asap1b cDNA ORF clone, Danio rerio(zebrafish)

The following asap1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the asap1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa151786 XM_009297309.3
Latest version!
Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 1b (asap1b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa151787 XM_009297310.3
Latest version!
Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 1b (asap1b), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa151788 XM_021470289.1
Latest version!
Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 1b (asap1b), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa151789 XM_005162605.4
Latest version!
Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 1b (asap1b), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa151790 XM_005162606.4
Latest version!
Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 1b (asap1b), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa151791 XM_003201277.5
Latest version!
Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 1b (asap1b), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa44735 XM_009297312.3
Latest version!
Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 1b (asap1b), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa57693 XM_017353941.1
Latest version!
Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 1b (asap1b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa57694 XM_017353942.1
Latest version!
Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 1b (asap1b), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa57695 XM_017353943.1
Latest version!
Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 1b (asap1b), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa28402 XM_005162605.3
Latest version!
Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 1b (asap1b), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa28403 XM_005162606.3
Latest version!
Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 1b (asap1b), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa28404 XM_003201277.4
Latest version!
Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 1b (asap1b), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa44731 XM_009297309.2
Latest version!
Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 1b (asap1b), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa44732 XM_009297310.2
Latest version!
Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 1b (asap1b), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa44734 XM_009297312.2
Latest version!
Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 1b (asap1b), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa151786
    Clone ID Related Accession (Same CDS sequence) XM_009297309.3
    Accession Version XM_009297309.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3414bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009297309.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGATGAGGT CATCATCCTC TCGTCTTAGT TTTTCCTCTA AGGAACCCAT ATGGAACAGG 
    ATGCCAGATC AAATATCGGT GTCGGAGTTT CTGTCGGAAA CAACAGAGGA TTACAACTCT
    CCCACTACAT CCAGCTTCAC CACCCGCTTG CAGAGCTGCA GGAATACAGT CAGCGTGTTA
    GAAGAGGCTT TGGATCAGGA CAGAACGTCT TTGCAGAAGG TCAAGAAGTC AGTCAAAGCC
    ATTTACAACT CTGGCCAAGA TCACATTCAA AATGAGGAAA CGTATTCCCA GGCATTGGAT
    AAATTTGGCA GCAACTTCAT CAGTCGGGAT AATCCAGATC TGGGCACAGC CTTTGTTAAA
    TTCTCCACTT TGACTAAAGA GCTTTCTGCC CTCCTCAAGA ACCTGCTTCA GAATTTAAGT
    CATAATGTGA TCTTTACACT GGACTCCCTG TTGAAAGGCG ACCTGAAAGG CGTGAAGGGG
    GACCTGAAAA AACCCTTTGA CAAGGCTTGG AAGGACTACG AAACCAAGTT TACAAAAATC
    GAAAAGGAAA AACGTGAGCA TGCCAAACAG CATGGAATGA TCCGAACAGA GATCACTGGT
    GCTGAGATCG CAGAGGAGAT GGAGAAAGAA AGGAGGCTCT TCCAGCTACA GATGTGTGAG
    TACCTGATCA AAGTAAATGA AATAAAGACC AAGAAAGGAG TTGACCTCCT TCAGAACTTG
    ATCAAGTATT ACCACGCGCA ATGCAACTTT TTCCAAGATG GCTTGAAGAC GGCAGATAAA
    CTGAAGCAGT ACATTGAGAA GTTAGCAGCT GATCTTTACA ATATAAAACA AACACAAGAT
    GAAGAGAAAA AGCAGCTCAC GGCTTTAAGA GATCTCATTA AATCCTCTCT ACAAGTGGAC
    CAAAAGGAGT CTAGAAGACA GGACTCTCAG AGCAAACAGG GTGGCTACAG TATGCACCAG
    CTGCAGGGTA ACAAAGAGTT TGGCTGTGAG AAGAAAGGCT ATCTGTTAAA GAAGAGTGAT
    GGGTTAAGGA AGGTGTGGCA GAGAAGGAAG TGCTCAGTTA AAGGCGGGAT TCTCACCATC
    TCTCACGCCA CTTCAAACAG GCAGCCTGTC AAACTCAACC TGCTCACATG CCAGGTCAAG
    CCCAGTGCTG AGGACAGAAA GTGCTTCGAT CTTATTTCCC ATAACCGCAC ATATCACTTT
    CAGGCAGAAG ATGAGCAGGA ATTTATAATA TGGATCTCAG TGTTAACTAA CAGCAAGGAG
    GAGGCACTTA ACATGGCCTT CAGAGGAGAG CAGGGTGGAG GAGATGATGG GCTGGAGGAT
    CTCACCAAAG CCATAATCGA TGATGTGCTG CGCATGCCGG GAAATGAGTG GTGCTGTGAT
    TGTGGGGCTC CAGATCCCAA ATGGTTGTCG ACTAACCTGG GAATCCTGAC ATGCATCGAA
    TGTTCAGGCA TACACCGGGA GATGGGCGTC CACATCTCAC GCATCCAGTC TATGGAGCTA
    GACAAACTGG GAACCTCCGA ACTCTTGCTG GCCAAGAATG TGGGGAACAG TAGTTTTAAT
    GATATAATGG AGGGAGGTCT TTCTTGTCCC TCTTCTAAAC CCACTCCCTC TAGTGACATG
    ACTGTTCGTA AGGAGTTCAT CAATGCTAAA TATGTAGATC ATAAGTTTGC AAGGAAAACC
    TGCAGCTCAG CAGAAGACAA GAGGAGTGAT CTTTTTAAAG CCGTCGGGTC ACATGACCTG
    CTAGCTCTCA TCCAGGTGTA TGCTGAGGGG GTAGAGCTTT TGGAACCACT ACATGAAGGA
    GGACAGGAAG GTGGAGAGAC TGCATTGCAC TATGCCGTGA GGACAGCTGA CCAAACCTCA
    CTGCACCTGG TCGACTTCCT TGTCCAGAAC AGTGGGAATT TGGATAAGCA GACGGAGCGA
    GGAAACACGG CCCTGCACTA TTGCTGCCTG TACGAGAAAC ATGAATGCCT CAAACTACTG
    CTCCGAGGCA AACCAGCTAC TGATATCACC AATCAGAATG GGGACACAGC TTTGGATGTG
    GCTAGGCGGA TGAAGCTCTT CCAGTGTGAG GAAGCATTGG TACAAGCTGC TGCTGGAAAA
    TTTAATACTA AAGTCCATGT GGAATATGAA TGGAATCTCA GGTTGGAGGA GCTTGATGAA
    AGTGATGATG ATCTGGATTA CAAACCAAGT CCCATTAAGA AGGATCGCTC ACCCCGTCCG
    CAGAGTTTCT GCCACTCATC CAGCCTCTCC CCTCATGATA AGCTCTCTCT GCCTGCTTTC
    ATTGGTCAGC GTGATAAGCA ACGCCTCTCC TACTCTGCAT TCACCAATCA GATGTACAGC
    GTTTCCACCG ACTGTCCGTC CTCTCCGGTT TCAGATGCTC CACCTCTGCC GCCAAGGAAT
    GCTGGCAAAG CCCCTCCATT GTCGAACCTG CCTCCCTCCA CCCTTGCCCC TGCATCACAG
    ACCTCACCTA GTGGCAGCTC TACCCTGTCA AAGAAGAGAC CCCCTCCCCC TCCCCCTGGA
    CACAAACGCA CGCTATCTGA CCCCCCTACC CCTCTCTTTA AAGGAAGTGA TTCAACGCCG
    CCTCCTGTCA ACAAGATGTC CCCTGTAACT AATAGATTTG AGGGCATCCC ACAACAGCAA
    AGTCCCAACA CCACCAAGCC CACACTTGGT CCAAGGGTCC TTCCCAAACT ACCTCAGAAG
    GTTGCCCTGC GTAAGATAGA CACCATACAT CACCCATCTG TGGACAAAAC CAACCAGCCT
    TCTGAAGCTG TCCTTTTTCA GAAAGTTCTG CAGAGTTCAG ATACATCCCA GAAAGTTCCG
    ATAGCTGACA GGCCTCAGCC TGGAGACGTC GCTCCACAGC GACCACAGCC TTCAGAATTA
    CCACCTAAAC CAGGAGAACT GCCTCCAAAG CCTCAACTGT CCGAACTTCC CCCTAAACCC
    CAGTTGGGTG ACCTACCACC TAAACCTCAG CTTAAAGATC TCCCGCCCAA GCCCCAACTC
    GCAGACATCT CCCCAGCCAA ACCCATAACC ATTGAGGTCC CCCCAAAACC TTCTCCAGGA
    GAAGTGGCTC CCAAGCCCCA ACCTCCAGAT ACTCCAACAA TCATTCAACA AGGTGAACTG
    TCACCTCAGC CTGTGATCCA GTCTAGTGAA GATACCAATG GAAGTCCAAC ATCTGCACAG
    GACACACCCG TACCATTGCC CAGAAAAATA AATCCGATTA AGAGTAAAAT GCGAAGGGTG
    AAGACTATTT ATGATTGCCA GGCAGACAAT GATGATGAGC TGACTTTCGC TGAGGGTGAG
    GTCATTGTGG TAACTGGTGA GGAAGATCAG GAGTGGTGGA TTGGGCACAT CGAAGGACAG
    CCGGAGAGAA AAGGTGTATT TCCCATGTCC TTTGTTCATA TCTTATCAGA TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295584.2
    CDS170..3583
    Translation

    Target ORF information:

    RefSeq Version XM_009297309.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 1b (asap1b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009297309.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGATGAGGT CATCATCCTC TCGTCTTAGT TTTTCCTCTA AGGAACCCAT ATGGAACAGG 
    ATGCCAGATC AAATATCGGT GTCGGAGTTT CTGTCGGAAA CAACAGAGGA TTACAACTCT
    CCCACTACAT CCAGCTTCAC CACCCGCTTG CAGAGCTGCA GGAATACAGT CAGCGTGTTA
    GAAGAGGCTT TGGATCAGGA CAGAACGTCT TTGCAGAAGG TCAAGAAGTC AGTCAAAGCC
    ATTTACAACT CTGGCCAAGA TCACATTCAA AATGAGGAAA CGTATTCCCA GGCATTGGAT
    AAATTTGGCA GCAACTTCAT CAGTCGGGAT AATCCAGATC TGGGCACAGC CTTTGTTAAA
    TTCTCCACTT TGACTAAAGA GCTTTCTGCC CTCCTCAAGA ACCTGCTTCA GAATTTAAGT
    CATAATGTGA TCTTTACACT GGACTCCCTG TTGAAAGGCG ACCTGAAAGG CGTGAAGGGG
    GACCTGAAAA AACCCTTTGA CAAGGCTTGG AAGGACTACG AAACCAAGTT TACAAAAATC
    GAAAAGGAAA AACGTGAGCA TGCCAAACAG CATGGAATGA TCCGAACAGA GATCACTGGT
    GCTGAGATCG CAGAGGAGAT GGAGAAAGAA AGGAGGCTCT TCCAGCTACA GATGTGTGAG
    TACCTGATCA AAGTAAATGA AATAAAGACC AAGAAAGGAG TTGACCTCCT TCAGAACTTG
    ATCAAGTATT ACCACGCGCA ATGCAACTTT TTCCAAGATG GCTTGAAGAC GGCAGATAAA
    CTGAAGCAGT ACATTGAGAA GTTAGCAGCT GATCTTTACA ATATAAAACA AACACAAGAT
    GAAGAGAAAA AGCAGCTCAC GGCTTTAAGA GATCTCATTA AATCCTCTCT ACAAGTGGAC
    CAAAAGGAGT CTAGAAGACA GGACTCTCAG AGCAAACAGG GTGGCTACAG TATGCACCAG
    CTGCAGGGTA ACAAAGAGTT TGGCTGTGAG AAGAAAGGCT ATCTGTTAAA GAAGAGTGAT
    GGGTTAAGGA AGGTGTGGCA GAGAAGGAAG TGCTCAGTTA AAGGCGGGAT TCTCACCATC
    TCTCACGCCA CTTCAAACAG GCAGCCTGTC AAACTCAACC TGCTCACATG CCAGGTCAAG
    CCCAGTGCTG AGGACAGAAA GTGCTTCGAT CTTATTTCCC ATAACCGCAC ATATCACTTT
    CAGGCAGAAG ATGAGCAGGA ATTTATAATA TGGATCTCAG TGTTAACTAA CAGCAAGGAG
    GAGGCACTTA ACATGGCCTT CAGAGGAGAG CAGGGTGGAG GAGATGATGG GCTGGAGGAT
    CTCACCAAAG CCATAATCGA TGATGTGCTG CGCATGCCGG GAAATGAGTG GTGCTGTGAT
    TGTGGGGCTC CAGATCCCAA ATGGTTGTCG ACTAACCTGG GAATCCTGAC ATGCATCGAA
    TGTTCAGGCA TACACCGGGA GATGGGCGTC CACATCTCAC GCATCCAGTC TATGGAGCTA
    GACAAACTGG GAACCTCCGA ACTCTTGCTG GCCAAGAATG TGGGGAACAG TAGTTTTAAT
    GATATAATGG AGGGAGGTCT TTCTTGTCCC TCTTCTAAAC CCACTCCCTC TAGTGACATG
    ACTGTTCGTA AGGAGTTCAT CAATGCTAAA TATGTAGATC ATAAGTTTGC AAGGAAAACC
    TGCAGCTCAG CAGAAGACAA GAGGAGTGAT CTTTTTAAAG CCGTCGGGTC ACATGACCTG
    CTAGCTCTCA TCCAGGTGTA TGCTGAGGGG GTAGAGCTTT TGGAACCACT ACATGAAGGA
    GGACAGGAAG GTGGAGAGAC TGCATTGCAC TATGCCGTGA GGACAGCTGA CCAAACCTCA
    CTGCACCTGG TCGACTTCCT TGTCCAGAAC AGTGGGAATT TGGATAAGCA GACGGAGCGA
    GGAAACACGG CCCTGCACTA TTGCTGCCTG TACGAGAAAC ATGAATGCCT CAAACTACTG
    CTCCGAGGCA AACCAGCTAC TGATATCACC AATCAGAATG GGGACACAGC TTTGGATGTG
    GCTAGGCGGA TGAAGCTCTT CCAGTGTGAG GAAGCATTGG TACAAGCTGC TGCTGGAAAA
    TTTAATACTA AAGTCCATGT GGAATATGAA TGGAATCTCA GGTTGGAGGA GCTTGATGAA
    AGTGATGATG ATCTGGATTA CAAACCAAGT CCCATTAAGA AGGATCGCTC ACCCCGTCCG
    CAGAGTTTCT GCCACTCATC CAGCCTCTCC CCTCATGATA AGCTCTCTCT GCCTGCTTTC
    ATTGGTCAGC GTGATAAGCA ACGCCTCTCC TACTCTGCAT TCACCAATCA GATGTACAGC
    GTTTCCACCG ACTGTCCGTC CTCTCCGGTT TCAGATGCTC CACCTCTGCC GCCAAGGAAT
    GCTGGCAAAG CCCCTCCATT GTCGAACCTG CCTCCCTCCA CCCTTGCCCC TGCATCACAG
    ACCTCACCTA GTGGCAGCTC TACCCTGTCA AAGAAGAGAC CCCCTCCCCC TCCCCCTGGA
    CACAAACGCA CGCTATCTGA CCCCCCTACC CCTCTCTTTA AAGGAAGTGA TTCAACGCCG
    CCTCCTGTCA ACAAGATGTC CCCTGTAACT AATAGATTTG AGGGCATCCC ACAACAGCAA
    AGTCCCAACA CCACCAAGCC CACACTTGGT CCAAGGGTCC TTCCCAAACT ACCTCAGAAG
    GTTGCCCTGC GTAAGATAGA CACCATACAT CACCCATCTG TGGACAAAAC CAACCAGCCT
    TCTGAAGCTG TCCTTTTTCA GAAAGTTCTG CAGAGTTCAG ATACATCCCA GAAAGTTCCG
    ATAGCTGACA GGCCTCAGCC TGGAGACGTC GCTCCACAGC GACCACAGCC TTCAGAATTA
    CCACCTAAAC CAGGAGAACT GCCTCCAAAG CCTCAACTGT CCGAACTTCC CCCTAAACCC
    CAGTTGGGTG ACCTACCACC TAAACCTCAG CTTAAAGATC TCCCGCCCAA GCCCCAACTC
    GCAGACATCT CCCCAGCCAA ACCCATAACC ATTGAGGTCC CCCCAAAACC TTCTCCAGGA
    GAAGTGGCTC CCAAGCCCCA ACCTCCAGAT ACTCCAACAA TCATTCAACA AGGTGAACTG
    TCACCTCAGC CTGTGATCCA GTCTAGTGAA GATACCAATG GAAGTCCAAC ATCTGCACAG
    GACACACCCG TACCATTGCC CAGAAAAATA AATCCGATTA AGAGTAAAAT GCGAAGGGTG
    AAGACTATTT ATGATTGCCA GGCAGACAAT GATGATGAGC TGACTTTCGC TGAGGGTGAG
    GTCATTGTGG TAACTGGTGA GGAAGATCAG GAGTGGTGGA TTGGGCACAT CGAAGGACAG
    CCGGAGAGAA AAGGTGTATT TCCCATGTCC TTTGTTCATA TCTTATCAGA TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa151787
    Clone ID Related Accession (Same CDS sequence) XM_009297310.3
    Accession Version XM_009297310.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3411bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009297310.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGATGAGGT CATCATCCTC TCGTCTTAGT TTTTCCTCTA AGGAACCCAT ATGGAACAGG 
    ATGCCAGATC AAATATCGGT GTCGGAGTTT CTGTCGGAAA CAACAGAGGA TTACAACTCT
    CCCACTACAT CCAGCTTCAC CACCCGCTTG CAGAGCTGCA GGAATACAGT CAGCGTGTTA
    GAAGAGGCTT TGGATCAGGA CAGAACGTCT TTGCAGAAGG TCAAGAAGTC AGTCAAAGCC
    ATTTACAACT CTGGCCAAGA TCACATTCAA AATGAGGAAA CGTATTCCCA GGCATTGGAT
    AAATTTGGCA GCAACTTCAT CAGTCGGGAT AATCCAGATC TGGGCACAGC CTTTGTTAAA
    TTCTCCACTT TGACTAAAGA GCTTTCTGCC CTCCTCAAGA ACCTGCTTCA GAATTTAAGT
    CATAATGTGA TCTTTACACT GGACTCCCTG TTGAAAGGCG ACCTGAAAGG CGTGAAGGGG
    GACCTGAAAA AACCCTTTGA CAAGGCTTGG AAGGACTACG AAACCAAGTT TACAAAAATC
    GAAAAGGAAA AACGTGAGCA TGCCAAACAG CATGGAATGA TCCGAACAGA GATCACTGGT
    GCTGAGATCG CAGAGGAGAT GGAGAAAGAA AGGAGGCTCT TCCAGCTACA GATGTGTGAG
    TACCTGATCA AAGTAAATGA AATAAAGACC AAGAAAGGAG TTGACCTCCT TCAGAACTTG
    ATCAAGTATT ACCACGCGCA ATGCAACTTT TTCCAAGATG GCTTGAAGAC GGCAGATAAA
    CTGAAGCAGT ACATTGAGAA GTTAGCAGCT GATCTTTACA ATATAAAACA AACACAAGAT
    GAAGAGAAAA AGCAGCTCAC GGCTTTAAGA GATCTCATTA AATCCTCTCT ACAAGTGGAC
    CAAAAGGAGT CTAGAAGAGA CTCTCAGAGC AAACAGGGTG GCTACAGTAT GCACCAGCTG
    CAGGGTAACA AAGAGTTTGG CTGTGAGAAG AAAGGCTATC TGTTAAAGAA GAGTGATGGG
    TTAAGGAAGG TGTGGCAGAG AAGGAAGTGC TCAGTTAAAG GCGGGATTCT CACCATCTCT
    CACGCCACTT CAAACAGGCA GCCTGTCAAA CTCAACCTGC TCACATGCCA GGTCAAGCCC
    AGTGCTGAGG ACAGAAAGTG CTTCGATCTT ATTTCCCATA ACCGCACATA TCACTTTCAG
    GCAGAAGATG AGCAGGAATT TATAATATGG ATCTCAGTGT TAACTAACAG CAAGGAGGAG
    GCACTTAACA TGGCCTTCAG AGGAGAGCAG GGTGGAGGAG ATGATGGGCT GGAGGATCTC
    ACCAAAGCCA TAATCGATGA TGTGCTGCGC ATGCCGGGAA ATGAGTGGTG CTGTGATTGT
    GGGGCTCCAG ATCCCAAATG GTTGTCGACT AACCTGGGAA TCCTGACATG CATCGAATGT
    TCAGGCATAC ACCGGGAGAT GGGCGTCCAC ATCTCACGCA TCCAGTCTAT GGAGCTAGAC
    AAACTGGGAA CCTCCGAACT CTTGCTGGCC AAGAATGTGG GGAACAGTAG TTTTAATGAT
    ATAATGGAGG GAGGTCTTTC TTGTCCCTCT TCTAAACCCA CTCCCTCTAG TGACATGACT
    GTTCGTAAGG AGTTCATCAA TGCTAAATAT GTAGATCATA AGTTTGCAAG GAAAACCTGC
    AGCTCAGCAG AAGACAAGAG GAGTGATCTT TTTAAAGCCG TCGGGTCACA TGACCTGCTA
    GCTCTCATCC AGGTGTATGC TGAGGGGGTA GAGCTTTTGG AACCACTACA TGAAGGAGGA
    CAGGAAGGTG GAGAGACTGC ATTGCACTAT GCCGTGAGGA CAGCTGACCA AACCTCACTG
    CACCTGGTCG ACTTCCTTGT CCAGAACAGT GGGAATTTGG ATAAGCAGAC GGAGCGAGGA
    AACACGGCCC TGCACTATTG CTGCCTGTAC GAGAAACATG AATGCCTCAA ACTACTGCTC
    CGAGGCAAAC CAGCTACTGA TATCACCAAT CAGAATGGGG ACACAGCTTT GGATGTGGCT
    AGGCGGATGA AGCTCTTCCA GTGTGAGGAA GCATTGGTAC AAGCTGCTGC TGGAAAATTT
    AATACTAAAG TCCATGTGGA ATATGAATGG AATCTCAGGT TGGAGGAGCT TGATGAAAGT
    GATGATGATC TGGATTACAA ACCAAGTCCC ATTAAGAAGG ATCGCTCACC CCGTCCGCAG
    AGTTTCTGCC ACTCATCCAG CCTCTCCCCT CATGATAAGC TCTCTCTGCC TGCTTTCATT
    GGTCAGCGTG ATAAGCAACG CCTCTCCTAC TCTGCATTCA CCAATCAGAT GTACAGCGTT
    TCCACCGACT GTCCGTCCTC TCCGGTTTCA GATGCTCCAC CTCTGCCGCC AAGGAATGCT
    GGCAAAGCCC CTCCATTGTC GAACCTGCCT CCCTCCACCC TTGCCCCTGC ATCACAGACC
    TCACCTAGTG GCAGCTCTAC CCTGTCAAAG AAGAGACCCC CTCCCCCTCC CCCTGGACAC
    AAACGCACGC TATCTGACCC CCCTACCCCT CTCTTTAAAG GAAGTGATTC AACGCCGCCT
    CCTGTCAACA AGATGTCCCC TGTAACTAAT AGATTTGAGG GCATCCCACA ACAGCAAAGT
    CCCAACACCA CCAAGCCCAC ACTTGGTCCA AGGGTCCTTC CCAAACTACC TCAGAAGGTT
    GCCCTGCGTA AGATAGACAC CATACATCAC CCATCTGTGG ACAAAACCAA CCAGCCTTCT
    GAAGCTGTCC TTTTTCAGAA AGTTCTGCAG AGTTCAGATA CATCCCAGAA AGTTCCGATA
    GCTGACAGGC CTCAGCCTGG AGACGTCGCT CCACAGCGAC CACAGCCTTC AGAATTACCA
    CCTAAACCAG GAGAACTGCC TCCAAAGCCT CAACTGTCCG AACTTCCCCC TAAACCCCAG
    TTGGGTGACC TACCACCTAA ACCTCAGCTT AAAGATCTCC CGCCCAAGCC CCAACTCGCA
    GACATCTCCC CAGCCAAACC CATAACCATT GAGGTCCCCC CAAAACCTTC TCCAGGAGAA
    GTGGCTCCCA AGCCCCAACC TCCAGATACT CCAACAATCA TTCAACAAGG TGAACTGTCA
    CCTCAGCCTG TGATCCAGTC TAGTGAAGAT ACCAATGGAA GTCCAACATC TGCACAGGAC
    ACACCCGTAC CATTGCCCAG AAAAATAAAT CCGATTAAGA GTAAAATGCG AAGGGTGAAG
    ACTATTTATG ATTGCCAGGC AGACAATGAT GATGAGCTGA CTTTCGCTGA GGGTGAGGTC
    ATTGTGGTAA CTGGTGAGGA AGATCAGGAG TGGTGGATTG GGCACATCGA AGGACAGCCG
    GAGAGAAAAG GTGTATTTCC CATGTCCTTT GTTCATATCT TATCAGATTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295585.2
    CDS170..3580
    Translation

    Target ORF information:

    RefSeq Version XM_009297310.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 1b (asap1b), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009297310.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGATGAGGT CATCATCCTC TCGTCTTAGT TTTTCCTCTA AGGAACCCAT ATGGAACAGG 
    ATGCCAGATC AAATATCGGT GTCGGAGTTT CTGTCGGAAA CAACAGAGGA TTACAACTCT
    CCCACTACAT CCAGCTTCAC CACCCGCTTG CAGAGCTGCA GGAATACAGT CAGCGTGTTA
    GAAGAGGCTT TGGATCAGGA CAGAACGTCT TTGCAGAAGG TCAAGAAGTC AGTCAAAGCC
    ATTTACAACT CTGGCCAAGA TCACATTCAA AATGAGGAAA CGTATTCCCA GGCATTGGAT
    AAATTTGGCA GCAACTTCAT CAGTCGGGAT AATCCAGATC TGGGCACAGC CTTTGTTAAA
    TTCTCCACTT TGACTAAAGA GCTTTCTGCC CTCCTCAAGA ACCTGCTTCA GAATTTAAGT
    CATAATGTGA TCTTTACACT GGACTCCCTG TTGAAAGGCG ACCTGAAAGG CGTGAAGGGG
    GACCTGAAAA AACCCTTTGA CAAGGCTTGG AAGGACTACG AAACCAAGTT TACAAAAATC
    GAAAAGGAAA AACGTGAGCA TGCCAAACAG CATGGAATGA TCCGAACAGA GATCACTGGT
    GCTGAGATCG CAGAGGAGAT GGAGAAAGAA AGGAGGCTCT TCCAGCTACA GATGTGTGAG
    TACCTGATCA AAGTAAATGA AATAAAGACC AAGAAAGGAG TTGACCTCCT TCAGAACTTG
    ATCAAGTATT ACCACGCGCA ATGCAACTTT TTCCAAGATG GCTTGAAGAC GGCAGATAAA
    CTGAAGCAGT ACATTGAGAA GTTAGCAGCT GATCTTTACA ATATAAAACA AACACAAGAT
    GAAGAGAAAA AGCAGCTCAC GGCTTTAAGA GATCTCATTA AATCCTCTCT ACAAGTGGAC
    CAAAAGGAGT CTAGAAGAGA CTCTCAGAGC AAACAGGGTG GCTACAGTAT GCACCAGCTG
    CAGGGTAACA AAGAGTTTGG CTGTGAGAAG AAAGGCTATC TGTTAAAGAA GAGTGATGGG
    TTAAGGAAGG TGTGGCAGAG AAGGAAGTGC TCAGTTAAAG GCGGGATTCT CACCATCTCT
    CACGCCACTT CAAACAGGCA GCCTGTCAAA CTCAACCTGC TCACATGCCA GGTCAAGCCC
    AGTGCTGAGG ACAGAAAGTG CTTCGATCTT ATTTCCCATA ACCGCACATA TCACTTTCAG
    GCAGAAGATG AGCAGGAATT TATAATATGG ATCTCAGTGT TAACTAACAG CAAGGAGGAG
    GCACTTAACA TGGCCTTCAG AGGAGAGCAG GGTGGAGGAG ATGATGGGCT GGAGGATCTC
    ACCAAAGCCA TAATCGATGA TGTGCTGCGC ATGCCGGGAA ATGAGTGGTG CTGTGATTGT
    GGGGCTCCAG ATCCCAAATG GTTGTCGACT AACCTGGGAA TCCTGACATG CATCGAATGT
    TCAGGCATAC ACCGGGAGAT GGGCGTCCAC ATCTCACGCA TCCAGTCTAT GGAGCTAGAC
    AAACTGGGAA CCTCCGAACT CTTGCTGGCC AAGAATGTGG GGAACAGTAG TTTTAATGAT
    ATAATGGAGG GAGGTCTTTC TTGTCCCTCT TCTAAACCCA CTCCCTCTAG TGACATGACT
    GTTCGTAAGG AGTTCATCAA TGCTAAATAT GTAGATCATA AGTTTGCAAG GAAAACCTGC
    AGCTCAGCAG AAGACAAGAG GAGTGATCTT TTTAAAGCCG TCGGGTCACA TGACCTGCTA
    GCTCTCATCC AGGTGTATGC TGAGGGGGTA GAGCTTTTGG AACCACTACA TGAAGGAGGA
    CAGGAAGGTG GAGAGACTGC ATTGCACTAT GCCGTGAGGA CAGCTGACCA AACCTCACTG
    CACCTGGTCG ACTTCCTTGT CCAGAACAGT GGGAATTTGG ATAAGCAGAC GGAGCGAGGA
    AACACGGCCC TGCACTATTG CTGCCTGTAC GAGAAACATG AATGCCTCAA ACTACTGCTC
    CGAGGCAAAC CAGCTACTGA TATCACCAAT CAGAATGGGG ACACAGCTTT GGATGTGGCT
    AGGCGGATGA AGCTCTTCCA GTGTGAGGAA GCATTGGTAC AAGCTGCTGC TGGAAAATTT
    AATACTAAAG TCCATGTGGA ATATGAATGG AATCTCAGGT TGGAGGAGCT TGATGAAAGT
    GATGATGATC TGGATTACAA ACCAAGTCCC ATTAAGAAGG ATCGCTCACC CCGTCCGCAG
    AGTTTCTGCC ACTCATCCAG CCTCTCCCCT CATGATAAGC TCTCTCTGCC TGCTTTCATT
    GGTCAGCGTG ATAAGCAACG CCTCTCCTAC TCTGCATTCA CCAATCAGAT GTACAGCGTT
    TCCACCGACT GTCCGTCCTC TCCGGTTTCA GATGCTCCAC CTCTGCCGCC AAGGAATGCT
    GGCAAAGCCC CTCCATTGTC GAACCTGCCT CCCTCCACCC TTGCCCCTGC ATCACAGACC
    TCACCTAGTG GCAGCTCTAC CCTGTCAAAG AAGAGACCCC CTCCCCCTCC CCCTGGACAC
    AAACGCACGC TATCTGACCC CCCTACCCCT CTCTTTAAAG GAAGTGATTC AACGCCGCCT
    CCTGTCAACA AGATGTCCCC TGTAACTAAT AGATTTGAGG GCATCCCACA ACAGCAAAGT
    CCCAACACCA CCAAGCCCAC ACTTGGTCCA AGGGTCCTTC CCAAACTACC TCAGAAGGTT
    GCCCTGCGTA AGATAGACAC CATACATCAC CCATCTGTGG ACAAAACCAA CCAGCCTTCT
    GAAGCTGTCC TTTTTCAGAA AGTTCTGCAG AGTTCAGATA CATCCCAGAA AGTTCCGATA
    GCTGACAGGC CTCAGCCTGG AGACGTCGCT CCACAGCGAC CACAGCCTTC AGAATTACCA
    CCTAAACCAG GAGAACTGCC TCCAAAGCCT CAACTGTCCG AACTTCCCCC TAAACCCCAG
    TTGGGTGACC TACCACCTAA ACCTCAGCTT AAAGATCTCC CGCCCAAGCC CCAACTCGCA
    GACATCTCCC CAGCCAAACC CATAACCATT GAGGTCCCCC CAAAACCTTC TCCAGGAGAA
    GTGGCTCCCA AGCCCCAACC TCCAGATACT CCAACAATCA TTCAACAAGG TGAACTGTCA
    CCTCAGCCTG TGATCCAGTC TAGTGAAGAT ACCAATGGAA GTCCAACATC TGCACAGGAC
    ACACCCGTAC CATTGCCCAG AAAAATAAAT CCGATTAAGA GTAAAATGCG AAGGGTGAAG
    ACTATTTATG ATTGCCAGGC AGACAATGAT GATGAGCTGA CTTTCGCTGA GGGTGAGGTC
    ATTGTGGTAA CTGGTGAGGA AGATCAGGAG TGGTGGATTG GGCACATCGA AGGACAGCCG
    GAGAGAAAAG GTGTATTTCC CATGTCCTTT GTTCATATCT TATCAGATTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa151788
    Clone ID Related Accession (Same CDS sequence) XM_021470289.1
    Accession Version XM_021470289.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3405bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGATGAGGT CATCATCCTC TCGTCTTAGT TTTTCCTCTA AGGAACCCAT ATGGAACAGG 
    ATGCCAGATC AAATATCGGT GTCGGAGTTT CTGTCGGAAA CAACAGAGGA TTACAACTCT
    CCCACTACAT CCAGCTTCAC CACCCGCTTG CAGAGCTGCA GGAATACAGT CAGCGTGTTA
    GAAGAGGCTT TGGATCAGGA CAGAACGTCT TTGCAGAAGG TCAAGAAGTC AGTCAAAGCC
    ATTTACAACT CTGGCCAAGA TCACATTCAA AATGAGGAAA CGTATTCCCA GGCATTGGAT
    AAATTTGGCA GCAACTTCAT CAGTCGGGAT AATCCAGATC TGGGCACAGC CTTTGTTAAA
    TTCTCCACTT TGACTAAAGA GCTTTCTGCC CTCCTCAAGA ACCTGCTTCA GAATTTAAGT
    CATAATGTGA TCTTTACACT GGACTCCCTG TTGAAAGGCG ACCTGAAAGG CGTGAAGGGG
    GACCTGAAAA AACCCTTTGA CAAGGCTTGG AAGGACTACG AAACCAAGTT TACAAAAATC
    GAAAAGGAAA AACGTGAGCA TGCCAAACAG CATGGAATGA TCCGAACAGA GATCACTGGT
    GCTGAGATCG CAGAGGAGAT GGAGAAAGAA AGGAGGCTCT TCCAGCTACA GATGTGTGAG
    TACCTGATCA AAGTAAATGA AATAAAGACC AAGAAAGGAG TTGACCTCCT TCAGAACTTG
    ATCAAGTATT ACCACGCGCA ATGCAACTTT TTCCAAGATG GCTTGAAGAC GGCAGATAAA
    CTGAAGCAGT ACATTGAGAA GTTAGCAGCT GATCTTTACA ATATAAAACA AACACAAGAT
    GAAGAGAAAA AGCAGCTCAC GGCTTTAAGA GATCTCATTA AATCCTCTCT ACAAGTGGAC
    CAAAAGGAGC AGGACTCTCA GAGCAAACAG GGTGGCTACA GTATGCACCA GCTGCAGGGT
    AACAAAGAGT TTGGCTGTGA GAAGAAAGGC TATCTGTTAA AGAAGAGTGA TGGGTTAAGG
    AAGGTGTGGC AGAGAAGGAA GTGCTCAGTT AAAGGCGGGA TTCTCACCAT CTCTCACGCC
    ACTTCAAACA GGCAGCCTGT CAAACTCAAC CTGCTCACAT GCCAGGTCAA GCCCAGTGCT
    GAGGACAGAA AGTGCTTCGA TCTTATTTCC CATAACCGCA CATATCACTT TCAGGCAGAA
    GATGAGCAGG AATTTATAAT ATGGATCTCA GTGTTAACTA ACAGCAAGGA GGAGGCACTT
    AACATGGCCT TCAGAGGAGA GCAGGGTGGA GGAGATGATG GGCTGGAGGA TCTCACCAAA
    GCCATAATCG ATGATGTGCT GCGCATGCCG GGAAATGAGT GGTGCTGTGA TTGTGGGGCT
    CCAGATCCCA AATGGTTGTC GACTAACCTG GGAATCCTGA CATGCATCGA ATGTTCAGGC
    ATACACCGGG AGATGGGCGT CCACATCTCA CGCATCCAGT CTATGGAGCT AGACAAACTG
    GGAACCTCCG AACTCTTGCT GGCCAAGAAT GTGGGGAACA GTAGTTTTAA TGATATAATG
    GAGGGAGGTC TTTCTTGTCC CTCTTCTAAA CCCACTCCCT CTAGTGACAT GACTGTTCGT
    AAGGAGTTCA TCAATGCTAA ATATGTAGAT CATAAGTTTG CAAGGAAAAC CTGCAGCTCA
    GCAGAAGACA AGAGGAGTGA TCTTTTTAAA GCCGTCGGGT CACATGACCT GCTAGCTCTC
    ATCCAGGTGT ATGCTGAGGG GGTAGAGCTT TTGGAACCAC TACATGAAGG AGGACAGGAA
    GGTGGAGAGA CTGCATTGCA CTATGCCGTG AGGACAGCTG ACCAAACCTC ACTGCACCTG
    GTCGACTTCC TTGTCCAGAA CAGTGGGAAT TTGGATAAGC AGACGGAGCG AGGAAACACG
    GCCCTGCACT ATTGCTGCCT GTACGAGAAA CATGAATGCC TCAAACTACT GCTCCGAGGC
    AAACCAGCTA CTGATATCAC CAATCAGAAT GGGGACACAG CTTTGGATGT GGCTAGGCGG
    ATGAAGCTCT TCCAGTGTGA GGAAGCATTG GTACAAGCTG CTGCTGGAAA ATTTAATACT
    AAAGTCCATG TGGAATATGA ATGGAATCTC AGGTTGGAGG AGCTTGATGA AAGTGATGAT
    GATCTGGATT ACAAACCAAG TCCCATTAAG AAGGATCGCT CACCCCGTCC GCAGAGTTTC
    TGCCACTCAT CCAGCCTCTC CCCTCATGAT AAGCTCTCTC TGCCTGCTTT CATTGGTCAG
    CGTGATAAGC AACGCCTCTC CTACTCTGCA TTCACCAATC AGATGTACAG CGTTTCCACC
    GACTGTCCGT CCTCTCCGGT TTCAGATGCT CCACCTCTGC CGCCAAGGAA TGCTGGCAAA
    GCCCCTCCAT TGTCGAACCT GCCTCCCTCC ACCCTTGCCC CTGCATCACA GACCTCACCT
    AGTGGCAGCT CTACCCTGTC AAAGAAGAGA CCCCCTCCCC CTCCCCCTGG ACACAAACGC
    ACGCTATCTG ACCCCCCTAC CCCTCTCTTT AAAGGAAGTG ATTCAACGCC GCCTCCTGTC
    AACAAGATGT CCCCTGTAAC TAATAGATTT GAGGGCATCC CACAACAGCA AAGTCCCAAC
    ACCACCAAGC CCACACTTGG TCCAAGGGTC CTTCCCAAAC TACCTCAGAA GGTTGCCCTG
    CGTAAGATAG ACACCATACA TCACCCATCT GTGGACAAAA CCAACCAGCC TTCTGAAGCT
    GTCCTTTTTC AGAAAGTTCT GCAGAGTTCA GATACATCCC AGAAAGTTCC GATAGCTGAC
    AGGCCTCAGC CTGGAGACGT CGCTCCACAG CGACCACAGC CTTCAGAATT ACCACCTAAA
    CCAGGAGAAC TGCCTCCAAA GCCTCAACTG TCCGAACTTC CCCCTAAACC CCAGTTGGGT
    GACCTACCAC CTAAACCTCA GCTTAAAGAT CTCCCGCCCA AGCCCCAACT CGCAGACATC
    TCCCCAGCCA AACCCATAAC CATTGAGGTC CCCCCAAAAC CTTCTCCAGG AGAAGTGGCT
    CCCAAGCCCC AACCTCCAGA TACTCCAACA ATCATTCAAC AAGGTGAACT GTCACCTCAG
    CCTGTGATCC AGTCTAGTGA AGATACCAAT GGAAGTCCAA CATCTGCACA GGACACACCC
    GTACCATTGC CCAGAAAAAT AAATCCGATT AAGAGTAAAA TGCGAAGGGT GAAGACTATT
    TATGATTGCC AGGCAGACAA TGATGATGAG CTGACTTTCG CTGAGGGTGA GGTCATTGTG
    GTAACTGGTG AGGAAGATCA GGAGTGGTGG ATTGGGCACA TCGAAGGACA GCCGGAGAGA
    AAAGGTGTAT TTCCCATGTC CTTTGTTCAT ATCTTATCAG ATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021325964.1
    CDS170..3574
    Translation

    Target ORF information:

    RefSeq Version XM_021470289.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 1b (asap1b), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021470289.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGATGAGGT CATCATCCTC TCGTCTTAGT TTTTCCTCTA AGGAACCCAT ATGGAACAGG 
    ATGCCAGATC AAATATCGGT GTCGGAGTTT CTGTCGGAAA CAACAGAGGA TTACAACTCT
    CCCACTACAT CCAGCTTCAC CACCCGCTTG CAGAGCTGCA GGAATACAGT CAGCGTGTTA
    GAAGAGGCTT TGGATCAGGA CAGAACGTCT TTGCAGAAGG TCAAGAAGTC AGTCAAAGCC
    ATTTACAACT CTGGCCAAGA TCACATTCAA AATGAGGAAA CGTATTCCCA GGCATTGGAT
    AAATTTGGCA GCAACTTCAT CAGTCGGGAT AATCCAGATC TGGGCACAGC CTTTGTTAAA
    TTCTCCACTT TGACTAAAGA GCTTTCTGCC CTCCTCAAGA ACCTGCTTCA GAATTTAAGT
    CATAATGTGA TCTTTACACT GGACTCCCTG TTGAAAGGCG ACCTGAAAGG CGTGAAGGGG
    GACCTGAAAA AACCCTTTGA CAAGGCTTGG AAGGACTACG AAACCAAGTT TACAAAAATC
    GAAAAGGAAA AACGTGAGCA TGCCAAACAG CATGGAATGA TCCGAACAGA GATCACTGGT
    GCTGAGATCG CAGAGGAGAT GGAGAAAGAA AGGAGGCTCT TCCAGCTACA GATGTGTGAG
    TACCTGATCA AAGTAAATGA AATAAAGACC AAGAAAGGAG TTGACCTCCT TCAGAACTTG
    ATCAAGTATT ACCACGCGCA ATGCAACTTT TTCCAAGATG GCTTGAAGAC GGCAGATAAA
    CTGAAGCAGT ACATTGAGAA GTTAGCAGCT GATCTTTACA ATATAAAACA AACACAAGAT
    GAAGAGAAAA AGCAGCTCAC GGCTTTAAGA GATCTCATTA AATCCTCTCT ACAAGTGGAC
    CAAAAGGAGC AGGACTCTCA GAGCAAACAG GGTGGCTACA GTATGCACCA GCTGCAGGGT
    AACAAAGAGT TTGGCTGTGA GAAGAAAGGC TATCTGTTAA AGAAGAGTGA TGGGTTAAGG
    AAGGTGTGGC AGAGAAGGAA GTGCTCAGTT AAAGGCGGGA TTCTCACCAT CTCTCACGCC
    ACTTCAAACA GGCAGCCTGT CAAACTCAAC CTGCTCACAT GCCAGGTCAA GCCCAGTGCT
    GAGGACAGAA AGTGCTTCGA TCTTATTTCC CATAACCGCA CATATCACTT TCAGGCAGAA
    GATGAGCAGG AATTTATAAT ATGGATCTCA GTGTTAACTA ACAGCAAGGA GGAGGCACTT
    AACATGGCCT TCAGAGGAGA GCAGGGTGGA GGAGATGATG GGCTGGAGGA TCTCACCAAA
    GCCATAATCG ATGATGTGCT GCGCATGCCG GGAAATGAGT GGTGCTGTGA TTGTGGGGCT
    CCAGATCCCA AATGGTTGTC GACTAACCTG GGAATCCTGA CATGCATCGA ATGTTCAGGC
    ATACACCGGG AGATGGGCGT CCACATCTCA CGCATCCAGT CTATGGAGCT AGACAAACTG
    GGAACCTCCG AACTCTTGCT GGCCAAGAAT GTGGGGAACA GTAGTTTTAA TGATATAATG
    GAGGGAGGTC TTTCTTGTCC CTCTTCTAAA CCCACTCCCT CTAGTGACAT GACTGTTCGT
    AAGGAGTTCA TCAATGCTAA ATATGTAGAT CATAAGTTTG CAAGGAAAAC CTGCAGCTCA
    GCAGAAGACA AGAGGAGTGA TCTTTTTAAA GCCGTCGGGT CACATGACCT GCTAGCTCTC
    ATCCAGGTGT ATGCTGAGGG GGTAGAGCTT TTGGAACCAC TACATGAAGG AGGACAGGAA
    GGTGGAGAGA CTGCATTGCA CTATGCCGTG AGGACAGCTG ACCAAACCTC ACTGCACCTG
    GTCGACTTCC TTGTCCAGAA CAGTGGGAAT TTGGATAAGC AGACGGAGCG AGGAAACACG
    GCCCTGCACT ATTGCTGCCT GTACGAGAAA CATGAATGCC TCAAACTACT GCTCCGAGGC
    AAACCAGCTA CTGATATCAC CAATCAGAAT GGGGACACAG CTTTGGATGT GGCTAGGCGG
    ATGAAGCTCT TCCAGTGTGA GGAAGCATTG GTACAAGCTG CTGCTGGAAA ATTTAATACT
    AAAGTCCATG TGGAATATGA ATGGAATCTC AGGTTGGAGG AGCTTGATGA AAGTGATGAT
    GATCTGGATT ACAAACCAAG TCCCATTAAG AAGGATCGCT CACCCCGTCC GCAGAGTTTC
    TGCCACTCAT CCAGCCTCTC CCCTCATGAT AAGCTCTCTC TGCCTGCTTT CATTGGTCAG
    CGTGATAAGC AACGCCTCTC CTACTCTGCA TTCACCAATC AGATGTACAG CGTTTCCACC
    GACTGTCCGT CCTCTCCGGT TTCAGATGCT CCACCTCTGC CGCCAAGGAA TGCTGGCAAA
    GCCCCTCCAT TGTCGAACCT GCCTCCCTCC ACCCTTGCCC CTGCATCACA GACCTCACCT
    AGTGGCAGCT CTACCCTGTC AAAGAAGAGA CCCCCTCCCC CTCCCCCTGG ACACAAACGC
    ACGCTATCTG ACCCCCCTAC CCCTCTCTTT AAAGGAAGTG ATTCAACGCC GCCTCCTGTC
    AACAAGATGT CCCCTGTAAC TAATAGATTT GAGGGCATCC CACAACAGCA AAGTCCCAAC
    ACCACCAAGC CCACACTTGG TCCAAGGGTC CTTCCCAAAC TACCTCAGAA GGTTGCCCTG
    CGTAAGATAG ACACCATACA TCACCCATCT GTGGACAAAA CCAACCAGCC TTCTGAAGCT
    GTCCTTTTTC AGAAAGTTCT GCAGAGTTCA GATACATCCC AGAAAGTTCC GATAGCTGAC
    AGGCCTCAGC CTGGAGACGT CGCTCCACAG CGACCACAGC CTTCAGAATT ACCACCTAAA
    CCAGGAGAAC TGCCTCCAAA GCCTCAACTG TCCGAACTTC CCCCTAAACC CCAGTTGGGT
    GACCTACCAC CTAAACCTCA GCTTAAAGAT CTCCCGCCCA AGCCCCAACT CGCAGACATC
    TCCCCAGCCA AACCCATAAC CATTGAGGTC CCCCCAAAAC CTTCTCCAGG AGAAGTGGCT
    CCCAAGCCCC AACCTCCAGA TACTCCAACA ATCATTCAAC AAGGTGAACT GTCACCTCAG
    CCTGTGATCC AGTCTAGTGA AGATACCAAT GGAAGTCCAA CATCTGCACA GGACACACCC
    GTACCATTGC CCAGAAAAAT AAATCCGATT AAGAGTAAAA TGCGAAGGGT GAAGACTATT
    TATGATTGCC AGGCAGACAA TGATGATGAG CTGACTTTCG CTGAGGGTGA GGTCATTGTG
    GTAACTGGTG AGGAAGATCA GGAGTGGTGG ATTGGGCACA TCGAAGGACA GCCGGAGAGA
    AAAGGTGTAT TTCCCATGTC CTTTGTTCAT ATCTTATCAG ATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa151789
    Clone ID Related Accession (Same CDS sequence) XM_005162605.4
    Accession Version XM_005162605.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3330bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005162605.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGATGAGGT CATCATCCTC TCGTCTTAGT TTTTCCTCTA AGGAACCCAT ATGGAACAGG 
    ATGCCAGATC AAATATCGGT GTCGGAGTTT CTGTCGGAAA CAACAGAGGA TTACAACTCT
    CCCACTACAT CCAGCTTCAC CACCCGCTTG CAGAGCTGCA GGAATACAGT CAGCGTGTTA
    GAAGAGGCTT TGGATCAGGA CAGAACGTCT TTGCAGAAGG TCAAGAAGTC AGTCAAAGCC
    ATTTACAACT CTGGCCAAGA TCACATTCAA AATGAGGAAA CGTATTCCCA GGCATTGGAT
    AAATTTGGCA GCAACTTCAT CAGTCGGGAT AATCCAGATC TGGGCACAGC CTTTGTTAAA
    TTCTCCACTT TGACTAAAGA GCTTTCTGCC CTCCTCAAGA ACCTGCTTCA GAATTTAAGT
    CATAATGTGA TCTTTACACT GGACTCCCTG TTGAAAGGCG ACCTGAAAGG CGTGAAGGGG
    GACCTGAAAA AACCCTTTGA CAAGGCTTGG AAGGACTACG AAACCAAGTT TACAAAAATC
    GAAAAGGAAA AACGTGAGCA TGCCAAACAG CATGGAATGA TCCGAACAGA GATCACTGGT
    GCTGAGATCG CAGAGGAGAT GGAGAAAGAA AGGAGGCTCT TCCAGCTACA GATGTGTGAG
    TACCTGATCA AAGTAAATGA AATAAAGACC AAGAAAGGAG TTGACCTCCT TCAGAACTTG
    ATCAAGTATT ACCACGCGCA ATGCAACTTT TTCCAAGATG GCTTGAAGAC GGCAGATAAA
    CTGAAGCAGT ACATTGAGAA GTTAGCAGCT GATCTTTACA ATATAAAACA AACACAAGAT
    GAAGAGAAAA AGCAGCTCAC GGCTTTAAGA GATCTCATTA AATCCTCTCT ACAAGTGGAC
    CAAAAGGAGT CTAGAAGACA GGACTCTCAG AGCAAACAGG GTGGCTACAG TATGCACCAG
    CTGCAGGGTA ACAAAGAGTT TGGCTGTGAG AAGAAAGGCT ATCTGTTAAA GAAGAGTGAT
    GGGTTAAGGA AGGTGTGGCA GAGAAGGAAG TGCTCAGTTA AAGGCGGGAT TCTCACCATC
    TCTCACGCCA CTTCAAACAG GCAGCCTGTC AAACTCAACC TGCTCACATG CCAGGTCAAG
    CCCAGTGCTG AGGACAGAAA GTGCTTCGAT CTTATTTCCC ATAACCGCAC ATATCACTTT
    CAGGCAGAAG ATGAGCAGGA ATTTATAATA TGGATCTCAG TGTTAACTAA CAGCAAGGAG
    GAGGCACTTA ACATGGCCTT CAGAGGAGAG CAGGGTGGAG GAGATGATGG GCTGGAGGAT
    CTCACCAAAG CCATAATCGA TGATGTGCTG CGCATGCCGG GAAATGAGTG GTGCTGTGAT
    TGTGGGGCTC CAGATCCCAA ATGGTTGTCG ACTAACCTGG GAATCCTGAC ATGCATCGAA
    TGTTCAGGCA TACACCGGGA GATGGGCGTC CACATCTCAC GCATCCAGTC TATGGAGCTA
    GACAAACTGG GAACCTCCGA ACTCTTGCTG GCCAAGAATG TGGGGAACAG TAGTTTTAAT
    GATATAATGG AGGGAGGTCT TTCTTGTCCC TCTTCTAAAC CCACTCCCTC TAGTGACATG
    ACTGTTCGTA AGGAGTTCAT CAATGCTAAA TATGTAGATC ATAAGTTTGC AAGGAAAACC
    TGCAGCTCAG CAGAAGACAA GAGGAGTGAT CTTTTTAAAG CCGTCGGGTC ACATGACCTG
    CTAGCTCTCA TCCAGGTGTA TGCTGAGGGG GTAGAGCTTT TGGAACCACT ACATGAAGGA
    GGACAGGAAG GTGGAGAGAC TGCATTGCAC TATGCCGTGA GGACAGCTGA CCAAACCTCA
    CTGCACCTGG TCGACTTCCT TGTCCAGAAC AGTGGGAATT TGGATAAGCA GACGGAGCGA
    GGAAACACGG CCCTGCACTA TTGCTGCCTG TACGAGAAAC ATGAATGCCT CAAACTACTG
    CTCCGAGGCA AACCAGCTAC TGATATCACC AATCAGAATG GGGACACAGC TTTGGATGTG
    GCTAGGCGGA TGAAGCTCTT CCAGTGTGAG GAAGCATTGG TACAAGCTGC TGCTGGAAAA
    TTTAATACTA AAGTCCATGT GGAATATGAA TGGAATCTCA GGTTGGAGGA GCTTGATGAA
    AGTGATGATG ATCTGGATTA CAAACCAAGT CCCATTAAGA AGGATCGCTC ACCCCGTCCG
    CAGAGTTTCT GCCACTCATC CAGCCTCTCC CCTCATGATA AGCTCTCTCT GCCTGCTTTC
    ATTGGTCAGC GTGATAAGCA ACGCCTCTCC TACTCTGCAT TCACCAATCA GATGTACAGC
    GTTTCCACCG ACTGTCCGTC CTCTCCGGTT TCAGATGCTC CACCTCTGCC GCCAAGGAAT
    GCTGGCAAAG CTCCATCTCT GGCTTTCCTT GGCTCTCATT CCCACTATGC TACTCTGGCT
    AGCACTCGAC CATACATCAG AAGTGATTCA ACGCCGCCTC CTGTCAACAA GATGTCCCCT
    GTAACTAATA GATTTGAGGG CATCCCACAA CAGCAAAGTC CCAACACCAC CAAGCCCACA
    CTTGGTCCAA GGGTCCTTCC CAAACTACCT CAGAAGGTTG CCCTGCGTAA GATAGACACC
    ATACATCACC CATCTGTGGA CAAAACCAAC CAGCCTTCTG AAGCTGTCCT TTTTCAGAAA
    GTTCTGCAGA GTTCAGATAC ATCCCAGAAA GTTCCGATAG CTGACAGGCC TCAGCCTGGA
    GACGTCGCTC CACAGCGACC ACAGCCTTCA GAATTACCAC CTAAACCAGG AGAACTGCCT
    CCAAAGCCTC AACTGTCCGA ACTTCCCCCT AAACCCCAGT TGGGTGACCT ACCACCTAAA
    CCTCAGCTTA AAGATCTCCC GCCCAAGCCC CAACTCGCAG ACATCTCCCC AGCCAAACCC
    ATAACCATTG AGGTCCCCCC AAAACCTTCT CCAGGAGAAG TGGCTCCCAA GCCCCAACCT
    CCAGATACTC CAACAATCAT TCAACAAGGT GAACTGTCAC CTCAGCCTGT GATCCAGTCT
    AGTGAAGATA CCAATGGAAG TCCAACATCT GCACAGGACA CACCCGTACC ATTGCCCAGA
    AAAATAAATC CGATTAAGAG TAAAATGCGA AGGGTGAAGA CTATTTATGA TTGCCAGGCA
    GACAATGATG ATGAGCTGAC TTTCGCTGAG GGTGAGGTCA TTGTGGTAAC TGGTGAGGAA
    GATCAGGAGT GGTGGATTGG GCACATCGAA GGACAGCCGG AGAGAAAAGG TGTATTTCCC
    ATGTCCTTTG TTCATATCTT ATCAGATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005162662.2
    CDS169..3498
    Translation

    Target ORF information:

    RefSeq Version XM_005162605.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 1b (asap1b), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005162605.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGATGAGGT CATCATCCTC TCGTCTTAGT TTTTCCTCTA AGGAACCCAT ATGGAACAGG 
    ATGCCAGATC AAATATCGGT GTCGGAGTTT CTGTCGGAAA CAACAGAGGA TTACAACTCT
    CCCACTACAT CCAGCTTCAC CACCCGCTTG CAGAGCTGCA GGAATACAGT CAGCGTGTTA
    GAAGAGGCTT TGGATCAGGA CAGAACGTCT TTGCAGAAGG TCAAGAAGTC AGTCAAAGCC
    ATTTACAACT CTGGCCAAGA TCACATTCAA AATGAGGAAA CGTATTCCCA GGCATTGGAT
    AAATTTGGCA GCAACTTCAT CAGTCGGGAT AATCCAGATC TGGGCACAGC CTTTGTTAAA
    TTCTCCACTT TGACTAAAGA GCTTTCTGCC CTCCTCAAGA ACCTGCTTCA GAATTTAAGT
    CATAATGTGA TCTTTACACT GGACTCCCTG TTGAAAGGCG ACCTGAAAGG CGTGAAGGGG
    GACCTGAAAA AACCCTTTGA CAAGGCTTGG AAGGACTACG AAACCAAGTT TACAAAAATC
    GAAAAGGAAA AACGTGAGCA TGCCAAACAG CATGGAATGA TCCGAACAGA GATCACTGGT
    GCTGAGATCG CAGAGGAGAT GGAGAAAGAA AGGAGGCTCT TCCAGCTACA GATGTGTGAG
    TACCTGATCA AAGTAAATGA AATAAAGACC AAGAAAGGAG TTGACCTCCT TCAGAACTTG
    ATCAAGTATT ACCACGCGCA ATGCAACTTT TTCCAAGATG GCTTGAAGAC GGCAGATAAA
    CTGAAGCAGT ACATTGAGAA GTTAGCAGCT GATCTTTACA ATATAAAACA AACACAAGAT
    GAAGAGAAAA AGCAGCTCAC GGCTTTAAGA GATCTCATTA AATCCTCTCT ACAAGTGGAC
    CAAAAGGAGT CTAGAAGACA GGACTCTCAG AGCAAACAGG GTGGCTACAG TATGCACCAG
    CTGCAGGGTA ACAAAGAGTT TGGCTGTGAG AAGAAAGGCT ATCTGTTAAA GAAGAGTGAT
    GGGTTAAGGA AGGTGTGGCA GAGAAGGAAG TGCTCAGTTA AAGGCGGGAT TCTCACCATC
    TCTCACGCCA CTTCAAACAG GCAGCCTGTC AAACTCAACC TGCTCACATG CCAGGTCAAG
    CCCAGTGCTG AGGACAGAAA GTGCTTCGAT CTTATTTCCC ATAACCGCAC ATATCACTTT
    CAGGCAGAAG ATGAGCAGGA ATTTATAATA TGGATCTCAG TGTTAACTAA CAGCAAGGAG
    GAGGCACTTA ACATGGCCTT CAGAGGAGAG CAGGGTGGAG GAGATGATGG GCTGGAGGAT
    CTCACCAAAG CCATAATCGA TGATGTGCTG CGCATGCCGG GAAATGAGTG GTGCTGTGAT
    TGTGGGGCTC CAGATCCCAA ATGGTTGTCG ACTAACCTGG GAATCCTGAC ATGCATCGAA
    TGTTCAGGCA TACACCGGGA GATGGGCGTC CACATCTCAC GCATCCAGTC TATGGAGCTA
    GACAAACTGG GAACCTCCGA ACTCTTGCTG GCCAAGAATG TGGGGAACAG TAGTTTTAAT
    GATATAATGG AGGGAGGTCT TTCTTGTCCC TCTTCTAAAC CCACTCCCTC TAGTGACATG
    ACTGTTCGTA AGGAGTTCAT CAATGCTAAA TATGTAGATC ATAAGTTTGC AAGGAAAACC
    TGCAGCTCAG CAGAAGACAA GAGGAGTGAT CTTTTTAAAG CCGTCGGGTC ACATGACCTG
    CTAGCTCTCA TCCAGGTGTA TGCTGAGGGG GTAGAGCTTT TGGAACCACT ACATGAAGGA
    GGACAGGAAG GTGGAGAGAC TGCATTGCAC TATGCCGTGA GGACAGCTGA CCAAACCTCA
    CTGCACCTGG TCGACTTCCT TGTCCAGAAC AGTGGGAATT TGGATAAGCA GACGGAGCGA
    GGAAACACGG CCCTGCACTA TTGCTGCCTG TACGAGAAAC ATGAATGCCT CAAACTACTG
    CTCCGAGGCA AACCAGCTAC TGATATCACC AATCAGAATG GGGACACAGC TTTGGATGTG
    GCTAGGCGGA TGAAGCTCTT CCAGTGTGAG GAAGCATTGG TACAAGCTGC TGCTGGAAAA
    TTTAATACTA AAGTCCATGT GGAATATGAA TGGAATCTCA GGTTGGAGGA GCTTGATGAA
    AGTGATGATG ATCTGGATTA CAAACCAAGT CCCATTAAGA AGGATCGCTC ACCCCGTCCG
    CAGAGTTTCT GCCACTCATC CAGCCTCTCC CCTCATGATA AGCTCTCTCT GCCTGCTTTC
    ATTGGTCAGC GTGATAAGCA ACGCCTCTCC TACTCTGCAT TCACCAATCA GATGTACAGC
    GTTTCCACCG ACTGTCCGTC CTCTCCGGTT TCAGATGCTC CACCTCTGCC GCCAAGGAAT
    GCTGGCAAAG CTCCATCTCT GGCTTTCCTT GGCTCTCATT CCCACTATGC TACTCTGGCT
    AGCACTCGAC CATACATCAG AAGTGATTCA ACGCCGCCTC CTGTCAACAA GATGTCCCCT
    GTAACTAATA GATTTGAGGG CATCCCACAA CAGCAAAGTC CCAACACCAC CAAGCCCACA
    CTTGGTCCAA GGGTCCTTCC CAAACTACCT CAGAAGGTTG CCCTGCGTAA GATAGACACC
    ATACATCACC CATCTGTGGA CAAAACCAAC CAGCCTTCTG AAGCTGTCCT TTTTCAGAAA
    GTTCTGCAGA GTTCAGATAC ATCCCAGAAA GTTCCGATAG CTGACAGGCC TCAGCCTGGA
    GACGTCGCTC CACAGCGACC ACAGCCTTCA GAATTACCAC CTAAACCAGG AGAACTGCCT
    CCAAAGCCTC AACTGTCCGA ACTTCCCCCT AAACCCCAGT TGGGTGACCT ACCACCTAAA
    CCTCAGCTTA AAGATCTCCC GCCCAAGCCC CAACTCGCAG ACATCTCCCC AGCCAAACCC
    ATAACCATTG AGGTCCCCCC AAAACCTTCT CCAGGAGAAG TGGCTCCCAA GCCCCAACCT
    CCAGATACTC CAACAATCAT TCAACAAGGT GAACTGTCAC CTCAGCCTGT GATCCAGTCT
    AGTGAAGATA CCAATGGAAG TCCAACATCT GCACAGGACA CACCCGTACC ATTGCCCAGA
    AAAATAAATC CGATTAAGAG TAAAATGCGA AGGGTGAAGA CTATTTATGA TTGCCAGGCA
    GACAATGATG ATGAGCTGAC TTTCGCTGAG GGTGAGGTCA TTGTGGTAAC TGGTGAGGAA
    GATCAGGAGT GGTGGATTGG GCACATCGAA GGACAGCCGG AGAGAAAAGG TGTATTTCCC
    ATGTCCTTTG TTCATATCTT ATCAGATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa151790
    Clone ID Related Accession (Same CDS sequence) XM_005162606.4
    Accession Version XM_005162606.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3261bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005162606.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGATGAGGT CATCATCCTC TCGTCTTAGT TTTTCCTCTA AGGAACCCAT ATGGAACAGG 
    ATGCCAGATC AAATATCGGT GTCGGAGTTT CTGTCGGAAA CAACAGAGGA TTACAACTCT
    CCCACTACAT CCAGCTTCAC CACCCGCTTG CAGAGCTGCA GGAATACAGT CAGCGTGTTA
    GAAGAGGCTT TGGATCAGGA CAGAACGTCT TTGCAGAAGG TCAAGAAGTC AGTCAAAGCC
    ATTTACAACT CTGGCCAAGA TCACATTCAA AATGAGGAAA CGTATTCCCA GGCATTGGAT
    AAATTTGGCA GCAACTTCAT CAGTCGGGAT AATCCAGATC TGGGCACAGC CTTTGTTAAA
    TTCTCCACTT TGACTAAAGA GCTTTCTGCC CTCCTCAAGA ACCTGCTTCA GAATTTAAGT
    CATAATGTGA TCTTTACACT GGACTCCCTG TTGAAAGGCG ACCTGAAAGG CGTGAAGGGG
    GACCTGAAAA AACCCTTTGA CAAGGCTTGG AAGGACTACG AAACCAAGTT TACAAAAATC
    GAAAAGGAAA AACGTGAGCA TGCCAAACAG CATGGAATGA TCCGAACAGA GATCACTGGT
    GCTGAGATCG CAGAGGAGAT GGAGAAAGAA AGGAGGCTCT TCCAGCTACA GATGTGTGAG
    TACCTGATCA AAGTAAATGA AATAAAGACC AAGAAAGGAG TTGACCTCCT TCAGAACTTG
    ATCAAGTATT ACCACGCGCA ATGCAACTTT TTCCAAGATG GCTTGAAGAC GGCAGATAAA
    CTGAAGCAGT ACATTGAGAA GTTAGCAGCT GATCTTTACA ATATAAAACA AACACAAGAT
    GAAGAGAAAA AGCAGCTCAC GGCTTTAAGA GATCTCATTA AATCCTCTCT ACAAGTGGAC
    CAAAAGGAGT CTAGAAGACA GGACTCTCAG AGCAAACAGG GTGGCTACAG TATGCACCAG
    CTGCAGGGTA ACAAAGAGTT TGGCTGTGAG AAGAAAGGCT ATCTGTTAAA GAAGAGTGAT
    GGGTTAAGGA AGGTGTGGCA GAGAAGGAAG TGCTCAGTTA AAGGCGGGAT TCTCACCATC
    TCTCACGCCA CTTCAAACAG GCAGCCTGTC AAACTCAACC TGCTCACATG CCAGGTCAAG
    CCCAGTGCTG AGGACAGAAA GTGCTTCGAT CTTATTTCCC ATAACCGCAC ATATCACTTT
    CAGGCAGAAG ATGAGCAGGA ATTTATAATA TGGATCTCAG TGTTAACTAA CAGCAAGGAG
    GAGGCACTTA ACATGGCCTT CAGAGGAGAG CAGGGTGGAG GAGATGATGG GCTGGAGGAT
    CTCACCAAAG CCATAATCGA TGATGTGCTG CGCATGCCGG GAAATGAGTG GTGCTGTGAT
    TGTGGGGCTC CAGATCCCAA ATGGTTGTCG ACTAACCTGG GAATCCTGAC ATGCATCGAA
    TGTTCAGGCA TACACCGGGA GATGGGCGTC CACATCTCAC GCATCCAGTC TATGGAGCTA
    GACAAACTGG GAACCTCCGA ACTCTTGCTG GCCAAGAATG TGGGGAACAG TAGTTTTAAT
    GATATAATGG AGGGAGGTCT TTCTTGTCCC TCTTCTAAAC CCACTCCCTC TAGTGACATG
    ACTGTTCGTA AGGAGTTCAT CAATGCTAAA TATGTAGATC ATAAGTTTGC AAGGAAAACC
    TGCAGCTCAG CAGAAGACAA GAGGAGTGAT CTTTTTAAAG CCGTCGGGTC ACATGACCTG
    CTAGCTCTCA TCCAGGTGTA TGCTGAGGGG GTAGAGCTTT TGGAACCACT ACATGAAGGA
    GGACAGGAAG GTGGAGAGAC TGCATTGCAC TATGCCGTGA GGACAGCTGA CCAAACCTCA
    CTGCACCTGG TCGACTTCCT TGTCCAGAAC AGTGGGAATT TGGATAAGCA GACGGAGCGA
    GGAAACACGG CCCTGCACTA TTGCTGCCTG TACGAGAAAC ATGAATGCCT CAAACTACTG
    CTCCGAGGCA AACCAGCTAC TGATATCACC AATCAGAATG GGGACACAGC TTTGGATGTG
    GCTAGGCGGA TGAAGCTCTT CCAGTGTGAG GAAGCATTGG TACAAGCTGC TGCTGGAAAA
    TTTAATACTA AAGTCCATGT GGAATATGAA TGGAATCTCA GGTTGGAGGA GCTTGATGAA
    AGTGATGATG ATCTGGATTA CAAACCAAGT CCCATTAAGA AGGATCGCTC ACCCCGTCCG
    CAGAGTTTCT GCCACTCATC CAGCCTCTCC CCTCATGATA AGCTCTCTCT GCCTGCTTTC
    ATTGGTCAGC GTGATAAGCA ACGCCTCTCC TACTCTGCAT TCACCAATCA GATGTACAGC
    GTTTCCACCG ACTGTCCGTC CTCTCCGGTT TCAGATGCTC CACCTCTGCC GCCAAGGAAT
    GCTGGCAAAG GAAGTGATTC AACGCCGCCT CCTGTCAACA AGATGTCCCC TGTAACTAAT
    AGATTTGAGG GCATCCCACA ACAGCAAAGT CCCAACACCA CCAAGCCCAC ACTTGGTCCA
    AGGGTCCTTC CCAAACTACC TCAGAAGGTT GCCCTGCGTA AGATAGACAC CATACATCAC
    CCATCTGTGG ACAAAACCAA CCAGCCTTCT GAAGCTGTCC TTTTTCAGAA AGTTCTGCAG
    AGTTCAGATA CATCCCAGAA AGTTCCGATA GCTGACAGGC CTCAGCCTGG AGACGTCGCT
    CCACAGCGAC CACAGCCTTC AGAATTACCA CCTAAACCAG GAGAACTGCC TCCAAAGCCT
    CAACTGTCCG AACTTCCCCC TAAACCCCAG TTGGGTGACC TACCACCTAA ACCTCAGCTT
    AAAGATCTCC CGCCCAAGCC CCAACTCGCA GACATCTCCC CAGCCAAACC CATAACCATT
    GAGGTCCCCC CAAAACCTTC TCCAGGAGAA GTGGCTCCCA AGCCCCAACC TCCAGATACT
    CCAACAATCA TTCAACAAGG TGAACTGTCA CCTCAGCCTG TGATCCAGTC TAGTGAAGAT
    ACCAATGGAA GTCCAACATC TGCACAGGAC ACACCCGTAC CATTGCCCAG AAAAATAAAT
    CCGATTAAGA GTAAAATGCG AAGGGTGAAG ACTATTTATG ATTGCCAGGC AGACAATGAT
    GATGAGCTGA CTTTCGCTGA GGGTGAGGTC ATTGTGGTAA CTGGTGAGGA AGATCAGGAG
    TGGTGGATTG GGCACATCGA AGGACAGCCG GAGAGAAAAG GTGTATTTCC CATGTCCTTT
    GTTCATATCT TATCAGATTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005162663.2
    CDS169..3429
    Translation

    Target ORF information:

    RefSeq Version XM_005162606.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 1b (asap1b), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005162606.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGATGAGGT CATCATCCTC TCGTCTTAGT TTTTCCTCTA AGGAACCCAT ATGGAACAGG 
    ATGCCAGATC AAATATCGGT GTCGGAGTTT CTGTCGGAAA CAACAGAGGA TTACAACTCT
    CCCACTACAT CCAGCTTCAC CACCCGCTTG CAGAGCTGCA GGAATACAGT CAGCGTGTTA
    GAAGAGGCTT TGGATCAGGA CAGAACGTCT TTGCAGAAGG TCAAGAAGTC AGTCAAAGCC
    ATTTACAACT CTGGCCAAGA TCACATTCAA AATGAGGAAA CGTATTCCCA GGCATTGGAT
    AAATTTGGCA GCAACTTCAT CAGTCGGGAT AATCCAGATC TGGGCACAGC CTTTGTTAAA
    TTCTCCACTT TGACTAAAGA GCTTTCTGCC CTCCTCAAGA ACCTGCTTCA GAATTTAAGT
    CATAATGTGA TCTTTACACT GGACTCCCTG TTGAAAGGCG ACCTGAAAGG CGTGAAGGGG
    GACCTGAAAA AACCCTTTGA CAAGGCTTGG AAGGACTACG AAACCAAGTT TACAAAAATC
    GAAAAGGAAA AACGTGAGCA TGCCAAACAG CATGGAATGA TCCGAACAGA GATCACTGGT
    GCTGAGATCG CAGAGGAGAT GGAGAAAGAA AGGAGGCTCT TCCAGCTACA GATGTGTGAG
    TACCTGATCA AAGTAAATGA AATAAAGACC AAGAAAGGAG TTGACCTCCT TCAGAACTTG
    ATCAAGTATT ACCACGCGCA ATGCAACTTT TTCCAAGATG GCTTGAAGAC GGCAGATAAA
    CTGAAGCAGT ACATTGAGAA GTTAGCAGCT GATCTTTACA ATATAAAACA AACACAAGAT
    GAAGAGAAAA AGCAGCTCAC GGCTTTAAGA GATCTCATTA AATCCTCTCT ACAAGTGGAC
    CAAAAGGAGT CTAGAAGACA GGACTCTCAG AGCAAACAGG GTGGCTACAG TATGCACCAG
    CTGCAGGGTA ACAAAGAGTT TGGCTGTGAG AAGAAAGGCT ATCTGTTAAA GAAGAGTGAT
    GGGTTAAGGA AGGTGTGGCA GAGAAGGAAG TGCTCAGTTA AAGGCGGGAT TCTCACCATC
    TCTCACGCCA CTTCAAACAG GCAGCCTGTC AAACTCAACC TGCTCACATG CCAGGTCAAG
    CCCAGTGCTG AGGACAGAAA GTGCTTCGAT CTTATTTCCC ATAACCGCAC ATATCACTTT
    CAGGCAGAAG ATGAGCAGGA ATTTATAATA TGGATCTCAG TGTTAACTAA CAGCAAGGAG
    GAGGCACTTA ACATGGCCTT CAGAGGAGAG CAGGGTGGAG GAGATGATGG GCTGGAGGAT
    CTCACCAAAG CCATAATCGA TGATGTGCTG CGCATGCCGG GAAATGAGTG GTGCTGTGAT
    TGTGGGGCTC CAGATCCCAA ATGGTTGTCG ACTAACCTGG GAATCCTGAC ATGCATCGAA
    TGTTCAGGCA TACACCGGGA GATGGGCGTC CACATCTCAC GCATCCAGTC TATGGAGCTA
    GACAAACTGG GAACCTCCGA ACTCTTGCTG GCCAAGAATG TGGGGAACAG TAGTTTTAAT
    GATATAATGG AGGGAGGTCT TTCTTGTCCC TCTTCTAAAC CCACTCCCTC TAGTGACATG
    ACTGTTCGTA AGGAGTTCAT CAATGCTAAA TATGTAGATC ATAAGTTTGC AAGGAAAACC
    TGCAGCTCAG CAGAAGACAA GAGGAGTGAT CTTTTTAAAG CCGTCGGGTC ACATGACCTG
    CTAGCTCTCA TCCAGGTGTA TGCTGAGGGG GTAGAGCTTT TGGAACCACT ACATGAAGGA
    GGACAGGAAG GTGGAGAGAC TGCATTGCAC TATGCCGTGA GGACAGCTGA CCAAACCTCA
    CTGCACCTGG TCGACTTCCT TGTCCAGAAC AGTGGGAATT TGGATAAGCA GACGGAGCGA
    GGAAACACGG CCCTGCACTA TTGCTGCCTG TACGAGAAAC ATGAATGCCT CAAACTACTG
    CTCCGAGGCA AACCAGCTAC TGATATCACC AATCAGAATG GGGACACAGC TTTGGATGTG
    GCTAGGCGGA TGAAGCTCTT CCAGTGTGAG GAAGCATTGG TACAAGCTGC TGCTGGAAAA
    TTTAATACTA AAGTCCATGT GGAATATGAA TGGAATCTCA GGTTGGAGGA GCTTGATGAA
    AGTGATGATG ATCTGGATTA CAAACCAAGT CCCATTAAGA AGGATCGCTC ACCCCGTCCG
    CAGAGTTTCT GCCACTCATC CAGCCTCTCC CCTCATGATA AGCTCTCTCT GCCTGCTTTC
    ATTGGTCAGC GTGATAAGCA ACGCCTCTCC TACTCTGCAT TCACCAATCA GATGTACAGC
    GTTTCCACCG ACTGTCCGTC CTCTCCGGTT TCAGATGCTC CACCTCTGCC GCCAAGGAAT
    GCTGGCAAAG GAAGTGATTC AACGCCGCCT CCTGTCAACA AGATGTCCCC TGTAACTAAT
    AGATTTGAGG GCATCCCACA ACAGCAAAGT CCCAACACCA CCAAGCCCAC ACTTGGTCCA
    AGGGTCCTTC CCAAACTACC TCAGAAGGTT GCCCTGCGTA AGATAGACAC CATACATCAC
    CCATCTGTGG ACAAAACCAA CCAGCCTTCT GAAGCTGTCC TTTTTCAGAA AGTTCTGCAG
    AGTTCAGATA CATCCCAGAA AGTTCCGATA GCTGACAGGC CTCAGCCTGG AGACGTCGCT
    CCACAGCGAC CACAGCCTTC AGAATTACCA CCTAAACCAG GAGAACTGCC TCCAAAGCCT
    CAACTGTCCG AACTTCCCCC TAAACCCCAG TTGGGTGACC TACCACCTAA ACCTCAGCTT
    AAAGATCTCC CGCCCAAGCC CCAACTCGCA GACATCTCCC CAGCCAAACC CATAACCATT
    GAGGTCCCCC CAAAACCTTC TCCAGGAGAA GTGGCTCCCA AGCCCCAACC TCCAGATACT
    CCAACAATCA TTCAACAAGG TGAACTGTCA CCTCAGCCTG TGATCCAGTC TAGTGAAGAT
    ACCAATGGAA GTCCAACATC TGCACAGGAC ACACCCGTAC CATTGCCCAG AAAAATAAAT
    CCGATTAAGA GTAAAATGCG AAGGGTGAAG ACTATTTATG ATTGCCAGGC AGACAATGAT
    GATGAGCTGA CTTTCGCTGA GGGTGAGGTC ATTGTGGTAA CTGGTGAGGA AGATCAGGAG
    TGGTGGATTG GGCACATCGA AGGACAGCCG GAGAGAAAAG GTGTATTTCC CATGTCCTTT
    GTTCATATCT TATCAGATTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa151791
    Clone ID Related Accession (Same CDS sequence) XM_003201277.5
    Accession Version XM_003201277.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3249bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_003201277.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGATGAGGT CATCATCCTC TCGTCTTAGT TTTTCCTCTA AGGAACCCAT ATGGAACAGG 
    ATGCCAGATC AAATATCGGT GTCGGAGTTT CTGTCGGAAA CAACAGAGGA TTACAACTCT
    CCCACTACAT CCAGCTTCAC CACCCGCTTG CAGAGCTGCA GGAATACAGT CAGCGTGTTA
    GAAGAGGCTT TGGATCAGGA CAGAACGTCT TTGCAGAAGG TCAAGAAGTC AGTCAAAGCC
    ATTTACAACT CTGGCCAAGA TCACATTCAA AATGAGGAAA CGTATTCCCA GGCATTGGAT
    AAATTTGGCA GCAACTTCAT CAGTCGGGAT AATCCAGATC TGGGCACAGC CTTTGTTAAA
    TTCTCCACTT TGACTAAAGA GCTTTCTGCC CTCCTCAAGA ACCTGCTTCA GAATTTAAGT
    CATAATGTGA TCTTTACACT GGACTCCCTG TTGAAAGGCG ACCTGAAAGG CGTGAAGGGG
    GACCTGAAAA AACCCTTTGA CAAGGCTTGG AAGGACTACG AAACCAAGTT TACAAAAATC
    GAAAAGGAAA AACGTGAGCA TGCCAAACAG CATGGAATGA TCCGAACAGA GATCACTGGT
    GCTGAGATCG CAGAGGAGAT GGAGAAAGAA AGGAGGCTCT TCCAGCTACA GATGTGTGAG
    TACCTGATCA AAGTAAATGA AATAAAGACC AAGAAAGGAG TTGACCTCCT TCAGAACTTG
    ATCAAGTATT ACCACGCGCA ATGCAACTTT TTCCAAGATG GCTTGAAGAC GGCAGATAAA
    CTGAAGCAGT ACATTGAGAA GTTAGCAGCT GATCTTTACA ATATAAAACA AACACAAGAT
    GAAGAGAAAA AGCAGCTCAC GGCTTTAAGA GATCTCATTA AATCCTCTCT ACAAGTGGAC
    CAAAAGGAGG ACTCTCAGAG CAAACAGGGT GGCTACAGTA TGCACCAGCT GCAGGGTAAC
    AAAGAGTTTG GCTGTGAGAA GAAAGGCTAT CTGTTAAAGA AGAGTGATGG GTTAAGGAAG
    GTGTGGCAGA GAAGGAAGTG CTCAGTTAAA GGCGGGATTC TCACCATCTC TCACGCCACT
    TCAAACAGGC AGCCTGTCAA ACTCAACCTG CTCACATGCC AGGTCAAGCC CAGTGCTGAG
    GACAGAAAGT GCTTCGATCT TATTTCCCAT AACCGCACAT ATCACTTTCA GGCAGAAGAT
    GAGCAGGAAT TTATAATATG GATCTCAGTG TTAACTAACA GCAAGGAGGA GGCACTTAAC
    ATGGCCTTCA GAGGAGAGCA GGGTGGAGGA GATGATGGGC TGGAGGATCT CACCAAAGCC
    ATAATCGATG ATGTGCTGCG CATGCCGGGA AATGAGTGGT GCTGTGATTG TGGGGCTCCA
    GATCCCAAAT GGTTGTCGAC TAACCTGGGA ATCCTGACAT GCATCGAATG TTCAGGCATA
    CACCGGGAGA TGGGCGTCCA CATCTCACGC ATCCAGTCTA TGGAGCTAGA CAAACTGGGA
    ACCTCCGAAC TCTTGCTGGC CAAGAATGTG GGGAACAGTA GTTTTAATGA TATAATGGAG
    GGAGGTCTTT CTTGTCCCTC TTCTAAACCC ACTCCCTCTA GTGACATGAC TGTTCGTAAG
    GAGTTCATCA ATGCTAAATA TGTAGATCAT AAGTTTGCAA GGAAAACCTG CAGCTCAGCA
    GAAGACAAGA GGAGTGATCT TTTTAAAGCC GTCGGGTCAC ATGACCTGCT AGCTCTCATC
    CAGGTGTATG CTGAGGGGGT AGAGCTTTTG GAACCACTAC ATGAAGGAGG ACAGGAAGGT
    GGAGAGACTG CATTGCACTA TGCCGTGAGG ACAGCTGACC AAACCTCACT GCACCTGGTC
    GACTTCCTTG TCCAGAACAG TGGGAATTTG GATAAGCAGA CGGAGCGAGG AAACACGGCC
    CTGCACTATT GCTGCCTGTA CGAGAAACAT GAATGCCTCA AACTACTGCT CCGAGGCAAA
    CCAGCTACTG ATATCACCAA TCAGAATGGG GACACAGCTT TGGATGTGGC TAGGCGGATG
    AAGCTCTTCC AGTGTGAGGA AGCATTGGTA CAAGCTGCTG CTGGAAAATT TAATACTAAA
    GTCCATGTGG AATATGAATG GAATCTCAGG TTGGAGGAGC TTGATGAAAG TGATGATGAT
    CTGGATTACA AACCAAGTCC CATTAAGAAG GATCGCTCAC CCCGTCCGCA GAGTTTCTGC
    CACTCATCCA GCCTCTCCCC TCATGATAAG CTCTCTCTGC CTGCTTTCAT TGGTCAGCGT
    GATAAGCAAC GCCTCTCCTA CTCTGCATTC ACCAATCAGA TGTACAGCGT TTCCACCGAC
    TGTCCGTCCT CTCCGGTTTC AGATGCTCCA CCTCTGCCGC CAAGGAATGC TGGCAAAGGA
    AGTGATTCAA CGCCGCCTCC TGTCAACAAG ATGTCCCCTG TAACTAATAG ATTTGAGGGC
    ATCCCACAAC AGCAAAGTCC CAACACCACC AAGCCCACAC TTGGTCCAAG GGTCCTTCCC
    AAACTACCTC AGAAGGTTGC CCTGCGTAAG ATAGACACCA TACATCACCC ATCTGTGGAC
    AAAACCAACC AGCCTTCTGA AGCTGTCCTT TTTCAGAAAG TTCTGCAGAG TTCAGATACA
    TCCCAGAAAG TTCCGATAGC TGACAGGCCT CAGCCTGGAG ACGTCGCTCC ACAGCGACCA
    CAGCCTTCAG AATTACCACC TAAACCAGGA GAACTGCCTC CAAAGCCTCA ACTGTCCGAA
    CTTCCCCCTA AACCCCAGTT GGGTGACCTA CCACCTAAAC CTCAGCTTAA AGATCTCCCG
    CCCAAGCCCC AACTCGCAGA CATCTCCCCA GCCAAACCCA TAACCATTGA GGTCCCCCCA
    AAACCTTCTC CAGGAGAAGT GGCTCCCAAG CCCCAACCTC CAGATACTCC AACAATCATT
    CAACAAGGTG AACTGTCACC TCAGCCTGTG ATCCAGTCTA GTGAAGATAC CAATGGAAGT
    CCAACATCTG CACAGGACAC ACCCGTACCA TTGCCCAGAA AAATAAATCC GATTAAGAGT
    AAAATGCGAA GGGTGAAGAC TATTTATGAT TGCCAGGCAG ACAATGATGA TGAGCTGACT
    TTCGCTGAGG GTGAGGTCAT TGTGGTAACT GGTGAGGAAG ATCAGGAGTG GTGGATTGGG
    CACATCGAAG GACAGCCGGA GAGAAAAGGT GTATTTCCCA TGTCCTTTGT TCATATCTTA
    TCAGATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003201325.2
    CDS169..3417
    Translation

    Target ORF information:

    RefSeq Version XM_003201277.5
    Organism Danio rerio(zebrafish)
    Definition Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 1b (asap1b), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003201277.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGATGAGGT CATCATCCTC TCGTCTTAGT TTTTCCTCTA AGGAACCCAT ATGGAACAGG 
    ATGCCAGATC AAATATCGGT GTCGGAGTTT CTGTCGGAAA CAACAGAGGA TTACAACTCT
    CCCACTACAT CCAGCTTCAC CACCCGCTTG CAGAGCTGCA GGAATACAGT CAGCGTGTTA
    GAAGAGGCTT TGGATCAGGA CAGAACGTCT TTGCAGAAGG TCAAGAAGTC AGTCAAAGCC
    ATTTACAACT CTGGCCAAGA TCACATTCAA AATGAGGAAA CGTATTCCCA GGCATTGGAT
    AAATTTGGCA GCAACTTCAT CAGTCGGGAT AATCCAGATC TGGGCACAGC CTTTGTTAAA
    TTCTCCACTT TGACTAAAGA GCTTTCTGCC CTCCTCAAGA ACCTGCTTCA GAATTTAAGT
    CATAATGTGA TCTTTACACT GGACTCCCTG TTGAAAGGCG ACCTGAAAGG CGTGAAGGGG
    GACCTGAAAA AACCCTTTGA CAAGGCTTGG AAGGACTACG AAACCAAGTT TACAAAAATC
    GAAAAGGAAA AACGTGAGCA TGCCAAACAG CATGGAATGA TCCGAACAGA GATCACTGGT
    GCTGAGATCG CAGAGGAGAT GGAGAAAGAA AGGAGGCTCT TCCAGCTACA GATGTGTGAG
    TACCTGATCA AAGTAAATGA AATAAAGACC AAGAAAGGAG TTGACCTCCT TCAGAACTTG
    ATCAAGTATT ACCACGCGCA ATGCAACTTT TTCCAAGATG GCTTGAAGAC GGCAGATAAA
    CTGAAGCAGT ACATTGAGAA GTTAGCAGCT GATCTTTACA ATATAAAACA AACACAAGAT
    GAAGAGAAAA AGCAGCTCAC GGCTTTAAGA GATCTCATTA AATCCTCTCT ACAAGTGGAC
    CAAAAGGAGG ACTCTCAGAG CAAACAGGGT GGCTACAGTA TGCACCAGCT GCAGGGTAAC
    AAAGAGTTTG GCTGTGAGAA GAAAGGCTAT CTGTTAAAGA AGAGTGATGG GTTAAGGAAG
    GTGTGGCAGA GAAGGAAGTG CTCAGTTAAA GGCGGGATTC TCACCATCTC TCACGCCACT
    TCAAACAGGC AGCCTGTCAA ACTCAACCTG CTCACATGCC AGGTCAAGCC CAGTGCTGAG
    GACAGAAAGT GCTTCGATCT TATTTCCCAT AACCGCACAT ATCACTTTCA GGCAGAAGAT
    GAGCAGGAAT TTATAATATG GATCTCAGTG TTAACTAACA GCAAGGAGGA GGCACTTAAC
    ATGGCCTTCA GAGGAGAGCA GGGTGGAGGA GATGATGGGC TGGAGGATCT CACCAAAGCC
    ATAATCGATG ATGTGCTGCG CATGCCGGGA AATGAGTGGT GCTGTGATTG TGGGGCTCCA
    GATCCCAAAT GGTTGTCGAC TAACCTGGGA ATCCTGACAT GCATCGAATG TTCAGGCATA
    CACCGGGAGA TGGGCGTCCA CATCTCACGC ATCCAGTCTA TGGAGCTAGA CAAACTGGGA
    ACCTCCGAAC TCTTGCTGGC CAAGAATGTG GGGAACAGTA GTTTTAATGA TATAATGGAG
    GGAGGTCTTT CTTGTCCCTC TTCTAAACCC ACTCCCTCTA GTGACATGAC TGTTCGTAAG
    GAGTTCATCA ATGCTAAATA TGTAGATCAT AAGTTTGCAA GGAAAACCTG CAGCTCAGCA
    GAAGACAAGA GGAGTGATCT TTTTAAAGCC GTCGGGTCAC ATGACCTGCT AGCTCTCATC
    CAGGTGTATG CTGAGGGGGT AGAGCTTTTG GAACCACTAC ATGAAGGAGG ACAGGAAGGT
    GGAGAGACTG CATTGCACTA TGCCGTGAGG ACAGCTGACC AAACCTCACT GCACCTGGTC
    GACTTCCTTG TCCAGAACAG TGGGAATTTG GATAAGCAGA CGGAGCGAGG AAACACGGCC
    CTGCACTATT GCTGCCTGTA CGAGAAACAT GAATGCCTCA AACTACTGCT CCGAGGCAAA
    CCAGCTACTG ATATCACCAA TCAGAATGGG GACACAGCTT TGGATGTGGC TAGGCGGATG
    AAGCTCTTCC AGTGTGAGGA AGCATTGGTA CAAGCTGCTG CTGGAAAATT TAATACTAAA
    GTCCATGTGG AATATGAATG GAATCTCAGG TTGGAGGAGC TTGATGAAAG TGATGATGAT
    CTGGATTACA AACCAAGTCC CATTAAGAAG GATCGCTCAC CCCGTCCGCA GAGTTTCTGC
    CACTCATCCA GCCTCTCCCC TCATGATAAG CTCTCTCTGC CTGCTTTCAT TGGTCAGCGT
    GATAAGCAAC GCCTCTCCTA CTCTGCATTC ACCAATCAGA TGTACAGCGT TTCCACCGAC
    TGTCCGTCCT CTCCGGTTTC AGATGCTCCA CCTCTGCCGC CAAGGAATGC TGGCAAAGGA
    AGTGATTCAA CGCCGCCTCC TGTCAACAAG ATGTCCCCTG TAACTAATAG ATTTGAGGGC
    ATCCCACAAC AGCAAAGTCC CAACACCACC AAGCCCACAC TTGGTCCAAG GGTCCTTCCC
    AAACTACCTC AGAAGGTTGC CCTGCGTAAG ATAGACACCA TACATCACCC ATCTGTGGAC
    AAAACCAACC AGCCTTCTGA AGCTGTCCTT TTTCAGAAAG TTCTGCAGAG TTCAGATACA
    TCCCAGAAAG TTCCGATAGC TGACAGGCCT CAGCCTGGAG ACGTCGCTCC ACAGCGACCA
    CAGCCTTCAG AATTACCACC TAAACCAGGA GAACTGCCTC CAAAGCCTCA ACTGTCCGAA
    CTTCCCCCTA AACCCCAGTT GGGTGACCTA CCACCTAAAC CTCAGCTTAA AGATCTCCCG
    CCCAAGCCCC AACTCGCAGA CATCTCCCCA GCCAAACCCA TAACCATTGA GGTCCCCCCA
    AAACCTTCTC CAGGAGAAGT GGCTCCCAAG CCCCAACCTC CAGATACTCC AACAATCATT
    CAACAAGGTG AACTGTCACC TCAGCCTGTG ATCCAGTCTA GTGAAGATAC CAATGGAAGT
    CCAACATCTG CACAGGACAC ACCCGTACCA TTGCCCAGAA AAATAAATCC GATTAAGAGT
    AAAATGCGAA GGGTGAAGAC TATTTATGAT TGCCAGGCAG ACAATGATGA TGAGCTGACT
    TTCGCTGAGG GTGAGGTCAT TGTGGTAACT GGTGAGGAAG ATCAGGAGTG GTGGATTGGG
    CACATCGAAG GACAGCCGGA GAGAAAAGGT GTATTTCCCA TGTCCTTTGT TCATATCTTA
    TCAGATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44735
    Clone ID Related Accession (Same CDS sequence) XM_009297312.3
    Accession Version XM_009297312.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3402bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009297312.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGATGAGGT CATCATCCTC TCGTCTTAGT TTTTCCTCTA AGGAACCCAT ATGGAACAGG 
    ATGCCAGATC AAATATCGGT GTCGGAGTTT CTGTCGGAAA CAACAGAGGA TTACAACTCT
    CCCACTACAT CCAGCTTCAC CACCCGCTTG CAGAGCTGCA GGAATACAGT CAGCGTGTTA
    GAAGAGGCTT TGGATCAGGA CAGAACGTCT TTGCAGAAGG TCAAGAAGTC AGTCAAAGCC
    ATTTACAACT CTGGCCAAGA TCACATTCAA AATGAGGAAA CGTATTCCCA GGCATTGGAT
    AAATTTGGCA GCAACTTCAT CAGTCGGGAT AATCCAGATC TGGGCACAGC CTTTGTTAAA
    TTCTCCACTT TGACTAAAGA GCTTTCTGCC CTCCTCAAGA ACCTGCTTCA GAATTTAAGT
    CATAATGTGA TCTTTACACT GGACTCCCTG TTGAAAGGCG ACCTGAAAGG CGTGAAGGGG
    GACCTGAAAA AACCCTTTGA CAAGGCTTGG AAGGACTACG AAACCAAGTT TACAAAAATC
    GAAAAGGAAA AACGTGAGCA TGCCAAACAG CATGGAATGA TCCGAACAGA GATCACTGGT
    GCTGAGATCG CAGAGGAGAT GGAGAAAGAA AGGAGGCTCT TCCAGCTACA GATGTGTGAG
    TACCTGATCA AAGTAAATGA AATAAAGACC AAGAAAGGAG TTGACCTCCT TCAGAACTTG
    ATCAAGTATT ACCACGCGCA ATGCAACTTT TTCCAAGATG GCTTGAAGAC GGCAGATAAA
    CTGAAGCAGT ACATTGAGAA GTTAGCAGCT GATCTTTACA ATATAAAACA AACACAAGAT
    GAAGAGAAAA AGCAGCTCAC GGCTTTAAGA GATCTCATTA AATCCTCTCT ACAAGTGGAC
    CAAAAGGAGG ACTCTCAGAG CAAACAGGGT GGCTACAGTA TGCACCAGCT GCAGGGTAAC
    AAAGAGTTTG GCTGTGAGAA GAAAGGCTAT CTGTTAAAGA AGAGTGATGG GTTAAGGAAG
    GTGTGGCAGA GAAGGAAGTG CTCAGTTAAA GGCGGGATTC TCACCATCTC TCACGCCACT
    TCAAACAGGC AGCCTGTCAA ACTCAACCTG CTCACATGCC AGGTCAAGCC CAGTGCTGAG
    GACAGAAAGT GCTTCGATCT TATTTCCCAT AACCGCACAT ATCACTTTCA GGCAGAAGAT
    GAGCAGGAAT TTATAATATG GATCTCAGTG TTAACTAACA GCAAGGAGGA GGCACTTAAC
    ATGGCCTTCA GAGGAGAGCA GGGTGGAGGA GATGATGGGC TGGAGGATCT CACCAAAGCC
    ATAATCGATG ATGTGCTGCG CATGCCGGGA AATGAGTGGT GCTGTGATTG TGGGGCTCCA
    GATCCCAAAT GGTTGTCGAC TAACCTGGGA ATCCTGACAT GCATCGAATG TTCAGGCATA
    CACCGGGAGA TGGGCGTCCA CATCTCACGC ATCCAGTCTA TGGAGCTAGA CAAACTGGGA
    ACCTCCGAAC TCTTGCTGGC CAAGAATGTG GGGAACAGTA GTTTTAATGA TATAATGGAG
    GGAGGTCTTT CTTGTCCCTC TTCTAAACCC ACTCCCTCTA GTGACATGAC TGTTCGTAAG
    GAGTTCATCA ATGCTAAATA TGTAGATCAT AAGTTTGCAA GGAAAACCTG CAGCTCAGCA
    GAAGACAAGA GGAGTGATCT TTTTAAAGCC GTCGGGTCAC ATGACCTGCT AGCTCTCATC
    CAGGTGTATG CTGAGGGGGT AGAGCTTTTG GAACCACTAC ATGAAGGAGG ACAGGAAGGT
    GGAGAGACTG CATTGCACTA TGCCGTGAGG ACAGCTGACC AAACCTCACT GCACCTGGTC
    GACTTCCTTG TCCAGAACAG TGGGAATTTG GATAAGCAGA CGGAGCGAGG AAACACGGCC
    CTGCACTATT GCTGCCTGTA CGAGAAACAT GAATGCCTCA AACTACTGCT CCGAGGCAAA
    CCAGCTACTG ATATCACCAA TCAGAATGGG GACACAGCTT TGGATGTGGC TAGGCGGATG
    AAGCTCTTCC AGTGTGAGGA AGCATTGGTA CAAGCTGCTG CTGGAAAATT TAATACTAAA
    GTCCATGTGG AATATGAATG GAATCTCAGG TTGGAGGAGC TTGATGAAAG TGATGATGAT
    CTGGATTACA AACCAAGTCC CATTAAGAAG GATCGCTCAC CCCGTCCGCA GAGTTTCTGC
    CACTCATCCA GCCTCTCCCC TCATGATAAG CTCTCTCTGC CTGCTTTCAT TGGTCAGCGT
    GATAAGCAAC GCCTCTCCTA CTCTGCATTC ACCAATCAGA TGTACAGCGT TTCCACCGAC
    TGTCCGTCCT CTCCGGTTTC AGATGCTCCA CCTCTGCCGC CAAGGAATGC TGGCAAAGCC
    CCTCCATTGT CGAACCTGCC TCCCTCCACC CTTGCCCCTG CATCACAGAC CTCACCTAGT
    GGCAGCTCTA CCCTGTCAAA GAAGAGACCC CCTCCCCCTC CCCCTGGACA CAAACGCACG
    CTATCTGACC CCCCTACCCC TCTCTTTAAA GGAAGTGATT CAACGCCGCC TCCTGTCAAC
    AAGATGTCCC CTGTAACTAA TAGATTTGAG GGCATCCCAC AACAGCAAAG TCCCAACACC
    ACCAAGCCCA CACTTGGTCC AAGGGTCCTT CCCAAACTAC CTCAGAAGGT TGCCCTGCGT
    AAGATAGACA CCATACATCA CCCATCTGTG GACAAAACCA ACCAGCCTTC TGAAGCTGTC
    CTTTTTCAGA AAGTTCTGCA GAGTTCAGAT ACATCCCAGA AAGTTCCGAT AGCTGACAGG
    CCTCAGCCTG GAGACGTCGC TCCACAGCGA CCACAGCCTT CAGAATTACC ACCTAAACCA
    GGAGAACTGC CTCCAAAGCC TCAACTGTCC GAACTTCCCC CTAAACCCCA GTTGGGTGAC
    CTACCACCTA AACCTCAGCT TAAAGATCTC CCGCCCAAGC CCCAACTCGC AGACATCTCC
    CCAGCCAAAC CCATAACCAT TGAGGTCCCC CCAAAACCTT CTCCAGGAGA AGTGGCTCCC
    AAGCCCCAAC CTCCAGATAC TCCAACAATC ATTCAACAAG GTGAACTGTC ACCTCAGCCT
    GTGATCCAGT CTAGTGAAGA TACCAATGGA AGTCCAACAT CTGCACAGGA CACACCCGTA
    CCATTGCCCA GAAAAATAAA TCCGATTAAG AGTAAAATGC GAAGGGTGAA GACTATTTAT
    GATTGCCAGG CAGACAATGA TGATGAGCTG ACTTTCGCTG AGGGTGAGGT CATTGTGGTA
    ACTGGTGAGG AAGATCAGGA GTGGTGGATT GGGCACATCG AAGGACAGCC GGAGAGAAAA
    GGTGTATTTC CCATGTCCTT TGTTCATATC TTATCAGATT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295587.2
    CDS170..3571
    Translation

    Target ORF information:

    RefSeq Version XM_009297312.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 1b (asap1b), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009297312.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGATGAGGT CATCATCCTC TCGTCTTAGT TTTTCCTCTA AGGAACCCAT ATGGAACAGG 
    ATGCCAGATC AAATATCGGT GTCGGAGTTT CTGTCGGAAA CAACAGAGGA TTACAACTCT
    CCCACTACAT CCAGCTTCAC CACCCGCTTG CAGAGCTGCA GGAATACAGT CAGCGTGTTA
    GAAGAGGCTT TGGATCAGGA CAGAACGTCT TTGCAGAAGG TCAAGAAGTC AGTCAAAGCC
    ATTTACAACT CTGGCCAAGA TCACATTCAA AATGAGGAAA CGTATTCCCA GGCATTGGAT
    AAATTTGGCA GCAACTTCAT CAGTCGGGAT AATCCAGATC TGGGCACAGC CTTTGTTAAA
    TTCTCCACTT TGACTAAAGA GCTTTCTGCC CTCCTCAAGA ACCTGCTTCA GAATTTAAGT
    CATAATGTGA TCTTTACACT GGACTCCCTG TTGAAAGGCG ACCTGAAAGG CGTGAAGGGG
    GACCTGAAAA AACCCTTTGA CAAGGCTTGG AAGGACTACG AAACCAAGTT TACAAAAATC
    GAAAAGGAAA AACGTGAGCA TGCCAAACAG CATGGAATGA TCCGAACAGA GATCACTGGT
    GCTGAGATCG CAGAGGAGAT GGAGAAAGAA AGGAGGCTCT TCCAGCTACA GATGTGTGAG
    TACCTGATCA AAGTAAATGA AATAAAGACC AAGAAAGGAG TTGACCTCCT TCAGAACTTG
    ATCAAGTATT ACCACGCGCA ATGCAACTTT TTCCAAGATG GCTTGAAGAC GGCAGATAAA
    CTGAAGCAGT ACATTGAGAA GTTAGCAGCT GATCTTTACA ATATAAAACA AACACAAGAT
    GAAGAGAAAA AGCAGCTCAC GGCTTTAAGA GATCTCATTA AATCCTCTCT ACAAGTGGAC
    CAAAAGGAGG ACTCTCAGAG CAAACAGGGT GGCTACAGTA TGCACCAGCT GCAGGGTAAC
    AAAGAGTTTG GCTGTGAGAA GAAAGGCTAT CTGTTAAAGA AGAGTGATGG GTTAAGGAAG
    GTGTGGCAGA GAAGGAAGTG CTCAGTTAAA GGCGGGATTC TCACCATCTC TCACGCCACT
    TCAAACAGGC AGCCTGTCAA ACTCAACCTG CTCACATGCC AGGTCAAGCC CAGTGCTGAG
    GACAGAAAGT GCTTCGATCT TATTTCCCAT AACCGCACAT ATCACTTTCA GGCAGAAGAT
    GAGCAGGAAT TTATAATATG GATCTCAGTG TTAACTAACA GCAAGGAGGA GGCACTTAAC
    ATGGCCTTCA GAGGAGAGCA GGGTGGAGGA GATGATGGGC TGGAGGATCT CACCAAAGCC
    ATAATCGATG ATGTGCTGCG CATGCCGGGA AATGAGTGGT GCTGTGATTG TGGGGCTCCA
    GATCCCAAAT GGTTGTCGAC TAACCTGGGA ATCCTGACAT GCATCGAATG TTCAGGCATA
    CACCGGGAGA TGGGCGTCCA CATCTCACGC ATCCAGTCTA TGGAGCTAGA CAAACTGGGA
    ACCTCCGAAC TCTTGCTGGC CAAGAATGTG GGGAACAGTA GTTTTAATGA TATAATGGAG
    GGAGGTCTTT CTTGTCCCTC TTCTAAACCC ACTCCCTCTA GTGACATGAC TGTTCGTAAG
    GAGTTCATCA ATGCTAAATA TGTAGATCAT AAGTTTGCAA GGAAAACCTG CAGCTCAGCA
    GAAGACAAGA GGAGTGATCT TTTTAAAGCC GTCGGGTCAC ATGACCTGCT AGCTCTCATC
    CAGGTGTATG CTGAGGGGGT AGAGCTTTTG GAACCACTAC ATGAAGGAGG ACAGGAAGGT
    GGAGAGACTG CATTGCACTA TGCCGTGAGG ACAGCTGACC AAACCTCACT GCACCTGGTC
    GACTTCCTTG TCCAGAACAG TGGGAATTTG GATAAGCAGA CGGAGCGAGG AAACACGGCC
    CTGCACTATT GCTGCCTGTA CGAGAAACAT GAATGCCTCA AACTACTGCT CCGAGGCAAA
    CCAGCTACTG ATATCACCAA TCAGAATGGG GACACAGCTT TGGATGTGGC TAGGCGGATG
    AAGCTCTTCC AGTGTGAGGA AGCATTGGTA CAAGCTGCTG CTGGAAAATT TAATACTAAA
    GTCCATGTGG AATATGAATG GAATCTCAGG TTGGAGGAGC TTGATGAAAG TGATGATGAT
    CTGGATTACA AACCAAGTCC CATTAAGAAG GATCGCTCAC CCCGTCCGCA GAGTTTCTGC
    CACTCATCCA GCCTCTCCCC TCATGATAAG CTCTCTCTGC CTGCTTTCAT TGGTCAGCGT
    GATAAGCAAC GCCTCTCCTA CTCTGCATTC ACCAATCAGA TGTACAGCGT TTCCACCGAC
    TGTCCGTCCT CTCCGGTTTC AGATGCTCCA CCTCTGCCGC CAAGGAATGC TGGCAAAGCC
    CCTCCATTGT CGAACCTGCC TCCCTCCACC CTTGCCCCTG CATCACAGAC CTCACCTAGT
    GGCAGCTCTA CCCTGTCAAA GAAGAGACCC CCTCCCCCTC CCCCTGGACA CAAACGCACG
    CTATCTGACC CCCCTACCCC TCTCTTTAAA GGAAGTGATT CAACGCCGCC TCCTGTCAAC
    AAGATGTCCC CTGTAACTAA TAGATTTGAG GGCATCCCAC AACAGCAAAG TCCCAACACC
    ACCAAGCCCA CACTTGGTCC AAGGGTCCTT CCCAAACTAC CTCAGAAGGT TGCCCTGCGT
    AAGATAGACA CCATACATCA CCCATCTGTG GACAAAACCA ACCAGCCTTC TGAAGCTGTC
    CTTTTTCAGA AAGTTCTGCA GAGTTCAGAT ACATCCCAGA AAGTTCCGAT AGCTGACAGG
    CCTCAGCCTG GAGACGTCGC TCCACAGCGA CCACAGCCTT CAGAATTACC ACCTAAACCA
    GGAGAACTGC CTCCAAAGCC TCAACTGTCC GAACTTCCCC CTAAACCCCA GTTGGGTGAC
    CTACCACCTA AACCTCAGCT TAAAGATCTC CCGCCCAAGC CCCAACTCGC AGACATCTCC
    CCAGCCAAAC CCATAACCAT TGAGGTCCCC CCAAAACCTT CTCCAGGAGA AGTGGCTCCC
    AAGCCCCAAC CTCCAGATAC TCCAACAATC ATTCAACAAG GTGAACTGTC ACCTCAGCCT
    GTGATCCAGT CTAGTGAAGA TACCAATGGA AGTCCAACAT CTGCACAGGA CACACCCGTA
    CCATTGCCCA GAAAAATAAA TCCGATTAAG AGTAAAATGC GAAGGGTGAA GACTATTTAT
    GATTGCCAGG CAGACAATGA TGATGAGCTG ACTTTCGCTG AGGGTGAGGT CATTGTGGTA
    ACTGGTGAGG AAGATCAGGA GTGGTGGATT GGGCACATCG AAGGACAGCC GGAGAGAAAA
    GGTGTATTTC CCATGTCCTT TGTTCATATC TTATCAGATT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa57693
    Clone ID Related Accession (Same CDS sequence) XM_017353941.1
    Accession Version XM_017353941.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3420bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCCAGATC AAATATCGGT GTCGGAGTTT CTGTCGGAAA CAACAGAGGA TTACAACTCT 
    CCCACTACAT CCAGCTTCAC CACCCGCTTG CAGAGCTGCA GGAATACAGT CAGCGTGTTA
    GAAGAGGCTT TGGATCAGGA CAGAACGTCT TTGCAGAAGG TCAAGAAGTC AGTCAAAGCC
    ATTTACAACT CTGGCCAAGA TCACATTCAA AATGAGGAAA CGTATTCCCA GGCATTGGAT
    AAATTTGGCA GCAACTTCAT CAGTCGGGAT AATCCAGATC TGGGCACAGC CTTTGTTAAA
    TTCTCCACTT TGACTAAAGA GCTTTCTGCC CTCCTCAAGA ACCTGCTTCA GAATTTAAGT
    CATAATGTGA TCTTTACACT GGACTCCCTG TTGAAAGGCG ACCTGAAAGG CGTGAAGGGG
    GACCTGAAAA AACCCTTTGA CAAGGCTTGG AAGGACTACG AAACCAAGTT TACAAAAATC
    GAAAAGGAAA AACGTGAGCA TGCCAAACAG CATGGAATGA TCCGAACAGA GATCACTGGT
    GCTGAGATCG CAGAGGAGAT GGAGAAAGAA AGGAGGCTCT TCCAGCTACA GATGTGTGAG
    TACCTGATCA AAGTAAATGA AATAAAGACC AAGAAAGGAG TTGACCTCCT TCAGAACTTG
    ATCAAGTATT ACCACGCGCA ATGCAACTTT TTCCAAGATG GCTTGAAGAC GGCAGATAAA
    CTGAAGCAGT ACATTGAGAA GTTAGCAGCT GATCTTTACA ATATAAAACA AACACAAGAT
    GAAGAGAAAA AGCAGCTCAC GGCTTTAAGA GATCTCATTA AATCCTCTCT ACAAGTGGAC
    CAAAAGGAGT CTAGAAGAGA CTCTCAGAGC AAACAGGGTG GCTACAGTAT GCACCAGCTG
    CAGGGTAACA AAGAGTTTGG CTGTGAGAAG AAAGGCTATC TGTTAAAGAA GAGTGATGGG
    TTAAGGAAGG TGTGGCAGAG AAGGAAGTGC TCAGTTAAAG GCGGGATTCT CACCATCTCT
    CACGCCACTT CAAACAGGCA GCCTGTCAAA CTCAACCTGC TCACATGCCA GGTCAAGCCC
    AGTGCTGAGG ACAGAAAGTG CTTCGATCTT ATTTCCCATA ACCGCACATA TCACTTTCAG
    GCAGAAGATG AGCAGGAATT TATAATATGG ATCTCAGTGT TAACTAACAG CAAGGAGGAG
    GCACTTAACA TGGCCTTCAG AGGAGAGCAG GGTGGAGGAG ATGATGGGCT GGAGGATCTC
    ACCAAAGCCA TAATCGATGA TGTGCTGCGC ATGCCGGGAA ATGAGTGGTG CTGTGATTGT
    GGGGCTCCAG ATCCCAAATG GTTGTCGACT AACCTGGGAA TCCTGACATG CATCGAATGT
    TCAGGCATAC ACCGGGAGAT GGGCGTCCAC ATCTCACGCA TCCAGTCTAT GGAGCTAGAC
    AAACTGGGAA CCTCCGAACT CTTGCTGGCC AAGAATGTGG GGAACAGTAG TTTTAATGAT
    ATAATGGAGG GAGGTCTTTC TTGTCCCTCT TCTAAACCCA CTCCCTCTAG TGACATGACT
    GTTCGTAAGG AGTTCATCAA TGCTAAATAT GTAGATCATA AGTTTGCAAG GAAAACCTGC
    AGCTCAGCAG AAGACAAGAG GAGTGATCTT TTTAAAGCCG TCGGGTCACA TGACCTGCTA
    GCTCTCATCC AGGTGTATGC TGAGGGGGTA GAGCTTTTGG AACCACTACA TGAAGGAGGA
    CAGGAAGGTG GAGAGACTGC ATTGCACTAT GCCGTGAGGA CAGCTGACCA AACCTCACTG
    CACCTGGTCG ACTTCCTTGT CCAGAACAGT GGGAATTTGG ATAAGCAGAC GGAGCGAGGA
    AACACGGCCC TGCACTATTG CTGCCTGTAC GAGAAACATG AATGCCTCAA ACTACTGCTC
    CGAGGCAAAC CAGCTACTGA TATCACCAAT CAGAATGGGG ACACAGCTTT GGATGTGGCT
    AGGCGGATGA AGCTCTTCCA GTGTGAGGAA GCATTGGTAC AAGCTGCTGC TGGAAAATTT
    AATACTAAAG TCCATGTGGA ATATGAATGG AATCTCAGGT TGGAGGAGCT TGATGAAAGT
    GATGATGATC TGGATTACAA ACCAAGTCCC ATTAAGAAGG ATCGCTCACC CCGTCCGCAG
    AGTTTCTGCC ACTCATCCAG CCTCTCCCCT CATGATAAGC TCTCTCTGCC TGCTTTCATT
    GGTCAGCGTG ATAAGCAACG CCTCTCCTAC TCTGCATTCA CCAATCAGAT GTACAGCGTT
    TCCACCGACT GTCCGTCCTC TCCGGTTTCA GATGCTCCAC CTCTGCCGCC AAGGAATGCT
    GGCAAAGCTC CATCTCTGGC TTTCCTTGGC TCTCATTCCC ACTATGCTAC TCTGGCTAGC
    ACTCGACCAT ACATCACCCC TCCATTGTCG AACCTGCCTC CCTCCACCCT TGCCCCTGCA
    TCACAGACCT CACCTAGTGG CAGCTCTACC CTGTCAAAGA AGAGACCCCC TCCCCCTCCC
    CCTGGACACA AACGCACGCT ATCTGACCCC CCTACCCCTC TCTTTAAAGG AAGTGATTCA
    ACGCCGCCTC CTGTCAACAA GATGTCCCCT GTAACTAATA GATTTGAGGG CATCCCACAA
    CAGCAAAGTC CCAACACCAC CAAGCCCACA CTTGGTCCAA GGGTCCTTCC CAAACTACCT
    CAGAAGGTTG CCCTGCGTAA GATAGACACC ATACATCACC CATCTGTGGA CAAAACCAAC
    CAGCCTTCTG AAGCTGTCCT TTTTCAGAAA GTTCTGCAGA GTTCAGATAC ATCCCAGAAA
    GTTCCGATAG CTGACAGGCC TCAGCCTGGA GACGTCGCTC CACAGCGACC ACAGCCTTCA
    GAATTACCAC CTAAACCAGG AGAACTGCCT CCAAAGCCTC AACTGTCCGA ACTTCCCCCT
    AAACCCCAGT TGGGTGACCT ACCACCTAAA CCTCAGCTTA AAGATCTCCC GCCCAAGCCC
    CAACTCGCAG ACATCTCCCC AGCCAAACCC ATAACCATTG AGGTCCCCCC AAAACCTTCT
    CCAGGAGAAG TGGCTCCCAA GCCCCAACCT CCAGATACTC CAACAATCAT TCAACAAGGT
    GAACTGTCAC CTCAGCCTGT GATCCAGTCT AGTGAAGATA CCAATGGAAG TCCAACATCT
    GCACAGGACA CACCCGTACC ATTGCCCAGA AAAATAAATC CGATTAAGAG TAAAATGCGA
    AGGGTGAAGA CTATTTATGA TTGCCAGGCA GACAATGATG ATGAGCTGAC TTTCGCTGAG
    GGTGAGGTCA TTGTGGTAAC TGGTGAGGAA GATCAGGAGT GGTGGATTGG GCACATCGAA
    GGACAGCCGG AGAGAAAAGG TGTATTTCCC ATGTCCTTTG TTCATATCTT ATCAGATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017209430.1
    CDS558..3977
    Translation

    Target ORF information:

    RefSeq Version XM_017353941.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 1b (asap1b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017353941.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCCAGATC AAATATCGGT GTCGGAGTTT CTGTCGGAAA CAACAGAGGA TTACAACTCT 
    CCCACTACAT CCAGCTTCAC CACCCGCTTG CAGAGCTGCA GGAATACAGT CAGCGTGTTA
    GAAGAGGCTT TGGATCAGGA CAGAACGTCT TTGCAGAAGG TCAAGAAGTC AGTCAAAGCC
    ATTTACAACT CTGGCCAAGA TCACATTCAA AATGAGGAAA CGTATTCCCA GGCATTGGAT
    AAATTTGGCA GCAACTTCAT CAGTCGGGAT AATCCAGATC TGGGCACAGC CTTTGTTAAA
    TTCTCCACTT TGACTAAAGA GCTTTCTGCC CTCCTCAAGA ACCTGCTTCA GAATTTAAGT
    CATAATGTGA TCTTTACACT GGACTCCCTG TTGAAAGGCG ACCTGAAAGG CGTGAAGGGG
    GACCTGAAAA AACCCTTTGA CAAGGCTTGG AAGGACTACG AAACCAAGTT TACAAAAATC
    GAAAAGGAAA AACGTGAGCA TGCCAAACAG CATGGAATGA TCCGAACAGA GATCACTGGT
    GCTGAGATCG CAGAGGAGAT GGAGAAAGAA AGGAGGCTCT TCCAGCTACA GATGTGTGAG
    TACCTGATCA AAGTAAATGA AATAAAGACC AAGAAAGGAG TTGACCTCCT TCAGAACTTG
    ATCAAGTATT ACCACGCGCA ATGCAACTTT TTCCAAGATG GCTTGAAGAC GGCAGATAAA
    CTGAAGCAGT ACATTGAGAA GTTAGCAGCT GATCTTTACA ATATAAAACA AACACAAGAT
    GAAGAGAAAA AGCAGCTCAC GGCTTTAAGA GATCTCATTA AATCCTCTCT ACAAGTGGAC
    CAAAAGGAGT CTAGAAGAGA CTCTCAGAGC AAACAGGGTG GCTACAGTAT GCACCAGCTG
    CAGGGTAACA AAGAGTTTGG CTGTGAGAAG AAAGGCTATC TGTTAAAGAA GAGTGATGGG
    TTAAGGAAGG TGTGGCAGAG AAGGAAGTGC TCAGTTAAAG GCGGGATTCT CACCATCTCT
    CACGCCACTT CAAACAGGCA GCCTGTCAAA CTCAACCTGC TCACATGCCA GGTCAAGCCC
    AGTGCTGAGG ACAGAAAGTG CTTCGATCTT ATTTCCCATA ACCGCACATA TCACTTTCAG
    GCAGAAGATG AGCAGGAATT TATAATATGG ATCTCAGTGT TAACTAACAG CAAGGAGGAG
    GCACTTAACA TGGCCTTCAG AGGAGAGCAG GGTGGAGGAG ATGATGGGCT GGAGGATCTC
    ACCAAAGCCA TAATCGATGA TGTGCTGCGC ATGCCGGGAA ATGAGTGGTG CTGTGATTGT
    GGGGCTCCAG ATCCCAAATG GTTGTCGACT AACCTGGGAA TCCTGACATG CATCGAATGT
    TCAGGCATAC ACCGGGAGAT GGGCGTCCAC ATCTCACGCA TCCAGTCTAT GGAGCTAGAC
    AAACTGGGAA CCTCCGAACT CTTGCTGGCC AAGAATGTGG GGAACAGTAG TTTTAATGAT
    ATAATGGAGG GAGGTCTTTC TTGTCCCTCT TCTAAACCCA CTCCCTCTAG TGACATGACT
    GTTCGTAAGG AGTTCATCAA TGCTAAATAT GTAGATCATA AGTTTGCAAG GAAAACCTGC
    AGCTCAGCAG AAGACAAGAG GAGTGATCTT TTTAAAGCCG TCGGGTCACA TGACCTGCTA
    GCTCTCATCC AGGTGTATGC TGAGGGGGTA GAGCTTTTGG AACCACTACA TGAAGGAGGA
    CAGGAAGGTG GAGAGACTGC ATTGCACTAT GCCGTGAGGA CAGCTGACCA AACCTCACTG
    CACCTGGTCG ACTTCCTTGT CCAGAACAGT GGGAATTTGG ATAAGCAGAC GGAGCGAGGA
    AACACGGCCC TGCACTATTG CTGCCTGTAC GAGAAACATG AATGCCTCAA ACTACTGCTC
    CGAGGCAAAC CAGCTACTGA TATCACCAAT CAGAATGGGG ACACAGCTTT GGATGTGGCT
    AGGCGGATGA AGCTCTTCCA GTGTGAGGAA GCATTGGTAC AAGCTGCTGC TGGAAAATTT
    AATACTAAAG TCCATGTGGA ATATGAATGG AATCTCAGGT TGGAGGAGCT TGATGAAAGT
    GATGATGATC TGGATTACAA ACCAAGTCCC ATTAAGAAGG ATCGCTCACC CCGTCCGCAG
    AGTTTCTGCC ACTCATCCAG CCTCTCCCCT CATGATAAGC TCTCTCTGCC TGCTTTCATT
    GGTCAGCGTG ATAAGCAACG CCTCTCCTAC TCTGCATTCA CCAATCAGAT GTACAGCGTT
    TCCACCGACT GTCCGTCCTC TCCGGTTTCA GATGCTCCAC CTCTGCCGCC AAGGAATGCT
    GGCAAAGCTC CATCTCTGGC TTTCCTTGGC TCTCATTCCC ACTATGCTAC TCTGGCTAGC
    ACTCGACCAT ACATCACCCC TCCATTGTCG AACCTGCCTC CCTCCACCCT TGCCCCTGCA
    TCACAGACCT CACCTAGTGG CAGCTCTACC CTGTCAAAGA AGAGACCCCC TCCCCCTCCC
    CCTGGACACA AACGCACGCT ATCTGACCCC CCTACCCCTC TCTTTAAAGG AAGTGATTCA
    ACGCCGCCTC CTGTCAACAA GATGTCCCCT GTAACTAATA GATTTGAGGG CATCCCACAA
    CAGCAAAGTC CCAACACCAC CAAGCCCACA CTTGGTCCAA GGGTCCTTCC CAAACTACCT
    CAGAAGGTTG CCCTGCGTAA GATAGACACC ATACATCACC CATCTGTGGA CAAAACCAAC
    CAGCCTTCTG AAGCTGTCCT TTTTCAGAAA GTTCTGCAGA GTTCAGATAC ATCCCAGAAA
    GTTCCGATAG CTGACAGGCC TCAGCCTGGA GACGTCGCTC CACAGCGACC ACAGCCTTCA
    GAATTACCAC CTAAACCAGG AGAACTGCCT CCAAAGCCTC AACTGTCCGA ACTTCCCCCT
    AAACCCCAGT TGGGTGACCT ACCACCTAAA CCTCAGCTTA AAGATCTCCC GCCCAAGCCC
    CAACTCGCAG ACATCTCCCC AGCCAAACCC ATAACCATTG AGGTCCCCCC AAAACCTTCT
    CCAGGAGAAG TGGCTCCCAA GCCCCAACCT CCAGATACTC CAACAATCAT TCAACAAGGT
    GAACTGTCAC CTCAGCCTGT GATCCAGTCT AGTGAAGATA CCAATGGAAG TCCAACATCT
    GCACAGGACA CACCCGTACC ATTGCCCAGA AAAATAAATC CGATTAAGAG TAAAATGCGA
    AGGGTGAAGA CTATTTATGA TTGCCAGGCA GACAATGATG ATGAGCTGAC TTTCGCTGAG
    GGTGAGGTCA TTGTGGTAAC TGGTGAGGAA GATCAGGAGT GGTGGATTGG GCACATCGAA
    GGACAGCCGG AGAGAAAAGG TGTATTTCCC ATGTCCTTTG TTCATATCTT ATCAGATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa57694
    Clone ID Related Accession (Same CDS sequence) XM_017353942.1
    Accession Version XM_017353942.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3414bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCCAGATC AAATATCGGT GTCGGAGTTT CTGTCGGAAA CAACAGAGGA TTACAACTCT 
    CCCACTACAT CCAGCTTCAC CACCCGCTTG CAGAGCTGCA GGAATACAGT CAGCGTGTTA
    GAAGAGGCTT TGGATCAGGA CAGAACGTCT TTGCAGAAGG TCAAGAAGTC AGTCAAAGCC
    ATTTACAACT CTGGCCAAGA TCACATTCAA AATGAGGAAA CGTATTCCCA GGCATTGGAT
    AAATTTGGCA GCAACTTCAT CAGTCGGGAT AATCCAGATC TGGGCACAGC CTTTGTTAAA
    TTCTCCACTT TGACTAAAGA GCTTTCTGCC CTCCTCAAGA ACCTGCTTCA GAATTTAAGT
    CATAATGTGA TCTTTACACT GGACTCCCTG TTGAAAGGCG ACCTGAAAGG CGTGAAGGGG
    GACCTGAAAA AACCCTTTGA CAAGGCTTGG AAGGACTACG AAACCAAGTT TACAAAAATC
    GAAAAGGAAA AACGTGAGCA TGCCAAACAG CATGGAATGA TCCGAACAGA GATCACTGGT
    GCTGAGATCG CAGAGGAGAT GGAGAAAGAA AGGAGGCTCT TCCAGCTACA GATGTGTGAG
    TACCTGATCA AAGTAAATGA AATAAAGACC AAGAAAGGAG TTGACCTCCT TCAGAACTTG
    ATCAAGTATT ACCACGCGCA ATGCAACTTT TTCCAAGATG GCTTGAAGAC GGCAGATAAA
    CTGAAGCAGT ACATTGAGAA GTTAGCAGCT GATCTTTACA ATATAAAACA AACACAAGAT
    GAAGAGAAAA AGCAGCTCAC GGCTTTAAGA GATCTCATTA AATCCTCTCT ACAAGTGGAC
    CAAAAGGAGC AGGACTCTCA GAGCAAACAG GGTGGCTACA GTATGCACCA GCTGCAGGGT
    AACAAAGAGT TTGGCTGTGA GAAGAAAGGC TATCTGTTAA AGAAGAGTGA TGGGTTAAGG
    AAGGTGTGGC AGAGAAGGAA GTGCTCAGTT AAAGGCGGGA TTCTCACCAT CTCTCACGCC
    ACTTCAAACA GGCAGCCTGT CAAACTCAAC CTGCTCACAT GCCAGGTCAA GCCCAGTGCT
    GAGGACAGAA AGTGCTTCGA TCTTATTTCC CATAACCGCA CATATCACTT TCAGGCAGAA
    GATGAGCAGG AATTTATAAT ATGGATCTCA GTGTTAACTA ACAGCAAGGA GGAGGCACTT
    AACATGGCCT TCAGAGGAGA GCAGGGTGGA GGAGATGATG GGCTGGAGGA TCTCACCAAA
    GCCATAATCG ATGATGTGCT GCGCATGCCG GGAAATGAGT GGTGCTGTGA TTGTGGGGCT
    CCAGATCCCA AATGGTTGTC GACTAACCTG GGAATCCTGA CATGCATCGA ATGTTCAGGC
    ATACACCGGG AGATGGGCGT CCACATCTCA CGCATCCAGT CTATGGAGCT AGACAAACTG
    GGAACCTCCG AACTCTTGCT GGCCAAGAAT GTGGGGAACA GTAGTTTTAA TGATATAATG
    GAGGGAGGTC TTTCTTGTCC CTCTTCTAAA CCCACTCCCT CTAGTGACAT GACTGTTCGT
    AAGGAGTTCA TCAATGCTAA ATATGTAGAT CATAAGTTTG CAAGGAAAAC CTGCAGCTCA
    GCAGAAGACA AGAGGAGTGA TCTTTTTAAA GCCGTCGGGT CACATGACCT GCTAGCTCTC
    ATCCAGGTGT ATGCTGAGGG GGTAGAGCTT TTGGAACCAC TACATGAAGG AGGACAGGAA
    GGTGGAGAGA CTGCATTGCA CTATGCCGTG AGGACAGCTG ACCAAACCTC ACTGCACCTG
    GTCGACTTCC TTGTCCAGAA CAGTGGGAAT TTGGATAAGC AGACGGAGCG AGGAAACACG
    GCCCTGCACT ATTGCTGCCT GTACGAGAAA CATGAATGCC TCAAACTACT GCTCCGAGGC
    AAACCAGCTA CTGATATCAC CAATCAGAAT GGGGACACAG CTTTGGATGT GGCTAGGCGG
    ATGAAGCTCT TCCAGTGTGA GGAAGCATTG GTACAAGCTG CTGCTGGAAA ATTTAATACT
    AAAGTCCATG TGGAATATGA ATGGAATCTC AGGTTGGAGG AGCTTGATGA AAGTGATGAT
    GATCTGGATT ACAAACCAAG TCCCATTAAG AAGGATCGCT CACCCCGTCC GCAGAGTTTC
    TGCCACTCAT CCAGCCTCTC CCCTCATGAT AAGCTCTCTC TGCCTGCTTT CATTGGTCAG
    CGTGATAAGC AACGCCTCTC CTACTCTGCA TTCACCAATC AGATGTACAG CGTTTCCACC
    GACTGTCCGT CCTCTCCGGT TTCAGATGCT CCACCTCTGC CGCCAAGGAA TGCTGGCAAA
    GCTCCATCTC TGGCTTTCCT TGGCTCTCAT TCCCACTATG CTACTCTGGC TAGCACTCGA
    CCATACATCA CCCCTCCATT GTCGAACCTG CCTCCCTCCA CCCTTGCCCC TGCATCACAG
    ACCTCACCTA GTGGCAGCTC TACCCTGTCA AAGAAGAGAC CCCCTCCCCC TCCCCCTGGA
    CACAAACGCA CGCTATCTGA CCCCCCTACC CCTCTCTTTA AAGGAAGTGA TTCAACGCCG
    CCTCCTGTCA ACAAGATGTC CCCTGTAACT AATAGATTTG AGGGCATCCC ACAACAGCAA
    AGTCCCAACA CCACCAAGCC CACACTTGGT CCAAGGGTCC TTCCCAAACT ACCTCAGAAG
    GTTGCCCTGC GTAAGATAGA CACCATACAT CACCCATCTG TGGACAAAAC CAACCAGCCT
    TCTGAAGCTG TCCTTTTTCA GAAAGTTCTG CAGAGTTCAG ATACATCCCA GAAAGTTCCG
    ATAGCTGACA GGCCTCAGCC TGGAGACGTC GCTCCACAGC GACCACAGCC TTCAGAATTA
    CCACCTAAAC CAGGAGAACT GCCTCCAAAG CCTCAACTGT CCGAACTTCC CCCTAAACCC
    CAGTTGGGTG ACCTACCACC TAAACCTCAG CTTAAAGATC TCCCGCCCAA GCCCCAACTC
    GCAGACATCT CCCCAGCCAA ACCCATAACC ATTGAGGTCC CCCCAAAACC TTCTCCAGGA
    GAAGTGGCTC CCAAGCCCCA ACCTCCAGAT ACTCCAACAA TCATTCAACA AGGTGAACTG
    TCACCTCAGC CTGTGATCCA GTCTAGTGAA GATACCAATG GAAGTCCAAC ATCTGCACAG
    GACACACCCG TACCATTGCC CAGAAAAATA AATCCGATTA AGAGTAAAAT GCGAAGGGTG
    AAGACTATTT ATGATTGCCA GGCAGACAAT GATGATGAGC TGACTTTCGC TGAGGGTGAG
    GTCATTGTGG TAACTGGTGA GGAAGATCAG GAGTGGTGGA TTGGGCACAT CGAAGGACAG
    CCGGAGAGAA AAGGTGTATT TCCCATGTCC TTTGTTCATA TCTTATCAGA TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017209431.1
    CDS558..3971
    Translation

    Target ORF information:

    RefSeq Version XM_017353942.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 1b (asap1b), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017353942.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCCAGATC AAATATCGGT GTCGGAGTTT CTGTCGGAAA CAACAGAGGA TTACAACTCT 
    CCCACTACAT CCAGCTTCAC CACCCGCTTG CAGAGCTGCA GGAATACAGT CAGCGTGTTA
    GAAGAGGCTT TGGATCAGGA CAGAACGTCT TTGCAGAAGG TCAAGAAGTC AGTCAAAGCC
    ATTTACAACT CTGGCCAAGA TCACATTCAA AATGAGGAAA CGTATTCCCA GGCATTGGAT
    AAATTTGGCA GCAACTTCAT CAGTCGGGAT AATCCAGATC TGGGCACAGC CTTTGTTAAA
    TTCTCCACTT TGACTAAAGA GCTTTCTGCC CTCCTCAAGA ACCTGCTTCA GAATTTAAGT
    CATAATGTGA TCTTTACACT GGACTCCCTG TTGAAAGGCG ACCTGAAAGG CGTGAAGGGG
    GACCTGAAAA AACCCTTTGA CAAGGCTTGG AAGGACTACG AAACCAAGTT TACAAAAATC
    GAAAAGGAAA AACGTGAGCA TGCCAAACAG CATGGAATGA TCCGAACAGA GATCACTGGT
    GCTGAGATCG CAGAGGAGAT GGAGAAAGAA AGGAGGCTCT TCCAGCTACA GATGTGTGAG
    TACCTGATCA AAGTAAATGA AATAAAGACC AAGAAAGGAG TTGACCTCCT TCAGAACTTG
    ATCAAGTATT ACCACGCGCA ATGCAACTTT TTCCAAGATG GCTTGAAGAC GGCAGATAAA
    CTGAAGCAGT ACATTGAGAA GTTAGCAGCT GATCTTTACA ATATAAAACA AACACAAGAT
    GAAGAGAAAA AGCAGCTCAC GGCTTTAAGA GATCTCATTA AATCCTCTCT ACAAGTGGAC
    CAAAAGGAGC AGGACTCTCA GAGCAAACAG GGTGGCTACA GTATGCACCA GCTGCAGGGT
    AACAAAGAGT TTGGCTGTGA GAAGAAAGGC TATCTGTTAA AGAAGAGTGA TGGGTTAAGG
    AAGGTGTGGC AGAGAAGGAA GTGCTCAGTT AAAGGCGGGA TTCTCACCAT CTCTCACGCC
    ACTTCAAACA GGCAGCCTGT CAAACTCAAC CTGCTCACAT GCCAGGTCAA GCCCAGTGCT
    GAGGACAGAA AGTGCTTCGA TCTTATTTCC CATAACCGCA CATATCACTT TCAGGCAGAA
    GATGAGCAGG AATTTATAAT ATGGATCTCA GTGTTAACTA ACAGCAAGGA GGAGGCACTT
    AACATGGCCT TCAGAGGAGA GCAGGGTGGA GGAGATGATG GGCTGGAGGA TCTCACCAAA
    GCCATAATCG ATGATGTGCT GCGCATGCCG GGAAATGAGT GGTGCTGTGA TTGTGGGGCT
    CCAGATCCCA AATGGTTGTC GACTAACCTG GGAATCCTGA CATGCATCGA ATGTTCAGGC
    ATACACCGGG AGATGGGCGT CCACATCTCA CGCATCCAGT CTATGGAGCT AGACAAACTG
    GGAACCTCCG AACTCTTGCT GGCCAAGAAT GTGGGGAACA GTAGTTTTAA TGATATAATG
    GAGGGAGGTC TTTCTTGTCC CTCTTCTAAA CCCACTCCCT CTAGTGACAT GACTGTTCGT
    AAGGAGTTCA TCAATGCTAA ATATGTAGAT CATAAGTTTG CAAGGAAAAC CTGCAGCTCA
    GCAGAAGACA AGAGGAGTGA TCTTTTTAAA GCCGTCGGGT CACATGACCT GCTAGCTCTC
    ATCCAGGTGT ATGCTGAGGG GGTAGAGCTT TTGGAACCAC TACATGAAGG AGGACAGGAA
    GGTGGAGAGA CTGCATTGCA CTATGCCGTG AGGACAGCTG ACCAAACCTC ACTGCACCTG
    GTCGACTTCC TTGTCCAGAA CAGTGGGAAT TTGGATAAGC AGACGGAGCG AGGAAACACG
    GCCCTGCACT ATTGCTGCCT GTACGAGAAA CATGAATGCC TCAAACTACT GCTCCGAGGC
    AAACCAGCTA CTGATATCAC CAATCAGAAT GGGGACACAG CTTTGGATGT GGCTAGGCGG
    ATGAAGCTCT TCCAGTGTGA GGAAGCATTG GTACAAGCTG CTGCTGGAAA ATTTAATACT
    AAAGTCCATG TGGAATATGA ATGGAATCTC AGGTTGGAGG AGCTTGATGA AAGTGATGAT
    GATCTGGATT ACAAACCAAG TCCCATTAAG AAGGATCGCT CACCCCGTCC GCAGAGTTTC
    TGCCACTCAT CCAGCCTCTC CCCTCATGAT AAGCTCTCTC TGCCTGCTTT CATTGGTCAG
    CGTGATAAGC AACGCCTCTC CTACTCTGCA TTCACCAATC AGATGTACAG CGTTTCCACC
    GACTGTCCGT CCTCTCCGGT TTCAGATGCT CCACCTCTGC CGCCAAGGAA TGCTGGCAAA
    GCTCCATCTC TGGCTTTCCT TGGCTCTCAT TCCCACTATG CTACTCTGGC TAGCACTCGA
    CCATACATCA CCCCTCCATT GTCGAACCTG CCTCCCTCCA CCCTTGCCCC TGCATCACAG
    ACCTCACCTA GTGGCAGCTC TACCCTGTCA AAGAAGAGAC CCCCTCCCCC TCCCCCTGGA
    CACAAACGCA CGCTATCTGA CCCCCCTACC CCTCTCTTTA AAGGAAGTGA TTCAACGCCG
    CCTCCTGTCA ACAAGATGTC CCCTGTAACT AATAGATTTG AGGGCATCCC ACAACAGCAA
    AGTCCCAACA CCACCAAGCC CACACTTGGT CCAAGGGTCC TTCCCAAACT ACCTCAGAAG
    GTTGCCCTGC GTAAGATAGA CACCATACAT CACCCATCTG TGGACAAAAC CAACCAGCCT
    TCTGAAGCTG TCCTTTTTCA GAAAGTTCTG CAGAGTTCAG ATACATCCCA GAAAGTTCCG
    ATAGCTGACA GGCCTCAGCC TGGAGACGTC GCTCCACAGC GACCACAGCC TTCAGAATTA
    CCACCTAAAC CAGGAGAACT GCCTCCAAAG CCTCAACTGT CCGAACTTCC CCCTAAACCC
    CAGTTGGGTG ACCTACCACC TAAACCTCAG CTTAAAGATC TCCCGCCCAA GCCCCAACTC
    GCAGACATCT CCCCAGCCAA ACCCATAACC ATTGAGGTCC CCCCAAAACC TTCTCCAGGA
    GAAGTGGCTC CCAAGCCCCA ACCTCCAGAT ACTCCAACAA TCATTCAACA AGGTGAACTG
    TCACCTCAGC CTGTGATCCA GTCTAGTGAA GATACCAATG GAAGTCCAAC ATCTGCACAG
    GACACACCCG TACCATTGCC CAGAAAAATA AATCCGATTA AGAGTAAAAT GCGAAGGGTG
    AAGACTATTT ATGATTGCCA GGCAGACAAT GATGATGAGC TGACTTTCGC TGAGGGTGAG
    GTCATTGTGG TAACTGGTGA GGAAGATCAG GAGTGGTGGA TTGGGCACAT CGAAGGACAG
    CCGGAGAGAA AAGGTGTATT TCCCATGTCC TTTGTTCATA TCTTATCAGA TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa57695
    Clone ID Related Accession (Same CDS sequence) XM_017353943.1
    Accession Version XM_017353943.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3411bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCCAGATC AAATATCGGT GTCGGAGTTT CTGTCGGAAA CAACAGAGGA TTACAACTCT 
    CCCACTACAT CCAGCTTCAC CACCCGCTTG CAGAGCTGCA GGAATACAGT CAGCGTGTTA
    GAAGAGGCTT TGGATCAGGA CAGAACGTCT TTGCAGAAGG TCAAGAAGTC AGTCAAAGCC
    ATTTACAACT CTGGCCAAGA TCACATTCAA AATGAGGAAA CGTATTCCCA GGCATTGGAT
    AAATTTGGCA GCAACTTCAT CAGTCGGGAT AATCCAGATC TGGGCACAGC CTTTGTTAAA
    TTCTCCACTT TGACTAAAGA GCTTTCTGCC CTCCTCAAGA ACCTGCTTCA GAATTTAAGT
    CATAATGTGA TCTTTACACT GGACTCCCTG TTGAAAGGCG ACCTGAAAGG CGTGAAGGGG
    GACCTGAAAA AACCCTTTGA CAAGGCTTGG AAGGACTACG AAACCAAGTT TACAAAAATC
    GAAAAGGAAA AACGTGAGCA TGCCAAACAG CATGGAATGA TCCGAACAGA GATCACTGGT
    GCTGAGATCG CAGAGGAGAT GGAGAAAGAA AGGAGGCTCT TCCAGCTACA GATGTGTGAG
    TACCTGATCA AAGTAAATGA AATAAAGACC AAGAAAGGAG TTGACCTCCT TCAGAACTTG
    ATCAAGTATT ACCACGCGCA ATGCAACTTT TTCCAAGATG GCTTGAAGAC GGCAGATAAA
    CTGAAGCAGT ACATTGAGAA GTTAGCAGCT GATCTTTACA ATATAAAACA AACACAAGAT
    GAAGAGAAAA AGCAGCTCAC GGCTTTAAGA GATCTCATTA AATCCTCTCT ACAAGTGGAC
    CAAAAGGAGG ACTCTCAGAG CAAACAGGGT GGCTACAGTA TGCACCAGCT GCAGGGTAAC
    AAAGAGTTTG GCTGTGAGAA GAAAGGCTAT CTGTTAAAGA AGAGTGATGG GTTAAGGAAG
    GTGTGGCAGA GAAGGAAGTG CTCAGTTAAA GGCGGGATTC TCACCATCTC TCACGCCACT
    TCAAACAGGC AGCCTGTCAA ACTCAACCTG CTCACATGCC AGGTCAAGCC CAGTGCTGAG
    GACAGAAAGT GCTTCGATCT TATTTCCCAT AACCGCACAT ATCACTTTCA GGCAGAAGAT
    GAGCAGGAAT TTATAATATG GATCTCAGTG TTAACTAACA GCAAGGAGGA GGCACTTAAC
    ATGGCCTTCA GAGGAGAGCA GGGTGGAGGA GATGATGGGC TGGAGGATCT CACCAAAGCC
    ATAATCGATG ATGTGCTGCG CATGCCGGGA AATGAGTGGT GCTGTGATTG TGGGGCTCCA
    GATCCCAAAT GGTTGTCGAC TAACCTGGGA ATCCTGACAT GCATCGAATG TTCAGGCATA
    CACCGGGAGA TGGGCGTCCA CATCTCACGC ATCCAGTCTA TGGAGCTAGA CAAACTGGGA
    ACCTCCGAAC TCTTGCTGGC CAAGAATGTG GGGAACAGTA GTTTTAATGA TATAATGGAG
    GGAGGTCTTT CTTGTCCCTC TTCTAAACCC ACTCCCTCTA GTGACATGAC TGTTCGTAAG
    GAGTTCATCA ATGCTAAATA TGTAGATCAT AAGTTTGCAA GGAAAACCTG CAGCTCAGCA
    GAAGACAAGA GGAGTGATCT TTTTAAAGCC GTCGGGTCAC ATGACCTGCT AGCTCTCATC
    CAGGTGTATG CTGAGGGGGT AGAGCTTTTG GAACCACTAC ATGAAGGAGG ACAGGAAGGT
    GGAGAGACTG CATTGCACTA TGCCGTGAGG ACAGCTGACC AAACCTCACT GCACCTGGTC
    GACTTCCTTG TCCAGAACAG TGGGAATTTG GATAAGCAGA CGGAGCGAGG AAACACGGCC
    CTGCACTATT GCTGCCTGTA CGAGAAACAT GAATGCCTCA AACTACTGCT CCGAGGCAAA
    CCAGCTACTG ATATCACCAA TCAGAATGGG GACACAGCTT TGGATGTGGC TAGGCGGATG
    AAGCTCTTCC AGTGTGAGGA AGCATTGGTA CAAGCTGCTG CTGGAAAATT TAATACTAAA
    GTCCATGTGG AATATGAATG GAATCTCAGG TTGGAGGAGC TTGATGAAAG TGATGATGAT
    CTGGATTACA AACCAAGTCC CATTAAGAAG GATCGCTCAC CCCGTCCGCA GAGTTTCTGC
    CACTCATCCA GCCTCTCCCC TCATGATAAG CTCTCTCTGC CTGCTTTCAT TGGTCAGCGT
    GATAAGCAAC GCCTCTCCTA CTCTGCATTC ACCAATCAGA TGTACAGCGT TTCCACCGAC
    TGTCCGTCCT CTCCGGTTTC AGATGCTCCA CCTCTGCCGC CAAGGAATGC TGGCAAAGCT
    CCATCTCTGG CTTTCCTTGG CTCTCATTCC CACTATGCTA CTCTGGCTAG CACTCGACCA
    TACATCACCC CTCCATTGTC GAACCTGCCT CCCTCCACCC TTGCCCCTGC ATCACAGACC
    TCACCTAGTG GCAGCTCTAC CCTGTCAAAG AAGAGACCCC CTCCCCCTCC CCCTGGACAC
    AAACGCACGC TATCTGACCC CCCTACCCCT CTCTTTAAAG GAAGTGATTC AACGCCGCCT
    CCTGTCAACA AGATGTCCCC TGTAACTAAT AGATTTGAGG GCATCCCACA ACAGCAAAGT
    CCCAACACCA CCAAGCCCAC ACTTGGTCCA AGGGTCCTTC CCAAACTACC TCAGAAGGTT
    GCCCTGCGTA AGATAGACAC CATACATCAC CCATCTGTGG ACAAAACCAA CCAGCCTTCT
    GAAGCTGTCC TTTTTCAGAA AGTTCTGCAG AGTTCAGATA CATCCCAGAA AGTTCCGATA
    GCTGACAGGC CTCAGCCTGG AGACGTCGCT CCACAGCGAC CACAGCCTTC AGAATTACCA
    CCTAAACCAG GAGAACTGCC TCCAAAGCCT CAACTGTCCG AACTTCCCCC TAAACCCCAG
    TTGGGTGACC TACCACCTAA ACCTCAGCTT AAAGATCTCC CGCCCAAGCC CCAACTCGCA
    GACATCTCCC CAGCCAAACC CATAACCATT GAGGTCCCCC CAAAACCTTC TCCAGGAGAA
    GTGGCTCCCA AGCCCCAACC TCCAGATACT CCAACAATCA TTCAACAAGG TGAACTGTCA
    CCTCAGCCTG TGATCCAGTC TAGTGAAGAT ACCAATGGAA GTCCAACATC TGCACAGGAC
    ACACCCGTAC CATTGCCCAG AAAAATAAAT CCGATTAAGA GTAAAATGCG AAGGGTGAAG
    ACTATTTATG ATTGCCAGGC AGACAATGAT GATGAGCTGA CTTTCGCTGA GGGTGAGGTC
    ATTGTGGTAA CTGGTGAGGA AGATCAGGAG TGGTGGATTG GGCACATCGA AGGACAGCCG
    GAGAGAAAAG GTGTATTTCC CATGTCCTTT GTTCATATCT TATCAGATTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017209432.1
    CDS558..3968
    Translation

    Target ORF information:

    RefSeq Version XM_017353943.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 1b (asap1b), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017353943.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCCAGATC AAATATCGGT GTCGGAGTTT CTGTCGGAAA CAACAGAGGA TTACAACTCT 
    CCCACTACAT CCAGCTTCAC CACCCGCTTG CAGAGCTGCA GGAATACAGT CAGCGTGTTA
    GAAGAGGCTT TGGATCAGGA CAGAACGTCT TTGCAGAAGG TCAAGAAGTC AGTCAAAGCC
    ATTTACAACT CTGGCCAAGA TCACATTCAA AATGAGGAAA CGTATTCCCA GGCATTGGAT
    AAATTTGGCA GCAACTTCAT CAGTCGGGAT AATCCAGATC TGGGCACAGC CTTTGTTAAA
    TTCTCCACTT TGACTAAAGA GCTTTCTGCC CTCCTCAAGA ACCTGCTTCA GAATTTAAGT
    CATAATGTGA TCTTTACACT GGACTCCCTG TTGAAAGGCG ACCTGAAAGG CGTGAAGGGG
    GACCTGAAAA AACCCTTTGA CAAGGCTTGG AAGGACTACG AAACCAAGTT TACAAAAATC
    GAAAAGGAAA AACGTGAGCA TGCCAAACAG CATGGAATGA TCCGAACAGA GATCACTGGT
    GCTGAGATCG CAGAGGAGAT GGAGAAAGAA AGGAGGCTCT TCCAGCTACA GATGTGTGAG
    TACCTGATCA AAGTAAATGA AATAAAGACC AAGAAAGGAG TTGACCTCCT TCAGAACTTG
    ATCAAGTATT ACCACGCGCA ATGCAACTTT TTCCAAGATG GCTTGAAGAC GGCAGATAAA
    CTGAAGCAGT ACATTGAGAA GTTAGCAGCT GATCTTTACA ATATAAAACA AACACAAGAT
    GAAGAGAAAA AGCAGCTCAC GGCTTTAAGA GATCTCATTA AATCCTCTCT ACAAGTGGAC
    CAAAAGGAGG ACTCTCAGAG CAAACAGGGT GGCTACAGTA TGCACCAGCT GCAGGGTAAC
    AAAGAGTTTG GCTGTGAGAA GAAAGGCTAT CTGTTAAAGA AGAGTGATGG GTTAAGGAAG
    GTGTGGCAGA GAAGGAAGTG CTCAGTTAAA GGCGGGATTC TCACCATCTC TCACGCCACT
    TCAAACAGGC AGCCTGTCAA ACTCAACCTG CTCACATGCC AGGTCAAGCC CAGTGCTGAG
    GACAGAAAGT GCTTCGATCT TATTTCCCAT AACCGCACAT ATCACTTTCA GGCAGAAGAT
    GAGCAGGAAT TTATAATATG GATCTCAGTG TTAACTAACA GCAAGGAGGA GGCACTTAAC
    ATGGCCTTCA GAGGAGAGCA GGGTGGAGGA GATGATGGGC TGGAGGATCT CACCAAAGCC
    ATAATCGATG ATGTGCTGCG CATGCCGGGA AATGAGTGGT GCTGTGATTG TGGGGCTCCA
    GATCCCAAAT GGTTGTCGAC TAACCTGGGA ATCCTGACAT GCATCGAATG TTCAGGCATA
    CACCGGGAGA TGGGCGTCCA CATCTCACGC ATCCAGTCTA TGGAGCTAGA CAAACTGGGA
    ACCTCCGAAC TCTTGCTGGC CAAGAATGTG GGGAACAGTA GTTTTAATGA TATAATGGAG
    GGAGGTCTTT CTTGTCCCTC TTCTAAACCC ACTCCCTCTA GTGACATGAC TGTTCGTAAG
    GAGTTCATCA ATGCTAAATA TGTAGATCAT AAGTTTGCAA GGAAAACCTG CAGCTCAGCA
    GAAGACAAGA GGAGTGATCT TTTTAAAGCC GTCGGGTCAC ATGACCTGCT AGCTCTCATC
    CAGGTGTATG CTGAGGGGGT AGAGCTTTTG GAACCACTAC ATGAAGGAGG ACAGGAAGGT
    GGAGAGACTG CATTGCACTA TGCCGTGAGG ACAGCTGACC AAACCTCACT GCACCTGGTC
    GACTTCCTTG TCCAGAACAG TGGGAATTTG GATAAGCAGA CGGAGCGAGG AAACACGGCC
    CTGCACTATT GCTGCCTGTA CGAGAAACAT GAATGCCTCA AACTACTGCT CCGAGGCAAA
    CCAGCTACTG ATATCACCAA TCAGAATGGG GACACAGCTT TGGATGTGGC TAGGCGGATG
    AAGCTCTTCC AGTGTGAGGA AGCATTGGTA CAAGCTGCTG CTGGAAAATT TAATACTAAA
    GTCCATGTGG AATATGAATG GAATCTCAGG TTGGAGGAGC TTGATGAAAG TGATGATGAT
    CTGGATTACA AACCAAGTCC CATTAAGAAG GATCGCTCAC CCCGTCCGCA GAGTTTCTGC
    CACTCATCCA GCCTCTCCCC TCATGATAAG CTCTCTCTGC CTGCTTTCAT TGGTCAGCGT
    GATAAGCAAC GCCTCTCCTA CTCTGCATTC ACCAATCAGA TGTACAGCGT TTCCACCGAC
    TGTCCGTCCT CTCCGGTTTC AGATGCTCCA CCTCTGCCGC CAAGGAATGC TGGCAAAGCT
    CCATCTCTGG CTTTCCTTGG CTCTCATTCC CACTATGCTA CTCTGGCTAG CACTCGACCA
    TACATCACCC CTCCATTGTC GAACCTGCCT CCCTCCACCC TTGCCCCTGC ATCACAGACC
    TCACCTAGTG GCAGCTCTAC CCTGTCAAAG AAGAGACCCC CTCCCCCTCC CCCTGGACAC
    AAACGCACGC TATCTGACCC CCCTACCCCT CTCTTTAAAG GAAGTGATTC AACGCCGCCT
    CCTGTCAACA AGATGTCCCC TGTAACTAAT AGATTTGAGG GCATCCCACA ACAGCAAAGT
    CCCAACACCA CCAAGCCCAC ACTTGGTCCA AGGGTCCTTC CCAAACTACC TCAGAAGGTT
    GCCCTGCGTA AGATAGACAC CATACATCAC CCATCTGTGG ACAAAACCAA CCAGCCTTCT
    GAAGCTGTCC TTTTTCAGAA AGTTCTGCAG AGTTCAGATA CATCCCAGAA AGTTCCGATA
    GCTGACAGGC CTCAGCCTGG AGACGTCGCT CCACAGCGAC CACAGCCTTC AGAATTACCA
    CCTAAACCAG GAGAACTGCC TCCAAAGCCT CAACTGTCCG AACTTCCCCC TAAACCCCAG
    TTGGGTGACC TACCACCTAA ACCTCAGCTT AAAGATCTCC CGCCCAAGCC CCAACTCGCA
    GACATCTCCC CAGCCAAACC CATAACCATT GAGGTCCCCC CAAAACCTTC TCCAGGAGAA
    GTGGCTCCCA AGCCCCAACC TCCAGATACT CCAACAATCA TTCAACAAGG TGAACTGTCA
    CCTCAGCCTG TGATCCAGTC TAGTGAAGAT ACCAATGGAA GTCCAACATC TGCACAGGAC
    ACACCCGTAC CATTGCCCAG AAAAATAAAT CCGATTAAGA GTAAAATGCG AAGGGTGAAG
    ACTATTTATG ATTGCCAGGC AGACAATGAT GATGAGCTGA CTTTCGCTGA GGGTGAGGTC
    ATTGTGGTAA CTGGTGAGGA AGATCAGGAG TGGTGGATTG GGCACATCGA AGGACAGCCG
    GAGAGAAAAG GTGTATTTCC CATGTCCTTT GTTCATATCT TATCAGATTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa28402
    Clone ID Related Accession (Same CDS sequence) XM_005162605.3
    Accession Version XM_005162605.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3270bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005162605.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCCAGATC AAATATCGGT GTCGGAGTTT CTGTCGGAAA CAACAGAGGA TTACAACTCT 
    CCCACTACAT CCAGCTTCAC CACCCGCTTG CAGAGCTGCA GGAATACAGT CAGCGTGTTA
    GAAGAGGCTT TGGATCAGGA CAGAACGTCT TTGCAGAAGG TCAAGAAGTC AGTCAAAGCC
    ATTTACAACT CTGGCCAAGA TCACATTCAA AATGAGGAAA CGTATTCCCA GGCATTGGAT
    AAATTTGGCA GCAACTTCAT CAGTCGGGAT AATCCAGATC TGGGCACAGC CTTTGTTAAA
    TTCTCCACTT TGACTAAAGA GCTTTCTGCC CTCCTCAAGA ACCTGCTTCA GAATTTAAGT
    CATAATGTGA TCTTTACACT GGACTCCCTG TTGAAAGGCG ACCTGAAAGG CGTGAAGGGG
    GACCTGAAAA AACCCTTTGA CAAGGCTTGG AAGGACTACG AAACCAAGTT TACAAAAATC
    GAAAAGGAAA AACGTGAGCA TGCCAAACAG CATGGAATGA TCCGAACAGA GATCACTGGT
    GCTGAGATCG CAGAGGAGAT GGAGAAAGAA AGGAGGCTCT TCCAGCTACA GATGTGTGAG
    TACCTGATCA AAGTAAATGA AATAAAGACC AAGAAAGGAG TTGACCTCCT TCAGAACTTG
    ATCAAGTATT ACCACGCGCA ATGCAACTTT TTCCAAGATG GCTTGAAGAC GGCAGATAAA
    CTGAAGCAGT ACATTGAGAA GTTAGCAGCT GATCTTTACA ATATAAAACA AACACAAGAT
    GAAGAGAAAA AGCAGCTCAC GGCTTTAAGA GATCTCATTA AATCCTCTCT ACAAGTGGAC
    CAAAAGGAGT CTAGAAGACA GGACTCTCAG AGCAAACAGG GTGGCTACAG TATGCACCAG
    CTGCAGGGTA ACAAAGAGTT TGGCTGTGAG AAGAAAGGCT ATCTGTTAAA GAAGAGTGAT
    GGGTTAAGGA AGGTGTGGCA GAGAAGGAAG TGCTCAGTTA AAGGCGGGAT TCTCACCATC
    TCTCACGCCA CTTCAAACAG GCAGCCTGTC AAACTCAACC TGCTCACATG CCAGGTCAAG
    CCCAGTGCTG AGGACAGAAA GTGCTTCGAT CTTATTTCCC ATAACCGCAC ATATCACTTT
    CAGGCAGAAG ATGAGCAGGA ATTTATAATA TGGATCTCAG TGTTAACTAA CAGCAAGGAG
    GAGGCACTTA ACATGGCCTT CAGAGGAGAG CAGGGTGGAG GAGATGATGG GCTGGAGGAT
    CTCACCAAAG CCATAATCGA TGATGTGCTG CGCATGCCGG GAAATGAGTG GTGCTGTGAT
    TGTGGGGCTC CAGATCCCAA ATGGTTGTCG ACTAACCTGG GAATCCTGAC ATGCATCGAA
    TGTTCAGGCA TACACCGGGA GATGGGCGTC CACATCTCAC GCATCCAGTC TATGGAGCTA
    GACAAACTGG GAACCTCCGA ACTCTTGCTG GCCAAGAATG TGGGGAACAG TAGTTTTAAT
    GATATAATGG AGGGAGGTCT TTCTTGTCCC TCTTCTAAAC CCACTCCCTC TAGTGACATG
    ACTGTTCGTA AGGAGTTCAT CAATGCTAAA TATGTAGATC ATAAGTTTGC AAGGAAAACC
    TGCAGCTCAG CAGAAGACAA GAGGAGTGAT CTTTTTAAAG CCGTCGGGTC ACATGACCTG
    CTAGCTCTCA TCCAGGTGTA TGCTGAGGGG GTAGAGCTTT TGGAACCACT ACATGAAGGA
    GGACAGGAAG GTGGAGAGAC TGCATTGCAC TATGCCGTGA GGACAGCTGA CCAAACCTCA
    CTGCACCTGG TCGACTTCCT TGTCCAGAAC AGTGGGAATT TGGATAAGCA GACGGAGCGA
    GGAAACACGG CCCTGCACTA TTGCTGCCTG TACGAGAAAC ATGAATGCCT CAAACTACTG
    CTCCGAGGCA AACCAGCTAC TGATATCACC AATCAGAATG GGGACACAGC TTTGGATGTG
    GCTAGGCGGA TGAAGCTCTT CCAGTGTGAG GAAGCATTGG TACAAGCTGC TGCTGGAAAA
    TTTAATACTA AAGTCCATGT GGAATATGAA TGGAATCTCA GGTTGGAGGA GCTTGATGAA
    AGTGATGATG ATCTGGATTA CAAACCAAGT CCCATTAAGA AGGATCGCTC ACCCCGTCCG
    CAGAGTTTCT GCCACTCATC CAGCCTCTCC CCTCATGATA AGCTCTCTCT GCCTGCTTTC
    ATTGGTCAGC GTGATAAGCA ACGCCTCTCC TACTCTGCAT TCACCAATCA GATGTACAGC
    GTTTCCACCG ACTGTCCGTC CTCTCCGGTT TCAGATGCTC CACCTCTGCC GCCAAGGAAT
    GCTGGCAAAG CTCCATCTCT GGCTTTCCTT GGCTCTCATT CCCACTATGC TACTCTGGCT
    AGCACTCGAC CATACATCAG AAGTGATTCA ACGCCGCCTC CTGTCAACAA GATGTCCCCT
    GTAACTAATA GATTTGAGGG CATCCCACAA CAGCAAAGTC CCAACACCAC CAAGCCCACA
    CTTGGTCCAA GGGTCCTTCC CAAACTACCT CAGAAGGTTG CCCTGCGTAA GATAGACACC
    ATACATCACC CATCTGTGGA CAAAACCAAC CAGCCTTCTG AAGCTGTCCT TTTTCAGAAA
    GTTCTGCAGA GTTCAGATAC ATCCCAGAAA GTTCCGATAG CTGACAGGCC TCAGCCTGGA
    GACGTCGCTC CACAGCGACC ACAGCCTTCA GAATTACCAC CTAAACCAGG AGAACTGCCT
    CCAAAGCCTC AACTGTCCGA ACTTCCCCCT AAACCCCAGT TGGGTGACCT ACCACCTAAA
    CCTCAGCTTA AAGATCTCCC GCCCAAGCCC CAACTCGCAG ACATCTCCCC AGCCAAACCC
    ATAACCATTG AGGTCCCCCC AAAACCTTCT CCAGGAGAAG TGGCTCCCAA GCCCCAACCT
    CCAGATACTC CAACAATCAT TCAACAAGGT GAACTGTCAC CTCAGCCTGT GATCCAGTCT
    AGTGAAGATA CCAATGGAAG TCCAACATCT GCACAGGACA CACCCGTACC ATTGCCCAGA
    AAAATAAATC CGATTAAGAG TAAAATGCGA AGGGTGAAGA CTATTTATGA TTGCCAGGCA
    GACAATGATG ATGAGCTGAC TTTCGCTGAG GGTGAGGTCA TTGTGGTAAC TGGTGAGGAA
    GATCAGGAGT GGTGGATTGG GCACATCGAA GGACAGCCGG AGAGAAAAGG TGTATTTCCC
    ATGTCCTTTG TTCATATCTT ATCAGATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005162662.1
    CDS558..3827
    Translation

    Target ORF information:

    RefSeq Version XM_005162605.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 1b (asap1b), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005162605.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCCAGATC AAATATCGGT GTCGGAGTTT CTGTCGGAAA CAACAGAGGA TTACAACTCT 
    CCCACTACAT CCAGCTTCAC CACCCGCTTG CAGAGCTGCA GGAATACAGT CAGCGTGTTA
    GAAGAGGCTT TGGATCAGGA CAGAACGTCT TTGCAGAAGG TCAAGAAGTC AGTCAAAGCC
    ATTTACAACT CTGGCCAAGA TCACATTCAA AATGAGGAAA CGTATTCCCA GGCATTGGAT
    AAATTTGGCA GCAACTTCAT CAGTCGGGAT AATCCAGATC TGGGCACAGC CTTTGTTAAA
    TTCTCCACTT TGACTAAAGA GCTTTCTGCC CTCCTCAAGA ACCTGCTTCA GAATTTAAGT
    CATAATGTGA TCTTTACACT GGACTCCCTG TTGAAAGGCG ACCTGAAAGG CGTGAAGGGG
    GACCTGAAAA AACCCTTTGA CAAGGCTTGG AAGGACTACG AAACCAAGTT TACAAAAATC
    GAAAAGGAAA AACGTGAGCA TGCCAAACAG CATGGAATGA TCCGAACAGA GATCACTGGT
    GCTGAGATCG CAGAGGAGAT GGAGAAAGAA AGGAGGCTCT TCCAGCTACA GATGTGTGAG
    TACCTGATCA AAGTAAATGA AATAAAGACC AAGAAAGGAG TTGACCTCCT TCAGAACTTG
    ATCAAGTATT ACCACGCGCA ATGCAACTTT TTCCAAGATG GCTTGAAGAC GGCAGATAAA
    CTGAAGCAGT ACATTGAGAA GTTAGCAGCT GATCTTTACA ATATAAAACA AACACAAGAT
    GAAGAGAAAA AGCAGCTCAC GGCTTTAAGA GATCTCATTA AATCCTCTCT ACAAGTGGAC
    CAAAAGGAGT CTAGAAGACA GGACTCTCAG AGCAAACAGG GTGGCTACAG TATGCACCAG
    CTGCAGGGTA ACAAAGAGTT TGGCTGTGAG AAGAAAGGCT ATCTGTTAAA GAAGAGTGAT
    GGGTTAAGGA AGGTGTGGCA GAGAAGGAAG TGCTCAGTTA AAGGCGGGAT TCTCACCATC
    TCTCACGCCA CTTCAAACAG GCAGCCTGTC AAACTCAACC TGCTCACATG CCAGGTCAAG
    CCCAGTGCTG AGGACAGAAA GTGCTTCGAT CTTATTTCCC ATAACCGCAC ATATCACTTT
    CAGGCAGAAG ATGAGCAGGA ATTTATAATA TGGATCTCAG TGTTAACTAA CAGCAAGGAG
    GAGGCACTTA ACATGGCCTT CAGAGGAGAG CAGGGTGGAG GAGATGATGG GCTGGAGGAT
    CTCACCAAAG CCATAATCGA TGATGTGCTG CGCATGCCGG GAAATGAGTG GTGCTGTGAT
    TGTGGGGCTC CAGATCCCAA ATGGTTGTCG ACTAACCTGG GAATCCTGAC ATGCATCGAA
    TGTTCAGGCA TACACCGGGA GATGGGCGTC CACATCTCAC GCATCCAGTC TATGGAGCTA
    GACAAACTGG GAACCTCCGA ACTCTTGCTG GCCAAGAATG TGGGGAACAG TAGTTTTAAT
    GATATAATGG AGGGAGGTCT TTCTTGTCCC TCTTCTAAAC CCACTCCCTC TAGTGACATG
    ACTGTTCGTA AGGAGTTCAT CAATGCTAAA TATGTAGATC ATAAGTTTGC AAGGAAAACC
    TGCAGCTCAG CAGAAGACAA GAGGAGTGAT CTTTTTAAAG CCGTCGGGTC ACATGACCTG
    CTAGCTCTCA TCCAGGTGTA TGCTGAGGGG GTAGAGCTTT TGGAACCACT ACATGAAGGA
    GGACAGGAAG GTGGAGAGAC TGCATTGCAC TATGCCGTGA GGACAGCTGA CCAAACCTCA
    CTGCACCTGG TCGACTTCCT TGTCCAGAAC AGTGGGAATT TGGATAAGCA GACGGAGCGA
    GGAAACACGG CCCTGCACTA TTGCTGCCTG TACGAGAAAC ATGAATGCCT CAAACTACTG
    CTCCGAGGCA AACCAGCTAC TGATATCACC AATCAGAATG GGGACACAGC TTTGGATGTG
    GCTAGGCGGA TGAAGCTCTT CCAGTGTGAG GAAGCATTGG TACAAGCTGC TGCTGGAAAA
    TTTAATACTA AAGTCCATGT GGAATATGAA TGGAATCTCA GGTTGGAGGA GCTTGATGAA
    AGTGATGATG ATCTGGATTA CAAACCAAGT CCCATTAAGA AGGATCGCTC ACCCCGTCCG
    CAGAGTTTCT GCCACTCATC CAGCCTCTCC CCTCATGATA AGCTCTCTCT GCCTGCTTTC
    ATTGGTCAGC GTGATAAGCA ACGCCTCTCC TACTCTGCAT TCACCAATCA GATGTACAGC
    GTTTCCACCG ACTGTCCGTC CTCTCCGGTT TCAGATGCTC CACCTCTGCC GCCAAGGAAT
    GCTGGCAAAG CTCCATCTCT GGCTTTCCTT GGCTCTCATT CCCACTATGC TACTCTGGCT
    AGCACTCGAC CATACATCAG AAGTGATTCA ACGCCGCCTC CTGTCAACAA GATGTCCCCT
    GTAACTAATA GATTTGAGGG CATCCCACAA CAGCAAAGTC CCAACACCAC CAAGCCCACA
    CTTGGTCCAA GGGTCCTTCC CAAACTACCT CAGAAGGTTG CCCTGCGTAA GATAGACACC
    ATACATCACC CATCTGTGGA CAAAACCAAC CAGCCTTCTG AAGCTGTCCT TTTTCAGAAA
    GTTCTGCAGA GTTCAGATAC ATCCCAGAAA GTTCCGATAG CTGACAGGCC TCAGCCTGGA
    GACGTCGCTC CACAGCGACC ACAGCCTTCA GAATTACCAC CTAAACCAGG AGAACTGCCT
    CCAAAGCCTC AACTGTCCGA ACTTCCCCCT AAACCCCAGT TGGGTGACCT ACCACCTAAA
    CCTCAGCTTA AAGATCTCCC GCCCAAGCCC CAACTCGCAG ACATCTCCCC AGCCAAACCC
    ATAACCATTG AGGTCCCCCC AAAACCTTCT CCAGGAGAAG TGGCTCCCAA GCCCCAACCT
    CCAGATACTC CAACAATCAT TCAACAAGGT GAACTGTCAC CTCAGCCTGT GATCCAGTCT
    AGTGAAGATA CCAATGGAAG TCCAACATCT GCACAGGACA CACCCGTACC ATTGCCCAGA
    AAAATAAATC CGATTAAGAG TAAAATGCGA AGGGTGAAGA CTATTTATGA TTGCCAGGCA
    GACAATGATG ATGAGCTGAC TTTCGCTGAG GGTGAGGTCA TTGTGGTAAC TGGTGAGGAA
    GATCAGGAGT GGTGGATTGG GCACATCGAA GGACAGCCGG AGAGAAAAGG TGTATTTCCC
    ATGTCCTTTG TTCATATCTT ATCAGATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa28403
    Clone ID Related Accession (Same CDS sequence) XM_005162606.3
    Accession Version XM_005162606.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3201bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X8
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005162606.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGCCAGATC AAATATCGGT GTCGGAGTTT CTGTCGGAAA CAACAGAGGA TTACAACTCT 
    CCCACTACAT CCAGCTTCAC CACCCGCTTG CAGAGCTGCA GGAATACAGT CAGCGTGTTA
    GAAGAGGCTT TGGATCAGGA CAGAACGTCT TTGCAGAAGG TCAAGAAGTC AGTCAAAGCC
    ATTTACAACT CTGGCCAAGA TCACATTCAA AATGAGGAAA CGTATTCCCA GGCATTGGAT
    AAATTTGGCA GCAACTTCAT CAGTCGGGAT AATCCAGATC TGGGCACAGC CTTTGTTAAA
    TTCTCCACTT TGACTAAAGA GCTTTCTGCC CTCCTCAAGA ACCTGCTTCA GAATTTAAGT
    CATAATGTGA TCTTTACACT GGACTCCCTG TTGAAAGGCG ACCTGAAAGG CGTGAAGGGG
    GACCTGAAAA AACCCTTTGA CAAGGCTTGG AAGGACTACG AAACCAAGTT TACAAAAATC
    GAAAAGGAAA AACGTGAGCA TGCCAAACAG CATGGAATGA TCCGAACAGA GATCACTGGT
    GCTGAGATCG CAGAGGAGAT GGAGAAAGAA AGGAGGCTCT TCCAGCTACA GATGTGTGAG
    TACCTGATCA AAGTAAATGA AATAAAGACC AAGAAAGGAG TTGACCTCCT TCAGAACTTG
    ATCAAGTATT ACCACGCGCA ATGCAACTTT TTCCAAGATG GCTTGAAGAC GGCAGATAAA
    CTGAAGCAGT ACATTGAGAA GTTAGCAGCT GATCTTTACA ATATAAAACA AACACAAGAT
    GAAGAGAAAA AGCAGCTCAC GGCTTTAAGA GATCTCATTA AATCCTCTCT ACAAGTGGAC
    CAAAAGGAGT CTAGAAGACA GGACTCTCAG AGCAAACAGG GTGGCTACAG TATGCACCAG
    CTGCAGGGTA ACAAAGAGTT TGGCTGTGAG AAGAAAGGCT ATCTGTTAAA GAAGAGTGAT
    GGGTTAAGGA AGGTGTGGCA GAGAAGGAAG TGCTCAGTTA AAGGCGGGAT TCTCACCATC
    TCTCACGCCA CTTCAAACAG GCAGCCTGTC AAACTCAACC TGCTCACATG CCAGGTCAAG
    CCCAGTGCTG AGGACAGAAA GTGCTTCGAT CTTATTTCCC ATAACCGCAC ATATCACTTT
    CAGGCAGAAG ATGAGCAGGA ATTTATAATA TGGATCTCAG TGTTAACTAA CAGCAAGGAG
    GAGGCACTTA ACATGGCCTT CAGAGGAGAG CAGGGTGGAG GAGATGATGG GCTGGAGGAT
    CTCACCAAAG CCATAATCGA TGATGTGCTG CGCATGCCGG GAAATGAGTG GTGCTGTGAT
    TGTGGGGCTC CAGATCCCAA ATGGTTGTCG ACTAACCTGG GAATCCTGAC ATGCATCGAA
    TGTTCAGGCA TACACCGGGA GATGGGCGTC CACATCTCAC GCATCCAGTC TATGGAGCTA
    GACAAACTGG GAACCTCCGA ACTCTTGCTG GCCAAGAATG TGGGGAACAG TAGTTTTAAT
    GATATAATGG AGGGAGGTCT TTCTTGTCCC TCTTCTAAAC CCACTCCCTC TAGTGACATG
    ACTGTTCGTA AGGAGTTCAT CAATGCTAAA TATGTAGATC ATAAGTTTGC AAGGAAAACC
    TGCAGCTCAG CAGAAGACAA GAGGAGTGAT CTTTTTAAAG CCGTCGGGTC ACATGACCTG
    CTAGCTCTCA TCCAGGTGTA TGCTGAGGGG GTAGAGCTTT TGGAACCACT ACATGAAGGA
    GGACAGGAAG GTGGAGAGAC TGCATTGCAC TATGCCGTGA GGACAGCTGA CCAAACCTCA
    CTGCACCTGG TCGACTTCCT TGTCCAGAAC AGTGGGAATT TGGATAAGCA GACGGAGCGA
    GGAAACACGG CCCTGCACTA TTGCTGCCTG TACGAGAAAC ATGAATGCCT CAAACTACTG
    CTCCGAGGCA AACCAGCTAC TGATATCACC AATCAGAATG GGGACACAGC TTTGGATGTG
    GCTAGGCGGA TGAAGCTCTT CCAGTGTGAG GAAGCATTGG TACAAGCTGC TGCTGGAAAA
    TTTAATACTA AAGTCCATGT GGAATATGAA TGGAATCTCA GGTTGGAGGA GCTTGATGAA
    AGTGATGATG ATCTGGATTA CAAACCAAGT CCCATTAAGA AGGATCGCTC ACCCCGTCCG
    CAGAGTTTCT GCCACTCATC CAGCCTCTCC CCTCATGATA AGCTCTCTCT GCCTGCTTTC
    ATTGGTCAGC GTGATAAGCA ACGCCTCTCC TACTCTGCAT TCACCAATCA GATGTACAGC
    GTTTCCACCG ACTGTCCGTC CTCTCCGGTT TCAGATGCTC CACCTCTGCC GCCAAGGAAT
    GCTGGCAAAG GAAGTGATTC AACGCCGCCT CCTGTCAACA AGATGTCCCC TGTAACTAAT
    AGATTTGAGG GCATCCCACA ACAGCAAAGT CCCAACACCA CCAAGCCCAC ACTTGGTCCA
    AGGGTCCTTC CCAAACTACC TCAGAAGGTT GCCCTGCGTA AGATAGACAC CATACATCAC
    CCATCTGTGG ACAAAACCAA CCAGCCTTCT GAAGCTGTCC TTTTTCAGAA AGTTCTGCAG
    AGTTCAGATA CATCCCAGAA AGTTCCGATA GCTGACAGGC CTCAGCCTGG AGACGTCGCT
    CCACAGCGAC CACAGCCTTC AGAATTACCA CCTAAACCAG GAGAACTGCC TCCAAAGCCT
    CAACTGTCCG AACTTCCCCC TAAACCCCAG TTGGGTGACC TACCACCTAA ACCTCAGCTT
    AAAGATCTCC CGCCCAAGCC CCAACTCGCA GACATCTCCC CAGCCAAACC CATAACCATT
    GAGGTCCCCC CAAAACCTTC TCCAGGAGAA GTGGCTCCCA AGCCCCAACC TCCAGATACT
    CCAACAATCA TTCAACAAGG TGAACTGTCA CCTCAGCCTG TGATCCAGTC TAGTGAAGAT
    ACCAATGGAA GTCCAACATC TGCACAGGAC ACACCCGTAC CATTGCCCAG AAAAATAAAT
    CCGATTAAGA GTAAAATGCG AAGGGTGAAG ACTATTTATG ATTGCCAGGC AGACAATGAT
    GATGAGCTGA CTTTCGCTGA GGGTGAGGTC ATTGTGGTAA CTGGTGAGGA AGATCAGGAG
    TGGTGGATTG GGCACATCGA AGGACAGCCG GAGAGAAAAG GTGTATTTCC CATGTCCTTT
    GTTCATATCT TATCAGATTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005162663.1
    CDS558..3758
    Translation

    Target ORF information:

    RefSeq Version XM_005162606.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 1b (asap1b), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005162606.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGCCAGATC AAATATCGGT GTCGGAGTTT CTGTCGGAAA CAACAGAGGA TTACAACTCT 
    CCCACTACAT CCAGCTTCAC CACCCGCTTG CAGAGCTGCA GGAATACAGT CAGCGTGTTA
    GAAGAGGCTT TGGATCAGGA CAGAACGTCT TTGCAGAAGG TCAAGAAGTC AGTCAAAGCC
    ATTTACAACT CTGGCCAAGA TCACATTCAA AATGAGGAAA CGTATTCCCA GGCATTGGAT
    AAATTTGGCA GCAACTTCAT CAGTCGGGAT AATCCAGATC TGGGCACAGC CTTTGTTAAA
    TTCTCCACTT TGACTAAAGA GCTTTCTGCC CTCCTCAAGA ACCTGCTTCA GAATTTAAGT
    CATAATGTGA TCTTTACACT GGACTCCCTG TTGAAAGGCG ACCTGAAAGG CGTGAAGGGG
    GACCTGAAAA AACCCTTTGA CAAGGCTTGG AAGGACTACG AAACCAAGTT TACAAAAATC
    GAAAAGGAAA AACGTGAGCA TGCCAAACAG CATGGAATGA TCCGAACAGA GATCACTGGT
    GCTGAGATCG CAGAGGAGAT GGAGAAAGAA AGGAGGCTCT TCCAGCTACA GATGTGTGAG
    TACCTGATCA AAGTAAATGA AATAAAGACC AAGAAAGGAG TTGACCTCCT TCAGAACTTG
    ATCAAGTATT ACCACGCGCA ATGCAACTTT TTCCAAGATG GCTTGAAGAC GGCAGATAAA
    CTGAAGCAGT ACATTGAGAA GTTAGCAGCT GATCTTTACA ATATAAAACA AACACAAGAT
    GAAGAGAAAA AGCAGCTCAC GGCTTTAAGA GATCTCATTA AATCCTCTCT ACAAGTGGAC
    CAAAAGGAGT CTAGAAGACA GGACTCTCAG AGCAAACAGG GTGGCTACAG TATGCACCAG
    CTGCAGGGTA ACAAAGAGTT TGGCTGTGAG AAGAAAGGCT ATCTGTTAAA GAAGAGTGAT
    GGGTTAAGGA AGGTGTGGCA GAGAAGGAAG TGCTCAGTTA AAGGCGGGAT TCTCACCATC
    TCTCACGCCA CTTCAAACAG GCAGCCTGTC AAACTCAACC TGCTCACATG CCAGGTCAAG
    CCCAGTGCTG AGGACAGAAA GTGCTTCGAT CTTATTTCCC ATAACCGCAC ATATCACTTT
    CAGGCAGAAG ATGAGCAGGA ATTTATAATA TGGATCTCAG TGTTAACTAA CAGCAAGGAG
    GAGGCACTTA ACATGGCCTT CAGAGGAGAG CAGGGTGGAG GAGATGATGG GCTGGAGGAT
    CTCACCAAAG CCATAATCGA TGATGTGCTG CGCATGCCGG GAAATGAGTG GTGCTGTGAT
    TGTGGGGCTC CAGATCCCAA ATGGTTGTCG ACTAACCTGG GAATCCTGAC ATGCATCGAA
    TGTTCAGGCA TACACCGGGA GATGGGCGTC CACATCTCAC GCATCCAGTC TATGGAGCTA
    GACAAACTGG GAACCTCCGA ACTCTTGCTG GCCAAGAATG TGGGGAACAG TAGTTTTAAT
    GATATAATGG AGGGAGGTCT TTCTTGTCCC TCTTCTAAAC CCACTCCCTC TAGTGACATG
    ACTGTTCGTA AGGAGTTCAT CAATGCTAAA TATGTAGATC ATAAGTTTGC AAGGAAAACC
    TGCAGCTCAG CAGAAGACAA GAGGAGTGAT CTTTTTAAAG CCGTCGGGTC ACATGACCTG
    CTAGCTCTCA TCCAGGTGTA TGCTGAGGGG GTAGAGCTTT TGGAACCACT ACATGAAGGA
    GGACAGGAAG GTGGAGAGAC TGCATTGCAC TATGCCGTGA GGACAGCTGA CCAAACCTCA
    CTGCACCTGG TCGACTTCCT TGTCCAGAAC AGTGGGAATT TGGATAAGCA GACGGAGCGA
    GGAAACACGG CCCTGCACTA TTGCTGCCTG TACGAGAAAC ATGAATGCCT CAAACTACTG
    CTCCGAGGCA AACCAGCTAC TGATATCACC AATCAGAATG GGGACACAGC TTTGGATGTG
    GCTAGGCGGA TGAAGCTCTT CCAGTGTGAG GAAGCATTGG TACAAGCTGC TGCTGGAAAA
    TTTAATACTA AAGTCCATGT GGAATATGAA TGGAATCTCA GGTTGGAGGA GCTTGATGAA
    AGTGATGATG ATCTGGATTA CAAACCAAGT CCCATTAAGA AGGATCGCTC ACCCCGTCCG
    CAGAGTTTCT GCCACTCATC CAGCCTCTCC CCTCATGATA AGCTCTCTCT GCCTGCTTTC
    ATTGGTCAGC GTGATAAGCA ACGCCTCTCC TACTCTGCAT TCACCAATCA GATGTACAGC
    GTTTCCACCG ACTGTCCGTC CTCTCCGGTT TCAGATGCTC CACCTCTGCC GCCAAGGAAT
    GCTGGCAAAG GAAGTGATTC AACGCCGCCT CCTGTCAACA AGATGTCCCC TGTAACTAAT
    AGATTTGAGG GCATCCCACA ACAGCAAAGT CCCAACACCA CCAAGCCCAC ACTTGGTCCA
    AGGGTCCTTC CCAAACTACC TCAGAAGGTT GCCCTGCGTA AGATAGACAC CATACATCAC
    CCATCTGTGG ACAAAACCAA CCAGCCTTCT GAAGCTGTCC TTTTTCAGAA AGTTCTGCAG
    AGTTCAGATA CATCCCAGAA AGTTCCGATA GCTGACAGGC CTCAGCCTGG AGACGTCGCT
    CCACAGCGAC CACAGCCTTC AGAATTACCA CCTAAACCAG GAGAACTGCC TCCAAAGCCT
    CAACTGTCCG AACTTCCCCC TAAACCCCAG TTGGGTGACC TACCACCTAA ACCTCAGCTT
    AAAGATCTCC CGCCCAAGCC CCAACTCGCA GACATCTCCC CAGCCAAACC CATAACCATT
    GAGGTCCCCC CAAAACCTTC TCCAGGAGAA GTGGCTCCCA AGCCCCAACC TCCAGATACT
    CCAACAATCA TTCAACAAGG TGAACTGTCA CCTCAGCCTG TGATCCAGTC TAGTGAAGAT
    ACCAATGGAA GTCCAACATC TGCACAGGAC ACACCCGTAC CATTGCCCAG AAAAATAAAT
    CCGATTAAGA GTAAAATGCG AAGGGTGAAG ACTATTTATG ATTGCCAGGC AGACAATGAT
    GATGAGCTGA CTTTCGCTGA GGGTGAGGTC ATTGTGGTAA CTGGTGAGGA AGATCAGGAG
    TGGTGGATTG GGCACATCGA AGGACAGCCG GAGAGAAAAG GTGTATTTCC CATGTCCTTT
    GTTCATATCT TATCAGATTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa28404
    Clone ID Related Accession (Same CDS sequence) XM_003201277.4
    Accession Version XM_003201277.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3189bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X9
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_003201277.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGCCAGATC AAATATCGGT GTCGGAGTTT CTGTCGGAAA CAACAGAGGA TTACAACTCT 
    CCCACTACAT CCAGCTTCAC CACCCGCTTG CAGAGCTGCA GGAATACAGT CAGCGTGTTA
    GAAGAGGCTT TGGATCAGGA CAGAACGTCT TTGCAGAAGG TCAAGAAGTC AGTCAAAGCC
    ATTTACAACT CTGGCCAAGA TCACATTCAA AATGAGGAAA CGTATTCCCA GGCATTGGAT
    AAATTTGGCA GCAACTTCAT CAGTCGGGAT AATCCAGATC TGGGCACAGC CTTTGTTAAA
    TTCTCCACTT TGACTAAAGA GCTTTCTGCC CTCCTCAAGA ACCTGCTTCA GAATTTAAGT
    CATAATGTGA TCTTTACACT GGACTCCCTG TTGAAAGGCG ACCTGAAAGG CGTGAAGGGG
    GACCTGAAAA AACCCTTTGA CAAGGCTTGG AAGGACTACG AAACCAAGTT TACAAAAATC
    GAAAAGGAAA AACGTGAGCA TGCCAAACAG CATGGAATGA TCCGAACAGA GATCACTGGT
    GCTGAGATCG CAGAGGAGAT GGAGAAAGAA AGGAGGCTCT TCCAGCTACA GATGTGTGAG
    TACCTGATCA AAGTAAATGA AATAAAGACC AAGAAAGGAG TTGACCTCCT TCAGAACTTG
    ATCAAGTATT ACCACGCGCA ATGCAACTTT TTCCAAGATG GCTTGAAGAC GGCAGATAAA
    CTGAAGCAGT ACATTGAGAA GTTAGCAGCT GATCTTTACA ATATAAAACA AACACAAGAT
    GAAGAGAAAA AGCAGCTCAC GGCTTTAAGA GATCTCATTA AATCCTCTCT ACAAGTGGAC
    CAAAAGGAGG ACTCTCAGAG CAAACAGGGT GGCTACAGTA TGCACCAGCT GCAGGGTAAC
    AAAGAGTTTG GCTGTGAGAA GAAAGGCTAT CTGTTAAAGA AGAGTGATGG GTTAAGGAAG
    GTGTGGCAGA GAAGGAAGTG CTCAGTTAAA GGCGGGATTC TCACCATCTC TCACGCCACT
    TCAAACAGGC AGCCTGTCAA ACTCAACCTG CTCACATGCC AGGTCAAGCC CAGTGCTGAG
    GACAGAAAGT GCTTCGATCT TATTTCCCAT AACCGCACAT ATCACTTTCA GGCAGAAGAT
    GAGCAGGAAT TTATAATATG GATCTCAGTG TTAACTAACA GCAAGGAGGA GGCACTTAAC
    ATGGCCTTCA GAGGAGAGCA GGGTGGAGGA GATGATGGGC TGGAGGATCT CACCAAAGCC
    ATAATCGATG ATGTGCTGCG CATGCCGGGA AATGAGTGGT GCTGTGATTG TGGGGCTCCA
    GATCCCAAAT GGTTGTCGAC TAACCTGGGA ATCCTGACAT GCATCGAATG TTCAGGCATA
    CACCGGGAGA TGGGCGTCCA CATCTCACGC ATCCAGTCTA TGGAGCTAGA CAAACTGGGA
    ACCTCCGAAC TCTTGCTGGC CAAGAATGTG GGGAACAGTA GTTTTAATGA TATAATGGAG
    GGAGGTCTTT CTTGTCCCTC TTCTAAACCC ACTCCCTCTA GTGACATGAC TGTTCGTAAG
    GAGTTCATCA ATGCTAAATA TGTAGATCAT AAGTTTGCAA GGAAAACCTG CAGCTCAGCA
    GAAGACAAGA GGAGTGATCT TTTTAAAGCC GTCGGGTCAC ATGACCTGCT AGCTCTCATC
    CAGGTGTATG CTGAGGGGGT AGAGCTTTTG GAACCACTAC ATGAAGGAGG ACAGGAAGGT
    GGAGAGACTG CATTGCACTA TGCCGTGAGG ACAGCTGACC AAACCTCACT GCACCTGGTC
    GACTTCCTTG TCCAGAACAG TGGGAATTTG GATAAGCAGA CGGAGCGAGG AAACACGGCC
    CTGCACTATT GCTGCCTGTA CGAGAAACAT GAATGCCTCA AACTACTGCT CCGAGGCAAA
    CCAGCTACTG ATATCACCAA TCAGAATGGG GACACAGCTT TGGATGTGGC TAGGCGGATG
    AAGCTCTTCC AGTGTGAGGA AGCATTGGTA CAAGCTGCTG CTGGAAAATT TAATACTAAA
    GTCCATGTGG AATATGAATG GAATCTCAGG TTGGAGGAGC TTGATGAAAG TGATGATGAT
    CTGGATTACA AACCAAGTCC CATTAAGAAG GATCGCTCAC CCCGTCCGCA GAGTTTCTGC
    CACTCATCCA GCCTCTCCCC TCATGATAAG CTCTCTCTGC CTGCTTTCAT TGGTCAGCGT
    GATAAGCAAC GCCTCTCCTA CTCTGCATTC ACCAATCAGA TGTACAGCGT TTCCACCGAC
    TGTCCGTCCT CTCCGGTTTC AGATGCTCCA CCTCTGCCGC CAAGGAATGC TGGCAAAGGA
    AGTGATTCAA CGCCGCCTCC TGTCAACAAG ATGTCCCCTG TAACTAATAG ATTTGAGGGC
    ATCCCACAAC AGCAAAGTCC CAACACCACC AAGCCCACAC TTGGTCCAAG GGTCCTTCCC
    AAACTACCTC AGAAGGTTGC CCTGCGTAAG ATAGACACCA TACATCACCC ATCTGTGGAC
    AAAACCAACC AGCCTTCTGA AGCTGTCCTT TTTCAGAAAG TTCTGCAGAG TTCAGATACA
    TCCCAGAAAG TTCCGATAGC TGACAGGCCT CAGCCTGGAG ACGTCGCTCC ACAGCGACCA
    CAGCCTTCAG AATTACCACC TAAACCAGGA GAACTGCCTC CAAAGCCTCA ACTGTCCGAA
    CTTCCCCCTA AACCCCAGTT GGGTGACCTA CCACCTAAAC CTCAGCTTAA AGATCTCCCG
    CCCAAGCCCC AACTCGCAGA CATCTCCCCA GCCAAACCCA TAACCATTGA GGTCCCCCCA
    AAACCTTCTC CAGGAGAAGT GGCTCCCAAG CCCCAACCTC CAGATACTCC AACAATCATT
    CAACAAGGTG AACTGTCACC TCAGCCTGTG ATCCAGTCTA GTGAAGATAC CAATGGAAGT
    CCAACATCTG CACAGGACAC ACCCGTACCA TTGCCCAGAA AAATAAATCC GATTAAGAGT
    AAAATGCGAA GGGTGAAGAC TATTTATGAT TGCCAGGCAG ACAATGATGA TGAGCTGACT
    TTCGCTGAGG GTGAGGTCAT TGTGGTAACT GGTGAGGAAG ATCAGGAGTG GTGGATTGGG
    CACATCGAAG GACAGCCGGA GAGAAAAGGT GTATTTCCCA TGTCCTTTGT TCATATCTTA
    TCAGATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003201325.1
    CDS558..3746
    Translation

    Target ORF information:

    RefSeq Version XM_003201277.4
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 1b (asap1b), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003201277.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGCCAGATC AAATATCGGT GTCGGAGTTT CTGTCGGAAA CAACAGAGGA TTACAACTCT 
    CCCACTACAT CCAGCTTCAC CACCCGCTTG CAGAGCTGCA GGAATACAGT CAGCGTGTTA
    GAAGAGGCTT TGGATCAGGA CAGAACGTCT TTGCAGAAGG TCAAGAAGTC AGTCAAAGCC
    ATTTACAACT CTGGCCAAGA TCACATTCAA AATGAGGAAA CGTATTCCCA GGCATTGGAT
    AAATTTGGCA GCAACTTCAT CAGTCGGGAT AATCCAGATC TGGGCACAGC CTTTGTTAAA
    TTCTCCACTT TGACTAAAGA GCTTTCTGCC CTCCTCAAGA ACCTGCTTCA GAATTTAAGT
    CATAATGTGA TCTTTACACT GGACTCCCTG TTGAAAGGCG ACCTGAAAGG CGTGAAGGGG
    GACCTGAAAA AACCCTTTGA CAAGGCTTGG AAGGACTACG AAACCAAGTT TACAAAAATC
    GAAAAGGAAA AACGTGAGCA TGCCAAACAG CATGGAATGA TCCGAACAGA GATCACTGGT
    GCTGAGATCG CAGAGGAGAT GGAGAAAGAA AGGAGGCTCT TCCAGCTACA GATGTGTGAG
    TACCTGATCA AAGTAAATGA AATAAAGACC AAGAAAGGAG TTGACCTCCT TCAGAACTTG
    ATCAAGTATT ACCACGCGCA ATGCAACTTT TTCCAAGATG GCTTGAAGAC GGCAGATAAA
    CTGAAGCAGT ACATTGAGAA GTTAGCAGCT GATCTTTACA ATATAAAACA AACACAAGAT
    GAAGAGAAAA AGCAGCTCAC GGCTTTAAGA GATCTCATTA AATCCTCTCT ACAAGTGGAC
    CAAAAGGAGG ACTCTCAGAG CAAACAGGGT GGCTACAGTA TGCACCAGCT GCAGGGTAAC
    AAAGAGTTTG GCTGTGAGAA GAAAGGCTAT CTGTTAAAGA AGAGTGATGG GTTAAGGAAG
    GTGTGGCAGA GAAGGAAGTG CTCAGTTAAA GGCGGGATTC TCACCATCTC TCACGCCACT
    TCAAACAGGC AGCCTGTCAA ACTCAACCTG CTCACATGCC AGGTCAAGCC CAGTGCTGAG
    GACAGAAAGT GCTTCGATCT TATTTCCCAT AACCGCACAT ATCACTTTCA GGCAGAAGAT
    GAGCAGGAAT TTATAATATG GATCTCAGTG TTAACTAACA GCAAGGAGGA GGCACTTAAC
    ATGGCCTTCA GAGGAGAGCA GGGTGGAGGA GATGATGGGC TGGAGGATCT CACCAAAGCC
    ATAATCGATG ATGTGCTGCG CATGCCGGGA AATGAGTGGT GCTGTGATTG TGGGGCTCCA
    GATCCCAAAT GGTTGTCGAC TAACCTGGGA ATCCTGACAT GCATCGAATG TTCAGGCATA
    CACCGGGAGA TGGGCGTCCA CATCTCACGC ATCCAGTCTA TGGAGCTAGA CAAACTGGGA
    ACCTCCGAAC TCTTGCTGGC CAAGAATGTG GGGAACAGTA GTTTTAATGA TATAATGGAG
    GGAGGTCTTT CTTGTCCCTC TTCTAAACCC ACTCCCTCTA GTGACATGAC TGTTCGTAAG
    GAGTTCATCA ATGCTAAATA TGTAGATCAT AAGTTTGCAA GGAAAACCTG CAGCTCAGCA
    GAAGACAAGA GGAGTGATCT TTTTAAAGCC GTCGGGTCAC ATGACCTGCT AGCTCTCATC
    CAGGTGTATG CTGAGGGGGT AGAGCTTTTG GAACCACTAC ATGAAGGAGG ACAGGAAGGT
    GGAGAGACTG CATTGCACTA TGCCGTGAGG ACAGCTGACC AAACCTCACT GCACCTGGTC
    GACTTCCTTG TCCAGAACAG TGGGAATTTG GATAAGCAGA CGGAGCGAGG AAACACGGCC
    CTGCACTATT GCTGCCTGTA CGAGAAACAT GAATGCCTCA AACTACTGCT CCGAGGCAAA
    CCAGCTACTG ATATCACCAA TCAGAATGGG GACACAGCTT TGGATGTGGC TAGGCGGATG
    AAGCTCTTCC AGTGTGAGGA AGCATTGGTA CAAGCTGCTG CTGGAAAATT TAATACTAAA
    GTCCATGTGG AATATGAATG GAATCTCAGG TTGGAGGAGC TTGATGAAAG TGATGATGAT
    CTGGATTACA AACCAAGTCC CATTAAGAAG GATCGCTCAC CCCGTCCGCA GAGTTTCTGC
    CACTCATCCA GCCTCTCCCC TCATGATAAG CTCTCTCTGC CTGCTTTCAT TGGTCAGCGT
    GATAAGCAAC GCCTCTCCTA CTCTGCATTC ACCAATCAGA TGTACAGCGT TTCCACCGAC
    TGTCCGTCCT CTCCGGTTTC AGATGCTCCA CCTCTGCCGC CAAGGAATGC TGGCAAAGGA
    AGTGATTCAA CGCCGCCTCC TGTCAACAAG ATGTCCCCTG TAACTAATAG ATTTGAGGGC
    ATCCCACAAC AGCAAAGTCC CAACACCACC AAGCCCACAC TTGGTCCAAG GGTCCTTCCC
    AAACTACCTC AGAAGGTTGC CCTGCGTAAG ATAGACACCA TACATCACCC ATCTGTGGAC
    AAAACCAACC AGCCTTCTGA AGCTGTCCTT TTTCAGAAAG TTCTGCAGAG TTCAGATACA
    TCCCAGAAAG TTCCGATAGC TGACAGGCCT CAGCCTGGAG ACGTCGCTCC ACAGCGACCA
    CAGCCTTCAG AATTACCACC TAAACCAGGA GAACTGCCTC CAAAGCCTCA ACTGTCCGAA
    CTTCCCCCTA AACCCCAGTT GGGTGACCTA CCACCTAAAC CTCAGCTTAA AGATCTCCCG
    CCCAAGCCCC AACTCGCAGA CATCTCCCCA GCCAAACCCA TAACCATTGA GGTCCCCCCA
    AAACCTTCTC CAGGAGAAGT GGCTCCCAAG CCCCAACCTC CAGATACTCC AACAATCATT
    CAACAAGGTG AACTGTCACC TCAGCCTGTG ATCCAGTCTA GTGAAGATAC CAATGGAAGT
    CCAACATCTG CACAGGACAC ACCCGTACCA TTGCCCAGAA AAATAAATCC GATTAAGAGT
    AAAATGCGAA GGGTGAAGAC TATTTATGAT TGCCAGGCAG ACAATGATGA TGAGCTGACT
    TTCGCTGAGG GTGAGGTCAT TGTGGTAACT GGTGAGGAAG ATCAGGAGTG GTGGATTGGG
    CACATCGAAG GACAGCCGGA GAGAAAAGGT GTATTTCCCA TGTCCTTTGT TCATATCTTA
    TCAGATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44731
    Clone ID Related Accession (Same CDS sequence) XM_009297309.2
    Accession Version XM_009297309.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3354bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009297309.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCCAGATC AAATATCGGT GTCGGAGTTT CTGTCGGAAA CAACAGAGGA TTACAACTCT 
    CCCACTACAT CCAGCTTCAC CACCCGCTTG CAGAGCTGCA GGAATACAGT CAGCGTGTTA
    GAAGAGGCTT TGGATCAGGA CAGAACGTCT TTGCAGAAGG TCAAGAAGTC AGTCAAAGCC
    ATTTACAACT CTGGCCAAGA TCACATTCAA AATGAGGAAA CGTATTCCCA GGCATTGGAT
    AAATTTGGCA GCAACTTCAT CAGTCGGGAT AATCCAGATC TGGGCACAGC CTTTGTTAAA
    TTCTCCACTT TGACTAAAGA GCTTTCTGCC CTCCTCAAGA ACCTGCTTCA GAATTTAAGT
    CATAATGTGA TCTTTACACT GGACTCCCTG TTGAAAGGCG ACCTGAAAGG CGTGAAGGGG
    GACCTGAAAA AACCCTTTGA CAAGGCTTGG AAGGACTACG AAACCAAGTT TACAAAAATC
    GAAAAGGAAA AACGTGAGCA TGCCAAACAG CATGGAATGA TCCGAACAGA GATCACTGGT
    GCTGAGATCG CAGAGGAGAT GGAGAAAGAA AGGAGGCTCT TCCAGCTACA GATGTGTGAG
    TACCTGATCA AAGTAAATGA AATAAAGACC AAGAAAGGAG TTGACCTCCT TCAGAACTTG
    ATCAAGTATT ACCACGCGCA ATGCAACTTT TTCCAAGATG GCTTGAAGAC GGCAGATAAA
    CTGAAGCAGT ACATTGAGAA GTTAGCAGCT GATCTTTACA ATATAAAACA AACACAAGAT
    GAAGAGAAAA AGCAGCTCAC GGCTTTAAGA GATCTCATTA AATCCTCTCT ACAAGTGGAC
    CAAAAGGAGT CTAGAAGACA GGACTCTCAG AGCAAACAGG GTGGCTACAG TATGCACCAG
    CTGCAGGGTA ACAAAGAGTT TGGCTGTGAG AAGAAAGGCT ATCTGTTAAA GAAGAGTGAT
    GGGTTAAGGA AGGTGTGGCA GAGAAGGAAG TGCTCAGTTA AAGGCGGGAT TCTCACCATC
    TCTCACGCCA CTTCAAACAG GCAGCCTGTC AAACTCAACC TGCTCACATG CCAGGTCAAG
    CCCAGTGCTG AGGACAGAAA GTGCTTCGAT CTTATTTCCC ATAACCGCAC ATATCACTTT
    CAGGCAGAAG ATGAGCAGGA ATTTATAATA TGGATCTCAG TGTTAACTAA CAGCAAGGAG
    GAGGCACTTA ACATGGCCTT CAGAGGAGAG CAGGGTGGAG GAGATGATGG GCTGGAGGAT
    CTCACCAAAG CCATAATCGA TGATGTGCTG CGCATGCCGG GAAATGAGTG GTGCTGTGAT
    TGTGGGGCTC CAGATCCCAA ATGGTTGTCG ACTAACCTGG GAATCCTGAC ATGCATCGAA
    TGTTCAGGCA TACACCGGGA GATGGGCGTC CACATCTCAC GCATCCAGTC TATGGAGCTA
    GACAAACTGG GAACCTCCGA ACTCTTGCTG GCCAAGAATG TGGGGAACAG TAGTTTTAAT
    GATATAATGG AGGGAGGTCT TTCTTGTCCC TCTTCTAAAC CCACTCCCTC TAGTGACATG
    ACTGTTCGTA AGGAGTTCAT CAATGCTAAA TATGTAGATC ATAAGTTTGC AAGGAAAACC
    TGCAGCTCAG CAGAAGACAA GAGGAGTGAT CTTTTTAAAG CCGTCGGGTC ACATGACCTG
    CTAGCTCTCA TCCAGGTGTA TGCTGAGGGG GTAGAGCTTT TGGAACCACT ACATGAAGGA
    GGACAGGAAG GTGGAGAGAC TGCATTGCAC TATGCCGTGA GGACAGCTGA CCAAACCTCA
    CTGCACCTGG TCGACTTCCT TGTCCAGAAC AGTGGGAATT TGGATAAGCA GACGGAGCGA
    GGAAACACGG CCCTGCACTA TTGCTGCCTG TACGAGAAAC ATGAATGCCT CAAACTACTG
    CTCCGAGGCA AACCAGCTAC TGATATCACC AATCAGAATG GGGACACAGC TTTGGATGTG
    GCTAGGCGGA TGAAGCTCTT CCAGTGTGAG GAAGCATTGG TACAAGCTGC TGCTGGAAAA
    TTTAATACTA AAGTCCATGT GGAATATGAA TGGAATCTCA GGTTGGAGGA GCTTGATGAA
    AGTGATGATG ATCTGGATTA CAAACCAAGT CCCATTAAGA AGGATCGCTC ACCCCGTCCG
    CAGAGTTTCT GCCACTCATC CAGCCTCTCC CCTCATGATA AGCTCTCTCT GCCTGCTTTC
    ATTGGTCAGC GTGATAAGCA ACGCCTCTCC TACTCTGCAT TCACCAATCA GATGTACAGC
    GTTTCCACCG ACTGTCCGTC CTCTCCGGTT TCAGATGCTC CACCTCTGCC GCCAAGGAAT
    GCTGGCAAAG CCCCTCCATT GTCGAACCTG CCTCCCTCCA CCCTTGCCCC TGCATCACAG
    ACCTCACCTA GTGGCAGCTC TACCCTGTCA AAGAAGAGAC CCCCTCCCCC TCCCCCTGGA
    CACAAACGCA CGCTATCTGA CCCCCCTACC CCTCTCTTTA AAGGAAGTGA TTCAACGCCG
    CCTCCTGTCA ACAAGATGTC CCCTGTAACT AATAGATTTG AGGGCATCCC ACAACAGCAA
    AGTCCCAACA CCACCAAGCC CACACTTGGT CCAAGGGTCC TTCCCAAACT ACCTCAGAAG
    GTTGCCCTGC GTAAGATAGA CACCATACAT CACCCATCTG TGGACAAAAC CAACCAGCCT
    TCTGAAGCTG TCCTTTTTCA GAAAGTTCTG CAGAGTTCAG ATACATCCCA GAAAGTTCCG
    ATAGCTGACA GGCCTCAGCC TGGAGACGTC GCTCCACAGC GACCACAGCC TTCAGAATTA
    CCACCTAAAC CAGGAGAACT GCCTCCAAAG CCTCAACTGT CCGAACTTCC CCCTAAACCC
    CAGTTGGGTG ACCTACCACC TAAACCTCAG CTTAAAGATC TCCCGCCCAA GCCCCAACTC
    GCAGACATCT CCCCAGCCAA ACCCATAACC ATTGAGGTCC CCCCAAAACC TTCTCCAGGA
    GAAGTGGCTC CCAAGCCCCA ACCTCCAGAT ACTCCAACAA TCATTCAACA AGGTGAACTG
    TCACCTCAGC CTGTGATCCA GTCTAGTGAA GATACCAATG GAAGTCCAAC ATCTGCACAG
    GACACACCCG TACCATTGCC CAGAAAAATA AATCCGATTA AGAGTAAAAT GCGAAGGGTG
    AAGACTATTT ATGATTGCCA GGCAGACAAT GATGATGAGC TGACTTTCGC TGAGGGTGAG
    GTCATTGTGG TAACTGGTGA GGAAGATCAG GAGTGGTGGA TTGGGCACAT CGAAGGACAG
    CCGGAGAGAA AAGGTGTATT TCCCATGTCC TTTGTTCATA TCTTATCAGA TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295584.1
    CDS558..3911
    Translation

    Target ORF information:

    RefSeq Version XM_009297309.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 1b (asap1b), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009297309.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCCAGATC AAATATCGGT GTCGGAGTTT CTGTCGGAAA CAACAGAGGA TTACAACTCT 
    CCCACTACAT CCAGCTTCAC CACCCGCTTG CAGAGCTGCA GGAATACAGT CAGCGTGTTA
    GAAGAGGCTT TGGATCAGGA CAGAACGTCT TTGCAGAAGG TCAAGAAGTC AGTCAAAGCC
    ATTTACAACT CTGGCCAAGA TCACATTCAA AATGAGGAAA CGTATTCCCA GGCATTGGAT
    AAATTTGGCA GCAACTTCAT CAGTCGGGAT AATCCAGATC TGGGCACAGC CTTTGTTAAA
    TTCTCCACTT TGACTAAAGA GCTTTCTGCC CTCCTCAAGA ACCTGCTTCA GAATTTAAGT
    CATAATGTGA TCTTTACACT GGACTCCCTG TTGAAAGGCG ACCTGAAAGG CGTGAAGGGG
    GACCTGAAAA AACCCTTTGA CAAGGCTTGG AAGGACTACG AAACCAAGTT TACAAAAATC
    GAAAAGGAAA AACGTGAGCA TGCCAAACAG CATGGAATGA TCCGAACAGA GATCACTGGT
    GCTGAGATCG CAGAGGAGAT GGAGAAAGAA AGGAGGCTCT TCCAGCTACA GATGTGTGAG
    TACCTGATCA AAGTAAATGA AATAAAGACC AAGAAAGGAG TTGACCTCCT TCAGAACTTG
    ATCAAGTATT ACCACGCGCA ATGCAACTTT TTCCAAGATG GCTTGAAGAC GGCAGATAAA
    CTGAAGCAGT ACATTGAGAA GTTAGCAGCT GATCTTTACA ATATAAAACA AACACAAGAT
    GAAGAGAAAA AGCAGCTCAC GGCTTTAAGA GATCTCATTA AATCCTCTCT ACAAGTGGAC
    CAAAAGGAGT CTAGAAGACA GGACTCTCAG AGCAAACAGG GTGGCTACAG TATGCACCAG
    CTGCAGGGTA ACAAAGAGTT TGGCTGTGAG AAGAAAGGCT ATCTGTTAAA GAAGAGTGAT
    GGGTTAAGGA AGGTGTGGCA GAGAAGGAAG TGCTCAGTTA AAGGCGGGAT TCTCACCATC
    TCTCACGCCA CTTCAAACAG GCAGCCTGTC AAACTCAACC TGCTCACATG CCAGGTCAAG
    CCCAGTGCTG AGGACAGAAA GTGCTTCGAT CTTATTTCCC ATAACCGCAC ATATCACTTT
    CAGGCAGAAG ATGAGCAGGA ATTTATAATA TGGATCTCAG TGTTAACTAA CAGCAAGGAG
    GAGGCACTTA ACATGGCCTT CAGAGGAGAG CAGGGTGGAG GAGATGATGG GCTGGAGGAT
    CTCACCAAAG CCATAATCGA TGATGTGCTG CGCATGCCGG GAAATGAGTG GTGCTGTGAT
    TGTGGGGCTC CAGATCCCAA ATGGTTGTCG ACTAACCTGG GAATCCTGAC ATGCATCGAA
    TGTTCAGGCA TACACCGGGA GATGGGCGTC CACATCTCAC GCATCCAGTC TATGGAGCTA
    GACAAACTGG GAACCTCCGA ACTCTTGCTG GCCAAGAATG TGGGGAACAG TAGTTTTAAT
    GATATAATGG AGGGAGGTCT TTCTTGTCCC TCTTCTAAAC CCACTCCCTC TAGTGACATG
    ACTGTTCGTA AGGAGTTCAT CAATGCTAAA TATGTAGATC ATAAGTTTGC AAGGAAAACC
    TGCAGCTCAG CAGAAGACAA GAGGAGTGAT CTTTTTAAAG CCGTCGGGTC ACATGACCTG
    CTAGCTCTCA TCCAGGTGTA TGCTGAGGGG GTAGAGCTTT TGGAACCACT ACATGAAGGA
    GGACAGGAAG GTGGAGAGAC TGCATTGCAC TATGCCGTGA GGACAGCTGA CCAAACCTCA
    CTGCACCTGG TCGACTTCCT TGTCCAGAAC AGTGGGAATT TGGATAAGCA GACGGAGCGA
    GGAAACACGG CCCTGCACTA TTGCTGCCTG TACGAGAAAC ATGAATGCCT CAAACTACTG
    CTCCGAGGCA AACCAGCTAC TGATATCACC AATCAGAATG GGGACACAGC TTTGGATGTG
    GCTAGGCGGA TGAAGCTCTT CCAGTGTGAG GAAGCATTGG TACAAGCTGC TGCTGGAAAA
    TTTAATACTA AAGTCCATGT GGAATATGAA TGGAATCTCA GGTTGGAGGA GCTTGATGAA
    AGTGATGATG ATCTGGATTA CAAACCAAGT CCCATTAAGA AGGATCGCTC ACCCCGTCCG
    CAGAGTTTCT GCCACTCATC CAGCCTCTCC CCTCATGATA AGCTCTCTCT GCCTGCTTTC
    ATTGGTCAGC GTGATAAGCA ACGCCTCTCC TACTCTGCAT TCACCAATCA GATGTACAGC
    GTTTCCACCG ACTGTCCGTC CTCTCCGGTT TCAGATGCTC CACCTCTGCC GCCAAGGAAT
    GCTGGCAAAG CCCCTCCATT GTCGAACCTG CCTCCCTCCA CCCTTGCCCC TGCATCACAG
    ACCTCACCTA GTGGCAGCTC TACCCTGTCA AAGAAGAGAC CCCCTCCCCC TCCCCCTGGA
    CACAAACGCA CGCTATCTGA CCCCCCTACC CCTCTCTTTA AAGGAAGTGA TTCAACGCCG
    CCTCCTGTCA ACAAGATGTC CCCTGTAACT AATAGATTTG AGGGCATCCC ACAACAGCAA
    AGTCCCAACA CCACCAAGCC CACACTTGGT CCAAGGGTCC TTCCCAAACT ACCTCAGAAG
    GTTGCCCTGC GTAAGATAGA CACCATACAT CACCCATCTG TGGACAAAAC CAACCAGCCT
    TCTGAAGCTG TCCTTTTTCA GAAAGTTCTG CAGAGTTCAG ATACATCCCA GAAAGTTCCG
    ATAGCTGACA GGCCTCAGCC TGGAGACGTC GCTCCACAGC GACCACAGCC TTCAGAATTA
    CCACCTAAAC CAGGAGAACT GCCTCCAAAG CCTCAACTGT CCGAACTTCC CCCTAAACCC
    CAGTTGGGTG ACCTACCACC TAAACCTCAG CTTAAAGATC TCCCGCCCAA GCCCCAACTC
    GCAGACATCT CCCCAGCCAA ACCCATAACC ATTGAGGTCC CCCCAAAACC TTCTCCAGGA
    GAAGTGGCTC CCAAGCCCCA ACCTCCAGAT ACTCCAACAA TCATTCAACA AGGTGAACTG
    TCACCTCAGC CTGTGATCCA GTCTAGTGAA GATACCAATG GAAGTCCAAC ATCTGCACAG
    GACACACCCG TACCATTGCC CAGAAAAATA AATCCGATTA AGAGTAAAAT GCGAAGGGTG
    AAGACTATTT ATGATTGCCA GGCAGACAAT GATGATGAGC TGACTTTCGC TGAGGGTGAG
    GTCATTGTGG TAACTGGTGA GGAAGATCAG GAGTGGTGGA TTGGGCACAT CGAAGGACAG
    CCGGAGAGAA AAGGTGTATT TCCCATGTCC TTTGTTCATA TCTTATCAGA TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44732
    Clone ID Related Accession (Same CDS sequence) XM_009297310.2
    Accession Version XM_009297310.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3351bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009297310.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCCAGATC AAATATCGGT GTCGGAGTTT CTGTCGGAAA CAACAGAGGA TTACAACTCT 
    CCCACTACAT CCAGCTTCAC CACCCGCTTG CAGAGCTGCA GGAATACAGT CAGCGTGTTA
    GAAGAGGCTT TGGATCAGGA CAGAACGTCT TTGCAGAAGG TCAAGAAGTC AGTCAAAGCC
    ATTTACAACT CTGGCCAAGA TCACATTCAA AATGAGGAAA CGTATTCCCA GGCATTGGAT
    AAATTTGGCA GCAACTTCAT CAGTCGGGAT AATCCAGATC TGGGCACAGC CTTTGTTAAA
    TTCTCCACTT TGACTAAAGA GCTTTCTGCC CTCCTCAAGA ACCTGCTTCA GAATTTAAGT
    CATAATGTGA TCTTTACACT GGACTCCCTG TTGAAAGGCG ACCTGAAAGG CGTGAAGGGG
    GACCTGAAAA AACCCTTTGA CAAGGCTTGG AAGGACTACG AAACCAAGTT TACAAAAATC
    GAAAAGGAAA AACGTGAGCA TGCCAAACAG CATGGAATGA TCCGAACAGA GATCACTGGT
    GCTGAGATCG CAGAGGAGAT GGAGAAAGAA AGGAGGCTCT TCCAGCTACA GATGTGTGAG
    TACCTGATCA AAGTAAATGA AATAAAGACC AAGAAAGGAG TTGACCTCCT TCAGAACTTG
    ATCAAGTATT ACCACGCGCA ATGCAACTTT TTCCAAGATG GCTTGAAGAC GGCAGATAAA
    CTGAAGCAGT ACATTGAGAA GTTAGCAGCT GATCTTTACA ATATAAAACA AACACAAGAT
    GAAGAGAAAA AGCAGCTCAC GGCTTTAAGA GATCTCATTA AATCCTCTCT ACAAGTGGAC
    CAAAAGGAGT CTAGAAGAGA CTCTCAGAGC AAACAGGGTG GCTACAGTAT GCACCAGCTG
    CAGGGTAACA AAGAGTTTGG CTGTGAGAAG AAAGGCTATC TGTTAAAGAA GAGTGATGGG
    TTAAGGAAGG TGTGGCAGAG AAGGAAGTGC TCAGTTAAAG GCGGGATTCT CACCATCTCT
    CACGCCACTT CAAACAGGCA GCCTGTCAAA CTCAACCTGC TCACATGCCA GGTCAAGCCC
    AGTGCTGAGG ACAGAAAGTG CTTCGATCTT ATTTCCCATA ACCGCACATA TCACTTTCAG
    GCAGAAGATG AGCAGGAATT TATAATATGG ATCTCAGTGT TAACTAACAG CAAGGAGGAG
    GCACTTAACA TGGCCTTCAG AGGAGAGCAG GGTGGAGGAG ATGATGGGCT GGAGGATCTC
    ACCAAAGCCA TAATCGATGA TGTGCTGCGC ATGCCGGGAA ATGAGTGGTG CTGTGATTGT
    GGGGCTCCAG ATCCCAAATG GTTGTCGACT AACCTGGGAA TCCTGACATG CATCGAATGT
    TCAGGCATAC ACCGGGAGAT GGGCGTCCAC ATCTCACGCA TCCAGTCTAT GGAGCTAGAC
    AAACTGGGAA CCTCCGAACT CTTGCTGGCC AAGAATGTGG GGAACAGTAG TTTTAATGAT
    ATAATGGAGG GAGGTCTTTC TTGTCCCTCT TCTAAACCCA CTCCCTCTAG TGACATGACT
    GTTCGTAAGG AGTTCATCAA TGCTAAATAT GTAGATCATA AGTTTGCAAG GAAAACCTGC
    AGCTCAGCAG AAGACAAGAG GAGTGATCTT TTTAAAGCCG TCGGGTCACA TGACCTGCTA
    GCTCTCATCC AGGTGTATGC TGAGGGGGTA GAGCTTTTGG AACCACTACA TGAAGGAGGA
    CAGGAAGGTG GAGAGACTGC ATTGCACTAT GCCGTGAGGA CAGCTGACCA AACCTCACTG
    CACCTGGTCG ACTTCCTTGT CCAGAACAGT GGGAATTTGG ATAAGCAGAC GGAGCGAGGA
    AACACGGCCC TGCACTATTG CTGCCTGTAC GAGAAACATG AATGCCTCAA ACTACTGCTC
    CGAGGCAAAC CAGCTACTGA TATCACCAAT CAGAATGGGG ACACAGCTTT GGATGTGGCT
    AGGCGGATGA AGCTCTTCCA GTGTGAGGAA GCATTGGTAC AAGCTGCTGC TGGAAAATTT
    AATACTAAAG TCCATGTGGA ATATGAATGG AATCTCAGGT TGGAGGAGCT TGATGAAAGT
    GATGATGATC TGGATTACAA ACCAAGTCCC ATTAAGAAGG ATCGCTCACC CCGTCCGCAG
    AGTTTCTGCC ACTCATCCAG CCTCTCCCCT CATGATAAGC TCTCTCTGCC TGCTTTCATT
    GGTCAGCGTG ATAAGCAACG CCTCTCCTAC TCTGCATTCA CCAATCAGAT GTACAGCGTT
    TCCACCGACT GTCCGTCCTC TCCGGTTTCA GATGCTCCAC CTCTGCCGCC AAGGAATGCT
    GGCAAAGCCC CTCCATTGTC GAACCTGCCT CCCTCCACCC TTGCCCCTGC ATCACAGACC
    TCACCTAGTG GCAGCTCTAC CCTGTCAAAG AAGAGACCCC CTCCCCCTCC CCCTGGACAC
    AAACGCACGC TATCTGACCC CCCTACCCCT CTCTTTAAAG GAAGTGATTC AACGCCGCCT
    CCTGTCAACA AGATGTCCCC TGTAACTAAT AGATTTGAGG GCATCCCACA ACAGCAAAGT
    CCCAACACCA CCAAGCCCAC ACTTGGTCCA AGGGTCCTTC CCAAACTACC TCAGAAGGTT
    GCCCTGCGTA AGATAGACAC CATACATCAC CCATCTGTGG ACAAAACCAA CCAGCCTTCT
    GAAGCTGTCC TTTTTCAGAA AGTTCTGCAG AGTTCAGATA CATCCCAGAA AGTTCCGATA
    GCTGACAGGC CTCAGCCTGG AGACGTCGCT CCACAGCGAC CACAGCCTTC AGAATTACCA
    CCTAAACCAG GAGAACTGCC TCCAAAGCCT CAACTGTCCG AACTTCCCCC TAAACCCCAG
    TTGGGTGACC TACCACCTAA ACCTCAGCTT AAAGATCTCC CGCCCAAGCC CCAACTCGCA
    GACATCTCCC CAGCCAAACC CATAACCATT GAGGTCCCCC CAAAACCTTC TCCAGGAGAA
    GTGGCTCCCA AGCCCCAACC TCCAGATACT CCAACAATCA TTCAACAAGG TGAACTGTCA
    CCTCAGCCTG TGATCCAGTC TAGTGAAGAT ACCAATGGAA GTCCAACATC TGCACAGGAC
    ACACCCGTAC CATTGCCCAG AAAAATAAAT CCGATTAAGA GTAAAATGCG AAGGGTGAAG
    ACTATTTATG ATTGCCAGGC AGACAATGAT GATGAGCTGA CTTTCGCTGA GGGTGAGGTC
    ATTGTGGTAA CTGGTGAGGA AGATCAGGAG TGGTGGATTG GGCACATCGA AGGACAGCCG
    GAGAGAAAAG GTGTATTTCC CATGTCCTTT GTTCATATCT TATCAGATTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295585.1
    CDS558..3908
    Translation

    Target ORF information:

    RefSeq Version XM_009297310.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 1b (asap1b), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009297310.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCCAGATC AAATATCGGT GTCGGAGTTT CTGTCGGAAA CAACAGAGGA TTACAACTCT 
    CCCACTACAT CCAGCTTCAC CACCCGCTTG CAGAGCTGCA GGAATACAGT CAGCGTGTTA
    GAAGAGGCTT TGGATCAGGA CAGAACGTCT TTGCAGAAGG TCAAGAAGTC AGTCAAAGCC
    ATTTACAACT CTGGCCAAGA TCACATTCAA AATGAGGAAA CGTATTCCCA GGCATTGGAT
    AAATTTGGCA GCAACTTCAT CAGTCGGGAT AATCCAGATC TGGGCACAGC CTTTGTTAAA
    TTCTCCACTT TGACTAAAGA GCTTTCTGCC CTCCTCAAGA ACCTGCTTCA GAATTTAAGT
    CATAATGTGA TCTTTACACT GGACTCCCTG TTGAAAGGCG ACCTGAAAGG CGTGAAGGGG
    GACCTGAAAA AACCCTTTGA CAAGGCTTGG AAGGACTACG AAACCAAGTT TACAAAAATC
    GAAAAGGAAA AACGTGAGCA TGCCAAACAG CATGGAATGA TCCGAACAGA GATCACTGGT
    GCTGAGATCG CAGAGGAGAT GGAGAAAGAA AGGAGGCTCT TCCAGCTACA GATGTGTGAG
    TACCTGATCA AAGTAAATGA AATAAAGACC AAGAAAGGAG TTGACCTCCT TCAGAACTTG
    ATCAAGTATT ACCACGCGCA ATGCAACTTT TTCCAAGATG GCTTGAAGAC GGCAGATAAA
    CTGAAGCAGT ACATTGAGAA GTTAGCAGCT GATCTTTACA ATATAAAACA AACACAAGAT
    GAAGAGAAAA AGCAGCTCAC GGCTTTAAGA GATCTCATTA AATCCTCTCT ACAAGTGGAC
    CAAAAGGAGT CTAGAAGAGA CTCTCAGAGC AAACAGGGTG GCTACAGTAT GCACCAGCTG
    CAGGGTAACA AAGAGTTTGG CTGTGAGAAG AAAGGCTATC TGTTAAAGAA GAGTGATGGG
    TTAAGGAAGG TGTGGCAGAG AAGGAAGTGC TCAGTTAAAG GCGGGATTCT CACCATCTCT
    CACGCCACTT CAAACAGGCA GCCTGTCAAA CTCAACCTGC TCACATGCCA GGTCAAGCCC
    AGTGCTGAGG ACAGAAAGTG CTTCGATCTT ATTTCCCATA ACCGCACATA TCACTTTCAG
    GCAGAAGATG AGCAGGAATT TATAATATGG ATCTCAGTGT TAACTAACAG CAAGGAGGAG
    GCACTTAACA TGGCCTTCAG AGGAGAGCAG GGTGGAGGAG ATGATGGGCT GGAGGATCTC
    ACCAAAGCCA TAATCGATGA TGTGCTGCGC ATGCCGGGAA ATGAGTGGTG CTGTGATTGT
    GGGGCTCCAG ATCCCAAATG GTTGTCGACT AACCTGGGAA TCCTGACATG CATCGAATGT
    TCAGGCATAC ACCGGGAGAT GGGCGTCCAC ATCTCACGCA TCCAGTCTAT GGAGCTAGAC
    AAACTGGGAA CCTCCGAACT CTTGCTGGCC AAGAATGTGG GGAACAGTAG TTTTAATGAT
    ATAATGGAGG GAGGTCTTTC TTGTCCCTCT TCTAAACCCA CTCCCTCTAG TGACATGACT
    GTTCGTAAGG AGTTCATCAA TGCTAAATAT GTAGATCATA AGTTTGCAAG GAAAACCTGC
    AGCTCAGCAG AAGACAAGAG GAGTGATCTT TTTAAAGCCG TCGGGTCACA TGACCTGCTA
    GCTCTCATCC AGGTGTATGC TGAGGGGGTA GAGCTTTTGG AACCACTACA TGAAGGAGGA
    CAGGAAGGTG GAGAGACTGC ATTGCACTAT GCCGTGAGGA CAGCTGACCA AACCTCACTG
    CACCTGGTCG ACTTCCTTGT CCAGAACAGT GGGAATTTGG ATAAGCAGAC GGAGCGAGGA
    AACACGGCCC TGCACTATTG CTGCCTGTAC GAGAAACATG AATGCCTCAA ACTACTGCTC
    CGAGGCAAAC CAGCTACTGA TATCACCAAT CAGAATGGGG ACACAGCTTT GGATGTGGCT
    AGGCGGATGA AGCTCTTCCA GTGTGAGGAA GCATTGGTAC AAGCTGCTGC TGGAAAATTT
    AATACTAAAG TCCATGTGGA ATATGAATGG AATCTCAGGT TGGAGGAGCT TGATGAAAGT
    GATGATGATC TGGATTACAA ACCAAGTCCC ATTAAGAAGG ATCGCTCACC CCGTCCGCAG
    AGTTTCTGCC ACTCATCCAG CCTCTCCCCT CATGATAAGC TCTCTCTGCC TGCTTTCATT
    GGTCAGCGTG ATAAGCAACG CCTCTCCTAC TCTGCATTCA CCAATCAGAT GTACAGCGTT
    TCCACCGACT GTCCGTCCTC TCCGGTTTCA GATGCTCCAC CTCTGCCGCC AAGGAATGCT
    GGCAAAGCCC CTCCATTGTC GAACCTGCCT CCCTCCACCC TTGCCCCTGC ATCACAGACC
    TCACCTAGTG GCAGCTCTAC CCTGTCAAAG AAGAGACCCC CTCCCCCTCC CCCTGGACAC
    AAACGCACGC TATCTGACCC CCCTACCCCT CTCTTTAAAG GAAGTGATTC AACGCCGCCT
    CCTGTCAACA AGATGTCCCC TGTAACTAAT AGATTTGAGG GCATCCCACA ACAGCAAAGT
    CCCAACACCA CCAAGCCCAC ACTTGGTCCA AGGGTCCTTC CCAAACTACC TCAGAAGGTT
    GCCCTGCGTA AGATAGACAC CATACATCAC CCATCTGTGG ACAAAACCAA CCAGCCTTCT
    GAAGCTGTCC TTTTTCAGAA AGTTCTGCAG AGTTCAGATA CATCCCAGAA AGTTCCGATA
    GCTGACAGGC CTCAGCCTGG AGACGTCGCT CCACAGCGAC CACAGCCTTC AGAATTACCA
    CCTAAACCAG GAGAACTGCC TCCAAAGCCT CAACTGTCCG AACTTCCCCC TAAACCCCAG
    TTGGGTGACC TACCACCTAA ACCTCAGCTT AAAGATCTCC CGCCCAAGCC CCAACTCGCA
    GACATCTCCC CAGCCAAACC CATAACCATT GAGGTCCCCC CAAAACCTTC TCCAGGAGAA
    GTGGCTCCCA AGCCCCAACC TCCAGATACT CCAACAATCA TTCAACAAGG TGAACTGTCA
    CCTCAGCCTG TGATCCAGTC TAGTGAAGAT ACCAATGGAA GTCCAACATC TGCACAGGAC
    ACACCCGTAC CATTGCCCAG AAAAATAAAT CCGATTAAGA GTAAAATGCG AAGGGTGAAG
    ACTATTTATG ATTGCCAGGC AGACAATGAT GATGAGCTGA CTTTCGCTGA GGGTGAGGTC
    ATTGTGGTAA CTGGTGAGGA AGATCAGGAG TGGTGGATTG GGCACATCGA AGGACAGCCG
    GAGAGAAAAG GTGTATTTCC CATGTCCTTT GTTCATATCT TATCAGATTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44734
    Clone ID Related Accession (Same CDS sequence) XM_009297312.2
    Accession Version XM_009297312.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3342bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009297312.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCCAGATC AAATATCGGT GTCGGAGTTT CTGTCGGAAA CAACAGAGGA TTACAACTCT 
    CCCACTACAT CCAGCTTCAC CACCCGCTTG CAGAGCTGCA GGAATACAGT CAGCGTGTTA
    GAAGAGGCTT TGGATCAGGA CAGAACGTCT TTGCAGAAGG TCAAGAAGTC AGTCAAAGCC
    ATTTACAACT CTGGCCAAGA TCACATTCAA AATGAGGAAA CGTATTCCCA GGCATTGGAT
    AAATTTGGCA GCAACTTCAT CAGTCGGGAT AATCCAGATC TGGGCACAGC CTTTGTTAAA
    TTCTCCACTT TGACTAAAGA GCTTTCTGCC CTCCTCAAGA ACCTGCTTCA GAATTTAAGT
    CATAATGTGA TCTTTACACT GGACTCCCTG TTGAAAGGCG ACCTGAAAGG CGTGAAGGGG
    GACCTGAAAA AACCCTTTGA CAAGGCTTGG AAGGACTACG AAACCAAGTT TACAAAAATC
    GAAAAGGAAA AACGTGAGCA TGCCAAACAG CATGGAATGA TCCGAACAGA GATCACTGGT
    GCTGAGATCG CAGAGGAGAT GGAGAAAGAA AGGAGGCTCT TCCAGCTACA GATGTGTGAG
    TACCTGATCA AAGTAAATGA AATAAAGACC AAGAAAGGAG TTGACCTCCT TCAGAACTTG
    ATCAAGTATT ACCACGCGCA ATGCAACTTT TTCCAAGATG GCTTGAAGAC GGCAGATAAA
    CTGAAGCAGT ACATTGAGAA GTTAGCAGCT GATCTTTACA ATATAAAACA AACACAAGAT
    GAAGAGAAAA AGCAGCTCAC GGCTTTAAGA GATCTCATTA AATCCTCTCT ACAAGTGGAC
    CAAAAGGAGG ACTCTCAGAG CAAACAGGGT GGCTACAGTA TGCACCAGCT GCAGGGTAAC
    AAAGAGTTTG GCTGTGAGAA GAAAGGCTAT CTGTTAAAGA AGAGTGATGG GTTAAGGAAG
    GTGTGGCAGA GAAGGAAGTG CTCAGTTAAA GGCGGGATTC TCACCATCTC TCACGCCACT
    TCAAACAGGC AGCCTGTCAA ACTCAACCTG CTCACATGCC AGGTCAAGCC CAGTGCTGAG
    GACAGAAAGT GCTTCGATCT TATTTCCCAT AACCGCACAT ATCACTTTCA GGCAGAAGAT
    GAGCAGGAAT TTATAATATG GATCTCAGTG TTAACTAACA GCAAGGAGGA GGCACTTAAC
    ATGGCCTTCA GAGGAGAGCA GGGTGGAGGA GATGATGGGC TGGAGGATCT CACCAAAGCC
    ATAATCGATG ATGTGCTGCG CATGCCGGGA AATGAGTGGT GCTGTGATTG TGGGGCTCCA
    GATCCCAAAT GGTTGTCGAC TAACCTGGGA ATCCTGACAT GCATCGAATG TTCAGGCATA
    CACCGGGAGA TGGGCGTCCA CATCTCACGC ATCCAGTCTA TGGAGCTAGA CAAACTGGGA
    ACCTCCGAAC TCTTGCTGGC CAAGAATGTG GGGAACAGTA GTTTTAATGA TATAATGGAG
    GGAGGTCTTT CTTGTCCCTC TTCTAAACCC ACTCCCTCTA GTGACATGAC TGTTCGTAAG
    GAGTTCATCA ATGCTAAATA TGTAGATCAT AAGTTTGCAA GGAAAACCTG CAGCTCAGCA
    GAAGACAAGA GGAGTGATCT TTTTAAAGCC GTCGGGTCAC ATGACCTGCT AGCTCTCATC
    CAGGTGTATG CTGAGGGGGT AGAGCTTTTG GAACCACTAC ATGAAGGAGG ACAGGAAGGT
    GGAGAGACTG CATTGCACTA TGCCGTGAGG ACAGCTGACC AAACCTCACT GCACCTGGTC
    GACTTCCTTG TCCAGAACAG TGGGAATTTG GATAAGCAGA CGGAGCGAGG AAACACGGCC
    CTGCACTATT GCTGCCTGTA CGAGAAACAT GAATGCCTCA AACTACTGCT CCGAGGCAAA
    CCAGCTACTG ATATCACCAA TCAGAATGGG GACACAGCTT TGGATGTGGC TAGGCGGATG
    AAGCTCTTCC AGTGTGAGGA AGCATTGGTA CAAGCTGCTG CTGGAAAATT TAATACTAAA
    GTCCATGTGG AATATGAATG GAATCTCAGG TTGGAGGAGC TTGATGAAAG TGATGATGAT
    CTGGATTACA AACCAAGTCC CATTAAGAAG GATCGCTCAC CCCGTCCGCA GAGTTTCTGC
    CACTCATCCA GCCTCTCCCC TCATGATAAG CTCTCTCTGC CTGCTTTCAT TGGTCAGCGT
    GATAAGCAAC GCCTCTCCTA CTCTGCATTC ACCAATCAGA TGTACAGCGT TTCCACCGAC
    TGTCCGTCCT CTCCGGTTTC AGATGCTCCA CCTCTGCCGC CAAGGAATGC TGGCAAAGCC
    CCTCCATTGT CGAACCTGCC TCCCTCCACC CTTGCCCCTG CATCACAGAC CTCACCTAGT
    GGCAGCTCTA CCCTGTCAAA GAAGAGACCC CCTCCCCCTC CCCCTGGACA CAAACGCACG
    CTATCTGACC CCCCTACCCC TCTCTTTAAA GGAAGTGATT CAACGCCGCC TCCTGTCAAC
    AAGATGTCCC CTGTAACTAA TAGATTTGAG GGCATCCCAC AACAGCAAAG TCCCAACACC
    ACCAAGCCCA CACTTGGTCC AAGGGTCCTT CCCAAACTAC CTCAGAAGGT TGCCCTGCGT
    AAGATAGACA CCATACATCA CCCATCTGTG GACAAAACCA ACCAGCCTTC TGAAGCTGTC
    CTTTTTCAGA AAGTTCTGCA GAGTTCAGAT ACATCCCAGA AAGTTCCGAT AGCTGACAGG
    CCTCAGCCTG GAGACGTCGC TCCACAGCGA CCACAGCCTT CAGAATTACC ACCTAAACCA
    GGAGAACTGC CTCCAAAGCC TCAACTGTCC GAACTTCCCC CTAAACCCCA GTTGGGTGAC
    CTACCACCTA AACCTCAGCT TAAAGATCTC CCGCCCAAGC CCCAACTCGC AGACATCTCC
    CCAGCCAAAC CCATAACCAT TGAGGTCCCC CCAAAACCTT CTCCAGGAGA AGTGGCTCCC
    AAGCCCCAAC CTCCAGATAC TCCAACAATC ATTCAACAAG GTGAACTGTC ACCTCAGCCT
    GTGATCCAGT CTAGTGAAGA TACCAATGGA AGTCCAACAT CTGCACAGGA CACACCCGTA
    CCATTGCCCA GAAAAATAAA TCCGATTAAG AGTAAAATGC GAAGGGTGAA GACTATTTAT
    GATTGCCAGG CAGACAATGA TGATGAGCTG ACTTTCGCTG AGGGTGAGGT CATTGTGGTA
    ACTGGTGAGG AAGATCAGGA GTGGTGGATT GGGCACATCG AAGGACAGCC GGAGAGAAAA
    GGTGTATTTC CCATGTCCTT TGTTCATATC TTATCAGATT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295587.1
    CDS558..3899
    Translation

    Target ORF information:

    RefSeq Version XM_009297312.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 1b (asap1b), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009297312.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCCAGATC AAATATCGGT GTCGGAGTTT CTGTCGGAAA CAACAGAGGA TTACAACTCT 
    CCCACTACAT CCAGCTTCAC CACCCGCTTG CAGAGCTGCA GGAATACAGT CAGCGTGTTA
    GAAGAGGCTT TGGATCAGGA CAGAACGTCT TTGCAGAAGG TCAAGAAGTC AGTCAAAGCC
    ATTTACAACT CTGGCCAAGA TCACATTCAA AATGAGGAAA CGTATTCCCA GGCATTGGAT
    AAATTTGGCA GCAACTTCAT CAGTCGGGAT AATCCAGATC TGGGCACAGC CTTTGTTAAA
    TTCTCCACTT TGACTAAAGA GCTTTCTGCC CTCCTCAAGA ACCTGCTTCA GAATTTAAGT
    CATAATGTGA TCTTTACACT GGACTCCCTG TTGAAAGGCG ACCTGAAAGG CGTGAAGGGG
    GACCTGAAAA AACCCTTTGA CAAGGCTTGG AAGGACTACG AAACCAAGTT TACAAAAATC
    GAAAAGGAAA AACGTGAGCA TGCCAAACAG CATGGAATGA TCCGAACAGA GATCACTGGT
    GCTGAGATCG CAGAGGAGAT GGAGAAAGAA AGGAGGCTCT TCCAGCTACA GATGTGTGAG
    TACCTGATCA AAGTAAATGA AATAAAGACC AAGAAAGGAG TTGACCTCCT TCAGAACTTG
    ATCAAGTATT ACCACGCGCA ATGCAACTTT TTCCAAGATG GCTTGAAGAC GGCAGATAAA
    CTGAAGCAGT ACATTGAGAA GTTAGCAGCT GATCTTTACA ATATAAAACA AACACAAGAT
    GAAGAGAAAA AGCAGCTCAC GGCTTTAAGA GATCTCATTA AATCCTCTCT ACAAGTGGAC
    CAAAAGGAGG ACTCTCAGAG CAAACAGGGT GGCTACAGTA TGCACCAGCT GCAGGGTAAC
    AAAGAGTTTG GCTGTGAGAA GAAAGGCTAT CTGTTAAAGA AGAGTGATGG GTTAAGGAAG
    GTGTGGCAGA GAAGGAAGTG CTCAGTTAAA GGCGGGATTC TCACCATCTC TCACGCCACT
    TCAAACAGGC AGCCTGTCAA ACTCAACCTG CTCACATGCC AGGTCAAGCC CAGTGCTGAG
    GACAGAAAGT GCTTCGATCT TATTTCCCAT AACCGCACAT ATCACTTTCA GGCAGAAGAT
    GAGCAGGAAT TTATAATATG GATCTCAGTG TTAACTAACA GCAAGGAGGA GGCACTTAAC
    ATGGCCTTCA GAGGAGAGCA GGGTGGAGGA GATGATGGGC TGGAGGATCT CACCAAAGCC
    ATAATCGATG ATGTGCTGCG CATGCCGGGA AATGAGTGGT GCTGTGATTG TGGGGCTCCA
    GATCCCAAAT GGTTGTCGAC TAACCTGGGA ATCCTGACAT GCATCGAATG TTCAGGCATA
    CACCGGGAGA TGGGCGTCCA CATCTCACGC ATCCAGTCTA TGGAGCTAGA CAAACTGGGA
    ACCTCCGAAC TCTTGCTGGC CAAGAATGTG GGGAACAGTA GTTTTAATGA TATAATGGAG
    GGAGGTCTTT CTTGTCCCTC TTCTAAACCC ACTCCCTCTA GTGACATGAC TGTTCGTAAG
    GAGTTCATCA ATGCTAAATA TGTAGATCAT AAGTTTGCAA GGAAAACCTG CAGCTCAGCA
    GAAGACAAGA GGAGTGATCT TTTTAAAGCC GTCGGGTCAC ATGACCTGCT AGCTCTCATC
    CAGGTGTATG CTGAGGGGGT AGAGCTTTTG GAACCACTAC ATGAAGGAGG ACAGGAAGGT
    GGAGAGACTG CATTGCACTA TGCCGTGAGG ACAGCTGACC AAACCTCACT GCACCTGGTC
    GACTTCCTTG TCCAGAACAG TGGGAATTTG GATAAGCAGA CGGAGCGAGG AAACACGGCC
    CTGCACTATT GCTGCCTGTA CGAGAAACAT GAATGCCTCA AACTACTGCT CCGAGGCAAA
    CCAGCTACTG ATATCACCAA TCAGAATGGG GACACAGCTT TGGATGTGGC TAGGCGGATG
    AAGCTCTTCC AGTGTGAGGA AGCATTGGTA CAAGCTGCTG CTGGAAAATT TAATACTAAA
    GTCCATGTGG AATATGAATG GAATCTCAGG TTGGAGGAGC TTGATGAAAG TGATGATGAT
    CTGGATTACA AACCAAGTCC CATTAAGAAG GATCGCTCAC CCCGTCCGCA GAGTTTCTGC
    CACTCATCCA GCCTCTCCCC TCATGATAAG CTCTCTCTGC CTGCTTTCAT TGGTCAGCGT
    GATAAGCAAC GCCTCTCCTA CTCTGCATTC ACCAATCAGA TGTACAGCGT TTCCACCGAC
    TGTCCGTCCT CTCCGGTTTC AGATGCTCCA CCTCTGCCGC CAAGGAATGC TGGCAAAGCC
    CCTCCATTGT CGAACCTGCC TCCCTCCACC CTTGCCCCTG CATCACAGAC CTCACCTAGT
    GGCAGCTCTA CCCTGTCAAA GAAGAGACCC CCTCCCCCTC CCCCTGGACA CAAACGCACG
    CTATCTGACC CCCCTACCCC TCTCTTTAAA GGAAGTGATT CAACGCCGCC TCCTGTCAAC
    AAGATGTCCC CTGTAACTAA TAGATTTGAG GGCATCCCAC AACAGCAAAG TCCCAACACC
    ACCAAGCCCA CACTTGGTCC AAGGGTCCTT CCCAAACTAC CTCAGAAGGT TGCCCTGCGT
    AAGATAGACA CCATACATCA CCCATCTGTG GACAAAACCA ACCAGCCTTC TGAAGCTGTC
    CTTTTTCAGA AAGTTCTGCA GAGTTCAGAT ACATCCCAGA AAGTTCCGAT AGCTGACAGG
    CCTCAGCCTG GAGACGTCGC TCCACAGCGA CCACAGCCTT CAGAATTACC ACCTAAACCA
    GGAGAACTGC CTCCAAAGCC TCAACTGTCC GAACTTCCCC CTAAACCCCA GTTGGGTGAC
    CTACCACCTA AACCTCAGCT TAAAGATCTC CCGCCCAAGC CCCAACTCGC AGACATCTCC
    CCAGCCAAAC CCATAACCAT TGAGGTCCCC CCAAAACCTT CTCCAGGAGA AGTGGCTCCC
    AAGCCCCAAC CTCCAGATAC TCCAACAATC ATTCAACAAG GTGAACTGTC ACCTCAGCCT
    GTGATCCAGT CTAGTGAAGA TACCAATGGA AGTCCAACAT CTGCACAGGA CACACCCGTA
    CCATTGCCCA GAAAAATAAA TCCGATTAAG AGTAAAATGC GAAGGGTGAA GACTATTTAT
    GATTGCCAGG CAGACAATGA TGATGAGCTG ACTTTCGCTG AGGGTGAGGT CATTGTGGTA
    ACTGGTGAGG AAGATCAGGA GTGGTGGATT GGGCACATCG AAGGACAGCC GGAGAGAAAA
    GGTGTATTTC CCATGTCCTT TGTTCATATC TTATCAGATT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.