asap1 gene cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

mRNA	Protein	Name
XM_012964135.2	XP_012819589.1	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X5
XM_012964138.2	XP_012819592.1	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X8
XM_012964132.2	XP_012819586.1	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X2
XM_031903142.1	XP_031759002.1	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X1
XM_012964134.3	XP_012819588.2	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X4
XM_031903144.1	XP_031759004.1	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X5
XM_012964138.3	XP_012819592.2	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X7
XM_012964131.2	XP_012819585.1	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X1
XM_012964136.2	XP_012819590.1	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X6
NM_001097266.1	NP_001090735.1	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1
XM_031903145.1	XP_031759005.1	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X6
XM_012964133.2	XP_012819587.1	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X3
XM_012964134.2	XP_012819588.1	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X4
XM_012964137.2	XP_012819591.1	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X7
XM_031903143.1	XP_031759003.1	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X2
XM_012964133.3	XP_012819587.2	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa59451	XM_031903142.1 Latest version!	Xenopus tropicalis ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (asap1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa59452	XM_031903143.1 Latest version!	Xenopus tropicalis ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (asap1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa59453	XM_012964133.3 Latest version!	Xenopus tropicalis ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (asap1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa59454	XM_012964134.3 Latest version!	Xenopus tropicalis ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (asap1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa59455	XM_031903144.1 Latest version!	Xenopus tropicalis ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (asap1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa59456	XM_031903145.1 Latest version!	Xenopus tropicalis ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (asap1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa59457	XM_012964138.3 Latest version!	Xenopus tropicalis ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (asap1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa23751	NM_001097266.1 Latest version!	Xenopus tropicalis ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (asap1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa29912	XM_012964131.2 Latest version!	Xenopus tropicalis ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (asap1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa29913	XM_012964132.2 Latest version!	Xenopus tropicalis ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (asap1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa29914	XM_012964133.2 Latest version!	Xenopus tropicalis ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (asap1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa29915	XM_012964134.2 Latest version!	Xenopus tropicalis ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (asap1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa29916	XM_012964135.2 Latest version!	Xenopus tropicalis ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (asap1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa29917	XM_012964136.2 Latest version!	Xenopus tropicalis ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (asap1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa29918	XM_012964137.2 Latest version!	Xenopus tropicalis ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (asap1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa29919	XM_012964138.2 Latest version!	Xenopus tropicalis ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (asap1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

asap1 ( XM_031903142.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031759002.1 Xenopus tropicalis ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (asap1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa59451
Clone ID Related Accession (Same CDS sequence)	XM_031903142.1
Accession Version	XM_031903142.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGATCTT CGGCCTCTAG ACTTTCGAGT TTTTCCTCTA GAGACTCACT ATGGAACCGG 
ATGCCCGATC AGATCTCAGT ATCGGAGTTT ATATCGGAGA CCACAGAGGA TTACAATTCG 
CCAACCACGT CCAGTTTCAC GACCCGTCTT CAGAGCTGCA GGAATTCGAT CAATGTGCTG 
GAAGAGGCCT TAGATCAAGA TAGGACAGCG TTGCAGAAAG TGAAAAAATC GGTGAAAGCA 
ATTTATAATT CAGGCCAAGA TCATGTCCAA AATGAAGAAA ACTATGCACA AGCCTTAGAT 
AAATTTGGAA GTAATTTTCT AAGTCGGGAT AACGCAGATT TGGGCACCGC CTTCGTGAAA 
TTCTCCACTC TAACAAAAGA ACTATCAACG CTTCTTAAAA ACCTGCTACA GGGATTAAGC 
CACAATGTCA TCTTCACCTT GGATTCTCTC TTGAAAGGGG ACTTAAAGGG CGTAAAAGGG 
GACCTTAAGA AGCCATTCGA CAGAGCGTGG AAGGATTATG AAACCAAATT CACGAAAATC 
GAAAAGGAGA AAAGGGAACA CGCCAAGCAA CATGGGATGA TCCGTACGGA GATTACTGGG 
GCCGAGATTG CCGAAGAGAT GGAGAAGGAG AGGAGATTAT TCCAGCTACA GATGTGTGAG 
TATCTCATTA AAGTTAATGA AATCAAGACT AAGAAAGGCG TGGATCTGCT GCAGAACCTT 
ATAAAGTACT ATCACGCACA GTGCAATTTC TTCCAGGATG GATTGAAAAC AGCTGACAAG 
CTGAAGCAGT ACATAGAGAA ATTGGCTGCA GATTTGTACA ATATTAAGCA GACCCAAGAT 
GAGGAGAAGA AACAGCTCAC TGCCCTGCGA GACCTGATAA AATCCTCTTT ACAGCTGGAT 
CAGAAGGAGT CTAGAAGAGA TTCCCAGAGT AAGCAGGCAG GCTACAGCAT GCACCAGCTC 
CAGGGGAACA AGGAGTATGG CAGCGAGAAG AAAGGCTACC TGCTGAAAAA AAGTGATGGC 
ATCCGGAAAG TATGGCAGAG GAGGAAGTGC ACTGTGAAAA ATGGAATCCT GACCATTTCC 
CATGCAACGT CAAACAGGCC ACCGACAAAG CTGAACCTGC TGACCTGCCA AGTCAAGCCG 
AACGCTGATG AGAAGAAATG CTTTGATCTC ATCTCACACA ACAGAACATA CCACTTCCAA 
GCAGAGGACG AACAAGAATA CATTGCGTGG ATATCAGTTC TGACAAATAG CAAAGAAGAT 
GCACTCAATA TGGCTTTCCG GGGAGACCAG AGCACTGGCG AGAGCAGCCT GGAGGATCTG 
ACTAAGGCCA TTATAGACGA TGTACAGAAA ACGCCTGGAA ATGAAGTTTG CTGTGATTGT 
GGGTCACCGG ACCCGACATG GCTTTCCACA AACCTTGGGA TTTTAACCTG TATAGAATGT 
TCGGGCATAC ATAGGGAAAT GGGGGTTCAT ATCTCTAGAA TCCAATCTCT GGAATTAGAC 
AAATTAGGGA CTTCTGAACT CTTGCTGGCC AAGAATGTAG GAAACAATAG CTTCAATGAT 
ATTATGGAAG GGAATTTGCT GTCTCCGTCA GCCAAGCCTT CTCCCTCCAG TGATATGATT 
GCACGTAAAG AATATATCAC TGCCAAGTAC GTGGAGCGGA GGTTTTCCAG AAAGATCTGT 
TCTACTGGGG CAGAAAAACT GAATGAGCTA CTTGAGGCTG TGAAATCCAG GGACTTACTT 
GCACTAATCC AAGTCTATGC AGAAGGTGTA GAGATCATGG AACCACTGCT AGAATCTGGA 
CAGGAGCCAG GTGAAACTGC ACTTCATTAT GCAGTCCGGA CAGCTGACCA CACGTCTCTT 
CCCTTGGTGG ACTTCCTGGT ACAAAACTGT GGAAATTTAG ATAAGCAGAC TGGAAAGGGC 
AACACTGCTT TACACTACTG CTGCTTATAT AACAAGCCGG AATGCTTAAA ATTGCTTTTG 
AGGGGAAAGC CAACTATTGA TGCTGTTAAC CATATTGGTG AGACGGCTTT TGATGTAGCA 
AAGAGACAGA AAGCAGCCCA GTGTGAGGAA CTGGTATCCC AAGCCCTGGC AGGGAAGCTG 
AATCCTCATG TTCACGTGGA GTACGACTGG AACCTGCGTC AGGAAGAAAT GGATGAGAGC 
GACGATGATT TGGAGGATAA GCCAAGCCCA ATAAAGAAAG AGCGTTCCCC CAGACCCCAG 
AGCTTCTGCC ATTCGTCCAG CATTTCCCCA CAAGACAAGC TGTCGCTGCC AGGTTTCAGT 
ACTCCTCGGG ACAAGCAGAG ACTCTCTTAT GGCGCCTTTA CCAACCAAAT ATTCATGTCT 
ACAAGCACAG ATTCCCCCAC GTCTCCTACT GCAGATGCCC CTCCGCTGCC CCCTAGGAAC 
GCAGGCAAAG TGGCGAGTGG CCCTACTTCA ACCCTCCCTC TAAGCACCCA ATCCCCTAGT 
GGCAGTTCCA CTCTGTCCAA GAAGAGACCT CCTCCCCCAC CCCCGGGACA CAAACGAACC 
CTCTCCGACC CACCAAGTCC GCTACCTCAT GGCCCCCCCA GTAAGGGCTC AGTTGTTTGT 
GGTAATGACT TGGGCCCGAG TGCTGCAGAC AGAACTCTAA ATAAGTTTGA GAGATTGTCA 
CAGTCATCAG GCACAGGAAC AACAAAGACT GCATTAGGCC CAAGAGTCCT ACCAAAACTA 
CCTCAGAAAG TGGCACTTAG GAAAATAGAC ACCACTCATC ATCTCTCAAT GGATAAGTAT 
AACCAGCAAC CCGAAATCTT CCAGAAATCT CCCCAGCCTG GAGATCTGCC TCAGAAGCCA 
CAAATTGCAG ATCTTCCTCC GAAACCGCAG CCTTTGGATA TCGCCCAGAA ACCACACGTC 
GGAGATCTAC CTCCCAAGCC CGGAGAATTG CCTCCTAAAC CTCAGCTAGG AGATTTGCCC 
CCGAAACCGC AAGATTTACC TCCAAAGCCA CAGCTGAAAG ATCTTCCGCC CAAGCCCAAC 
CTGGCAGAGG TGATGTGCAA AACTCCTGTT GCGGATATAG TCCCCAAACC TCATACCAAT 
GAGGCCAATC AAAATTCCCA GCCGGTGGAT GCGACCCATA ATGCACAGCC CACGGACCAA 
ACCCCTGTCC AGAAGCAAGT TCCAGAAACT TCCAATAATG GCCCACCGCC TCTTCCAGAA 
ACACCCGTGC CACTTCCCCG AAAGATAAAC ACAGGGAAGA ATAAGCTTAA ACGAGTTAAA 
ACAATATACG ACTGCCAAGC GGACAATGAT GATGAACTCA CATTTGCGGA GGGGGAAGTG 
ATTATCGTCA CTGGAGAGGA GGACCAGGAG TGGTGGATTG GGCACATCGA AGGCTTCCCG 
GACAGGAAAG GAGTATTCCC TGTGTCCTTC GTCCACCTGT TGACTGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031759002.1
CDS	313..3723
Translation	MRSSASRLSSFSSRDSLWNRMPDQISVSEFISETTEDYNSPTTSSFTTRLQSCRNSINVLEEALDQDRTALQKVKKSVKAIYNSGQDHVQNEENYAQALDKFGSNFLSRDNADLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGVKGDLKKPFDRAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDQKESRRDSQSKQAGYSMHQLQGNKEYGSEKKGYLLKKSDGIRKVWQRRKCTVKNGILTISHATSNRPPTKLNLLTCQVKPNADEKKCFDLISHNRTYHFQAEDEQEYIAWISVLTNSKEDALNMAFRGDQSTGESSLEDLTKAIIDDVQKTPGNEVCCDCGSPDPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDKLGTSELLLAKNVGNNSFNDIMEGNLLSPSAKPSPSSDMIARKEYITAKYVERRFSRKICSTGAEKLNELLEAVKSRDLLALIQVYAEGVEIMEPLLESGQEPGETALHYAVRTADHTSLPLVDFLVQNCGNLDKQTGKGNTALHYCCLYNKPECLKLLLRGKPTIDAVNHIGETAFDVAKRQKAAQCEELVSQALAGKLNPHVHVEYDWNLRQEEMDESDDDLEDKPSPIKKERSPRPQSFCHSSSISPQDKLSLPGFSTPRDKQRLSYGAFTNQIFMSTSTDSPTSPTADAPPLPPRNAGKVASGPTSTLPLSTQSPSGSSTLSKKRPPPPPPGHKRTLSDPPSPLPHGPPSKGSVVCGNDLGPSAADRTLNKFERLSQSSGTGTTKTALGPRVLPKLPQKVALRKIDTTHHLSMDKYNQQPEIFQKSPQPGDLPQKPQIADLPPKPQPLDIAQKPHVGDLPPKPGELPPKPQLGDLPPKPQDLPPKPQLKDLPPKPNLAEVMCKTPVADIVPKPHTNEANQNSQPVDATHNAQPTDQTPVQKQVPETSNNGPPPLPETPVPLPRKINTGKNKLKRVKTIYDCQADNDDELTFAEGEVIIVTGEEDQEWWIGHIEGFPDRKGVFPVSFVHLLTE

Target ORF information:

RefSeq Version	XM_031903142.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (asap1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031903142.1

ORF Insert Sequence:

ATGCGATCTT CGGCCTCTAG ACTTTCGAGT TTTTCCTCTA GAGACTCACT ATGGAACCGG 
ATGCCCGATC AGATCTCAGT ATCGGAGTTT ATATCGGAGA CCACAGAGGA TTACAATTCG 
CCAACCACGT CCAGTTTCAC GACCCGTCTT CAGAGCTGCA GGAATTCGAT CAATGTGCTG 
GAAGAGGCCT TAGATCAAGA TAGGACAGCG TTGCAGAAAG TGAAAAAATC GGTGAAAGCA 
ATTTATAATT CAGGCCAAGA TCATGTCCAA AATGAAGAAA ACTATGCACA AGCCTTAGAT 
AAATTTGGAA GTAATTTTCT AAGTCGGGAT AACGCAGATT TGGGCACCGC CTTCGTGAAA 
TTCTCCACTC TAACAAAAGA ACTATCAACG CTTCTTAAAA ACCTGCTACA GGGATTAAGC 
CACAATGTCA TCTTCACCTT GGATTCTCTC TTGAAAGGGG ACTTAAAGGG CGTAAAAGGG 
GACCTTAAGA AGCCATTCGA CAGAGCGTGG AAGGATTATG AAACCAAATT CACGAAAATC 
GAAAAGGAGA AAAGGGAACA CGCCAAGCAA CATGGGATGA TCCGTACGGA GATTACTGGG 
GCCGAGATTG CCGAAGAGAT GGAGAAGGAG AGGAGATTAT TCCAGCTACA GATGTGTGAG 
TATCTCATTA AAGTTAATGA AATCAAGACT AAGAAAGGCG TGGATCTGCT GCAGAACCTT 
ATAAAGTACT ATCACGCACA GTGCAATTTC TTCCAGGATG GATTGAAAAC AGCTGACAAG 
CTGAAGCAGT ACATAGAGAA ATTGGCTGCA GATTTGTACA ATATTAAGCA GACCCAAGAT 
GAGGAGAAGA AACAGCTCAC TGCCCTGCGA GACCTGATAA AATCCTCTTT ACAGCTGGAT 
CAGAAGGAGT CTAGAAGAGA TTCCCAGAGT AAGCAGGCAG GCTACAGCAT GCACCAGCTC 
CAGGGGAACA AGGAGTATGG CAGCGAGAAG AAAGGCTACC TGCTGAAAAA AAGTGATGGC 
ATCCGGAAAG TATGGCAGAG GAGGAAGTGC ACTGTGAAAA ATGGAATCCT GACCATTTCC 
CATGCAACGT CAAACAGGCC ACCGACAAAG CTGAACCTGC TGACCTGCCA AGTCAAGCCG 
AACGCTGATG AGAAGAAATG CTTTGATCTC ATCTCACACA ACAGAACATA CCACTTCCAA 
GCAGAGGACG AACAAGAATA CATTGCGTGG ATATCAGTTC TGACAAATAG CAAAGAAGAT 
GCACTCAATA TGGCTTTCCG GGGAGACCAG AGCACTGGCG AGAGCAGCCT GGAGGATCTG 
ACTAAGGCCA TTATAGACGA TGTACAGAAA ACGCCTGGAA ATGAAGTTTG CTGTGATTGT 
GGGTCACCGG ACCCGACATG GCTTTCCACA AACCTTGGGA TTTTAACCTG TATAGAATGT 
TCGGGCATAC ATAGGGAAAT GGGGGTTCAT ATCTCTAGAA TCCAATCTCT GGAATTAGAC 
AAATTAGGGA CTTCTGAACT CTTGCTGGCC AAGAATGTAG GAAACAATAG CTTCAATGAT 
ATTATGGAAG GGAATTTGCT GTCTCCGTCA GCCAAGCCTT CTCCCTCCAG TGATATGATT 
GCACGTAAAG AATATATCAC TGCCAAGTAC GTGGAGCGGA GGTTTTCCAG AAAGATCTGT 
TCTACTGGGG CAGAAAAACT GAATGAGCTA CTTGAGGCTG TGAAATCCAG GGACTTACTT 
GCACTAATCC AAGTCTATGC AGAAGGTGTA GAGATCATGG AACCACTGCT AGAATCTGGA 
CAGGAGCCAG GTGAAACTGC ACTTCATTAT GCAGTCCGGA CAGCTGACCA CACGTCTCTT 
CCCTTGGTGG ACTTCCTGGT ACAAAACTGT GGAAATTTAG ATAAGCAGAC TGGAAAGGGC 
AACACTGCTT TACACTACTG CTGCTTATAT AACAAGCCGG AATGCTTAAA ATTGCTTTTG 
AGGGGAAAGC CAACTATTGA TGCTGTTAAC CATATTGGTG AGACGGCTTT TGATGTAGCA 
AAGAGACAGA AAGCAGCCCA GTGTGAGGAA CTGGTATCCC AAGCCCTGGC AGGGAAGCTG 
AATCCTCATG TTCACGTGGA GTACGACTGG AACCTGCGTC AGGAAGAAAT GGATGAGAGC 
GACGATGATT TGGAGGATAA GCCAAGCCCA ATAAAGAAAG AGCGTTCCCC CAGACCCCAG 
AGCTTCTGCC ATTCGTCCAG CATTTCCCCA CAAGACAAGC TGTCGCTGCC AGGTTTCAGT 
ACTCCTCGGG ACAAGCAGAG ACTCTCTTAT GGCGCCTTTA CCAACCAAAT ATTCATGTCT 
ACAAGCACAG ATTCCCCCAC GTCTCCTACT GCAGATGCCC CTCCGCTGCC CCCTAGGAAC 
GCAGGCAAAG TGGCGAGTGG CCCTACTTCA ACCCTCCCTC TAAGCACCCA ATCCCCTAGT 
GGCAGTTCCA CTCTGTCCAA GAAGAGACCT CCTCCCCCAC CCCCGGGACA CAAACGAACC 
CTCTCCGACC CACCAAGTCC GCTACCTCAT GGCCCCCCCA GTAAGGGCTC AGTTGTTTGT 
GGTAATGACT TGGGCCCGAG TGCTGCAGAC AGAACTCTAA ATAAGTTTGA GAGATTGTCA 
CAGTCATCAG GCACAGGAAC AACAAAGACT GCATTAGGCC CAAGAGTCCT ACCAAAACTA 
CCTCAGAAAG TGGCACTTAG GAAAATAGAC ACCACTCATC ATCTCTCAAT GGATAAGTAT 
AACCAGCAAC CCGAAATCTT CCAGAAATCT CCCCAGCCTG GAGATCTGCC TCAGAAGCCA 
CAAATTGCAG ATCTTCCTCC GAAACCGCAG CCTTTGGATA TCGCCCAGAA ACCACACGTC 
GGAGATCTAC CTCCCAAGCC CGGAGAATTG CCTCCTAAAC CTCAGCTAGG AGATTTGCCC 
CCGAAACCGC AAGATTTACC TCCAAAGCCA CAGCTGAAAG ATCTTCCGCC CAAGCCCAAC 
CTGGCAGAGG TGATGTGCAA AACTCCTGTT GCGGATATAG TCCCCAAACC TCATACCAAT 
GAGGCCAATC AAAATTCCCA GCCGGTGGAT GCGACCCATA ATGCACAGCC CACGGACCAA 
ACCCCTGTCC AGAAGCAAGT TCCAGAAACT TCCAATAATG GCCCACCGCC TCTTCCAGAA 
ACACCCGTGC CACTTCCCCG AAAGATAAAC ACAGGGAAGA ATAAGCTTAA ACGAGTTAAA 
ACAATATACG ACTGCCAAGC GGACAATGAT GATGAACTCA CATTTGCGGA GGGGGAAGTG 
ATTATCGTCA CTGGAGAGGA GGACCAGGAG TGGTGGATTG GGCACATCGA AGGCTTCCCG 
GACAGGAAAG GAGTATTCCC TGTGTCCTTC GTCCACCTGT TGACTGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

asap1 ( XM_031903143.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031759003.1 Xenopus tropicalis ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (asap1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa59452
Clone ID Related Accession (Same CDS sequence)	XM_031903143.1
Accession Version	XM_031903143.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3402bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGATCTT CGGCCTCTAG ACTTTCGAGT TTTTCCTCTA GAGACTCACT ATGGAACCGG 
ATGCCCGATC AGATCTCAGT ATCGGAGTTT ATATCGGAGA CCACAGAGGA TTACAATTCG 
CCAACCACGT CCAGTTTCAC GACCCGTCTT CAGAGCTGCA GGAATTCGAT CAATGTGCTG 
GAAGAGGCCT TAGATCAAGA TAGGACAGCG TTGCAGAAAG TGAAAAAATC GGTGAAAGCA 
ATTTATAATT CAGGCCAAGA TCATGTCCAA AATGAAGAAA ACTATGCACA AGCCTTAGAT 
AAATTTGGAA GTAATTTTCT AAGTCGGGAT AACGCAGATT TGGGCACCGC CTTCGTGAAA 
TTCTCCACTC TAACAAAAGA ACTATCAACG CTTCTTAAAA ACCTGCTACA GGGATTAAGC 
CACAATGTCA TCTTCACCTT GGATTCTCTC TTGAAAGGGG ACTTAAAGGG CGTAAAAGGG 
GACCTTAAGA AGCCATTCGA CAGAGCGTGG AAGGATTATG AAACCAAATT CACGAAAATC 
GAAAAGGAGA AAAGGGAACA CGCCAAGCAA CATGGGATGA TCCGTACGGA GATTACTGGG 
GCCGAGATTG CCGAAGAGAT GGAGAAGGAG AGGAGATTAT TCCAGCTACA GATGTGTGAG 
TATCTCATTA AAGTTAATGA AATCAAGACT AAGAAAGGCG TGGATCTGCT GCAGAACCTT 
ATAAAGTACT ATCACGCACA GTGCAATTTC TTCCAGGATG GATTGAAAAC AGCTGACAAG 
CTGAAGCAGT ACATAGAGAA ATTGGCTGCA GATTTGTACA ATATTAAGCA GACCCAAGAT 
GAGGAGAAGA AACAGCTCAC TGCCCTGCGA GACCTGATAA AATCCTCTTT ACAGCTGGAT 
CAGAAGGAGG ATTCCCAGAG TAAGCAGGCA GGCTACAGCA TGCACCAGCT CCAGGGGAAC 
AAGGAGTATG GCAGCGAGAA GAAAGGCTAC CTGCTGAAAA AAAGTGATGG CATCCGGAAA 
GTATGGCAGA GGAGGAAGTG CACTGTGAAA AATGGAATCC TGACCATTTC CCATGCAACG 
TCAAACAGGC CACCGACAAA GCTGAACCTG CTGACCTGCC AAGTCAAGCC GAACGCTGAT 
GAGAAGAAAT GCTTTGATCT CATCTCACAC AACAGAACAT ACCACTTCCA AGCAGAGGAC 
GAACAAGAAT ACATTGCGTG GATATCAGTT CTGACAAATA GCAAAGAAGA TGCACTCAAT 
ATGGCTTTCC GGGGAGACCA GAGCACTGGC GAGAGCAGCC TGGAGGATCT GACTAAGGCC 
ATTATAGACG ATGTACAGAA AACGCCTGGA AATGAAGTTT GCTGTGATTG TGGGTCACCG 
GACCCGACAT GGCTTTCCAC AAACCTTGGG ATTTTAACCT GTATAGAATG TTCGGGCATA 
CATAGGGAAA TGGGGGTTCA TATCTCTAGA ATCCAATCTC TGGAATTAGA CAAATTAGGG 
ACTTCTGAAC TCTTGCTGGC CAAGAATGTA GGAAACAATA GCTTCAATGA TATTATGGAA 
GGGAATTTGC TGTCTCCGTC AGCCAAGCCT TCTCCCTCCA GTGATATGAT TGCACGTAAA 
GAATATATCA CTGCCAAGTA CGTGGAGCGG AGGTTTTCCA GAAAGATCTG TTCTACTGGG 
GCAGAAAAAC TGAATGAGCT ACTTGAGGCT GTGAAATCCA GGGACTTACT TGCACTAATC 
CAAGTCTATG CAGAAGGTGT AGAGATCATG GAACCACTGC TAGAATCTGG ACAGGAGCCA 
GGTGAAACTG CACTTCATTA TGCAGTCCGG ACAGCTGACC ACACGTCTCT TCCCTTGGTG 
GACTTCCTGG TACAAAACTG TGGAAATTTA GATAAGCAGA CTGGAAAGGG CAACACTGCT 
TTACACTACT GCTGCTTATA TAACAAGCCG GAATGCTTAA AATTGCTTTT GAGGGGAAAG 
CCAACTATTG ATGCTGTTAA CCATATTGGT GAGACGGCTT TTGATGTAGC AAAGAGACAG 
AAAGCAGCCC AGTGTGAGGA ACTGGTATCC CAAGCCCTGG CAGGGAAGCT GAATCCTCAT 
GTTCACGTGG AGTACGACTG GAACCTGCGT CAGGAAGAAA TGGATGAGAG CGACGATGAT 
TTGGAGGATA AGCCAAGCCC AATAAAGAAA GAGCGTTCCC CCAGACCCCA GAGCTTCTGC 
CATTCGTCCA GCATTTCCCC ACAAGACAAG CTGTCGCTGC CAGGTTTCAG TACTCCTCGG 
GACAAGCAGA GACTCTCTTA TGGCGCCTTT ACCAACCAAA TATTCATGTC TACAAGCACA 
GATTCCCCCA CGTCTCCTAC TGCAGATGCC CCTCCGCTGC CCCCTAGGAA CGCAGGCAAA 
GTGGCGAGTG GCCCTACTTC AACCCTCCCT CTAAGCACCC AATCCCCTAG TGGCAGTTCC 
ACTCTGTCCA AGAAGAGACC TCCTCCCCCA CCCCCGGGAC ACAAACGAAC CCTCTCCGAC 
CCACCAAGTC CGCTACCTCA TGGCCCCCCC AGTAAGGGCT CAGTTGTTTG TGGTAATGAC 
TTGGGCCCGA GTGCTGCAGA CAGAACTCTA AATAAGTTTG AGAGATTGTC ACAGTCATCA 
GGCACAGGAA CAACAAAGAC TGCATTAGGC CCAAGAGTCC TACCAAAACT ACCTCAGAAA 
GTGGCACTTA GGAAAATAGA CACCACTCAT CATCTCTCAA TGGATAAGTA TAACCAGCAA 
CCCGAAATCT TCCAGAAATC TCCCCAGCCT GGAGATCTGC CTCAGAAGCC ACAAATTGCA 
GATCTTCCTC CGAAACCGCA GCCTTTGGAT ATCGCCCAGA AACCACACGT CGGAGATCTA 
CCTCCCAAGC CCGGAGAATT GCCTCCTAAA CCTCAGCTAG GAGATTTGCC CCCGAAACCG 
CAAGATTTAC CTCCAAAGCC ACAGCTGAAA GATCTTCCGC CCAAGCCCAA CCTGGCAGAG 
GTGATGTGCA AAACTCCTGT TGCGGATATA GTCCCCAAAC CTCATACCAA TGAGGCCAAT 
CAAAATTCCC AGCCGGTGGA TGCGACCCAT AATGCACAGC CCACGGACCA AACCCCTGTC 
CAGAAGCAAG TTCCAGAAAC TTCCAATAAT GGCCCACCGC CTCTTCCAGA AACACCCGTG 
CCACTTCCCC GAAAGATAAA CACAGGGAAG AATAAGCTTA AACGAGTTAA AACAATATAC 
GACTGCCAAG CGGACAATGA TGATGAACTC ACATTTGCGG AGGGGGAAGT GATTATCGTC 
ACTGGAGAGG AGGACCAGGA GTGGTGGATT GGGCACATCG AAGGCTTCCC GGACAGGAAA 
GGAGTATTCC CTGTGTCCTT CGTCCACCTG TTGACTGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031759003.1
CDS	312..3713
Translation	MRSSASRLSSFSSRDSLWNRMPDQISVSEFISETTEDYNSPTTSSFTTRLQSCRNSINVLEEALDQDRTALQKVKKSVKAIYNSGQDHVQNEENYAQALDKFGSNFLSRDNADLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGVKGDLKKPFDRAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDQKEDSQSKQAGYSMHQLQGNKEYGSEKKGYLLKKSDGIRKVWQRRKCTVKNGILTISHATSNRPPTKLNLLTCQVKPNADEKKCFDLISHNRTYHFQAEDEQEYIAWISVLTNSKEDALNMAFRGDQSTGESSLEDLTKAIIDDVQKTPGNEVCCDCGSPDPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDKLGTSELLLAKNVGNNSFNDIMEGNLLSPSAKPSPSSDMIARKEYITAKYVERRFSRKICSTGAEKLNELLEAVKSRDLLALIQVYAEGVEIMEPLLESGQEPGETALHYAVRTADHTSLPLVDFLVQNCGNLDKQTGKGNTALHYCCLYNKPECLKLLLRGKPTIDAVNHIGETAFDVAKRQKAAQCEELVSQALAGKLNPHVHVEYDWNLRQEEMDESDDDLEDKPSPIKKERSPRPQSFCHSSSISPQDKLSLPGFSTPRDKQRLSYGAFTNQIFMSTSTDSPTSPTADAPPLPPRNAGKVASGPTSTLPLSTQSPSGSSTLSKKRPPPPPPGHKRTLSDPPSPLPHGPPSKGSVVCGNDLGPSAADRTLNKFERLSQSSGTGTTKTALGPRVLPKLPQKVALRKIDTTHHLSMDKYNQQPEIFQKSPQPGDLPQKPQIADLPPKPQPLDIAQKPHVGDLPPKPGELPPKPQLGDLPPKPQDLPPKPQLKDLPPKPNLAEVMCKTPVADIVPKPHTNEANQNSQPVDATHNAQPTDQTPVQKQVPETSNNGPPPLPETPVPLPRKINTGKNKLKRVKTIYDCQADNDDELTFAEGEVIIVTGEEDQEWWIGHIEGFPDRKGVFPVSFVHLLTE

Target ORF information:

RefSeq Version	XM_031903143.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (asap1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031903143.1

ORF Insert Sequence:

ATGCGATCTT CGGCCTCTAG ACTTTCGAGT TTTTCCTCTA GAGACTCACT ATGGAACCGG 
ATGCCCGATC AGATCTCAGT ATCGGAGTTT ATATCGGAGA CCACAGAGGA TTACAATTCG 
CCAACCACGT CCAGTTTCAC GACCCGTCTT CAGAGCTGCA GGAATTCGAT CAATGTGCTG 
GAAGAGGCCT TAGATCAAGA TAGGACAGCG TTGCAGAAAG TGAAAAAATC GGTGAAAGCA 
ATTTATAATT CAGGCCAAGA TCATGTCCAA AATGAAGAAA ACTATGCACA AGCCTTAGAT 
AAATTTGGAA GTAATTTTCT AAGTCGGGAT AACGCAGATT TGGGCACCGC CTTCGTGAAA 
TTCTCCACTC TAACAAAAGA ACTATCAACG CTTCTTAAAA ACCTGCTACA GGGATTAAGC 
CACAATGTCA TCTTCACCTT GGATTCTCTC TTGAAAGGGG ACTTAAAGGG CGTAAAAGGG 
GACCTTAAGA AGCCATTCGA CAGAGCGTGG AAGGATTATG AAACCAAATT CACGAAAATC 
GAAAAGGAGA AAAGGGAACA CGCCAAGCAA CATGGGATGA TCCGTACGGA GATTACTGGG 
GCCGAGATTG CCGAAGAGAT GGAGAAGGAG AGGAGATTAT TCCAGCTACA GATGTGTGAG 
TATCTCATTA AAGTTAATGA AATCAAGACT AAGAAAGGCG TGGATCTGCT GCAGAACCTT 
ATAAAGTACT ATCACGCACA GTGCAATTTC TTCCAGGATG GATTGAAAAC AGCTGACAAG 
CTGAAGCAGT ACATAGAGAA ATTGGCTGCA GATTTGTACA ATATTAAGCA GACCCAAGAT 
GAGGAGAAGA AACAGCTCAC TGCCCTGCGA GACCTGATAA AATCCTCTTT ACAGCTGGAT 
CAGAAGGAGG ATTCCCAGAG TAAGCAGGCA GGCTACAGCA TGCACCAGCT CCAGGGGAAC 
AAGGAGTATG GCAGCGAGAA GAAAGGCTAC CTGCTGAAAA AAAGTGATGG CATCCGGAAA 
GTATGGCAGA GGAGGAAGTG CACTGTGAAA AATGGAATCC TGACCATTTC CCATGCAACG 
TCAAACAGGC CACCGACAAA GCTGAACCTG CTGACCTGCC AAGTCAAGCC GAACGCTGAT 
GAGAAGAAAT GCTTTGATCT CATCTCACAC AACAGAACAT ACCACTTCCA AGCAGAGGAC 
GAACAAGAAT ACATTGCGTG GATATCAGTT CTGACAAATA GCAAAGAAGA TGCACTCAAT 
ATGGCTTTCC GGGGAGACCA GAGCACTGGC GAGAGCAGCC TGGAGGATCT GACTAAGGCC 
ATTATAGACG ATGTACAGAA AACGCCTGGA AATGAAGTTT GCTGTGATTG TGGGTCACCG 
GACCCGACAT GGCTTTCCAC AAACCTTGGG ATTTTAACCT GTATAGAATG TTCGGGCATA 
CATAGGGAAA TGGGGGTTCA TATCTCTAGA ATCCAATCTC TGGAATTAGA CAAATTAGGG 
ACTTCTGAAC TCTTGCTGGC CAAGAATGTA GGAAACAATA GCTTCAATGA TATTATGGAA 
GGGAATTTGC TGTCTCCGTC AGCCAAGCCT TCTCCCTCCA GTGATATGAT TGCACGTAAA 
GAATATATCA CTGCCAAGTA CGTGGAGCGG AGGTTTTCCA GAAAGATCTG TTCTACTGGG 
GCAGAAAAAC TGAATGAGCT ACTTGAGGCT GTGAAATCCA GGGACTTACT TGCACTAATC 
CAAGTCTATG CAGAAGGTGT AGAGATCATG GAACCACTGC TAGAATCTGG ACAGGAGCCA 
GGTGAAACTG CACTTCATTA TGCAGTCCGG ACAGCTGACC ACACGTCTCT TCCCTTGGTG 
GACTTCCTGG TACAAAACTG TGGAAATTTA GATAAGCAGA CTGGAAAGGG CAACACTGCT 
TTACACTACT GCTGCTTATA TAACAAGCCG GAATGCTTAA AATTGCTTTT GAGGGGAAAG 
CCAACTATTG ATGCTGTTAA CCATATTGGT GAGACGGCTT TTGATGTAGC AAAGAGACAG 
AAAGCAGCCC AGTGTGAGGA ACTGGTATCC CAAGCCCTGG CAGGGAAGCT GAATCCTCAT 
GTTCACGTGG AGTACGACTG GAACCTGCGT CAGGAAGAAA TGGATGAGAG CGACGATGAT 
TTGGAGGATA AGCCAAGCCC AATAAAGAAA GAGCGTTCCC CCAGACCCCA GAGCTTCTGC 
CATTCGTCCA GCATTTCCCC ACAAGACAAG CTGTCGCTGC CAGGTTTCAG TACTCCTCGG 
GACAAGCAGA GACTCTCTTA TGGCGCCTTT ACCAACCAAA TATTCATGTC TACAAGCACA 
GATTCCCCCA CGTCTCCTAC TGCAGATGCC CCTCCGCTGC CCCCTAGGAA CGCAGGCAAA 
GTGGCGAGTG GCCCTACTTC AACCCTCCCT CTAAGCACCC AATCCCCTAG TGGCAGTTCC 
ACTCTGTCCA AGAAGAGACC TCCTCCCCCA CCCCCGGGAC ACAAACGAAC CCTCTCCGAC 
CCACCAAGTC CGCTACCTCA TGGCCCCCCC AGTAAGGGCT CAGTTGTTTG TGGTAATGAC 
TTGGGCCCGA GTGCTGCAGA CAGAACTCTA AATAAGTTTG AGAGATTGTC ACAGTCATCA 
GGCACAGGAA CAACAAAGAC TGCATTAGGC CCAAGAGTCC TACCAAAACT ACCTCAGAAA 
GTGGCACTTA GGAAAATAGA CACCACTCAT CATCTCTCAA TGGATAAGTA TAACCAGCAA 
CCCGAAATCT TCCAGAAATC TCCCCAGCCT GGAGATCTGC CTCAGAAGCC ACAAATTGCA 
GATCTTCCTC CGAAACCGCA GCCTTTGGAT ATCGCCCAGA AACCACACGT CGGAGATCTA 
CCTCCCAAGC CCGGAGAATT GCCTCCTAAA CCTCAGCTAG GAGATTTGCC CCCGAAACCG 
CAAGATTTAC CTCCAAAGCC ACAGCTGAAA GATCTTCCGC CCAAGCCCAA CCTGGCAGAG 
GTGATGTGCA AAACTCCTGT TGCGGATATA GTCCCCAAAC CTCATACCAA TGAGGCCAAT 
CAAAATTCCC AGCCGGTGGA TGCGACCCAT AATGCACAGC CCACGGACCA AACCCCTGTC 
CAGAAGCAAG TTCCAGAAAC TTCCAATAAT GGCCCACCGC CTCTTCCAGA AACACCCGTG 
CCACTTCCCC GAAAGATAAA CACAGGGAAG AATAAGCTTA AACGAGTTAA AACAATATAC 
GACTGCCAAG CGGACAATGA TGATGAACTC ACATTTGCGG AGGGGGAAGT GATTATCGTC 
ACTGGAGAGG AGGACCAGGA GTGGTGGATT GGGCACATCG AAGGCTTCCC GGACAGGAAA 
GGAGTATTCC CTGTGTCCTT CGTCCACCTG TTGACTGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

asap1 ( XM_012964133.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819587.2 Xenopus tropicalis ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (asap1), transcript variant X3, mRNA.

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CloneID	OXa59453
Clone ID Related Accession (Same CDS sequence)	XM_012964133.3
Accession Version	XM_012964133.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012964133.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCCCG ATCAGATCTC AGTATCGGAG TTTATATCGG AGACCACAGA GGATTACAAT 
TCGCCAACCA CGTCCAGTTT CACGACCCGT CTTCAGAGCT GCAGGAATTC GATCAATGTG 
CTGGAAGAGG CCTTAGATCA AGATAGGACA GCGTTGCAGA AAGTGAAAAA ATCGGTGAAA 
GCAATTTATA ATTCAGGCCA AGATCATGTC CAAAATGAAG AAAACTATGC ACAAGCCTTA 
GATAAATTTG GAAGTAATTT TCTAAGTCGG GATAACGCAG ATTTGGGCAC CGCCTTCGTG 
AAATTCTCCA CTCTAACAAA AGAACTATCA ACGCTTCTTA AAAACCTGCT ACAGGGATTA 
AGCCACAATG TCATCTTCAC CTTGGATTCT CTCTTGAAAG GGGACTTAAA GGGCGTAAAA 
GGGGACCTTA AGAAGCCATT CGACAGAGCG TGGAAGGATT ATGAAACCAA ATTCACGAAA 
ATCGAAAAGG AGAAAAGGGA ACACGCCAAG CAACATGGGA TGATCCGTAC GGAGATTACT 
GGGGCCGAGA TTGCCGAAGA GATGGAGAAG GAGAGGAGAT TATTCCAGCT ACAGATGTGT 
GAGTATCTCA TTAAAGTTAA TGAAATCAAG ACTAAGAAAG GCGTGGATCT GCTGCAGAAC 
CTTATAAAGT ACTATCACGC ACAGTGCAAT TTCTTCCAGG ATGGATTGAA AACAGCTGAC 
AAGCTGAAGC AGTACATAGA GAAATTGGCT GCAGATTTGT ACAATATTAA GCAGACCCAA 
GATGAGGAGA AGAAACAGCT CACTGCCCTG CGAGACCTGA TAAAATCCTC TTTACAGCTG 
GATCAGAAGG AGTCTAGAAG AGATTCCCAG AGTAAGCAGG CAGGCTACAG CATGCACCAG 
CTCCAGGGGA ACAAGGAGTA TGGCAGCGAG AAGAAAGGCT ACCTGCTGAA AAAAAGTGAT 
GGCATCCGGA AAGTATGGCA GAGGAGGAAG TGCACTGTGA AAAATGGAAT CCTGACCATT 
TCCCATGCAA CGTCAAACAG GCCACCGACA AAGCTGAACC TGCTGACCTG CCAAGTCAAG 
CCGAACGCTG ATGAGAAGAA ATGCTTTGAT CTCATCTCAC ACAACAGAAC ATACCACTTC 
CAAGCAGAGG ACGAACAAGA ATACATTGCG TGGATATCAG TTCTGACAAA TAGCAAAGAA 
GATGCACTCA ATATGGCTTT CCGGGGAGAC CAGAGCACTG GCGAGAGCAG CCTGGAGGAT 
CTGACTAAGG CCATTATAGA CGATGTACAG AAAACGCCTG GAAATGAAGT TTGCTGTGAT 
TGTGGGTCAC CGGACCCGAC ATGGCTTTCC ACAAACCTTG GGATTTTAAC CTGTATAGAA 
TGTTCGGGCA TACATAGGGA AATGGGGGTT CATATCTCTA GAATCCAATC TCTGGAATTA 
GACAAATTAG GGACTTCTGA ACTCTTGCTG GCCAAGAATG TAGGAAACAA TAGCTTCAAT 
GATATTATGG AAGGGAATTT GCTGTCTCCG TCAGCCAAGC CTTCTCCCTC CAGTGATATG 
ATTGCACGTA AAGAATATAT CACTGCCAAG TACGTGGAGC GGAGGTTTTC CAGAAAGATC 
TGTTCTACTG GGGCAGAAAA ACTGAATGAG CTACTTGAGG CTGTGAAATC CAGGGACTTA 
CTTGCACTAA TCCAAGTCTA TGCAGAAGGT GTAGAGATCA TGGAACCACT GCTAGAATCT 
GGACAGGAGC CAGGTGAAAC TGCACTTCAT TATGCAGTCC GGACAGCTGA CCACACGTCT 
CTTCCCTTGG TGGACTTCCT GGTACAAAAC TGTGGAAATT TAGATAAGCA GACTGGAAAG 
GGCAACACTG CTTTACACTA CTGCTGCTTA TATAACAAGC CGGAATGCTT AAAATTGCTT 
TTGAGGGGAA AGCCAACTAT TGATGCTGTT AACCATATTG GTGAGACGGC TTTTGATGTA 
GCAAAGAGAC AGAAAGCAGC CCAGTGTGAG GAACTGGTAT CCCAAGCCCT GGCAGGGAAG 
CTGAATCCTC ATGTTCACGT GGAGTACGAC TGGAACCTGC GTCAGGAAGA AATGGATGAG 
AGCGACGATG ATTTGGAGGA TAAGCCAAGC CCAATAAAGA AAGAGCGTTC CCCCAGACCC 
CAGAGCTTCT GCCATTCGTC CAGCATTTCC CCACAAGACA AGCTGTCGCT GCCAGGTTTC 
AGTACTCCTC GGGACAAGCA GAGACTCTCT TATGGCGCCT TTACCAACCA AATATTCATG 
TCTACAAGCA CAGATTCCCC CACGTCTCCT ACTGCAGATG CCCCTCCGCT GCCCCCTAGG 
AACGCAGGCA AAGTGGCGAG TGGCCCTACT TCAACCCTCC CTCTAAGCAC CCAATCCCCT 
AGTGGCAGTT CCACTCTGTC CAAGAAGAGA CCTCCTCCCC CACCCCCGGG ACACAAACGA 
ACCCTCTCCG ACCCACCAAG TCCGCTACCT CATGGCCCCC CCAGTAAGGG CTCAGTTGTT 
TGTGGTAATG ACTTGGGCCC GAGTGCTGCA GACAGAACTC TAAATAAGTT TGAGAGATTG 
TCACAGTCAT CAGGCACAGG AACAACAAAG ACTGCATTAG GCCCAAGAGT CCTACCAAAA 
CTACCTCAGA AAGTGGCACT TAGGAAAATA GACACCACTC ATCATCTCTC AATGGATAAG 
TATAACCAGC AACCCGAAAT CTTCCAGAAA TCTCCCCAGC CTGGAGATCT GCCTCAGAAG 
CCACAAATTG CAGATCTTCC TCCGAAACCG CAGCCTTTGG ATATCGCCCA GAAACCACAC 
GTCGGAGATC TACCTCCCAA GCCCGGAGAA TTGCCTCCTA AACCTCAGCT AGGAGATTTG 
CCCCCGAAAC CGCAAGATTT ACCTCCAAAG CCACAGCTGA AAGATCTTCC GCCCAAGCCC 
AACCTGGCAG AGGTGATGTG CAAAACTCCT GTTGCGGATA TAGTCCCCAA ACCTCATACC 
AATGAGGCCA ATCAAAATTC CCAGCCGGTG GATGCGACCC ATAATGCACA GCCCACGGAC 
CAAACCCCTG TCCAGAAGCA AGTTCCAGAA ACTTCCAATA ATGGCCCACC GCCTCTTCCA 
GAAACACCCG TGCCACTTCC CCGAAAGATA AACACAGGGA AGAATAAGCT TAAACGAGTT 
AAAACAATAT ACGACTGCCA AGCGGACAAT GATGATGAAC TCACATTTGC GGAGGGGGAA 
GTGATTATCG TCACTGGAGA GGAGGACCAG GAGTGGTGGA TTGGGCACAT CGAAGGCTTC 
CCGGACAGGA AAGGAGTATT CCCTGTGTCC TTCGTCCACC TGTTGACTGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819587.2
CDS	533..3886
Translation	MMPDQISVSEFISETTEDYNSPTTSSFTTRLQSCRNSINVLEEALDQDRTALQKVKKSVKAIYNSGQDHVQNEENYAQALDKFGSNFLSRDNADLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGVKGDLKKPFDRAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDQKESRRDSQSKQAGYSMHQLQGNKEYGSEKKGYLLKKSDGIRKVWQRRKCTVKNGILTISHATSNRPPTKLNLLTCQVKPNADEKKCFDLISHNRTYHFQAEDEQEYIAWISVLTNSKEDALNMAFRGDQSTGESSLEDLTKAIIDDVQKTPGNEVCCDCGSPDPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDKLGTSELLLAKNVGNNSFNDIMEGNLLSPSAKPSPSSDMIARKEYITAKYVERRFSRKICSTGAEKLNELLEAVKSRDLLALIQVYAEGVEIMEPLLESGQEPGETALHYAVRTADHTSLPLVDFLVQNCGNLDKQTGKGNTALHYCCLYNKPECLKLLLRGKPTIDAVNHIGETAFDVAKRQKAAQCEELVSQALAGKLNPHVHVEYDWNLRQEEMDESDDDLEDKPSPIKKERSPRPQSFCHSSSISPQDKLSLPGFSTPRDKQRLSYGAFTNQIFMSTSTDSPTSPTADAPPLPPRNAGKVASGPTSTLPLSTQSPSGSSTLSKKRPPPPPPGHKRTLSDPPSPLPHGPPSKGSVVCGNDLGPSAADRTLNKFERLSQSSGTGTTKTALGPRVLPKLPQKVALRKIDTTHHLSMDKYNQQPEIFQKSPQPGDLPQKPQIADLPPKPQPLDIAQKPHVGDLPPKPGELPPKPQLGDLPPKPQDLPPKPQLKDLPPKPNLAEVMCKTPVADIVPKPHTNEANQNSQPVDATHNAQPTDQTPVQKQVPETSNNGPPPLPETPVPLPRKINTGKNKLKRVKTIYDCQADNDDELTFAEGEVIIVTGEEDQEWWIGHIEGFPDRKGVFPVSFVHLLTE

Target ORF information:

RefSeq Version	XM_012964133.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (asap1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012964133.3

ORF Insert Sequence:

ATGATGCCCG ATCAGATCTC AGTATCGGAG TTTATATCGG AGACCACAGA GGATTACAAT 
TCGCCAACCA CGTCCAGTTT CACGACCCGT CTTCAGAGCT GCAGGAATTC GATCAATGTG 
CTGGAAGAGG CCTTAGATCA AGATAGGACA GCGTTGCAGA AAGTGAAAAA ATCGGTGAAA 
GCAATTTATA ATTCAGGCCA AGATCATGTC CAAAATGAAG AAAACTATGC ACAAGCCTTA 
GATAAATTTG GAAGTAATTT TCTAAGTCGG GATAACGCAG ATTTGGGCAC CGCCTTCGTG 
AAATTCTCCA CTCTAACAAA AGAACTATCA ACGCTTCTTA AAAACCTGCT ACAGGGATTA 
AGCCACAATG TCATCTTCAC CTTGGATTCT CTCTTGAAAG GGGACTTAAA GGGCGTAAAA 
GGGGACCTTA AGAAGCCATT CGACAGAGCG TGGAAGGATT ATGAAACCAA ATTCACGAAA 
ATCGAAAAGG AGAAAAGGGA ACACGCCAAG CAACATGGGA TGATCCGTAC GGAGATTACT 
GGGGCCGAGA TTGCCGAAGA GATGGAGAAG GAGAGGAGAT TATTCCAGCT ACAGATGTGT 
GAGTATCTCA TTAAAGTTAA TGAAATCAAG ACTAAGAAAG GCGTGGATCT GCTGCAGAAC 
CTTATAAAGT ACTATCACGC ACAGTGCAAT TTCTTCCAGG ATGGATTGAA AACAGCTGAC 
AAGCTGAAGC AGTACATAGA GAAATTGGCT GCAGATTTGT ACAATATTAA GCAGACCCAA 
GATGAGGAGA AGAAACAGCT CACTGCCCTG CGAGACCTGA TAAAATCCTC TTTACAGCTG 
GATCAGAAGG AGTCTAGAAG AGATTCCCAG AGTAAGCAGG CAGGCTACAG CATGCACCAG 
CTCCAGGGGA ACAAGGAGTA TGGCAGCGAG AAGAAAGGCT ACCTGCTGAA AAAAAGTGAT 
GGCATCCGGA AAGTATGGCA GAGGAGGAAG TGCACTGTGA AAAATGGAAT CCTGACCATT 
TCCCATGCAA CGTCAAACAG GCCACCGACA AAGCTGAACC TGCTGACCTG CCAAGTCAAG 
CCGAACGCTG ATGAGAAGAA ATGCTTTGAT CTCATCTCAC ACAACAGAAC ATACCACTTC 
CAAGCAGAGG ACGAACAAGA ATACATTGCG TGGATATCAG TTCTGACAAA TAGCAAAGAA 
GATGCACTCA ATATGGCTTT CCGGGGAGAC CAGAGCACTG GCGAGAGCAG CCTGGAGGAT 
CTGACTAAGG CCATTATAGA CGATGTACAG AAAACGCCTG GAAATGAAGT TTGCTGTGAT 
TGTGGGTCAC CGGACCCGAC ATGGCTTTCC ACAAACCTTG GGATTTTAAC CTGTATAGAA 
TGTTCGGGCA TACATAGGGA AATGGGGGTT CATATCTCTA GAATCCAATC TCTGGAATTA 
GACAAATTAG GGACTTCTGA ACTCTTGCTG GCCAAGAATG TAGGAAACAA TAGCTTCAAT 
GATATTATGG AAGGGAATTT GCTGTCTCCG TCAGCCAAGC CTTCTCCCTC CAGTGATATG 
ATTGCACGTA AAGAATATAT CACTGCCAAG TACGTGGAGC GGAGGTTTTC CAGAAAGATC 
TGTTCTACTG GGGCAGAAAA ACTGAATGAG CTACTTGAGG CTGTGAAATC CAGGGACTTA 
CTTGCACTAA TCCAAGTCTA TGCAGAAGGT GTAGAGATCA TGGAACCACT GCTAGAATCT 
GGACAGGAGC CAGGTGAAAC TGCACTTCAT TATGCAGTCC GGACAGCTGA CCACACGTCT 
CTTCCCTTGG TGGACTTCCT GGTACAAAAC TGTGGAAATT TAGATAAGCA GACTGGAAAG 
GGCAACACTG CTTTACACTA CTGCTGCTTA TATAACAAGC CGGAATGCTT AAAATTGCTT 
TTGAGGGGAA AGCCAACTAT TGATGCTGTT AACCATATTG GTGAGACGGC TTTTGATGTA 
GCAAAGAGAC AGAAAGCAGC CCAGTGTGAG GAACTGGTAT CCCAAGCCCT GGCAGGGAAG 
CTGAATCCTC ATGTTCACGT GGAGTACGAC TGGAACCTGC GTCAGGAAGA AATGGATGAG 
AGCGACGATG ATTTGGAGGA TAAGCCAAGC CCAATAAAGA AAGAGCGTTC CCCCAGACCC 
CAGAGCTTCT GCCATTCGTC CAGCATTTCC CCACAAGACA AGCTGTCGCT GCCAGGTTTC 
AGTACTCCTC GGGACAAGCA GAGACTCTCT TATGGCGCCT TTACCAACCA AATATTCATG 
TCTACAAGCA CAGATTCCCC CACGTCTCCT ACTGCAGATG CCCCTCCGCT GCCCCCTAGG 
AACGCAGGCA AAGTGGCGAG TGGCCCTACT TCAACCCTCC CTCTAAGCAC CCAATCCCCT 
AGTGGCAGTT CCACTCTGTC CAAGAAGAGA CCTCCTCCCC CACCCCCGGG ACACAAACGA 
ACCCTCTCCG ACCCACCAAG TCCGCTACCT CATGGCCCCC CCAGTAAGGG CTCAGTTGTT 
TGTGGTAATG ACTTGGGCCC GAGTGCTGCA GACAGAACTC TAAATAAGTT TGAGAGATTG 
TCACAGTCAT CAGGCACAGG AACAACAAAG ACTGCATTAG GCCCAAGAGT CCTACCAAAA 
CTACCTCAGA AAGTGGCACT TAGGAAAATA GACACCACTC ATCATCTCTC AATGGATAAG 
TATAACCAGC AACCCGAAAT CTTCCAGAAA TCTCCCCAGC CTGGAGATCT GCCTCAGAAG 
CCACAAATTG CAGATCTTCC TCCGAAACCG CAGCCTTTGG ATATCGCCCA GAAACCACAC 
GTCGGAGATC TACCTCCCAA GCCCGGAGAA TTGCCTCCTA AACCTCAGCT AGGAGATTTG 
CCCCCGAAAC CGCAAGATTT ACCTCCAAAG CCACAGCTGA AAGATCTTCC GCCCAAGCCC 
AACCTGGCAG AGGTGATGTG CAAAACTCCT GTTGCGGATA TAGTCCCCAA ACCTCATACC 
AATGAGGCCA ATCAAAATTC CCAGCCGGTG GATGCGACCC ATAATGCACA GCCCACGGAC 
CAAACCCCTG TCCAGAAGCA AGTTCCAGAA ACTTCCAATA ATGGCCCACC GCCTCTTCCA 
GAAACACCCG TGCCACTTCC CCGAAAGATA AACACAGGGA AGAATAAGCT TAAACGAGTT 
AAAACAATAT ACGACTGCCA AGCGGACAAT GATGATGAAC TCACATTTGC GGAGGGGGAA 
GTGATTATCG TCACTGGAGA GGAGGACCAG GAGTGGTGGA TTGGGCACAT CGAAGGCTTC 
CCGGACAGGA AAGGAGTATT CCCTGTGTCC TTCGTCCACC TGTTGACTGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

asap1 ( XM_012964134.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819588.2 Xenopus tropicalis ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (asap1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa59454
Clone ID Related Accession (Same CDS sequence)	XM_012964134.3
Accession Version	XM_012964134.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012964134.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGATC AGATCTCAGT ATCGGAGTTT ATATCGGAGA CCACAGAGGA TTACAATTCG 
CCAACCACGT CCAGTTTCAC GACCCGTCTT CAGAGCTGCA GGAATTCGAT CAATGTGCTG 
GAAGAGGCCT TAGATCAAGA TAGGACAGCG TTGCAGAAAG TGAAAAAATC GGTGAAAGCA 
ATTTATAATT CAGGCCAAGA TCATGTCCAA AATGAAGAAA ACTATGCACA AGCCTTAGAT 
AAATTTGGAA GTAATTTTCT AAGTCGGGAT AACGCAGATT TGGGCACCGC CTTCGTGAAA 
TTCTCCACTC TAACAAAAGA ACTATCAACG CTTCTTAAAA ACCTGCTACA GGGATTAAGC 
CACAATGTCA TCTTCACCTT GGATTCTCTC TTGAAAGGGG ACTTAAAGGG CGTAAAAGGG 
GACCTTAAGA AGCCATTCGA CAGAGCGTGG AAGGATTATG AAACCAAATT CACGAAAATC 
GAAAAGGAGA AAAGGGAACA CGCCAAGCAA CATGGGATGA TCCGTACGGA GATTACTGGG 
GCCGAGATTG CCGAAGAGAT GGAGAAGGAG AGGAGATTAT TCCAGCTACA GATGTGTGAG 
TATCTCATTA AAGTTAATGA AATCAAGACT AAGAAAGGCG TGGATCTGCT GCAGAACCTT 
ATAAAGTACT ATCACGCACA GTGCAATTTC TTCCAGGATG GATTGAAAAC AGCTGACAAG 
CTGAAGCAGT ACATAGAGAA ATTGGCTGCA GATTTGTACA ATATTAAGCA GACCCAAGAT 
GAGGAGAAGA AACAGCTCAC TGCCCTGCGA GACCTGATAA AATCCTCTTT ACAGCTGGAT 
CAGAAGGAGT CTAGAAGAGA TTCCCAGAGT AAGCAGGCAG GCTACAGCAT GCACCAGCTC 
CAGGGGAACA AGGAGTATGG CAGCGAGAAG AAAGGCTACC TGCTGAAAAA AAGTGATGGC 
ATCCGGAAAG TATGGCAGAG GAGGAAGTGC ACTGTGAAAA ATGGAATCCT GACCATTTCC 
CATGCAACGT CAAACAGGCC ACCGACAAAG CTGAACCTGC TGACCTGCCA AGTCAAGCCG 
AACGCTGATG AGAAGAAATG CTTTGATCTC ATCTCACACA ACAGAACATA CCACTTCCAA 
GCAGAGGACG AACAAGAATA CATTGCGTGG ATATCAGTTC TGACAAATAG CAAAGAAGAT 
GCACTCAATA TGGCTTTCCG GGGAGACCAG AGCACTGGCG AGAGCAGCCT GGAGGATCTG 
ACTAAGGCCA TTATAGACGA TGTACAGAAA ACGCCTGGAA ATGAAGTTTG CTGTGATTGT 
GGGTCACCGG ACCCGACATG GCTTTCCACA AACCTTGGGA TTTTAACCTG TATAGAATGT 
TCGGGCATAC ATAGGGAAAT GGGGGTTCAT ATCTCTAGAA TCCAATCTCT GGAATTAGAC 
AAATTAGGGA CTTCTGAACT CTTGCTGGCC AAGAATGTAG GAAACAATAG CTTCAATGAT 
ATTATGGAAG GGAATTTGCT GTCTCCGTCA GCCAAGCCTT CTCCCTCCAG TGATATGATT 
GCACGTAAAG AATATATCAC TGCCAAGTAC GTGGAGCGGA GGTTTTCCAG AAAGATCTGT 
TCTACTGGGG CAGAAAAACT GAATGAGCTA CTTGAGGCTG TGAAATCCAG GGACTTACTT 
GCACTAATCC AAGTCTATGC AGAAGGTGTA GAGATCATGG AACCACTGCT AGAATCTGGA 
CAGGAGCCAG GTGAAACTGC ACTTCATTAT GCAGTCCGGA CAGCTGACCA CACGTCTCTT 
CCCTTGGTGG ACTTCCTGGT ACAAAACTGT GGAAATTTAG ATAAGCAGAC TGGAAAGGGC 
AACACTGCTT TACACTACTG CTGCTTATAT AACAAGCCGG AATGCTTAAA ATTGCTTTTG 
AGGGGAAAGC CAACTATTGA TGCTGTTAAC CATATTGGTG AGACGGCTTT TGATGTAGCA 
AAGAGACAGA AAGCAGCCCA GTGTGAGGAA CTGGTATCCC AAGCCCTGGC AGGGAAGCTG 
AATCCTCATG TTCACGTGGA GTACGACTGG AACCTGCGTC AGGAAGAAAT GGATGAGAGC 
GACGATGATT TGGAGGATAA GCCAAGCCCA ATAAAGAAAG AGCGTTCCCC CAGACCCCAG 
AGCTTCTGCC ATTCGTCCAG CATTTCCCCA CAAGACAAGC TGTCGCTGCC AGGTTTCAGT 
ACTCCTCGGG ACAAGCAGAG ACTCTCTTAT GGCGCCTTTA CCAACCAAAT ATTCATGTCT 
ACAAGCACAG ATTCCCCCAC GTCTCCTACT GCAGATGCCC CTCCGCTGCC CCCTAGGAAC 
GCAGGCAAAG TGGCGAGTGG CCCTACTTCA ACCCTCCCTC TAAGCACCCA ATCCCCTAGT 
GGCAGTTCCA CTCTGTCCAA GAAGAGACCT CCTCCCCCAC CCCCGGGACA CAAACGAACC 
CTCTCCGACC CACCAAGTCC GCTACCTCAT GGCCCCCCCA GTAAGGGCTC AGTTGTTTGT 
GGTAATGACT TGGGCCCGAG TGCTGCAGAC AGAACTCTAA ATAAGTTTGA GAGATTGTCA 
CAGTCATCAG GCACAGGAAC AACAAAGACT GCATTAGGCC CAAGAGTCCT ACCAAAACTA 
CCTCAGAAAG TGGCACTTAG GAAAATAGAC ACCACTCATC ATCTCTCAAT GGATAAGTAT 
AACCAGCAAC CCGAAATCTT CCAGAAATCT CCCCAGCCTG GAGATCTGCC TCAGAAGCCA 
CAAATTGCAG ATCTTCCTCC GAAACCGCAG CCTTTGGATA TCGCCCAGAA ACCACACGTC 
GGAGATCTAC CTCCCAAGCC CGGAGAATTG CCTCCTAAAC CTCAGCTAGG AGATTTGCCC 
CCGAAACCGC AAGATTTACC TCCAAAGCCA CAGCTGAAAG ATCTTCCGCC CAAGCCCAAC 
CTGGCAGAGG TGATGTGCAA AACTCCTGTT GCGGATATAG TCCCCAAACC TCATACCAAT 
GAGGCCAATC AAAATTCCCA GCCGGTGGAT GCGACCCATA ATGCACAGCC CACGGACCAA 
ACCCCTGTCC AGAAGCAAGT TCCAGAAACT TCCAATAATG GCCCACCGCC TCTTCCAGAA 
ACACCCGTGC CACTTCCCCG AAAGATAAAC ACAGGGAAGA ATAAGCTTAA ACGAGTTAAA 
ACAATATACG ACTGCCAAGC GGACAATGAT GATGAACTCA CATTTGCGGA GGGGGAAGTG 
ATTATCGTCA CTGGAGAGGA GGACCAGGAG TGGTGGATTG GGCACATCGA AGGCTTCCCG 
GACAGGAAAG GAGTATTCCC TGTGTCCTTC GTCCACCTGT TGACTGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819588.2
CDS	340..3690
Translation	MPDQISVSEFISETTEDYNSPTTSSFTTRLQSCRNSINVLEEALDQDRTALQKVKKSVKAIYNSGQDHVQNEENYAQALDKFGSNFLSRDNADLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGVKGDLKKPFDRAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDQKESRRDSQSKQAGYSMHQLQGNKEYGSEKKGYLLKKSDGIRKVWQRRKCTVKNGILTISHATSNRPPTKLNLLTCQVKPNADEKKCFDLISHNRTYHFQAEDEQEYIAWISVLTNSKEDALNMAFRGDQSTGESSLEDLTKAIIDDVQKTPGNEVCCDCGSPDPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDKLGTSELLLAKNVGNNSFNDIMEGNLLSPSAKPSPSSDMIARKEYITAKYVERRFSRKICSTGAEKLNELLEAVKSRDLLALIQVYAEGVEIMEPLLESGQEPGETALHYAVRTADHTSLPLVDFLVQNCGNLDKQTGKGNTALHYCCLYNKPECLKLLLRGKPTIDAVNHIGETAFDVAKRQKAAQCEELVSQALAGKLNPHVHVEYDWNLRQEEMDESDDDLEDKPSPIKKERSPRPQSFCHSSSISPQDKLSLPGFSTPRDKQRLSYGAFTNQIFMSTSTDSPTSPTADAPPLPPRNAGKVASGPTSTLPLSTQSPSGSSTLSKKRPPPPPPGHKRTLSDPPSPLPHGPPSKGSVVCGNDLGPSAADRTLNKFERLSQSSGTGTTKTALGPRVLPKLPQKVALRKIDTTHHLSMDKYNQQPEIFQKSPQPGDLPQKPQIADLPPKPQPLDIAQKPHVGDLPPKPGELPPKPQLGDLPPKPQDLPPKPQLKDLPPKPNLAEVMCKTPVADIVPKPHTNEANQNSQPVDATHNAQPTDQTPVQKQVPETSNNGPPPLPETPVPLPRKINTGKNKLKRVKTIYDCQADNDDELTFAEGEVIIVTGEEDQEWWIGHIEGFPDRKGVFPVSFVHLLTE

Target ORF information:

RefSeq Version	XM_012964134.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (asap1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012964134.3

ORF Insert Sequence:

ATGCCCGATC AGATCTCAGT ATCGGAGTTT ATATCGGAGA CCACAGAGGA TTACAATTCG 
CCAACCACGT CCAGTTTCAC GACCCGTCTT CAGAGCTGCA GGAATTCGAT CAATGTGCTG 
GAAGAGGCCT TAGATCAAGA TAGGACAGCG TTGCAGAAAG TGAAAAAATC GGTGAAAGCA 
ATTTATAATT CAGGCCAAGA TCATGTCCAA AATGAAGAAA ACTATGCACA AGCCTTAGAT 
AAATTTGGAA GTAATTTTCT AAGTCGGGAT AACGCAGATT TGGGCACCGC CTTCGTGAAA 
TTCTCCACTC TAACAAAAGA ACTATCAACG CTTCTTAAAA ACCTGCTACA GGGATTAAGC 
CACAATGTCA TCTTCACCTT GGATTCTCTC TTGAAAGGGG ACTTAAAGGG CGTAAAAGGG 
GACCTTAAGA AGCCATTCGA CAGAGCGTGG AAGGATTATG AAACCAAATT CACGAAAATC 
GAAAAGGAGA AAAGGGAACA CGCCAAGCAA CATGGGATGA TCCGTACGGA GATTACTGGG 
GCCGAGATTG CCGAAGAGAT GGAGAAGGAG AGGAGATTAT TCCAGCTACA GATGTGTGAG 
TATCTCATTA AAGTTAATGA AATCAAGACT AAGAAAGGCG TGGATCTGCT GCAGAACCTT 
ATAAAGTACT ATCACGCACA GTGCAATTTC TTCCAGGATG GATTGAAAAC AGCTGACAAG 
CTGAAGCAGT ACATAGAGAA ATTGGCTGCA GATTTGTACA ATATTAAGCA GACCCAAGAT 
GAGGAGAAGA AACAGCTCAC TGCCCTGCGA GACCTGATAA AATCCTCTTT ACAGCTGGAT 
CAGAAGGAGT CTAGAAGAGA TTCCCAGAGT AAGCAGGCAG GCTACAGCAT GCACCAGCTC 
CAGGGGAACA AGGAGTATGG CAGCGAGAAG AAAGGCTACC TGCTGAAAAA AAGTGATGGC 
ATCCGGAAAG TATGGCAGAG GAGGAAGTGC ACTGTGAAAA ATGGAATCCT GACCATTTCC 
CATGCAACGT CAAACAGGCC ACCGACAAAG CTGAACCTGC TGACCTGCCA AGTCAAGCCG 
AACGCTGATG AGAAGAAATG CTTTGATCTC ATCTCACACA ACAGAACATA CCACTTCCAA 
GCAGAGGACG AACAAGAATA CATTGCGTGG ATATCAGTTC TGACAAATAG CAAAGAAGAT 
GCACTCAATA TGGCTTTCCG GGGAGACCAG AGCACTGGCG AGAGCAGCCT GGAGGATCTG 
ACTAAGGCCA TTATAGACGA TGTACAGAAA ACGCCTGGAA ATGAAGTTTG CTGTGATTGT 
GGGTCACCGG ACCCGACATG GCTTTCCACA AACCTTGGGA TTTTAACCTG TATAGAATGT 
TCGGGCATAC ATAGGGAAAT GGGGGTTCAT ATCTCTAGAA TCCAATCTCT GGAATTAGAC 
AAATTAGGGA CTTCTGAACT CTTGCTGGCC AAGAATGTAG GAAACAATAG CTTCAATGAT 
ATTATGGAAG GGAATTTGCT GTCTCCGTCA GCCAAGCCTT CTCCCTCCAG TGATATGATT 
GCACGTAAAG AATATATCAC TGCCAAGTAC GTGGAGCGGA GGTTTTCCAG AAAGATCTGT 
TCTACTGGGG CAGAAAAACT GAATGAGCTA CTTGAGGCTG TGAAATCCAG GGACTTACTT 
GCACTAATCC AAGTCTATGC AGAAGGTGTA GAGATCATGG AACCACTGCT AGAATCTGGA 
CAGGAGCCAG GTGAAACTGC ACTTCATTAT GCAGTCCGGA CAGCTGACCA CACGTCTCTT 
CCCTTGGTGG ACTTCCTGGT ACAAAACTGT GGAAATTTAG ATAAGCAGAC TGGAAAGGGC 
AACACTGCTT TACACTACTG CTGCTTATAT AACAAGCCGG AATGCTTAAA ATTGCTTTTG 
AGGGGAAAGC CAACTATTGA TGCTGTTAAC CATATTGGTG AGACGGCTTT TGATGTAGCA 
AAGAGACAGA AAGCAGCCCA GTGTGAGGAA CTGGTATCCC AAGCCCTGGC AGGGAAGCTG 
AATCCTCATG TTCACGTGGA GTACGACTGG AACCTGCGTC AGGAAGAAAT GGATGAGAGC 
GACGATGATT TGGAGGATAA GCCAAGCCCA ATAAAGAAAG AGCGTTCCCC CAGACCCCAG 
AGCTTCTGCC ATTCGTCCAG CATTTCCCCA CAAGACAAGC TGTCGCTGCC AGGTTTCAGT 
ACTCCTCGGG ACAAGCAGAG ACTCTCTTAT GGCGCCTTTA CCAACCAAAT ATTCATGTCT 
ACAAGCACAG ATTCCCCCAC GTCTCCTACT GCAGATGCCC CTCCGCTGCC CCCTAGGAAC 
GCAGGCAAAG TGGCGAGTGG CCCTACTTCA ACCCTCCCTC TAAGCACCCA ATCCCCTAGT 
GGCAGTTCCA CTCTGTCCAA GAAGAGACCT CCTCCCCCAC CCCCGGGACA CAAACGAACC 
CTCTCCGACC CACCAAGTCC GCTACCTCAT GGCCCCCCCA GTAAGGGCTC AGTTGTTTGT 
GGTAATGACT TGGGCCCGAG TGCTGCAGAC AGAACTCTAA ATAAGTTTGA GAGATTGTCA 
CAGTCATCAG GCACAGGAAC AACAAAGACT GCATTAGGCC CAAGAGTCCT ACCAAAACTA 
CCTCAGAAAG TGGCACTTAG GAAAATAGAC ACCACTCATC ATCTCTCAAT GGATAAGTAT 
AACCAGCAAC CCGAAATCTT CCAGAAATCT CCCCAGCCTG GAGATCTGCC TCAGAAGCCA 
CAAATTGCAG ATCTTCCTCC GAAACCGCAG CCTTTGGATA TCGCCCAGAA ACCACACGTC 
GGAGATCTAC CTCCCAAGCC CGGAGAATTG CCTCCTAAAC CTCAGCTAGG AGATTTGCCC 
CCGAAACCGC AAGATTTACC TCCAAAGCCA CAGCTGAAAG ATCTTCCGCC CAAGCCCAAC 
CTGGCAGAGG TGATGTGCAA AACTCCTGTT GCGGATATAG TCCCCAAACC TCATACCAAT 
GAGGCCAATC AAAATTCCCA GCCGGTGGAT GCGACCCATA ATGCACAGCC CACGGACCAA 
ACCCCTGTCC AGAAGCAAGT TCCAGAAACT TCCAATAATG GCCCACCGCC TCTTCCAGAA 
ACACCCGTGC CACTTCCCCG AAAGATAAAC ACAGGGAAGA ATAAGCTTAA ACGAGTTAAA 
ACAATATACG ACTGCCAAGC GGACAATGAT GATGAACTCA CATTTGCGGA GGGGGAAGTG 
ATTATCGTCA CTGGAGAGGA GGACCAGGAG TGGTGGATTG GGCACATCGA AGGCTTCCCG 
GACAGGAAAG GAGTATTCCC TGTGTCCTTC GTCCACCTGT TGACTGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

asap1 ( XM_031903144.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031759004.1 Xenopus tropicalis ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (asap1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXa59455
Clone ID Related Accession (Same CDS sequence)	XM_031903144.1
Accession Version	XM_031903144.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3240bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGATCTT CGGCCTCTAG ACTTTCGAGT TTTTCCTCTA GAGACTCACT ATGGAACCGG 
ATGCCCGATC AGATCTCAGT ATCGGAGTTT ATATCGGAGA CCACAGAGGA TTACAATTCG 
CCAACCACGT CCAGTTTCAC GACCCGTCTT CAGAGCTGCA GGAATTCGAT CAATGTGCTG 
GAAGAGGCCT TAGATCAAGA TAGGACAGCG TTGCAGAAAG TGAAAAAATC GGTGAAAGCA 
ATTTATAATT CAGGCCAAGA TCATGTCCAA AATGAAGAAA ACTATGCACA AGCCTTAGAT 
AAATTTGGAA GTAATTTTCT AAGTCGGGAT AACGCAGATT TGGGCACCGC CTTCGTGAAA 
TTCTCCACTC TAACAAAAGA ACTATCAACG CTTCTTAAAA ACCTGCTACA GGGATTAAGC 
CACAATGTCA TCTTCACCTT GGATTCTCTC TTGAAAGGGG ACTTAAAGGG CGTAAAAGGG 
GACCTTAAGA AGCCATTCGA CAGAGCGTGG AAGGATTATG AAACCAAATT CACGAAAATC 
GAAAAGGAGA AAAGGGAACA CGCCAAGCAA CATGGGATGA TCCGTACGGA GATTACTGGG 
GCCGAGATTG CCGAAGAGAT GGAGAAGGAG AGGAGATTAT TCCAGCTACA GATGTGTGAG 
TATCTCATTA AAGTTAATGA AATCAAGACT AAGAAAGGCG TGGATCTGCT GCAGAACCTT 
ATAAAGTACT ATCACGCACA GTGCAATTTC TTCCAGGATG GATTGAAAAC AGCTGACAAG 
CTGAAGCAGT ACATAGAGAA ATTGGCTGCA GATTTGTACA ATATTAAGCA GACCCAAGAT 
GAGGAGAAGA AACAGCTCAC TGCCCTGCGA GACCTGATAA AATCCTCTTT ACAGCTGGAT 
CAGAAGGAGT CTAGAAGAGA TTCCCAGAGT AAGCAGGCAG GCTACAGCAT GCACCAGCTC 
CAGGGGAACA AGGAGTATGG CAGCGAGAAG AAAGGCTACC TGCTGAAAAA AAGTGATGGC 
ATCCGGAAAG TATGGCAGAG GAGGAAGTGC ACTGTGAAAA ATGGAATCCT GACCATTTCC 
CATGCAACGT CAAACAGGCC ACCGACAAAG CTGAACCTGC TGACCTGCCA AGTCAAGCCG 
AACGCTGATG AGAAGAAATG CTTTGATCTC ATCTCACACA ACAGAACATA CCACTTCCAA 
GCAGAGGACG AACAAGAATA CATTGCGTGG ATATCAGTTC TGACAAATAG CAAAGAAGAT 
GCACTCAATA TGGCTTTCCG GGGAGACCAG AGCACTGGCG AGAGCAGCCT GGAGGATCTG 
ACTAAGGCCA TTATAGACGA TGTACAGAAA ACGCCTGGAA ATGAAGTTTG CTGTGATTGT 
GGGTCACCGG ACCCGACATG GCTTTCCACA AACCTTGGGA TTTTAACCTG TATAGAATGT 
TCGGGCATAC ATAGGGAAAT GGGGGTTCAT ATCTCTAGAA TCCAATCTCT GGAATTAGAC 
AAATTAGGGA CTTCTGAACT CTTGCTGGCC AAGAATGTAG GAAACAATAG CTTCAATGAT 
ATTATGGAAG GGAATTTGCT GTCTCCGTCA GCCAAGCCTT CTCCCTCCAG TGATATGATT 
GCACGTAAAG AATATATCAC TGCCAAGTAC GTGGAGCGGA GGTTTTCCAG AAAGATCTGT 
TCTACTGGGG CAGAAAAACT GAATGAGCTA CTTGAGGCTG TGAAATCCAG GGACTTACTT 
GCACTAATCC AAGTCTATGC AGAAGGTGTA GAGATCATGG AACCACTGCT AGAATCTGGA 
CAGGAGCCAG GTGAAACTGC ACTTCATTAT GCAGTCCGGA CAGCTGACCA CACGTCTCTT 
CCCTTGGTGG ACTTCCTGGT ACAAAACTGT GGAAATTTAG ATAAGCAGAC TGGAAAGGGC 
AACACTGCTT TACACTACTG CTGCTTATAT AACAAGCCGG AATGCTTAAA ATTGCTTTTG 
AGGGGAAAGC CAACTATTGA TGCTGTTAAC CATATTGGTG AGACGGCTTT TGATGTAGCA 
AAGAGACAGA AAGCAGCCCA GTGTGAGGAA CTGGTATCCC AAGCCCTGGC AGGGAAGCTG 
AATCCTCATG TTCACGTGGA GTACGACTGG AACCTGCGTC AGGAAGAAAT GGATGAGAGC 
GACGATGATT TGGAGGATAA GCCAAGCCCA ATAAAGAAAG AGCGTTCCCC CAGACCCCAG 
AGCTTCTGCC ATTCGTCCAG CATTTCCCCA CAAGACAAGC TGTCGCTGCC AGGTTTCAGT 
ACTCCTCGGG ACAAGCAGAG ACTCTCTTAT GGCGCCTTTA CCAACCAAAT ATTCATGTCT 
ACAAGCACAG ATTCCCCCAC GTCTCCTACT GCAGATGCCC CTCCGCTGCC CCCTAGGAAC 
GCAGGCAAAG GTAATGACTT GGGCCCGAGT GCTGCAGACA GAACTCTAAA TAAGTTTGAG 
AGATTGTCAC AGTCATCAGG CACAGGAACA ACAAAGACTG CATTAGGCCC AAGAGTCCTA 
CCAAAACTAC CTCAGAAAGT GGCACTTAGG AAAATAGACA CCACTCATCA TCTCTCAATG 
GATAAGTATA ACCAGCAACC CGAAATCTTC CAGAAATCTC CCCAGCCTGG AGATCTGCCT 
CAGAAGCCAC AAATTGCAGA TCTTCCTCCG AAACCGCAGC CTTTGGATAT CGCCCAGAAA 
CCACACGTCG GAGATCTACC TCCCAAGCCC GGAGAATTGC CTCCTAAACC TCAGCTAGGA 
GATTTGCCCC CGAAACCGCA AGATTTACCT CCAAAGCCAC AGCTGAAAGA TCTTCCGCCC 
AAGCCCAACC TGGCAGAGGT GATGTGCAAA ACTCCTGTTG CGGATATAGT CCCCAAACCT 
CATACCAATG AGGCCAATCA AAATTCCCAG CCGGTGGATG CGACCCATAA TGCACAGCCC 
ACGGACCAAA CCCCTGTCCA GAAGCAAGTT CCAGAAACTT CCAATAATGG CCCACCGCCT 
CTTCCAGAAA CACCCGTGCC ACTTCCCCGA AAGATAAACA CAGGGAAGAA TAAGCTTAAA 
CGAGTTAAAA CAATATACGA CTGCCAAGCG GACAATGATG ATGAACTCAC ATTTGCGGAG 
GGGGAAGTGA TTATCGTCAC TGGAGAGGAG GACCAGGAGT GGTGGATTGG GCACATCGAA 
GGCTTCCCGG ACAGGAAAGG AGTATTCCCT GTGTCCTTCG TCCACCTGTT GACTGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031759004.1
CDS	313..3552
Translation	MRSSASRLSSFSSRDSLWNRMPDQISVSEFISETTEDYNSPTTSSFTTRLQSCRNSINVLEEALDQDRTALQKVKKSVKAIYNSGQDHVQNEENYAQALDKFGSNFLSRDNADLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGVKGDLKKPFDRAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDQKESRRDSQSKQAGYSMHQLQGNKEYGSEKKGYLLKKSDGIRKVWQRRKCTVKNGILTISHATSNRPPTKLNLLTCQVKPNADEKKCFDLISHNRTYHFQAEDEQEYIAWISVLTNSKEDALNMAFRGDQSTGESSLEDLTKAIIDDVQKTPGNEVCCDCGSPDPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDKLGTSELLLAKNVGNNSFNDIMEGNLLSPSAKPSPSSDMIARKEYITAKYVERRFSRKICSTGAEKLNELLEAVKSRDLLALIQVYAEGVEIMEPLLESGQEPGETALHYAVRTADHTSLPLVDFLVQNCGNLDKQTGKGNTALHYCCLYNKPECLKLLLRGKPTIDAVNHIGETAFDVAKRQKAAQCEELVSQALAGKLNPHVHVEYDWNLRQEEMDESDDDLEDKPSPIKKERSPRPQSFCHSSSISPQDKLSLPGFSTPRDKQRLSYGAFTNQIFMSTSTDSPTSPTADAPPLPPRNAGKGNDLGPSAADRTLNKFERLSQSSGTGTTKTALGPRVLPKLPQKVALRKIDTTHHLSMDKYNQQPEIFQKSPQPGDLPQKPQIADLPPKPQPLDIAQKPHVGDLPPKPGELPPKPQLGDLPPKPQDLPPKPQLKDLPPKPNLAEVMCKTPVADIVPKPHTNEANQNSQPVDATHNAQPTDQTPVQKQVPETSNNGPPPLPETPVPLPRKINTGKNKLKRVKTIYDCQADNDDELTFAEGEVIIVTGEEDQEWWIGHIEGFPDRKGVFPVSFVHLLTE

Target ORF information:

RefSeq Version	XM_031903144.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (asap1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031903144.1

ORF Insert Sequence:

ATGCGATCTT CGGCCTCTAG ACTTTCGAGT TTTTCCTCTA GAGACTCACT ATGGAACCGG 
ATGCCCGATC AGATCTCAGT ATCGGAGTTT ATATCGGAGA CCACAGAGGA TTACAATTCG 
CCAACCACGT CCAGTTTCAC GACCCGTCTT CAGAGCTGCA GGAATTCGAT CAATGTGCTG 
GAAGAGGCCT TAGATCAAGA TAGGACAGCG TTGCAGAAAG TGAAAAAATC GGTGAAAGCA 
ATTTATAATT CAGGCCAAGA TCATGTCCAA AATGAAGAAA ACTATGCACA AGCCTTAGAT 
AAATTTGGAA GTAATTTTCT AAGTCGGGAT AACGCAGATT TGGGCACCGC CTTCGTGAAA 
TTCTCCACTC TAACAAAAGA ACTATCAACG CTTCTTAAAA ACCTGCTACA GGGATTAAGC 
CACAATGTCA TCTTCACCTT GGATTCTCTC TTGAAAGGGG ACTTAAAGGG CGTAAAAGGG 
GACCTTAAGA AGCCATTCGA CAGAGCGTGG AAGGATTATG AAACCAAATT CACGAAAATC 
GAAAAGGAGA AAAGGGAACA CGCCAAGCAA CATGGGATGA TCCGTACGGA GATTACTGGG 
GCCGAGATTG CCGAAGAGAT GGAGAAGGAG AGGAGATTAT TCCAGCTACA GATGTGTGAG 
TATCTCATTA AAGTTAATGA AATCAAGACT AAGAAAGGCG TGGATCTGCT GCAGAACCTT 
ATAAAGTACT ATCACGCACA GTGCAATTTC TTCCAGGATG GATTGAAAAC AGCTGACAAG 
CTGAAGCAGT ACATAGAGAA ATTGGCTGCA GATTTGTACA ATATTAAGCA GACCCAAGAT 
GAGGAGAAGA AACAGCTCAC TGCCCTGCGA GACCTGATAA AATCCTCTTT ACAGCTGGAT 
CAGAAGGAGT CTAGAAGAGA TTCCCAGAGT AAGCAGGCAG GCTACAGCAT GCACCAGCTC 
CAGGGGAACA AGGAGTATGG CAGCGAGAAG AAAGGCTACC TGCTGAAAAA AAGTGATGGC 
ATCCGGAAAG TATGGCAGAG GAGGAAGTGC ACTGTGAAAA ATGGAATCCT GACCATTTCC 
CATGCAACGT CAAACAGGCC ACCGACAAAG CTGAACCTGC TGACCTGCCA AGTCAAGCCG 
AACGCTGATG AGAAGAAATG CTTTGATCTC ATCTCACACA ACAGAACATA CCACTTCCAA 
GCAGAGGACG AACAAGAATA CATTGCGTGG ATATCAGTTC TGACAAATAG CAAAGAAGAT 
GCACTCAATA TGGCTTTCCG GGGAGACCAG AGCACTGGCG AGAGCAGCCT GGAGGATCTG 
ACTAAGGCCA TTATAGACGA TGTACAGAAA ACGCCTGGAA ATGAAGTTTG CTGTGATTGT 
GGGTCACCGG ACCCGACATG GCTTTCCACA AACCTTGGGA TTTTAACCTG TATAGAATGT 
TCGGGCATAC ATAGGGAAAT GGGGGTTCAT ATCTCTAGAA TCCAATCTCT GGAATTAGAC 
AAATTAGGGA CTTCTGAACT CTTGCTGGCC AAGAATGTAG GAAACAATAG CTTCAATGAT 
ATTATGGAAG GGAATTTGCT GTCTCCGTCA GCCAAGCCTT CTCCCTCCAG TGATATGATT 
GCACGTAAAG AATATATCAC TGCCAAGTAC GTGGAGCGGA GGTTTTCCAG AAAGATCTGT 
TCTACTGGGG CAGAAAAACT GAATGAGCTA CTTGAGGCTG TGAAATCCAG GGACTTACTT 
GCACTAATCC AAGTCTATGC AGAAGGTGTA GAGATCATGG AACCACTGCT AGAATCTGGA 
CAGGAGCCAG GTGAAACTGC ACTTCATTAT GCAGTCCGGA CAGCTGACCA CACGTCTCTT 
CCCTTGGTGG ACTTCCTGGT ACAAAACTGT GGAAATTTAG ATAAGCAGAC TGGAAAGGGC 
AACACTGCTT TACACTACTG CTGCTTATAT AACAAGCCGG AATGCTTAAA ATTGCTTTTG 
AGGGGAAAGC CAACTATTGA TGCTGTTAAC CATATTGGTG AGACGGCTTT TGATGTAGCA 
AAGAGACAGA AAGCAGCCCA GTGTGAGGAA CTGGTATCCC AAGCCCTGGC AGGGAAGCTG 
AATCCTCATG TTCACGTGGA GTACGACTGG AACCTGCGTC AGGAAGAAAT GGATGAGAGC 
GACGATGATT TGGAGGATAA GCCAAGCCCA ATAAAGAAAG AGCGTTCCCC CAGACCCCAG 
AGCTTCTGCC ATTCGTCCAG CATTTCCCCA CAAGACAAGC TGTCGCTGCC AGGTTTCAGT 
ACTCCTCGGG ACAAGCAGAG ACTCTCTTAT GGCGCCTTTA CCAACCAAAT ATTCATGTCT 
ACAAGCACAG ATTCCCCCAC GTCTCCTACT GCAGATGCCC CTCCGCTGCC CCCTAGGAAC 
GCAGGCAAAG GTAATGACTT GGGCCCGAGT GCTGCAGACA GAACTCTAAA TAAGTTTGAG 
AGATTGTCAC AGTCATCAGG CACAGGAACA ACAAAGACTG CATTAGGCCC AAGAGTCCTA 
CCAAAACTAC CTCAGAAAGT GGCACTTAGG AAAATAGACA CCACTCATCA TCTCTCAATG 
GATAAGTATA ACCAGCAACC CGAAATCTTC CAGAAATCTC CCCAGCCTGG AGATCTGCCT 
CAGAAGCCAC AAATTGCAGA TCTTCCTCCG AAACCGCAGC CTTTGGATAT CGCCCAGAAA 
CCACACGTCG GAGATCTACC TCCCAAGCCC GGAGAATTGC CTCCTAAACC TCAGCTAGGA 
GATTTGCCCC CGAAACCGCA AGATTTACCT CCAAAGCCAC AGCTGAAAGA TCTTCCGCCC 
AAGCCCAACC TGGCAGAGGT GATGTGCAAA ACTCCTGTTG CGGATATAGT CCCCAAACCT 
CATACCAATG AGGCCAATCA AAATTCCCAG CCGGTGGATG CGACCCATAA TGCACAGCCC 
ACGGACCAAA CCCCTGTCCA GAAGCAAGTT CCAGAAACTT CCAATAATGG CCCACCGCCT 
CTTCCAGAAA CACCCGTGCC ACTTCCCCGA AAGATAAACA CAGGGAAGAA TAAGCTTAAA 
CGAGTTAAAA CAATATACGA CTGCCAAGCG GACAATGATG ATGAACTCAC ATTTGCGGAG 
GGGGAAGTGA TTATCGTCAC TGGAGAGGAG GACCAGGAGT GGTGGATTGG GCACATCGAA 
GGCTTCCCGG ACAGGAAAGG AGTATTCCCT GTGTCCTTCG TCCACCTGTT GACTGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

asap1 ( XM_031903145.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031759005.1 Xenopus tropicalis ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (asap1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OXa59456
Clone ID Related Accession (Same CDS sequence)	XM_031903145.1
Accession Version	XM_031903145.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGATCTT CGGCCTCTAG ACTTTCGAGT TTTTCCTCTA GAGACTCACT ATGGAACCGG 
ATGCCCGATC AGATCTCAGT ATCGGAGTTT ATATCGGAGA CCACAGAGGA TTACAATTCG 
CCAACCACGT CCAGTTTCAC GACCCGTCTT CAGAGCTGCA GGAATTCGAT CAATGTGCTG 
GAAGAGGCCT TAGATCAAGA TAGGACAGCG TTGCAGAAAG TGAAAAAATC GGTGAAAGCA 
ATTTATAATT CAGGCCAAGA TCATGTCCAA AATGAAGAAA ACTATGCACA AGCCTTAGAT 
AAATTTGGAA GTAATTTTCT AAGTCGGGAT AACGCAGATT TGGGCACCGC CTTCGTGAAA 
TTCTCCACTC TAACAAAAGA ACTATCAACG CTTCTTAAAA ACCTGCTACA GGGATTAAGC 
CACAATGTCA TCTTCACCTT GGATTCTCTC TTGAAAGGGG ACTTAAAGGG CGTAAAAGGG 
GACCTTAAGA AGCCATTCGA CAGAGCGTGG AAGGATTATG AAACCAAATT CACGAAAATC 
GAAAAGGAGA AAAGGGAACA CGCCAAGCAA CATGGGATGA TCCGTACGGA GATTACTGGG 
GCCGAGATTG CCGAAGAGAT GGAGAAGGAG AGGAGATTAT TCCAGCTACA GATGTGTGAG 
TATCTCATTA AAGTTAATGA AATCAAGACT AAGAAAGGCG TGGATCTGCT GCAGAACCTT 
ATAAAGTACT ATCACGCACA GTGCAATTTC TTCCAGGATG GATTGAAAAC AGCTGACAAG 
CTGAAGCAGT ACATAGAGAA ATTGGCTGCA GATTTGTACA ATATTAAGCA GACCCAAGAT 
GAGGAGAAGA AACAGCTCAC TGCCCTGCGA GACCTGATAA AATCCTCTTT ACAGCTGGAT 
CAGAAGGAGG ATTCCCAGAG TAAGCAGGCA GGCTACAGCA TGCACCAGCT CCAGGGGAAC 
AAGGAGTATG GCAGCGAGAA GAAAGGCTAC CTGCTGAAAA AAAGTGATGG CATCCGGAAA 
GTATGGCAGA GGAGGAAGTG CACTGTGAAA AATGGAATCC TGACCATTTC CCATGCAACG 
TCAAACAGGC CACCGACAAA GCTGAACCTG CTGACCTGCC AAGTCAAGCC GAACGCTGAT 
GAGAAGAAAT GCTTTGATCT CATCTCACAC AACAGAACAT ACCACTTCCA AGCAGAGGAC 
GAACAAGAAT ACATTGCGTG GATATCAGTT CTGACAAATA GCAAAGAAGA TGCACTCAAT 
ATGGCTTTCC GGGGAGACCA GAGCACTGGC GAGAGCAGCC TGGAGGATCT GACTAAGGCC 
ATTATAGACG ATGTACAGAA AACGCCTGGA AATGAAGTTT GCTGTGATTG TGGGTCACCG 
GACCCGACAT GGCTTTCCAC AAACCTTGGG ATTTTAACCT GTATAGAATG TTCGGGCATA 
CATAGGGAAA TGGGGGTTCA TATCTCTAGA ATCCAATCTC TGGAATTAGA CAAATTAGGG 
ACTTCTGAAC TCTTGCTGGC CAAGAATGTA GGAAACAATA GCTTCAATGA TATTATGGAA 
GGGAATTTGC TGTCTCCGTC AGCCAAGCCT TCTCCCTCCA GTGATATGAT TGCACGTAAA 
GAATATATCA CTGCCAAGTA CGTGGAGCGG AGGTTTTCCA GAAAGATCTG TTCTACTGGG 
GCAGAAAAAC TGAATGAGCT ACTTGAGGCT GTGAAATCCA GGGACTTACT TGCACTAATC 
CAAGTCTATG CAGAAGGTGT AGAGATCATG GAACCACTGC TAGAATCTGG ACAGGAGCCA 
GGTGAAACTG CACTTCATTA TGCAGTCCGG ACAGCTGACC ACACGTCTCT TCCCTTGGTG 
GACTTCCTGG TACAAAACTG TGGAAATTTA GATAAGCAGA CTGGAAAGGG CAACACTGCT 
TTACACTACT GCTGCTTATA TAACAAGCCG GAATGCTTAA AATTGCTTTT GAGGGGAAAG 
CCAACTATTG ATGCTGTTAA CCATATTGGT GAGACGGCTT TTGATGTAGC AAAGAGACAG 
AAAGCAGCCC AGTGTGAGGA ACTGGTATCC CAAGCCCTGG CAGGGAAGCT GAATCCTCAT 
GTTCACGTGG AGTACGACTG GAACCTGCGT CAGGAAGAAA TGGATGAGAG CGACGATGAT 
TTGGAGGATA AGCCAAGCCC AATAAAGAAA GAGCGTTCCC CCAGACCCCA GAGCTTCTGC 
CATTCGTCCA GCATTTCCCC ACAAGACAAG CTGTCGCTGC CAGGTTTCAG TACTCCTCGG 
GACAAGCAGA GACTCTCTTA TGGCGCCTTT ACCAACCAAA TATTCATGTC TACAAGCACA 
GATTCCCCCA CGTCTCCTAC TGCAGATGCC CCTCCGCTGC CCCCTAGGAA CGCAGGCAAA 
GGTAATGACT TGGGCCCGAG TGCTGCAGAC AGAACTCTAA ATAAGTTTGA GAGATTGTCA 
CAGTCATCAG GCACAGGAAC AACAAAGACT GCATTAGGCC CAAGAGTCCT ACCAAAACTA 
CCTCAGAAAG TGGCACTTAG GAAAATAGAC ACCACTCATC ATCTCTCAAT GGATAAGTAT 
AACCAGCAAC CCGAAATCTT CCAGAAATCT CCCCAGCCTG GAGATCTGCC TCAGAAGCCA 
CAAATTGCAG ATCTTCCTCC GAAACCGCAG CCTTTGGATA TCGCCCAGAA ACCACACGTC 
GGAGATCTAC CTCCCAAGCC CGGAGAATTG CCTCCTAAAC CTCAGCTAGG AGATTTGCCC 
CCGAAACCGC AAGATTTACC TCCAAAGCCA CAGCTGAAAG ATCTTCCGCC CAAGCCCAAC 
CTGGCAGAGG TGATGTGCAA AACTCCTGTT GCGGATATAG TCCCCAAACC TCATACCAAT 
GAGGCCAATC AAAATTCCCA GCCGGTGGAT GCGACCCATA ATGCACAGCC CACGGACCAA 
ACCCCTGTCC AGAAGCAAGT TCCAGAAACT TCCAATAATG GCCCACCGCC TCTTCCAGAA 
ACACCCGTGC CACTTCCCCG AAAGATAAAC ACAGGGAAGA ATAAGCTTAA ACGAGTTAAA 
ACAATATACG ACTGCCAAGC GGACAATGAT GATGAACTCA CATTTGCGGA GGGGGAAGTG 
ATTATCGTCA CTGGAGAGGA GGACCAGGAG TGGTGGATTG GGCACATCGA AGGCTTCCCG 
GACAGGAAAG GAGTATTCCC TGTGTCCTTC GTCCACCTGT TGACTGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031759005.1
CDS	312..3542
Translation	MRSSASRLSSFSSRDSLWNRMPDQISVSEFISETTEDYNSPTTSSFTTRLQSCRNSINVLEEALDQDRTALQKVKKSVKAIYNSGQDHVQNEENYAQALDKFGSNFLSRDNADLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGVKGDLKKPFDRAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDQKEDSQSKQAGYSMHQLQGNKEYGSEKKGYLLKKSDGIRKVWQRRKCTVKNGILTISHATSNRPPTKLNLLTCQVKPNADEKKCFDLISHNRTYHFQAEDEQEYIAWISVLTNSKEDALNMAFRGDQSTGESSLEDLTKAIIDDVQKTPGNEVCCDCGSPDPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDKLGTSELLLAKNVGNNSFNDIMEGNLLSPSAKPSPSSDMIARKEYITAKYVERRFSRKICSTGAEKLNELLEAVKSRDLLALIQVYAEGVEIMEPLLESGQEPGETALHYAVRTADHTSLPLVDFLVQNCGNLDKQTGKGNTALHYCCLYNKPECLKLLLRGKPTIDAVNHIGETAFDVAKRQKAAQCEELVSQALAGKLNPHVHVEYDWNLRQEEMDESDDDLEDKPSPIKKERSPRPQSFCHSSSISPQDKLSLPGFSTPRDKQRLSYGAFTNQIFMSTSTDSPTSPTADAPPLPPRNAGKGNDLGPSAADRTLNKFERLSQSSGTGTTKTALGPRVLPKLPQKVALRKIDTTHHLSMDKYNQQPEIFQKSPQPGDLPQKPQIADLPPKPQPLDIAQKPHVGDLPPKPGELPPKPQLGDLPPKPQDLPPKPQLKDLPPKPNLAEVMCKTPVADIVPKPHTNEANQNSQPVDATHNAQPTDQTPVQKQVPETSNNGPPPLPETPVPLPRKINTGKNKLKRVKTIYDCQADNDDELTFAEGEVIIVTGEEDQEWWIGHIEGFPDRKGVFPVSFVHLLTE

Target ORF information:

RefSeq Version	XM_031903145.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (asap1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031903145.1

ORF Insert Sequence:

ATGCGATCTT CGGCCTCTAG ACTTTCGAGT TTTTCCTCTA GAGACTCACT ATGGAACCGG 
ATGCCCGATC AGATCTCAGT ATCGGAGTTT ATATCGGAGA CCACAGAGGA TTACAATTCG 
CCAACCACGT CCAGTTTCAC GACCCGTCTT CAGAGCTGCA GGAATTCGAT CAATGTGCTG 
GAAGAGGCCT TAGATCAAGA TAGGACAGCG TTGCAGAAAG TGAAAAAATC GGTGAAAGCA 
ATTTATAATT CAGGCCAAGA TCATGTCCAA AATGAAGAAA ACTATGCACA AGCCTTAGAT 
AAATTTGGAA GTAATTTTCT AAGTCGGGAT AACGCAGATT TGGGCACCGC CTTCGTGAAA 
TTCTCCACTC TAACAAAAGA ACTATCAACG CTTCTTAAAA ACCTGCTACA GGGATTAAGC 
CACAATGTCA TCTTCACCTT GGATTCTCTC TTGAAAGGGG ACTTAAAGGG CGTAAAAGGG 
GACCTTAAGA AGCCATTCGA CAGAGCGTGG AAGGATTATG AAACCAAATT CACGAAAATC 
GAAAAGGAGA AAAGGGAACA CGCCAAGCAA CATGGGATGA TCCGTACGGA GATTACTGGG 
GCCGAGATTG CCGAAGAGAT GGAGAAGGAG AGGAGATTAT TCCAGCTACA GATGTGTGAG 
TATCTCATTA AAGTTAATGA AATCAAGACT AAGAAAGGCG TGGATCTGCT GCAGAACCTT 
ATAAAGTACT ATCACGCACA GTGCAATTTC TTCCAGGATG GATTGAAAAC AGCTGACAAG 
CTGAAGCAGT ACATAGAGAA ATTGGCTGCA GATTTGTACA ATATTAAGCA GACCCAAGAT 
GAGGAGAAGA AACAGCTCAC TGCCCTGCGA GACCTGATAA AATCCTCTTT ACAGCTGGAT 
CAGAAGGAGG ATTCCCAGAG TAAGCAGGCA GGCTACAGCA TGCACCAGCT CCAGGGGAAC 
AAGGAGTATG GCAGCGAGAA GAAAGGCTAC CTGCTGAAAA AAAGTGATGG CATCCGGAAA 
GTATGGCAGA GGAGGAAGTG CACTGTGAAA AATGGAATCC TGACCATTTC CCATGCAACG 
TCAAACAGGC CACCGACAAA GCTGAACCTG CTGACCTGCC AAGTCAAGCC GAACGCTGAT 
GAGAAGAAAT GCTTTGATCT CATCTCACAC AACAGAACAT ACCACTTCCA AGCAGAGGAC 
GAACAAGAAT ACATTGCGTG GATATCAGTT CTGACAAATA GCAAAGAAGA TGCACTCAAT 
ATGGCTTTCC GGGGAGACCA GAGCACTGGC GAGAGCAGCC TGGAGGATCT GACTAAGGCC 
ATTATAGACG ATGTACAGAA AACGCCTGGA AATGAAGTTT GCTGTGATTG TGGGTCACCG 
GACCCGACAT GGCTTTCCAC AAACCTTGGG ATTTTAACCT GTATAGAATG TTCGGGCATA 
CATAGGGAAA TGGGGGTTCA TATCTCTAGA ATCCAATCTC TGGAATTAGA CAAATTAGGG 
ACTTCTGAAC TCTTGCTGGC CAAGAATGTA GGAAACAATA GCTTCAATGA TATTATGGAA 
GGGAATTTGC TGTCTCCGTC AGCCAAGCCT TCTCCCTCCA GTGATATGAT TGCACGTAAA 
GAATATATCA CTGCCAAGTA CGTGGAGCGG AGGTTTTCCA GAAAGATCTG TTCTACTGGG 
GCAGAAAAAC TGAATGAGCT ACTTGAGGCT GTGAAATCCA GGGACTTACT TGCACTAATC 
CAAGTCTATG CAGAAGGTGT AGAGATCATG GAACCACTGC TAGAATCTGG ACAGGAGCCA 
GGTGAAACTG CACTTCATTA TGCAGTCCGG ACAGCTGACC ACACGTCTCT TCCCTTGGTG 
GACTTCCTGG TACAAAACTG TGGAAATTTA GATAAGCAGA CTGGAAAGGG CAACACTGCT 
TTACACTACT GCTGCTTATA TAACAAGCCG GAATGCTTAA AATTGCTTTT GAGGGGAAAG 
CCAACTATTG ATGCTGTTAA CCATATTGGT GAGACGGCTT TTGATGTAGC AAAGAGACAG 
AAAGCAGCCC AGTGTGAGGA ACTGGTATCC CAAGCCCTGG CAGGGAAGCT GAATCCTCAT 
GTTCACGTGG AGTACGACTG GAACCTGCGT CAGGAAGAAA TGGATGAGAG CGACGATGAT 
TTGGAGGATA AGCCAAGCCC AATAAAGAAA GAGCGTTCCC CCAGACCCCA GAGCTTCTGC 
CATTCGTCCA GCATTTCCCC ACAAGACAAG CTGTCGCTGC CAGGTTTCAG TACTCCTCGG 
GACAAGCAGA GACTCTCTTA TGGCGCCTTT ACCAACCAAA TATTCATGTC TACAAGCACA 
GATTCCCCCA CGTCTCCTAC TGCAGATGCC CCTCCGCTGC CCCCTAGGAA CGCAGGCAAA 
GGTAATGACT TGGGCCCGAG TGCTGCAGAC AGAACTCTAA ATAAGTTTGA GAGATTGTCA 
CAGTCATCAG GCACAGGAAC AACAAAGACT GCATTAGGCC CAAGAGTCCT ACCAAAACTA 
CCTCAGAAAG TGGCACTTAG GAAAATAGAC ACCACTCATC ATCTCTCAAT GGATAAGTAT 
AACCAGCAAC CCGAAATCTT CCAGAAATCT CCCCAGCCTG GAGATCTGCC TCAGAAGCCA 
CAAATTGCAG ATCTTCCTCC GAAACCGCAG CCTTTGGATA TCGCCCAGAA ACCACACGTC 
GGAGATCTAC CTCCCAAGCC CGGAGAATTG CCTCCTAAAC CTCAGCTAGG AGATTTGCCC 
CCGAAACCGC AAGATTTACC TCCAAAGCCA CAGCTGAAAG ATCTTCCGCC CAAGCCCAAC 
CTGGCAGAGG TGATGTGCAA AACTCCTGTT GCGGATATAG TCCCCAAACC TCATACCAAT 
GAGGCCAATC AAAATTCCCA GCCGGTGGAT GCGACCCATA ATGCACAGCC CACGGACCAA 
ACCCCTGTCC AGAAGCAAGT TCCAGAAACT TCCAATAATG GCCCACCGCC TCTTCCAGAA 
ACACCCGTGC CACTTCCCCG AAAGATAAAC ACAGGGAAGA ATAAGCTTAA ACGAGTTAAA 
ACAATATACG ACTGCCAAGC GGACAATGAT GATGAACTCA CATTTGCGGA GGGGGAAGTG 
ATTATCGTCA CTGGAGAGGA GGACCAGGAG TGGTGGATTG GGCACATCGA AGGCTTCCCG 
GACAGGAAAG GAGTATTCCC TGTGTCCTTC GTCCACCTGT TGACTGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

asap1 ( XM_012964138.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819592.2 Xenopus tropicalis ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (asap1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OXa59457
Clone ID Related Accession (Same CDS sequence)	XM_012964138.3
Accession Version	XM_012964138.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3174bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012964138.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCCCG ATCAGATCTC AGTATCGGAG TTTATATCGG AGACCACAGA GGATTACAAT 
TCGCCAACCA CGTCCAGTTT CACGACCCGT CTTCAGAGCT GCAGGAATTC GATCAATGTG 
CTGGAAGAGG CCTTAGATCA AGATAGGACA GCGTTGCAGA AAGTGAAAAA ATCGGTGAAA 
GCAATTTATA ATTCAGGCCA AGATCATGTC CAAAATGAAG AAAACTATGC ACAAGCCTTA 
GATAAATTTG GAAGTAATTT TCTAAGTCGG GATAACGCAG ATTTGGGCAC CGCCTTCGTG 
AAATTCTCCA CTCTAACAAA AGAACTATCA ACGCTTCTTA AAAACCTGCT ACAGGGATTA 
AGCCACAATG TCATCTTCAC CTTGGATTCT CTCTTGAAAG GGGACTTAAA GGGCGTAAAA 
GGGGACCTTA AGAAGCCATT CGACAGAGCG TGGAAGGATT ATGAAACCAA ATTCACGAAA 
ATCGAAAAGG AGAAAAGGGA ACACGCCAAG CAACATGGGA TGATCCGTAC GGAGATTACT 
GGGGCCGAGA TTGCCGAAGA GATGGAGAAG GAGAGGAGAT TATTCCAGCT ACAGATGTGT 
GAGTATCTCA TTAAAGTTAA TGAAATCAAG ACTAAGAAAG GCGTGGATCT GCTGCAGAAC 
CTTATAAAGT ACTATCACGC ACAGTGCAAT TTCTTCCAGG ATGGATTGAA AACAGCTGAC 
AAGCTGAAGC AGTACATAGA GAAATTGGCT GCAGATTTGT ACAATATTAA GCAGACCCAA 
GATGAGGAGA AGAAACAGCT CACTGCCCTG CGAGACCTGA TAAAATCCTC TTTACAGCTG 
GATCAGAAGG AGGATTCCCA GAGTAAGCAG GCAGGCTACA GCATGCACCA GCTCCAGGGG 
AACAAGGAGT ATGGCAGCGA GAAGAAAGGC TACCTGCTGA AAAAAAGTGA TGGCATCCGG 
AAAGTATGGC AGAGGAGGAA GTGCACTGTG AAAAATGGAA TCCTGACCAT TTCCCATGCA 
ACGTCAAACA GGCCACCGAC AAAGCTGAAC CTGCTGACCT GCCAAGTCAA GCCGAACGCT 
GATGAGAAGA AATGCTTTGA TCTCATCTCA CACAACAGAA CATACCACTT CCAAGCAGAG 
GACGAACAAG AATACATTGC GTGGATATCA GTTCTGACAA ATAGCAAAGA AGATGCACTC 
AATATGGCTT TCCGGGGAGA CCAGAGCACT GGCGAGAGCA GCCTGGAGGA TCTGACTAAG 
GCCATTATAG ACGATGTACA GAAAACGCCT GGAAATGAAG TTTGCTGTGA TTGTGGGTCA 
CCGGACCCGA CATGGCTTTC CACAAACCTT GGGATTTTAA CCTGTATAGA ATGTTCGGGC 
ATACATAGGG AAATGGGGGT TCATATCTCT AGAATCCAAT CTCTGGAATT AGACAAATTA 
GGGACTTCTG AACTCTTGCT GGCCAAGAAT GTAGGAAACA ATAGCTTCAA TGATATTATG 
GAAGGGAATT TGCTGTCTCC GTCAGCCAAG CCTTCTCCCT CCAGTGATAT GATTGCACGT 
AAAGAATATA TCACTGCCAA GTACGTGGAG CGGAGGTTTT CCAGAAAGAT CTGTTCTACT 
GGGGCAGAAA AACTGAATGA GCTACTTGAG GCTGTGAAAT CCAGGGACTT ACTTGCACTA 
ATCCAAGTCT ATGCAGAAGG TGTAGAGATC ATGGAACCAC TGCTAGAATC TGGACAGGAG 
CCAGGTGAAA CTGCACTTCA TTATGCAGTC CGGACAGCTG ACCACACGTC TCTTCCCTTG 
GTGGACTTCC TGGTACAAAA CTGTGGAAAT TTAGATAAGC AGACTGGAAA GGGCAACACT 
GCTTTACACT ACTGCTGCTT ATATAACAAG CCGGAATGCT TAAAATTGCT TTTGAGGGGA 
AAGCCAACTA TTGATGCTGT TAACCATATT GGTGAGACGG CTTTTGATGT AGCAAAGAGA 
CAGAAAGCAG CCCAGTGTGA GGAACTGGTA TCCCAAGCCC TGGCAGGGAA GCTGAATCCT 
CATGTTCACG TGGAGTACGA CTGGAACCTG CGTCAGGAAG AAATGGATGA GAGCGACGAT 
GATTTGGAGG ATAAGCCAAG CCCAATAAAG AAAGAGCGTT CCCCCAGACC CCAGAGCTTC 
TGCCATTCGT CCAGCATTTC CCCACAAGAC AAGCTGTCGC TGCCAGGTTT CAGTACTCCT 
CGGGACAAGC AGAGACTCTC TTATGGCGCC TTTACCAACC AAATATTCAT GTCTACAAGC 
ACAGATTCCC CCACGTCTCC TACTGCAGAT GCCCCTCCGC TGCCCCCTAG GAACGCAGGC 
AAAGGTAATG ACTTGGGCCC GAGTGCTGCA GACAGAACTC TAAATAAGTT TGAGAGATTG 
TCACAGTCAT CAGGCACAGG AACAACAAAG ACTGCATTAG GCCCAAGAGT CCTACCAAAA 
CTACCTCAGA AAGTGGCACT TAGGAAAATA GACACCACTC ATCATCTCTC AATGGATAAG 
TATAACCAGC AACCCGAAAT CTTCCAGAAA TCTCCCCAGC CTGGAGATCT GCCTCAGAAG 
CCACAAATTG CAGATCTTCC TCCGAAACCG CAGCCTTTGG ATATCGCCCA GAAACCACAC 
GTCGGAGATC TACCTCCCAA GCCCGGAGAA TTGCCTCCTA AACCTCAGCT AGGAGATTTG 
CCCCCGAAAC CGCAAGATTT ACCTCCAAAG CCACAGCTGA AAGATCTTCC GCCCAAGCCC 
AACCTGGCAG AGGTGATGTG CAAAACTCCT GTTGCGGATA TAGTCCCCAA ACCTCATACC 
AATGAGGCCA ATCAAAATTC CCAGCCGGTG GATGCGACCC ATAATGCACA GCCCACGGAC 
CAAACCCCTG TCCAGAAGCA AGTTCCAGAA ACTTCCAATA ATGGCCCACC GCCTCTTCCA 
GAAACACCCG TGCCACTTCC CCGAAAGATA AACACAGGGA AGAATAAGCT TAAACGAGTT 
AAAACAATAT ACGACTGCCA AGCGGACAAT GATGATGAAC TCACATTTGC GGAGGGGGAA 
GTGATTATCG TCACTGGAGA GGAGGACCAG GAGTGGTGGA TTGGGCACAT CGAAGGCTTC 
CCGGACAGGA AAGGAGTATT CCCTGTGTCC TTCGTCCACC TGTTGACTGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819592.2
CDS	532..3705
Translation	MMPDQISVSEFISETTEDYNSPTTSSFTTRLQSCRNSINVLEEALDQDRTALQKVKKSVKAIYNSGQDHVQNEENYAQALDKFGSNFLSRDNADLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGVKGDLKKPFDRAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDQKEDSQSKQAGYSMHQLQGNKEYGSEKKGYLLKKSDGIRKVWQRRKCTVKNGILTISHATSNRPPTKLNLLTCQVKPNADEKKCFDLISHNRTYHFQAEDEQEYIAWISVLTNSKEDALNMAFRGDQSTGESSLEDLTKAIIDDVQKTPGNEVCCDCGSPDPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDKLGTSELLLAKNVGNNSFNDIMEGNLLSPSAKPSPSSDMIARKEYITAKYVERRFSRKICSTGAEKLNELLEAVKSRDLLALIQVYAEGVEIMEPLLESGQEPGETALHYAVRTADHTSLPLVDFLVQNCGNLDKQTGKGNTALHYCCLYNKPECLKLLLRGKPTIDAVNHIGETAFDVAKRQKAAQCEELVSQALAGKLNPHVHVEYDWNLRQEEMDESDDDLEDKPSPIKKERSPRPQSFCHSSSISPQDKLSLPGFSTPRDKQRLSYGAFTNQIFMSTSTDSPTSPTADAPPLPPRNAGKGNDLGPSAADRTLNKFERLSQSSGTGTTKTALGPRVLPKLPQKVALRKIDTTHHLSMDKYNQQPEIFQKSPQPGDLPQKPQIADLPPKPQPLDIAQKPHVGDLPPKPGELPPKPQLGDLPPKPQDLPPKPQLKDLPPKPNLAEVMCKTPVADIVPKPHTNEANQNSQPVDATHNAQPTDQTPVQKQVPETSNNGPPPLPETPVPLPRKINTGKNKLKRVKTIYDCQADNDDELTFAEGEVIIVTGEEDQEWWIGHIEGFPDRKGVFPVSFVHLLTE

Target ORF information:

RefSeq Version	XM_012964138.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (asap1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012964138.3

ORF Insert Sequence:

ATGATGCCCG ATCAGATCTC AGTATCGGAG TTTATATCGG AGACCACAGA GGATTACAAT 
TCGCCAACCA CGTCCAGTTT CACGACCCGT CTTCAGAGCT GCAGGAATTC GATCAATGTG 
CTGGAAGAGG CCTTAGATCA AGATAGGACA GCGTTGCAGA AAGTGAAAAA ATCGGTGAAA 
GCAATTTATA ATTCAGGCCA AGATCATGTC CAAAATGAAG AAAACTATGC ACAAGCCTTA 
GATAAATTTG GAAGTAATTT TCTAAGTCGG GATAACGCAG ATTTGGGCAC CGCCTTCGTG 
AAATTCTCCA CTCTAACAAA AGAACTATCA ACGCTTCTTA AAAACCTGCT ACAGGGATTA 
AGCCACAATG TCATCTTCAC CTTGGATTCT CTCTTGAAAG GGGACTTAAA GGGCGTAAAA 
GGGGACCTTA AGAAGCCATT CGACAGAGCG TGGAAGGATT ATGAAACCAA ATTCACGAAA 
ATCGAAAAGG AGAAAAGGGA ACACGCCAAG CAACATGGGA TGATCCGTAC GGAGATTACT 
GGGGCCGAGA TTGCCGAAGA GATGGAGAAG GAGAGGAGAT TATTCCAGCT ACAGATGTGT 
GAGTATCTCA TTAAAGTTAA TGAAATCAAG ACTAAGAAAG GCGTGGATCT GCTGCAGAAC 
CTTATAAAGT ACTATCACGC ACAGTGCAAT TTCTTCCAGG ATGGATTGAA AACAGCTGAC 
AAGCTGAAGC AGTACATAGA GAAATTGGCT GCAGATTTGT ACAATATTAA GCAGACCCAA 
GATGAGGAGA AGAAACAGCT CACTGCCCTG CGAGACCTGA TAAAATCCTC TTTACAGCTG 
GATCAGAAGG AGGATTCCCA GAGTAAGCAG GCAGGCTACA GCATGCACCA GCTCCAGGGG 
AACAAGGAGT ATGGCAGCGA GAAGAAAGGC TACCTGCTGA AAAAAAGTGA TGGCATCCGG 
AAAGTATGGC AGAGGAGGAA GTGCACTGTG AAAAATGGAA TCCTGACCAT TTCCCATGCA 
ACGTCAAACA GGCCACCGAC AAAGCTGAAC CTGCTGACCT GCCAAGTCAA GCCGAACGCT 
GATGAGAAGA AATGCTTTGA TCTCATCTCA CACAACAGAA CATACCACTT CCAAGCAGAG 
GACGAACAAG AATACATTGC GTGGATATCA GTTCTGACAA ATAGCAAAGA AGATGCACTC 
AATATGGCTT TCCGGGGAGA CCAGAGCACT GGCGAGAGCA GCCTGGAGGA TCTGACTAAG 
GCCATTATAG ACGATGTACA GAAAACGCCT GGAAATGAAG TTTGCTGTGA TTGTGGGTCA 
CCGGACCCGA CATGGCTTTC CACAAACCTT GGGATTTTAA CCTGTATAGA ATGTTCGGGC 
ATACATAGGG AAATGGGGGT TCATATCTCT AGAATCCAAT CTCTGGAATT AGACAAATTA 
GGGACTTCTG AACTCTTGCT GGCCAAGAAT GTAGGAAACA ATAGCTTCAA TGATATTATG 
GAAGGGAATT TGCTGTCTCC GTCAGCCAAG CCTTCTCCCT CCAGTGATAT GATTGCACGT 
AAAGAATATA TCACTGCCAA GTACGTGGAG CGGAGGTTTT CCAGAAAGAT CTGTTCTACT 
GGGGCAGAAA AACTGAATGA GCTACTTGAG GCTGTGAAAT CCAGGGACTT ACTTGCACTA 
ATCCAAGTCT ATGCAGAAGG TGTAGAGATC ATGGAACCAC TGCTAGAATC TGGACAGGAG 
CCAGGTGAAA CTGCACTTCA TTATGCAGTC CGGACAGCTG ACCACACGTC TCTTCCCTTG 
GTGGACTTCC TGGTACAAAA CTGTGGAAAT TTAGATAAGC AGACTGGAAA GGGCAACACT 
GCTTTACACT ACTGCTGCTT ATATAACAAG CCGGAATGCT TAAAATTGCT TTTGAGGGGA 
AAGCCAACTA TTGATGCTGT TAACCATATT GGTGAGACGG CTTTTGATGT AGCAAAGAGA 
CAGAAAGCAG CCCAGTGTGA GGAACTGGTA TCCCAAGCCC TGGCAGGGAA GCTGAATCCT 
CATGTTCACG TGGAGTACGA CTGGAACCTG CGTCAGGAAG AAATGGATGA GAGCGACGAT 
GATTTGGAGG ATAAGCCAAG CCCAATAAAG AAAGAGCGTT CCCCCAGACC CCAGAGCTTC 
TGCCATTCGT CCAGCATTTC CCCACAAGAC AAGCTGTCGC TGCCAGGTTT CAGTACTCCT 
CGGGACAAGC AGAGACTCTC TTATGGCGCC TTTACCAACC AAATATTCAT GTCTACAAGC 
ACAGATTCCC CCACGTCTCC TACTGCAGAT GCCCCTCCGC TGCCCCCTAG GAACGCAGGC 
AAAGGTAATG ACTTGGGCCC GAGTGCTGCA GACAGAACTC TAAATAAGTT TGAGAGATTG 
TCACAGTCAT CAGGCACAGG AACAACAAAG ACTGCATTAG GCCCAAGAGT CCTACCAAAA 
CTACCTCAGA AAGTGGCACT TAGGAAAATA GACACCACTC ATCATCTCTC AATGGATAAG 
TATAACCAGC AACCCGAAAT CTTCCAGAAA TCTCCCCAGC CTGGAGATCT GCCTCAGAAG 
CCACAAATTG CAGATCTTCC TCCGAAACCG CAGCCTTTGG ATATCGCCCA GAAACCACAC 
GTCGGAGATC TACCTCCCAA GCCCGGAGAA TTGCCTCCTA AACCTCAGCT AGGAGATTTG 
CCCCCGAAAC CGCAAGATTT ACCTCCAAAG CCACAGCTGA AAGATCTTCC GCCCAAGCCC 
AACCTGGCAG AGGTGATGTG CAAAACTCCT GTTGCGGATA TAGTCCCCAA ACCTCATACC 
AATGAGGCCA ATCAAAATTC CCAGCCGGTG GATGCGACCC ATAATGCACA GCCCACGGAC 
CAAACCCCTG TCCAGAAGCA AGTTCCAGAA ACTTCCAATA ATGGCCCACC GCCTCTTCCA 
GAAACACCCG TGCCACTTCC CCGAAAGATA AACACAGGGA AGAATAAGCT TAAACGAGTT 
AAAACAATAT ACGACTGCCA AGCGGACAAT GATGATGAAC TCACATTTGC GGAGGGGGAA 
GTGATTATCG TCACTGGAGA GGAGGACCAG GAGTGGTGGA TTGGGCACAT CGAAGGCTTC 
CCGGACAGGA AAGGAGTATT CCCTGTGTCC TTCGTCCACC TGTTGACTGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

asap1 ( NM_001097266.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001090735.1 Xenopus tropicalis ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (asap1), mRNA.

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CloneID	OXa23751
Clone ID Related Accession (Same CDS sequence)	NM_001097266.1
Accession Version	NM_001097266.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3171bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC129009.1. ##Evidence-Data-START## Transcript exon combination :: BC129009.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

ATGCCCGATC AGATCTCAGT ATCGGAGTTT ATATCGGAGA CCACAGAGGA TTACAATTCG 
CCAACCACAT CCAGTTTCAC GACCCGTCTT CAGAGCTGCA GGAATTCGAT CAATGTGCTG 
GAAGAGGCCT TAGATCAAGA TAGGACAGCG TTGCAGAAAG TGAAAAAATC GGTGAAAGCA 
ATTTATAATT CAGGCCAAGA TCATGTCCAA AATGAAGAAA ACTATGCACA AGCCTTAGAT 
AAATTTGGAA GTAATTTTCT AAGTCGGGAT AACGCAGATT TGGGCACCGC CTTCGTGAAA 
TTCTCCACTC TAACAAAAGA ACTATCAACG CTTCTTAAAA ACCTGCTACA GGGATTAAGC 
CACAATGTCA TCTTCACCTT GGATTCTCTC TTGAAAGGGG ACTTAAAGGG CGTAAAAGGG 
GACCTTAAGA AGCCATTCGA CAGAGCGTGG AAGGATTATG AAACCAAATT CACGAAAATC 
GAAAAGGAGA AAAGGGAACA CGCCAAGCAA CATGGGATGA TCCGTACGGA GATTACTGGG 
GCCGAGATTG CCGAAGAGAT GGAGAAGGAG AGGAGATTAT TCCAGCTACA GATGTGTGAG 
TATCTCATTA AAGTTAATGA AATCAAGACT AAGAAAGGCG TGGATCTGCT GCAGAACCTT 
ATAAAGTACT ATCACGCACA GTGCAATTTC TTCCAGGATG GATTGAAAAC AGCTGACAAG 
CTGAAGCAGT ACATAGAGAA ATTGGCTGCA GATTTGTACA ATATTAAGCA GACCCAAGAT 
GAGGAGAAGA AACAGCTCAC TGCCCTGCGA GACCTGATAA AATCCTCTTT ACAGCTGGAT 
CAGAAGGAGG ATTCCCAGAG TAAGCAGGCA GGCTACAGCA TGCACCAGCT CCAGGGGAAC 
AAGGAGTATG GCAGCGAGAA GAAAGGCTAC CTGCTGAAAA AAAGTGATGG CATCCGGAAA 
GTATGGCAGA GGAGGAAGTG CACTGTGAAA AATGGAATCC TGACCATTTC CCATGCAACG 
TCAAACAGGC CACCGACGAA GCTGAACCTG CTGACCTGCC AAGTCAAGCC GAACGCTGAT 
GAGAAGAAAT GCTTTGATCT CATCTCACAC AACAGAACAT ACCACTTCCA AGCAGAGGAC 
GAACAAGAAT ACATTGCGTG GATATCAGTT CTGACAAATA GCAAAGAAGA TGCACTCAAT 
ATGGCTTTCC GGGGAGACCA GAGCACTGGC GAGAGCAGCC TGGAGGATCT GACTAAGGCC 
ATTATAGACG ATGTACAGAA AACGCCTGGA AATGAAGTTT GCTGTGATTG TGGGTCACCG 
GACCCGACAT GGCTTTCCAC AAACCTTGGG ATTTTAACCT GTATAGAATG TTCGGGCATA 
CATAGGGAAA TGGGGGTTCA TATCTCTAGA ATCCAATCTC TGGAATTAGA CAAATTAGGG 
ACTTCTGAAC TCTTGCTGGC CAAGAATGTA GGAAACAATA GCTTCAATGA TATTATGGAA 
GGGAATTTGC TGTCTCCGTC AGCCAAGCCT TCTCCCTCCA GTGATATGAT TGCACGTAAA 
GAATATATCA CTGCCAAGTA CGTGGAGCGG AGGTTTTCCA GAAAGATCTG TTCTACTGGG 
GCAGAAAAAC TGAATGAGCT ACTTGAGGCT GTGAAATCCA GGGACTTACT TGCACTAATC 
CAAGTCTATG CAGAAGGTGT AGAGATCATG GAACCACTGC TAGAATCTGG ACAGGAGCCA 
GGTGAAACTG CACTTCATTA TGCAGTCCGG ACAGCTGACC ACACGTCTCT TCCCTTGGTG 
GACTTCCTGG TACAAAACTG TGGAAATTTA GATAAGCAGA CTGGAAAGGG CAACACTGCT 
TTACACTACT GCTGCTTATA TAACAAGCCG GAATGCTTAA AATTGCTTTT GAGGGGAAAG 
CCAACTATTG ATGCTGTTAA CCATATTGGT GAGACGGCTT TTGATGTAGC AAAGAGACAG 
AAAGCAGCCC AGTGTGAGGA ACTGGTATCC CAAGCCCTGG CAGGGAAGCT GAATCCTCAT 
GTTCACGTGG AGTACGACTG GAACCTGCGT CAGGAAGAAA TGGATGAGAG CGACGATGAT 
TTGGAAGATA AGCCAAGCCC AATAAAGAAA GAGCGTTCCC CCAGACCCCA GAGCTTCTGC 
CATTCGTCCA GCATTTCCCC ACAAGACAAG CTGTCGCTGC CAGGTTTCAA TACTCCTCGG 
GACAAGCAGA GACTCTCTTA TGGCGCCTTT ACCAACCAAA TATTCATGTC TACAAGCACA 
GATTCCCCCA CGTCTCCTAC TGCAGATGCC CCTCCGCTGC CCCCTAGGAA CGCAGGCAAA 
GGTAATGACT TGGGCCCGAG TGCTGCAGAC AGAACTCTAA ATAAGTTTGA GAGATTGTCA 
CAGTCATCAG GCACAGGAAC AACAAAGACT GCATTAGGCC CAAGAGTCCT ACCAAAACTA 
CCTCAGAAAG TGGCACTTAG GAAAATAGAC ACCACTCATC ATCTCTCAAT GGATAAGTAT 
AACCAGCAAC CCGAAATCTT CCAGAAATCT CCCCAGCCTG GAGATCTGCC TCAGAAGCCA 
CAAATTGCAG ATCTTCCTCC GAAACCGCAG CCTTTGGATA TCGCCCAGAA ACCACACGTC 
GGAGATCTAC CTCCCAAGCC CGGAGAATTG CCTCCTAAAC CTCAGCTAGG AGATTTGCCC 
CCGAAACCGC AAGATTTACC TCCAAAGCCA CAGCTGAAAG ATCTTCCGCC CAAGCCCAAC 
CTGGCAGAGG TGATGTGCAA AACTCCTGTT GCGGATATAG TCCCCAAACC TCATACCAAT 
GAGGCCAATC AAAATTCCCA GCCGGTGGAT GCGACCCATA ATGCACAGCC CACGGACCAA 
ACCCCTGTCC AGAAGCAAGT TCCAGAAACT TCCAATAATG GCCCACCGCC TCTTCCAGAA 
ACACCCGTGC CACTTCCCCG AAAGATAAAC ACAGGGAAGA ATAAGCTTAA ACGAGTTAAA 
ACAATATACG ACTGCCAAGC GGACAATGAT GATGAACTCA CATTTGCGGA GGGGGAAGTG 
ATTATCGTCA CTGGAGAGGA GGACCAGGAG TGGTGGATTG GGCACATCGA AGGCTTCCCG 
GACAGGAAAG GAGTATTCCC TGTGTCCTTC GTCCACCTGT TGACTGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001090735.1
CDS	228..3398
Translation	MPDQISVSEFISETTEDYNSPTTSSFTTRLQSCRNSINVLEEALDQDRTALQKVKKSVKAIYNSGQDHVQNEENYAQALDKFGSNFLSRDNADLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGVKGDLKKPFDRAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDQKEDSQSKQAGYSMHQLQGNKEYGSEKKGYLLKKSDGIRKVWQRRKCTVKNGILTISHATSNRPPTKLNLLTCQVKPNADEKKCFDLISHNRTYHFQAEDEQEYIAWISVLTNSKEDALNMAFRGDQSTGESSLEDLTKAIIDDVQKTPGNEVCCDCGSPDPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDKLGTSELLLAKNVGNNSFNDIMEGNLLSPSAKPSPSSDMIARKEYITAKYVERRFSRKICSTGAEKLNELLEAVKSRDLLALIQVYAEGVEIMEPLLESGQEPGETALHYAVRTADHTSLPLVDFLVQNCGNLDKQTGKGNTALHYCCLYNKPECLKLLLRGKPTIDAVNHIGETAFDVAKRQKAAQCEELVSQALAGKLNPHVHVEYDWNLRQEEMDESDDDLEDKPSPIKKERSPRPQSFCHSSSISPQDKLSLPGFNTPRDKQRLSYGAFTNQIFMSTSTDSPTSPTADAPPLPPRNAGKGNDLGPSAADRTLNKFERLSQSSGTGTTKTALGPRVLPKLPQKVALRKIDTTHHLSMDKYNQQPEIFQKSPQPGDLPQKPQIADLPPKPQPLDIAQKPHVGDLPPKPGELPPKPQLGDLPPKPQDLPPKPQLKDLPPKPNLAEVMCKTPVADIVPKPHTNEANQNSQPVDATHNAQPTDQTPVQKQVPETSNNGPPPLPETPVPLPRKINTGKNKLKRVKTIYDCQADNDDELTFAEGEVIIVTGEEDQEWWIGHIEGFPDRKGVFPVSFVHLLTE

Target ORF information:

RefSeq Version	NM_001097266.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (asap1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001097266.1

ORF Insert Sequence:

ATGCCCGATC AGATCTCAGT ATCGGAGTTT ATATCGGAGA CCACAGAGGA TTACAATTCG 
CCAACCACAT CCAGTTTCAC GACCCGTCTT CAGAGCTGCA GGAATTCGAT CAATGTGCTG 
GAAGAGGCCT TAGATCAAGA TAGGACAGCG TTGCAGAAAG TGAAAAAATC GGTGAAAGCA 
ATTTATAATT CAGGCCAAGA TCATGTCCAA AATGAAGAAA ACTATGCACA AGCCTTAGAT 
AAATTTGGAA GTAATTTTCT AAGTCGGGAT AACGCAGATT TGGGCACCGC CTTCGTGAAA 
TTCTCCACTC TAACAAAAGA ACTATCAACG CTTCTTAAAA ACCTGCTACA GGGATTAAGC 
CACAATGTCA TCTTCACCTT GGATTCTCTC TTGAAAGGGG ACTTAAAGGG CGTAAAAGGG 
GACCTTAAGA AGCCATTCGA CAGAGCGTGG AAGGATTATG AAACCAAATT CACGAAAATC 
GAAAAGGAGA AAAGGGAACA CGCCAAGCAA CATGGGATGA TCCGTACGGA GATTACTGGG 
GCCGAGATTG CCGAAGAGAT GGAGAAGGAG AGGAGATTAT TCCAGCTACA GATGTGTGAG 
TATCTCATTA AAGTTAATGA AATCAAGACT AAGAAAGGCG TGGATCTGCT GCAGAACCTT 
ATAAAGTACT ATCACGCACA GTGCAATTTC TTCCAGGATG GATTGAAAAC AGCTGACAAG 
CTGAAGCAGT ACATAGAGAA ATTGGCTGCA GATTTGTACA ATATTAAGCA GACCCAAGAT 
GAGGAGAAGA AACAGCTCAC TGCCCTGCGA GACCTGATAA AATCCTCTTT ACAGCTGGAT 
CAGAAGGAGG ATTCCCAGAG TAAGCAGGCA GGCTACAGCA TGCACCAGCT CCAGGGGAAC 
AAGGAGTATG GCAGCGAGAA GAAAGGCTAC CTGCTGAAAA AAAGTGATGG CATCCGGAAA 
GTATGGCAGA GGAGGAAGTG CACTGTGAAA AATGGAATCC TGACCATTTC CCATGCAACG 
TCAAACAGGC CACCGACGAA GCTGAACCTG CTGACCTGCC AAGTCAAGCC GAACGCTGAT 
GAGAAGAAAT GCTTTGATCT CATCTCACAC AACAGAACAT ACCACTTCCA AGCAGAGGAC 
GAACAAGAAT ACATTGCGTG GATATCAGTT CTGACAAATA GCAAAGAAGA TGCACTCAAT 
ATGGCTTTCC GGGGAGACCA GAGCACTGGC GAGAGCAGCC TGGAGGATCT GACTAAGGCC 
ATTATAGACG ATGTACAGAA AACGCCTGGA AATGAAGTTT GCTGTGATTG TGGGTCACCG 
GACCCGACAT GGCTTTCCAC AAACCTTGGG ATTTTAACCT GTATAGAATG TTCGGGCATA 
CATAGGGAAA TGGGGGTTCA TATCTCTAGA ATCCAATCTC TGGAATTAGA CAAATTAGGG 
ACTTCTGAAC TCTTGCTGGC CAAGAATGTA GGAAACAATA GCTTCAATGA TATTATGGAA 
GGGAATTTGC TGTCTCCGTC AGCCAAGCCT TCTCCCTCCA GTGATATGAT TGCACGTAAA 
GAATATATCA CTGCCAAGTA CGTGGAGCGG AGGTTTTCCA GAAAGATCTG TTCTACTGGG 
GCAGAAAAAC TGAATGAGCT ACTTGAGGCT GTGAAATCCA GGGACTTACT TGCACTAATC 
CAAGTCTATG CAGAAGGTGT AGAGATCATG GAACCACTGC TAGAATCTGG ACAGGAGCCA 
GGTGAAACTG CACTTCATTA TGCAGTCCGG ACAGCTGACC ACACGTCTCT TCCCTTGGTG 
GACTTCCTGG TACAAAACTG TGGAAATTTA GATAAGCAGA CTGGAAAGGG CAACACTGCT 
TTACACTACT GCTGCTTATA TAACAAGCCG GAATGCTTAA AATTGCTTTT GAGGGGAAAG 
CCAACTATTG ATGCTGTTAA CCATATTGGT GAGACGGCTT TTGATGTAGC AAAGAGACAG 
AAAGCAGCCC AGTGTGAGGA ACTGGTATCC CAAGCCCTGG CAGGGAAGCT GAATCCTCAT 
GTTCACGTGG AGTACGACTG GAACCTGCGT CAGGAAGAAA TGGATGAGAG CGACGATGAT 
TTGGAAGATA AGCCAAGCCC AATAAAGAAA GAGCGTTCCC CCAGACCCCA GAGCTTCTGC 
CATTCGTCCA GCATTTCCCC ACAAGACAAG CTGTCGCTGC CAGGTTTCAA TACTCCTCGG 
GACAAGCAGA GACTCTCTTA TGGCGCCTTT ACCAACCAAA TATTCATGTC TACAAGCACA 
GATTCCCCCA CGTCTCCTAC TGCAGATGCC CCTCCGCTGC CCCCTAGGAA CGCAGGCAAA 
GGTAATGACT TGGGCCCGAG TGCTGCAGAC AGAACTCTAA ATAAGTTTGA GAGATTGTCA 
CAGTCATCAG GCACAGGAAC AACAAAGACT GCATTAGGCC CAAGAGTCCT ACCAAAACTA 
CCTCAGAAAG TGGCACTTAG GAAAATAGAC ACCACTCATC ATCTCTCAAT GGATAAGTAT 
AACCAGCAAC CCGAAATCTT CCAGAAATCT CCCCAGCCTG GAGATCTGCC TCAGAAGCCA 
CAAATTGCAG ATCTTCCTCC GAAACCGCAG CCTTTGGATA TCGCCCAGAA ACCACACGTC 
GGAGATCTAC CTCCCAAGCC CGGAGAATTG CCTCCTAAAC CTCAGCTAGG AGATTTGCCC 
CCGAAACCGC AAGATTTACC TCCAAAGCCA CAGCTGAAAG ATCTTCCGCC CAAGCCCAAC 
CTGGCAGAGG TGATGTGCAA AACTCCTGTT GCGGATATAG TCCCCAAACC TCATACCAAT 
GAGGCCAATC AAAATTCCCA GCCGGTGGAT GCGACCCATA ATGCACAGCC CACGGACCAA 
ACCCCTGTCC AGAAGCAAGT TCCAGAAACT TCCAATAATG GCCCACCGCC TCTTCCAGAA 
ACACCCGTGC CACTTCCCCG AAAGATAAAC ACAGGGAAGA ATAAGCTTAA ACGAGTTAAA 
ACAATATACG ACTGCCAAGC GGACAATGAT GATGAACTCA CATTTGCGGA GGGGGAAGTG 
ATTATCGTCA CTGGAGAGGA GGACCAGGAG TGGTGGATTG GGCACATCGA AGGCTTCCCG 
GACAGGAAAG GAGTATTCCC TGTGTCCTTC GTCCACCTGT TGACTGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

asap1 ( XM_012964131.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819585.1 Xenopus tropicalis ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (asap1), transcript variant X1, mRNA.

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CloneID	OXa29912
Clone ID Related Accession (Same CDS sequence)	XM_012964131.2
Accession Version	XM_012964131.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012964131.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGATCTT CGGCCTCTAG ACTTTCGAGT TTTTCCTCTA GAGACTCACT ATGGAACCGG 
ATGCCCGATC AGATCTCAGT ATCGGAGTTT ATATCGGAGA CCACAGAGGA TTACAATTCG 
CCAACCACGT CCAGTTTCAC GACCCGTCTT CAGAGCTGCA GGAATTCGAT CAATGTGCTG 
GAAGAGGCCT TAGATCAAGA TAGGACAGCG TTGCAGAAAG TGAAAAAATC GGTGAAAGCA 
ATTTATAATT CAGGCCAAGA TCATGTCCAA AATGAAGAAA ACTATGCACA AGCCTTAGAT 
AAATTTGGAA GTAATTTTCT AAGTCGGGAT AACGCAGATT TGGGCACCGC CTTCGTGAAA 
TTCTCCACTC TAACAAAAGA ACTATCAACG CTTCTTAAAA ACCTGCTACA GGGATTAAGC 
CACAATGTCA TCTTCACCTT GGATTCTCTC TTGAAAGGGG ACTTAAAGGG CGTAAAAGGG 
GACCTTAAGA AGCCATTCGA CAGAGCGTGG AAGGATTATG AAACCAAATT CACGAAAATC 
GAAAAGGAGA AAAGGGAACA CGCCAAGCAA CATGGGATGA TCCGTACGGA GATTACTGGG 
GCCGAGATTG CCGAAGAGAT GGAGAAGGAG AGGAGATTAT TCCAGCTACA GATGTGTGAG 
TATCTCATTA AAGTTAATGA AATCAAGACT AAGAAAGGCG TGGATCTGCT GCAGAACCTT 
ATAAAGTACT ATCACGCACA GTGCAATTTC TTCCAGGATG GATTGAAAAC AGCTGACAAG 
CTGAAGCAGT ACATAGAGAA ATTGGCTGCA GATTTGTACA ATATTAAGCA GACCCAAGAT 
GAGGAGAAGA AACAGCTCAC TGCCCTGCGA GACCTGATAA AATCCTCTTT ACAGCTGGAT 
CAGAAGGAGT CTAGAAGAGA TTCCCAGAGT AAGCAGGCAG GCTACAGCAT GCACCAGCTC 
CAGGGGAACA AGGAGTATGG CAGCGAGAAG AAAGGCTACC TGCTGAAAAA AAGTGATGGC 
ATCCGGAAAG TATGGCAGAG GAGGAAGTGC ACTGTGAAAA ATGGAATCCT GACCATTTCC 
CATGCAACGT CAAACAGGCC ACCGACGAAG CTGAACCTGC TGACCTGCCA AGTCAAGCCG 
AACGCTGATG AGAAGAAATG CTTTGATCTC ATCTCACACA ACAGAACATA CCACTTCCAA 
GCAGAGGACG AACAAGAATA CATTGCGTGG ATATCAGTTC TGACAAATAG CAAAGAAGAT 
GCACTCAATA TGGCTTTCCG GGGAGACCAG AGCACTGGCG AGAGCAGCCT GGAGGATCTG 
ACTAAGGCCA TTATAGACGA TGTACAGAAA ACGCCTGGAA ATGAAGTTTG CTGTGATTGT 
GGGTCACCGG ACCCGACATG GCTTTCCACA AACCTTGGGA TTTTAACCTG TATAGAATGT 
TCGGGCATAC ATAGGGAAAT GGGGGTTCAT ATCTCTAGAA TCCAATCTCT GGAATTAGAC 
AAATTAGGGA CTTCTGAACT CTTGCTGGCC AAGAATGTAG GAAACAATAG CTTCAATGAT 
ATTATGGAAG GGAATTTGCT GTCTCCGTCA GCCAAGCCTT CTCCCTCCAG TGATATGATT 
GCACGTAAAG AATATATCAC TGCCAAGTAC GTGGAGCGGA GGTTTTCCAG AAAGATCTGT 
TCTACTGGGG CAGAAAAACT GAATGAGCTA CTTGAGGCTG TGAAATCCAG GGACTTACTT 
GCACTAATCC AAGTCTATGC AGAAGGTGTA GAGATCATGG AACCACTGCT AGAATCTGGA 
CAGGAGCCAG GTGAAACTGC ACTTCATTAT GCAGTCCGGA CAGCTGACCA CACGTCTCTT 
CCCTTGGTGG ACTTCCTGGT ACAAAACTGT GGAAATTTAG ATAAGCAGAC TGGAAAGGGC 
AACACTGCTT TACACTACTG CTGCTTATAT AACAAGCCGG AATGCTTAAA ATTGCTTTTG 
AGGGGAAAGC CAACTATTGA TGCTGTTAAC CATATTGGTG AGACGGCTTT TGATGTAGCA 
AAGAGACAGA AAGCAGCCCA GTGTGAGGAA CTGGTATCCC AAGCCCTGGC AGGGAAGCTG 
AATCCTCATG TTCACGTGGA GTACGACTGG AACCTGCGTC AGGAAGAAAT GGATGAGAGC 
GACGATGATT TGGAAGATAA GCCAAGCCCA ATAAAGAAAG AGCGTTCCCC CAGACCCCAG 
AGCTTCTGCC ATTCGTCCAG CATTTCCCCA CAAGACAAGC TGTCGCTGCC AGGTTTCAAT 
ACTCCTCGGG ACAAGCAGAG ACTCTCTTAT GGCGCCTTTA CCAACCAAAT ATTCATGTCT 
ACAAGCACAG ATTCCCCCAC GTCTCCTACT GCAGATGCCC CTCCGCTGCC CCCTAGGAAC 
GCAGGCAAAG TGGCGAGTGG CCCTACTTCA ACCCTCCCTC TAAGCACCCA ATCCCCTAGT 
GGCAGTTCCA CTCTGTCCAA GAAGAGACCT CCTCCCCCAC CCCCGGGACA CAAACGAACC 
CTCTCCGACC CACCAAGTCC GCTACCTCAT GGCCCCCCCA GTAAGGGCTC AGTTGTTTGT 
GGTAATGACT TGGGCCCGAG TGCTGCAGAC AGAACTCTAA ATAAGTTTGA GAGATTGTCA 
CAGTCATCAG GCACAGGAAC AACAAAGACT GCATTAGGCC CAAGAGTCCT ACCAAAACTA 
CCTCAGAAAG TGGCACTTAG GAAAATAGAC ACCACTCATC ATCTCTCAAT GGATAAGTAT 
AACCAGCAAC CCGAAATCTT CCAGAAATCT CCCCAGCCTG GAGATCTGCC TCAGAAGCCA 
CAAATTGCAG ATCTTCCTCC GAAACCGCAG CCTTTGGATA TCGCCCAGAA ACCACACGTC 
GGAGATCTAC CTCCCAAGCC CGGAGAATTG CCTCCTAAAC CTCAGCTAGG AGATTTGCCC 
CCGAAACCGC AAGATTTACC TCCAAAGCCA CAGCTGAAAG ATCTTCCGCC CAAGCCCAAC 
CTGGCAGAGG TGATGTGCAA AACTCCTGTT GCGGATATAG TCCCCAAACC TCATACCAAT 
GAGGCCAATC AAAATTCCCA GCCGGTGGAT GCGACCCATA ATGCACAGCC CACGGACCAA 
ACCCCTGTCC AGAAGCAAGT TCCAGAAACT TCCAATAATG GCCCACCGCC TCTTCCAGAA 
ACACCCGTGC CACTTCCCCG AAAGATAAAC ACAGGGAAGA ATAAGCTTAA ACGAGTTAAA 
ACAATATACG ACTGCCAAGC GGACAATGAT GATGAACTCA CATTTGCGGA GGGGGAAGTG 
ATTATCGTCA CTGGAGAGGA GGACCAGGAG TGGTGGATTG GGCACATCGA AGGCTTCCCG 
GACAGGAAAG GAGTATTCCC TGTGTCCTTC GTCCACCTGT TGACTGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819585.1
CDS	30..3440
Translation	MRSSASRLSSFSSRDSLWNRMPDQISVSEFISETTEDYNSPTTSSFTTRLQSCRNSINVLEEALDQDRTALQKVKKSVKAIYNSGQDHVQNEENYAQALDKFGSNFLSRDNADLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGVKGDLKKPFDRAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDQKESRRDSQSKQAGYSMHQLQGNKEYGSEKKGYLLKKSDGIRKVWQRRKCTVKNGILTISHATSNRPPTKLNLLTCQVKPNADEKKCFDLISHNRTYHFQAEDEQEYIAWISVLTNSKEDALNMAFRGDQSTGESSLEDLTKAIIDDVQKTPGNEVCCDCGSPDPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDKLGTSELLLAKNVGNNSFNDIMEGNLLSPSAKPSPSSDMIARKEYITAKYVERRFSRKICSTGAEKLNELLEAVKSRDLLALIQVYAEGVEIMEPLLESGQEPGETALHYAVRTADHTSLPLVDFLVQNCGNLDKQTGKGNTALHYCCLYNKPECLKLLLRGKPTIDAVNHIGETAFDVAKRQKAAQCEELVSQALAGKLNPHVHVEYDWNLRQEEMDESDDDLEDKPSPIKKERSPRPQSFCHSSSISPQDKLSLPGFNTPRDKQRLSYGAFTNQIFMSTSTDSPTSPTADAPPLPPRNAGKVASGPTSTLPLSTQSPSGSSTLSKKRPPPPPPGHKRTLSDPPSPLPHGPPSKGSVVCGNDLGPSAADRTLNKFERLSQSSGTGTTKTALGPRVLPKLPQKVALRKIDTTHHLSMDKYNQQPEIFQKSPQPGDLPQKPQIADLPPKPQPLDIAQKPHVGDLPPKPGELPPKPQLGDLPPKPQDLPPKPQLKDLPPKPNLAEVMCKTPVADIVPKPHTNEANQNSQPVDATHNAQPTDQTPVQKQVPETSNNGPPPLPETPVPLPRKINTGKNKLKRVKTIYDCQADNDDELTFAEGEVIIVTGEEDQEWWIGHIEGFPDRKGVFPVSFVHLLTE

Target ORF information:

RefSeq Version	XM_012964131.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (asap1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012964131.2

ORF Insert Sequence:

ATGCGATCTT CGGCCTCTAG ACTTTCGAGT TTTTCCTCTA GAGACTCACT ATGGAACCGG 
ATGCCCGATC AGATCTCAGT ATCGGAGTTT ATATCGGAGA CCACAGAGGA TTACAATTCG 
CCAACCACGT CCAGTTTCAC GACCCGTCTT CAGAGCTGCA GGAATTCGAT CAATGTGCTG 
GAAGAGGCCT TAGATCAAGA TAGGACAGCG TTGCAGAAAG TGAAAAAATC GGTGAAAGCA 
ATTTATAATT CAGGCCAAGA TCATGTCCAA AATGAAGAAA ACTATGCACA AGCCTTAGAT 
AAATTTGGAA GTAATTTTCT AAGTCGGGAT AACGCAGATT TGGGCACCGC CTTCGTGAAA 
TTCTCCACTC TAACAAAAGA ACTATCAACG CTTCTTAAAA ACCTGCTACA GGGATTAAGC 
CACAATGTCA TCTTCACCTT GGATTCTCTC TTGAAAGGGG ACTTAAAGGG CGTAAAAGGG 
GACCTTAAGA AGCCATTCGA CAGAGCGTGG AAGGATTATG AAACCAAATT CACGAAAATC 
GAAAAGGAGA AAAGGGAACA CGCCAAGCAA CATGGGATGA TCCGTACGGA GATTACTGGG 
GCCGAGATTG CCGAAGAGAT GGAGAAGGAG AGGAGATTAT TCCAGCTACA GATGTGTGAG 
TATCTCATTA AAGTTAATGA AATCAAGACT AAGAAAGGCG TGGATCTGCT GCAGAACCTT 
ATAAAGTACT ATCACGCACA GTGCAATTTC TTCCAGGATG GATTGAAAAC AGCTGACAAG 
CTGAAGCAGT ACATAGAGAA ATTGGCTGCA GATTTGTACA ATATTAAGCA GACCCAAGAT 
GAGGAGAAGA AACAGCTCAC TGCCCTGCGA GACCTGATAA AATCCTCTTT ACAGCTGGAT 
CAGAAGGAGT CTAGAAGAGA TTCCCAGAGT AAGCAGGCAG GCTACAGCAT GCACCAGCTC 
CAGGGGAACA AGGAGTATGG CAGCGAGAAG AAAGGCTACC TGCTGAAAAA AAGTGATGGC 
ATCCGGAAAG TATGGCAGAG GAGGAAGTGC ACTGTGAAAA ATGGAATCCT GACCATTTCC 
CATGCAACGT CAAACAGGCC ACCGACGAAG CTGAACCTGC TGACCTGCCA AGTCAAGCCG 
AACGCTGATG AGAAGAAATG CTTTGATCTC ATCTCACACA ACAGAACATA CCACTTCCAA 
GCAGAGGACG AACAAGAATA CATTGCGTGG ATATCAGTTC TGACAAATAG CAAAGAAGAT 
GCACTCAATA TGGCTTTCCG GGGAGACCAG AGCACTGGCG AGAGCAGCCT GGAGGATCTG 
ACTAAGGCCA TTATAGACGA TGTACAGAAA ACGCCTGGAA ATGAAGTTTG CTGTGATTGT 
GGGTCACCGG ACCCGACATG GCTTTCCACA AACCTTGGGA TTTTAACCTG TATAGAATGT 
TCGGGCATAC ATAGGGAAAT GGGGGTTCAT ATCTCTAGAA TCCAATCTCT GGAATTAGAC 
AAATTAGGGA CTTCTGAACT CTTGCTGGCC AAGAATGTAG GAAACAATAG CTTCAATGAT 
ATTATGGAAG GGAATTTGCT GTCTCCGTCA GCCAAGCCTT CTCCCTCCAG TGATATGATT 
GCACGTAAAG AATATATCAC TGCCAAGTAC GTGGAGCGGA GGTTTTCCAG AAAGATCTGT 
TCTACTGGGG CAGAAAAACT GAATGAGCTA CTTGAGGCTG TGAAATCCAG GGACTTACTT 
GCACTAATCC AAGTCTATGC AGAAGGTGTA GAGATCATGG AACCACTGCT AGAATCTGGA 
CAGGAGCCAG GTGAAACTGC ACTTCATTAT GCAGTCCGGA CAGCTGACCA CACGTCTCTT 
CCCTTGGTGG ACTTCCTGGT ACAAAACTGT GGAAATTTAG ATAAGCAGAC TGGAAAGGGC 
AACACTGCTT TACACTACTG CTGCTTATAT AACAAGCCGG AATGCTTAAA ATTGCTTTTG 
AGGGGAAAGC CAACTATTGA TGCTGTTAAC CATATTGGTG AGACGGCTTT TGATGTAGCA 
AAGAGACAGA AAGCAGCCCA GTGTGAGGAA CTGGTATCCC AAGCCCTGGC AGGGAAGCTG 
AATCCTCATG TTCACGTGGA GTACGACTGG AACCTGCGTC AGGAAGAAAT GGATGAGAGC 
GACGATGATT TGGAAGATAA GCCAAGCCCA ATAAAGAAAG AGCGTTCCCC CAGACCCCAG 
AGCTTCTGCC ATTCGTCCAG CATTTCCCCA CAAGACAAGC TGTCGCTGCC AGGTTTCAAT 
ACTCCTCGGG ACAAGCAGAG ACTCTCTTAT GGCGCCTTTA CCAACCAAAT ATTCATGTCT 
ACAAGCACAG ATTCCCCCAC GTCTCCTACT GCAGATGCCC CTCCGCTGCC CCCTAGGAAC 
GCAGGCAAAG TGGCGAGTGG CCCTACTTCA ACCCTCCCTC TAAGCACCCA ATCCCCTAGT 
GGCAGTTCCA CTCTGTCCAA GAAGAGACCT CCTCCCCCAC CCCCGGGACA CAAACGAACC 
CTCTCCGACC CACCAAGTCC GCTACCTCAT GGCCCCCCCA GTAAGGGCTC AGTTGTTTGT 
GGTAATGACT TGGGCCCGAG TGCTGCAGAC AGAACTCTAA ATAAGTTTGA GAGATTGTCA 
CAGTCATCAG GCACAGGAAC AACAAAGACT GCATTAGGCC CAAGAGTCCT ACCAAAACTA 
CCTCAGAAAG TGGCACTTAG GAAAATAGAC ACCACTCATC ATCTCTCAAT GGATAAGTAT 
AACCAGCAAC CCGAAATCTT CCAGAAATCT CCCCAGCCTG GAGATCTGCC TCAGAAGCCA 
CAAATTGCAG ATCTTCCTCC GAAACCGCAG CCTTTGGATA TCGCCCAGAA ACCACACGTC 
GGAGATCTAC CTCCCAAGCC CGGAGAATTG CCTCCTAAAC CTCAGCTAGG AGATTTGCCC 
CCGAAACCGC AAGATTTACC TCCAAAGCCA CAGCTGAAAG ATCTTCCGCC CAAGCCCAAC 
CTGGCAGAGG TGATGTGCAA AACTCCTGTT GCGGATATAG TCCCCAAACC TCATACCAAT 
GAGGCCAATC AAAATTCCCA GCCGGTGGAT GCGACCCATA ATGCACAGCC CACGGACCAA 
ACCCCTGTCC AGAAGCAAGT TCCAGAAACT TCCAATAATG GCCCACCGCC TCTTCCAGAA 
ACACCCGTGC CACTTCCCCG AAAGATAAAC ACAGGGAAGA ATAAGCTTAA ACGAGTTAAA 
ACAATATACG ACTGCCAAGC GGACAATGAT GATGAACTCA CATTTGCGGA GGGGGAAGTG 
ATTATCGTCA CTGGAGAGGA GGACCAGGAG TGGTGGATTG GGCACATCGA AGGCTTCCCG 
GACAGGAAAG GAGTATTCCC TGTGTCCTTC GTCCACCTGT TGACTGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

asap1 ( XM_012964132.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819586.1 Xenopus tropicalis ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (asap1), transcript variant X2, mRNA.

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CloneID	OXa29913
Clone ID Related Accession (Same CDS sequence)	XM_012964132.2
Accession Version	XM_012964132.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3402bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012964132.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGATCTT CGGCCTCTAG ACTTTCGAGT TTTTCCTCTA GAGACTCACT ATGGAACCGG 
ATGCCCGATC AGATCTCAGT ATCGGAGTTT ATATCGGAGA CCACAGAGGA TTACAATTCG 
CCAACCACGT CCAGTTTCAC GACCCGTCTT CAGAGCTGCA GGAATTCGAT CAATGTGCTG 
GAAGAGGCCT TAGATCAAGA TAGGACAGCG TTGCAGAAAG TGAAAAAATC GGTGAAAGCA 
ATTTATAATT CAGGCCAAGA TCATGTCCAA AATGAAGAAA ACTATGCACA AGCCTTAGAT 
AAATTTGGAA GTAATTTTCT AAGTCGGGAT AACGCAGATT TGGGCACCGC CTTCGTGAAA 
TTCTCCACTC TAACAAAAGA ACTATCAACG CTTCTTAAAA ACCTGCTACA GGGATTAAGC 
CACAATGTCA TCTTCACCTT GGATTCTCTC TTGAAAGGGG ACTTAAAGGG CGTAAAAGGG 
GACCTTAAGA AGCCATTCGA CAGAGCGTGG AAGGATTATG AAACCAAATT CACGAAAATC 
GAAAAGGAGA AAAGGGAACA CGCCAAGCAA CATGGGATGA TCCGTACGGA GATTACTGGG 
GCCGAGATTG CCGAAGAGAT GGAGAAGGAG AGGAGATTAT TCCAGCTACA GATGTGTGAG 
TATCTCATTA AAGTTAATGA AATCAAGACT AAGAAAGGCG TGGATCTGCT GCAGAACCTT 
ATAAAGTACT ATCACGCACA GTGCAATTTC TTCCAGGATG GATTGAAAAC AGCTGACAAG 
CTGAAGCAGT ACATAGAGAA ATTGGCTGCA GATTTGTACA ATATTAAGCA GACCCAAGAT 
GAGGAGAAGA AACAGCTCAC TGCCCTGCGA GACCTGATAA AATCCTCTTT ACAGCTGGAT 
CAGAAGGAGG ATTCCCAGAG TAAGCAGGCA GGCTACAGCA TGCACCAGCT CCAGGGGAAC 
AAGGAGTATG GCAGCGAGAA GAAAGGCTAC CTGCTGAAAA AAAGTGATGG CATCCGGAAA 
GTATGGCAGA GGAGGAAGTG CACTGTGAAA AATGGAATCC TGACCATTTC CCATGCAACG 
TCAAACAGGC CACCGACGAA GCTGAACCTG CTGACCTGCC AAGTCAAGCC GAACGCTGAT 
GAGAAGAAAT GCTTTGATCT CATCTCACAC AACAGAACAT ACCACTTCCA AGCAGAGGAC 
GAACAAGAAT ACATTGCGTG GATATCAGTT CTGACAAATA GCAAAGAAGA TGCACTCAAT 
ATGGCTTTCC GGGGAGACCA GAGCACTGGC GAGAGCAGCC TGGAGGATCT GACTAAGGCC 
ATTATAGACG ATGTACAGAA AACGCCTGGA AATGAAGTTT GCTGTGATTG TGGGTCACCG 
GACCCGACAT GGCTTTCCAC AAACCTTGGG ATTTTAACCT GTATAGAATG TTCGGGCATA 
CATAGGGAAA TGGGGGTTCA TATCTCTAGA ATCCAATCTC TGGAATTAGA CAAATTAGGG 
ACTTCTGAAC TCTTGCTGGC CAAGAATGTA GGAAACAATA GCTTCAATGA TATTATGGAA 
GGGAATTTGC TGTCTCCGTC AGCCAAGCCT TCTCCCTCCA GTGATATGAT TGCACGTAAA 
GAATATATCA CTGCCAAGTA CGTGGAGCGG AGGTTTTCCA GAAAGATCTG TTCTACTGGG 
GCAGAAAAAC TGAATGAGCT ACTTGAGGCT GTGAAATCCA GGGACTTACT TGCACTAATC 
CAAGTCTATG CAGAAGGTGT AGAGATCATG GAACCACTGC TAGAATCTGG ACAGGAGCCA 
GGTGAAACTG CACTTCATTA TGCAGTCCGG ACAGCTGACC ACACGTCTCT TCCCTTGGTG 
GACTTCCTGG TACAAAACTG TGGAAATTTA GATAAGCAGA CTGGAAAGGG CAACACTGCT 
TTACACTACT GCTGCTTATA TAACAAGCCG GAATGCTTAA AATTGCTTTT GAGGGGAAAG 
CCAACTATTG ATGCTGTTAA CCATATTGGT GAGACGGCTT TTGATGTAGC AAAGAGACAG 
AAAGCAGCCC AGTGTGAGGA ACTGGTATCC CAAGCCCTGG CAGGGAAGCT GAATCCTCAT 
GTTCACGTGG AGTACGACTG GAACCTGCGT CAGGAAGAAA TGGATGAGAG CGACGATGAT 
TTGGAAGATA AGCCAAGCCC AATAAAGAAA GAGCGTTCCC CCAGACCCCA GAGCTTCTGC 
CATTCGTCCA GCATTTCCCC ACAAGACAAG CTGTCGCTGC CAGGTTTCAA TACTCCTCGG 
GACAAGCAGA GACTCTCTTA TGGCGCCTTT ACCAACCAAA TATTCATGTC TACAAGCACA 
GATTCCCCCA CGTCTCCTAC TGCAGATGCC CCTCCGCTGC CCCCTAGGAA CGCAGGCAAA 
GTGGCGAGTG GCCCTACTTC AACCCTCCCT CTAAGCACCC AATCCCCTAG TGGCAGTTCC 
ACTCTGTCCA AGAAGAGACC TCCTCCCCCA CCCCCGGGAC ACAAACGAAC CCTCTCCGAC 
CCACCAAGTC CGCTACCTCA TGGCCCCCCC AGTAAGGGCT CAGTTGTTTG TGGTAATGAC 
TTGGGCCCGA GTGCTGCAGA CAGAACTCTA AATAAGTTTG AGAGATTGTC ACAGTCATCA 
GGCACAGGAA CAACAAAGAC TGCATTAGGC CCAAGAGTCC TACCAAAACT ACCTCAGAAA 
GTGGCACTTA GGAAAATAGA CACCACTCAT CATCTCTCAA TGGATAAGTA TAACCAGCAA 
CCCGAAATCT TCCAGAAATC TCCCCAGCCT GGAGATCTGC CTCAGAAGCC ACAAATTGCA 
GATCTTCCTC CGAAACCGCA GCCTTTGGAT ATCGCCCAGA AACCACACGT CGGAGATCTA 
CCTCCCAAGC CCGGAGAATT GCCTCCTAAA CCTCAGCTAG GAGATTTGCC CCCGAAACCG 
CAAGATTTAC CTCCAAAGCC ACAGCTGAAA GATCTTCCGC CCAAGCCCAA CCTGGCAGAG 
GTGATGTGCA AAACTCCTGT TGCGGATATA GTCCCCAAAC CTCATACCAA TGAGGCCAAT 
CAAAATTCCC AGCCGGTGGA TGCGACCCAT AATGCACAGC CCACGGACCA AACCCCTGTC 
CAGAAGCAAG TTCCAGAAAC TTCCAATAAT GGCCCACCGC CTCTTCCAGA AACACCCGTG 
CCACTTCCCC GAAAGATAAA CACAGGGAAG AATAAGCTTA AACGAGTTAA AACAATATAC 
GACTGCCAAG CGGACAATGA TGATGAACTC ACATTTGCGG AGGGGGAAGT GATTATCGTC 
ACTGGAGAGG AGGACCAGGA GTGGTGGATT GGGCACATCG AAGGCTTCCC GGACAGGAAA 
GGAGTATTCC CTGTGTCCTT CGTCCACCTG TTGACTGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819586.1
CDS	30..3431
Translation	MRSSASRLSSFSSRDSLWNRMPDQISVSEFISETTEDYNSPTTSSFTTRLQSCRNSINVLEEALDQDRTALQKVKKSVKAIYNSGQDHVQNEENYAQALDKFGSNFLSRDNADLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGVKGDLKKPFDRAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDQKEDSQSKQAGYSMHQLQGNKEYGSEKKGYLLKKSDGIRKVWQRRKCTVKNGILTISHATSNRPPTKLNLLTCQVKPNADEKKCFDLISHNRTYHFQAEDEQEYIAWISVLTNSKEDALNMAFRGDQSTGESSLEDLTKAIIDDVQKTPGNEVCCDCGSPDPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDKLGTSELLLAKNVGNNSFNDIMEGNLLSPSAKPSPSSDMIARKEYITAKYVERRFSRKICSTGAEKLNELLEAVKSRDLLALIQVYAEGVEIMEPLLESGQEPGETALHYAVRTADHTSLPLVDFLVQNCGNLDKQTGKGNTALHYCCLYNKPECLKLLLRGKPTIDAVNHIGETAFDVAKRQKAAQCEELVSQALAGKLNPHVHVEYDWNLRQEEMDESDDDLEDKPSPIKKERSPRPQSFCHSSSISPQDKLSLPGFNTPRDKQRLSYGAFTNQIFMSTSTDSPTSPTADAPPLPPRNAGKVASGPTSTLPLSTQSPSGSSTLSKKRPPPPPPGHKRTLSDPPSPLPHGPPSKGSVVCGNDLGPSAADRTLNKFERLSQSSGTGTTKTALGPRVLPKLPQKVALRKIDTTHHLSMDKYNQQPEIFQKSPQPGDLPQKPQIADLPPKPQPLDIAQKPHVGDLPPKPGELPPKPQLGDLPPKPQDLPPKPQLKDLPPKPNLAEVMCKTPVADIVPKPHTNEANQNSQPVDATHNAQPTDQTPVQKQVPETSNNGPPPLPETPVPLPRKINTGKNKLKRVKTIYDCQADNDDELTFAEGEVIIVTGEEDQEWWIGHIEGFPDRKGVFPVSFVHLLTE

Target ORF information:

RefSeq Version	XM_012964132.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (asap1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012964132.2

ORF Insert Sequence:

ATGCGATCTT CGGCCTCTAG ACTTTCGAGT TTTTCCTCTA GAGACTCACT ATGGAACCGG 
ATGCCCGATC AGATCTCAGT ATCGGAGTTT ATATCGGAGA CCACAGAGGA TTACAATTCG 
CCAACCACGT CCAGTTTCAC GACCCGTCTT CAGAGCTGCA GGAATTCGAT CAATGTGCTG 
GAAGAGGCCT TAGATCAAGA TAGGACAGCG TTGCAGAAAG TGAAAAAATC GGTGAAAGCA 
ATTTATAATT CAGGCCAAGA TCATGTCCAA AATGAAGAAA ACTATGCACA AGCCTTAGAT 
AAATTTGGAA GTAATTTTCT AAGTCGGGAT AACGCAGATT TGGGCACCGC CTTCGTGAAA 
TTCTCCACTC TAACAAAAGA ACTATCAACG CTTCTTAAAA ACCTGCTACA GGGATTAAGC 
CACAATGTCA TCTTCACCTT GGATTCTCTC TTGAAAGGGG ACTTAAAGGG CGTAAAAGGG 
GACCTTAAGA AGCCATTCGA CAGAGCGTGG AAGGATTATG AAACCAAATT CACGAAAATC 
GAAAAGGAGA AAAGGGAACA CGCCAAGCAA CATGGGATGA TCCGTACGGA GATTACTGGG 
GCCGAGATTG CCGAAGAGAT GGAGAAGGAG AGGAGATTAT TCCAGCTACA GATGTGTGAG 
TATCTCATTA AAGTTAATGA AATCAAGACT AAGAAAGGCG TGGATCTGCT GCAGAACCTT 
ATAAAGTACT ATCACGCACA GTGCAATTTC TTCCAGGATG GATTGAAAAC AGCTGACAAG 
CTGAAGCAGT ACATAGAGAA ATTGGCTGCA GATTTGTACA ATATTAAGCA GACCCAAGAT 
GAGGAGAAGA AACAGCTCAC TGCCCTGCGA GACCTGATAA AATCCTCTTT ACAGCTGGAT 
CAGAAGGAGG ATTCCCAGAG TAAGCAGGCA GGCTACAGCA TGCACCAGCT CCAGGGGAAC 
AAGGAGTATG GCAGCGAGAA GAAAGGCTAC CTGCTGAAAA AAAGTGATGG CATCCGGAAA 
GTATGGCAGA GGAGGAAGTG CACTGTGAAA AATGGAATCC TGACCATTTC CCATGCAACG 
TCAAACAGGC CACCGACGAA GCTGAACCTG CTGACCTGCC AAGTCAAGCC GAACGCTGAT 
GAGAAGAAAT GCTTTGATCT CATCTCACAC AACAGAACAT ACCACTTCCA AGCAGAGGAC 
GAACAAGAAT ACATTGCGTG GATATCAGTT CTGACAAATA GCAAAGAAGA TGCACTCAAT 
ATGGCTTTCC GGGGAGACCA GAGCACTGGC GAGAGCAGCC TGGAGGATCT GACTAAGGCC 
ATTATAGACG ATGTACAGAA AACGCCTGGA AATGAAGTTT GCTGTGATTG TGGGTCACCG 
GACCCGACAT GGCTTTCCAC AAACCTTGGG ATTTTAACCT GTATAGAATG TTCGGGCATA 
CATAGGGAAA TGGGGGTTCA TATCTCTAGA ATCCAATCTC TGGAATTAGA CAAATTAGGG 
ACTTCTGAAC TCTTGCTGGC CAAGAATGTA GGAAACAATA GCTTCAATGA TATTATGGAA 
GGGAATTTGC TGTCTCCGTC AGCCAAGCCT TCTCCCTCCA GTGATATGAT TGCACGTAAA 
GAATATATCA CTGCCAAGTA CGTGGAGCGG AGGTTTTCCA GAAAGATCTG TTCTACTGGG 
GCAGAAAAAC TGAATGAGCT ACTTGAGGCT GTGAAATCCA GGGACTTACT TGCACTAATC 
CAAGTCTATG CAGAAGGTGT AGAGATCATG GAACCACTGC TAGAATCTGG ACAGGAGCCA 
GGTGAAACTG CACTTCATTA TGCAGTCCGG ACAGCTGACC ACACGTCTCT TCCCTTGGTG 
GACTTCCTGG TACAAAACTG TGGAAATTTA GATAAGCAGA CTGGAAAGGG CAACACTGCT 
TTACACTACT GCTGCTTATA TAACAAGCCG GAATGCTTAA AATTGCTTTT GAGGGGAAAG 
CCAACTATTG ATGCTGTTAA CCATATTGGT GAGACGGCTT TTGATGTAGC AAAGAGACAG 
AAAGCAGCCC AGTGTGAGGA ACTGGTATCC CAAGCCCTGG CAGGGAAGCT GAATCCTCAT 
GTTCACGTGG AGTACGACTG GAACCTGCGT CAGGAAGAAA TGGATGAGAG CGACGATGAT 
TTGGAAGATA AGCCAAGCCC AATAAAGAAA GAGCGTTCCC CCAGACCCCA GAGCTTCTGC 
CATTCGTCCA GCATTTCCCC ACAAGACAAG CTGTCGCTGC CAGGTTTCAA TACTCCTCGG 
GACAAGCAGA GACTCTCTTA TGGCGCCTTT ACCAACCAAA TATTCATGTC TACAAGCACA 
GATTCCCCCA CGTCTCCTAC TGCAGATGCC CCTCCGCTGC CCCCTAGGAA CGCAGGCAAA 
GTGGCGAGTG GCCCTACTTC AACCCTCCCT CTAAGCACCC AATCCCCTAG TGGCAGTTCC 
ACTCTGTCCA AGAAGAGACC TCCTCCCCCA CCCCCGGGAC ACAAACGAAC CCTCTCCGAC 
CCACCAAGTC CGCTACCTCA TGGCCCCCCC AGTAAGGGCT CAGTTGTTTG TGGTAATGAC 
TTGGGCCCGA GTGCTGCAGA CAGAACTCTA AATAAGTTTG AGAGATTGTC ACAGTCATCA 
GGCACAGGAA CAACAAAGAC TGCATTAGGC CCAAGAGTCC TACCAAAACT ACCTCAGAAA 
GTGGCACTTA GGAAAATAGA CACCACTCAT CATCTCTCAA TGGATAAGTA TAACCAGCAA 
CCCGAAATCT TCCAGAAATC TCCCCAGCCT GGAGATCTGC CTCAGAAGCC ACAAATTGCA 
GATCTTCCTC CGAAACCGCA GCCTTTGGAT ATCGCCCAGA AACCACACGT CGGAGATCTA 
CCTCCCAAGC CCGGAGAATT GCCTCCTAAA CCTCAGCTAG GAGATTTGCC CCCGAAACCG 
CAAGATTTAC CTCCAAAGCC ACAGCTGAAA GATCTTCCGC CCAAGCCCAA CCTGGCAGAG 
GTGATGTGCA AAACTCCTGT TGCGGATATA GTCCCCAAAC CTCATACCAA TGAGGCCAAT 
CAAAATTCCC AGCCGGTGGA TGCGACCCAT AATGCACAGC CCACGGACCA AACCCCTGTC 
CAGAAGCAAG TTCCAGAAAC TTCCAATAAT GGCCCACCGC CTCTTCCAGA AACACCCGTG 
CCACTTCCCC GAAAGATAAA CACAGGGAAG AATAAGCTTA AACGAGTTAA AACAATATAC 
GACTGCCAAG CGGACAATGA TGATGAACTC ACATTTGCGG AGGGGGAAGT GATTATCGTC 
ACTGGAGAGG AGGACCAGGA GTGGTGGATT GGGCACATCG AAGGCTTCCC GGACAGGAAA 
GGAGTATTCC CTGTGTCCTT CGTCCACCTG TTGACTGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

asap1 ( XM_012964133.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819587.1 Xenopus tropicalis ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (asap1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa29914
Clone ID Related Accession (Same CDS sequence)	XM_012964133.2
Accession Version	XM_012964133.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012964133.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCCCG ATCAGATCTC AGTATCGGAG TTTATATCGG AGACCACAGA GGATTACAAT 
TCGCCAACCA CGTCCAGTTT CACGACCCGT CTTCAGAGCT GCAGGAATTC GATCAATGTG 
CTGGAAGAGG CCTTAGATCA AGATAGGACA GCGTTGCAGA AAGTGAAAAA ATCGGTGAAA 
GCAATTTATA ATTCAGGCCA AGATCATGTC CAAAATGAAG AAAACTATGC ACAAGCCTTA 
GATAAATTTG GAAGTAATTT TCTAAGTCGG GATAACGCAG ATTTGGGCAC CGCCTTCGTG 
AAATTCTCCA CTCTAACAAA AGAACTATCA ACGCTTCTTA AAAACCTGCT ACAGGGATTA 
AGCCACAATG TCATCTTCAC CTTGGATTCT CTCTTGAAAG GGGACTTAAA GGGCGTAAAA 
GGGGACCTTA AGAAGCCATT CGACAGAGCG TGGAAGGATT ATGAAACCAA ATTCACGAAA 
ATCGAAAAGG AGAAAAGGGA ACACGCCAAG CAACATGGGA TGATCCGTAC GGAGATTACT 
GGGGCCGAGA TTGCCGAAGA GATGGAGAAG GAGAGGAGAT TATTCCAGCT ACAGATGTGT 
GAGTATCTCA TTAAAGTTAA TGAAATCAAG ACTAAGAAAG GCGTGGATCT GCTGCAGAAC 
CTTATAAAGT ACTATCACGC ACAGTGCAAT TTCTTCCAGG ATGGATTGAA AACAGCTGAC 
AAGCTGAAGC AGTACATAGA GAAATTGGCT GCAGATTTGT ACAATATTAA GCAGACCCAA 
GATGAGGAGA AGAAACAGCT CACTGCCCTG CGAGACCTGA TAAAATCCTC TTTACAGCTG 
GATCAGAAGG AGTCTAGAAG AGATTCCCAG AGTAAGCAGG CAGGCTACAG CATGCACCAG 
CTCCAGGGGA ACAAGGAGTA TGGCAGCGAG AAGAAAGGCT ACCTGCTGAA AAAAAGTGAT 
GGCATCCGGA AAGTATGGCA GAGGAGGAAG TGCACTGTGA AAAATGGAAT CCTGACCATT 
TCCCATGCAA CGTCAAACAG GCCACCGACG AAGCTGAACC TGCTGACCTG CCAAGTCAAG 
CCGAACGCTG ATGAGAAGAA ATGCTTTGAT CTCATCTCAC ACAACAGAAC ATACCACTTC 
CAAGCAGAGG ACGAACAAGA ATACATTGCG TGGATATCAG TTCTGACAAA TAGCAAAGAA 
GATGCACTCA ATATGGCTTT CCGGGGAGAC CAGAGCACTG GCGAGAGCAG CCTGGAGGAT 
CTGACTAAGG CCATTATAGA CGATGTACAG AAAACGCCTG GAAATGAAGT TTGCTGTGAT 
TGTGGGTCAC CGGACCCGAC ATGGCTTTCC ACAAACCTTG GGATTTTAAC CTGTATAGAA 
TGTTCGGGCA TACATAGGGA AATGGGGGTT CATATCTCTA GAATCCAATC TCTGGAATTA 
GACAAATTAG GGACTTCTGA ACTCTTGCTG GCCAAGAATG TAGGAAACAA TAGCTTCAAT 
GATATTATGG AAGGGAATTT GCTGTCTCCG TCAGCCAAGC CTTCTCCCTC CAGTGATATG 
ATTGCACGTA AAGAATATAT CACTGCCAAG TACGTGGAGC GGAGGTTTTC CAGAAAGATC 
TGTTCTACTG GGGCAGAAAA ACTGAATGAG CTACTTGAGG CTGTGAAATC CAGGGACTTA 
CTTGCACTAA TCCAAGTCTA TGCAGAAGGT GTAGAGATCA TGGAACCACT GCTAGAATCT 
GGACAGGAGC CAGGTGAAAC TGCACTTCAT TATGCAGTCC GGACAGCTGA CCACACGTCT 
CTTCCCTTGG TGGACTTCCT GGTACAAAAC TGTGGAAATT TAGATAAGCA GACTGGAAAG 
GGCAACACTG CTTTACACTA CTGCTGCTTA TATAACAAGC CGGAATGCTT AAAATTGCTT 
TTGAGGGGAA AGCCAACTAT TGATGCTGTT AACCATATTG GTGAGACGGC TTTTGATGTA 
GCAAAGAGAC AGAAAGCAGC CCAGTGTGAG GAACTGGTAT CCCAAGCCCT GGCAGGGAAG 
CTGAATCCTC ATGTTCACGT GGAGTACGAC TGGAACCTGC GTCAGGAAGA AATGGATGAG 
AGCGACGATG ATTTGGAAGA TAAGCCAAGC CCAATAAAGA AAGAGCGTTC CCCCAGACCC 
CAGAGCTTCT GCCATTCGTC CAGCATTTCC CCACAAGACA AGCTGTCGCT GCCAGGTTTC 
AATACTCCTC GGGACAAGCA GAGACTCTCT TATGGCGCCT TTACCAACCA AATATTCATG 
TCTACAAGCA CAGATTCCCC CACGTCTCCT ACTGCAGATG CCCCTCCGCT GCCCCCTAGG 
AACGCAGGCA AAGTGGCGAG TGGCCCTACT TCAACCCTCC CTCTAAGCAC CCAATCCCCT 
AGTGGCAGTT CCACTCTGTC CAAGAAGAGA CCTCCTCCCC CACCCCCGGG ACACAAACGA 
ACCCTCTCCG ACCCACCAAG TCCGCTACCT CATGGCCCCC CCAGTAAGGG CTCAGTTGTT 
TGTGGTAATG ACTTGGGCCC GAGTGCTGCA GACAGAACTC TAAATAAGTT TGAGAGATTG 
TCACAGTCAT CAGGCACAGG AACAACAAAG ACTGCATTAG GCCCAAGAGT CCTACCAAAA 
CTACCTCAGA AAGTGGCACT TAGGAAAATA GACACCACTC ATCATCTCTC AATGGATAAG 
TATAACCAGC AACCCGAAAT CTTCCAGAAA TCTCCCCAGC CTGGAGATCT GCCTCAGAAG 
CCACAAATTG CAGATCTTCC TCCGAAACCG CAGCCTTTGG ATATCGCCCA GAAACCACAC 
GTCGGAGATC TACCTCCCAA GCCCGGAGAA TTGCCTCCTA AACCTCAGCT AGGAGATTTG 
CCCCCGAAAC CGCAAGATTT ACCTCCAAAG CCACAGCTGA AAGATCTTCC GCCCAAGCCC 
AACCTGGCAG AGGTGATGTG CAAAACTCCT GTTGCGGATA TAGTCCCCAA ACCTCATACC 
AATGAGGCCA ATCAAAATTC CCAGCCGGTG GATGCGACCC ATAATGCACA GCCCACGGAC 
CAAACCCCTG TCCAGAAGCA AGTTCCAGAA ACTTCCAATA ATGGCCCACC GCCTCTTCCA 
GAAACACCCG TGCCACTTCC CCGAAAGATA AACACAGGGA AGAATAAGCT TAAACGAGTT 
AAAACAATAT ACGACTGCCA AGCGGACAAT GATGATGAAC TCACATTTGC GGAGGGGGAA 
GTGATTATCG TCACTGGAGA GGAGGACCAG GAGTGGTGGA TTGGGCACAT CGAAGGCTTC 
CCGGACAGGA AAGGAGTATT CCCTGTGTCC TTCGTCCACC TGTTGACTGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819587.1
CDS	498..3851
Translation	MMPDQISVSEFISETTEDYNSPTTSSFTTRLQSCRNSINVLEEALDQDRTALQKVKKSVKAIYNSGQDHVQNEENYAQALDKFGSNFLSRDNADLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGVKGDLKKPFDRAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDQKESRRDSQSKQAGYSMHQLQGNKEYGSEKKGYLLKKSDGIRKVWQRRKCTVKNGILTISHATSNRPPTKLNLLTCQVKPNADEKKCFDLISHNRTYHFQAEDEQEYIAWISVLTNSKEDALNMAFRGDQSTGESSLEDLTKAIIDDVQKTPGNEVCCDCGSPDPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDKLGTSELLLAKNVGNNSFNDIMEGNLLSPSAKPSPSSDMIARKEYITAKYVERRFSRKICSTGAEKLNELLEAVKSRDLLALIQVYAEGVEIMEPLLESGQEPGETALHYAVRTADHTSLPLVDFLVQNCGNLDKQTGKGNTALHYCCLYNKPECLKLLLRGKPTIDAVNHIGETAFDVAKRQKAAQCEELVSQALAGKLNPHVHVEYDWNLRQEEMDESDDDLEDKPSPIKKERSPRPQSFCHSSSISPQDKLSLPGFNTPRDKQRLSYGAFTNQIFMSTSTDSPTSPTADAPPLPPRNAGKVASGPTSTLPLSTQSPSGSSTLSKKRPPPPPPGHKRTLSDPPSPLPHGPPSKGSVVCGNDLGPSAADRTLNKFERLSQSSGTGTTKTALGPRVLPKLPQKVALRKIDTTHHLSMDKYNQQPEIFQKSPQPGDLPQKPQIADLPPKPQPLDIAQKPHVGDLPPKPGELPPKPQLGDLPPKPQDLPPKPQLKDLPPKPNLAEVMCKTPVADIVPKPHTNEANQNSQPVDATHNAQPTDQTPVQKQVPETSNNGPPPLPETPVPLPRKINTGKNKLKRVKTIYDCQADNDDELTFAEGEVIIVTGEEDQEWWIGHIEGFPDRKGVFPVSFVHLLTE

Target ORF information:

RefSeq Version	XM_012964133.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (asap1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012964133.2

ORF Insert Sequence:

ATGATGCCCG ATCAGATCTC AGTATCGGAG TTTATATCGG AGACCACAGA GGATTACAAT 
TCGCCAACCA CGTCCAGTTT CACGACCCGT CTTCAGAGCT GCAGGAATTC GATCAATGTG 
CTGGAAGAGG CCTTAGATCA AGATAGGACA GCGTTGCAGA AAGTGAAAAA ATCGGTGAAA 
GCAATTTATA ATTCAGGCCA AGATCATGTC CAAAATGAAG AAAACTATGC ACAAGCCTTA 
GATAAATTTG GAAGTAATTT TCTAAGTCGG GATAACGCAG ATTTGGGCAC CGCCTTCGTG 
AAATTCTCCA CTCTAACAAA AGAACTATCA ACGCTTCTTA AAAACCTGCT ACAGGGATTA 
AGCCACAATG TCATCTTCAC CTTGGATTCT CTCTTGAAAG GGGACTTAAA GGGCGTAAAA 
GGGGACCTTA AGAAGCCATT CGACAGAGCG TGGAAGGATT ATGAAACCAA ATTCACGAAA 
ATCGAAAAGG AGAAAAGGGA ACACGCCAAG CAACATGGGA TGATCCGTAC GGAGATTACT 
GGGGCCGAGA TTGCCGAAGA GATGGAGAAG GAGAGGAGAT TATTCCAGCT ACAGATGTGT 
GAGTATCTCA TTAAAGTTAA TGAAATCAAG ACTAAGAAAG GCGTGGATCT GCTGCAGAAC 
CTTATAAAGT ACTATCACGC ACAGTGCAAT TTCTTCCAGG ATGGATTGAA AACAGCTGAC 
AAGCTGAAGC AGTACATAGA GAAATTGGCT GCAGATTTGT ACAATATTAA GCAGACCCAA 
GATGAGGAGA AGAAACAGCT CACTGCCCTG CGAGACCTGA TAAAATCCTC TTTACAGCTG 
GATCAGAAGG AGTCTAGAAG AGATTCCCAG AGTAAGCAGG CAGGCTACAG CATGCACCAG 
CTCCAGGGGA ACAAGGAGTA TGGCAGCGAG AAGAAAGGCT ACCTGCTGAA AAAAAGTGAT 
GGCATCCGGA AAGTATGGCA GAGGAGGAAG TGCACTGTGA AAAATGGAAT CCTGACCATT 
TCCCATGCAA CGTCAAACAG GCCACCGACG AAGCTGAACC TGCTGACCTG CCAAGTCAAG 
CCGAACGCTG ATGAGAAGAA ATGCTTTGAT CTCATCTCAC ACAACAGAAC ATACCACTTC 
CAAGCAGAGG ACGAACAAGA ATACATTGCG TGGATATCAG TTCTGACAAA TAGCAAAGAA 
GATGCACTCA ATATGGCTTT CCGGGGAGAC CAGAGCACTG GCGAGAGCAG CCTGGAGGAT 
CTGACTAAGG CCATTATAGA CGATGTACAG AAAACGCCTG GAAATGAAGT TTGCTGTGAT 
TGTGGGTCAC CGGACCCGAC ATGGCTTTCC ACAAACCTTG GGATTTTAAC CTGTATAGAA 
TGTTCGGGCA TACATAGGGA AATGGGGGTT CATATCTCTA GAATCCAATC TCTGGAATTA 
GACAAATTAG GGACTTCTGA ACTCTTGCTG GCCAAGAATG TAGGAAACAA TAGCTTCAAT 
GATATTATGG AAGGGAATTT GCTGTCTCCG TCAGCCAAGC CTTCTCCCTC CAGTGATATG 
ATTGCACGTA AAGAATATAT CACTGCCAAG TACGTGGAGC GGAGGTTTTC CAGAAAGATC 
TGTTCTACTG GGGCAGAAAA ACTGAATGAG CTACTTGAGG CTGTGAAATC CAGGGACTTA 
CTTGCACTAA TCCAAGTCTA TGCAGAAGGT GTAGAGATCA TGGAACCACT GCTAGAATCT 
GGACAGGAGC CAGGTGAAAC TGCACTTCAT TATGCAGTCC GGACAGCTGA CCACACGTCT 
CTTCCCTTGG TGGACTTCCT GGTACAAAAC TGTGGAAATT TAGATAAGCA GACTGGAAAG 
GGCAACACTG CTTTACACTA CTGCTGCTTA TATAACAAGC CGGAATGCTT AAAATTGCTT 
TTGAGGGGAA AGCCAACTAT TGATGCTGTT AACCATATTG GTGAGACGGC TTTTGATGTA 
GCAAAGAGAC AGAAAGCAGC CCAGTGTGAG GAACTGGTAT CCCAAGCCCT GGCAGGGAAG 
CTGAATCCTC ATGTTCACGT GGAGTACGAC TGGAACCTGC GTCAGGAAGA AATGGATGAG 
AGCGACGATG ATTTGGAAGA TAAGCCAAGC CCAATAAAGA AAGAGCGTTC CCCCAGACCC 
CAGAGCTTCT GCCATTCGTC CAGCATTTCC CCACAAGACA AGCTGTCGCT GCCAGGTTTC 
AATACTCCTC GGGACAAGCA GAGACTCTCT TATGGCGCCT TTACCAACCA AATATTCATG 
TCTACAAGCA CAGATTCCCC CACGTCTCCT ACTGCAGATG CCCCTCCGCT GCCCCCTAGG 
AACGCAGGCA AAGTGGCGAG TGGCCCTACT TCAACCCTCC CTCTAAGCAC CCAATCCCCT 
AGTGGCAGTT CCACTCTGTC CAAGAAGAGA CCTCCTCCCC CACCCCCGGG ACACAAACGA 
ACCCTCTCCG ACCCACCAAG TCCGCTACCT CATGGCCCCC CCAGTAAGGG CTCAGTTGTT 
TGTGGTAATG ACTTGGGCCC GAGTGCTGCA GACAGAACTC TAAATAAGTT TGAGAGATTG 
TCACAGTCAT CAGGCACAGG AACAACAAAG ACTGCATTAG GCCCAAGAGT CCTACCAAAA 
CTACCTCAGA AAGTGGCACT TAGGAAAATA GACACCACTC ATCATCTCTC AATGGATAAG 
TATAACCAGC AACCCGAAAT CTTCCAGAAA TCTCCCCAGC CTGGAGATCT GCCTCAGAAG 
CCACAAATTG CAGATCTTCC TCCGAAACCG CAGCCTTTGG ATATCGCCCA GAAACCACAC 
GTCGGAGATC TACCTCCCAA GCCCGGAGAA TTGCCTCCTA AACCTCAGCT AGGAGATTTG 
CCCCCGAAAC CGCAAGATTT ACCTCCAAAG CCACAGCTGA AAGATCTTCC GCCCAAGCCC 
AACCTGGCAG AGGTGATGTG CAAAACTCCT GTTGCGGATA TAGTCCCCAA ACCTCATACC 
AATGAGGCCA ATCAAAATTC CCAGCCGGTG GATGCGACCC ATAATGCACA GCCCACGGAC 
CAAACCCCTG TCCAGAAGCA AGTTCCAGAA ACTTCCAATA ATGGCCCACC GCCTCTTCCA 
GAAACACCCG TGCCACTTCC CCGAAAGATA AACACAGGGA AGAATAAGCT TAAACGAGTT 
AAAACAATAT ACGACTGCCA AGCGGACAAT GATGATGAAC TCACATTTGC GGAGGGGGAA 
GTGATTATCG TCACTGGAGA GGAGGACCAG GAGTGGTGGA TTGGGCACAT CGAAGGCTTC 
CCGGACAGGA AAGGAGTATT CCCTGTGTCC TTCGTCCACC TGTTGACTGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

asap1 ( XM_012964134.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819588.1 Xenopus tropicalis ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (asap1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa29915
Clone ID Related Accession (Same CDS sequence)	XM_012964134.2
Accession Version	XM_012964134.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012964134.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGATC AGATCTCAGT ATCGGAGTTT ATATCGGAGA CCACAGAGGA TTACAATTCG 
CCAACCACGT CCAGTTTCAC GACCCGTCTT CAGAGCTGCA GGAATTCGAT CAATGTGCTG 
GAAGAGGCCT TAGATCAAGA TAGGACAGCG TTGCAGAAAG TGAAAAAATC GGTGAAAGCA 
ATTTATAATT CAGGCCAAGA TCATGTCCAA AATGAAGAAA ACTATGCACA AGCCTTAGAT 
AAATTTGGAA GTAATTTTCT AAGTCGGGAT AACGCAGATT TGGGCACCGC CTTCGTGAAA 
TTCTCCACTC TAACAAAAGA ACTATCAACG CTTCTTAAAA ACCTGCTACA GGGATTAAGC 
CACAATGTCA TCTTCACCTT GGATTCTCTC TTGAAAGGGG ACTTAAAGGG CGTAAAAGGG 
GACCTTAAGA AGCCATTCGA CAGAGCGTGG AAGGATTATG AAACCAAATT CACGAAAATC 
GAAAAGGAGA AAAGGGAACA CGCCAAGCAA CATGGGATGA TCCGTACGGA GATTACTGGG 
GCCGAGATTG CCGAAGAGAT GGAGAAGGAG AGGAGATTAT TCCAGCTACA GATGTGTGAG 
TATCTCATTA AAGTTAATGA AATCAAGACT AAGAAAGGCG TGGATCTGCT GCAGAACCTT 
ATAAAGTACT ATCACGCACA GTGCAATTTC TTCCAGGATG GATTGAAAAC AGCTGACAAG 
CTGAAGCAGT ACATAGAGAA ATTGGCTGCA GATTTGTACA ATATTAAGCA GACCCAAGAT 
GAGGAGAAGA AACAGCTCAC TGCCCTGCGA GACCTGATAA AATCCTCTTT ACAGCTGGAT 
CAGAAGGAGT CTAGAAGAGA TTCCCAGAGT AAGCAGGCAG GCTACAGCAT GCACCAGCTC 
CAGGGGAACA AGGAGTATGG CAGCGAGAAG AAAGGCTACC TGCTGAAAAA AAGTGATGGC 
ATCCGGAAAG TATGGCAGAG GAGGAAGTGC ACTGTGAAAA ATGGAATCCT GACCATTTCC 
CATGCAACGT CAAACAGGCC ACCGACGAAG CTGAACCTGC TGACCTGCCA AGTCAAGCCG 
AACGCTGATG AGAAGAAATG CTTTGATCTC ATCTCACACA ACAGAACATA CCACTTCCAA 
GCAGAGGACG AACAAGAATA CATTGCGTGG ATATCAGTTC TGACAAATAG CAAAGAAGAT 
GCACTCAATA TGGCTTTCCG GGGAGACCAG AGCACTGGCG AGAGCAGCCT GGAGGATCTG 
ACTAAGGCCA TTATAGACGA TGTACAGAAA ACGCCTGGAA ATGAAGTTTG CTGTGATTGT 
GGGTCACCGG ACCCGACATG GCTTTCCACA AACCTTGGGA TTTTAACCTG TATAGAATGT 
TCGGGCATAC ATAGGGAAAT GGGGGTTCAT ATCTCTAGAA TCCAATCTCT GGAATTAGAC 
AAATTAGGGA CTTCTGAACT CTTGCTGGCC AAGAATGTAG GAAACAATAG CTTCAATGAT 
ATTATGGAAG GGAATTTGCT GTCTCCGTCA GCCAAGCCTT CTCCCTCCAG TGATATGATT 
GCACGTAAAG AATATATCAC TGCCAAGTAC GTGGAGCGGA GGTTTTCCAG AAAGATCTGT 
TCTACTGGGG CAGAAAAACT GAATGAGCTA CTTGAGGCTG TGAAATCCAG GGACTTACTT 
GCACTAATCC AAGTCTATGC AGAAGGTGTA GAGATCATGG AACCACTGCT AGAATCTGGA 
CAGGAGCCAG GTGAAACTGC ACTTCATTAT GCAGTCCGGA CAGCTGACCA CACGTCTCTT 
CCCTTGGTGG ACTTCCTGGT ACAAAACTGT GGAAATTTAG ATAAGCAGAC TGGAAAGGGC 
AACACTGCTT TACACTACTG CTGCTTATAT AACAAGCCGG AATGCTTAAA ATTGCTTTTG 
AGGGGAAAGC CAACTATTGA TGCTGTTAAC CATATTGGTG AGACGGCTTT TGATGTAGCA 
AAGAGACAGA AAGCAGCCCA GTGTGAGGAA CTGGTATCCC AAGCCCTGGC AGGGAAGCTG 
AATCCTCATG TTCACGTGGA GTACGACTGG AACCTGCGTC AGGAAGAAAT GGATGAGAGC 
GACGATGATT TGGAAGATAA GCCAAGCCCA ATAAAGAAAG AGCGTTCCCC CAGACCCCAG 
AGCTTCTGCC ATTCGTCCAG CATTTCCCCA CAAGACAAGC TGTCGCTGCC AGGTTTCAAT 
ACTCCTCGGG ACAAGCAGAG ACTCTCTTAT GGCGCCTTTA CCAACCAAAT ATTCATGTCT 
ACAAGCACAG ATTCCCCCAC GTCTCCTACT GCAGATGCCC CTCCGCTGCC CCCTAGGAAC 
GCAGGCAAAG TGGCGAGTGG CCCTACTTCA ACCCTCCCTC TAAGCACCCA ATCCCCTAGT 
GGCAGTTCCA CTCTGTCCAA GAAGAGACCT CCTCCCCCAC CCCCGGGACA CAAACGAACC 
CTCTCCGACC CACCAAGTCC GCTACCTCAT GGCCCCCCCA GTAAGGGCTC AGTTGTTTGT 
GGTAATGACT TGGGCCCGAG TGCTGCAGAC AGAACTCTAA ATAAGTTTGA GAGATTGTCA 
CAGTCATCAG GCACAGGAAC AACAAAGACT GCATTAGGCC CAAGAGTCCT ACCAAAACTA 
CCTCAGAAAG TGGCACTTAG GAAAATAGAC ACCACTCATC ATCTCTCAAT GGATAAGTAT 
AACCAGCAAC CCGAAATCTT CCAGAAATCT CCCCAGCCTG GAGATCTGCC TCAGAAGCCA 
CAAATTGCAG ATCTTCCTCC GAAACCGCAG CCTTTGGATA TCGCCCAGAA ACCACACGTC 
GGAGATCTAC CTCCCAAGCC CGGAGAATTG CCTCCTAAAC CTCAGCTAGG AGATTTGCCC 
CCGAAACCGC AAGATTTACC TCCAAAGCCA CAGCTGAAAG ATCTTCCGCC CAAGCCCAAC 
CTGGCAGAGG TGATGTGCAA AACTCCTGTT GCGGATATAG TCCCCAAACC TCATACCAAT 
GAGGCCAATC AAAATTCCCA GCCGGTGGAT GCGACCCATA ATGCACAGCC CACGGACCAA 
ACCCCTGTCC AGAAGCAAGT TCCAGAAACT TCCAATAATG GCCCACCGCC TCTTCCAGAA 
ACACCCGTGC CACTTCCCCG AAAGATAAAC ACAGGGAAGA ATAAGCTTAA ACGAGTTAAA 
ACAATATACG ACTGCCAAGC GGACAATGAT GATGAACTCA CATTTGCGGA GGGGGAAGTG 
ATTATCGTCA CTGGAGAGGA GGACCAGGAG TGGTGGATTG GGCACATCGA AGGCTTCCCG 
GACAGGAAAG GAGTATTCCC TGTGTCCTTC GTCCACCTGT TGACTGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819588.1
CDS	305..3655
Translation	MPDQISVSEFISETTEDYNSPTTSSFTTRLQSCRNSINVLEEALDQDRTALQKVKKSVKAIYNSGQDHVQNEENYAQALDKFGSNFLSRDNADLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGVKGDLKKPFDRAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDQKESRRDSQSKQAGYSMHQLQGNKEYGSEKKGYLLKKSDGIRKVWQRRKCTVKNGILTISHATSNRPPTKLNLLTCQVKPNADEKKCFDLISHNRTYHFQAEDEQEYIAWISVLTNSKEDALNMAFRGDQSTGESSLEDLTKAIIDDVQKTPGNEVCCDCGSPDPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDKLGTSELLLAKNVGNNSFNDIMEGNLLSPSAKPSPSSDMIARKEYITAKYVERRFSRKICSTGAEKLNELLEAVKSRDLLALIQVYAEGVEIMEPLLESGQEPGETALHYAVRTADHTSLPLVDFLVQNCGNLDKQTGKGNTALHYCCLYNKPECLKLLLRGKPTIDAVNHIGETAFDVAKRQKAAQCEELVSQALAGKLNPHVHVEYDWNLRQEEMDESDDDLEDKPSPIKKERSPRPQSFCHSSSISPQDKLSLPGFNTPRDKQRLSYGAFTNQIFMSTSTDSPTSPTADAPPLPPRNAGKVASGPTSTLPLSTQSPSGSSTLSKKRPPPPPPGHKRTLSDPPSPLPHGPPSKGSVVCGNDLGPSAADRTLNKFERLSQSSGTGTTKTALGPRVLPKLPQKVALRKIDTTHHLSMDKYNQQPEIFQKSPQPGDLPQKPQIADLPPKPQPLDIAQKPHVGDLPPKPGELPPKPQLGDLPPKPQDLPPKPQLKDLPPKPNLAEVMCKTPVADIVPKPHTNEANQNSQPVDATHNAQPTDQTPVQKQVPETSNNGPPPLPETPVPLPRKINTGKNKLKRVKTIYDCQADNDDELTFAEGEVIIVTGEEDQEWWIGHIEGFPDRKGVFPVSFVHLLTE

Target ORF information:

RefSeq Version	XM_012964134.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (asap1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012964134.2

ORF Insert Sequence:

ATGCCCGATC AGATCTCAGT ATCGGAGTTT ATATCGGAGA CCACAGAGGA TTACAATTCG 
CCAACCACGT CCAGTTTCAC GACCCGTCTT CAGAGCTGCA GGAATTCGAT CAATGTGCTG 
GAAGAGGCCT TAGATCAAGA TAGGACAGCG TTGCAGAAAG TGAAAAAATC GGTGAAAGCA 
ATTTATAATT CAGGCCAAGA TCATGTCCAA AATGAAGAAA ACTATGCACA AGCCTTAGAT 
AAATTTGGAA GTAATTTTCT AAGTCGGGAT AACGCAGATT TGGGCACCGC CTTCGTGAAA 
TTCTCCACTC TAACAAAAGA ACTATCAACG CTTCTTAAAA ACCTGCTACA GGGATTAAGC 
CACAATGTCA TCTTCACCTT GGATTCTCTC TTGAAAGGGG ACTTAAAGGG CGTAAAAGGG 
GACCTTAAGA AGCCATTCGA CAGAGCGTGG AAGGATTATG AAACCAAATT CACGAAAATC 
GAAAAGGAGA AAAGGGAACA CGCCAAGCAA CATGGGATGA TCCGTACGGA GATTACTGGG 
GCCGAGATTG CCGAAGAGAT GGAGAAGGAG AGGAGATTAT TCCAGCTACA GATGTGTGAG 
TATCTCATTA AAGTTAATGA AATCAAGACT AAGAAAGGCG TGGATCTGCT GCAGAACCTT 
ATAAAGTACT ATCACGCACA GTGCAATTTC TTCCAGGATG GATTGAAAAC AGCTGACAAG 
CTGAAGCAGT ACATAGAGAA ATTGGCTGCA GATTTGTACA ATATTAAGCA GACCCAAGAT 
GAGGAGAAGA AACAGCTCAC TGCCCTGCGA GACCTGATAA AATCCTCTTT ACAGCTGGAT 
CAGAAGGAGT CTAGAAGAGA TTCCCAGAGT AAGCAGGCAG GCTACAGCAT GCACCAGCTC 
CAGGGGAACA AGGAGTATGG CAGCGAGAAG AAAGGCTACC TGCTGAAAAA AAGTGATGGC 
ATCCGGAAAG TATGGCAGAG GAGGAAGTGC ACTGTGAAAA ATGGAATCCT GACCATTTCC 
CATGCAACGT CAAACAGGCC ACCGACGAAG CTGAACCTGC TGACCTGCCA AGTCAAGCCG 
AACGCTGATG AGAAGAAATG CTTTGATCTC ATCTCACACA ACAGAACATA CCACTTCCAA 
GCAGAGGACG AACAAGAATA CATTGCGTGG ATATCAGTTC TGACAAATAG CAAAGAAGAT 
GCACTCAATA TGGCTTTCCG GGGAGACCAG AGCACTGGCG AGAGCAGCCT GGAGGATCTG 
ACTAAGGCCA TTATAGACGA TGTACAGAAA ACGCCTGGAA ATGAAGTTTG CTGTGATTGT 
GGGTCACCGG ACCCGACATG GCTTTCCACA AACCTTGGGA TTTTAACCTG TATAGAATGT 
TCGGGCATAC ATAGGGAAAT GGGGGTTCAT ATCTCTAGAA TCCAATCTCT GGAATTAGAC 
AAATTAGGGA CTTCTGAACT CTTGCTGGCC AAGAATGTAG GAAACAATAG CTTCAATGAT 
ATTATGGAAG GGAATTTGCT GTCTCCGTCA GCCAAGCCTT CTCCCTCCAG TGATATGATT 
GCACGTAAAG AATATATCAC TGCCAAGTAC GTGGAGCGGA GGTTTTCCAG AAAGATCTGT 
TCTACTGGGG CAGAAAAACT GAATGAGCTA CTTGAGGCTG TGAAATCCAG GGACTTACTT 
GCACTAATCC AAGTCTATGC AGAAGGTGTA GAGATCATGG AACCACTGCT AGAATCTGGA 
CAGGAGCCAG GTGAAACTGC ACTTCATTAT GCAGTCCGGA CAGCTGACCA CACGTCTCTT 
CCCTTGGTGG ACTTCCTGGT ACAAAACTGT GGAAATTTAG ATAAGCAGAC TGGAAAGGGC 
AACACTGCTT TACACTACTG CTGCTTATAT AACAAGCCGG AATGCTTAAA ATTGCTTTTG 
AGGGGAAAGC CAACTATTGA TGCTGTTAAC CATATTGGTG AGACGGCTTT TGATGTAGCA 
AAGAGACAGA AAGCAGCCCA GTGTGAGGAA CTGGTATCCC AAGCCCTGGC AGGGAAGCTG 
AATCCTCATG TTCACGTGGA GTACGACTGG AACCTGCGTC AGGAAGAAAT GGATGAGAGC 
GACGATGATT TGGAAGATAA GCCAAGCCCA ATAAAGAAAG AGCGTTCCCC CAGACCCCAG 
AGCTTCTGCC ATTCGTCCAG CATTTCCCCA CAAGACAAGC TGTCGCTGCC AGGTTTCAAT 
ACTCCTCGGG ACAAGCAGAG ACTCTCTTAT GGCGCCTTTA CCAACCAAAT ATTCATGTCT 
ACAAGCACAG ATTCCCCCAC GTCTCCTACT GCAGATGCCC CTCCGCTGCC CCCTAGGAAC 
GCAGGCAAAG TGGCGAGTGG CCCTACTTCA ACCCTCCCTC TAAGCACCCA ATCCCCTAGT 
GGCAGTTCCA CTCTGTCCAA GAAGAGACCT CCTCCCCCAC CCCCGGGACA CAAACGAACC 
CTCTCCGACC CACCAAGTCC GCTACCTCAT GGCCCCCCCA GTAAGGGCTC AGTTGTTTGT 
GGTAATGACT TGGGCCCGAG TGCTGCAGAC AGAACTCTAA ATAAGTTTGA GAGATTGTCA 
CAGTCATCAG GCACAGGAAC AACAAAGACT GCATTAGGCC CAAGAGTCCT ACCAAAACTA 
CCTCAGAAAG TGGCACTTAG GAAAATAGAC ACCACTCATC ATCTCTCAAT GGATAAGTAT 
AACCAGCAAC CCGAAATCTT CCAGAAATCT CCCCAGCCTG GAGATCTGCC TCAGAAGCCA 
CAAATTGCAG ATCTTCCTCC GAAACCGCAG CCTTTGGATA TCGCCCAGAA ACCACACGTC 
GGAGATCTAC CTCCCAAGCC CGGAGAATTG CCTCCTAAAC CTCAGCTAGG AGATTTGCCC 
CCGAAACCGC AAGATTTACC TCCAAAGCCA CAGCTGAAAG ATCTTCCGCC CAAGCCCAAC 
CTGGCAGAGG TGATGTGCAA AACTCCTGTT GCGGATATAG TCCCCAAACC TCATACCAAT 
GAGGCCAATC AAAATTCCCA GCCGGTGGAT GCGACCCATA ATGCACAGCC CACGGACCAA 
ACCCCTGTCC AGAAGCAAGT TCCAGAAACT TCCAATAATG GCCCACCGCC TCTTCCAGAA 
ACACCCGTGC CACTTCCCCG AAAGATAAAC ACAGGGAAGA ATAAGCTTAA ACGAGTTAAA 
ACAATATACG ACTGCCAAGC GGACAATGAT GATGAACTCA CATTTGCGGA GGGGGAAGTG 
ATTATCGTCA CTGGAGAGGA GGACCAGGAG TGGTGGATTG GGCACATCGA AGGCTTCCCG 
GACAGGAAAG GAGTATTCCC TGTGTCCTTC GTCCACCTGT TGACTGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

asap1 ( XM_012964135.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819589.1 Xenopus tropicalis ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (asap1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXa29916
Clone ID Related Accession (Same CDS sequence)	XM_012964135.2
Accession Version	XM_012964135.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3240bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012964135.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGATCTT CGGCCTCTAG ACTTTCGAGT TTTTCCTCTA GAGACTCACT ATGGAACCGG 
ATGCCCGATC AGATCTCAGT ATCGGAGTTT ATATCGGAGA CCACAGAGGA TTACAATTCG 
CCAACCACGT CCAGTTTCAC GACCCGTCTT CAGAGCTGCA GGAATTCGAT CAATGTGCTG 
GAAGAGGCCT TAGATCAAGA TAGGACAGCG TTGCAGAAAG TGAAAAAATC GGTGAAAGCA 
ATTTATAATT CAGGCCAAGA TCATGTCCAA AATGAAGAAA ACTATGCACA AGCCTTAGAT 
AAATTTGGAA GTAATTTTCT AAGTCGGGAT AACGCAGATT TGGGCACCGC CTTCGTGAAA 
TTCTCCACTC TAACAAAAGA ACTATCAACG CTTCTTAAAA ACCTGCTACA GGGATTAAGC 
CACAATGTCA TCTTCACCTT GGATTCTCTC TTGAAAGGGG ACTTAAAGGG CGTAAAAGGG 
GACCTTAAGA AGCCATTCGA CAGAGCGTGG AAGGATTATG AAACCAAATT CACGAAAATC 
GAAAAGGAGA AAAGGGAACA CGCCAAGCAA CATGGGATGA TCCGTACGGA GATTACTGGG 
GCCGAGATTG CCGAAGAGAT GGAGAAGGAG AGGAGATTAT TCCAGCTACA GATGTGTGAG 
TATCTCATTA AAGTTAATGA AATCAAGACT AAGAAAGGCG TGGATCTGCT GCAGAACCTT 
ATAAAGTACT ATCACGCACA GTGCAATTTC TTCCAGGATG GATTGAAAAC AGCTGACAAG 
CTGAAGCAGT ACATAGAGAA ATTGGCTGCA GATTTGTACA ATATTAAGCA GACCCAAGAT 
GAGGAGAAGA AACAGCTCAC TGCCCTGCGA GACCTGATAA AATCCTCTTT ACAGCTGGAT 
CAGAAGGAGT CTAGAAGAGA TTCCCAGAGT AAGCAGGCAG GCTACAGCAT GCACCAGCTC 
CAGGGGAACA AGGAGTATGG CAGCGAGAAG AAAGGCTACC TGCTGAAAAA AAGTGATGGC 
ATCCGGAAAG TATGGCAGAG GAGGAAGTGC ACTGTGAAAA ATGGAATCCT GACCATTTCC 
CATGCAACGT CAAACAGGCC ACCGACGAAG CTGAACCTGC TGACCTGCCA AGTCAAGCCG 
AACGCTGATG AGAAGAAATG CTTTGATCTC ATCTCACACA ACAGAACATA CCACTTCCAA 
GCAGAGGACG AACAAGAATA CATTGCGTGG ATATCAGTTC TGACAAATAG CAAAGAAGAT 
GCACTCAATA TGGCTTTCCG GGGAGACCAG AGCACTGGCG AGAGCAGCCT GGAGGATCTG 
ACTAAGGCCA TTATAGACGA TGTACAGAAA ACGCCTGGAA ATGAAGTTTG CTGTGATTGT 
GGGTCACCGG ACCCGACATG GCTTTCCACA AACCTTGGGA TTTTAACCTG TATAGAATGT 
TCGGGCATAC ATAGGGAAAT GGGGGTTCAT ATCTCTAGAA TCCAATCTCT GGAATTAGAC 
AAATTAGGGA CTTCTGAACT CTTGCTGGCC AAGAATGTAG GAAACAATAG CTTCAATGAT 
ATTATGGAAG GGAATTTGCT GTCTCCGTCA GCCAAGCCTT CTCCCTCCAG TGATATGATT 
GCACGTAAAG AATATATCAC TGCCAAGTAC GTGGAGCGGA GGTTTTCCAG AAAGATCTGT 
TCTACTGGGG CAGAAAAACT GAATGAGCTA CTTGAGGCTG TGAAATCCAG GGACTTACTT 
GCACTAATCC AAGTCTATGC AGAAGGTGTA GAGATCATGG AACCACTGCT AGAATCTGGA 
CAGGAGCCAG GTGAAACTGC ACTTCATTAT GCAGTCCGGA CAGCTGACCA CACGTCTCTT 
CCCTTGGTGG ACTTCCTGGT ACAAAACTGT GGAAATTTAG ATAAGCAGAC TGGAAAGGGC 
AACACTGCTT TACACTACTG CTGCTTATAT AACAAGCCGG AATGCTTAAA ATTGCTTTTG 
AGGGGAAAGC CAACTATTGA TGCTGTTAAC CATATTGGTG AGACGGCTTT TGATGTAGCA 
AAGAGACAGA AAGCAGCCCA GTGTGAGGAA CTGGTATCCC AAGCCCTGGC AGGGAAGCTG 
AATCCTCATG TTCACGTGGA GTACGACTGG AACCTGCGTC AGGAAGAAAT GGATGAGAGC 
GACGATGATT TGGAAGATAA GCCAAGCCCA ATAAAGAAAG AGCGTTCCCC CAGACCCCAG 
AGCTTCTGCC ATTCGTCCAG CATTTCCCCA CAAGACAAGC TGTCGCTGCC AGGTTTCAAT 
ACTCCTCGGG ACAAGCAGAG ACTCTCTTAT GGCGCCTTTA CCAACCAAAT ATTCATGTCT 
ACAAGCACAG ATTCCCCCAC GTCTCCTACT GCAGATGCCC CTCCGCTGCC CCCTAGGAAC 
GCAGGCAAAG GTAATGACTT GGGCCCGAGT GCTGCAGACA GAACTCTAAA TAAGTTTGAG 
AGATTGTCAC AGTCATCAGG CACAGGAACA ACAAAGACTG CATTAGGCCC AAGAGTCCTA 
CCAAAACTAC CTCAGAAAGT GGCACTTAGG AAAATAGACA CCACTCATCA TCTCTCAATG 
GATAAGTATA ACCAGCAACC CGAAATCTTC CAGAAATCTC CCCAGCCTGG AGATCTGCCT 
CAGAAGCCAC AAATTGCAGA TCTTCCTCCG AAACCGCAGC CTTTGGATAT CGCCCAGAAA 
CCACACGTCG GAGATCTACC TCCCAAGCCC GGAGAATTGC CTCCTAAACC TCAGCTAGGA 
GATTTGCCCC CGAAACCGCA AGATTTACCT CCAAAGCCAC AGCTGAAAGA TCTTCCGCCC 
AAGCCCAACC TGGCAGAGGT GATGTGCAAA ACTCCTGTTG CGGATATAGT CCCCAAACCT 
CATACCAATG AGGCCAATCA AAATTCCCAG CCGGTGGATG CGACCCATAA TGCACAGCCC 
ACGGACCAAA CCCCTGTCCA GAAGCAAGTT CCAGAAACTT CCAATAATGG CCCACCGCCT 
CTTCCAGAAA CACCCGTGCC ACTTCCCCGA AAGATAAACA CAGGGAAGAA TAAGCTTAAA 
CGAGTTAAAA CAATATACGA CTGCCAAGCG GACAATGATG ATGAACTCAC ATTTGCGGAG 
GGGGAAGTGA TTATCGTCAC TGGAGAGGAG GACCAGGAGT GGTGGATTGG GCACATCGAA 
GGCTTCCCGG ACAGGAAAGG AGTATTCCCT GTGTCCTTCG TCCACCTGTT GACTGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819589.1
CDS	30..3269
Translation	MRSSASRLSSFSSRDSLWNRMPDQISVSEFISETTEDYNSPTTSSFTTRLQSCRNSINVLEEALDQDRTALQKVKKSVKAIYNSGQDHVQNEENYAQALDKFGSNFLSRDNADLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGVKGDLKKPFDRAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDQKESRRDSQSKQAGYSMHQLQGNKEYGSEKKGYLLKKSDGIRKVWQRRKCTVKNGILTISHATSNRPPTKLNLLTCQVKPNADEKKCFDLISHNRTYHFQAEDEQEYIAWISVLTNSKEDALNMAFRGDQSTGESSLEDLTKAIIDDVQKTPGNEVCCDCGSPDPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDKLGTSELLLAKNVGNNSFNDIMEGNLLSPSAKPSPSSDMIARKEYITAKYVERRFSRKICSTGAEKLNELLEAVKSRDLLALIQVYAEGVEIMEPLLESGQEPGETALHYAVRTADHTSLPLVDFLVQNCGNLDKQTGKGNTALHYCCLYNKPECLKLLLRGKPTIDAVNHIGETAFDVAKRQKAAQCEELVSQALAGKLNPHVHVEYDWNLRQEEMDESDDDLEDKPSPIKKERSPRPQSFCHSSSISPQDKLSLPGFNTPRDKQRLSYGAFTNQIFMSTSTDSPTSPTADAPPLPPRNAGKGNDLGPSAADRTLNKFERLSQSSGTGTTKTALGPRVLPKLPQKVALRKIDTTHHLSMDKYNQQPEIFQKSPQPGDLPQKPQIADLPPKPQPLDIAQKPHVGDLPPKPGELPPKPQLGDLPPKPQDLPPKPQLKDLPPKPNLAEVMCKTPVADIVPKPHTNEANQNSQPVDATHNAQPTDQTPVQKQVPETSNNGPPPLPETPVPLPRKINTGKNKLKRVKTIYDCQADNDDELTFAEGEVIIVTGEEDQEWWIGHIEGFPDRKGVFPVSFVHLLTE

Target ORF information:

RefSeq Version	XM_012964135.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (asap1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012964135.2

ORF Insert Sequence:

ATGCGATCTT CGGCCTCTAG ACTTTCGAGT TTTTCCTCTA GAGACTCACT ATGGAACCGG 
ATGCCCGATC AGATCTCAGT ATCGGAGTTT ATATCGGAGA CCACAGAGGA TTACAATTCG 
CCAACCACGT CCAGTTTCAC GACCCGTCTT CAGAGCTGCA GGAATTCGAT CAATGTGCTG 
GAAGAGGCCT TAGATCAAGA TAGGACAGCG TTGCAGAAAG TGAAAAAATC GGTGAAAGCA 
ATTTATAATT CAGGCCAAGA TCATGTCCAA AATGAAGAAA ACTATGCACA AGCCTTAGAT 
AAATTTGGAA GTAATTTTCT AAGTCGGGAT AACGCAGATT TGGGCACCGC CTTCGTGAAA 
TTCTCCACTC TAACAAAAGA ACTATCAACG CTTCTTAAAA ACCTGCTACA GGGATTAAGC 
CACAATGTCA TCTTCACCTT GGATTCTCTC TTGAAAGGGG ACTTAAAGGG CGTAAAAGGG 
GACCTTAAGA AGCCATTCGA CAGAGCGTGG AAGGATTATG AAACCAAATT CACGAAAATC 
GAAAAGGAGA AAAGGGAACA CGCCAAGCAA CATGGGATGA TCCGTACGGA GATTACTGGG 
GCCGAGATTG CCGAAGAGAT GGAGAAGGAG AGGAGATTAT TCCAGCTACA GATGTGTGAG 
TATCTCATTA AAGTTAATGA AATCAAGACT AAGAAAGGCG TGGATCTGCT GCAGAACCTT 
ATAAAGTACT ATCACGCACA GTGCAATTTC TTCCAGGATG GATTGAAAAC AGCTGACAAG 
CTGAAGCAGT ACATAGAGAA ATTGGCTGCA GATTTGTACA ATATTAAGCA GACCCAAGAT 
GAGGAGAAGA AACAGCTCAC TGCCCTGCGA GACCTGATAA AATCCTCTTT ACAGCTGGAT 
CAGAAGGAGT CTAGAAGAGA TTCCCAGAGT AAGCAGGCAG GCTACAGCAT GCACCAGCTC 
CAGGGGAACA AGGAGTATGG CAGCGAGAAG AAAGGCTACC TGCTGAAAAA AAGTGATGGC 
ATCCGGAAAG TATGGCAGAG GAGGAAGTGC ACTGTGAAAA ATGGAATCCT GACCATTTCC 
CATGCAACGT CAAACAGGCC ACCGACGAAG CTGAACCTGC TGACCTGCCA AGTCAAGCCG 
AACGCTGATG AGAAGAAATG CTTTGATCTC ATCTCACACA ACAGAACATA CCACTTCCAA 
GCAGAGGACG AACAAGAATA CATTGCGTGG ATATCAGTTC TGACAAATAG CAAAGAAGAT 
GCACTCAATA TGGCTTTCCG GGGAGACCAG AGCACTGGCG AGAGCAGCCT GGAGGATCTG 
ACTAAGGCCA TTATAGACGA TGTACAGAAA ACGCCTGGAA ATGAAGTTTG CTGTGATTGT 
GGGTCACCGG ACCCGACATG GCTTTCCACA AACCTTGGGA TTTTAACCTG TATAGAATGT 
TCGGGCATAC ATAGGGAAAT GGGGGTTCAT ATCTCTAGAA TCCAATCTCT GGAATTAGAC 
AAATTAGGGA CTTCTGAACT CTTGCTGGCC AAGAATGTAG GAAACAATAG CTTCAATGAT 
ATTATGGAAG GGAATTTGCT GTCTCCGTCA GCCAAGCCTT CTCCCTCCAG TGATATGATT 
GCACGTAAAG AATATATCAC TGCCAAGTAC GTGGAGCGGA GGTTTTCCAG AAAGATCTGT 
TCTACTGGGG CAGAAAAACT GAATGAGCTA CTTGAGGCTG TGAAATCCAG GGACTTACTT 
GCACTAATCC AAGTCTATGC AGAAGGTGTA GAGATCATGG AACCACTGCT AGAATCTGGA 
CAGGAGCCAG GTGAAACTGC ACTTCATTAT GCAGTCCGGA CAGCTGACCA CACGTCTCTT 
CCCTTGGTGG ACTTCCTGGT ACAAAACTGT GGAAATTTAG ATAAGCAGAC TGGAAAGGGC 
AACACTGCTT TACACTACTG CTGCTTATAT AACAAGCCGG AATGCTTAAA ATTGCTTTTG 
AGGGGAAAGC CAACTATTGA TGCTGTTAAC CATATTGGTG AGACGGCTTT TGATGTAGCA 
AAGAGACAGA AAGCAGCCCA GTGTGAGGAA CTGGTATCCC AAGCCCTGGC AGGGAAGCTG 
AATCCTCATG TTCACGTGGA GTACGACTGG AACCTGCGTC AGGAAGAAAT GGATGAGAGC 
GACGATGATT TGGAAGATAA GCCAAGCCCA ATAAAGAAAG AGCGTTCCCC CAGACCCCAG 
AGCTTCTGCC ATTCGTCCAG CATTTCCCCA CAAGACAAGC TGTCGCTGCC AGGTTTCAAT 
ACTCCTCGGG ACAAGCAGAG ACTCTCTTAT GGCGCCTTTA CCAACCAAAT ATTCATGTCT 
ACAAGCACAG ATTCCCCCAC GTCTCCTACT GCAGATGCCC CTCCGCTGCC CCCTAGGAAC 
GCAGGCAAAG GTAATGACTT GGGCCCGAGT GCTGCAGACA GAACTCTAAA TAAGTTTGAG 
AGATTGTCAC AGTCATCAGG CACAGGAACA ACAAAGACTG CATTAGGCCC AAGAGTCCTA 
CCAAAACTAC CTCAGAAAGT GGCACTTAGG AAAATAGACA CCACTCATCA TCTCTCAATG 
GATAAGTATA ACCAGCAACC CGAAATCTTC CAGAAATCTC CCCAGCCTGG AGATCTGCCT 
CAGAAGCCAC AAATTGCAGA TCTTCCTCCG AAACCGCAGC CTTTGGATAT CGCCCAGAAA 
CCACACGTCG GAGATCTACC TCCCAAGCCC GGAGAATTGC CTCCTAAACC TCAGCTAGGA 
GATTTGCCCC CGAAACCGCA AGATTTACCT CCAAAGCCAC AGCTGAAAGA TCTTCCGCCC 
AAGCCCAACC TGGCAGAGGT GATGTGCAAA ACTCCTGTTG CGGATATAGT CCCCAAACCT 
CATACCAATG AGGCCAATCA AAATTCCCAG CCGGTGGATG CGACCCATAA TGCACAGCCC 
ACGGACCAAA CCCCTGTCCA GAAGCAAGTT CCAGAAACTT CCAATAATGG CCCACCGCCT 
CTTCCAGAAA CACCCGTGCC ACTTCCCCGA AAGATAAACA CAGGGAAGAA TAAGCTTAAA 
CGAGTTAAAA CAATATACGA CTGCCAAGCG GACAATGATG ATGAACTCAC ATTTGCGGAG 
GGGGAAGTGA TTATCGTCAC TGGAGAGGAG GACCAGGAGT GGTGGATTGG GCACATCGAA 
GGCTTCCCGG ACAGGAAAGG AGTATTCCCT GTGTCCTTCG TCCACCTGTT GACTGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

asap1 ( XM_012964136.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819590.1 Xenopus tropicalis ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (asap1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OXa29917
Clone ID Related Accession (Same CDS sequence)	XM_012964136.2
Accession Version	XM_012964136.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012964136.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGATCTT CGGCCTCTAG ACTTTCGAGT TTTTCCTCTA GAGACTCACT ATGGAACCGG 
ATGCCCGATC AGATCTCAGT ATCGGAGTTT ATATCGGAGA CCACAGAGGA TTACAATTCG 
CCAACCACGT CCAGTTTCAC GACCCGTCTT CAGAGCTGCA GGAATTCGAT CAATGTGCTG 
GAAGAGGCCT TAGATCAAGA TAGGACAGCG TTGCAGAAAG TGAAAAAATC GGTGAAAGCA 
ATTTATAATT CAGGCCAAGA TCATGTCCAA AATGAAGAAA ACTATGCACA AGCCTTAGAT 
AAATTTGGAA GTAATTTTCT AAGTCGGGAT AACGCAGATT TGGGCACCGC CTTCGTGAAA 
TTCTCCACTC TAACAAAAGA ACTATCAACG CTTCTTAAAA ACCTGCTACA GGGATTAAGC 
CACAATGTCA TCTTCACCTT GGATTCTCTC TTGAAAGGGG ACTTAAAGGG CGTAAAAGGG 
GACCTTAAGA AGCCATTCGA CAGAGCGTGG AAGGATTATG AAACCAAATT CACGAAAATC 
GAAAAGGAGA AAAGGGAACA CGCCAAGCAA CATGGGATGA TCCGTACGGA GATTACTGGG 
GCCGAGATTG CCGAAGAGAT GGAGAAGGAG AGGAGATTAT TCCAGCTACA GATGTGTGAG 
TATCTCATTA AAGTTAATGA AATCAAGACT AAGAAAGGCG TGGATCTGCT GCAGAACCTT 
ATAAAGTACT ATCACGCACA GTGCAATTTC TTCCAGGATG GATTGAAAAC AGCTGACAAG 
CTGAAGCAGT ACATAGAGAA ATTGGCTGCA GATTTGTACA ATATTAAGCA GACCCAAGAT 
GAGGAGAAGA AACAGCTCAC TGCCCTGCGA GACCTGATAA AATCCTCTTT ACAGCTGGAT 
CAGAAGGAGG ATTCCCAGAG TAAGCAGGCA GGCTACAGCA TGCACCAGCT CCAGGGGAAC 
AAGGAGTATG GCAGCGAGAA GAAAGGCTAC CTGCTGAAAA AAAGTGATGG CATCCGGAAA 
GTATGGCAGA GGAGGAAGTG CACTGTGAAA AATGGAATCC TGACCATTTC CCATGCAACG 
TCAAACAGGC CACCGACGAA GCTGAACCTG CTGACCTGCC AAGTCAAGCC GAACGCTGAT 
GAGAAGAAAT GCTTTGATCT CATCTCACAC AACAGAACAT ACCACTTCCA AGCAGAGGAC 
GAACAAGAAT ACATTGCGTG GATATCAGTT CTGACAAATA GCAAAGAAGA TGCACTCAAT 
ATGGCTTTCC GGGGAGACCA GAGCACTGGC GAGAGCAGCC TGGAGGATCT GACTAAGGCC 
ATTATAGACG ATGTACAGAA AACGCCTGGA AATGAAGTTT GCTGTGATTG TGGGTCACCG 
GACCCGACAT GGCTTTCCAC AAACCTTGGG ATTTTAACCT GTATAGAATG TTCGGGCATA 
CATAGGGAAA TGGGGGTTCA TATCTCTAGA ATCCAATCTC TGGAATTAGA CAAATTAGGG 
ACTTCTGAAC TCTTGCTGGC CAAGAATGTA GGAAACAATA GCTTCAATGA TATTATGGAA 
GGGAATTTGC TGTCTCCGTC AGCCAAGCCT TCTCCCTCCA GTGATATGAT TGCACGTAAA 
GAATATATCA CTGCCAAGTA CGTGGAGCGG AGGTTTTCCA GAAAGATCTG TTCTACTGGG 
GCAGAAAAAC TGAATGAGCT ACTTGAGGCT GTGAAATCCA GGGACTTACT TGCACTAATC 
CAAGTCTATG CAGAAGGTGT AGAGATCATG GAACCACTGC TAGAATCTGG ACAGGAGCCA 
GGTGAAACTG CACTTCATTA TGCAGTCCGG ACAGCTGACC ACACGTCTCT TCCCTTGGTG 
GACTTCCTGG TACAAAACTG TGGAAATTTA GATAAGCAGA CTGGAAAGGG CAACACTGCT 
TTACACTACT GCTGCTTATA TAACAAGCCG GAATGCTTAA AATTGCTTTT GAGGGGAAAG 
CCAACTATTG ATGCTGTTAA CCATATTGGT GAGACGGCTT TTGATGTAGC AAAGAGACAG 
AAAGCAGCCC AGTGTGAGGA ACTGGTATCC CAAGCCCTGG CAGGGAAGCT GAATCCTCAT 
GTTCACGTGG AGTACGACTG GAACCTGCGT CAGGAAGAAA TGGATGAGAG CGACGATGAT 
TTGGAAGATA AGCCAAGCCC AATAAAGAAA GAGCGTTCCC CCAGACCCCA GAGCTTCTGC 
CATTCGTCCA GCATTTCCCC ACAAGACAAG CTGTCGCTGC CAGGTTTCAA TACTCCTCGG 
GACAAGCAGA GACTCTCTTA TGGCGCCTTT ACCAACCAAA TATTCATGTC TACAAGCACA 
GATTCCCCCA CGTCTCCTAC TGCAGATGCC CCTCCGCTGC CCCCTAGGAA CGCAGGCAAA 
GGTAATGACT TGGGCCCGAG TGCTGCAGAC AGAACTCTAA ATAAGTTTGA GAGATTGTCA 
CAGTCATCAG GCACAGGAAC AACAAAGACT GCATTAGGCC CAAGAGTCCT ACCAAAACTA 
CCTCAGAAAG TGGCACTTAG GAAAATAGAC ACCACTCATC ATCTCTCAAT GGATAAGTAT 
AACCAGCAAC CCGAAATCTT CCAGAAATCT CCCCAGCCTG GAGATCTGCC TCAGAAGCCA 
CAAATTGCAG ATCTTCCTCC GAAACCGCAG CCTTTGGATA TCGCCCAGAA ACCACACGTC 
GGAGATCTAC CTCCCAAGCC CGGAGAATTG CCTCCTAAAC CTCAGCTAGG AGATTTGCCC 
CCGAAACCGC AAGATTTACC TCCAAAGCCA CAGCTGAAAG ATCTTCCGCC CAAGCCCAAC 
CTGGCAGAGG TGATGTGCAA AACTCCTGTT GCGGATATAG TCCCCAAACC TCATACCAAT 
GAGGCCAATC AAAATTCCCA GCCGGTGGAT GCGACCCATA ATGCACAGCC CACGGACCAA 
ACCCCTGTCC AGAAGCAAGT TCCAGAAACT TCCAATAATG GCCCACCGCC TCTTCCAGAA 
ACACCCGTGC CACTTCCCCG AAAGATAAAC ACAGGGAAGA ATAAGCTTAA ACGAGTTAAA 
ACAATATACG ACTGCCAAGC GGACAATGAT GATGAACTCA CATTTGCGGA GGGGGAAGTG 
ATTATCGTCA CTGGAGAGGA GGACCAGGAG TGGTGGATTG GGCACATCGA AGGCTTCCCG 
GACAGGAAAG GAGTATTCCC TGTGTCCTTC GTCCACCTGT TGACTGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819590.1
CDS	30..3260
Translation	MRSSASRLSSFSSRDSLWNRMPDQISVSEFISETTEDYNSPTTSSFTTRLQSCRNSINVLEEALDQDRTALQKVKKSVKAIYNSGQDHVQNEENYAQALDKFGSNFLSRDNADLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGVKGDLKKPFDRAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDQKEDSQSKQAGYSMHQLQGNKEYGSEKKGYLLKKSDGIRKVWQRRKCTVKNGILTISHATSNRPPTKLNLLTCQVKPNADEKKCFDLISHNRTYHFQAEDEQEYIAWISVLTNSKEDALNMAFRGDQSTGESSLEDLTKAIIDDVQKTPGNEVCCDCGSPDPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDKLGTSELLLAKNVGNNSFNDIMEGNLLSPSAKPSPSSDMIARKEYITAKYVERRFSRKICSTGAEKLNELLEAVKSRDLLALIQVYAEGVEIMEPLLESGQEPGETALHYAVRTADHTSLPLVDFLVQNCGNLDKQTGKGNTALHYCCLYNKPECLKLLLRGKPTIDAVNHIGETAFDVAKRQKAAQCEELVSQALAGKLNPHVHVEYDWNLRQEEMDESDDDLEDKPSPIKKERSPRPQSFCHSSSISPQDKLSLPGFNTPRDKQRLSYGAFTNQIFMSTSTDSPTSPTADAPPLPPRNAGKGNDLGPSAADRTLNKFERLSQSSGTGTTKTALGPRVLPKLPQKVALRKIDTTHHLSMDKYNQQPEIFQKSPQPGDLPQKPQIADLPPKPQPLDIAQKPHVGDLPPKPGELPPKPQLGDLPPKPQDLPPKPQLKDLPPKPNLAEVMCKTPVADIVPKPHTNEANQNSQPVDATHNAQPTDQTPVQKQVPETSNNGPPPLPETPVPLPRKINTGKNKLKRVKTIYDCQADNDDELTFAEGEVIIVTGEEDQEWWIGHIEGFPDRKGVFPVSFVHLLTE

Target ORF information:

RefSeq Version	XM_012964136.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (asap1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012964136.2

ORF Insert Sequence:

ATGCGATCTT CGGCCTCTAG ACTTTCGAGT TTTTCCTCTA GAGACTCACT ATGGAACCGG 
ATGCCCGATC AGATCTCAGT ATCGGAGTTT ATATCGGAGA CCACAGAGGA TTACAATTCG 
CCAACCACGT CCAGTTTCAC GACCCGTCTT CAGAGCTGCA GGAATTCGAT CAATGTGCTG 
GAAGAGGCCT TAGATCAAGA TAGGACAGCG TTGCAGAAAG TGAAAAAATC GGTGAAAGCA 
ATTTATAATT CAGGCCAAGA TCATGTCCAA AATGAAGAAA ACTATGCACA AGCCTTAGAT 
AAATTTGGAA GTAATTTTCT AAGTCGGGAT AACGCAGATT TGGGCACCGC CTTCGTGAAA 
TTCTCCACTC TAACAAAAGA ACTATCAACG CTTCTTAAAA ACCTGCTACA GGGATTAAGC 
CACAATGTCA TCTTCACCTT GGATTCTCTC TTGAAAGGGG ACTTAAAGGG CGTAAAAGGG 
GACCTTAAGA AGCCATTCGA CAGAGCGTGG AAGGATTATG AAACCAAATT CACGAAAATC 
GAAAAGGAGA AAAGGGAACA CGCCAAGCAA CATGGGATGA TCCGTACGGA GATTACTGGG 
GCCGAGATTG CCGAAGAGAT GGAGAAGGAG AGGAGATTAT TCCAGCTACA GATGTGTGAG 
TATCTCATTA AAGTTAATGA AATCAAGACT AAGAAAGGCG TGGATCTGCT GCAGAACCTT 
ATAAAGTACT ATCACGCACA GTGCAATTTC TTCCAGGATG GATTGAAAAC AGCTGACAAG 
CTGAAGCAGT ACATAGAGAA ATTGGCTGCA GATTTGTACA ATATTAAGCA GACCCAAGAT 
GAGGAGAAGA AACAGCTCAC TGCCCTGCGA GACCTGATAA AATCCTCTTT ACAGCTGGAT 
CAGAAGGAGG ATTCCCAGAG TAAGCAGGCA GGCTACAGCA TGCACCAGCT CCAGGGGAAC 
AAGGAGTATG GCAGCGAGAA GAAAGGCTAC CTGCTGAAAA AAAGTGATGG CATCCGGAAA 
GTATGGCAGA GGAGGAAGTG CACTGTGAAA AATGGAATCC TGACCATTTC CCATGCAACG 
TCAAACAGGC CACCGACGAA GCTGAACCTG CTGACCTGCC AAGTCAAGCC GAACGCTGAT 
GAGAAGAAAT GCTTTGATCT CATCTCACAC AACAGAACAT ACCACTTCCA AGCAGAGGAC 
GAACAAGAAT ACATTGCGTG GATATCAGTT CTGACAAATA GCAAAGAAGA TGCACTCAAT 
ATGGCTTTCC GGGGAGACCA GAGCACTGGC GAGAGCAGCC TGGAGGATCT GACTAAGGCC 
ATTATAGACG ATGTACAGAA AACGCCTGGA AATGAAGTTT GCTGTGATTG TGGGTCACCG 
GACCCGACAT GGCTTTCCAC AAACCTTGGG ATTTTAACCT GTATAGAATG TTCGGGCATA 
CATAGGGAAA TGGGGGTTCA TATCTCTAGA ATCCAATCTC TGGAATTAGA CAAATTAGGG 
ACTTCTGAAC TCTTGCTGGC CAAGAATGTA GGAAACAATA GCTTCAATGA TATTATGGAA 
GGGAATTTGC TGTCTCCGTC AGCCAAGCCT TCTCCCTCCA GTGATATGAT TGCACGTAAA 
GAATATATCA CTGCCAAGTA CGTGGAGCGG AGGTTTTCCA GAAAGATCTG TTCTACTGGG 
GCAGAAAAAC TGAATGAGCT ACTTGAGGCT GTGAAATCCA GGGACTTACT TGCACTAATC 
CAAGTCTATG CAGAAGGTGT AGAGATCATG GAACCACTGC TAGAATCTGG ACAGGAGCCA 
GGTGAAACTG CACTTCATTA TGCAGTCCGG ACAGCTGACC ACACGTCTCT TCCCTTGGTG 
GACTTCCTGG TACAAAACTG TGGAAATTTA GATAAGCAGA CTGGAAAGGG CAACACTGCT 
TTACACTACT GCTGCTTATA TAACAAGCCG GAATGCTTAA AATTGCTTTT GAGGGGAAAG 
CCAACTATTG ATGCTGTTAA CCATATTGGT GAGACGGCTT TTGATGTAGC AAAGAGACAG 
AAAGCAGCCC AGTGTGAGGA ACTGGTATCC CAAGCCCTGG CAGGGAAGCT GAATCCTCAT 
GTTCACGTGG AGTACGACTG GAACCTGCGT CAGGAAGAAA TGGATGAGAG CGACGATGAT 
TTGGAAGATA AGCCAAGCCC AATAAAGAAA GAGCGTTCCC CCAGACCCCA GAGCTTCTGC 
CATTCGTCCA GCATTTCCCC ACAAGACAAG CTGTCGCTGC CAGGTTTCAA TACTCCTCGG 
GACAAGCAGA GACTCTCTTA TGGCGCCTTT ACCAACCAAA TATTCATGTC TACAAGCACA 
GATTCCCCCA CGTCTCCTAC TGCAGATGCC CCTCCGCTGC CCCCTAGGAA CGCAGGCAAA 
GGTAATGACT TGGGCCCGAG TGCTGCAGAC AGAACTCTAA ATAAGTTTGA GAGATTGTCA 
CAGTCATCAG GCACAGGAAC AACAAAGACT GCATTAGGCC CAAGAGTCCT ACCAAAACTA 
CCTCAGAAAG TGGCACTTAG GAAAATAGAC ACCACTCATC ATCTCTCAAT GGATAAGTAT 
AACCAGCAAC CCGAAATCTT CCAGAAATCT CCCCAGCCTG GAGATCTGCC TCAGAAGCCA 
CAAATTGCAG ATCTTCCTCC GAAACCGCAG CCTTTGGATA TCGCCCAGAA ACCACACGTC 
GGAGATCTAC CTCCCAAGCC CGGAGAATTG CCTCCTAAAC CTCAGCTAGG AGATTTGCCC 
CCGAAACCGC AAGATTTACC TCCAAAGCCA CAGCTGAAAG ATCTTCCGCC CAAGCCCAAC 
CTGGCAGAGG TGATGTGCAA AACTCCTGTT GCGGATATAG TCCCCAAACC TCATACCAAT 
GAGGCCAATC AAAATTCCCA GCCGGTGGAT GCGACCCATA ATGCACAGCC CACGGACCAA 
ACCCCTGTCC AGAAGCAAGT TCCAGAAACT TCCAATAATG GCCCACCGCC TCTTCCAGAA 
ACACCCGTGC CACTTCCCCG AAAGATAAAC ACAGGGAAGA ATAAGCTTAA ACGAGTTAAA 
ACAATATACG ACTGCCAAGC GGACAATGAT GATGAACTCA CATTTGCGGA GGGGGAAGTG 
ATTATCGTCA CTGGAGAGGA GGACCAGGAG TGGTGGATTG GGCACATCGA AGGCTTCCCG 
GACAGGAAAG GAGTATTCCC TGTGTCCTTC GTCCACCTGT TGACTGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

asap1 ( XM_012964137.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819591.1 Xenopus tropicalis ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (asap1), transcript variant X7, mRNA.

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CloneID	OXa29918
Clone ID Related Accession (Same CDS sequence)	XM_012964137.2
Accession Version	XM_012964137.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3183bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012964137.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCCCG ATCAGATCTC AGTATCGGAG TTTATATCGG AGACCACAGA GGATTACAAT 
TCGCCAACCA CGTCCAGTTT CACGACCCGT CTTCAGAGCT GCAGGAATTC GATCAATGTG 
CTGGAAGAGG CCTTAGATCA AGATAGGACA GCGTTGCAGA AAGTGAAAAA ATCGGTGAAA 
GCAATTTATA ATTCAGGCCA AGATCATGTC CAAAATGAAG AAAACTATGC ACAAGCCTTA 
GATAAATTTG GAAGTAATTT TCTAAGTCGG GATAACGCAG ATTTGGGCAC CGCCTTCGTG 
AAATTCTCCA CTCTAACAAA AGAACTATCA ACGCTTCTTA AAAACCTGCT ACAGGGATTA 
AGCCACAATG TCATCTTCAC CTTGGATTCT CTCTTGAAAG GGGACTTAAA GGGCGTAAAA 
GGGGACCTTA AGAAGCCATT CGACAGAGCG TGGAAGGATT ATGAAACCAA ATTCACGAAA 
ATCGAAAAGG AGAAAAGGGA ACACGCCAAG CAACATGGGA TGATCCGTAC GGAGATTACT 
GGGGCCGAGA TTGCCGAAGA GATGGAGAAG GAGAGGAGAT TATTCCAGCT ACAGATGTGT 
GAGTATCTCA TTAAAGTTAA TGAAATCAAG ACTAAGAAAG GCGTGGATCT GCTGCAGAAC 
CTTATAAAGT ACTATCACGC ACAGTGCAAT TTCTTCCAGG ATGGATTGAA AACAGCTGAC 
AAGCTGAAGC AGTACATAGA GAAATTGGCT GCAGATTTGT ACAATATTAA GCAGACCCAA 
GATGAGGAGA AGAAACAGCT CACTGCCCTG CGAGACCTGA TAAAATCCTC TTTACAGCTG 
GATCAGAAGG AGTCTAGAAG AGATTCCCAG AGTAAGCAGG CAGGCTACAG CATGCACCAG 
CTCCAGGGGA ACAAGGAGTA TGGCAGCGAG AAGAAAGGCT ACCTGCTGAA AAAAAGTGAT 
GGCATCCGGA AAGTATGGCA GAGGAGGAAG TGCACTGTGA AAAATGGAAT CCTGACCATT 
TCCCATGCAA CGTCAAACAG GCCACCGACG AAGCTGAACC TGCTGACCTG CCAAGTCAAG 
CCGAACGCTG ATGAGAAGAA ATGCTTTGAT CTCATCTCAC ACAACAGAAC ATACCACTTC 
CAAGCAGAGG ACGAACAAGA ATACATTGCG TGGATATCAG TTCTGACAAA TAGCAAAGAA 
GATGCACTCA ATATGGCTTT CCGGGGAGAC CAGAGCACTG GCGAGAGCAG CCTGGAGGAT 
CTGACTAAGG CCATTATAGA CGATGTACAG AAAACGCCTG GAAATGAAGT TTGCTGTGAT 
TGTGGGTCAC CGGACCCGAC ATGGCTTTCC ACAAACCTTG GGATTTTAAC CTGTATAGAA 
TGTTCGGGCA TACATAGGGA AATGGGGGTT CATATCTCTA GAATCCAATC TCTGGAATTA 
GACAAATTAG GGACTTCTGA ACTCTTGCTG GCCAAGAATG TAGGAAACAA TAGCTTCAAT 
GATATTATGG AAGGGAATTT GCTGTCTCCG TCAGCCAAGC CTTCTCCCTC CAGTGATATG 
ATTGCACGTA AAGAATATAT CACTGCCAAG TACGTGGAGC GGAGGTTTTC CAGAAAGATC 
TGTTCTACTG GGGCAGAAAA ACTGAATGAG CTACTTGAGG CTGTGAAATC CAGGGACTTA 
CTTGCACTAA TCCAAGTCTA TGCAGAAGGT GTAGAGATCA TGGAACCACT GCTAGAATCT 
GGACAGGAGC CAGGTGAAAC TGCACTTCAT TATGCAGTCC GGACAGCTGA CCACACGTCT 
CTTCCCTTGG TGGACTTCCT GGTACAAAAC TGTGGAAATT TAGATAAGCA GACTGGAAAG 
GGCAACACTG CTTTACACTA CTGCTGCTTA TATAACAAGC CGGAATGCTT AAAATTGCTT 
TTGAGGGGAA AGCCAACTAT TGATGCTGTT AACCATATTG GTGAGACGGC TTTTGATGTA 
GCAAAGAGAC AGAAAGCAGC CCAGTGTGAG GAACTGGTAT CCCAAGCCCT GGCAGGGAAG 
CTGAATCCTC ATGTTCACGT GGAGTACGAC TGGAACCTGC GTCAGGAAGA AATGGATGAG 
AGCGACGATG ATTTGGAAGA TAAGCCAAGC CCAATAAAGA AAGAGCGTTC CCCCAGACCC 
CAGAGCTTCT GCCATTCGTC CAGCATTTCC CCACAAGACA AGCTGTCGCT GCCAGGTTTC 
AATACTCCTC GGGACAAGCA GAGACTCTCT TATGGCGCCT TTACCAACCA AATATTCATG 
TCTACAAGCA CAGATTCCCC CACGTCTCCT ACTGCAGATG CCCCTCCGCT GCCCCCTAGG 
AACGCAGGCA AAGGTAATGA CTTGGGCCCG AGTGCTGCAG ACAGAACTCT AAATAAGTTT 
GAGAGATTGT CACAGTCATC AGGCACAGGA ACAACAAAGA CTGCATTAGG CCCAAGAGTC 
CTACCAAAAC TACCTCAGAA AGTGGCACTT AGGAAAATAG ACACCACTCA TCATCTCTCA 
ATGGATAAGT ATAACCAGCA ACCCGAAATC TTCCAGAAAT CTCCCCAGCC TGGAGATCTG 
CCTCAGAAGC CACAAATTGC AGATCTTCCT CCGAAACCGC AGCCTTTGGA TATCGCCCAG 
AAACCACACG TCGGAGATCT ACCTCCCAAG CCCGGAGAAT TGCCTCCTAA ACCTCAGCTA 
GGAGATTTGC CCCCGAAACC GCAAGATTTA CCTCCAAAGC CACAGCTGAA AGATCTTCCG 
CCCAAGCCCA ACCTGGCAGA GGTGATGTGC AAAACTCCTG TTGCGGATAT AGTCCCCAAA 
CCTCATACCA ATGAGGCCAA TCAAAATTCC CAGCCGGTGG ATGCGACCCA TAATGCACAG 
CCCACGGACC AAACCCCTGT CCAGAAGCAA GTTCCAGAAA CTTCCAATAA TGGCCCACCG 
CCTCTTCCAG AAACACCCGT GCCACTTCCC CGAAAGATAA ACACAGGGAA GAATAAGCTT 
AAACGAGTTA AAACAATATA CGACTGCCAA GCGGACAATG ATGATGAACT CACATTTGCG 
GAGGGGGAAG TGATTATCGT CACTGGAGAG GAGGACCAGG AGTGGTGGAT TGGGCACATC 
GAAGGCTTCC CGGACAGGAA AGGAGTATTC CCTGTGTCCT TCGTCCACCT GTTGACTGAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819591.1
CDS	498..3680
Translation	MMPDQISVSEFISETTEDYNSPTTSSFTTRLQSCRNSINVLEEALDQDRTALQKVKKSVKAIYNSGQDHVQNEENYAQALDKFGSNFLSRDNADLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGVKGDLKKPFDRAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDQKESRRDSQSKQAGYSMHQLQGNKEYGSEKKGYLLKKSDGIRKVWQRRKCTVKNGILTISHATSNRPPTKLNLLTCQVKPNADEKKCFDLISHNRTYHFQAEDEQEYIAWISVLTNSKEDALNMAFRGDQSTGESSLEDLTKAIIDDVQKTPGNEVCCDCGSPDPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDKLGTSELLLAKNVGNNSFNDIMEGNLLSPSAKPSPSSDMIARKEYITAKYVERRFSRKICSTGAEKLNELLEAVKSRDLLALIQVYAEGVEIMEPLLESGQEPGETALHYAVRTADHTSLPLVDFLVQNCGNLDKQTGKGNTALHYCCLYNKPECLKLLLRGKPTIDAVNHIGETAFDVAKRQKAAQCEELVSQALAGKLNPHVHVEYDWNLRQEEMDESDDDLEDKPSPIKKERSPRPQSFCHSSSISPQDKLSLPGFNTPRDKQRLSYGAFTNQIFMSTSTDSPTSPTADAPPLPPRNAGKGNDLGPSAADRTLNKFERLSQSSGTGTTKTALGPRVLPKLPQKVALRKIDTTHHLSMDKYNQQPEIFQKSPQPGDLPQKPQIADLPPKPQPLDIAQKPHVGDLPPKPGELPPKPQLGDLPPKPQDLPPKPQLKDLPPKPNLAEVMCKTPVADIVPKPHTNEANQNSQPVDATHNAQPTDQTPVQKQVPETSNNGPPPLPETPVPLPRKINTGKNKLKRVKTIYDCQADNDDELTFAEGEVIIVTGEEDQEWWIGHIEGFPDRKGVFPVSFVHLLTE

Target ORF information:

RefSeq Version	XM_012964137.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (asap1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012964137.2

ORF Insert Sequence:

ATGATGCCCG ATCAGATCTC AGTATCGGAG TTTATATCGG AGACCACAGA GGATTACAAT 
TCGCCAACCA CGTCCAGTTT CACGACCCGT CTTCAGAGCT GCAGGAATTC GATCAATGTG 
CTGGAAGAGG CCTTAGATCA AGATAGGACA GCGTTGCAGA AAGTGAAAAA ATCGGTGAAA 
GCAATTTATA ATTCAGGCCA AGATCATGTC CAAAATGAAG AAAACTATGC ACAAGCCTTA 
GATAAATTTG GAAGTAATTT TCTAAGTCGG GATAACGCAG ATTTGGGCAC CGCCTTCGTG 
AAATTCTCCA CTCTAACAAA AGAACTATCA ACGCTTCTTA AAAACCTGCT ACAGGGATTA 
AGCCACAATG TCATCTTCAC CTTGGATTCT CTCTTGAAAG GGGACTTAAA GGGCGTAAAA 
GGGGACCTTA AGAAGCCATT CGACAGAGCG TGGAAGGATT ATGAAACCAA ATTCACGAAA 
ATCGAAAAGG AGAAAAGGGA ACACGCCAAG CAACATGGGA TGATCCGTAC GGAGATTACT 
GGGGCCGAGA TTGCCGAAGA GATGGAGAAG GAGAGGAGAT TATTCCAGCT ACAGATGTGT 
GAGTATCTCA TTAAAGTTAA TGAAATCAAG ACTAAGAAAG GCGTGGATCT GCTGCAGAAC 
CTTATAAAGT ACTATCACGC ACAGTGCAAT TTCTTCCAGG ATGGATTGAA AACAGCTGAC 
AAGCTGAAGC AGTACATAGA GAAATTGGCT GCAGATTTGT ACAATATTAA GCAGACCCAA 
GATGAGGAGA AGAAACAGCT CACTGCCCTG CGAGACCTGA TAAAATCCTC TTTACAGCTG 
GATCAGAAGG AGTCTAGAAG AGATTCCCAG AGTAAGCAGG CAGGCTACAG CATGCACCAG 
CTCCAGGGGA ACAAGGAGTA TGGCAGCGAG AAGAAAGGCT ACCTGCTGAA AAAAAGTGAT 
GGCATCCGGA AAGTATGGCA GAGGAGGAAG TGCACTGTGA AAAATGGAAT CCTGACCATT 
TCCCATGCAA CGTCAAACAG GCCACCGACG AAGCTGAACC TGCTGACCTG CCAAGTCAAG 
CCGAACGCTG ATGAGAAGAA ATGCTTTGAT CTCATCTCAC ACAACAGAAC ATACCACTTC 
CAAGCAGAGG ACGAACAAGA ATACATTGCG TGGATATCAG TTCTGACAAA TAGCAAAGAA 
GATGCACTCA ATATGGCTTT CCGGGGAGAC CAGAGCACTG GCGAGAGCAG CCTGGAGGAT 
CTGACTAAGG CCATTATAGA CGATGTACAG AAAACGCCTG GAAATGAAGT TTGCTGTGAT 
TGTGGGTCAC CGGACCCGAC ATGGCTTTCC ACAAACCTTG GGATTTTAAC CTGTATAGAA 
TGTTCGGGCA TACATAGGGA AATGGGGGTT CATATCTCTA GAATCCAATC TCTGGAATTA 
GACAAATTAG GGACTTCTGA ACTCTTGCTG GCCAAGAATG TAGGAAACAA TAGCTTCAAT 
GATATTATGG AAGGGAATTT GCTGTCTCCG TCAGCCAAGC CTTCTCCCTC CAGTGATATG 
ATTGCACGTA AAGAATATAT CACTGCCAAG TACGTGGAGC GGAGGTTTTC CAGAAAGATC 
TGTTCTACTG GGGCAGAAAA ACTGAATGAG CTACTTGAGG CTGTGAAATC CAGGGACTTA 
CTTGCACTAA TCCAAGTCTA TGCAGAAGGT GTAGAGATCA TGGAACCACT GCTAGAATCT 
GGACAGGAGC CAGGTGAAAC TGCACTTCAT TATGCAGTCC GGACAGCTGA CCACACGTCT 
CTTCCCTTGG TGGACTTCCT GGTACAAAAC TGTGGAAATT TAGATAAGCA GACTGGAAAG 
GGCAACACTG CTTTACACTA CTGCTGCTTA TATAACAAGC CGGAATGCTT AAAATTGCTT 
TTGAGGGGAA AGCCAACTAT TGATGCTGTT AACCATATTG GTGAGACGGC TTTTGATGTA 
GCAAAGAGAC AGAAAGCAGC CCAGTGTGAG GAACTGGTAT CCCAAGCCCT GGCAGGGAAG 
CTGAATCCTC ATGTTCACGT GGAGTACGAC TGGAACCTGC GTCAGGAAGA AATGGATGAG 
AGCGACGATG ATTTGGAAGA TAAGCCAAGC CCAATAAAGA AAGAGCGTTC CCCCAGACCC 
CAGAGCTTCT GCCATTCGTC CAGCATTTCC CCACAAGACA AGCTGTCGCT GCCAGGTTTC 
AATACTCCTC GGGACAAGCA GAGACTCTCT TATGGCGCCT TTACCAACCA AATATTCATG 
TCTACAAGCA CAGATTCCCC CACGTCTCCT ACTGCAGATG CCCCTCCGCT GCCCCCTAGG 
AACGCAGGCA AAGGTAATGA CTTGGGCCCG AGTGCTGCAG ACAGAACTCT AAATAAGTTT 
GAGAGATTGT CACAGTCATC AGGCACAGGA ACAACAAAGA CTGCATTAGG CCCAAGAGTC 
CTACCAAAAC TACCTCAGAA AGTGGCACTT AGGAAAATAG ACACCACTCA TCATCTCTCA 
ATGGATAAGT ATAACCAGCA ACCCGAAATC TTCCAGAAAT CTCCCCAGCC TGGAGATCTG 
CCTCAGAAGC CACAAATTGC AGATCTTCCT CCGAAACCGC AGCCTTTGGA TATCGCCCAG 
AAACCACACG TCGGAGATCT ACCTCCCAAG CCCGGAGAAT TGCCTCCTAA ACCTCAGCTA 
GGAGATTTGC CCCCGAAACC GCAAGATTTA CCTCCAAAGC CACAGCTGAA AGATCTTCCG 
CCCAAGCCCA ACCTGGCAGA GGTGATGTGC AAAACTCCTG TTGCGGATAT AGTCCCCAAA 
CCTCATACCA ATGAGGCCAA TCAAAATTCC CAGCCGGTGG ATGCGACCCA TAATGCACAG 
CCCACGGACC AAACCCCTGT CCAGAAGCAA GTTCCAGAAA CTTCCAATAA TGGCCCACCG 
CCTCTTCCAG AAACACCCGT GCCACTTCCC CGAAAGATAA ACACAGGGAA GAATAAGCTT 
AAACGAGTTA AAACAATATA CGACTGCCAA GCGGACAATG ATGATGAACT CACATTTGCG 
GAGGGGGAAG TGATTATCGT CACTGGAGAG GAGGACCAGG AGTGGTGGAT TGGGCACATC 
GAAGGCTTCC CGGACAGGAA AGGAGTATTC CCTGTGTCCT TCGTCCACCT GTTGACTGAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

asap1 ( XM_012964138.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819592.1 Xenopus tropicalis ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (asap1), transcript variant X8, mRNA.

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CloneID	OXa29919
Clone ID Related Accession (Same CDS sequence)	XM_012964138.2
Accession Version	XM_012964138.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3174bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012964138.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCCCG ATCAGATCTC AGTATCGGAG TTTATATCGG AGACCACAGA GGATTACAAT 
TCGCCAACCA CGTCCAGTTT CACGACCCGT CTTCAGAGCT GCAGGAATTC GATCAATGTG 
CTGGAAGAGG CCTTAGATCA AGATAGGACA GCGTTGCAGA AAGTGAAAAA ATCGGTGAAA 
GCAATTTATA ATTCAGGCCA AGATCATGTC CAAAATGAAG AAAACTATGC ACAAGCCTTA 
GATAAATTTG GAAGTAATTT TCTAAGTCGG GATAACGCAG ATTTGGGCAC CGCCTTCGTG 
AAATTCTCCA CTCTAACAAA AGAACTATCA ACGCTTCTTA AAAACCTGCT ACAGGGATTA 
AGCCACAATG TCATCTTCAC CTTGGATTCT CTCTTGAAAG GGGACTTAAA GGGCGTAAAA 
GGGGACCTTA AGAAGCCATT CGACAGAGCG TGGAAGGATT ATGAAACCAA ATTCACGAAA 
ATCGAAAAGG AGAAAAGGGA ACACGCCAAG CAACATGGGA TGATCCGTAC GGAGATTACT 
GGGGCCGAGA TTGCCGAAGA GATGGAGAAG GAGAGGAGAT TATTCCAGCT ACAGATGTGT 
GAGTATCTCA TTAAAGTTAA TGAAATCAAG ACTAAGAAAG GCGTGGATCT GCTGCAGAAC 
CTTATAAAGT ACTATCACGC ACAGTGCAAT TTCTTCCAGG ATGGATTGAA AACAGCTGAC 
AAGCTGAAGC AGTACATAGA GAAATTGGCT GCAGATTTGT ACAATATTAA GCAGACCCAA 
GATGAGGAGA AGAAACAGCT CACTGCCCTG CGAGACCTGA TAAAATCCTC TTTACAGCTG 
GATCAGAAGG AGGATTCCCA GAGTAAGCAG GCAGGCTACA GCATGCACCA GCTCCAGGGG 
AACAAGGAGT ATGGCAGCGA GAAGAAAGGC TACCTGCTGA AAAAAAGTGA TGGCATCCGG 
AAAGTATGGC AGAGGAGGAA GTGCACTGTG AAAAATGGAA TCCTGACCAT TTCCCATGCA 
ACGTCAAACA GGCCACCGAC GAAGCTGAAC CTGCTGACCT GCCAAGTCAA GCCGAACGCT 
GATGAGAAGA AATGCTTTGA TCTCATCTCA CACAACAGAA CATACCACTT CCAAGCAGAG 
GACGAACAAG AATACATTGC GTGGATATCA GTTCTGACAA ATAGCAAAGA AGATGCACTC 
AATATGGCTT TCCGGGGAGA CCAGAGCACT GGCGAGAGCA GCCTGGAGGA TCTGACTAAG 
GCCATTATAG ACGATGTACA GAAAACGCCT GGAAATGAAG TTTGCTGTGA TTGTGGGTCA 
CCGGACCCGA CATGGCTTTC CACAAACCTT GGGATTTTAA CCTGTATAGA ATGTTCGGGC 
ATACATAGGG AAATGGGGGT TCATATCTCT AGAATCCAAT CTCTGGAATT AGACAAATTA 
GGGACTTCTG AACTCTTGCT GGCCAAGAAT GTAGGAAACA ATAGCTTCAA TGATATTATG 
GAAGGGAATT TGCTGTCTCC GTCAGCCAAG CCTTCTCCCT CCAGTGATAT GATTGCACGT 
AAAGAATATA TCACTGCCAA GTACGTGGAG CGGAGGTTTT CCAGAAAGAT CTGTTCTACT 
GGGGCAGAAA AACTGAATGA GCTACTTGAG GCTGTGAAAT CCAGGGACTT ACTTGCACTA 
ATCCAAGTCT ATGCAGAAGG TGTAGAGATC ATGGAACCAC TGCTAGAATC TGGACAGGAG 
CCAGGTGAAA CTGCACTTCA TTATGCAGTC CGGACAGCTG ACCACACGTC TCTTCCCTTG 
GTGGACTTCC TGGTACAAAA CTGTGGAAAT TTAGATAAGC AGACTGGAAA GGGCAACACT 
GCTTTACACT ACTGCTGCTT ATATAACAAG CCGGAATGCT TAAAATTGCT TTTGAGGGGA 
AAGCCAACTA TTGATGCTGT TAACCATATT GGTGAGACGG CTTTTGATGT AGCAAAGAGA 
CAGAAAGCAG CCCAGTGTGA GGAACTGGTA TCCCAAGCCC TGGCAGGGAA GCTGAATCCT 
CATGTTCACG TGGAGTACGA CTGGAACCTG CGTCAGGAAG AAATGGATGA GAGCGACGAT 
GATTTGGAAG ATAAGCCAAG CCCAATAAAG AAAGAGCGTT CCCCCAGACC CCAGAGCTTC 
TGCCATTCGT CCAGCATTTC CCCACAAGAC AAGCTGTCGC TGCCAGGTTT CAATACTCCT 
CGGGACAAGC AGAGACTCTC TTATGGCGCC TTTACCAACC AAATATTCAT GTCTACAAGC 
ACAGATTCCC CCACGTCTCC TACTGCAGAT GCCCCTCCGC TGCCCCCTAG GAACGCAGGC 
AAAGGTAATG ACTTGGGCCC GAGTGCTGCA GACAGAACTC TAAATAAGTT TGAGAGATTG 
TCACAGTCAT CAGGCACAGG AACAACAAAG ACTGCATTAG GCCCAAGAGT CCTACCAAAA 
CTACCTCAGA AAGTGGCACT TAGGAAAATA GACACCACTC ATCATCTCTC AATGGATAAG 
TATAACCAGC AACCCGAAAT CTTCCAGAAA TCTCCCCAGC CTGGAGATCT GCCTCAGAAG 
CCACAAATTG CAGATCTTCC TCCGAAACCG CAGCCTTTGG ATATCGCCCA GAAACCACAC 
GTCGGAGATC TACCTCCCAA GCCCGGAGAA TTGCCTCCTA AACCTCAGCT AGGAGATTTG 
CCCCCGAAAC CGCAAGATTT ACCTCCAAAG CCACAGCTGA AAGATCTTCC GCCCAAGCCC 
AACCTGGCAG AGGTGATGTG CAAAACTCCT GTTGCGGATA TAGTCCCCAA ACCTCATACC 
AATGAGGCCA ATCAAAATTC CCAGCCGGTG GATGCGACCC ATAATGCACA GCCCACGGAC 
CAAACCCCTG TCCAGAAGCA AGTTCCAGAA ACTTCCAATA ATGGCCCACC GCCTCTTCCA 
GAAACACCCG TGCCACTTCC CCGAAAGATA AACACAGGGA AGAATAAGCT TAAACGAGTT 
AAAACAATAT ACGACTGCCA AGCGGACAAT GATGATGAAC TCACATTTGC GGAGGGGGAA 
GTGATTATCG TCACTGGAGA GGAGGACCAG GAGTGGTGGA TTGGGCACAT CGAAGGCTTC 
CCGGACAGGA AAGGAGTATT CCCTGTGTCC TTCGTCCACC TGTTGACTGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819592.1
CDS	498..3671
Translation	MMPDQISVSEFISETTEDYNSPTTSSFTTRLQSCRNSINVLEEALDQDRTALQKVKKSVKAIYNSGQDHVQNEENYAQALDKFGSNFLSRDNADLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGVKGDLKKPFDRAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDQKEDSQSKQAGYSMHQLQGNKEYGSEKKGYLLKKSDGIRKVWQRRKCTVKNGILTISHATSNRPPTKLNLLTCQVKPNADEKKCFDLISHNRTYHFQAEDEQEYIAWISVLTNSKEDALNMAFRGDQSTGESSLEDLTKAIIDDVQKTPGNEVCCDCGSPDPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDKLGTSELLLAKNVGNNSFNDIMEGNLLSPSAKPSPSSDMIARKEYITAKYVERRFSRKICSTGAEKLNELLEAVKSRDLLALIQVYAEGVEIMEPLLESGQEPGETALHYAVRTADHTSLPLVDFLVQNCGNLDKQTGKGNTALHYCCLYNKPECLKLLLRGKPTIDAVNHIGETAFDVAKRQKAAQCEELVSQALAGKLNPHVHVEYDWNLRQEEMDESDDDLEDKPSPIKKERSPRPQSFCHSSSISPQDKLSLPGFNTPRDKQRLSYGAFTNQIFMSTSTDSPTSPTADAPPLPPRNAGKGNDLGPSAADRTLNKFERLSQSSGTGTTKTALGPRVLPKLPQKVALRKIDTTHHLSMDKYNQQPEIFQKSPQPGDLPQKPQIADLPPKPQPLDIAQKPHVGDLPPKPGELPPKPQLGDLPPKPQDLPPKPQLKDLPPKPNLAEVMCKTPVADIVPKPHTNEANQNSQPVDATHNAQPTDQTPVQKQVPETSNNGPPPLPETPVPLPRKINTGKNKLKRVKTIYDCQADNDDELTFAEGEVIIVTGEEDQEWWIGHIEGFPDRKGVFPVSFVHLLTE

Target ORF information:

RefSeq Version	XM_012964138.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (asap1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012964138.2

ORF Insert Sequence:

ATGATGCCCG ATCAGATCTC AGTATCGGAG TTTATATCGG AGACCACAGA GGATTACAAT 
TCGCCAACCA CGTCCAGTTT CACGACCCGT CTTCAGAGCT GCAGGAATTC GATCAATGTG 
CTGGAAGAGG CCTTAGATCA AGATAGGACA GCGTTGCAGA AAGTGAAAAA ATCGGTGAAA 
GCAATTTATA ATTCAGGCCA AGATCATGTC CAAAATGAAG AAAACTATGC ACAAGCCTTA 
GATAAATTTG GAAGTAATTT TCTAAGTCGG GATAACGCAG ATTTGGGCAC CGCCTTCGTG 
AAATTCTCCA CTCTAACAAA AGAACTATCA ACGCTTCTTA AAAACCTGCT ACAGGGATTA 
AGCCACAATG TCATCTTCAC CTTGGATTCT CTCTTGAAAG GGGACTTAAA GGGCGTAAAA 
GGGGACCTTA AGAAGCCATT CGACAGAGCG TGGAAGGATT ATGAAACCAA ATTCACGAAA 
ATCGAAAAGG AGAAAAGGGA ACACGCCAAG CAACATGGGA TGATCCGTAC GGAGATTACT 
GGGGCCGAGA TTGCCGAAGA GATGGAGAAG GAGAGGAGAT TATTCCAGCT ACAGATGTGT 
GAGTATCTCA TTAAAGTTAA TGAAATCAAG ACTAAGAAAG GCGTGGATCT GCTGCAGAAC 
CTTATAAAGT ACTATCACGC ACAGTGCAAT TTCTTCCAGG ATGGATTGAA AACAGCTGAC 
AAGCTGAAGC AGTACATAGA GAAATTGGCT GCAGATTTGT ACAATATTAA GCAGACCCAA 
GATGAGGAGA AGAAACAGCT CACTGCCCTG CGAGACCTGA TAAAATCCTC TTTACAGCTG 
GATCAGAAGG AGGATTCCCA GAGTAAGCAG GCAGGCTACA GCATGCACCA GCTCCAGGGG 
AACAAGGAGT ATGGCAGCGA GAAGAAAGGC TACCTGCTGA AAAAAAGTGA TGGCATCCGG 
AAAGTATGGC AGAGGAGGAA GTGCACTGTG AAAAATGGAA TCCTGACCAT TTCCCATGCA 
ACGTCAAACA GGCCACCGAC GAAGCTGAAC CTGCTGACCT GCCAAGTCAA GCCGAACGCT 
GATGAGAAGA AATGCTTTGA TCTCATCTCA CACAACAGAA CATACCACTT CCAAGCAGAG 
GACGAACAAG AATACATTGC GTGGATATCA GTTCTGACAA ATAGCAAAGA AGATGCACTC 
AATATGGCTT TCCGGGGAGA CCAGAGCACT GGCGAGAGCA GCCTGGAGGA TCTGACTAAG 
GCCATTATAG ACGATGTACA GAAAACGCCT GGAAATGAAG TTTGCTGTGA TTGTGGGTCA 
CCGGACCCGA CATGGCTTTC CACAAACCTT GGGATTTTAA CCTGTATAGA ATGTTCGGGC 
ATACATAGGG AAATGGGGGT TCATATCTCT AGAATCCAAT CTCTGGAATT AGACAAATTA 
GGGACTTCTG AACTCTTGCT GGCCAAGAAT GTAGGAAACA ATAGCTTCAA TGATATTATG 
GAAGGGAATT TGCTGTCTCC GTCAGCCAAG CCTTCTCCCT CCAGTGATAT GATTGCACGT 
AAAGAATATA TCACTGCCAA GTACGTGGAG CGGAGGTTTT CCAGAAAGAT CTGTTCTACT 
GGGGCAGAAA AACTGAATGA GCTACTTGAG GCTGTGAAAT CCAGGGACTT ACTTGCACTA 
ATCCAAGTCT ATGCAGAAGG TGTAGAGATC ATGGAACCAC TGCTAGAATC TGGACAGGAG 
CCAGGTGAAA CTGCACTTCA TTATGCAGTC CGGACAGCTG ACCACACGTC TCTTCCCTTG 
GTGGACTTCC TGGTACAAAA CTGTGGAAAT TTAGATAAGC AGACTGGAAA GGGCAACACT 
GCTTTACACT ACTGCTGCTT ATATAACAAG CCGGAATGCT TAAAATTGCT TTTGAGGGGA 
AAGCCAACTA TTGATGCTGT TAACCATATT GGTGAGACGG CTTTTGATGT AGCAAAGAGA 
CAGAAAGCAG CCCAGTGTGA GGAACTGGTA TCCCAAGCCC TGGCAGGGAA GCTGAATCCT 
CATGTTCACG TGGAGTACGA CTGGAACCTG CGTCAGGAAG AAATGGATGA GAGCGACGAT 
GATTTGGAAG ATAAGCCAAG CCCAATAAAG AAAGAGCGTT CCCCCAGACC CCAGAGCTTC 
TGCCATTCGT CCAGCATTTC CCCACAAGAC AAGCTGTCGC TGCCAGGTTT CAATACTCCT 
CGGGACAAGC AGAGACTCTC TTATGGCGCC TTTACCAACC AAATATTCAT GTCTACAAGC 
ACAGATTCCC CCACGTCTCC TACTGCAGAT GCCCCTCCGC TGCCCCCTAG GAACGCAGGC 
AAAGGTAATG ACTTGGGCCC GAGTGCTGCA GACAGAACTC TAAATAAGTT TGAGAGATTG 
TCACAGTCAT CAGGCACAGG AACAACAAAG ACTGCATTAG GCCCAAGAGT CCTACCAAAA 
CTACCTCAGA AAGTGGCACT TAGGAAAATA GACACCACTC ATCATCTCTC AATGGATAAG 
TATAACCAGC AACCCGAAAT CTTCCAGAAA TCTCCCCAGC CTGGAGATCT GCCTCAGAAG 
CCACAAATTG CAGATCTTCC TCCGAAACCG CAGCCTTTGG ATATCGCCCA GAAACCACAC 
GTCGGAGATC TACCTCCCAA GCCCGGAGAA TTGCCTCCTA AACCTCAGCT AGGAGATTTG 
CCCCCGAAAC CGCAAGATTT ACCTCCAAAG CCACAGCTGA AAGATCTTCC GCCCAAGCCC 
AACCTGGCAG AGGTGATGTG CAAAACTCCT GTTGCGGATA TAGTCCCCAA ACCTCATACC 
AATGAGGCCA ATCAAAATTC CCAGCCGGTG GATGCGACCC ATAATGCACA GCCCACGGAC 
CAAACCCCTG TCCAGAAGCA AGTTCCAGAA ACTTCCAATA ATGGCCCACC GCCTCTTCCA 
GAAACACCCG TGCCACTTCC CCGAAAGATA AACACAGGGA AGAATAAGCT TAAACGAGTT 
AAAACAATAT ACGACTGCCA AGCGGACAAT GATGATGAAC TCACATTTGC GGAGGGGGAA 
GTGATTATCG TCACTGGAGA GGAGGACCAG GAGTGGTGGA TTGGGCACAT CGAAGGCTTC 
CCGGACAGGA AAGGAGTATT CCCTGTGTCC TTCGTCCACC TGTTGACTGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	asap1
Entrez Gene ID	100036721
Full Name	ArfGAP with SH3 domain, ankyrin repeat and PH domain 1
Synonyms	ddef1
General protein information	Preferred Names ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 Names arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

	ASAP1	XP_001084688.1
Homo sapiens (human)	ASAP1	NP_060952.2
Gallus gallus (chicken)	ASAP1	XP_425945.4
Drosophila melanogaster (fruit fly)	Asap1	NP_001163085.1
Xenopus tropicalis (tropical clawed frog)	asap1	NP_001090735.1
Pan troglodytes (chimpanzee)	ASAP1	XP_003311959.1
Mus musculus (house mouse)	Asap1	NP_034156.2
Danio rerio (zebrafish)	asap1b	XP_005162660.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007726	XP_001687844.1
Canis lupus familiaris (dog)	ASAP1	XP_005627995.1
Bos taurus (cattle)	ASAP1	NP_787015.1
Rattus norvegicus (Norway rat)	Asap1	NP_001037710.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

asap1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

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