Pola1 cDNA ORF clone, Mus musculus(house mouse)

The following Pola1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pola1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu56208 XM_006527905.4
Latest version!
Mus musculus polymerase (DNA directed), alpha 1 (Pola1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu15413 NM_008892.2
Latest version!
Mus musculus polymerase (DNA directed), alpha 1 (Pola1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $860.30
$1229.00
View Old Accession Versions >>
OMu56208 XM_006527905.3 Mus musculus polymerase (DNA directed), alpha 1 (Pola1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu56208
    Clone ID Related Accession (Same CDS sequence) XM_006527905.4 , XM_006527905.3
    Accession Version XM_006527905.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4167bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product DNA polymerase alpha catalytic subunit isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006527905.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGCCCA TGCACGAAGA GGACTGTAAA CTGGAGGCAA GCGCTGTGTC AGATTCAGGG 
    AGTTTTGCAG CTTCCCGAGC TCGGCGAGAA AAGAAATCAA AGAAAGGACG TCAAGAAGCT
    TTAGAGAGAC TGAAAAAGGC TAAAGCTGGG GAGAAGTATA AATACGAAGT TGAGGACCTC
    ACAAGTGTTT ATGAAGAAGT TGATGAAGAG CAGTATTCGA AACTGGTTCA GGCCCGTCAG
    GATGATGACT GGATTGTGGA TGACGATGGG ATAGGCTATG TGGAAGATGG CAGAGAGATT
    TTTGATGATG ACCTTGAGGA TGATGCACTT GACACCTGTG GAAAAGGAAG TGATGGCAAA
    GCACACAGAA AGGACAGGAA GGATGTAAAG AAACCTTCAG TGACAAAACC AAACAATATC
    AAGGCAATGT TCATTGCTAG TGCCGGGAAG AAAACTACAG ATAAAGCAGT AGACTTGTCC
    AAGGATGATC TGCTAGGTGA CATTCTACAG GATCTAAACA CTGAGACAGC TCAAATAACT
    CCACCACCTG TATTGATACC AAAGAAAAAA AGATCTACTG GGGCTTTGCT CAACCCTTTT
    TCAGTGCACA CCCCTAAGGC AATTCCTTCA GGAAAACCTG CTTCCCCTGT CCTCAGGAAT
    GAGCCTCTGT TAACTCCCAT TCCTCTTAAA CGTGCTGAAC TTGCTGGAGA GCTGGCTCAG
    CCAGAATGTC CAGAAGATGA GCAGGAGCTA GGGGTAATGG AGTTTGAAGA CGGTGACTTT
    GATGAGTCCA TGGACACAGA GAAGGTAGAT GAGAAGCCTG TGACTGCTAA GACTTGGGAC
    CAAGAGACTG AGCCAGTAGA AAGGGTGGAA CATGAGGCAG ATCCTGAGAG GGGAACCACA
    TCTTACTTAG AAAATTTTCT GCCAGATGTC TCTTGTTGGG ACATTGATCA GGATGATGAA
    AGCATACCTC AGGAAGTTCA AGTGGATTCC AGTAATCTCC CATTGGTAAA AGGGGCAGAT
    GATGAACAAG TGTTTCAGTT TTATTGGCTG GATGCTTATG AAGATCCATA CAACCAACCA
    GGTGTGGTTT TTCTGTTTGG CAAAGTGTGG ATTGAATCAG TCAAAACCCA TGTGAGCTGT
    TGTGTTATGG TGAAAAATAT TGAGCGAACA CTCTACTTCC TTCCCCGTGA AATGAAATTT
    GACCTAAACA CAGGGAAAGA AACAGCAATT CCAGTTACAA TGAAGGATGT TTATGAAGAA
    TTTGATTCAA AAATTTCAGC AAAATATAAA ATAATGAAGT TCAAGTCCAA GATAGTGGAA
    AAGAACTATG CTTTTGAGAT ACCTGATGTT CCAGAAAAAT CTGAGTACCT GGAAGTTAGA
    TACTCGGCAG AAGTGCCACA GCTTCCACAG AATTTAAAAG GAGAAACATT TTCTCATGTG
    TTTGGAACTA ACACGTCTAG CTTGGAACTG TTCTTGATGA ATAGGAAGAT CAAAGGACCT
    TGTTGGCTTG AAGTAAAAAA TCCACAACTC TTGAATCAGC CAATCAGTTG GTGTAAATTT
    GAGGTAATGG CTTTGAAACC AGACCTGGTG AATGTAATTA AGGATGTCAG TCCTCCTCCA
    CTTGTGGTGA TGTCTTTCAG CATGAAGACA ATGCAGAATG TACAGAACCA TCAACATGAG
    ATTATTGCTA TGGCGGCTTT GGTTCATCAC AGTTTTGCAT TAGATAAAGC ACCTCCAGAG
    CCTCCCTTTC AGACACATTT CTGTGTTGTG TCTAAACCAA AGGACTGTAT TTTCCCATGT
    GATTTCAAAG AAGTGATTTC GAAAAAGAAT ATGAAGGTTG AGATCGCTGC AACAGAAAGA
    ACCTTGATAG GATTTTTCCT TGCAAAAGTT CACAAAATTG ACCCTGACAT TCTTGTGGGT
    CATAATATTT GTAGTTTTGA ACTGGAAGTG CTGCTGCAAA GAATTAATGA GTGCAAGGTT
    CCTTACTGGT CCAAGATAGG TCGGCTGAGG AGATCCAACA TGCCAAAACT TGGGAGCCGG
    AGTGGATTTG GTGAAAGAAA TGCTACCTGT GGCCGAATGA TATGTGATGT GGAAATTTCA
    GCAAAAGAAT TGATTCATTG TAAAAGCTAC CATTTGTCTG AACTTGTTCA GCAGATTCTG
    AAAACTGAGA GGATTGTAAT TCCAACAGAA AATATACGAA ACATGTATAG TGAATCTTCT
    TATCTGTTGT ACCTGTTGGA ACATATCTGG AAAGATGCCA GATTCATTTT GCAGATCATG
    TGTGAGCTAA ATGTTCTTCC ATTAGCATTG CAGATCACTA ACATCGCTGG GAACATTATG
    TCTAGGACAC TGATGGGTGG ACGATCTGAG CGAAATGAGT TTTTGTTGCT TCATGCATTT
    TATGAAAACA ACTATATTGT GCCTGACAAG CAGATTTTCA GAAAACCTCA GCAAAAACTG
    GGAGATGAAG ATGAGGAAAT TGATGGGGAC ACCAATAAAT ACAAGAAAGG GCGTAAGAAA
    GCAACTTACG CTGGAGGCTT GGTTTTGGAC CCCAAAGTTG GTTTTTATGA CAAGTTCATT
    TTGCTCCTGG ATTTCAACAG TTTATATCCT TCCATCATTC AGGAATTTAA CATTTGTTTT
    ACAACAGTGC AAAGAGTTAC TTCAGAGGTG CAGAAAGCTA CAGAGGATGA GGAACAAGAA
    CAGATCCCTG AGTTGCCAGA TCCAAACTTA GAGATGGGCA TTTTGCCCAG AGAGATTCGG
    AAACTGGTAG AGCGGAGAAA ACAAGTCAAA CAGCTAATGA AACAGCAAGA TTTAAATCCA
    GACCTTGTTC TTCAGTATGA CATCCGACAG AAGGCTTTGA AGCTCACAGC AAACAGTATG
    TATGGTTGCC TGGGATTTTC GTACAGCAGA TTTTATGCTA AACCACTGGC TGCCTTGGTG
    ACATACAAAG GAAGGGAGAT TTTGATGCAT ACGAAAGACA TGGTACAGAA GATGAATCTT
    GAAGTTATTT ATGGAGACAC AGATTCAATT ATGATAAATA CCAATAGCAC CAACCTGGAA
    GAAGTATTTA AGTTGGGAAA CAAGGTAAAA AGTGAAGTGA ATAAATTGTA CAAACTGCTT
    GAAATAGACA TTGATGCTGT TTTCAAGTCT CTGCTGTTGC TGAAGAAAAA GAAATATGCT
    GCTCTAGTTG TTGAACCAAC ATCAGATGGG AATTACATTA CCAAGCAGGA GCTGAAAGGA
    TTAGATATAG TTAGAAGAGA CTGGTGTGAT CTTGCCAAGG ACACTGGAAA CTTTGTCATT
    GGCCAGATTC TTTCTGATCA AAGCCGGGAC ACCATTGTGG AAAACATTCA GAAGAGGTTA
    ATAGAAATTG GAGAAAATGT GCTAAATGGC AGTGTCCCAG TGAGCCAGTT TGAAATTAAC
    AAGGCATTGA CAAAGGATCC CCAGGATTAC CCTGATAGAA AAAGCTTACC TCATGTACAT
    GTTGCACTGT GGATAAATTC TCAAGGAGGC AGGAAGGTGA AAGCTGGTGA TACGGTGTCC
    TATGTTATTT GTCAGGATGG GTCAAACCTC ACTGCAACTC AGAGGGCCTA TGCCCCAGAA
    CAATTGCAGA AACTTGACAA TTTAGCCATC GACACCCAAT ACTACTTGGC CCAGCAGATC
    CACCCTGTTG TGGCTCGGAT CTGTGAGCCA ATAGATGGAA TTGATGCTGT ACTCATTGCA
    TTATGGCTAG GACTTGACTC CACTCAATTT AGAGTTCATC AGTATCACAA AGATGAAGAG
    AATGATGCCC TCCTTGGTGG TCCAGCACAG CTAACTGATG AAGAGAAATA CAAGGATTGT
    GAAAAATTCA AATGCCTTTG TCCTTCATGT GGAACTGAAA ATATTTATGA TAATGTCTTT
    GAAGGCTCGG GACTGGATAT GGAACCCAGT TTGTATCGTT GCAGTAATGT TGATTGTAAG
    GTTTCACCGT TGACCTTCAT GGTACAGCTG AGCAACAAAT TGATCATGGA CATTAGACGG
    TGCATTAAAA AGTACTATGA TGGCTGGTTG ATATGTGAAG AGCCAACTTG TTGCAGTCGC
    CTTCGACGTC TTCCCCTTCA TTTCTCCCGA AATGGGCCTC TGTGTCCAGT CTGCATGAAA
    GCTGTGCTTA GACCAGAGAT AAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006527968.1
    CDS277..4443
    Translation

    Target ORF information:

    RefSeq Version XM_006527905.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus polymerase (DNA directed), alpha 1 (Pola1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006527905.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGCCCA TGCACGAAGA GGACTGTAAA CTGGAGGCAA GCGCTGTGTC AGATTCAGGG 
    AGTTTTGCAG CTTCCCGAGC TCGGCGAGAA AAGAAATCAA AGAAAGGACG TCAAGAAGCT
    TTAGAGAGAC TGAAAAAGGC TAAAGCTGGG GAGAAGTATA AATACGAAGT TGAGGACCTC
    ACAAGTGTTT ATGAAGAAGT TGATGAAGAG CAGTATTCGA AACTGGTTCA GGCCCGTCAG
    GATGATGACT GGATTGTGGA TGACGATGGG ATAGGCTATG TGGAAGATGG CAGAGAGATT
    TTTGATGATG ACCTTGAGGA TGATGCACTT GACACCTGTG GAAAAGGAAG TGATGGCAAA
    GCACACAGAA AGGACAGGAA GGATGTAAAG AAACCTTCAG TGACAAAACC AAACAATATC
    AAGGCAATGT TCATTGCTAG TGCCGGGAAG AAAACTACAG ATAAAGCAGT AGACTTGTCC
    AAGGATGATC TGCTAGGTGA CATTCTACAG GATCTAAACA CTGAGACAGC TCAAATAACT
    CCACCACCTG TATTGATACC AAAGAAAAAA AGATCTACTG GGGCTTTGCT CAACCCTTTT
    TCAGTGCACA CCCCTAAGGC AATTCCTTCA GGAAAACCTG CTTCCCCTGT CCTCAGGAAT
    GAGCCTCTGT TAACTCCCAT TCCTCTTAAA CGTGCTGAAC TTGCTGGAGA GCTGGCTCAG
    CCAGAATGTC CAGAAGATGA GCAGGAGCTA GGGGTAATGG AGTTTGAAGA CGGTGACTTT
    GATGAGTCCA TGGACACAGA GAAGGTAGAT GAGAAGCCTG TGACTGCTAA GACTTGGGAC
    CAAGAGACTG AGCCAGTAGA AAGGGTGGAA CATGAGGCAG ATCCTGAGAG GGGAACCACA
    TCTTACTTAG AAAATTTTCT GCCAGATGTC TCTTGTTGGG ACATTGATCA GGATGATGAA
    AGCATACCTC AGGAAGTTCA AGTGGATTCC AGTAATCTCC CATTGGTAAA AGGGGCAGAT
    GATGAACAAG TGTTTCAGTT TTATTGGCTG GATGCTTATG AAGATCCATA CAACCAACCA
    GGTGTGGTTT TTCTGTTTGG CAAAGTGTGG ATTGAATCAG TCAAAACCCA TGTGAGCTGT
    TGTGTTATGG TGAAAAATAT TGAGCGAACA CTCTACTTCC TTCCCCGTGA AATGAAATTT
    GACCTAAACA CAGGGAAAGA AACAGCAATT CCAGTTACAA TGAAGGATGT TTATGAAGAA
    TTTGATTCAA AAATTTCAGC AAAATATAAA ATAATGAAGT TCAAGTCCAA GATAGTGGAA
    AAGAACTATG CTTTTGAGAT ACCTGATGTT CCAGAAAAAT CTGAGTACCT GGAAGTTAGA
    TACTCGGCAG AAGTGCCACA GCTTCCACAG AATTTAAAAG GAGAAACATT TTCTCATGTG
    TTTGGAACTA ACACGTCTAG CTTGGAACTG TTCTTGATGA ATAGGAAGAT CAAAGGACCT
    TGTTGGCTTG AAGTAAAAAA TCCACAACTC TTGAATCAGC CAATCAGTTG GTGTAAATTT
    GAGGTAATGG CTTTGAAACC AGACCTGGTG AATGTAATTA AGGATGTCAG TCCTCCTCCA
    CTTGTGGTGA TGTCTTTCAG CATGAAGACA ATGCAGAATG TACAGAACCA TCAACATGAG
    ATTATTGCTA TGGCGGCTTT GGTTCATCAC AGTTTTGCAT TAGATAAAGC ACCTCCAGAG
    CCTCCCTTTC AGACACATTT CTGTGTTGTG TCTAAACCAA AGGACTGTAT TTTCCCATGT
    GATTTCAAAG AAGTGATTTC GAAAAAGAAT ATGAAGGTTG AGATCGCTGC AACAGAAAGA
    ACCTTGATAG GATTTTTCCT TGCAAAAGTT CACAAAATTG ACCCTGACAT TCTTGTGGGT
    CATAATATTT GTAGTTTTGA ACTGGAAGTG CTGCTGCAAA GAATTAATGA GTGCAAGGTT
    CCTTACTGGT CCAAGATAGG TCGGCTGAGG AGATCCAACA TGCCAAAACT TGGGAGCCGG
    AGTGGATTTG GTGAAAGAAA TGCTACCTGT GGCCGAATGA TATGTGATGT GGAAATTTCA
    GCAAAAGAAT TGATTCATTG TAAAAGCTAC CATTTGTCTG AACTTGTTCA GCAGATTCTG
    AAAACTGAGA GGATTGTAAT TCCAACAGAA AATATACGAA ACATGTATAG TGAATCTTCT
    TATCTGTTGT ACCTGTTGGA ACATATCTGG AAAGATGCCA GATTCATTTT GCAGATCATG
    TGTGAGCTAA ATGTTCTTCC ATTAGCATTG CAGATCACTA ACATCGCTGG GAACATTATG
    TCTAGGACAC TGATGGGTGG ACGATCTGAG CGAAATGAGT TTTTGTTGCT TCATGCATTT
    TATGAAAACA ACTATATTGT GCCTGACAAG CAGATTTTCA GAAAACCTCA GCAAAAACTG
    GGAGATGAAG ATGAGGAAAT TGATGGGGAC ACCAATAAAT ACAAGAAAGG GCGTAAGAAA
    GCAACTTACG CTGGAGGCTT GGTTTTGGAC CCCAAAGTTG GTTTTTATGA CAAGTTCATT
    TTGCTCCTGG ATTTCAACAG TTTATATCCT TCCATCATTC AGGAATTTAA CATTTGTTTT
    ACAACAGTGC AAAGAGTTAC TTCAGAGGTG CAGAAAGCTA CAGAGGATGA GGAACAAGAA
    CAGATCCCTG AGTTGCCAGA TCCAAACTTA GAGATGGGCA TTTTGCCCAG AGAGATTCGG
    AAACTGGTAG AGCGGAGAAA ACAAGTCAAA CAGCTAATGA AACAGCAAGA TTTAAATCCA
    GACCTTGTTC TTCAGTATGA CATCCGACAG AAGGCTTTGA AGCTCACAGC AAACAGTATG
    TATGGTTGCC TGGGATTTTC GTACAGCAGA TTTTATGCTA AACCACTGGC TGCCTTGGTG
    ACATACAAAG GAAGGGAGAT TTTGATGCAT ACGAAAGACA TGGTACAGAA GATGAATCTT
    GAAGTTATTT ATGGAGACAC AGATTCAATT ATGATAAATA CCAATAGCAC CAACCTGGAA
    GAAGTATTTA AGTTGGGAAA CAAGGTAAAA AGTGAAGTGA ATAAATTGTA CAAACTGCTT
    GAAATAGACA TTGATGCTGT TTTCAAGTCT CTGCTGTTGC TGAAGAAAAA GAAATATGCT
    GCTCTAGTTG TTGAACCAAC ATCAGATGGG AATTACATTA CCAAGCAGGA GCTGAAAGGA
    TTAGATATAG TTAGAAGAGA CTGGTGTGAT CTTGCCAAGG ACACTGGAAA CTTTGTCATT
    GGCCAGATTC TTTCTGATCA AAGCCGGGAC ACCATTGTGG AAAACATTCA GAAGAGGTTA
    ATAGAAATTG GAGAAAATGT GCTAAATGGC AGTGTCCCAG TGAGCCAGTT TGAAATTAAC
    AAGGCATTGA CAAAGGATCC CCAGGATTAC CCTGATAGAA AAAGCTTACC TCATGTACAT
    GTTGCACTGT GGATAAATTC TCAAGGAGGC AGGAAGGTGA AAGCTGGTGA TACGGTGTCC
    TATGTTATTT GTCAGGATGG GTCAAACCTC ACTGCAACTC AGAGGGCCTA TGCCCCAGAA
    CAATTGCAGA AACTTGACAA TTTAGCCATC GACACCCAAT ACTACTTGGC CCAGCAGATC
    CACCCTGTTG TGGCTCGGAT CTGTGAGCCA ATAGATGGAA TTGATGCTGT ACTCATTGCA
    TTATGGCTAG GACTTGACTC CACTCAATTT AGAGTTCATC AGTATCACAA AGATGAAGAG
    AATGATGCCC TCCTTGGTGG TCCAGCACAG CTAACTGATG AAGAGAAATA CAAGGATTGT
    GAAAAATTCA AATGCCTTTG TCCTTCATGT GGAACTGAAA ATATTTATGA TAATGTCTTT
    GAAGGCTCGG GACTGGATAT GGAACCCAGT TTGTATCGTT GCAGTAATGT TGATTGTAAG
    GTTTCACCGT TGACCTTCAT GGTACAGCTG AGCAACAAAT TGATCATGGA CATTAGACGG
    TGCATTAAAA AGTACTATGA TGGCTGGTTG ATATGTGAAG AGCCAACTTG TTGCAGTCGC
    CTTCGACGTC TTCCCCTTCA TTTCTCCCGA AATGGGCCTC TGTGTCCAGT CTGCATGAAA
    GCTGTGCTTA GACCAGAGAT AAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu15413
    Clone ID Related Accession (Same CDS sequence) NM_008892.2
    Accession Version NM_008892.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4398bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-21
    Organism Mus musculus(house mouse)
    Product DNA polymerase alpha catalytic subunit
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from D17384.1, BC138047.1 and CJ133126.1. On Oct 31, 2009 this sequence version replaced NM_008892.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D17384.1, D13543.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGCGCCCA TGCACGAAGA GGACTGTAAA CTGGAGGCAA GCGCTGTGTC AGATTCAGGG 
    AGTTTTGCAG CTTCCCGAGC TCGGCGAGAA AAGAAATCAA AGAAAGGACG TCAAGAAGCT
    TTAGAGAGAC TGAAAAAGGC TAAAGCTGGG GAGAAGTATA AATACGAAGT TGAGGACCTC
    ACAAGTGTTT ATGAAGAAGT TGATGAAGAG CAGTATTCGA AACTGGTTCA GGCCCGTCAG
    GATGATGACT GGATTGTGGA TGACGATGGG ATAGGCTATG TGGAAGATGG CAGAGAGATT
    TTTGATGATG ACCTTGAGGA TGATGCACTT GACACCTGTG GAAAAGGAAG TGATGGCAAA
    GCACACAGAA AGGACAGGAA GGATGTAAAG AAACCTTCAG TGACAAAACC AAACAATATC
    AAGGCAATGT TCATTGCTAG TGCCGGGAAG AAAACTACAG ATAAAGCAGT AGACTTGTCC
    AAGGATGATC TGCTAGGTGA CATTCTACAG GATCTAAACA CTGAGACAGC TCAAATAACT
    CCACCACCTG TATTGATACC AAAGAAAAAA AGATCTACTG GGGCTTTGCT CAACCCTTTT
    TCAGTGCACA CCCCTAAGGC AATTCCTTCA GGAAAACCTG CTTCCCCTGT CCTCAGGAAT
    GAGCCTCTGT TAACTCCCAT TCCTCTTAAA CGTGCTGAAC TTGCTGGAGA GCTGGCTCAG
    CCAGAATGTC CAGAAGATGA GCAGGAGCTA GGGGTAATGG AGTTTGAAGA CGGTGACTTT
    GATGAGTCCA TGGACACAGA GAAGGTAGAT GAGAAGCCTG TGACTGCTAA GACTTGGGAC
    CAAGAGACTG AGCCAGTAGA AAGGGTGGAA CATGAGGCAG ATCCTGAGAG GGGAACCACA
    TCTTACTTAG AAAATTTTCT GCCAGATGTC TCTTGTTGGG ACATTGATCA GGATGATGAA
    AGCATACCTC AGGAAGTTCA AGTGGATTCC AGTAATCTCC CATTGGTAAA AGGGGCAGAT
    GATGAACAAG TGTTTCAGTT TTATTGGCTG GATGCTTATG AAGATCCATA CAACCAACCA
    GGTGTGGTTT TTCTGTTTGG CAAAGTGTGG ATTGAATCAG TCAAAACCCA TGTGAGCTGT
    TGTGTTATGG TGAAAAATAT TGAGCGAACA CTCTACTTCC TTCCCCGTGA AATGAAATTT
    GACCTAAACA CAGGGAAAGA AACAGCAATT CCAGTTACAA TGAAGGATGT TTATGAAGAA
    TTTGATTCAA AAATTTCAGC AAAATATAAA ATAATGAAGT TCAAGTCCAA GATAGTGGAA
    AAGAACTATG CTTTTGAGAT ACCTGATGTT CCAGAAAAAT CTGAGTACCT GGAAGTTAGA
    TACTCGGCAG AAGTGCCACA GCTTCCACAG AATTTAAAAG GAGAAACATT TTCTCATGTG
    TTTGGAACTA ACACGTCTAG CTTGGAACTG TTCTTGATGA ATAGGAAGAT CAAAGGACCT
    TGTTGGCTTG AAGTAAAAAA TCCACAACTC TTGAATCAGC CAATCAGTTG GTGTAAATTT
    GAGGTAATGG CTTTGAAACC AGACCTGGTG AATGTAATTA AGGATGTCAG TCCTCCTCCA
    CTTGTGGTGA TGTCTTTCAG CATGAAGACA ATGCAGAATG TACAGAACCA TCAACATGAG
    ATTATTGCTA TGGCGGCTTT GGTTCATCAC AGTTTTGCAT TAGATAAAGC ACCTCCAGAG
    CCTCCCTTTC AGACACATTT CTGTGTTGTG TCTAAACCAA AGGACTGTAT TTTCCCATGT
    GATTTCAAAG AAGTGATTTC GAAAAAGAAT ATGAAGGTTG AGATCGCTGC AACAGAAAGA
    ACCTTGATAG GATTTTTCCT TGCAAAAGTT CACAAAATTG ACCCTGACAT TCTTGTGGGT
    CATAATATTT GTAGTTTTGA ACTGGAAGTG CTGCTGCAAA GAATTAATGA GTGCAAGGTT
    CCTTACTGGT CCAAGATAGG TCGGCTGAGG AGATCCAACA TGCCAAAACT TGGGAGCCGG
    AGTGGATTTG GTGAAAGAAA TGCTACCTGT GGCCGAATGA TATGTGATGT GGAAATTTCA
    GCAAAAGAAT TGATTCATTG TAAAAGCTAC CATTTGTCTG AACTTGTTCA GCAGATTCTG
    AAAACTGAGA GGATTGTAAT TCCAACAGAA AATATACGAA ACATGTATAG TGAATCTTCT
    TATCTGTTGT ACCTGTTGGA ACATATCTGG AAAGATGCCA GATTCATTTT GCAGATCATG
    TGTGAGCTAA ATGTTCTTCC ATTAGCATTG CAGATCACTA ACATCGCTGG GAACATTATG
    TCTAGGACAC TGATGGGTGG ACGATCTGAG CGAAATGAGT TTTTGTTGCT TCATGCATTT
    TATGAAAACA ACTATATTGT GCCTGACAAG CAGATTTTCA GAAAACCTCA GCAAAAACTG
    GGAGATGAAG ATGAGGAAAT TGATGGGGAC ACCAATAAAT ACAAGAAAGG GCGTAAGAAA
    GCAACTTACG CTGGAGGCTT GGTTTTGGAC CCCAAAGTTG GTTTTTATGA CAAGTTCATT
    TTGCTCCTGG ATTTCAACAG TTTATATCCT TCCATCATTC AGGAATTTAA CATTTGTTTT
    ACAACAGTGC AAAGAGTTAC TTCAGAGGTG CAGAAAGCTA CAGAGGATGA GGAACAAGAA
    CAGATCCCTG AGTTGCCAGA TCCAAACTTA GAGATGGGCA TTTTGCCCAG AGAGATTCGG
    AAACTGGTAG AGCGGAGAAA ACAAGTCAAA CAGCTAATGA AACAGCAAGA TTTAAATCCA
    GACCTTGTTC TTCAGTATGA CATCCGACAG AAGGCTTTGA AGCTCACAGC AAACAGTATG
    TATGGTTGCC TGGGATTTTC GTACAGCAGA TTTTATGCTA AACCACTGGC TGCCTTGGTG
    ACATACAAAG GAAGGGAGAT TTTGATGCAT ACGAAAGACA TGGTACAGAA GATGAATCTT
    GAAGTTATTT ATGGAGACAC AGATTCAATT ATGATAAATA CCAATAGCAC CAACCTGGAA
    GAAGTATTTA AGTTGGGAAA CAAGGTAAAA AGTGAAGTGA ATAAATTGTA CAAACTGCTT
    GAAATAGACA TTGATGCTGT TTTCAAGTCT CTGCTGTTGC TGAAGAAAAA GAAATATGCT
    GCTCTAGTTG TTGAACCAAC ATCAGATGGG AATTACATTA CCAAGCAGGA GCTGAAAGGA
    TTAGATATAG TTAGAAGAGA CTGGTGTGAT CTTGCCAAGG ACACTGGAAA CTTTGTCATT
    GGCCAGATTC TTTCTGATCA AAGCCGGGAC ACCATTGTGG AAAACATTCA GAAGAGGTTA
    ATAGAAATTG GAGAAAATGT GCTAAATGGC AGTGTCCCAG TGAGCCAGTT TGAAATTAAC
    AAGGCATTGA CAAAGGATCC CCAGGATTAC CCTGATAGAA AAAGCTTACC TCATGTACAT
    GTTGCACTGT GGATAAATTC TCAAGGAGGC AGGAAGGTGA AAGCTGGTGA TACGGTGTCC
    TATGTTATTT GTCAGGATGG GTCAAACCTC ACTGCAACTC AGAGGGCCTA TGCCCCAGAA
    CAATTGCAGA AACTTGACAA TTTAGCCATC GACACCCAAT ACTACTTGGC CCAGCAGATC
    CACCCTGTTG TGGCTCGGAT CTGTGAGCCA ATAGATGGAA TTGATGCTGT ACTCATTGCA
    TTATGGCTAG GACTTGACTC CACTCAATTT AGAGTTCATC AGTATCACAA AGATGAAGAG
    AATGATGCCC TCCTTGGTGG TCCAGCACAG CTAACTGATG AAGAGAAATA CAAGGATTGT
    GAAAAATTCA AATGCCTTTG TCCTTCATGT GGAACTGAAA ATATTTATGA TAATGTCTTT
    GAAGGCTCGG GACTGGATAT GGAACCCAGT TTGTATCGTT GCAGTAATGT TGATTGTAAG
    GTTTCACCGT TGACCTTCAT GGTACAGCTG AGCAACAAAT TGATCATGGA CATTAGACGG
    TGCATTAAAA AGTACTATGA TGGCTGGTTG ATATGTGAAG AGCCAACTTG TTGCAGTCGC
    CTTCGACGTC TTCCCCTTCA TTTCTCCCGA AATGGGCCTC TGTGTCCAGT CTGCATGAAA
    GCTGTGCTTA GACCAGAGTA TTCTGACAAG TCCTTGTACA CCCAGCTGTG CTTTTACCGG
    TACATTTTTG ATGCGGACTG TGCACTGGAA AAACTTACTG AACATGAGAA AGATAAATTG
    AAGAAGCAGT TTTTCCCTCT CCGAGTTCTT CAGGACTACA GAAAAGTCAA GAACATAGCA
    GAGCAGTTCT TGTCCTGGAG TGGCTACTCT GAAGTGAACC TCAGCAAGCT CTTCGCCAAT
    TACGCTGGGA AGTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_032918.1
    CDS45..4442
    Translation

    Target ORF information:

    RefSeq Version NM_008892.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus polymerase (DNA directed), alpha 1 (Pola1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_008892.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGCGCCCA TGCACGAAGA GGACTGTAAA CTGGAGGCAA GCGCTGTGTC AGATTCAGGG 
    AGTTTTGCAG CTTCCCGAGC TCGGCGAGAA AAGAAATCAA AGAAAGGACG TCAAGAAGCT
    TTAGAGAGAC TGAAAAAGGC TAAAGCTGGG GAGAAGTATA AATACGAAGT TGAGGACCTC
    ACAAGTGTTT ATGAAGAAGT TGATGAAGAG CAGTATTCGA AACTGGTTCA GGCCCGTCAG
    GATGATGACT GGATTGTGGA TGACGATGGG ATAGGCTATG TGGAAGATGG CAGAGAGATT
    TTTGATGATG ACCTTGAGGA TGATGCACTT GACACCTGTG GAAAAGGAAG TGATGGCAAA
    GCACACAGAA AGGACAGGAA GGATGTAAAG AAACCTTCAG TGACAAAACC AAACAATATC
    AAGGCAATGT TCATTGCTAG TGCCGGGAAG AAAACTACAG ATAAAGCAGT AGACTTGTCC
    AAGGATGATC TGCTAGGTGA CATTCTACAG GATCTAAACA CTGAGACAGC TCAAATAACT
    CCACCACCTG TATTGATACC AAAGAAAAAA AGATCTACTG GGGCTTTGCT CAACCCTTTT
    TCAGTGCACA CCCCTAAGGC AATTCCTTCA GGAAAACCTG CTTCCCCTGT CCTCAGGAAT
    GAGCCTCTGT TAACTCCCAT TCCTCTTAAA CGTGCTGAAC TTGCTGGAGA GCTGGCTCAG
    CCAGAATGTC CAGAAGATGA GCAGGAGCTA GGGGTAATGG AGTTTGAAGA CGGTGACTTT
    GATGAGTCCA TGGACACAGA GAAGGTAGAT GAGAAGCCTG TGACTGCTAA GACTTGGGAC
    CAAGAGACTG AGCCAGTAGA AAGGGTGGAA CATGAGGCAG ATCCTGAGAG GGGAACCACA
    TCTTACTTAG AAAATTTTCT GCCAGATGTC TCTTGTTGGG ACATTGATCA GGATGATGAA
    AGCATACCTC AGGAAGTTCA AGTGGATTCC AGTAATCTCC CATTGGTAAA AGGGGCAGAT
    GATGAACAAG TGTTTCAGTT TTATTGGCTG GATGCTTATG AAGATCCATA CAACCAACCA
    GGTGTGGTTT TTCTGTTTGG CAAAGTGTGG ATTGAATCAG TCAAAACCCA TGTGAGCTGT
    TGTGTTATGG TGAAAAATAT TGAGCGAACA CTCTACTTCC TTCCCCGTGA AATGAAATTT
    GACCTAAACA CAGGGAAAGA AACAGCAATT CCAGTTACAA TGAAGGATGT TTATGAAGAA
    TTTGATTCAA AAATTTCAGC AAAATATAAA ATAATGAAGT TCAAGTCCAA GATAGTGGAA
    AAGAACTATG CTTTTGAGAT ACCTGATGTT CCAGAAAAAT CTGAGTACCT GGAAGTTAGA
    TACTCGGCAG AAGTGCCACA GCTTCCACAG AATTTAAAAG GAGAAACATT TTCTCATGTG
    TTTGGAACTA ACACGTCTAG CTTGGAACTG TTCTTGATGA ATAGGAAGAT CAAAGGACCT
    TGTTGGCTTG AAGTAAAAAA TCCACAACTC TTGAATCAGC CAATCAGTTG GTGTAAATTT
    GAGGTAATGG CTTTGAAACC AGACCTGGTG AATGTAATTA AGGATGTCAG TCCTCCTCCA
    CTTGTGGTGA TGTCTTTCAG CATGAAGACA ATGCAGAATG TACAGAACCA TCAACATGAG
    ATTATTGCTA TGGCGGCTTT GGTTCATCAC AGTTTTGCAT TAGATAAAGC ACCTCCAGAG
    CCTCCCTTTC AGACACATTT CTGTGTTGTG TCTAAACCAA AGGACTGTAT TTTCCCATGT
    GATTTCAAAG AAGTGATTTC GAAAAAGAAT ATGAAGGTTG AGATCGCTGC AACAGAAAGA
    ACCTTGATAG GATTTTTCCT TGCAAAAGTT CACAAAATTG ACCCTGACAT TCTTGTGGGT
    CATAATATTT GTAGTTTTGA ACTGGAAGTG CTGCTGCAAA GAATTAATGA GTGCAAGGTT
    CCTTACTGGT CCAAGATAGG TCGGCTGAGG AGATCCAACA TGCCAAAACT TGGGAGCCGG
    AGTGGATTTG GTGAAAGAAA TGCTACCTGT GGCCGAATGA TATGTGATGT GGAAATTTCA
    GCAAAAGAAT TGATTCATTG TAAAAGCTAC CATTTGTCTG AACTTGTTCA GCAGATTCTG
    AAAACTGAGA GGATTGTAAT TCCAACAGAA AATATACGAA ACATGTATAG TGAATCTTCT
    TATCTGTTGT ACCTGTTGGA ACATATCTGG AAAGATGCCA GATTCATTTT GCAGATCATG
    TGTGAGCTAA ATGTTCTTCC ATTAGCATTG CAGATCACTA ACATCGCTGG GAACATTATG
    TCTAGGACAC TGATGGGTGG ACGATCTGAG CGAAATGAGT TTTTGTTGCT TCATGCATTT
    TATGAAAACA ACTATATTGT GCCTGACAAG CAGATTTTCA GAAAACCTCA GCAAAAACTG
    GGAGATGAAG ATGAGGAAAT TGATGGGGAC ACCAATAAAT ACAAGAAAGG GCGTAAGAAA
    GCAACTTACG CTGGAGGCTT GGTTTTGGAC CCCAAAGTTG GTTTTTATGA CAAGTTCATT
    TTGCTCCTGG ATTTCAACAG TTTATATCCT TCCATCATTC AGGAATTTAA CATTTGTTTT
    ACAACAGTGC AAAGAGTTAC TTCAGAGGTG CAGAAAGCTA CAGAGGATGA GGAACAAGAA
    CAGATCCCTG AGTTGCCAGA TCCAAACTTA GAGATGGGCA TTTTGCCCAG AGAGATTCGG
    AAACTGGTAG AGCGGAGAAA ACAAGTCAAA CAGCTAATGA AACAGCAAGA TTTAAATCCA
    GACCTTGTTC TTCAGTATGA CATCCGACAG AAGGCTTTGA AGCTCACAGC AAACAGTATG
    TATGGTTGCC TGGGATTTTC GTACAGCAGA TTTTATGCTA AACCACTGGC TGCCTTGGTG
    ACATACAAAG GAAGGGAGAT TTTGATGCAT ACGAAAGACA TGGTACAGAA GATGAATCTT
    GAAGTTATTT ATGGAGACAC AGATTCAATT ATGATAAATA CCAATAGCAC CAACCTGGAA
    GAAGTATTTA AGTTGGGAAA CAAGGTAAAA AGTGAAGTGA ATAAATTGTA CAAACTGCTT
    GAAATAGACA TTGATGCTGT TTTCAAGTCT CTGCTGTTGC TGAAGAAAAA GAAATATGCT
    GCTCTAGTTG TTGAACCAAC ATCAGATGGG AATTACATTA CCAAGCAGGA GCTGAAAGGA
    TTAGATATAG TTAGAAGAGA CTGGTGTGAT CTTGCCAAGG ACACTGGAAA CTTTGTCATT
    GGCCAGATTC TTTCTGATCA AAGCCGGGAC ACCATTGTGG AAAACATTCA GAAGAGGTTA
    ATAGAAATTG GAGAAAATGT GCTAAATGGC AGTGTCCCAG TGAGCCAGTT TGAAATTAAC
    AAGGCATTGA CAAAGGATCC CCAGGATTAC CCTGATAGAA AAAGCTTACC TCATGTACAT
    GTTGCACTGT GGATAAATTC TCAAGGAGGC AGGAAGGTGA AAGCTGGTGA TACGGTGTCC
    TATGTTATTT GTCAGGATGG GTCAAACCTC ACTGCAACTC AGAGGGCCTA TGCCCCAGAA
    CAATTGCAGA AACTTGACAA TTTAGCCATC GACACCCAAT ACTACTTGGC CCAGCAGATC
    CACCCTGTTG TGGCTCGGAT CTGTGAGCCA ATAGATGGAA TTGATGCTGT ACTCATTGCA
    TTATGGCTAG GACTTGACTC CACTCAATTT AGAGTTCATC AGTATCACAA AGATGAAGAG
    AATGATGCCC TCCTTGGTGG TCCAGCACAG CTAACTGATG AAGAGAAATA CAAGGATTGT
    GAAAAATTCA AATGCCTTTG TCCTTCATGT GGAACTGAAA ATATTTATGA TAATGTCTTT
    GAAGGCTCGG GACTGGATAT GGAACCCAGT TTGTATCGTT GCAGTAATGT TGATTGTAAG
    GTTTCACCGT TGACCTTCAT GGTACAGCTG AGCAACAAAT TGATCATGGA CATTAGACGG
    TGCATTAAAA AGTACTATGA TGGCTGGTTG ATATGTGAAG AGCCAACTTG TTGCAGTCGC
    CTTCGACGTC TTCCCCTTCA TTTCTCCCGA AATGGGCCTC TGTGTCCAGT CTGCATGAAA
    GCTGTGCTTA GACCAGAGTA TTCTGACAAG TCCTTGTACA CCCAGCTGTG CTTTTACCGG
    TACATTTTTG ATGCGGACTG TGCACTGGAA AAACTTACTG AACATGAGAA AGATAAATTG
    AAGAAGCAGT TTTTCCCTCT CCGAGTTCTT CAGGACTACA GAAAAGTCAA GAACATAGCA
    GAGCAGTTCT TGTCCTGGAG TGGCTACTCT GAAGTGAACC TCAGCAAGCT CTTCGCCAAT
    TACGCTGGGA AGTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu56208
    Clone ID Related Accession (Same CDS sequence) XM_006527905.4 , XM_006527905.3
    Accession Version XM_006527905.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4167bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product DNA polymerase alpha catalytic subunit isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006527905.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGCCCA TGCACGAAGA GGACTGTAAA CTGGAGGCAA GCGCTGTGTC AGATTCAGGG 
    AGTTTTGCAG CTTCCCGAGC TCGGCGAGAA AAGAAATCAA AGAAAGGACG TCAAGAAGCT
    TTAGAGAGAC TGAAAAAGGC TAAAGCTGGG GAGAAGTATA AATACGAAGT TGAGGACCTC
    ACAAGTGTTT ATGAAGAAGT TGATGAAGAG CAGTATTCGA AACTGGTTCA GGCCCGTCAG
    GATGATGACT GGATTGTGGA TGACGATGGG ATAGGCTATG TGGAAGATGG CAGAGAGATT
    TTTGATGATG ACCTTGAGGA TGATGCACTT GACACCTGTG GAAAAGGAAG TGATGGCAAA
    GCACACAGAA AGGACAGGAA GGATGTAAAG AAACCTTCAG TGACAAAACC AAACAATATC
    AAGGCAATGT TCATTGCTAG TGCCGGGAAG AAAACTACAG ATAAAGCAGT AGACTTGTCC
    AAGGATGATC TGCTAGGTGA CATTCTACAG GATCTAAACA CTGAGACAGC TCAAATAACT
    CCACCACCTG TATTGATACC AAAGAAAAAA AGATCTACTG GGGCTTTGCT CAACCCTTTT
    TCAGTGCACA CCCCTAAGGC AATTCCTTCA GGAAAACCTG CTTCCCCTGT CCTCAGGAAT
    GAGCCTCTGT TAACTCCCAT TCCTCTTAAA CGTGCTGAAC TTGCTGGAGA GCTGGCTCAG
    CCAGAATGTC CAGAAGATGA GCAGGAGCTA GGGGTAATGG AGTTTGAAGA CGGTGACTTT
    GATGAGTCCA TGGACACAGA GAAGGTAGAT GAGAAGCCTG TGACTGCTAA GACTTGGGAC
    CAAGAGACTG AGCCAGTAGA AAGGGTGGAA CATGAGGCAG ATCCTGAGAG GGGAACCACA
    TCTTACTTAG AAAATTTTCT GCCAGATGTC TCTTGTTGGG ACATTGATCA GGATGATGAA
    AGCATACCTC AGGAAGTTCA AGTGGATTCC AGTAATCTCC CATTGGTAAA AGGGGCAGAT
    GATGAACAAG TGTTTCAGTT TTATTGGCTG GATGCTTATG AAGATCCATA CAACCAACCA
    GGTGTGGTTT TTCTGTTTGG CAAAGTGTGG ATTGAATCAG TCAAAACCCA TGTGAGCTGT
    TGTGTTATGG TGAAAAATAT TGAGCGAACA CTCTACTTCC TTCCCCGTGA AATGAAATTT
    GACCTAAACA CAGGGAAAGA AACAGCAATT CCAGTTACAA TGAAGGATGT TTATGAAGAA
    TTTGATTCAA AAATTTCAGC AAAATATAAA ATAATGAAGT TCAAGTCCAA GATAGTGGAA
    AAGAACTATG CTTTTGAGAT ACCTGATGTT CCAGAAAAAT CTGAGTACCT GGAAGTTAGA
    TACTCGGCAG AAGTGCCACA GCTTCCACAG AATTTAAAAG GAGAAACATT TTCTCATGTG
    TTTGGAACTA ACACGTCTAG CTTGGAACTG TTCTTGATGA ATAGGAAGAT CAAAGGACCT
    TGTTGGCTTG AAGTAAAAAA TCCACAACTC TTGAATCAGC CAATCAGTTG GTGTAAATTT
    GAGGTAATGG CTTTGAAACC AGACCTGGTG AATGTAATTA AGGATGTCAG TCCTCCTCCA
    CTTGTGGTGA TGTCTTTCAG CATGAAGACA ATGCAGAATG TACAGAACCA TCAACATGAG
    ATTATTGCTA TGGCGGCTTT GGTTCATCAC AGTTTTGCAT TAGATAAAGC ACCTCCAGAG
    CCTCCCTTTC AGACACATTT CTGTGTTGTG TCTAAACCAA AGGACTGTAT TTTCCCATGT
    GATTTCAAAG AAGTGATTTC GAAAAAGAAT ATGAAGGTTG AGATCGCTGC AACAGAAAGA
    ACCTTGATAG GATTTTTCCT TGCAAAAGTT CACAAAATTG ACCCTGACAT TCTTGTGGGT
    CATAATATTT GTAGTTTTGA ACTGGAAGTG CTGCTGCAAA GAATTAATGA GTGCAAGGTT
    CCTTACTGGT CCAAGATAGG TCGGCTGAGG AGATCCAACA TGCCAAAACT TGGGAGCCGG
    AGTGGATTTG GTGAAAGAAA TGCTACCTGT GGCCGAATGA TATGTGATGT GGAAATTTCA
    GCAAAAGAAT TGATTCATTG TAAAAGCTAC CATTTGTCTG AACTTGTTCA GCAGATTCTG
    AAAACTGAGA GGATTGTAAT TCCAACAGAA AATATACGAA ACATGTATAG TGAATCTTCT
    TATCTGTTGT ACCTGTTGGA ACATATCTGG AAAGATGCCA GATTCATTTT GCAGATCATG
    TGTGAGCTAA ATGTTCTTCC ATTAGCATTG CAGATCACTA ACATCGCTGG GAACATTATG
    TCTAGGACAC TGATGGGTGG ACGATCTGAG CGAAATGAGT TTTTGTTGCT TCATGCATTT
    TATGAAAACA ACTATATTGT GCCTGACAAG CAGATTTTCA GAAAACCTCA GCAAAAACTG
    GGAGATGAAG ATGAGGAAAT TGATGGGGAC ACCAATAAAT ACAAGAAAGG GCGTAAGAAA
    GCAACTTACG CTGGAGGCTT GGTTTTGGAC CCCAAAGTTG GTTTTTATGA CAAGTTCATT
    TTGCTCCTGG ATTTCAACAG TTTATATCCT TCCATCATTC AGGAATTTAA CATTTGTTTT
    ACAACAGTGC AAAGAGTTAC TTCAGAGGTG CAGAAAGCTA CAGAGGATGA GGAACAAGAA
    CAGATCCCTG AGTTGCCAGA TCCAAACTTA GAGATGGGCA TTTTGCCCAG AGAGATTCGG
    AAACTGGTAG AGCGGAGAAA ACAAGTCAAA CAGCTAATGA AACAGCAAGA TTTAAATCCA
    GACCTTGTTC TTCAGTATGA CATCCGACAG AAGGCTTTGA AGCTCACAGC AAACAGTATG
    TATGGTTGCC TGGGATTTTC GTACAGCAGA TTTTATGCTA AACCACTGGC TGCCTTGGTG
    ACATACAAAG GAAGGGAGAT TTTGATGCAT ACGAAAGACA TGGTACAGAA GATGAATCTT
    GAAGTTATTT ATGGAGACAC AGATTCAATT ATGATAAATA CCAATAGCAC CAACCTGGAA
    GAAGTATTTA AGTTGGGAAA CAAGGTAAAA AGTGAAGTGA ATAAATTGTA CAAACTGCTT
    GAAATAGACA TTGATGCTGT TTTCAAGTCT CTGCTGTTGC TGAAGAAAAA GAAATATGCT
    GCTCTAGTTG TTGAACCAAC ATCAGATGGG AATTACATTA CCAAGCAGGA GCTGAAAGGA
    TTAGATATAG TTAGAAGAGA CTGGTGTGAT CTTGCCAAGG ACACTGGAAA CTTTGTCATT
    GGCCAGATTC TTTCTGATCA AAGCCGGGAC ACCATTGTGG AAAACATTCA GAAGAGGTTA
    ATAGAAATTG GAGAAAATGT GCTAAATGGC AGTGTCCCAG TGAGCCAGTT TGAAATTAAC
    AAGGCATTGA CAAAGGATCC CCAGGATTAC CCTGATAGAA AAAGCTTACC TCATGTACAT
    GTTGCACTGT GGATAAATTC TCAAGGAGGC AGGAAGGTGA AAGCTGGTGA TACGGTGTCC
    TATGTTATTT GTCAGGATGG GTCAAACCTC ACTGCAACTC AGAGGGCCTA TGCCCCAGAA
    CAATTGCAGA AACTTGACAA TTTAGCCATC GACACCCAAT ACTACTTGGC CCAGCAGATC
    CACCCTGTTG TGGCTCGGAT CTGTGAGCCA ATAGATGGAA TTGATGCTGT ACTCATTGCA
    TTATGGCTAG GACTTGACTC CACTCAATTT AGAGTTCATC AGTATCACAA AGATGAAGAG
    AATGATGCCC TCCTTGGTGG TCCAGCACAG CTAACTGATG AAGAGAAATA CAAGGATTGT
    GAAAAATTCA AATGCCTTTG TCCTTCATGT GGAACTGAAA ATATTTATGA TAATGTCTTT
    GAAGGCTCGG GACTGGATAT GGAACCCAGT TTGTATCGTT GCAGTAATGT TGATTGTAAG
    GTTTCACCGT TGACCTTCAT GGTACAGCTG AGCAACAAAT TGATCATGGA CATTAGACGG
    TGCATTAAAA AGTACTATGA TGGCTGGTTG ATATGTGAAG AGCCAACTTG TTGCAGTCGC
    CTTCGACGTC TTCCCCTTCA TTTCTCCCGA AATGGGCCTC TGTGTCCAGT CTGCATGAAA
    GCTGTGCTTA GACCAGAGAT AAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006527968.1
    CDS99..4265
    Translation

    Target ORF information:

    RefSeq Version XM_006527905.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus polymerase (DNA directed), alpha 1 (Pola1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006527905.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGCCCA TGCACGAAGA GGACTGTAAA CTGGAGGCAA GCGCTGTGTC AGATTCAGGG 
    AGTTTTGCAG CTTCCCGAGC TCGGCGAGAA AAGAAATCAA AGAAAGGACG TCAAGAAGCT
    TTAGAGAGAC TGAAAAAGGC TAAAGCTGGG GAGAAGTATA AATACGAAGT TGAGGACCTC
    ACAAGTGTTT ATGAAGAAGT TGATGAAGAG CAGTATTCGA AACTGGTTCA GGCCCGTCAG
    GATGATGACT GGATTGTGGA TGACGATGGG ATAGGCTATG TGGAAGATGG CAGAGAGATT
    TTTGATGATG ACCTTGAGGA TGATGCACTT GACACCTGTG GAAAAGGAAG TGATGGCAAA
    GCACACAGAA AGGACAGGAA GGATGTAAAG AAACCTTCAG TGACAAAACC AAACAATATC
    AAGGCAATGT TCATTGCTAG TGCCGGGAAG AAAACTACAG ATAAAGCAGT AGACTTGTCC
    AAGGATGATC TGCTAGGTGA CATTCTACAG GATCTAAACA CTGAGACAGC TCAAATAACT
    CCACCACCTG TATTGATACC AAAGAAAAAA AGATCTACTG GGGCTTTGCT CAACCCTTTT
    TCAGTGCACA CCCCTAAGGC AATTCCTTCA GGAAAACCTG CTTCCCCTGT CCTCAGGAAT
    GAGCCTCTGT TAACTCCCAT TCCTCTTAAA CGTGCTGAAC TTGCTGGAGA GCTGGCTCAG
    CCAGAATGTC CAGAAGATGA GCAGGAGCTA GGGGTAATGG AGTTTGAAGA CGGTGACTTT
    GATGAGTCCA TGGACACAGA GAAGGTAGAT GAGAAGCCTG TGACTGCTAA GACTTGGGAC
    CAAGAGACTG AGCCAGTAGA AAGGGTGGAA CATGAGGCAG ATCCTGAGAG GGGAACCACA
    TCTTACTTAG AAAATTTTCT GCCAGATGTC TCTTGTTGGG ACATTGATCA GGATGATGAA
    AGCATACCTC AGGAAGTTCA AGTGGATTCC AGTAATCTCC CATTGGTAAA AGGGGCAGAT
    GATGAACAAG TGTTTCAGTT TTATTGGCTG GATGCTTATG AAGATCCATA CAACCAACCA
    GGTGTGGTTT TTCTGTTTGG CAAAGTGTGG ATTGAATCAG TCAAAACCCA TGTGAGCTGT
    TGTGTTATGG TGAAAAATAT TGAGCGAACA CTCTACTTCC TTCCCCGTGA AATGAAATTT
    GACCTAAACA CAGGGAAAGA AACAGCAATT CCAGTTACAA TGAAGGATGT TTATGAAGAA
    TTTGATTCAA AAATTTCAGC AAAATATAAA ATAATGAAGT TCAAGTCCAA GATAGTGGAA
    AAGAACTATG CTTTTGAGAT ACCTGATGTT CCAGAAAAAT CTGAGTACCT GGAAGTTAGA
    TACTCGGCAG AAGTGCCACA GCTTCCACAG AATTTAAAAG GAGAAACATT TTCTCATGTG
    TTTGGAACTA ACACGTCTAG CTTGGAACTG TTCTTGATGA ATAGGAAGAT CAAAGGACCT
    TGTTGGCTTG AAGTAAAAAA TCCACAACTC TTGAATCAGC CAATCAGTTG GTGTAAATTT
    GAGGTAATGG CTTTGAAACC AGACCTGGTG AATGTAATTA AGGATGTCAG TCCTCCTCCA
    CTTGTGGTGA TGTCTTTCAG CATGAAGACA ATGCAGAATG TACAGAACCA TCAACATGAG
    ATTATTGCTA TGGCGGCTTT GGTTCATCAC AGTTTTGCAT TAGATAAAGC ACCTCCAGAG
    CCTCCCTTTC AGACACATTT CTGTGTTGTG TCTAAACCAA AGGACTGTAT TTTCCCATGT
    GATTTCAAAG AAGTGATTTC GAAAAAGAAT ATGAAGGTTG AGATCGCTGC AACAGAAAGA
    ACCTTGATAG GATTTTTCCT TGCAAAAGTT CACAAAATTG ACCCTGACAT TCTTGTGGGT
    CATAATATTT GTAGTTTTGA ACTGGAAGTG CTGCTGCAAA GAATTAATGA GTGCAAGGTT
    CCTTACTGGT CCAAGATAGG TCGGCTGAGG AGATCCAACA TGCCAAAACT TGGGAGCCGG
    AGTGGATTTG GTGAAAGAAA TGCTACCTGT GGCCGAATGA TATGTGATGT GGAAATTTCA
    GCAAAAGAAT TGATTCATTG TAAAAGCTAC CATTTGTCTG AACTTGTTCA GCAGATTCTG
    AAAACTGAGA GGATTGTAAT TCCAACAGAA AATATACGAA ACATGTATAG TGAATCTTCT
    TATCTGTTGT ACCTGTTGGA ACATATCTGG AAAGATGCCA GATTCATTTT GCAGATCATG
    TGTGAGCTAA ATGTTCTTCC ATTAGCATTG CAGATCACTA ACATCGCTGG GAACATTATG
    TCTAGGACAC TGATGGGTGG ACGATCTGAG CGAAATGAGT TTTTGTTGCT TCATGCATTT
    TATGAAAACA ACTATATTGT GCCTGACAAG CAGATTTTCA GAAAACCTCA GCAAAAACTG
    GGAGATGAAG ATGAGGAAAT TGATGGGGAC ACCAATAAAT ACAAGAAAGG GCGTAAGAAA
    GCAACTTACG CTGGAGGCTT GGTTTTGGAC CCCAAAGTTG GTTTTTATGA CAAGTTCATT
    TTGCTCCTGG ATTTCAACAG TTTATATCCT TCCATCATTC AGGAATTTAA CATTTGTTTT
    ACAACAGTGC AAAGAGTTAC TTCAGAGGTG CAGAAAGCTA CAGAGGATGA GGAACAAGAA
    CAGATCCCTG AGTTGCCAGA TCCAAACTTA GAGATGGGCA TTTTGCCCAG AGAGATTCGG
    AAACTGGTAG AGCGGAGAAA ACAAGTCAAA CAGCTAATGA AACAGCAAGA TTTAAATCCA
    GACCTTGTTC TTCAGTATGA CATCCGACAG AAGGCTTTGA AGCTCACAGC AAACAGTATG
    TATGGTTGCC TGGGATTTTC GTACAGCAGA TTTTATGCTA AACCACTGGC TGCCTTGGTG
    ACATACAAAG GAAGGGAGAT TTTGATGCAT ACGAAAGACA TGGTACAGAA GATGAATCTT
    GAAGTTATTT ATGGAGACAC AGATTCAATT ATGATAAATA CCAATAGCAC CAACCTGGAA
    GAAGTATTTA AGTTGGGAAA CAAGGTAAAA AGTGAAGTGA ATAAATTGTA CAAACTGCTT
    GAAATAGACA TTGATGCTGT TTTCAAGTCT CTGCTGTTGC TGAAGAAAAA GAAATATGCT
    GCTCTAGTTG TTGAACCAAC ATCAGATGGG AATTACATTA CCAAGCAGGA GCTGAAAGGA
    TTAGATATAG TTAGAAGAGA CTGGTGTGAT CTTGCCAAGG ACACTGGAAA CTTTGTCATT
    GGCCAGATTC TTTCTGATCA AAGCCGGGAC ACCATTGTGG AAAACATTCA GAAGAGGTTA
    ATAGAAATTG GAGAAAATGT GCTAAATGGC AGTGTCCCAG TGAGCCAGTT TGAAATTAAC
    AAGGCATTGA CAAAGGATCC CCAGGATTAC CCTGATAGAA AAAGCTTACC TCATGTACAT
    GTTGCACTGT GGATAAATTC TCAAGGAGGC AGGAAGGTGA AAGCTGGTGA TACGGTGTCC
    TATGTTATTT GTCAGGATGG GTCAAACCTC ACTGCAACTC AGAGGGCCTA TGCCCCAGAA
    CAATTGCAGA AACTTGACAA TTTAGCCATC GACACCCAAT ACTACTTGGC CCAGCAGATC
    CACCCTGTTG TGGCTCGGAT CTGTGAGCCA ATAGATGGAA TTGATGCTGT ACTCATTGCA
    TTATGGCTAG GACTTGACTC CACTCAATTT AGAGTTCATC AGTATCACAA AGATGAAGAG
    AATGATGCCC TCCTTGGTGG TCCAGCACAG CTAACTGATG AAGAGAAATA CAAGGATTGT
    GAAAAATTCA AATGCCTTTG TCCTTCATGT GGAACTGAAA ATATTTATGA TAATGTCTTT
    GAAGGCTCGG GACTGGATAT GGAACCCAGT TTGTATCGTT GCAGTAATGT TGATTGTAAG
    GTTTCACCGT TGACCTTCAT GGTACAGCTG AGCAACAAAT TGATCATGGA CATTAGACGG
    TGCATTAAAA AGTACTATGA TGGCTGGTTG ATATGTGAAG AGCCAACTTG TTGCAGTCGC
    CTTCGACGTC TTCCCCTTCA TTTCTCCCGA AATGGGCCTC TGTGTCCAGT CTGCATGAAA
    GCTGTGCTTA GACCAGAGAT AAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.