TJP1 cDNA ORF clone, Gallus gallus(chicken)

The following TJP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TJP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa143492 XM_015278975.2
Latest version!
Gallus gallus tight junction protein 1 (TJP1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OGa143493 XM_015278976.2
Latest version!
Gallus gallus tight junction protein 1 (TJP1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OGa143494 XM_015278977.2
Latest version!
Gallus gallus tight junction protein 1 (TJP1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OGa143495 XM_015278979.2
Latest version!
Gallus gallus tight junction protein 1 (TJP1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OGa143496 XM_015278981.2
Latest version!
Gallus gallus tight junction protein 1 (TJP1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OGa40350 XM_015278975.1
Latest version!
Gallus gallus tight junction protein 1 (TJP1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa40351 XM_015278976.1
Latest version!
Gallus gallus tight junction protein 1 (TJP1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa40352 XM_015278977.1
Latest version!
Gallus gallus tight junction protein 1 (TJP1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa40354 XM_015278979.1
Latest version!
Gallus gallus tight junction protein 1 (TJP1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa40355 XM_015278980.2
Latest version!
Gallus gallus tight junction protein 1 (TJP1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa40356 XM_015278981.1
Latest version!
Gallus gallus tight junction protein 1 (TJP1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa40353 XM_015278978.1
Latest version!
Gallus gallus tight junction protein 1 (TJP1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
View Old Accession Versions >>
OGa40355 XM_015278980.1 Gallus gallus tight junction protein 1 (TJP1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa143492
    Clone ID Related Accession (Same CDS sequence) XM_015278975.2
    Accession Version XM_015278975.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5589bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product tight junction protein ZO-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006097.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015278975.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGCAGCGGC TGGAGAGACT CTGTCGGTGG CGGCGGCGGG GACGCGGCAG GGACAGGGGG 
    GGCGACGGCA AAATGAAGTA TCAGAAATAC CTGACTGTCT TGCAGATGGC CATAGGGGTG
    ACCGCCTCCA ACAGGGGCAG TCTGATGCCC TTAAAGAGGA AGCTGTGGGT AACTCCATCT
    TCCGAAAATC CCAATGGTGC TACCTGTAGT GTAAGTCAGG GAAAGCCTTC TTTAAGAAGA
    ATAAAAGGGA GAATACACAG AAGCAAAAGC CTTGATAGCA TTGATTTCTG TGAATTCACT
    AGTGCAACAA TGGAAGAAAC AGCTATATGG GAACAACACA CGGTGACTCT TCACAGGGCT
    CCTGGATTTG GGTTTGGTAT TGCAATATCT GGAGGAAGGG ATAATCCTCA CTTTCAAAGT
    GGTGAGACCT CAATAGTTAT TTCTGATGTG CTGAAAGGAG GCCCAGCAGA AGGACTGCTA
    CAAGAAAATG ACCGTGTTGC AATGGTTAAT GGAGTTTCAA TGGATAATGT TGAGCACGCA
    TTTGCTGTTC AGCAGCTGAG GAAAAGTGGG AAGAATGCCA AAATTACTAT CCGAAGGATG
    AAGAAAATTC AGATTCCAGT AGCTCGACAG GAACCTGAGC CAGTGTCTGA AAATGAGGAC
    GATAGCTATG ATGAAGAGAT ACGGGATCCA AGGACCAGCC GTGGTGCTTC CAGTGCCAAC
    AGAAGGCATG AGAAGAGCTG GGTACGAGAC CGAAGTGCAA GCAGAGAAAG AAGCTTATCA
    CCAAGATCTG ACAGAAGATC GGTTACTTCC AGTCAGCCTG CAAAACCTAC CAAAGTAACG
    TTGGTGAAAT CAAGGAAAAA TGAAGAGTAT GGTCTACGGT TGGCAAGCCA CATATTTGTG
    AAAGAAATAT CACAGGATAG CCTTGCAGCA AGAGATGGCA ATATTCAGGA AGGGGATGTT
    GTTCTGAAGA TAAATGGCAC AGTGACAGAA AATATGTCCT TAGCAGATGC AAAAACGCTA
    ATAGAAAGGT CCAAAGGCAA GTTGAAGATG GTGGTTCAGC GAGATGAACG AGCTACACTG
    TTGAATGTCC CTGATCTTTC TGACAGTATT CATTCTGCTA ATGCTTCGGA AAGAGATGAC
    ATCTCAGAAA TTCAGTCGCT GGCATCAGAT CATTCCAACC GATCACATGA CAGGCCCCGC
    CGCAGTCGTT CACGATCTCC TGACCAGAGG TCAGAGCCTT CAGACCATTC CAGACATTCT
    CCACAGCAGC CCAGCAACGG CAGTCTTCGA AGCAGAGAAG ATGAAAGGGT AACTAAACCA
    GGAGCCATCT CTACACCTGT AAAGAATGCA GATGATATTT CTAAAACTAT GGAAGAGGTG
    TCAGTCGAAA GAACTGAAAA ACAAACACCA CCGCTCCCAG AACCAAAGCC AGTATATGCA
    CAAGGAGGTC AGCCAGATGT GGATTTACCA GTCAGTCCGT CTGATGGTCC TTTACCCAAT
    TCAACCCATG AAGATGGAAT GCTTCGGCCA AGCATGAAAC TGGTAAAATT CAGAAAAGGA
    GATAGTGTGG GCCTGCGGCT CGCTGGTGGC AATGATGTTG GCATATTTGT AGCTGGTGTT
    CTAGAGGACA GCCCTGCAGC GAAAGAAGGA TTAGAGGAAG GAGACCAAAT TCTCAGGGTT
    AATAACGTAG ACTTCACAAA TATCATCAGA GAAGAAGCTG TCCTTTTTTT GCTTGATCTC
    CCTAAAGGCG AAGAAGTAAC CATTTTGGCA CAGAAGAAGA AAGATGTTTA TCGTCGCATT
    GTTGAGTCTG ATGTAGGGGA TTCTTTCTAT ATCAGAACTC ATTTTGAATA TGAAAAGGAA
    TCTCCTTATG GACTAAGCTT CAACAAAGGT GAAGTGTTTC GGGTTGTGGA CACGCTATAT
    AATGGCAAAC TGGGTTCGTG GCTGGCAATT CGAATTGGCA AGAATCATAA GGAAGTTGAA
    AGAGGTATCA TACCTAACAA AAACAGAGCT GAACAACTAG CTAGTGTACA GTACACGCTT
    CCAAAGACAG CAGGAGGAGA TCGAGCGGAT TTCTGGAGAT TCCGAGGTTT GCGTAGCTCA
    AAAAGAAATC TCCGAAAAAG TAGAGAAGAT CTTTCTGCTC AGCCTGTTCA GACAAAGTTC
    CCTGCCTATG AAAGAGTTGT TCTTCGGGAA GCTGGGTTTC TCAGACCTGT GACCATTTTT
    GGTCCAATAG CTGATGTTGC ACGAGAGAAA CTAGCCAGAG AAGAACCAGA TATCTTTCAA
    ATTGCAAAAA GTGAACCGAG AGATGCTGGT ACTGACCAAC GTAGTTCTGG CATTATTCGT
    CTTCATACGA TAAAACAGAT AATTGATAGA GACAAACACG CTTTATTAGA TGTCACTCCT
    AATGCAGTGG ACCGTCTGAA TTATGCACAG TGGTATCCTA TTGTAGTGTT TCTGAACCCT
    GATTCTAAGC AGGGTGTGAA GACCATGAGA ATGAGGCTGT GCCCAGAATC ACGAAAAAGT
    GCCAGGAAGC TATATGAAAG AGCTCACAAG CTACGCAAAA ATAATCACCA CCTCTTTACA
    ACTACCATTA ACTTGAATTC AATGAATGAA GGATGGTATG GAGCTCTAAA AGAAGCAATT
    CAGCAACAAC AAAACCAACT GGTGTGGGTT TCTGAAGGAA AGGCAGATGG TGCTACAAGT
    GATGACCTTG ATTTACATGA TGACCGCCTG TCTTACCTCT CAGCTCCTGG TAGTGAATAT
    TCTATGTACA GTACAGACAG CAGACATACA TCTGACTATG AAGACACAGA TACAGAAGGT
    GGTGCTTATA CTGATCAAGA ACTGGATGAA ACTCTGAACG ATGAGGTTGG AACTCCTCCT
    GAATCTGCTA TTACACGTTC TTCTGAACCC GTTAGGGAGG ATTCTTCTGG AATGCATCAT
    GAAACTCAAA CTTACACTAG TTACGCATCT CAAGCTCAGC CTCAGCCAAA TCTTAGAATA
    GATTCTTCAG GATTTAAAAC AACTGCTTCT CAGCCGAAAG CAGAAGCATC ACCTGCAGTC
    CCTTACCTTT CCCCGTCGCC TGAATCAAAC CCAGCAACCT CATCAACCTC TGCAGTAAAT
    GCTAATGTAA ACCTAACTAA TGTCAGACTA GAGGAGCCTA CTGCTGCTCC TTACAACTCT
    TATCAACCAC AAGCGGGCCC CTTAAGAACA TCAAGTACTG AGGGAGCTCA TGTAGTGCTA
    AGAGATCAAG AGCCATCATC CTTACCGCCG CATATAGATC CAGCAAAGGT ATACAGAAAG
    GATCCATATA TTAGTGAAGA AGCATCCAGA CAAAGCTATG TCTTAAAACA GCCAGCTGTT
    AATCACCCAG TACAAAGACA GGAAAGAGAC CCAAATCTGA TCTATGAATC TCAGACTCAG
    TATGCAGAAA AACAACCAAG TAGGGAGTAC GAGCAGTCAA CATACAGGTA TGATTCTACA
    AACTATGTAG ATCAATTTTC TCGTGGGTAT GACCCTCGTC TACATTATGA AGATCGTGCG
    CCTCCCTATG AGGAGCACTG GTCATATTAT GATGAAAAAC AGCCCTACAA TCAACCAAGA
    ACAGCTTATG AAAGCCAGCC TCCTAGAGAT CTTGATTCCA GACAAAATAC AGAAGAGAGC
    ACAGAACGCA GCTATTATCC GGCACAACCC CGATTTGAGG AACCTCCTCC GATGAGCTAC
    GATGGCAGAC CTCGCTATGA GCATGCACCC AAAAACTTCA GCTTGCCACA AGTGCGGTAT
    GAAGATCAAC ATCCTGTTGG CTATGACACA CATGCGCATG GTAGATATAA ACAAGAAGCT
    CAGCCATACC AGTCAGCCAT ATCTCGATCT CCTGAACCCA AGCAGTACTT TGATCCACAT
    GTGAGGGGCT ATGAGCAAGG TCCTCCTCAA GCTTACAATG CAAAAGCTGG ACAGTTTGAG
    CCTTCTCATA GCACTTCAGG TGTTTCTCTT CCTCCTCCCG CTTCTTCACA AACAAAACCA
    GAAGTTTTGC CTTCCAACAG TAAACCACTG CCTACACCAC CATCTCTAGC AGAAGAAGAG
    GAGGATCCAG CCATGAAACC ACAGTCTGTG CGAAGTAGAG TTAAGATATT TGAACGAAGA
    AGATCGCCAT CTCTGGAAAA AATGAAGGAC CCCAGTGACA CGTCAGTTGT CAAACCTCCA
    GAGCTAGCAC CGAAGCCTAC GCTCCCAGCC ATGAGTGGTC CAAAACCAAC TTCTCAAAGC
    CAGTATGAAC ATGATAAAAC AACTTACAGG GCCCCAGAAC CACAGAGACC TCAAGTGAAG
    CCACCTGAAG ATATCGTGCG TTCAAATCAT TATGATCCCG AAGAAGATGA AGAGTATTAC
    AGAAAGCAGC TCTCATACTT TGATCGCAGG AATTTTGAAA ATAAGCCTTC TGCACAGGTT
    CCTGCCAGCC ATCATTCTGA CTCCACTAAA CCAATACATT CACAAAATCA GCTGAATTTC
    TCCAATTATT CTAAGGGGAA GCCAACTGAT GCTGAACCAA TGGATAGATC TGTTGGTGAC
    AAGCGCTATG AGCCAATCCC TCAAGTTACA ACTCCTCCTC CTGCTCCTTC AGTACAGTAT
    ACACAACCTC AGTCAATTAA TAGTCCTGTC CTGTCTCTCC CAGCGCATCA CAAGCCTGCA
    CTTTCTGAAG TTAACTCTGT GTCTGACCCT CCTCCACCTC AGAATAAGCC AGCAATTTTC
    AGATCCTCTA GAGAGGACAC TGTGCAGTCT ACTTTTTATC CTCAGAAAAG CTTCCCTGAC
    AAAGGTCCTG TTAATGGAAC TGAACAGGTT CAGAAAACAG TCACTCCTTC TTACAACCGT
    TTTACAACAA AACCTTACAC TAGTGCTGCG AGGCCCTTTG AGCGCAAGTT TGAAAGTCCT
    AAATTCAACC ATAATCTCTT GCCAAATGAA GCTCAACATA AACCAGAGTT GCCATCAAAA
    TCTCCAAATT CTCCTCAACC AATTTTGAAA GCACACAGCT CATCACAGCC TCCTGAGTTT
    GACAGTGGAA TGGACACCTT CACTGTACAG GTTGACAAGC CTAAATATCA ACCAAATAAT
    GTAAATGCTG TGCCTAAAGC CATTCCTGTA AGCCCTTCAG CACTAGAGGA TGAGGAGGAA
    GAAGACGGAC ACACTGTTGT AGCCACAGCA AGAGGTGTAT TCAACAGCAA TGGTGGCGTA
    TTGAGCTCCA TAGAGACTGG AGTTAGTATT ATTATACCAC AAGGAGCCAT TCCAGAGGGA
    ATAGAGCAAG AAATATATTT TAAAGTCTGC AGAGACAACA GTATACTTCC ACCTTTGGAC
    AAAGAGAAAG GTGAAACACT TCTTAGCCCC TTGGTAATGT GTGGGCCTCA CGGACTAAAA
    TTCCTGAAGC CAGTGGAGCT GCGCCTGCCA CACTGTGCGT CTATGACCCC TGATGGTTGG
    TCTTTTGCTC TAAAATCCTC TGACTCCTCG TCGGGTGACC CCAAAACCTG GCAGAACAAG
    TCTCTTCCTG GAGATCCAAA CTATCTTGTT GGAGCAAACT GTGTCTCAGT CCTAATTGAC
    CACTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015134461.2
    CDS16..5604
    Translation

    Target ORF information:

    RefSeq Version XM_015278975.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus tight junction protein 1 (TJP1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015278975.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGCAGCGGC TGGAGAGACT CTGTCGGTGG CGGCGGCGGG GACGCGGCAG GGACAGGGGG 
    GGCGACGGCA AAATGAAGTA TCAGAAATAC CTGACTGTCT TGCAGATGGC CATAGGGGTG
    ACCGCCTCCA ACAGGGGCAG TCTGATGCCC TTAAAGAGGA AGCTGTGGGT AACTCCATCT
    TCCGAAAATC CCAATGGTGC TACCTGTAGT GTAAGTCAGG GAAAGCCTTC TTTAAGAAGA
    ATAAAAGGGA GAATACACAG AAGCAAAAGC CTTGATAGCA TTGATTTCTG TGAATTCACT
    AGTGCAACAA TGGAAGAAAC AGCTATATGG GAACAACACA CGGTGACTCT TCACAGGGCT
    CCTGGATTTG GGTTTGGTAT TGCAATATCT GGAGGAAGGG ATAATCCTCA CTTTCAAAGT
    GGTGAGACCT CAATAGTTAT TTCTGATGTG CTGAAAGGAG GCCCAGCAGA AGGACTGCTA
    CAAGAAAATG ACCGTGTTGC AATGGTTAAT GGAGTTTCAA TGGATAATGT TGAGCACGCA
    TTTGCTGTTC AGCAGCTGAG GAAAAGTGGG AAGAATGCCA AAATTACTAT CCGAAGGATG
    AAGAAAATTC AGATTCCAGT AGCTCGACAG GAACCTGAGC CAGTGTCTGA AAATGAGGAC
    GATAGCTATG ATGAAGAGAT ACGGGATCCA AGGACCAGCC GTGGTGCTTC CAGTGCCAAC
    AGAAGGCATG AGAAGAGCTG GGTACGAGAC CGAAGTGCAA GCAGAGAAAG AAGCTTATCA
    CCAAGATCTG ACAGAAGATC GGTTACTTCC AGTCAGCCTG CAAAACCTAC CAAAGTAACG
    TTGGTGAAAT CAAGGAAAAA TGAAGAGTAT GGTCTACGGT TGGCAAGCCA CATATTTGTG
    AAAGAAATAT CACAGGATAG CCTTGCAGCA AGAGATGGCA ATATTCAGGA AGGGGATGTT
    GTTCTGAAGA TAAATGGCAC AGTGACAGAA AATATGTCCT TAGCAGATGC AAAAACGCTA
    ATAGAAAGGT CCAAAGGCAA GTTGAAGATG GTGGTTCAGC GAGATGAACG AGCTACACTG
    TTGAATGTCC CTGATCTTTC TGACAGTATT CATTCTGCTA ATGCTTCGGA AAGAGATGAC
    ATCTCAGAAA TTCAGTCGCT GGCATCAGAT CATTCCAACC GATCACATGA CAGGCCCCGC
    CGCAGTCGTT CACGATCTCC TGACCAGAGG TCAGAGCCTT CAGACCATTC CAGACATTCT
    CCACAGCAGC CCAGCAACGG CAGTCTTCGA AGCAGAGAAG ATGAAAGGGT AACTAAACCA
    GGAGCCATCT CTACACCTGT AAAGAATGCA GATGATATTT CTAAAACTAT GGAAGAGGTG
    TCAGTCGAAA GAACTGAAAA ACAAACACCA CCGCTCCCAG AACCAAAGCC AGTATATGCA
    CAAGGAGGTC AGCCAGATGT GGATTTACCA GTCAGTCCGT CTGATGGTCC TTTACCCAAT
    TCAACCCATG AAGATGGAAT GCTTCGGCCA AGCATGAAAC TGGTAAAATT CAGAAAAGGA
    GATAGTGTGG GCCTGCGGCT CGCTGGTGGC AATGATGTTG GCATATTTGT AGCTGGTGTT
    CTAGAGGACA GCCCTGCAGC GAAAGAAGGA TTAGAGGAAG GAGACCAAAT TCTCAGGGTT
    AATAACGTAG ACTTCACAAA TATCATCAGA GAAGAAGCTG TCCTTTTTTT GCTTGATCTC
    CCTAAAGGCG AAGAAGTAAC CATTTTGGCA CAGAAGAAGA AAGATGTTTA TCGTCGCATT
    GTTGAGTCTG ATGTAGGGGA TTCTTTCTAT ATCAGAACTC ATTTTGAATA TGAAAAGGAA
    TCTCCTTATG GACTAAGCTT CAACAAAGGT GAAGTGTTTC GGGTTGTGGA CACGCTATAT
    AATGGCAAAC TGGGTTCGTG GCTGGCAATT CGAATTGGCA AGAATCATAA GGAAGTTGAA
    AGAGGTATCA TACCTAACAA AAACAGAGCT GAACAACTAG CTAGTGTACA GTACACGCTT
    CCAAAGACAG CAGGAGGAGA TCGAGCGGAT TTCTGGAGAT TCCGAGGTTT GCGTAGCTCA
    AAAAGAAATC TCCGAAAAAG TAGAGAAGAT CTTTCTGCTC AGCCTGTTCA GACAAAGTTC
    CCTGCCTATG AAAGAGTTGT TCTTCGGGAA GCTGGGTTTC TCAGACCTGT GACCATTTTT
    GGTCCAATAG CTGATGTTGC ACGAGAGAAA CTAGCCAGAG AAGAACCAGA TATCTTTCAA
    ATTGCAAAAA GTGAACCGAG AGATGCTGGT ACTGACCAAC GTAGTTCTGG CATTATTCGT
    CTTCATACGA TAAAACAGAT AATTGATAGA GACAAACACG CTTTATTAGA TGTCACTCCT
    AATGCAGTGG ACCGTCTGAA TTATGCACAG TGGTATCCTA TTGTAGTGTT TCTGAACCCT
    GATTCTAAGC AGGGTGTGAA GACCATGAGA ATGAGGCTGT GCCCAGAATC ACGAAAAAGT
    GCCAGGAAGC TATATGAAAG AGCTCACAAG CTACGCAAAA ATAATCACCA CCTCTTTACA
    ACTACCATTA ACTTGAATTC AATGAATGAA GGATGGTATG GAGCTCTAAA AGAAGCAATT
    CAGCAACAAC AAAACCAACT GGTGTGGGTT TCTGAAGGAA AGGCAGATGG TGCTACAAGT
    GATGACCTTG ATTTACATGA TGACCGCCTG TCTTACCTCT CAGCTCCTGG TAGTGAATAT
    TCTATGTACA GTACAGACAG CAGACATACA TCTGACTATG AAGACACAGA TACAGAAGGT
    GGTGCTTATA CTGATCAAGA ACTGGATGAA ACTCTGAACG ATGAGGTTGG AACTCCTCCT
    GAATCTGCTA TTACACGTTC TTCTGAACCC GTTAGGGAGG ATTCTTCTGG AATGCATCAT
    GAAACTCAAA CTTACACTAG TTACGCATCT CAAGCTCAGC CTCAGCCAAA TCTTAGAATA
    GATTCTTCAG GATTTAAAAC AACTGCTTCT CAGCCGAAAG CAGAAGCATC ACCTGCAGTC
    CCTTACCTTT CCCCGTCGCC TGAATCAAAC CCAGCAACCT CATCAACCTC TGCAGTAAAT
    GCTAATGTAA ACCTAACTAA TGTCAGACTA GAGGAGCCTA CTGCTGCTCC TTACAACTCT
    TATCAACCAC AAGCGGGCCC CTTAAGAACA TCAAGTACTG AGGGAGCTCA TGTAGTGCTA
    AGAGATCAAG AGCCATCATC CTTACCGCCG CATATAGATC CAGCAAAGGT ATACAGAAAG
    GATCCATATA TTAGTGAAGA AGCATCCAGA CAAAGCTATG TCTTAAAACA GCCAGCTGTT
    AATCACCCAG TACAAAGACA GGAAAGAGAC CCAAATCTGA TCTATGAATC TCAGACTCAG
    TATGCAGAAA AACAACCAAG TAGGGAGTAC GAGCAGTCAA CATACAGGTA TGATTCTACA
    AACTATGTAG ATCAATTTTC TCGTGGGTAT GACCCTCGTC TACATTATGA AGATCGTGCG
    CCTCCCTATG AGGAGCACTG GTCATATTAT GATGAAAAAC AGCCCTACAA TCAACCAAGA
    ACAGCTTATG AAAGCCAGCC TCCTAGAGAT CTTGATTCCA GACAAAATAC AGAAGAGAGC
    ACAGAACGCA GCTATTATCC GGCACAACCC CGATTTGAGG AACCTCCTCC GATGAGCTAC
    GATGGCAGAC CTCGCTATGA GCATGCACCC AAAAACTTCA GCTTGCCACA AGTGCGGTAT
    GAAGATCAAC ATCCTGTTGG CTATGACACA CATGCGCATG GTAGATATAA ACAAGAAGCT
    CAGCCATACC AGTCAGCCAT ATCTCGATCT CCTGAACCCA AGCAGTACTT TGATCCACAT
    GTGAGGGGCT ATGAGCAAGG TCCTCCTCAA GCTTACAATG CAAAAGCTGG ACAGTTTGAG
    CCTTCTCATA GCACTTCAGG TGTTTCTCTT CCTCCTCCCG CTTCTTCACA AACAAAACCA
    GAAGTTTTGC CTTCCAACAG TAAACCACTG CCTACACCAC CATCTCTAGC AGAAGAAGAG
    GAGGATCCAG CCATGAAACC ACAGTCTGTG CGAAGTAGAG TTAAGATATT TGAACGAAGA
    AGATCGCCAT CTCTGGAAAA AATGAAGGAC CCCAGTGACA CGTCAGTTGT CAAACCTCCA
    GAGCTAGCAC CGAAGCCTAC GCTCCCAGCC ATGAGTGGTC CAAAACCAAC TTCTCAAAGC
    CAGTATGAAC ATGATAAAAC AACTTACAGG GCCCCAGAAC CACAGAGACC TCAAGTGAAG
    CCACCTGAAG ATATCGTGCG TTCAAATCAT TATGATCCCG AAGAAGATGA AGAGTATTAC
    AGAAAGCAGC TCTCATACTT TGATCGCAGG AATTTTGAAA ATAAGCCTTC TGCACAGGTT
    CCTGCCAGCC ATCATTCTGA CTCCACTAAA CCAATACATT CACAAAATCA GCTGAATTTC
    TCCAATTATT CTAAGGGGAA GCCAACTGAT GCTGAACCAA TGGATAGATC TGTTGGTGAC
    AAGCGCTATG AGCCAATCCC TCAAGTTACA ACTCCTCCTC CTGCTCCTTC AGTACAGTAT
    ACACAACCTC AGTCAATTAA TAGTCCTGTC CTGTCTCTCC CAGCGCATCA CAAGCCTGCA
    CTTTCTGAAG TTAACTCTGT GTCTGACCCT CCTCCACCTC AGAATAAGCC AGCAATTTTC
    AGATCCTCTA GAGAGGACAC TGTGCAGTCT ACTTTTTATC CTCAGAAAAG CTTCCCTGAC
    AAAGGTCCTG TTAATGGAAC TGAACAGGTT CAGAAAACAG TCACTCCTTC TTACAACCGT
    TTTACAACAA AACCTTACAC TAGTGCTGCG AGGCCCTTTG AGCGCAAGTT TGAAAGTCCT
    AAATTCAACC ATAATCTCTT GCCAAATGAA GCTCAACATA AACCAGAGTT GCCATCAAAA
    TCTCCAAATT CTCCTCAACC AATTTTGAAA GCACACAGCT CATCACAGCC TCCTGAGTTT
    GACAGTGGAA TGGACACCTT CACTGTACAG GTTGACAAGC CTAAATATCA ACCAAATAAT
    GTAAATGCTG TGCCTAAAGC CATTCCTGTA AGCCCTTCAG CACTAGAGGA TGAGGAGGAA
    GAAGACGGAC ACACTGTTGT AGCCACAGCA AGAGGTGTAT TCAACAGCAA TGGTGGCGTA
    TTGAGCTCCA TAGAGACTGG AGTTAGTATT ATTATACCAC AAGGAGCCAT TCCAGAGGGA
    ATAGAGCAAG AAATATATTT TAAAGTCTGC AGAGACAACA GTATACTTCC ACCTTTGGAC
    AAAGAGAAAG GTGAAACACT TCTTAGCCCC TTGGTAATGT GTGGGCCTCA CGGACTAAAA
    TTCCTGAAGC CAGTGGAGCT GCGCCTGCCA CACTGTGCGT CTATGACCCC TGATGGTTGG
    TCTTTTGCTC TAAAATCCTC TGACTCCTCG TCGGGTGACC CCAAAACCTG GCAGAACAAG
    TCTCTTCCTG GAGATCCAAA CTATCTTGTT GGAGCAAACT GTGTCTCAGT CCTAATTGAC
    CACTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa143493
    Clone ID Related Accession (Same CDS sequence) XM_015278976.2
    Accession Version XM_015278976.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5550bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product tight junction protein ZO-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006097.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015278976.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCAGCGGC TGGAGAGACT CTGTCGGTGG CGGCGGCGGG GACGCGGCAG GGACAGGGGG 
    GGCGACGGCA AAATGAAGTA TCAGAAATAC CTGACTGTCT TGCAGATGGC CATAGGGGTG
    ACCGCCTCCA ACAGGGGCAG TCTGATGCCC TTAAAGAGGA AGCTGTGGGT AACTCCATCT
    TCCGAAAATC CCAATGGTGC TACCTGTAGT GTAAGTCAGG GAAAGCCTTC TTTAAGAAGA
    ATAAAAGGGA GAATACACAG AAGCAAAAGC CTTGATAGCA TTGATTTCTG TGAATTCACT
    AGTGCAACAA TGGAAGAAAC AGCTATATGG GAACAACACA CGGTGACTCT TCACAGGGCT
    CCTGGATTTG GGTTTGGTAT TGCAATATCT GGAGGAAGGG ATAATCCTCA CTTTCAAAGT
    GGTGAGACCT CAATAGTTAT TTCTGATGTG CTGAAAGGAG GCCCAGCAGA AGGACTGCTA
    CAAGAAAATG ACCGTGTTGC AATGGTTAAT GGAGTTTCAA TGGATAATGT TGAGCACGCA
    TTTGCTGTTC AGCAGCTGAG GAAAAGTGGG AAGAATGCCA AAATTACTAT CCGAAGGATG
    AAGAAAATTC AGATTCCAGT AGCTCGACAG GAACCTGAGC CAGTGTCTGA AAATGAGGAC
    GATAGCTATG ATGAAGAGAT ACGGGATCCA AGGACCAGCC GTGGTGCTTC CAGTGCCAAC
    AGAAGGCATG AGAAGAGCTG GGTACGAGAC CGAAGTGCAA GCAGAGAAAG AAGCTTATCA
    CCAAGATCTG ACAGAAGATC GGTTACTTCC AGTCAGCCTG CAAAACCTAC CAAAGTAACG
    TTGGTGAAAT CAAGGAAAAA TGAAGAGTAT GGTCTACGGT TGGCAAGCCA CATATTTGTG
    AAAGAAATAT CACAGGATAG CCTTGCAGCA AGAGATGGCA ATATTCAGGA AGGGGATGTT
    GTTCTGAAGA TAAATGGCAC AGTGACAGAA AATATGTCCT TAGCAGATGC AAAAACGCTA
    ATAGAAAGGT CCAAAGGCAA GTTGAAGATG GTGGTTCAGC GAGATGAACG AGCTACACTG
    TTGAATGTCC CTGATCTTTC TGACAGTATT CATTCTGCTA ATGCTTCGGA AAGAGATGAC
    ATCTCAGAAA TTCAGTCGCT GGCATCAGAT CATTCCAACC GATCACATGA CAGGCCCCGC
    CGCAGTCGTT CACGATCTCC TGACCAGAGG TCAGAGCCTT CAGACCATTC CAGACATTCT
    CCACAGCAGC CCAGCAACGG CAGTCTTCGA AGCAGAGAAG ATGAAAGGGT AACTAAACCA
    GGAGCCATCT CTACACCTGT AAAGAATGCA GATGATATTT CTAAAACTAT GGAAGAGGTG
    TCAGTCGAAA GAACTGAAAA ACAAACACCA CCGCTCCCAG AACCAAAGCC AGTATATGCA
    CAAGGAGGTC AGCCAGATGT GGATTTACCA GTCAGTCCGT CTGATGGTCC TTTACCCAAT
    TCAACCCATG AAGATGGAAT GCTTCGGCCA AGCATGAAAC TGGTAAAATT CAGAAAAGGA
    GATAGTGTGG GCCTGCGGCT CGCTGGTGGC AATGATGTTG GCATATTTGT AGCTGGTGTT
    CTAGAGGACA GCCCTGCAGC GAAAGAAGGA TTAGAGGAAG GAGACCAAAT TCTCAGGGTT
    AATAACGTAG ACTTCACAAA TATCATCAGA GAAGAAGCTG TCCTTTTTTT GCTTGATCTC
    CCTAAAGGCG AAGAAGTAAC CATTTTGGCA CAGAAGAAGA AAGATGTTTA TCGTCGCATT
    GTTGAGTCTG ATGTAGGGGA TTCTTTCTAT ATCAGAACTC ATTTTGAATA TGAAAAGGAA
    TCTCCTTATG GACTAAGCTT CAACAAAGGT GAAGTGTTTC GGGTTGTGGA CACGCTATAT
    AATGGCAAAC TGGGTTCGTG GCTGGCAATT CGAATTGGCA AGAATCATAA GGAAGTTGAA
    AGAGGTATCA TACCTAACAA AAACAGAGCT GAACAACTAG CTAGTGTACA GTACACGCTT
    CCAAAGACAG CAGGAGGAGA TCGAGCGGAT TTCTGGAGAT TCCGAGGTTT GCGTAGCTCA
    AAAAGAAATC TCCGAAAAAG TAGAGAAGAT CTTTCTGCTC AGCCTGTTCA GACAAAGTTC
    CCTGCCTATG AAAGAGTTGT TCTTCGGGAA GCTGGGTTTC TCAGACCTGT GACCATTTTT
    GGTCCAATAG CTGATGTTGC ACGAGAGAAA CTAGCCAGAG AAGAACCAGA TATCTTTCAA
    ATTGCAAAAA GTGAACCGAG AGATGCTGGT ACTGACCAAC GTAGTTCTGG CATTATTCGT
    CTTCATACGA TAAAACAGAT AATTGATAGA GACAAACACG CTTTATTAGA TGTCACTCCT
    AATGCAGTGG ACCGTCTGAA TTATGCACAG TGGTATCCTA TTGTAGTGTT TCTGAACCCT
    GATTCTAAGC AGGGTGTGAA GACCATGAGA ATGAGGCTGT GCCCAGAATC ACGAAAAAGT
    GCCAGGAAGC TATATGAAAG AGCTCACAAG CTACGCAAAA ATAATCACCA CCTCTTTACA
    ACTACCATTA ACTTGAATTC AATGAATGAA GGATGGTATG GAGCTCTAAA AGAAGCAATT
    CAGCAACAAC AAAACCAACT GGTGTGGGTT TCTGAAGGAA AGGCAGATGG TGCTACAAGT
    GATGACCTTG ATTTACATGA TGACCGCCTG TCTTACCTCT CAGCTCCTGG TAGTGAATAT
    TCTATGTACA GTACAGACAG CAGACATACA TCTGACTATG AAGACACAGA TACAGAAGGT
    GGTGCTTATA CTGATCAAGA ACTGGATGAA ACTCTGAACG ATGAGGTTGG AACTCCTCCT
    GAATCTGCTA TTACACGTTC TTCTGAACCC GTTAGGGAGG ATTCTTCTGG AATGCATCAT
    GAAACTCAAA CTTACACTAG TTACGCATCT CAAGCTCAGC CTCAGCCAAA TCTTAGAATA
    GATTCTTCAG GATTTAAAAC AACTGCTTCT CAGCCGAAAG CAGAAGCATC ACCTGCAGTC
    CCTTACCTTT CCCCGTCGCC TGAATCAAAC CCAGCAACCT CATCAACCTC TGCAGTAAAT
    GCTAATGTAA ACCTAACTAA TGTCAGACTA GAGGAGCCTA CTGCTGCTCC TTACAACTCT
    TATCAACCAC AAGCGGGCCC CTTAAGAACA TCAAGTACTG AGGGAGCTCA TGTAGTGCTA
    AGAGATCAAG AGCCATCATC CTTACCGCCG CATATAGATC CAGCAAAGGT ATACAGAAAG
    GATCCATATA TTAGTGAAGA AGCATCCAGA CAAAGCTATG TCTTAAAACA GCCAGCTGTT
    AATCACCCAG TACAAAGACA GGAAAGAGAC CCAAATCTGA TCTATGAATC TCAGACTCAG
    TATGCAGAAA AACAACCAAG TAGGGAGTAC GAGCAGTCAA CATACAGGTA TGATTCTACA
    AACTATGTAG ATCAATTTTC TCGTGGGTAT GACCCTCGTC TACATTATGA AGATCGTGCG
    CCTCCCTATG AGGAGCACTG GTCATATTAT GATGAAAAAC AGCCCTACAA TCAACCAAGA
    ACAGCTTATG AAAGCCAGCC TCCTAGAGAT CTTGATTCCA GACAAAATAC AGAAGAGAGC
    ACAGAACGCA GCTATTATCC GGCACAACCC CGATTTGAGG AACCTCCTCC GATGAGCTAC
    GATGGCAGAC CTCGCTATGA GCATGCACCC AAAAACTTCA GCTTGCCACA AGTGCGGTAT
    GAAGATCAAC ATCCTGTTGG CTATGACACA CATGCGCATG GTAGATATAA ACAAGAAGCT
    CAGCCATACC AGTCAGCCAT ATCTCGATCT CCTGAACCCA AGCAGTACTT TGATCCACAT
    GTGAGGGGCT ATGAGCAAGG TCCTCCTCAA GCTTACAATG CAAAAGCTGG ACAGTTTGAG
    CCTTCTCATA GCACTTCAGG TGTTTCTCTT CCTCCTCCCG CTTCTTCACA AACAAAACCA
    GAAGTTTTGC CTTCCAACAG TAAACCACTG CCTACACCAC CATCTCTAGC AGAAGAAGAG
    GAGGATCCAG CCATGAAACC ACAGTCTGTG CGAAGTAGAG TTAAGATATT TGAACGAAGA
    AGATCGCCAT CTCTGGAAAA AATGAAGGAC CCCAGTGACA CGTCAGTTGT CAAACCTCCA
    GAGCTAGCAC CGAAGCCTAC GCTCCCAGCC ATGAGTGGTC CAAAACCAAC TTCTCAAAGC
    CAGTATGAAC ATGATAAAAC AACTTACAGG GCCCCAGAAC CACAGAGACC TCAAGTGAAG
    CCACCTGAAG ATATCGTGCG TTCAAATCAT TATGATCCCG AAGAAGATGA AGAGTATTAC
    AGAAAGCAGC TCTCATACTT TGATCGCAGG AATTTTGAAA ATAAGCCTTC TGCACAGGTT
    CCTGCCAGCC ATCATTCTGA CTCCACTAAA CCAATACATT CACAAAATCA GCTGAATTTC
    TCCAATTATT CTAAGGGGAA GCCAACTGAT GCTGAACCAA TGGATAGATC TGTTGGTGAC
    AAGCGCTATG AGCCAATCCC TCAAGTTACA ACTCCTCCTC CTGCTCCTTC AGTACAGTAT
    ACACAACCTC AGTCAATTAA TAGTCCTGTC CTGTCTCTCC CAGCGCATCA CAAGCCTGCA
    CTTTCTGAAG TTAACTCTGT GTCTGACCCT CCTCCACCTC AGAATAAGCC AGCAATTTTC
    AGATCCTCTA GAGAGGACAC TGTGCAGTCT ACTTTTTATC CTCAGAAAAG CTTCCCTGAC
    AAAGGTCCTG TTAATGGAAC TGAACAGGTT CAGAAAACAG TCACTCCTTC TTACAACCGT
    TTTACAACAA AACCTTACAC TAGTGCTGCG AGGCCCTTTG AGCGCAAGTT TGAAAGTCCT
    AAATTCAACC ATAATCTCTT GCCAAATGAA GCTCAACATA AACCAGAGTT GCCATCAAAA
    TCTCCAAATT CTCCTCAACC AATTTTGAAA GCACACAGCT CATCACAGCC TCCTGAGTTT
    GACAGTGGAA TGGACACCTT CACTGTACAG GTTGACAAGC CTAAATATCA ACCAAATAAT
    GTAAATGCTG TGCCTAAAGC CATTCCTGTA AGCCCTTCAG CACTAGAGGA TGAGGAGGAA
    GAAGACGGAC ACACTGTTGT AGCCACAGCA AGAGGTGTAT TCAACAGCAA TGGTGGCGTA
    TTGAGCTCCA TAGAGACTGG AGTTAGTATT ATTATACCAC AAGGAGCCAT TCCAGAGGGA
    ATAGAGCAAG AAATATATTT TAAAGTCTGC AGAGACAACA GTATACTTCC ACCTTTGGAC
    AAAGAGAAAG GTGAAACACT TCTTAGCCCC TTGGTAATGT GTGGGCCTCA CGGACTAAAA
    TTCCTGAAGC CAGTGGAGCT GCGCCTGCCA CACTGTGCGT CTATGACCCC TGATGGTGAC
    CCCAAAACCT GGCAGAACAA GTCTCTTCCT GGAGATCCAA ACTATCTTGT TGGAGCAAAC
    TGTGTCTCAG TCCTAATTGA CCACTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015134462.2
    CDS16..5565
    Translation

    Target ORF information:

    RefSeq Version XM_015278976.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus tight junction protein 1 (TJP1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015278976.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCAGCGGC TGGAGAGACT CTGTCGGTGG CGGCGGCGGG GACGCGGCAG GGACAGGGGG 
    GGCGACGGCA AAATGAAGTA TCAGAAATAC CTGACTGTCT TGCAGATGGC CATAGGGGTG
    ACCGCCTCCA ACAGGGGCAG TCTGATGCCC TTAAAGAGGA AGCTGTGGGT AACTCCATCT
    TCCGAAAATC CCAATGGTGC TACCTGTAGT GTAAGTCAGG GAAAGCCTTC TTTAAGAAGA
    ATAAAAGGGA GAATACACAG AAGCAAAAGC CTTGATAGCA TTGATTTCTG TGAATTCACT
    AGTGCAACAA TGGAAGAAAC AGCTATATGG GAACAACACA CGGTGACTCT TCACAGGGCT
    CCTGGATTTG GGTTTGGTAT TGCAATATCT GGAGGAAGGG ATAATCCTCA CTTTCAAAGT
    GGTGAGACCT CAATAGTTAT TTCTGATGTG CTGAAAGGAG GCCCAGCAGA AGGACTGCTA
    CAAGAAAATG ACCGTGTTGC AATGGTTAAT GGAGTTTCAA TGGATAATGT TGAGCACGCA
    TTTGCTGTTC AGCAGCTGAG GAAAAGTGGG AAGAATGCCA AAATTACTAT CCGAAGGATG
    AAGAAAATTC AGATTCCAGT AGCTCGACAG GAACCTGAGC CAGTGTCTGA AAATGAGGAC
    GATAGCTATG ATGAAGAGAT ACGGGATCCA AGGACCAGCC GTGGTGCTTC CAGTGCCAAC
    AGAAGGCATG AGAAGAGCTG GGTACGAGAC CGAAGTGCAA GCAGAGAAAG AAGCTTATCA
    CCAAGATCTG ACAGAAGATC GGTTACTTCC AGTCAGCCTG CAAAACCTAC CAAAGTAACG
    TTGGTGAAAT CAAGGAAAAA TGAAGAGTAT GGTCTACGGT TGGCAAGCCA CATATTTGTG
    AAAGAAATAT CACAGGATAG CCTTGCAGCA AGAGATGGCA ATATTCAGGA AGGGGATGTT
    GTTCTGAAGA TAAATGGCAC AGTGACAGAA AATATGTCCT TAGCAGATGC AAAAACGCTA
    ATAGAAAGGT CCAAAGGCAA GTTGAAGATG GTGGTTCAGC GAGATGAACG AGCTACACTG
    TTGAATGTCC CTGATCTTTC TGACAGTATT CATTCTGCTA ATGCTTCGGA AAGAGATGAC
    ATCTCAGAAA TTCAGTCGCT GGCATCAGAT CATTCCAACC GATCACATGA CAGGCCCCGC
    CGCAGTCGTT CACGATCTCC TGACCAGAGG TCAGAGCCTT CAGACCATTC CAGACATTCT
    CCACAGCAGC CCAGCAACGG CAGTCTTCGA AGCAGAGAAG ATGAAAGGGT AACTAAACCA
    GGAGCCATCT CTACACCTGT AAAGAATGCA GATGATATTT CTAAAACTAT GGAAGAGGTG
    TCAGTCGAAA GAACTGAAAA ACAAACACCA CCGCTCCCAG AACCAAAGCC AGTATATGCA
    CAAGGAGGTC AGCCAGATGT GGATTTACCA GTCAGTCCGT CTGATGGTCC TTTACCCAAT
    TCAACCCATG AAGATGGAAT GCTTCGGCCA AGCATGAAAC TGGTAAAATT CAGAAAAGGA
    GATAGTGTGG GCCTGCGGCT CGCTGGTGGC AATGATGTTG GCATATTTGT AGCTGGTGTT
    CTAGAGGACA GCCCTGCAGC GAAAGAAGGA TTAGAGGAAG GAGACCAAAT TCTCAGGGTT
    AATAACGTAG ACTTCACAAA TATCATCAGA GAAGAAGCTG TCCTTTTTTT GCTTGATCTC
    CCTAAAGGCG AAGAAGTAAC CATTTTGGCA CAGAAGAAGA AAGATGTTTA TCGTCGCATT
    GTTGAGTCTG ATGTAGGGGA TTCTTTCTAT ATCAGAACTC ATTTTGAATA TGAAAAGGAA
    TCTCCTTATG GACTAAGCTT CAACAAAGGT GAAGTGTTTC GGGTTGTGGA CACGCTATAT
    AATGGCAAAC TGGGTTCGTG GCTGGCAATT CGAATTGGCA AGAATCATAA GGAAGTTGAA
    AGAGGTATCA TACCTAACAA AAACAGAGCT GAACAACTAG CTAGTGTACA GTACACGCTT
    CCAAAGACAG CAGGAGGAGA TCGAGCGGAT TTCTGGAGAT TCCGAGGTTT GCGTAGCTCA
    AAAAGAAATC TCCGAAAAAG TAGAGAAGAT CTTTCTGCTC AGCCTGTTCA GACAAAGTTC
    CCTGCCTATG AAAGAGTTGT TCTTCGGGAA GCTGGGTTTC TCAGACCTGT GACCATTTTT
    GGTCCAATAG CTGATGTTGC ACGAGAGAAA CTAGCCAGAG AAGAACCAGA TATCTTTCAA
    ATTGCAAAAA GTGAACCGAG AGATGCTGGT ACTGACCAAC GTAGTTCTGG CATTATTCGT
    CTTCATACGA TAAAACAGAT AATTGATAGA GACAAACACG CTTTATTAGA TGTCACTCCT
    AATGCAGTGG ACCGTCTGAA TTATGCACAG TGGTATCCTA TTGTAGTGTT TCTGAACCCT
    GATTCTAAGC AGGGTGTGAA GACCATGAGA ATGAGGCTGT GCCCAGAATC ACGAAAAAGT
    GCCAGGAAGC TATATGAAAG AGCTCACAAG CTACGCAAAA ATAATCACCA CCTCTTTACA
    ACTACCATTA ACTTGAATTC AATGAATGAA GGATGGTATG GAGCTCTAAA AGAAGCAATT
    CAGCAACAAC AAAACCAACT GGTGTGGGTT TCTGAAGGAA AGGCAGATGG TGCTACAAGT
    GATGACCTTG ATTTACATGA TGACCGCCTG TCTTACCTCT CAGCTCCTGG TAGTGAATAT
    TCTATGTACA GTACAGACAG CAGACATACA TCTGACTATG AAGACACAGA TACAGAAGGT
    GGTGCTTATA CTGATCAAGA ACTGGATGAA ACTCTGAACG ATGAGGTTGG AACTCCTCCT
    GAATCTGCTA TTACACGTTC TTCTGAACCC GTTAGGGAGG ATTCTTCTGG AATGCATCAT
    GAAACTCAAA CTTACACTAG TTACGCATCT CAAGCTCAGC CTCAGCCAAA TCTTAGAATA
    GATTCTTCAG GATTTAAAAC AACTGCTTCT CAGCCGAAAG CAGAAGCATC ACCTGCAGTC
    CCTTACCTTT CCCCGTCGCC TGAATCAAAC CCAGCAACCT CATCAACCTC TGCAGTAAAT
    GCTAATGTAA ACCTAACTAA TGTCAGACTA GAGGAGCCTA CTGCTGCTCC TTACAACTCT
    TATCAACCAC AAGCGGGCCC CTTAAGAACA TCAAGTACTG AGGGAGCTCA TGTAGTGCTA
    AGAGATCAAG AGCCATCATC CTTACCGCCG CATATAGATC CAGCAAAGGT ATACAGAAAG
    GATCCATATA TTAGTGAAGA AGCATCCAGA CAAAGCTATG TCTTAAAACA GCCAGCTGTT
    AATCACCCAG TACAAAGACA GGAAAGAGAC CCAAATCTGA TCTATGAATC TCAGACTCAG
    TATGCAGAAA AACAACCAAG TAGGGAGTAC GAGCAGTCAA CATACAGGTA TGATTCTACA
    AACTATGTAG ATCAATTTTC TCGTGGGTAT GACCCTCGTC TACATTATGA AGATCGTGCG
    CCTCCCTATG AGGAGCACTG GTCATATTAT GATGAAAAAC AGCCCTACAA TCAACCAAGA
    ACAGCTTATG AAAGCCAGCC TCCTAGAGAT CTTGATTCCA GACAAAATAC AGAAGAGAGC
    ACAGAACGCA GCTATTATCC GGCACAACCC CGATTTGAGG AACCTCCTCC GATGAGCTAC
    GATGGCAGAC CTCGCTATGA GCATGCACCC AAAAACTTCA GCTTGCCACA AGTGCGGTAT
    GAAGATCAAC ATCCTGTTGG CTATGACACA CATGCGCATG GTAGATATAA ACAAGAAGCT
    CAGCCATACC AGTCAGCCAT ATCTCGATCT CCTGAACCCA AGCAGTACTT TGATCCACAT
    GTGAGGGGCT ATGAGCAAGG TCCTCCTCAA GCTTACAATG CAAAAGCTGG ACAGTTTGAG
    CCTTCTCATA GCACTTCAGG TGTTTCTCTT CCTCCTCCCG CTTCTTCACA AACAAAACCA
    GAAGTTTTGC CTTCCAACAG TAAACCACTG CCTACACCAC CATCTCTAGC AGAAGAAGAG
    GAGGATCCAG CCATGAAACC ACAGTCTGTG CGAAGTAGAG TTAAGATATT TGAACGAAGA
    AGATCGCCAT CTCTGGAAAA AATGAAGGAC CCCAGTGACA CGTCAGTTGT CAAACCTCCA
    GAGCTAGCAC CGAAGCCTAC GCTCCCAGCC ATGAGTGGTC CAAAACCAAC TTCTCAAAGC
    CAGTATGAAC ATGATAAAAC AACTTACAGG GCCCCAGAAC CACAGAGACC TCAAGTGAAG
    CCACCTGAAG ATATCGTGCG TTCAAATCAT TATGATCCCG AAGAAGATGA AGAGTATTAC
    AGAAAGCAGC TCTCATACTT TGATCGCAGG AATTTTGAAA ATAAGCCTTC TGCACAGGTT
    CCTGCCAGCC ATCATTCTGA CTCCACTAAA CCAATACATT CACAAAATCA GCTGAATTTC
    TCCAATTATT CTAAGGGGAA GCCAACTGAT GCTGAACCAA TGGATAGATC TGTTGGTGAC
    AAGCGCTATG AGCCAATCCC TCAAGTTACA ACTCCTCCTC CTGCTCCTTC AGTACAGTAT
    ACACAACCTC AGTCAATTAA TAGTCCTGTC CTGTCTCTCC CAGCGCATCA CAAGCCTGCA
    CTTTCTGAAG TTAACTCTGT GTCTGACCCT CCTCCACCTC AGAATAAGCC AGCAATTTTC
    AGATCCTCTA GAGAGGACAC TGTGCAGTCT ACTTTTTATC CTCAGAAAAG CTTCCCTGAC
    AAAGGTCCTG TTAATGGAAC TGAACAGGTT CAGAAAACAG TCACTCCTTC TTACAACCGT
    TTTACAACAA AACCTTACAC TAGTGCTGCG AGGCCCTTTG AGCGCAAGTT TGAAAGTCCT
    AAATTCAACC ATAATCTCTT GCCAAATGAA GCTCAACATA AACCAGAGTT GCCATCAAAA
    TCTCCAAATT CTCCTCAACC AATTTTGAAA GCACACAGCT CATCACAGCC TCCTGAGTTT
    GACAGTGGAA TGGACACCTT CACTGTACAG GTTGACAAGC CTAAATATCA ACCAAATAAT
    GTAAATGCTG TGCCTAAAGC CATTCCTGTA AGCCCTTCAG CACTAGAGGA TGAGGAGGAA
    GAAGACGGAC ACACTGTTGT AGCCACAGCA AGAGGTGTAT TCAACAGCAA TGGTGGCGTA
    TTGAGCTCCA TAGAGACTGG AGTTAGTATT ATTATACCAC AAGGAGCCAT TCCAGAGGGA
    ATAGAGCAAG AAATATATTT TAAAGTCTGC AGAGACAACA GTATACTTCC ACCTTTGGAC
    AAAGAGAAAG GTGAAACACT TCTTAGCCCC TTGGTAATGT GTGGGCCTCA CGGACTAAAA
    TTCCTGAAGC CAGTGGAGCT GCGCCTGCCA CACTGTGCGT CTATGACCCC TGATGGTGAC
    CCCAAAACCT GGCAGAACAA GTCTCTTCCT GGAGATCCAA ACTATCTTGT TGGAGCAAAC
    TGTGTCTCAG TCCTAATTGA CCACTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa143494
    Clone ID Related Accession (Same CDS sequence) XM_015278977.2
    Accession Version XM_015278977.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5529bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product tight junction protein ZO-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006097.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015278977.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCAGCGGC TGGAGAGACT CTGTCGGTGG CGGCGGCGGG GACGCGGCAG GGACAGGGGG 
    GGCGACGGCA AAATGAAGTA TCAGAAATAC CTGACTGTCT TGCAGATGGC CATAGGGGTG
    ACCGCCTCCA ACAGGGGCAG TCTGATGCCC TTAAAGAGGA AGCTGTGGGT AACTCCATCT
    TCCGAAAATC CCAATGGTGC TACCTGTAGT GTAAGTCAGG GAAAGCCTTC TTTAAGAAGA
    ATAAAAGGGA GAATACACAG AAGCAAAAGC CTTGATAGCA TTGATTTCTG TGAATTCACT
    AGTGCAACAA TGGAAGAAAC AGCTATATGG GAACAACACA CGGTGACTCT TCACAGGGCT
    CCTGGATTTG GGTTTGGTAT TGCAATATCT GGAGGAAGGG ATAATCCTCA CTTTCAAAGT
    GGTGAGACCT CAATAGTTAT TTCTGATGTG CTGAAAGGAG GCCCAGCAGA AGGACTGCTA
    CAAGAAAATG ACCGTGTTGC AATGGTTAAT GGAGTTTCAA TGGATAATGT TGAGCACGCA
    TTTGCTGTTC AGCAGCTGAG GAAAAGTGGG AAGAATGCCA AAATTACTAT CCGAAGGATG
    AAGAAAATTC AGATTCCAGT AGCTCGACAG GAACCTGAGC CAGTGTCTGA AAATGAGGAC
    GATAGCTATG ATGAAGAGAT ACGGGATCCA AGGACCAGCC GTGGTGCTTC CAGTGCCAAC
    AGAAGGCATG AGAAGAGCTG GGTACGAGAC CGAAGTGCAA GCAGAGAAAG AAGCTTATCA
    CCAAGATCTG ACAGAAGATC GGTTACTTCC AGTCAGCCTG CAAAACCTAC CAAAGTAACG
    TTGGTGAAAT CAAGGAAAAA TGAAGAGTAT GGTCTACGGT TGGCAAGCCA CATATTTGTG
    AAAGAAATAT CACAGGATAG CCTTGCAGCA AGAGATGGCA ATATTCAGGA AGGGGATGTT
    GTTCTGAAGA TAAATGGCAC AGTGACAGAA AATATGTCCT TAGCAGATGC AAAAACGCTA
    ATAGAAAGGT CCAAAGGCAA GTTGAAGATG GTGGTTCAGC GAGATGAACG AGCTACACTG
    TTGAATGTCC CTGATCTTTC TGACAGTATT CATTCTGCTA ATGCTTCGGA AAGAGATGAC
    ATCTCAGAAA TTCAGTCGCT GGCATCAGAT CATTCCAACC GATCACATGA CAGGCCCCGC
    CGCAGTCGTT CACGATCTCC TGACCAGAGG TCAGAGCCTT CAGACCATTC CAGACATTCT
    CCACAGCAGC CCAGCAACGG CAGTCTTCGA AGCAGAGAAG ATGAAAGGGT AACTAAACCA
    GGAGCCATCT CTACACCTGT AAAGAATGCA GATGATATTT CTAAAACTAT GGAAGAGGTG
    TCAGTCGAAA GAACTGAAAA ACAAACACCA CCGCTCCCAG AACCAAAGCC AGTATATGCA
    CAAGGAGGTC AGCCAGATGT GGATTTACCA GTCAGTCCGT CTGATGGTCC TTTACCCAAT
    TCAACCCATG AAGATGGAAT GCTTCGGCCA AGCATGAAAC TGGTAAAATT CAGAAAAGGA
    GATAGTGTGG GCCTGCGGCT CGCTGGTGGC AATGATGTTG GCATATTTGT AGCTGGTGTT
    CTAGAGGACA GCCCTGCAGC GAAAGAAGGA TTAGAGGAAG GAGACCAAAT TCTCAGGGTT
    AATAACGTAG ACTTCACAAA TATCATCAGA GAAGAAGCTG TCCTTTTTTT GCTTGATCTC
    CCTAAAGGCG AAGAAGTAAC CATTTTGGCA CAGAAGAAGA AAGATGTTTA TCGTCGCATT
    GTTGAGTCTG ATGTAGGGGA TTCTTTCTAT ATCAGAACTC ATTTTGAATA TGAAAAGGAA
    TCTCCTTATG GACTAAGCTT CAACAAAGGT GAAGTGTTTC GGGTTGTGGA CACGCTATAT
    AATGGCAAAC TGGGTTCGTG GCTGGCAATT CGAATTGGCA AGAATCATAA GGAAGTTGAA
    AGAGGTATCA TACCTAACAA AAACAGAGCT GAACAACTAG CTAGTGTACA GTACACGCTT
    CCAAAGACAG CAGGAGGAGA TCGAGCGGAT TTCTGGAGAT TCCGAGGTTT GCGTAGCTCA
    AAAAGAAATC TCCGAAAAAG TAGAGAAGAT CTTTCTGCTC AGCCTGTTCA GACAAAGTTC
    CCTGCCTATG AAAGAGTTGT TCTTCGGGAA GCTGGGTTTC TCAGACCTGT GACCATTTTT
    GGTCCAATAG CTGATGTTGC ACGAGAGAAA CTAGCCAGAG AAGAACCAGA TATCTTTCAA
    ATTGCAAAAA GTGAACCGAG AGATGCTGGT ACTGACCAAC GTAGTTCTGG CATTATTCGT
    CTTCATACGA TAAAACAGAT AATTGATAGA GACAAACACG CTTTATTAGA TGTCACTCCT
    AATGCAGTGG ACCGTCTGAA TTATGCACAG TGGTATCCTA TTGTAGTGTT TCTGAACCCT
    GATTCTAAGC AGGGTGTGAA GACCATGAGA ATGAGGCTGT GCCCAGAATC ACGAAAAAGT
    GCCAGGAAGC TATATGAAAG AGCTCACAAG CTACGCAAAA ATAATCACCA CCTCTTTACA
    ACTACCATTA ACTTGAATTC AATGAATGAA GGATGGTATG GAGCTCTAAA AGAAGCAATT
    CAGCAACAAC AAAACCAACT GGTGTGGGTT TCTGAAGGAA AGGCAGATGG TGCTACAAGT
    GATGACCTTG ATTTACATGA TGACCGCCTG TCTTACCTCT CAGCTCCTGG TAGTGAATAT
    TCTATGTACA GTACAGACAG CAGACATACA TCTGACTATG AAGACACAGA TACAGAAGGT
    GGTGCTTATA CTGATCAAGA ACTGGATGAA ACTCTGAACG ATGAGGTTGG AACTCCTCCT
    GAATCTGCTA TTACACGTTC TTCTGAACCC GTTAGGGAGG ATTCTTCTGG AATGCATCAT
    GAAACTCAAA CTTACACTAG TTACGCATCT CAAGCTCAGC CTCAGCCAAA TCTTAGAATA
    GATTCTTCAG GATTTAAAAC AACTGCTTCT CAGCCGAAAG CAGAAGCATC ACCTGCAGTC
    CCTTACCTTT CCCCGTCGCC TGAATCAAAC CCAGCAACCT CATCAACCTC TGCAGTAAAT
    GCTAATGTAA ACCTAACTAA TGTCAGACTA GAGGAGCCTA CTGCTGCTCC TTACAACTCT
    TATCAACCAC AAGCGGGCCC CTTAAGAACA TCAAGTACTG AGGGAGCTCA TGTAGTGCTA
    AGAGATCAAG AGCCATCATC CTTACCGCCG CATATAGATC CAGCAAAGGT ATACAGAAAG
    GATCCATATA TTAGTGAAGA AGCATCCAGA CAAAGCTATG TCTTAAAACA GCCAGCTGTT
    AATCACCCAG TACAAAGACA GGAAAGAGAC CCAAATCTGA TCTATGAATC TCAGACTCAG
    TATGCAGAAA AACAACCAAG TAGGGAGTAC GAGCAGTCAA CATACAGGTA TGATTCTACA
    AACTATGTAG ATCAATTTTC TCGTGGGTAT GACCCTCGTC TACATTATGA AGATCGTGCG
    CCTCCCTATG AGGAGCACTG GTCATATTAT GATGAAAAAC AGCCCTACAA TCAACCAAGA
    ACAGCTTATG AAAGCCAGCC TCCTAGAGAT CTTGATTCCA GACAAAATAC AGAAGAGAGC
    ACAGAACGCA GCTATTATCC GGCACAACCC CGATTTGAGG AACCTCCTCC GATGAGCTAC
    GATGGCAGAC CTCGCTATGA GCATGCACCC AAAAACTTCA GCTTGCCACA AGTGCGGTAT
    GAAGATCAAC ATCCTGTTGG CTATGACACA CATGCGCATG GTAGATATAA ACAAGAAGCT
    CAGCCATACC AGTCAGCCAT ATCTCGATCT CCTGAACCCA AGCAGTACTT TGATCCACAT
    GTGAGGGGCT ATGAGCAAGG TCCTCCTCAA GCTTACAATG CAAAAGCTGG ACAGTTTGAG
    CCTTCTCATA GCACTTCAGG TGTTTCTCTT CCTCCTCCCG CTTCTTCACA AACAAAACCA
    GAAGTTTTGC CTTCCAACAG TAAACCACTG CCTACACCAC CATCTCTAGC AGAAGAAGAG
    GAGGATCCAG CCATGAAACC ACAGTCTGTG CGAAGTAGAG TTAAGATATT TGAACGAAGA
    AGATCGCCAT CTCTGGAAAA AATGAAGGAC CCCAGTGACA CGTCAGTTGT CAAACCTCCA
    GAGCTAGCAC CGAAGCCTAC GCTCCCAGCC ATGAGTGGTC CAAAACCAAC TTCTCAAAGC
    CAGTATGAAC ATGATAAAAC AACTTACAGG GCCCCAGAAC CACAGAGACC TCAAGTGAAG
    CCACCTGAAG ATATCGTGCG TTCAAATCAT TATGATCCCG AAGAAGATGA AGAGTATTAC
    AGAAAGCAGC TCTCATACTT TGATCGCAGG AATTTTGAAA ATAAGCCTTC TGCACAGGTT
    CCTGCCAGCC ATCATTCTGA CTCCACTAAA CCAATACATT CACAAAATCA GCTGAATTTC
    TCCAATTATT CTAAGGGGAA GCCAACTGAT GCTGAACCAA TGGATAGATC TGTTGGTGAC
    AAGCGCTATG AGCCAATCCC TCAAGTTACA ACTCCTCCTC CTGCTCCTTC AGTACAGTAT
    ACACAACCTC AGTCAATTAA TAGTCCTGTC CTGTCTCTCC CAGCGCATCA CAAGCCTGCA
    CTTTCTGAAG TTAACTCTGT GTCTGACCCT CCTCCACCTC AGAATAAGCC AGCAATTTTC
    AGATCCTCTA GAGAGGACAC TGTGCAGTCT ACTTTTTATC CTCAGAAAAG CTTCCCTGAC
    AAAGGTCCTG TTAATGGAAC TGAACAGGTT CAGAAAACAG TCACTCCTTC TTACAACCGT
    TTTACAACAA AACCTTACAC TAGTGCTGCG AGGCCCTTTG AGCGCAAGTT TGAAAGTCCT
    AAATTCAACC ATAATCTCTT GCCAAATGAA GCTCAACATA AACCAGAGTT GCCATCAAAA
    TCTCCAAATT CTCCTCAACC AATTTTGAAA GCACACAGCT CATCACAGCC TCCTGAGTTT
    GACAGTGGAA TGGACACCTT CACTGTACAG GTTGACAAGC CTAAATATCA ACCAAATAAT
    GTAAATGCTG TGCCTAAAGC CATTCCTGTA AGCCCTTCAG CACTAGAGGA TGAGGAGGAA
    GAAGACGGAC ACACTGTTGT AGCCACAGCA AGAGGTGTAT TCAACAGCAA TGGTGGCGTA
    TTGAGCTCCA TAGAGACTGG AGTTAGTATT ATTATACCAC AAGGAGCCAT TCCAGAGGGA
    ATAGAGCAAG AAATATATTT TAAAGTCTGC AGAGACAACA GTATACTTCC ACCTTTGGAC
    AAAGAGAAAG GTGAAACACT TCTTAGCCCC TTGGTAATGT GTGGGCCTCA CGGACTAAAA
    TTCCTGAAGC CAGTGGAGCT GCGCCTGCCA CACTGTGACC CCAAAACCTG GCAGAACAAG
    TCTCTTCCTG GAGATCCAAA CTATCTTGTT GGAGCAAACT GTGTCTCAGT CCTAATTGAC
    CACTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015134463.2
    CDS16..5544
    Translation

    Target ORF information:

    RefSeq Version XM_015278977.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus tight junction protein 1 (TJP1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015278977.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCAGCGGC TGGAGAGACT CTGTCGGTGG CGGCGGCGGG GACGCGGCAG GGACAGGGGG 
    GGCGACGGCA AAATGAAGTA TCAGAAATAC CTGACTGTCT TGCAGATGGC CATAGGGGTG
    ACCGCCTCCA ACAGGGGCAG TCTGATGCCC TTAAAGAGGA AGCTGTGGGT AACTCCATCT
    TCCGAAAATC CCAATGGTGC TACCTGTAGT GTAAGTCAGG GAAAGCCTTC TTTAAGAAGA
    ATAAAAGGGA GAATACACAG AAGCAAAAGC CTTGATAGCA TTGATTTCTG TGAATTCACT
    AGTGCAACAA TGGAAGAAAC AGCTATATGG GAACAACACA CGGTGACTCT TCACAGGGCT
    CCTGGATTTG GGTTTGGTAT TGCAATATCT GGAGGAAGGG ATAATCCTCA CTTTCAAAGT
    GGTGAGACCT CAATAGTTAT TTCTGATGTG CTGAAAGGAG GCCCAGCAGA AGGACTGCTA
    CAAGAAAATG ACCGTGTTGC AATGGTTAAT GGAGTTTCAA TGGATAATGT TGAGCACGCA
    TTTGCTGTTC AGCAGCTGAG GAAAAGTGGG AAGAATGCCA AAATTACTAT CCGAAGGATG
    AAGAAAATTC AGATTCCAGT AGCTCGACAG GAACCTGAGC CAGTGTCTGA AAATGAGGAC
    GATAGCTATG ATGAAGAGAT ACGGGATCCA AGGACCAGCC GTGGTGCTTC CAGTGCCAAC
    AGAAGGCATG AGAAGAGCTG GGTACGAGAC CGAAGTGCAA GCAGAGAAAG AAGCTTATCA
    CCAAGATCTG ACAGAAGATC GGTTACTTCC AGTCAGCCTG CAAAACCTAC CAAAGTAACG
    TTGGTGAAAT CAAGGAAAAA TGAAGAGTAT GGTCTACGGT TGGCAAGCCA CATATTTGTG
    AAAGAAATAT CACAGGATAG CCTTGCAGCA AGAGATGGCA ATATTCAGGA AGGGGATGTT
    GTTCTGAAGA TAAATGGCAC AGTGACAGAA AATATGTCCT TAGCAGATGC AAAAACGCTA
    ATAGAAAGGT CCAAAGGCAA GTTGAAGATG GTGGTTCAGC GAGATGAACG AGCTACACTG
    TTGAATGTCC CTGATCTTTC TGACAGTATT CATTCTGCTA ATGCTTCGGA AAGAGATGAC
    ATCTCAGAAA TTCAGTCGCT GGCATCAGAT CATTCCAACC GATCACATGA CAGGCCCCGC
    CGCAGTCGTT CACGATCTCC TGACCAGAGG TCAGAGCCTT CAGACCATTC CAGACATTCT
    CCACAGCAGC CCAGCAACGG CAGTCTTCGA AGCAGAGAAG ATGAAAGGGT AACTAAACCA
    GGAGCCATCT CTACACCTGT AAAGAATGCA GATGATATTT CTAAAACTAT GGAAGAGGTG
    TCAGTCGAAA GAACTGAAAA ACAAACACCA CCGCTCCCAG AACCAAAGCC AGTATATGCA
    CAAGGAGGTC AGCCAGATGT GGATTTACCA GTCAGTCCGT CTGATGGTCC TTTACCCAAT
    TCAACCCATG AAGATGGAAT GCTTCGGCCA AGCATGAAAC TGGTAAAATT CAGAAAAGGA
    GATAGTGTGG GCCTGCGGCT CGCTGGTGGC AATGATGTTG GCATATTTGT AGCTGGTGTT
    CTAGAGGACA GCCCTGCAGC GAAAGAAGGA TTAGAGGAAG GAGACCAAAT TCTCAGGGTT
    AATAACGTAG ACTTCACAAA TATCATCAGA GAAGAAGCTG TCCTTTTTTT GCTTGATCTC
    CCTAAAGGCG AAGAAGTAAC CATTTTGGCA CAGAAGAAGA AAGATGTTTA TCGTCGCATT
    GTTGAGTCTG ATGTAGGGGA TTCTTTCTAT ATCAGAACTC ATTTTGAATA TGAAAAGGAA
    TCTCCTTATG GACTAAGCTT CAACAAAGGT GAAGTGTTTC GGGTTGTGGA CACGCTATAT
    AATGGCAAAC TGGGTTCGTG GCTGGCAATT CGAATTGGCA AGAATCATAA GGAAGTTGAA
    AGAGGTATCA TACCTAACAA AAACAGAGCT GAACAACTAG CTAGTGTACA GTACACGCTT
    CCAAAGACAG CAGGAGGAGA TCGAGCGGAT TTCTGGAGAT TCCGAGGTTT GCGTAGCTCA
    AAAAGAAATC TCCGAAAAAG TAGAGAAGAT CTTTCTGCTC AGCCTGTTCA GACAAAGTTC
    CCTGCCTATG AAAGAGTTGT TCTTCGGGAA GCTGGGTTTC TCAGACCTGT GACCATTTTT
    GGTCCAATAG CTGATGTTGC ACGAGAGAAA CTAGCCAGAG AAGAACCAGA TATCTTTCAA
    ATTGCAAAAA GTGAACCGAG AGATGCTGGT ACTGACCAAC GTAGTTCTGG CATTATTCGT
    CTTCATACGA TAAAACAGAT AATTGATAGA GACAAACACG CTTTATTAGA TGTCACTCCT
    AATGCAGTGG ACCGTCTGAA TTATGCACAG TGGTATCCTA TTGTAGTGTT TCTGAACCCT
    GATTCTAAGC AGGGTGTGAA GACCATGAGA ATGAGGCTGT GCCCAGAATC ACGAAAAAGT
    GCCAGGAAGC TATATGAAAG AGCTCACAAG CTACGCAAAA ATAATCACCA CCTCTTTACA
    ACTACCATTA ACTTGAATTC AATGAATGAA GGATGGTATG GAGCTCTAAA AGAAGCAATT
    CAGCAACAAC AAAACCAACT GGTGTGGGTT TCTGAAGGAA AGGCAGATGG TGCTACAAGT
    GATGACCTTG ATTTACATGA TGACCGCCTG TCTTACCTCT CAGCTCCTGG TAGTGAATAT
    TCTATGTACA GTACAGACAG CAGACATACA TCTGACTATG AAGACACAGA TACAGAAGGT
    GGTGCTTATA CTGATCAAGA ACTGGATGAA ACTCTGAACG ATGAGGTTGG AACTCCTCCT
    GAATCTGCTA TTACACGTTC TTCTGAACCC GTTAGGGAGG ATTCTTCTGG AATGCATCAT
    GAAACTCAAA CTTACACTAG TTACGCATCT CAAGCTCAGC CTCAGCCAAA TCTTAGAATA
    GATTCTTCAG GATTTAAAAC AACTGCTTCT CAGCCGAAAG CAGAAGCATC ACCTGCAGTC
    CCTTACCTTT CCCCGTCGCC TGAATCAAAC CCAGCAACCT CATCAACCTC TGCAGTAAAT
    GCTAATGTAA ACCTAACTAA TGTCAGACTA GAGGAGCCTA CTGCTGCTCC TTACAACTCT
    TATCAACCAC AAGCGGGCCC CTTAAGAACA TCAAGTACTG AGGGAGCTCA TGTAGTGCTA
    AGAGATCAAG AGCCATCATC CTTACCGCCG CATATAGATC CAGCAAAGGT ATACAGAAAG
    GATCCATATA TTAGTGAAGA AGCATCCAGA CAAAGCTATG TCTTAAAACA GCCAGCTGTT
    AATCACCCAG TACAAAGACA GGAAAGAGAC CCAAATCTGA TCTATGAATC TCAGACTCAG
    TATGCAGAAA AACAACCAAG TAGGGAGTAC GAGCAGTCAA CATACAGGTA TGATTCTACA
    AACTATGTAG ATCAATTTTC TCGTGGGTAT GACCCTCGTC TACATTATGA AGATCGTGCG
    CCTCCCTATG AGGAGCACTG GTCATATTAT GATGAAAAAC AGCCCTACAA TCAACCAAGA
    ACAGCTTATG AAAGCCAGCC TCCTAGAGAT CTTGATTCCA GACAAAATAC AGAAGAGAGC
    ACAGAACGCA GCTATTATCC GGCACAACCC CGATTTGAGG AACCTCCTCC GATGAGCTAC
    GATGGCAGAC CTCGCTATGA GCATGCACCC AAAAACTTCA GCTTGCCACA AGTGCGGTAT
    GAAGATCAAC ATCCTGTTGG CTATGACACA CATGCGCATG GTAGATATAA ACAAGAAGCT
    CAGCCATACC AGTCAGCCAT ATCTCGATCT CCTGAACCCA AGCAGTACTT TGATCCACAT
    GTGAGGGGCT ATGAGCAAGG TCCTCCTCAA GCTTACAATG CAAAAGCTGG ACAGTTTGAG
    CCTTCTCATA GCACTTCAGG TGTTTCTCTT CCTCCTCCCG CTTCTTCACA AACAAAACCA
    GAAGTTTTGC CTTCCAACAG TAAACCACTG CCTACACCAC CATCTCTAGC AGAAGAAGAG
    GAGGATCCAG CCATGAAACC ACAGTCTGTG CGAAGTAGAG TTAAGATATT TGAACGAAGA
    AGATCGCCAT CTCTGGAAAA AATGAAGGAC CCCAGTGACA CGTCAGTTGT CAAACCTCCA
    GAGCTAGCAC CGAAGCCTAC GCTCCCAGCC ATGAGTGGTC CAAAACCAAC TTCTCAAAGC
    CAGTATGAAC ATGATAAAAC AACTTACAGG GCCCCAGAAC CACAGAGACC TCAAGTGAAG
    CCACCTGAAG ATATCGTGCG TTCAAATCAT TATGATCCCG AAGAAGATGA AGAGTATTAC
    AGAAAGCAGC TCTCATACTT TGATCGCAGG AATTTTGAAA ATAAGCCTTC TGCACAGGTT
    CCTGCCAGCC ATCATTCTGA CTCCACTAAA CCAATACATT CACAAAATCA GCTGAATTTC
    TCCAATTATT CTAAGGGGAA GCCAACTGAT GCTGAACCAA TGGATAGATC TGTTGGTGAC
    AAGCGCTATG AGCCAATCCC TCAAGTTACA ACTCCTCCTC CTGCTCCTTC AGTACAGTAT
    ACACAACCTC AGTCAATTAA TAGTCCTGTC CTGTCTCTCC CAGCGCATCA CAAGCCTGCA
    CTTTCTGAAG TTAACTCTGT GTCTGACCCT CCTCCACCTC AGAATAAGCC AGCAATTTTC
    AGATCCTCTA GAGAGGACAC TGTGCAGTCT ACTTTTTATC CTCAGAAAAG CTTCCCTGAC
    AAAGGTCCTG TTAATGGAAC TGAACAGGTT CAGAAAACAG TCACTCCTTC TTACAACCGT
    TTTACAACAA AACCTTACAC TAGTGCTGCG AGGCCCTTTG AGCGCAAGTT TGAAAGTCCT
    AAATTCAACC ATAATCTCTT GCCAAATGAA GCTCAACATA AACCAGAGTT GCCATCAAAA
    TCTCCAAATT CTCCTCAACC AATTTTGAAA GCACACAGCT CATCACAGCC TCCTGAGTTT
    GACAGTGGAA TGGACACCTT CACTGTACAG GTTGACAAGC CTAAATATCA ACCAAATAAT
    GTAAATGCTG TGCCTAAAGC CATTCCTGTA AGCCCTTCAG CACTAGAGGA TGAGGAGGAA
    GAAGACGGAC ACACTGTTGT AGCCACAGCA AGAGGTGTAT TCAACAGCAA TGGTGGCGTA
    TTGAGCTCCA TAGAGACTGG AGTTAGTATT ATTATACCAC AAGGAGCCAT TCCAGAGGGA
    ATAGAGCAAG AAATATATTT TAAAGTCTGC AGAGACAACA GTATACTTCC ACCTTTGGAC
    AAAGAGAAAG GTGAAACACT TCTTAGCCCC TTGGTAATGT GTGGGCCTCA CGGACTAAAA
    TTCCTGAAGC CAGTGGAGCT GCGCCTGCCA CACTGTGACC CCAAAACCTG GCAGAACAAG
    TCTCTTCCTG GAGATCCAAA CTATCTTGTT GGAGCAAACT GTGTCTCAGT CCTAATTGAC
    CACTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa143495
    Clone ID Related Accession (Same CDS sequence) XM_015278979.2
    Accession Version XM_015278979.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5337bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product tight junction protein ZO-1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006097.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015278979.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGCAGCGGC TGGAGAGACT CTGTCGGTGG CGGCGGCGGG GACGCGGCAG GGACAGGGGG 
    GGCGACGGCA AAATGAAGTA TCAGAAATAC CTGACTGTCT TGCAGATGGC CATAGGGGTG
    ACCGCCTCCA ACAGGGGCAG TCTGATGCCC TTAAAGAGGA AGCTGTGGGT AACTCCATCT
    TCCGAAAATC CCAATGGTGC TACCTGTAGT GTAAGTCAGG GAAAGCCTTC TTTAAGAAGA
    ATAAAAGGGA GAATACACAG AAGCAAAAGC CTTGATAGCA TTGATTTCTG TGAATTCACT
    AGTGCAACAA TGGAAGAAAC AGCTATATGG GAACAACACA CGGTGACTCT TCACAGGGCT
    CCTGGATTTG GGTTTGGTAT TGCAATATCT GGAGGAAGGG ATAATCCTCA CTTTCAAAGT
    GGTGAGACCT CAATAGTTAT TTCTGATGTG CTGAAAGGAG GCCCAGCAGA AGGACTGCTA
    CAAGAAAATG ACCGTGTTGC AATGGTTAAT GGAGTTTCAA TGGATAATGT TGAGCACGCA
    TTTGCTGTTC AGCAGCTGAG GAAAAGTGGG AAGAATGCCA AAATTACTAT CCGAAGGATG
    AAGAAAATTC AGATTCCAGT AGCTCGACAG GAACCTGAGC CAGTGTCTGA AAATGAGGAC
    GATAGCTATG ATGAAGAGAT ACGGGATCCA AGGACCAGCC GTGGTGCTTC CAGTGCCAAC
    AGAAGGCATG AGAAGAGCTG GGTACGAGAC CGAAGTGCAA GCAGAGAAAG AAGCTTATCA
    CCAAGATCTG ACAGAAGATC GGTTACTTCC AGTCAGCCTG CAAAACCTAC CAAAGTAACG
    TTGGTGAAAT CAAGGAAAAA TGAAGAGTAT GGTCTACGGT TGGCAAGCCA CATATTTGTG
    AAAGAAATAT CACAGGATAG CCTTGCAGCA AGAGATGGCA ATATTCAGGA AGGGGATGTT
    GTTCTGAAGA TAAATGGCAC AGTGACAGAA AATATGTCCT TAGCAGATGC AAAAACGCTA
    ATAGAAAGGT CCAAAGGCAA GTTGAAGATG GTGGTTCAGC GAGATGAACG AGCTACACTG
    TTGAATGTCC CTGATCTTTC TGACAGTATT CATTCTGCTA ATGCTTCGGA AAGAGATGAC
    ATCTCAGAAA TTCAGTCGCT GGCATCAGAT CATTCCAACC GATCACATGA CAGGCCCCGC
    CGCAGTCGTT CACGATCTCC TGACCAGAGG TCAGAGCCTT CAGACCATTC CAGACATTCT
    CCACAGCAGC CCAGCAACGG CAGTCTTCGA AGCAGAGAAG ATGAAAGGGT AACTAAACCA
    GGAGCCATCT CTACACCTGT AAAGAATGCA GATGATATTT CTAAAACTAT GGAAGAGGTG
    TCAGTCGAAA GAACTGAAAA ACAAACACCA CCGCTCCCAG AACCAAAGCC AGTATATGCA
    CAAGGAGGTC AGCCAGATGT GGATTTACCA GTCAGTCCGT CTGATGGTCC TTTACCCAAT
    TCAACCCATG AAGATGGAAT GCTTCGGCCA AGCATGAAAC TGGTAAAATT CAGAAAAGGA
    GATAGTGTGG GCCTGCGGCT CGCTGGTGGC AATGATGTTG GCATATTTGT AGCTGGTGTT
    CTAGAGGACA GCCCTGCAGC GAAAGAAGGA TTAGAGGAAG GAGACCAAAT TCTCAGGGTT
    AATAACGTAG ACTTCACAAA TATCATCAGA GAAGAAGCTG TCCTTTTTTT GCTTGATCTC
    CCTAAAGGCG AAGAAGTAAC CATTTTGGCA CAGAAGAAGA AAGATGTTTA TCGTCGCATT
    GTTGAGTCTG ATGTAGGGGA TTCTTTCTAT ATCAGAACTC ATTTTGAATA TGAAAAGGAA
    TCTCCTTATG GACTAAGCTT CAACAAAGGT GAAGTGTTTC GGGTTGTGGA CACGCTATAT
    AATGGCAAAC TGGGTTCGTG GCTGGCAATT CGAATTGGCA AGAATCATAA GGAAGTTGAA
    AGAGGTATCA TACCTAACAA AAACAGAGCT GAACAACTAG CTAGTGTACA GTACACGCTT
    CCAAAGACAG CAGGAGGAGA TCGAGCGGAT TTCTGGAGAT TCCGAGGTTT GCGTAGCTCA
    AAAAGAAATC TCCGAAAAAG TAGAGAAGAT CTTTCTGCTC AGCCTGTTCA GACAAAGTTC
    CCTGCCTATG AAAGAGTTGT TCTTCGGGAA GCTGGGTTTC TCAGACCTGT GACCATTTTT
    GGTCCAATAG CTGATGTTGC ACGAGAGAAA CTAGCCAGAG AAGAACCAGA TATCTTTCAA
    ATTGCAAAAA GTGAACCGAG AGATGCTGGT ACTGACCAAC GTAGTTCTGG CATTATTCGT
    CTTCATACGA TAAAACAGAT AATTGATAGA GACAAACACG CTTTATTAGA TGTCACTCCT
    AATGCAGTGG ACCGTCTGAA TTATGCACAG TGGTATCCTA TTGTAGTGTT TCTGAACCCT
    GATTCTAAGC AGGGTGTGAA GACCATGAGA ATGAGGCTGT GCCCAGAATC ACGAAAAAGT
    GCCAGGAAGC TATATGAAAG AGCTCACAAG CTACGCAAAA ATAATCACCA CCTCTTTACA
    ACTACCATTA ACTTGAATTC AATGAATGAA GGATGGTATG GAGCTCTAAA AGAAGCAATT
    CAGCAACAAC AAAACCAACT GGTGTGGGTT TCTGAAGGAA AGGCAGATGG TGCTACAAGT
    GATGACCTTG ATTTACATGA TGACCGCCTG TCTTACCTCT CAGCTCCTGG TAGTGAATAT
    TCTATGTACA GTACAGACAG CAGACATACA TCTGACTATG AAGACACAGA TACAGAAGGT
    GGTGCTTATA CTGATCAAGA ACTGGATGAA ACTCTGAACG ATGAGGTTGG AACTCCTCCT
    GAATCTGCTA TTACACGTTC TTCTGAACCC GTTAGGGAGG ATTCTTCTGG AATGCATCAT
    GAAACTCAAA CTTACACTAG TTACGCATCT CAAGCTCAGC CTCAGCCAAA TCTTAGAATA
    GATTCTTCAG GATTTAAAAC AACTGCTTCT CAGCCGGTAT ACAGAAAGGA TCCATATATT
    AGTGAAGAAG CATCCAGACA AAGCTATGTC TTAAAACAGC CAGCTGTTAA TCACCCAGTA
    CAAAGACAGG AAAGAGACCC AAATCTGATC TATGAATCTC AGACTCAGTA TGCAGAAAAA
    CAACCAAGTA GGGAGTACGA GCAGTCAACA TACAGGTATG ATTCTACAAA CTATGTAGAT
    CAATTTTCTC GTGGGTATGA CCCTCGTCTA CATTATGAAG ATCGTGCGCC TCCCTATGAG
    GAGCACTGGT CATATTATGA TGAAAAACAG CCCTACAATC AACCAAGAAC AGCTTATGAA
    AGCCAGCCTC CTAGAGATCT TGATTCCAGA CAAAATACAG AAGAGAGCAC AGAACGCAGC
    TATTATCCGG CACAACCCCG ATTTGAGGAA CCTCCTCCGA TGAGCTACGA TGGCAGACCT
    CGCTATGAGC ATGCACCCAA AAACTTCAGC TTGCCACAAG TGCGGTATGA AGATCAACAT
    CCTGTTGGCT ATGACACACA TGCGCATGGT AGATATAAAC AAGAAGCTCA GCCATACCAG
    TCAGCCATAT CTCGATCTCC TGAACCCAAG CAGTACTTTG ATCCACATGT GAGGGGCTAT
    GAGCAAGGTC CTCCTCAAGC TTACAATGCA AAAGCTGGAC AGTTTGAGCC TTCTCATAGC
    ACTTCAGGTG TTTCTCTTCC TCCTCCCGCT TCTTCACAAA CAAAACCAGA AGTTTTGCCT
    TCCAACAGTA AACCACTGCC TACACCACCA TCTCTAGCAG AAGAAGAGGA GGATCCAGCC
    ATGAAACCAC AGTCTGTGCG AAGTAGAGTT AAGATATTTG AACGAAGAAG ATCGCCATCT
    CTGGAAAAAA TGAAGGACCC CAGTGACACG TCAGTTGTCA AACCTCCAGA GCTAGCACCG
    AAGCCTACGC TCCCAGCCAT GAGTGGTCCA AAACCAACTT CTCAAAGCCA GTATGAACAT
    GATAAAACAA CTTACAGGGC CCCAGAACCA CAGAGACCTC AAGTGAAGCC ACCTGAAGAT
    ATCGTGCGTT CAAATCATTA TGATCCCGAA GAAGATGAAG AGTATTACAG AAAGCAGCTC
    TCATACTTTG ATCGCAGGAA TTTTGAAAAT AAGCCTTCTG CACAGGTTCC TGCCAGCCAT
    CATTCTGACT CCACTAAACC AATACATTCA CAAAATCAGC TGAATTTCTC CAATTATTCT
    AAGGGGAAGC CAACTGATGC TGAACCAATG GATAGATCTG TTGGTGACAA GCGCTATGAG
    CCAATCCCTC AAGTTACAAC TCCTCCTCCT GCTCCTTCAG TACAGTATAC ACAACCTCAG
    TCAATTAATA GTCCTGTCCT GTCTCTCCCA GCGCATCACA AGCCTGCACT TTCTGAAGTT
    AACTCTGTGT CTGACCCTCC TCCACCTCAG AATAAGCCAG CAATTTTCAG ATCCTCTAGA
    GAGGACACTG TGCAGTCTAC TTTTTATCCT CAGAAAAGCT TCCCTGACAA AGGTCCTGTT
    AATGGAACTG AACAGGTTCA GAAAACAGTC ACTCCTTCTT ACAACCGTTT TACAACAAAA
    CCTTACACTA GTGCTGCGAG GCCCTTTGAG CGCAAGTTTG AAAGTCCTAA ATTCAACCAT
    AATCTCTTGC CAAATGAAGC TCAACATAAA CCAGAGTTGC CATCAAAATC TCCAAATTCT
    CCTCAACCAA TTTTGAAAGC ACACAGCTCA TCACAGCCTC CTGAGTTTGA CAGTGGAATG
    GACACCTTCA CTGTACAGGT TGACAAGCCT AAATATCAAC CAAATAATGT AAATGCTGTG
    CCTAAAGCCA TTCCTGTAAG CCCTTCAGCA CTAGAGGATG AGGAGGAAGA AGACGGACAC
    ACTGTTGTAG CCACAGCAAG AGGTGTATTC AACAGCAATG GTGGCGTATT GAGCTCCATA
    GAGACTGGAG TTAGTATTAT TATACCACAA GGAGCCATTC CAGAGGGAAT AGAGCAAGAA
    ATATATTTTA AAGTCTGCAG AGACAACAGT ATACTTCCAC CTTTGGACAA AGAGAAAGGT
    GAAACACTTC TTAGCCCCTT GGTAATGTGT GGGCCTCACG GACTAAAATT CCTGAAGCCA
    GTGGAGCTGC GCCTGCCACA CTGTGCGTCT ATGACCCCTG ATGGTTGGTC TTTTGCTCTA
    AAATCCTCTG ACTCCTCGTC GGGTGACCCC AAAACCTGGC AGAACAAGTC TCTTCCTGGA
    GATCCAAACT ATCTTGTTGG AGCAAACTGT GTCTCAGTCC TAATTGACCA CTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015134465.2
    CDS16..5352
    Translation

    Target ORF information:

    RefSeq Version XM_015278979.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus tight junction protein 1 (TJP1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015278979.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGCAGCGGC TGGAGAGACT CTGTCGGTGG CGGCGGCGGG GACGCGGCAG GGACAGGGGG 
    GGCGACGGCA AAATGAAGTA TCAGAAATAC CTGACTGTCT TGCAGATGGC CATAGGGGTG
    ACCGCCTCCA ACAGGGGCAG TCTGATGCCC TTAAAGAGGA AGCTGTGGGT AACTCCATCT
    TCCGAAAATC CCAATGGTGC TACCTGTAGT GTAAGTCAGG GAAAGCCTTC TTTAAGAAGA
    ATAAAAGGGA GAATACACAG AAGCAAAAGC CTTGATAGCA TTGATTTCTG TGAATTCACT
    AGTGCAACAA TGGAAGAAAC AGCTATATGG GAACAACACA CGGTGACTCT TCACAGGGCT
    CCTGGATTTG GGTTTGGTAT TGCAATATCT GGAGGAAGGG ATAATCCTCA CTTTCAAAGT
    GGTGAGACCT CAATAGTTAT TTCTGATGTG CTGAAAGGAG GCCCAGCAGA AGGACTGCTA
    CAAGAAAATG ACCGTGTTGC AATGGTTAAT GGAGTTTCAA TGGATAATGT TGAGCACGCA
    TTTGCTGTTC AGCAGCTGAG GAAAAGTGGG AAGAATGCCA AAATTACTAT CCGAAGGATG
    AAGAAAATTC AGATTCCAGT AGCTCGACAG GAACCTGAGC CAGTGTCTGA AAATGAGGAC
    GATAGCTATG ATGAAGAGAT ACGGGATCCA AGGACCAGCC GTGGTGCTTC CAGTGCCAAC
    AGAAGGCATG AGAAGAGCTG GGTACGAGAC CGAAGTGCAA GCAGAGAAAG AAGCTTATCA
    CCAAGATCTG ACAGAAGATC GGTTACTTCC AGTCAGCCTG CAAAACCTAC CAAAGTAACG
    TTGGTGAAAT CAAGGAAAAA TGAAGAGTAT GGTCTACGGT TGGCAAGCCA CATATTTGTG
    AAAGAAATAT CACAGGATAG CCTTGCAGCA AGAGATGGCA ATATTCAGGA AGGGGATGTT
    GTTCTGAAGA TAAATGGCAC AGTGACAGAA AATATGTCCT TAGCAGATGC AAAAACGCTA
    ATAGAAAGGT CCAAAGGCAA GTTGAAGATG GTGGTTCAGC GAGATGAACG AGCTACACTG
    TTGAATGTCC CTGATCTTTC TGACAGTATT CATTCTGCTA ATGCTTCGGA AAGAGATGAC
    ATCTCAGAAA TTCAGTCGCT GGCATCAGAT CATTCCAACC GATCACATGA CAGGCCCCGC
    CGCAGTCGTT CACGATCTCC TGACCAGAGG TCAGAGCCTT CAGACCATTC CAGACATTCT
    CCACAGCAGC CCAGCAACGG CAGTCTTCGA AGCAGAGAAG ATGAAAGGGT AACTAAACCA
    GGAGCCATCT CTACACCTGT AAAGAATGCA GATGATATTT CTAAAACTAT GGAAGAGGTG
    TCAGTCGAAA GAACTGAAAA ACAAACACCA CCGCTCCCAG AACCAAAGCC AGTATATGCA
    CAAGGAGGTC AGCCAGATGT GGATTTACCA GTCAGTCCGT CTGATGGTCC TTTACCCAAT
    TCAACCCATG AAGATGGAAT GCTTCGGCCA AGCATGAAAC TGGTAAAATT CAGAAAAGGA
    GATAGTGTGG GCCTGCGGCT CGCTGGTGGC AATGATGTTG GCATATTTGT AGCTGGTGTT
    CTAGAGGACA GCCCTGCAGC GAAAGAAGGA TTAGAGGAAG GAGACCAAAT TCTCAGGGTT
    AATAACGTAG ACTTCACAAA TATCATCAGA GAAGAAGCTG TCCTTTTTTT GCTTGATCTC
    CCTAAAGGCG AAGAAGTAAC CATTTTGGCA CAGAAGAAGA AAGATGTTTA TCGTCGCATT
    GTTGAGTCTG ATGTAGGGGA TTCTTTCTAT ATCAGAACTC ATTTTGAATA TGAAAAGGAA
    TCTCCTTATG GACTAAGCTT CAACAAAGGT GAAGTGTTTC GGGTTGTGGA CACGCTATAT
    AATGGCAAAC TGGGTTCGTG GCTGGCAATT CGAATTGGCA AGAATCATAA GGAAGTTGAA
    AGAGGTATCA TACCTAACAA AAACAGAGCT GAACAACTAG CTAGTGTACA GTACACGCTT
    CCAAAGACAG CAGGAGGAGA TCGAGCGGAT TTCTGGAGAT TCCGAGGTTT GCGTAGCTCA
    AAAAGAAATC TCCGAAAAAG TAGAGAAGAT CTTTCTGCTC AGCCTGTTCA GACAAAGTTC
    CCTGCCTATG AAAGAGTTGT TCTTCGGGAA GCTGGGTTTC TCAGACCTGT GACCATTTTT
    GGTCCAATAG CTGATGTTGC ACGAGAGAAA CTAGCCAGAG AAGAACCAGA TATCTTTCAA
    ATTGCAAAAA GTGAACCGAG AGATGCTGGT ACTGACCAAC GTAGTTCTGG CATTATTCGT
    CTTCATACGA TAAAACAGAT AATTGATAGA GACAAACACG CTTTATTAGA TGTCACTCCT
    AATGCAGTGG ACCGTCTGAA TTATGCACAG TGGTATCCTA TTGTAGTGTT TCTGAACCCT
    GATTCTAAGC AGGGTGTGAA GACCATGAGA ATGAGGCTGT GCCCAGAATC ACGAAAAAGT
    GCCAGGAAGC TATATGAAAG AGCTCACAAG CTACGCAAAA ATAATCACCA CCTCTTTACA
    ACTACCATTA ACTTGAATTC AATGAATGAA GGATGGTATG GAGCTCTAAA AGAAGCAATT
    CAGCAACAAC AAAACCAACT GGTGTGGGTT TCTGAAGGAA AGGCAGATGG TGCTACAAGT
    GATGACCTTG ATTTACATGA TGACCGCCTG TCTTACCTCT CAGCTCCTGG TAGTGAATAT
    TCTATGTACA GTACAGACAG CAGACATACA TCTGACTATG AAGACACAGA TACAGAAGGT
    GGTGCTTATA CTGATCAAGA ACTGGATGAA ACTCTGAACG ATGAGGTTGG AACTCCTCCT
    GAATCTGCTA TTACACGTTC TTCTGAACCC GTTAGGGAGG ATTCTTCTGG AATGCATCAT
    GAAACTCAAA CTTACACTAG TTACGCATCT CAAGCTCAGC CTCAGCCAAA TCTTAGAATA
    GATTCTTCAG GATTTAAAAC AACTGCTTCT CAGCCGGTAT ACAGAAAGGA TCCATATATT
    AGTGAAGAAG CATCCAGACA AAGCTATGTC TTAAAACAGC CAGCTGTTAA TCACCCAGTA
    CAAAGACAGG AAAGAGACCC AAATCTGATC TATGAATCTC AGACTCAGTA TGCAGAAAAA
    CAACCAAGTA GGGAGTACGA GCAGTCAACA TACAGGTATG ATTCTACAAA CTATGTAGAT
    CAATTTTCTC GTGGGTATGA CCCTCGTCTA CATTATGAAG ATCGTGCGCC TCCCTATGAG
    GAGCACTGGT CATATTATGA TGAAAAACAG CCCTACAATC AACCAAGAAC AGCTTATGAA
    AGCCAGCCTC CTAGAGATCT TGATTCCAGA CAAAATACAG AAGAGAGCAC AGAACGCAGC
    TATTATCCGG CACAACCCCG ATTTGAGGAA CCTCCTCCGA TGAGCTACGA TGGCAGACCT
    CGCTATGAGC ATGCACCCAA AAACTTCAGC TTGCCACAAG TGCGGTATGA AGATCAACAT
    CCTGTTGGCT ATGACACACA TGCGCATGGT AGATATAAAC AAGAAGCTCA GCCATACCAG
    TCAGCCATAT CTCGATCTCC TGAACCCAAG CAGTACTTTG ATCCACATGT GAGGGGCTAT
    GAGCAAGGTC CTCCTCAAGC TTACAATGCA AAAGCTGGAC AGTTTGAGCC TTCTCATAGC
    ACTTCAGGTG TTTCTCTTCC TCCTCCCGCT TCTTCACAAA CAAAACCAGA AGTTTTGCCT
    TCCAACAGTA AACCACTGCC TACACCACCA TCTCTAGCAG AAGAAGAGGA GGATCCAGCC
    ATGAAACCAC AGTCTGTGCG AAGTAGAGTT AAGATATTTG AACGAAGAAG ATCGCCATCT
    CTGGAAAAAA TGAAGGACCC CAGTGACACG TCAGTTGTCA AACCTCCAGA GCTAGCACCG
    AAGCCTACGC TCCCAGCCAT GAGTGGTCCA AAACCAACTT CTCAAAGCCA GTATGAACAT
    GATAAAACAA CTTACAGGGC CCCAGAACCA CAGAGACCTC AAGTGAAGCC ACCTGAAGAT
    ATCGTGCGTT CAAATCATTA TGATCCCGAA GAAGATGAAG AGTATTACAG AAAGCAGCTC
    TCATACTTTG ATCGCAGGAA TTTTGAAAAT AAGCCTTCTG CACAGGTTCC TGCCAGCCAT
    CATTCTGACT CCACTAAACC AATACATTCA CAAAATCAGC TGAATTTCTC CAATTATTCT
    AAGGGGAAGC CAACTGATGC TGAACCAATG GATAGATCTG TTGGTGACAA GCGCTATGAG
    CCAATCCCTC AAGTTACAAC TCCTCCTCCT GCTCCTTCAG TACAGTATAC ACAACCTCAG
    TCAATTAATA GTCCTGTCCT GTCTCTCCCA GCGCATCACA AGCCTGCACT TTCTGAAGTT
    AACTCTGTGT CTGACCCTCC TCCACCTCAG AATAAGCCAG CAATTTTCAG ATCCTCTAGA
    GAGGACACTG TGCAGTCTAC TTTTTATCCT CAGAAAAGCT TCCCTGACAA AGGTCCTGTT
    AATGGAACTG AACAGGTTCA GAAAACAGTC ACTCCTTCTT ACAACCGTTT TACAACAAAA
    CCTTACACTA GTGCTGCGAG GCCCTTTGAG CGCAAGTTTG AAAGTCCTAA ATTCAACCAT
    AATCTCTTGC CAAATGAAGC TCAACATAAA CCAGAGTTGC CATCAAAATC TCCAAATTCT
    CCTCAACCAA TTTTGAAAGC ACACAGCTCA TCACAGCCTC CTGAGTTTGA CAGTGGAATG
    GACACCTTCA CTGTACAGGT TGACAAGCCT AAATATCAAC CAAATAATGT AAATGCTGTG
    CCTAAAGCCA TTCCTGTAAG CCCTTCAGCA CTAGAGGATG AGGAGGAAGA AGACGGACAC
    ACTGTTGTAG CCACAGCAAG AGGTGTATTC AACAGCAATG GTGGCGTATT GAGCTCCATA
    GAGACTGGAG TTAGTATTAT TATACCACAA GGAGCCATTC CAGAGGGAAT AGAGCAAGAA
    ATATATTTTA AAGTCTGCAG AGACAACAGT ATACTTCCAC CTTTGGACAA AGAGAAAGGT
    GAAACACTTC TTAGCCCCTT GGTAATGTGT GGGCCTCACG GACTAAAATT CCTGAAGCCA
    GTGGAGCTGC GCCTGCCACA CTGTGCGTCT ATGACCCCTG ATGGTTGGTC TTTTGCTCTA
    AAATCCTCTG ACTCCTCGTC GGGTGACCCC AAAACCTGGC AGAACAAGTC TCTTCCTGGA
    GATCCAAACT ATCTTGTTGG AGCAAACTGT GTCTCAGTCC TAATTGACCA CTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa143496
    Clone ID Related Accession (Same CDS sequence) XM_015278981.2
    Accession Version XM_015278981.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5277bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product tight junction protein ZO-1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006097.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015278981.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGCAGCGGC TGGAGAGACT CTGTCGGTGG CGGCGGCGGG GACGCGGCAG GGACAGGGGG 
    GGCGACGGCA AAATGAAGTA TCAGAAATAC CTGACTGTCT TGCAGATGGC CATAGGGGTG
    ACCGCCTCCA ACAGGGGCAG TCTGATGCCC TTAAAGAGGA AGCTGTGGGT AACTCCATCT
    TCCGAAAATC CCAATGGTGC TACCTGTAGT GTAAGTCAGG GAAAGCCTTC TTTAAGAAGA
    ATAAAAGGGA GAATACACAG AAGCAAAAGC CTTGATAGCA TTGATTTCTG TGAATTCACT
    AGTGCAACAA TGGAAGAAAC AGCTATATGG GAACAACACA CGGTGACTCT TCACAGGGCT
    CCTGGATTTG GGTTTGGTAT TGCAATATCT GGAGGAAGGG ATAATCCTCA CTTTCAAAGT
    GGTGAGACCT CAATAGTTAT TTCTGATGTG CTGAAAGGAG GCCCAGCAGA AGGACTGCTA
    CAAGAAAATG ACCGTGTTGC AATGGTTAAT GGAGTTTCAA TGGATAATGT TGAGCACGCA
    TTTGCTGTTC AGCAGCTGAG GAAAAGTGGG AAGAATGCCA AAATTACTAT CCGAAGGATG
    AAGAAAATTC AGATTCCAGT AGCTCGACAG GAACCTGAGC CAGTGTCTGA AAATGAGGAC
    GATAGCTATG ATGAAGAGAT ACGGGATCCA AGGACCAGCC GTGGTGCTTC CAGTGCCAAC
    AGAAGGCATG AGAAGAGCTG GGTACGAGAC CGAAGTGCAA GCAGAGAAAG AAGCTTATCA
    CCAAGATCTG ACAGAAGATC GGTTACTTCC AGTCAGCCTG CAAAACCTAC CAAAGTAACG
    TTGGTGAAAT CAAGGAAAAA TGAAGAGTAT GGTCTACGGT TGGCAAGCCA CATATTTGTG
    AAAGAAATAT CACAGGATAG CCTTGCAGCA AGAGATGGCA ATATTCAGGA AGGGGATGTT
    GTTCTGAAGA TAAATGGCAC AGTGACAGAA AATATGTCCT TAGCAGATGC AAAAACGCTA
    ATAGAAAGGT CCAAAGGCAA GTTGAAGATG GTGGTTCAGC GAGATGAACG AGCTACACTG
    TTGAATGTCC CTGATCTTTC TGACAGTATT CATTCTGCTA ATGCTTCGGA AAGAGATGAC
    ATCTCAGAAA TTCAGTCGCT GGCATCAGAT CATTCCAACC GATCACATGA CAGGCCCCGC
    CGCAGTCGTT CACGATCTCC TGACCAGAGG TCAGAGCCTT CAGACCATTC CAGACATTCT
    CCACAGCAGC CCAGCAACGG CAGTCTTCGA AGCAGAGAAG ATGAAAGGGT AACTAAACCA
    GGAGCCATCT CTACACCTGT AAAGAATGCA GATGATATTT CTAAAACTAT GGAAGAGGTG
    TCAGTCGAAA GAACTGAAAA ACAAACACCA CCGCTCCCAG AACCAAAGCC AGTATATGCA
    CAAGGAGGTC AGCCAGATGT GGATTTACCA GTCAGTCCGT CTGATGGTCC TTTACCCAAT
    TCAACCCATG AAGATGGAAT GCTTCGGCCA AGCATGAAAC TGGTAAAATT CAGAAAAGGA
    GATAGTGTGG GCCTGCGGCT CGCTGGTGGC AATGATGTTG GCATATTTGT AGCTGGTGTT
    CTAGAGGACA GCCCTGCAGC GAAAGAAGGA TTAGAGGAAG GAGACCAAAT TCTCAGGGTT
    AATAACGTAG ACTTCACAAA TATCATCAGA GAAGAAGCTG TCCTTTTTTT GCTTGATCTC
    CCTAAAGGCG AAGAAGTAAC CATTTTGGCA CAGAAGAAGA AAGATGTTTA TCGTCGCATT
    GTTGAGTCTG ATGTAGGGGA TTCTTTCTAT ATCAGAACTC ATTTTGAATA TGAAAAGGAA
    TCTCCTTATG GACTAAGCTT CAACAAAGGT GAAGTGTTTC GGGTTGTGGA CACGCTATAT
    AATGGCAAAC TGGGTTCGTG GCTGGCAATT CGAATTGGCA AGAATCATAA GGAAGTTGAA
    AGAGGTATCA TACCTAACAA AAACAGAGCT GAACAACTAG CTAGTGTACA GTACACGCTT
    CCAAAGACAG CAGGAGGAGA TCGAGCGGAT TTCTGGAGAT TCCGAGGTTT GCGTAGCTCA
    AAAAGAAATC TCCGAAAAAG TAGAGAAGAT CTTTCTGCTC AGCCTGTTCA GACAAAGTTC
    CCTGCCTATG AAAGAGTTGT TCTTCGGGAA GCTGGGTTTC TCAGACCTGT GACCATTTTT
    GGTCCAATAG CTGATGTTGC ACGAGAGAAA CTAGCCAGAG AAGAACCAGA TATCTTTCAA
    ATTGCAAAAA GTGAACCGAG AGATGCTGGT ACTGACCAAC GTAGTTCTGG CATTATTCGT
    CTTCATACGA TAAAACAGAT AATTGATAGA GACAAACACG CTTTATTAGA TGTCACTCCT
    AATGCAGTGG ACCGTCTGAA TTATGCACAG TGGTATCCTA TTGTAGTGTT TCTGAACCCT
    GATTCTAAGC AGGGTGTGAA GACCATGAGA ATGAGGCTGT GCCCAGAATC ACGAAAAAGT
    GCCAGGAAGC TATATGAAAG AGCTCACAAG CTACGCAAAA ATAATCACCA CCTCTTTACA
    ACTACCATTA ACTTGAATTC AATGAATGAA GGATGGTATG GAGCTCTAAA AGAAGCAATT
    CAGCAACAAC AAAACCAACT GGTGTGGGTT TCTGAAGGAA AGGCAGATGG TGCTACAAGT
    GATGACCTTG ATTTACATGA TGACCGCCTG TCTTACCTCT CAGCTCCTGG TAGTGAATAT
    TCTATGTACA GTACAGACAG CAGACATACA TCTGACTATG AAGACACAGA TACAGAAGGT
    GGTGCTTATA CTGATCAAGA ACTGGATGAA ACTCTGAACG ATGAGGTTGG AACTCCTCCT
    GAATCTGCTA TTACACGTTC TTCTGAACCC GTTAGGGAGG ATTCTTCTGG AATGCATCAT
    GAAACTCAAA CTTACACTAG TTACGCATCT CAAGCTCAGC CTCAGCCAAA TCTTAGAATA
    GATTCTTCAG GATTTAAAAC AACTGCTTCT CAGCCGGTAT ACAGAAAGGA TCCATATATT
    AGTGAAGAAG CATCCAGACA AAGCTATGTC TTAAAACAGC CAGCTGTTAA TCACCCAGTA
    CAAAGACAGG AAAGAGACCC AAATCTGATC TATGAATCTC AGACTCAGTA TGCAGAAAAA
    CAACCAAGTA GGGAGTACGA GCAGTCAACA TACAGGTATG ATTCTACAAA CTATGTAGAT
    CAATTTTCTC GTGGGTATGA CCCTCGTCTA CATTATGAAG ATCGTGCGCC TCCCTATGAG
    GAGCACTGGT CATATTATGA TGAAAAACAG CCCTACAATC AACCAAGAAC AGCTTATGAA
    AGCCAGCCTC CTAGAGATCT TGATTCCAGA CAAAATACAG AAGAGAGCAC AGAACGCAGC
    TATTATCCGG CACAACCCCG ATTTGAGGAA CCTCCTCCGA TGAGCTACGA TGGCAGACCT
    CGCTATGAGC ATGCACCCAA AAACTTCAGC TTGCCACAAG TGCGGTATGA AGATCAACAT
    CCTGTTGGCT ATGACACACA TGCGCATGGT AGATATAAAC AAGAAGCTCA GCCATACCAG
    TCAGCCATAT CTCGATCTCC TGAACCCAAG CAGTACTTTG ATCCACATGT GAGGGGCTAT
    GAGCAAGGTC CTCCTCAAGC TTACAATGCA AAAGCTGGAC AGTTTGAGCC TTCTCATAGC
    ACTTCAGGTG TTTCTCTTCC TCCTCCCGCT TCTTCACAAA CAAAACCAGA AGTTTTGCCT
    TCCAACAGTA AACCACTGCC TACACCACCA TCTCTAGCAG AAGAAGAGGA GGATCCAGCC
    ATGAAACCAC AGTCTGTGCG AAGTAGAGTT AAGATATTTG AACGAAGAAG ATCGCCATCT
    CTGGAAAAAA TGAAGGACCC CAGTGACACG TCAGTTGTCA AACCTCCAGA GCTAGCACCG
    AAGCCTACGC TCCCAGCCAT GAGTGGTCCA AAACCAACTT CTCAAAGCCA GTATGAACAT
    GATAAAACAA CTTACAGGGC CCCAGAACCA CAGAGACCTC AAGTGAAGCC ACCTGAAGAT
    ATCGTGCGTT CAAATCATTA TGATCCCGAA GAAGATGAAG AGTATTACAG AAAGCAGCTC
    TCATACTTTG ATCGCAGGAA TTTTGAAAAT AAGCCTTCTG CACAGGTTCC TGCCAGCCAT
    CATTCTGACT CCACTAAACC AATACATTCA CAAAATCAGC TGAATTTCTC CAATTATTCT
    AAGGGGAAGC CAACTGATGC TGAACCAATG GATAGATCTG TTGGTGACAA GCGCTATGAG
    CCAATCCCTC AAGTTACAAC TCCTCCTCCT GCTCCTTCAG TACAGTATAC ACAACCTCAG
    TCAATTAATA GTCCTGTCCT GTCTCTCCCA GCGCATCACA AGCCTGCACT TTCTGAAGTT
    AACTCTGTGT CTGACCCTCC TCCACCTCAG AATAAGCCAG CAATTTTCAG ATCCTCTAGA
    GAGGACACTG TGCAGTCTAC TTTTTATCCT CAGAAAAGCT TCCCTGACAA AGGTCCTGTT
    AATGGAACTG AACAGGTTCA GAAAACAGTC ACTCCTTCTT ACAACCGTTT TACAACAAAA
    CCTTACACTA GTGCTGCGAG GCCCTTTGAG CGCAAGTTTG AAAGTCCTAA ATTCAACCAT
    AATCTCTTGC CAAATGAAGC TCAACATAAA CCAGAGTTGC CATCAAAATC TCCAAATTCT
    CCTCAACCAA TTTTGAAAGC ACACAGCTCA TCACAGCCTC CTGAGTTTGA CAGTGGAATG
    GACACCTTCA CTGTACAGGT TGACAAGCCT AAATATCAAC CAAATAATGT AAATGCTGTG
    CCTAAAGCCA TTCCTGTAAG CCCTTCAGCA CTAGAGGATG AGGAGGAAGA AGACGGACAC
    ACTGTTGTAG CCACAGCAAG AGGTGTATTC AACAGCAATG GTGGCGTATT GAGCTCCATA
    GAGACTGGAG TTAGTATTAT TATACCACAA GGAGCCATTC CAGAGGGAAT AGAGCAAGAA
    ATATATTTTA AAGTCTGCAG AGACAACAGT ATACTTCCAC CTTTGGACAA AGAGAAAGGT
    GAAACACTTC TTAGCCCCTT GGTAATGTGT GGGCCTCACG GACTAAAATT CCTGAAGCCA
    GTGGAGCTGC GCCTGCCACA CTGTGACCCC AAAACCTGGC AGAACAAGTC TCTTCCTGGA
    GATCCAAACT ATCTTGTTGG AGCAAACTGT GTCTCAGTCC TAATTGACCA CTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015134467.2
    CDS16..5292
    Translation

    Target ORF information:

    RefSeq Version XM_015278981.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus tight junction protein 1 (TJP1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015278981.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGCAGCGGC TGGAGAGACT CTGTCGGTGG CGGCGGCGGG GACGCGGCAG GGACAGGGGG 
    GGCGACGGCA AAATGAAGTA TCAGAAATAC CTGACTGTCT TGCAGATGGC CATAGGGGTG
    ACCGCCTCCA ACAGGGGCAG TCTGATGCCC TTAAAGAGGA AGCTGTGGGT AACTCCATCT
    TCCGAAAATC CCAATGGTGC TACCTGTAGT GTAAGTCAGG GAAAGCCTTC TTTAAGAAGA
    ATAAAAGGGA GAATACACAG AAGCAAAAGC CTTGATAGCA TTGATTTCTG TGAATTCACT
    AGTGCAACAA TGGAAGAAAC AGCTATATGG GAACAACACA CGGTGACTCT TCACAGGGCT
    CCTGGATTTG GGTTTGGTAT TGCAATATCT GGAGGAAGGG ATAATCCTCA CTTTCAAAGT
    GGTGAGACCT CAATAGTTAT TTCTGATGTG CTGAAAGGAG GCCCAGCAGA AGGACTGCTA
    CAAGAAAATG ACCGTGTTGC AATGGTTAAT GGAGTTTCAA TGGATAATGT TGAGCACGCA
    TTTGCTGTTC AGCAGCTGAG GAAAAGTGGG AAGAATGCCA AAATTACTAT CCGAAGGATG
    AAGAAAATTC AGATTCCAGT AGCTCGACAG GAACCTGAGC CAGTGTCTGA AAATGAGGAC
    GATAGCTATG ATGAAGAGAT ACGGGATCCA AGGACCAGCC GTGGTGCTTC CAGTGCCAAC
    AGAAGGCATG AGAAGAGCTG GGTACGAGAC CGAAGTGCAA GCAGAGAAAG AAGCTTATCA
    CCAAGATCTG ACAGAAGATC GGTTACTTCC AGTCAGCCTG CAAAACCTAC CAAAGTAACG
    TTGGTGAAAT CAAGGAAAAA TGAAGAGTAT GGTCTACGGT TGGCAAGCCA CATATTTGTG
    AAAGAAATAT CACAGGATAG CCTTGCAGCA AGAGATGGCA ATATTCAGGA AGGGGATGTT
    GTTCTGAAGA TAAATGGCAC AGTGACAGAA AATATGTCCT TAGCAGATGC AAAAACGCTA
    ATAGAAAGGT CCAAAGGCAA GTTGAAGATG GTGGTTCAGC GAGATGAACG AGCTACACTG
    TTGAATGTCC CTGATCTTTC TGACAGTATT CATTCTGCTA ATGCTTCGGA AAGAGATGAC
    ATCTCAGAAA TTCAGTCGCT GGCATCAGAT CATTCCAACC GATCACATGA CAGGCCCCGC
    CGCAGTCGTT CACGATCTCC TGACCAGAGG TCAGAGCCTT CAGACCATTC CAGACATTCT
    CCACAGCAGC CCAGCAACGG CAGTCTTCGA AGCAGAGAAG ATGAAAGGGT AACTAAACCA
    GGAGCCATCT CTACACCTGT AAAGAATGCA GATGATATTT CTAAAACTAT GGAAGAGGTG
    TCAGTCGAAA GAACTGAAAA ACAAACACCA CCGCTCCCAG AACCAAAGCC AGTATATGCA
    CAAGGAGGTC AGCCAGATGT GGATTTACCA GTCAGTCCGT CTGATGGTCC TTTACCCAAT
    TCAACCCATG AAGATGGAAT GCTTCGGCCA AGCATGAAAC TGGTAAAATT CAGAAAAGGA
    GATAGTGTGG GCCTGCGGCT CGCTGGTGGC AATGATGTTG GCATATTTGT AGCTGGTGTT
    CTAGAGGACA GCCCTGCAGC GAAAGAAGGA TTAGAGGAAG GAGACCAAAT TCTCAGGGTT
    AATAACGTAG ACTTCACAAA TATCATCAGA GAAGAAGCTG TCCTTTTTTT GCTTGATCTC
    CCTAAAGGCG AAGAAGTAAC CATTTTGGCA CAGAAGAAGA AAGATGTTTA TCGTCGCATT
    GTTGAGTCTG ATGTAGGGGA TTCTTTCTAT ATCAGAACTC ATTTTGAATA TGAAAAGGAA
    TCTCCTTATG GACTAAGCTT CAACAAAGGT GAAGTGTTTC GGGTTGTGGA CACGCTATAT
    AATGGCAAAC TGGGTTCGTG GCTGGCAATT CGAATTGGCA AGAATCATAA GGAAGTTGAA
    AGAGGTATCA TACCTAACAA AAACAGAGCT GAACAACTAG CTAGTGTACA GTACACGCTT
    CCAAAGACAG CAGGAGGAGA TCGAGCGGAT TTCTGGAGAT TCCGAGGTTT GCGTAGCTCA
    AAAAGAAATC TCCGAAAAAG TAGAGAAGAT CTTTCTGCTC AGCCTGTTCA GACAAAGTTC
    CCTGCCTATG AAAGAGTTGT TCTTCGGGAA GCTGGGTTTC TCAGACCTGT GACCATTTTT
    GGTCCAATAG CTGATGTTGC ACGAGAGAAA CTAGCCAGAG AAGAACCAGA TATCTTTCAA
    ATTGCAAAAA GTGAACCGAG AGATGCTGGT ACTGACCAAC GTAGTTCTGG CATTATTCGT
    CTTCATACGA TAAAACAGAT AATTGATAGA GACAAACACG CTTTATTAGA TGTCACTCCT
    AATGCAGTGG ACCGTCTGAA TTATGCACAG TGGTATCCTA TTGTAGTGTT TCTGAACCCT
    GATTCTAAGC AGGGTGTGAA GACCATGAGA ATGAGGCTGT GCCCAGAATC ACGAAAAAGT
    GCCAGGAAGC TATATGAAAG AGCTCACAAG CTACGCAAAA ATAATCACCA CCTCTTTACA
    ACTACCATTA ACTTGAATTC AATGAATGAA GGATGGTATG GAGCTCTAAA AGAAGCAATT
    CAGCAACAAC AAAACCAACT GGTGTGGGTT TCTGAAGGAA AGGCAGATGG TGCTACAAGT
    GATGACCTTG ATTTACATGA TGACCGCCTG TCTTACCTCT CAGCTCCTGG TAGTGAATAT
    TCTATGTACA GTACAGACAG CAGACATACA TCTGACTATG AAGACACAGA TACAGAAGGT
    GGTGCTTATA CTGATCAAGA ACTGGATGAA ACTCTGAACG ATGAGGTTGG AACTCCTCCT
    GAATCTGCTA TTACACGTTC TTCTGAACCC GTTAGGGAGG ATTCTTCTGG AATGCATCAT
    GAAACTCAAA CTTACACTAG TTACGCATCT CAAGCTCAGC CTCAGCCAAA TCTTAGAATA
    GATTCTTCAG GATTTAAAAC AACTGCTTCT CAGCCGGTAT ACAGAAAGGA TCCATATATT
    AGTGAAGAAG CATCCAGACA AAGCTATGTC TTAAAACAGC CAGCTGTTAA TCACCCAGTA
    CAAAGACAGG AAAGAGACCC AAATCTGATC TATGAATCTC AGACTCAGTA TGCAGAAAAA
    CAACCAAGTA GGGAGTACGA GCAGTCAACA TACAGGTATG ATTCTACAAA CTATGTAGAT
    CAATTTTCTC GTGGGTATGA CCCTCGTCTA CATTATGAAG ATCGTGCGCC TCCCTATGAG
    GAGCACTGGT CATATTATGA TGAAAAACAG CCCTACAATC AACCAAGAAC AGCTTATGAA
    AGCCAGCCTC CTAGAGATCT TGATTCCAGA CAAAATACAG AAGAGAGCAC AGAACGCAGC
    TATTATCCGG CACAACCCCG ATTTGAGGAA CCTCCTCCGA TGAGCTACGA TGGCAGACCT
    CGCTATGAGC ATGCACCCAA AAACTTCAGC TTGCCACAAG TGCGGTATGA AGATCAACAT
    CCTGTTGGCT ATGACACACA TGCGCATGGT AGATATAAAC AAGAAGCTCA GCCATACCAG
    TCAGCCATAT CTCGATCTCC TGAACCCAAG CAGTACTTTG ATCCACATGT GAGGGGCTAT
    GAGCAAGGTC CTCCTCAAGC TTACAATGCA AAAGCTGGAC AGTTTGAGCC TTCTCATAGC
    ACTTCAGGTG TTTCTCTTCC TCCTCCCGCT TCTTCACAAA CAAAACCAGA AGTTTTGCCT
    TCCAACAGTA AACCACTGCC TACACCACCA TCTCTAGCAG AAGAAGAGGA GGATCCAGCC
    ATGAAACCAC AGTCTGTGCG AAGTAGAGTT AAGATATTTG AACGAAGAAG ATCGCCATCT
    CTGGAAAAAA TGAAGGACCC CAGTGACACG TCAGTTGTCA AACCTCCAGA GCTAGCACCG
    AAGCCTACGC TCCCAGCCAT GAGTGGTCCA AAACCAACTT CTCAAAGCCA GTATGAACAT
    GATAAAACAA CTTACAGGGC CCCAGAACCA CAGAGACCTC AAGTGAAGCC ACCTGAAGAT
    ATCGTGCGTT CAAATCATTA TGATCCCGAA GAAGATGAAG AGTATTACAG AAAGCAGCTC
    TCATACTTTG ATCGCAGGAA TTTTGAAAAT AAGCCTTCTG CACAGGTTCC TGCCAGCCAT
    CATTCTGACT CCACTAAACC AATACATTCA CAAAATCAGC TGAATTTCTC CAATTATTCT
    AAGGGGAAGC CAACTGATGC TGAACCAATG GATAGATCTG TTGGTGACAA GCGCTATGAG
    CCAATCCCTC AAGTTACAAC TCCTCCTCCT GCTCCTTCAG TACAGTATAC ACAACCTCAG
    TCAATTAATA GTCCTGTCCT GTCTCTCCCA GCGCATCACA AGCCTGCACT TTCTGAAGTT
    AACTCTGTGT CTGACCCTCC TCCACCTCAG AATAAGCCAG CAATTTTCAG ATCCTCTAGA
    GAGGACACTG TGCAGTCTAC TTTTTATCCT CAGAAAAGCT TCCCTGACAA AGGTCCTGTT
    AATGGAACTG AACAGGTTCA GAAAACAGTC ACTCCTTCTT ACAACCGTTT TACAACAAAA
    CCTTACACTA GTGCTGCGAG GCCCTTTGAG CGCAAGTTTG AAAGTCCTAA ATTCAACCAT
    AATCTCTTGC CAAATGAAGC TCAACATAAA CCAGAGTTGC CATCAAAATC TCCAAATTCT
    CCTCAACCAA TTTTGAAAGC ACACAGCTCA TCACAGCCTC CTGAGTTTGA CAGTGGAATG
    GACACCTTCA CTGTACAGGT TGACAAGCCT AAATATCAAC CAAATAATGT AAATGCTGTG
    CCTAAAGCCA TTCCTGTAAG CCCTTCAGCA CTAGAGGATG AGGAGGAAGA AGACGGACAC
    ACTGTTGTAG CCACAGCAAG AGGTGTATTC AACAGCAATG GTGGCGTATT GAGCTCCATA
    GAGACTGGAG TTAGTATTAT TATACCACAA GGAGCCATTC CAGAGGGAAT AGAGCAAGAA
    ATATATTTTA AAGTCTGCAG AGACAACAGT ATACTTCCAC CTTTGGACAA AGAGAAAGGT
    GAAACACTTC TTAGCCCCTT GGTAATGTGT GGGCCTCACG GACTAAAATT CCTGAAGCCA
    GTGGAGCTGC GCCTGCCACA CTGTGACCCC AAAACCTGGC AGAACAAGTC TCTTCCTGGA
    GATCCAAACT ATCTTGTTGG AGCAAACTGT GTCTCAGTCC TAATTGACCA CTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa40350
    Clone ID Related Accession (Same CDS sequence) XM_015278975.1
    Accession Version XM_015278975.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5517bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product tight junction protein ZO-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763853.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGAAGTATC AGAAATACCT GACTGTCTTG CAGATGGCCA TAGGGGTGAC CGCCTCCAAC 
    AGGGGCAGTC TGATGCCCTT AAAGAGGAAG CTGTGGGTAA CTCCATCTTC CGAAAATCCC
    AATGGTGCTA CCTGTAGTGT AAGTCAGGGA AAGCCTTCTT TAAGAAGAAT AAAAGGGAGA
    ATACACAGAA GCAAAAGCCT TGATAGCATT GATTTCTGTG AATTCACTAG TGCAACAATG
    GAAGAAACAG CTATATGGGA ACAACACACG GTGACTCTTC ACAGGGCTCC TGGATTTGGG
    TTTGGTATTG CAATATCTGG AGGAAGGGAT AATCCTCACT TTCAAAGTGG TGAGACCTCA
    ATAGTTATTT CTGATGTGCT GAAAGGAGGC CCAGCAGAAG GACTGCTACA AGAAAATGAC
    CGTGTTGCAA TGGTTAATGG AGTTTCAATG GATAATGTTG AGCACGCATT TGCTGTTCAG
    CAGCTGAGGA AAAGTGGGAA GAATGCCAAA ATTACTATCC GAAGGATGAA GAAAATTCAG
    ATTCCAGTAG CTCGACAGGA ACCTGAGCCA GTGTCTGAAA ATGAGGACGA TAGCTATGAT
    GAAGAGATAC GGGATCCAAG GACCAGCCGT GGTGCTTCCA GTGCCAACAG AAGGCATGAG
    AAGAGCTGGG TACGAGACCG AAGTGCAAGC AGAGAAAGAA GCTTATCACC AAGATCTGAC
    AGAAGATCGG TTACTTCCAG TCAGCCTGCA AAACCTACCA AAGTAACGTT GGTGAAATCA
    AGGAAAAATG AAGAGTATGG TCTACGGTTG GCAAGCCACA TATTTGTGAA AGAAATATCA
    CAGGATAGCC TTGCAGCAAG AGATGGCAAT ATTCAGGAAG GGGATGTTGT TCTGAAGATA
    AATGGCACAG TGACAGAAAA TATGTCCTTA GCAGATGCAA AAACGCTAAT AGAAAGGTCC
    AAAGGCAAGT TGAAGATGGT GGTTCAGCGA GATGAACGAG CTACACTGTT GAATGTCCCT
    GATCTTTCTG ACAGTATTCA TTCTGCTAAT GCTTCGGAAA GAGATGACAT CTCAGAAATT
    CAGTCGCTGG CATCAGATCA TTCCAACCGA TCACATGACA GGCCCCGCCG CAGTCGTTCA
    CGATCTCCTG ACCAGAGGTC AGAGCCTTCA GACCATTCCA GACATTCTCC ACAGCAGCCC
    AGCAACGGCA GTCTTCGAAG CAGAGAAGAT GAAAGGGTAA CTAAACCAGG AGCCATCTCT
    ACACCTGTAA AGAATGCAGA TGATATTTCT AAAACTATGG AAGAGGTGTC AGTCGAAAGA
    ACTGAAAAAC AAACACCACC GCTCCCAGAA CCAAAGCCAG TATATGCACA AGGAGGTCAG
    CCAGATGTGG ATTTACCAGT CAGTCCGTCT GATGGTCCTT TACCCAATTC AACCCATGAA
    GATGGAATGC TTCGGCCAAG CATGAAACTG GTAAAATTCA GAAAAGGAGA TAGTGTGGGC
    CTGCGGCTCG CTGGTGGCAA TGATGTTGGC ATATTTGTAG CTGGTGTTCT AGAGGACAGC
    CCTGCAGCGA AAGAAGGATT AGAGGAAGGA GACCAAATTC TCAGGGTTAA TAACGTAGAC
    TTCACAAATA TCATCAGAGA AGAAGCTGTC CTTTTTTTGC TTGATCTCCC TAAAGGCGAA
    GAAGTAACCA TTTTGGCACA GAAGAAGAAA GATGTTTATC GTCGCATTGT TGAGTCTGAT
    GTAGGGGATT CTTTCTATAT CAGAACTCAT TTTGAATATG AAAAGGAATC TCCTTATGGA
    CTAAGCTTCA ACAAAGGTGA AGTGTTTCGG GTTGTGGACA CGCTATATAA TGGCAAACTG
    GGTTCGTGGC TGGCAATTCG AATTGGCAAG AATCATAAGG AAGTTGAAAG AGGTATCATA
    CCTAACAAAA ACAGAGCTGA ACAACTAGCT AGTGTACAGT ACACGCTTCC AAAGACAGCA
    GGAGGAGATC GAGCGGATTT CTGGAGATTC CGAGGTTTGC GTAGCTCAAA AAGAAATCTC
    CGAAAAAGTA GAGAAGATCT TTCTGCTCAG CCTGTTCAGA CAAAGTTCCC TGCCTATGAA
    AGAGTTGTTC TTCGGGAAGC TGGGTTTCTC AGACCTGTGA CCATTTTTGG TCCAATAGCT
    GATGTTGCAC GAGAGAAACT AGCCAGAGAA GAACCAGATA TCTTTCAAAT TGCAAAAAGT
    GAACCGAGAG ATGCTGGTAC TGACCAACGT AGTTCTGGCA TTATTCGTCT TCATACGATA
    AAACAGATAA TTGATAGAGA CAAACACGCT TTATTAGATG TCACTCCTAA TGCAGTGGAC
    CGTCTGAATT ATGCACAGTG GTATCCTATT GTAGTGTTTC TGAACCCTGA TTCTAAGCAG
    GGTGTGAAGA CCATGAGAAT GAGGCTGTGC CCAGAATCAC GAAAAAGTGC CAGGAAGCTA
    TATGAAAGAG CTCACAAGCT ACGCAAAAAT AATCACCACC TCTTTACAAC TACCATTAAC
    TTGAATTCAA TGAATGAAGG ATGGTATGGA GCTCTAAAAG AAGCAATTCA GCAACAACAA
    AACCAACTGG TGTGGGTTTC TGAAGGAAAG GCAGATGGTG CTACAAGTGA TGACCTTGAT
    TTACATGATG ACCGCCTGTC TTACCTCTCA GCTCCTGGTA GTGAATATTC TATGTACAGT
    ACAGACAGCA GACATACATC TGACTATGAA GACACAGATA CAGAAGGTGG TGCTTATACT
    GATCAAGAAC TGGATGAAAC TCTGAACGAT GAGGTTGGAA CTCCTCCTGA ATCTGCTATT
    ACACGTTCTT CTGAACCCGT TAGGGAGGAT TCTTCTGGAA TGCATCATGA AACTCAAACT
    TACACTAGTT ACGCATCTCA AGCTCAGCCT CAGCCAAATC TTAGAATAGA TTCTTCAGGA
    TTTAAAACAA CTGCTTCTCA GCCGAAAGCA GAAGCATCAC CTGCAGTCCC TTACCTTTCC
    CCGTCGCCTG AATCAAACCC AGCAACCTCA TCAACCTCTG CAGTAAATGC TAATGTAAAC
    CTAACTAATG TCAGACTAGA GGAGCCTACT GCTGCTCCTT ACAACTCTTA TCAACCACAA
    GCGGGCCCCT TAAGAACATC AAGTACTGAG GGAGCTCATG TAGTGCTAAG AGATCAAGAG
    CCATCATCCT TACCGCCGCA TATAGATCCA GCAAAGGTAT ACAGAAAGGA TCCATATATT
    AGTGAAGAAG CATCCAGACA AAGCTATGTC TTAAAACAGC CAGCTGTTAA TCACCCAGTA
    CAAAGACAGG AAAGAGACCC AAATCTGATC TATGAATCTC AGACTCAGTA TGCAGAAAAA
    CAACCAAGTA GGGAGTACGA GCAGTCAACA TACAGGTATG ATTCTACAAA CTATGTAGAT
    CAATTTTCTC GTGGGTATGA CCCTCGTCTA CATTATGAAG ATCGTGCGCC TCCCTATGAG
    GAGCACTGGT CATATTATGA TGAAAAACAG CCCTACAATC AACCAAGAAC AGCTTATGAA
    AGCCAGCCTC CTAGAGATCT TGATTCCAGA CAAAATACAG AAGAGAGCAC AGAACGCAGC
    TATTATCCGG CACAACCCCG ATTTGAGGAA CCTCCTCCGA TGAGCTACGA TGGCAGACCT
    CGCTATGAGC ATGCACCCAA AAACTTCAGC TTGCCACAAG TGCGGTATGA AGATCAACAT
    CCTGTTGGCT ATGACACACA TGCGCATGGT AGATATAAAC AAGAAGCTCA GCCATACCAG
    TCAGCCATAT CTCGATCTCC TGAACCCAAG CAGTACTTTG ATCCACATGT GAGGGGCTAT
    GAGCAAGGTC CTCCTCAAGC TTACAATGCA AAAGCTGGAC AGTTTGAGCC TTCTCATAGC
    ACTTCAGGTG TTTCTCTTCC TCCTCCCGCT TCTTCACAAA CAAAACCAGA AGTTTTGCCT
    TCCAACAGTA AACCACTGCC TACACCACCA TCTCTAGCAG AAGAAGAGGA GGATCCAGCC
    ATGAAACCAC AGTCTGTGCG AAGTAGAGTT AAGATATTTG AACGAAGAAG ATCGCCATCT
    CTGGAAAAAA TGAAGGACCC CAGTGACACG TCAGTTGTCA AACCTCCAGA GCTAGCACCG
    AAGCCTACGC TCCCAGCCAT GAGTGGTCCA AAACCAACTT CTCAAAGCCA GTATGAACAT
    GATAAAACAA CTTACAGGGC CCCAGAACCA CAGAGACCTC AAGTGAAGCC ACCTGAAGAT
    ATCGTGCGTT CAAATCATTA TGATCCCGAA GAAGATGAAG AGTATTACAG AAAGCAGCTC
    TCATACTTTG ATCGCAGGAA TTTTGAAAAT AAGCCTTCTG CACAGGTTCC TGCCAGCCAT
    CATTCTGACT CCACTAAACC AATACATTCA CAAAATCAGC TGAATTTCTC CAATTATTCT
    AAGGGGAAGC CAACTGATGC TGAACCAATG GATAGATCTG TTGGTGACAA GCGCTATGAG
    CCAATCCCTC AAGTTACAAC TCCTCCTCCT GCTCCTTCAG TACAGTATAC ACAACCTCAG
    TCAATTAATA GTCCTGTCCT GTCTCTCCCA GCGCATCACA AGCCTGCACT TTCTGAAGTT
    AACTCTGTGT CTGACCCTCC TCCACCTCAG AATAAGCCAG CAATTTTCAG ATCCTCTAGA
    GAGGACACTG TGCAGTCTAC TTTTTATCCT CAGAAAAGCT TCCCTGACAA AGGTCCTGTT
    AATGGAACTG AACAGGTTCA GAAAACAGTC ACTCCTTCTT ACAACCGTTT TACAACAAAA
    CCTTACACTA GTGCTGCGAG GCCCTTTGAG CGCAAGTTTG AAAGTCCTAA ATTCAACCAT
    AATCTCTTGC CAAATGAAGC TCAACATAAA CCAGAGTTGC CATCAAAATC TCCAAATTCT
    CCTCAACCAA TTTTGAAAGC ACACAGCTCA TCACAGCCTC CTGAGTTTGA CAGTGGAATG
    GACACCTTCA CTGTACAGGT TGACAAGCCT AAATATCAAC CAAATAATGT AAATGCTGTG
    CCTAAAGCCA TTCCTGTAAG CCCTTCAGCA CTAGAGGATG AGGAGGAAGA AGACGGACAC
    ACTGTTGTAG CCACAGCAAG AGGTGTATTC AACAGCAATG GTGGCGTATT GAGCTCCATA
    GAGACTGGAG TTAGTATTAT TATACCACAA GGAGCCATTC CAGAGGGAAT AGAGCAAGAA
    ATATATTTTA AAGTCTGCAG AGACAACAGT ATACTTCCAC CTTTGGACAA AGAGAAAGGT
    GAAACACTTC TTAGCCCCTT GGTAATGTGT GGGCCTCACG GACTAAAATT CCTGAAGCCA
    GTGGAGCTGC GCCTGCCACA CTGTGCGTCT ATGACCCCTG ATGGTTGGTC TTTTGCTCTA
    AAATCCTCTG ACTCCTCGTC GGGTGACCCC AAAACCTGGC AGAACAAGTC TCTTCCTGGA
    GATCCAAACT ATCTTGTTGG AGCAAACTGT GTCTCAGTCC TAATTGACCA CTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015134461.1
    CDS16..5532
    Translation

    Target ORF information:

    RefSeq Version XM_015278975.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus tight junction protein 1 (TJP1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015278975.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGAAGTATC AGAAATACCT GACTGTCTTG CAGATGGCCA TAGGGGTGAC CGCCTCCAAC 
    AGGGGCAGTC TGATGCCCTT AAAGAGGAAG CTGTGGGTAA CTCCATCTTC CGAAAATCCC
    AATGGTGCTA CCTGTAGTGT AAGTCAGGGA AAGCCTTCTT TAAGAAGAAT AAAAGGGAGA
    ATACACAGAA GCAAAAGCCT TGATAGCATT GATTTCTGTG AATTCACTAG TGCAACAATG
    GAAGAAACAG CTATATGGGA ACAACACACG GTGACTCTTC ACAGGGCTCC TGGATTTGGG
    TTTGGTATTG CAATATCTGG AGGAAGGGAT AATCCTCACT TTCAAAGTGG TGAGACCTCA
    ATAGTTATTT CTGATGTGCT GAAAGGAGGC CCAGCAGAAG GACTGCTACA AGAAAATGAC
    CGTGTTGCAA TGGTTAATGG AGTTTCAATG GATAATGTTG AGCACGCATT TGCTGTTCAG
    CAGCTGAGGA AAAGTGGGAA GAATGCCAAA ATTACTATCC GAAGGATGAA GAAAATTCAG
    ATTCCAGTAG CTCGACAGGA ACCTGAGCCA GTGTCTGAAA ATGAGGACGA TAGCTATGAT
    GAAGAGATAC GGGATCCAAG GACCAGCCGT GGTGCTTCCA GTGCCAACAG AAGGCATGAG
    AAGAGCTGGG TACGAGACCG AAGTGCAAGC AGAGAAAGAA GCTTATCACC AAGATCTGAC
    AGAAGATCGG TTACTTCCAG TCAGCCTGCA AAACCTACCA AAGTAACGTT GGTGAAATCA
    AGGAAAAATG AAGAGTATGG TCTACGGTTG GCAAGCCACA TATTTGTGAA AGAAATATCA
    CAGGATAGCC TTGCAGCAAG AGATGGCAAT ATTCAGGAAG GGGATGTTGT TCTGAAGATA
    AATGGCACAG TGACAGAAAA TATGTCCTTA GCAGATGCAA AAACGCTAAT AGAAAGGTCC
    AAAGGCAAGT TGAAGATGGT GGTTCAGCGA GATGAACGAG CTACACTGTT GAATGTCCCT
    GATCTTTCTG ACAGTATTCA TTCTGCTAAT GCTTCGGAAA GAGATGACAT CTCAGAAATT
    CAGTCGCTGG CATCAGATCA TTCCAACCGA TCACATGACA GGCCCCGCCG CAGTCGTTCA
    CGATCTCCTG ACCAGAGGTC AGAGCCTTCA GACCATTCCA GACATTCTCC ACAGCAGCCC
    AGCAACGGCA GTCTTCGAAG CAGAGAAGAT GAAAGGGTAA CTAAACCAGG AGCCATCTCT
    ACACCTGTAA AGAATGCAGA TGATATTTCT AAAACTATGG AAGAGGTGTC AGTCGAAAGA
    ACTGAAAAAC AAACACCACC GCTCCCAGAA CCAAAGCCAG TATATGCACA AGGAGGTCAG
    CCAGATGTGG ATTTACCAGT CAGTCCGTCT GATGGTCCTT TACCCAATTC AACCCATGAA
    GATGGAATGC TTCGGCCAAG CATGAAACTG GTAAAATTCA GAAAAGGAGA TAGTGTGGGC
    CTGCGGCTCG CTGGTGGCAA TGATGTTGGC ATATTTGTAG CTGGTGTTCT AGAGGACAGC
    CCTGCAGCGA AAGAAGGATT AGAGGAAGGA GACCAAATTC TCAGGGTTAA TAACGTAGAC
    TTCACAAATA TCATCAGAGA AGAAGCTGTC CTTTTTTTGC TTGATCTCCC TAAAGGCGAA
    GAAGTAACCA TTTTGGCACA GAAGAAGAAA GATGTTTATC GTCGCATTGT TGAGTCTGAT
    GTAGGGGATT CTTTCTATAT CAGAACTCAT TTTGAATATG AAAAGGAATC TCCTTATGGA
    CTAAGCTTCA ACAAAGGTGA AGTGTTTCGG GTTGTGGACA CGCTATATAA TGGCAAACTG
    GGTTCGTGGC TGGCAATTCG AATTGGCAAG AATCATAAGG AAGTTGAAAG AGGTATCATA
    CCTAACAAAA ACAGAGCTGA ACAACTAGCT AGTGTACAGT ACACGCTTCC AAAGACAGCA
    GGAGGAGATC GAGCGGATTT CTGGAGATTC CGAGGTTTGC GTAGCTCAAA AAGAAATCTC
    CGAAAAAGTA GAGAAGATCT TTCTGCTCAG CCTGTTCAGA CAAAGTTCCC TGCCTATGAA
    AGAGTTGTTC TTCGGGAAGC TGGGTTTCTC AGACCTGTGA CCATTTTTGG TCCAATAGCT
    GATGTTGCAC GAGAGAAACT AGCCAGAGAA GAACCAGATA TCTTTCAAAT TGCAAAAAGT
    GAACCGAGAG ATGCTGGTAC TGACCAACGT AGTTCTGGCA TTATTCGTCT TCATACGATA
    AAACAGATAA TTGATAGAGA CAAACACGCT TTATTAGATG TCACTCCTAA TGCAGTGGAC
    CGTCTGAATT ATGCACAGTG GTATCCTATT GTAGTGTTTC TGAACCCTGA TTCTAAGCAG
    GGTGTGAAGA CCATGAGAAT GAGGCTGTGC CCAGAATCAC GAAAAAGTGC CAGGAAGCTA
    TATGAAAGAG CTCACAAGCT ACGCAAAAAT AATCACCACC TCTTTACAAC TACCATTAAC
    TTGAATTCAA TGAATGAAGG ATGGTATGGA GCTCTAAAAG AAGCAATTCA GCAACAACAA
    AACCAACTGG TGTGGGTTTC TGAAGGAAAG GCAGATGGTG CTACAAGTGA TGACCTTGAT
    TTACATGATG ACCGCCTGTC TTACCTCTCA GCTCCTGGTA GTGAATATTC TATGTACAGT
    ACAGACAGCA GACATACATC TGACTATGAA GACACAGATA CAGAAGGTGG TGCTTATACT
    GATCAAGAAC TGGATGAAAC TCTGAACGAT GAGGTTGGAA CTCCTCCTGA ATCTGCTATT
    ACACGTTCTT CTGAACCCGT TAGGGAGGAT TCTTCTGGAA TGCATCATGA AACTCAAACT
    TACACTAGTT ACGCATCTCA AGCTCAGCCT CAGCCAAATC TTAGAATAGA TTCTTCAGGA
    TTTAAAACAA CTGCTTCTCA GCCGAAAGCA GAAGCATCAC CTGCAGTCCC TTACCTTTCC
    CCGTCGCCTG AATCAAACCC AGCAACCTCA TCAACCTCTG CAGTAAATGC TAATGTAAAC
    CTAACTAATG TCAGACTAGA GGAGCCTACT GCTGCTCCTT ACAACTCTTA TCAACCACAA
    GCGGGCCCCT TAAGAACATC AAGTACTGAG GGAGCTCATG TAGTGCTAAG AGATCAAGAG
    CCATCATCCT TACCGCCGCA TATAGATCCA GCAAAGGTAT ACAGAAAGGA TCCATATATT
    AGTGAAGAAG CATCCAGACA AAGCTATGTC TTAAAACAGC CAGCTGTTAA TCACCCAGTA
    CAAAGACAGG AAAGAGACCC AAATCTGATC TATGAATCTC AGACTCAGTA TGCAGAAAAA
    CAACCAAGTA GGGAGTACGA GCAGTCAACA TACAGGTATG ATTCTACAAA CTATGTAGAT
    CAATTTTCTC GTGGGTATGA CCCTCGTCTA CATTATGAAG ATCGTGCGCC TCCCTATGAG
    GAGCACTGGT CATATTATGA TGAAAAACAG CCCTACAATC AACCAAGAAC AGCTTATGAA
    AGCCAGCCTC CTAGAGATCT TGATTCCAGA CAAAATACAG AAGAGAGCAC AGAACGCAGC
    TATTATCCGG CACAACCCCG ATTTGAGGAA CCTCCTCCGA TGAGCTACGA TGGCAGACCT
    CGCTATGAGC ATGCACCCAA AAACTTCAGC TTGCCACAAG TGCGGTATGA AGATCAACAT
    CCTGTTGGCT ATGACACACA TGCGCATGGT AGATATAAAC AAGAAGCTCA GCCATACCAG
    TCAGCCATAT CTCGATCTCC TGAACCCAAG CAGTACTTTG ATCCACATGT GAGGGGCTAT
    GAGCAAGGTC CTCCTCAAGC TTACAATGCA AAAGCTGGAC AGTTTGAGCC TTCTCATAGC
    ACTTCAGGTG TTTCTCTTCC TCCTCCCGCT TCTTCACAAA CAAAACCAGA AGTTTTGCCT
    TCCAACAGTA AACCACTGCC TACACCACCA TCTCTAGCAG AAGAAGAGGA GGATCCAGCC
    ATGAAACCAC AGTCTGTGCG AAGTAGAGTT AAGATATTTG AACGAAGAAG ATCGCCATCT
    CTGGAAAAAA TGAAGGACCC CAGTGACACG TCAGTTGTCA AACCTCCAGA GCTAGCACCG
    AAGCCTACGC TCCCAGCCAT GAGTGGTCCA AAACCAACTT CTCAAAGCCA GTATGAACAT
    GATAAAACAA CTTACAGGGC CCCAGAACCA CAGAGACCTC AAGTGAAGCC ACCTGAAGAT
    ATCGTGCGTT CAAATCATTA TGATCCCGAA GAAGATGAAG AGTATTACAG AAAGCAGCTC
    TCATACTTTG ATCGCAGGAA TTTTGAAAAT AAGCCTTCTG CACAGGTTCC TGCCAGCCAT
    CATTCTGACT CCACTAAACC AATACATTCA CAAAATCAGC TGAATTTCTC CAATTATTCT
    AAGGGGAAGC CAACTGATGC TGAACCAATG GATAGATCTG TTGGTGACAA GCGCTATGAG
    CCAATCCCTC AAGTTACAAC TCCTCCTCCT GCTCCTTCAG TACAGTATAC ACAACCTCAG
    TCAATTAATA GTCCTGTCCT GTCTCTCCCA GCGCATCACA AGCCTGCACT TTCTGAAGTT
    AACTCTGTGT CTGACCCTCC TCCACCTCAG AATAAGCCAG CAATTTTCAG ATCCTCTAGA
    GAGGACACTG TGCAGTCTAC TTTTTATCCT CAGAAAAGCT TCCCTGACAA AGGTCCTGTT
    AATGGAACTG AACAGGTTCA GAAAACAGTC ACTCCTTCTT ACAACCGTTT TACAACAAAA
    CCTTACACTA GTGCTGCGAG GCCCTTTGAG CGCAAGTTTG AAAGTCCTAA ATTCAACCAT
    AATCTCTTGC CAAATGAAGC TCAACATAAA CCAGAGTTGC CATCAAAATC TCCAAATTCT
    CCTCAACCAA TTTTGAAAGC ACACAGCTCA TCACAGCCTC CTGAGTTTGA CAGTGGAATG
    GACACCTTCA CTGTACAGGT TGACAAGCCT AAATATCAAC CAAATAATGT AAATGCTGTG
    CCTAAAGCCA TTCCTGTAAG CCCTTCAGCA CTAGAGGATG AGGAGGAAGA AGACGGACAC
    ACTGTTGTAG CCACAGCAAG AGGTGTATTC AACAGCAATG GTGGCGTATT GAGCTCCATA
    GAGACTGGAG TTAGTATTAT TATACCACAA GGAGCCATTC CAGAGGGAAT AGAGCAAGAA
    ATATATTTTA AAGTCTGCAG AGACAACAGT ATACTTCCAC CTTTGGACAA AGAGAAAGGT
    GAAACACTTC TTAGCCCCTT GGTAATGTGT GGGCCTCACG GACTAAAATT CCTGAAGCCA
    GTGGAGCTGC GCCTGCCACA CTGTGCGTCT ATGACCCCTG ATGGTTGGTC TTTTGCTCTA
    AAATCCTCTG ACTCCTCGTC GGGTGACCCC AAAACCTGGC AGAACAAGTC TCTTCCTGGA
    GATCCAAACT ATCTTGTTGG AGCAAACTGT GTCTCAGTCC TAATTGACCA CTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa40351
    Clone ID Related Accession (Same CDS sequence) XM_015278976.1
    Accession Version XM_015278976.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5478bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product tight junction protein ZO-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763853.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGAAGTATC AGAAATACCT GACTGTCTTG CAGATGGCCA TAGGGGTGAC CGCCTCCAAC 
    AGGGGCAGTC TGATGCCCTT AAAGAGGAAG CTGTGGGTAA CTCCATCTTC CGAAAATCCC
    AATGGTGCTA CCTGTAGTGT AAGTCAGGGA AAGCCTTCTT TAAGAAGAAT AAAAGGGAGA
    ATACACAGAA GCAAAAGCCT TGATAGCATT GATTTCTGTG AATTCACTAG TGCAACAATG
    GAAGAAACAG CTATATGGGA ACAACACACG GTGACTCTTC ACAGGGCTCC TGGATTTGGG
    TTTGGTATTG CAATATCTGG AGGAAGGGAT AATCCTCACT TTCAAAGTGG TGAGACCTCA
    ATAGTTATTT CTGATGTGCT GAAAGGAGGC CCAGCAGAAG GACTGCTACA AGAAAATGAC
    CGTGTTGCAA TGGTTAATGG AGTTTCAATG GATAATGTTG AGCACGCATT TGCTGTTCAG
    CAGCTGAGGA AAAGTGGGAA GAATGCCAAA ATTACTATCC GAAGGATGAA GAAAATTCAG
    ATTCCAGTAG CTCGACAGGA ACCTGAGCCA GTGTCTGAAA ATGAGGACGA TAGCTATGAT
    GAAGAGATAC GGGATCCAAG GACCAGCCGT GGTGCTTCCA GTGCCAACAG AAGGCATGAG
    AAGAGCTGGG TACGAGACCG AAGTGCAAGC AGAGAAAGAA GCTTATCACC AAGATCTGAC
    AGAAGATCGG TTACTTCCAG TCAGCCTGCA AAACCTACCA AAGTAACGTT GGTGAAATCA
    AGGAAAAATG AAGAGTATGG TCTACGGTTG GCAAGCCACA TATTTGTGAA AGAAATATCA
    CAGGATAGCC TTGCAGCAAG AGATGGCAAT ATTCAGGAAG GGGATGTTGT TCTGAAGATA
    AATGGCACAG TGACAGAAAA TATGTCCTTA GCAGATGCAA AAACGCTAAT AGAAAGGTCC
    AAAGGCAAGT TGAAGATGGT GGTTCAGCGA GATGAACGAG CTACACTGTT GAATGTCCCT
    GATCTTTCTG ACAGTATTCA TTCTGCTAAT GCTTCGGAAA GAGATGACAT CTCAGAAATT
    CAGTCGCTGG CATCAGATCA TTCCAACCGA TCACATGACA GGCCCCGCCG CAGTCGTTCA
    CGATCTCCTG ACCAGAGGTC AGAGCCTTCA GACCATTCCA GACATTCTCC ACAGCAGCCC
    AGCAACGGCA GTCTTCGAAG CAGAGAAGAT GAAAGGGTAA CTAAACCAGG AGCCATCTCT
    ACACCTGTAA AGAATGCAGA TGATATTTCT AAAACTATGG AAGAGGTGTC AGTCGAAAGA
    ACTGAAAAAC AAACACCACC GCTCCCAGAA CCAAAGCCAG TATATGCACA AGGAGGTCAG
    CCAGATGTGG ATTTACCAGT CAGTCCGTCT GATGGTCCTT TACCCAATTC AACCCATGAA
    GATGGAATGC TTCGGCCAAG CATGAAACTG GTAAAATTCA GAAAAGGAGA TAGTGTGGGC
    CTGCGGCTCG CTGGTGGCAA TGATGTTGGC ATATTTGTAG CTGGTGTTCT AGAGGACAGC
    CCTGCAGCGA AAGAAGGATT AGAGGAAGGA GACCAAATTC TCAGGGTTAA TAACGTAGAC
    TTCACAAATA TCATCAGAGA AGAAGCTGTC CTTTTTTTGC TTGATCTCCC TAAAGGCGAA
    GAAGTAACCA TTTTGGCACA GAAGAAGAAA GATGTTTATC GTCGCATTGT TGAGTCTGAT
    GTAGGGGATT CTTTCTATAT CAGAACTCAT TTTGAATATG AAAAGGAATC TCCTTATGGA
    CTAAGCTTCA ACAAAGGTGA AGTGTTTCGG GTTGTGGACA CGCTATATAA TGGCAAACTG
    GGTTCGTGGC TGGCAATTCG AATTGGCAAG AATCATAAGG AAGTTGAAAG AGGTATCATA
    CCTAACAAAA ACAGAGCTGA ACAACTAGCT AGTGTACAGT ACACGCTTCC AAAGACAGCA
    GGAGGAGATC GAGCGGATTT CTGGAGATTC CGAGGTTTGC GTAGCTCAAA AAGAAATCTC
    CGAAAAAGTA GAGAAGATCT TTCTGCTCAG CCTGTTCAGA CAAAGTTCCC TGCCTATGAA
    AGAGTTGTTC TTCGGGAAGC TGGGTTTCTC AGACCTGTGA CCATTTTTGG TCCAATAGCT
    GATGTTGCAC GAGAGAAACT AGCCAGAGAA GAACCAGATA TCTTTCAAAT TGCAAAAAGT
    GAACCGAGAG ATGCTGGTAC TGACCAACGT AGTTCTGGCA TTATTCGTCT TCATACGATA
    AAACAGATAA TTGATAGAGA CAAACACGCT TTATTAGATG TCACTCCTAA TGCAGTGGAC
    CGTCTGAATT ATGCACAGTG GTATCCTATT GTAGTGTTTC TGAACCCTGA TTCTAAGCAG
    GGTGTGAAGA CCATGAGAAT GAGGCTGTGC CCAGAATCAC GAAAAAGTGC CAGGAAGCTA
    TATGAAAGAG CTCACAAGCT ACGCAAAAAT AATCACCACC TCTTTACAAC TACCATTAAC
    TTGAATTCAA TGAATGAAGG ATGGTATGGA GCTCTAAAAG AAGCAATTCA GCAACAACAA
    AACCAACTGG TGTGGGTTTC TGAAGGAAAG GCAGATGGTG CTACAAGTGA TGACCTTGAT
    TTACATGATG ACCGCCTGTC TTACCTCTCA GCTCCTGGTA GTGAATATTC TATGTACAGT
    ACAGACAGCA GACATACATC TGACTATGAA GACACAGATA CAGAAGGTGG TGCTTATACT
    GATCAAGAAC TGGATGAAAC TCTGAACGAT GAGGTTGGAA CTCCTCCTGA ATCTGCTATT
    ACACGTTCTT CTGAACCCGT TAGGGAGGAT TCTTCTGGAA TGCATCATGA AACTCAAACT
    TACACTAGTT ACGCATCTCA AGCTCAGCCT CAGCCAAATC TTAGAATAGA TTCTTCAGGA
    TTTAAAACAA CTGCTTCTCA GCCGAAAGCA GAAGCATCAC CTGCAGTCCC TTACCTTTCC
    CCGTCGCCTG AATCAAACCC AGCAACCTCA TCAACCTCTG CAGTAAATGC TAATGTAAAC
    CTAACTAATG TCAGACTAGA GGAGCCTACT GCTGCTCCTT ACAACTCTTA TCAACCACAA
    GCGGGCCCCT TAAGAACATC AAGTACTGAG GGAGCTCATG TAGTGCTAAG AGATCAAGAG
    CCATCATCCT TACCGCCGCA TATAGATCCA GCAAAGGTAT ACAGAAAGGA TCCATATATT
    AGTGAAGAAG CATCCAGACA AAGCTATGTC TTAAAACAGC CAGCTGTTAA TCACCCAGTA
    CAAAGACAGG AAAGAGACCC AAATCTGATC TATGAATCTC AGACTCAGTA TGCAGAAAAA
    CAACCAAGTA GGGAGTACGA GCAGTCAACA TACAGGTATG ATTCTACAAA CTATGTAGAT
    CAATTTTCTC GTGGGTATGA CCCTCGTCTA CATTATGAAG ATCGTGCGCC TCCCTATGAG
    GAGCACTGGT CATATTATGA TGAAAAACAG CCCTACAATC AACCAAGAAC AGCTTATGAA
    AGCCAGCCTC CTAGAGATCT TGATTCCAGA CAAAATACAG AAGAGAGCAC AGAACGCAGC
    TATTATCCGG CACAACCCCG ATTTGAGGAA CCTCCTCCGA TGAGCTACGA TGGCAGACCT
    CGCTATGAGC ATGCACCCAA AAACTTCAGC TTGCCACAAG TGCGGTATGA AGATCAACAT
    CCTGTTGGCT ATGACACACA TGCGCATGGT AGATATAAAC AAGAAGCTCA GCCATACCAG
    TCAGCCATAT CTCGATCTCC TGAACCCAAG CAGTACTTTG ATCCACATGT GAGGGGCTAT
    GAGCAAGGTC CTCCTCAAGC TTACAATGCA AAAGCTGGAC AGTTTGAGCC TTCTCATAGC
    ACTTCAGGTG TTTCTCTTCC TCCTCCCGCT TCTTCACAAA CAAAACCAGA AGTTTTGCCT
    TCCAACAGTA AACCACTGCC TACACCACCA TCTCTAGCAG AAGAAGAGGA GGATCCAGCC
    ATGAAACCAC AGTCTGTGCG AAGTAGAGTT AAGATATTTG AACGAAGAAG ATCGCCATCT
    CTGGAAAAAA TGAAGGACCC CAGTGACACG TCAGTTGTCA AACCTCCAGA GCTAGCACCG
    AAGCCTACGC TCCCAGCCAT GAGTGGTCCA AAACCAACTT CTCAAAGCCA GTATGAACAT
    GATAAAACAA CTTACAGGGC CCCAGAACCA CAGAGACCTC AAGTGAAGCC ACCTGAAGAT
    ATCGTGCGTT CAAATCATTA TGATCCCGAA GAAGATGAAG AGTATTACAG AAAGCAGCTC
    TCATACTTTG ATCGCAGGAA TTTTGAAAAT AAGCCTTCTG CACAGGTTCC TGCCAGCCAT
    CATTCTGACT CCACTAAACC AATACATTCA CAAAATCAGC TGAATTTCTC CAATTATTCT
    AAGGGGAAGC CAACTGATGC TGAACCAATG GATAGATCTG TTGGTGACAA GCGCTATGAG
    CCAATCCCTC AAGTTACAAC TCCTCCTCCT GCTCCTTCAG TACAGTATAC ACAACCTCAG
    TCAATTAATA GTCCTGTCCT GTCTCTCCCA GCGCATCACA AGCCTGCACT TTCTGAAGTT
    AACTCTGTGT CTGACCCTCC TCCACCTCAG AATAAGCCAG CAATTTTCAG ATCCTCTAGA
    GAGGACACTG TGCAGTCTAC TTTTTATCCT CAGAAAAGCT TCCCTGACAA AGGTCCTGTT
    AATGGAACTG AACAGGTTCA GAAAACAGTC ACTCCTTCTT ACAACCGTTT TACAACAAAA
    CCTTACACTA GTGCTGCGAG GCCCTTTGAG CGCAAGTTTG AAAGTCCTAA ATTCAACCAT
    AATCTCTTGC CAAATGAAGC TCAACATAAA CCAGAGTTGC CATCAAAATC TCCAAATTCT
    CCTCAACCAA TTTTGAAAGC ACACAGCTCA TCACAGCCTC CTGAGTTTGA CAGTGGAATG
    GACACCTTCA CTGTACAGGT TGACAAGCCT AAATATCAAC CAAATAATGT AAATGCTGTG
    CCTAAAGCCA TTCCTGTAAG CCCTTCAGCA CTAGAGGATG AGGAGGAAGA AGACGGACAC
    ACTGTTGTAG CCACAGCAAG AGGTGTATTC AACAGCAATG GTGGCGTATT GAGCTCCATA
    GAGACTGGAG TTAGTATTAT TATACCACAA GGAGCCATTC CAGAGGGAAT AGAGCAAGAA
    ATATATTTTA AAGTCTGCAG AGACAACAGT ATACTTCCAC CTTTGGACAA AGAGAAAGGT
    GAAACACTTC TTAGCCCCTT GGTAATGTGT GGGCCTCACG GACTAAAATT CCTGAAGCCA
    GTGGAGCTGC GCCTGCCACA CTGTGCGTCT ATGACCCCTG ATGGTGACCC CAAAACCTGG
    CAGAACAAGT CTCTTCCTGG AGATCCAAAC TATCTTGTTG GAGCAAACTG TGTCTCAGTC
    CTAATTGACC ACTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015134462.1
    CDS16..5493
    Translation

    Target ORF information:

    RefSeq Version XM_015278976.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus tight junction protein 1 (TJP1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015278976.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGAAGTATC AGAAATACCT GACTGTCTTG CAGATGGCCA TAGGGGTGAC CGCCTCCAAC 
    AGGGGCAGTC TGATGCCCTT AAAGAGGAAG CTGTGGGTAA CTCCATCTTC CGAAAATCCC
    AATGGTGCTA CCTGTAGTGT AAGTCAGGGA AAGCCTTCTT TAAGAAGAAT AAAAGGGAGA
    ATACACAGAA GCAAAAGCCT TGATAGCATT GATTTCTGTG AATTCACTAG TGCAACAATG
    GAAGAAACAG CTATATGGGA ACAACACACG GTGACTCTTC ACAGGGCTCC TGGATTTGGG
    TTTGGTATTG CAATATCTGG AGGAAGGGAT AATCCTCACT TTCAAAGTGG TGAGACCTCA
    ATAGTTATTT CTGATGTGCT GAAAGGAGGC CCAGCAGAAG GACTGCTACA AGAAAATGAC
    CGTGTTGCAA TGGTTAATGG AGTTTCAATG GATAATGTTG AGCACGCATT TGCTGTTCAG
    CAGCTGAGGA AAAGTGGGAA GAATGCCAAA ATTACTATCC GAAGGATGAA GAAAATTCAG
    ATTCCAGTAG CTCGACAGGA ACCTGAGCCA GTGTCTGAAA ATGAGGACGA TAGCTATGAT
    GAAGAGATAC GGGATCCAAG GACCAGCCGT GGTGCTTCCA GTGCCAACAG AAGGCATGAG
    AAGAGCTGGG TACGAGACCG AAGTGCAAGC AGAGAAAGAA GCTTATCACC AAGATCTGAC
    AGAAGATCGG TTACTTCCAG TCAGCCTGCA AAACCTACCA AAGTAACGTT GGTGAAATCA
    AGGAAAAATG AAGAGTATGG TCTACGGTTG GCAAGCCACA TATTTGTGAA AGAAATATCA
    CAGGATAGCC TTGCAGCAAG AGATGGCAAT ATTCAGGAAG GGGATGTTGT TCTGAAGATA
    AATGGCACAG TGACAGAAAA TATGTCCTTA GCAGATGCAA AAACGCTAAT AGAAAGGTCC
    AAAGGCAAGT TGAAGATGGT GGTTCAGCGA GATGAACGAG CTACACTGTT GAATGTCCCT
    GATCTTTCTG ACAGTATTCA TTCTGCTAAT GCTTCGGAAA GAGATGACAT CTCAGAAATT
    CAGTCGCTGG CATCAGATCA TTCCAACCGA TCACATGACA GGCCCCGCCG CAGTCGTTCA
    CGATCTCCTG ACCAGAGGTC AGAGCCTTCA GACCATTCCA GACATTCTCC ACAGCAGCCC
    AGCAACGGCA GTCTTCGAAG CAGAGAAGAT GAAAGGGTAA CTAAACCAGG AGCCATCTCT
    ACACCTGTAA AGAATGCAGA TGATATTTCT AAAACTATGG AAGAGGTGTC AGTCGAAAGA
    ACTGAAAAAC AAACACCACC GCTCCCAGAA CCAAAGCCAG TATATGCACA AGGAGGTCAG
    CCAGATGTGG ATTTACCAGT CAGTCCGTCT GATGGTCCTT TACCCAATTC AACCCATGAA
    GATGGAATGC TTCGGCCAAG CATGAAACTG GTAAAATTCA GAAAAGGAGA TAGTGTGGGC
    CTGCGGCTCG CTGGTGGCAA TGATGTTGGC ATATTTGTAG CTGGTGTTCT AGAGGACAGC
    CCTGCAGCGA AAGAAGGATT AGAGGAAGGA GACCAAATTC TCAGGGTTAA TAACGTAGAC
    TTCACAAATA TCATCAGAGA AGAAGCTGTC CTTTTTTTGC TTGATCTCCC TAAAGGCGAA
    GAAGTAACCA TTTTGGCACA GAAGAAGAAA GATGTTTATC GTCGCATTGT TGAGTCTGAT
    GTAGGGGATT CTTTCTATAT CAGAACTCAT TTTGAATATG AAAAGGAATC TCCTTATGGA
    CTAAGCTTCA ACAAAGGTGA AGTGTTTCGG GTTGTGGACA CGCTATATAA TGGCAAACTG
    GGTTCGTGGC TGGCAATTCG AATTGGCAAG AATCATAAGG AAGTTGAAAG AGGTATCATA
    CCTAACAAAA ACAGAGCTGA ACAACTAGCT AGTGTACAGT ACACGCTTCC AAAGACAGCA
    GGAGGAGATC GAGCGGATTT CTGGAGATTC CGAGGTTTGC GTAGCTCAAA AAGAAATCTC
    CGAAAAAGTA GAGAAGATCT TTCTGCTCAG CCTGTTCAGA CAAAGTTCCC TGCCTATGAA
    AGAGTTGTTC TTCGGGAAGC TGGGTTTCTC AGACCTGTGA CCATTTTTGG TCCAATAGCT
    GATGTTGCAC GAGAGAAACT AGCCAGAGAA GAACCAGATA TCTTTCAAAT TGCAAAAAGT
    GAACCGAGAG ATGCTGGTAC TGACCAACGT AGTTCTGGCA TTATTCGTCT TCATACGATA
    AAACAGATAA TTGATAGAGA CAAACACGCT TTATTAGATG TCACTCCTAA TGCAGTGGAC
    CGTCTGAATT ATGCACAGTG GTATCCTATT GTAGTGTTTC TGAACCCTGA TTCTAAGCAG
    GGTGTGAAGA CCATGAGAAT GAGGCTGTGC CCAGAATCAC GAAAAAGTGC CAGGAAGCTA
    TATGAAAGAG CTCACAAGCT ACGCAAAAAT AATCACCACC TCTTTACAAC TACCATTAAC
    TTGAATTCAA TGAATGAAGG ATGGTATGGA GCTCTAAAAG AAGCAATTCA GCAACAACAA
    AACCAACTGG TGTGGGTTTC TGAAGGAAAG GCAGATGGTG CTACAAGTGA TGACCTTGAT
    TTACATGATG ACCGCCTGTC TTACCTCTCA GCTCCTGGTA GTGAATATTC TATGTACAGT
    ACAGACAGCA GACATACATC TGACTATGAA GACACAGATA CAGAAGGTGG TGCTTATACT
    GATCAAGAAC TGGATGAAAC TCTGAACGAT GAGGTTGGAA CTCCTCCTGA ATCTGCTATT
    ACACGTTCTT CTGAACCCGT TAGGGAGGAT TCTTCTGGAA TGCATCATGA AACTCAAACT
    TACACTAGTT ACGCATCTCA AGCTCAGCCT CAGCCAAATC TTAGAATAGA TTCTTCAGGA
    TTTAAAACAA CTGCTTCTCA GCCGAAAGCA GAAGCATCAC CTGCAGTCCC TTACCTTTCC
    CCGTCGCCTG AATCAAACCC AGCAACCTCA TCAACCTCTG CAGTAAATGC TAATGTAAAC
    CTAACTAATG TCAGACTAGA GGAGCCTACT GCTGCTCCTT ACAACTCTTA TCAACCACAA
    GCGGGCCCCT TAAGAACATC AAGTACTGAG GGAGCTCATG TAGTGCTAAG AGATCAAGAG
    CCATCATCCT TACCGCCGCA TATAGATCCA GCAAAGGTAT ACAGAAAGGA TCCATATATT
    AGTGAAGAAG CATCCAGACA AAGCTATGTC TTAAAACAGC CAGCTGTTAA TCACCCAGTA
    CAAAGACAGG AAAGAGACCC AAATCTGATC TATGAATCTC AGACTCAGTA TGCAGAAAAA
    CAACCAAGTA GGGAGTACGA GCAGTCAACA TACAGGTATG ATTCTACAAA CTATGTAGAT
    CAATTTTCTC GTGGGTATGA CCCTCGTCTA CATTATGAAG ATCGTGCGCC TCCCTATGAG
    GAGCACTGGT CATATTATGA TGAAAAACAG CCCTACAATC AACCAAGAAC AGCTTATGAA
    AGCCAGCCTC CTAGAGATCT TGATTCCAGA CAAAATACAG AAGAGAGCAC AGAACGCAGC
    TATTATCCGG CACAACCCCG ATTTGAGGAA CCTCCTCCGA TGAGCTACGA TGGCAGACCT
    CGCTATGAGC ATGCACCCAA AAACTTCAGC TTGCCACAAG TGCGGTATGA AGATCAACAT
    CCTGTTGGCT ATGACACACA TGCGCATGGT AGATATAAAC AAGAAGCTCA GCCATACCAG
    TCAGCCATAT CTCGATCTCC TGAACCCAAG CAGTACTTTG ATCCACATGT GAGGGGCTAT
    GAGCAAGGTC CTCCTCAAGC TTACAATGCA AAAGCTGGAC AGTTTGAGCC TTCTCATAGC
    ACTTCAGGTG TTTCTCTTCC TCCTCCCGCT TCTTCACAAA CAAAACCAGA AGTTTTGCCT
    TCCAACAGTA AACCACTGCC TACACCACCA TCTCTAGCAG AAGAAGAGGA GGATCCAGCC
    ATGAAACCAC AGTCTGTGCG AAGTAGAGTT AAGATATTTG AACGAAGAAG ATCGCCATCT
    CTGGAAAAAA TGAAGGACCC CAGTGACACG TCAGTTGTCA AACCTCCAGA GCTAGCACCG
    AAGCCTACGC TCCCAGCCAT GAGTGGTCCA AAACCAACTT CTCAAAGCCA GTATGAACAT
    GATAAAACAA CTTACAGGGC CCCAGAACCA CAGAGACCTC AAGTGAAGCC ACCTGAAGAT
    ATCGTGCGTT CAAATCATTA TGATCCCGAA GAAGATGAAG AGTATTACAG AAAGCAGCTC
    TCATACTTTG ATCGCAGGAA TTTTGAAAAT AAGCCTTCTG CACAGGTTCC TGCCAGCCAT
    CATTCTGACT CCACTAAACC AATACATTCA CAAAATCAGC TGAATTTCTC CAATTATTCT
    AAGGGGAAGC CAACTGATGC TGAACCAATG GATAGATCTG TTGGTGACAA GCGCTATGAG
    CCAATCCCTC AAGTTACAAC TCCTCCTCCT GCTCCTTCAG TACAGTATAC ACAACCTCAG
    TCAATTAATA GTCCTGTCCT GTCTCTCCCA GCGCATCACA AGCCTGCACT TTCTGAAGTT
    AACTCTGTGT CTGACCCTCC TCCACCTCAG AATAAGCCAG CAATTTTCAG ATCCTCTAGA
    GAGGACACTG TGCAGTCTAC TTTTTATCCT CAGAAAAGCT TCCCTGACAA AGGTCCTGTT
    AATGGAACTG AACAGGTTCA GAAAACAGTC ACTCCTTCTT ACAACCGTTT TACAACAAAA
    CCTTACACTA GTGCTGCGAG GCCCTTTGAG CGCAAGTTTG AAAGTCCTAA ATTCAACCAT
    AATCTCTTGC CAAATGAAGC TCAACATAAA CCAGAGTTGC CATCAAAATC TCCAAATTCT
    CCTCAACCAA TTTTGAAAGC ACACAGCTCA TCACAGCCTC CTGAGTTTGA CAGTGGAATG
    GACACCTTCA CTGTACAGGT TGACAAGCCT AAATATCAAC CAAATAATGT AAATGCTGTG
    CCTAAAGCCA TTCCTGTAAG CCCTTCAGCA CTAGAGGATG AGGAGGAAGA AGACGGACAC
    ACTGTTGTAG CCACAGCAAG AGGTGTATTC AACAGCAATG GTGGCGTATT GAGCTCCATA
    GAGACTGGAG TTAGTATTAT TATACCACAA GGAGCCATTC CAGAGGGAAT AGAGCAAGAA
    ATATATTTTA AAGTCTGCAG AGACAACAGT ATACTTCCAC CTTTGGACAA AGAGAAAGGT
    GAAACACTTC TTAGCCCCTT GGTAATGTGT GGGCCTCACG GACTAAAATT CCTGAAGCCA
    GTGGAGCTGC GCCTGCCACA CTGTGCGTCT ATGACCCCTG ATGGTGACCC CAAAACCTGG
    CAGAACAAGT CTCTTCCTGG AGATCCAAAC TATCTTGTTG GAGCAAACTG TGTCTCAGTC
    CTAATTGACC ACTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa40352
    Clone ID Related Accession (Same CDS sequence) XM_015278977.1
    Accession Version XM_015278977.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5457bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product tight junction protein ZO-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763853.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGAAGTATC AGAAATACCT GACTGTCTTG CAGATGGCCA TAGGGGTGAC CGCCTCCAAC 
    AGGGGCAGTC TGATGCCCTT AAAGAGGAAG CTGTGGGTAA CTCCATCTTC CGAAAATCCC
    AATGGTGCTA CCTGTAGTGT AAGTCAGGGA AAGCCTTCTT TAAGAAGAAT AAAAGGGAGA
    ATACACAGAA GCAAAAGCCT TGATAGCATT GATTTCTGTG AATTCACTAG TGCAACAATG
    GAAGAAACAG CTATATGGGA ACAACACACG GTGACTCTTC ACAGGGCTCC TGGATTTGGG
    TTTGGTATTG CAATATCTGG AGGAAGGGAT AATCCTCACT TTCAAAGTGG TGAGACCTCA
    ATAGTTATTT CTGATGTGCT GAAAGGAGGC CCAGCAGAAG GACTGCTACA AGAAAATGAC
    CGTGTTGCAA TGGTTAATGG AGTTTCAATG GATAATGTTG AGCACGCATT TGCTGTTCAG
    CAGCTGAGGA AAAGTGGGAA GAATGCCAAA ATTACTATCC GAAGGATGAA GAAAATTCAG
    ATTCCAGTAG CTCGACAGGA ACCTGAGCCA GTGTCTGAAA ATGAGGACGA TAGCTATGAT
    GAAGAGATAC GGGATCCAAG GACCAGCCGT GGTGCTTCCA GTGCCAACAG AAGGCATGAG
    AAGAGCTGGG TACGAGACCG AAGTGCAAGC AGAGAAAGAA GCTTATCACC AAGATCTGAC
    AGAAGATCGG TTACTTCCAG TCAGCCTGCA AAACCTACCA AAGTAACGTT GGTGAAATCA
    AGGAAAAATG AAGAGTATGG TCTACGGTTG GCAAGCCACA TATTTGTGAA AGAAATATCA
    CAGGATAGCC TTGCAGCAAG AGATGGCAAT ATTCAGGAAG GGGATGTTGT TCTGAAGATA
    AATGGCACAG TGACAGAAAA TATGTCCTTA GCAGATGCAA AAACGCTAAT AGAAAGGTCC
    AAAGGCAAGT TGAAGATGGT GGTTCAGCGA GATGAACGAG CTACACTGTT GAATGTCCCT
    GATCTTTCTG ACAGTATTCA TTCTGCTAAT GCTTCGGAAA GAGATGACAT CTCAGAAATT
    CAGTCGCTGG CATCAGATCA TTCCAACCGA TCACATGACA GGCCCCGCCG CAGTCGTTCA
    CGATCTCCTG ACCAGAGGTC AGAGCCTTCA GACCATTCCA GACATTCTCC ACAGCAGCCC
    AGCAACGGCA GTCTTCGAAG CAGAGAAGAT GAAAGGGTAA CTAAACCAGG AGCCATCTCT
    ACACCTGTAA AGAATGCAGA TGATATTTCT AAAACTATGG AAGAGGTGTC AGTCGAAAGA
    ACTGAAAAAC AAACACCACC GCTCCCAGAA CCAAAGCCAG TATATGCACA AGGAGGTCAG
    CCAGATGTGG ATTTACCAGT CAGTCCGTCT GATGGTCCTT TACCCAATTC AACCCATGAA
    GATGGAATGC TTCGGCCAAG CATGAAACTG GTAAAATTCA GAAAAGGAGA TAGTGTGGGC
    CTGCGGCTCG CTGGTGGCAA TGATGTTGGC ATATTTGTAG CTGGTGTTCT AGAGGACAGC
    CCTGCAGCGA AAGAAGGATT AGAGGAAGGA GACCAAATTC TCAGGGTTAA TAACGTAGAC
    TTCACAAATA TCATCAGAGA AGAAGCTGTC CTTTTTTTGC TTGATCTCCC TAAAGGCGAA
    GAAGTAACCA TTTTGGCACA GAAGAAGAAA GATGTTTATC GTCGCATTGT TGAGTCTGAT
    GTAGGGGATT CTTTCTATAT CAGAACTCAT TTTGAATATG AAAAGGAATC TCCTTATGGA
    CTAAGCTTCA ACAAAGGTGA AGTGTTTCGG GTTGTGGACA CGCTATATAA TGGCAAACTG
    GGTTCGTGGC TGGCAATTCG AATTGGCAAG AATCATAAGG AAGTTGAAAG AGGTATCATA
    CCTAACAAAA ACAGAGCTGA ACAACTAGCT AGTGTACAGT ACACGCTTCC AAAGACAGCA
    GGAGGAGATC GAGCGGATTT CTGGAGATTC CGAGGTTTGC GTAGCTCAAA AAGAAATCTC
    CGAAAAAGTA GAGAAGATCT TTCTGCTCAG CCTGTTCAGA CAAAGTTCCC TGCCTATGAA
    AGAGTTGTTC TTCGGGAAGC TGGGTTTCTC AGACCTGTGA CCATTTTTGG TCCAATAGCT
    GATGTTGCAC GAGAGAAACT AGCCAGAGAA GAACCAGATA TCTTTCAAAT TGCAAAAAGT
    GAACCGAGAG ATGCTGGTAC TGACCAACGT AGTTCTGGCA TTATTCGTCT TCATACGATA
    AAACAGATAA TTGATAGAGA CAAACACGCT TTATTAGATG TCACTCCTAA TGCAGTGGAC
    CGTCTGAATT ATGCACAGTG GTATCCTATT GTAGTGTTTC TGAACCCTGA TTCTAAGCAG
    GGTGTGAAGA CCATGAGAAT GAGGCTGTGC CCAGAATCAC GAAAAAGTGC CAGGAAGCTA
    TATGAAAGAG CTCACAAGCT ACGCAAAAAT AATCACCACC TCTTTACAAC TACCATTAAC
    TTGAATTCAA TGAATGAAGG ATGGTATGGA GCTCTAAAAG AAGCAATTCA GCAACAACAA
    AACCAACTGG TGTGGGTTTC TGAAGGAAAG GCAGATGGTG CTACAAGTGA TGACCTTGAT
    TTACATGATG ACCGCCTGTC TTACCTCTCA GCTCCTGGTA GTGAATATTC TATGTACAGT
    ACAGACAGCA GACATACATC TGACTATGAA GACACAGATA CAGAAGGTGG TGCTTATACT
    GATCAAGAAC TGGATGAAAC TCTGAACGAT GAGGTTGGAA CTCCTCCTGA ATCTGCTATT
    ACACGTTCTT CTGAACCCGT TAGGGAGGAT TCTTCTGGAA TGCATCATGA AACTCAAACT
    TACACTAGTT ACGCATCTCA AGCTCAGCCT CAGCCAAATC TTAGAATAGA TTCTTCAGGA
    TTTAAAACAA CTGCTTCTCA GCCGAAAGCA GAAGCATCAC CTGCAGTCCC TTACCTTTCC
    CCGTCGCCTG AATCAAACCC AGCAACCTCA TCAACCTCTG CAGTAAATGC TAATGTAAAC
    CTAACTAATG TCAGACTAGA GGAGCCTACT GCTGCTCCTT ACAACTCTTA TCAACCACAA
    GCGGGCCCCT TAAGAACATC AAGTACTGAG GGAGCTCATG TAGTGCTAAG AGATCAAGAG
    CCATCATCCT TACCGCCGCA TATAGATCCA GCAAAGGTAT ACAGAAAGGA TCCATATATT
    AGTGAAGAAG CATCCAGACA AAGCTATGTC TTAAAACAGC CAGCTGTTAA TCACCCAGTA
    CAAAGACAGG AAAGAGACCC AAATCTGATC TATGAATCTC AGACTCAGTA TGCAGAAAAA
    CAACCAAGTA GGGAGTACGA GCAGTCAACA TACAGGTATG ATTCTACAAA CTATGTAGAT
    CAATTTTCTC GTGGGTATGA CCCTCGTCTA CATTATGAAG ATCGTGCGCC TCCCTATGAG
    GAGCACTGGT CATATTATGA TGAAAAACAG CCCTACAATC AACCAAGAAC AGCTTATGAA
    AGCCAGCCTC CTAGAGATCT TGATTCCAGA CAAAATACAG AAGAGAGCAC AGAACGCAGC
    TATTATCCGG CACAACCCCG ATTTGAGGAA CCTCCTCCGA TGAGCTACGA TGGCAGACCT
    CGCTATGAGC ATGCACCCAA AAACTTCAGC TTGCCACAAG TGCGGTATGA AGATCAACAT
    CCTGTTGGCT ATGACACACA TGCGCATGGT AGATATAAAC AAGAAGCTCA GCCATACCAG
    TCAGCCATAT CTCGATCTCC TGAACCCAAG CAGTACTTTG ATCCACATGT GAGGGGCTAT
    GAGCAAGGTC CTCCTCAAGC TTACAATGCA AAAGCTGGAC AGTTTGAGCC TTCTCATAGC
    ACTTCAGGTG TTTCTCTTCC TCCTCCCGCT TCTTCACAAA CAAAACCAGA AGTTTTGCCT
    TCCAACAGTA AACCACTGCC TACACCACCA TCTCTAGCAG AAGAAGAGGA GGATCCAGCC
    ATGAAACCAC AGTCTGTGCG AAGTAGAGTT AAGATATTTG AACGAAGAAG ATCGCCATCT
    CTGGAAAAAA TGAAGGACCC CAGTGACACG TCAGTTGTCA AACCTCCAGA GCTAGCACCG
    AAGCCTACGC TCCCAGCCAT GAGTGGTCCA AAACCAACTT CTCAAAGCCA GTATGAACAT
    GATAAAACAA CTTACAGGGC CCCAGAACCA CAGAGACCTC AAGTGAAGCC ACCTGAAGAT
    ATCGTGCGTT CAAATCATTA TGATCCCGAA GAAGATGAAG AGTATTACAG AAAGCAGCTC
    TCATACTTTG ATCGCAGGAA TTTTGAAAAT AAGCCTTCTG CACAGGTTCC TGCCAGCCAT
    CATTCTGACT CCACTAAACC AATACATTCA CAAAATCAGC TGAATTTCTC CAATTATTCT
    AAGGGGAAGC CAACTGATGC TGAACCAATG GATAGATCTG TTGGTGACAA GCGCTATGAG
    CCAATCCCTC AAGTTACAAC TCCTCCTCCT GCTCCTTCAG TACAGTATAC ACAACCTCAG
    TCAATTAATA GTCCTGTCCT GTCTCTCCCA GCGCATCACA AGCCTGCACT TTCTGAAGTT
    AACTCTGTGT CTGACCCTCC TCCACCTCAG AATAAGCCAG CAATTTTCAG ATCCTCTAGA
    GAGGACACTG TGCAGTCTAC TTTTTATCCT CAGAAAAGCT TCCCTGACAA AGGTCCTGTT
    AATGGAACTG AACAGGTTCA GAAAACAGTC ACTCCTTCTT ACAACCGTTT TACAACAAAA
    CCTTACACTA GTGCTGCGAG GCCCTTTGAG CGCAAGTTTG AAAGTCCTAA ATTCAACCAT
    AATCTCTTGC CAAATGAAGC TCAACATAAA CCAGAGTTGC CATCAAAATC TCCAAATTCT
    CCTCAACCAA TTTTGAAAGC ACACAGCTCA TCACAGCCTC CTGAGTTTGA CAGTGGAATG
    GACACCTTCA CTGTACAGGT TGACAAGCCT AAATATCAAC CAAATAATGT AAATGCTGTG
    CCTAAAGCCA TTCCTGTAAG CCCTTCAGCA CTAGAGGATG AGGAGGAAGA AGACGGACAC
    ACTGTTGTAG CCACAGCAAG AGGTGTATTC AACAGCAATG GTGGCGTATT GAGCTCCATA
    GAGACTGGAG TTAGTATTAT TATACCACAA GGAGCCATTC CAGAGGGAAT AGAGCAAGAA
    ATATATTTTA AAGTCTGCAG AGACAACAGT ATACTTCCAC CTTTGGACAA AGAGAAAGGT
    GAAACACTTC TTAGCCCCTT GGTAATGTGT GGGCCTCACG GACTAAAATT CCTGAAGCCA
    GTGGAGCTGC GCCTGCCACA CTGTGACCCC AAAACCTGGC AGAACAAGTC TCTTCCTGGA
    GATCCAAACT ATCTTGTTGG AGCAAACTGT GTCTCAGTCC TAATTGACCA CTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015134463.1
    CDS16..5472
    Translation

    Target ORF information:

    RefSeq Version XM_015278977.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus tight junction protein 1 (TJP1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015278977.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGAAGTATC AGAAATACCT GACTGTCTTG CAGATGGCCA TAGGGGTGAC CGCCTCCAAC 
    AGGGGCAGTC TGATGCCCTT AAAGAGGAAG CTGTGGGTAA CTCCATCTTC CGAAAATCCC
    AATGGTGCTA CCTGTAGTGT AAGTCAGGGA AAGCCTTCTT TAAGAAGAAT AAAAGGGAGA
    ATACACAGAA GCAAAAGCCT TGATAGCATT GATTTCTGTG AATTCACTAG TGCAACAATG
    GAAGAAACAG CTATATGGGA ACAACACACG GTGACTCTTC ACAGGGCTCC TGGATTTGGG
    TTTGGTATTG CAATATCTGG AGGAAGGGAT AATCCTCACT TTCAAAGTGG TGAGACCTCA
    ATAGTTATTT CTGATGTGCT GAAAGGAGGC CCAGCAGAAG GACTGCTACA AGAAAATGAC
    CGTGTTGCAA TGGTTAATGG AGTTTCAATG GATAATGTTG AGCACGCATT TGCTGTTCAG
    CAGCTGAGGA AAAGTGGGAA GAATGCCAAA ATTACTATCC GAAGGATGAA GAAAATTCAG
    ATTCCAGTAG CTCGACAGGA ACCTGAGCCA GTGTCTGAAA ATGAGGACGA TAGCTATGAT
    GAAGAGATAC GGGATCCAAG GACCAGCCGT GGTGCTTCCA GTGCCAACAG AAGGCATGAG
    AAGAGCTGGG TACGAGACCG AAGTGCAAGC AGAGAAAGAA GCTTATCACC AAGATCTGAC
    AGAAGATCGG TTACTTCCAG TCAGCCTGCA AAACCTACCA AAGTAACGTT GGTGAAATCA
    AGGAAAAATG AAGAGTATGG TCTACGGTTG GCAAGCCACA TATTTGTGAA AGAAATATCA
    CAGGATAGCC TTGCAGCAAG AGATGGCAAT ATTCAGGAAG GGGATGTTGT TCTGAAGATA
    AATGGCACAG TGACAGAAAA TATGTCCTTA GCAGATGCAA AAACGCTAAT AGAAAGGTCC
    AAAGGCAAGT TGAAGATGGT GGTTCAGCGA GATGAACGAG CTACACTGTT GAATGTCCCT
    GATCTTTCTG ACAGTATTCA TTCTGCTAAT GCTTCGGAAA GAGATGACAT CTCAGAAATT
    CAGTCGCTGG CATCAGATCA TTCCAACCGA TCACATGACA GGCCCCGCCG CAGTCGTTCA
    CGATCTCCTG ACCAGAGGTC AGAGCCTTCA GACCATTCCA GACATTCTCC ACAGCAGCCC
    AGCAACGGCA GTCTTCGAAG CAGAGAAGAT GAAAGGGTAA CTAAACCAGG AGCCATCTCT
    ACACCTGTAA AGAATGCAGA TGATATTTCT AAAACTATGG AAGAGGTGTC AGTCGAAAGA
    ACTGAAAAAC AAACACCACC GCTCCCAGAA CCAAAGCCAG TATATGCACA AGGAGGTCAG
    CCAGATGTGG ATTTACCAGT CAGTCCGTCT GATGGTCCTT TACCCAATTC AACCCATGAA
    GATGGAATGC TTCGGCCAAG CATGAAACTG GTAAAATTCA GAAAAGGAGA TAGTGTGGGC
    CTGCGGCTCG CTGGTGGCAA TGATGTTGGC ATATTTGTAG CTGGTGTTCT AGAGGACAGC
    CCTGCAGCGA AAGAAGGATT AGAGGAAGGA GACCAAATTC TCAGGGTTAA TAACGTAGAC
    TTCACAAATA TCATCAGAGA AGAAGCTGTC CTTTTTTTGC TTGATCTCCC TAAAGGCGAA
    GAAGTAACCA TTTTGGCACA GAAGAAGAAA GATGTTTATC GTCGCATTGT TGAGTCTGAT
    GTAGGGGATT CTTTCTATAT CAGAACTCAT TTTGAATATG AAAAGGAATC TCCTTATGGA
    CTAAGCTTCA ACAAAGGTGA AGTGTTTCGG GTTGTGGACA CGCTATATAA TGGCAAACTG
    GGTTCGTGGC TGGCAATTCG AATTGGCAAG AATCATAAGG AAGTTGAAAG AGGTATCATA
    CCTAACAAAA ACAGAGCTGA ACAACTAGCT AGTGTACAGT ACACGCTTCC AAAGACAGCA
    GGAGGAGATC GAGCGGATTT CTGGAGATTC CGAGGTTTGC GTAGCTCAAA AAGAAATCTC
    CGAAAAAGTA GAGAAGATCT TTCTGCTCAG CCTGTTCAGA CAAAGTTCCC TGCCTATGAA
    AGAGTTGTTC TTCGGGAAGC TGGGTTTCTC AGACCTGTGA CCATTTTTGG TCCAATAGCT
    GATGTTGCAC GAGAGAAACT AGCCAGAGAA GAACCAGATA TCTTTCAAAT TGCAAAAAGT
    GAACCGAGAG ATGCTGGTAC TGACCAACGT AGTTCTGGCA TTATTCGTCT TCATACGATA
    AAACAGATAA TTGATAGAGA CAAACACGCT TTATTAGATG TCACTCCTAA TGCAGTGGAC
    CGTCTGAATT ATGCACAGTG GTATCCTATT GTAGTGTTTC TGAACCCTGA TTCTAAGCAG
    GGTGTGAAGA CCATGAGAAT GAGGCTGTGC CCAGAATCAC GAAAAAGTGC CAGGAAGCTA
    TATGAAAGAG CTCACAAGCT ACGCAAAAAT AATCACCACC TCTTTACAAC TACCATTAAC
    TTGAATTCAA TGAATGAAGG ATGGTATGGA GCTCTAAAAG AAGCAATTCA GCAACAACAA
    AACCAACTGG TGTGGGTTTC TGAAGGAAAG GCAGATGGTG CTACAAGTGA TGACCTTGAT
    TTACATGATG ACCGCCTGTC TTACCTCTCA GCTCCTGGTA GTGAATATTC TATGTACAGT
    ACAGACAGCA GACATACATC TGACTATGAA GACACAGATA CAGAAGGTGG TGCTTATACT
    GATCAAGAAC TGGATGAAAC TCTGAACGAT GAGGTTGGAA CTCCTCCTGA ATCTGCTATT
    ACACGTTCTT CTGAACCCGT TAGGGAGGAT TCTTCTGGAA TGCATCATGA AACTCAAACT
    TACACTAGTT ACGCATCTCA AGCTCAGCCT CAGCCAAATC TTAGAATAGA TTCTTCAGGA
    TTTAAAACAA CTGCTTCTCA GCCGAAAGCA GAAGCATCAC CTGCAGTCCC TTACCTTTCC
    CCGTCGCCTG AATCAAACCC AGCAACCTCA TCAACCTCTG CAGTAAATGC TAATGTAAAC
    CTAACTAATG TCAGACTAGA GGAGCCTACT GCTGCTCCTT ACAACTCTTA TCAACCACAA
    GCGGGCCCCT TAAGAACATC AAGTACTGAG GGAGCTCATG TAGTGCTAAG AGATCAAGAG
    CCATCATCCT TACCGCCGCA TATAGATCCA GCAAAGGTAT ACAGAAAGGA TCCATATATT
    AGTGAAGAAG CATCCAGACA AAGCTATGTC TTAAAACAGC CAGCTGTTAA TCACCCAGTA
    CAAAGACAGG AAAGAGACCC AAATCTGATC TATGAATCTC AGACTCAGTA TGCAGAAAAA
    CAACCAAGTA GGGAGTACGA GCAGTCAACA TACAGGTATG ATTCTACAAA CTATGTAGAT
    CAATTTTCTC GTGGGTATGA CCCTCGTCTA CATTATGAAG ATCGTGCGCC TCCCTATGAG
    GAGCACTGGT CATATTATGA TGAAAAACAG CCCTACAATC AACCAAGAAC AGCTTATGAA
    AGCCAGCCTC CTAGAGATCT TGATTCCAGA CAAAATACAG AAGAGAGCAC AGAACGCAGC
    TATTATCCGG CACAACCCCG ATTTGAGGAA CCTCCTCCGA TGAGCTACGA TGGCAGACCT
    CGCTATGAGC ATGCACCCAA AAACTTCAGC TTGCCACAAG TGCGGTATGA AGATCAACAT
    CCTGTTGGCT ATGACACACA TGCGCATGGT AGATATAAAC AAGAAGCTCA GCCATACCAG
    TCAGCCATAT CTCGATCTCC TGAACCCAAG CAGTACTTTG ATCCACATGT GAGGGGCTAT
    GAGCAAGGTC CTCCTCAAGC TTACAATGCA AAAGCTGGAC AGTTTGAGCC TTCTCATAGC
    ACTTCAGGTG TTTCTCTTCC TCCTCCCGCT TCTTCACAAA CAAAACCAGA AGTTTTGCCT
    TCCAACAGTA AACCACTGCC TACACCACCA TCTCTAGCAG AAGAAGAGGA GGATCCAGCC
    ATGAAACCAC AGTCTGTGCG AAGTAGAGTT AAGATATTTG AACGAAGAAG ATCGCCATCT
    CTGGAAAAAA TGAAGGACCC CAGTGACACG TCAGTTGTCA AACCTCCAGA GCTAGCACCG
    AAGCCTACGC TCCCAGCCAT GAGTGGTCCA AAACCAACTT CTCAAAGCCA GTATGAACAT
    GATAAAACAA CTTACAGGGC CCCAGAACCA CAGAGACCTC AAGTGAAGCC ACCTGAAGAT
    ATCGTGCGTT CAAATCATTA TGATCCCGAA GAAGATGAAG AGTATTACAG AAAGCAGCTC
    TCATACTTTG ATCGCAGGAA TTTTGAAAAT AAGCCTTCTG CACAGGTTCC TGCCAGCCAT
    CATTCTGACT CCACTAAACC AATACATTCA CAAAATCAGC TGAATTTCTC CAATTATTCT
    AAGGGGAAGC CAACTGATGC TGAACCAATG GATAGATCTG TTGGTGACAA GCGCTATGAG
    CCAATCCCTC AAGTTACAAC TCCTCCTCCT GCTCCTTCAG TACAGTATAC ACAACCTCAG
    TCAATTAATA GTCCTGTCCT GTCTCTCCCA GCGCATCACA AGCCTGCACT TTCTGAAGTT
    AACTCTGTGT CTGACCCTCC TCCACCTCAG AATAAGCCAG CAATTTTCAG ATCCTCTAGA
    GAGGACACTG TGCAGTCTAC TTTTTATCCT CAGAAAAGCT TCCCTGACAA AGGTCCTGTT
    AATGGAACTG AACAGGTTCA GAAAACAGTC ACTCCTTCTT ACAACCGTTT TACAACAAAA
    CCTTACACTA GTGCTGCGAG GCCCTTTGAG CGCAAGTTTG AAAGTCCTAA ATTCAACCAT
    AATCTCTTGC CAAATGAAGC TCAACATAAA CCAGAGTTGC CATCAAAATC TCCAAATTCT
    CCTCAACCAA TTTTGAAAGC ACACAGCTCA TCACAGCCTC CTGAGTTTGA CAGTGGAATG
    GACACCTTCA CTGTACAGGT TGACAAGCCT AAATATCAAC CAAATAATGT AAATGCTGTG
    CCTAAAGCCA TTCCTGTAAG CCCTTCAGCA CTAGAGGATG AGGAGGAAGA AGACGGACAC
    ACTGTTGTAG CCACAGCAAG AGGTGTATTC AACAGCAATG GTGGCGTATT GAGCTCCATA
    GAGACTGGAG TTAGTATTAT TATACCACAA GGAGCCATTC CAGAGGGAAT AGAGCAAGAA
    ATATATTTTA AAGTCTGCAG AGACAACAGT ATACTTCCAC CTTTGGACAA AGAGAAAGGT
    GAAACACTTC TTAGCCCCTT GGTAATGTGT GGGCCTCACG GACTAAAATT CCTGAAGCCA
    GTGGAGCTGC GCCTGCCACA CTGTGACCCC AAAACCTGGC AGAACAAGTC TCTTCCTGGA
    GATCCAAACT ATCTTGTTGG AGCAAACTGT GTCTCAGTCC TAATTGACCA CTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa40354
    Clone ID Related Accession (Same CDS sequence) XM_015278979.1
    Accession Version XM_015278979.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5265bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product tight junction protein ZO-1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763853.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAAGTATC AGAAATACCT GACTGTCTTG CAGATGGCCA TAGGGGTGAC CGCCTCCAAC 
    AGGGGCAGTC TGATGCCCTT AAAGAGGAAG CTGTGGGTAA CTCCATCTTC CGAAAATCCC
    AATGGTGCTA CCTGTAGTGT AAGTCAGGGA AAGCCTTCTT TAAGAAGAAT AAAAGGGAGA
    ATACACAGAA GCAAAAGCCT TGATAGCATT GATTTCTGTG AATTCACTAG TGCAACAATG
    GAAGAAACAG CTATATGGGA ACAACACACG GTGACTCTTC ACAGGGCTCC TGGATTTGGG
    TTTGGTATTG CAATATCTGG AGGAAGGGAT AATCCTCACT TTCAAAGTGG TGAGACCTCA
    ATAGTTATTT CTGATGTGCT GAAAGGAGGC CCAGCAGAAG GACTGCTACA AGAAAATGAC
    CGTGTTGCAA TGGTTAATGG AGTTTCAATG GATAATGTTG AGCACGCATT TGCTGTTCAG
    CAGCTGAGGA AAAGTGGGAA GAATGCCAAA ATTACTATCC GAAGGATGAA GAAAATTCAG
    ATTCCAGTAG CTCGACAGGA ACCTGAGCCA GTGTCTGAAA ATGAGGACGA TAGCTATGAT
    GAAGAGATAC GGGATCCAAG GACCAGCCGT GGTGCTTCCA GTGCCAACAG AAGGCATGAG
    AAGAGCTGGG TACGAGACCG AAGTGCAAGC AGAGAAAGAA GCTTATCACC AAGATCTGAC
    AGAAGATCGG TTACTTCCAG TCAGCCTGCA AAACCTACCA AAGTAACGTT GGTGAAATCA
    AGGAAAAATG AAGAGTATGG TCTACGGTTG GCAAGCCACA TATTTGTGAA AGAAATATCA
    CAGGATAGCC TTGCAGCAAG AGATGGCAAT ATTCAGGAAG GGGATGTTGT TCTGAAGATA
    AATGGCACAG TGACAGAAAA TATGTCCTTA GCAGATGCAA AAACGCTAAT AGAAAGGTCC
    AAAGGCAAGT TGAAGATGGT GGTTCAGCGA GATGAACGAG CTACACTGTT GAATGTCCCT
    GATCTTTCTG ACAGTATTCA TTCTGCTAAT GCTTCGGAAA GAGATGACAT CTCAGAAATT
    CAGTCGCTGG CATCAGATCA TTCCAACCGA TCACATGACA GGCCCCGCCG CAGTCGTTCA
    CGATCTCCTG ACCAGAGGTC AGAGCCTTCA GACCATTCCA GACATTCTCC ACAGCAGCCC
    AGCAACGGCA GTCTTCGAAG CAGAGAAGAT GAAAGGGTAA CTAAACCAGG AGCCATCTCT
    ACACCTGTAA AGAATGCAGA TGATATTTCT AAAACTATGG AAGAGGTGTC AGTCGAAAGA
    ACTGAAAAAC AAACACCACC GCTCCCAGAA CCAAAGCCAG TATATGCACA AGGAGGTCAG
    CCAGATGTGG ATTTACCAGT CAGTCCGTCT GATGGTCCTT TACCCAATTC AACCCATGAA
    GATGGAATGC TTCGGCCAAG CATGAAACTG GTAAAATTCA GAAAAGGAGA TAGTGTGGGC
    CTGCGGCTCG CTGGTGGCAA TGATGTTGGC ATATTTGTAG CTGGTGTTCT AGAGGACAGC
    CCTGCAGCGA AAGAAGGATT AGAGGAAGGA GACCAAATTC TCAGGGTTAA TAACGTAGAC
    TTCACAAATA TCATCAGAGA AGAAGCTGTC CTTTTTTTGC TTGATCTCCC TAAAGGCGAA
    GAAGTAACCA TTTTGGCACA GAAGAAGAAA GATGTTTATC GTCGCATTGT TGAGTCTGAT
    GTAGGGGATT CTTTCTATAT CAGAACTCAT TTTGAATATG AAAAGGAATC TCCTTATGGA
    CTAAGCTTCA ACAAAGGTGA AGTGTTTCGG GTTGTGGACA CGCTATATAA TGGCAAACTG
    GGTTCGTGGC TGGCAATTCG AATTGGCAAG AATCATAAGG AAGTTGAAAG AGGTATCATA
    CCTAACAAAA ACAGAGCTGA ACAACTAGCT AGTGTACAGT ACACGCTTCC AAAGACAGCA
    GGAGGAGATC GAGCGGATTT CTGGAGATTC CGAGGTTTGC GTAGCTCAAA AAGAAATCTC
    CGAAAAAGTA GAGAAGATCT TTCTGCTCAG CCTGTTCAGA CAAAGTTCCC TGCCTATGAA
    AGAGTTGTTC TTCGGGAAGC TGGGTTTCTC AGACCTGTGA CCATTTTTGG TCCAATAGCT
    GATGTTGCAC GAGAGAAACT AGCCAGAGAA GAACCAGATA TCTTTCAAAT TGCAAAAAGT
    GAACCGAGAG ATGCTGGTAC TGACCAACGT AGTTCTGGCA TTATTCGTCT TCATACGATA
    AAACAGATAA TTGATAGAGA CAAACACGCT TTATTAGATG TCACTCCTAA TGCAGTGGAC
    CGTCTGAATT ATGCACAGTG GTATCCTATT GTAGTGTTTC TGAACCCTGA TTCTAAGCAG
    GGTGTGAAGA CCATGAGAAT GAGGCTGTGC CCAGAATCAC GAAAAAGTGC CAGGAAGCTA
    TATGAAAGAG CTCACAAGCT ACGCAAAAAT AATCACCACC TCTTTACAAC TACCATTAAC
    TTGAATTCAA TGAATGAAGG ATGGTATGGA GCTCTAAAAG AAGCAATTCA GCAACAACAA
    AACCAACTGG TGTGGGTTTC TGAAGGAAAG GCAGATGGTG CTACAAGTGA TGACCTTGAT
    TTACATGATG ACCGCCTGTC TTACCTCTCA GCTCCTGGTA GTGAATATTC TATGTACAGT
    ACAGACAGCA GACATACATC TGACTATGAA GACACAGATA CAGAAGGTGG TGCTTATACT
    GATCAAGAAC TGGATGAAAC TCTGAACGAT GAGGTTGGAA CTCCTCCTGA ATCTGCTATT
    ACACGTTCTT CTGAACCCGT TAGGGAGGAT TCTTCTGGAA TGCATCATGA AACTCAAACT
    TACACTAGTT ACGCATCTCA AGCTCAGCCT CAGCCAAATC TTAGAATAGA TTCTTCAGGA
    TTTAAAACAA CTGCTTCTCA GCCGGTATAC AGAAAGGATC CATATATTAG TGAAGAAGCA
    TCCAGACAAA GCTATGTCTT AAAACAGCCA GCTGTTAATC ACCCAGTACA AAGACAGGAA
    AGAGACCCAA ATCTGATCTA TGAATCTCAG ACTCAGTATG CAGAAAAACA ACCAAGTAGG
    GAGTACGAGC AGTCAACATA CAGGTATGAT TCTACAAACT ATGTAGATCA ATTTTCTCGT
    GGGTATGACC CTCGTCTACA TTATGAAGAT CGTGCGCCTC CCTATGAGGA GCACTGGTCA
    TATTATGATG AAAAACAGCC CTACAATCAA CCAAGAACAG CTTATGAAAG CCAGCCTCCT
    AGAGATCTTG ATTCCAGACA AAATACAGAA GAGAGCACAG AACGCAGCTA TTATCCGGCA
    CAACCCCGAT TTGAGGAACC TCCTCCGATG AGCTACGATG GCAGACCTCG CTATGAGCAT
    GCACCCAAAA ACTTCAGCTT GCCACAAGTG CGGTATGAAG ATCAACATCC TGTTGGCTAT
    GACACACATG CGCATGGTAG ATATAAACAA GAAGCTCAGC CATACCAGTC AGCCATATCT
    CGATCTCCTG AACCCAAGCA GTACTTTGAT CCACATGTGA GGGGCTATGA GCAAGGTCCT
    CCTCAAGCTT ACAATGCAAA AGCTGGACAG TTTGAGCCTT CTCATAGCAC TTCAGGTGTT
    TCTCTTCCTC CTCCCGCTTC TTCACAAACA AAACCAGAAG TTTTGCCTTC CAACAGTAAA
    CCACTGCCTA CACCACCATC TCTAGCAGAA GAAGAGGAGG ATCCAGCCAT GAAACCACAG
    TCTGTGCGAA GTAGAGTTAA GATATTTGAA CGAAGAAGAT CGCCATCTCT GGAAAAAATG
    AAGGACCCCA GTGACACGTC AGTTGTCAAA CCTCCAGAGC TAGCACCGAA GCCTACGCTC
    CCAGCCATGA GTGGTCCAAA ACCAACTTCT CAAAGCCAGT ATGAACATGA TAAAACAACT
    TACAGGGCCC CAGAACCACA GAGACCTCAA GTGAAGCCAC CTGAAGATAT CGTGCGTTCA
    AATCATTATG ATCCCGAAGA AGATGAAGAG TATTACAGAA AGCAGCTCTC ATACTTTGAT
    CGCAGGAATT TTGAAAATAA GCCTTCTGCA CAGGTTCCTG CCAGCCATCA TTCTGACTCC
    ACTAAACCAA TACATTCACA AAATCAGCTG AATTTCTCCA ATTATTCTAA GGGGAAGCCA
    ACTGATGCTG AACCAATGGA TAGATCTGTT GGTGACAAGC GCTATGAGCC AATCCCTCAA
    GTTACAACTC CTCCTCCTGC TCCTTCAGTA CAGTATACAC AACCTCAGTC AATTAATAGT
    CCTGTCCTGT CTCTCCCAGC GCATCACAAG CCTGCACTTT CTGAAGTTAA CTCTGTGTCT
    GACCCTCCTC CACCTCAGAA TAAGCCAGCA ATTTTCAGAT CCTCTAGAGA GGACACTGTG
    CAGTCTACTT TTTATCCTCA GAAAAGCTTC CCTGACAAAG GTCCTGTTAA TGGAACTGAA
    CAGGTTCAGA AAACAGTCAC TCCTTCTTAC AACCGTTTTA CAACAAAACC TTACACTAGT
    GCTGCGAGGC CCTTTGAGCG CAAGTTTGAA AGTCCTAAAT TCAACCATAA TCTCTTGCCA
    AATGAAGCTC AACATAAACC AGAGTTGCCA TCAAAATCTC CAAATTCTCC TCAACCAATT
    TTGAAAGCAC ACAGCTCATC ACAGCCTCCT GAGTTTGACA GTGGAATGGA CACCTTCACT
    GTACAGGTTG ACAAGCCTAA ATATCAACCA AATAATGTAA ATGCTGTGCC TAAAGCCATT
    CCTGTAAGCC CTTCAGCACT AGAGGATGAG GAGGAAGAAG ACGGACACAC TGTTGTAGCC
    ACAGCAAGAG GTGTATTCAA CAGCAATGGT GGCGTATTGA GCTCCATAGA GACTGGAGTT
    AGTATTATTA TACCACAAGG AGCCATTCCA GAGGGAATAG AGCAAGAAAT ATATTTTAAA
    GTCTGCAGAG ACAACAGTAT ACTTCCACCT TTGGACAAAG AGAAAGGTGA AACACTTCTT
    AGCCCCTTGG TAATGTGTGG GCCTCACGGA CTAAAATTCC TGAAGCCAGT GGAGCTGCGC
    CTGCCACACT GTGCGTCTAT GACCCCTGAT GGTTGGTCTT TTGCTCTAAA ATCCTCTGAC
    TCCTCGTCGG GTGACCCCAA AACCTGGCAG AACAAGTCTC TTCCTGGAGA TCCAAACTAT
    CTTGTTGGAG CAAACTGTGT CTCAGTCCTA ATTGACCACT TCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015134465.1
    CDS16..5280
    Translation

    Target ORF information:

    RefSeq Version XM_015278979.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus tight junction protein 1 (TJP1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015278979.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAAGTATC AGAAATACCT GACTGTCTTG CAGATGGCCA TAGGGGTGAC CGCCTCCAAC 
    AGGGGCAGTC TGATGCCCTT AAAGAGGAAG CTGTGGGTAA CTCCATCTTC CGAAAATCCC
    AATGGTGCTA CCTGTAGTGT AAGTCAGGGA AAGCCTTCTT TAAGAAGAAT AAAAGGGAGA
    ATACACAGAA GCAAAAGCCT TGATAGCATT GATTTCTGTG AATTCACTAG TGCAACAATG
    GAAGAAACAG CTATATGGGA ACAACACACG GTGACTCTTC ACAGGGCTCC TGGATTTGGG
    TTTGGTATTG CAATATCTGG AGGAAGGGAT AATCCTCACT TTCAAAGTGG TGAGACCTCA
    ATAGTTATTT CTGATGTGCT GAAAGGAGGC CCAGCAGAAG GACTGCTACA AGAAAATGAC
    CGTGTTGCAA TGGTTAATGG AGTTTCAATG GATAATGTTG AGCACGCATT TGCTGTTCAG
    CAGCTGAGGA AAAGTGGGAA GAATGCCAAA ATTACTATCC GAAGGATGAA GAAAATTCAG
    ATTCCAGTAG CTCGACAGGA ACCTGAGCCA GTGTCTGAAA ATGAGGACGA TAGCTATGAT
    GAAGAGATAC GGGATCCAAG GACCAGCCGT GGTGCTTCCA GTGCCAACAG AAGGCATGAG
    AAGAGCTGGG TACGAGACCG AAGTGCAAGC AGAGAAAGAA GCTTATCACC AAGATCTGAC
    AGAAGATCGG TTACTTCCAG TCAGCCTGCA AAACCTACCA AAGTAACGTT GGTGAAATCA
    AGGAAAAATG AAGAGTATGG TCTACGGTTG GCAAGCCACA TATTTGTGAA AGAAATATCA
    CAGGATAGCC TTGCAGCAAG AGATGGCAAT ATTCAGGAAG GGGATGTTGT TCTGAAGATA
    AATGGCACAG TGACAGAAAA TATGTCCTTA GCAGATGCAA AAACGCTAAT AGAAAGGTCC
    AAAGGCAAGT TGAAGATGGT GGTTCAGCGA GATGAACGAG CTACACTGTT GAATGTCCCT
    GATCTTTCTG ACAGTATTCA TTCTGCTAAT GCTTCGGAAA GAGATGACAT CTCAGAAATT
    CAGTCGCTGG CATCAGATCA TTCCAACCGA TCACATGACA GGCCCCGCCG CAGTCGTTCA
    CGATCTCCTG ACCAGAGGTC AGAGCCTTCA GACCATTCCA GACATTCTCC ACAGCAGCCC
    AGCAACGGCA GTCTTCGAAG CAGAGAAGAT GAAAGGGTAA CTAAACCAGG AGCCATCTCT
    ACACCTGTAA AGAATGCAGA TGATATTTCT AAAACTATGG AAGAGGTGTC AGTCGAAAGA
    ACTGAAAAAC AAACACCACC GCTCCCAGAA CCAAAGCCAG TATATGCACA AGGAGGTCAG
    CCAGATGTGG ATTTACCAGT CAGTCCGTCT GATGGTCCTT TACCCAATTC AACCCATGAA
    GATGGAATGC TTCGGCCAAG CATGAAACTG GTAAAATTCA GAAAAGGAGA TAGTGTGGGC
    CTGCGGCTCG CTGGTGGCAA TGATGTTGGC ATATTTGTAG CTGGTGTTCT AGAGGACAGC
    CCTGCAGCGA AAGAAGGATT AGAGGAAGGA GACCAAATTC TCAGGGTTAA TAACGTAGAC
    TTCACAAATA TCATCAGAGA AGAAGCTGTC CTTTTTTTGC TTGATCTCCC TAAAGGCGAA
    GAAGTAACCA TTTTGGCACA GAAGAAGAAA GATGTTTATC GTCGCATTGT TGAGTCTGAT
    GTAGGGGATT CTTTCTATAT CAGAACTCAT TTTGAATATG AAAAGGAATC TCCTTATGGA
    CTAAGCTTCA ACAAAGGTGA AGTGTTTCGG GTTGTGGACA CGCTATATAA TGGCAAACTG
    GGTTCGTGGC TGGCAATTCG AATTGGCAAG AATCATAAGG AAGTTGAAAG AGGTATCATA
    CCTAACAAAA ACAGAGCTGA ACAACTAGCT AGTGTACAGT ACACGCTTCC AAAGACAGCA
    GGAGGAGATC GAGCGGATTT CTGGAGATTC CGAGGTTTGC GTAGCTCAAA AAGAAATCTC
    CGAAAAAGTA GAGAAGATCT TTCTGCTCAG CCTGTTCAGA CAAAGTTCCC TGCCTATGAA
    AGAGTTGTTC TTCGGGAAGC TGGGTTTCTC AGACCTGTGA CCATTTTTGG TCCAATAGCT
    GATGTTGCAC GAGAGAAACT AGCCAGAGAA GAACCAGATA TCTTTCAAAT TGCAAAAAGT
    GAACCGAGAG ATGCTGGTAC TGACCAACGT AGTTCTGGCA TTATTCGTCT TCATACGATA
    AAACAGATAA TTGATAGAGA CAAACACGCT TTATTAGATG TCACTCCTAA TGCAGTGGAC
    CGTCTGAATT ATGCACAGTG GTATCCTATT GTAGTGTTTC TGAACCCTGA TTCTAAGCAG
    GGTGTGAAGA CCATGAGAAT GAGGCTGTGC CCAGAATCAC GAAAAAGTGC CAGGAAGCTA
    TATGAAAGAG CTCACAAGCT ACGCAAAAAT AATCACCACC TCTTTACAAC TACCATTAAC
    TTGAATTCAA TGAATGAAGG ATGGTATGGA GCTCTAAAAG AAGCAATTCA GCAACAACAA
    AACCAACTGG TGTGGGTTTC TGAAGGAAAG GCAGATGGTG CTACAAGTGA TGACCTTGAT
    TTACATGATG ACCGCCTGTC TTACCTCTCA GCTCCTGGTA GTGAATATTC TATGTACAGT
    ACAGACAGCA GACATACATC TGACTATGAA GACACAGATA CAGAAGGTGG TGCTTATACT
    GATCAAGAAC TGGATGAAAC TCTGAACGAT GAGGTTGGAA CTCCTCCTGA ATCTGCTATT
    ACACGTTCTT CTGAACCCGT TAGGGAGGAT TCTTCTGGAA TGCATCATGA AACTCAAACT
    TACACTAGTT ACGCATCTCA AGCTCAGCCT CAGCCAAATC TTAGAATAGA TTCTTCAGGA
    TTTAAAACAA CTGCTTCTCA GCCGGTATAC AGAAAGGATC CATATATTAG TGAAGAAGCA
    TCCAGACAAA GCTATGTCTT AAAACAGCCA GCTGTTAATC ACCCAGTACA AAGACAGGAA
    AGAGACCCAA ATCTGATCTA TGAATCTCAG ACTCAGTATG CAGAAAAACA ACCAAGTAGG
    GAGTACGAGC AGTCAACATA CAGGTATGAT TCTACAAACT ATGTAGATCA ATTTTCTCGT
    GGGTATGACC CTCGTCTACA TTATGAAGAT CGTGCGCCTC CCTATGAGGA GCACTGGTCA
    TATTATGATG AAAAACAGCC CTACAATCAA CCAAGAACAG CTTATGAAAG CCAGCCTCCT
    AGAGATCTTG ATTCCAGACA AAATACAGAA GAGAGCACAG AACGCAGCTA TTATCCGGCA
    CAACCCCGAT TTGAGGAACC TCCTCCGATG AGCTACGATG GCAGACCTCG CTATGAGCAT
    GCACCCAAAA ACTTCAGCTT GCCACAAGTG CGGTATGAAG ATCAACATCC TGTTGGCTAT
    GACACACATG CGCATGGTAG ATATAAACAA GAAGCTCAGC CATACCAGTC AGCCATATCT
    CGATCTCCTG AACCCAAGCA GTACTTTGAT CCACATGTGA GGGGCTATGA GCAAGGTCCT
    CCTCAAGCTT ACAATGCAAA AGCTGGACAG TTTGAGCCTT CTCATAGCAC TTCAGGTGTT
    TCTCTTCCTC CTCCCGCTTC TTCACAAACA AAACCAGAAG TTTTGCCTTC CAACAGTAAA
    CCACTGCCTA CACCACCATC TCTAGCAGAA GAAGAGGAGG ATCCAGCCAT GAAACCACAG
    TCTGTGCGAA GTAGAGTTAA GATATTTGAA CGAAGAAGAT CGCCATCTCT GGAAAAAATG
    AAGGACCCCA GTGACACGTC AGTTGTCAAA CCTCCAGAGC TAGCACCGAA GCCTACGCTC
    CCAGCCATGA GTGGTCCAAA ACCAACTTCT CAAAGCCAGT ATGAACATGA TAAAACAACT
    TACAGGGCCC CAGAACCACA GAGACCTCAA GTGAAGCCAC CTGAAGATAT CGTGCGTTCA
    AATCATTATG ATCCCGAAGA AGATGAAGAG TATTACAGAA AGCAGCTCTC ATACTTTGAT
    CGCAGGAATT TTGAAAATAA GCCTTCTGCA CAGGTTCCTG CCAGCCATCA TTCTGACTCC
    ACTAAACCAA TACATTCACA AAATCAGCTG AATTTCTCCA ATTATTCTAA GGGGAAGCCA
    ACTGATGCTG AACCAATGGA TAGATCTGTT GGTGACAAGC GCTATGAGCC AATCCCTCAA
    GTTACAACTC CTCCTCCTGC TCCTTCAGTA CAGTATACAC AACCTCAGTC AATTAATAGT
    CCTGTCCTGT CTCTCCCAGC GCATCACAAG CCTGCACTTT CTGAAGTTAA CTCTGTGTCT
    GACCCTCCTC CACCTCAGAA TAAGCCAGCA ATTTTCAGAT CCTCTAGAGA GGACACTGTG
    CAGTCTACTT TTTATCCTCA GAAAAGCTTC CCTGACAAAG GTCCTGTTAA TGGAACTGAA
    CAGGTTCAGA AAACAGTCAC TCCTTCTTAC AACCGTTTTA CAACAAAACC TTACACTAGT
    GCTGCGAGGC CCTTTGAGCG CAAGTTTGAA AGTCCTAAAT TCAACCATAA TCTCTTGCCA
    AATGAAGCTC AACATAAACC AGAGTTGCCA TCAAAATCTC CAAATTCTCC TCAACCAATT
    TTGAAAGCAC ACAGCTCATC ACAGCCTCCT GAGTTTGACA GTGGAATGGA CACCTTCACT
    GTACAGGTTG ACAAGCCTAA ATATCAACCA AATAATGTAA ATGCTGTGCC TAAAGCCATT
    CCTGTAAGCC CTTCAGCACT AGAGGATGAG GAGGAAGAAG ACGGACACAC TGTTGTAGCC
    ACAGCAAGAG GTGTATTCAA CAGCAATGGT GGCGTATTGA GCTCCATAGA GACTGGAGTT
    AGTATTATTA TACCACAAGG AGCCATTCCA GAGGGAATAG AGCAAGAAAT ATATTTTAAA
    GTCTGCAGAG ACAACAGTAT ACTTCCACCT TTGGACAAAG AGAAAGGTGA AACACTTCTT
    AGCCCCTTGG TAATGTGTGG GCCTCACGGA CTAAAATTCC TGAAGCCAGT GGAGCTGCGC
    CTGCCACACT GTGCGTCTAT GACCCCTGAT GGTTGGTCTT TTGCTCTAAA ATCCTCTGAC
    TCCTCGTCGG GTGACCCCAA AACCTGGCAG AACAAGTCTC TTCCTGGAGA TCCAAACTAT
    CTTGTTGGAG CAAACTGTGT CTCAGTCCTA ATTGACCACT TCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa40355
    Clone ID Related Accession (Same CDS sequence) XM_015278980.1 , XM_015278980.2
    Accession Version XM_015278980.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5316bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product tight junction protein ZO-1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763853.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCTGCCA GGGCCGCGTC CGGCAAGAGT GCAACAATGG AAGAAACAGC TATATGGGAA 
    CAACACACGG TGACTCTTCA CAGGGCTCCT GGATTTGGGT TTGGTATTGC AATATCTGGA
    GGAAGGGATA ATCCTCACTT TCAAAGTGGT GAGACCTCAA TAGTTATTTC TGATGTGCTG
    AAAGGAGGCC CAGCAGAAGG ACTGCTACAA GAAAATGACC GTGTTGCAAT GGTTAATGGA
    GTTTCAATGG ATAATGTTGA GCACGCATTT GCTGTTCAGC AGCTGAGGAA AAGTGGGAAG
    AATGCCAAAA TTACTATCCG AAGGATGAAG AAAATTCAGA TTCCAGTAGC TCGACAGGAA
    CCTGAGCCAG TGTCTGAAAA TGAGGACGAT AGCTATGATG AAGAGATACG GGATCCAAGG
    ACCAGCCGTG GTGCTTCCAG TGCCAACAGA AGGCATGAGA AGAGCTGGGT ACGAGACCGA
    AGTGCAAGCA GAGAAAGAAG CTTATCACCA AGATCTGACA GAAGATCGGT TACTTCCAGT
    CAGCCTGCAA AACCTACCAA AGTAACGTTG GTGAAATCAA GGAAAAATGA AGAGTATGGT
    CTACGGTTGG CAAGCCACAT ATTTGTGAAA GAAATATCAC AGGATAGCCT TGCAGCAAGA
    GATGGCAATA TTCAGGAAGG GGATGTTGTT CTGAAGATAA ATGGCACAGT GACAGAAAAT
    ATGTCCTTAG CAGATGCAAA AACGCTAATA GAAAGGTCCA AAGGCAAGTT GAAGATGGTG
    GTTCAGCGAG ATGAACGAGC TACACTGTTG AATGTCCCTG ATCTTTCTGA CAGTATTCAT
    TCTGCTAATG CTTCGGAAAG AGATGACATC TCAGAAATTC AGTCGCTGGC ATCAGATCAT
    TCCAACCGAT CACATGACAG GCCCCGCCGC AGTCGTTCAC GATCTCCTGA CCAGAGGTCA
    GAGCCTTCAG ACCATTCCAG ACATTCTCCA CAGCAGCCCA GCAACGGCAG TCTTCGAAGC
    AGAGAAGATG AAAGGGTAAC TAAACCAGGA GCCATCTCTA CACCTGTAAA GAATGCAGAT
    GATATTTCTA AAACTATGGA AGAGGTGTCA GTCGAAAGAA CTGAAAAACA AACACCACCG
    CTCCCAGAAC CAAAGCCAGT ATATGCACAA GGAGGTCAGC CAGATGTGGA TTTACCAGTC
    AGTCCGTCTG ATGGTCCTTT ACCCAATTCA ACCCATGAAG ATGGAATGCT TCGGCCAAGC
    ATGAAACTGG TAAAATTCAG AAAAGGAGAT AGTGTGGGCC TGCGGCTCGC TGGTGGCAAT
    GATGTTGGCA TATTTGTAGC TGGTGTTCTA GAGGACAGCC CTGCAGCGAA AGAAGGATTA
    GAGGAAGGAG ACCAAATTCT CAGGGTTAAT AACGTAGACT TCACAAATAT CATCAGAGAA
    GAAGCTGTCC TTTTTTTGCT TGATCTCCCT AAAGGCGAAG AAGTAACCAT TTTGGCACAG
    AAGAAGAAAG ATGTTTATCG TCGCATTGTT GAGTCTGATG TAGGGGATTC TTTCTATATC
    AGAACTCATT TTGAATATGA AAAGGAATCT CCTTATGGAC TAAGCTTCAA CAAAGGTGAA
    GTGTTTCGGG TTGTGGACAC GCTATATAAT GGCAAACTGG GTTCGTGGCT GGCAATTCGA
    ATTGGCAAGA ATCATAAGGA AGTTGAAAGA GGTATCATAC CTAACAAAAA CAGAGCTGAA
    CAACTAGCTA GTGTACAGTA CACGCTTCCA AAGACAGCAG GAGGAGATCG AGCGGATTTC
    TGGAGATTCC GAGGTTTGCG TAGCTCAAAA AGAAATCTCC GAAAAAGTAG AGAAGATCTT
    TCTGCTCAGC CTGTTCAGAC AAAGTTCCCT GCCTATGAAA GAGTTGTTCT TCGGGAAGCT
    GGGTTTCTCA GACCTGTGAC CATTTTTGGT CCAATAGCTG ATGTTGCACG AGAGAAACTA
    GCCAGAGAAG AACCAGATAT CTTTCAAATT GCAAAAAGTG AACCGAGAGA TGCTGGTACT
    GACCAACGTA GTTCTGGCAT TATTCGTCTT CATACGATAA AACAGATAAT TGATAGAGAC
    AAACACGCTT TATTAGATGT CACTCCTAAT GCAGTGGACC GTCTGAATTA TGCACAGTGG
    TATCCTATTG TAGTGTTTCT GAACCCTGAT TCTAAGCAGG GTGTGAAGAC CATGAGAATG
    AGGCTGTGCC CAGAATCACG AAAAAGTGCC AGGAAGCTAT ATGAAAGAGC TCACAAGCTA
    CGCAAAAATA ATCACCACCT CTTTACAACT ACCATTAACT TGAATTCAAT GAATGAAGGA
    TGGTATGGAG CTCTAAAAGA AGCAATTCAG CAACAACAAA ACCAACTGGT GTGGGTTTCT
    GAAGGAAAGG CAGATGGTGC TACAAGTGAT GACCTTGATT TACATGATGA CCGCCTGTCT
    TACCTCTCAG CTCCTGGTAG TGAATATTCT ATGTACAGTA CAGACAGCAG ACATACATCT
    GACTATGAAG ACACAGATAC AGAAGGTGGT GCTTATACTG ATCAAGAACT GGATGAAACT
    CTGAACGATG AGGTTGGAAC TCCTCCTGAA TCTGCTATTA CACGTTCTTC TGAACCCGTT
    AGGGAGGATT CTTCTGGAAT GCATCATGAA ACTCAAACTT ACACTAGTTA CGCATCTCAA
    GCTCAGCCTC AGCCAAATCT TAGAATAGAT TCTTCAGGAT TTAAAACAAC TGCTTCTCAG
    CCGAAAGCAG AAGCATCACC TGCAGTCCCT TACCTTTCCC CGTCGCCTGA ATCAAACCCA
    GCAACCTCAT CAACCTCTGC AGTAAATGCT AATGTAAACC TAACTAATGT CAGACTAGAG
    GAGCCTACTG CTGCTCCTTA CAACTCTTAT CAACCACAAG CGGGCCCCTT AAGAACATCA
    AGTACTGAGG GAGCTCATGT AGTGCTAAGA GATCAAGAGC CATCATCCTT ACCGCCGCAT
    ATAGATCCAG CAAAGGTATA CAGAAAGGAT CCATATATTA GTGAAGAAGC ATCCAGACAA
    AGCTATGTCT TAAAACAGCC AGCTGTTAAT CACCCAGTAC AAAGACAGGA AAGAGACCCA
    AATCTGATCT ATGAATCTCA GACTCAGTAT GCAGAAAAAC AACCAAGTAG GGAGTACGAG
    CAGTCAACAT ACAGGTATGA TTCTACAAAC TATGTAGATC AATTTTCTCG TGGGTATGAC
    CCTCGTCTAC ATTATGAAGA TCGTGCGCCT CCCTATGAGG AGCACTGGTC ATATTATGAT
    GAAAAACAGC CCTACAATCA ACCAAGAACA GCTTATGAAA GCCAGCCTCC TAGAGATCTT
    GATTCCAGAC AAAATACAGA AGAGAGCACA GAACGCAGCT ATTATCCGGC ACAACCCCGA
    TTTGAGGAAC CTCCTCCGAT GAGCTACGAT GGCAGACCTC GCTATGAGCA TGCACCCAAA
    AACTTCAGCT TGCCACAAGT GCGGTATGAA GATCAACATC CTGTTGGCTA TGACACACAT
    GCGCATGGTA GATATAAACA AGAAGCTCAG CCATACCAGT CAGCCATATC TCGATCTCCT
    GAACCCAAGC AGTACTTTGA TCCACATGTG AGGGGCTATG AGCAAGGTCC TCCTCAAGCT
    TACAATGCAA AAGCTGGACA GTTTGAGCCT TCTCATAGCA CTTCAGGTGT TTCTCTTCCT
    CCTCCCGCTT CTTCACAAAC AAAACCAGAA GTTTTGCCTT CCAACAGTAA ACCACTGCCT
    ACACCACCAT CTCTAGCAGA AGAAGAGGAG GATCCAGCCA TGAAACCACA GTCTGTGCGA
    AGTAGAGTTA AGATATTTGA ACGAAGAAGA TCGCCATCTC TGGAAAAAAT GAAGGACCCC
    AGTGACACGT CAGTTGTCAA ACCTCCAGAG CTAGCACCGA AGCCTACGCT CCCAGCCATG
    AGTGGTCCAA AACCAACTTC TCAAAGCCAG TATGAACATG ATAAAACAAC TTACAGGGCC
    CCAGAACCAC AGAGACCTCA AGTGAAGCCA CCTGAAGATA TCGTGCGTTC AAATCATTAT
    GATCCCGAAG AAGATGAAGA GTATTACAGA AAGCAGCTCT CATACTTTGA TCGCAGGAAT
    TTTGAAAATA AGCCTTCTGC ACAGGTTCCT GCCAGCCATC ATTCTGACTC CACTAAACCA
    ATACATTCAC AAAATCAGCT GAATTTCTCC AATTATTCTA AGGGGAAGCC AACTGATGCT
    GAACCAATGG ATAGATCTGT TGGTGACAAG CGCTATGAGC CAATCCCTCA AGTTACAACT
    CCTCCTCCTG CTCCTTCAGT ACAGTATACA CAACCTCAGT CAATTAATAG TCCTGTCCTG
    TCTCTCCCAG CGCATCACAA GCCTGCACTT TCTGAAGTTA ACTCTGTGTC TGACCCTCCT
    CCACCTCAGA ATAAGCCAGC AATTTTCAGA TCCTCTAGAG AGGACACTGT GCAGTCTACT
    TTTTATCCTC AGAAAAGCTT CCCTGACAAA GGTCCTGTTA ATGGAACTGA ACAGGTTCAG
    AAAACAGTCA CTCCTTCTTA CAACCGTTTT ACAACAAAAC CTTACACTAG TGCTGCGAGG
    CCCTTTGAGC GCAAGTTTGA AAGTCCTAAA TTCAACCATA ATCTCTTGCC AAATGAAGCT
    CAACATAAAC CAGAGTTGCC ATCAAAATCT CCAAATTCTC CTCAACCAAT TTTGAAAGCA
    CACAGCTCAT CACAGCCTCC TGAGTTTGAC AGTGGAATGG ACACCTTCAC TGTACAGGTT
    GACAAGCCTA AATATCAACC AAATAATGTA AATGCTGTGC CTAAAGCCAT TCCTGTAAGC
    CCTTCAGCAC TAGAGGATGA GGAGGAAGAA GACGGACACA CTGTTGTAGC CACAGCAAGA
    GGTGTATTCA ACAGCAATGG TGGCGTATTG AGCTCCATAG AGACTGGAGT TAGTATTATT
    ATACCACAAG GAGCCATTCC AGAGGGAATA GAGCAAGAAA TATATTTTAA AGTCTGCAGA
    GACAACAGTA TACTTCCACC TTTGGACAAA GAGAAAGGTG AAACACTTCT TAGCCCCTTG
    GTAATGTGTG GGCCTCACGG ACTAAAATTC CTGAAGCCAG TGGAGCTGCG CCTGCCACAC
    TGTGCGTCTA TGACCCCTGA TGGTTGGTCT TTTGCTCTAA AATCCTCTGA CTCCTCGTCG
    GGTGACCCCA AAACCTGGCA GAACAAGTCT CTTCCTGGAG ATCCAAACTA TCTTGTTGGA
    GCAAACTGTG TCTCAGTCCT AATTGACCAC TTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015134466.1
    CDS299..5614
    Translation

    Target ORF information:

    RefSeq Version XM_015278980.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus tight junction protein 1 (TJP1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015278980.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCTGCCA GGGCCGCGTC CGGCAAGAGT GCAACAATGG AAGAAACAGC TATATGGGAA 
    CAACACACGG TGACTCTTCA CAGGGCTCCT GGATTTGGGT TTGGTATTGC AATATCTGGA
    GGAAGGGATA ATCCTCACTT TCAAAGTGGT GAGACCTCAA TAGTTATTTC TGATGTGCTG
    AAAGGAGGCC CAGCAGAAGG ACTGCTACAA GAAAATGACC GTGTTGCAAT GGTTAATGGA
    GTTTCAATGG ATAATGTTGA GCACGCATTT GCTGTTCAGC AGCTGAGGAA AAGTGGGAAG
    AATGCCAAAA TTACTATCCG AAGGATGAAG AAAATTCAGA TTCCAGTAGC TCGACAGGAA
    CCTGAGCCAG TGTCTGAAAA TGAGGACGAT AGCTATGATG AAGAGATACG GGATCCAAGG
    ACCAGCCGTG GTGCTTCCAG TGCCAACAGA AGGCATGAGA AGAGCTGGGT ACGAGACCGA
    AGTGCAAGCA GAGAAAGAAG CTTATCACCA AGATCTGACA GAAGATCGGT TACTTCCAGT
    CAGCCTGCAA AACCTACCAA AGTAACGTTG GTGAAATCAA GGAAAAATGA AGAGTATGGT
    CTACGGTTGG CAAGCCACAT ATTTGTGAAA GAAATATCAC AGGATAGCCT TGCAGCAAGA
    GATGGCAATA TTCAGGAAGG GGATGTTGTT CTGAAGATAA ATGGCACAGT GACAGAAAAT
    ATGTCCTTAG CAGATGCAAA AACGCTAATA GAAAGGTCCA AAGGCAAGTT GAAGATGGTG
    GTTCAGCGAG ATGAACGAGC TACACTGTTG AATGTCCCTG ATCTTTCTGA CAGTATTCAT
    TCTGCTAATG CTTCGGAAAG AGATGACATC TCAGAAATTC AGTCGCTGGC ATCAGATCAT
    TCCAACCGAT CACATGACAG GCCCCGCCGC AGTCGTTCAC GATCTCCTGA CCAGAGGTCA
    GAGCCTTCAG ACCATTCCAG ACATTCTCCA CAGCAGCCCA GCAACGGCAG TCTTCGAAGC
    AGAGAAGATG AAAGGGTAAC TAAACCAGGA GCCATCTCTA CACCTGTAAA GAATGCAGAT
    GATATTTCTA AAACTATGGA AGAGGTGTCA GTCGAAAGAA CTGAAAAACA AACACCACCG
    CTCCCAGAAC CAAAGCCAGT ATATGCACAA GGAGGTCAGC CAGATGTGGA TTTACCAGTC
    AGTCCGTCTG ATGGTCCTTT ACCCAATTCA ACCCATGAAG ATGGAATGCT TCGGCCAAGC
    ATGAAACTGG TAAAATTCAG AAAAGGAGAT AGTGTGGGCC TGCGGCTCGC TGGTGGCAAT
    GATGTTGGCA TATTTGTAGC TGGTGTTCTA GAGGACAGCC CTGCAGCGAA AGAAGGATTA
    GAGGAAGGAG ACCAAATTCT CAGGGTTAAT AACGTAGACT TCACAAATAT CATCAGAGAA
    GAAGCTGTCC TTTTTTTGCT TGATCTCCCT AAAGGCGAAG AAGTAACCAT TTTGGCACAG
    AAGAAGAAAG ATGTTTATCG TCGCATTGTT GAGTCTGATG TAGGGGATTC TTTCTATATC
    AGAACTCATT TTGAATATGA AAAGGAATCT CCTTATGGAC TAAGCTTCAA CAAAGGTGAA
    GTGTTTCGGG TTGTGGACAC GCTATATAAT GGCAAACTGG GTTCGTGGCT GGCAATTCGA
    ATTGGCAAGA ATCATAAGGA AGTTGAAAGA GGTATCATAC CTAACAAAAA CAGAGCTGAA
    CAACTAGCTA GTGTACAGTA CACGCTTCCA AAGACAGCAG GAGGAGATCG AGCGGATTTC
    TGGAGATTCC GAGGTTTGCG TAGCTCAAAA AGAAATCTCC GAAAAAGTAG AGAAGATCTT
    TCTGCTCAGC CTGTTCAGAC AAAGTTCCCT GCCTATGAAA GAGTTGTTCT TCGGGAAGCT
    GGGTTTCTCA GACCTGTGAC CATTTTTGGT CCAATAGCTG ATGTTGCACG AGAGAAACTA
    GCCAGAGAAG AACCAGATAT CTTTCAAATT GCAAAAAGTG AACCGAGAGA TGCTGGTACT
    GACCAACGTA GTTCTGGCAT TATTCGTCTT CATACGATAA AACAGATAAT TGATAGAGAC
    AAACACGCTT TATTAGATGT CACTCCTAAT GCAGTGGACC GTCTGAATTA TGCACAGTGG
    TATCCTATTG TAGTGTTTCT GAACCCTGAT TCTAAGCAGG GTGTGAAGAC CATGAGAATG
    AGGCTGTGCC CAGAATCACG AAAAAGTGCC AGGAAGCTAT ATGAAAGAGC TCACAAGCTA
    CGCAAAAATA ATCACCACCT CTTTACAACT ACCATTAACT TGAATTCAAT GAATGAAGGA
    TGGTATGGAG CTCTAAAAGA AGCAATTCAG CAACAACAAA ACCAACTGGT GTGGGTTTCT
    GAAGGAAAGG CAGATGGTGC TACAAGTGAT GACCTTGATT TACATGATGA CCGCCTGTCT
    TACCTCTCAG CTCCTGGTAG TGAATATTCT ATGTACAGTA CAGACAGCAG ACATACATCT
    GACTATGAAG ACACAGATAC AGAAGGTGGT GCTTATACTG ATCAAGAACT GGATGAAACT
    CTGAACGATG AGGTTGGAAC TCCTCCTGAA TCTGCTATTA CACGTTCTTC TGAACCCGTT
    AGGGAGGATT CTTCTGGAAT GCATCATGAA ACTCAAACTT ACACTAGTTA CGCATCTCAA
    GCTCAGCCTC AGCCAAATCT TAGAATAGAT TCTTCAGGAT TTAAAACAAC TGCTTCTCAG
    CCGAAAGCAG AAGCATCACC TGCAGTCCCT TACCTTTCCC CGTCGCCTGA ATCAAACCCA
    GCAACCTCAT CAACCTCTGC AGTAAATGCT AATGTAAACC TAACTAATGT CAGACTAGAG
    GAGCCTACTG CTGCTCCTTA CAACTCTTAT CAACCACAAG CGGGCCCCTT AAGAACATCA
    AGTACTGAGG GAGCTCATGT AGTGCTAAGA GATCAAGAGC CATCATCCTT ACCGCCGCAT
    ATAGATCCAG CAAAGGTATA CAGAAAGGAT CCATATATTA GTGAAGAAGC ATCCAGACAA
    AGCTATGTCT TAAAACAGCC AGCTGTTAAT CACCCAGTAC AAAGACAGGA AAGAGACCCA
    AATCTGATCT ATGAATCTCA GACTCAGTAT GCAGAAAAAC AACCAAGTAG GGAGTACGAG
    CAGTCAACAT ACAGGTATGA TTCTACAAAC TATGTAGATC AATTTTCTCG TGGGTATGAC
    CCTCGTCTAC ATTATGAAGA TCGTGCGCCT CCCTATGAGG AGCACTGGTC ATATTATGAT
    GAAAAACAGC CCTACAATCA ACCAAGAACA GCTTATGAAA GCCAGCCTCC TAGAGATCTT
    GATTCCAGAC AAAATACAGA AGAGAGCACA GAACGCAGCT ATTATCCGGC ACAACCCCGA
    TTTGAGGAAC CTCCTCCGAT GAGCTACGAT GGCAGACCTC GCTATGAGCA TGCACCCAAA
    AACTTCAGCT TGCCACAAGT GCGGTATGAA GATCAACATC CTGTTGGCTA TGACACACAT
    GCGCATGGTA GATATAAACA AGAAGCTCAG CCATACCAGT CAGCCATATC TCGATCTCCT
    GAACCCAAGC AGTACTTTGA TCCACATGTG AGGGGCTATG AGCAAGGTCC TCCTCAAGCT
    TACAATGCAA AAGCTGGACA GTTTGAGCCT TCTCATAGCA CTTCAGGTGT TTCTCTTCCT
    CCTCCCGCTT CTTCACAAAC AAAACCAGAA GTTTTGCCTT CCAACAGTAA ACCACTGCCT
    ACACCACCAT CTCTAGCAGA AGAAGAGGAG GATCCAGCCA TGAAACCACA GTCTGTGCGA
    AGTAGAGTTA AGATATTTGA ACGAAGAAGA TCGCCATCTC TGGAAAAAAT GAAGGACCCC
    AGTGACACGT CAGTTGTCAA ACCTCCAGAG CTAGCACCGA AGCCTACGCT CCCAGCCATG
    AGTGGTCCAA AACCAACTTC TCAAAGCCAG TATGAACATG ATAAAACAAC TTACAGGGCC
    CCAGAACCAC AGAGACCTCA AGTGAAGCCA CCTGAAGATA TCGTGCGTTC AAATCATTAT
    GATCCCGAAG AAGATGAAGA GTATTACAGA AAGCAGCTCT CATACTTTGA TCGCAGGAAT
    TTTGAAAATA AGCCTTCTGC ACAGGTTCCT GCCAGCCATC ATTCTGACTC CACTAAACCA
    ATACATTCAC AAAATCAGCT GAATTTCTCC AATTATTCTA AGGGGAAGCC AACTGATGCT
    GAACCAATGG ATAGATCTGT TGGTGACAAG CGCTATGAGC CAATCCCTCA AGTTACAACT
    CCTCCTCCTG CTCCTTCAGT ACAGTATACA CAACCTCAGT CAATTAATAG TCCTGTCCTG
    TCTCTCCCAG CGCATCACAA GCCTGCACTT TCTGAAGTTA ACTCTGTGTC TGACCCTCCT
    CCACCTCAGA ATAAGCCAGC AATTTTCAGA TCCTCTAGAG AGGACACTGT GCAGTCTACT
    TTTTATCCTC AGAAAAGCTT CCCTGACAAA GGTCCTGTTA ATGGAACTGA ACAGGTTCAG
    AAAACAGTCA CTCCTTCTTA CAACCGTTTT ACAACAAAAC CTTACACTAG TGCTGCGAGG
    CCCTTTGAGC GCAAGTTTGA AAGTCCTAAA TTCAACCATA ATCTCTTGCC AAATGAAGCT
    CAACATAAAC CAGAGTTGCC ATCAAAATCT CCAAATTCTC CTCAACCAAT TTTGAAAGCA
    CACAGCTCAT CACAGCCTCC TGAGTTTGAC AGTGGAATGG ACACCTTCAC TGTACAGGTT
    GACAAGCCTA AATATCAACC AAATAATGTA AATGCTGTGC CTAAAGCCAT TCCTGTAAGC
    CCTTCAGCAC TAGAGGATGA GGAGGAAGAA GACGGACACA CTGTTGTAGC CACAGCAAGA
    GGTGTATTCA ACAGCAATGG TGGCGTATTG AGCTCCATAG AGACTGGAGT TAGTATTATT
    ATACCACAAG GAGCCATTCC AGAGGGAATA GAGCAAGAAA TATATTTTAA AGTCTGCAGA
    GACAACAGTA TACTTCCACC TTTGGACAAA GAGAAAGGTG AAACACTTCT TAGCCCCTTG
    GTAATGTGTG GGCCTCACGG ACTAAAATTC CTGAAGCCAG TGGAGCTGCG CCTGCCACAC
    TGTGCGTCTA TGACCCCTGA TGGTTGGTCT TTTGCTCTAA AATCCTCTGA CTCCTCGTCG
    GGTGACCCCA AAACCTGGCA GAACAAGTCT CTTCCTGGAG ATCCAAACTA TCTTGTTGGA
    GCAAACTGTG TCTCAGTCCT AATTGACCAC TTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa40355
    Clone ID Related Accession (Same CDS sequence) XM_015278980.1 , XM_015278980.2
    Accession Version XM_015278980.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5316bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product tight junction protein ZO-1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006097.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015278980.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCTGCCA GGGCCGCGTC CGGCAAGAGT GCAACAATGG AAGAAACAGC TATATGGGAA 
    CAACACACGG TGACTCTTCA CAGGGCTCCT GGATTTGGGT TTGGTATTGC AATATCTGGA
    GGAAGGGATA ATCCTCACTT TCAAAGTGGT GAGACCTCAA TAGTTATTTC TGATGTGCTG
    AAAGGAGGCC CAGCAGAAGG ACTGCTACAA GAAAATGACC GTGTTGCAAT GGTTAATGGA
    GTTTCAATGG ATAATGTTGA GCACGCATTT GCTGTTCAGC AGCTGAGGAA AAGTGGGAAG
    AATGCCAAAA TTACTATCCG AAGGATGAAG AAAATTCAGA TTCCAGTAGC TCGACAGGAA
    CCTGAGCCAG TGTCTGAAAA TGAGGACGAT AGCTATGATG AAGAGATACG GGATCCAAGG
    ACCAGCCGTG GTGCTTCCAG TGCCAACAGA AGGCATGAGA AGAGCTGGGT ACGAGACCGA
    AGTGCAAGCA GAGAAAGAAG CTTATCACCA AGATCTGACA GAAGATCGGT TACTTCCAGT
    CAGCCTGCAA AACCTACCAA AGTAACGTTG GTGAAATCAA GGAAAAATGA AGAGTATGGT
    CTACGGTTGG CAAGCCACAT ATTTGTGAAA GAAATATCAC AGGATAGCCT TGCAGCAAGA
    GATGGCAATA TTCAGGAAGG GGATGTTGTT CTGAAGATAA ATGGCACAGT GACAGAAAAT
    ATGTCCTTAG CAGATGCAAA AACGCTAATA GAAAGGTCCA AAGGCAAGTT GAAGATGGTG
    GTTCAGCGAG ATGAACGAGC TACACTGTTG AATGTCCCTG ATCTTTCTGA CAGTATTCAT
    TCTGCTAATG CTTCGGAAAG AGATGACATC TCAGAAATTC AGTCGCTGGC ATCAGATCAT
    TCCAACCGAT CACATGACAG GCCCCGCCGC AGTCGTTCAC GATCTCCTGA CCAGAGGTCA
    GAGCCTTCAG ACCATTCCAG ACATTCTCCA CAGCAGCCCA GCAACGGCAG TCTTCGAAGC
    AGAGAAGATG AAAGGGTAAC TAAACCAGGA GCCATCTCTA CACCTGTAAA GAATGCAGAT
    GATATTTCTA AAACTATGGA AGAGGTGTCA GTCGAAAGAA CTGAAAAACA AACACCACCG
    CTCCCAGAAC CAAAGCCAGT ATATGCACAA GGAGGTCAGC CAGATGTGGA TTTACCAGTC
    AGTCCGTCTG ATGGTCCTTT ACCCAATTCA ACCCATGAAG ATGGAATGCT TCGGCCAAGC
    ATGAAACTGG TAAAATTCAG AAAAGGAGAT AGTGTGGGCC TGCGGCTCGC TGGTGGCAAT
    GATGTTGGCA TATTTGTAGC TGGTGTTCTA GAGGACAGCC CTGCAGCGAA AGAAGGATTA
    GAGGAAGGAG ACCAAATTCT CAGGGTTAAT AACGTAGACT TCACAAATAT CATCAGAGAA
    GAAGCTGTCC TTTTTTTGCT TGATCTCCCT AAAGGCGAAG AAGTAACCAT TTTGGCACAG
    AAGAAGAAAG ATGTTTATCG TCGCATTGTT GAGTCTGATG TAGGGGATTC TTTCTATATC
    AGAACTCATT TTGAATATGA AAAGGAATCT CCTTATGGAC TAAGCTTCAA CAAAGGTGAA
    GTGTTTCGGG TTGTGGACAC GCTATATAAT GGCAAACTGG GTTCGTGGCT GGCAATTCGA
    ATTGGCAAGA ATCATAAGGA AGTTGAAAGA GGTATCATAC CTAACAAAAA CAGAGCTGAA
    CAACTAGCTA GTGTACAGTA CACGCTTCCA AAGACAGCAG GAGGAGATCG AGCGGATTTC
    TGGAGATTCC GAGGTTTGCG TAGCTCAAAA AGAAATCTCC GAAAAAGTAG AGAAGATCTT
    TCTGCTCAGC CTGTTCAGAC AAAGTTCCCT GCCTATGAAA GAGTTGTTCT TCGGGAAGCT
    GGGTTTCTCA GACCTGTGAC CATTTTTGGT CCAATAGCTG ATGTTGCACG AGAGAAACTA
    GCCAGAGAAG AACCAGATAT CTTTCAAATT GCAAAAAGTG AACCGAGAGA TGCTGGTACT
    GACCAACGTA GTTCTGGCAT TATTCGTCTT CATACGATAA AACAGATAAT TGATAGAGAC
    AAACACGCTT TATTAGATGT CACTCCTAAT GCAGTGGACC GTCTGAATTA TGCACAGTGG
    TATCCTATTG TAGTGTTTCT GAACCCTGAT TCTAAGCAGG GTGTGAAGAC CATGAGAATG
    AGGCTGTGCC CAGAATCACG AAAAAGTGCC AGGAAGCTAT ATGAAAGAGC TCACAAGCTA
    CGCAAAAATA ATCACCACCT CTTTACAACT ACCATTAACT TGAATTCAAT GAATGAAGGA
    TGGTATGGAG CTCTAAAAGA AGCAATTCAG CAACAACAAA ACCAACTGGT GTGGGTTTCT
    GAAGGAAAGG CAGATGGTGC TACAAGTGAT GACCTTGATT TACATGATGA CCGCCTGTCT
    TACCTCTCAG CTCCTGGTAG TGAATATTCT ATGTACAGTA CAGACAGCAG ACATACATCT
    GACTATGAAG ACACAGATAC AGAAGGTGGT GCTTATACTG ATCAAGAACT GGATGAAACT
    CTGAACGATG AGGTTGGAAC TCCTCCTGAA TCTGCTATTA CACGTTCTTC TGAACCCGTT
    AGGGAGGATT CTTCTGGAAT GCATCATGAA ACTCAAACTT ACACTAGTTA CGCATCTCAA
    GCTCAGCCTC AGCCAAATCT TAGAATAGAT TCTTCAGGAT TTAAAACAAC TGCTTCTCAG
    CCGAAAGCAG AAGCATCACC TGCAGTCCCT TACCTTTCCC CGTCGCCTGA ATCAAACCCA
    GCAACCTCAT CAACCTCTGC AGTAAATGCT AATGTAAACC TAACTAATGT CAGACTAGAG
    GAGCCTACTG CTGCTCCTTA CAACTCTTAT CAACCACAAG CGGGCCCCTT AAGAACATCA
    AGTACTGAGG GAGCTCATGT AGTGCTAAGA GATCAAGAGC CATCATCCTT ACCGCCGCAT
    ATAGATCCAG CAAAGGTATA CAGAAAGGAT CCATATATTA GTGAAGAAGC ATCCAGACAA
    AGCTATGTCT TAAAACAGCC AGCTGTTAAT CACCCAGTAC AAAGACAGGA AAGAGACCCA
    AATCTGATCT ATGAATCTCA GACTCAGTAT GCAGAAAAAC AACCAAGTAG GGAGTACGAG
    CAGTCAACAT ACAGGTATGA TTCTACAAAC TATGTAGATC AATTTTCTCG TGGGTATGAC
    CCTCGTCTAC ATTATGAAGA TCGTGCGCCT CCCTATGAGG AGCACTGGTC ATATTATGAT
    GAAAAACAGC CCTACAATCA ACCAAGAACA GCTTATGAAA GCCAGCCTCC TAGAGATCTT
    GATTCCAGAC AAAATACAGA AGAGAGCACA GAACGCAGCT ATTATCCGGC ACAACCCCGA
    TTTGAGGAAC CTCCTCCGAT GAGCTACGAT GGCAGACCTC GCTATGAGCA TGCACCCAAA
    AACTTCAGCT TGCCACAAGT GCGGTATGAA GATCAACATC CTGTTGGCTA TGACACACAT
    GCGCATGGTA GATATAAACA AGAAGCTCAG CCATACCAGT CAGCCATATC TCGATCTCCT
    GAACCCAAGC AGTACTTTGA TCCACATGTG AGGGGCTATG AGCAAGGTCC TCCTCAAGCT
    TACAATGCAA AAGCTGGACA GTTTGAGCCT TCTCATAGCA CTTCAGGTGT TTCTCTTCCT
    CCTCCCGCTT CTTCACAAAC AAAACCAGAA GTTTTGCCTT CCAACAGTAA ACCACTGCCT
    ACACCACCAT CTCTAGCAGA AGAAGAGGAG GATCCAGCCA TGAAACCACA GTCTGTGCGA
    AGTAGAGTTA AGATATTTGA ACGAAGAAGA TCGCCATCTC TGGAAAAAAT GAAGGACCCC
    AGTGACACGT CAGTTGTCAA ACCTCCAGAG CTAGCACCGA AGCCTACGCT CCCAGCCATG
    AGTGGTCCAA AACCAACTTC TCAAAGCCAG TATGAACATG ATAAAACAAC TTACAGGGCC
    CCAGAACCAC AGAGACCTCA AGTGAAGCCA CCTGAAGATA TCGTGCGTTC AAATCATTAT
    GATCCCGAAG AAGATGAAGA GTATTACAGA AAGCAGCTCT CATACTTTGA TCGCAGGAAT
    TTTGAAAATA AGCCTTCTGC ACAGGTTCCT GCCAGCCATC ATTCTGACTC CACTAAACCA
    ATACATTCAC AAAATCAGCT GAATTTCTCC AATTATTCTA AGGGGAAGCC AACTGATGCT
    GAACCAATGG ATAGATCTGT TGGTGACAAG CGCTATGAGC CAATCCCTCA AGTTACAACT
    CCTCCTCCTG CTCCTTCAGT ACAGTATACA CAACCTCAGT CAATTAATAG TCCTGTCCTG
    TCTCTCCCAG CGCATCACAA GCCTGCACTT TCTGAAGTTA ACTCTGTGTC TGACCCTCCT
    CCACCTCAGA ATAAGCCAGC AATTTTCAGA TCCTCTAGAG AGGACACTGT GCAGTCTACT
    TTTTATCCTC AGAAAAGCTT CCCTGACAAA GGTCCTGTTA ATGGAACTGA ACAGGTTCAG
    AAAACAGTCA CTCCTTCTTA CAACCGTTTT ACAACAAAAC CTTACACTAG TGCTGCGAGG
    CCCTTTGAGC GCAAGTTTGA AAGTCCTAAA TTCAACCATA ATCTCTTGCC AAATGAAGCT
    CAACATAAAC CAGAGTTGCC ATCAAAATCT CCAAATTCTC CTCAACCAAT TTTGAAAGCA
    CACAGCTCAT CACAGCCTCC TGAGTTTGAC AGTGGAATGG ACACCTTCAC TGTACAGGTT
    GACAAGCCTA AATATCAACC AAATAATGTA AATGCTGTGC CTAAAGCCAT TCCTGTAAGC
    CCTTCAGCAC TAGAGGATGA GGAGGAAGAA GACGGACACA CTGTTGTAGC CACAGCAAGA
    GGTGTATTCA ACAGCAATGG TGGCGTATTG AGCTCCATAG AGACTGGAGT TAGTATTATT
    ATACCACAAG GAGCCATTCC AGAGGGAATA GAGCAAGAAA TATATTTTAA AGTCTGCAGA
    GACAACAGTA TACTTCCACC TTTGGACAAA GAGAAAGGTG AAACACTTCT TAGCCCCTTG
    GTAATGTGTG GGCCTCACGG ACTAAAATTC CTGAAGCCAG TGGAGCTGCG CCTGCCACAC
    TGTGCGTCTA TGACCCCTGA TGGTTGGTCT TTTGCTCTAA AATCCTCTGA CTCCTCGTCG
    GGTGACCCCA AAACCTGGCA GAACAAGTCT CTTCCTGGAG ATCCAAACTA TCTTGTTGGA
    GCAAACTGTG TCTCAGTCCT AATTGACCAC TTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015134466.1
    CDS298..5613
    Translation

    Target ORF information:

    RefSeq Version XM_015278980.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus tight junction protein 1 (TJP1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015278980.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCTGCCA GGGCCGCGTC CGGCAAGAGT GCAACAATGG AAGAAACAGC TATATGGGAA 
    CAACACACGG TGACTCTTCA CAGGGCTCCT GGATTTGGGT TTGGTATTGC AATATCTGGA
    GGAAGGGATA ATCCTCACTT TCAAAGTGGT GAGACCTCAA TAGTTATTTC TGATGTGCTG
    AAAGGAGGCC CAGCAGAAGG ACTGCTACAA GAAAATGACC GTGTTGCAAT GGTTAATGGA
    GTTTCAATGG ATAATGTTGA GCACGCATTT GCTGTTCAGC AGCTGAGGAA AAGTGGGAAG
    AATGCCAAAA TTACTATCCG AAGGATGAAG AAAATTCAGA TTCCAGTAGC TCGACAGGAA
    CCTGAGCCAG TGTCTGAAAA TGAGGACGAT AGCTATGATG AAGAGATACG GGATCCAAGG
    ACCAGCCGTG GTGCTTCCAG TGCCAACAGA AGGCATGAGA AGAGCTGGGT ACGAGACCGA
    AGTGCAAGCA GAGAAAGAAG CTTATCACCA AGATCTGACA GAAGATCGGT TACTTCCAGT
    CAGCCTGCAA AACCTACCAA AGTAACGTTG GTGAAATCAA GGAAAAATGA AGAGTATGGT
    CTACGGTTGG CAAGCCACAT ATTTGTGAAA GAAATATCAC AGGATAGCCT TGCAGCAAGA
    GATGGCAATA TTCAGGAAGG GGATGTTGTT CTGAAGATAA ATGGCACAGT GACAGAAAAT
    ATGTCCTTAG CAGATGCAAA AACGCTAATA GAAAGGTCCA AAGGCAAGTT GAAGATGGTG
    GTTCAGCGAG ATGAACGAGC TACACTGTTG AATGTCCCTG ATCTTTCTGA CAGTATTCAT
    TCTGCTAATG CTTCGGAAAG AGATGACATC TCAGAAATTC AGTCGCTGGC ATCAGATCAT
    TCCAACCGAT CACATGACAG GCCCCGCCGC AGTCGTTCAC GATCTCCTGA CCAGAGGTCA
    GAGCCTTCAG ACCATTCCAG ACATTCTCCA CAGCAGCCCA GCAACGGCAG TCTTCGAAGC
    AGAGAAGATG AAAGGGTAAC TAAACCAGGA GCCATCTCTA CACCTGTAAA GAATGCAGAT
    GATATTTCTA AAACTATGGA AGAGGTGTCA GTCGAAAGAA CTGAAAAACA AACACCACCG
    CTCCCAGAAC CAAAGCCAGT ATATGCACAA GGAGGTCAGC CAGATGTGGA TTTACCAGTC
    AGTCCGTCTG ATGGTCCTTT ACCCAATTCA ACCCATGAAG ATGGAATGCT TCGGCCAAGC
    ATGAAACTGG TAAAATTCAG AAAAGGAGAT AGTGTGGGCC TGCGGCTCGC TGGTGGCAAT
    GATGTTGGCA TATTTGTAGC TGGTGTTCTA GAGGACAGCC CTGCAGCGAA AGAAGGATTA
    GAGGAAGGAG ACCAAATTCT CAGGGTTAAT AACGTAGACT TCACAAATAT CATCAGAGAA
    GAAGCTGTCC TTTTTTTGCT TGATCTCCCT AAAGGCGAAG AAGTAACCAT TTTGGCACAG
    AAGAAGAAAG ATGTTTATCG TCGCATTGTT GAGTCTGATG TAGGGGATTC TTTCTATATC
    AGAACTCATT TTGAATATGA AAAGGAATCT CCTTATGGAC TAAGCTTCAA CAAAGGTGAA
    GTGTTTCGGG TTGTGGACAC GCTATATAAT GGCAAACTGG GTTCGTGGCT GGCAATTCGA
    ATTGGCAAGA ATCATAAGGA AGTTGAAAGA GGTATCATAC CTAACAAAAA CAGAGCTGAA
    CAACTAGCTA GTGTACAGTA CACGCTTCCA AAGACAGCAG GAGGAGATCG AGCGGATTTC
    TGGAGATTCC GAGGTTTGCG TAGCTCAAAA AGAAATCTCC GAAAAAGTAG AGAAGATCTT
    TCTGCTCAGC CTGTTCAGAC AAAGTTCCCT GCCTATGAAA GAGTTGTTCT TCGGGAAGCT
    GGGTTTCTCA GACCTGTGAC CATTTTTGGT CCAATAGCTG ATGTTGCACG AGAGAAACTA
    GCCAGAGAAG AACCAGATAT CTTTCAAATT GCAAAAAGTG AACCGAGAGA TGCTGGTACT
    GACCAACGTA GTTCTGGCAT TATTCGTCTT CATACGATAA AACAGATAAT TGATAGAGAC
    AAACACGCTT TATTAGATGT CACTCCTAAT GCAGTGGACC GTCTGAATTA TGCACAGTGG
    TATCCTATTG TAGTGTTTCT GAACCCTGAT TCTAAGCAGG GTGTGAAGAC CATGAGAATG
    AGGCTGTGCC CAGAATCACG AAAAAGTGCC AGGAAGCTAT ATGAAAGAGC TCACAAGCTA
    CGCAAAAATA ATCACCACCT CTTTACAACT ACCATTAACT TGAATTCAAT GAATGAAGGA
    TGGTATGGAG CTCTAAAAGA AGCAATTCAG CAACAACAAA ACCAACTGGT GTGGGTTTCT
    GAAGGAAAGG CAGATGGTGC TACAAGTGAT GACCTTGATT TACATGATGA CCGCCTGTCT
    TACCTCTCAG CTCCTGGTAG TGAATATTCT ATGTACAGTA CAGACAGCAG ACATACATCT
    GACTATGAAG ACACAGATAC AGAAGGTGGT GCTTATACTG ATCAAGAACT GGATGAAACT
    CTGAACGATG AGGTTGGAAC TCCTCCTGAA TCTGCTATTA CACGTTCTTC TGAACCCGTT
    AGGGAGGATT CTTCTGGAAT GCATCATGAA ACTCAAACTT ACACTAGTTA CGCATCTCAA
    GCTCAGCCTC AGCCAAATCT TAGAATAGAT TCTTCAGGAT TTAAAACAAC TGCTTCTCAG
    CCGAAAGCAG AAGCATCACC TGCAGTCCCT TACCTTTCCC CGTCGCCTGA ATCAAACCCA
    GCAACCTCAT CAACCTCTGC AGTAAATGCT AATGTAAACC TAACTAATGT CAGACTAGAG
    GAGCCTACTG CTGCTCCTTA CAACTCTTAT CAACCACAAG CGGGCCCCTT AAGAACATCA
    AGTACTGAGG GAGCTCATGT AGTGCTAAGA GATCAAGAGC CATCATCCTT ACCGCCGCAT
    ATAGATCCAG CAAAGGTATA CAGAAAGGAT CCATATATTA GTGAAGAAGC ATCCAGACAA
    AGCTATGTCT TAAAACAGCC AGCTGTTAAT CACCCAGTAC AAAGACAGGA AAGAGACCCA
    AATCTGATCT ATGAATCTCA GACTCAGTAT GCAGAAAAAC AACCAAGTAG GGAGTACGAG
    CAGTCAACAT ACAGGTATGA TTCTACAAAC TATGTAGATC AATTTTCTCG TGGGTATGAC
    CCTCGTCTAC ATTATGAAGA TCGTGCGCCT CCCTATGAGG AGCACTGGTC ATATTATGAT
    GAAAAACAGC CCTACAATCA ACCAAGAACA GCTTATGAAA GCCAGCCTCC TAGAGATCTT
    GATTCCAGAC AAAATACAGA AGAGAGCACA GAACGCAGCT ATTATCCGGC ACAACCCCGA
    TTTGAGGAAC CTCCTCCGAT GAGCTACGAT GGCAGACCTC GCTATGAGCA TGCACCCAAA
    AACTTCAGCT TGCCACAAGT GCGGTATGAA GATCAACATC CTGTTGGCTA TGACACACAT
    GCGCATGGTA GATATAAACA AGAAGCTCAG CCATACCAGT CAGCCATATC TCGATCTCCT
    GAACCCAAGC AGTACTTTGA TCCACATGTG AGGGGCTATG AGCAAGGTCC TCCTCAAGCT
    TACAATGCAA AAGCTGGACA GTTTGAGCCT TCTCATAGCA CTTCAGGTGT TTCTCTTCCT
    CCTCCCGCTT CTTCACAAAC AAAACCAGAA GTTTTGCCTT CCAACAGTAA ACCACTGCCT
    ACACCACCAT CTCTAGCAGA AGAAGAGGAG GATCCAGCCA TGAAACCACA GTCTGTGCGA
    AGTAGAGTTA AGATATTTGA ACGAAGAAGA TCGCCATCTC TGGAAAAAAT GAAGGACCCC
    AGTGACACGT CAGTTGTCAA ACCTCCAGAG CTAGCACCGA AGCCTACGCT CCCAGCCATG
    AGTGGTCCAA AACCAACTTC TCAAAGCCAG TATGAACATG ATAAAACAAC TTACAGGGCC
    CCAGAACCAC AGAGACCTCA AGTGAAGCCA CCTGAAGATA TCGTGCGTTC AAATCATTAT
    GATCCCGAAG AAGATGAAGA GTATTACAGA AAGCAGCTCT CATACTTTGA TCGCAGGAAT
    TTTGAAAATA AGCCTTCTGC ACAGGTTCCT GCCAGCCATC ATTCTGACTC CACTAAACCA
    ATACATTCAC AAAATCAGCT GAATTTCTCC AATTATTCTA AGGGGAAGCC AACTGATGCT
    GAACCAATGG ATAGATCTGT TGGTGACAAG CGCTATGAGC CAATCCCTCA AGTTACAACT
    CCTCCTCCTG CTCCTTCAGT ACAGTATACA CAACCTCAGT CAATTAATAG TCCTGTCCTG
    TCTCTCCCAG CGCATCACAA GCCTGCACTT TCTGAAGTTA ACTCTGTGTC TGACCCTCCT
    CCACCTCAGA ATAAGCCAGC AATTTTCAGA TCCTCTAGAG AGGACACTGT GCAGTCTACT
    TTTTATCCTC AGAAAAGCTT CCCTGACAAA GGTCCTGTTA ATGGAACTGA ACAGGTTCAG
    AAAACAGTCA CTCCTTCTTA CAACCGTTTT ACAACAAAAC CTTACACTAG TGCTGCGAGG
    CCCTTTGAGC GCAAGTTTGA AAGTCCTAAA TTCAACCATA ATCTCTTGCC AAATGAAGCT
    CAACATAAAC CAGAGTTGCC ATCAAAATCT CCAAATTCTC CTCAACCAAT TTTGAAAGCA
    CACAGCTCAT CACAGCCTCC TGAGTTTGAC AGTGGAATGG ACACCTTCAC TGTACAGGTT
    GACAAGCCTA AATATCAACC AAATAATGTA AATGCTGTGC CTAAAGCCAT TCCTGTAAGC
    CCTTCAGCAC TAGAGGATGA GGAGGAAGAA GACGGACACA CTGTTGTAGC CACAGCAAGA
    GGTGTATTCA ACAGCAATGG TGGCGTATTG AGCTCCATAG AGACTGGAGT TAGTATTATT
    ATACCACAAG GAGCCATTCC AGAGGGAATA GAGCAAGAAA TATATTTTAA AGTCTGCAGA
    GACAACAGTA TACTTCCACC TTTGGACAAA GAGAAAGGTG AAACACTTCT TAGCCCCTTG
    GTAATGTGTG GGCCTCACGG ACTAAAATTC CTGAAGCCAG TGGAGCTGCG CCTGCCACAC
    TGTGCGTCTA TGACCCCTGA TGGTTGGTCT TTTGCTCTAA AATCCTCTGA CTCCTCGTCG
    GGTGACCCCA AAACCTGGCA GAACAAGTCT CTTCCTGGAG ATCCAAACTA TCTTGTTGGA
    GCAAACTGTG TCTCAGTCCT AATTGACCAC TTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa40356
    Clone ID Related Accession (Same CDS sequence) XM_015278981.1
    Accession Version XM_015278981.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5205bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product tight junction protein ZO-1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763853.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAAGTATC AGAAATACCT GACTGTCTTG CAGATGGCCA TAGGGGTGAC CGCCTCCAAC 
    AGGGGCAGTC TGATGCCCTT AAAGAGGAAG CTGTGGGTAA CTCCATCTTC CGAAAATCCC
    AATGGTGCTA CCTGTAGTGT AAGTCAGGGA AAGCCTTCTT TAAGAAGAAT AAAAGGGAGA
    ATACACAGAA GCAAAAGCCT TGATAGCATT GATTTCTGTG AATTCACTAG TGCAACAATG
    GAAGAAACAG CTATATGGGA ACAACACACG GTGACTCTTC ACAGGGCTCC TGGATTTGGG
    TTTGGTATTG CAATATCTGG AGGAAGGGAT AATCCTCACT TTCAAAGTGG TGAGACCTCA
    ATAGTTATTT CTGATGTGCT GAAAGGAGGC CCAGCAGAAG GACTGCTACA AGAAAATGAC
    CGTGTTGCAA TGGTTAATGG AGTTTCAATG GATAATGTTG AGCACGCATT TGCTGTTCAG
    CAGCTGAGGA AAAGTGGGAA GAATGCCAAA ATTACTATCC GAAGGATGAA GAAAATTCAG
    ATTCCAGTAG CTCGACAGGA ACCTGAGCCA GTGTCTGAAA ATGAGGACGA TAGCTATGAT
    GAAGAGATAC GGGATCCAAG GACCAGCCGT GGTGCTTCCA GTGCCAACAG AAGGCATGAG
    AAGAGCTGGG TACGAGACCG AAGTGCAAGC AGAGAAAGAA GCTTATCACC AAGATCTGAC
    AGAAGATCGG TTACTTCCAG TCAGCCTGCA AAACCTACCA AAGTAACGTT GGTGAAATCA
    AGGAAAAATG AAGAGTATGG TCTACGGTTG GCAAGCCACA TATTTGTGAA AGAAATATCA
    CAGGATAGCC TTGCAGCAAG AGATGGCAAT ATTCAGGAAG GGGATGTTGT TCTGAAGATA
    AATGGCACAG TGACAGAAAA TATGTCCTTA GCAGATGCAA AAACGCTAAT AGAAAGGTCC
    AAAGGCAAGT TGAAGATGGT GGTTCAGCGA GATGAACGAG CTACACTGTT GAATGTCCCT
    GATCTTTCTG ACAGTATTCA TTCTGCTAAT GCTTCGGAAA GAGATGACAT CTCAGAAATT
    CAGTCGCTGG CATCAGATCA TTCCAACCGA TCACATGACA GGCCCCGCCG CAGTCGTTCA
    CGATCTCCTG ACCAGAGGTC AGAGCCTTCA GACCATTCCA GACATTCTCC ACAGCAGCCC
    AGCAACGGCA GTCTTCGAAG CAGAGAAGAT GAAAGGGTAA CTAAACCAGG AGCCATCTCT
    ACACCTGTAA AGAATGCAGA TGATATTTCT AAAACTATGG AAGAGGTGTC AGTCGAAAGA
    ACTGAAAAAC AAACACCACC GCTCCCAGAA CCAAAGCCAG TATATGCACA AGGAGGTCAG
    CCAGATGTGG ATTTACCAGT CAGTCCGTCT GATGGTCCTT TACCCAATTC AACCCATGAA
    GATGGAATGC TTCGGCCAAG CATGAAACTG GTAAAATTCA GAAAAGGAGA TAGTGTGGGC
    CTGCGGCTCG CTGGTGGCAA TGATGTTGGC ATATTTGTAG CTGGTGTTCT AGAGGACAGC
    CCTGCAGCGA AAGAAGGATT AGAGGAAGGA GACCAAATTC TCAGGGTTAA TAACGTAGAC
    TTCACAAATA TCATCAGAGA AGAAGCTGTC CTTTTTTTGC TTGATCTCCC TAAAGGCGAA
    GAAGTAACCA TTTTGGCACA GAAGAAGAAA GATGTTTATC GTCGCATTGT TGAGTCTGAT
    GTAGGGGATT CTTTCTATAT CAGAACTCAT TTTGAATATG AAAAGGAATC TCCTTATGGA
    CTAAGCTTCA ACAAAGGTGA AGTGTTTCGG GTTGTGGACA CGCTATATAA TGGCAAACTG
    GGTTCGTGGC TGGCAATTCG AATTGGCAAG AATCATAAGG AAGTTGAAAG AGGTATCATA
    CCTAACAAAA ACAGAGCTGA ACAACTAGCT AGTGTACAGT ACACGCTTCC AAAGACAGCA
    GGAGGAGATC GAGCGGATTT CTGGAGATTC CGAGGTTTGC GTAGCTCAAA AAGAAATCTC
    CGAAAAAGTA GAGAAGATCT TTCTGCTCAG CCTGTTCAGA CAAAGTTCCC TGCCTATGAA
    AGAGTTGTTC TTCGGGAAGC TGGGTTTCTC AGACCTGTGA CCATTTTTGG TCCAATAGCT
    GATGTTGCAC GAGAGAAACT AGCCAGAGAA GAACCAGATA TCTTTCAAAT TGCAAAAAGT
    GAACCGAGAG ATGCTGGTAC TGACCAACGT AGTTCTGGCA TTATTCGTCT TCATACGATA
    AAACAGATAA TTGATAGAGA CAAACACGCT TTATTAGATG TCACTCCTAA TGCAGTGGAC
    CGTCTGAATT ATGCACAGTG GTATCCTATT GTAGTGTTTC TGAACCCTGA TTCTAAGCAG
    GGTGTGAAGA CCATGAGAAT GAGGCTGTGC CCAGAATCAC GAAAAAGTGC CAGGAAGCTA
    TATGAAAGAG CTCACAAGCT ACGCAAAAAT AATCACCACC TCTTTACAAC TACCATTAAC
    TTGAATTCAA TGAATGAAGG ATGGTATGGA GCTCTAAAAG AAGCAATTCA GCAACAACAA
    AACCAACTGG TGTGGGTTTC TGAAGGAAAG GCAGATGGTG CTACAAGTGA TGACCTTGAT
    TTACATGATG ACCGCCTGTC TTACCTCTCA GCTCCTGGTA GTGAATATTC TATGTACAGT
    ACAGACAGCA GACATACATC TGACTATGAA GACACAGATA CAGAAGGTGG TGCTTATACT
    GATCAAGAAC TGGATGAAAC TCTGAACGAT GAGGTTGGAA CTCCTCCTGA ATCTGCTATT
    ACACGTTCTT CTGAACCCGT TAGGGAGGAT TCTTCTGGAA TGCATCATGA AACTCAAACT
    TACACTAGTT ACGCATCTCA AGCTCAGCCT CAGCCAAATC TTAGAATAGA TTCTTCAGGA
    TTTAAAACAA CTGCTTCTCA GCCGGTATAC AGAAAGGATC CATATATTAG TGAAGAAGCA
    TCCAGACAAA GCTATGTCTT AAAACAGCCA GCTGTTAATC ACCCAGTACA AAGACAGGAA
    AGAGACCCAA ATCTGATCTA TGAATCTCAG ACTCAGTATG CAGAAAAACA ACCAAGTAGG
    GAGTACGAGC AGTCAACATA CAGGTATGAT TCTACAAACT ATGTAGATCA ATTTTCTCGT
    GGGTATGACC CTCGTCTACA TTATGAAGAT CGTGCGCCTC CCTATGAGGA GCACTGGTCA
    TATTATGATG AAAAACAGCC CTACAATCAA CCAAGAACAG CTTATGAAAG CCAGCCTCCT
    AGAGATCTTG ATTCCAGACA AAATACAGAA GAGAGCACAG AACGCAGCTA TTATCCGGCA
    CAACCCCGAT TTGAGGAACC TCCTCCGATG AGCTACGATG GCAGACCTCG CTATGAGCAT
    GCACCCAAAA ACTTCAGCTT GCCACAAGTG CGGTATGAAG ATCAACATCC TGTTGGCTAT
    GACACACATG CGCATGGTAG ATATAAACAA GAAGCTCAGC CATACCAGTC AGCCATATCT
    CGATCTCCTG AACCCAAGCA GTACTTTGAT CCACATGTGA GGGGCTATGA GCAAGGTCCT
    CCTCAAGCTT ACAATGCAAA AGCTGGACAG TTTGAGCCTT CTCATAGCAC TTCAGGTGTT
    TCTCTTCCTC CTCCCGCTTC TTCACAAACA AAACCAGAAG TTTTGCCTTC CAACAGTAAA
    CCACTGCCTA CACCACCATC TCTAGCAGAA GAAGAGGAGG ATCCAGCCAT GAAACCACAG
    TCTGTGCGAA GTAGAGTTAA GATATTTGAA CGAAGAAGAT CGCCATCTCT GGAAAAAATG
    AAGGACCCCA GTGACACGTC AGTTGTCAAA CCTCCAGAGC TAGCACCGAA GCCTACGCTC
    CCAGCCATGA GTGGTCCAAA ACCAACTTCT CAAAGCCAGT ATGAACATGA TAAAACAACT
    TACAGGGCCC CAGAACCACA GAGACCTCAA GTGAAGCCAC CTGAAGATAT CGTGCGTTCA
    AATCATTATG ATCCCGAAGA AGATGAAGAG TATTACAGAA AGCAGCTCTC ATACTTTGAT
    CGCAGGAATT TTGAAAATAA GCCTTCTGCA CAGGTTCCTG CCAGCCATCA TTCTGACTCC
    ACTAAACCAA TACATTCACA AAATCAGCTG AATTTCTCCA ATTATTCTAA GGGGAAGCCA
    ACTGATGCTG AACCAATGGA TAGATCTGTT GGTGACAAGC GCTATGAGCC AATCCCTCAA
    GTTACAACTC CTCCTCCTGC TCCTTCAGTA CAGTATACAC AACCTCAGTC AATTAATAGT
    CCTGTCCTGT CTCTCCCAGC GCATCACAAG CCTGCACTTT CTGAAGTTAA CTCTGTGTCT
    GACCCTCCTC CACCTCAGAA TAAGCCAGCA ATTTTCAGAT CCTCTAGAGA GGACACTGTG
    CAGTCTACTT TTTATCCTCA GAAAAGCTTC CCTGACAAAG GTCCTGTTAA TGGAACTGAA
    CAGGTTCAGA AAACAGTCAC TCCTTCTTAC AACCGTTTTA CAACAAAACC TTACACTAGT
    GCTGCGAGGC CCTTTGAGCG CAAGTTTGAA AGTCCTAAAT TCAACCATAA TCTCTTGCCA
    AATGAAGCTC AACATAAACC AGAGTTGCCA TCAAAATCTC CAAATTCTCC TCAACCAATT
    TTGAAAGCAC ACAGCTCATC ACAGCCTCCT GAGTTTGACA GTGGAATGGA CACCTTCACT
    GTACAGGTTG ACAAGCCTAA ATATCAACCA AATAATGTAA ATGCTGTGCC TAAAGCCATT
    CCTGTAAGCC CTTCAGCACT AGAGGATGAG GAGGAAGAAG ACGGACACAC TGTTGTAGCC
    ACAGCAAGAG GTGTATTCAA CAGCAATGGT GGCGTATTGA GCTCCATAGA GACTGGAGTT
    AGTATTATTA TACCACAAGG AGCCATTCCA GAGGGAATAG AGCAAGAAAT ATATTTTAAA
    GTCTGCAGAG ACAACAGTAT ACTTCCACCT TTGGACAAAG AGAAAGGTGA AACACTTCTT
    AGCCCCTTGG TAATGTGTGG GCCTCACGGA CTAAAATTCC TGAAGCCAGT GGAGCTGCGC
    CTGCCACACT GTGACCCCAA AACCTGGCAG AACAAGTCTC TTCCTGGAGA TCCAAACTAT
    CTTGTTGGAG CAAACTGTGT CTCAGTCCTA ATTGACCACT TCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015134467.1
    CDS16..5220
    Translation

    Target ORF information:

    RefSeq Version XM_015278981.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus tight junction protein 1 (TJP1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015278981.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAAGTATC AGAAATACCT GACTGTCTTG CAGATGGCCA TAGGGGTGAC CGCCTCCAAC 
    AGGGGCAGTC TGATGCCCTT AAAGAGGAAG CTGTGGGTAA CTCCATCTTC CGAAAATCCC
    AATGGTGCTA CCTGTAGTGT AAGTCAGGGA AAGCCTTCTT TAAGAAGAAT AAAAGGGAGA
    ATACACAGAA GCAAAAGCCT TGATAGCATT GATTTCTGTG AATTCACTAG TGCAACAATG
    GAAGAAACAG CTATATGGGA ACAACACACG GTGACTCTTC ACAGGGCTCC TGGATTTGGG
    TTTGGTATTG CAATATCTGG AGGAAGGGAT AATCCTCACT TTCAAAGTGG TGAGACCTCA
    ATAGTTATTT CTGATGTGCT GAAAGGAGGC CCAGCAGAAG GACTGCTACA AGAAAATGAC
    CGTGTTGCAA TGGTTAATGG AGTTTCAATG GATAATGTTG AGCACGCATT TGCTGTTCAG
    CAGCTGAGGA AAAGTGGGAA GAATGCCAAA ATTACTATCC GAAGGATGAA GAAAATTCAG
    ATTCCAGTAG CTCGACAGGA ACCTGAGCCA GTGTCTGAAA ATGAGGACGA TAGCTATGAT
    GAAGAGATAC GGGATCCAAG GACCAGCCGT GGTGCTTCCA GTGCCAACAG AAGGCATGAG
    AAGAGCTGGG TACGAGACCG AAGTGCAAGC AGAGAAAGAA GCTTATCACC AAGATCTGAC
    AGAAGATCGG TTACTTCCAG TCAGCCTGCA AAACCTACCA AAGTAACGTT GGTGAAATCA
    AGGAAAAATG AAGAGTATGG TCTACGGTTG GCAAGCCACA TATTTGTGAA AGAAATATCA
    CAGGATAGCC TTGCAGCAAG AGATGGCAAT ATTCAGGAAG GGGATGTTGT TCTGAAGATA
    AATGGCACAG TGACAGAAAA TATGTCCTTA GCAGATGCAA AAACGCTAAT AGAAAGGTCC
    AAAGGCAAGT TGAAGATGGT GGTTCAGCGA GATGAACGAG CTACACTGTT GAATGTCCCT
    GATCTTTCTG ACAGTATTCA TTCTGCTAAT GCTTCGGAAA GAGATGACAT CTCAGAAATT
    CAGTCGCTGG CATCAGATCA TTCCAACCGA TCACATGACA GGCCCCGCCG CAGTCGTTCA
    CGATCTCCTG ACCAGAGGTC AGAGCCTTCA GACCATTCCA GACATTCTCC ACAGCAGCCC
    AGCAACGGCA GTCTTCGAAG CAGAGAAGAT GAAAGGGTAA CTAAACCAGG AGCCATCTCT
    ACACCTGTAA AGAATGCAGA TGATATTTCT AAAACTATGG AAGAGGTGTC AGTCGAAAGA
    ACTGAAAAAC AAACACCACC GCTCCCAGAA CCAAAGCCAG TATATGCACA AGGAGGTCAG
    CCAGATGTGG ATTTACCAGT CAGTCCGTCT GATGGTCCTT TACCCAATTC AACCCATGAA
    GATGGAATGC TTCGGCCAAG CATGAAACTG GTAAAATTCA GAAAAGGAGA TAGTGTGGGC
    CTGCGGCTCG CTGGTGGCAA TGATGTTGGC ATATTTGTAG CTGGTGTTCT AGAGGACAGC
    CCTGCAGCGA AAGAAGGATT AGAGGAAGGA GACCAAATTC TCAGGGTTAA TAACGTAGAC
    TTCACAAATA TCATCAGAGA AGAAGCTGTC CTTTTTTTGC TTGATCTCCC TAAAGGCGAA
    GAAGTAACCA TTTTGGCACA GAAGAAGAAA GATGTTTATC GTCGCATTGT TGAGTCTGAT
    GTAGGGGATT CTTTCTATAT CAGAACTCAT TTTGAATATG AAAAGGAATC TCCTTATGGA
    CTAAGCTTCA ACAAAGGTGA AGTGTTTCGG GTTGTGGACA CGCTATATAA TGGCAAACTG
    GGTTCGTGGC TGGCAATTCG AATTGGCAAG AATCATAAGG AAGTTGAAAG AGGTATCATA
    CCTAACAAAA ACAGAGCTGA ACAACTAGCT AGTGTACAGT ACACGCTTCC AAAGACAGCA
    GGAGGAGATC GAGCGGATTT CTGGAGATTC CGAGGTTTGC GTAGCTCAAA AAGAAATCTC
    CGAAAAAGTA GAGAAGATCT TTCTGCTCAG CCTGTTCAGA CAAAGTTCCC TGCCTATGAA
    AGAGTTGTTC TTCGGGAAGC TGGGTTTCTC AGACCTGTGA CCATTTTTGG TCCAATAGCT
    GATGTTGCAC GAGAGAAACT AGCCAGAGAA GAACCAGATA TCTTTCAAAT TGCAAAAAGT
    GAACCGAGAG ATGCTGGTAC TGACCAACGT AGTTCTGGCA TTATTCGTCT TCATACGATA
    AAACAGATAA TTGATAGAGA CAAACACGCT TTATTAGATG TCACTCCTAA TGCAGTGGAC
    CGTCTGAATT ATGCACAGTG GTATCCTATT GTAGTGTTTC TGAACCCTGA TTCTAAGCAG
    GGTGTGAAGA CCATGAGAAT GAGGCTGTGC CCAGAATCAC GAAAAAGTGC CAGGAAGCTA
    TATGAAAGAG CTCACAAGCT ACGCAAAAAT AATCACCACC TCTTTACAAC TACCATTAAC
    TTGAATTCAA TGAATGAAGG ATGGTATGGA GCTCTAAAAG AAGCAATTCA GCAACAACAA
    AACCAACTGG TGTGGGTTTC TGAAGGAAAG GCAGATGGTG CTACAAGTGA TGACCTTGAT
    TTACATGATG ACCGCCTGTC TTACCTCTCA GCTCCTGGTA GTGAATATTC TATGTACAGT
    ACAGACAGCA GACATACATC TGACTATGAA GACACAGATA CAGAAGGTGG TGCTTATACT
    GATCAAGAAC TGGATGAAAC TCTGAACGAT GAGGTTGGAA CTCCTCCTGA ATCTGCTATT
    ACACGTTCTT CTGAACCCGT TAGGGAGGAT TCTTCTGGAA TGCATCATGA AACTCAAACT
    TACACTAGTT ACGCATCTCA AGCTCAGCCT CAGCCAAATC TTAGAATAGA TTCTTCAGGA
    TTTAAAACAA CTGCTTCTCA GCCGGTATAC AGAAAGGATC CATATATTAG TGAAGAAGCA
    TCCAGACAAA GCTATGTCTT AAAACAGCCA GCTGTTAATC ACCCAGTACA AAGACAGGAA
    AGAGACCCAA ATCTGATCTA TGAATCTCAG ACTCAGTATG CAGAAAAACA ACCAAGTAGG
    GAGTACGAGC AGTCAACATA CAGGTATGAT TCTACAAACT ATGTAGATCA ATTTTCTCGT
    GGGTATGACC CTCGTCTACA TTATGAAGAT CGTGCGCCTC CCTATGAGGA GCACTGGTCA
    TATTATGATG AAAAACAGCC CTACAATCAA CCAAGAACAG CTTATGAAAG CCAGCCTCCT
    AGAGATCTTG ATTCCAGACA AAATACAGAA GAGAGCACAG AACGCAGCTA TTATCCGGCA
    CAACCCCGAT TTGAGGAACC TCCTCCGATG AGCTACGATG GCAGACCTCG CTATGAGCAT
    GCACCCAAAA ACTTCAGCTT GCCACAAGTG CGGTATGAAG ATCAACATCC TGTTGGCTAT
    GACACACATG CGCATGGTAG ATATAAACAA GAAGCTCAGC CATACCAGTC AGCCATATCT
    CGATCTCCTG AACCCAAGCA GTACTTTGAT CCACATGTGA GGGGCTATGA GCAAGGTCCT
    CCTCAAGCTT ACAATGCAAA AGCTGGACAG TTTGAGCCTT CTCATAGCAC TTCAGGTGTT
    TCTCTTCCTC CTCCCGCTTC TTCACAAACA AAACCAGAAG TTTTGCCTTC CAACAGTAAA
    CCACTGCCTA CACCACCATC TCTAGCAGAA GAAGAGGAGG ATCCAGCCAT GAAACCACAG
    TCTGTGCGAA GTAGAGTTAA GATATTTGAA CGAAGAAGAT CGCCATCTCT GGAAAAAATG
    AAGGACCCCA GTGACACGTC AGTTGTCAAA CCTCCAGAGC TAGCACCGAA GCCTACGCTC
    CCAGCCATGA GTGGTCCAAA ACCAACTTCT CAAAGCCAGT ATGAACATGA TAAAACAACT
    TACAGGGCCC CAGAACCACA GAGACCTCAA GTGAAGCCAC CTGAAGATAT CGTGCGTTCA
    AATCATTATG ATCCCGAAGA AGATGAAGAG TATTACAGAA AGCAGCTCTC ATACTTTGAT
    CGCAGGAATT TTGAAAATAA GCCTTCTGCA CAGGTTCCTG CCAGCCATCA TTCTGACTCC
    ACTAAACCAA TACATTCACA AAATCAGCTG AATTTCTCCA ATTATTCTAA GGGGAAGCCA
    ACTGATGCTG AACCAATGGA TAGATCTGTT GGTGACAAGC GCTATGAGCC AATCCCTCAA
    GTTACAACTC CTCCTCCTGC TCCTTCAGTA CAGTATACAC AACCTCAGTC AATTAATAGT
    CCTGTCCTGT CTCTCCCAGC GCATCACAAG CCTGCACTTT CTGAAGTTAA CTCTGTGTCT
    GACCCTCCTC CACCTCAGAA TAAGCCAGCA ATTTTCAGAT CCTCTAGAGA GGACACTGTG
    CAGTCTACTT TTTATCCTCA GAAAAGCTTC CCTGACAAAG GTCCTGTTAA TGGAACTGAA
    CAGGTTCAGA AAACAGTCAC TCCTTCTTAC AACCGTTTTA CAACAAAACC TTACACTAGT
    GCTGCGAGGC CCTTTGAGCG CAAGTTTGAA AGTCCTAAAT TCAACCATAA TCTCTTGCCA
    AATGAAGCTC AACATAAACC AGAGTTGCCA TCAAAATCTC CAAATTCTCC TCAACCAATT
    TTGAAAGCAC ACAGCTCATC ACAGCCTCCT GAGTTTGACA GTGGAATGGA CACCTTCACT
    GTACAGGTTG ACAAGCCTAA ATATCAACCA AATAATGTAA ATGCTGTGCC TAAAGCCATT
    CCTGTAAGCC CTTCAGCACT AGAGGATGAG GAGGAAGAAG ACGGACACAC TGTTGTAGCC
    ACAGCAAGAG GTGTATTCAA CAGCAATGGT GGCGTATTGA GCTCCATAGA GACTGGAGTT
    AGTATTATTA TACCACAAGG AGCCATTCCA GAGGGAATAG AGCAAGAAAT ATATTTTAAA
    GTCTGCAGAG ACAACAGTAT ACTTCCACCT TTGGACAAAG AGAAAGGTGA AACACTTCTT
    AGCCCCTTGG TAATGTGTGG GCCTCACGGA CTAAAATTCC TGAAGCCAGT GGAGCTGCGC
    CTGCCACACT GTGACCCCAA AACCTGGCAG AACAAGTCTC TTCCTGGAGA TCCAAACTAT
    CTTGTTGGAG CAAACTGTGT CTCAGTCCTA ATTGACCACT TCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa40353
    Clone ID Related Accession (Same CDS sequence) XM_015278978.1
    Accession Version XM_015278978.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5439bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product tight junction protein ZO-1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763853.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGCCGCCCC CGCACGTGCC GCCGCTCGGG GAAAGCCGGG CCCGGCGGCG GGACGGGGGT 
    CCGGGCGGGG CGGGGCTCTC CCCCGCGCGG CGGGATGGGC CGCGCCGCAG TCAGGCGCGT
    CCGGGGCTGC GCGGCACGCG GCGCCGTGAG AGTGCAACAA TGGAAGAAAC AGCTATATGG
    GAACAACACA CGGTGACTCT TCACAGGGCT CCTGGATTTG GGTTTGGTAT TGCAATATCT
    GGAGGAAGGG ATAATCCTCA CTTTCAAAGT GGTGAGACCT CAATAGTTAT TTCTGATGTG
    CTGAAAGGAG GCCCAGCAGA AGGACTGCTA CAAGAAAATG ACCGTGTTGC AATGGTTAAT
    GGAGTTTCAA TGGATAATGT TGAGCACGCA TTTGCTGTTC AGCAGCTGAG GAAAAGTGGG
    AAGAATGCCA AAATTACTAT CCGAAGGATG AAGAAAATTC AGATTCCAGT AGCTCGACAG
    GAACCTGAGC CAGTGTCTGA AAATGAGGAC GATAGCTATG ATGAAGAGAT ACGGGATCCA
    AGGACCAGCC GTGGTGCTTC CAGTGCCAAC AGAAGGCATG AGAAGAGCTG GGTACGAGAC
    CGAAGTGCAA GCAGAGAAAG AAGCTTATCA CCAAGATCTG ACAGAAGATC GGTTACTTCC
    AGTCAGCCTG CAAAACCTAC CAAAGTAACG TTGGTGAAAT CAAGGAAAAA TGAAGAGTAT
    GGTCTACGGT TGGCAAGCCA CATATTTGTG AAAGAAATAT CACAGGATAG CCTTGCAGCA
    AGAGATGGCA ATATTCAGGA AGGGGATGTT GTTCTGAAGA TAAATGGCAC AGTGACAGAA
    AATATGTCCT TAGCAGATGC AAAAACGCTA ATAGAAAGGT CCAAAGGCAA GTTGAAGATG
    GTGGTTCAGC GAGATGAACG AGCTACACTG TTGAATGTCC CTGATCTTTC TGACAGTATT
    CATTCTGCTA ATGCTTCGGA AAGAGATGAC ATCTCAGAAA TTCAGTCGCT GGCATCAGAT
    CATTCCAACC GATCACATGA CAGGCCCCGC CGCAGTCGTT CACGATCTCC TGACCAGAGG
    TCAGAGCCTT CAGACCATTC CAGACATTCT CCACAGCAGC CCAGCAACGG CAGTCTTCGA
    AGCAGAGAAG ATGAAAGGGT AACTAAACCA GGAGCCATCT CTACACCTGT AAAGAATGCA
    GATGATATTT CTAAAACTAT GGAAGAGGTG TCAGTCGAAA GAACTGAAAA ACAAACACCA
    CCGCTCCCAG AACCAAAGCC AGTATATGCA CAAGGAGGTC AGCCAGATGT GGATTTACCA
    GTCAGTCCGT CTGATGGTCC TTTACCCAAT TCAACCCATG AAGATGGAAT GCTTCGGCCA
    AGCATGAAAC TGGTAAAATT CAGAAAAGGA GATAGTGTGG GCCTGCGGCT CGCTGGTGGC
    AATGATGTTG GCATATTTGT AGCTGGTGTT CTAGAGGACA GCCCTGCAGC GAAAGAAGGA
    TTAGAGGAAG GAGACCAAAT TCTCAGGGTT AATAACGTAG ACTTCACAAA TATCATCAGA
    GAAGAAGCTG TCCTTTTTTT GCTTGATCTC CCTAAAGGCG AAGAAGTAAC CATTTTGGCA
    CAGAAGAAGA AAGATGTTTA TCGTCGCATT GTTGAGTCTG ATGTAGGGGA TTCTTTCTAT
    ATCAGAACTC ATTTTGAATA TGAAAAGGAA TCTCCTTATG GACTAAGCTT CAACAAAGGT
    GAAGTGTTTC GGGTTGTGGA CACGCTATAT AATGGCAAAC TGGGTTCGTG GCTGGCAATT
    CGAATTGGCA AGAATCATAA GGAAGTTGAA AGAGGTATCA TACCTAACAA AAACAGAGCT
    GAACAACTAG CTAGTGTACA GTACACGCTT CCAAAGACAG CAGGAGGAGA TCGAGCGGAT
    TTCTGGAGAT TCCGAGGTTT GCGTAGCTCA AAAAGAAATC TCCGAAAAAG TAGAGAAGAT
    CTTTCTGCTC AGCCTGTTCA GACAAAGTTC CCTGCCTATG AAAGAGTTGT TCTTCGGGAA
    GCTGGGTTTC TCAGACCTGT GACCATTTTT GGTCCAATAG CTGATGTTGC ACGAGAGAAA
    CTAGCCAGAG AAGAACCAGA TATCTTTCAA ATTGCAAAAA GTGAACCGAG AGATGCTGGT
    ACTGACCAAC GTAGTTCTGG CATTATTCGT CTTCATACGA TAAAACAGAT AATTGATAGA
    GACAAACACG CTTTATTAGA TGTCACTCCT AATGCAGTGG ACCGTCTGAA TTATGCACAG
    TGGTATCCTA TTGTAGTGTT TCTGAACCCT GATTCTAAGC AGGGTGTGAA GACCATGAGA
    ATGAGGCTGT GCCCAGAATC ACGAAAAAGT GCCAGGAAGC TATATGAAAG AGCTCACAAG
    CTACGCAAAA ATAATCACCA CCTCTTTACA ACTACCATTA ACTTGAATTC AATGAATGAA
    GGATGGTATG GAGCTCTAAA AGAAGCAATT CAGCAACAAC AAAACCAACT GGTGTGGGTT
    TCTGAAGGAA AGGCAGATGG TGCTACAAGT GATGACCTTG ATTTACATGA TGACCGCCTG
    TCTTACCTCT CAGCTCCTGG TAGTGAATAT TCTATGTACA GTACAGACAG CAGACATACA
    TCTGACTATG AAGACACAGA TACAGAAGGT GGTGCTTATA CTGATCAAGA ACTGGATGAA
    ACTCTGAACG ATGAGGTTGG AACTCCTCCT GAATCTGCTA TTACACGTTC TTCTGAACCC
    GTTAGGGAGG ATTCTTCTGG AATGCATCAT GAAACTCAAA CTTACACTAG TTACGCATCT
    CAAGCTCAGC CTCAGCCAAA TCTTAGAATA GATTCTTCAG GATTTAAAAC AACTGCTTCT
    CAGCCGAAAG CAGAAGCATC ACCTGCAGTC CCTTACCTTT CCCCGTCGCC TGAATCAAAC
    CCAGCAACCT CATCAACCTC TGCAGTAAAT GCTAATGTAA ACCTAACTAA TGTCAGACTA
    GAGGAGCCTA CTGCTGCTCC TTACAACTCT TATCAACCAC AAGCGGGCCC CTTAAGAACA
    TCAAGTACTG AGGGAGCTCA TGTAGTGCTA AGAGATCAAG AGCCATCATC CTTACCGCCG
    CATATAGATC CAGCAAAGGT ATACAGAAAG GATCCATATA TTAGTGAAGA AGCATCCAGA
    CAAAGCTATG TCTTAAAACA GCCAGCTGTT AATCACCCAG TACAAAGACA GGAAAGAGAC
    CCAAATCTGA TCTATGAATC TCAGACTCAG TATGCAGAAA AACAACCAAG TAGGGAGTAC
    GAGCAGTCAA CATACAGGTA TGATTCTACA AACTATGTAG ATCAATTTTC TCGTGGGTAT
    GACCCTCGTC TACATTATGA AGATCGTGCG CCTCCCTATG AGGAGCACTG GTCATATTAT
    GATGAAAAAC AGCCCTACAA TCAACCAAGA ACAGCTTATG AAAGCCAGCC TCCTAGAGAT
    CTTGATTCCA GACAAAATAC AGAAGAGAGC ACAGAACGCA GCTATTATCC GGCACAACCC
    CGATTTGAGG AACCTCCTCC GATGAGCTAC GATGGCAGAC CTCGCTATGA GCATGCACCC
    AAAAACTTCA GCTTGCCACA AGTGCGGTAT GAAGATCAAC ATCCTGTTGG CTATGACACA
    CATGCGCATG GTAGATATAA ACAAGAAGCT CAGCCATACC AGTCAGCCAT ATCTCGATCT
    CCTGAACCCA AGCAGTACTT TGATCCACAT GTGAGGGGCT ATGAGCAAGG TCCTCCTCAA
    GCTTACAATG CAAAAGCTGG ACAGTTTGAG CCTTCTCATA GCACTTCAGG TGTTTCTCTT
    CCTCCTCCCG CTTCTTCACA AACAAAACCA GAAGTTTTGC CTTCCAACAG TAAACCACTG
    CCTACACCAC CATCTCTAGC AGAAGAAGAG GAGGATCCAG CCATGAAACC ACAGTCTGTG
    CGAAGTAGAG TTAAGATATT TGAACGAAGA AGATCGCCAT CTCTGGAAAA AATGAAGGAC
    CCCAGTGACA CGTCAGTTGT CAAACCTCCA GAGCTAGCAC CGAAGCCTAC GCTCCCAGCC
    ATGAGTGGTC CAAAACCAAC TTCTCAAAGC CAGTATGAAC ATGATAAAAC AACTTACAGG
    GCCCCAGAAC CACAGAGACC TCAAGTGAAG CCACCTGAAG ATATCGTGCG TTCAAATCAT
    TATGATCCCG AAGAAGATGA AGAGTATTAC AGAAAGCAGC TCTCATACTT TGATCGCAGG
    AATTTTGAAA ATAAGCCTTC TGCACAGGTT CCTGCCAGCC ATCATTCTGA CTCCACTAAA
    CCAATACATT CACAAAATCA GCTGAATTTC TCCAATTATT CTAAGGGGAA GCCAACTGAT
    GCTGAACCAA TGGATAGATC TGTTGGTGAC AAGCGCTATG AGCCAATCCC TCAAGTTACA
    ACTCCTCCTC CTGCTCCTTC AGTACAGTAT ACACAACCTC AGTCAATTAA TAGTCCTGTC
    CTGTCTCTCC CAGCGCATCA CAAGCCTGCA CTTTCTGAAG TTAACTCTGT GTCTGACCCT
    CCTCCACCTC AGAATAAGCC AGCAATTTTC AGATCCTCTA GAGAGGACAC TGTGCAGTCT
    ACTTTTTATC CTCAGAAAAG CTTCCCTGAC AAAGGTCCTG TTAATGGAAC TGAACAGGTT
    CAGAAAACAG TCACTCCTTC TTACAACCGT TTTACAACAA AACCTTACAC TAGTGCTGCG
    AGGCCCTTTG AGCGCAAGTT TGAAAGTCCT AAATTCAACC ATAATCTCTT GCCAAATGAA
    GCTCAACATA AACCAGAGTT GCCATCAAAA TCTCCAAATT CTCCTCAACC AATTTTGAAA
    GCACACAGCT CATCACAGCC TCCTGAGTTT GACAGTGGAA TGGACACCTT CACTGTACAG
    GTTGACAAGC CTAAATATCA ACCAAATAAT GTAAATGCTG TGCCTAAAGC CATTCCTGTA
    AGCCCTTCAG CACTAGAGGA TGAGGAGGAA GAAGACGGAC ACACTGTTGT AGCCACAGCA
    AGAGGTGTAT TCAACAGCAA TGGTGGCGTA TTGAGCTCCA TAGAGACTGG AGTTAGTATT
    ATTATACCAC AAGGAGCCAT TCCAGAGGGA ATAGAGCAAG AAATATATTT TAAAGTCTGC
    AGAGACAACA GTATACTTCC ACCTTTGGAC AAAGAGAAAG GTGAAACACT TCTTAGCCCC
    TTGGTAATGT GTGGGCCTCA CGGACTAAAA TTCCTGAAGC CAGTGGAGCT GCGCCTGCCA
    CACTGTGCGT CTATGACCCC TGATGGTTGG TCTTTTGCTC TAAAATCCTC TGACTCCTCG
    TCGGGTGACC CCAAAACCTG GCAGAACAAG TCTCTTCCTG GAGATCCAAA CTATCTTGTT
    GGAGCAAACT GTGTCTCAGT CCTAATTGAC CACTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015134464.1
    CDS16..5454
    Translation

    Target ORF information:

    RefSeq Version XM_015278978.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus tight junction protein 1 (TJP1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015278978.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGCCGCCCC CGCACGTGCC GCCGCTCGGG GAAAGCCGGG CCCGGCGGCG GGACGGGGGT 
    CCGGGCGGGG CGGGGCTCTC CCCCGCGCGG CGGGATGGGC CGCGCCGCAG TCAGGCGCGT
    CCGGGGCTGC GCGGCACGCG GCGCCGTGAG AGTGCAACAA TGGAAGAAAC AGCTATATGG
    GAACAACACA CGGTGACTCT TCACAGGGCT CCTGGATTTG GGTTTGGTAT TGCAATATCT
    GGAGGAAGGG ATAATCCTCA CTTTCAAAGT GGTGAGACCT CAATAGTTAT TTCTGATGTG
    CTGAAAGGAG GCCCAGCAGA AGGACTGCTA CAAGAAAATG ACCGTGTTGC AATGGTTAAT
    GGAGTTTCAA TGGATAATGT TGAGCACGCA TTTGCTGTTC AGCAGCTGAG GAAAAGTGGG
    AAGAATGCCA AAATTACTAT CCGAAGGATG AAGAAAATTC AGATTCCAGT AGCTCGACAG
    GAACCTGAGC CAGTGTCTGA AAATGAGGAC GATAGCTATG ATGAAGAGAT ACGGGATCCA
    AGGACCAGCC GTGGTGCTTC CAGTGCCAAC AGAAGGCATG AGAAGAGCTG GGTACGAGAC
    CGAAGTGCAA GCAGAGAAAG AAGCTTATCA CCAAGATCTG ACAGAAGATC GGTTACTTCC
    AGTCAGCCTG CAAAACCTAC CAAAGTAACG TTGGTGAAAT CAAGGAAAAA TGAAGAGTAT
    GGTCTACGGT TGGCAAGCCA CATATTTGTG AAAGAAATAT CACAGGATAG CCTTGCAGCA
    AGAGATGGCA ATATTCAGGA AGGGGATGTT GTTCTGAAGA TAAATGGCAC AGTGACAGAA
    AATATGTCCT TAGCAGATGC AAAAACGCTA ATAGAAAGGT CCAAAGGCAA GTTGAAGATG
    GTGGTTCAGC GAGATGAACG AGCTACACTG TTGAATGTCC CTGATCTTTC TGACAGTATT
    CATTCTGCTA ATGCTTCGGA AAGAGATGAC ATCTCAGAAA TTCAGTCGCT GGCATCAGAT
    CATTCCAACC GATCACATGA CAGGCCCCGC CGCAGTCGTT CACGATCTCC TGACCAGAGG
    TCAGAGCCTT CAGACCATTC CAGACATTCT CCACAGCAGC CCAGCAACGG CAGTCTTCGA
    AGCAGAGAAG ATGAAAGGGT AACTAAACCA GGAGCCATCT CTACACCTGT AAAGAATGCA
    GATGATATTT CTAAAACTAT GGAAGAGGTG TCAGTCGAAA GAACTGAAAA ACAAACACCA
    CCGCTCCCAG AACCAAAGCC AGTATATGCA CAAGGAGGTC AGCCAGATGT GGATTTACCA
    GTCAGTCCGT CTGATGGTCC TTTACCCAAT TCAACCCATG AAGATGGAAT GCTTCGGCCA
    AGCATGAAAC TGGTAAAATT CAGAAAAGGA GATAGTGTGG GCCTGCGGCT CGCTGGTGGC
    AATGATGTTG GCATATTTGT AGCTGGTGTT CTAGAGGACA GCCCTGCAGC GAAAGAAGGA
    TTAGAGGAAG GAGACCAAAT TCTCAGGGTT AATAACGTAG ACTTCACAAA TATCATCAGA
    GAAGAAGCTG TCCTTTTTTT GCTTGATCTC CCTAAAGGCG AAGAAGTAAC CATTTTGGCA
    CAGAAGAAGA AAGATGTTTA TCGTCGCATT GTTGAGTCTG ATGTAGGGGA TTCTTTCTAT
    ATCAGAACTC ATTTTGAATA TGAAAAGGAA TCTCCTTATG GACTAAGCTT CAACAAAGGT
    GAAGTGTTTC GGGTTGTGGA CACGCTATAT AATGGCAAAC TGGGTTCGTG GCTGGCAATT
    CGAATTGGCA AGAATCATAA GGAAGTTGAA AGAGGTATCA TACCTAACAA AAACAGAGCT
    GAACAACTAG CTAGTGTACA GTACACGCTT CCAAAGACAG CAGGAGGAGA TCGAGCGGAT
    TTCTGGAGAT TCCGAGGTTT GCGTAGCTCA AAAAGAAATC TCCGAAAAAG TAGAGAAGAT
    CTTTCTGCTC AGCCTGTTCA GACAAAGTTC CCTGCCTATG AAAGAGTTGT TCTTCGGGAA
    GCTGGGTTTC TCAGACCTGT GACCATTTTT GGTCCAATAG CTGATGTTGC ACGAGAGAAA
    CTAGCCAGAG AAGAACCAGA TATCTTTCAA ATTGCAAAAA GTGAACCGAG AGATGCTGGT
    ACTGACCAAC GTAGTTCTGG CATTATTCGT CTTCATACGA TAAAACAGAT AATTGATAGA
    GACAAACACG CTTTATTAGA TGTCACTCCT AATGCAGTGG ACCGTCTGAA TTATGCACAG
    TGGTATCCTA TTGTAGTGTT TCTGAACCCT GATTCTAAGC AGGGTGTGAA GACCATGAGA
    ATGAGGCTGT GCCCAGAATC ACGAAAAAGT GCCAGGAAGC TATATGAAAG AGCTCACAAG
    CTACGCAAAA ATAATCACCA CCTCTTTACA ACTACCATTA ACTTGAATTC AATGAATGAA
    GGATGGTATG GAGCTCTAAA AGAAGCAATT CAGCAACAAC AAAACCAACT GGTGTGGGTT
    TCTGAAGGAA AGGCAGATGG TGCTACAAGT GATGACCTTG ATTTACATGA TGACCGCCTG
    TCTTACCTCT CAGCTCCTGG TAGTGAATAT TCTATGTACA GTACAGACAG CAGACATACA
    TCTGACTATG AAGACACAGA TACAGAAGGT GGTGCTTATA CTGATCAAGA ACTGGATGAA
    ACTCTGAACG ATGAGGTTGG AACTCCTCCT GAATCTGCTA TTACACGTTC TTCTGAACCC
    GTTAGGGAGG ATTCTTCTGG AATGCATCAT GAAACTCAAA CTTACACTAG TTACGCATCT
    CAAGCTCAGC CTCAGCCAAA TCTTAGAATA GATTCTTCAG GATTTAAAAC AACTGCTTCT
    CAGCCGAAAG CAGAAGCATC ACCTGCAGTC CCTTACCTTT CCCCGTCGCC TGAATCAAAC
    CCAGCAACCT CATCAACCTC TGCAGTAAAT GCTAATGTAA ACCTAACTAA TGTCAGACTA
    GAGGAGCCTA CTGCTGCTCC TTACAACTCT TATCAACCAC AAGCGGGCCC CTTAAGAACA
    TCAAGTACTG AGGGAGCTCA TGTAGTGCTA AGAGATCAAG AGCCATCATC CTTACCGCCG
    CATATAGATC CAGCAAAGGT ATACAGAAAG GATCCATATA TTAGTGAAGA AGCATCCAGA
    CAAAGCTATG TCTTAAAACA GCCAGCTGTT AATCACCCAG TACAAAGACA GGAAAGAGAC
    CCAAATCTGA TCTATGAATC TCAGACTCAG TATGCAGAAA AACAACCAAG TAGGGAGTAC
    GAGCAGTCAA CATACAGGTA TGATTCTACA AACTATGTAG ATCAATTTTC TCGTGGGTAT
    GACCCTCGTC TACATTATGA AGATCGTGCG CCTCCCTATG AGGAGCACTG GTCATATTAT
    GATGAAAAAC AGCCCTACAA TCAACCAAGA ACAGCTTATG AAAGCCAGCC TCCTAGAGAT
    CTTGATTCCA GACAAAATAC AGAAGAGAGC ACAGAACGCA GCTATTATCC GGCACAACCC
    CGATTTGAGG AACCTCCTCC GATGAGCTAC GATGGCAGAC CTCGCTATGA GCATGCACCC
    AAAAACTTCA GCTTGCCACA AGTGCGGTAT GAAGATCAAC ATCCTGTTGG CTATGACACA
    CATGCGCATG GTAGATATAA ACAAGAAGCT CAGCCATACC AGTCAGCCAT ATCTCGATCT
    CCTGAACCCA AGCAGTACTT TGATCCACAT GTGAGGGGCT ATGAGCAAGG TCCTCCTCAA
    GCTTACAATG CAAAAGCTGG ACAGTTTGAG CCTTCTCATA GCACTTCAGG TGTTTCTCTT
    CCTCCTCCCG CTTCTTCACA AACAAAACCA GAAGTTTTGC CTTCCAACAG TAAACCACTG
    CCTACACCAC CATCTCTAGC AGAAGAAGAG GAGGATCCAG CCATGAAACC ACAGTCTGTG
    CGAAGTAGAG TTAAGATATT TGAACGAAGA AGATCGCCAT CTCTGGAAAA AATGAAGGAC
    CCCAGTGACA CGTCAGTTGT CAAACCTCCA GAGCTAGCAC CGAAGCCTAC GCTCCCAGCC
    ATGAGTGGTC CAAAACCAAC TTCTCAAAGC CAGTATGAAC ATGATAAAAC AACTTACAGG
    GCCCCAGAAC CACAGAGACC TCAAGTGAAG CCACCTGAAG ATATCGTGCG TTCAAATCAT
    TATGATCCCG AAGAAGATGA AGAGTATTAC AGAAAGCAGC TCTCATACTT TGATCGCAGG
    AATTTTGAAA ATAAGCCTTC TGCACAGGTT CCTGCCAGCC ATCATTCTGA CTCCACTAAA
    CCAATACATT CACAAAATCA GCTGAATTTC TCCAATTATT CTAAGGGGAA GCCAACTGAT
    GCTGAACCAA TGGATAGATC TGTTGGTGAC AAGCGCTATG AGCCAATCCC TCAAGTTACA
    ACTCCTCCTC CTGCTCCTTC AGTACAGTAT ACACAACCTC AGTCAATTAA TAGTCCTGTC
    CTGTCTCTCC CAGCGCATCA CAAGCCTGCA CTTTCTGAAG TTAACTCTGT GTCTGACCCT
    CCTCCACCTC AGAATAAGCC AGCAATTTTC AGATCCTCTA GAGAGGACAC TGTGCAGTCT
    ACTTTTTATC CTCAGAAAAG CTTCCCTGAC AAAGGTCCTG TTAATGGAAC TGAACAGGTT
    CAGAAAACAG TCACTCCTTC TTACAACCGT TTTACAACAA AACCTTACAC TAGTGCTGCG
    AGGCCCTTTG AGCGCAAGTT TGAAAGTCCT AAATTCAACC ATAATCTCTT GCCAAATGAA
    GCTCAACATA AACCAGAGTT GCCATCAAAA TCTCCAAATT CTCCTCAACC AATTTTGAAA
    GCACACAGCT CATCACAGCC TCCTGAGTTT GACAGTGGAA TGGACACCTT CACTGTACAG
    GTTGACAAGC CTAAATATCA ACCAAATAAT GTAAATGCTG TGCCTAAAGC CATTCCTGTA
    AGCCCTTCAG CACTAGAGGA TGAGGAGGAA GAAGACGGAC ACACTGTTGT AGCCACAGCA
    AGAGGTGTAT TCAACAGCAA TGGTGGCGTA TTGAGCTCCA TAGAGACTGG AGTTAGTATT
    ATTATACCAC AAGGAGCCAT TCCAGAGGGA ATAGAGCAAG AAATATATTT TAAAGTCTGC
    AGAGACAACA GTATACTTCC ACCTTTGGAC AAAGAGAAAG GTGAAACACT TCTTAGCCCC
    TTGGTAATGT GTGGGCCTCA CGGACTAAAA TTCCTGAAGC CAGTGGAGCT GCGCCTGCCA
    CACTGTGCGT CTATGACCCC TGATGGTTGG TCTTTTGCTC TAAAATCCTC TGACTCCTCG
    TCGGGTGACC CCAAAACCTG GCAGAACAAG TCTCTTCCTG GAGATCCAAA CTATCTTGTT
    GGAGCAAACT GTGTCTCAGT CCTAATTGAC CACTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.