POL1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	POL1
Entrez Gene ID	855621
Full Name	DNA-directed DNA polymerase alpha catalytic subunit POL1
Synonyms	CDC17,CRT5,HPR3
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001182940.3	NP_014297.3	DNA-directed DNA polymerase alpha catalytic subunit POL1

Pathways from BioSystems

Homologies

	POLA1	XP_001091195.1
Gallus gallus (chicken)	POLA1	XP_416792.3
Eremothecium gossypii	AGOS_AER381C	NP_985237.1
Homo sapiens (human)	POLA1	NP_058633.2
Rattus norvegicus (Norway rat)	Pola1	NP_445931.1
Schizosaccharomyces pombe (fission yeast)	pol1	NP_594186.1
Pan troglodytes (chimpanzee)	POLA1	XP_003317445.2
Mus musculus (house mouse)	Pola1	NP_032918.1
Danio rerio (zebrafish)	pola1	XP_005162809.1
Drosophila melanogaster (fruit fly)	DNApol-alpha180	NP_536736.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001929	XP_321134.5
Caenorhabditis elegans (roundworm)	Y47D3A.29	NP_001255109.1
Magnaporthe oryzae (rice blast fungus)	MGG_06397	XP_003717174.1
Neurospora crassa	NCU07870	XP_963131.2
Arabidopsis thaliana (thale cress)	ICU2	NP_201511.2
Xenopus tropicalis (tropical clawed frog)	pola1	XP_004911812.1
Bos taurus (cattle)	POLA1	NP_001192994.1
Saccharomyces cerevisiae (baker's yeast)	POL1	NP_014297.3
Kluyveromyces lactis	KLLA0C13255g	XP_452791.1
Canis lupus familiaris (dog)	POLA1	XP_005641271.1
Oryza sativa (rice)	Os01g0868300	NP_001044920.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.
Philippsen P, Kleine K, P?hlmann R, D?sterh?ft A, Hamberg K, Hegemann JH, Obermaier B, Urrestarazu LA, Aert R, Albermann K, Altmann R, Andr? B, Baladron V, Ballesta JP, B?cam AM, Beinhauer J, Boskovic J, Buitrago MJ, Bussereau F, Coster F, Crouzet M, D'Angelo M, Dal Pero F, De Antoni A, Del Rey F, Doignon F, Domdey H, Dubois E, Fiedler T, Fleig U, Floeth M, Fritz C, Gaillardin C, Garcia-Cantalejo JM, Glansdorff NN, Goffeau A, Gueldener U, Herbert C, Heumann K, Heuss-Neitzel D, Hilbert H, Hinni K, Iraqui Houssaini I, Jacquet M, Jimenez A, Jonniaux JL, Karpfinger L, Lanfranchi G, Lepingle A, Levesque H, Lyck R, Maftahi M, Mallet L, Maurer KC, Messenguy F, Mewes HW, M?sti D, Nasr F, Nicaud JM, Niedenthal RK, Pandolfo D, Pi?rard A, Piravandi E, Planta RJ, Pohl TM, Purnelle B, Rebischung C, Remacha M, Revuelta JL, Rinke M, Saiz JE, Sartorello F, Scherens B, Sen-Gupta M, Soler-Mira A, Urbanus JH, Valle G, Van Dyck L, Verhasselt P, Vierendeels F, Vissers S, Voet M, Volckaert G, Wach A, Wambutt R, Wedler H, Zollner A, Hani J
Nature387(6632 Suppl)93-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

An intact Mcm10 coiled-coil interaction surface is important for origin melting, helicase assembly and the recruitment of Pol-alpha to Mcm2-7.
Title: An intact Mcm10 coiled-coil interaction surface is important for origin melting, helicase assembly and the recruitment of Pol-? to Mcm2-7.

We conclude that Fob1 and Pol I make independent contributions to establishment of silencing, though Pol I also reinforces Fob1-dependent silencing.
Title: RNA Polymerase I and Fob1 contributions to transcriptional silencing at the yeast rDNA locus.

Collectively, these data demonstrate a novel role for Ccr4-Not in Pol I transcriptional regulation that is required for bridging mTORC1 signaling to ribosomal RNA synthesis.
Title: Ccr4-not regulates RNA polymerase I transcription and couples nutrient signaling to the control of ribosomal RNA biogenesis.

Data indicate that a defective DNA polymerase alpha (Polalpha)/DNA primase/chromatin-binding protein Ctf4 complex causes unscheduled strand annealing and fork reversal.
Title: Error-free DNA damage tolerance and sister chromatid proximity during DNA replication rely on the Pol?/Primase/Ctf4 Complex.

Data suggest that DNA-directed DNA polymerase Pols alpha and delta are the primary lagging-strand replicases and Pol epsilon is primarily a leading-strand replicase.
Title: Heterogeneous polymerase fidelity and mismatch repair bias genome variation and composition.

The following POL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi04530	NM_001182940.3 Latest version!	Saccharomyces cerevisiae S288C DNA-directed DNA polymerase alpha catalytic subunit POL1 (POL1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

POL1 ( NM_001182940.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_014297.3 Saccharomyces cerevisiae S288C DNA-directed DNA polymerase alpha catalytic subunit POL1 (POL1), partial mRNA.

Price & Availability↑

CloneID	OSi04530
Clone ID Related Accession (Same CDS sequence)	NM_001182940.3
Accession Version	NM_001182940.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4407bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	DNA-directed DNA polymerase alpha catalytic subunit POL1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001146). On Jul 30, 2012 this sequence version replaced NM_001182940.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCTTCTA AAAGTGAAAA ATTAGAAAAA TTGAGAAAGC TGCAAGCCGC TCGAAATGGT 
ACATCCATCG ATGATTACGA AGGTGACGAA TCAGATGGTG ACAGAATATA CGACGAAATC 
GACGAAAAAG AATACAGGGC CAGAAAGCGC CAGGAATTGC TGCATGACGA TTTTGTTGTA 
GATGACGATG GTGTAGGCTA CGTCGATCGT GGTGTCGAAG AAGATTGGAG GGAAGTTGAT 
AACAGTTCAA GTGATGAAGA CACTGGAAAC CTTGCTAGCA AGGATTCTAA AAGGAAGAAG 
AATATAAAAA GGGAGAAAGA CCACCAGATT ACAGATATGC TGCGAACTCA ACATTCCAAA 
TCAACACTGC TAGCACATGC AAAAAAATCC CAAAAAAAAA GTATCCCCAT CGATAATTTT 
GATGACATTC TTGGTGAGTT TGAATCTGGT GAAGTAGAAA AACCCAATAT TTTACTGCCA 
TCCAAATTAA GGGAAAATTT AAATTCTTCG CCGACTAGTG AATTTAAATC GTCAATAAAG 
AGAGTTAACG GAAACGATGA GTCAAGTCAT GATGCCGGTA TTTCTAAAAA AGTCAAGATT 
GATCCAGATT CAAGTACTGA TAAATATTTA GAAATCGAAT CATCACCGTT AAAATTACAA 
AGCAGGAAAC TGCGCTACGC AAATGATGTA CAGGATTTAT TGGATGATGT GGAAAATTCC 
CCAGTAGTAG CCACAAAGAG ACAGAATGTA CTTCAAGATA CGTTACTAGC AAATCCACCA 
TCCGCTCAAA GTTTGGCTGA TGAAGAAGAT GATGAAGATA GTGATGAAGA TATTATTCTG 
AAAAGAAGAA CTATGAGAAG TGTGACAACA ACTAGACGCG TGAATATAGA CTCAAGAAGT 
AACCCTTCAA CTTCACCGTT TGTCACAGCA CCCGGAACGC CAATCGGTAT TAAAGGACTA 
ACTCCAAGTA AATCTTTGCA GAGCAACACC GATGTTGCTA CACTTGCGGT TAATGTCAAA 
AAGGAAGATG TGGTAGATCC GGAAACTGAT ACTTTCCAAA TGTTTTGGTT GGATTACTGT 
GAAGTGAACA ACACTTTGAT TTTATTTGGT AAGGTTAAAC TAAAGGATGA TAATTGTGTG 
TCAGCCATGG TTCAAATCAA CGGTCTCTGT AGAGAGCTAT TTTTCCTTCC AAGAGAGGGT 
AAAACACCTA CCGATATACA CGAGGAAATA ATTCCGTTAC TGATGGATAA ATATGGACTG 
GATAATATCC GCGCTAAACC TCAAAAAATG AAATATTCGT TTGAGCTTCC TGACATTCCA 
TCTGAAAGTG ATTATTTGAA AGTCTTACTT CCATACCAAA CACCGAAGTC CAGTCGAGAC 
ACCATTCCAT CGGATTTATC TAGCGATACC TTTTATCATG TTTTTGGTGG GAATTCCAAT 
ATTTTTGAAA GTTTTGTTAT TCAAAATAGG ATTATGGGAC CATGCTGGTT AGATATAAAG 
GGAGCAGATT TCAATTCAAT TCGTAATGCT TCTCACTGTG CGGTTGAAGT TTCGGTAGAT 
AAGCCTCAGA ATATCACGCC TACTACAACA AAGACAATGC CTAATCTGAG ATGTCTATCT 
TTATCGATTC AGACGTTAAT GAATCCCAAG GAAAATAAGC AAGAGATTGT TTCGATAACG 
TTATCAGCAT ATAGAAATAT CTCGTTGGAT TCACCGATAC CTGAAAATAT AAAACCAGAC 
GACTTGTGTA CTTTAGTAAG GCCGCCTCAG TCAACTAGTT TCCCATTAGG TTTAGCTGCA 
CTGGCAAAAC AGAAACTACC CGGTAGAGTC AGGTTGTTCA ACAACGAAAA GGCCATGCTA 
TCTTGTTTTT GTGCTATGTT AAAAGTGGAA GACCCAGATG TTATCATAGG CCATCGTTTG 
CAAAATGTCT ACTTAGATGT TTTAGCTCAT AGAATGCATG ATCTCAACAT TCCAACGTTC 
AGCTCTATTG GCCGTCGTTT AAGAAGGACT TGGCCTGAAA AATTCGGTAG AGGAAATTCG 
AATATGAACC ATTTTTTTAT TAGTGACATT TGCTCTGGTA GGCTGATATG TGATATCGCC 
AATGAAATGG GTCAATCATT AACACCCAAA TGTCAAAGTT GGGATCTTTC AGAGATGTAT 
CAAGTTACAT GTGAAAAGGA GCATAAGCCG TTAGATATTG ATTATCAAAA TCCACAATAC 
CAAAATGATG TAAATAGTAT GACAATGGCC CTACAAGAGA ATATTACTAA TTGTATGATT 
TCTGCTGAGG TGTCTTATAG AATTCAATTA TTAACCTTGA CTAAACAGTT GACAAATTTG 
GCTGGTAATG CATGGGCCCA AACGCTAGGC GGTACAAGAG CTGGTAGAAA TGAGTATATC 
TTACTACATG AATTTTCAAG AAATGGTTTT ATTGTTCCTG ACAAAGAAGG CAATAGAAGT 
AGAGCTCAGA AACAAAGACA AAATGAAGAA AATGCTGATG CACCAGTTAA TTCTAAAAAG 
GCAAAATATC AGGGTGGTTT AGTTTTTGAA CCTGAGAAAG GTCTTCATAA GAATTACGTT 
TTAGTCATGG ACTTTAATTC TTTGTATCCA TCTATTATCC AGGAATTTAA TATATGTTTT 
ACCACCGTTG ATAGAAATAA GGAAGATATC GATGAGTTAC CAAGCGTACC TCCTTCAGAG 
GTGGATCAGG GTGTTTTACC AAGGTTATTA GCTAATCTGG TGGATCGTCG ACGTGAAGTT 
AAGAAGGTGA TGAAAACTGA AACTGATCCC CATAAGCGAG TTCAATGTGA TATTCGTCAA 
CAAGCTCTAA AATTGACTGC CAATTCTATG TATGGTTGTT TGGGTTATGT TAACAGTAGA 
TTTTACGCAA AGCCGCTCGC CATGTTAGTC ACTAATAAGG GTCGTGAGAT TTTAATGAAT 
ACAAGACAAC TAGCTGAAAG TATGAATCTT CTTGTAGTTT ATGGTGATAC AGATTCGGTC 
ATGATAGATA CCGGTTGTGA TAATTATGCG GATGCAATTA AAATTGGCTT GGGATTTAAA 
AGGCTAGTAA ATGAGCGCTA TAGATTATTG GAGATTGATA TTGATAATGT TTTTAAGAAG 
TTACTATTAC ATGCCAAGAA AAAGTACGCT GCTTTGACTG TAAATTTGGA CAAAAATGGT 
AATGGAACTA CTGTTCTAGA AGTTAAAGGG TTGGACATGA AGCGTCGTGA ATTTTGTCCA 
CTTTCGAGGG ACGTTTCTAT ACATGTTTTA AACACCATCC TGTCAGATAA GGACCCAGAA 
GAAGCATTGC AAGAAGTGTA TGATTACTTA GAAGACATCA GAATAAAAGT GGAAACCAAT 
AACATTAGAA TTGATAAATA TAAGATCAAT ATGAAGCTTT CAAAAGATCC CAAGGCCTAC 
CCAGGTGGTA AAAACATGCC TGCAGTCCAA GTAGCTCTAA GAATGCGTAA GGCTGGTAGA 
GTTGTTAAAG CTGGCTCTGT CATCACTTTT GTGATCACAA AGCAGGATGA AATAGACAAT 
GCAGCGGATA CGCCGGCTCT TTCTGTGGCT GAACGTGCCC ATGCATTGAA TGAGGTAATG 
ATTAAAAGTA ACAATTTGAT ACCTGATCCA CAATATTATC TCGAGAAACA AATATTCGCG 
CCGGTAGAAA GGTTGTTAGA AAGAATTGAT AGCTTCAACG TGGTGCGTTT GAGTGAAGCG 
CTTGGTTTAG ATAGTAAAAA GTATTTTAGA AGAGAGGGCG GTAATAACAA TGGAGAAGAT 
ATTAATAATT TGCAACCCTT AGAAACAACA ATTACAGACG TTGAAAGGTT TAAGGATACT 
GTAACATTAG AATTAAGTTG CCCATCATGC GATAAAAGGT TTCCATTTGG TGGTATTGTA 
TCTTCAAATT ACTATCGCGT GTCATATAAT GGTTTACAGT GCAAGCATTG TGAGCAACTT 
TTTACTCCTC TTCAATTAAC TAGCCAAATA GAGCATTCTA TAAGGGCACA CATTTCCTTA 
TATTACGCAG GGTGGTTACA GTGTGATGAC AGCACATGCG GTATAGTTAC AAGACAAGTC 
TCCGTTTTTG GTAAGCGTTG TTTGAATGAC GGCTGCACGG GTGTCATGAG ATACAAATAC 
AGTGACAAGC AATTGTACAA TCAACTTTTA TATTTCGATT CTTTATTCGA TTGTGAAAAA 
AATAAAAAGC AAGAATTGAA GCCAATATAT CTACCCGATG ATCTCGACTA CCCCAAGGAA 
CAGCTGACAG AATCATCTAT TAAGGCTTTA ACAGAACAAA ACAGAGAACT AATGGAAACC 
GGCCGGAGCG TTGTTCAAAA ATATTTGAAC GATTGTGGAC GTCGCTACGT TGATATGACT 
AGCATATTTG ATTTCATGCT AAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_014297.3
CDS	1..4407
Translation	MSSKSEKLEKLRKLQAARNGTSIDDYEGDESDGDRIYDEIDEKEYRARKRQELLHDDFVVDDDGVGYVDRGVEEDWREVDNSSSDEDTGNLASKDSKRKKNIKREKDHQITDMLRTQHSKSTLLAHAKKSQKKSIPIDNFDDILGEFESGEVEKPNILLPSKLRENLNSSPTSEFKSSIKRVNGNDESSHDAGISKKVKIDPDSSTDKYLEIESSPLKLQSRKLRYANDVQDLLDDVENSPVVATKRQNVLQDTLLANPPSAQSLADEEDDEDSDEDIILKRRTMRSVTTTRRVNIDSRSNPSTSPFVTAPGTPIGIKGLTPSKSLQSNTDVATLAVNVKKEDVVDPETDTFQMFWLDYCEVNNTLILFGKVKLKDDNCVSAMVQINGLCRELFFLPREGKTPTDIHEEIIPLLMDKYGLDNIRAKPQKMKYSFELPDIPSESDYLKVLLPYQTPKSSRDTIPSDLSSDTFYHVFGGNSNIFESFVIQNRIMGPCWLDIKGADFNSIRNASHCAVEVSVDKPQNITPTTTKTMPNLRCLSLSIQTLMNPKENKQEIVSITLSAYRNISLDSPIPENIKPDDLCTLVRPPQSTSFPLGLAALAKQKLPGRVRLFNNEKAMLSCFCAMLKVEDPDVIIGHRLQNVYLDVLAHRMHDLNIPTFSSIGRRLRRTWPEKFGRGNSNMNHFFISDICSGRLICDIANEMGQSLTPKCQSWDLSEMYQVTCEKEHKPLDIDYQNPQYQNDVNSMTMALQENITNCMISAEVSYRIQLLTLTKQLTNLAGNAWAQTLGGTRAGRNEYILLHEFSRNGFIVPDKEGNRSRAQKQRQNEENADAPVNSKKAKYQGGLVFEPEKGLHKNYVLVMDFNSLYPSIIQEFNICFTTVDRNKEDIDELPSVPPSEVDQGVLPRLLANLVDRRREVKKVMKTETDPHKRVQCDIRQQALKLTANSMYGCLGYVNSRFYAKPLAMLVTNKGREILMNTRQLAESMNLLVVYGDTDSVMIDTGCDNYADAIKIGLGFKRLVNERYRLLEIDIDNVFKKLLLHAKKKYAALTVNLDKNGNGTTVLEVKGLDMKRREFCPLSRDVSIHVLNTILSDKDPEEALQEVYDYLEDIRIKVETNNIRIDKYKINMKLSKDPKAYPGGKNMPAVQVALRMRKAGRVVKAGSVITFVITKQDEIDNAADTPALSVAERAHALNEVMIKSNNLIPDPQYYLEKQIFAPVERLLERIDSFNVVRLSEALGLDSKKYFRREGGNNNGEDINNLQPLETTITDVERFKDTVTLELSCPSCDKRFPFGGIVSSNYYRVSYNGLQCKHCEQLFTPLQLTSQIEHSIRAHISLYYAGWLQCDDSTCGIVTRQVSVFGKRCLNDGCTGVMRYKYSDKQLYNQLLYFDSLFDCEKNKKQELKPIYLPDDLDYPKEQLTESSIKALTEQNRELMETGRSVVQKYLNDCGRRYVDMTSIFDFMLN

Target ORF information:

RefSeq Version	NM_001182940.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C DNA-directed DNA polymerase alpha catalytic subunit POL1 (POL1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001182940.3

ORF Insert Sequence:

ATGTCTTCTA AAAGTGAAAA ATTAGAAAAA TTGAGAAAGC TGCAAGCCGC TCGAAATGGT 
ACATCCATCG ATGATTACGA AGGTGACGAA TCAGATGGTG ACAGAATATA CGACGAAATC 
GACGAAAAAG AATACAGGGC CAGAAAGCGC CAGGAATTGC TGCATGACGA TTTTGTTGTA 
GATGACGATG GTGTAGGCTA CGTCGATCGT GGTGTCGAAG AAGATTGGAG GGAAGTTGAT 
AACAGTTCAA GTGATGAAGA CACTGGAAAC CTTGCTAGCA AGGATTCTAA AAGGAAGAAG 
AATATAAAAA GGGAGAAAGA CCACCAGATT ACAGATATGC TGCGAACTCA ACATTCCAAA 
TCAACACTGC TAGCACATGC AAAAAAATCC CAAAAAAAAA GTATCCCCAT CGATAATTTT 
GATGACATTC TTGGTGAGTT TGAATCTGGT GAAGTAGAAA AACCCAATAT TTTACTGCCA 
TCCAAATTAA GGGAAAATTT AAATTCTTCG CCGACTAGTG AATTTAAATC GTCAATAAAG 
AGAGTTAACG GAAACGATGA GTCAAGTCAT GATGCCGGTA TTTCTAAAAA AGTCAAGATT 
GATCCAGATT CAAGTACTGA TAAATATTTA GAAATCGAAT CATCACCGTT AAAATTACAA 
AGCAGGAAAC TGCGCTACGC AAATGATGTA CAGGATTTAT TGGATGATGT GGAAAATTCC 
CCAGTAGTAG CCACAAAGAG ACAGAATGTA CTTCAAGATA CGTTACTAGC AAATCCACCA 
TCCGCTCAAA GTTTGGCTGA TGAAGAAGAT GATGAAGATA GTGATGAAGA TATTATTCTG 
AAAAGAAGAA CTATGAGAAG TGTGACAACA ACTAGACGCG TGAATATAGA CTCAAGAAGT 
AACCCTTCAA CTTCACCGTT TGTCACAGCA CCCGGAACGC CAATCGGTAT TAAAGGACTA 
ACTCCAAGTA AATCTTTGCA GAGCAACACC GATGTTGCTA CACTTGCGGT TAATGTCAAA 
AAGGAAGATG TGGTAGATCC GGAAACTGAT ACTTTCCAAA TGTTTTGGTT GGATTACTGT 
GAAGTGAACA ACACTTTGAT TTTATTTGGT AAGGTTAAAC TAAAGGATGA TAATTGTGTG 
TCAGCCATGG TTCAAATCAA CGGTCTCTGT AGAGAGCTAT TTTTCCTTCC AAGAGAGGGT 
AAAACACCTA CCGATATACA CGAGGAAATA ATTCCGTTAC TGATGGATAA ATATGGACTG 
GATAATATCC GCGCTAAACC TCAAAAAATG AAATATTCGT TTGAGCTTCC TGACATTCCA 
TCTGAAAGTG ATTATTTGAA AGTCTTACTT CCATACCAAA CACCGAAGTC CAGTCGAGAC 
ACCATTCCAT CGGATTTATC TAGCGATACC TTTTATCATG TTTTTGGTGG GAATTCCAAT 
ATTTTTGAAA GTTTTGTTAT TCAAAATAGG ATTATGGGAC CATGCTGGTT AGATATAAAG 
GGAGCAGATT TCAATTCAAT TCGTAATGCT TCTCACTGTG CGGTTGAAGT TTCGGTAGAT 
AAGCCTCAGA ATATCACGCC TACTACAACA AAGACAATGC CTAATCTGAG ATGTCTATCT 
TTATCGATTC AGACGTTAAT GAATCCCAAG GAAAATAAGC AAGAGATTGT TTCGATAACG 
TTATCAGCAT ATAGAAATAT CTCGTTGGAT TCACCGATAC CTGAAAATAT AAAACCAGAC 
GACTTGTGTA CTTTAGTAAG GCCGCCTCAG TCAACTAGTT TCCCATTAGG TTTAGCTGCA 
CTGGCAAAAC AGAAACTACC CGGTAGAGTC AGGTTGTTCA ACAACGAAAA GGCCATGCTA 
TCTTGTTTTT GTGCTATGTT AAAAGTGGAA GACCCAGATG TTATCATAGG CCATCGTTTG 
CAAAATGTCT ACTTAGATGT TTTAGCTCAT AGAATGCATG ATCTCAACAT TCCAACGTTC 
AGCTCTATTG GCCGTCGTTT AAGAAGGACT TGGCCTGAAA AATTCGGTAG AGGAAATTCG 
AATATGAACC ATTTTTTTAT TAGTGACATT TGCTCTGGTA GGCTGATATG TGATATCGCC 
AATGAAATGG GTCAATCATT AACACCCAAA TGTCAAAGTT GGGATCTTTC AGAGATGTAT 
CAAGTTACAT GTGAAAAGGA GCATAAGCCG TTAGATATTG ATTATCAAAA TCCACAATAC 
CAAAATGATG TAAATAGTAT GACAATGGCC CTACAAGAGA ATATTACTAA TTGTATGATT 
TCTGCTGAGG TGTCTTATAG AATTCAATTA TTAACCTTGA CTAAACAGTT GACAAATTTG 
GCTGGTAATG CATGGGCCCA AACGCTAGGC GGTACAAGAG CTGGTAGAAA TGAGTATATC 
TTACTACATG AATTTTCAAG AAATGGTTTT ATTGTTCCTG ACAAAGAAGG CAATAGAAGT 
AGAGCTCAGA AACAAAGACA AAATGAAGAA AATGCTGATG CACCAGTTAA TTCTAAAAAG 
GCAAAATATC AGGGTGGTTT AGTTTTTGAA CCTGAGAAAG GTCTTCATAA GAATTACGTT 
TTAGTCATGG ACTTTAATTC TTTGTATCCA TCTATTATCC AGGAATTTAA TATATGTTTT 
ACCACCGTTG ATAGAAATAA GGAAGATATC GATGAGTTAC CAAGCGTACC TCCTTCAGAG 
GTGGATCAGG GTGTTTTACC AAGGTTATTA GCTAATCTGG TGGATCGTCG ACGTGAAGTT 
AAGAAGGTGA TGAAAACTGA AACTGATCCC CATAAGCGAG TTCAATGTGA TATTCGTCAA 
CAAGCTCTAA AATTGACTGC CAATTCTATG TATGGTTGTT TGGGTTATGT TAACAGTAGA 
TTTTACGCAA AGCCGCTCGC CATGTTAGTC ACTAATAAGG GTCGTGAGAT TTTAATGAAT 
ACAAGACAAC TAGCTGAAAG TATGAATCTT CTTGTAGTTT ATGGTGATAC AGATTCGGTC 
ATGATAGATA CCGGTTGTGA TAATTATGCG GATGCAATTA AAATTGGCTT GGGATTTAAA 
AGGCTAGTAA ATGAGCGCTA TAGATTATTG GAGATTGATA TTGATAATGT TTTTAAGAAG 
TTACTATTAC ATGCCAAGAA AAAGTACGCT GCTTTGACTG TAAATTTGGA CAAAAATGGT 
AATGGAACTA CTGTTCTAGA AGTTAAAGGG TTGGACATGA AGCGTCGTGA ATTTTGTCCA 
CTTTCGAGGG ACGTTTCTAT ACATGTTTTA AACACCATCC TGTCAGATAA GGACCCAGAA 
GAAGCATTGC AAGAAGTGTA TGATTACTTA GAAGACATCA GAATAAAAGT GGAAACCAAT 
AACATTAGAA TTGATAAATA TAAGATCAAT ATGAAGCTTT CAAAAGATCC CAAGGCCTAC 
CCAGGTGGTA AAAACATGCC TGCAGTCCAA GTAGCTCTAA GAATGCGTAA GGCTGGTAGA 
GTTGTTAAAG CTGGCTCTGT CATCACTTTT GTGATCACAA AGCAGGATGA AATAGACAAT 
GCAGCGGATA CGCCGGCTCT TTCTGTGGCT GAACGTGCCC ATGCATTGAA TGAGGTAATG 
ATTAAAAGTA ACAATTTGAT ACCTGATCCA CAATATTATC TCGAGAAACA AATATTCGCG 
CCGGTAGAAA GGTTGTTAGA AAGAATTGAT AGCTTCAACG TGGTGCGTTT GAGTGAAGCG 
CTTGGTTTAG ATAGTAAAAA GTATTTTAGA AGAGAGGGCG GTAATAACAA TGGAGAAGAT 
ATTAATAATT TGCAACCCTT AGAAACAACA ATTACAGACG TTGAAAGGTT TAAGGATACT 
GTAACATTAG AATTAAGTTG CCCATCATGC GATAAAAGGT TTCCATTTGG TGGTATTGTA 
TCTTCAAATT ACTATCGCGT GTCATATAAT GGTTTACAGT GCAAGCATTG TGAGCAACTT 
TTTACTCCTC TTCAATTAAC TAGCCAAATA GAGCATTCTA TAAGGGCACA CATTTCCTTA 
TATTACGCAG GGTGGTTACA GTGTGATGAC AGCACATGCG GTATAGTTAC AAGACAAGTC 
TCCGTTTTTG GTAAGCGTTG TTTGAATGAC GGCTGCACGG GTGTCATGAG ATACAAATAC 
AGTGACAAGC AATTGTACAA TCAACTTTTA TATTTCGATT CTTTATTCGA TTGTGAAAAA 
AATAAAAAGC AAGAATTGAA GCCAATATAT CTACCCGATG ATCTCGACTA CCCCAAGGAA 
CAGCTGACAG AATCATCTAT TAAGGCTTTA ACAGAACAAA ACAGAGAACT AATGGAAACC 
GGCCGGAGCG TTGTTCAAAA ATATTTGAAC GATTGTGGAC GTCGCTACGT TGATATGACT 
AGCATATTTG ATTTCATGCT AAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.
Nature387(6632 Suppl)93-8(1997 May)
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POL1 cDNA ORF clone, Saccharomyces cerevisiae S288C

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ORF Insert Sequence:

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