ICU2 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	ICU2
Entrez Gene ID	836845
Full Name	DNA-directed DNA polymerase
Synonyms	INCURVATA2,K21H1.14,K21H1_14
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_126110.3	NP_201511.2	DNA-directed DNA polymerase
NM_001345811.1	NP_001331725.1	DNA-directed DNA polymerase

Pathways from BioSystems

Homologies

	POLA1	XP_001091195.1
Gallus gallus (chicken)	POLA1	XP_416792.3
Eremothecium gossypii	AGOS_AER381C	NP_985237.1
Homo sapiens (human)	POLA1	NP_058633.2
Rattus norvegicus (Norway rat)	Pola1	NP_445931.1
Schizosaccharomyces pombe (fission yeast)	pol1	NP_594186.1
Pan troglodytes (chimpanzee)	POLA1	XP_003317445.2
Mus musculus (house mouse)	Pola1	NP_032918.1
Danio rerio (zebrafish)	pola1	XP_005162809.1
Drosophila melanogaster (fruit fly)	DNApol-alpha180	NP_536736.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001929	XP_321134.5
Caenorhabditis elegans (roundworm)	Y47D3A.29	NP_001255109.1
Magnaporthe oryzae (rice blast fungus)	MGG_06397	XP_003717174.1
Neurospora crassa	NCU07870	XP_963131.2
Arabidopsis thaliana (thale cress)	ICU2	NP_201511.2
Xenopus tropicalis (tropical clawed frog)	pola1	XP_004911812.1
Bos taurus (cattle)	POLA1	NP_001192994.1
Saccharomyces cerevisiae (baker's yeast)	POL1	NP_014297.3
Kluyveromyces lactis	KLLA0C13255g	XP_452791.1
Canis lupus familiaris (dog)	POLA1	XP_005641271.1
Oryza sativa (rice)	Os01g0868300	NP_001044920.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T, Asamizu E, Miyajima N, Sasamoto S, Kimura T, Hosouchi T, Kawashima K, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Naruo K, Okumura S, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Sato S, de la Bastide M, Huang E, Spiegel L, Gnoj L, O'Shaughnessy A, Preston R, Habermann K, Murray J, Johnson D, Rohlfing T, Nelson J, Stoneking T, Pepin K, Spieth J, Sekhon M, Armstrong J, Becker M, Belter E, Cordum H, Cordes M, Courtney L, Courtney W, Dante M, Du H, Edwards J, Fryman J, Haakensen B, Lamar E, Latreille P, Leonard S, Meyer R, Mulvaney E, Ozersky P, Riley A, Strowmatt C, Wagner-McPherson C, Wollam A, Yoakum M, Bell M, Dedhia N, Parnell L, Shah R, Rodriguez M, See LH, Vil D, Baker J, Kirchoff K, Toth K, King L, Bahret A, Miller B, Marra M, Martienssen R, McCombie WR, Wilson RK, Murphy G, Bancroft I, Volckaert G, Wambutt R, D?sterh?ft A, Stiekema W, Pohl T, Entian KD, Terryn N, Hartley N, Bent E, Johnson S, Langham SA, McCullagh B, Robben J, Grymonprez B, Zimmermann W, Ramsperger U, Wedler H, Balke K, Wedler E, Peters S, van Staveren M, Dirkse W, Mooijman P, Lankhorst RK, Weitzenegger T, Bothe G, Rose M, Hauf J, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Villarroel R, Gielen J, Ardiles W, Bents O, Lemcke K, Kolesov G, Mayer K, Rudd S, Schoof H, Schueller C, Zaccaria P, Mewes HW, Bevan M, Fransz P, , ,
Nature408(6814)823-6(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results uncover a role for ICU2 in the regulation of genes involved in abscisic acid signaling as well as in salicylic acid biosynthesis and accumulation.
Title: Arabidopsis INCURVATA2 Regulates Salicylic Acid and Abscisic Acid Signaling, and Oxidative Stress Responses.

Dna polymerase alpha plays a genome-wide role in DNA replication and telomere stability.
Title: Role of STN1 and DNA polymerase ? in telomere stability and genome-wide replication in Arabidopsis.

ICU2 facilitates histone assembly in dividing cells, demonstrating a possible mechanism for ICU2-mediated epigenetic maintenance.
Title: The catalytic subunit of Arabidopsis DNA polymerase ? ensures stable maintenance of histone modification.

Results suggest that DNA polymerase alpha is involved in mediating epigenetic states and in DNA homologous recombination in Arabidopsis.
Title: Mutation in the catalytic subunit of DNA polymerase alpha influences transcriptional gene silencing and homologous recombination in Arabidopsis.

The positional cloning and characterization of INCURVATA2 (ICU2), which encodes the putative catalytic subunit of the DNA polymerase alpha of Arabidopsis thaliana.
Title: INCURVATA2 encodes the catalytic subunit of DNA Polymerase alpha and interacts with genes involved in chromatin-mediated cellular memory in Arabidopsis thaliana.

The following ICU2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ICU2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb22679	NM_126110.3 Latest version!	Arabidopsis thaliana DNA-directed DNA polymerase (ICU2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OAb22679	NM_001345811.1 Latest version!	Arabidopsis thaliana DNA-directed DNA polymerase (ICU2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ICU2 ( NM_126110.3 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_201511.2 Arabidopsis thaliana DNA-directed DNA polymerase (ICU2), partial mRNA.

Price & Availability↑

CloneID	OAb22679
Clone ID Related Accession (Same CDS sequence)	NM_126110.3 , NM_001345811.1
Accession Version	NM_126110.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4575bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	DNA-directed DNA polymerase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Sep 12, 2016 this sequence version replaced NM_126110.2. COMPLETENESS: incomplete on the 3' end.

ATGTCCGGCG ATAATTCAAC GGAAACTGGA CGAAGACGTA GCCGAGGCGC GGAGGCGTCG 
AGTAGGAAAG ACACACTCGA GCGTCTGAAG GCGATTCGAC AAGGAGGAAT CCGTTCTGCG 
TCAGGTGGTG GGTACGATAT TAGGCTGCAG AAGCCAATCT TCGATACCGT TGATGACGAA 
GAGTACGACG CACTTGTTTC CCGGCGGCGC GAGGAGGCTC GCGGGTTTGT AGTTGAAGAT 
GGGGAAGGTG GCGATTTGGG ATATCTCGAC GAAGGTGAGG AGGAGGATTG GTCGAAGCCA 
AGTGGACCAG AATCGACTGA TGAATCAGAC GACGGTGGTA GGTTTAGTGG GAGACTTAAG 
AAGAAGAAGA AAGGGAAAGA GCAAACGCAG CAACCTCAGG TTAAGAAGGT GAATCCGGCG 
TTAAAAGCTG CGGCTACTAT CACTGGTGAA GGTAGACTTT CGTCTATGTT CACATCTTCG 
AGTTTCAAGA AAGTTAAGGA GACTGATAAG GCACAGTATG AGGGTATTCT TGACGAGATA 
ATAGCTCAGG TTACTCCAGA TGAGTCGGAT AGAAAAAAGC ATACGAGGAG AAAACTACCA 
GGTACTGTTC CAGTTACCAT CTTCAAGAAT AAAAAACTGT TTTCTGTTGC CTCGAGTATG 
GGAATGAAAG AGTCTGAACC AACACCTTCA ACTTATGAGG GTGATTCTGT TTCCATGGAT 
AATGAATTGA TGAAAGAGGA AGATATGAAA GAATCTGAAG TCATTCCTTC TGAGACTATG 
GAATTACTTG GAAGTGATAT TGTCAAAGAG GATGGATCAA ACAAGATAAG GAAAACTGAA 
GTGAAGAGTG AGTTAGGTGT CAAGGAGGTA TTTACATTGA ATGCAACGAT TGATATGAAA 
GAGAAGGATT CGGCTTTAAG TGCCACTGCT GGTTGGAAGG AAGCGATGGG AAAAGTTGGA 
ACTGAGAATG GGGCTTTGCT TGGTTCTAGT TCTGAAGGGA AAACGGAATT TGATTTGGAT 
GCTGACGGAT CACTTCGTTT CTTCATTCTT GACGCTTATG AGGAGGCTTT TGGTGCGAGC 
ATGGGCACAA TATATCTGTT TGGGAAGGTT AAAATGGGAG ATACTTACAA GAGTTGCTGT 
GTGGTAGTTA AGAATATCCA GAGGTGTGTT TATGCTATTC CAAATGATTC CATATTTCCT 
TCCCATGAAC TAATCATGCT CGAGCAAGAG GTGAAAGACT CTCGACTTTC TCCTGAATCC 
TTCCGTGGGA AATTGCATGA AATGGCATCA AAACTGAAAA ACGAAATTGC TCAGGAATTG 
TTACAGCTCA ATGTTTCAAA TTTTAGCATG GCGCCTGTCA AGAGAAACTA TGCTTTTGAG 
AGGCCTGATG TGCCTGCTGG CGAACAATAT GTTTTGAAGA TAAATTATTC ATTTAAGGAT 
CGTCCACTTC CAGAAGATCT TAAAGGCGAG TCTTTCTCCG CTTTGCTCGG GTCTCATACC 
AGTGCTTTGG AGCATTTTAT CCTTAAGAGG AAGATCATGG GACCTTGTTG GCTCAAAATT 
TCAAGCTTCT CTACTTGCTC TCCTTCGGAA GGAGTGAGTT GGTGCAAGTT TGAGGTTACT 
GTTCAATCTC CCAAGGATAT CACTATTTTA GTTTCAGAGG AAAAGGTGGT TCACCCTCCT 
GCTGTTGTTA CAGCCATAAA TTTAAAGACT ATAGTCAATG AAAAGCAGAA CATAAGCGAA 
ATTGTTTCTG CATCTGTTCT CTGCTTTCAC AACGCCAAGA TTGATGTTCC AATGCCAGCT 
CCAGAAAGAA AGAGATCTGG TATTCTGAGT CATTTCACTG TTGTTAGGAA TCCTGAAGGA 
ACCGGTTACC CAATTGGTTG GAAAAAAGAA GTGTCTGACA GAAATTCAAA GAACGGCTGC 
AATGTATTAA GTATTGAAAA CAGTGAAAGA GCATTGCTGA ACCGATTGTT TTTGGAGTTA 
AACAAATTGG ACAGTGATAT TCTTGTGGGA CATAATATTT CAGGTTTTGA TCTGGATGTT 
CTTCTTCAAA GGGCCCAGGC TTGCAAAGTT CAGAGTAGCA TGTGGTCCAA AATTGGCCGA 
CTCAAACGCT CTTTTATGCC TAAGCTTAAA GGAAACAGTA ATTATGGGTC AGGAGCTACA 
CCAGGGCTCA TGTCTTGTAT TGCTGGAAGG CTCTTGTGTG ATACAGATCT GTGTTCTCGT 
GACTTGTTGA AGGAGGTCAG TTATTCTTTA ACGGACCTCT CAAAGACACA GCTGAACCGG 
GACAGGAAAG AGATAGCGCC TAATGATATT CCCAAAATGT TTCAGTCATC CAAAACACTT 
GTGGAACTTA TTGAATGTGG TGAGACGGAT GCATGGTTAT CCATGGAGCT CATGTTTCAT 
CTAAGTGTTC TTCCTCTCAC TCTCCAGCTT ACTAACATAA GTGGCAATCT TTGGGGGAAA 
ACACTTCAAG GAGCCAGGGC ACAGAGAATC GAATACTACT TACTACATAC ATTCCACTCG 
AAAAAGTTCA TTCTCCCTGA CAAAATTTCA CAACGTATGA AGGAAATAAA GTCATCAAAA 
AGAAGAATGG ATTATGCTCC AGAAGACCGC AATGTAGATG AATTGGATGC TGACCTGACT 
TTGGAGAATG ATCCGTCTAA GGGCAGCAAA ACAAAAAAAG GTCCAGCCTA CGCTGGTGGA 
CTGGTCTTGG AGCCAAAGAG AGGCTTATAT GACAAATATG TCTTGCTTCT GGATTTCAAT 
AGTCTTTACC CTTCTATTAT ACAGGAATAC AATATCTGTT TTACGACTAT ACCACGATCA 
GAAGATGGAG TTCCTCGTTT ACCTTCGAGT CAGACACCTG GAATTCTTCC AAAGTTGATG 
GAACATTTGG TCAGTATAAG GAAAAGCGTC AAATTAAAGA TGAAGAAGGA AACAGGTCTC 
AAGTACTGGG AGCTTGACAT TCGACAGCAG GCATTGAAGC TCACAGCCAA TAGTATGTAT 
GGCTGTCTGG GGTTTTCTAA TTCAAGATTC TATGCTAAGC CTTTAGCAGA GCTCATAACA 
CTACAGGGAA GGGATATCCT GCAAAGAACC GTGGATCTTG TCCAGAACCA TTTAAATCTA 
GAGGTAATAT ACGGTGACAC AGATTCAATC ATGATTCACA GCGGACTAGA TGATATTGAA 
GAGGTGAAAG CCATTAAATC AAAAGTCATC CAAGAGGTCA ATAAGAAGTA TAGATGTCTA 
AAAATTGACT GTGATGGCAT ATACAAGAGA ATGCTTCTTC TTCGAAAAAA GAAGTATGCT 
GCTGTCAAGT TGCAGTTTAA GGATGGGAAG CCTTGCGAGG ACATTGAAAG AAAAGGTGTG 
GATATGGTTC GTCGAGATTG GAGCTTACTC TCGAAGGAAA TAGGAGATTT ATGCCTGAGT 
AAAATCTTGT ATGGAGGATC CTGTGAGGAT GTTGTTGAAG CAATTCATAA CGAACTAATG 
AAGATAAAAG AGGAAATGAG AAATGGGCAA GTTGCTCTTG AGAAGTATGT CATCACCAAG 
ACATTGACTA AACCGCCTGC AGCTTATCCA GATTCCAAAA GCCAACCACA CGTTCAAGTT 
GCACTCAGAA TGAGGCAACG TGGTTATAAA GAAGGTTTCA ATGCTAAGGA TACTGTACCG 
TATATAATCT GCTATGAGCA GGGTAATGCT AGCTCTGCTA GTTCAGCAGG AATTGCCGAA 
CGTGCTAGGC ATCCTGATGA GGTGAAATCA GAAGGCAGCA GATGGTTGGT TGACATAGAT 
TACTACTTAG CACAACAGAT TCATCCGGTG GTGTCTCGTC TATGCGCAGA GATTCAAGGA 
ACAAGTCCTG AACGTCTAGC TGAATGTTTA GGACTTGACC CATCAAAGTA CCGAAGCAAA 
TCAAACGATG CTACAAGCAG TGATCCCTCT ACCTCCCTCT TATTCGCAAC AAGCGATGAA 
GAAAGATATA AAAGTTGCGA GCCTTTAGCA TTAACATGCC CCAGCTGCTC TACTGCTTTC 
AACTGTCCAT CTATTATTAG TTCTGTTTGT GCGTCAATCA GTAAGAAACC TGCAACACCT 
GAAACAGAGG AATCTGATTC CACATTCTGG CTTAAACTGC ATTGCCCGAA ATGTCAGCAG 
GAAGATAGCA CGGGAATAAT ATCCCCTGCA ATGATAGCTA ACCAGGTAAA AAGACAGATC 
GATGGATTTG TGTCAATGTA CTACAAAGGC ATAATGGTGT GTGAAGACGA GTCATGCAAG 
CACACAACCC GCTCCCCCAA CTTTCGTCTA CTCGGTGAAC GTGAACGGGG GACAGTTTGC 
CCAAACTATC CTAACTGTAA CGGAACCCTC TTACGAAAGT ACACTGAAGC AGACTTGTAC 
AAGCAACTGT CATACTTCTG CCACATCTTA GACACACAAT GCTCTTTAGA AAAGATGGAT 
GTTGGCGTGA GAATTCAAGT AGAGAAAGCA ATGACGAAGA TAAGACCTGC GGTTAAGTCA 
GCAGCAGCAA TCACTCGAAG TAGTAGAGAC CGGTGTGCTT ATGGATGGAT GCAACTCACA 
GACATAGTCA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_201511.2
CDS	24..4598
Translation	MSGDNSTETGRRRSRGAEASSRKDTLERLKAIRQGGIRSASGGGYDIRLQKPIFDTVDDEEYDALVSRRREEARGFVVEDGEGGDLGYLDEGEEEDWSKPSGPESTDESDDGGRFSGRLKKKKKGKEQTQQPQVKKVNPALKAAATITGEGRLSSMFTSSSFKKVKETDKAQYEGILDEIIAQVTPDESDRKKHTRRKLPGTVPVTIFKNKKLFSVASSMGMKESEPTPSTYEGDSVSMDNELMKEEDMKESEVIPSETMELLGSDIVKEDGSNKIRKTEVKSELGVKEVFTLNATIDMKEKDSALSATAGWKEAMGKVGTENGALLGSSSEGKTEFDLDADGSLRFFILDAYEEAFGASMGTIYLFGKVKMGDTYKSCCVVVKNIQRCVYAIPNDSIFPSHELIMLEQEVKDSRLSPESFRGKLHEMASKLKNEIAQELLQLNVSNFSMAPVKRNYAFERPDVPAGEQYVLKINYSFKDRPLPEDLKGESFSALLGSHTSALEHFILKRKIMGPCWLKISSFSTCSPSEGVSWCKFEVTVQSPKDITILVSEEKVVHPPAVVTAINLKTIVNEKQNISEIVSASVLCFHNAKIDVPMPAPERKRSGILSHFTVVRNPEGTGYPIGWKKEVSDRNSKNGCNVLSIENSERALLNRLFLELNKLDSDILVGHNISGFDLDVLLQRAQACKVQSSMWSKIGRLKRSFMPKLKGNSNYGSGATPGLMSCIAGRLLCDTDLCSRDLLKEVSYSLTDLSKTQLNRDRKEIAPNDIPKMFQSSKTLVELIECGETDAWLSMELMFHLSVLPLTLQLTNISGNLWGKTLQGARAQRIEYYLLHTFHSKKFILPDKISQRMKEIKSSKRRMDYAPEDRNVDELDADLTLENDPSKGSKTKKGPAYAGGLVLEPKRGLYDKYVLLLDFNSLYPSIIQEYNICFTTIPRSEDGVPRLPSSQTPGILPKLMEHLVSIRKSVKLKMKKETGLKYWELDIRQQALKLTANSMYGCLGFSNSRFYAKPLAELITLQGRDILQRTVDLVQNHLNLEVIYGDTDSIMIHSGLDDIEEVKAIKSKVIQEVNKKYRCLKIDCDGIYKRMLLLRKKKYAAVKLQFKDGKPCEDIERKGVDMVRRDWSLLSKEIGDLCLSKILYGGSCEDVVEAIHNELMKIKEEMRNGQVALEKYVITKTLTKPPAAYPDSKSQPHVQVALRMRQRGYKEGFNAKDTVPYIICYEQGNASSASSAGIAERARHPDEVKSEGSRWLVDIDYYLAQQIHPVVSRLCAEIQGTSPERLAECLGLDPSKYRSKSNDATSSDPSTSLLFATSDEERYKSCEPLALTCPSCSTAFNCPSIISSVCASISKKPATPETEESDSTFWLKLHCPKCQQEDSTGIISPAMIANQVKRQIDGFVSMYYKGIMVCEDESCKHTTRSPNFRLLGERERGTVCPNYPNCNGTLLRKYTEADLYKQLSYFCHILDTQCSLEKMDVGVRIQVEKAMTKIRPAVKSAAAITRSSRDRCAYGWMQLTDIVI

Target ORF information:

RefSeq Version	NM_126110.3
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana DNA-directed DNA polymerase (ICU2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_126110.3

ORF Insert Sequence:

ATGTCCGGCG ATAATTCAAC GGAAACTGGA CGAAGACGTA GCCGAGGCGC GGAGGCGTCG 
AGTAGGAAAG ACACACTCGA GCGTCTGAAG GCGATTCGAC AAGGAGGAAT CCGTTCTGCG 
TCAGGTGGTG GGTACGATAT TAGGCTGCAG AAGCCAATCT TCGATACCGT TGATGACGAA 
GAGTACGACG CACTTGTTTC CCGGCGGCGC GAGGAGGCTC GCGGGTTTGT AGTTGAAGAT 
GGGGAAGGTG GCGATTTGGG ATATCTCGAC GAAGGTGAGG AGGAGGATTG GTCGAAGCCA 
AGTGGACCAG AATCGACTGA TGAATCAGAC GACGGTGGTA GGTTTAGTGG GAGACTTAAG 
AAGAAGAAGA AAGGGAAAGA GCAAACGCAG CAACCTCAGG TTAAGAAGGT GAATCCGGCG 
TTAAAAGCTG CGGCTACTAT CACTGGTGAA GGTAGACTTT CGTCTATGTT CACATCTTCG 
AGTTTCAAGA AAGTTAAGGA GACTGATAAG GCACAGTATG AGGGTATTCT TGACGAGATA 
ATAGCTCAGG TTACTCCAGA TGAGTCGGAT AGAAAAAAGC ATACGAGGAG AAAACTACCA 
GGTACTGTTC CAGTTACCAT CTTCAAGAAT AAAAAACTGT TTTCTGTTGC CTCGAGTATG 
GGAATGAAAG AGTCTGAACC AACACCTTCA ACTTATGAGG GTGATTCTGT TTCCATGGAT 
AATGAATTGA TGAAAGAGGA AGATATGAAA GAATCTGAAG TCATTCCTTC TGAGACTATG 
GAATTACTTG GAAGTGATAT TGTCAAAGAG GATGGATCAA ACAAGATAAG GAAAACTGAA 
GTGAAGAGTG AGTTAGGTGT CAAGGAGGTA TTTACATTGA ATGCAACGAT TGATATGAAA 
GAGAAGGATT CGGCTTTAAG TGCCACTGCT GGTTGGAAGG AAGCGATGGG AAAAGTTGGA 
ACTGAGAATG GGGCTTTGCT TGGTTCTAGT TCTGAAGGGA AAACGGAATT TGATTTGGAT 
GCTGACGGAT CACTTCGTTT CTTCATTCTT GACGCTTATG AGGAGGCTTT TGGTGCGAGC 
ATGGGCACAA TATATCTGTT TGGGAAGGTT AAAATGGGAG ATACTTACAA GAGTTGCTGT 
GTGGTAGTTA AGAATATCCA GAGGTGTGTT TATGCTATTC CAAATGATTC CATATTTCCT 
TCCCATGAAC TAATCATGCT CGAGCAAGAG GTGAAAGACT CTCGACTTTC TCCTGAATCC 
TTCCGTGGGA AATTGCATGA AATGGCATCA AAACTGAAAA ACGAAATTGC TCAGGAATTG 
TTACAGCTCA ATGTTTCAAA TTTTAGCATG GCGCCTGTCA AGAGAAACTA TGCTTTTGAG 
AGGCCTGATG TGCCTGCTGG CGAACAATAT GTTTTGAAGA TAAATTATTC ATTTAAGGAT 
CGTCCACTTC CAGAAGATCT TAAAGGCGAG TCTTTCTCCG CTTTGCTCGG GTCTCATACC 
AGTGCTTTGG AGCATTTTAT CCTTAAGAGG AAGATCATGG GACCTTGTTG GCTCAAAATT 
TCAAGCTTCT CTACTTGCTC TCCTTCGGAA GGAGTGAGTT GGTGCAAGTT TGAGGTTACT 
GTTCAATCTC CCAAGGATAT CACTATTTTA GTTTCAGAGG AAAAGGTGGT TCACCCTCCT 
GCTGTTGTTA CAGCCATAAA TTTAAAGACT ATAGTCAATG AAAAGCAGAA CATAAGCGAA 
ATTGTTTCTG CATCTGTTCT CTGCTTTCAC AACGCCAAGA TTGATGTTCC AATGCCAGCT 
CCAGAAAGAA AGAGATCTGG TATTCTGAGT CATTTCACTG TTGTTAGGAA TCCTGAAGGA 
ACCGGTTACC CAATTGGTTG GAAAAAAGAA GTGTCTGACA GAAATTCAAA GAACGGCTGC 
AATGTATTAA GTATTGAAAA CAGTGAAAGA GCATTGCTGA ACCGATTGTT TTTGGAGTTA 
AACAAATTGG ACAGTGATAT TCTTGTGGGA CATAATATTT CAGGTTTTGA TCTGGATGTT 
CTTCTTCAAA GGGCCCAGGC TTGCAAAGTT CAGAGTAGCA TGTGGTCCAA AATTGGCCGA 
CTCAAACGCT CTTTTATGCC TAAGCTTAAA GGAAACAGTA ATTATGGGTC AGGAGCTACA 
CCAGGGCTCA TGTCTTGTAT TGCTGGAAGG CTCTTGTGTG ATACAGATCT GTGTTCTCGT 
GACTTGTTGA AGGAGGTCAG TTATTCTTTA ACGGACCTCT CAAAGACACA GCTGAACCGG 
GACAGGAAAG AGATAGCGCC TAATGATATT CCCAAAATGT TTCAGTCATC CAAAACACTT 
GTGGAACTTA TTGAATGTGG TGAGACGGAT GCATGGTTAT CCATGGAGCT CATGTTTCAT 
CTAAGTGTTC TTCCTCTCAC TCTCCAGCTT ACTAACATAA GTGGCAATCT TTGGGGGAAA 
ACACTTCAAG GAGCCAGGGC ACAGAGAATC GAATACTACT TACTACATAC ATTCCACTCG 
AAAAAGTTCA TTCTCCCTGA CAAAATTTCA CAACGTATGA AGGAAATAAA GTCATCAAAA 
AGAAGAATGG ATTATGCTCC AGAAGACCGC AATGTAGATG AATTGGATGC TGACCTGACT 
TTGGAGAATG ATCCGTCTAA GGGCAGCAAA ACAAAAAAAG GTCCAGCCTA CGCTGGTGGA 
CTGGTCTTGG AGCCAAAGAG AGGCTTATAT GACAAATATG TCTTGCTTCT GGATTTCAAT 
AGTCTTTACC CTTCTATTAT ACAGGAATAC AATATCTGTT TTACGACTAT ACCACGATCA 
GAAGATGGAG TTCCTCGTTT ACCTTCGAGT CAGACACCTG GAATTCTTCC AAAGTTGATG 
GAACATTTGG TCAGTATAAG GAAAAGCGTC AAATTAAAGA TGAAGAAGGA AACAGGTCTC 
AAGTACTGGG AGCTTGACAT TCGACAGCAG GCATTGAAGC TCACAGCCAA TAGTATGTAT 
GGCTGTCTGG GGTTTTCTAA TTCAAGATTC TATGCTAAGC CTTTAGCAGA GCTCATAACA 
CTACAGGGAA GGGATATCCT GCAAAGAACC GTGGATCTTG TCCAGAACCA TTTAAATCTA 
GAGGTAATAT ACGGTGACAC AGATTCAATC ATGATTCACA GCGGACTAGA TGATATTGAA 
GAGGTGAAAG CCATTAAATC AAAAGTCATC CAAGAGGTCA ATAAGAAGTA TAGATGTCTA 
AAAATTGACT GTGATGGCAT ATACAAGAGA ATGCTTCTTC TTCGAAAAAA GAAGTATGCT 
GCTGTCAAGT TGCAGTTTAA GGATGGGAAG CCTTGCGAGG ACATTGAAAG AAAAGGTGTG 
GATATGGTTC GTCGAGATTG GAGCTTACTC TCGAAGGAAA TAGGAGATTT ATGCCTGAGT 
AAAATCTTGT ATGGAGGATC CTGTGAGGAT GTTGTTGAAG CAATTCATAA CGAACTAATG 
AAGATAAAAG AGGAAATGAG AAATGGGCAA GTTGCTCTTG AGAAGTATGT CATCACCAAG 
ACATTGACTA AACCGCCTGC AGCTTATCCA GATTCCAAAA GCCAACCACA CGTTCAAGTT 
GCACTCAGAA TGAGGCAACG TGGTTATAAA GAAGGTTTCA ATGCTAAGGA TACTGTACCG 
TATATAATCT GCTATGAGCA GGGTAATGCT AGCTCTGCTA GTTCAGCAGG AATTGCCGAA 
CGTGCTAGGC ATCCTGATGA GGTGAAATCA GAAGGCAGCA GATGGTTGGT TGACATAGAT 
TACTACTTAG CACAACAGAT TCATCCGGTG GTGTCTCGTC TATGCGCAGA GATTCAAGGA 
ACAAGTCCTG AACGTCTAGC TGAATGTTTA GGACTTGACC CATCAAAGTA CCGAAGCAAA 
TCAAACGATG CTACAAGCAG TGATCCCTCT ACCTCCCTCT TATTCGCAAC AAGCGATGAA 
GAAAGATATA AAAGTTGCGA GCCTTTAGCA TTAACATGCC CCAGCTGCTC TACTGCTTTC 
AACTGTCCAT CTATTATTAG TTCTGTTTGT GCGTCAATCA GTAAGAAACC TGCAACACCT 
GAAACAGAGG AATCTGATTC CACATTCTGG CTTAAACTGC ATTGCCCGAA ATGTCAGCAG 
GAAGATAGCA CGGGAATAAT ATCCCCTGCA ATGATAGCTA ACCAGGTAAA AAGACAGATC 
GATGGATTTG TGTCAATGTA CTACAAAGGC ATAATGGTGT GTGAAGACGA GTCATGCAAG 
CACACAACCC GCTCCCCCAA CTTTCGTCTA CTCGGTGAAC GTGAACGGGG GACAGTTTGC 
CCAAACTATC CTAACTGTAA CGGAACCCTC TTACGAAAGT ACACTGAAGC AGACTTGTAC 
AAGCAACTGT CATACTTCTG CCACATCTTA GACACACAAT GCTCTTTAGA AAAGATGGAT 
GTTGGCGTGA GAATTCAAGT AGAGAAAGCA ATGACGAAGA TAAGACCTGC GGTTAAGTCA 
GCAGCAGCAA TCACTCGAAG TAGTAGAGAC CGGTGTGCTT ATGGATGGAT GCAACTCACA 
GACATAGTCA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ICU2 ( NM_001345811.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001331725.1 Arabidopsis thaliana DNA-directed DNA polymerase (ICU2), mRNA.

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CloneID	OAb22679
Clone ID Related Accession (Same CDS sequence)	NM_126110.3 , NM_001345811.1
Accession Version	NM_001345811.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4575bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	DNA-directed DNA polymerase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076).

ATGTCCGGCG ATAATTCAAC GGAAACTGGA CGAAGACGTA GCCGAGGCGC GGAGGCGTCG 
AGTAGGAAAG ACACACTCGA GCGTCTGAAG GCGATTCGAC AAGGAGGAAT CCGTTCTGCG 
TCAGGTGGTG GGTACGATAT TAGGCTGCAG AAGCCAATCT TCGATACCGT TGATGACGAA 
GAGTACGACG CACTTGTTTC CCGGCGGCGC GAGGAGGCTC GCGGGTTTGT AGTTGAAGAT 
GGGGAAGGTG GCGATTTGGG ATATCTCGAC GAAGGTGAGG AGGAGGATTG GTCGAAGCCA 
AGTGGACCAG AATCGACTGA TGAATCAGAC GACGGTGGTA GGTTTAGTGG GAGACTTAAG 
AAGAAGAAGA AAGGGAAAGA GCAAACGCAG CAACCTCAGG TTAAGAAGGT GAATCCGGCG 
TTAAAAGCTG CGGCTACTAT CACTGGTGAA GGTAGACTTT CGTCTATGTT CACATCTTCG 
AGTTTCAAGA AAGTTAAGGA GACTGATAAG GCACAGTATG AGGGTATTCT TGACGAGATA 
ATAGCTCAGG TTACTCCAGA TGAGTCGGAT AGAAAAAAGC ATACGAGGAG AAAACTACCA 
GGTACTGTTC CAGTTACCAT CTTCAAGAAT AAAAAACTGT TTTCTGTTGC CTCGAGTATG 
GGAATGAAAG AGTCTGAACC AACACCTTCA ACTTATGAGG GTGATTCTGT TTCCATGGAT 
AATGAATTGA TGAAAGAGGA AGATATGAAA GAATCTGAAG TCATTCCTTC TGAGACTATG 
GAATTACTTG GAAGTGATAT TGTCAAAGAG GATGGATCAA ACAAGATAAG GAAAACTGAA 
GTGAAGAGTG AGTTAGGTGT CAAGGAGGTA TTTACATTGA ATGCAACGAT TGATATGAAA 
GAGAAGGATT CGGCTTTAAG TGCCACTGCT GGTTGGAAGG AAGCGATGGG AAAAGTTGGA 
ACTGAGAATG GGGCTTTGCT TGGTTCTAGT TCTGAAGGGA AAACGGAATT TGATTTGGAT 
GCTGACGGAT CACTTCGTTT CTTCATTCTT GACGCTTATG AGGAGGCTTT TGGTGCGAGC 
ATGGGCACAA TATATCTGTT TGGGAAGGTT AAAATGGGAG ATACTTACAA GAGTTGCTGT 
GTGGTAGTTA AGAATATCCA GAGGTGTGTT TATGCTATTC CAAATGATTC CATATTTCCT 
TCCCATGAAC TAATCATGCT CGAGCAAGAG GTGAAAGACT CTCGACTTTC TCCTGAATCC 
TTCCGTGGGA AATTGCATGA AATGGCATCA AAACTGAAAA ACGAAATTGC TCAGGAATTG 
TTACAGCTCA ATGTTTCAAA TTTTAGCATG GCGCCTGTCA AGAGAAACTA TGCTTTTGAG 
AGGCCTGATG TGCCTGCTGG CGAACAATAT GTTTTGAAGA TAAATTATTC ATTTAAGGAT 
CGTCCACTTC CAGAAGATCT TAAAGGCGAG TCTTTCTCCG CTTTGCTCGG GTCTCATACC 
AGTGCTTTGG AGCATTTTAT CCTTAAGAGG AAGATCATGG GACCTTGTTG GCTCAAAATT 
TCAAGCTTCT CTACTTGCTC TCCTTCGGAA GGAGTGAGTT GGTGCAAGTT TGAGGTTACT 
GTTCAATCTC CCAAGGATAT CACTATTTTA GTTTCAGAGG AAAAGGTGGT TCACCCTCCT 
GCTGTTGTTA CAGCCATAAA TTTAAAGACT ATAGTCAATG AAAAGCAGAA CATAAGCGAA 
ATTGTTTCTG CATCTGTTCT CTGCTTTCAC AACGCCAAGA TTGATGTTCC AATGCCAGCT 
CCAGAAAGAA AGAGATCTGG TATTCTGAGT CATTTCACTG TTGTTAGGAA TCCTGAAGGA 
ACCGGTTACC CAATTGGTTG GAAAAAAGAA GTGTCTGACA GAAATTCAAA GAACGGCTGC 
AATGTATTAA GTATTGAAAA CAGTGAAAGA GCATTGCTGA ACCGATTGTT TTTGGAGTTA 
AACAAATTGG ACAGTGATAT TCTTGTGGGA CATAATATTT CAGGTTTTGA TCTGGATGTT 
CTTCTTCAAA GGGCCCAGGC TTGCAAAGTT CAGAGTAGCA TGTGGTCCAA AATTGGCCGA 
CTCAAACGCT CTTTTATGCC TAAGCTTAAA GGAAACAGTA ATTATGGGTC AGGAGCTACA 
CCAGGGCTCA TGTCTTGTAT TGCTGGAAGG CTCTTGTGTG ATACAGATCT GTGTTCTCGT 
GACTTGTTGA AGGAGGTCAG TTATTCTTTA ACGGACCTCT CAAAGACACA GCTGAACCGG 
GACAGGAAAG AGATAGCGCC TAATGATATT CCCAAAATGT TTCAGTCATC CAAAACACTT 
GTGGAACTTA TTGAATGTGG TGAGACGGAT GCATGGTTAT CCATGGAGCT CATGTTTCAT 
CTAAGTGTTC TTCCTCTCAC TCTCCAGCTT ACTAACATAA GTGGCAATCT TTGGGGGAAA 
ACACTTCAAG GAGCCAGGGC ACAGAGAATC GAATACTACT TACTACATAC ATTCCACTCG 
AAAAAGTTCA TTCTCCCTGA CAAAATTTCA CAACGTATGA AGGAAATAAA GTCATCAAAA 
AGAAGAATGG ATTATGCTCC AGAAGACCGC AATGTAGATG AATTGGATGC TGACCTGACT 
TTGGAGAATG ATCCGTCTAA GGGCAGCAAA ACAAAAAAAG GTCCAGCCTA CGCTGGTGGA 
CTGGTCTTGG AGCCAAAGAG AGGCTTATAT GACAAATATG TCTTGCTTCT GGATTTCAAT 
AGTCTTTACC CTTCTATTAT ACAGGAATAC AATATCTGTT TTACGACTAT ACCACGATCA 
GAAGATGGAG TTCCTCGTTT ACCTTCGAGT CAGACACCTG GAATTCTTCC AAAGTTGATG 
GAACATTTGG TCAGTATAAG GAAAAGCGTC AAATTAAAGA TGAAGAAGGA AACAGGTCTC 
AAGTACTGGG AGCTTGACAT TCGACAGCAG GCATTGAAGC TCACAGCCAA TAGTATGTAT 
GGCTGTCTGG GGTTTTCTAA TTCAAGATTC TATGCTAAGC CTTTAGCAGA GCTCATAACA 
CTACAGGGAA GGGATATCCT GCAAAGAACC GTGGATCTTG TCCAGAACCA TTTAAATCTA 
GAGGTAATAT ACGGTGACAC AGATTCAATC ATGATTCACA GCGGACTAGA TGATATTGAA 
GAGGTGAAAG CCATTAAATC AAAAGTCATC CAAGAGGTCA ATAAGAAGTA TAGATGTCTA 
AAAATTGACT GTGATGGCAT ATACAAGAGA ATGCTTCTTC TTCGAAAAAA GAAGTATGCT 
GCTGTCAAGT TGCAGTTTAA GGATGGGAAG CCTTGCGAGG ACATTGAAAG AAAAGGTGTG 
GATATGGTTC GTCGAGATTG GAGCTTACTC TCGAAGGAAA TAGGAGATTT ATGCCTGAGT 
AAAATCTTGT ATGGAGGATC CTGTGAGGAT GTTGTTGAAG CAATTCATAA CGAACTAATG 
AAGATAAAAG AGGAAATGAG AAATGGGCAA GTTGCTCTTG AGAAGTATGT CATCACCAAG 
ACATTGACTA AACCGCCTGC AGCTTATCCA GATTCCAAAA GCCAACCACA CGTTCAAGTT 
GCACTCAGAA TGAGGCAACG TGGTTATAAA GAAGGTTTCA ATGCTAAGGA TACTGTACCG 
TATATAATCT GCTATGAGCA GGGTAATGCT AGCTCTGCTA GTTCAGCAGG AATTGCCGAA 
CGTGCTAGGC ATCCTGATGA GGTGAAATCA GAAGGCAGCA GATGGTTGGT TGACATAGAT 
TACTACTTAG CACAACAGAT TCATCCGGTG GTGTCTCGTC TATGCGCAGA GATTCAAGGA 
ACAAGTCCTG AACGTCTAGC TGAATGTTTA GGACTTGACC CATCAAAGTA CCGAAGCAAA 
TCAAACGATG CTACAAGCAG TGATCCCTCT ACCTCCCTCT TATTCGCAAC AAGCGATGAA 
GAAAGATATA AAAGTTGCGA GCCTTTAGCA TTAACATGCC CCAGCTGCTC TACTGCTTTC 
AACTGTCCAT CTATTATTAG TTCTGTTTGT GCGTCAATCA GTAAGAAACC TGCAACACCT 
GAAACAGAGG AATCTGATTC CACATTCTGG CTTAAACTGC ATTGCCCGAA ATGTCAGCAG 
GAAGATAGCA CGGGAATAAT ATCCCCTGCA ATGATAGCTA ACCAGGTAAA AAGACAGATC 
GATGGATTTG TGTCAATGTA CTACAAAGGC ATAATGGTGT GTGAAGACGA GTCATGCAAG 
CACACAACCC GCTCCCCCAA CTTTCGTCTA CTCGGTGAAC GTGAACGGGG GACAGTTTGC 
CCAAACTATC CTAACTGTAA CGGAACCCTC TTACGAAAGT ACACTGAAGC AGACTTGTAC 
AAGCAACTGT CATACTTCTG CCACATCTTA GACACACAAT GCTCTTTAGA AAAGATGGAT 
GTTGGCGTGA GAATTCAAGT AGAGAAAGCA ATGACGAAGA TAAGACCTGC GGTTAAGTCA 
GCAGCAGCAA TCACTCGAAG TAGTAGAGAC CGGTGTGCTT ATGGATGGAT GCAACTCACA 
GACATAGTCA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001331725.1
CDS	42..4616
Translation	MSGDNSTETGRRRSRGAEASSRKDTLERLKAIRQGGIRSASGGGYDIRLQKPIFDTVDDEEYDALVSRRREEARGFVVEDGEGGDLGYLDEGEEEDWSKPSGPESTDESDDGGRFSGRLKKKKKGKEQTQQPQVKKVNPALKAAATITGEGRLSSMFTSSSFKKVKETDKAQYEGILDEIIAQVTPDESDRKKHTRRKLPGTVPVTIFKNKKLFSVASSMGMKESEPTPSTYEGDSVSMDNELMKEEDMKESEVIPSETMELLGSDIVKEDGSNKIRKTEVKSELGVKEVFTLNATIDMKEKDSALSATAGWKEAMGKVGTENGALLGSSSEGKTEFDLDADGSLRFFILDAYEEAFGASMGTIYLFGKVKMGDTYKSCCVVVKNIQRCVYAIPNDSIFPSHELIMLEQEVKDSRLSPESFRGKLHEMASKLKNEIAQELLQLNVSNFSMAPVKRNYAFERPDVPAGEQYVLKINYSFKDRPLPEDLKGESFSALLGSHTSALEHFILKRKIMGPCWLKISSFSTCSPSEGVSWCKFEVTVQSPKDITILVSEEKVVHPPAVVTAINLKTIVNEKQNISEIVSASVLCFHNAKIDVPMPAPERKRSGILSHFTVVRNPEGTGYPIGWKKEVSDRNSKNGCNVLSIENSERALLNRLFLELNKLDSDILVGHNISGFDLDVLLQRAQACKVQSSMWSKIGRLKRSFMPKLKGNSNYGSGATPGLMSCIAGRLLCDTDLCSRDLLKEVSYSLTDLSKTQLNRDRKEIAPNDIPKMFQSSKTLVELIECGETDAWLSMELMFHLSVLPLTLQLTNISGNLWGKTLQGARAQRIEYYLLHTFHSKKFILPDKISQRMKEIKSSKRRMDYAPEDRNVDELDADLTLENDPSKGSKTKKGPAYAGGLVLEPKRGLYDKYVLLLDFNSLYPSIIQEYNICFTTIPRSEDGVPRLPSSQTPGILPKLMEHLVSIRKSVKLKMKKETGLKYWELDIRQQALKLTANSMYGCLGFSNSRFYAKPLAELITLQGRDILQRTVDLVQNHLNLEVIYGDTDSIMIHSGLDDIEEVKAIKSKVIQEVNKKYRCLKIDCDGIYKRMLLLRKKKYAAVKLQFKDGKPCEDIERKGVDMVRRDWSLLSKEIGDLCLSKILYGGSCEDVVEAIHNELMKIKEEMRNGQVALEKYVITKTLTKPPAAYPDSKSQPHVQVALRMRQRGYKEGFNAKDTVPYIICYEQGNASSASSAGIAERARHPDEVKSEGSRWLVDIDYYLAQQIHPVVSRLCAEIQGTSPERLAECLGLDPSKYRSKSNDATSSDPSTSLLFATSDEERYKSCEPLALTCPSCSTAFNCPSIISSVCASISKKPATPETEESDSTFWLKLHCPKCQQEDSTGIISPAMIANQVKRQIDGFVSMYYKGIMVCEDESCKHTTRSPNFRLLGERERGTVCPNYPNCNGTLLRKYTEADLYKQLSYFCHILDTQCSLEKMDVGVRIQVEKAMTKIRPAVKSAAAITRSSRDRCAYGWMQLTDIVI

Target ORF information:

RefSeq Version	NM_001345811.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana DNA-directed DNA polymerase (ICU2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001345811.1

ORF Insert Sequence:

ATGTCCGGCG ATAATTCAAC GGAAACTGGA CGAAGACGTA GCCGAGGCGC GGAGGCGTCG 
AGTAGGAAAG ACACACTCGA GCGTCTGAAG GCGATTCGAC AAGGAGGAAT CCGTTCTGCG 
TCAGGTGGTG GGTACGATAT TAGGCTGCAG AAGCCAATCT TCGATACCGT TGATGACGAA 
GAGTACGACG CACTTGTTTC CCGGCGGCGC GAGGAGGCTC GCGGGTTTGT AGTTGAAGAT 
GGGGAAGGTG GCGATTTGGG ATATCTCGAC GAAGGTGAGG AGGAGGATTG GTCGAAGCCA 
AGTGGACCAG AATCGACTGA TGAATCAGAC GACGGTGGTA GGTTTAGTGG GAGACTTAAG 
AAGAAGAAGA AAGGGAAAGA GCAAACGCAG CAACCTCAGG TTAAGAAGGT GAATCCGGCG 
TTAAAAGCTG CGGCTACTAT CACTGGTGAA GGTAGACTTT CGTCTATGTT CACATCTTCG 
AGTTTCAAGA AAGTTAAGGA GACTGATAAG GCACAGTATG AGGGTATTCT TGACGAGATA 
ATAGCTCAGG TTACTCCAGA TGAGTCGGAT AGAAAAAAGC ATACGAGGAG AAAACTACCA 
GGTACTGTTC CAGTTACCAT CTTCAAGAAT AAAAAACTGT TTTCTGTTGC CTCGAGTATG 
GGAATGAAAG AGTCTGAACC AACACCTTCA ACTTATGAGG GTGATTCTGT TTCCATGGAT 
AATGAATTGA TGAAAGAGGA AGATATGAAA GAATCTGAAG TCATTCCTTC TGAGACTATG 
GAATTACTTG GAAGTGATAT TGTCAAAGAG GATGGATCAA ACAAGATAAG GAAAACTGAA 
GTGAAGAGTG AGTTAGGTGT CAAGGAGGTA TTTACATTGA ATGCAACGAT TGATATGAAA 
GAGAAGGATT CGGCTTTAAG TGCCACTGCT GGTTGGAAGG AAGCGATGGG AAAAGTTGGA 
ACTGAGAATG GGGCTTTGCT TGGTTCTAGT TCTGAAGGGA AAACGGAATT TGATTTGGAT 
GCTGACGGAT CACTTCGTTT CTTCATTCTT GACGCTTATG AGGAGGCTTT TGGTGCGAGC 
ATGGGCACAA TATATCTGTT TGGGAAGGTT AAAATGGGAG ATACTTACAA GAGTTGCTGT 
GTGGTAGTTA AGAATATCCA GAGGTGTGTT TATGCTATTC CAAATGATTC CATATTTCCT 
TCCCATGAAC TAATCATGCT CGAGCAAGAG GTGAAAGACT CTCGACTTTC TCCTGAATCC 
TTCCGTGGGA AATTGCATGA AATGGCATCA AAACTGAAAA ACGAAATTGC TCAGGAATTG 
TTACAGCTCA ATGTTTCAAA TTTTAGCATG GCGCCTGTCA AGAGAAACTA TGCTTTTGAG 
AGGCCTGATG TGCCTGCTGG CGAACAATAT GTTTTGAAGA TAAATTATTC ATTTAAGGAT 
CGTCCACTTC CAGAAGATCT TAAAGGCGAG TCTTTCTCCG CTTTGCTCGG GTCTCATACC 
AGTGCTTTGG AGCATTTTAT CCTTAAGAGG AAGATCATGG GACCTTGTTG GCTCAAAATT 
TCAAGCTTCT CTACTTGCTC TCCTTCGGAA GGAGTGAGTT GGTGCAAGTT TGAGGTTACT 
GTTCAATCTC CCAAGGATAT CACTATTTTA GTTTCAGAGG AAAAGGTGGT TCACCCTCCT 
GCTGTTGTTA CAGCCATAAA TTTAAAGACT ATAGTCAATG AAAAGCAGAA CATAAGCGAA 
ATTGTTTCTG CATCTGTTCT CTGCTTTCAC AACGCCAAGA TTGATGTTCC AATGCCAGCT 
CCAGAAAGAA AGAGATCTGG TATTCTGAGT CATTTCACTG TTGTTAGGAA TCCTGAAGGA 
ACCGGTTACC CAATTGGTTG GAAAAAAGAA GTGTCTGACA GAAATTCAAA GAACGGCTGC 
AATGTATTAA GTATTGAAAA CAGTGAAAGA GCATTGCTGA ACCGATTGTT TTTGGAGTTA 
AACAAATTGG ACAGTGATAT TCTTGTGGGA CATAATATTT CAGGTTTTGA TCTGGATGTT 
CTTCTTCAAA GGGCCCAGGC TTGCAAAGTT CAGAGTAGCA TGTGGTCCAA AATTGGCCGA 
CTCAAACGCT CTTTTATGCC TAAGCTTAAA GGAAACAGTA ATTATGGGTC AGGAGCTACA 
CCAGGGCTCA TGTCTTGTAT TGCTGGAAGG CTCTTGTGTG ATACAGATCT GTGTTCTCGT 
GACTTGTTGA AGGAGGTCAG TTATTCTTTA ACGGACCTCT CAAAGACACA GCTGAACCGG 
GACAGGAAAG AGATAGCGCC TAATGATATT CCCAAAATGT TTCAGTCATC CAAAACACTT 
GTGGAACTTA TTGAATGTGG TGAGACGGAT GCATGGTTAT CCATGGAGCT CATGTTTCAT 
CTAAGTGTTC TTCCTCTCAC TCTCCAGCTT ACTAACATAA GTGGCAATCT TTGGGGGAAA 
ACACTTCAAG GAGCCAGGGC ACAGAGAATC GAATACTACT TACTACATAC ATTCCACTCG 
AAAAAGTTCA TTCTCCCTGA CAAAATTTCA CAACGTATGA AGGAAATAAA GTCATCAAAA 
AGAAGAATGG ATTATGCTCC AGAAGACCGC AATGTAGATG AATTGGATGC TGACCTGACT 
TTGGAGAATG ATCCGTCTAA GGGCAGCAAA ACAAAAAAAG GTCCAGCCTA CGCTGGTGGA 
CTGGTCTTGG AGCCAAAGAG AGGCTTATAT GACAAATATG TCTTGCTTCT GGATTTCAAT 
AGTCTTTACC CTTCTATTAT ACAGGAATAC AATATCTGTT TTACGACTAT ACCACGATCA 
GAAGATGGAG TTCCTCGTTT ACCTTCGAGT CAGACACCTG GAATTCTTCC AAAGTTGATG 
GAACATTTGG TCAGTATAAG GAAAAGCGTC AAATTAAAGA TGAAGAAGGA AACAGGTCTC 
AAGTACTGGG AGCTTGACAT TCGACAGCAG GCATTGAAGC TCACAGCCAA TAGTATGTAT 
GGCTGTCTGG GGTTTTCTAA TTCAAGATTC TATGCTAAGC CTTTAGCAGA GCTCATAACA 
CTACAGGGAA GGGATATCCT GCAAAGAACC GTGGATCTTG TCCAGAACCA TTTAAATCTA 
GAGGTAATAT ACGGTGACAC AGATTCAATC ATGATTCACA GCGGACTAGA TGATATTGAA 
GAGGTGAAAG CCATTAAATC AAAAGTCATC CAAGAGGTCA ATAAGAAGTA TAGATGTCTA 
AAAATTGACT GTGATGGCAT ATACAAGAGA ATGCTTCTTC TTCGAAAAAA GAAGTATGCT 
GCTGTCAAGT TGCAGTTTAA GGATGGGAAG CCTTGCGAGG ACATTGAAAG AAAAGGTGTG 
GATATGGTTC GTCGAGATTG GAGCTTACTC TCGAAGGAAA TAGGAGATTT ATGCCTGAGT 
AAAATCTTGT ATGGAGGATC CTGTGAGGAT GTTGTTGAAG CAATTCATAA CGAACTAATG 
AAGATAAAAG AGGAAATGAG AAATGGGCAA GTTGCTCTTG AGAAGTATGT CATCACCAAG 
ACATTGACTA AACCGCCTGC AGCTTATCCA GATTCCAAAA GCCAACCACA CGTTCAAGTT 
GCACTCAGAA TGAGGCAACG TGGTTATAAA GAAGGTTTCA ATGCTAAGGA TACTGTACCG 
TATATAATCT GCTATGAGCA GGGTAATGCT AGCTCTGCTA GTTCAGCAGG AATTGCCGAA 
CGTGCTAGGC ATCCTGATGA GGTGAAATCA GAAGGCAGCA GATGGTTGGT TGACATAGAT 
TACTACTTAG CACAACAGAT TCATCCGGTG GTGTCTCGTC TATGCGCAGA GATTCAAGGA 
ACAAGTCCTG AACGTCTAGC TGAATGTTTA GGACTTGACC CATCAAAGTA CCGAAGCAAA 
TCAAACGATG CTACAAGCAG TGATCCCTCT ACCTCCCTCT TATTCGCAAC AAGCGATGAA 
GAAAGATATA AAAGTTGCGA GCCTTTAGCA TTAACATGCC CCAGCTGCTC TACTGCTTTC 
AACTGTCCAT CTATTATTAG TTCTGTTTGT GCGTCAATCA GTAAGAAACC TGCAACACCT 
GAAACAGAGG AATCTGATTC CACATTCTGG CTTAAACTGC ATTGCCCGAA ATGTCAGCAG 
GAAGATAGCA CGGGAATAAT ATCCCCTGCA ATGATAGCTA ACCAGGTAAA AAGACAGATC 
GATGGATTTG TGTCAATGTA CTACAAAGGC ATAATGGTGT GTGAAGACGA GTCATGCAAG 
CACACAACCC GCTCCCCCAA CTTTCGTCTA CTCGGTGAAC GTGAACGGGG GACAGTTTGC 
CCAAACTATC CTAACTGTAA CGGAACCCTC TTACGAAAGT ACACTGAAGC AGACTTGTAC 
AAGCAACTGT CATACTTCTG CCACATCTTA GACACACAAT GCTCTTTAGA AAAGATGGAT 
GTTGGCGTGA GAATTCAAGT AGAGAAAGCA ATGACGAAGA TAAGACCTGC GGTTAAGTCA 
GCAGCAGCAA TCACTCGAAG TAGTAGAGAC CGGTGTGCTT ATGGATGGAT GCAACTCACA 
GACATAGTCA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Nature408(6814)823-6(2000 Dec)
Tabata S,Kaneko T,Nakamura Y,Kotani H,Kato T,Asamizu E,Miyajima N,Sasamoto S,Kimura T,Hosouchi T,Kawashima K,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Naruo K,Okumura S,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Sato S,de la Bastide M,Huang E,Spiegel L,Gnoj L,O'Shaughnessy A,Preston R,Habermann K,Murray J,Johnson D,Rohlfing T,Nelson J,Stoneking T,Pepin K,Spieth J,Sekhon M,Armstrong J,Becker M,Belter E,Cordum H,Cordes M,Courtney L,Courtney W,Dante M,Du H,Edwards J,Fryman J,Haakensen B,Lamar E,Latreille P,Leonard S,Meyer R,Mulvaney E,Ozersky P,Riley A,Strowmatt C,Wagner-McPherson C,Wollam A,Yoakum M,Bell M,Dedhia N,Parnell L,Shah R,Rodriguez M,See LH,Vil D,Baker J,Kirchoff K,Toth K,King L,Bahret A,Miller B,Marra M,Martienssen R,McCombie WR,Wilson RK,Murphy G,Bancroft I,Volckaert G,Wambutt R,D?sterh?ft A,Stiekema W,Pohl T,Entian KD,Terryn N,Hartley N,Bent E,Johnson S,Langham SA,McCullagh B,Robben J,Grymonprez B,Zimmermann W,Ramsperger U,Wedler H,Balke K,Wedler E,Peters S,van Staveren M,Dirkse W,Mooijman P,Lankhorst RK,Weitzenegger T,Bothe G,Rose M,Hauf J,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Villarroel R,Gielen J,Ardiles W,Bents O,Lemcke K,Kolesov G,Mayer K,Rudd S,Schoof H,Schueller C,Zaccaria P,Mewes HW,Bevan M,Fransz P,,,

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ICU2 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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