT TCAGAGTGGG GAAACCTCCA TAGTGATTTC TGATGTGTTA 
AAAGGAGGGC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA 
GTTTCAATGG ATAACGTTGA ACATGCTTTT GCTGTTCAGC AGCTAAGGAA GAGTGGGAAA 
AACGCAAAAA TTACTATCCG AAGGAAGAAG AAAGTTCAGA TCCCTGTAAG TCACCCAGAT 
CCTGAGCCGG TGTCTGATAA TGAAGACGAT AGTTATGACG AAGAAGTGCA TGACCCAAGA 
GCTGGCCGCG GTGCTTTAGC GAACAGAAGG AGCGAGAAGA GCTGGGCAAG GGATAGGAGT 
GCAAGCAGGG AGAGGAGCCT GTCCCCTCGC TCGGACAGGC GGTCCGTGGC CTCCAGTCAG 
CCCGCAAAGC CCACCAAGGT CACACTGGTG AAGTCTCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCCA GCCATATATT TGTAAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGTAACATTC AAGAAGGGGA TGTTGTCTTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAAAC ACTGATAGAA AGGTCTAAAG GCAAGTTAAA AATGGTAGTG 
CAAAGAGATG AGCGGGCTAC CTTACTGAAC GTCCCTGACC TTTCGGATAG TATCCATTCT 
GCTAATGCCT CGGAAAGAGA TGACATTTCA GAAATTCAGT CACTAGCATC AGACCATTCA 
GGTCGCTCGC ATGACAGGCC ACCCCGCCGC AGCCAGTCAC GATCTCCTGA CCAACGTTCA 
GAGCCCTCCG ATCATTCCAC GCAGTCTCCA CAGCAGCCCA GCAATGGCAG TCTCCGGAGC 
AGAGAGGAAG AGCGAATGTC TAAACCTGGG GCCATCTCAA CTCCTGTAAA ACATGTAGAC 
GATCATCCAC CCAAAGCAGT GGAAGAAGTT ACAGTTGAGA AAAATGAGAA GCAGACGCCC 
ACTCTTCCAG AACCGAAACC TGTGTATGCT CAAGTTGGAC AACCAGATGT GGATTTACCC 
GTCAGCCCTT CTGATGGTGC TCTGCCTAAT TCAGCTCATG AAGACGGGAT ACTTAGGCCC 
AGCATGAAAC TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGACT AGCTGGTGGA 
AATGATGTCG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAAGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTG AACAATGTAG ATTTCACAAA TATCATAAGG 
GAAGAGGCCG TCCTTTTCCT CCTTGACCTC CCTAAAGGTG AAGAAGTGAC CATACTGGCT 
CAGAAGAAGA AGGACGTTTA TCGCCGCATT GTAGAATCAG ATGTAGGAGA TTCATTCTAT 
ATTAGAACGC ATTTTGAATA TGAAAAAGAA TCTCCTTACG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GGGTCGTGGA TACTTTATAC AATGGAAAGC TGGGCTCTTG GCTTGCCATT 
CGAATTGGCA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAACAGTTAG CCAGTGTACA GTACACACTT CCAAAGACAG CGGGTGGTGA TCGGGCAGAC 
TTCTGGAGGT TTCGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAC 
TTGTCAGCTC AGCCAGTTCA AACTAAGTTC CCAGCTTATG AAAGGGTTGT TCTTCGAGAA 
GCTGGATTCC TAAGACCTGT AACCATCTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
TTGGCAAGAG AGGAGCCAGA TATCTATCAG ATTGCAAAAA GTGAACCACG AGATGCTGGG 
ACTGACCATC GTAGCTCTGG CATCATTCGC CTTCATACAA TAAAGCAAAT CATAGATCAG 
GATAAACATG CTTTATTAGA TGTAACGCCA AATGCGGTTG ATCGTCTTAA TTATGCGCAG 
TGGTATCCAA TTGTTGTGTT CCTTAACCCT GACTCTAAGC AAGGTGTAAA AACAATGAGG 
ATGAGGTTGT GTCCGGAGTC TCGGAAAAGC GCCAGGAAGC TATATGAACG CTCTCATAAG 
CTTCGTAAGA ACAATCACCA TCTCTTCACA ACTACAATTA ACTTAAACTC AATGAATGAT 
GGTTGGTACG GTGCCCTGAA AGAAGCGATT CAGCAGCAAC AGAACCAGCT GGTGTGGGTC 
TCTGAGGGGA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA CGATCGTCTG 
TCCTACCTGT CAGCCCCAGG TAGTGAGTAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA TACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTGGG GACTCCCCCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTAAGAGAGG ATTCCTCTGG AATGCATCAT GAAAACCAGA CATACCCTCC TTACTCACCA 
CAAGCGCAGC CACAAGCTAT TCATAGAATA GACTCCCCTG GACTTAAGCC AGCCTCTCAA 
CAGAAAGCAG AAGCCTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAC CCCAGCATCA 
TCAGCCTCTG CAGTTAATCA TAATGTCAGT GTAACTAATG TCAGCCTGGA GGAGCCTGCC 
CCAGCCCCTC CCACCTCGCA CGCATCACAG CCTGGTTGTT TAGGAGCACC AAGTGCTGAG 
GCAGCTCACG TAGTGCTCAG AGGTGAAGGA CCACCATTGC CGCCGCATGC AGACCCAGCA 
AAGGTGTACA GGAAGGAGCC GTATTCTGAA GAAATGATGA GACAAAACCA TATTTTAAAA 
CAACCAGCTC TTGGTCACCC AGGGCAGAGG CCAGATAAAG AGCCAAATCT AGCCTATGAA 
CCCCAACTTC CATATATAGA AAAACAAGCC AGCAGAGACC TTGAGCAGCC GTCATACAGG 
TATGAGGTCT CAAGCTACAC AGACCAGTTT TCTCGGAACT ATGACCATCG CCTACGGTTT 
GAAGATCGAA TCCCTACCTA TGAAGACCAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CAACCTCGGC CTTTTGAGAA TCAGCATCCC CGAGACCTGG ACTCCAGACA ACATCCCGAA 
GAGGCTTCAG AACGAGGTTA TTTCCAGCGT TTTGAAGAGC CAGCCCCTCT GTCGTACGAC 
AGTAGAACAC GCTATGAGCA GCTGCCTCGA ACCTCTACTC TACGACATGA AGAGCAGCCA 
GCCCCTGCAT ATGAGGTGCA CAACAGGTAC AGGCCAGAGG CACAGCCCTA TTCTTCAACA 
GGCCCTAAGT CATCTGAGCC CAAGCAGTAC TTTGACCAGT ACCCGCGAAG TTATGAGCAA 
GTTCCACCAC CAGGCTTTAC CTCCAAAACA GGCCATTACG AGCCTCTCCA TGGTGCTGCA 
GTTGTCCCTC CTCTGATACC TTCCTCTCAA CAAAAGCCAG AAGTCCTGCC CTCGGCTACC 
AAACCACAGC CTCCACCCCC AACCCTAACT GAGGAGGAGG AGGATCCAGC AATGAAACCA 
CAGTCTGTGC TCACCAGAGT CAAAATGTTT GAAAACAAAA GATCTGCGTC TTTGGAGAAC 
AAGAAAGATG TGAATGACAC TGCCAGCTTT AAGCCTCCGG AAGTAGCATC TAAACCTCCA 
GGTGCTTCTC TTGCTGGCCC TAAACCTGTC CCTCAGAGTC AGTTTAGTGA GCACGACAAA 
ACGCTCTACA GGCTCCCAGA GCCTCAGAAA CCTCAAGTGA AGCCACCCGA AGATATTGTT 
CGATCAAATC ATTACGACCC TGAAGAGGAT GAAGAATATT ACCGGAAACA GCTCTCCTAC 
TTTGACCGAA GAAGTTTTGA GAGCAAGCCT TCTGCACATC TTCCTGCTGG CCATCACTCA 
GAGCCTGCTA AGCCAGTCCA TTCTCAGAGC CAGCCCAACT TCTCTAGTTA CTCTTCAAAA 
TTTCTTGGCT CTTACACTAG CTATGACTAC TTGAAAAGGA ACATCAAGTG GGGAAAACCC 
GAAACTGATG CTGTGGATAG ATCATTCAGT GAGAAACGTT ATGATCCAGC CCAGGCCACG 
CCTCCTCCTC CTCCGTTGCC CTCACAGTAC AGCCAGCCAG CTCCACCTCT GTCCAGCTCT 
TCTCTCCACA TACATTCCAA GGGCGCCCAG GGTGAAGGCA ACTCAGTATC ATTGGATTTT 
CAGAACTCAT ATATGTCCAA ACCAGACCCA CCCCCATCTC AGAGTAAACC AGCAACTTTC 
AGACCACCAA CTCGAGAAGA CCCCCCTCAG ACTTTCTATC CGCAGAAAAG TTTCCCAGAC 
AAAGCTCCAG TTAACGGAGC TGAGCAGACT CAGAAAACCA TCACTCCGGT GTACAATCGA 
TTCACACCAA AGCCGTACAC AAGTTCTGCC CGGCCATTTG AACGCAAATT TGAAAGTCCG 
AAGTTCAACC ATAATCTTCT GCCAAGTGAA ACTGTACATA AACCTGAATT GTCTTCAAAA 
ACTCCCACTT CCCCAAAAAC TCTTATGAAA GCTCATAGTT CAACACAGCC TCCAGAGTTT 
GACAGTGGAG TTGAAACTTT CTCTGTTCAC ACAGATAAGC CTAAATATCA AATGAATAAT 
ATCAGCACCA TGCCTAAAGC TGTCCCTGTG AGTCCTTCAG CTGTGGAAGA AGATGAAGAT 
GAGGATGGTC ATACTGTAGT GGCTACAGCT CGTGGCATTT TTAACAGCAA TGGTGGTGTG 
TTGAGTTCCA TAGAAACCGG TGTTAGTATA ATTATCCCAC AAGGAGCCAT TCCTGAAGGA 
ATTGAGCAAG AAATCTATTT CAAAGTCTGC AGAGACAATA GCATTCTCCC ACCTTTAGAT 
AAAGAGAAAG GTGAAACTCT GCTGAGCCCC CTAGTGATGT GTGGGCCCCA TGGCCTCAAG 
TTCCTGAAGC CCGTGGAGCT ACGCTTGCCA CACTGTGCGT CCATGACTCC TGACGGTTGG 
TCTTTTGCTC TAAAATCATC CGACTCCTCG TCGGGTGACC CTAAAACCTG GCAAAACAAG 
TGTCTTCCTG GAGATCCGAA TTACCTTGTT GGAGCCAACT GTGTTTCTGT CCTGATTGAC 
CACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006540847.1
CDS	846..6194
Translation	MSARAAAAKSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSHPDPEPVSDNEDDSYDEEVHDPRAGRGALANRRSEKSWARDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSQSRSPDQRSEPSDHSTQSPQQPSNGSLRSREEERMSKPGAISTPVKHVDDHPPKAVEEVTVEKNEKQTPTLPEPKPVYAQVGQPDVDLPVSPSDGALPNSAHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDHRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQAIHRIDSPGLKPASQQKAEASSPVPYLSPETTPASSASAVNHNVSVTNVSLEEPAPAPPTSHASQPGCLGAPSAEAAHVVLRGEGPPLPPHADPAKVYRKEPYSEEMMRQNHILKQPALGHPGQRPDKEPNLAYEPQLPYIEKQASRDLEQPSYRYEVSSYTDQFSRNYDHRLRFEDRIPTYEDQWSYYDDKQPYQPRPFENQHPRDLDSRQHPEEASERGYFQRFEEPAPLSYDSRTRYEQLPRTSTLRHEEQPAPAYEVHNRYRPEAQPYSSTGPKSSEPKQYFDQYPRSYEQVPPPGFTSKTGHYEPLHGAAVVPPLIPSSQQKPEVLPSATKPQPPPPTLTEEEEDPAMKPQSVLTRVKMFENKRSASLENKKDVNDTASFKPPEVASKPPGASLAGPKPVPQSQFSEHDKTLYRLPEPQKPQVKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFESKPSAHLPAGHHSEPAKPVHSQSQPNFSSYSSKFLGSYTSYDYLKRNIKWGKPETDAVDRSFSEKRYDPAQATPPPPPLPSQYSQPAPPLSSSSLHIHSKGAQGEGNSVSLDFQNSYMSKPDPPPSQSKPATFRPPTREDPPQTFYPQKSFPDKAPVNGAEQTQKTITPVYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETVHKPELSSKTPTSPKTLMKAHSSTQPPEFDSGVETFSVHTDKPKYQMNNISTMPKAVPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_006540784.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus tight junction protein 1 (Tjp1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006540784.4

ORF Insert Sequence:

ATGTCCGCCA GGGCCGCGGC CGCTAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAGCACACAG TGACGCTTCA CAGGGCTCCT GGGTTTGGAT TTGGAATTGC AATCTCTGGT 
GGAAGAGATA ATCCTCATTT TCAGAGTGGG GAAACCTCCA TAGTGATTTC TGATGTGTTA 
AAAGGAGGGC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA 
GTTTCAATGG ATAACGTTGA ACATGCTTTT GCTGTTCAGC AGCTAAGGAA GAGTGGGAAA 
AACGCAAAAA TTACTATCCG AAGGAAGAAG AAAGTTCAGA TCCCTGTAAG TCACCCAGAT 
CCTGAGCCGG TGTCTGATAA TGAAGACGAT AGTTATGACG AAGAAGTGCA TGACCCAAGA 
GCTGGCCGCG GTGCTTTAGC GAACAGAAGG AGCGAGAAGA GCTGGGCAAG GGATAGGAGT 
GCAAGCAGGG AGAGGAGCCT GTCCCCTCGC TCGGACAGGC GGTCCGTGGC CTCCAGTCAG 
CCCGCAAAGC CCACCAAGGT CACACTGGTG AAGTCTCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCCA GCCATATATT TGTAAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGTAACATTC AAGAAGGGGA TGTTGTCTTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAAAC ACTGATAGAA AGGTCTAAAG GCAAGTTAAA AATGGTAGTG 
CAAAGAGATG AGCGGGCTAC CTTACTGAAC GTCCCTGACC TTTCGGATAG TATCCATTCT 
GCTAATGCCT CGGAAAGAGA TGACATTTCA GAAATTCAGT CACTAGCATC AGACCATTCA 
GGTCGCTCGC ATGACAGGCC ACCCCGCCGC AGCCAGTCAC GATCTCCTGA CCAACGTTCA 
GAGCCCTCCG ATCATTCCAC GCAGTCTCCA CAGCAGCCCA GCAATGGCAG TCTCCGGAGC 
AGAGAGGAAG AGCGAATGTC TAAACCTGGG GCCATCTCAA CTCCTGTAAA ACATGTAGAC 
GATCATCCAC CCAAAGCAGT GGAAGAAGTT ACAGTTGAGA AAAATGAGAA GCAGACGCCC 
ACTCTTCCAG AACCGAAACC TGTGTATGCT CAAGTTGGAC AACCAGATGT GGATTTACCC 
GTCAGCCCTT CTGATGGTGC TCTGCCTAAT TCAGCTCATG AAGACGGGAT ACTTAGGCCC 
AGCATGAAAC TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGACT AGCTGGTGGA 
AATGATGTCG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAAGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTG AACAATGTAG ATTTCACAAA TATCATAAGG 
GAAGAGGCCG TCCTTTTCCT CCTTGACCTC CCTAAAGGTG AAGAAGTGAC CATACTGGCT 
CAGAAGAAGA AGGACGTTTA TCGCCGCATT GTAGAATCAG ATGTAGGAGA TTCATTCTAT 
ATTAGAACGC ATTTTGAATA TGAAAAAGAA TCTCCTTACG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GGGTCGTGGA TACTTTATAC AATGGAAAGC TGGGCTCTTG GCTTGCCATT 
CGAATTGGCA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAACAGTTAG CCAGTGTACA GTACACACTT CCAAAGACAG CGGGTGGTGA TCGGGCAGAC 
TTCTGGAGGT TTCGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAC 
TTGTCAGCTC AGCCAGTTCA AACTAAGTTC CCAGCTTATG AAAGGGTTGT TCTTCGAGAA 
GCTGGATTCC TAAGACCTGT AACCATCTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
TTGGCAAGAG AGGAGCCAGA TATCTATCAG ATTGCAAAAA GTGAACCACG AGATGCTGGG 
ACTGACCATC GTAGCTCTGG CATCATTCGC CTTCATACAA TAAAGCAAAT CATAGATCAG 
GATAAACATG CTTTATTAGA TGTAACGCCA AATGCGGTTG ATCGTCTTAA TTATGCGCAG 
TGGTATCCAA TTGTTGTGTT CCTTAACCCT GACTCTAAGC AAGGTGTAAA AACAATGAGG 
ATGAGGTTGT GTCCGGAGTC TCGGAAAAGC GCCAGGAAGC TATATGAACG CTCTCATAAG 
CTTCGTAAGA ACAATCACCA TCTCTTCACA ACTACAATTA ACTTAAACTC AATGAATGAT 
GGTTGGTACG GTGCCCTGAA AGAAGCGATT CAGCAGCAAC AGAACCAGCT GGTGTGGGTC 
TCTGAGGGGA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA CGATCGTCTG 
TCCTACCTGT CAGCCCCAGG TAGTGAGTAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA TACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTGGG GACTCCCCCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTAAGAGAGG ATTCCTCTGG AATGCATCAT GAAAACCAGA CATACCCTCC TTACTCACCA 
CAAGCGCAGC CACAAGCTAT TCATAGAATA GACTCCCCTG GACTTAAGCC AGCCTCTCAA 
CAGAAAGCAG AAGCCTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAC CCCAGCATCA 
TCAGCCTCTG CAGTTAATCA TAATGTCAGT GTAACTAATG TCAGCCTGGA GGAGCCTGCC 
CCAGCCCCTC CCACCTCGCA CGCATCACAG CCTGGTTGTT TAGGAGCACC AAGTGCTGAG 
GCAGCTCACG TAGTGCTCAG AGGTGAAGGA CCACCATTGC CGCCGCATGC AGACCCAGCA 
AAGGTGTACA GGAAGGAGCC GTATTCTGAA GAAATGATGA GACAAAACCA TATTTTAAAA 
CAACCAGCTC TTGGTCACCC AGGGCAGAGG CCAGATAAAG AGCCAAATCT AGCCTATGAA 
CCCCAACTTC CATATATAGA AAAACAAGCC AGCAGAGACC TTGAGCAGCC GTCATACAGG 
TATGAGGTCT CAAGCTACAC AGACCAGTTT TCTCGGAACT ATGACCATCG CCTACGGTTT 
GAAGATCGAA TCCCTACCTA TGAAGACCAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CAACCTCGGC CTTTTGAGAA TCAGCATCCC CGAGACCTGG ACTCCAGACA ACATCCCGAA 
GAGGCTTCAG AACGAGGTTA TTTCCAGCGT TTTGAAGAGC CAGCCCCTCT GTCGTACGAC 
AGTAGAACAC GCTATGAGCA GCTGCCTCGA ACCTCTACTC TACGACATGA AGAGCAGCCA 
GCCCCTGCAT ATGAGGTGCA CAACAGGTAC AGGCCAGAGG CACAGCCCTA TTCTTCAACA 
GGCCCTAAGT CATCTGAGCC CAAGCAGTAC TTTGACCAGT ACCCGCGAAG TTATGAGCAA 
GTTCCACCAC CAGGCTTTAC CTCCAAAACA GGCCATTACG AGCCTCTCCA TGGTGCTGCA 
GTTGTCCCTC CTCTGATACC TTCCTCTCAA CAAAAGCCAG AAGTCCTGCC CTCGGCTACC 
AAACCACAGC CTCCACCCCC AACCCTAACT GAGGAGGAGG AGGATCCAGC AATGAAACCA 
CAGTCTGTGC TCACCAGAGT CAAAATGTTT GAAAACAAAA GATCTGCGTC TTTGGAGAAC 
AAGAAAGATG TGAATGACAC TGCCAGCTTT AAGCCTCCGG AAGTAGCATC TAAACCTCCA 
GGTGCTTCTC TTGCTGGCCC TAAACCTGTC CCTCAGAGTC AGTTTAGTGA GCACGACAAA 
ACGCTCTACA GGCTCCCAGA GCCTCAGAAA CCTCAAGTGA AGCCACCCGA AGATATTGTT 
CGATCAAATC ATTACGACCC TGAAGAGGAT GAAGAATATT ACCGGAAACA GCTCTCCTAC 
TTTGACCGAA GAAGTTTTGA GAGCAAGCCT TCTGCACATC TTCCTGCTGG CCATCACTCA 
GAGCCTGCTA AGCCAGTCCA TTCTCAGAGC CAGCCCAACT TCTCTAGTTA CTCTTCAAAA 
TTTCTTGGCT CTTACACTAG CTATGACTAC TTGAAAAGGA ACATCAAGTG GGGAAAACCC 
GAAACTGATG CTGTGGATAG ATCATTCAGT GAGAAACGTT ATGATCCAGC CCAGGCCACG 
CCTCCTCCTC CTCCGTTGCC CTCACAGTAC AGCCAGCCAG CTCCACCTCT GTCCAGCTCT 
TCTCTCCACA TACATTCCAA GGGCGCCCAG GGTGAAGGCA ACTCAGTATC ATTGGATTTT 
CAGAACTCAT ATATGTCCAA ACCAGACCCA CCCCCATCTC AGAGTAAACC AGCAACTTTC 
AGACCACCAA CTCGAGAAGA CCCCCCTCAG ACTTTCTATC CGCAGAAAAG TTTCCCAGAC 
AAAGCTCCAG TTAACGGAGC TGAGCAGACT CAGAAAACCA TCACTCCGGT GTACAATCGA 
TTCACACCAA AGCCGTACAC AAGTTCTGCC CGGCCATTTG AACGCAAATT TGAAAGTCCG 
AAGTTCAACC ATAATCTTCT GCCAAGTGAA ACTGTACATA AACCTGAATT GTCTTCAAAA 
ACTCCCACTT CCCCAAAAAC TCTTATGAAA GCTCATAGTT CAACACAGCC TCCAGAGTTT 
GACAGTGGAG TTGAAACTTT CTCTGTTCAC ACAGATAAGC CTAAATATCA AATGAATAAT 
ATCAGCACCA TGCCTAAAGC TGTCCCTGTG AGTCCTTCAG CTGTGGAAGA AGATGAAGAT 
GAGGATGGTC ATACTGTAGT GGCTACAGCT CGTGGCATTT TTAACAGCAA TGGTGGTGTG 
TTGAGTTCCA TAGAAACCGG TGTTAGTATA ATTATCCCAC AAGGAGCCAT TCCTGAAGGA 
ATTGAGCAAG AAATCTATTT CAAAGTCTGC AGAGACAATA GCATTCTCCC ACCTTTAGAT 
AAAGAGAAAG GTGAAACTCT GCTGAGCCCC CTAGTGATGT GTGGGCCCCA TGGCCTCAAG 
TTCCTGAAGC CCGTGGAGCT ACGCTTGCCA CACTGTGCGT CCATGACTCC TGACGGTTGG 
TCTTTTGCTC TAAAATCATC CGACTCCTCG TCGGGTGACC CTAAAACCTG GCAAAACAAG 
TGTCTTCCTG GAGATCCGAA TTACCTTGTT GGAGCCAACT GTGTTTCTGT CCTGATTGAC 
CACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tjp1 ( XM_006540785.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006540848.1 Mus musculus tight junction protein 1 (Tjp1), transcript variant X9, mRNA.

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CloneID	OMu38775
Clone ID Related Accession (Same CDS sequence)	XM_006540785.3 , XM_006540785.4
Accession Version	XM_006540785.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5337bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tight junction protein ZO-1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006540785.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT 
CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA 
CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC 
AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA 
CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA 
AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT 
GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG 
AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG 
TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC 
ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT 
GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT 
GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA 
CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC 
TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT 
GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA 
CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG 
CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT 
AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG 
GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT 
GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT 
CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC 
CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT 
CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT 
GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT 
ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG 
TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA 
ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA 
ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT 
ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC 
ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT 
GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC 
CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT 
CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT 
CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA 
GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT 
AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG 
ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA 
ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT 
CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGGTGTACAG GAAGGAGCCG 
TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA 
GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA 
AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA 
GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT 
GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT 
CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT 
TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG 
CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC 
AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC 
AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC 
TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT 
TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA 
ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC 
AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT 
GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT 
AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG 
CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT 
GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG 
AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT 
TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAGG GAAAACCCGA AACTGATGCT 
GTGGATAGAT CATTCAGTGA GAAACGTTAT GATCCAGCCC AGGCCACGCC TCCTCCTCCT 
CCGTTGCCCT CACAGTACAG CCAGCCAGCT CCACCTCTGT CCAGCTCTTC TCTCCACATA 
CATTCCAAGG GCGCCCAGGG TGAAGGCAAC TCAGTATCAT TGGATTTTCA GAACTCATAT 
ATGTCCAAAC CAGACCCACC CCCATCTCAG AGTAAACCAG CAACTTTCAG ACCACCAACT 
CGAGAAGACC CCCCTCAGAC TTTCTATCCG CAGAAAAGTT TCCCAGACAA AGCTCCAGTT 
AACGGAGCTG AGCAGACTCA GAAAACCATC ACTCCGGTGT ACAATCGATT CACACCAAAG 
CCGTACACAA GTTCTGCCCG GCCATTTGAA CGCAAATTTG AAAGTCCGAA GTTCAACCAT 
AATCTTCTGC CAAGTGAAAC TGTACATAAA CCTGAATTGT CTTCAAAAAC TCCCACTTCC 
CCAAAAACTC TTATGAAAGC TCATAGTTCA ACACAGCCTC CAGAGTTTGA CAGTGGAGTT 
GAAACTTTCT CTGTTCACAC AGATAAGCCT AAATATCAAA TGAATAATAT CAGCACCATG 
CCTAAAGCTG TCCCTGTGAG TCCTTCAGCT GTGGAAGAAG ATGAAGATGA GGATGGTCAT 
ACTGTAGTGG CTACAGCTCG TGGCATTTTT AACAGCAATG GTGGTGTGTT GAGTTCCATA 
GAAACCGGTG TTAGTATAAT TATCCCACAA GGAGCCATTC CTGAAGGAAT TGAGCAAGAA 
ATCTATTTCA AAGTCTGCAG AGACAATAGC ATTCTCCCAC CTTTAGATAA AGAGAAAGGT 
GAAACTCTGC TGAGCCCCCT AGTGATGTGT GGGCCCCATG GCCTCAAGTT CCTGAAGCCC 
GTGGAGCTAC GCTTGCCACA CTGTGCGTCC ATGACTCCTG ACGGTTGGTC TTTTGCTCTA 
AAATCATCCG ACTCCTCGTC GGGTGACCCT AAAACCTGGC AAAACAAGTG TCTTCCTGGA 
GATCCGAATT ACCTTGTTGG AGCCAACTGT GTTTCTGTCC TGATTGACCA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006540848.1
CDS	115..5451
Translation	MRRLEGLCLRRRRRRRGPARERSGDKMKYQKYLTVLQMAIGVTPSNRGSLLPLKRKLWVTPSSENPHGATSTVSQGKPSLRRIKGRLHRSKSLDSIDFCELTSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSHPDPEPVSDNEDDSYDEEVHDPRAGRGALANRRSEKSWARDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSQSRSPDQRSEPSDHSTQSPQQPSNGSLRSREEERMSKPGAISTPVKHVDDHPPKAVEEVTVEKNEKQTPTLPEPKPVYAQVGQPDVDLPVSPSDGALPNSAHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDHRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQAIHRIDSPGLKPASQQVYRKEPYSEEMMRQNHILKQPALGHPGQRPDKEPNLAYEPQLPYIEKQASRDLEQPSYRYEVSSYTDQFSRNYDHRLRFEDRIPTYEDQWSYYDDKQPYQPRPFENQHPRDLDSRQHPEEASERGYFQRFEEPAPLSYDSRTRYEQLPRTSTLRHEEQPAPAYEVHNRYRPEAQPYSSTGPKSSEPKQYFDQYPRSYEQVPPPGFTSKTGHYEPLHGAAVVPPLIPSSQQKPEVLPSATKPQPPPPTLTEEEEDPAMKPQSVLTRVKMFENKRSASLENKKDVNDTASFKPPEVASKPPGASLAGPKPVPQSQFSEHDKTLYRLPEPQKPQVKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFESKPSAHLPAGHHSEPAKPVHSQSQPNFSSYSSKGKPETDAVDRSFSEKRYDPAQATPPPPPLPSQYSQPAPPLSSSSLHIHSKGAQGEGNSVSLDFQNSYMSKPDPPPSQSKPATFRPPTREDPPQTFYPQKSFPDKAPVNGAEQTQKTITPVYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETVHKPELSSKTPTSPKTLMKAHSSTQPPEFDSGVETFSVHTDKPKYQMNNISTMPKAVPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_006540785.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus tight junction protein 1 (Tjp1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006540785.4

ORF Insert Sequence:

ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT 
CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA 
CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC 
AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA 
CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA 
AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT 
GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG 
AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG 
TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC 
ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT 
GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT 
GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA 
CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC 
TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT 
GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA 
CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG 
CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT 
AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG 
GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT 
GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT 
CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC 
CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT 
CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT 
GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT 
ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG 
TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA 
ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA 
ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT 
ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC 
ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT 
GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC 
CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT 
CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT 
CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA 
GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT 
AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG 
ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA 
ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT 
CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGGTGTACAG GAAGGAGCCG 
TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA 
GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA 
AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA 
GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT 
GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT 
CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT 
TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG 
CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC 
AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC 
AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC 
TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT 
TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA 
ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC 
AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT 
GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT 
AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG 
CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT 
GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG 
AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT 
TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAGG GAAAACCCGA AACTGATGCT 
GTGGATAGAT CATTCAGTGA GAAACGTTAT GATCCAGCCC AGGCCACGCC TCCTCCTCCT 
CCGTTGCCCT CACAGTACAG CCAGCCAGCT CCACCTCTGT CCAGCTCTTC TCTCCACATA 
CATTCCAAGG GCGCCCAGGG TGAAGGCAAC TCAGTATCAT TGGATTTTCA GAACTCATAT 
ATGTCCAAAC CAGACCCACC CCCATCTCAG AGTAAACCAG CAACTTTCAG ACCACCAACT 
CGAGAAGACC CCCCTCAGAC TTTCTATCCG CAGAAAAGTT TCCCAGACAA AGCTCCAGTT 
AACGGAGCTG AGCAGACTCA GAAAACCATC ACTCCGGTGT ACAATCGATT CACACCAAAG 
CCGTACACAA GTTCTGCCCG GCCATTTGAA CGCAAATTTG AAAGTCCGAA GTTCAACCAT 
AATCTTCTGC CAAGTGAAAC TGTACATAAA CCTGAATTGT CTTCAAAAAC TCCCACTTCC 
CCAAAAACTC TTATGAAAGC TCATAGTTCA ACACAGCCTC CAGAGTTTGA CAGTGGAGTT 
GAAACTTTCT CTGTTCACAC AGATAAGCCT AAATATCAAA TGAATAATAT CAGCACCATG 
CCTAAAGCTG TCCCTGTGAG TCCTTCAGCT GTGGAAGAAG ATGAAGATGA GGATGGTCAT 
ACTGTAGTGG CTACAGCTCG TGGCATTTTT AACAGCAATG GTGGTGTGTT GAGTTCCATA 
GAAACCGGTG TTAGTATAAT TATCCCACAA GGAGCCATTC CTGAAGGAAT TGAGCAAGAA 
ATCTATTTCA AAGTCTGCAG AGACAATAGC ATTCTCCCAC CTTTAGATAA AGAGAAAGGT 
GAAACTCTGC TGAGCCCCCT AGTGATGTGT GGGCCCCATG GCCTCAAGTT CCTGAAGCCC 
GTGGAGCTAC GCTTGCCACA CTGTGCGTCC ATGACTCCTG ACGGTTGGTC TTTTGCTCTA 
AAATCATCCG ACTCCTCGTC GGGTGACCCT AAAACCTGGC AAAACAAGTG TCTTCCTGGA 
GATCCGAATT ACCTTGTTGG AGCCAACTGT GTTTCTGTCC TGATTGACCA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tjp1 ( XM_006540786.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006540849.1 Mus musculus tight junction protein 1 (Tjp1), transcript variant X11, mRNA.

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CloneID	OMu38776
Clone ID Related Accession (Same CDS sequence)	XM_006540786.4 , XM_006540786.3
Accession Version	XM_006540786.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5277bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tight junction protein ZO-1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006540786.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT 
CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA 
CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC 
AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA 
CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA 
AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT 
GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG 
AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG 
TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC 
ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT 
GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT 
GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA 
CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC 
TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT 
GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA 
CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG 
CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT 
AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG 
GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT 
GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT 
CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC 
CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT 
CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT 
GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT 
ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG 
TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA 
ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA 
ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT 
ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC 
ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT 
GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC 
CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT 
CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT 
CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA 
GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT 
AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG 
ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA 
ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT 
CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGGTGTACAG GAAGGAGCCG 
TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA 
GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA 
AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA 
GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT 
GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT 
CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT 
TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG 
CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC 
AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC 
AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC 
TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT 
TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA 
ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC 
AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT 
GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT 
AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG 
CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT 
GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG 
AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT 
TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAGG GAAAACCCGA AACTGATGCT 
GTGGATAGAT CATTCAGTGA GAAACGTTAT GATCCAGCCC AGGCCACGCC TCCTCCTCCT 
CCGTTGCCCT CACAGTACAG CCAGCCAGCT CCACCTCTGT CCAGCTCTTC TCTCCACATA 
CATTCCAAGG GCGCCCAGGG TGAAGGCAAC TCAGTATCAT TGGATTTTCA GAACTCATAT 
ATGTCCAAAC CAGACCCACC CCCATCTCAG AGTAAACCAG CAACTTTCAG ACCACCAACT 
CGAGAAGACC CCCCTCAGAC TTTCTATCCG CAGAAAAGTT TCCCAGACAA AGCTCCAGTT 
AACGGAGCTG AGCAGACTCA GAAAACCATC ACTCCGGTGT ACAATCGATT CACACCAAAG 
CCGTACACAA GTTCTGCCCG GCCATTTGAA CGCAAATTTG AAAGTCCGAA GTTCAACCAT 
AATCTTCTGC CAAGTGAAAC TGTACATAAA CCTGAATTGT CTTCAAAAAC TCCCACTTCC 
CCAAAAACTC TTATGAAAGC TCATAGTTCA ACACAGCCTC CAGAGTTTGA CAGTGGAGTT 
GAAACTTTCT CTGTTCACAC AGATAAGCCT AAATATCAAA TGAATAATAT CAGCACCATG 
CCTAAAGCTG TCCCTGTGAG TCCTTCAGCT GTGGAAGAAG ATGAAGATGA GGATGGTCAT 
ACTGTAGTGG CTACAGCTCG TGGCATTTTT AACAGCAATG GTGGTGTGTT GAGTTCCATA 
GAAACCGGTG TTAGTATAAT TATCCCACAA GGAGCCATTC CTGAAGGAAT TGAGCAAGAA 
ATCTATTTCA AAGTCTGCAG AGACAATAGC ATTCTCCCAC CTTTAGATAA AGAGAAAGGT 
GAAACTCTGC TGAGCCCCCT AGTGATGTGT GGGCCCCATG GCCTCAAGTT CCTGAAGCCC 
GTGGAGCTAC GCTTGCCACA CTGTGACCCT AAAACCTGGC AAAACAAGTG TCTTCCTGGA 
GATCCGAATT ACCTTGTTGG AGCCAACTGT GTTTCTGTCC TGATTGACCA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006540849.1
CDS	115..5391
Translation	MRRLEGLCLRRRRRRRGPARERSGDKMKYQKYLTVLQMAIGVTPSNRGSLLPLKRKLWVTPSSENPHGATSTVSQGKPSLRRIKGRLHRSKSLDSIDFCELTSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSHPDPEPVSDNEDDSYDEEVHDPRAGRGALANRRSEKSWARDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSQSRSPDQRSEPSDHSTQSPQQPSNGSLRSREEERMSKPGAISTPVKHVDDHPPKAVEEVTVEKNEKQTPTLPEPKPVYAQVGQPDVDLPVSPSDGALPNSAHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDHRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQAIHRIDSPGLKPASQQVYRKEPYSEEMMRQNHILKQPALGHPGQRPDKEPNLAYEPQLPYIEKQASRDLEQPSYRYEVSSYTDQFSRNYDHRLRFEDRIPTYEDQWSYYDDKQPYQPRPFENQHPRDLDSRQHPEEASERGYFQRFEEPAPLSYDSRTRYEQLPRTSTLRHEEQPAPAYEVHNRYRPEAQPYSSTGPKSSEPKQYFDQYPRSYEQVPPPGFTSKTGHYEPLHGAAVVPPLIPSSQQKPEVLPSATKPQPPPPTLTEEEEDPAMKPQSVLTRVKMFENKRSASLENKKDVNDTASFKPPEVASKPPGASLAGPKPVPQSQFSEHDKTLYRLPEPQKPQVKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFESKPSAHLPAGHHSEPAKPVHSQSQPNFSSYSSKGKPETDAVDRSFSEKRYDPAQATPPPPPLPSQYSQPAPPLSSSSLHIHSKGAQGEGNSVSLDFQNSYMSKPDPPPSQSKPATFRPPTREDPPQTFYPQKSFPDKAPVNGAEQTQKTITPVYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETVHKPELSSKTPTSPKTLMKAHSSTQPPEFDSGVETFSVHTDKPKYQMNNISTMPKAVPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_006540786.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus tight junction protein 1 (Tjp1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006540786.4

ORF Insert Sequence:

ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT 
CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA 
CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC 
AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA 
CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA 
AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT 
GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG 
AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG 
TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC 
ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT 
GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT 
GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA 
CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC 
TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT 
GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA 
CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG 
CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT 
AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG 
GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT 
GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT 
CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC 
CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT 
CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT 
GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT 
ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG 
TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA 
ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA 
ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT 
ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC 
ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT 
GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC 
CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT 
CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT 
CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA 
GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT 
AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG 
ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA 
ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT 
CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGGTGTACAG GAAGGAGCCG 
TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA 
GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA 
AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA 
GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT 
GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT 
CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT 
TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG 
CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC 
AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC 
AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC 
TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT 
TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA 
ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC 
AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT 
GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT 
AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG 
CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT 
GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG 
AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT 
TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAGG GAAAACCCGA AACTGATGCT 
GTGGATAGAT CATTCAGTGA GAAACGTTAT GATCCAGCCC AGGCCACGCC TCCTCCTCCT 
CCGTTGCCCT CACAGTACAG CCAGCCAGCT CCACCTCTGT CCAGCTCTTC TCTCCACATA 
CATTCCAAGG GCGCCCAGGG TGAAGGCAAC TCAGTATCAT TGGATTTTCA GAACTCATAT 
ATGTCCAAAC CAGACCCACC CCCATCTCAG AGTAAACCAG CAACTTTCAG ACCACCAACT 
CGAGAAGACC CCCCTCAGAC TTTCTATCCG CAGAAAAGTT TCCCAGACAA AGCTCCAGTT 
AACGGAGCTG AGCAGACTCA GAAAACCATC ACTCCGGTGT ACAATCGATT CACACCAAAG 
CCGTACACAA GTTCTGCCCG GCCATTTGAA CGCAAATTTG AAAGTCCGAA GTTCAACCAT 
AATCTTCTGC CAAGTGAAAC TGTACATAAA CCTGAATTGT CTTCAAAAAC TCCCACTTCC 
CCAAAAACTC TTATGAAAGC TCATAGTTCA ACACAGCCTC CAGAGTTTGA CAGTGGAGTT 
GAAACTTTCT CTGTTCACAC AGATAAGCCT AAATATCAAA TGAATAATAT CAGCACCATG 
CCTAAAGCTG TCCCTGTGAG TCCTTCAGCT GTGGAAGAAG ATGAAGATGA GGATGGTCAT 
ACTGTAGTGG CTACAGCTCG TGGCATTTTT AACAGCAATG GTGGTGTGTT GAGTTCCATA 
GAAACCGGTG TTAGTATAAT TATCCCACAA GGAGCCATTC CTGAAGGAAT TGAGCAAGAA 
ATCTATTTCA AAGTCTGCAG AGACAATAGC ATTCTCCCAC CTTTAGATAA AGAGAAAGGT 
GAAACTCTGC TGAGCCCCCT AGTGATGTGT GGGCCCCATG GCCTCAAGTT CCTGAAGCCC 
GTGGAGCTAC GCTTGCCACA CTGTGACCCT AAAACCTGGC AAAACAAGTG TCTTCCTGGA 
GATCCGAATT ACCTTGTTGG AGCCAACTGT GTTTCTGTCC TGATTGACCA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tjp1 ( XM_011250843.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011249145.1 Mus musculus tight junction protein 1 (Tjp1), transcript variant X5, mRNA.

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CloneID	OMu68895
Clone ID Related Accession (Same CDS sequence)	XM_011250843.2 , XM_011250843.3
Accession Version	XM_011250843.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5538bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tight junction protein ZO-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011250843.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT 
CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA 
CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC 
AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA 
CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA 
AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT 
GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG 
AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG 
TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC 
ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT 
GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT 
GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA 
CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC 
TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT 
GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA 
CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG 
CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT 
AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG 
GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT 
GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT 
CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC 
CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT 
CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT 
GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT 
ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG 
TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA 
ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA 
ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT 
ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC 
ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT 
GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC 
CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT 
CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT 
CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA 
GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT 
AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG 
ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA 
ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT 
CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCCTCATCT 
CCAGTCCCTT ACCTTTCGCC TGAAACAACC CCAGCATCAT CAGCCTCTGC AGTTAATCAT 
AATGTCAGTG TAACTAATGT CAGCCTGGAG GAGCCTGCCC CAGCCCCTCC CACCTCGCAC 
GCATCACAGC CTGGTTGTTT AGGAGCACCA AGTGCTGAGG CAGCTCACGT AGTGCTCAGA 
GGTGAAGGAC CACCATTGCC GCCGCATGCA GACCCAGCAA AGGTGTACAG GAAGGAGCCG 
TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA 
GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA 
AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA 
GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT 
GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT 
CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT 
TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG 
CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC 
AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC 
AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC 
TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT 
TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA 
ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC 
AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT 
GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT 
AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG 
CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT 
GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG 
AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT 
TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAGG GAAAACCCGA AACTGATGCT 
GTGGATAGAT CATTCAGTGA GAAACGTTAT GATCCAGCCC AGGCCACGCC TCCTCCTCCT 
CCGTTGCCCT CACAGTACAG CCAGCCAGCT CCACCTCTGT CCAGCTCTTC TCTCCACATA 
CATTCCAAGG GCGCCCAGGG TGAAGGCAAC TCAGTATCAT TGGATTTTCA GAACTCATAT 
ATGTCCAAAC CAGACCCACC CCCATCTCAG AGTAAACCAG CAACTTTCAG ACCACCAACT 
CGAGAAGACC CCCCTCAGAC TTTCTATCCG CAGAAAAGTT TCCCAGACAA AGCTCCAGTT 
AACGGAGCTG AGCAGACTCA GAAAACCATC ACTCCGGTGT ACAATCGATT CACACCAAAG 
CCGTACACAA GTTCTGCCCG GCCATTTGAA CGCAAATTTG AAAGTCCGAA GTTCAACCAT 
AATCTTCTGC CAAGTGAAAC TGTACATAAA CCTGAATTGT CTTCAAAAAC TCCCACTTCC 
CCAAAAACTC TTATGAAAGC TCATAGTTCA ACACAGCCTC CAGAGTTTGA CAGTGGAGTT 
GAAACTTTCT CTGTTCACAC AGATAAGCCT AAATATCAAA TGAATAATAT CAGCACCATG 
CCTAAAGCTG TCCCTGTGAG TCCTTCAGCT GTGGAAGAAG ATGAAGATGA GGATGGTCAT 
ACTGTAGTGG CTACAGCTCG TGGCATTTTT AACAGCAATG GTGGTGTGTT GAGTTCCATA 
GAAACCGGTG TTAGTATAAT TATCCCACAA GGAGCCATTC CTGAAGGAAT TGAGCAAGAA 
ATCTATTTCA AAGTCTGCAG AGACAATAGC ATTCTCCCAC CTTTAGATAA AGAGAAAGGT 
GAAACTCTGC TGAGCCCCCT AGTGATGTGT GGGCCCCATG GCCTCAAGTT CCTGAAGCCC 
GTGGAGCTAC GCTTGCCACA CTGTGCGTCC ATGACTCCTG ACGGTGACCC TAAAACCTGG 
CAAAACAAGT GTCTTCCTGG AGATCCGAAT TACCTTGTTG GAGCCAACTG TGTTTCTGTC 
CTGATTGACC ACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011249145.1
CDS	115..5652
Translation	MRRLEGLCLRRRRRRRGPARERSGDKMKYQKYLTVLQMAIGVTPSNRGSLLPLKRKLWVTPSSENPHGATSTVSQGKPSLRRIKGRLHRSKSLDSIDFCELTSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSHPDPEPVSDNEDDSYDEEVHDPRAGRGALANRRSEKSWARDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSQSRSPDQRSEPSDHSTQSPQQPSNGSLRSREEERMSKPGAISTPVKHVDDHPPKAVEEVTVEKNEKQTPTLPEPKPVYAQVGQPDVDLPVSPSDGALPNSAHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDHRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQAIHRIDSPGLKPASQQKAEASSPVPYLSPETTPASSASAVNHNVSVTNVSLEEPAPAPPTSHASQPGCLGAPSAEAAHVVLRGEGPPLPPHADPAKVYRKEPYSEEMMRQNHILKQPALGHPGQRPDKEPNLAYEPQLPYIEKQASRDLEQPSYRYEVSSYTDQFSRNYDHRLRFEDRIPTYEDQWSYYDDKQPYQPRPFENQHPRDLDSRQHPEEASERGYFQRFEEPAPLSYDSRTRYEQLPRTSTLRHEEQPAPAYEVHNRYRPEAQPYSSTGPKSSEPKQYFDQYPRSYEQVPPPGFTSKTGHYEPLHGAAVVPPLIPSSQQKPEVLPSATKPQPPPPTLTEEEEDPAMKPQSVLTRVKMFENKRSASLENKKDVNDTASFKPPEVASKPPGASLAGPKPVPQSQFSEHDKTLYRLPEPQKPQVKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFESKPSAHLPAGHHSEPAKPVHSQSQPNFSSYSSKGKPETDAVDRSFSEKRYDPAQATPPPPPLPSQYSQPAPPLSSSSLHIHSKGAQGEGNSVSLDFQNSYMSKPDPPPSQSKPATFRPPTREDPPQTFYPQKSFPDKAPVNGAEQTQKTITPVYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETVHKPELSSKTPTSPKTLMKAHSSTQPPEFDSGVETFSVHTDKPKYQMNNISTMPKAVPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_011250843.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus tight junction protein 1 (Tjp1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011250843.3

ORF Insert Sequence:

ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT 
CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA 
CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC 
AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA 
CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA 
AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT 
GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG 
AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG 
TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC 
ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT 
GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT 
GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA 
CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC 
TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT 
GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA 
CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG 
CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT 
AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG 
GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT 
GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT 
CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC 
CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT 
CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT 
GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT 
ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG 
TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA 
ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA 
ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT 
ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC 
ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT 
GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC 
CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT 
CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT 
CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA 
GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT 
AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG 
ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA 
ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT 
CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCCTCATCT 
CCAGTCCCTT ACCTTTCGCC TGAAACAACC CCAGCATCAT CAGCCTCTGC AGTTAATCAT 
AATGTCAGTG TAACTAATGT CAGCCTGGAG GAGCCTGCCC CAGCCCCTCC CACCTCGCAC 
GCATCACAGC CTGGTTGTTT AGGAGCACCA AGTGCTGAGG CAGCTCACGT AGTGCTCAGA 
GGTGAAGGAC CACCATTGCC GCCGCATGCA GACCCAGCAA AGGTGTACAG GAAGGAGCCG 
TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA 
GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA 
AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA 
GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT 
GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT 
CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT 
TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG 
CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC 
AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC 
AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC 
TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT 
TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA 
ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC 
AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT 
GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT 
AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG 
CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT 
GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG 
AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT 
TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAGG GAAAACCCGA AACTGATGCT 
GTGGATAGAT CATTCAGTGA GAAACGTTAT GATCCAGCCC AGGCCACGCC TCCTCCTCCT 
CCGTTGCCCT CACAGTACAG CCAGCCAGCT CCACCTCTGT CCAGCTCTTC TCTCCACATA 
CATTCCAAGG GCGCCCAGGG TGAAGGCAAC TCAGTATCAT TGGATTTTCA GAACTCATAT 
ATGTCCAAAC CAGACCCACC CCCATCTCAG AGTAAACCAG CAACTTTCAG ACCACCAACT 
CGAGAAGACC CCCCTCAGAC TTTCTATCCG CAGAAAAGTT TCCCAGACAA AGCTCCAGTT 
AACGGAGCTG AGCAGACTCA GAAAACCATC ACTCCGGTGT ACAATCGATT CACACCAAAG 
CCGTACACAA GTTCTGCCCG GCCATTTGAA CGCAAATTTG AAAGTCCGAA GTTCAACCAT 
AATCTTCTGC CAAGTGAAAC TGTACATAAA CCTGAATTGT CTTCAAAAAC TCCCACTTCC 
CCAAAAACTC TTATGAAAGC TCATAGTTCA ACACAGCCTC CAGAGTTTGA CAGTGGAGTT 
GAAACTTTCT CTGTTCACAC AGATAAGCCT AAATATCAAA TGAATAATAT CAGCACCATG 
CCTAAAGCTG TCCCTGTGAG TCCTTCAGCT GTGGAAGAAG ATGAAGATGA GGATGGTCAT 
ACTGTAGTGG CTACAGCTCG TGGCATTTTT AACAGCAATG GTGGTGTGTT GAGTTCCATA 
GAAACCGGTG TTAGTATAAT TATCCCACAA GGAGCCATTC CTGAAGGAAT TGAGCAAGAA 
ATCTATTTCA AAGTCTGCAG AGACAATAGC ATTCTCCCAC CTTTAGATAA AGAGAAAGGT 
GAAACTCTGC TGAGCCCCCT AGTGATGTGT GGGCCCCATG GCCTCAAGTT CCTGAAGCCC 
GTGGAGCTAC GCTTGCCACA CTGTGCGTCC ATGACTCCTG ACGGTGACCC TAAAACCTGG 
CAAAACAAGT GTCTTCCTGG AGATCCGAAT TACCTTGTTG GAGCCAACTG TGTTTCTGTC 
CTGATTGACC ACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tjp1 ( XM_030242356.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030098216.1 Mus musculus tight junction protein 1 (Tjp1), transcript variant X10, mRNA.

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CloneID	OMu89424
Clone ID Related Accession (Same CDS sequence)	XM_030242356.1
Accession Version	XM_030242356.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5313bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tight junction protein ZO-1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAAA CAGCTATATG GGAACAGCAC ACAGTGACGC TTCACAGGGC TCCTGGGTTT 
GGATTTGGAA TTGCAATCTC TGGTGGAAGA GATAATCCTC ATTTTCAGAG TGGGGAAACC 
TCCATAGTGA TTTCTGATGT GTTAAAAGGA GGGCCAGCTG AAGGACAGCT ACAGGAAAAT 
GACCGAGTTG CAATGGTTAA CGGAGTTTCA ATGGATAACG TTGAACATGC TTTTGCTGTT 
CAGCAGCTAA GGAAGAGTGG GAAAAACGCA AAAATTACTA TCCGAAGGAA GAAGAAAGTT 
CAGATCCCTG TAAGTCACCC AGATCCTGAG CCGGTGTCTG ATAATGAAGA CGATAGTTAT 
GACGAAGAAG TGCATGACCC AAGAGCTGGC CGCGGTGCTT TAGCGAACAG AAGGAGCGAG 
AAGAGCTGGG CAAGGGATAG GAGTGCAAGC AGGGAGAGGA GCCTGTCCCC TCGCTCGGAC 
AGGCGGTCCG TGGCCTCCAG TCAGCCCGCA AAGCCCACCA AGGTCACACT GGTGAAGTCT 
CGGAAAAATG AAGAATATGG TCTTCGATTG GCCAGCCATA TATTTGTAAA GGAAATTTCA 
CAAGATAGTT TGGCAGCAAG AGATGGTAAC ATTCAAGAAG GGGATGTTGT CTTGAAGATA 
AATGGTACTG TGACAGAAAA TATGTCATTG ACAGATGCAA AAACACTGAT AGAAAGGTCT 
AAAGGCAAGT TAAAAATGGT AGTGCAAAGA GATGAGCGGG CTACCTTACT GAACGTCCCT 
GACCTTTCGG ATAGTATCCA TTCTGCTAAT GCCTCGGAAA GAGATGACAT TTCAGAAATT 
CAGTCACTAG CATCAGACCA TTCAGGTCGC TCGCATGACA GGCCACCCCG CCGCAGCCAG 
TCACGATCTC CTGACCAACG TTCAGAGCCC TCCGATCATT CCACGCAGTC TCCACAGCAG 
CCCAGCAATG GCAGTCTCCG GAGCAGAGAG GAAGAGCGAA TGTCTAAACC TGGGGCCATC 
TCAACTCCTG TAAAACATGT AGACGATCAT CCACCCAAAG CAGTGGAAGA AGTTACAGTT 
GAGAAAAATG AGAAGCAGAC GCCCACTCTT CCAGAACCGA AACCTGTGTA TGCTCAAGTT 
GGACAACCAG ATGTGGATTT ACCCGTCAGC CCTTCTGATG GTGCTCTGCC TAATTCAGCT 
CATGAAGACG GGATACTTAG GCCCAGCATG AAACTGGTAA AATTCAGAAA AGGAGATAGT 
GTGGGTTTGC GACTAGCTGG TGGAAATGAT GTCGGAATAT TTGTAGCTGG CGTTCTAGAA 
GATAGCCCTG CAGCCAAAGA AGGCTTAGAG GAAGGTGATC AAATTCTCAG GGTGAACAAT 
GTAGATTTCA CAAATATCAT AAGGGAAGAG GCCGTCCTTT TCCTCCTTGA CCTCCCTAAA 
GGTGAAGAAG TGACCATACT GGCTCAGAAG AAGAAGGACG TTTATCGCCG CATTGTAGAA 
TCAGATGTAG GAGATTCATT CTATATTAGA ACGCATTTTG AATATGAAAA AGAATCTCCT 
TACGGACTTA GTTTTAACAA AGGAGAGGTG TTCCGGGTCG TGGATACTTT ATACAATGGA 
AAGCTGGGCT CTTGGCTTGC CATTCGAATT GGCAAAAATC ATAAGGAGGT AGAACGAGGC 
ATCATCCCTA ATAAGAACAG AGCTGAACAG TTAGCCAGTG TACAGTACAC ACTTCCAAAG 
ACAGCGGGTG GTGATCGGGC AGACTTCTGG AGGTTTCGAG GTCTTCGCAG CTCCAAGAGA 
AATCTTCGAA AAAGCAGAGA GGACTTGTCA GCTCAGCCAG TTCAAACTAA GTTCCCAGCT 
TATGAAAGGG TTGTTCTTCG AGAAGCTGGA TTCCTAAGAC CTGTAACCAT CTTTGGACCA 
ATAGCTGATG TTGCCAGAGA AAAGTTGGCA AGAGAGGAGC CAGATATCTA TCAGATTGCA 
AAAAGTGAAC CACGAGATGC TGGGACTGAC CATCGTAGCT CTGGCATCAT TCGCCTTCAT 
ACAATAAAGC AAATCATAGA TCAGGATAAA CATGCTTTAT TAGATGTAAC GCCAAATGCG 
GTTGATCGTC TTAATTATGC GCAGTGGTAT CCAATTGTTG TGTTCCTTAA CCCTGACTCT 
AAGCAAGGTG TAAAAACAAT GAGGATGAGG TTGTGTCCGG AGTCTCGGAA AAGCGCCAGG 
AAGCTATATG AACGCTCTCA TAAGCTTCGT AAGAACAATC ACCATCTCTT CACAACTACA 
ATTAACTTAA ACTCAATGAA TGATGGTTGG TACGGTGCCC TGAAAGAAGC GATTCAGCAG 
CAACAGAACC AGCTGGTGTG GGTCTCTGAG GGGAAGGCGG ATGGTGCTAC AAGTGATGAC 
CTTGATTTGC ATGACGATCG TCTGTCCTAC CTGTCAGCCC CAGGTAGTGA GTACTCAATG 
TATAGCACGG ACAGTAGACA CACTTCTGAC TATGAAGACA CAGATACAGA AGGCGGGGCC 
TACACTGATC AAGAACTAGA TGAAACTCTT AATGATGAGG TGGGGACTCC CCCGGAGTCT 
GCCATTACAC GGTCCTCTGA GCCTGTAAGA GAGGATTCCT CTGGAATGCA TCATGAAAAC 
CAGACATACC CTCCTTACTC ACCACAAGCG CAGCCACAAG CTATTCATAG AATAGACTCC 
CCTGGACTTA AGCCAGCCTC TCAACAGAAA GCAGAAGCCT CATCTCCAGT CCCTTACCTT 
TCGCCTGAAA CAACCCCAGC ATCATCAGCC TCTGCAGTTA ATCATAATGT CAGTGTAACT 
AATGTCAGCC TGGAGGAGCC TGCCCCAGCC CCTCCCACCT CGCACGCATC ACAGCCTGGT 
TGTTTAGGAG CACCAAGTGC TGAGGCAGCT CACGTAGTGC TCAGAGGTGA AGGACCACCA 
TTGCCGCCGC ATGCAGACCC AGCAAAGGTG TACAGGAAGG AGCCGTATTC TGAAGAAATG 
ATGAGACAAA ACCATATTTT AAAACAACCA GCTCTTGGTC ACCCAGGGCA GAGGCCAGAT 
AAAGAGCCAA ATCTAGCCTA TGAACCCCAA CTTCCATATA TAGAAAAACA AGCCAGCAGA 
GACCTTGAGC AGCCGTCATA CAGGTATGAG GTCTCAAGCT ACACAGACCA GTTTTCTCGG 
AACTATGACC ATCGCCTACG GTTTGAAGAT CGAATCCCTA CCTATGAAGA CCAGTGGTCA 
TATTATGATG ACAAACAGCC CTACCAACCT CGGCCTTTTG AGAATCAGCA TCCCCGAGAC 
CTGGACTCCA GACAACATCC CGAAGAGGCT TCAGAACGAG GTTATTTCCA GCGTTTTGAA 
GAGCCAGCCC CTCTGTCGTA CGACAGTAGA ACACGCTATG AGCAGCTGCC TCGAACCTCT 
ACTCTACGAC ATGAAGAGCA GCCAGCCCCT GCATATGAGG TGCACAACAG GTACAGGCCA 
GAGGCACAGC CCTATTCTTC AACAGGCCCT AAGTCATCTG AGCCCAAGCA GTACTTTGAC 
CAGTACCCGC GAAGTTATGA GCAAGTTCCA CCACCAGGCT TTACCTCCAA AACAGGCCAT 
TACGAGCCTC TCCATGGTGC TGCAGTTGTC CCTCCTCTGA TACCTTCCTC TCAACAAAAG 
CCAGAAGTCC TGCCCTCGGC TACCAAACCA CAGCCTCCAC CCCCAACCCT AACTGAGGAG 
GAGGAGGATC CAGCAATGAA ACCACAGTCT GTGCTCACCA GAGTCAAAAT GTTTGAAAAC 
AAAAGATCTG CGTCTTTGGA GAACAAGAAA GATGTGAATG ACACTGCCAG CTTTAAGCCT 
CCGGAAGTAG CATCTAAACC TCCAGGTGCT TCTCTTGCTG GCCCTAAACC TGTCCCTCAG 
AGTCAGTTTA GTGAGCACGA CAAAACGCTC TACAGGCTCC CAGAGCCTCA GAAACCTCAA 
GTGAAGCCAC CCGAAGATAT TGTTCGATCA AATCATTACG ACCCTGAAGA GGATGAAGAA 
TATTACCGGA AACAGCTCTC CTACTTTGAC CGAAGAAGTT TTGAGAGCAA GCCTTCTGCA 
CATCTTCCTG CTGGCCATCA CTCAGAGCCT GCTAAGCCAG TCCATTCTCA GAGCCAGCCC 
AACTTCTCTA GTTACTCTTC AAAATTTCTT GGCTCTTACA CTAGCTATGA CTACTTGAAA 
AGGAACATCA AGTGGGGAAA ACCCGAAACT GATGCTGTGG ATAGATCATT CAGTGAGAAA 
CGTTATGATC CAGCCCAGGC CACGCCTCCT CCTCCTCCGT TGCCCTCACA GTACAGCCAG 
CCAGCTCCAC CTCTGTCCAG CTCTTCTCTC CACATACATT CCAAGGGCGC CCAGGGTGAA 
GGCAACTCAG TATCATTGGA TTTTCAGAAC TCATATATGT CCAAACCAGA CCCACCCCCA 
TCTCAGAGTA AACCAGCAAC TTTCAGACCA CCAACTCGAG AAGACCCCCC TCAGACTTTC 
TATCCGCAGA AAAGTTTCCC AGACAAAGCT CCAGTTAACG GAGCTGAGCA GACTCAGAAA 
ACCATCACTC CGGTGTACAA TCGATTCACA CCAAAGCCGT ACACAAGTTC TGCCCGGCCA 
TTTGAACGCA AATTTGAAAG TCCGAAGTTC AACCATAATC TTCTGCCAAG TGAAACTGTA 
CATAAACCTG AATTGTCTTC AAAAACTCCC ACTTCCCCAA AAACTCTTAT GAAAGCTCAT 
AGTTCAACAC AGCCTCCAGA GTTTGACAGT GGAGTTGAAA CTTTCTCTGT TCACACAGAT 
AAGCCTAAAT ATCAAATGAA TAATATCAGC ACCATGCCTA AAGCTGTCCC TGTGAGTCCT 
TCAGCTGTGG AAGAAGATGA AGATGAGGAT GGTCATACTG TAGTGGCTAC AGCTCGTGGC 
ATTTTTAACA GCAATGGTGG TGTGTTGAGT TCCATAGAAA CCGGTGTTAG TATAATTATC 
CCACAAGGAG CCATTCCTGA AGGAATTGAG CAAGAAATCT ATTTCAAAGT CTGCAGAGAC 
AATAGCATTC TCCCACCTTT AGATAAAGAG AAAGGTGAAA CTCTGCTGAG CCCCCTAGTG 
ATGTGTGGGC CCCATGGCCT CAAGTTCCTG AAGCCCGTGG AGCTACGCTT GCCACACTGT 
GCGTCCATGA CTCCTGACGG TTGGTCTTTT GCTCTAAAAT CATCCGACTC CTCGTCGGGT 
GACCCTAAAA CCTGGCAAAA CAAGTGTCTT CCTGGAGATC CGAATTACCT TGTTGGAGCC 
AACTGTGTTT CTGTCCTGAT TGACCACTTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030098216.1
CDS	743..6055
Translation	MEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSHPDPEPVSDNEDDSYDEEVHDPRAGRGALANRRSEKSWARDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSQSRSPDQRSEPSDHSTQSPQQPSNGSLRSREEERMSKPGAISTPVKHVDDHPPKAVEEVTVEKNEKQTPTLPEPKPVYAQVGQPDVDLPVSPSDGALPNSAHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDHRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQAIHRIDSPGLKPASQQKAEASSPVPYLSPETTPASSASAVNHNVSVTNVSLEEPAPAPPTSHASQPGCLGAPSAEAAHVVLRGEGPPLPPHADPAKVYRKEPYSEEMMRQNHILKQPALGHPGQRPDKEPNLAYEPQLPYIEKQASRDLEQPSYRYEVSSYTDQFSRNYDHRLRFEDRIPTYEDQWSYYDDKQPYQPRPFENQHPRDLDSRQHPEEASERGYFQRFEEPAPLSYDSRTRYEQLPRTSTLRHEEQPAPAYEVHNRYRPEAQPYSSTGPKSSEPKQYFDQYPRSYEQVPPPGFTSKTGHYEPLHGAAVVPPLIPSSQQKPEVLPSATKPQPPPPTLTEEEEDPAMKPQSVLTRVKMFENKRSASLENKKDVNDTASFKPPEVASKPPGASLAGPKPVPQSQFSEHDKTLYRLPEPQKPQVKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFESKPSAHLPAGHHSEPAKPVHSQSQPNFSSYSSKFLGSYTSYDYLKRNIKWGKPETDAVDRSFSEKRYDPAQATPPPPPLPSQYSQPAPPLSSSSLHIHSKGAQGEGNSVSLDFQNSYMSKPDPPPSQSKPATFRPPTREDPPQTFYPQKSFPDKAPVNGAEQTQKTITPVYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETVHKPELSSKTPTSPKTLMKAHSSTQPPEFDSGVETFSVHTDKPKYQMNNISTMPKAVPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_030242356.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tight junction protein 1 (Tjp1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030242356.1

ORF Insert Sequence:

ATGGAGGAAA CAGCTATATG GGAACAGCAC ACAGTGACGC TTCACAGGGC TCCTGGGTTT 
GGATTTGGAA TTGCAATCTC TGGTGGAAGA GATAATCCTC ATTTTCAGAG TGGGGAAACC 
TCCATAGTGA TTTCTGATGT GTTAAAAGGA GGGCCAGCTG AAGGACAGCT ACAGGAAAAT 
GACCGAGTTG CAATGGTTAA CGGAGTTTCA ATGGATAACG TTGAACATGC TTTTGCTGTT 
CAGCAGCTAA GGAAGAGTGG GAAAAACGCA AAAATTACTA TCCGAAGGAA GAAGAAAGTT 
CAGATCCCTG TAAGTCACCC AGATCCTGAG CCGGTGTCTG ATAATGAAGA CGATAGTTAT 
GACGAAGAAG TGCATGACCC AAGAGCTGGC CGCGGTGCTT TAGCGAACAG AAGGAGCGAG 
AAGAGCTGGG CAAGGGATAG GAGTGCAAGC AGGGAGAGGA GCCTGTCCCC TCGCTCGGAC 
AGGCGGTCCG TGGCCTCCAG TCAGCCCGCA AAGCCCACCA AGGTCACACT GGTGAAGTCT 
CGGAAAAATG AAGAATATGG TCTTCGATTG GCCAGCCATA TATTTGTAAA GGAAATTTCA 
CAAGATAGTT TGGCAGCAAG AGATGGTAAC ATTCAAGAAG GGGATGTTGT CTTGAAGATA 
AATGGTACTG TGACAGAAAA TATGTCATTG ACAGATGCAA AAACACTGAT AGAAAGGTCT 
AAAGGCAAGT TAAAAATGGT AGTGCAAAGA GATGAGCGGG CTACCTTACT GAACGTCCCT 
GACCTTTCGG ATAGTATCCA TTCTGCTAAT GCCTCGGAAA GAGATGACAT TTCAGAAATT 
CAGTCACTAG CATCAGACCA TTCAGGTCGC TCGCATGACA GGCCACCCCG CCGCAGCCAG 
TCACGATCTC CTGACCAACG TTCAGAGCCC TCCGATCATT CCACGCAGTC TCCACAGCAG 
CCCAGCAATG GCAGTCTCCG GAGCAGAGAG GAAGAGCGAA TGTCTAAACC TGGGGCCATC 
TCAACTCCTG TAAAACATGT AGACGATCAT CCACCCAAAG CAGTGGAAGA AGTTACAGTT 
GAGAAAAATG AGAAGCAGAC GCCCACTCTT CCAGAACCGA AACCTGTGTA TGCTCAAGTT 
GGACAACCAG ATGTGGATTT ACCCGTCAGC CCTTCTGATG GTGCTCTGCC TAATTCAGCT 
CATGAAGACG GGATACTTAG GCCCAGCATG AAACTGGTAA AATTCAGAAA AGGAGATAGT 
GTGGGTTTGC GACTAGCTGG TGGAAATGAT GTCGGAATAT TTGTAGCTGG CGTTCTAGAA 
GATAGCCCTG CAGCCAAAGA AGGCTTAGAG GAAGGTGATC AAATTCTCAG GGTGAACAAT 
GTAGATTTCA CAAATATCAT AAGGGAAGAG GCCGTCCTTT TCCTCCTTGA CCTCCCTAAA 
GGTGAAGAAG TGACCATACT GGCTCAGAAG AAGAAGGACG TTTATCGCCG CATTGTAGAA 
TCAGATGTAG GAGATTCATT CTATATTAGA ACGCATTTTG AATATGAAAA AGAATCTCCT 
TACGGACTTA GTTTTAACAA AGGAGAGGTG TTCCGGGTCG TGGATACTTT ATACAATGGA 
AAGCTGGGCT CTTGGCTTGC CATTCGAATT GGCAAAAATC ATAAGGAGGT AGAACGAGGC 
ATCATCCCTA ATAAGAACAG AGCTGAACAG TTAGCCAGTG TACAGTACAC ACTTCCAAAG 
ACAGCGGGTG GTGATCGGGC AGACTTCTGG AGGTTTCGAG GTCTTCGCAG CTCCAAGAGA 
AATCTTCGAA AAAGCAGAGA GGACTTGTCA GCTCAGCCAG TTCAAACTAA GTTCCCAGCT 
TATGAAAGGG TTGTTCTTCG AGAAGCTGGA TTCCTAAGAC CTGTAACCAT CTTTGGACCA 
ATAGCTGATG TTGCCAGAGA AAAGTTGGCA AGAGAGGAGC CAGATATCTA TCAGATTGCA 
AAAAGTGAAC CACGAGATGC TGGGACTGAC CATCGTAGCT CTGGCATCAT TCGCCTTCAT 
ACAATAAAGC AAATCATAGA TCAGGATAAA CATGCTTTAT TAGATGTAAC GCCAAATGCG 
GTTGATCGTC TTAATTATGC GCAGTGGTAT CCAATTGTTG TGTTCCTTAA CCCTGACTCT 
AAGCAAGGTG TAAAAACAAT GAGGATGAGG TTGTGTCCGG AGTCTCGGAA AAGCGCCAGG 
AAGCTATATG AACGCTCTCA TAAGCTTCGT AAGAACAATC ACCATCTCTT CACAACTACA 
ATTAACTTAA ACTCAATGAA TGATGGTTGG TACGGTGCCC TGAAAGAAGC GATTCAGCAG 
CAACAGAACC AGCTGGTGTG GGTCTCTGAG GGGAAGGCGG ATGGTGCTAC AAGTGATGAC 
CTTGATTTGC ATGACGATCG TCTGTCCTAC CTGTCAGCCC CAGGTAGTGA GTACTCAATG 
TATAGCACGG ACAGTAGACA CACTTCTGAC TATGAAGACA CAGATACAGA AGGCGGGGCC 
TACACTGATC AAGAACTAGA TGAAACTCTT AATGATGAGG TGGGGACTCC CCCGGAGTCT 
GCCATTACAC GGTCCTCTGA GCCTGTAAGA GAGGATTCCT CTGGAATGCA TCATGAAAAC 
CAGACATACC CTCCTTACTC ACCACAAGCG CAGCCACAAG CTATTCATAG AATAGACTCC 
CCTGGACTTA AGCCAGCCTC TCAACAGAAA GCAGAAGCCT CATCTCCAGT CCCTTACCTT 
TCGCCTGAAA CAACCCCAGC ATCATCAGCC TCTGCAGTTA ATCATAATGT CAGTGTAACT 
AATGTCAGCC TGGAGGAGCC TGCCCCAGCC CCTCCCACCT CGCACGCATC ACAGCCTGGT 
TGTTTAGGAG CACCAAGTGC TGAGGCAGCT CACGTAGTGC TCAGAGGTGA AGGACCACCA 
TTGCCGCCGC ATGCAGACCC AGCAAAGGTG TACAGGAAGG AGCCGTATTC TGAAGAAATG 
ATGAGACAAA ACCATATTTT AAAACAACCA GCTCTTGGTC ACCCAGGGCA GAGGCCAGAT 
AAAGAGCCAA ATCTAGCCTA TGAACCCCAA CTTCCATATA TAGAAAAACA AGCCAGCAGA 
GACCTTGAGC AGCCGTCATA CAGGTATGAG GTCTCAAGCT ACACAGACCA GTTTTCTCGG 
AACTATGACC ATCGCCTACG GTTTGAAGAT CGAATCCCTA CCTATGAAGA CCAGTGGTCA 
TATTATGATG ACAAACAGCC CTACCAACCT CGGCCTTTTG AGAATCAGCA TCCCCGAGAC 
CTGGACTCCA GACAACATCC CGAAGAGGCT TCAGAACGAG GTTATTTCCA GCGTTTTGAA 
GAGCCAGCCC CTCTGTCGTA CGACAGTAGA ACACGCTATG AGCAGCTGCC TCGAACCTCT 
ACTCTACGAC ATGAAGAGCA GCCAGCCCCT GCATATGAGG TGCACAACAG GTACAGGCCA 
GAGGCACAGC CCTATTCTTC AACAGGCCCT AAGTCATCTG AGCCCAAGCA GTACTTTGAC 
CAGTACCCGC GAAGTTATGA GCAAGTTCCA CCACCAGGCT TTACCTCCAA AACAGGCCAT 
TACGAGCCTC TCCATGGTGC TGCAGTTGTC CCTCCTCTGA TACCTTCCTC TCAACAAAAG 
CCAGAAGTCC TGCCCTCGGC TACCAAACCA CAGCCTCCAC CCCCAACCCT AACTGAGGAG 
GAGGAGGATC CAGCAATGAA ACCACAGTCT GTGCTCACCA GAGTCAAAAT GTTTGAAAAC 
AAAAGATCTG CGTCTTTGGA GAACAAGAAA GATGTGAATG ACACTGCCAG CTTTAAGCCT 
CCGGAAGTAG CATCTAAACC TCCAGGTGCT TCTCTTGCTG GCCCTAAACC TGTCCCTCAG 
AGTCAGTTTA GTGAGCACGA CAAAACGCTC TACAGGCTCC CAGAGCCTCA GAAACCTCAA 
GTGAAGCCAC CCGAAGATAT TGTTCGATCA AATCATTACG ACCCTGAAGA GGATGAAGAA 
TATTACCGGA AACAGCTCTC CTACTTTGAC CGAAGAAGTT TTGAGAGCAA GCCTTCTGCA 
CATCTTCCTG CTGGCCATCA CTCAGAGCCT GCTAAGCCAG TCCATTCTCA GAGCCAGCCC 
AACTTCTCTA GTTACTCTTC AAAATTTCTT GGCTCTTACA CTAGCTATGA CTACTTGAAA 
AGGAACATCA AGTGGGGAAA ACCCGAAACT GATGCTGTGG ATAGATCATT CAGTGAGAAA 
CGTTATGATC CAGCCCAGGC CACGCCTCCT CCTCCTCCGT TGCCCTCACA GTACAGCCAG 
CCAGCTCCAC CTCTGTCCAG CTCTTCTCTC CACATACATT CCAAGGGCGC CCAGGGTGAA 
GGCAACTCAG TATCATTGGA TTTTCAGAAC TCATATATGT CCAAACCAGA CCCACCCCCA 
TCTCAGAGTA AACCAGCAAC TTTCAGACCA CCAACTCGAG AAGACCCCCC TCAGACTTTC 
TATCCGCAGA AAAGTTTCCC AGACAAAGCT CCAGTTAACG GAGCTGAGCA GACTCAGAAA 
ACCATCACTC CGGTGTACAA TCGATTCACA CCAAAGCCGT ACACAAGTTC TGCCCGGCCA 
TTTGAACGCA AATTTGAAAG TCCGAAGTTC AACCATAATC TTCTGCCAAG TGAAACTGTA 
CATAAACCTG AATTGTCTTC AAAAACTCCC ACTTCCCCAA AAACTCTTAT GAAAGCTCAT 
AGTTCAACAC AGCCTCCAGA GTTTGACAGT GGAGTTGAAA CTTTCTCTGT TCACACAGAT 
AAGCCTAAAT ATCAAATGAA TAATATCAGC ACCATGCCTA AAGCTGTCCC TGTGAGTCCT 
TCAGCTGTGG AAGAAGATGA AGATGAGGAT GGTCATACTG TAGTGGCTAC AGCTCGTGGC 
ATTTTTAACA GCAATGGTGG TGTGTTGAGT TCCATAGAAA CCGGTGTTAG TATAATTATC 
CCACAAGGAG CCATTCCTGA AGGAATTGAG CAAGAAATCT ATTTCAAAGT CTGCAGAGAC 
AATAGCATTC TCCCACCTTT AGATAAAGAG AAAGGTGAAA CTCTGCTGAG CCCCCTAGTG 
ATGTGTGGGC CCCATGGCCT CAAGTTCCTG AAGCCCGTGG AGCTACGCTT GCCACACTGT 
GCGTCCATGA CTCCTGACGG TTGGTCTTTT GCTCTAAAAT CATCCGACTC CTCGTCGGGT 
GACCCTAAAA CCTGGCAAAA CAAGTGTCTT CCTGGAGATC CGAATTACCT TGTTGGAGCC 
AACTGTGTTT CTGTCCTGAT TGACCACTTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tjp1 ( NM_009386.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033412.2 Mus musculus tight junction protein 1 (Tjp1), transcript variant 1, mRNA.

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CloneID	OMu20010
Clone ID Related Accession (Same CDS sequence)	NM_009386.2
Accession Version	NM_009386.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5238bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-31
Organism	Mus musculus(house mouse)
Product	tight junction protein ZO-1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC122222.6 and AC131741.4. On Jul 24, 2009 this sequence version replaced NM_009386.1. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGTCCGCCA GGGCCGCGGC CGCTAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAGCACACAG TGACGCTTCA CAGGGCTCCT GGGTTTGGAT TTGGAATTGC AATCTCTGGT 
GGAAGAGATA ATCCTCATTT TCAGAGTGGG GAAACCTCCA TAGTGATTTC TGATGTGTTA 
AAAGGAGGGC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA 
GTTTCAATGG ATAACGTTGA ACATGCTTTT GCTGTTCAGC AGCTAAGGAA GAGTGGGAAA 
AACGCAAAAA TTACTATCCG AAGGAAGAAG AAAGTTCAGA TCCCTGTAAG TCACCCAGAT 
CCTGAGCCGG TGTCTGATAA TGAAGACGAT AGTTATGACG AAGAAGTGCA TGACCCAAGA 
GCTGGCCGCG GTGCTTTAGC GAACAGAAGG AGCGAGAAGA GCTGGGCAAG GGATAGGAGT 
GCAAGCAGGG AGAGGAGCCT GTCCCCTCGC TCGGACAGGC GGTCCGTGGC CTCCAGTCAG 
CCCGCAAAGC CCACCAAGGT CACACTGGTG AAGTCTCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCCA GCCATATATT TGTAAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGTAACATTC AAGAAGGGGA TGTTGTCTTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAAAC ACTGATAGAA AGGTCTAAAG GCAAGTTAAA AATGGTAGTG 
CAAAGAGATG AGCGGGCTAC CTTACTGAAC GTCCCTGACC TTTCGGATAG TATCCATTCT 
GCTAATGCCT CGGAAAGAGA TGACATTTCA GAAATTCAGT CACTAGCATC AGACCATTCA 
GGTCGCTCGC ATGACAGGCC ACCCCGCCGC AGCCAGTCAC GATCTCCTGA CCAACGTTCA 
GAGCCCTCCG ATCATTCCAC GCAGTCTCCA CAGCAGCCCA GCAATGGCAG TCTCCGGAGC 
AGAGAGGAAG AGCGAATGTC TAAACCTGGG GCCATCTCAA CTCCTGTAAA ACATGTAGAC 
GATCATCCAC CCAAAGCAGT GGAAGAAGTT ACAGTTGAGA AAAATGAGAA GCAGACGCCC 
ACTCTTCCAG AACCGAAACC TGTGTATGCT CAAGTTGGAC AACCAGATGT GGATTTACCC 
GTCAGCCCTT CTGATGGTGC TCTGCCTAAT TCAGCTCATG AAGACGGGAT ACTTAGGCCC 
AGCATGAAAC TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGACT AGCTGGTGGA 
AATGATGTCG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAAGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTG AACAATGTAG ATTTCACAAA TATCATAAGG 
GAAGAGGCCG TCCTTTTCCT CCTTGACCTC CCTAAAGGTG AAGAAGTGAC CATACTGGCT 
CAGAAGAAGA AGGACGTTTA TCGCCGCATT GTAGAATCAG ATGTAGGAGA TTCATTCTAT 
ATTAGAACGC ATTTTGAATA TGAAAAAGAA TCTCCTTACG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GGGTCGTGGA TACTTTATAC AATGGAAAGC TGGGCTCTTG GCTTGCCATT 
CGAATTGGCA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAACAGTTAG CCAGTGTACA GTACACACTT CCAAAGACAG CGGGTGGTGA TCGGGCAGAC 
TTCTGGAGGT TTCGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAC 
TTGTCAGCTC AGCCAGTTCA AACTAAGTTC CCAGCTTATG AAAGGGTTGT TCTTCGAGAA 
GCTGGATTCC TAAGACCTGT AACCATCTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
TTGGCAAGAG AGGAGCCAGA TATCTATCAG ATTGCAAAAA GTGAACCACG AGATGCTGGG 
ACTGACCATC GTAGCTCTGG CATCATTCGC CTTCATACAA TAAAGCAAAT CATAGATCAG 
GATAAACATG CTTTATTAGA TGTAACGCCA AATGCGGTTG ATCGTCTTAA TTATGCGCAG 
TGGTATCCAA TTGTTGTGTT CCTTAACCCT GACTCTAAGC AAGGTGTAAA AACAATGAGG 
ATGAGGTTGT GTCCGGAGTC TCGGAAAAGC GCCAGGAAGC TATATGAACG CTCTCATAAG 
CTTCGTAAGA ACAATCACCA TCTCTTCACA ACTACAATTA ACTTAAACTC AATGAATGAT 
GGTTGGTACG GTGCCCTGAA AGAAGCGATT CAGCAGCAAC AGAACCAGCT GGTGTGGGTC 
TCTGAGGGGA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA CGATCGTCTG 
TCCTACCTGT CAGCCCCAGG TAGTGAGTAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA TACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTGGG GACTCCCCCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTAAGAGAGG ATTCCTCTGG AATGCATCAT GAAAACCAGA CATACCCTCC TTACTCACCA 
CAAGCGCAGC CACAAGCTAT TCATAGAATA GACTCCCCTG GACTTAAGCC AGCCTCTCAA 
CAGAAAGCAG AAGCCTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAC CCCAGCATCA 
TCAGCCTCTG CAGTTAATCA TAATGTCAGT GTAACTAATG TCAGCCTGGA GGAGCCTGCC 
CCAGCCCCTC CCACCTCGCA CGCATCACAG CCTGGTTGTT TAGGAGCACC AAGTGCTGAG 
GCAGCTCACG TAGTGCTCAG AGGTGAAGGA CCACCATTGC CGCCGCATGC AGACCCAGCA 
AAGGTGTACA GGAAGGAGCC GTATTCTGAA GAAATGATGA GACAAAACCA TATTTTAAAA 
CAACCAGCTC TTGGTCACCC AGGGCAGAGG CCAGATAAAG AGCCAAATCT AGCCTATGAA 
CCCCAACTTC CATATATAGA AAAACAAGCC AGCAGAGACC TTGAGCAGCC GTCATACAGG 
TATGAGGTCT CAAGCTACAC AGACCAGTTT TCTCGGAACT ATGACCATCG CCTACGGTTT 
GAAGATCGAA TCCCTACCTA TGAAGACCAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CAACCTCGGC CTTTTGAGAA TCAGCATCCC CGAGACCTGG ACTCCAGACA ACATCCCGAA 
GAGGCTTCAG AACGAGGTTA TTTCCAGCGT TTTGAAGAGC CAGCCCCTCT GTCGTACGAC 
AGTAGAACAC GCTATGAGCA GCTGCCTCGA ACCTCTACTC TACGACATGA AGAGCAGCCA 
GCCCCTGCAT ATGAGGTGCA CAACAGGTAC AGGCCAGAGG CACAGCCCTA TTCTTCAACA 
GGCCCTAAGT CATCTGAGCC CAAGCAGTAC TTTGACCAGT ACCCGCGAAG TTATGAGCAA 
GTTCCACCAC CAGGCTTTAC CTCCAAAACA GGCCATTACG AGCCTCTCCA TGGTGCTGCA 
GTTGTCCCTC CTCTGATACC TTCCTCTCAA CAAAAGCCAG AAGTCCTGCC CTCGGCTACC 
AAACCACAGC CTCCACCCCC AACCCTAACT GAGGAGGAGG AGGATCCAGC AATGAAACCA 
CAGTCTGTGC TCACCAGAGT CAAAATGTTT GAAAACAAAA GATCTGCGTC TTTGGAGAAC 
AAGAAAGATG TGAATGACAC TGCCAGCTTT AAGCCTCCGG AAGTAGCATC TAAACCTCCA 
GGTGCTTCTC TTGCTGGCCC TAAACCTGTC CCTCAGAGTC AGTTTAGTGA GCACGACAAA 
ACGCTCTACA GGCTCCCAGA GCCTCAGAAA CCTCAAGTGA AGCCACCCGA AGATATTGTT 
CGATCAAATC ATTACGACCC TGAAGAGGAT GAAGAATATT ACCGGAAACA GCTCTCCTAC 
TTTGACCGAA GAAGTTTTGA GAGCAAGCCT TCTGCACATC TTCCTGCTGG CCATCACTCA 
GAGCCTGCTA AGCCAGTCCA TTCTCAGAGC CAGCCCAACT TCTCTAGTTA CTCTTCAAAG 
GGAAAACCCG AAACTGATGC TGTGGATAGA TCATTCAGTG AGAAACGTTA TGATCCAGCC 
CAGGCCACGC CTCCTCCTCC TCCGTTGCCC TCACAGTACA GCCAGCCAGC TCCACCTCTG 
TCCAGCTCTT CTCTCCACAT ACATTCCAAG GGCGCCCAGG GTGAAGGCAA CTCAGTATCA 
TTGGATTTTC AGAACTCATA TATGTCCAAA CCAGACCCAC CCCCATCTCA GAGTAAACCA 
GCAACTTTCA GACCACCAAC TCGAGAAGAC CCCCCTCAGA CTTTCTATCC GCAGAAAAGT 
TTCCCAGACA AAGCTCCAGT TAACGGAGCT GAGCAGACTC AGAAAACCAT CACTCCGGTG 
TACAATCGAT TCACACCAAA GCCGTACACA AGTTCTGCCC GGCCATTTGA ACGCAAATTT 
GAAAGTCCGA AGTTCAACCA TAATCTTCTG CCAAGTGAAA CTGTACATAA ACCTGAATTG 
TCTTCAAAAA CTCCCACTTC CCCAAAAACT CTTATGAAAG CTCATAGTTC AACACAGCCT 
CCAGAGTTTG ACAGTGGAGT TGAAACTTTC TCTGTTCACA CAGATAAGCC TAAATATCAA 
ATGAATAATA TCAGCACCAT GCCTAAAGCT GTCCCTGTGA GTCCTTCAGC TGTGGAAGAA 
GATGAAGATG AGGATGGTCA TACTGTAGTG GCTACAGCTC GTGGCATTTT TAACAGCAAT 
GGTGGTGTGT TGAGTTCCAT AGAAACCGGT GTTAGTATAA TTATCCCACA AGGAGCCATT 
CCTGAAGGAA TTGAGCAAGA AATCTATTTC AAAGTCTGCA GAGACAATAG CATTCTCCCA 
CCTTTAGATA AAGAGAAAGG TGAAACTCTG CTGAGCCCCC TAGTGATGTG TGGGCCCCAT 
GGCCTCAAGT TCCTGAAGCC CGTGGAGCTA CGCTTGCCAC ACTGTGACCC TAAAACCTGG 
CAAAACAAGT GTCTTCCTGG AGATCCGAAT TACCTTGTTG GAGCCAACTG TGTTTCTGTC 
CTGATTGACC ACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033412.2
CDS	409..5646
Translation	MSARAAAAKSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSHPDPEPVSDNEDDSYDEEVHDPRAGRGALANRRSEKSWARDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSQSRSPDQRSEPSDHSTQSPQQPSNGSLRSREEERMSKPGAISTPVKHVDDHPPKAVEEVTVEKNEKQTPTLPEPKPVYAQVGQPDVDLPVSPSDGALPNSAHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDHRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQAIHRIDSPGLKPASQQKAEASSPVPYLSPETTPASSASAVNHNVSVTNVSLEEPAPAPPTSHASQPGCLGAPSAEAAHVVLRGEGPPLPPHADPAKVYRKEPYSEEMMRQNHILKQPALGHPGQRPDKEPNLAYEPQLPYIEKQASRDLEQPSYRYEVSSYTDQFSRNYDHRLRFEDRIPTYEDQWSYYDDKQPYQPRPFENQHPRDLDSRQHPEEASERGYFQRFEEPAPLSYDSRTRYEQLPRTSTLRHEEQPAPAYEVHNRYRPEAQPYSSTGPKSSEPKQYFDQYPRSYEQVPPPGFTSKTGHYEPLHGAAVVPPLIPSSQQKPEVLPSATKPQPPPPTLTEEEEDPAMKPQSVLTRVKMFENKRSASLENKKDVNDTASFKPPEVASKPPGASLAGPKPVPQSQFSEHDKTLYRLPEPQKPQVKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFESKPSAHLPAGHHSEPAKPVHSQSQPNFSSYSSKGKPETDAVDRSFSEKRYDPAQATPPPPPLPSQYSQPAPPLSSSSLHIHSKGAQGEGNSVSLDFQNSYMSKPDPPPSQSKPATFRPPTREDPPQTFYPQKSFPDKAPVNGAEQTQKTITPVYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETVHKPELSSKTPTSPKTLMKAHSSTQPPEFDSGVETFSVHTDKPKYQMNNISTMPKAVPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	NM_009386.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus tight junction protein 1 (Tjp1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009386.2

ORF Insert Sequence:

ATGTCCGCCA GGGCCGCGGC CGCTAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAGCACACAG TGACGCTTCA CAGGGCTCCT GGGTTTGGAT TTGGAATTGC AATCTCTGGT 
GGAAGAGATA ATCCTCATTT TCAGAGTGGG GAAACCTCCA TAGTGATTTC TGATGTGTTA 
AAAGGAGGGC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA 
GTTTCAATGG ATAACGTTGA ACATGCTTTT GCTGTTCAGC AGCTAAGGAA GAGTGGGAAA 
AACGCAAAAA TTACTATCCG AAGGAAGAAG AAAGTTCAGA TCCCTGTAAG TCACCCAGAT 
CCTGAGCCGG TGTCTGATAA TGAAGACGAT AGTTATGACG AAGAAGTGCA TGACCCAAGA 
GCTGGCCGCG GTGCTTTAGC GAACAGAAGG AGCGAGAAGA GCTGGGCAAG GGATAGGAGT 
GCAAGCAGGG AGAGGAGCCT GTCCCCTCGC TCGGACAGGC GGTCCGTGGC CTCCAGTCAG 
CCCGCAAAGC CCACCAAGGT CACACTGGTG AAGTCTCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCCA GCCATATATT TGTAAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGTAACATTC AAGAAGGGGA TGTTGTCTTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAAAC ACTGATAGAA AGGTCTAAAG GCAAGTTAAA AATGGTAGTG 
CAAAGAGATG AGCGGGCTAC CTTACTGAAC GTCCCTGACC TTTCGGATAG TATCCATTCT 
GCTAATGCCT CGGAAAGAGA TGACATTTCA GAAATTCAGT CACTAGCATC AGACCATTCA 
GGTCGCTCGC ATGACAGGCC ACCCCGCCGC AGCCAGTCAC GATCTCCTGA CCAACGTTCA 
GAGCCCTCCG ATCATTCCAC GCAGTCTCCA CAGCAGCCCA GCAATGGCAG TCTCCGGAGC 
AGAGAGGAAG AGCGAATGTC TAAACCTGGG GCCATCTCAA CTCCTGTAAA ACATGTAGAC 
GATCATCCAC CCAAAGCAGT GGAAGAAGTT ACAGTTGAGA AAAATGAGAA GCAGACGCCC 
ACTCTTCCAG AACCGAAACC TGTGTATGCT CAAGTTGGAC AACCAGATGT GGATTTACCC 
GTCAGCCCTT CTGATGGTGC TCTGCCTAAT TCAGCTCATG AAGACGGGAT ACTTAGGCCC 
AGCATGAAAC TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGACT AGCTGGTGGA 
AATGATGTCG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAAGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTG AACAATGTAG ATTTCACAAA TATCATAAGG 
GAAGAGGCCG TCCTTTTCCT CCTTGACCTC CCTAAAGGTG AAGAAGTGAC CATACTGGCT 
CAGAAGAAGA AGGACGTTTA TCGCCGCATT GTAGAATCAG ATGTAGGAGA TTCATTCTAT 
ATTAGAACGC ATTTTGAATA TGAAAAAGAA TCTCCTTACG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GGGTCGTGGA TACTTTATAC AATGGAAAGC TGGGCTCTTG GCTTGCCATT 
CGAATTGGCA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAACAGTTAG CCAGTGTACA GTACACACTT CCAAAGACAG CGGGTGGTGA TCGGGCAGAC 
TTCTGGAGGT TTCGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAC 
TTGTCAGCTC AGCCAGTTCA AACTAAGTTC CCAGCTTATG AAAGGGTTGT TCTTCGAGAA 
GCTGGATTCC TAAGACCTGT AACCATCTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
TTGGCAAGAG AGGAGCCAGA TATCTATCAG ATTGCAAAAA GTGAACCACG AGATGCTGGG 
ACTGACCATC GTAGCTCTGG CATCATTCGC CTTCATACAA TAAAGCAAAT CATAGATCAG 
GATAAACATG CTTTATTAGA TGTAACGCCA AATGCGGTTG ATCGTCTTAA TTATGCGCAG 
TGGTATCCAA TTGTTGTGTT CCTTAACCCT GACTCTAAGC AAGGTGTAAA AACAATGAGG 
ATGAGGTTGT GTCCGGAGTC TCGGAAAAGC GCCAGGAAGC TATATGAACG CTCTCATAAG 
CTTCGTAAGA ACAATCACCA TCTCTTCACA ACTACAATTA ACTTAAACTC AATGAATGAT 
GGTTGGTACG GTGCCCTGAA AGAAGCGATT CAGCAGCAAC AGAACCAGCT GGTGTGGGTC 
TCTGAGGGGA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA CGATCGTCTG 
TCCTACCTGT CAGCCCCAGG TAGTGAGTAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA TACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTGGG GACTCCCCCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTAAGAGAGG ATTCCTCTGG AATGCATCAT GAAAACCAGA CATACCCTCC TTACTCACCA 
CAAGCGCAGC CACAAGCTAT TCATAGAATA GACTCCCCTG GACTTAAGCC AGCCTCTCAA 
CAGAAAGCAG AAGCCTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAC CCCAGCATCA 
TCAGCCTCTG CAGTTAATCA TAATGTCAGT GTAACTAATG TCAGCCTGGA GGAGCCTGCC 
CCAGCCCCTC CCACCTCGCA CGCATCACAG CCTGGTTGTT TAGGAGCACC AAGTGCTGAG 
GCAGCTCACG TAGTGCTCAG AGGTGAAGGA CCACCATTGC CGCCGCATGC AGACCCAGCA 
AAGGTGTACA GGAAGGAGCC GTATTCTGAA GAAATGATGA GACAAAACCA TATTTTAAAA 
CAACCAGCTC TTGGTCACCC AGGGCAGAGG CCAGATAAAG AGCCAAATCT AGCCTATGAA 
CCCCAACTTC CATATATAGA AAAACAAGCC AGCAGAGACC TTGAGCAGCC GTCATACAGG 
TATGAGGTCT CAAGCTACAC AGACCAGTTT TCTCGGAACT ATGACCATCG CCTACGGTTT 
GAAGATCGAA TCCCTACCTA TGAAGACCAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CAACCTCGGC CTTTTGAGAA TCAGCATCCC CGAGACCTGG ACTCCAGACA ACATCCCGAA 
GAGGCTTCAG AACGAGGTTA TTTCCAGCGT TTTGAAGAGC CAGCCCCTCT GTCGTACGAC 
AGTAGAACAC GCTATGAGCA GCTGCCTCGA ACCTCTACTC TACGACATGA AGAGCAGCCA 
GCCCCTGCAT ATGAGGTGCA CAACAGGTAC AGGCCAGAGG CACAGCCCTA TTCTTCAACA 
GGCCCTAAGT CATCTGAGCC CAAGCAGTAC TTTGACCAGT ACCCGCGAAG TTATGAGCAA 
GTTCCACCAC CAGGCTTTAC CTCCAAAACA GGCCATTACG AGCCTCTCCA TGGTGCTGCA 
GTTGTCCCTC CTCTGATACC TTCCTCTCAA CAAAAGCCAG AAGTCCTGCC CTCGGCTACC 
AAACCACAGC CTCCACCCCC AACCCTAACT GAGGAGGAGG AGGATCCAGC AATGAAACCA 
CAGTCTGTGC TCACCAGAGT CAAAATGTTT GAAAACAAAA GATCTGCGTC TTTGGAGAAC 
AAGAAAGATG TGAATGACAC TGCCAGCTTT AAGCCTCCGG AAGTAGCATC TAAACCTCCA 
GGTGCTTCTC TTGCTGGCCC TAAACCTGTC CCTCAGAGTC AGTTTAGTGA GCACGACAAA 
ACGCTCTACA GGCTCCCAGA GCCTCAGAAA CCTCAAGTGA AGCCACCCGA AGATATTGTT 
CGATCAAATC ATTACGACCC TGAAGAGGAT GAAGAATATT ACCGGAAACA GCTCTCCTAC 
TTTGACCGAA GAAGTTTTGA GAGCAAGCCT TCTGCACATC TTCCTGCTGG CCATCACTCA 
GAGCCTGCTA AGCCAGTCCA TTCTCAGAGC CAGCCCAACT TCTCTAGTTA CTCTTCAAAG 
GGAAAACCCG AAACTGATGC TGTGGATAGA TCATTCAGTG AGAAACGTTA TGATCCAGCC 
CAGGCCACGC CTCCTCCTCC TCCGTTGCCC TCACAGTACA GCCAGCCAGC TCCACCTCTG 
TCCAGCTCTT CTCTCCACAT ACATTCCAAG GGCGCCCAGG GTGAAGGCAA CTCAGTATCA 
TTGGATTTTC AGAACTCATA TATGTCCAAA CCAGACCCAC CCCCATCTCA GAGTAAACCA 
GCAACTTTCA GACCACCAAC TCGAGAAGAC CCCCCTCAGA CTTTCTATCC GCAGAAAAGT 
TTCCCAGACA AAGCTCCAGT TAACGGAGCT GAGCAGACTC AGAAAACCAT CACTCCGGTG 
TACAATCGAT TCACACCAAA GCCGTACACA AGTTCTGCCC GGCCATTTGA ACGCAAATTT 
GAAAGTCCGA AGTTCAACCA TAATCTTCTG CCAAGTGAAA CTGTACATAA ACCTGAATTG 
TCTTCAAAAA CTCCCACTTC CCCAAAAACT CTTATGAAAG CTCATAGTTC AACACAGCCT 
CCAGAGTTTG ACAGTGGAGT TGAAACTTTC TCTGTTCACA CAGATAAGCC TAAATATCAA 
ATGAATAATA TCAGCACCAT GCCTAAAGCT GTCCCTGTGA GTCCTTCAGC TGTGGAAGAA 
GATGAAGATG AGGATGGTCA TACTGTAGTG GCTACAGCTC GTGGCATTTT TAACAGCAAT 
GGTGGTGTGT TGAGTTCCAT AGAAACCGGT GTTAGTATAA TTATCCCACA AGGAGCCATT 
CCTGAAGGAA TTGAGCAAGA AATCTATTTC AAAGTCTGCA GAGACAATAG CATTCTCCCA 
CCTTTAGATA AAGAGAAAGG TGAAACTCTG CTGAGCCCCC TAGTGATGTG TGGGCCCCAT 
GGCCTCAAGT TCCTGAAGCC CGTGGAGCTA CGCTTGCCAC ACTGTGACCC TAAAACCTGG 
CAAAACAAGT GTCTTCCTGG AGATCCGAAT TACCTTGTTG GAGCCAACTG TGTTTCTGTC 
CTGATTGACC ACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tjp1 ( NM_001163574.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001157046.1 Mus musculus tight junction protein 1 (Tjp1), transcript variant 2, mRNA.

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CloneID	OMu22535
Clone ID Related Accession (Same CDS sequence)	NM_001163574.1
Accession Version	NM_001163574.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-31
Organism	Mus musculus(house mouse)
Product	tight junction protein ZO-1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ098724.1, BC138028.1 and BP760988.1. Transcript Variant: This variant (2) lacks an in-frame coding exon in the middle region but has an additional in-frame segment in the 3' coding region, as compared to variant 1. The resulting isoform (2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC138028.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGTCCGCCA GGGCCGCGGC CGCTAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAGCACACAG TGACGCTTCA CAGGGCTCCT GGGTTTGGAT TTGGAATTGC AATCTCTGGT 
GGAAGAGATA ATCCTCATTT TCAGAGTGGG GAAACCTCCA TAGTGATTTC TGATGTGTTA 
AAAGGAGGGC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA 
GTTTCAATGG ATAACGTTGA ACATGCTTTT GCTGTTCAGC AGCTAAGGAA GAGTGGGAAA 
AACGCAAAAA TTACTATCCG AAGGAAGAAG AAAGTTCAGA TCCCTGTAAG TCACCCAGAT 
CCTGAGCCGG TGTCTGATAA TGAAGACGAT AGTTATGACG AAGAAGTGCA TGACCCAAGA 
GCTGGCCGCG GTGCTTTAGC GAACAGAAGG AGCGAGAAGA GCTGGGCAAG GGATAGGAGT 
GCAAGCAGGG AGAGGAGCCT GTCCCCTCGC TCGGACAGGC GGTCCGTGGC CTCCAGTCAG 
CCCGCAAAGC CCACCAAGGT CACACTGGTG AAGTCTCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCCA GCCATATATT TGTAAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGTAACATTC AAGAAGGGGA TGTTGTCTTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAAAC ACTGATAGAA AGGTCTAAAG GCAAGTTAAA AATGGTAGTG 
CAAAGAGATG AGCGGGCTAC CTTACTGAAC GTCCCTGACC TTTCGGATAG TATCCATTCT 
GCTAATGCCT CGGAAAGAGA TGACATTTCA GAAATTCAGT CACTAGCATC AGACCATTCA 
GGTCGCTCGC ATGACAGGCC ACCCCGCCGC AGCCAGTCAC GATCTCCTGA CCAACGTTCA 
GAGCCCTCCG ATCATTCCAC GCAGTCTCCA CAGCAGCCCA GCAATGGCAG TCTCCGGAGC 
AGAGAGGAAG AGCGAATGTC TAAACCTGGG GCCATCTCAA CTCCTGTAAA ACATGTAGAC 
GATCATCCAC CCAAAGCAGT GGAAGAAGTT ACAGTTGAGA AAAATGAGAA GCAGACGCCC 
ACTCTTCCAG AACCGAAACC TGTGTATGCT CAAGTTGGAC AACCAGATGT GGATTTACCC 
GTCAGCCCTT CTGATGGTGC TCTGCCTAAT TCAGCTCATG AAGACGGGAT ACTTAGGCCC 
AGCATGAAAC TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGACT AGCTGGTGGA 
AATGATGTCG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAAGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTG AACAATGTAG ATTTCACAAA TATCATAAGG 
GAAGAGGCCG TCCTTTTCCT CCTTGACCTC CCTAAAGGTG AAGAAGTGAC CATACTGGCT 
CAGAAGAAGA AGGACGTTTA TCGCCGCATT GTAGAATCAG ATGTAGGAGA TTCATTCTAT 
ATTAGAACGC ATTTTGAATA TGAAAAAGAA TCTCCTTACG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GGGTCGTGGA TACTTTATAC AATGGAAAGC TGGGCTCTTG GCTTGCCATT 
CGAATTGGCA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAACAGTTAG CCAGTGTACA GTACACACTT CCAAAGACAG CGGGTGGTGA TCGGGCAGAC 
TTCTGGAGGT TTCGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAC 
TTGTCAGCTC AGCCAGTTCA AACTAAGTTC CCAGCTTATG AAAGGGTTGT TCTTCGAGAA 
GCTGGATTCC TAAGACCTGT AACCATCTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
TTGGCAAGAG AGGAGCCAGA TATCTATCAG ATTGCAAAAA GTGAACCACG AGATGCTGGG 
ACTGACCATC GTAGCTCTGG CATCATTCGC CTTCATACAA TAAAGCAAAT CATAGATCAG 
GATAAACATG CTTTATTAGA TGTAACGCCA AATGCGGTTG ATCGTCTTAA TTATGCGCAG 
TGGTATCCAA TTGTTGTGTT CCTTAACCCT GACTCTAAGC AAGGTGTAAA AACAATGAGG 
ATGAGGTTGT GTCCGGAGTC TCGGAAAAGC GCCAGGAAGC TATATGAACG CTCTCATAAG 
CTTCGTAAGA ACAATCACCA TCTCTTCACA ACTACAATTA ACTTAAACTC AATGAATGAT 
GGTTGGTACG GTGCCCTGAA AGAAGCGATT CAGCAGCAAC AGAACCAGCT GGTGTGGGTC 
TCTGAGGGGA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA CGATCGTCTG 
TCCTACCTGT CAGCCCCAGG TAGTGAGTAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA TACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTGGG GACTCCCCCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTAAGAGAGG ATTCCTCTGG AATGCATCAT GAAAACCAGA CATACCCTCC TTACTCACCA 
CAAGCGCAGC CACAAGCTAT TCATAGAATA GACTCCCCTG GACTTAAGCC AGCCTCTCAA 
CAGGTGTACA GGAAGGAGCC GTATTCTGAA GAAATGATGA GACAAAACCA TATTTTAAAA 
CAACCAGCTC TTGGTCACCC AGGGCAGAGG CCAGATAAAG AGCCAAATCT AGCCTATGAA 
CCCCAACTTC CATATATAGA AAAACAAGCC AGCAGAGACC TTGAGCAGCC GTCATACAGG 
TATGAGGTCT CAAGCTACAC AGACCAGTTT TCTCGGAACT ATGACCATCG CCTACGGTTT 
GAAGATCGAA TCCCTACCTA TGAAGACCAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CAACCTCGGC CTTTTGAGAA TCAGCATCCC CGAGACCTGG ACTCCAGACA ACATCCCGAA 
GAGGCTTCAG AACGAGGTTA TTTCCAGCGT TTTGAAGAGC CAGCCCCTCT GTCGTACGAC 
AGTAGAACAC GCTATGAGCA GCTGCCTCGA ACCTCTACTC TACGACATGA AGAGCAGCCA 
GCCCCTGCAT ATGAGGTGCA CAACAGGTAC AGGCCAGAGG CACAGCCCTA TTCTTCAACA 
GGCCCTAAGT CATCTGAGCC CAAGCAGTAC TTTGACCAGT ACCCGCGAAG TTATGAGCAA 
GTTCCACCAC CAGGCTTTAC CTCCAAAACA GGCCATTACG AGCCTCTCCA TGGTGCTGCA 
GTTGTCCCTC CTCTGATACC TTCCTCTCAA CAAAAGCCAG AAGTCCTGCC CTCGGCTACC 
AAACCACAGC CTCCACCCCC AACCCTAACT GAGGAGGAGG AGGATCCAGC AATGAAACCA 
CAGTCTGTGC TCACCAGAGT CAAAATGTTT GAAAACAAAA GATCTGCGTC TTTGGAGAAC 
AAGAAAGATG TGAATGACAC TGCCAGCTTT AAGCCTCCGG AAGTAGCATC TAAACCTCCA 
GGTGCTTCTC TTGCTGGCCC TAAACCTGTC CCTCAGAGTC AGTTTAGTGA GCACGACAAA 
ACGCTCTACA GGCTCCCAGA GCCTCAGAAA CCTCAAGTGA AGCCACCCGA AGATATTGTT 
CGATCAAATC ATTACGACCC TGAAGAGGAT GAAGAATATT ACCGGAAACA GCTCTCCTAC 
TTTGACCGAA GAAGTTTTGA GAGCAAGCCT TCTGCACATC TTCCTGCTGG CCATCACTCA 
GAGCCTGCTA AGCCAGTCCA TTCTCAGAGC CAGCCCAACT TCTCTAGTTA CTCTTCAAAG 
GGAAAACCCG AAACTGATGC TGTGGATAGA TCATTCAGTG AGAAACGTTA TGATCCAGCC 
CAGGCCACGC CTCCTCCTCC TCCGTTGCCC TCACAGTACA GCCAGCCAGC TCCACCTCTG 
TCCAGCTCTT CTCTCCACAT ACATTCCAAG GGCGCCCAGG GTGAAGGCAA CTCAGTATCA 
TTGGATTTTC AGAACTCATA TATGTCCAAA CCAGACCCAC CCCCATCTCA GAGTAAACCA 
GCAACTTTCA GACCACCAAC TCGAGAAGAC CCCCCTCAGA CTTTCTATCC GCAGAAAAGT 
TTCCCAGACA AAGCTCCAGT TAACGGAGCT GAGCAGACTC AGAAAACCAT CACTCCGGTG 
TACAATCGAT TCACACCAAA GCCGTACACA AGTTCTGCCC GGCCATTTGA ACGCAAATTT 
GAAAGTCCGA AGTTCAACCA TAATCTTCTG CCAAGTGAAA CTGTACATAA ACCTGAATTG 
TCTTCAAAAA CTCCCACTTC CCCAAAAACT CTTATGAAAG CTCATAGTTC AACACAGCCT 
CCAGAGTTTG ACAGTGGAGT TGAAACTTTC TCTGTTCACA CAGATAAGCC TAAATATCAA 
ATGAATAATA TCAGCACCAT GCCTAAAGCT GTCCCTGTGA GTCCTTCAGC TGTGGAAGAA 
GATGAAGATG AGGATGGTCA TACTGTAGTG GCTACAGCTC GTGGCATTTT TAACAGCAAT 
GGTGGTGTGT TGAGTTCCAT AGAAACCGGT GTTAGTATAA TTATCCCACA AGGAGCCATT 
CCTGAAGGAA TTGAGCAAGA AATCTATTTC AAAGTCTGCA GAGACAATAG CATTCTCCCA 
CCTTTAGATA AAGAGAAAGG TGAAACTCTG CTGAGCCCCC TAGTGATGTG TGGGCCCCAT 
GGCCTCAAGT TCCTGAAGCC CGTGGAGCTA CGCTTGCCAC ACTGTGCGTC CATGACTCCT 
GACGGTTGGT CTTTTGCTCT AAAATCATCC GACTCCTCGT CGGGTGACCC TAAAACCTGG 
CAAAACAAGT GTCTTCCTGG AGATCCGAAT TACCTTGTTG GAGCCAACTG TGTTTCTGTC 
CTGATTGACC ACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001157046.1
CDS	409..5466
Translation	MSARAAAAKSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSHPDPEPVSDNEDDSYDEEVHDPRAGRGALANRRSEKSWARDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSQSRSPDQRSEPSDHSTQSPQQPSNGSLRSREEERMSKPGAISTPVKHVDDHPPKAVEEVTVEKNEKQTPTLPEPKPVYAQVGQPDVDLPVSPSDGALPNSAHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDHRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQAIHRIDSPGLKPASQQVYRKEPYSEEMMRQNHILKQPALGHPGQRPDKEPNLAYEPQLPYIEKQASRDLEQPSYRYEVSSYTDQFSRNYDHRLRFEDRIPTYEDQWSYYDDKQPYQPRPFENQHPRDLDSRQHPEEASERGYFQRFEEPAPLSYDSRTRYEQLPRTSTLRHEEQPAPAYEVHNRYRPEAQPYSSTGPKSSEPKQYFDQYPRSYEQVPPPGFTSKTGHYEPLHGAAVVPPLIPSSQQKPEVLPSATKPQPPPPTLTEEEEDPAMKPQSVLTRVKMFENKRSASLENKKDVNDTASFKPPEVASKPPGASLAGPKPVPQSQFSEHDKTLYRLPEPQKPQVKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFESKPSAHLPAGHHSEPAKPVHSQSQPNFSSYSSKGKPETDAVDRSFSEKRYDPAQATPPPPPLPSQYSQPAPPLSSSSLHIHSKGAQGEGNSVSLDFQNSYMSKPDPPPSQSKPATFRPPTREDPPQTFYPQKSFPDKAPVNGAEQTQKTITPVYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETVHKPELSSKTPTSPKTLMKAHSSTQPPEFDSGVETFSVHTDKPKYQMNNISTMPKAVPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	NM_001163574.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tight junction protein 1 (Tjp1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001163574.1

ORF Insert Sequence:

ATGTCCGCCA GGGCCGCGGC CGCTAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAGCACACAG TGACGCTTCA CAGGGCTCCT GGGTTTGGAT TTGGAATTGC AATCTCTGGT 
GGAAGAGATA ATCCTCATTT TCAGAGTGGG GAAACCTCCA TAGTGATTTC TGATGTGTTA 
AAAGGAGGGC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA 
GTTTCAATGG ATAACGTTGA ACATGCTTTT GCTGTTCAGC AGCTAAGGAA GAGTGGGAAA 
AACGCAAAAA TTACTATCCG AAGGAAGAAG AAAGTTCAGA TCCCTGTAAG TCACCCAGAT 
CCTGAGCCGG TGTCTGATAA TGAAGACGAT AGTTATGACG AAGAAGTGCA TGACCCAAGA 
GCTGGCCGCG GTGCTTTAGC GAACAGAAGG AGCGAGAAGA GCTGGGCAAG GGATAGGAGT 
GCAAGCAGGG AGAGGAGCCT GTCCCCTCGC TCGGACAGGC GGTCCGTGGC CTCCAGTCAG 
CCCGCAAAGC CCACCAAGGT CACACTGGTG AAGTCTCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCCA GCCATATATT TGTAAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGTAACATTC AAGAAGGGGA TGTTGTCTTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAAAC ACTGATAGAA AGGTCTAAAG GCAAGTTAAA AATGGTAGTG 
CAAAGAGATG AGCGGGCTAC CTTACTGAAC GTCCCTGACC TTTCGGATAG TATCCATTCT 
GCTAATGCCT CGGAAAGAGA TGACATTTCA GAAATTCAGT CACTAGCATC AGACCATTCA 
GGTCGCTCGC ATGACAGGCC ACCCCGCCGC AGCCAGTCAC GATCTCCTGA CCAACGTTCA 
GAGCCCTCCG ATCATTCCAC GCAGTCTCCA CAGCAGCCCA GCAATGGCAG TCTCCGGAGC 
AGAGAGGAAG AGCGAATGTC TAAACCTGGG GCCATCTCAA CTCCTGTAAA ACATGTAGAC 
GATCATCCAC CCAAAGCAGT GGAAGAAGTT ACAGTTGAGA AAAATGAGAA GCAGACGCCC 
ACTCTTCCAG AACCGAAACC TGTGTATGCT CAAGTTGGAC AACCAGATGT GGATTTACCC 
GTCAGCCCTT CTGATGGTGC TCTGCCTAAT TCAGCTCATG AAGACGGGAT ACTTAGGCCC 
AGCATGAAAC TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGACT AGCTGGTGGA 
AATGATGTCG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAAGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTG AACAATGTAG ATTTCACAAA TATCATAAGG 
GAAGAGGCCG TCCTTTTCCT CCTTGACCTC CCTAAAGGTG AAGAAGTGAC CATACTGGCT 
CAGAAGAAGA AGGACGTTTA TCGCCGCATT GTAGAATCAG ATGTAGGAGA TTCATTCTAT 
ATTAGAACGC ATTTTGAATA TGAAAAAGAA TCTCCTTACG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GGGTCGTGGA TACTTTATAC AATGGAAAGC TGGGCTCTTG GCTTGCCATT 
CGAATTGGCA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAACAGTTAG CCAGTGTACA GTACACACTT CCAAAGACAG CGGGTGGTGA TCGGGCAGAC 
TTCTGGAGGT TTCGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAC 
TTGTCAGCTC AGCCAGTTCA AACTAAGTTC CCAGCTTATG AAAGGGTTGT TCTTCGAGAA 
GCTGGATTCC TAAGACCTGT AACCATCTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
TTGGCAAGAG AGGAGCCAGA TATCTATCAG ATTGCAAAAA GTGAACCACG AGATGCTGGG 
ACTGACCATC GTAGCTCTGG CATCATTCGC CTTCATACAA TAAAGCAAAT CATAGATCAG 
GATAAACATG CTTTATTAGA TGTAACGCCA AATGCGGTTG ATCGTCTTAA TTATGCGCAG 
TGGTATCCAA TTGTTGTGTT CCTTAACCCT GACTCTAAGC AAGGTGTAAA AACAATGAGG 
ATGAGGTTGT GTCCGGAGTC TCGGAAAAGC GCCAGGAAGC TATATGAACG CTCTCATAAG 
CTTCGTAAGA ACAATCACCA TCTCTTCACA ACTACAATTA ACTTAAACTC AATGAATGAT 
GGTTGGTACG GTGCCCTGAA AGAAGCGATT CAGCAGCAAC AGAACCAGCT GGTGTGGGTC 
TCTGAGGGGA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA CGATCGTCTG 
TCCTACCTGT CAGCCCCAGG TAGTGAGTAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA TACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTGGG GACTCCCCCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTAAGAGAGG ATTCCTCTGG AATGCATCAT GAAAACCAGA CATACCCTCC TTACTCACCA 
CAAGCGCAGC CACAAGCTAT TCATAGAATA GACTCCCCTG GACTTAAGCC AGCCTCTCAA 
CAGGTGTACA GGAAGGAGCC GTATTCTGAA GAAATGATGA GACAAAACCA TATTTTAAAA 
CAACCAGCTC TTGGTCACCC AGGGCAGAGG CCAGATAAAG AGCCAAATCT AGCCTATGAA 
CCCCAACTTC CATATATAGA AAAACAAGCC AGCAGAGACC TTGAGCAGCC GTCATACAGG 
TATGAGGTCT CAAGCTACAC AGACCAGTTT TCTCGGAACT ATGACCATCG CCTACGGTTT 
GAAGATCGAA TCCCTACCTA TGAAGACCAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CAACCTCGGC CTTTTGAGAA TCAGCATCCC CGAGACCTGG ACTCCAGACA ACATCCCGAA 
GAGGCTTCAG AACGAGGTTA TTTCCAGCGT TTTGAAGAGC CAGCCCCTCT GTCGTACGAC 
AGTAGAACAC GCTATGAGCA GCTGCCTCGA ACCTCTACTC TACGACATGA AGAGCAGCCA 
GCCCCTGCAT ATGAGGTGCA CAACAGGTAC AGGCCAGAGG CACAGCCCTA TTCTTCAACA 
GGCCCTAAGT CATCTGAGCC CAAGCAGTAC TTTGACCAGT ACCCGCGAAG TTATGAGCAA 
GTTCCACCAC CAGGCTTTAC CTCCAAAACA GGCCATTACG AGCCTCTCCA TGGTGCTGCA 
GTTGTCCCTC CTCTGATACC TTCCTCTCAA CAAAAGCCAG AAGTCCTGCC CTCGGCTACC 
AAACCACAGC CTCCACCCCC AACCCTAACT GAGGAGGAGG AGGATCCAGC AATGAAACCA 
CAGTCTGTGC TCACCAGAGT CAAAATGTTT GAAAACAAAA GATCTGCGTC TTTGGAGAAC 
AAGAAAGATG TGAATGACAC TGCCAGCTTT AAGCCTCCGG AAGTAGCATC TAAACCTCCA 
GGTGCTTCTC TTGCTGGCCC TAAACCTGTC CCTCAGAGTC AGTTTAGTGA GCACGACAAA 
ACGCTCTACA GGCTCCCAGA GCCTCAGAAA CCTCAAGTGA AGCCACCCGA AGATATTGTT 
CGATCAAATC ATTACGACCC TGAAGAGGAT GAAGAATATT ACCGGAAACA GCTCTCCTAC 
TTTGACCGAA GAAGTTTTGA GAGCAAGCCT TCTGCACATC TTCCTGCTGG CCATCACTCA 
GAGCCTGCTA AGCCAGTCCA TTCTCAGAGC CAGCCCAACT TCTCTAGTTA CTCTTCAAAG 
GGAAAACCCG AAACTGATGC TGTGGATAGA TCATTCAGTG AGAAACGTTA TGATCCAGCC 
CAGGCCACGC CTCCTCCTCC TCCGTTGCCC TCACAGTACA GCCAGCCAGC TCCACCTCTG 
TCCAGCTCTT CTCTCCACAT ACATTCCAAG GGCGCCCAGG GTGAAGGCAA CTCAGTATCA 
TTGGATTTTC AGAACTCATA TATGTCCAAA CCAGACCCAC CCCCATCTCA GAGTAAACCA 
GCAACTTTCA GACCACCAAC TCGAGAAGAC CCCCCTCAGA CTTTCTATCC GCAGAAAAGT 
TTCCCAGACA AAGCTCCAGT TAACGGAGCT GAGCAGACTC AGAAAACCAT CACTCCGGTG 
TACAATCGAT TCACACCAAA GCCGTACACA AGTTCTGCCC GGCCATTTGA ACGCAAATTT 
GAAAGTCCGA AGTTCAACCA TAATCTTCTG CCAAGTGAAA CTGTACATAA ACCTGAATTG 
TCTTCAAAAA CTCCCACTTC CCCAAAAACT CTTATGAAAG CTCATAGTTC AACACAGCCT 
CCAGAGTTTG ACAGTGGAGT TGAAACTTTC TCTGTTCACA CAGATAAGCC TAAATATCAA 
ATGAATAATA TCAGCACCAT GCCTAAAGCT GTCCCTGTGA GTCCTTCAGC TGTGGAAGAA 
GATGAAGATG AGGATGGTCA TACTGTAGTG GCTACAGCTC GTGGCATTTT TAACAGCAAT 
GGTGGTGTGT TGAGTTCCAT AGAAACCGGT GTTAGTATAA TTATCCCACA AGGAGCCATT 
CCTGAAGGAA TTGAGCAAGA AATCTATTTC AAAGTCTGCA GAGACAATAG CATTCTCCCA 
CCTTTAGATA AAGAGAAAGG TGAAACTCTG CTGAGCCCCC TAGTGATGTG TGGGCCCCAT 
GGCCTCAAGT TCCTGAAGCC CGTGGAGCTA CGCTTGCCAC ACTGTGCGTC CATGACTCCT 
GACGGTTGGT CTTTTGCTCT AAAATCATCC GACTCCTCGT CGGGTGACCC TAAAACCTGG 
CAAAACAAGT GTCTTCCTGG AGATCCGAAT TACCTTGTTG GAGCCAACTG TGTTTCTGTC 
CTGATTGACC ACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tjp1 ( XM_006540784.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006540847.1 Mus musculus tight junction protein 1 (Tjp1), transcript variant X8, mRNA.

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CloneID	OMu38774
Clone ID Related Accession (Same CDS sequence)	XM_006540784.4 , XM_006540784.3
Accession Version	XM_006540784.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5349bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	tight junction protein ZO-1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006540784.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGCCA GGGCCGCGGC CGCTAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAGCACACAG TGACGCTTCA CAGGGCTCCT GGGTTTGGAT TTGGAATTGC AATCTCTGGT 
GGAAGAGATA ATCCTCATTT TCAGAGTGGG GAAACCTCCA TAGTGATTTC TGATGTGTTA 
AAAGGAGGGC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA 
GTTTCAATGG ATAACGTTGA ACATGCTTTT GCTGTTCAGC AGCTAAGGAA GAGTGGGAAA 
AACGCAAAAA TTACTATCCG AAGGAAGAAG AAAGTTCAGA TCCCTGTAAG TCACCCAGAT 
CCTGAGCCGG TGTCTGATAA TGAAGACGAT AGTTATGACG AAGAAGTGCA TGACCCAAGA 
GCTGGCCGCG GTGCTTTAGC GAACAGAAGG AGCGAGAAGA GCTGGGCAAG GGATAGGAGT 
GCAAGCAGGG AGAGGAGCCT GTCCCCTCGC TCGGACAGGC GGTCCGTGGC CTCCAGTCAG 
CCCGCAAAGC CCACCAAGGT CACACTGGTG AAGTCTCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCCA GCCATATATT TGTAAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGTAACATTC AAGAAGGGGA TGTTGTCTTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAAAC ACTGATAGAA AGGTCTAAAG GCAAGTTAAA AATGGTAGTG 
CAAAGAGATG AGCGGGCTAC CTTACTGAAC GTCCCTGACC TTTCGGATAG TATCCATTCT 
GCTAATGCCT CGGAAAGAGA TGACATTTCA GAAATTCAGT CACTAGCATC AGACCATTCA 
GGTCGCTCGC ATGACAGGCC ACCCCGCCGC AGCCAGTCAC GATCTCCTGA CCAACGTTCA 
GAGCCCTCCG ATCATTCCAC GCAGTCTCCA CAGCAGCCCA GCAATGGCAG TCTCCGGAGC 
AGAGAGGAAG AGCGAATGTC TAAACCTGGG GCCATCTCAA CTCCTGTAAA ACATGTAGAC 
GATCATCCAC CCAAAGCAGT GGAAGAAGTT ACAGTTGAGA AAAATGAGAA GCAGACGCCC 
ACTCTTCCAG AACCGAAACC TGTGTATGCT CAAGTTGGAC AACCAGATGT GGATTTACCC 
GTCAGCCCTT CTGATGGTGC TCTGCCTAAT TCAGCTCATG AAGACGGGAT ACTTAGGCCC 
AGCATGAAAC TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGACT AGCTGGTGGA 
AATGATGTCG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAAGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTG AACAATGTAG ATTTCACAAA TATCATAAGG 
GAAGAGGCCG TCCTTTTCCT CCTTGACCTC CCTAAAGGTG AAGAAGTGAC CATACTGGCT 
CAGAAGAAGA AGGACGTTTA TCGCCGCATT GTAGAATCAG ATGTAGGAGA TTCATTCTAT 
ATTAGAACGC ATTTTGAATA TGAAAAAGAA TCTCCTTACG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GGGTCGTGGA TACTTTATAC AATGGAAAGC TGGGCTCTTG GCTTGCCATT 
CGAATTGGCA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAACAGTTAG CCAGTGTACA GTACACACTT CCAAAGACAG CGGGTGGTGA TCGGGCAGAC 
TTCTGGAGGT TTCGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAC 
TTGTCAGCTC AGCCAGTTCA AACTAAGTTC CCAGCTTATG AAAGGGTTGT TCTTCGAGAA 
GCTGGATTCC TAAGACCTGT AACCATCTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
TTGGCAAGAG AGGAGCCAGA TATCTATCAG ATTGCAAAAA GTGAACCACG AGATGCTGGG 
ACTGACCATC GTAGCTCTGG CATCATTCGC CTTCATACAA TAAAGCAAAT CATAGATCAG 
GATAAACATG CTTTATTAGA TGTAACGCCA AATGCGGTTG ATCGTCTTAA TTATGCGCAG 
TGGTATCCAA TTGTTGTGTT CCTTAACCCT GACTCTAAGC AAGGTGTAAA AACAATGAGG 
ATGAGGTTGT GTCCGGAGTC TCGGAAAAGC GCCAGGAAGC TATATGAACG CTCTCATAAG 
CTTCGTAAGA ACAATCACCA TCTCTTCACA ACTACAATTA ACTTAAACTC AATGAATGAT 
GGTTGGTACG GTGCCCTGAA AGAAGCGATT CAGCAGCAAC AGAACCAGCT GGTGTGGGTC 
TCTGAGGGGA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA CGATCGTCTG 
TCCTACCTGT CAGCCCCAGG TAGTGAGTAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA TACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTGGG GACTCCCCCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTAAGAGAGG ATTCCTCTGG AATGCATCAT GAAAACCAGA CATACCCTCC TTACTCACCA 
CAAGCGCAGC CACAAGCTAT TCATAGAATA GACTCCCCTG GACTTAAGCC AGCCTCTCAA 
CAGAAAGCAG AAGCCTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAC CCCAGCATCA 
TCAGCCTCTG CAGTTAATCA TAATGTCAGT GTAACTAATG TCAGCCTGGA GGAGCCTGCC 
CCAGCCCCTC CCACCTCGCA CGCATCACAG CCTGGTTGTT TAGGAGCACC AAGTGCTGAG 
GCAGCTCACG TAGTGCTCAG AGGTGAAGGA CCACCATTGC CGCCGCATGC AGACCCAGCA 
AAGGTGTACA GGAAGGAGCC GTATTCTGAA GAAATGATGA GACAAAACCA TATTTTAAAA 
CAACCAGCTC TTGGTCACCC AGGGCAGAGG CCAGATAAAG AGCCAAATCT AGCCTATGAA 
CCCCAACTTC CATATATAGA AAAACAAGCC AGCAGAGACC TTGAGCAGCC GTCATACAGG 
TATGAGGTCT CAAGCTACAC AGACCAGTTT TCTCGGAACT ATGACCATCG CCTACGGTTT 
GAAGATCGAA TCCCTACCTA TGAAGACCAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CAACCTCGGC CTTTTGAGAA TCAGCATCCC CGAGACCTGG ACTCCAGACA ACATCCCGAA 
GAGGCTTCAG AACGAGGTTA TTTCCAGCGT TTTGAAGAGC CAGCCCCTCT GTCGTACGAC 
AGTAGAACAC GCTATGAGCA GCTGCCTCGA ACCTCTACTC TACGACATGA AGAGCAGCCA 
GCCCCTGCAT ATGAGGTGCA CAACAGGTAC AGGCCAGAGG CACAGCCCTA TTCTTCAACA 
GGCCCTAAGT CATCTGAGCC CAAGCAGTAC TTTGACCAGT ACCCGCGAAG TTATGAGCAA 
GTTCCACCAC CAGGCTTTAC CTCCAAAACA GGCCATTACG AGCCTCTCCA TGGTGCTGCA 
GTTGTCCCTC CTCTGATACC TTCCTCTCAA CAAAAGCCAG AAGTCCTGCC CTCGGCTACC 
AAACCACAGC CTCCACCCCC AACCCTAACT GAGGAGGAGG AGGATCCAGC AATGAAACCA 
CAGTCTGTGC TCACCAGAGT CAAAATGTTT GAAAACAAAA GATCTGCGTC TTTGGAGAAC 
AAGAAAGATG TGAATGACAC TGCCAGCTTT AAGCCTCCGG AAGTAGCATC TAAACCTCCA 
GGTGCTTCTC TTGCTGGCCC TAAACCTGTC CCTCAGAGTC AGTTTAGTGA GCACGACAAA 
ACGCTCTACA GGCTCCCAGA GCCTCAGAAA CCTCAAGTGA AGCCACCCGA AGATATTGTT 
CGATCAAATC ATTACGACCC TGAAGAGGAT GAAGAATATT ACCGGAAACA GCTCTCCTAC 
TTTGACCGAA GAAGTTTTGA GAGCAAGCCT TCTGCACATC TTCCTGCTGG CCATCACTCA 
GAGCCTGCTA AGCCAGTCCA TTCTCAGAGC CAGCCCAACT TCTCTAGTTA CTCTTCAAAA 
TTTCTTGGCT CTTACACTAG CTATGACTAC TTGAAAAGGA ACATCAAGTG GGGAAAACCC 
GAAACTGATG CTGTGGATAG ATCATTCAGT GAGAAACGTT ATGATCCAGC CCAGGCCACG 
CCTCCTCCTC CTCCGTTGCC CTCACAGTAC AGCCAGCCAG CTCCACCTCT GTCCAGCTCT 
TCTCTCCACA TACATTCCAA GGGCGCCCAG GGTGAAGGCA ACTCAGTATC ATTGGATTTT 
CAGAACTCAT ATATGTCCAA ACCAGACCCA CCCCCATCTC AGAGTAAACC AGCAACTTTC 
AGACCACCAA CTCGAGAAGA CCCCCCTCAG ACTTTCTATC CGCAGAAAAG TTTCCCAGAC 
AAAGCTCCAG TTAACGGAGC TGAGCAGACT CAGAAAACCA TCACTCCGGT GTACAATCGA 
TTCACACCAA AGCCGTACAC AAGTTCTGCC CGGCCATTTG AACGCAAATT TGAAAGTCCG 
AAGTTCAACC ATAATCTTCT GCCAAGTGAA ACTGTACATA AACCTGAATT GTCTTCAAAA 
ACTCCCACTT CCCCAAAAAC TCTTATGAAA GCTCATAGTT CAACACAGCC TCCAGAGTTT 
GACAGTGGAG TTGAAACTTT CTCTGTTCAC ACAGATAAGC CTAAATATCA AATGAATAAT 
ATCAGCACCA TGCCTAAAGC TGTCCCTGTG AGTCCTTCAG CTGTGGAAGA AGATGAAGAT 
GAGGATGGTC ATACTGTAGT GGCTACAGCT CGTGGCATTT TTAACAGCAA TGGTGGTGTG 
TTGAGTTCCA TAGAAACCGG TGTTAGTATA ATTATCCCAC AAGGAGCCAT TCCTGAAGGA 
ATTGAGCAAG AAATCTATTT CAAAGTCTGC AGAGACAATA GCATTCTCCC ACCTTTAGAT 
AAAGAGAAAG GTGAAACTCT GCTGAGCCCC CTAGTGATGT GTGGGCCCCA TGGCCTCAAG 
TTCCTGAAGC CCGTGGAGCT ACGCTTGCCA CACTGTGCGT CCATGACTCC TGACGGTTGG 
TCTTTTGCTC TAAAATCATC CGACTCCTCG TCGGGTGACC CTAAAACCTG GCAAAACAAG 
TGTCTTCCTG GAGATCCGAA TTACCTTGTT GGAGCCAACT GTGTTTCTGT CCTGATTGAC 
CACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006540847.1
CDS	846..6194
Translation	MSARAAAAKSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSHPDPEPVSDNEDDSYDEEVHDPRAGRGALANRRSEKSWARDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSQSRSPDQRSEPSDHSTQSPQQPSNGSLRSREEERMSKPGAISTPVKHVDDHPPKAVEEVTVEKNEKQTPTLPEPKPVYAQVGQPDVDLPVSPSDGALPNSAHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDHRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQAIHRIDSPGLKPASQQKAEASSPVPYLSPETTPASSASAVNHNVSVTNVSLEEPAPAPPTSHASQPGCLGAPSAEAAHVVLRGEGPPLPPHADPAKVYRKEPYSEEMMRQNHILKQPALGHPGQRPDKEPNLAYEPQLPYIEKQASRDLEQPSYRYEVSSYTDQFSRNYDHRLRFEDRIPTYEDQWSYYDDKQPYQPRPFENQHPRDLDSRQHPEEASERGYFQRFEEPAPLSYDSRTRYEQLPRTSTLRHEEQPAPAYEVHNRYRPEAQPYSSTGPKSSEPKQYFDQYPRSYEQVPPPGFTSKTGHYEPLHGAAVVPPLIPSSQQKPEVLPSATKPQPPPPTLTEEEEDPAMKPQSVLTRVKMFENKRSASLENKKDVNDTASFKPPEVASKPPGASLAGPKPVPQSQFSEHDKTLYRLPEPQKPQVKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFESKPSAHLPAGHHSEPAKPVHSQSQPNFSSYSSKFLGSYTSYDYLKRNIKWGKPETDAVDRSFSEKRYDPAQATPPPPPLPSQYSQPAPPLSSSSLHIHSKGAQGEGNSVSLDFQNSYMSKPDPPPSQSKPATFRPPTREDPPQTFYPQKSFPDKAPVNGAEQTQKTITPVYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETVHKPELSSKTPTSPKTLMKAHSSTQPPEFDSGVETFSVHTDKPKYQMNNISTMPKAVPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_006540784.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus tight junction protein 1 (Tjp1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006540784.3

ORF Insert Sequence:

ATGTCCGCCA GGGCCGCGGC CGCTAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAGCACACAG TGACGCTTCA CAGGGCTCCT GGGTTTGGAT TTGGAATTGC AATCTCTGGT 
GGAAGAGATA ATCCTCATTT TCAGAGTGGG GAAACCTCCA TAGTGATTTC TGATGTGTTA 
AAAGGAGGGC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA 
GTTTCAATGG ATAACGTTGA ACATGCTTTT GCTGTTCAGC AGCTAAGGAA GAGTGGGAAA 
AACGCAAAAA TTACTATCCG AAGGAAGAAG AAAGTTCAGA TCCCTGTAAG TCACCCAGAT 
CCTGAGCCGG TGTCTGATAA TGAAGACGAT AGTTATGACG AAGAAGTGCA TGACCCAAGA 
GCTGGCCGCG GTGCTTTAGC GAACAGAAGG AGCGAGAAGA GCTGGGCAAG GGATAGGAGT 
GCAAGCAGGG AGAGGAGCCT GTCCCCTCGC TCGGACAGGC GGTCCGTGGC CTCCAGTCAG 
CCCGCAAAGC CCACCAAGGT CACACTGGTG AAGTCTCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCCA GCCATATATT TGTAAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGTAACATTC AAGAAGGGGA TGTTGTCTTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAAAC ACTGATAGAA AGGTCTAAAG GCAAGTTAAA AATGGTAGTG 
CAAAGAGATG AGCGGGCTAC CTTACTGAAC GTCCCTGACC TTTCGGATAG TATCCATTCT 
GCTAATGCCT CGGAAAGAGA TGACATTTCA GAAATTCAGT CACTAGCATC AGACCATTCA 
GGTCGCTCGC ATGACAGGCC ACCCCGCCGC AGCCAGTCAC GATCTCCTGA CCAACGTTCA 
GAGCCCTCCG ATCATTCCAC GCAGTCTCCA CAGCAGCCCA GCAATGGCAG TCTCCGGAGC 
AGAGAGGAAG AGCGAATGTC TAAACCTGGG GCCATCTCAA CTCCTGTAAA ACATGTAGAC 
GATCATCCAC CCAAAGCAGT GGAAGAAGTT ACAGTTGAGA AAAATGAGAA GCAGACGCCC 
ACTCTTCCAG AACCGAAACC TGTGTATGCT CAAGTTGGAC AACCAGATGT GGATTTACCC 
GTCAGCCCTT CTGATGGTGC TCTGCCTAAT TCAGCTCATG AAGACGGGAT ACTTAGGCCC 
AGCATGAAAC TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGACT AGCTGGTGGA 
AATGATGTCG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAAGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTG AACAATGTAG ATTTCACAAA TATCATAAGG 
GAAGAGGCCG TCCTTTTCCT CCTTGACCTC CCTAAAGGTG AAGAAGTGAC CATACTGGCT 
CAGAAGAAGA AGGACGTTTA TCGCCGCATT GTAGAATCAG ATGTAGGAGA TTCATTCTAT 
ATTAGAACGC ATTTTGAATA TGAAAAAGAA TCTCCTTACG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GGGTCGTGGA TACTTTATAC AATGGAAAGC TGGGCTCTTG GCTTGCCATT 
CGAATTGGCA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAACAGTTAG CCAGTGTACA GTACACACTT CCAAAGACAG CGGGTGGTGA TCGGGCAGAC 
TTCTGGAGGT TTCGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAC 
TTGTCAGCTC AGCCAGTTCA AACTAAGTTC CCAGCTTATG AAAGGGTTGT TCTTCGAGAA 
GCTGGATTCC TAAGACCTGT AACCATCTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
TTGGCAAGAG AGGAGCCAGA TATCTATCAG ATTGCAAAAA GTGAACCACG AGATGCTGGG 
ACTGACCATC GTAGCTCTGG CATCATTCGC CTTCATACAA TAAAGCAAAT CATAGATCAG 
GATAAACATG CTTTATTAGA TGTAACGCCA AATGCGGTTG ATCGTCTTAA TTATGCGCAG 
TGGTATCCAA TTGTTGTGTT CCTTAACCCT GACTCTAAGC AAGGTGTAAA AACAATGAGG 
ATGAGGTTGT GTCCGGAGTC TCGGAAAAGC GCCAGGAAGC TATATGAACG CTCTCATAAG 
CTTCGTAAGA ACAATCACCA TCTCTTCACA ACTACAATTA ACTTAAACTC AATGAATGAT 
GGTTGGTACG GTGCCCTGAA AGAAGCGATT CAGCAGCAAC AGAACCAGCT GGTGTGGGTC 
TCTGAGGGGA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA CGATCGTCTG 
TCCTACCTGT CAGCCCCAGG TAGTGAGTAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA TACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTGGG GACTCCCCCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTAAGAGAGG ATTCCTCTGG AATGCATCAT GAAAACCAGA CATACCCTCC TTACTCACCA 
CAAGCGCAGC CACAAGCTAT TCATAGAATA GACTCCCCTG GACTTAAGCC AGCCTCTCAA 
CAGAAAGCAG AAGCCTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAC CCCAGCATCA 
TCAGCCTCTG CAGTTAATCA TAATGTCAGT GTAACTAATG TCAGCCTGGA GGAGCCTGCC 
CCAGCCCCTC CCACCTCGCA CGCATCACAG CCTGGTTGTT TAGGAGCACC AAGTGCTGAG 
GCAGCTCACG TAGTGCTCAG AGGTGAAGGA CCACCATTGC CGCCGCATGC AGACCCAGCA 
AAGGTGTACA GGAAGGAGCC GTATTCTGAA GAAATGATGA GACAAAACCA TATTTTAAAA 
CAACCAGCTC TTGGTCACCC AGGGCAGAGG CCAGATAAAG AGCCAAATCT AGCCTATGAA 
CCCCAACTTC CATATATAGA AAAACAAGCC AGCAGAGACC TTGAGCAGCC GTCATACAGG 
TATGAGGTCT CAAGCTACAC AGACCAGTTT TCTCGGAACT ATGACCATCG CCTACGGTTT 
GAAGATCGAA TCCCTACCTA TGAAGACCAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CAACCTCGGC CTTTTGAGAA TCAGCATCCC CGAGACCTGG ACTCCAGACA ACATCCCGAA 
GAGGCTTCAG AACGAGGTTA TTTCCAGCGT TTTGAAGAGC CAGCCCCTCT GTCGTACGAC 
AGTAGAACAC GCTATGAGCA GCTGCCTCGA ACCTCTACTC TACGACATGA AGAGCAGCCA 
GCCCCTGCAT ATGAGGTGCA CAACAGGTAC AGGCCAGAGG CACAGCCCTA TTCTTCAACA 
GGCCCTAAGT CATCTGAGCC CAAGCAGTAC TTTGACCAGT ACCCGCGAAG TTATGAGCAA 
GTTCCACCAC CAGGCTTTAC CTCCAAAACA GGCCATTACG AGCCTCTCCA TGGTGCTGCA 
GTTGTCCCTC CTCTGATACC TTCCTCTCAA CAAAAGCCAG AAGTCCTGCC CTCGGCTACC 
AAACCACAGC CTCCACCCCC AACCCTAACT GAGGAGGAGG AGGATCCAGC AATGAAACCA 
CAGTCTGTGC TCACCAGAGT CAAAATGTTT GAAAACAAAA GATCTGCGTC TTTGGAGAAC 
AAGAAAGATG TGAATGACAC TGCCAGCTTT AAGCCTCCGG AAGTAGCATC TAAACCTCCA 
GGTGCTTCTC TTGCTGGCCC TAAACCTGTC CCTCAGAGTC AGTTTAGTGA GCACGACAAA 
ACGCTCTACA GGCTCCCAGA GCCTCAGAAA CCTCAAGTGA AGCCACCCGA AGATATTGTT 
CGATCAAATC ATTACGACCC TGAAGAGGAT GAAGAATATT ACCGGAAACA GCTCTCCTAC 
TTTGACCGAA GAAGTTTTGA GAGCAAGCCT TCTGCACATC TTCCTGCTGG CCATCACTCA 
GAGCCTGCTA AGCCAGTCCA TTCTCAGAGC CAGCCCAACT TCTCTAGTTA CTCTTCAAAA 
TTTCTTGGCT CTTACACTAG CTATGACTAC TTGAAAAGGA ACATCAAGTG GGGAAAACCC 
GAAACTGATG CTGTGGATAG ATCATTCAGT GAGAAACGTT ATGATCCAGC CCAGGCCACG 
CCTCCTCCTC CTCCGTTGCC CTCACAGTAC AGCCAGCCAG CTCCACCTCT GTCCAGCTCT 
TCTCTCCACA TACATTCCAA GGGCGCCCAG GGTGAAGGCA ACTCAGTATC ATTGGATTTT 
CAGAACTCAT ATATGTCCAA ACCAGACCCA CCCCCATCTC AGAGTAAACC AGCAACTTTC 
AGACCACCAA CTCGAGAAGA CCCCCCTCAG ACTTTCTATC CGCAGAAAAG TTTCCCAGAC 
AAAGCTCCAG TTAACGGAGC TGAGCAGACT CAGAAAACCA TCACTCCGGT GTACAATCGA 
TTCACACCAA AGCCGTACAC AAGTTCTGCC CGGCCATTTG AACGCAAATT TGAAAGTCCG 
AAGTTCAACC ATAATCTTCT GCCAAGTGAA ACTGTACATA AACCTGAATT GTCTTCAAAA 
ACTCCCACTT CCCCAAAAAC TCTTATGAAA GCTCATAGTT CAACACAGCC TCCAGAGTTT 
GACAGTGGAG TTGAAACTTT CTCTGTTCAC ACAGATAAGC CTAAATATCA AATGAATAAT 
ATCAGCACCA TGCCTAAAGC TGTCCCTGTG AGTCCTTCAG CTGTGGAAGA AGATGAAGAT 
GAGGATGGTC ATACTGTAGT GGCTACAGCT CGTGGCATTT TTAACAGCAA TGGTGGTGTG 
TTGAGTTCCA TAGAAACCGG TGTTAGTATA ATTATCCCAC AAGGAGCCAT TCCTGAAGGA 
ATTGAGCAAG AAATCTATTT CAAAGTCTGC AGAGACAATA GCATTCTCCC ACCTTTAGAT 
AAAGAGAAAG GTGAAACTCT GCTGAGCCCC CTAGTGATGT GTGGGCCCCA TGGCCTCAAG 
TTCCTGAAGC CCGTGGAGCT ACGCTTGCCA CACTGTGCGT CCATGACTCC TGACGGTTGG 
TCTTTTGCTC TAAAATCATC CGACTCCTCG TCGGGTGACC CTAAAACCTG GCAAAACAAG 
TGTCTTCCTG GAGATCCGAA TTACCTTGTT GGAGCCAACT GTGTTTCTGT CCTGATTGAC 
CACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tjp1 ( XM_006540778.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006540841.1 Mus musculus tight junction protein 1 (Tjp1), transcript variant X1, mRNA.

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CloneID	OMu38768
Clone ID Related Accession (Same CDS sequence)	XM_006540778.3 , XM_006540778.4
Accession Version	XM_006540778.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5628bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	tight junction protein ZO-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006540778.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT 
CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA 
CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC 
AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA 
CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA 
AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT 
GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG 
AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG 
TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC 
ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT 
GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT 
GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA 
CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC 
TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT 
GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA 
CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG 
CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT 
AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG 
GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT 
GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT 
CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC 
CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT 
CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT 
GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT 
ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG 
TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA 
ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA 
ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT 
ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC 
ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT 
GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC 
CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT 
CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT 
CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA 
GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT 
AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG 
ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA 
ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT 
CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCCTCATCT 
CCAGTCCCTT ACCTTTCGCC TGAAACAACC CCAGCATCAT CAGCCTCTGC AGTTAATCAT 
AATGTCAGTG TAACTAATGT CAGCCTGGAG GAGCCTGCCC CAGCCCCTCC CACCTCGCAC 
GCATCACAGC CTGGTTGTTT AGGAGCACCA AGTGCTGAGG CAGCTCACGT AGTGCTCAGA 
GGTGAAGGAC CACCATTGCC GCCGCATGCA GACCCAGCAA AGGTGTACAG GAAGGAGCCG 
TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA 
GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA 
AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA 
GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT 
GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT 
CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT 
TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG 
CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC 
AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC 
AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC 
TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT 
TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA 
ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC 
AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT 
GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT 
AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG 
CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT 
GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG 
AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT 
TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAAT TTCTTGGCTC TTACACTAGC 
TATGACTACT TGAAAAGGAA CATCAAGTGG GGAAAACCCG AAACTGATGC TGTGGATAGA 
TCATTCAGTG AGAAACGTTA TGATCCAGCC CAGGCCACGC CTCCTCCTCC TCCGTTGCCC 
TCACAGTACA GCCAGCCAGC TCCACCTCTG TCCAGCTCTT CTCTCCACAT ACATTCCAAG 
GGCGCCCAGG GTGAAGGCAA CTCAGTATCA TTGGATTTTC AGAACTCATA TATGTCCAAA 
CCAGACCCAC CCCCATCTCA GAGTAAACCA GCAACTTTCA GACCACCAAC TCGAGAAGAC 
CCCCCTCAGA CTTTCTATCC GCAGAAAAGT TTCCCAGACA AAGCTCCAGT TAACGGAGCT 
GAGCAGACTC AGAAAACCAT CACTCCGGTG TACAATCGAT TCACACCAAA GCCGTACACA 
AGTTCTGCCC GGCCATTTGA ACGCAAATTT GAAAGTCCGA AGTTCAACCA TAATCTTCTG 
CCAAGTGAAA CTGTACATAA ACCTGAATTG TCTTCAAAAA CTCCCACTTC CCCAAAAACT 
CTTATGAAAG CTCATAGTTC AACACAGCCT CCAGAGTTTG ACAGTGGAGT TGAAACTTTC 
TCTGTTCACA CAGATAAGCC TAAATATCAA ATGAATAATA TCAGCACCAT GCCTAAAGCT 
GTCCCTGTGA GTCCTTCAGC TGTGGAAGAA GATGAAGATG AGGATGGTCA TACTGTAGTG 
GCTACAGCTC GTGGCATTTT TAACAGCAAT GGTGGTGTGT TGAGTTCCAT AGAAACCGGT 
GTTAGTATAA TTATCCCACA AGGAGCCATT CCTGAAGGAA TTGAGCAAGA AATCTATTTC 
AAAGTCTGCA GAGACAATAG CATTCTCCCA CCTTTAGATA AAGAGAAAGG TGAAACTCTG 
CTGAGCCCCC TAGTGATGTG TGGGCCCCAT GGCCTCAAGT TCCTGAAGCC CGTGGAGCTA 
CGCTTGCCAC ACTGTGCGTC CATGACTCCT GACGGTTGGT CTTTTGCTCT AAAATCATCC 
GACTCCTCGT CGGGTGACCC TAAAACCTGG CAAAACAAGT GTCTTCCTGG AGATCCGAAT 
TACCTTGTTG GAGCCAACTG TGTTTCTGTC CTGATTGACC ACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006540841.1
CDS	73..5700
Translation	MRRLEGLCLRRRRRRRGPARERSGDKMKYQKYLTVLQMAIGVTPSNRGSLLPLKRKLWVTPSSENPHGATSTVSQGKPSLRRIKGRLHRSKSLDSIDFCELTSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSHPDPEPVSDNEDDSYDEEVHDPRAGRGALANRRSEKSWARDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSQSRSPDQRSEPSDHSTQSPQQPSNGSLRSREEERMSKPGAISTPVKHVDDHPPKAVEEVTVEKNEKQTPTLPEPKPVYAQVGQPDVDLPVSPSDGALPNSAHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDHRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQAIHRIDSPGLKPASQQKAEASSPVPYLSPETTPASSASAVNHNVSVTNVSLEEPAPAPPTSHASQPGCLGAPSAEAAHVVLRGEGPPLPPHADPAKVYRKEPYSEEMMRQNHILKQPALGHPGQRPDKEPNLAYEPQLPYIEKQASRDLEQPSYRYEVSSYTDQFSRNYDHRLRFEDRIPTYEDQWSYYDDKQPYQPRPFENQHPRDLDSRQHPEEASERGYFQRFEEPAPLSYDSRTRYEQLPRTSTLRHEEQPAPAYEVHNRYRPEAQPYSSTGPKSSEPKQYFDQYPRSYEQVPPPGFTSKTGHYEPLHGAAVVPPLIPSSQQKPEVLPSATKPQPPPPTLTEEEEDPAMKPQSVLTRVKMFENKRSASLENKKDVNDTASFKPPEVASKPPGASLAGPKPVPQSQFSEHDKTLYRLPEPQKPQVKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFESKPSAHLPAGHHSEPAKPVHSQSQPNFSSYSSKFLGSYTSYDYLKRNIKWGKPETDAVDRSFSEKRYDPAQATPPPPPLPSQYSQPAPPLSSSSLHIHSKGAQGEGNSVSLDFQNSYMSKPDPPPSQSKPATFRPPTREDPPQTFYPQKSFPDKAPVNGAEQTQKTITPVYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETVHKPELSSKTPTSPKTLMKAHSSTQPPEFDSGVETFSVHTDKPKYQMNNISTMPKAVPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_006540778.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus tight junction protein 1 (Tjp1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006540778.3

ORF Insert Sequence:

ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT 
CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA 
CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC 
AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA 
CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA 
AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT 
GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG 
AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG 
TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC 
ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT 
GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT 
GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA 
CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC 
TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT 
GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA 
CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG 
CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT 
AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG 
GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT 
GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT 
CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC 
CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT 
CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT 
GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT 
ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG 
TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA 
ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA 
ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT 
ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC 
ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT 
GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC 
CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT 
CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT 
CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA 
GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT 
AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG 
ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA 
ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT 
CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCCTCATCT 
CCAGTCCCTT ACCTTTCGCC TGAAACAACC CCAGCATCAT CAGCCTCTGC AGTTAATCAT 
AATGTCAGTG TAACTAATGT CAGCCTGGAG GAGCCTGCCC CAGCCCCTCC CACCTCGCAC 
GCATCACAGC CTGGTTGTTT AGGAGCACCA AGTGCTGAGG CAGCTCACGT AGTGCTCAGA 
GGTGAAGGAC CACCATTGCC GCCGCATGCA GACCCAGCAA AGGTGTACAG GAAGGAGCCG 
TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA 
GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA 
AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA 
GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT 
GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT 
CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT 
TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG 
CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC 
AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC 
AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC 
TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT 
TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA 
ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC 
AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT 
GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT 
AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG 
CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT 
GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG 
AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT 
TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAAT TTCTTGGCTC TTACACTAGC 
TATGACTACT TGAAAAGGAA CATCAAGTGG GGAAAACCCG AAACTGATGC TGTGGATAGA 
TCATTCAGTG AGAAACGTTA TGATCCAGCC CAGGCCACGC CTCCTCCTCC TCCGTTGCCC 
TCACAGTACA GCCAGCCAGC TCCACCTCTG TCCAGCTCTT CTCTCCACAT ACATTCCAAG 
GGCGCCCAGG GTGAAGGCAA CTCAGTATCA TTGGATTTTC AGAACTCATA TATGTCCAAA 
CCAGACCCAC CCCCATCTCA GAGTAAACCA GCAACTTTCA GACCACCAAC TCGAGAAGAC 
CCCCCTCAGA CTTTCTATCC GCAGAAAAGT TTCCCAGACA AAGCTCCAGT TAACGGAGCT 
GAGCAGACTC AGAAAACCAT CACTCCGGTG TACAATCGAT TCACACCAAA GCCGTACACA 
AGTTCTGCCC GGCCATTTGA ACGCAAATTT GAAAGTCCGA AGTTCAACCA TAATCTTCTG 
CCAAGTGAAA CTGTACATAA ACCTGAATTG TCTTCAAAAA CTCCCACTTC CCCAAAAACT 
CTTATGAAAG CTCATAGTTC AACACAGCCT CCAGAGTTTG ACAGTGGAGT TGAAACTTTC 
TCTGTTCACA CAGATAAGCC TAAATATCAA ATGAATAATA TCAGCACCAT GCCTAAAGCT 
GTCCCTGTGA GTCCTTCAGC TGTGGAAGAA GATGAAGATG AGGATGGTCA TACTGTAGTG 
GCTACAGCTC GTGGCATTTT TAACAGCAAT GGTGGTGTGT TGAGTTCCAT AGAAACCGGT 
GTTAGTATAA TTATCCCACA AGGAGCCATT CCTGAAGGAA TTGAGCAAGA AATCTATTTC 
AAAGTCTGCA GAGACAATAG CATTCTCCCA CCTTTAGATA AAGAGAAAGG TGAAACTCTG 
CTGAGCCCCC TAGTGATGTG TGGGCCCCAT GGCCTCAAGT TCCTGAAGCC CGTGGAGCTA 
CGCTTGCCAC ACTGTGCGTC CATGACTCCT GACGGTTGGT CTTTTGCTCT AAAATCATCC 
GACTCCTCGT CGGGTGACCC TAAAACCTGG CAAAACAAGT GTCTTCCTGG AGATCCGAAT 
TACCTTGTTG GAGCCAACTG TGTTTCTGTC CTGATTGACC ACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tjp1 ( XM_006540779.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006540842.1 Mus musculus tight junction protein 1 (Tjp1), transcript variant X2, mRNA.

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CloneID	OMu38769
Clone ID Related Accession (Same CDS sequence)	XM_006540779.3 , XM_006540779.4
Accession Version	XM_006540779.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	tight junction protein ZO-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006540779.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT 
CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA 
CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC 
AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA 
CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA 
AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT 
GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG 
AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG 
TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC 
ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT 
GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT 
GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA 
CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC 
TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT 
GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA 
CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG 
CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT 
AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG 
GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT 
GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT 
CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC 
CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT 
CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT 
GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT 
ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG 
TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA 
ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA 
ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT 
ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC 
ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT 
GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC 
CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT 
CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT 
CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA 
GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT 
AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG 
ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA 
ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT 
CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCCTCATCT 
CCAGTCCCTT ACCTTTCGCC TGAAACAACC CCAGCATCAT CAGCCTCTGC AGTTAATCAT 
AATGTCAGTG TAACTAATGT CAGCCTGGAG GAGCCTGCCC CAGCCCCTCC CACCTCGCAC 
GCATCACAGC CTGGTTGTTT AGGAGCACCA AGTGCTGAGG CAGCTCACGT AGTGCTCAGA 
GGTGAAGGAC CACCATTGCC GCCGCATGCA GACCCAGCAA AGGTGTACAG GAAGGAGCCG 
TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA 
GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA 
AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA 
GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT 
GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT 
CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT 
TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG 
CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC 
AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC 
AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC 
TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT 
TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA 
ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC 
AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT 
GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT 
AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG 
CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT 
GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG 
AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT 
TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAAT TTCTTGGCTC TTACACTAGC 
TATGACTACT TGAAAAGGAA CATCAAGTGG GGAAAACCCG AAACTGATGC TGTGGATAGA 
TCATTCAGTG AGAAACGTTA TGATCCAGCC CAGGCCACGC CTCCTCCTCC TCCGTTGCCC 
TCACAGTACA GCCAGCCAGC TCCACCTCTG TCCAGCTCTT CTCTCCACAT ACATTCCAAG 
GGCGCCCAGG GTGAAGGCAA CTCAGTATCA TTGGATTTTC AGAACTCATA TATGTCCAAA 
CCAGACCCAC CCCCATCTCA GAGTAAACCA GCAACTTTCA GACCACCAAC TCGAGAAGAC 
CCCCCTCAGA CTTTCTATCC GCAGAAAAGT TTCCCAGACA AAGCTCCAGT TAACGGAGCT 
GAGCAGACTC AGAAAACCAT CACTCCGGTG TACAATCGAT TCACACCAAA GCCGTACACA 
AGTTCTGCCC GGCCATTTGA ACGCAAATTT GAAAGTCCGA AGTTCAACCA TAATCTTCTG 
CCAAGTGAAA CTGTACATAA ACCTGAATTG TCTTCAAAAA CTCCCACTTC CCCAAAAACT 
CTTATGAAAG CTCATAGTTC AACACAGCCT CCAGAGTTTG ACAGTGGAGT TGAAACTTTC 
TCTGTTCACA CAGATAAGCC TAAATATCAA ATGAATAATA TCAGCACCAT GCCTAAAGCT 
GTCCCTGTGA GTCCTTCAGC TGTGGAAGAA GATGAAGATG AGGATGGTCA TACTGTAGTG 
GCTACAGCTC GTGGCATTTT TAACAGCAAT GGTGGTGTGT TGAGTTCCAT AGAAACCGGT 
GTTAGTATAA TTATCCCACA AGGAGCCATT CCTGAAGGAA TTGAGCAAGA AATCTATTTC 
AAAGTCTGCA GAGACAATAG CATTCTCCCA CCTTTAGATA AAGAGAAAGG TGAAACTCTG 
CTGAGCCCCC TAGTGATGTG TGGGCCCCAT GGCCTCAAGT TCCTGAAGCC CGTGGAGCTA 
CGCTTGCCAC ACTGTGCGTC CATGACTCCT GACGGTGACC CTAAAACCTG GCAAAACAAG 
TGTCTTCCTG GAGATCCGAA TTACCTTGTT GGAGCCAACT GTGTTTCTGT CCTGATTGAC 
CACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006540842.1
CDS	73..5661
Translation	MRRLEGLCLRRRRRRRGPARERSGDKMKYQKYLTVLQMAIGVTPSNRGSLLPLKRKLWVTPSSENPHGATSTVSQGKPSLRRIKGRLHRSKSLDSIDFCELTSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSHPDPEPVSDNEDDSYDEEVHDPRAGRGALANRRSEKSWARDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSQSRSPDQRSEPSDHSTQSPQQPSNGSLRSREEERMSKPGAISTPVKHVDDHPPKAVEEVTVEKNEKQTPTLPEPKPVYAQVGQPDVDLPVSPSDGALPNSAHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDHRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQAIHRIDSPGLKPASQQKAEASSPVPYLSPETTPASSASAVNHNVSVTNVSLEEPAPAPPTSHASQPGCLGAPSAEAAHVVLRGEGPPLPPHADPAKVYRKEPYSEEMMRQNHILKQPALGHPGQRPDKEPNLAYEPQLPYIEKQASRDLEQPSYRYEVSSYTDQFSRNYDHRLRFEDRIPTYEDQWSYYDDKQPYQPRPFENQHPRDLDSRQHPEEASERGYFQRFEEPAPLSYDSRTRYEQLPRTSTLRHEEQPAPAYEVHNRYRPEAQPYSSTGPKSSEPKQYFDQYPRSYEQVPPPGFTSKTGHYEPLHGAAVVPPLIPSSQQKPEVLPSATKPQPPPPTLTEEEEDPAMKPQSVLTRVKMFENKRSASLENKKDVNDTASFKPPEVASKPPGASLAGPKPVPQSQFSEHDKTLYRLPEPQKPQVKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFESKPSAHLPAGHHSEPAKPVHSQSQPNFSSYSSKFLGSYTSYDYLKRNIKWGKPETDAVDRSFSEKRYDPAQATPPPPPLPSQYSQPAPPLSSSSLHIHSKGAQGEGNSVSLDFQNSYMSKPDPPPSQSKPATFRPPTREDPPQTFYPQKSFPDKAPVNGAEQTQKTITPVYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETVHKPELSSKTPTSPKTLMKAHSSTQPPEFDSGVETFSVHTDKPKYQMNNISTMPKAVPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_006540779.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus tight junction protein 1 (Tjp1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006540779.3

ORF Insert Sequence:

ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT 
CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA 
CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC 
AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA 
CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA 
AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT 
GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG 
AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG 
TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC 
ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT 
GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT 
GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA 
CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC 
TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT 
GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA 
CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG 
CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT 
AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG 
GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT 
GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT 
CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC 
CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT 
CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT 
GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT 
ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG 
TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA 
ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA 
ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT 
ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC 
ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT 
GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC 
CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT 
CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT 
CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA 
GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT 
AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG 
ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA 
ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT 
CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCCTCATCT 
CCAGTCCCTT ACCTTTCGCC TGAAACAACC CCAGCATCAT CAGCCTCTGC AGTTAATCAT 
AATGTCAGTG TAACTAATGT CAGCCTGGAG GAGCCTGCCC CAGCCCCTCC CACCTCGCAC 
GCATCACAGC CTGGTTGTTT AGGAGCACCA AGTGCTGAGG CAGCTCACGT AGTGCTCAGA 
GGTGAAGGAC CACCATTGCC GCCGCATGCA GACCCAGCAA AGGTGTACAG GAAGGAGCCG 
TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA 
GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA 
AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA 
GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT 
GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT 
CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT 
TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG 
CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC 
AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC 
AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC 
TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT 
TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA 
ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC 
AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT 
GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT 
AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG 
CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT 
GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG 
AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT 
TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAAT TTCTTGGCTC TTACACTAGC 
TATGACTACT TGAAAAGGAA CATCAAGTGG GGAAAACCCG AAACTGATGC TGTGGATAGA 
TCATTCAGTG AGAAACGTTA TGATCCAGCC CAGGCCACGC CTCCTCCTCC TCCGTTGCCC 
TCACAGTACA GCCAGCCAGC TCCACCTCTG TCCAGCTCTT CTCTCCACAT ACATTCCAAG 
GGCGCCCAGG GTGAAGGCAA CTCAGTATCA TTGGATTTTC AGAACTCATA TATGTCCAAA 
CCAGACCCAC CCCCATCTCA GAGTAAACCA GCAACTTTCA GACCACCAAC TCGAGAAGAC 
CCCCCTCAGA CTTTCTATCC GCAGAAAAGT TTCCCAGACA AAGCTCCAGT TAACGGAGCT 
GAGCAGACTC AGAAAACCAT CACTCCGGTG TACAATCGAT TCACACCAAA GCCGTACACA 
AGTTCTGCCC GGCCATTTGA ACGCAAATTT GAAAGTCCGA AGTTCAACCA TAATCTTCTG 
CCAAGTGAAA CTGTACATAA ACCTGAATTG TCTTCAAAAA CTCCCACTTC CCCAAAAACT 
CTTATGAAAG CTCATAGTTC AACACAGCCT CCAGAGTTTG ACAGTGGAGT TGAAACTTTC 
TCTGTTCACA CAGATAAGCC TAAATATCAA ATGAATAATA TCAGCACCAT GCCTAAAGCT 
GTCCCTGTGA GTCCTTCAGC TGTGGAAGAA GATGAAGATG AGGATGGTCA TACTGTAGTG 
GCTACAGCTC GTGGCATTTT TAACAGCAAT GGTGGTGTGT TGAGTTCCAT AGAAACCGGT 
GTTAGTATAA TTATCCCACA AGGAGCCATT CCTGAAGGAA TTGAGCAAGA AATCTATTTC 
AAAGTCTGCA GAGACAATAG CATTCTCCCA CCTTTAGATA AAGAGAAAGG TGAAACTCTG 
CTGAGCCCCC TAGTGATGTG TGGGCCCCAT GGCCTCAAGT TCCTGAAGCC CGTGGAGCTA 
CGCTTGCCAC ACTGTGCGTC CATGACTCCT GACGGTGACC CTAAAACCTG GCAAAACAAG 
TGTCTTCCTG GAGATCCGAA TTACCTTGTT GGAGCCAACT GTGTTTCTGT CCTGATTGAC 
CACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tjp1 ( XM_006540780.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006540843.1 Mus musculus tight junction protein 1 (Tjp1), transcript variant X3, mRNA.

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CloneID	OMu38770
Clone ID Related Accession (Same CDS sequence)	XM_006540780.4 , XM_006540780.3
Accession Version	XM_006540780.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	tight junction protein ZO-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006540780.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT 
CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA 
CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC 
AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA 
CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA 
AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT 
GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG 
AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG 
TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC 
ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT 
GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT 
GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA 
CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC 
TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT 
GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA 
CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG 
CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT 
AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG 
GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT 
GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT 
CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC 
CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT 
CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT 
GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT 
ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG 
TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA 
ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA 
ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT 
ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC 
ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT 
GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC 
CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT 
CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT 
CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA 
GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT 
AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG 
ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA 
ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT 
CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCCTCATCT 
CCAGTCCCTT ACCTTTCGCC TGAAACAACC CCAGCATCAT CAGCCTCTGC AGTTAATCAT 
AATGTCAGTG TAACTAATGT CAGCCTGGAG GAGCCTGCCC CAGCCCCTCC CACCTCGCAC 
GCATCACAGC CTGGTTGTTT AGGAGCACCA AGTGCTGAGG CAGCTCACGT AGTGCTCAGA 
GGTGAAGGAC CACCATTGCC GCCGCATGCA GACCCAGCAA AGGTGTACAG GAAGGAGCCG 
TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA 
GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA 
AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA 
GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT 
GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT 
CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT 
TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG 
CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC 
AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC 
AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC 
TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT 
TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA 
ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC 
AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT 
GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT 
AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG 
CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT 
GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG 
AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT 
TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAGG GAAAACCCGA AACTGATGCT 
GTGGATAGAT CATTCAGTGA GAAACGTTAT GATCCAGCCC AGGCCACGCC TCCTCCTCCT 
CCGTTGCCCT CACAGTACAG CCAGCCAGCT CCACCTCTGT CCAGCTCTTC TCTCCACATA 
CATTCCAAGG GCGCCCAGGG TGAAGGCAAC TCAGTATCAT TGGATTTTCA GAACTCATAT 
ATGTCCAAAC CAGACCCACC CCCATCTCAG AGTAAACCAG CAACTTTCAG ACCACCAACT 
CGAGAAGACC CCCCTCAGAC TTTCTATCCG CAGAAAAGTT TCCCAGACAA AGCTCCAGTT 
AACGGAGCTG AGCAGACTCA GAAAACCATC ACTCCGGTGT ACAATCGATT CACACCAAAG 
CCGTACACAA GTTCTGCCCG GCCATTTGAA CGCAAATTTG AAAGTCCGAA GTTCAACCAT 
AATCTTCTGC CAAGTGAAAC TGTACATAAA CCTGAATTGT CTTCAAAAAC TCCCACTTCC 
CCAAAAACTC TTATGAAAGC TCATAGTTCA ACACAGCCTC CAGAGTTTGA CAGTGGAGTT 
GAAACTTTCT CTGTTCACAC AGATAAGCCT AAATATCAAA TGAATAATAT CAGCACCATG 
CCTAAAGCTG TCCCTGTGAG TCCTTCAGCT GTGGAAGAAG ATGAAGATGA GGATGGTCAT 
ACTGTAGTGG CTACAGCTCG TGGCATTTTT AACAGCAATG GTGGTGTGTT GAGTTCCATA 
GAAACCGGTG TTAGTATAAT TATCCCACAA GGAGCCATTC CTGAAGGAAT TGAGCAAGAA 
ATCTATTTCA AAGTCTGCAG AGACAATAGC ATTCTCCCAC CTTTAGATAA AGAGAAAGGT 
GAAACTCTGC TGAGCCCCCT AGTGATGTGT GGGCCCCATG GCCTCAAGTT CCTGAAGCCC 
GTGGAGCTAC GCTTGCCACA CTGTGCGTCC ATGACTCCTG ACGGTTGGTC TTTTGCTCTA 
AAATCATCCG ACTCCTCGTC GGGTGACCCT AAAACCTGGC AAAACAAGTG TCTTCCTGGA 
GATCCGAATT ACCTTGTTGG AGCCAACTGT GTTTCTGTCC TGATTGACCA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006540843.1
CDS	72..5648
Translation	MRRLEGLCLRRRRRRRGPARERSGDKMKYQKYLTVLQMAIGVTPSNRGSLLPLKRKLWVTPSSENPHGATSTVSQGKPSLRRIKGRLHRSKSLDSIDFCELTSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSHPDPEPVSDNEDDSYDEEVHDPRAGRGALANRRSEKSWARDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSQSRSPDQRSEPSDHSTQSPQQPSNGSLRSREEERMSKPGAISTPVKHVDDHPPKAVEEVTVEKNEKQTPTLPEPKPVYAQVGQPDVDLPVSPSDGALPNSAHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDHRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQAIHRIDSPGLKPASQQKAEASSPVPYLSPETTPASSASAVNHNVSVTNVSLEEPAPAPPTSHASQPGCLGAPSAEAAHVVLRGEGPPLPPHADPAKVYRKEPYSEEMMRQNHILKQPALGHPGQRPDKEPNLAYEPQLPYIEKQASRDLEQPSYRYEVSSYTDQFSRNYDHRLRFEDRIPTYEDQWSYYDDKQPYQPRPFENQHPRDLDSRQHPEEASERGYFQRFEEPAPLSYDSRTRYEQLPRTSTLRHEEQPAPAYEVHNRYRPEAQPYSSTGPKSSEPKQYFDQYPRSYEQVPPPGFTSKTGHYEPLHGAAVVPPLIPSSQQKPEVLPSATKPQPPPPTLTEEEEDPAMKPQSVLTRVKMFENKRSASLENKKDVNDTASFKPPEVASKPPGASLAGPKPVPQSQFSEHDKTLYRLPEPQKPQVKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFESKPSAHLPAGHHSEPAKPVHSQSQPNFSSYSSKGKPETDAVDRSFSEKRYDPAQATPPPPPLPSQYSQPAPPLSSSSLHIHSKGAQGEGNSVSLDFQNSYMSKPDPPPSQSKPATFRPPTREDPPQTFYPQKSFPDKAPVNGAEQTQKTITPVYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETVHKPELSSKTPTSPKTLMKAHSSTQPPEFDSGVETFSVHTDKPKYQMNNISTMPKAVPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_006540780.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus tight junction protein 1 (Tjp1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006540780.3

ORF Insert Sequence:

ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT 
CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA 
CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC 
AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA 
CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA 
AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT 
GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG 
AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG 
TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC 
ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT 
GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT 
GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA 
CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC 
TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT 
GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA 
CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG 
CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT 
AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG 
GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT 
GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT 
CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC 
CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT 
CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT 
GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT 
ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG 
TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA 
ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA 
ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT 
ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC 
ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT 
GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC 
CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT 
CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT 
CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA 
GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT 
AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG 
ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA 
ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT 
CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCCTCATCT 
CCAGTCCCTT ACCTTTCGCC TGAAACAACC CCAGCATCAT CAGCCTCTGC AGTTAATCAT 
AATGTCAGTG TAACTAATGT CAGCCTGGAG GAGCCTGCCC CAGCCCCTCC CACCTCGCAC 
GCATCACAGC CTGGTTGTTT AGGAGCACCA AGTGCTGAGG CAGCTCACGT AGTGCTCAGA 
GGTGAAGGAC CACCATTGCC GCCGCATGCA GACCCAGCAA AGGTGTACAG GAAGGAGCCG 
TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA 
GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA 
AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA 
GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT 
GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT 
CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT 
TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG 
CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC 
AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC 
AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC 
TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT 
TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA 
ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC 
AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT 
GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT 
AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG 
CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT 
GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG 
AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT 
TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAGG GAAAACCCGA AACTGATGCT 
GTGGATAGAT CATTCAGTGA GAAACGTTAT GATCCAGCCC AGGCCACGCC TCCTCCTCCT 
CCGTTGCCCT CACAGTACAG CCAGCCAGCT CCACCTCTGT CCAGCTCTTC TCTCCACATA 
CATTCCAAGG GCGCCCAGGG TGAAGGCAAC TCAGTATCAT TGGATTTTCA GAACTCATAT 
ATGTCCAAAC CAGACCCACC CCCATCTCAG AGTAAACCAG CAACTTTCAG ACCACCAACT 
CGAGAAGACC CCCCTCAGAC TTTCTATCCG CAGAAAAGTT TCCCAGACAA AGCTCCAGTT 
AACGGAGCTG AGCAGACTCA GAAAACCATC ACTCCGGTGT ACAATCGATT CACACCAAAG 
CCGTACACAA GTTCTGCCCG GCCATTTGAA CGCAAATTTG AAAGTCCGAA GTTCAACCAT 
AATCTTCTGC CAAGTGAAAC TGTACATAAA CCTGAATTGT CTTCAAAAAC TCCCACTTCC 
CCAAAAACTC TTATGAAAGC TCATAGTTCA ACACAGCCTC CAGAGTTTGA CAGTGGAGTT 
GAAACTTTCT CTGTTCACAC AGATAAGCCT AAATATCAAA TGAATAATAT CAGCACCATG 
CCTAAAGCTG TCCCTGTGAG TCCTTCAGCT GTGGAAGAAG ATGAAGATGA GGATGGTCAT 
ACTGTAGTGG CTACAGCTCG TGGCATTTTT AACAGCAATG GTGGTGTGTT GAGTTCCATA 
GAAACCGGTG TTAGTATAAT TATCCCACAA GGAGCCATTC CTGAAGGAAT TGAGCAAGAA 
ATCTATTTCA AAGTCTGCAG AGACAATAGC ATTCTCCCAC CTTTAGATAA AGAGAAAGGT 
GAAACTCTGC TGAGCCCCCT AGTGATGTGT GGGCCCCATG GCCTCAAGTT CCTGAAGCCC 
GTGGAGCTAC GCTTGCCACA CTGTGCGTCC ATGACTCCTG ACGGTTGGTC TTTTGCTCTA 
AAATCATCCG ACTCCTCGTC GGGTGACCCT AAAACCTGGC AAAACAAGTG TCTTCCTGGA 
GATCCGAATT ACCTTGTTGG AGCCAACTGT GTTTCTGTCC TGATTGACCA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tjp1 ( XM_006540781.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006540844.1 Mus musculus tight junction protein 1 (Tjp1), transcript variant X4, mRNA.

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CloneID	OMu38771
Clone ID Related Accession (Same CDS sequence)	XM_006540781.3 , XM_006540781.4
Accession Version	XM_006540781.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5568bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	tight junction protein ZO-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006540781.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT 
CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA 
CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC 
AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA 
CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA 
AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT 
GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG 
AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG 
TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC 
ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT 
GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT 
GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA 
CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC 
TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT 
GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA 
CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG 
CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT 
AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG 
GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT 
GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT 
CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC 
CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT 
CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT 
GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT 
ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG 
TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA 
ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA 
ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT 
ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC 
ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT 
GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC 
CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT 
CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT 
CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA 
GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT 
AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG 
ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA 
ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT 
CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCCTCATCT 
CCAGTCCCTT ACCTTTCGCC TGAAACAACC CCAGCATCAT CAGCCTCTGC AGTTAATCAT 
AATGTCAGTG TAACTAATGT CAGCCTGGAG GAGCCTGCCC CAGCCCCTCC CACCTCGCAC 
GCATCACAGC CTGGTTGTTT AGGAGCACCA AGTGCTGAGG CAGCTCACGT AGTGCTCAGA 
GGTGAAGGAC CACCATTGCC GCCGCATGCA GACCCAGCAA AGGTGTACAG GAAGGAGCCG 
TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA 
GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA 
AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA 
GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT 
GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT 
CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT 
TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG 
CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC 
AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC 
AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC 
TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT 
TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA 
ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC 
AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT 
GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT 
AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG 
CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT 
GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG 
AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT 
TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAAT TTCTTGGCTC TTACACTAGC 
TATGACTACT TGAAAAGGAA CATCAAGTGG GGAAAACCCG AAACTGATGC TGTGGATAGA 
TCATTCAGTG AGAAACGTTA TGATCCAGCC CAGGCCACGC CTCCTCCTCC TCCGTTGCCC 
TCACAGTACA GCCAGCCAGC TCCACCTCTG TCCAGCTCTT CTCTCCACAT ACATTCCAAG 
GGCGCCCAGG GTGAAGGCAA CTCAGTATCA TTGGATTTTC AGAACTCATA TATGTCCAAA 
CCAGACCCAC CCCCATCTCA GAGTAAACCA GCAACTTTCA GACCACCAAC TCGAGAAGAC 
CCCCCTCAGA CTTTCTATCC GCAGAAAAGT TTCCCAGACA AAGCTCCAGT TAACGGAGCT 
GAGCAGACTC AGAAAACCAT CACTCCGGTG TACAATCGAT TCACACCAAA GCCGTACACA 
AGTTCTGCCC GGCCATTTGA ACGCAAATTT GAAAGTCCGA AGTTCAACCA TAATCTTCTG 
CCAAGTGAAA CTGTACATAA ACCTGAATTG TCTTCAAAAA CTCCCACTTC CCCAAAAACT 
CTTATGAAAG CTCATAGTTC AACACAGCCT CCAGAGTTTG ACAGTGGAGT TGAAACTTTC 
TCTGTTCACA CAGATAAGCC TAAATATCAA ATGAATAATA TCAGCACCAT GCCTAAAGCT 
GTCCCTGTGA GTCCTTCAGC TGTGGAAGAA GATGAAGATG AGGATGGTCA TACTGTAGTG 
GCTACAGCTC GTGGCATTTT TAACAGCAAT GGTGGTGTGT TGAGTTCCAT AGAAACCGGT 
GTTAGTATAA TTATCCCACA AGGAGCCATT CCTGAAGGAA TTGAGCAAGA AATCTATTTC 
AAAGTCTGCA GAGACAATAG CATTCTCCCA CCTTTAGATA AAGAGAAAGG TGAAACTCTG 
CTGAGCCCCC TAGTGATGTG TGGGCCCCAT GGCCTCAAGT TCCTGAAGCC CGTGGAGCTA 
CGCTTGCCAC ACTGTGACCC TAAAACCTGG CAAAACAAGT GTCTTCCTGG AGATCCGAAT 
TACCTTGTTG GAGCCAACTG TGTTTCTGTC CTGATTGACC ACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006540844.1
CDS	73..5640
Translation	MRRLEGLCLRRRRRRRGPARERSGDKMKYQKYLTVLQMAIGVTPSNRGSLLPLKRKLWVTPSSENPHGATSTVSQGKPSLRRIKGRLHRSKSLDSIDFCELTSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSHPDPEPVSDNEDDSYDEEVHDPRAGRGALANRRSEKSWARDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSQSRSPDQRSEPSDHSTQSPQQPSNGSLRSREEERMSKPGAISTPVKHVDDHPPKAVEEVTVEKNEKQTPTLPEPKPVYAQVGQPDVDLPVSPSDGALPNSAHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDHRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQAIHRIDSPGLKPASQQKAEASSPVPYLSPETTPASSASAVNHNVSVTNVSLEEPAPAPPTSHASQPGCLGAPSAEAAHVVLRGEGPPLPPHADPAKVYRKEPYSEEMMRQNHILKQPALGHPGQRPDKEPNLAYEPQLPYIEKQASRDLEQPSYRYEVSSYTDQFSRNYDHRLRFEDRIPTYEDQWSYYDDKQPYQPRPFENQHPRDLDSRQHPEEASERGYFQRFEEPAPLSYDSRTRYEQLPRTSTLRHEEQPAPAYEVHNRYRPEAQPYSSTGPKSSEPKQYFDQYPRSYEQVPPPGFTSKTGHYEPLHGAAVVPPLIPSSQQKPEVLPSATKPQPPPPTLTEEEEDPAMKPQSVLTRVKMFENKRSASLENKKDVNDTASFKPPEVASKPPGASLAGPKPVPQSQFSEHDKTLYRLPEPQKPQVKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFESKPSAHLPAGHHSEPAKPVHSQSQPNFSSYSSKFLGSYTSYDYLKRNIKWGKPETDAVDRSFSEKRYDPAQATPPPPPLPSQYSQPAPPLSSSSLHIHSKGAQGEGNSVSLDFQNSYMSKPDPPPSQSKPATFRPPTREDPPQTFYPQKSFPDKAPVNGAEQTQKTITPVYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETVHKPELSSKTPTSPKTLMKAHSSTQPPEFDSGVETFSVHTDKPKYQMNNISTMPKAVPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_006540781.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus tight junction protein 1 (Tjp1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006540781.3

ORF Insert Sequence:

ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT 
CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA 
CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC 
AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA 
CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA 
AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT 
GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG 
AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG 
TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC 
ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT 
GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT 
GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA 
CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC 
TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT 
GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA 
CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG 
CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT 
AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG 
GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT 
GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT 
CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC 
CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT 
CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT 
GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT 
ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG 
TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA 
ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA 
ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT 
ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC 
ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT 
GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC 
CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT 
CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT 
CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA 
GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT 
AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG 
ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA 
ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT 
CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCCTCATCT 
CCAGTCCCTT ACCTTTCGCC TGAAACAACC CCAGCATCAT CAGCCTCTGC AGTTAATCAT 
AATGTCAGTG TAACTAATGT CAGCCTGGAG GAGCCTGCCC CAGCCCCTCC CACCTCGCAC 
GCATCACAGC CTGGTTGTTT AGGAGCACCA AGTGCTGAGG CAGCTCACGT AGTGCTCAGA 
GGTGAAGGAC CACCATTGCC GCCGCATGCA GACCCAGCAA AGGTGTACAG GAAGGAGCCG 
TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA 
GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA 
AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA 
GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT 
GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT 
CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT 
TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG 
CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC 
AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC 
AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC 
TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT 
TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA 
ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC 
AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT 
GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT 
AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG 
CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT 
GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG 
AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT 
TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAAT TTCTTGGCTC TTACACTAGC 
TATGACTACT TGAAAAGGAA CATCAAGTGG GGAAAACCCG AAACTGATGC TGTGGATAGA 
TCATTCAGTG AGAAACGTTA TGATCCAGCC CAGGCCACGC CTCCTCCTCC TCCGTTGCCC 
TCACAGTACA GCCAGCCAGC TCCACCTCTG TCCAGCTCTT CTCTCCACAT ACATTCCAAG 
GGCGCCCAGG GTGAAGGCAA CTCAGTATCA TTGGATTTTC AGAACTCATA TATGTCCAAA 
CCAGACCCAC CCCCATCTCA GAGTAAACCA GCAACTTTCA GACCACCAAC TCGAGAAGAC 
CCCCCTCAGA CTTTCTATCC GCAGAAAAGT TTCCCAGACA AAGCTCCAGT TAACGGAGCT 
GAGCAGACTC AGAAAACCAT CACTCCGGTG TACAATCGAT TCACACCAAA GCCGTACACA 
AGTTCTGCCC GGCCATTTGA ACGCAAATTT GAAAGTCCGA AGTTCAACCA TAATCTTCTG 
CCAAGTGAAA CTGTACATAA ACCTGAATTG TCTTCAAAAA CTCCCACTTC CCCAAAAACT 
CTTATGAAAG CTCATAGTTC AACACAGCCT CCAGAGTTTG ACAGTGGAGT TGAAACTTTC 
TCTGTTCACA CAGATAAGCC TAAATATCAA ATGAATAATA TCAGCACCAT GCCTAAAGCT 
GTCCCTGTGA GTCCTTCAGC TGTGGAAGAA GATGAAGATG AGGATGGTCA TACTGTAGTG 
GCTACAGCTC GTGGCATTTT TAACAGCAAT GGTGGTGTGT TGAGTTCCAT AGAAACCGGT 
GTTAGTATAA TTATCCCACA AGGAGCCATT CCTGAAGGAA TTGAGCAAGA AATCTATTTC 
AAAGTCTGCA GAGACAATAG CATTCTCCCA CCTTTAGATA AAGAGAAAGG TGAAACTCTG 
CTGAGCCCCC TAGTGATGTG TGGGCCCCAT GGCCTCAAGT TCCTGAAGCC CGTGGAGCTA 
CGCTTGCCAC ACTGTGACCC TAAAACCTGG CAAAACAAGT GTCTTCCTGG AGATCCGAAT 
TACCTTGTTG GAGCCAACTG TGTTTCTGTC CTGATTGACC ACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tjp1 ( XM_006540782.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006540845.1 Mus musculus tight junction protein 1 (Tjp1), transcript variant X6, mRNA.

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CloneID	OMu38772
Clone ID Related Accession (Same CDS sequence)	XM_006540782.4 , XM_006540782.3
Accession Version	XM_006540782.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5517bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	tight junction protein ZO-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006540782.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT 
CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA 
CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC 
AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA 
CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA 
AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT 
GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG 
AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG 
TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC 
ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT 
GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT 
GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA 
CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC 
TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT 
GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA 
CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG 
CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT 
AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG 
GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT 
GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT 
CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC 
CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT 
CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT 
GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT 
ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG 
TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA 
ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA 
ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT 
ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC 
ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT 
GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC 
CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT 
CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT 
CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA 
GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT 
AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG 
ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA 
ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT 
CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCCTCATCT 
CCAGTCCCTT ACCTTTCGCC TGAAACAACC CCAGCATCAT CAGCCTCTGC AGTTAATCAT 
AATGTCAGTG TAACTAATGT CAGCCTGGAG GAGCCTGCCC CAGCCCCTCC CACCTCGCAC 
GCATCACAGC CTGGTTGTTT AGGAGCACCA AGTGCTGAGG CAGCTCACGT AGTGCTCAGA 
GGTGAAGGAC CACCATTGCC GCCGCATGCA GACCCAGCAA AGGTGTACAG GAAGGAGCCG 
TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA 
GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA 
AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA 
GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT 
GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT 
CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT 
TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG 
CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC 
AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC 
AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC 
TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT 
TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA 
ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC 
AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT 
GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT 
AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG 
CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT 
GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG 
AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT 
TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAGG GAAAACCCGA AACTGATGCT 
GTGGATAGAT CATTCAGTGA GAAACGTTAT GATCCAGCCC AGGCCACGCC TCCTCCTCCT 
CCGTTGCCCT CACAGTACAG CCAGCCAGCT CCACCTCTGT CCAGCTCTTC TCTCCACATA 
CATTCCAAGG GCGCCCAGGG TGAAGGCAAC TCAGTATCAT TGGATTTTCA GAACTCATAT 
ATGTCCAAAC CAGACCCACC CCCATCTCAG AGTAAACCAG CAACTTTCAG ACCACCAACT 
CGAGAAGACC CCCCTCAGAC TTTCTATCCG CAGAAAAGTT TCCCAGACAA AGCTCCAGTT 
AACGGAGCTG AGCAGACTCA GAAAACCATC ACTCCGGTGT ACAATCGATT CACACCAAAG 
CCGTACACAA GTTCTGCCCG GCCATTTGAA CGCAAATTTG AAAGTCCGAA GTTCAACCAT 
AATCTTCTGC CAAGTGAAAC TGTACATAAA CCTGAATTGT CTTCAAAAAC TCCCACTTCC 
CCAAAAACTC TTATGAAAGC TCATAGTTCA ACACAGCCTC CAGAGTTTGA CAGTGGAGTT 
GAAACTTTCT CTGTTCACAC AGATAAGCCT AAATATCAAA TGAATAATAT CAGCACCATG 
CCTAAAGCTG TCCCTGTGAG TCCTTCAGCT GTGGAAGAAG ATGAAGATGA GGATGGTCAT 
ACTGTAGTGG CTACAGCTCG TGGCATTTTT AACAGCAATG GTGGTGTGTT GAGTTCCATA 
GAAACCGGTG TTAGTATAAT TATCCCACAA GGAGCCATTC CTGAAGGAAT TGAGCAAGAA 
ATCTATTTCA AAGTCTGCAG AGACAATAGC ATTCTCCCAC CTTTAGATAA AGAGAAAGGT 
GAAACTCTGC TGAGCCCCCT AGTGATGTGT GGGCCCCATG GCCTCAAGTT CCTGAAGCCC 
GTGGAGCTAC GCTTGCCACA CTGTGACCCT AAAACCTGGC AAAACAAGTG TCTTCCTGGA 
GATCCGAATT ACCTTGTTGG AGCCAACTGT GTTTCTGTCC TGATTGACCA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006540845.1
CDS	72..5588
Translation	MRRLEGLCLRRRRRRRGPARERSGDKMKYQKYLTVLQMAIGVTPSNRGSLLPLKRKLWVTPSSENPHGATSTVSQGKPSLRRIKGRLHRSKSLDSIDFCELTSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSHPDPEPVSDNEDDSYDEEVHDPRAGRGALANRRSEKSWARDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSQSRSPDQRSEPSDHSTQSPQQPSNGSLRSREEERMSKPGAISTPVKHVDDHPPKAVEEVTVEKNEKQTPTLPEPKPVYAQVGQPDVDLPVSPSDGALPNSAHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDHRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQAIHRIDSPGLKPASQQKAEASSPVPYLSPETTPASSASAVNHNVSVTNVSLEEPAPAPPTSHASQPGCLGAPSAEAAHVVLRGEGPPLPPHADPAKVYRKEPYSEEMMRQNHILKQPALGHPGQRPDKEPNLAYEPQLPYIEKQASRDLEQPSYRYEVSSYTDQFSRNYDHRLRFEDRIPTYEDQWSYYDDKQPYQPRPFENQHPRDLDSRQHPEEASERGYFQRFEEPAPLSYDSRTRYEQLPRTSTLRHEEQPAPAYEVHNRYRPEAQPYSSTGPKSSEPKQYFDQYPRSYEQVPPPGFTSKTGHYEPLHGAAVVPPLIPSSQQKPEVLPSATKPQPPPPTLTEEEEDPAMKPQSVLTRVKMFENKRSASLENKKDVNDTASFKPPEVASKPPGASLAGPKPVPQSQFSEHDKTLYRLPEPQKPQVKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFESKPSAHLPAGHHSEPAKPVHSQSQPNFSSYSSKGKPETDAVDRSFSEKRYDPAQATPPPPPLPSQYSQPAPPLSSSSLHIHSKGAQGEGNSVSLDFQNSYMSKPDPPPSQSKPATFRPPTREDPPQTFYPQKSFPDKAPVNGAEQTQKTITPVYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETVHKPELSSKTPTSPKTLMKAHSSTQPPEFDSGVETFSVHTDKPKYQMNNISTMPKAVPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_006540782.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus tight junction protein 1 (Tjp1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006540782.3

ORF Insert Sequence:

ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT 
CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA 
CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC 
AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA 
CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA 
AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT 
GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG 
AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG 
TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC 
ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT 
GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT 
GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA 
CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC 
TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT 
GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA 
CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG 
CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT 
AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG 
GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT 
GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT 
CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC 
CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT 
CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT 
GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT 
ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG 
TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA 
ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA 
ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT 
ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC 
ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT 
GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC 
CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT 
CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT 
CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA 
GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT 
AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG 
ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA 
ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT 
CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCCTCATCT 
CCAGTCCCTT ACCTTTCGCC TGAAACAACC CCAGCATCAT CAGCCTCTGC AGTTAATCAT 
AATGTCAGTG TAACTAATGT CAGCCTGGAG GAGCCTGCCC CAGCCCCTCC CACCTCGCAC 
GCATCACAGC CTGGTTGTTT AGGAGCACCA AGTGCTGAGG CAGCTCACGT AGTGCTCAGA 
GGTGAAGGAC CACCATTGCC GCCGCATGCA GACCCAGCAA AGGTGTACAG GAAGGAGCCG 
TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA 
GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA 
AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA 
GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT 
GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT 
CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT 
TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG 
CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC 
AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC 
AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC 
TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT 
TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA 
ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC 
AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT 
GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT 
AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG 
CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT 
GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG 
AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT 
TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAGG GAAAACCCGA AACTGATGCT 
GTGGATAGAT CATTCAGTGA GAAACGTTAT GATCCAGCCC AGGCCACGCC TCCTCCTCCT 
CCGTTGCCCT CACAGTACAG CCAGCCAGCT CCACCTCTGT CCAGCTCTTC TCTCCACATA 
CATTCCAAGG GCGCCCAGGG TGAAGGCAAC TCAGTATCAT TGGATTTTCA GAACTCATAT 
ATGTCCAAAC CAGACCCACC CCCATCTCAG AGTAAACCAG CAACTTTCAG ACCACCAACT 
CGAGAAGACC CCCCTCAGAC TTTCTATCCG CAGAAAAGTT TCCCAGACAA AGCTCCAGTT 
AACGGAGCTG AGCAGACTCA GAAAACCATC ACTCCGGTGT ACAATCGATT CACACCAAAG 
CCGTACACAA GTTCTGCCCG GCCATTTGAA CGCAAATTTG AAAGTCCGAA GTTCAACCAT 
AATCTTCTGC CAAGTGAAAC TGTACATAAA CCTGAATTGT CTTCAAAAAC TCCCACTTCC 
CCAAAAACTC TTATGAAAGC TCATAGTTCA ACACAGCCTC CAGAGTTTGA CAGTGGAGTT 
GAAACTTTCT CTGTTCACAC AGATAAGCCT AAATATCAAA TGAATAATAT CAGCACCATG 
CCTAAAGCTG TCCCTGTGAG TCCTTCAGCT GTGGAAGAAG ATGAAGATGA GGATGGTCAT 
ACTGTAGTGG CTACAGCTCG TGGCATTTTT AACAGCAATG GTGGTGTGTT GAGTTCCATA 
GAAACCGGTG TTAGTATAAT TATCCCACAA GGAGCCATTC CTGAAGGAAT TGAGCAAGAA 
ATCTATTTCA AAGTCTGCAG AGACAATAGC ATTCTCCCAC CTTTAGATAA AGAGAAAGGT 
GAAACTCTGC TGAGCCCCCT AGTGATGTGT GGGCCCCATG GCCTCAAGTT CCTGAAGCCC 
GTGGAGCTAC GCTTGCCACA CTGTGACCCT AAAACCTGGC AAAACAAGTG TCTTCCTGGA 
GATCCGAATT ACCTTGTTGG AGCCAACTGT GTTTCTGTCC TGATTGACCA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tjp1 ( XM_006540783.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006540846.1 Mus musculus tight junction protein 1 (Tjp1), transcript variant X7, mRNA.

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CloneID	OMu38773
Clone ID Related Accession (Same CDS sequence)	XM_006540783.4 , XM_006540783.3
Accession Version	XM_006540783.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5388bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	tight junction protein ZO-1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006540783.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT 
CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA 
CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC 
AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA 
CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA 
AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT 
GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG 
AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG 
TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC 
ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT 
GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT 
GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA 
CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC 
TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT 
GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA 
CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG 
CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT 
AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG 
GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT 
GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT 
CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC 
CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT 
CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT 
GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT 
ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG 
TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA 
ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA 
ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT 
ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC 
ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT 
GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC 
CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT 
CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT 
CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA 
GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT 
AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG 
ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA 
ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT 
CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGGTGTACAG GAAGGAGCCG 
TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA 
GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA 
AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA 
GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT 
GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT 
CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT 
TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG 
CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC 
AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC 
AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC 
TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT 
TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA 
ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC 
AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT 
GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT 
AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG 
CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT 
GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG 
AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT 
TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAAT TTCTTGGCTC TTACACTAGC 
TATGACTACT TGAAAAGGAA CATCAAGTGG GGAAAACCCG AAACTGATGC TGTGGATAGA 
TCATTCAGTG AGAAACGTTA TGATCCAGCC CAGGCCACGC CTCCTCCTCC TCCGTTGCCC 
TCACAGTACA GCCAGCCAGC TCCACCTCTG TCCAGCTCTT CTCTCCACAT ACATTCCAAG 
GGCGCCCAGG GTGAAGGCAA CTCAGTATCA TTGGATTTTC AGAACTCATA TATGTCCAAA 
CCAGACCCAC CCCCATCTCA GAGTAAACCA GCAACTTTCA GACCACCAAC TCGAGAAGAC 
CCCCCTCAGA CTTTCTATCC GCAGAAAAGT TTCCCAGACA AAGCTCCAGT TAACGGAGCT 
GAGCAGACTC AGAAAACCAT CACTCCGGTG TACAATCGAT TCACACCAAA GCCGTACACA 
AGTTCTGCCC GGCCATTTGA ACGCAAATTT GAAAGTCCGA AGTTCAACCA TAATCTTCTG 
CCAAGTGAAA CTGTACATAA ACCTGAATTG TCTTCAAAAA CTCCCACTTC CCCAAAAACT 
CTTATGAAAG CTCATAGTTC AACACAGCCT CCAGAGTTTG ACAGTGGAGT TGAAACTTTC 
TCTGTTCACA CAGATAAGCC TAAATATCAA ATGAATAATA TCAGCACCAT GCCTAAAGCT 
GTCCCTGTGA GTCCTTCAGC TGTGGAAGAA GATGAAGATG AGGATGGTCA TACTGTAGTG 
GCTACAGCTC GTGGCATTTT TAACAGCAAT GGTGGTGTGT TGAGTTCCAT AGAAACCGGT 
GTTAGTATAA TTATCCCACA AGGAGCCATT CCTGAAGGAA TTGAGCAAGA AATCTATTTC 
AAAGTCTGCA GAGACAATAG CATTCTCCCA CCTTTAGATA AAGAGAAAGG TGAAACTCTG 
CTGAGCCCCC TAGTGATGTG TGGGCCCCAT GGCCTCAAGT TCCTGAAGCC CGTGGAGCTA 
CGCTTGCCAC ACTGTGCGTC CATGACTCCT GACGGTTGGT CTTTTGCTCT AAAATCATCC 
GACTCCTCGT CGGGTGACCC TAAAACCTGG CAAAACAAGT GTCTTCCTGG AGATCCGAAT 
TACCTTGTTG GAGCCAACTG TGTTTCTGTC CTGATTGACC ACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006540846.1
CDS	72..5459
Translation	MRRLEGLCLRRRRRRRGPARERSGDKMKYQKYLTVLQMAIGVTPSNRGSLLPLKRKLWVTPSSENPHGATSTVSQGKPSLRRIKGRLHRSKSLDSIDFCELTSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSHPDPEPVSDNEDDSYDEEVHDPRAGRGALANRRSEKSWARDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSQSRSPDQRSEPSDHSTQSPQQPSNGSLRSREEERMSKPGAISTPVKHVDDHPPKAVEEVTVEKNEKQTPTLPEPKPVYAQVGQPDVDLPVSPSDGALPNSAHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDHRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQAIHRIDSPGLKPASQQVYRKEPYSEEMMRQNHILKQPALGHPGQRPDKEPNLAYEPQLPYIEKQASRDLEQPSYRYEVSSYTDQFSRNYDHRLRFEDRIPTYEDQWSYYDDKQPYQPRPFENQHPRDLDSRQHPEEASERGYFQRFEEPAPLSYDSRTRYEQLPRTSTLRHEEQPAPAYEVHNRYRPEAQPYSSTGPKSSEPKQYFDQYPRSYEQVPPPGFTSKTGHYEPLHGAAVVPPLIPSSQQKPEVLPSATKPQPPPPTLTEEEEDPAMKPQSVLTRVKMFENKRSASLENKKDVNDTASFKPPEVASKPPGASLAGPKPVPQSQFSEHDKTLYRLPEPQKPQVKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFESKPSAHLPAGHHSEPAKPVHSQSQPNFSSYSSKFLGSYTSYDYLKRNIKWGKPETDAVDRSFSEKRYDPAQATPPPPPLPSQYSQPAPPLSSSSLHIHSKGAQGEGNSVSLDFQNSYMSKPDPPPSQSKPATFRPPTREDPPQTFYPQKSFPDKAPVNGAEQTQKTITPVYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETVHKPELSSKTPTSPKTLMKAHSSTQPPEFDSGVETFSVHTDKPKYQMNNISTMPKAVPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_006540783.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus tight junction protein 1 (Tjp1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006540783.3

ORF Insert Sequence:

ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT 
CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA 
CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC 
AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA 
CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA 
AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT 
GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG 
AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG 
TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC 
ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT 
GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT 
GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA 
CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC 
TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT 
GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA 
CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG 
CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT 
AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG 
GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT 
GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT 
CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC 
CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT 
CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT 
GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT 
ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG 
TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA 
ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA 
ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT 
ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC 
ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT 
GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC 
CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT 
CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT 
CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA 
GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT 
AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG 
ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA 
ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT 
CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGGTGTACAG GAAGGAGCCG 
TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA 
GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA 
AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA 
GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT 
GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT 
CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT 
TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG 
CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC 
AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC 
AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC 
TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT 
TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA 
ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC 
AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT 
GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT 
AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG 
CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT 
GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG 
AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT 
TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAAT TTCTTGGCTC TTACACTAGC 
TATGACTACT TGAAAAGGAA CATCAAGTGG GGAAAACCCG AAACTGATGC TGTGGATAGA 
TCATTCAGTG AGAAACGTTA TGATCCAGCC CAGGCCACGC CTCCTCCTCC TCCGTTGCCC 
TCACAGTACA GCCAGCCAGC TCCACCTCTG TCCAGCTCTT CTCTCCACAT ACATTCCAAG 
GGCGCCCAGG GTGAAGGCAA CTCAGTATCA TTGGATTTTC AGAACTCATA TATGTCCAAA 
CCAGACCCAC CCCCATCTCA GAGTAAACCA GCAACTTTCA GACCACCAAC TCGAGAAGAC 
CCCCCTCAGA CTTTCTATCC GCAGAAAAGT TTCCCAGACA AAGCTCCAGT TAACGGAGCT 
GAGCAGACTC AGAAAACCAT CACTCCGGTG TACAATCGAT TCACACCAAA GCCGTACACA 
AGTTCTGCCC GGCCATTTGA ACGCAAATTT GAAAGTCCGA AGTTCAACCA TAATCTTCTG 
CCAAGTGAAA CTGTACATAA ACCTGAATTG TCTTCAAAAA CTCCCACTTC CCCAAAAACT 
CTTATGAAAG CTCATAGTTC AACACAGCCT CCAGAGTTTG ACAGTGGAGT TGAAACTTTC 
TCTGTTCACA CAGATAAGCC TAAATATCAA ATGAATAATA TCAGCACCAT GCCTAAAGCT 
GTCCCTGTGA GTCCTTCAGC TGTGGAAGAA GATGAAGATG AGGATGGTCA TACTGTAGTG 
GCTACAGCTC GTGGCATTTT TAACAGCAAT GGTGGTGTGT TGAGTTCCAT AGAAACCGGT 
GTTAGTATAA TTATCCCACA AGGAGCCATT CCTGAAGGAA TTGAGCAAGA AATCTATTTC 
AAAGTCTGCA GAGACAATAG CATTCTCCCA CCTTTAGATA AAGAGAAAGG TGAAACTCTG 
CTGAGCCCCC TAGTGATGTG TGGGCCCCAT GGCCTCAAGT TCCTGAAGCC CGTGGAGCTA 
CGCTTGCCAC ACTGTGCGTC CATGACTCCT GACGGTTGGT CTTTTGCTCT AAAATCATCC 
GACTCCTCGT CGGGTGACCC TAAAACCTGG CAAAACAAGT GTCTTCCTGG AGATCCGAAT 
TACCTTGTTG GAGCCAACTG TGTTTCTGTC CTGATTGACC ACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tjp1 ( XM_006540785.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006540848.1 Mus musculus tight junction protein 1 (Tjp1), transcript variant X9, mRNA.

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CloneID	OMu38775
Clone ID Related Accession (Same CDS sequence)	XM_006540785.3 , XM_006540785.4
Accession Version	XM_006540785.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5337bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	tight junction protein ZO-1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006540785.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT 
CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA 
CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC 
AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA 
CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA 
AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT 
GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG 
AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG 
TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC 
ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT 
GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT 
GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA 
CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC 
TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT 
GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA 
CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG 
CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT 
AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG 
GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT 
GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT 
CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC 
CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT 
CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT 
GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT 
ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG 
TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA 
ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA 
ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT 
ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC 
ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT 
GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC 
CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT 
CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT 
CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA 
GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT 
AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG 
ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA 
ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT 
CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGGTGTACAG GAAGGAGCCG 
TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA 
GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA 
AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA 
GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT 
GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT 
CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT 
TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG 
CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC 
AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC 
AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC 
TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT 
TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA 
ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC 
AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT 
GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT 
AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG 
CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT 
GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG 
AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT 
TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAGG GAAAACCCGA AACTGATGCT 
GTGGATAGAT CATTCAGTGA GAAACGTTAT GATCCAGCCC AGGCCACGCC TCCTCCTCCT 
CCGTTGCCCT CACAGTACAG CCAGCCAGCT CCACCTCTGT CCAGCTCTTC TCTCCACATA 
CATTCCAAGG GCGCCCAGGG TGAAGGCAAC TCAGTATCAT TGGATTTTCA GAACTCATAT 
ATGTCCAAAC CAGACCCACC CCCATCTCAG AGTAAACCAG CAACTTTCAG ACCACCAACT 
CGAGAAGACC CCCCTCAGAC TTTCTATCCG CAGAAAAGTT TCCCAGACAA AGCTCCAGTT 
AACGGAGCTG AGCAGACTCA GAAAACCATC ACTCCGGTGT ACAATCGATT CACACCAAAG 
CCGTACACAA GTTCTGCCCG GCCATTTGAA CGCAAATTTG AAAGTCCGAA GTTCAACCAT 
AATCTTCTGC CAAGTGAAAC TGTACATAAA CCTGAATTGT CTTCAAAAAC TCCCACTTCC 
CCAAAAACTC TTATGAAAGC TCATAGTTCA ACACAGCCTC CAGAGTTTGA CAGTGGAGTT 
GAAACTTTCT CTGTTCACAC AGATAAGCCT AAATATCAAA TGAATAATAT CAGCACCATG 
CCTAAAGCTG TCCCTGTGAG TCCTTCAGCT GTGGAAGAAG ATGAAGATGA GGATGGTCAT 
ACTGTAGTGG CTACAGCTCG TGGCATTTTT AACAGCAATG GTGGTGTGTT GAGTTCCATA 
GAAACCGGTG TTAGTATAAT TATCCCACAA GGAGCCATTC CTGAAGGAAT TGAGCAAGAA 
ATCTATTTCA AAGTCTGCAG AGACAATAGC ATTCTCCCAC CTTTAGATAA AGAGAAAGGT 
GAAACTCTGC TGAGCCCCCT AGTGATGTGT GGGCCCCATG GCCTCAAGTT CCTGAAGCCC 
GTGGAGCTAC GCTTGCCACA CTGTGCGTCC ATGACTCCTG ACGGTTGGTC TTTTGCTCTA 
AAATCATCCG ACTCCTCGTC GGGTGACCCT AAAACCTGGC AAAACAAGTG TCTTCCTGGA 
GATCCGAATT ACCTTGTTGG AGCCAACTGT GTTTCTGTCC TGATTGACCA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006540848.1
CDS	72..5408
Translation	MRRLEGLCLRRRRRRRGPARERSGDKMKYQKYLTVLQMAIGVTPSNRGSLLPLKRKLWVTPSSENPHGATSTVSQGKPSLRRIKGRLHRSKSLDSIDFCELTSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSHPDPEPVSDNEDDSYDEEVHDPRAGRGALANRRSEKSWARDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSQSRSPDQRSEPSDHSTQSPQQPSNGSLRSREEERMSKPGAISTPVKHVDDHPPKAVEEVTVEKNEKQTPTLPEPKPVYAQVGQPDVDLPVSPSDGALPNSAHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDHRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQAIHRIDSPGLKPASQQVYRKEPYSEEMMRQNHILKQPALGHPGQRPDKEPNLAYEPQLPYIEKQASRDLEQPSYRYEVSSYTDQFSRNYDHRLRFEDRIPTYEDQWSYYDDKQPYQPRPFENQHPRDLDSRQHPEEASERGYFQRFEEPAPLSYDSRTRYEQLPRTSTLRHEEQPAPAYEVHNRYRPEAQPYSSTGPKSSEPKQYFDQYPRSYEQVPPPGFTSKTGHYEPLHGAAVVPPLIPSSQQKPEVLPSATKPQPPPPTLTEEEEDPAMKPQSVLTRVKMFENKRSASLENKKDVNDTASFKPPEVASKPPGASLAGPKPVPQSQFSEHDKTLYRLPEPQKPQVKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFESKPSAHLPAGHHSEPAKPVHSQSQPNFSSYSSKGKPETDAVDRSFSEKRYDPAQATPPPPPLPSQYSQPAPPLSSSSLHIHSKGAQGEGNSVSLDFQNSYMSKPDPPPSQSKPATFRPPTREDPPQTFYPQKSFPDKAPVNGAEQTQKTITPVYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETVHKPELSSKTPTSPKTLMKAHSSTQPPEFDSGVETFSVHTDKPKYQMNNISTMPKAVPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_006540785.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus tight junction protein 1 (Tjp1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006540785.3

ORF Insert Sequence:

ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT 
CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA 
CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC 
AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA 
CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA 
AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT 
GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG 
AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG 
TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC 
ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT 
GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT 
GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA 
CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC 
TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT 
GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA 
CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG 
CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT 
AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG 
GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT 
GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT 
CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC 
CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT 
CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT 
GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT 
ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG 
TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA 
ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA 
ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT 
ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC 
ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT 
GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC 
CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT 
CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT 
CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA 
GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT 
AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG 
ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA 
ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT 
CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGGTGTACAG GAAGGAGCCG 
TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA 
GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA 
AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA 
GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT 
GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT 
CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT 
TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG 
CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC 
AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC 
AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC 
TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT 
TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA 
ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC 
AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT 
GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT 
AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG 
CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT 
GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG 
AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT 
TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAGG GAAAACCCGA AACTGATGCT 
GTGGATAGAT CATTCAGTGA GAAACGTTAT GATCCAGCCC AGGCCACGCC TCCTCCTCCT 
CCGTTGCCCT CACAGTACAG CCAGCCAGCT CCACCTCTGT CCAGCTCTTC TCTCCACATA 
CATTCCAAGG GCGCCCAGGG TGAAGGCAAC TCAGTATCAT TGGATTTTCA GAACTCATAT 
ATGTCCAAAC CAGACCCACC CCCATCTCAG AGTAAACCAG CAACTTTCAG ACCACCAACT 
CGAGAAGACC CCCCTCAGAC TTTCTATCCG CAGAAAAGTT TCCCAGACAA AGCTCCAGTT 
AACGGAGCTG AGCAGACTCA GAAAACCATC ACTCCGGTGT ACAATCGATT CACACCAAAG 
CCGTACACAA GTTCTGCCCG GCCATTTGAA CGCAAATTTG AAAGTCCGAA GTTCAACCAT 
AATCTTCTGC CAAGTGAAAC TGTACATAAA CCTGAATTGT CTTCAAAAAC TCCCACTTCC 
CCAAAAACTC TTATGAAAGC TCATAGTTCA ACACAGCCTC CAGAGTTTGA CAGTGGAGTT 
GAAACTTTCT CTGTTCACAC AGATAAGCCT AAATATCAAA TGAATAATAT CAGCACCATG 
CCTAAAGCTG TCCCTGTGAG TCCTTCAGCT GTGGAAGAAG ATGAAGATGA GGATGGTCAT 
ACTGTAGTGG CTACAGCTCG TGGCATTTTT AACAGCAATG GTGGTGTGTT GAGTTCCATA 
GAAACCGGTG TTAGTATAAT TATCCCACAA GGAGCCATTC CTGAAGGAAT TGAGCAAGAA 
ATCTATTTCA AAGTCTGCAG AGACAATAGC ATTCTCCCAC CTTTAGATAA AGAGAAAGGT 
GAAACTCTGC TGAGCCCCCT AGTGATGTGT GGGCCCCATG GCCTCAAGTT CCTGAAGCCC 
GTGGAGCTAC GCTTGCCACA CTGTGCGTCC ATGACTCCTG ACGGTTGGTC TTTTGCTCTA 
AAATCATCCG ACTCCTCGTC GGGTGACCCT AAAACCTGGC AAAACAAGTG TCTTCCTGGA 
GATCCGAATT ACCTTGTTGG AGCCAACTGT GTTTCTGTCC TGATTGACCA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tjp1 ( XM_006540786.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006540849.1 Mus musculus tight junction protein 1 (Tjp1), transcript variant X10, mRNA.

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CloneID	OMu38776
Clone ID Related Accession (Same CDS sequence)	XM_006540786.4 , XM_006540786.3
Accession Version	XM_006540786.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5277bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	tight junction protein ZO-1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006540786.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT 
CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA 
CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC 
AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA 
CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA 
AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT 
GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG 
AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG 
TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC 
ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT 
GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT 
GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA 
CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC 
TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT 
GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA 
CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG 
CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT 
AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG 
GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT 
GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT 
CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC 
CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT 
CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT 
GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT 
ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG 
TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA 
ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA 
ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT 
ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC 
ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT 
GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC 
CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT 
CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT 
CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA 
GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT 
AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG 
ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA 
ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT 
CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGGTGTACAG GAAGGAGCCG 
TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA 
GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA 
AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA 
GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT 
GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT 
CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT 
TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG 
CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC 
AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC 
AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC 
TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT 
TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA 
ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC 
AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT 
GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT 
AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG 
CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT 
GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG 
AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT 
TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAGG GAAAACCCGA AACTGATGCT 
GTGGATAGAT CATTCAGTGA GAAACGTTAT GATCCAGCCC AGGCCACGCC TCCTCCTCCT 
CCGTTGCCCT CACAGTACAG CCAGCCAGCT CCACCTCTGT CCAGCTCTTC TCTCCACATA 
CATTCCAAGG GCGCCCAGGG TGAAGGCAAC TCAGTATCAT TGGATTTTCA GAACTCATAT 
ATGTCCAAAC CAGACCCACC CCCATCTCAG AGTAAACCAG CAACTTTCAG ACCACCAACT 
CGAGAAGACC CCCCTCAGAC TTTCTATCCG CAGAAAAGTT TCCCAGACAA AGCTCCAGTT 
AACGGAGCTG AGCAGACTCA GAAAACCATC ACTCCGGTGT ACAATCGATT CACACCAAAG 
CCGTACACAA GTTCTGCCCG GCCATTTGAA CGCAAATTTG AAAGTCCGAA GTTCAACCAT 
AATCTTCTGC CAAGTGAAAC TGTACATAAA CCTGAATTGT CTTCAAAAAC TCCCACTTCC 
CCAAAAACTC TTATGAAAGC TCATAGTTCA ACACAGCCTC CAGAGTTTGA CAGTGGAGTT 
GAAACTTTCT CTGTTCACAC AGATAAGCCT AAATATCAAA TGAATAATAT CAGCACCATG 
CCTAAAGCTG TCCCTGTGAG TCCTTCAGCT GTGGAAGAAG ATGAAGATGA GGATGGTCAT 
ACTGTAGTGG CTACAGCTCG TGGCATTTTT AACAGCAATG GTGGTGTGTT GAGTTCCATA 
GAAACCGGTG TTAGTATAAT TATCCCACAA GGAGCCATTC CTGAAGGAAT TGAGCAAGAA 
ATCTATTTCA AAGTCTGCAG AGACAATAGC ATTCTCCCAC CTTTAGATAA AGAGAAAGGT 
GAAACTCTGC TGAGCCCCCT AGTGATGTGT GGGCCCCATG GCCTCAAGTT CCTGAAGCCC 
GTGGAGCTAC GCTTGCCACA CTGTGACCCT AAAACCTGGC AAAACAAGTG TCTTCCTGGA 
GATCCGAATT ACCTTGTTGG AGCCAACTGT GTTTCTGTCC TGATTGACCA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006540849.1
CDS	72..5348
Translation	MRRLEGLCLRRRRRRRGPARERSGDKMKYQKYLTVLQMAIGVTPSNRGSLLPLKRKLWVTPSSENPHGATSTVSQGKPSLRRIKGRLHRSKSLDSIDFCELTSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSHPDPEPVSDNEDDSYDEEVHDPRAGRGALANRRSEKSWARDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSQSRSPDQRSEPSDHSTQSPQQPSNGSLRSREEERMSKPGAISTPVKHVDDHPPKAVEEVTVEKNEKQTPTLPEPKPVYAQVGQPDVDLPVSPSDGALPNSAHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDHRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQAIHRIDSPGLKPASQQVYRKEPYSEEMMRQNHILKQPALGHPGQRPDKEPNLAYEPQLPYIEKQASRDLEQPSYRYEVSSYTDQFSRNYDHRLRFEDRIPTYEDQWSYYDDKQPYQPRPFENQHPRDLDSRQHPEEASERGYFQRFEEPAPLSYDSRTRYEQLPRTSTLRHEEQPAPAYEVHNRYRPEAQPYSSTGPKSSEPKQYFDQYPRSYEQVPPPGFTSKTGHYEPLHGAAVVPPLIPSSQQKPEVLPSATKPQPPPPTLTEEEEDPAMKPQSVLTRVKMFENKRSASLENKKDVNDTASFKPPEVASKPPGASLAGPKPVPQSQFSEHDKTLYRLPEPQKPQVKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFESKPSAHLPAGHHSEPAKPVHSQSQPNFSSYSSKGKPETDAVDRSFSEKRYDPAQATPPPPPLPSQYSQPAPPLSSSSLHIHSKGAQGEGNSVSLDFQNSYMSKPDPPPSQSKPATFRPPTREDPPQTFYPQKSFPDKAPVNGAEQTQKTITPVYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETVHKPELSSKTPTSPKTLMKAHSSTQPPEFDSGVETFSVHTDKPKYQMNNISTMPKAVPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_006540786.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus tight junction protein 1 (Tjp1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006540786.3

ORF Insert Sequence:

ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT 
CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA 
CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC 
AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA 
CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA 
AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT 
GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG 
AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG 
TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC 
ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT 
GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT 
GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA 
CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC 
TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT 
GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA 
CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG 
CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT 
AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG 
GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT 
GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT 
CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC 
CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT 
CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT 
GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT 
ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG 
TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA 
ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA 
ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT 
ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC 
ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT 
GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC 
CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT 
CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT 
CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA 
GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT 
AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG 
ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA 
ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT 
CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGGTGTACAG GAAGGAGCCG 
TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA 
GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA 
AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA 
GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT 
GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT 
CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT 
TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG 
CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC 
AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC 
AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC 
TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT 
TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA 
ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC 
AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT 
GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT 
AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG 
CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT 
GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG 
AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT 
TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAGG GAAAACCCGA AACTGATGCT 
GTGGATAGAT CATTCAGTGA GAAACGTTAT GATCCAGCCC AGGCCACGCC TCCTCCTCCT 
CCGTTGCCCT CACAGTACAG CCAGCCAGCT CCACCTCTGT CCAGCTCTTC TCTCCACATA 
CATTCCAAGG GCGCCCAGGG TGAAGGCAAC TCAGTATCAT TGGATTTTCA GAACTCATAT 
ATGTCCAAAC CAGACCCACC CCCATCTCAG AGTAAACCAG CAACTTTCAG ACCACCAACT 
CGAGAAGACC CCCCTCAGAC TTTCTATCCG CAGAAAAGTT TCCCAGACAA AGCTCCAGTT 
AACGGAGCTG AGCAGACTCA GAAAACCATC ACTCCGGTGT ACAATCGATT CACACCAAAG 
CCGTACACAA GTTCTGCCCG GCCATTTGAA CGCAAATTTG AAAGTCCGAA GTTCAACCAT 
AATCTTCTGC CAAGTGAAAC TGTACATAAA CCTGAATTGT CTTCAAAAAC TCCCACTTCC 
CCAAAAACTC TTATGAAAGC TCATAGTTCA ACACAGCCTC CAGAGTTTGA CAGTGGAGTT 
GAAACTTTCT CTGTTCACAC AGATAAGCCT AAATATCAAA TGAATAATAT CAGCACCATG 
CCTAAAGCTG TCCCTGTGAG TCCTTCAGCT GTGGAAGAAG ATGAAGATGA GGATGGTCAT 
ACTGTAGTGG CTACAGCTCG TGGCATTTTT AACAGCAATG GTGGTGTGTT GAGTTCCATA 
GAAACCGGTG TTAGTATAAT TATCCCACAA GGAGCCATTC CTGAAGGAAT TGAGCAAGAA 
ATCTATTTCA AAGTCTGCAG AGACAATAGC ATTCTCCCAC CTTTAGATAA AGAGAAAGGT 
GAAACTCTGC TGAGCCCCCT AGTGATGTGT GGGCCCCATG GCCTCAAGTT CCTGAAGCCC 
GTGGAGCTAC GCTTGCCACA CTGTGACCCT AAAACCTGGC AAAACAAGTG TCTTCCTGGA 
GATCCGAATT ACCTTGTTGG AGCCAACTGT GTTTCTGTCC TGATTGACCA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tjp1 ( XM_011250843.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011249145.1 Mus musculus tight junction protein 1 (Tjp1), transcript variant X5, mRNA.

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CloneID	OMu68895
Clone ID Related Accession (Same CDS sequence)	XM_011250843.2 , XM_011250843.3
Accession Version	XM_011250843.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5538bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	tight junction protein ZO-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011250843.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT 
CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA 
CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC 
AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA 
CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA 
AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT 
GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG 
AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG 
TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC 
ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT 
GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT 
GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA 
CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC 
TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT 
GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA 
CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG 
CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT 
AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG 
GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT 
GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT 
CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC 
CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT 
CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT 
GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT 
ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG 
TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA 
ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA 
ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT 
ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC 
ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT 
GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC 
CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT 
CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT 
CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA 
GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT 
AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG 
ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA 
ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT 
CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCCTCATCT 
CCAGTCCCTT ACCTTTCGCC TGAAACAACC CCAGCATCAT CAGCCTCTGC AGTTAATCAT 
AATGTCAGTG TAACTAATGT CAGCCTGGAG GAGCCTGCCC CAGCCCCTCC CACCTCGCAC 
GCATCACAGC CTGGTTGTTT AGGAGCACCA AGTGCTGAGG CAGCTCACGT AGTGCTCAGA 
GGTGAAGGAC CACCATTGCC GCCGCATGCA GACCCAGCAA AGGTGTACAG GAAGGAGCCG 
TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA 
GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA 
AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA 
GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT 
GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT 
CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT 
TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG 
CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC 
AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC 
AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC 
TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT 
TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA 
ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC 
AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT 
GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT 
AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG 
CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT 
GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG 
AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT 
TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAGG GAAAACCCGA AACTGATGCT 
GTGGATAGAT CATTCAGTGA GAAACGTTAT GATCCAGCCC AGGCCACGCC TCCTCCTCCT 
CCGTTGCCCT CACAGTACAG CCAGCCAGCT CCACCTCTGT CCAGCTCTTC TCTCCACATA 
CATTCCAAGG GCGCCCAGGG TGAAGGCAAC TCAGTATCAT TGGATTTTCA GAACTCATAT 
ATGTCCAAAC CAGACCCACC CCCATCTCAG AGTAAACCAG CAACTTTCAG ACCACCAACT 
CGAGAAGACC CCCCTCAGAC TTTCTATCCG CAGAAAAGTT TCCCAGACAA AGCTCCAGTT 
AACGGAGCTG AGCAGACTCA GAAAACCATC ACTCCGGTGT ACAATCGATT CACACCAAAG 
CCGTACACAA GTTCTGCCCG GCCATTTGAA CGCAAATTTG AAAGTCCGAA GTTCAACCAT 
AATCTTCTGC CAAGTGAAAC TGTACATAAA CCTGAATTGT CTTCAAAAAC TCCCACTTCC 
CCAAAAACTC TTATGAAAGC TCATAGTTCA ACACAGCCTC CAGAGTTTGA CAGTGGAGTT 
GAAACTTTCT CTGTTCACAC AGATAAGCCT AAATATCAAA TGAATAATAT CAGCACCATG 
CCTAAAGCTG TCCCTGTGAG TCCTTCAGCT GTGGAAGAAG ATGAAGATGA GGATGGTCAT 
ACTGTAGTGG CTACAGCTCG TGGCATTTTT AACAGCAATG GTGGTGTGTT GAGTTCCATA 
GAAACCGGTG TTAGTATAAT TATCCCACAA GGAGCCATTC CTGAAGGAAT TGAGCAAGAA 
ATCTATTTCA AAGTCTGCAG AGACAATAGC ATTCTCCCAC CTTTAGATAA AGAGAAAGGT 
GAAACTCTGC TGAGCCCCCT AGTGATGTGT GGGCCCCATG GCCTCAAGTT CCTGAAGCCC 
GTGGAGCTAC GCTTGCCACA CTGTGCGTCC ATGACTCCTG ACGGTGACCC TAAAACCTGG 
CAAAACAAGT GTCTTCCTGG AGATCCGAAT TACCTTGTTG GAGCCAACTG TGTTTCTGTC 
CTGATTGACC ACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011249145.1
CDS	72..5609
Translation	MRRLEGLCLRRRRRRRGPARERSGDKMKYQKYLTVLQMAIGVTPSNRGSLLPLKRKLWVTPSSENPHGATSTVSQGKPSLRRIKGRLHRSKSLDSIDFCELTSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSHPDPEPVSDNEDDSYDEEVHDPRAGRGALANRRSEKSWARDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSQSRSPDQRSEPSDHSTQSPQQPSNGSLRSREEERMSKPGAISTPVKHVDDHPPKAVEEVTVEKNEKQTPTLPEPKPVYAQVGQPDVDLPVSPSDGALPNSAHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDHRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQAIHRIDSPGLKPASQQKAEASSPVPYLSPETTPASSASAVNHNVSVTNVSLEEPAPAPPTSHASQPGCLGAPSAEAAHVVLRGEGPPLPPHADPAKVYRKEPYSEEMMRQNHILKQPALGHPGQRPDKEPNLAYEPQLPYIEKQASRDLEQPSYRYEVSSYTDQFSRNYDHRLRFEDRIPTYEDQWSYYDDKQPYQPRPFENQHPRDLDSRQHPEEASERGYFQRFEEPAPLSYDSRTRYEQLPRTSTLRHEEQPAPAYEVHNRYRPEAQPYSSTGPKSSEPKQYFDQYPRSYEQVPPPGFTSKTGHYEPLHGAAVVPPLIPSSQQKPEVLPSATKPQPPPPTLTEEEEDPAMKPQSVLTRVKMFENKRSASLENKKDVNDTASFKPPEVASKPPGASLAGPKPVPQSQFSEHDKTLYRLPEPQKPQVKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFESKPSAHLPAGHHSEPAKPVHSQSQPNFSSYSSKGKPETDAVDRSFSEKRYDPAQATPPPPPLPSQYSQPAPPLSSSSLHIHSKGAQGEGNSVSLDFQNSYMSKPDPPPSQSKPATFRPPTREDPPQTFYPQKSFPDKAPVNGAEQTQKTITPVYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETVHKPELSSKTPTSPKTLMKAHSSTQPPEFDSGVETFSVHTDKPKYQMNNISTMPKAVPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_011250843.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus tight junction protein 1 (Tjp1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011250843.2

ORF Insert Sequence:

ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT 
CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA 
CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC 
AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA 
CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA 
AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT 
GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG 
AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG 
TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC 
ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT 
GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT 
GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA 
CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC 
TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT 
GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA 
CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG 
CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT 
AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG 
GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT 
GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT 
CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC 
CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT 
CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT 
GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT 
ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG 
TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA 
ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA 
ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT 
ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC 
ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT 
GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC 
CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT 
CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT 
CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA 
GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT 
AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG 
ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA 
ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT 
CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCCTCATCT 
CCAGTCCCTT ACCTTTCGCC TGAAACAACC CCAGCATCAT CAGCCTCTGC AGTTAATCAT 
AATGTCAGTG TAACTAATGT CAGCCTGGAG GAGCCTGCCC CAGCCCCTCC CACCTCGCAC 
GCATCACAGC CTGGTTGTTT AGGAGCACCA AGTGCTGAGG CAGCTCACGT AGTGCTCAGA 
GGTGAAGGAC CACCATTGCC GCCGCATGCA GACCCAGCAA AGGTGTACAG GAAGGAGCCG 
TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA 
GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA 
AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA 
GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT 
GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT 
CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT 
TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG 
CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC 
AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC 
AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC 
TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT 
TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA 
ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC 
AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT 
GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT 
AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG 
CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT 
GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG 
AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT 
TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAGG GAAAACCCGA AACTGATGCT 
GTGGATAGAT CATTCAGTGA GAAACGTTAT GATCCAGCCC AGGCCACGCC TCCTCCTCCT 
CCGTTGCCCT CACAGTACAG CCAGCCAGCT CCACCTCTGT CCAGCTCTTC TCTCCACATA 
CATTCCAAGG GCGCCCAGGG TGAAGGCAAC TCAGTATCAT TGGATTTTCA GAACTCATAT 
ATGTCCAAAC CAGACCCACC CCCATCTCAG AGTAAACCAG CAACTTTCAG ACCACCAACT 
CGAGAAGACC CCCCTCAGAC TTTCTATCCG CAGAAAAGTT TCCCAGACAA AGCTCCAGTT 
AACGGAGCTG AGCAGACTCA GAAAACCATC ACTCCGGTGT ACAATCGATT CACACCAAAG 
CCGTACACAA GTTCTGCCCG GCCATTTGAA CGCAAATTTG AAAGTCCGAA GTTCAACCAT 
AATCTTCTGC CAAGTGAAAC TGTACATAAA CCTGAATTGT CTTCAAAAAC TCCCACTTCC 
CCAAAAACTC TTATGAAAGC TCATAGTTCA ACACAGCCTC CAGAGTTTGA CAGTGGAGTT 
GAAACTTTCT CTGTTCACAC AGATAAGCCT AAATATCAAA TGAATAATAT CAGCACCATG 
CCTAAAGCTG TCCCTGTGAG TCCTTCAGCT GTGGAAGAAG ATGAAGATGA GGATGGTCAT 
ACTGTAGTGG CTACAGCTCG TGGCATTTTT AACAGCAATG GTGGTGTGTT GAGTTCCATA 
GAAACCGGTG TTAGTATAAT TATCCCACAA GGAGCCATTC CTGAAGGAAT TGAGCAAGAA 
ATCTATTTCA AAGTCTGCAG AGACAATAGC ATTCTCCCAC CTTTAGATAA AGAGAAAGGT 
GAAACTCTGC TGAGCCCCCT AGTGATGTGT GGGCCCCATG GCCTCAAGTT CCTGAAGCCC 
GTGGAGCTAC GCTTGCCACA CTGTGCGTCC ATGACTCCTG ACGGTGACCC TAAAACCTGG 
CAAAACAAGT GTCTTCCTGG AGATCCGAAT TACCTTGTTG GAGCCAACTG TGTTTCTGTC 
CTGATTGACC ACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Tjp1
Entrez Gene ID	21872
Full Name	tight junction protein 1
Synonyms	ZO1
General protein information	Preferred Names tight junction protein 1 Names tight junction protein ZO-1 zona occludens protein 1 zonula occludens protein 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7 C\|7 35.02 cM

Homo sapiens (human)	TJP1	NP_003248.3
	TJP1	XP_002804738.1
Canis lupus familiaris (dog)	TJP1	NP_001003140.1
Rattus norvegicus (Norway rat)	Tjp1	NP_001099736.1
Danio rerio (zebrafish)	LOC565459	XP_003201509.2
Bos taurus (cattle)	TJP1	XP_002696696.3
Gallus gallus (chicken)	TJP1	XP_413773.4
Danio rerio (zebrafish)	tjp1a	XP_005168990.1
Pan troglodytes (chimpanzee)	TJP1	XP_001163120.1
Mus musculus (house mouse)	Tjp1	NP_033412.2
Xenopus tropicalis (tropical clawed frog)	tjp1	XP_004917990.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Tjp1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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