pola1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	pola1
Entrez Gene ID	317740
Full Name	polymerase (DNA directed), alpha 1
Synonyms	cb678,pola
General protein information	Preferred Names polymerase (DNA directed), alpha 1 Names DNA polymerase alpha catalytic subunit
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001305464.1	NP_001292393.1	DNA polymerase alpha catalytic subunit

Pathways from BioSystems

Homologies

	POLA1	XP_001091195.1
Gallus gallus (chicken)	POLA1	XP_416792.3
Eremothecium gossypii	AGOS_AER381C	NP_985237.1
Homo sapiens (human)	POLA1	NP_058633.2
Rattus norvegicus (Norway rat)	Pola1	NP_445931.1
Schizosaccharomyces pombe (fission yeast)	pol1	NP_594186.1
Pan troglodytes (chimpanzee)	POLA1	XP_003317445.2
Mus musculus (house mouse)	Pola1	NP_032918.1
Danio rerio (zebrafish)	pola1	XP_005162809.1
Drosophila melanogaster (fruit fly)	DNApol-alpha180	NP_536736.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001929	XP_321134.5
Caenorhabditis elegans (roundworm)	Y47D3A.29	NP_001255109.1
Magnaporthe oryzae (rice blast fungus)	MGG_06397	XP_003717174.1
Neurospora crassa	NCU07870	XP_963131.2
Arabidopsis thaliana (thale cress)	ICU2	NP_201511.2
Xenopus tropicalis (tropical clawed frog)	pola1	XP_004911812.1
Bos taurus (cattle)	POLA1	NP_001192994.1
Saccharomyces cerevisiae (baker's yeast)	POL1	NP_014297.3
Kluyveromyces lactis	KLLA0C13255g	XP_452791.1
Canis lupus familiaris (dog)	POLA1	XP_005641271.1
Oryza sativa (rice)	Os01g0868300	NP_001044920.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Telomerase Is Essential for Zebrafish Heart Regeneration.
Bednarek D, Gonz?lez-Rosa JM, Guzm?n-Mart?nez G, Guti?rrez-Guti?rrez ?, Aguado T, S?nchez-Ferrer C, Marques IJ, Galardi-Castilla M, de Diego I, G?mez MJ, Cort?s A, Zapata A, Jim?nez-Borreguero LJ, Mercader N, Flores I
Cell reports12(10)1691-703(2015 Sep)

DNA hypomethylation induces a DNA replication-associated cell cycle arrest to block hepatic outgrowth in uhrf1 mutant zebrafish embryos.
Jacob V, Chernyavskaya Y, Chen X, Tan PS, Kent B, Hoshida Y, Sadler KC
Development (Cambridge, England)142(3)510-21(2015 Feb)

Zebrafish midbrain slow-amplifying progenitors exhibit high levels of transcripts for nucleotide and ribosome biogenesis.
Recher G, Jouralet J, Brombin A, Heuz? A, Mugniery E, Hermel JM, Desnoulez S, Savy T, Herbomel P, Bourrat F, Peyri?ras N, Jamen F, Joly JS
Development (Cambridge, England)140(24)4860-9(2013 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following pola1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pola1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa28617	NM_001305464.1 Latest version!	Danio rerio polymerase (DNA directed), alpha 1 (pola1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pola1 ( NM_001305464.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001292393.1 Danio rerio polymerase (DNA directed), alpha 1 (pola1), mRNA.

Price & Availability↑

CloneID	ODa28617
Clone ID Related Accession (Same CDS sequence)	NM_001305464.1
Accession Version	NM_001305464.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4407bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	DNA polymerase alpha catalytic subunit
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX470095.12. On Mar 15, 2015 this sequence version replaced XM_001922019.4. Sequence Note: This RefSeq record was created from genomic sequence data because no single transcript from the same strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. ##Evidence-Data-START## Transcript exon combination :: GFIL01030868.1, GDQH01001399.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTCCTG TTTCAAATCC CGATAAAGAC ATGGACGGCG GAGATTGTGA TGCATCCGAC 
ACATGTGGCA TTGCCGCCTC ACGTTCCAGA AGGGAGAAAA AAGAGAAAGT CGGGCGCAAG 
TCTGCTTTAG AGCAACTTAA AAGAGCCAAG AAAGGAGAGA AAGTAAAATA CGAGGTGGAG 
GAGTTCACCA GCATTTATGA TGAAGTGGAT GAAGAGCAGT ACTCGAAAAT AGTTCGGGAG 
AGACAGGATG ATGATTGGAT CATTGATGAC GATGGGACAG GTTATGTGGA GGATGGCAGA 
GAAATCTTTG ATGAAGAATT GGATGATGAT GCATTGGGCC CTAAAAGTAA ACAGGCTGCA 
AAAGGAGGTG ATTCGAAAAA GAATGTGAAG AAATCATCTG TCAGTAAACC CAACAGCATT 
AAAAGCATGT TTATGAACAG TAATGTAAAG AAACCTGCGG AGAAACATGT GGATTTGTCT 
CAAGATGATC TGCTGGGAGA TATTTTGCAG GACTTGCACT CTGAGAAATC AGTTGTGCTC 
ACTCCACCAC CGATCATTAC TCTGAAGAAG AAGAAAACAC TGGGCTCCCC TATGAATCCA 
TTTTCAGTCA AACCACAGAT GCCAAAGGAG TTGCCTGAAG TCCACGTATC AAAGCCGAGG 
CCTGTGGTGA CACGAGTTCC TCCTGCTGAC CCTTCGCCCA GGTCTGCAGC TTCTGTTGAA 
CCTAGTAAAG CAACTGCCGC ACAGATAAAG AAACCTAAGA TCGAAGAGCC TGAAGAGGAG 
GCAATCGAGT TTGATGAGAT GGACTTTGAT GAGCCCATGG AGGCAGAAAA CCTGGAGGAG 
GAGCCTGTGA AAGAGAAGGC GGAGCCCGAG ACCAAGCCCA TTGTTAAAGT GGAGCCTAAG 
ACTGAACCAC AGGATAAAAT GTTAATATCT GGACTGGTTG GGGGCATGGG CTGGGAGAAG 
ATGGAGGAGG GGAAACCCAT CGAGACTCCG GTGGAAGTGC AGGTGGACTC GAGTCAACTC 
CCGCTGGTGG AGGGACCAGA TGGAGATATG GTCTTCCGCT TCTACTGGTT GGATGCTTTT 
GAAGATCAGT ATAACCAACC TGGAGTGGTG TTCCTGTTTG GAAAAGTTTG GATCGAGTCT 
GCTGAAGCAC ATGTTAGCTG TTGTGTTGCT GTAAAGAACA TAGAGAGAAC GATGTATCTC 
CTTCCACGAG AACATAAAGT AAATCTGGCA ACCGGCGAGA AGGGCGTTGC AGTAGGAATG 
ATGGATGTGT ACAAGGAGTT CAATGAGATC TCTGAGAAGT TCAAAATTAT GAAGTTTAAA 
TCCAAGCCGG TGACCAAGAA TTATGCTTTT GAGATCCCAG ATATCCCCTC TCAGAGCGAG 
TACCTAGAAG TCAAATACTC TGCTGAGATT CCTTCATTGC CATCTGACCT GAAGGGCACT 
ACATTTTCCC ATGTGTTTGG TACCAACACC TCCAGTTTGG AACACTTCCT TCTGAATAGG 
AAGATTCGTG GGCCTTGCTG GCTTGATGTG ACAACCCCTC AGTTAAGCAA TCAGCCTATT 
AGCTGGTGTA AAGTGGAGGC CGTTGCACAG AAGAGTGACT TGATCTCAGT AGTAAAAGAT 
CTGTCGCCTC CTCCACTGGT GGTCATGTCC ATAAGCCTCA AGACCGTGCA GAACCCCAAG 
ACTCACCAGA ATGAGATTGT TTCCATCGCT GCCCTCGTTC ATCATACATT TCCTCTGGAC 
AAAGCTCCTC CACGGCCGCC GTTTCAGACT CATTTCTGTG TGGTGAGCAA GCCTAATGAC 
TGTGTCTTCC CTTATGACTT TAAGGATGCA GTTAGGAAAA AGAACGGCAT TGTCGAGATT 
GCTGGTACAG AACGTGCGCT TTTAGGCTTC TTCTTGGCCA AGATGCACAA GATCGATCCA 
GATGTCTTAG TGGGCCATGA CATCTTTGGT TTTGACCTTG AAGTGCTTTT GCAGAGGATC 
AATGTGTGTA AAGTACCTCA CTGGTCAAAG ATCGGCCGTC TAAGAAGAGC CAACATGCCC 
AAACTGGGGG GTCGCAGTGG CTTTGCTGAG AAGAGCGCAA CTTGTGGGCG CCTGGTCTGT 
GATATTGAAA TTTCAGCCAA AGAGCTGATC CGGTGCAAGA GTTATAATCT GACTGAGCTG 
GTGTCCCAGA TCTTGAAGGC GGAGAGGCCG GTGGTGCCAC CAGAGAACAT CAGGAATTTC 
TACAGTGATT CCACACATTT ACTCTACCTT TTGGAGCTCA CCTGGATGGA TGCTAAGCTC 
ATTCTACAAA TCATGTGCGA ATTAAATGTA CTCCCGCTAG CACTGCAGAT CACTAACATC 
GCTGGTAATG TCATGTCTCG TACTTTGATG GGTGGACGCT CTGAGAGGAA TGAATATCTT 
CTGCTCCATG CTTTCCATGA GAGGGACTTC ATTGTTCCAG ACAAGCAGTT CTTCAAGAAG 
GCACAACAAG ATCTGGGTGA GGATGAAGAG GGTGACTCTG GAAAGGGCAA GTCAAACAAA 
ACTAGGAAGA AGGCAGCATA TGCTGGAGGT CTGGTGCTCG ACCCAAAAGT CGGTTTCTAT 
GACAAGTTCA TTCTGCTGTT GGACTTCAAT AGTTTGTACC CCTCCATCAT TCAAGAGTTC 
AACATCTGCT TTACAACCGT TGAGAGAGGA GCTACCAACA CACGCAAGAA AACTGAGGAG 
GATGAAGATG AGATTCCTGA GCTTCCGGAT CAGAGTCTGG AAATGGGCAT TCTTCCCAAG 
GAAATCCGCA AACTGGTGGA GCGCAGACGA CAAGTCAAAC AGCTAATGAA AGAGCCAGAC 
CTCAACCCGG ATCTGTACTT GCAGTATGAC ATCAGGCAAA AGGCCCTGAA GCTGACAGCT 
AACAGCATGT ACGGATGCCT TGGCTTTTCC TTCAGTCGCT TTTACGCCAA GCCTTTGGCA 
GCATTGGTCA CGCATAAAGG AAGAGAGATT TTGATGCACA CCAAAGAAAT GGTACAGAGG 
ATGAATCTGG AGGTCATATA CGGGGACACT GACTCCATCA TGATTAACAC CAACAGCACC 
AATTTAGAGG AGGTCTTCAA ACTGGGAAAC AAGGTCAAAA GTGAGGTGAA TAAACTCTAT 
AAGCTCCTGG AGATAGACAT AGATGGTGTA TTTAAGTCCC TTCTGCTGCT AAAAAAGAAG 
AAATATGCAG CACTGATGGT TGAGCCACAG GGAGATGGCA AATACACCAC CAAGCAGGAG 
CTCAAAGGCC TGGACATTGT GCGTAGAGAT TGGTGCGACC TCGCCAAGGA GTGTGGAAAT 
TATGTTATTG GTCAGATTCT GTCAGACCAG AATCGGGACA CCATCATAGA GAACATTCAG 
AAACATCTGA TTGAAATCGG AGAGAAGGTG GCCAACGGCA ACATCCCACT CAACATGTTT 
GAGATACACA AGTCTCTGAC AAAGGAACCT CAGGACTACC CTGACAAGAA GAGTCTCCCT 
CATGTCCATG TTGCTCTCTG GATAAACTCC CAGGGTGGGA AGAAGGTTAA AGCGGGTGAC 
ACTGTTTCTT ATGTCATCTG TCAGGATGGT TCAAACCTGG CTGCCAGTCA GAGGGCATAT 
ACCTTGGAAC AGCTACAAAA ACAGCCAGGA CTCAATCTGG ACACACAGTA CTACCTGTCC 
CAGCAGGTGC ACCCTGTGGT CGGCCGAATC TGTGAGCCTA TTGAAGGCAT TGATGCAGTG 
CTTATTGCTA CGTGGTTAGG ACTTGACCCA AGTCACTTCC GAGCCCAACA GCAGCAGCGC 
GAGGACGAAG CAGACGATTT CCTAGGTGCT CCAGCCCAGC TCACAGACGA AGAGCGTTAC 
CGAGATTGCG AGAGGTTTAC CTTCACCTGC CCTGACTGTG GAACCGAAAA CATCTACGAC 
AACGTGTTTG AAGGGACGGG GAGCACCTTG CAGCTTAGCC TGAAGAGTTG CTGCCACGTT 
CCCTGCGGGG GCAGTCCGAT GGACCATCAA GTACAAATTG GCAATAAACT GCAGTTGGAC 
ATTCGCCGCC ACATCAGGAG ATACTACAGC GGATGGCTGC TATGTGAGGA TCAGGCCTGC 
CGGAACCGAA TCCGTCGGCT GCCCATAGCA TTCTCCAGGT CGGGACCGAT CTGTCCAGCC 
TGTCTTAGAT CCACACTGAA GCCTGAGTAT TCTGAGAAGG CCCTCTACAA CCAACTGAGC 
TTCTACAGAT ACATCTTTGA CTGGGAATAT GCCATCAACA AGGTCCTGAC AGGAGATGAT 
AAATTAAAAT TCAAGAGTAA AGAATGGAAA GAAGAAATGG AAGTGTACAA AAAGCTGAAG 
GGTGTAACAG ACAACATCCT CGCCACCAGC GGATACTCTG AAGTCAACCT TGCCAAGCTT 
TTCCAGGCCT TTATAACCCT CAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001292393.1
CDS	132..4538
Translation	MAPVSNPDKDMDGGDCDASDTCGIAASRSRREKKEKVGRKSALEQLKRAKKGEKVKYEVEEFTSIYDEVDEEQYSKIVRERQDDDWIIDDDGTGYVEDGREIFDEELDDDALGPKSKQAAKGGDSKKNVKKSSVSKPNSIKSMFMNSNVKKPAEKHVDLSQDDLLGDILQDLHSEKSVVLTPPPIITLKKKKTLGSPMNPFSVKPQMPKELPEVHVSKPRPVVTRVPPADPSPRSAASVEPSKATAAQIKKPKIEEPEEEAIEFDEMDFDEPMEAENLEEEPVKEKAEPETKPIVKVEPKTEPQDKMLISGLVGGMGWEKMEEGKPIETPVEVQVDSSQLPLVEGPDGDMVFRFYWLDAFEDQYNQPGVVFLFGKVWIESAEAHVSCCVAVKNIERTMYLLPREHKVNLATGEKGVAVGMMDVYKEFNEISEKFKIMKFKSKPVTKNYAFEIPDIPSQSEYLEVKYSAEIPSLPSDLKGTTFSHVFGTNTSSLEHFLLNRKIRGPCWLDVTTPQLSNQPISWCKVEAVAQKSDLISVVKDLSPPPLVVMSISLKTVQNPKTHQNEIVSIAALVHHTFPLDKAPPRPPFQTHFCVVSKPNDCVFPYDFKDAVRKKNGIVEIAGTERALLGFFLAKMHKIDPDVLVGHDIFGFDLEVLLQRINVCKVPHWSKIGRLRRANMPKLGGRSGFAEKSATCGRLVCDIEISAKELIRCKSYNLTELVSQILKAERPVVPPENIRNFYSDSTHLLYLLELTWMDAKLILQIMCELNVLPLALQITNIAGNVMSRTLMGGRSERNEYLLLHAFHERDFIVPDKQFFKKAQQDLGEDEEGDSGKGKSNKTRKKAAYAGGLVLDPKVGFYDKFILLLDFNSLYPSIIQEFNICFTTVERGATNTRKKTEEDEDEIPELPDQSLEMGILPKEIRKLVERRRQVKQLMKEPDLNPDLYLQYDIRQKALKLTANSMYGCLGFSFSRFYAKPLAALVTHKGREILMHTKEMVQRMNLEVIYGDTDSIMINTNSTNLEEVFKLGNKVKSEVNKLYKLLEIDIDGVFKSLLLLKKKKYAALMVEPQGDGKYTTKQELKGLDIVRRDWCDLAKECGNYVIGQILSDQNRDTIIENIQKHLIEIGEKVANGNIPLNMFEIHKSLTKEPQDYPDKKSLPHVHVALWINSQGGKKVKAGDTVSYVICQDGSNLAASQRAYTLEQLQKQPGLNLDTQYYLSQQVHPVVGRICEPIEGIDAVLIATWLGLDPSHFRAQQQQREDEADDFLGAPAQLTDEERYRDCERFTFTCPDCGTENIYDNVFEGTGSTLQLSLKSCCHVPCGGSPMDHQVQIGNKLQLDIRRHIRRYYSGWLLCEDQACRNRIRRLPIAFSRSGPICPACLRSTLKPEYSEKALYNQLSFYRYIFDWEYAINKVLTGDDKLKFKSKEWKEEMEVYKKLKGVTDNILATSGYSEVNLAKLFQAFITLK

Target ORF information:

RefSeq Version	NM_001305464.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio polymerase (DNA directed), alpha 1 (pola1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001305464.1

ORF Insert Sequence:

ATGGCTCCTG TTTCAAATCC CGATAAAGAC ATGGACGGCG GAGATTGTGA TGCATCCGAC 
ACATGTGGCA TTGCCGCCTC ACGTTCCAGA AGGGAGAAAA AAGAGAAAGT CGGGCGCAAG 
TCTGCTTTAG AGCAACTTAA AAGAGCCAAG AAAGGAGAGA AAGTAAAATA CGAGGTGGAG 
GAGTTCACCA GCATTTATGA TGAAGTGGAT GAAGAGCAGT ACTCGAAAAT AGTTCGGGAG 
AGACAGGATG ATGATTGGAT CATTGATGAC GATGGGACAG GTTATGTGGA GGATGGCAGA 
GAAATCTTTG ATGAAGAATT GGATGATGAT GCATTGGGCC CTAAAAGTAA ACAGGCTGCA 
AAAGGAGGTG ATTCGAAAAA GAATGTGAAG AAATCATCTG TCAGTAAACC CAACAGCATT 
AAAAGCATGT TTATGAACAG TAATGTAAAG AAACCTGCGG AGAAACATGT GGATTTGTCT 
CAAGATGATC TGCTGGGAGA TATTTTGCAG GACTTGCACT CTGAGAAATC AGTTGTGCTC 
ACTCCACCAC CGATCATTAC TCTGAAGAAG AAGAAAACAC TGGGCTCCCC TATGAATCCA 
TTTTCAGTCA AACCACAGAT GCCAAAGGAG TTGCCTGAAG TCCACGTATC AAAGCCGAGG 
CCTGTGGTGA CACGAGTTCC TCCTGCTGAC CCTTCGCCCA GGTCTGCAGC TTCTGTTGAA 
CCTAGTAAAG CAACTGCCGC ACAGATAAAG AAACCTAAGA TCGAAGAGCC TGAAGAGGAG 
GCAATCGAGT TTGATGAGAT GGACTTTGAT GAGCCCATGG AGGCAGAAAA CCTGGAGGAG 
GAGCCTGTGA AAGAGAAGGC GGAGCCCGAG ACCAAGCCCA TTGTTAAAGT GGAGCCTAAG 
ACTGAACCAC AGGATAAAAT GTTAATATCT GGACTGGTTG GGGGCATGGG CTGGGAGAAG 
ATGGAGGAGG GGAAACCCAT CGAGACTCCG GTGGAAGTGC AGGTGGACTC GAGTCAACTC 
CCGCTGGTGG AGGGACCAGA TGGAGATATG GTCTTCCGCT TCTACTGGTT GGATGCTTTT 
GAAGATCAGT ATAACCAACC TGGAGTGGTG TTCCTGTTTG GAAAAGTTTG GATCGAGTCT 
GCTGAAGCAC ATGTTAGCTG TTGTGTTGCT GTAAAGAACA TAGAGAGAAC GATGTATCTC 
CTTCCACGAG AACATAAAGT AAATCTGGCA ACCGGCGAGA AGGGCGTTGC AGTAGGAATG 
ATGGATGTGT ACAAGGAGTT CAATGAGATC TCTGAGAAGT TCAAAATTAT GAAGTTTAAA 
TCCAAGCCGG TGACCAAGAA TTATGCTTTT GAGATCCCAG ATATCCCCTC TCAGAGCGAG 
TACCTAGAAG TCAAATACTC TGCTGAGATT CCTTCATTGC CATCTGACCT GAAGGGCACT 
ACATTTTCCC ATGTGTTTGG TACCAACACC TCCAGTTTGG AACACTTCCT TCTGAATAGG 
AAGATTCGTG GGCCTTGCTG GCTTGATGTG ACAACCCCTC AGTTAAGCAA TCAGCCTATT 
AGCTGGTGTA AAGTGGAGGC CGTTGCACAG AAGAGTGACT TGATCTCAGT AGTAAAAGAT 
CTGTCGCCTC CTCCACTGGT GGTCATGTCC ATAAGCCTCA AGACCGTGCA GAACCCCAAG 
ACTCACCAGA ATGAGATTGT TTCCATCGCT GCCCTCGTTC ATCATACATT TCCTCTGGAC 
AAAGCTCCTC CACGGCCGCC GTTTCAGACT CATTTCTGTG TGGTGAGCAA GCCTAATGAC 
TGTGTCTTCC CTTATGACTT TAAGGATGCA GTTAGGAAAA AGAACGGCAT TGTCGAGATT 
GCTGGTACAG AACGTGCGCT TTTAGGCTTC TTCTTGGCCA AGATGCACAA GATCGATCCA 
GATGTCTTAG TGGGCCATGA CATCTTTGGT TTTGACCTTG AAGTGCTTTT GCAGAGGATC 
AATGTGTGTA AAGTACCTCA CTGGTCAAAG ATCGGCCGTC TAAGAAGAGC CAACATGCCC 
AAACTGGGGG GTCGCAGTGG CTTTGCTGAG AAGAGCGCAA CTTGTGGGCG CCTGGTCTGT 
GATATTGAAA TTTCAGCCAA AGAGCTGATC CGGTGCAAGA GTTATAATCT GACTGAGCTG 
GTGTCCCAGA TCTTGAAGGC GGAGAGGCCG GTGGTGCCAC CAGAGAACAT CAGGAATTTC 
TACAGTGATT CCACACATTT ACTCTACCTT TTGGAGCTCA CCTGGATGGA TGCTAAGCTC 
ATTCTACAAA TCATGTGCGA ATTAAATGTA CTCCCGCTAG CACTGCAGAT CACTAACATC 
GCTGGTAATG TCATGTCTCG TACTTTGATG GGTGGACGCT CTGAGAGGAA TGAATATCTT 
CTGCTCCATG CTTTCCATGA GAGGGACTTC ATTGTTCCAG ACAAGCAGTT CTTCAAGAAG 
GCACAACAAG ATCTGGGTGA GGATGAAGAG GGTGACTCTG GAAAGGGCAA GTCAAACAAA 
ACTAGGAAGA AGGCAGCATA TGCTGGAGGT CTGGTGCTCG ACCCAAAAGT CGGTTTCTAT 
GACAAGTTCA TTCTGCTGTT GGACTTCAAT AGTTTGTACC CCTCCATCAT TCAAGAGTTC 
AACATCTGCT TTACAACCGT TGAGAGAGGA GCTACCAACA CACGCAAGAA AACTGAGGAG 
GATGAAGATG AGATTCCTGA GCTTCCGGAT CAGAGTCTGG AAATGGGCAT TCTTCCCAAG 
GAAATCCGCA AACTGGTGGA GCGCAGACGA CAAGTCAAAC AGCTAATGAA AGAGCCAGAC 
CTCAACCCGG ATCTGTACTT GCAGTATGAC ATCAGGCAAA AGGCCCTGAA GCTGACAGCT 
AACAGCATGT ACGGATGCCT TGGCTTTTCC TTCAGTCGCT TTTACGCCAA GCCTTTGGCA 
GCATTGGTCA CGCATAAAGG AAGAGAGATT TTGATGCACA CCAAAGAAAT GGTACAGAGG 
ATGAATCTGG AGGTCATATA CGGGGACACT GACTCCATCA TGATTAACAC CAACAGCACC 
AATTTAGAGG AGGTCTTCAA ACTGGGAAAC AAGGTCAAAA GTGAGGTGAA TAAACTCTAT 
AAGCTCCTGG AGATAGACAT AGATGGTGTA TTTAAGTCCC TTCTGCTGCT AAAAAAGAAG 
AAATATGCAG CACTGATGGT TGAGCCACAG GGAGATGGCA AATACACCAC CAAGCAGGAG 
CTCAAAGGCC TGGACATTGT GCGTAGAGAT TGGTGCGACC TCGCCAAGGA GTGTGGAAAT 
TATGTTATTG GTCAGATTCT GTCAGACCAG AATCGGGACA CCATCATAGA GAACATTCAG 
AAACATCTGA TTGAAATCGG AGAGAAGGTG GCCAACGGCA ACATCCCACT CAACATGTTT 
GAGATACACA AGTCTCTGAC AAAGGAACCT CAGGACTACC CTGACAAGAA GAGTCTCCCT 
CATGTCCATG TTGCTCTCTG GATAAACTCC CAGGGTGGGA AGAAGGTTAA AGCGGGTGAC 
ACTGTTTCTT ATGTCATCTG TCAGGATGGT TCAAACCTGG CTGCCAGTCA GAGGGCATAT 
ACCTTGGAAC AGCTACAAAA ACAGCCAGGA CTCAATCTGG ACACACAGTA CTACCTGTCC 
CAGCAGGTGC ACCCTGTGGT CGGCCGAATC TGTGAGCCTA TTGAAGGCAT TGATGCAGTG 
CTTATTGCTA CGTGGTTAGG ACTTGACCCA AGTCACTTCC GAGCCCAACA GCAGCAGCGC 
GAGGACGAAG CAGACGATTT CCTAGGTGCT CCAGCCCAGC TCACAGACGA AGAGCGTTAC 
CGAGATTGCG AGAGGTTTAC CTTCACCTGC CCTGACTGTG GAACCGAAAA CATCTACGAC 
AACGTGTTTG AAGGGACGGG GAGCACCTTG CAGCTTAGCC TGAAGAGTTG CTGCCACGTT 
CCCTGCGGGG GCAGTCCGAT GGACCATCAA GTACAAATTG GCAATAAACT GCAGTTGGAC 
ATTCGCCGCC ACATCAGGAG ATACTACAGC GGATGGCTGC TATGTGAGGA TCAGGCCTGC 
CGGAACCGAA TCCGTCGGCT GCCCATAGCA TTCTCCAGGT CGGGACCGAT CTGTCCAGCC 
TGTCTTAGAT CCACACTGAA GCCTGAGTAT TCTGAGAAGG CCCTCTACAA CCAACTGAGC 
TTCTACAGAT ACATCTTTGA CTGGGAATAT GCCATCAACA AGGTCCTGAC AGGAGATGAT 
AAATTAAAAT TCAAGAGTAA AGAATGGAAA GAAGAAATGG AAGTGTACAA AAAGCTGAAG 
GGTGTAACAG ACAACATCCT CGCCACCAGC GGATACTCTG AAGTCAACCT TGCCAAGCTT 
TTCCAGGCCT TTATAACCCT CAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Telomerase Is Essential for Zebrafish Heart Regeneration.
Cell reports12(10)1691-703(2015 Sep)
Bednarek D,Gonz?lez-Rosa JM,Guzm?n-Mart?nez G,Guti?rrez-Guti?rrez ?,Aguado T,S?nchez-Ferrer C,Marques IJ,Galardi-Castilla M,de Diego I,G?mez MJ,Cort?s A,Zapata A,Jim?nez-Borreguero LJ,Mercader N,Flores I

DNA hypomethylation induces a DNA replication-associated cell cycle arrest to block hepatic outgrowth in uhrf1 mutant zebrafish embryos.
Development (Cambridge, England)142(3)510-21(2015 Feb)
Jacob V,Chernyavskaya Y,Chen X,Tan PS,Kent B,Hoshida Y,Sadler KC

Zebrafish midbrain slow-amplifying progenitors exhibit high levels of transcripts for nucleotide and ribosome biogenesis.
Development (Cambridge, England)140(24)4860-9(2013 Dec)
Recher G,Jouralet J,Brombin A,Heuz? A,Mugniery E,Hermel JM,Desnoulez S,Savy T,Herbomel P,Bourrat F,Peyri?ras N,Jamen F,Joly JS

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pola1 cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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