tjp1b gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_021470725.1	XP_021326400.1	tight junction protein ZO-1 isoform X3
XM_021470728.1	XP_021326403.1	tight junction protein ZO-1 isoform X7
XM_017354171.1	XP_017209660.1	tight junction protein ZO-1 isoform X7
XM_017354175.1	XP_017209664.1	tight junction protein ZO-1 isoform X10
XM_009298130.2	XP_009296405.1	tight junction protein ZO-1 isoform X1
XM_017354169.1	XP_017209658.1	tight junction protein ZO-1 isoform X4
XM_017354172.1	XP_017209661.1	tight junction protein ZO-1 isoform X8
XM_009298132.2	XP_009296407.1	tight junction protein ZO-1 isoform X2
XM_009298141.2	XP_009296416.1	tight junction protein ZO-1 isoform X15
XM_021470724.1	XP_021326399.1	tight junction protein ZO-1 isoform X2
XM_021470727.1	XP_021326402.1	tight junction protein ZO-1 isoform X5
XM_009298135.3	XP_009296410.1	tight junction protein ZO-1 isoform X13
XM_009298137.3	XP_009296412.1	tight junction protein ZO-1 isoform X16
XM_021470737.1	XP_021326412.1	tight junction protein ZO-1 isoform X19
XM_017354168.1	XP_017209657.1	tight junction protein ZO-1 isoform X3
XM_017354173.1	XP_017209662.1	tight junction protein ZO-1 isoform X9
XM_017354176.1	XP_017209665.1	tight junction protein ZO-1 isoform X12
XM_017354177.1	XP_017209666.1	tight junction protein ZO-1 isoform X13
XM_009298135.2	XP_009296410.1	tight junction protein ZO-1 isoform X6
XM_021470726.1	XP_021326401.1	tight junction protein ZO-1 isoform X4
XM_009298130.3	XP_009296405.1	tight junction protein ZO-1 isoform X6
XM_009298141.3	XP_009296416.1	tight junction protein ZO-1 isoform X20
XM_009298137.2	XP_009296412.1	tight junction protein ZO-1 isoform X11
XM_021470731.1	XP_021326406.1	tight junction protein ZO-1 isoform X10
XM_017354170.2	XP_017209659.1	tight junction protein ZO-1 isoform X12
XM_021470733.1	XP_021326408.1	tight junction protein ZO-1 isoform X15
XM_021470734.1	XP_021326409.1	tight junction protein ZO-1 isoform X17
XM_021470736.1	XP_021326411.1	tight junction protein ZO-1 isoform X18
XM_017354170.1	XP_017209659.1	tight junction protein ZO-1 isoform X5
XM_017354178.1	XP_017209667.1	tight junction protein ZO-1 isoform X14
XM_021470723.1	XP_021326398.1	tight junction protein ZO-1 isoform X1
XM_009298132.3	XP_009296407.1	tight junction protein ZO-1 isoform X8
XM_021470730.1	XP_021326405.1	tight junction protein ZO-1 isoform X9
XM_021470732.1	XP_021326407.1	tight junction protein ZO-1 isoform X11
XM_017354172.2	XP_017209661.1	tight junction protein ZO-1 isoform X14

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa141490	XM_021470724.1 Latest version!	Danio rerio tight junction protein 1b (tjp1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa141491	XM_021470731.1 Latest version!	Danio rerio tight junction protein 1b (tjp1b), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa44616	XM_021470723.1 Latest version!	Danio rerio tight junction protein 1b (tjp1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa44617	XM_021470725.1 Latest version!	Danio rerio tight junction protein 1b (tjp1b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa44618	XM_021470726.1 Latest version!	Danio rerio tight junction protein 1b (tjp1b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa44619	XM_021470727.1 Latest version!	Danio rerio tight junction protein 1b (tjp1b), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa44620	XM_009298130.3 Latest version!	Danio rerio tight junction protein 1b (tjp1b), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa44621	XM_021470728.1 Latest version!	Danio rerio tight junction protein 1b (tjp1b), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa44622	XM_009298132.3 Latest version!	Danio rerio tight junction protein 1b (tjp1b), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa44623	XM_021470730.1 Latest version!	Danio rerio tight junction protein 1b (tjp1b), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa44624	XM_021470732.1 Latest version!	Danio rerio tight junction protein 1b (tjp1b), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa44625	XM_009298135.3 Latest version!	Danio rerio tight junction protein 1b (tjp1b), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa44626	XM_021470733.1 Latest version!	Danio rerio tight junction protein 1b (tjp1b), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
ODa44627	XM_009298137.3 Latest version!	Danio rerio tight junction protein 1b (tjp1b), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
ODa44628	XM_021470734.1 Latest version!	Danio rerio tight junction protein 1b (tjp1b), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
ODa44629	XM_021470736.1 Latest version!	Danio rerio tight junction protein 1b (tjp1b), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
ODa44630	XM_021470737.1 Latest version!	Danio rerio tight junction protein 1b (tjp1b), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
ODa44631	XM_009298141.3 Latest version!	Danio rerio tight junction protein 1b (tjp1b), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
ODa57879	XM_017354168.1 Latest version!	Danio rerio tight junction protein 1b (tjp1b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa57880	XM_017354169.1 Latest version!	Danio rerio tight junction protein 1b (tjp1b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa57881	XM_017354170.2 Latest version!	Danio rerio tight junction protein 1b (tjp1b), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa57882	XM_017354171.1 Latest version!	Danio rerio tight junction protein 1b (tjp1b), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa57883	XM_017354172.2 Latest version!	Danio rerio tight junction protein 1b (tjp1b), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa57884	XM_017354173.1 Latest version!	Danio rerio tight junction protein 1b (tjp1b), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa57885	XM_017354175.1 Latest version!	Danio rerio tight junction protein 1b (tjp1b), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
ODa57886	XM_017354176.1 Latest version!	Danio rerio tight junction protein 1b (tjp1b), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
ODa57887	XM_017354177.1 Latest version!	Danio rerio tight junction protein 1b (tjp1b), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
ODa57888	XM_017354178.1 Latest version!	Danio rerio tight junction protein 1b (tjp1b), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
View Old Accession Versions >>
ODa44625	XM_009298135.2	Danio rerio tight junction protein 1b (tjp1b), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa44620	XM_009298130.2	Danio rerio tight junction protein 1b (tjp1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa44631	XM_009298141.2	Danio rerio tight junction protein 1b (tjp1b), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
ODa44627	XM_009298137.2	Danio rerio tight junction protein 1b (tjp1b), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
ODa57881	XM_017354170.1	Danio rerio tight junction protein 1b (tjp1b), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa44622	XM_009298132.2	Danio rerio tight junction protein 1b (tjp1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa57883	XM_017354172.1	Danio rerio tight junction protein 1b (tjp1b), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
Hide Old Accession Versions >>

tjp1b ( XM_021470724.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326399.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa141490
Clone ID Related Accession (Same CDS sequence)	XM_021470724.1
Accession Version	XM_021470724.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6129bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tight junction protein ZO-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
TCCCGCAAGA ATGAAGCATG TATGGCGTCA GGAAGTTCTC AGAAGGTGAC ATCAGAATAC 
GGGCTTCGCC TGGCCAGCCA CATCTTCGTG AAGGACATTT CCCCCGAGAG CCTGGCAGCC 
CGAGACGGAA ACATCCAAGA GGGAGACGTG GTGCTTAAGA TTAATGGCAC GGTAACAGAG 
AATTTGTCCC TGATCGATGC TAAGAAGCTG ATCGAGAGGT CAAAGGGAAA GCTGAAGATG 
GTGGTGCAGA GAGACGAGCG GGCGACGCTG CTGAACATCC CCGATCTGGA CGACAGCATA 
CCTTCTGCTA ATGCCTCGGA CAGAGATGAT ATTTCAGACA TCCACTCCGT GGCCTCTGAT 
CATTCCAACC GATCCCACGA CCGGAAGAGG AGCAGCCGCT CTCGTTCTCC AGACCGCCGC 
TCCGAACCCT CAGACCACTC CAGACACTCT CCACCACAGA TCAGCAACGG CAGGGCCACA 
GGGCCGACGG CTCAGAGAAC GCGGCCAGTT CACAGGAGCC GTGATGAAGA CAGGATCTCC 
AAGGCCGGGC CGCTTCCTCT ACCTGCTAAG ATGGTGGAGG AGATGCCGAA AGAGCAGAGC 
GCAGCCAGAG AAACAGAGAA ACAGCTCCCC CCTCTGCCAG AGCCCAAACC TGTGTACGCT 
CAGCCGGGCC AGCCGGACGT GGACCTGCCT GTTAGTCCGG CAGATGCTCC TGTGCCCAGC 
GTCACACATG ATGACAGCAT CTTACGGCCG AGCATGAAGC TGGTGAAGTT CAAGAAGGGC 
GAGAGCGTGG GCTTGAGGCT CGCTGGGGGG AACGACGTTG GGATATTTGT GGCGGGAGTT 
CTGGAGGACA GTCCTGCTGC TAAAGAGGGC CTGGAAGAGG GAGACCAGAT TCTCAGGGTA 
AATAATGTGG ATTTTGCAAA TATCATCCGA GAGGAGGCAG TGCTGTTCCT GCTGGATCTG 
CCCAAAGGAG AGGAGGTCAC CATCCTGGCC CAGAAGAAGA AAGACGTGTA CCGCCGCATT 
GTAGAGTCTG ACGTGGGAGA CTCGTTCTAC ATCAGGACTC ATTTTGAATA TGAGAAGGAG 
TCGCCCTATG GCCTGAGCTT CAATAAGGGG GAGGTCTTCA GAGTCGTCGA CACCCTTTAC 
AATGGCAAAC TGGGCTCCTG GCTCGCCATC CGCATCGGCA AAAACCACCA GGAGGTGGAG 
CGGGGCATCA TCCCCAACAA GAACAGGGCT GAGCAGTTAT CAAGTGTCCA GTACACTCTC 
CCAAAGACTC CAGGAGGGGA TCGCGCCGAC TTCTGGAGAT TCAGAGGCCT CCGCAGCTCC 
AAACGAAACC TGAGGAAGAG CAGAGAAGAC CTGTCTGCTC AGCCGGTCCA AACCAAATTC 
CCTGCTTATG AAAGAGTGGT GCTTAGAGAA GCTGGTTTCT TGAGGCCAGT GGTCATTTTC 
GGACCGATCG CGGATGTGGC TAGAGAGAAG CTGTCCAGAG AGGAGACTGA CCTCTTCGAA 
CTTGCAAAGA GTGAACCCAG AGATGCGGGC ACAGACCAGC GCAGCTCCGG AATCATCCGT 
CTCCATACCA TTAAACAGAT CATTGACCGA GACAAGCACG CGGTGCTGGA CATCACCCCT 
AATGCGGTGG ACCGTCTGAA CTACGCTCAG TGGTACCCCA TCGTGGTGTT TCTGAATCCA 
GACAGCAAAC AGGGCGTCAA GAACATGAGG ACCAGGCTCT GTCCCGAGTC CAGGAAGAGC 
GCCCGAAAGC TCTACGAGCG TGCTCTCAAA CTGAGGAAGA ACAACCATCA CCTTTTCACC 
ACCACTATTA ACTTGAACAA CATGAATGAC GGGTGGTACG GTGCTTTGAA AGAGAGCATC 
CAGCAGCAGC AGAACCAGTT GGTCTGGGTC TCAGAGGGCA AGGCCGACGG CGCTCCTGAC 
GATGACCTTG ACATCCATGA CGACCGCCTC TCCTACCTCT CGGCTCCGGG CAGCGAGTAC 
AGCATGTACA GCACCGACAG CCGCCACACG TCCGACTATG AGGACACCGA CACGGAGGGT 
GGTGCCTACA CCGACCAGGA GCTGGACGAG ACCATGAACG ATGAGGTGGG CCTGCCTAGC 
GAGCCTGCCA TCACACGCTC ATCTGAGCCT GTGCGAGAGG ACCCGCCAGT CATTCAGGAC 
GCAGCTGGAT ACCCCAGCTA CCAGCATGCG GTACCGCAAC CTGAAGCGGT GAGCCGCATT 
GACCCGGCCG GGTTTAAGAT GGCAGCGCCG CAGCAGCAAG CTGAGGCTGC TCTGGCCTCG 
CCGCCCCCTC CCTCTGCAGC GGTCCAGCCC ACCGCAACAC TAGCGGGTAC GAACCCCGAG 
GAGCCGCCTG GCACCCCTCA GGCCGACTCC CTTAACAGCC CCGTCCCTGT TCCCGACCCC 
CAGCCCGAAC CTGAGCTCGC TCAGCCCCCA ACACACGACC CCCACCAGTC GCTTCCTCCT 
GGCCCAGATC CAAAGATGTA TAAAAAGGAT CTGTACAGCA TGGACGAGCC GGTGCGAGTG 
ACCCACGGCG TGAAGCCTCC CCCTCCCCAG CAGCAGCAGC AGCCTTTAGG CCCCCCAACC 
TCGCTCTCCT ACAGCCACCA GCCTGTCTAC CAGGACCAAC AGCCATACCG CCAGTACGAC 
CACCCGCCTT ATGGCTATGA CGGTGGCGGC TACGCACAAC CAAAGCCTCA CAACTACGAC 
CCGCACCTGC ACTACGACAA CCGTGTGCCT CACTATAACG AGCAGTGGCC CACGTACGAC 
CAGCAGCAGC AGCAGCAGAC TTCCCCCCAG CCGCCACCTG CGTCCGGCTT CCCTCAGGGC 
CACCAGCCTC CGCCTCCACC ACCGATGGGA TATGAGCCAC GCTCCCCATA CGAGGACGGA 
CCTAACAGGG ATTTCAGCCC TCCTCAGTCC CAGTATGACG GTGTGTCCCC AATGGGCTAC 
GATCACCGGC CGCGGCACCC CAAACCTGCA CCGGTCCGAT ATGAAGAGCC TCCACCTCCA 
CCTCCTCCGG TGTCCTACGA TGCACGGTCA CCTTTCGAAC CCGAGCCGCA TGCTTTCCCA 
GGCAACACAC ATCGCTCGCC AGACCCACCG AAACAGTATT ATGGAGACGC CGCTATGAGG 
CCCTTGTACA ACCCTGGACC TCCGAACAGA GTGTACAAAC ATGAGCCGGT GAACTCTGAG 
CCTCCTGTAC CGCCTCCCAA ACCTGAATCT GTCCTGTCTC CAGGAGAACC GCCCCATTCT 
GCAGCTTCCA AACCATTGCC GCCTCCTCCC AGAGATGATG ACGATGATGA TGACGATGAT 
GATGACCCTG CCATGAAGCC CCAGTCTGTG CTCAACAGGG TCAAAATGTT CGAAAACAAA 
CGCTCTGTGT CGGTAGACCG AGCCAAGGAC GCACCTGACG TCACTGGAAT TAGGCCCACA 
GATCTCCCCA AACCTGCGAG CACCCCTGGA CCTGTGCTCA AAGCCAACTC TCTCAGCAAC 
CTGGAGCAGG ACAAACCCTC CTACAGAGCT CCAGAGCCGC AGAAACCTCA GACGCGTGTT 
GGGGACGATG TGGTTCGCTC CAACCACTAC GACCCTGATG ATGATGAAGA GTACTACAGA 
AAACAGCTCT CATTTTTCGA CCGCCGCAGT TTCGACAGTA AGGTCATGAA TCCACCCAGC 
GCTGGAGTAA ACCGCTTCCA TGAGCCGCCC AAACCACCCC AGACACAGAT CGCTTATCCT 
TTCGCTAGGA CGGAGTCTGT GGAGAAGGTG AGTCCTGTGG ACAAGAGATA CGAGCCTCTG 
CCGCCAGTCA ACCCTTCACC TGCTCCATAC AGCCAGAACA CACCTGTCGC TCCGCCCACA 
TCCCTGCCCA AACTCACCAA CATCGAGGTG AACTCTCTGC CAGATCCTCA CAGCTCTCCC 
AAAGCCAAAC CGGACCTGTC TGCTCTGAGA GCTCCGACCC GGGACGAGCC CATCCAGAGC 
AGCTACTTGC CCCCCAAGTC CACCATCAAT GGCATGGATG CTCCTCCCAA AACACTCGGC 
GTGCCGACCA GCTACAACCG CTACGTCCCC AAGCCTTACA CCAGCTCGGC GCGACCCTTC 
GAGCGCAAGT TCGAGAGTCC GAAATTCAAC CACAACCTGC TTCCCAACGA CACGCAGTCC 
AAACCCAGTG TCAACAACAA CCTGAAGCCT CAGATCTCCC CACAACCCCA GGACACGGAC 
AGCGGCGTCG ACACCTTCAC TCGCACCATG GACAACAGGC CAAAATATCA GCACAACAAC 
ATCAACGCCA TTCCCAAAGC CGTCCCAGTC AGCCCCAGTG CACTGGATGA TGATGAAGAA 
GATGAGGGAC ACACAGTTGT GGCCACGGCG CGGGGCATCT TTAACTGTAA TGGCGGGGTG 
TTGAGCTCTA TAGAGACGGG CGTCAGCATC ATTATCCCAC AGGGAGCCAT TCCTGATAGT 
GTGGAGCAGG AGATCTACTT CAAAGTGTGC CGGGACAACA GCATCCTGCC TCCTCTGGAT 
AAGGAGAAAG GTGAAACTCT GCTGAGTCCG CTGGTGATGT GTGGTCCTCA CGGCCTGAAG 
TTCCTGAAGC CTGTGGAGCT GCGCTTGCCA CACTGTGCGT CTATGACCCC TGATGGTTGG 
TCTTTTGCTC TAAAATCCTC CGACTCCTCG TCGGGTGACC CCAAATGCTG GCAAAACAAG 
TGTCTACCAG GAGACCCCAA TTACCTGGTG GGCGCAAACT GTGTGTCTGT GCTGATCGAC 
CACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326399.1
CDS	110..6238
Translation	MLLSVFGGPVEEVLAGLLKLKMFGSGSKKWFIKKNKRQRTKVQRKPASGRRNKRVSRVLKRHKEVIVKQLDVSKVLPTLVYERVFTLGEYKEILGQDSSGKRAELFLDLLSQKEPAAFSAFCGVLEEVCPHLLTCFLLDGTDGSAGENKKQGQTKPAHSQQNALSKGQDTVCFPPMYTDPLFDTRVFPKHDKDKYGPAVYYPKFNAYIPNPDKVSVLDESEGSGHGKPSLRRIKGRIHRSKSLDSIDLLDSNSAAMDETVIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGLLQENDRVVMVNAVSMDNVDHAYAVQQLRKSGKNAKITIRRKRKVQIPSGRPGERETMSEHDEDDDSYEDEIYESRSARSGPSAYSGGGGGGGGGGASGRRSGRGDRLSGRRERDRERSGSRERSLSPRSDRHSVSSNLPPRPAKVTLVKSRKNEACMASGSSQKVTSEYGLRLASHIFVKDISPESLAARDGNIQEGDVVLKINGTVTENLSLIDAKKLIERSKGKLKMVVQRDERATLLNIPDLDDSIPSANASDRDDISDIHSVASDHSNRSHDRKRSSRSRSPDRRSEPSDHSRHSPPQISNGRATGPTAQRTRPVHRSRDEDRISKAGPLPLPAKMVEEMPKEQSAARETEKQLPPLPEPKPVYAQPGQPDVDLPVSPADAPVPSVTHDDSILRPSMKLVKFKKGESVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFANIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHQEVERGIIPNKNRAEQLSSVQYTLPKTPGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVVIFGPIADVAREKLSREETDLFELAKSEPRDAGTDQRSSGIIRLHTIKQIIDRDKHAVLDITPNAVDRLNYAQWYPIVVFLNPDSKQGVKNMRTRLCPESRKSARKLYERALKLRKNNHHLFTTTINLNNMNDGWYGALKESIQQQQNQLVWVSEGKADGAPDDDLDIHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETMNDEVGLPSEPAITRSSEPVREDPPVIQDAAGYPSYQHAVPQPEAVSRIDPAGFKMAAPQQQAEAALASPPPPSAAVQPTATLAGTNPEEPPGTPQADSLNSPVPVPDPQPEPELAQPPTHDPHQSLPPGPDPKMYKKDLYSMDEPVRVTHGVKPPPPQQQQQPLGPPTSLSYSHQPVYQDQQPYRQYDHPPYGYDGGGYAQPKPHNYDPHLHYDNRVPHYNEQWPTYDQQQQQQTSPQPPPASGFPQGHQPPPPPPMGYEPRSPYEDGPNRDFSPPQSQYDGVSPMGYDHRPRHPKPAPVRYEEPPPPPPPVSYDARSPFEPEPHAFPGNTHRSPDPPKQYYGDAAMRPLYNPGPPNRVYKHEPVNSEPPVPPPKPESVLSPGEPPHSAASKPLPPPPRDDDDDDDDDDDPAMKPQSVLNRVKMFENKRSVSVDRAKDAPDVTGIRPTDLPKPASTPGPVLKANSLSNLEQDKPSYRAPEPQKPQTRVGDDVVRSNHYDPDDDEEYYRKQLSFFDRRSFDSKVMNPPSAGVNRFHEPPKPPQTQIAYPFARTESVEKVSPVDKRYEPLPPVNPSPAPYSQNTPVAPPTSLPKLTNIEVNSLPDPHSSPKAKPDLSALRAPTRDEPIQSSYLPPKSTINGMDAPPKTLGVPTSYNRYVPKPYTSSARPFERKFESPKFNHNLLPNDTQSKPSVNNNLKPQISPQPQDTDSGVDTFTRTMDNRPKYQHNNINAIPKAVPVSPSALDDDEEDEGHTVVATARGIFNCNGGVLSSIETGVSIIIPQGAIPDSVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKCWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_021470724.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tight junction protein 1b (tjp1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470724.1

ORF Insert Sequence:

ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
TCCCGCAAGA ATGAAGCATG TATGGCGTCA GGAAGTTCTC AGAAGGTGAC ATCAGAATAC 
GGGCTTCGCC TGGCCAGCCA CATCTTCGTG AAGGACATTT CCCCCGAGAG CCTGGCAGCC 
CGAGACGGAA ACATCCAAGA GGGAGACGTG GTGCTTAAGA TTAATGGCAC GGTAACAGAG 
AATTTGTCCC TGATCGATGC TAAGAAGCTG ATCGAGAGGT CAAAGGGAAA GCTGAAGATG 
GTGGTGCAGA GAGACGAGCG GGCGACGCTG CTGAACATCC CCGATCTGGA CGACAGCATA 
CCTTCTGCTA ATGCCTCGGA CAGAGATGAT ATTTCAGACA TCCACTCCGT GGCCTCTGAT 
CATTCCAACC GATCCCACGA CCGGAAGAGG AGCAGCCGCT CTCGTTCTCC AGACCGCCGC 
TCCGAACCCT CAGACCACTC CAGACACTCT CCACCACAGA TCAGCAACGG CAGGGCCACA 
GGGCCGACGG CTCAGAGAAC GCGGCCAGTT CACAGGAGCC GTGATGAAGA CAGGATCTCC 
AAGGCCGGGC CGCTTCCTCT ACCTGCTAAG ATGGTGGAGG AGATGCCGAA AGAGCAGAGC 
GCAGCCAGAG AAACAGAGAA ACAGCTCCCC CCTCTGCCAG AGCCCAAACC TGTGTACGCT 
CAGCCGGGCC AGCCGGACGT GGACCTGCCT GTTAGTCCGG CAGATGCTCC TGTGCCCAGC 
GTCACACATG ATGACAGCAT CTTACGGCCG AGCATGAAGC TGGTGAAGTT CAAGAAGGGC 
GAGAGCGTGG GCTTGAGGCT CGCTGGGGGG AACGACGTTG GGATATTTGT GGCGGGAGTT 
CTGGAGGACA GTCCTGCTGC TAAAGAGGGC CTGGAAGAGG GAGACCAGAT TCTCAGGGTA 
AATAATGTGG ATTTTGCAAA TATCATCCGA GAGGAGGCAG TGCTGTTCCT GCTGGATCTG 
CCCAAAGGAG AGGAGGTCAC CATCCTGGCC CAGAAGAAGA AAGACGTGTA CCGCCGCATT 
GTAGAGTCTG ACGTGGGAGA CTCGTTCTAC ATCAGGACTC ATTTTGAATA TGAGAAGGAG 
TCGCCCTATG GCCTGAGCTT CAATAAGGGG GAGGTCTTCA GAGTCGTCGA CACCCTTTAC 
AATGGCAAAC TGGGCTCCTG GCTCGCCATC CGCATCGGCA AAAACCACCA GGAGGTGGAG 
CGGGGCATCA TCCCCAACAA GAACAGGGCT GAGCAGTTAT CAAGTGTCCA GTACACTCTC 
CCAAAGACTC CAGGAGGGGA TCGCGCCGAC TTCTGGAGAT TCAGAGGCCT CCGCAGCTCC 
AAACGAAACC TGAGGAAGAG CAGAGAAGAC CTGTCTGCTC AGCCGGTCCA AACCAAATTC 
CCTGCTTATG AAAGAGTGGT GCTTAGAGAA GCTGGTTTCT TGAGGCCAGT GGTCATTTTC 
GGACCGATCG CGGATGTGGC TAGAGAGAAG CTGTCCAGAG AGGAGACTGA CCTCTTCGAA 
CTTGCAAAGA GTGAACCCAG AGATGCGGGC ACAGACCAGC GCAGCTCCGG AATCATCCGT 
CTCCATACCA TTAAACAGAT CATTGACCGA GACAAGCACG CGGTGCTGGA CATCACCCCT 
AATGCGGTGG ACCGTCTGAA CTACGCTCAG TGGTACCCCA TCGTGGTGTT TCTGAATCCA 
GACAGCAAAC AGGGCGTCAA GAACATGAGG ACCAGGCTCT GTCCCGAGTC CAGGAAGAGC 
GCCCGAAAGC TCTACGAGCG TGCTCTCAAA CTGAGGAAGA ACAACCATCA CCTTTTCACC 
ACCACTATTA ACTTGAACAA CATGAATGAC GGGTGGTACG GTGCTTTGAA AGAGAGCATC 
CAGCAGCAGC AGAACCAGTT GGTCTGGGTC TCAGAGGGCA AGGCCGACGG CGCTCCTGAC 
GATGACCTTG ACATCCATGA CGACCGCCTC TCCTACCTCT CGGCTCCGGG CAGCGAGTAC 
AGCATGTACA GCACCGACAG CCGCCACACG TCCGACTATG AGGACACCGA CACGGAGGGT 
GGTGCCTACA CCGACCAGGA GCTGGACGAG ACCATGAACG ATGAGGTGGG CCTGCCTAGC 
GAGCCTGCCA TCACACGCTC ATCTGAGCCT GTGCGAGAGG ACCCGCCAGT CATTCAGGAC 
GCAGCTGGAT ACCCCAGCTA CCAGCATGCG GTACCGCAAC CTGAAGCGGT GAGCCGCATT 
GACCCGGCCG GGTTTAAGAT GGCAGCGCCG CAGCAGCAAG CTGAGGCTGC TCTGGCCTCG 
CCGCCCCCTC CCTCTGCAGC GGTCCAGCCC ACCGCAACAC TAGCGGGTAC GAACCCCGAG 
GAGCCGCCTG GCACCCCTCA GGCCGACTCC CTTAACAGCC CCGTCCCTGT TCCCGACCCC 
CAGCCCGAAC CTGAGCTCGC TCAGCCCCCA ACACACGACC CCCACCAGTC GCTTCCTCCT 
GGCCCAGATC CAAAGATGTA TAAAAAGGAT CTGTACAGCA TGGACGAGCC GGTGCGAGTG 
ACCCACGGCG TGAAGCCTCC CCCTCCCCAG CAGCAGCAGC AGCCTTTAGG CCCCCCAACC 
TCGCTCTCCT ACAGCCACCA GCCTGTCTAC CAGGACCAAC AGCCATACCG CCAGTACGAC 
CACCCGCCTT ATGGCTATGA CGGTGGCGGC TACGCACAAC CAAAGCCTCA CAACTACGAC 
CCGCACCTGC ACTACGACAA CCGTGTGCCT CACTATAACG AGCAGTGGCC CACGTACGAC 
CAGCAGCAGC AGCAGCAGAC TTCCCCCCAG CCGCCACCTG CGTCCGGCTT CCCTCAGGGC 
CACCAGCCTC CGCCTCCACC ACCGATGGGA TATGAGCCAC GCTCCCCATA CGAGGACGGA 
CCTAACAGGG ATTTCAGCCC TCCTCAGTCC CAGTATGACG GTGTGTCCCC AATGGGCTAC 
GATCACCGGC CGCGGCACCC CAAACCTGCA CCGGTCCGAT ATGAAGAGCC TCCACCTCCA 
CCTCCTCCGG TGTCCTACGA TGCACGGTCA CCTTTCGAAC CCGAGCCGCA TGCTTTCCCA 
GGCAACACAC ATCGCTCGCC AGACCCACCG AAACAGTATT ATGGAGACGC CGCTATGAGG 
CCCTTGTACA ACCCTGGACC TCCGAACAGA GTGTACAAAC ATGAGCCGGT GAACTCTGAG 
CCTCCTGTAC CGCCTCCCAA ACCTGAATCT GTCCTGTCTC CAGGAGAACC GCCCCATTCT 
GCAGCTTCCA AACCATTGCC GCCTCCTCCC AGAGATGATG ACGATGATGA TGACGATGAT 
GATGACCCTG CCATGAAGCC CCAGTCTGTG CTCAACAGGG TCAAAATGTT CGAAAACAAA 
CGCTCTGTGT CGGTAGACCG AGCCAAGGAC GCACCTGACG TCACTGGAAT TAGGCCCACA 
GATCTCCCCA AACCTGCGAG CACCCCTGGA CCTGTGCTCA AAGCCAACTC TCTCAGCAAC 
CTGGAGCAGG ACAAACCCTC CTACAGAGCT CCAGAGCCGC AGAAACCTCA GACGCGTGTT 
GGGGACGATG TGGTTCGCTC CAACCACTAC GACCCTGATG ATGATGAAGA GTACTACAGA 
AAACAGCTCT CATTTTTCGA CCGCCGCAGT TTCGACAGTA AGGTCATGAA TCCACCCAGC 
GCTGGAGTAA ACCGCTTCCA TGAGCCGCCC AAACCACCCC AGACACAGAT CGCTTATCCT 
TTCGCTAGGA CGGAGTCTGT GGAGAAGGTG AGTCCTGTGG ACAAGAGATA CGAGCCTCTG 
CCGCCAGTCA ACCCTTCACC TGCTCCATAC AGCCAGAACA CACCTGTCGC TCCGCCCACA 
TCCCTGCCCA AACTCACCAA CATCGAGGTG AACTCTCTGC CAGATCCTCA CAGCTCTCCC 
AAAGCCAAAC CGGACCTGTC TGCTCTGAGA GCTCCGACCC GGGACGAGCC CATCCAGAGC 
AGCTACTTGC CCCCCAAGTC CACCATCAAT GGCATGGATG CTCCTCCCAA AACACTCGGC 
GTGCCGACCA GCTACAACCG CTACGTCCCC AAGCCTTACA CCAGCTCGGC GCGACCCTTC 
GAGCGCAAGT TCGAGAGTCC GAAATTCAAC CACAACCTGC TTCCCAACGA CACGCAGTCC 
AAACCCAGTG TCAACAACAA CCTGAAGCCT CAGATCTCCC CACAACCCCA GGACACGGAC 
AGCGGCGTCG ACACCTTCAC TCGCACCATG GACAACAGGC CAAAATATCA GCACAACAAC 
ATCAACGCCA TTCCCAAAGC CGTCCCAGTC AGCCCCAGTG CACTGGATGA TGATGAAGAA 
GATGAGGGAC ACACAGTTGT GGCCACGGCG CGGGGCATCT TTAACTGTAA TGGCGGGGTG 
TTGAGCTCTA TAGAGACGGG CGTCAGCATC ATTATCCCAC AGGGAGCCAT TCCTGATAGT 
GTGGAGCAGG AGATCTACTT CAAAGTGTGC CGGGACAACA GCATCCTGCC TCCTCTGGAT 
AAGGAGAAAG GTGAAACTCT GCTGAGTCCG CTGGTGATGT GTGGTCCTCA CGGCCTGAAG 
TTCCTGAAGC CTGTGGAGCT GCGCTTGCCA CACTGTGCGT CTATGACCCC TGATGGTTGG 
TCTTTTGCTC TAAAATCCTC CGACTCCTCG TCGGGTGACC CCAAATGCTG GCAAAACAAG 
TGTCTACCAG GAGACCCCAA TTACCTGGTG GGCGCAAACT GTGTGTCTGT GCTGATCGAC 
CACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp1b ( XM_021470731.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326406.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X10, mRNA.

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CloneID	ODa141491
Clone ID Related Accession (Same CDS sequence)	XM_021470731.1
Accession Version	XM_021470731.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6078bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tight junction protein ZO-1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
TCCCGCAAGA ATGAAGCATG TATGGCGTCA GGAAGTTCTC AGAAGGTGAC ATCAGCAGAA 
TACGGGCTTC GCCTGGCCAG CCACATCTTC GTGAAGGACA TTTCCCCCGA GAGCCTGGCA 
GCCCGAGACG GAAACATCCA AGAGGGAGAC GTGGTGCTTA AGAAGCTGAT CGAGAGGTCA 
AAGGGAAAGC TGAAGATGGT GGTGCAGAGA GACGAGCGGG CGACGCTGCT GAACATCCCC 
GATCTGGACG ACAGCATACC TTCTGCTAAT GCCTCGGACA GAGATGACAT CCACTCCGTG 
GCCTCTGATC ATTCCAACCG ATCCCACGAC CGGAAGAGGA GCAGCCGCTC TCGTTCTCCA 
GACCGCCGCT CCGAACCCTC AGACCACTCC AGACACTCTC CACCACAGAT CAGCAACGGC 
AGGGCCACAG GGCCGACGGC TCAGAGAACG CGGCCAGTTC ACAGGAGCCG TGATGAAGAC 
AGGATCTCCA AGGCCGGGCC GCTTCCTCTA CCTGCTAAGA TGGTGGAGGA GATGCCGAAA 
GAGCAGAGCG CAGCCAGAGA AACAGAGAAA CAGCTCCCCC CTCTGCCAGA GCCCAAACCT 
GTGTACGCTC AGCCGGGCCA GCCGGACGTG GACCTGCCTG TTAGTCCGGC AGATGCTCCT 
GTGCCCAGCG TCACACATGA TGACAGCATC TTACGGCCGA GCATGAAGCT GGTGAAGTTC 
AAGAAGGGCG AGAGCGTGGG CTTGAGGCTC GCTGGGGGGA ACGACGTTGG GATATTTGTG 
GCGGGAGTTC TGGAGGACAG TCCTGCTGCT AAAGAGGGCC TGGAAGAGGG AGACCAGATT 
CTCAGGGTAA ATAATGTGGA TTTTGCAAAT ATCATCCGAG AGGAGGCAGT GCTGTTCCTG 
CTGGATCTGC CCAAAGGAGA GGAGGTCACC ATCCTGGCCC AGAAGAAGAA AGACGTGTAC 
CGCCGCATTG TAGAGTCTGA CGTGGGAGAC TCGTTCTACA TCAGGACTCA TTTTGAATAT 
GAGAAGGAGT CGCCCTATGG CCTGAGCTTC AATAAGGGGG AGGTCTTCAG AGTCGTCGAC 
ACCCTTTACA ATGGCAAACT GGGCTCCTGG CTCGCCATCC GCATCGGCAA AAACCACCAG 
GAGGTGGAGC GGGGCATCAT CCCCAACAAG AACAGGGCTG AGCAGTTATC AAGTGTCCAG 
TACACTCTCC CAAAGACTCC AGGAGGGGAT CGCGCCGACT TCTGGAGATT CAGAGGCCTC 
CGCAGCTCCA AACGAAACCT GAGGAAGAGC AGAGAAGACC TGTCTGCTCA GCCGGTCCAA 
ACCAAATTCC CTGCTTATGA AAGAGTGGTG CTTAGAGAAG CTGGTTTCTT GAGGCCAGTG 
GTCATTTTCG GACCGATCGC GGATGTGGCT AGAGAGAAGC TGTCCAGAGA GGAGACTGAC 
CTCTTCGAAC TTGCAAAGAG TGAACCCAGA GATGCGGGCA CAGACCAGCG CAGCTCCGGA 
ATCATCCGTC TCCATACCAT TAAACAGATC ATTGACCGAG ACAAGCACGC GGTGCTGGAC 
ATCACCCCTA ATGCGGTGGA CCGTCTGAAC TACGCTCAGT GGTACCCCAT CGTGGTGTTT 
CTGAATCCAG ACAGCAAACA GGGCGTCAAG AACATGAGGA CCAGGCTCTG TCCCGAGTCC 
AGGAAGAGCG CCCGAAAGCT CTACGAGCGT GCTCTCAAAC TGAGGAAGAA CAACCATCAC 
CTTTTCACCA CCACTATTAA CTTGAACAAC ATGAATGACG GGTGGTACGG TGCTTTGAAA 
GAGAGCATCC AGCAGCAGCA GAACCAGTTG GTCTGGGTCT CAGAGGGCAA GGCCGACGGC 
GCTCCTGACG ATGACCTTGA CATCCATGAC GACCGCCTCT CCTACCTCTC GGCTCCGGGC 
AGCGAGTACA GCATGTACAG CACCGACAGC CGCCACACGT CCGACTATGA GGACACCGAC 
ACGGAGGGTG GTGCCTACAC CGACCAGGAG CTGGACGAGA CCATGAACGA TGAGGTGGGC 
CTGCCTAGCG AGCCTGCCAT CACACGCTCA TCTGAGCCTG TGCGAGAGGA CCCGCCAGTC 
ATTCAGGACG CAGCTGGATA CCCCAGCTAC CAGCATGCGG TACCGCAACC TGAAGCGGTG 
AGCCGCATTG ACCCGGCCGG GTTTAAGATG GCAGCGCCGC AGCAGCAAGC TGAGGCTGCT 
CTGGCCTCGC CGCCCCCTCC CTCTGCAGCG GTCCAGCCCA CCGCAACACT AGCGGGTACG 
AACCCCGAGG AGCCGCCTGG CACCCCTCAG GCCGACTCCC TTAACAGCCC CGTCCCTGTT 
CCCGACCCCC AGCCCGAACC TGAGCTCGCT CAGCCCCCAA CACACGACCC CCACCAGTCG 
CTTCCTCCTG GCCCAGATCC AAAGATGTAT AAAAAGGATC TGTACAGCAT GGACGAGCCG 
GTGCGAGTGA CCCACGGCGT GAAGCCTCCC CCTCCCCAGC AGCAGCAGCA GCCTTTAGGC 
CCCCCAACCT CGCTCTCCTA CAGCCACCAG CCTGTCTACC AGGACCAACA GCCATACCGC 
CAGTACGACC ACCCGCCTTA TGGCTATGAC GGTGGCGGCT ACGCACAACC AAAGCCTCAC 
AACTACGACC CGCACCTGCA CTACGACAAC CGTGTGCCTC ACTATAACGA GCAGTGGCCC 
ACGTACGACC AGCAGCAGCA GCAGCAGACT TCCCCCCAGC CGCCACCTGC GTCCGGCTTC 
CCTCAGGGCC ACCAGCCTCC GCCTCCACCA CCGATGGGAT ATGAGCCACG CTCCCCATAC 
GAGGACGGAC CTAACAGGGA TTTCAGCCCT CCTCAGTCCC AGTATGACGG TGTGTCCCCA 
ATGGGCTACG ATCACCGGCC GCGGCACCCC AAACCTGCAC CGGTCCGATA TGAAGAGCCT 
CCACCTCCAC CTCCTCCGGT GTCCTACGAT GCACGGTCAC CTTTCGAACC CGAGCCGCAT 
GCTTTCCCAG GCAACACACA TCGCTCGCCA GACCCACCGA AACAGTATTA TGGAGACGCC 
GCTATGAGGC CCTTGTACAA CCCTGGACCT CCGAACAGAG TGTACAAACA TGAGCCGGTG 
AACTCTGAGC CTCCTGTACC GCCTCCCAAA CCTGAATCTG TCCTGTCTCC AGGAGAACCG 
CCCCATTCTG CAGCTTCCAA ACCATTGCCG CCTCCTCCCA GAGATGATGA CGATGATGAT 
GACGATGATG ATGACCCTGC CATGAAGCCC CAGTCTGTGC TCAACAGGGT CAAAATGTTC 
GAAAACAAAC GCTCTGTGTC GGTAGACCGA GCCAAGGACG CACCTGACGT CACTGGAATT 
AGGCCCACAG ATCTCCCCAA ACCTGCGAGC ACCCCTGGAC CTGTGCTCAA AGCCAACTCT 
CTCAGCAACC TGGAGCAGGA CAAACCCTCC TACAGAGCTC CAGAGCCGCA GAAACCTCAG 
ACGCGTGTTG GGGACGATGT GGTTCGCTCC AACCACTACG ACCCTGATGA TGATGAAGAG 
TACTACAGAA AACAGCTCTC ATTTTTCGAC CGCCGCAGTT TCGACAGTAA GGTCATGAAT 
CCACCCAGCG CTGGAGTAAA CCGCTTCCAT GAGCCGCCCA AACCACCCCA GACACAGATC 
GCTTATCCTT TCGCTAGGAC GGAGTCTGTG GAGAAGGTGA GTCCTGTGGA CAAGAGATAC 
GAGCCTCTGC CGCCAGTCAA CCCTTCACCT GCTCCATACA GCCAGAACAC ACCTGTCGCT 
CCGCCCACAT CCCTGCCCAA ACTCACCAAC ATCGAGGTGA ACTCTCTGCC AGATCCTCAC 
AGCTCTCCCA AAGCCAAACC GGACCTGTCT GCTCTGAGAG CTCCGACCCG GGACGAGCCC 
ATCCAGAGCA GCTACTTGCC CCCCAAGTCC ACCATCAATG GCATGGATGC TCCTCCCAAA 
ACACTCGGCG TGCCGACCAG CTACAACCGC TACGTCCCCA AGCCTTACAC CAGCTCGGCG 
CGACCCTTCG AGCGCAAGTT CGAGAGTCCG AAATTCAACC ACAACCTGCT TCCCAACGAC 
ACGCAGTCCA AACCCAGTGT CAACAACAAC CTGAAGCCTC AGATCTCCCC ACAACCCCAG 
GACACGGACA GCGGCGTCGA CACCTTCACT CGCACCATGG ACAACAGGCC AAAATATCAG 
CACAACAACA TCAACGCCAT TCCCAAAGCC GTCCCAGTCA GCCCCAGTGC ACTGGATGAT 
GATGAAGAAG ATGAGGGACA CACAGTTGTG GCCACGGCGC GGGGCATCTT TAACTGTAAT 
GGCGGGGTGT TGAGCTCTAT AGAGACGGGC GTCAGCATCA TTATCCCACA GGGAGCCATT 
CCTGATAGTG TGGAGCAGGA GATCTACTTC AAAGTGTGCC GGGACAACAG CATCCTGCCT 
CCTCTGGATA AGGAGAAAGG TGAAACTCTG CTGAGTCCGC TGGTGATGTG TGGTCCTCAC 
GGCCTGAAGT TCCTGAAGCC TGTGGAGCTG CGCTTGCCAC ACTGTGCGTC TATGACCCCT 
GATGGTTGGT CTTTTGCTCT AAAATCCTCC GACTCCTCGT CGGGTGACCC CAAATGCTGG 
CAAAACAAGT GTCTACCAGG AGACCCCAAT TACCTGGTGG GCGCAAACTG TGTGTCTGTG 
CTGATCGACC ACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326406.1
CDS	110..6187
Translation	MLLSVFGGPVEEVLAGLLKLKMFGSGSKKWFIKKNKRQRTKVQRKPASGRRNKRVSRVLKRHKEVIVKQLDVSKVLPTLVYERVFTLGEYKEILGQDSSGKRAELFLDLLSQKEPAAFSAFCGVLEEVCPHLLTCFLLDGTDGSAGENKKQGQTKPAHSQQNALSKGQDTVCFPPMYTDPLFDTRVFPKHDKDKYGPAVYYPKFNAYIPNPDKVSVLDESEGSGHGKPSLRRIKGRIHRSKSLDSIDLLDSNSAAMDETVIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGLLQENDRVVMVNAVSMDNVDHAYAVQQLRKSGKNAKITIRRKRKVQIPSGRPGERETMSEHDEDDDSYEDEIYESRSARSGPSAYSGGGGGGGGGGASGRRSGRGDRLSGRRERDRERSGSRERSLSPRSDRHSVSSNLPPRPAKVTLVKSRKNEACMASGSSQKVTSAEYGLRLASHIFVKDISPESLAARDGNIQEGDVVLKKLIERSKGKLKMVVQRDERATLLNIPDLDDSIPSANASDRDDIHSVASDHSNRSHDRKRSSRSRSPDRRSEPSDHSRHSPPQISNGRATGPTAQRTRPVHRSRDEDRISKAGPLPLPAKMVEEMPKEQSAARETEKQLPPLPEPKPVYAQPGQPDVDLPVSPADAPVPSVTHDDSILRPSMKLVKFKKGESVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFANIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHQEVERGIIPNKNRAEQLSSVQYTLPKTPGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVVIFGPIADVAREKLSREETDLFELAKSEPRDAGTDQRSSGIIRLHTIKQIIDRDKHAVLDITPNAVDRLNYAQWYPIVVFLNPDSKQGVKNMRTRLCPESRKSARKLYERALKLRKNNHHLFTTTINLNNMNDGWYGALKESIQQQQNQLVWVSEGKADGAPDDDLDIHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETMNDEVGLPSEPAITRSSEPVREDPPVIQDAAGYPSYQHAVPQPEAVSRIDPAGFKMAAPQQQAEAALASPPPPSAAVQPTATLAGTNPEEPPGTPQADSLNSPVPVPDPQPEPELAQPPTHDPHQSLPPGPDPKMYKKDLYSMDEPVRVTHGVKPPPPQQQQQPLGPPTSLSYSHQPVYQDQQPYRQYDHPPYGYDGGGYAQPKPHNYDPHLHYDNRVPHYNEQWPTYDQQQQQQTSPQPPPASGFPQGHQPPPPPPMGYEPRSPYEDGPNRDFSPPQSQYDGVSPMGYDHRPRHPKPAPVRYEEPPPPPPPVSYDARSPFEPEPHAFPGNTHRSPDPPKQYYGDAAMRPLYNPGPPNRVYKHEPVNSEPPVPPPKPESVLSPGEPPHSAASKPLPPPPRDDDDDDDDDDDPAMKPQSVLNRVKMFENKRSVSVDRAKDAPDVTGIRPTDLPKPASTPGPVLKANSLSNLEQDKPSYRAPEPQKPQTRVGDDVVRSNHYDPDDDEEYYRKQLSFFDRRSFDSKVMNPPSAGVNRFHEPPKPPQTQIAYPFARTESVEKVSPVDKRYEPLPPVNPSPAPYSQNTPVAPPTSLPKLTNIEVNSLPDPHSSPKAKPDLSALRAPTRDEPIQSSYLPPKSTINGMDAPPKTLGVPTSYNRYVPKPYTSSARPFERKFESPKFNHNLLPNDTQSKPSVNNNLKPQISPQPQDTDSGVDTFTRTMDNRPKYQHNNINAIPKAVPVSPSALDDDEEDEGHTVVATARGIFNCNGGVLSSIETGVSIIIPQGAIPDSVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKCWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_021470731.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tight junction protein 1b (tjp1b), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470731.1

ORF Insert Sequence:

ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
TCCCGCAAGA ATGAAGCATG TATGGCGTCA GGAAGTTCTC AGAAGGTGAC ATCAGCAGAA 
TACGGGCTTC GCCTGGCCAG CCACATCTTC GTGAAGGACA TTTCCCCCGA GAGCCTGGCA 
GCCCGAGACG GAAACATCCA AGAGGGAGAC GTGGTGCTTA AGAAGCTGAT CGAGAGGTCA 
AAGGGAAAGC TGAAGATGGT GGTGCAGAGA GACGAGCGGG CGACGCTGCT GAACATCCCC 
GATCTGGACG ACAGCATACC TTCTGCTAAT GCCTCGGACA GAGATGACAT CCACTCCGTG 
GCCTCTGATC ATTCCAACCG ATCCCACGAC CGGAAGAGGA GCAGCCGCTC TCGTTCTCCA 
GACCGCCGCT CCGAACCCTC AGACCACTCC AGACACTCTC CACCACAGAT CAGCAACGGC 
AGGGCCACAG GGCCGACGGC TCAGAGAACG CGGCCAGTTC ACAGGAGCCG TGATGAAGAC 
AGGATCTCCA AGGCCGGGCC GCTTCCTCTA CCTGCTAAGA TGGTGGAGGA GATGCCGAAA 
GAGCAGAGCG CAGCCAGAGA AACAGAGAAA CAGCTCCCCC CTCTGCCAGA GCCCAAACCT 
GTGTACGCTC AGCCGGGCCA GCCGGACGTG GACCTGCCTG TTAGTCCGGC AGATGCTCCT 
GTGCCCAGCG TCACACATGA TGACAGCATC TTACGGCCGA GCATGAAGCT GGTGAAGTTC 
AAGAAGGGCG AGAGCGTGGG CTTGAGGCTC GCTGGGGGGA ACGACGTTGG GATATTTGTG 
GCGGGAGTTC TGGAGGACAG TCCTGCTGCT AAAGAGGGCC TGGAAGAGGG AGACCAGATT 
CTCAGGGTAA ATAATGTGGA TTTTGCAAAT ATCATCCGAG AGGAGGCAGT GCTGTTCCTG 
CTGGATCTGC CCAAAGGAGA GGAGGTCACC ATCCTGGCCC AGAAGAAGAA AGACGTGTAC 
CGCCGCATTG TAGAGTCTGA CGTGGGAGAC TCGTTCTACA TCAGGACTCA TTTTGAATAT 
GAGAAGGAGT CGCCCTATGG CCTGAGCTTC AATAAGGGGG AGGTCTTCAG AGTCGTCGAC 
ACCCTTTACA ATGGCAAACT GGGCTCCTGG CTCGCCATCC GCATCGGCAA AAACCACCAG 
GAGGTGGAGC GGGGCATCAT CCCCAACAAG AACAGGGCTG AGCAGTTATC AAGTGTCCAG 
TACACTCTCC CAAAGACTCC AGGAGGGGAT CGCGCCGACT TCTGGAGATT CAGAGGCCTC 
CGCAGCTCCA AACGAAACCT GAGGAAGAGC AGAGAAGACC TGTCTGCTCA GCCGGTCCAA 
ACCAAATTCC CTGCTTATGA AAGAGTGGTG CTTAGAGAAG CTGGTTTCTT GAGGCCAGTG 
GTCATTTTCG GACCGATCGC GGATGTGGCT AGAGAGAAGC TGTCCAGAGA GGAGACTGAC 
CTCTTCGAAC TTGCAAAGAG TGAACCCAGA GATGCGGGCA CAGACCAGCG CAGCTCCGGA 
ATCATCCGTC TCCATACCAT TAAACAGATC ATTGACCGAG ACAAGCACGC GGTGCTGGAC 
ATCACCCCTA ATGCGGTGGA CCGTCTGAAC TACGCTCAGT GGTACCCCAT CGTGGTGTTT 
CTGAATCCAG ACAGCAAACA GGGCGTCAAG AACATGAGGA CCAGGCTCTG TCCCGAGTCC 
AGGAAGAGCG CCCGAAAGCT CTACGAGCGT GCTCTCAAAC TGAGGAAGAA CAACCATCAC 
CTTTTCACCA CCACTATTAA CTTGAACAAC ATGAATGACG GGTGGTACGG TGCTTTGAAA 
GAGAGCATCC AGCAGCAGCA GAACCAGTTG GTCTGGGTCT CAGAGGGCAA GGCCGACGGC 
GCTCCTGACG ATGACCTTGA CATCCATGAC GACCGCCTCT CCTACCTCTC GGCTCCGGGC 
AGCGAGTACA GCATGTACAG CACCGACAGC CGCCACACGT CCGACTATGA GGACACCGAC 
ACGGAGGGTG GTGCCTACAC CGACCAGGAG CTGGACGAGA CCATGAACGA TGAGGTGGGC 
CTGCCTAGCG AGCCTGCCAT CACACGCTCA TCTGAGCCTG TGCGAGAGGA CCCGCCAGTC 
ATTCAGGACG CAGCTGGATA CCCCAGCTAC CAGCATGCGG TACCGCAACC TGAAGCGGTG 
AGCCGCATTG ACCCGGCCGG GTTTAAGATG GCAGCGCCGC AGCAGCAAGC TGAGGCTGCT 
CTGGCCTCGC CGCCCCCTCC CTCTGCAGCG GTCCAGCCCA CCGCAACACT AGCGGGTACG 
AACCCCGAGG AGCCGCCTGG CACCCCTCAG GCCGACTCCC TTAACAGCCC CGTCCCTGTT 
CCCGACCCCC AGCCCGAACC TGAGCTCGCT CAGCCCCCAA CACACGACCC CCACCAGTCG 
CTTCCTCCTG GCCCAGATCC AAAGATGTAT AAAAAGGATC TGTACAGCAT GGACGAGCCG 
GTGCGAGTGA CCCACGGCGT GAAGCCTCCC CCTCCCCAGC AGCAGCAGCA GCCTTTAGGC 
CCCCCAACCT CGCTCTCCTA CAGCCACCAG CCTGTCTACC AGGACCAACA GCCATACCGC 
CAGTACGACC ACCCGCCTTA TGGCTATGAC GGTGGCGGCT ACGCACAACC AAAGCCTCAC 
AACTACGACC CGCACCTGCA CTACGACAAC CGTGTGCCTC ACTATAACGA GCAGTGGCCC 
ACGTACGACC AGCAGCAGCA GCAGCAGACT TCCCCCCAGC CGCCACCTGC GTCCGGCTTC 
CCTCAGGGCC ACCAGCCTCC GCCTCCACCA CCGATGGGAT ATGAGCCACG CTCCCCATAC 
GAGGACGGAC CTAACAGGGA TTTCAGCCCT CCTCAGTCCC AGTATGACGG TGTGTCCCCA 
ATGGGCTACG ATCACCGGCC GCGGCACCCC AAACCTGCAC CGGTCCGATA TGAAGAGCCT 
CCACCTCCAC CTCCTCCGGT GTCCTACGAT GCACGGTCAC CTTTCGAACC CGAGCCGCAT 
GCTTTCCCAG GCAACACACA TCGCTCGCCA GACCCACCGA AACAGTATTA TGGAGACGCC 
GCTATGAGGC CCTTGTACAA CCCTGGACCT CCGAACAGAG TGTACAAACA TGAGCCGGTG 
AACTCTGAGC CTCCTGTACC GCCTCCCAAA CCTGAATCTG TCCTGTCTCC AGGAGAACCG 
CCCCATTCTG CAGCTTCCAA ACCATTGCCG CCTCCTCCCA GAGATGATGA CGATGATGAT 
GACGATGATG ATGACCCTGC CATGAAGCCC CAGTCTGTGC TCAACAGGGT CAAAATGTTC 
GAAAACAAAC GCTCTGTGTC GGTAGACCGA GCCAAGGACG CACCTGACGT CACTGGAATT 
AGGCCCACAG ATCTCCCCAA ACCTGCGAGC ACCCCTGGAC CTGTGCTCAA AGCCAACTCT 
CTCAGCAACC TGGAGCAGGA CAAACCCTCC TACAGAGCTC CAGAGCCGCA GAAACCTCAG 
ACGCGTGTTG GGGACGATGT GGTTCGCTCC AACCACTACG ACCCTGATGA TGATGAAGAG 
TACTACAGAA AACAGCTCTC ATTTTTCGAC CGCCGCAGTT TCGACAGTAA GGTCATGAAT 
CCACCCAGCG CTGGAGTAAA CCGCTTCCAT GAGCCGCCCA AACCACCCCA GACACAGATC 
GCTTATCCTT TCGCTAGGAC GGAGTCTGTG GAGAAGGTGA GTCCTGTGGA CAAGAGATAC 
GAGCCTCTGC CGCCAGTCAA CCCTTCACCT GCTCCATACA GCCAGAACAC ACCTGTCGCT 
CCGCCCACAT CCCTGCCCAA ACTCACCAAC ATCGAGGTGA ACTCTCTGCC AGATCCTCAC 
AGCTCTCCCA AAGCCAAACC GGACCTGTCT GCTCTGAGAG CTCCGACCCG GGACGAGCCC 
ATCCAGAGCA GCTACTTGCC CCCCAAGTCC ACCATCAATG GCATGGATGC TCCTCCCAAA 
ACACTCGGCG TGCCGACCAG CTACAACCGC TACGTCCCCA AGCCTTACAC CAGCTCGGCG 
CGACCCTTCG AGCGCAAGTT CGAGAGTCCG AAATTCAACC ACAACCTGCT TCCCAACGAC 
ACGCAGTCCA AACCCAGTGT CAACAACAAC CTGAAGCCTC AGATCTCCCC ACAACCCCAG 
GACACGGACA GCGGCGTCGA CACCTTCACT CGCACCATGG ACAACAGGCC AAAATATCAG 
CACAACAACA TCAACGCCAT TCCCAAAGCC GTCCCAGTCA GCCCCAGTGC ACTGGATGAT 
GATGAAGAAG ATGAGGGACA CACAGTTGTG GCCACGGCGC GGGGCATCTT TAACTGTAAT 
GGCGGGGTGT TGAGCTCTAT AGAGACGGGC GTCAGCATCA TTATCCCACA GGGAGCCATT 
CCTGATAGTG TGGAGCAGGA GATCTACTTC AAAGTGTGCC GGGACAACAG CATCCTGCCT 
CCTCTGGATA AGGAGAAAGG TGAAACTCTG CTGAGTCCGC TGGTGATGTG TGGTCCTCAC 
GGCCTGAAGT TCCTGAAGCC TGTGGAGCTG CGCTTGCCAC ACTGTGCGTC TATGACCCCT 
GATGGTTGGT CTTTTGCTCT AAAATCCTCC GACTCCTCGT CGGGTGACCC CAAATGCTGG 
CAAAACAAGT GTCTACCAGG AGACCCCAAT TACCTGGTGG GCGCAAACTG TGTGTCTGTG 
CTGATCGACC ACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp1b ( XM_021470723.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326398.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X1, mRNA.

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CloneID	ODa44616
Clone ID Related Accession (Same CDS sequence)	XM_021470723.1
Accession Version	XM_021470723.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6132bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tight junction protein ZO-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
TCCCGCAAGA ATGAAGCATG TATGGCGTCA GGAAGTTCTC AGAAGGTGAC ATCAGCAGAA 
TACGGGCTTC GCCTGGCCAG CCACATCTTC GTGAAGGACA TTTCCCCCGA GAGCCTGGCA 
GCCCGAGACG GAAACATCCA AGAGGGAGAC GTGGTGCTTA AGATTAATGG CACGGTAACA 
GAGAATTTGT CCCTGATCGA TGCTAAGAAG CTGATCGAGA GGTCAAAGGG AAAGCTGAAG 
ATGGTGGTGC AGAGAGACGA GCGGGCGACG CTGCTGAACA TCCCCGATCT GGACGACAGC 
ATACCTTCTG CTAATGCCTC GGACAGAGAT GATATTTCAG ACATCCACTC CGTGGCCTCT 
GATCATTCCA ACCGATCCCA CGACCGGAAG AGGAGCAGCC GCTCTCGTTC TCCAGACCGC 
CGCTCCGAAC CCTCAGACCA CTCCAGACAC TCTCCACCAC AGATCAGCAA CGGCAGGGCC 
ACAGGGCCGA CGGCTCAGAG AACGCGGCCA GTTCACAGGA GCCGTGATGA AGACAGGATC 
TCCAAGGCCG GGCCGCTTCC TCTACCTGCT AAGATGGTGG AGGAGATGCC GAAAGAGCAG 
AGCGCAGCCA GAGAAACAGA GAAACAGCTC CCCCCTCTGC CAGAGCCCAA ACCTGTGTAC 
GCTCAGCCGG GCCAGCCGGA CGTGGACCTG CCTGTTAGTC CGGCAGATGC TCCTGTGCCC 
AGCGTCACAC ATGATGACAG CATCTTACGG CCGAGCATGA AGCTGGTGAA GTTCAAGAAG 
GGCGAGAGCG TGGGCTTGAG GCTCGCTGGG GGGAACGACG TTGGGATATT TGTGGCGGGA 
GTTCTGGAGG ACAGTCCTGC TGCTAAAGAG GGCCTGGAAG AGGGAGACCA GATTCTCAGG 
GTAAATAATG TGGATTTTGC AAATATCATC CGAGAGGAGG CAGTGCTGTT CCTGCTGGAT 
CTGCCCAAAG GAGAGGAGGT CACCATCCTG GCCCAGAAGA AGAAAGACGT GTACCGCCGC 
ATTGTAGAGT CTGACGTGGG AGACTCGTTC TACATCAGGA CTCATTTTGA ATATGAGAAG 
GAGTCGCCCT ATGGCCTGAG CTTCAATAAG GGGGAGGTCT TCAGAGTCGT CGACACCCTT 
TACAATGGCA AACTGGGCTC CTGGCTCGCC ATCCGCATCG GCAAAAACCA CCAGGAGGTG 
GAGCGGGGCA TCATCCCCAA CAAGAACAGG GCTGAGCAGT TATCAAGTGT CCAGTACACT 
CTCCCAAAGA CTCCAGGAGG GGATCGCGCC GACTTCTGGA GATTCAGAGG CCTCCGCAGC 
TCCAAACGAA ACCTGAGGAA GAGCAGAGAA GACCTGTCTG CTCAGCCGGT CCAAACCAAA 
TTCCCTGCTT ATGAAAGAGT GGTGCTTAGA GAAGCTGGTT TCTTGAGGCC AGTGGTCATT 
TTCGGACCGA TCGCGGATGT GGCTAGAGAG AAGCTGTCCA GAGAGGAGAC TGACCTCTTC 
GAACTTGCAA AGAGTGAACC CAGAGATGCG GGCACAGACC AGCGCAGCTC CGGAATCATC 
CGTCTCCATA CCATTAAACA GATCATTGAC CGAGACAAGC ACGCGGTGCT GGACATCACC 
CCTAATGCGG TGGACCGTCT GAACTACGCT CAGTGGTACC CCATCGTGGT GTTTCTGAAT 
CCAGACAGCA AACAGGGCGT CAAGAACATG AGGACCAGGC TCTGTCCCGA GTCCAGGAAG 
AGCGCCCGAA AGCTCTACGA GCGTGCTCTC AAACTGAGGA AGAACAACCA TCACCTTTTC 
ACCACCACTA TTAACTTGAA CAACATGAAT GACGGGTGGT ACGGTGCTTT GAAAGAGAGC 
ATCCAGCAGC AGCAGAACCA GTTGGTCTGG GTCTCAGAGG GCAAGGCCGA CGGCGCTCCT 
GACGATGACC TTGACATCCA TGACGACCGC CTCTCCTACC TCTCGGCTCC GGGCAGCGAG 
TACAGCATGT ACAGCACCGA CAGCCGCCAC ACGTCCGACT ATGAGGACAC CGACACGGAG 
GGTGGTGCCT ACACCGACCA GGAGCTGGAC GAGACCATGA ACGATGAGGT GGGCCTGCCT 
AGCGAGCCTG CCATCACACG CTCATCTGAG CCTGTGCGAG AGGACCCGCC AGTCATTCAG 
GACGCAGCTG GATACCCCAG CTACCAGCAT GCGGTACCGC AACCTGAAGC GGTGAGCCGC 
ATTGACCCGG CCGGGTTTAA GATGGCAGCG CCGCAGCAGC AAGCTGAGGC TGCTCTGGCC 
TCGCCGCCCC CTCCCTCTGC AGCGGTCCAG CCCACCGCAA CACTAGCGGG TACGAACCCC 
GAGGAGCCGC CTGGCACCCC TCAGGCCGAC TCCCTTAACA GCCCCGTCCC TGTTCCCGAC 
CCCCAGCCCG AACCTGAGCT CGCTCAGCCC CCAACACACG ACCCCCACCA GTCGCTTCCT 
CCTGGCCCAG ATCCAAAGAT GTATAAAAAG GATCTGTACA GCATGGACGA GCCGGTGCGA 
GTGACCCACG GCGTGAAGCC TCCCCCTCCC CAGCAGCAGC AGCAGCCTTT AGGCCCCCCA 
ACCTCGCTCT CCTACAGCCA CCAGCCTGTC TACCAGGACC AACAGCCATA CCGCCAGTAC 
GACCACCCGC CTTATGGCTA TGACGGTGGC GGCTACGCAC AACCAAAGCC TCACAACTAC 
GACCCGCACC TGCACTACGA CAACCGTGTG CCTCACTATA ACGAGCAGTG GCCCACGTAC 
GACCAGCAGC AGCAGCAGCA GACTTCCCCC CAGCCGCCAC CTGCGTCCGG CTTCCCTCAG 
GGCCACCAGC CTCCGCCTCC ACCACCGATG GGATATGAGC CACGCTCCCC ATACGAGGAC 
GGACCTAACA GGGATTTCAG CCCTCCTCAG TCCCAGTATG ACGGTGTGTC CCCAATGGGC 
TACGATCACC GGCCGCGGCA CCCCAAACCT GCACCGGTCC GATATGAAGA GCCTCCACCT 
CCACCTCCTC CGGTGTCCTA CGATGCACGG TCACCTTTCG AACCCGAGCC GCATGCTTTC 
CCAGGCAACA CACATCGCTC GCCAGACCCA CCGAAACAGT ATTATGGAGA CGCCGCTATG 
AGGCCCTTGT ACAACCCTGG ACCTCCGAAC AGAGTGTACA AACATGAGCC GGTGAACTCT 
GAGCCTCCTG TACCGCCTCC CAAACCTGAA TCTGTCCTGT CTCCAGGAGA ACCGCCCCAT 
TCTGCAGCTT CCAAACCATT GCCGCCTCCT CCCAGAGATG ATGACGATGA TGATGACGAT 
GATGATGACC CTGCCATGAA GCCCCAGTCT GTGCTCAACA GGGTCAAAAT GTTCGAAAAC 
AAACGCTCTG TGTCGGTAGA CCGAGCCAAG GACGCACCTG ACGTCACTGG AATTAGGCCC 
ACAGATCTCC CCAAACCTGC GAGCACCCCT GGACCTGTGC TCAAAGCCAA CTCTCTCAGC 
AACCTGGAGC AGGACAAACC CTCCTACAGA GCTCCAGAGC CGCAGAAACC TCAGACGCGT 
GTTGGGGACG ATGTGGTTCG CTCCAACCAC TACGACCCTG ATGATGATGA AGAGTACTAC 
AGAAAACAGC TCTCATTTTT CGACCGCCGC AGTTTCGACA GTAAGGTCAT GAATCCACCC 
AGCGCTGGAG TAAACCGCTT CCATGAGCCG CCCAAACCAC CCCAGACACA GATCGCTTAT 
CCTTTCGCTA GGACGGAGTC TGTGGAGAAG GTGAGTCCTG TGGACAAGAG ATACGAGCCT 
CTGCCGCCAG TCAACCCTTC ACCTGCTCCA TACAGCCAGA ACACACCTGT CGCTCCGCCC 
ACATCCCTGC CCAAACTCAC CAACATCGAG GTGAACTCTC TGCCAGATCC TCACAGCTCT 
CCCAAAGCCA AACCGGACCT GTCTGCTCTG AGAGCTCCGA CCCGGGACGA GCCCATCCAG 
AGCAGCTACT TGCCCCCCAA GTCCACCATC AATGGCATGG ATGCTCCTCC CAAAACACTC 
GGCGTGCCGA CCAGCTACAA CCGCTACGTC CCCAAGCCTT ACACCAGCTC GGCGCGACCC 
TTCGAGCGCA AGTTCGAGAG TCCGAAATTC AACCACAACC TGCTTCCCAA CGACACGCAG 
TCCAAACCCA GTGTCAACAA CAACCTGAAG CCTCAGATCT CCCCACAACC CCAGGACACG 
GACAGCGGCG TCGACACCTT CACTCGCACC ATGGACAACA GGCCAAAATA TCAGCACAAC 
AACATCAACG CCATTCCCAA AGCCGTCCCA GTCAGCCCCA GTGCACTGGA TGATGATGAA 
GAAGATGAGG GACACACAGT TGTGGCCACG GCGCGGGGCA TCTTTAACTG TAATGGCGGG 
GTGTTGAGCT CTATAGAGAC GGGCGTCAGC ATCATTATCC CACAGGGAGC CATTCCTGAT 
AGTGTGGAGC AGGAGATCTA CTTCAAAGTG TGCCGGGACA ACAGCATCCT GCCTCCTCTG 
GATAAGGAGA AAGGTGAAAC TCTGCTGAGT CCGCTGGTGA TGTGTGGTCC TCACGGCCTG 
AAGTTCCTGA AGCCTGTGGA GCTGCGCTTG CCACACTGTG CGTCTATGAC CCCTGATGGT 
TGGTCTTTTG CTCTAAAATC CTCCGACTCC TCGTCGGGTG ACCCCAAATG CTGGCAAAAC 
AAGTGTCTAC CAGGAGACCC CAATTACCTG GTGGGCGCAA ACTGTGTGTC TGTGCTGATC 
GACCACTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326398.1
CDS	110..6241
Translation	MLLSVFGGPVEEVLAGLLKLKMFGSGSKKWFIKKNKRQRTKVQRKPASGRRNKRVSRVLKRHKEVIVKQLDVSKVLPTLVYERVFTLGEYKEILGQDSSGKRAELFLDLLSQKEPAAFSAFCGVLEEVCPHLLTCFLLDGTDGSAGENKKQGQTKPAHSQQNALSKGQDTVCFPPMYTDPLFDTRVFPKHDKDKYGPAVYYPKFNAYIPNPDKVSVLDESEGSGHGKPSLRRIKGRIHRSKSLDSIDLLDSNSAAMDETVIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGLLQENDRVVMVNAVSMDNVDHAYAVQQLRKSGKNAKITIRRKRKVQIPSGRPGERETMSEHDEDDDSYEDEIYESRSARSGPSAYSGGGGGGGGGGASGRRSGRGDRLSGRRERDRERSGSRERSLSPRSDRHSVSSNLPPRPAKVTLVKSRKNEACMASGSSQKVTSAEYGLRLASHIFVKDISPESLAARDGNIQEGDVVLKINGTVTENLSLIDAKKLIERSKGKLKMVVQRDERATLLNIPDLDDSIPSANASDRDDISDIHSVASDHSNRSHDRKRSSRSRSPDRRSEPSDHSRHSPPQISNGRATGPTAQRTRPVHRSRDEDRISKAGPLPLPAKMVEEMPKEQSAARETEKQLPPLPEPKPVYAQPGQPDVDLPVSPADAPVPSVTHDDSILRPSMKLVKFKKGESVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFANIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHQEVERGIIPNKNRAEQLSSVQYTLPKTPGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVVIFGPIADVAREKLSREETDLFELAKSEPRDAGTDQRSSGIIRLHTIKQIIDRDKHAVLDITPNAVDRLNYAQWYPIVVFLNPDSKQGVKNMRTRLCPESRKSARKLYERALKLRKNNHHLFTTTINLNNMNDGWYGALKESIQQQQNQLVWVSEGKADGAPDDDLDIHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETMNDEVGLPSEPAITRSSEPVREDPPVIQDAAGYPSYQHAVPQPEAVSRIDPAGFKMAAPQQQAEAALASPPPPSAAVQPTATLAGTNPEEPPGTPQADSLNSPVPVPDPQPEPELAQPPTHDPHQSLPPGPDPKMYKKDLYSMDEPVRVTHGVKPPPPQQQQQPLGPPTSLSYSHQPVYQDQQPYRQYDHPPYGYDGGGYAQPKPHNYDPHLHYDNRVPHYNEQWPTYDQQQQQQTSPQPPPASGFPQGHQPPPPPPMGYEPRSPYEDGPNRDFSPPQSQYDGVSPMGYDHRPRHPKPAPVRYEEPPPPPPPVSYDARSPFEPEPHAFPGNTHRSPDPPKQYYGDAAMRPLYNPGPPNRVYKHEPVNSEPPVPPPKPESVLSPGEPPHSAASKPLPPPPRDDDDDDDDDDDPAMKPQSVLNRVKMFENKRSVSVDRAKDAPDVTGIRPTDLPKPASTPGPVLKANSLSNLEQDKPSYRAPEPQKPQTRVGDDVVRSNHYDPDDDEEYYRKQLSFFDRRSFDSKVMNPPSAGVNRFHEPPKPPQTQIAYPFARTESVEKVSPVDKRYEPLPPVNPSPAPYSQNTPVAPPTSLPKLTNIEVNSLPDPHSSPKAKPDLSALRAPTRDEPIQSSYLPPKSTINGMDAPPKTLGVPTSYNRYVPKPYTSSARPFERKFESPKFNHNLLPNDTQSKPSVNNNLKPQISPQPQDTDSGVDTFTRTMDNRPKYQHNNINAIPKAVPVSPSALDDDEEDEGHTVVATARGIFNCNGGVLSSIETGVSIIIPQGAIPDSVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKCWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_021470723.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tight junction protein 1b (tjp1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470723.1

ORF Insert Sequence:

ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
TCCCGCAAGA ATGAAGCATG TATGGCGTCA GGAAGTTCTC AGAAGGTGAC ATCAGCAGAA 
TACGGGCTTC GCCTGGCCAG CCACATCTTC GTGAAGGACA TTTCCCCCGA GAGCCTGGCA 
GCCCGAGACG GAAACATCCA AGAGGGAGAC GTGGTGCTTA AGATTAATGG CACGGTAACA 
GAGAATTTGT CCCTGATCGA TGCTAAGAAG CTGATCGAGA GGTCAAAGGG AAAGCTGAAG 
ATGGTGGTGC AGAGAGACGA GCGGGCGACG CTGCTGAACA TCCCCGATCT GGACGACAGC 
ATACCTTCTG CTAATGCCTC GGACAGAGAT GATATTTCAG ACATCCACTC CGTGGCCTCT 
GATCATTCCA ACCGATCCCA CGACCGGAAG AGGAGCAGCC GCTCTCGTTC TCCAGACCGC 
CGCTCCGAAC CCTCAGACCA CTCCAGACAC TCTCCACCAC AGATCAGCAA CGGCAGGGCC 
ACAGGGCCGA CGGCTCAGAG AACGCGGCCA GTTCACAGGA GCCGTGATGA AGACAGGATC 
TCCAAGGCCG GGCCGCTTCC TCTACCTGCT AAGATGGTGG AGGAGATGCC GAAAGAGCAG 
AGCGCAGCCA GAGAAACAGA GAAACAGCTC CCCCCTCTGC CAGAGCCCAA ACCTGTGTAC 
GCTCAGCCGG GCCAGCCGGA CGTGGACCTG CCTGTTAGTC CGGCAGATGC TCCTGTGCCC 
AGCGTCACAC ATGATGACAG CATCTTACGG CCGAGCATGA AGCTGGTGAA GTTCAAGAAG 
GGCGAGAGCG TGGGCTTGAG GCTCGCTGGG GGGAACGACG TTGGGATATT TGTGGCGGGA 
GTTCTGGAGG ACAGTCCTGC TGCTAAAGAG GGCCTGGAAG AGGGAGACCA GATTCTCAGG 
GTAAATAATG TGGATTTTGC AAATATCATC CGAGAGGAGG CAGTGCTGTT CCTGCTGGAT 
CTGCCCAAAG GAGAGGAGGT CACCATCCTG GCCCAGAAGA AGAAAGACGT GTACCGCCGC 
ATTGTAGAGT CTGACGTGGG AGACTCGTTC TACATCAGGA CTCATTTTGA ATATGAGAAG 
GAGTCGCCCT ATGGCCTGAG CTTCAATAAG GGGGAGGTCT TCAGAGTCGT CGACACCCTT 
TACAATGGCA AACTGGGCTC CTGGCTCGCC ATCCGCATCG GCAAAAACCA CCAGGAGGTG 
GAGCGGGGCA TCATCCCCAA CAAGAACAGG GCTGAGCAGT TATCAAGTGT CCAGTACACT 
CTCCCAAAGA CTCCAGGAGG GGATCGCGCC GACTTCTGGA GATTCAGAGG CCTCCGCAGC 
TCCAAACGAA ACCTGAGGAA GAGCAGAGAA GACCTGTCTG CTCAGCCGGT CCAAACCAAA 
TTCCCTGCTT ATGAAAGAGT GGTGCTTAGA GAAGCTGGTT TCTTGAGGCC AGTGGTCATT 
TTCGGACCGA TCGCGGATGT GGCTAGAGAG AAGCTGTCCA GAGAGGAGAC TGACCTCTTC 
GAACTTGCAA AGAGTGAACC CAGAGATGCG GGCACAGACC AGCGCAGCTC CGGAATCATC 
CGTCTCCATA CCATTAAACA GATCATTGAC CGAGACAAGC ACGCGGTGCT GGACATCACC 
CCTAATGCGG TGGACCGTCT GAACTACGCT CAGTGGTACC CCATCGTGGT GTTTCTGAAT 
CCAGACAGCA AACAGGGCGT CAAGAACATG AGGACCAGGC TCTGTCCCGA GTCCAGGAAG 
AGCGCCCGAA AGCTCTACGA GCGTGCTCTC AAACTGAGGA AGAACAACCA TCACCTTTTC 
ACCACCACTA TTAACTTGAA CAACATGAAT GACGGGTGGT ACGGTGCTTT GAAAGAGAGC 
ATCCAGCAGC AGCAGAACCA GTTGGTCTGG GTCTCAGAGG GCAAGGCCGA CGGCGCTCCT 
GACGATGACC TTGACATCCA TGACGACCGC CTCTCCTACC TCTCGGCTCC GGGCAGCGAG 
TACAGCATGT ACAGCACCGA CAGCCGCCAC ACGTCCGACT ATGAGGACAC CGACACGGAG 
GGTGGTGCCT ACACCGACCA GGAGCTGGAC GAGACCATGA ACGATGAGGT GGGCCTGCCT 
AGCGAGCCTG CCATCACACG CTCATCTGAG CCTGTGCGAG AGGACCCGCC AGTCATTCAG 
GACGCAGCTG GATACCCCAG CTACCAGCAT GCGGTACCGC AACCTGAAGC GGTGAGCCGC 
ATTGACCCGG CCGGGTTTAA GATGGCAGCG CCGCAGCAGC AAGCTGAGGC TGCTCTGGCC 
TCGCCGCCCC CTCCCTCTGC AGCGGTCCAG CCCACCGCAA CACTAGCGGG TACGAACCCC 
GAGGAGCCGC CTGGCACCCC TCAGGCCGAC TCCCTTAACA GCCCCGTCCC TGTTCCCGAC 
CCCCAGCCCG AACCTGAGCT CGCTCAGCCC CCAACACACG ACCCCCACCA GTCGCTTCCT 
CCTGGCCCAG ATCCAAAGAT GTATAAAAAG GATCTGTACA GCATGGACGA GCCGGTGCGA 
GTGACCCACG GCGTGAAGCC TCCCCCTCCC CAGCAGCAGC AGCAGCCTTT AGGCCCCCCA 
ACCTCGCTCT CCTACAGCCA CCAGCCTGTC TACCAGGACC AACAGCCATA CCGCCAGTAC 
GACCACCCGC CTTATGGCTA TGACGGTGGC GGCTACGCAC AACCAAAGCC TCACAACTAC 
GACCCGCACC TGCACTACGA CAACCGTGTG CCTCACTATA ACGAGCAGTG GCCCACGTAC 
GACCAGCAGC AGCAGCAGCA GACTTCCCCC CAGCCGCCAC CTGCGTCCGG CTTCCCTCAG 
GGCCACCAGC CTCCGCCTCC ACCACCGATG GGATATGAGC CACGCTCCCC ATACGAGGAC 
GGACCTAACA GGGATTTCAG CCCTCCTCAG TCCCAGTATG ACGGTGTGTC CCCAATGGGC 
TACGATCACC GGCCGCGGCA CCCCAAACCT GCACCGGTCC GATATGAAGA GCCTCCACCT 
CCACCTCCTC CGGTGTCCTA CGATGCACGG TCACCTTTCG AACCCGAGCC GCATGCTTTC 
CCAGGCAACA CACATCGCTC GCCAGACCCA CCGAAACAGT ATTATGGAGA CGCCGCTATG 
AGGCCCTTGT ACAACCCTGG ACCTCCGAAC AGAGTGTACA AACATGAGCC GGTGAACTCT 
GAGCCTCCTG TACCGCCTCC CAAACCTGAA TCTGTCCTGT CTCCAGGAGA ACCGCCCCAT 
TCTGCAGCTT CCAAACCATT GCCGCCTCCT CCCAGAGATG ATGACGATGA TGATGACGAT 
GATGATGACC CTGCCATGAA GCCCCAGTCT GTGCTCAACA GGGTCAAAAT GTTCGAAAAC 
AAACGCTCTG TGTCGGTAGA CCGAGCCAAG GACGCACCTG ACGTCACTGG AATTAGGCCC 
ACAGATCTCC CCAAACCTGC GAGCACCCCT GGACCTGTGC TCAAAGCCAA CTCTCTCAGC 
AACCTGGAGC AGGACAAACC CTCCTACAGA GCTCCAGAGC CGCAGAAACC TCAGACGCGT 
GTTGGGGACG ATGTGGTTCG CTCCAACCAC TACGACCCTG ATGATGATGA AGAGTACTAC 
AGAAAACAGC TCTCATTTTT CGACCGCCGC AGTTTCGACA GTAAGGTCAT GAATCCACCC 
AGCGCTGGAG TAAACCGCTT CCATGAGCCG CCCAAACCAC CCCAGACACA GATCGCTTAT 
CCTTTCGCTA GGACGGAGTC TGTGGAGAAG GTGAGTCCTG TGGACAAGAG ATACGAGCCT 
CTGCCGCCAG TCAACCCTTC ACCTGCTCCA TACAGCCAGA ACACACCTGT CGCTCCGCCC 
ACATCCCTGC CCAAACTCAC CAACATCGAG GTGAACTCTC TGCCAGATCC TCACAGCTCT 
CCCAAAGCCA AACCGGACCT GTCTGCTCTG AGAGCTCCGA CCCGGGACGA GCCCATCCAG 
AGCAGCTACT TGCCCCCCAA GTCCACCATC AATGGCATGG ATGCTCCTCC CAAAACACTC 
GGCGTGCCGA CCAGCTACAA CCGCTACGTC CCCAAGCCTT ACACCAGCTC GGCGCGACCC 
TTCGAGCGCA AGTTCGAGAG TCCGAAATTC AACCACAACC TGCTTCCCAA CGACACGCAG 
TCCAAACCCA GTGTCAACAA CAACCTGAAG CCTCAGATCT CCCCACAACC CCAGGACACG 
GACAGCGGCG TCGACACCTT CACTCGCACC ATGGACAACA GGCCAAAATA TCAGCACAAC 
AACATCAACG CCATTCCCAA AGCCGTCCCA GTCAGCCCCA GTGCACTGGA TGATGATGAA 
GAAGATGAGG GACACACAGT TGTGGCCACG GCGCGGGGCA TCTTTAACTG TAATGGCGGG 
GTGTTGAGCT CTATAGAGAC GGGCGTCAGC ATCATTATCC CACAGGGAGC CATTCCTGAT 
AGTGTGGAGC AGGAGATCTA CTTCAAAGTG TGCCGGGACA ACAGCATCCT GCCTCCTCTG 
GATAAGGAGA AAGGTGAAAC TCTGCTGAGT CCGCTGGTGA TGTGTGGTCC TCACGGCCTG 
AAGTTCCTGA AGCCTGTGGA GCTGCGCTTG CCACACTGTG CGTCTATGAC CCCTGATGGT 
TGGTCTTTTG CTCTAAAATC CTCCGACTCC TCGTCGGGTG ACCCCAAATG CTGGCAAAAC 
AAGTGTCTAC CAGGAGACCC CAATTACCTG GTGGGCGCAA ACTGTGTGTC TGTGCTGATC 
GACCACTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp1b ( XM_021470725.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326400.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X3, mRNA.

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CloneID	ODa44617
Clone ID Related Accession (Same CDS sequence)	XM_021470725.1
Accession Version	XM_021470725.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6123bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tight junction protein ZO-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
TCCCGCAAGA ATGAAGCATG TATGGCGTCA GGAAGTTCTC AGAAGGTGAC ATCAGCAGAA 
TACGGGCTTC GCCTGGCCAG CCACATCTTC GTGAAGGACA TTTCCCCCGA GAGCCTGGCA 
GCCCGAGACG GAAACATCCA AGAGGGAGAC GTGGTGCTTA AGATTAATGG CACGGTAACA 
GAGAATTTGT CCCTGATCGA TGCTAAGAAG CTGATCGAGA GGTCAAAGGG AAAGCTGAAG 
ATGGTGGTGC AGAGAGACGA GCGGGCGACG CTGCTGAACA TCCCCGATCT GGACGACAGC 
ATACCTTCTG CTAATGCCTC GGACAGAGAT GACATCCACT CCGTGGCCTC TGATCATTCC 
AACCGATCCC ACGACCGGAA GAGGAGCAGC CGCTCTCGTT CTCCAGACCG CCGCTCCGAA 
CCCTCAGACC ACTCCAGACA CTCTCCACCA CAGATCAGCA ACGGCAGGGC CACAGGGCCG 
ACGGCTCAGA GAACGCGGCC AGTTCACAGG AGCCGTGATG AAGACAGGAT CTCCAAGGCC 
GGGCCGCTTC CTCTACCTGC TAAGATGGTG GAGGAGATGC CGAAAGAGCA GAGCGCAGCC 
AGAGAAACAG AGAAACAGCT CCCCCCTCTG CCAGAGCCCA AACCTGTGTA CGCTCAGCCG 
GGCCAGCCGG ACGTGGACCT GCCTGTTAGT CCGGCAGATG CTCCTGTGCC CAGCGTCACA 
CATGATGACA GCATCTTACG GCCGAGCATG AAGCTGGTGA AGTTCAAGAA GGGCGAGAGC 
GTGGGCTTGA GGCTCGCTGG GGGGAACGAC GTTGGGATAT TTGTGGCGGG AGTTCTGGAG 
GACAGTCCTG CTGCTAAAGA GGGCCTGGAA GAGGGAGACC AGATTCTCAG GGTAAATAAT 
GTGGATTTTG CAAATATCAT CCGAGAGGAG GCAGTGCTGT TCCTGCTGGA TCTGCCCAAA 
GGAGAGGAGG TCACCATCCT GGCCCAGAAG AAGAAAGACG TGTACCGCCG CATTGTAGAG 
TCTGACGTGG GAGACTCGTT CTACATCAGG ACTCATTTTG AATATGAGAA GGAGTCGCCC 
TATGGCCTGA GCTTCAATAA GGGGGAGGTC TTCAGAGTCG TCGACACCCT TTACAATGGC 
AAACTGGGCT CCTGGCTCGC CATCCGCATC GGCAAAAACC ACCAGGAGGT GGAGCGGGGC 
ATCATCCCCA ACAAGAACAG GGCTGAGCAG TTATCAAGTG TCCAGTACAC TCTCCCAAAG 
ACTCCAGGAG GGGATCGCGC CGACTTCTGG AGATTCAGAG GCCTCCGCAG CTCCAAACGA 
AACCTGAGGA AGAGCAGAGA AGACCTGTCT GCTCAGCCGG TCCAAACCAA ATTCCCTGCT 
TATGAAAGAG TGGTGCTTAG AGAAGCTGGT TTCTTGAGGC CAGTGGTCAT TTTCGGACCG 
ATCGCGGATG TGGCTAGAGA GAAGCTGTCC AGAGAGGAGA CTGACCTCTT CGAACTTGCA 
AAGAGTGAAC CCAGAGATGC GGGCACAGAC CAGCGCAGCT CCGGAATCAT CCGTCTCCAT 
ACCATTAAAC AGATCATTGA CCGAGACAAG CACGCGGTGC TGGACATCAC CCCTAATGCG 
GTGGACCGTC TGAACTACGC TCAGTGGTAC CCCATCGTGG TGTTTCTGAA TCCAGACAGC 
AAACAGGGCG TCAAGAACAT GAGGACCAGG CTCTGTCCCG AGTCCAGGAA GAGCGCCCGA 
AAGCTCTACG AGCGTGCTCT CAAACTGAGG AAGAACAACC ATCACCTTTT CACCACCACT 
ATTAACTTGA ACAACATGAA TGACGGGTGG TACGGTGCTT TGAAAGAGAG CATCCAGCAG 
CAGCAGAACC AGTTGGTCTG GGTCTCAGAG GGCAAGGCCG ACGGCGCTCC TGACGATGAC 
CTTGACATCC ATGACGACCG CCTCTCCTAC CTCTCGGCTC CGGGCAGCGA GTACAGCATG 
TACAGCACCG ACAGCCGCCA CACGTCCGAC TATGAGGACA CCGACACGGA GGGTGGTGCC 
TACACCGACC AGGAGCTGGA CGAGACCATG AACGATGAGG TGGGCCTGCC TAGCGAGCCT 
GCCATCACAC GCTCATCTGA GCCTGTGCGA GAGGACCCGC CAGTCATTCA GGACGCAGCT 
GGATACCCCA GCTACCAGCA TGCGGTACCG CAACCTGAAG CGGTGAGCCG CATTGACCCG 
GCCGGGTTTA AGATGGCAGC GCCGCAGCAG CAAGCTGAGG CTGCTCTGGC CTCGCCGCCC 
CCTCCCTCTG CAGCGGTCCA GCCCACCGCA ACACTAGCGG GTACGAACCC CGAGGAGCCG 
CCTGGCACCC CTCAGGCCGA CTCCCTTAAC AGCCCCGTCC CTGTTCCCGA CCCCCAGCCC 
GAACCTGAGC TCGCTCAGCC CCCAACACAC GACCCCCACC AGTCGCTTCC TCCTGGCCCA 
GATCCAAAGA TGTATAAAAA GGATCTGTAC AGCATGGACG AGCCGGTGCG AGTGACCCAC 
GGCGTGAAGC CTCCCCCTCC CCAGCAGCAG CAGCAGCCTT TAGGCCCCCC AACCTCGCTC 
TCCTACAGCC ACCAGCCTGT CTACCAGGAC CAACAGCCAT ACCGCCAGTA CGACCACCCG 
CCTTATGGCT ATGACGGTGG CGGCTACGCA CAACCAAAGC CTCACAACTA CGACCCGCAC 
CTGCACTACG ACAACCGTGT GCCTCACTAT AACGAGCAGT GGCCCACGTA CGACCAGCAG 
CAGCAGCAGC AGACTTCCCC CCAGCCGCCA CCTGCGTCCG GCTTCCCTCA GGGCCACCAG 
CCTCCGCCTC CACCACCGAT GGGATATGAG CCACGCTCCC CATACGAGGA CGGACCTAAC 
AGGGATTTCA GCCCTCCTCA GTCCCAGTAT GACGGTGTGT CCCCAATGGG CTACGATCAC 
CGGCCGCGGC ACCCCAAACC TGCACCGGTC CGATATGAAG AGCCTCCACC TCCACCTCCT 
CCGGTGTCCT ACGATGCACG GTCACCTTTC GAACCCGAGC CGCATGCTTT CCCAGGCAAC 
ACACATCGCT CGCCAGACCC ACCGAAACAG TATTATGGAG ACGCCGCTAT GAGGCCCTTG 
TACAACCCTG GACCTCCGAA CAGAGTGTAC AAACATGAGC CGGTGAACTC TGAGCCTCCT 
GTACCGCCTC CCAAACCTGA ATCTGTCCTG TCTCCAGGAG AACCGCCCCA TTCTGCAGCT 
TCCAAACCAT TGCCGCCTCC TCCCAGAGAT GATGACGATG ATGATGACGA TGATGATGAC 
CCTGCCATGA AGCCCCAGTC TGTGCTCAAC AGGGTCAAAA TGTTCGAAAA CAAACGCTCT 
GTGTCGGTAG ACCGAGCCAA GGACGCACCT GACGTCACTG GAATTAGGCC CACAGATCTC 
CCCAAACCTG CGAGCACCCC TGGACCTGTG CTCAAAGCCA ACTCTCTCAG CAACCTGGAG 
CAGGACAAAC CCTCCTACAG AGCTCCAGAG CCGCAGAAAC CTCAGACGCG TGTTGGGGAC 
GATGTGGTTC GCTCCAACCA CTACGACCCT GATGATGATG AAGAGTACTA CAGAAAACAG 
CTCTCATTTT TCGACCGCCG CAGTTTCGAC AGTAAGGTCA TGAATCCACC CAGCGCTGGA 
GTAAACCGCT TCCATGAGCC GCCCAAACCA CCCCAGACAC AGATCGCTTA TCCTTTCGCT 
AGGACGGAGT CTGTGGAGAA GGTGAGTCCT GTGGACAAGA GATACGAGCC TCTGCCGCCA 
GTCAACCCTT CACCTGCTCC ATACAGCCAG AACACACCTG TCGCTCCGCC CACATCCCTG 
CCCAAACTCA CCAACATCGA GGTGAACTCT CTGCCAGATC CTCACAGCTC TCCCAAAGCC 
AAACCGGACC TGTCTGCTCT GAGAGCTCCG ACCCGGGACG AGCCCATCCA GAGCAGCTAC 
TTGCCCCCCA AGTCCACCAT CAATGGCATG GATGCTCCTC CCAAAACACT CGGCGTGCCG 
ACCAGCTACA ACCGCTACGT CCCCAAGCCT TACACCAGCT CGGCGCGACC CTTCGAGCGC 
AAGTTCGAGA GTCCGAAATT CAACCACAAC CTGCTTCCCA ACGACACGCA GTCCAAACCC 
AGTGTCAACA ACAACCTGAA GCCTCAGATC TCCCCACAAC CCCAGGACAC GGACAGCGGC 
GTCGACACCT TCACTCGCAC CATGGACAAC AGGCCAAAAT ATCAGCACAA CAACATCAAC 
GCCATTCCCA AAGCCGTCCC AGTCAGCCCC AGTGCACTGG ATGATGATGA AGAAGATGAG 
GGACACACAG TTGTGGCCAC GGCGCGGGGC ATCTTTAACT GTAATGGCGG GGTGTTGAGC 
TCTATAGAGA CGGGCGTCAG CATCATTATC CCACAGGGAG CCATTCCTGA TAGTGTGGAG 
CAGGAGATCT ACTTCAAAGT GTGCCGGGAC AACAGCATCC TGCCTCCTCT GGATAAGGAG 
AAAGGTGAAA CTCTGCTGAG TCCGCTGGTG ATGTGTGGTC CTCACGGCCT GAAGTTCCTG 
AAGCCTGTGG AGCTGCGCTT GCCACACTGT GCGTCTATGA CCCCTGATGG TTGGTCTTTT 
GCTCTAAAAT CCTCCGACTC CTCGTCGGGT GACCCCAAAT GCTGGCAAAA CAAGTGTCTA 
CCAGGAGACC CCAATTACCT GGTGGGCGCA AACTGTGTGT CTGTGCTGAT CGACCACTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326400.1
CDS	110..6232
Translation	MLLSVFGGPVEEVLAGLLKLKMFGSGSKKWFIKKNKRQRTKVQRKPASGRRNKRVSRVLKRHKEVIVKQLDVSKVLPTLVYERVFTLGEYKEILGQDSSGKRAELFLDLLSQKEPAAFSAFCGVLEEVCPHLLTCFLLDGTDGSAGENKKQGQTKPAHSQQNALSKGQDTVCFPPMYTDPLFDTRVFPKHDKDKYGPAVYYPKFNAYIPNPDKVSVLDESEGSGHGKPSLRRIKGRIHRSKSLDSIDLLDSNSAAMDETVIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGLLQENDRVVMVNAVSMDNVDHAYAVQQLRKSGKNAKITIRRKRKVQIPSGRPGERETMSEHDEDDDSYEDEIYESRSARSGPSAYSGGGGGGGGGGASGRRSGRGDRLSGRRERDRERSGSRERSLSPRSDRHSVSSNLPPRPAKVTLVKSRKNEACMASGSSQKVTSAEYGLRLASHIFVKDISPESLAARDGNIQEGDVVLKINGTVTENLSLIDAKKLIERSKGKLKMVVQRDERATLLNIPDLDDSIPSANASDRDDIHSVASDHSNRSHDRKRSSRSRSPDRRSEPSDHSRHSPPQISNGRATGPTAQRTRPVHRSRDEDRISKAGPLPLPAKMVEEMPKEQSAARETEKQLPPLPEPKPVYAQPGQPDVDLPVSPADAPVPSVTHDDSILRPSMKLVKFKKGESVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFANIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHQEVERGIIPNKNRAEQLSSVQYTLPKTPGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVVIFGPIADVAREKLSREETDLFELAKSEPRDAGTDQRSSGIIRLHTIKQIIDRDKHAVLDITPNAVDRLNYAQWYPIVVFLNPDSKQGVKNMRTRLCPESRKSARKLYERALKLRKNNHHLFTTTINLNNMNDGWYGALKESIQQQQNQLVWVSEGKADGAPDDDLDIHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETMNDEVGLPSEPAITRSSEPVREDPPVIQDAAGYPSYQHAVPQPEAVSRIDPAGFKMAAPQQQAEAALASPPPPSAAVQPTATLAGTNPEEPPGTPQADSLNSPVPVPDPQPEPELAQPPTHDPHQSLPPGPDPKMYKKDLYSMDEPVRVTHGVKPPPPQQQQQPLGPPTSLSYSHQPVYQDQQPYRQYDHPPYGYDGGGYAQPKPHNYDPHLHYDNRVPHYNEQWPTYDQQQQQQTSPQPPPASGFPQGHQPPPPPPMGYEPRSPYEDGPNRDFSPPQSQYDGVSPMGYDHRPRHPKPAPVRYEEPPPPPPPVSYDARSPFEPEPHAFPGNTHRSPDPPKQYYGDAAMRPLYNPGPPNRVYKHEPVNSEPPVPPPKPESVLSPGEPPHSAASKPLPPPPRDDDDDDDDDDDPAMKPQSVLNRVKMFENKRSVSVDRAKDAPDVTGIRPTDLPKPASTPGPVLKANSLSNLEQDKPSYRAPEPQKPQTRVGDDVVRSNHYDPDDDEEYYRKQLSFFDRRSFDSKVMNPPSAGVNRFHEPPKPPQTQIAYPFARTESVEKVSPVDKRYEPLPPVNPSPAPYSQNTPVAPPTSLPKLTNIEVNSLPDPHSSPKAKPDLSALRAPTRDEPIQSSYLPPKSTINGMDAPPKTLGVPTSYNRYVPKPYTSSARPFERKFESPKFNHNLLPNDTQSKPSVNNNLKPQISPQPQDTDSGVDTFTRTMDNRPKYQHNNINAIPKAVPVSPSALDDDEEDEGHTVVATARGIFNCNGGVLSSIETGVSIIIPQGAIPDSVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKCWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_021470725.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tight junction protein 1b (tjp1b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470725.1

ORF Insert Sequence:

ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
TCCCGCAAGA ATGAAGCATG TATGGCGTCA GGAAGTTCTC AGAAGGTGAC ATCAGCAGAA 
TACGGGCTTC GCCTGGCCAG CCACATCTTC GTGAAGGACA TTTCCCCCGA GAGCCTGGCA 
GCCCGAGACG GAAACATCCA AGAGGGAGAC GTGGTGCTTA AGATTAATGG CACGGTAACA 
GAGAATTTGT CCCTGATCGA TGCTAAGAAG CTGATCGAGA GGTCAAAGGG AAAGCTGAAG 
ATGGTGGTGC AGAGAGACGA GCGGGCGACG CTGCTGAACA TCCCCGATCT GGACGACAGC 
ATACCTTCTG CTAATGCCTC GGACAGAGAT GACATCCACT CCGTGGCCTC TGATCATTCC 
AACCGATCCC ACGACCGGAA GAGGAGCAGC CGCTCTCGTT CTCCAGACCG CCGCTCCGAA 
CCCTCAGACC ACTCCAGACA CTCTCCACCA CAGATCAGCA ACGGCAGGGC CACAGGGCCG 
ACGGCTCAGA GAACGCGGCC AGTTCACAGG AGCCGTGATG AAGACAGGAT CTCCAAGGCC 
GGGCCGCTTC CTCTACCTGC TAAGATGGTG GAGGAGATGC CGAAAGAGCA GAGCGCAGCC 
AGAGAAACAG AGAAACAGCT CCCCCCTCTG CCAGAGCCCA AACCTGTGTA CGCTCAGCCG 
GGCCAGCCGG ACGTGGACCT GCCTGTTAGT CCGGCAGATG CTCCTGTGCC CAGCGTCACA 
CATGATGACA GCATCTTACG GCCGAGCATG AAGCTGGTGA AGTTCAAGAA GGGCGAGAGC 
GTGGGCTTGA GGCTCGCTGG GGGGAACGAC GTTGGGATAT TTGTGGCGGG AGTTCTGGAG 
GACAGTCCTG CTGCTAAAGA GGGCCTGGAA GAGGGAGACC AGATTCTCAG GGTAAATAAT 
GTGGATTTTG CAAATATCAT CCGAGAGGAG GCAGTGCTGT TCCTGCTGGA TCTGCCCAAA 
GGAGAGGAGG TCACCATCCT GGCCCAGAAG AAGAAAGACG TGTACCGCCG CATTGTAGAG 
TCTGACGTGG GAGACTCGTT CTACATCAGG ACTCATTTTG AATATGAGAA GGAGTCGCCC 
TATGGCCTGA GCTTCAATAA GGGGGAGGTC TTCAGAGTCG TCGACACCCT TTACAATGGC 
AAACTGGGCT CCTGGCTCGC CATCCGCATC GGCAAAAACC ACCAGGAGGT GGAGCGGGGC 
ATCATCCCCA ACAAGAACAG GGCTGAGCAG TTATCAAGTG TCCAGTACAC TCTCCCAAAG 
ACTCCAGGAG GGGATCGCGC CGACTTCTGG AGATTCAGAG GCCTCCGCAG CTCCAAACGA 
AACCTGAGGA AGAGCAGAGA AGACCTGTCT GCTCAGCCGG TCCAAACCAA ATTCCCTGCT 
TATGAAAGAG TGGTGCTTAG AGAAGCTGGT TTCTTGAGGC CAGTGGTCAT TTTCGGACCG 
ATCGCGGATG TGGCTAGAGA GAAGCTGTCC AGAGAGGAGA CTGACCTCTT CGAACTTGCA 
AAGAGTGAAC CCAGAGATGC GGGCACAGAC CAGCGCAGCT CCGGAATCAT CCGTCTCCAT 
ACCATTAAAC AGATCATTGA CCGAGACAAG CACGCGGTGC TGGACATCAC CCCTAATGCG 
GTGGACCGTC TGAACTACGC TCAGTGGTAC CCCATCGTGG TGTTTCTGAA TCCAGACAGC 
AAACAGGGCG TCAAGAACAT GAGGACCAGG CTCTGTCCCG AGTCCAGGAA GAGCGCCCGA 
AAGCTCTACG AGCGTGCTCT CAAACTGAGG AAGAACAACC ATCACCTTTT CACCACCACT 
ATTAACTTGA ACAACATGAA TGACGGGTGG TACGGTGCTT TGAAAGAGAG CATCCAGCAG 
CAGCAGAACC AGTTGGTCTG GGTCTCAGAG GGCAAGGCCG ACGGCGCTCC TGACGATGAC 
CTTGACATCC ATGACGACCG CCTCTCCTAC CTCTCGGCTC CGGGCAGCGA GTACAGCATG 
TACAGCACCG ACAGCCGCCA CACGTCCGAC TATGAGGACA CCGACACGGA GGGTGGTGCC 
TACACCGACC AGGAGCTGGA CGAGACCATG AACGATGAGG TGGGCCTGCC TAGCGAGCCT 
GCCATCACAC GCTCATCTGA GCCTGTGCGA GAGGACCCGC CAGTCATTCA GGACGCAGCT 
GGATACCCCA GCTACCAGCA TGCGGTACCG CAACCTGAAG CGGTGAGCCG CATTGACCCG 
GCCGGGTTTA AGATGGCAGC GCCGCAGCAG CAAGCTGAGG CTGCTCTGGC CTCGCCGCCC 
CCTCCCTCTG CAGCGGTCCA GCCCACCGCA ACACTAGCGG GTACGAACCC CGAGGAGCCG 
CCTGGCACCC CTCAGGCCGA CTCCCTTAAC AGCCCCGTCC CTGTTCCCGA CCCCCAGCCC 
GAACCTGAGC TCGCTCAGCC CCCAACACAC GACCCCCACC AGTCGCTTCC TCCTGGCCCA 
GATCCAAAGA TGTATAAAAA GGATCTGTAC AGCATGGACG AGCCGGTGCG AGTGACCCAC 
GGCGTGAAGC CTCCCCCTCC CCAGCAGCAG CAGCAGCCTT TAGGCCCCCC AACCTCGCTC 
TCCTACAGCC ACCAGCCTGT CTACCAGGAC CAACAGCCAT ACCGCCAGTA CGACCACCCG 
CCTTATGGCT ATGACGGTGG CGGCTACGCA CAACCAAAGC CTCACAACTA CGACCCGCAC 
CTGCACTACG ACAACCGTGT GCCTCACTAT AACGAGCAGT GGCCCACGTA CGACCAGCAG 
CAGCAGCAGC AGACTTCCCC CCAGCCGCCA CCTGCGTCCG GCTTCCCTCA GGGCCACCAG 
CCTCCGCCTC CACCACCGAT GGGATATGAG CCACGCTCCC CATACGAGGA CGGACCTAAC 
AGGGATTTCA GCCCTCCTCA GTCCCAGTAT GACGGTGTGT CCCCAATGGG CTACGATCAC 
CGGCCGCGGC ACCCCAAACC TGCACCGGTC CGATATGAAG AGCCTCCACC TCCACCTCCT 
CCGGTGTCCT ACGATGCACG GTCACCTTTC GAACCCGAGC CGCATGCTTT CCCAGGCAAC 
ACACATCGCT CGCCAGACCC ACCGAAACAG TATTATGGAG ACGCCGCTAT GAGGCCCTTG 
TACAACCCTG GACCTCCGAA CAGAGTGTAC AAACATGAGC CGGTGAACTC TGAGCCTCCT 
GTACCGCCTC CCAAACCTGA ATCTGTCCTG TCTCCAGGAG AACCGCCCCA TTCTGCAGCT 
TCCAAACCAT TGCCGCCTCC TCCCAGAGAT GATGACGATG ATGATGACGA TGATGATGAC 
CCTGCCATGA AGCCCCAGTC TGTGCTCAAC AGGGTCAAAA TGTTCGAAAA CAAACGCTCT 
GTGTCGGTAG ACCGAGCCAA GGACGCACCT GACGTCACTG GAATTAGGCC CACAGATCTC 
CCCAAACCTG CGAGCACCCC TGGACCTGTG CTCAAAGCCA ACTCTCTCAG CAACCTGGAG 
CAGGACAAAC CCTCCTACAG AGCTCCAGAG CCGCAGAAAC CTCAGACGCG TGTTGGGGAC 
GATGTGGTTC GCTCCAACCA CTACGACCCT GATGATGATG AAGAGTACTA CAGAAAACAG 
CTCTCATTTT TCGACCGCCG CAGTTTCGAC AGTAAGGTCA TGAATCCACC CAGCGCTGGA 
GTAAACCGCT TCCATGAGCC GCCCAAACCA CCCCAGACAC AGATCGCTTA TCCTTTCGCT 
AGGACGGAGT CTGTGGAGAA GGTGAGTCCT GTGGACAAGA GATACGAGCC TCTGCCGCCA 
GTCAACCCTT CACCTGCTCC ATACAGCCAG AACACACCTG TCGCTCCGCC CACATCCCTG 
CCCAAACTCA CCAACATCGA GGTGAACTCT CTGCCAGATC CTCACAGCTC TCCCAAAGCC 
AAACCGGACC TGTCTGCTCT GAGAGCTCCG ACCCGGGACG AGCCCATCCA GAGCAGCTAC 
TTGCCCCCCA AGTCCACCAT CAATGGCATG GATGCTCCTC CCAAAACACT CGGCGTGCCG 
ACCAGCTACA ACCGCTACGT CCCCAAGCCT TACACCAGCT CGGCGCGACC CTTCGAGCGC 
AAGTTCGAGA GTCCGAAATT CAACCACAAC CTGCTTCCCA ACGACACGCA GTCCAAACCC 
AGTGTCAACA ACAACCTGAA GCCTCAGATC TCCCCACAAC CCCAGGACAC GGACAGCGGC 
GTCGACACCT TCACTCGCAC CATGGACAAC AGGCCAAAAT ATCAGCACAA CAACATCAAC 
GCCATTCCCA AAGCCGTCCC AGTCAGCCCC AGTGCACTGG ATGATGATGA AGAAGATGAG 
GGACACACAG TTGTGGCCAC GGCGCGGGGC ATCTTTAACT GTAATGGCGG GGTGTTGAGC 
TCTATAGAGA CGGGCGTCAG CATCATTATC CCACAGGGAG CCATTCCTGA TAGTGTGGAG 
CAGGAGATCT ACTTCAAAGT GTGCCGGGAC AACAGCATCC TGCCTCCTCT GGATAAGGAG 
AAAGGTGAAA CTCTGCTGAG TCCGCTGGTG ATGTGTGGTC CTCACGGCCT GAAGTTCCTG 
AAGCCTGTGG AGCTGCGCTT GCCACACTGT GCGTCTATGA CCCCTGATGG TTGGTCTTTT 
GCTCTAAAAT CCTCCGACTC CTCGTCGGGT GACCCCAAAT GCTGGCAAAA CAAGTGTCTA 
CCAGGAGACC CCAATTACCT GGTGGGCGCA AACTGTGTGT CTGTGCTGAT CGACCACTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp1b ( XM_021470726.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326401.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X4, mRNA.

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CloneID	ODa44618
Clone ID Related Accession (Same CDS sequence)	XM_021470726.1
Accession Version	XM_021470726.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6117bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tight junction protein ZO-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
TCCCGCAAGA ATGAAGCATG TATGGCGTCA GGAAGTTCTC AGAAGGTGAC ATCAGCAGAA 
TACGGGCTTC GCCTGGCCAG CCACATCTTC GTGAAGGACA TTTCCCCCGA GAGCCTGGCA 
GCCCGAGACG GAAACATCCA AGAGGGAGAC GTGGTGCTTA AGATTAATGG CACGGTAACA 
GAGAATTTGT CCCTGATCGA TGCTAAGAAG CTGATCGAGA GGTCAAAGGG AAAGCTGAAG 
ATGGTGGTGC AGAGAGACGA GCGGGCGACG CTGCTGAACA TCCCCGATCT GGACGACAGC 
ATACCTTCTG CTAATGCCTC GGACAGAGAT GATATTTCAG ACATCCACTC CGTGGCCTCT 
GATCATTCCA ACCGATCCCA CGACCGGAAG AGGAGCAGCC GCTCTCGTTC TCCAGACCGC 
CGCTCCGAAC CCTCAGACCA CTCCAGACAC TCTCCACCAC AGATCAGCAA CGGCAGGGCC 
ACAGGGCCGA CGGCTCAGAG AACGCGGCCA GTTCACAGGA GCCGTGATGA AGACAGGATC 
TCCAAGGCCG GGCCGCTTCC TCTACCTGCT AAGATGGTGG AGGAGATGCC GAAAGAGCAG 
AGCGCAGCCA GAGAAACAGA GAAACAGCTC CCCCCTCTGC CAGAGCCCAA ACCTGTGTAC 
GCTCAGCCGG GCCAGCCGGA CGTGGACCTG CCTGTTAGTC CGGCAGATGC TCCTGTGCCC 
AGCGTCACAC ATGATGACAG CATCTTACGG CCGAGCATGA AGCTGGTGAA GTTCAAGAAG 
GGCGAGAGCG TGGGCTTGAG GCTCGCTGGG GGGAACGACG TTGGGATATT TGTGGCGGGA 
GTTCTGGAGG ACAGTCCTGC TGCTAAAGAG GGCCTGGAAG AGGGAGACCA GATTCTCAGG 
GTAAATAATG TGGATTTTGC AAATATCATC CGAGAGGAGG CAGTGCTGTT CCTGCTGGAT 
CTGCCCAAAG GAGAGGAGGT CACCATCCTG GCCCAGAAGA AGAAAGACGT GTACCGCCGC 
ATTGTAGAGT CTGACGTGGG AGACTCGTTC TACATCAGGA CTCATTTTGA ATATGAGAAG 
GAGTCGCCCT ATGGCCTGAG CTTCAATAAG GGGGAGGTCT TCAGAGTCGT CGACACCCTT 
TACAATGGCA AACTGGGCTC CTGGCTCGCC ATCCGCATCG GCAAAAACCA CCAGGAGGTG 
GAGCGGGGCA TCATCCCCAA CAAGAACAGG GCTGAGCAGT TATCAAGTGT CCAGTACACT 
CTCCCAAAGA CTCCAGGAGG GGATCGCGCC GACTTCTGGA GATTCAGAGG CCTCCGCAGC 
TCCAAACGAA ACCTGAGGAA GAGCAGAGAA GACCTGTCTG CTCAGCCGGT CCAAACCAAA 
TTCCCTGCTT ATGAAAGAGT GGTGCTTAGA GAAGCTGGTT TCTTGAGGCC AGTGGTCATT 
TTCGGACCGA TCGCGGATGT GGCTAGAGAG AAGCTGTCCA GAGAGGAGAC TGACCTCTTC 
GAACTTGCAA AGAGTGAACC CAGAGATGCG GGCACAGACC AGCGCAGCTC CGGAATCATC 
CGTCTCCATA CCATTAAACA GATCATTGAC CGAGACAAGC ACGCGGTGCT GGACATCACC 
CCTAATGCGG TGGACCGTCT GAACTACGCT CAGTGGTACC CCATCGTGGT GTTTCTGAAT 
CCAGACAGCA AACAGGGCGT CAAGAACATG AGGACCAGGC TCTGTCCCGA GTCCAGGAAG 
AGCGCCCGAA AGCTCTACGA GCGTGCTCTC AAACTGAGGA AGAACAACCA TCACCTTTTC 
ACCACCACTA TTAACTTGAA CAACATGAAT GACGGGTGGT ACGGTGCTTT GAAAGAGAGC 
ATCCAGCAGC AGCAGAACCA GTTGGTCTGG GTCTCAGAGG GCAAGGCCGA CGGCGCTCCT 
GACGATGACC TTGACATCCA TGACGACCGC CTCTCCTACC TCTCGGCTCC GGGCAGCGAG 
TACAGCATGT ACAGCACCGA CAGCCGCCAC ACGTCCGACT ATGAGGACAC CGACACGGAG 
GGTGGTGCCT ACACCGACCA GGAGCTGGAC GAGACCATGA ACGATGAGGT GGGCCTGCCT 
AGCGAGCCTG CCATCACACG CTCATCTGAG CCTGTGCGAG AGGACCCGCC AGTCATTCAG 
GACGCAGCTG GATACCCCAG CTACCAGCAT GCGGTACCGC AACCTGAAGC GGTGAGCCGC 
ATTGACCCGG CCGGGTTTAA GATGGCAGCG CCGCAGCAGC AAGCTGAGGC TGCTCTGGCC 
TCGCCGCCCC CTCCCTCTGC AGCGGTCCAG CCCACCGCAA CACTAGCGGG TACGAACCCC 
GAGGAGCCGC CTGGCACCCC TCAGGCCGAC TCCCTTAACA GCCCCGTCCC TGTTCCCGAC 
CCCCAGCCCG AACCTGAGCT CGCTCAGCCC CCAACACACG ACCCCCACCA GTCGCTTCCT 
CCTGGCCCAG ATCCAAAGAT GTATAAAAAG GATCTGTACA GCATGGACGA GCCGGTGCGA 
GTGACCCACG GCGTGAAGCC TCCCCCTCCC CAGCAGCAGC AGCAGCCTTT AGGCCCCCCA 
ACCTCGCTCT CCTACAGCCA CCAGCCTGTC TACCAGGACC AACAGCCATA CCGCCAGTAC 
GACCACCCGC CTTATGGCTA TGACGGTGGC GGCTACGCAC AACCAAAGCC TCACAACTAC 
GACCCGCACC TGCACTACGA CAACCGTGTG CCTCACTATA ACGAGCAGTG GCCCACGTAC 
GACCAGCAGC AGCAGCAGCA GACTTCCCCC CAGCCGCCAC CTGCGTCCGG CTTCCCTCAG 
GGCCACCAGC CTCCGCCTCC ACCACCGATG GGATATGAGC CACGCTCCCC ATACGAGGAC 
GGACCTAACA GGGATTTCAG CCCTCCTCAG TCCCAGTATG ACGGTGTGTC CCCAATGGGC 
TACGATCACC GGCCGCGGCA CCCCAAACCT GCACCGGTCC GATATGAAGA GCCTCCACCT 
CCACCTCCTC CGGTGTCCTA CGATGCACGG TCACCTTTCG AACCCGAGCC GCATGCTTTC 
CCAGGCAACA CACATCGCTC GCCAGACCCA CCGAAACAGT ATTATGGAGA CGCCGCTATG 
AGGCCCTTGT ACAACCCTGG ACCTCCGAAC AGAGTGTACA AACATGAGCC GGTGAACTCT 
GAGCCTCCTG TACCGCCTCC CAAACCTGAA TCTGTCCTGT CTCCAGGAGA ACCGCCCCAT 
TCTGCAGCTT CCAAACCATT GCCGCCTCCT CCCAGAGATG ATGACGATGA TGATGACGAT 
GATGATGACC CTGCCATGAA GCCCCAGTCT GTGCTCAACA GGGTCAAAAT GTTCGAAAAC 
AAACGCTCTG TGTCGGTAGA CCGAGCCAAG GACGCACCTG ACGTCACTGG AATTAGGCCC 
ACAGATCTCC CCAAACCTGC GAGCACCCCT GGACCTGTGC TCAAAGCCAA CTCTCTCAGC 
AACCTGGAGC AGGACAAACC CTCCTACAGA GCTCCAGAGC CGCAGAAACC TCAGACGCGT 
GTTGGGGACG ATGTGGTTCG CTCCAACCAC TACGACCCTG ATGATGATGA AGAGTACTAC 
AGAAAACAGC TCTCATTTTT CGACCGCCGC AGTTTCGACA GTAAGGTCAT GAATCCACCC 
AGCGCTGGAG TAAACCGCTT CCATGAGCCG CCCAAACCAC CCCAGACACA GATCGCTTAT 
CCTTTCGCTA GGACGGAGTC TGTGGAGAAG GTGAGTCCTG TGGACAAGAG ATACGAGCCT 
CTGCCGCCAG TCAACCCTTC ACCTGCTCCA TACAGCCAGA ACACACCTGT CGCTCCGCCC 
ACATCCCTGC CCAAACTCAC CAACATCGAG GATCCTCACA GCTCTCCCAA AGCCAAACCG 
GACCTGTCTG CTCTGAGAGC TCCGACCCGG GACGAGCCCA TCCAGAGCAG CTACTTGCCC 
CCCAAGTCCA CCATCAATGG CATGGATGCT CCTCCCAAAA CACTCGGCGT GCCGACCAGC 
TACAACCGCT ACGTCCCCAA GCCTTACACC AGCTCGGCGC GACCCTTCGA GCGCAAGTTC 
GAGAGTCCGA AATTCAACCA CAACCTGCTT CCCAACGACA CGCAGTCCAA ACCCAGTGTC 
AACAACAACC TGAAGCCTCA GATCTCCCCA CAACCCCAGG ACACGGACAG CGGCGTCGAC 
ACCTTCACTC GCACCATGGA CAACAGGCCA AAATATCAGC ACAACAACAT CAACGCCATT 
CCCAAAGCCG TCCCAGTCAG CCCCAGTGCA CTGGATGATG ATGAAGAAGA TGAGGGACAC 
ACAGTTGTGG CCACGGCGCG GGGCATCTTT AACTGTAATG GCGGGGTGTT GAGCTCTATA 
GAGACGGGCG TCAGCATCAT TATCCCACAG GGAGCCATTC CTGATAGTGT GGAGCAGGAG 
ATCTACTTCA AAGTGTGCCG GGACAACAGC ATCCTGCCTC CTCTGGATAA GGAGAAAGGT 
GAAACTCTGC TGAGTCCGCT GGTGATGTGT GGTCCTCACG GCCTGAAGTT CCTGAAGCCT 
GTGGAGCTGC GCTTGCCACA CTGTGCGTCT ATGACCCCTG ATGGTTGGTC TTTTGCTCTA 
AAATCCTCCG ACTCCTCGTC GGGTGACCCC AAATGCTGGC AAAACAAGTG TCTACCAGGA 
GACCCCAATT ACCTGGTGGG CGCAAACTGT GTGTCTGTGC TGATCGACCA CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326401.1
CDS	110..6226
Translation	MLLSVFGGPVEEVLAGLLKLKMFGSGSKKWFIKKNKRQRTKVQRKPASGRRNKRVSRVLKRHKEVIVKQLDVSKVLPTLVYERVFTLGEYKEILGQDSSGKRAELFLDLLSQKEPAAFSAFCGVLEEVCPHLLTCFLLDGTDGSAGENKKQGQTKPAHSQQNALSKGQDTVCFPPMYTDPLFDTRVFPKHDKDKYGPAVYYPKFNAYIPNPDKVSVLDESEGSGHGKPSLRRIKGRIHRSKSLDSIDLLDSNSAAMDETVIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGLLQENDRVVMVNAVSMDNVDHAYAVQQLRKSGKNAKITIRRKRKVQIPSGRPGERETMSEHDEDDDSYEDEIYESRSARSGPSAYSGGGGGGGGGGASGRRSGRGDRLSGRRERDRERSGSRERSLSPRSDRHSVSSNLPPRPAKVTLVKSRKNEACMASGSSQKVTSAEYGLRLASHIFVKDISPESLAARDGNIQEGDVVLKINGTVTENLSLIDAKKLIERSKGKLKMVVQRDERATLLNIPDLDDSIPSANASDRDDISDIHSVASDHSNRSHDRKRSSRSRSPDRRSEPSDHSRHSPPQISNGRATGPTAQRTRPVHRSRDEDRISKAGPLPLPAKMVEEMPKEQSAARETEKQLPPLPEPKPVYAQPGQPDVDLPVSPADAPVPSVTHDDSILRPSMKLVKFKKGESVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFANIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHQEVERGIIPNKNRAEQLSSVQYTLPKTPGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVVIFGPIADVAREKLSREETDLFELAKSEPRDAGTDQRSSGIIRLHTIKQIIDRDKHAVLDITPNAVDRLNYAQWYPIVVFLNPDSKQGVKNMRTRLCPESRKSARKLYERALKLRKNNHHLFTTTINLNNMNDGWYGALKESIQQQQNQLVWVSEGKADGAPDDDLDIHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETMNDEVGLPSEPAITRSSEPVREDPPVIQDAAGYPSYQHAVPQPEAVSRIDPAGFKMAAPQQQAEAALASPPPPSAAVQPTATLAGTNPEEPPGTPQADSLNSPVPVPDPQPEPELAQPPTHDPHQSLPPGPDPKMYKKDLYSMDEPVRVTHGVKPPPPQQQQQPLGPPTSLSYSHQPVYQDQQPYRQYDHPPYGYDGGGYAQPKPHNYDPHLHYDNRVPHYNEQWPTYDQQQQQQTSPQPPPASGFPQGHQPPPPPPMGYEPRSPYEDGPNRDFSPPQSQYDGVSPMGYDHRPRHPKPAPVRYEEPPPPPPPVSYDARSPFEPEPHAFPGNTHRSPDPPKQYYGDAAMRPLYNPGPPNRVYKHEPVNSEPPVPPPKPESVLSPGEPPHSAASKPLPPPPRDDDDDDDDDDDPAMKPQSVLNRVKMFENKRSVSVDRAKDAPDVTGIRPTDLPKPASTPGPVLKANSLSNLEQDKPSYRAPEPQKPQTRVGDDVVRSNHYDPDDDEEYYRKQLSFFDRRSFDSKVMNPPSAGVNRFHEPPKPPQTQIAYPFARTESVEKVSPVDKRYEPLPPVNPSPAPYSQNTPVAPPTSLPKLTNIEDPHSSPKAKPDLSALRAPTRDEPIQSSYLPPKSTINGMDAPPKTLGVPTSYNRYVPKPYTSSARPFERKFESPKFNHNLLPNDTQSKPSVNNNLKPQISPQPQDTDSGVDTFTRTMDNRPKYQHNNINAIPKAVPVSPSALDDDEEDEGHTVVATARGIFNCNGGVLSSIETGVSIIIPQGAIPDSVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKCWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_021470726.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tight junction protein 1b (tjp1b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470726.1

ORF Insert Sequence:

ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
TCCCGCAAGA ATGAAGCATG TATGGCGTCA GGAAGTTCTC AGAAGGTGAC ATCAGCAGAA 
TACGGGCTTC GCCTGGCCAG CCACATCTTC GTGAAGGACA TTTCCCCCGA GAGCCTGGCA 
GCCCGAGACG GAAACATCCA AGAGGGAGAC GTGGTGCTTA AGATTAATGG CACGGTAACA 
GAGAATTTGT CCCTGATCGA TGCTAAGAAG CTGATCGAGA GGTCAAAGGG AAAGCTGAAG 
ATGGTGGTGC AGAGAGACGA GCGGGCGACG CTGCTGAACA TCCCCGATCT GGACGACAGC 
ATACCTTCTG CTAATGCCTC GGACAGAGAT GATATTTCAG ACATCCACTC CGTGGCCTCT 
GATCATTCCA ACCGATCCCA CGACCGGAAG AGGAGCAGCC GCTCTCGTTC TCCAGACCGC 
CGCTCCGAAC CCTCAGACCA CTCCAGACAC TCTCCACCAC AGATCAGCAA CGGCAGGGCC 
ACAGGGCCGA CGGCTCAGAG AACGCGGCCA GTTCACAGGA GCCGTGATGA AGACAGGATC 
TCCAAGGCCG GGCCGCTTCC TCTACCTGCT AAGATGGTGG AGGAGATGCC GAAAGAGCAG 
AGCGCAGCCA GAGAAACAGA GAAACAGCTC CCCCCTCTGC CAGAGCCCAA ACCTGTGTAC 
GCTCAGCCGG GCCAGCCGGA CGTGGACCTG CCTGTTAGTC CGGCAGATGC TCCTGTGCCC 
AGCGTCACAC ATGATGACAG CATCTTACGG CCGAGCATGA AGCTGGTGAA GTTCAAGAAG 
GGCGAGAGCG TGGGCTTGAG GCTCGCTGGG GGGAACGACG TTGGGATATT TGTGGCGGGA 
GTTCTGGAGG ACAGTCCTGC TGCTAAAGAG GGCCTGGAAG AGGGAGACCA GATTCTCAGG 
GTAAATAATG TGGATTTTGC AAATATCATC CGAGAGGAGG CAGTGCTGTT CCTGCTGGAT 
CTGCCCAAAG GAGAGGAGGT CACCATCCTG GCCCAGAAGA AGAAAGACGT GTACCGCCGC 
ATTGTAGAGT CTGACGTGGG AGACTCGTTC TACATCAGGA CTCATTTTGA ATATGAGAAG 
GAGTCGCCCT ATGGCCTGAG CTTCAATAAG GGGGAGGTCT TCAGAGTCGT CGACACCCTT 
TACAATGGCA AACTGGGCTC CTGGCTCGCC ATCCGCATCG GCAAAAACCA CCAGGAGGTG 
GAGCGGGGCA TCATCCCCAA CAAGAACAGG GCTGAGCAGT TATCAAGTGT CCAGTACACT 
CTCCCAAAGA CTCCAGGAGG GGATCGCGCC GACTTCTGGA GATTCAGAGG CCTCCGCAGC 
TCCAAACGAA ACCTGAGGAA GAGCAGAGAA GACCTGTCTG CTCAGCCGGT CCAAACCAAA 
TTCCCTGCTT ATGAAAGAGT GGTGCTTAGA GAAGCTGGTT TCTTGAGGCC AGTGGTCATT 
TTCGGACCGA TCGCGGATGT GGCTAGAGAG AAGCTGTCCA GAGAGGAGAC TGACCTCTTC 
GAACTTGCAA AGAGTGAACC CAGAGATGCG GGCACAGACC AGCGCAGCTC CGGAATCATC 
CGTCTCCATA CCATTAAACA GATCATTGAC CGAGACAAGC ACGCGGTGCT GGACATCACC 
CCTAATGCGG TGGACCGTCT GAACTACGCT CAGTGGTACC CCATCGTGGT GTTTCTGAAT 
CCAGACAGCA AACAGGGCGT CAAGAACATG AGGACCAGGC TCTGTCCCGA GTCCAGGAAG 
AGCGCCCGAA AGCTCTACGA GCGTGCTCTC AAACTGAGGA AGAACAACCA TCACCTTTTC 
ACCACCACTA TTAACTTGAA CAACATGAAT GACGGGTGGT ACGGTGCTTT GAAAGAGAGC 
ATCCAGCAGC AGCAGAACCA GTTGGTCTGG GTCTCAGAGG GCAAGGCCGA CGGCGCTCCT 
GACGATGACC TTGACATCCA TGACGACCGC CTCTCCTACC TCTCGGCTCC GGGCAGCGAG 
TACAGCATGT ACAGCACCGA CAGCCGCCAC ACGTCCGACT ATGAGGACAC CGACACGGAG 
GGTGGTGCCT ACACCGACCA GGAGCTGGAC GAGACCATGA ACGATGAGGT GGGCCTGCCT 
AGCGAGCCTG CCATCACACG CTCATCTGAG CCTGTGCGAG AGGACCCGCC AGTCATTCAG 
GACGCAGCTG GATACCCCAG CTACCAGCAT GCGGTACCGC AACCTGAAGC GGTGAGCCGC 
ATTGACCCGG CCGGGTTTAA GATGGCAGCG CCGCAGCAGC AAGCTGAGGC TGCTCTGGCC 
TCGCCGCCCC CTCCCTCTGC AGCGGTCCAG CCCACCGCAA CACTAGCGGG TACGAACCCC 
GAGGAGCCGC CTGGCACCCC TCAGGCCGAC TCCCTTAACA GCCCCGTCCC TGTTCCCGAC 
CCCCAGCCCG AACCTGAGCT CGCTCAGCCC CCAACACACG ACCCCCACCA GTCGCTTCCT 
CCTGGCCCAG ATCCAAAGAT GTATAAAAAG GATCTGTACA GCATGGACGA GCCGGTGCGA 
GTGACCCACG GCGTGAAGCC TCCCCCTCCC CAGCAGCAGC AGCAGCCTTT AGGCCCCCCA 
ACCTCGCTCT CCTACAGCCA CCAGCCTGTC TACCAGGACC AACAGCCATA CCGCCAGTAC 
GACCACCCGC CTTATGGCTA TGACGGTGGC GGCTACGCAC AACCAAAGCC TCACAACTAC 
GACCCGCACC TGCACTACGA CAACCGTGTG CCTCACTATA ACGAGCAGTG GCCCACGTAC 
GACCAGCAGC AGCAGCAGCA GACTTCCCCC CAGCCGCCAC CTGCGTCCGG CTTCCCTCAG 
GGCCACCAGC CTCCGCCTCC ACCACCGATG GGATATGAGC CACGCTCCCC ATACGAGGAC 
GGACCTAACA GGGATTTCAG CCCTCCTCAG TCCCAGTATG ACGGTGTGTC CCCAATGGGC 
TACGATCACC GGCCGCGGCA CCCCAAACCT GCACCGGTCC GATATGAAGA GCCTCCACCT 
CCACCTCCTC CGGTGTCCTA CGATGCACGG TCACCTTTCG AACCCGAGCC GCATGCTTTC 
CCAGGCAACA CACATCGCTC GCCAGACCCA CCGAAACAGT ATTATGGAGA CGCCGCTATG 
AGGCCCTTGT ACAACCCTGG ACCTCCGAAC AGAGTGTACA AACATGAGCC GGTGAACTCT 
GAGCCTCCTG TACCGCCTCC CAAACCTGAA TCTGTCCTGT CTCCAGGAGA ACCGCCCCAT 
TCTGCAGCTT CCAAACCATT GCCGCCTCCT CCCAGAGATG ATGACGATGA TGATGACGAT 
GATGATGACC CTGCCATGAA GCCCCAGTCT GTGCTCAACA GGGTCAAAAT GTTCGAAAAC 
AAACGCTCTG TGTCGGTAGA CCGAGCCAAG GACGCACCTG ACGTCACTGG AATTAGGCCC 
ACAGATCTCC CCAAACCTGC GAGCACCCCT GGACCTGTGC TCAAAGCCAA CTCTCTCAGC 
AACCTGGAGC AGGACAAACC CTCCTACAGA GCTCCAGAGC CGCAGAAACC TCAGACGCGT 
GTTGGGGACG ATGTGGTTCG CTCCAACCAC TACGACCCTG ATGATGATGA AGAGTACTAC 
AGAAAACAGC TCTCATTTTT CGACCGCCGC AGTTTCGACA GTAAGGTCAT GAATCCACCC 
AGCGCTGGAG TAAACCGCTT CCATGAGCCG CCCAAACCAC CCCAGACACA GATCGCTTAT 
CCTTTCGCTA GGACGGAGTC TGTGGAGAAG GTGAGTCCTG TGGACAAGAG ATACGAGCCT 
CTGCCGCCAG TCAACCCTTC ACCTGCTCCA TACAGCCAGA ACACACCTGT CGCTCCGCCC 
ACATCCCTGC CCAAACTCAC CAACATCGAG GATCCTCACA GCTCTCCCAA AGCCAAACCG 
GACCTGTCTG CTCTGAGAGC TCCGACCCGG GACGAGCCCA TCCAGAGCAG CTACTTGCCC 
CCCAAGTCCA CCATCAATGG CATGGATGCT CCTCCCAAAA CACTCGGCGT GCCGACCAGC 
TACAACCGCT ACGTCCCCAA GCCTTACACC AGCTCGGCGC GACCCTTCGA GCGCAAGTTC 
GAGAGTCCGA AATTCAACCA CAACCTGCTT CCCAACGACA CGCAGTCCAA ACCCAGTGTC 
AACAACAACC TGAAGCCTCA GATCTCCCCA CAACCCCAGG ACACGGACAG CGGCGTCGAC 
ACCTTCACTC GCACCATGGA CAACAGGCCA AAATATCAGC ACAACAACAT CAACGCCATT 
CCCAAAGCCG TCCCAGTCAG CCCCAGTGCA CTGGATGATG ATGAAGAAGA TGAGGGACAC 
ACAGTTGTGG CCACGGCGCG GGGCATCTTT AACTGTAATG GCGGGGTGTT GAGCTCTATA 
GAGACGGGCG TCAGCATCAT TATCCCACAG GGAGCCATTC CTGATAGTGT GGAGCAGGAG 
ATCTACTTCA AAGTGTGCCG GGACAACAGC ATCCTGCCTC CTCTGGATAA GGAGAAAGGT 
GAAACTCTGC TGAGTCCGCT GGTGATGTGT GGTCCTCACG GCCTGAAGTT CCTGAAGCCT 
GTGGAGCTGC GCTTGCCACA CTGTGCGTCT ATGACCCCTG ATGGTTGGTC TTTTGCTCTA 
AAATCCTCCG ACTCCTCGTC GGGTGACCCC AAATGCTGGC AAAACAAGTG TCTACCAGGA 
GACCCCAATT ACCTGGTGGG CGCAAACTGT GTGTCTGTGC TGATCGACCA CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp1b ( XM_021470727.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326402.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X5, mRNA.

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CloneID	ODa44619
Clone ID Related Accession (Same CDS sequence)	XM_021470727.1
Accession Version	XM_021470727.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6096bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tight junction protein ZO-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
TCCCGCAAGA ATGAAGCATG TATGGCGTCA GGAAGTTCTC AGAAGGTGAC ATCAGCAGAA 
TACGGGCTTC GCCTGGCCAG CCACATCTTC GTGAAGGACA TTTCCCCCGA GAGCCTGGCA 
GCCCGAGACG GAAACATCCA AGAGGGAGAC GTGGTGCTTA AGATTAATGG CACGGTAACA 
GAGAATTTGT CCCTGATCGA TGCTAAGAAG CTGATCGAGA GGTCAAAGGG AAAGCTGAAG 
ATGGTGGTGC AGAGAGACGA GCGGGCGACG CTGCTGAACA TCCCCGATCT GGACGACAGC 
ATACCTTCTG CTAATGCCTC GGACAGAGAT GATATTTCAG ACATCCACTC CGTGGCCTCT 
GATCATTCCA ACCGATCCCA CGACCGGAAG AGGAGCAGCC GCTCTCGTTC TCCAGACCGC 
CGCTCCGAAC CCTCAGACCA CTCCAGACAC TCTCCACCAC AGATCAGCAA CGGCAGTCAC 
AGGAGCCGTG ATGAAGACAG GATCTCCAAG GCCGGGCCGC TTCCTCTACC TGCTAAGATG 
GTGGAGGAGA TGCCGAAAGA GCAGAGCGCA GCCAGAGAAA CAGAGAAACA GCTCCCCCCT 
CTGCCAGAGC CCAAACCTGT GTACGCTCAG CCGGGCCAGC CGGACGTGGA CCTGCCTGTT 
AGTCCGGCAG ATGCTCCTGT GCCCAGCGTC ACACATGATG ACAGCATCTT ACGGCCGAGC 
ATGAAGCTGG TGAAGTTCAA GAAGGGCGAG AGCGTGGGCT TGAGGCTCGC TGGGGGGAAC 
GACGTTGGGA TATTTGTGGC GGGAGTTCTG GAGGACAGTC CTGCTGCTAA AGAGGGCCTG 
GAAGAGGGAG ACCAGATTCT CAGGGTAAAT AATGTGGATT TTGCAAATAT CATCCGAGAG 
GAGGCAGTGC TGTTCCTGCT GGATCTGCCC AAAGGAGAGG AGGTCACCAT CCTGGCCCAG 
AAGAAGAAAG ACGTGTACCG CCGCATTGTA GAGTCTGACG TGGGAGACTC GTTCTACATC 
AGGACTCATT TTGAATATGA GAAGGAGTCG CCCTATGGCC TGAGCTTCAA TAAGGGGGAG 
GTCTTCAGAG TCGTCGACAC CCTTTACAAT GGCAAACTGG GCTCCTGGCT CGCCATCCGC 
ATCGGCAAAA ACCACCAGGA GGTGGAGCGG GGCATCATCC CCAACAAGAA CAGGGCTGAG 
CAGTTATCAA GTGTCCAGTA CACTCTCCCA AAGACTCCAG GAGGGGATCG CGCCGACTTC 
TGGAGATTCA GAGGCCTCCG CAGCTCCAAA CGAAACCTGA GGAAGAGCAG AGAAGACCTG 
TCTGCTCAGC CGGTCCAAAC CAAATTCCCT GCTTATGAAA GAGTGGTGCT TAGAGAAGCT 
GGTTTCTTGA GGCCAGTGGT CATTTTCGGA CCGATCGCGG ATGTGGCTAG AGAGAAGCTG 
TCCAGAGAGG AGACTGACCT CTTCGAACTT GCAAAGAGTG AACCCAGAGA TGCGGGCACA 
GACCAGCGCA GCTCCGGAAT CATCCGTCTC CATACCATTA AACAGATCAT TGACCGAGAC 
AAGCACGCGG TGCTGGACAT CACCCCTAAT GCGGTGGACC GTCTGAACTA CGCTCAGTGG 
TACCCCATCG TGGTGTTTCT GAATCCAGAC AGCAAACAGG GCGTCAAGAA CATGAGGACC 
AGGCTCTGTC CCGAGTCCAG GAAGAGCGCC CGAAAGCTCT ACGAGCGTGC TCTCAAACTG 
AGGAAGAACA ACCATCACCT TTTCACCACC ACTATTAACT TGAACAACAT GAATGACGGG 
TGGTACGGTG CTTTGAAAGA GAGCATCCAG CAGCAGCAGA ACCAGTTGGT CTGGGTCTCA 
GAGGGCAAGG CCGACGGCGC TCCTGACGAT GACCTTGACA TCCATGACGA CCGCCTCTCC 
TACCTCTCGG CTCCGGGCAG CGAGTACAGC ATGTACAGCA CCGACAGCCG CCACACGTCC 
GACTATGAGG ACACCGACAC GGAGGGTGGT GCCTACACCG ACCAGGAGCT GGACGAGACC 
ATGAACGATG AGGTGGGCCT GCCTAGCGAG CCTGCCATCA CACGCTCATC TGAGCCTGTG 
CGAGAGGACC CGCCAGTCAT TCAGGACGCA GCTGGATACC CCAGCTACCA GCATGCGGTA 
CCGCAACCTG AAGCGGTGAG CCGCATTGAC CCGGCCGGGT TTAAGATGGC AGCGCCGCAG 
CAGCAAGCTG AGGCTGCTCT GGCCTCGCCG CCCCCTCCCT CTGCAGCGGT CCAGCCCACC 
GCAACACTAG CGGGTACGAA CCCCGAGGAG CCGCCTGGCA CCCCTCAGGC CGACTCCCTT 
AACAGCCCCG TCCCTGTTCC CGACCCCCAG CCCGAACCTG AGCTCGCTCA GCCCCCAACA 
CACGACCCCC ACCAGTCGCT TCCTCCTGGC CCAGATCCAA AGATGTATAA AAAGGATCTG 
TACAGCATGG ACGAGCCGGT GCGAGTGACC CACGGCGTGA AGCCTCCCCC TCCCCAGCAG 
CAGCAGCAGC CTTTAGGCCC CCCAACCTCG CTCTCCTACA GCCACCAGCC TGTCTACCAG 
GACCAACAGC CATACCGCCA GTACGACCAC CCGCCTTATG GCTATGACGG TGGCGGCTAC 
GCACAACCAA AGCCTCACAA CTACGACCCG CACCTGCACT ACGACAACCG TGTGCCTCAC 
TATAACGAGC AGTGGCCCAC GTACGACCAG CAGCAGCAGC AGCAGACTTC CCCCCAGCCG 
CCACCTGCGT CCGGCTTCCC TCAGGGCCAC CAGCCTCCGC CTCCACCACC GATGGGATAT 
GAGCCACGCT CCCCATACGA GGACGGACCT AACAGGGATT TCAGCCCTCC TCAGTCCCAG 
TATGACGGTG TGTCCCCAAT GGGCTACGAT CACCGGCCGC GGCACCCCAA ACCTGCACCG 
GTCCGATATG AAGAGCCTCC ACCTCCACCT CCTCCGGTGT CCTACGATGC ACGGTCACCT 
TTCGAACCCG AGCCGCATGC TTTCCCAGGC AACACACATC GCTCGCCAGA CCCACCGAAA 
CAGTATTATG GAGACGCCGC TATGAGGCCC TTGTACAACC CTGGACCTCC GAACAGAGTG 
TACAAACATG AGCCGGTGAA CTCTGAGCCT CCTGTACCGC CTCCCAAACC TGAATCTGTC 
CTGTCTCCAG GAGAACCGCC CCATTCTGCA GCTTCCAAAC CATTGCCGCC TCCTCCCAGA 
GATGATGACG ATGATGATGA CGATGATGAT GACCCTGCCA TGAAGCCCCA GTCTGTGCTC 
AACAGGGTCA AAATGTTCGA AAACAAACGC TCTGTGTCGG TAGACCGAGC CAAGGACGCA 
CCTGACGTCA CTGGAATTAG GCCCACAGAT CTCCCCAAAC CTGCGAGCAC CCCTGGACCT 
GTGCTCAAAG CCAACTCTCT CAGCAACCTG GAGCAGGACA AACCCTCCTA CAGAGCTCCA 
GAGCCGCAGA AACCTCAGAC GCGTGTTGGG GACGATGTGG TTCGCTCCAA CCACTACGAC 
CCTGATGATG ATGAAGAGTA CTACAGAAAA CAGCTCTCAT TTTTCGACCG CCGCAGTTTC 
GACAGTAAGG TCATGAATCC ACCCAGCGCT GGAGTAAACC GCTTCCATGA GCCGCCCAAA 
CCACCCCAGA CACAGATCGC TTATCCTTTC GCTAGGACGG AGTCTGTGGA GAAGGTGAGT 
CCTGTGGACA AGAGATACGA GCCTCTGCCG CCAGTCAACC CTTCACCTGC TCCATACAGC 
CAGAACACAC CTGTCGCTCC GCCCACATCC CTGCCCAAAC TCACCAACAT CGAGGTGAAC 
TCTCTGCCAG ATCCTCACAG CTCTCCCAAA GCCAAACCGG ACCTGTCTGC TCTGAGAGCT 
CCGACCCGGG ACGAGCCCAT CCAGAGCAGC TACTTGCCCC CCAAGTCCAC CATCAATGGC 
ATGGATGCTC CTCCCAAAAC ACTCGGCGTG CCGACCAGCT ACAACCGCTA CGTCCCCAAG 
CCTTACACCA GCTCGGCGCG ACCCTTCGAG CGCAAGTTCG AGAGTCCGAA ATTCAACCAC 
AACCTGCTTC CCAACGACAC GCAGTCCAAA CCCAGTGTCA ACAACAACCT GAAGCCTCAG 
ATCTCCCCAC AACCCCAGGA CACGGACAGC GGCGTCGACA CCTTCACTCG CACCATGGAC 
AACAGGCCAA AATATCAGCA CAACAACATC AACGCCATTC CCAAAGCCGT CCCAGTCAGC 
CCCAGTGCAC TGGATGATGA TGAAGAAGAT GAGGGACACA CAGTTGTGGC CACGGCGCGG 
GGCATCTTTA ACTGTAATGG CGGGGTGTTG AGCTCTATAG AGACGGGCGT CAGCATCATT 
ATCCCACAGG GAGCCATTCC TGATAGTGTG GAGCAGGAGA TCTACTTCAA AGTGTGCCGG 
GACAACAGCA TCCTGCCTCC TCTGGATAAG GAGAAAGGTG AAACTCTGCT GAGTCCGCTG 
GTGATGTGTG GTCCTCACGG CCTGAAGTTC CTGAAGCCTG TGGAGCTGCG CTTGCCACAC 
TGTGCGTCTA TGACCCCTGA TGGTTGGTCT TTTGCTCTAA AATCCTCCGA CTCCTCGTCG 
GGTGACCCCA AATGCTGGCA AAACAAGTGT CTACCAGGAG ACCCCAATTA CCTGGTGGGC 
GCAAACTGTG TGTCTGTGCT GATCGACCAC TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326402.1
CDS	110..6205
Translation	MLLSVFGGPVEEVLAGLLKLKMFGSGSKKWFIKKNKRQRTKVQRKPASGRRNKRVSRVLKRHKEVIVKQLDVSKVLPTLVYERVFTLGEYKEILGQDSSGKRAELFLDLLSQKEPAAFSAFCGVLEEVCPHLLTCFLLDGTDGSAGENKKQGQTKPAHSQQNALSKGQDTVCFPPMYTDPLFDTRVFPKHDKDKYGPAVYYPKFNAYIPNPDKVSVLDESEGSGHGKPSLRRIKGRIHRSKSLDSIDLLDSNSAAMDETVIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGLLQENDRVVMVNAVSMDNVDHAYAVQQLRKSGKNAKITIRRKRKVQIPSGRPGERETMSEHDEDDDSYEDEIYESRSARSGPSAYSGGGGGGGGGGASGRRSGRGDRLSGRRERDRERSGSRERSLSPRSDRHSVSSNLPPRPAKVTLVKSRKNEACMASGSSQKVTSAEYGLRLASHIFVKDISPESLAARDGNIQEGDVVLKINGTVTENLSLIDAKKLIERSKGKLKMVVQRDERATLLNIPDLDDSIPSANASDRDDISDIHSVASDHSNRSHDRKRSSRSRSPDRRSEPSDHSRHSPPQISNGSHRSRDEDRISKAGPLPLPAKMVEEMPKEQSAARETEKQLPPLPEPKPVYAQPGQPDVDLPVSPADAPVPSVTHDDSILRPSMKLVKFKKGESVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFANIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHQEVERGIIPNKNRAEQLSSVQYTLPKTPGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVVIFGPIADVAREKLSREETDLFELAKSEPRDAGTDQRSSGIIRLHTIKQIIDRDKHAVLDITPNAVDRLNYAQWYPIVVFLNPDSKQGVKNMRTRLCPESRKSARKLYERALKLRKNNHHLFTTTINLNNMNDGWYGALKESIQQQQNQLVWVSEGKADGAPDDDLDIHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETMNDEVGLPSEPAITRSSEPVREDPPVIQDAAGYPSYQHAVPQPEAVSRIDPAGFKMAAPQQQAEAALASPPPPSAAVQPTATLAGTNPEEPPGTPQADSLNSPVPVPDPQPEPELAQPPTHDPHQSLPPGPDPKMYKKDLYSMDEPVRVTHGVKPPPPQQQQQPLGPPTSLSYSHQPVYQDQQPYRQYDHPPYGYDGGGYAQPKPHNYDPHLHYDNRVPHYNEQWPTYDQQQQQQTSPQPPPASGFPQGHQPPPPPPMGYEPRSPYEDGPNRDFSPPQSQYDGVSPMGYDHRPRHPKPAPVRYEEPPPPPPPVSYDARSPFEPEPHAFPGNTHRSPDPPKQYYGDAAMRPLYNPGPPNRVYKHEPVNSEPPVPPPKPESVLSPGEPPHSAASKPLPPPPRDDDDDDDDDDDPAMKPQSVLNRVKMFENKRSVSVDRAKDAPDVTGIRPTDLPKPASTPGPVLKANSLSNLEQDKPSYRAPEPQKPQTRVGDDVVRSNHYDPDDDEEYYRKQLSFFDRRSFDSKVMNPPSAGVNRFHEPPKPPQTQIAYPFARTESVEKVSPVDKRYEPLPPVNPSPAPYSQNTPVAPPTSLPKLTNIEVNSLPDPHSSPKAKPDLSALRAPTRDEPIQSSYLPPKSTINGMDAPPKTLGVPTSYNRYVPKPYTSSARPFERKFESPKFNHNLLPNDTQSKPSVNNNLKPQISPQPQDTDSGVDTFTRTMDNRPKYQHNNINAIPKAVPVSPSALDDDEEDEGHTVVATARGIFNCNGGVLSSIETGVSIIIPQGAIPDSVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKCWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_021470727.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tight junction protein 1b (tjp1b), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470727.1

ORF Insert Sequence:

ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
TCCCGCAAGA ATGAAGCATG TATGGCGTCA GGAAGTTCTC AGAAGGTGAC ATCAGCAGAA 
TACGGGCTTC GCCTGGCCAG CCACATCTTC GTGAAGGACA TTTCCCCCGA GAGCCTGGCA 
GCCCGAGACG GAAACATCCA AGAGGGAGAC GTGGTGCTTA AGATTAATGG CACGGTAACA 
GAGAATTTGT CCCTGATCGA TGCTAAGAAG CTGATCGAGA GGTCAAAGGG AAAGCTGAAG 
ATGGTGGTGC AGAGAGACGA GCGGGCGACG CTGCTGAACA TCCCCGATCT GGACGACAGC 
ATACCTTCTG CTAATGCCTC GGACAGAGAT GATATTTCAG ACATCCACTC CGTGGCCTCT 
GATCATTCCA ACCGATCCCA CGACCGGAAG AGGAGCAGCC GCTCTCGTTC TCCAGACCGC 
CGCTCCGAAC CCTCAGACCA CTCCAGACAC TCTCCACCAC AGATCAGCAA CGGCAGTCAC 
AGGAGCCGTG ATGAAGACAG GATCTCCAAG GCCGGGCCGC TTCCTCTACC TGCTAAGATG 
GTGGAGGAGA TGCCGAAAGA GCAGAGCGCA GCCAGAGAAA CAGAGAAACA GCTCCCCCCT 
CTGCCAGAGC CCAAACCTGT GTACGCTCAG CCGGGCCAGC CGGACGTGGA CCTGCCTGTT 
AGTCCGGCAG ATGCTCCTGT GCCCAGCGTC ACACATGATG ACAGCATCTT ACGGCCGAGC 
ATGAAGCTGG TGAAGTTCAA GAAGGGCGAG AGCGTGGGCT TGAGGCTCGC TGGGGGGAAC 
GACGTTGGGA TATTTGTGGC GGGAGTTCTG GAGGACAGTC CTGCTGCTAA AGAGGGCCTG 
GAAGAGGGAG ACCAGATTCT CAGGGTAAAT AATGTGGATT TTGCAAATAT CATCCGAGAG 
GAGGCAGTGC TGTTCCTGCT GGATCTGCCC AAAGGAGAGG AGGTCACCAT CCTGGCCCAG 
AAGAAGAAAG ACGTGTACCG CCGCATTGTA GAGTCTGACG TGGGAGACTC GTTCTACATC 
AGGACTCATT TTGAATATGA GAAGGAGTCG CCCTATGGCC TGAGCTTCAA TAAGGGGGAG 
GTCTTCAGAG TCGTCGACAC CCTTTACAAT GGCAAACTGG GCTCCTGGCT CGCCATCCGC 
ATCGGCAAAA ACCACCAGGA GGTGGAGCGG GGCATCATCC CCAACAAGAA CAGGGCTGAG 
CAGTTATCAA GTGTCCAGTA CACTCTCCCA AAGACTCCAG GAGGGGATCG CGCCGACTTC 
TGGAGATTCA GAGGCCTCCG CAGCTCCAAA CGAAACCTGA GGAAGAGCAG AGAAGACCTG 
TCTGCTCAGC CGGTCCAAAC CAAATTCCCT GCTTATGAAA GAGTGGTGCT TAGAGAAGCT 
GGTTTCTTGA GGCCAGTGGT CATTTTCGGA CCGATCGCGG ATGTGGCTAG AGAGAAGCTG 
TCCAGAGAGG AGACTGACCT CTTCGAACTT GCAAAGAGTG AACCCAGAGA TGCGGGCACA 
GACCAGCGCA GCTCCGGAAT CATCCGTCTC CATACCATTA AACAGATCAT TGACCGAGAC 
AAGCACGCGG TGCTGGACAT CACCCCTAAT GCGGTGGACC GTCTGAACTA CGCTCAGTGG 
TACCCCATCG TGGTGTTTCT GAATCCAGAC AGCAAACAGG GCGTCAAGAA CATGAGGACC 
AGGCTCTGTC CCGAGTCCAG GAAGAGCGCC CGAAAGCTCT ACGAGCGTGC TCTCAAACTG 
AGGAAGAACA ACCATCACCT TTTCACCACC ACTATTAACT TGAACAACAT GAATGACGGG 
TGGTACGGTG CTTTGAAAGA GAGCATCCAG CAGCAGCAGA ACCAGTTGGT CTGGGTCTCA 
GAGGGCAAGG CCGACGGCGC TCCTGACGAT GACCTTGACA TCCATGACGA CCGCCTCTCC 
TACCTCTCGG CTCCGGGCAG CGAGTACAGC ATGTACAGCA CCGACAGCCG CCACACGTCC 
GACTATGAGG ACACCGACAC GGAGGGTGGT GCCTACACCG ACCAGGAGCT GGACGAGACC 
ATGAACGATG AGGTGGGCCT GCCTAGCGAG CCTGCCATCA CACGCTCATC TGAGCCTGTG 
CGAGAGGACC CGCCAGTCAT TCAGGACGCA GCTGGATACC CCAGCTACCA GCATGCGGTA 
CCGCAACCTG AAGCGGTGAG CCGCATTGAC CCGGCCGGGT TTAAGATGGC AGCGCCGCAG 
CAGCAAGCTG AGGCTGCTCT GGCCTCGCCG CCCCCTCCCT CTGCAGCGGT CCAGCCCACC 
GCAACACTAG CGGGTACGAA CCCCGAGGAG CCGCCTGGCA CCCCTCAGGC CGACTCCCTT 
AACAGCCCCG TCCCTGTTCC CGACCCCCAG CCCGAACCTG AGCTCGCTCA GCCCCCAACA 
CACGACCCCC ACCAGTCGCT TCCTCCTGGC CCAGATCCAA AGATGTATAA AAAGGATCTG 
TACAGCATGG ACGAGCCGGT GCGAGTGACC CACGGCGTGA AGCCTCCCCC TCCCCAGCAG 
CAGCAGCAGC CTTTAGGCCC CCCAACCTCG CTCTCCTACA GCCACCAGCC TGTCTACCAG 
GACCAACAGC CATACCGCCA GTACGACCAC CCGCCTTATG GCTATGACGG TGGCGGCTAC 
GCACAACCAA AGCCTCACAA CTACGACCCG CACCTGCACT ACGACAACCG TGTGCCTCAC 
TATAACGAGC AGTGGCCCAC GTACGACCAG CAGCAGCAGC AGCAGACTTC CCCCCAGCCG 
CCACCTGCGT CCGGCTTCCC TCAGGGCCAC CAGCCTCCGC CTCCACCACC GATGGGATAT 
GAGCCACGCT CCCCATACGA GGACGGACCT AACAGGGATT TCAGCCCTCC TCAGTCCCAG 
TATGACGGTG TGTCCCCAAT GGGCTACGAT CACCGGCCGC GGCACCCCAA ACCTGCACCG 
GTCCGATATG AAGAGCCTCC ACCTCCACCT CCTCCGGTGT CCTACGATGC ACGGTCACCT 
TTCGAACCCG AGCCGCATGC TTTCCCAGGC AACACACATC GCTCGCCAGA CCCACCGAAA 
CAGTATTATG GAGACGCCGC TATGAGGCCC TTGTACAACC CTGGACCTCC GAACAGAGTG 
TACAAACATG AGCCGGTGAA CTCTGAGCCT CCTGTACCGC CTCCCAAACC TGAATCTGTC 
CTGTCTCCAG GAGAACCGCC CCATTCTGCA GCTTCCAAAC CATTGCCGCC TCCTCCCAGA 
GATGATGACG ATGATGATGA CGATGATGAT GACCCTGCCA TGAAGCCCCA GTCTGTGCTC 
AACAGGGTCA AAATGTTCGA AAACAAACGC TCTGTGTCGG TAGACCGAGC CAAGGACGCA 
CCTGACGTCA CTGGAATTAG GCCCACAGAT CTCCCCAAAC CTGCGAGCAC CCCTGGACCT 
GTGCTCAAAG CCAACTCTCT CAGCAACCTG GAGCAGGACA AACCCTCCTA CAGAGCTCCA 
GAGCCGCAGA AACCTCAGAC GCGTGTTGGG GACGATGTGG TTCGCTCCAA CCACTACGAC 
CCTGATGATG ATGAAGAGTA CTACAGAAAA CAGCTCTCAT TTTTCGACCG CCGCAGTTTC 
GACAGTAAGG TCATGAATCC ACCCAGCGCT GGAGTAAACC GCTTCCATGA GCCGCCCAAA 
CCACCCCAGA CACAGATCGC TTATCCTTTC GCTAGGACGG AGTCTGTGGA GAAGGTGAGT 
CCTGTGGACA AGAGATACGA GCCTCTGCCG CCAGTCAACC CTTCACCTGC TCCATACAGC 
CAGAACACAC CTGTCGCTCC GCCCACATCC CTGCCCAAAC TCACCAACAT CGAGGTGAAC 
TCTCTGCCAG ATCCTCACAG CTCTCCCAAA GCCAAACCGG ACCTGTCTGC TCTGAGAGCT 
CCGACCCGGG ACGAGCCCAT CCAGAGCAGC TACTTGCCCC CCAAGTCCAC CATCAATGGC 
ATGGATGCTC CTCCCAAAAC ACTCGGCGTG CCGACCAGCT ACAACCGCTA CGTCCCCAAG 
CCTTACACCA GCTCGGCGCG ACCCTTCGAG CGCAAGTTCG AGAGTCCGAA ATTCAACCAC 
AACCTGCTTC CCAACGACAC GCAGTCCAAA CCCAGTGTCA ACAACAACCT GAAGCCTCAG 
ATCTCCCCAC AACCCCAGGA CACGGACAGC GGCGTCGACA CCTTCACTCG CACCATGGAC 
AACAGGCCAA AATATCAGCA CAACAACATC AACGCCATTC CCAAAGCCGT CCCAGTCAGC 
CCCAGTGCAC TGGATGATGA TGAAGAAGAT GAGGGACACA CAGTTGTGGC CACGGCGCGG 
GGCATCTTTA ACTGTAATGG CGGGGTGTTG AGCTCTATAG AGACGGGCGT CAGCATCATT 
ATCCCACAGG GAGCCATTCC TGATAGTGTG GAGCAGGAGA TCTACTTCAA AGTGTGCCGG 
GACAACAGCA TCCTGCCTCC TCTGGATAAG GAGAAAGGTG AAACTCTGCT GAGTCCGCTG 
GTGATGTGTG GTCCTCACGG CCTGAAGTTC CTGAAGCCTG TGGAGCTGCG CTTGCCACAC 
TGTGCGTCTA TGACCCCTGA TGGTTGGTCT TTTGCTCTAA AATCCTCCGA CTCCTCGTCG 
GGTGACCCCA AATGCTGGCA AAACAAGTGT CTACCAGGAG ACCCCAATTA CCTGGTGGGC 
GCAAACTGTG TGTCTGTGCT GATCGACCAC TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp1b ( XM_009298130.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296405.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X1, mRNA.

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CloneID	ODa44620
Clone ID Related Accession (Same CDS sequence)	XM_009298130.2 , XM_009298130.3
Accession Version	XM_009298130.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6093bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tight junction protein ZO-1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298130.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
TCCCGCAAGA ATGAAGCAGA ATACGGGCTT CGCCTGGCCA GCCACATCTT CGTGAAGGAC 
ATTTCCCCCG AGAGCCTGGC AGCCCGAGAC GGAAACATCC AAGAGGGAGA CGTGGTGCTT 
AAGATTAATG GCACGGTAAC AGAGAATTTG TCCCTGATCG ATGCTAAGAA GCTGATCGAG 
AGGTCAAAGG GAAAGCTGAA GATGGTGGTG CAGAGAGACG AGCGGGCGAC GCTGCTGAAC 
ATCCCCGATC TGGACGACAG CATACCTTCT GCTAATGCCT CGGACAGAGA TGATATTTCA 
GACATCCACT CCGTGGCCTC TGATCATTCC AACCGATCCC ACGACCGGAA GAGGAGCAGC 
CGCTCTCGTT CTCCAGACCG CCGCTCCGAA CCCTCAGACC ACTCCAGACA CTCTCCACCA 
CAGATCAGCA ACGGCAGGGC CACAGGGCCG ACGGCTCAGA GAACGCGGCC AGTTCACAGG 
AGCCGTGATG AAGACAGGAT CTCCAAGGCC GGGCCGCTTC CTCTACCTGC TAAGATGGTG 
GAGGAGATGC CGAAAGAGCA GAGCGCAGCC AGAGAAACAG AGAAACAGCT CCCCCCTCTG 
CCAGAGCCCA AACCTGTGTA CGCTCAGCCG GGCCAGCCGG ACGTGGACCT GCCTGTTAGT 
CCGGCAGATG CTCCTGTGCC CAGCGTCACA CATGATGACA GCATCTTACG GCCGAGCATG 
AAGCTGGTGA AGTTCAAGAA GGGCGAGAGC GTGGGCTTGA GGCTCGCTGG GGGGAACGAC 
GTTGGGATAT TTGTGGCGGG AGTTCTGGAG GACAGTCCTG CTGCTAAAGA GGGCCTGGAA 
GAGGGAGACC AGATTCTCAG GGTAAATAAT GTGGATTTTG CAAATATCAT CCGAGAGGAG 
GCAGTGCTGT TCCTGCTGGA TCTGCCCAAA GGAGAGGAGG TCACCATCCT GGCCCAGAAG 
AAGAAAGACG TGTACCGCCG CATTGTAGAG TCTGACGTGG GAGACTCGTT CTACATCAGG 
ACTCATTTTG AATATGAGAA GGAGTCGCCC TATGGCCTGA GCTTCAATAA GGGGGAGGTC 
TTCAGAGTCG TCGACACCCT TTACAATGGC AAACTGGGCT CCTGGCTCGC CATCCGCATC 
GGCAAAAACC ACCAGGAGGT GGAGCGGGGC ATCATCCCCA ACAAGAACAG GGCTGAGCAG 
TTATCAAGTG TCCAGTACAC TCTCCCAAAG ACTCCAGGAG GGGATCGCGC CGACTTCTGG 
AGATTCAGAG GCCTCCGCAG CTCCAAACGA AACCTGAGGA AGAGCAGAGA AGACCTGTCT 
GCTCAGCCGG TCCAAACCAA ATTCCCTGCT TATGAAAGAG TGGTGCTTAG AGAAGCTGGT 
TTCTTGAGGC CAGTGGTCAT TTTCGGACCG ATCGCGGATG TGGCTAGAGA GAAGCTGTCC 
AGAGAGGAGA CTGACCTCTT CGAACTTGCA AAGAGTGAAC CCAGAGATGC GGGCACAGAC 
CAGCGCAGCT CCGGAATCAT CCGTCTCCAT ACCATTAAAC AGATCATTGA CCGAGACAAG 
CACGCGGTGC TGGACATCAC CCCTAATGCG GTGGACCGTC TGAACTACGC TCAGTGGTAC 
CCCATCGTGG TGTTTCTGAA TCCAGACAGC AAACAGGGCG TCAAGAACAT GAGGACCAGG 
CTCTGTCCCG AGTCCAGGAA GAGCGCCCGA AAGCTCTACG AGCGTGCTCT CAAACTGAGG 
AAGAACAACC ATCACCTTTT CACCACCACT ATTAACTTGA ACAACATGAA TGACGGGTGG 
TACGGTGCTT TGAAAGAGAG CATCCAGCAG CAGCAGAACC AGTTGGTCTG GGTCTCAGAG 
GGCAAGGCCG ACGGCGCTCC TGACGATGAC CTTGACATCC ATGACGACCG CCTCTCCTAC 
CTCTCGGCTC CGGGCAGCGA GTACAGCATG TACAGCACCG ACAGCCGCCA CACGTCCGAC 
TATGAGGACA CCGACACGGA GGGTGGTGCC TACACCGACC AGGAGCTGGA CGAGACCATG 
AACGATGAGG TGGGCCTGCC TAGCGAGCCT GCCATCACAC GCTCATCTGA GCCTGTGCGA 
GAGGACCCGC CAGTCATTCA GGACGCAGCT GGATACCCCA GCTACCAGCA TGCGGTACCG 
CAACCTGAAG CGGTGAGCCG CATTGACCCG GCCGGGTTTA AGATGGCAGC GCCGCAGCAG 
CAAGCTGAGG CTGCTCTGGC CTCGCCGCCC CCTCCCTCTG CAGCGGTCCA GCCCACCGCA 
ACACTAGCGG GTACGAACCC CGAGGAGCCG CCTGGCACCC CTCAGGCCGA CTCCCTTAAC 
AGCCCCGTCC CTGTTCCCGA CCCCCAGCCC GAACCTGAGC TCGCTCAGCC CCCAACACAC 
GACCCCCACC AGTCGCTTCC TCCTGGCCCA GATCCAAAGA TGTATAAAAA GGATCTGTAC 
AGCATGGACG AGCCGGTGCG AGTGACCCAC GGCGTGAAGC CTCCCCCTCC CCAGCAGCAG 
CAGCAGCCTT TAGGCCCCCC AACCTCGCTC TCCTACAGCC ACCAGCCTGT CTACCAGGAC 
CAACAGCCAT ACCGCCAGTA CGACCACCCG CCTTATGGCT ATGACGGTGG CGGCTACGCA 
CAACCAAAGC CTCACAACTA CGACCCGCAC CTGCACTACG ACAACCGTGT GCCTCACTAT 
AACGAGCAGT GGCCCACGTA CGACCAGCAG CAGCAGCAGC AGACTTCCCC CCAGCCGCCA 
CCTGCGTCCG GCTTCCCTCA GGGCCACCAG CCTCCGCCTC CACCACCGAT GGGATATGAG 
CCACGCTCCC CATACGAGGA CGGACCTAAC AGGGATTTCA GCCCTCCTCA GTCCCAGTAT 
GACGGTGTGT CCCCAATGGG CTACGATCAC CGGCCGCGGC ACCCCAAACC TGCACCGGTC 
CGATATGAAG AGCCTCCACC TCCACCTCCT CCGGTGTCCT ACGATGCACG GTCACCTTTC 
GAACCCGAGC CGCATGCTTT CCCAGGCAAC ACACATCGCT CGCCAGACCC ACCGAAACAG 
TATTATGGAG ACGCCGCTAT GAGGCCCTTG TACAACCCTG GACCTCCGAA CAGAGTGTAC 
AAACATGAGC CGGTGAACTC TGAGCCTCCT GTACCGCCTC CCAAACCTGA ATCTGTCCTG 
TCTCCAGGAG AACCGCCCCA TTCTGCAGCT TCCAAACCAT TGCCGCCTCC TCCCAGAGAT 
GATGACGATG ATGATGACGA TGATGATGAC CCTGCCATGA AGCCCCAGTC TGTGCTCAAC 
AGGGTCAAAA TGTTCGAAAA CAAACGCTCT GTGTCGGTAG ACCGAGCCAA GGACGCACCT 
GACGTCACTG GAATTAGGCC CACAGATCTC CCCAAACCTG CGAGCACCCC TGGACCTGTG 
CTCAAAGCCA ACTCTCTCAG CAACCTGGAG CAGGACAAAC CCTCCTACAG AGCTCCAGAG 
CCGCAGAAAC CTCAGACGCG TGTTGGGGAC GATGTGGTTC GCTCCAACCA CTACGACCCT 
GATGATGATG AAGAGTACTA CAGAAAACAG CTCTCATTTT TCGACCGCCG CAGTTTCGAC 
AGTAAGGTCA TGAATCCACC CAGCGCTGGA GTAAACCGCT TCCATGAGCC GCCCAAACCA 
CCCCAGACAC AGATCGCTTA TCCTTTCGCT AGGACGGAGT CTGTGGAGAA GGTGAGTCCT 
GTGGACAAGA GATACGAGCC TCTGCCGCCA GTCAACCCTT CACCTGCTCC ATACAGCCAG 
AACACACCTG TCGCTCCGCC CACATCCCTG CCCAAACTCA CCAACATCGA GGTGAACTCT 
CTGCCAGATC CTCACAGCTC TCCCAAAGCC AAACCGGACC TGTCTGCTCT GAGAGCTCCG 
ACCCGGGACG AGCCCATCCA GAGCAGCTAC TTGCCCCCCA AGTCCACCAT CAATGGCATG 
GATGCTCCTC CCAAAACACT CGGCGTGCCG ACCAGCTACA ACCGCTACGT CCCCAAGCCT 
TACACCAGCT CGGCGCGACC CTTCGAGCGC AAGTTCGAGA GTCCGAAATT CAACCACAAC 
CTGCTTCCCA ACGACACGCA GTCCAAACCC AGTGTCAACA ACAACCTGAA GCCTCAGATC 
TCCCCACAAC CCCAGGACAC GGACAGCGGC GTCGACACCT TCACTCGCAC CATGGACAAC 
AGGCCAAAAT ATCAGCACAA CAACATCAAC GCCATTCCCA AAGCCGTCCC AGTCAGCCCC 
AGTGCACTGG ATGATGATGA AGAAGATGAG GGACACACAG TTGTGGCCAC GGCGCGGGGC 
ATCTTTAACT GTAATGGCGG GGTGTTGAGC TCTATAGAGA CGGGCGTCAG CATCATTATC 
CCACAGGGAG CCATTCCTGA TAGTGTGGAG CAGGAGATCT ACTTCAAAGT GTGCCGGGAC 
AACAGCATCC TGCCTCCTCT GGATAAGGAG AAAGGTGAAA CTCTGCTGAG TCCGCTGGTG 
ATGTGTGGTC CTCACGGCCT GAAGTTCCTG AAGCCTGTGG AGCTGCGCTT GCCACACTGT 
GCGTCTATGA CCCCTGATGG TTGGTCTTTT GCTCTAAAAT CCTCCGACTC CTCGTCGGGT 
GACCCCAAAT GCTGGCAAAA CAAGTGTCTA CCAGGAGACC CCAATTACCT GGTGGGCGCA 
AACTGTGTGT CTGTGCTGAT CGACCACTTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296405.1
CDS	115..6207
Translation	MLLSVFGGPVEEVLAGLLKLKMFGSGSKKWFIKKNKRQRTKVQRKPASGRRNKRVSRVLKRHKEVIVKQLDVSKVLPTLVYERVFTLGEYKEILGQDSSGKRAELFLDLLSQKEPAAFSAFCGVLEEVCPHLLTCFLLDGTDGSAGENKKQGQTKPAHSQQNALSKGQDTVCFPPMYTDPLFDTRVFPKHDKDKYGPAVYYPKFNAYIPNPDKVSVLDESEGSGHGKPSLRRIKGRIHRSKSLDSIDLLDSNSAAMDETVIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGLLQENDRVVMVNAVSMDNVDHAYAVQQLRKSGKNAKITIRRKRKVQIPSGRPGERETMSEHDEDDDSYEDEIYESRSARSGPSAYSGGGGGGGGGGASGRRSGRGDRLSGRRERDRERSGSRERSLSPRSDRHSVSSNLPPRPAKVTLVKSRKNEAEYGLRLASHIFVKDISPESLAARDGNIQEGDVVLKINGTVTENLSLIDAKKLIERSKGKLKMVVQRDERATLLNIPDLDDSIPSANASDRDDISDIHSVASDHSNRSHDRKRSSRSRSPDRRSEPSDHSRHSPPQISNGRATGPTAQRTRPVHRSRDEDRISKAGPLPLPAKMVEEMPKEQSAARETEKQLPPLPEPKPVYAQPGQPDVDLPVSPADAPVPSVTHDDSILRPSMKLVKFKKGESVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFANIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHQEVERGIIPNKNRAEQLSSVQYTLPKTPGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVVIFGPIADVAREKLSREETDLFELAKSEPRDAGTDQRSSGIIRLHTIKQIIDRDKHAVLDITPNAVDRLNYAQWYPIVVFLNPDSKQGVKNMRTRLCPESRKSARKLYERALKLRKNNHHLFTTTINLNNMNDGWYGALKESIQQQQNQLVWVSEGKADGAPDDDLDIHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETMNDEVGLPSEPAITRSSEPVREDPPVIQDAAGYPSYQHAVPQPEAVSRIDPAGFKMAAPQQQAEAALASPPPPSAAVQPTATLAGTNPEEPPGTPQADSLNSPVPVPDPQPEPELAQPPTHDPHQSLPPGPDPKMYKKDLYSMDEPVRVTHGVKPPPPQQQQQPLGPPTSLSYSHQPVYQDQQPYRQYDHPPYGYDGGGYAQPKPHNYDPHLHYDNRVPHYNEQWPTYDQQQQQQTSPQPPPASGFPQGHQPPPPPPMGYEPRSPYEDGPNRDFSPPQSQYDGVSPMGYDHRPRHPKPAPVRYEEPPPPPPPVSYDARSPFEPEPHAFPGNTHRSPDPPKQYYGDAAMRPLYNPGPPNRVYKHEPVNSEPPVPPPKPESVLSPGEPPHSAASKPLPPPPRDDDDDDDDDDDPAMKPQSVLNRVKMFENKRSVSVDRAKDAPDVTGIRPTDLPKPASTPGPVLKANSLSNLEQDKPSYRAPEPQKPQTRVGDDVVRSNHYDPDDDEEYYRKQLSFFDRRSFDSKVMNPPSAGVNRFHEPPKPPQTQIAYPFARTESVEKVSPVDKRYEPLPPVNPSPAPYSQNTPVAPPTSLPKLTNIEVNSLPDPHSSPKAKPDLSALRAPTRDEPIQSSYLPPKSTINGMDAPPKTLGVPTSYNRYVPKPYTSSARPFERKFESPKFNHNLLPNDTQSKPSVNNNLKPQISPQPQDTDSGVDTFTRTMDNRPKYQHNNINAIPKAVPVSPSALDDDEEDEGHTVVATARGIFNCNGGVLSSIETGVSIIIPQGAIPDSVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKCWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_009298130.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tight junction protein 1b (tjp1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298130.2

ORF Insert Sequence:

ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
TCCCGCAAGA ATGAAGCAGA ATACGGGCTT CGCCTGGCCA GCCACATCTT CGTGAAGGAC 
ATTTCCCCCG AGAGCCTGGC AGCCCGAGAC GGAAACATCC AAGAGGGAGA CGTGGTGCTT 
AAGATTAATG GCACGGTAAC AGAGAATTTG TCCCTGATCG ATGCTAAGAA GCTGATCGAG 
AGGTCAAAGG GAAAGCTGAA GATGGTGGTG CAGAGAGACG AGCGGGCGAC GCTGCTGAAC 
ATCCCCGATC TGGACGACAG CATACCTTCT GCTAATGCCT CGGACAGAGA TGATATTTCA 
GACATCCACT CCGTGGCCTC TGATCATTCC AACCGATCCC ACGACCGGAA GAGGAGCAGC 
CGCTCTCGTT CTCCAGACCG CCGCTCCGAA CCCTCAGACC ACTCCAGACA CTCTCCACCA 
CAGATCAGCA ACGGCAGGGC CACAGGGCCG ACGGCTCAGA GAACGCGGCC AGTTCACAGG 
AGCCGTGATG AAGACAGGAT CTCCAAGGCC GGGCCGCTTC CTCTACCTGC TAAGATGGTG 
GAGGAGATGC CGAAAGAGCA GAGCGCAGCC AGAGAAACAG AGAAACAGCT CCCCCCTCTG 
CCAGAGCCCA AACCTGTGTA CGCTCAGCCG GGCCAGCCGG ACGTGGACCT GCCTGTTAGT 
CCGGCAGATG CTCCTGTGCC CAGCGTCACA CATGATGACA GCATCTTACG GCCGAGCATG 
AAGCTGGTGA AGTTCAAGAA GGGCGAGAGC GTGGGCTTGA GGCTCGCTGG GGGGAACGAC 
GTTGGGATAT TTGTGGCGGG AGTTCTGGAG GACAGTCCTG CTGCTAAAGA GGGCCTGGAA 
GAGGGAGACC AGATTCTCAG GGTAAATAAT GTGGATTTTG CAAATATCAT CCGAGAGGAG 
GCAGTGCTGT TCCTGCTGGA TCTGCCCAAA GGAGAGGAGG TCACCATCCT GGCCCAGAAG 
AAGAAAGACG TGTACCGCCG CATTGTAGAG TCTGACGTGG GAGACTCGTT CTACATCAGG 
ACTCATTTTG AATATGAGAA GGAGTCGCCC TATGGCCTGA GCTTCAATAA GGGGGAGGTC 
TTCAGAGTCG TCGACACCCT TTACAATGGC AAACTGGGCT CCTGGCTCGC CATCCGCATC 
GGCAAAAACC ACCAGGAGGT GGAGCGGGGC ATCATCCCCA ACAAGAACAG GGCTGAGCAG 
TTATCAAGTG TCCAGTACAC TCTCCCAAAG ACTCCAGGAG GGGATCGCGC CGACTTCTGG 
AGATTCAGAG GCCTCCGCAG CTCCAAACGA AACCTGAGGA AGAGCAGAGA AGACCTGTCT 
GCTCAGCCGG TCCAAACCAA ATTCCCTGCT TATGAAAGAG TGGTGCTTAG AGAAGCTGGT 
TTCTTGAGGC CAGTGGTCAT TTTCGGACCG ATCGCGGATG TGGCTAGAGA GAAGCTGTCC 
AGAGAGGAGA CTGACCTCTT CGAACTTGCA AAGAGTGAAC CCAGAGATGC GGGCACAGAC 
CAGCGCAGCT CCGGAATCAT CCGTCTCCAT ACCATTAAAC AGATCATTGA CCGAGACAAG 
CACGCGGTGC TGGACATCAC CCCTAATGCG GTGGACCGTC TGAACTACGC TCAGTGGTAC 
CCCATCGTGG TGTTTCTGAA TCCAGACAGC AAACAGGGCG TCAAGAACAT GAGGACCAGG 
CTCTGTCCCG AGTCCAGGAA GAGCGCCCGA AAGCTCTACG AGCGTGCTCT CAAACTGAGG 
AAGAACAACC ATCACCTTTT CACCACCACT ATTAACTTGA ACAACATGAA TGACGGGTGG 
TACGGTGCTT TGAAAGAGAG CATCCAGCAG CAGCAGAACC AGTTGGTCTG GGTCTCAGAG 
GGCAAGGCCG ACGGCGCTCC TGACGATGAC CTTGACATCC ATGACGACCG CCTCTCCTAC 
CTCTCGGCTC CGGGCAGCGA GTACAGCATG TACAGCACCG ACAGCCGCCA CACGTCCGAC 
TATGAGGACA CCGACACGGA GGGTGGTGCC TACACCGACC AGGAGCTGGA CGAGACCATG 
AACGATGAGG TGGGCCTGCC TAGCGAGCCT GCCATCACAC GCTCATCTGA GCCTGTGCGA 
GAGGACCCGC CAGTCATTCA GGACGCAGCT GGATACCCCA GCTACCAGCA TGCGGTACCG 
CAACCTGAAG CGGTGAGCCG CATTGACCCG GCCGGGTTTA AGATGGCAGC GCCGCAGCAG 
CAAGCTGAGG CTGCTCTGGC CTCGCCGCCC CCTCCCTCTG CAGCGGTCCA GCCCACCGCA 
ACACTAGCGG GTACGAACCC CGAGGAGCCG CCTGGCACCC CTCAGGCCGA CTCCCTTAAC 
AGCCCCGTCC CTGTTCCCGA CCCCCAGCCC GAACCTGAGC TCGCTCAGCC CCCAACACAC 
GACCCCCACC AGTCGCTTCC TCCTGGCCCA GATCCAAAGA TGTATAAAAA GGATCTGTAC 
AGCATGGACG AGCCGGTGCG AGTGACCCAC GGCGTGAAGC CTCCCCCTCC CCAGCAGCAG 
CAGCAGCCTT TAGGCCCCCC AACCTCGCTC TCCTACAGCC ACCAGCCTGT CTACCAGGAC 
CAACAGCCAT ACCGCCAGTA CGACCACCCG CCTTATGGCT ATGACGGTGG CGGCTACGCA 
CAACCAAAGC CTCACAACTA CGACCCGCAC CTGCACTACG ACAACCGTGT GCCTCACTAT 
AACGAGCAGT GGCCCACGTA CGACCAGCAG CAGCAGCAGC AGACTTCCCC CCAGCCGCCA 
CCTGCGTCCG GCTTCCCTCA GGGCCACCAG CCTCCGCCTC CACCACCGAT GGGATATGAG 
CCACGCTCCC CATACGAGGA CGGACCTAAC AGGGATTTCA GCCCTCCTCA GTCCCAGTAT 
GACGGTGTGT CCCCAATGGG CTACGATCAC CGGCCGCGGC ACCCCAAACC TGCACCGGTC 
CGATATGAAG AGCCTCCACC TCCACCTCCT CCGGTGTCCT ACGATGCACG GTCACCTTTC 
GAACCCGAGC CGCATGCTTT CCCAGGCAAC ACACATCGCT CGCCAGACCC ACCGAAACAG 
TATTATGGAG ACGCCGCTAT GAGGCCCTTG TACAACCCTG GACCTCCGAA CAGAGTGTAC 
AAACATGAGC CGGTGAACTC TGAGCCTCCT GTACCGCCTC CCAAACCTGA ATCTGTCCTG 
TCTCCAGGAG AACCGCCCCA TTCTGCAGCT TCCAAACCAT TGCCGCCTCC TCCCAGAGAT 
GATGACGATG ATGATGACGA TGATGATGAC CCTGCCATGA AGCCCCAGTC TGTGCTCAAC 
AGGGTCAAAA TGTTCGAAAA CAAACGCTCT GTGTCGGTAG ACCGAGCCAA GGACGCACCT 
GACGTCACTG GAATTAGGCC CACAGATCTC CCCAAACCTG CGAGCACCCC TGGACCTGTG 
CTCAAAGCCA ACTCTCTCAG CAACCTGGAG CAGGACAAAC CCTCCTACAG AGCTCCAGAG 
CCGCAGAAAC CTCAGACGCG TGTTGGGGAC GATGTGGTTC GCTCCAACCA CTACGACCCT 
GATGATGATG AAGAGTACTA CAGAAAACAG CTCTCATTTT TCGACCGCCG CAGTTTCGAC 
AGTAAGGTCA TGAATCCACC CAGCGCTGGA GTAAACCGCT TCCATGAGCC GCCCAAACCA 
CCCCAGACAC AGATCGCTTA TCCTTTCGCT AGGACGGAGT CTGTGGAGAA GGTGAGTCCT 
GTGGACAAGA GATACGAGCC TCTGCCGCCA GTCAACCCTT CACCTGCTCC ATACAGCCAG 
AACACACCTG TCGCTCCGCC CACATCCCTG CCCAAACTCA CCAACATCGA GGTGAACTCT 
CTGCCAGATC CTCACAGCTC TCCCAAAGCC AAACCGGACC TGTCTGCTCT GAGAGCTCCG 
ACCCGGGACG AGCCCATCCA GAGCAGCTAC TTGCCCCCCA AGTCCACCAT CAATGGCATG 
GATGCTCCTC CCAAAACACT CGGCGTGCCG ACCAGCTACA ACCGCTACGT CCCCAAGCCT 
TACACCAGCT CGGCGCGACC CTTCGAGCGC AAGTTCGAGA GTCCGAAATT CAACCACAAC 
CTGCTTCCCA ACGACACGCA GTCCAAACCC AGTGTCAACA ACAACCTGAA GCCTCAGATC 
TCCCCACAAC CCCAGGACAC GGACAGCGGC GTCGACACCT TCACTCGCAC CATGGACAAC 
AGGCCAAAAT ATCAGCACAA CAACATCAAC GCCATTCCCA AAGCCGTCCC AGTCAGCCCC 
AGTGCACTGG ATGATGATGA AGAAGATGAG GGACACACAG TTGTGGCCAC GGCGCGGGGC 
ATCTTTAACT GTAATGGCGG GGTGTTGAGC TCTATAGAGA CGGGCGTCAG CATCATTATC 
CCACAGGGAG CCATTCCTGA TAGTGTGGAG CAGGAGATCT ACTTCAAAGT GTGCCGGGAC 
AACAGCATCC TGCCTCCTCT GGATAAGGAG AAAGGTGAAA CTCTGCTGAG TCCGCTGGTG 
ATGTGTGGTC CTCACGGCCT GAAGTTCCTG AAGCCTGTGG AGCTGCGCTT GCCACACTGT 
GCGTCTATGA CCCCTGATGG TTGGTCTTTT GCTCTAAAAT CCTCCGACTC CTCGTCGGGT 
GACCCCAAAT GCTGGCAAAA CAAGTGTCTA CCAGGAGACC CCAATTACCT GGTGGGCGCA 
AACTGTGTGT CTGTGCTGAT CGACCACTTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp1b ( XM_009298130.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296405.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X6, mRNA.

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CloneID	ODa44620
Clone ID Related Accession (Same CDS sequence)	XM_009298130.2 , XM_009298130.3
Accession Version	XM_009298130.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6093bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tight junction protein ZO-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009298130.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
TCCCGCAAGA ATGAAGCAGA ATACGGGCTT CGCCTGGCCA GCCACATCTT CGTGAAGGAC 
ATTTCCCCCG AGAGCCTGGC AGCCCGAGAC GGAAACATCC AAGAGGGAGA CGTGGTGCTT 
AAGATTAATG GCACGGTAAC AGAGAATTTG TCCCTGATCG ATGCTAAGAA GCTGATCGAG 
AGGTCAAAGG GAAAGCTGAA GATGGTGGTG CAGAGAGACG AGCGGGCGAC GCTGCTGAAC 
ATCCCCGATC TGGACGACAG CATACCTTCT GCTAATGCCT CGGACAGAGA TGATATTTCA 
GACATCCACT CCGTGGCCTC TGATCATTCC AACCGATCCC ACGACCGGAA GAGGAGCAGC 
CGCTCTCGTT CTCCAGACCG CCGCTCCGAA CCCTCAGACC ACTCCAGACA CTCTCCACCA 
CAGATCAGCA ACGGCAGGGC CACAGGGCCG ACGGCTCAGA GAACGCGGCC AGTTCACAGG 
AGCCGTGATG AAGACAGGAT CTCCAAGGCC GGGCCGCTTC CTCTACCTGC TAAGATGGTG 
GAGGAGATGC CGAAAGAGCA GAGCGCAGCC AGAGAAACAG AGAAACAGCT CCCCCCTCTG 
CCAGAGCCCA AACCTGTGTA CGCTCAGCCG GGCCAGCCGG ACGTGGACCT GCCTGTTAGT 
CCGGCAGATG CTCCTGTGCC CAGCGTCACA CATGATGACA GCATCTTACG GCCGAGCATG 
AAGCTGGTGA AGTTCAAGAA GGGCGAGAGC GTGGGCTTGA GGCTCGCTGG GGGGAACGAC 
GTTGGGATAT TTGTGGCGGG AGTTCTGGAG GACAGTCCTG CTGCTAAAGA GGGCCTGGAA 
GAGGGAGACC AGATTCTCAG GGTAAATAAT GTGGATTTTG CAAATATCAT CCGAGAGGAG 
GCAGTGCTGT TCCTGCTGGA TCTGCCCAAA GGAGAGGAGG TCACCATCCT GGCCCAGAAG 
AAGAAAGACG TGTACCGCCG CATTGTAGAG TCTGACGTGG GAGACTCGTT CTACATCAGG 
ACTCATTTTG AATATGAGAA GGAGTCGCCC TATGGCCTGA GCTTCAATAA GGGGGAGGTC 
TTCAGAGTCG TCGACACCCT TTACAATGGC AAACTGGGCT CCTGGCTCGC CATCCGCATC 
GGCAAAAACC ACCAGGAGGT GGAGCGGGGC ATCATCCCCA ACAAGAACAG GGCTGAGCAG 
TTATCAAGTG TCCAGTACAC TCTCCCAAAG ACTCCAGGAG GGGATCGCGC CGACTTCTGG 
AGATTCAGAG GCCTCCGCAG CTCCAAACGA AACCTGAGGA AGAGCAGAGA AGACCTGTCT 
GCTCAGCCGG TCCAAACCAA ATTCCCTGCT TATGAAAGAG TGGTGCTTAG AGAAGCTGGT 
TTCTTGAGGC CAGTGGTCAT TTTCGGACCG ATCGCGGATG TGGCTAGAGA GAAGCTGTCC 
AGAGAGGAGA CTGACCTCTT CGAACTTGCA AAGAGTGAAC CCAGAGATGC GGGCACAGAC 
CAGCGCAGCT CCGGAATCAT CCGTCTCCAT ACCATTAAAC AGATCATTGA CCGAGACAAG 
CACGCGGTGC TGGACATCAC CCCTAATGCG GTGGACCGTC TGAACTACGC TCAGTGGTAC 
CCCATCGTGG TGTTTCTGAA TCCAGACAGC AAACAGGGCG TCAAGAACAT GAGGACCAGG 
CTCTGTCCCG AGTCCAGGAA GAGCGCCCGA AAGCTCTACG AGCGTGCTCT CAAACTGAGG 
AAGAACAACC ATCACCTTTT CACCACCACT ATTAACTTGA ACAACATGAA TGACGGGTGG 
TACGGTGCTT TGAAAGAGAG CATCCAGCAG CAGCAGAACC AGTTGGTCTG GGTCTCAGAG 
GGCAAGGCCG ACGGCGCTCC TGACGATGAC CTTGACATCC ATGACGACCG CCTCTCCTAC 
CTCTCGGCTC CGGGCAGCGA GTACAGCATG TACAGCACCG ACAGCCGCCA CACGTCCGAC 
TATGAGGACA CCGACACGGA GGGTGGTGCC TACACCGACC AGGAGCTGGA CGAGACCATG 
AACGATGAGG TGGGCCTGCC TAGCGAGCCT GCCATCACAC GCTCATCTGA GCCTGTGCGA 
GAGGACCCGC CAGTCATTCA GGACGCAGCT GGATACCCCA GCTACCAGCA TGCGGTACCG 
CAACCTGAAG CGGTGAGCCG CATTGACCCG GCCGGGTTTA AGATGGCAGC GCCGCAGCAG 
CAAGCTGAGG CTGCTCTGGC CTCGCCGCCC CCTCCCTCTG CAGCGGTCCA GCCCACCGCA 
ACACTAGCGG GTACGAACCC CGAGGAGCCG CCTGGCACCC CTCAGGCCGA CTCCCTTAAC 
AGCCCCGTCC CTGTTCCCGA CCCCCAGCCC GAACCTGAGC TCGCTCAGCC CCCAACACAC 
GACCCCCACC AGTCGCTTCC TCCTGGCCCA GATCCAAAGA TGTATAAAAA GGATCTGTAC 
AGCATGGACG AGCCGGTGCG AGTGACCCAC GGCGTGAAGC CTCCCCCTCC CCAGCAGCAG 
CAGCAGCCTT TAGGCCCCCC AACCTCGCTC TCCTACAGCC ACCAGCCTGT CTACCAGGAC 
CAACAGCCAT ACCGCCAGTA CGACCACCCG CCTTATGGCT ATGACGGTGG CGGCTACGCA 
CAACCAAAGC CTCACAACTA CGACCCGCAC CTGCACTACG ACAACCGTGT GCCTCACTAT 
AACGAGCAGT GGCCCACGTA CGACCAGCAG CAGCAGCAGC AGACTTCCCC CCAGCCGCCA 
CCTGCGTCCG GCTTCCCTCA GGGCCACCAG CCTCCGCCTC CACCACCGAT GGGATATGAG 
CCACGCTCCC CATACGAGGA CGGACCTAAC AGGGATTTCA GCCCTCCTCA GTCCCAGTAT 
GACGGTGTGT CCCCAATGGG CTACGATCAC CGGCCGCGGC ACCCCAAACC TGCACCGGTC 
CGATATGAAG AGCCTCCACC TCCACCTCCT CCGGTGTCCT ACGATGCACG GTCACCTTTC 
GAACCCGAGC CGCATGCTTT CCCAGGCAAC ACACATCGCT CGCCAGACCC ACCGAAACAG 
TATTATGGAG ACGCCGCTAT GAGGCCCTTG TACAACCCTG GACCTCCGAA CAGAGTGTAC 
AAACATGAGC CGGTGAACTC TGAGCCTCCT GTACCGCCTC CCAAACCTGA ATCTGTCCTG 
TCTCCAGGAG AACCGCCCCA TTCTGCAGCT TCCAAACCAT TGCCGCCTCC TCCCAGAGAT 
GATGACGATG ATGATGACGA TGATGATGAC CCTGCCATGA AGCCCCAGTC TGTGCTCAAC 
AGGGTCAAAA TGTTCGAAAA CAAACGCTCT GTGTCGGTAG ACCGAGCCAA GGACGCACCT 
GACGTCACTG GAATTAGGCC CACAGATCTC CCCAAACCTG CGAGCACCCC TGGACCTGTG 
CTCAAAGCCA ACTCTCTCAG CAACCTGGAG CAGGACAAAC CCTCCTACAG AGCTCCAGAG 
CCGCAGAAAC CTCAGACGCG TGTTGGGGAC GATGTGGTTC GCTCCAACCA CTACGACCCT 
GATGATGATG AAGAGTACTA CAGAAAACAG CTCTCATTTT TCGACCGCCG CAGTTTCGAC 
AGTAAGGTCA TGAATCCACC CAGCGCTGGA GTAAACCGCT TCCATGAGCC GCCCAAACCA 
CCCCAGACAC AGATCGCTTA TCCTTTCGCT AGGACGGAGT CTGTGGAGAA GGTGAGTCCT 
GTGGACAAGA GATACGAGCC TCTGCCGCCA GTCAACCCTT CACCTGCTCC ATACAGCCAG 
AACACACCTG TCGCTCCGCC CACATCCCTG CCCAAACTCA CCAACATCGA GGTGAACTCT 
CTGCCAGATC CTCACAGCTC TCCCAAAGCC AAACCGGACC TGTCTGCTCT GAGAGCTCCG 
ACCCGGGACG AGCCCATCCA GAGCAGCTAC TTGCCCCCCA AGTCCACCAT CAATGGCATG 
GATGCTCCTC CCAAAACACT CGGCGTGCCG ACCAGCTACA ACCGCTACGT CCCCAAGCCT 
TACACCAGCT CGGCGCGACC CTTCGAGCGC AAGTTCGAGA GTCCGAAATT CAACCACAAC 
CTGCTTCCCA ACGACACGCA GTCCAAACCC AGTGTCAACA ACAACCTGAA GCCTCAGATC 
TCCCCACAAC CCCAGGACAC GGACAGCGGC GTCGACACCT TCACTCGCAC CATGGACAAC 
AGGCCAAAAT ATCAGCACAA CAACATCAAC GCCATTCCCA AAGCCGTCCC AGTCAGCCCC 
AGTGCACTGG ATGATGATGA AGAAGATGAG GGACACACAG TTGTGGCCAC GGCGCGGGGC 
ATCTTTAACT GTAATGGCGG GGTGTTGAGC TCTATAGAGA CGGGCGTCAG CATCATTATC 
CCACAGGGAG CCATTCCTGA TAGTGTGGAG CAGGAGATCT ACTTCAAAGT GTGCCGGGAC 
AACAGCATCC TGCCTCCTCT GGATAAGGAG AAAGGTGAAA CTCTGCTGAG TCCGCTGGTG 
ATGTGTGGTC CTCACGGCCT GAAGTTCCTG AAGCCTGTGG AGCTGCGCTT GCCACACTGT 
GCGTCTATGA CCCCTGATGG TTGGTCTTTT GCTCTAAAAT CCTCCGACTC CTCGTCGGGT 
GACCCCAAAT GCTGGCAAAA CAAGTGTCTA CCAGGAGACC CCAATTACCT GGTGGGCGCA 
AACTGTGTGT CTGTGCTGAT CGACCACTTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296405.1
CDS	110..6202
Translation	MLLSVFGGPVEEVLAGLLKLKMFGSGSKKWFIKKNKRQRTKVQRKPASGRRNKRVSRVLKRHKEVIVKQLDVSKVLPTLVYERVFTLGEYKEILGQDSSGKRAELFLDLLSQKEPAAFSAFCGVLEEVCPHLLTCFLLDGTDGSAGENKKQGQTKPAHSQQNALSKGQDTVCFPPMYTDPLFDTRVFPKHDKDKYGPAVYYPKFNAYIPNPDKVSVLDESEGSGHGKPSLRRIKGRIHRSKSLDSIDLLDSNSAAMDETVIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGLLQENDRVVMVNAVSMDNVDHAYAVQQLRKSGKNAKITIRRKRKVQIPSGRPGERETMSEHDEDDDSYEDEIYESRSARSGPSAYSGGGGGGGGGGASGRRSGRGDRLSGRRERDRERSGSRERSLSPRSDRHSVSSNLPPRPAKVTLVKSRKNEAEYGLRLASHIFVKDISPESLAARDGNIQEGDVVLKINGTVTENLSLIDAKKLIERSKGKLKMVVQRDERATLLNIPDLDDSIPSANASDRDDISDIHSVASDHSNRSHDRKRSSRSRSPDRRSEPSDHSRHSPPQISNGRATGPTAQRTRPVHRSRDEDRISKAGPLPLPAKMVEEMPKEQSAARETEKQLPPLPEPKPVYAQPGQPDVDLPVSPADAPVPSVTHDDSILRPSMKLVKFKKGESVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFANIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHQEVERGIIPNKNRAEQLSSVQYTLPKTPGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVVIFGPIADVAREKLSREETDLFELAKSEPRDAGTDQRSSGIIRLHTIKQIIDRDKHAVLDITPNAVDRLNYAQWYPIVVFLNPDSKQGVKNMRTRLCPESRKSARKLYERALKLRKNNHHLFTTTINLNNMNDGWYGALKESIQQQQNQLVWVSEGKADGAPDDDLDIHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETMNDEVGLPSEPAITRSSEPVREDPPVIQDAAGYPSYQHAVPQPEAVSRIDPAGFKMAAPQQQAEAALASPPPPSAAVQPTATLAGTNPEEPPGTPQADSLNSPVPVPDPQPEPELAQPPTHDPHQSLPPGPDPKMYKKDLYSMDEPVRVTHGVKPPPPQQQQQPLGPPTSLSYSHQPVYQDQQPYRQYDHPPYGYDGGGYAQPKPHNYDPHLHYDNRVPHYNEQWPTYDQQQQQQTSPQPPPASGFPQGHQPPPPPPMGYEPRSPYEDGPNRDFSPPQSQYDGVSPMGYDHRPRHPKPAPVRYEEPPPPPPPVSYDARSPFEPEPHAFPGNTHRSPDPPKQYYGDAAMRPLYNPGPPNRVYKHEPVNSEPPVPPPKPESVLSPGEPPHSAASKPLPPPPRDDDDDDDDDDDPAMKPQSVLNRVKMFENKRSVSVDRAKDAPDVTGIRPTDLPKPASTPGPVLKANSLSNLEQDKPSYRAPEPQKPQTRVGDDVVRSNHYDPDDDEEYYRKQLSFFDRRSFDSKVMNPPSAGVNRFHEPPKPPQTQIAYPFARTESVEKVSPVDKRYEPLPPVNPSPAPYSQNTPVAPPTSLPKLTNIEVNSLPDPHSSPKAKPDLSALRAPTRDEPIQSSYLPPKSTINGMDAPPKTLGVPTSYNRYVPKPYTSSARPFERKFESPKFNHNLLPNDTQSKPSVNNNLKPQISPQPQDTDSGVDTFTRTMDNRPKYQHNNINAIPKAVPVSPSALDDDEEDEGHTVVATARGIFNCNGGVLSSIETGVSIIIPQGAIPDSVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKCWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_009298130.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tight junction protein 1b (tjp1b), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298130.3

ORF Insert Sequence:

ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
TCCCGCAAGA ATGAAGCAGA ATACGGGCTT CGCCTGGCCA GCCACATCTT CGTGAAGGAC 
ATTTCCCCCG AGAGCCTGGC AGCCCGAGAC GGAAACATCC AAGAGGGAGA CGTGGTGCTT 
AAGATTAATG GCACGGTAAC AGAGAATTTG TCCCTGATCG ATGCTAAGAA GCTGATCGAG 
AGGTCAAAGG GAAAGCTGAA GATGGTGGTG CAGAGAGACG AGCGGGCGAC GCTGCTGAAC 
ATCCCCGATC TGGACGACAG CATACCTTCT GCTAATGCCT CGGACAGAGA TGATATTTCA 
GACATCCACT CCGTGGCCTC TGATCATTCC AACCGATCCC ACGACCGGAA GAGGAGCAGC 
CGCTCTCGTT CTCCAGACCG CCGCTCCGAA CCCTCAGACC ACTCCAGACA CTCTCCACCA 
CAGATCAGCA ACGGCAGGGC CACAGGGCCG ACGGCTCAGA GAACGCGGCC AGTTCACAGG 
AGCCGTGATG AAGACAGGAT CTCCAAGGCC GGGCCGCTTC CTCTACCTGC TAAGATGGTG 
GAGGAGATGC CGAAAGAGCA GAGCGCAGCC AGAGAAACAG AGAAACAGCT CCCCCCTCTG 
CCAGAGCCCA AACCTGTGTA CGCTCAGCCG GGCCAGCCGG ACGTGGACCT GCCTGTTAGT 
CCGGCAGATG CTCCTGTGCC CAGCGTCACA CATGATGACA GCATCTTACG GCCGAGCATG 
AAGCTGGTGA AGTTCAAGAA GGGCGAGAGC GTGGGCTTGA GGCTCGCTGG GGGGAACGAC 
GTTGGGATAT TTGTGGCGGG AGTTCTGGAG GACAGTCCTG CTGCTAAAGA GGGCCTGGAA 
GAGGGAGACC AGATTCTCAG GGTAAATAAT GTGGATTTTG CAAATATCAT CCGAGAGGAG 
GCAGTGCTGT TCCTGCTGGA TCTGCCCAAA GGAGAGGAGG TCACCATCCT GGCCCAGAAG 
AAGAAAGACG TGTACCGCCG CATTGTAGAG TCTGACGTGG GAGACTCGTT CTACATCAGG 
ACTCATTTTG AATATGAGAA GGAGTCGCCC TATGGCCTGA GCTTCAATAA GGGGGAGGTC 
TTCAGAGTCG TCGACACCCT TTACAATGGC AAACTGGGCT CCTGGCTCGC CATCCGCATC 
GGCAAAAACC ACCAGGAGGT GGAGCGGGGC ATCATCCCCA ACAAGAACAG GGCTGAGCAG 
TTATCAAGTG TCCAGTACAC TCTCCCAAAG ACTCCAGGAG GGGATCGCGC CGACTTCTGG 
AGATTCAGAG GCCTCCGCAG CTCCAAACGA AACCTGAGGA AGAGCAGAGA AGACCTGTCT 
GCTCAGCCGG TCCAAACCAA ATTCCCTGCT TATGAAAGAG TGGTGCTTAG AGAAGCTGGT 
TTCTTGAGGC CAGTGGTCAT TTTCGGACCG ATCGCGGATG TGGCTAGAGA GAAGCTGTCC 
AGAGAGGAGA CTGACCTCTT CGAACTTGCA AAGAGTGAAC CCAGAGATGC GGGCACAGAC 
CAGCGCAGCT CCGGAATCAT CCGTCTCCAT ACCATTAAAC AGATCATTGA CCGAGACAAG 
CACGCGGTGC TGGACATCAC CCCTAATGCG GTGGACCGTC TGAACTACGC TCAGTGGTAC 
CCCATCGTGG TGTTTCTGAA TCCAGACAGC AAACAGGGCG TCAAGAACAT GAGGACCAGG 
CTCTGTCCCG AGTCCAGGAA GAGCGCCCGA AAGCTCTACG AGCGTGCTCT CAAACTGAGG 
AAGAACAACC ATCACCTTTT CACCACCACT ATTAACTTGA ACAACATGAA TGACGGGTGG 
TACGGTGCTT TGAAAGAGAG CATCCAGCAG CAGCAGAACC AGTTGGTCTG GGTCTCAGAG 
GGCAAGGCCG ACGGCGCTCC TGACGATGAC CTTGACATCC ATGACGACCG CCTCTCCTAC 
CTCTCGGCTC CGGGCAGCGA GTACAGCATG TACAGCACCG ACAGCCGCCA CACGTCCGAC 
TATGAGGACA CCGACACGGA GGGTGGTGCC TACACCGACC AGGAGCTGGA CGAGACCATG 
AACGATGAGG TGGGCCTGCC TAGCGAGCCT GCCATCACAC GCTCATCTGA GCCTGTGCGA 
GAGGACCCGC CAGTCATTCA GGACGCAGCT GGATACCCCA GCTACCAGCA TGCGGTACCG 
CAACCTGAAG CGGTGAGCCG CATTGACCCG GCCGGGTTTA AGATGGCAGC GCCGCAGCAG 
CAAGCTGAGG CTGCTCTGGC CTCGCCGCCC CCTCCCTCTG CAGCGGTCCA GCCCACCGCA 
ACACTAGCGG GTACGAACCC CGAGGAGCCG CCTGGCACCC CTCAGGCCGA CTCCCTTAAC 
AGCCCCGTCC CTGTTCCCGA CCCCCAGCCC GAACCTGAGC TCGCTCAGCC CCCAACACAC 
GACCCCCACC AGTCGCTTCC TCCTGGCCCA GATCCAAAGA TGTATAAAAA GGATCTGTAC 
AGCATGGACG AGCCGGTGCG AGTGACCCAC GGCGTGAAGC CTCCCCCTCC CCAGCAGCAG 
CAGCAGCCTT TAGGCCCCCC AACCTCGCTC TCCTACAGCC ACCAGCCTGT CTACCAGGAC 
CAACAGCCAT ACCGCCAGTA CGACCACCCG CCTTATGGCT ATGACGGTGG CGGCTACGCA 
CAACCAAAGC CTCACAACTA CGACCCGCAC CTGCACTACG ACAACCGTGT GCCTCACTAT 
AACGAGCAGT GGCCCACGTA CGACCAGCAG CAGCAGCAGC AGACTTCCCC CCAGCCGCCA 
CCTGCGTCCG GCTTCCCTCA GGGCCACCAG CCTCCGCCTC CACCACCGAT GGGATATGAG 
CCACGCTCCC CATACGAGGA CGGACCTAAC AGGGATTTCA GCCCTCCTCA GTCCCAGTAT 
GACGGTGTGT CCCCAATGGG CTACGATCAC CGGCCGCGGC ACCCCAAACC TGCACCGGTC 
CGATATGAAG AGCCTCCACC TCCACCTCCT CCGGTGTCCT ACGATGCACG GTCACCTTTC 
GAACCCGAGC CGCATGCTTT CCCAGGCAAC ACACATCGCT CGCCAGACCC ACCGAAACAG 
TATTATGGAG ACGCCGCTAT GAGGCCCTTG TACAACCCTG GACCTCCGAA CAGAGTGTAC 
AAACATGAGC CGGTGAACTC TGAGCCTCCT GTACCGCCTC CCAAACCTGA ATCTGTCCTG 
TCTCCAGGAG AACCGCCCCA TTCTGCAGCT TCCAAACCAT TGCCGCCTCC TCCCAGAGAT 
GATGACGATG ATGATGACGA TGATGATGAC CCTGCCATGA AGCCCCAGTC TGTGCTCAAC 
AGGGTCAAAA TGTTCGAAAA CAAACGCTCT GTGTCGGTAG ACCGAGCCAA GGACGCACCT 
GACGTCACTG GAATTAGGCC CACAGATCTC CCCAAACCTG CGAGCACCCC TGGACCTGTG 
CTCAAAGCCA ACTCTCTCAG CAACCTGGAG CAGGACAAAC CCTCCTACAG AGCTCCAGAG 
CCGCAGAAAC CTCAGACGCG TGTTGGGGAC GATGTGGTTC GCTCCAACCA CTACGACCCT 
GATGATGATG AAGAGTACTA CAGAAAACAG CTCTCATTTT TCGACCGCCG CAGTTTCGAC 
AGTAAGGTCA TGAATCCACC CAGCGCTGGA GTAAACCGCT TCCATGAGCC GCCCAAACCA 
CCCCAGACAC AGATCGCTTA TCCTTTCGCT AGGACGGAGT CTGTGGAGAA GGTGAGTCCT 
GTGGACAAGA GATACGAGCC TCTGCCGCCA GTCAACCCTT CACCTGCTCC ATACAGCCAG 
AACACACCTG TCGCTCCGCC CACATCCCTG CCCAAACTCA CCAACATCGA GGTGAACTCT 
CTGCCAGATC CTCACAGCTC TCCCAAAGCC AAACCGGACC TGTCTGCTCT GAGAGCTCCG 
ACCCGGGACG AGCCCATCCA GAGCAGCTAC TTGCCCCCCA AGTCCACCAT CAATGGCATG 
GATGCTCCTC CCAAAACACT CGGCGTGCCG ACCAGCTACA ACCGCTACGT CCCCAAGCCT 
TACACCAGCT CGGCGCGACC CTTCGAGCGC AAGTTCGAGA GTCCGAAATT CAACCACAAC 
CTGCTTCCCA ACGACACGCA GTCCAAACCC AGTGTCAACA ACAACCTGAA GCCTCAGATC 
TCCCCACAAC CCCAGGACAC GGACAGCGGC GTCGACACCT TCACTCGCAC CATGGACAAC 
AGGCCAAAAT ATCAGCACAA CAACATCAAC GCCATTCCCA AAGCCGTCCC AGTCAGCCCC 
AGTGCACTGG ATGATGATGA AGAAGATGAG GGACACACAG TTGTGGCCAC GGCGCGGGGC 
ATCTTTAACT GTAATGGCGG GGTGTTGAGC TCTATAGAGA CGGGCGTCAG CATCATTATC 
CCACAGGGAG CCATTCCTGA TAGTGTGGAG CAGGAGATCT ACTTCAAAGT GTGCCGGGAC 
AACAGCATCC TGCCTCCTCT GGATAAGGAG AAAGGTGAAA CTCTGCTGAG TCCGCTGGTG 
ATGTGTGGTC CTCACGGCCT GAAGTTCCTG AAGCCTGTGG AGCTGCGCTT GCCACACTGT 
GCGTCTATGA CCCCTGATGG TTGGTCTTTT GCTCTAAAAT CCTCCGACTC CTCGTCGGGT 
GACCCCAAAT GCTGGCAAAA CAAGTGTCTA CCAGGAGACC CCAATTACCT GGTGGGCGCA 
AACTGTGTGT CTGTGCTGAT CGACCACTTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp1b ( XM_021470728.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326403.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X7, mRNA.

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CloneID	ODa44621
Clone ID Related Accession (Same CDS sequence)	XM_021470728.1
Accession Version	XM_021470728.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6093bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tight junction protein ZO-1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
TCCCGCAAGA ATGAAGCATG TATGGCGTCA GGAAGTTCTC AGAAGGTGAC ATCAGCAGAA 
TACGGGCTTC GCCTGGCCAG CCACATCTTC GTGAAGGACA TTTCCCCCGA GAGCCTGGCA 
GCCCGAGACG GAAACATCCA AGAGGGAGAC GTGGTGCTTA AGATTAATGG CACGGTAACA 
GAGAATTTGT CCCTGATCGA TGCTAAGAAG CTGATCGAGA GGTCAAAGGG AAAGCTGAAG 
ATGGTGGTGC AGAGAGACGA GCGGGCGACG CTGCTGAACA TCCCCGATCT GGACGACAGC 
ATACCTTCTG CTAATGCCTC GGACAGAGAT GATATTTCAG ACATCCACTC CGTGGCCTCT 
GATCATTCCA ACCGATCCCA CGACCGGAAG AGGAGCAGCC GCTCTCGTTC TCCAGACCGC 
CGCTCCGAAC CCTCAGACCA CTCCAGACAC TCTCCACCAC AGATCAGCAA CGGCAGGGCC 
ACAGGGCCGA CGGCTCAGAG AACGCGGCCA GTTCACAGGA GCCGTGATGA AGACAGGATC 
TCCAAGGCCG GGCCGCTTCC TCTACCTGCT AAGATGGTGG AGGAGATGCC GAAAGAGCAG 
AGCGCAGCCA GAGAAACAGA GAAACAGCTC CCCCCTCTGC CAGAGCCCAA ACCTGTGTAC 
GCTCAGCCGG GCCAGCCGGA CGTGGACCTG CCTGTTAGTC CGGCAGATGC TCCTGTGCCC 
AGCGTCACAC ATGATGACAG CATCTTACGG CCGAGCATGA AGCTGGTGAA GTTCAAGAAG 
GGCGAGAGCG TGGGCTTGAG GCTCGCTGGG GGGAACGACG TTGGGATATT TGTGGCGGGA 
GTTCTGGAGG ACAGTCCTGC TGCTAAAGAG GGCCTGGAAG AGGGAGACCA GATTCTCAGG 
GTAAATAATG TGGATTTTGC AAATATCATC CGAGAGGAGG CAGTGCTGTT CCTGCTGGAT 
CTGCCCAAAG GAGAGGAGGT CACCATCCTG GCCCAGAAGA AGAAAGACGT GTACCGCCGC 
ATTGTAGAGT CTGACGTGGG AGACTCGTTC TACATCAGGA CTCATTTTGA ATATGAGAAG 
GAGTCGCCCT ATGGCCTGAG CTTCAATAAG GGGGAGGTCT TCAGAGTCGT CGACACCCTT 
TACAATGGCA AACTGGGCTC CTGGCTCGCC ATCCGCATCG GCAAAAACCA CCAGGAGGTG 
GAGCGGGGCA TCATCCCCAA CAAGAACAGG GCTGAGCAGT TATCAAGTGT CCAGTACACT 
CTCCCAAAGA CTCCAGGAGG GGATCGCGCC GACTTCTGGA GATTCAGAGG CCTCCGCAGC 
TCCAAACGAA ACCTGAGGAA GAGCAGAGAA GACCTGTCTG CTCAGCCGGT CCAAACCAAA 
TTCCCTGCTT ATGAAAGAGT GGTGCTTAGA GAAGCTGGTT TCTTGAGGCC AGTGGTCATT 
TTCGGACCGA TCGCGGATGT GGCTAGAGAG AAGCTGTCCA GAGAGGAGAC TGACCTCTTC 
GAACTTGCAA AGAGTGAACC CAGAGATGCG GGCACAGACC AGCGCAGCTC CGGAATCATC 
CGTCTCCATA CCATTAAACA GATCATTGAC CGAGACAAGC ACGCGGTGCT GGACATCACC 
CCTAATGCGG TGGACCGTCT GAACTACGCT CAGTGGTACC CCATCGTGGT GTTTCTGAAT 
CCAGACAGCA AACAGGGCGT CAAGAACATG AGGACCAGGC TCTGTCCCGA GTCCAGGAAG 
AGCGCCCGAA AGCTCTACGA GCGTGCTCTC AAACTGAGGA AGAACAACCA TCACCTTTTC 
ACCACCACTA TTAACTTGAA CAACATGAAT GACGGGTGGT ACGGTGCTTT GAAAGAGAGC 
ATCCAGCAGC AGCAGAACCA GTTGGTCTGG GTCTCAGAGG GCAAGGCCGA CGGCGCTCCT 
GACGATGACC TTGACATCCA TGACGACCGC CTCTCCTACC TCTCGGCTCC GGGCAGCGAG 
TACAGCATGT ACAGCACCGA CAGCCGCCAC ACGTCCGACT ATGAGGACAC CGACACGGAG 
GGTGGTGCCT ACACCGACCA GGAGCTGGAC GAGACCATGA ACGATGAGGT GGGCCTGCCT 
AGCGAGCCTG CCATCACACG CTCATCTGAG CCTGTGCGAG AGGACCCGCC AGTCATTCAG 
GACGCAGCTG GATACCCCAG CTACCAGCAT GCGGTACCGC AACCTGAAGC GGTGAGCCGC 
ATTGACCCGG CCGGGTTTAA GATGGCAGCG CCGCAGCAGC AAGCTGAGGC TGCTCTGGCC 
TCGCCGCCCC CTCCCTCTGC AGCGGTCCAG CCCACCGCAA CACTAGCGGG TACGAACCCC 
GAGGAGCCGC CTGGCACCCC TCAGGCCGAC TCCCTTAACA GCCCCGTCCC TGTTCCCGAC 
CCCCAGCCCG AACCTGAGCT CGCTCAGCCC CCAACACACG ACCCCCACCA GTCGCTTCCT 
CCTGGCCCAG ATCCAAAGAT GTATAAAAAG GATCTGTACA GCATGGACGA GCCGGTGCGA 
GTGACCCACG GCGTGAAGCC TCCCCCTCCC CAGCAGCAGC AGCAGCCTTT AGGCCCCCCA 
ACCTCGCTCT CCTACAGCCA CCAGCCTGTC TACCAGGACC AACAGCCATA CCGCCAGTAC 
GACCACCCGC CTTATGGCTA TGACGGTGGC GGCTACGCAC AACCAAAGCC TCACAACTAC 
GACCCGCACC TGCACTACGA CAACCGTGTG CCTCACTATA ACGAGCAGTG GCCCACGTAC 
GACCAGCAGC AGCAGCAGCA GACTTCCCCC CAGCCGCCAC CTGCGTCCGG CTTCCCTCAG 
GGCCACCAGC CTCCGCCTCC ACCACCGATG GGATATGAGC CACGCTCCCC ATACGAGGAC 
GGACCTAACA GGGATTTCAG CCCTCCTCAG TCCCAGTATG ACGGTGTGTC CCCAATGGGC 
TACGATCACC GGCCGCGGCA CCCCAAACCT GCACCGGTCC GATATGAAGA GCCTCCACCT 
CCACCTCCTC CGGTGTCCTA CGATGCACGG TCACCTTTCG AACCCGAGCC GCATGCTTTC 
CCAGGCAACA CACATCGCTC GCCAGACCCA CCGAAACAGT ATTATGGAGA CGCCGCTATG 
AGGCCCTTGT ACAACCCTGG ACCTCCGAAC AGAGTGTACA AACATGAGCC GGTGAACTCT 
GAGCCTCCTG TACCGCCTCC CAAACCTGAA TCTGTCCTGT CTCCAGGAGA ACCGCCCCAT 
TCTGCAGCTT CCAAACCATT GCCGCCTCCT CCCAGAGATG ATGACGATGA TGATGACGAT 
GATGATGACC CTGCCATGAA GCCCCAGTCT GTGCTCAACA GGGTCAAAAT GTTCGAAAAC 
AAACGCTCTG TGTCGGTAGA CCGAGCCAAG GACGCACCTG ACGTCACTGG AATTAGGCCC 
ACAGATCTCC CCAAACCTGC GAGCACCCCT GGACCTGTGC TCAAAGCCAA CTCTCTCAGC 
AACCTGGAGC AGGACAAACC CTCCTACAGA GCTCCAGAGC CGCAGAAACC TCAGACGCGT 
GTTGGGGACG ATGTGGTTCG CTCCAACCAC TACGACCCTG ATGATGATGA AGAGTACTAC 
AGAAAACAGC TCTCATTTTT CGACCGCCGC AGTTTCGACA GTAAGGTCAT GAATCCACCC 
AGCGCTGGAG TAAACCGCTT CCATGAGCCG CCCAAACCAC CCCAGACACA GATCGCTTAT 
CCTTTCGCTA GGACGGAGTC TGTGGAGAAG GTGAGTCCTG TGGACAAGAG ATACGAGCCT 
CTGCCGCCAG TCAACCCTTC ACCTGCTCCA TACAGCCAGA ACACACCTGT CGCTCCGCCC 
ACATCCCTGC CCAAACTCAC CAACATCGAG GTGAACTCTC TGCCAGATCC TCACAGCTCT 
CCCAAAGCCA AACCGGACCT GTCTGCTCTG AGAGCTCCGA CCCGGGACGA GCCCATCCAG 
AGCAGCTACT TGCCCCCCAA GTCCACCATC AATGGCATGG ATGCTCCTCC CAAAACACTC 
GGCGTGCCGA CCAGCTACAA CCGCTACGTC CCCAAGCCTT ACACCAGCTC GGCGCGACCC 
TTCGAGCGCA AGTTCGAGAG TCCGAAATTC AACCACAACC TGCTTCCCAA CGACACGCAG 
TCCAAACCCA GTGTCAACAA CAACCTGAAG CCTCAGATCT CCCCACAACC CCAGGACACG 
GACAGCGGCG TCGACACCTT CACTCGCACC ATGGACAACA GGCCAAAATA TCAGCACAAC 
AACATCAACG CCATTCCCAA AGCCGTCCCA GTCAGCCCCA GTGCACTGGA TGATGATGAA 
GAAGATGAGG GACACACAGT TGTGGCCACG GCGCGGGGCA TCTTTAACTG TAATGGCGGG 
GTGTTGAGCT CTATAGAGAC GGGCGTCAGC ATCATTATCC CACAGGGAGC CATTCCTGAT 
AGTGTGGAGC AGGAGATCTA CTTCAAAGTG TGCCGGGACA ACAGCATCCT GCCTCCTCTG 
GATAAGGAGA AAGGTGAAAC TCTGCTGAGT CCGCTGGTGA TGTGTGGTCC TCACGGCCTG 
AAGTTCCTGA AGCCTGTGGA GCTGCGCTTG CCACACTGTG CGTCTATGAC CCCTGATGGT 
GACCCCAAAT GCTGGCAAAA CAAGTGTCTA CCAGGAGACC CCAATTACCT GGTGGGCGCA 
AACTGTGTGT CTGTGCTGAT CGACCACTTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326403.1
CDS	110..6202
Translation	MLLSVFGGPVEEVLAGLLKLKMFGSGSKKWFIKKNKRQRTKVQRKPASGRRNKRVSRVLKRHKEVIVKQLDVSKVLPTLVYERVFTLGEYKEILGQDSSGKRAELFLDLLSQKEPAAFSAFCGVLEEVCPHLLTCFLLDGTDGSAGENKKQGQTKPAHSQQNALSKGQDTVCFPPMYTDPLFDTRVFPKHDKDKYGPAVYYPKFNAYIPNPDKVSVLDESEGSGHGKPSLRRIKGRIHRSKSLDSIDLLDSNSAAMDETVIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGLLQENDRVVMVNAVSMDNVDHAYAVQQLRKSGKNAKITIRRKRKVQIPSGRPGERETMSEHDEDDDSYEDEIYESRSARSGPSAYSGGGGGGGGGGASGRRSGRGDRLSGRRERDRERSGSRERSLSPRSDRHSVSSNLPPRPAKVTLVKSRKNEACMASGSSQKVTSAEYGLRLASHIFVKDISPESLAARDGNIQEGDVVLKINGTVTENLSLIDAKKLIERSKGKLKMVVQRDERATLLNIPDLDDSIPSANASDRDDISDIHSVASDHSNRSHDRKRSSRSRSPDRRSEPSDHSRHSPPQISNGRATGPTAQRTRPVHRSRDEDRISKAGPLPLPAKMVEEMPKEQSAARETEKQLPPLPEPKPVYAQPGQPDVDLPVSPADAPVPSVTHDDSILRPSMKLVKFKKGESVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFANIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHQEVERGIIPNKNRAEQLSSVQYTLPKTPGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVVIFGPIADVAREKLSREETDLFELAKSEPRDAGTDQRSSGIIRLHTIKQIIDRDKHAVLDITPNAVDRLNYAQWYPIVVFLNPDSKQGVKNMRTRLCPESRKSARKLYERALKLRKNNHHLFTTTINLNNMNDGWYGALKESIQQQQNQLVWVSEGKADGAPDDDLDIHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETMNDEVGLPSEPAITRSSEPVREDPPVIQDAAGYPSYQHAVPQPEAVSRIDPAGFKMAAPQQQAEAALASPPPPSAAVQPTATLAGTNPEEPPGTPQADSLNSPVPVPDPQPEPELAQPPTHDPHQSLPPGPDPKMYKKDLYSMDEPVRVTHGVKPPPPQQQQQPLGPPTSLSYSHQPVYQDQQPYRQYDHPPYGYDGGGYAQPKPHNYDPHLHYDNRVPHYNEQWPTYDQQQQQQTSPQPPPASGFPQGHQPPPPPPMGYEPRSPYEDGPNRDFSPPQSQYDGVSPMGYDHRPRHPKPAPVRYEEPPPPPPPVSYDARSPFEPEPHAFPGNTHRSPDPPKQYYGDAAMRPLYNPGPPNRVYKHEPVNSEPPVPPPKPESVLSPGEPPHSAASKPLPPPPRDDDDDDDDDDDPAMKPQSVLNRVKMFENKRSVSVDRAKDAPDVTGIRPTDLPKPASTPGPVLKANSLSNLEQDKPSYRAPEPQKPQTRVGDDVVRSNHYDPDDDEEYYRKQLSFFDRRSFDSKVMNPPSAGVNRFHEPPKPPQTQIAYPFARTESVEKVSPVDKRYEPLPPVNPSPAPYSQNTPVAPPTSLPKLTNIEVNSLPDPHSSPKAKPDLSALRAPTRDEPIQSSYLPPKSTINGMDAPPKTLGVPTSYNRYVPKPYTSSARPFERKFESPKFNHNLLPNDTQSKPSVNNNLKPQISPQPQDTDSGVDTFTRTMDNRPKYQHNNINAIPKAVPVSPSALDDDEEDEGHTVVATARGIFNCNGGVLSSIETGVSIIIPQGAIPDSVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGDPKCWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_021470728.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tight junction protein 1b (tjp1b), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470728.1

ORF Insert Sequence:

ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
TCCCGCAAGA ATGAAGCATG TATGGCGTCA GGAAGTTCTC AGAAGGTGAC ATCAGCAGAA 
TACGGGCTTC GCCTGGCCAG CCACATCTTC GTGAAGGACA TTTCCCCCGA GAGCCTGGCA 
GCCCGAGACG GAAACATCCA AGAGGGAGAC GTGGTGCTTA AGATTAATGG CACGGTAACA 
GAGAATTTGT CCCTGATCGA TGCTAAGAAG CTGATCGAGA GGTCAAAGGG AAAGCTGAAG 
ATGGTGGTGC AGAGAGACGA GCGGGCGACG CTGCTGAACA TCCCCGATCT GGACGACAGC 
ATACCTTCTG CTAATGCCTC GGACAGAGAT GATATTTCAG ACATCCACTC CGTGGCCTCT 
GATCATTCCA ACCGATCCCA CGACCGGAAG AGGAGCAGCC GCTCTCGTTC TCCAGACCGC 
CGCTCCGAAC CCTCAGACCA CTCCAGACAC TCTCCACCAC AGATCAGCAA CGGCAGGGCC 
ACAGGGCCGA CGGCTCAGAG AACGCGGCCA GTTCACAGGA GCCGTGATGA AGACAGGATC 
TCCAAGGCCG GGCCGCTTCC TCTACCTGCT AAGATGGTGG AGGAGATGCC GAAAGAGCAG 
AGCGCAGCCA GAGAAACAGA GAAACAGCTC CCCCCTCTGC CAGAGCCCAA ACCTGTGTAC 
GCTCAGCCGG GCCAGCCGGA CGTGGACCTG CCTGTTAGTC CGGCAGATGC TCCTGTGCCC 
AGCGTCACAC ATGATGACAG CATCTTACGG CCGAGCATGA AGCTGGTGAA GTTCAAGAAG 
GGCGAGAGCG TGGGCTTGAG GCTCGCTGGG GGGAACGACG TTGGGATATT TGTGGCGGGA 
GTTCTGGAGG ACAGTCCTGC TGCTAAAGAG GGCCTGGAAG AGGGAGACCA GATTCTCAGG 
GTAAATAATG TGGATTTTGC AAATATCATC CGAGAGGAGG CAGTGCTGTT CCTGCTGGAT 
CTGCCCAAAG GAGAGGAGGT CACCATCCTG GCCCAGAAGA AGAAAGACGT GTACCGCCGC 
ATTGTAGAGT CTGACGTGGG AGACTCGTTC TACATCAGGA CTCATTTTGA ATATGAGAAG 
GAGTCGCCCT ATGGCCTGAG CTTCAATAAG GGGGAGGTCT TCAGAGTCGT CGACACCCTT 
TACAATGGCA AACTGGGCTC CTGGCTCGCC ATCCGCATCG GCAAAAACCA CCAGGAGGTG 
GAGCGGGGCA TCATCCCCAA CAAGAACAGG GCTGAGCAGT TATCAAGTGT CCAGTACACT 
CTCCCAAAGA CTCCAGGAGG GGATCGCGCC GACTTCTGGA GATTCAGAGG CCTCCGCAGC 
TCCAAACGAA ACCTGAGGAA GAGCAGAGAA GACCTGTCTG CTCAGCCGGT CCAAACCAAA 
TTCCCTGCTT ATGAAAGAGT GGTGCTTAGA GAAGCTGGTT TCTTGAGGCC AGTGGTCATT 
TTCGGACCGA TCGCGGATGT GGCTAGAGAG AAGCTGTCCA GAGAGGAGAC TGACCTCTTC 
GAACTTGCAA AGAGTGAACC CAGAGATGCG GGCACAGACC AGCGCAGCTC CGGAATCATC 
CGTCTCCATA CCATTAAACA GATCATTGAC CGAGACAAGC ACGCGGTGCT GGACATCACC 
CCTAATGCGG TGGACCGTCT GAACTACGCT CAGTGGTACC CCATCGTGGT GTTTCTGAAT 
CCAGACAGCA AACAGGGCGT CAAGAACATG AGGACCAGGC TCTGTCCCGA GTCCAGGAAG 
AGCGCCCGAA AGCTCTACGA GCGTGCTCTC AAACTGAGGA AGAACAACCA TCACCTTTTC 
ACCACCACTA TTAACTTGAA CAACATGAAT GACGGGTGGT ACGGTGCTTT GAAAGAGAGC 
ATCCAGCAGC AGCAGAACCA GTTGGTCTGG GTCTCAGAGG GCAAGGCCGA CGGCGCTCCT 
GACGATGACC TTGACATCCA TGACGACCGC CTCTCCTACC TCTCGGCTCC GGGCAGCGAG 
TACAGCATGT ACAGCACCGA CAGCCGCCAC ACGTCCGACT ATGAGGACAC CGACACGGAG 
GGTGGTGCCT ACACCGACCA GGAGCTGGAC GAGACCATGA ACGATGAGGT GGGCCTGCCT 
AGCGAGCCTG CCATCACACG CTCATCTGAG CCTGTGCGAG AGGACCCGCC AGTCATTCAG 
GACGCAGCTG GATACCCCAG CTACCAGCAT GCGGTACCGC AACCTGAAGC GGTGAGCCGC 
ATTGACCCGG CCGGGTTTAA GATGGCAGCG CCGCAGCAGC AAGCTGAGGC TGCTCTGGCC 
TCGCCGCCCC CTCCCTCTGC AGCGGTCCAG CCCACCGCAA CACTAGCGGG TACGAACCCC 
GAGGAGCCGC CTGGCACCCC TCAGGCCGAC TCCCTTAACA GCCCCGTCCC TGTTCCCGAC 
CCCCAGCCCG AACCTGAGCT CGCTCAGCCC CCAACACACG ACCCCCACCA GTCGCTTCCT 
CCTGGCCCAG ATCCAAAGAT GTATAAAAAG GATCTGTACA GCATGGACGA GCCGGTGCGA 
GTGACCCACG GCGTGAAGCC TCCCCCTCCC CAGCAGCAGC AGCAGCCTTT AGGCCCCCCA 
ACCTCGCTCT CCTACAGCCA CCAGCCTGTC TACCAGGACC AACAGCCATA CCGCCAGTAC 
GACCACCCGC CTTATGGCTA TGACGGTGGC GGCTACGCAC AACCAAAGCC TCACAACTAC 
GACCCGCACC TGCACTACGA CAACCGTGTG CCTCACTATA ACGAGCAGTG GCCCACGTAC 
GACCAGCAGC AGCAGCAGCA GACTTCCCCC CAGCCGCCAC CTGCGTCCGG CTTCCCTCAG 
GGCCACCAGC CTCCGCCTCC ACCACCGATG GGATATGAGC CACGCTCCCC ATACGAGGAC 
GGACCTAACA GGGATTTCAG CCCTCCTCAG TCCCAGTATG ACGGTGTGTC CCCAATGGGC 
TACGATCACC GGCCGCGGCA CCCCAAACCT GCACCGGTCC GATATGAAGA GCCTCCACCT 
CCACCTCCTC CGGTGTCCTA CGATGCACGG TCACCTTTCG AACCCGAGCC GCATGCTTTC 
CCAGGCAACA CACATCGCTC GCCAGACCCA CCGAAACAGT ATTATGGAGA CGCCGCTATG 
AGGCCCTTGT ACAACCCTGG ACCTCCGAAC AGAGTGTACA AACATGAGCC GGTGAACTCT 
GAGCCTCCTG TACCGCCTCC CAAACCTGAA TCTGTCCTGT CTCCAGGAGA ACCGCCCCAT 
TCTGCAGCTT CCAAACCATT GCCGCCTCCT CCCAGAGATG ATGACGATGA TGATGACGAT 
GATGATGACC CTGCCATGAA GCCCCAGTCT GTGCTCAACA GGGTCAAAAT GTTCGAAAAC 
AAACGCTCTG TGTCGGTAGA CCGAGCCAAG GACGCACCTG ACGTCACTGG AATTAGGCCC 
ACAGATCTCC CCAAACCTGC GAGCACCCCT GGACCTGTGC TCAAAGCCAA CTCTCTCAGC 
AACCTGGAGC AGGACAAACC CTCCTACAGA GCTCCAGAGC CGCAGAAACC TCAGACGCGT 
GTTGGGGACG ATGTGGTTCG CTCCAACCAC TACGACCCTG ATGATGATGA AGAGTACTAC 
AGAAAACAGC TCTCATTTTT CGACCGCCGC AGTTTCGACA GTAAGGTCAT GAATCCACCC 
AGCGCTGGAG TAAACCGCTT CCATGAGCCG CCCAAACCAC CCCAGACACA GATCGCTTAT 
CCTTTCGCTA GGACGGAGTC TGTGGAGAAG GTGAGTCCTG TGGACAAGAG ATACGAGCCT 
CTGCCGCCAG TCAACCCTTC ACCTGCTCCA TACAGCCAGA ACACACCTGT CGCTCCGCCC 
ACATCCCTGC CCAAACTCAC CAACATCGAG GTGAACTCTC TGCCAGATCC TCACAGCTCT 
CCCAAAGCCA AACCGGACCT GTCTGCTCTG AGAGCTCCGA CCCGGGACGA GCCCATCCAG 
AGCAGCTACT TGCCCCCCAA GTCCACCATC AATGGCATGG ATGCTCCTCC CAAAACACTC 
GGCGTGCCGA CCAGCTACAA CCGCTACGTC CCCAAGCCTT ACACCAGCTC GGCGCGACCC 
TTCGAGCGCA AGTTCGAGAG TCCGAAATTC AACCACAACC TGCTTCCCAA CGACACGCAG 
TCCAAACCCA GTGTCAACAA CAACCTGAAG CCTCAGATCT CCCCACAACC CCAGGACACG 
GACAGCGGCG TCGACACCTT CACTCGCACC ATGGACAACA GGCCAAAATA TCAGCACAAC 
AACATCAACG CCATTCCCAA AGCCGTCCCA GTCAGCCCCA GTGCACTGGA TGATGATGAA 
GAAGATGAGG GACACACAGT TGTGGCCACG GCGCGGGGCA TCTTTAACTG TAATGGCGGG 
GTGTTGAGCT CTATAGAGAC GGGCGTCAGC ATCATTATCC CACAGGGAGC CATTCCTGAT 
AGTGTGGAGC AGGAGATCTA CTTCAAAGTG TGCCGGGACA ACAGCATCCT GCCTCCTCTG 
GATAAGGAGA AAGGTGAAAC TCTGCTGAGT CCGCTGGTGA TGTGTGGTCC TCACGGCCTG 
AAGTTCCTGA AGCCTGTGGA GCTGCGCTTG CCACACTGTG CGTCTATGAC CCCTGATGGT 
GACCCCAAAT GCTGGCAAAA CAAGTGTCTA CCAGGAGACC CCAATTACCT GGTGGGCGCA 
AACTGTGTGT CTGTGCTGAT CGACCACTTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp1b ( XM_009298132.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296407.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X2, mRNA.

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CloneID	ODa44622
Clone ID Related Accession (Same CDS sequence)	XM_009298132.2 , XM_009298132.3
Accession Version	XM_009298132.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6090bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tight junction protein ZO-1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298132.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
TCCCGCAAGA ATGAAGAATA CGGGCTTCGC CTGGCCAGCC ACATCTTCGT GAAGGACATT 
TCCCCCGAGA GCCTGGCAGC CCGAGACGGA AACATCCAAG AGGGAGACGT GGTGCTTAAG 
ATTAATGGCA CGGTAACAGA GAATTTGTCC CTGATCGATG CTAAGAAGCT GATCGAGAGG 
TCAAAGGGAA AGCTGAAGAT GGTGGTGCAG AGAGACGAGC GGGCGACGCT GCTGAACATC 
CCCGATCTGG ACGACAGCAT ACCTTCTGCT AATGCCTCGG ACAGAGATGA TATTTCAGAC 
ATCCACTCCG TGGCCTCTGA TCATTCCAAC CGATCCCACG ACCGGAAGAG GAGCAGCCGC 
TCTCGTTCTC CAGACCGCCG CTCCGAACCC TCAGACCACT CCAGACACTC TCCACCACAG 
ATCAGCAACG GCAGGGCCAC AGGGCCGACG GCTCAGAGAA CGCGGCCAGT TCACAGGAGC 
CGTGATGAAG ACAGGATCTC CAAGGCCGGG CCGCTTCCTC TACCTGCTAA GATGGTGGAG 
GAGATGCCGA AAGAGCAGAG CGCAGCCAGA GAAACAGAGA AACAGCTCCC CCCTCTGCCA 
GAGCCCAAAC CTGTGTACGC TCAGCCGGGC CAGCCGGACG TGGACCTGCC TGTTAGTCCG 
GCAGATGCTC CTGTGCCCAG CGTCACACAT GATGACAGCA TCTTACGGCC GAGCATGAAG 
CTGGTGAAGT TCAAGAAGGG CGAGAGCGTG GGCTTGAGGC TCGCTGGGGG GAACGACGTT 
GGGATATTTG TGGCGGGAGT TCTGGAGGAC AGTCCTGCTG CTAAAGAGGG CCTGGAAGAG 
GGAGACCAGA TTCTCAGGGT AAATAATGTG GATTTTGCAA ATATCATCCG AGAGGAGGCA 
GTGCTGTTCC TGCTGGATCT GCCCAAAGGA GAGGAGGTCA CCATCCTGGC CCAGAAGAAG 
AAAGACGTGT ACCGCCGCAT TGTAGAGTCT GACGTGGGAG ACTCGTTCTA CATCAGGACT 
CATTTTGAAT ATGAGAAGGA GTCGCCCTAT GGCCTGAGCT TCAATAAGGG GGAGGTCTTC 
AGAGTCGTCG ACACCCTTTA CAATGGCAAA CTGGGCTCCT GGCTCGCCAT CCGCATCGGC 
AAAAACCACC AGGAGGTGGA GCGGGGCATC ATCCCCAACA AGAACAGGGC TGAGCAGTTA 
TCAAGTGTCC AGTACACTCT CCCAAAGACT CCAGGAGGGG ATCGCGCCGA CTTCTGGAGA 
TTCAGAGGCC TCCGCAGCTC CAAACGAAAC CTGAGGAAGA GCAGAGAAGA CCTGTCTGCT 
CAGCCGGTCC AAACCAAATT CCCTGCTTAT GAAAGAGTGG TGCTTAGAGA AGCTGGTTTC 
TTGAGGCCAG TGGTCATTTT CGGACCGATC GCGGATGTGG CTAGAGAGAA GCTGTCCAGA 
GAGGAGACTG ACCTCTTCGA ACTTGCAAAG AGTGAACCCA GAGATGCGGG CACAGACCAG 
CGCAGCTCCG GAATCATCCG TCTCCATACC ATTAAACAGA TCATTGACCG AGACAAGCAC 
GCGGTGCTGG ACATCACCCC TAATGCGGTG GACCGTCTGA ACTACGCTCA GTGGTACCCC 
ATCGTGGTGT TTCTGAATCC AGACAGCAAA CAGGGCGTCA AGAACATGAG GACCAGGCTC 
TGTCCCGAGT CCAGGAAGAG CGCCCGAAAG CTCTACGAGC GTGCTCTCAA ACTGAGGAAG 
AACAACCATC ACCTTTTCAC CACCACTATT AACTTGAACA ACATGAATGA CGGGTGGTAC 
GGTGCTTTGA AAGAGAGCAT CCAGCAGCAG CAGAACCAGT TGGTCTGGGT CTCAGAGGGC 
AAGGCCGACG GCGCTCCTGA CGATGACCTT GACATCCATG ACGACCGCCT CTCCTACCTC 
TCGGCTCCGG GCAGCGAGTA CAGCATGTAC AGCACCGACA GCCGCCACAC GTCCGACTAT 
GAGGACACCG ACACGGAGGG TGGTGCCTAC ACCGACCAGG AGCTGGACGA GACCATGAAC 
GATGAGGTGG GCCTGCCTAG CGAGCCTGCC ATCACACGCT CATCTGAGCC TGTGCGAGAG 
GACCCGCCAG TCATTCAGGA CGCAGCTGGA TACCCCAGCT ACCAGCATGC GGTACCGCAA 
CCTGAAGCGG TGAGCCGCAT TGACCCGGCC GGGTTTAAGA TGGCAGCGCC GCAGCAGCAA 
GCTGAGGCTG CTCTGGCCTC GCCGCCCCCT CCCTCTGCAG CGGTCCAGCC CACCGCAACA 
CTAGCGGGTA CGAACCCCGA GGAGCCGCCT GGCACCCCTC AGGCCGACTC CCTTAACAGC 
CCCGTCCCTG TTCCCGACCC CCAGCCCGAA CCTGAGCTCG CTCAGCCCCC AACACACGAC 
CCCCACCAGT CGCTTCCTCC TGGCCCAGAT CCAAAGATGT ATAAAAAGGA TCTGTACAGC 
ATGGACGAGC CGGTGCGAGT GACCCACGGC GTGAAGCCTC CCCCTCCCCA GCAGCAGCAG 
CAGCCTTTAG GCCCCCCAAC CTCGCTCTCC TACAGCCACC AGCCTGTCTA CCAGGACCAA 
CAGCCATACC GCCAGTACGA CCACCCGCCT TATGGCTATG ACGGTGGCGG CTACGCACAA 
CCAAAGCCTC ACAACTACGA CCCGCACCTG CACTACGACA ACCGTGTGCC TCACTATAAC 
GAGCAGTGGC CCACGTACGA CCAGCAGCAG CAGCAGCAGA CTTCCCCCCA GCCGCCACCT 
GCGTCCGGCT TCCCTCAGGG CCACCAGCCT CCGCCTCCAC CACCGATGGG ATATGAGCCA 
CGCTCCCCAT ACGAGGACGG ACCTAACAGG GATTTCAGCC CTCCTCAGTC CCAGTATGAC 
GGTGTGTCCC CAATGGGCTA CGATCACCGG CCGCGGCACC CCAAACCTGC ACCGGTCCGA 
TATGAAGAGC CTCCACCTCC ACCTCCTCCG GTGTCCTACG ATGCACGGTC ACCTTTCGAA 
CCCGAGCCGC ATGCTTTCCC AGGCAACACA CATCGCTCGC CAGACCCACC GAAACAGTAT 
TATGGAGACG CCGCTATGAG GCCCTTGTAC AACCCTGGAC CTCCGAACAG AGTGTACAAA 
CATGAGCCGG TGAACTCTGA GCCTCCTGTA CCGCCTCCCA AACCTGAATC TGTCCTGTCT 
CCAGGAGAAC CGCCCCATTC TGCAGCTTCC AAACCATTGC CGCCTCCTCC CAGAGATGAT 
GACGATGATG ATGACGATGA TGATGACCCT GCCATGAAGC CCCAGTCTGT GCTCAACAGG 
GTCAAAATGT TCGAAAACAA ACGCTCTGTG TCGGTAGACC GAGCCAAGGA CGCACCTGAC 
GTCACTGGAA TTAGGCCCAC AGATCTCCCC AAACCTGCGA GCACCCCTGG ACCTGTGCTC 
AAAGCCAACT CTCTCAGCAA CCTGGAGCAG GACAAACCCT CCTACAGAGC TCCAGAGCCG 
CAGAAACCTC AGACGCGTGT TGGGGACGAT GTGGTTCGCT CCAACCACTA CGACCCTGAT 
GATGATGAAG AGTACTACAG AAAACAGCTC TCATTTTTCG ACCGCCGCAG TTTCGACAGT 
AAGGTCATGA ATCCACCCAG CGCTGGAGTA AACCGCTTCC ATGAGCCGCC CAAACCACCC 
CAGACACAGA TCGCTTATCC TTTCGCTAGG ACGGAGTCTG TGGAGAAGGT GAGTCCTGTG 
GACAAGAGAT ACGAGCCTCT GCCGCCAGTC AACCCTTCAC CTGCTCCATA CAGCCAGAAC 
ACACCTGTCG CTCCGCCCAC ATCCCTGCCC AAACTCACCA ACATCGAGGT GAACTCTCTG 
CCAGATCCTC ACAGCTCTCC CAAAGCCAAA CCGGACCTGT CTGCTCTGAG AGCTCCGACC 
CGGGACGAGC CCATCCAGAG CAGCTACTTG CCCCCCAAGT CCACCATCAA TGGCATGGAT 
GCTCCTCCCA AAACACTCGG CGTGCCGACC AGCTACAACC GCTACGTCCC CAAGCCTTAC 
ACCAGCTCGG CGCGACCCTT CGAGCGCAAG TTCGAGAGTC CGAAATTCAA CCACAACCTG 
CTTCCCAACG ACACGCAGTC CAAACCCAGT GTCAACAACA ACCTGAAGCC TCAGATCTCC 
CCACAACCCC AGGACACGGA CAGCGGCGTC GACACCTTCA CTCGCACCAT GGACAACAGG 
CCAAAATATC AGCACAACAA CATCAACGCC ATTCCCAAAG CCGTCCCAGT CAGCCCCAGT 
GCACTGGATG ATGATGAAGA AGATGAGGGA CACACAGTTG TGGCCACGGC GCGGGGCATC 
TTTAACTGTA ATGGCGGGGT GTTGAGCTCT ATAGAGACGG GCGTCAGCAT CATTATCCCA 
CAGGGAGCCA TTCCTGATAG TGTGGAGCAG GAGATCTACT TCAAAGTGTG CCGGGACAAC 
AGCATCCTGC CTCCTCTGGA TAAGGAGAAA GGTGAAACTC TGCTGAGTCC GCTGGTGATG 
TGTGGTCCTC ACGGCCTGAA GTTCCTGAAG CCTGTGGAGC TGCGCTTGCC ACACTGTGCG 
TCTATGACCC CTGATGGTTG GTCTTTTGCT CTAAAATCCT CCGACTCCTC GTCGGGTGAC 
CCCAAATGCT GGCAAAACAA GTGTCTACCA GGAGACCCCA ATTACCTGGT GGGCGCAAAC 
TGTGTGTCTG TGCTGATCGA CCACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296407.1
CDS	115..6204
Translation	MLLSVFGGPVEEVLAGLLKLKMFGSGSKKWFIKKNKRQRTKVQRKPASGRRNKRVSRVLKRHKEVIVKQLDVSKVLPTLVYERVFTLGEYKEILGQDSSGKRAELFLDLLSQKEPAAFSAFCGVLEEVCPHLLTCFLLDGTDGSAGENKKQGQTKPAHSQQNALSKGQDTVCFPPMYTDPLFDTRVFPKHDKDKYGPAVYYPKFNAYIPNPDKVSVLDESEGSGHGKPSLRRIKGRIHRSKSLDSIDLLDSNSAAMDETVIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGLLQENDRVVMVNAVSMDNVDHAYAVQQLRKSGKNAKITIRRKRKVQIPSGRPGERETMSEHDEDDDSYEDEIYESRSARSGPSAYSGGGGGGGGGGASGRRSGRGDRLSGRRERDRERSGSRERSLSPRSDRHSVSSNLPPRPAKVTLVKSRKNEEYGLRLASHIFVKDISPESLAARDGNIQEGDVVLKINGTVTENLSLIDAKKLIERSKGKLKMVVQRDERATLLNIPDLDDSIPSANASDRDDISDIHSVASDHSNRSHDRKRSSRSRSPDRRSEPSDHSRHSPPQISNGRATGPTAQRTRPVHRSRDEDRISKAGPLPLPAKMVEEMPKEQSAARETEKQLPPLPEPKPVYAQPGQPDVDLPVSPADAPVPSVTHDDSILRPSMKLVKFKKGESVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFANIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHQEVERGIIPNKNRAEQLSSVQYTLPKTPGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVVIFGPIADVAREKLSREETDLFELAKSEPRDAGTDQRSSGIIRLHTIKQIIDRDKHAVLDITPNAVDRLNYAQWYPIVVFLNPDSKQGVKNMRTRLCPESRKSARKLYERALKLRKNNHHLFTTTINLNNMNDGWYGALKESIQQQQNQLVWVSEGKADGAPDDDLDIHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETMNDEVGLPSEPAITRSSEPVREDPPVIQDAAGYPSYQHAVPQPEAVSRIDPAGFKMAAPQQQAEAALASPPPPSAAVQPTATLAGTNPEEPPGTPQADSLNSPVPVPDPQPEPELAQPPTHDPHQSLPPGPDPKMYKKDLYSMDEPVRVTHGVKPPPPQQQQQPLGPPTSLSYSHQPVYQDQQPYRQYDHPPYGYDGGGYAQPKPHNYDPHLHYDNRVPHYNEQWPTYDQQQQQQTSPQPPPASGFPQGHQPPPPPPMGYEPRSPYEDGPNRDFSPPQSQYDGVSPMGYDHRPRHPKPAPVRYEEPPPPPPPVSYDARSPFEPEPHAFPGNTHRSPDPPKQYYGDAAMRPLYNPGPPNRVYKHEPVNSEPPVPPPKPESVLSPGEPPHSAASKPLPPPPRDDDDDDDDDDDPAMKPQSVLNRVKMFENKRSVSVDRAKDAPDVTGIRPTDLPKPASTPGPVLKANSLSNLEQDKPSYRAPEPQKPQTRVGDDVVRSNHYDPDDDEEYYRKQLSFFDRRSFDSKVMNPPSAGVNRFHEPPKPPQTQIAYPFARTESVEKVSPVDKRYEPLPPVNPSPAPYSQNTPVAPPTSLPKLTNIEVNSLPDPHSSPKAKPDLSALRAPTRDEPIQSSYLPPKSTINGMDAPPKTLGVPTSYNRYVPKPYTSSARPFERKFESPKFNHNLLPNDTQSKPSVNNNLKPQISPQPQDTDSGVDTFTRTMDNRPKYQHNNINAIPKAVPVSPSALDDDEEDEGHTVVATARGIFNCNGGVLSSIETGVSIIIPQGAIPDSVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKCWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_009298132.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tight junction protein 1b (tjp1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298132.2

ORF Insert Sequence:

ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
TCCCGCAAGA ATGAAGAATA CGGGCTTCGC CTGGCCAGCC ACATCTTCGT GAAGGACATT 
TCCCCCGAGA GCCTGGCAGC CCGAGACGGA AACATCCAAG AGGGAGACGT GGTGCTTAAG 
ATTAATGGCA CGGTAACAGA GAATTTGTCC CTGATCGATG CTAAGAAGCT GATCGAGAGG 
TCAAAGGGAA AGCTGAAGAT GGTGGTGCAG AGAGACGAGC GGGCGACGCT GCTGAACATC 
CCCGATCTGG ACGACAGCAT ACCTTCTGCT AATGCCTCGG ACAGAGATGA TATTTCAGAC 
ATCCACTCCG TGGCCTCTGA TCATTCCAAC CGATCCCACG ACCGGAAGAG GAGCAGCCGC 
TCTCGTTCTC CAGACCGCCG CTCCGAACCC TCAGACCACT CCAGACACTC TCCACCACAG 
ATCAGCAACG GCAGGGCCAC AGGGCCGACG GCTCAGAGAA CGCGGCCAGT TCACAGGAGC 
CGTGATGAAG ACAGGATCTC CAAGGCCGGG CCGCTTCCTC TACCTGCTAA GATGGTGGAG 
GAGATGCCGA AAGAGCAGAG CGCAGCCAGA GAAACAGAGA AACAGCTCCC CCCTCTGCCA 
GAGCCCAAAC CTGTGTACGC TCAGCCGGGC CAGCCGGACG TGGACCTGCC TGTTAGTCCG 
GCAGATGCTC CTGTGCCCAG CGTCACACAT GATGACAGCA TCTTACGGCC GAGCATGAAG 
CTGGTGAAGT TCAAGAAGGG CGAGAGCGTG GGCTTGAGGC TCGCTGGGGG GAACGACGTT 
GGGATATTTG TGGCGGGAGT TCTGGAGGAC AGTCCTGCTG CTAAAGAGGG CCTGGAAGAG 
GGAGACCAGA TTCTCAGGGT AAATAATGTG GATTTTGCAA ATATCATCCG AGAGGAGGCA 
GTGCTGTTCC TGCTGGATCT GCCCAAAGGA GAGGAGGTCA CCATCCTGGC CCAGAAGAAG 
AAAGACGTGT ACCGCCGCAT TGTAGAGTCT GACGTGGGAG ACTCGTTCTA CATCAGGACT 
CATTTTGAAT ATGAGAAGGA GTCGCCCTAT GGCCTGAGCT TCAATAAGGG GGAGGTCTTC 
AGAGTCGTCG ACACCCTTTA CAATGGCAAA CTGGGCTCCT GGCTCGCCAT CCGCATCGGC 
AAAAACCACC AGGAGGTGGA GCGGGGCATC ATCCCCAACA AGAACAGGGC TGAGCAGTTA 
TCAAGTGTCC AGTACACTCT CCCAAAGACT CCAGGAGGGG ATCGCGCCGA CTTCTGGAGA 
TTCAGAGGCC TCCGCAGCTC CAAACGAAAC CTGAGGAAGA GCAGAGAAGA CCTGTCTGCT 
CAGCCGGTCC AAACCAAATT CCCTGCTTAT GAAAGAGTGG TGCTTAGAGA AGCTGGTTTC 
TTGAGGCCAG TGGTCATTTT CGGACCGATC GCGGATGTGG CTAGAGAGAA GCTGTCCAGA 
GAGGAGACTG ACCTCTTCGA ACTTGCAAAG AGTGAACCCA GAGATGCGGG CACAGACCAG 
CGCAGCTCCG GAATCATCCG TCTCCATACC ATTAAACAGA TCATTGACCG AGACAAGCAC 
GCGGTGCTGG ACATCACCCC TAATGCGGTG GACCGTCTGA ACTACGCTCA GTGGTACCCC 
ATCGTGGTGT TTCTGAATCC AGACAGCAAA CAGGGCGTCA AGAACATGAG GACCAGGCTC 
TGTCCCGAGT CCAGGAAGAG CGCCCGAAAG CTCTACGAGC GTGCTCTCAA ACTGAGGAAG 
AACAACCATC ACCTTTTCAC CACCACTATT AACTTGAACA ACATGAATGA CGGGTGGTAC 
GGTGCTTTGA AAGAGAGCAT CCAGCAGCAG CAGAACCAGT TGGTCTGGGT CTCAGAGGGC 
AAGGCCGACG GCGCTCCTGA CGATGACCTT GACATCCATG ACGACCGCCT CTCCTACCTC 
TCGGCTCCGG GCAGCGAGTA CAGCATGTAC AGCACCGACA GCCGCCACAC GTCCGACTAT 
GAGGACACCG ACACGGAGGG TGGTGCCTAC ACCGACCAGG AGCTGGACGA GACCATGAAC 
GATGAGGTGG GCCTGCCTAG CGAGCCTGCC ATCACACGCT CATCTGAGCC TGTGCGAGAG 
GACCCGCCAG TCATTCAGGA CGCAGCTGGA TACCCCAGCT ACCAGCATGC GGTACCGCAA 
CCTGAAGCGG TGAGCCGCAT TGACCCGGCC GGGTTTAAGA TGGCAGCGCC GCAGCAGCAA 
GCTGAGGCTG CTCTGGCCTC GCCGCCCCCT CCCTCTGCAG CGGTCCAGCC CACCGCAACA 
CTAGCGGGTA CGAACCCCGA GGAGCCGCCT GGCACCCCTC AGGCCGACTC CCTTAACAGC 
CCCGTCCCTG TTCCCGACCC CCAGCCCGAA CCTGAGCTCG CTCAGCCCCC AACACACGAC 
CCCCACCAGT CGCTTCCTCC TGGCCCAGAT CCAAAGATGT ATAAAAAGGA TCTGTACAGC 
ATGGACGAGC CGGTGCGAGT GACCCACGGC GTGAAGCCTC CCCCTCCCCA GCAGCAGCAG 
CAGCCTTTAG GCCCCCCAAC CTCGCTCTCC TACAGCCACC AGCCTGTCTA CCAGGACCAA 
CAGCCATACC GCCAGTACGA CCACCCGCCT TATGGCTATG ACGGTGGCGG CTACGCACAA 
CCAAAGCCTC ACAACTACGA CCCGCACCTG CACTACGACA ACCGTGTGCC TCACTATAAC 
GAGCAGTGGC CCACGTACGA CCAGCAGCAG CAGCAGCAGA CTTCCCCCCA GCCGCCACCT 
GCGTCCGGCT TCCCTCAGGG CCACCAGCCT CCGCCTCCAC CACCGATGGG ATATGAGCCA 
CGCTCCCCAT ACGAGGACGG ACCTAACAGG GATTTCAGCC CTCCTCAGTC CCAGTATGAC 
GGTGTGTCCC CAATGGGCTA CGATCACCGG CCGCGGCACC CCAAACCTGC ACCGGTCCGA 
TATGAAGAGC CTCCACCTCC ACCTCCTCCG GTGTCCTACG ATGCACGGTC ACCTTTCGAA 
CCCGAGCCGC ATGCTTTCCC AGGCAACACA CATCGCTCGC CAGACCCACC GAAACAGTAT 
TATGGAGACG CCGCTATGAG GCCCTTGTAC AACCCTGGAC CTCCGAACAG AGTGTACAAA 
CATGAGCCGG TGAACTCTGA GCCTCCTGTA CCGCCTCCCA AACCTGAATC TGTCCTGTCT 
CCAGGAGAAC CGCCCCATTC TGCAGCTTCC AAACCATTGC CGCCTCCTCC CAGAGATGAT 
GACGATGATG ATGACGATGA TGATGACCCT GCCATGAAGC CCCAGTCTGT GCTCAACAGG 
GTCAAAATGT TCGAAAACAA ACGCTCTGTG TCGGTAGACC GAGCCAAGGA CGCACCTGAC 
GTCACTGGAA TTAGGCCCAC AGATCTCCCC AAACCTGCGA GCACCCCTGG ACCTGTGCTC 
AAAGCCAACT CTCTCAGCAA CCTGGAGCAG GACAAACCCT CCTACAGAGC TCCAGAGCCG 
CAGAAACCTC AGACGCGTGT TGGGGACGAT GTGGTTCGCT CCAACCACTA CGACCCTGAT 
GATGATGAAG AGTACTACAG AAAACAGCTC TCATTTTTCG ACCGCCGCAG TTTCGACAGT 
AAGGTCATGA ATCCACCCAG CGCTGGAGTA AACCGCTTCC ATGAGCCGCC CAAACCACCC 
CAGACACAGA TCGCTTATCC TTTCGCTAGG ACGGAGTCTG TGGAGAAGGT GAGTCCTGTG 
GACAAGAGAT ACGAGCCTCT GCCGCCAGTC AACCCTTCAC CTGCTCCATA CAGCCAGAAC 
ACACCTGTCG CTCCGCCCAC ATCCCTGCCC AAACTCACCA ACATCGAGGT GAACTCTCTG 
CCAGATCCTC ACAGCTCTCC CAAAGCCAAA CCGGACCTGT CTGCTCTGAG AGCTCCGACC 
CGGGACGAGC CCATCCAGAG CAGCTACTTG CCCCCCAAGT CCACCATCAA TGGCATGGAT 
GCTCCTCCCA AAACACTCGG CGTGCCGACC AGCTACAACC GCTACGTCCC CAAGCCTTAC 
ACCAGCTCGG CGCGACCCTT CGAGCGCAAG TTCGAGAGTC CGAAATTCAA CCACAACCTG 
CTTCCCAACG ACACGCAGTC CAAACCCAGT GTCAACAACA ACCTGAAGCC TCAGATCTCC 
CCACAACCCC AGGACACGGA CAGCGGCGTC GACACCTTCA CTCGCACCAT GGACAACAGG 
CCAAAATATC AGCACAACAA CATCAACGCC ATTCCCAAAG CCGTCCCAGT CAGCCCCAGT 
GCACTGGATG ATGATGAAGA AGATGAGGGA CACACAGTTG TGGCCACGGC GCGGGGCATC 
TTTAACTGTA ATGGCGGGGT GTTGAGCTCT ATAGAGACGG GCGTCAGCAT CATTATCCCA 
CAGGGAGCCA TTCCTGATAG TGTGGAGCAG GAGATCTACT TCAAAGTGTG CCGGGACAAC 
AGCATCCTGC CTCCTCTGGA TAAGGAGAAA GGTGAAACTC TGCTGAGTCC GCTGGTGATG 
TGTGGTCCTC ACGGCCTGAA GTTCCTGAAG CCTGTGGAGC TGCGCTTGCC ACACTGTGCG 
TCTATGACCC CTGATGGTTG GTCTTTTGCT CTAAAATCCT CCGACTCCTC GTCGGGTGAC 
CCCAAATGCT GGCAAAACAA GTGTCTACCA GGAGACCCCA ATTACCTGGT GGGCGCAAAC 
TGTGTGTCTG TGCTGATCGA CCACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp1b ( XM_009298132.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296407.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X8, mRNA.

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CloneID	ODa44622
Clone ID Related Accession (Same CDS sequence)	XM_009298132.2 , XM_009298132.3
Accession Version	XM_009298132.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6090bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tight junction protein ZO-1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009298132.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
TCCCGCAAGA ATGAAGAATA CGGGCTTCGC CTGGCCAGCC ACATCTTCGT GAAGGACATT 
TCCCCCGAGA GCCTGGCAGC CCGAGACGGA AACATCCAAG AGGGAGACGT GGTGCTTAAG 
ATTAATGGCA CGGTAACAGA GAATTTGTCC CTGATCGATG CTAAGAAGCT GATCGAGAGG 
TCAAAGGGAA AGCTGAAGAT GGTGGTGCAG AGAGACGAGC GGGCGACGCT GCTGAACATC 
CCCGATCTGG ACGACAGCAT ACCTTCTGCT AATGCCTCGG ACAGAGATGA TATTTCAGAC 
ATCCACTCCG TGGCCTCTGA TCATTCCAAC CGATCCCACG ACCGGAAGAG GAGCAGCCGC 
TCTCGTTCTC CAGACCGCCG CTCCGAACCC TCAGACCACT CCAGACACTC TCCACCACAG 
ATCAGCAACG GCAGGGCCAC AGGGCCGACG GCTCAGAGAA CGCGGCCAGT TCACAGGAGC 
CGTGATGAAG ACAGGATCTC CAAGGCCGGG CCGCTTCCTC TACCTGCTAA GATGGTGGAG 
GAGATGCCGA AAGAGCAGAG CGCAGCCAGA GAAACAGAGA AACAGCTCCC CCCTCTGCCA 
GAGCCCAAAC CTGTGTACGC TCAGCCGGGC CAGCCGGACG TGGACCTGCC TGTTAGTCCG 
GCAGATGCTC CTGTGCCCAG CGTCACACAT GATGACAGCA TCTTACGGCC GAGCATGAAG 
CTGGTGAAGT TCAAGAAGGG CGAGAGCGTG GGCTTGAGGC TCGCTGGGGG GAACGACGTT 
GGGATATTTG TGGCGGGAGT TCTGGAGGAC AGTCCTGCTG CTAAAGAGGG CCTGGAAGAG 
GGAGACCAGA TTCTCAGGGT AAATAATGTG GATTTTGCAA ATATCATCCG AGAGGAGGCA 
GTGCTGTTCC TGCTGGATCT GCCCAAAGGA GAGGAGGTCA CCATCCTGGC CCAGAAGAAG 
AAAGACGTGT ACCGCCGCAT TGTAGAGTCT GACGTGGGAG ACTCGTTCTA CATCAGGACT 
CATTTTGAAT ATGAGAAGGA GTCGCCCTAT GGCCTGAGCT TCAATAAGGG GGAGGTCTTC 
AGAGTCGTCG ACACCCTTTA CAATGGCAAA CTGGGCTCCT GGCTCGCCAT CCGCATCGGC 
AAAAACCACC AGGAGGTGGA GCGGGGCATC ATCCCCAACA AGAACAGGGC TGAGCAGTTA 
TCAAGTGTCC AGTACACTCT CCCAAAGACT CCAGGAGGGG ATCGCGCCGA CTTCTGGAGA 
TTCAGAGGCC TCCGCAGCTC CAAACGAAAC CTGAGGAAGA GCAGAGAAGA CCTGTCTGCT 
CAGCCGGTCC AAACCAAATT CCCTGCTTAT GAAAGAGTGG TGCTTAGAGA AGCTGGTTTC 
TTGAGGCCAG TGGTCATTTT CGGACCGATC GCGGATGTGG CTAGAGAGAA GCTGTCCAGA 
GAGGAGACTG ACCTCTTCGA ACTTGCAAAG AGTGAACCCA GAGATGCGGG CACAGACCAG 
CGCAGCTCCG GAATCATCCG TCTCCATACC ATTAAACAGA TCATTGACCG AGACAAGCAC 
GCGGTGCTGG ACATCACCCC TAATGCGGTG GACCGTCTGA ACTACGCTCA GTGGTACCCC 
ATCGTGGTGT TTCTGAATCC AGACAGCAAA CAGGGCGTCA AGAACATGAG GACCAGGCTC 
TGTCCCGAGT CCAGGAAGAG CGCCCGAAAG CTCTACGAGC GTGCTCTCAA ACTGAGGAAG 
AACAACCATC ACCTTTTCAC CACCACTATT AACTTGAACA ACATGAATGA CGGGTGGTAC 
GGTGCTTTGA AAGAGAGCAT CCAGCAGCAG CAGAACCAGT TGGTCTGGGT CTCAGAGGGC 
AAGGCCGACG GCGCTCCTGA CGATGACCTT GACATCCATG ACGACCGCCT CTCCTACCTC 
TCGGCTCCGG GCAGCGAGTA CAGCATGTAC AGCACCGACA GCCGCCACAC GTCCGACTAT 
GAGGACACCG ACACGGAGGG TGGTGCCTAC ACCGACCAGG AGCTGGACGA GACCATGAAC 
GATGAGGTGG GCCTGCCTAG CGAGCCTGCC ATCACACGCT CATCTGAGCC TGTGCGAGAG 
GACCCGCCAG TCATTCAGGA CGCAGCTGGA TACCCCAGCT ACCAGCATGC GGTACCGCAA 
CCTGAAGCGG TGAGCCGCAT TGACCCGGCC GGGTTTAAGA TGGCAGCGCC GCAGCAGCAA 
GCTGAGGCTG CTCTGGCCTC GCCGCCCCCT CCCTCTGCAG CGGTCCAGCC CACCGCAACA 
CTAGCGGGTA CGAACCCCGA GGAGCCGCCT GGCACCCCTC AGGCCGACTC CCTTAACAGC 
CCCGTCCCTG TTCCCGACCC CCAGCCCGAA CCTGAGCTCG CTCAGCCCCC AACACACGAC 
CCCCACCAGT CGCTTCCTCC TGGCCCAGAT CCAAAGATGT ATAAAAAGGA TCTGTACAGC 
ATGGACGAGC CGGTGCGAGT GACCCACGGC GTGAAGCCTC CCCCTCCCCA GCAGCAGCAG 
CAGCCTTTAG GCCCCCCAAC CTCGCTCTCC TACAGCCACC AGCCTGTCTA CCAGGACCAA 
CAGCCATACC GCCAGTACGA CCACCCGCCT TATGGCTATG ACGGTGGCGG CTACGCACAA 
CCAAAGCCTC ACAACTACGA CCCGCACCTG CACTACGACA ACCGTGTGCC TCACTATAAC 
GAGCAGTGGC CCACGTACGA CCAGCAGCAG CAGCAGCAGA CTTCCCCCCA GCCGCCACCT 
GCGTCCGGCT TCCCTCAGGG CCACCAGCCT CCGCCTCCAC CACCGATGGG ATATGAGCCA 
CGCTCCCCAT ACGAGGACGG ACCTAACAGG GATTTCAGCC CTCCTCAGTC CCAGTATGAC 
GGTGTGTCCC CAATGGGCTA CGATCACCGG CCGCGGCACC CCAAACCTGC ACCGGTCCGA 
TATGAAGAGC CTCCACCTCC ACCTCCTCCG GTGTCCTACG ATGCACGGTC ACCTTTCGAA 
CCCGAGCCGC ATGCTTTCCC AGGCAACACA CATCGCTCGC CAGACCCACC GAAACAGTAT 
TATGGAGACG CCGCTATGAG GCCCTTGTAC AACCCTGGAC CTCCGAACAG AGTGTACAAA 
CATGAGCCGG TGAACTCTGA GCCTCCTGTA CCGCCTCCCA AACCTGAATC TGTCCTGTCT 
CCAGGAGAAC CGCCCCATTC TGCAGCTTCC AAACCATTGC CGCCTCCTCC CAGAGATGAT 
GACGATGATG ATGACGATGA TGATGACCCT GCCATGAAGC CCCAGTCTGT GCTCAACAGG 
GTCAAAATGT TCGAAAACAA ACGCTCTGTG TCGGTAGACC GAGCCAAGGA CGCACCTGAC 
GTCACTGGAA TTAGGCCCAC AGATCTCCCC AAACCTGCGA GCACCCCTGG ACCTGTGCTC 
AAAGCCAACT CTCTCAGCAA CCTGGAGCAG GACAAACCCT CCTACAGAGC TCCAGAGCCG 
CAGAAACCTC AGACGCGTGT TGGGGACGAT GTGGTTCGCT CCAACCACTA CGACCCTGAT 
GATGATGAAG AGTACTACAG AAAACAGCTC TCATTTTTCG ACCGCCGCAG TTTCGACAGT 
AAGGTCATGA ATCCACCCAG CGCTGGAGTA AACCGCTTCC ATGAGCCGCC CAAACCACCC 
CAGACACAGA TCGCTTATCC TTTCGCTAGG ACGGAGTCTG TGGAGAAGGT GAGTCCTGTG 
GACAAGAGAT ACGAGCCTCT GCCGCCAGTC AACCCTTCAC CTGCTCCATA CAGCCAGAAC 
ACACCTGTCG CTCCGCCCAC ATCCCTGCCC AAACTCACCA ACATCGAGGT GAACTCTCTG 
CCAGATCCTC ACAGCTCTCC CAAAGCCAAA CCGGACCTGT CTGCTCTGAG AGCTCCGACC 
CGGGACGAGC CCATCCAGAG CAGCTACTTG CCCCCCAAGT CCACCATCAA TGGCATGGAT 
GCTCCTCCCA AAACACTCGG CGTGCCGACC AGCTACAACC GCTACGTCCC CAAGCCTTAC 
ACCAGCTCGG CGCGACCCTT CGAGCGCAAG TTCGAGAGTC CGAAATTCAA CCACAACCTG 
CTTCCCAACG ACACGCAGTC CAAACCCAGT GTCAACAACA ACCTGAAGCC TCAGATCTCC 
CCACAACCCC AGGACACGGA CAGCGGCGTC GACACCTTCA CTCGCACCAT GGACAACAGG 
CCAAAATATC AGCACAACAA CATCAACGCC ATTCCCAAAG CCGTCCCAGT CAGCCCCAGT 
GCACTGGATG ATGATGAAGA AGATGAGGGA CACACAGTTG TGGCCACGGC GCGGGGCATC 
TTTAACTGTA ATGGCGGGGT GTTGAGCTCT ATAGAGACGG GCGTCAGCAT CATTATCCCA 
CAGGGAGCCA TTCCTGATAG TGTGGAGCAG GAGATCTACT TCAAAGTGTG CCGGGACAAC 
AGCATCCTGC CTCCTCTGGA TAAGGAGAAA GGTGAAACTC TGCTGAGTCC GCTGGTGATG 
TGTGGTCCTC ACGGCCTGAA GTTCCTGAAG CCTGTGGAGC TGCGCTTGCC ACACTGTGCG 
TCTATGACCC CTGATGGTTG GTCTTTTGCT CTAAAATCCT CCGACTCCTC GTCGGGTGAC 
CCCAAATGCT GGCAAAACAA GTGTCTACCA GGAGACCCCA ATTACCTGGT GGGCGCAAAC 
TGTGTGTCTG TGCTGATCGA CCACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296407.1
CDS	110..6199
Translation	MLLSVFGGPVEEVLAGLLKLKMFGSGSKKWFIKKNKRQRTKVQRKPASGRRNKRVSRVLKRHKEVIVKQLDVSKVLPTLVYERVFTLGEYKEILGQDSSGKRAELFLDLLSQKEPAAFSAFCGVLEEVCPHLLTCFLLDGTDGSAGENKKQGQTKPAHSQQNALSKGQDTVCFPPMYTDPLFDTRVFPKHDKDKYGPAVYYPKFNAYIPNPDKVSVLDESEGSGHGKPSLRRIKGRIHRSKSLDSIDLLDSNSAAMDETVIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGLLQENDRVVMVNAVSMDNVDHAYAVQQLRKSGKNAKITIRRKRKVQIPSGRPGERETMSEHDEDDDSYEDEIYESRSARSGPSAYSGGGGGGGGGGASGRRSGRGDRLSGRRERDRERSGSRERSLSPRSDRHSVSSNLPPRPAKVTLVKSRKNEEYGLRLASHIFVKDISPESLAARDGNIQEGDVVLKINGTVTENLSLIDAKKLIERSKGKLKMVVQRDERATLLNIPDLDDSIPSANASDRDDISDIHSVASDHSNRSHDRKRSSRSRSPDRRSEPSDHSRHSPPQISNGRATGPTAQRTRPVHRSRDEDRISKAGPLPLPAKMVEEMPKEQSAARETEKQLPPLPEPKPVYAQPGQPDVDLPVSPADAPVPSVTHDDSILRPSMKLVKFKKGESVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFANIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHQEVERGIIPNKNRAEQLSSVQYTLPKTPGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVVIFGPIADVAREKLSREETDLFELAKSEPRDAGTDQRSSGIIRLHTIKQIIDRDKHAVLDITPNAVDRLNYAQWYPIVVFLNPDSKQGVKNMRTRLCPESRKSARKLYERALKLRKNNHHLFTTTINLNNMNDGWYGALKESIQQQQNQLVWVSEGKADGAPDDDLDIHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETMNDEVGLPSEPAITRSSEPVREDPPVIQDAAGYPSYQHAVPQPEAVSRIDPAGFKMAAPQQQAEAALASPPPPSAAVQPTATLAGTNPEEPPGTPQADSLNSPVPVPDPQPEPELAQPPTHDPHQSLPPGPDPKMYKKDLYSMDEPVRVTHGVKPPPPQQQQQPLGPPTSLSYSHQPVYQDQQPYRQYDHPPYGYDGGGYAQPKPHNYDPHLHYDNRVPHYNEQWPTYDQQQQQQTSPQPPPASGFPQGHQPPPPPPMGYEPRSPYEDGPNRDFSPPQSQYDGVSPMGYDHRPRHPKPAPVRYEEPPPPPPPVSYDARSPFEPEPHAFPGNTHRSPDPPKQYYGDAAMRPLYNPGPPNRVYKHEPVNSEPPVPPPKPESVLSPGEPPHSAASKPLPPPPRDDDDDDDDDDDPAMKPQSVLNRVKMFENKRSVSVDRAKDAPDVTGIRPTDLPKPASTPGPVLKANSLSNLEQDKPSYRAPEPQKPQTRVGDDVVRSNHYDPDDDEEYYRKQLSFFDRRSFDSKVMNPPSAGVNRFHEPPKPPQTQIAYPFARTESVEKVSPVDKRYEPLPPVNPSPAPYSQNTPVAPPTSLPKLTNIEVNSLPDPHSSPKAKPDLSALRAPTRDEPIQSSYLPPKSTINGMDAPPKTLGVPTSYNRYVPKPYTSSARPFERKFESPKFNHNLLPNDTQSKPSVNNNLKPQISPQPQDTDSGVDTFTRTMDNRPKYQHNNINAIPKAVPVSPSALDDDEEDEGHTVVATARGIFNCNGGVLSSIETGVSIIIPQGAIPDSVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKCWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_009298132.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tight junction protein 1b (tjp1b), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298132.3

ORF Insert Sequence:

ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
TCCCGCAAGA ATGAAGAATA CGGGCTTCGC CTGGCCAGCC ACATCTTCGT GAAGGACATT 
TCCCCCGAGA GCCTGGCAGC CCGAGACGGA AACATCCAAG AGGGAGACGT GGTGCTTAAG 
ATTAATGGCA CGGTAACAGA GAATTTGTCC CTGATCGATG CTAAGAAGCT GATCGAGAGG 
TCAAAGGGAA AGCTGAAGAT GGTGGTGCAG AGAGACGAGC GGGCGACGCT GCTGAACATC 
CCCGATCTGG ACGACAGCAT ACCTTCTGCT AATGCCTCGG ACAGAGATGA TATTTCAGAC 
ATCCACTCCG TGGCCTCTGA TCATTCCAAC CGATCCCACG ACCGGAAGAG GAGCAGCCGC 
TCTCGTTCTC CAGACCGCCG CTCCGAACCC TCAGACCACT CCAGACACTC TCCACCACAG 
ATCAGCAACG GCAGGGCCAC AGGGCCGACG GCTCAGAGAA CGCGGCCAGT TCACAGGAGC 
CGTGATGAAG ACAGGATCTC CAAGGCCGGG CCGCTTCCTC TACCTGCTAA GATGGTGGAG 
GAGATGCCGA AAGAGCAGAG CGCAGCCAGA GAAACAGAGA AACAGCTCCC CCCTCTGCCA 
GAGCCCAAAC CTGTGTACGC TCAGCCGGGC CAGCCGGACG TGGACCTGCC TGTTAGTCCG 
GCAGATGCTC CTGTGCCCAG CGTCACACAT GATGACAGCA TCTTACGGCC GAGCATGAAG 
CTGGTGAAGT TCAAGAAGGG CGAGAGCGTG GGCTTGAGGC TCGCTGGGGG GAACGACGTT 
GGGATATTTG TGGCGGGAGT TCTGGAGGAC AGTCCTGCTG CTAAAGAGGG CCTGGAAGAG 
GGAGACCAGA TTCTCAGGGT AAATAATGTG GATTTTGCAA ATATCATCCG AGAGGAGGCA 
GTGCTGTTCC TGCTGGATCT GCCCAAAGGA GAGGAGGTCA CCATCCTGGC CCAGAAGAAG 
AAAGACGTGT ACCGCCGCAT TGTAGAGTCT GACGTGGGAG ACTCGTTCTA CATCAGGACT 
CATTTTGAAT ATGAGAAGGA GTCGCCCTAT GGCCTGAGCT TCAATAAGGG GGAGGTCTTC 
AGAGTCGTCG ACACCCTTTA CAATGGCAAA CTGGGCTCCT GGCTCGCCAT CCGCATCGGC 
AAAAACCACC AGGAGGTGGA GCGGGGCATC ATCCCCAACA AGAACAGGGC TGAGCAGTTA 
TCAAGTGTCC AGTACACTCT CCCAAAGACT CCAGGAGGGG ATCGCGCCGA CTTCTGGAGA 
TTCAGAGGCC TCCGCAGCTC CAAACGAAAC CTGAGGAAGA GCAGAGAAGA CCTGTCTGCT 
CAGCCGGTCC AAACCAAATT CCCTGCTTAT GAAAGAGTGG TGCTTAGAGA AGCTGGTTTC 
TTGAGGCCAG TGGTCATTTT CGGACCGATC GCGGATGTGG CTAGAGAGAA GCTGTCCAGA 
GAGGAGACTG ACCTCTTCGA ACTTGCAAAG AGTGAACCCA GAGATGCGGG CACAGACCAG 
CGCAGCTCCG GAATCATCCG TCTCCATACC ATTAAACAGA TCATTGACCG AGACAAGCAC 
GCGGTGCTGG ACATCACCCC TAATGCGGTG GACCGTCTGA ACTACGCTCA GTGGTACCCC 
ATCGTGGTGT TTCTGAATCC AGACAGCAAA CAGGGCGTCA AGAACATGAG GACCAGGCTC 
TGTCCCGAGT CCAGGAAGAG CGCCCGAAAG CTCTACGAGC GTGCTCTCAA ACTGAGGAAG 
AACAACCATC ACCTTTTCAC CACCACTATT AACTTGAACA ACATGAATGA CGGGTGGTAC 
GGTGCTTTGA AAGAGAGCAT CCAGCAGCAG CAGAACCAGT TGGTCTGGGT CTCAGAGGGC 
AAGGCCGACG GCGCTCCTGA CGATGACCTT GACATCCATG ACGACCGCCT CTCCTACCTC 
TCGGCTCCGG GCAGCGAGTA CAGCATGTAC AGCACCGACA GCCGCCACAC GTCCGACTAT 
GAGGACACCG ACACGGAGGG TGGTGCCTAC ACCGACCAGG AGCTGGACGA GACCATGAAC 
GATGAGGTGG GCCTGCCTAG CGAGCCTGCC ATCACACGCT CATCTGAGCC TGTGCGAGAG 
GACCCGCCAG TCATTCAGGA CGCAGCTGGA TACCCCAGCT ACCAGCATGC GGTACCGCAA 
CCTGAAGCGG TGAGCCGCAT TGACCCGGCC GGGTTTAAGA TGGCAGCGCC GCAGCAGCAA 
GCTGAGGCTG CTCTGGCCTC GCCGCCCCCT CCCTCTGCAG CGGTCCAGCC CACCGCAACA 
CTAGCGGGTA CGAACCCCGA GGAGCCGCCT GGCACCCCTC AGGCCGACTC CCTTAACAGC 
CCCGTCCCTG TTCCCGACCC CCAGCCCGAA CCTGAGCTCG CTCAGCCCCC AACACACGAC 
CCCCACCAGT CGCTTCCTCC TGGCCCAGAT CCAAAGATGT ATAAAAAGGA TCTGTACAGC 
ATGGACGAGC CGGTGCGAGT GACCCACGGC GTGAAGCCTC CCCCTCCCCA GCAGCAGCAG 
CAGCCTTTAG GCCCCCCAAC CTCGCTCTCC TACAGCCACC AGCCTGTCTA CCAGGACCAA 
CAGCCATACC GCCAGTACGA CCACCCGCCT TATGGCTATG ACGGTGGCGG CTACGCACAA 
CCAAAGCCTC ACAACTACGA CCCGCACCTG CACTACGACA ACCGTGTGCC TCACTATAAC 
GAGCAGTGGC CCACGTACGA CCAGCAGCAG CAGCAGCAGA CTTCCCCCCA GCCGCCACCT 
GCGTCCGGCT TCCCTCAGGG CCACCAGCCT CCGCCTCCAC CACCGATGGG ATATGAGCCA 
CGCTCCCCAT ACGAGGACGG ACCTAACAGG GATTTCAGCC CTCCTCAGTC CCAGTATGAC 
GGTGTGTCCC CAATGGGCTA CGATCACCGG CCGCGGCACC CCAAACCTGC ACCGGTCCGA 
TATGAAGAGC CTCCACCTCC ACCTCCTCCG GTGTCCTACG ATGCACGGTC ACCTTTCGAA 
CCCGAGCCGC ATGCTTTCCC AGGCAACACA CATCGCTCGC CAGACCCACC GAAACAGTAT 
TATGGAGACG CCGCTATGAG GCCCTTGTAC AACCCTGGAC CTCCGAACAG AGTGTACAAA 
CATGAGCCGG TGAACTCTGA GCCTCCTGTA CCGCCTCCCA AACCTGAATC TGTCCTGTCT 
CCAGGAGAAC CGCCCCATTC TGCAGCTTCC AAACCATTGC CGCCTCCTCC CAGAGATGAT 
GACGATGATG ATGACGATGA TGATGACCCT GCCATGAAGC CCCAGTCTGT GCTCAACAGG 
GTCAAAATGT TCGAAAACAA ACGCTCTGTG TCGGTAGACC GAGCCAAGGA CGCACCTGAC 
GTCACTGGAA TTAGGCCCAC AGATCTCCCC AAACCTGCGA GCACCCCTGG ACCTGTGCTC 
AAAGCCAACT CTCTCAGCAA CCTGGAGCAG GACAAACCCT CCTACAGAGC TCCAGAGCCG 
CAGAAACCTC AGACGCGTGT TGGGGACGAT GTGGTTCGCT CCAACCACTA CGACCCTGAT 
GATGATGAAG AGTACTACAG AAAACAGCTC TCATTTTTCG ACCGCCGCAG TTTCGACAGT 
AAGGTCATGA ATCCACCCAG CGCTGGAGTA AACCGCTTCC ATGAGCCGCC CAAACCACCC 
CAGACACAGA TCGCTTATCC TTTCGCTAGG ACGGAGTCTG TGGAGAAGGT GAGTCCTGTG 
GACAAGAGAT ACGAGCCTCT GCCGCCAGTC AACCCTTCAC CTGCTCCATA CAGCCAGAAC 
ACACCTGTCG CTCCGCCCAC ATCCCTGCCC AAACTCACCA ACATCGAGGT GAACTCTCTG 
CCAGATCCTC ACAGCTCTCC CAAAGCCAAA CCGGACCTGT CTGCTCTGAG AGCTCCGACC 
CGGGACGAGC CCATCCAGAG CAGCTACTTG CCCCCCAAGT CCACCATCAA TGGCATGGAT 
GCTCCTCCCA AAACACTCGG CGTGCCGACC AGCTACAACC GCTACGTCCC CAAGCCTTAC 
ACCAGCTCGG CGCGACCCTT CGAGCGCAAG TTCGAGAGTC CGAAATTCAA CCACAACCTG 
CTTCCCAACG ACACGCAGTC CAAACCCAGT GTCAACAACA ACCTGAAGCC TCAGATCTCC 
CCACAACCCC AGGACACGGA CAGCGGCGTC GACACCTTCA CTCGCACCAT GGACAACAGG 
CCAAAATATC AGCACAACAA CATCAACGCC ATTCCCAAAG CCGTCCCAGT CAGCCCCAGT 
GCACTGGATG ATGATGAAGA AGATGAGGGA CACACAGTTG TGGCCACGGC GCGGGGCATC 
TTTAACTGTA ATGGCGGGGT GTTGAGCTCT ATAGAGACGG GCGTCAGCAT CATTATCCCA 
CAGGGAGCCA TTCCTGATAG TGTGGAGCAG GAGATCTACT TCAAAGTGTG CCGGGACAAC 
AGCATCCTGC CTCCTCTGGA TAAGGAGAAA GGTGAAACTC TGCTGAGTCC GCTGGTGATG 
TGTGGTCCTC ACGGCCTGAA GTTCCTGAAG CCTGTGGAGC TGCGCTTGCC ACACTGTGCG 
TCTATGACCC CTGATGGTTG GTCTTTTGCT CTAAAATCCT CCGACTCCTC GTCGGGTGAC 
CCCAAATGCT GGCAAAACAA GTGTCTACCA GGAGACCCCA ATTACCTGGT GGGCGCAAAC 
TGTGTGTCTG TGCTGATCGA CCACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp1b ( XM_021470730.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326405.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X9, mRNA.

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CloneID	ODa44623
Clone ID Related Accession (Same CDS sequence)	XM_021470730.1
Accession Version	XM_021470730.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6087bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tight junction protein ZO-1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
TCCCGCAAGA ATGAAGCATG TATGGCGTCA GGAAGTTCTC AGAAGGTGAC ATCAGCAGAA 
TACGGGCTTC GCCTGGCCAG CCACATCTTC GTGAAGGACA TTTCCCCCGA GAGCCTGGCA 
GCCCGAGACG GAAACATCCA AGAGGGAGAC GTGGTGCTTA AGAAGCTGAT CGAGAGGTCA 
AAGGGAAAGC TGAAGATGGT GGTGCAGAGA GACGAGCGGG CGACGCTGCT GAACATCCCC 
GATCTGGACG ACAGCATACC TTCTGCTAAT GCCTCGGACA GAGATGATAT TTCAGACATC 
CACTCCGTGG CCTCTGATCA TTCCAACCGA TCCCACGACC GGAAGAGGAG CAGCCGCTCT 
CGTTCTCCAG ACCGCCGCTC CGAACCCTCA GACCACTCCA GACACTCTCC ACCACAGATC 
AGCAACGGCA GGGCCACAGG GCCGACGGCT CAGAGAACGC GGCCAGTTCA CAGGAGCCGT 
GATGAAGACA GGATCTCCAA GGCCGGGCCG CTTCCTCTAC CTGCTAAGAT GGTGGAGGAG 
ATGCCGAAAG AGCAGAGCGC AGCCAGAGAA ACAGAGAAAC AGCTCCCCCC TCTGCCAGAG 
CCCAAACCTG TGTACGCTCA GCCGGGCCAG CCGGACGTGG ACCTGCCTGT TAGTCCGGCA 
GATGCTCCTG TGCCCAGCGT CACACATGAT GACAGCATCT TACGGCCGAG CATGAAGCTG 
GTGAAGTTCA AGAAGGGCGA GAGCGTGGGC TTGAGGCTCG CTGGGGGGAA CGACGTTGGG 
ATATTTGTGG CGGGAGTTCT GGAGGACAGT CCTGCTGCTA AAGAGGGCCT GGAAGAGGGA 
GACCAGATTC TCAGGGTAAA TAATGTGGAT TTTGCAAATA TCATCCGAGA GGAGGCAGTG 
CTGTTCCTGC TGGATCTGCC CAAAGGAGAG GAGGTCACCA TCCTGGCCCA GAAGAAGAAA 
GACGTGTACC GCCGCATTGT AGAGTCTGAC GTGGGAGACT CGTTCTACAT CAGGACTCAT 
TTTGAATATG AGAAGGAGTC GCCCTATGGC CTGAGCTTCA ATAAGGGGGA GGTCTTCAGA 
GTCGTCGACA CCCTTTACAA TGGCAAACTG GGCTCCTGGC TCGCCATCCG CATCGGCAAA 
AACCACCAGG AGGTGGAGCG GGGCATCATC CCCAACAAGA ACAGGGCTGA GCAGTTATCA 
AGTGTCCAGT ACACTCTCCC AAAGACTCCA GGAGGGGATC GCGCCGACTT CTGGAGATTC 
AGAGGCCTCC GCAGCTCCAA ACGAAACCTG AGGAAGAGCA GAGAAGACCT GTCTGCTCAG 
CCGGTCCAAA CCAAATTCCC TGCTTATGAA AGAGTGGTGC TTAGAGAAGC TGGTTTCTTG 
AGGCCAGTGG TCATTTTCGG ACCGATCGCG GATGTGGCTA GAGAGAAGCT GTCCAGAGAG 
GAGACTGACC TCTTCGAACT TGCAAAGAGT GAACCCAGAG ATGCGGGCAC AGACCAGCGC 
AGCTCCGGAA TCATCCGTCT CCATACCATT AAACAGATCA TTGACCGAGA CAAGCACGCG 
GTGCTGGACA TCACCCCTAA TGCGGTGGAC CGTCTGAACT ACGCTCAGTG GTACCCCATC 
GTGGTGTTTC TGAATCCAGA CAGCAAACAG GGCGTCAAGA ACATGAGGAC CAGGCTCTGT 
CCCGAGTCCA GGAAGAGCGC CCGAAAGCTC TACGAGCGTG CTCTCAAACT GAGGAAGAAC 
AACCATCACC TTTTCACCAC CACTATTAAC TTGAACAACA TGAATGACGG GTGGTACGGT 
GCTTTGAAAG AGAGCATCCA GCAGCAGCAG AACCAGTTGG TCTGGGTCTC AGAGGGCAAG 
GCCGACGGCG CTCCTGACGA TGACCTTGAC ATCCATGACG ACCGCCTCTC CTACCTCTCG 
GCTCCGGGCA GCGAGTACAG CATGTACAGC ACCGACAGCC GCCACACGTC CGACTATGAG 
GACACCGACA CGGAGGGTGG TGCCTACACC GACCAGGAGC TGGACGAGAC CATGAACGAT 
GAGGTGGGCC TGCCTAGCGA GCCTGCCATC ACACGCTCAT CTGAGCCTGT GCGAGAGGAC 
CCGCCAGTCA TTCAGGACGC AGCTGGATAC CCCAGCTACC AGCATGCGGT ACCGCAACCT 
GAAGCGGTGA GCCGCATTGA CCCGGCCGGG TTTAAGATGG CAGCGCCGCA GCAGCAAGCT 
GAGGCTGCTC TGGCCTCGCC GCCCCCTCCC TCTGCAGCGG TCCAGCCCAC CGCAACACTA 
GCGGGTACGA ACCCCGAGGA GCCGCCTGGC ACCCCTCAGG CCGACTCCCT TAACAGCCCC 
GTCCCTGTTC CCGACCCCCA GCCCGAACCT GAGCTCGCTC AGCCCCCAAC ACACGACCCC 
CACCAGTCGC TTCCTCCTGG CCCAGATCCA AAGATGTATA AAAAGGATCT GTACAGCATG 
GACGAGCCGG TGCGAGTGAC CCACGGCGTG AAGCCTCCCC CTCCCCAGCA GCAGCAGCAG 
CCTTTAGGCC CCCCAACCTC GCTCTCCTAC AGCCACCAGC CTGTCTACCA GGACCAACAG 
CCATACCGCC AGTACGACCA CCCGCCTTAT GGCTATGACG GTGGCGGCTA CGCACAACCA 
AAGCCTCACA ACTACGACCC GCACCTGCAC TACGACAACC GTGTGCCTCA CTATAACGAG 
CAGTGGCCCA CGTACGACCA GCAGCAGCAG CAGCAGACTT CCCCCCAGCC GCCACCTGCG 
TCCGGCTTCC CTCAGGGCCA CCAGCCTCCG CCTCCACCAC CGATGGGATA TGAGCCACGC 
TCCCCATACG AGGACGGACC TAACAGGGAT TTCAGCCCTC CTCAGTCCCA GTATGACGGT 
GTGTCCCCAA TGGGCTACGA TCACCGGCCG CGGCACCCCA AACCTGCACC GGTCCGATAT 
GAAGAGCCTC CACCTCCACC TCCTCCGGTG TCCTACGATG CACGGTCACC TTTCGAACCC 
GAGCCGCATG CTTTCCCAGG CAACACACAT CGCTCGCCAG ACCCACCGAA ACAGTATTAT 
GGAGACGCCG CTATGAGGCC CTTGTACAAC CCTGGACCTC CGAACAGAGT GTACAAACAT 
GAGCCGGTGA ACTCTGAGCC TCCTGTACCG CCTCCCAAAC CTGAATCTGT CCTGTCTCCA 
GGAGAACCGC CCCATTCTGC AGCTTCCAAA CCATTGCCGC CTCCTCCCAG AGATGATGAC 
GATGATGATG ACGATGATGA TGACCCTGCC ATGAAGCCCC AGTCTGTGCT CAACAGGGTC 
AAAATGTTCG AAAACAAACG CTCTGTGTCG GTAGACCGAG CCAAGGACGC ACCTGACGTC 
ACTGGAATTA GGCCCACAGA TCTCCCCAAA CCTGCGAGCA CCCCTGGACC TGTGCTCAAA 
GCCAACTCTC TCAGCAACCT GGAGCAGGAC AAACCCTCCT ACAGAGCTCC AGAGCCGCAG 
AAACCTCAGA CGCGTGTTGG GGACGATGTG GTTCGCTCCA ACCACTACGA CCCTGATGAT 
GATGAAGAGT ACTACAGAAA ACAGCTCTCA TTTTTCGACC GCCGCAGTTT CGACAGTAAG 
GTCATGAATC CACCCAGCGC TGGAGTAAAC CGCTTCCATG AGCCGCCCAA ACCACCCCAG 
ACACAGATCG CTTATCCTTT CGCTAGGACG GAGTCTGTGG AGAAGGTGAG TCCTGTGGAC 
AAGAGATACG AGCCTCTGCC GCCAGTCAAC CCTTCACCTG CTCCATACAG CCAGAACACA 
CCTGTCGCTC CGCCCACATC CCTGCCCAAA CTCACCAACA TCGAGGTGAA CTCTCTGCCA 
GATCCTCACA GCTCTCCCAA AGCCAAACCG GACCTGTCTG CTCTGAGAGC TCCGACCCGG 
GACGAGCCCA TCCAGAGCAG CTACTTGCCC CCCAAGTCCA CCATCAATGG CATGGATGCT 
CCTCCCAAAA CACTCGGCGT GCCGACCAGC TACAACCGCT ACGTCCCCAA GCCTTACACC 
AGCTCGGCGC GACCCTTCGA GCGCAAGTTC GAGAGTCCGA AATTCAACCA CAACCTGCTT 
CCCAACGACA CGCAGTCCAA ACCCAGTGTC AACAACAACC TGAAGCCTCA GATCTCCCCA 
CAACCCCAGG ACACGGACAG CGGCGTCGAC ACCTTCACTC GCACCATGGA CAACAGGCCA 
AAATATCAGC ACAACAACAT CAACGCCATT CCCAAAGCCG TCCCAGTCAG CCCCAGTGCA 
CTGGATGATG ATGAAGAAGA TGAGGGACAC ACAGTTGTGG CCACGGCGCG GGGCATCTTT 
AACTGTAATG GCGGGGTGTT GAGCTCTATA GAGACGGGCG TCAGCATCAT TATCCCACAG 
GGAGCCATTC CTGATAGTGT GGAGCAGGAG ATCTACTTCA AAGTGTGCCG GGACAACAGC 
ATCCTGCCTC CTCTGGATAA GGAGAAAGGT GAAACTCTGC TGAGTCCGCT GGTGATGTGT 
GGTCCTCACG GCCTGAAGTT CCTGAAGCCT GTGGAGCTGC GCTTGCCACA CTGTGCGTCT 
ATGACCCCTG ATGGTTGGTC TTTTGCTCTA AAATCCTCCG ACTCCTCGTC GGGTGACCCC 
AAATGCTGGC AAAACAAGTG TCTACCAGGA GACCCCAATT ACCTGGTGGG CGCAAACTGT 
GTGTCTGTGC TGATCGACCA CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326405.1
CDS	110..6196
Translation	MLLSVFGGPVEEVLAGLLKLKMFGSGSKKWFIKKNKRQRTKVQRKPASGRRNKRVSRVLKRHKEVIVKQLDVSKVLPTLVYERVFTLGEYKEILGQDSSGKRAELFLDLLSQKEPAAFSAFCGVLEEVCPHLLTCFLLDGTDGSAGENKKQGQTKPAHSQQNALSKGQDTVCFPPMYTDPLFDTRVFPKHDKDKYGPAVYYPKFNAYIPNPDKVSVLDESEGSGHGKPSLRRIKGRIHRSKSLDSIDLLDSNSAAMDETVIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGLLQENDRVVMVNAVSMDNVDHAYAVQQLRKSGKNAKITIRRKRKVQIPSGRPGERETMSEHDEDDDSYEDEIYESRSARSGPSAYSGGGGGGGGGGASGRRSGRGDRLSGRRERDRERSGSRERSLSPRSDRHSVSSNLPPRPAKVTLVKSRKNEACMASGSSQKVTSAEYGLRLASHIFVKDISPESLAARDGNIQEGDVVLKKLIERSKGKLKMVVQRDERATLLNIPDLDDSIPSANASDRDDISDIHSVASDHSNRSHDRKRSSRSRSPDRRSEPSDHSRHSPPQISNGRATGPTAQRTRPVHRSRDEDRISKAGPLPLPAKMVEEMPKEQSAARETEKQLPPLPEPKPVYAQPGQPDVDLPVSPADAPVPSVTHDDSILRPSMKLVKFKKGESVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFANIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHQEVERGIIPNKNRAEQLSSVQYTLPKTPGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVVIFGPIADVAREKLSREETDLFELAKSEPRDAGTDQRSSGIIRLHTIKQIIDRDKHAVLDITPNAVDRLNYAQWYPIVVFLNPDSKQGVKNMRTRLCPESRKSARKLYERALKLRKNNHHLFTTTINLNNMNDGWYGALKESIQQQQNQLVWVSEGKADGAPDDDLDIHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETMNDEVGLPSEPAITRSSEPVREDPPVIQDAAGYPSYQHAVPQPEAVSRIDPAGFKMAAPQQQAEAALASPPPPSAAVQPTATLAGTNPEEPPGTPQADSLNSPVPVPDPQPEPELAQPPTHDPHQSLPPGPDPKMYKKDLYSMDEPVRVTHGVKPPPPQQQQQPLGPPTSLSYSHQPVYQDQQPYRQYDHPPYGYDGGGYAQPKPHNYDPHLHYDNRVPHYNEQWPTYDQQQQQQTSPQPPPASGFPQGHQPPPPPPMGYEPRSPYEDGPNRDFSPPQSQYDGVSPMGYDHRPRHPKPAPVRYEEPPPPPPPVSYDARSPFEPEPHAFPGNTHRSPDPPKQYYGDAAMRPLYNPGPPNRVYKHEPVNSEPPVPPPKPESVLSPGEPPHSAASKPLPPPPRDDDDDDDDDDDPAMKPQSVLNRVKMFENKRSVSVDRAKDAPDVTGIRPTDLPKPASTPGPVLKANSLSNLEQDKPSYRAPEPQKPQTRVGDDVVRSNHYDPDDDEEYYRKQLSFFDRRSFDSKVMNPPSAGVNRFHEPPKPPQTQIAYPFARTESVEKVSPVDKRYEPLPPVNPSPAPYSQNTPVAPPTSLPKLTNIEVNSLPDPHSSPKAKPDLSALRAPTRDEPIQSSYLPPKSTINGMDAPPKTLGVPTSYNRYVPKPYTSSARPFERKFESPKFNHNLLPNDTQSKPSVNNNLKPQISPQPQDTDSGVDTFTRTMDNRPKYQHNNINAIPKAVPVSPSALDDDEEDEGHTVVATARGIFNCNGGVLSSIETGVSIIIPQGAIPDSVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKCWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_021470730.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tight junction protein 1b (tjp1b), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470730.1

ORF Insert Sequence:

ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
TCCCGCAAGA ATGAAGCATG TATGGCGTCA GGAAGTTCTC AGAAGGTGAC ATCAGCAGAA 
TACGGGCTTC GCCTGGCCAG CCACATCTTC GTGAAGGACA TTTCCCCCGA GAGCCTGGCA 
GCCCGAGACG GAAACATCCA AGAGGGAGAC GTGGTGCTTA AGAAGCTGAT CGAGAGGTCA 
AAGGGAAAGC TGAAGATGGT GGTGCAGAGA GACGAGCGGG CGACGCTGCT GAACATCCCC 
GATCTGGACG ACAGCATACC TTCTGCTAAT GCCTCGGACA GAGATGATAT TTCAGACATC 
CACTCCGTGG CCTCTGATCA TTCCAACCGA TCCCACGACC GGAAGAGGAG CAGCCGCTCT 
CGTTCTCCAG ACCGCCGCTC CGAACCCTCA GACCACTCCA GACACTCTCC ACCACAGATC 
AGCAACGGCA GGGCCACAGG GCCGACGGCT CAGAGAACGC GGCCAGTTCA CAGGAGCCGT 
GATGAAGACA GGATCTCCAA GGCCGGGCCG CTTCCTCTAC CTGCTAAGAT GGTGGAGGAG 
ATGCCGAAAG AGCAGAGCGC AGCCAGAGAA ACAGAGAAAC AGCTCCCCCC TCTGCCAGAG 
CCCAAACCTG TGTACGCTCA GCCGGGCCAG CCGGACGTGG ACCTGCCTGT TAGTCCGGCA 
GATGCTCCTG TGCCCAGCGT CACACATGAT GACAGCATCT TACGGCCGAG CATGAAGCTG 
GTGAAGTTCA AGAAGGGCGA GAGCGTGGGC TTGAGGCTCG CTGGGGGGAA CGACGTTGGG 
ATATTTGTGG CGGGAGTTCT GGAGGACAGT CCTGCTGCTA AAGAGGGCCT GGAAGAGGGA 
GACCAGATTC TCAGGGTAAA TAATGTGGAT TTTGCAAATA TCATCCGAGA GGAGGCAGTG 
CTGTTCCTGC TGGATCTGCC CAAAGGAGAG GAGGTCACCA TCCTGGCCCA GAAGAAGAAA 
GACGTGTACC GCCGCATTGT AGAGTCTGAC GTGGGAGACT CGTTCTACAT CAGGACTCAT 
TTTGAATATG AGAAGGAGTC GCCCTATGGC CTGAGCTTCA ATAAGGGGGA GGTCTTCAGA 
GTCGTCGACA CCCTTTACAA TGGCAAACTG GGCTCCTGGC TCGCCATCCG CATCGGCAAA 
AACCACCAGG AGGTGGAGCG GGGCATCATC CCCAACAAGA ACAGGGCTGA GCAGTTATCA 
AGTGTCCAGT ACACTCTCCC AAAGACTCCA GGAGGGGATC GCGCCGACTT CTGGAGATTC 
AGAGGCCTCC GCAGCTCCAA ACGAAACCTG AGGAAGAGCA GAGAAGACCT GTCTGCTCAG 
CCGGTCCAAA CCAAATTCCC TGCTTATGAA AGAGTGGTGC TTAGAGAAGC TGGTTTCTTG 
AGGCCAGTGG TCATTTTCGG ACCGATCGCG GATGTGGCTA GAGAGAAGCT GTCCAGAGAG 
GAGACTGACC TCTTCGAACT TGCAAAGAGT GAACCCAGAG ATGCGGGCAC AGACCAGCGC 
AGCTCCGGAA TCATCCGTCT CCATACCATT AAACAGATCA TTGACCGAGA CAAGCACGCG 
GTGCTGGACA TCACCCCTAA TGCGGTGGAC CGTCTGAACT ACGCTCAGTG GTACCCCATC 
GTGGTGTTTC TGAATCCAGA CAGCAAACAG GGCGTCAAGA ACATGAGGAC CAGGCTCTGT 
CCCGAGTCCA GGAAGAGCGC CCGAAAGCTC TACGAGCGTG CTCTCAAACT GAGGAAGAAC 
AACCATCACC TTTTCACCAC CACTATTAAC TTGAACAACA TGAATGACGG GTGGTACGGT 
GCTTTGAAAG AGAGCATCCA GCAGCAGCAG AACCAGTTGG TCTGGGTCTC AGAGGGCAAG 
GCCGACGGCG CTCCTGACGA TGACCTTGAC ATCCATGACG ACCGCCTCTC CTACCTCTCG 
GCTCCGGGCA GCGAGTACAG CATGTACAGC ACCGACAGCC GCCACACGTC CGACTATGAG 
GACACCGACA CGGAGGGTGG TGCCTACACC GACCAGGAGC TGGACGAGAC CATGAACGAT 
GAGGTGGGCC TGCCTAGCGA GCCTGCCATC ACACGCTCAT CTGAGCCTGT GCGAGAGGAC 
CCGCCAGTCA TTCAGGACGC AGCTGGATAC CCCAGCTACC AGCATGCGGT ACCGCAACCT 
GAAGCGGTGA GCCGCATTGA CCCGGCCGGG TTTAAGATGG CAGCGCCGCA GCAGCAAGCT 
GAGGCTGCTC TGGCCTCGCC GCCCCCTCCC TCTGCAGCGG TCCAGCCCAC CGCAACACTA 
GCGGGTACGA ACCCCGAGGA GCCGCCTGGC ACCCCTCAGG CCGACTCCCT TAACAGCCCC 
GTCCCTGTTC CCGACCCCCA GCCCGAACCT GAGCTCGCTC AGCCCCCAAC ACACGACCCC 
CACCAGTCGC TTCCTCCTGG CCCAGATCCA AAGATGTATA AAAAGGATCT GTACAGCATG 
GACGAGCCGG TGCGAGTGAC CCACGGCGTG AAGCCTCCCC CTCCCCAGCA GCAGCAGCAG 
CCTTTAGGCC CCCCAACCTC GCTCTCCTAC AGCCACCAGC CTGTCTACCA GGACCAACAG 
CCATACCGCC AGTACGACCA CCCGCCTTAT GGCTATGACG GTGGCGGCTA CGCACAACCA 
AAGCCTCACA ACTACGACCC GCACCTGCAC TACGACAACC GTGTGCCTCA CTATAACGAG 
CAGTGGCCCA CGTACGACCA GCAGCAGCAG CAGCAGACTT CCCCCCAGCC GCCACCTGCG 
TCCGGCTTCC CTCAGGGCCA CCAGCCTCCG CCTCCACCAC CGATGGGATA TGAGCCACGC 
TCCCCATACG AGGACGGACC TAACAGGGAT TTCAGCCCTC CTCAGTCCCA GTATGACGGT 
GTGTCCCCAA TGGGCTACGA TCACCGGCCG CGGCACCCCA AACCTGCACC GGTCCGATAT 
GAAGAGCCTC CACCTCCACC TCCTCCGGTG TCCTACGATG CACGGTCACC TTTCGAACCC 
GAGCCGCATG CTTTCCCAGG CAACACACAT CGCTCGCCAG ACCCACCGAA ACAGTATTAT 
GGAGACGCCG CTATGAGGCC CTTGTACAAC CCTGGACCTC CGAACAGAGT GTACAAACAT 
GAGCCGGTGA ACTCTGAGCC TCCTGTACCG CCTCCCAAAC CTGAATCTGT CCTGTCTCCA 
GGAGAACCGC CCCATTCTGC AGCTTCCAAA CCATTGCCGC CTCCTCCCAG AGATGATGAC 
GATGATGATG ACGATGATGA TGACCCTGCC ATGAAGCCCC AGTCTGTGCT CAACAGGGTC 
AAAATGTTCG AAAACAAACG CTCTGTGTCG GTAGACCGAG CCAAGGACGC ACCTGACGTC 
ACTGGAATTA GGCCCACAGA TCTCCCCAAA CCTGCGAGCA CCCCTGGACC TGTGCTCAAA 
GCCAACTCTC TCAGCAACCT GGAGCAGGAC AAACCCTCCT ACAGAGCTCC AGAGCCGCAG 
AAACCTCAGA CGCGTGTTGG GGACGATGTG GTTCGCTCCA ACCACTACGA CCCTGATGAT 
GATGAAGAGT ACTACAGAAA ACAGCTCTCA TTTTTCGACC GCCGCAGTTT CGACAGTAAG 
GTCATGAATC CACCCAGCGC TGGAGTAAAC CGCTTCCATG AGCCGCCCAA ACCACCCCAG 
ACACAGATCG CTTATCCTTT CGCTAGGACG GAGTCTGTGG AGAAGGTGAG TCCTGTGGAC 
AAGAGATACG AGCCTCTGCC GCCAGTCAAC CCTTCACCTG CTCCATACAG CCAGAACACA 
CCTGTCGCTC CGCCCACATC CCTGCCCAAA CTCACCAACA TCGAGGTGAA CTCTCTGCCA 
GATCCTCACA GCTCTCCCAA AGCCAAACCG GACCTGTCTG CTCTGAGAGC TCCGACCCGG 
GACGAGCCCA TCCAGAGCAG CTACTTGCCC CCCAAGTCCA CCATCAATGG CATGGATGCT 
CCTCCCAAAA CACTCGGCGT GCCGACCAGC TACAACCGCT ACGTCCCCAA GCCTTACACC 
AGCTCGGCGC GACCCTTCGA GCGCAAGTTC GAGAGTCCGA AATTCAACCA CAACCTGCTT 
CCCAACGACA CGCAGTCCAA ACCCAGTGTC AACAACAACC TGAAGCCTCA GATCTCCCCA 
CAACCCCAGG ACACGGACAG CGGCGTCGAC ACCTTCACTC GCACCATGGA CAACAGGCCA 
AAATATCAGC ACAACAACAT CAACGCCATT CCCAAAGCCG TCCCAGTCAG CCCCAGTGCA 
CTGGATGATG ATGAAGAAGA TGAGGGACAC ACAGTTGTGG CCACGGCGCG GGGCATCTTT 
AACTGTAATG GCGGGGTGTT GAGCTCTATA GAGACGGGCG TCAGCATCAT TATCCCACAG 
GGAGCCATTC CTGATAGTGT GGAGCAGGAG ATCTACTTCA AAGTGTGCCG GGACAACAGC 
ATCCTGCCTC CTCTGGATAA GGAGAAAGGT GAAACTCTGC TGAGTCCGCT GGTGATGTGT 
GGTCCTCACG GCCTGAAGTT CCTGAAGCCT GTGGAGCTGC GCTTGCCACA CTGTGCGTCT 
ATGACCCCTG ATGGTTGGTC TTTTGCTCTA AAATCCTCCG ACTCCTCGTC GGGTGACCCC 
AAATGCTGGC AAAACAAGTG TCTACCAGGA GACCCCAATT ACCTGGTGGG CGCAAACTGT 
GTGTCTGTGC TGATCGACCA CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp1b ( XM_021470732.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326407.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X11, mRNA.

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CloneID	ODa44624
Clone ID Related Accession (Same CDS sequence)	XM_021470732.1
Accession Version	XM_021470732.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6072bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tight junction protein ZO-1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
TCCCGCAAGA ATGAAGCATG TATGGCGTCA GGAAGTTCTC AGAAGGTGAC ATCAGCAGAA 
TACGGGCTTC GCCTGGCCAG CCACATCTTC GTGAAGGACA TTTCCCCCGA GAGCCTGGCA 
GCCCGAGACG GAAACATCCA AGAGGGAGAC GTGGTGCTTA AGATTAATGG CACGGTAACA 
GAGAATTTGT CCCTGATCGA TGCTAAGAAG CTGATCGAGA GGTCAAAGGG AAAGCTGAAG 
ATGGTGGTGC AGAGAGACGA GCGGGCGACG CTGCTGAACA TCCCCGATCT GGACGACAGC 
ATACCTTCTG CTAATGCCTC GGACAGAGAT GATATTTCAG ACATCCACTC CGTGGCCTCT 
GATCATTCCA ACCGATCCCA CGACCGGAAG AGGAGCAGCC GCTCTCGTTC TCCAGACCGC 
CGCTCCGAAC CCTCAGACCA CTCCAGACAC TCTCCACCAC AGATCAGCAA CGGCAGGGCC 
ACAGGGCCGA CGGCTCAGAG AACGCGGCCA GTTCACAGGA GCCGTGATGA AGACAGGATC 
TCCAAGGCCG GGCCGCTTCC TCTACCTGCT AAGATGGTGG AGGAGATGCC GAAAGAGCAG 
AGCGCAGCCA GAGAAACAGA GAAACAGCTC CCCCCTCTGC CAGAGCCCAA ACCTGTGTAC 
GCTCAGCCGG GCCAGCCGGA CGTGGACCTG CCTGTTAGTC CGGCAGATGC TCCTGTGCCC 
AGCGTCACAC ATGATGACAG CATCTTACGG CCGAGCATGA AGCTGGTGAA GTTCAAGAAG 
GGCGAGAGCG TGGGCTTGAG GCTCGCTGGG GGGAACGACG TTGGGATATT TGTGGCGGGA 
GTTCTGGAGG ACAGTCCTGC TGCTAAAGAG GGCCTGGAAG AGGGAGACCA GATTCTCAGG 
GTAAATAATG TGGATTTTGC AAATATCATC CGAGAGGAGG CAGTGCTGTT CCTGCTGGAT 
CTGCCCAAAG GAGAGGAGGT CACCATCCTG GCCCAGAAGA AGAAAGACGT GTACCGCCGC 
ATTGTAGAGT CTGACGTGGG AGACTCGTTC TACATCAGGA CTCATTTTGA ATATGAGAAG 
GAGTCGCCCT ATGGCCTGAG CTTCAATAAG GGGGAGGTCT TCAGAGTCGT CGACACCCTT 
TACAATGGCA AACTGGGCTC CTGGCTCGCC ATCCGCATCG GCAAAAACCA CCAGGAGGTG 
GAGCGGGGCA TCATCCCCAA CAAGAACAGG GCTGAGCAGT TATCAAGTGT CCAGTACACT 
CTCCCAAAGA CTCCAGGAGG GGATCGCGCC GACTTCTGGA GATTCAGAGG CCTCCGCAGC 
TCCAAACGAA ACCTGAGGAA GAGCAGAGAA GACCTGTCTG CTCAGCCGGT CCAAACCAAA 
TTCCCTGCTT ATGAAAGAGT GGTGCTTAGA GAAGCTGGTT TCTTGAGGCC AGTGGTCATT 
TTCGGACCGA TCGCGGATGT GGCTAGAGAG AAGCTGTCCA GAGAGGAGAC TGACCTCTTC 
GAACTTGCAA AGAGTGAACC CAGAGATGCG GGCACAGACC AGCGCAGCTC CGGAATCATC 
CGTCTCCATA CCATTAAACA GATCATTGAC CGAGACAAGC ACGCGGTGCT GGACATCACC 
CCTAATGCGG TGGACCGTCT GAACTACGCT CAGTGGTACC CCATCGTGGT GTTTCTGAAT 
CCAGACAGCA AACAGGGCGT CAAGAACATG AGGACCAGGC TCTGTCCCGA GTCCAGGAAG 
AGCGCCCGAA AGCTCTACGA GCGTGCTCTC AAACTGAGGA AGAACAACCA TCACCTTTTC 
ACCACCACTA TTAACTTGAA CAACATGAAT GACGGGTGGT ACGGTGCTTT GAAAGAGAGC 
ATCCAGCAGC AGCAGAACCA GTTGGTCTGG GTCTCAGAGG GCAAGGCCGA CGGCGCTCCT 
GACGATGACC TTGACATCCA TGACGACCGC CTCTCCTACC TCTCGGCTCC GGGCAGCGAG 
TACAGCATGT ACAGCACCGA CAGCCGCCAC ACGTCCGACT ATGAGGACAC CGACACGGAG 
GGTGGTGCCT ACACCGACCA GGAGCTGGAC GAGACCATGA ACGATGAGGT GGGCCTGCCT 
AGCGAGCCTG CCATCACACG CTCATCTGAG CCTGTGCGAG AGGACCCGCC AGTCATTCAG 
GACGCAGCTG GATACCCCAG CTACCAGCAT GCGGTACCGC AACCTGAAGC GGTGAGCCGC 
ATTGACCCGG CCGGGTTTAA GATGGCAGCG CCGCAGCAGC AAGCTGAGGC TGCTCTGGCC 
TCGCCGCCCC CTCCCTCTGC AGCGGTCCAG CCCACCGCAA CACTAGCGGG TACGAACCCC 
GAGGAGCCGC CTGGCACCCC TCAGGCCGAC TCCCTTAACA GCCCCGTCCC TGTTCCCGAC 
CCCCAGCCCG AACCTGAGCT CGCTCAGCCC CCAACACACG ACCCCCACCA GTCGCTTCCT 
CCTGGCCCAG ATCCAAAGAT GTATAAAAAG GATCTGTACA GCATGGACGA GCCGGTGCGA 
GTGACCCACG GCGTGAAGCC TCCCCCTCCC CAGCAGCAGC AGCAGCCTTT AGGCCCCCCA 
ACCTCGCTCT CCTACAGCCA CCAGCCTGTC TACCAGGACC AACAGCCATA CCGCCAGTAC 
GACCACCCGC CTTATGGCTA TGACGGTGGC GGCTACGCAC AACCAAAGCC TCACAACTAC 
GACCCGCACC TGCACTACGA CAACCGTGTG CCTCACTATA ACGAGCAGTG GCCCACGTAC 
GACCAGCAGC AGCAGCAGCA GACTTCCCCC CAGCCGCCAC CTGCGTCCGG CTTCCCTCAG 
GGCCACCAGC CTCCGCCTCC ACCACCGATG GGATATGAGC CACGCTCCCC ATACGAGGAC 
GGACCTAACA GGGATTTCAG CCCTCCTCAG TCCCAGTATG ACGGTGTGTC CCCAATGGGC 
TACGATCACC GGCCGCGGCA CCCCAAACCT GCACCGGTCC GATATGAAGA GCCTCCACCT 
CCACCTCCTC CGGTGTCCTA CGATGCACGG TCACCTTTCG AACCCGAGCC GCATGCTTTC 
CCAGGCAACA CACATCGCTC GCCAGACCCA CCGAAACAGT ATTATGGAGA CGCCGCTATG 
AGGCCCTTGT ACAACCCTGG ACCTCCGAAC AGAGTGTACA AACATGAGCC GGTGAACTCT 
GAGCCTCCTG TACCGCCTCC CAAACCTGAA TCTGTCCTGT CTCCAGGAGA ACCGCCCCAT 
TCTGCAGCTT CCAAACCATT GCCGCCTCCT CCCAGAGATG ATGACGATGA TGATGACGAT 
GATGATGACC CTGCCATGAA GCCCCAGTCT GTGCTCAACA GGGTCAAAAT GTTCGAAAAC 
AAACGCTCTG TGTCGGTAGA CCGAGCCAAG GACGCACCTG ACGTCACTGG AATTAGGCCC 
ACAGATCTCC CCAAACCTGC GAGCACCCCT GGACCTGTGC TCAAAGCCAA CTCTCTCAGC 
AACCTGGAGC AGGACAAACC CTCCTACAGA GCTCCAGAGC CGCAGAAACC TCAGACGCGT 
GTTGGGGACG ATGTGGTTCG CTCCAACCAC TACGACCCTG ATGATGATGA AGAGTACTAC 
AGAAAACAGC TCTCATTTTT CGACCGCCGC AGTTTCGACA GTAAGGTCAT GAATCCACCC 
AGCGCTGGAG TAAACCGCTT CCATGAGCCG CCCAAACCAC CCCAGACACA GATCGCTTAT 
CCTTTCGCTA GGACGGAGTC TGTGGAGAAG GTGAGTCCTG TGGACAAGAG ATACGAGCCT 
CTGCCGCCAG TCAACCCTTC ACCTGCTCCA TACAGCCAGA ACACACCTGT CGCTCCGCCC 
ACATCCCTGC CCAAACTCAC CAACATCGAG GTGAACTCTC TGCCAGATCC TCACAGCTCT 
CCCAAAGCCA AACCGGACCT GTCTGCTCTG AGAGCTCCGA CCCGGGACGA GCCCATCCAG 
AGCAGCTACT TGCCCCCCAA GTCCACCATC AATGGCATGG ATGCTCCTCC CAAAACACTC 
GGCGTGCCGA CCAGCTACAA CCGCTACGTC CCCAAGCCTT ACACCAGCTC GGCGCGACCC 
TTCGAGCGCA AGTTCGAGAG TCCGAAATTC AACCACAACC TGCTTCCCAA CGACACGCAG 
TCCAAACCCA GTGTCAACAA CAACCTGAAG CCTCAGATCT CCCCACAACC CCAGGACACG 
GACAGCGGCG TCGACACCTT CACTCGCACC ATGGACAACA GGCCAAAATA TCAGCACAAC 
AACATCAACG CCATTCCCAA AGCCGTCCCA GTCAGCCCCA GTGCACTGGA TGATGATGAA 
GAAGATGAGG GACACACAGT TGTGGCCACG GCGCGGGGCA TCTTTAACTG TAATGGCGGG 
GTGTTGAGCT CTATAGAGAC GGGCGTCAGC ATCATTATCC CACAGGGAGC CATTCCTGAT 
AGTGTGGAGC AGGAGATCTA CTTCAAAGTG TGCCGGGACA ACAGCATCCT GCCTCCTCTG 
GATAAGGAGA AAGGTGAAAC TCTGCTGAGT CCGCTGGTGA TGTGTGGTCC TCACGGCCTG 
AAGTTCCTGA AGCCTGTGGA GCTGCGCTTG CCACACTGTG ACCCCAAATG CTGGCAAAAC 
AAGTGTCTAC CAGGAGACCC CAATTACCTG GTGGGCGCAA ACTGTGTGTC TGTGCTGATC 
GACCACTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326407.1
CDS	110..6181
Translation	MLLSVFGGPVEEVLAGLLKLKMFGSGSKKWFIKKNKRQRTKVQRKPASGRRNKRVSRVLKRHKEVIVKQLDVSKVLPTLVYERVFTLGEYKEILGQDSSGKRAELFLDLLSQKEPAAFSAFCGVLEEVCPHLLTCFLLDGTDGSAGENKKQGQTKPAHSQQNALSKGQDTVCFPPMYTDPLFDTRVFPKHDKDKYGPAVYYPKFNAYIPNPDKVSVLDESEGSGHGKPSLRRIKGRIHRSKSLDSIDLLDSNSAAMDETVIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGLLQENDRVVMVNAVSMDNVDHAYAVQQLRKSGKNAKITIRRKRKVQIPSGRPGERETMSEHDEDDDSYEDEIYESRSARSGPSAYSGGGGGGGGGGASGRRSGRGDRLSGRRERDRERSGSRERSLSPRSDRHSVSSNLPPRPAKVTLVKSRKNEACMASGSSQKVTSAEYGLRLASHIFVKDISPESLAARDGNIQEGDVVLKINGTVTENLSLIDAKKLIERSKGKLKMVVQRDERATLLNIPDLDDSIPSANASDRDDISDIHSVASDHSNRSHDRKRSSRSRSPDRRSEPSDHSRHSPPQISNGRATGPTAQRTRPVHRSRDEDRISKAGPLPLPAKMVEEMPKEQSAARETEKQLPPLPEPKPVYAQPGQPDVDLPVSPADAPVPSVTHDDSILRPSMKLVKFKKGESVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFANIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHQEVERGIIPNKNRAEQLSSVQYTLPKTPGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVVIFGPIADVAREKLSREETDLFELAKSEPRDAGTDQRSSGIIRLHTIKQIIDRDKHAVLDITPNAVDRLNYAQWYPIVVFLNPDSKQGVKNMRTRLCPESRKSARKLYERALKLRKNNHHLFTTTINLNNMNDGWYGALKESIQQQQNQLVWVSEGKADGAPDDDLDIHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETMNDEVGLPSEPAITRSSEPVREDPPVIQDAAGYPSYQHAVPQPEAVSRIDPAGFKMAAPQQQAEAALASPPPPSAAVQPTATLAGTNPEEPPGTPQADSLNSPVPVPDPQPEPELAQPPTHDPHQSLPPGPDPKMYKKDLYSMDEPVRVTHGVKPPPPQQQQQPLGPPTSLSYSHQPVYQDQQPYRQYDHPPYGYDGGGYAQPKPHNYDPHLHYDNRVPHYNEQWPTYDQQQQQQTSPQPPPASGFPQGHQPPPPPPMGYEPRSPYEDGPNRDFSPPQSQYDGVSPMGYDHRPRHPKPAPVRYEEPPPPPPPVSYDARSPFEPEPHAFPGNTHRSPDPPKQYYGDAAMRPLYNPGPPNRVYKHEPVNSEPPVPPPKPESVLSPGEPPHSAASKPLPPPPRDDDDDDDDDDDPAMKPQSVLNRVKMFENKRSVSVDRAKDAPDVTGIRPTDLPKPASTPGPVLKANSLSNLEQDKPSYRAPEPQKPQTRVGDDVVRSNHYDPDDDEEYYRKQLSFFDRRSFDSKVMNPPSAGVNRFHEPPKPPQTQIAYPFARTESVEKVSPVDKRYEPLPPVNPSPAPYSQNTPVAPPTSLPKLTNIEVNSLPDPHSSPKAKPDLSALRAPTRDEPIQSSYLPPKSTINGMDAPPKTLGVPTSYNRYVPKPYTSSARPFERKFESPKFNHNLLPNDTQSKPSVNNNLKPQISPQPQDTDSGVDTFTRTMDNRPKYQHNNINAIPKAVPVSPSALDDDEEDEGHTVVATARGIFNCNGGVLSSIETGVSIIIPQGAIPDSVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCDPKCWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_021470732.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tight junction protein 1b (tjp1b), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470732.1

ORF Insert Sequence:

ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
TCCCGCAAGA ATGAAGCATG TATGGCGTCA GGAAGTTCTC AGAAGGTGAC ATCAGCAGAA 
TACGGGCTTC GCCTGGCCAG CCACATCTTC GTGAAGGACA TTTCCCCCGA GAGCCTGGCA 
GCCCGAGACG GAAACATCCA AGAGGGAGAC GTGGTGCTTA AGATTAATGG CACGGTAACA 
GAGAATTTGT CCCTGATCGA TGCTAAGAAG CTGATCGAGA GGTCAAAGGG AAAGCTGAAG 
ATGGTGGTGC AGAGAGACGA GCGGGCGACG CTGCTGAACA TCCCCGATCT GGACGACAGC 
ATACCTTCTG CTAATGCCTC GGACAGAGAT GATATTTCAG ACATCCACTC CGTGGCCTCT 
GATCATTCCA ACCGATCCCA CGACCGGAAG AGGAGCAGCC GCTCTCGTTC TCCAGACCGC 
CGCTCCGAAC CCTCAGACCA CTCCAGACAC TCTCCACCAC AGATCAGCAA CGGCAGGGCC 
ACAGGGCCGA CGGCTCAGAG AACGCGGCCA GTTCACAGGA GCCGTGATGA AGACAGGATC 
TCCAAGGCCG GGCCGCTTCC TCTACCTGCT AAGATGGTGG AGGAGATGCC GAAAGAGCAG 
AGCGCAGCCA GAGAAACAGA GAAACAGCTC CCCCCTCTGC CAGAGCCCAA ACCTGTGTAC 
GCTCAGCCGG GCCAGCCGGA CGTGGACCTG CCTGTTAGTC CGGCAGATGC TCCTGTGCCC 
AGCGTCACAC ATGATGACAG CATCTTACGG CCGAGCATGA AGCTGGTGAA GTTCAAGAAG 
GGCGAGAGCG TGGGCTTGAG GCTCGCTGGG GGGAACGACG TTGGGATATT TGTGGCGGGA 
GTTCTGGAGG ACAGTCCTGC TGCTAAAGAG GGCCTGGAAG AGGGAGACCA GATTCTCAGG 
GTAAATAATG TGGATTTTGC AAATATCATC CGAGAGGAGG CAGTGCTGTT CCTGCTGGAT 
CTGCCCAAAG GAGAGGAGGT CACCATCCTG GCCCAGAAGA AGAAAGACGT GTACCGCCGC 
ATTGTAGAGT CTGACGTGGG AGACTCGTTC TACATCAGGA CTCATTTTGA ATATGAGAAG 
GAGTCGCCCT ATGGCCTGAG CTTCAATAAG GGGGAGGTCT TCAGAGTCGT CGACACCCTT 
TACAATGGCA AACTGGGCTC CTGGCTCGCC ATCCGCATCG GCAAAAACCA CCAGGAGGTG 
GAGCGGGGCA TCATCCCCAA CAAGAACAGG GCTGAGCAGT TATCAAGTGT CCAGTACACT 
CTCCCAAAGA CTCCAGGAGG GGATCGCGCC GACTTCTGGA GATTCAGAGG CCTCCGCAGC 
TCCAAACGAA ACCTGAGGAA GAGCAGAGAA GACCTGTCTG CTCAGCCGGT CCAAACCAAA 
TTCCCTGCTT ATGAAAGAGT GGTGCTTAGA GAAGCTGGTT TCTTGAGGCC AGTGGTCATT 
TTCGGACCGA TCGCGGATGT GGCTAGAGAG AAGCTGTCCA GAGAGGAGAC TGACCTCTTC 
GAACTTGCAA AGAGTGAACC CAGAGATGCG GGCACAGACC AGCGCAGCTC CGGAATCATC 
CGTCTCCATA CCATTAAACA GATCATTGAC CGAGACAAGC ACGCGGTGCT GGACATCACC 
CCTAATGCGG TGGACCGTCT GAACTACGCT CAGTGGTACC CCATCGTGGT GTTTCTGAAT 
CCAGACAGCA AACAGGGCGT CAAGAACATG AGGACCAGGC TCTGTCCCGA GTCCAGGAAG 
AGCGCCCGAA AGCTCTACGA GCGTGCTCTC AAACTGAGGA AGAACAACCA TCACCTTTTC 
ACCACCACTA TTAACTTGAA CAACATGAAT GACGGGTGGT ACGGTGCTTT GAAAGAGAGC 
ATCCAGCAGC AGCAGAACCA GTTGGTCTGG GTCTCAGAGG GCAAGGCCGA CGGCGCTCCT 
GACGATGACC TTGACATCCA TGACGACCGC CTCTCCTACC TCTCGGCTCC GGGCAGCGAG 
TACAGCATGT ACAGCACCGA CAGCCGCCAC ACGTCCGACT ATGAGGACAC CGACACGGAG 
GGTGGTGCCT ACACCGACCA GGAGCTGGAC GAGACCATGA ACGATGAGGT GGGCCTGCCT 
AGCGAGCCTG CCATCACACG CTCATCTGAG CCTGTGCGAG AGGACCCGCC AGTCATTCAG 
GACGCAGCTG GATACCCCAG CTACCAGCAT GCGGTACCGC AACCTGAAGC GGTGAGCCGC 
ATTGACCCGG CCGGGTTTAA GATGGCAGCG CCGCAGCAGC AAGCTGAGGC TGCTCTGGCC 
TCGCCGCCCC CTCCCTCTGC AGCGGTCCAG CCCACCGCAA CACTAGCGGG TACGAACCCC 
GAGGAGCCGC CTGGCACCCC TCAGGCCGAC TCCCTTAACA GCCCCGTCCC TGTTCCCGAC 
CCCCAGCCCG AACCTGAGCT CGCTCAGCCC CCAACACACG ACCCCCACCA GTCGCTTCCT 
CCTGGCCCAG ATCCAAAGAT GTATAAAAAG GATCTGTACA GCATGGACGA GCCGGTGCGA 
GTGACCCACG GCGTGAAGCC TCCCCCTCCC CAGCAGCAGC AGCAGCCTTT AGGCCCCCCA 
ACCTCGCTCT CCTACAGCCA CCAGCCTGTC TACCAGGACC AACAGCCATA CCGCCAGTAC 
GACCACCCGC CTTATGGCTA TGACGGTGGC GGCTACGCAC AACCAAAGCC TCACAACTAC 
GACCCGCACC TGCACTACGA CAACCGTGTG CCTCACTATA ACGAGCAGTG GCCCACGTAC 
GACCAGCAGC AGCAGCAGCA GACTTCCCCC CAGCCGCCAC CTGCGTCCGG CTTCCCTCAG 
GGCCACCAGC CTCCGCCTCC ACCACCGATG GGATATGAGC CACGCTCCCC ATACGAGGAC 
GGACCTAACA GGGATTTCAG CCCTCCTCAG TCCCAGTATG ACGGTGTGTC CCCAATGGGC 
TACGATCACC GGCCGCGGCA CCCCAAACCT GCACCGGTCC GATATGAAGA GCCTCCACCT 
CCACCTCCTC CGGTGTCCTA CGATGCACGG TCACCTTTCG AACCCGAGCC GCATGCTTTC 
CCAGGCAACA CACATCGCTC GCCAGACCCA CCGAAACAGT ATTATGGAGA CGCCGCTATG 
AGGCCCTTGT ACAACCCTGG ACCTCCGAAC AGAGTGTACA AACATGAGCC GGTGAACTCT 
GAGCCTCCTG TACCGCCTCC CAAACCTGAA TCTGTCCTGT CTCCAGGAGA ACCGCCCCAT 
TCTGCAGCTT CCAAACCATT GCCGCCTCCT CCCAGAGATG ATGACGATGA TGATGACGAT 
GATGATGACC CTGCCATGAA GCCCCAGTCT GTGCTCAACA GGGTCAAAAT GTTCGAAAAC 
AAACGCTCTG TGTCGGTAGA CCGAGCCAAG GACGCACCTG ACGTCACTGG AATTAGGCCC 
ACAGATCTCC CCAAACCTGC GAGCACCCCT GGACCTGTGC TCAAAGCCAA CTCTCTCAGC 
AACCTGGAGC AGGACAAACC CTCCTACAGA GCTCCAGAGC CGCAGAAACC TCAGACGCGT 
GTTGGGGACG ATGTGGTTCG CTCCAACCAC TACGACCCTG ATGATGATGA AGAGTACTAC 
AGAAAACAGC TCTCATTTTT CGACCGCCGC AGTTTCGACA GTAAGGTCAT GAATCCACCC 
AGCGCTGGAG TAAACCGCTT CCATGAGCCG CCCAAACCAC CCCAGACACA GATCGCTTAT 
CCTTTCGCTA GGACGGAGTC TGTGGAGAAG GTGAGTCCTG TGGACAAGAG ATACGAGCCT 
CTGCCGCCAG TCAACCCTTC ACCTGCTCCA TACAGCCAGA ACACACCTGT CGCTCCGCCC 
ACATCCCTGC CCAAACTCAC CAACATCGAG GTGAACTCTC TGCCAGATCC TCACAGCTCT 
CCCAAAGCCA AACCGGACCT GTCTGCTCTG AGAGCTCCGA CCCGGGACGA GCCCATCCAG 
AGCAGCTACT TGCCCCCCAA GTCCACCATC AATGGCATGG ATGCTCCTCC CAAAACACTC 
GGCGTGCCGA CCAGCTACAA CCGCTACGTC CCCAAGCCTT ACACCAGCTC GGCGCGACCC 
TTCGAGCGCA AGTTCGAGAG TCCGAAATTC AACCACAACC TGCTTCCCAA CGACACGCAG 
TCCAAACCCA GTGTCAACAA CAACCTGAAG CCTCAGATCT CCCCACAACC CCAGGACACG 
GACAGCGGCG TCGACACCTT CACTCGCACC ATGGACAACA GGCCAAAATA TCAGCACAAC 
AACATCAACG CCATTCCCAA AGCCGTCCCA GTCAGCCCCA GTGCACTGGA TGATGATGAA 
GAAGATGAGG GACACACAGT TGTGGCCACG GCGCGGGGCA TCTTTAACTG TAATGGCGGG 
GTGTTGAGCT CTATAGAGAC GGGCGTCAGC ATCATTATCC CACAGGGAGC CATTCCTGAT 
AGTGTGGAGC AGGAGATCTA CTTCAAAGTG TGCCGGGACA ACAGCATCCT GCCTCCTCTG 
GATAAGGAGA AAGGTGAAAC TCTGCTGAGT CCGCTGGTGA TGTGTGGTCC TCACGGCCTG 
AAGTTCCTGA AGCCTGTGGA GCTGCGCTTG CCACACTGTG ACCCCAAATG CTGGCAAAAC 
AAGTGTCTAC CAGGAGACCC CAATTACCTG GTGGGCGCAA ACTGTGTGTC TGTGCTGATC 
GACCACTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp1b ( XM_009298135.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296410.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X13, mRNA.

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CloneID	ODa44625
Clone ID Related Accession (Same CDS sequence)	XM_009298135.3 , XM_009298135.2
Accession Version	XM_009298135.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tight junction protein ZO-1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009298135.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
TCCCGCAAGA ATGAAGAATA CGGGCTTCGC CTGGCCAGCC ACATCTTCGT GAAGGACATT 
TCCCCCGAGA GCCTGGCAGC CCGAGACGGA AACATCCAAG AGGGAGACGT GGTGCTTAAG 
ATTAATGGCA CGGTAACAGA GAATTTGTCC CTGATCGATG CTAAGAAGCT GATCGAGAGG 
TCAAAGGGAA AGCTGAAGAT GGTGGTGCAG AGAGACGAGC GGGCGACGCT GCTGAACATC 
CCCGATCTGG ACGACAGCAT ACCTTCTGCT AATGCCTCGG ACAGAGATGA TATTTCAGAC 
ATCCACTCCG TGGCCTCTGA TCATTCCAAC CGATCCCACG ACCGGAAGAG GAGCAGCCGC 
TCTCGTTCTC CAGACCGCCG CTCCGAACCC TCAGACCACT CCAGACACTC TCCACCACAG 
ATCAGCAACG GCAGTCACAG GAGCCGTGAT GAAGACAGGA TCTCCAAGGC CGGGCCGCTT 
CCTCTACCTG CTAAGATGGT GGAGGAGATG CCGAAAGAGC AGAGCGCAGC CAGAGAAACA 
GAGAAACAGC TCCCCCCTCT GCCAGAGCCC AAACCTGTGT ACGCTCAGCC GGGCCAGCCG 
GACGTGGACC TGCCTGTTAG TCCGGCAGAT GCTCCTGTGC CCAGCGTCAC ACATGATGAC 
AGCATCTTAC GGCCGAGCAT GAAGCTGGTG AAGTTCAAGA AGGGCGAGAG CGTGGGCTTG 
AGGCTCGCTG GGGGGAACGA CGTTGGGATA TTTGTGGCGG GAGTTCTGGA GGACAGTCCT 
GCTGCTAAAG AGGGCCTGGA AGAGGGAGAC CAGATTCTCA GGGTAAATAA TGTGGATTTT 
GCAAATATCA TCCGAGAGGA GGCAGTGCTG TTCCTGCTGG ATCTGCCCAA AGGAGAGGAG 
GTCACCATCC TGGCCCAGAA GAAGAAAGAC GTGTACCGCC GCATTGTAGA GTCTGACGTG 
GGAGACTCGT TCTACATCAG GACTCATTTT GAATATGAGA AGGAGTCGCC CTATGGCCTG 
AGCTTCAATA AGGGGGAGGT CTTCAGAGTC GTCGACACCC TTTACAATGG CAAACTGGGC 
TCCTGGCTCG CCATCCGCAT CGGCAAAAAC CACCAGGAGG TGGAGCGGGG CATCATCCCC 
AACAAGAACA GGGCTGAGCA GTTATCAAGT GTCCAGTACA CTCTCCCAAA GACTCCAGGA 
GGGGATCGCG CCGACTTCTG GAGATTCAGA GGCCTCCGCA GCTCCAAACG AAACCTGAGG 
AAGAGCAGAG AAGACCTGTC TGCTCAGCCG GTCCAAACCA AATTCCCTGC TTATGAAAGA 
GTGGTGCTTA GAGAAGCTGG TTTCTTGAGG CCAGTGGTCA TTTTCGGACC GATCGCGGAT 
GTGGCTAGAG AGAAGCTGTC CAGAGAGGAG ACTGACCTCT TCGAACTTGC AAAGAGTGAA 
CCCAGAGATG CGGGCACAGA CCAGCGCAGC TCCGGAATCA TCCGTCTCCA TACCATTAAA 
CAGATCATTG ACCGAGACAA GCACGCGGTG CTGGACATCA CCCCTAATGC GGTGGACCGT 
CTGAACTACG CTCAGTGGTA CCCCATCGTG GTGTTTCTGA ATCCAGACAG CAAACAGGGC 
GTCAAGAACA TGAGGACCAG GCTCTGTCCC GAGTCCAGGA AGAGCGCCCG AAAGCTCTAC 
GAGCGTGCTC TCAAACTGAG GAAGAACAAC CATCACCTTT TCACCACCAC TATTAACTTG 
AACAACATGA ATGACGGGTG GTACGGTGCT TTGAAAGAGA GCATCCAGCA GCAGCAGAAC 
CAGTTGGTCT GGGTCTCAGA GGGCAAGGCC GACGGCGCTC CTGACGATGA CCTTGACATC 
CATGACGACC GCCTCTCCTA CCTCTCGGCT CCGGGCAGCG AGTACAGCAT GTACAGCACC 
GACAGCCGCC ACACGTCCGA CTATGAGGAC ACCGACACGG AGGGTGGTGC CTACACCGAC 
CAGGAGCTGG ACGAGACCAT GAACGATGAG GTGGGCCTGC CTAGCGAGCC TGCCATCACA 
CGCTCATCTG AGCCTGTGCG AGAGGACCCG CCAGTCATTC AGGACGCAGC TGGATACCCC 
AGCTACCAGC ATGCGGTACC GCAACCTGAA GCGGTGAGCC GCATTGACCC GGCCGGGTTT 
AAGATGGCAG CGCCGCAGCA GCAAGCTGAG GCTGCTCTGG CCTCGCCGCC CCCTCCCTCT 
GCAGCGGTCC AGCCCACCGC AACACTAGCG GGTACGAACC CCGAGGAGCC GCCTGGCACC 
CCTCAGGCCG ACTCCCTTAA CAGCCCCGTC CCTGTTCCCG ACCCCCAGCC CGAACCTGAG 
CTCGCTCAGC CCCCAACACA CGACCCCCAC CAGTCGCTTC CTCCTGGCCC AGATCCAAAG 
ATGTATAAAA AGGATCTGTA CAGCATGGAC GAGCCGGTGC GAGTGACCCA CGGCGTGAAG 
CCTCCCCCTC CCCAGCAGCA GCAGCAGCCT TTAGGCCCCC CAACCTCGCT CTCCTACAGC 
CACCAGCCTG TCTACCAGGA CCAACAGCCA TACCGCCAGT ACGACCACCC GCCTTATGGC 
TATGACGGTG GCGGCTACGC ACAACCAAAG CCTCACAACT ACGACCCGCA CCTGCACTAC 
GACAACCGTG TGCCTCACTA TAACGAGCAG TGGCCCACGT ACGACCAGCA GCAGCAGCAG 
CAGACTTCCC CCCAGCCGCC ACCTGCGTCC GGCTTCCCTC AGGGCCACCA GCCTCCGCCT 
CCACCACCGA TGGGATATGA GCCACGCTCC CCATACGAGG ACGGACCTAA CAGGGATTTC 
AGCCCTCCTC AGTCCCAGTA TGACGGTGTG TCCCCAATGG GCTACGATCA CCGGCCGCGG 
CACCCCAAAC CTGCACCGGT CCGATATGAA GAGCCTCCAC CTCCACCTCC TCCGGTGTCC 
TACGATGCAC GGTCACCTTT CGAACCCGAG CCGCATGCTT TCCCAGGCAA CACACATCGC 
TCGCCAGACC CACCGAAACA GTATTATGGA GACGCCGCTA TGAGGCCCTT GTACAACCCT 
GGACCTCCGA ACAGAGTGTA CAAACATGAG CCGGTGAACT CTGAGCCTCC TGTACCGCCT 
CCCAAACCTG AATCTGTCCT GTCTCCAGGA GAACCGCCCC ATTCTGCAGC TTCCAAACCA 
TTGCCGCCTC CTCCCAGAGA TGATGACGAT GATGATGACG ATGATGATGA CCCTGCCATG 
AAGCCCCAGT CTGTGCTCAA CAGGGTCAAA ATGTTCGAAA ACAAACGCTC TGTGTCGGTA 
GACCGAGCCA AGGACGCACC TGACGTCACT GGAATTAGGC CCACAGATCT CCCCAAACCT 
GCGAGCACCC CTGGACCTGT GCTCAAAGCC AACTCTCTCA GCAACCTGGA GCAGGACAAA 
CCCTCCTACA GAGCTCCAGA GCCGCAGAAA CCTCAGACGC GTGTTGGGGA CGATGTGGTT 
CGCTCCAACC ACTACGACCC TGATGATGAT GAAGAGTACT ACAGAAAACA GCTCTCATTT 
TTCGACCGCC GCAGTTTCGA CAGTAAGGTC ATGAATCCAC CCAGCGCTGG AGTAAACCGC 
TTCCATGAGC CGCCCAAACC ACCCCAGACA CAGATCGCTT ATCCTTTCGC TAGGACGGAG 
TCTGTGGAGA AGGTGAGTCC TGTGGACAAG AGATACGAGC CTCTGCCGCC AGTCAACCCT 
TCACCTGCTC CATACAGCCA GAACACACCT GTCGCTCCGC CCACATCCCT GCCCAAACTC 
ACCAACATCG AGGTGAACTC TCTGCCAGAT CCTCACAGCT CTCCCAAAGC CAAACCGGAC 
CTGTCTGCTC TGAGAGCTCC GACCCGGGAC GAGCCCATCC AGAGCAGCTA CTTGCCCCCC 
AAGTCCACCA TCAATGGCAT GGATGCTCCT CCCAAAACAC TCGGCGTGCC GACCAGCTAC 
AACCGCTACG TCCCCAAGCC TTACACCAGC TCGGCGCGAC CCTTCGAGCG CAAGTTCGAG 
AGTCCGAAAT TCAACCACAA CCTGCTTCCC AACGACACGC AGTCCAAACC CAGTGTCAAC 
AACAACCTGA AGCCTCAGAT CTCCCCACAA CCCCAGGACA CGGACAGCGG CGTCGACACC 
TTCACTCGCA CCATGGACAA CAGGCCAAAA TATCAGCACA ACAACATCAA CGCCATTCCC 
AAAGCCGTCC CAGTCAGCCC CAGTGCACTG GATGATGATG AAGAAGATGA GGGACACACA 
GTTGTGGCCA CGGCGCGGGG CATCTTTAAC TGTAATGGCG GGGTGTTGAG CTCTATAGAG 
ACGGGCGTCA GCATCATTAT CCCACAGGGA GCCATTCCTG ATAGTGTGGA GCAGGAGATC 
TACTTCAAAG TGTGCCGGGA CAACAGCATC CTGCCTCCTC TGGATAAGGA GAAAGGTGAA 
ACTCTGCTGA GTCCGCTGGT GATGTGTGGT CCTCACGGCC TGAAGTTCCT GAAGCCTGTG 
GAGCTGCGCT TGCCACACTG TGCGTCTATG ACCCCTGATG GTTGGTCTTT TGCTCTAAAA 
TCCTCCGACT CCTCGTCGGG TGACCCCAAA TGCTGGCAAA ACAAGTGTCT ACCAGGAGAC 
CCCAATTACC TGGTGGGCGC AAACTGTGTG TCTGTGCTGA TCGACCACTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296410.1
CDS	110..6163
Translation	MLLSVFGGPVEEVLAGLLKLKMFGSGSKKWFIKKNKRQRTKVQRKPASGRRNKRVSRVLKRHKEVIVKQLDVSKVLPTLVYERVFTLGEYKEILGQDSSGKRAELFLDLLSQKEPAAFSAFCGVLEEVCPHLLTCFLLDGTDGSAGENKKQGQTKPAHSQQNALSKGQDTVCFPPMYTDPLFDTRVFPKHDKDKYGPAVYYPKFNAYIPNPDKVSVLDESEGSGHGKPSLRRIKGRIHRSKSLDSIDLLDSNSAAMDETVIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGLLQENDRVVMVNAVSMDNVDHAYAVQQLRKSGKNAKITIRRKRKVQIPSGRPGERETMSEHDEDDDSYEDEIYESRSARSGPSAYSGGGGGGGGGGASGRRSGRGDRLSGRRERDRERSGSRERSLSPRSDRHSVSSNLPPRPAKVTLVKSRKNEEYGLRLASHIFVKDISPESLAARDGNIQEGDVVLKINGTVTENLSLIDAKKLIERSKGKLKMVVQRDERATLLNIPDLDDSIPSANASDRDDISDIHSVASDHSNRSHDRKRSSRSRSPDRRSEPSDHSRHSPPQISNGSHRSRDEDRISKAGPLPLPAKMVEEMPKEQSAARETEKQLPPLPEPKPVYAQPGQPDVDLPVSPADAPVPSVTHDDSILRPSMKLVKFKKGESVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFANIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHQEVERGIIPNKNRAEQLSSVQYTLPKTPGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVVIFGPIADVAREKLSREETDLFELAKSEPRDAGTDQRSSGIIRLHTIKQIIDRDKHAVLDITPNAVDRLNYAQWYPIVVFLNPDSKQGVKNMRTRLCPESRKSARKLYERALKLRKNNHHLFTTTINLNNMNDGWYGALKESIQQQQNQLVWVSEGKADGAPDDDLDIHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETMNDEVGLPSEPAITRSSEPVREDPPVIQDAAGYPSYQHAVPQPEAVSRIDPAGFKMAAPQQQAEAALASPPPPSAAVQPTATLAGTNPEEPPGTPQADSLNSPVPVPDPQPEPELAQPPTHDPHQSLPPGPDPKMYKKDLYSMDEPVRVTHGVKPPPPQQQQQPLGPPTSLSYSHQPVYQDQQPYRQYDHPPYGYDGGGYAQPKPHNYDPHLHYDNRVPHYNEQWPTYDQQQQQQTSPQPPPASGFPQGHQPPPPPPMGYEPRSPYEDGPNRDFSPPQSQYDGVSPMGYDHRPRHPKPAPVRYEEPPPPPPPVSYDARSPFEPEPHAFPGNTHRSPDPPKQYYGDAAMRPLYNPGPPNRVYKHEPVNSEPPVPPPKPESVLSPGEPPHSAASKPLPPPPRDDDDDDDDDDDPAMKPQSVLNRVKMFENKRSVSVDRAKDAPDVTGIRPTDLPKPASTPGPVLKANSLSNLEQDKPSYRAPEPQKPQTRVGDDVVRSNHYDPDDDEEYYRKQLSFFDRRSFDSKVMNPPSAGVNRFHEPPKPPQTQIAYPFARTESVEKVSPVDKRYEPLPPVNPSPAPYSQNTPVAPPTSLPKLTNIEVNSLPDPHSSPKAKPDLSALRAPTRDEPIQSSYLPPKSTINGMDAPPKTLGVPTSYNRYVPKPYTSSARPFERKFESPKFNHNLLPNDTQSKPSVNNNLKPQISPQPQDTDSGVDTFTRTMDNRPKYQHNNINAIPKAVPVSPSALDDDEEDEGHTVVATARGIFNCNGGVLSSIETGVSIIIPQGAIPDSVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKCWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_009298135.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tight junction protein 1b (tjp1b), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298135.3

ORF Insert Sequence:

ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
TCCCGCAAGA ATGAAGAATA CGGGCTTCGC CTGGCCAGCC ACATCTTCGT GAAGGACATT 
TCCCCCGAGA GCCTGGCAGC CCGAGACGGA AACATCCAAG AGGGAGACGT GGTGCTTAAG 
ATTAATGGCA CGGTAACAGA GAATTTGTCC CTGATCGATG CTAAGAAGCT GATCGAGAGG 
TCAAAGGGAA AGCTGAAGAT GGTGGTGCAG AGAGACGAGC GGGCGACGCT GCTGAACATC 
CCCGATCTGG ACGACAGCAT ACCTTCTGCT AATGCCTCGG ACAGAGATGA TATTTCAGAC 
ATCCACTCCG TGGCCTCTGA TCATTCCAAC CGATCCCACG ACCGGAAGAG GAGCAGCCGC 
TCTCGTTCTC CAGACCGCCG CTCCGAACCC TCAGACCACT CCAGACACTC TCCACCACAG 
ATCAGCAACG GCAGTCACAG GAGCCGTGAT GAAGACAGGA TCTCCAAGGC CGGGCCGCTT 
CCTCTACCTG CTAAGATGGT GGAGGAGATG CCGAAAGAGC AGAGCGCAGC CAGAGAAACA 
GAGAAACAGC TCCCCCCTCT GCCAGAGCCC AAACCTGTGT ACGCTCAGCC GGGCCAGCCG 
GACGTGGACC TGCCTGTTAG TCCGGCAGAT GCTCCTGTGC CCAGCGTCAC ACATGATGAC 
AGCATCTTAC GGCCGAGCAT GAAGCTGGTG AAGTTCAAGA AGGGCGAGAG CGTGGGCTTG 
AGGCTCGCTG GGGGGAACGA CGTTGGGATA TTTGTGGCGG GAGTTCTGGA GGACAGTCCT 
GCTGCTAAAG AGGGCCTGGA AGAGGGAGAC CAGATTCTCA GGGTAAATAA TGTGGATTTT 
GCAAATATCA TCCGAGAGGA GGCAGTGCTG TTCCTGCTGG ATCTGCCCAA AGGAGAGGAG 
GTCACCATCC TGGCCCAGAA GAAGAAAGAC GTGTACCGCC GCATTGTAGA GTCTGACGTG 
GGAGACTCGT TCTACATCAG GACTCATTTT GAATATGAGA AGGAGTCGCC CTATGGCCTG 
AGCTTCAATA AGGGGGAGGT CTTCAGAGTC GTCGACACCC TTTACAATGG CAAACTGGGC 
TCCTGGCTCG CCATCCGCAT CGGCAAAAAC CACCAGGAGG TGGAGCGGGG CATCATCCCC 
AACAAGAACA GGGCTGAGCA GTTATCAAGT GTCCAGTACA CTCTCCCAAA GACTCCAGGA 
GGGGATCGCG CCGACTTCTG GAGATTCAGA GGCCTCCGCA GCTCCAAACG AAACCTGAGG 
AAGAGCAGAG AAGACCTGTC TGCTCAGCCG GTCCAAACCA AATTCCCTGC TTATGAAAGA 
GTGGTGCTTA GAGAAGCTGG TTTCTTGAGG CCAGTGGTCA TTTTCGGACC GATCGCGGAT 
GTGGCTAGAG AGAAGCTGTC CAGAGAGGAG ACTGACCTCT TCGAACTTGC AAAGAGTGAA 
CCCAGAGATG CGGGCACAGA CCAGCGCAGC TCCGGAATCA TCCGTCTCCA TACCATTAAA 
CAGATCATTG ACCGAGACAA GCACGCGGTG CTGGACATCA CCCCTAATGC GGTGGACCGT 
CTGAACTACG CTCAGTGGTA CCCCATCGTG GTGTTTCTGA ATCCAGACAG CAAACAGGGC 
GTCAAGAACA TGAGGACCAG GCTCTGTCCC GAGTCCAGGA AGAGCGCCCG AAAGCTCTAC 
GAGCGTGCTC TCAAACTGAG GAAGAACAAC CATCACCTTT TCACCACCAC TATTAACTTG 
AACAACATGA ATGACGGGTG GTACGGTGCT TTGAAAGAGA GCATCCAGCA GCAGCAGAAC 
CAGTTGGTCT GGGTCTCAGA GGGCAAGGCC GACGGCGCTC CTGACGATGA CCTTGACATC 
CATGACGACC GCCTCTCCTA CCTCTCGGCT CCGGGCAGCG AGTACAGCAT GTACAGCACC 
GACAGCCGCC ACACGTCCGA CTATGAGGAC ACCGACACGG AGGGTGGTGC CTACACCGAC 
CAGGAGCTGG ACGAGACCAT GAACGATGAG GTGGGCCTGC CTAGCGAGCC TGCCATCACA 
CGCTCATCTG AGCCTGTGCG AGAGGACCCG CCAGTCATTC AGGACGCAGC TGGATACCCC 
AGCTACCAGC ATGCGGTACC GCAACCTGAA GCGGTGAGCC GCATTGACCC GGCCGGGTTT 
AAGATGGCAG CGCCGCAGCA GCAAGCTGAG GCTGCTCTGG CCTCGCCGCC CCCTCCCTCT 
GCAGCGGTCC AGCCCACCGC AACACTAGCG GGTACGAACC CCGAGGAGCC GCCTGGCACC 
CCTCAGGCCG ACTCCCTTAA CAGCCCCGTC CCTGTTCCCG ACCCCCAGCC CGAACCTGAG 
CTCGCTCAGC CCCCAACACA CGACCCCCAC CAGTCGCTTC CTCCTGGCCC AGATCCAAAG 
ATGTATAAAA AGGATCTGTA CAGCATGGAC GAGCCGGTGC GAGTGACCCA CGGCGTGAAG 
CCTCCCCCTC CCCAGCAGCA GCAGCAGCCT TTAGGCCCCC CAACCTCGCT CTCCTACAGC 
CACCAGCCTG TCTACCAGGA CCAACAGCCA TACCGCCAGT ACGACCACCC GCCTTATGGC 
TATGACGGTG GCGGCTACGC ACAACCAAAG CCTCACAACT ACGACCCGCA CCTGCACTAC 
GACAACCGTG TGCCTCACTA TAACGAGCAG TGGCCCACGT ACGACCAGCA GCAGCAGCAG 
CAGACTTCCC CCCAGCCGCC ACCTGCGTCC GGCTTCCCTC AGGGCCACCA GCCTCCGCCT 
CCACCACCGA TGGGATATGA GCCACGCTCC CCATACGAGG ACGGACCTAA CAGGGATTTC 
AGCCCTCCTC AGTCCCAGTA TGACGGTGTG TCCCCAATGG GCTACGATCA CCGGCCGCGG 
CACCCCAAAC CTGCACCGGT CCGATATGAA GAGCCTCCAC CTCCACCTCC TCCGGTGTCC 
TACGATGCAC GGTCACCTTT CGAACCCGAG CCGCATGCTT TCCCAGGCAA CACACATCGC 
TCGCCAGACC CACCGAAACA GTATTATGGA GACGCCGCTA TGAGGCCCTT GTACAACCCT 
GGACCTCCGA ACAGAGTGTA CAAACATGAG CCGGTGAACT CTGAGCCTCC TGTACCGCCT 
CCCAAACCTG AATCTGTCCT GTCTCCAGGA GAACCGCCCC ATTCTGCAGC TTCCAAACCA 
TTGCCGCCTC CTCCCAGAGA TGATGACGAT GATGATGACG ATGATGATGA CCCTGCCATG 
AAGCCCCAGT CTGTGCTCAA CAGGGTCAAA ATGTTCGAAA ACAAACGCTC TGTGTCGGTA 
GACCGAGCCA AGGACGCACC TGACGTCACT GGAATTAGGC CCACAGATCT CCCCAAACCT 
GCGAGCACCC CTGGACCTGT GCTCAAAGCC AACTCTCTCA GCAACCTGGA GCAGGACAAA 
CCCTCCTACA GAGCTCCAGA GCCGCAGAAA CCTCAGACGC GTGTTGGGGA CGATGTGGTT 
CGCTCCAACC ACTACGACCC TGATGATGAT GAAGAGTACT ACAGAAAACA GCTCTCATTT 
TTCGACCGCC GCAGTTTCGA CAGTAAGGTC ATGAATCCAC CCAGCGCTGG AGTAAACCGC 
TTCCATGAGC CGCCCAAACC ACCCCAGACA CAGATCGCTT ATCCTTTCGC TAGGACGGAG 
TCTGTGGAGA AGGTGAGTCC TGTGGACAAG AGATACGAGC CTCTGCCGCC AGTCAACCCT 
TCACCTGCTC CATACAGCCA GAACACACCT GTCGCTCCGC CCACATCCCT GCCCAAACTC 
ACCAACATCG AGGTGAACTC TCTGCCAGAT CCTCACAGCT CTCCCAAAGC CAAACCGGAC 
CTGTCTGCTC TGAGAGCTCC GACCCGGGAC GAGCCCATCC AGAGCAGCTA CTTGCCCCCC 
AAGTCCACCA TCAATGGCAT GGATGCTCCT CCCAAAACAC TCGGCGTGCC GACCAGCTAC 
AACCGCTACG TCCCCAAGCC TTACACCAGC TCGGCGCGAC CCTTCGAGCG CAAGTTCGAG 
AGTCCGAAAT TCAACCACAA CCTGCTTCCC AACGACACGC AGTCCAAACC CAGTGTCAAC 
AACAACCTGA AGCCTCAGAT CTCCCCACAA CCCCAGGACA CGGACAGCGG CGTCGACACC 
TTCACTCGCA CCATGGACAA CAGGCCAAAA TATCAGCACA ACAACATCAA CGCCATTCCC 
AAAGCCGTCC CAGTCAGCCC CAGTGCACTG GATGATGATG AAGAAGATGA GGGACACACA 
GTTGTGGCCA CGGCGCGGGG CATCTTTAAC TGTAATGGCG GGGTGTTGAG CTCTATAGAG 
ACGGGCGTCA GCATCATTAT CCCACAGGGA GCCATTCCTG ATAGTGTGGA GCAGGAGATC 
TACTTCAAAG TGTGCCGGGA CAACAGCATC CTGCCTCCTC TGGATAAGGA GAAAGGTGAA 
ACTCTGCTGA GTCCGCTGGT GATGTGTGGT CCTCACGGCC TGAAGTTCCT GAAGCCTGTG 
GAGCTGCGCT TGCCACACTG TGCGTCTATG ACCCCTGATG GTTGGTCTTT TGCTCTAAAA 
TCCTCCGACT CCTCGTCGGG TGACCCCAAA TGCTGGCAAA ACAAGTGTCT ACCAGGAGAC 
CCCAATTACC TGGTGGGCGC AAACTGTGTG TCTGTGCTGA TCGACCACTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp1b ( XM_009298135.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296410.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X6, mRNA.

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CloneID	ODa44625
Clone ID Related Accession (Same CDS sequence)	XM_009298135.3 , XM_009298135.2
Accession Version	XM_009298135.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tight junction protein ZO-1 isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298135.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
TCCCGCAAGA ATGAAGAATA CGGGCTTCGC CTGGCCAGCC ACATCTTCGT GAAGGACATT 
TCCCCCGAGA GCCTGGCAGC CCGAGACGGA AACATCCAAG AGGGAGACGT GGTGCTTAAG 
ATTAATGGCA CGGTAACAGA GAATTTGTCC CTGATCGATG CTAAGAAGCT GATCGAGAGG 
TCAAAGGGAA AGCTGAAGAT GGTGGTGCAG AGAGACGAGC GGGCGACGCT GCTGAACATC 
CCCGATCTGG ACGACAGCAT ACCTTCTGCT AATGCCTCGG ACAGAGATGA TATTTCAGAC 
ATCCACTCCG TGGCCTCTGA TCATTCCAAC CGATCCCACG ACCGGAAGAG GAGCAGCCGC 
TCTCGTTCTC CAGACCGCCG CTCCGAACCC TCAGACCACT CCAGACACTC TCCACCACAG 
ATCAGCAACG GCAGTCACAG GAGCCGTGAT GAAGACAGGA TCTCCAAGGC CGGGCCGCTT 
CCTCTACCTG CTAAGATGGT GGAGGAGATG CCGAAAGAGC AGAGCGCAGC CAGAGAAACA 
GAGAAACAGC TCCCCCCTCT GCCAGAGCCC AAACCTGTGT ACGCTCAGCC GGGCCAGCCG 
GACGTGGACC TGCCTGTTAG TCCGGCAGAT GCTCCTGTGC CCAGCGTCAC ACATGATGAC 
AGCATCTTAC GGCCGAGCAT GAAGCTGGTG AAGTTCAAGA AGGGCGAGAG CGTGGGCTTG 
AGGCTCGCTG GGGGGAACGA CGTTGGGATA TTTGTGGCGG GAGTTCTGGA GGACAGTCCT 
GCTGCTAAAG AGGGCCTGGA AGAGGGAGAC CAGATTCTCA GGGTAAATAA TGTGGATTTT 
GCAAATATCA TCCGAGAGGA GGCAGTGCTG TTCCTGCTGG ATCTGCCCAA AGGAGAGGAG 
GTCACCATCC TGGCCCAGAA GAAGAAAGAC GTGTACCGCC GCATTGTAGA GTCTGACGTG 
GGAGACTCGT TCTACATCAG GACTCATTTT GAATATGAGA AGGAGTCGCC CTATGGCCTG 
AGCTTCAATA AGGGGGAGGT CTTCAGAGTC GTCGACACCC TTTACAATGG CAAACTGGGC 
TCCTGGCTCG CCATCCGCAT CGGCAAAAAC CACCAGGAGG TGGAGCGGGG CATCATCCCC 
AACAAGAACA GGGCTGAGCA GTTATCAAGT GTCCAGTACA CTCTCCCAAA GACTCCAGGA 
GGGGATCGCG CCGACTTCTG GAGATTCAGA GGCCTCCGCA GCTCCAAACG AAACCTGAGG 
AAGAGCAGAG AAGACCTGTC TGCTCAGCCG GTCCAAACCA AATTCCCTGC TTATGAAAGA 
GTGGTGCTTA GAGAAGCTGG TTTCTTGAGG CCAGTGGTCA TTTTCGGACC GATCGCGGAT 
GTGGCTAGAG AGAAGCTGTC CAGAGAGGAG ACTGACCTCT TCGAACTTGC AAAGAGTGAA 
CCCAGAGATG CGGGCACAGA CCAGCGCAGC TCCGGAATCA TCCGTCTCCA TACCATTAAA 
CAGATCATTG ACCGAGACAA GCACGCGGTG CTGGACATCA CCCCTAATGC GGTGGACCGT 
CTGAACTACG CTCAGTGGTA CCCCATCGTG GTGTTTCTGA ATCCAGACAG CAAACAGGGC 
GTCAAGAACA TGAGGACCAG GCTCTGTCCC GAGTCCAGGA AGAGCGCCCG AAAGCTCTAC 
GAGCGTGCTC TCAAACTGAG GAAGAACAAC CATCACCTTT TCACCACCAC TATTAACTTG 
AACAACATGA ATGACGGGTG GTACGGTGCT TTGAAAGAGA GCATCCAGCA GCAGCAGAAC 
CAGTTGGTCT GGGTCTCAGA GGGCAAGGCC GACGGCGCTC CTGACGATGA CCTTGACATC 
CATGACGACC GCCTCTCCTA CCTCTCGGCT CCGGGCAGCG AGTACAGCAT GTACAGCACC 
GACAGCCGCC ACACGTCCGA CTATGAGGAC ACCGACACGG AGGGTGGTGC CTACACCGAC 
CAGGAGCTGG ACGAGACCAT GAACGATGAG GTGGGCCTGC CTAGCGAGCC TGCCATCACA 
CGCTCATCTG AGCCTGTGCG AGAGGACCCG CCAGTCATTC AGGACGCAGC TGGATACCCC 
AGCTACCAGC ATGCGGTACC GCAACCTGAA GCGGTGAGCC GCATTGACCC GGCCGGGTTT 
AAGATGGCAG CGCCGCAGCA GCAAGCTGAG GCTGCTCTGG CCTCGCCGCC CCCTCCCTCT 
GCAGCGGTCC AGCCCACCGC AACACTAGCG GGTACGAACC CCGAGGAGCC GCCTGGCACC 
CCTCAGGCCG ACTCCCTTAA CAGCCCCGTC CCTGTTCCCG ACCCCCAGCC CGAACCTGAG 
CTCGCTCAGC CCCCAACACA CGACCCCCAC CAGTCGCTTC CTCCTGGCCC AGATCCAAAG 
ATGTATAAAA AGGATCTGTA CAGCATGGAC GAGCCGGTGC GAGTGACCCA CGGCGTGAAG 
CCTCCCCCTC CCCAGCAGCA GCAGCAGCCT TTAGGCCCCC CAACCTCGCT CTCCTACAGC 
CACCAGCCTG TCTACCAGGA CCAACAGCCA TACCGCCAGT ACGACCACCC GCCTTATGGC 
TATGACGGTG GCGGCTACGC ACAACCAAAG CCTCACAACT ACGACCCGCA CCTGCACTAC 
GACAACCGTG TGCCTCACTA TAACGAGCAG TGGCCCACGT ACGACCAGCA GCAGCAGCAG 
CAGACTTCCC CCCAGCCGCC ACCTGCGTCC GGCTTCCCTC AGGGCCACCA GCCTCCGCCT 
CCACCACCGA TGGGATATGA GCCACGCTCC CCATACGAGG ACGGACCTAA CAGGGATTTC 
AGCCCTCCTC AGTCCCAGTA TGACGGTGTG TCCCCAATGG GCTACGATCA CCGGCCGCGG 
CACCCCAAAC CTGCACCGGT CCGATATGAA GAGCCTCCAC CTCCACCTCC TCCGGTGTCC 
TACGATGCAC GGTCACCTTT CGAACCCGAG CCGCATGCTT TCCCAGGCAA CACACATCGC 
TCGCCAGACC CACCGAAACA GTATTATGGA GACGCCGCTA TGAGGCCCTT GTACAACCCT 
GGACCTCCGA ACAGAGTGTA CAAACATGAG CCGGTGAACT CTGAGCCTCC TGTACCGCCT 
CCCAAACCTG AATCTGTCCT GTCTCCAGGA GAACCGCCCC ATTCTGCAGC TTCCAAACCA 
TTGCCGCCTC CTCCCAGAGA TGATGACGAT GATGATGACG ATGATGATGA CCCTGCCATG 
AAGCCCCAGT CTGTGCTCAA CAGGGTCAAA ATGTTCGAAA ACAAACGCTC TGTGTCGGTA 
GACCGAGCCA AGGACGCACC TGACGTCACT GGAATTAGGC CCACAGATCT CCCCAAACCT 
GCGAGCACCC CTGGACCTGT GCTCAAAGCC AACTCTCTCA GCAACCTGGA GCAGGACAAA 
CCCTCCTACA GAGCTCCAGA GCCGCAGAAA CCTCAGACGC GTGTTGGGGA CGATGTGGTT 
CGCTCCAACC ACTACGACCC TGATGATGAT GAAGAGTACT ACAGAAAACA GCTCTCATTT 
TTCGACCGCC GCAGTTTCGA CAGTAAGGTC ATGAATCCAC CCAGCGCTGG AGTAAACCGC 
TTCCATGAGC CGCCCAAACC ACCCCAGACA CAGATCGCTT ATCCTTTCGC TAGGACGGAG 
TCTGTGGAGA AGGTGAGTCC TGTGGACAAG AGATACGAGC CTCTGCCGCC AGTCAACCCT 
TCACCTGCTC CATACAGCCA GAACACACCT GTCGCTCCGC CCACATCCCT GCCCAAACTC 
ACCAACATCG AGGTGAACTC TCTGCCAGAT CCTCACAGCT CTCCCAAAGC CAAACCGGAC 
CTGTCTGCTC TGAGAGCTCC GACCCGGGAC GAGCCCATCC AGAGCAGCTA CTTGCCCCCC 
AAGTCCACCA TCAATGGCAT GGATGCTCCT CCCAAAACAC TCGGCGTGCC GACCAGCTAC 
AACCGCTACG TCCCCAAGCC TTACACCAGC TCGGCGCGAC CCTTCGAGCG CAAGTTCGAG 
AGTCCGAAAT TCAACCACAA CCTGCTTCCC AACGACACGC AGTCCAAACC CAGTGTCAAC 
AACAACCTGA AGCCTCAGAT CTCCCCACAA CCCCAGGACA CGGACAGCGG CGTCGACACC 
TTCACTCGCA CCATGGACAA CAGGCCAAAA TATCAGCACA ACAACATCAA CGCCATTCCC 
AAAGCCGTCC CAGTCAGCCC CAGTGCACTG GATGATGATG AAGAAGATGA GGGACACACA 
GTTGTGGCCA CGGCGCGGGG CATCTTTAAC TGTAATGGCG GGGTGTTGAG CTCTATAGAG 
ACGGGCGTCA GCATCATTAT CCCACAGGGA GCCATTCCTG ATAGTGTGGA GCAGGAGATC 
TACTTCAAAG TGTGCCGGGA CAACAGCATC CTGCCTCCTC TGGATAAGGA GAAAGGTGAA 
ACTCTGCTGA GTCCGCTGGT GATGTGTGGT CCTCACGGCC TGAAGTTCCT GAAGCCTGTG 
GAGCTGCGCT TGCCACACTG TGCGTCTATG ACCCCTGATG GTTGGTCTTT TGCTCTAAAA 
TCCTCCGACT CCTCGTCGGG TGACCCCAAA TGCTGGCAAA ACAAGTGTCT ACCAGGAGAC 
CCCAATTACC TGGTGGGCGC AAACTGTGTG TCTGTGCTGA TCGACCACTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296410.1
CDS	115..6168
Translation	MLLSVFGGPVEEVLAGLLKLKMFGSGSKKWFIKKNKRQRTKVQRKPASGRRNKRVSRVLKRHKEVIVKQLDVSKVLPTLVYERVFTLGEYKEILGQDSSGKRAELFLDLLSQKEPAAFSAFCGVLEEVCPHLLTCFLLDGTDGSAGENKKQGQTKPAHSQQNALSKGQDTVCFPPMYTDPLFDTRVFPKHDKDKYGPAVYYPKFNAYIPNPDKVSVLDESEGSGHGKPSLRRIKGRIHRSKSLDSIDLLDSNSAAMDETVIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGLLQENDRVVMVNAVSMDNVDHAYAVQQLRKSGKNAKITIRRKRKVQIPSGRPGERETMSEHDEDDDSYEDEIYESRSARSGPSAYSGGGGGGGGGGASGRRSGRGDRLSGRRERDRERSGSRERSLSPRSDRHSVSSNLPPRPAKVTLVKSRKNEEYGLRLASHIFVKDISPESLAARDGNIQEGDVVLKINGTVTENLSLIDAKKLIERSKGKLKMVVQRDERATLLNIPDLDDSIPSANASDRDDISDIHSVASDHSNRSHDRKRSSRSRSPDRRSEPSDHSRHSPPQISNGSHRSRDEDRISKAGPLPLPAKMVEEMPKEQSAARETEKQLPPLPEPKPVYAQPGQPDVDLPVSPADAPVPSVTHDDSILRPSMKLVKFKKGESVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFANIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHQEVERGIIPNKNRAEQLSSVQYTLPKTPGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVVIFGPIADVAREKLSREETDLFELAKSEPRDAGTDQRSSGIIRLHTIKQIIDRDKHAVLDITPNAVDRLNYAQWYPIVVFLNPDSKQGVKNMRTRLCPESRKSARKLYERALKLRKNNHHLFTTTINLNNMNDGWYGALKESIQQQQNQLVWVSEGKADGAPDDDLDIHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETMNDEVGLPSEPAITRSSEPVREDPPVIQDAAGYPSYQHAVPQPEAVSRIDPAGFKMAAPQQQAEAALASPPPPSAAVQPTATLAGTNPEEPPGTPQADSLNSPVPVPDPQPEPELAQPPTHDPHQSLPPGPDPKMYKKDLYSMDEPVRVTHGVKPPPPQQQQQPLGPPTSLSYSHQPVYQDQQPYRQYDHPPYGYDGGGYAQPKPHNYDPHLHYDNRVPHYNEQWPTYDQQQQQQTSPQPPPASGFPQGHQPPPPPPMGYEPRSPYEDGPNRDFSPPQSQYDGVSPMGYDHRPRHPKPAPVRYEEPPPPPPPVSYDARSPFEPEPHAFPGNTHRSPDPPKQYYGDAAMRPLYNPGPPNRVYKHEPVNSEPPVPPPKPESVLSPGEPPHSAASKPLPPPPRDDDDDDDDDDDPAMKPQSVLNRVKMFENKRSVSVDRAKDAPDVTGIRPTDLPKPASTPGPVLKANSLSNLEQDKPSYRAPEPQKPQTRVGDDVVRSNHYDPDDDEEYYRKQLSFFDRRSFDSKVMNPPSAGVNRFHEPPKPPQTQIAYPFARTESVEKVSPVDKRYEPLPPVNPSPAPYSQNTPVAPPTSLPKLTNIEVNSLPDPHSSPKAKPDLSALRAPTRDEPIQSSYLPPKSTINGMDAPPKTLGVPTSYNRYVPKPYTSSARPFERKFESPKFNHNLLPNDTQSKPSVNNNLKPQISPQPQDTDSGVDTFTRTMDNRPKYQHNNINAIPKAVPVSPSALDDDEEDEGHTVVATARGIFNCNGGVLSSIETGVSIIIPQGAIPDSVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKCWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_009298135.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tight junction protein 1b (tjp1b), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298135.2

ORF Insert Sequence:

ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
TCCCGCAAGA ATGAAGAATA CGGGCTTCGC CTGGCCAGCC ACATCTTCGT GAAGGACATT 
TCCCCCGAGA GCCTGGCAGC CCGAGACGGA AACATCCAAG AGGGAGACGT GGTGCTTAAG 
ATTAATGGCA CGGTAACAGA GAATTTGTCC CTGATCGATG CTAAGAAGCT GATCGAGAGG 
TCAAAGGGAA AGCTGAAGAT GGTGGTGCAG AGAGACGAGC GGGCGACGCT GCTGAACATC 
CCCGATCTGG ACGACAGCAT ACCTTCTGCT AATGCCTCGG ACAGAGATGA TATTTCAGAC 
ATCCACTCCG TGGCCTCTGA TCATTCCAAC CGATCCCACG ACCGGAAGAG GAGCAGCCGC 
TCTCGTTCTC CAGACCGCCG CTCCGAACCC TCAGACCACT CCAGACACTC TCCACCACAG 
ATCAGCAACG GCAGTCACAG GAGCCGTGAT GAAGACAGGA TCTCCAAGGC CGGGCCGCTT 
CCTCTACCTG CTAAGATGGT GGAGGAGATG CCGAAAGAGC AGAGCGCAGC CAGAGAAACA 
GAGAAACAGC TCCCCCCTCT GCCAGAGCCC AAACCTGTGT ACGCTCAGCC GGGCCAGCCG 
GACGTGGACC TGCCTGTTAG TCCGGCAGAT GCTCCTGTGC CCAGCGTCAC ACATGATGAC 
AGCATCTTAC GGCCGAGCAT GAAGCTGGTG AAGTTCAAGA AGGGCGAGAG CGTGGGCTTG 
AGGCTCGCTG GGGGGAACGA CGTTGGGATA TTTGTGGCGG GAGTTCTGGA GGACAGTCCT 
GCTGCTAAAG AGGGCCTGGA AGAGGGAGAC CAGATTCTCA GGGTAAATAA TGTGGATTTT 
GCAAATATCA TCCGAGAGGA GGCAGTGCTG TTCCTGCTGG ATCTGCCCAA AGGAGAGGAG 
GTCACCATCC TGGCCCAGAA GAAGAAAGAC GTGTACCGCC GCATTGTAGA GTCTGACGTG 
GGAGACTCGT TCTACATCAG GACTCATTTT GAATATGAGA AGGAGTCGCC CTATGGCCTG 
AGCTTCAATA AGGGGGAGGT CTTCAGAGTC GTCGACACCC TTTACAATGG CAAACTGGGC 
TCCTGGCTCG CCATCCGCAT CGGCAAAAAC CACCAGGAGG TGGAGCGGGG CATCATCCCC 
AACAAGAACA GGGCTGAGCA GTTATCAAGT GTCCAGTACA CTCTCCCAAA GACTCCAGGA 
GGGGATCGCG CCGACTTCTG GAGATTCAGA GGCCTCCGCA GCTCCAAACG AAACCTGAGG 
AAGAGCAGAG AAGACCTGTC TGCTCAGCCG GTCCAAACCA AATTCCCTGC TTATGAAAGA 
GTGGTGCTTA GAGAAGCTGG TTTCTTGAGG CCAGTGGTCA TTTTCGGACC GATCGCGGAT 
GTGGCTAGAG AGAAGCTGTC CAGAGAGGAG ACTGACCTCT TCGAACTTGC AAAGAGTGAA 
CCCAGAGATG CGGGCACAGA CCAGCGCAGC TCCGGAATCA TCCGTCTCCA TACCATTAAA 
CAGATCATTG ACCGAGACAA GCACGCGGTG CTGGACATCA CCCCTAATGC GGTGGACCGT 
CTGAACTACG CTCAGTGGTA CCCCATCGTG GTGTTTCTGA ATCCAGACAG CAAACAGGGC 
GTCAAGAACA TGAGGACCAG GCTCTGTCCC GAGTCCAGGA AGAGCGCCCG AAAGCTCTAC 
GAGCGTGCTC TCAAACTGAG GAAGAACAAC CATCACCTTT TCACCACCAC TATTAACTTG 
AACAACATGA ATGACGGGTG GTACGGTGCT TTGAAAGAGA GCATCCAGCA GCAGCAGAAC 
CAGTTGGTCT GGGTCTCAGA GGGCAAGGCC GACGGCGCTC CTGACGATGA CCTTGACATC 
CATGACGACC GCCTCTCCTA CCTCTCGGCT CCGGGCAGCG AGTACAGCAT GTACAGCACC 
GACAGCCGCC ACACGTCCGA CTATGAGGAC ACCGACACGG AGGGTGGTGC CTACACCGAC 
CAGGAGCTGG ACGAGACCAT GAACGATGAG GTGGGCCTGC CTAGCGAGCC TGCCATCACA 
CGCTCATCTG AGCCTGTGCG AGAGGACCCG CCAGTCATTC AGGACGCAGC TGGATACCCC 
AGCTACCAGC ATGCGGTACC GCAACCTGAA GCGGTGAGCC GCATTGACCC GGCCGGGTTT 
AAGATGGCAG CGCCGCAGCA GCAAGCTGAG GCTGCTCTGG CCTCGCCGCC CCCTCCCTCT 
GCAGCGGTCC AGCCCACCGC AACACTAGCG GGTACGAACC CCGAGGAGCC GCCTGGCACC 
CCTCAGGCCG ACTCCCTTAA CAGCCCCGTC CCTGTTCCCG ACCCCCAGCC CGAACCTGAG 
CTCGCTCAGC CCCCAACACA CGACCCCCAC CAGTCGCTTC CTCCTGGCCC AGATCCAAAG 
ATGTATAAAA AGGATCTGTA CAGCATGGAC GAGCCGGTGC GAGTGACCCA CGGCGTGAAG 
CCTCCCCCTC CCCAGCAGCA GCAGCAGCCT TTAGGCCCCC CAACCTCGCT CTCCTACAGC 
CACCAGCCTG TCTACCAGGA CCAACAGCCA TACCGCCAGT ACGACCACCC GCCTTATGGC 
TATGACGGTG GCGGCTACGC ACAACCAAAG CCTCACAACT ACGACCCGCA CCTGCACTAC 
GACAACCGTG TGCCTCACTA TAACGAGCAG TGGCCCACGT ACGACCAGCA GCAGCAGCAG 
CAGACTTCCC CCCAGCCGCC ACCTGCGTCC GGCTTCCCTC AGGGCCACCA GCCTCCGCCT 
CCACCACCGA TGGGATATGA GCCACGCTCC CCATACGAGG ACGGACCTAA CAGGGATTTC 
AGCCCTCCTC AGTCCCAGTA TGACGGTGTG TCCCCAATGG GCTACGATCA CCGGCCGCGG 
CACCCCAAAC CTGCACCGGT CCGATATGAA GAGCCTCCAC CTCCACCTCC TCCGGTGTCC 
TACGATGCAC GGTCACCTTT CGAACCCGAG CCGCATGCTT TCCCAGGCAA CACACATCGC 
TCGCCAGACC CACCGAAACA GTATTATGGA GACGCCGCTA TGAGGCCCTT GTACAACCCT 
GGACCTCCGA ACAGAGTGTA CAAACATGAG CCGGTGAACT CTGAGCCTCC TGTACCGCCT 
CCCAAACCTG AATCTGTCCT GTCTCCAGGA GAACCGCCCC ATTCTGCAGC TTCCAAACCA 
TTGCCGCCTC CTCCCAGAGA TGATGACGAT GATGATGACG ATGATGATGA CCCTGCCATG 
AAGCCCCAGT CTGTGCTCAA CAGGGTCAAA ATGTTCGAAA ACAAACGCTC TGTGTCGGTA 
GACCGAGCCA AGGACGCACC TGACGTCACT GGAATTAGGC CCACAGATCT CCCCAAACCT 
GCGAGCACCC CTGGACCTGT GCTCAAAGCC AACTCTCTCA GCAACCTGGA GCAGGACAAA 
CCCTCCTACA GAGCTCCAGA GCCGCAGAAA CCTCAGACGC GTGTTGGGGA CGATGTGGTT 
CGCTCCAACC ACTACGACCC TGATGATGAT GAAGAGTACT ACAGAAAACA GCTCTCATTT 
TTCGACCGCC GCAGTTTCGA CAGTAAGGTC ATGAATCCAC CCAGCGCTGG AGTAAACCGC 
TTCCATGAGC CGCCCAAACC ACCCCAGACA CAGATCGCTT ATCCTTTCGC TAGGACGGAG 
TCTGTGGAGA AGGTGAGTCC TGTGGACAAG AGATACGAGC CTCTGCCGCC AGTCAACCCT 
TCACCTGCTC CATACAGCCA GAACACACCT GTCGCTCCGC CCACATCCCT GCCCAAACTC 
ACCAACATCG AGGTGAACTC TCTGCCAGAT CCTCACAGCT CTCCCAAAGC CAAACCGGAC 
CTGTCTGCTC TGAGAGCTCC GACCCGGGAC GAGCCCATCC AGAGCAGCTA CTTGCCCCCC 
AAGTCCACCA TCAATGGCAT GGATGCTCCT CCCAAAACAC TCGGCGTGCC GACCAGCTAC 
AACCGCTACG TCCCCAAGCC TTACACCAGC TCGGCGCGAC CCTTCGAGCG CAAGTTCGAG 
AGTCCGAAAT TCAACCACAA CCTGCTTCCC AACGACACGC AGTCCAAACC CAGTGTCAAC 
AACAACCTGA AGCCTCAGAT CTCCCCACAA CCCCAGGACA CGGACAGCGG CGTCGACACC 
TTCACTCGCA CCATGGACAA CAGGCCAAAA TATCAGCACA ACAACATCAA CGCCATTCCC 
AAAGCCGTCC CAGTCAGCCC CAGTGCACTG GATGATGATG AAGAAGATGA GGGACACACA 
GTTGTGGCCA CGGCGCGGGG CATCTTTAAC TGTAATGGCG GGGTGTTGAG CTCTATAGAG 
ACGGGCGTCA GCATCATTAT CCCACAGGGA GCCATTCCTG ATAGTGTGGA GCAGGAGATC 
TACTTCAAAG TGTGCCGGGA CAACAGCATC CTGCCTCCTC TGGATAAGGA GAAAGGTGAA 
ACTCTGCTGA GTCCGCTGGT GATGTGTGGT CCTCACGGCC TGAAGTTCCT GAAGCCTGTG 
GAGCTGCGCT TGCCACACTG TGCGTCTATG ACCCCTGATG GTTGGTCTTT TGCTCTAAAA 
TCCTCCGACT CCTCGTCGGG TGACCCCAAA TGCTGGCAAA ACAAGTGTCT ACCAGGAGAC 
CCCAATTACC TGGTGGGCGC AAACTGTGTG TCTGTGCTGA TCGACCACTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp1b ( XM_021470733.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326408.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X15, mRNA.

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CloneID	ODa44626
Clone ID Related Accession (Same CDS sequence)	XM_021470733.1
Accession Version	XM_021470733.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5913bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tight junction protein ZO-1 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
TCCCGCAAGA ATGAAGCATG TATGGCGTCA GGAAGTTCTC AGAAGGTGAC ATCAGCAGAA 
TACGGGCTTC GCCTGGCCAG CCACATCTTC GTGAAGGACA TTTCCCCCGA GAGCCTGGCA 
GCCCGAGACG GAAACATCCA AGAGGGAGAC GTGGTGCTTA AGATTAATGG CACGGTAACA 
GAGAATTTGT CCCTGATCGA TGCTAAGAAG CTGATCGAGA GGTCAAAGGG AAAGCTGAAG 
ATGGTGGTGC AGAGAGACGA GCGGGCGACG CTGCTGAACA TCCCCGATCT GGACGACAGC 
ATACCTTCTG CTAATGCCTC GGACAGAGAT GATATTTCAG ACATCCACTC CGTGGCCTCT 
GATCATTCCA ACCGATCCCA CGACCGGAAG AGGAGCAGCC GCTCTCGTTC TCCAGACCGC 
CGCTCCGAAC CCTCAGACCA CTCCAGACAC TCTCCACCAC AGATCAGCAA CGGCAGGGCC 
ACAGGGCCGA CGGCTCAGAG AACGCGGCCA GTTCACAGGA GCCGTGATGA AGACAGGATC 
TCCAAGGCCG GGCCGCTTCC TCTACCTGCT AAGATGGTGG AGGAGATGCC GAAAGAGCAG 
AGCGCAGCCA GAGAAACAGA GAAACAGCTC CCCCCTCTGC CAGAGCCCAA ACCTGTGTAC 
GCTCAGCCGG GCCAGCCGGA CGTGGACCTG CCTGTTAGTC CGGCAGATGC TCCTGTGCCC 
AGCGTCACAC ATGATGACAG CATCTTACGG CCGAGCATGA AGCTGGTGAA GTTCAAGAAG 
GGCGAGAGCG TGGGCTTGAG GCTCGCTGGG GGGAACGACG TTGGGATATT TGTGGCGGGA 
GTTCTGGAGG ACAGTCCTGC TGCTAAAGAG GGCCTGGAAG AGGGAGACCA GATTCTCAGG 
GTAAATAATG TGGATTTTGC AAATATCATC CGAGAGGAGG CAGTGCTGTT CCTGCTGGAT 
CTGCCCAAAG GAGAGGAGGT CACCATCCTG GCCCAGAAGA AGAAAGACGT GTACCGCCGC 
ATTGTAGAGT CTGACGTGGG AGACTCGTTC TACATCAGGA CTCATTTTGA ATATGAGAAG 
GAGTCGCCCT ATGGCCTGAG CTTCAATAAG GGGGAGGTCT TCAGAGTCGT CGACACCCTT 
TACAATGGCA AACTGGGCTC CTGGCTCGCC ATCCGCATCG GCAAAAACCA CCAGGAGGTG 
GAGCGGGGCA TCATCCCCAA CAAGAACAGG GCTGAGCAGT TATCAAGTGT CCAGTACACT 
CTCCCAAAGA CTCCAGGAGG GGATCGCGCC GACTTCTGGA GATTCAGAGG CCTCCGCAGC 
TCCAAACGAA ACCTGAGGAA GAGCAGAGAA GACCTGTCTG CTCAGCCGGT CCAAACCAAA 
TTCCCTGCTT ATGAAAGAGT GGTGCTTAGA GAAGCTGGTT TCTTGAGGCC AGTGGTCATT 
TTCGGACCGA TCGCGGATGT GGCTAGAGAG AAGCTGTCCA GAGAGGAGAC TGACCTCTTC 
GAACTTGCAA AGAGTGAACC CAGAGATGCG GGCACAGACC AGCGCAGCTC CGGAATCATC 
CGTCTCCATA CCATTAAACA GATCATTGAC CGAGACAAGC ACGCGGTGCT GGACATCACC 
CCTAATGCGG TGGACCGTCT GAACTACGCT CAGTGGTACC CCATCGTGGT GTTTCTGAAT 
CCAGACAGCA AACAGGGCGT CAAGAACATG AGGACCAGGC TCTGTCCCGA GTCCAGGAAG 
AGCGCCCGAA AGCTCTACGA GCGTGCTCTC AAACTGAGGA AGAACAACCA TCACCTTTTC 
ACCACCACTA TTAACTTGAA CAACATGAAT GACGGGTGGT ACGGTGCTTT GAAAGAGAGC 
ATCCAGCAGC AGCAGAACCA GTTGGTCTGG GTCTCAGAGG GCAAGGCCGA CGGCGCTCCT 
GACGATGACC TTGACATCCA TGACGACCGC CTCTCCTACC TCTCGGCTCC GGGCAGCGAG 
TACAGCATGT ACAGCACCGA CAGCCGCCAC ACGTCCGACT ATGAGGACAC CGACACGGAG 
GGTGGTGCCT ACACCGACCA GGAGCTGGAC GAGACCATGA ACGATGAGGT GGGCCTGCCT 
AGCGAGCCTG CCATCACACG CTCATCTGAG CCTGTGCGAG AGGACCCGCC AGTCATTCAG 
GACGCAGCTG GATACCCCAG CTACCAGCAT GCGGTACCGC AACCTGAAGC GGTGAGCCGC 
ATTGACCCGG CCGGGTTTAA GATGGCAGCG CCGCAGCAGA TGTATAAAAA GGATCTGTAC 
AGCATGGACG AGCCGGTGCG AGTGACCCAC GGCGTGAAGC CTCCCCCTCC CCAGCAGCAG 
CAGCAGCCTT TAGGCCCCCC AACCTCGCTC TCCTACAGCC ACCAGCCTGT CTACCAGGAC 
CAACAGCCAT ACCGCCAGTA CGACCACCCG CCTTATGGCT ATGACGGTGG CGGCTACGCA 
CAACCAAAGC CTCACAACTA CGACCCGCAC CTGCACTACG ACAACCGTGT GCCTCACTAT 
AACGAGCAGT GGCCCACGTA CGACCAGCAG CAGCAGCAGC AGACTTCCCC CCAGCCGCCA 
CCTGCGTCCG GCTTCCCTCA GGGCCACCAG CCTCCGCCTC CACCACCGAT GGGATATGAG 
CCACGCTCCC CATACGAGGA CGGACCTAAC AGGGATTTCA GCCCTCCTCA GTCCCAGTAT 
GACGGTGTGT CCCCAATGGG CTACGATCAC CGGCCGCGGC ACCCCAAACC TGCACCGGTC 
CGATATGAAG AGCCTCCACC TCCACCTCCT CCGGTGTCCT ACGATGCACG GTCACCTTTC 
GAACCCGAGC CGCATGCTTT CCCAGGCAAC ACACATCGCT CGCCAGACCC ACCGAAACAG 
TATTATGGAG ACGCCGCTAT GAGGCCCTTG TACAACCCTG GACCTCCGAA CAGAGTGTAC 
AAACATGAGC CGGTGAACTC TGAGCCTCCT GTACCGCCTC CCAAACCTGA ATCTGTCCTG 
TCTCCAGGAG AACCGCCCCA TTCTGCAGCT TCCAAACCAT TGCCGCCTCC TCCCAGAGAT 
GATGACGATG ATGATGACGA TGATGATGAC CCTGCCATGA AGCCCCAGTC TGTGCTCAAC 
AGGGTCAAAA TGTTCGAAAA CAAACGCTCT GTGTCGGTAG ACCGAGCCAA GGACGCACCT 
GACGTCACTG GAATTAGGCC CACAGATCTC CCCAAACCTG CGAGCACCCC TGGACCTGTG 
CTCAAAGCCA ACTCTCTCAG CAACCTGGAG CAGGACAAAC CCTCCTACAG AGCTCCAGAG 
CCGCAGAAAC CTCAGACGCG TGTTGGGGAC GATGTGGTTC GCTCCAACCA CTACGACCCT 
GATGATGATG AAGAGTACTA CAGAAAACAG CTCTCATTTT TCGACCGCCG CAGTTTCGAC 
AGTAAGGTCA TGAATCCACC CAGCGCTGGA GTAAACCGCT TCCATGAGCC GCCCAAACCA 
CCCCAGACAC AGATCGCTTA TCCTTTCGCT AGGACGGAGT CTGTGGAGAA GGTGAGTCCT 
GTGGACAAGA GATACGAGCC TCTGCCGCCA GTCAACCCTT CACCTGCTCC ATACAGCCAG 
AACACACCTG TCGCTCCGCC CACATCCCTG CCCAAACTCA CCAACATCGA GGTGAACTCT 
CTGCCAGATC CTCACAGCTC TCCCAAAGCC AAACCGGACC TGTCTGCTCT GAGAGCTCCG 
ACCCGGGACG AGCCCATCCA GAGCAGCTAC TTGCCCCCCA AGTCCACCAT CAATGGCATG 
GATGCTCCTC CCAAAACACT CGGCGTGCCG ACCAGCTACA ACCGCTACGT CCCCAAGCCT 
TACACCAGCT CGGCGCGACC CTTCGAGCGC AAGTTCGAGA GTCCGAAATT CAACCACAAC 
CTGCTTCCCA ACGACACGCA GTCCAAACCC AGTGTCAACA ACAACCTGAA GCCTCAGATC 
TCCCCACAAC CCCAGGACAC GGACAGCGGC GTCGACACCT TCACTCGCAC CATGGACAAC 
AGGCCAAAAT ATCAGCACAA CAACATCAAC GCCATTCCCA AAGCCGTCCC AGTCAGCCCC 
AGTGCACTGG ATGATGATGA AGAAGATGAG GGACACACAG TTGTGGCCAC GGCGCGGGGC 
ATCTTTAACT GTAATGGCGG GGTGTTGAGC TCTATAGAGA CGGGCGTCAG CATCATTATC 
CCACAGGGAG CCATTCCTGA TAGTGTGGAG CAGGAGATCT ACTTCAAAGT GTGCCGGGAC 
AACAGCATCC TGCCTCCTCT GGATAAGGAG AAAGGTGAAA CTCTGCTGAG TCCGCTGGTG 
ATGTGTGGTC CTCACGGCCT GAAGTTCCTG AAGCCTGTGG AGCTGCGCTT GCCACACTGT 
GCGTCTATGA CCCCTGATGG TTGGTCTTTT GCTCTAAAAT CCTCCGACTC CTCGTCGGGT 
GACCCCAAAT GCTGGCAAAA CAAGTGTCTA CCAGGAGACC CCAATTACCT GGTGGGCGCA 
AACTGTGTGT CTGTGCTGAT CGACCACTTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326408.1
CDS	110..6022
Translation	MLLSVFGGPVEEVLAGLLKLKMFGSGSKKWFIKKNKRQRTKVQRKPASGRRNKRVSRVLKRHKEVIVKQLDVSKVLPTLVYERVFTLGEYKEILGQDSSGKRAELFLDLLSQKEPAAFSAFCGVLEEVCPHLLTCFLLDGTDGSAGENKKQGQTKPAHSQQNALSKGQDTVCFPPMYTDPLFDTRVFPKHDKDKYGPAVYYPKFNAYIPNPDKVSVLDESEGSGHGKPSLRRIKGRIHRSKSLDSIDLLDSNSAAMDETVIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGLLQENDRVVMVNAVSMDNVDHAYAVQQLRKSGKNAKITIRRKRKVQIPSGRPGERETMSEHDEDDDSYEDEIYESRSARSGPSAYSGGGGGGGGGGASGRRSGRGDRLSGRRERDRERSGSRERSLSPRSDRHSVSSNLPPRPAKVTLVKSRKNEACMASGSSQKVTSAEYGLRLASHIFVKDISPESLAARDGNIQEGDVVLKINGTVTENLSLIDAKKLIERSKGKLKMVVQRDERATLLNIPDLDDSIPSANASDRDDISDIHSVASDHSNRSHDRKRSSRSRSPDRRSEPSDHSRHSPPQISNGRATGPTAQRTRPVHRSRDEDRISKAGPLPLPAKMVEEMPKEQSAARETEKQLPPLPEPKPVYAQPGQPDVDLPVSPADAPVPSVTHDDSILRPSMKLVKFKKGESVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFANIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHQEVERGIIPNKNRAEQLSSVQYTLPKTPGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVVIFGPIADVAREKLSREETDLFELAKSEPRDAGTDQRSSGIIRLHTIKQIIDRDKHAVLDITPNAVDRLNYAQWYPIVVFLNPDSKQGVKNMRTRLCPESRKSARKLYERALKLRKNNHHLFTTTINLNNMNDGWYGALKESIQQQQNQLVWVSEGKADGAPDDDLDIHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETMNDEVGLPSEPAITRSSEPVREDPPVIQDAAGYPSYQHAVPQPEAVSRIDPAGFKMAAPQQMYKKDLYSMDEPVRVTHGVKPPPPQQQQQPLGPPTSLSYSHQPVYQDQQPYRQYDHPPYGYDGGGYAQPKPHNYDPHLHYDNRVPHYNEQWPTYDQQQQQQTSPQPPPASGFPQGHQPPPPPPMGYEPRSPYEDGPNRDFSPPQSQYDGVSPMGYDHRPRHPKPAPVRYEEPPPPPPPVSYDARSPFEPEPHAFPGNTHRSPDPPKQYYGDAAMRPLYNPGPPNRVYKHEPVNSEPPVPPPKPESVLSPGEPPHSAASKPLPPPPRDDDDDDDDDDDPAMKPQSVLNRVKMFENKRSVSVDRAKDAPDVTGIRPTDLPKPASTPGPVLKANSLSNLEQDKPSYRAPEPQKPQTRVGDDVVRSNHYDPDDDEEYYRKQLSFFDRRSFDSKVMNPPSAGVNRFHEPPKPPQTQIAYPFARTESVEKVSPVDKRYEPLPPVNPSPAPYSQNTPVAPPTSLPKLTNIEVNSLPDPHSSPKAKPDLSALRAPTRDEPIQSSYLPPKSTINGMDAPPKTLGVPTSYNRYVPKPYTSSARPFERKFESPKFNHNLLPNDTQSKPSVNNNLKPQISPQPQDTDSGVDTFTRTMDNRPKYQHNNINAIPKAVPVSPSALDDDEEDEGHTVVATARGIFNCNGGVLSSIETGVSIIIPQGAIPDSVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKCWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_021470733.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tight junction protein 1b (tjp1b), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470733.1

ORF Insert Sequence:

ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
TCCCGCAAGA ATGAAGCATG TATGGCGTCA GGAAGTTCTC AGAAGGTGAC ATCAGCAGAA 
TACGGGCTTC GCCTGGCCAG CCACATCTTC GTGAAGGACA TTTCCCCCGA GAGCCTGGCA 
GCCCGAGACG GAAACATCCA AGAGGGAGAC GTGGTGCTTA AGATTAATGG CACGGTAACA 
GAGAATTTGT CCCTGATCGA TGCTAAGAAG CTGATCGAGA GGTCAAAGGG AAAGCTGAAG 
ATGGTGGTGC AGAGAGACGA GCGGGCGACG CTGCTGAACA TCCCCGATCT GGACGACAGC 
ATACCTTCTG CTAATGCCTC GGACAGAGAT GATATTTCAG ACATCCACTC CGTGGCCTCT 
GATCATTCCA ACCGATCCCA CGACCGGAAG AGGAGCAGCC GCTCTCGTTC TCCAGACCGC 
CGCTCCGAAC CCTCAGACCA CTCCAGACAC TCTCCACCAC AGATCAGCAA CGGCAGGGCC 
ACAGGGCCGA CGGCTCAGAG AACGCGGCCA GTTCACAGGA GCCGTGATGA AGACAGGATC 
TCCAAGGCCG GGCCGCTTCC TCTACCTGCT AAGATGGTGG AGGAGATGCC GAAAGAGCAG 
AGCGCAGCCA GAGAAACAGA GAAACAGCTC CCCCCTCTGC CAGAGCCCAA ACCTGTGTAC 
GCTCAGCCGG GCCAGCCGGA CGTGGACCTG CCTGTTAGTC CGGCAGATGC TCCTGTGCCC 
AGCGTCACAC ATGATGACAG CATCTTACGG CCGAGCATGA AGCTGGTGAA GTTCAAGAAG 
GGCGAGAGCG TGGGCTTGAG GCTCGCTGGG GGGAACGACG TTGGGATATT TGTGGCGGGA 
GTTCTGGAGG ACAGTCCTGC TGCTAAAGAG GGCCTGGAAG AGGGAGACCA GATTCTCAGG 
GTAAATAATG TGGATTTTGC AAATATCATC CGAGAGGAGG CAGTGCTGTT CCTGCTGGAT 
CTGCCCAAAG GAGAGGAGGT CACCATCCTG GCCCAGAAGA AGAAAGACGT GTACCGCCGC 
ATTGTAGAGT CTGACGTGGG AGACTCGTTC TACATCAGGA CTCATTTTGA ATATGAGAAG 
GAGTCGCCCT ATGGCCTGAG CTTCAATAAG GGGGAGGTCT TCAGAGTCGT CGACACCCTT 
TACAATGGCA AACTGGGCTC CTGGCTCGCC ATCCGCATCG GCAAAAACCA CCAGGAGGTG 
GAGCGGGGCA TCATCCCCAA CAAGAACAGG GCTGAGCAGT TATCAAGTGT CCAGTACACT 
CTCCCAAAGA CTCCAGGAGG GGATCGCGCC GACTTCTGGA GATTCAGAGG CCTCCGCAGC 
TCCAAACGAA ACCTGAGGAA GAGCAGAGAA GACCTGTCTG CTCAGCCGGT CCAAACCAAA 
TTCCCTGCTT ATGAAAGAGT GGTGCTTAGA GAAGCTGGTT TCTTGAGGCC AGTGGTCATT 
TTCGGACCGA TCGCGGATGT GGCTAGAGAG AAGCTGTCCA GAGAGGAGAC TGACCTCTTC 
GAACTTGCAA AGAGTGAACC CAGAGATGCG GGCACAGACC AGCGCAGCTC CGGAATCATC 
CGTCTCCATA CCATTAAACA GATCATTGAC CGAGACAAGC ACGCGGTGCT GGACATCACC 
CCTAATGCGG TGGACCGTCT GAACTACGCT CAGTGGTACC CCATCGTGGT GTTTCTGAAT 
CCAGACAGCA AACAGGGCGT CAAGAACATG AGGACCAGGC TCTGTCCCGA GTCCAGGAAG 
AGCGCCCGAA AGCTCTACGA GCGTGCTCTC AAACTGAGGA AGAACAACCA TCACCTTTTC 
ACCACCACTA TTAACTTGAA CAACATGAAT GACGGGTGGT ACGGTGCTTT GAAAGAGAGC 
ATCCAGCAGC AGCAGAACCA GTTGGTCTGG GTCTCAGAGG GCAAGGCCGA CGGCGCTCCT 
GACGATGACC TTGACATCCA TGACGACCGC CTCTCCTACC TCTCGGCTCC GGGCAGCGAG 
TACAGCATGT ACAGCACCGA CAGCCGCCAC ACGTCCGACT ATGAGGACAC CGACACGGAG 
GGTGGTGCCT ACACCGACCA GGAGCTGGAC GAGACCATGA ACGATGAGGT GGGCCTGCCT 
AGCGAGCCTG CCATCACACG CTCATCTGAG CCTGTGCGAG AGGACCCGCC AGTCATTCAG 
GACGCAGCTG GATACCCCAG CTACCAGCAT GCGGTACCGC AACCTGAAGC GGTGAGCCGC 
ATTGACCCGG CCGGGTTTAA GATGGCAGCG CCGCAGCAGA TGTATAAAAA GGATCTGTAC 
AGCATGGACG AGCCGGTGCG AGTGACCCAC GGCGTGAAGC CTCCCCCTCC CCAGCAGCAG 
CAGCAGCCTT TAGGCCCCCC AACCTCGCTC TCCTACAGCC ACCAGCCTGT CTACCAGGAC 
CAACAGCCAT ACCGCCAGTA CGACCACCCG CCTTATGGCT ATGACGGTGG CGGCTACGCA 
CAACCAAAGC CTCACAACTA CGACCCGCAC CTGCACTACG ACAACCGTGT GCCTCACTAT 
AACGAGCAGT GGCCCACGTA CGACCAGCAG CAGCAGCAGC AGACTTCCCC CCAGCCGCCA 
CCTGCGTCCG GCTTCCCTCA GGGCCACCAG CCTCCGCCTC CACCACCGAT GGGATATGAG 
CCACGCTCCC CATACGAGGA CGGACCTAAC AGGGATTTCA GCCCTCCTCA GTCCCAGTAT 
GACGGTGTGT CCCCAATGGG CTACGATCAC CGGCCGCGGC ACCCCAAACC TGCACCGGTC 
CGATATGAAG AGCCTCCACC TCCACCTCCT CCGGTGTCCT ACGATGCACG GTCACCTTTC 
GAACCCGAGC CGCATGCTTT CCCAGGCAAC ACACATCGCT CGCCAGACCC ACCGAAACAG 
TATTATGGAG ACGCCGCTAT GAGGCCCTTG TACAACCCTG GACCTCCGAA CAGAGTGTAC 
AAACATGAGC CGGTGAACTC TGAGCCTCCT GTACCGCCTC CCAAACCTGA ATCTGTCCTG 
TCTCCAGGAG AACCGCCCCA TTCTGCAGCT TCCAAACCAT TGCCGCCTCC TCCCAGAGAT 
GATGACGATG ATGATGACGA TGATGATGAC CCTGCCATGA AGCCCCAGTC TGTGCTCAAC 
AGGGTCAAAA TGTTCGAAAA CAAACGCTCT GTGTCGGTAG ACCGAGCCAA GGACGCACCT 
GACGTCACTG GAATTAGGCC CACAGATCTC CCCAAACCTG CGAGCACCCC TGGACCTGTG 
CTCAAAGCCA ACTCTCTCAG CAACCTGGAG CAGGACAAAC CCTCCTACAG AGCTCCAGAG 
CCGCAGAAAC CTCAGACGCG TGTTGGGGAC GATGTGGTTC GCTCCAACCA CTACGACCCT 
GATGATGATG AAGAGTACTA CAGAAAACAG CTCTCATTTT TCGACCGCCG CAGTTTCGAC 
AGTAAGGTCA TGAATCCACC CAGCGCTGGA GTAAACCGCT TCCATGAGCC GCCCAAACCA 
CCCCAGACAC AGATCGCTTA TCCTTTCGCT AGGACGGAGT CTGTGGAGAA GGTGAGTCCT 
GTGGACAAGA GATACGAGCC TCTGCCGCCA GTCAACCCTT CACCTGCTCC ATACAGCCAG 
AACACACCTG TCGCTCCGCC CACATCCCTG CCCAAACTCA CCAACATCGA GGTGAACTCT 
CTGCCAGATC CTCACAGCTC TCCCAAAGCC AAACCGGACC TGTCTGCTCT GAGAGCTCCG 
ACCCGGGACG AGCCCATCCA GAGCAGCTAC TTGCCCCCCA AGTCCACCAT CAATGGCATG 
GATGCTCCTC CCAAAACACT CGGCGTGCCG ACCAGCTACA ACCGCTACGT CCCCAAGCCT 
TACACCAGCT CGGCGCGACC CTTCGAGCGC AAGTTCGAGA GTCCGAAATT CAACCACAAC 
CTGCTTCCCA ACGACACGCA GTCCAAACCC AGTGTCAACA ACAACCTGAA GCCTCAGATC 
TCCCCACAAC CCCAGGACAC GGACAGCGGC GTCGACACCT TCACTCGCAC CATGGACAAC 
AGGCCAAAAT ATCAGCACAA CAACATCAAC GCCATTCCCA AAGCCGTCCC AGTCAGCCCC 
AGTGCACTGG ATGATGATGA AGAAGATGAG GGACACACAG TTGTGGCCAC GGCGCGGGGC 
ATCTTTAACT GTAATGGCGG GGTGTTGAGC TCTATAGAGA CGGGCGTCAG CATCATTATC 
CCACAGGGAG CCATTCCTGA TAGTGTGGAG CAGGAGATCT ACTTCAAAGT GTGCCGGGAC 
AACAGCATCC TGCCTCCTCT GGATAAGGAG AAAGGTGAAA CTCTGCTGAG TCCGCTGGTG 
ATGTGTGGTC CTCACGGCCT GAAGTTCCTG AAGCCTGTGG AGCTGCGCTT GCCACACTGT 
GCGTCTATGA CCCCTGATGG TTGGTCTTTT GCTCTAAAAT CCTCCGACTC CTCGTCGGGT 
GACCCCAAAT GCTGGCAAAA CAAGTGTCTA CCAGGAGACC CCAATTACCT GGTGGGCGCA 
AACTGTGTGT CTGTGCTGAT CGACCACTTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp1b ( XM_009298137.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296412.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X16, mRNA.

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CloneID	ODa44627
Clone ID Related Accession (Same CDS sequence)	XM_009298137.3 , XM_009298137.2
Accession Version	XM_009298137.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5817bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tight junction protein ZO-1 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009298137.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
TCCCGCAAGA ATGAAGATAT TTCAGACATC CACTCCGTGG CCTCTGATCA TTCCAACCGA 
TCCCACGACC GGAAGAGGAG CAGCCGCTCT CGTTCTCCAG ACCGCCGCTC CGAACCCTCA 
GACCACTCCA GACACTCTCC ACCACAGATC AGCAACGGCA GGGCCACAGG GCCGACGGCT 
CAGAGAACGC GGCCAGTTCA CAGGAGCCGT GATGAAGACA GGATCTCCAA GGCCGGGCCG 
CTTCCTCTAC CTGCTAAGAT GGTGGAGGAG ATGCCGAAAG AGCAGAGCGC AGCCAGAGAA 
ACAGAGAAAC AGCTCCCCCC TCTGCCAGAG CCCAAACCTG TGTACGCTCA GCCGGGCCAG 
CCGGACGTGG ACCTGCCTGT TAGTCCGGCA GATGCTCCTG TGCCCAGCGT CACACATGAT 
GACAGCATCT TACGGCCGAG CATGAAGCTG GTGAAGTTCA AGAAGGGCGA GAGCGTGGGC 
TTGAGGCTCG CTGGGGGGAA CGACGTTGGG ATATTTGTGG CGGGAGTTCT GGAGGACAGT 
CCTGCTGCTA AAGAGGGCCT GGAAGAGGGA GACCAGATTC TCAGGGTAAA TAATGTGGAT 
TTTGCAAATA TCATCCGAGA GGAGGCAGTG CTGTTCCTGC TGGATCTGCC CAAAGGAGAG 
GAGGTCACCA TCCTGGCCCA GAAGAAGAAA GACGTGTACC GCCGCATTGT AGAGTCTGAC 
GTGGGAGACT CGTTCTACAT CAGGACTCAT TTTGAATATG AGAAGGAGTC GCCCTATGGC 
CTGAGCTTCA ATAAGGGGGA GGTCTTCAGA GTCGTCGACA CCCTTTACAA TGGCAAACTG 
GGCTCCTGGC TCGCCATCCG CATCGGCAAA AACCACCAGG AGGTGGAGCG GGGCATCATC 
CCCAACAAGA ACAGGGCTGA GCAGTTATCA AGTGTCCAGT ACACTCTCCC AAAGACTCCA 
GGAGGGGATC GCGCCGACTT CTGGAGATTC AGAGGCCTCC GCAGCTCCAA ACGAAACCTG 
AGGAAGAGCA GAGAAGACCT GTCTGCTCAG CCGGTCCAAA CCAAATTCCC TGCTTATGAA 
AGAGTGGTGC TTAGAGAAGC TGGTTTCTTG AGGCCAGTGG TCATTTTCGG ACCGATCGCG 
GATGTGGCTA GAGAGAAGCT GTCCAGAGAG GAGACTGACC TCTTCGAACT TGCAAAGAGT 
GAACCCAGAG ATGCGGGCAC AGACCAGCGC AGCTCCGGAA TCATCCGTCT CCATACCATT 
AAACAGATCA TTGACCGAGA CAAGCACGCG GTGCTGGACA TCACCCCTAA TGCGGTGGAC 
CGTCTGAACT ACGCTCAGTG GTACCCCATC GTGGTGTTTC TGAATCCAGA CAGCAAACAG 
GGCGTCAAGA ACATGAGGAC CAGGCTCTGT CCCGAGTCCA GGAAGAGCGC CCGAAAGCTC 
TACGAGCGTG CTCTCAAACT GAGGAAGAAC AACCATCACC TTTTCACCAC CACTATTAAC 
TTGAACAACA TGAATGACGG GTGGTACGGT GCTTTGAAAG AGAGCATCCA GCAGCAGCAG 
AACCAGTTGG TCTGGGTCTC AGAGGGCAAG GCCGACGGCG CTCCTGACGA TGACCTTGAC 
ATCCATGACG ACCGCCTCTC CTACCTCTCG GCTCCGGGCA GCGAGTACAG CATGTACAGC 
ACCGACAGCC GCCACACGTC CGACTATGAG GACACCGACA CGGAGGGTGG TGCCTACACC 
GACCAGGAGC TGGACGAGAC CATGAACGAT GAGGTGGGCC TGCCTAGCGA GCCTGCCATC 
ACACGCTCAT CTGAGCCTGT GCGAGAGGAC CCGCCAGTCA TTCAGGACGC AGCTGGATAC 
CCCAGCTACC AGCATGCGGT ACCGCAACCT GAAGCGGTGA GCCGCATTGA CCCGGCCGGG 
TTTAAGATGG CAGCGCCGCA GCAGCAAGCT GAGGCTGCTC TGGCCTCGCC GCCCCCTCCC 
TCTGCAGCGG TCCAGCCCAC CGCAACACTA GCGGGTACGA ACCCCGAGGA GCCGCCTGGC 
ACCCCTCAGG CCGACTCCCT TAACAGCCCC GTCCCTGTTC CCGACCCCCA GCCCGAACCT 
GAGCTCGCTC AGCCCCCAAC ACACGACCCC CACCAGTCGC TTCCTCCTGG CCCAGATCCA 
AAGATGTATA AAAAGGATCT GTACAGCATG GACGAGCCGG TGCGAGTGAC CCACGGCGTG 
AAGCCTCCCC CTCCCCAGCA GCAGCAGCAG CCTTTAGGCC CCCCAACCTC GCTCTCCTAC 
AGCCACCAGC CTGTCTACCA GGACCAACAG CCATACCGCC AGTACGACCA CCCGCCTTAT 
GGCTATGACG GTGGCGGCTA CGCACAACCA AAGCCTCACA ACTACGACCC GCACCTGCAC 
TACGACAACC GTGTGCCTCA CTATAACGAG CAGTGGCCCA CGTACGACCA GCAGCAGCAG 
CAGCAGACTT CCCCCCAGCC GCCACCTGCG TCCGGCTTCC CTCAGGGCCA CCAGCCTCCG 
CCTCCACCAC CGATGGGATA TGAGCCACGC TCCCCATACG AGGACGGACC TAACAGGGAT 
TTCAGCCCTC CTCAGTCCCA GTATGACGGT GTGTCCCCAA TGGGCTACGA TCACCGGCCG 
CGGCACCCCA AACCTGCACC GGTCCGATAT GAAGAGCCTC CACCTCCACC TCCTCCGGTG 
TCCTACGATG CACGGTCACC TTTCGAACCC GAGCCGCATG CTTTCCCAGG CAACACACAT 
CGCTCGCCAG ACCCACCGAA ACAGTATTAT GGAGACGCCG CTATGAGGCC CTTGTACAAC 
CCTGGACCTC CGAACAGAGT GTACAAACAT GAGCCGGTGA ACTCTGAGCC TCCTGTACCG 
CCTCCCAAAC CTGAATCTGT CCTGTCTCCA GGAGAACCGC CCCATTCTGC AGCTTCCAAA 
CCATTGCCGC CTCCTCCCAG AGATGATGAC GATGATGATG ACGATGATGA TGACCCTGCC 
ATGAAGCCCC AGTCTGTGCT CAACAGGGTC AAAATGTTCG AAAACAAACG CTCTGTGTCG 
GTAGACCGAG CCAAGGACGC ACCTGACGTC ACTGGAATTA GGCCCACAGA TCTCCCCAAA 
CCTGCGAGCA CCCCTGGACC TGTGCTCAAA GCCAACTCTC TCAGCAACCT GGAGCAGGAC 
AAACCCTCCT ACAGAGCTCC AGAGCCGCAG AAACCTCAGA CGCGTGTTGG GGACGATGTG 
GTTCGCTCCA ACCACTACGA CCCTGATGAT GATGAAGAGT ACTACAGAAA ACAGCTCTCA 
TTTTTCGACC GCCGCAGTTT CGACAGTAAG GTCATGAATC CACCCAGCGC TGGAGTAAAC 
CGCTTCCATG AGCCGCCCAA ACCACCCCAG ACACAGATCG CTTATCCTTT CGCTAGGACG 
GAGTCTGTGG AGAAGGTGAG TCCTGTGGAC AAGAGATACG AGCCTCTGCC GCCAGTCAAC 
CCTTCACCTG CTCCATACAG CCAGAACACA CCTGTCGCTC CGCCCACATC CCTGCCCAAA 
CTCACCAACA TCGAGGTGAA CTCTCTGCCA GATCCTCACA GCTCTCCCAA AGCCAAACCG 
GACCTGTCTG CTCTGAGAGC TCCGACCCGG GACGAGCCCA TCCAGAGCAG CTACTTGCCC 
CCCAAGTCCA CCATCAATGG CATGGATGCT CCTCCCAAAA CACTCGGCGT GCCGACCAGC 
TACAACCGCT ACGTCCCCAA GCCTTACACC AGCTCGGCGC GACCCTTCGA GCGCAAGTTC 
GAGAGTCCGA AATTCAACCA CAACCTGCTT CCCAACGACA CGCAGTCCAA ACCCAGTGTC 
AACAACAACC TGAAGCCTCA GATCTCCCCA CAACCCCAGG ACACGGACAG CGGCGTCGAC 
ACCTTCACTC GCACCATGGA CAACAGGCCA AAATATCAGC ACAACAACAT CAACGCCATT 
CCCAAAGCCG TCCCAGTCAG CCCCAGTGCA CTGGATGATG ATGAAGAAGA TGAGGGACAC 
ACAGTTGTGG CCACGGCGCG GGGCATCTTT AACTGTAATG GCGGGGTGTT GAGCTCTATA 
GAGACGGGCG TCAGCATCAT TATCCCACAG GGAGCCATTC CTGATAGTGT GGAGCAGGAG 
ATCTACTTCA AAGTGTGCCG GGACAACAGC ATCCTGCCTC CTCTGGATAA GGAGAAAGGT 
GAAACTCTGC TGAGTCCGCT GGTGATGTGT GGTCCTCACG GCCTGAAGTT CCTGAAGCCT 
GTGGAGCTGC GCTTGCCACA CTGTGCGTCT ATGACCCCTG ATGGTTGGTC TTTTGCTCTA 
AAATCCTCCG ACTCCTCGTC GGGTGACCCC AAATGCTGGC AAAACAAGTG TCTACCAGGA 
GACCCCAATT ACCTGGTGGG CGCAAACTGT GTGTCTGTGC TGATCGACCA CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296412.1
CDS	110..5926
Translation	MLLSVFGGPVEEVLAGLLKLKMFGSGSKKWFIKKNKRQRTKVQRKPASGRRNKRVSRVLKRHKEVIVKQLDVSKVLPTLVYERVFTLGEYKEILGQDSSGKRAELFLDLLSQKEPAAFSAFCGVLEEVCPHLLTCFLLDGTDGSAGENKKQGQTKPAHSQQNALSKGQDTVCFPPMYTDPLFDTRVFPKHDKDKYGPAVYYPKFNAYIPNPDKVSVLDESEGSGHGKPSLRRIKGRIHRSKSLDSIDLLDSNSAAMDETVIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGLLQENDRVVMVNAVSMDNVDHAYAVQQLRKSGKNAKITIRRKRKVQIPSGRPGERETMSEHDEDDDSYEDEIYESRSARSGPSAYSGGGGGGGGGGASGRRSGRGDRLSGRRERDRERSGSRERSLSPRSDRHSVSSNLPPRPAKVTLVKSRKNEDISDIHSVASDHSNRSHDRKRSSRSRSPDRRSEPSDHSRHSPPQISNGRATGPTAQRTRPVHRSRDEDRISKAGPLPLPAKMVEEMPKEQSAARETEKQLPPLPEPKPVYAQPGQPDVDLPVSPADAPVPSVTHDDSILRPSMKLVKFKKGESVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFANIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHQEVERGIIPNKNRAEQLSSVQYTLPKTPGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVVIFGPIADVAREKLSREETDLFELAKSEPRDAGTDQRSSGIIRLHTIKQIIDRDKHAVLDITPNAVDRLNYAQWYPIVVFLNPDSKQGVKNMRTRLCPESRKSARKLYERALKLRKNNHHLFTTTINLNNMNDGWYGALKESIQQQQNQLVWVSEGKADGAPDDDLDIHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETMNDEVGLPSEPAITRSSEPVREDPPVIQDAAGYPSYQHAVPQPEAVSRIDPAGFKMAAPQQQAEAALASPPPPSAAVQPTATLAGTNPEEPPGTPQADSLNSPVPVPDPQPEPELAQPPTHDPHQSLPPGPDPKMYKKDLYSMDEPVRVTHGVKPPPPQQQQQPLGPPTSLSYSHQPVYQDQQPYRQYDHPPYGYDGGGYAQPKPHNYDPHLHYDNRVPHYNEQWPTYDQQQQQQTSPQPPPASGFPQGHQPPPPPPMGYEPRSPYEDGPNRDFSPPQSQYDGVSPMGYDHRPRHPKPAPVRYEEPPPPPPPVSYDARSPFEPEPHAFPGNTHRSPDPPKQYYGDAAMRPLYNPGPPNRVYKHEPVNSEPPVPPPKPESVLSPGEPPHSAASKPLPPPPRDDDDDDDDDDDPAMKPQSVLNRVKMFENKRSVSVDRAKDAPDVTGIRPTDLPKPASTPGPVLKANSLSNLEQDKPSYRAPEPQKPQTRVGDDVVRSNHYDPDDDEEYYRKQLSFFDRRSFDSKVMNPPSAGVNRFHEPPKPPQTQIAYPFARTESVEKVSPVDKRYEPLPPVNPSPAPYSQNTPVAPPTSLPKLTNIEVNSLPDPHSSPKAKPDLSALRAPTRDEPIQSSYLPPKSTINGMDAPPKTLGVPTSYNRYVPKPYTSSARPFERKFESPKFNHNLLPNDTQSKPSVNNNLKPQISPQPQDTDSGVDTFTRTMDNRPKYQHNNINAIPKAVPVSPSALDDDEEDEGHTVVATARGIFNCNGGVLSSIETGVSIIIPQGAIPDSVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKCWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_009298137.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tight junction protein 1b (tjp1b), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298137.3

ORF Insert Sequence:

ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
TCCCGCAAGA ATGAAGATAT TTCAGACATC CACTCCGTGG CCTCTGATCA TTCCAACCGA 
TCCCACGACC GGAAGAGGAG CAGCCGCTCT CGTTCTCCAG ACCGCCGCTC CGAACCCTCA 
GACCACTCCA GACACTCTCC ACCACAGATC AGCAACGGCA GGGCCACAGG GCCGACGGCT 
CAGAGAACGC GGCCAGTTCA CAGGAGCCGT GATGAAGACA GGATCTCCAA GGCCGGGCCG 
CTTCCTCTAC CTGCTAAGAT GGTGGAGGAG ATGCCGAAAG AGCAGAGCGC AGCCAGAGAA 
ACAGAGAAAC AGCTCCCCCC TCTGCCAGAG CCCAAACCTG TGTACGCTCA GCCGGGCCAG 
CCGGACGTGG ACCTGCCTGT TAGTCCGGCA GATGCTCCTG TGCCCAGCGT CACACATGAT 
GACAGCATCT TACGGCCGAG CATGAAGCTG GTGAAGTTCA AGAAGGGCGA GAGCGTGGGC 
TTGAGGCTCG CTGGGGGGAA CGACGTTGGG ATATTTGTGG CGGGAGTTCT GGAGGACAGT 
CCTGCTGCTA AAGAGGGCCT GGAAGAGGGA GACCAGATTC TCAGGGTAAA TAATGTGGAT 
TTTGCAAATA TCATCCGAGA GGAGGCAGTG CTGTTCCTGC TGGATCTGCC CAAAGGAGAG 
GAGGTCACCA TCCTGGCCCA GAAGAAGAAA GACGTGTACC GCCGCATTGT AGAGTCTGAC 
GTGGGAGACT CGTTCTACAT CAGGACTCAT TTTGAATATG AGAAGGAGTC GCCCTATGGC 
CTGAGCTTCA ATAAGGGGGA GGTCTTCAGA GTCGTCGACA CCCTTTACAA TGGCAAACTG 
GGCTCCTGGC TCGCCATCCG CATCGGCAAA AACCACCAGG AGGTGGAGCG GGGCATCATC 
CCCAACAAGA ACAGGGCTGA GCAGTTATCA AGTGTCCAGT ACACTCTCCC AAAGACTCCA 
GGAGGGGATC GCGCCGACTT CTGGAGATTC AGAGGCCTCC GCAGCTCCAA ACGAAACCTG 
AGGAAGAGCA GAGAAGACCT GTCTGCTCAG CCGGTCCAAA CCAAATTCCC TGCTTATGAA 
AGAGTGGTGC TTAGAGAAGC TGGTTTCTTG AGGCCAGTGG TCATTTTCGG ACCGATCGCG 
GATGTGGCTA GAGAGAAGCT GTCCAGAGAG GAGACTGACC TCTTCGAACT TGCAAAGAGT 
GAACCCAGAG ATGCGGGCAC AGACCAGCGC AGCTCCGGAA TCATCCGTCT CCATACCATT 
AAACAGATCA TTGACCGAGA CAAGCACGCG GTGCTGGACA TCACCCCTAA TGCGGTGGAC 
CGTCTGAACT ACGCTCAGTG GTACCCCATC GTGGTGTTTC TGAATCCAGA CAGCAAACAG 
GGCGTCAAGA ACATGAGGAC CAGGCTCTGT CCCGAGTCCA GGAAGAGCGC CCGAAAGCTC 
TACGAGCGTG CTCTCAAACT GAGGAAGAAC AACCATCACC TTTTCACCAC CACTATTAAC 
TTGAACAACA TGAATGACGG GTGGTACGGT GCTTTGAAAG AGAGCATCCA GCAGCAGCAG 
AACCAGTTGG TCTGGGTCTC AGAGGGCAAG GCCGACGGCG CTCCTGACGA TGACCTTGAC 
ATCCATGACG ACCGCCTCTC CTACCTCTCG GCTCCGGGCA GCGAGTACAG CATGTACAGC 
ACCGACAGCC GCCACACGTC CGACTATGAG GACACCGACA CGGAGGGTGG TGCCTACACC 
GACCAGGAGC TGGACGAGAC CATGAACGAT GAGGTGGGCC TGCCTAGCGA GCCTGCCATC 
ACACGCTCAT CTGAGCCTGT GCGAGAGGAC CCGCCAGTCA TTCAGGACGC AGCTGGATAC 
CCCAGCTACC AGCATGCGGT ACCGCAACCT GAAGCGGTGA GCCGCATTGA CCCGGCCGGG 
TTTAAGATGG CAGCGCCGCA GCAGCAAGCT GAGGCTGCTC TGGCCTCGCC GCCCCCTCCC 
TCTGCAGCGG TCCAGCCCAC CGCAACACTA GCGGGTACGA ACCCCGAGGA GCCGCCTGGC 
ACCCCTCAGG CCGACTCCCT TAACAGCCCC GTCCCTGTTC CCGACCCCCA GCCCGAACCT 
GAGCTCGCTC AGCCCCCAAC ACACGACCCC CACCAGTCGC TTCCTCCTGG CCCAGATCCA 
AAGATGTATA AAAAGGATCT GTACAGCATG GACGAGCCGG TGCGAGTGAC CCACGGCGTG 
AAGCCTCCCC CTCCCCAGCA GCAGCAGCAG CCTTTAGGCC CCCCAACCTC GCTCTCCTAC 
AGCCACCAGC CTGTCTACCA GGACCAACAG CCATACCGCC AGTACGACCA CCCGCCTTAT 
GGCTATGACG GTGGCGGCTA CGCACAACCA AAGCCTCACA ACTACGACCC GCACCTGCAC 
TACGACAACC GTGTGCCTCA CTATAACGAG CAGTGGCCCA CGTACGACCA GCAGCAGCAG 
CAGCAGACTT CCCCCCAGCC GCCACCTGCG TCCGGCTTCC CTCAGGGCCA CCAGCCTCCG 
CCTCCACCAC CGATGGGATA TGAGCCACGC TCCCCATACG AGGACGGACC TAACAGGGAT 
TTCAGCCCTC CTCAGTCCCA GTATGACGGT GTGTCCCCAA TGGGCTACGA TCACCGGCCG 
CGGCACCCCA AACCTGCACC GGTCCGATAT GAAGAGCCTC CACCTCCACC TCCTCCGGTG 
TCCTACGATG CACGGTCACC TTTCGAACCC GAGCCGCATG CTTTCCCAGG CAACACACAT 
CGCTCGCCAG ACCCACCGAA ACAGTATTAT GGAGACGCCG CTATGAGGCC CTTGTACAAC 
CCTGGACCTC CGAACAGAGT GTACAAACAT GAGCCGGTGA ACTCTGAGCC TCCTGTACCG 
CCTCCCAAAC CTGAATCTGT CCTGTCTCCA GGAGAACCGC CCCATTCTGC AGCTTCCAAA 
CCATTGCCGC CTCCTCCCAG AGATGATGAC GATGATGATG ACGATGATGA TGACCCTGCC 
ATGAAGCCCC AGTCTGTGCT CAACAGGGTC AAAATGTTCG AAAACAAACG CTCTGTGTCG 
GTAGACCGAG CCAAGGACGC ACCTGACGTC ACTGGAATTA GGCCCACAGA TCTCCCCAAA 
CCTGCGAGCA CCCCTGGACC TGTGCTCAAA GCCAACTCTC TCAGCAACCT GGAGCAGGAC 
AAACCCTCCT ACAGAGCTCC AGAGCCGCAG AAACCTCAGA CGCGTGTTGG GGACGATGTG 
GTTCGCTCCA ACCACTACGA CCCTGATGAT GATGAAGAGT ACTACAGAAA ACAGCTCTCA 
TTTTTCGACC GCCGCAGTTT CGACAGTAAG GTCATGAATC CACCCAGCGC TGGAGTAAAC 
CGCTTCCATG AGCCGCCCAA ACCACCCCAG ACACAGATCG CTTATCCTTT CGCTAGGACG 
GAGTCTGTGG AGAAGGTGAG TCCTGTGGAC AAGAGATACG AGCCTCTGCC GCCAGTCAAC 
CCTTCACCTG CTCCATACAG CCAGAACACA CCTGTCGCTC CGCCCACATC CCTGCCCAAA 
CTCACCAACA TCGAGGTGAA CTCTCTGCCA GATCCTCACA GCTCTCCCAA AGCCAAACCG 
GACCTGTCTG CTCTGAGAGC TCCGACCCGG GACGAGCCCA TCCAGAGCAG CTACTTGCCC 
CCCAAGTCCA CCATCAATGG CATGGATGCT CCTCCCAAAA CACTCGGCGT GCCGACCAGC 
TACAACCGCT ACGTCCCCAA GCCTTACACC AGCTCGGCGC GACCCTTCGA GCGCAAGTTC 
GAGAGTCCGA AATTCAACCA CAACCTGCTT CCCAACGACA CGCAGTCCAA ACCCAGTGTC 
AACAACAACC TGAAGCCTCA GATCTCCCCA CAACCCCAGG ACACGGACAG CGGCGTCGAC 
ACCTTCACTC GCACCATGGA CAACAGGCCA AAATATCAGC ACAACAACAT CAACGCCATT 
CCCAAAGCCG TCCCAGTCAG CCCCAGTGCA CTGGATGATG ATGAAGAAGA TGAGGGACAC 
ACAGTTGTGG CCACGGCGCG GGGCATCTTT AACTGTAATG GCGGGGTGTT GAGCTCTATA 
GAGACGGGCG TCAGCATCAT TATCCCACAG GGAGCCATTC CTGATAGTGT GGAGCAGGAG 
ATCTACTTCA AAGTGTGCCG GGACAACAGC ATCCTGCCTC CTCTGGATAA GGAGAAAGGT 
GAAACTCTGC TGAGTCCGCT GGTGATGTGT GGTCCTCACG GCCTGAAGTT CCTGAAGCCT 
GTGGAGCTGC GCTTGCCACA CTGTGCGTCT ATGACCCCTG ATGGTTGGTC TTTTGCTCTA 
AAATCCTCCG ACTCCTCGTC GGGTGACCCC AAATGCTGGC AAAACAAGTG TCTACCAGGA 
GACCCCAATT ACCTGGTGGG CGCAAACTGT GTGTCTGTGC TGATCGACCA CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp1b ( XM_009298137.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296412.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X11, mRNA.

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CloneID	ODa44627
Clone ID Related Accession (Same CDS sequence)	XM_009298137.3 , XM_009298137.2
Accession Version	XM_009298137.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5817bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tight junction protein ZO-1 isoform X11
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298137.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
TCCCGCAAGA ATGAAGATAT TTCAGACATC CACTCCGTGG CCTCTGATCA TTCCAACCGA 
TCCCACGACC GGAAGAGGAG CAGCCGCTCT CGTTCTCCAG ACCGCCGCTC CGAACCCTCA 
GACCACTCCA GACACTCTCC ACCACAGATC AGCAACGGCA GGGCCACAGG GCCGACGGCT 
CAGAGAACGC GGCCAGTTCA CAGGAGCCGT GATGAAGACA GGATCTCCAA GGCCGGGCCG 
CTTCCTCTAC CTGCTAAGAT GGTGGAGGAG ATGCCGAAAG AGCAGAGCGC AGCCAGAGAA 
ACAGAGAAAC AGCTCCCCCC TCTGCCAGAG CCCAAACCTG TGTACGCTCA GCCGGGCCAG 
CCGGACGTGG ACCTGCCTGT TAGTCCGGCA GATGCTCCTG TGCCCAGCGT CACACATGAT 
GACAGCATCT TACGGCCGAG CATGAAGCTG GTGAAGTTCA AGAAGGGCGA GAGCGTGGGC 
TTGAGGCTCG CTGGGGGGAA CGACGTTGGG ATATTTGTGG CGGGAGTTCT GGAGGACAGT 
CCTGCTGCTA AAGAGGGCCT GGAAGAGGGA GACCAGATTC TCAGGGTAAA TAATGTGGAT 
TTTGCAAATA TCATCCGAGA GGAGGCAGTG CTGTTCCTGC TGGATCTGCC CAAAGGAGAG 
GAGGTCACCA TCCTGGCCCA GAAGAAGAAA GACGTGTACC GCCGCATTGT AGAGTCTGAC 
GTGGGAGACT CGTTCTACAT CAGGACTCAT TTTGAATATG AGAAGGAGTC GCCCTATGGC 
CTGAGCTTCA ATAAGGGGGA GGTCTTCAGA GTCGTCGACA CCCTTTACAA TGGCAAACTG 
GGCTCCTGGC TCGCCATCCG CATCGGCAAA AACCACCAGG AGGTGGAGCG GGGCATCATC 
CCCAACAAGA ACAGGGCTGA GCAGTTATCA AGTGTCCAGT ACACTCTCCC AAAGACTCCA 
GGAGGGGATC GCGCCGACTT CTGGAGATTC AGAGGCCTCC GCAGCTCCAA ACGAAACCTG 
AGGAAGAGCA GAGAAGACCT GTCTGCTCAG CCGGTCCAAA CCAAATTCCC TGCTTATGAA 
AGAGTGGTGC TTAGAGAAGC TGGTTTCTTG AGGCCAGTGG TCATTTTCGG ACCGATCGCG 
GATGTGGCTA GAGAGAAGCT GTCCAGAGAG GAGACTGACC TCTTCGAACT TGCAAAGAGT 
GAACCCAGAG ATGCGGGCAC AGACCAGCGC AGCTCCGGAA TCATCCGTCT CCATACCATT 
AAACAGATCA TTGACCGAGA CAAGCACGCG GTGCTGGACA TCACCCCTAA TGCGGTGGAC 
CGTCTGAACT ACGCTCAGTG GTACCCCATC GTGGTGTTTC TGAATCCAGA CAGCAAACAG 
GGCGTCAAGA ACATGAGGAC CAGGCTCTGT CCCGAGTCCA GGAAGAGCGC CCGAAAGCTC 
TACGAGCGTG CTCTCAAACT GAGGAAGAAC AACCATCACC TTTTCACCAC CACTATTAAC 
TTGAACAACA TGAATGACGG GTGGTACGGT GCTTTGAAAG AGAGCATCCA GCAGCAGCAG 
AACCAGTTGG TCTGGGTCTC AGAGGGCAAG GCCGACGGCG CTCCTGACGA TGACCTTGAC 
ATCCATGACG ACCGCCTCTC CTACCTCTCG GCTCCGGGCA GCGAGTACAG CATGTACAGC 
ACCGACAGCC GCCACACGTC CGACTATGAG GACACCGACA CGGAGGGTGG TGCCTACACC 
GACCAGGAGC TGGACGAGAC CATGAACGAT GAGGTGGGCC TGCCTAGCGA GCCTGCCATC 
ACACGCTCAT CTGAGCCTGT GCGAGAGGAC CCGCCAGTCA TTCAGGACGC AGCTGGATAC 
CCCAGCTACC AGCATGCGGT ACCGCAACCT GAAGCGGTGA GCCGCATTGA CCCGGCCGGG 
TTTAAGATGG CAGCGCCGCA GCAGCAAGCT GAGGCTGCTC TGGCCTCGCC GCCCCCTCCC 
TCTGCAGCGG TCCAGCCCAC CGCAACACTA GCGGGTACGA ACCCCGAGGA GCCGCCTGGC 
ACCCCTCAGG CCGACTCCCT TAACAGCCCC GTCCCTGTTC CCGACCCCCA GCCCGAACCT 
GAGCTCGCTC AGCCCCCAAC ACACGACCCC CACCAGTCGC TTCCTCCTGG CCCAGATCCA 
AAGATGTATA AAAAGGATCT GTACAGCATG GACGAGCCGG TGCGAGTGAC CCACGGCGTG 
AAGCCTCCCC CTCCCCAGCA GCAGCAGCAG CCTTTAGGCC CCCCAACCTC GCTCTCCTAC 
AGCCACCAGC CTGTCTACCA GGACCAACAG CCATACCGCC AGTACGACCA CCCGCCTTAT 
GGCTATGACG GTGGCGGCTA CGCACAACCA AAGCCTCACA ACTACGACCC GCACCTGCAC 
TACGACAACC GTGTGCCTCA CTATAACGAG CAGTGGCCCA CGTACGACCA GCAGCAGCAG 
CAGCAGACTT CCCCCCAGCC GCCACCTGCG TCCGGCTTCC CTCAGGGCCA CCAGCCTCCG 
CCTCCACCAC CGATGGGATA TGAGCCACGC TCCCCATACG AGGACGGACC TAACAGGGAT 
TTCAGCCCTC CTCAGTCCCA GTATGACGGT GTGTCCCCAA TGGGCTACGA TCACCGGCCG 
CGGCACCCCA AACCTGCACC GGTCCGATAT GAAGAGCCTC CACCTCCACC TCCTCCGGTG 
TCCTACGATG CACGGTCACC TTTCGAACCC GAGCCGCATG CTTTCCCAGG CAACACACAT 
CGCTCGCCAG ACCCACCGAA ACAGTATTAT GGAGACGCCG CTATGAGGCC CTTGTACAAC 
CCTGGACCTC CGAACAGAGT GTACAAACAT GAGCCGGTGA ACTCTGAGCC TCCTGTACCG 
CCTCCCAAAC CTGAATCTGT CCTGTCTCCA GGAGAACCGC CCCATTCTGC AGCTTCCAAA 
CCATTGCCGC CTCCTCCCAG AGATGATGAC GATGATGATG ACGATGATGA TGACCCTGCC 
ATGAAGCCCC AGTCTGTGCT CAACAGGGTC AAAATGTTCG AAAACAAACG CTCTGTGTCG 
GTAGACCGAG CCAAGGACGC ACCTGACGTC ACTGGAATTA GGCCCACAGA TCTCCCCAAA 
CCTGCGAGCA CCCCTGGACC TGTGCTCAAA GCCAACTCTC TCAGCAACCT GGAGCAGGAC 
AAACCCTCCT ACAGAGCTCC AGAGCCGCAG AAACCTCAGA CGCGTGTTGG GGACGATGTG 
GTTCGCTCCA ACCACTACGA CCCTGATGAT GATGAAGAGT ACTACAGAAA ACAGCTCTCA 
TTTTTCGACC GCCGCAGTTT CGACAGTAAG GTCATGAATC CACCCAGCGC TGGAGTAAAC 
CGCTTCCATG AGCCGCCCAA ACCACCCCAG ACACAGATCG CTTATCCTTT CGCTAGGACG 
GAGTCTGTGG AGAAGGTGAG TCCTGTGGAC AAGAGATACG AGCCTCTGCC GCCAGTCAAC 
CCTTCACCTG CTCCATACAG CCAGAACACA CCTGTCGCTC CGCCCACATC CCTGCCCAAA 
CTCACCAACA TCGAGGTGAA CTCTCTGCCA GATCCTCACA GCTCTCCCAA AGCCAAACCG 
GACCTGTCTG CTCTGAGAGC TCCGACCCGG GACGAGCCCA TCCAGAGCAG CTACTTGCCC 
CCCAAGTCCA CCATCAATGG CATGGATGCT CCTCCCAAAA CACTCGGCGT GCCGACCAGC 
TACAACCGCT ACGTCCCCAA GCCTTACACC AGCTCGGCGC GACCCTTCGA GCGCAAGTTC 
GAGAGTCCGA AATTCAACCA CAACCTGCTT CCCAACGACA CGCAGTCCAA ACCCAGTGTC 
AACAACAACC TGAAGCCTCA GATCTCCCCA CAACCCCAGG ACACGGACAG CGGCGTCGAC 
ACCTTCACTC GCACCATGGA CAACAGGCCA AAATATCAGC ACAACAACAT CAACGCCATT 
CCCAAAGCCG TCCCAGTCAG CCCCAGTGCA CTGGATGATG ATGAAGAAGA TGAGGGACAC 
ACAGTTGTGG CCACGGCGCG GGGCATCTTT AACTGTAATG GCGGGGTGTT GAGCTCTATA 
GAGACGGGCG TCAGCATCAT TATCCCACAG GGAGCCATTC CTGATAGTGT GGAGCAGGAG 
ATCTACTTCA AAGTGTGCCG GGACAACAGC ATCCTGCCTC CTCTGGATAA GGAGAAAGGT 
GAAACTCTGC TGAGTCCGCT GGTGATGTGT GGTCCTCACG GCCTGAAGTT CCTGAAGCCT 
GTGGAGCTGC GCTTGCCACA CTGTGCGTCT ATGACCCCTG ATGGTTGGTC TTTTGCTCTA 
AAATCCTCCG ACTCCTCGTC GGGTGACCCC AAATGCTGGC AAAACAAGTG TCTACCAGGA 
GACCCCAATT ACCTGGTGGG CGCAAACTGT GTGTCTGTGC TGATCGACCA CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296412.1
CDS	115..5931
Translation	MLLSVFGGPVEEVLAGLLKLKMFGSGSKKWFIKKNKRQRTKVQRKPASGRRNKRVSRVLKRHKEVIVKQLDVSKVLPTLVYERVFTLGEYKEILGQDSSGKRAELFLDLLSQKEPAAFSAFCGVLEEVCPHLLTCFLLDGTDGSAGENKKQGQTKPAHSQQNALSKGQDTVCFPPMYTDPLFDTRVFPKHDKDKYGPAVYYPKFNAYIPNPDKVSVLDESEGSGHGKPSLRRIKGRIHRSKSLDSIDLLDSNSAAMDETVIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGLLQENDRVVMVNAVSMDNVDHAYAVQQLRKSGKNAKITIRRKRKVQIPSGRPGERETMSEHDEDDDSYEDEIYESRSARSGPSAYSGGGGGGGGGGASGRRSGRGDRLSGRRERDRERSGSRERSLSPRSDRHSVSSNLPPRPAKVTLVKSRKNEDISDIHSVASDHSNRSHDRKRSSRSRSPDRRSEPSDHSRHSPPQISNGRATGPTAQRTRPVHRSRDEDRISKAGPLPLPAKMVEEMPKEQSAARETEKQLPPLPEPKPVYAQPGQPDVDLPVSPADAPVPSVTHDDSILRPSMKLVKFKKGESVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFANIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHQEVERGIIPNKNRAEQLSSVQYTLPKTPGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVVIFGPIADVAREKLSREETDLFELAKSEPRDAGTDQRSSGIIRLHTIKQIIDRDKHAVLDITPNAVDRLNYAQWYPIVVFLNPDSKQGVKNMRTRLCPESRKSARKLYERALKLRKNNHHLFTTTINLNNMNDGWYGALKESIQQQQNQLVWVSEGKADGAPDDDLDIHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETMNDEVGLPSEPAITRSSEPVREDPPVIQDAAGYPSYQHAVPQPEAVSRIDPAGFKMAAPQQQAEAALASPPPPSAAVQPTATLAGTNPEEPPGTPQADSLNSPVPVPDPQPEPELAQPPTHDPHQSLPPGPDPKMYKKDLYSMDEPVRVTHGVKPPPPQQQQQPLGPPTSLSYSHQPVYQDQQPYRQYDHPPYGYDGGGYAQPKPHNYDPHLHYDNRVPHYNEQWPTYDQQQQQQTSPQPPPASGFPQGHQPPPPPPMGYEPRSPYEDGPNRDFSPPQSQYDGVSPMGYDHRPRHPKPAPVRYEEPPPPPPPVSYDARSPFEPEPHAFPGNTHRSPDPPKQYYGDAAMRPLYNPGPPNRVYKHEPVNSEPPVPPPKPESVLSPGEPPHSAASKPLPPPPRDDDDDDDDDDDPAMKPQSVLNRVKMFENKRSVSVDRAKDAPDVTGIRPTDLPKPASTPGPVLKANSLSNLEQDKPSYRAPEPQKPQTRVGDDVVRSNHYDPDDDEEYYRKQLSFFDRRSFDSKVMNPPSAGVNRFHEPPKPPQTQIAYPFARTESVEKVSPVDKRYEPLPPVNPSPAPYSQNTPVAPPTSLPKLTNIEVNSLPDPHSSPKAKPDLSALRAPTRDEPIQSSYLPPKSTINGMDAPPKTLGVPTSYNRYVPKPYTSSARPFERKFESPKFNHNLLPNDTQSKPSVNNNLKPQISPQPQDTDSGVDTFTRTMDNRPKYQHNNINAIPKAVPVSPSALDDDEEDEGHTVVATARGIFNCNGGVLSSIETGVSIIIPQGAIPDSVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKCWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_009298137.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tight junction protein 1b (tjp1b), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298137.2

ORF Insert Sequence:

ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
TCCCGCAAGA ATGAAGATAT TTCAGACATC CACTCCGTGG CCTCTGATCA TTCCAACCGA 
TCCCACGACC GGAAGAGGAG CAGCCGCTCT CGTTCTCCAG ACCGCCGCTC CGAACCCTCA 
GACCACTCCA GACACTCTCC ACCACAGATC AGCAACGGCA GGGCCACAGG GCCGACGGCT 
CAGAGAACGC GGCCAGTTCA CAGGAGCCGT GATGAAGACA GGATCTCCAA GGCCGGGCCG 
CTTCCTCTAC CTGCTAAGAT GGTGGAGGAG ATGCCGAAAG AGCAGAGCGC AGCCAGAGAA 
ACAGAGAAAC AGCTCCCCCC TCTGCCAGAG CCCAAACCTG TGTACGCTCA GCCGGGCCAG 
CCGGACGTGG ACCTGCCTGT TAGTCCGGCA GATGCTCCTG TGCCCAGCGT CACACATGAT 
GACAGCATCT TACGGCCGAG CATGAAGCTG GTGAAGTTCA AGAAGGGCGA GAGCGTGGGC 
TTGAGGCTCG CTGGGGGGAA CGACGTTGGG ATATTTGTGG CGGGAGTTCT GGAGGACAGT 
CCTGCTGCTA AAGAGGGCCT GGAAGAGGGA GACCAGATTC TCAGGGTAAA TAATGTGGAT 
TTTGCAAATA TCATCCGAGA GGAGGCAGTG CTGTTCCTGC TGGATCTGCC CAAAGGAGAG 
GAGGTCACCA TCCTGGCCCA GAAGAAGAAA GACGTGTACC GCCGCATTGT AGAGTCTGAC 
GTGGGAGACT CGTTCTACAT CAGGACTCAT TTTGAATATG AGAAGGAGTC GCCCTATGGC 
CTGAGCTTCA ATAAGGGGGA GGTCTTCAGA GTCGTCGACA CCCTTTACAA TGGCAAACTG 
GGCTCCTGGC TCGCCATCCG CATCGGCAAA AACCACCAGG AGGTGGAGCG GGGCATCATC 
CCCAACAAGA ACAGGGCTGA GCAGTTATCA AGTGTCCAGT ACACTCTCCC AAAGACTCCA 
GGAGGGGATC GCGCCGACTT CTGGAGATTC AGAGGCCTCC GCAGCTCCAA ACGAAACCTG 
AGGAAGAGCA GAGAAGACCT GTCTGCTCAG CCGGTCCAAA CCAAATTCCC TGCTTATGAA 
AGAGTGGTGC TTAGAGAAGC TGGTTTCTTG AGGCCAGTGG TCATTTTCGG ACCGATCGCG 
GATGTGGCTA GAGAGAAGCT GTCCAGAGAG GAGACTGACC TCTTCGAACT TGCAAAGAGT 
GAACCCAGAG ATGCGGGCAC AGACCAGCGC AGCTCCGGAA TCATCCGTCT CCATACCATT 
AAACAGATCA TTGACCGAGA CAAGCACGCG GTGCTGGACA TCACCCCTAA TGCGGTGGAC 
CGTCTGAACT ACGCTCAGTG GTACCCCATC GTGGTGTTTC TGAATCCAGA CAGCAAACAG 
GGCGTCAAGA ACATGAGGAC CAGGCTCTGT CCCGAGTCCA GGAAGAGCGC CCGAAAGCTC 
TACGAGCGTG CTCTCAAACT GAGGAAGAAC AACCATCACC TTTTCACCAC CACTATTAAC 
TTGAACAACA TGAATGACGG GTGGTACGGT GCTTTGAAAG AGAGCATCCA GCAGCAGCAG 
AACCAGTTGG TCTGGGTCTC AGAGGGCAAG GCCGACGGCG CTCCTGACGA TGACCTTGAC 
ATCCATGACG ACCGCCTCTC CTACCTCTCG GCTCCGGGCA GCGAGTACAG CATGTACAGC 
ACCGACAGCC GCCACACGTC CGACTATGAG GACACCGACA CGGAGGGTGG TGCCTACACC 
GACCAGGAGC TGGACGAGAC CATGAACGAT GAGGTGGGCC TGCCTAGCGA GCCTGCCATC 
ACACGCTCAT CTGAGCCTGT GCGAGAGGAC CCGCCAGTCA TTCAGGACGC AGCTGGATAC 
CCCAGCTACC AGCATGCGGT ACCGCAACCT GAAGCGGTGA GCCGCATTGA CCCGGCCGGG 
TTTAAGATGG CAGCGCCGCA GCAGCAAGCT GAGGCTGCTC TGGCCTCGCC GCCCCCTCCC 
TCTGCAGCGG TCCAGCCCAC CGCAACACTA GCGGGTACGA ACCCCGAGGA GCCGCCTGGC 
ACCCCTCAGG CCGACTCCCT TAACAGCCCC GTCCCTGTTC CCGACCCCCA GCCCGAACCT 
GAGCTCGCTC AGCCCCCAAC ACACGACCCC CACCAGTCGC TTCCTCCTGG CCCAGATCCA 
AAGATGTATA AAAAGGATCT GTACAGCATG GACGAGCCGG TGCGAGTGAC CCACGGCGTG 
AAGCCTCCCC CTCCCCAGCA GCAGCAGCAG CCTTTAGGCC CCCCAACCTC GCTCTCCTAC 
AGCCACCAGC CTGTCTACCA GGACCAACAG CCATACCGCC AGTACGACCA CCCGCCTTAT 
GGCTATGACG GTGGCGGCTA CGCACAACCA AAGCCTCACA ACTACGACCC GCACCTGCAC 
TACGACAACC GTGTGCCTCA CTATAACGAG CAGTGGCCCA CGTACGACCA GCAGCAGCAG 
CAGCAGACTT CCCCCCAGCC GCCACCTGCG TCCGGCTTCC CTCAGGGCCA CCAGCCTCCG 
CCTCCACCAC CGATGGGATA TGAGCCACGC TCCCCATACG AGGACGGACC TAACAGGGAT 
TTCAGCCCTC CTCAGTCCCA GTATGACGGT GTGTCCCCAA TGGGCTACGA TCACCGGCCG 
CGGCACCCCA AACCTGCACC GGTCCGATAT GAAGAGCCTC CACCTCCACC TCCTCCGGTG 
TCCTACGATG CACGGTCACC TTTCGAACCC GAGCCGCATG CTTTCCCAGG CAACACACAT 
CGCTCGCCAG ACCCACCGAA ACAGTATTAT GGAGACGCCG CTATGAGGCC CTTGTACAAC 
CCTGGACCTC CGAACAGAGT GTACAAACAT GAGCCGGTGA ACTCTGAGCC TCCTGTACCG 
CCTCCCAAAC CTGAATCTGT CCTGTCTCCA GGAGAACCGC CCCATTCTGC AGCTTCCAAA 
CCATTGCCGC CTCCTCCCAG AGATGATGAC GATGATGATG ACGATGATGA TGACCCTGCC 
ATGAAGCCCC AGTCTGTGCT CAACAGGGTC AAAATGTTCG AAAACAAACG CTCTGTGTCG 
GTAGACCGAG CCAAGGACGC ACCTGACGTC ACTGGAATTA GGCCCACAGA TCTCCCCAAA 
CCTGCGAGCA CCCCTGGACC TGTGCTCAAA GCCAACTCTC TCAGCAACCT GGAGCAGGAC 
AAACCCTCCT ACAGAGCTCC AGAGCCGCAG AAACCTCAGA CGCGTGTTGG GGACGATGTG 
GTTCGCTCCA ACCACTACGA CCCTGATGAT GATGAAGAGT ACTACAGAAA ACAGCTCTCA 
TTTTTCGACC GCCGCAGTTT CGACAGTAAG GTCATGAATC CACCCAGCGC TGGAGTAAAC 
CGCTTCCATG AGCCGCCCAA ACCACCCCAG ACACAGATCG CTTATCCTTT CGCTAGGACG 
GAGTCTGTGG AGAAGGTGAG TCCTGTGGAC AAGAGATACG AGCCTCTGCC GCCAGTCAAC 
CCTTCACCTG CTCCATACAG CCAGAACACA CCTGTCGCTC CGCCCACATC CCTGCCCAAA 
CTCACCAACA TCGAGGTGAA CTCTCTGCCA GATCCTCACA GCTCTCCCAA AGCCAAACCG 
GACCTGTCTG CTCTGAGAGC TCCGACCCGG GACGAGCCCA TCCAGAGCAG CTACTTGCCC 
CCCAAGTCCA CCATCAATGG CATGGATGCT CCTCCCAAAA CACTCGGCGT GCCGACCAGC 
TACAACCGCT ACGTCCCCAA GCCTTACACC AGCTCGGCGC GACCCTTCGA GCGCAAGTTC 
GAGAGTCCGA AATTCAACCA CAACCTGCTT CCCAACGACA CGCAGTCCAA ACCCAGTGTC 
AACAACAACC TGAAGCCTCA GATCTCCCCA CAACCCCAGG ACACGGACAG CGGCGTCGAC 
ACCTTCACTC GCACCATGGA CAACAGGCCA AAATATCAGC ACAACAACAT CAACGCCATT 
CCCAAAGCCG TCCCAGTCAG CCCCAGTGCA CTGGATGATG ATGAAGAAGA TGAGGGACAC 
ACAGTTGTGG CCACGGCGCG GGGCATCTTT AACTGTAATG GCGGGGTGTT GAGCTCTATA 
GAGACGGGCG TCAGCATCAT TATCCCACAG GGAGCCATTC CTGATAGTGT GGAGCAGGAG 
ATCTACTTCA AAGTGTGCCG GGACAACAGC ATCCTGCCTC CTCTGGATAA GGAGAAAGGT 
GAAACTCTGC TGAGTCCGCT GGTGATGTGT GGTCCTCACG GCCTGAAGTT CCTGAAGCCT 
GTGGAGCTGC GCTTGCCACA CTGTGCGTCT ATGACCCCTG ATGGTTGGTC TTTTGCTCTA 
AAATCCTCCG ACTCCTCGTC GGGTGACCCC AAATGCTGGC AAAACAAGTG TCTACCAGGA 
GACCCCAATT ACCTGGTGGG CGCAAACTGT GTGTCTGTGC TGATCGACCA CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp1b ( XM_021470734.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326409.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X17, mRNA.

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CloneID	ODa44628
Clone ID Related Accession (Same CDS sequence)	XM_021470734.1
Accession Version	XM_021470734.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5676bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tight junction protein ZO-1 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAATT ACCAGAAATA CATTACTGTT ATGCAGCTGG CTCTAGGGGT GACTGCCTAC 
AACAGAGACC GTAGCCTGCC TCCCAGAAAA CATATGTGGG TCTTCCCCAA ACACGACAAA 
GACAAATACG GCCCTGCAGT TTATTATCCG AAGTTCAACG CCTACATCCC CAACCCAGAC 
AAAGTCTCTG TGCTGGATGA ATCTGAGGGA TCGGGCCACG GCAAACCATC CCTGCGCAGA 
ATCAAGGGCC GAATCCACCG CAGCAAGAGC CTGGACAGCA TCGACCTGCT GGACTCCAAC 
AGTGCAGCAA TGGATGAGAC GGTAATATGG GAACAGCACA CTGTAACTCT ACACAGGGCT 
CCAGGGTTTG GCTTCGGGAT CGCCATCTCC GGCGGACGGG ACAACCCTCA CTTCCAGAGC 
GGGGAGACGT CCATCGTCAT ATCAGATGTG CTGAAGGGAG GCCCCGCCGA GGGCCTGCTG 
CAGGAGAATG ACAGAGTGGT GATGGTCAAC GCCGTCTCCA TGGACAATGT GGATCACGCT 
TACGCAGTGC AGCAGCTCCG CAAAAGTGGC AAGAATGCAA AAATAACCAT CAGGAGAAAG 
CGTAAGGTGC AGATCCCGTC AGGCCGGCCG GGTGAACGCG AGACTATGTC AGAGCATGAC 
GAGGACGACG ACAGCTACGA GGATGAGATC TACGAGTCCC GGAGCGCGCG AAGCGGACCC 
AGCGCCTACA GTGGAGGTGG AGGCGGCGGC GGAGGTGGAG GAGCCTCGGG ACGGAGGAGC 
GGCAGAGGCG ACAGGCTGAG TGGGAGGAGG GAGCGGGACA GAGAGCGCAG CGGCTCCAGA 
GAGAGGAGTC TGTCCCCGCG CTCGGACCGC CACTCTGTGT CCTCCAACCT CCCCCCACGG 
CCAGCCAAAG TCACACTCGT CAAATCCCGC AAGAATGAAG CATGTATGGC GTCAGGAAGT 
TCTCAGAAGG TGACATCAGC AGAATACGGG CTTCGCCTGG CCAGCCACAT CTTCGTGAAG 
GACATTTCCC CCGAGAGCCT GGCAGCCCGA GACGGAAACA TCCAAGAGGG AGACGTGGTG 
CTTAAGATTA ATGGCACGGT AACAGAGAAT TTGTCCCTGA TCGATGCTAA GAAGCTGATC 
GAGAGGTCAA AGGGAAAGCT GAAGATGGTG GTGCAGAGAG ACGAGCGGGC GACGCTGCTG 
AACATCCCCG ATCTGGACGA CAGCATACCT TCTGCTAATG CCTCGGACAG AGATGATATT 
TCAGACATCC ACTCCGTGGC CTCTGATCAT TCCAACCGAT CCCACGACCG GAAGAGGAGC 
AGCCGCTCTC GTTCTCCAGA CCGCCGCTCC GAACCCTCAG ACCACTCCAG ACACTCTCCA 
CCACAGATCA GCAACGGCAG GGCCACAGGG CCGACGGCTC AGAGAACGCG GCCAGTTCAC 
AGGAGCCGTG ATGAAGACAG GATCTCCAAG GCCGGGCCGC TTCCTCTACC TGCTAAGATG 
GTGGAGGAGA TGCCGAAAGA GCAGAGCGCA GCCAGAGAAA CAGAGAAACA GCTCCCCCCT 
CTGCCAGAGC CCAAACCTGT GTACGCTCAG CCGGGCCAGC CGGACGTGGA CCTGCCTGTT 
AGTCCGGCAG ATGCTCCTGT GCCCAGCGTC ACACATGATG ACAGCATCTT ACGGCCGAGC 
ATGAAGCTGG TGAAGTTCAA GAAGGGCGAG AGCGTGGGCT TGAGGCTCGC TGGGGGGAAC 
GACGTTGGGA TATTTGTGGC GGGAGTTCTG GAGGACAGTC CTGCTGCTAA AGAGGGCCTG 
GAAGAGGGAG ACCAGATTCT CAGGGTAAAT AATGTGGATT TTGCAAATAT CATCCGAGAG 
GAGGCAGTGC TGTTCCTGCT GGATCTGCCC AAAGGAGAGG AGGTCACCAT CCTGGCCCAG 
AAGAAGAAAG ACGTGTACCG CCGCATTGTA GAGTCTGACG TGGGAGACTC GTTCTACATC 
AGGACTCATT TTGAATATGA GAAGGAGTCG CCCTATGGCC TGAGCTTCAA TAAGGGGGAG 
GTCTTCAGAG TCGTCGACAC CCTTTACAAT GGCAAACTGG GCTCCTGGCT CGCCATCCGC 
ATCGGCAAAA ACCACCAGGA GGTGGAGCGG GGCATCATCC CCAACAAGAA CAGGGCTGAG 
CAGTTATCAA GTGTCCAGTA CACTCTCCCA AAGACTCCAG GAGGGGATCG CGCCGACTTC 
TGGAGATTCA GAGGCCTCCG CAGCTCCAAA CGAAACCTGA GGAAGAGCAG AGAAGACCTG 
TCTGCTCAGC CGGTCCAAAC CAAATTCCCT GCTTATGAAA GAGTGGTGCT TAGAGAAGCT 
GGTTTCTTGA GGCCAGTGGT CATTTTCGGA CCGATCGCGG ATGTGGCTAG AGAGAAGCTG 
TCCAGAGAGG AGACTGACCT CTTCGAACTT GCAAAGAGTG AACCCAGAGA TGCGGGCACA 
GACCAGCGCA GCTCCGGAAT CATCCGTCTC CATACCATTA AACAGATCAT TGACCGAGAC 
AAGCACGCGG TGCTGGACAT CACCCCTAAT GCGGTGGACC GTCTGAACTA CGCTCAGTGG 
TACCCCATCG TGGTGTTTCT GAATCCAGAC AGCAAACAGG GCGTCAAGAA CATGAGGACC 
AGGCTCTGTC CCGAGTCCAG GAAGAGCGCC CGAAAGCTCT ACGAGCGTGC TCTCAAACTG 
AGGAAGAACA ACCATCACCT TTTCACCACC ACTATTAACT TGAACAACAT GAATGACGGG 
TGGTACGGTG CTTTGAAAGA GAGCATCCAG CAGCAGCAGA ACCAGTTGGT CTGGGTCTCA 
GAGGGCAAGG CCGACGGCGC TCCTGACGAT GACCTTGACA TCCATGACGA CCGCCTCTCC 
TACCTCTCGG CTCCGGGCAG CGAGTACAGC ATGTACAGCA CCGACAGCCG CCACACGTCC 
GACTATGAGG ACACCGACAC GGAGGGTGGT GCCTACACCG ACCAGGAGCT GGACGAGACC 
ATGAACGATG AGGTGGGCCT GCCTAGCGAG CCTGCCATCA CACGCTCATC TGAGCCTGTG 
CGAGAGGACC CGCCAGTCAT TCAGGACGCA GCTGGATACC CCAGCTACCA GCATGCGGTA 
CCGCAACCTG AAGCGGTGAG CCGCATTGAC CCGGCCGGGT TTAAGATGGC AGCGCCGCAG 
CAGCAAGCTG AGGCTGCTCT GGCCTCGCCG CCCCCTCCCT CTGCAGCGGT CCAGCCCACC 
GCAACACTAG CGGGTACGAA CCCCGAGGAG CCGCCTGGCA CCCCTCAGGC CGACTCCCTT 
AACAGCCCCG TCCCTGTTCC CGACCCCCAG CCCGAACCTG AGCTCGCTCA GCCCCCAACA 
CACGACCCCC ACCAGTCGCT TCCTCCTGGC CCAGATCCAA AGATGTATAA AAAGGATCTG 
TACAGCATGG ACGAGCCGGT GCGAGTGACC CACGGCGTGA AGCCTCCCCC TCCCCAGCAG 
CAGCAGCAGC CTTTAGGCCC CCCAACCTCG CTCTCCTACA GCCACCAGCC TGTCTACCAG 
GACCAACAGC CATACCGCCA GTACGACCAC CCGCCTTATG GCTATGACGG TGGCGGCTAC 
GCACAACCAA AGCCTCACAA CTACGACCCG CACCTGCACT ACGACAACCG TGTGCCTCAC 
TATAACGAGC AGTGGCCCAC GTACGACCAG CAGCAGCAGC AGCAGACTTC CCCCCAGCCG 
CCACCTGCGT CCGGCTTCCC TCAGGGCCAC CAGCCTCCGC CTCCACCACC GATGGGATAT 
GAGCCACGCT CCCCATACGA GGACGGACCT AACAGGGATT TCAGCCCTCC TCAGTCCCAG 
TATGACGGTG TGTCCCCAAT GGGCTACGAT CACCGGCCGC GGCACCCCAA ACCTGCACCG 
GTCCGATATG AAGAGCCTCC ACCTCCACCT CCTCCGGTGT CCTACGATGC ACGGTCACCT 
TTCGAACCCG AGCCGCATGC TTTCCCAGGC AACACACATC GCTCGCCAGA CCCACCGAAA 
CAGTATTATG GAGACGCCGC TATGAGGCCC TTGTACAACC CTGGACCTCC GAACAGAGTG 
TACAAACATG AGCCGGTGAA CTCTGAGCCT CCTGTACCGC CTCCCAAACC TGAATCTGTC 
CTGTCTCCAG GAGAACCGCC CCATTCTGCA GCTTCCAAAC CATTGCCGCC TCCTCCCAGA 
GATGATGACG ATGATGATGA CGATGATGAT GACCCTGCCA TGAAGCCCCA GTCTGTGCTC 
AACAGGGTCA AAATGTTCGA AAACAAACGC TCTGTGTCGG TAGACCGAGC CAAGGACGCA 
CCTGACGTCA CTGGAATTAG GCCCACAGAT CTCCCCAAAC CTGCGAGCAC CCCTGGACCT 
GTGCTCAAAG CCAACTCTCT CAGCAACCTG GAGCAGGACA AACCCTCCTA CAGAGCTCCA 
GAGCCGCAGA AACCTCAGAC GCGTGTTGGG GACGATGTGG TTCGCTCCAA CCACTACGAC 
CCTGATGATG ATGAAGAGTA CTACAGAAAA CAGCTCTCAT TTTTCGACCG CCGCAGTTTC 
GACAGTAAGG TCATGAATCC ACCCAGCGCT GGAGTAAACC GCTTCCATGA GCCGCCCAAA 
CCACCCCAGA CACAGATCGC TTATCCTTTC GCTAGGACGG AGTCTGTGGA GAAGGTGAGT 
CCTGTGGACA AGAGATACGA GCCTCTGCCG CCAGTCAACC CTTCACCTGC TCCATACAGC 
CAGAACACAC CTGTCGCTCC GCCCACATCC CTGCCCAAAC TCACCAACAT CGAGGTGAAC 
TCTCTGCCAG ATCCTCACAG CTCTCCCAAA GCCAAACCGG ACCTGTCTGC TCTGAGAGCT 
CCGACCCGGG ACGAGCCCAT CCAGAGCAGC TACTTGCCCC CCAAGTCCAC CATCAATGGC 
ATGGATGCTC CTCCCAAAAC ACTCGGCGTG CCGACCAGCT ACAACCGCTA CGTCCCCAAG 
CCTTACACCA GCTCGGCGCG ACCCTTCGAG CGCAAGTTCG AGAGTCCGAA ATTCAACCAC 
AACCTGCTTC CCAACGACAC GCAGTCCAAA CCCAGTGTCA ACAACAACCT GAAGCCTCAG 
ATCTCCCCAC AACCCCAGGA CACGGACAGC GGCGTCGACA CCTTCACTCG CACCATGGAC 
AACAGGCCAA AATATCAGCA CAACAACATC AACGCCATTC CCAAAGCCGT CCCAGTCAGC 
CCCAGTGCAC TGGATGATGA TGAAGAAGAT GAGGGACACA CAGTTGTGGC CACGGCGCGG 
GGCATCTTTA ACTGTAATGG CGGGGTGTTG AGCTCTATAG AGACGGGCGT CAGCATCATT 
ATCCCACAGG GAGCCATTCC TGATAGTGTG GAGCAGGAGA TCTACTTCAA AGTGTGCCGG 
GACAACAGCA TCCTGCCTCC TCTGGATAAG GAGAAAGGTG AAACTCTGCT GAGTCCGCTG 
GTGATGTGTG GTCCTCACGG CCTGAAGTTC CTGAAGCCTG TGGAGCTGCG CTTGCCACAC 
TGTGCGTCTA TGACCCCTGA TGGTTGGTCT TTTGCTCTAA AATCCTCCGA CTCCTCGTCG 
GGTGACCCCA AATGCTGGCA AAACAAGTGT CTACCAGGAG ACCCCAATTA CCTGGTGGGC 
GCAAACTGTG TGTCTGTGCT GATCGACCAC TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326409.1
CDS	427..6102
Translation	MVNYQKYITVMQLALGVTAYNRDRSLPPRKHMWVFPKHDKDKYGPAVYYPKFNAYIPNPDKVSVLDESEGSGHGKPSLRRIKGRIHRSKSLDSIDLLDSNSAAMDETVIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGLLQENDRVVMVNAVSMDNVDHAYAVQQLRKSGKNAKITIRRKRKVQIPSGRPGERETMSEHDEDDDSYEDEIYESRSARSGPSAYSGGGGGGGGGGASGRRSGRGDRLSGRRERDRERSGSRERSLSPRSDRHSVSSNLPPRPAKVTLVKSRKNEACMASGSSQKVTSAEYGLRLASHIFVKDISPESLAARDGNIQEGDVVLKINGTVTENLSLIDAKKLIERSKGKLKMVVQRDERATLLNIPDLDDSIPSANASDRDDISDIHSVASDHSNRSHDRKRSSRSRSPDRRSEPSDHSRHSPPQISNGRATGPTAQRTRPVHRSRDEDRISKAGPLPLPAKMVEEMPKEQSAARETEKQLPPLPEPKPVYAQPGQPDVDLPVSPADAPVPSVTHDDSILRPSMKLVKFKKGESVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFANIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHQEVERGIIPNKNRAEQLSSVQYTLPKTPGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVVIFGPIADVAREKLSREETDLFELAKSEPRDAGTDQRSSGIIRLHTIKQIIDRDKHAVLDITPNAVDRLNYAQWYPIVVFLNPDSKQGVKNMRTRLCPESRKSARKLYERALKLRKNNHHLFTTTINLNNMNDGWYGALKESIQQQQNQLVWVSEGKADGAPDDDLDIHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETMNDEVGLPSEPAITRSSEPVREDPPVIQDAAGYPSYQHAVPQPEAVSRIDPAGFKMAAPQQQAEAALASPPPPSAAVQPTATLAGTNPEEPPGTPQADSLNSPVPVPDPQPEPELAQPPTHDPHQSLPPGPDPKMYKKDLYSMDEPVRVTHGVKPPPPQQQQQPLGPPTSLSYSHQPVYQDQQPYRQYDHPPYGYDGGGYAQPKPHNYDPHLHYDNRVPHYNEQWPTYDQQQQQQTSPQPPPASGFPQGHQPPPPPPMGYEPRSPYEDGPNRDFSPPQSQYDGVSPMGYDHRPRHPKPAPVRYEEPPPPPPPVSYDARSPFEPEPHAFPGNTHRSPDPPKQYYGDAAMRPLYNPGPPNRVYKHEPVNSEPPVPPPKPESVLSPGEPPHSAASKPLPPPPRDDDDDDDDDDDPAMKPQSVLNRVKMFENKRSVSVDRAKDAPDVTGIRPTDLPKPASTPGPVLKANSLSNLEQDKPSYRAPEPQKPQTRVGDDVVRSNHYDPDDDEEYYRKQLSFFDRRSFDSKVMNPPSAGVNRFHEPPKPPQTQIAYPFARTESVEKVSPVDKRYEPLPPVNPSPAPYSQNTPVAPPTSLPKLTNIEVNSLPDPHSSPKAKPDLSALRAPTRDEPIQSSYLPPKSTINGMDAPPKTLGVPTSYNRYVPKPYTSSARPFERKFESPKFNHNLLPNDTQSKPSVNNNLKPQISPQPQDTDSGVDTFTRTMDNRPKYQHNNINAIPKAVPVSPSALDDDEEDEGHTVVATARGIFNCNGGVLSSIETGVSIIIPQGAIPDSVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKCWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_021470734.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tight junction protein 1b (tjp1b), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470734.1

ORF Insert Sequence:

ATGGTGAATT ACCAGAAATA CATTACTGTT ATGCAGCTGG CTCTAGGGGT GACTGCCTAC 
AACAGAGACC GTAGCCTGCC TCCCAGAAAA CATATGTGGG TCTTCCCCAA ACACGACAAA 
GACAAATACG GCCCTGCAGT TTATTATCCG AAGTTCAACG CCTACATCCC CAACCCAGAC 
AAAGTCTCTG TGCTGGATGA ATCTGAGGGA TCGGGCCACG GCAAACCATC CCTGCGCAGA 
ATCAAGGGCC GAATCCACCG CAGCAAGAGC CTGGACAGCA TCGACCTGCT GGACTCCAAC 
AGTGCAGCAA TGGATGAGAC GGTAATATGG GAACAGCACA CTGTAACTCT ACACAGGGCT 
CCAGGGTTTG GCTTCGGGAT CGCCATCTCC GGCGGACGGG ACAACCCTCA CTTCCAGAGC 
GGGGAGACGT CCATCGTCAT ATCAGATGTG CTGAAGGGAG GCCCCGCCGA GGGCCTGCTG 
CAGGAGAATG ACAGAGTGGT GATGGTCAAC GCCGTCTCCA TGGACAATGT GGATCACGCT 
TACGCAGTGC AGCAGCTCCG CAAAAGTGGC AAGAATGCAA AAATAACCAT CAGGAGAAAG 
CGTAAGGTGC AGATCCCGTC AGGCCGGCCG GGTGAACGCG AGACTATGTC AGAGCATGAC 
GAGGACGACG ACAGCTACGA GGATGAGATC TACGAGTCCC GGAGCGCGCG AAGCGGACCC 
AGCGCCTACA GTGGAGGTGG AGGCGGCGGC GGAGGTGGAG GAGCCTCGGG ACGGAGGAGC 
GGCAGAGGCG ACAGGCTGAG TGGGAGGAGG GAGCGGGACA GAGAGCGCAG CGGCTCCAGA 
GAGAGGAGTC TGTCCCCGCG CTCGGACCGC CACTCTGTGT CCTCCAACCT CCCCCCACGG 
CCAGCCAAAG TCACACTCGT CAAATCCCGC AAGAATGAAG CATGTATGGC GTCAGGAAGT 
TCTCAGAAGG TGACATCAGC AGAATACGGG CTTCGCCTGG CCAGCCACAT CTTCGTGAAG 
GACATTTCCC CCGAGAGCCT GGCAGCCCGA GACGGAAACA TCCAAGAGGG AGACGTGGTG 
CTTAAGATTA ATGGCACGGT AACAGAGAAT TTGTCCCTGA TCGATGCTAA GAAGCTGATC 
GAGAGGTCAA AGGGAAAGCT GAAGATGGTG GTGCAGAGAG ACGAGCGGGC GACGCTGCTG 
AACATCCCCG ATCTGGACGA CAGCATACCT TCTGCTAATG CCTCGGACAG AGATGATATT 
TCAGACATCC ACTCCGTGGC CTCTGATCAT TCCAACCGAT CCCACGACCG GAAGAGGAGC 
AGCCGCTCTC GTTCTCCAGA CCGCCGCTCC GAACCCTCAG ACCACTCCAG ACACTCTCCA 
CCACAGATCA GCAACGGCAG GGCCACAGGG CCGACGGCTC AGAGAACGCG GCCAGTTCAC 
AGGAGCCGTG ATGAAGACAG GATCTCCAAG GCCGGGCCGC TTCCTCTACC TGCTAAGATG 
GTGGAGGAGA TGCCGAAAGA GCAGAGCGCA GCCAGAGAAA CAGAGAAACA GCTCCCCCCT 
CTGCCAGAGC CCAAACCTGT GTACGCTCAG CCGGGCCAGC CGGACGTGGA CCTGCCTGTT 
AGTCCGGCAG ATGCTCCTGT GCCCAGCGTC ACACATGATG ACAGCATCTT ACGGCCGAGC 
ATGAAGCTGG TGAAGTTCAA GAAGGGCGAG AGCGTGGGCT TGAGGCTCGC TGGGGGGAAC 
GACGTTGGGA TATTTGTGGC GGGAGTTCTG GAGGACAGTC CTGCTGCTAA AGAGGGCCTG 
GAAGAGGGAG ACCAGATTCT CAGGGTAAAT AATGTGGATT TTGCAAATAT CATCCGAGAG 
GAGGCAGTGC TGTTCCTGCT GGATCTGCCC AAAGGAGAGG AGGTCACCAT CCTGGCCCAG 
AAGAAGAAAG ACGTGTACCG CCGCATTGTA GAGTCTGACG TGGGAGACTC GTTCTACATC 
AGGACTCATT TTGAATATGA GAAGGAGTCG CCCTATGGCC TGAGCTTCAA TAAGGGGGAG 
GTCTTCAGAG TCGTCGACAC CCTTTACAAT GGCAAACTGG GCTCCTGGCT CGCCATCCGC 
ATCGGCAAAA ACCACCAGGA GGTGGAGCGG GGCATCATCC CCAACAAGAA CAGGGCTGAG 
CAGTTATCAA GTGTCCAGTA CACTCTCCCA AAGACTCCAG GAGGGGATCG CGCCGACTTC 
TGGAGATTCA GAGGCCTCCG CAGCTCCAAA CGAAACCTGA GGAAGAGCAG AGAAGACCTG 
TCTGCTCAGC CGGTCCAAAC CAAATTCCCT GCTTATGAAA GAGTGGTGCT TAGAGAAGCT 
GGTTTCTTGA GGCCAGTGGT CATTTTCGGA CCGATCGCGG ATGTGGCTAG AGAGAAGCTG 
TCCAGAGAGG AGACTGACCT CTTCGAACTT GCAAAGAGTG AACCCAGAGA TGCGGGCACA 
GACCAGCGCA GCTCCGGAAT CATCCGTCTC CATACCATTA AACAGATCAT TGACCGAGAC 
AAGCACGCGG TGCTGGACAT CACCCCTAAT GCGGTGGACC GTCTGAACTA CGCTCAGTGG 
TACCCCATCG TGGTGTTTCT GAATCCAGAC AGCAAACAGG GCGTCAAGAA CATGAGGACC 
AGGCTCTGTC CCGAGTCCAG GAAGAGCGCC CGAAAGCTCT ACGAGCGTGC TCTCAAACTG 
AGGAAGAACA ACCATCACCT TTTCACCACC ACTATTAACT TGAACAACAT GAATGACGGG 
TGGTACGGTG CTTTGAAAGA GAGCATCCAG CAGCAGCAGA ACCAGTTGGT CTGGGTCTCA 
GAGGGCAAGG CCGACGGCGC TCCTGACGAT GACCTTGACA TCCATGACGA CCGCCTCTCC 
TACCTCTCGG CTCCGGGCAG CGAGTACAGC ATGTACAGCA CCGACAGCCG CCACACGTCC 
GACTATGAGG ACACCGACAC GGAGGGTGGT GCCTACACCG ACCAGGAGCT GGACGAGACC 
ATGAACGATG AGGTGGGCCT GCCTAGCGAG CCTGCCATCA CACGCTCATC TGAGCCTGTG 
CGAGAGGACC CGCCAGTCAT TCAGGACGCA GCTGGATACC CCAGCTACCA GCATGCGGTA 
CCGCAACCTG AAGCGGTGAG CCGCATTGAC CCGGCCGGGT TTAAGATGGC AGCGCCGCAG 
CAGCAAGCTG AGGCTGCTCT GGCCTCGCCG CCCCCTCCCT CTGCAGCGGT CCAGCCCACC 
GCAACACTAG CGGGTACGAA CCCCGAGGAG CCGCCTGGCA CCCCTCAGGC CGACTCCCTT 
AACAGCCCCG TCCCTGTTCC CGACCCCCAG CCCGAACCTG AGCTCGCTCA GCCCCCAACA 
CACGACCCCC ACCAGTCGCT TCCTCCTGGC CCAGATCCAA AGATGTATAA AAAGGATCTG 
TACAGCATGG ACGAGCCGGT GCGAGTGACC CACGGCGTGA AGCCTCCCCC TCCCCAGCAG 
CAGCAGCAGC CTTTAGGCCC CCCAACCTCG CTCTCCTACA GCCACCAGCC TGTCTACCAG 
GACCAACAGC CATACCGCCA GTACGACCAC CCGCCTTATG GCTATGACGG TGGCGGCTAC 
GCACAACCAA AGCCTCACAA CTACGACCCG CACCTGCACT ACGACAACCG TGTGCCTCAC 
TATAACGAGC AGTGGCCCAC GTACGACCAG CAGCAGCAGC AGCAGACTTC CCCCCAGCCG 
CCACCTGCGT CCGGCTTCCC TCAGGGCCAC CAGCCTCCGC CTCCACCACC GATGGGATAT 
GAGCCACGCT CCCCATACGA GGACGGACCT AACAGGGATT TCAGCCCTCC TCAGTCCCAG 
TATGACGGTG TGTCCCCAAT GGGCTACGAT CACCGGCCGC GGCACCCCAA ACCTGCACCG 
GTCCGATATG AAGAGCCTCC ACCTCCACCT CCTCCGGTGT CCTACGATGC ACGGTCACCT 
TTCGAACCCG AGCCGCATGC TTTCCCAGGC AACACACATC GCTCGCCAGA CCCACCGAAA 
CAGTATTATG GAGACGCCGC TATGAGGCCC TTGTACAACC CTGGACCTCC GAACAGAGTG 
TACAAACATG AGCCGGTGAA CTCTGAGCCT CCTGTACCGC CTCCCAAACC TGAATCTGTC 
CTGTCTCCAG GAGAACCGCC CCATTCTGCA GCTTCCAAAC CATTGCCGCC TCCTCCCAGA 
GATGATGACG ATGATGATGA CGATGATGAT GACCCTGCCA TGAAGCCCCA GTCTGTGCTC 
AACAGGGTCA AAATGTTCGA AAACAAACGC TCTGTGTCGG TAGACCGAGC CAAGGACGCA 
CCTGACGTCA CTGGAATTAG GCCCACAGAT CTCCCCAAAC CTGCGAGCAC CCCTGGACCT 
GTGCTCAAAG CCAACTCTCT CAGCAACCTG GAGCAGGACA AACCCTCCTA CAGAGCTCCA 
GAGCCGCAGA AACCTCAGAC GCGTGTTGGG GACGATGTGG TTCGCTCCAA CCACTACGAC 
CCTGATGATG ATGAAGAGTA CTACAGAAAA CAGCTCTCAT TTTTCGACCG CCGCAGTTTC 
GACAGTAAGG TCATGAATCC ACCCAGCGCT GGAGTAAACC GCTTCCATGA GCCGCCCAAA 
CCACCCCAGA CACAGATCGC TTATCCTTTC GCTAGGACGG AGTCTGTGGA GAAGGTGAGT 
CCTGTGGACA AGAGATACGA GCCTCTGCCG CCAGTCAACC CTTCACCTGC TCCATACAGC 
CAGAACACAC CTGTCGCTCC GCCCACATCC CTGCCCAAAC TCACCAACAT CGAGGTGAAC 
TCTCTGCCAG ATCCTCACAG CTCTCCCAAA GCCAAACCGG ACCTGTCTGC TCTGAGAGCT 
CCGACCCGGG ACGAGCCCAT CCAGAGCAGC TACTTGCCCC CCAAGTCCAC CATCAATGGC 
ATGGATGCTC CTCCCAAAAC ACTCGGCGTG CCGACCAGCT ACAACCGCTA CGTCCCCAAG 
CCTTACACCA GCTCGGCGCG ACCCTTCGAG CGCAAGTTCG AGAGTCCGAA ATTCAACCAC 
AACCTGCTTC CCAACGACAC GCAGTCCAAA CCCAGTGTCA ACAACAACCT GAAGCCTCAG 
ATCTCCCCAC AACCCCAGGA CACGGACAGC GGCGTCGACA CCTTCACTCG CACCATGGAC 
AACAGGCCAA AATATCAGCA CAACAACATC AACGCCATTC CCAAAGCCGT CCCAGTCAGC 
CCCAGTGCAC TGGATGATGA TGAAGAAGAT GAGGGACACA CAGTTGTGGC CACGGCGCGG 
GGCATCTTTA ACTGTAATGG CGGGGTGTTG AGCTCTATAG AGACGGGCGT CAGCATCATT 
ATCCCACAGG GAGCCATTCC TGATAGTGTG GAGCAGGAGA TCTACTTCAA AGTGTGCCGG 
GACAACAGCA TCCTGCCTCC TCTGGATAAG GAGAAAGGTG AAACTCTGCT GAGTCCGCTG 
GTGATGTGTG GTCCTCACGG CCTGAAGTTC CTGAAGCCTG TGGAGCTGCG CTTGCCACAC 
TGTGCGTCTA TGACCCCTGA TGGTTGGTCT TTTGCTCTAA AATCCTCCGA CTCCTCGTCG 
GGTGACCCCA AATGCTGGCA AAACAAGTGT CTACCAGGAG ACCCCAATTA CCTGGTGGGC 
GCAAACTGTG TGTCTGTGCT GATCGACCAC TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp1b ( XM_021470736.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326411.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X18, mRNA.

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CloneID	ODa44629
Clone ID Related Accession (Same CDS sequence)	XM_021470736.1
Accession Version	XM_021470736.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5403bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tight junction protein ZO-1 isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCCC GGGCTGCCTC TAATAAGAGT GCAGCAATGG ATGAGACGGT AATATGGGAA 
CAGCACACTG TAACTCTACA CAGGGCTCCA GGGTTTGGCT TCGGGATCGC CATCTCCGGC 
GGACGGGACA ACCCTCACTT CCAGAGCGGG GAGACGTCCA TCGTCATATC AGATGTGCTG 
AAGGGAGGCC CCGCCGAGGG CCTGCTGCAG GAGAATGACA GAGTGGTGAT GGTCAACGCC 
GTCTCCATGG ACAATGTGGA TCACGCTTAC GCAGTGCAGC AGCTCCGCAA AAGTGGCAAG 
AATGCAAAAA TAACCATCAG GAGAAAGCGT AAGGTGCAGA TCCCGTCAGG CCGGCCGGGT 
GAACGCGAGA CTATGTCAGA GCATGACGAG GACGACGACA GCTACGAGGA TGAGATCTAC 
GAGTCCCGGA GCGCGCGAAG CGGACCCAGC GCCTACAGTG GAGGTGGAGG CGGCGGCGGA 
GGTGGAGGAG CCTCGGGACG GAGGAGCGGC AGAGGCGACA GGCTGAGTGG GAGGAGGGAG 
CGGGACAGAG AGCGCAGCGG CTCCAGAGAG AGGAGTCTGT CCCCGCGCTC GGACCGCCAC 
TCTGTGTCCT CCAACCTCCC CCCACGGCCA GCCAAAGTCA CACTCGTCAA ATCCCGCAAG 
AATGAAGCAT GTATGGCGTC AGGAAGTTCT CAGAAGGTGA CATCAGCAGA ATACGGGCTT 
CGCCTGGCCA GCCACATCTT CGTGAAGGAC ATTTCCCCCG AGAGCCTGGC AGCCCGAGAC 
GGAAACATCC AAGAGGGAGA CGTGGTGCTT AAGATTAATG GCACGGTAAC AGAGAATTTG 
TCCCTGATCG ATGCTAAGAA GCTGATCGAG AGGTCAAAGG GAAAGCTGAA GATGGTGGTG 
CAGAGAGACG AGCGGGCGAC GCTGCTGAAC ATCCCCGATC TGGACGACAG CATACCTTCT 
GCTAATGCCT CGGACAGAGA TGATATTTCA GACATCCACT CCGTGGCCTC TGATCATTCC 
AACCGATCCC ACGACCGGAA GAGGAGCAGC CGCTCTCGTT CTCCAGACCG CCGCTCCGAA 
CCCTCAGACC ACTCCAGACA CTCTCCACCA CAGATCAGCA ACGGCAGGGC CACAGGGCCG 
ACGGCTCAGA GAACGCGGCC AGTTCACAGG AGCCGTGATG AAGACAGGAT CTCCAAGGCC 
GGGCCGCTTC CTCTACCTGC TAAGATGGTG GAGGAGATGC CGAAAGAGCA GAGCGCAGCC 
AGAGAAACAG AGAAACAGCT CCCCCCTCTG CCAGAGCCCA AACCTGTGTA CGCTCAGCCG 
GGCCAGCCGG ACGTGGACCT GCCTGTTAGT CCGGCAGATG CTCCTGTGCC CAGCGTCACA 
CATGATGACA GCATCTTACG GCCGAGCATG AAGCTGGTGA AGTTCAAGAA GGGCGAGAGC 
GTGGGCTTGA GGCTCGCTGG GGGGAACGAC GTTGGGATAT TTGTGGCGGG AGTTCTGGAG 
GACAGTCCTG CTGCTAAAGA GGGCCTGGAA GAGGGAGACC AGATTCTCAG GGTAAATAAT 
GTGGATTTTG CAAATATCAT CCGAGAGGAG GCAGTGCTGT TCCTGCTGGA TCTGCCCAAA 
GGAGAGGAGG TCACCATCCT GGCCCAGAAG AAGAAAGACG TGTACCGCCG CATTGTAGAG 
TCTGACGTGG GAGACTCGTT CTACATCAGG ACTCATTTTG AATATGAGAA GGAGTCGCCC 
TATGGCCTGA GCTTCAATAA GGGGGAGGTC TTCAGAGTCG TCGACACCCT TTACAATGGC 
AAACTGGGCT CCTGGCTCGC CATCCGCATC GGCAAAAACC ACCAGGAGGT GGAGCGGGGC 
ATCATCCCCA ACAAGAACAG GGCTGAGCAG TTATCAAGTG TCCAGTACAC TCTCCCAAAG 
ACTCCAGGAG GGGATCGCGC CGACTTCTGG AGATTCAGAG GCCTCCGCAG CTCCAAACGA 
AACCTGAGGA AGAGCAGAGA AGACCTGTCT GCTCAGCCGG TCCAAACCAA ATTCCCTGCT 
TATGAAAGAG TGGTGCTTAG AGAAGCTGGT TTCTTGAGGC CAGTGGTCAT TTTCGGACCG 
ATCGCGGATG TGGCTAGAGA GAAGCTGTCC AGAGAGGAGA CTGACCTCTT CGAACTTGCA 
AAGAGTGAAC CCAGAGATGC GGGCACAGAC CAGCGCAGCT CCGGAATCAT CCGTCTCCAT 
ACCATTAAAC AGATCATTGA CCGAGACAAG CACGCGGTGC TGGACATCAC CCCTAATGCG 
GTGGACCGTC TGAACTACGC TCAGTGGTAC CCCATCGTGG TGTTTCTGAA TCCAGACAGC 
AAACAGGGCG TCAAGAACAT GAGGACCAGG CTCTGTCCCG AGTCCAGGAA GAGCGCCCGA 
AAGCTCTACG AGCGTGCTCT CAAACTGAGG AAGAACAACC ATCACCTTTT CACCACCACT 
ATTAACTTGA ACAACATGAA TGACGGGTGG TACGGTGCTT TGAAAGAGAG CATCCAGCAG 
CAGCAGAACC AGTTGGTCTG GGTCTCAGAG GGCAAGGCCG ACGGCGCTCC TGACGATGAC 
CTTGACATCC ATGACGACCG CCTCTCCTAC CTCTCGGCTC CGGGCAGCGA GTACAGCATG 
TACAGCACCG ACAGCCGCCA CACGTCCGAC TATGAGGACA CCGACACGGA GGGTGGTGCC 
TACACCGACC AGGAGCTGGA CGAGACCATG AACGATGAGG TGGGCCTGCC TAGCGAGCCT 
GCCATCACAC GCTCATCTGA GCCTGTGCGA GAGGACCCGC CAGTCATTCA GGACGCAGCT 
GGATACCCCA GCTACCAGCA TGCGGTACCG CAACCTGAAG CGGTGAGCCG CATTGACCCG 
GCCGGGTTTA AGATGGCAGC GCCGCAGCAG CAAGCTGAGG CTGCTCTGGC CTCGCCGCCC 
CCTCCCTCTG CAGCGGTCCA GCCCACCGCA ACACTAGCGG GTACGAACCC CGAGGAGCCG 
CCTGGCACCC CTCAGGCCGA CTCCCTTAAC AGCCCCGTCC CTGTTCCCGA CCCCCAGCCC 
GAACCTGAGC TCGCTCAGCC CCCAACACAC GACCCCCACC AGTCGCTTCC TCCTGGCCCA 
GATCCAAAGA TGTATAAAAA GGATCTGTAC AGCATGGACG AGCCGGTGCG AGTGACCCAC 
GGCGTGAAGC CTCCCCCTCC CCAGCAGCAG CAGCAGCCTT TAGGCCCCCC AACCTCGCTC 
TCCTACAGCC ACCAGCCTGT CTACCAGGAC CAACAGCCAT ACCGCCAGTA CGACCACCCG 
CCTTATGGCT ATGACGGTGG CGGCTACGCA CAACCAAAGC CTCACAACTA CGACCCGCAC 
CTGCACTACG ACAACCGTGT GCCTCACTAT AACGAGCAGT GGCCCACGTA CGACCAGCAG 
CAGCAGCAGC AGACTTCCCC CCAGCCGCCA CCTGCGTCCG GCTTCCCTCA GGGCCACCAG 
CCTCCGCCTC CACCACCGAT GGGATATGAG CCACGCTCCC CATACGAGGA CGGACCTAAC 
AGGGATTTCA GCCCTCCTCA GTCCCAGTAT GACGGTGTGT CCCCAATGGG CTACGATCAC 
CGGCCGCGGC ACCCCAAACC TGCACCGGTC CGATATGAAG AGCCTCCACC TCCACCTCCT 
CCGGTGTCCT ACGATGCACG GTCACCTTTC GAACCCGAGC CGCATGCTTT CCCAGGCAAC 
ACACATCGCT CGCCAGACCC ACCGAAACAG TATTATGGAG ACGCCGCTAT GAGGCCCTTG 
TACAACCCTG GACCTCCGAA CAGAGTGTAC AAACATGAGC CGGTGAACTC TGAGCCTCCT 
GTACCGCCTC CCAAACCTGA ATCTGTCCTG TCTCCAGGAG AACCGCCCCA TTCTGCAGCT 
TCCAAACCAT TGCCGCCTCC TCCCAGAGAT GATGACGATG ATGATGACGA TGATGATGAC 
CCTGCCATGA AGCCCCAGTC TGTGCTCAAC AGGGTCAAAA TGTTCGAAAA CAAACGCTCT 
GTGTCGGTAG ACCGAGCCAA GGACGCACCT GACGTCACTG GAATTAGGCC CACAGATCTC 
CCCAAACCTG CGAGCACCCC TGGACCTGTG CTCAAAGCCA ACTCTCTCAG CAACCTGGAG 
CAGGACAAAC CCTCCTACAG AGCTCCAGAG CCGCAGAAAC CTCAGACGCG TGTTGGGGAC 
GATGTGGTTC GCTCCAACCA CTACGACCCT GATGATGATG AAGAGTACTA CAGAAAACAG 
CTCTCATTTT TCGACCGCCG CAGTTTCGAC AGTAAGGTCA TGAATCCACC CAGCGCTGGA 
GTAAACCGCT TCCATGAGCC GCCCAAACCA CCCCAGACAC AGATCGCTTA TCCTTTCGCT 
AGGACGGAGT CTGTGGAGAA GGTGAGTCCT GTGGACAAGA GATACGAGCC TCTGCCGCCA 
GTCAACCCTT CACCTGCTCC ATACAGCCAG AACACACCTG TCGCTCCGCC CACATCCCTG 
CCCAAACTCA CCAACATCGA GGTGAACTCT CTGCCAGATC CTCACAGCTC TCCCAAAGCC 
AAACCGGACC TGTCTGCTCT GAGAGCTCCG ACCCGGGACG AGCCCATCCA GAGCAGCTAC 
TTGCCCCCCA AGTCCACCAT CAATGGCATG GATGCTCCTC CCAAAACACT CGGCGTGCCG 
ACCAGCTACA ACCGCTACGT CCCCAAGCCT TACACCAGCT CGGCGCGACC CTTCGAGCGC 
AAGTTCGAGA GTCCGAAATT CAACCACAAC CTGCTTCCCA ACGACACGCA GTCCAAACCC 
AGTGTCAACA ACAACCTGAA GCCTCAGATC TCCCCACAAC CCCAGGACAC GGACAGCGGC 
GTCGACACCT TCACTCGCAC CATGGACAAC AGGCCAAAAT ATCAGCACAA CAACATCAAC 
GCCATTCCCA AAGCCGTCCC AGTCAGCCCC AGTGCACTGG ATGATGATGA AGAAGATGAG 
GGACACACAG TTGTGGCCAC GGCGCGGGGC ATCTTTAACT GTAATGGCGG GGTGTTGAGC 
TCTATAGAGA CGGGCGTCAG CATCATTATC CCACAGGGAG CCATTCCTGA TAGTGTGGAG 
CAGGAGATCT ACTTCAAAGT GTGCCGGGAC AACAGCATCC TGCCTCCTCT GGATAAGGAG 
AAAGGTGAAA CTCTGCTGAG TCCGCTGGTG ATGTGTGGTC CTCACGGCCT GAAGTTCCTG 
AAGCCTGTGG AGCTGCGCTT GCCACACTGT GCGTCTATGA CCCCTGATGG TTGGTCTTTT 
GCTCTAAAAT CCTCCGACTC CTCGTCGGGT GACCCCAAAT GCTGGCAAAA CAAGTGTCTA 
CCAGGAGACC CCAATTACCT GGTGGGCGCA AACTGTGTGT CTGTGCTGAT CGACCACTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326411.1
CDS	503..5905
Translation	MSARAASNKSAAMDETVIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGLLQENDRVVMVNAVSMDNVDHAYAVQQLRKSGKNAKITIRRKRKVQIPSGRPGERETMSEHDEDDDSYEDEIYESRSARSGPSAYSGGGGGGGGGGASGRRSGRGDRLSGRRERDRERSGSRERSLSPRSDRHSVSSNLPPRPAKVTLVKSRKNEACMASGSSQKVTSAEYGLRLASHIFVKDISPESLAARDGNIQEGDVVLKINGTVTENLSLIDAKKLIERSKGKLKMVVQRDERATLLNIPDLDDSIPSANASDRDDISDIHSVASDHSNRSHDRKRSSRSRSPDRRSEPSDHSRHSPPQISNGRATGPTAQRTRPVHRSRDEDRISKAGPLPLPAKMVEEMPKEQSAARETEKQLPPLPEPKPVYAQPGQPDVDLPVSPADAPVPSVTHDDSILRPSMKLVKFKKGESVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFANIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHQEVERGIIPNKNRAEQLSSVQYTLPKTPGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVVIFGPIADVAREKLSREETDLFELAKSEPRDAGTDQRSSGIIRLHTIKQIIDRDKHAVLDITPNAVDRLNYAQWYPIVVFLNPDSKQGVKNMRTRLCPESRKSARKLYERALKLRKNNHHLFTTTINLNNMNDGWYGALKESIQQQQNQLVWVSEGKADGAPDDDLDIHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETMNDEVGLPSEPAITRSSEPVREDPPVIQDAAGYPSYQHAVPQPEAVSRIDPAGFKMAAPQQQAEAALASPPPPSAAVQPTATLAGTNPEEPPGTPQADSLNSPVPVPDPQPEPELAQPPTHDPHQSLPPGPDPKMYKKDLYSMDEPVRVTHGVKPPPPQQQQQPLGPPTSLSYSHQPVYQDQQPYRQYDHPPYGYDGGGYAQPKPHNYDPHLHYDNRVPHYNEQWPTYDQQQQQQTSPQPPPASGFPQGHQPPPPPPMGYEPRSPYEDGPNRDFSPPQSQYDGVSPMGYDHRPRHPKPAPVRYEEPPPPPPPVSYDARSPFEPEPHAFPGNTHRSPDPPKQYYGDAAMRPLYNPGPPNRVYKHEPVNSEPPVPPPKPESVLSPGEPPHSAASKPLPPPPRDDDDDDDDDDDPAMKPQSVLNRVKMFENKRSVSVDRAKDAPDVTGIRPTDLPKPASTPGPVLKANSLSNLEQDKPSYRAPEPQKPQTRVGDDVVRSNHYDPDDDEEYYRKQLSFFDRRSFDSKVMNPPSAGVNRFHEPPKPPQTQIAYPFARTESVEKVSPVDKRYEPLPPVNPSPAPYSQNTPVAPPTSLPKLTNIEVNSLPDPHSSPKAKPDLSALRAPTRDEPIQSSYLPPKSTINGMDAPPKTLGVPTSYNRYVPKPYTSSARPFERKFESPKFNHNLLPNDTQSKPSVNNNLKPQISPQPQDTDSGVDTFTRTMDNRPKYQHNNINAIPKAVPVSPSALDDDEEDEGHTVVATARGIFNCNGGVLSSIETGVSIIIPQGAIPDSVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKCWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_021470736.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tight junction protein 1b (tjp1b), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470736.1

ORF Insert Sequence:

ATGTCTGCCC GGGCTGCCTC TAATAAGAGT GCAGCAATGG ATGAGACGGT AATATGGGAA 
CAGCACACTG TAACTCTACA CAGGGCTCCA GGGTTTGGCT TCGGGATCGC CATCTCCGGC 
GGACGGGACA ACCCTCACTT CCAGAGCGGG GAGACGTCCA TCGTCATATC AGATGTGCTG 
AAGGGAGGCC CCGCCGAGGG CCTGCTGCAG GAGAATGACA GAGTGGTGAT GGTCAACGCC 
GTCTCCATGG ACAATGTGGA TCACGCTTAC GCAGTGCAGC AGCTCCGCAA AAGTGGCAAG 
AATGCAAAAA TAACCATCAG GAGAAAGCGT AAGGTGCAGA TCCCGTCAGG CCGGCCGGGT 
GAACGCGAGA CTATGTCAGA GCATGACGAG GACGACGACA GCTACGAGGA TGAGATCTAC 
GAGTCCCGGA GCGCGCGAAG CGGACCCAGC GCCTACAGTG GAGGTGGAGG CGGCGGCGGA 
GGTGGAGGAG CCTCGGGACG GAGGAGCGGC AGAGGCGACA GGCTGAGTGG GAGGAGGGAG 
CGGGACAGAG AGCGCAGCGG CTCCAGAGAG AGGAGTCTGT CCCCGCGCTC GGACCGCCAC 
TCTGTGTCCT CCAACCTCCC CCCACGGCCA GCCAAAGTCA CACTCGTCAA ATCCCGCAAG 
AATGAAGCAT GTATGGCGTC AGGAAGTTCT CAGAAGGTGA CATCAGCAGA ATACGGGCTT 
CGCCTGGCCA GCCACATCTT CGTGAAGGAC ATTTCCCCCG AGAGCCTGGC AGCCCGAGAC 
GGAAACATCC AAGAGGGAGA CGTGGTGCTT AAGATTAATG GCACGGTAAC AGAGAATTTG 
TCCCTGATCG ATGCTAAGAA GCTGATCGAG AGGTCAAAGG GAAAGCTGAA GATGGTGGTG 
CAGAGAGACG AGCGGGCGAC GCTGCTGAAC ATCCCCGATC TGGACGACAG CATACCTTCT 
GCTAATGCCT CGGACAGAGA TGATATTTCA GACATCCACT CCGTGGCCTC TGATCATTCC 
AACCGATCCC ACGACCGGAA GAGGAGCAGC CGCTCTCGTT CTCCAGACCG CCGCTCCGAA 
CCCTCAGACC ACTCCAGACA CTCTCCACCA CAGATCAGCA ACGGCAGGGC CACAGGGCCG 
ACGGCTCAGA GAACGCGGCC AGTTCACAGG AGCCGTGATG AAGACAGGAT CTCCAAGGCC 
GGGCCGCTTC CTCTACCTGC TAAGATGGTG GAGGAGATGC CGAAAGAGCA GAGCGCAGCC 
AGAGAAACAG AGAAACAGCT CCCCCCTCTG CCAGAGCCCA AACCTGTGTA CGCTCAGCCG 
GGCCAGCCGG ACGTGGACCT GCCTGTTAGT CCGGCAGATG CTCCTGTGCC CAGCGTCACA 
CATGATGACA GCATCTTACG GCCGAGCATG AAGCTGGTGA AGTTCAAGAA GGGCGAGAGC 
GTGGGCTTGA GGCTCGCTGG GGGGAACGAC GTTGGGATAT TTGTGGCGGG AGTTCTGGAG 
GACAGTCCTG CTGCTAAAGA GGGCCTGGAA GAGGGAGACC AGATTCTCAG GGTAAATAAT 
GTGGATTTTG CAAATATCAT CCGAGAGGAG GCAGTGCTGT TCCTGCTGGA TCTGCCCAAA 
GGAGAGGAGG TCACCATCCT GGCCCAGAAG AAGAAAGACG TGTACCGCCG CATTGTAGAG 
TCTGACGTGG GAGACTCGTT CTACATCAGG ACTCATTTTG AATATGAGAA GGAGTCGCCC 
TATGGCCTGA GCTTCAATAA GGGGGAGGTC TTCAGAGTCG TCGACACCCT TTACAATGGC 
AAACTGGGCT CCTGGCTCGC CATCCGCATC GGCAAAAACC ACCAGGAGGT GGAGCGGGGC 
ATCATCCCCA ACAAGAACAG GGCTGAGCAG TTATCAAGTG TCCAGTACAC TCTCCCAAAG 
ACTCCAGGAG GGGATCGCGC CGACTTCTGG AGATTCAGAG GCCTCCGCAG CTCCAAACGA 
AACCTGAGGA AGAGCAGAGA AGACCTGTCT GCTCAGCCGG TCCAAACCAA ATTCCCTGCT 
TATGAAAGAG TGGTGCTTAG AGAAGCTGGT TTCTTGAGGC CAGTGGTCAT TTTCGGACCG 
ATCGCGGATG TGGCTAGAGA GAAGCTGTCC AGAGAGGAGA CTGACCTCTT CGAACTTGCA 
AAGAGTGAAC CCAGAGATGC GGGCACAGAC CAGCGCAGCT CCGGAATCAT CCGTCTCCAT 
ACCATTAAAC AGATCATTGA CCGAGACAAG CACGCGGTGC TGGACATCAC CCCTAATGCG 
GTGGACCGTC TGAACTACGC TCAGTGGTAC CCCATCGTGG TGTTTCTGAA TCCAGACAGC 
AAACAGGGCG TCAAGAACAT GAGGACCAGG CTCTGTCCCG AGTCCAGGAA GAGCGCCCGA 
AAGCTCTACG AGCGTGCTCT CAAACTGAGG AAGAACAACC ATCACCTTTT CACCACCACT 
ATTAACTTGA ACAACATGAA TGACGGGTGG TACGGTGCTT TGAAAGAGAG CATCCAGCAG 
CAGCAGAACC AGTTGGTCTG GGTCTCAGAG GGCAAGGCCG ACGGCGCTCC TGACGATGAC 
CTTGACATCC ATGACGACCG CCTCTCCTAC CTCTCGGCTC CGGGCAGCGA GTACAGCATG 
TACAGCACCG ACAGCCGCCA CACGTCCGAC TATGAGGACA CCGACACGGA GGGTGGTGCC 
TACACCGACC AGGAGCTGGA CGAGACCATG AACGATGAGG TGGGCCTGCC TAGCGAGCCT 
GCCATCACAC GCTCATCTGA GCCTGTGCGA GAGGACCCGC CAGTCATTCA GGACGCAGCT 
GGATACCCCA GCTACCAGCA TGCGGTACCG CAACCTGAAG CGGTGAGCCG CATTGACCCG 
GCCGGGTTTA AGATGGCAGC GCCGCAGCAG CAAGCTGAGG CTGCTCTGGC CTCGCCGCCC 
CCTCCCTCTG CAGCGGTCCA GCCCACCGCA ACACTAGCGG GTACGAACCC CGAGGAGCCG 
CCTGGCACCC CTCAGGCCGA CTCCCTTAAC AGCCCCGTCC CTGTTCCCGA CCCCCAGCCC 
GAACCTGAGC TCGCTCAGCC CCCAACACAC GACCCCCACC AGTCGCTTCC TCCTGGCCCA 
GATCCAAAGA TGTATAAAAA GGATCTGTAC AGCATGGACG AGCCGGTGCG AGTGACCCAC 
GGCGTGAAGC CTCCCCCTCC CCAGCAGCAG CAGCAGCCTT TAGGCCCCCC AACCTCGCTC 
TCCTACAGCC ACCAGCCTGT CTACCAGGAC CAACAGCCAT ACCGCCAGTA CGACCACCCG 
CCTTATGGCT ATGACGGTGG CGGCTACGCA CAACCAAAGC CTCACAACTA CGACCCGCAC 
CTGCACTACG ACAACCGTGT GCCTCACTAT AACGAGCAGT GGCCCACGTA CGACCAGCAG 
CAGCAGCAGC AGACTTCCCC CCAGCCGCCA CCTGCGTCCG GCTTCCCTCA GGGCCACCAG 
CCTCCGCCTC CACCACCGAT GGGATATGAG CCACGCTCCC CATACGAGGA CGGACCTAAC 
AGGGATTTCA GCCCTCCTCA GTCCCAGTAT GACGGTGTGT CCCCAATGGG CTACGATCAC 
CGGCCGCGGC ACCCCAAACC TGCACCGGTC CGATATGAAG AGCCTCCACC TCCACCTCCT 
CCGGTGTCCT ACGATGCACG GTCACCTTTC GAACCCGAGC CGCATGCTTT CCCAGGCAAC 
ACACATCGCT CGCCAGACCC ACCGAAACAG TATTATGGAG ACGCCGCTAT GAGGCCCTTG 
TACAACCCTG GACCTCCGAA CAGAGTGTAC AAACATGAGC CGGTGAACTC TGAGCCTCCT 
GTACCGCCTC CCAAACCTGA ATCTGTCCTG TCTCCAGGAG AACCGCCCCA TTCTGCAGCT 
TCCAAACCAT TGCCGCCTCC TCCCAGAGAT GATGACGATG ATGATGACGA TGATGATGAC 
CCTGCCATGA AGCCCCAGTC TGTGCTCAAC AGGGTCAAAA TGTTCGAAAA CAAACGCTCT 
GTGTCGGTAG ACCGAGCCAA GGACGCACCT GACGTCACTG GAATTAGGCC CACAGATCTC 
CCCAAACCTG CGAGCACCCC TGGACCTGTG CTCAAAGCCA ACTCTCTCAG CAACCTGGAG 
CAGGACAAAC CCTCCTACAG AGCTCCAGAG CCGCAGAAAC CTCAGACGCG TGTTGGGGAC 
GATGTGGTTC GCTCCAACCA CTACGACCCT GATGATGATG AAGAGTACTA CAGAAAACAG 
CTCTCATTTT TCGACCGCCG CAGTTTCGAC AGTAAGGTCA TGAATCCACC CAGCGCTGGA 
GTAAACCGCT TCCATGAGCC GCCCAAACCA CCCCAGACAC AGATCGCTTA TCCTTTCGCT 
AGGACGGAGT CTGTGGAGAA GGTGAGTCCT GTGGACAAGA GATACGAGCC TCTGCCGCCA 
GTCAACCCTT CACCTGCTCC ATACAGCCAG AACACACCTG TCGCTCCGCC CACATCCCTG 
CCCAAACTCA CCAACATCGA GGTGAACTCT CTGCCAGATC CTCACAGCTC TCCCAAAGCC 
AAACCGGACC TGTCTGCTCT GAGAGCTCCG ACCCGGGACG AGCCCATCCA GAGCAGCTAC 
TTGCCCCCCA AGTCCACCAT CAATGGCATG GATGCTCCTC CCAAAACACT CGGCGTGCCG 
ACCAGCTACA ACCGCTACGT CCCCAAGCCT TACACCAGCT CGGCGCGACC CTTCGAGCGC 
AAGTTCGAGA GTCCGAAATT CAACCACAAC CTGCTTCCCA ACGACACGCA GTCCAAACCC 
AGTGTCAACA ACAACCTGAA GCCTCAGATC TCCCCACAAC CCCAGGACAC GGACAGCGGC 
GTCGACACCT TCACTCGCAC CATGGACAAC AGGCCAAAAT ATCAGCACAA CAACATCAAC 
GCCATTCCCA AAGCCGTCCC AGTCAGCCCC AGTGCACTGG ATGATGATGA AGAAGATGAG 
GGACACACAG TTGTGGCCAC GGCGCGGGGC ATCTTTAACT GTAATGGCGG GGTGTTGAGC 
TCTATAGAGA CGGGCGTCAG CATCATTATC CCACAGGGAG CCATTCCTGA TAGTGTGGAG 
CAGGAGATCT ACTTCAAAGT GTGCCGGGAC AACAGCATCC TGCCTCCTCT GGATAAGGAG 
AAAGGTGAAA CTCTGCTGAG TCCGCTGGTG ATGTGTGGTC CTCACGGCCT GAAGTTCCTG 
AAGCCTGTGG AGCTGCGCTT GCCACACTGT GCGTCTATGA CCCCTGATGG TTGGTCTTTT 
GCTCTAAAAT CCTCCGACTC CTCGTCGGGT GACCCCAAAT GCTGGCAAAA CAAGTGTCTA 
CCAGGAGACC CCAATTACCT GGTGGGCGCA AACTGTGTGT CTGTGCTGAT CGACCACTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp1b ( XM_021470737.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326412.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X19, mRNA.

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CloneID	ODa44630
Clone ID Related Accession (Same CDS sequence)	XM_021470737.1
Accession Version	XM_021470737.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5367bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tight junction protein ZO-1 isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGA CGGTAATATG GGAACAGCAC ACTGTAACTC TACACAGGGC TCCAGGGTTT 
GGCTTCGGGA TCGCCATCTC CGGCGGACGG GACAACCCTC ACTTCCAGAG CGGGGAGACG 
TCCATCGTCA TATCAGATGT GCTGAAGGGA GGCCCCGCCG AGGGCCTGCT GCAGGAGAAT 
GACAGAGTGG TGATGGTCAA CGCCGTCTCC ATGGACAATG TGGATCACGC TTACGCAGTG 
CAGCAGCTCC GCAAAAGTGG CAAGAATGCA AAAATAACCA TCAGGAGAAA GCGTAAGGTG 
CAGATCCCGT CAGGCCGGCC GGGTGAACGC GAGACTATGT CAGAGCATGA CGAGGACGAC 
GACAGCTACG AGGATGAGAT CTACGAGTCC CGGAGCGCGC GAAGCGGACC CAGCGCCTAC 
AGTGGAGGTG GAGGCGGCGG CGGAGGTGGA GGAGCCTCGG GACGGAGGAG CGGCAGAGGC 
GACAGGCTGA GTGGGAGGAG GGAGCGGGAC AGAGAGCGCA GCGGCTCCAG AGAGAGGAGT 
CTGTCCCCGC GCTCGGACCG CCACTCTGTG TCCTCCAACC TCCCCCCACG GCCAGCCAAA 
GTCACACTCG TCAAATCCCG CAAGAATGAA GCATGTATGG CGTCAGGAAG TTCTCAGAAG 
GTGACATCAG CAGAATACGG GCTTCGCCTG GCCAGCCACA TCTTCGTGAA GGACATTTCC 
CCCGAGAGCC TGGCAGCCCG AGACGGAAAC ATCCAAGAGG GAGACGTGGT GCTTAAGATT 
AATGGCACGG TAACAGAGAA TTTGTCCCTG ATCGATGCTA AGAAGCTGAT CGAGAGGTCA 
AAGGGAAAGC TGAAGATGGT GGTGCAGAGA GACGAGCGGG CGACGCTGCT GAACATCCCC 
GATCTGGACG ACAGCATACC TTCTGCTAAT GCCTCGGACA GAGATGATAT TTCAGACATC 
CACTCCGTGG CCTCTGATCA TTCCAACCGA TCCCACGACC GGAAGAGGAG CAGCCGCTCT 
CGTTCTCCAG ACCGCCGCTC CGAACCCTCA GACCACTCCA GACACTCTCC ACCACAGATC 
AGCAACGGCA GGGCCACAGG GCCGACGGCT CAGAGAACGC GGCCAGTTCA CAGGAGCCGT 
GATGAAGACA GGATCTCCAA GGCCGGGCCG CTTCCTCTAC CTGCTAAGAT GGTGGAGGAG 
ATGCCGAAAG AGCAGAGCGC AGCCAGAGAA ACAGAGAAAC AGCTCCCCCC TCTGCCAGAG 
CCCAAACCTG TGTACGCTCA GCCGGGCCAG CCGGACGTGG ACCTGCCTGT TAGTCCGGCA 
GATGCTCCTG TGCCCAGCGT CACACATGAT GACAGCATCT TACGGCCGAG CATGAAGCTG 
GTGAAGTTCA AGAAGGGCGA GAGCGTGGGC TTGAGGCTCG CTGGGGGGAA CGACGTTGGG 
ATATTTGTGG CGGGAGTTCT GGAGGACAGT CCTGCTGCTA AAGAGGGCCT GGAAGAGGGA 
GACCAGATTC TCAGGGTAAA TAATGTGGAT TTTGCAAATA TCATCCGAGA GGAGGCAGTG 
CTGTTCCTGC TGGATCTGCC CAAAGGAGAG GAGGTCACCA TCCTGGCCCA GAAGAAGAAA 
GACGTGTACC GCCGCATTGT AGAGTCTGAC GTGGGAGACT CGTTCTACAT CAGGACTCAT 
TTTGAATATG AGAAGGAGTC GCCCTATGGC CTGAGCTTCA ATAAGGGGGA GGTCTTCAGA 
GTCGTCGACA CCCTTTACAA TGGCAAACTG GGCTCCTGGC TCGCCATCCG CATCGGCAAA 
AACCACCAGG AGGTGGAGCG GGGCATCATC CCCAACAAGA ACAGGGCTGA GCAGTTATCA 
AGTGTCCAGT ACACTCTCCC AAAGACTCCA GGAGGGGATC GCGCCGACTT CTGGAGATTC 
AGAGGCCTCC GCAGCTCCAA ACGAAACCTG AGGAAGAGCA GAGAAGACCT GTCTGCTCAG 
CCGGTCCAAA CCAAATTCCC TGCTTATGAA AGAGTGGTGC TTAGAGAAGC TGGTTTCTTG 
AGGCCAGTGG TCATTTTCGG ACCGATCGCG GATGTGGCTA GAGAGAAGCT GTCCAGAGAG 
GAGACTGACC TCTTCGAACT TGCAAAGAGT GAACCCAGAG ATGCGGGCAC AGACCAGCGC 
AGCTCCGGAA TCATCCGTCT CCATACCATT AAACAGATCA TTGACCGAGA CAAGCACGCG 
GTGCTGGACA TCACCCCTAA TGCGGTGGAC CGTCTGAACT ACGCTCAGTG GTACCCCATC 
GTGGTGTTTC TGAATCCAGA CAGCAAACAG GGCGTCAAGA ACATGAGGAC CAGGCTCTGT 
CCCGAGTCCA GGAAGAGCGC CCGAAAGCTC TACGAGCGTG CTCTCAAACT GAGGAAGAAC 
AACCATCACC TTTTCACCAC CACTATTAAC TTGAACAACA TGAATGACGG GTGGTACGGT 
GCTTTGAAAG AGAGCATCCA GCAGCAGCAG AACCAGTTGG TCTGGGTCTC AGAGGGCAAG 
GCCGACGGCG CTCCTGACGA TGACCTTGAC ATCCATGACG ACCGCCTCTC CTACCTCTCG 
GCTCCGGGCA GCGAGTACAG CATGTACAGC ACCGACAGCC GCCACACGTC CGACTATGAG 
GACACCGACA CGGAGGGTGG TGCCTACACC GACCAGGAGC TGGACGAGAC CATGAACGAT 
GAGGTGGGCC TGCCTAGCGA GCCTGCCATC ACACGCTCAT CTGAGCCTGT GCGAGAGGAC 
CCGCCAGTCA TTCAGGACGC AGCTGGATAC CCCAGCTACC AGCATGCGGT ACCGCAACCT 
GAAGCGGTGA GCCGCATTGA CCCGGCCGGG TTTAAGATGG CAGCGCCGCA GCAGCAAGCT 
GAGGCTGCTC TGGCCTCGCC GCCCCCTCCC TCTGCAGCGG TCCAGCCCAC CGCAACACTA 
GCGGGTACGA ACCCCGAGGA GCCGCCTGGC ACCCCTCAGG CCGACTCCCT TAACAGCCCC 
GTCCCTGTTC CCGACCCCCA GCCCGAACCT GAGCTCGCTC AGCCCCCAAC ACACGACCCC 
CACCAGTCGC TTCCTCCTGG CCCAGATCCA AAGATGTATA AAAAGGATCT GTACAGCATG 
GACGAGCCGG TGCGAGTGAC CCACGGCGTG AAGCCTCCCC CTCCCCAGCA GCAGCAGCAG 
CCTTTAGGCC CCCCAACCTC GCTCTCCTAC AGCCACCAGC CTGTCTACCA GGACCAACAG 
CCATACCGCC AGTACGACCA CCCGCCTTAT GGCTATGACG GTGGCGGCTA CGCACAACCA 
AAGCCTCACA ACTACGACCC GCACCTGCAC TACGACAACC GTGTGCCTCA CTATAACGAG 
CAGTGGCCCA CGTACGACCA GCAGCAGCAG CAGCAGACTT CCCCCCAGCC GCCACCTGCG 
TCCGGCTTCC CTCAGGGCCA CCAGCCTCCG CCTCCACCAC CGATGGGATA TGAGCCACGC 
TCCCCATACG AGGACGGACC TAACAGGGAT TTCAGCCCTC CTCAGTCCCA GTATGACGGT 
GTGTCCCCAA TGGGCTACGA TCACCGGCCG CGGCACCCCA AACCTGCACC GGTCCGATAT 
GAAGAGCCTC CACCTCCACC TCCTCCGGTG TCCTACGATG CACGGTCACC TTTCGAACCC 
GAGCCGCATG CTTTCCCAGG CAACACACAT CGCTCGCCAG ACCCACCGAA ACAGTATTAT 
GGAGACGCCG CTATGAGGCC CTTGTACAAC CCTGGACCTC CGAACAGAGT GTACAAACAT 
GAGCCGGTGA ACTCTGAGCC TCCTGTACCG CCTCCCAAAC CTGAATCTGT CCTGTCTCCA 
GGAGAACCGC CCCATTCTGC AGCTTCCAAA CCATTGCCGC CTCCTCCCAG AGATGATGAC 
GATGATGATG ACGATGATGA TGACCCTGCC ATGAAGCCCC AGTCTGTGCT CAACAGGGTC 
AAAATGTTCG AAAACAAACG CTCTGTGTCG GTAGACCGAG CCAAGGACGC ACCTGACGTC 
ACTGGAATTA GGCCCACAGA TCTCCCCAAA CCTGCGAGCA CCCCTGGACC TGTGCTCAAA 
GCCAACTCTC TCAGCAACCT GGAGCAGGAC AAACCCTCCT ACAGAGCTCC AGAGCCGCAG 
AAACCTCAGA CGCGTGTTGG GGACGATGTG GTTCGCTCCA ACCACTACGA CCCTGATGAT 
GATGAAGAGT ACTACAGAAA ACAGCTCTCA TTTTTCGACC GCCGCAGTTT CGACAGTAAG 
GTCATGAATC CACCCAGCGC TGGAGTAAAC CGCTTCCATG AGCCGCCCAA ACCACCCCAG 
ACACAGATCG CTTATCCTTT CGCTAGGACG GAGTCTGTGG AGAAGGTGAG TCCTGTGGAC 
AAGAGATACG AGCCTCTGCC GCCAGTCAAC CCTTCACCTG CTCCATACAG CCAGAACACA 
CCTGTCGCTC CGCCCACATC CCTGCCCAAA CTCACCAACA TCGAGGTGAA CTCTCTGCCA 
GATCCTCACA GCTCTCCCAA AGCCAAACCG GACCTGTCTG CTCTGAGAGC TCCGACCCGG 
GACGAGCCCA TCCAGAGCAG CTACTTGCCC CCCAAGTCCA CCATCAATGG CATGGATGCT 
CCTCCCAAAA CACTCGGCGT GCCGACCAGC TACAACCGCT ACGTCCCCAA GCCTTACACC 
AGCTCGGCGC GACCCTTCGA GCGCAAGTTC GAGAGTCCGA AATTCAACCA CAACCTGCTT 
CCCAACGACA CGCAGTCCAA ACCCAGTGTC AACAACAACC TGAAGCCTCA GATCTCCCCA 
CAACCCCAGG ACACGGACAG CGGCGTCGAC ACCTTCACTC GCACCATGGA CAACAGGCCA 
AAATATCAGC ACAACAACAT CAACGCCATT CCCAAAGCCG TCCCAGTCAG CCCCAGTGCA 
CTGGATGATG ATGAAGAAGA TGAGGGACAC ACAGTTGTGG CCACGGCGCG GGGCATCTTT 
AACTGTAATG GCGGGGTGTT GAGCTCTATA GAGACGGGCG TCAGCATCAT TATCCCACAG 
GGAGCCATTC CTGATAGTGT GGAGCAGGAG ATCTACTTCA AAGTGTGCCG GGACAACAGC 
ATCCTGCCTC CTCTGGATAA GGAGAAAGGT GAAACTCTGC TGAGTCCGCT GGTGATGTGT 
GGTCCTCACG GCCTGAAGTT CCTGAAGCCT GTGGAGCTGC GCTTGCCACA CTGTGCGTCT 
ATGACCCCTG ATGGTTGGTC TTTTGCTCTA AAATCCTCCG ACTCCTCGTC GGGTGACCCC 
AAATGCTGGC AAAACAAGTG TCTACCAGGA GACCCCAATT ACCTGGTGGG CGCAAACTGT 
GTGTCTGTGC TGATCGACCA CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326412.1
CDS	183..5549
Translation	MDETVIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGLLQENDRVVMVNAVSMDNVDHAYAVQQLRKSGKNAKITIRRKRKVQIPSGRPGERETMSEHDEDDDSYEDEIYESRSARSGPSAYSGGGGGGGGGGASGRRSGRGDRLSGRRERDRERSGSRERSLSPRSDRHSVSSNLPPRPAKVTLVKSRKNEACMASGSSQKVTSAEYGLRLASHIFVKDISPESLAARDGNIQEGDVVLKINGTVTENLSLIDAKKLIERSKGKLKMVVQRDERATLLNIPDLDDSIPSANASDRDDISDIHSVASDHSNRSHDRKRSSRSRSPDRRSEPSDHSRHSPPQISNGRATGPTAQRTRPVHRSRDEDRISKAGPLPLPAKMVEEMPKEQSAARETEKQLPPLPEPKPVYAQPGQPDVDLPVSPADAPVPSVTHDDSILRPSMKLVKFKKGESVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFANIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHQEVERGIIPNKNRAEQLSSVQYTLPKTPGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVVIFGPIADVAREKLSREETDLFELAKSEPRDAGTDQRSSGIIRLHTIKQIIDRDKHAVLDITPNAVDRLNYAQWYPIVVFLNPDSKQGVKNMRTRLCPESRKSARKLYERALKLRKNNHHLFTTTINLNNMNDGWYGALKESIQQQQNQLVWVSEGKADGAPDDDLDIHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETMNDEVGLPSEPAITRSSEPVREDPPVIQDAAGYPSYQHAVPQPEAVSRIDPAGFKMAAPQQQAEAALASPPPPSAAVQPTATLAGTNPEEPPGTPQADSLNSPVPVPDPQPEPELAQPPTHDPHQSLPPGPDPKMYKKDLYSMDEPVRVTHGVKPPPPQQQQQPLGPPTSLSYSHQPVYQDQQPYRQYDHPPYGYDGGGYAQPKPHNYDPHLHYDNRVPHYNEQWPTYDQQQQQQTSPQPPPASGFPQGHQPPPPPPMGYEPRSPYEDGPNRDFSPPQSQYDGVSPMGYDHRPRHPKPAPVRYEEPPPPPPPVSYDARSPFEPEPHAFPGNTHRSPDPPKQYYGDAAMRPLYNPGPPNRVYKHEPVNSEPPVPPPKPESVLSPGEPPHSAASKPLPPPPRDDDDDDDDDDDPAMKPQSVLNRVKMFENKRSVSVDRAKDAPDVTGIRPTDLPKPASTPGPVLKANSLSNLEQDKPSYRAPEPQKPQTRVGDDVVRSNHYDPDDDEEYYRKQLSFFDRRSFDSKVMNPPSAGVNRFHEPPKPPQTQIAYPFARTESVEKVSPVDKRYEPLPPVNPSPAPYSQNTPVAPPTSLPKLTNIEVNSLPDPHSSPKAKPDLSALRAPTRDEPIQSSYLPPKSTINGMDAPPKTLGVPTSYNRYVPKPYTSSARPFERKFESPKFNHNLLPNDTQSKPSVNNNLKPQISPQPQDTDSGVDTFTRTMDNRPKYQHNNINAIPKAVPVSPSALDDDEEDEGHTVVATARGIFNCNGGVLSSIETGVSIIIPQGAIPDSVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKCWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_021470737.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tight junction protein 1b (tjp1b), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470737.1

ORF Insert Sequence:

ATGGATGAGA CGGTAATATG GGAACAGCAC ACTGTAACTC TACACAGGGC TCCAGGGTTT 
GGCTTCGGGA TCGCCATCTC CGGCGGACGG GACAACCCTC ACTTCCAGAG CGGGGAGACG 
TCCATCGTCA TATCAGATGT GCTGAAGGGA GGCCCCGCCG AGGGCCTGCT GCAGGAGAAT 
GACAGAGTGG TGATGGTCAA CGCCGTCTCC ATGGACAATG TGGATCACGC TTACGCAGTG 
CAGCAGCTCC GCAAAAGTGG CAAGAATGCA AAAATAACCA TCAGGAGAAA GCGTAAGGTG 
CAGATCCCGT CAGGCCGGCC GGGTGAACGC GAGACTATGT CAGAGCATGA CGAGGACGAC 
GACAGCTACG AGGATGAGAT CTACGAGTCC CGGAGCGCGC GAAGCGGACC CAGCGCCTAC 
AGTGGAGGTG GAGGCGGCGG CGGAGGTGGA GGAGCCTCGG GACGGAGGAG CGGCAGAGGC 
GACAGGCTGA GTGGGAGGAG GGAGCGGGAC AGAGAGCGCA GCGGCTCCAG AGAGAGGAGT 
CTGTCCCCGC GCTCGGACCG CCACTCTGTG TCCTCCAACC TCCCCCCACG GCCAGCCAAA 
GTCACACTCG TCAAATCCCG CAAGAATGAA GCATGTATGG CGTCAGGAAG TTCTCAGAAG 
GTGACATCAG CAGAATACGG GCTTCGCCTG GCCAGCCACA TCTTCGTGAA GGACATTTCC 
CCCGAGAGCC TGGCAGCCCG AGACGGAAAC ATCCAAGAGG GAGACGTGGT GCTTAAGATT 
AATGGCACGG TAACAGAGAA TTTGTCCCTG ATCGATGCTA AGAAGCTGAT CGAGAGGTCA 
AAGGGAAAGC TGAAGATGGT GGTGCAGAGA GACGAGCGGG CGACGCTGCT GAACATCCCC 
GATCTGGACG ACAGCATACC TTCTGCTAAT GCCTCGGACA GAGATGATAT TTCAGACATC 
CACTCCGTGG CCTCTGATCA TTCCAACCGA TCCCACGACC GGAAGAGGAG CAGCCGCTCT 
CGTTCTCCAG ACCGCCGCTC CGAACCCTCA GACCACTCCA GACACTCTCC ACCACAGATC 
AGCAACGGCA GGGCCACAGG GCCGACGGCT CAGAGAACGC GGCCAGTTCA CAGGAGCCGT 
GATGAAGACA GGATCTCCAA GGCCGGGCCG CTTCCTCTAC CTGCTAAGAT GGTGGAGGAG 
ATGCCGAAAG AGCAGAGCGC AGCCAGAGAA ACAGAGAAAC AGCTCCCCCC TCTGCCAGAG 
CCCAAACCTG TGTACGCTCA GCCGGGCCAG CCGGACGTGG ACCTGCCTGT TAGTCCGGCA 
GATGCTCCTG TGCCCAGCGT CACACATGAT GACAGCATCT TACGGCCGAG CATGAAGCTG 
GTGAAGTTCA AGAAGGGCGA GAGCGTGGGC TTGAGGCTCG CTGGGGGGAA CGACGTTGGG 
ATATTTGTGG CGGGAGTTCT GGAGGACAGT CCTGCTGCTA AAGAGGGCCT GGAAGAGGGA 
GACCAGATTC TCAGGGTAAA TAATGTGGAT TTTGCAAATA TCATCCGAGA GGAGGCAGTG 
CTGTTCCTGC TGGATCTGCC CAAAGGAGAG GAGGTCACCA TCCTGGCCCA GAAGAAGAAA 
GACGTGTACC GCCGCATTGT AGAGTCTGAC GTGGGAGACT CGTTCTACAT CAGGACTCAT 
TTTGAATATG AGAAGGAGTC GCCCTATGGC CTGAGCTTCA ATAAGGGGGA GGTCTTCAGA 
GTCGTCGACA CCCTTTACAA TGGCAAACTG GGCTCCTGGC TCGCCATCCG CATCGGCAAA 
AACCACCAGG AGGTGGAGCG GGGCATCATC CCCAACAAGA ACAGGGCTGA GCAGTTATCA 
AGTGTCCAGT ACACTCTCCC AAAGACTCCA GGAGGGGATC GCGCCGACTT CTGGAGATTC 
AGAGGCCTCC GCAGCTCCAA ACGAAACCTG AGGAAGAGCA GAGAAGACCT GTCTGCTCAG 
CCGGTCCAAA CCAAATTCCC TGCTTATGAA AGAGTGGTGC TTAGAGAAGC TGGTTTCTTG 
AGGCCAGTGG TCATTTTCGG ACCGATCGCG GATGTGGCTA GAGAGAAGCT GTCCAGAGAG 
GAGACTGACC TCTTCGAACT TGCAAAGAGT GAACCCAGAG ATGCGGGCAC AGACCAGCGC 
AGCTCCGGAA TCATCCGTCT CCATACCATT AAACAGATCA TTGACCGAGA CAAGCACGCG 
GTGCTGGACA TCACCCCTAA TGCGGTGGAC CGTCTGAACT ACGCTCAGTG GTACCCCATC 
GTGGTGTTTC TGAATCCAGA CAGCAAACAG GGCGTCAAGA ACATGAGGAC CAGGCTCTGT 
CCCGAGTCCA GGAAGAGCGC CCGAAAGCTC TACGAGCGTG CTCTCAAACT GAGGAAGAAC 
AACCATCACC TTTTCACCAC CACTATTAAC TTGAACAACA TGAATGACGG GTGGTACGGT 
GCTTTGAAAG AGAGCATCCA GCAGCAGCAG AACCAGTTGG TCTGGGTCTC AGAGGGCAAG 
GCCGACGGCG CTCCTGACGA TGACCTTGAC ATCCATGACG ACCGCCTCTC CTACCTCTCG 
GCTCCGGGCA GCGAGTACAG CATGTACAGC ACCGACAGCC GCCACACGTC CGACTATGAG 
GACACCGACA CGGAGGGTGG TGCCTACACC GACCAGGAGC TGGACGAGAC CATGAACGAT 
GAGGTGGGCC TGCCTAGCGA GCCTGCCATC ACACGCTCAT CTGAGCCTGT GCGAGAGGAC 
CCGCCAGTCA TTCAGGACGC AGCTGGATAC CCCAGCTACC AGCATGCGGT ACCGCAACCT 
GAAGCGGTGA GCCGCATTGA CCCGGCCGGG TTTAAGATGG CAGCGCCGCA GCAGCAAGCT 
GAGGCTGCTC TGGCCTCGCC GCCCCCTCCC TCTGCAGCGG TCCAGCCCAC CGCAACACTA 
GCGGGTACGA ACCCCGAGGA GCCGCCTGGC ACCCCTCAGG CCGACTCCCT TAACAGCCCC 
GTCCCTGTTC CCGACCCCCA GCCCGAACCT GAGCTCGCTC AGCCCCCAAC ACACGACCCC 
CACCAGTCGC TTCCTCCTGG CCCAGATCCA AAGATGTATA AAAAGGATCT GTACAGCATG 
GACGAGCCGG TGCGAGTGAC CCACGGCGTG AAGCCTCCCC CTCCCCAGCA GCAGCAGCAG 
CCTTTAGGCC CCCCAACCTC GCTCTCCTAC AGCCACCAGC CTGTCTACCA GGACCAACAG 
CCATACCGCC AGTACGACCA CCCGCCTTAT GGCTATGACG GTGGCGGCTA CGCACAACCA 
AAGCCTCACA ACTACGACCC GCACCTGCAC TACGACAACC GTGTGCCTCA CTATAACGAG 
CAGTGGCCCA CGTACGACCA GCAGCAGCAG CAGCAGACTT CCCCCCAGCC GCCACCTGCG 
TCCGGCTTCC CTCAGGGCCA CCAGCCTCCG CCTCCACCAC CGATGGGATA TGAGCCACGC 
TCCCCATACG AGGACGGACC TAACAGGGAT TTCAGCCCTC CTCAGTCCCA GTATGACGGT 
GTGTCCCCAA TGGGCTACGA TCACCGGCCG CGGCACCCCA AACCTGCACC GGTCCGATAT 
GAAGAGCCTC CACCTCCACC TCCTCCGGTG TCCTACGATG CACGGTCACC TTTCGAACCC 
GAGCCGCATG CTTTCCCAGG CAACACACAT CGCTCGCCAG ACCCACCGAA ACAGTATTAT 
GGAGACGCCG CTATGAGGCC CTTGTACAAC CCTGGACCTC CGAACAGAGT GTACAAACAT 
GAGCCGGTGA ACTCTGAGCC TCCTGTACCG CCTCCCAAAC CTGAATCTGT CCTGTCTCCA 
GGAGAACCGC CCCATTCTGC AGCTTCCAAA CCATTGCCGC CTCCTCCCAG AGATGATGAC 
GATGATGATG ACGATGATGA TGACCCTGCC ATGAAGCCCC AGTCTGTGCT CAACAGGGTC 
AAAATGTTCG AAAACAAACG CTCTGTGTCG GTAGACCGAG CCAAGGACGC ACCTGACGTC 
ACTGGAATTA GGCCCACAGA TCTCCCCAAA CCTGCGAGCA CCCCTGGACC TGTGCTCAAA 
GCCAACTCTC TCAGCAACCT GGAGCAGGAC AAACCCTCCT ACAGAGCTCC AGAGCCGCAG 
AAACCTCAGA CGCGTGTTGG GGACGATGTG GTTCGCTCCA ACCACTACGA CCCTGATGAT 
GATGAAGAGT ACTACAGAAA ACAGCTCTCA TTTTTCGACC GCCGCAGTTT CGACAGTAAG 
GTCATGAATC CACCCAGCGC TGGAGTAAAC CGCTTCCATG AGCCGCCCAA ACCACCCCAG 
ACACAGATCG CTTATCCTTT CGCTAGGACG GAGTCTGTGG AGAAGGTGAG TCCTGTGGAC 
AAGAGATACG AGCCTCTGCC GCCAGTCAAC CCTTCACCTG CTCCATACAG CCAGAACACA 
CCTGTCGCTC CGCCCACATC CCTGCCCAAA CTCACCAACA TCGAGGTGAA CTCTCTGCCA 
GATCCTCACA GCTCTCCCAA AGCCAAACCG GACCTGTCTG CTCTGAGAGC TCCGACCCGG 
GACGAGCCCA TCCAGAGCAG CTACTTGCCC CCCAAGTCCA CCATCAATGG CATGGATGCT 
CCTCCCAAAA CACTCGGCGT GCCGACCAGC TACAACCGCT ACGTCCCCAA GCCTTACACC 
AGCTCGGCGC GACCCTTCGA GCGCAAGTTC GAGAGTCCGA AATTCAACCA CAACCTGCTT 
CCCAACGACA CGCAGTCCAA ACCCAGTGTC AACAACAACC TGAAGCCTCA GATCTCCCCA 
CAACCCCAGG ACACGGACAG CGGCGTCGAC ACCTTCACTC GCACCATGGA CAACAGGCCA 
AAATATCAGC ACAACAACAT CAACGCCATT CCCAAAGCCG TCCCAGTCAG CCCCAGTGCA 
CTGGATGATG ATGAAGAAGA TGAGGGACAC ACAGTTGTGG CCACGGCGCG GGGCATCTTT 
AACTGTAATG GCGGGGTGTT GAGCTCTATA GAGACGGGCG TCAGCATCAT TATCCCACAG 
GGAGCCATTC CTGATAGTGT GGAGCAGGAG ATCTACTTCA AAGTGTGCCG GGACAACAGC 
ATCCTGCCTC CTCTGGATAA GGAGAAAGGT GAAACTCTGC TGAGTCCGCT GGTGATGTGT 
GGTCCTCACG GCCTGAAGTT CCTGAAGCCT GTGGAGCTGC GCTTGCCACA CTGTGCGTCT 
ATGACCCCTG ATGGTTGGTC TTTTGCTCTA AAATCCTCCG ACTCCTCGTC GGGTGACCCC 
AAATGCTGGC AAAACAAGTG TCTACCAGGA GACCCCAATT ACCTGGTGGG CGCAAACTGT 
GTGTCTGTGC TGATCGACCA CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp1b ( XM_009298141.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296416.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X15, mRNA.

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CloneID	ODa44631
Clone ID Related Accession (Same CDS sequence)	XM_009298141.2 , XM_009298141.3
Accession Version	XM_009298141.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5994bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tight junction protein ZO-1 isoform X15
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298141.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
TCCCGCAAGA ATGAAGAATA CGGGCTTCGC CTGGCCAGCC ACATCTTCGT GAAGGACATT 
TCCCCCGAGA GCCTGGCAGC CCGAGACGGA AACATCCAAG AGGGAGACGT GGTGCTTAAG 
ATTAATGGCA CGGTAACAGA GAATTTGTCC CTGATCGATG CTAAGAAGCT GATCGAGAGG 
TCAAAGGGAA AGCTGAAGAT GGTGGTGCAG AGAGACGAGC GGGCGACGCT GCTGAACATC 
CCCGATCTGG ACGACAGCAT ACCTTCTGCT AATGCCTCGG ACAGAGATGA TATTTCAGAC 
ATCCACTCCG TGGCCTCTGA TCATTCCAAC CGATCCCACG ACCGGAAGAG GAGCAGCCGC 
TCTCGTTCTC CAGACCGCCG CTCCGAACCC TCAGACCACT CCAGACACTC TCCACCACAG 
ATCAGCAACG GCAGTCACAG GAGCCGTGAT GAAGACAGGA TCTCCAAGGC CGGGCCGCTT 
CCTCTACCTG CTAAGATGGT GGAGGAGATG CCGAAAGAGC AGAGCGCAGC CAGAGAAACA 
GAGAAACAGC TCCCCCCTCT GCCAGAGCCC AAACCTGTGT ACGCTCAGCC GGGCCAGCCG 
GACGTGGACC TGCCTGTTAG TCCGGCAGAT GCTCCTGTGC CCAGCGTCAC ACATGATGAC 
AGCATCTTAC GGCCGAGCAT GAAGCTGGTG AAGTTCAAGA AGGGCGAGAG CGTGGGCTTG 
AGGCTCGCTG GGGGGAACGA CGTTGGGATA TTTGTGGCGG GAGTTCTGGA GGACAGTCCT 
GCTGCTAAAG AGGGCCTGGA AGAGGGAGAC CAGATTCTCA GGGTAAATAA TGTGGATTTT 
GCAAATATCA TCCGAGAGGA GGCAGTGCTG TTCCTGCTGG ATCTGCCCAA AGGAGAGGAG 
GTCACCATCC TGGCCCAGAA GAAGAAAGAC GTGTACCGCC GCATTGTAGA GTCTGACGTG 
GGAGACTCGT TCTACATCAG GACTCATTTT GAATATGAGA AGGAGTCGCC CTATGGCCTG 
AGCTTCAATA AGGGGGAGGT CTTCAGAGTC GTCGACACCC TTTACAATGG CAAACTGGGC 
TCCTGGCTCG CCATCCGCAT CGGCAAAAAC CACCAGGAGG TGGAGCGGGG CATCATCCCC 
AACAAGAACA GGGCTGAGCA GTTATCAAGT GTCCAGTACA CTCTCCCAAA GACTCCAGGA 
GGGGATCGCG CCGACTTCTG GAGATTCAGA GGCCTCCGCA GCTCCAAACG AAACCTGAGG 
AAGAGCAGAG AAGACCTGTC TGCTCAGCCG GTCCAAACCA AATTCCCTGC TTATGAAAGA 
GTGGTGCTTA GAGAAGCTGG TTTCTTGAGG CCAGTGGTCA TTTTCGGACC GATCGCGGAT 
GTGGCTAGAG AGAAGCTGTC CAGAGAGGAG ACTGACCTCT TCGAACTTGC AAAGAGTGAA 
CCCAGAGATG CGGGCACAGA CCAGCGCAGC TCCGGAATCA TCCGTCTCCA TACCATTAAA 
CAGATCATTG ACCGAGACAA GCACGCGGTG CTGGACATCA CCCCTAATGC GGTGGACCGT 
CTGAACTACG CTCAGTGGTA CCCCATCGTG GTGTTTCTGA ATCCAGACAG CAAACAGGGC 
GTCAAGAACA TGAGGACCAG GCTCTGTCCC GAGTCCAGGA AGAGCGCCCG AAAGCTCTAC 
GAGCGTGCTC TCAAACTGAG GAAGAACAAC CATCACCTTT TCACCACCAC TATTAACTTG 
AACAACATGA ATGACGGGTG GTACGGTGCT TTGAAAGAGA GCATCCAGCA GCAGCAGAAC 
CAGTTGGTCT GGGTCTCAGA GGGCAAGGCC GACGGCGCTC CTGACGATGA CCTTGACATC 
CATGACGACC GCCTCTCCTA CCTCTCGGCT CCGGGCAGCG AGTACAGCAT GTACAGCACC 
GACAGCCGCC ACACGTCCGA CTATGAGGAC ACCGACACGG AGGGTGGTGC CTACACCGAC 
CAGGAGCTGG ACGAGACCAT GAACGATGAG GTGGGCCTGC CTAGCGAGCC TGCCATCACA 
CGCTCATCTG AGCCTGTGCG AGAGGACCCG CCAGTCATTC AGGACGCAGC TGGATACCCC 
AGCTACCAGC ATGCGGTACC GCAACCTGAA GCGGTGAGCC GCATTGACCC GGCCGGGTTT 
AAGATGGCAG CGCCGCAGCA GCAAGCTGAG GCTGCTCTGG CCTCGCCGCC CCCTCCCTCT 
GCAGCGGTCC AGCCCACCGC AACACTAGCG GGTACGAACC CCGAGGAGCC GCCTGGCACC 
CCTCAGGCCG ACTCCCTTAA CAGCCCCGTC CCTGTTCCCG ACCCCCAGCC CGAACCTGAG 
CTCGCTCAGC CCCCAACACA CGACCCCCAC CAGTCGCTTC CTCCTGGCCC AGATCCAAAG 
ATGTATAAAA AGGATCTGTA CAGCATGGAC GAGCCGGTGC GAGTGACCCA CGGCGTGAAG 
CCTCCCCCTC CCCAGCAGCA GCAGCAGCCT TTAGGCCCCC CAACCTCGCT CTCCTACAGC 
CACCAGCCTG TCTACCAGGA CCAACAGCCA TACCGCCAGT ACGACCACCC GCCTTATGGC 
TATGACGGTG GCGGCTACGC ACAACCAAAG CCTCACAACT ACGACCCGCA CCTGCACTAC 
GACAACCGTG TGCCTCACTA TAACGAGCAG TGGCCCACGT ACGACCAGCA GCAGCAGCAG 
CAGACTTCCC CCCAGCCGCC ACCTGCGTCC GGCTTCCCTC AGGGCCACCA GCCTCCGCCT 
CCACCACCGA TGGGATATGA GCCACGCTCC CCATACGAGG ACGGACCTAA CAGGGATTTC 
AGCCCTCCTC AGTCCCAGTA TGACGGTGTG TCCCCAATGG GCTACGATCA CCGGCCGCGG 
CACCCCAAAC CTGCACCGGT CCGATATGAA GAGCCTCCAC CTCCACCTCC TCCGGTGTCC 
TACGATGCAC GGTCACCTTT CGAACCCGAG CCGCATGCTT TCCCAGGCAA CACACATCGC 
TCGCCAGACC CACCGAAACA GTATTATGGA GACGCCGCTA TGAGGCCCTT GTACAACCCT 
GGACCTCCGA ACAGAGTGTA CAAACATGAG CCGGTGAACT CTGAGCCTCC TGTACCGCCT 
CCCAAACCTG AATCTGTCCT GTCTCCAGGA GAACCGCCCC ATTCTGCAGC TTCCAAACCA 
TTGCCGCCTC CTCCCAGAGA TGATGACGAT GATGATGACG ATGATGATGA CCCTGCCATG 
AAGCCCCAGT CTGTGCTCAA CAGGGTCAAA ATGTTCGAAA ACAAACGCTC TGTGTCGGTA 
GACCGAGCCA AGGACGCACC TGACGTCACT GGAATTAGGC CCACAGATCT CCCCAAACCT 
GCGAGCACCC CTGGACCTGT GCTCAAAGCC AACTCTCTCA GCAACCTGGA GCAGGACAAA 
CCCTCCTACA GAGCTCCAGA GCCGCAGAAA CCTCAGACGC GTGTTGGGGA CGATGTGGTT 
CGCTCCAACC ACTACGACCC TGATGATGAT GAAGAGTACT ACAGAAAACA GCTCTCATTT 
TTCGACCGCC GCAGTTTCGA CAGTAAGGTC ATGAATCCAC CCAGCGCTGG AGTAAACCGC 
TTCCATGAGC CGCCCAAACC ACCCCAGACA CAGATCGCTT ATCCTTTCGC TAGGACGGAG 
TCTGTGGAGA AGGTGAGTCC TGTGGACAAG AGATACGAGC CTCTGCCGCC AGTCAACCCT 
TCACCTGCTC CATACAGCCA GAACACACCT GTCGCTCCGC CCACATCCCT GCCCAAACTC 
ACCAACATCG AGGTGAACTC TCTGCCAGAT CCTCACAGCT CTCCCAAAGC CAAACCGGAC 
CTGTCTGCTC TGAGAGCTCC GACCCGGGAC GAGCCCATCC AGAGCAGCTA CTTGCCCCCC 
AAGTCCACCA TCAATGGCAT GGATGCTCCT CCCAAAACAC TCGGCGTGCC GACCAGCTAC 
AACCGCTACG TCCCCAAGCC TTACACCAGC TCGGCGCGAC CCTTCGAGCG CAAGTTCGAG 
AGTCCGAAAT TCAACCACAA CCTGCTTCCC AACGACACGC AGTCCAAACC CAGTGTCAAC 
AACAACCTGA AGCCTCAGAT CTCCCCACAA CCCCAGGACA CGGACAGCGG CGTCGACACC 
TTCACTCGCA CCATGGACAA CAGGCCAAAA TATCAGCACA ACAACATCAA CGCCATTCCC 
AAAGCCGTCC CAGTCAGCCC CAGTGCACTG GATGATGATG AAGAAGATGA GGGACACACA 
GTTGTGGCCA CGGCGCGGGG CATCTTTAAC TGTAATGGCG GGGTGTTGAG CTCTATAGAG 
ACGGGCGTCA GCATCATTAT CCCACAGGGA GCCATTCCTG ATAGTGTGGA GCAGGAGATC 
TACTTCAAAG TGTGCCGGGA CAACAGCATC CTGCCTCCTC TGGATAAGGA GAAAGGTGAA 
ACTCTGCTGA GTCCGCTGGT GATGTGTGGT CCTCACGGCC TGAAGTTCCT GAAGCCTGTG 
GAGCTGCGCT TGCCACACTG TGACCCCAAA TGCTGGCAAA ACAAGTGTCT ACCAGGAGAC 
CCCAATTACC TGGTGGGCGC AAACTGTGTG TCTGTGCTGA TCGACCACTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296416.1
CDS	115..6108
Translation	MLLSVFGGPVEEVLAGLLKLKMFGSGSKKWFIKKNKRQRTKVQRKPASGRRNKRVSRVLKRHKEVIVKQLDVSKVLPTLVYERVFTLGEYKEILGQDSSGKRAELFLDLLSQKEPAAFSAFCGVLEEVCPHLLTCFLLDGTDGSAGENKKQGQTKPAHSQQNALSKGQDTVCFPPMYTDPLFDTRVFPKHDKDKYGPAVYYPKFNAYIPNPDKVSVLDESEGSGHGKPSLRRIKGRIHRSKSLDSIDLLDSNSAAMDETVIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGLLQENDRVVMVNAVSMDNVDHAYAVQQLRKSGKNAKITIRRKRKVQIPSGRPGERETMSEHDEDDDSYEDEIYESRSARSGPSAYSGGGGGGGGGGASGRRSGRGDRLSGRRERDRERSGSRERSLSPRSDRHSVSSNLPPRPAKVTLVKSRKNEEYGLRLASHIFVKDISPESLAARDGNIQEGDVVLKINGTVTENLSLIDAKKLIERSKGKLKMVVQRDERATLLNIPDLDDSIPSANASDRDDISDIHSVASDHSNRSHDRKRSSRSRSPDRRSEPSDHSRHSPPQISNGSHRSRDEDRISKAGPLPLPAKMVEEMPKEQSAARETEKQLPPLPEPKPVYAQPGQPDVDLPVSPADAPVPSVTHDDSILRPSMKLVKFKKGESVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFANIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHQEVERGIIPNKNRAEQLSSVQYTLPKTPGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVVIFGPIADVAREKLSREETDLFELAKSEPRDAGTDQRSSGIIRLHTIKQIIDRDKHAVLDITPNAVDRLNYAQWYPIVVFLNPDSKQGVKNMRTRLCPESRKSARKLYERALKLRKNNHHLFTTTINLNNMNDGWYGALKESIQQQQNQLVWVSEGKADGAPDDDLDIHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETMNDEVGLPSEPAITRSSEPVREDPPVIQDAAGYPSYQHAVPQPEAVSRIDPAGFKMAAPQQQAEAALASPPPPSAAVQPTATLAGTNPEEPPGTPQADSLNSPVPVPDPQPEPELAQPPTHDPHQSLPPGPDPKMYKKDLYSMDEPVRVTHGVKPPPPQQQQQPLGPPTSLSYSHQPVYQDQQPYRQYDHPPYGYDGGGYAQPKPHNYDPHLHYDNRVPHYNEQWPTYDQQQQQQTSPQPPPASGFPQGHQPPPPPPMGYEPRSPYEDGPNRDFSPPQSQYDGVSPMGYDHRPRHPKPAPVRYEEPPPPPPPVSYDARSPFEPEPHAFPGNTHRSPDPPKQYYGDAAMRPLYNPGPPNRVYKHEPVNSEPPVPPPKPESVLSPGEPPHSAASKPLPPPPRDDDDDDDDDDDPAMKPQSVLNRVKMFENKRSVSVDRAKDAPDVTGIRPTDLPKPASTPGPVLKANSLSNLEQDKPSYRAPEPQKPQTRVGDDVVRSNHYDPDDDEEYYRKQLSFFDRRSFDSKVMNPPSAGVNRFHEPPKPPQTQIAYPFARTESVEKVSPVDKRYEPLPPVNPSPAPYSQNTPVAPPTSLPKLTNIEVNSLPDPHSSPKAKPDLSALRAPTRDEPIQSSYLPPKSTINGMDAPPKTLGVPTSYNRYVPKPYTSSARPFERKFESPKFNHNLLPNDTQSKPSVNNNLKPQISPQPQDTDSGVDTFTRTMDNRPKYQHNNINAIPKAVPVSPSALDDDEEDEGHTVVATARGIFNCNGGVLSSIETGVSIIIPQGAIPDSVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCDPKCWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_009298141.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tight junction protein 1b (tjp1b), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298141.2

ORF Insert Sequence:

ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
TCCCGCAAGA ATGAAGAATA CGGGCTTCGC CTGGCCAGCC ACATCTTCGT GAAGGACATT 
TCCCCCGAGA GCCTGGCAGC CCGAGACGGA AACATCCAAG AGGGAGACGT GGTGCTTAAG 
ATTAATGGCA CGGTAACAGA GAATTTGTCC CTGATCGATG CTAAGAAGCT GATCGAGAGG 
TCAAAGGGAA AGCTGAAGAT GGTGGTGCAG AGAGACGAGC GGGCGACGCT GCTGAACATC 
CCCGATCTGG ACGACAGCAT ACCTTCTGCT AATGCCTCGG ACAGAGATGA TATTTCAGAC 
ATCCACTCCG TGGCCTCTGA TCATTCCAAC CGATCCCACG ACCGGAAGAG GAGCAGCCGC 
TCTCGTTCTC CAGACCGCCG CTCCGAACCC TCAGACCACT CCAGACACTC TCCACCACAG 
ATCAGCAACG GCAGTCACAG GAGCCGTGAT GAAGACAGGA TCTCCAAGGC CGGGCCGCTT 
CCTCTACCTG CTAAGATGGT GGAGGAGATG CCGAAAGAGC AGAGCGCAGC CAGAGAAACA 
GAGAAACAGC TCCCCCCTCT GCCAGAGCCC AAACCTGTGT ACGCTCAGCC GGGCCAGCCG 
GACGTGGACC TGCCTGTTAG TCCGGCAGAT GCTCCTGTGC CCAGCGTCAC ACATGATGAC 
AGCATCTTAC GGCCGAGCAT GAAGCTGGTG AAGTTCAAGA AGGGCGAGAG CGTGGGCTTG 
AGGCTCGCTG GGGGGAACGA CGTTGGGATA TTTGTGGCGG GAGTTCTGGA GGACAGTCCT 
GCTGCTAAAG AGGGCCTGGA AGAGGGAGAC CAGATTCTCA GGGTAAATAA TGTGGATTTT 
GCAAATATCA TCCGAGAGGA GGCAGTGCTG TTCCTGCTGG ATCTGCCCAA AGGAGAGGAG 
GTCACCATCC TGGCCCAGAA GAAGAAAGAC GTGTACCGCC GCATTGTAGA GTCTGACGTG 
GGAGACTCGT TCTACATCAG GACTCATTTT GAATATGAGA AGGAGTCGCC CTATGGCCTG 
AGCTTCAATA AGGGGGAGGT CTTCAGAGTC GTCGACACCC TTTACAATGG CAAACTGGGC 
TCCTGGCTCG CCATCCGCAT CGGCAAAAAC CACCAGGAGG TGGAGCGGGG CATCATCCCC 
AACAAGAACA GGGCTGAGCA GTTATCAAGT GTCCAGTACA CTCTCCCAAA GACTCCAGGA 
GGGGATCGCG CCGACTTCTG GAGATTCAGA GGCCTCCGCA GCTCCAAACG AAACCTGAGG 
AAGAGCAGAG AAGACCTGTC TGCTCAGCCG GTCCAAACCA AATTCCCTGC TTATGAAAGA 
GTGGTGCTTA GAGAAGCTGG TTTCTTGAGG CCAGTGGTCA TTTTCGGACC GATCGCGGAT 
GTGGCTAGAG AGAAGCTGTC CAGAGAGGAG ACTGACCTCT TCGAACTTGC AAAGAGTGAA 
CCCAGAGATG CGGGCACAGA CCAGCGCAGC TCCGGAATCA TCCGTCTCCA TACCATTAAA 
CAGATCATTG ACCGAGACAA GCACGCGGTG CTGGACATCA CCCCTAATGC GGTGGACCGT 
CTGAACTACG CTCAGTGGTA CCCCATCGTG GTGTTTCTGA ATCCAGACAG CAAACAGGGC 
GTCAAGAACA TGAGGACCAG GCTCTGTCCC GAGTCCAGGA AGAGCGCCCG AAAGCTCTAC 
GAGCGTGCTC TCAAACTGAG GAAGAACAAC CATCACCTTT TCACCACCAC TATTAACTTG 
AACAACATGA ATGACGGGTG GTACGGTGCT TTGAAAGAGA GCATCCAGCA GCAGCAGAAC 
CAGTTGGTCT GGGTCTCAGA GGGCAAGGCC GACGGCGCTC CTGACGATGA CCTTGACATC 
CATGACGACC GCCTCTCCTA CCTCTCGGCT CCGGGCAGCG AGTACAGCAT GTACAGCACC 
GACAGCCGCC ACACGTCCGA CTATGAGGAC ACCGACACGG AGGGTGGTGC CTACACCGAC 
CAGGAGCTGG ACGAGACCAT GAACGATGAG GTGGGCCTGC CTAGCGAGCC TGCCATCACA 
CGCTCATCTG AGCCTGTGCG AGAGGACCCG CCAGTCATTC AGGACGCAGC TGGATACCCC 
AGCTACCAGC ATGCGGTACC GCAACCTGAA GCGGTGAGCC GCATTGACCC GGCCGGGTTT 
AAGATGGCAG CGCCGCAGCA GCAAGCTGAG GCTGCTCTGG CCTCGCCGCC CCCTCCCTCT 
GCAGCGGTCC AGCCCACCGC AACACTAGCG GGTACGAACC CCGAGGAGCC GCCTGGCACC 
CCTCAGGCCG ACTCCCTTAA CAGCCCCGTC CCTGTTCCCG ACCCCCAGCC CGAACCTGAG 
CTCGCTCAGC CCCCAACACA CGACCCCCAC CAGTCGCTTC CTCCTGGCCC AGATCCAAAG 
ATGTATAAAA AGGATCTGTA CAGCATGGAC GAGCCGGTGC GAGTGACCCA CGGCGTGAAG 
CCTCCCCCTC CCCAGCAGCA GCAGCAGCCT TTAGGCCCCC CAACCTCGCT CTCCTACAGC 
CACCAGCCTG TCTACCAGGA CCAACAGCCA TACCGCCAGT ACGACCACCC GCCTTATGGC 
TATGACGGTG GCGGCTACGC ACAACCAAAG CCTCACAACT ACGACCCGCA CCTGCACTAC 
GACAACCGTG TGCCTCACTA TAACGAGCAG TGGCCCACGT ACGACCAGCA GCAGCAGCAG 
CAGACTTCCC CCCAGCCGCC ACCTGCGTCC GGCTTCCCTC AGGGCCACCA GCCTCCGCCT 
CCACCACCGA TGGGATATGA GCCACGCTCC CCATACGAGG ACGGACCTAA CAGGGATTTC 
AGCCCTCCTC AGTCCCAGTA TGACGGTGTG TCCCCAATGG GCTACGATCA CCGGCCGCGG 
CACCCCAAAC CTGCACCGGT CCGATATGAA GAGCCTCCAC CTCCACCTCC TCCGGTGTCC 
TACGATGCAC GGTCACCTTT CGAACCCGAG CCGCATGCTT TCCCAGGCAA CACACATCGC 
TCGCCAGACC CACCGAAACA GTATTATGGA GACGCCGCTA TGAGGCCCTT GTACAACCCT 
GGACCTCCGA ACAGAGTGTA CAAACATGAG CCGGTGAACT CTGAGCCTCC TGTACCGCCT 
CCCAAACCTG AATCTGTCCT GTCTCCAGGA GAACCGCCCC ATTCTGCAGC TTCCAAACCA 
TTGCCGCCTC CTCCCAGAGA TGATGACGAT GATGATGACG ATGATGATGA CCCTGCCATG 
AAGCCCCAGT CTGTGCTCAA CAGGGTCAAA ATGTTCGAAA ACAAACGCTC TGTGTCGGTA 
GACCGAGCCA AGGACGCACC TGACGTCACT GGAATTAGGC CCACAGATCT CCCCAAACCT 
GCGAGCACCC CTGGACCTGT GCTCAAAGCC AACTCTCTCA GCAACCTGGA GCAGGACAAA 
CCCTCCTACA GAGCTCCAGA GCCGCAGAAA CCTCAGACGC GTGTTGGGGA CGATGTGGTT 
CGCTCCAACC ACTACGACCC TGATGATGAT GAAGAGTACT ACAGAAAACA GCTCTCATTT 
TTCGACCGCC GCAGTTTCGA CAGTAAGGTC ATGAATCCAC CCAGCGCTGG AGTAAACCGC 
TTCCATGAGC CGCCCAAACC ACCCCAGACA CAGATCGCTT ATCCTTTCGC TAGGACGGAG 
TCTGTGGAGA AGGTGAGTCC TGTGGACAAG AGATACGAGC CTCTGCCGCC AGTCAACCCT 
TCACCTGCTC CATACAGCCA GAACACACCT GTCGCTCCGC CCACATCCCT GCCCAAACTC 
ACCAACATCG AGGTGAACTC TCTGCCAGAT CCTCACAGCT CTCCCAAAGC CAAACCGGAC 
CTGTCTGCTC TGAGAGCTCC GACCCGGGAC GAGCCCATCC AGAGCAGCTA CTTGCCCCCC 
AAGTCCACCA TCAATGGCAT GGATGCTCCT CCCAAAACAC TCGGCGTGCC GACCAGCTAC 
AACCGCTACG TCCCCAAGCC TTACACCAGC TCGGCGCGAC CCTTCGAGCG CAAGTTCGAG 
AGTCCGAAAT TCAACCACAA CCTGCTTCCC AACGACACGC AGTCCAAACC CAGTGTCAAC 
AACAACCTGA AGCCTCAGAT CTCCCCACAA CCCCAGGACA CGGACAGCGG CGTCGACACC 
TTCACTCGCA CCATGGACAA CAGGCCAAAA TATCAGCACA ACAACATCAA CGCCATTCCC 
AAAGCCGTCC CAGTCAGCCC CAGTGCACTG GATGATGATG AAGAAGATGA GGGACACACA 
GTTGTGGCCA CGGCGCGGGG CATCTTTAAC TGTAATGGCG GGGTGTTGAG CTCTATAGAG 
ACGGGCGTCA GCATCATTAT CCCACAGGGA GCCATTCCTG ATAGTGTGGA GCAGGAGATC 
TACTTCAAAG TGTGCCGGGA CAACAGCATC CTGCCTCCTC TGGATAAGGA GAAAGGTGAA 
ACTCTGCTGA GTCCGCTGGT GATGTGTGGT CCTCACGGCC TGAAGTTCCT GAAGCCTGTG 
GAGCTGCGCT TGCCACACTG TGACCCCAAA TGCTGGCAAA ACAAGTGTCT ACCAGGAGAC 
CCCAATTACC TGGTGGGCGC AAACTGTGTG TCTGTGCTGA TCGACCACTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp1b ( XM_009298141.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296416.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X20, mRNA.

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CloneID	ODa44631
Clone ID Related Accession (Same CDS sequence)	XM_009298141.2 , XM_009298141.3
Accession Version	XM_009298141.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5994bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tight junction protein ZO-1 isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009298141.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
TCCCGCAAGA ATGAAGAATA CGGGCTTCGC CTGGCCAGCC ACATCTTCGT GAAGGACATT 
TCCCCCGAGA GCCTGGCAGC CCGAGACGGA AACATCCAAG AGGGAGACGT GGTGCTTAAG 
ATTAATGGCA CGGTAACAGA GAATTTGTCC CTGATCGATG CTAAGAAGCT GATCGAGAGG 
TCAAAGGGAA AGCTGAAGAT GGTGGTGCAG AGAGACGAGC GGGCGACGCT GCTGAACATC 
CCCGATCTGG ACGACAGCAT ACCTTCTGCT AATGCCTCGG ACAGAGATGA TATTTCAGAC 
ATCCACTCCG TGGCCTCTGA TCATTCCAAC CGATCCCACG ACCGGAAGAG GAGCAGCCGC 
TCTCGTTCTC CAGACCGCCG CTCCGAACCC TCAGACCACT CCAGACACTC TCCACCACAG 
ATCAGCAACG GCAGTCACAG GAGCCGTGAT GAAGACAGGA TCTCCAAGGC CGGGCCGCTT 
CCTCTACCTG CTAAGATGGT GGAGGAGATG CCGAAAGAGC AGAGCGCAGC CAGAGAAACA 
GAGAAACAGC TCCCCCCTCT GCCAGAGCCC AAACCTGTGT ACGCTCAGCC GGGCCAGCCG 
GACGTGGACC TGCCTGTTAG TCCGGCAGAT GCTCCTGTGC CCAGCGTCAC ACATGATGAC 
AGCATCTTAC GGCCGAGCAT GAAGCTGGTG AAGTTCAAGA AGGGCGAGAG CGTGGGCTTG 
AGGCTCGCTG GGGGGAACGA CGTTGGGATA TTTGTGGCGG GAGTTCTGGA GGACAGTCCT 
GCTGCTAAAG AGGGCCTGGA AGAGGGAGAC CAGATTCTCA GGGTAAATAA TGTGGATTTT 
GCAAATATCA TCCGAGAGGA GGCAGTGCTG TTCCTGCTGG ATCTGCCCAA AGGAGAGGAG 
GTCACCATCC TGGCCCAGAA GAAGAAAGAC GTGTACCGCC GCATTGTAGA GTCTGACGTG 
GGAGACTCGT TCTACATCAG GACTCATTTT GAATATGAGA AGGAGTCGCC CTATGGCCTG 
AGCTTCAATA AGGGGGAGGT CTTCAGAGTC GTCGACACCC TTTACAATGG CAAACTGGGC 
TCCTGGCTCG CCATCCGCAT CGGCAAAAAC CACCAGGAGG TGGAGCGGGG CATCATCCCC 
AACAAGAACA GGGCTGAGCA GTTATCAAGT GTCCAGTACA CTCTCCCAAA GACTCCAGGA 
GGGGATCGCG CCGACTTCTG GAGATTCAGA GGCCTCCGCA GCTCCAAACG AAACCTGAGG 
AAGAGCAGAG AAGACCTGTC TGCTCAGCCG GTCCAAACCA AATTCCCTGC TTATGAAAGA 
GTGGTGCTTA GAGAAGCTGG TTTCTTGAGG CCAGTGGTCA TTTTCGGACC GATCGCGGAT 
GTGGCTAGAG AGAAGCTGTC CAGAGAGGAG ACTGACCTCT TCGAACTTGC AAAGAGTGAA 
CCCAGAGATG CGGGCACAGA CCAGCGCAGC TCCGGAATCA TCCGTCTCCA TACCATTAAA 
CAGATCATTG ACCGAGACAA GCACGCGGTG CTGGACATCA CCCCTAATGC GGTGGACCGT 
CTGAACTACG CTCAGTGGTA CCCCATCGTG GTGTTTCTGA ATCCAGACAG CAAACAGGGC 
GTCAAGAACA TGAGGACCAG GCTCTGTCCC GAGTCCAGGA AGAGCGCCCG AAAGCTCTAC 
GAGCGTGCTC TCAAACTGAG GAAGAACAAC CATCACCTTT TCACCACCAC TATTAACTTG 
AACAACATGA ATGACGGGTG GTACGGTGCT TTGAAAGAGA GCATCCAGCA GCAGCAGAAC 
CAGTTGGTCT GGGTCTCAGA GGGCAAGGCC GACGGCGCTC CTGACGATGA CCTTGACATC 
CATGACGACC GCCTCTCCTA CCTCTCGGCT CCGGGCAGCG AGTACAGCAT GTACAGCACC 
GACAGCCGCC ACACGTCCGA CTATGAGGAC ACCGACACGG AGGGTGGTGC CTACACCGAC 
CAGGAGCTGG ACGAGACCAT GAACGATGAG GTGGGCCTGC CTAGCGAGCC TGCCATCACA 
CGCTCATCTG AGCCTGTGCG AGAGGACCCG CCAGTCATTC AGGACGCAGC TGGATACCCC 
AGCTACCAGC ATGCGGTACC GCAACCTGAA GCGGTGAGCC GCATTGACCC GGCCGGGTTT 
AAGATGGCAG CGCCGCAGCA GCAAGCTGAG GCTGCTCTGG CCTCGCCGCC CCCTCCCTCT 
GCAGCGGTCC AGCCCACCGC AACACTAGCG GGTACGAACC CCGAGGAGCC GCCTGGCACC 
CCTCAGGCCG ACTCCCTTAA CAGCCCCGTC CCTGTTCCCG ACCCCCAGCC CGAACCTGAG 
CTCGCTCAGC CCCCAACACA CGACCCCCAC CAGTCGCTTC CTCCTGGCCC AGATCCAAAG 
ATGTATAAAA AGGATCTGTA CAGCATGGAC GAGCCGGTGC GAGTGACCCA CGGCGTGAAG 
CCTCCCCCTC CCCAGCAGCA GCAGCAGCCT TTAGGCCCCC CAACCTCGCT CTCCTACAGC 
CACCAGCCTG TCTACCAGGA CCAACAGCCA TACCGCCAGT ACGACCACCC GCCTTATGGC 
TATGACGGTG GCGGCTACGC ACAACCAAAG CCTCACAACT ACGACCCGCA CCTGCACTAC 
GACAACCGTG TGCCTCACTA TAACGAGCAG TGGCCCACGT ACGACCAGCA GCAGCAGCAG 
CAGACTTCCC CCCAGCCGCC ACCTGCGTCC GGCTTCCCTC AGGGCCACCA GCCTCCGCCT 
CCACCACCGA TGGGATATGA GCCACGCTCC CCATACGAGG ACGGACCTAA CAGGGATTTC 
AGCCCTCCTC AGTCCCAGTA TGACGGTGTG TCCCCAATGG GCTACGATCA CCGGCCGCGG 
CACCCCAAAC CTGCACCGGT CCGATATGAA GAGCCTCCAC CTCCACCTCC TCCGGTGTCC 
TACGATGCAC GGTCACCTTT CGAACCCGAG CCGCATGCTT TCCCAGGCAA CACACATCGC 
TCGCCAGACC CACCGAAACA GTATTATGGA GACGCCGCTA TGAGGCCCTT GTACAACCCT 
GGACCTCCGA ACAGAGTGTA CAAACATGAG CCGGTGAACT CTGAGCCTCC TGTACCGCCT 
CCCAAACCTG AATCTGTCCT GTCTCCAGGA GAACCGCCCC ATTCTGCAGC TTCCAAACCA 
TTGCCGCCTC CTCCCAGAGA TGATGACGAT GATGATGACG ATGATGATGA CCCTGCCATG 
AAGCCCCAGT CTGTGCTCAA CAGGGTCAAA ATGTTCGAAA ACAAACGCTC TGTGTCGGTA 
GACCGAGCCA AGGACGCACC TGACGTCACT GGAATTAGGC CCACAGATCT CCCCAAACCT 
GCGAGCACCC CTGGACCTGT GCTCAAAGCC AACTCTCTCA GCAACCTGGA GCAGGACAAA 
CCCTCCTACA GAGCTCCAGA GCCGCAGAAA CCTCAGACGC GTGTTGGGGA CGATGTGGTT 
CGCTCCAACC ACTACGACCC TGATGATGAT GAAGAGTACT ACAGAAAACA GCTCTCATTT 
TTCGACCGCC GCAGTTTCGA CAGTAAGGTC ATGAATCCAC CCAGCGCTGG AGTAAACCGC 
TTCCATGAGC CGCCCAAACC ACCCCAGACA CAGATCGCTT ATCCTTTCGC TAGGACGGAG 
TCTGTGGAGA AGGTGAGTCC TGTGGACAAG AGATACGAGC CTCTGCCGCC AGTCAACCCT 
TCACCTGCTC CATACAGCCA GAACACACCT GTCGCTCCGC CCACATCCCT GCCCAAACTC 
ACCAACATCG AGGTGAACTC TCTGCCAGAT CCTCACAGCT CTCCCAAAGC CAAACCGGAC 
CTGTCTGCTC TGAGAGCTCC GACCCGGGAC GAGCCCATCC AGAGCAGCTA CTTGCCCCCC 
AAGTCCACCA TCAATGGCAT GGATGCTCCT CCCAAAACAC TCGGCGTGCC GACCAGCTAC 
AACCGCTACG TCCCCAAGCC TTACACCAGC TCGGCGCGAC CCTTCGAGCG CAAGTTCGAG 
AGTCCGAAAT TCAACCACAA CCTGCTTCCC AACGACACGC AGTCCAAACC CAGTGTCAAC 
AACAACCTGA AGCCTCAGAT CTCCCCACAA CCCCAGGACA CGGACAGCGG CGTCGACACC 
TTCACTCGCA CCATGGACAA CAGGCCAAAA TATCAGCACA ACAACATCAA CGCCATTCCC 
AAAGCCGTCC CAGTCAGCCC CAGTGCACTG GATGATGATG AAGAAGATGA GGGACACACA 
GTTGTGGCCA CGGCGCGGGG CATCTTTAAC TGTAATGGCG GGGTGTTGAG CTCTATAGAG 
ACGGGCGTCA GCATCATTAT CCCACAGGGA GCCATTCCTG ATAGTGTGGA GCAGGAGATC 
TACTTCAAAG TGTGCCGGGA CAACAGCATC CTGCCTCCTC TGGATAAGGA GAAAGGTGAA 
ACTCTGCTGA GTCCGCTGGT GATGTGTGGT CCTCACGGCC TGAAGTTCCT GAAGCCTGTG 
GAGCTGCGCT TGCCACACTG TGACCCCAAA TGCTGGCAAA ACAAGTGTCT ACCAGGAGAC 
CCCAATTACC TGGTGGGCGC AAACTGTGTG TCTGTGCTGA TCGACCACTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296416.1
CDS	110..6103
Translation	MLLSVFGGPVEEVLAGLLKLKMFGSGSKKWFIKKNKRQRTKVQRKPASGRRNKRVSRVLKRHKEVIVKQLDVSKVLPTLVYERVFTLGEYKEILGQDSSGKRAELFLDLLSQKEPAAFSAFCGVLEEVCPHLLTCFLLDGTDGSAGENKKQGQTKPAHSQQNALSKGQDTVCFPPMYTDPLFDTRVFPKHDKDKYGPAVYYPKFNAYIPNPDKVSVLDESEGSGHGKPSLRRIKGRIHRSKSLDSIDLLDSNSAAMDETVIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGLLQENDRVVMVNAVSMDNVDHAYAVQQLRKSGKNAKITIRRKRKVQIPSGRPGERETMSEHDEDDDSYEDEIYESRSARSGPSAYSGGGGGGGGGGASGRRSGRGDRLSGRRERDRERSGSRERSLSPRSDRHSVSSNLPPRPAKVTLVKSRKNEEYGLRLASHIFVKDISPESLAARDGNIQEGDVVLKINGTVTENLSLIDAKKLIERSKGKLKMVVQRDERATLLNIPDLDDSIPSANASDRDDISDIHSVASDHSNRSHDRKRSSRSRSPDRRSEPSDHSRHSPPQISNGSHRSRDEDRISKAGPLPLPAKMVEEMPKEQSAARETEKQLPPLPEPKPVYAQPGQPDVDLPVSPADAPVPSVTHDDSILRPSMKLVKFKKGESVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFANIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHQEVERGIIPNKNRAEQLSSVQYTLPKTPGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVVIFGPIADVAREKLSREETDLFELAKSEPRDAGTDQRSSGIIRLHTIKQIIDRDKHAVLDITPNAVDRLNYAQWYPIVVFLNPDSKQGVKNMRTRLCPESRKSARKLYERALKLRKNNHHLFTTTINLNNMNDGWYGALKESIQQQQNQLVWVSEGKADGAPDDDLDIHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETMNDEVGLPSEPAITRSSEPVREDPPVIQDAAGYPSYQHAVPQPEAVSRIDPAGFKMAAPQQQAEAALASPPPPSAAVQPTATLAGTNPEEPPGTPQADSLNSPVPVPDPQPEPELAQPPTHDPHQSLPPGPDPKMYKKDLYSMDEPVRVTHGVKPPPPQQQQQPLGPPTSLSYSHQPVYQDQQPYRQYDHPPYGYDGGGYAQPKPHNYDPHLHYDNRVPHYNEQWPTYDQQQQQQTSPQPPPASGFPQGHQPPPPPPMGYEPRSPYEDGPNRDFSPPQSQYDGVSPMGYDHRPRHPKPAPVRYEEPPPPPPPVSYDARSPFEPEPHAFPGNTHRSPDPPKQYYGDAAMRPLYNPGPPNRVYKHEPVNSEPPVPPPKPESVLSPGEPPHSAASKPLPPPPRDDDDDDDDDDDPAMKPQSVLNRVKMFENKRSVSVDRAKDAPDVTGIRPTDLPKPASTPGPVLKANSLSNLEQDKPSYRAPEPQKPQTRVGDDVVRSNHYDPDDDEEYYRKQLSFFDRRSFDSKVMNPPSAGVNRFHEPPKPPQTQIAYPFARTESVEKVSPVDKRYEPLPPVNPSPAPYSQNTPVAPPTSLPKLTNIEVNSLPDPHSSPKAKPDLSALRAPTRDEPIQSSYLPPKSTINGMDAPPKTLGVPTSYNRYVPKPYTSSARPFERKFESPKFNHNLLPNDTQSKPSVNNNLKPQISPQPQDTDSGVDTFTRTMDNRPKYQHNNINAIPKAVPVSPSALDDDEEDEGHTVVATARGIFNCNGGVLSSIETGVSIIIPQGAIPDSVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCDPKCWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_009298141.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tight junction protein 1b (tjp1b), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298141.3

ORF Insert Sequence:

ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
TCCCGCAAGA ATGAAGAATA CGGGCTTCGC CTGGCCAGCC ACATCTTCGT GAAGGACATT 
TCCCCCGAGA GCCTGGCAGC CCGAGACGGA AACATCCAAG AGGGAGACGT GGTGCTTAAG 
ATTAATGGCA CGGTAACAGA GAATTTGTCC CTGATCGATG CTAAGAAGCT GATCGAGAGG 
TCAAAGGGAA AGCTGAAGAT GGTGGTGCAG AGAGACGAGC GGGCGACGCT GCTGAACATC 
CCCGATCTGG ACGACAGCAT ACCTTCTGCT AATGCCTCGG ACAGAGATGA TATTTCAGAC 
ATCCACTCCG TGGCCTCTGA TCATTCCAAC CGATCCCACG ACCGGAAGAG GAGCAGCCGC 
TCTCGTTCTC CAGACCGCCG CTCCGAACCC TCAGACCACT CCAGACACTC TCCACCACAG 
ATCAGCAACG GCAGTCACAG GAGCCGTGAT GAAGACAGGA TCTCCAAGGC CGGGCCGCTT 
CCTCTACCTG CTAAGATGGT GGAGGAGATG CCGAAAGAGC AGAGCGCAGC CAGAGAAACA 
GAGAAACAGC TCCCCCCTCT GCCAGAGCCC AAACCTGTGT ACGCTCAGCC GGGCCAGCCG 
GACGTGGACC TGCCTGTTAG TCCGGCAGAT GCTCCTGTGC CCAGCGTCAC ACATGATGAC 
AGCATCTTAC GGCCGAGCAT GAAGCTGGTG AAGTTCAAGA AGGGCGAGAG CGTGGGCTTG 
AGGCTCGCTG GGGGGAACGA CGTTGGGATA TTTGTGGCGG GAGTTCTGGA GGACAGTCCT 
GCTGCTAAAG AGGGCCTGGA AGAGGGAGAC CAGATTCTCA GGGTAAATAA TGTGGATTTT 
GCAAATATCA TCCGAGAGGA GGCAGTGCTG TTCCTGCTGG ATCTGCCCAA AGGAGAGGAG 
GTCACCATCC TGGCCCAGAA GAAGAAAGAC GTGTACCGCC GCATTGTAGA GTCTGACGTG 
GGAGACTCGT TCTACATCAG GACTCATTTT GAATATGAGA AGGAGTCGCC CTATGGCCTG 
AGCTTCAATA AGGGGGAGGT CTTCAGAGTC GTCGACACCC TTTACAATGG CAAACTGGGC 
TCCTGGCTCG CCATCCGCAT CGGCAAAAAC CACCAGGAGG TGGAGCGGGG CATCATCCCC 
AACAAGAACA GGGCTGAGCA GTTATCAAGT GTCCAGTACA CTCTCCCAAA GACTCCAGGA 
GGGGATCGCG CCGACTTCTG GAGATTCAGA GGCCTCCGCA GCTCCAAACG AAACCTGAGG 
AAGAGCAGAG AAGACCTGTC TGCTCAGCCG GTCCAAACCA AATTCCCTGC TTATGAAAGA 
GTGGTGCTTA GAGAAGCTGG TTTCTTGAGG CCAGTGGTCA TTTTCGGACC GATCGCGGAT 
GTGGCTAGAG AGAAGCTGTC CAGAGAGGAG ACTGACCTCT TCGAACTTGC AAAGAGTGAA 
CCCAGAGATG CGGGCACAGA CCAGCGCAGC TCCGGAATCA TCCGTCTCCA TACCATTAAA 
CAGATCATTG ACCGAGACAA GCACGCGGTG CTGGACATCA CCCCTAATGC GGTGGACCGT 
CTGAACTACG CTCAGTGGTA CCCCATCGTG GTGTTTCTGA ATCCAGACAG CAAACAGGGC 
GTCAAGAACA TGAGGACCAG GCTCTGTCCC GAGTCCAGGA AGAGCGCCCG AAAGCTCTAC 
GAGCGTGCTC TCAAACTGAG GAAGAACAAC CATCACCTTT TCACCACCAC TATTAACTTG 
AACAACATGA ATGACGGGTG GTACGGTGCT TTGAAAGAGA GCATCCAGCA GCAGCAGAAC 
CAGTTGGTCT GGGTCTCAGA GGGCAAGGCC GACGGCGCTC CTGACGATGA CCTTGACATC 
CATGACGACC GCCTCTCCTA CCTCTCGGCT CCGGGCAGCG AGTACAGCAT GTACAGCACC 
GACAGCCGCC ACACGTCCGA CTATGAGGAC ACCGACACGG AGGGTGGTGC CTACACCGAC 
CAGGAGCTGG ACGAGACCAT GAACGATGAG GTGGGCCTGC CTAGCGAGCC TGCCATCACA 
CGCTCATCTG AGCCTGTGCG AGAGGACCCG CCAGTCATTC AGGACGCAGC TGGATACCCC 
AGCTACCAGC ATGCGGTACC GCAACCTGAA GCGGTGAGCC GCATTGACCC GGCCGGGTTT 
AAGATGGCAG CGCCGCAGCA GCAAGCTGAG GCTGCTCTGG CCTCGCCGCC CCCTCCCTCT 
GCAGCGGTCC AGCCCACCGC AACACTAGCG GGTACGAACC CCGAGGAGCC GCCTGGCACC 
CCTCAGGCCG ACTCCCTTAA CAGCCCCGTC CCTGTTCCCG ACCCCCAGCC CGAACCTGAG 
CTCGCTCAGC CCCCAACACA CGACCCCCAC CAGTCGCTTC CTCCTGGCCC AGATCCAAAG 
ATGTATAAAA AGGATCTGTA CAGCATGGAC GAGCCGGTGC GAGTGACCCA CGGCGTGAAG 
CCTCCCCCTC CCCAGCAGCA GCAGCAGCCT TTAGGCCCCC CAACCTCGCT CTCCTACAGC 
CACCAGCCTG TCTACCAGGA CCAACAGCCA TACCGCCAGT ACGACCACCC GCCTTATGGC 
TATGACGGTG GCGGCTACGC ACAACCAAAG CCTCACAACT ACGACCCGCA CCTGCACTAC 
GACAACCGTG TGCCTCACTA TAACGAGCAG TGGCCCACGT ACGACCAGCA GCAGCAGCAG 
CAGACTTCCC CCCAGCCGCC ACCTGCGTCC GGCTTCCCTC AGGGCCACCA GCCTCCGCCT 
CCACCACCGA TGGGATATGA GCCACGCTCC CCATACGAGG ACGGACCTAA CAGGGATTTC 
AGCCCTCCTC AGTCCCAGTA TGACGGTGTG TCCCCAATGG GCTACGATCA CCGGCCGCGG 
CACCCCAAAC CTGCACCGGT CCGATATGAA GAGCCTCCAC CTCCACCTCC TCCGGTGTCC 
TACGATGCAC GGTCACCTTT CGAACCCGAG CCGCATGCTT TCCCAGGCAA CACACATCGC 
TCGCCAGACC CACCGAAACA GTATTATGGA GACGCCGCTA TGAGGCCCTT GTACAACCCT 
GGACCTCCGA ACAGAGTGTA CAAACATGAG CCGGTGAACT CTGAGCCTCC TGTACCGCCT 
CCCAAACCTG AATCTGTCCT GTCTCCAGGA GAACCGCCCC ATTCTGCAGC TTCCAAACCA 
TTGCCGCCTC CTCCCAGAGA TGATGACGAT GATGATGACG ATGATGATGA CCCTGCCATG 
AAGCCCCAGT CTGTGCTCAA CAGGGTCAAA ATGTTCGAAA ACAAACGCTC TGTGTCGGTA 
GACCGAGCCA AGGACGCACC TGACGTCACT GGAATTAGGC CCACAGATCT CCCCAAACCT 
GCGAGCACCC CTGGACCTGT GCTCAAAGCC AACTCTCTCA GCAACCTGGA GCAGGACAAA 
CCCTCCTACA GAGCTCCAGA GCCGCAGAAA CCTCAGACGC GTGTTGGGGA CGATGTGGTT 
CGCTCCAACC ACTACGACCC TGATGATGAT GAAGAGTACT ACAGAAAACA GCTCTCATTT 
TTCGACCGCC GCAGTTTCGA CAGTAAGGTC ATGAATCCAC CCAGCGCTGG AGTAAACCGC 
TTCCATGAGC CGCCCAAACC ACCCCAGACA CAGATCGCTT ATCCTTTCGC TAGGACGGAG 
TCTGTGGAGA AGGTGAGTCC TGTGGACAAG AGATACGAGC CTCTGCCGCC AGTCAACCCT 
TCACCTGCTC CATACAGCCA GAACACACCT GTCGCTCCGC CCACATCCCT GCCCAAACTC 
ACCAACATCG AGGTGAACTC TCTGCCAGAT CCTCACAGCT CTCCCAAAGC CAAACCGGAC 
CTGTCTGCTC TGAGAGCTCC GACCCGGGAC GAGCCCATCC AGAGCAGCTA CTTGCCCCCC 
AAGTCCACCA TCAATGGCAT GGATGCTCCT CCCAAAACAC TCGGCGTGCC GACCAGCTAC 
AACCGCTACG TCCCCAAGCC TTACACCAGC TCGGCGCGAC CCTTCGAGCG CAAGTTCGAG 
AGTCCGAAAT TCAACCACAA CCTGCTTCCC AACGACACGC AGTCCAAACC CAGTGTCAAC 
AACAACCTGA AGCCTCAGAT CTCCCCACAA CCCCAGGACA CGGACAGCGG CGTCGACACC 
TTCACTCGCA CCATGGACAA CAGGCCAAAA TATCAGCACA ACAACATCAA CGCCATTCCC 
AAAGCCGTCC CAGTCAGCCC CAGTGCACTG GATGATGATG AAGAAGATGA GGGACACACA 
GTTGTGGCCA CGGCGCGGGG CATCTTTAAC TGTAATGGCG GGGTGTTGAG CTCTATAGAG 
ACGGGCGTCA GCATCATTAT CCCACAGGGA GCCATTCCTG ATAGTGTGGA GCAGGAGATC 
TACTTCAAAG TGTGCCGGGA CAACAGCATC CTGCCTCCTC TGGATAAGGA GAAAGGTGAA 
ACTCTGCTGA GTCCGCTGGT GATGTGTGGT CCTCACGGCC TGAAGTTCCT GAAGCCTGTG 
GAGCTGCGCT TGCCACACTG TGACCCCAAA TGCTGGCAAA ACAAGTGTCT ACCAGGAGAC 
CCCAATTACC TGGTGGGCGC AAACTGTGTG TCTGTGCTGA TCGACCACTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp1b ( XM_017354168.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017209657.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X3, mRNA.

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CloneID	ODa57879
Clone ID Related Accession (Same CDS sequence)	XM_017354168.1
Accession Version	XM_017354168.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6084bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tight junction protein ZO-1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
TCCCGCAAGA ATGAAGCAGA ATACGGGCTT CGCCTGGCCA GCCACATCTT CGTGAAGGAC 
ATTTCCCCCG AGAGCCTGGC AGCCCGAGAC GGAAACATCC AAGAGGGAGA CGTGGTGCTT 
AAGATTAATG GCACGGTAAC AGAGAATTTG TCCCTGATCG ATGCTAAGAA GCTGATCGAG 
AGGTCAAAGG GAAAGCTGAA GATGGTGGTG CAGAGAGACG AGCGGGCGAC GCTGCTGAAC 
ATCCCCGATC TGGACGACAG CATACCTTCT GCTAATGCCT CGGACAGAGA TGACATCCAC 
TCCGTGGCCT CTGATCATTC CAACCGATCC CACGACCGGA AGAGGAGCAG CCGCTCTCGT 
TCTCCAGACC GCCGCTCCGA ACCCTCAGAC CACTCCAGAC ACTCTCCACC ACAGATCAGC 
AACGGCAGGG CCACAGGGCC GACGGCTCAG AGAACGCGGC CAGTTCACAG GAGCCGTGAT 
GAAGACAGGA TCTCCAAGGC CGGGCCGCTT CCTCTACCTG CTAAGATGGT GGAGGAGATG 
CCGAAAGAGC AGAGCGCAGC CAGAGAAACA GAGAAACAGC TCCCCCCTCT GCCAGAGCCC 
AAACCTGTGT ACGCTCAGCC GGGCCAGCCG GACGTGGACC TGCCTGTTAG TCCGGCAGAT 
GCTCCTGTGC CCAGCGTCAC ACATGATGAC AGCATCTTAC GGCCGAGCAT GAAGCTGGTG 
AAGTTCAAGA AGGGCGAGAG CGTGGGCTTG AGGCTCGCTG GGGGGAACGA CGTTGGGATA 
TTTGTGGCGG GAGTTCTGGA GGACAGTCCT GCTGCTAAAG AGGGCCTGGA AGAGGGAGAC 
CAGATTCTCA GGGTAAATAA TGTGGATTTT GCAAATATCA TCCGAGAGGA GGCAGTGCTG 
TTCCTGCTGG ATCTGCCCAA AGGAGAGGAG GTCACCATCC TGGCCCAGAA GAAGAAAGAC 
GTGTACCGCC GCATTGTAGA GTCTGACGTG GGAGACTCGT TCTACATCAG GACTCATTTT 
GAATATGAGA AGGAGTCGCC CTATGGCCTG AGCTTCAATA AGGGGGAGGT CTTCAGAGTC 
GTCGACACCC TTTACAATGG CAAACTGGGC TCCTGGCTCG CCATCCGCAT CGGCAAAAAC 
CACCAGGAGG TGGAGCGGGG CATCATCCCC AACAAGAACA GGGCTGAGCA GTTATCAAGT 
GTCCAGTACA CTCTCCCAAA GACTCCAGGA GGGGATCGCG CCGACTTCTG GAGATTCAGA 
GGCCTCCGCA GCTCCAAACG AAACCTGAGG AAGAGCAGAG AAGACCTGTC TGCTCAGCCG 
GTCCAAACCA AATTCCCTGC TTATGAAAGA GTGGTGCTTA GAGAAGCTGG TTTCTTGAGG 
CCAGTGGTCA TTTTCGGACC GATCGCGGAT GTGGCTAGAG AGAAGCTGTC CAGAGAGGAG 
ACTGACCTCT TCGAACTTGC AAAGAGTGAA CCCAGAGATG CGGGCACAGA CCAGCGCAGC 
TCCGGAATCA TCCGTCTCCA TACCATTAAA CAGATCATTG ACCGAGACAA GCACGCGGTG 
CTGGACATCA CCCCTAATGC GGTGGACCGT CTGAACTACG CTCAGTGGTA CCCCATCGTG 
GTGTTTCTGA ATCCAGACAG CAAACAGGGC GTCAAGAACA TGAGGACCAG GCTCTGTCCC 
GAGTCCAGGA AGAGCGCCCG AAAGCTCTAC GAGCGTGCTC TCAAACTGAG GAAGAACAAC 
CATCACCTTT TCACCACCAC TATTAACTTG AACAACATGA ATGACGGGTG GTACGGTGCT 
TTGAAAGAGA GCATCCAGCA GCAGCAGAAC CAGTTGGTCT GGGTCTCAGA GGGCAAGGCC 
GACGGCGCTC CTGACGATGA CCTTGACATC CATGACGACC GCCTCTCCTA CCTCTCGGCT 
CCGGGCAGCG AGTACAGCAT GTACAGCACC GACAGCCGCC ACACGTCCGA CTATGAGGAC 
ACCGACACGG AGGGTGGTGC CTACACCGAC CAGGAGCTGG ACGAGACCAT GAACGATGAG 
GTGGGCCTGC CTAGCGAGCC TGCCATCACA CGCTCATCTG AGCCTGTGCG AGAGGACCCG 
CCAGTCATTC AGGACGCAGC TGGATACCCC AGCTACCAGC ATGCGGTACC GCAACCTGAA 
GCGGTGAGCC GCATTGACCC GGCCGGGTTT AAGATGGCAG CGCCGCAGCA GCAAGCTGAG 
GCTGCTCTGG CCTCGCCGCC CCCTCCCTCT GCAGCGGTCC AGCCCACCGC AACACTAGCG 
GGTACGAACC CCGAGGAGCC GCCTGGCACC CCTCAGGCCG ACTCCCTTAA CAGCCCCGTC 
CCTGTTCCCG ACCCCCAGCC CGAACCTGAG CTCGCTCAGC CCCCAACACA CGACCCCCAC 
CAGTCGCTTC CTCCTGGCCC AGATCCAAAG ATGTATAAAA AGGATCTGTA CAGCATGGAC 
GAGCCGGTGC GAGTGACCCA CGGCGTGAAG CCTCCCCCTC CCCAGCAGCA GCAGCAGCCT 
TTAGGCCCCC CAACCTCGCT CTCCTACAGC CACCAGCCTG TCTACCAGGA CCAACAGCCA 
TACCGCCAGT ACGACCACCC GCCTTATGGC TATGACGGTG GCGGCTACGC ACAACCAAAG 
CCTCACAACT ACGACCCGCA CCTGCACTAC GACAACCGTG TGCCTCACTA TAACGAGCAG 
TGGCCCACGT ACGACCAGCA GCAGCAGCAG CAGACTTCCC CCCAGCCGCC ACCTGCGTCC 
GGCTTCCCTC AGGGCCACCA GCCTCCGCCT CCACCACCGA TGGGATATGA GCCACGCTCC 
CCATACGAGG ACGGACCTAA CAGGGATTTC AGCCCTCCTC AGTCCCAGTA TGACGGTGTG 
TCCCCAATGG GCTACGATCA CCGGCCGCGG CACCCCAAAC CTGCACCGGT CCGATATGAA 
GAGCCTCCAC CTCCACCTCC TCCGGTGTCC TACGATGCAC GGTCACCTTT CGAACCCGAG 
CCGCATGCTT TCCCAGGCAA CACACATCGC TCGCCAGACC CACCGAAACA GTATTATGGA 
GACGCCGCTA TGAGGCCCTT GTACAACCCT GGACCTCCGA ACAGAGTGTA CAAACATGAG 
CCGGTGAACT CTGAGCCTCC TGTACCGCCT CCCAAACCTG AATCTGTCCT GTCTCCAGGA 
GAACCGCCCC ATTCTGCAGC TTCCAAACCA TTGCCGCCTC CTCCCAGAGA TGATGACGAT 
GATGATGACG ATGATGATGA CCCTGCCATG AAGCCCCAGT CTGTGCTCAA CAGGGTCAAA 
ATGTTCGAAA ACAAACGCTC TGTGTCGGTA GACCGAGCCA AGGACGCACC TGACGTCACT 
GGAATTAGGC CCACAGATCT CCCCAAACCT GCGAGCACCC CTGGACCTGT GCTCAAAGCC 
AACTCTCTCA GCAACCTGGA GCAGGACAAA CCCTCCTACA GAGCTCCAGA GCCGCAGAAA 
CCTCAGACGC GTGTTGGGGA CGATGTGGTT CGCTCCAACC ACTACGACCC TGATGATGAT 
GAAGAGTACT ACAGAAAACA GCTCTCATTT TTCGACCGCC GCAGTTTCGA CAGTAAGGTC 
ATGAATCCAC CCAGCGCTGG AGTAAACCGC TTCCATGAGC CGCCCAAACC ACCCCAGACA 
CAGATCGCTT ATCCTTTCGC TAGGACGGAG TCTGTGGAGA AGGTGAGTCC TGTGGACAAG 
AGATACGAGC CTCTGCCGCC AGTCAACCCT TCACCTGCTC CATACAGCCA GAACACACCT 
GTCGCTCCGC CCACATCCCT GCCCAAACTC ACCAACATCG AGGTGAACTC TCTGCCAGAT 
CCTCACAGCT CTCCCAAAGC CAAACCGGAC CTGTCTGCTC TGAGAGCTCC GACCCGGGAC 
GAGCCCATCC AGAGCAGCTA CTTGCCCCCC AAGTCCACCA TCAATGGCAT GGATGCTCCT 
CCCAAAACAC TCGGCGTGCC GACCAGCTAC AACCGCTACG TCCCCAAGCC TTACACCAGC 
TCGGCGCGAC CCTTCGAGCG CAAGTTCGAG AGTCCGAAAT TCAACCACAA CCTGCTTCCC 
AACGACACGC AGTCCAAACC CAGTGTCAAC AACAACCTGA AGCCTCAGAT CTCCCCACAA 
CCCCAGGACA CGGACAGCGG CGTCGACACC TTCACTCGCA CCATGGACAA CAGGCCAAAA 
TATCAGCACA ACAACATCAA CGCCATTCCC AAAGCCGTCC CAGTCAGCCC CAGTGCACTG 
GATGATGATG AAGAAGATGA GGGACACACA GTTGTGGCCA CGGCGCGGGG CATCTTTAAC 
TGTAATGGCG GGGTGTTGAG CTCTATAGAG ACGGGCGTCA GCATCATTAT CCCACAGGGA 
GCCATTCCTG ATAGTGTGGA GCAGGAGATC TACTTCAAAG TGTGCCGGGA CAACAGCATC 
CTGCCTCCTC TGGATAAGGA GAAAGGTGAA ACTCTGCTGA GTCCGCTGGT GATGTGTGGT 
CCTCACGGCC TGAAGTTCCT GAAGCCTGTG GAGCTGCGCT TGCCACACTG TGCGTCTATG 
ACCCCTGATG GTTGGTCTTT TGCTCTAAAA TCCTCCGACT CCTCGTCGGG TGACCCCAAA 
TGCTGGCAAA ACAAGTGTCT ACCAGGAGAC CCCAATTACC TGGTGGGCGC AAACTGTGTG 
TCTGTGCTGA TCGACCACTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209657.1
CDS	115..6198
Translation	MLLSVFGGPVEEVLAGLLKLKMFGSGSKKWFIKKNKRQRTKVQRKPASGRRNKRVSRVLKRHKEVIVKQLDVSKVLPTLVYERVFTLGEYKEILGQDSSGKRAELFLDLLSQKEPAAFSAFCGVLEEVCPHLLTCFLLDGTDGSAGENKKQGQTKPAHSQQNALSKGQDTVCFPPMYTDPLFDTRVFPKHDKDKYGPAVYYPKFNAYIPNPDKVSVLDESEGSGHGKPSLRRIKGRIHRSKSLDSIDLLDSNSAAMDETVIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGLLQENDRVVMVNAVSMDNVDHAYAVQQLRKSGKNAKITIRRKRKVQIPSGRPGERETMSEHDEDDDSYEDEIYESRSARSGPSAYSGGGGGGGGGGASGRRSGRGDRLSGRRERDRERSGSRERSLSPRSDRHSVSSNLPPRPAKVTLVKSRKNEAEYGLRLASHIFVKDISPESLAARDGNIQEGDVVLKINGTVTENLSLIDAKKLIERSKGKLKMVVQRDERATLLNIPDLDDSIPSANASDRDDIHSVASDHSNRSHDRKRSSRSRSPDRRSEPSDHSRHSPPQISNGRATGPTAQRTRPVHRSRDEDRISKAGPLPLPAKMVEEMPKEQSAARETEKQLPPLPEPKPVYAQPGQPDVDLPVSPADAPVPSVTHDDSILRPSMKLVKFKKGESVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFANIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHQEVERGIIPNKNRAEQLSSVQYTLPKTPGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVVIFGPIADVAREKLSREETDLFELAKSEPRDAGTDQRSSGIIRLHTIKQIIDRDKHAVLDITPNAVDRLNYAQWYPIVVFLNPDSKQGVKNMRTRLCPESRKSARKLYERALKLRKNNHHLFTTTINLNNMNDGWYGALKESIQQQQNQLVWVSEGKADGAPDDDLDIHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETMNDEVGLPSEPAITRSSEPVREDPPVIQDAAGYPSYQHAVPQPEAVSRIDPAGFKMAAPQQQAEAALASPPPPSAAVQPTATLAGTNPEEPPGTPQADSLNSPVPVPDPQPEPELAQPPTHDPHQSLPPGPDPKMYKKDLYSMDEPVRVTHGVKPPPPQQQQQPLGPPTSLSYSHQPVYQDQQPYRQYDHPPYGYDGGGYAQPKPHNYDPHLHYDNRVPHYNEQWPTYDQQQQQQTSPQPPPASGFPQGHQPPPPPPMGYEPRSPYEDGPNRDFSPPQSQYDGVSPMGYDHRPRHPKPAPVRYEEPPPPPPPVSYDARSPFEPEPHAFPGNTHRSPDPPKQYYGDAAMRPLYNPGPPNRVYKHEPVNSEPPVPPPKPESVLSPGEPPHSAASKPLPPPPRDDDDDDDDDDDPAMKPQSVLNRVKMFENKRSVSVDRAKDAPDVTGIRPTDLPKPASTPGPVLKANSLSNLEQDKPSYRAPEPQKPQTRVGDDVVRSNHYDPDDDEEYYRKQLSFFDRRSFDSKVMNPPSAGVNRFHEPPKPPQTQIAYPFARTESVEKVSPVDKRYEPLPPVNPSPAPYSQNTPVAPPTSLPKLTNIEVNSLPDPHSSPKAKPDLSALRAPTRDEPIQSSYLPPKSTINGMDAPPKTLGVPTSYNRYVPKPYTSSARPFERKFESPKFNHNLLPNDTQSKPSVNNNLKPQISPQPQDTDSGVDTFTRTMDNRPKYQHNNINAIPKAVPVSPSALDDDEEDEGHTVVATARGIFNCNGGVLSSIETGVSIIIPQGAIPDSVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKCWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_017354168.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tight junction protein 1b (tjp1b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017354168.1

ORF Insert Sequence:

ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
TCCCGCAAGA ATGAAGCAGA ATACGGGCTT CGCCTGGCCA GCCACATCTT CGTGAAGGAC 
ATTTCCCCCG AGAGCCTGGC AGCCCGAGAC GGAAACATCC AAGAGGGAGA CGTGGTGCTT 
AAGATTAATG GCACGGTAAC AGAGAATTTG TCCCTGATCG ATGCTAAGAA GCTGATCGAG 
AGGTCAAAGG GAAAGCTGAA GATGGTGGTG CAGAGAGACG AGCGGGCGAC GCTGCTGAAC 
ATCCCCGATC TGGACGACAG CATACCTTCT GCTAATGCCT CGGACAGAGA TGACATCCAC 
TCCGTGGCCT CTGATCATTC CAACCGATCC CACGACCGGA AGAGGAGCAG CCGCTCTCGT 
TCTCCAGACC GCCGCTCCGA ACCCTCAGAC CACTCCAGAC ACTCTCCACC ACAGATCAGC 
AACGGCAGGG CCACAGGGCC GACGGCTCAG AGAACGCGGC CAGTTCACAG GAGCCGTGAT 
GAAGACAGGA TCTCCAAGGC CGGGCCGCTT CCTCTACCTG CTAAGATGGT GGAGGAGATG 
CCGAAAGAGC AGAGCGCAGC CAGAGAAACA GAGAAACAGC TCCCCCCTCT GCCAGAGCCC 
AAACCTGTGT ACGCTCAGCC GGGCCAGCCG GACGTGGACC TGCCTGTTAG TCCGGCAGAT 
GCTCCTGTGC CCAGCGTCAC ACATGATGAC AGCATCTTAC GGCCGAGCAT GAAGCTGGTG 
AAGTTCAAGA AGGGCGAGAG CGTGGGCTTG AGGCTCGCTG GGGGGAACGA CGTTGGGATA 
TTTGTGGCGG GAGTTCTGGA GGACAGTCCT GCTGCTAAAG AGGGCCTGGA AGAGGGAGAC 
CAGATTCTCA GGGTAAATAA TGTGGATTTT GCAAATATCA TCCGAGAGGA GGCAGTGCTG 
TTCCTGCTGG ATCTGCCCAA AGGAGAGGAG GTCACCATCC TGGCCCAGAA GAAGAAAGAC 
GTGTACCGCC GCATTGTAGA GTCTGACGTG GGAGACTCGT TCTACATCAG GACTCATTTT 
GAATATGAGA AGGAGTCGCC CTATGGCCTG AGCTTCAATA AGGGGGAGGT CTTCAGAGTC 
GTCGACACCC TTTACAATGG CAAACTGGGC TCCTGGCTCG CCATCCGCAT CGGCAAAAAC 
CACCAGGAGG TGGAGCGGGG CATCATCCCC AACAAGAACA GGGCTGAGCA GTTATCAAGT 
GTCCAGTACA CTCTCCCAAA GACTCCAGGA GGGGATCGCG CCGACTTCTG GAGATTCAGA 
GGCCTCCGCA GCTCCAAACG AAACCTGAGG AAGAGCAGAG AAGACCTGTC TGCTCAGCCG 
GTCCAAACCA AATTCCCTGC TTATGAAAGA GTGGTGCTTA GAGAAGCTGG TTTCTTGAGG 
CCAGTGGTCA TTTTCGGACC GATCGCGGAT GTGGCTAGAG AGAAGCTGTC CAGAGAGGAG 
ACTGACCTCT TCGAACTTGC AAAGAGTGAA CCCAGAGATG CGGGCACAGA CCAGCGCAGC 
TCCGGAATCA TCCGTCTCCA TACCATTAAA CAGATCATTG ACCGAGACAA GCACGCGGTG 
CTGGACATCA CCCCTAATGC GGTGGACCGT CTGAACTACG CTCAGTGGTA CCCCATCGTG 
GTGTTTCTGA ATCCAGACAG CAAACAGGGC GTCAAGAACA TGAGGACCAG GCTCTGTCCC 
GAGTCCAGGA AGAGCGCCCG AAAGCTCTAC GAGCGTGCTC TCAAACTGAG GAAGAACAAC 
CATCACCTTT TCACCACCAC TATTAACTTG AACAACATGA ATGACGGGTG GTACGGTGCT 
TTGAAAGAGA GCATCCAGCA GCAGCAGAAC CAGTTGGTCT GGGTCTCAGA GGGCAAGGCC 
GACGGCGCTC CTGACGATGA CCTTGACATC CATGACGACC GCCTCTCCTA CCTCTCGGCT 
CCGGGCAGCG AGTACAGCAT GTACAGCACC GACAGCCGCC ACACGTCCGA CTATGAGGAC 
ACCGACACGG AGGGTGGTGC CTACACCGAC CAGGAGCTGG ACGAGACCAT GAACGATGAG 
GTGGGCCTGC CTAGCGAGCC TGCCATCACA CGCTCATCTG AGCCTGTGCG AGAGGACCCG 
CCAGTCATTC AGGACGCAGC TGGATACCCC AGCTACCAGC ATGCGGTACC GCAACCTGAA 
GCGGTGAGCC GCATTGACCC GGCCGGGTTT AAGATGGCAG CGCCGCAGCA GCAAGCTGAG 
GCTGCTCTGG CCTCGCCGCC CCCTCCCTCT GCAGCGGTCC AGCCCACCGC AACACTAGCG 
GGTACGAACC CCGAGGAGCC GCCTGGCACC CCTCAGGCCG ACTCCCTTAA CAGCCCCGTC 
CCTGTTCCCG ACCCCCAGCC CGAACCTGAG CTCGCTCAGC CCCCAACACA CGACCCCCAC 
CAGTCGCTTC CTCCTGGCCC AGATCCAAAG ATGTATAAAA AGGATCTGTA CAGCATGGAC 
GAGCCGGTGC GAGTGACCCA CGGCGTGAAG CCTCCCCCTC CCCAGCAGCA GCAGCAGCCT 
TTAGGCCCCC CAACCTCGCT CTCCTACAGC CACCAGCCTG TCTACCAGGA CCAACAGCCA 
TACCGCCAGT ACGACCACCC GCCTTATGGC TATGACGGTG GCGGCTACGC ACAACCAAAG 
CCTCACAACT ACGACCCGCA CCTGCACTAC GACAACCGTG TGCCTCACTA TAACGAGCAG 
TGGCCCACGT ACGACCAGCA GCAGCAGCAG CAGACTTCCC CCCAGCCGCC ACCTGCGTCC 
GGCTTCCCTC AGGGCCACCA GCCTCCGCCT CCACCACCGA TGGGATATGA GCCACGCTCC 
CCATACGAGG ACGGACCTAA CAGGGATTTC AGCCCTCCTC AGTCCCAGTA TGACGGTGTG 
TCCCCAATGG GCTACGATCA CCGGCCGCGG CACCCCAAAC CTGCACCGGT CCGATATGAA 
GAGCCTCCAC CTCCACCTCC TCCGGTGTCC TACGATGCAC GGTCACCTTT CGAACCCGAG 
CCGCATGCTT TCCCAGGCAA CACACATCGC TCGCCAGACC CACCGAAACA GTATTATGGA 
GACGCCGCTA TGAGGCCCTT GTACAACCCT GGACCTCCGA ACAGAGTGTA CAAACATGAG 
CCGGTGAACT CTGAGCCTCC TGTACCGCCT CCCAAACCTG AATCTGTCCT GTCTCCAGGA 
GAACCGCCCC ATTCTGCAGC TTCCAAACCA TTGCCGCCTC CTCCCAGAGA TGATGACGAT 
GATGATGACG ATGATGATGA CCCTGCCATG AAGCCCCAGT CTGTGCTCAA CAGGGTCAAA 
ATGTTCGAAA ACAAACGCTC TGTGTCGGTA GACCGAGCCA AGGACGCACC TGACGTCACT 
GGAATTAGGC CCACAGATCT CCCCAAACCT GCGAGCACCC CTGGACCTGT GCTCAAAGCC 
AACTCTCTCA GCAACCTGGA GCAGGACAAA CCCTCCTACA GAGCTCCAGA GCCGCAGAAA 
CCTCAGACGC GTGTTGGGGA CGATGTGGTT CGCTCCAACC ACTACGACCC TGATGATGAT 
GAAGAGTACT ACAGAAAACA GCTCTCATTT TTCGACCGCC GCAGTTTCGA CAGTAAGGTC 
ATGAATCCAC CCAGCGCTGG AGTAAACCGC TTCCATGAGC CGCCCAAACC ACCCCAGACA 
CAGATCGCTT ATCCTTTCGC TAGGACGGAG TCTGTGGAGA AGGTGAGTCC TGTGGACAAG 
AGATACGAGC CTCTGCCGCC AGTCAACCCT TCACCTGCTC CATACAGCCA GAACACACCT 
GTCGCTCCGC CCACATCCCT GCCCAAACTC ACCAACATCG AGGTGAACTC TCTGCCAGAT 
CCTCACAGCT CTCCCAAAGC CAAACCGGAC CTGTCTGCTC TGAGAGCTCC GACCCGGGAC 
GAGCCCATCC AGAGCAGCTA CTTGCCCCCC AAGTCCACCA TCAATGGCAT GGATGCTCCT 
CCCAAAACAC TCGGCGTGCC GACCAGCTAC AACCGCTACG TCCCCAAGCC TTACACCAGC 
TCGGCGCGAC CCTTCGAGCG CAAGTTCGAG AGTCCGAAAT TCAACCACAA CCTGCTTCCC 
AACGACACGC AGTCCAAACC CAGTGTCAAC AACAACCTGA AGCCTCAGAT CTCCCCACAA 
CCCCAGGACA CGGACAGCGG CGTCGACACC TTCACTCGCA CCATGGACAA CAGGCCAAAA 
TATCAGCACA ACAACATCAA CGCCATTCCC AAAGCCGTCC CAGTCAGCCC CAGTGCACTG 
GATGATGATG AAGAAGATGA GGGACACACA GTTGTGGCCA CGGCGCGGGG CATCTTTAAC 
TGTAATGGCG GGGTGTTGAG CTCTATAGAG ACGGGCGTCA GCATCATTAT CCCACAGGGA 
GCCATTCCTG ATAGTGTGGA GCAGGAGATC TACTTCAAAG TGTGCCGGGA CAACAGCATC 
CTGCCTCCTC TGGATAAGGA GAAAGGTGAA ACTCTGCTGA GTCCGCTGGT GATGTGTGGT 
CCTCACGGCC TGAAGTTCCT GAAGCCTGTG GAGCTGCGCT TGCCACACTG TGCGTCTATG 
ACCCCTGATG GTTGGTCTTT TGCTCTAAAA TCCTCCGACT CCTCGTCGGG TGACCCCAAA 
TGCTGGCAAA ACAAGTGTCT ACCAGGAGAC CCCAATTACC TGGTGGGCGC AAACTGTGTG 
TCTGTGCTGA TCGACCACTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp1b ( XM_017354169.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017209658.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X4, mRNA.

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CloneID	ODa57880
Clone ID Related Accession (Same CDS sequence)	XM_017354169.1
Accession Version	XM_017354169.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6078bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tight junction protein ZO-1 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
TCCCGCAAGA ATGAAGCAGA ATACGGGCTT CGCCTGGCCA GCCACATCTT CGTGAAGGAC 
ATTTCCCCCG AGAGCCTGGC AGCCCGAGAC GGAAACATCC AAGAGGGAGA CGTGGTGCTT 
AAGATTAATG GCACGGTAAC AGAGAATTTG TCCCTGATCG ATGCTAAGAA GCTGATCGAG 
AGGTCAAAGG GAAAGCTGAA GATGGTGGTG CAGAGAGACG AGCGGGCGAC GCTGCTGAAC 
ATCCCCGATC TGGACGACAG CATACCTTCT GCTAATGCCT CGGACAGAGA TGATATTTCA 
GACATCCACT CCGTGGCCTC TGATCATTCC AACCGATCCC ACGACCGGAA GAGGAGCAGC 
CGCTCTCGTT CTCCAGACCG CCGCTCCGAA CCCTCAGACC ACTCCAGACA CTCTCCACCA 
CAGATCAGCA ACGGCAGGGC CACAGGGCCG ACGGCTCAGA GAACGCGGCC AGTTCACAGG 
AGCCGTGATG AAGACAGGAT CTCCAAGGCC GGGCCGCTTC CTCTACCTGC TAAGATGGTG 
GAGGAGATGC CGAAAGAGCA GAGCGCAGCC AGAGAAACAG AGAAACAGCT CCCCCCTCTG 
CCAGAGCCCA AACCTGTGTA CGCTCAGCCG GGCCAGCCGG ACGTGGACCT GCCTGTTAGT 
CCGGCAGATG CTCCTGTGCC CAGCGTCACA CATGATGACA GCATCTTACG GCCGAGCATG 
AAGCTGGTGA AGTTCAAGAA GGGCGAGAGC GTGGGCTTGA GGCTCGCTGG GGGGAACGAC 
GTTGGGATAT TTGTGGCGGG AGTTCTGGAG GACAGTCCTG CTGCTAAAGA GGGCCTGGAA 
GAGGGAGACC AGATTCTCAG GGTAAATAAT GTGGATTTTG CAAATATCAT CCGAGAGGAG 
GCAGTGCTGT TCCTGCTGGA TCTGCCCAAA GGAGAGGAGG TCACCATCCT GGCCCAGAAG 
AAGAAAGACG TGTACCGCCG CATTGTAGAG TCTGACGTGG GAGACTCGTT CTACATCAGG 
ACTCATTTTG AATATGAGAA GGAGTCGCCC TATGGCCTGA GCTTCAATAA GGGGGAGGTC 
TTCAGAGTCG TCGACACCCT TTACAATGGC AAACTGGGCT CCTGGCTCGC CATCCGCATC 
GGCAAAAACC ACCAGGAGGT GGAGCGGGGC ATCATCCCCA ACAAGAACAG GGCTGAGCAG 
TTATCAAGTG TCCAGTACAC TCTCCCAAAG ACTCCAGGAG GGGATCGCGC CGACTTCTGG 
AGATTCAGAG GCCTCCGCAG CTCCAAACGA AACCTGAGGA AGAGCAGAGA AGACCTGTCT 
GCTCAGCCGG TCCAAACCAA ATTCCCTGCT TATGAAAGAG TGGTGCTTAG AGAAGCTGGT 
TTCTTGAGGC CAGTGGTCAT TTTCGGACCG ATCGCGGATG TGGCTAGAGA GAAGCTGTCC 
AGAGAGGAGA CTGACCTCTT CGAACTTGCA AAGAGTGAAC CCAGAGATGC GGGCACAGAC 
CAGCGCAGCT CCGGAATCAT CCGTCTCCAT ACCATTAAAC AGATCATTGA CCGAGACAAG 
CACGCGGTGC TGGACATCAC CCCTAATGCG GTGGACCGTC TGAACTACGC TCAGTGGTAC 
CCCATCGTGG TGTTTCTGAA TCCAGACAGC AAACAGGGCG TCAAGAACAT GAGGACCAGG 
CTCTGTCCCG AGTCCAGGAA GAGCGCCCGA AAGCTCTACG AGCGTGCTCT CAAACTGAGG 
AAGAACAACC ATCACCTTTT CACCACCACT ATTAACTTGA ACAACATGAA TGACGGGTGG 
TACGGTGCTT TGAAAGAGAG CATCCAGCAG CAGCAGAACC AGTTGGTCTG GGTCTCAGAG 
GGCAAGGCCG ACGGCGCTCC TGACGATGAC CTTGACATCC ATGACGACCG CCTCTCCTAC 
CTCTCGGCTC CGGGCAGCGA GTACAGCATG TACAGCACCG ACAGCCGCCA CACGTCCGAC 
TATGAGGACA CCGACACGGA GGGTGGTGCC TACACCGACC AGGAGCTGGA CGAGACCATG 
AACGATGAGG TGGGCCTGCC TAGCGAGCCT GCCATCACAC GCTCATCTGA GCCTGTGCGA 
GAGGACCCGC CAGTCATTCA GGACGCAGCT GGATACCCCA GCTACCAGCA TGCGGTACCG 
CAACCTGAAG CGGTGAGCCG CATTGACCCG GCCGGGTTTA AGATGGCAGC GCCGCAGCAG 
CAAGCTGAGG CTGCTCTGGC CTCGCCGCCC CCTCCCTCTG CAGCGGTCCA GCCCACCGCA 
ACACTAGCGG GTACGAACCC CGAGGAGCCG CCTGGCACCC CTCAGGCCGA CTCCCTTAAC 
AGCCCCGTCC CTGTTCCCGA CCCCCAGCCC GAACCTGAGC TCGCTCAGCC CCCAACACAC 
GACCCCCACC AGTCGCTTCC TCCTGGCCCA GATCCAAAGA TGTATAAAAA GGATCTGTAC 
AGCATGGACG AGCCGGTGCG AGTGACCCAC GGCGTGAAGC CTCCCCCTCC CCAGCAGCAG 
CAGCAGCCTT TAGGCCCCCC AACCTCGCTC TCCTACAGCC ACCAGCCTGT CTACCAGGAC 
CAACAGCCAT ACCGCCAGTA CGACCACCCG CCTTATGGCT ATGACGGTGG CGGCTACGCA 
CAACCAAAGC CTCACAACTA CGACCCGCAC CTGCACTACG ACAACCGTGT GCCTCACTAT 
AACGAGCAGT GGCCCACGTA CGACCAGCAG CAGCAGCAGC AGACTTCCCC CCAGCCGCCA 
CCTGCGTCCG GCTTCCCTCA GGGCCACCAG CCTCCGCCTC CACCACCGAT GGGATATGAG 
CCACGCTCCC CATACGAGGA CGGACCTAAC AGGGATTTCA GCCCTCCTCA GTCCCAGTAT 
GACGGTGTGT CCCCAATGGG CTACGATCAC CGGCCGCGGC ACCCCAAACC TGCACCGGTC 
CGATATGAAG AGCCTCCACC TCCACCTCCT CCGGTGTCCT ACGATGCACG GTCACCTTTC 
GAACCCGAGC CGCATGCTTT CCCAGGCAAC ACACATCGCT CGCCAGACCC ACCGAAACAG 
TATTATGGAG ACGCCGCTAT GAGGCCCTTG TACAACCCTG GACCTCCGAA CAGAGTGTAC 
AAACATGAGC CGGTGAACTC TGAGCCTCCT GTACCGCCTC CCAAACCTGA ATCTGTCCTG 
TCTCCAGGAG AACCGCCCCA TTCTGCAGCT TCCAAACCAT TGCCGCCTCC TCCCAGAGAT 
GATGACGATG ATGATGACGA TGATGATGAC CCTGCCATGA AGCCCCAGTC TGTGCTCAAC 
AGGGTCAAAA TGTTCGAAAA CAAACGCTCT GTGTCGGTAG ACCGAGCCAA GGACGCACCT 
GACGTCACTG GAATTAGGCC CACAGATCTC CCCAAACCTG CGAGCACCCC TGGACCTGTG 
CTCAAAGCCA ACTCTCTCAG CAACCTGGAG CAGGACAAAC CCTCCTACAG AGCTCCAGAG 
CCGCAGAAAC CTCAGACGCG TGTTGGGGAC GATGTGGTTC GCTCCAACCA CTACGACCCT 
GATGATGATG AAGAGTACTA CAGAAAACAG CTCTCATTTT TCGACCGCCG CAGTTTCGAC 
AGTAAGGTCA TGAATCCACC CAGCGCTGGA GTAAACCGCT TCCATGAGCC GCCCAAACCA 
CCCCAGACAC AGATCGCTTA TCCTTTCGCT AGGACGGAGT CTGTGGAGAA GGTGAGTCCT 
GTGGACAAGA GATACGAGCC TCTGCCGCCA GTCAACCCTT CACCTGCTCC ATACAGCCAG 
AACACACCTG TCGCTCCGCC CACATCCCTG CCCAAACTCA CCAACATCGA GGATCCTCAC 
AGCTCTCCCA AAGCCAAACC GGACCTGTCT GCTCTGAGAG CTCCGACCCG GGACGAGCCC 
ATCCAGAGCA GCTACTTGCC CCCCAAGTCC ACCATCAATG GCATGGATGC TCCTCCCAAA 
ACACTCGGCG TGCCGACCAG CTACAACCGC TACGTCCCCA AGCCTTACAC CAGCTCGGCG 
CGACCCTTCG AGCGCAAGTT CGAGAGTCCG AAATTCAACC ACAACCTGCT TCCCAACGAC 
ACGCAGTCCA AACCCAGTGT CAACAACAAC CTGAAGCCTC AGATCTCCCC ACAACCCCAG 
GACACGGACA GCGGCGTCGA CACCTTCACT CGCACCATGG ACAACAGGCC AAAATATCAG 
CACAACAACA TCAACGCCAT TCCCAAAGCC GTCCCAGTCA GCCCCAGTGC ACTGGATGAT 
GATGAAGAAG ATGAGGGACA CACAGTTGTG GCCACGGCGC GGGGCATCTT TAACTGTAAT 
GGCGGGGTGT TGAGCTCTAT AGAGACGGGC GTCAGCATCA TTATCCCACA GGGAGCCATT 
CCTGATAGTG TGGAGCAGGA GATCTACTTC AAAGTGTGCC GGGACAACAG CATCCTGCCT 
CCTCTGGATA AGGAGAAAGG TGAAACTCTG CTGAGTCCGC TGGTGATGTG TGGTCCTCAC 
GGCCTGAAGT TCCTGAAGCC TGTGGAGCTG CGCTTGCCAC ACTGTGCGTC TATGACCCCT 
GATGGTTGGT CTTTTGCTCT AAAATCCTCC GACTCCTCGT CGGGTGACCC CAAATGCTGG 
CAAAACAAGT GTCTACCAGG AGACCCCAAT TACCTGGTGG GCGCAAACTG TGTGTCTGTG 
CTGATCGACC ACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209658.1
CDS	115..6192
Translation	MLLSVFGGPVEEVLAGLLKLKMFGSGSKKWFIKKNKRQRTKVQRKPASGRRNKRVSRVLKRHKEVIVKQLDVSKVLPTLVYERVFTLGEYKEILGQDSSGKRAELFLDLLSQKEPAAFSAFCGVLEEVCPHLLTCFLLDGTDGSAGENKKQGQTKPAHSQQNALSKGQDTVCFPPMYTDPLFDTRVFPKHDKDKYGPAVYYPKFNAYIPNPDKVSVLDESEGSGHGKPSLRRIKGRIHRSKSLDSIDLLDSNSAAMDETVIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGLLQENDRVVMVNAVSMDNVDHAYAVQQLRKSGKNAKITIRRKRKVQIPSGRPGERETMSEHDEDDDSYEDEIYESRSARSGPSAYSGGGGGGGGGGASGRRSGRGDRLSGRRERDRERSGSRERSLSPRSDRHSVSSNLPPRPAKVTLVKSRKNEAEYGLRLASHIFVKDISPESLAARDGNIQEGDVVLKINGTVTENLSLIDAKKLIERSKGKLKMVVQRDERATLLNIPDLDDSIPSANASDRDDISDIHSVASDHSNRSHDRKRSSRSRSPDRRSEPSDHSRHSPPQISNGRATGPTAQRTRPVHRSRDEDRISKAGPLPLPAKMVEEMPKEQSAARETEKQLPPLPEPKPVYAQPGQPDVDLPVSPADAPVPSVTHDDSILRPSMKLVKFKKGESVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFANIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHQEVERGIIPNKNRAEQLSSVQYTLPKTPGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVVIFGPIADVAREKLSREETDLFELAKSEPRDAGTDQRSSGIIRLHTIKQIIDRDKHAVLDITPNAVDRLNYAQWYPIVVFLNPDSKQGVKNMRTRLCPESRKSARKLYERALKLRKNNHHLFTTTINLNNMNDGWYGALKESIQQQQNQLVWVSEGKADGAPDDDLDIHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETMNDEVGLPSEPAITRSSEPVREDPPVIQDAAGYPSYQHAVPQPEAVSRIDPAGFKMAAPQQQAEAALASPPPPSAAVQPTATLAGTNPEEPPGTPQADSLNSPVPVPDPQPEPELAQPPTHDPHQSLPPGPDPKMYKKDLYSMDEPVRVTHGVKPPPPQQQQQPLGPPTSLSYSHQPVYQDQQPYRQYDHPPYGYDGGGYAQPKPHNYDPHLHYDNRVPHYNEQWPTYDQQQQQQTSPQPPPASGFPQGHQPPPPPPMGYEPRSPYEDGPNRDFSPPQSQYDGVSPMGYDHRPRHPKPAPVRYEEPPPPPPPVSYDARSPFEPEPHAFPGNTHRSPDPPKQYYGDAAMRPLYNPGPPNRVYKHEPVNSEPPVPPPKPESVLSPGEPPHSAASKPLPPPPRDDDDDDDDDDDPAMKPQSVLNRVKMFENKRSVSVDRAKDAPDVTGIRPTDLPKPASTPGPVLKANSLSNLEQDKPSYRAPEPQKPQTRVGDDVVRSNHYDPDDDEEYYRKQLSFFDRRSFDSKVMNPPSAGVNRFHEPPKPPQTQIAYPFARTESVEKVSPVDKRYEPLPPVNPSPAPYSQNTPVAPPTSLPKLTNIEDPHSSPKAKPDLSALRAPTRDEPIQSSYLPPKSTINGMDAPPKTLGVPTSYNRYVPKPYTSSARPFERKFESPKFNHNLLPNDTQSKPSVNNNLKPQISPQPQDTDSGVDTFTRTMDNRPKYQHNNINAIPKAVPVSPSALDDDEEDEGHTVVATARGIFNCNGGVLSSIETGVSIIIPQGAIPDSVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKCWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_017354169.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tight junction protein 1b (tjp1b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017354169.1

ORF Insert Sequence:

ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
TCCCGCAAGA ATGAAGCAGA ATACGGGCTT CGCCTGGCCA GCCACATCTT CGTGAAGGAC 
ATTTCCCCCG AGAGCCTGGC AGCCCGAGAC GGAAACATCC AAGAGGGAGA CGTGGTGCTT 
AAGATTAATG GCACGGTAAC AGAGAATTTG TCCCTGATCG ATGCTAAGAA GCTGATCGAG 
AGGTCAAAGG GAAAGCTGAA GATGGTGGTG CAGAGAGACG AGCGGGCGAC GCTGCTGAAC 
ATCCCCGATC TGGACGACAG CATACCTTCT GCTAATGCCT CGGACAGAGA TGATATTTCA 
GACATCCACT CCGTGGCCTC TGATCATTCC AACCGATCCC ACGACCGGAA GAGGAGCAGC 
CGCTCTCGTT CTCCAGACCG CCGCTCCGAA CCCTCAGACC ACTCCAGACA CTCTCCACCA 
CAGATCAGCA ACGGCAGGGC CACAGGGCCG ACGGCTCAGA GAACGCGGCC AGTTCACAGG 
AGCCGTGATG AAGACAGGAT CTCCAAGGCC GGGCCGCTTC CTCTACCTGC TAAGATGGTG 
GAGGAGATGC CGAAAGAGCA GAGCGCAGCC AGAGAAACAG AGAAACAGCT CCCCCCTCTG 
CCAGAGCCCA AACCTGTGTA CGCTCAGCCG GGCCAGCCGG ACGTGGACCT GCCTGTTAGT 
CCGGCAGATG CTCCTGTGCC CAGCGTCACA CATGATGACA GCATCTTACG GCCGAGCATG 
AAGCTGGTGA AGTTCAAGAA GGGCGAGAGC GTGGGCTTGA GGCTCGCTGG GGGGAACGAC 
GTTGGGATAT TTGTGGCGGG AGTTCTGGAG GACAGTCCTG CTGCTAAAGA GGGCCTGGAA 
GAGGGAGACC AGATTCTCAG GGTAAATAAT GTGGATTTTG CAAATATCAT CCGAGAGGAG 
GCAGTGCTGT TCCTGCTGGA TCTGCCCAAA GGAGAGGAGG TCACCATCCT GGCCCAGAAG 
AAGAAAGACG TGTACCGCCG CATTGTAGAG TCTGACGTGG GAGACTCGTT CTACATCAGG 
ACTCATTTTG AATATGAGAA GGAGTCGCCC TATGGCCTGA GCTTCAATAA GGGGGAGGTC 
TTCAGAGTCG TCGACACCCT TTACAATGGC AAACTGGGCT CCTGGCTCGC CATCCGCATC 
GGCAAAAACC ACCAGGAGGT GGAGCGGGGC ATCATCCCCA ACAAGAACAG GGCTGAGCAG 
TTATCAAGTG TCCAGTACAC TCTCCCAAAG ACTCCAGGAG GGGATCGCGC CGACTTCTGG 
AGATTCAGAG GCCTCCGCAG CTCCAAACGA AACCTGAGGA AGAGCAGAGA AGACCTGTCT 
GCTCAGCCGG TCCAAACCAA ATTCCCTGCT TATGAAAGAG TGGTGCTTAG AGAAGCTGGT 
TTCTTGAGGC CAGTGGTCAT TTTCGGACCG ATCGCGGATG TGGCTAGAGA GAAGCTGTCC 
AGAGAGGAGA CTGACCTCTT CGAACTTGCA AAGAGTGAAC CCAGAGATGC GGGCACAGAC 
CAGCGCAGCT CCGGAATCAT CCGTCTCCAT ACCATTAAAC AGATCATTGA CCGAGACAAG 
CACGCGGTGC TGGACATCAC CCCTAATGCG GTGGACCGTC TGAACTACGC TCAGTGGTAC 
CCCATCGTGG TGTTTCTGAA TCCAGACAGC AAACAGGGCG TCAAGAACAT GAGGACCAGG 
CTCTGTCCCG AGTCCAGGAA GAGCGCCCGA AAGCTCTACG AGCGTGCTCT CAAACTGAGG 
AAGAACAACC ATCACCTTTT CACCACCACT ATTAACTTGA ACAACATGAA TGACGGGTGG 
TACGGTGCTT TGAAAGAGAG CATCCAGCAG CAGCAGAACC AGTTGGTCTG GGTCTCAGAG 
GGCAAGGCCG ACGGCGCTCC TGACGATGAC CTTGACATCC ATGACGACCG CCTCTCCTAC 
CTCTCGGCTC CGGGCAGCGA GTACAGCATG TACAGCACCG ACAGCCGCCA CACGTCCGAC 
TATGAGGACA CCGACACGGA GGGTGGTGCC TACACCGACC AGGAGCTGGA CGAGACCATG 
AACGATGAGG TGGGCCTGCC TAGCGAGCCT GCCATCACAC GCTCATCTGA GCCTGTGCGA 
GAGGACCCGC CAGTCATTCA GGACGCAGCT GGATACCCCA GCTACCAGCA TGCGGTACCG 
CAACCTGAAG CGGTGAGCCG CATTGACCCG GCCGGGTTTA AGATGGCAGC GCCGCAGCAG 
CAAGCTGAGG CTGCTCTGGC CTCGCCGCCC CCTCCCTCTG CAGCGGTCCA GCCCACCGCA 
ACACTAGCGG GTACGAACCC CGAGGAGCCG CCTGGCACCC CTCAGGCCGA CTCCCTTAAC 
AGCCCCGTCC CTGTTCCCGA CCCCCAGCCC GAACCTGAGC TCGCTCAGCC CCCAACACAC 
GACCCCCACC AGTCGCTTCC TCCTGGCCCA GATCCAAAGA TGTATAAAAA GGATCTGTAC 
AGCATGGACG AGCCGGTGCG AGTGACCCAC GGCGTGAAGC CTCCCCCTCC CCAGCAGCAG 
CAGCAGCCTT TAGGCCCCCC AACCTCGCTC TCCTACAGCC ACCAGCCTGT CTACCAGGAC 
CAACAGCCAT ACCGCCAGTA CGACCACCCG CCTTATGGCT ATGACGGTGG CGGCTACGCA 
CAACCAAAGC CTCACAACTA CGACCCGCAC CTGCACTACG ACAACCGTGT GCCTCACTAT 
AACGAGCAGT GGCCCACGTA CGACCAGCAG CAGCAGCAGC AGACTTCCCC CCAGCCGCCA 
CCTGCGTCCG GCTTCCCTCA GGGCCACCAG CCTCCGCCTC CACCACCGAT GGGATATGAG 
CCACGCTCCC CATACGAGGA CGGACCTAAC AGGGATTTCA GCCCTCCTCA GTCCCAGTAT 
GACGGTGTGT CCCCAATGGG CTACGATCAC CGGCCGCGGC ACCCCAAACC TGCACCGGTC 
CGATATGAAG AGCCTCCACC TCCACCTCCT CCGGTGTCCT ACGATGCACG GTCACCTTTC 
GAACCCGAGC CGCATGCTTT CCCAGGCAAC ACACATCGCT CGCCAGACCC ACCGAAACAG 
TATTATGGAG ACGCCGCTAT GAGGCCCTTG TACAACCCTG GACCTCCGAA CAGAGTGTAC 
AAACATGAGC CGGTGAACTC TGAGCCTCCT GTACCGCCTC CCAAACCTGA ATCTGTCCTG 
TCTCCAGGAG AACCGCCCCA TTCTGCAGCT TCCAAACCAT TGCCGCCTCC TCCCAGAGAT 
GATGACGATG ATGATGACGA TGATGATGAC CCTGCCATGA AGCCCCAGTC TGTGCTCAAC 
AGGGTCAAAA TGTTCGAAAA CAAACGCTCT GTGTCGGTAG ACCGAGCCAA GGACGCACCT 
GACGTCACTG GAATTAGGCC CACAGATCTC CCCAAACCTG CGAGCACCCC TGGACCTGTG 
CTCAAAGCCA ACTCTCTCAG CAACCTGGAG CAGGACAAAC CCTCCTACAG AGCTCCAGAG 
CCGCAGAAAC CTCAGACGCG TGTTGGGGAC GATGTGGTTC GCTCCAACCA CTACGACCCT 
GATGATGATG AAGAGTACTA CAGAAAACAG CTCTCATTTT TCGACCGCCG CAGTTTCGAC 
AGTAAGGTCA TGAATCCACC CAGCGCTGGA GTAAACCGCT TCCATGAGCC GCCCAAACCA 
CCCCAGACAC AGATCGCTTA TCCTTTCGCT AGGACGGAGT CTGTGGAGAA GGTGAGTCCT 
GTGGACAAGA GATACGAGCC TCTGCCGCCA GTCAACCCTT CACCTGCTCC ATACAGCCAG 
AACACACCTG TCGCTCCGCC CACATCCCTG CCCAAACTCA CCAACATCGA GGATCCTCAC 
AGCTCTCCCA AAGCCAAACC GGACCTGTCT GCTCTGAGAG CTCCGACCCG GGACGAGCCC 
ATCCAGAGCA GCTACTTGCC CCCCAAGTCC ACCATCAATG GCATGGATGC TCCTCCCAAA 
ACACTCGGCG TGCCGACCAG CTACAACCGC TACGTCCCCA AGCCTTACAC CAGCTCGGCG 
CGACCCTTCG AGCGCAAGTT CGAGAGTCCG AAATTCAACC ACAACCTGCT TCCCAACGAC 
ACGCAGTCCA AACCCAGTGT CAACAACAAC CTGAAGCCTC AGATCTCCCC ACAACCCCAG 
GACACGGACA GCGGCGTCGA CACCTTCACT CGCACCATGG ACAACAGGCC AAAATATCAG 
CACAACAACA TCAACGCCAT TCCCAAAGCC GTCCCAGTCA GCCCCAGTGC ACTGGATGAT 
GATGAAGAAG ATGAGGGACA CACAGTTGTG GCCACGGCGC GGGGCATCTT TAACTGTAAT 
GGCGGGGTGT TGAGCTCTAT AGAGACGGGC GTCAGCATCA TTATCCCACA GGGAGCCATT 
CCTGATAGTG TGGAGCAGGA GATCTACTTC AAAGTGTGCC GGGACAACAG CATCCTGCCT 
CCTCTGGATA AGGAGAAAGG TGAAACTCTG CTGAGTCCGC TGGTGATGTG TGGTCCTCAC 
GGCCTGAAGT TCCTGAAGCC TGTGGAGCTG CGCTTGCCAC ACTGTGCGTC TATGACCCCT 
GATGGTTGGT CTTTTGCTCT AAAATCCTCC GACTCCTCGT CGGGTGACCC CAAATGCTGG 
CAAAACAAGT GTCTACCAGG AGACCCCAAT TACCTGGTGG GCGCAAACTG TGTGTCTGTG 
CTGATCGACC ACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp1b ( XM_017354170.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017209659.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X12, mRNA.

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CloneID	ODa57881
Clone ID Related Accession (Same CDS sequence)	XM_017354170.2 , XM_017354170.1
Accession Version	XM_017354170.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6057bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tight junction protein ZO-1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017354170.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
TCCCGCAAGA ATGAAGCAGA ATACGGGCTT CGCCTGGCCA GCCACATCTT CGTGAAGGAC 
ATTTCCCCCG AGAGCCTGGC AGCCCGAGAC GGAAACATCC AAGAGGGAGA CGTGGTGCTT 
AAGATTAATG GCACGGTAAC AGAGAATTTG TCCCTGATCG ATGCTAAGAA GCTGATCGAG 
AGGTCAAAGG GAAAGCTGAA GATGGTGGTG CAGAGAGACG AGCGGGCGAC GCTGCTGAAC 
ATCCCCGATC TGGACGACAG CATACCTTCT GCTAATGCCT CGGACAGAGA TGATATTTCA 
GACATCCACT CCGTGGCCTC TGATCATTCC AACCGATCCC ACGACCGGAA GAGGAGCAGC 
CGCTCTCGTT CTCCAGACCG CCGCTCCGAA CCCTCAGACC ACTCCAGACA CTCTCCACCA 
CAGATCAGCA ACGGCAGTCA CAGGAGCCGT GATGAAGACA GGATCTCCAA GGCCGGGCCG 
CTTCCTCTAC CTGCTAAGAT GGTGGAGGAG ATGCCGAAAG AGCAGAGCGC AGCCAGAGAA 
ACAGAGAAAC AGCTCCCCCC TCTGCCAGAG CCCAAACCTG TGTACGCTCA GCCGGGCCAG 
CCGGACGTGG ACCTGCCTGT TAGTCCGGCA GATGCTCCTG TGCCCAGCGT CACACATGAT 
GACAGCATCT TACGGCCGAG CATGAAGCTG GTGAAGTTCA AGAAGGGCGA GAGCGTGGGC 
TTGAGGCTCG CTGGGGGGAA CGACGTTGGG ATATTTGTGG CGGGAGTTCT GGAGGACAGT 
CCTGCTGCTA AAGAGGGCCT GGAAGAGGGA GACCAGATTC TCAGGGTAAA TAATGTGGAT 
TTTGCAAATA TCATCCGAGA GGAGGCAGTG CTGTTCCTGC TGGATCTGCC CAAAGGAGAG 
GAGGTCACCA TCCTGGCCCA GAAGAAGAAA GACGTGTACC GCCGCATTGT AGAGTCTGAC 
GTGGGAGACT CGTTCTACAT CAGGACTCAT TTTGAATATG AGAAGGAGTC GCCCTATGGC 
CTGAGCTTCA ATAAGGGGGA GGTCTTCAGA GTCGTCGACA CCCTTTACAA TGGCAAACTG 
GGCTCCTGGC TCGCCATCCG CATCGGCAAA AACCACCAGG AGGTGGAGCG GGGCATCATC 
CCCAACAAGA ACAGGGCTGA GCAGTTATCA AGTGTCCAGT ACACTCTCCC AAAGACTCCA 
GGAGGGGATC GCGCCGACTT CTGGAGATTC AGAGGCCTCC GCAGCTCCAA ACGAAACCTG 
AGGAAGAGCA GAGAAGACCT GTCTGCTCAG CCGGTCCAAA CCAAATTCCC TGCTTATGAA 
AGAGTGGTGC TTAGAGAAGC TGGTTTCTTG AGGCCAGTGG TCATTTTCGG ACCGATCGCG 
GATGTGGCTA GAGAGAAGCT GTCCAGAGAG GAGACTGACC TCTTCGAACT TGCAAAGAGT 
GAACCCAGAG ATGCGGGCAC AGACCAGCGC AGCTCCGGAA TCATCCGTCT CCATACCATT 
AAACAGATCA TTGACCGAGA CAAGCACGCG GTGCTGGACA TCACCCCTAA TGCGGTGGAC 
CGTCTGAACT ACGCTCAGTG GTACCCCATC GTGGTGTTTC TGAATCCAGA CAGCAAACAG 
GGCGTCAAGA ACATGAGGAC CAGGCTCTGT CCCGAGTCCA GGAAGAGCGC CCGAAAGCTC 
TACGAGCGTG CTCTCAAACT GAGGAAGAAC AACCATCACC TTTTCACCAC CACTATTAAC 
TTGAACAACA TGAATGACGG GTGGTACGGT GCTTTGAAAG AGAGCATCCA GCAGCAGCAG 
AACCAGTTGG TCTGGGTCTC AGAGGGCAAG GCCGACGGCG CTCCTGACGA TGACCTTGAC 
ATCCATGACG ACCGCCTCTC CTACCTCTCG GCTCCGGGCA GCGAGTACAG CATGTACAGC 
ACCGACAGCC GCCACACGTC CGACTATGAG GACACCGACA CGGAGGGTGG TGCCTACACC 
GACCAGGAGC TGGACGAGAC CATGAACGAT GAGGTGGGCC TGCCTAGCGA GCCTGCCATC 
ACACGCTCAT CTGAGCCTGT GCGAGAGGAC CCGCCAGTCA TTCAGGACGC AGCTGGATAC 
CCCAGCTACC AGCATGCGGT ACCGCAACCT GAAGCGGTGA GCCGCATTGA CCCGGCCGGG 
TTTAAGATGG CAGCGCCGCA GCAGCAAGCT GAGGCTGCTC TGGCCTCGCC GCCCCCTCCC 
TCTGCAGCGG TCCAGCCCAC CGCAACACTA GCGGGTACGA ACCCCGAGGA GCCGCCTGGC 
ACCCCTCAGG CCGACTCCCT TAACAGCCCC GTCCCTGTTC CCGACCCCCA GCCCGAACCT 
GAGCTCGCTC AGCCCCCAAC ACACGACCCC CACCAGTCGC TTCCTCCTGG CCCAGATCCA 
AAGATGTATA AAAAGGATCT GTACAGCATG GACGAGCCGG TGCGAGTGAC CCACGGCGTG 
AAGCCTCCCC CTCCCCAGCA GCAGCAGCAG CCTTTAGGCC CCCCAACCTC GCTCTCCTAC 
AGCCACCAGC CTGTCTACCA GGACCAACAG CCATACCGCC AGTACGACCA CCCGCCTTAT 
GGCTATGACG GTGGCGGCTA CGCACAACCA AAGCCTCACA ACTACGACCC GCACCTGCAC 
TACGACAACC GTGTGCCTCA CTATAACGAG CAGTGGCCCA CGTACGACCA GCAGCAGCAG 
CAGCAGACTT CCCCCCAGCC GCCACCTGCG TCCGGCTTCC CTCAGGGCCA CCAGCCTCCG 
CCTCCACCAC CGATGGGATA TGAGCCACGC TCCCCATACG AGGACGGACC TAACAGGGAT 
TTCAGCCCTC CTCAGTCCCA GTATGACGGT GTGTCCCCAA TGGGCTACGA TCACCGGCCG 
CGGCACCCCA AACCTGCACC GGTCCGATAT GAAGAGCCTC CACCTCCACC TCCTCCGGTG 
TCCTACGATG CACGGTCACC TTTCGAACCC GAGCCGCATG CTTTCCCAGG CAACACACAT 
CGCTCGCCAG ACCCACCGAA ACAGTATTAT GGAGACGCCG CTATGAGGCC CTTGTACAAC 
CCTGGACCTC CGAACAGAGT GTACAAACAT GAGCCGGTGA ACTCTGAGCC TCCTGTACCG 
CCTCCCAAAC CTGAATCTGT CCTGTCTCCA GGAGAACCGC CCCATTCTGC AGCTTCCAAA 
CCATTGCCGC CTCCTCCCAG AGATGATGAC GATGATGATG ACGATGATGA TGACCCTGCC 
ATGAAGCCCC AGTCTGTGCT CAACAGGGTC AAAATGTTCG AAAACAAACG CTCTGTGTCG 
GTAGACCGAG CCAAGGACGC ACCTGACGTC ACTGGAATTA GGCCCACAGA TCTCCCCAAA 
CCTGCGAGCA CCCCTGGACC TGTGCTCAAA GCCAACTCTC TCAGCAACCT GGAGCAGGAC 
AAACCCTCCT ACAGAGCTCC AGAGCCGCAG AAACCTCAGA CGCGTGTTGG GGACGATGTG 
GTTCGCTCCA ACCACTACGA CCCTGATGAT GATGAAGAGT ACTACAGAAA ACAGCTCTCA 
TTTTTCGACC GCCGCAGTTT CGACAGTAAG GTCATGAATC CACCCAGCGC TGGAGTAAAC 
CGCTTCCATG AGCCGCCCAA ACCACCCCAG ACACAGATCG CTTATCCTTT CGCTAGGACG 
GAGTCTGTGG AGAAGGTGAG TCCTGTGGAC AAGAGATACG AGCCTCTGCC GCCAGTCAAC 
CCTTCACCTG CTCCATACAG CCAGAACACA CCTGTCGCTC CGCCCACATC CCTGCCCAAA 
CTCACCAACA TCGAGGTGAA CTCTCTGCCA GATCCTCACA GCTCTCCCAA AGCCAAACCG 
GACCTGTCTG CTCTGAGAGC TCCGACCCGG GACGAGCCCA TCCAGAGCAG CTACTTGCCC 
CCCAAGTCCA CCATCAATGG CATGGATGCT CCTCCCAAAA CACTCGGCGT GCCGACCAGC 
TACAACCGCT ACGTCCCCAA GCCTTACACC AGCTCGGCGC GACCCTTCGA GCGCAAGTTC 
GAGAGTCCGA AATTCAACCA CAACCTGCTT CCCAACGACA CGCAGTCCAA ACCCAGTGTC 
AACAACAACC TGAAGCCTCA GATCTCCCCA CAACCCCAGG ACACGGACAG CGGCGTCGAC 
ACCTTCACTC GCACCATGGA CAACAGGCCA AAATATCAGC ACAACAACAT CAACGCCATT 
CCCAAAGCCG TCCCAGTCAG CCCCAGTGCA CTGGATGATG ATGAAGAAGA TGAGGGACAC 
ACAGTTGTGG CCACGGCGCG GGGCATCTTT AACTGTAATG GCGGGGTGTT GAGCTCTATA 
GAGACGGGCG TCAGCATCAT TATCCCACAG GGAGCCATTC CTGATAGTGT GGAGCAGGAG 
ATCTACTTCA AAGTGTGCCG GGACAACAGC ATCCTGCCTC CTCTGGATAA GGAGAAAGGT 
GAAACTCTGC TGAGTCCGCT GGTGATGTGT GGTCCTCACG GCCTGAAGTT CCTGAAGCCT 
GTGGAGCTGC GCTTGCCACA CTGTGCGTCT ATGACCCCTG ATGGTTGGTC TTTTGCTCTA 
AAATCCTCCG ACTCCTCGTC GGGTGACCCC AAATGCTGGC AAAACAAGTG TCTACCAGGA 
GACCCCAATT ACCTGGTGGG CGCAAACTGT GTGTCTGTGC TGATCGACCA CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209659.1
CDS	110..6166
Translation	MLLSVFGGPVEEVLAGLLKLKMFGSGSKKWFIKKNKRQRTKVQRKPASGRRNKRVSRVLKRHKEVIVKQLDVSKVLPTLVYERVFTLGEYKEILGQDSSGKRAELFLDLLSQKEPAAFSAFCGVLEEVCPHLLTCFLLDGTDGSAGENKKQGQTKPAHSQQNALSKGQDTVCFPPMYTDPLFDTRVFPKHDKDKYGPAVYYPKFNAYIPNPDKVSVLDESEGSGHGKPSLRRIKGRIHRSKSLDSIDLLDSNSAAMDETVIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGLLQENDRVVMVNAVSMDNVDHAYAVQQLRKSGKNAKITIRRKRKVQIPSGRPGERETMSEHDEDDDSYEDEIYESRSARSGPSAYSGGGGGGGGGGASGRRSGRGDRLSGRRERDRERSGSRERSLSPRSDRHSVSSNLPPRPAKVTLVKSRKNEAEYGLRLASHIFVKDISPESLAARDGNIQEGDVVLKINGTVTENLSLIDAKKLIERSKGKLKMVVQRDERATLLNIPDLDDSIPSANASDRDDISDIHSVASDHSNRSHDRKRSSRSRSPDRRSEPSDHSRHSPPQISNGSHRSRDEDRISKAGPLPLPAKMVEEMPKEQSAARETEKQLPPLPEPKPVYAQPGQPDVDLPVSPADAPVPSVTHDDSILRPSMKLVKFKKGESVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFANIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHQEVERGIIPNKNRAEQLSSVQYTLPKTPGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVVIFGPIADVAREKLSREETDLFELAKSEPRDAGTDQRSSGIIRLHTIKQIIDRDKHAVLDITPNAVDRLNYAQWYPIVVFLNPDSKQGVKNMRTRLCPESRKSARKLYERALKLRKNNHHLFTTTINLNNMNDGWYGALKESIQQQQNQLVWVSEGKADGAPDDDLDIHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETMNDEVGLPSEPAITRSSEPVREDPPVIQDAAGYPSYQHAVPQPEAVSRIDPAGFKMAAPQQQAEAALASPPPPSAAVQPTATLAGTNPEEPPGTPQADSLNSPVPVPDPQPEPELAQPPTHDPHQSLPPGPDPKMYKKDLYSMDEPVRVTHGVKPPPPQQQQQPLGPPTSLSYSHQPVYQDQQPYRQYDHPPYGYDGGGYAQPKPHNYDPHLHYDNRVPHYNEQWPTYDQQQQQQTSPQPPPASGFPQGHQPPPPPPMGYEPRSPYEDGPNRDFSPPQSQYDGVSPMGYDHRPRHPKPAPVRYEEPPPPPPPVSYDARSPFEPEPHAFPGNTHRSPDPPKQYYGDAAMRPLYNPGPPNRVYKHEPVNSEPPVPPPKPESVLSPGEPPHSAASKPLPPPPRDDDDDDDDDDDPAMKPQSVLNRVKMFENKRSVSVDRAKDAPDVTGIRPTDLPKPASTPGPVLKANSLSNLEQDKPSYRAPEPQKPQTRVGDDVVRSNHYDPDDDEEYYRKQLSFFDRRSFDSKVMNPPSAGVNRFHEPPKPPQTQIAYPFARTESVEKVSPVDKRYEPLPPVNPSPAPYSQNTPVAPPTSLPKLTNIEVNSLPDPHSSPKAKPDLSALRAPTRDEPIQSSYLPPKSTINGMDAPPKTLGVPTSYNRYVPKPYTSSARPFERKFESPKFNHNLLPNDTQSKPSVNNNLKPQISPQPQDTDSGVDTFTRTMDNRPKYQHNNINAIPKAVPVSPSALDDDEEDEGHTVVATARGIFNCNGGVLSSIETGVSIIIPQGAIPDSVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKCWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_017354170.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tight junction protein 1b (tjp1b), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017354170.2

ORF Insert Sequence:

ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
TCCCGCAAGA ATGAAGCAGA ATACGGGCTT CGCCTGGCCA GCCACATCTT CGTGAAGGAC 
ATTTCCCCCG AGAGCCTGGC AGCCCGAGAC GGAAACATCC AAGAGGGAGA CGTGGTGCTT 
AAGATTAATG GCACGGTAAC AGAGAATTTG TCCCTGATCG ATGCTAAGAA GCTGATCGAG 
AGGTCAAAGG GAAAGCTGAA GATGGTGGTG CAGAGAGACG AGCGGGCGAC GCTGCTGAAC 
ATCCCCGATC TGGACGACAG CATACCTTCT GCTAATGCCT CGGACAGAGA TGATATTTCA 
GACATCCACT CCGTGGCCTC TGATCATTCC AACCGATCCC ACGACCGGAA GAGGAGCAGC 
CGCTCTCGTT CTCCAGACCG CCGCTCCGAA CCCTCAGACC ACTCCAGACA CTCTCCACCA 
CAGATCAGCA ACGGCAGTCA CAGGAGCCGT GATGAAGACA GGATCTCCAA GGCCGGGCCG 
CTTCCTCTAC CTGCTAAGAT GGTGGAGGAG ATGCCGAAAG AGCAGAGCGC AGCCAGAGAA 
ACAGAGAAAC AGCTCCCCCC TCTGCCAGAG CCCAAACCTG TGTACGCTCA GCCGGGCCAG 
CCGGACGTGG ACCTGCCTGT TAGTCCGGCA GATGCTCCTG TGCCCAGCGT CACACATGAT 
GACAGCATCT TACGGCCGAG CATGAAGCTG GTGAAGTTCA AGAAGGGCGA GAGCGTGGGC 
TTGAGGCTCG CTGGGGGGAA CGACGTTGGG ATATTTGTGG CGGGAGTTCT GGAGGACAGT 
CCTGCTGCTA AAGAGGGCCT GGAAGAGGGA GACCAGATTC TCAGGGTAAA TAATGTGGAT 
TTTGCAAATA TCATCCGAGA GGAGGCAGTG CTGTTCCTGC TGGATCTGCC CAAAGGAGAG 
GAGGTCACCA TCCTGGCCCA GAAGAAGAAA GACGTGTACC GCCGCATTGT AGAGTCTGAC 
GTGGGAGACT CGTTCTACAT CAGGACTCAT TTTGAATATG AGAAGGAGTC GCCCTATGGC 
CTGAGCTTCA ATAAGGGGGA GGTCTTCAGA GTCGTCGACA CCCTTTACAA TGGCAAACTG 
GGCTCCTGGC TCGCCATCCG CATCGGCAAA AACCACCAGG AGGTGGAGCG GGGCATCATC 
CCCAACAAGA ACAGGGCTGA GCAGTTATCA AGTGTCCAGT ACACTCTCCC AAAGACTCCA 
GGAGGGGATC GCGCCGACTT CTGGAGATTC AGAGGCCTCC GCAGCTCCAA ACGAAACCTG 
AGGAAGAGCA GAGAAGACCT GTCTGCTCAG CCGGTCCAAA CCAAATTCCC TGCTTATGAA 
AGAGTGGTGC TTAGAGAAGC TGGTTTCTTG AGGCCAGTGG TCATTTTCGG ACCGATCGCG 
GATGTGGCTA GAGAGAAGCT GTCCAGAGAG GAGACTGACC TCTTCGAACT TGCAAAGAGT 
GAACCCAGAG ATGCGGGCAC AGACCAGCGC AGCTCCGGAA TCATCCGTCT CCATACCATT 
AAACAGATCA TTGACCGAGA CAAGCACGCG GTGCTGGACA TCACCCCTAA TGCGGTGGAC 
CGTCTGAACT ACGCTCAGTG GTACCCCATC GTGGTGTTTC TGAATCCAGA CAGCAAACAG 
GGCGTCAAGA ACATGAGGAC CAGGCTCTGT CCCGAGTCCA GGAAGAGCGC CCGAAAGCTC 
TACGAGCGTG CTCTCAAACT GAGGAAGAAC AACCATCACC TTTTCACCAC CACTATTAAC 
TTGAACAACA TGAATGACGG GTGGTACGGT GCTTTGAAAG AGAGCATCCA GCAGCAGCAG 
AACCAGTTGG TCTGGGTCTC AGAGGGCAAG GCCGACGGCG CTCCTGACGA TGACCTTGAC 
ATCCATGACG ACCGCCTCTC CTACCTCTCG GCTCCGGGCA GCGAGTACAG CATGTACAGC 
ACCGACAGCC GCCACACGTC CGACTATGAG GACACCGACA CGGAGGGTGG TGCCTACACC 
GACCAGGAGC TGGACGAGAC CATGAACGAT GAGGTGGGCC TGCCTAGCGA GCCTGCCATC 
ACACGCTCAT CTGAGCCTGT GCGAGAGGAC CCGCCAGTCA TTCAGGACGC AGCTGGATAC 
CCCAGCTACC AGCATGCGGT ACCGCAACCT GAAGCGGTGA GCCGCATTGA CCCGGCCGGG 
TTTAAGATGG CAGCGCCGCA GCAGCAAGCT GAGGCTGCTC TGGCCTCGCC GCCCCCTCCC 
TCTGCAGCGG TCCAGCCCAC CGCAACACTA GCGGGTACGA ACCCCGAGGA GCCGCCTGGC 
ACCCCTCAGG CCGACTCCCT TAACAGCCCC GTCCCTGTTC CCGACCCCCA GCCCGAACCT 
GAGCTCGCTC AGCCCCCAAC ACACGACCCC CACCAGTCGC TTCCTCCTGG CCCAGATCCA 
AAGATGTATA AAAAGGATCT GTACAGCATG GACGAGCCGG TGCGAGTGAC CCACGGCGTG 
AAGCCTCCCC CTCCCCAGCA GCAGCAGCAG CCTTTAGGCC CCCCAACCTC GCTCTCCTAC 
AGCCACCAGC CTGTCTACCA GGACCAACAG CCATACCGCC AGTACGACCA CCCGCCTTAT 
GGCTATGACG GTGGCGGCTA CGCACAACCA AAGCCTCACA ACTACGACCC GCACCTGCAC 
TACGACAACC GTGTGCCTCA CTATAACGAG CAGTGGCCCA CGTACGACCA GCAGCAGCAG 
CAGCAGACTT CCCCCCAGCC GCCACCTGCG TCCGGCTTCC CTCAGGGCCA CCAGCCTCCG 
CCTCCACCAC CGATGGGATA TGAGCCACGC TCCCCATACG AGGACGGACC TAACAGGGAT 
TTCAGCCCTC CTCAGTCCCA GTATGACGGT GTGTCCCCAA TGGGCTACGA TCACCGGCCG 
CGGCACCCCA AACCTGCACC GGTCCGATAT GAAGAGCCTC CACCTCCACC TCCTCCGGTG 
TCCTACGATG CACGGTCACC TTTCGAACCC GAGCCGCATG CTTTCCCAGG CAACACACAT 
CGCTCGCCAG ACCCACCGAA ACAGTATTAT GGAGACGCCG CTATGAGGCC CTTGTACAAC 
CCTGGACCTC CGAACAGAGT GTACAAACAT GAGCCGGTGA ACTCTGAGCC TCCTGTACCG 
CCTCCCAAAC CTGAATCTGT CCTGTCTCCA GGAGAACCGC CCCATTCTGC AGCTTCCAAA 
CCATTGCCGC CTCCTCCCAG AGATGATGAC GATGATGATG ACGATGATGA TGACCCTGCC 
ATGAAGCCCC AGTCTGTGCT CAACAGGGTC AAAATGTTCG AAAACAAACG CTCTGTGTCG 
GTAGACCGAG CCAAGGACGC ACCTGACGTC ACTGGAATTA GGCCCACAGA TCTCCCCAAA 
CCTGCGAGCA CCCCTGGACC TGTGCTCAAA GCCAACTCTC TCAGCAACCT GGAGCAGGAC 
AAACCCTCCT ACAGAGCTCC AGAGCCGCAG AAACCTCAGA CGCGTGTTGG GGACGATGTG 
GTTCGCTCCA ACCACTACGA CCCTGATGAT GATGAAGAGT ACTACAGAAA ACAGCTCTCA 
TTTTTCGACC GCCGCAGTTT CGACAGTAAG GTCATGAATC CACCCAGCGC TGGAGTAAAC 
CGCTTCCATG AGCCGCCCAA ACCACCCCAG ACACAGATCG CTTATCCTTT CGCTAGGACG 
GAGTCTGTGG AGAAGGTGAG TCCTGTGGAC AAGAGATACG AGCCTCTGCC GCCAGTCAAC 
CCTTCACCTG CTCCATACAG CCAGAACACA CCTGTCGCTC CGCCCACATC CCTGCCCAAA 
CTCACCAACA TCGAGGTGAA CTCTCTGCCA GATCCTCACA GCTCTCCCAA AGCCAAACCG 
GACCTGTCTG CTCTGAGAGC TCCGACCCGG GACGAGCCCA TCCAGAGCAG CTACTTGCCC 
CCCAAGTCCA CCATCAATGG CATGGATGCT CCTCCCAAAA CACTCGGCGT GCCGACCAGC 
TACAACCGCT ACGTCCCCAA GCCTTACACC AGCTCGGCGC GACCCTTCGA GCGCAAGTTC 
GAGAGTCCGA AATTCAACCA CAACCTGCTT CCCAACGACA CGCAGTCCAA ACCCAGTGTC 
AACAACAACC TGAAGCCTCA GATCTCCCCA CAACCCCAGG ACACGGACAG CGGCGTCGAC 
ACCTTCACTC GCACCATGGA CAACAGGCCA AAATATCAGC ACAACAACAT CAACGCCATT 
CCCAAAGCCG TCCCAGTCAG CCCCAGTGCA CTGGATGATG ATGAAGAAGA TGAGGGACAC 
ACAGTTGTGG CCACGGCGCG GGGCATCTTT AACTGTAATG GCGGGGTGTT GAGCTCTATA 
GAGACGGGCG TCAGCATCAT TATCCCACAG GGAGCCATTC CTGATAGTGT GGAGCAGGAG 
ATCTACTTCA AAGTGTGCCG GGACAACAGC ATCCTGCCTC CTCTGGATAA GGAGAAAGGT 
GAAACTCTGC TGAGTCCGCT GGTGATGTGT GGTCCTCACG GCCTGAAGTT CCTGAAGCCT 
GTGGAGCTGC GCTTGCCACA CTGTGCGTCT ATGACCCCTG ATGGTTGGTC TTTTGCTCTA 
AAATCCTCCG ACTCCTCGTC GGGTGACCCC AAATGCTGGC AAAACAAGTG TCTACCAGGA 
GACCCCAATT ACCTGGTGGG CGCAAACTGT GTGTCTGTGC TGATCGACCA CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp1b ( XM_017354170.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017209659.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X5, mRNA.

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CloneID	ODa57881
Clone ID Related Accession (Same CDS sequence)	XM_017354170.2 , XM_017354170.1
Accession Version	XM_017354170.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6057bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tight junction protein ZO-1 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
TCCCGCAAGA ATGAAGCAGA ATACGGGCTT CGCCTGGCCA GCCACATCTT CGTGAAGGAC 
ATTTCCCCCG AGAGCCTGGC AGCCCGAGAC GGAAACATCC AAGAGGGAGA CGTGGTGCTT 
AAGATTAATG GCACGGTAAC AGAGAATTTG TCCCTGATCG ATGCTAAGAA GCTGATCGAG 
AGGTCAAAGG GAAAGCTGAA GATGGTGGTG CAGAGAGACG AGCGGGCGAC GCTGCTGAAC 
ATCCCCGATC TGGACGACAG CATACCTTCT GCTAATGCCT CGGACAGAGA TGATATTTCA 
GACATCCACT CCGTGGCCTC TGATCATTCC AACCGATCCC ACGACCGGAA GAGGAGCAGC 
CGCTCTCGTT CTCCAGACCG CCGCTCCGAA CCCTCAGACC ACTCCAGACA CTCTCCACCA 
CAGATCAGCA ACGGCAGTCA CAGGAGCCGT GATGAAGACA GGATCTCCAA GGCCGGGCCG 
CTTCCTCTAC CTGCTAAGAT GGTGGAGGAG ATGCCGAAAG AGCAGAGCGC AGCCAGAGAA 
ACAGAGAAAC AGCTCCCCCC TCTGCCAGAG CCCAAACCTG TGTACGCTCA GCCGGGCCAG 
CCGGACGTGG ACCTGCCTGT TAGTCCGGCA GATGCTCCTG TGCCCAGCGT CACACATGAT 
GACAGCATCT TACGGCCGAG CATGAAGCTG GTGAAGTTCA AGAAGGGCGA GAGCGTGGGC 
TTGAGGCTCG CTGGGGGGAA CGACGTTGGG ATATTTGTGG CGGGAGTTCT GGAGGACAGT 
CCTGCTGCTA AAGAGGGCCT GGAAGAGGGA GACCAGATTC TCAGGGTAAA TAATGTGGAT 
TTTGCAAATA TCATCCGAGA GGAGGCAGTG CTGTTCCTGC TGGATCTGCC CAAAGGAGAG 
GAGGTCACCA TCCTGGCCCA GAAGAAGAAA GACGTGTACC GCCGCATTGT AGAGTCTGAC 
GTGGGAGACT CGTTCTACAT CAGGACTCAT TTTGAATATG AGAAGGAGTC GCCCTATGGC 
CTGAGCTTCA ATAAGGGGGA GGTCTTCAGA GTCGTCGACA CCCTTTACAA TGGCAAACTG 
GGCTCCTGGC TCGCCATCCG CATCGGCAAA AACCACCAGG AGGTGGAGCG GGGCATCATC 
CCCAACAAGA ACAGGGCTGA GCAGTTATCA AGTGTCCAGT ACACTCTCCC AAAGACTCCA 
GGAGGGGATC GCGCCGACTT CTGGAGATTC AGAGGCCTCC GCAGCTCCAA ACGAAACCTG 
AGGAAGAGCA GAGAAGACCT GTCTGCTCAG CCGGTCCAAA CCAAATTCCC TGCTTATGAA 
AGAGTGGTGC TTAGAGAAGC TGGTTTCTTG AGGCCAGTGG TCATTTTCGG ACCGATCGCG 
GATGTGGCTA GAGAGAAGCT GTCCAGAGAG GAGACTGACC TCTTCGAACT TGCAAAGAGT 
GAACCCAGAG ATGCGGGCAC AGACCAGCGC AGCTCCGGAA TCATCCGTCT CCATACCATT 
AAACAGATCA TTGACCGAGA CAAGCACGCG GTGCTGGACA TCACCCCTAA TGCGGTGGAC 
CGTCTGAACT ACGCTCAGTG GTACCCCATC GTGGTGTTTC TGAATCCAGA CAGCAAACAG 
GGCGTCAAGA ACATGAGGAC CAGGCTCTGT CCCGAGTCCA GGAAGAGCGC CCGAAAGCTC 
TACGAGCGTG CTCTCAAACT GAGGAAGAAC AACCATCACC TTTTCACCAC CACTATTAAC 
TTGAACAACA TGAATGACGG GTGGTACGGT GCTTTGAAAG AGAGCATCCA GCAGCAGCAG 
AACCAGTTGG TCTGGGTCTC AGAGGGCAAG GCCGACGGCG CTCCTGACGA TGACCTTGAC 
ATCCATGACG ACCGCCTCTC CTACCTCTCG GCTCCGGGCA GCGAGTACAG CATGTACAGC 
ACCGACAGCC GCCACACGTC CGACTATGAG GACACCGACA CGGAGGGTGG TGCCTACACC 
GACCAGGAGC TGGACGAGAC CATGAACGAT GAGGTGGGCC TGCCTAGCGA GCCTGCCATC 
ACACGCTCAT CTGAGCCTGT GCGAGAGGAC CCGCCAGTCA TTCAGGACGC AGCTGGATAC 
CCCAGCTACC AGCATGCGGT ACCGCAACCT GAAGCGGTGA GCCGCATTGA CCCGGCCGGG 
TTTAAGATGG CAGCGCCGCA GCAGCAAGCT GAGGCTGCTC TGGCCTCGCC GCCCCCTCCC 
TCTGCAGCGG TCCAGCCCAC CGCAACACTA GCGGGTACGA ACCCCGAGGA GCCGCCTGGC 
ACCCCTCAGG CCGACTCCCT TAACAGCCCC GTCCCTGTTC CCGACCCCCA GCCCGAACCT 
GAGCTCGCTC AGCCCCCAAC ACACGACCCC CACCAGTCGC TTCCTCCTGG CCCAGATCCA 
AAGATGTATA AAAAGGATCT GTACAGCATG GACGAGCCGG TGCGAGTGAC CCACGGCGTG 
AAGCCTCCCC CTCCCCAGCA GCAGCAGCAG CCTTTAGGCC CCCCAACCTC GCTCTCCTAC 
AGCCACCAGC CTGTCTACCA GGACCAACAG CCATACCGCC AGTACGACCA CCCGCCTTAT 
GGCTATGACG GTGGCGGCTA CGCACAACCA AAGCCTCACA ACTACGACCC GCACCTGCAC 
TACGACAACC GTGTGCCTCA CTATAACGAG CAGTGGCCCA CGTACGACCA GCAGCAGCAG 
CAGCAGACTT CCCCCCAGCC GCCACCTGCG TCCGGCTTCC CTCAGGGCCA CCAGCCTCCG 
CCTCCACCAC CGATGGGATA TGAGCCACGC TCCCCATACG AGGACGGACC TAACAGGGAT 
TTCAGCCCTC CTCAGTCCCA GTATGACGGT GTGTCCCCAA TGGGCTACGA TCACCGGCCG 
CGGCACCCCA AACCTGCACC GGTCCGATAT GAAGAGCCTC CACCTCCACC TCCTCCGGTG 
TCCTACGATG CACGGTCACC TTTCGAACCC GAGCCGCATG CTTTCCCAGG CAACACACAT 
CGCTCGCCAG ACCCACCGAA ACAGTATTAT GGAGACGCCG CTATGAGGCC CTTGTACAAC 
CCTGGACCTC CGAACAGAGT GTACAAACAT GAGCCGGTGA ACTCTGAGCC TCCTGTACCG 
CCTCCCAAAC CTGAATCTGT CCTGTCTCCA GGAGAACCGC CCCATTCTGC AGCTTCCAAA 
CCATTGCCGC CTCCTCCCAG AGATGATGAC GATGATGATG ACGATGATGA TGACCCTGCC 
ATGAAGCCCC AGTCTGTGCT CAACAGGGTC AAAATGTTCG AAAACAAACG CTCTGTGTCG 
GTAGACCGAG CCAAGGACGC ACCTGACGTC ACTGGAATTA GGCCCACAGA TCTCCCCAAA 
CCTGCGAGCA CCCCTGGACC TGTGCTCAAA GCCAACTCTC TCAGCAACCT GGAGCAGGAC 
AAACCCTCCT ACAGAGCTCC AGAGCCGCAG AAACCTCAGA CGCGTGTTGG GGACGATGTG 
GTTCGCTCCA ACCACTACGA CCCTGATGAT GATGAAGAGT ACTACAGAAA ACAGCTCTCA 
TTTTTCGACC GCCGCAGTTT CGACAGTAAG GTCATGAATC CACCCAGCGC TGGAGTAAAC 
CGCTTCCATG AGCCGCCCAA ACCACCCCAG ACACAGATCG CTTATCCTTT CGCTAGGACG 
GAGTCTGTGG AGAAGGTGAG TCCTGTGGAC AAGAGATACG AGCCTCTGCC GCCAGTCAAC 
CCTTCACCTG CTCCATACAG CCAGAACACA CCTGTCGCTC CGCCCACATC CCTGCCCAAA 
CTCACCAACA TCGAGGTGAA CTCTCTGCCA GATCCTCACA GCTCTCCCAA AGCCAAACCG 
GACCTGTCTG CTCTGAGAGC TCCGACCCGG GACGAGCCCA TCCAGAGCAG CTACTTGCCC 
CCCAAGTCCA CCATCAATGG CATGGATGCT CCTCCCAAAA CACTCGGCGT GCCGACCAGC 
TACAACCGCT ACGTCCCCAA GCCTTACACC AGCTCGGCGC GACCCTTCGA GCGCAAGTTC 
GAGAGTCCGA AATTCAACCA CAACCTGCTT CCCAACGACA CGCAGTCCAA ACCCAGTGTC 
AACAACAACC TGAAGCCTCA GATCTCCCCA CAACCCCAGG ACACGGACAG CGGCGTCGAC 
ACCTTCACTC GCACCATGGA CAACAGGCCA AAATATCAGC ACAACAACAT CAACGCCATT 
CCCAAAGCCG TCCCAGTCAG CCCCAGTGCA CTGGATGATG ATGAAGAAGA TGAGGGACAC 
ACAGTTGTGG CCACGGCGCG GGGCATCTTT AACTGTAATG GCGGGGTGTT GAGCTCTATA 
GAGACGGGCG TCAGCATCAT TATCCCACAG GGAGCCATTC CTGATAGTGT GGAGCAGGAG 
ATCTACTTCA AAGTGTGCCG GGACAACAGC ATCCTGCCTC CTCTGGATAA GGAGAAAGGT 
GAAACTCTGC TGAGTCCGCT GGTGATGTGT GGTCCTCACG GCCTGAAGTT CCTGAAGCCT 
GTGGAGCTGC GCTTGCCACA CTGTGCGTCT ATGACCCCTG ATGGTTGGTC TTTTGCTCTA 
AAATCCTCCG ACTCCTCGTC GGGTGACCCC AAATGCTGGC AAAACAAGTG TCTACCAGGA 
GACCCCAATT ACCTGGTGGG CGCAAACTGT GTGTCTGTGC TGATCGACCA CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209659.1
CDS	115..6171
Translation	MLLSVFGGPVEEVLAGLLKLKMFGSGSKKWFIKKNKRQRTKVQRKPASGRRNKRVSRVLKRHKEVIVKQLDVSKVLPTLVYERVFTLGEYKEILGQDSSGKRAELFLDLLSQKEPAAFSAFCGVLEEVCPHLLTCFLLDGTDGSAGENKKQGQTKPAHSQQNALSKGQDTVCFPPMYTDPLFDTRVFPKHDKDKYGPAVYYPKFNAYIPNPDKVSVLDESEGSGHGKPSLRRIKGRIHRSKSLDSIDLLDSNSAAMDETVIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGLLQENDRVVMVNAVSMDNVDHAYAVQQLRKSGKNAKITIRRKRKVQIPSGRPGERETMSEHDEDDDSYEDEIYESRSARSGPSAYSGGGGGGGGGGASGRRSGRGDRLSGRRERDRERSGSRERSLSPRSDRHSVSSNLPPRPAKVTLVKSRKNEAEYGLRLASHIFVKDISPESLAARDGNIQEGDVVLKINGTVTENLSLIDAKKLIERSKGKLKMVVQRDERATLLNIPDLDDSIPSANASDRDDISDIHSVASDHSNRSHDRKRSSRSRSPDRRSEPSDHSRHSPPQISNGSHRSRDEDRISKAGPLPLPAKMVEEMPKEQSAARETEKQLPPLPEPKPVYAQPGQPDVDLPVSPADAPVPSVTHDDSILRPSMKLVKFKKGESVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFANIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHQEVERGIIPNKNRAEQLSSVQYTLPKTPGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVVIFGPIADVAREKLSREETDLFELAKSEPRDAGTDQRSSGIIRLHTIKQIIDRDKHAVLDITPNAVDRLNYAQWYPIVVFLNPDSKQGVKNMRTRLCPESRKSARKLYERALKLRKNNHHLFTTTINLNNMNDGWYGALKESIQQQQNQLVWVSEGKADGAPDDDLDIHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETMNDEVGLPSEPAITRSSEPVREDPPVIQDAAGYPSYQHAVPQPEAVSRIDPAGFKMAAPQQQAEAALASPPPPSAAVQPTATLAGTNPEEPPGTPQADSLNSPVPVPDPQPEPELAQPPTHDPHQSLPPGPDPKMYKKDLYSMDEPVRVTHGVKPPPPQQQQQPLGPPTSLSYSHQPVYQDQQPYRQYDHPPYGYDGGGYAQPKPHNYDPHLHYDNRVPHYNEQWPTYDQQQQQQTSPQPPPASGFPQGHQPPPPPPMGYEPRSPYEDGPNRDFSPPQSQYDGVSPMGYDHRPRHPKPAPVRYEEPPPPPPPVSYDARSPFEPEPHAFPGNTHRSPDPPKQYYGDAAMRPLYNPGPPNRVYKHEPVNSEPPVPPPKPESVLSPGEPPHSAASKPLPPPPRDDDDDDDDDDDPAMKPQSVLNRVKMFENKRSVSVDRAKDAPDVTGIRPTDLPKPASTPGPVLKANSLSNLEQDKPSYRAPEPQKPQTRVGDDVVRSNHYDPDDDEEYYRKQLSFFDRRSFDSKVMNPPSAGVNRFHEPPKPPQTQIAYPFARTESVEKVSPVDKRYEPLPPVNPSPAPYSQNTPVAPPTSLPKLTNIEVNSLPDPHSSPKAKPDLSALRAPTRDEPIQSSYLPPKSTINGMDAPPKTLGVPTSYNRYVPKPYTSSARPFERKFESPKFNHNLLPNDTQSKPSVNNNLKPQISPQPQDTDSGVDTFTRTMDNRPKYQHNNINAIPKAVPVSPSALDDDEEDEGHTVVATARGIFNCNGGVLSSIETGVSIIIPQGAIPDSVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKCWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_017354170.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tight junction protein 1b (tjp1b), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017354170.1

ORF Insert Sequence:

ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
TCCCGCAAGA ATGAAGCAGA ATACGGGCTT CGCCTGGCCA GCCACATCTT CGTGAAGGAC 
ATTTCCCCCG AGAGCCTGGC AGCCCGAGAC GGAAACATCC AAGAGGGAGA CGTGGTGCTT 
AAGATTAATG GCACGGTAAC AGAGAATTTG TCCCTGATCG ATGCTAAGAA GCTGATCGAG 
AGGTCAAAGG GAAAGCTGAA GATGGTGGTG CAGAGAGACG AGCGGGCGAC GCTGCTGAAC 
ATCCCCGATC TGGACGACAG CATACCTTCT GCTAATGCCT CGGACAGAGA TGATATTTCA 
GACATCCACT CCGTGGCCTC TGATCATTCC AACCGATCCC ACGACCGGAA GAGGAGCAGC 
CGCTCTCGTT CTCCAGACCG CCGCTCCGAA CCCTCAGACC ACTCCAGACA CTCTCCACCA 
CAGATCAGCA ACGGCAGTCA CAGGAGCCGT GATGAAGACA GGATCTCCAA GGCCGGGCCG 
CTTCCTCTAC CTGCTAAGAT GGTGGAGGAG ATGCCGAAAG AGCAGAGCGC AGCCAGAGAA 
ACAGAGAAAC AGCTCCCCCC TCTGCCAGAG CCCAAACCTG TGTACGCTCA GCCGGGCCAG 
CCGGACGTGG ACCTGCCTGT TAGTCCGGCA GATGCTCCTG TGCCCAGCGT CACACATGAT 
GACAGCATCT TACGGCCGAG CATGAAGCTG GTGAAGTTCA AGAAGGGCGA GAGCGTGGGC 
TTGAGGCTCG CTGGGGGGAA CGACGTTGGG ATATTTGTGG CGGGAGTTCT GGAGGACAGT 
CCTGCTGCTA AAGAGGGCCT GGAAGAGGGA GACCAGATTC TCAGGGTAAA TAATGTGGAT 
TTTGCAAATA TCATCCGAGA GGAGGCAGTG CTGTTCCTGC TGGATCTGCC CAAAGGAGAG 
GAGGTCACCA TCCTGGCCCA GAAGAAGAAA GACGTGTACC GCCGCATTGT AGAGTCTGAC 
GTGGGAGACT CGTTCTACAT CAGGACTCAT TTTGAATATG AGAAGGAGTC GCCCTATGGC 
CTGAGCTTCA ATAAGGGGGA GGTCTTCAGA GTCGTCGACA CCCTTTACAA TGGCAAACTG 
GGCTCCTGGC TCGCCATCCG CATCGGCAAA AACCACCAGG AGGTGGAGCG GGGCATCATC 
CCCAACAAGA ACAGGGCTGA GCAGTTATCA AGTGTCCAGT ACACTCTCCC AAAGACTCCA 
GGAGGGGATC GCGCCGACTT CTGGAGATTC AGAGGCCTCC GCAGCTCCAA ACGAAACCTG 
AGGAAGAGCA GAGAAGACCT GTCTGCTCAG CCGGTCCAAA CCAAATTCCC TGCTTATGAA 
AGAGTGGTGC TTAGAGAAGC TGGTTTCTTG AGGCCAGTGG TCATTTTCGG ACCGATCGCG 
GATGTGGCTA GAGAGAAGCT GTCCAGAGAG GAGACTGACC TCTTCGAACT TGCAAAGAGT 
GAACCCAGAG ATGCGGGCAC AGACCAGCGC AGCTCCGGAA TCATCCGTCT CCATACCATT 
AAACAGATCA TTGACCGAGA CAAGCACGCG GTGCTGGACA TCACCCCTAA TGCGGTGGAC 
CGTCTGAACT ACGCTCAGTG GTACCCCATC GTGGTGTTTC TGAATCCAGA CAGCAAACAG 
GGCGTCAAGA ACATGAGGAC CAGGCTCTGT CCCGAGTCCA GGAAGAGCGC CCGAAAGCTC 
TACGAGCGTG CTCTCAAACT GAGGAAGAAC AACCATCACC TTTTCACCAC CACTATTAAC 
TTGAACAACA TGAATGACGG GTGGTACGGT GCTTTGAAAG AGAGCATCCA GCAGCAGCAG 
AACCAGTTGG TCTGGGTCTC AGAGGGCAAG GCCGACGGCG CTCCTGACGA TGACCTTGAC 
ATCCATGACG ACCGCCTCTC CTACCTCTCG GCTCCGGGCA GCGAGTACAG CATGTACAGC 
ACCGACAGCC GCCACACGTC CGACTATGAG GACACCGACA CGGAGGGTGG TGCCTACACC 
GACCAGGAGC TGGACGAGAC CATGAACGAT GAGGTGGGCC TGCCTAGCGA GCCTGCCATC 
ACACGCTCAT CTGAGCCTGT GCGAGAGGAC CCGCCAGTCA TTCAGGACGC AGCTGGATAC 
CCCAGCTACC AGCATGCGGT ACCGCAACCT GAAGCGGTGA GCCGCATTGA CCCGGCCGGG 
TTTAAGATGG CAGCGCCGCA GCAGCAAGCT GAGGCTGCTC TGGCCTCGCC GCCCCCTCCC 
TCTGCAGCGG TCCAGCCCAC CGCAACACTA GCGGGTACGA ACCCCGAGGA GCCGCCTGGC 
ACCCCTCAGG CCGACTCCCT TAACAGCCCC GTCCCTGTTC CCGACCCCCA GCCCGAACCT 
GAGCTCGCTC AGCCCCCAAC ACACGACCCC CACCAGTCGC TTCCTCCTGG CCCAGATCCA 
AAGATGTATA AAAAGGATCT GTACAGCATG GACGAGCCGG TGCGAGTGAC CCACGGCGTG 
AAGCCTCCCC CTCCCCAGCA GCAGCAGCAG CCTTTAGGCC CCCCAACCTC GCTCTCCTAC 
AGCCACCAGC CTGTCTACCA GGACCAACAG CCATACCGCC AGTACGACCA CCCGCCTTAT 
GGCTATGACG GTGGCGGCTA CGCACAACCA AAGCCTCACA ACTACGACCC GCACCTGCAC 
TACGACAACC GTGTGCCTCA CTATAACGAG CAGTGGCCCA CGTACGACCA GCAGCAGCAG 
CAGCAGACTT CCCCCCAGCC GCCACCTGCG TCCGGCTTCC CTCAGGGCCA CCAGCCTCCG 
CCTCCACCAC CGATGGGATA TGAGCCACGC TCCCCATACG AGGACGGACC TAACAGGGAT 
TTCAGCCCTC CTCAGTCCCA GTATGACGGT GTGTCCCCAA TGGGCTACGA TCACCGGCCG 
CGGCACCCCA AACCTGCACC GGTCCGATAT GAAGAGCCTC CACCTCCACC TCCTCCGGTG 
TCCTACGATG CACGGTCACC TTTCGAACCC GAGCCGCATG CTTTCCCAGG CAACACACAT 
CGCTCGCCAG ACCCACCGAA ACAGTATTAT GGAGACGCCG CTATGAGGCC CTTGTACAAC 
CCTGGACCTC CGAACAGAGT GTACAAACAT GAGCCGGTGA ACTCTGAGCC TCCTGTACCG 
CCTCCCAAAC CTGAATCTGT CCTGTCTCCA GGAGAACCGC CCCATTCTGC AGCTTCCAAA 
CCATTGCCGC CTCCTCCCAG AGATGATGAC GATGATGATG ACGATGATGA TGACCCTGCC 
ATGAAGCCCC AGTCTGTGCT CAACAGGGTC AAAATGTTCG AAAACAAACG CTCTGTGTCG 
GTAGACCGAG CCAAGGACGC ACCTGACGTC ACTGGAATTA GGCCCACAGA TCTCCCCAAA 
CCTGCGAGCA CCCCTGGACC TGTGCTCAAA GCCAACTCTC TCAGCAACCT GGAGCAGGAC 
AAACCCTCCT ACAGAGCTCC AGAGCCGCAG AAACCTCAGA CGCGTGTTGG GGACGATGTG 
GTTCGCTCCA ACCACTACGA CCCTGATGAT GATGAAGAGT ACTACAGAAA ACAGCTCTCA 
TTTTTCGACC GCCGCAGTTT CGACAGTAAG GTCATGAATC CACCCAGCGC TGGAGTAAAC 
CGCTTCCATG AGCCGCCCAA ACCACCCCAG ACACAGATCG CTTATCCTTT CGCTAGGACG 
GAGTCTGTGG AGAAGGTGAG TCCTGTGGAC AAGAGATACG AGCCTCTGCC GCCAGTCAAC 
CCTTCACCTG CTCCATACAG CCAGAACACA CCTGTCGCTC CGCCCACATC CCTGCCCAAA 
CTCACCAACA TCGAGGTGAA CTCTCTGCCA GATCCTCACA GCTCTCCCAA AGCCAAACCG 
GACCTGTCTG CTCTGAGAGC TCCGACCCGG GACGAGCCCA TCCAGAGCAG CTACTTGCCC 
CCCAAGTCCA CCATCAATGG CATGGATGCT CCTCCCAAAA CACTCGGCGT GCCGACCAGC 
TACAACCGCT ACGTCCCCAA GCCTTACACC AGCTCGGCGC GACCCTTCGA GCGCAAGTTC 
GAGAGTCCGA AATTCAACCA CAACCTGCTT CCCAACGACA CGCAGTCCAA ACCCAGTGTC 
AACAACAACC TGAAGCCTCA GATCTCCCCA CAACCCCAGG ACACGGACAG CGGCGTCGAC 
ACCTTCACTC GCACCATGGA CAACAGGCCA AAATATCAGC ACAACAACAT CAACGCCATT 
CCCAAAGCCG TCCCAGTCAG CCCCAGTGCA CTGGATGATG ATGAAGAAGA TGAGGGACAC 
ACAGTTGTGG CCACGGCGCG GGGCATCTTT AACTGTAATG GCGGGGTGTT GAGCTCTATA 
GAGACGGGCG TCAGCATCAT TATCCCACAG GGAGCCATTC CTGATAGTGT GGAGCAGGAG 
ATCTACTTCA AAGTGTGCCG GGACAACAGC ATCCTGCCTC CTCTGGATAA GGAGAAAGGT 
GAAACTCTGC TGAGTCCGCT GGTGATGTGT GGTCCTCACG GCCTGAAGTT CCTGAAGCCT 
GTGGAGCTGC GCTTGCCACA CTGTGCGTCT ATGACCCCTG ATGGTTGGTC TTTTGCTCTA 
AAATCCTCCG ACTCCTCGTC GGGTGACCCC AAATGCTGGC AAAACAAGTG TCTACCAGGA 
GACCCCAATT ACCTGGTGGG CGCAAACTGT GTGTCTGTGC TGATCGACCA CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp1b ( XM_017354171.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017209660.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X7, mRNA.

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CloneID	ODa57882
Clone ID Related Accession (Same CDS sequence)	XM_017354171.1
Accession Version	XM_017354171.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tight junction protein ZO-1 isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
TCCCGCAAGA ATGAAGCAGA ATACGGGCTT CGCCTGGCCA GCCACATCTT CGTGAAGGAC 
ATTTCCCCCG AGAGCCTGGC AGCCCGAGAC GGAAACATCC AAGAGGGAGA CGTGGTGCTT 
AAGATTAATG GCACGGTAAC AGAGAATTTG TCCCTGATCG ATGCTAAGAA GCTGATCGAG 
AGGTCAAAGG GAAAGCTGAA GATGGTGGTG CAGAGAGACG AGCGGGCGAC GCTGCTGAAC 
ATCCCCGATC TGGACGACAG CATACCTTCT GCTAATGCCT CGGACAGAGA TGATATTTCA 
GACATCCACT CCGTGGCCTC TGATCATTCC AACCGATCCC ACGACCGGAA GAGGAGCAGC 
CGCTCTCGTT CTCCAGACCG CCGCTCCGAA CCCTCAGACC ACTCCAGACA CTCTCCACCA 
CAGATCAGCA ACGGCAGGGC CACAGGGCCG ACGGCTCAGA GAACGCGGCC AGTTCACAGG 
AGCCGTGATG AAGACAGGAT CTCCAAGGCC GGGCCGCTTC CTCTACCTGC TAAGATGGTG 
GAGGAGATGC CGAAAGAGCA GAGCGCAGCC AGAGAAACAG AGAAACAGCT CCCCCCTCTG 
CCAGAGCCCA AACCTGTGTA CGCTCAGCCG GGCCAGCCGG ACGTGGACCT GCCTGTTAGT 
CCGGCAGATG CTCCTGTGCC CAGCGTCACA CATGATGACA GCATCTTACG GCCGAGCATG 
AAGCTGGTGA AGTTCAAGAA GGGCGAGAGC GTGGGCTTGA GGCTCGCTGG GGGGAACGAC 
GTTGGGATAT TTGTGGCGGG AGTTCTGGAG GACAGTCCTG CTGCTAAAGA GGGCCTGGAA 
GAGGGAGACC AGATTCTCAG GGTAAATAAT GTGGATTTTG CAAATATCAT CCGAGAGGAG 
GCAGTGCTGT TCCTGCTGGA TCTGCCCAAA GGAGAGGAGG TCACCATCCT GGCCCAGAAG 
AAGAAAGACG TGTACCGCCG CATTGTAGAG TCTGACGTGG GAGACTCGTT CTACATCAGG 
ACTCATTTTG AATATGAGAA GGAGTCGCCC TATGGCCTGA GCTTCAATAA GGGGGAGGTC 
TTCAGAGTCG TCGACACCCT TTACAATGGC AAACTGGGCT CCTGGCTCGC CATCCGCATC 
GGCAAAAACC ACCAGGAGGT GGAGCGGGGC ATCATCCCCA ACAAGAACAG GGCTGAGCAG 
TTATCAAGTG TCCAGTACAC TCTCCCAAAG ACTCCAGGAG GGGATCGCGC CGACTTCTGG 
AGATTCAGAG GCCTCCGCAG CTCCAAACGA AACCTGAGGA AGAGCAGAGA AGACCTGTCT 
GCTCAGCCGG TCCAAACCAA ATTCCCTGCT TATGAAAGAG TGGTGCTTAG AGAAGCTGGT 
TTCTTGAGGC CAGTGGTCAT TTTCGGACCG ATCGCGGATG TGGCTAGAGA GAAGCTGTCC 
AGAGAGGAGA CTGACCTCTT CGAACTTGCA AAGAGTGAAC CCAGAGATGC GGGCACAGAC 
CAGCGCAGCT CCGGAATCAT CCGTCTCCAT ACCATTAAAC AGATCATTGA CCGAGACAAG 
CACGCGGTGC TGGACATCAC CCCTAATGCG GTGGACCGTC TGAACTACGC TCAGTGGTAC 
CCCATCGTGG TGTTTCTGAA TCCAGACAGC AAACAGGGCG TCAAGAACAT GAGGACCAGG 
CTCTGTCCCG AGTCCAGGAA GAGCGCCCGA AAGCTCTACG AGCGTGCTCT CAAACTGAGG 
AAGAACAACC ATCACCTTTT CACCACCACT ATTAACTTGA ACAACATGAA TGACGGGTGG 
TACGGTGCTT TGAAAGAGAG CATCCAGCAG CAGCAGAACC AGTTGGTCTG GGTCTCAGAG 
GGCAAGGCCG ACGGCGCTCC TGACGATGAC CTTGACATCC ATGACGACCG CCTCTCCTAC 
CTCTCGGCTC CGGGCAGCGA GTACAGCATG TACAGCACCG ACAGCCGCCA CACGTCCGAC 
TATGAGGACA CCGACACGGA GGGTGGTGCC TACACCGACC AGGAGCTGGA CGAGACCATG 
AACGATGAGG TGGGCCTGCC TAGCGAGCCT GCCATCACAC GCTCATCTGA GCCTGTGCGA 
GAGGACCCGC CAGTCATTCA GGACGCAGCT GGATACCCCA GCTACCAGCA TGCGGTACCG 
CAACCTGAAG CGGTGAGCCG CATTGACCCG GCCGGGTTTA AGATGGCAGC GCCGCAGCAG 
CAAGCTGAGG CTGCTCTGGC CTCGCCGCCC CCTCCCTCTG CAGCGGTCCA GCCCACCGCA 
ACACTAGCGG GTACGAACCC CGAGGAGCCG CCTGGCACCC CTCAGGCCGA CTCCCTTAAC 
AGCCCCGTCC CTGTTCCCGA CCCCCAGCCC GAACCTGAGC TCGCTCAGCC CCCAACACAC 
GACCCCCACC AGTCGCTTCC TCCTGGCCCA GATCCAAAGA TGTATAAAAA GGATCTGTAC 
AGCATGGACG AGCCGGTGCG AGTGACCCAC GGCGTGAAGC CTCCCCCTCC CCAGCAGCAG 
CAGCAGCCTT TAGGCCCCCC AACCTCGCTC TCCTACAGCC ACCAGCCTGT CTACCAGGAC 
CAACAGCCAT ACCGCCAGTA CGACCACCCG CCTTATGGCT ATGACGGTGG CGGCTACGCA 
CAACCAAAGC CTCACAACTA CGACCCGCAC CTGCACTACG ACAACCGTGT GCCTCACTAT 
AACGAGCAGT GGCCCACGTA CGACCAGCAG CAGCAGCAGC AGACTTCCCC CCAGCCGCCA 
CCTGCGTCCG GCTTCCCTCA GGGCCACCAG CCTCCGCCTC CACCACCGAT GGGATATGAG 
CCACGCTCCC CATACGAGGA CGGACCTAAC AGGGATTTCA GCCCTCCTCA GTCCCAGTAT 
GACGGTGTGT CCCCAATGGG CTACGATCAC CGGCCGCGGC ACCCCAAACC TGCACCGGTC 
CGATATGAAG AGCCTCCACC TCCACCTCCT CCGGTGTCCT ACGATGCACG GTCACCTTTC 
GAACCCGAGC CGCATGCTTT CCCAGGCAAC ACACATCGCT CGCCAGACCC ACCGAAACAG 
TATTATGGAG ACGCCGCTAT GAGGCCCTTG TACAACCCTG GACCTCCGAA CAGAGTGTAC 
AAACATGAGC CGGTGAACTC TGAGCCTCCT GTACCGCCTC CCAAACCTGA ATCTGTCCTG 
TCTCCAGGAG AACCGCCCCA TTCTGCAGCT TCCAAACCAT TGCCGCCTCC TCCCAGAGAT 
GATGACGATG ATGATGACGA TGATGATGAC CCTGCCATGA AGCCCCAGTC TGTGCTCAAC 
AGGGTCAAAA TGTTCGAAAA CAAACGCTCT GTGTCGGTAG ACCGAGCCAA GGACGCACCT 
GACGTCACTG GAATTAGGCC CACAGATCTC CCCAAACCTG CGAGCACCCC TGGACCTGTG 
CTCAAAGCCA ACTCTCTCAG CAACCTGGAG CAGGACAAAC CCTCCTACAG AGCTCCAGAG 
CCGCAGAAAC CTCAGACGCG TGTTGGGGAC GATGTGGTTC GCTCCAACCA CTACGACCCT 
GATGATGATG AAGAGTACTA CAGAAAACAG CTCTCATTTT TCGACCGCCG CAGTTTCGAC 
AGTAAGGTCA TGAATCCACC CAGCGCTGGA GTAAACCGCT TCCATGAGCC GCCCAAACCA 
CCCCAGACAC AGATCGCTTA TCCTTTCGCT AGGACGGAGT CTGTGGAGAA GGTGAGTCCT 
GTGGACAAGA GATACGAGCC TCTGCCGCCA GTCAACCCTT CACCTGCTCC ATACAGCCAG 
AACACACCTG TCGCTCCGCC CACATCCCTG CCCAAACTCA CCAACATCGA GGTGAACTCT 
CTGCCAGATC CTCACAGCTC TCCCAAAGCC AAACCGGACC TGTCTGCTCT GAGAGCTCCG 
ACCCGGGACG AGCCCATCCA GAGCAGCTAC TTGCCCCCCA AGTCCACCAT CAATGGCATG 
GATGCTCCTC CCAAAACACT CGGCGTGCCG ACCAGCTACA ACCGCTACGT CCCCAAGCCT 
TACACCAGCT CGGCGCGACC CTTCGAGCGC AAGTTCGAGA GTCCGAAATT CAACCACAAC 
CTGCTTCCCA ACGACACGCA GTCCAAACCC AGTGTCAACA ACAACCTGAA GCCTCAGATC 
TCCCCACAAC CCCAGGACAC GGACAGCGGC GTCGACACCT TCACTCGCAC CATGGACAAC 
AGGCCAAAAT ATCAGCACAA CAACATCAAC GCCATTCCCA AAGCCGTCCC AGTCAGCCCC 
AGTGCACTGG ATGATGATGA AGAAGATGAG GGACACACAG TTGTGGCCAC GGCGCGGGGC 
ATCTTTAACT GTAATGGCGG GGTGTTGAGC TCTATAGAGA CGGGCGTCAG CATCATTATC 
CCACAGGGAG CCATTCCTGA TAGTGTGGAG CAGGAGATCT ACTTCAAAGT GTGCCGGGAC 
AACAGCATCC TGCCTCCTCT GGATAAGGAG AAAGGTGAAA CTCTGCTGAG TCCGCTGGTG 
ATGTGTGGTC CTCACGGCCT GAAGTTCCTG AAGCCTGTGG AGCTGCGCTT GCCACACTGT 
GCGTCTATGA CCCCTGATGG TGACCCCAAA TGCTGGCAAA ACAAGTGTCT ACCAGGAGAC 
CCCAATTACC TGGTGGGCGC AAACTGTGTG TCTGTGCTGA TCGACCACTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209660.1
CDS	115..6168
Translation	MLLSVFGGPVEEVLAGLLKLKMFGSGSKKWFIKKNKRQRTKVQRKPASGRRNKRVSRVLKRHKEVIVKQLDVSKVLPTLVYERVFTLGEYKEILGQDSSGKRAELFLDLLSQKEPAAFSAFCGVLEEVCPHLLTCFLLDGTDGSAGENKKQGQTKPAHSQQNALSKGQDTVCFPPMYTDPLFDTRVFPKHDKDKYGPAVYYPKFNAYIPNPDKVSVLDESEGSGHGKPSLRRIKGRIHRSKSLDSIDLLDSNSAAMDETVIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGLLQENDRVVMVNAVSMDNVDHAYAVQQLRKSGKNAKITIRRKRKVQIPSGRPGERETMSEHDEDDDSYEDEIYESRSARSGPSAYSGGGGGGGGGGASGRRSGRGDRLSGRRERDRERSGSRERSLSPRSDRHSVSSNLPPRPAKVTLVKSRKNEAEYGLRLASHIFVKDISPESLAARDGNIQEGDVVLKINGTVTENLSLIDAKKLIERSKGKLKMVVQRDERATLLNIPDLDDSIPSANASDRDDISDIHSVASDHSNRSHDRKRSSRSRSPDRRSEPSDHSRHSPPQISNGRATGPTAQRTRPVHRSRDEDRISKAGPLPLPAKMVEEMPKEQSAARETEKQLPPLPEPKPVYAQPGQPDVDLPVSPADAPVPSVTHDDSILRPSMKLVKFKKGESVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFANIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHQEVERGIIPNKNRAEQLSSVQYTLPKTPGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVVIFGPIADVAREKLSREETDLFELAKSEPRDAGTDQRSSGIIRLHTIKQIIDRDKHAVLDITPNAVDRLNYAQWYPIVVFLNPDSKQGVKNMRTRLCPESRKSARKLYERALKLRKNNHHLFTTTINLNNMNDGWYGALKESIQQQQNQLVWVSEGKADGAPDDDLDIHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETMNDEVGLPSEPAITRSSEPVREDPPVIQDAAGYPSYQHAVPQPEAVSRIDPAGFKMAAPQQQAEAALASPPPPSAAVQPTATLAGTNPEEPPGTPQADSLNSPVPVPDPQPEPELAQPPTHDPHQSLPPGPDPKMYKKDLYSMDEPVRVTHGVKPPPPQQQQQPLGPPTSLSYSHQPVYQDQQPYRQYDHPPYGYDGGGYAQPKPHNYDPHLHYDNRVPHYNEQWPTYDQQQQQQTSPQPPPASGFPQGHQPPPPPPMGYEPRSPYEDGPNRDFSPPQSQYDGVSPMGYDHRPRHPKPAPVRYEEPPPPPPPVSYDARSPFEPEPHAFPGNTHRSPDPPKQYYGDAAMRPLYNPGPPNRVYKHEPVNSEPPVPPPKPESVLSPGEPPHSAASKPLPPPPRDDDDDDDDDDDPAMKPQSVLNRVKMFENKRSVSVDRAKDAPDVTGIRPTDLPKPASTPGPVLKANSLSNLEQDKPSYRAPEPQKPQTRVGDDVVRSNHYDPDDDEEYYRKQLSFFDRRSFDSKVMNPPSAGVNRFHEPPKPPQTQIAYPFARTESVEKVSPVDKRYEPLPPVNPSPAPYSQNTPVAPPTSLPKLTNIEVNSLPDPHSSPKAKPDLSALRAPTRDEPIQSSYLPPKSTINGMDAPPKTLGVPTSYNRYVPKPYTSSARPFERKFESPKFNHNLLPNDTQSKPSVNNNLKPQISPQPQDTDSGVDTFTRTMDNRPKYQHNNINAIPKAVPVSPSALDDDEEDEGHTVVATARGIFNCNGGVLSSIETGVSIIIPQGAIPDSVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGDPKCWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_017354171.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tight junction protein 1b (tjp1b), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017354171.1

ORF Insert Sequence:

ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
TCCCGCAAGA ATGAAGCAGA ATACGGGCTT CGCCTGGCCA GCCACATCTT CGTGAAGGAC 
ATTTCCCCCG AGAGCCTGGC AGCCCGAGAC GGAAACATCC AAGAGGGAGA CGTGGTGCTT 
AAGATTAATG GCACGGTAAC AGAGAATTTG TCCCTGATCG ATGCTAAGAA GCTGATCGAG 
AGGTCAAAGG GAAAGCTGAA GATGGTGGTG CAGAGAGACG AGCGGGCGAC GCTGCTGAAC 
ATCCCCGATC TGGACGACAG CATACCTTCT GCTAATGCCT CGGACAGAGA TGATATTTCA 
GACATCCACT CCGTGGCCTC TGATCATTCC AACCGATCCC ACGACCGGAA GAGGAGCAGC 
CGCTCTCGTT CTCCAGACCG CCGCTCCGAA CCCTCAGACC ACTCCAGACA CTCTCCACCA 
CAGATCAGCA ACGGCAGGGC CACAGGGCCG ACGGCTCAGA GAACGCGGCC AGTTCACAGG 
AGCCGTGATG AAGACAGGAT CTCCAAGGCC GGGCCGCTTC CTCTACCTGC TAAGATGGTG 
GAGGAGATGC CGAAAGAGCA GAGCGCAGCC AGAGAAACAG AGAAACAGCT CCCCCCTCTG 
CCAGAGCCCA AACCTGTGTA CGCTCAGCCG GGCCAGCCGG ACGTGGACCT GCCTGTTAGT 
CCGGCAGATG CTCCTGTGCC CAGCGTCACA CATGATGACA GCATCTTACG GCCGAGCATG 
AAGCTGGTGA AGTTCAAGAA GGGCGAGAGC GTGGGCTTGA GGCTCGCTGG GGGGAACGAC 
GTTGGGATAT TTGTGGCGGG AGTTCTGGAG GACAGTCCTG CTGCTAAAGA GGGCCTGGAA 
GAGGGAGACC AGATTCTCAG GGTAAATAAT GTGGATTTTG CAAATATCAT CCGAGAGGAG 
GCAGTGCTGT TCCTGCTGGA TCTGCCCAAA GGAGAGGAGG TCACCATCCT GGCCCAGAAG 
AAGAAAGACG TGTACCGCCG CATTGTAGAG TCTGACGTGG GAGACTCGTT CTACATCAGG 
ACTCATTTTG AATATGAGAA GGAGTCGCCC TATGGCCTGA GCTTCAATAA GGGGGAGGTC 
TTCAGAGTCG TCGACACCCT TTACAATGGC AAACTGGGCT CCTGGCTCGC CATCCGCATC 
GGCAAAAACC ACCAGGAGGT GGAGCGGGGC ATCATCCCCA ACAAGAACAG GGCTGAGCAG 
TTATCAAGTG TCCAGTACAC TCTCCCAAAG ACTCCAGGAG GGGATCGCGC CGACTTCTGG 
AGATTCAGAG GCCTCCGCAG CTCCAAACGA AACCTGAGGA AGAGCAGAGA AGACCTGTCT 
GCTCAGCCGG TCCAAACCAA ATTCCCTGCT TATGAAAGAG TGGTGCTTAG AGAAGCTGGT 
TTCTTGAGGC CAGTGGTCAT TTTCGGACCG ATCGCGGATG TGGCTAGAGA GAAGCTGTCC 
AGAGAGGAGA CTGACCTCTT CGAACTTGCA AAGAGTGAAC CCAGAGATGC GGGCACAGAC 
CAGCGCAGCT CCGGAATCAT CCGTCTCCAT ACCATTAAAC AGATCATTGA CCGAGACAAG 
CACGCGGTGC TGGACATCAC CCCTAATGCG GTGGACCGTC TGAACTACGC TCAGTGGTAC 
CCCATCGTGG TGTTTCTGAA TCCAGACAGC AAACAGGGCG TCAAGAACAT GAGGACCAGG 
CTCTGTCCCG AGTCCAGGAA GAGCGCCCGA AAGCTCTACG AGCGTGCTCT CAAACTGAGG 
AAGAACAACC ATCACCTTTT CACCACCACT ATTAACTTGA ACAACATGAA TGACGGGTGG 
TACGGTGCTT TGAAAGAGAG CATCCAGCAG CAGCAGAACC AGTTGGTCTG GGTCTCAGAG 
GGCAAGGCCG ACGGCGCTCC TGACGATGAC CTTGACATCC ATGACGACCG CCTCTCCTAC 
CTCTCGGCTC CGGGCAGCGA GTACAGCATG TACAGCACCG ACAGCCGCCA CACGTCCGAC 
TATGAGGACA CCGACACGGA GGGTGGTGCC TACACCGACC AGGAGCTGGA CGAGACCATG 
AACGATGAGG TGGGCCTGCC TAGCGAGCCT GCCATCACAC GCTCATCTGA GCCTGTGCGA 
GAGGACCCGC CAGTCATTCA GGACGCAGCT GGATACCCCA GCTACCAGCA TGCGGTACCG 
CAACCTGAAG CGGTGAGCCG CATTGACCCG GCCGGGTTTA AGATGGCAGC GCCGCAGCAG 
CAAGCTGAGG CTGCTCTGGC CTCGCCGCCC CCTCCCTCTG CAGCGGTCCA GCCCACCGCA 
ACACTAGCGG GTACGAACCC CGAGGAGCCG CCTGGCACCC CTCAGGCCGA CTCCCTTAAC 
AGCCCCGTCC CTGTTCCCGA CCCCCAGCCC GAACCTGAGC TCGCTCAGCC CCCAACACAC 
GACCCCCACC AGTCGCTTCC TCCTGGCCCA GATCCAAAGA TGTATAAAAA GGATCTGTAC 
AGCATGGACG AGCCGGTGCG AGTGACCCAC GGCGTGAAGC CTCCCCCTCC CCAGCAGCAG 
CAGCAGCCTT TAGGCCCCCC AACCTCGCTC TCCTACAGCC ACCAGCCTGT CTACCAGGAC 
CAACAGCCAT ACCGCCAGTA CGACCACCCG CCTTATGGCT ATGACGGTGG CGGCTACGCA 
CAACCAAAGC CTCACAACTA CGACCCGCAC CTGCACTACG ACAACCGTGT GCCTCACTAT 
AACGAGCAGT GGCCCACGTA CGACCAGCAG CAGCAGCAGC AGACTTCCCC CCAGCCGCCA 
CCTGCGTCCG GCTTCCCTCA GGGCCACCAG CCTCCGCCTC CACCACCGAT GGGATATGAG 
CCACGCTCCC CATACGAGGA CGGACCTAAC AGGGATTTCA GCCCTCCTCA GTCCCAGTAT 
GACGGTGTGT CCCCAATGGG CTACGATCAC CGGCCGCGGC ACCCCAAACC TGCACCGGTC 
CGATATGAAG AGCCTCCACC TCCACCTCCT CCGGTGTCCT ACGATGCACG GTCACCTTTC 
GAACCCGAGC CGCATGCTTT CCCAGGCAAC ACACATCGCT CGCCAGACCC ACCGAAACAG 
TATTATGGAG ACGCCGCTAT GAGGCCCTTG TACAACCCTG GACCTCCGAA CAGAGTGTAC 
AAACATGAGC CGGTGAACTC TGAGCCTCCT GTACCGCCTC CCAAACCTGA ATCTGTCCTG 
TCTCCAGGAG AACCGCCCCA TTCTGCAGCT TCCAAACCAT TGCCGCCTCC TCCCAGAGAT 
GATGACGATG ATGATGACGA TGATGATGAC CCTGCCATGA AGCCCCAGTC TGTGCTCAAC 
AGGGTCAAAA TGTTCGAAAA CAAACGCTCT GTGTCGGTAG ACCGAGCCAA GGACGCACCT 
GACGTCACTG GAATTAGGCC CACAGATCTC CCCAAACCTG CGAGCACCCC TGGACCTGTG 
CTCAAAGCCA ACTCTCTCAG CAACCTGGAG CAGGACAAAC CCTCCTACAG AGCTCCAGAG 
CCGCAGAAAC CTCAGACGCG TGTTGGGGAC GATGTGGTTC GCTCCAACCA CTACGACCCT 
GATGATGATG AAGAGTACTA CAGAAAACAG CTCTCATTTT TCGACCGCCG CAGTTTCGAC 
AGTAAGGTCA TGAATCCACC CAGCGCTGGA GTAAACCGCT TCCATGAGCC GCCCAAACCA 
CCCCAGACAC AGATCGCTTA TCCTTTCGCT AGGACGGAGT CTGTGGAGAA GGTGAGTCCT 
GTGGACAAGA GATACGAGCC TCTGCCGCCA GTCAACCCTT CACCTGCTCC ATACAGCCAG 
AACACACCTG TCGCTCCGCC CACATCCCTG CCCAAACTCA CCAACATCGA GGTGAACTCT 
CTGCCAGATC CTCACAGCTC TCCCAAAGCC AAACCGGACC TGTCTGCTCT GAGAGCTCCG 
ACCCGGGACG AGCCCATCCA GAGCAGCTAC TTGCCCCCCA AGTCCACCAT CAATGGCATG 
GATGCTCCTC CCAAAACACT CGGCGTGCCG ACCAGCTACA ACCGCTACGT CCCCAAGCCT 
TACACCAGCT CGGCGCGACC CTTCGAGCGC AAGTTCGAGA GTCCGAAATT CAACCACAAC 
CTGCTTCCCA ACGACACGCA GTCCAAACCC AGTGTCAACA ACAACCTGAA GCCTCAGATC 
TCCCCACAAC CCCAGGACAC GGACAGCGGC GTCGACACCT TCACTCGCAC CATGGACAAC 
AGGCCAAAAT ATCAGCACAA CAACATCAAC GCCATTCCCA AAGCCGTCCC AGTCAGCCCC 
AGTGCACTGG ATGATGATGA AGAAGATGAG GGACACACAG TTGTGGCCAC GGCGCGGGGC 
ATCTTTAACT GTAATGGCGG GGTGTTGAGC TCTATAGAGA CGGGCGTCAG CATCATTATC 
CCACAGGGAG CCATTCCTGA TAGTGTGGAG CAGGAGATCT ACTTCAAAGT GTGCCGGGAC 
AACAGCATCC TGCCTCCTCT GGATAAGGAG AAAGGTGAAA CTCTGCTGAG TCCGCTGGTG 
ATGTGTGGTC CTCACGGCCT GAAGTTCCTG AAGCCTGTGG AGCTGCGCTT GCCACACTGT 
GCGTCTATGA CCCCTGATGG TGACCCCAAA TGCTGGCAAA ACAAGTGTCT ACCAGGAGAC 
CCCAATTACC TGGTGGGCGC AAACTGTGTG TCTGTGCTGA TCGACCACTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp1b ( XM_017354172.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017209661.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X8, mRNA.

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CloneID	ODa57883
Clone ID Related Accession (Same CDS sequence)	XM_017354172.1 , XM_017354172.2
Accession Version	XM_017354172.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6048bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tight junction protein ZO-1 isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
TCCCGCAAGA ATGAAGCAGA ATACGGGCTT CGCCTGGCCA GCCACATCTT CGTGAAGGAC 
ATTTCCCCCG AGAGCCTGGC AGCCCGAGAC GGAAACATCC AAGAGGGAGA CGTGGTGCTT 
AAGAAGCTGA TCGAGAGGTC AAAGGGAAAG CTGAAGATGG TGGTGCAGAG AGACGAGCGG 
GCGACGCTGC TGAACATCCC CGATCTGGAC GACAGCATAC CTTCTGCTAA TGCCTCGGAC 
AGAGATGATA TTTCAGACAT CCACTCCGTG GCCTCTGATC ATTCCAACCG ATCCCACGAC 
CGGAAGAGGA GCAGCCGCTC TCGTTCTCCA GACCGCCGCT CCGAACCCTC AGACCACTCC 
AGACACTCTC CACCACAGAT CAGCAACGGC AGGGCCACAG GGCCGACGGC TCAGAGAACG 
CGGCCAGTTC ACAGGAGCCG TGATGAAGAC AGGATCTCCA AGGCCGGGCC GCTTCCTCTA 
CCTGCTAAGA TGGTGGAGGA GATGCCGAAA GAGCAGAGCG CAGCCAGAGA AACAGAGAAA 
CAGCTCCCCC CTCTGCCAGA GCCCAAACCT GTGTACGCTC AGCCGGGCCA GCCGGACGTG 
GACCTGCCTG TTAGTCCGGC AGATGCTCCT GTGCCCAGCG TCACACATGA TGACAGCATC 
TTACGGCCGA GCATGAAGCT GGTGAAGTTC AAGAAGGGCG AGAGCGTGGG CTTGAGGCTC 
GCTGGGGGGA ACGACGTTGG GATATTTGTG GCGGGAGTTC TGGAGGACAG TCCTGCTGCT 
AAAGAGGGCC TGGAAGAGGG AGACCAGATT CTCAGGGTAA ATAATGTGGA TTTTGCAAAT 
ATCATCCGAG AGGAGGCAGT GCTGTTCCTG CTGGATCTGC CCAAAGGAGA GGAGGTCACC 
ATCCTGGCCC AGAAGAAGAA AGACGTGTAC CGCCGCATTG TAGAGTCTGA CGTGGGAGAC 
TCGTTCTACA TCAGGACTCA TTTTGAATAT GAGAAGGAGT CGCCCTATGG CCTGAGCTTC 
AATAAGGGGG AGGTCTTCAG AGTCGTCGAC ACCCTTTACA ATGGCAAACT GGGCTCCTGG 
CTCGCCATCC GCATCGGCAA AAACCACCAG GAGGTGGAGC GGGGCATCAT CCCCAACAAG 
AACAGGGCTG AGCAGTTATC AAGTGTCCAG TACACTCTCC CAAAGACTCC AGGAGGGGAT 
CGCGCCGACT TCTGGAGATT CAGAGGCCTC CGCAGCTCCA AACGAAACCT GAGGAAGAGC 
AGAGAAGACC TGTCTGCTCA GCCGGTCCAA ACCAAATTCC CTGCTTATGA AAGAGTGGTG 
CTTAGAGAAG CTGGTTTCTT GAGGCCAGTG GTCATTTTCG GACCGATCGC GGATGTGGCT 
AGAGAGAAGC TGTCCAGAGA GGAGACTGAC CTCTTCGAAC TTGCAAAGAG TGAACCCAGA 
GATGCGGGCA CAGACCAGCG CAGCTCCGGA ATCATCCGTC TCCATACCAT TAAACAGATC 
ATTGACCGAG ACAAGCACGC GGTGCTGGAC ATCACCCCTA ATGCGGTGGA CCGTCTGAAC 
TACGCTCAGT GGTACCCCAT CGTGGTGTTT CTGAATCCAG ACAGCAAACA GGGCGTCAAG 
AACATGAGGA CCAGGCTCTG TCCCGAGTCC AGGAAGAGCG CCCGAAAGCT CTACGAGCGT 
GCTCTCAAAC TGAGGAAGAA CAACCATCAC CTTTTCACCA CCACTATTAA CTTGAACAAC 
ATGAATGACG GGTGGTACGG TGCTTTGAAA GAGAGCATCC AGCAGCAGCA GAACCAGTTG 
GTCTGGGTCT CAGAGGGCAA GGCCGACGGC GCTCCTGACG ATGACCTTGA CATCCATGAC 
GACCGCCTCT CCTACCTCTC GGCTCCGGGC AGCGAGTACA GCATGTACAG CACCGACAGC 
CGCCACACGT CCGACTATGA GGACACCGAC ACGGAGGGTG GTGCCTACAC CGACCAGGAG 
CTGGACGAGA CCATGAACGA TGAGGTGGGC CTGCCTAGCG AGCCTGCCAT CACACGCTCA 
TCTGAGCCTG TGCGAGAGGA CCCGCCAGTC ATTCAGGACG CAGCTGGATA CCCCAGCTAC 
CAGCATGCGG TACCGCAACC TGAAGCGGTG AGCCGCATTG ACCCGGCCGG GTTTAAGATG 
GCAGCGCCGC AGCAGCAAGC TGAGGCTGCT CTGGCCTCGC CGCCCCCTCC CTCTGCAGCG 
GTCCAGCCCA CCGCAACACT AGCGGGTACG AACCCCGAGG AGCCGCCTGG CACCCCTCAG 
GCCGACTCCC TTAACAGCCC CGTCCCTGTT CCCGACCCCC AGCCCGAACC TGAGCTCGCT 
CAGCCCCCAA CACACGACCC CCACCAGTCG CTTCCTCCTG GCCCAGATCC AAAGATGTAT 
AAAAAGGATC TGTACAGCAT GGACGAGCCG GTGCGAGTGA CCCACGGCGT GAAGCCTCCC 
CCTCCCCAGC AGCAGCAGCA GCCTTTAGGC CCCCCAACCT CGCTCTCCTA CAGCCACCAG 
CCTGTCTACC AGGACCAACA GCCATACCGC CAGTACGACC ACCCGCCTTA TGGCTATGAC 
GGTGGCGGCT ACGCACAACC AAAGCCTCAC AACTACGACC CGCACCTGCA CTACGACAAC 
CGTGTGCCTC ACTATAACGA GCAGTGGCCC ACGTACGACC AGCAGCAGCA GCAGCAGACT 
TCCCCCCAGC CGCCACCTGC GTCCGGCTTC CCTCAGGGCC ACCAGCCTCC GCCTCCACCA 
CCGATGGGAT ATGAGCCACG CTCCCCATAC GAGGACGGAC CTAACAGGGA TTTCAGCCCT 
CCTCAGTCCC AGTATGACGG TGTGTCCCCA ATGGGCTACG ATCACCGGCC GCGGCACCCC 
AAACCTGCAC CGGTCCGATA TGAAGAGCCT CCACCTCCAC CTCCTCCGGT GTCCTACGAT 
GCACGGTCAC CTTTCGAACC CGAGCCGCAT GCTTTCCCAG GCAACACACA TCGCTCGCCA 
GACCCACCGA AACAGTATTA TGGAGACGCC GCTATGAGGC CCTTGTACAA CCCTGGACCT 
CCGAACAGAG TGTACAAACA TGAGCCGGTG AACTCTGAGC CTCCTGTACC GCCTCCCAAA 
CCTGAATCTG TCCTGTCTCC AGGAGAACCG CCCCATTCTG CAGCTTCCAA ACCATTGCCG 
CCTCCTCCCA GAGATGATGA CGATGATGAT GACGATGATG ATGACCCTGC CATGAAGCCC 
CAGTCTGTGC TCAACAGGGT CAAAATGTTC GAAAACAAAC GCTCTGTGTC GGTAGACCGA 
GCCAAGGACG CACCTGACGT CACTGGAATT AGGCCCACAG ATCTCCCCAA ACCTGCGAGC 
ACCCCTGGAC CTGTGCTCAA AGCCAACTCT CTCAGCAACC TGGAGCAGGA CAAACCCTCC 
TACAGAGCTC CAGAGCCGCA GAAACCTCAG ACGCGTGTTG GGGACGATGT GGTTCGCTCC 
AACCACTACG ACCCTGATGA TGATGAAGAG TACTACAGAA AACAGCTCTC ATTTTTCGAC 
CGCCGCAGTT TCGACAGTAA GGTCATGAAT CCACCCAGCG CTGGAGTAAA CCGCTTCCAT 
GAGCCGCCCA AACCACCCCA GACACAGATC GCTTATCCTT TCGCTAGGAC GGAGTCTGTG 
GAGAAGGTGA GTCCTGTGGA CAAGAGATAC GAGCCTCTGC CGCCAGTCAA CCCTTCACCT 
GCTCCATACA GCCAGAACAC ACCTGTCGCT CCGCCCACAT CCCTGCCCAA ACTCACCAAC 
ATCGAGGTGA ACTCTCTGCC AGATCCTCAC AGCTCTCCCA AAGCCAAACC GGACCTGTCT 
GCTCTGAGAG CTCCGACCCG GGACGAGCCC ATCCAGAGCA GCTACTTGCC CCCCAAGTCC 
ACCATCAATG GCATGGATGC TCCTCCCAAA ACACTCGGCG TGCCGACCAG CTACAACCGC 
TACGTCCCCA AGCCTTACAC CAGCTCGGCG CGACCCTTCG AGCGCAAGTT CGAGAGTCCG 
AAATTCAACC ACAACCTGCT TCCCAACGAC ACGCAGTCCA AACCCAGTGT CAACAACAAC 
CTGAAGCCTC AGATCTCCCC ACAACCCCAG GACACGGACA GCGGCGTCGA CACCTTCACT 
CGCACCATGG ACAACAGGCC AAAATATCAG CACAACAACA TCAACGCCAT TCCCAAAGCC 
GTCCCAGTCA GCCCCAGTGC ACTGGATGAT GATGAAGAAG ATGAGGGACA CACAGTTGTG 
GCCACGGCGC GGGGCATCTT TAACTGTAAT GGCGGGGTGT TGAGCTCTAT AGAGACGGGC 
GTCAGCATCA TTATCCCACA GGGAGCCATT CCTGATAGTG TGGAGCAGGA GATCTACTTC 
AAAGTGTGCC GGGACAACAG CATCCTGCCT CCTCTGGATA AGGAGAAAGG TGAAACTCTG 
CTGAGTCCGC TGGTGATGTG TGGTCCTCAC GGCCTGAAGT TCCTGAAGCC TGTGGAGCTG 
CGCTTGCCAC ACTGTGCGTC TATGACCCCT GATGGTTGGT CTTTTGCTCT AAAATCCTCC 
GACTCCTCGT CGGGTGACCC CAAATGCTGG CAAAACAAGT GTCTACCAGG AGACCCCAAT 
TACCTGGTGG GCGCAAACTG TGTGTCTGTG CTGATCGACC ACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209661.1
CDS	115..6162
Translation	MLLSVFGGPVEEVLAGLLKLKMFGSGSKKWFIKKNKRQRTKVQRKPASGRRNKRVSRVLKRHKEVIVKQLDVSKVLPTLVYERVFTLGEYKEILGQDSSGKRAELFLDLLSQKEPAAFSAFCGVLEEVCPHLLTCFLLDGTDGSAGENKKQGQTKPAHSQQNALSKGQDTVCFPPMYTDPLFDTRVFPKHDKDKYGPAVYYPKFNAYIPNPDKVSVLDESEGSGHGKPSLRRIKGRIHRSKSLDSIDLLDSNSAAMDETVIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGLLQENDRVVMVNAVSMDNVDHAYAVQQLRKSGKNAKITIRRKRKVQIPSGRPGERETMSEHDEDDDSYEDEIYESRSARSGPSAYSGGGGGGGGGGASGRRSGRGDRLSGRRERDRERSGSRERSLSPRSDRHSVSSNLPPRPAKVTLVKSRKNEAEYGLRLASHIFVKDISPESLAARDGNIQEGDVVLKKLIERSKGKLKMVVQRDERATLLNIPDLDDSIPSANASDRDDISDIHSVASDHSNRSHDRKRSSRSRSPDRRSEPSDHSRHSPPQISNGRATGPTAQRTRPVHRSRDEDRISKAGPLPLPAKMVEEMPKEQSAARETEKQLPPLPEPKPVYAQPGQPDVDLPVSPADAPVPSVTHDDSILRPSMKLVKFKKGESVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFANIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHQEVERGIIPNKNRAEQLSSVQYTLPKTPGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVVIFGPIADVAREKLSREETDLFELAKSEPRDAGTDQRSSGIIRLHTIKQIIDRDKHAVLDITPNAVDRLNYAQWYPIVVFLNPDSKQGVKNMRTRLCPESRKSARKLYERALKLRKNNHHLFTTTINLNNMNDGWYGALKESIQQQQNQLVWVSEGKADGAPDDDLDIHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETMNDEVGLPSEPAITRSSEPVREDPPVIQDAAGYPSYQHAVPQPEAVSRIDPAGFKMAAPQQQAEAALASPPPPSAAVQPTATLAGTNPEEPPGTPQADSLNSPVPVPDPQPEPELAQPPTHDPHQSLPPGPDPKMYKKDLYSMDEPVRVTHGVKPPPPQQQQQPLGPPTSLSYSHQPVYQDQQPYRQYDHPPYGYDGGGYAQPKPHNYDPHLHYDNRVPHYNEQWPTYDQQQQQQTSPQPPPASGFPQGHQPPPPPPMGYEPRSPYEDGPNRDFSPPQSQYDGVSPMGYDHRPRHPKPAPVRYEEPPPPPPPVSYDARSPFEPEPHAFPGNTHRSPDPPKQYYGDAAMRPLYNPGPPNRVYKHEPVNSEPPVPPPKPESVLSPGEPPHSAASKPLPPPPRDDDDDDDDDDDPAMKPQSVLNRVKMFENKRSVSVDRAKDAPDVTGIRPTDLPKPASTPGPVLKANSLSNLEQDKPSYRAPEPQKPQTRVGDDVVRSNHYDPDDDEEYYRKQLSFFDRRSFDSKVMNPPSAGVNRFHEPPKPPQTQIAYPFARTESVEKVSPVDKRYEPLPPVNPSPAPYSQNTPVAPPTSLPKLTNIEVNSLPDPHSSPKAKPDLSALRAPTRDEPIQSSYLPPKSTINGMDAPPKTLGVPTSYNRYVPKPYTSSARPFERKFESPKFNHNLLPNDTQSKPSVNNNLKPQISPQPQDTDSGVDTFTRTMDNRPKYQHNNINAIPKAVPVSPSALDDDEEDEGHTVVATARGIFNCNGGVLSSIETGVSIIIPQGAIPDSVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKCWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_017354172.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tight junction protein 1b (tjp1b), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017354172.1

ORF Insert Sequence:

ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
TCCCGCAAGA ATGAAGCAGA ATACGGGCTT CGCCTGGCCA GCCACATCTT CGTGAAGGAC 
ATTTCCCCCG AGAGCCTGGC AGCCCGAGAC GGAAACATCC AAGAGGGAGA CGTGGTGCTT 
AAGAAGCTGA TCGAGAGGTC AAAGGGAAAG CTGAAGATGG TGGTGCAGAG AGACGAGCGG 
GCGACGCTGC TGAACATCCC CGATCTGGAC GACAGCATAC CTTCTGCTAA TGCCTCGGAC 
AGAGATGATA TTTCAGACAT CCACTCCGTG GCCTCTGATC ATTCCAACCG ATCCCACGAC 
CGGAAGAGGA GCAGCCGCTC TCGTTCTCCA GACCGCCGCT CCGAACCCTC AGACCACTCC 
AGACACTCTC CACCACAGAT CAGCAACGGC AGGGCCACAG GGCCGACGGC TCAGAGAACG 
CGGCCAGTTC ACAGGAGCCG TGATGAAGAC AGGATCTCCA AGGCCGGGCC GCTTCCTCTA 
CCTGCTAAGA TGGTGGAGGA GATGCCGAAA GAGCAGAGCG CAGCCAGAGA AACAGAGAAA 
CAGCTCCCCC CTCTGCCAGA GCCCAAACCT GTGTACGCTC AGCCGGGCCA GCCGGACGTG 
GACCTGCCTG TTAGTCCGGC AGATGCTCCT GTGCCCAGCG TCACACATGA TGACAGCATC 
TTACGGCCGA GCATGAAGCT GGTGAAGTTC AAGAAGGGCG AGAGCGTGGG CTTGAGGCTC 
GCTGGGGGGA ACGACGTTGG GATATTTGTG GCGGGAGTTC TGGAGGACAG TCCTGCTGCT 
AAAGAGGGCC TGGAAGAGGG AGACCAGATT CTCAGGGTAA ATAATGTGGA TTTTGCAAAT 
ATCATCCGAG AGGAGGCAGT GCTGTTCCTG CTGGATCTGC CCAAAGGAGA GGAGGTCACC 
ATCCTGGCCC AGAAGAAGAA AGACGTGTAC CGCCGCATTG TAGAGTCTGA CGTGGGAGAC 
TCGTTCTACA TCAGGACTCA TTTTGAATAT GAGAAGGAGT CGCCCTATGG CCTGAGCTTC 
AATAAGGGGG AGGTCTTCAG AGTCGTCGAC ACCCTTTACA ATGGCAAACT GGGCTCCTGG 
CTCGCCATCC GCATCGGCAA AAACCACCAG GAGGTGGAGC GGGGCATCAT CCCCAACAAG 
AACAGGGCTG AGCAGTTATC AAGTGTCCAG TACACTCTCC CAAAGACTCC AGGAGGGGAT 
CGCGCCGACT TCTGGAGATT CAGAGGCCTC CGCAGCTCCA AACGAAACCT GAGGAAGAGC 
AGAGAAGACC TGTCTGCTCA GCCGGTCCAA ACCAAATTCC CTGCTTATGA AAGAGTGGTG 
CTTAGAGAAG CTGGTTTCTT GAGGCCAGTG GTCATTTTCG GACCGATCGC GGATGTGGCT 
AGAGAGAAGC TGTCCAGAGA GGAGACTGAC CTCTTCGAAC TTGCAAAGAG TGAACCCAGA 
GATGCGGGCA CAGACCAGCG CAGCTCCGGA ATCATCCGTC TCCATACCAT TAAACAGATC 
ATTGACCGAG ACAAGCACGC GGTGCTGGAC ATCACCCCTA ATGCGGTGGA CCGTCTGAAC 
TACGCTCAGT GGTACCCCAT CGTGGTGTTT CTGAATCCAG ACAGCAAACA GGGCGTCAAG 
AACATGAGGA CCAGGCTCTG TCCCGAGTCC AGGAAGAGCG CCCGAAAGCT CTACGAGCGT 
GCTCTCAAAC TGAGGAAGAA CAACCATCAC CTTTTCACCA CCACTATTAA CTTGAACAAC 
ATGAATGACG GGTGGTACGG TGCTTTGAAA GAGAGCATCC AGCAGCAGCA GAACCAGTTG 
GTCTGGGTCT CAGAGGGCAA GGCCGACGGC GCTCCTGACG ATGACCTTGA CATCCATGAC 
GACCGCCTCT CCTACCTCTC GGCTCCGGGC AGCGAGTACA GCATGTACAG CACCGACAGC 
CGCCACACGT CCGACTATGA GGACACCGAC ACGGAGGGTG GTGCCTACAC CGACCAGGAG 
CTGGACGAGA CCATGAACGA TGAGGTGGGC CTGCCTAGCG AGCCTGCCAT CACACGCTCA 
TCTGAGCCTG TGCGAGAGGA CCCGCCAGTC ATTCAGGACG CAGCTGGATA CCCCAGCTAC 
CAGCATGCGG TACCGCAACC TGAAGCGGTG AGCCGCATTG ACCCGGCCGG GTTTAAGATG 
GCAGCGCCGC AGCAGCAAGC TGAGGCTGCT CTGGCCTCGC CGCCCCCTCC CTCTGCAGCG 
GTCCAGCCCA CCGCAACACT AGCGGGTACG AACCCCGAGG AGCCGCCTGG CACCCCTCAG 
GCCGACTCCC TTAACAGCCC CGTCCCTGTT CCCGACCCCC AGCCCGAACC TGAGCTCGCT 
CAGCCCCCAA CACACGACCC CCACCAGTCG CTTCCTCCTG GCCCAGATCC AAAGATGTAT 
AAAAAGGATC TGTACAGCAT GGACGAGCCG GTGCGAGTGA CCCACGGCGT GAAGCCTCCC 
CCTCCCCAGC AGCAGCAGCA GCCTTTAGGC CCCCCAACCT CGCTCTCCTA CAGCCACCAG 
CCTGTCTACC AGGACCAACA GCCATACCGC CAGTACGACC ACCCGCCTTA TGGCTATGAC 
GGTGGCGGCT ACGCACAACC AAAGCCTCAC AACTACGACC CGCACCTGCA CTACGACAAC 
CGTGTGCCTC ACTATAACGA GCAGTGGCCC ACGTACGACC AGCAGCAGCA GCAGCAGACT 
TCCCCCCAGC CGCCACCTGC GTCCGGCTTC CCTCAGGGCC ACCAGCCTCC GCCTCCACCA 
CCGATGGGAT ATGAGCCACG CTCCCCATAC GAGGACGGAC CTAACAGGGA TTTCAGCCCT 
CCTCAGTCCC AGTATGACGG TGTGTCCCCA ATGGGCTACG ATCACCGGCC GCGGCACCCC 
AAACCTGCAC CGGTCCGATA TGAAGAGCCT CCACCTCCAC CTCCTCCGGT GTCCTACGAT 
GCACGGTCAC CTTTCGAACC CGAGCCGCAT GCTTTCCCAG GCAACACACA TCGCTCGCCA 
GACCCACCGA AACAGTATTA TGGAGACGCC GCTATGAGGC CCTTGTACAA CCCTGGACCT 
CCGAACAGAG TGTACAAACA TGAGCCGGTG AACTCTGAGC CTCCTGTACC GCCTCCCAAA 
CCTGAATCTG TCCTGTCTCC AGGAGAACCG CCCCATTCTG CAGCTTCCAA ACCATTGCCG 
CCTCCTCCCA GAGATGATGA CGATGATGAT GACGATGATG ATGACCCTGC CATGAAGCCC 
CAGTCTGTGC TCAACAGGGT CAAAATGTTC GAAAACAAAC GCTCTGTGTC GGTAGACCGA 
GCCAAGGACG CACCTGACGT CACTGGAATT AGGCCCACAG ATCTCCCCAA ACCTGCGAGC 
ACCCCTGGAC CTGTGCTCAA AGCCAACTCT CTCAGCAACC TGGAGCAGGA CAAACCCTCC 
TACAGAGCTC CAGAGCCGCA GAAACCTCAG ACGCGTGTTG GGGACGATGT GGTTCGCTCC 
AACCACTACG ACCCTGATGA TGATGAAGAG TACTACAGAA AACAGCTCTC ATTTTTCGAC 
CGCCGCAGTT TCGACAGTAA GGTCATGAAT CCACCCAGCG CTGGAGTAAA CCGCTTCCAT 
GAGCCGCCCA AACCACCCCA GACACAGATC GCTTATCCTT TCGCTAGGAC GGAGTCTGTG 
GAGAAGGTGA GTCCTGTGGA CAAGAGATAC GAGCCTCTGC CGCCAGTCAA CCCTTCACCT 
GCTCCATACA GCCAGAACAC ACCTGTCGCT CCGCCCACAT CCCTGCCCAA ACTCACCAAC 
ATCGAGGTGA ACTCTCTGCC AGATCCTCAC AGCTCTCCCA AAGCCAAACC GGACCTGTCT 
GCTCTGAGAG CTCCGACCCG GGACGAGCCC ATCCAGAGCA GCTACTTGCC CCCCAAGTCC 
ACCATCAATG GCATGGATGC TCCTCCCAAA ACACTCGGCG TGCCGACCAG CTACAACCGC 
TACGTCCCCA AGCCTTACAC CAGCTCGGCG CGACCCTTCG AGCGCAAGTT CGAGAGTCCG 
AAATTCAACC ACAACCTGCT TCCCAACGAC ACGCAGTCCA AACCCAGTGT CAACAACAAC 
CTGAAGCCTC AGATCTCCCC ACAACCCCAG GACACGGACA GCGGCGTCGA CACCTTCACT 
CGCACCATGG ACAACAGGCC AAAATATCAG CACAACAACA TCAACGCCAT TCCCAAAGCC 
GTCCCAGTCA GCCCCAGTGC ACTGGATGAT GATGAAGAAG ATGAGGGACA CACAGTTGTG 
GCCACGGCGC GGGGCATCTT TAACTGTAAT GGCGGGGTGT TGAGCTCTAT AGAGACGGGC 
GTCAGCATCA TTATCCCACA GGGAGCCATT CCTGATAGTG TGGAGCAGGA GATCTACTTC 
AAAGTGTGCC GGGACAACAG CATCCTGCCT CCTCTGGATA AGGAGAAAGG TGAAACTCTG 
CTGAGTCCGC TGGTGATGTG TGGTCCTCAC GGCCTGAAGT TCCTGAAGCC TGTGGAGCTG 
CGCTTGCCAC ACTGTGCGTC TATGACCCCT GATGGTTGGT CTTTTGCTCT AAAATCCTCC 
GACTCCTCGT CGGGTGACCC CAAATGCTGG CAAAACAAGT GTCTACCAGG AGACCCCAAT 
TACCTGGTGG GCGCAAACTG TGTGTCTGTG CTGATCGACC ACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp1b ( XM_017354172.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017209661.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X14, mRNA.

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CloneID	ODa57883
Clone ID Related Accession (Same CDS sequence)	XM_017354172.1 , XM_017354172.2
Accession Version	XM_017354172.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6048bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tight junction protein ZO-1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017354172.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
TCCCGCAAGA ATGAAGCAGA ATACGGGCTT CGCCTGGCCA GCCACATCTT CGTGAAGGAC 
ATTTCCCCCG AGAGCCTGGC AGCCCGAGAC GGAAACATCC AAGAGGGAGA CGTGGTGCTT 
AAGAAGCTGA TCGAGAGGTC AAAGGGAAAG CTGAAGATGG TGGTGCAGAG AGACGAGCGG 
GCGACGCTGC TGAACATCCC CGATCTGGAC GACAGCATAC CTTCTGCTAA TGCCTCGGAC 
AGAGATGATA TTTCAGACAT CCACTCCGTG GCCTCTGATC ATTCCAACCG ATCCCACGAC 
CGGAAGAGGA GCAGCCGCTC TCGTTCTCCA GACCGCCGCT CCGAACCCTC AGACCACTCC 
AGACACTCTC CACCACAGAT CAGCAACGGC AGGGCCACAG GGCCGACGGC TCAGAGAACG 
CGGCCAGTTC ACAGGAGCCG TGATGAAGAC AGGATCTCCA AGGCCGGGCC GCTTCCTCTA 
CCTGCTAAGA TGGTGGAGGA GATGCCGAAA GAGCAGAGCG CAGCCAGAGA AACAGAGAAA 
CAGCTCCCCC CTCTGCCAGA GCCCAAACCT GTGTACGCTC AGCCGGGCCA GCCGGACGTG 
GACCTGCCTG TTAGTCCGGC AGATGCTCCT GTGCCCAGCG TCACACATGA TGACAGCATC 
TTACGGCCGA GCATGAAGCT GGTGAAGTTC AAGAAGGGCG AGAGCGTGGG CTTGAGGCTC 
GCTGGGGGGA ACGACGTTGG GATATTTGTG GCGGGAGTTC TGGAGGACAG TCCTGCTGCT 
AAAGAGGGCC TGGAAGAGGG AGACCAGATT CTCAGGGTAA ATAATGTGGA TTTTGCAAAT 
ATCATCCGAG AGGAGGCAGT GCTGTTCCTG CTGGATCTGC CCAAAGGAGA GGAGGTCACC 
ATCCTGGCCC AGAAGAAGAA AGACGTGTAC CGCCGCATTG TAGAGTCTGA CGTGGGAGAC 
TCGTTCTACA TCAGGACTCA TTTTGAATAT GAGAAGGAGT CGCCCTATGG CCTGAGCTTC 
AATAAGGGGG AGGTCTTCAG AGTCGTCGAC ACCCTTTACA ATGGCAAACT GGGCTCCTGG 
CTCGCCATCC GCATCGGCAA AAACCACCAG GAGGTGGAGC GGGGCATCAT CCCCAACAAG 
AACAGGGCTG AGCAGTTATC AAGTGTCCAG TACACTCTCC CAAAGACTCC AGGAGGGGAT 
CGCGCCGACT TCTGGAGATT CAGAGGCCTC CGCAGCTCCA AACGAAACCT GAGGAAGAGC 
AGAGAAGACC TGTCTGCTCA GCCGGTCCAA ACCAAATTCC CTGCTTATGA AAGAGTGGTG 
CTTAGAGAAG CTGGTTTCTT GAGGCCAGTG GTCATTTTCG GACCGATCGC GGATGTGGCT 
AGAGAGAAGC TGTCCAGAGA GGAGACTGAC CTCTTCGAAC TTGCAAAGAG TGAACCCAGA 
GATGCGGGCA CAGACCAGCG CAGCTCCGGA ATCATCCGTC TCCATACCAT TAAACAGATC 
ATTGACCGAG ACAAGCACGC GGTGCTGGAC ATCACCCCTA ATGCGGTGGA CCGTCTGAAC 
TACGCTCAGT GGTACCCCAT CGTGGTGTTT CTGAATCCAG ACAGCAAACA GGGCGTCAAG 
AACATGAGGA CCAGGCTCTG TCCCGAGTCC AGGAAGAGCG CCCGAAAGCT CTACGAGCGT 
GCTCTCAAAC TGAGGAAGAA CAACCATCAC CTTTTCACCA CCACTATTAA CTTGAACAAC 
ATGAATGACG GGTGGTACGG TGCTTTGAAA GAGAGCATCC AGCAGCAGCA GAACCAGTTG 
GTCTGGGTCT CAGAGGGCAA GGCCGACGGC GCTCCTGACG ATGACCTTGA CATCCATGAC 
GACCGCCTCT CCTACCTCTC GGCTCCGGGC AGCGAGTACA GCATGTACAG CACCGACAGC 
CGCCACACGT CCGACTATGA GGACACCGAC ACGGAGGGTG GTGCCTACAC CGACCAGGAG 
CTGGACGAGA CCATGAACGA TGAGGTGGGC CTGCCTAGCG AGCCTGCCAT CACACGCTCA 
TCTGAGCCTG TGCGAGAGGA CCCGCCAGTC ATTCAGGACG CAGCTGGATA CCCCAGCTAC 
CAGCATGCGG TACCGCAACC TGAAGCGGTG AGCCGCATTG ACCCGGCCGG GTTTAAGATG 
GCAGCGCCGC AGCAGCAAGC TGAGGCTGCT CTGGCCTCGC CGCCCCCTCC CTCTGCAGCG 
GTCCAGCCCA CCGCAACACT AGCGGGTACG AACCCCGAGG AGCCGCCTGG CACCCCTCAG 
GCCGACTCCC TTAACAGCCC CGTCCCTGTT CCCGACCCCC AGCCCGAACC TGAGCTCGCT 
CAGCCCCCAA CACACGACCC CCACCAGTCG CTTCCTCCTG GCCCAGATCC AAAGATGTAT 
AAAAAGGATC TGTACAGCAT GGACGAGCCG GTGCGAGTGA CCCACGGCGT GAAGCCTCCC 
CCTCCCCAGC AGCAGCAGCA GCCTTTAGGC CCCCCAACCT CGCTCTCCTA CAGCCACCAG 
CCTGTCTACC AGGACCAACA GCCATACCGC CAGTACGACC ACCCGCCTTA TGGCTATGAC 
GGTGGCGGCT ACGCACAACC AAAGCCTCAC AACTACGACC CGCACCTGCA CTACGACAAC 
CGTGTGCCTC ACTATAACGA GCAGTGGCCC ACGTACGACC AGCAGCAGCA GCAGCAGACT 
TCCCCCCAGC CGCCACCTGC GTCCGGCTTC CCTCAGGGCC ACCAGCCTCC GCCTCCACCA 
CCGATGGGAT ATGAGCCACG CTCCCCATAC GAGGACGGAC CTAACAGGGA TTTCAGCCCT 
CCTCAGTCCC AGTATGACGG TGTGTCCCCA ATGGGCTACG ATCACCGGCC GCGGCACCCC 
AAACCTGCAC CGGTCCGATA TGAAGAGCCT CCACCTCCAC CTCCTCCGGT GTCCTACGAT 
GCACGGTCAC CTTTCGAACC CGAGCCGCAT GCTTTCCCAG GCAACACACA TCGCTCGCCA 
GACCCACCGA AACAGTATTA TGGAGACGCC GCTATGAGGC CCTTGTACAA CCCTGGACCT 
CCGAACAGAG TGTACAAACA TGAGCCGGTG AACTCTGAGC CTCCTGTACC GCCTCCCAAA 
CCTGAATCTG TCCTGTCTCC AGGAGAACCG CCCCATTCTG CAGCTTCCAA ACCATTGCCG 
CCTCCTCCCA GAGATGATGA CGATGATGAT GACGATGATG ATGACCCTGC CATGAAGCCC 
CAGTCTGTGC TCAACAGGGT CAAAATGTTC GAAAACAAAC GCTCTGTGTC GGTAGACCGA 
GCCAAGGACG CACCTGACGT CACTGGAATT AGGCCCACAG ATCTCCCCAA ACCTGCGAGC 
ACCCCTGGAC CTGTGCTCAA AGCCAACTCT CTCAGCAACC TGGAGCAGGA CAAACCCTCC 
TACAGAGCTC CAGAGCCGCA GAAACCTCAG ACGCGTGTTG GGGACGATGT GGTTCGCTCC 
AACCACTACG ACCCTGATGA TGATGAAGAG TACTACAGAA AACAGCTCTC ATTTTTCGAC 
CGCCGCAGTT TCGACAGTAA GGTCATGAAT CCACCCAGCG CTGGAGTAAA CCGCTTCCAT 
GAGCCGCCCA AACCACCCCA GACACAGATC GCTTATCCTT TCGCTAGGAC GGAGTCTGTG 
GAGAAGGTGA GTCCTGTGGA CAAGAGATAC GAGCCTCTGC CGCCAGTCAA CCCTTCACCT 
GCTCCATACA GCCAGAACAC ACCTGTCGCT CCGCCCACAT CCCTGCCCAA ACTCACCAAC 
ATCGAGGTGA ACTCTCTGCC AGATCCTCAC AGCTCTCCCA AAGCCAAACC GGACCTGTCT 
GCTCTGAGAG CTCCGACCCG GGACGAGCCC ATCCAGAGCA GCTACTTGCC CCCCAAGTCC 
ACCATCAATG GCATGGATGC TCCTCCCAAA ACACTCGGCG TGCCGACCAG CTACAACCGC 
TACGTCCCCA AGCCTTACAC CAGCTCGGCG CGACCCTTCG AGCGCAAGTT CGAGAGTCCG 
AAATTCAACC ACAACCTGCT TCCCAACGAC ACGCAGTCCA AACCCAGTGT CAACAACAAC 
CTGAAGCCTC AGATCTCCCC ACAACCCCAG GACACGGACA GCGGCGTCGA CACCTTCACT 
CGCACCATGG ACAACAGGCC AAAATATCAG CACAACAACA TCAACGCCAT TCCCAAAGCC 
GTCCCAGTCA GCCCCAGTGC ACTGGATGAT GATGAAGAAG ATGAGGGACA CACAGTTGTG 
GCCACGGCGC GGGGCATCTT TAACTGTAAT GGCGGGGTGT TGAGCTCTAT AGAGACGGGC 
GTCAGCATCA TTATCCCACA GGGAGCCATT CCTGATAGTG TGGAGCAGGA GATCTACTTC 
AAAGTGTGCC GGGACAACAG CATCCTGCCT CCTCTGGATA AGGAGAAAGG TGAAACTCTG 
CTGAGTCCGC TGGTGATGTG TGGTCCTCAC GGCCTGAAGT TCCTGAAGCC TGTGGAGCTG 
CGCTTGCCAC ACTGTGCGTC TATGACCCCT GATGGTTGGT CTTTTGCTCT AAAATCCTCC 
GACTCCTCGT CGGGTGACCC CAAATGCTGG CAAAACAAGT GTCTACCAGG AGACCCCAAT 
TACCTGGTGG GCGCAAACTG TGTGTCTGTG CTGATCGACC ACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209661.1
CDS	110..6157
Translation	MLLSVFGGPVEEVLAGLLKLKMFGSGSKKWFIKKNKRQRTKVQRKPASGRRNKRVSRVLKRHKEVIVKQLDVSKVLPTLVYERVFTLGEYKEILGQDSSGKRAELFLDLLSQKEPAAFSAFCGVLEEVCPHLLTCFLLDGTDGSAGENKKQGQTKPAHSQQNALSKGQDTVCFPPMYTDPLFDTRVFPKHDKDKYGPAVYYPKFNAYIPNPDKVSVLDESEGSGHGKPSLRRIKGRIHRSKSLDSIDLLDSNSAAMDETVIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGLLQENDRVVMVNAVSMDNVDHAYAVQQLRKSGKNAKITIRRKRKVQIPSGRPGERETMSEHDEDDDSYEDEIYESRSARSGPSAYSGGGGGGGGGGASGRRSGRGDRLSGRRERDRERSGSRERSLSPRSDRHSVSSNLPPRPAKVTLVKSRKNEAEYGLRLASHIFVKDISPESLAARDGNIQEGDVVLKKLIERSKGKLKMVVQRDERATLLNIPDLDDSIPSANASDRDDISDIHSVASDHSNRSHDRKRSSRSRSPDRRSEPSDHSRHSPPQISNGRATGPTAQRTRPVHRSRDEDRISKAGPLPLPAKMVEEMPKEQSAARETEKQLPPLPEPKPVYAQPGQPDVDLPVSPADAPVPSVTHDDSILRPSMKLVKFKKGESVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFANIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHQEVERGIIPNKNRAEQLSSVQYTLPKTPGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVVIFGPIADVAREKLSREETDLFELAKSEPRDAGTDQRSSGIIRLHTIKQIIDRDKHAVLDITPNAVDRLNYAQWYPIVVFLNPDSKQGVKNMRTRLCPESRKSARKLYERALKLRKNNHHLFTTTINLNNMNDGWYGALKESIQQQQNQLVWVSEGKADGAPDDDLDIHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETMNDEVGLPSEPAITRSSEPVREDPPVIQDAAGYPSYQHAVPQPEAVSRIDPAGFKMAAPQQQAEAALASPPPPSAAVQPTATLAGTNPEEPPGTPQADSLNSPVPVPDPQPEPELAQPPTHDPHQSLPPGPDPKMYKKDLYSMDEPVRVTHGVKPPPPQQQQQPLGPPTSLSYSHQPVYQDQQPYRQYDHPPYGYDGGGYAQPKPHNYDPHLHYDNRVPHYNEQWPTYDQQQQQQTSPQPPPASGFPQGHQPPPPPPMGYEPRSPYEDGPNRDFSPPQSQYDGVSPMGYDHRPRHPKPAPVRYEEPPPPPPPVSYDARSPFEPEPHAFPGNTHRSPDPPKQYYGDAAMRPLYNPGPPNRVYKHEPVNSEPPVPPPKPESVLSPGEPPHSAASKPLPPPPRDDDDDDDDDDDPAMKPQSVLNRVKMFENKRSVSVDRAKDAPDVTGIRPTDLPKPASTPGPVLKANSLSNLEQDKPSYRAPEPQKPQTRVGDDVVRSNHYDPDDDEEYYRKQLSFFDRRSFDSKVMNPPSAGVNRFHEPPKPPQTQIAYPFARTESVEKVSPVDKRYEPLPPVNPSPAPYSQNTPVAPPTSLPKLTNIEVNSLPDPHSSPKAKPDLSALRAPTRDEPIQSSYLPPKSTINGMDAPPKTLGVPTSYNRYVPKPYTSSARPFERKFESPKFNHNLLPNDTQSKPSVNNNLKPQISPQPQDTDSGVDTFTRTMDNRPKYQHNNINAIPKAVPVSPSALDDDEEDEGHTVVATARGIFNCNGGVLSSIETGVSIIIPQGAIPDSVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKCWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_017354172.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tight junction protein 1b (tjp1b), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017354172.2

ORF Insert Sequence:

ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
TCCCGCAAGA ATGAAGCAGA ATACGGGCTT CGCCTGGCCA GCCACATCTT CGTGAAGGAC 
ATTTCCCCCG AGAGCCTGGC AGCCCGAGAC GGAAACATCC AAGAGGGAGA CGTGGTGCTT 
AAGAAGCTGA TCGAGAGGTC AAAGGGAAAG CTGAAGATGG TGGTGCAGAG AGACGAGCGG 
GCGACGCTGC TGAACATCCC CGATCTGGAC GACAGCATAC CTTCTGCTAA TGCCTCGGAC 
AGAGATGATA TTTCAGACAT CCACTCCGTG GCCTCTGATC ATTCCAACCG ATCCCACGAC 
CGGAAGAGGA GCAGCCGCTC TCGTTCTCCA GACCGCCGCT CCGAACCCTC AGACCACTCC 
AGACACTCTC CACCACAGAT CAGCAACGGC AGGGCCACAG GGCCGACGGC TCAGAGAACG 
CGGCCAGTTC ACAGGAGCCG TGATGAAGAC AGGATCTCCA AGGCCGGGCC GCTTCCTCTA 
CCTGCTAAGA TGGTGGAGGA GATGCCGAAA GAGCAGAGCG CAGCCAGAGA AACAGAGAAA 
CAGCTCCCCC CTCTGCCAGA GCCCAAACCT GTGTACGCTC AGCCGGGCCA GCCGGACGTG 
GACCTGCCTG TTAGTCCGGC AGATGCTCCT GTGCCCAGCG TCACACATGA TGACAGCATC 
TTACGGCCGA GCATGAAGCT GGTGAAGTTC AAGAAGGGCG AGAGCGTGGG CTTGAGGCTC 
GCTGGGGGGA ACGACGTTGG GATATTTGTG GCGGGAGTTC TGGAGGACAG TCCTGCTGCT 
AAAGAGGGCC TGGAAGAGGG AGACCAGATT CTCAGGGTAA ATAATGTGGA TTTTGCAAAT 
ATCATCCGAG AGGAGGCAGT GCTGTTCCTG CTGGATCTGC CCAAAGGAGA GGAGGTCACC 
ATCCTGGCCC AGAAGAAGAA AGACGTGTAC CGCCGCATTG TAGAGTCTGA CGTGGGAGAC 
TCGTTCTACA TCAGGACTCA TTTTGAATAT GAGAAGGAGT CGCCCTATGG CCTGAGCTTC 
AATAAGGGGG AGGTCTTCAG AGTCGTCGAC ACCCTTTACA ATGGCAAACT GGGCTCCTGG 
CTCGCCATCC GCATCGGCAA AAACCACCAG GAGGTGGAGC GGGGCATCAT CCCCAACAAG 
AACAGGGCTG AGCAGTTATC AAGTGTCCAG TACACTCTCC CAAAGACTCC AGGAGGGGAT 
CGCGCCGACT TCTGGAGATT CAGAGGCCTC CGCAGCTCCA AACGAAACCT GAGGAAGAGC 
AGAGAAGACC TGTCTGCTCA GCCGGTCCAA ACCAAATTCC CTGCTTATGA AAGAGTGGTG 
CTTAGAGAAG CTGGTTTCTT GAGGCCAGTG GTCATTTTCG GACCGATCGC GGATGTGGCT 
AGAGAGAAGC TGTCCAGAGA GGAGACTGAC CTCTTCGAAC TTGCAAAGAG TGAACCCAGA 
GATGCGGGCA CAGACCAGCG CAGCTCCGGA ATCATCCGTC TCCATACCAT TAAACAGATC 
ATTGACCGAG ACAAGCACGC GGTGCTGGAC ATCACCCCTA ATGCGGTGGA CCGTCTGAAC 
TACGCTCAGT GGTACCCCAT CGTGGTGTTT CTGAATCCAG ACAGCAAACA GGGCGTCAAG 
AACATGAGGA CCAGGCTCTG TCCCGAGTCC AGGAAGAGCG CCCGAAAGCT CTACGAGCGT 
GCTCTCAAAC TGAGGAAGAA CAACCATCAC CTTTTCACCA CCACTATTAA CTTGAACAAC 
ATGAATGACG GGTGGTACGG TGCTTTGAAA GAGAGCATCC AGCAGCAGCA GAACCAGTTG 
GTCTGGGTCT CAGAGGGCAA GGCCGACGGC GCTCCTGACG ATGACCTTGA CATCCATGAC 
GACCGCCTCT CCTACCTCTC GGCTCCGGGC AGCGAGTACA GCATGTACAG CACCGACAGC 
CGCCACACGT CCGACTATGA GGACACCGAC ACGGAGGGTG GTGCCTACAC CGACCAGGAG 
CTGGACGAGA CCATGAACGA TGAGGTGGGC CTGCCTAGCG AGCCTGCCAT CACACGCTCA 
TCTGAGCCTG TGCGAGAGGA CCCGCCAGTC ATTCAGGACG CAGCTGGATA CCCCAGCTAC 
CAGCATGCGG TACCGCAACC TGAAGCGGTG AGCCGCATTG ACCCGGCCGG GTTTAAGATG 
GCAGCGCCGC AGCAGCAAGC TGAGGCTGCT CTGGCCTCGC CGCCCCCTCC CTCTGCAGCG 
GTCCAGCCCA CCGCAACACT AGCGGGTACG AACCCCGAGG AGCCGCCTGG CACCCCTCAG 
GCCGACTCCC TTAACAGCCC CGTCCCTGTT CCCGACCCCC AGCCCGAACC TGAGCTCGCT 
CAGCCCCCAA CACACGACCC CCACCAGTCG CTTCCTCCTG GCCCAGATCC AAAGATGTAT 
AAAAAGGATC TGTACAGCAT GGACGAGCCG GTGCGAGTGA CCCACGGCGT GAAGCCTCCC 
CCTCCCCAGC AGCAGCAGCA GCCTTTAGGC CCCCCAACCT CGCTCTCCTA CAGCCACCAG 
CCTGTCTACC AGGACCAACA GCCATACCGC CAGTACGACC ACCCGCCTTA TGGCTATGAC 
GGTGGCGGCT ACGCACAACC AAAGCCTCAC AACTACGACC CGCACCTGCA CTACGACAAC 
CGTGTGCCTC ACTATAACGA GCAGTGGCCC ACGTACGACC AGCAGCAGCA GCAGCAGACT 
TCCCCCCAGC CGCCACCTGC GTCCGGCTTC CCTCAGGGCC ACCAGCCTCC GCCTCCACCA 
CCGATGGGAT ATGAGCCACG CTCCCCATAC GAGGACGGAC CTAACAGGGA TTTCAGCCCT 
CCTCAGTCCC AGTATGACGG TGTGTCCCCA ATGGGCTACG ATCACCGGCC GCGGCACCCC 
AAACCTGCAC CGGTCCGATA TGAAGAGCCT CCACCTCCAC CTCCTCCGGT GTCCTACGAT 
GCACGGTCAC CTTTCGAACC CGAGCCGCAT GCTTTCCCAG GCAACACACA TCGCTCGCCA 
GACCCACCGA AACAGTATTA TGGAGACGCC GCTATGAGGC CCTTGTACAA CCCTGGACCT 
CCGAACAGAG TGTACAAACA TGAGCCGGTG AACTCTGAGC CTCCTGTACC GCCTCCCAAA 
CCTGAATCTG TCCTGTCTCC AGGAGAACCG CCCCATTCTG CAGCTTCCAA ACCATTGCCG 
CCTCCTCCCA GAGATGATGA CGATGATGAT GACGATGATG ATGACCCTGC CATGAAGCCC 
CAGTCTGTGC TCAACAGGGT CAAAATGTTC GAAAACAAAC GCTCTGTGTC GGTAGACCGA 
GCCAAGGACG CACCTGACGT CACTGGAATT AGGCCCACAG ATCTCCCCAA ACCTGCGAGC 
ACCCCTGGAC CTGTGCTCAA AGCCAACTCT CTCAGCAACC TGGAGCAGGA CAAACCCTCC 
TACAGAGCTC CAGAGCCGCA GAAACCTCAG ACGCGTGTTG GGGACGATGT GGTTCGCTCC 
AACCACTACG ACCCTGATGA TGATGAAGAG TACTACAGAA AACAGCTCTC ATTTTTCGAC 
CGCCGCAGTT TCGACAGTAA GGTCATGAAT CCACCCAGCG CTGGAGTAAA CCGCTTCCAT 
GAGCCGCCCA AACCACCCCA GACACAGATC GCTTATCCTT TCGCTAGGAC GGAGTCTGTG 
GAGAAGGTGA GTCCTGTGGA CAAGAGATAC GAGCCTCTGC CGCCAGTCAA CCCTTCACCT 
GCTCCATACA GCCAGAACAC ACCTGTCGCT CCGCCCACAT CCCTGCCCAA ACTCACCAAC 
ATCGAGGTGA ACTCTCTGCC AGATCCTCAC AGCTCTCCCA AAGCCAAACC GGACCTGTCT 
GCTCTGAGAG CTCCGACCCG GGACGAGCCC ATCCAGAGCA GCTACTTGCC CCCCAAGTCC 
ACCATCAATG GCATGGATGC TCCTCCCAAA ACACTCGGCG TGCCGACCAG CTACAACCGC 
TACGTCCCCA AGCCTTACAC CAGCTCGGCG CGACCCTTCG AGCGCAAGTT CGAGAGTCCG 
AAATTCAACC ACAACCTGCT TCCCAACGAC ACGCAGTCCA AACCCAGTGT CAACAACAAC 
CTGAAGCCTC AGATCTCCCC ACAACCCCAG GACACGGACA GCGGCGTCGA CACCTTCACT 
CGCACCATGG ACAACAGGCC AAAATATCAG CACAACAACA TCAACGCCAT TCCCAAAGCC 
GTCCCAGTCA GCCCCAGTGC ACTGGATGAT GATGAAGAAG ATGAGGGACA CACAGTTGTG 
GCCACGGCGC GGGGCATCTT TAACTGTAAT GGCGGGGTGT TGAGCTCTAT AGAGACGGGC 
GTCAGCATCA TTATCCCACA GGGAGCCATT CCTGATAGTG TGGAGCAGGA GATCTACTTC 
AAAGTGTGCC GGGACAACAG CATCCTGCCT CCTCTGGATA AGGAGAAAGG TGAAACTCTG 
CTGAGTCCGC TGGTGATGTG TGGTCCTCAC GGCCTGAAGT TCCTGAAGCC TGTGGAGCTG 
CGCTTGCCAC ACTGTGCGTC TATGACCCCT GATGGTTGGT CTTTTGCTCT AAAATCCTCC 
GACTCCTCGT CGGGTGACCC CAAATGCTGG CAAAACAAGT GTCTACCAGG AGACCCCAAT 
TACCTGGTGG GCGCAAACTG TGTGTCTGTG CTGATCGACC ACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp1b ( XM_017354173.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017209662.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X9, mRNA.

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CloneID	ODa57884
Clone ID Related Accession (Same CDS sequence)	XM_017354173.1
Accession Version	XM_017354173.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6033bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tight junction protein ZO-1 isoform X9
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
TCCCGCAAGA ATGAAGCAGA ATACGGGCTT CGCCTGGCCA GCCACATCTT CGTGAAGGAC 
ATTTCCCCCG AGAGCCTGGC AGCCCGAGAC GGAAACATCC AAGAGGGAGA CGTGGTGCTT 
AAGATTAATG GCACGGTAAC AGAGAATTTG TCCCTGATCG ATGCTAAGAA GCTGATCGAG 
AGGTCAAAGG GAAAGCTGAA GATGGTGGTG CAGAGAGACG AGCGGGCGAC GCTGCTGAAC 
ATCCCCGATC TGGACGACAG CATACCTTCT GCTAATGCCT CGGACAGAGA TGATATTTCA 
GACATCCACT CCGTGGCCTC TGATCATTCC AACCGATCCC ACGACCGGAA GAGGAGCAGC 
CGCTCTCGTT CTCCAGACCG CCGCTCCGAA CCCTCAGACC ACTCCAGACA CTCTCCACCA 
CAGATCAGCA ACGGCAGGGC CACAGGGCCG ACGGCTCAGA GAACGCGGCC AGTTCACAGG 
AGCCGTGATG AAGACAGGAT CTCCAAGGCC GGGCCGCTTC CTCTACCTGC TAAGATGGTG 
GAGGAGATGC CGAAAGAGCA GAGCGCAGCC AGAGAAACAG AGAAACAGCT CCCCCCTCTG 
CCAGAGCCCA AACCTGTGTA CGCTCAGCCG GGCCAGCCGG ACGTGGACCT GCCTGTTAGT 
CCGGCAGATG CTCCTGTGCC CAGCGTCACA CATGATGACA GCATCTTACG GCCGAGCATG 
AAGCTGGTGA AGTTCAAGAA GGGCGAGAGC GTGGGCTTGA GGCTCGCTGG GGGGAACGAC 
GTTGGGATAT TTGTGGCGGG AGTTCTGGAG GACAGTCCTG CTGCTAAAGA GGGCCTGGAA 
GAGGGAGACC AGATTCTCAG GGTAAATAAT GTGGATTTTG CAAATATCAT CCGAGAGGAG 
GCAGTGCTGT TCCTGCTGGA TCTGCCCAAA GGAGAGGAGG TCACCATCCT GGCCCAGAAG 
AAGAAAGACG TGTACCGCCG CATTGTAGAG TCTGACGTGG GAGACTCGTT CTACATCAGG 
ACTCATTTTG AATATGAGAA GGAGTCGCCC TATGGCCTGA GCTTCAATAA GGGGGAGGTC 
TTCAGAGTCG TCGACACCCT TTACAATGGC AAACTGGGCT CCTGGCTCGC CATCCGCATC 
GGCAAAAACC ACCAGGAGGT GGAGCGGGGC ATCATCCCCA ACAAGAACAG GGCTGAGCAG 
TTATCAAGTG TCCAGTACAC TCTCCCAAAG ACTCCAGGAG GGGATCGCGC CGACTTCTGG 
AGATTCAGAG GCCTCCGCAG CTCCAAACGA AACCTGAGGA AGAGCAGAGA AGACCTGTCT 
GCTCAGCCGG TCCAAACCAA ATTCCCTGCT TATGAAAGAG TGGTGCTTAG AGAAGCTGGT 
TTCTTGAGGC CAGTGGTCAT TTTCGGACCG ATCGCGGATG TGGCTAGAGA GAAGCTGTCC 
AGAGAGGAGA CTGACCTCTT CGAACTTGCA AAGAGTGAAC CCAGAGATGC GGGCACAGAC 
CAGCGCAGCT CCGGAATCAT CCGTCTCCAT ACCATTAAAC AGATCATTGA CCGAGACAAG 
CACGCGGTGC TGGACATCAC CCCTAATGCG GTGGACCGTC TGAACTACGC TCAGTGGTAC 
CCCATCGTGG TGTTTCTGAA TCCAGACAGC AAACAGGGCG TCAAGAACAT GAGGACCAGG 
CTCTGTCCCG AGTCCAGGAA GAGCGCCCGA AAGCTCTACG AGCGTGCTCT CAAACTGAGG 
AAGAACAACC ATCACCTTTT CACCACCACT ATTAACTTGA ACAACATGAA TGACGGGTGG 
TACGGTGCTT TGAAAGAGAG CATCCAGCAG CAGCAGAACC AGTTGGTCTG GGTCTCAGAG 
GGCAAGGCCG ACGGCGCTCC TGACGATGAC CTTGACATCC ATGACGACCG CCTCTCCTAC 
CTCTCGGCTC CGGGCAGCGA GTACAGCATG TACAGCACCG ACAGCCGCCA CACGTCCGAC 
TATGAGGACA CCGACACGGA GGGTGGTGCC TACACCGACC AGGAGCTGGA CGAGACCATG 
AACGATGAGG TGGGCCTGCC TAGCGAGCCT GCCATCACAC GCTCATCTGA GCCTGTGCGA 
GAGGACCCGC CAGTCATTCA GGACGCAGCT GGATACCCCA GCTACCAGCA TGCGGTACCG 
CAACCTGAAG CGGTGAGCCG CATTGACCCG GCCGGGTTTA AGATGGCAGC GCCGCAGCAG 
CAAGCTGAGG CTGCTCTGGC CTCGCCGCCC CCTCCCTCTG CAGCGGTCCA GCCCACCGCA 
ACACTAGCGG GTACGAACCC CGAGGAGCCG CCTGGCACCC CTCAGGCCGA CTCCCTTAAC 
AGCCCCGTCC CTGTTCCCGA CCCCCAGCCC GAACCTGAGC TCGCTCAGCC CCCAACACAC 
GACCCCCACC AGTCGCTTCC TCCTGGCCCA GATCCAAAGA TGTATAAAAA GGATCTGTAC 
AGCATGGACG AGCCGGTGCG AGTGACCCAC GGCGTGAAGC CTCCCCCTCC CCAGCAGCAG 
CAGCAGCCTT TAGGCCCCCC AACCTCGCTC TCCTACAGCC ACCAGCCTGT CTACCAGGAC 
CAACAGCCAT ACCGCCAGTA CGACCACCCG CCTTATGGCT ATGACGGTGG CGGCTACGCA 
CAACCAAAGC CTCACAACTA CGACCCGCAC CTGCACTACG ACAACCGTGT GCCTCACTAT 
AACGAGCAGT GGCCCACGTA CGACCAGCAG CAGCAGCAGC AGACTTCCCC CCAGCCGCCA 
CCTGCGTCCG GCTTCCCTCA GGGCCACCAG CCTCCGCCTC CACCACCGAT GGGATATGAG 
CCACGCTCCC CATACGAGGA CGGACCTAAC AGGGATTTCA GCCCTCCTCA GTCCCAGTAT 
GACGGTGTGT CCCCAATGGG CTACGATCAC CGGCCGCGGC ACCCCAAACC TGCACCGGTC 
CGATATGAAG AGCCTCCACC TCCACCTCCT CCGGTGTCCT ACGATGCACG GTCACCTTTC 
GAACCCGAGC CGCATGCTTT CCCAGGCAAC ACACATCGCT CGCCAGACCC ACCGAAACAG 
TATTATGGAG ACGCCGCTAT GAGGCCCTTG TACAACCCTG GACCTCCGAA CAGAGTGTAC 
AAACATGAGC CGGTGAACTC TGAGCCTCCT GTACCGCCTC CCAAACCTGA ATCTGTCCTG 
TCTCCAGGAG AACCGCCCCA TTCTGCAGCT TCCAAACCAT TGCCGCCTCC TCCCAGAGAT 
GATGACGATG ATGATGACGA TGATGATGAC CCTGCCATGA AGCCCCAGTC TGTGCTCAAC 
AGGGTCAAAA TGTTCGAAAA CAAACGCTCT GTGTCGGTAG ACCGAGCCAA GGACGCACCT 
GACGTCACTG GAATTAGGCC CACAGATCTC CCCAAACCTG CGAGCACCCC TGGACCTGTG 
CTCAAAGCCA ACTCTCTCAG CAACCTGGAG CAGGACAAAC CCTCCTACAG AGCTCCAGAG 
CCGCAGAAAC CTCAGACGCG TGTTGGGGAC GATGTGGTTC GCTCCAACCA CTACGACCCT 
GATGATGATG AAGAGTACTA CAGAAAACAG CTCTCATTTT TCGACCGCCG CAGTTTCGAC 
AGTAAGGTCA TGAATCCACC CAGCGCTGGA GTAAACCGCT TCCATGAGCC GCCCAAACCA 
CCCCAGACAC AGATCGCTTA TCCTTTCGCT AGGACGGAGT CTGTGGAGAA GGTGAGTCCT 
GTGGACAAGA GATACGAGCC TCTGCCGCCA GTCAACCCTT CACCTGCTCC ATACAGCCAG 
AACACACCTG TCGCTCCGCC CACATCCCTG CCCAAACTCA CCAACATCGA GGTGAACTCT 
CTGCCAGATC CTCACAGCTC TCCCAAAGCC AAACCGGACC TGTCTGCTCT GAGAGCTCCG 
ACCCGGGACG AGCCCATCCA GAGCAGCTAC TTGCCCCCCA AGTCCACCAT CAATGGCATG 
GATGCTCCTC CCAAAACACT CGGCGTGCCG ACCAGCTACA ACCGCTACGT CCCCAAGCCT 
TACACCAGCT CGGCGCGACC CTTCGAGCGC AAGTTCGAGA GTCCGAAATT CAACCACAAC 
CTGCTTCCCA ACGACACGCA GTCCAAACCC AGTGTCAACA ACAACCTGAA GCCTCAGATC 
TCCCCACAAC CCCAGGACAC GGACAGCGGC GTCGACACCT TCACTCGCAC CATGGACAAC 
AGGCCAAAAT ATCAGCACAA CAACATCAAC GCCATTCCCA AAGCCGTCCC AGTCAGCCCC 
AGTGCACTGG ATGATGATGA AGAAGATGAG GGACACACAG TTGTGGCCAC GGCGCGGGGC 
ATCTTTAACT GTAATGGCGG GGTGTTGAGC TCTATAGAGA CGGGCGTCAG CATCATTATC 
CCACAGGGAG CCATTCCTGA TAGTGTGGAG CAGGAGATCT ACTTCAAAGT GTGCCGGGAC 
AACAGCATCC TGCCTCCTCT GGATAAGGAG AAAGGTGAAA CTCTGCTGAG TCCGCTGGTG 
ATGTGTGGTC CTCACGGCCT GAAGTTCCTG AAGCCTGTGG AGCTGCGCTT GCCACACTGT 
GACCCCAAAT GCTGGCAAAA CAAGTGTCTA CCAGGAGACC CCAATTACCT GGTGGGCGCA 
AACTGTGTGT CTGTGCTGAT CGACCACTTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209662.1
CDS	115..6147
Translation	MLLSVFGGPVEEVLAGLLKLKMFGSGSKKWFIKKNKRQRTKVQRKPASGRRNKRVSRVLKRHKEVIVKQLDVSKVLPTLVYERVFTLGEYKEILGQDSSGKRAELFLDLLSQKEPAAFSAFCGVLEEVCPHLLTCFLLDGTDGSAGENKKQGQTKPAHSQQNALSKGQDTVCFPPMYTDPLFDTRVFPKHDKDKYGPAVYYPKFNAYIPNPDKVSVLDESEGSGHGKPSLRRIKGRIHRSKSLDSIDLLDSNSAAMDETVIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGLLQENDRVVMVNAVSMDNVDHAYAVQQLRKSGKNAKITIRRKRKVQIPSGRPGERETMSEHDEDDDSYEDEIYESRSARSGPSAYSGGGGGGGGGGASGRRSGRGDRLSGRRERDRERSGSRERSLSPRSDRHSVSSNLPPRPAKVTLVKSRKNEAEYGLRLASHIFVKDISPESLAARDGNIQEGDVVLKINGTVTENLSLIDAKKLIERSKGKLKMVVQRDERATLLNIPDLDDSIPSANASDRDDISDIHSVASDHSNRSHDRKRSSRSRSPDRRSEPSDHSRHSPPQISNGRATGPTAQRTRPVHRSRDEDRISKAGPLPLPAKMVEEMPKEQSAARETEKQLPPLPEPKPVYAQPGQPDVDLPVSPADAPVPSVTHDDSILRPSMKLVKFKKGESVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFANIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHQEVERGIIPNKNRAEQLSSVQYTLPKTPGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVVIFGPIADVAREKLSREETDLFELAKSEPRDAGTDQRSSGIIRLHTIKQIIDRDKHAVLDITPNAVDRLNYAQWYPIVVFLNPDSKQGVKNMRTRLCPESRKSARKLYERALKLRKNNHHLFTTTINLNNMNDGWYGALKESIQQQQNQLVWVSEGKADGAPDDDLDIHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETMNDEVGLPSEPAITRSSEPVREDPPVIQDAAGYPSYQHAVPQPEAVSRIDPAGFKMAAPQQQAEAALASPPPPSAAVQPTATLAGTNPEEPPGTPQADSLNSPVPVPDPQPEPELAQPPTHDPHQSLPPGPDPKMYKKDLYSMDEPVRVTHGVKPPPPQQQQQPLGPPTSLSYSHQPVYQDQQPYRQYDHPPYGYDGGGYAQPKPHNYDPHLHYDNRVPHYNEQWPTYDQQQQQQTSPQPPPASGFPQGHQPPPPPPMGYEPRSPYEDGPNRDFSPPQSQYDGVSPMGYDHRPRHPKPAPVRYEEPPPPPPPVSYDARSPFEPEPHAFPGNTHRSPDPPKQYYGDAAMRPLYNPGPPNRVYKHEPVNSEPPVPPPKPESVLSPGEPPHSAASKPLPPPPRDDDDDDDDDDDPAMKPQSVLNRVKMFENKRSVSVDRAKDAPDVTGIRPTDLPKPASTPGPVLKANSLSNLEQDKPSYRAPEPQKPQTRVGDDVVRSNHYDPDDDEEYYRKQLSFFDRRSFDSKVMNPPSAGVNRFHEPPKPPQTQIAYPFARTESVEKVSPVDKRYEPLPPVNPSPAPYSQNTPVAPPTSLPKLTNIEVNSLPDPHSSPKAKPDLSALRAPTRDEPIQSSYLPPKSTINGMDAPPKTLGVPTSYNRYVPKPYTSSARPFERKFESPKFNHNLLPNDTQSKPSVNNNLKPQISPQPQDTDSGVDTFTRTMDNRPKYQHNNINAIPKAVPVSPSALDDDEEDEGHTVVATARGIFNCNGGVLSSIETGVSIIIPQGAIPDSVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCDPKCWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_017354173.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tight junction protein 1b (tjp1b), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017354173.1

ORF Insert Sequence:

ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
TCCCGCAAGA ATGAAGCAGA ATACGGGCTT CGCCTGGCCA GCCACATCTT CGTGAAGGAC 
ATTTCCCCCG AGAGCCTGGC AGCCCGAGAC GGAAACATCC AAGAGGGAGA CGTGGTGCTT 
AAGATTAATG GCACGGTAAC AGAGAATTTG TCCCTGATCG ATGCTAAGAA GCTGATCGAG 
AGGTCAAAGG GAAAGCTGAA GATGGTGGTG CAGAGAGACG AGCGGGCGAC GCTGCTGAAC 
ATCCCCGATC TGGACGACAG CATACCTTCT GCTAATGCCT CGGACAGAGA TGATATTTCA 
GACATCCACT CCGTGGCCTC TGATCATTCC AACCGATCCC ACGACCGGAA GAGGAGCAGC 
CGCTCTCGTT CTCCAGACCG CCGCTCCGAA CCCTCAGACC ACTCCAGACA CTCTCCACCA 
CAGATCAGCA ACGGCAGGGC CACAGGGCCG ACGGCTCAGA GAACGCGGCC AGTTCACAGG 
AGCCGTGATG AAGACAGGAT CTCCAAGGCC GGGCCGCTTC CTCTACCTGC TAAGATGGTG 
GAGGAGATGC CGAAAGAGCA GAGCGCAGCC AGAGAAACAG AGAAACAGCT CCCCCCTCTG 
CCAGAGCCCA AACCTGTGTA CGCTCAGCCG GGCCAGCCGG ACGTGGACCT GCCTGTTAGT 
CCGGCAGATG CTCCTGTGCC CAGCGTCACA CATGATGACA GCATCTTACG GCCGAGCATG 
AAGCTGGTGA AGTTCAAGAA GGGCGAGAGC GTGGGCTTGA GGCTCGCTGG GGGGAACGAC 
GTTGGGATAT TTGTGGCGGG AGTTCTGGAG GACAGTCCTG CTGCTAAAGA GGGCCTGGAA 
GAGGGAGACC AGATTCTCAG GGTAAATAAT GTGGATTTTG CAAATATCAT CCGAGAGGAG 
GCAGTGCTGT TCCTGCTGGA TCTGCCCAAA GGAGAGGAGG TCACCATCCT GGCCCAGAAG 
AAGAAAGACG TGTACCGCCG CATTGTAGAG TCTGACGTGG GAGACTCGTT CTACATCAGG 
ACTCATTTTG AATATGAGAA GGAGTCGCCC TATGGCCTGA GCTTCAATAA GGGGGAGGTC 
TTCAGAGTCG TCGACACCCT TTACAATGGC AAACTGGGCT CCTGGCTCGC CATCCGCATC 
GGCAAAAACC ACCAGGAGGT GGAGCGGGGC ATCATCCCCA ACAAGAACAG GGCTGAGCAG 
TTATCAAGTG TCCAGTACAC TCTCCCAAAG ACTCCAGGAG GGGATCGCGC CGACTTCTGG 
AGATTCAGAG GCCTCCGCAG CTCCAAACGA AACCTGAGGA AGAGCAGAGA AGACCTGTCT 
GCTCAGCCGG TCCAAACCAA ATTCCCTGCT TATGAAAGAG TGGTGCTTAG AGAAGCTGGT 
TTCTTGAGGC CAGTGGTCAT TTTCGGACCG ATCGCGGATG TGGCTAGAGA GAAGCTGTCC 
AGAGAGGAGA CTGACCTCTT CGAACTTGCA AAGAGTGAAC CCAGAGATGC GGGCACAGAC 
CAGCGCAGCT CCGGAATCAT CCGTCTCCAT ACCATTAAAC AGATCATTGA CCGAGACAAG 
CACGCGGTGC TGGACATCAC CCCTAATGCG GTGGACCGTC TGAACTACGC TCAGTGGTAC 
CCCATCGTGG TGTTTCTGAA TCCAGACAGC AAACAGGGCG TCAAGAACAT GAGGACCAGG 
CTCTGTCCCG AGTCCAGGAA GAGCGCCCGA AAGCTCTACG AGCGTGCTCT CAAACTGAGG 
AAGAACAACC ATCACCTTTT CACCACCACT ATTAACTTGA ACAACATGAA TGACGGGTGG 
TACGGTGCTT TGAAAGAGAG CATCCAGCAG CAGCAGAACC AGTTGGTCTG GGTCTCAGAG 
GGCAAGGCCG ACGGCGCTCC TGACGATGAC CTTGACATCC ATGACGACCG CCTCTCCTAC 
CTCTCGGCTC CGGGCAGCGA GTACAGCATG TACAGCACCG ACAGCCGCCA CACGTCCGAC 
TATGAGGACA CCGACACGGA GGGTGGTGCC TACACCGACC AGGAGCTGGA CGAGACCATG 
AACGATGAGG TGGGCCTGCC TAGCGAGCCT GCCATCACAC GCTCATCTGA GCCTGTGCGA 
GAGGACCCGC CAGTCATTCA GGACGCAGCT GGATACCCCA GCTACCAGCA TGCGGTACCG 
CAACCTGAAG CGGTGAGCCG CATTGACCCG GCCGGGTTTA AGATGGCAGC GCCGCAGCAG 
CAAGCTGAGG CTGCTCTGGC CTCGCCGCCC CCTCCCTCTG CAGCGGTCCA GCCCACCGCA 
ACACTAGCGG GTACGAACCC CGAGGAGCCG CCTGGCACCC CTCAGGCCGA CTCCCTTAAC 
AGCCCCGTCC CTGTTCCCGA CCCCCAGCCC GAACCTGAGC TCGCTCAGCC CCCAACACAC 
GACCCCCACC AGTCGCTTCC TCCTGGCCCA GATCCAAAGA TGTATAAAAA GGATCTGTAC 
AGCATGGACG AGCCGGTGCG AGTGACCCAC GGCGTGAAGC CTCCCCCTCC CCAGCAGCAG 
CAGCAGCCTT TAGGCCCCCC AACCTCGCTC TCCTACAGCC ACCAGCCTGT CTACCAGGAC 
CAACAGCCAT ACCGCCAGTA CGACCACCCG CCTTATGGCT ATGACGGTGG CGGCTACGCA 
CAACCAAAGC CTCACAACTA CGACCCGCAC CTGCACTACG ACAACCGTGT GCCTCACTAT 
AACGAGCAGT GGCCCACGTA CGACCAGCAG CAGCAGCAGC AGACTTCCCC CCAGCCGCCA 
CCTGCGTCCG GCTTCCCTCA GGGCCACCAG CCTCCGCCTC CACCACCGAT GGGATATGAG 
CCACGCTCCC CATACGAGGA CGGACCTAAC AGGGATTTCA GCCCTCCTCA GTCCCAGTAT 
GACGGTGTGT CCCCAATGGG CTACGATCAC CGGCCGCGGC ACCCCAAACC TGCACCGGTC 
CGATATGAAG AGCCTCCACC TCCACCTCCT CCGGTGTCCT ACGATGCACG GTCACCTTTC 
GAACCCGAGC CGCATGCTTT CCCAGGCAAC ACACATCGCT CGCCAGACCC ACCGAAACAG 
TATTATGGAG ACGCCGCTAT GAGGCCCTTG TACAACCCTG GACCTCCGAA CAGAGTGTAC 
AAACATGAGC CGGTGAACTC TGAGCCTCCT GTACCGCCTC CCAAACCTGA ATCTGTCCTG 
TCTCCAGGAG AACCGCCCCA TTCTGCAGCT TCCAAACCAT TGCCGCCTCC TCCCAGAGAT 
GATGACGATG ATGATGACGA TGATGATGAC CCTGCCATGA AGCCCCAGTC TGTGCTCAAC 
AGGGTCAAAA TGTTCGAAAA CAAACGCTCT GTGTCGGTAG ACCGAGCCAA GGACGCACCT 
GACGTCACTG GAATTAGGCC CACAGATCTC CCCAAACCTG CGAGCACCCC TGGACCTGTG 
CTCAAAGCCA ACTCTCTCAG CAACCTGGAG CAGGACAAAC CCTCCTACAG AGCTCCAGAG 
CCGCAGAAAC CTCAGACGCG TGTTGGGGAC GATGTGGTTC GCTCCAACCA CTACGACCCT 
GATGATGATG AAGAGTACTA CAGAAAACAG CTCTCATTTT TCGACCGCCG CAGTTTCGAC 
AGTAAGGTCA TGAATCCACC CAGCGCTGGA GTAAACCGCT TCCATGAGCC GCCCAAACCA 
CCCCAGACAC AGATCGCTTA TCCTTTCGCT AGGACGGAGT CTGTGGAGAA GGTGAGTCCT 
GTGGACAAGA GATACGAGCC TCTGCCGCCA GTCAACCCTT CACCTGCTCC ATACAGCCAG 
AACACACCTG TCGCTCCGCC CACATCCCTG CCCAAACTCA CCAACATCGA GGTGAACTCT 
CTGCCAGATC CTCACAGCTC TCCCAAAGCC AAACCGGACC TGTCTGCTCT GAGAGCTCCG 
ACCCGGGACG AGCCCATCCA GAGCAGCTAC TTGCCCCCCA AGTCCACCAT CAATGGCATG 
GATGCTCCTC CCAAAACACT CGGCGTGCCG ACCAGCTACA ACCGCTACGT CCCCAAGCCT 
TACACCAGCT CGGCGCGACC CTTCGAGCGC AAGTTCGAGA GTCCGAAATT CAACCACAAC 
CTGCTTCCCA ACGACACGCA GTCCAAACCC AGTGTCAACA ACAACCTGAA GCCTCAGATC 
TCCCCACAAC CCCAGGACAC GGACAGCGGC GTCGACACCT TCACTCGCAC CATGGACAAC 
AGGCCAAAAT ATCAGCACAA CAACATCAAC GCCATTCCCA AAGCCGTCCC AGTCAGCCCC 
AGTGCACTGG ATGATGATGA AGAAGATGAG GGACACACAG TTGTGGCCAC GGCGCGGGGC 
ATCTTTAACT GTAATGGCGG GGTGTTGAGC TCTATAGAGA CGGGCGTCAG CATCATTATC 
CCACAGGGAG CCATTCCTGA TAGTGTGGAG CAGGAGATCT ACTTCAAAGT GTGCCGGGAC 
AACAGCATCC TGCCTCCTCT GGATAAGGAG AAAGGTGAAA CTCTGCTGAG TCCGCTGGTG 
ATGTGTGGTC CTCACGGCCT GAAGTTCCTG AAGCCTGTGG AGCTGCGCTT GCCACACTGT 
GACCCCAAAT GCTGGCAAAA CAAGTGTCTA CCAGGAGACC CCAATTACCT GGTGGGCGCA 
AACTGTGTGT CTGTGCTGAT CGACCACTTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp1b ( XM_017354175.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017209664.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X10, mRNA.

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CloneID	ODa57885
Clone ID Related Accession (Same CDS sequence)	XM_017354175.1
Accession Version	XM_017354175.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5874bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tight junction protein ZO-1 isoform X10
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
TCCCGCAAGA ATGAAGCAGA ATACGGGCTT CGCCTGGCCA GCCACATCTT CGTGAAGGAC 
ATTTCCCCCG AGAGCCTGGC AGCCCGAGAC GGAAACATCC AAGAGGGAGA CGTGGTGCTT 
AAGATTAATG GCACGGTAAC AGAGAATTTG TCCCTGATCG ATGCTAAGAA GCTGATCGAG 
AGGTCAAAGG GAAAGCTGAA GATGGTGGTG CAGAGAGACG AGCGGGCGAC GCTGCTGAAC 
ATCCCCGATC TGGACGACAG CATACCTTCT GCTAATGCCT CGGACAGAGA TGATATTTCA 
GACATCCACT CCGTGGCCTC TGATCATTCC AACCGATCCC ACGACCGGAA GAGGAGCAGC 
CGCTCTCGTT CTCCAGACCG CCGCTCCGAA CCCTCAGACC ACTCCAGACA CTCTCCACCA 
CAGATCAGCA ACGGCAGGGC CACAGGGCCG ACGGCTCAGA GAACGCGGCC AGTTCACAGG 
AGCCGTGATG AAGACAGGAT CTCCAAGGCC GGGCCGCTTC CTCTACCTGC TAAGATGGTG 
GAGGAGATGC CGAAAGAGCA GAGCGCAGCC AGAGAAACAG AGAAACAGCT CCCCCCTCTG 
CCAGAGCCCA AACCTGTGTA CGCTCAGCCG GGCCAGCCGG ACGTGGACCT GCCTGTTAGT 
CCGGCAGATG CTCCTGTGCC CAGCGTCACA CATGATGACA GCATCTTACG GCCGAGCATG 
AAGCTGGTGA AGTTCAAGAA GGGCGAGAGC GTGGGCTTGA GGCTCGCTGG GGGGAACGAC 
GTTGGGATAT TTGTGGCGGG AGTTCTGGAG GACAGTCCTG CTGCTAAAGA GGGCCTGGAA 
GAGGGAGACC AGATTCTCAG GGTAAATAAT GTGGATTTTG CAAATATCAT CCGAGAGGAG 
GCAGTGCTGT TCCTGCTGGA TCTGCCCAAA GGAGAGGAGG TCACCATCCT GGCCCAGAAG 
AAGAAAGACG TGTACCGCCG CATTGTAGAG TCTGACGTGG GAGACTCGTT CTACATCAGG 
ACTCATTTTG AATATGAGAA GGAGTCGCCC TATGGCCTGA GCTTCAATAA GGGGGAGGTC 
TTCAGAGTCG TCGACACCCT TTACAATGGC AAACTGGGCT CCTGGCTCGC CATCCGCATC 
GGCAAAAACC ACCAGGAGGT GGAGCGGGGC ATCATCCCCA ACAAGAACAG GGCTGAGCAG 
TTATCAAGTG TCCAGTACAC TCTCCCAAAG ACTCCAGGAG GGGATCGCGC CGACTTCTGG 
AGATTCAGAG GCCTCCGCAG CTCCAAACGA AACCTGAGGA AGAGCAGAGA AGACCTGTCT 
GCTCAGCCGG TCCAAACCAA ATTCCCTGCT TATGAAAGAG TGGTGCTTAG AGAAGCTGGT 
TTCTTGAGGC CAGTGGTCAT TTTCGGACCG ATCGCGGATG TGGCTAGAGA GAAGCTGTCC 
AGAGAGGAGA CTGACCTCTT CGAACTTGCA AAGAGTGAAC CCAGAGATGC GGGCACAGAC 
CAGCGCAGCT CCGGAATCAT CCGTCTCCAT ACCATTAAAC AGATCATTGA CCGAGACAAG 
CACGCGGTGC TGGACATCAC CCCTAATGCG GTGGACCGTC TGAACTACGC TCAGTGGTAC 
CCCATCGTGG TGTTTCTGAA TCCAGACAGC AAACAGGGCG TCAAGAACAT GAGGACCAGG 
CTCTGTCCCG AGTCCAGGAA GAGCGCCCGA AAGCTCTACG AGCGTGCTCT CAAACTGAGG 
AAGAACAACC ATCACCTTTT CACCACCACT ATTAACTTGA ACAACATGAA TGACGGGTGG 
TACGGTGCTT TGAAAGAGAG CATCCAGCAG CAGCAGAACC AGTTGGTCTG GGTCTCAGAG 
GGCAAGGCCG ACGGCGCTCC TGACGATGAC CTTGACATCC ATGACGACCG CCTCTCCTAC 
CTCTCGGCTC CGGGCAGCGA GTACAGCATG TACAGCACCG ACAGCCGCCA CACGTCCGAC 
TATGAGGACA CCGACACGGA GGGTGGTGCC TACACCGACC AGGAGCTGGA CGAGACCATG 
AACGATGAGG TGGGCCTGCC TAGCGAGCCT GCCATCACAC GCTCATCTGA GCCTGTGCGA 
GAGGACCCGC CAGTCATTCA GGACGCAGCT GGATACCCCA GCTACCAGCA TGCGGTACCG 
CAACCTGAAG CGGTGAGCCG CATTGACCCG GCCGGGTTTA AGATGGCAGC GCCGCAGCAG 
ATGTATAAAA AGGATCTGTA CAGCATGGAC GAGCCGGTGC GAGTGACCCA CGGCGTGAAG 
CCTCCCCCTC CCCAGCAGCA GCAGCAGCCT TTAGGCCCCC CAACCTCGCT CTCCTACAGC 
CACCAGCCTG TCTACCAGGA CCAACAGCCA TACCGCCAGT ACGACCACCC GCCTTATGGC 
TATGACGGTG GCGGCTACGC ACAACCAAAG CCTCACAACT ACGACCCGCA CCTGCACTAC 
GACAACCGTG TGCCTCACTA TAACGAGCAG TGGCCCACGT ACGACCAGCA GCAGCAGCAG 
CAGACTTCCC CCCAGCCGCC ACCTGCGTCC GGCTTCCCTC AGGGCCACCA GCCTCCGCCT 
CCACCACCGA TGGGATATGA GCCACGCTCC CCATACGAGG ACGGACCTAA CAGGGATTTC 
AGCCCTCCTC AGTCCCAGTA TGACGGTGTG TCCCCAATGG GCTACGATCA CCGGCCGCGG 
CACCCCAAAC CTGCACCGGT CCGATATGAA GAGCCTCCAC CTCCACCTCC TCCGGTGTCC 
TACGATGCAC GGTCACCTTT CGAACCCGAG CCGCATGCTT TCCCAGGCAA CACACATCGC 
TCGCCAGACC CACCGAAACA GTATTATGGA GACGCCGCTA TGAGGCCCTT GTACAACCCT 
GGACCTCCGA ACAGAGTGTA CAAACATGAG CCGGTGAACT CTGAGCCTCC TGTACCGCCT 
CCCAAACCTG AATCTGTCCT GTCTCCAGGA GAACCGCCCC ATTCTGCAGC TTCCAAACCA 
TTGCCGCCTC CTCCCAGAGA TGATGACGAT GATGATGACG ATGATGATGA CCCTGCCATG 
AAGCCCCAGT CTGTGCTCAA CAGGGTCAAA ATGTTCGAAA ACAAACGCTC TGTGTCGGTA 
GACCGAGCCA AGGACGCACC TGACGTCACT GGAATTAGGC CCACAGATCT CCCCAAACCT 
GCGAGCACCC CTGGACCTGT GCTCAAAGCC AACTCTCTCA GCAACCTGGA GCAGGACAAA 
CCCTCCTACA GAGCTCCAGA GCCGCAGAAA CCTCAGACGC GTGTTGGGGA CGATGTGGTT 
CGCTCCAACC ACTACGACCC TGATGATGAT GAAGAGTACT ACAGAAAACA GCTCTCATTT 
TTCGACCGCC GCAGTTTCGA CAGTAAGGTC ATGAATCCAC CCAGCGCTGG AGTAAACCGC 
TTCCATGAGC CGCCCAAACC ACCCCAGACA CAGATCGCTT ATCCTTTCGC TAGGACGGAG 
TCTGTGGAGA AGGTGAGTCC TGTGGACAAG AGATACGAGC CTCTGCCGCC AGTCAACCCT 
TCACCTGCTC CATACAGCCA GAACACACCT GTCGCTCCGC CCACATCCCT GCCCAAACTC 
ACCAACATCG AGGTGAACTC TCTGCCAGAT CCTCACAGCT CTCCCAAAGC CAAACCGGAC 
CTGTCTGCTC TGAGAGCTCC GACCCGGGAC GAGCCCATCC AGAGCAGCTA CTTGCCCCCC 
AAGTCCACCA TCAATGGCAT GGATGCTCCT CCCAAAACAC TCGGCGTGCC GACCAGCTAC 
AACCGCTACG TCCCCAAGCC TTACACCAGC TCGGCGCGAC CCTTCGAGCG CAAGTTCGAG 
AGTCCGAAAT TCAACCACAA CCTGCTTCCC AACGACACGC AGTCCAAACC CAGTGTCAAC 
AACAACCTGA AGCCTCAGAT CTCCCCACAA CCCCAGGACA CGGACAGCGG CGTCGACACC 
TTCACTCGCA CCATGGACAA CAGGCCAAAA TATCAGCACA ACAACATCAA CGCCATTCCC 
AAAGCCGTCC CAGTCAGCCC CAGTGCACTG GATGATGATG AAGAAGATGA GGGACACACA 
GTTGTGGCCA CGGCGCGGGG CATCTTTAAC TGTAATGGCG GGGTGTTGAG CTCTATAGAG 
ACGGGCGTCA GCATCATTAT CCCACAGGGA GCCATTCCTG ATAGTGTGGA GCAGGAGATC 
TACTTCAAAG TGTGCCGGGA CAACAGCATC CTGCCTCCTC TGGATAAGGA GAAAGGTGAA 
ACTCTGCTGA GTCCGCTGGT GATGTGTGGT CCTCACGGCC TGAAGTTCCT GAAGCCTGTG 
GAGCTGCGCT TGCCACACTG TGCGTCTATG ACCCCTGATG GTTGGTCTTT TGCTCTAAAA 
TCCTCCGACT CCTCGTCGGG TGACCCCAAA TGCTGGCAAA ACAAGTGTCT ACCAGGAGAC 
CCCAATTACC TGGTGGGCGC AAACTGTGTG TCTGTGCTGA TCGACCACTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209664.1
CDS	114..5987
Translation	MLLSVFGGPVEEVLAGLLKLKMFGSGSKKWFIKKNKRQRTKVQRKPASGRRNKRVSRVLKRHKEVIVKQLDVSKVLPTLVYERVFTLGEYKEILGQDSSGKRAELFLDLLSQKEPAAFSAFCGVLEEVCPHLLTCFLLDGTDGSAGENKKQGQTKPAHSQQNALSKGQDTVCFPPMYTDPLFDTRVFPKHDKDKYGPAVYYPKFNAYIPNPDKVSVLDESEGSGHGKPSLRRIKGRIHRSKSLDSIDLLDSNSAAMDETVIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGLLQENDRVVMVNAVSMDNVDHAYAVQQLRKSGKNAKITIRRKRKVQIPSGRPGERETMSEHDEDDDSYEDEIYESRSARSGPSAYSGGGGGGGGGGASGRRSGRGDRLSGRRERDRERSGSRERSLSPRSDRHSVSSNLPPRPAKVTLVKSRKNEAEYGLRLASHIFVKDISPESLAARDGNIQEGDVVLKINGTVTENLSLIDAKKLIERSKGKLKMVVQRDERATLLNIPDLDDSIPSANASDRDDISDIHSVASDHSNRSHDRKRSSRSRSPDRRSEPSDHSRHSPPQISNGRATGPTAQRTRPVHRSRDEDRISKAGPLPLPAKMVEEMPKEQSAARETEKQLPPLPEPKPVYAQPGQPDVDLPVSPADAPVPSVTHDDSILRPSMKLVKFKKGESVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFANIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHQEVERGIIPNKNRAEQLSSVQYTLPKTPGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVVIFGPIADVAREKLSREETDLFELAKSEPRDAGTDQRSSGIIRLHTIKQIIDRDKHAVLDITPNAVDRLNYAQWYPIVVFLNPDSKQGVKNMRTRLCPESRKSARKLYERALKLRKNNHHLFTTTINLNNMNDGWYGALKESIQQQQNQLVWVSEGKADGAPDDDLDIHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETMNDEVGLPSEPAITRSSEPVREDPPVIQDAAGYPSYQHAVPQPEAVSRIDPAGFKMAAPQQMYKKDLYSMDEPVRVTHGVKPPPPQQQQQPLGPPTSLSYSHQPVYQDQQPYRQYDHPPYGYDGGGYAQPKPHNYDPHLHYDNRVPHYNEQWPTYDQQQQQQTSPQPPPASGFPQGHQPPPPPPMGYEPRSPYEDGPNRDFSPPQSQYDGVSPMGYDHRPRHPKPAPVRYEEPPPPPPPVSYDARSPFEPEPHAFPGNTHRSPDPPKQYYGDAAMRPLYNPGPPNRVYKHEPVNSEPPVPPPKPESVLSPGEPPHSAASKPLPPPPRDDDDDDDDDDDPAMKPQSVLNRVKMFENKRSVSVDRAKDAPDVTGIRPTDLPKPASTPGPVLKANSLSNLEQDKPSYRAPEPQKPQTRVGDDVVRSNHYDPDDDEEYYRKQLSFFDRRSFDSKVMNPPSAGVNRFHEPPKPPQTQIAYPFARTESVEKVSPVDKRYEPLPPVNPSPAPYSQNTPVAPPTSLPKLTNIEVNSLPDPHSSPKAKPDLSALRAPTRDEPIQSSYLPPKSTINGMDAPPKTLGVPTSYNRYVPKPYTSSARPFERKFESPKFNHNLLPNDTQSKPSVNNNLKPQISPQPQDTDSGVDTFTRTMDNRPKYQHNNINAIPKAVPVSPSALDDDEEDEGHTVVATARGIFNCNGGVLSSIETGVSIIIPQGAIPDSVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKCWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_017354175.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tight junction protein 1b (tjp1b), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017354175.1

ORF Insert Sequence:

ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
TCCCGCAAGA ATGAAGCAGA ATACGGGCTT CGCCTGGCCA GCCACATCTT CGTGAAGGAC 
ATTTCCCCCG AGAGCCTGGC AGCCCGAGAC GGAAACATCC AAGAGGGAGA CGTGGTGCTT 
AAGATTAATG GCACGGTAAC AGAGAATTTG TCCCTGATCG ATGCTAAGAA GCTGATCGAG 
AGGTCAAAGG GAAAGCTGAA GATGGTGGTG CAGAGAGACG AGCGGGCGAC GCTGCTGAAC 
ATCCCCGATC TGGACGACAG CATACCTTCT GCTAATGCCT CGGACAGAGA TGATATTTCA 
GACATCCACT CCGTGGCCTC TGATCATTCC AACCGATCCC ACGACCGGAA GAGGAGCAGC 
CGCTCTCGTT CTCCAGACCG CCGCTCCGAA CCCTCAGACC ACTCCAGACA CTCTCCACCA 
CAGATCAGCA ACGGCAGGGC CACAGGGCCG ACGGCTCAGA GAACGCGGCC AGTTCACAGG 
AGCCGTGATG AAGACAGGAT CTCCAAGGCC GGGCCGCTTC CTCTACCTGC TAAGATGGTG 
GAGGAGATGC CGAAAGAGCA GAGCGCAGCC AGAGAAACAG AGAAACAGCT CCCCCCTCTG 
CCAGAGCCCA AACCTGTGTA CGCTCAGCCG GGCCAGCCGG ACGTGGACCT GCCTGTTAGT 
CCGGCAGATG CTCCTGTGCC CAGCGTCACA CATGATGACA GCATCTTACG GCCGAGCATG 
AAGCTGGTGA AGTTCAAGAA GGGCGAGAGC GTGGGCTTGA GGCTCGCTGG GGGGAACGAC 
GTTGGGATAT TTGTGGCGGG AGTTCTGGAG GACAGTCCTG CTGCTAAAGA GGGCCTGGAA 
GAGGGAGACC AGATTCTCAG GGTAAATAAT GTGGATTTTG CAAATATCAT CCGAGAGGAG 
GCAGTGCTGT TCCTGCTGGA TCTGCCCAAA GGAGAGGAGG TCACCATCCT GGCCCAGAAG 
AAGAAAGACG TGTACCGCCG CATTGTAGAG TCTGACGTGG GAGACTCGTT CTACATCAGG 
ACTCATTTTG AATATGAGAA GGAGTCGCCC TATGGCCTGA GCTTCAATAA GGGGGAGGTC 
TTCAGAGTCG TCGACACCCT TTACAATGGC AAACTGGGCT CCTGGCTCGC CATCCGCATC 
GGCAAAAACC ACCAGGAGGT GGAGCGGGGC ATCATCCCCA ACAAGAACAG GGCTGAGCAG 
TTATCAAGTG TCCAGTACAC TCTCCCAAAG ACTCCAGGAG GGGATCGCGC CGACTTCTGG 
AGATTCAGAG GCCTCCGCAG CTCCAAACGA AACCTGAGGA AGAGCAGAGA AGACCTGTCT 
GCTCAGCCGG TCCAAACCAA ATTCCCTGCT TATGAAAGAG TGGTGCTTAG AGAAGCTGGT 
TTCTTGAGGC CAGTGGTCAT TTTCGGACCG ATCGCGGATG TGGCTAGAGA GAAGCTGTCC 
AGAGAGGAGA CTGACCTCTT CGAACTTGCA AAGAGTGAAC CCAGAGATGC GGGCACAGAC 
CAGCGCAGCT CCGGAATCAT CCGTCTCCAT ACCATTAAAC AGATCATTGA CCGAGACAAG 
CACGCGGTGC TGGACATCAC CCCTAATGCG GTGGACCGTC TGAACTACGC TCAGTGGTAC 
CCCATCGTGG TGTTTCTGAA TCCAGACAGC AAACAGGGCG TCAAGAACAT GAGGACCAGG 
CTCTGTCCCG AGTCCAGGAA GAGCGCCCGA AAGCTCTACG AGCGTGCTCT CAAACTGAGG 
AAGAACAACC ATCACCTTTT CACCACCACT ATTAACTTGA ACAACATGAA TGACGGGTGG 
TACGGTGCTT TGAAAGAGAG CATCCAGCAG CAGCAGAACC AGTTGGTCTG GGTCTCAGAG 
GGCAAGGCCG ACGGCGCTCC TGACGATGAC CTTGACATCC ATGACGACCG CCTCTCCTAC 
CTCTCGGCTC CGGGCAGCGA GTACAGCATG TACAGCACCG ACAGCCGCCA CACGTCCGAC 
TATGAGGACA CCGACACGGA GGGTGGTGCC TACACCGACC AGGAGCTGGA CGAGACCATG 
AACGATGAGG TGGGCCTGCC TAGCGAGCCT GCCATCACAC GCTCATCTGA GCCTGTGCGA 
GAGGACCCGC CAGTCATTCA GGACGCAGCT GGATACCCCA GCTACCAGCA TGCGGTACCG 
CAACCTGAAG CGGTGAGCCG CATTGACCCG GCCGGGTTTA AGATGGCAGC GCCGCAGCAG 
ATGTATAAAA AGGATCTGTA CAGCATGGAC GAGCCGGTGC GAGTGACCCA CGGCGTGAAG 
CCTCCCCCTC CCCAGCAGCA GCAGCAGCCT TTAGGCCCCC CAACCTCGCT CTCCTACAGC 
CACCAGCCTG TCTACCAGGA CCAACAGCCA TACCGCCAGT ACGACCACCC GCCTTATGGC 
TATGACGGTG GCGGCTACGC ACAACCAAAG CCTCACAACT ACGACCCGCA CCTGCACTAC 
GACAACCGTG TGCCTCACTA TAACGAGCAG TGGCCCACGT ACGACCAGCA GCAGCAGCAG 
CAGACTTCCC CCCAGCCGCC ACCTGCGTCC GGCTTCCCTC AGGGCCACCA GCCTCCGCCT 
CCACCACCGA TGGGATATGA GCCACGCTCC CCATACGAGG ACGGACCTAA CAGGGATTTC 
AGCCCTCCTC AGTCCCAGTA TGACGGTGTG TCCCCAATGG GCTACGATCA CCGGCCGCGG 
CACCCCAAAC CTGCACCGGT CCGATATGAA GAGCCTCCAC CTCCACCTCC TCCGGTGTCC 
TACGATGCAC GGTCACCTTT CGAACCCGAG CCGCATGCTT TCCCAGGCAA CACACATCGC 
TCGCCAGACC CACCGAAACA GTATTATGGA GACGCCGCTA TGAGGCCCTT GTACAACCCT 
GGACCTCCGA ACAGAGTGTA CAAACATGAG CCGGTGAACT CTGAGCCTCC TGTACCGCCT 
CCCAAACCTG AATCTGTCCT GTCTCCAGGA GAACCGCCCC ATTCTGCAGC TTCCAAACCA 
TTGCCGCCTC CTCCCAGAGA TGATGACGAT GATGATGACG ATGATGATGA CCCTGCCATG 
AAGCCCCAGT CTGTGCTCAA CAGGGTCAAA ATGTTCGAAA ACAAACGCTC TGTGTCGGTA 
GACCGAGCCA AGGACGCACC TGACGTCACT GGAATTAGGC CCACAGATCT CCCCAAACCT 
GCGAGCACCC CTGGACCTGT GCTCAAAGCC AACTCTCTCA GCAACCTGGA GCAGGACAAA 
CCCTCCTACA GAGCTCCAGA GCCGCAGAAA CCTCAGACGC GTGTTGGGGA CGATGTGGTT 
CGCTCCAACC ACTACGACCC TGATGATGAT GAAGAGTACT ACAGAAAACA GCTCTCATTT 
TTCGACCGCC GCAGTTTCGA CAGTAAGGTC ATGAATCCAC CCAGCGCTGG AGTAAACCGC 
TTCCATGAGC CGCCCAAACC ACCCCAGACA CAGATCGCTT ATCCTTTCGC TAGGACGGAG 
TCTGTGGAGA AGGTGAGTCC TGTGGACAAG AGATACGAGC CTCTGCCGCC AGTCAACCCT 
TCACCTGCTC CATACAGCCA GAACACACCT GTCGCTCCGC CCACATCCCT GCCCAAACTC 
ACCAACATCG AGGTGAACTC TCTGCCAGAT CCTCACAGCT CTCCCAAAGC CAAACCGGAC 
CTGTCTGCTC TGAGAGCTCC GACCCGGGAC GAGCCCATCC AGAGCAGCTA CTTGCCCCCC 
AAGTCCACCA TCAATGGCAT GGATGCTCCT CCCAAAACAC TCGGCGTGCC GACCAGCTAC 
AACCGCTACG TCCCCAAGCC TTACACCAGC TCGGCGCGAC CCTTCGAGCG CAAGTTCGAG 
AGTCCGAAAT TCAACCACAA CCTGCTTCCC AACGACACGC AGTCCAAACC CAGTGTCAAC 
AACAACCTGA AGCCTCAGAT CTCCCCACAA CCCCAGGACA CGGACAGCGG CGTCGACACC 
TTCACTCGCA CCATGGACAA CAGGCCAAAA TATCAGCACA ACAACATCAA CGCCATTCCC 
AAAGCCGTCC CAGTCAGCCC CAGTGCACTG GATGATGATG AAGAAGATGA GGGACACACA 
GTTGTGGCCA CGGCGCGGGG CATCTTTAAC TGTAATGGCG GGGTGTTGAG CTCTATAGAG 
ACGGGCGTCA GCATCATTAT CCCACAGGGA GCCATTCCTG ATAGTGTGGA GCAGGAGATC 
TACTTCAAAG TGTGCCGGGA CAACAGCATC CTGCCTCCTC TGGATAAGGA GAAAGGTGAA 
ACTCTGCTGA GTCCGCTGGT GATGTGTGGT CCTCACGGCC TGAAGTTCCT GAAGCCTGTG 
GAGCTGCGCT TGCCACACTG TGCGTCTATG ACCCCTGATG GTTGGTCTTT TGCTCTAAAA 
TCCTCCGACT CCTCGTCGGG TGACCCCAAA TGCTGGCAAA ACAAGTGTCT ACCAGGAGAC 
CCCAATTACC TGGTGGGCGC AAACTGTGTG TCTGTGCTGA TCGACCACTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp1b ( XM_017354176.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017209665.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X12, mRNA.

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CloneID	ODa57886
Clone ID Related Accession (Same CDS sequence)	XM_017354176.1
Accession Version	XM_017354176.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5637bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tight junction protein ZO-1 isoform X12
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAATT ACCAGAAATA CATTACTGTT ATGCAGCTGG CTCTAGGGGT GACTGCCTAC 
AACAGAGACC GTAGCCTGCC TCCCAGAAAA CATATGTGGG TCTTCCCCAA ACACGACAAA 
GACAAATACG GCCCTGCAGT TTATTATCCG AAGTTCAACG CCTACATCCC CAACCCAGAC 
AAAGTCTCTG TGCTGGATGA ATCTGAGGGA TCGGGCCACG GCAAACCATC CCTGCGCAGA 
ATCAAGGGCC GAATCCACCG CAGCAAGAGC CTGGACAGCA TCGACCTGCT GGACTCCAAC 
AGTGCAGCAA TGGATGAGAC GGTAATATGG GAACAGCACA CTGTAACTCT ACACAGGGCT 
CCAGGGTTTG GCTTCGGGAT CGCCATCTCC GGCGGACGGG ACAACCCTCA CTTCCAGAGC 
GGGGAGACGT CCATCGTCAT ATCAGATGTG CTGAAGGGAG GCCCCGCCGA GGGCCTGCTG 
CAGGAGAATG ACAGAGTGGT GATGGTCAAC GCCGTCTCCA TGGACAATGT GGATCACGCT 
TACGCAGTGC AGCAGCTCCG CAAAAGTGGC AAGAATGCAA AAATAACCAT CAGGAGAAAG 
CGTAAGGTGC AGATCCCGTC AGGCCGGCCG GGTGAACGCG AGACTATGTC AGAGCATGAC 
GAGGACGACG ACAGCTACGA GGATGAGATC TACGAGTCCC GGAGCGCGCG AAGCGGACCC 
AGCGCCTACA GTGGAGGTGG AGGCGGCGGC GGAGGTGGAG GAGCCTCGGG ACGGAGGAGC 
GGCAGAGGCG ACAGGCTGAG TGGGAGGAGG GAGCGGGACA GAGAGCGCAG CGGCTCCAGA 
GAGAGGAGTC TGTCCCCGCG CTCGGACCGC CACTCTGTGT CCTCCAACCT CCCCCCACGG 
CCAGCCAAAG TCACACTCGT CAAATCCCGC AAGAATGAAG CAGAATACGG GCTTCGCCTG 
GCCAGCCACA TCTTCGTGAA GGACATTTCC CCCGAGAGCC TGGCAGCCCG AGACGGAAAC 
ATCCAAGAGG GAGACGTGGT GCTTAAGATT AATGGCACGG TAACAGAGAA TTTGTCCCTG 
ATCGATGCTA AGAAGCTGAT CGAGAGGTCA AAGGGAAAGC TGAAGATGGT GGTGCAGAGA 
GACGAGCGGG CGACGCTGCT GAACATCCCC GATCTGGACG ACAGCATACC TTCTGCTAAT 
GCCTCGGACA GAGATGATAT TTCAGACATC CACTCCGTGG CCTCTGATCA TTCCAACCGA 
TCCCACGACC GGAAGAGGAG CAGCCGCTCT CGTTCTCCAG ACCGCCGCTC CGAACCCTCA 
GACCACTCCA GACACTCTCC ACCACAGATC AGCAACGGCA GGGCCACAGG GCCGACGGCT 
CAGAGAACGC GGCCAGTTCA CAGGAGCCGT GATGAAGACA GGATCTCCAA GGCCGGGCCG 
CTTCCTCTAC CTGCTAAGAT GGTGGAGGAG ATGCCGAAAG AGCAGAGCGC AGCCAGAGAA 
ACAGAGAAAC AGCTCCCCCC TCTGCCAGAG CCCAAACCTG TGTACGCTCA GCCGGGCCAG 
CCGGACGTGG ACCTGCCTGT TAGTCCGGCA GATGCTCCTG TGCCCAGCGT CACACATGAT 
GACAGCATCT TACGGCCGAG CATGAAGCTG GTGAAGTTCA AGAAGGGCGA GAGCGTGGGC 
TTGAGGCTCG CTGGGGGGAA CGACGTTGGG ATATTTGTGG CGGGAGTTCT GGAGGACAGT 
CCTGCTGCTA AAGAGGGCCT GGAAGAGGGA GACCAGATTC TCAGGGTAAA TAATGTGGAT 
TTTGCAAATA TCATCCGAGA GGAGGCAGTG CTGTTCCTGC TGGATCTGCC CAAAGGAGAG 
GAGGTCACCA TCCTGGCCCA GAAGAAGAAA GACGTGTACC GCCGCATTGT AGAGTCTGAC 
GTGGGAGACT CGTTCTACAT CAGGACTCAT TTTGAATATG AGAAGGAGTC GCCCTATGGC 
CTGAGCTTCA ATAAGGGGGA GGTCTTCAGA GTCGTCGACA CCCTTTACAA TGGCAAACTG 
GGCTCCTGGC TCGCCATCCG CATCGGCAAA AACCACCAGG AGGTGGAGCG GGGCATCATC 
CCCAACAAGA ACAGGGCTGA GCAGTTATCA AGTGTCCAGT ACACTCTCCC AAAGACTCCA 
GGAGGGGATC GCGCCGACTT CTGGAGATTC AGAGGCCTCC GCAGCTCCAA ACGAAACCTG 
AGGAAGAGCA GAGAAGACCT GTCTGCTCAG CCGGTCCAAA CCAAATTCCC TGCTTATGAA 
AGAGTGGTGC TTAGAGAAGC TGGTTTCTTG AGGCCAGTGG TCATTTTCGG ACCGATCGCG 
GATGTGGCTA GAGAGAAGCT GTCCAGAGAG GAGACTGACC TCTTCGAACT TGCAAAGAGT 
GAACCCAGAG ATGCGGGCAC AGACCAGCGC AGCTCCGGAA TCATCCGTCT CCATACCATT 
AAACAGATCA TTGACCGAGA CAAGCACGCG GTGCTGGACA TCACCCCTAA TGCGGTGGAC 
CGTCTGAACT ACGCTCAGTG GTACCCCATC GTGGTGTTTC TGAATCCAGA CAGCAAACAG 
GGCGTCAAGA ACATGAGGAC CAGGCTCTGT CCCGAGTCCA GGAAGAGCGC CCGAAAGCTC 
TACGAGCGTG CTCTCAAACT GAGGAAGAAC AACCATCACC TTTTCACCAC CACTATTAAC 
TTGAACAACA TGAATGACGG GTGGTACGGT GCTTTGAAAG AGAGCATCCA GCAGCAGCAG 
AACCAGTTGG TCTGGGTCTC AGAGGGCAAG GCCGACGGCG CTCCTGACGA TGACCTTGAC 
ATCCATGACG ACCGCCTCTC CTACCTCTCG GCTCCGGGCA GCGAGTACAG CATGTACAGC 
ACCGACAGCC GCCACACGTC CGACTATGAG GACACCGACA CGGAGGGTGG TGCCTACACC 
GACCAGGAGC TGGACGAGAC CATGAACGAT GAGGTGGGCC TGCCTAGCGA GCCTGCCATC 
ACACGCTCAT CTGAGCCTGT GCGAGAGGAC CCGCCAGTCA TTCAGGACGC AGCTGGATAC 
CCCAGCTACC AGCATGCGGT ACCGCAACCT GAAGCGGTGA GCCGCATTGA CCCGGCCGGG 
TTTAAGATGG CAGCGCCGCA GCAGCAAGCT GAGGCTGCTC TGGCCTCGCC GCCCCCTCCC 
TCTGCAGCGG TCCAGCCCAC CGCAACACTA GCGGGTACGA ACCCCGAGGA GCCGCCTGGC 
ACCCCTCAGG CCGACTCCCT TAACAGCCCC GTCCCTGTTC CCGACCCCCA GCCCGAACCT 
GAGCTCGCTC AGCCCCCAAC ACACGACCCC CACCAGTCGC TTCCTCCTGG CCCAGATCCA 
AAGATGTATA AAAAGGATCT GTACAGCATG GACGAGCCGG TGCGAGTGAC CCACGGCGTG 
AAGCCTCCCC CTCCCCAGCA GCAGCAGCAG CCTTTAGGCC CCCCAACCTC GCTCTCCTAC 
AGCCACCAGC CTGTCTACCA GGACCAACAG CCATACCGCC AGTACGACCA CCCGCCTTAT 
GGCTATGACG GTGGCGGCTA CGCACAACCA AAGCCTCACA ACTACGACCC GCACCTGCAC 
TACGACAACC GTGTGCCTCA CTATAACGAG CAGTGGCCCA CGTACGACCA GCAGCAGCAG 
CAGCAGACTT CCCCCCAGCC GCCACCTGCG TCCGGCTTCC CTCAGGGCCA CCAGCCTCCG 
CCTCCACCAC CGATGGGATA TGAGCCACGC TCCCCATACG AGGACGGACC TAACAGGGAT 
TTCAGCCCTC CTCAGTCCCA GTATGACGGT GTGTCCCCAA TGGGCTACGA TCACCGGCCG 
CGGCACCCCA AACCTGCACC GGTCCGATAT GAAGAGCCTC CACCTCCACC TCCTCCGGTG 
TCCTACGATG CACGGTCACC TTTCGAACCC GAGCCGCATG CTTTCCCAGG CAACACACAT 
CGCTCGCCAG ACCCACCGAA ACAGTATTAT GGAGACGCCG CTATGAGGCC CTTGTACAAC 
CCTGGACCTC CGAACAGAGT GTACAAACAT GAGCCGGTGA ACTCTGAGCC TCCTGTACCG 
CCTCCCAAAC CTGAATCTGT CCTGTCTCCA GGAGAACCGC CCCATTCTGC AGCTTCCAAA 
CCATTGCCGC CTCCTCCCAG AGATGATGAC GATGATGATG ACGATGATGA TGACCCTGCC 
ATGAAGCCCC AGTCTGTGCT CAACAGGGTC AAAATGTTCG AAAACAAACG CTCTGTGTCG 
GTAGACCGAG CCAAGGACGC ACCTGACGTC ACTGGAATTA GGCCCACAGA TCTCCCCAAA 
CCTGCGAGCA CCCCTGGACC TGTGCTCAAA GCCAACTCTC TCAGCAACCT GGAGCAGGAC 
AAACCCTCCT ACAGAGCTCC AGAGCCGCAG AAACCTCAGA CGCGTGTTGG GGACGATGTG 
GTTCGCTCCA ACCACTACGA CCCTGATGAT GATGAAGAGT ACTACAGAAA ACAGCTCTCA 
TTTTTCGACC GCCGCAGTTT CGACAGTAAG GTCATGAATC CACCCAGCGC TGGAGTAAAC 
CGCTTCCATG AGCCGCCCAA ACCACCCCAG ACACAGATCG CTTATCCTTT CGCTAGGACG 
GAGTCTGTGG AGAAGGTGAG TCCTGTGGAC AAGAGATACG AGCCTCTGCC GCCAGTCAAC 
CCTTCACCTG CTCCATACAG CCAGAACACA CCTGTCGCTC CGCCCACATC CCTGCCCAAA 
CTCACCAACA TCGAGGTGAA CTCTCTGCCA GATCCTCACA GCTCTCCCAA AGCCAAACCG 
GACCTGTCTG CTCTGAGAGC TCCGACCCGG GACGAGCCCA TCCAGAGCAG CTACTTGCCC 
CCCAAGTCCA CCATCAATGG CATGGATGCT CCTCCCAAAA CACTCGGCGT GCCGACCAGC 
TACAACCGCT ACGTCCCCAA GCCTTACACC AGCTCGGCGC GACCCTTCGA GCGCAAGTTC 
GAGAGTCCGA AATTCAACCA CAACCTGCTT CCCAACGACA CGCAGTCCAA ACCCAGTGTC 
AACAACAACC TGAAGCCTCA GATCTCCCCA CAACCCCAGG ACACGGACAG CGGCGTCGAC 
ACCTTCACTC GCACCATGGA CAACAGGCCA AAATATCAGC ACAACAACAT CAACGCCATT 
CCCAAAGCCG TCCCAGTCAG CCCCAGTGCA CTGGATGATG ATGAAGAAGA TGAGGGACAC 
ACAGTTGTGG CCACGGCGCG GGGCATCTTT AACTGTAATG GCGGGGTGTT GAGCTCTATA 
GAGACGGGCG TCAGCATCAT TATCCCACAG GGAGCCATTC CTGATAGTGT GGAGCAGGAG 
ATCTACTTCA AAGTGTGCCG GGACAACAGC ATCCTGCCTC CTCTGGATAA GGAGAAAGGT 
GAAACTCTGC TGAGTCCGCT GGTGATGTGT GGTCCTCACG GCCTGAAGTT CCTGAAGCCT 
GTGGAGCTGC GCTTGCCACA CTGTGCGTCT ATGACCCCTG ATGGTTGGTC TTTTGCTCTA 
AAATCCTCCG ACTCCTCGTC GGGTGACCCC AAATGCTGGC AAAACAAGTG TCTACCAGGA 
GACCCCAATT ACCTGGTGGG CGCAAACTGT GTGTCTGTGC TGATCGACCA CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209665.1
CDS	431..6067
Translation	MVNYQKYITVMQLALGVTAYNRDRSLPPRKHMWVFPKHDKDKYGPAVYYPKFNAYIPNPDKVSVLDESEGSGHGKPSLRRIKGRIHRSKSLDSIDLLDSNSAAMDETVIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGLLQENDRVVMVNAVSMDNVDHAYAVQQLRKSGKNAKITIRRKRKVQIPSGRPGERETMSEHDEDDDSYEDEIYESRSARSGPSAYSGGGGGGGGGGASGRRSGRGDRLSGRRERDRERSGSRERSLSPRSDRHSVSSNLPPRPAKVTLVKSRKNEAEYGLRLASHIFVKDISPESLAARDGNIQEGDVVLKINGTVTENLSLIDAKKLIERSKGKLKMVVQRDERATLLNIPDLDDSIPSANASDRDDISDIHSVASDHSNRSHDRKRSSRSRSPDRRSEPSDHSRHSPPQISNGRATGPTAQRTRPVHRSRDEDRISKAGPLPLPAKMVEEMPKEQSAARETEKQLPPLPEPKPVYAQPGQPDVDLPVSPADAPVPSVTHDDSILRPSMKLVKFKKGESVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFANIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHQEVERGIIPNKNRAEQLSSVQYTLPKTPGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVVIFGPIADVAREKLSREETDLFELAKSEPRDAGTDQRSSGIIRLHTIKQIIDRDKHAVLDITPNAVDRLNYAQWYPIVVFLNPDSKQGVKNMRTRLCPESRKSARKLYERALKLRKNNHHLFTTTINLNNMNDGWYGALKESIQQQQNQLVWVSEGKADGAPDDDLDIHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETMNDEVGLPSEPAITRSSEPVREDPPVIQDAAGYPSYQHAVPQPEAVSRIDPAGFKMAAPQQQAEAALASPPPPSAAVQPTATLAGTNPEEPPGTPQADSLNSPVPVPDPQPEPELAQPPTHDPHQSLPPGPDPKMYKKDLYSMDEPVRVTHGVKPPPPQQQQQPLGPPTSLSYSHQPVYQDQQPYRQYDHPPYGYDGGGYAQPKPHNYDPHLHYDNRVPHYNEQWPTYDQQQQQQTSPQPPPASGFPQGHQPPPPPPMGYEPRSPYEDGPNRDFSPPQSQYDGVSPMGYDHRPRHPKPAPVRYEEPPPPPPPVSYDARSPFEPEPHAFPGNTHRSPDPPKQYYGDAAMRPLYNPGPPNRVYKHEPVNSEPPVPPPKPESVLSPGEPPHSAASKPLPPPPRDDDDDDDDDDDPAMKPQSVLNRVKMFENKRSVSVDRAKDAPDVTGIRPTDLPKPASTPGPVLKANSLSNLEQDKPSYRAPEPQKPQTRVGDDVVRSNHYDPDDDEEYYRKQLSFFDRRSFDSKVMNPPSAGVNRFHEPPKPPQTQIAYPFARTESVEKVSPVDKRYEPLPPVNPSPAPYSQNTPVAPPTSLPKLTNIEVNSLPDPHSSPKAKPDLSALRAPTRDEPIQSSYLPPKSTINGMDAPPKTLGVPTSYNRYVPKPYTSSARPFERKFESPKFNHNLLPNDTQSKPSVNNNLKPQISPQPQDTDSGVDTFTRTMDNRPKYQHNNINAIPKAVPVSPSALDDDEEDEGHTVVATARGIFNCNGGVLSSIETGVSIIIPQGAIPDSVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKCWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_017354176.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tight junction protein 1b (tjp1b), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017354176.1

ORF Insert Sequence:

ATGGTGAATT ACCAGAAATA CATTACTGTT ATGCAGCTGG CTCTAGGGGT GACTGCCTAC 
AACAGAGACC GTAGCCTGCC TCCCAGAAAA CATATGTGGG TCTTCCCCAA ACACGACAAA 
GACAAATACG GCCCTGCAGT TTATTATCCG AAGTTCAACG CCTACATCCC CAACCCAGAC 
AAAGTCTCTG TGCTGGATGA ATCTGAGGGA TCGGGCCACG GCAAACCATC CCTGCGCAGA 
ATCAAGGGCC GAATCCACCG CAGCAAGAGC CTGGACAGCA TCGACCTGCT GGACTCCAAC 
AGTGCAGCAA TGGATGAGAC GGTAATATGG GAACAGCACA CTGTAACTCT ACACAGGGCT 
CCAGGGTTTG GCTTCGGGAT CGCCATCTCC GGCGGACGGG ACAACCCTCA CTTCCAGAGC 
GGGGAGACGT CCATCGTCAT ATCAGATGTG CTGAAGGGAG GCCCCGCCGA GGGCCTGCTG 
CAGGAGAATG ACAGAGTGGT GATGGTCAAC GCCGTCTCCA TGGACAATGT GGATCACGCT 
TACGCAGTGC AGCAGCTCCG CAAAAGTGGC AAGAATGCAA AAATAACCAT CAGGAGAAAG 
CGTAAGGTGC AGATCCCGTC AGGCCGGCCG GGTGAACGCG AGACTATGTC AGAGCATGAC 
GAGGACGACG ACAGCTACGA GGATGAGATC TACGAGTCCC GGAGCGCGCG AAGCGGACCC 
AGCGCCTACA GTGGAGGTGG AGGCGGCGGC GGAGGTGGAG GAGCCTCGGG ACGGAGGAGC 
GGCAGAGGCG ACAGGCTGAG TGGGAGGAGG GAGCGGGACA GAGAGCGCAG CGGCTCCAGA 
GAGAGGAGTC TGTCCCCGCG CTCGGACCGC CACTCTGTGT CCTCCAACCT CCCCCCACGG 
CCAGCCAAAG TCACACTCGT CAAATCCCGC AAGAATGAAG CAGAATACGG GCTTCGCCTG 
GCCAGCCACA TCTTCGTGAA GGACATTTCC CCCGAGAGCC TGGCAGCCCG AGACGGAAAC 
ATCCAAGAGG GAGACGTGGT GCTTAAGATT AATGGCACGG TAACAGAGAA TTTGTCCCTG 
ATCGATGCTA AGAAGCTGAT CGAGAGGTCA AAGGGAAAGC TGAAGATGGT GGTGCAGAGA 
GACGAGCGGG CGACGCTGCT GAACATCCCC GATCTGGACG ACAGCATACC TTCTGCTAAT 
GCCTCGGACA GAGATGATAT TTCAGACATC CACTCCGTGG CCTCTGATCA TTCCAACCGA 
TCCCACGACC GGAAGAGGAG CAGCCGCTCT CGTTCTCCAG ACCGCCGCTC CGAACCCTCA 
GACCACTCCA GACACTCTCC ACCACAGATC AGCAACGGCA GGGCCACAGG GCCGACGGCT 
CAGAGAACGC GGCCAGTTCA CAGGAGCCGT GATGAAGACA GGATCTCCAA GGCCGGGCCG 
CTTCCTCTAC CTGCTAAGAT GGTGGAGGAG ATGCCGAAAG AGCAGAGCGC AGCCAGAGAA 
ACAGAGAAAC AGCTCCCCCC TCTGCCAGAG CCCAAACCTG TGTACGCTCA GCCGGGCCAG 
CCGGACGTGG ACCTGCCTGT TAGTCCGGCA GATGCTCCTG TGCCCAGCGT CACACATGAT 
GACAGCATCT TACGGCCGAG CATGAAGCTG GTGAAGTTCA AGAAGGGCGA GAGCGTGGGC 
TTGAGGCTCG CTGGGGGGAA CGACGTTGGG ATATTTGTGG CGGGAGTTCT GGAGGACAGT 
CCTGCTGCTA AAGAGGGCCT GGAAGAGGGA GACCAGATTC TCAGGGTAAA TAATGTGGAT 
TTTGCAAATA TCATCCGAGA GGAGGCAGTG CTGTTCCTGC TGGATCTGCC CAAAGGAGAG 
GAGGTCACCA TCCTGGCCCA GAAGAAGAAA GACGTGTACC GCCGCATTGT AGAGTCTGAC 
GTGGGAGACT CGTTCTACAT CAGGACTCAT TTTGAATATG AGAAGGAGTC GCCCTATGGC 
CTGAGCTTCA ATAAGGGGGA GGTCTTCAGA GTCGTCGACA CCCTTTACAA TGGCAAACTG 
GGCTCCTGGC TCGCCATCCG CATCGGCAAA AACCACCAGG AGGTGGAGCG GGGCATCATC 
CCCAACAAGA ACAGGGCTGA GCAGTTATCA AGTGTCCAGT ACACTCTCCC AAAGACTCCA 
GGAGGGGATC GCGCCGACTT CTGGAGATTC AGAGGCCTCC GCAGCTCCAA ACGAAACCTG 
AGGAAGAGCA GAGAAGACCT GTCTGCTCAG CCGGTCCAAA CCAAATTCCC TGCTTATGAA 
AGAGTGGTGC TTAGAGAAGC TGGTTTCTTG AGGCCAGTGG TCATTTTCGG ACCGATCGCG 
GATGTGGCTA GAGAGAAGCT GTCCAGAGAG GAGACTGACC TCTTCGAACT TGCAAAGAGT 
GAACCCAGAG ATGCGGGCAC AGACCAGCGC AGCTCCGGAA TCATCCGTCT CCATACCATT 
AAACAGATCA TTGACCGAGA CAAGCACGCG GTGCTGGACA TCACCCCTAA TGCGGTGGAC 
CGTCTGAACT ACGCTCAGTG GTACCCCATC GTGGTGTTTC TGAATCCAGA CAGCAAACAG 
GGCGTCAAGA ACATGAGGAC CAGGCTCTGT CCCGAGTCCA GGAAGAGCGC CCGAAAGCTC 
TACGAGCGTG CTCTCAAACT GAGGAAGAAC AACCATCACC TTTTCACCAC CACTATTAAC 
TTGAACAACA TGAATGACGG GTGGTACGGT GCTTTGAAAG AGAGCATCCA GCAGCAGCAG 
AACCAGTTGG TCTGGGTCTC AGAGGGCAAG GCCGACGGCG CTCCTGACGA TGACCTTGAC 
ATCCATGACG ACCGCCTCTC CTACCTCTCG GCTCCGGGCA GCGAGTACAG CATGTACAGC 
ACCGACAGCC GCCACACGTC CGACTATGAG GACACCGACA CGGAGGGTGG TGCCTACACC 
GACCAGGAGC TGGACGAGAC CATGAACGAT GAGGTGGGCC TGCCTAGCGA GCCTGCCATC 
ACACGCTCAT CTGAGCCTGT GCGAGAGGAC CCGCCAGTCA TTCAGGACGC AGCTGGATAC 
CCCAGCTACC AGCATGCGGT ACCGCAACCT GAAGCGGTGA GCCGCATTGA CCCGGCCGGG 
TTTAAGATGG CAGCGCCGCA GCAGCAAGCT GAGGCTGCTC TGGCCTCGCC GCCCCCTCCC 
TCTGCAGCGG TCCAGCCCAC CGCAACACTA GCGGGTACGA ACCCCGAGGA GCCGCCTGGC 
ACCCCTCAGG CCGACTCCCT TAACAGCCCC GTCCCTGTTC CCGACCCCCA GCCCGAACCT 
GAGCTCGCTC AGCCCCCAAC ACACGACCCC CACCAGTCGC TTCCTCCTGG CCCAGATCCA 
AAGATGTATA AAAAGGATCT GTACAGCATG GACGAGCCGG TGCGAGTGAC CCACGGCGTG 
AAGCCTCCCC CTCCCCAGCA GCAGCAGCAG CCTTTAGGCC CCCCAACCTC GCTCTCCTAC 
AGCCACCAGC CTGTCTACCA GGACCAACAG CCATACCGCC AGTACGACCA CCCGCCTTAT 
GGCTATGACG GTGGCGGCTA CGCACAACCA AAGCCTCACA ACTACGACCC GCACCTGCAC 
TACGACAACC GTGTGCCTCA CTATAACGAG CAGTGGCCCA CGTACGACCA GCAGCAGCAG 
CAGCAGACTT CCCCCCAGCC GCCACCTGCG TCCGGCTTCC CTCAGGGCCA CCAGCCTCCG 
CCTCCACCAC CGATGGGATA TGAGCCACGC TCCCCATACG AGGACGGACC TAACAGGGAT 
TTCAGCCCTC CTCAGTCCCA GTATGACGGT GTGTCCCCAA TGGGCTACGA TCACCGGCCG 
CGGCACCCCA AACCTGCACC GGTCCGATAT GAAGAGCCTC CACCTCCACC TCCTCCGGTG 
TCCTACGATG CACGGTCACC TTTCGAACCC GAGCCGCATG CTTTCCCAGG CAACACACAT 
CGCTCGCCAG ACCCACCGAA ACAGTATTAT GGAGACGCCG CTATGAGGCC CTTGTACAAC 
CCTGGACCTC CGAACAGAGT GTACAAACAT GAGCCGGTGA ACTCTGAGCC TCCTGTACCG 
CCTCCCAAAC CTGAATCTGT CCTGTCTCCA GGAGAACCGC CCCATTCTGC AGCTTCCAAA 
CCATTGCCGC CTCCTCCCAG AGATGATGAC GATGATGATG ACGATGATGA TGACCCTGCC 
ATGAAGCCCC AGTCTGTGCT CAACAGGGTC AAAATGTTCG AAAACAAACG CTCTGTGTCG 
GTAGACCGAG CCAAGGACGC ACCTGACGTC ACTGGAATTA GGCCCACAGA TCTCCCCAAA 
CCTGCGAGCA CCCCTGGACC TGTGCTCAAA GCCAACTCTC TCAGCAACCT GGAGCAGGAC 
AAACCCTCCT ACAGAGCTCC AGAGCCGCAG AAACCTCAGA CGCGTGTTGG GGACGATGTG 
GTTCGCTCCA ACCACTACGA CCCTGATGAT GATGAAGAGT ACTACAGAAA ACAGCTCTCA 
TTTTTCGACC GCCGCAGTTT CGACAGTAAG GTCATGAATC CACCCAGCGC TGGAGTAAAC 
CGCTTCCATG AGCCGCCCAA ACCACCCCAG ACACAGATCG CTTATCCTTT CGCTAGGACG 
GAGTCTGTGG AGAAGGTGAG TCCTGTGGAC AAGAGATACG AGCCTCTGCC GCCAGTCAAC 
CCTTCACCTG CTCCATACAG CCAGAACACA CCTGTCGCTC CGCCCACATC CCTGCCCAAA 
CTCACCAACA TCGAGGTGAA CTCTCTGCCA GATCCTCACA GCTCTCCCAA AGCCAAACCG 
GACCTGTCTG CTCTGAGAGC TCCGACCCGG GACGAGCCCA TCCAGAGCAG CTACTTGCCC 
CCCAAGTCCA CCATCAATGG CATGGATGCT CCTCCCAAAA CACTCGGCGT GCCGACCAGC 
TACAACCGCT ACGTCCCCAA GCCTTACACC AGCTCGGCGC GACCCTTCGA GCGCAAGTTC 
GAGAGTCCGA AATTCAACCA CAACCTGCTT CCCAACGACA CGCAGTCCAA ACCCAGTGTC 
AACAACAACC TGAAGCCTCA GATCTCCCCA CAACCCCAGG ACACGGACAG CGGCGTCGAC 
ACCTTCACTC GCACCATGGA CAACAGGCCA AAATATCAGC ACAACAACAT CAACGCCATT 
CCCAAAGCCG TCCCAGTCAG CCCCAGTGCA CTGGATGATG ATGAAGAAGA TGAGGGACAC 
ACAGTTGTGG CCACGGCGCG GGGCATCTTT AACTGTAATG GCGGGGTGTT GAGCTCTATA 
GAGACGGGCG TCAGCATCAT TATCCCACAG GGAGCCATTC CTGATAGTGT GGAGCAGGAG 
ATCTACTTCA AAGTGTGCCG GGACAACAGC ATCCTGCCTC CTCTGGATAA GGAGAAAGGT 
GAAACTCTGC TGAGTCCGCT GGTGATGTGT GGTCCTCACG GCCTGAAGTT CCTGAAGCCT 
GTGGAGCTGC GCTTGCCACA CTGTGCGTCT ATGACCCCTG ATGGTTGGTC TTTTGCTCTA 
AAATCCTCCG ACTCCTCGTC GGGTGACCCC AAATGCTGGC AAAACAAGTG TCTACCAGGA 
GACCCCAATT ACCTGGTGGG CGCAAACTGT GTGTCTGTGC TGATCGACCA CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp1b ( XM_017354177.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017209666.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X13, mRNA.

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CloneID	ODa57887
Clone ID Related Accession (Same CDS sequence)	XM_017354177.1
Accession Version	XM_017354177.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5364bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tight junction protein ZO-1 isoform X13
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCCC GGGCTGCCTC TAATAAGAGT GCAGCAATGG ATGAGACGGT AATATGGGAA 
CAGCACACTG TAACTCTACA CAGGGCTCCA GGGTTTGGCT TCGGGATCGC CATCTCCGGC 
GGACGGGACA ACCCTCACTT CCAGAGCGGG GAGACGTCCA TCGTCATATC AGATGTGCTG 
AAGGGAGGCC CCGCCGAGGG CCTGCTGCAG GAGAATGACA GAGTGGTGAT GGTCAACGCC 
GTCTCCATGG ACAATGTGGA TCACGCTTAC GCAGTGCAGC AGCTCCGCAA AAGTGGCAAG 
AATGCAAAAA TAACCATCAG GAGAAAGCGT AAGGTGCAGA TCCCGTCAGG CCGGCCGGGT 
GAACGCGAGA CTATGTCAGA GCATGACGAG GACGACGACA GCTACGAGGA TGAGATCTAC 
GAGTCCCGGA GCGCGCGAAG CGGACCCAGC GCCTACAGTG GAGGTGGAGG CGGCGGCGGA 
GGTGGAGGAG CCTCGGGACG GAGGAGCGGC AGAGGCGACA GGCTGAGTGG GAGGAGGGAG 
CGGGACAGAG AGCGCAGCGG CTCCAGAGAG AGGAGTCTGT CCCCGCGCTC GGACCGCCAC 
TCTGTGTCCT CCAACCTCCC CCCACGGCCA GCCAAAGTCA CACTCGTCAA ATCCCGCAAG 
AATGAAGCAG AATACGGGCT TCGCCTGGCC AGCCACATCT TCGTGAAGGA CATTTCCCCC 
GAGAGCCTGG CAGCCCGAGA CGGAAACATC CAAGAGGGAG ACGTGGTGCT TAAGATTAAT 
GGCACGGTAA CAGAGAATTT GTCCCTGATC GATGCTAAGA AGCTGATCGA GAGGTCAAAG 
GGAAAGCTGA AGATGGTGGT GCAGAGAGAC GAGCGGGCGA CGCTGCTGAA CATCCCCGAT 
CTGGACGACA GCATACCTTC TGCTAATGCC TCGGACAGAG ATGATATTTC AGACATCCAC 
TCCGTGGCCT CTGATCATTC CAACCGATCC CACGACCGGA AGAGGAGCAG CCGCTCTCGT 
TCTCCAGACC GCCGCTCCGA ACCCTCAGAC CACTCCAGAC ACTCTCCACC ACAGATCAGC 
AACGGCAGGG CCACAGGGCC GACGGCTCAG AGAACGCGGC CAGTTCACAG GAGCCGTGAT 
GAAGACAGGA TCTCCAAGGC CGGGCCGCTT CCTCTACCTG CTAAGATGGT GGAGGAGATG 
CCGAAAGAGC AGAGCGCAGC CAGAGAAACA GAGAAACAGC TCCCCCCTCT GCCAGAGCCC 
AAACCTGTGT ACGCTCAGCC GGGCCAGCCG GACGTGGACC TGCCTGTTAG TCCGGCAGAT 
GCTCCTGTGC CCAGCGTCAC ACATGATGAC AGCATCTTAC GGCCGAGCAT GAAGCTGGTG 
AAGTTCAAGA AGGGCGAGAG CGTGGGCTTG AGGCTCGCTG GGGGGAACGA CGTTGGGATA 
TTTGTGGCGG GAGTTCTGGA GGACAGTCCT GCTGCTAAAG AGGGCCTGGA AGAGGGAGAC 
CAGATTCTCA GGGTAAATAA TGTGGATTTT GCAAATATCA TCCGAGAGGA GGCAGTGCTG 
TTCCTGCTGG ATCTGCCCAA AGGAGAGGAG GTCACCATCC TGGCCCAGAA GAAGAAAGAC 
GTGTACCGCC GCATTGTAGA GTCTGACGTG GGAGACTCGT TCTACATCAG GACTCATTTT 
GAATATGAGA AGGAGTCGCC CTATGGCCTG AGCTTCAATA AGGGGGAGGT CTTCAGAGTC 
GTCGACACCC TTTACAATGG CAAACTGGGC TCCTGGCTCG CCATCCGCAT CGGCAAAAAC 
CACCAGGAGG TGGAGCGGGG CATCATCCCC AACAAGAACA GGGCTGAGCA GTTATCAAGT 
GTCCAGTACA CTCTCCCAAA GACTCCAGGA GGGGATCGCG CCGACTTCTG GAGATTCAGA 
GGCCTCCGCA GCTCCAAACG AAACCTGAGG AAGAGCAGAG AAGACCTGTC TGCTCAGCCG 
GTCCAAACCA AATTCCCTGC TTATGAAAGA GTGGTGCTTA GAGAAGCTGG TTTCTTGAGG 
CCAGTGGTCA TTTTCGGACC GATCGCGGAT GTGGCTAGAG AGAAGCTGTC CAGAGAGGAG 
ACTGACCTCT TCGAACTTGC AAAGAGTGAA CCCAGAGATG CGGGCACAGA CCAGCGCAGC 
TCCGGAATCA TCCGTCTCCA TACCATTAAA CAGATCATTG ACCGAGACAA GCACGCGGTG 
CTGGACATCA CCCCTAATGC GGTGGACCGT CTGAACTACG CTCAGTGGTA CCCCATCGTG 
GTGTTTCTGA ATCCAGACAG CAAACAGGGC GTCAAGAACA TGAGGACCAG GCTCTGTCCC 
GAGTCCAGGA AGAGCGCCCG AAAGCTCTAC GAGCGTGCTC TCAAACTGAG GAAGAACAAC 
CATCACCTTT TCACCACCAC TATTAACTTG AACAACATGA ATGACGGGTG GTACGGTGCT 
TTGAAAGAGA GCATCCAGCA GCAGCAGAAC CAGTTGGTCT GGGTCTCAGA GGGCAAGGCC 
GACGGCGCTC CTGACGATGA CCTTGACATC CATGACGACC GCCTCTCCTA CCTCTCGGCT 
CCGGGCAGCG AGTACAGCAT GTACAGCACC GACAGCCGCC ACACGTCCGA CTATGAGGAC 
ACCGACACGG AGGGTGGTGC CTACACCGAC CAGGAGCTGG ACGAGACCAT GAACGATGAG 
GTGGGCCTGC CTAGCGAGCC TGCCATCACA CGCTCATCTG AGCCTGTGCG AGAGGACCCG 
CCAGTCATTC AGGACGCAGC TGGATACCCC AGCTACCAGC ATGCGGTACC GCAACCTGAA 
GCGGTGAGCC GCATTGACCC GGCCGGGTTT AAGATGGCAG CGCCGCAGCA GCAAGCTGAG 
GCTGCTCTGG CCTCGCCGCC CCCTCCCTCT GCAGCGGTCC AGCCCACCGC AACACTAGCG 
GGTACGAACC CCGAGGAGCC GCCTGGCACC CCTCAGGCCG ACTCCCTTAA CAGCCCCGTC 
CCTGTTCCCG ACCCCCAGCC CGAACCTGAG CTCGCTCAGC CCCCAACACA CGACCCCCAC 
CAGTCGCTTC CTCCTGGCCC AGATCCAAAG ATGTATAAAA AGGATCTGTA CAGCATGGAC 
GAGCCGGTGC GAGTGACCCA CGGCGTGAAG CCTCCCCCTC CCCAGCAGCA GCAGCAGCCT 
TTAGGCCCCC CAACCTCGCT CTCCTACAGC CACCAGCCTG TCTACCAGGA CCAACAGCCA 
TACCGCCAGT ACGACCACCC GCCTTATGGC TATGACGGTG GCGGCTACGC ACAACCAAAG 
CCTCACAACT ACGACCCGCA CCTGCACTAC GACAACCGTG TGCCTCACTA TAACGAGCAG 
TGGCCCACGT ACGACCAGCA GCAGCAGCAG CAGACTTCCC CCCAGCCGCC ACCTGCGTCC 
GGCTTCCCTC AGGGCCACCA GCCTCCGCCT CCACCACCGA TGGGATATGA GCCACGCTCC 
CCATACGAGG ACGGACCTAA CAGGGATTTC AGCCCTCCTC AGTCCCAGTA TGACGGTGTG 
TCCCCAATGG GCTACGATCA CCGGCCGCGG CACCCCAAAC CTGCACCGGT CCGATATGAA 
GAGCCTCCAC CTCCACCTCC TCCGGTGTCC TACGATGCAC GGTCACCTTT CGAACCCGAG 
CCGCATGCTT TCCCAGGCAA CACACATCGC TCGCCAGACC CACCGAAACA GTATTATGGA 
GACGCCGCTA TGAGGCCCTT GTACAACCCT GGACCTCCGA ACAGAGTGTA CAAACATGAG 
CCGGTGAACT CTGAGCCTCC TGTACCGCCT CCCAAACCTG AATCTGTCCT GTCTCCAGGA 
GAACCGCCCC ATTCTGCAGC TTCCAAACCA TTGCCGCCTC CTCCCAGAGA TGATGACGAT 
GATGATGACG ATGATGATGA CCCTGCCATG AAGCCCCAGT CTGTGCTCAA CAGGGTCAAA 
ATGTTCGAAA ACAAACGCTC TGTGTCGGTA GACCGAGCCA AGGACGCACC TGACGTCACT 
GGAATTAGGC CCACAGATCT CCCCAAACCT GCGAGCACCC CTGGACCTGT GCTCAAAGCC 
AACTCTCTCA GCAACCTGGA GCAGGACAAA CCCTCCTACA GAGCTCCAGA GCCGCAGAAA 
CCTCAGACGC GTGTTGGGGA CGATGTGGTT CGCTCCAACC ACTACGACCC TGATGATGAT 
GAAGAGTACT ACAGAAAACA GCTCTCATTT TTCGACCGCC GCAGTTTCGA CAGTAAGGTC 
ATGAATCCAC CCAGCGCTGG AGTAAACCGC TTCCATGAGC CGCCCAAACC ACCCCAGACA 
CAGATCGCTT ATCCTTTCGC TAGGACGGAG TCTGTGGAGA AGGTGAGTCC TGTGGACAAG 
AGATACGAGC CTCTGCCGCC AGTCAACCCT TCACCTGCTC CATACAGCCA GAACACACCT 
GTCGCTCCGC CCACATCCCT GCCCAAACTC ACCAACATCG AGGTGAACTC TCTGCCAGAT 
CCTCACAGCT CTCCCAAAGC CAAACCGGAC CTGTCTGCTC TGAGAGCTCC GACCCGGGAC 
GAGCCCATCC AGAGCAGCTA CTTGCCCCCC AAGTCCACCA TCAATGGCAT GGATGCTCCT 
CCCAAAACAC TCGGCGTGCC GACCAGCTAC AACCGCTACG TCCCCAAGCC TTACACCAGC 
TCGGCGCGAC CCTTCGAGCG CAAGTTCGAG AGTCCGAAAT TCAACCACAA CCTGCTTCCC 
AACGACACGC AGTCCAAACC CAGTGTCAAC AACAACCTGA AGCCTCAGAT CTCCCCACAA 
CCCCAGGACA CGGACAGCGG CGTCGACACC TTCACTCGCA CCATGGACAA CAGGCCAAAA 
TATCAGCACA ACAACATCAA CGCCATTCCC AAAGCCGTCC CAGTCAGCCC CAGTGCACTG 
GATGATGATG AAGAAGATGA GGGACACACA GTTGTGGCCA CGGCGCGGGG CATCTTTAAC 
TGTAATGGCG GGGTGTTGAG CTCTATAGAG ACGGGCGTCA GCATCATTAT CCCACAGGGA 
GCCATTCCTG ATAGTGTGGA GCAGGAGATC TACTTCAAAG TGTGCCGGGA CAACAGCATC 
CTGCCTCCTC TGGATAAGGA GAAAGGTGAA ACTCTGCTGA GTCCGCTGGT GATGTGTGGT 
CCTCACGGCC TGAAGTTCCT GAAGCCTGTG GAGCTGCGCT TGCCACACTG TGCGTCTATG 
ACCCCTGATG GTTGGTCTTT TGCTCTAAAA TCCTCCGACT CCTCGTCGGG TGACCCCAAA 
TGCTGGCAAA ACAAGTGTCT ACCAGGAGAC CCCAATTACC TGGTGGGCGC AAACTGTGTG 
TCTGTGCTGA TCGACCACTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209666.1
CDS	524..5887
Translation	MSARAASNKSAAMDETVIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGLLQENDRVVMVNAVSMDNVDHAYAVQQLRKSGKNAKITIRRKRKVQIPSGRPGERETMSEHDEDDDSYEDEIYESRSARSGPSAYSGGGGGGGGGGASGRRSGRGDRLSGRRERDRERSGSRERSLSPRSDRHSVSSNLPPRPAKVTLVKSRKNEAEYGLRLASHIFVKDISPESLAARDGNIQEGDVVLKINGTVTENLSLIDAKKLIERSKGKLKMVVQRDERATLLNIPDLDDSIPSANASDRDDISDIHSVASDHSNRSHDRKRSSRSRSPDRRSEPSDHSRHSPPQISNGRATGPTAQRTRPVHRSRDEDRISKAGPLPLPAKMVEEMPKEQSAARETEKQLPPLPEPKPVYAQPGQPDVDLPVSPADAPVPSVTHDDSILRPSMKLVKFKKGESVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFANIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHQEVERGIIPNKNRAEQLSSVQYTLPKTPGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVVIFGPIADVAREKLSREETDLFELAKSEPRDAGTDQRSSGIIRLHTIKQIIDRDKHAVLDITPNAVDRLNYAQWYPIVVFLNPDSKQGVKNMRTRLCPESRKSARKLYERALKLRKNNHHLFTTTINLNNMNDGWYGALKESIQQQQNQLVWVSEGKADGAPDDDLDIHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETMNDEVGLPSEPAITRSSEPVREDPPVIQDAAGYPSYQHAVPQPEAVSRIDPAGFKMAAPQQQAEAALASPPPPSAAVQPTATLAGTNPEEPPGTPQADSLNSPVPVPDPQPEPELAQPPTHDPHQSLPPGPDPKMYKKDLYSMDEPVRVTHGVKPPPPQQQQQPLGPPTSLSYSHQPVYQDQQPYRQYDHPPYGYDGGGYAQPKPHNYDPHLHYDNRVPHYNEQWPTYDQQQQQQTSPQPPPASGFPQGHQPPPPPPMGYEPRSPYEDGPNRDFSPPQSQYDGVSPMGYDHRPRHPKPAPVRYEEPPPPPPPVSYDARSPFEPEPHAFPGNTHRSPDPPKQYYGDAAMRPLYNPGPPNRVYKHEPVNSEPPVPPPKPESVLSPGEPPHSAASKPLPPPPRDDDDDDDDDDDPAMKPQSVLNRVKMFENKRSVSVDRAKDAPDVTGIRPTDLPKPASTPGPVLKANSLSNLEQDKPSYRAPEPQKPQTRVGDDVVRSNHYDPDDDEEYYRKQLSFFDRRSFDSKVMNPPSAGVNRFHEPPKPPQTQIAYPFARTESVEKVSPVDKRYEPLPPVNPSPAPYSQNTPVAPPTSLPKLTNIEVNSLPDPHSSPKAKPDLSALRAPTRDEPIQSSYLPPKSTINGMDAPPKTLGVPTSYNRYVPKPYTSSARPFERKFESPKFNHNLLPNDTQSKPSVNNNLKPQISPQPQDTDSGVDTFTRTMDNRPKYQHNNINAIPKAVPVSPSALDDDEEDEGHTVVATARGIFNCNGGVLSSIETGVSIIIPQGAIPDSVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKCWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_017354177.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tight junction protein 1b (tjp1b), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017354177.1

ORF Insert Sequence:

ATGTCTGCCC GGGCTGCCTC TAATAAGAGT GCAGCAATGG ATGAGACGGT AATATGGGAA 
CAGCACACTG TAACTCTACA CAGGGCTCCA GGGTTTGGCT TCGGGATCGC CATCTCCGGC 
GGACGGGACA ACCCTCACTT CCAGAGCGGG GAGACGTCCA TCGTCATATC AGATGTGCTG 
AAGGGAGGCC CCGCCGAGGG CCTGCTGCAG GAGAATGACA GAGTGGTGAT GGTCAACGCC 
GTCTCCATGG ACAATGTGGA TCACGCTTAC GCAGTGCAGC AGCTCCGCAA AAGTGGCAAG 
AATGCAAAAA TAACCATCAG GAGAAAGCGT AAGGTGCAGA TCCCGTCAGG CCGGCCGGGT 
GAACGCGAGA CTATGTCAGA GCATGACGAG GACGACGACA GCTACGAGGA TGAGATCTAC 
GAGTCCCGGA GCGCGCGAAG CGGACCCAGC GCCTACAGTG GAGGTGGAGG CGGCGGCGGA 
GGTGGAGGAG CCTCGGGACG GAGGAGCGGC AGAGGCGACA GGCTGAGTGG GAGGAGGGAG 
CGGGACAGAG AGCGCAGCGG CTCCAGAGAG AGGAGTCTGT CCCCGCGCTC GGACCGCCAC 
TCTGTGTCCT CCAACCTCCC CCCACGGCCA GCCAAAGTCA CACTCGTCAA ATCCCGCAAG 
AATGAAGCAG AATACGGGCT TCGCCTGGCC AGCCACATCT TCGTGAAGGA CATTTCCCCC 
GAGAGCCTGG CAGCCCGAGA CGGAAACATC CAAGAGGGAG ACGTGGTGCT TAAGATTAAT 
GGCACGGTAA CAGAGAATTT GTCCCTGATC GATGCTAAGA AGCTGATCGA GAGGTCAAAG 
GGAAAGCTGA AGATGGTGGT GCAGAGAGAC GAGCGGGCGA CGCTGCTGAA CATCCCCGAT 
CTGGACGACA GCATACCTTC TGCTAATGCC TCGGACAGAG ATGATATTTC AGACATCCAC 
TCCGTGGCCT CTGATCATTC CAACCGATCC CACGACCGGA AGAGGAGCAG CCGCTCTCGT 
TCTCCAGACC GCCGCTCCGA ACCCTCAGAC CACTCCAGAC ACTCTCCACC ACAGATCAGC 
AACGGCAGGG CCACAGGGCC GACGGCTCAG AGAACGCGGC CAGTTCACAG GAGCCGTGAT 
GAAGACAGGA TCTCCAAGGC CGGGCCGCTT CCTCTACCTG CTAAGATGGT GGAGGAGATG 
CCGAAAGAGC AGAGCGCAGC CAGAGAAACA GAGAAACAGC TCCCCCCTCT GCCAGAGCCC 
AAACCTGTGT ACGCTCAGCC GGGCCAGCCG GACGTGGACC TGCCTGTTAG TCCGGCAGAT 
GCTCCTGTGC CCAGCGTCAC ACATGATGAC AGCATCTTAC GGCCGAGCAT GAAGCTGGTG 
AAGTTCAAGA AGGGCGAGAG CGTGGGCTTG AGGCTCGCTG GGGGGAACGA CGTTGGGATA 
TTTGTGGCGG GAGTTCTGGA GGACAGTCCT GCTGCTAAAG AGGGCCTGGA AGAGGGAGAC 
CAGATTCTCA GGGTAAATAA TGTGGATTTT GCAAATATCA TCCGAGAGGA GGCAGTGCTG 
TTCCTGCTGG ATCTGCCCAA AGGAGAGGAG GTCACCATCC TGGCCCAGAA GAAGAAAGAC 
GTGTACCGCC GCATTGTAGA GTCTGACGTG GGAGACTCGT TCTACATCAG GACTCATTTT 
GAATATGAGA AGGAGTCGCC CTATGGCCTG AGCTTCAATA AGGGGGAGGT CTTCAGAGTC 
GTCGACACCC TTTACAATGG CAAACTGGGC TCCTGGCTCG CCATCCGCAT CGGCAAAAAC 
CACCAGGAGG TGGAGCGGGG CATCATCCCC AACAAGAACA GGGCTGAGCA GTTATCAAGT 
GTCCAGTACA CTCTCCCAAA GACTCCAGGA GGGGATCGCG CCGACTTCTG GAGATTCAGA 
GGCCTCCGCA GCTCCAAACG AAACCTGAGG AAGAGCAGAG AAGACCTGTC TGCTCAGCCG 
GTCCAAACCA AATTCCCTGC TTATGAAAGA GTGGTGCTTA GAGAAGCTGG TTTCTTGAGG 
CCAGTGGTCA TTTTCGGACC GATCGCGGAT GTGGCTAGAG AGAAGCTGTC CAGAGAGGAG 
ACTGACCTCT TCGAACTTGC AAAGAGTGAA CCCAGAGATG CGGGCACAGA CCAGCGCAGC 
TCCGGAATCA TCCGTCTCCA TACCATTAAA CAGATCATTG ACCGAGACAA GCACGCGGTG 
CTGGACATCA CCCCTAATGC GGTGGACCGT CTGAACTACG CTCAGTGGTA CCCCATCGTG 
GTGTTTCTGA ATCCAGACAG CAAACAGGGC GTCAAGAACA TGAGGACCAG GCTCTGTCCC 
GAGTCCAGGA AGAGCGCCCG AAAGCTCTAC GAGCGTGCTC TCAAACTGAG GAAGAACAAC 
CATCACCTTT TCACCACCAC TATTAACTTG AACAACATGA ATGACGGGTG GTACGGTGCT 
TTGAAAGAGA GCATCCAGCA GCAGCAGAAC CAGTTGGTCT GGGTCTCAGA GGGCAAGGCC 
GACGGCGCTC CTGACGATGA CCTTGACATC CATGACGACC GCCTCTCCTA CCTCTCGGCT 
CCGGGCAGCG AGTACAGCAT GTACAGCACC GACAGCCGCC ACACGTCCGA CTATGAGGAC 
ACCGACACGG AGGGTGGTGC CTACACCGAC CAGGAGCTGG ACGAGACCAT GAACGATGAG 
GTGGGCCTGC CTAGCGAGCC TGCCATCACA CGCTCATCTG AGCCTGTGCG AGAGGACCCG 
CCAGTCATTC AGGACGCAGC TGGATACCCC AGCTACCAGC ATGCGGTACC GCAACCTGAA 
GCGGTGAGCC GCATTGACCC GGCCGGGTTT AAGATGGCAG CGCCGCAGCA GCAAGCTGAG 
GCTGCTCTGG CCTCGCCGCC CCCTCCCTCT GCAGCGGTCC AGCCCACCGC AACACTAGCG 
GGTACGAACC CCGAGGAGCC GCCTGGCACC CCTCAGGCCG ACTCCCTTAA CAGCCCCGTC 
CCTGTTCCCG ACCCCCAGCC CGAACCTGAG CTCGCTCAGC CCCCAACACA CGACCCCCAC 
CAGTCGCTTC CTCCTGGCCC AGATCCAAAG ATGTATAAAA AGGATCTGTA CAGCATGGAC 
GAGCCGGTGC GAGTGACCCA CGGCGTGAAG CCTCCCCCTC CCCAGCAGCA GCAGCAGCCT 
TTAGGCCCCC CAACCTCGCT CTCCTACAGC CACCAGCCTG TCTACCAGGA CCAACAGCCA 
TACCGCCAGT ACGACCACCC GCCTTATGGC TATGACGGTG GCGGCTACGC ACAACCAAAG 
CCTCACAACT ACGACCCGCA CCTGCACTAC GACAACCGTG TGCCTCACTA TAACGAGCAG 
TGGCCCACGT ACGACCAGCA GCAGCAGCAG CAGACTTCCC CCCAGCCGCC ACCTGCGTCC 
GGCTTCCCTC AGGGCCACCA GCCTCCGCCT CCACCACCGA TGGGATATGA GCCACGCTCC 
CCATACGAGG ACGGACCTAA CAGGGATTTC AGCCCTCCTC AGTCCCAGTA TGACGGTGTG 
TCCCCAATGG GCTACGATCA CCGGCCGCGG CACCCCAAAC CTGCACCGGT CCGATATGAA 
GAGCCTCCAC CTCCACCTCC TCCGGTGTCC TACGATGCAC GGTCACCTTT CGAACCCGAG 
CCGCATGCTT TCCCAGGCAA CACACATCGC TCGCCAGACC CACCGAAACA GTATTATGGA 
GACGCCGCTA TGAGGCCCTT GTACAACCCT GGACCTCCGA ACAGAGTGTA CAAACATGAG 
CCGGTGAACT CTGAGCCTCC TGTACCGCCT CCCAAACCTG AATCTGTCCT GTCTCCAGGA 
GAACCGCCCC ATTCTGCAGC TTCCAAACCA TTGCCGCCTC CTCCCAGAGA TGATGACGAT 
GATGATGACG ATGATGATGA CCCTGCCATG AAGCCCCAGT CTGTGCTCAA CAGGGTCAAA 
ATGTTCGAAA ACAAACGCTC TGTGTCGGTA GACCGAGCCA AGGACGCACC TGACGTCACT 
GGAATTAGGC CCACAGATCT CCCCAAACCT GCGAGCACCC CTGGACCTGT GCTCAAAGCC 
AACTCTCTCA GCAACCTGGA GCAGGACAAA CCCTCCTACA GAGCTCCAGA GCCGCAGAAA 
CCTCAGACGC GTGTTGGGGA CGATGTGGTT CGCTCCAACC ACTACGACCC TGATGATGAT 
GAAGAGTACT ACAGAAAACA GCTCTCATTT TTCGACCGCC GCAGTTTCGA CAGTAAGGTC 
ATGAATCCAC CCAGCGCTGG AGTAAACCGC TTCCATGAGC CGCCCAAACC ACCCCAGACA 
CAGATCGCTT ATCCTTTCGC TAGGACGGAG TCTGTGGAGA AGGTGAGTCC TGTGGACAAG 
AGATACGAGC CTCTGCCGCC AGTCAACCCT TCACCTGCTC CATACAGCCA GAACACACCT 
GTCGCTCCGC CCACATCCCT GCCCAAACTC ACCAACATCG AGGTGAACTC TCTGCCAGAT 
CCTCACAGCT CTCCCAAAGC CAAACCGGAC CTGTCTGCTC TGAGAGCTCC GACCCGGGAC 
GAGCCCATCC AGAGCAGCTA CTTGCCCCCC AAGTCCACCA TCAATGGCAT GGATGCTCCT 
CCCAAAACAC TCGGCGTGCC GACCAGCTAC AACCGCTACG TCCCCAAGCC TTACACCAGC 
TCGGCGCGAC CCTTCGAGCG CAAGTTCGAG AGTCCGAAAT TCAACCACAA CCTGCTTCCC 
AACGACACGC AGTCCAAACC CAGTGTCAAC AACAACCTGA AGCCTCAGAT CTCCCCACAA 
CCCCAGGACA CGGACAGCGG CGTCGACACC TTCACTCGCA CCATGGACAA CAGGCCAAAA 
TATCAGCACA ACAACATCAA CGCCATTCCC AAAGCCGTCC CAGTCAGCCC CAGTGCACTG 
GATGATGATG AAGAAGATGA GGGACACACA GTTGTGGCCA CGGCGCGGGG CATCTTTAAC 
TGTAATGGCG GGGTGTTGAG CTCTATAGAG ACGGGCGTCA GCATCATTAT CCCACAGGGA 
GCCATTCCTG ATAGTGTGGA GCAGGAGATC TACTTCAAAG TGTGCCGGGA CAACAGCATC 
CTGCCTCCTC TGGATAAGGA GAAAGGTGAA ACTCTGCTGA GTCCGCTGGT GATGTGTGGT 
CCTCACGGCC TGAAGTTCCT GAAGCCTGTG GAGCTGCGCT TGCCACACTG TGCGTCTATG 
ACCCCTGATG GTTGGTCTTT TGCTCTAAAA TCCTCCGACT CCTCGTCGGG TGACCCCAAA 
TGCTGGCAAA ACAAGTGTCT ACCAGGAGAC CCCAATTACC TGGTGGGCGC AAACTGTGTG 
TCTGTGCTGA TCGACCACTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp1b ( XM_017354178.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017209667.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X14, mRNA.

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CloneID	ODa57888
Clone ID Related Accession (Same CDS sequence)	XM_017354178.1
Accession Version	XM_017354178.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5328bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tight junction protein ZO-1 isoform X14
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGA CGGTAATATG GGAACAGCAC ACTGTAACTC TACACAGGGC TCCAGGGTTT 
GGCTTCGGGA TCGCCATCTC CGGCGGACGG GACAACCCTC ACTTCCAGAG CGGGGAGACG 
TCCATCGTCA TATCAGATGT GCTGAAGGGA GGCCCCGCCG AGGGCCTGCT GCAGGAGAAT 
GACAGAGTGG TGATGGTCAA CGCCGTCTCC ATGGACAATG TGGATCACGC TTACGCAGTG 
CAGCAGCTCC GCAAAAGTGG CAAGAATGCA AAAATAACCA TCAGGAGAAA GCGTAAGGTG 
CAGATCCCGT CAGGCCGGCC GGGTGAACGC GAGACTATGT CAGAGCATGA CGAGGACGAC 
GACAGCTACG AGGATGAGAT CTACGAGTCC CGGAGCGCGC GAAGCGGACC CAGCGCCTAC 
AGTGGAGGTG GAGGCGGCGG CGGAGGTGGA GGAGCCTCGG GACGGAGGAG CGGCAGAGGC 
GACAGGCTGA GTGGGAGGAG GGAGCGGGAC AGAGAGCGCA GCGGCTCCAG AGAGAGGAGT 
CTGTCCCCGC GCTCGGACCG CCACTCTGTG TCCTCCAACC TCCCCCCACG GCCAGCCAAA 
GTCACACTCG TCAAATCCCG CAAGAATGAA GCAGAATACG GGCTTCGCCT GGCCAGCCAC 
ATCTTCGTGA AGGACATTTC CCCCGAGAGC CTGGCAGCCC GAGACGGAAA CATCCAAGAG 
GGAGACGTGG TGCTTAAGAT TAATGGCACG GTAACAGAGA ATTTGTCCCT GATCGATGCT 
AAGAAGCTGA TCGAGAGGTC AAAGGGAAAG CTGAAGATGG TGGTGCAGAG AGACGAGCGG 
GCGACGCTGC TGAACATCCC CGATCTGGAC GACAGCATAC CTTCTGCTAA TGCCTCGGAC 
AGAGATGATA TTTCAGACAT CCACTCCGTG GCCTCTGATC ATTCCAACCG ATCCCACGAC 
CGGAAGAGGA GCAGCCGCTC TCGTTCTCCA GACCGCCGCT CCGAACCCTC AGACCACTCC 
AGACACTCTC CACCACAGAT CAGCAACGGC AGGGCCACAG GGCCGACGGC TCAGAGAACG 
CGGCCAGTTC ACAGGAGCCG TGATGAAGAC AGGATCTCCA AGGCCGGGCC GCTTCCTCTA 
CCTGCTAAGA TGGTGGAGGA GATGCCGAAA GAGCAGAGCG CAGCCAGAGA AACAGAGAAA 
CAGCTCCCCC CTCTGCCAGA GCCCAAACCT GTGTACGCTC AGCCGGGCCA GCCGGACGTG 
GACCTGCCTG TTAGTCCGGC AGATGCTCCT GTGCCCAGCG TCACACATGA TGACAGCATC 
TTACGGCCGA GCATGAAGCT GGTGAAGTTC AAGAAGGGCG AGAGCGTGGG CTTGAGGCTC 
GCTGGGGGGA ACGACGTTGG GATATTTGTG GCGGGAGTTC TGGAGGACAG TCCTGCTGCT 
AAAGAGGGCC TGGAAGAGGG AGACCAGATT CTCAGGGTAA ATAATGTGGA TTTTGCAAAT 
ATCATCCGAG AGGAGGCAGT GCTGTTCCTG CTGGATCTGC CCAAAGGAGA GGAGGTCACC 
ATCCTGGCCC AGAAGAAGAA AGACGTGTAC CGCCGCATTG TAGAGTCTGA CGTGGGAGAC 
TCGTTCTACA TCAGGACTCA TTTTGAATAT GAGAAGGAGT CGCCCTATGG CCTGAGCTTC 
AATAAGGGGG AGGTCTTCAG AGTCGTCGAC ACCCTTTACA ATGGCAAACT GGGCTCCTGG 
CTCGCCATCC GCATCGGCAA AAACCACCAG GAGGTGGAGC GGGGCATCAT CCCCAACAAG 
AACAGGGCTG AGCAGTTATC AAGTGTCCAG TACACTCTCC CAAAGACTCC AGGAGGGGAT 
CGCGCCGACT TCTGGAGATT CAGAGGCCTC CGCAGCTCCA AACGAAACCT GAGGAAGAGC 
AGAGAAGACC TGTCTGCTCA GCCGGTCCAA ACCAAATTCC CTGCTTATGA AAGAGTGGTG 
CTTAGAGAAG CTGGTTTCTT GAGGCCAGTG GTCATTTTCG GACCGATCGC GGATGTGGCT 
AGAGAGAAGC TGTCCAGAGA GGAGACTGAC CTCTTCGAAC TTGCAAAGAG TGAACCCAGA 
GATGCGGGCA CAGACCAGCG CAGCTCCGGA ATCATCCGTC TCCATACCAT TAAACAGATC 
ATTGACCGAG ACAAGCACGC GGTGCTGGAC ATCACCCCTA ATGCGGTGGA CCGTCTGAAC 
TACGCTCAGT GGTACCCCAT CGTGGTGTTT CTGAATCCAG ACAGCAAACA GGGCGTCAAG 
AACATGAGGA CCAGGCTCTG TCCCGAGTCC AGGAAGAGCG CCCGAAAGCT CTACGAGCGT 
GCTCTCAAAC TGAGGAAGAA CAACCATCAC CTTTTCACCA CCACTATTAA CTTGAACAAC 
ATGAATGACG GGTGGTACGG TGCTTTGAAA GAGAGCATCC AGCAGCAGCA GAACCAGTTG 
GTCTGGGTCT CAGAGGGCAA GGCCGACGGC GCTCCTGACG ATGACCTTGA CATCCATGAC 
GACCGCCTCT CCTACCTCTC GGCTCCGGGC AGCGAGTACA GCATGTACAG CACCGACAGC 
CGCCACACGT CCGACTATGA GGACACCGAC ACGGAGGGTG GTGCCTACAC CGACCAGGAG 
CTGGACGAGA CCATGAACGA TGAGGTGGGC CTGCCTAGCG AGCCTGCCAT CACACGCTCA 
TCTGAGCCTG TGCGAGAGGA CCCGCCAGTC ATTCAGGACG CAGCTGGATA CCCCAGCTAC 
CAGCATGCGG TACCGCAACC TGAAGCGGTG AGCCGCATTG ACCCGGCCGG GTTTAAGATG 
GCAGCGCCGC AGCAGCAAGC TGAGGCTGCT CTGGCCTCGC CGCCCCCTCC CTCTGCAGCG 
GTCCAGCCCA CCGCAACACT AGCGGGTACG AACCCCGAGG AGCCGCCTGG CACCCCTCAG 
GCCGACTCCC TTAACAGCCC CGTCCCTGTT CCCGACCCCC AGCCCGAACC TGAGCTCGCT 
CAGCCCCCAA CACACGACCC CCACCAGTCG CTTCCTCCTG GCCCAGATCC AAAGATGTAT 
AAAAAGGATC TGTACAGCAT GGACGAGCCG GTGCGAGTGA CCCACGGCGT GAAGCCTCCC 
CCTCCCCAGC AGCAGCAGCA GCCTTTAGGC CCCCCAACCT CGCTCTCCTA CAGCCACCAG 
CCTGTCTACC AGGACCAACA GCCATACCGC CAGTACGACC ACCCGCCTTA TGGCTATGAC 
GGTGGCGGCT ACGCACAACC AAAGCCTCAC AACTACGACC CGCACCTGCA CTACGACAAC 
CGTGTGCCTC ACTATAACGA GCAGTGGCCC ACGTACGACC AGCAGCAGCA GCAGCAGACT 
TCCCCCCAGC CGCCACCTGC GTCCGGCTTC CCTCAGGGCC ACCAGCCTCC GCCTCCACCA 
CCGATGGGAT ATGAGCCACG CTCCCCATAC GAGGACGGAC CTAACAGGGA TTTCAGCCCT 
CCTCAGTCCC AGTATGACGG TGTGTCCCCA ATGGGCTACG ATCACCGGCC GCGGCACCCC 
AAACCTGCAC CGGTCCGATA TGAAGAGCCT CCACCTCCAC CTCCTCCGGT GTCCTACGAT 
GCACGGTCAC CTTTCGAACC CGAGCCGCAT GCTTTCCCAG GCAACACACA TCGCTCGCCA 
GACCCACCGA AACAGTATTA TGGAGACGCC GCTATGAGGC CCTTGTACAA CCCTGGACCT 
CCGAACAGAG TGTACAAACA TGAGCCGGTG AACTCTGAGC CTCCTGTACC GCCTCCCAAA 
CCTGAATCTG TCCTGTCTCC AGGAGAACCG CCCCATTCTG CAGCTTCCAA ACCATTGCCG 
CCTCCTCCCA GAGATGATGA CGATGATGAT GACGATGATG ATGACCCTGC CATGAAGCCC 
CAGTCTGTGC TCAACAGGGT CAAAATGTTC GAAAACAAAC GCTCTGTGTC GGTAGACCGA 
GCCAAGGACG CACCTGACGT CACTGGAATT AGGCCCACAG ATCTCCCCAA ACCTGCGAGC 
ACCCCTGGAC CTGTGCTCAA AGCCAACTCT CTCAGCAACC TGGAGCAGGA CAAACCCTCC 
TACAGAGCTC CAGAGCCGCA GAAACCTCAG ACGCGTGTTG GGGACGATGT GGTTCGCTCC 
AACCACTACG ACCCTGATGA TGATGAAGAG TACTACAGAA AACAGCTCTC ATTTTTCGAC 
CGCCGCAGTT TCGACAGTAA GGTCATGAAT CCACCCAGCG CTGGAGTAAA CCGCTTCCAT 
GAGCCGCCCA AACCACCCCA GACACAGATC GCTTATCCTT TCGCTAGGAC GGAGTCTGTG 
GAGAAGGTGA GTCCTGTGGA CAAGAGATAC GAGCCTCTGC CGCCAGTCAA CCCTTCACCT 
GCTCCATACA GCCAGAACAC ACCTGTCGCT CCGCCCACAT CCCTGCCCAA ACTCACCAAC 
ATCGAGGTGA ACTCTCTGCC AGATCCTCAC AGCTCTCCCA AAGCCAAACC GGACCTGTCT 
GCTCTGAGAG CTCCGACCCG GGACGAGCCC ATCCAGAGCA GCTACTTGCC CCCCAAGTCC 
ACCATCAATG GCATGGATGC TCCTCCCAAA ACACTCGGCG TGCCGACCAG CTACAACCGC 
TACGTCCCCA AGCCTTACAC CAGCTCGGCG CGACCCTTCG AGCGCAAGTT CGAGAGTCCG 
AAATTCAACC ACAACCTGCT TCCCAACGAC ACGCAGTCCA AACCCAGTGT CAACAACAAC 
CTGAAGCCTC AGATCTCCCC ACAACCCCAG GACACGGACA GCGGCGTCGA CACCTTCACT 
CGCACCATGG ACAACAGGCC AAAATATCAG CACAACAACA TCAACGCCAT TCCCAAAGCC 
GTCCCAGTCA GCCCCAGTGC ACTGGATGAT GATGAAGAAG ATGAGGGACA CACAGTTGTG 
GCCACGGCGC GGGGCATCTT TAACTGTAAT GGCGGGGTGT TGAGCTCTAT AGAGACGGGC 
GTCAGCATCA TTATCCCACA GGGAGCCATT CCTGATAGTG TGGAGCAGGA GATCTACTTC 
AAAGTGTGCC GGGACAACAG CATCCTGCCT CCTCTGGATA AGGAGAAAGG TGAAACTCTG 
CTGAGTCCGC TGGTGATGTG TGGTCCTCAC GGCCTGAAGT TCCTGAAGCC TGTGGAGCTG 
CGCTTGCCAC ACTGTGCGTC TATGACCCCT GATGGTTGGT CTTTTGCTCT AAAATCCTCC 
GACTCCTCGT CGGGTGACCC CAAATGCTGG CAAAACAAGT GTCTACCAGG AGACCCCAAT 
TACCTGGTGG GCGCAAACTG TGTGTCTGTG CTGATCGACC ACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209667.1
CDS	191..5518
Translation	MDETVIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGLLQENDRVVMVNAVSMDNVDHAYAVQQLRKSGKNAKITIRRKRKVQIPSGRPGERETMSEHDEDDDSYEDEIYESRSARSGPSAYSGGGGGGGGGGASGRRSGRGDRLSGRRERDRERSGSRERSLSPRSDRHSVSSNLPPRPAKVTLVKSRKNEAEYGLRLASHIFVKDISPESLAARDGNIQEGDVVLKINGTVTENLSLIDAKKLIERSKGKLKMVVQRDERATLLNIPDLDDSIPSANASDRDDISDIHSVASDHSNRSHDRKRSSRSRSPDRRSEPSDHSRHSPPQISNGRATGPTAQRTRPVHRSRDEDRISKAGPLPLPAKMVEEMPKEQSAARETEKQLPPLPEPKPVYAQPGQPDVDLPVSPADAPVPSVTHDDSILRPSMKLVKFKKGESVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFANIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHQEVERGIIPNKNRAEQLSSVQYTLPKTPGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVVIFGPIADVAREKLSREETDLFELAKSEPRDAGTDQRSSGIIRLHTIKQIIDRDKHAVLDITPNAVDRLNYAQWYPIVVFLNPDSKQGVKNMRTRLCPESRKSARKLYERALKLRKNNHHLFTTTINLNNMNDGWYGALKESIQQQQNQLVWVSEGKADGAPDDDLDIHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETMNDEVGLPSEPAITRSSEPVREDPPVIQDAAGYPSYQHAVPQPEAVSRIDPAGFKMAAPQQQAEAALASPPPPSAAVQPTATLAGTNPEEPPGTPQADSLNSPVPVPDPQPEPELAQPPTHDPHQSLPPGPDPKMYKKDLYSMDEPVRVTHGVKPPPPQQQQQPLGPPTSLSYSHQPVYQDQQPYRQYDHPPYGYDGGGYAQPKPHNYDPHLHYDNRVPHYNEQWPTYDQQQQQQTSPQPPPASGFPQGHQPPPPPPMGYEPRSPYEDGPNRDFSPPQSQYDGVSPMGYDHRPRHPKPAPVRYEEPPPPPPPVSYDARSPFEPEPHAFPGNTHRSPDPPKQYYGDAAMRPLYNPGPPNRVYKHEPVNSEPPVPPPKPESVLSPGEPPHSAASKPLPPPPRDDDDDDDDDDDPAMKPQSVLNRVKMFENKRSVSVDRAKDAPDVTGIRPTDLPKPASTPGPVLKANSLSNLEQDKPSYRAPEPQKPQTRVGDDVVRSNHYDPDDDEEYYRKQLSFFDRRSFDSKVMNPPSAGVNRFHEPPKPPQTQIAYPFARTESVEKVSPVDKRYEPLPPVNPSPAPYSQNTPVAPPTSLPKLTNIEVNSLPDPHSSPKAKPDLSALRAPTRDEPIQSSYLPPKSTINGMDAPPKTLGVPTSYNRYVPKPYTSSARPFERKFESPKFNHNLLPNDTQSKPSVNNNLKPQISPQPQDTDSGVDTFTRTMDNRPKYQHNNINAIPKAVPVSPSALDDDEEDEGHTVVATARGIFNCNGGVLSSIETGVSIIIPQGAIPDSVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKCWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_017354178.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tight junction protein 1b (tjp1b), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017354178.1

ORF Insert Sequence:

ATGGATGAGA CGGTAATATG GGAACAGCAC ACTGTAACTC TACACAGGGC TCCAGGGTTT 
GGCTTCGGGA TCGCCATCTC CGGCGGACGG GACAACCCTC ACTTCCAGAG CGGGGAGACG 
TCCATCGTCA TATCAGATGT GCTGAAGGGA GGCCCCGCCG AGGGCCTGCT GCAGGAGAAT 
GACAGAGTGG TGATGGTCAA CGCCGTCTCC ATGGACAATG TGGATCACGC TTACGCAGTG 
CAGCAGCTCC GCAAAAGTGG CAAGAATGCA AAAATAACCA TCAGGAGAAA GCGTAAGGTG 
CAGATCCCGT CAGGCCGGCC GGGTGAACGC GAGACTATGT CAGAGCATGA CGAGGACGAC 
GACAGCTACG AGGATGAGAT CTACGAGTCC CGGAGCGCGC GAAGCGGACC CAGCGCCTAC 
AGTGGAGGTG GAGGCGGCGG CGGAGGTGGA GGAGCCTCGG GACGGAGGAG CGGCAGAGGC 
GACAGGCTGA GTGGGAGGAG GGAGCGGGAC AGAGAGCGCA GCGGCTCCAG AGAGAGGAGT 
CTGTCCCCGC GCTCGGACCG CCACTCTGTG TCCTCCAACC TCCCCCCACG GCCAGCCAAA 
GTCACACTCG TCAAATCCCG CAAGAATGAA GCAGAATACG GGCTTCGCCT GGCCAGCCAC 
ATCTTCGTGA AGGACATTTC CCCCGAGAGC CTGGCAGCCC GAGACGGAAA CATCCAAGAG 
GGAGACGTGG TGCTTAAGAT TAATGGCACG GTAACAGAGA ATTTGTCCCT GATCGATGCT 
AAGAAGCTGA TCGAGAGGTC AAAGGGAAAG CTGAAGATGG TGGTGCAGAG AGACGAGCGG 
GCGACGCTGC TGAACATCCC CGATCTGGAC GACAGCATAC CTTCTGCTAA TGCCTCGGAC 
AGAGATGATA TTTCAGACAT CCACTCCGTG GCCTCTGATC ATTCCAACCG ATCCCACGAC 
CGGAAGAGGA GCAGCCGCTC TCGTTCTCCA GACCGCCGCT CCGAACCCTC AGACCACTCC 
AGACACTCTC CACCACAGAT CAGCAACGGC AGGGCCACAG GGCCGACGGC TCAGAGAACG 
CGGCCAGTTC ACAGGAGCCG TGATGAAGAC AGGATCTCCA AGGCCGGGCC GCTTCCTCTA 
CCTGCTAAGA TGGTGGAGGA GATGCCGAAA GAGCAGAGCG CAGCCAGAGA AACAGAGAAA 
CAGCTCCCCC CTCTGCCAGA GCCCAAACCT GTGTACGCTC AGCCGGGCCA GCCGGACGTG 
GACCTGCCTG TTAGTCCGGC AGATGCTCCT GTGCCCAGCG TCACACATGA TGACAGCATC 
TTACGGCCGA GCATGAAGCT GGTGAAGTTC AAGAAGGGCG AGAGCGTGGG CTTGAGGCTC 
GCTGGGGGGA ACGACGTTGG GATATTTGTG GCGGGAGTTC TGGAGGACAG TCCTGCTGCT 
AAAGAGGGCC TGGAAGAGGG AGACCAGATT CTCAGGGTAA ATAATGTGGA TTTTGCAAAT 
ATCATCCGAG AGGAGGCAGT GCTGTTCCTG CTGGATCTGC CCAAAGGAGA GGAGGTCACC 
ATCCTGGCCC AGAAGAAGAA AGACGTGTAC CGCCGCATTG TAGAGTCTGA CGTGGGAGAC 
TCGTTCTACA TCAGGACTCA TTTTGAATAT GAGAAGGAGT CGCCCTATGG CCTGAGCTTC 
AATAAGGGGG AGGTCTTCAG AGTCGTCGAC ACCCTTTACA ATGGCAAACT GGGCTCCTGG 
CTCGCCATCC GCATCGGCAA AAACCACCAG GAGGTGGAGC GGGGCATCAT CCCCAACAAG 
AACAGGGCTG AGCAGTTATC AAGTGTCCAG TACACTCTCC CAAAGACTCC AGGAGGGGAT 
CGCGCCGACT TCTGGAGATT CAGAGGCCTC CGCAGCTCCA AACGAAACCT GAGGAAGAGC 
AGAGAAGACC TGTCTGCTCA GCCGGTCCAA ACCAAATTCC CTGCTTATGA AAGAGTGGTG 
CTTAGAGAAG CTGGTTTCTT GAGGCCAGTG GTCATTTTCG GACCGATCGC GGATGTGGCT 
AGAGAGAAGC TGTCCAGAGA GGAGACTGAC CTCTTCGAAC TTGCAAAGAG TGAACCCAGA 
GATGCGGGCA CAGACCAGCG CAGCTCCGGA ATCATCCGTC TCCATACCAT TAAACAGATC 
ATTGACCGAG ACAAGCACGC GGTGCTGGAC ATCACCCCTA ATGCGGTGGA CCGTCTGAAC 
TACGCTCAGT GGTACCCCAT CGTGGTGTTT CTGAATCCAG ACAGCAAACA GGGCGTCAAG 
AACATGAGGA CCAGGCTCTG TCCCGAGTCC AGGAAGAGCG CCCGAAAGCT CTACGAGCGT 
GCTCTCAAAC TGAGGAAGAA CAACCATCAC CTTTTCACCA CCACTATTAA CTTGAACAAC 
ATGAATGACG GGTGGTACGG TGCTTTGAAA GAGAGCATCC AGCAGCAGCA GAACCAGTTG 
GTCTGGGTCT CAGAGGGCAA GGCCGACGGC GCTCCTGACG ATGACCTTGA CATCCATGAC 
GACCGCCTCT CCTACCTCTC GGCTCCGGGC AGCGAGTACA GCATGTACAG CACCGACAGC 
CGCCACACGT CCGACTATGA GGACACCGAC ACGGAGGGTG GTGCCTACAC CGACCAGGAG 
CTGGACGAGA CCATGAACGA TGAGGTGGGC CTGCCTAGCG AGCCTGCCAT CACACGCTCA 
TCTGAGCCTG TGCGAGAGGA CCCGCCAGTC ATTCAGGACG CAGCTGGATA CCCCAGCTAC 
CAGCATGCGG TACCGCAACC TGAAGCGGTG AGCCGCATTG ACCCGGCCGG GTTTAAGATG 
GCAGCGCCGC AGCAGCAAGC TGAGGCTGCT CTGGCCTCGC CGCCCCCTCC CTCTGCAGCG 
GTCCAGCCCA CCGCAACACT AGCGGGTACG AACCCCGAGG AGCCGCCTGG CACCCCTCAG 
GCCGACTCCC TTAACAGCCC CGTCCCTGTT CCCGACCCCC AGCCCGAACC TGAGCTCGCT 
CAGCCCCCAA CACACGACCC CCACCAGTCG CTTCCTCCTG GCCCAGATCC AAAGATGTAT 
AAAAAGGATC TGTACAGCAT GGACGAGCCG GTGCGAGTGA CCCACGGCGT GAAGCCTCCC 
CCTCCCCAGC AGCAGCAGCA GCCTTTAGGC CCCCCAACCT CGCTCTCCTA CAGCCACCAG 
CCTGTCTACC AGGACCAACA GCCATACCGC CAGTACGACC ACCCGCCTTA TGGCTATGAC 
GGTGGCGGCT ACGCACAACC AAAGCCTCAC AACTACGACC CGCACCTGCA CTACGACAAC 
CGTGTGCCTC ACTATAACGA GCAGTGGCCC ACGTACGACC AGCAGCAGCA GCAGCAGACT 
TCCCCCCAGC CGCCACCTGC GTCCGGCTTC CCTCAGGGCC ACCAGCCTCC GCCTCCACCA 
CCGATGGGAT ATGAGCCACG CTCCCCATAC GAGGACGGAC CTAACAGGGA TTTCAGCCCT 
CCTCAGTCCC AGTATGACGG TGTGTCCCCA ATGGGCTACG ATCACCGGCC GCGGCACCCC 
AAACCTGCAC CGGTCCGATA TGAAGAGCCT CCACCTCCAC CTCCTCCGGT GTCCTACGAT 
GCACGGTCAC CTTTCGAACC CGAGCCGCAT GCTTTCCCAG GCAACACACA TCGCTCGCCA 
GACCCACCGA AACAGTATTA TGGAGACGCC GCTATGAGGC CCTTGTACAA CCCTGGACCT 
CCGAACAGAG TGTACAAACA TGAGCCGGTG AACTCTGAGC CTCCTGTACC GCCTCCCAAA 
CCTGAATCTG TCCTGTCTCC AGGAGAACCG CCCCATTCTG CAGCTTCCAA ACCATTGCCG 
CCTCCTCCCA GAGATGATGA CGATGATGAT GACGATGATG ATGACCCTGC CATGAAGCCC 
CAGTCTGTGC TCAACAGGGT CAAAATGTTC GAAAACAAAC GCTCTGTGTC GGTAGACCGA 
GCCAAGGACG CACCTGACGT CACTGGAATT AGGCCCACAG ATCTCCCCAA ACCTGCGAGC 
ACCCCTGGAC CTGTGCTCAA AGCCAACTCT CTCAGCAACC TGGAGCAGGA CAAACCCTCC 
TACAGAGCTC CAGAGCCGCA GAAACCTCAG ACGCGTGTTG GGGACGATGT GGTTCGCTCC 
AACCACTACG ACCCTGATGA TGATGAAGAG TACTACAGAA AACAGCTCTC ATTTTTCGAC 
CGCCGCAGTT TCGACAGTAA GGTCATGAAT CCACCCAGCG CTGGAGTAAA CCGCTTCCAT 
GAGCCGCCCA AACCACCCCA GACACAGATC GCTTATCCTT TCGCTAGGAC GGAGTCTGTG 
GAGAAGGTGA GTCCTGTGGA CAAGAGATAC GAGCCTCTGC CGCCAGTCAA CCCTTCACCT 
GCTCCATACA GCCAGAACAC ACCTGTCGCT CCGCCCACAT CCCTGCCCAA ACTCACCAAC 
ATCGAGGTGA ACTCTCTGCC AGATCCTCAC AGCTCTCCCA AAGCCAAACC GGACCTGTCT 
GCTCTGAGAG CTCCGACCCG GGACGAGCCC ATCCAGAGCA GCTACTTGCC CCCCAAGTCC 
ACCATCAATG GCATGGATGC TCCTCCCAAA ACACTCGGCG TGCCGACCAG CTACAACCGC 
TACGTCCCCA AGCCTTACAC CAGCTCGGCG CGACCCTTCG AGCGCAAGTT CGAGAGTCCG 
AAATTCAACC ACAACCTGCT TCCCAACGAC ACGCAGTCCA AACCCAGTGT CAACAACAAC 
CTGAAGCCTC AGATCTCCCC ACAACCCCAG GACACGGACA GCGGCGTCGA CACCTTCACT 
CGCACCATGG ACAACAGGCC AAAATATCAG CACAACAACA TCAACGCCAT TCCCAAAGCC 
GTCCCAGTCA GCCCCAGTGC ACTGGATGAT GATGAAGAAG ATGAGGGACA CACAGTTGTG 
GCCACGGCGC GGGGCATCTT TAACTGTAAT GGCGGGGTGT TGAGCTCTAT AGAGACGGGC 
GTCAGCATCA TTATCCCACA GGGAGCCATT CCTGATAGTG TGGAGCAGGA GATCTACTTC 
AAAGTGTGCC GGGACAACAG CATCCTGCCT CCTCTGGATA AGGAGAAAGG TGAAACTCTG 
CTGAGTCCGC TGGTGATGTG TGGTCCTCAC GGCCTGAAGT TCCTGAAGCC TGTGGAGCTG 
CGCTTGCCAC ACTGTGCGTC TATGACCCCT GATGGTTGGT CTTTTGCTCT AAAATCCTCC 
GACTCCTCGT CGGGTGACCC CAAATGCTGG CAAAACAAGT GTCTACCAGG AGACCCCAAT 
TACCTGGTGG GCGCAAACTG TGTGTCTGTG CTGATCGACC ACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	tjp1b
Entrez Gene ID	565459
Full Name	tight junction protein 1b
Synonyms	si:dkey-33l14.1
General protein information	Preferred Names tight junction protein 1b Names tight junction protein ZO-1 tjp1.2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

Homo sapiens (human)	TJP1	NP_003248.3
	TJP1	XP_002804738.1
Canis lupus familiaris (dog)	TJP1	NP_001003140.1
Rattus norvegicus (Norway rat)	Tjp1	NP_001099736.1
Danio rerio (zebrafish)	LOC565459	XP_003201509.2
Bos taurus (cattle)	TJP1	XP_002696696.3
Gallus gallus (chicken)	TJP1	XP_413773.4
Danio rerio (zebrafish)	tjp1a	XP_005168990.1
Pan troglodytes (chimpanzee)	TJP1	XP_001163120.1
Mus musculus (house mouse)	Tjp1	NP_033412.2
Xenopus tropicalis (tropical clawed frog)	tjp1	XP_004917990.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

tjp1b cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

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