Tjp1 cDNA ORF clone, Mus musculus(house mouse)

The following Tjp1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tjp1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu38768 XM_006540778.4
Latest version!
Mus musculus tight junction protein 1 (Tjp1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu38769 XM_006540779.4
Latest version!
Mus musculus tight junction protein 1 (Tjp1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu38770 XM_006540780.4
Latest version!
Mus musculus tight junction protein 1 (Tjp1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu38771 XM_006540781.4
Latest version!
Mus musculus tight junction protein 1 (Tjp1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu38772 XM_006540782.4
Latest version!
Mus musculus tight junction protein 1 (Tjp1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu38773 XM_006540783.4
Latest version!
Mus musculus tight junction protein 1 (Tjp1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu38774 XM_006540784.4
Latest version!
Mus musculus tight junction protein 1 (Tjp1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu38775 XM_006540785.4
Latest version!
Mus musculus tight junction protein 1 (Tjp1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu38776 XM_006540786.4
Latest version!
Mus musculus tight junction protein 1 (Tjp1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu68895 XM_011250843.3
Latest version!
Mus musculus tight junction protein 1 (Tjp1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu89424 XM_030242356.1
Latest version!
Mus musculus tight junction protein 1 (Tjp1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu20010 NM_009386.2
Latest version!
Mus musculus tight junction protein 1 (Tjp1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1280.30
$1829.00
OMu22535 NM_001163574.1
Latest version!
Mus musculus tight junction protein 1 (Tjp1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
View Old Accession Versions >>
OMu38771 XM_006540781.3 Mus musculus tight junction protein 1 (Tjp1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
OMu38776 XM_006540786.3 Mus musculus tight junction protein 1 (Tjp1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
OMu38769 XM_006540779.3 Mus musculus tight junction protein 1 (Tjp1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
OMu38773 XM_006540783.3 Mus musculus tight junction protein 1 (Tjp1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
OMu38768 XM_006540778.3 Mus musculus tight junction protein 1 (Tjp1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
OMu68895 XM_011250843.2 Mus musculus tight junction protein 1 (Tjp1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
OMu38770 XM_006540780.3 Mus musculus tight junction protein 1 (Tjp1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
OMu38772 XM_006540782.3 Mus musculus tight junction protein 1 (Tjp1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
OMu38774 XM_006540784.3 Mus musculus tight junction protein 1 (Tjp1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OMu38775 XM_006540785.3 Mus musculus tight junction protein 1 (Tjp1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu38768
    Clone ID Related Accession (Same CDS sequence) XM_006540778.3 , XM_006540778.4
    Accession Version XM_006540778.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5628bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product tight junction protein ZO-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006540778.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT
    CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA
    CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC
    AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA
    CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA
    AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT
    GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG
    AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG
    TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC
    ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT
    GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT
    GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA
    CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC
    TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT
    GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA
    CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG
    CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT
    AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG
    GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT
    GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT
    CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC
    CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT
    CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT
    GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT
    ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG
    TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA
    ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA
    ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT
    ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC
    ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT
    GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC
    CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT
    CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT
    CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA
    GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT
    AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG
    ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA
    ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT
    CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCCTCATCT
    CCAGTCCCTT ACCTTTCGCC TGAAACAACC CCAGCATCAT CAGCCTCTGC AGTTAATCAT
    AATGTCAGTG TAACTAATGT CAGCCTGGAG GAGCCTGCCC CAGCCCCTCC CACCTCGCAC
    GCATCACAGC CTGGTTGTTT AGGAGCACCA AGTGCTGAGG CAGCTCACGT AGTGCTCAGA
    GGTGAAGGAC CACCATTGCC GCCGCATGCA GACCCAGCAA AGGTGTACAG GAAGGAGCCG
    TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA
    GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA
    AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA
    GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT
    GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT
    CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT
    TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG
    CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC
    AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC
    AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC
    TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT
    TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA
    ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC
    AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT
    GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT
    AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG
    CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT
    GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG
    AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT
    TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAAT TTCTTGGCTC TTACACTAGC
    TATGACTACT TGAAAAGGAA CATCAAGTGG GGAAAACCCG AAACTGATGC TGTGGATAGA
    TCATTCAGTG AGAAACGTTA TGATCCAGCC CAGGCCACGC CTCCTCCTCC TCCGTTGCCC
    TCACAGTACA GCCAGCCAGC TCCACCTCTG TCCAGCTCTT CTCTCCACAT ACATTCCAAG
    GGCGCCCAGG GTGAAGGCAA CTCAGTATCA TTGGATTTTC AGAACTCATA TATGTCCAAA
    CCAGACCCAC CCCCATCTCA GAGTAAACCA GCAACTTTCA GACCACCAAC TCGAGAAGAC
    CCCCCTCAGA CTTTCTATCC GCAGAAAAGT TTCCCAGACA AAGCTCCAGT TAACGGAGCT
    GAGCAGACTC AGAAAACCAT CACTCCGGTG TACAATCGAT TCACACCAAA GCCGTACACA
    AGTTCTGCCC GGCCATTTGA ACGCAAATTT GAAAGTCCGA AGTTCAACCA TAATCTTCTG
    CCAAGTGAAA CTGTACATAA ACCTGAATTG TCTTCAAAAA CTCCCACTTC CCCAAAAACT
    CTTATGAAAG CTCATAGTTC AACACAGCCT CCAGAGTTTG ACAGTGGAGT TGAAACTTTC
    TCTGTTCACA CAGATAAGCC TAAATATCAA ATGAATAATA TCAGCACCAT GCCTAAAGCT
    GTCCCTGTGA GTCCTTCAGC TGTGGAAGAA GATGAAGATG AGGATGGTCA TACTGTAGTG
    GCTACAGCTC GTGGCATTTT TAACAGCAAT GGTGGTGTGT TGAGTTCCAT AGAAACCGGT
    GTTAGTATAA TTATCCCACA AGGAGCCATT CCTGAAGGAA TTGAGCAAGA AATCTATTTC
    AAAGTCTGCA GAGACAATAG CATTCTCCCA CCTTTAGATA AAGAGAAAGG TGAAACTCTG
    CTGAGCCCCC TAGTGATGTG TGGGCCCCAT GGCCTCAAGT TCCTGAAGCC CGTGGAGCTA
    CGCTTGCCAC ACTGTGCGTC CATGACTCCT GACGGTTGGT CTTTTGCTCT AAAATCATCC
    GACTCCTCGT CGGGTGACCC TAAAACCTGG CAAAACAAGT GTCTTCCTGG AGATCCGAAT
    TACCTTGTTG GAGCCAACTG TGTTTCTGTC CTGATTGACC ACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006540841.1
    CDS115..5742
    Translation

    Target ORF information:

    RefSeq Version XM_006540778.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus tight junction protein 1 (Tjp1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006540778.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT
    CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA
    CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC
    AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA
    CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA
    AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT
    GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG
    AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG
    TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC
    ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT
    GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT
    GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA
    CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC
    TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT
    GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA
    CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG
    CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT
    AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG
    GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT
    GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT
    CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC
    CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT
    CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT
    GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT
    ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG
    TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA
    ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA
    ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT
    ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC
    ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT
    GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC
    CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT
    CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT
    CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA
    GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT
    AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG
    ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA
    ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT
    CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCCTCATCT
    CCAGTCCCTT ACCTTTCGCC TGAAACAACC CCAGCATCAT CAGCCTCTGC AGTTAATCAT
    AATGTCAGTG TAACTAATGT CAGCCTGGAG GAGCCTGCCC CAGCCCCTCC CACCTCGCAC
    GCATCACAGC CTGGTTGTTT AGGAGCACCA AGTGCTGAGG CAGCTCACGT AGTGCTCAGA
    GGTGAAGGAC CACCATTGCC GCCGCATGCA GACCCAGCAA AGGTGTACAG GAAGGAGCCG
    TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA
    GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA
    AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA
    GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT
    GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT
    CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT
    TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG
    CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC
    AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC
    AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC
    TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT
    TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA
    ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC
    AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT
    GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT
    AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG
    CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT
    GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG
    AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT
    TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAAT TTCTTGGCTC TTACACTAGC
    TATGACTACT TGAAAAGGAA CATCAAGTGG GGAAAACCCG AAACTGATGC TGTGGATAGA
    TCATTCAGTG AGAAACGTTA TGATCCAGCC CAGGCCACGC CTCCTCCTCC TCCGTTGCCC
    TCACAGTACA GCCAGCCAGC TCCACCTCTG TCCAGCTCTT CTCTCCACAT ACATTCCAAG
    GGCGCCCAGG GTGAAGGCAA CTCAGTATCA TTGGATTTTC AGAACTCATA TATGTCCAAA
    CCAGACCCAC CCCCATCTCA GAGTAAACCA GCAACTTTCA GACCACCAAC TCGAGAAGAC
    CCCCCTCAGA CTTTCTATCC GCAGAAAAGT TTCCCAGACA AAGCTCCAGT TAACGGAGCT
    GAGCAGACTC AGAAAACCAT CACTCCGGTG TACAATCGAT TCACACCAAA GCCGTACACA
    AGTTCTGCCC GGCCATTTGA ACGCAAATTT GAAAGTCCGA AGTTCAACCA TAATCTTCTG
    CCAAGTGAAA CTGTACATAA ACCTGAATTG TCTTCAAAAA CTCCCACTTC CCCAAAAACT
    CTTATGAAAG CTCATAGTTC AACACAGCCT CCAGAGTTTG ACAGTGGAGT TGAAACTTTC
    TCTGTTCACA CAGATAAGCC TAAATATCAA ATGAATAATA TCAGCACCAT GCCTAAAGCT
    GTCCCTGTGA GTCCTTCAGC TGTGGAAGAA GATGAAGATG AGGATGGTCA TACTGTAGTG
    GCTACAGCTC GTGGCATTTT TAACAGCAAT GGTGGTGTGT TGAGTTCCAT AGAAACCGGT
    GTTAGTATAA TTATCCCACA AGGAGCCATT CCTGAAGGAA TTGAGCAAGA AATCTATTTC
    AAAGTCTGCA GAGACAATAG CATTCTCCCA CCTTTAGATA AAGAGAAAGG TGAAACTCTG
    CTGAGCCCCC TAGTGATGTG TGGGCCCCAT GGCCTCAAGT TCCTGAAGCC CGTGGAGCTA
    CGCTTGCCAC ACTGTGCGTC CATGACTCCT GACGGTTGGT CTTTTGCTCT AAAATCATCC
    GACTCCTCGT CGGGTGACCC TAAAACCTGG CAAAACAAGT GTCTTCCTGG AGATCCGAAT
    TACCTTGTTG GAGCCAACTG TGTTTCTGTC CTGATTGACC ACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu38769
    Clone ID Related Accession (Same CDS sequence) XM_006540779.3 , XM_006540779.4
    Accession Version XM_006540779.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5589bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product tight junction protein ZO-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006540779.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT
    CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA
    CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC
    AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA
    CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA
    AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT
    GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG
    AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG
    TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC
    ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT
    GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT
    GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA
    CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC
    TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT
    GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA
    CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG
    CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT
    AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG
    GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT
    GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT
    CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC
    CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT
    CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT
    GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT
    ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG
    TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA
    ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA
    ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT
    ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC
    ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT
    GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC
    CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT
    CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT
    CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA
    GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT
    AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG
    ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA
    ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT
    CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCCTCATCT
    CCAGTCCCTT ACCTTTCGCC TGAAACAACC CCAGCATCAT CAGCCTCTGC AGTTAATCAT
    AATGTCAGTG TAACTAATGT CAGCCTGGAG GAGCCTGCCC CAGCCCCTCC CACCTCGCAC
    GCATCACAGC CTGGTTGTTT AGGAGCACCA AGTGCTGAGG CAGCTCACGT AGTGCTCAGA
    GGTGAAGGAC CACCATTGCC GCCGCATGCA GACCCAGCAA AGGTGTACAG GAAGGAGCCG
    TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA
    GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA
    AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA
    GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT
    GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT
    CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT
    TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG
    CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC
    AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC
    AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC
    TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT
    TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA
    ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC
    AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT
    GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT
    AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG
    CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT
    GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG
    AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT
    TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAAT TTCTTGGCTC TTACACTAGC
    TATGACTACT TGAAAAGGAA CATCAAGTGG GGAAAACCCG AAACTGATGC TGTGGATAGA
    TCATTCAGTG AGAAACGTTA TGATCCAGCC CAGGCCACGC CTCCTCCTCC TCCGTTGCCC
    TCACAGTACA GCCAGCCAGC TCCACCTCTG TCCAGCTCTT CTCTCCACAT ACATTCCAAG
    GGCGCCCAGG GTGAAGGCAA CTCAGTATCA TTGGATTTTC AGAACTCATA TATGTCCAAA
    CCAGACCCAC CCCCATCTCA GAGTAAACCA GCAACTTTCA GACCACCAAC TCGAGAAGAC
    CCCCCTCAGA CTTTCTATCC GCAGAAAAGT TTCCCAGACA AAGCTCCAGT TAACGGAGCT
    GAGCAGACTC AGAAAACCAT CACTCCGGTG TACAATCGAT TCACACCAAA GCCGTACACA
    AGTTCTGCCC GGCCATTTGA ACGCAAATTT GAAAGTCCGA AGTTCAACCA TAATCTTCTG
    CCAAGTGAAA CTGTACATAA ACCTGAATTG TCTTCAAAAA CTCCCACTTC CCCAAAAACT
    CTTATGAAAG CTCATAGTTC AACACAGCCT CCAGAGTTTG ACAGTGGAGT TGAAACTTTC
    TCTGTTCACA CAGATAAGCC TAAATATCAA ATGAATAATA TCAGCACCAT GCCTAAAGCT
    GTCCCTGTGA GTCCTTCAGC TGTGGAAGAA GATGAAGATG AGGATGGTCA TACTGTAGTG
    GCTACAGCTC GTGGCATTTT TAACAGCAAT GGTGGTGTGT TGAGTTCCAT AGAAACCGGT
    GTTAGTATAA TTATCCCACA AGGAGCCATT CCTGAAGGAA TTGAGCAAGA AATCTATTTC
    AAAGTCTGCA GAGACAATAG CATTCTCCCA CCTTTAGATA AAGAGAAAGG TGAAACTCTG
    CTGAGCCCCC TAGTGATGTG TGGGCCCCAT GGCCTCAAGT TCCTGAAGCC CGTGGAGCTA
    CGCTTGCCAC ACTGTGCGTC CATGACTCCT GACGGTGACC CTAAAACCTG GCAAAACAAG
    TGTCTTCCTG GAGATCCGAA TTACCTTGTT GGAGCCAACT GTGTTTCTGT CCTGATTGAC
    CACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006540842.1
    CDS115..5703
    Translation

    Target ORF information:

    RefSeq Version XM_006540779.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus tight junction protein 1 (Tjp1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006540779.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT
    CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA
    CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC
    AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA
    CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA
    AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT
    GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG
    AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG
    TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC
    ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT
    GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT
    GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA
    CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC
    TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT
    GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA
    CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG
    CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT
    AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG
    GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT
    GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT
    CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC
    CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT
    CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT
    GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT
    ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG
    TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA
    ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA
    ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT
    ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC
    ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT
    GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC
    CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT
    CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT
    CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA
    GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT
    AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG
    ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA
    ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT
    CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCCTCATCT
    CCAGTCCCTT ACCTTTCGCC TGAAACAACC CCAGCATCAT CAGCCTCTGC AGTTAATCAT
    AATGTCAGTG TAACTAATGT CAGCCTGGAG GAGCCTGCCC CAGCCCCTCC CACCTCGCAC
    GCATCACAGC CTGGTTGTTT AGGAGCACCA AGTGCTGAGG CAGCTCACGT AGTGCTCAGA
    GGTGAAGGAC CACCATTGCC GCCGCATGCA GACCCAGCAA AGGTGTACAG GAAGGAGCCG
    TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA
    GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA
    AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA
    GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT
    GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT
    CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT
    TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG
    CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC
    AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC
    AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC
    TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT
    TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA
    ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC
    AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT
    GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT
    AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG
    CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT
    GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG
    AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT
    TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAAT TTCTTGGCTC TTACACTAGC
    TATGACTACT TGAAAAGGAA CATCAAGTGG GGAAAACCCG AAACTGATGC TGTGGATAGA
    TCATTCAGTG AGAAACGTTA TGATCCAGCC CAGGCCACGC CTCCTCCTCC TCCGTTGCCC
    TCACAGTACA GCCAGCCAGC TCCACCTCTG TCCAGCTCTT CTCTCCACAT ACATTCCAAG
    GGCGCCCAGG GTGAAGGCAA CTCAGTATCA TTGGATTTTC AGAACTCATA TATGTCCAAA
    CCAGACCCAC CCCCATCTCA GAGTAAACCA GCAACTTTCA GACCACCAAC TCGAGAAGAC
    CCCCCTCAGA CTTTCTATCC GCAGAAAAGT TTCCCAGACA AAGCTCCAGT TAACGGAGCT
    GAGCAGACTC AGAAAACCAT CACTCCGGTG TACAATCGAT TCACACCAAA GCCGTACACA
    AGTTCTGCCC GGCCATTTGA ACGCAAATTT GAAAGTCCGA AGTTCAACCA TAATCTTCTG
    CCAAGTGAAA CTGTACATAA ACCTGAATTG TCTTCAAAAA CTCCCACTTC CCCAAAAACT
    CTTATGAAAG CTCATAGTTC AACACAGCCT CCAGAGTTTG ACAGTGGAGT TGAAACTTTC
    TCTGTTCACA CAGATAAGCC TAAATATCAA ATGAATAATA TCAGCACCAT GCCTAAAGCT
    GTCCCTGTGA GTCCTTCAGC TGTGGAAGAA GATGAAGATG AGGATGGTCA TACTGTAGTG
    GCTACAGCTC GTGGCATTTT TAACAGCAAT GGTGGTGTGT TGAGTTCCAT AGAAACCGGT
    GTTAGTATAA TTATCCCACA AGGAGCCATT CCTGAAGGAA TTGAGCAAGA AATCTATTTC
    AAAGTCTGCA GAGACAATAG CATTCTCCCA CCTTTAGATA AAGAGAAAGG TGAAACTCTG
    CTGAGCCCCC TAGTGATGTG TGGGCCCCAT GGCCTCAAGT TCCTGAAGCC CGTGGAGCTA
    CGCTTGCCAC ACTGTGCGTC CATGACTCCT GACGGTGACC CTAAAACCTG GCAAAACAAG
    TGTCTTCCTG GAGATCCGAA TTACCTTGTT GGAGCCAACT GTGTTTCTGT CCTGATTGAC
    CACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu38770
    Clone ID Related Accession (Same CDS sequence) XM_006540780.4 , XM_006540780.3
    Accession Version XM_006540780.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5577bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product tight junction protein ZO-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006540780.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT
    CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA
    CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC
    AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA
    CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA
    AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT
    GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG
    AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG
    TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC
    ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT
    GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT
    GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA
    CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC
    TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT
    GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA
    CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG
    CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT
    AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG
    GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT
    GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT
    CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC
    CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT
    CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT
    GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT
    ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG
    TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA
    ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA
    ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT
    ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC
    ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT
    GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC
    CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT
    CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT
    CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA
    GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT
    AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG
    ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA
    ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT
    CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCCTCATCT
    CCAGTCCCTT ACCTTTCGCC TGAAACAACC CCAGCATCAT CAGCCTCTGC AGTTAATCAT
    AATGTCAGTG TAACTAATGT CAGCCTGGAG GAGCCTGCCC CAGCCCCTCC CACCTCGCAC
    GCATCACAGC CTGGTTGTTT AGGAGCACCA AGTGCTGAGG CAGCTCACGT AGTGCTCAGA
    GGTGAAGGAC CACCATTGCC GCCGCATGCA GACCCAGCAA AGGTGTACAG GAAGGAGCCG
    TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA
    GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA
    AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA
    GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT
    GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT
    CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT
    TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG
    CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC
    AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC
    AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC
    TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT
    TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA
    ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC
    AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT
    GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT
    AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG
    CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT
    GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG
    AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT
    TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAGG GAAAACCCGA AACTGATGCT
    GTGGATAGAT CATTCAGTGA GAAACGTTAT GATCCAGCCC AGGCCACGCC TCCTCCTCCT
    CCGTTGCCCT CACAGTACAG CCAGCCAGCT CCACCTCTGT CCAGCTCTTC TCTCCACATA
    CATTCCAAGG GCGCCCAGGG TGAAGGCAAC TCAGTATCAT TGGATTTTCA GAACTCATAT
    ATGTCCAAAC CAGACCCACC CCCATCTCAG AGTAAACCAG CAACTTTCAG ACCACCAACT
    CGAGAAGACC CCCCTCAGAC TTTCTATCCG CAGAAAAGTT TCCCAGACAA AGCTCCAGTT
    AACGGAGCTG AGCAGACTCA GAAAACCATC ACTCCGGTGT ACAATCGATT CACACCAAAG
    CCGTACACAA GTTCTGCCCG GCCATTTGAA CGCAAATTTG AAAGTCCGAA GTTCAACCAT
    AATCTTCTGC CAAGTGAAAC TGTACATAAA CCTGAATTGT CTTCAAAAAC TCCCACTTCC
    CCAAAAACTC TTATGAAAGC TCATAGTTCA ACACAGCCTC CAGAGTTTGA CAGTGGAGTT
    GAAACTTTCT CTGTTCACAC AGATAAGCCT AAATATCAAA TGAATAATAT CAGCACCATG
    CCTAAAGCTG TCCCTGTGAG TCCTTCAGCT GTGGAAGAAG ATGAAGATGA GGATGGTCAT
    ACTGTAGTGG CTACAGCTCG TGGCATTTTT AACAGCAATG GTGGTGTGTT GAGTTCCATA
    GAAACCGGTG TTAGTATAAT TATCCCACAA GGAGCCATTC CTGAAGGAAT TGAGCAAGAA
    ATCTATTTCA AAGTCTGCAG AGACAATAGC ATTCTCCCAC CTTTAGATAA AGAGAAAGGT
    GAAACTCTGC TGAGCCCCCT AGTGATGTGT GGGCCCCATG GCCTCAAGTT CCTGAAGCCC
    GTGGAGCTAC GCTTGCCACA CTGTGCGTCC ATGACTCCTG ACGGTTGGTC TTTTGCTCTA
    AAATCATCCG ACTCCTCGTC GGGTGACCCT AAAACCTGGC AAAACAAGTG TCTTCCTGGA
    GATCCGAATT ACCTTGTTGG AGCCAACTGT GTTTCTGTCC TGATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006540843.1
    CDS115..5691
    Translation

    Target ORF information:

    RefSeq Version XM_006540780.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus tight junction protein 1 (Tjp1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006540780.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT
    CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA
    CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC
    AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA
    CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA
    AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT
    GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG
    AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG
    TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC
    ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT
    GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT
    GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA
    CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC
    TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT
    GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA
    CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG
    CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT
    AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG
    GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT
    GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT
    CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC
    CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT
    CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT
    GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT
    ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG
    TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA
    ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA
    ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT
    ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC
    ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT
    GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC
    CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT
    CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT
    CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA
    GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT
    AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG
    ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA
    ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT
    CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCCTCATCT
    CCAGTCCCTT ACCTTTCGCC TGAAACAACC CCAGCATCAT CAGCCTCTGC AGTTAATCAT
    AATGTCAGTG TAACTAATGT CAGCCTGGAG GAGCCTGCCC CAGCCCCTCC CACCTCGCAC
    GCATCACAGC CTGGTTGTTT AGGAGCACCA AGTGCTGAGG CAGCTCACGT AGTGCTCAGA
    GGTGAAGGAC CACCATTGCC GCCGCATGCA GACCCAGCAA AGGTGTACAG GAAGGAGCCG
    TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA
    GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA
    AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA
    GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT
    GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT
    CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT
    TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG
    CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC
    AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC
    AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC
    TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT
    TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA
    ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC
    AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT
    GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT
    AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG
    CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT
    GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG
    AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT
    TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAGG GAAAACCCGA AACTGATGCT
    GTGGATAGAT CATTCAGTGA GAAACGTTAT GATCCAGCCC AGGCCACGCC TCCTCCTCCT
    CCGTTGCCCT CACAGTACAG CCAGCCAGCT CCACCTCTGT CCAGCTCTTC TCTCCACATA
    CATTCCAAGG GCGCCCAGGG TGAAGGCAAC TCAGTATCAT TGGATTTTCA GAACTCATAT
    ATGTCCAAAC CAGACCCACC CCCATCTCAG AGTAAACCAG CAACTTTCAG ACCACCAACT
    CGAGAAGACC CCCCTCAGAC TTTCTATCCG CAGAAAAGTT TCCCAGACAA AGCTCCAGTT
    AACGGAGCTG AGCAGACTCA GAAAACCATC ACTCCGGTGT ACAATCGATT CACACCAAAG
    CCGTACACAA GTTCTGCCCG GCCATTTGAA CGCAAATTTG AAAGTCCGAA GTTCAACCAT
    AATCTTCTGC CAAGTGAAAC TGTACATAAA CCTGAATTGT CTTCAAAAAC TCCCACTTCC
    CCAAAAACTC TTATGAAAGC TCATAGTTCA ACACAGCCTC CAGAGTTTGA CAGTGGAGTT
    GAAACTTTCT CTGTTCACAC AGATAAGCCT AAATATCAAA TGAATAATAT CAGCACCATG
    CCTAAAGCTG TCCCTGTGAG TCCTTCAGCT GTGGAAGAAG ATGAAGATGA GGATGGTCAT
    ACTGTAGTGG CTACAGCTCG TGGCATTTTT AACAGCAATG GTGGTGTGTT GAGTTCCATA
    GAAACCGGTG TTAGTATAAT TATCCCACAA GGAGCCATTC CTGAAGGAAT TGAGCAAGAA
    ATCTATTTCA AAGTCTGCAG AGACAATAGC ATTCTCCCAC CTTTAGATAA AGAGAAAGGT
    GAAACTCTGC TGAGCCCCCT AGTGATGTGT GGGCCCCATG GCCTCAAGTT CCTGAAGCCC
    GTGGAGCTAC GCTTGCCACA CTGTGCGTCC ATGACTCCTG ACGGTTGGTC TTTTGCTCTA
    AAATCATCCG ACTCCTCGTC GGGTGACCCT AAAACCTGGC AAAACAAGTG TCTTCCTGGA
    GATCCGAATT ACCTTGTTGG AGCCAACTGT GTTTCTGTCC TGATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu38771
    Clone ID Related Accession (Same CDS sequence) XM_006540781.3 , XM_006540781.4
    Accession Version XM_006540781.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5568bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product tight junction protein ZO-1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006540781.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT
    CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA
    CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC
    AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA
    CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA
    AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT
    GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG
    AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG
    TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC
    ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT
    GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT
    GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA
    CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC
    TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT
    GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA
    CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG
    CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT
    AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG
    GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT
    GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT
    CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC
    CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT
    CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT
    GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT
    ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG
    TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA
    ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA
    ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT
    ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC
    ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT
    GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC
    CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT
    CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT
    CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA
    GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT
    AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG
    ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA
    ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT
    CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCCTCATCT
    CCAGTCCCTT ACCTTTCGCC TGAAACAACC CCAGCATCAT CAGCCTCTGC AGTTAATCAT
    AATGTCAGTG TAACTAATGT CAGCCTGGAG GAGCCTGCCC CAGCCCCTCC CACCTCGCAC
    GCATCACAGC CTGGTTGTTT AGGAGCACCA AGTGCTGAGG CAGCTCACGT AGTGCTCAGA
    GGTGAAGGAC CACCATTGCC GCCGCATGCA GACCCAGCAA AGGTGTACAG GAAGGAGCCG
    TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA
    GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA
    AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA
    GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT
    GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT
    CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT
    TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG
    CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC
    AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC
    AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC
    TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT
    TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA
    ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC
    AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT
    GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT
    AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG
    CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT
    GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG
    AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT
    TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAAT TTCTTGGCTC TTACACTAGC
    TATGACTACT TGAAAAGGAA CATCAAGTGG GGAAAACCCG AAACTGATGC TGTGGATAGA
    TCATTCAGTG AGAAACGTTA TGATCCAGCC CAGGCCACGC CTCCTCCTCC TCCGTTGCCC
    TCACAGTACA GCCAGCCAGC TCCACCTCTG TCCAGCTCTT CTCTCCACAT ACATTCCAAG
    GGCGCCCAGG GTGAAGGCAA CTCAGTATCA TTGGATTTTC AGAACTCATA TATGTCCAAA
    CCAGACCCAC CCCCATCTCA GAGTAAACCA GCAACTTTCA GACCACCAAC TCGAGAAGAC
    CCCCCTCAGA CTTTCTATCC GCAGAAAAGT TTCCCAGACA AAGCTCCAGT TAACGGAGCT
    GAGCAGACTC AGAAAACCAT CACTCCGGTG TACAATCGAT TCACACCAAA GCCGTACACA
    AGTTCTGCCC GGCCATTTGA ACGCAAATTT GAAAGTCCGA AGTTCAACCA TAATCTTCTG
    CCAAGTGAAA CTGTACATAA ACCTGAATTG TCTTCAAAAA CTCCCACTTC CCCAAAAACT
    CTTATGAAAG CTCATAGTTC AACACAGCCT CCAGAGTTTG ACAGTGGAGT TGAAACTTTC
    TCTGTTCACA CAGATAAGCC TAAATATCAA ATGAATAATA TCAGCACCAT GCCTAAAGCT
    GTCCCTGTGA GTCCTTCAGC TGTGGAAGAA GATGAAGATG AGGATGGTCA TACTGTAGTG
    GCTACAGCTC GTGGCATTTT TAACAGCAAT GGTGGTGTGT TGAGTTCCAT AGAAACCGGT
    GTTAGTATAA TTATCCCACA AGGAGCCATT CCTGAAGGAA TTGAGCAAGA AATCTATTTC
    AAAGTCTGCA GAGACAATAG CATTCTCCCA CCTTTAGATA AAGAGAAAGG TGAAACTCTG
    CTGAGCCCCC TAGTGATGTG TGGGCCCCAT GGCCTCAAGT TCCTGAAGCC CGTGGAGCTA
    CGCTTGCCAC ACTGTGACCC TAAAACCTGG CAAAACAAGT GTCTTCCTGG AGATCCGAAT
    TACCTTGTTG GAGCCAACTG TGTTTCTGTC CTGATTGACC ACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006540844.1
    CDS115..5682
    Translation

    Target ORF information:

    RefSeq Version XM_006540781.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus tight junction protein 1 (Tjp1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006540781.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT
    CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA
    CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC
    AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA
    CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA
    AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT
    GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG
    AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG
    TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC
    ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT
    GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT
    GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA
    CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC
    TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT
    GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA
    CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG
    CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT
    AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG
    GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT
    GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT
    CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC
    CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT
    CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT
    GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT
    ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG
    TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA
    ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA
    ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT
    ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC
    ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT
    GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC
    CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT
    CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT
    CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA
    GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT
    AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG
    ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA
    ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT
    CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCCTCATCT
    CCAGTCCCTT ACCTTTCGCC TGAAACAACC CCAGCATCAT CAGCCTCTGC AGTTAATCAT
    AATGTCAGTG TAACTAATGT CAGCCTGGAG GAGCCTGCCC CAGCCCCTCC CACCTCGCAC
    GCATCACAGC CTGGTTGTTT AGGAGCACCA AGTGCTGAGG CAGCTCACGT AGTGCTCAGA
    GGTGAAGGAC CACCATTGCC GCCGCATGCA GACCCAGCAA AGGTGTACAG GAAGGAGCCG
    TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA
    GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA
    AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA
    GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT
    GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT
    CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT
    TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG
    CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC
    AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC
    AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC
    TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT
    TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA
    ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC
    AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT
    GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT
    AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG
    CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT
    GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG
    AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT
    TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAAT TTCTTGGCTC TTACACTAGC
    TATGACTACT TGAAAAGGAA CATCAAGTGG GGAAAACCCG AAACTGATGC TGTGGATAGA
    TCATTCAGTG AGAAACGTTA TGATCCAGCC CAGGCCACGC CTCCTCCTCC TCCGTTGCCC
    TCACAGTACA GCCAGCCAGC TCCACCTCTG TCCAGCTCTT CTCTCCACAT ACATTCCAAG
    GGCGCCCAGG GTGAAGGCAA CTCAGTATCA TTGGATTTTC AGAACTCATA TATGTCCAAA
    CCAGACCCAC CCCCATCTCA GAGTAAACCA GCAACTTTCA GACCACCAAC TCGAGAAGAC
    CCCCCTCAGA CTTTCTATCC GCAGAAAAGT TTCCCAGACA AAGCTCCAGT TAACGGAGCT
    GAGCAGACTC AGAAAACCAT CACTCCGGTG TACAATCGAT TCACACCAAA GCCGTACACA
    AGTTCTGCCC GGCCATTTGA ACGCAAATTT GAAAGTCCGA AGTTCAACCA TAATCTTCTG
    CCAAGTGAAA CTGTACATAA ACCTGAATTG TCTTCAAAAA CTCCCACTTC CCCAAAAACT
    CTTATGAAAG CTCATAGTTC AACACAGCCT CCAGAGTTTG ACAGTGGAGT TGAAACTTTC
    TCTGTTCACA CAGATAAGCC TAAATATCAA ATGAATAATA TCAGCACCAT GCCTAAAGCT
    GTCCCTGTGA GTCCTTCAGC TGTGGAAGAA GATGAAGATG AGGATGGTCA TACTGTAGTG
    GCTACAGCTC GTGGCATTTT TAACAGCAAT GGTGGTGTGT TGAGTTCCAT AGAAACCGGT
    GTTAGTATAA TTATCCCACA AGGAGCCATT CCTGAAGGAA TTGAGCAAGA AATCTATTTC
    AAAGTCTGCA GAGACAATAG CATTCTCCCA CCTTTAGATA AAGAGAAAGG TGAAACTCTG
    CTGAGCCCCC TAGTGATGTG TGGGCCCCAT GGCCTCAAGT TCCTGAAGCC CGTGGAGCTA
    CGCTTGCCAC ACTGTGACCC TAAAACCTGG CAAAACAAGT GTCTTCCTGG AGATCCGAAT
    TACCTTGTTG GAGCCAACTG TGTTTCTGTC CTGATTGACC ACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu38772
    Clone ID Related Accession (Same CDS sequence) XM_006540782.4 , XM_006540782.3
    Accession Version XM_006540782.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5517bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product tight junction protein ZO-1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006540782.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT
    CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA
    CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC
    AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA
    CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA
    AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT
    GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG
    AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG
    TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC
    ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT
    GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT
    GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA
    CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC
    TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT
    GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA
    CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG
    CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT
    AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG
    GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT
    GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT
    CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC
    CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT
    CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT
    GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT
    ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG
    TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA
    ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA
    ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT
    ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC
    ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT
    GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC
    CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT
    CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT
    CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA
    GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT
    AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG
    ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA
    ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT
    CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCCTCATCT
    CCAGTCCCTT ACCTTTCGCC TGAAACAACC CCAGCATCAT CAGCCTCTGC AGTTAATCAT
    AATGTCAGTG TAACTAATGT CAGCCTGGAG GAGCCTGCCC CAGCCCCTCC CACCTCGCAC
    GCATCACAGC CTGGTTGTTT AGGAGCACCA AGTGCTGAGG CAGCTCACGT AGTGCTCAGA
    GGTGAAGGAC CACCATTGCC GCCGCATGCA GACCCAGCAA AGGTGTACAG GAAGGAGCCG
    TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA
    GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA
    AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA
    GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT
    GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT
    CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT
    TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG
    CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC
    AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC
    AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC
    TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT
    TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA
    ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC
    AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT
    GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT
    AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG
    CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT
    GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG
    AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT
    TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAGG GAAAACCCGA AACTGATGCT
    GTGGATAGAT CATTCAGTGA GAAACGTTAT GATCCAGCCC AGGCCACGCC TCCTCCTCCT
    CCGTTGCCCT CACAGTACAG CCAGCCAGCT CCACCTCTGT CCAGCTCTTC TCTCCACATA
    CATTCCAAGG GCGCCCAGGG TGAAGGCAAC TCAGTATCAT TGGATTTTCA GAACTCATAT
    ATGTCCAAAC CAGACCCACC CCCATCTCAG AGTAAACCAG CAACTTTCAG ACCACCAACT
    CGAGAAGACC CCCCTCAGAC TTTCTATCCG CAGAAAAGTT TCCCAGACAA AGCTCCAGTT
    AACGGAGCTG AGCAGACTCA GAAAACCATC ACTCCGGTGT ACAATCGATT CACACCAAAG
    CCGTACACAA GTTCTGCCCG GCCATTTGAA CGCAAATTTG AAAGTCCGAA GTTCAACCAT
    AATCTTCTGC CAAGTGAAAC TGTACATAAA CCTGAATTGT CTTCAAAAAC TCCCACTTCC
    CCAAAAACTC TTATGAAAGC TCATAGTTCA ACACAGCCTC CAGAGTTTGA CAGTGGAGTT
    GAAACTTTCT CTGTTCACAC AGATAAGCCT AAATATCAAA TGAATAATAT CAGCACCATG
    CCTAAAGCTG TCCCTGTGAG TCCTTCAGCT GTGGAAGAAG ATGAAGATGA GGATGGTCAT
    ACTGTAGTGG CTACAGCTCG TGGCATTTTT AACAGCAATG GTGGTGTGTT GAGTTCCATA
    GAAACCGGTG TTAGTATAAT TATCCCACAA GGAGCCATTC CTGAAGGAAT TGAGCAAGAA
    ATCTATTTCA AAGTCTGCAG AGACAATAGC ATTCTCCCAC CTTTAGATAA AGAGAAAGGT
    GAAACTCTGC TGAGCCCCCT AGTGATGTGT GGGCCCCATG GCCTCAAGTT CCTGAAGCCC
    GTGGAGCTAC GCTTGCCACA CTGTGACCCT AAAACCTGGC AAAACAAGTG TCTTCCTGGA
    GATCCGAATT ACCTTGTTGG AGCCAACTGT GTTTCTGTCC TGATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006540845.1
    CDS115..5631
    Translation

    Target ORF information:

    RefSeq Version XM_006540782.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus tight junction protein 1 (Tjp1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006540782.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT
    CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA
    CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC
    AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA
    CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA
    AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT
    GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG
    AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG
    TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC
    ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT
    GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT
    GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA
    CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC
    TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT
    GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA
    CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG
    CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT
    AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG
    GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT
    GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT
    CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC
    CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT
    CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT
    GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT
    ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG
    TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA
    ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA
    ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT
    ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC
    ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT
    GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC
    CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT
    CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT
    CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA
    GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT
    AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG
    ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA
    ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT
    CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCCTCATCT
    CCAGTCCCTT ACCTTTCGCC TGAAACAACC CCAGCATCAT CAGCCTCTGC AGTTAATCAT
    AATGTCAGTG TAACTAATGT CAGCCTGGAG GAGCCTGCCC CAGCCCCTCC CACCTCGCAC
    GCATCACAGC CTGGTTGTTT AGGAGCACCA AGTGCTGAGG CAGCTCACGT AGTGCTCAGA
    GGTGAAGGAC CACCATTGCC GCCGCATGCA GACCCAGCAA AGGTGTACAG GAAGGAGCCG
    TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA
    GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA
    AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA
    GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT
    GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT
    CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT
    TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG
    CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC
    AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC
    AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC
    TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT
    TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA
    ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC
    AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT
    GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT
    AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG
    CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT
    GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG
    AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT
    TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAGG GAAAACCCGA AACTGATGCT
    GTGGATAGAT CATTCAGTGA GAAACGTTAT GATCCAGCCC AGGCCACGCC TCCTCCTCCT
    CCGTTGCCCT CACAGTACAG CCAGCCAGCT CCACCTCTGT CCAGCTCTTC TCTCCACATA
    CATTCCAAGG GCGCCCAGGG TGAAGGCAAC TCAGTATCAT TGGATTTTCA GAACTCATAT
    ATGTCCAAAC CAGACCCACC CCCATCTCAG AGTAAACCAG CAACTTTCAG ACCACCAACT
    CGAGAAGACC CCCCTCAGAC TTTCTATCCG CAGAAAAGTT TCCCAGACAA AGCTCCAGTT
    AACGGAGCTG AGCAGACTCA GAAAACCATC ACTCCGGTGT ACAATCGATT CACACCAAAG
    CCGTACACAA GTTCTGCCCG GCCATTTGAA CGCAAATTTG AAAGTCCGAA GTTCAACCAT
    AATCTTCTGC CAAGTGAAAC TGTACATAAA CCTGAATTGT CTTCAAAAAC TCCCACTTCC
    CCAAAAACTC TTATGAAAGC TCATAGTTCA ACACAGCCTC CAGAGTTTGA CAGTGGAGTT
    GAAACTTTCT CTGTTCACAC AGATAAGCCT AAATATCAAA TGAATAATAT CAGCACCATG
    CCTAAAGCTG TCCCTGTGAG TCCTTCAGCT GTGGAAGAAG ATGAAGATGA GGATGGTCAT
    ACTGTAGTGG CTACAGCTCG TGGCATTTTT AACAGCAATG GTGGTGTGTT GAGTTCCATA
    GAAACCGGTG TTAGTATAAT TATCCCACAA GGAGCCATTC CTGAAGGAAT TGAGCAAGAA
    ATCTATTTCA AAGTCTGCAG AGACAATAGC ATTCTCCCAC CTTTAGATAA AGAGAAAGGT
    GAAACTCTGC TGAGCCCCCT AGTGATGTGT GGGCCCCATG GCCTCAAGTT CCTGAAGCCC
    GTGGAGCTAC GCTTGCCACA CTGTGACCCT AAAACCTGGC AAAACAAGTG TCTTCCTGGA
    GATCCGAATT ACCTTGTTGG AGCCAACTGT GTTTCTGTCC TGATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu38773
    Clone ID Related Accession (Same CDS sequence) XM_006540783.4 , XM_006540783.3
    Accession Version XM_006540783.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5388bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product tight junction protein ZO-1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006540783.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT
    CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA
    CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC
    AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA
    CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA
    AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT
    GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG
    AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG
    TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC
    ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT
    GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT
    GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA
    CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC
    TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT
    GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA
    CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG
    CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT
    AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG
    GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT
    GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT
    CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC
    CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT
    CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT
    GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT
    ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG
    TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA
    ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA
    ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT
    ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC
    ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT
    GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC
    CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT
    CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT
    CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA
    GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT
    AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG
    ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA
    ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT
    CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGGTGTACAG GAAGGAGCCG
    TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA
    GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA
    AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA
    GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT
    GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT
    CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT
    TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG
    CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC
    AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC
    AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC
    TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT
    TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA
    ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC
    AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT
    GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT
    AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG
    CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT
    GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG
    AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT
    TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAAT TTCTTGGCTC TTACACTAGC
    TATGACTACT TGAAAAGGAA CATCAAGTGG GGAAAACCCG AAACTGATGC TGTGGATAGA
    TCATTCAGTG AGAAACGTTA TGATCCAGCC CAGGCCACGC CTCCTCCTCC TCCGTTGCCC
    TCACAGTACA GCCAGCCAGC TCCACCTCTG TCCAGCTCTT CTCTCCACAT ACATTCCAAG
    GGCGCCCAGG GTGAAGGCAA CTCAGTATCA TTGGATTTTC AGAACTCATA TATGTCCAAA
    CCAGACCCAC CCCCATCTCA GAGTAAACCA GCAACTTTCA GACCACCAAC TCGAGAAGAC
    CCCCCTCAGA CTTTCTATCC GCAGAAAAGT TTCCCAGACA AAGCTCCAGT TAACGGAGCT
    GAGCAGACTC AGAAAACCAT CACTCCGGTG TACAATCGAT TCACACCAAA GCCGTACACA
    AGTTCTGCCC GGCCATTTGA ACGCAAATTT GAAAGTCCGA AGTTCAACCA TAATCTTCTG
    CCAAGTGAAA CTGTACATAA ACCTGAATTG TCTTCAAAAA CTCCCACTTC CCCAAAAACT
    CTTATGAAAG CTCATAGTTC AACACAGCCT CCAGAGTTTG ACAGTGGAGT TGAAACTTTC
    TCTGTTCACA CAGATAAGCC TAAATATCAA ATGAATAATA TCAGCACCAT GCCTAAAGCT
    GTCCCTGTGA GTCCTTCAGC TGTGGAAGAA GATGAAGATG AGGATGGTCA TACTGTAGTG
    GCTACAGCTC GTGGCATTTT TAACAGCAAT GGTGGTGTGT TGAGTTCCAT AGAAACCGGT
    GTTAGTATAA TTATCCCACA AGGAGCCATT CCTGAAGGAA TTGAGCAAGA AATCTATTTC
    AAAGTCTGCA GAGACAATAG CATTCTCCCA CCTTTAGATA AAGAGAAAGG TGAAACTCTG
    CTGAGCCCCC TAGTGATGTG TGGGCCCCAT GGCCTCAAGT TCCTGAAGCC CGTGGAGCTA
    CGCTTGCCAC ACTGTGCGTC CATGACTCCT GACGGTTGGT CTTTTGCTCT AAAATCATCC
    GACTCCTCGT CGGGTGACCC TAAAACCTGG CAAAACAAGT GTCTTCCTGG AGATCCGAAT
    TACCTTGTTG GAGCCAACTG TGTTTCTGTC CTGATTGACC ACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006540846.1
    CDS115..5502
    Translation

    Target ORF information:

    RefSeq Version XM_006540783.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus tight junction protein 1 (Tjp1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006540783.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT
    CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA
    CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC
    AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA
    CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA
    AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT
    GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG
    AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG
    TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC
    ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT
    GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT
    GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA
    CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC
    TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT
    GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA
    CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG
    CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT
    AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG
    GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT
    GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT
    CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC
    CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT
    CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT
    GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT
    ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG
    TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA
    ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA
    ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT
    ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC
    ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT
    GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC
    CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT
    CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT
    CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA
    GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT
    AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG
    ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA
    ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT
    CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGGTGTACAG GAAGGAGCCG
    TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA
    GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA
    AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA
    GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT
    GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT
    CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT
    TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG
    CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC
    AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC
    AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC
    TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT
    TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA
    ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC
    AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT
    GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT
    AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG
    CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT
    GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG
    AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT
    TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAAT TTCTTGGCTC TTACACTAGC
    TATGACTACT TGAAAAGGAA CATCAAGTGG GGAAAACCCG AAACTGATGC TGTGGATAGA
    TCATTCAGTG AGAAACGTTA TGATCCAGCC CAGGCCACGC CTCCTCCTCC TCCGTTGCCC
    TCACAGTACA GCCAGCCAGC TCCACCTCTG TCCAGCTCTT CTCTCCACAT ACATTCCAAG
    GGCGCCCAGG GTGAAGGCAA CTCAGTATCA TTGGATTTTC AGAACTCATA TATGTCCAAA
    CCAGACCCAC CCCCATCTCA GAGTAAACCA GCAACTTTCA GACCACCAAC TCGAGAAGAC
    CCCCCTCAGA CTTTCTATCC GCAGAAAAGT TTCCCAGACA AAGCTCCAGT TAACGGAGCT
    GAGCAGACTC AGAAAACCAT CACTCCGGTG TACAATCGAT TCACACCAAA GCCGTACACA
    AGTTCTGCCC GGCCATTTGA ACGCAAATTT GAAAGTCCGA AGTTCAACCA TAATCTTCTG
    CCAAGTGAAA CTGTACATAA ACCTGAATTG TCTTCAAAAA CTCCCACTTC CCCAAAAACT
    CTTATGAAAG CTCATAGTTC AACACAGCCT CCAGAGTTTG ACAGTGGAGT TGAAACTTTC
    TCTGTTCACA CAGATAAGCC TAAATATCAA ATGAATAATA TCAGCACCAT GCCTAAAGCT
    GTCCCTGTGA GTCCTTCAGC TGTGGAAGAA GATGAAGATG AGGATGGTCA TACTGTAGTG
    GCTACAGCTC GTGGCATTTT TAACAGCAAT GGTGGTGTGT TGAGTTCCAT AGAAACCGGT
    GTTAGTATAA TTATCCCACA AGGAGCCATT CCTGAAGGAA TTGAGCAAGA AATCTATTTC
    AAAGTCTGCA GAGACAATAG CATTCTCCCA CCTTTAGATA AAGAGAAAGG TGAAACTCTG
    CTGAGCCCCC TAGTGATGTG TGGGCCCCAT GGCCTCAAGT TCCTGAAGCC CGTGGAGCTA
    CGCTTGCCAC ACTGTGCGTC CATGACTCCT GACGGTTGGT CTTTTGCTCT AAAATCATCC
    GACTCCTCGT CGGGTGACCC TAAAACCTGG CAAAACAAGT GTCTTCCTGG AGATCCGAAT
    TACCTTGTTG GAGCCAACTG TGTTTCTGTC CTGATTGACC ACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu38774
    Clone ID Related Accession (Same CDS sequence) XM_006540784.4 , XM_006540784.3
    Accession Version XM_006540784.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5349bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product tight junction protein ZO-1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006540784.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGTCCGCCA GGGCCGCGGC CGCTAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
    CAGCACACAG TGACGCTTCA CAGGGCTCCT GGGTTTGGAT TTGGAATTGC AATCTCTGGT
    GGAAGAGATA ATCCTCATTT TCAGAGTGGG GAAACCTCCA TAGTGATTTC TGATGTGTTA
    AAAGGAGGGC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA
    GTTTCAATGG ATAACGTTGA ACATGCTTTT GCTGTTCAGC AGCTAAGGAA GAGTGGGAAA
    AACGCAAAAA TTACTATCCG AAGGAAGAAG AAAGTTCAGA TCCCTGTAAG TCACCCAGAT
    CCTGAGCCGG TGTCTGATAA TGAAGACGAT AGTTATGACG AAGAAGTGCA TGACCCAAGA
    GCTGGCCGCG GTGCTTTAGC GAACAGAAGG AGCGAGAAGA GCTGGGCAAG GGATAGGAGT
    GCAAGCAGGG AGAGGAGCCT GTCCCCTCGC TCGGACAGGC GGTCCGTGGC CTCCAGTCAG
    CCCGCAAAGC CCACCAAGGT CACACTGGTG AAGTCTCGGA AAAATGAAGA ATATGGTCTT
    CGATTGGCCA GCCATATATT TGTAAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT
    GGTAACATTC AAGAAGGGGA TGTTGTCTTG AAGATAAATG GTACTGTGAC AGAAAATATG
    TCATTGACAG ATGCAAAAAC ACTGATAGAA AGGTCTAAAG GCAAGTTAAA AATGGTAGTG
    CAAAGAGATG AGCGGGCTAC CTTACTGAAC GTCCCTGACC TTTCGGATAG TATCCATTCT
    GCTAATGCCT CGGAAAGAGA TGACATTTCA GAAATTCAGT CACTAGCATC AGACCATTCA
    GGTCGCTCGC ATGACAGGCC ACCCCGCCGC AGCCAGTCAC GATCTCCTGA CCAACGTTCA
    GAGCCCTCCG ATCATTCCAC GCAGTCTCCA CAGCAGCCCA GCAATGGCAG TCTCCGGAGC
    AGAGAGGAAG AGCGAATGTC TAAACCTGGG GCCATCTCAA CTCCTGTAAA ACATGTAGAC
    GATCATCCAC CCAAAGCAGT GGAAGAAGTT ACAGTTGAGA AAAATGAGAA GCAGACGCCC
    ACTCTTCCAG AACCGAAACC TGTGTATGCT CAAGTTGGAC AACCAGATGT GGATTTACCC
    GTCAGCCCTT CTGATGGTGC TCTGCCTAAT TCAGCTCATG AAGACGGGAT ACTTAGGCCC
    AGCATGAAAC TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGACT AGCTGGTGGA
    AATGATGTCG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAAGAAGGC
    TTAGAGGAAG GTGATCAAAT TCTCAGGGTG AACAATGTAG ATTTCACAAA TATCATAAGG
    GAAGAGGCCG TCCTTTTCCT CCTTGACCTC CCTAAAGGTG AAGAAGTGAC CATACTGGCT
    CAGAAGAAGA AGGACGTTTA TCGCCGCATT GTAGAATCAG ATGTAGGAGA TTCATTCTAT
    ATTAGAACGC ATTTTGAATA TGAAAAAGAA TCTCCTTACG GACTTAGTTT TAACAAAGGA
    GAGGTGTTCC GGGTCGTGGA TACTTTATAC AATGGAAAGC TGGGCTCTTG GCTTGCCATT
    CGAATTGGCA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT
    GAACAGTTAG CCAGTGTACA GTACACACTT CCAAAGACAG CGGGTGGTGA TCGGGCAGAC
    TTCTGGAGGT TTCGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAC
    TTGTCAGCTC AGCCAGTTCA AACTAAGTTC CCAGCTTATG AAAGGGTTGT TCTTCGAGAA
    GCTGGATTCC TAAGACCTGT AACCATCTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG
    TTGGCAAGAG AGGAGCCAGA TATCTATCAG ATTGCAAAAA GTGAACCACG AGATGCTGGG
    ACTGACCATC GTAGCTCTGG CATCATTCGC CTTCATACAA TAAAGCAAAT CATAGATCAG
    GATAAACATG CTTTATTAGA TGTAACGCCA AATGCGGTTG ATCGTCTTAA TTATGCGCAG
    TGGTATCCAA TTGTTGTGTT CCTTAACCCT GACTCTAAGC AAGGTGTAAA AACAATGAGG
    ATGAGGTTGT GTCCGGAGTC TCGGAAAAGC GCCAGGAAGC TATATGAACG CTCTCATAAG
    CTTCGTAAGA ACAATCACCA TCTCTTCACA ACTACAATTA ACTTAAACTC AATGAATGAT
    GGTTGGTACG GTGCCCTGAA AGAAGCGATT CAGCAGCAAC AGAACCAGCT GGTGTGGGTC
    TCTGAGGGGA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA CGATCGTCTG
    TCCTACCTGT CAGCCCCAGG TAGTGAGTAC TCAATGTATA GCACGGACAG TAGACACACT
    TCTGACTATG AAGACACAGA TACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA
    ACTCTTAATG ATGAGGTGGG GACTCCCCCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT
    GTAAGAGAGG ATTCCTCTGG AATGCATCAT GAAAACCAGA CATACCCTCC TTACTCACCA
    CAAGCGCAGC CACAAGCTAT TCATAGAATA GACTCCCCTG GACTTAAGCC AGCCTCTCAA
    CAGAAAGCAG AAGCCTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAC CCCAGCATCA
    TCAGCCTCTG CAGTTAATCA TAATGTCAGT GTAACTAATG TCAGCCTGGA GGAGCCTGCC
    CCAGCCCCTC CCACCTCGCA CGCATCACAG CCTGGTTGTT TAGGAGCACC AAGTGCTGAG
    GCAGCTCACG TAGTGCTCAG AGGTGAAGGA CCACCATTGC CGCCGCATGC AGACCCAGCA
    AAGGTGTACA GGAAGGAGCC GTATTCTGAA GAAATGATGA GACAAAACCA TATTTTAAAA
    CAACCAGCTC TTGGTCACCC AGGGCAGAGG CCAGATAAAG AGCCAAATCT AGCCTATGAA
    CCCCAACTTC CATATATAGA AAAACAAGCC AGCAGAGACC TTGAGCAGCC GTCATACAGG
    TATGAGGTCT CAAGCTACAC AGACCAGTTT TCTCGGAACT ATGACCATCG CCTACGGTTT
    GAAGATCGAA TCCCTACCTA TGAAGACCAG TGGTCATATT ATGATGACAA ACAGCCCTAC
    CAACCTCGGC CTTTTGAGAA TCAGCATCCC CGAGACCTGG ACTCCAGACA ACATCCCGAA
    GAGGCTTCAG AACGAGGTTA TTTCCAGCGT TTTGAAGAGC CAGCCCCTCT GTCGTACGAC
    AGTAGAACAC GCTATGAGCA GCTGCCTCGA ACCTCTACTC TACGACATGA AGAGCAGCCA
    GCCCCTGCAT ATGAGGTGCA CAACAGGTAC AGGCCAGAGG CACAGCCCTA TTCTTCAACA
    GGCCCTAAGT CATCTGAGCC CAAGCAGTAC TTTGACCAGT ACCCGCGAAG TTATGAGCAA
    GTTCCACCAC CAGGCTTTAC CTCCAAAACA GGCCATTACG AGCCTCTCCA TGGTGCTGCA
    GTTGTCCCTC CTCTGATACC TTCCTCTCAA CAAAAGCCAG AAGTCCTGCC CTCGGCTACC
    AAACCACAGC CTCCACCCCC AACCCTAACT GAGGAGGAGG AGGATCCAGC AATGAAACCA
    CAGTCTGTGC TCACCAGAGT CAAAATGTTT GAAAACAAAA GATCTGCGTC TTTGGAGAAC
    AAGAAAGATG TGAATGACAC TGCCAGCTTT AAGCCTCCGG AAGTAGCATC TAAACCTCCA
    GGTGCTTCTC TTGCTGGCCC TAAACCTGTC CCTCAGAGTC AGTTTAGTGA GCACGACAAA
    ACGCTCTACA GGCTCCCAGA GCCTCAGAAA CCTCAAGTGA AGCCACCCGA AGATATTGTT
    CGATCAAATC ATTACGACCC TGAAGAGGAT GAAGAATATT ACCGGAAACA GCTCTCCTAC
    TTTGACCGAA GAAGTTTTGA GAGCAAGCCT TCTGCACATC TTCCTGCTGG CCATCACTCA
    GAGCCTGCTA AGCCAGTCCA TTCTCAGAGC CAGCCCAACT TCTCTAGTTA CTCTTCAAAA
    TTTCTTGGCT CTTACACTAG CTATGACTAC TTGAAAAGGA ACATCAAGTG GGGAAAACCC
    GAAACTGATG CTGTGGATAG ATCATTCAGT GAGAAACGTT ATGATCCAGC CCAGGCCACG
    CCTCCTCCTC CTCCGTTGCC CTCACAGTAC AGCCAGCCAG CTCCACCTCT GTCCAGCTCT
    TCTCTCCACA TACATTCCAA GGGCGCCCAG GGTGAAGGCA ACTCAGTATC ATTGGATTTT
    CAGAACTCAT ATATGTCCAA ACCAGACCCA CCCCCATCTC AGAGTAAACC AGCAACTTTC
    AGACCACCAA CTCGAGAAGA CCCCCCTCAG ACTTTCTATC CGCAGAAAAG TTTCCCAGAC
    AAAGCTCCAG TTAACGGAGC TGAGCAGACT CAGAAAACCA TCACTCCGGT GTACAATCGA
    TTCACACCAA AGCCGTACAC AAGTTCTGCC CGGCCATTTG AACGCAAATT TGAAAGTCCG
    AAGTTCAACC ATAATCTTCT GCCAAGTGAA ACTGTACATA AACCTGAATT GTCTTCAAAA
    ACTCCCACTT CCCCAAAAAC TCTTATGAAA GCTCATAGTT CAACACAGCC TCCAGAGTTT
    GACAGTGGAG TTGAAACTTT CTCTGTTCAC ACAGATAAGC CTAAATATCA AATGAATAAT
    ATCAGCACCA TGCCTAAAGC TGTCCCTGTG AGTCCTTCAG CTGTGGAAGA AGATGAAGAT
    GAGGATGGTC ATACTGTAGT GGCTACAGCT CGTGGCATTT TTAACAGCAA TGGTGGTGTG
    TTGAGTTCCA TAGAAACCGG TGTTAGTATA ATTATCCCAC AAGGAGCCAT TCCTGAAGGA
    ATTGAGCAAG AAATCTATTT CAAAGTCTGC AGAGACAATA GCATTCTCCC ACCTTTAGAT
    AAAGAGAAAG GTGAAACTCT GCTGAGCCCC CTAGTGATGT GTGGGCCCCA TGGCCTCAAG
    TTCCTGAAGC CCGTGGAGCT ACGCTTGCCA CACTGTGCGT CCATGACTCC TGACGGTTGG
    TCTTTTGCTC TAAAATCATC CGACTCCTCG TCGGGTGACC CTAAAACCTG GCAAAACAAG
    TGTCTTCCTG GAGATCCGAA TTACCTTGTT GGAGCCAACT GTGTTTCTGT CCTGATTGAC
    CACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006540847.1
    CDS846..6194
    Translation

    Target ORF information:

    RefSeq Version XM_006540784.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus tight junction protein 1 (Tjp1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006540784.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGTCCGCCA GGGCCGCGGC CGCTAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
    CAGCACACAG TGACGCTTCA CAGGGCTCCT GGGTTTGGAT TTGGAATTGC AATCTCTGGT
    GGAAGAGATA ATCCTCATTT TCAGAGTGGG GAAACCTCCA TAGTGATTTC TGATGTGTTA
    AAAGGAGGGC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA
    GTTTCAATGG ATAACGTTGA ACATGCTTTT GCTGTTCAGC AGCTAAGGAA GAGTGGGAAA
    AACGCAAAAA TTACTATCCG AAGGAAGAAG AAAGTTCAGA TCCCTGTAAG TCACCCAGAT
    CCTGAGCCGG TGTCTGATAA TGAAGACGAT AGTTATGACG AAGAAGTGCA TGACCCAAGA
    GCTGGCCGCG GTGCTTTAGC GAACAGAAGG AGCGAGAAGA GCTGGGCAAG GGATAGGAGT
    GCAAGCAGGG AGAGGAGCCT GTCCCCTCGC TCGGACAGGC GGTCCGTGGC CTCCAGTCAG
    CCCGCAAAGC CCACCAAGGT CACACTGGTG AAGTCTCGGA AAAATGAAGA ATATGGTCTT
    CGATTGGCCA GCCATATATT TGTAAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT
    GGTAACATTC AAGAAGGGGA TGTTGTCTTG AAGATAAATG GTACTGTGAC AGAAAATATG
    TCATTGACAG ATGCAAAAAC ACTGATAGAA AGGTCTAAAG GCAAGTTAAA AATGGTAGTG
    CAAAGAGATG AGCGGGCTAC CTTACTGAAC GTCCCTGACC TTTCGGATAG TATCCATTCT
    GCTAATGCCT CGGAAAGAGA TGACATTTCA GAAATTCAGT CACTAGCATC AGACCATTCA
    GGTCGCTCGC ATGACAGGCC ACCCCGCCGC AGCCAGTCAC GATCTCCTGA CCAACGTTCA
    GAGCCCTCCG ATCATTCCAC GCAGTCTCCA CAGCAGCCCA GCAATGGCAG TCTCCGGAGC
    AGAGAGGAAG AGCGAATGTC TAAACCTGGG GCCATCTCAA CTCCTGTAAA ACATGTAGAC
    GATCATCCAC CCAAAGCAGT GGAAGAAGTT ACAGTTGAGA AAAATGAGAA GCAGACGCCC
    ACTCTTCCAG AACCGAAACC TGTGTATGCT CAAGTTGGAC AACCAGATGT GGATTTACCC
    GTCAGCCCTT CTGATGGTGC TCTGCCTAAT TCAGCTCATG AAGACGGGAT ACTTAGGCCC
    AGCATGAAAC TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGACT AGCTGGTGGA
    AATGATGTCG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAAGAAGGC
    TTAGAGGAAG GTGATCAAAT TCTCAGGGTG AACAATGTAG ATTTCACAAA TATCATAAGG
    GAAGAGGCCG TCCTTTTCCT CCTTGACCTC CCTAAAGGTG AAGAAGTGAC CATACTGGCT
    CAGAAGAAGA AGGACGTTTA TCGCCGCATT GTAGAATCAG ATGTAGGAGA TTCATTCTAT
    ATTAGAACGC ATTTTGAATA TGAAAAAGAA TCTCCTTACG GACTTAGTTT TAACAAAGGA
    GAGGTGTTCC GGGTCGTGGA TACTTTATAC AATGGAAAGC TGGGCTCTTG GCTTGCCATT
    CGAATTGGCA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT
    GAACAGTTAG CCAGTGTACA GTACACACTT CCAAAGACAG CGGGTGGTGA TCGGGCAGAC
    TTCTGGAGGT TTCGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAC
    TTGTCAGCTC AGCCAGTTCA AACTAAGTTC CCAGCTTATG AAAGGGTTGT TCTTCGAGAA
    GCTGGATTCC TAAGACCTGT AACCATCTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG
    TTGGCAAGAG AGGAGCCAGA TATCTATCAG ATTGCAAAAA GTGAACCACG AGATGCTGGG
    ACTGACCATC GTAGCTCTGG CATCATTCGC CTTCATACAA TAAAGCAAAT CATAGATCAG
    GATAAACATG CTTTATTAGA TGTAACGCCA AATGCGGTTG ATCGTCTTAA TTATGCGCAG
    TGGTATCCAA TTGTTGTGTT CCTTAACCCT GACTCTAAGC AAGGTGTAAA AACAATGAGG
    ATGAGGTTGT GTCCGGAGTC TCGGAAAAGC GCCAGGAAGC TATATGAACG CTCTCATAAG
    CTTCGTAAGA ACAATCACCA TCTCTTCACA ACTACAATTA ACTTAAACTC AATGAATGAT
    GGTTGGTACG GTGCCCTGAA AGAAGCGATT CAGCAGCAAC AGAACCAGCT GGTGTGGGTC
    TCTGAGGGGA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA CGATCGTCTG
    TCCTACCTGT CAGCCCCAGG TAGTGAGTAC TCAATGTATA GCACGGACAG TAGACACACT
    TCTGACTATG AAGACACAGA TACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA
    ACTCTTAATG ATGAGGTGGG GACTCCCCCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT
    GTAAGAGAGG ATTCCTCTGG AATGCATCAT GAAAACCAGA CATACCCTCC TTACTCACCA
    CAAGCGCAGC CACAAGCTAT TCATAGAATA GACTCCCCTG GACTTAAGCC AGCCTCTCAA
    CAGAAAGCAG AAGCCTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAC CCCAGCATCA
    TCAGCCTCTG CAGTTAATCA TAATGTCAGT GTAACTAATG TCAGCCTGGA GGAGCCTGCC
    CCAGCCCCTC CCACCTCGCA CGCATCACAG CCTGGTTGTT TAGGAGCACC AAGTGCTGAG
    GCAGCTCACG TAGTGCTCAG AGGTGAAGGA CCACCATTGC CGCCGCATGC AGACCCAGCA
    AAGGTGTACA GGAAGGAGCC GTATTCTGAA GAAATGATGA GACAAAACCA TATTTTAAAA
    CAACCAGCTC TTGGTCACCC AGGGCAGAGG CCAGATAAAG AGCCAAATCT AGCCTATGAA
    CCCCAACTTC CATATATAGA AAAACAAGCC AGCAGAGACC TTGAGCAGCC GTCATACAGG
    TATGAGGTCT CAAGCTACAC AGACCAGTTT TCTCGGAACT ATGACCATCG CCTACGGTTT
    GAAGATCGAA TCCCTACCTA TGAAGACCAG TGGTCATATT ATGATGACAA ACAGCCCTAC
    CAACCTCGGC CTTTTGAGAA TCAGCATCCC CGAGACCTGG ACTCCAGACA ACATCCCGAA
    GAGGCTTCAG AACGAGGTTA TTTCCAGCGT TTTGAAGAGC CAGCCCCTCT GTCGTACGAC
    AGTAGAACAC GCTATGAGCA GCTGCCTCGA ACCTCTACTC TACGACATGA AGAGCAGCCA
    GCCCCTGCAT ATGAGGTGCA CAACAGGTAC AGGCCAGAGG CACAGCCCTA TTCTTCAACA
    GGCCCTAAGT CATCTGAGCC CAAGCAGTAC TTTGACCAGT ACCCGCGAAG TTATGAGCAA
    GTTCCACCAC CAGGCTTTAC CTCCAAAACA GGCCATTACG AGCCTCTCCA TGGTGCTGCA
    GTTGTCCCTC CTCTGATACC TTCCTCTCAA CAAAAGCCAG AAGTCCTGCC CTCGGCTACC
    AAACCACAGC CTCCACCCCC AACCCTAACT GAGGAGGAGG AGGATCCAGC AATGAAACCA
    CAGTCTGTGC TCACCAGAGT CAAAATGTTT GAAAACAAAA GATCTGCGTC TTTGGAGAAC
    AAGAAAGATG TGAATGACAC TGCCAGCTTT AAGCCTCCGG AAGTAGCATC TAAACCTCCA
    GGTGCTTCTC TTGCTGGCCC TAAACCTGTC CCTCAGAGTC AGTTTAGTGA GCACGACAAA
    ACGCTCTACA GGCTCCCAGA GCCTCAGAAA CCTCAAGTGA AGCCACCCGA AGATATTGTT
    CGATCAAATC ATTACGACCC TGAAGAGGAT GAAGAATATT ACCGGAAACA GCTCTCCTAC
    TTTGACCGAA GAAGTTTTGA GAGCAAGCCT TCTGCACATC TTCCTGCTGG CCATCACTCA
    GAGCCTGCTA AGCCAGTCCA TTCTCAGAGC CAGCCCAACT TCTCTAGTTA CTCTTCAAAA
    TTTCTTGGCT CTTACACTAG CTATGACTAC TTGAAAAGGA ACATCAAGTG GGGAAAACCC
    GAAACTGATG CTGTGGATAG ATCATTCAGT GAGAAACGTT ATGATCCAGC CCAGGCCACG
    CCTCCTCCTC CTCCGTTGCC CTCACAGTAC AGCCAGCCAG CTCCACCTCT GTCCAGCTCT
    TCTCTCCACA TACATTCCAA GGGCGCCCAG GGTGAAGGCA ACTCAGTATC ATTGGATTTT
    CAGAACTCAT ATATGTCCAA ACCAGACCCA CCCCCATCTC AGAGTAAACC AGCAACTTTC
    AGACCACCAA CTCGAGAAGA CCCCCCTCAG ACTTTCTATC CGCAGAAAAG TTTCCCAGAC
    AAAGCTCCAG TTAACGGAGC TGAGCAGACT CAGAAAACCA TCACTCCGGT GTACAATCGA
    TTCACACCAA AGCCGTACAC AAGTTCTGCC CGGCCATTTG AACGCAAATT TGAAAGTCCG
    AAGTTCAACC ATAATCTTCT GCCAAGTGAA ACTGTACATA AACCTGAATT GTCTTCAAAA
    ACTCCCACTT CCCCAAAAAC TCTTATGAAA GCTCATAGTT CAACACAGCC TCCAGAGTTT
    GACAGTGGAG TTGAAACTTT CTCTGTTCAC ACAGATAAGC CTAAATATCA AATGAATAAT
    ATCAGCACCA TGCCTAAAGC TGTCCCTGTG AGTCCTTCAG CTGTGGAAGA AGATGAAGAT
    GAGGATGGTC ATACTGTAGT GGCTACAGCT CGTGGCATTT TTAACAGCAA TGGTGGTGTG
    TTGAGTTCCA TAGAAACCGG TGTTAGTATA ATTATCCCAC AAGGAGCCAT TCCTGAAGGA
    ATTGAGCAAG AAATCTATTT CAAAGTCTGC AGAGACAATA GCATTCTCCC ACCTTTAGAT
    AAAGAGAAAG GTGAAACTCT GCTGAGCCCC CTAGTGATGT GTGGGCCCCA TGGCCTCAAG
    TTCCTGAAGC CCGTGGAGCT ACGCTTGCCA CACTGTGCGT CCATGACTCC TGACGGTTGG
    TCTTTTGCTC TAAAATCATC CGACTCCTCG TCGGGTGACC CTAAAACCTG GCAAAACAAG
    TGTCTTCCTG GAGATCCGAA TTACCTTGTT GGAGCCAACT GTGTTTCTGT CCTGATTGAC
    CACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu38775
    Clone ID Related Accession (Same CDS sequence) XM_006540785.3 , XM_006540785.4
    Accession Version XM_006540785.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5337bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product tight junction protein ZO-1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006540785.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT
    CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA
    CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC
    AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA
    CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA
    AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT
    GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG
    AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG
    TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC
    ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT
    GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT
    GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA
    CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC
    TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT
    GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA
    CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG
    CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT
    AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG
    GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT
    GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT
    CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC
    CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT
    CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT
    GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT
    ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG
    TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA
    ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA
    ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT
    ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC
    ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT
    GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC
    CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT
    CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT
    CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA
    GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT
    AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG
    ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA
    ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT
    CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGGTGTACAG GAAGGAGCCG
    TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA
    GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA
    AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA
    GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT
    GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT
    CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT
    TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG
    CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC
    AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC
    AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC
    TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT
    TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA
    ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC
    AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT
    GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT
    AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG
    CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT
    GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG
    AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT
    TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAGG GAAAACCCGA AACTGATGCT
    GTGGATAGAT CATTCAGTGA GAAACGTTAT GATCCAGCCC AGGCCACGCC TCCTCCTCCT
    CCGTTGCCCT CACAGTACAG CCAGCCAGCT CCACCTCTGT CCAGCTCTTC TCTCCACATA
    CATTCCAAGG GCGCCCAGGG TGAAGGCAAC TCAGTATCAT TGGATTTTCA GAACTCATAT
    ATGTCCAAAC CAGACCCACC CCCATCTCAG AGTAAACCAG CAACTTTCAG ACCACCAACT
    CGAGAAGACC CCCCTCAGAC TTTCTATCCG CAGAAAAGTT TCCCAGACAA AGCTCCAGTT
    AACGGAGCTG AGCAGACTCA GAAAACCATC ACTCCGGTGT ACAATCGATT CACACCAAAG
    CCGTACACAA GTTCTGCCCG GCCATTTGAA CGCAAATTTG AAAGTCCGAA GTTCAACCAT
    AATCTTCTGC CAAGTGAAAC TGTACATAAA CCTGAATTGT CTTCAAAAAC TCCCACTTCC
    CCAAAAACTC TTATGAAAGC TCATAGTTCA ACACAGCCTC CAGAGTTTGA CAGTGGAGTT
    GAAACTTTCT CTGTTCACAC AGATAAGCCT AAATATCAAA TGAATAATAT CAGCACCATG
    CCTAAAGCTG TCCCTGTGAG TCCTTCAGCT GTGGAAGAAG ATGAAGATGA GGATGGTCAT
    ACTGTAGTGG CTACAGCTCG TGGCATTTTT AACAGCAATG GTGGTGTGTT GAGTTCCATA
    GAAACCGGTG TTAGTATAAT TATCCCACAA GGAGCCATTC CTGAAGGAAT TGAGCAAGAA
    ATCTATTTCA AAGTCTGCAG AGACAATAGC ATTCTCCCAC CTTTAGATAA AGAGAAAGGT
    GAAACTCTGC TGAGCCCCCT AGTGATGTGT GGGCCCCATG GCCTCAAGTT CCTGAAGCCC
    GTGGAGCTAC GCTTGCCACA CTGTGCGTCC ATGACTCCTG ACGGTTGGTC TTTTGCTCTA
    AAATCATCCG ACTCCTCGTC GGGTGACCCT AAAACCTGGC AAAACAAGTG TCTTCCTGGA
    GATCCGAATT ACCTTGTTGG AGCCAACTGT GTTTCTGTCC TGATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006540848.1
    CDS115..5451
    Translation

    Target ORF information:

    RefSeq Version XM_006540785.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus tight junction protein 1 (Tjp1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006540785.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT
    CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA
    CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC
    AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA
    CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA
    AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT
    GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG
    AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG
    TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC
    ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT
    GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT
    GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA
    CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC
    TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT
    GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA
    CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG
    CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT
    AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG
    GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT
    GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT
    CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC
    CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT
    CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT
    GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT
    ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG
    TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA
    ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA
    ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT
    ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC
    ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT
    GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC
    CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT
    CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT
    CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA
    GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT
    AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG
    ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA
    ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT
    CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGGTGTACAG GAAGGAGCCG
    TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA
    GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA
    AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA
    GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT
    GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT
    CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT
    TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG
    CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC
    AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC
    AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC
    TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT
    TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA
    ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC
    AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT
    GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT
    AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG
    CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT
    GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG
    AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT
    TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAGG GAAAACCCGA AACTGATGCT
    GTGGATAGAT CATTCAGTGA GAAACGTTAT GATCCAGCCC AGGCCACGCC TCCTCCTCCT
    CCGTTGCCCT CACAGTACAG CCAGCCAGCT CCACCTCTGT CCAGCTCTTC TCTCCACATA
    CATTCCAAGG GCGCCCAGGG TGAAGGCAAC TCAGTATCAT TGGATTTTCA GAACTCATAT
    ATGTCCAAAC CAGACCCACC CCCATCTCAG AGTAAACCAG CAACTTTCAG ACCACCAACT
    CGAGAAGACC CCCCTCAGAC TTTCTATCCG CAGAAAAGTT TCCCAGACAA AGCTCCAGTT
    AACGGAGCTG AGCAGACTCA GAAAACCATC ACTCCGGTGT ACAATCGATT CACACCAAAG
    CCGTACACAA GTTCTGCCCG GCCATTTGAA CGCAAATTTG AAAGTCCGAA GTTCAACCAT
    AATCTTCTGC CAAGTGAAAC TGTACATAAA CCTGAATTGT CTTCAAAAAC TCCCACTTCC
    CCAAAAACTC TTATGAAAGC TCATAGTTCA ACACAGCCTC CAGAGTTTGA CAGTGGAGTT
    GAAACTTTCT CTGTTCACAC AGATAAGCCT AAATATCAAA TGAATAATAT CAGCACCATG
    CCTAAAGCTG TCCCTGTGAG TCCTTCAGCT GTGGAAGAAG ATGAAGATGA GGATGGTCAT
    ACTGTAGTGG CTACAGCTCG TGGCATTTTT AACAGCAATG GTGGTGTGTT GAGTTCCATA
    GAAACCGGTG TTAGTATAAT TATCCCACAA GGAGCCATTC CTGAAGGAAT TGAGCAAGAA
    ATCTATTTCA AAGTCTGCAG AGACAATAGC ATTCTCCCAC CTTTAGATAA AGAGAAAGGT
    GAAACTCTGC TGAGCCCCCT AGTGATGTGT GGGCCCCATG GCCTCAAGTT CCTGAAGCCC
    GTGGAGCTAC GCTTGCCACA CTGTGCGTCC ATGACTCCTG ACGGTTGGTC TTTTGCTCTA
    AAATCATCCG ACTCCTCGTC GGGTGACCCT AAAACCTGGC AAAACAAGTG TCTTCCTGGA
    GATCCGAATT ACCTTGTTGG AGCCAACTGT GTTTCTGTCC TGATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu38776
    Clone ID Related Accession (Same CDS sequence) XM_006540786.4 , XM_006540786.3
    Accession Version XM_006540786.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5277bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product tight junction protein ZO-1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006540786.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT
    CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA
    CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC
    AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA
    CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA
    AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT
    GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG
    AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG
    TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC
    ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT
    GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT
    GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA
    CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC
    TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT
    GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA
    CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG
    CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT
    AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG
    GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT
    GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT
    CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC
    CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT
    CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT
    GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT
    ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG
    TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA
    ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA
    ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT
    ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC
    ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT
    GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC
    CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT
    CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT
    CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA
    GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT
    AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG
    ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA
    ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT
    CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGGTGTACAG GAAGGAGCCG
    TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA
    GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA
    AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA
    GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT
    GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT
    CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT
    TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG
    CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC
    AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC
    AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC
    TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT
    TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA
    ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC
    AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT
    GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT
    AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG
    CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT
    GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG
    AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT
    TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAGG GAAAACCCGA AACTGATGCT
    GTGGATAGAT CATTCAGTGA GAAACGTTAT GATCCAGCCC AGGCCACGCC TCCTCCTCCT
    CCGTTGCCCT CACAGTACAG CCAGCCAGCT CCACCTCTGT CCAGCTCTTC TCTCCACATA
    CATTCCAAGG GCGCCCAGGG TGAAGGCAAC TCAGTATCAT TGGATTTTCA GAACTCATAT
    ATGTCCAAAC CAGACCCACC CCCATCTCAG AGTAAACCAG CAACTTTCAG ACCACCAACT
    CGAGAAGACC CCCCTCAGAC TTTCTATCCG CAGAAAAGTT TCCCAGACAA AGCTCCAGTT
    AACGGAGCTG AGCAGACTCA GAAAACCATC ACTCCGGTGT ACAATCGATT CACACCAAAG
    CCGTACACAA GTTCTGCCCG GCCATTTGAA CGCAAATTTG AAAGTCCGAA GTTCAACCAT
    AATCTTCTGC CAAGTGAAAC TGTACATAAA CCTGAATTGT CTTCAAAAAC TCCCACTTCC
    CCAAAAACTC TTATGAAAGC TCATAGTTCA ACACAGCCTC CAGAGTTTGA CAGTGGAGTT
    GAAACTTTCT CTGTTCACAC AGATAAGCCT AAATATCAAA TGAATAATAT CAGCACCATG
    CCTAAAGCTG TCCCTGTGAG TCCTTCAGCT GTGGAAGAAG ATGAAGATGA GGATGGTCAT
    ACTGTAGTGG CTACAGCTCG TGGCATTTTT AACAGCAATG GTGGTGTGTT GAGTTCCATA
    GAAACCGGTG TTAGTATAAT TATCCCACAA GGAGCCATTC CTGAAGGAAT TGAGCAAGAA
    ATCTATTTCA AAGTCTGCAG AGACAATAGC ATTCTCCCAC CTTTAGATAA AGAGAAAGGT
    GAAACTCTGC TGAGCCCCCT AGTGATGTGT GGGCCCCATG GCCTCAAGTT CCTGAAGCCC
    GTGGAGCTAC GCTTGCCACA CTGTGACCCT AAAACCTGGC AAAACAAGTG TCTTCCTGGA
    GATCCGAATT ACCTTGTTGG AGCCAACTGT GTTTCTGTCC TGATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006540849.1
    CDS115..5391
    Translation

    Target ORF information:

    RefSeq Version XM_006540786.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus tight junction protein 1 (Tjp1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006540786.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT
    CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA
    CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC
    AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA
    CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA
    AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT
    GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG
    AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG
    TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC
    ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT
    GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT
    GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA
    CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC
    TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT
    GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA
    CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG
    CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT
    AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG
    GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT
    GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT
    CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC
    CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT
    CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT
    GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT
    ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG
    TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA
    ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA
    ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT
    ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC
    ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT
    GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC
    CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT
    CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT
    CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA
    GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT
    AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG
    ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA
    ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT
    CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGGTGTACAG GAAGGAGCCG
    TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA
    GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA
    AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA
    GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT
    GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT
    CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT
    TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG
    CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC
    AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC
    AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC
    TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT
    TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA
    ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC
    AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT
    GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT
    AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG
    CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT
    GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG
    AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT
    TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAGG GAAAACCCGA AACTGATGCT
    GTGGATAGAT CATTCAGTGA GAAACGTTAT GATCCAGCCC AGGCCACGCC TCCTCCTCCT
    CCGTTGCCCT CACAGTACAG CCAGCCAGCT CCACCTCTGT CCAGCTCTTC TCTCCACATA
    CATTCCAAGG GCGCCCAGGG TGAAGGCAAC TCAGTATCAT TGGATTTTCA GAACTCATAT
    ATGTCCAAAC CAGACCCACC CCCATCTCAG AGTAAACCAG CAACTTTCAG ACCACCAACT
    CGAGAAGACC CCCCTCAGAC TTTCTATCCG CAGAAAAGTT TCCCAGACAA AGCTCCAGTT
    AACGGAGCTG AGCAGACTCA GAAAACCATC ACTCCGGTGT ACAATCGATT CACACCAAAG
    CCGTACACAA GTTCTGCCCG GCCATTTGAA CGCAAATTTG AAAGTCCGAA GTTCAACCAT
    AATCTTCTGC CAAGTGAAAC TGTACATAAA CCTGAATTGT CTTCAAAAAC TCCCACTTCC
    CCAAAAACTC TTATGAAAGC TCATAGTTCA ACACAGCCTC CAGAGTTTGA CAGTGGAGTT
    GAAACTTTCT CTGTTCACAC AGATAAGCCT AAATATCAAA TGAATAATAT CAGCACCATG
    CCTAAAGCTG TCCCTGTGAG TCCTTCAGCT GTGGAAGAAG ATGAAGATGA GGATGGTCAT
    ACTGTAGTGG CTACAGCTCG TGGCATTTTT AACAGCAATG GTGGTGTGTT GAGTTCCATA
    GAAACCGGTG TTAGTATAAT TATCCCACAA GGAGCCATTC CTGAAGGAAT TGAGCAAGAA
    ATCTATTTCA AAGTCTGCAG AGACAATAGC ATTCTCCCAC CTTTAGATAA AGAGAAAGGT
    GAAACTCTGC TGAGCCCCCT AGTGATGTGT GGGCCCCATG GCCTCAAGTT CCTGAAGCCC
    GTGGAGCTAC GCTTGCCACA CTGTGACCCT AAAACCTGGC AAAACAAGTG TCTTCCTGGA
    GATCCGAATT ACCTTGTTGG AGCCAACTGT GTTTCTGTCC TGATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu68895
    Clone ID Related Accession (Same CDS sequence) XM_011250843.2 , XM_011250843.3
    Accession Version XM_011250843.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5538bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product tight junction protein ZO-1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011250843.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT
    CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA
    CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC
    AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA
    CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA
    AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT
    GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG
    AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG
    TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC
    ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT
    GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT
    GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA
    CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC
    TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT
    GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA
    CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG
    CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT
    AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG
    GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT
    GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT
    CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC
    CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT
    CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT
    GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT
    ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG
    TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA
    ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA
    ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT
    ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC
    ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT
    GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC
    CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT
    CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT
    CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA
    GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT
    AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG
    ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA
    ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT
    CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCCTCATCT
    CCAGTCCCTT ACCTTTCGCC TGAAACAACC CCAGCATCAT CAGCCTCTGC AGTTAATCAT
    AATGTCAGTG TAACTAATGT CAGCCTGGAG GAGCCTGCCC CAGCCCCTCC CACCTCGCAC
    GCATCACAGC CTGGTTGTTT AGGAGCACCA AGTGCTGAGG CAGCTCACGT AGTGCTCAGA
    GGTGAAGGAC CACCATTGCC GCCGCATGCA GACCCAGCAA AGGTGTACAG GAAGGAGCCG
    TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA
    GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA
    AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA
    GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT
    GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT
    CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT
    TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG
    CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC
    AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC
    AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC
    TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT
    TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA
    ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC
    AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT
    GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT
    AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG
    CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT
    GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG
    AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT
    TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAGG GAAAACCCGA AACTGATGCT
    GTGGATAGAT CATTCAGTGA GAAACGTTAT GATCCAGCCC AGGCCACGCC TCCTCCTCCT
    CCGTTGCCCT CACAGTACAG CCAGCCAGCT CCACCTCTGT CCAGCTCTTC TCTCCACATA
    CATTCCAAGG GCGCCCAGGG TGAAGGCAAC TCAGTATCAT TGGATTTTCA GAACTCATAT
    ATGTCCAAAC CAGACCCACC CCCATCTCAG AGTAAACCAG CAACTTTCAG ACCACCAACT
    CGAGAAGACC CCCCTCAGAC TTTCTATCCG CAGAAAAGTT TCCCAGACAA AGCTCCAGTT
    AACGGAGCTG AGCAGACTCA GAAAACCATC ACTCCGGTGT ACAATCGATT CACACCAAAG
    CCGTACACAA GTTCTGCCCG GCCATTTGAA CGCAAATTTG AAAGTCCGAA GTTCAACCAT
    AATCTTCTGC CAAGTGAAAC TGTACATAAA CCTGAATTGT CTTCAAAAAC TCCCACTTCC
    CCAAAAACTC TTATGAAAGC TCATAGTTCA ACACAGCCTC CAGAGTTTGA CAGTGGAGTT
    GAAACTTTCT CTGTTCACAC AGATAAGCCT AAATATCAAA TGAATAATAT CAGCACCATG
    CCTAAAGCTG TCCCTGTGAG TCCTTCAGCT GTGGAAGAAG ATGAAGATGA GGATGGTCAT
    ACTGTAGTGG CTACAGCTCG TGGCATTTTT AACAGCAATG GTGGTGTGTT GAGTTCCATA
    GAAACCGGTG TTAGTATAAT TATCCCACAA GGAGCCATTC CTGAAGGAAT TGAGCAAGAA
    ATCTATTTCA AAGTCTGCAG AGACAATAGC ATTCTCCCAC CTTTAGATAA AGAGAAAGGT
    GAAACTCTGC TGAGCCCCCT AGTGATGTGT GGGCCCCATG GCCTCAAGTT CCTGAAGCCC
    GTGGAGCTAC GCTTGCCACA CTGTGCGTCC ATGACTCCTG ACGGTGACCC TAAAACCTGG
    CAAAACAAGT GTCTTCCTGG AGATCCGAAT TACCTTGTTG GAGCCAACTG TGTTTCTGTC
    CTGATTGACC ACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011249145.1
    CDS115..5652
    Translation

    Target ORF information:

    RefSeq Version XM_011250843.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus tight junction protein 1 (Tjp1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011250843.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT
    CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA
    CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC
    AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA
    CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA
    AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT
    GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG
    AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG
    TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC
    ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT
    GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT
    GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA
    CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC
    TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT
    GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA
    CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG
    CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT
    AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG
    GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT
    GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT
    CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC
    CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT
    CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT
    GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT
    ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG
    TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA
    ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA
    ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT
    ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC
    ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT
    GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC
    CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT
    CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT
    CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA
    GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT
    AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG
    ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA
    ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT
    CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCCTCATCT
    CCAGTCCCTT ACCTTTCGCC TGAAACAACC CCAGCATCAT CAGCCTCTGC AGTTAATCAT
    AATGTCAGTG TAACTAATGT CAGCCTGGAG GAGCCTGCCC CAGCCCCTCC CACCTCGCAC
    GCATCACAGC CTGGTTGTTT AGGAGCACCA AGTGCTGAGG CAGCTCACGT AGTGCTCAGA
    GGTGAAGGAC CACCATTGCC GCCGCATGCA GACCCAGCAA AGGTGTACAG GAAGGAGCCG
    TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA
    GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA
    AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA
    GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT
    GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT
    CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT
    TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG
    CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC
    AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC
    AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC
    TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT
    TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA
    ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC
    AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT
    GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT
    AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG
    CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT
    GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG
    AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT
    TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAGG GAAAACCCGA AACTGATGCT
    GTGGATAGAT CATTCAGTGA GAAACGTTAT GATCCAGCCC AGGCCACGCC TCCTCCTCCT
    CCGTTGCCCT CACAGTACAG CCAGCCAGCT CCACCTCTGT CCAGCTCTTC TCTCCACATA
    CATTCCAAGG GCGCCCAGGG TGAAGGCAAC TCAGTATCAT TGGATTTTCA GAACTCATAT
    ATGTCCAAAC CAGACCCACC CCCATCTCAG AGTAAACCAG CAACTTTCAG ACCACCAACT
    CGAGAAGACC CCCCTCAGAC TTTCTATCCG CAGAAAAGTT TCCCAGACAA AGCTCCAGTT
    AACGGAGCTG AGCAGACTCA GAAAACCATC ACTCCGGTGT ACAATCGATT CACACCAAAG
    CCGTACACAA GTTCTGCCCG GCCATTTGAA CGCAAATTTG AAAGTCCGAA GTTCAACCAT
    AATCTTCTGC CAAGTGAAAC TGTACATAAA CCTGAATTGT CTTCAAAAAC TCCCACTTCC
    CCAAAAACTC TTATGAAAGC TCATAGTTCA ACACAGCCTC CAGAGTTTGA CAGTGGAGTT
    GAAACTTTCT CTGTTCACAC AGATAAGCCT AAATATCAAA TGAATAATAT CAGCACCATG
    CCTAAAGCTG TCCCTGTGAG TCCTTCAGCT GTGGAAGAAG ATGAAGATGA GGATGGTCAT
    ACTGTAGTGG CTACAGCTCG TGGCATTTTT AACAGCAATG GTGGTGTGTT GAGTTCCATA
    GAAACCGGTG TTAGTATAAT TATCCCACAA GGAGCCATTC CTGAAGGAAT TGAGCAAGAA
    ATCTATTTCA AAGTCTGCAG AGACAATAGC ATTCTCCCAC CTTTAGATAA AGAGAAAGGT
    GAAACTCTGC TGAGCCCCCT AGTGATGTGT GGGCCCCATG GCCTCAAGTT CCTGAAGCCC
    GTGGAGCTAC GCTTGCCACA CTGTGCGTCC ATGACTCCTG ACGGTGACCC TAAAACCTGG
    CAAAACAAGT GTCTTCCTGG AGATCCGAAT TACCTTGTTG GAGCCAACTG TGTTTCTGTC
    CTGATTGACC ACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu89424
    Clone ID Related Accession (Same CDS sequence) XM_030242356.1
    Accession Version XM_030242356.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5313bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product tight junction protein ZO-1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGAGGAAA CAGCTATATG GGAACAGCAC ACAGTGACGC TTCACAGGGC TCCTGGGTTT 
    GGATTTGGAA TTGCAATCTC TGGTGGAAGA GATAATCCTC ATTTTCAGAG TGGGGAAACC
    TCCATAGTGA TTTCTGATGT GTTAAAAGGA GGGCCAGCTG AAGGACAGCT ACAGGAAAAT
    GACCGAGTTG CAATGGTTAA CGGAGTTTCA ATGGATAACG TTGAACATGC TTTTGCTGTT
    CAGCAGCTAA GGAAGAGTGG GAAAAACGCA AAAATTACTA TCCGAAGGAA GAAGAAAGTT
    CAGATCCCTG TAAGTCACCC AGATCCTGAG CCGGTGTCTG ATAATGAAGA CGATAGTTAT
    GACGAAGAAG TGCATGACCC AAGAGCTGGC CGCGGTGCTT TAGCGAACAG AAGGAGCGAG
    AAGAGCTGGG CAAGGGATAG GAGTGCAAGC AGGGAGAGGA GCCTGTCCCC TCGCTCGGAC
    AGGCGGTCCG TGGCCTCCAG TCAGCCCGCA AAGCCCACCA AGGTCACACT GGTGAAGTCT
    CGGAAAAATG AAGAATATGG TCTTCGATTG GCCAGCCATA TATTTGTAAA GGAAATTTCA
    CAAGATAGTT TGGCAGCAAG AGATGGTAAC ATTCAAGAAG GGGATGTTGT CTTGAAGATA
    AATGGTACTG TGACAGAAAA TATGTCATTG ACAGATGCAA AAACACTGAT AGAAAGGTCT
    AAAGGCAAGT TAAAAATGGT AGTGCAAAGA GATGAGCGGG CTACCTTACT GAACGTCCCT
    GACCTTTCGG ATAGTATCCA TTCTGCTAAT GCCTCGGAAA GAGATGACAT TTCAGAAATT
    CAGTCACTAG CATCAGACCA TTCAGGTCGC TCGCATGACA GGCCACCCCG CCGCAGCCAG
    TCACGATCTC CTGACCAACG TTCAGAGCCC TCCGATCATT CCACGCAGTC TCCACAGCAG
    CCCAGCAATG GCAGTCTCCG GAGCAGAGAG GAAGAGCGAA TGTCTAAACC TGGGGCCATC
    TCAACTCCTG TAAAACATGT AGACGATCAT CCACCCAAAG CAGTGGAAGA AGTTACAGTT
    GAGAAAAATG AGAAGCAGAC GCCCACTCTT CCAGAACCGA AACCTGTGTA TGCTCAAGTT
    GGACAACCAG ATGTGGATTT ACCCGTCAGC CCTTCTGATG GTGCTCTGCC TAATTCAGCT
    CATGAAGACG GGATACTTAG GCCCAGCATG AAACTGGTAA AATTCAGAAA AGGAGATAGT
    GTGGGTTTGC GACTAGCTGG TGGAAATGAT GTCGGAATAT TTGTAGCTGG CGTTCTAGAA
    GATAGCCCTG CAGCCAAAGA AGGCTTAGAG GAAGGTGATC AAATTCTCAG GGTGAACAAT
    GTAGATTTCA CAAATATCAT AAGGGAAGAG GCCGTCCTTT TCCTCCTTGA CCTCCCTAAA
    GGTGAAGAAG TGACCATACT GGCTCAGAAG AAGAAGGACG TTTATCGCCG CATTGTAGAA
    TCAGATGTAG GAGATTCATT CTATATTAGA ACGCATTTTG AATATGAAAA AGAATCTCCT
    TACGGACTTA GTTTTAACAA AGGAGAGGTG TTCCGGGTCG TGGATACTTT ATACAATGGA
    AAGCTGGGCT CTTGGCTTGC CATTCGAATT GGCAAAAATC ATAAGGAGGT AGAACGAGGC
    ATCATCCCTA ATAAGAACAG AGCTGAACAG TTAGCCAGTG TACAGTACAC ACTTCCAAAG
    ACAGCGGGTG GTGATCGGGC AGACTTCTGG AGGTTTCGAG GTCTTCGCAG CTCCAAGAGA
    AATCTTCGAA AAAGCAGAGA GGACTTGTCA GCTCAGCCAG TTCAAACTAA GTTCCCAGCT
    TATGAAAGGG TTGTTCTTCG AGAAGCTGGA TTCCTAAGAC CTGTAACCAT CTTTGGACCA
    ATAGCTGATG TTGCCAGAGA AAAGTTGGCA AGAGAGGAGC CAGATATCTA TCAGATTGCA
    AAAAGTGAAC CACGAGATGC TGGGACTGAC CATCGTAGCT CTGGCATCAT TCGCCTTCAT
    ACAATAAAGC AAATCATAGA TCAGGATAAA CATGCTTTAT TAGATGTAAC GCCAAATGCG
    GTTGATCGTC TTAATTATGC GCAGTGGTAT CCAATTGTTG TGTTCCTTAA CCCTGACTCT
    AAGCAAGGTG TAAAAACAAT GAGGATGAGG TTGTGTCCGG AGTCTCGGAA AAGCGCCAGG
    AAGCTATATG AACGCTCTCA TAAGCTTCGT AAGAACAATC ACCATCTCTT CACAACTACA
    ATTAACTTAA ACTCAATGAA TGATGGTTGG TACGGTGCCC TGAAAGAAGC GATTCAGCAG
    CAACAGAACC AGCTGGTGTG GGTCTCTGAG GGGAAGGCGG ATGGTGCTAC AAGTGATGAC
    CTTGATTTGC ATGACGATCG TCTGTCCTAC CTGTCAGCCC CAGGTAGTGA GTACTCAATG
    TATAGCACGG ACAGTAGACA CACTTCTGAC TATGAAGACA CAGATACAGA AGGCGGGGCC
    TACACTGATC AAGAACTAGA TGAAACTCTT AATGATGAGG TGGGGACTCC CCCGGAGTCT
    GCCATTACAC GGTCCTCTGA GCCTGTAAGA GAGGATTCCT CTGGAATGCA TCATGAAAAC
    CAGACATACC CTCCTTACTC ACCACAAGCG CAGCCACAAG CTATTCATAG AATAGACTCC
    CCTGGACTTA AGCCAGCCTC TCAACAGAAA GCAGAAGCCT CATCTCCAGT CCCTTACCTT
    TCGCCTGAAA CAACCCCAGC ATCATCAGCC TCTGCAGTTA ATCATAATGT CAGTGTAACT
    AATGTCAGCC TGGAGGAGCC TGCCCCAGCC CCTCCCACCT CGCACGCATC ACAGCCTGGT
    TGTTTAGGAG CACCAAGTGC TGAGGCAGCT CACGTAGTGC TCAGAGGTGA AGGACCACCA
    TTGCCGCCGC ATGCAGACCC AGCAAAGGTG TACAGGAAGG AGCCGTATTC TGAAGAAATG
    ATGAGACAAA ACCATATTTT AAAACAACCA GCTCTTGGTC ACCCAGGGCA GAGGCCAGAT
    AAAGAGCCAA ATCTAGCCTA TGAACCCCAA CTTCCATATA TAGAAAAACA AGCCAGCAGA
    GACCTTGAGC AGCCGTCATA CAGGTATGAG GTCTCAAGCT ACACAGACCA GTTTTCTCGG
    AACTATGACC ATCGCCTACG GTTTGAAGAT CGAATCCCTA CCTATGAAGA CCAGTGGTCA
    TATTATGATG ACAAACAGCC CTACCAACCT CGGCCTTTTG AGAATCAGCA TCCCCGAGAC
    CTGGACTCCA GACAACATCC CGAAGAGGCT TCAGAACGAG GTTATTTCCA GCGTTTTGAA
    GAGCCAGCCC CTCTGTCGTA CGACAGTAGA ACACGCTATG AGCAGCTGCC TCGAACCTCT
    ACTCTACGAC ATGAAGAGCA GCCAGCCCCT GCATATGAGG TGCACAACAG GTACAGGCCA
    GAGGCACAGC CCTATTCTTC AACAGGCCCT AAGTCATCTG AGCCCAAGCA GTACTTTGAC
    CAGTACCCGC GAAGTTATGA GCAAGTTCCA CCACCAGGCT TTACCTCCAA AACAGGCCAT
    TACGAGCCTC TCCATGGTGC TGCAGTTGTC CCTCCTCTGA TACCTTCCTC TCAACAAAAG
    CCAGAAGTCC TGCCCTCGGC TACCAAACCA CAGCCTCCAC CCCCAACCCT AACTGAGGAG
    GAGGAGGATC CAGCAATGAA ACCACAGTCT GTGCTCACCA GAGTCAAAAT GTTTGAAAAC
    AAAAGATCTG CGTCTTTGGA GAACAAGAAA GATGTGAATG ACACTGCCAG CTTTAAGCCT
    CCGGAAGTAG CATCTAAACC TCCAGGTGCT TCTCTTGCTG GCCCTAAACC TGTCCCTCAG
    AGTCAGTTTA GTGAGCACGA CAAAACGCTC TACAGGCTCC CAGAGCCTCA GAAACCTCAA
    GTGAAGCCAC CCGAAGATAT TGTTCGATCA AATCATTACG ACCCTGAAGA GGATGAAGAA
    TATTACCGGA AACAGCTCTC CTACTTTGAC CGAAGAAGTT TTGAGAGCAA GCCTTCTGCA
    CATCTTCCTG CTGGCCATCA CTCAGAGCCT GCTAAGCCAG TCCATTCTCA GAGCCAGCCC
    AACTTCTCTA GTTACTCTTC AAAATTTCTT GGCTCTTACA CTAGCTATGA CTACTTGAAA
    AGGAACATCA AGTGGGGAAA ACCCGAAACT GATGCTGTGG ATAGATCATT CAGTGAGAAA
    CGTTATGATC CAGCCCAGGC CACGCCTCCT CCTCCTCCGT TGCCCTCACA GTACAGCCAG
    CCAGCTCCAC CTCTGTCCAG CTCTTCTCTC CACATACATT CCAAGGGCGC CCAGGGTGAA
    GGCAACTCAG TATCATTGGA TTTTCAGAAC TCATATATGT CCAAACCAGA CCCACCCCCA
    TCTCAGAGTA AACCAGCAAC TTTCAGACCA CCAACTCGAG AAGACCCCCC TCAGACTTTC
    TATCCGCAGA AAAGTTTCCC AGACAAAGCT CCAGTTAACG GAGCTGAGCA GACTCAGAAA
    ACCATCACTC CGGTGTACAA TCGATTCACA CCAAAGCCGT ACACAAGTTC TGCCCGGCCA
    TTTGAACGCA AATTTGAAAG TCCGAAGTTC AACCATAATC TTCTGCCAAG TGAAACTGTA
    CATAAACCTG AATTGTCTTC AAAAACTCCC ACTTCCCCAA AAACTCTTAT GAAAGCTCAT
    AGTTCAACAC AGCCTCCAGA GTTTGACAGT GGAGTTGAAA CTTTCTCTGT TCACACAGAT
    AAGCCTAAAT ATCAAATGAA TAATATCAGC ACCATGCCTA AAGCTGTCCC TGTGAGTCCT
    TCAGCTGTGG AAGAAGATGA AGATGAGGAT GGTCATACTG TAGTGGCTAC AGCTCGTGGC
    ATTTTTAACA GCAATGGTGG TGTGTTGAGT TCCATAGAAA CCGGTGTTAG TATAATTATC
    CCACAAGGAG CCATTCCTGA AGGAATTGAG CAAGAAATCT ATTTCAAAGT CTGCAGAGAC
    AATAGCATTC TCCCACCTTT AGATAAAGAG AAAGGTGAAA CTCTGCTGAG CCCCCTAGTG
    ATGTGTGGGC CCCATGGCCT CAAGTTCCTG AAGCCCGTGG AGCTACGCTT GCCACACTGT
    GCGTCCATGA CTCCTGACGG TTGGTCTTTT GCTCTAAAAT CATCCGACTC CTCGTCGGGT
    GACCCTAAAA CCTGGCAAAA CAAGTGTCTT CCTGGAGATC CGAATTACCT TGTTGGAGCC
    AACTGTGTTT CTGTCCTGAT TGACCACTTT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030098216.1
    CDS743..6055
    Translation

    Target ORF information:

    RefSeq Version XM_030242356.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus tight junction protein 1 (Tjp1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030242356.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGAGGAAA CAGCTATATG GGAACAGCAC ACAGTGACGC TTCACAGGGC TCCTGGGTTT 
    GGATTTGGAA TTGCAATCTC TGGTGGAAGA GATAATCCTC ATTTTCAGAG TGGGGAAACC
    TCCATAGTGA TTTCTGATGT GTTAAAAGGA GGGCCAGCTG AAGGACAGCT ACAGGAAAAT
    GACCGAGTTG CAATGGTTAA CGGAGTTTCA ATGGATAACG TTGAACATGC TTTTGCTGTT
    CAGCAGCTAA GGAAGAGTGG GAAAAACGCA AAAATTACTA TCCGAAGGAA GAAGAAAGTT
    CAGATCCCTG TAAGTCACCC AGATCCTGAG CCGGTGTCTG ATAATGAAGA CGATAGTTAT
    GACGAAGAAG TGCATGACCC AAGAGCTGGC CGCGGTGCTT TAGCGAACAG AAGGAGCGAG
    AAGAGCTGGG CAAGGGATAG GAGTGCAAGC AGGGAGAGGA GCCTGTCCCC TCGCTCGGAC
    AGGCGGTCCG TGGCCTCCAG TCAGCCCGCA AAGCCCACCA AGGTCACACT GGTGAAGTCT
    CGGAAAAATG AAGAATATGG TCTTCGATTG GCCAGCCATA TATTTGTAAA GGAAATTTCA
    CAAGATAGTT TGGCAGCAAG AGATGGTAAC ATTCAAGAAG GGGATGTTGT CTTGAAGATA
    AATGGTACTG TGACAGAAAA TATGTCATTG ACAGATGCAA AAACACTGAT AGAAAGGTCT
    AAAGGCAAGT TAAAAATGGT AGTGCAAAGA GATGAGCGGG CTACCTTACT GAACGTCCCT
    GACCTTTCGG ATAGTATCCA TTCTGCTAAT GCCTCGGAAA GAGATGACAT TTCAGAAATT
    CAGTCACTAG CATCAGACCA TTCAGGTCGC TCGCATGACA GGCCACCCCG CCGCAGCCAG
    TCACGATCTC CTGACCAACG TTCAGAGCCC TCCGATCATT CCACGCAGTC TCCACAGCAG
    CCCAGCAATG GCAGTCTCCG GAGCAGAGAG GAAGAGCGAA TGTCTAAACC TGGGGCCATC
    TCAACTCCTG TAAAACATGT AGACGATCAT CCACCCAAAG CAGTGGAAGA AGTTACAGTT
    GAGAAAAATG AGAAGCAGAC GCCCACTCTT CCAGAACCGA AACCTGTGTA TGCTCAAGTT
    GGACAACCAG ATGTGGATTT ACCCGTCAGC CCTTCTGATG GTGCTCTGCC TAATTCAGCT
    CATGAAGACG GGATACTTAG GCCCAGCATG AAACTGGTAA AATTCAGAAA AGGAGATAGT
    GTGGGTTTGC GACTAGCTGG TGGAAATGAT GTCGGAATAT TTGTAGCTGG CGTTCTAGAA
    GATAGCCCTG CAGCCAAAGA AGGCTTAGAG GAAGGTGATC AAATTCTCAG GGTGAACAAT
    GTAGATTTCA CAAATATCAT AAGGGAAGAG GCCGTCCTTT TCCTCCTTGA CCTCCCTAAA
    GGTGAAGAAG TGACCATACT GGCTCAGAAG AAGAAGGACG TTTATCGCCG CATTGTAGAA
    TCAGATGTAG GAGATTCATT CTATATTAGA ACGCATTTTG AATATGAAAA AGAATCTCCT
    TACGGACTTA GTTTTAACAA AGGAGAGGTG TTCCGGGTCG TGGATACTTT ATACAATGGA
    AAGCTGGGCT CTTGGCTTGC CATTCGAATT GGCAAAAATC ATAAGGAGGT AGAACGAGGC
    ATCATCCCTA ATAAGAACAG AGCTGAACAG TTAGCCAGTG TACAGTACAC ACTTCCAAAG
    ACAGCGGGTG GTGATCGGGC AGACTTCTGG AGGTTTCGAG GTCTTCGCAG CTCCAAGAGA
    AATCTTCGAA AAAGCAGAGA GGACTTGTCA GCTCAGCCAG TTCAAACTAA GTTCCCAGCT
    TATGAAAGGG TTGTTCTTCG AGAAGCTGGA TTCCTAAGAC CTGTAACCAT CTTTGGACCA
    ATAGCTGATG TTGCCAGAGA AAAGTTGGCA AGAGAGGAGC CAGATATCTA TCAGATTGCA
    AAAAGTGAAC CACGAGATGC TGGGACTGAC CATCGTAGCT CTGGCATCAT TCGCCTTCAT
    ACAATAAAGC AAATCATAGA TCAGGATAAA CATGCTTTAT TAGATGTAAC GCCAAATGCG
    GTTGATCGTC TTAATTATGC GCAGTGGTAT CCAATTGTTG TGTTCCTTAA CCCTGACTCT
    AAGCAAGGTG TAAAAACAAT GAGGATGAGG TTGTGTCCGG AGTCTCGGAA AAGCGCCAGG
    AAGCTATATG AACGCTCTCA TAAGCTTCGT AAGAACAATC ACCATCTCTT CACAACTACA
    ATTAACTTAA ACTCAATGAA TGATGGTTGG TACGGTGCCC TGAAAGAAGC GATTCAGCAG
    CAACAGAACC AGCTGGTGTG GGTCTCTGAG GGGAAGGCGG ATGGTGCTAC AAGTGATGAC
    CTTGATTTGC ATGACGATCG TCTGTCCTAC CTGTCAGCCC CAGGTAGTGA GTACTCAATG
    TATAGCACGG ACAGTAGACA CACTTCTGAC TATGAAGACA CAGATACAGA AGGCGGGGCC
    TACACTGATC AAGAACTAGA TGAAACTCTT AATGATGAGG TGGGGACTCC CCCGGAGTCT
    GCCATTACAC GGTCCTCTGA GCCTGTAAGA GAGGATTCCT CTGGAATGCA TCATGAAAAC
    CAGACATACC CTCCTTACTC ACCACAAGCG CAGCCACAAG CTATTCATAG AATAGACTCC
    CCTGGACTTA AGCCAGCCTC TCAACAGAAA GCAGAAGCCT CATCTCCAGT CCCTTACCTT
    TCGCCTGAAA CAACCCCAGC ATCATCAGCC TCTGCAGTTA ATCATAATGT CAGTGTAACT
    AATGTCAGCC TGGAGGAGCC TGCCCCAGCC CCTCCCACCT CGCACGCATC ACAGCCTGGT
    TGTTTAGGAG CACCAAGTGC TGAGGCAGCT CACGTAGTGC TCAGAGGTGA AGGACCACCA
    TTGCCGCCGC ATGCAGACCC AGCAAAGGTG TACAGGAAGG AGCCGTATTC TGAAGAAATG
    ATGAGACAAA ACCATATTTT AAAACAACCA GCTCTTGGTC ACCCAGGGCA GAGGCCAGAT
    AAAGAGCCAA ATCTAGCCTA TGAACCCCAA CTTCCATATA TAGAAAAACA AGCCAGCAGA
    GACCTTGAGC AGCCGTCATA CAGGTATGAG GTCTCAAGCT ACACAGACCA GTTTTCTCGG
    AACTATGACC ATCGCCTACG GTTTGAAGAT CGAATCCCTA CCTATGAAGA CCAGTGGTCA
    TATTATGATG ACAAACAGCC CTACCAACCT CGGCCTTTTG AGAATCAGCA TCCCCGAGAC
    CTGGACTCCA GACAACATCC CGAAGAGGCT TCAGAACGAG GTTATTTCCA GCGTTTTGAA
    GAGCCAGCCC CTCTGTCGTA CGACAGTAGA ACACGCTATG AGCAGCTGCC TCGAACCTCT
    ACTCTACGAC ATGAAGAGCA GCCAGCCCCT GCATATGAGG TGCACAACAG GTACAGGCCA
    GAGGCACAGC CCTATTCTTC AACAGGCCCT AAGTCATCTG AGCCCAAGCA GTACTTTGAC
    CAGTACCCGC GAAGTTATGA GCAAGTTCCA CCACCAGGCT TTACCTCCAA AACAGGCCAT
    TACGAGCCTC TCCATGGTGC TGCAGTTGTC CCTCCTCTGA TACCTTCCTC TCAACAAAAG
    CCAGAAGTCC TGCCCTCGGC TACCAAACCA CAGCCTCCAC CCCCAACCCT AACTGAGGAG
    GAGGAGGATC CAGCAATGAA ACCACAGTCT GTGCTCACCA GAGTCAAAAT GTTTGAAAAC
    AAAAGATCTG CGTCTTTGGA GAACAAGAAA GATGTGAATG ACACTGCCAG CTTTAAGCCT
    CCGGAAGTAG CATCTAAACC TCCAGGTGCT TCTCTTGCTG GCCCTAAACC TGTCCCTCAG
    AGTCAGTTTA GTGAGCACGA CAAAACGCTC TACAGGCTCC CAGAGCCTCA GAAACCTCAA
    GTGAAGCCAC CCGAAGATAT TGTTCGATCA AATCATTACG ACCCTGAAGA GGATGAAGAA
    TATTACCGGA AACAGCTCTC CTACTTTGAC CGAAGAAGTT TTGAGAGCAA GCCTTCTGCA
    CATCTTCCTG CTGGCCATCA CTCAGAGCCT GCTAAGCCAG TCCATTCTCA GAGCCAGCCC
    AACTTCTCTA GTTACTCTTC AAAATTTCTT GGCTCTTACA CTAGCTATGA CTACTTGAAA
    AGGAACATCA AGTGGGGAAA ACCCGAAACT GATGCTGTGG ATAGATCATT CAGTGAGAAA
    CGTTATGATC CAGCCCAGGC CACGCCTCCT CCTCCTCCGT TGCCCTCACA GTACAGCCAG
    CCAGCTCCAC CTCTGTCCAG CTCTTCTCTC CACATACATT CCAAGGGCGC CCAGGGTGAA
    GGCAACTCAG TATCATTGGA TTTTCAGAAC TCATATATGT CCAAACCAGA CCCACCCCCA
    TCTCAGAGTA AACCAGCAAC TTTCAGACCA CCAACTCGAG AAGACCCCCC TCAGACTTTC
    TATCCGCAGA AAAGTTTCCC AGACAAAGCT CCAGTTAACG GAGCTGAGCA GACTCAGAAA
    ACCATCACTC CGGTGTACAA TCGATTCACA CCAAAGCCGT ACACAAGTTC TGCCCGGCCA
    TTTGAACGCA AATTTGAAAG TCCGAAGTTC AACCATAATC TTCTGCCAAG TGAAACTGTA
    CATAAACCTG AATTGTCTTC AAAAACTCCC ACTTCCCCAA AAACTCTTAT GAAAGCTCAT
    AGTTCAACAC AGCCTCCAGA GTTTGACAGT GGAGTTGAAA CTTTCTCTGT TCACACAGAT
    AAGCCTAAAT ATCAAATGAA TAATATCAGC ACCATGCCTA AAGCTGTCCC TGTGAGTCCT
    TCAGCTGTGG AAGAAGATGA AGATGAGGAT GGTCATACTG TAGTGGCTAC AGCTCGTGGC
    ATTTTTAACA GCAATGGTGG TGTGTTGAGT TCCATAGAAA CCGGTGTTAG TATAATTATC
    CCACAAGGAG CCATTCCTGA AGGAATTGAG CAAGAAATCT ATTTCAAAGT CTGCAGAGAC
    AATAGCATTC TCCCACCTTT AGATAAAGAG AAAGGTGAAA CTCTGCTGAG CCCCCTAGTG
    ATGTGTGGGC CCCATGGCCT CAAGTTCCTG AAGCCCGTGG AGCTACGCTT GCCACACTGT
    GCGTCCATGA CTCCTGACGG TTGGTCTTTT GCTCTAAAAT CATCCGACTC CTCGTCGGGT
    GACCCTAAAA CCTGGCAAAA CAAGTGTCTT CCTGGAGATC CGAATTACCT TGTTGGAGCC
    AACTGTGTTT CTGTCCTGAT TGACCACTTT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu20010
    Clone ID Related Accession (Same CDS sequence) NM_009386.2
    Accession Version NM_009386.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5238bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-31
    Organism Mus musculus(house mouse)
    Product tight junction protein ZO-1 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC122222.6 and AC131741.4. On Jul 24, 2009 this sequence version replaced NM_009386.1. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCCGCCA GGGCCGCGGC CGCTAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
    CAGCACACAG TGACGCTTCA CAGGGCTCCT GGGTTTGGAT TTGGAATTGC AATCTCTGGT
    GGAAGAGATA ATCCTCATTT TCAGAGTGGG GAAACCTCCA TAGTGATTTC TGATGTGTTA
    AAAGGAGGGC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA
    GTTTCAATGG ATAACGTTGA ACATGCTTTT GCTGTTCAGC AGCTAAGGAA GAGTGGGAAA
    AACGCAAAAA TTACTATCCG AAGGAAGAAG AAAGTTCAGA TCCCTGTAAG TCACCCAGAT
    CCTGAGCCGG TGTCTGATAA TGAAGACGAT AGTTATGACG AAGAAGTGCA TGACCCAAGA
    GCTGGCCGCG GTGCTTTAGC GAACAGAAGG AGCGAGAAGA GCTGGGCAAG GGATAGGAGT
    GCAAGCAGGG AGAGGAGCCT GTCCCCTCGC TCGGACAGGC GGTCCGTGGC CTCCAGTCAG
    CCCGCAAAGC CCACCAAGGT CACACTGGTG AAGTCTCGGA AAAATGAAGA ATATGGTCTT
    CGATTGGCCA GCCATATATT TGTAAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT
    GGTAACATTC AAGAAGGGGA TGTTGTCTTG AAGATAAATG GTACTGTGAC AGAAAATATG
    TCATTGACAG ATGCAAAAAC ACTGATAGAA AGGTCTAAAG GCAAGTTAAA AATGGTAGTG
    CAAAGAGATG AGCGGGCTAC CTTACTGAAC GTCCCTGACC TTTCGGATAG TATCCATTCT
    GCTAATGCCT CGGAAAGAGA TGACATTTCA GAAATTCAGT CACTAGCATC AGACCATTCA
    GGTCGCTCGC ATGACAGGCC ACCCCGCCGC AGCCAGTCAC GATCTCCTGA CCAACGTTCA
    GAGCCCTCCG ATCATTCCAC GCAGTCTCCA CAGCAGCCCA GCAATGGCAG TCTCCGGAGC
    AGAGAGGAAG AGCGAATGTC TAAACCTGGG GCCATCTCAA CTCCTGTAAA ACATGTAGAC
    GATCATCCAC CCAAAGCAGT GGAAGAAGTT ACAGTTGAGA AAAATGAGAA GCAGACGCCC
    ACTCTTCCAG AACCGAAACC TGTGTATGCT CAAGTTGGAC AACCAGATGT GGATTTACCC
    GTCAGCCCTT CTGATGGTGC TCTGCCTAAT TCAGCTCATG AAGACGGGAT ACTTAGGCCC
    AGCATGAAAC TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGACT AGCTGGTGGA
    AATGATGTCG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAAGAAGGC
    TTAGAGGAAG GTGATCAAAT TCTCAGGGTG AACAATGTAG ATTTCACAAA TATCATAAGG
    GAAGAGGCCG TCCTTTTCCT CCTTGACCTC CCTAAAGGTG AAGAAGTGAC CATACTGGCT
    CAGAAGAAGA AGGACGTTTA TCGCCGCATT GTAGAATCAG ATGTAGGAGA TTCATTCTAT
    ATTAGAACGC ATTTTGAATA TGAAAAAGAA TCTCCTTACG GACTTAGTTT TAACAAAGGA
    GAGGTGTTCC GGGTCGTGGA TACTTTATAC AATGGAAAGC TGGGCTCTTG GCTTGCCATT
    CGAATTGGCA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT
    GAACAGTTAG CCAGTGTACA GTACACACTT CCAAAGACAG CGGGTGGTGA TCGGGCAGAC
    TTCTGGAGGT TTCGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAC
    TTGTCAGCTC AGCCAGTTCA AACTAAGTTC CCAGCTTATG AAAGGGTTGT TCTTCGAGAA
    GCTGGATTCC TAAGACCTGT AACCATCTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG
    TTGGCAAGAG AGGAGCCAGA TATCTATCAG ATTGCAAAAA GTGAACCACG AGATGCTGGG
    ACTGACCATC GTAGCTCTGG CATCATTCGC CTTCATACAA TAAAGCAAAT CATAGATCAG
    GATAAACATG CTTTATTAGA TGTAACGCCA AATGCGGTTG ATCGTCTTAA TTATGCGCAG
    TGGTATCCAA TTGTTGTGTT CCTTAACCCT GACTCTAAGC AAGGTGTAAA AACAATGAGG
    ATGAGGTTGT GTCCGGAGTC TCGGAAAAGC GCCAGGAAGC TATATGAACG CTCTCATAAG
    CTTCGTAAGA ACAATCACCA TCTCTTCACA ACTACAATTA ACTTAAACTC AATGAATGAT
    GGTTGGTACG GTGCCCTGAA AGAAGCGATT CAGCAGCAAC AGAACCAGCT GGTGTGGGTC
    TCTGAGGGGA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA CGATCGTCTG
    TCCTACCTGT CAGCCCCAGG TAGTGAGTAC TCAATGTATA GCACGGACAG TAGACACACT
    TCTGACTATG AAGACACAGA TACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA
    ACTCTTAATG ATGAGGTGGG GACTCCCCCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT
    GTAAGAGAGG ATTCCTCTGG AATGCATCAT GAAAACCAGA CATACCCTCC TTACTCACCA
    CAAGCGCAGC CACAAGCTAT TCATAGAATA GACTCCCCTG GACTTAAGCC AGCCTCTCAA
    CAGAAAGCAG AAGCCTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAC CCCAGCATCA
    TCAGCCTCTG CAGTTAATCA TAATGTCAGT GTAACTAATG TCAGCCTGGA GGAGCCTGCC
    CCAGCCCCTC CCACCTCGCA CGCATCACAG CCTGGTTGTT TAGGAGCACC AAGTGCTGAG
    GCAGCTCACG TAGTGCTCAG AGGTGAAGGA CCACCATTGC CGCCGCATGC AGACCCAGCA
    AAGGTGTACA GGAAGGAGCC GTATTCTGAA GAAATGATGA GACAAAACCA TATTTTAAAA
    CAACCAGCTC TTGGTCACCC AGGGCAGAGG CCAGATAAAG AGCCAAATCT AGCCTATGAA
    CCCCAACTTC CATATATAGA AAAACAAGCC AGCAGAGACC TTGAGCAGCC GTCATACAGG
    TATGAGGTCT CAAGCTACAC AGACCAGTTT TCTCGGAACT ATGACCATCG CCTACGGTTT
    GAAGATCGAA TCCCTACCTA TGAAGACCAG TGGTCATATT ATGATGACAA ACAGCCCTAC
    CAACCTCGGC CTTTTGAGAA TCAGCATCCC CGAGACCTGG ACTCCAGACA ACATCCCGAA
    GAGGCTTCAG AACGAGGTTA TTTCCAGCGT TTTGAAGAGC CAGCCCCTCT GTCGTACGAC
    AGTAGAACAC GCTATGAGCA GCTGCCTCGA ACCTCTACTC TACGACATGA AGAGCAGCCA
    GCCCCTGCAT ATGAGGTGCA CAACAGGTAC AGGCCAGAGG CACAGCCCTA TTCTTCAACA
    GGCCCTAAGT CATCTGAGCC CAAGCAGTAC TTTGACCAGT ACCCGCGAAG TTATGAGCAA
    GTTCCACCAC CAGGCTTTAC CTCCAAAACA GGCCATTACG AGCCTCTCCA TGGTGCTGCA
    GTTGTCCCTC CTCTGATACC TTCCTCTCAA CAAAAGCCAG AAGTCCTGCC CTCGGCTACC
    AAACCACAGC CTCCACCCCC AACCCTAACT GAGGAGGAGG AGGATCCAGC AATGAAACCA
    CAGTCTGTGC TCACCAGAGT CAAAATGTTT GAAAACAAAA GATCTGCGTC TTTGGAGAAC
    AAGAAAGATG TGAATGACAC TGCCAGCTTT AAGCCTCCGG AAGTAGCATC TAAACCTCCA
    GGTGCTTCTC TTGCTGGCCC TAAACCTGTC CCTCAGAGTC AGTTTAGTGA GCACGACAAA
    ACGCTCTACA GGCTCCCAGA GCCTCAGAAA CCTCAAGTGA AGCCACCCGA AGATATTGTT
    CGATCAAATC ATTACGACCC TGAAGAGGAT GAAGAATATT ACCGGAAACA GCTCTCCTAC
    TTTGACCGAA GAAGTTTTGA GAGCAAGCCT TCTGCACATC TTCCTGCTGG CCATCACTCA
    GAGCCTGCTA AGCCAGTCCA TTCTCAGAGC CAGCCCAACT TCTCTAGTTA CTCTTCAAAG
    GGAAAACCCG AAACTGATGC TGTGGATAGA TCATTCAGTG AGAAACGTTA TGATCCAGCC
    CAGGCCACGC CTCCTCCTCC TCCGTTGCCC TCACAGTACA GCCAGCCAGC TCCACCTCTG
    TCCAGCTCTT CTCTCCACAT ACATTCCAAG GGCGCCCAGG GTGAAGGCAA CTCAGTATCA
    TTGGATTTTC AGAACTCATA TATGTCCAAA CCAGACCCAC CCCCATCTCA GAGTAAACCA
    GCAACTTTCA GACCACCAAC TCGAGAAGAC CCCCCTCAGA CTTTCTATCC GCAGAAAAGT
    TTCCCAGACA AAGCTCCAGT TAACGGAGCT GAGCAGACTC AGAAAACCAT CACTCCGGTG
    TACAATCGAT TCACACCAAA GCCGTACACA AGTTCTGCCC GGCCATTTGA ACGCAAATTT
    GAAAGTCCGA AGTTCAACCA TAATCTTCTG CCAAGTGAAA CTGTACATAA ACCTGAATTG
    TCTTCAAAAA CTCCCACTTC CCCAAAAACT CTTATGAAAG CTCATAGTTC AACACAGCCT
    CCAGAGTTTG ACAGTGGAGT TGAAACTTTC TCTGTTCACA CAGATAAGCC TAAATATCAA
    ATGAATAATA TCAGCACCAT GCCTAAAGCT GTCCCTGTGA GTCCTTCAGC TGTGGAAGAA
    GATGAAGATG AGGATGGTCA TACTGTAGTG GCTACAGCTC GTGGCATTTT TAACAGCAAT
    GGTGGTGTGT TGAGTTCCAT AGAAACCGGT GTTAGTATAA TTATCCCACA AGGAGCCATT
    CCTGAAGGAA TTGAGCAAGA AATCTATTTC AAAGTCTGCA GAGACAATAG CATTCTCCCA
    CCTTTAGATA AAGAGAAAGG TGAAACTCTG CTGAGCCCCC TAGTGATGTG TGGGCCCCAT
    GGCCTCAAGT TCCTGAAGCC CGTGGAGCTA CGCTTGCCAC ACTGTGACCC TAAAACCTGG
    CAAAACAAGT GTCTTCCTGG AGATCCGAAT TACCTTGTTG GAGCCAACTG TGTTTCTGTC
    CTGATTGACC ACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_033412.2
    CDS409..5646
    Translation

    Target ORF information:

    RefSeq Version NM_009386.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus tight junction protein 1 (Tjp1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_009386.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCCGCCA GGGCCGCGGC CGCTAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
    CAGCACACAG TGACGCTTCA CAGGGCTCCT GGGTTTGGAT TTGGAATTGC AATCTCTGGT
    GGAAGAGATA ATCCTCATTT TCAGAGTGGG GAAACCTCCA TAGTGATTTC TGATGTGTTA
    AAAGGAGGGC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA
    GTTTCAATGG ATAACGTTGA ACATGCTTTT GCTGTTCAGC AGCTAAGGAA GAGTGGGAAA
    AACGCAAAAA TTACTATCCG AAGGAAGAAG AAAGTTCAGA TCCCTGTAAG TCACCCAGAT
    CCTGAGCCGG TGTCTGATAA TGAAGACGAT AGTTATGACG AAGAAGTGCA TGACCCAAGA
    GCTGGCCGCG GTGCTTTAGC GAACAGAAGG AGCGAGAAGA GCTGGGCAAG GGATAGGAGT
    GCAAGCAGGG AGAGGAGCCT GTCCCCTCGC TCGGACAGGC GGTCCGTGGC CTCCAGTCAG
    CCCGCAAAGC CCACCAAGGT CACACTGGTG AAGTCTCGGA AAAATGAAGA ATATGGTCTT
    CGATTGGCCA GCCATATATT TGTAAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT
    GGTAACATTC AAGAAGGGGA TGTTGTCTTG AAGATAAATG GTACTGTGAC AGAAAATATG
    TCATTGACAG ATGCAAAAAC ACTGATAGAA AGGTCTAAAG GCAAGTTAAA AATGGTAGTG
    CAAAGAGATG AGCGGGCTAC CTTACTGAAC GTCCCTGACC TTTCGGATAG TATCCATTCT
    GCTAATGCCT CGGAAAGAGA TGACATTTCA GAAATTCAGT CACTAGCATC AGACCATTCA
    GGTCGCTCGC ATGACAGGCC ACCCCGCCGC AGCCAGTCAC GATCTCCTGA CCAACGTTCA
    GAGCCCTCCG ATCATTCCAC GCAGTCTCCA CAGCAGCCCA GCAATGGCAG TCTCCGGAGC
    AGAGAGGAAG AGCGAATGTC TAAACCTGGG GCCATCTCAA CTCCTGTAAA ACATGTAGAC
    GATCATCCAC CCAAAGCAGT GGAAGAAGTT ACAGTTGAGA AAAATGAGAA GCAGACGCCC
    ACTCTTCCAG AACCGAAACC TGTGTATGCT CAAGTTGGAC AACCAGATGT GGATTTACCC
    GTCAGCCCTT CTGATGGTGC TCTGCCTAAT TCAGCTCATG AAGACGGGAT ACTTAGGCCC
    AGCATGAAAC TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGACT AGCTGGTGGA
    AATGATGTCG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAAGAAGGC
    TTAGAGGAAG GTGATCAAAT TCTCAGGGTG AACAATGTAG ATTTCACAAA TATCATAAGG
    GAAGAGGCCG TCCTTTTCCT CCTTGACCTC CCTAAAGGTG AAGAAGTGAC CATACTGGCT
    CAGAAGAAGA AGGACGTTTA TCGCCGCATT GTAGAATCAG ATGTAGGAGA TTCATTCTAT
    ATTAGAACGC ATTTTGAATA TGAAAAAGAA TCTCCTTACG GACTTAGTTT TAACAAAGGA
    GAGGTGTTCC GGGTCGTGGA TACTTTATAC AATGGAAAGC TGGGCTCTTG GCTTGCCATT
    CGAATTGGCA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT
    GAACAGTTAG CCAGTGTACA GTACACACTT CCAAAGACAG CGGGTGGTGA TCGGGCAGAC
    TTCTGGAGGT TTCGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAC
    TTGTCAGCTC AGCCAGTTCA AACTAAGTTC CCAGCTTATG AAAGGGTTGT TCTTCGAGAA
    GCTGGATTCC TAAGACCTGT AACCATCTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG
    TTGGCAAGAG AGGAGCCAGA TATCTATCAG ATTGCAAAAA GTGAACCACG AGATGCTGGG
    ACTGACCATC GTAGCTCTGG CATCATTCGC CTTCATACAA TAAAGCAAAT CATAGATCAG
    GATAAACATG CTTTATTAGA TGTAACGCCA AATGCGGTTG ATCGTCTTAA TTATGCGCAG
    TGGTATCCAA TTGTTGTGTT CCTTAACCCT GACTCTAAGC AAGGTGTAAA AACAATGAGG
    ATGAGGTTGT GTCCGGAGTC TCGGAAAAGC GCCAGGAAGC TATATGAACG CTCTCATAAG
    CTTCGTAAGA ACAATCACCA TCTCTTCACA ACTACAATTA ACTTAAACTC AATGAATGAT
    GGTTGGTACG GTGCCCTGAA AGAAGCGATT CAGCAGCAAC AGAACCAGCT GGTGTGGGTC
    TCTGAGGGGA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA CGATCGTCTG
    TCCTACCTGT CAGCCCCAGG TAGTGAGTAC TCAATGTATA GCACGGACAG TAGACACACT
    TCTGACTATG AAGACACAGA TACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA
    ACTCTTAATG ATGAGGTGGG GACTCCCCCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT
    GTAAGAGAGG ATTCCTCTGG AATGCATCAT GAAAACCAGA CATACCCTCC TTACTCACCA
    CAAGCGCAGC CACAAGCTAT TCATAGAATA GACTCCCCTG GACTTAAGCC AGCCTCTCAA
    CAGAAAGCAG AAGCCTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAC CCCAGCATCA
    TCAGCCTCTG CAGTTAATCA TAATGTCAGT GTAACTAATG TCAGCCTGGA GGAGCCTGCC
    CCAGCCCCTC CCACCTCGCA CGCATCACAG CCTGGTTGTT TAGGAGCACC AAGTGCTGAG
    GCAGCTCACG TAGTGCTCAG AGGTGAAGGA CCACCATTGC CGCCGCATGC AGACCCAGCA
    AAGGTGTACA GGAAGGAGCC GTATTCTGAA GAAATGATGA GACAAAACCA TATTTTAAAA
    CAACCAGCTC TTGGTCACCC AGGGCAGAGG CCAGATAAAG AGCCAAATCT AGCCTATGAA
    CCCCAACTTC CATATATAGA AAAACAAGCC AGCAGAGACC TTGAGCAGCC GTCATACAGG
    TATGAGGTCT CAAGCTACAC AGACCAGTTT TCTCGGAACT ATGACCATCG CCTACGGTTT
    GAAGATCGAA TCCCTACCTA TGAAGACCAG TGGTCATATT ATGATGACAA ACAGCCCTAC
    CAACCTCGGC CTTTTGAGAA TCAGCATCCC CGAGACCTGG ACTCCAGACA ACATCCCGAA
    GAGGCTTCAG AACGAGGTTA TTTCCAGCGT TTTGAAGAGC CAGCCCCTCT GTCGTACGAC
    AGTAGAACAC GCTATGAGCA GCTGCCTCGA ACCTCTACTC TACGACATGA AGAGCAGCCA
    GCCCCTGCAT ATGAGGTGCA CAACAGGTAC AGGCCAGAGG CACAGCCCTA TTCTTCAACA
    GGCCCTAAGT CATCTGAGCC CAAGCAGTAC TTTGACCAGT ACCCGCGAAG TTATGAGCAA
    GTTCCACCAC CAGGCTTTAC CTCCAAAACA GGCCATTACG AGCCTCTCCA TGGTGCTGCA
    GTTGTCCCTC CTCTGATACC TTCCTCTCAA CAAAAGCCAG AAGTCCTGCC CTCGGCTACC
    AAACCACAGC CTCCACCCCC AACCCTAACT GAGGAGGAGG AGGATCCAGC AATGAAACCA
    CAGTCTGTGC TCACCAGAGT CAAAATGTTT GAAAACAAAA GATCTGCGTC TTTGGAGAAC
    AAGAAAGATG TGAATGACAC TGCCAGCTTT AAGCCTCCGG AAGTAGCATC TAAACCTCCA
    GGTGCTTCTC TTGCTGGCCC TAAACCTGTC CCTCAGAGTC AGTTTAGTGA GCACGACAAA
    ACGCTCTACA GGCTCCCAGA GCCTCAGAAA CCTCAAGTGA AGCCACCCGA AGATATTGTT
    CGATCAAATC ATTACGACCC TGAAGAGGAT GAAGAATATT ACCGGAAACA GCTCTCCTAC
    TTTGACCGAA GAAGTTTTGA GAGCAAGCCT TCTGCACATC TTCCTGCTGG CCATCACTCA
    GAGCCTGCTA AGCCAGTCCA TTCTCAGAGC CAGCCCAACT TCTCTAGTTA CTCTTCAAAG
    GGAAAACCCG AAACTGATGC TGTGGATAGA TCATTCAGTG AGAAACGTTA TGATCCAGCC
    CAGGCCACGC CTCCTCCTCC TCCGTTGCCC TCACAGTACA GCCAGCCAGC TCCACCTCTG
    TCCAGCTCTT CTCTCCACAT ACATTCCAAG GGCGCCCAGG GTGAAGGCAA CTCAGTATCA
    TTGGATTTTC AGAACTCATA TATGTCCAAA CCAGACCCAC CCCCATCTCA GAGTAAACCA
    GCAACTTTCA GACCACCAAC TCGAGAAGAC CCCCCTCAGA CTTTCTATCC GCAGAAAAGT
    TTCCCAGACA AAGCTCCAGT TAACGGAGCT GAGCAGACTC AGAAAACCAT CACTCCGGTG
    TACAATCGAT TCACACCAAA GCCGTACACA AGTTCTGCCC GGCCATTTGA ACGCAAATTT
    GAAAGTCCGA AGTTCAACCA TAATCTTCTG CCAAGTGAAA CTGTACATAA ACCTGAATTG
    TCTTCAAAAA CTCCCACTTC CCCAAAAACT CTTATGAAAG CTCATAGTTC AACACAGCCT
    CCAGAGTTTG ACAGTGGAGT TGAAACTTTC TCTGTTCACA CAGATAAGCC TAAATATCAA
    ATGAATAATA TCAGCACCAT GCCTAAAGCT GTCCCTGTGA GTCCTTCAGC TGTGGAAGAA
    GATGAAGATG AGGATGGTCA TACTGTAGTG GCTACAGCTC GTGGCATTTT TAACAGCAAT
    GGTGGTGTGT TGAGTTCCAT AGAAACCGGT GTTAGTATAA TTATCCCACA AGGAGCCATT
    CCTGAAGGAA TTGAGCAAGA AATCTATTTC AAAGTCTGCA GAGACAATAG CATTCTCCCA
    CCTTTAGATA AAGAGAAAGG TGAAACTCTG CTGAGCCCCC TAGTGATGTG TGGGCCCCAT
    GGCCTCAAGT TCCTGAAGCC CGTGGAGCTA CGCTTGCCAC ACTGTGACCC TAAAACCTGG
    CAAAACAAGT GTCTTCCTGG AGATCCGAAT TACCTTGTTG GAGCCAACTG TGTTTCTGTC
    CTGATTGACC ACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu22535
    Clone ID Related Accession (Same CDS sequence) NM_001163574.1
    Accession Version NM_001163574.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5058bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-31
    Organism Mus musculus(house mouse)
    Product tight junction protein ZO-1 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ098724.1, BC138028.1 and BP760988.1. Transcript Variant: This variant (2) lacks an in-frame coding exon in the middle region but has an additional in-frame segment in the 3' coding region, as compared to variant 1. The resulting isoform (2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC138028.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCCGCCA GGGCCGCGGC CGCTAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
    CAGCACACAG TGACGCTTCA CAGGGCTCCT GGGTTTGGAT TTGGAATTGC AATCTCTGGT
    GGAAGAGATA ATCCTCATTT TCAGAGTGGG GAAACCTCCA TAGTGATTTC TGATGTGTTA
    AAAGGAGGGC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA
    GTTTCAATGG ATAACGTTGA ACATGCTTTT GCTGTTCAGC AGCTAAGGAA GAGTGGGAAA
    AACGCAAAAA TTACTATCCG AAGGAAGAAG AAAGTTCAGA TCCCTGTAAG TCACCCAGAT
    CCTGAGCCGG TGTCTGATAA TGAAGACGAT AGTTATGACG AAGAAGTGCA TGACCCAAGA
    GCTGGCCGCG GTGCTTTAGC GAACAGAAGG AGCGAGAAGA GCTGGGCAAG GGATAGGAGT
    GCAAGCAGGG AGAGGAGCCT GTCCCCTCGC TCGGACAGGC GGTCCGTGGC CTCCAGTCAG
    CCCGCAAAGC CCACCAAGGT CACACTGGTG AAGTCTCGGA AAAATGAAGA ATATGGTCTT
    CGATTGGCCA GCCATATATT TGTAAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT
    GGTAACATTC AAGAAGGGGA TGTTGTCTTG AAGATAAATG GTACTGTGAC AGAAAATATG
    TCATTGACAG ATGCAAAAAC ACTGATAGAA AGGTCTAAAG GCAAGTTAAA AATGGTAGTG
    CAAAGAGATG AGCGGGCTAC CTTACTGAAC GTCCCTGACC TTTCGGATAG TATCCATTCT
    GCTAATGCCT CGGAAAGAGA TGACATTTCA GAAATTCAGT CACTAGCATC AGACCATTCA
    GGTCGCTCGC ATGACAGGCC ACCCCGCCGC AGCCAGTCAC GATCTCCTGA CCAACGTTCA
    GAGCCCTCCG ATCATTCCAC GCAGTCTCCA CAGCAGCCCA GCAATGGCAG TCTCCGGAGC
    AGAGAGGAAG AGCGAATGTC TAAACCTGGG GCCATCTCAA CTCCTGTAAA ACATGTAGAC
    GATCATCCAC CCAAAGCAGT GGAAGAAGTT ACAGTTGAGA AAAATGAGAA GCAGACGCCC
    ACTCTTCCAG AACCGAAACC TGTGTATGCT CAAGTTGGAC AACCAGATGT GGATTTACCC
    GTCAGCCCTT CTGATGGTGC TCTGCCTAAT TCAGCTCATG AAGACGGGAT ACTTAGGCCC
    AGCATGAAAC TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGACT AGCTGGTGGA
    AATGATGTCG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAAGAAGGC
    TTAGAGGAAG GTGATCAAAT TCTCAGGGTG AACAATGTAG ATTTCACAAA TATCATAAGG
    GAAGAGGCCG TCCTTTTCCT CCTTGACCTC CCTAAAGGTG AAGAAGTGAC CATACTGGCT
    CAGAAGAAGA AGGACGTTTA TCGCCGCATT GTAGAATCAG ATGTAGGAGA TTCATTCTAT
    ATTAGAACGC ATTTTGAATA TGAAAAAGAA TCTCCTTACG GACTTAGTTT TAACAAAGGA
    GAGGTGTTCC GGGTCGTGGA TACTTTATAC AATGGAAAGC TGGGCTCTTG GCTTGCCATT
    CGAATTGGCA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT
    GAACAGTTAG CCAGTGTACA GTACACACTT CCAAAGACAG CGGGTGGTGA TCGGGCAGAC
    TTCTGGAGGT TTCGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAC
    TTGTCAGCTC AGCCAGTTCA AACTAAGTTC CCAGCTTATG AAAGGGTTGT TCTTCGAGAA
    GCTGGATTCC TAAGACCTGT AACCATCTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG
    TTGGCAAGAG AGGAGCCAGA TATCTATCAG ATTGCAAAAA GTGAACCACG AGATGCTGGG
    ACTGACCATC GTAGCTCTGG CATCATTCGC CTTCATACAA TAAAGCAAAT CATAGATCAG
    GATAAACATG CTTTATTAGA TGTAACGCCA AATGCGGTTG ATCGTCTTAA TTATGCGCAG
    TGGTATCCAA TTGTTGTGTT CCTTAACCCT GACTCTAAGC AAGGTGTAAA AACAATGAGG
    ATGAGGTTGT GTCCGGAGTC TCGGAAAAGC GCCAGGAAGC TATATGAACG CTCTCATAAG
    CTTCGTAAGA ACAATCACCA TCTCTTCACA ACTACAATTA ACTTAAACTC AATGAATGAT
    GGTTGGTACG GTGCCCTGAA AGAAGCGATT CAGCAGCAAC AGAACCAGCT GGTGTGGGTC
    TCTGAGGGGA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA CGATCGTCTG
    TCCTACCTGT CAGCCCCAGG TAGTGAGTAC TCAATGTATA GCACGGACAG TAGACACACT
    TCTGACTATG AAGACACAGA TACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA
    ACTCTTAATG ATGAGGTGGG GACTCCCCCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT
    GTAAGAGAGG ATTCCTCTGG AATGCATCAT GAAAACCAGA CATACCCTCC TTACTCACCA
    CAAGCGCAGC CACAAGCTAT TCATAGAATA GACTCCCCTG GACTTAAGCC AGCCTCTCAA
    CAGGTGTACA GGAAGGAGCC GTATTCTGAA GAAATGATGA GACAAAACCA TATTTTAAAA
    CAACCAGCTC TTGGTCACCC AGGGCAGAGG CCAGATAAAG AGCCAAATCT AGCCTATGAA
    CCCCAACTTC CATATATAGA AAAACAAGCC AGCAGAGACC TTGAGCAGCC GTCATACAGG
    TATGAGGTCT CAAGCTACAC AGACCAGTTT TCTCGGAACT ATGACCATCG CCTACGGTTT
    GAAGATCGAA TCCCTACCTA TGAAGACCAG TGGTCATATT ATGATGACAA ACAGCCCTAC
    CAACCTCGGC CTTTTGAGAA TCAGCATCCC CGAGACCTGG ACTCCAGACA ACATCCCGAA
    GAGGCTTCAG AACGAGGTTA TTTCCAGCGT TTTGAAGAGC CAGCCCCTCT GTCGTACGAC
    AGTAGAACAC GCTATGAGCA GCTGCCTCGA ACCTCTACTC TACGACATGA AGAGCAGCCA
    GCCCCTGCAT ATGAGGTGCA CAACAGGTAC AGGCCAGAGG CACAGCCCTA TTCTTCAACA
    GGCCCTAAGT CATCTGAGCC CAAGCAGTAC TTTGACCAGT ACCCGCGAAG TTATGAGCAA
    GTTCCACCAC CAGGCTTTAC CTCCAAAACA GGCCATTACG AGCCTCTCCA TGGTGCTGCA
    GTTGTCCCTC CTCTGATACC TTCCTCTCAA CAAAAGCCAG AAGTCCTGCC CTCGGCTACC
    AAACCACAGC CTCCACCCCC AACCCTAACT GAGGAGGAGG AGGATCCAGC AATGAAACCA
    CAGTCTGTGC TCACCAGAGT CAAAATGTTT GAAAACAAAA GATCTGCGTC TTTGGAGAAC
    AAGAAAGATG TGAATGACAC TGCCAGCTTT AAGCCTCCGG AAGTAGCATC TAAACCTCCA
    GGTGCTTCTC TTGCTGGCCC TAAACCTGTC CCTCAGAGTC AGTTTAGTGA GCACGACAAA
    ACGCTCTACA GGCTCCCAGA GCCTCAGAAA CCTCAAGTGA AGCCACCCGA AGATATTGTT
    CGATCAAATC ATTACGACCC TGAAGAGGAT GAAGAATATT ACCGGAAACA GCTCTCCTAC
    TTTGACCGAA GAAGTTTTGA GAGCAAGCCT TCTGCACATC TTCCTGCTGG CCATCACTCA
    GAGCCTGCTA AGCCAGTCCA TTCTCAGAGC CAGCCCAACT TCTCTAGTTA CTCTTCAAAG
    GGAAAACCCG AAACTGATGC TGTGGATAGA TCATTCAGTG AGAAACGTTA TGATCCAGCC
    CAGGCCACGC CTCCTCCTCC TCCGTTGCCC TCACAGTACA GCCAGCCAGC TCCACCTCTG
    TCCAGCTCTT CTCTCCACAT ACATTCCAAG GGCGCCCAGG GTGAAGGCAA CTCAGTATCA
    TTGGATTTTC AGAACTCATA TATGTCCAAA CCAGACCCAC CCCCATCTCA GAGTAAACCA
    GCAACTTTCA GACCACCAAC TCGAGAAGAC CCCCCTCAGA CTTTCTATCC GCAGAAAAGT
    TTCCCAGACA AAGCTCCAGT TAACGGAGCT GAGCAGACTC AGAAAACCAT CACTCCGGTG
    TACAATCGAT TCACACCAAA GCCGTACACA AGTTCTGCCC GGCCATTTGA ACGCAAATTT
    GAAAGTCCGA AGTTCAACCA TAATCTTCTG CCAAGTGAAA CTGTACATAA ACCTGAATTG
    TCTTCAAAAA CTCCCACTTC CCCAAAAACT CTTATGAAAG CTCATAGTTC AACACAGCCT
    CCAGAGTTTG ACAGTGGAGT TGAAACTTTC TCTGTTCACA CAGATAAGCC TAAATATCAA
    ATGAATAATA TCAGCACCAT GCCTAAAGCT GTCCCTGTGA GTCCTTCAGC TGTGGAAGAA
    GATGAAGATG AGGATGGTCA TACTGTAGTG GCTACAGCTC GTGGCATTTT TAACAGCAAT
    GGTGGTGTGT TGAGTTCCAT AGAAACCGGT GTTAGTATAA TTATCCCACA AGGAGCCATT
    CCTGAAGGAA TTGAGCAAGA AATCTATTTC AAAGTCTGCA GAGACAATAG CATTCTCCCA
    CCTTTAGATA AAGAGAAAGG TGAAACTCTG CTGAGCCCCC TAGTGATGTG TGGGCCCCAT
    GGCCTCAAGT TCCTGAAGCC CGTGGAGCTA CGCTTGCCAC ACTGTGCGTC CATGACTCCT
    GACGGTTGGT CTTTTGCTCT AAAATCATCC GACTCCTCGT CGGGTGACCC TAAAACCTGG
    CAAAACAAGT GTCTTCCTGG AGATCCGAAT TACCTTGTTG GAGCCAACTG TGTTTCTGTC
    CTGATTGACC ACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001157046.1
    CDS409..5466
    Translation

    Target ORF information:

    RefSeq Version NM_001163574.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus tight junction protein 1 (Tjp1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001163574.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCCGCCA GGGCCGCGGC CGCTAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
    CAGCACACAG TGACGCTTCA CAGGGCTCCT GGGTTTGGAT TTGGAATTGC AATCTCTGGT
    GGAAGAGATA ATCCTCATTT TCAGAGTGGG GAAACCTCCA TAGTGATTTC TGATGTGTTA
    AAAGGAGGGC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA
    GTTTCAATGG ATAACGTTGA ACATGCTTTT GCTGTTCAGC AGCTAAGGAA GAGTGGGAAA
    AACGCAAAAA TTACTATCCG AAGGAAGAAG AAAGTTCAGA TCCCTGTAAG TCACCCAGAT
    CCTGAGCCGG TGTCTGATAA TGAAGACGAT AGTTATGACG AAGAAGTGCA TGACCCAAGA
    GCTGGCCGCG GTGCTTTAGC GAACAGAAGG AGCGAGAAGA GCTGGGCAAG GGATAGGAGT
    GCAAGCAGGG AGAGGAGCCT GTCCCCTCGC TCGGACAGGC GGTCCGTGGC CTCCAGTCAG
    CCCGCAAAGC CCACCAAGGT CACACTGGTG AAGTCTCGGA AAAATGAAGA ATATGGTCTT
    CGATTGGCCA GCCATATATT TGTAAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT
    GGTAACATTC AAGAAGGGGA TGTTGTCTTG AAGATAAATG GTACTGTGAC AGAAAATATG
    TCATTGACAG ATGCAAAAAC ACTGATAGAA AGGTCTAAAG GCAAGTTAAA AATGGTAGTG
    CAAAGAGATG AGCGGGCTAC CTTACTGAAC GTCCCTGACC TTTCGGATAG TATCCATTCT
    GCTAATGCCT CGGAAAGAGA TGACATTTCA GAAATTCAGT CACTAGCATC AGACCATTCA
    GGTCGCTCGC ATGACAGGCC ACCCCGCCGC AGCCAGTCAC GATCTCCTGA CCAACGTTCA
    GAGCCCTCCG ATCATTCCAC GCAGTCTCCA CAGCAGCCCA GCAATGGCAG TCTCCGGAGC
    AGAGAGGAAG AGCGAATGTC TAAACCTGGG GCCATCTCAA CTCCTGTAAA ACATGTAGAC
    GATCATCCAC CCAAAGCAGT GGAAGAAGTT ACAGTTGAGA AAAATGAGAA GCAGACGCCC
    ACTCTTCCAG AACCGAAACC TGTGTATGCT CAAGTTGGAC AACCAGATGT GGATTTACCC
    GTCAGCCCTT CTGATGGTGC TCTGCCTAAT TCAGCTCATG AAGACGGGAT ACTTAGGCCC
    AGCATGAAAC TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGACT AGCTGGTGGA
    AATGATGTCG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAAGAAGGC
    TTAGAGGAAG GTGATCAAAT TCTCAGGGTG AACAATGTAG ATTTCACAAA TATCATAAGG
    GAAGAGGCCG TCCTTTTCCT CCTTGACCTC CCTAAAGGTG AAGAAGTGAC CATACTGGCT
    CAGAAGAAGA AGGACGTTTA TCGCCGCATT GTAGAATCAG ATGTAGGAGA TTCATTCTAT
    ATTAGAACGC ATTTTGAATA TGAAAAAGAA TCTCCTTACG GACTTAGTTT TAACAAAGGA
    GAGGTGTTCC GGGTCGTGGA TACTTTATAC AATGGAAAGC TGGGCTCTTG GCTTGCCATT
    CGAATTGGCA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT
    GAACAGTTAG CCAGTGTACA GTACACACTT CCAAAGACAG CGGGTGGTGA TCGGGCAGAC
    TTCTGGAGGT TTCGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAC
    TTGTCAGCTC AGCCAGTTCA AACTAAGTTC CCAGCTTATG AAAGGGTTGT TCTTCGAGAA
    GCTGGATTCC TAAGACCTGT AACCATCTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG
    TTGGCAAGAG AGGAGCCAGA TATCTATCAG ATTGCAAAAA GTGAACCACG AGATGCTGGG
    ACTGACCATC GTAGCTCTGG CATCATTCGC CTTCATACAA TAAAGCAAAT CATAGATCAG
    GATAAACATG CTTTATTAGA TGTAACGCCA AATGCGGTTG ATCGTCTTAA TTATGCGCAG
    TGGTATCCAA TTGTTGTGTT CCTTAACCCT GACTCTAAGC AAGGTGTAAA AACAATGAGG
    ATGAGGTTGT GTCCGGAGTC TCGGAAAAGC GCCAGGAAGC TATATGAACG CTCTCATAAG
    CTTCGTAAGA ACAATCACCA TCTCTTCACA ACTACAATTA ACTTAAACTC AATGAATGAT
    GGTTGGTACG GTGCCCTGAA AGAAGCGATT CAGCAGCAAC AGAACCAGCT GGTGTGGGTC
    TCTGAGGGGA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA CGATCGTCTG
    TCCTACCTGT CAGCCCCAGG TAGTGAGTAC TCAATGTATA GCACGGACAG TAGACACACT
    TCTGACTATG AAGACACAGA TACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA
    ACTCTTAATG ATGAGGTGGG GACTCCCCCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT
    GTAAGAGAGG ATTCCTCTGG AATGCATCAT GAAAACCAGA CATACCCTCC TTACTCACCA
    CAAGCGCAGC CACAAGCTAT TCATAGAATA GACTCCCCTG GACTTAAGCC AGCCTCTCAA
    CAGGTGTACA GGAAGGAGCC GTATTCTGAA GAAATGATGA GACAAAACCA TATTTTAAAA
    CAACCAGCTC TTGGTCACCC AGGGCAGAGG CCAGATAAAG AGCCAAATCT AGCCTATGAA
    CCCCAACTTC CATATATAGA AAAACAAGCC AGCAGAGACC TTGAGCAGCC GTCATACAGG
    TATGAGGTCT CAAGCTACAC AGACCAGTTT TCTCGGAACT ATGACCATCG CCTACGGTTT
    GAAGATCGAA TCCCTACCTA TGAAGACCAG TGGTCATATT ATGATGACAA ACAGCCCTAC
    CAACCTCGGC CTTTTGAGAA TCAGCATCCC CGAGACCTGG ACTCCAGACA ACATCCCGAA
    GAGGCTTCAG AACGAGGTTA TTTCCAGCGT TTTGAAGAGC CAGCCCCTCT GTCGTACGAC
    AGTAGAACAC GCTATGAGCA GCTGCCTCGA ACCTCTACTC TACGACATGA AGAGCAGCCA
    GCCCCTGCAT ATGAGGTGCA CAACAGGTAC AGGCCAGAGG CACAGCCCTA TTCTTCAACA
    GGCCCTAAGT CATCTGAGCC CAAGCAGTAC TTTGACCAGT ACCCGCGAAG TTATGAGCAA
    GTTCCACCAC CAGGCTTTAC CTCCAAAACA GGCCATTACG AGCCTCTCCA TGGTGCTGCA
    GTTGTCCCTC CTCTGATACC TTCCTCTCAA CAAAAGCCAG AAGTCCTGCC CTCGGCTACC
    AAACCACAGC CTCCACCCCC AACCCTAACT GAGGAGGAGG AGGATCCAGC AATGAAACCA
    CAGTCTGTGC TCACCAGAGT CAAAATGTTT GAAAACAAAA GATCTGCGTC TTTGGAGAAC
    AAGAAAGATG TGAATGACAC TGCCAGCTTT AAGCCTCCGG AAGTAGCATC TAAACCTCCA
    GGTGCTTCTC TTGCTGGCCC TAAACCTGTC CCTCAGAGTC AGTTTAGTGA GCACGACAAA
    ACGCTCTACA GGCTCCCAGA GCCTCAGAAA CCTCAAGTGA AGCCACCCGA AGATATTGTT
    CGATCAAATC ATTACGACCC TGAAGAGGAT GAAGAATATT ACCGGAAACA GCTCTCCTAC
    TTTGACCGAA GAAGTTTTGA GAGCAAGCCT TCTGCACATC TTCCTGCTGG CCATCACTCA
    GAGCCTGCTA AGCCAGTCCA TTCTCAGAGC CAGCCCAACT TCTCTAGTTA CTCTTCAAAG
    GGAAAACCCG AAACTGATGC TGTGGATAGA TCATTCAGTG AGAAACGTTA TGATCCAGCC
    CAGGCCACGC CTCCTCCTCC TCCGTTGCCC TCACAGTACA GCCAGCCAGC TCCACCTCTG
    TCCAGCTCTT CTCTCCACAT ACATTCCAAG GGCGCCCAGG GTGAAGGCAA CTCAGTATCA
    TTGGATTTTC AGAACTCATA TATGTCCAAA CCAGACCCAC CCCCATCTCA GAGTAAACCA
    GCAACTTTCA GACCACCAAC TCGAGAAGAC CCCCCTCAGA CTTTCTATCC GCAGAAAAGT
    TTCCCAGACA AAGCTCCAGT TAACGGAGCT GAGCAGACTC AGAAAACCAT CACTCCGGTG
    TACAATCGAT TCACACCAAA GCCGTACACA AGTTCTGCCC GGCCATTTGA ACGCAAATTT
    GAAAGTCCGA AGTTCAACCA TAATCTTCTG CCAAGTGAAA CTGTACATAA ACCTGAATTG
    TCTTCAAAAA CTCCCACTTC CCCAAAAACT CTTATGAAAG CTCATAGTTC AACACAGCCT
    CCAGAGTTTG ACAGTGGAGT TGAAACTTTC TCTGTTCACA CAGATAAGCC TAAATATCAA
    ATGAATAATA TCAGCACCAT GCCTAAAGCT GTCCCTGTGA GTCCTTCAGC TGTGGAAGAA
    GATGAAGATG AGGATGGTCA TACTGTAGTG GCTACAGCTC GTGGCATTTT TAACAGCAAT
    GGTGGTGTGT TGAGTTCCAT AGAAACCGGT GTTAGTATAA TTATCCCACA AGGAGCCATT
    CCTGAAGGAA TTGAGCAAGA AATCTATTTC AAAGTCTGCA GAGACAATAG CATTCTCCCA
    CCTTTAGATA AAGAGAAAGG TGAAACTCTG CTGAGCCCCC TAGTGATGTG TGGGCCCCAT
    GGCCTCAAGT TCCTGAAGCC CGTGGAGCTA CGCTTGCCAC ACTGTGCGTC CATGACTCCT
    GACGGTTGGT CTTTTGCTCT AAAATCATCC GACTCCTCGT CGGGTGACCC TAAAACCTGG
    CAAAACAAGT GTCTTCCTGG AGATCCGAAT TACCTTGTTG GAGCCAACTG TGTTTCTGTC
    CTGATTGACC ACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu38774
    Clone ID Related Accession (Same CDS sequence) XM_006540784.4 , XM_006540784.3
    Accession Version XM_006540784.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5349bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product tight junction protein ZO-1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006540784.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGTCCGCCA GGGCCGCGGC CGCTAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
    CAGCACACAG TGACGCTTCA CAGGGCTCCT GGGTTTGGAT TTGGAATTGC AATCTCTGGT
    GGAAGAGATA ATCCTCATTT TCAGAGTGGG GAAACCTCCA TAGTGATTTC TGATGTGTTA
    AAAGGAGGGC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA
    GTTTCAATGG ATAACGTTGA ACATGCTTTT GCTGTTCAGC AGCTAAGGAA GAGTGGGAAA
    AACGCAAAAA TTACTATCCG AAGGAAGAAG AAAGTTCAGA TCCCTGTAAG TCACCCAGAT
    CCTGAGCCGG TGTCTGATAA TGAAGACGAT AGTTATGACG AAGAAGTGCA TGACCCAAGA
    GCTGGCCGCG GTGCTTTAGC GAACAGAAGG AGCGAGAAGA GCTGGGCAAG GGATAGGAGT
    GCAAGCAGGG AGAGGAGCCT GTCCCCTCGC TCGGACAGGC GGTCCGTGGC CTCCAGTCAG
    CCCGCAAAGC CCACCAAGGT CACACTGGTG AAGTCTCGGA AAAATGAAGA ATATGGTCTT
    CGATTGGCCA GCCATATATT TGTAAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT
    GGTAACATTC AAGAAGGGGA TGTTGTCTTG AAGATAAATG GTACTGTGAC AGAAAATATG
    TCATTGACAG ATGCAAAAAC ACTGATAGAA AGGTCTAAAG GCAAGTTAAA AATGGTAGTG
    CAAAGAGATG AGCGGGCTAC CTTACTGAAC GTCCCTGACC TTTCGGATAG TATCCATTCT
    GCTAATGCCT CGGAAAGAGA TGACATTTCA GAAATTCAGT CACTAGCATC AGACCATTCA
    GGTCGCTCGC ATGACAGGCC ACCCCGCCGC AGCCAGTCAC GATCTCCTGA CCAACGTTCA
    GAGCCCTCCG ATCATTCCAC GCAGTCTCCA CAGCAGCCCA GCAATGGCAG TCTCCGGAGC
    AGAGAGGAAG AGCGAATGTC TAAACCTGGG GCCATCTCAA CTCCTGTAAA ACATGTAGAC
    GATCATCCAC CCAAAGCAGT GGAAGAAGTT ACAGTTGAGA AAAATGAGAA GCAGACGCCC
    ACTCTTCCAG AACCGAAACC TGTGTATGCT CAAGTTGGAC AACCAGATGT GGATTTACCC
    GTCAGCCCTT CTGATGGTGC TCTGCCTAAT TCAGCTCATG AAGACGGGAT ACTTAGGCCC
    AGCATGAAAC TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGACT AGCTGGTGGA
    AATGATGTCG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAAGAAGGC
    TTAGAGGAAG GTGATCAAAT TCTCAGGGTG AACAATGTAG ATTTCACAAA TATCATAAGG
    GAAGAGGCCG TCCTTTTCCT CCTTGACCTC CCTAAAGGTG AAGAAGTGAC CATACTGGCT
    CAGAAGAAGA AGGACGTTTA TCGCCGCATT GTAGAATCAG ATGTAGGAGA TTCATTCTAT
    ATTAGAACGC ATTTTGAATA TGAAAAAGAA TCTCCTTACG GACTTAGTTT TAACAAAGGA
    GAGGTGTTCC GGGTCGTGGA TACTTTATAC AATGGAAAGC TGGGCTCTTG GCTTGCCATT
    CGAATTGGCA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT
    GAACAGTTAG CCAGTGTACA GTACACACTT CCAAAGACAG CGGGTGGTGA TCGGGCAGAC
    TTCTGGAGGT TTCGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAC
    TTGTCAGCTC AGCCAGTTCA AACTAAGTTC CCAGCTTATG AAAGGGTTGT TCTTCGAGAA
    GCTGGATTCC TAAGACCTGT AACCATCTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG
    TTGGCAAGAG AGGAGCCAGA TATCTATCAG ATTGCAAAAA GTGAACCACG AGATGCTGGG
    ACTGACCATC GTAGCTCTGG CATCATTCGC CTTCATACAA TAAAGCAAAT CATAGATCAG
    GATAAACATG CTTTATTAGA TGTAACGCCA AATGCGGTTG ATCGTCTTAA TTATGCGCAG
    TGGTATCCAA TTGTTGTGTT CCTTAACCCT GACTCTAAGC AAGGTGTAAA AACAATGAGG
    ATGAGGTTGT GTCCGGAGTC TCGGAAAAGC GCCAGGAAGC TATATGAACG CTCTCATAAG
    CTTCGTAAGA ACAATCACCA TCTCTTCACA ACTACAATTA ACTTAAACTC AATGAATGAT
    GGTTGGTACG GTGCCCTGAA AGAAGCGATT CAGCAGCAAC AGAACCAGCT GGTGTGGGTC
    TCTGAGGGGA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA CGATCGTCTG
    TCCTACCTGT CAGCCCCAGG TAGTGAGTAC TCAATGTATA GCACGGACAG TAGACACACT
    TCTGACTATG AAGACACAGA TACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA
    ACTCTTAATG ATGAGGTGGG GACTCCCCCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT
    GTAAGAGAGG ATTCCTCTGG AATGCATCAT GAAAACCAGA CATACCCTCC TTACTCACCA
    CAAGCGCAGC CACAAGCTAT TCATAGAATA GACTCCCCTG GACTTAAGCC AGCCTCTCAA
    CAGAAAGCAG AAGCCTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAC CCCAGCATCA
    TCAGCCTCTG CAGTTAATCA TAATGTCAGT GTAACTAATG TCAGCCTGGA GGAGCCTGCC
    CCAGCCCCTC CCACCTCGCA CGCATCACAG CCTGGTTGTT TAGGAGCACC AAGTGCTGAG
    GCAGCTCACG TAGTGCTCAG AGGTGAAGGA CCACCATTGC CGCCGCATGC AGACCCAGCA
    AAGGTGTACA GGAAGGAGCC GTATTCTGAA GAAATGATGA GACAAAACCA TATTTTAAAA
    CAACCAGCTC TTGGTCACCC AGGGCAGAGG CCAGATAAAG AGCCAAATCT AGCCTATGAA
    CCCCAACTTC CATATATAGA AAAACAAGCC AGCAGAGACC TTGAGCAGCC GTCATACAGG
    TATGAGGTCT CAAGCTACAC AGACCAGTTT TCTCGGAACT ATGACCATCG CCTACGGTTT
    GAAGATCGAA TCCCTACCTA TGAAGACCAG TGGTCATATT ATGATGACAA ACAGCCCTAC
    CAACCTCGGC CTTTTGAGAA TCAGCATCCC CGAGACCTGG ACTCCAGACA ACATCCCGAA
    GAGGCTTCAG AACGAGGTTA TTTCCAGCGT TTTGAAGAGC CAGCCCCTCT GTCGTACGAC
    AGTAGAACAC GCTATGAGCA GCTGCCTCGA ACCTCTACTC TACGACATGA AGAGCAGCCA
    GCCCCTGCAT ATGAGGTGCA CAACAGGTAC AGGCCAGAGG CACAGCCCTA TTCTTCAACA
    GGCCCTAAGT CATCTGAGCC CAAGCAGTAC TTTGACCAGT ACCCGCGAAG TTATGAGCAA
    GTTCCACCAC CAGGCTTTAC CTCCAAAACA GGCCATTACG AGCCTCTCCA TGGTGCTGCA
    GTTGTCCCTC CTCTGATACC TTCCTCTCAA CAAAAGCCAG AAGTCCTGCC CTCGGCTACC
    AAACCACAGC CTCCACCCCC AACCCTAACT GAGGAGGAGG AGGATCCAGC AATGAAACCA
    CAGTCTGTGC TCACCAGAGT CAAAATGTTT GAAAACAAAA GATCTGCGTC TTTGGAGAAC
    AAGAAAGATG TGAATGACAC TGCCAGCTTT AAGCCTCCGG AAGTAGCATC TAAACCTCCA
    GGTGCTTCTC TTGCTGGCCC TAAACCTGTC CCTCAGAGTC AGTTTAGTGA GCACGACAAA
    ACGCTCTACA GGCTCCCAGA GCCTCAGAAA CCTCAAGTGA AGCCACCCGA AGATATTGTT
    CGATCAAATC ATTACGACCC TGAAGAGGAT GAAGAATATT ACCGGAAACA GCTCTCCTAC
    TTTGACCGAA GAAGTTTTGA GAGCAAGCCT TCTGCACATC TTCCTGCTGG CCATCACTCA
    GAGCCTGCTA AGCCAGTCCA TTCTCAGAGC CAGCCCAACT TCTCTAGTTA CTCTTCAAAA
    TTTCTTGGCT CTTACACTAG CTATGACTAC TTGAAAAGGA ACATCAAGTG GGGAAAACCC
    GAAACTGATG CTGTGGATAG ATCATTCAGT GAGAAACGTT ATGATCCAGC CCAGGCCACG
    CCTCCTCCTC CTCCGTTGCC CTCACAGTAC AGCCAGCCAG CTCCACCTCT GTCCAGCTCT
    TCTCTCCACA TACATTCCAA GGGCGCCCAG GGTGAAGGCA ACTCAGTATC ATTGGATTTT
    CAGAACTCAT ATATGTCCAA ACCAGACCCA CCCCCATCTC AGAGTAAACC AGCAACTTTC
    AGACCACCAA CTCGAGAAGA CCCCCCTCAG ACTTTCTATC CGCAGAAAAG TTTCCCAGAC
    AAAGCTCCAG TTAACGGAGC TGAGCAGACT CAGAAAACCA TCACTCCGGT GTACAATCGA
    TTCACACCAA AGCCGTACAC AAGTTCTGCC CGGCCATTTG AACGCAAATT TGAAAGTCCG
    AAGTTCAACC ATAATCTTCT GCCAAGTGAA ACTGTACATA AACCTGAATT GTCTTCAAAA
    ACTCCCACTT CCCCAAAAAC TCTTATGAAA GCTCATAGTT CAACACAGCC TCCAGAGTTT
    GACAGTGGAG TTGAAACTTT CTCTGTTCAC ACAGATAAGC CTAAATATCA AATGAATAAT
    ATCAGCACCA TGCCTAAAGC TGTCCCTGTG AGTCCTTCAG CTGTGGAAGA AGATGAAGAT
    GAGGATGGTC ATACTGTAGT GGCTACAGCT CGTGGCATTT TTAACAGCAA TGGTGGTGTG
    TTGAGTTCCA TAGAAACCGG TGTTAGTATA ATTATCCCAC AAGGAGCCAT TCCTGAAGGA
    ATTGAGCAAG AAATCTATTT CAAAGTCTGC AGAGACAATA GCATTCTCCC ACCTTTAGAT
    AAAGAGAAAG GTGAAACTCT GCTGAGCCCC CTAGTGATGT GTGGGCCCCA TGGCCTCAAG
    TTCCTGAAGC CCGTGGAGCT ACGCTTGCCA CACTGTGCGT CCATGACTCC TGACGGTTGG
    TCTTTTGCTC TAAAATCATC CGACTCCTCG TCGGGTGACC CTAAAACCTG GCAAAACAAG
    TGTCTTCCTG GAGATCCGAA TTACCTTGTT GGAGCCAACT GTGTTTCTGT CCTGATTGAC
    CACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006540847.1
    CDS846..6194
    Translation

    Target ORF information:

    RefSeq Version XM_006540784.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus tight junction protein 1 (Tjp1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006540784.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGTCCGCCA GGGCCGCGGC CGCTAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
    CAGCACACAG TGACGCTTCA CAGGGCTCCT GGGTTTGGAT TTGGAATTGC AATCTCTGGT
    GGAAGAGATA ATCCTCATTT TCAGAGTGGG GAAACCTCCA TAGTGATTTC TGATGTGTTA
    AAAGGAGGGC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA
    GTTTCAATGG ATAACGTTGA ACATGCTTTT GCTGTTCAGC AGCTAAGGAA GAGTGGGAAA
    AACGCAAAAA TTACTATCCG AAGGAAGAAG AAAGTTCAGA TCCCTGTAAG TCACCCAGAT
    CCTGAGCCGG TGTCTGATAA TGAAGACGAT AGTTATGACG AAGAAGTGCA TGACCCAAGA
    GCTGGCCGCG GTGCTTTAGC GAACAGAAGG AGCGAGAAGA GCTGGGCAAG GGATAGGAGT
    GCAAGCAGGG AGAGGAGCCT GTCCCCTCGC TCGGACAGGC GGTCCGTGGC CTCCAGTCAG
    CCCGCAAAGC CCACCAAGGT CACACTGGTG AAGTCTCGGA AAAATGAAGA ATATGGTCTT
    CGATTGGCCA GCCATATATT TGTAAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT
    GGTAACATTC AAGAAGGGGA TGTTGTCTTG AAGATAAATG GTACTGTGAC AGAAAATATG
    TCATTGACAG ATGCAAAAAC ACTGATAGAA AGGTCTAAAG GCAAGTTAAA AATGGTAGTG
    CAAAGAGATG AGCGGGCTAC CTTACTGAAC GTCCCTGACC TTTCGGATAG TATCCATTCT
    GCTAATGCCT CGGAAAGAGA TGACATTTCA GAAATTCAGT CACTAGCATC AGACCATTCA
    GGTCGCTCGC ATGACAGGCC ACCCCGCCGC AGCCAGTCAC GATCTCCTGA CCAACGTTCA
    GAGCCCTCCG ATCATTCCAC GCAGTCTCCA CAGCAGCCCA GCAATGGCAG TCTCCGGAGC
    AGAGAGGAAG AGCGAATGTC TAAACCTGGG GCCATCTCAA CTCCTGTAAA ACATGTAGAC
    GATCATCCAC CCAAAGCAGT GGAAGAAGTT ACAGTTGAGA AAAATGAGAA GCAGACGCCC
    ACTCTTCCAG AACCGAAACC TGTGTATGCT CAAGTTGGAC AACCAGATGT GGATTTACCC
    GTCAGCCCTT CTGATGGTGC TCTGCCTAAT TCAGCTCATG AAGACGGGAT ACTTAGGCCC
    AGCATGAAAC TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGACT AGCTGGTGGA
    AATGATGTCG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAAGAAGGC
    TTAGAGGAAG GTGATCAAAT TCTCAGGGTG AACAATGTAG ATTTCACAAA TATCATAAGG
    GAAGAGGCCG TCCTTTTCCT CCTTGACCTC CCTAAAGGTG AAGAAGTGAC CATACTGGCT
    CAGAAGAAGA AGGACGTTTA TCGCCGCATT GTAGAATCAG ATGTAGGAGA TTCATTCTAT
    ATTAGAACGC ATTTTGAATA TGAAAAAGAA TCTCCTTACG GACTTAGTTT TAACAAAGGA
    GAGGTGTTCC GGGTCGTGGA TACTTTATAC AATGGAAAGC TGGGCTCTTG GCTTGCCATT
    CGAATTGGCA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT
    GAACAGTTAG CCAGTGTACA GTACACACTT CCAAAGACAG CGGGTGGTGA TCGGGCAGAC
    TTCTGGAGGT TTCGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAC
    TTGTCAGCTC AGCCAGTTCA AACTAAGTTC CCAGCTTATG AAAGGGTTGT TCTTCGAGAA
    GCTGGATTCC TAAGACCTGT AACCATCTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG
    TTGGCAAGAG AGGAGCCAGA TATCTATCAG ATTGCAAAAA GTGAACCACG AGATGCTGGG
    ACTGACCATC GTAGCTCTGG CATCATTCGC CTTCATACAA TAAAGCAAAT CATAGATCAG
    GATAAACATG CTTTATTAGA TGTAACGCCA AATGCGGTTG ATCGTCTTAA TTATGCGCAG
    TGGTATCCAA TTGTTGTGTT CCTTAACCCT GACTCTAAGC AAGGTGTAAA AACAATGAGG
    ATGAGGTTGT GTCCGGAGTC TCGGAAAAGC GCCAGGAAGC TATATGAACG CTCTCATAAG
    CTTCGTAAGA ACAATCACCA TCTCTTCACA ACTACAATTA ACTTAAACTC AATGAATGAT
    GGTTGGTACG GTGCCCTGAA AGAAGCGATT CAGCAGCAAC AGAACCAGCT GGTGTGGGTC
    TCTGAGGGGA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA CGATCGTCTG
    TCCTACCTGT CAGCCCCAGG TAGTGAGTAC TCAATGTATA GCACGGACAG TAGACACACT
    TCTGACTATG AAGACACAGA TACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA
    ACTCTTAATG ATGAGGTGGG GACTCCCCCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT
    GTAAGAGAGG ATTCCTCTGG AATGCATCAT GAAAACCAGA CATACCCTCC TTACTCACCA
    CAAGCGCAGC CACAAGCTAT TCATAGAATA GACTCCCCTG GACTTAAGCC AGCCTCTCAA
    CAGAAAGCAG AAGCCTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAC CCCAGCATCA
    TCAGCCTCTG CAGTTAATCA TAATGTCAGT GTAACTAATG TCAGCCTGGA GGAGCCTGCC
    CCAGCCCCTC CCACCTCGCA CGCATCACAG CCTGGTTGTT TAGGAGCACC AAGTGCTGAG
    GCAGCTCACG TAGTGCTCAG AGGTGAAGGA CCACCATTGC CGCCGCATGC AGACCCAGCA
    AAGGTGTACA GGAAGGAGCC GTATTCTGAA GAAATGATGA GACAAAACCA TATTTTAAAA
    CAACCAGCTC TTGGTCACCC AGGGCAGAGG CCAGATAAAG AGCCAAATCT AGCCTATGAA
    CCCCAACTTC CATATATAGA AAAACAAGCC AGCAGAGACC TTGAGCAGCC GTCATACAGG
    TATGAGGTCT CAAGCTACAC AGACCAGTTT TCTCGGAACT ATGACCATCG CCTACGGTTT
    GAAGATCGAA TCCCTACCTA TGAAGACCAG TGGTCATATT ATGATGACAA ACAGCCCTAC
    CAACCTCGGC CTTTTGAGAA TCAGCATCCC CGAGACCTGG ACTCCAGACA ACATCCCGAA
    GAGGCTTCAG AACGAGGTTA TTTCCAGCGT TTTGAAGAGC CAGCCCCTCT GTCGTACGAC
    AGTAGAACAC GCTATGAGCA GCTGCCTCGA ACCTCTACTC TACGACATGA AGAGCAGCCA
    GCCCCTGCAT ATGAGGTGCA CAACAGGTAC AGGCCAGAGG CACAGCCCTA TTCTTCAACA
    GGCCCTAAGT CATCTGAGCC CAAGCAGTAC TTTGACCAGT ACCCGCGAAG TTATGAGCAA
    GTTCCACCAC CAGGCTTTAC CTCCAAAACA GGCCATTACG AGCCTCTCCA TGGTGCTGCA
    GTTGTCCCTC CTCTGATACC TTCCTCTCAA CAAAAGCCAG AAGTCCTGCC CTCGGCTACC
    AAACCACAGC CTCCACCCCC AACCCTAACT GAGGAGGAGG AGGATCCAGC AATGAAACCA
    CAGTCTGTGC TCACCAGAGT CAAAATGTTT GAAAACAAAA GATCTGCGTC TTTGGAGAAC
    AAGAAAGATG TGAATGACAC TGCCAGCTTT AAGCCTCCGG AAGTAGCATC TAAACCTCCA
    GGTGCTTCTC TTGCTGGCCC TAAACCTGTC CCTCAGAGTC AGTTTAGTGA GCACGACAAA
    ACGCTCTACA GGCTCCCAGA GCCTCAGAAA CCTCAAGTGA AGCCACCCGA AGATATTGTT
    CGATCAAATC ATTACGACCC TGAAGAGGAT GAAGAATATT ACCGGAAACA GCTCTCCTAC
    TTTGACCGAA GAAGTTTTGA GAGCAAGCCT TCTGCACATC TTCCTGCTGG CCATCACTCA
    GAGCCTGCTA AGCCAGTCCA TTCTCAGAGC CAGCCCAACT TCTCTAGTTA CTCTTCAAAA
    TTTCTTGGCT CTTACACTAG CTATGACTAC TTGAAAAGGA ACATCAAGTG GGGAAAACCC
    GAAACTGATG CTGTGGATAG ATCATTCAGT GAGAAACGTT ATGATCCAGC CCAGGCCACG
    CCTCCTCCTC CTCCGTTGCC CTCACAGTAC AGCCAGCCAG CTCCACCTCT GTCCAGCTCT
    TCTCTCCACA TACATTCCAA GGGCGCCCAG GGTGAAGGCA ACTCAGTATC ATTGGATTTT
    CAGAACTCAT ATATGTCCAA ACCAGACCCA CCCCCATCTC AGAGTAAACC AGCAACTTTC
    AGACCACCAA CTCGAGAAGA CCCCCCTCAG ACTTTCTATC CGCAGAAAAG TTTCCCAGAC
    AAAGCTCCAG TTAACGGAGC TGAGCAGACT CAGAAAACCA TCACTCCGGT GTACAATCGA
    TTCACACCAA AGCCGTACAC AAGTTCTGCC CGGCCATTTG AACGCAAATT TGAAAGTCCG
    AAGTTCAACC ATAATCTTCT GCCAAGTGAA ACTGTACATA AACCTGAATT GTCTTCAAAA
    ACTCCCACTT CCCCAAAAAC TCTTATGAAA GCTCATAGTT CAACACAGCC TCCAGAGTTT
    GACAGTGGAG TTGAAACTTT CTCTGTTCAC ACAGATAAGC CTAAATATCA AATGAATAAT
    ATCAGCACCA TGCCTAAAGC TGTCCCTGTG AGTCCTTCAG CTGTGGAAGA AGATGAAGAT
    GAGGATGGTC ATACTGTAGT GGCTACAGCT CGTGGCATTT TTAACAGCAA TGGTGGTGTG
    TTGAGTTCCA TAGAAACCGG TGTTAGTATA ATTATCCCAC AAGGAGCCAT TCCTGAAGGA
    ATTGAGCAAG AAATCTATTT CAAAGTCTGC AGAGACAATA GCATTCTCCC ACCTTTAGAT
    AAAGAGAAAG GTGAAACTCT GCTGAGCCCC CTAGTGATGT GTGGGCCCCA TGGCCTCAAG
    TTCCTGAAGC CCGTGGAGCT ACGCTTGCCA CACTGTGCGT CCATGACTCC TGACGGTTGG
    TCTTTTGCTC TAAAATCATC CGACTCCTCG TCGGGTGACC CTAAAACCTG GCAAAACAAG
    TGTCTTCCTG GAGATCCGAA TTACCTTGTT GGAGCCAACT GTGTTTCTGT CCTGATTGAC
    CACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu38768
    Clone ID Related Accession (Same CDS sequence) XM_006540778.3 , XM_006540778.4
    Accession Version XM_006540778.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5628bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product tight junction protein ZO-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006540778.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT
    CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA
    CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC
    AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA
    CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA
    AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT
    GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG
    AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG
    TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC
    ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT
    GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT
    GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA
    CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC
    TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT
    GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA
    CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG
    CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT
    AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG
    GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT
    GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT
    CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC
    CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT
    CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT
    GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT
    ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG
    TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA
    ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA
    ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT
    ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC
    ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT
    GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC
    CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT
    CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT
    CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA
    GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT
    AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG
    ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA
    ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT
    CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCCTCATCT
    CCAGTCCCTT ACCTTTCGCC TGAAACAACC CCAGCATCAT CAGCCTCTGC AGTTAATCAT
    AATGTCAGTG TAACTAATGT CAGCCTGGAG GAGCCTGCCC CAGCCCCTCC CACCTCGCAC
    GCATCACAGC CTGGTTGTTT AGGAGCACCA AGTGCTGAGG CAGCTCACGT AGTGCTCAGA
    GGTGAAGGAC CACCATTGCC GCCGCATGCA GACCCAGCAA AGGTGTACAG GAAGGAGCCG
    TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA
    GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA
    AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA
    GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT
    GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT
    CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT
    TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG
    CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC
    AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC
    AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC
    TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT
    TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA
    ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC
    AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT
    GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT
    AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG
    CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT
    GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG
    AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT
    TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAAT TTCTTGGCTC TTACACTAGC
    TATGACTACT TGAAAAGGAA CATCAAGTGG GGAAAACCCG AAACTGATGC TGTGGATAGA
    TCATTCAGTG AGAAACGTTA TGATCCAGCC CAGGCCACGC CTCCTCCTCC TCCGTTGCCC
    TCACAGTACA GCCAGCCAGC TCCACCTCTG TCCAGCTCTT CTCTCCACAT ACATTCCAAG
    GGCGCCCAGG GTGAAGGCAA CTCAGTATCA TTGGATTTTC AGAACTCATA TATGTCCAAA
    CCAGACCCAC CCCCATCTCA GAGTAAACCA GCAACTTTCA GACCACCAAC TCGAGAAGAC
    CCCCCTCAGA CTTTCTATCC GCAGAAAAGT TTCCCAGACA AAGCTCCAGT TAACGGAGCT
    GAGCAGACTC AGAAAACCAT CACTCCGGTG TACAATCGAT TCACACCAAA GCCGTACACA
    AGTTCTGCCC GGCCATTTGA ACGCAAATTT GAAAGTCCGA AGTTCAACCA TAATCTTCTG
    CCAAGTGAAA CTGTACATAA ACCTGAATTG TCTTCAAAAA CTCCCACTTC CCCAAAAACT
    CTTATGAAAG CTCATAGTTC AACACAGCCT CCAGAGTTTG ACAGTGGAGT TGAAACTTTC
    TCTGTTCACA CAGATAAGCC TAAATATCAA ATGAATAATA TCAGCACCAT GCCTAAAGCT
    GTCCCTGTGA GTCCTTCAGC TGTGGAAGAA GATGAAGATG AGGATGGTCA TACTGTAGTG
    GCTACAGCTC GTGGCATTTT TAACAGCAAT GGTGGTGTGT TGAGTTCCAT AGAAACCGGT
    GTTAGTATAA TTATCCCACA AGGAGCCATT CCTGAAGGAA TTGAGCAAGA AATCTATTTC
    AAAGTCTGCA GAGACAATAG CATTCTCCCA CCTTTAGATA AAGAGAAAGG TGAAACTCTG
    CTGAGCCCCC TAGTGATGTG TGGGCCCCAT GGCCTCAAGT TCCTGAAGCC CGTGGAGCTA
    CGCTTGCCAC ACTGTGCGTC CATGACTCCT GACGGTTGGT CTTTTGCTCT AAAATCATCC
    GACTCCTCGT CGGGTGACCC TAAAACCTGG CAAAACAAGT GTCTTCCTGG AGATCCGAAT
    TACCTTGTTG GAGCCAACTG TGTTTCTGTC CTGATTGACC ACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006540841.1
    CDS73..5700
    Translation

    Target ORF information:

    RefSeq Version XM_006540778.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus tight junction protein 1 (Tjp1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006540778.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT
    CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA
    CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC
    AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA
    CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA
    AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT
    GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG
    AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG
    TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC
    ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT
    GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT
    GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA
    CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC
    TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT
    GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA
    CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG
    CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT
    AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG
    GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT
    GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT
    CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC
    CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT
    CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT
    GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT
    ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG
    TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA
    ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA
    ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT
    ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC
    ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT
    GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC
    CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT
    CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT
    CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA
    GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT
    AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG
    ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA
    ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT
    CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCCTCATCT
    CCAGTCCCTT ACCTTTCGCC TGAAACAACC CCAGCATCAT CAGCCTCTGC AGTTAATCAT
    AATGTCAGTG TAACTAATGT CAGCCTGGAG GAGCCTGCCC CAGCCCCTCC CACCTCGCAC
    GCATCACAGC CTGGTTGTTT AGGAGCACCA AGTGCTGAGG CAGCTCACGT AGTGCTCAGA
    GGTGAAGGAC CACCATTGCC GCCGCATGCA GACCCAGCAA AGGTGTACAG GAAGGAGCCG
    TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA
    GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA
    AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA
    GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT
    GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT
    CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT
    TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG
    CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC
    AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC
    AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC
    TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT
    TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA
    ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC
    AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT
    GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT
    AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG
    CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT
    GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG
    AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT
    TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAAT TTCTTGGCTC TTACACTAGC
    TATGACTACT TGAAAAGGAA CATCAAGTGG GGAAAACCCG AAACTGATGC TGTGGATAGA
    TCATTCAGTG AGAAACGTTA TGATCCAGCC CAGGCCACGC CTCCTCCTCC TCCGTTGCCC
    TCACAGTACA GCCAGCCAGC TCCACCTCTG TCCAGCTCTT CTCTCCACAT ACATTCCAAG
    GGCGCCCAGG GTGAAGGCAA CTCAGTATCA TTGGATTTTC AGAACTCATA TATGTCCAAA
    CCAGACCCAC CCCCATCTCA GAGTAAACCA GCAACTTTCA GACCACCAAC TCGAGAAGAC
    CCCCCTCAGA CTTTCTATCC GCAGAAAAGT TTCCCAGACA AAGCTCCAGT TAACGGAGCT
    GAGCAGACTC AGAAAACCAT CACTCCGGTG TACAATCGAT TCACACCAAA GCCGTACACA
    AGTTCTGCCC GGCCATTTGA ACGCAAATTT GAAAGTCCGA AGTTCAACCA TAATCTTCTG
    CCAAGTGAAA CTGTACATAA ACCTGAATTG TCTTCAAAAA CTCCCACTTC CCCAAAAACT
    CTTATGAAAG CTCATAGTTC AACACAGCCT CCAGAGTTTG ACAGTGGAGT TGAAACTTTC
    TCTGTTCACA CAGATAAGCC TAAATATCAA ATGAATAATA TCAGCACCAT GCCTAAAGCT
    GTCCCTGTGA GTCCTTCAGC TGTGGAAGAA GATGAAGATG AGGATGGTCA TACTGTAGTG
    GCTACAGCTC GTGGCATTTT TAACAGCAAT GGTGGTGTGT TGAGTTCCAT AGAAACCGGT
    GTTAGTATAA TTATCCCACA AGGAGCCATT CCTGAAGGAA TTGAGCAAGA AATCTATTTC
    AAAGTCTGCA GAGACAATAG CATTCTCCCA CCTTTAGATA AAGAGAAAGG TGAAACTCTG
    CTGAGCCCCC TAGTGATGTG TGGGCCCCAT GGCCTCAAGT TCCTGAAGCC CGTGGAGCTA
    CGCTTGCCAC ACTGTGCGTC CATGACTCCT GACGGTTGGT CTTTTGCTCT AAAATCATCC
    GACTCCTCGT CGGGTGACCC TAAAACCTGG CAAAACAAGT GTCTTCCTGG AGATCCGAAT
    TACCTTGTTG GAGCCAACTG TGTTTCTGTC CTGATTGACC ACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu38769
    Clone ID Related Accession (Same CDS sequence) XM_006540779.3 , XM_006540779.4
    Accession Version XM_006540779.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5589bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product tight junction protein ZO-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006540779.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT
    CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA
    CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC
    AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA
    CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA
    AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT
    GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG
    AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG
    TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC
    ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT
    GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT
    GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA
    CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC
    TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT
    GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA
    CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG
    CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT
    AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG
    GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT
    GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT
    CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC
    CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT
    CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT
    GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT
    ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG
    TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA
    ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA
    ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT
    ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC
    ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT
    GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC
    CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT
    CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT
    CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA
    GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT
    AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG
    ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA
    ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT
    CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCCTCATCT
    CCAGTCCCTT ACCTTTCGCC TGAAACAACC CCAGCATCAT CAGCCTCTGC AGTTAATCAT
    AATGTCAGTG TAACTAATGT CAGCCTGGAG GAGCCTGCCC CAGCCCCTCC CACCTCGCAC
    GCATCACAGC CTGGTTGTTT AGGAGCACCA AGTGCTGAGG CAGCTCACGT AGTGCTCAGA
    GGTGAAGGAC CACCATTGCC GCCGCATGCA GACCCAGCAA AGGTGTACAG GAAGGAGCCG
    TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA
    GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA
    AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA
    GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT
    GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT
    CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT
    TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG
    CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC
    AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC
    AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC
    TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT
    TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA
    ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC
    AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT
    GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT
    AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG
    CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT
    GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG
    AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT
    TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAAT TTCTTGGCTC TTACACTAGC
    TATGACTACT TGAAAAGGAA CATCAAGTGG GGAAAACCCG AAACTGATGC TGTGGATAGA
    TCATTCAGTG AGAAACGTTA TGATCCAGCC CAGGCCACGC CTCCTCCTCC TCCGTTGCCC
    TCACAGTACA GCCAGCCAGC TCCACCTCTG TCCAGCTCTT CTCTCCACAT ACATTCCAAG
    GGCGCCCAGG GTGAAGGCAA CTCAGTATCA TTGGATTTTC AGAACTCATA TATGTCCAAA
    CCAGACCCAC CCCCATCTCA GAGTAAACCA GCAACTTTCA GACCACCAAC TCGAGAAGAC
    CCCCCTCAGA CTTTCTATCC GCAGAAAAGT TTCCCAGACA AAGCTCCAGT TAACGGAGCT
    GAGCAGACTC AGAAAACCAT CACTCCGGTG TACAATCGAT TCACACCAAA GCCGTACACA
    AGTTCTGCCC GGCCATTTGA ACGCAAATTT GAAAGTCCGA AGTTCAACCA TAATCTTCTG
    CCAAGTGAAA CTGTACATAA ACCTGAATTG TCTTCAAAAA CTCCCACTTC CCCAAAAACT
    CTTATGAAAG CTCATAGTTC AACACAGCCT CCAGAGTTTG ACAGTGGAGT TGAAACTTTC
    TCTGTTCACA CAGATAAGCC TAAATATCAA ATGAATAATA TCAGCACCAT GCCTAAAGCT
    GTCCCTGTGA GTCCTTCAGC TGTGGAAGAA GATGAAGATG AGGATGGTCA TACTGTAGTG
    GCTACAGCTC GTGGCATTTT TAACAGCAAT GGTGGTGTGT TGAGTTCCAT AGAAACCGGT
    GTTAGTATAA TTATCCCACA AGGAGCCATT CCTGAAGGAA TTGAGCAAGA AATCTATTTC
    AAAGTCTGCA GAGACAATAG CATTCTCCCA CCTTTAGATA AAGAGAAAGG TGAAACTCTG
    CTGAGCCCCC TAGTGATGTG TGGGCCCCAT GGCCTCAAGT TCCTGAAGCC CGTGGAGCTA
    CGCTTGCCAC ACTGTGCGTC CATGACTCCT GACGGTGACC CTAAAACCTG GCAAAACAAG
    TGTCTTCCTG GAGATCCGAA TTACCTTGTT GGAGCCAACT GTGTTTCTGT CCTGATTGAC
    CACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006540842.1
    CDS73..5661
    Translation

    Target ORF information:

    RefSeq Version XM_006540779.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus tight junction protein 1 (Tjp1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006540779.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT
    CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA
    CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC
    AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA
    CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA
    AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT
    GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG
    AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG
    TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC
    ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT
    GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT
    GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA
    CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC
    TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT
    GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA
    CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG
    CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT
    AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG
    GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT
    GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT
    CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC
    CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT
    CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT
    GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT
    ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG
    TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA
    ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA
    ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT
    ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC
    ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT
    GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC
    CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT
    CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT
    CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA
    GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT
    AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG
    ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA
    ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT
    CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCCTCATCT
    CCAGTCCCTT ACCTTTCGCC TGAAACAACC CCAGCATCAT CAGCCTCTGC AGTTAATCAT
    AATGTCAGTG TAACTAATGT CAGCCTGGAG GAGCCTGCCC CAGCCCCTCC CACCTCGCAC
    GCATCACAGC CTGGTTGTTT AGGAGCACCA AGTGCTGAGG CAGCTCACGT AGTGCTCAGA
    GGTGAAGGAC CACCATTGCC GCCGCATGCA GACCCAGCAA AGGTGTACAG GAAGGAGCCG
    TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA
    GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA
    AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA
    GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT
    GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT
    CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT
    TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG
    CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC
    AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC
    AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC
    TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT
    TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA
    ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC
    AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT
    GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT
    AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG
    CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT
    GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG
    AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT
    TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAAT TTCTTGGCTC TTACACTAGC
    TATGACTACT TGAAAAGGAA CATCAAGTGG GGAAAACCCG AAACTGATGC TGTGGATAGA
    TCATTCAGTG AGAAACGTTA TGATCCAGCC CAGGCCACGC CTCCTCCTCC TCCGTTGCCC
    TCACAGTACA GCCAGCCAGC TCCACCTCTG TCCAGCTCTT CTCTCCACAT ACATTCCAAG
    GGCGCCCAGG GTGAAGGCAA CTCAGTATCA TTGGATTTTC AGAACTCATA TATGTCCAAA
    CCAGACCCAC CCCCATCTCA GAGTAAACCA GCAACTTTCA GACCACCAAC TCGAGAAGAC
    CCCCCTCAGA CTTTCTATCC GCAGAAAAGT TTCCCAGACA AAGCTCCAGT TAACGGAGCT
    GAGCAGACTC AGAAAACCAT CACTCCGGTG TACAATCGAT TCACACCAAA GCCGTACACA
    AGTTCTGCCC GGCCATTTGA ACGCAAATTT GAAAGTCCGA AGTTCAACCA TAATCTTCTG
    CCAAGTGAAA CTGTACATAA ACCTGAATTG TCTTCAAAAA CTCCCACTTC CCCAAAAACT
    CTTATGAAAG CTCATAGTTC AACACAGCCT CCAGAGTTTG ACAGTGGAGT TGAAACTTTC
    TCTGTTCACA CAGATAAGCC TAAATATCAA ATGAATAATA TCAGCACCAT GCCTAAAGCT
    GTCCCTGTGA GTCCTTCAGC TGTGGAAGAA GATGAAGATG AGGATGGTCA TACTGTAGTG
    GCTACAGCTC GTGGCATTTT TAACAGCAAT GGTGGTGTGT TGAGTTCCAT AGAAACCGGT
    GTTAGTATAA TTATCCCACA AGGAGCCATT CCTGAAGGAA TTGAGCAAGA AATCTATTTC
    AAAGTCTGCA GAGACAATAG CATTCTCCCA CCTTTAGATA AAGAGAAAGG TGAAACTCTG
    CTGAGCCCCC TAGTGATGTG TGGGCCCCAT GGCCTCAAGT TCCTGAAGCC CGTGGAGCTA
    CGCTTGCCAC ACTGTGCGTC CATGACTCCT GACGGTGACC CTAAAACCTG GCAAAACAAG
    TGTCTTCCTG GAGATCCGAA TTACCTTGTT GGAGCCAACT GTGTTTCTGT CCTGATTGAC
    CACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu38770
    Clone ID Related Accession (Same CDS sequence) XM_006540780.4 , XM_006540780.3
    Accession Version XM_006540780.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5577bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product tight junction protein ZO-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006540780.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT
    CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA
    CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC
    AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA
    CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA
    AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT
    GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG
    AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG
    TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC
    ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT
    GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT
    GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA
    CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC
    TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT
    GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA
    CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG
    CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT
    AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG
    GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT
    GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT
    CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC
    CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT
    CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT
    GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT
    ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG
    TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA
    ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA
    ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT
    ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC
    ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT
    GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC
    CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT
    CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT
    CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA
    GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT
    AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG
    ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA
    ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT
    CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCCTCATCT
    CCAGTCCCTT ACCTTTCGCC TGAAACAACC CCAGCATCAT CAGCCTCTGC AGTTAATCAT
    AATGTCAGTG TAACTAATGT CAGCCTGGAG GAGCCTGCCC CAGCCCCTCC CACCTCGCAC
    GCATCACAGC CTGGTTGTTT AGGAGCACCA AGTGCTGAGG CAGCTCACGT AGTGCTCAGA
    GGTGAAGGAC CACCATTGCC GCCGCATGCA GACCCAGCAA AGGTGTACAG GAAGGAGCCG
    TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA
    GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA
    AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA
    GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT
    GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT
    CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT
    TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG
    CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC
    AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC
    AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC
    TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT
    TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA
    ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC
    AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT
    GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT
    AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG
    CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT
    GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG
    AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT
    TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAGG GAAAACCCGA AACTGATGCT
    GTGGATAGAT CATTCAGTGA GAAACGTTAT GATCCAGCCC AGGCCACGCC TCCTCCTCCT
    CCGTTGCCCT CACAGTACAG CCAGCCAGCT CCACCTCTGT CCAGCTCTTC TCTCCACATA
    CATTCCAAGG GCGCCCAGGG TGAAGGCAAC TCAGTATCAT TGGATTTTCA GAACTCATAT
    ATGTCCAAAC CAGACCCACC CCCATCTCAG AGTAAACCAG CAACTTTCAG ACCACCAACT
    CGAGAAGACC CCCCTCAGAC TTTCTATCCG CAGAAAAGTT TCCCAGACAA AGCTCCAGTT
    AACGGAGCTG AGCAGACTCA GAAAACCATC ACTCCGGTGT ACAATCGATT CACACCAAAG
    CCGTACACAA GTTCTGCCCG GCCATTTGAA CGCAAATTTG AAAGTCCGAA GTTCAACCAT
    AATCTTCTGC CAAGTGAAAC TGTACATAAA CCTGAATTGT CTTCAAAAAC TCCCACTTCC
    CCAAAAACTC TTATGAAAGC TCATAGTTCA ACACAGCCTC CAGAGTTTGA CAGTGGAGTT
    GAAACTTTCT CTGTTCACAC AGATAAGCCT AAATATCAAA TGAATAATAT CAGCACCATG
    CCTAAAGCTG TCCCTGTGAG TCCTTCAGCT GTGGAAGAAG ATGAAGATGA GGATGGTCAT
    ACTGTAGTGG CTACAGCTCG TGGCATTTTT AACAGCAATG GTGGTGTGTT GAGTTCCATA
    GAAACCGGTG TTAGTATAAT TATCCCACAA GGAGCCATTC CTGAAGGAAT TGAGCAAGAA
    ATCTATTTCA AAGTCTGCAG AGACAATAGC ATTCTCCCAC CTTTAGATAA AGAGAAAGGT
    GAAACTCTGC TGAGCCCCCT AGTGATGTGT GGGCCCCATG GCCTCAAGTT CCTGAAGCCC
    GTGGAGCTAC GCTTGCCACA CTGTGCGTCC ATGACTCCTG ACGGTTGGTC TTTTGCTCTA
    AAATCATCCG ACTCCTCGTC GGGTGACCCT AAAACCTGGC AAAACAAGTG TCTTCCTGGA
    GATCCGAATT ACCTTGTTGG AGCCAACTGT GTTTCTGTCC TGATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006540843.1
    CDS72..5648
    Translation

    Target ORF information:

    RefSeq Version XM_006540780.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus tight junction protein 1 (Tjp1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006540780.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT
    CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA
    CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC
    AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA
    CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA
    AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT
    GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG
    AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG
    TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC
    ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT
    GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT
    GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA
    CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC
    TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT
    GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA
    CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG
    CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT
    AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG
    GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT
    GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT
    CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC
    CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT
    CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT
    GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT
    ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG
    TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA
    ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA
    ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT
    ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC
    ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT
    GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC
    CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT
    CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT
    CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA
    GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT
    AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG
    ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA
    ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT
    CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCCTCATCT
    CCAGTCCCTT ACCTTTCGCC TGAAACAACC CCAGCATCAT CAGCCTCTGC AGTTAATCAT
    AATGTCAGTG TAACTAATGT CAGCCTGGAG GAGCCTGCCC CAGCCCCTCC CACCTCGCAC
    GCATCACAGC CTGGTTGTTT AGGAGCACCA AGTGCTGAGG CAGCTCACGT AGTGCTCAGA
    GGTGAAGGAC CACCATTGCC GCCGCATGCA GACCCAGCAA AGGTGTACAG GAAGGAGCCG
    TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA
    GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA
    AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA
    GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT
    GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT
    CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT
    TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG
    CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC
    AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC
    AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC
    TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT
    TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA
    ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC
    AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT
    GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT
    AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG
    CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT
    GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG
    AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT
    TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAGG GAAAACCCGA AACTGATGCT
    GTGGATAGAT CATTCAGTGA GAAACGTTAT GATCCAGCCC AGGCCACGCC TCCTCCTCCT
    CCGTTGCCCT CACAGTACAG CCAGCCAGCT CCACCTCTGT CCAGCTCTTC TCTCCACATA
    CATTCCAAGG GCGCCCAGGG TGAAGGCAAC TCAGTATCAT TGGATTTTCA GAACTCATAT
    ATGTCCAAAC CAGACCCACC CCCATCTCAG AGTAAACCAG CAACTTTCAG ACCACCAACT
    CGAGAAGACC CCCCTCAGAC TTTCTATCCG CAGAAAAGTT TCCCAGACAA AGCTCCAGTT
    AACGGAGCTG AGCAGACTCA GAAAACCATC ACTCCGGTGT ACAATCGATT CACACCAAAG
    CCGTACACAA GTTCTGCCCG GCCATTTGAA CGCAAATTTG AAAGTCCGAA GTTCAACCAT
    AATCTTCTGC CAAGTGAAAC TGTACATAAA CCTGAATTGT CTTCAAAAAC TCCCACTTCC
    CCAAAAACTC TTATGAAAGC TCATAGTTCA ACACAGCCTC CAGAGTTTGA CAGTGGAGTT
    GAAACTTTCT CTGTTCACAC AGATAAGCCT AAATATCAAA TGAATAATAT CAGCACCATG
    CCTAAAGCTG TCCCTGTGAG TCCTTCAGCT GTGGAAGAAG ATGAAGATGA GGATGGTCAT
    ACTGTAGTGG CTACAGCTCG TGGCATTTTT AACAGCAATG GTGGTGTGTT GAGTTCCATA
    GAAACCGGTG TTAGTATAAT TATCCCACAA GGAGCCATTC CTGAAGGAAT TGAGCAAGAA
    ATCTATTTCA AAGTCTGCAG AGACAATAGC ATTCTCCCAC CTTTAGATAA AGAGAAAGGT
    GAAACTCTGC TGAGCCCCCT AGTGATGTGT GGGCCCCATG GCCTCAAGTT CCTGAAGCCC
    GTGGAGCTAC GCTTGCCACA CTGTGCGTCC ATGACTCCTG ACGGTTGGTC TTTTGCTCTA
    AAATCATCCG ACTCCTCGTC GGGTGACCCT AAAACCTGGC AAAACAAGTG TCTTCCTGGA
    GATCCGAATT ACCTTGTTGG AGCCAACTGT GTTTCTGTCC TGATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu38771
    Clone ID Related Accession (Same CDS sequence) XM_006540781.3 , XM_006540781.4
    Accession Version XM_006540781.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5568bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product tight junction protein ZO-1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006540781.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT
    CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA
    CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC
    AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA
    CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA
    AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT
    GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG
    AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG
    TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC
    ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT
    GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT
    GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA
    CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC
    TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT
    GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA
    CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG
    CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT
    AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG
    GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT
    GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT
    CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC
    CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT
    CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT
    GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT
    ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG
    TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA
    ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA
    ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT
    ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC
    ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT
    GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC
    CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT
    CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT
    CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA
    GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT
    AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG
    ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA
    ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT
    CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCCTCATCT
    CCAGTCCCTT ACCTTTCGCC TGAAACAACC CCAGCATCAT CAGCCTCTGC AGTTAATCAT
    AATGTCAGTG TAACTAATGT CAGCCTGGAG GAGCCTGCCC CAGCCCCTCC CACCTCGCAC
    GCATCACAGC CTGGTTGTTT AGGAGCACCA AGTGCTGAGG CAGCTCACGT AGTGCTCAGA
    GGTGAAGGAC CACCATTGCC GCCGCATGCA GACCCAGCAA AGGTGTACAG GAAGGAGCCG
    TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA
    GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA
    AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA
    GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT
    GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT
    CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT
    TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG
    CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC
    AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC
    AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC
    TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT
    TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA
    ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC
    AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT
    GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT
    AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG
    CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT
    GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG
    AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT
    TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAAT TTCTTGGCTC TTACACTAGC
    TATGACTACT TGAAAAGGAA CATCAAGTGG GGAAAACCCG AAACTGATGC TGTGGATAGA
    TCATTCAGTG AGAAACGTTA TGATCCAGCC CAGGCCACGC CTCCTCCTCC TCCGTTGCCC
    TCACAGTACA GCCAGCCAGC TCCACCTCTG TCCAGCTCTT CTCTCCACAT ACATTCCAAG
    GGCGCCCAGG GTGAAGGCAA CTCAGTATCA TTGGATTTTC AGAACTCATA TATGTCCAAA
    CCAGACCCAC CCCCATCTCA GAGTAAACCA GCAACTTTCA GACCACCAAC TCGAGAAGAC
    CCCCCTCAGA CTTTCTATCC GCAGAAAAGT TTCCCAGACA AAGCTCCAGT TAACGGAGCT
    GAGCAGACTC AGAAAACCAT CACTCCGGTG TACAATCGAT TCACACCAAA GCCGTACACA
    AGTTCTGCCC GGCCATTTGA ACGCAAATTT GAAAGTCCGA AGTTCAACCA TAATCTTCTG
    CCAAGTGAAA CTGTACATAA ACCTGAATTG TCTTCAAAAA CTCCCACTTC CCCAAAAACT
    CTTATGAAAG CTCATAGTTC AACACAGCCT CCAGAGTTTG ACAGTGGAGT TGAAACTTTC
    TCTGTTCACA CAGATAAGCC TAAATATCAA ATGAATAATA TCAGCACCAT GCCTAAAGCT
    GTCCCTGTGA GTCCTTCAGC TGTGGAAGAA GATGAAGATG AGGATGGTCA TACTGTAGTG
    GCTACAGCTC GTGGCATTTT TAACAGCAAT GGTGGTGTGT TGAGTTCCAT AGAAACCGGT
    GTTAGTATAA TTATCCCACA AGGAGCCATT CCTGAAGGAA TTGAGCAAGA AATCTATTTC
    AAAGTCTGCA GAGACAATAG CATTCTCCCA CCTTTAGATA AAGAGAAAGG TGAAACTCTG
    CTGAGCCCCC TAGTGATGTG TGGGCCCCAT GGCCTCAAGT TCCTGAAGCC CGTGGAGCTA
    CGCTTGCCAC ACTGTGACCC TAAAACCTGG CAAAACAAGT GTCTTCCTGG AGATCCGAAT
    TACCTTGTTG GAGCCAACTG TGTTTCTGTC CTGATTGACC ACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006540844.1
    CDS73..5640
    Translation

    Target ORF information:

    RefSeq Version XM_006540781.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus tight junction protein 1 (Tjp1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006540781.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT
    CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA
    CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC
    AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA
    CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA
    AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT
    GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG
    AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG
    TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC
    ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT
    GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT
    GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA
    CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC
    TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT
    GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA
    CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG
    CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT
    AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG
    GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT
    GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT
    CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC
    CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT
    CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT
    GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT
    ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG
    TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA
    ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA
    ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT
    ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC
    ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT
    GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC
    CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT
    CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT
    CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA
    GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT
    AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG
    ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA
    ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT
    CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCCTCATCT
    CCAGTCCCTT ACCTTTCGCC TGAAACAACC CCAGCATCAT CAGCCTCTGC AGTTAATCAT
    AATGTCAGTG TAACTAATGT CAGCCTGGAG GAGCCTGCCC CAGCCCCTCC CACCTCGCAC
    GCATCACAGC CTGGTTGTTT AGGAGCACCA AGTGCTGAGG CAGCTCACGT AGTGCTCAGA
    GGTGAAGGAC CACCATTGCC GCCGCATGCA GACCCAGCAA AGGTGTACAG GAAGGAGCCG
    TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA
    GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA
    AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA
    GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT
    GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT
    CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT
    TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG
    CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC
    AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC
    AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC
    TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT
    TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA
    ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC
    AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT
    GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT
    AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG
    CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT
    GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG
    AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT
    TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAAT TTCTTGGCTC TTACACTAGC
    TATGACTACT TGAAAAGGAA CATCAAGTGG GGAAAACCCG AAACTGATGC TGTGGATAGA
    TCATTCAGTG AGAAACGTTA TGATCCAGCC CAGGCCACGC CTCCTCCTCC TCCGTTGCCC
    TCACAGTACA GCCAGCCAGC TCCACCTCTG TCCAGCTCTT CTCTCCACAT ACATTCCAAG
    GGCGCCCAGG GTGAAGGCAA CTCAGTATCA TTGGATTTTC AGAACTCATA TATGTCCAAA
    CCAGACCCAC CCCCATCTCA GAGTAAACCA GCAACTTTCA GACCACCAAC TCGAGAAGAC
    CCCCCTCAGA CTTTCTATCC GCAGAAAAGT TTCCCAGACA AAGCTCCAGT TAACGGAGCT
    GAGCAGACTC AGAAAACCAT CACTCCGGTG TACAATCGAT TCACACCAAA GCCGTACACA
    AGTTCTGCCC GGCCATTTGA ACGCAAATTT GAAAGTCCGA AGTTCAACCA TAATCTTCTG
    CCAAGTGAAA CTGTACATAA ACCTGAATTG TCTTCAAAAA CTCCCACTTC CCCAAAAACT
    CTTATGAAAG CTCATAGTTC AACACAGCCT CCAGAGTTTG ACAGTGGAGT TGAAACTTTC
    TCTGTTCACA CAGATAAGCC TAAATATCAA ATGAATAATA TCAGCACCAT GCCTAAAGCT
    GTCCCTGTGA GTCCTTCAGC TGTGGAAGAA GATGAAGATG AGGATGGTCA TACTGTAGTG
    GCTACAGCTC GTGGCATTTT TAACAGCAAT GGTGGTGTGT TGAGTTCCAT AGAAACCGGT
    GTTAGTATAA TTATCCCACA AGGAGCCATT CCTGAAGGAA TTGAGCAAGA AATCTATTTC
    AAAGTCTGCA GAGACAATAG CATTCTCCCA CCTTTAGATA AAGAGAAAGG TGAAACTCTG
    CTGAGCCCCC TAGTGATGTG TGGGCCCCAT GGCCTCAAGT TCCTGAAGCC CGTGGAGCTA
    CGCTTGCCAC ACTGTGACCC TAAAACCTGG CAAAACAAGT GTCTTCCTGG AGATCCGAAT
    TACCTTGTTG GAGCCAACTG TGTTTCTGTC CTGATTGACC ACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu38772
    Clone ID Related Accession (Same CDS sequence) XM_006540782.4 , XM_006540782.3
    Accession Version XM_006540782.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5517bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product tight junction protein ZO-1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006540782.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT
    CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA
    CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC
    AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA
    CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA
    AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT
    GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG
    AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG
    TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC
    ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT
    GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT
    GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA
    CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC
    TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT
    GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA
    CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG
    CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT
    AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG
    GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT
    GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT
    CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC
    CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT
    CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT
    GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT
    ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG
    TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA
    ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA
    ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT
    ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC
    ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT
    GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC
    CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT
    CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT
    CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA
    GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT
    AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG
    ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA
    ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT
    CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCCTCATCT
    CCAGTCCCTT ACCTTTCGCC TGAAACAACC CCAGCATCAT CAGCCTCTGC AGTTAATCAT
    AATGTCAGTG TAACTAATGT CAGCCTGGAG GAGCCTGCCC CAGCCCCTCC CACCTCGCAC
    GCATCACAGC CTGGTTGTTT AGGAGCACCA AGTGCTGAGG CAGCTCACGT AGTGCTCAGA
    GGTGAAGGAC CACCATTGCC GCCGCATGCA GACCCAGCAA AGGTGTACAG GAAGGAGCCG
    TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA
    GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA
    AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA
    GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT
    GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT
    CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT
    TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG
    CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC
    AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC
    AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC
    TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT
    TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA
    ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC
    AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT
    GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT
    AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG
    CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT
    GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG
    AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT
    TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAGG GAAAACCCGA AACTGATGCT
    GTGGATAGAT CATTCAGTGA GAAACGTTAT GATCCAGCCC AGGCCACGCC TCCTCCTCCT
    CCGTTGCCCT CACAGTACAG CCAGCCAGCT CCACCTCTGT CCAGCTCTTC TCTCCACATA
    CATTCCAAGG GCGCCCAGGG TGAAGGCAAC TCAGTATCAT TGGATTTTCA GAACTCATAT
    ATGTCCAAAC CAGACCCACC CCCATCTCAG AGTAAACCAG CAACTTTCAG ACCACCAACT
    CGAGAAGACC CCCCTCAGAC TTTCTATCCG CAGAAAAGTT TCCCAGACAA AGCTCCAGTT
    AACGGAGCTG AGCAGACTCA GAAAACCATC ACTCCGGTGT ACAATCGATT CACACCAAAG
    CCGTACACAA GTTCTGCCCG GCCATTTGAA CGCAAATTTG AAAGTCCGAA GTTCAACCAT
    AATCTTCTGC CAAGTGAAAC TGTACATAAA CCTGAATTGT CTTCAAAAAC TCCCACTTCC
    CCAAAAACTC TTATGAAAGC TCATAGTTCA ACACAGCCTC CAGAGTTTGA CAGTGGAGTT
    GAAACTTTCT CTGTTCACAC AGATAAGCCT AAATATCAAA TGAATAATAT CAGCACCATG
    CCTAAAGCTG TCCCTGTGAG TCCTTCAGCT GTGGAAGAAG ATGAAGATGA GGATGGTCAT
    ACTGTAGTGG CTACAGCTCG TGGCATTTTT AACAGCAATG GTGGTGTGTT GAGTTCCATA
    GAAACCGGTG TTAGTATAAT TATCCCACAA GGAGCCATTC CTGAAGGAAT TGAGCAAGAA
    ATCTATTTCA AAGTCTGCAG AGACAATAGC ATTCTCCCAC CTTTAGATAA AGAGAAAGGT
    GAAACTCTGC TGAGCCCCCT AGTGATGTGT GGGCCCCATG GCCTCAAGTT CCTGAAGCCC
    GTGGAGCTAC GCTTGCCACA CTGTGACCCT AAAACCTGGC AAAACAAGTG TCTTCCTGGA
    GATCCGAATT ACCTTGTTGG AGCCAACTGT GTTTCTGTCC TGATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006540845.1
    CDS72..5588
    Translation

    Target ORF information:

    RefSeq Version XM_006540782.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus tight junction protein 1 (Tjp1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006540782.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT
    CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA
    CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC
    AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA
    CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA
    AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT
    GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG
    AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG
    TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC
    ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT
    GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT
    GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA
    CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC
    TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT
    GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA
    CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG
    CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT
    AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG
    GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT
    GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT
    CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC
    CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT
    CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT
    GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT
    ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG
    TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA
    ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA
    ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT
    ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC
    ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT
    GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC
    CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT
    CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT
    CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA
    GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT
    AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG
    ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA
    ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT
    CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCCTCATCT
    CCAGTCCCTT ACCTTTCGCC TGAAACAACC CCAGCATCAT CAGCCTCTGC AGTTAATCAT
    AATGTCAGTG TAACTAATGT CAGCCTGGAG GAGCCTGCCC CAGCCCCTCC CACCTCGCAC
    GCATCACAGC CTGGTTGTTT AGGAGCACCA AGTGCTGAGG CAGCTCACGT AGTGCTCAGA
    GGTGAAGGAC CACCATTGCC GCCGCATGCA GACCCAGCAA AGGTGTACAG GAAGGAGCCG
    TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA
    GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA
    AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA
    GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT
    GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT
    CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT
    TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG
    CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC
    AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC
    AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC
    TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT
    TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA
    ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC
    AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT
    GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT
    AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG
    CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT
    GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG
    AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT
    TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAGG GAAAACCCGA AACTGATGCT
    GTGGATAGAT CATTCAGTGA GAAACGTTAT GATCCAGCCC AGGCCACGCC TCCTCCTCCT
    CCGTTGCCCT CACAGTACAG CCAGCCAGCT CCACCTCTGT CCAGCTCTTC TCTCCACATA
    CATTCCAAGG GCGCCCAGGG TGAAGGCAAC TCAGTATCAT TGGATTTTCA GAACTCATAT
    ATGTCCAAAC CAGACCCACC CCCATCTCAG AGTAAACCAG CAACTTTCAG ACCACCAACT
    CGAGAAGACC CCCCTCAGAC TTTCTATCCG CAGAAAAGTT TCCCAGACAA AGCTCCAGTT
    AACGGAGCTG AGCAGACTCA GAAAACCATC ACTCCGGTGT ACAATCGATT CACACCAAAG
    CCGTACACAA GTTCTGCCCG GCCATTTGAA CGCAAATTTG AAAGTCCGAA GTTCAACCAT
    AATCTTCTGC CAAGTGAAAC TGTACATAAA CCTGAATTGT CTTCAAAAAC TCCCACTTCC
    CCAAAAACTC TTATGAAAGC TCATAGTTCA ACACAGCCTC CAGAGTTTGA CAGTGGAGTT
    GAAACTTTCT CTGTTCACAC AGATAAGCCT AAATATCAAA TGAATAATAT CAGCACCATG
    CCTAAAGCTG TCCCTGTGAG TCCTTCAGCT GTGGAAGAAG ATGAAGATGA GGATGGTCAT
    ACTGTAGTGG CTACAGCTCG TGGCATTTTT AACAGCAATG GTGGTGTGTT GAGTTCCATA
    GAAACCGGTG TTAGTATAAT TATCCCACAA GGAGCCATTC CTGAAGGAAT TGAGCAAGAA
    ATCTATTTCA AAGTCTGCAG AGACAATAGC ATTCTCCCAC CTTTAGATAA AGAGAAAGGT
    GAAACTCTGC TGAGCCCCCT AGTGATGTGT GGGCCCCATG GCCTCAAGTT CCTGAAGCCC
    GTGGAGCTAC GCTTGCCACA CTGTGACCCT AAAACCTGGC AAAACAAGTG TCTTCCTGGA
    GATCCGAATT ACCTTGTTGG AGCCAACTGT GTTTCTGTCC TGATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu38773
    Clone ID Related Accession (Same CDS sequence) XM_006540783.4 , XM_006540783.3
    Accession Version XM_006540783.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5388bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product tight junction protein ZO-1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006540783.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT
    CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA
    CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC
    AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA
    CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA
    AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT
    GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG
    AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG
    TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC
    ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT
    GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT
    GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA
    CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC
    TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT
    GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA
    CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG
    CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT
    AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG
    GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT
    GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT
    CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC
    CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT
    CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT
    GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT
    ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG
    TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA
    ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA
    ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT
    ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC
    ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT
    GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC
    CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT
    CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT
    CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA
    GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT
    AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG
    ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA
    ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT
    CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGGTGTACAG GAAGGAGCCG
    TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA
    GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA
    AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA
    GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT
    GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT
    CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT
    TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG
    CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC
    AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC
    AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC
    TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT
    TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA
    ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC
    AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT
    GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT
    AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG
    CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT
    GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG
    AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT
    TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAAT TTCTTGGCTC TTACACTAGC
    TATGACTACT TGAAAAGGAA CATCAAGTGG GGAAAACCCG AAACTGATGC TGTGGATAGA
    TCATTCAGTG AGAAACGTTA TGATCCAGCC CAGGCCACGC CTCCTCCTCC TCCGTTGCCC
    TCACAGTACA GCCAGCCAGC TCCACCTCTG TCCAGCTCTT CTCTCCACAT ACATTCCAAG
    GGCGCCCAGG GTGAAGGCAA CTCAGTATCA TTGGATTTTC AGAACTCATA TATGTCCAAA
    CCAGACCCAC CCCCATCTCA GAGTAAACCA GCAACTTTCA GACCACCAAC TCGAGAAGAC
    CCCCCTCAGA CTTTCTATCC GCAGAAAAGT TTCCCAGACA AAGCTCCAGT TAACGGAGCT
    GAGCAGACTC AGAAAACCAT CACTCCGGTG TACAATCGAT TCACACCAAA GCCGTACACA
    AGTTCTGCCC GGCCATTTGA ACGCAAATTT GAAAGTCCGA AGTTCAACCA TAATCTTCTG
    CCAAGTGAAA CTGTACATAA ACCTGAATTG TCTTCAAAAA CTCCCACTTC CCCAAAAACT
    CTTATGAAAG CTCATAGTTC AACACAGCCT CCAGAGTTTG ACAGTGGAGT TGAAACTTTC
    TCTGTTCACA CAGATAAGCC TAAATATCAA ATGAATAATA TCAGCACCAT GCCTAAAGCT
    GTCCCTGTGA GTCCTTCAGC TGTGGAAGAA GATGAAGATG AGGATGGTCA TACTGTAGTG
    GCTACAGCTC GTGGCATTTT TAACAGCAAT GGTGGTGTGT TGAGTTCCAT AGAAACCGGT
    GTTAGTATAA TTATCCCACA AGGAGCCATT CCTGAAGGAA TTGAGCAAGA AATCTATTTC
    AAAGTCTGCA GAGACAATAG CATTCTCCCA CCTTTAGATA AAGAGAAAGG TGAAACTCTG
    CTGAGCCCCC TAGTGATGTG TGGGCCCCAT GGCCTCAAGT TCCTGAAGCC CGTGGAGCTA
    CGCTTGCCAC ACTGTGCGTC CATGACTCCT GACGGTTGGT CTTTTGCTCT AAAATCATCC
    GACTCCTCGT CGGGTGACCC TAAAACCTGG CAAAACAAGT GTCTTCCTGG AGATCCGAAT
    TACCTTGTTG GAGCCAACTG TGTTTCTGTC CTGATTGACC ACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006540846.1
    CDS72..5459
    Translation

    Target ORF information:

    RefSeq Version XM_006540783.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus tight junction protein 1 (Tjp1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006540783.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT
    CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA
    CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC
    AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA
    CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA
    AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT
    GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG
    AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG
    TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC
    ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT
    GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT
    GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA
    CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC
    TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT
    GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA
    CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG
    CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT
    AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG
    GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT
    GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT
    CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC
    CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT
    CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT
    GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT
    ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG
    TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA
    ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA
    ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT
    ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC
    ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT
    GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC
    CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT
    CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT
    CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA
    GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT
    AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG
    ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA
    ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT
    CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGGTGTACAG GAAGGAGCCG
    TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA
    GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA
    AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA
    GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT
    GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT
    CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT
    TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG
    CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC
    AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC
    AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC
    TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT
    TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA
    ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC
    AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT
    GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT
    AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG
    CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT
    GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG
    AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT
    TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAAT TTCTTGGCTC TTACACTAGC
    TATGACTACT TGAAAAGGAA CATCAAGTGG GGAAAACCCG AAACTGATGC TGTGGATAGA
    TCATTCAGTG AGAAACGTTA TGATCCAGCC CAGGCCACGC CTCCTCCTCC TCCGTTGCCC
    TCACAGTACA GCCAGCCAGC TCCACCTCTG TCCAGCTCTT CTCTCCACAT ACATTCCAAG
    GGCGCCCAGG GTGAAGGCAA CTCAGTATCA TTGGATTTTC AGAACTCATA TATGTCCAAA
    CCAGACCCAC CCCCATCTCA GAGTAAACCA GCAACTTTCA GACCACCAAC TCGAGAAGAC
    CCCCCTCAGA CTTTCTATCC GCAGAAAAGT TTCCCAGACA AAGCTCCAGT TAACGGAGCT
    GAGCAGACTC AGAAAACCAT CACTCCGGTG TACAATCGAT TCACACCAAA GCCGTACACA
    AGTTCTGCCC GGCCATTTGA ACGCAAATTT GAAAGTCCGA AGTTCAACCA TAATCTTCTG
    CCAAGTGAAA CTGTACATAA ACCTGAATTG TCTTCAAAAA CTCCCACTTC CCCAAAAACT
    CTTATGAAAG CTCATAGTTC AACACAGCCT CCAGAGTTTG ACAGTGGAGT TGAAACTTTC
    TCTGTTCACA CAGATAAGCC TAAATATCAA ATGAATAATA TCAGCACCAT GCCTAAAGCT
    GTCCCTGTGA GTCCTTCAGC TGTGGAAGAA GATGAAGATG AGGATGGTCA TACTGTAGTG
    GCTACAGCTC GTGGCATTTT TAACAGCAAT GGTGGTGTGT TGAGTTCCAT AGAAACCGGT
    GTTAGTATAA TTATCCCACA AGGAGCCATT CCTGAAGGAA TTGAGCAAGA AATCTATTTC
    AAAGTCTGCA GAGACAATAG CATTCTCCCA CCTTTAGATA AAGAGAAAGG TGAAACTCTG
    CTGAGCCCCC TAGTGATGTG TGGGCCCCAT GGCCTCAAGT TCCTGAAGCC CGTGGAGCTA
    CGCTTGCCAC ACTGTGCGTC CATGACTCCT GACGGTTGGT CTTTTGCTCT AAAATCATCC
    GACTCCTCGT CGGGTGACCC TAAAACCTGG CAAAACAAGT GTCTTCCTGG AGATCCGAAT
    TACCTTGTTG GAGCCAACTG TGTTTCTGTC CTGATTGACC ACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu38775
    Clone ID Related Accession (Same CDS sequence) XM_006540785.3 , XM_006540785.4
    Accession Version XM_006540785.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5337bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product tight junction protein ZO-1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006540785.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT
    CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA
    CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC
    AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA
    CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA
    AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT
    GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG
    AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG
    TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC
    ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT
    GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT
    GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA
    CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC
    TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT
    GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA
    CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG
    CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT
    AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG
    GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT
    GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT
    CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC
    CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT
    CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT
    GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT
    ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG
    TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA
    ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA
    ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT
    ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC
    ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT
    GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC
    CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT
    CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT
    CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA
    GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT
    AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG
    ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA
    ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT
    CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGGTGTACAG GAAGGAGCCG
    TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA
    GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA
    AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA
    GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT
    GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT
    CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT
    TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG
    CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC
    AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC
    AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC
    TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT
    TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA
    ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC
    AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT
    GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT
    AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG
    CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT
    GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG
    AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT
    TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAGG GAAAACCCGA AACTGATGCT
    GTGGATAGAT CATTCAGTGA GAAACGTTAT GATCCAGCCC AGGCCACGCC TCCTCCTCCT
    CCGTTGCCCT CACAGTACAG CCAGCCAGCT CCACCTCTGT CCAGCTCTTC TCTCCACATA
    CATTCCAAGG GCGCCCAGGG TGAAGGCAAC TCAGTATCAT TGGATTTTCA GAACTCATAT
    ATGTCCAAAC CAGACCCACC CCCATCTCAG AGTAAACCAG CAACTTTCAG ACCACCAACT
    CGAGAAGACC CCCCTCAGAC TTTCTATCCG CAGAAAAGTT TCCCAGACAA AGCTCCAGTT
    AACGGAGCTG AGCAGACTCA GAAAACCATC ACTCCGGTGT ACAATCGATT CACACCAAAG
    CCGTACACAA GTTCTGCCCG GCCATTTGAA CGCAAATTTG AAAGTCCGAA GTTCAACCAT
    AATCTTCTGC CAAGTGAAAC TGTACATAAA CCTGAATTGT CTTCAAAAAC TCCCACTTCC
    CCAAAAACTC TTATGAAAGC TCATAGTTCA ACACAGCCTC CAGAGTTTGA CAGTGGAGTT
    GAAACTTTCT CTGTTCACAC AGATAAGCCT AAATATCAAA TGAATAATAT CAGCACCATG
    CCTAAAGCTG TCCCTGTGAG TCCTTCAGCT GTGGAAGAAG ATGAAGATGA GGATGGTCAT
    ACTGTAGTGG CTACAGCTCG TGGCATTTTT AACAGCAATG GTGGTGTGTT GAGTTCCATA
    GAAACCGGTG TTAGTATAAT TATCCCACAA GGAGCCATTC CTGAAGGAAT TGAGCAAGAA
    ATCTATTTCA AAGTCTGCAG AGACAATAGC ATTCTCCCAC CTTTAGATAA AGAGAAAGGT
    GAAACTCTGC TGAGCCCCCT AGTGATGTGT GGGCCCCATG GCCTCAAGTT CCTGAAGCCC
    GTGGAGCTAC GCTTGCCACA CTGTGCGTCC ATGACTCCTG ACGGTTGGTC TTTTGCTCTA
    AAATCATCCG ACTCCTCGTC GGGTGACCCT AAAACCTGGC AAAACAAGTG TCTTCCTGGA
    GATCCGAATT ACCTTGTTGG AGCCAACTGT GTTTCTGTCC TGATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006540848.1
    CDS72..5408
    Translation

    Target ORF information:

    RefSeq Version XM_006540785.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus tight junction protein 1 (Tjp1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006540785.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT
    CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA
    CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC
    AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA
    CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA
    AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT
    GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG
    AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG
    TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC
    ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT
    GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT
    GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA
    CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC
    TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT
    GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA
    CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG
    CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT
    AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG
    GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT
    GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT
    CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC
    CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT
    CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT
    GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT
    ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG
    TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA
    ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA
    ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT
    ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC
    ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT
    GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC
    CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT
    CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT
    CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA
    GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT
    AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG
    ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA
    ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT
    CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGGTGTACAG GAAGGAGCCG
    TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA
    GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA
    AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA
    GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT
    GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT
    CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT
    TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG
    CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC
    AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC
    AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC
    TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT
    TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA
    ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC
    AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT
    GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT
    AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG
    CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT
    GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG
    AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT
    TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAGG GAAAACCCGA AACTGATGCT
    GTGGATAGAT CATTCAGTGA GAAACGTTAT GATCCAGCCC AGGCCACGCC TCCTCCTCCT
    CCGTTGCCCT CACAGTACAG CCAGCCAGCT CCACCTCTGT CCAGCTCTTC TCTCCACATA
    CATTCCAAGG GCGCCCAGGG TGAAGGCAAC TCAGTATCAT TGGATTTTCA GAACTCATAT
    ATGTCCAAAC CAGACCCACC CCCATCTCAG AGTAAACCAG CAACTTTCAG ACCACCAACT
    CGAGAAGACC CCCCTCAGAC TTTCTATCCG CAGAAAAGTT TCCCAGACAA AGCTCCAGTT
    AACGGAGCTG AGCAGACTCA GAAAACCATC ACTCCGGTGT ACAATCGATT CACACCAAAG
    CCGTACACAA GTTCTGCCCG GCCATTTGAA CGCAAATTTG AAAGTCCGAA GTTCAACCAT
    AATCTTCTGC CAAGTGAAAC TGTACATAAA CCTGAATTGT CTTCAAAAAC TCCCACTTCC
    CCAAAAACTC TTATGAAAGC TCATAGTTCA ACACAGCCTC CAGAGTTTGA CAGTGGAGTT
    GAAACTTTCT CTGTTCACAC AGATAAGCCT AAATATCAAA TGAATAATAT CAGCACCATG
    CCTAAAGCTG TCCCTGTGAG TCCTTCAGCT GTGGAAGAAG ATGAAGATGA GGATGGTCAT
    ACTGTAGTGG CTACAGCTCG TGGCATTTTT AACAGCAATG GTGGTGTGTT GAGTTCCATA
    GAAACCGGTG TTAGTATAAT TATCCCACAA GGAGCCATTC CTGAAGGAAT TGAGCAAGAA
    ATCTATTTCA AAGTCTGCAG AGACAATAGC ATTCTCCCAC CTTTAGATAA AGAGAAAGGT
    GAAACTCTGC TGAGCCCCCT AGTGATGTGT GGGCCCCATG GCCTCAAGTT CCTGAAGCCC
    GTGGAGCTAC GCTTGCCACA CTGTGCGTCC ATGACTCCTG ACGGTTGGTC TTTTGCTCTA
    AAATCATCCG ACTCCTCGTC GGGTGACCCT AAAACCTGGC AAAACAAGTG TCTTCCTGGA
    GATCCGAATT ACCTTGTTGG AGCCAACTGT GTTTCTGTCC TGATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu38776
    Clone ID Related Accession (Same CDS sequence) XM_006540786.4 , XM_006540786.3
    Accession Version XM_006540786.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5277bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product tight junction protein ZO-1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006540786.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT
    CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA
    CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC
    AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA
    CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA
    AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT
    GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG
    AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG
    TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC
    ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT
    GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT
    GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA
    CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC
    TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT
    GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA
    CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG
    CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT
    AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG
    GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT
    GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT
    CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC
    CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT
    CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT
    GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT
    ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG
    TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA
    ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA
    ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT
    ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC
    ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT
    GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC
    CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT
    CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT
    CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA
    GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT
    AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG
    ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA
    ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT
    CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGGTGTACAG GAAGGAGCCG
    TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA
    GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA
    AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA
    GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT
    GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT
    CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT
    TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG
    CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC
    AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC
    AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC
    TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT
    TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA
    ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC
    AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT
    GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT
    AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG
    CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT
    GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG
    AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT
    TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAGG GAAAACCCGA AACTGATGCT
    GTGGATAGAT CATTCAGTGA GAAACGTTAT GATCCAGCCC AGGCCACGCC TCCTCCTCCT
    CCGTTGCCCT CACAGTACAG CCAGCCAGCT CCACCTCTGT CCAGCTCTTC TCTCCACATA
    CATTCCAAGG GCGCCCAGGG TGAAGGCAAC TCAGTATCAT TGGATTTTCA GAACTCATAT
    ATGTCCAAAC CAGACCCACC CCCATCTCAG AGTAAACCAG CAACTTTCAG ACCACCAACT
    CGAGAAGACC CCCCTCAGAC TTTCTATCCG CAGAAAAGTT TCCCAGACAA AGCTCCAGTT
    AACGGAGCTG AGCAGACTCA GAAAACCATC ACTCCGGTGT ACAATCGATT CACACCAAAG
    CCGTACACAA GTTCTGCCCG GCCATTTGAA CGCAAATTTG AAAGTCCGAA GTTCAACCAT
    AATCTTCTGC CAAGTGAAAC TGTACATAAA CCTGAATTGT CTTCAAAAAC TCCCACTTCC
    CCAAAAACTC TTATGAAAGC TCATAGTTCA ACACAGCCTC CAGAGTTTGA CAGTGGAGTT
    GAAACTTTCT CTGTTCACAC AGATAAGCCT AAATATCAAA TGAATAATAT CAGCACCATG
    CCTAAAGCTG TCCCTGTGAG TCCTTCAGCT GTGGAAGAAG ATGAAGATGA GGATGGTCAT
    ACTGTAGTGG CTACAGCTCG TGGCATTTTT AACAGCAATG GTGGTGTGTT GAGTTCCATA
    GAAACCGGTG TTAGTATAAT TATCCCACAA GGAGCCATTC CTGAAGGAAT TGAGCAAGAA
    ATCTATTTCA AAGTCTGCAG AGACAATAGC ATTCTCCCAC CTTTAGATAA AGAGAAAGGT
    GAAACTCTGC TGAGCCCCCT AGTGATGTGT GGGCCCCATG GCCTCAAGTT CCTGAAGCCC
    GTGGAGCTAC GCTTGCCACA CTGTGACCCT AAAACCTGGC AAAACAAGTG TCTTCCTGGA
    GATCCGAATT ACCTTGTTGG AGCCAACTGT GTTTCTGTCC TGATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006540849.1
    CDS72..5348
    Translation

    Target ORF information:

    RefSeq Version XM_006540786.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus tight junction protein 1 (Tjp1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006540786.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT
    CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA
    CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC
    AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA
    CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA
    AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT
    GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG
    AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG
    TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC
    ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT
    GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT
    GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA
    CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC
    TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT
    GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA
    CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG
    CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT
    AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG
    GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT
    GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT
    CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC
    CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT
    CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT
    GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT
    ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG
    TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA
    ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA
    ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT
    ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC
    ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT
    GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC
    CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT
    CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT
    CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA
    GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT
    AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG
    ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA
    ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT
    CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGGTGTACAG GAAGGAGCCG
    TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA
    GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA
    AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA
    GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT
    GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT
    CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT
    TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG
    CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC
    AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC
    AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC
    TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT
    TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA
    ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC
    AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT
    GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT
    AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG
    CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT
    GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG
    AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT
    TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAGG GAAAACCCGA AACTGATGCT
    GTGGATAGAT CATTCAGTGA GAAACGTTAT GATCCAGCCC AGGCCACGCC TCCTCCTCCT
    CCGTTGCCCT CACAGTACAG CCAGCCAGCT CCACCTCTGT CCAGCTCTTC TCTCCACATA
    CATTCCAAGG GCGCCCAGGG TGAAGGCAAC TCAGTATCAT TGGATTTTCA GAACTCATAT
    ATGTCCAAAC CAGACCCACC CCCATCTCAG AGTAAACCAG CAACTTTCAG ACCACCAACT
    CGAGAAGACC CCCCTCAGAC TTTCTATCCG CAGAAAAGTT TCCCAGACAA AGCTCCAGTT
    AACGGAGCTG AGCAGACTCA GAAAACCATC ACTCCGGTGT ACAATCGATT CACACCAAAG
    CCGTACACAA GTTCTGCCCG GCCATTTGAA CGCAAATTTG AAAGTCCGAA GTTCAACCAT
    AATCTTCTGC CAAGTGAAAC TGTACATAAA CCTGAATTGT CTTCAAAAAC TCCCACTTCC
    CCAAAAACTC TTATGAAAGC TCATAGTTCA ACACAGCCTC CAGAGTTTGA CAGTGGAGTT
    GAAACTTTCT CTGTTCACAC AGATAAGCCT AAATATCAAA TGAATAATAT CAGCACCATG
    CCTAAAGCTG TCCCTGTGAG TCCTTCAGCT GTGGAAGAAG ATGAAGATGA GGATGGTCAT
    ACTGTAGTGG CTACAGCTCG TGGCATTTTT AACAGCAATG GTGGTGTGTT GAGTTCCATA
    GAAACCGGTG TTAGTATAAT TATCCCACAA GGAGCCATTC CTGAAGGAAT TGAGCAAGAA
    ATCTATTTCA AAGTCTGCAG AGACAATAGC ATTCTCCCAC CTTTAGATAA AGAGAAAGGT
    GAAACTCTGC TGAGCCCCCT AGTGATGTGT GGGCCCCATG GCCTCAAGTT CCTGAAGCCC
    GTGGAGCTAC GCTTGCCACA CTGTGACCCT AAAACCTGGC AAAACAAGTG TCTTCCTGGA
    GATCCGAATT ACCTTGTTGG AGCCAACTGT GTTTCTGTCC TGATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu68895
    Clone ID Related Accession (Same CDS sequence) XM_011250843.2 , XM_011250843.3
    Accession Version XM_011250843.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5538bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product tight junction protein ZO-1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011250843.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT
    CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA
    CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC
    AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA
    CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA
    AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT
    GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG
    AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG
    TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC
    ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT
    GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT
    GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA
    CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC
    TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT
    GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA
    CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG
    CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT
    AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG
    GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT
    GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT
    CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC
    CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT
    CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT
    GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT
    ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG
    TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA
    ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA
    ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT
    ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC
    ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT
    GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC
    CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT
    CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT
    CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA
    GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT
    AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG
    ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA
    ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT
    CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCCTCATCT
    CCAGTCCCTT ACCTTTCGCC TGAAACAACC CCAGCATCAT CAGCCTCTGC AGTTAATCAT
    AATGTCAGTG TAACTAATGT CAGCCTGGAG GAGCCTGCCC CAGCCCCTCC CACCTCGCAC
    GCATCACAGC CTGGTTGTTT AGGAGCACCA AGTGCTGAGG CAGCTCACGT AGTGCTCAGA
    GGTGAAGGAC CACCATTGCC GCCGCATGCA GACCCAGCAA AGGTGTACAG GAAGGAGCCG
    TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA
    GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA
    AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA
    GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT
    GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT
    CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT
    TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG
    CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC
    AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC
    AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC
    TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT
    TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA
    ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC
    AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT
    GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT
    AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG
    CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT
    GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG
    AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT
    TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAGG GAAAACCCGA AACTGATGCT
    GTGGATAGAT CATTCAGTGA GAAACGTTAT GATCCAGCCC AGGCCACGCC TCCTCCTCCT
    CCGTTGCCCT CACAGTACAG CCAGCCAGCT CCACCTCTGT CCAGCTCTTC TCTCCACATA
    CATTCCAAGG GCGCCCAGGG TGAAGGCAAC TCAGTATCAT TGGATTTTCA GAACTCATAT
    ATGTCCAAAC CAGACCCACC CCCATCTCAG AGTAAACCAG CAACTTTCAG ACCACCAACT
    CGAGAAGACC CCCCTCAGAC TTTCTATCCG CAGAAAAGTT TCCCAGACAA AGCTCCAGTT
    AACGGAGCTG AGCAGACTCA GAAAACCATC ACTCCGGTGT ACAATCGATT CACACCAAAG
    CCGTACACAA GTTCTGCCCG GCCATTTGAA CGCAAATTTG AAAGTCCGAA GTTCAACCAT
    AATCTTCTGC CAAGTGAAAC TGTACATAAA CCTGAATTGT CTTCAAAAAC TCCCACTTCC
    CCAAAAACTC TTATGAAAGC TCATAGTTCA ACACAGCCTC CAGAGTTTGA CAGTGGAGTT
    GAAACTTTCT CTGTTCACAC AGATAAGCCT AAATATCAAA TGAATAATAT CAGCACCATG
    CCTAAAGCTG TCCCTGTGAG TCCTTCAGCT GTGGAAGAAG ATGAAGATGA GGATGGTCAT
    ACTGTAGTGG CTACAGCTCG TGGCATTTTT AACAGCAATG GTGGTGTGTT GAGTTCCATA
    GAAACCGGTG TTAGTATAAT TATCCCACAA GGAGCCATTC CTGAAGGAAT TGAGCAAGAA
    ATCTATTTCA AAGTCTGCAG AGACAATAGC ATTCTCCCAC CTTTAGATAA AGAGAAAGGT
    GAAACTCTGC TGAGCCCCCT AGTGATGTGT GGGCCCCATG GCCTCAAGTT CCTGAAGCCC
    GTGGAGCTAC GCTTGCCACA CTGTGCGTCC ATGACTCCTG ACGGTGACCC TAAAACCTGG
    CAAAACAAGT GTCTTCCTGG AGATCCGAAT TACCTTGTTG GAGCCAACTG TGTTTCTGTC
    CTGATTGACC ACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011249145.1
    CDS72..5609
    Translation

    Target ORF information:

    RefSeq Version XM_011250843.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus tight junction protein 1 (Tjp1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011250843.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACT
    CCATCCTCTG AAAACCCCCA TGGTGCTACT TCCACTGTCA GCCAAGGAAA ACCTTCTCTA
    CGACGAATTA AAGGGAGACT ACATCGGAGC AAAAGCCTTG ACAGTATCGA CTTCTGTGAA
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AGCACACAGT GACGCTTCAC
    AGGGCTCCTG GGTTTGGATT TGGAATTGCA ATCTCTGGTG GAAGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACCTCCAT AGTGATTTCT GATGTGTTAA AAGGAGGGCC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAACGTTGAA
    CATGCTTTTG CTGTTCAGCA GCTAAGGAAG AGTGGGAAAA ACGCAAAAAT TACTATCCGA
    AGGAAGAAGA AAGTTCAGAT CCCTGTAAGT CACCCAGATC CTGAGCCGGT GTCTGATAAT
    GAAGACGATA GTTATGACGA AGAAGTGCAT GACCCAAGAG CTGGCCGCGG TGCTTTAGCG
    AACAGAAGGA GCGAGAAGAG CTGGGCAAGG GATAGGAGTG CAAGCAGGGA GAGGAGCCTG
    TCCCCTCGCT CGGACAGGCG GTCCGTGGCC TCCAGTCAGC CCGCAAAGCC CACCAAGGTC
    ACACTGGTGA AGTCTCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT
    GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GTAACATTCA AGAAGGGGAT
    GTTGTCTTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAAACA
    CTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTGC AAAGAGATGA GCGGGCTACC
    TTACTGAACG TCCCTGACCT TTCGGATAGT ATCCATTCTG CTAATGCCTC GGAAAGAGAT
    GACATTTCAG AAATTCAGTC ACTAGCATCA GACCATTCAG GTCGCTCGCA TGACAGGCCA
    CCCCGCCGCA GCCAGTCACG ATCTCCTGAC CAACGTTCAG AGCCCTCCGA TCATTCCACG
    CAGTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAAGA GCGAATGTCT
    AAACCTGGGG CCATCTCAAC TCCTGTAAAA CATGTAGACG ATCATCCACC CAAAGCAGTG
    GAAGAAGTTA CAGTTGAGAA AAATGAGAAG CAGACGCCCA CTCTTCCAGA ACCGAAACCT
    GTGTATGCTC AAGTTGGACA ACCAGATGTG GATTTACCCG TCAGCCCTTC TGATGGTGCT
    CTGCCTAATT CAGCTCATGA AGACGGGATA CTTAGGCCCA GCATGAAACT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGACTA GCTGGTGGAA ATGATGTCGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAAGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTGA ACAATGTAGA TTTCACAAAT ATCATAAGGG AAGAGGCCGT CCTTTTCCTC
    CTTGACCTCC CTAAAGGTGA AGAAGTGACC ATACTGGCTC AGAAGAAGAA GGACGTTTAT
    CGCCGCATTG TAGAATCAGA TGTAGGAGAT TCATTCTATA TTAGAACGCA TTTTGAATAT
    GAAAAAGAAT CTCCTTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG GGTCGTGGAT
    ACTTTATACA ATGGAAAGCT GGGCTCTTGG CTTGCCATTC GAATTGGCAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AACAGTTAGC CAGTGTACAG
    TACACACTTC CAAAGACAGC GGGTGGTGAT CGGGCAGACT TCTGGAGGTT TCGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGACT TGTCAGCTCA GCCAGTTCAA
    ACTAAGTTCC CAGCTTATGA AAGGGTTGTT CTTCGAGAAG CTGGATTCCT AAGACCTGTA
    ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGT TGGCAAGAGA GGAGCCAGAT
    ATCTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGGA CTGACCATCG TAGCTCTGGC
    ATCATTCGCC TTCATACAAT AAAGCAAATC ATAGATCAGG ATAAACATGC TTTATTAGAT
    GTAACGCCAA ATGCGGTTGA TCGTCTTAAT TATGCGCAGT GGTATCCAAT TGTTGTGTTC
    CTTAACCCTG ACTCTAAGCA AGGTGTAAAA ACAATGAGGA TGAGGTTGTG TCCGGAGTCT
    CGGAAAAGCG CCAGGAAGCT ATATGAACGC TCTCATAAGC TTCGTAAGAA CAATCACCAT
    CTCTTCACAA CTACAATTAA CTTAAACTCA ATGAATGATG GTTGGTACGG TGCCCTGAAA
    GAAGCGATTC AGCAGCAACA GAACCAGCTG GTGTGGGTCT CTGAGGGGAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAC GATCGTCTGT CCTACCTGTC AGCCCCAGGT
    AGTGAGTACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTGGGG
    ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA TTCCTCTGGA
    ATGCATCATG AAAACCAGAC ATACCCTCCT TACTCACCAC AAGCGCAGCC ACAAGCTATT
    CATAGAATAG ACTCCCCTGG ACTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCCTCATCT
    CCAGTCCCTT ACCTTTCGCC TGAAACAACC CCAGCATCAT CAGCCTCTGC AGTTAATCAT
    AATGTCAGTG TAACTAATGT CAGCCTGGAG GAGCCTGCCC CAGCCCCTCC CACCTCGCAC
    GCATCACAGC CTGGTTGTTT AGGAGCACCA AGTGCTGAGG CAGCTCACGT AGTGCTCAGA
    GGTGAAGGAC CACCATTGCC GCCGCATGCA GACCCAGCAA AGGTGTACAG GAAGGAGCCG
    TATTCTGAAG AAATGATGAG ACAAAACCAT ATTTTAAAAC AACCAGCTCT TGGTCACCCA
    GGGCAGAGGC CAGATAAAGA GCCAAATCTA GCCTATGAAC CCCAACTTCC ATATATAGAA
    AAACAAGCCA GCAGAGACCT TGAGCAGCCG TCATACAGGT ATGAGGTCTC AAGCTACACA
    GACCAGTTTT CTCGGAACTA TGACCATCGC CTACGGTTTG AAGATCGAAT CCCTACCTAT
    GAAGACCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTTTGAGAAT
    CAGCATCCCC GAGACCTGGA CTCCAGACAA CATCCCGAAG AGGCTTCAGA ACGAGGTTAT
    TTCCAGCGTT TTGAAGAGCC AGCCCCTCTG TCGTACGACA GTAGAACACG CTATGAGCAG
    CTGCCTCGAA CCTCTACTCT ACGACATGAA GAGCAGCCAG CCCCTGCATA TGAGGTGCAC
    AACAGGTACA GGCCAGAGGC ACAGCCCTAT TCTTCAACAG GCCCTAAGTC ATCTGAGCCC
    AAGCAGTACT TTGACCAGTA CCCGCGAAGT TATGAGCAAG TTCCACCACC AGGCTTTACC
    TCCAAAACAG GCCATTACGA GCCTCTCCAT GGTGCTGCAG TTGTCCCTCC TCTGATACCT
    TCCTCTCAAC AAAAGCCAGA AGTCCTGCCC TCGGCTACCA AACCACAGCC TCCACCCCCA
    ACCCTAACTG AGGAGGAGGA GGATCCAGCA ATGAAACCAC AGTCTGTGCT CACCAGAGTC
    AAAATGTTTG AAAACAAAAG ATCTGCGTCT TTGGAGAACA AGAAAGATGT GAATGACACT
    GCCAGCTTTA AGCCTCCGGA AGTAGCATCT AAACCTCCAG GTGCTTCTCT TGCTGGCCCT
    AAACCTGTCC CTCAGAGTCA GTTTAGTGAG CACGACAAAA CGCTCTACAG GCTCCCAGAG
    CCTCAGAAAC CTCAAGTGAA GCCACCCGAA GATATTGTTC GATCAAATCA TTACGACCCT
    GAAGAGGATG AAGAATATTA CCGGAAACAG CTCTCCTACT TTGACCGAAG AAGTTTTGAG
    AGCAAGCCTT CTGCACATCT TCCTGCTGGC CATCACTCAG AGCCTGCTAA GCCAGTCCAT
    TCTCAGAGCC AGCCCAACTT CTCTAGTTAC TCTTCAAAGG GAAAACCCGA AACTGATGCT
    GTGGATAGAT CATTCAGTGA GAAACGTTAT GATCCAGCCC AGGCCACGCC TCCTCCTCCT
    CCGTTGCCCT CACAGTACAG CCAGCCAGCT CCACCTCTGT CCAGCTCTTC TCTCCACATA
    CATTCCAAGG GCGCCCAGGG TGAAGGCAAC TCAGTATCAT TGGATTTTCA GAACTCATAT
    ATGTCCAAAC CAGACCCACC CCCATCTCAG AGTAAACCAG CAACTTTCAG ACCACCAACT
    CGAGAAGACC CCCCTCAGAC TTTCTATCCG CAGAAAAGTT TCCCAGACAA AGCTCCAGTT
    AACGGAGCTG AGCAGACTCA GAAAACCATC ACTCCGGTGT ACAATCGATT CACACCAAAG
    CCGTACACAA GTTCTGCCCG GCCATTTGAA CGCAAATTTG AAAGTCCGAA GTTCAACCAT
    AATCTTCTGC CAAGTGAAAC TGTACATAAA CCTGAATTGT CTTCAAAAAC TCCCACTTCC
    CCAAAAACTC TTATGAAAGC TCATAGTTCA ACACAGCCTC CAGAGTTTGA CAGTGGAGTT
    GAAACTTTCT CTGTTCACAC AGATAAGCCT AAATATCAAA TGAATAATAT CAGCACCATG
    CCTAAAGCTG TCCCTGTGAG TCCTTCAGCT GTGGAAGAAG ATGAAGATGA GGATGGTCAT
    ACTGTAGTGG CTACAGCTCG TGGCATTTTT AACAGCAATG GTGGTGTGTT GAGTTCCATA
    GAAACCGGTG TTAGTATAAT TATCCCACAA GGAGCCATTC CTGAAGGAAT TGAGCAAGAA
    ATCTATTTCA AAGTCTGCAG AGACAATAGC ATTCTCCCAC CTTTAGATAA AGAGAAAGGT
    GAAACTCTGC TGAGCCCCCT AGTGATGTGT GGGCCCCATG GCCTCAAGTT CCTGAAGCCC
    GTGGAGCTAC GCTTGCCACA CTGTGCGTCC ATGACTCCTG ACGGTGACCC TAAAACCTGG
    CAAAACAAGT GTCTTCCTGG AGATCCGAAT TACCTTGTTG GAGCCAACTG TGTTTCTGTC
    CTGATTGACC ACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.