POLA1 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	POLA1
Entrez Gene ID	418593
Full Name	DNA polymerase alpha 1, catalytic subunit
Synonyms	POLA
General protein information	Preferred Names DNA polymerase alpha 1, catalytic subunit Names DNA polymerase alpha catalytic subunit polymerase (DNA directed), alpha 1, catalytic subunit polymerase (DNA) alpha 1, catalytic subunit polymerase (DNA-directed), alpha
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_025146469.1	XP_025002237.1	DNA polymerase alpha catalytic subunit isoform X1
XM_015302671.2	XP_015158157.1	DNA polymerase alpha catalytic subunit isoform X2
XM_015302675.1	XP_015158161.1	DNA polymerase alpha catalytic subunit isoform X2
XM_015302671.1	XP_015158157.1	DNA polymerase alpha catalytic subunit isoform X1
XM_015302675.2	XP_015158161.1	DNA polymerase alpha catalytic subunit isoform X3

Pathways from BioSystems

Homologies

	POLA1	XP_001091195.1
Gallus gallus (chicken)	POLA1	XP_416792.3
Eremothecium gossypii	AGOS_AER381C	NP_985237.1
Homo sapiens (human)	POLA1	NP_058633.2
Rattus norvegicus (Norway rat)	Pola1	NP_445931.1
Schizosaccharomyces pombe (fission yeast)	pol1	NP_594186.1
Pan troglodytes (chimpanzee)	POLA1	XP_003317445.2
Mus musculus (house mouse)	Pola1	NP_032918.1
Danio rerio (zebrafish)	pola1	XP_005162809.1
Drosophila melanogaster (fruit fly)	DNApol-alpha180	NP_536736.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001929	XP_321134.5
Caenorhabditis elegans (roundworm)	Y47D3A.29	NP_001255109.1
Magnaporthe oryzae (rice blast fungus)	MGG_06397	XP_003717174.1
Neurospora crassa	NCU07870	XP_963131.2
Arabidopsis thaliana (thale cress)	ICU2	NP_201511.2
Xenopus tropicalis (tropical clawed frog)	pola1	XP_004911812.1
Bos taurus (cattle)	POLA1	NP_001192994.1
Saccharomyces cerevisiae (baker's yeast)	POL1	NP_014297.3
Kluyveromyces lactis	KLLA0C13255g	XP_452791.1
Canis lupus familiaris (dog)	POLA1	XP_005641271.1
Oryza sativa (rice)	Os01g0868300	NP_001044920.1

Gene Ontology

The following POLA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POLA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa30439	XM_015302671.2 Latest version!	Gallus gallus DNA polymerase alpha 1, catalytic subunit (POLA1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OGa30440	XM_015302675.2 Latest version!	Gallus gallus DNA polymerase alpha 1, catalytic subunit (POLA1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OGa142989	XM_025146469.1 Latest version!	Gallus gallus DNA polymerase alpha 1, catalytic subunit (POLA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
View Old Accession Versions >>
OGa30439	XM_015302671.1	Gallus gallus polymerase (DNA directed), alpha 1, catalytic subunit (POLA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OGa30440	XM_015302675.1	Gallus gallus polymerase (DNA directed), alpha 1, catalytic subunit (POLA1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

POLA1 ( XM_015302671.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015158157.1 Gallus gallus DNA polymerase alpha 1, catalytic subunit (POLA1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa30439
Clone ID Related Accession (Same CDS sequence)	XM_015302671.2 , XM_015302671.1
Accession Version	XM_015302671.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	DNA polymerase alpha catalytic subunit isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015302671.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAGG ATGCGATGCC TGAGTCTGGG AGCTTTGCAA CGTCACGTTC TAGGCGTGAG 
AGGAAGTCAA AAAGAGGTCA GCAAGAAGCA CTGGAACGTC TGAAAAAGGC CAAAGCTGGG 
GAAAAATTAA AATACGAGGT TGAGGAGTTC ACTGGTGTTT ACGATGAAAT AGATGAAGAA 
CAGTACTCCA AGATAGTCAG AGAACGTCAG GATGATGACT GGATTGTTGA TGATGATGGG 
ATAGGTTATG TAGAAGATGG ACGGGAAATC TTTGATGAAG ATCTAGACGA TGATGCTCTT 
GGTTCCAGTA AGAAAGGAAA AACCGGCAAA ACTTCTACAA TTGGTAAAAA GAATGTGAAA 
AAATCTGTGG TAGCAAAGCC AAACACAATT AAATCTATGT TTATTGCCAG TGCTGGGAAG 
AAAAACACGG ACAAAACTGT GGACCTGTCA AAGGATGATT TACTAGGGGA TATTCTTCAA 
GATCTAAATG CTGAAACTGT TCAGCTAAGT CCACCACCGA TTTTAGTGCT GAAAAGGAAA 
AGATCTGCTG GAATGCCACT GAATCCTTTC TCTGTACAGC GCCAGTCTCC TAAGGTAATC 
ACCCCTGTAT CAAAGAAAGC TCCTGTTCAA CTCAGGAAAG AAGCTCGTCC TCCAGCTTCA 
TTTCATCCTA AACATCCAAA TTACACAGCA AAATCTGTCA CAGCCTCTGA AGAGAATGAG 
GATACCAAGT ATGTGCAGAA AGCTACAGAA AATGGGAGAG TAGTGAAGAC AAAAGAAGAA 
AAAGCTATTG AAGATGATGA TCAGGGGATG ATGGATTTTG ATGATGAGGA CTTTGATGAA 
CCTATGGAAG CAGAGCTAAT GGGAGCTGTA CCTGAGACAG CTGAAACCTT GAAAAGAGAC 
AAGGAAGTTG AAAAGAAAGA GATGGAGCAG CTGGAATCTA AGAAGAAAGA GGCATCTGCC 
TTAAGTCATT CTCTCCCAGA TATCTCATGT TGGGACAAAG TAGAGGAGGA TGAAGCTGAT 
CTGGTGGCGA CAGATGTTCA GTTGGACCCC AATCTCCTTC CACTTGTGAA TGGGGAAAAT 
GATGATCAAG TCTTAAGATT TTATTGGCTG GATGCTTATG AAGATCAGTA CAGTCAGCCA 
GGTGTGGTAT TTTTGTTTGG CAAAGTTTGG ATTGAATCGG CGAACACCTA TGTCAGCTGT 
TGTGTGACGG TGAAAAACAT TGAGAGAACT GTTTATCTAC TGCCACGTGA AACAGAGATG 
GATCTATCCA GTGGGAAAGA GACAGAGACT CCAGTAACCA TAATGAATGT TTTCAAGGAA 
TTTAATGACT GCATTTCACC AAAGTATAAG ATTATGAAGT TCAAATCCAA GAAAGTGGAA 
AAATACTATG CGTTTGAGAT CCCGGATGTT CCAGCCAAGT CTGAATACTT GGAAGTTAAA 
TATTCAGCTG AATACCCTCG GCTTCCTCAA GACCTGAAAG GAGAAACGTT TTCACACGCG 
TTTGGAACTA ACACTTCTAG CTTGGAGCTT CTCTTGATGA GTAGAAAGAT CAAAGGACCG 
TGCTGGCTGG AAATCAAAAA TCCACAGCCC TCAAATCAGT CAGTCAGCTG GTGTAAGGTA 
GAGGCAGTAG CCATGAAGCC TGGCTTGGTG AATGTGGTTA AAGATCTTCC TCCTCCTCCT 
CCTCTTGTGG TAATGTCCTT CAGCATGAAG ACCATTCAGA ACCCAAAGAC TCACCAGAAT 
GAGATTGTGG CTATGGCAGC TCTGATTCAT CAGAAATTTC CTTTGGATAA AGCTCCACCT 
CAGCCTCCTT TTCAGACACA CTTCTGTGCT GTTTCCAAAC CGAATGATTG CATTTTTCCT 
TATGACTTCA AAGAAGAGAT TAAAAAGAAG AATGCTAAAA TAGAAATTGC TGCAACAGAG 
AGAACACTGC TAGGATTTTT CCTCGCGAAG ATTCACAAAA TTGACCCAGA TGTTGTTGTG 
GGTCACAATA TTTATGGATT TGATCTGGAA GTGCTGCTTC AAAGAATTAA TGTGTGCAAA 
GTCCCTCACT GGTCCAAGAT AGGTCGACTG AGGAGGTCCA ATATGCCAAA ACTTGGGGGT 
CGAGGAGGAT TTGGTGAAAG GAATGCTGCG TGTGGTCGGA TGATCTGTGA TGTAGAAATT 
TCAGCTAAAG AGTTAATTCG CTGCAAGAGT TACCATTTGT CTGAACTGGT CCATCAGATT 
TTGAAAACAG AGAGGGTAAC AATTCTACCA GAGGAAATTC GAAATTTGTA CAGCGATTCT 
CCTCACTTAC TGTTCATGCT GGAAAACACC TGGACAGATG CCAAGTTCAT TCTTCAGATC 
ATGTGCGAGC TGAATGTTCT GCCACTAGCC CTACAGATTA CAAACATCTC TGGAAATGTT 
ATGTCGAGGA CAATGATGGG TGGACGTTCT GAACGCAATG AGTTCTTACT GCTTCATGCA 
TTTTATGAAA AGGACTACAT AGTCCCAGAC AAACAAGTTT TTAAAAAGCC TCTACAGAAG 
CTGGTGGATG AAGATGAAGA TTTTGAAGAT CAGAATAAAT CAAAGATAGG GAAAAAGAAA 
GCAGCATATG CTGGGGGCTT AGTCTTGGAT CCCAAAGTCG GTTTTTATGA CAAGTTTATT 
TTGCTTCTCG ACTTCAACAG CTTGTACCCA TCAATTATCC AGGAGTTTAA CATCTGCTTT 
ACCACAGTGC AGCGACTGTC ATCAGAAGCA CAGAAAAGGG CAGAGGTGGA AGAAGAGGAA 
GAAATTCCAG AGCTTCCGGA CCCTTCTCTG GAAATGGGAA TTCTACCTAA AGAAATCAGG 
AAACTTGTGG AGAGAAGACG CCAAGTTAAG CAGTTAATGA AACAGCCAGA CTTAAATCCT 
GACCTTTATT TACAGTATGA TATCAGACAG AAAGCTTTGA AACTCACCGC TAACAGTATG 
TATGGCTGCC TAGGATTTTC CTACAGCAGA TTCTATGCCA AGCCTTTGGC TGCTTTGGTG 
ACGCATAAAG GGAGAGAGAT TTTGATGCAT ACCAAGGAGA TGGTGCAAAA GATGAACCTT 
GAAGTGATTT ATGGAGACAC AGATTCCATT ATGATAAACA CTAACAGCAC CAATTTGGAT 
GAAGTCTTCA AGCTGGGGAA CAAGATCAAA AGTGAAGTGA ACAAGTTGTA TAAATTGCTG 
GAGATTGATA TTGATGGAGT CTTCAAGTCC TTGCTACTGT TAAAGAAGAA GAAATATGCT 
GCCCTTACCG TGGAACCAAC AGGAGATGGG AAATATGTAA CTAAACAAGA ACTGAAAGGG 
TTGGATATAG TCCGAAGAGA CTGGTGTGAT CTTGCAAAAG AAACTGGAAA CTATGTAATT 
GGTCAGATTC TTTCTGATCA GCCCCGAGAC ATAATTGTGG AAAATATTCA AAGGCGGCTT 
ATAGAAATTG GAGAAAATGT GATCAATGGC CAGATCCCAG TAAATCAGTT TGAAATAAAT 
AAGGCACTGA CAAAAGATCC ACAGGATTAT CCTGACAAAA AAAGCCTGCC ACATGTGCAT 
GTTGCCATGT GGATAAACTC TCAAGGAGGC CGAAAGGTGA AGGCAGGAGA TACAGTGTCA 
TATATCATTT GTCAGGATGG ATCAAATCTC AGTGCCAGCC AGCGAGCATA TGCTCCAGAA 
CAGTTGCAAA AGCAAGACAA TCTTACTATT GATACTCAAT ACTACTTGTC ACAGCAAATA 
CATCCAGTAG TTGCTAGAAT ATGTGAACCT ATAGAGGGAA TTGATTCTGT TCTTATCGCA 
ACGTGGTTAG GACTGGATCC CTCCCAATTC AGAGTCCATC ACCATTACCA TAAAGATGAG 
GAGAATGATG CCTTGGTTGG TGGCCCAGCT CAGCTCACTG ATGAGGAGAA ATATAGGGAC 
TGTGAAAGAT TCAAGTTTTG CTGCCTCAAG TGTGGAACAG AGAATATTTA TGACAATGTC 
TTTGACTGTT CGGGGCGATT TATTGAGCCC AGCTTGCAAA GGTGCAGTAA AAGTGAATGT 
GAAGAGCCTC CTTTCAACCA TGTGGTTCAA ATGAACAACA AATTACTTTT GGATATTAGA 
CGCTACCTAA AAAAATACTA TAATGGTTGG TTAATATGTG AGGATCCAAC TTGCCAGAAT 
CGAACCCGAC GTCTTCCCCT CAGTTTTTCC CGGAGTGGTC CTATATGCCA GGCCTGTAGG 
AAGGCTATCC TGAGACCAGA GTATTCTGAT AAGGCCCTGT ACACTCAGCT GTGCTTTTAC 
CGGTACATTT TTGATGTGGA GTATGCAACG GATAAAGTGA TTACTGAAGA GGATAGAGAG 
TTCGTAAGGA GGAAGCCCCT CAGGAAGGAA GTGTCCGAGG CCTACAGGAA ACTGAAGAGC 
ACGGTAGACA AATGCCTGTC CCTGAGCAGC TACTCGGAAG TGAACCTCGG CAAACTCTTC 
TCCGTCAGCA TGGGAAGATC CGTGGGAGAG CCCAGCAGTG AAAGACAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015158157.1
CDS	131..4621
Translation	MAEDAMPESGSFATSRSRRERKSKRGQQEALERLKKAKAGEKLKYEVEEFTGVYDEIDEEQYSKIVRERQDDDWIVDDDGIGYVEDGREIFDEDLDDDALGSSKKGKTGKTSTIGKKNVKKSVVAKPNTIKSMFIASAGKKNTDKTVDLSKDDLLGDILQDLNAETVQLSPPPILVLKRKRSAGMPLNPFSVQRQSPKVITPVSKKAPVQLRKEARPPASFHPKHPNYTAKSVTASEENEDTKYVQKATENGRVVKTKEEKAIEDDDQGMMDFDDEDFDEPMEAELMGAVPETAETLKRDKEVEKKEMEQLESKKKEASALSHSLPDISCWDKVEEDEADLVATDVQLDPNLLPLVNGENDDQVLRFYWLDAYEDQYSQPGVVFLFGKVWIESANTYVSCCVTVKNIERTVYLLPRETEMDLSSGKETETPVTIMNVFKEFNDCISPKYKIMKFKSKKVEKYYAFEIPDVPAKSEYLEVKYSAEYPRLPQDLKGETFSHAFGTNTSSLELLLMSRKIKGPCWLEIKNPQPSNQSVSWCKVEAVAMKPGLVNVVKDLPPPPPLVVMSFSMKTIQNPKTHQNEIVAMAALIHQKFPLDKAPPQPPFQTHFCAVSKPNDCIFPYDFKEEIKKKNAKIEIAATERTLLGFFLAKIHKIDPDVVVGHNIYGFDLEVLLQRINVCKVPHWSKIGRLRRSNMPKLGGRGGFGERNAACGRMICDVEISAKELIRCKSYHLSELVHQILKTERVTILPEEIRNLYSDSPHLLFMLENTWTDAKFILQIMCELNVLPLALQITNISGNVMSRTMMGGRSERNEFLLLHAFYEKDYIVPDKQVFKKPLQKLVDEDEDFEDQNKSKIGKKKAAYAGGLVLDPKVGFYDKFILLLDFNSLYPSIIQEFNICFTTVQRLSSEAQKRAEVEEEEEIPELPDPSLEMGILPKEIRKLVERRRQVKQLMKQPDLNPDLYLQYDIRQKALKLTANSMYGCLGFSYSRFYAKPLAALVTHKGREILMHTKEMVQKMNLEVIYGDTDSIMINTNSTNLDEVFKLGNKIKSEVNKLYKLLEIDIDGVFKSLLLLKKKKYAALTVEPTGDGKYVTKQELKGLDIVRRDWCDLAKETGNYVIGQILSDQPRDIIVENIQRRLIEIGENVINGQIPVNQFEINKALTKDPQDYPDKKSLPHVHVAMWINSQGGRKVKAGDTVSYIICQDGSNLSASQRAYAPEQLQKQDNLTIDTQYYLSQQIHPVVARICEPIEGIDSVLIATWLGLDPSQFRVHHHYHKDEENDALVGGPAQLTDEEKYRDCERFKFCCLKCGTENIYDNVFDCSGRFIEPSLQRCSKSECEEPPFNHVVQMNNKLLLDIRRYLKKYYNGWLICEDPTCQNRTRRLPLSFSRSGPICQACRKAILRPEYSDKALYTQLCFYRYIFDVEYATDKVITEEDREFVRRKPLRKEVSEAYRKLKSTVDKCLSLSSYSEVNLGKLFSVSMGRSVGEPSSERQ

Target ORF information:

RefSeq Version	XM_015302671.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus DNA polymerase alpha 1, catalytic subunit (POLA1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015302671.2

ORF Insert Sequence:

ATGGCTGAGG ATGCGATGCC TGAGTCTGGG AGCTTTGCAA CGTCACGTTC TAGGCGTGAG 
AGGAAGTCAA AAAGAGGTCA GCAAGAAGCA CTGGAACGTC TGAAAAAGGC CAAAGCTGGG 
GAAAAATTAA AATACGAGGT TGAGGAGTTC ACTGGTGTTT ACGATGAAAT AGATGAAGAA 
CAGTACTCCA AGATAGTCAG AGAACGTCAG GATGATGACT GGATTGTTGA TGATGATGGG 
ATAGGTTATG TAGAAGATGG ACGGGAAATC TTTGATGAAG ATCTAGACGA TGATGCTCTT 
GGTTCCAGTA AGAAAGGAAA AACCGGCAAA ACTTCTACAA TTGGTAAAAA GAATGTGAAA 
AAATCTGTGG TAGCAAAGCC AAACACAATT AAATCTATGT TTATTGCCAG TGCTGGGAAG 
AAAAACACGG ACAAAACTGT GGACCTGTCA AAGGATGATT TACTAGGGGA TATTCTTCAA 
GATCTAAATG CTGAAACTGT TCAGCTAAGT CCACCACCGA TTTTAGTGCT GAAAAGGAAA 
AGATCTGCTG GAATGCCACT GAATCCTTTC TCTGTACAGC GCCAGTCTCC TAAGGTAATC 
ACCCCTGTAT CAAAGAAAGC TCCTGTTCAA CTCAGGAAAG AAGCTCGTCC TCCAGCTTCA 
TTTCATCCTA AACATCCAAA TTACACAGCA AAATCTGTCA CAGCCTCTGA AGAGAATGAG 
GATACCAAGT ATGTGCAGAA AGCTACAGAA AATGGGAGAG TAGTGAAGAC AAAAGAAGAA 
AAAGCTATTG AAGATGATGA TCAGGGGATG ATGGATTTTG ATGATGAGGA CTTTGATGAA 
CCTATGGAAG CAGAGCTAAT GGGAGCTGTA CCTGAGACAG CTGAAACCTT GAAAAGAGAC 
AAGGAAGTTG AAAAGAAAGA GATGGAGCAG CTGGAATCTA AGAAGAAAGA GGCATCTGCC 
TTAAGTCATT CTCTCCCAGA TATCTCATGT TGGGACAAAG TAGAGGAGGA TGAAGCTGAT 
CTGGTGGCGA CAGATGTTCA GTTGGACCCC AATCTCCTTC CACTTGTGAA TGGGGAAAAT 
GATGATCAAG TCTTAAGATT TTATTGGCTG GATGCTTATG AAGATCAGTA CAGTCAGCCA 
GGTGTGGTAT TTTTGTTTGG CAAAGTTTGG ATTGAATCGG CGAACACCTA TGTCAGCTGT 
TGTGTGACGG TGAAAAACAT TGAGAGAACT GTTTATCTAC TGCCACGTGA AACAGAGATG 
GATCTATCCA GTGGGAAAGA GACAGAGACT CCAGTAACCA TAATGAATGT TTTCAAGGAA 
TTTAATGACT GCATTTCACC AAAGTATAAG ATTATGAAGT TCAAATCCAA GAAAGTGGAA 
AAATACTATG CGTTTGAGAT CCCGGATGTT CCAGCCAAGT CTGAATACTT GGAAGTTAAA 
TATTCAGCTG AATACCCTCG GCTTCCTCAA GACCTGAAAG GAGAAACGTT TTCACACGCG 
TTTGGAACTA ACACTTCTAG CTTGGAGCTT CTCTTGATGA GTAGAAAGAT CAAAGGACCG 
TGCTGGCTGG AAATCAAAAA TCCACAGCCC TCAAATCAGT CAGTCAGCTG GTGTAAGGTA 
GAGGCAGTAG CCATGAAGCC TGGCTTGGTG AATGTGGTTA AAGATCTTCC TCCTCCTCCT 
CCTCTTGTGG TAATGTCCTT CAGCATGAAG ACCATTCAGA ACCCAAAGAC TCACCAGAAT 
GAGATTGTGG CTATGGCAGC TCTGATTCAT CAGAAATTTC CTTTGGATAA AGCTCCACCT 
CAGCCTCCTT TTCAGACACA CTTCTGTGCT GTTTCCAAAC CGAATGATTG CATTTTTCCT 
TATGACTTCA AAGAAGAGAT TAAAAAGAAG AATGCTAAAA TAGAAATTGC TGCAACAGAG 
AGAACACTGC TAGGATTTTT CCTCGCGAAG ATTCACAAAA TTGACCCAGA TGTTGTTGTG 
GGTCACAATA TTTATGGATT TGATCTGGAA GTGCTGCTTC AAAGAATTAA TGTGTGCAAA 
GTCCCTCACT GGTCCAAGAT AGGTCGACTG AGGAGGTCCA ATATGCCAAA ACTTGGGGGT 
CGAGGAGGAT TTGGTGAAAG GAATGCTGCG TGTGGTCGGA TGATCTGTGA TGTAGAAATT 
TCAGCTAAAG AGTTAATTCG CTGCAAGAGT TACCATTTGT CTGAACTGGT CCATCAGATT 
TTGAAAACAG AGAGGGTAAC AATTCTACCA GAGGAAATTC GAAATTTGTA CAGCGATTCT 
CCTCACTTAC TGTTCATGCT GGAAAACACC TGGACAGATG CCAAGTTCAT TCTTCAGATC 
ATGTGCGAGC TGAATGTTCT GCCACTAGCC CTACAGATTA CAAACATCTC TGGAAATGTT 
ATGTCGAGGA CAATGATGGG TGGACGTTCT GAACGCAATG AGTTCTTACT GCTTCATGCA 
TTTTATGAAA AGGACTACAT AGTCCCAGAC AAACAAGTTT TTAAAAAGCC TCTACAGAAG 
CTGGTGGATG AAGATGAAGA TTTTGAAGAT CAGAATAAAT CAAAGATAGG GAAAAAGAAA 
GCAGCATATG CTGGGGGCTT AGTCTTGGAT CCCAAAGTCG GTTTTTATGA CAAGTTTATT 
TTGCTTCTCG ACTTCAACAG CTTGTACCCA TCAATTATCC AGGAGTTTAA CATCTGCTTT 
ACCACAGTGC AGCGACTGTC ATCAGAAGCA CAGAAAAGGG CAGAGGTGGA AGAAGAGGAA 
GAAATTCCAG AGCTTCCGGA CCCTTCTCTG GAAATGGGAA TTCTACCTAA AGAAATCAGG 
AAACTTGTGG AGAGAAGACG CCAAGTTAAG CAGTTAATGA AACAGCCAGA CTTAAATCCT 
GACCTTTATT TACAGTATGA TATCAGACAG AAAGCTTTGA AACTCACCGC TAACAGTATG 
TATGGCTGCC TAGGATTTTC CTACAGCAGA TTCTATGCCA AGCCTTTGGC TGCTTTGGTG 
ACGCATAAAG GGAGAGAGAT TTTGATGCAT ACCAAGGAGA TGGTGCAAAA GATGAACCTT 
GAAGTGATTT ATGGAGACAC AGATTCCATT ATGATAAACA CTAACAGCAC CAATTTGGAT 
GAAGTCTTCA AGCTGGGGAA CAAGATCAAA AGTGAAGTGA ACAAGTTGTA TAAATTGCTG 
GAGATTGATA TTGATGGAGT CTTCAAGTCC TTGCTACTGT TAAAGAAGAA GAAATATGCT 
GCCCTTACCG TGGAACCAAC AGGAGATGGG AAATATGTAA CTAAACAAGA ACTGAAAGGG 
TTGGATATAG TCCGAAGAGA CTGGTGTGAT CTTGCAAAAG AAACTGGAAA CTATGTAATT 
GGTCAGATTC TTTCTGATCA GCCCCGAGAC ATAATTGTGG AAAATATTCA AAGGCGGCTT 
ATAGAAATTG GAGAAAATGT GATCAATGGC CAGATCCCAG TAAATCAGTT TGAAATAAAT 
AAGGCACTGA CAAAAGATCC ACAGGATTAT CCTGACAAAA AAAGCCTGCC ACATGTGCAT 
GTTGCCATGT GGATAAACTC TCAAGGAGGC CGAAAGGTGA AGGCAGGAGA TACAGTGTCA 
TATATCATTT GTCAGGATGG ATCAAATCTC AGTGCCAGCC AGCGAGCATA TGCTCCAGAA 
CAGTTGCAAA AGCAAGACAA TCTTACTATT GATACTCAAT ACTACTTGTC ACAGCAAATA 
CATCCAGTAG TTGCTAGAAT ATGTGAACCT ATAGAGGGAA TTGATTCTGT TCTTATCGCA 
ACGTGGTTAG GACTGGATCC CTCCCAATTC AGAGTCCATC ACCATTACCA TAAAGATGAG 
GAGAATGATG CCTTGGTTGG TGGCCCAGCT CAGCTCACTG ATGAGGAGAA ATATAGGGAC 
TGTGAAAGAT TCAAGTTTTG CTGCCTCAAG TGTGGAACAG AGAATATTTA TGACAATGTC 
TTTGACTGTT CGGGGCGATT TATTGAGCCC AGCTTGCAAA GGTGCAGTAA AAGTGAATGT 
GAAGAGCCTC CTTTCAACCA TGTGGTTCAA ATGAACAACA AATTACTTTT GGATATTAGA 
CGCTACCTAA AAAAATACTA TAATGGTTGG TTAATATGTG AGGATCCAAC TTGCCAGAAT 
CGAACCCGAC GTCTTCCCCT CAGTTTTTCC CGGAGTGGTC CTATATGCCA GGCCTGTAGG 
AAGGCTATCC TGAGACCAGA GTATTCTGAT AAGGCCCTGT ACACTCAGCT GTGCTTTTAC 
CGGTACATTT TTGATGTGGA GTATGCAACG GATAAAGTGA TTACTGAAGA GGATAGAGAG 
TTCGTAAGGA GGAAGCCCCT CAGGAAGGAA GTGTCCGAGG CCTACAGGAA ACTGAAGAGC 
ACGGTAGACA AATGCCTGTC CCTGAGCAGC TACTCGGAAG TGAACCTCGG CAAACTCTTC 
TCCGTCAGCA TGGGAAGATC CGTGGGAGAG CCCAGCAGTG AAAGACAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLA1 ( XM_015302671.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015158157.1 Gallus gallus polymerase (DNA directed), alpha 1, catalytic subunit (POLA1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa30439
Clone ID Related Accession (Same CDS sequence)	XM_015302671.2 , XM_015302671.1
Accession Version	XM_015302671.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	DNA polymerase alpha catalytic subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455744.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAGG ATGCGATGCC TGAGTCTGGG AGCTTTGCAA CGTCACGTTC TAGGCGTGAG 
AGGAAGTCAA AAAGAGGTCA GCAAGAAGCA CTGGAACGTC TGAAAAAGGC CAAAGCTGGG 
GAAAAATTAA AATACGAGGT TGAGGAGTTC ACTGGTGTTT ACGATGAAAT AGATGAAGAA 
CAGTACTCCA AGATAGTCAG AGAACGTCAG GATGATGACT GGATTGTTGA TGATGATGGG 
ATAGGTTATG TAGAAGATGG ACGGGAAATC TTTGATGAAG ATCTAGACGA TGATGCTCTT 
GGTTCCAGTA AGAAAGGAAA AACCGGCAAA ACTTCTACAA TTGGTAAAAA GAATGTGAAA 
AAATCTGTGG TAGCAAAGCC AAACACAATT AAATCTATGT TTATTGCCAG TGCTGGGAAG 
AAAAACACGG ACAAAACTGT GGACCTGTCA AAGGATGATT TACTAGGGGA TATTCTTCAA 
GATCTAAATG CTGAAACTGT TCAGCTAAGT CCACCACCGA TTTTAGTGCT GAAAAGGAAA 
AGATCTGCTG GAATGCCACT GAATCCTTTC TCTGTACAGC GCCAGTCTCC TAAGGTAATC 
ACCCCTGTAT CAAAGAAAGC TCCTGTTCAA CTCAGGAAAG AAGCTCGTCC TCCAGCTTCA 
TTTCATCCTA AACATCCAAA TTACACAGCA AAATCTGTCA CAGCCTCTGA AGAGAATGAG 
GATACCAAGT ATGTGCAGAA AGCTACAGAA AATGGGAGAG TAGTGAAGAC AAAAGAAGAA 
AAAGCTATTG AAGATGATGA TCAGGGGATG ATGGATTTTG ATGATGAGGA CTTTGATGAA 
CCTATGGAAG CAGAGCTAAT GGGAGCTGTA CCTGAGACAG CTGAAACCTT GAAAAGAGAC 
AAGGAAGTTG AAAAGAAAGA GATGGAGCAG CTGGAATCTA AGAAGAAAGA GGCATCTGCC 
TTAAGTCATT CTCTCCCAGA TATCTCATGT TGGGACAAAG TAGAGGAGGA TGAAGCTGAT 
CTGGTGGCGA CAGATGTTCA GTTGGACCCC AATCTCCTTC CACTTGTGAA TGGGGAAAAT 
GATGATCAAG TCTTAAGATT TTATTGGCTG GATGCTTATG AAGATCAGTA CAGTCAGCCA 
GGTGTGGTAT TTTTGTTTGG CAAAGTTTGG ATTGAATCGG CGAACACCTA TGTCAGCTGT 
TGTGTGACGG TGAAAAACAT TGAGAGAACT GTTTATCTAC TGCCACGTGA AACAGAGATG 
GATCTATCCA GTGGGAAAGA GACAGAGACT CCAGTAACCA TAATGAATGT TTTCAAGGAA 
TTTAATGACT GCATTTCACC AAAGTATAAG ATTATGAAGT TCAAATCCAA GAAAGTGGAA 
AAATACTATG CGTTTGAGAT CCCGGATGTT CCAGCCAAGT CTGAATACTT GGAAGTTAAA 
TATTCAGCTG AATACCCTCG GCTTCCTCAA GACCTGAAAG GAGAAACGTT TTCACACGCG 
TTTGGAACTA ACACTTCTAG CTTGGAGCTT CTCTTGATGA GTAGAAAGAT CAAAGGACCG 
TGCTGGCTGG AAATCAAAAA TCCACAGCCC TCAAATCAGT CAGTCAGCTG GTGTAAGGTA 
GAGGCAGTAG CCATGAAGCC TGGCTTGGTG AATGTGGTTA AAGATCTTCC TCCTCCTCCT 
CCTCTTGTGG TAATGTCCTT CAGCATGAAG ACCATTCAGA ACCCAAAGAC TCACCAGAAT 
GAGATTGTGG CTATGGCAGC TCTGATTCAT CAGAAATTTC CTTTGGATAA AGCTCCACCT 
CAGCCTCCTT TTCAGACACA CTTCTGTGCT GTTTCCAAAC CGAATGATTG CATTTTTCCT 
TATGACTTCA AAGAAGAGAT TAAAAAGAAG AATGCTAAAA TAGAAATTGC TGCAACAGAG 
AGAACACTGC TAGGATTTTT CCTCGCGAAG ATTCACAAAA TTGACCCAGA TGTTGTTGTG 
GGTCACAATA TTTATGGATT TGATCTGGAA GTGCTGCTTC AAAGAATTAA TGTGTGCAAA 
GTCCCTCACT GGTCCAAGAT AGGTCGACTG AGGAGGTCCA ATATGCCAAA ACTTGGGGGT 
CGAGGAGGAT TTGGTGAAAG GAATGCTGCG TGTGGTCGGA TGATCTGTGA TGTAGAAATT 
TCAGCTAAAG AGTTAATTCG CTGCAAGAGT TACCATTTGT CTGAACTGGT CCATCAGATT 
TTGAAAACAG AGAGGGTAAC AATTCTACCA GAGGAAATTC GAAATTTGTA CAGCGATTCT 
CCTCACTTAC TGTTCATGCT GGAAAACACC TGGACAGATG CCAAGTTCAT TCTTCAGATC 
ATGTGCGAGC TGAATGTTCT GCCACTAGCC CTACAGATTA CAAACATCTC TGGAAATGTT 
ATGTCGAGGA CAATGATGGG TGGACGTTCT GAACGCAATG AGTTCTTACT GCTTCATGCA 
TTTTATGAAA AGGACTACAT AGTCCCAGAC AAACAAGTTT TTAAAAAGCC TCTACAGAAG 
CTGGTGGATG AAGATGAAGA TTTTGAAGAT CAGAATAAAT CAAAGATAGG GAAAAAGAAA 
GCAGCATATG CTGGGGGCTT AGTCTTGGAT CCCAAAGTCG GTTTTTATGA CAAGTTTATT 
TTGCTTCTCG ACTTCAACAG CTTGTACCCA TCAATTATCC AGGAGTTTAA CATCTGCTTT 
ACCACAGTGC AGCGACTGTC ATCAGAAGCA CAGAAAAGGG CAGAGGTGGA AGAAGAGGAA 
GAAATTCCAG AGCTTCCGGA CCCTTCTCTG GAAATGGGAA TTCTACCTAA AGAAATCAGG 
AAACTTGTGG AGAGAAGACG CCAAGTTAAG CAGTTAATGA AACAGCCAGA CTTAAATCCT 
GACCTTTATT TACAGTATGA TATCAGACAG AAAGCTTTGA AACTCACCGC TAACAGTATG 
TATGGCTGCC TAGGATTTTC CTACAGCAGA TTCTATGCCA AGCCTTTGGC TGCTTTGGTG 
ACGCATAAAG GGAGAGAGAT TTTGATGCAT ACCAAGGAGA TGGTGCAAAA GATGAACCTT 
GAAGTGATTT ATGGAGACAC AGATTCCATT ATGATAAACA CTAACAGCAC CAATTTGGAT 
GAAGTCTTCA AGCTGGGGAA CAAGATCAAA AGTGAAGTGA ACAAGTTGTA TAAATTGCTG 
GAGATTGATA TTGATGGAGT CTTCAAGTCC TTGCTACTGT TAAAGAAGAA GAAATATGCT 
GCCCTTACCG TGGAACCAAC AGGAGATGGG AAATATGTAA CTAAACAAGA ACTGAAAGGG 
TTGGATATAG TCCGAAGAGA CTGGTGTGAT CTTGCAAAAG AAACTGGAAA CTATGTAATT 
GGTCAGATTC TTTCTGATCA GCCCCGAGAC ATAATTGTGG AAAATATTCA AAGGCGGCTT 
ATAGAAATTG GAGAAAATGT GATCAATGGC CAGATCCCAG TAAATCAGTT TGAAATAAAT 
AAGGCACTGA CAAAAGATCC ACAGGATTAT CCTGACAAAA AAAGCCTGCC ACATGTGCAT 
GTTGCCATGT GGATAAACTC TCAAGGAGGC CGAAAGGTGA AGGCAGGAGA TACAGTGTCA 
TATATCATTT GTCAGGATGG ATCAAATCTC AGTGCCAGCC AGCGAGCATA TGCTCCAGAA 
CAGTTGCAAA AGCAAGACAA TCTTACTATT GATACTCAAT ACTACTTGTC ACAGCAAATA 
CATCCAGTAG TTGCTAGAAT ATGTGAACCT ATAGAGGGAA TTGATTCTGT TCTTATCGCA 
ACGTGGTTAG GACTGGATCC CTCCCAATTC AGAGTCCATC ACCATTACCA TAAAGATGAG 
GAGAATGATG CCTTGGTTGG TGGCCCAGCT CAGCTCACTG ATGAGGAGAA ATATAGGGAC 
TGTGAAAGAT TCAAGTTTTG CTGCCTCAAG TGTGGAACAG AGAATATTTA TGACAATGTC 
TTTGACTGTT CGGGGCGATT TATTGAGCCC AGCTTGCAAA GGTGCAGTAA AAGTGAATGT 
GAAGAGCCTC CTTTCAACCA TGTGGTTCAA ATGAACAACA AATTACTTTT GGATATTAGA 
CGCTACCTAA AAAAATACTA TAATGGTTGG TTAATATGTG AGGATCCAAC TTGCCAGAAT 
CGAACCCGAC GTCTTCCCCT CAGTTTTTCC CGGAGTGGTC CTATATGCCA GGCCTGTAGG 
AAGGCTATCC TGAGACCAGA GTATTCTGAT AAGGCCCTGT ACACTCAGCT GTGCTTTTAC 
CGGTACATTT TTGATGTGGA GTATGCAACG GATAAAGTGA TTACTGAAGA GGATAGAGAG 
TTCGTAAGGA GGAAGCCCCT CAGGAAGGAA GTGTCCGAGG CCTACAGGAA ACTGAAGAGC 
ACGGTAGACA AATGCCTGTC CCTGAGCAGC TACTCGGAAG TGAACCTCGG CAAACTCTTC 
TCCGTCAGCA TGGGAAGATC CGTGGGAGAG CCCAGCAGTG AAAGACAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015158157.1
CDS	131..4621
Translation	MAEDAMPESGSFATSRSRRERKSKRGQQEALERLKKAKAGEKLKYEVEEFTGVYDEIDEEQYSKIVRERQDDDWIVDDDGIGYVEDGREIFDEDLDDDALGSSKKGKTGKTSTIGKKNVKKSVVAKPNTIKSMFIASAGKKNTDKTVDLSKDDLLGDILQDLNAETVQLSPPPILVLKRKRSAGMPLNPFSVQRQSPKVITPVSKKAPVQLRKEARPPASFHPKHPNYTAKSVTASEENEDTKYVQKATENGRVVKTKEEKAIEDDDQGMMDFDDEDFDEPMEAELMGAVPETAETLKRDKEVEKKEMEQLESKKKEASALSHSLPDISCWDKVEEDEADLVATDVQLDPNLLPLVNGENDDQVLRFYWLDAYEDQYSQPGVVFLFGKVWIESANTYVSCCVTVKNIERTVYLLPRETEMDLSSGKETETPVTIMNVFKEFNDCISPKYKIMKFKSKKVEKYYAFEIPDVPAKSEYLEVKYSAEYPRLPQDLKGETFSHAFGTNTSSLELLLMSRKIKGPCWLEIKNPQPSNQSVSWCKVEAVAMKPGLVNVVKDLPPPPPLVVMSFSMKTIQNPKTHQNEIVAMAALIHQKFPLDKAPPQPPFQTHFCAVSKPNDCIFPYDFKEEIKKKNAKIEIAATERTLLGFFLAKIHKIDPDVVVGHNIYGFDLEVLLQRINVCKVPHWSKIGRLRRSNMPKLGGRGGFGERNAACGRMICDVEISAKELIRCKSYHLSELVHQILKTERVTILPEEIRNLYSDSPHLLFMLENTWTDAKFILQIMCELNVLPLALQITNISGNVMSRTMMGGRSERNEFLLLHAFYEKDYIVPDKQVFKKPLQKLVDEDEDFEDQNKSKIGKKKAAYAGGLVLDPKVGFYDKFILLLDFNSLYPSIIQEFNICFTTVQRLSSEAQKRAEVEEEEEIPELPDPSLEMGILPKEIRKLVERRRQVKQLMKQPDLNPDLYLQYDIRQKALKLTANSMYGCLGFSYSRFYAKPLAALVTHKGREILMHTKEMVQKMNLEVIYGDTDSIMINTNSTNLDEVFKLGNKIKSEVNKLYKLLEIDIDGVFKSLLLLKKKKYAALTVEPTGDGKYVTKQELKGLDIVRRDWCDLAKETGNYVIGQILSDQPRDIIVENIQRRLIEIGENVINGQIPVNQFEINKALTKDPQDYPDKKSLPHVHVAMWINSQGGRKVKAGDTVSYIICQDGSNLSASQRAYAPEQLQKQDNLTIDTQYYLSQQIHPVVARICEPIEGIDSVLIATWLGLDPSQFRVHHHYHKDEENDALVGGPAQLTDEEKYRDCERFKFCCLKCGTENIYDNVFDCSGRFIEPSLQRCSKSECEEPPFNHVVQMNNKLLLDIRRYLKKYYNGWLICEDPTCQNRTRRLPLSFSRSGPICQACRKAILRPEYSDKALYTQLCFYRYIFDVEYATDKVITEEDREFVRRKPLRKEVSEAYRKLKSTVDKCLSLSSYSEVNLGKLFSVSMGRSVGEPSSERQ

Target ORF information:

RefSeq Version	XM_015302671.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus polymerase (DNA directed), alpha 1, catalytic subunit (POLA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015302671.1

ORF Insert Sequence:

ATGGCTGAGG ATGCGATGCC TGAGTCTGGG AGCTTTGCAA CGTCACGTTC TAGGCGTGAG 
AGGAAGTCAA AAAGAGGTCA GCAAGAAGCA CTGGAACGTC TGAAAAAGGC CAAAGCTGGG 
GAAAAATTAA AATACGAGGT TGAGGAGTTC ACTGGTGTTT ACGATGAAAT AGATGAAGAA 
CAGTACTCCA AGATAGTCAG AGAACGTCAG GATGATGACT GGATTGTTGA TGATGATGGG 
ATAGGTTATG TAGAAGATGG ACGGGAAATC TTTGATGAAG ATCTAGACGA TGATGCTCTT 
GGTTCCAGTA AGAAAGGAAA AACCGGCAAA ACTTCTACAA TTGGTAAAAA GAATGTGAAA 
AAATCTGTGG TAGCAAAGCC AAACACAATT AAATCTATGT TTATTGCCAG TGCTGGGAAG 
AAAAACACGG ACAAAACTGT GGACCTGTCA AAGGATGATT TACTAGGGGA TATTCTTCAA 
GATCTAAATG CTGAAACTGT TCAGCTAAGT CCACCACCGA TTTTAGTGCT GAAAAGGAAA 
AGATCTGCTG GAATGCCACT GAATCCTTTC TCTGTACAGC GCCAGTCTCC TAAGGTAATC 
ACCCCTGTAT CAAAGAAAGC TCCTGTTCAA CTCAGGAAAG AAGCTCGTCC TCCAGCTTCA 
TTTCATCCTA AACATCCAAA TTACACAGCA AAATCTGTCA CAGCCTCTGA AGAGAATGAG 
GATACCAAGT ATGTGCAGAA AGCTACAGAA AATGGGAGAG TAGTGAAGAC AAAAGAAGAA 
AAAGCTATTG AAGATGATGA TCAGGGGATG ATGGATTTTG ATGATGAGGA CTTTGATGAA 
CCTATGGAAG CAGAGCTAAT GGGAGCTGTA CCTGAGACAG CTGAAACCTT GAAAAGAGAC 
AAGGAAGTTG AAAAGAAAGA GATGGAGCAG CTGGAATCTA AGAAGAAAGA GGCATCTGCC 
TTAAGTCATT CTCTCCCAGA TATCTCATGT TGGGACAAAG TAGAGGAGGA TGAAGCTGAT 
CTGGTGGCGA CAGATGTTCA GTTGGACCCC AATCTCCTTC CACTTGTGAA TGGGGAAAAT 
GATGATCAAG TCTTAAGATT TTATTGGCTG GATGCTTATG AAGATCAGTA CAGTCAGCCA 
GGTGTGGTAT TTTTGTTTGG CAAAGTTTGG ATTGAATCGG CGAACACCTA TGTCAGCTGT 
TGTGTGACGG TGAAAAACAT TGAGAGAACT GTTTATCTAC TGCCACGTGA AACAGAGATG 
GATCTATCCA GTGGGAAAGA GACAGAGACT CCAGTAACCA TAATGAATGT TTTCAAGGAA 
TTTAATGACT GCATTTCACC AAAGTATAAG ATTATGAAGT TCAAATCCAA GAAAGTGGAA 
AAATACTATG CGTTTGAGAT CCCGGATGTT CCAGCCAAGT CTGAATACTT GGAAGTTAAA 
TATTCAGCTG AATACCCTCG GCTTCCTCAA GACCTGAAAG GAGAAACGTT TTCACACGCG 
TTTGGAACTA ACACTTCTAG CTTGGAGCTT CTCTTGATGA GTAGAAAGAT CAAAGGACCG 
TGCTGGCTGG AAATCAAAAA TCCACAGCCC TCAAATCAGT CAGTCAGCTG GTGTAAGGTA 
GAGGCAGTAG CCATGAAGCC TGGCTTGGTG AATGTGGTTA AAGATCTTCC TCCTCCTCCT 
CCTCTTGTGG TAATGTCCTT CAGCATGAAG ACCATTCAGA ACCCAAAGAC TCACCAGAAT 
GAGATTGTGG CTATGGCAGC TCTGATTCAT CAGAAATTTC CTTTGGATAA AGCTCCACCT 
CAGCCTCCTT TTCAGACACA CTTCTGTGCT GTTTCCAAAC CGAATGATTG CATTTTTCCT 
TATGACTTCA AAGAAGAGAT TAAAAAGAAG AATGCTAAAA TAGAAATTGC TGCAACAGAG 
AGAACACTGC TAGGATTTTT CCTCGCGAAG ATTCACAAAA TTGACCCAGA TGTTGTTGTG 
GGTCACAATA TTTATGGATT TGATCTGGAA GTGCTGCTTC AAAGAATTAA TGTGTGCAAA 
GTCCCTCACT GGTCCAAGAT AGGTCGACTG AGGAGGTCCA ATATGCCAAA ACTTGGGGGT 
CGAGGAGGAT TTGGTGAAAG GAATGCTGCG TGTGGTCGGA TGATCTGTGA TGTAGAAATT 
TCAGCTAAAG AGTTAATTCG CTGCAAGAGT TACCATTTGT CTGAACTGGT CCATCAGATT 
TTGAAAACAG AGAGGGTAAC AATTCTACCA GAGGAAATTC GAAATTTGTA CAGCGATTCT 
CCTCACTTAC TGTTCATGCT GGAAAACACC TGGACAGATG CCAAGTTCAT TCTTCAGATC 
ATGTGCGAGC TGAATGTTCT GCCACTAGCC CTACAGATTA CAAACATCTC TGGAAATGTT 
ATGTCGAGGA CAATGATGGG TGGACGTTCT GAACGCAATG AGTTCTTACT GCTTCATGCA 
TTTTATGAAA AGGACTACAT AGTCCCAGAC AAACAAGTTT TTAAAAAGCC TCTACAGAAG 
CTGGTGGATG AAGATGAAGA TTTTGAAGAT CAGAATAAAT CAAAGATAGG GAAAAAGAAA 
GCAGCATATG CTGGGGGCTT AGTCTTGGAT CCCAAAGTCG GTTTTTATGA CAAGTTTATT 
TTGCTTCTCG ACTTCAACAG CTTGTACCCA TCAATTATCC AGGAGTTTAA CATCTGCTTT 
ACCACAGTGC AGCGACTGTC ATCAGAAGCA CAGAAAAGGG CAGAGGTGGA AGAAGAGGAA 
GAAATTCCAG AGCTTCCGGA CCCTTCTCTG GAAATGGGAA TTCTACCTAA AGAAATCAGG 
AAACTTGTGG AGAGAAGACG CCAAGTTAAG CAGTTAATGA AACAGCCAGA CTTAAATCCT 
GACCTTTATT TACAGTATGA TATCAGACAG AAAGCTTTGA AACTCACCGC TAACAGTATG 
TATGGCTGCC TAGGATTTTC CTACAGCAGA TTCTATGCCA AGCCTTTGGC TGCTTTGGTG 
ACGCATAAAG GGAGAGAGAT TTTGATGCAT ACCAAGGAGA TGGTGCAAAA GATGAACCTT 
GAAGTGATTT ATGGAGACAC AGATTCCATT ATGATAAACA CTAACAGCAC CAATTTGGAT 
GAAGTCTTCA AGCTGGGGAA CAAGATCAAA AGTGAAGTGA ACAAGTTGTA TAAATTGCTG 
GAGATTGATA TTGATGGAGT CTTCAAGTCC TTGCTACTGT TAAAGAAGAA GAAATATGCT 
GCCCTTACCG TGGAACCAAC AGGAGATGGG AAATATGTAA CTAAACAAGA ACTGAAAGGG 
TTGGATATAG TCCGAAGAGA CTGGTGTGAT CTTGCAAAAG AAACTGGAAA CTATGTAATT 
GGTCAGATTC TTTCTGATCA GCCCCGAGAC ATAATTGTGG AAAATATTCA AAGGCGGCTT 
ATAGAAATTG GAGAAAATGT GATCAATGGC CAGATCCCAG TAAATCAGTT TGAAATAAAT 
AAGGCACTGA CAAAAGATCC ACAGGATTAT CCTGACAAAA AAAGCCTGCC ACATGTGCAT 
GTTGCCATGT GGATAAACTC TCAAGGAGGC CGAAAGGTGA AGGCAGGAGA TACAGTGTCA 
TATATCATTT GTCAGGATGG ATCAAATCTC AGTGCCAGCC AGCGAGCATA TGCTCCAGAA 
CAGTTGCAAA AGCAAGACAA TCTTACTATT GATACTCAAT ACTACTTGTC ACAGCAAATA 
CATCCAGTAG TTGCTAGAAT ATGTGAACCT ATAGAGGGAA TTGATTCTGT TCTTATCGCA 
ACGTGGTTAG GACTGGATCC CTCCCAATTC AGAGTCCATC ACCATTACCA TAAAGATGAG 
GAGAATGATG CCTTGGTTGG TGGCCCAGCT CAGCTCACTG ATGAGGAGAA ATATAGGGAC 
TGTGAAAGAT TCAAGTTTTG CTGCCTCAAG TGTGGAACAG AGAATATTTA TGACAATGTC 
TTTGACTGTT CGGGGCGATT TATTGAGCCC AGCTTGCAAA GGTGCAGTAA AAGTGAATGT 
GAAGAGCCTC CTTTCAACCA TGTGGTTCAA ATGAACAACA AATTACTTTT GGATATTAGA 
CGCTACCTAA AAAAATACTA TAATGGTTGG TTAATATGTG AGGATCCAAC TTGCCAGAAT 
CGAACCCGAC GTCTTCCCCT CAGTTTTTCC CGGAGTGGTC CTATATGCCA GGCCTGTAGG 
AAGGCTATCC TGAGACCAGA GTATTCTGAT AAGGCCCTGT ACACTCAGCT GTGCTTTTAC 
CGGTACATTT TTGATGTGGA GTATGCAACG GATAAAGTGA TTACTGAAGA GGATAGAGAG 
TTCGTAAGGA GGAAGCCCCT CAGGAAGGAA GTGTCCGAGG CCTACAGGAA ACTGAAGAGC 
ACGGTAGACA AATGCCTGTC CCTGAGCAGC TACTCGGAAG TGAACCTCGG CAAACTCTTC 
TCCGTCAGCA TGGGAAGATC CGTGGGAGAG CCCAGCAGTG AAAGACAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLA1 ( XM_015302675.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015158161.1 Gallus gallus polymerase (DNA directed), alpha 1, catalytic subunit (POLA1), transcript variant X2, mRNA.

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CloneID	OGa30440
Clone ID Related Accession (Same CDS sequence)	XM_015302675.1 , XM_015302675.2
Accession Version	XM_015302675.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4476bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	DNA polymerase alpha catalytic subunit isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455744.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGAGT CTGGGAGCTT TGCAACGTCA CGTTCTAGGC GTGAGAGGAA GTCAAAAAGA 
GGTCAGCAAG AAGCACTGGA ACGTCTGAAA AAGGCCAAAG CTGGGGAAAA ATTAAAATAC 
GAGGTTGAGG AGTTCACTGG TGTTTACGAT GAAATAGATG AAGAACAGTA CTCCAAGATA 
GTCAGAGAAC GTCAGGATGA TGACTGGATT GTTGATGATG ATGGGATAGG TTATGTAGAA 
GATGGACGGG AAATCTTTGA TGAAGATCTA GACGATGATG CTCTTGGTTC CAGTAAGAAA 
GGAAAAACCG GCAAAACTTC TACAATTGGT AAAAAGAATG TGAAAAAATC TGTGGTAGCA 
AAGCCAAACA CAATTAAATC TATGTTTATT GCCAGTGCTG GGAAGAAAAA CACGGACAAA 
ACTGTGGACC TGTCAAAGGA TGATTTACTA GGGGATATTC TTCAAGATCT AAATGCTGAA 
ACTGTTCAGC TAAGTCCACC ACCGATTTTA GTGCTGAAAA GGAAAAGATC TGCTGGAATG 
CCACTGAATC CTTTCTCTGT ACAGCGCCAG TCTCCTAAGG TAATCACCCC TGTATCAAAG 
AAAGCTCCTG TTCAACTCAG GAAAGAAGCT CGTCCTCCAG CTTCATTTCA TCCTAAACAT 
CCAAATTACA CAGCAAAATC TGTCACAGCC TCTGAAGAGA ATGAGGATAC CAAGTATGTG 
CAGAAAGCTA CAGAAAATGG GAGAGTAGTG AAGACAAAAG AAGAAAAAGC TATTGAAGAT 
GATGATCAGG GGATGATGGA TTTTGATGAT GAGGACTTTG ATGAACCTAT GGAAGCAGAG 
CTAATGGGAG CTGTACCTGA GACAGCTGAA ACCTTGAAAA GAGACAAGGA AGTTGAAAAG 
AAAGAGATGG AGCAGCTGGA ATCTAAGAAG AAAGAGGCAT CTGCCTTAAG TCATTCTCTC 
CCAGATATCT CATGTTGGGA CAAAGTAGAG GAGGATGAAG CTGATCTGGT GGCGACAGAT 
GTTCAGTTGG ACCCCAATCT CCTTCCACTT GTGAATGGGG AAAATGATGA TCAAGTCTTA 
AGATTTTATT GGCTGGATGC TTATGAAGAT CAGTACAGTC AGCCAGGTGT GGTATTTTTG 
TTTGGCAAAG TTTGGATTGA ATCGGCGAAC ACCTATGTCA GCTGTTGTGT GACGGTGAAA 
AACATTGAGA GAACTGTTTA TCTACTGCCA CGTGAAACAG AGATGGATCT ATCCAGTGGG 
AAAGAGACAG AGACTCCAGT AACCATAATG AATGTTTTCA AGGAATTTAA TGACTGCATT 
TCACCAAAGT ATAAGATTAT GAAGTTCAAA TCCAAGAAAG TGGAAAAATA CTATGCGTTT 
GAGATCCCGG ATGTTCCAGC CAAGTCTGAA TACTTGGAAG TTAAATATTC AGCTGAATAC 
CCTCGGCTTC CTCAAGACCT GAAAGGAGAA ACGTTTTCAC ACGCGTTTGG AACTAACACT 
TCTAGCTTGG AGCTTCTCTT GATGAGTAGA AAGATCAAAG GACCGTGCTG GCTGGAAATC 
AAAAATCCAC AGCCCTCAAA TCAGTCAGTC AGCTGGTGTA AGGTAGAGGC AGTAGCCATG 
AAGCCTGGCT TGGTGAATGT GGTTAAAGAT CTTCCTCCTC CTCCTCCTCT TGTGGTAATG 
TCCTTCAGCA TGAAGACCAT TCAGAACCCA AAGACTCACC AGAATGAGAT TGTGGCTATG 
GCAGCTCTGA TTCATCAGAA ATTTCCTTTG GATAAAGCTC CACCTCAGCC TCCTTTTCAG 
ACACACTTCT GTGCTGTTTC CAAACCGAAT GATTGCATTT TTCCTTATGA CTTCAAAGAA 
GAGATTAAAA AGAAGAATGC TAAAATAGAA ATTGCTGCAA CAGAGAGAAC ACTGCTAGGA 
TTTTTCCTCG CGAAGATTCA CAAAATTGAC CCAGATGTTG TTGTGGGTCA CAATATTTAT 
GGATTTGATC TGGAAGTGCT GCTTCAAAGA ATTAATGTGT GCAAAGTCCC TCACTGGTCC 
AAGATAGGTC GACTGAGGAG GTCCAATATG CCAAAACTTG GGGGTCGAGG AGGATTTGGT 
GAAAGGAATG CTGCGTGTGG TCGGATGATC TGTGATGTAG AAATTTCAGC TAAAGAGTTA 
ATTCGCTGCA AGAGTTACCA TTTGTCTGAA CTGGTCCATC AGATTTTGAA AACAGAGAGG 
GTAACAATTC TACCAGAGGA AATTCGAAAT TTGTACAGCG ATTCTCCTCA CTTACTGTTC 
ATGCTGGAAA ACACCTGGAC AGATGCCAAG TTCATTCTTC AGATCATGTG CGAGCTGAAT 
GTTCTGCCAC TAGCCCTACA GATTACAAAC ATCTCTGGAA ATGTTATGTC GAGGACAATG 
ATGGGTGGAC GTTCTGAACG CAATGAGTTC TTACTGCTTC ATGCATTTTA TGAAAAGGAC 
TACATAGTCC CAGACAAACA AGTTTTTAAA AAGCCTCTAC AGAAGCTGGT GGATGAAGAT 
GAAGATTTTG AAGATCAGAA TAAATCAAAG ATAGGGAAAA AGAAAGCAGC ATATGCTGGG 
GGCTTAGTCT TGGATCCCAA AGTCGGTTTT TATGACAAGT TTATTTTGCT TCTCGACTTC 
AACAGCTTGT ACCCATCAAT TATCCAGGAG TTTAACATCT GCTTTACCAC AGTGCAGCGA 
CTGTCATCAG AAGCACAGAA AAGGGCAGAG GTGGAAGAAG AGGAAGAAAT TCCAGAGCTT 
CCGGACCCTT CTCTGGAAAT GGGAATTCTA CCTAAAGAAA TCAGGAAACT TGTGGAGAGA 
AGACGCCAAG TTAAGCAGTT AATGAAACAG CCAGACTTAA ATCCTGACCT TTATTTACAG 
TATGATATCA GACAGAAAGC TTTGAAACTC ACCGCTAACA GTATGTATGG CTGCCTAGGA 
TTTTCCTACA GCAGATTCTA TGCCAAGCCT TTGGCTGCTT TGGTGACGCA TAAAGGGAGA 
GAGATTTTGA TGCATACCAA GGAGATGGTG CAAAAGATGA ACCTTGAAGT GATTTATGGA 
GACACAGATT CCATTATGAT AAACACTAAC AGCACCAATT TGGATGAAGT CTTCAAGCTG 
GGGAACAAGA TCAAAAGTGA AGTGAACAAG TTGTATAAAT TGCTGGAGAT TGATATTGAT 
GGAGTCTTCA AGTCCTTGCT ACTGTTAAAG AAGAAGAAAT ATGCTGCCCT TACCGTGGAA 
CCAACAGGAG ATGGGAAATA TGTAACTAAA CAAGAACTGA AAGGGTTGGA TATAGTCCGA 
AGAGACTGGT GTGATCTTGC AAAAGAAACT GGAAACTATG TAATTGGTCA GATTCTTTCT 
GATCAGCCCC GAGACATAAT TGTGGAAAAT ATTCAAAGGC GGCTTATAGA AATTGGAGAA 
AATGTGATCA ATGGCCAGAT CCCAGTAAAT CAGTTTGAAA TAAATAAGGC ACTGACAAAA 
GATCCACAGG ATTATCCTGA CAAAAAAAGC CTGCCACATG TGCATGTTGC CATGTGGATA 
AACTCTCAAG GAGGCCGAAA GGTGAAGGCA GGAGATACAG TGTCATATAT CATTTGTCAG 
GATGGATCAA ATCTCAGTGC CAGCCAGCGA GCATATGCTC CAGAACAGTT GCAAAAGCAA 
GACAATCTTA CTATTGATAC TCAATACTAC TTGTCACAGC AAATACATCC AGTAGTTGCT 
AGAATATGTG AACCTATAGA GGGAATTGAT TCTGTTCTTA TCGCAACGTG GTTAGGACTG 
GATCCCTCCC AATTCAGAGT CCATCACCAT TACCATAAAG ATGAGGAGAA TGATGCCTTG 
GTTGGTGGCC CAGCTCAGCT CACTGATGAG GAGAAATATA GGGACTGTGA AAGATTCAAG 
TTTTGCTGCC TCAAGTGTGG AACAGAGAAT ATTTATGACA ATGTCTTTGA CTGTTCGGGG 
CGATTTATTG AGCCCAGCTT GCAAAGGTGC AGTAAAAGTG AATGTGAAGA GCCTCCTTTC 
AACCATGTGG TTCAAATGAA CAACAAATTA CTTTTGGATA TTAGACGCTA CCTAAAAAAA 
TACTATAATG GTTGGTTAAT ATGTGAGGAT CCAACTTGCC AGAATCGAAC CCGACGTCTT 
CCCCTCAGTT TTTCCCGGAG TGGTCCTATA TGCCAGGCCT GTAGGAAGGC TATCCTGAGA 
CCAGAGTATT CTGATAAGGC CCTGTACACT CAGCTGTGCT TTTACCGGTA CATTTTTGAT 
GTGGAGTATG CAACGGATAA AGTGATTACT GAAGAGGATA GAGAGTTCGT AAGGAGGAAG 
CCCCTCAGGA AGGAAGTGTC CGAGGCCTAC AGGAAACTGA AGAGCACGGT AGACAAATGC 
CTGTCCCTGA GCAGCTACTC GGAAGTGAAC CTCGGCAAAC TCTTCTCCGT CAGCATGGGA 
AGATCCGTGG GAGAGCCCAG CAGTGAAAGA CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015158161.1
CDS	92..4567
Translation	MPESGSFATSRSRRERKSKRGQQEALERLKKAKAGEKLKYEVEEFTGVYDEIDEEQYSKIVRERQDDDWIVDDDGIGYVEDGREIFDEDLDDDALGSSKKGKTGKTSTIGKKNVKKSVVAKPNTIKSMFIASAGKKNTDKTVDLSKDDLLGDILQDLNAETVQLSPPPILVLKRKRSAGMPLNPFSVQRQSPKVITPVSKKAPVQLRKEARPPASFHPKHPNYTAKSVTASEENEDTKYVQKATENGRVVKTKEEKAIEDDDQGMMDFDDEDFDEPMEAELMGAVPETAETLKRDKEVEKKEMEQLESKKKEASALSHSLPDISCWDKVEEDEADLVATDVQLDPNLLPLVNGENDDQVLRFYWLDAYEDQYSQPGVVFLFGKVWIESANTYVSCCVTVKNIERTVYLLPRETEMDLSSGKETETPVTIMNVFKEFNDCISPKYKIMKFKSKKVEKYYAFEIPDVPAKSEYLEVKYSAEYPRLPQDLKGETFSHAFGTNTSSLELLLMSRKIKGPCWLEIKNPQPSNQSVSWCKVEAVAMKPGLVNVVKDLPPPPPLVVMSFSMKTIQNPKTHQNEIVAMAALIHQKFPLDKAPPQPPFQTHFCAVSKPNDCIFPYDFKEEIKKKNAKIEIAATERTLLGFFLAKIHKIDPDVVVGHNIYGFDLEVLLQRINVCKVPHWSKIGRLRRSNMPKLGGRGGFGERNAACGRMICDVEISAKELIRCKSYHLSELVHQILKTERVTILPEEIRNLYSDSPHLLFMLENTWTDAKFILQIMCELNVLPLALQITNISGNVMSRTMMGGRSERNEFLLLHAFYEKDYIVPDKQVFKKPLQKLVDEDEDFEDQNKSKIGKKKAAYAGGLVLDPKVGFYDKFILLLDFNSLYPSIIQEFNICFTTVQRLSSEAQKRAEVEEEEEIPELPDPSLEMGILPKEIRKLVERRRQVKQLMKQPDLNPDLYLQYDIRQKALKLTANSMYGCLGFSYSRFYAKPLAALVTHKGREILMHTKEMVQKMNLEVIYGDTDSIMINTNSTNLDEVFKLGNKIKSEVNKLYKLLEIDIDGVFKSLLLLKKKKYAALTVEPTGDGKYVTKQELKGLDIVRRDWCDLAKETGNYVIGQILSDQPRDIIVENIQRRLIEIGENVINGQIPVNQFEINKALTKDPQDYPDKKSLPHVHVAMWINSQGGRKVKAGDTVSYIICQDGSNLSASQRAYAPEQLQKQDNLTIDTQYYLSQQIHPVVARICEPIEGIDSVLIATWLGLDPSQFRVHHHYHKDEENDALVGGPAQLTDEEKYRDCERFKFCCLKCGTENIYDNVFDCSGRFIEPSLQRCSKSECEEPPFNHVVQMNNKLLLDIRRYLKKYYNGWLICEDPTCQNRTRRLPLSFSRSGPICQACRKAILRPEYSDKALYTQLCFYRYIFDVEYATDKVITEEDREFVRRKPLRKEVSEAYRKLKSTVDKCLSLSSYSEVNLGKLFSVSMGRSVGEPSSERQ

Target ORF information:

RefSeq Version	XM_015302675.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus polymerase (DNA directed), alpha 1, catalytic subunit (POLA1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015302675.1

ORF Insert Sequence:

ATGCCTGAGT CTGGGAGCTT TGCAACGTCA CGTTCTAGGC GTGAGAGGAA GTCAAAAAGA 
GGTCAGCAAG AAGCACTGGA ACGTCTGAAA AAGGCCAAAG CTGGGGAAAA ATTAAAATAC 
GAGGTTGAGG AGTTCACTGG TGTTTACGAT GAAATAGATG AAGAACAGTA CTCCAAGATA 
GTCAGAGAAC GTCAGGATGA TGACTGGATT GTTGATGATG ATGGGATAGG TTATGTAGAA 
GATGGACGGG AAATCTTTGA TGAAGATCTA GACGATGATG CTCTTGGTTC CAGTAAGAAA 
GGAAAAACCG GCAAAACTTC TACAATTGGT AAAAAGAATG TGAAAAAATC TGTGGTAGCA 
AAGCCAAACA CAATTAAATC TATGTTTATT GCCAGTGCTG GGAAGAAAAA CACGGACAAA 
ACTGTGGACC TGTCAAAGGA TGATTTACTA GGGGATATTC TTCAAGATCT AAATGCTGAA 
ACTGTTCAGC TAAGTCCACC ACCGATTTTA GTGCTGAAAA GGAAAAGATC TGCTGGAATG 
CCACTGAATC CTTTCTCTGT ACAGCGCCAG TCTCCTAAGG TAATCACCCC TGTATCAAAG 
AAAGCTCCTG TTCAACTCAG GAAAGAAGCT CGTCCTCCAG CTTCATTTCA TCCTAAACAT 
CCAAATTACA CAGCAAAATC TGTCACAGCC TCTGAAGAGA ATGAGGATAC CAAGTATGTG 
CAGAAAGCTA CAGAAAATGG GAGAGTAGTG AAGACAAAAG AAGAAAAAGC TATTGAAGAT 
GATGATCAGG GGATGATGGA TTTTGATGAT GAGGACTTTG ATGAACCTAT GGAAGCAGAG 
CTAATGGGAG CTGTACCTGA GACAGCTGAA ACCTTGAAAA GAGACAAGGA AGTTGAAAAG 
AAAGAGATGG AGCAGCTGGA ATCTAAGAAG AAAGAGGCAT CTGCCTTAAG TCATTCTCTC 
CCAGATATCT CATGTTGGGA CAAAGTAGAG GAGGATGAAG CTGATCTGGT GGCGACAGAT 
GTTCAGTTGG ACCCCAATCT CCTTCCACTT GTGAATGGGG AAAATGATGA TCAAGTCTTA 
AGATTTTATT GGCTGGATGC TTATGAAGAT CAGTACAGTC AGCCAGGTGT GGTATTTTTG 
TTTGGCAAAG TTTGGATTGA ATCGGCGAAC ACCTATGTCA GCTGTTGTGT GACGGTGAAA 
AACATTGAGA GAACTGTTTA TCTACTGCCA CGTGAAACAG AGATGGATCT ATCCAGTGGG 
AAAGAGACAG AGACTCCAGT AACCATAATG AATGTTTTCA AGGAATTTAA TGACTGCATT 
TCACCAAAGT ATAAGATTAT GAAGTTCAAA TCCAAGAAAG TGGAAAAATA CTATGCGTTT 
GAGATCCCGG ATGTTCCAGC CAAGTCTGAA TACTTGGAAG TTAAATATTC AGCTGAATAC 
CCTCGGCTTC CTCAAGACCT GAAAGGAGAA ACGTTTTCAC ACGCGTTTGG AACTAACACT 
TCTAGCTTGG AGCTTCTCTT GATGAGTAGA AAGATCAAAG GACCGTGCTG GCTGGAAATC 
AAAAATCCAC AGCCCTCAAA TCAGTCAGTC AGCTGGTGTA AGGTAGAGGC AGTAGCCATG 
AAGCCTGGCT TGGTGAATGT GGTTAAAGAT CTTCCTCCTC CTCCTCCTCT TGTGGTAATG 
TCCTTCAGCA TGAAGACCAT TCAGAACCCA AAGACTCACC AGAATGAGAT TGTGGCTATG 
GCAGCTCTGA TTCATCAGAA ATTTCCTTTG GATAAAGCTC CACCTCAGCC TCCTTTTCAG 
ACACACTTCT GTGCTGTTTC CAAACCGAAT GATTGCATTT TTCCTTATGA CTTCAAAGAA 
GAGATTAAAA AGAAGAATGC TAAAATAGAA ATTGCTGCAA CAGAGAGAAC ACTGCTAGGA 
TTTTTCCTCG CGAAGATTCA CAAAATTGAC CCAGATGTTG TTGTGGGTCA CAATATTTAT 
GGATTTGATC TGGAAGTGCT GCTTCAAAGA ATTAATGTGT GCAAAGTCCC TCACTGGTCC 
AAGATAGGTC GACTGAGGAG GTCCAATATG CCAAAACTTG GGGGTCGAGG AGGATTTGGT 
GAAAGGAATG CTGCGTGTGG TCGGATGATC TGTGATGTAG AAATTTCAGC TAAAGAGTTA 
ATTCGCTGCA AGAGTTACCA TTTGTCTGAA CTGGTCCATC AGATTTTGAA AACAGAGAGG 
GTAACAATTC TACCAGAGGA AATTCGAAAT TTGTACAGCG ATTCTCCTCA CTTACTGTTC 
ATGCTGGAAA ACACCTGGAC AGATGCCAAG TTCATTCTTC AGATCATGTG CGAGCTGAAT 
GTTCTGCCAC TAGCCCTACA GATTACAAAC ATCTCTGGAA ATGTTATGTC GAGGACAATG 
ATGGGTGGAC GTTCTGAACG CAATGAGTTC TTACTGCTTC ATGCATTTTA TGAAAAGGAC 
TACATAGTCC CAGACAAACA AGTTTTTAAA AAGCCTCTAC AGAAGCTGGT GGATGAAGAT 
GAAGATTTTG AAGATCAGAA TAAATCAAAG ATAGGGAAAA AGAAAGCAGC ATATGCTGGG 
GGCTTAGTCT TGGATCCCAA AGTCGGTTTT TATGACAAGT TTATTTTGCT TCTCGACTTC 
AACAGCTTGT ACCCATCAAT TATCCAGGAG TTTAACATCT GCTTTACCAC AGTGCAGCGA 
CTGTCATCAG AAGCACAGAA AAGGGCAGAG GTGGAAGAAG AGGAAGAAAT TCCAGAGCTT 
CCGGACCCTT CTCTGGAAAT GGGAATTCTA CCTAAAGAAA TCAGGAAACT TGTGGAGAGA 
AGACGCCAAG TTAAGCAGTT AATGAAACAG CCAGACTTAA ATCCTGACCT TTATTTACAG 
TATGATATCA GACAGAAAGC TTTGAAACTC ACCGCTAACA GTATGTATGG CTGCCTAGGA 
TTTTCCTACA GCAGATTCTA TGCCAAGCCT TTGGCTGCTT TGGTGACGCA TAAAGGGAGA 
GAGATTTTGA TGCATACCAA GGAGATGGTG CAAAAGATGA ACCTTGAAGT GATTTATGGA 
GACACAGATT CCATTATGAT AAACACTAAC AGCACCAATT TGGATGAAGT CTTCAAGCTG 
GGGAACAAGA TCAAAAGTGA AGTGAACAAG TTGTATAAAT TGCTGGAGAT TGATATTGAT 
GGAGTCTTCA AGTCCTTGCT ACTGTTAAAG AAGAAGAAAT ATGCTGCCCT TACCGTGGAA 
CCAACAGGAG ATGGGAAATA TGTAACTAAA CAAGAACTGA AAGGGTTGGA TATAGTCCGA 
AGAGACTGGT GTGATCTTGC AAAAGAAACT GGAAACTATG TAATTGGTCA GATTCTTTCT 
GATCAGCCCC GAGACATAAT TGTGGAAAAT ATTCAAAGGC GGCTTATAGA AATTGGAGAA 
AATGTGATCA ATGGCCAGAT CCCAGTAAAT CAGTTTGAAA TAAATAAGGC ACTGACAAAA 
GATCCACAGG ATTATCCTGA CAAAAAAAGC CTGCCACATG TGCATGTTGC CATGTGGATA 
AACTCTCAAG GAGGCCGAAA GGTGAAGGCA GGAGATACAG TGTCATATAT CATTTGTCAG 
GATGGATCAA ATCTCAGTGC CAGCCAGCGA GCATATGCTC CAGAACAGTT GCAAAAGCAA 
GACAATCTTA CTATTGATAC TCAATACTAC TTGTCACAGC AAATACATCC AGTAGTTGCT 
AGAATATGTG AACCTATAGA GGGAATTGAT TCTGTTCTTA TCGCAACGTG GTTAGGACTG 
GATCCCTCCC AATTCAGAGT CCATCACCAT TACCATAAAG ATGAGGAGAA TGATGCCTTG 
GTTGGTGGCC CAGCTCAGCT CACTGATGAG GAGAAATATA GGGACTGTGA AAGATTCAAG 
TTTTGCTGCC TCAAGTGTGG AACAGAGAAT ATTTATGACA ATGTCTTTGA CTGTTCGGGG 
CGATTTATTG AGCCCAGCTT GCAAAGGTGC AGTAAAAGTG AATGTGAAGA GCCTCCTTTC 
AACCATGTGG TTCAAATGAA CAACAAATTA CTTTTGGATA TTAGACGCTA CCTAAAAAAA 
TACTATAATG GTTGGTTAAT ATGTGAGGAT CCAACTTGCC AGAATCGAAC CCGACGTCTT 
CCCCTCAGTT TTTCCCGGAG TGGTCCTATA TGCCAGGCCT GTAGGAAGGC TATCCTGAGA 
CCAGAGTATT CTGATAAGGC CCTGTACACT CAGCTGTGCT TTTACCGGTA CATTTTTGAT 
GTGGAGTATG CAACGGATAA AGTGATTACT GAAGAGGATA GAGAGTTCGT AAGGAGGAAG 
CCCCTCAGGA AGGAAGTGTC CGAGGCCTAC AGGAAACTGA AGAGCACGGT AGACAAATGC 
CTGTCCCTGA GCAGCTACTC GGAAGTGAAC CTCGGCAAAC TCTTCTCCGT CAGCATGGGA 
AGATCCGTGG GAGAGCCCAG CAGTGAAAGA CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLA1 ( XM_015302675.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015158161.1 Gallus gallus DNA polymerase alpha 1, catalytic subunit (POLA1), transcript variant X3, mRNA.

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CloneID	OGa30440
Clone ID Related Accession (Same CDS sequence)	XM_015302675.1 , XM_015302675.2
Accession Version	XM_015302675.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4476bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	DNA polymerase alpha catalytic subunit isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015302675.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGAGT CTGGGAGCTT TGCAACGTCA CGTTCTAGGC GTGAGAGGAA GTCAAAAAGA 
GGTCAGCAAG AAGCACTGGA ACGTCTGAAA AAGGCCAAAG CTGGGGAAAA ATTAAAATAC 
GAGGTTGAGG AGTTCACTGG TGTTTACGAT GAAATAGATG AAGAACAGTA CTCCAAGATA 
GTCAGAGAAC GTCAGGATGA TGACTGGATT GTTGATGATG ATGGGATAGG TTATGTAGAA 
GATGGACGGG AAATCTTTGA TGAAGATCTA GACGATGATG CTCTTGGTTC CAGTAAGAAA 
GGAAAAACCG GCAAAACTTC TACAATTGGT AAAAAGAATG TGAAAAAATC TGTGGTAGCA 
AAGCCAAACA CAATTAAATC TATGTTTATT GCCAGTGCTG GGAAGAAAAA CACGGACAAA 
ACTGTGGACC TGTCAAAGGA TGATTTACTA GGGGATATTC TTCAAGATCT AAATGCTGAA 
ACTGTTCAGC TAAGTCCACC ACCGATTTTA GTGCTGAAAA GGAAAAGATC TGCTGGAATG 
CCACTGAATC CTTTCTCTGT ACAGCGCCAG TCTCCTAAGG TAATCACCCC TGTATCAAAG 
AAAGCTCCTG TTCAACTCAG GAAAGAAGCT CGTCCTCCAG CTTCATTTCA TCCTAAACAT 
CCAAATTACA CAGCAAAATC TGTCACAGCC TCTGAAGAGA ATGAGGATAC CAAGTATGTG 
CAGAAAGCTA CAGAAAATGG GAGAGTAGTG AAGACAAAAG AAGAAAAAGC TATTGAAGAT 
GATGATCAGG GGATGATGGA TTTTGATGAT GAGGACTTTG ATGAACCTAT GGAAGCAGAG 
CTAATGGGAG CTGTACCTGA GACAGCTGAA ACCTTGAAAA GAGACAAGGA AGTTGAAAAG 
AAAGAGATGG AGCAGCTGGA ATCTAAGAAG AAAGAGGCAT CTGCCTTAAG TCATTCTCTC 
CCAGATATCT CATGTTGGGA CAAAGTAGAG GAGGATGAAG CTGATCTGGT GGCGACAGAT 
GTTCAGTTGG ACCCCAATCT CCTTCCACTT GTGAATGGGG AAAATGATGA TCAAGTCTTA 
AGATTTTATT GGCTGGATGC TTATGAAGAT CAGTACAGTC AGCCAGGTGT GGTATTTTTG 
TTTGGCAAAG TTTGGATTGA ATCGGCGAAC ACCTATGTCA GCTGTTGTGT GACGGTGAAA 
AACATTGAGA GAACTGTTTA TCTACTGCCA CGTGAAACAG AGATGGATCT ATCCAGTGGG 
AAAGAGACAG AGACTCCAGT AACCATAATG AATGTTTTCA AGGAATTTAA TGACTGCATT 
TCACCAAAGT ATAAGATTAT GAAGTTCAAA TCCAAGAAAG TGGAAAAATA CTATGCGTTT 
GAGATCCCGG ATGTTCCAGC CAAGTCTGAA TACTTGGAAG TTAAATATTC AGCTGAATAC 
CCTCGGCTTC CTCAAGACCT GAAAGGAGAA ACGTTTTCAC ACGCGTTTGG AACTAACACT 
TCTAGCTTGG AGCTTCTCTT GATGAGTAGA AAGATCAAAG GACCGTGCTG GCTGGAAATC 
AAAAATCCAC AGCCCTCAAA TCAGTCAGTC AGCTGGTGTA AGGTAGAGGC AGTAGCCATG 
AAGCCTGGCT TGGTGAATGT GGTTAAAGAT CTTCCTCCTC CTCCTCCTCT TGTGGTAATG 
TCCTTCAGCA TGAAGACCAT TCAGAACCCA AAGACTCACC AGAATGAGAT TGTGGCTATG 
GCAGCTCTGA TTCATCAGAA ATTTCCTTTG GATAAAGCTC CACCTCAGCC TCCTTTTCAG 
ACACACTTCT GTGCTGTTTC CAAACCGAAT GATTGCATTT TTCCTTATGA CTTCAAAGAA 
GAGATTAAAA AGAAGAATGC TAAAATAGAA ATTGCTGCAA CAGAGAGAAC ACTGCTAGGA 
TTTTTCCTCG CGAAGATTCA CAAAATTGAC CCAGATGTTG TTGTGGGTCA CAATATTTAT 
GGATTTGATC TGGAAGTGCT GCTTCAAAGA ATTAATGTGT GCAAAGTCCC TCACTGGTCC 
AAGATAGGTC GACTGAGGAG GTCCAATATG CCAAAACTTG GGGGTCGAGG AGGATTTGGT 
GAAAGGAATG CTGCGTGTGG TCGGATGATC TGTGATGTAG AAATTTCAGC TAAAGAGTTA 
ATTCGCTGCA AGAGTTACCA TTTGTCTGAA CTGGTCCATC AGATTTTGAA AACAGAGAGG 
GTAACAATTC TACCAGAGGA AATTCGAAAT TTGTACAGCG ATTCTCCTCA CTTACTGTTC 
ATGCTGGAAA ACACCTGGAC AGATGCCAAG TTCATTCTTC AGATCATGTG CGAGCTGAAT 
GTTCTGCCAC TAGCCCTACA GATTACAAAC ATCTCTGGAA ATGTTATGTC GAGGACAATG 
ATGGGTGGAC GTTCTGAACG CAATGAGTTC TTACTGCTTC ATGCATTTTA TGAAAAGGAC 
TACATAGTCC CAGACAAACA AGTTTTTAAA AAGCCTCTAC AGAAGCTGGT GGATGAAGAT 
GAAGATTTTG AAGATCAGAA TAAATCAAAG ATAGGGAAAA AGAAAGCAGC ATATGCTGGG 
GGCTTAGTCT TGGATCCCAA AGTCGGTTTT TATGACAAGT TTATTTTGCT TCTCGACTTC 
AACAGCTTGT ACCCATCAAT TATCCAGGAG TTTAACATCT GCTTTACCAC AGTGCAGCGA 
CTGTCATCAG AAGCACAGAA AAGGGCAGAG GTGGAAGAAG AGGAAGAAAT TCCAGAGCTT 
CCGGACCCTT CTCTGGAAAT GGGAATTCTA CCTAAAGAAA TCAGGAAACT TGTGGAGAGA 
AGACGCCAAG TTAAGCAGTT AATGAAACAG CCAGACTTAA ATCCTGACCT TTATTTACAG 
TATGATATCA GACAGAAAGC TTTGAAACTC ACCGCTAACA GTATGTATGG CTGCCTAGGA 
TTTTCCTACA GCAGATTCTA TGCCAAGCCT TTGGCTGCTT TGGTGACGCA TAAAGGGAGA 
GAGATTTTGA TGCATACCAA GGAGATGGTG CAAAAGATGA ACCTTGAAGT GATTTATGGA 
GACACAGATT CCATTATGAT AAACACTAAC AGCACCAATT TGGATGAAGT CTTCAAGCTG 
GGGAACAAGA TCAAAAGTGA AGTGAACAAG TTGTATAAAT TGCTGGAGAT TGATATTGAT 
GGAGTCTTCA AGTCCTTGCT ACTGTTAAAG AAGAAGAAAT ATGCTGCCCT TACCGTGGAA 
CCAACAGGAG ATGGGAAATA TGTAACTAAA CAAGAACTGA AAGGGTTGGA TATAGTCCGA 
AGAGACTGGT GTGATCTTGC AAAAGAAACT GGAAACTATG TAATTGGTCA GATTCTTTCT 
GATCAGCCCC GAGACATAAT TGTGGAAAAT ATTCAAAGGC GGCTTATAGA AATTGGAGAA 
AATGTGATCA ATGGCCAGAT CCCAGTAAAT CAGTTTGAAA TAAATAAGGC ACTGACAAAA 
GATCCACAGG ATTATCCTGA CAAAAAAAGC CTGCCACATG TGCATGTTGC CATGTGGATA 
AACTCTCAAG GAGGCCGAAA GGTGAAGGCA GGAGATACAG TGTCATATAT CATTTGTCAG 
GATGGATCAA ATCTCAGTGC CAGCCAGCGA GCATATGCTC CAGAACAGTT GCAAAAGCAA 
GACAATCTTA CTATTGATAC TCAATACTAC TTGTCACAGC AAATACATCC AGTAGTTGCT 
AGAATATGTG AACCTATAGA GGGAATTGAT TCTGTTCTTA TCGCAACGTG GTTAGGACTG 
GATCCCTCCC AATTCAGAGT CCATCACCAT TACCATAAAG ATGAGGAGAA TGATGCCTTG 
GTTGGTGGCC CAGCTCAGCT CACTGATGAG GAGAAATATA GGGACTGTGA AAGATTCAAG 
TTTTGCTGCC TCAAGTGTGG AACAGAGAAT ATTTATGACA ATGTCTTTGA CTGTTCGGGG 
CGATTTATTG AGCCCAGCTT GCAAAGGTGC AGTAAAAGTG AATGTGAAGA GCCTCCTTTC 
AACCATGTGG TTCAAATGAA CAACAAATTA CTTTTGGATA TTAGACGCTA CCTAAAAAAA 
TACTATAATG GTTGGTTAAT ATGTGAGGAT CCAACTTGCC AGAATCGAAC CCGACGTCTT 
CCCCTCAGTT TTTCCCGGAG TGGTCCTATA TGCCAGGCCT GTAGGAAGGC TATCCTGAGA 
CCAGAGTATT CTGATAAGGC CCTGTACACT CAGCTGTGCT TTTACCGGTA CATTTTTGAT 
GTGGAGTATG CAACGGATAA AGTGATTACT GAAGAGGATA GAGAGTTCGT AAGGAGGAAG 
CCCCTCAGGA AGGAAGTGTC CGAGGCCTAC AGGAAACTGA AGAGCACGGT AGACAAATGC 
CTGTCCCTGA GCAGCTACTC GGAAGTGAAC CTCGGCAAAC TCTTCTCCGT CAGCATGGGA 
AGATCCGTGG GAGAGCCCAG CAGTGAAAGA CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015158161.1
CDS	23..4498
Translation	MPESGSFATSRSRRERKSKRGQQEALERLKKAKAGEKLKYEVEEFTGVYDEIDEEQYSKIVRERQDDDWIVDDDGIGYVEDGREIFDEDLDDDALGSSKKGKTGKTSTIGKKNVKKSVVAKPNTIKSMFIASAGKKNTDKTVDLSKDDLLGDILQDLNAETVQLSPPPILVLKRKRSAGMPLNPFSVQRQSPKVITPVSKKAPVQLRKEARPPASFHPKHPNYTAKSVTASEENEDTKYVQKATENGRVVKTKEEKAIEDDDQGMMDFDDEDFDEPMEAELMGAVPETAETLKRDKEVEKKEMEQLESKKKEASALSHSLPDISCWDKVEEDEADLVATDVQLDPNLLPLVNGENDDQVLRFYWLDAYEDQYSQPGVVFLFGKVWIESANTYVSCCVTVKNIERTVYLLPRETEMDLSSGKETETPVTIMNVFKEFNDCISPKYKIMKFKSKKVEKYYAFEIPDVPAKSEYLEVKYSAEYPRLPQDLKGETFSHAFGTNTSSLELLLMSRKIKGPCWLEIKNPQPSNQSVSWCKVEAVAMKPGLVNVVKDLPPPPPLVVMSFSMKTIQNPKTHQNEIVAMAALIHQKFPLDKAPPQPPFQTHFCAVSKPNDCIFPYDFKEEIKKKNAKIEIAATERTLLGFFLAKIHKIDPDVVVGHNIYGFDLEVLLQRINVCKVPHWSKIGRLRRSNMPKLGGRGGFGERNAACGRMICDVEISAKELIRCKSYHLSELVHQILKTERVTILPEEIRNLYSDSPHLLFMLENTWTDAKFILQIMCELNVLPLALQITNISGNVMSRTMMGGRSERNEFLLLHAFYEKDYIVPDKQVFKKPLQKLVDEDEDFEDQNKSKIGKKKAAYAGGLVLDPKVGFYDKFILLLDFNSLYPSIIQEFNICFTTVQRLSSEAQKRAEVEEEEEIPELPDPSLEMGILPKEIRKLVERRRQVKQLMKQPDLNPDLYLQYDIRQKALKLTANSMYGCLGFSYSRFYAKPLAALVTHKGREILMHTKEMVQKMNLEVIYGDTDSIMINTNSTNLDEVFKLGNKIKSEVNKLYKLLEIDIDGVFKSLLLLKKKKYAALTVEPTGDGKYVTKQELKGLDIVRRDWCDLAKETGNYVIGQILSDQPRDIIVENIQRRLIEIGENVINGQIPVNQFEINKALTKDPQDYPDKKSLPHVHVAMWINSQGGRKVKAGDTVSYIICQDGSNLSASQRAYAPEQLQKQDNLTIDTQYYLSQQIHPVVARICEPIEGIDSVLIATWLGLDPSQFRVHHHYHKDEENDALVGGPAQLTDEEKYRDCERFKFCCLKCGTENIYDNVFDCSGRFIEPSLQRCSKSECEEPPFNHVVQMNNKLLLDIRRYLKKYYNGWLICEDPTCQNRTRRLPLSFSRSGPICQACRKAILRPEYSDKALYTQLCFYRYIFDVEYATDKVITEEDREFVRRKPLRKEVSEAYRKLKSTVDKCLSLSSYSEVNLGKLFSVSMGRSVGEPSSERQ

Target ORF information:

RefSeq Version	XM_015302675.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus DNA polymerase alpha 1, catalytic subunit (POLA1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015302675.2

ORF Insert Sequence:

ATGCCTGAGT CTGGGAGCTT TGCAACGTCA CGTTCTAGGC GTGAGAGGAA GTCAAAAAGA 
GGTCAGCAAG AAGCACTGGA ACGTCTGAAA AAGGCCAAAG CTGGGGAAAA ATTAAAATAC 
GAGGTTGAGG AGTTCACTGG TGTTTACGAT GAAATAGATG AAGAACAGTA CTCCAAGATA 
GTCAGAGAAC GTCAGGATGA TGACTGGATT GTTGATGATG ATGGGATAGG TTATGTAGAA 
GATGGACGGG AAATCTTTGA TGAAGATCTA GACGATGATG CTCTTGGTTC CAGTAAGAAA 
GGAAAAACCG GCAAAACTTC TACAATTGGT AAAAAGAATG TGAAAAAATC TGTGGTAGCA 
AAGCCAAACA CAATTAAATC TATGTTTATT GCCAGTGCTG GGAAGAAAAA CACGGACAAA 
ACTGTGGACC TGTCAAAGGA TGATTTACTA GGGGATATTC TTCAAGATCT AAATGCTGAA 
ACTGTTCAGC TAAGTCCACC ACCGATTTTA GTGCTGAAAA GGAAAAGATC TGCTGGAATG 
CCACTGAATC CTTTCTCTGT ACAGCGCCAG TCTCCTAAGG TAATCACCCC TGTATCAAAG 
AAAGCTCCTG TTCAACTCAG GAAAGAAGCT CGTCCTCCAG CTTCATTTCA TCCTAAACAT 
CCAAATTACA CAGCAAAATC TGTCACAGCC TCTGAAGAGA ATGAGGATAC CAAGTATGTG 
CAGAAAGCTA CAGAAAATGG GAGAGTAGTG AAGACAAAAG AAGAAAAAGC TATTGAAGAT 
GATGATCAGG GGATGATGGA TTTTGATGAT GAGGACTTTG ATGAACCTAT GGAAGCAGAG 
CTAATGGGAG CTGTACCTGA GACAGCTGAA ACCTTGAAAA GAGACAAGGA AGTTGAAAAG 
AAAGAGATGG AGCAGCTGGA ATCTAAGAAG AAAGAGGCAT CTGCCTTAAG TCATTCTCTC 
CCAGATATCT CATGTTGGGA CAAAGTAGAG GAGGATGAAG CTGATCTGGT GGCGACAGAT 
GTTCAGTTGG ACCCCAATCT CCTTCCACTT GTGAATGGGG AAAATGATGA TCAAGTCTTA 
AGATTTTATT GGCTGGATGC TTATGAAGAT CAGTACAGTC AGCCAGGTGT GGTATTTTTG 
TTTGGCAAAG TTTGGATTGA ATCGGCGAAC ACCTATGTCA GCTGTTGTGT GACGGTGAAA 
AACATTGAGA GAACTGTTTA TCTACTGCCA CGTGAAACAG AGATGGATCT ATCCAGTGGG 
AAAGAGACAG AGACTCCAGT AACCATAATG AATGTTTTCA AGGAATTTAA TGACTGCATT 
TCACCAAAGT ATAAGATTAT GAAGTTCAAA TCCAAGAAAG TGGAAAAATA CTATGCGTTT 
GAGATCCCGG ATGTTCCAGC CAAGTCTGAA TACTTGGAAG TTAAATATTC AGCTGAATAC 
CCTCGGCTTC CTCAAGACCT GAAAGGAGAA ACGTTTTCAC ACGCGTTTGG AACTAACACT 
TCTAGCTTGG AGCTTCTCTT GATGAGTAGA AAGATCAAAG GACCGTGCTG GCTGGAAATC 
AAAAATCCAC AGCCCTCAAA TCAGTCAGTC AGCTGGTGTA AGGTAGAGGC AGTAGCCATG 
AAGCCTGGCT TGGTGAATGT GGTTAAAGAT CTTCCTCCTC CTCCTCCTCT TGTGGTAATG 
TCCTTCAGCA TGAAGACCAT TCAGAACCCA AAGACTCACC AGAATGAGAT TGTGGCTATG 
GCAGCTCTGA TTCATCAGAA ATTTCCTTTG GATAAAGCTC CACCTCAGCC TCCTTTTCAG 
ACACACTTCT GTGCTGTTTC CAAACCGAAT GATTGCATTT TTCCTTATGA CTTCAAAGAA 
GAGATTAAAA AGAAGAATGC TAAAATAGAA ATTGCTGCAA CAGAGAGAAC ACTGCTAGGA 
TTTTTCCTCG CGAAGATTCA CAAAATTGAC CCAGATGTTG TTGTGGGTCA CAATATTTAT 
GGATTTGATC TGGAAGTGCT GCTTCAAAGA ATTAATGTGT GCAAAGTCCC TCACTGGTCC 
AAGATAGGTC GACTGAGGAG GTCCAATATG CCAAAACTTG GGGGTCGAGG AGGATTTGGT 
GAAAGGAATG CTGCGTGTGG TCGGATGATC TGTGATGTAG AAATTTCAGC TAAAGAGTTA 
ATTCGCTGCA AGAGTTACCA TTTGTCTGAA CTGGTCCATC AGATTTTGAA AACAGAGAGG 
GTAACAATTC TACCAGAGGA AATTCGAAAT TTGTACAGCG ATTCTCCTCA CTTACTGTTC 
ATGCTGGAAA ACACCTGGAC AGATGCCAAG TTCATTCTTC AGATCATGTG CGAGCTGAAT 
GTTCTGCCAC TAGCCCTACA GATTACAAAC ATCTCTGGAA ATGTTATGTC GAGGACAATG 
ATGGGTGGAC GTTCTGAACG CAATGAGTTC TTACTGCTTC ATGCATTTTA TGAAAAGGAC 
TACATAGTCC CAGACAAACA AGTTTTTAAA AAGCCTCTAC AGAAGCTGGT GGATGAAGAT 
GAAGATTTTG AAGATCAGAA TAAATCAAAG ATAGGGAAAA AGAAAGCAGC ATATGCTGGG 
GGCTTAGTCT TGGATCCCAA AGTCGGTTTT TATGACAAGT TTATTTTGCT TCTCGACTTC 
AACAGCTTGT ACCCATCAAT TATCCAGGAG TTTAACATCT GCTTTACCAC AGTGCAGCGA 
CTGTCATCAG AAGCACAGAA AAGGGCAGAG GTGGAAGAAG AGGAAGAAAT TCCAGAGCTT 
CCGGACCCTT CTCTGGAAAT GGGAATTCTA CCTAAAGAAA TCAGGAAACT TGTGGAGAGA 
AGACGCCAAG TTAAGCAGTT AATGAAACAG CCAGACTTAA ATCCTGACCT TTATTTACAG 
TATGATATCA GACAGAAAGC TTTGAAACTC ACCGCTAACA GTATGTATGG CTGCCTAGGA 
TTTTCCTACA GCAGATTCTA TGCCAAGCCT TTGGCTGCTT TGGTGACGCA TAAAGGGAGA 
GAGATTTTGA TGCATACCAA GGAGATGGTG CAAAAGATGA ACCTTGAAGT GATTTATGGA 
GACACAGATT CCATTATGAT AAACACTAAC AGCACCAATT TGGATGAAGT CTTCAAGCTG 
GGGAACAAGA TCAAAAGTGA AGTGAACAAG TTGTATAAAT TGCTGGAGAT TGATATTGAT 
GGAGTCTTCA AGTCCTTGCT ACTGTTAAAG AAGAAGAAAT ATGCTGCCCT TACCGTGGAA 
CCAACAGGAG ATGGGAAATA TGTAACTAAA CAAGAACTGA AAGGGTTGGA TATAGTCCGA 
AGAGACTGGT GTGATCTTGC AAAAGAAACT GGAAACTATG TAATTGGTCA GATTCTTTCT 
GATCAGCCCC GAGACATAAT TGTGGAAAAT ATTCAAAGGC GGCTTATAGA AATTGGAGAA 
AATGTGATCA ATGGCCAGAT CCCAGTAAAT CAGTTTGAAA TAAATAAGGC ACTGACAAAA 
GATCCACAGG ATTATCCTGA CAAAAAAAGC CTGCCACATG TGCATGTTGC CATGTGGATA 
AACTCTCAAG GAGGCCGAAA GGTGAAGGCA GGAGATACAG TGTCATATAT CATTTGTCAG 
GATGGATCAA ATCTCAGTGC CAGCCAGCGA GCATATGCTC CAGAACAGTT GCAAAAGCAA 
GACAATCTTA CTATTGATAC TCAATACTAC TTGTCACAGC AAATACATCC AGTAGTTGCT 
AGAATATGTG AACCTATAGA GGGAATTGAT TCTGTTCTTA TCGCAACGTG GTTAGGACTG 
GATCCCTCCC AATTCAGAGT CCATCACCAT TACCATAAAG ATGAGGAGAA TGATGCCTTG 
GTTGGTGGCC CAGCTCAGCT CACTGATGAG GAGAAATATA GGGACTGTGA AAGATTCAAG 
TTTTGCTGCC TCAAGTGTGG AACAGAGAAT ATTTATGACA ATGTCTTTGA CTGTTCGGGG 
CGATTTATTG AGCCCAGCTT GCAAAGGTGC AGTAAAAGTG AATGTGAAGA GCCTCCTTTC 
AACCATGTGG TTCAAATGAA CAACAAATTA CTTTTGGATA TTAGACGCTA CCTAAAAAAA 
TACTATAATG GTTGGTTAAT ATGTGAGGAT CCAACTTGCC AGAATCGAAC CCGACGTCTT 
CCCCTCAGTT TTTCCCGGAG TGGTCCTATA TGCCAGGCCT GTAGGAAGGC TATCCTGAGA 
CCAGAGTATT CTGATAAGGC CCTGTACACT CAGCTGTGCT TTTACCGGTA CATTTTTGAT 
GTGGAGTATG CAACGGATAA AGTGATTACT GAAGAGGATA GAGAGTTCGT AAGGAGGAAG 
CCCCTCAGGA AGGAAGTGTC CGAGGCCTAC AGGAAACTGA AGAGCACGGT AGACAAATGC 
CTGTCCCTGA GCAGCTACTC GGAAGTGAAC CTCGGCAAAC TCTTCTCCGT CAGCATGGGA 
AGATCCGTGG GAGAGCCCAG CAGTGAAAGA CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLA1 ( XM_025146469.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025002237.1 Gallus gallus DNA polymerase alpha 1, catalytic subunit (POLA1), transcript variant X1, mRNA.

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CloneID	OGa142989
Clone ID Related Accession (Same CDS sequence)	XM_025146469.1
Accession Version	XM_025146469.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4572bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	DNA polymerase alpha catalytic subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTTG AGGGGTGCTT TGGAAGTCCG GGAGGCAGCT TCTTTTCCCT CCCCATCCTG 
CTGGACATCG GCTGCCATGG AACCTGCTTG CACTCGATGC CTGAGTCTGG GAGCTTTGCA 
ACGTCACGTT CTAGGCGTGA GAGGAAGTCA AAAAGAGGTC AGCAAGAAGC ACTGGAACGT 
CTGAAAAAGG CCAAAGCTGG GGAAAAATTA AAATACGAGG TTGAGGAGTT CACTGGTGTT 
TACGATGAAA TAGATGAAGA ACAGTACTCC AAGATAGTCA GAGAACGTCA GGATGATGAC 
TGGATTGTTG ATGATGATGG GATAGGTTAT GTAGAAGATG GACGGGAAAT CTTTGATGAA 
GATCTAGACG ATGATGCTCT TGGTTCCAGT AAGAAAGGAA AAACCGGCAA AACTTCTACA 
ATTGGTAAAA AGAATGTGAA AAAATCTGTG GTAGCAAAGC CAAACACAAT TAAATCTATG 
TTTATTGCCA GTGCTGGGAA GAAAAACACG GACAAAACTG TGGACCTGTC AAAGGATGAT 
TTACTAGGGG ATATTCTTCA AGATCTAAAT GCTGAAACTG TTCAGCTAAG TCCACCACCG 
ATTTTAGTGC TGAAAAGGAA AAGATCTGCT GGAATGCCAC TGAATCCTTT CTCTGTACAG 
CGCCAGTCTC CTAAGGTAAT CACCCCTGTA TCAAAGAAAG CTCCTGTTCA ACTCAGGAAA 
GAAGCTCGTC CTCCAGCTTC ATTTCATCCT AAACATCCAA ATTACACAGC AAAATCTGTC 
ACAGCCTCTG AAGAGAATGA GGATACCAAG TATGTGCAGA AAGCTACAGA AAATGGGAGA 
GTAGTGAAGA CAAAAGAAGA AAAAGCTATT GAAGATGATG ATCAGGGGAT GATGGATTTT 
GATGATGAGG ACTTTGATGA ACCTATGGAA GCAGAGCTAA TGGGAGCTGT ACCTGAGACA 
GCTGAAACCT TGAAAAGAGA CAAGGAAGTT GAAAAGAAAG AGATGGAGCA GCTGGAATCT 
AAGAAGAAAG AGGCATCTGC CTTAAGTCAT TCTCTCCCAG ATATCTCATG TTGGGACAAA 
GTAGAGGAGG ATGAAGCTGA TCTGGTGGCG ACAGATGTTC AGTTGGACCC CAATCTCCTT 
CCACTTGTGA ATGGGGAAAA TGATGATCAA GTCTTAAGAT TTTATTGGCT GGATGCTTAT 
GAAGATCAGT ACAGTCAGCC AGGTGTGGTA TTTTTGTTTG GCAAAGTTTG GATTGAATCG 
GCGAACACCT ATGTCAGCTG TTGTGTGACG GTGAAAAACA TTGAGAGAAC TGTTTATCTA 
CTGCCACGTG AAACAGAGAT GGATCTATCC AGTGGGAAAG AGACAGAGAC TCCAGTAACC 
ATAATGAATG TTTTCAAGGA ATTTAATGAC TGCATTTCAC CAAAGTATAA GATTATGAAG 
TTCAAATCCA AGAAAGTGGA AAAATACTAT GCGTTTGAGA TCCCGGATGT TCCAGCCAAG 
TCTGAATACT TGGAAGTTAA ATATTCAGCT GAATACCCTC GGCTTCCTCA AGACCTGAAA 
GGAGAAACGT TTTCACACGC GTTTGGAACT AACACTTCTA GCTTGGAGCT TCTCTTGATG 
AGTAGAAAGA TCAAAGGACC GTGCTGGCTG GAAATCAAAA ATCCACAGCC CTCAAATCAG 
TCAGTCAGCT GGTGTAAGGT AGAGGCAGTA GCCATGAAGC CTGGCTTGGT GAATGTGGTT 
AAAGATCTTC CTCCTCCTCC TCCTCTTGTG GTAATGTCCT TCAGCATGAA GACCATTCAG 
AACCCAAAGA CTCACCAGAA TGAGATTGTG GCTATGGCAG CTCTGATTCA TCAGAAATTT 
CCTTTGGATA AAGCTCCACC TCAGCCTCCT TTTCAGACAC ACTTCTGTGC TGTTTCCAAA 
CCGAATGATT GCATTTTTCC TTATGACTTC AAAGAAGAGA TTAAAAAGAA GAATGCTAAA 
ATAGAAATTG CTGCAACAGA GAGAACACTG CTAGGATTTT TCCTCGCGAA GATTCACAAA 
ATTGACCCAG ATGTTGTTGT GGGTCACAAT ATTTATGGAT TTGATCTGGA AGTGCTGCTT 
CAAAGAATTA ATGTGTGCAA AGTCCCTCAC TGGTCCAAGA TAGGTCGACT GAGGAGGTCC 
AATATGCCAA AACTTGGGGG TCGAGGAGGA TTTGGTGAAA GGAATGCTGC GTGTGGTCGG 
ATGATCTGTG ATGTAGAAAT TTCAGCTAAA GAGTTAATTC GCTGCAAGAG TTACCATTTG 
TCTGAACTGG TCCATCAGAT TTTGAAAACA GAGAGGGTAA CAATTCTACC AGAGGAAATT 
CGAAATTTGT ACAGCGATTC TCCTCACTTA CTGTTCATGC TGGAAAACAC CTGGACAGAT 
GCCAAGTTCA TTCTTCAGAT CATGTGCGAG CTGAATGTTC TGCCACTAGC CCTACAGATT 
ACAAACATCT CTGGAAATGT TATGTCGAGG ACAATGATGG GTGGACGTTC TGAACGCAAT 
GAGTTCTTAC TGCTTCATGC ATTTTATGAA AAGGACTACA TAGTCCCAGA CAAACAAGTT 
TTTAAAAAGC CTCTACAGAA GCTGGTGGAT GAAGATGAAG ATTTTGAAGA TCAGAATAAA 
TCAAAGATAG GGAAAAAGAA AGCAGCATAT GCTGGGGGCT TAGTCTTGGA TCCCAAAGTC 
GGTTTTTATG ACAAGTTTAT TTTGCTTCTC GACTTCAACA GCTTGTACCC ATCAATTATC 
CAGGAGTTTA ACATCTGCTT TACCACAGTG CAGCGACTGT CATCAGAAGC ACAGAAAAGG 
GCAGAGGTGG AAGAAGAGGA AGAAATTCCA GAGCTTCCGG ACCCTTCTCT GGAAATGGGA 
ATTCTACCTA AAGAAATCAG GAAACTTGTG GAGAGAAGAC GCCAAGTTAA GCAGTTAATG 
AAACAGCCAG ACTTAAATCC TGACCTTTAT TTACAGTATG ATATCAGACA GAAAGCTTTG 
AAACTCACCG CTAACAGTAT GTATGGCTGC CTAGGATTTT CCTACAGCAG ATTCTATGCC 
AAGCCTTTGG CTGCTTTGGT GACGCATAAA GGGAGAGAGA TTTTGATGCA TACCAAGGAG 
ATGGTGCAAA AGATGAACCT TGAAGTGATT TATGGAGACA CAGATTCCAT TATGATAAAC 
ACTAACAGCA CCAATTTGGA TGAAGTCTTC AAGCTGGGGA ACAAGATCAA AAGTGAAGTG 
AACAAGTTGT ATAAATTGCT GGAGATTGAT ATTGATGGAG TCTTCAAGTC CTTGCTACTG 
TTAAAGAAGA AGAAATATGC TGCCCTTACC GTGGAACCAA CAGGAGATGG GAAATATGTA 
ACTAAACAAG AACTGAAAGG GTTGGATATA GTCCGAAGAG ACTGGTGTGA TCTTGCAAAA 
GAAACTGGAA ACTATGTAAT TGGTCAGATT CTTTCTGATC AGCCCCGAGA CATAATTGTG 
GAAAATATTC AAAGGCGGCT TATAGAAATT GGAGAAAATG TGATCAATGG CCAGATCCCA 
GTAAATCAGT TTGAAATAAA TAAGGCACTG ACAAAAGATC CACAGGATTA TCCTGACAAA 
AAAAGCCTGC CACATGTGCA TGTTGCCATG TGGATAAACT CTCAAGGAGG CCGAAAGGTG 
AAGGCAGGAG ATACAGTGTC ATATATCATT TGTCAGGATG GATCAAATCT CAGTGCCAGC 
CAGCGAGCAT ATGCTCCAGA ACAGTTGCAA AAGCAAGACA ATCTTACTAT TGATACTCAA 
TACTACTTGT CACAGCAAAT ACATCCAGTA GTTGCTAGAA TATGTGAACC TATAGAGGGA 
ATTGATTCTG TTCTTATCGC AACGTGGTTA GGACTGGATC CCTCCCAATT CAGAGTCCAT 
CACCATTACC ATAAAGATGA GGAGAATGAT GCCTTGGTTG GTGGCCCAGC TCAGCTCACT 
GATGAGGAGA AATATAGGGA CTGTGAAAGA TTCAAGTTTT GCTGCCTCAA GTGTGGAACA 
GAGAATATTT ATGACAATGT CTTTGACTGT TCGGGGCGAT TTATTGAGCC CAGCTTGCAA 
AGGTGCAGTA AAAGTGAATG TGAAGAGCCT CCTTTCAACC ATGTGGTTCA AATGAACAAC 
AAATTACTTT TGGATATTAG ACGCTACCTA AAAAAATACT ATAATGGTTG GTTAATATGT 
GAGGATCCAA CTTGCCAGAA TCGAACCCGA CGTCTTCCCC TCAGTTTTTC CCGGAGTGGT 
CCTATATGCC AGGCCTGTAG GAAGGCTATC CTGAGACCAG AGTATTCTGA TAAGGCCCTG 
TACACTCAGC TGTGCTTTTA CCGGTACATT TTTGATGTGG AGTATGCAAC GGATAAAGTG 
ATTACTGAAG AGGATAGAGA GTTCGTAAGG AGGAAGCCCC TCAGGAAGGA AGTGTCCGAG 
GCCTACAGGA AACTGAAGAG CACGGTAGAC AAATGCCTGT CCCTGAGCAG CTACTCGGAA 
GTGAACCTCG GCAAACTCTT CTCCGTCAGC ATGGGAAGAT CCGTGGGAGA GCCCAGCAGT 
GAAAGACAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025002237.1
CDS	16..4587
Translation	MRFEGCFGSPGGSFFSLPILLDIGCHGTCLHSMPESGSFATSRSRRERKSKRGQQEALERLKKAKAGEKLKYEVEEFTGVYDEIDEEQYSKIVRERQDDDWIVDDDGIGYVEDGREIFDEDLDDDALGSSKKGKTGKTSTIGKKNVKKSVVAKPNTIKSMFIASAGKKNTDKTVDLSKDDLLGDILQDLNAETVQLSPPPILVLKRKRSAGMPLNPFSVQRQSPKVITPVSKKAPVQLRKEARPPASFHPKHPNYTAKSVTASEENEDTKYVQKATENGRVVKTKEEKAIEDDDQGMMDFDDEDFDEPMEAELMGAVPETAETLKRDKEVEKKEMEQLESKKKEASALSHSLPDISCWDKVEEDEADLVATDVQLDPNLLPLVNGENDDQVLRFYWLDAYEDQYSQPGVVFLFGKVWIESANTYVSCCVTVKNIERTVYLLPRETEMDLSSGKETETPVTIMNVFKEFNDCISPKYKIMKFKSKKVEKYYAFEIPDVPAKSEYLEVKYSAEYPRLPQDLKGETFSHAFGTNTSSLELLLMSRKIKGPCWLEIKNPQPSNQSVSWCKVEAVAMKPGLVNVVKDLPPPPPLVVMSFSMKTIQNPKTHQNEIVAMAALIHQKFPLDKAPPQPPFQTHFCAVSKPNDCIFPYDFKEEIKKKNAKIEIAATERTLLGFFLAKIHKIDPDVVVGHNIYGFDLEVLLQRINVCKVPHWSKIGRLRRSNMPKLGGRGGFGERNAACGRMICDVEISAKELIRCKSYHLSELVHQILKTERVTILPEEIRNLYSDSPHLLFMLENTWTDAKFILQIMCELNVLPLALQITNISGNVMSRTMMGGRSERNEFLLLHAFYEKDYIVPDKQVFKKPLQKLVDEDEDFEDQNKSKIGKKKAAYAGGLVLDPKVGFYDKFILLLDFNSLYPSIIQEFNICFTTVQRLSSEAQKRAEVEEEEEIPELPDPSLEMGILPKEIRKLVERRRQVKQLMKQPDLNPDLYLQYDIRQKALKLTANSMYGCLGFSYSRFYAKPLAALVTHKGREILMHTKEMVQKMNLEVIYGDTDSIMINTNSTNLDEVFKLGNKIKSEVNKLYKLLEIDIDGVFKSLLLLKKKKYAALTVEPTGDGKYVTKQELKGLDIVRRDWCDLAKETGNYVIGQILSDQPRDIIVENIQRRLIEIGENVINGQIPVNQFEINKALTKDPQDYPDKKSLPHVHVAMWINSQGGRKVKAGDTVSYIICQDGSNLSASQRAYAPEQLQKQDNLTIDTQYYLSQQIHPVVARICEPIEGIDSVLIATWLGLDPSQFRVHHHYHKDEENDALVGGPAQLTDEEKYRDCERFKFCCLKCGTENIYDNVFDCSGRFIEPSLQRCSKSECEEPPFNHVVQMNNKLLLDIRRYLKKYYNGWLICEDPTCQNRTRRLPLSFSRSGPICQACRKAILRPEYSDKALYTQLCFYRYIFDVEYATDKVITEEDREFVRRKPLRKEVSEAYRKLKSTVDKCLSLSSYSEVNLGKLFSVSMGRSVGEPSSERQ

Target ORF information:

RefSeq Version	XM_025146469.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus DNA polymerase alpha 1, catalytic subunit (POLA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025146469.1

ORF Insert Sequence:

ATGAGATTTG AGGGGTGCTT TGGAAGTCCG GGAGGCAGCT TCTTTTCCCT CCCCATCCTG 
CTGGACATCG GCTGCCATGG AACCTGCTTG CACTCGATGC CTGAGTCTGG GAGCTTTGCA 
ACGTCACGTT CTAGGCGTGA GAGGAAGTCA AAAAGAGGTC AGCAAGAAGC ACTGGAACGT 
CTGAAAAAGG CCAAAGCTGG GGAAAAATTA AAATACGAGG TTGAGGAGTT CACTGGTGTT 
TACGATGAAA TAGATGAAGA ACAGTACTCC AAGATAGTCA GAGAACGTCA GGATGATGAC 
TGGATTGTTG ATGATGATGG GATAGGTTAT GTAGAAGATG GACGGGAAAT CTTTGATGAA 
GATCTAGACG ATGATGCTCT TGGTTCCAGT AAGAAAGGAA AAACCGGCAA AACTTCTACA 
ATTGGTAAAA AGAATGTGAA AAAATCTGTG GTAGCAAAGC CAAACACAAT TAAATCTATG 
TTTATTGCCA GTGCTGGGAA GAAAAACACG GACAAAACTG TGGACCTGTC AAAGGATGAT 
TTACTAGGGG ATATTCTTCA AGATCTAAAT GCTGAAACTG TTCAGCTAAG TCCACCACCG 
ATTTTAGTGC TGAAAAGGAA AAGATCTGCT GGAATGCCAC TGAATCCTTT CTCTGTACAG 
CGCCAGTCTC CTAAGGTAAT CACCCCTGTA TCAAAGAAAG CTCCTGTTCA ACTCAGGAAA 
GAAGCTCGTC CTCCAGCTTC ATTTCATCCT AAACATCCAA ATTACACAGC AAAATCTGTC 
ACAGCCTCTG AAGAGAATGA GGATACCAAG TATGTGCAGA AAGCTACAGA AAATGGGAGA 
GTAGTGAAGA CAAAAGAAGA AAAAGCTATT GAAGATGATG ATCAGGGGAT GATGGATTTT 
GATGATGAGG ACTTTGATGA ACCTATGGAA GCAGAGCTAA TGGGAGCTGT ACCTGAGACA 
GCTGAAACCT TGAAAAGAGA CAAGGAAGTT GAAAAGAAAG AGATGGAGCA GCTGGAATCT 
AAGAAGAAAG AGGCATCTGC CTTAAGTCAT TCTCTCCCAG ATATCTCATG TTGGGACAAA 
GTAGAGGAGG ATGAAGCTGA TCTGGTGGCG ACAGATGTTC AGTTGGACCC CAATCTCCTT 
CCACTTGTGA ATGGGGAAAA TGATGATCAA GTCTTAAGAT TTTATTGGCT GGATGCTTAT 
GAAGATCAGT ACAGTCAGCC AGGTGTGGTA TTTTTGTTTG GCAAAGTTTG GATTGAATCG 
GCGAACACCT ATGTCAGCTG TTGTGTGACG GTGAAAAACA TTGAGAGAAC TGTTTATCTA 
CTGCCACGTG AAACAGAGAT GGATCTATCC AGTGGGAAAG AGACAGAGAC TCCAGTAACC 
ATAATGAATG TTTTCAAGGA ATTTAATGAC TGCATTTCAC CAAAGTATAA GATTATGAAG 
TTCAAATCCA AGAAAGTGGA AAAATACTAT GCGTTTGAGA TCCCGGATGT TCCAGCCAAG 
TCTGAATACT TGGAAGTTAA ATATTCAGCT GAATACCCTC GGCTTCCTCA AGACCTGAAA 
GGAGAAACGT TTTCACACGC GTTTGGAACT AACACTTCTA GCTTGGAGCT TCTCTTGATG 
AGTAGAAAGA TCAAAGGACC GTGCTGGCTG GAAATCAAAA ATCCACAGCC CTCAAATCAG 
TCAGTCAGCT GGTGTAAGGT AGAGGCAGTA GCCATGAAGC CTGGCTTGGT GAATGTGGTT 
AAAGATCTTC CTCCTCCTCC TCCTCTTGTG GTAATGTCCT TCAGCATGAA GACCATTCAG 
AACCCAAAGA CTCACCAGAA TGAGATTGTG GCTATGGCAG CTCTGATTCA TCAGAAATTT 
CCTTTGGATA AAGCTCCACC TCAGCCTCCT TTTCAGACAC ACTTCTGTGC TGTTTCCAAA 
CCGAATGATT GCATTTTTCC TTATGACTTC AAAGAAGAGA TTAAAAAGAA GAATGCTAAA 
ATAGAAATTG CTGCAACAGA GAGAACACTG CTAGGATTTT TCCTCGCGAA GATTCACAAA 
ATTGACCCAG ATGTTGTTGT GGGTCACAAT ATTTATGGAT TTGATCTGGA AGTGCTGCTT 
CAAAGAATTA ATGTGTGCAA AGTCCCTCAC TGGTCCAAGA TAGGTCGACT GAGGAGGTCC 
AATATGCCAA AACTTGGGGG TCGAGGAGGA TTTGGTGAAA GGAATGCTGC GTGTGGTCGG 
ATGATCTGTG ATGTAGAAAT TTCAGCTAAA GAGTTAATTC GCTGCAAGAG TTACCATTTG 
TCTGAACTGG TCCATCAGAT TTTGAAAACA GAGAGGGTAA CAATTCTACC AGAGGAAATT 
CGAAATTTGT ACAGCGATTC TCCTCACTTA CTGTTCATGC TGGAAAACAC CTGGACAGAT 
GCCAAGTTCA TTCTTCAGAT CATGTGCGAG CTGAATGTTC TGCCACTAGC CCTACAGATT 
ACAAACATCT CTGGAAATGT TATGTCGAGG ACAATGATGG GTGGACGTTC TGAACGCAAT 
GAGTTCTTAC TGCTTCATGC ATTTTATGAA AAGGACTACA TAGTCCCAGA CAAACAAGTT 
TTTAAAAAGC CTCTACAGAA GCTGGTGGAT GAAGATGAAG ATTTTGAAGA TCAGAATAAA 
TCAAAGATAG GGAAAAAGAA AGCAGCATAT GCTGGGGGCT TAGTCTTGGA TCCCAAAGTC 
GGTTTTTATG ACAAGTTTAT TTTGCTTCTC GACTTCAACA GCTTGTACCC ATCAATTATC 
CAGGAGTTTA ACATCTGCTT TACCACAGTG CAGCGACTGT CATCAGAAGC ACAGAAAAGG 
GCAGAGGTGG AAGAAGAGGA AGAAATTCCA GAGCTTCCGG ACCCTTCTCT GGAAATGGGA 
ATTCTACCTA AAGAAATCAG GAAACTTGTG GAGAGAAGAC GCCAAGTTAA GCAGTTAATG 
AAACAGCCAG ACTTAAATCC TGACCTTTAT TTACAGTATG ATATCAGACA GAAAGCTTTG 
AAACTCACCG CTAACAGTAT GTATGGCTGC CTAGGATTTT CCTACAGCAG ATTCTATGCC 
AAGCCTTTGG CTGCTTTGGT GACGCATAAA GGGAGAGAGA TTTTGATGCA TACCAAGGAG 
ATGGTGCAAA AGATGAACCT TGAAGTGATT TATGGAGACA CAGATTCCAT TATGATAAAC 
ACTAACAGCA CCAATTTGGA TGAAGTCTTC AAGCTGGGGA ACAAGATCAA AAGTGAAGTG 
AACAAGTTGT ATAAATTGCT GGAGATTGAT ATTGATGGAG TCTTCAAGTC CTTGCTACTG 
TTAAAGAAGA AGAAATATGC TGCCCTTACC GTGGAACCAA CAGGAGATGG GAAATATGTA 
ACTAAACAAG AACTGAAAGG GTTGGATATA GTCCGAAGAG ACTGGTGTGA TCTTGCAAAA 
GAAACTGGAA ACTATGTAAT TGGTCAGATT CTTTCTGATC AGCCCCGAGA CATAATTGTG 
GAAAATATTC AAAGGCGGCT TATAGAAATT GGAGAAAATG TGATCAATGG CCAGATCCCA 
GTAAATCAGT TTGAAATAAA TAAGGCACTG ACAAAAGATC CACAGGATTA TCCTGACAAA 
AAAAGCCTGC CACATGTGCA TGTTGCCATG TGGATAAACT CTCAAGGAGG CCGAAAGGTG 
AAGGCAGGAG ATACAGTGTC ATATATCATT TGTCAGGATG GATCAAATCT CAGTGCCAGC 
CAGCGAGCAT ATGCTCCAGA ACAGTTGCAA AAGCAAGACA ATCTTACTAT TGATACTCAA 
TACTACTTGT CACAGCAAAT ACATCCAGTA GTTGCTAGAA TATGTGAACC TATAGAGGGA 
ATTGATTCTG TTCTTATCGC AACGTGGTTA GGACTGGATC CCTCCCAATT CAGAGTCCAT 
CACCATTACC ATAAAGATGA GGAGAATGAT GCCTTGGTTG GTGGCCCAGC TCAGCTCACT 
GATGAGGAGA AATATAGGGA CTGTGAAAGA TTCAAGTTTT GCTGCCTCAA GTGTGGAACA 
GAGAATATTT ATGACAATGT CTTTGACTGT TCGGGGCGAT TTATTGAGCC CAGCTTGCAA 
AGGTGCAGTA AAAGTGAATG TGAAGAGCCT CCTTTCAACC ATGTGGTTCA AATGAACAAC 
AAATTACTTT TGGATATTAG ACGCTACCTA AAAAAATACT ATAATGGTTG GTTAATATGT 
GAGGATCCAA CTTGCCAGAA TCGAACCCGA CGTCTTCCCC TCAGTTTTTC CCGGAGTGGT 
CCTATATGCC AGGCCTGTAG GAAGGCTATC CTGAGACCAG AGTATTCTGA TAAGGCCCTG 
TACACTCAGC TGTGCTTTTA CCGGTACATT TTTGATGTGG AGTATGCAAC GGATAAAGTG 
ATTACTGAAG AGGATAGAGA GTTCGTAAGG AGGAAGCCCC TCAGGAAGGA AGTGTCCGAG 
GCCTACAGGA AACTGAAGAG CACGGTAGAC AAATGCCTGT CCCTGAGCAG CTACTCGGAA 
GTGAACCTCG GCAAACTCTT CTCCGTCAGC ATGGGAAGAT CCGTGGGAGA GCCCAGCAGT 
GAAAGACAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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POLA1 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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