Pola1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pola1
Entrez Gene ID	85241
Full Name	DNA polymerase alpha 1, catalytic subunit
General protein information	Preferred Names DNA polymerase alpha 1, catalytic subunit Names DNA polymerase alpha catalytic subunit DNA polymerase alpha catalytic subunit p180 DNA polymerase alpha subunit I polymerase (DNA directed), alpha 1, catalytic subunit polymerase (DNA) alpha 1, catalytic subunit
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: X Map Location: Xq22
Summary	exhibits DNA polymerase alpha activity; may mediate DNA replication process [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053479.1	NP_445931.1	DNA polymerase alpha catalytic subunit

Pathways from BioSystems

Homologies

	POLA1	XP_001091195.1
Gallus gallus (chicken)	POLA1	XP_416792.3
Eremothecium gossypii	AGOS_AER381C	NP_985237.1
Homo sapiens (human)	POLA1	NP_058633.2
Rattus norvegicus (Norway rat)	Pola1	NP_445931.1
Schizosaccharomyces pombe (fission yeast)	pol1	NP_594186.1
Pan troglodytes (chimpanzee)	POLA1	XP_003317445.2
Mus musculus (house mouse)	Pola1	NP_032918.1
Danio rerio (zebrafish)	pola1	XP_005162809.1
Drosophila melanogaster (fruit fly)	DNApol-alpha180	NP_536736.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001929	XP_321134.5
Caenorhabditis elegans (roundworm)	Y47D3A.29	NP_001255109.1
Magnaporthe oryzae (rice blast fungus)	MGG_06397	XP_003717174.1
Neurospora crassa	NCU07870	XP_963131.2
Arabidopsis thaliana (thale cress)	ICU2	NP_201511.2
Xenopus tropicalis (tropical clawed frog)	pola1	XP_004911812.1
Bos taurus (cattle)	POLA1	NP_001192994.1
Saccharomyces cerevisiae (baker's yeast)	POL1	NP_014297.3
Kluyveromyces lactis	KLLA0C13255g	XP_452791.1
Canis lupus familiaris (dog)	POLA1	XP_005641271.1
Oryza sativa (rice)	Os01g0868300	NP_001044920.1

Gene Ontology
Bibliography

Related articles in PubMed

DNA polymerase ? and ? switch by sharing accessory subunits of DNA polymerase ?.
Baranovskiy AG, Lada AG, Siebler HM, Zhang Y, Pavlov YI, Tahirov TH
The Journal of biological chemistry287(21)17281-7(2012 May)

Distinctive activities of DNA polymerases during human DNA replication.
Rytk?nen AK, Vaara M, Nethanel T, Kaufmann G, Sormunen R, L??r? E, Nasheuer HP, Rahmeh A, Lee MY, Syv?oja JE, Pospiech H
The FEBS journal273(13)2984-3001(2006 Jul)

Transcription factor YB-1 mediates DNA polymerase alpha gene expression.
En-Nia A, Yilmaz E, Klinge U, Lovett DH, Stefanidis I, Mertens PR
The Journal of biological chemistry280(9)7702-11(2005 Mar)

The role of DNA polymerase activity in human non-homologous end joining.
Pospiech H, Rytk?nen AK, Syv?oja JE
Nucleic acids research29(15)3277-88(2001 Aug)

A mutation in subunit B of the DNA polymerase alpha-primase complex from Novikoff hepatoma cells concomitant with a conformational change and abnormal catalytic properties of the DNA polymerase alpha-primase complex.
Popanda O, Flohr C, Dai JC, Hunzicker A, Thielmann HW
Molecular carcinogenesis31(4)171-83(2001 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

YB-1 mediates DNA polymerase alpha gene expression
Title: Transcription factor YB-1 mediates DNA polymerase alpha gene expression.

The following Pola1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pola1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa42810	NM_053479.1 Latest version!	Rattus norvegicus DNA polymerase alpha 1, catalytic subunit (Pola1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pola1 ( NM_053479.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_445931.1 Rattus norvegicus DNA polymerase alpha 1, catalytic subunit (Pola1), mRNA.

Price & Availability↑

CloneID	ORa42810
Clone ID Related Accession (Same CDS sequence)	NM_053479.1
Accession Version	NM_053479.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4407bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	DNA polymerase alpha catalytic subunit
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AJ011605.1, FM076387.1, CN543332.1 and AI511322.1. On or before May 21, 2010 this sequence version replaced XM_001064735.2, XM_242396.6. Sequence Note: This RefSeq record was created from transcripts from several strains because no full length transcript from a single strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ011605.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGCCTG TTCACGGAGA CGACTGCAAA CTGGAAACAA GCGCTGTGTC AGATTCAGGG 
AGTTTTGTAG CTTCTCGAGC ACGTCGAGAA AAAAAATCAA AGAAAGGACG CCAAGAAGCT 
TTAGAGCGAC TGAAAAAGGC TAAAGCTGGT GAGAAGTACA AGTATGAAGT TGAGGACCTC 
ACAAGTGTTT ATGAAGAAGT TGATGAAGAG CAGTATTCAA AACTGGTTCA GGCACGTCAG 
GATGATGACT GGATCGTGGA TGACGACGGT ATAGGCTATG TGGAAGACGG TAGAGAGATT 
TTTGATGACG ACCTTGAAGA TGATGCACTT GACACCTGTG GGGAAGGAAG TGATGGCAAA 
GCACACAGAA AGGACAGGAA GGATGTAAAG AAACCTTCAG TGACAAAACC AAACAATATC 
AAGGCAATGT TCATTGCTAG TGCTGGGAAG AAAACCACAG ATAAAACAGT TGACTTGTCC 
AAGGATGATC TGCTAGGTGA CATTCTACAG GATCTGAACA CTGAGACACC TCAAATAGCT 
CCACCGCCAG TATTGATACC AAAGAAAAAA AGATCTACTG GAGCTTCACC GAATCCTTTC 
TCTGTGCACA CCGCCACGGC AGTTCCTTCA GGAAAAATTG CTTCCCCTGT CTCCAGAAAG 
GAGCCTCCAT TAACTCCTGT TCCTCTTAAA CGTGCTGAAT TTGCTGGCGA TCTGGCTCAG 
CCAGAGTGTC CAGAAGATGA GCAGGAGTCA GGGGTTATAG AGTTTGAAGA TGGCGACTTT 
GATGAGCCCA TGGACACAGA GGAAGTAGAT GAGGAGGAGC CTGTGACTGC CAAGATTTGG 
GACCAAGAGA GTGAGCCAGT GGAAGGGGTG AAGCATGAGG CAGATCCTGA GACGGGAACC 
ACATCTTTCT TAGACAGTTT TCTCCCAGAT GTTTCTTGTT GGGACATTGA TCAGAAGGAC 
GAAAACAGTT TCTTACTTCA AGAAGTTCAA GTGGACTCCA ATCATCTCCC GTTGGTAAAA 
GGGGCAGATG ATGAACAAGT CTTTCAGTTT TATTGGCTGG ATGCTTATGA AGATCCATAC 
AACCAACCAG GTGTGGTTTT TCTGTTTGGC AAAGTATGGG TCGAATCAGC CAAAACCCAT 
GTGAGCTGTT GTGTTATGGT GAAAAATATT GAGCGAACAC TCTACTTCCT TCCCCGTGAA 
ATGAAAATTG ACCTAAACAC AGGGAAAGAA ACAGCAACTC CAATTACAAT GAAGGATGTT 
TATGAAGAAT TTGATTCAAA AATTTCAGCA AAATATAAAA TAATGAAGTT CAAGTCCAAG 
ATAGTGGAAA AGAACTATGC TTTTGAGATA CCTGATGTTC CAGAAAAATC TGAGTACTTG 
GAAGTTAGAT ACTCGGCAGA AGTGCCACAG CTTCCACAGA ATTTAAAAGG AGAAACATTT 
TCTCATGTAT TTGGAACTAA CACGTCTAGC TTGGAACTGT TCTTGATGAA TAGAAAGATC 
AAAGGACCCT GTTGGCTTGA AGTAAAAAAT CCACAACTCT TGAATCAACC AATCAGTTGG 
TGTAAATTTG AGGCAATGGC TTTGAAACCA GACTTGGTGA ATGTAATTAA GGATGTCAGT 
CCTCCTCCAC TTGTGGTGAT GTCTTTCAGC ATGAAGACAA TGCAAAATGT ACAGAACCAT 
CAACACGAGA TCATTGCTAT GGCAGCGCTG GTTCATCACA ATTTCCCATT AGATAAAGCA 
CCTCCAAAAC CTCCCTTTCA GACGCATTTC TGTGTTGTGT CTAAACCAAA GGACTGTATT 
TTCCCATGCG CTTTCAAAGA AGTCATTAAG AAAAAGAATA TGGAGGTTGA GGTTGCTGCA 
ACAGAAAGAA CTTTGCTAGG ATTTTTCCTT GCAAAAGTTC ACAAACTTGA CCCTGATATT 
CTCGTGGGTC ATAATATTTG TGGTTTTGAA CTAGAAGTGC TACTGCAAAG AATTAATGAG 
TGCAAGGTTC CTTTCTGGTC CAAGATAGGT CGGCTGAGGA GATCCAACAT GCCAAAACTT 
GGGAGCCGGA GTGGATTTGG TGAAAGAAAT GCTACCTGTG GTCGAATGAT ATGTGATGTG 
GAAATTTCAG TAAAAGAATT GATTCATTGT AAAAGTTACC ATTTGTCTGA ACTTGTTCAG 
CAGATTCTGA AAACTGAGAG GATTGTAATT CCAACAGAAA ATATACGAAA CATGTATAGT 
GAACCTTCTC ATCTGTTGTA CCTGTTGGAA CACATCTGGA AAGATGCCAG ATTCATTTTG 
CAGATCATGT GTGAGCTAAA TGTTCTTCCA TTAGCATTGC AGATCACTAA CATCGCTGGG 
AACATTATGT CTAGGACACT GATGGGTGGA CGATCTGAGC GAAACGAGTT TTTGTTGCTT 
CATGCATTTT ATGAAAACAA CTATATTGTG CCTGACAAGC AGATTTTCAG AAAACCTCAG 
CAAAAACCGG GAGATGAAGA TGAAGAAATT GATGGGGATA CCAATAAATA TAAGAAAGGA 
CGCAAGAAAG CAGCTTATGC TGGAGGCTTG GTTTTGGACC CCAAAGTTGG TTTTTATGAC 
AAGTTCATTT TGCTCCTGGA TTTCAACAGT TTATATCCTT CCATCATTCA GGAATTTAAC 
ATTTGTTTTA CAACAGTGCA AAGAGTTGCT TCAGAGACAC TGAAAGCTAC AGAGGATGAG 
GAACAAGAAC AGATTCCAGA GTTGCCAGAT CCAAACTTAG ATATGGGCAT TTTGCCCAGA 
GAGATCCGGA AACTGGTAGA GCGGAGAAAA CAAGTGAAAC AGCTAATGAA ACAGCAAGAT 
TTAAATCCAG ACCTTGTTCT TCAGTATGAT ATCCGACAGA AGGCTTTGAA GCTCACAGCG 
AACAGTATGT ATGGTTGCCT GGGATTTTCG TACAGCAGAT TTTATGCTAA ACCGCTGGCT 
GCCTTGGTGA CATACAAAGG AAGGGAGATT TTGATGCACA CGAAAGAGAT GGTACAGAAG 
ATGAATCTTG AAGTTATTTA TGGAGACACA GATTCAATTA TGATAAATAC CAATAGCACC 
AACCTGGAAG AAGTCTTTAA GTTGGGAAAC AAGGTAAAAA ATGAAGTGAA TAAATTGTAC 
AAACTGCTTG AAATAGACAT TGATGGTGTT TTCAAGTCTC TGCTATTGCT GAAGAAAAAG 
AAATATGCTG CTCTAGTTGT CGAACCAACA TCCGATGGGA ATTACATCAC CAAGCAGGAA 
CTGAAAGGAT TGGATATAGT TAGAAGGGAC TGGTGTGATC TTGCCAAGGA CACCGGAAAC 
TTTGTCATTG GCCAGATTCT TTCTGATCAA AGTCGGGACA CCATTGTGGA AAACATTCAG 
AAGAGGTTAA TAGAAATTGG AGAAAATGTG CTAAACGGCA GTGTCCCAGT GAGCCAGTTT 
GAAATTAACA AGGCATTGAC AAAGGATCCC CAGGATTACC CTGATAAAAA AAGTCTACCT 
CATGTACATG TTGCACTGTG GATAAATTCT CAAGGAGGCA GGAAGGTGAA AGCTGGCGAT 
ACAGTGTCAT ATGTTATCTG TCAGGATGGG TCAAACCTCC CTGCAACTCA GAGGGCCTAT 
GCACCCGAGC AATTGCAGAA ACAGGACAAT TTAGCCATCG ACACCCAGTA CTACTTGGCC 
CAGCAGATCC ACCCTGTTGT GGCTCGAATC TGTGAGCCAA TAGATGGAAT TGATGCTGTA 
CTCATTGCAT TATGGCTAGG ACTTGACTCC ACTCAATTTA GAGTTCATCA GTATCACAAA 
GATGAAGAGA ATGATGCTCT CCTTGGTGGT CCAGCACAGC TAACTGATGA AGAGAAATAC 
AAGGATTGTG AAAAATTCAA ATGCCTTTGT CCTTCATGTG GAACTGAAAA TATTTATGAT 
AATGTCTTTG AAGGCTCGGG AATGGATATG GAGCCCAGCT TGAATCGTTG CAGTAATATT 
GATTGTAAGG CTTCACCAGC GACCTTTATG GTACAGTTGA GCAACAAATT GATCATGGAC 
ATTAGACGTT GCATTAAAAA GTACTATGAT GGCTGGTTGA TATGTGAGGA GCCAACTTGT 
CGCAATCGCA TTCGTCGTCT TCCCCTTCAT TTCTCCCGAA ATGGGCCTCT GTGTCCAGCC 
TGCATGAAAG CTGTGCTCAG ACCAGAGTAT TCTGACAAGT CCCTGTACAC CCAGCTGTGC 
TTTTACCGGT ACATTTTTGA TGCGGACTGT GCACTGGAAA AACTTCCTGA ACACGAGAAA 
GATAAATTGA AGAAGCAGTT TTTCACTCCC AGAGTTCTTC AGGACTACAG AAAAGTCAAG 
AACATAGCAG AACACTTCTT GTCCTGGAGT GGCTACTCTG AAGTAAACCT CAGCAAGCTC 
TTCGCCAATT ATGCTGGGAA GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_445931.1
CDS	11..4417
Translation	MAPVHGDDCKLETSAVSDSGSFVASRARREKKSKKGRQEALERLKKAKAGEKYKYEVEDLTSVYEEVDEEQYSKLVQARQDDDWIVDDDGIGYVEDGREIFDDDLEDDALDTCGEGSDGKAHRKDRKDVKKPSVTKPNNIKAMFIASAGKKTTDKTVDLSKDDLLGDILQDLNTETPQIAPPPVLIPKKKRSTGASPNPFSVHTATAVPSGKIASPVSRKEPPLTPVPLKRAEFAGDLAQPECPEDEQESGVIEFEDGDFDEPMDTEEVDEEEPVTAKIWDQESEPVEGVKHEADPETGTTSFLDSFLPDVSCWDIDQKDENSFLLQEVQVDSNHLPLVKGADDEQVFQFYWLDAYEDPYNQPGVVFLFGKVWVESAKTHVSCCVMVKNIERTLYFLPREMKIDLNTGKETATPITMKDVYEEFDSKISAKYKIMKFKSKIVEKNYAFEIPDVPEKSEYLEVRYSAEVPQLPQNLKGETFSHVFGTNTSSLELFLMNRKIKGPCWLEVKNPQLLNQPISWCKFEAMALKPDLVNVIKDVSPPPLVVMSFSMKTMQNVQNHQHEIIAMAALVHHNFPLDKAPPKPPFQTHFCVVSKPKDCIFPCAFKEVIKKKNMEVEVAATERTLLGFFLAKVHKLDPDILVGHNICGFELEVLLQRINECKVPFWSKIGRLRRSNMPKLGSRSGFGERNATCGRMICDVEISVKELIHCKSYHLSELVQQILKTERIVIPTENIRNMYSEPSHLLYLLEHIWKDARFILQIMCELNVLPLALQITNIAGNIMSRTLMGGRSERNEFLLLHAFYENNYIVPDKQIFRKPQQKPGDEDEEIDGDTNKYKKGRKKAAYAGGLVLDPKVGFYDKFILLLDFNSLYPSIIQEFNICFTTVQRVASETLKATEDEEQEQIPELPDPNLDMGILPREIRKLVERRKQVKQLMKQQDLNPDLVLQYDIRQKALKLTANSMYGCLGFSYSRFYAKPLAALVTYKGREILMHTKEMVQKMNLEVIYGDTDSIMINTNSTNLEEVFKLGNKVKNEVNKLYKLLEIDIDGVFKSLLLLKKKKYAALVVEPTSDGNYITKQELKGLDIVRRDWCDLAKDTGNFVIGQILSDQSRDTIVENIQKRLIEIGENVLNGSVPVSQFEINKALTKDPQDYPDKKSLPHVHVALWINSQGGRKVKAGDTVSYVICQDGSNLPATQRAYAPEQLQKQDNLAIDTQYYLAQQIHPVVARICEPIDGIDAVLIALWLGLDSTQFRVHQYHKDEENDALLGGPAQLTDEEKYKDCEKFKCLCPSCGTENIYDNVFEGSGMDMEPSLNRCSNIDCKASPATFMVQLSNKLIMDIRRCIKKYYDGWLICEEPTCRNRIRRLPLHFSRNGPLCPACMKAVLRPEYSDKSLYTQLCFYRYIFDADCALEKLPEHEKDKLKKQFFTPRVLQDYRKVKNIAEHFLSWSGYSEVNLSKLFANYAGKN

Target ORF information:

RefSeq Version	NM_053479.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DNA polymerase alpha 1, catalytic subunit (Pola1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053479.1

ORF Insert Sequence:

ATGGCGCCTG TTCACGGAGA CGACTGCAAA CTGGAAACAA GCGCTGTGTC AGATTCAGGG 
AGTTTTGTAG CTTCTCGAGC ACGTCGAGAA AAAAAATCAA AGAAAGGACG CCAAGAAGCT 
TTAGAGCGAC TGAAAAAGGC TAAAGCTGGT GAGAAGTACA AGTATGAAGT TGAGGACCTC 
ACAAGTGTTT ATGAAGAAGT TGATGAAGAG CAGTATTCAA AACTGGTTCA GGCACGTCAG 
GATGATGACT GGATCGTGGA TGACGACGGT ATAGGCTATG TGGAAGACGG TAGAGAGATT 
TTTGATGACG ACCTTGAAGA TGATGCACTT GACACCTGTG GGGAAGGAAG TGATGGCAAA 
GCACACAGAA AGGACAGGAA GGATGTAAAG AAACCTTCAG TGACAAAACC AAACAATATC 
AAGGCAATGT TCATTGCTAG TGCTGGGAAG AAAACCACAG ATAAAACAGT TGACTTGTCC 
AAGGATGATC TGCTAGGTGA CATTCTACAG GATCTGAACA CTGAGACACC TCAAATAGCT 
CCACCGCCAG TATTGATACC AAAGAAAAAA AGATCTACTG GAGCTTCACC GAATCCTTTC 
TCTGTGCACA CCGCCACGGC AGTTCCTTCA GGAAAAATTG CTTCCCCTGT CTCCAGAAAG 
GAGCCTCCAT TAACTCCTGT TCCTCTTAAA CGTGCTGAAT TTGCTGGCGA TCTGGCTCAG 
CCAGAGTGTC CAGAAGATGA GCAGGAGTCA GGGGTTATAG AGTTTGAAGA TGGCGACTTT 
GATGAGCCCA TGGACACAGA GGAAGTAGAT GAGGAGGAGC CTGTGACTGC CAAGATTTGG 
GACCAAGAGA GTGAGCCAGT GGAAGGGGTG AAGCATGAGG CAGATCCTGA GACGGGAACC 
ACATCTTTCT TAGACAGTTT TCTCCCAGAT GTTTCTTGTT GGGACATTGA TCAGAAGGAC 
GAAAACAGTT TCTTACTTCA AGAAGTTCAA GTGGACTCCA ATCATCTCCC GTTGGTAAAA 
GGGGCAGATG ATGAACAAGT CTTTCAGTTT TATTGGCTGG ATGCTTATGA AGATCCATAC 
AACCAACCAG GTGTGGTTTT TCTGTTTGGC AAAGTATGGG TCGAATCAGC CAAAACCCAT 
GTGAGCTGTT GTGTTATGGT GAAAAATATT GAGCGAACAC TCTACTTCCT TCCCCGTGAA 
ATGAAAATTG ACCTAAACAC AGGGAAAGAA ACAGCAACTC CAATTACAAT GAAGGATGTT 
TATGAAGAAT TTGATTCAAA AATTTCAGCA AAATATAAAA TAATGAAGTT CAAGTCCAAG 
ATAGTGGAAA AGAACTATGC TTTTGAGATA CCTGATGTTC CAGAAAAATC TGAGTACTTG 
GAAGTTAGAT ACTCGGCAGA AGTGCCACAG CTTCCACAGA ATTTAAAAGG AGAAACATTT 
TCTCATGTAT TTGGAACTAA CACGTCTAGC TTGGAACTGT TCTTGATGAA TAGAAAGATC 
AAAGGACCCT GTTGGCTTGA AGTAAAAAAT CCACAACTCT TGAATCAACC AATCAGTTGG 
TGTAAATTTG AGGCAATGGC TTTGAAACCA GACTTGGTGA ATGTAATTAA GGATGTCAGT 
CCTCCTCCAC TTGTGGTGAT GTCTTTCAGC ATGAAGACAA TGCAAAATGT ACAGAACCAT 
CAACACGAGA TCATTGCTAT GGCAGCGCTG GTTCATCACA ATTTCCCATT AGATAAAGCA 
CCTCCAAAAC CTCCCTTTCA GACGCATTTC TGTGTTGTGT CTAAACCAAA GGACTGTATT 
TTCCCATGCG CTTTCAAAGA AGTCATTAAG AAAAAGAATA TGGAGGTTGA GGTTGCTGCA 
ACAGAAAGAA CTTTGCTAGG ATTTTTCCTT GCAAAAGTTC ACAAACTTGA CCCTGATATT 
CTCGTGGGTC ATAATATTTG TGGTTTTGAA CTAGAAGTGC TACTGCAAAG AATTAATGAG 
TGCAAGGTTC CTTTCTGGTC CAAGATAGGT CGGCTGAGGA GATCCAACAT GCCAAAACTT 
GGGAGCCGGA GTGGATTTGG TGAAAGAAAT GCTACCTGTG GTCGAATGAT ATGTGATGTG 
GAAATTTCAG TAAAAGAATT GATTCATTGT AAAAGTTACC ATTTGTCTGA ACTTGTTCAG 
CAGATTCTGA AAACTGAGAG GATTGTAATT CCAACAGAAA ATATACGAAA CATGTATAGT 
GAACCTTCTC ATCTGTTGTA CCTGTTGGAA CACATCTGGA AAGATGCCAG ATTCATTTTG 
CAGATCATGT GTGAGCTAAA TGTTCTTCCA TTAGCATTGC AGATCACTAA CATCGCTGGG 
AACATTATGT CTAGGACACT GATGGGTGGA CGATCTGAGC GAAACGAGTT TTTGTTGCTT 
CATGCATTTT ATGAAAACAA CTATATTGTG CCTGACAAGC AGATTTTCAG AAAACCTCAG 
CAAAAACCGG GAGATGAAGA TGAAGAAATT GATGGGGATA CCAATAAATA TAAGAAAGGA 
CGCAAGAAAG CAGCTTATGC TGGAGGCTTG GTTTTGGACC CCAAAGTTGG TTTTTATGAC 
AAGTTCATTT TGCTCCTGGA TTTCAACAGT TTATATCCTT CCATCATTCA GGAATTTAAC 
ATTTGTTTTA CAACAGTGCA AAGAGTTGCT TCAGAGACAC TGAAAGCTAC AGAGGATGAG 
GAACAAGAAC AGATTCCAGA GTTGCCAGAT CCAAACTTAG ATATGGGCAT TTTGCCCAGA 
GAGATCCGGA AACTGGTAGA GCGGAGAAAA CAAGTGAAAC AGCTAATGAA ACAGCAAGAT 
TTAAATCCAG ACCTTGTTCT TCAGTATGAT ATCCGACAGA AGGCTTTGAA GCTCACAGCG 
AACAGTATGT ATGGTTGCCT GGGATTTTCG TACAGCAGAT TTTATGCTAA ACCGCTGGCT 
GCCTTGGTGA CATACAAAGG AAGGGAGATT TTGATGCACA CGAAAGAGAT GGTACAGAAG 
ATGAATCTTG AAGTTATTTA TGGAGACACA GATTCAATTA TGATAAATAC CAATAGCACC 
AACCTGGAAG AAGTCTTTAA GTTGGGAAAC AAGGTAAAAA ATGAAGTGAA TAAATTGTAC 
AAACTGCTTG AAATAGACAT TGATGGTGTT TTCAAGTCTC TGCTATTGCT GAAGAAAAAG 
AAATATGCTG CTCTAGTTGT CGAACCAACA TCCGATGGGA ATTACATCAC CAAGCAGGAA 
CTGAAAGGAT TGGATATAGT TAGAAGGGAC TGGTGTGATC TTGCCAAGGA CACCGGAAAC 
TTTGTCATTG GCCAGATTCT TTCTGATCAA AGTCGGGACA CCATTGTGGA AAACATTCAG 
AAGAGGTTAA TAGAAATTGG AGAAAATGTG CTAAACGGCA GTGTCCCAGT GAGCCAGTTT 
GAAATTAACA AGGCATTGAC AAAGGATCCC CAGGATTACC CTGATAAAAA AAGTCTACCT 
CATGTACATG TTGCACTGTG GATAAATTCT CAAGGAGGCA GGAAGGTGAA AGCTGGCGAT 
ACAGTGTCAT ATGTTATCTG TCAGGATGGG TCAAACCTCC CTGCAACTCA GAGGGCCTAT 
GCACCCGAGC AATTGCAGAA ACAGGACAAT TTAGCCATCG ACACCCAGTA CTACTTGGCC 
CAGCAGATCC ACCCTGTTGT GGCTCGAATC TGTGAGCCAA TAGATGGAAT TGATGCTGTA 
CTCATTGCAT TATGGCTAGG ACTTGACTCC ACTCAATTTA GAGTTCATCA GTATCACAAA 
GATGAAGAGA ATGATGCTCT CCTTGGTGGT CCAGCACAGC TAACTGATGA AGAGAAATAC 
AAGGATTGTG AAAAATTCAA ATGCCTTTGT CCTTCATGTG GAACTGAAAA TATTTATGAT 
AATGTCTTTG AAGGCTCGGG AATGGATATG GAGCCCAGCT TGAATCGTTG CAGTAATATT 
GATTGTAAGG CTTCACCAGC GACCTTTATG GTACAGTTGA GCAACAAATT GATCATGGAC 
ATTAGACGTT GCATTAAAAA GTACTATGAT GGCTGGTTGA TATGTGAGGA GCCAACTTGT 
CGCAATCGCA TTCGTCGTCT TCCCCTTCAT TTCTCCCGAA ATGGGCCTCT GTGTCCAGCC 
TGCATGAAAG CTGTGCTCAG ACCAGAGTAT TCTGACAAGT CCCTGTACAC CCAGCTGTGC 
TTTTACCGGT ACATTTTTGA TGCGGACTGT GCACTGGAAA AACTTCCTGA ACACGAGAAA 
GATAAATTGA AGAAGCAGTT TTTCACTCCC AGAGTTCTTC AGGACTACAG AAAAGTCAAG 
AACATAGCAG AACACTTCTT GTCCTGGAGT GGCTACTCTG AAGTAAACCT CAGCAAGCTC 
TTCGCCAATT ATGCTGGGAA GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

DNA polymerase ? and ? switch by sharing accessory subunits of DNA polymerase ?.
The Journal of biological chemistry287(21)17281-7(2012 May)
Baranovskiy AG,Lada AG,Siebler HM,Zhang Y,Pavlov YI,Tahirov TH

Distinctive activities of DNA polymerases during human DNA replication.
The FEBS journal273(13)2984-3001(2006 Jul)
Rytk?nen AK,Vaara M,Nethanel T,Kaufmann G,Sormunen R,L??r? E,Nasheuer HP,Rahmeh A,Lee MY,Syv?oja JE,Pospiech H

Transcription factor YB-1 mediates DNA polymerase alpha gene expression.
The Journal of biological chemistry280(9)7702-11(2005 Mar)
En-Nia A,Yilmaz E,Klinge U,Lovett DH,Stefanidis I,Mertens PR

The role of DNA polymerase activity in human non-homologous end joining.
Nucleic acids research29(15)3277-88(2001 Aug)
Pospiech H,Rytk?nen AK,Syv?oja JE

A mutation in subunit B of the DNA polymerase alpha-primase complex from Novikoff hepatoma cells concomitant with a conformational change and abnormal catalytic properties of the DNA polymerase alpha-primase complex.
Molecular carcinogenesis31(4)171-83(2001 Aug)
Popanda O,Flohr C,Dai JC,Hunzicker A,Thielmann HW

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Pola1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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