Asap1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Asap1
Entrez Gene ID	13196
Full Name	ArfGAP with SH3 domain, ankyrin repeat and PH domain1
Synonyms	AV239055,DEF-1,Ddef1,PAP,mKIAA1249,s19
General protein information	Preferred Names ArfGAP with SH3 domain, ankyrin repeat and PH domain1 Names arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 130 kDa phosphatidylinositol 4,5-biphosphate-dependent ARF1 GTPase-activating protein 130 kDa phosphatidylinositol 4,5-bisphosphate-dependent ARF1 GTPase-activating protein ADP-ribosylation factor-directed GTPase-activating protein 1 ARF GTPase-activating protein 1 PIP2-dependent ARF1 GAP development and differentiation-enhancing factor 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 15 Map Location: 15\|15 D1

mRNA and Protein(s)

mRNA	Protein	Name
XM_006520396.3	XP_006520459.1	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X5
XM_006520398.3	XP_006520461.1	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X7
XM_006520392.2	XP_006520455.1	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X1
XM_006520395.2	XP_006520458.1	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X4
XM_030248316.1	XP_030104176.1	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X15
XM_030248317.1	XP_030104177.1	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X16
NM_001276467.1	NP_001263396.1	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform e
XM_006520403.3	XP_006520466.1	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X13
XM_006520393.2	XP_006520456.1	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X2
XM_006520399.4	XP_006520462.1	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X8
XM_030248315.1	XP_030104175.1	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X11
NM_001276461.1	NP_001263390.1	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform b
XM_006520401.3	XP_006520464.1	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X11
XM_006520402.3	XP_006520465.1	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X12
XM_006520394.2	XP_006520457.1	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X3
NM_010026.3	NP_034156.2	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform a
NM_001276462.1	NP_001263391.1	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform c
NM_001276463.1	NP_001263392.1	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform d
XM_017316425.1	XP_017171914.1	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X9
XM_006520400.4	XP_006520463.1	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X9
XM_006520403.4	XP_006520466.1	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X13
XM_006520399.3	XP_006520462.1	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X8
XM_006520400.3	XP_006520463.1	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X10
XM_006520396.4	XP_006520459.1	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X5
XM_006520397.2	XP_006520460.1	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X6
XM_006520398.4	XP_006520461.1	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X7
XM_006520401.4	XP_006520464.1	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X10
XM_006520402.4	XP_006520465.1	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X12
XM_011245448.1	XP_011243750.1	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X14

Pathways from BioSystems

Homologies

	ASAP1	XP_001084688.1
Homo sapiens (human)	ASAP1	NP_060952.2
Gallus gallus (chicken)	ASAP1	XP_425945.4
Drosophila melanogaster (fruit fly)	Asap1	NP_001163085.1
Xenopus tropicalis (tropical clawed frog)	asap1	NP_001090735.1
Pan troglodytes (chimpanzee)	ASAP1	XP_003311959.1
Mus musculus (house mouse)	Asap1	NP_034156.2
Danio rerio (zebrafish)	asap1b	XP_005162660.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007726	XP_001687844.1
Canis lupus familiaris (dog)	ASAP1	XP_005627995.1
Bos taurus (cattle)	ASAP1	NP_787015.1
Rattus norvegicus (Norway rat)	Asap1	NP_001037710.1

Gene Ontology
Bibliography

Related articles in PubMed

Erythroblast differentiation at spleen in Q137E mutant ribosomal protein S19 gene knock-in C57BL/6J mice.
Yamanegi K, Yamada N, Nakasho K, Nishiura H
Immunobiology223(1)118-124(2018 Jan)

The Arf GTPase-activating Protein, ASAP1, Binds Nonmuscle Myosin 2A to Control Remodeling of the Actomyosin Network.
Chen PW, Jian X, Heissler SM, Le K, Luo R, Jenkins LM, Nagy A, Moss J, Sellers JR, Randazzo PA
The Journal of biological chemistry291(14)7517-26(2016 Apr)

A pooled shRNA screen for regulators of primary mammary stem and progenitor cells identifies roles for Asap1 and Prox1.
Sheridan JM, Ritchie ME, Best SA, Jiang K, Beck TJ, Vaillant F, Liu K, Dickins RA, Smyth GK, Lindeman GJ, Visvader JE
BMC cancer15221(2015 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

results support the hypothesis that ASAP1 is a positive regulator of NM2A.
Title: The Arf GTPase-activating Protein, ASAP1, Binds Nonmuscle Myosin 2A to Control Remodeling of the Actomyosin Network.

we demonstrate that Prox1 and Asap1 behave as negative regulators of mammary stem/progenitor function. Both of these genes have also been implicated in oncogenesis.
Title: A pooled shRNA screen for regulators of primary mammary stem and progenitor cells identifies roles for Asap1 and Prox1.

ASAP1 is a scaffold bringing together the proteins needed for transport to the cilia including the GTP-binding protein Arf4 & the 2 G proteins of the Rab family--Rab11 & Rab8--linked by the Rab8 guanine nucleotide exchange factor Rabin8.
Title: The Arf GAP ASAP1 provides a platform to regulate Arf4- and Rab11-Rab8-mediated ciliary receptor targeting.

ASAP1 was suggested to negatively regulate lipopolysaccharide (LPS)-induced proinflammatory mediators production through down-regulating LPS signaling
Title: An ADP ribosylation factor-GTPase activating protein negatively regulates the production of proinflammatory mediators in response to lipopolysaccharide.

ASAP3 functions nonredundantly with ASAP1 to control cell movement and may have a role in cancer cell invasion.
Title: ASAP3 is a focal adhesion-associated Arf GAP that functions in cell migration and invasion.

The following Asap1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Asap1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu50917	XM_006520396.4 Latest version!	Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu50919	XM_006520398.4 Latest version!	Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu50920	XM_006520399.4 Latest version!	Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu50921	XM_006520400.4 Latest version!	Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu50922	XM_006520401.4 Latest version!	Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu50923	XM_006520402.4 Latest version!	Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu50924	XM_006520403.4 Latest version!	Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu86631	XM_030248315.1 Latest version!	Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu86632	XM_030248316.1 Latest version!	Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu86633	XM_030248317.1 Latest version!	Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu05418	NM_010026.3 Latest version!	Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
OMu17132	NM_001276467.1 Latest version!	Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
OMu17150	NM_001276462.1 Latest version!	Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
OMu17242	NM_001276463.1 Latest version!	Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
OMu17289	NM_001276461.1 Latest version!	Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
OMu50913	XM_006520392.2 Latest version!	Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OMu50914	XM_006520393.2 Latest version!	Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OMu50915	XM_006520394.2 Latest version!	Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OMu50916	XM_006520395.2 Latest version!	Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OMu50918	XM_006520397.2 Latest version!	Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OMu67522	XM_011245448.1 Latest version!	Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
OMu78998	XM_017316425.1 Latest version!	Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
View Old Accession Versions >>
OMu50924	XM_006520403.3	Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
OMu50920	XM_006520399.3	Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
OMu50921	XM_006520400.3	Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
OMu50917	XM_006520396.3	Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OMu50919	XM_006520398.3	Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
OMu50922	XM_006520401.3	Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
OMu50923	XM_006520402.3	Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Asap1 ( XM_006520396.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006520459.1 Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMu50917
Clone ID Related Accession (Same CDS sequence)	XM_006520396.3 , XM_006520396.4
Accession Version	XM_006520396.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006520396.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCCA GTCATACGCT TTCAAGGGCT CCTGGAGGCC AATCCCAAGG ACTAGGATGC 
CGGACCAGAT CTCCGTGTCC GAGTTCATCG CCGAGACCAC CGAGGACTAC AACTCGCCCA 
CCACGTCCAG CTTCACCACG CGGCTGCACA ACTGCAGGAA CACCGTCACG CTGCTGGAGG 
AGCGTGGTTG ACGGTGGCAG CCGAGCAGTG GTGCAGATGA CGCCAGCTGT GTTGCACTTG 
GGTGAGCCAT ACACAAGTTC ATTTGAGGCT CTAGACCAAG ATAGAACAGC CCTACAGAAA 
GTGAAGAAAT CTGTAAAAGC AATATATAAT TCCGGTCAAG ATCATGTACA AAATGAAGAA 
AACTATGCAC AAGTTCTGGA TAAGTTTGGA AGTAATTTTT TAAGCAGAGA CAACCCTGAC 
CTTGGCACCG CTTTTGTCAA GTTTTCTACA CTCACAAAGG AACTGTCCAC GCTGCTGAAA 
AATCTGCTCC AGGGTCTGAG CCACAATGTG ATCTTCACCT TGGATTCCTT GTTAAAAGGA 
GACTTGAAGG GGGTCAAAGG AGATCTCAAG AAGCCATTTG ACAAAGCCTG GAAAGATTAT 
GAGACGAAGT TTACAAAAAT TGAGAAGGAG AAAAGAGAGC ACGCAAAACA GCACGGGATG 
ATCCGAACAG AGATAACAGG CGCTGAGATT GCAGAAGAAA TGGAAAAGGA ACGGCGGCTT 
TTTCAGCTCC AGATGTGTGA ATATCTCATT AAAGTAAATG AAATCAAGAC CAAAAAGGGT 
GTGGACCTGC TGCAGAACCT TATAAAGTAT TACCATGCAC AGTGCAATTT CTTTCAGGAT 
GGCTTGAAAA CGGCTGATAA ACTGAAACAG TACATAGAAA AGCTGGCTGC TGACTTATAT 
AATATAAAGC AAACCCAAGA TGAAGAAAAA AAACAGCTAA CTGCACTCCG AGATCTAATA 
AAATCCTCTC TGCAACTTGA TCCGAAAGAA GTAGGTGGTT TATATGTTGC CAGCAGGGCT 
AACAGTTCTA GAAGAGACTC TCAGAGCCGG CAAGGTGGGT ACAGCATGCA TCAGCTCCAG 
GGCAACAAGG AGTACGGCAG TGAAAAGAAG GGCTTCCTGC TGAAGAAGAG TGATGGGATC 
CGGAAAGTGT GGCAGAGACG GAAGTGTGCG GTCAAGAACG GGATCCTGAC CATCTCCCAC 
GCAACTTCCA ACAGGCAGCC TGCTAAGTTA AACCTCCTCA CCTGCCAAGT GAAACCGAAT 
GCTGAGGACA AGAAGTCTTT TGACCTGATA TCACATAATC GAACATATCA CTTTCAAGCA 
GAAGATGAGC AGGATTATAT AGCGTGGATA TCAGTACTGA CGAATAGCAA AGAAGAGGCC 
CTAACCATGG CCTTCCGTGG TGAGCAGAGC ACAGGGGAGA ATAGCCTGGA GGACCTTACC 
AAAGCCATCA TCGAGGATGT ACAGCGGCTC CCTGGGAATG ACATCTGCTG TGACTGCGGC 
TCATCAGAAC CCACGTGGCT TTCAACCAAC TTGGGTATTT TGACCTGTAT AGAATGTTCC 
GGAATCCATA GGGAAATGGG GGTTCATATT TCTCGCATTC AGTCTTTGGA ACTAGACAAA 
TTAGGAACTT CCGAACTCTT GCTGGCCAAG AATGTAGGAA ACAATAGTTT TAATGATATT 
ATGGAAGCAA ATTTACCCAG CCCTTCACCA AAGCCCACCC CTTCAAGTGA TATGACCGTG 
CGTAAAGAGT ACATCACTGC AAAGTATGTC GACCACCGCT TCTCCAGGAA GACCTGTGCC 
TCTTCCTCAG CAAAGCTGAA CGAATTGCTT GAGGCCATCA AGTCCAGGGA CTTACTTGCA 
TTAATCCAAG TCTATGCAGA GGGGGTGGAG TTAATGGAGC CACTGCTGGA ACCTGGGCAG 
GAGCTCGGGG AGACAGCCCT TCATCTTGCC GTCAGGACCG CAGACCAGAC ATCTCTCCAT 
CTGGTTGATT TCCTTGTACA AAACTGTGGG AACCTGGATA AGCAAACGTC TGTGGGGAAT 
ACAGTTCTGC ACTATTGCAG CATGTACGGC AAGCCGGAGT GTCTGAAGCT GCTGCTTAGG 
AGCAAGCCCA CCGTGGACAT CGTTAACCAG AATGGAGAAA CTGCCTTGGA TATAGCAAAG 
AGACTCAAAG CTACCCAGTG TGAAGATCTG CTTTCCCAGG CTAAATCTGG AAAGTTCAAC 
CCTCATGTCC ACGTAGAGTA TGAGTGGAAT CTTCGACAGG ACGAGATGGA TGAGAGCGAT 
GACGATCTGG ATGACAAGCC AAGCCCGATC AAAAAGGAAC GCTCGCCCAG ACCACAGAGC 
TTCTGCCACT CCTCCAGCAT CTCGCCACAG GACAAGCTGG CACTGCCAGG GTTCAGCACT 
CCACGGGACA AGCAGCGGCT CTCCTACGGA GCCTTCACCA ACCAGATCTT CGCCTCTACG 
AGCACAGATT TGCCCACATC ACCCACCAGT GAGGCTCCCC CTTTGCCACC TCGGAACGCC 
GGGAAAGGTC CAACTGGCCC ACCTTCAACA CTCCCTCTAG GCACCCAGAC CTCTAGTGGC 
AGCTCCACCC TATCCAAGAA GAGGCCTCCT CCCCCACCAC CAGGACACAA GAGAACCCTG 
TCTGACCCTC CCAGCCCACT ACCTCACGGG CCCCCAAACA AAGGCGCAAT TCCTTGGGGT 
AATGATGTGG GCCCATTATC TTCAAGTAAG ACGGCCAACA AGTTTGAGGG GCTGTCTCAG 
CAAGCAAGCA CCAGTTCTGC TAAGACTGCC CTTGGCCCGA GAGTGCTTCC TAAACTACCT 
CAGAAAGTGG CACTAAGGAA GACGGAGACC AGCCATCATC TCTCCCTCGA CAGAACCAAC 
ATCCCACCTG AGACTTTTCA GAAATCATCA CAGTTGACAG AGTTACCCCA AAAGCCACCA 
CTTGGAGAGC TGCCCCCGAA GCCTGTGGAA CTGGCCCCCA AGCCCCAAGT TGGAGAGCTG 
CCACCTAAGC CTGGAGAGCT ACCCCCTAAG CCCCAATTAG GTGACCTGCC CCCCAAGCCA 
CAGCTCTCAG ACTTACCTCC CAAGCCACAG ATGAAGGACC TGCCCCCTAA GCCGCAGCTG 
GGGGATCTGC TGGCAAAGTC CCAGGCTGGC GATGTCTCAG CCAAGGTGCA GCCACCCTCA 
GAGGTCACAC AGAGGTCACA CACCGGGGAT CTGTCTCCAA ATGTACAGTC CAGAGATGCC 
ATCCAGAAGC AAGCATCTGA AGACTCCAAC GACCTCACAC CCACGCTGCC AGAGACACCC 
GTACCACTGC CGAGAAAAAT CAATACGGGG AAAAATAAAG TGAGGCGGGT GAAGACCATT 
TATGACTGCC AGGCAGATAA TGACGACGAA CTCACATTTA TTGAGGGGGA AGTGATCATT 
GTCACCGGGG AAGAGGACCA GGAGTGGTGG ATCGGGCATA TCGAAGGACA GCCTGAAAGG 
AAGGGTGTCT TTCCAGTGTC CTTTGTCCAC ATCCTGTCTG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006520459.1
CDS	254..3778
Translation	MEASHTLSRAPGGQSQGLGCRTRSPCPSSSPRPPRTTTRPPRPASPRGCTTAGTPSRCWRSVVDGGSRAVVQMTPAVLHLGEPYTSSFEALDQDRTALQKVKKSVKAIYNSGQDHVQNEENYAQVLDKFGSNFLSRDNPDLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGVKGDLKKPFDKAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDPKEVGGLYVASRANSSRRDSQSRQGGYSMHQLQGNKEYGSEKKGFLLKKSDGIRKVWQRRKCAVKNGILTISHATSNRQPAKLNLLTCQVKPNAEDKKSFDLISHNRTYHFQAEDEQDYIAWISVLTNSKEEALTMAFRGEQSTGENSLEDLTKAIIEDVQRLPGNDICCDCGSSEPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDKLGTSELLLAKNVGNNSFNDIMEANLPSPSPKPTPSSDMTVRKEYITAKYVDHRFSRKTCASSSAKLNELLEAIKSRDLLALIQVYAEGVELMEPLLEPGQELGETALHLAVRTADQTSLHLVDFLVQNCGNLDKQTSVGNTVLHYCSMYGKPECLKLLLRSKPTVDIVNQNGETALDIAKRLKATQCEDLLSQAKSGKFNPHVHVEYEWNLRQDEMDESDDDLDDKPSPIKKERSPRPQSFCHSSSISPQDKLALPGFSTPRDKQRLSYGAFTNQIFASTSTDLPTSPTSEAPPLPPRNAGKGPTGPPSTLPLGTQTSSGSSTLSKKRPPPPPPGHKRTLSDPPSPLPHGPPNKGAIPWGNDVGPLSSSKTANKFEGLSQQASTSSAKTALGPRVLPKLPQKVALRKTETSHHLSLDRTNIPPETFQKSSQLTELPQKPPLGELPPKPVELAPKPQVGELPPKPGELPPKPQLGDLPPKPQLSDLPPKPQMKDLPPKPQLGDLLAKSQAGDVSAKVQPPSEVTQRSHTGDLSPNVQSRDAIQKQASEDSNDLTPTLPETPVPLPRKINTGKNKVRRVKTIYDCQADNDDELTFIEGEVIIVTGEEDQEWWIGHIEGQPERKGVFPVSFVHILSD

Target ORF information:

RefSeq Version	XM_006520396.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006520396.4

ORF Insert Sequence:

ATGGAGGCCA GTCATACGCT TTCAAGGGCT CCTGGAGGCC AATCCCAAGG ACTAGGATGC 
CGGACCAGAT CTCCGTGTCC GAGTTCATCG CCGAGACCAC CGAGGACTAC AACTCGCCCA 
CCACGTCCAG CTTCACCACG CGGCTGCACA ACTGCAGGAA CACCGTCACG CTGCTGGAGG 
AGCGTGGTTG ACGGTGGCAG CCGAGCAGTG GTGCAGATGA CGCCAGCTGT GTTGCACTTG 
GGTGAGCCAT ACACAAGTTC ATTTGAGGCT CTAGACCAAG ATAGAACAGC CCTACAGAAA 
GTGAAGAAAT CTGTAAAAGC AATATATAAT TCCGGTCAAG ATCATGTACA AAATGAAGAA 
AACTATGCAC AAGTTCTGGA TAAGTTTGGA AGTAATTTTT TAAGCAGAGA CAACCCTGAC 
CTTGGCACCG CTTTTGTCAA GTTTTCTACA CTCACAAAGG AACTGTCCAC GCTGCTGAAA 
AATCTGCTCC AGGGTCTGAG CCACAATGTG ATCTTCACCT TGGATTCCTT GTTAAAAGGA 
GACTTGAAGG GGGTCAAAGG AGATCTCAAG AAGCCATTTG ACAAAGCCTG GAAAGATTAT 
GAGACGAAGT TTACAAAAAT TGAGAAGGAG AAAAGAGAGC ACGCAAAACA GCACGGGATG 
ATCCGAACAG AGATAACAGG CGCTGAGATT GCAGAAGAAA TGGAAAAGGA ACGGCGGCTT 
TTTCAGCTCC AGATGTGTGA ATATCTCATT AAAGTAAATG AAATCAAGAC CAAAAAGGGT 
GTGGACCTGC TGCAGAACCT TATAAAGTAT TACCATGCAC AGTGCAATTT CTTTCAGGAT 
GGCTTGAAAA CGGCTGATAA ACTGAAACAG TACATAGAAA AGCTGGCTGC TGACTTATAT 
AATATAAAGC AAACCCAAGA TGAAGAAAAA AAACAGCTAA CTGCACTCCG AGATCTAATA 
AAATCCTCTC TGCAACTTGA TCCGAAAGAA GTAGGTGGTT TATATGTTGC CAGCAGGGCT 
AACAGTTCTA GAAGAGACTC TCAGAGCCGG CAAGGTGGGT ACAGCATGCA TCAGCTCCAG 
GGCAACAAGG AGTACGGCAG TGAAAAGAAG GGCTTCCTGC TGAAGAAGAG TGATGGGATC 
CGGAAAGTGT GGCAGAGACG GAAGTGTGCG GTCAAGAACG GGATCCTGAC CATCTCCCAC 
GCAACTTCCA ACAGGCAGCC TGCTAAGTTA AACCTCCTCA CCTGCCAAGT GAAACCGAAT 
GCTGAGGACA AGAAGTCTTT TGACCTGATA TCACATAATC GAACATATCA CTTTCAAGCA 
GAAGATGAGC AGGATTATAT AGCGTGGATA TCAGTACTGA CGAATAGCAA AGAAGAGGCC 
CTAACCATGG CCTTCCGTGG TGAGCAGAGC ACAGGGGAGA ATAGCCTGGA GGACCTTACC 
AAAGCCATCA TCGAGGATGT ACAGCGGCTC CCTGGGAATG ACATCTGCTG TGACTGCGGC 
TCATCAGAAC CCACGTGGCT TTCAACCAAC TTGGGTATTT TGACCTGTAT AGAATGTTCC 
GGAATCCATA GGGAAATGGG GGTTCATATT TCTCGCATTC AGTCTTTGGA ACTAGACAAA 
TTAGGAACTT CCGAACTCTT GCTGGCCAAG AATGTAGGAA ACAATAGTTT TAATGATATT 
ATGGAAGCAA ATTTACCCAG CCCTTCACCA AAGCCCACCC CTTCAAGTGA TATGACCGTG 
CGTAAAGAGT ACATCACTGC AAAGTATGTC GACCACCGCT TCTCCAGGAA GACCTGTGCC 
TCTTCCTCAG CAAAGCTGAA CGAATTGCTT GAGGCCATCA AGTCCAGGGA CTTACTTGCA 
TTAATCCAAG TCTATGCAGA GGGGGTGGAG TTAATGGAGC CACTGCTGGA ACCTGGGCAG 
GAGCTCGGGG AGACAGCCCT TCATCTTGCC GTCAGGACCG CAGACCAGAC ATCTCTCCAT 
CTGGTTGATT TCCTTGTACA AAACTGTGGG AACCTGGATA AGCAAACGTC TGTGGGGAAT 
ACAGTTCTGC ACTATTGCAG CATGTACGGC AAGCCGGAGT GTCTGAAGCT GCTGCTTAGG 
AGCAAGCCCA CCGTGGACAT CGTTAACCAG AATGGAGAAA CTGCCTTGGA TATAGCAAAG 
AGACTCAAAG CTACCCAGTG TGAAGATCTG CTTTCCCAGG CTAAATCTGG AAAGTTCAAC 
CCTCATGTCC ACGTAGAGTA TGAGTGGAAT CTTCGACAGG ACGAGATGGA TGAGAGCGAT 
GACGATCTGG ATGACAAGCC AAGCCCGATC AAAAAGGAAC GCTCGCCCAG ACCACAGAGC 
TTCTGCCACT CCTCCAGCAT CTCGCCACAG GACAAGCTGG CACTGCCAGG GTTCAGCACT 
CCACGGGACA AGCAGCGGCT CTCCTACGGA GCCTTCACCA ACCAGATCTT CGCCTCTACG 
AGCACAGATT TGCCCACATC ACCCACCAGT GAGGCTCCCC CTTTGCCACC TCGGAACGCC 
GGGAAAGGTC CAACTGGCCC ACCTTCAACA CTCCCTCTAG GCACCCAGAC CTCTAGTGGC 
AGCTCCACCC TATCCAAGAA GAGGCCTCCT CCCCCACCAC CAGGACACAA GAGAACCCTG 
TCTGACCCTC CCAGCCCACT ACCTCACGGG CCCCCAAACA AAGGCGCAAT TCCTTGGGGT 
AATGATGTGG GCCCATTATC TTCAAGTAAG ACGGCCAACA AGTTTGAGGG GCTGTCTCAG 
CAAGCAAGCA CCAGTTCTGC TAAGACTGCC CTTGGCCCGA GAGTGCTTCC TAAACTACCT 
CAGAAAGTGG CACTAAGGAA GACGGAGACC AGCCATCATC TCTCCCTCGA CAGAACCAAC 
ATCCCACCTG AGACTTTTCA GAAATCATCA CAGTTGACAG AGTTACCCCA AAAGCCACCA 
CTTGGAGAGC TGCCCCCGAA GCCTGTGGAA CTGGCCCCCA AGCCCCAAGT TGGAGAGCTG 
CCACCTAAGC CTGGAGAGCT ACCCCCTAAG CCCCAATTAG GTGACCTGCC CCCCAAGCCA 
CAGCTCTCAG ACTTACCTCC CAAGCCACAG ATGAAGGACC TGCCCCCTAA GCCGCAGCTG 
GGGGATCTGC TGGCAAAGTC CCAGGCTGGC GATGTCTCAG CCAAGGTGCA GCCACCCTCA 
GAGGTCACAC AGAGGTCACA CACCGGGGAT CTGTCTCCAA ATGTACAGTC CAGAGATGCC 
ATCCAGAAGC AAGCATCTGA AGACTCCAAC GACCTCACAC CCACGCTGCC AGAGACACCC 
GTACCACTGC CGAGAAAAAT CAATACGGGG AAAAATAAAG TGAGGCGGGT GAAGACCATT 
TATGACTGCC AGGCAGATAA TGACGACGAA CTCACATTTA TTGAGGGGGA AGTGATCATT 
GTCACCGGGG AAGAGGACCA GGAGTGGTGG ATCGGGCATA TCGAAGGACA GCCTGAAAGG 
AAGGGTGTCT TTCCAGTGTC CTTTGTCCAC ATCCTGTCTG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asap1 ( XM_006520398.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006520461.1 Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X7, mRNA.

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CloneID	OMu50919
Clone ID Related Accession (Same CDS sequence)	XM_006520398.3 , XM_006520398.4
Accession Version	XM_006520398.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006520398.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCAG GTCTGAGTGG GCCACAGGGC TTGCCTTCCA AAGCAGGCTT TAATGGAGGC 
CAGTCATACG CTTTCAAGGG CTCCTGGAGG CCAATCCCAA GGACTAGGAT GCCGGACCAG 
ATCTCCGTGT CCGAGTTCAT CGCCGAGACC ACCGAGGACT ACAACTCGCC CACCACGTCC 
AGCTTCACCA CGCGGCTGCA CAACTGCAGG AACACCGTCA CGCTGCTGGA GGAGGCTCTA 
GACCAAGATA GAACAGCCCT ACAGAAAGTG AAGAAATCTG TAAAAGCAAT ATATAATTCC 
GGTCAAGATC ATGTACAAAA TGAAGAAAAC TATGCACAAG TTCTGGATAA GTTTGGAAGT 
AATTTTTTAA GCAGAGACAA CCCTGACCTT GGCACCGCTT TTGTCAAGTT TTCTACACTC 
ACAAAGGAAC TGTCCACGCT GCTGAAAAAT CTGCTCCAGG GTCTGAGCCA CAATGTGATC 
TTCACCTTGG ATTCCTTGTT AAAAGGAGAC TTGAAGGGGG TCAAAGGAGA TCTCAAGAAG 
CCATTTGACA AAGCCTGGAA AGATTATGAG ACGAAGTTTA CAAAAATTGA GAAGGAGAAA 
AGAGAGCACG CAAAACAGCA CGGGATGATC CGAACAGAGA TAACAGGCGC TGAGATTGCA 
GAAGAAATGG AAAAGGAACG GCGGCTTTTT CAGCTCCAGA TGTGTGAATA TCTCATTAAA 
GTAAATGAAA TCAAGACCAA AAAGGGTGTG GACCTGCTGC AGAACCTTAT AAAGTATTAC 
CATGCACAGT GCAATTTCTT TCAGGATGGC TTGAAAACGG CTGATAAACT GAAACAGTAC 
ATAGAAAAGC TGGCTGCTGA CTTATATAAT ATAAAGCAAA CCCAAGATGA AGAAAAAAAA 
CAGCTAACTG CACTCCGAGA TCTAATAAAA TCCTCTCTGC AACTTGATCC GAAAGAAGTA 
GGTGGTTTAT ATGTTGCCAG CAGGGCTAAC AGTTCTAGAA GAGACTCTCA GAGCCGGCAA 
GGTGGGTACA GCATGCATCA GCTCCAGGGC AACAAGGAGT ACGGCAGTGA AAAGAAGGGC 
TTCCTGCTGA AGAAGAGTGA TGGGATCCGG AAAGTGTGGC AGAGACGGAA GTGTGCGGTC 
AAGAACGGGA TCCTGACCAT CTCCCACGCA ACTTCCAACA GGCAGCCTGC TAAGTTAAAC 
CTCCTCACCT GCCAAGTGAA ACCGAATGCT GAGGACAAGA AGTCTTTTGA CCTGATATCA 
CATAATCGAA CATATCACTT TCAAGCAGAA GATGAGCAGG ATTATATAGC GTGGATATCA 
GTACTGACGA ATAGCAAAGA AGAGGCCCTA ACCATGGCCT TCCGTGGTGA GCAGAGCACA 
GGGGAGAATA GCCTGGAGGA CCTTACCAAA GCCATCATCG AGGATGTACA GCGGCTCCCT 
GGGAATGACA TCTGCTGTGA CTGCGGCTCA TCAGAACCCA CGTGGCTTTC AACCAACTTG 
GGTATTTTGA CCTGTATAGA ATGTTCCGGA ATCCATAGGG AAATGGGGGT TCATATTTCT 
CGCATTCAGT CTTTGGAACT AGACAAATTA GGAACTTCCG AACTCTTGCT GGCCAAGAAT 
GTAGGAAACA ATAGTTTTAA TGATATTATG GAAGCAAATT TACCCAGCCC TTCACCAAAG 
CCCACCCCTT CAAGTGATAT GACCGTGCGT AAAGAGTACA TCACTGCAAA GTATGTCGAC 
CACCGCTTCT CCAGGAAGAC CTGTGCCTCT TCCTCAGCAA AGCTGAACGA ATTGCTTGAG 
GCCATCAAGT CCAGGGACTT ACTTGCATTA ATCCAAGTCT ATGCAGAGGG GGTGGAGTTA 
ATGGAGCCAC TGCTGGAACC TGGGCAGGAG CTCGGGGAGA CAGCCCTTCA TCTTGCCGTC 
AGGACCGCAG ACCAGACATC TCTCCATCTG GTTGATTTCC TTGTACAAAA CTGTGGGAAC 
CTGGATAAGC AAACGTCTGT GGGGAATACA GTTCTGCACT ATTGCAGCAT GTACGGCAAG 
CCGGAGTGTC TGAAGCTGCT GCTTAGGAGC AAGCCCACCG TGGACATCGT TAACCAGAAT 
GGAGAAACTG CCTTGGATAT AGCAAAGAGA CTCAAAGCTA CCCAGTGTGA AGATCTGCTT 
TCCCAGGCTA AATCTGGAAA GTTCAACCCT CATGTCCACG TAGAGTATGA GTGGAATCTT 
CGACAGGACG AGATGGATGA GAGCGATGAC GATCTGGATG ACAAGCCAAG CCCGATCAAA 
AAGGAACGCT CGCCCAGACC ACAGAGCTTC TGCCACTCCT CCAGCATCTC GCCACAGGAC 
AAGCTGGCAC TGCCAGGGTT CAGCACTCCA CGGGACAAGC AGCGGCTCTC CTACGGAGCC 
TTCACCAACC AGATCTTCGC CTCTACGAGC ACAGATTTGC CCACATCACC CACCAGTGAG 
GCTCCCCCTT TGCCACCTCG GAACGCCGGG AAAGGTCCAA CTGGCCCACC TTCAACACTC 
CCTCTAGGCA CCCAGACCTC TAGTGGCAGC TCCACCCTAT CCAAGAAGAG GCCTCCTCCC 
CCACCACCAG GACACAAGAG AACCCTGTCT GACCCTCCCA GCCCACTACC TCACGGGCCC 
CCAAACAAAG GCGCAATTCC TTGGGGTAAT GATGTGGGCC CATTATCTTC AAGTAAGACG 
GCCAACAAGT TTGAGGGGCT GTCTCAGCAA GCAAGCACCA GTTCTGCTAA GACTGCCCTT 
GGCCCGAGAG TGCTTCCTAA ACTACCTCAG AAAGTGGCAC TAAGGAAGAC GGAGACCAGC 
CATCATCTCT CCCTCGACAG AACCAACATC CCACCTGAGA CTTTTCAGAA ATCATCACAG 
TTGACAGAGT TACCCCAAAA GCCACCACTT GGAGAGCTGC CCCCGAAGCC TGTGGAACTG 
GCCCCCAAGC CCCAAGTTGG AGAGCTGCCA CCTAAGCCTG GAGAGCTACC CCCTAAGCCC 
CAATTAGGTG ACCTGCCCCC CAAGCCACAG CTCTCAGACT TACCTCCCAA GCCACAGATG 
AAGGACCTGC CCCCTAAGCC GCAGCTGGGG GATCTGCTGG CAAAGTCCCA GGCTGGCGAT 
GTCTCAGCCA AGGTGCAGCC ACCCTCAGAG GTCACACAGA GGTCACACAC CGGGGATCTG 
TCTCCAAATG TACAGTCCAG AGATGCCATC CAGAAGCAAG CATCTGAAGA CTCCAACGAC 
CTCACACCCA CGCTGCCAGA GACACCCGTA CCACTGCCGA GAAAAATCAA TACGGGGAAA 
AATAAAGTGA GGCGGGTGAA GACCATTTAT GACTGCCAGG CAGATAATGA CGACGAACTC 
ACATTTATTG AGGGGGAAGT GATCATTGTC ACCGGGGAAG AGGACCAGGA GTGGTGGATC 
GGGCATATCG AAGGACAGCC TGAAAGGAAG GGTGTCTTTC CAGTGTCCTT TGTCCACATC 
CTGTCTGACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006520461.1
CDS	356..3847
Translation	MQAGLSGPQGLPSKAGFNGGQSYAFKGSWRPIPRTRMPDQISVSEFIAETTEDYNSPTTSSFTTRLHNCRNTVTLLEEALDQDRTALQKVKKSVKAIYNSGQDHVQNEENYAQVLDKFGSNFLSRDNPDLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGVKGDLKKPFDKAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDPKEVGGLYVASRANSSRRDSQSRQGGYSMHQLQGNKEYGSEKKGFLLKKSDGIRKVWQRRKCAVKNGILTISHATSNRQPAKLNLLTCQVKPNAEDKKSFDLISHNRTYHFQAEDEQDYIAWISVLTNSKEEALTMAFRGEQSTGENSLEDLTKAIIEDVQRLPGNDICCDCGSSEPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDKLGTSELLLAKNVGNNSFNDIMEANLPSPSPKPTPSSDMTVRKEYITAKYVDHRFSRKTCASSSAKLNELLEAIKSRDLLALIQVYAEGVELMEPLLEPGQELGETALHLAVRTADQTSLHLVDFLVQNCGNLDKQTSVGNTVLHYCSMYGKPECLKLLLRSKPTVDIVNQNGETALDIAKRLKATQCEDLLSQAKSGKFNPHVHVEYEWNLRQDEMDESDDDLDDKPSPIKKERSPRPQSFCHSSSISPQDKLALPGFSTPRDKQRLSYGAFTNQIFASTSTDLPTSPTSEAPPLPPRNAGKGPTGPPSTLPLGTQTSSGSSTLSKKRPPPPPPGHKRTLSDPPSPLPHGPPNKGAIPWGNDVGPLSSSKTANKFEGLSQQASTSSAKTALGPRVLPKLPQKVALRKTETSHHLSLDRTNIPPETFQKSSQLTELPQKPPLGELPPKPVELAPKPQVGELPPKPGELPPKPQLGDLPPKPQLSDLPPKPQMKDLPPKPQLGDLLAKSQAGDVSAKVQPPSEVTQRSHTGDLSPNVQSRDAIQKQASEDSNDLTPTLPETPVPLPRKINTGKNKVRRVKTIYDCQADNDDELTFIEGEVIIVTGEEDQEWWIGHIEGQPERKGVFPVSFVHILSD

Target ORF information:

RefSeq Version	XM_006520398.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006520398.4

ORF Insert Sequence:

ATGCAGGCAG GTCTGAGTGG GCCACAGGGC TTGCCTTCCA AAGCAGGCTT TAATGGAGGC 
CAGTCATACG CTTTCAAGGG CTCCTGGAGG CCAATCCCAA GGACTAGGAT GCCGGACCAG 
ATCTCCGTGT CCGAGTTCAT CGCCGAGACC ACCGAGGACT ACAACTCGCC CACCACGTCC 
AGCTTCACCA CGCGGCTGCA CAACTGCAGG AACACCGTCA CGCTGCTGGA GGAGGCTCTA 
GACCAAGATA GAACAGCCCT ACAGAAAGTG AAGAAATCTG TAAAAGCAAT ATATAATTCC 
GGTCAAGATC ATGTACAAAA TGAAGAAAAC TATGCACAAG TTCTGGATAA GTTTGGAAGT 
AATTTTTTAA GCAGAGACAA CCCTGACCTT GGCACCGCTT TTGTCAAGTT TTCTACACTC 
ACAAAGGAAC TGTCCACGCT GCTGAAAAAT CTGCTCCAGG GTCTGAGCCA CAATGTGATC 
TTCACCTTGG ATTCCTTGTT AAAAGGAGAC TTGAAGGGGG TCAAAGGAGA TCTCAAGAAG 
CCATTTGACA AAGCCTGGAA AGATTATGAG ACGAAGTTTA CAAAAATTGA GAAGGAGAAA 
AGAGAGCACG CAAAACAGCA CGGGATGATC CGAACAGAGA TAACAGGCGC TGAGATTGCA 
GAAGAAATGG AAAAGGAACG GCGGCTTTTT CAGCTCCAGA TGTGTGAATA TCTCATTAAA 
GTAAATGAAA TCAAGACCAA AAAGGGTGTG GACCTGCTGC AGAACCTTAT AAAGTATTAC 
CATGCACAGT GCAATTTCTT TCAGGATGGC TTGAAAACGG CTGATAAACT GAAACAGTAC 
ATAGAAAAGC TGGCTGCTGA CTTATATAAT ATAAAGCAAA CCCAAGATGA AGAAAAAAAA 
CAGCTAACTG CACTCCGAGA TCTAATAAAA TCCTCTCTGC AACTTGATCC GAAAGAAGTA 
GGTGGTTTAT ATGTTGCCAG CAGGGCTAAC AGTTCTAGAA GAGACTCTCA GAGCCGGCAA 
GGTGGGTACA GCATGCATCA GCTCCAGGGC AACAAGGAGT ACGGCAGTGA AAAGAAGGGC 
TTCCTGCTGA AGAAGAGTGA TGGGATCCGG AAAGTGTGGC AGAGACGGAA GTGTGCGGTC 
AAGAACGGGA TCCTGACCAT CTCCCACGCA ACTTCCAACA GGCAGCCTGC TAAGTTAAAC 
CTCCTCACCT GCCAAGTGAA ACCGAATGCT GAGGACAAGA AGTCTTTTGA CCTGATATCA 
CATAATCGAA CATATCACTT TCAAGCAGAA GATGAGCAGG ATTATATAGC GTGGATATCA 
GTACTGACGA ATAGCAAAGA AGAGGCCCTA ACCATGGCCT TCCGTGGTGA GCAGAGCACA 
GGGGAGAATA GCCTGGAGGA CCTTACCAAA GCCATCATCG AGGATGTACA GCGGCTCCCT 
GGGAATGACA TCTGCTGTGA CTGCGGCTCA TCAGAACCCA CGTGGCTTTC AACCAACTTG 
GGTATTTTGA CCTGTATAGA ATGTTCCGGA ATCCATAGGG AAATGGGGGT TCATATTTCT 
CGCATTCAGT CTTTGGAACT AGACAAATTA GGAACTTCCG AACTCTTGCT GGCCAAGAAT 
GTAGGAAACA ATAGTTTTAA TGATATTATG GAAGCAAATT TACCCAGCCC TTCACCAAAG 
CCCACCCCTT CAAGTGATAT GACCGTGCGT AAAGAGTACA TCACTGCAAA GTATGTCGAC 
CACCGCTTCT CCAGGAAGAC CTGTGCCTCT TCCTCAGCAA AGCTGAACGA ATTGCTTGAG 
GCCATCAAGT CCAGGGACTT ACTTGCATTA ATCCAAGTCT ATGCAGAGGG GGTGGAGTTA 
ATGGAGCCAC TGCTGGAACC TGGGCAGGAG CTCGGGGAGA CAGCCCTTCA TCTTGCCGTC 
AGGACCGCAG ACCAGACATC TCTCCATCTG GTTGATTTCC TTGTACAAAA CTGTGGGAAC 
CTGGATAAGC AAACGTCTGT GGGGAATACA GTTCTGCACT ATTGCAGCAT GTACGGCAAG 
CCGGAGTGTC TGAAGCTGCT GCTTAGGAGC AAGCCCACCG TGGACATCGT TAACCAGAAT 
GGAGAAACTG CCTTGGATAT AGCAAAGAGA CTCAAAGCTA CCCAGTGTGA AGATCTGCTT 
TCCCAGGCTA AATCTGGAAA GTTCAACCCT CATGTCCACG TAGAGTATGA GTGGAATCTT 
CGACAGGACG AGATGGATGA GAGCGATGAC GATCTGGATG ACAAGCCAAG CCCGATCAAA 
AAGGAACGCT CGCCCAGACC ACAGAGCTTC TGCCACTCCT CCAGCATCTC GCCACAGGAC 
AAGCTGGCAC TGCCAGGGTT CAGCACTCCA CGGGACAAGC AGCGGCTCTC CTACGGAGCC 
TTCACCAACC AGATCTTCGC CTCTACGAGC ACAGATTTGC CCACATCACC CACCAGTGAG 
GCTCCCCCTT TGCCACCTCG GAACGCCGGG AAAGGTCCAA CTGGCCCACC TTCAACACTC 
CCTCTAGGCA CCCAGACCTC TAGTGGCAGC TCCACCCTAT CCAAGAAGAG GCCTCCTCCC 
CCACCACCAG GACACAAGAG AACCCTGTCT GACCCTCCCA GCCCACTACC TCACGGGCCC 
CCAAACAAAG GCGCAATTCC TTGGGGTAAT GATGTGGGCC CATTATCTTC AAGTAAGACG 
GCCAACAAGT TTGAGGGGCT GTCTCAGCAA GCAAGCACCA GTTCTGCTAA GACTGCCCTT 
GGCCCGAGAG TGCTTCCTAA ACTACCTCAG AAAGTGGCAC TAAGGAAGAC GGAGACCAGC 
CATCATCTCT CCCTCGACAG AACCAACATC CCACCTGAGA CTTTTCAGAA ATCATCACAG 
TTGACAGAGT TACCCCAAAA GCCACCACTT GGAGAGCTGC CCCCGAAGCC TGTGGAACTG 
GCCCCCAAGC CCCAAGTTGG AGAGCTGCCA CCTAAGCCTG GAGAGCTACC CCCTAAGCCC 
CAATTAGGTG ACCTGCCCCC CAAGCCACAG CTCTCAGACT TACCTCCCAA GCCACAGATG 
AAGGACCTGC CCCCTAAGCC GCAGCTGGGG GATCTGCTGG CAAAGTCCCA GGCTGGCGAT 
GTCTCAGCCA AGGTGCAGCC ACCCTCAGAG GTCACACAGA GGTCACACAC CGGGGATCTG 
TCTCCAAATG TACAGTCCAG AGATGCCATC CAGAAGCAAG CATCTGAAGA CTCCAACGAC 
CTCACACCCA CGCTGCCAGA GACACCCGTA CCACTGCCGA GAAAAATCAA TACGGGGAAA 
AATAAAGTGA GGCGGGTGAA GACCATTTAT GACTGCCAGG CAGATAATGA CGACGAACTC 
ACATTTATTG AGGGGGAAGT GATCATTGTC ACCGGGGAAG AGGACCAGGA GTGGTGGATC 
GGGCATATCG AAGGACAGCC TGAAAGGAAG GGTGTCTTTC CAGTGTCCTT TGTCCACATC 
CTGTCTGACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asap1 ( XM_006520399.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006520462.1 Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OMu50920
Clone ID Related Accession (Same CDS sequence)	XM_006520399.4 , XM_006520399.3
Accession Version	XM_006520399.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006520399.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCAG GTCTGAGTGG GCCACAGGGC TTGCCTTCCA AAGCAGGCTT TAATGGAGGC 
CAGTCATACG CTTTCAAGGG CTCCTGGAGG CCAATCCCAA GGACTAGGAT GCCGGACCAG 
ATCTCCGTGT CCGAGTTCAT CGCCGAGACC ACCGAGGACT ACAACTCGCC CACCACGTCC 
AGCTTCACCA CGCGGCTGCA CAACTGCAGG AACACCGTCA CGCTGCTGGA GGAGGCTCTA 
GACCAAGATA GAACAGCCCT ACAGAAAGTG AAGAAATCTG TAAAAGCAAT ATATAATTCC 
GGTCAAGATC ATGTACAAAA TGAAGAAAAC TATGCACAAG TTCTGGATAA GTTTGGAAGT 
AATTTTTTAA GCAGAGACAA CCCTGACCTT GGCACCGCTT TTGTCAAGTT TTCTACACTC 
ACAAAGGAAC TGTCCACGCT GCTGAAAAAT CTGCTCCAGG GTCTGAGCCA CAATGTGATC 
TTCACCTTGG ATTCCTTGTT AAAAGGAGAC TTGAAGGGGG TCAAAGGAGA TCTCAAGAAG 
CCATTTGACA AAGCCTGGAA AGATTATGAG ACGAAGTTTA CAAAAATTGA GAAGGAGAAA 
AGAGAGCACG CAAAACAGCA CGGGATGATC CGAACAGAGA TAACAGGCGC TGAGATTGCA 
GAAGAAATGG AAAAGGAACG GCGGCTTTTT CAGCTCCAGA TGTGTGAATA TCTCATTAAA 
GTAAATGAAA TCAAGACCAA AAAGGGTGTG GACCTGCTGC AGAACCTTAT AAAGTATTAC 
CATGCACAGT GCAATTTCTT TCAGGATGGC TTGAAAACGG CTGATAAACT GAAACAGTAC 
ATAGAAAAGC TGGCTGCTGA CTTATATAAT ATAAAGCAAA CCCAAGATGA AGAAAAAAAA 
CAGCTAACTG CACTCCGAGA TCTAATAAAA TCCTCTCTGC AACTTGATCC GAAAGAAGTA 
GGTGGTTTAT ATGTTGCCAG CAGGGCTAAC AGTGACTCTC AGAGCCGGCA AGGTGGGTAC 
AGCATGCATC AGCTCCAGGG CAACAAGGAG TACGGCAGTG AAAAGAAGGG CTTCCTGCTG 
AAGAAGAGTG ATGGGATCCG GAAAGTGTGG CAGAGACGGA AGTGTGCGGT CAAGAACGGG 
ATCCTGACCA TCTCCCACGC AACTTCCAAC AGGCAGCCTG CTAAGTTAAA CCTCCTCACC 
TGCCAAGTGA AACCGAATGC TGAGGACAAG AAGTCTTTTG ACCTGATATC ACATAATCGA 
ACATATCACT TTCAAGCAGA AGATGAGCAG GATTATATAG CGTGGATATC AGTACTGACG 
AATAGCAAAG AAGAGGCCCT AACCATGGCC TTCCGTGGTG AGCAGAGCAC AGGGGAGAAT 
AGCCTGGAGG ACCTTACCAA AGCCATCATC GAGGATGTAC AGCGGCTCCC TGGGAATGAC 
ATCTGCTGTG ACTGCGGCTC ATCAGAACCC ACGTGGCTTT CAACCAACTT GGGTATTTTG 
ACCTGTATAG AATGTTCCGG AATCCATAGG GAAATGGGGG TTCATATTTC TCGCATTCAG 
TCTTTGGAAC TAGACAAATT AGGAACTTCC GAACTCTTGC TGGCCAAGAA TGTAGGAAAC 
AATAGTTTTA ATGATATTAT GGAAGCAAAT TTACCCAGCC CTTCACCAAA GCCCACCCCT 
TCAAGTGATA TGACCGTGCG TAAAGAGTAC ATCACTGCAA AGTATGTCGA CCACCGCTTC 
TCCAGGAAGA CCTGTGCCTC TTCCTCAGCA AAGCTGAACG AATTGCTTGA GGCCATCAAG 
TCCAGGGACT TACTTGCATT AATCCAAGTC TATGCAGAGG GGGTGGAGTT AATGGAGCCA 
CTGCTGGAAC CTGGGCAGGA GCTCGGGGAG ACAGCCCTTC ATCTTGCCGT CAGGACCGCA 
GACCAGACAT CTCTCCATCT GGTTGATTTC CTTGTACAAA ACTGTGGGAA CCTGGATAAG 
CAAACGTCTG TGGGGAATAC AGTTCTGCAC TATTGCAGCA TGTACGGCAA GCCGGAGTGT 
CTGAAGCTGC TGCTTAGGAG CAAGCCCACC GTGGACATCG TTAACCAGAA TGGAGAAACT 
GCCTTGGATA TAGCAAAGAG ACTCAAAGCT ACCCAGTGTG AAGATCTGCT TTCCCAGGCT 
AAATCTGGAA AGTTCAACCC TCATGTCCAC GTAGAGTATG AGTGGAATCT TCGACAGGAC 
GAGATGGATG AGAGCGATGA CGATCTGGAT GACAAGCCAA GCCCGATCAA AAAGGAACGC 
TCGCCCAGAC CACAGAGCTT CTGCCACTCC TCCAGCATCT CGCCACAGGA CAAGCTGGCA 
CTGCCAGGGT TCAGCACTCC ACGGGACAAG CAGCGGCTCT CCTACGGAGC CTTCACCAAC 
CAGATCTTCG CCTCTACGAG CACAGATTTG CCCACATCAC CCACCAGTGA GGCTCCCCCT 
TTGCCACCTC GGAACGCCGG GAAAGGTCCA ACTGGCCCAC CTTCAACACT CCCTCTAGGC 
ACCCAGACCT CTAGTGGCAG CTCCACCCTA TCCAAGAAGA GGCCTCCTCC CCCACCACCA 
GGACACAAGA GAACCCTGTC TGACCCTCCC AGCCCACTAC CTCACGGGCC CCCAAACAAA 
GGCGCAATTC CTTGGGGTAA TGATGTGGGC CCATTATCTT CAAGTAAGAC GGCCAACAAG 
TTTGAGGGGC TGTCTCAGCA AGCAAGCACC AGTTCTGCTA AGACTGCCCT TGGCCCGAGA 
GTGCTTCCTA AACTACCTCA GAAAGTGGCA CTAAGGAAGA CGGAGACCAG CCATCATCTC 
TCCCTCGACA GAACCAACAT CCCACCTGAG ACTTTTCAGA AATCATCACA GTTGACAGAG 
TTACCCCAAA AGCCACCACT TGGAGAGCTG CCCCCGAAGC CTGTGGAACT GGCCCCCAAG 
CCCCAAGTTG GAGAGCTGCC ACCTAAGCCT GGAGAGCTAC CCCCTAAGCC CCAATTAGGT 
GACCTGCCCC CCAAGCCACA GCTCTCAGAC TTACCTCCCA AGCCACAGAT GAAGGACCTG 
CCCCCTAAGC CGCAGCTGGG GGATCTGCTG GCAAAGTCCC AGGCTGGCGA TGTCTCAGCC 
AAGGTGCAGC CACCCTCAGA GGTCACACAG AGGTCACACA CCGGGGATCT GTCTCCAAAT 
GTACAGTCCA GAGATGCCAT CCAGAAGCAA GCATCTGAAG ACTCCAACGA CCTCACACCC 
ACGCTGCCAG AGACACCCGT ACCACTGCCG AGAAAAATCA ATACGGGGAA AAATAAAGTG 
AGGCGGGTGA AGACCATTTA TGACTGCCAG GCAGATAATG ACGACGAACT CACATTTATT 
GAGGGGGAAG TGATCATTGT CACCGGGGAA GAGGACCAGG AGTGGTGGAT CGGGCATATC 
GAAGGACAGC CTGAAAGGAA GGGTGTCTTT CCAGTGTCCT TTGTCCACAT CCTGTCTGAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006520462.1
CDS	202..3684
Translation	MQAGLSGPQGLPSKAGFNGGQSYAFKGSWRPIPRTRMPDQISVSEFIAETTEDYNSPTTSSFTTRLHNCRNTVTLLEEALDQDRTALQKVKKSVKAIYNSGQDHVQNEENYAQVLDKFGSNFLSRDNPDLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGVKGDLKKPFDKAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDPKEVGGLYVASRANSDSQSRQGGYSMHQLQGNKEYGSEKKGFLLKKSDGIRKVWQRRKCAVKNGILTISHATSNRQPAKLNLLTCQVKPNAEDKKSFDLISHNRTYHFQAEDEQDYIAWISVLTNSKEEALTMAFRGEQSTGENSLEDLTKAIIEDVQRLPGNDICCDCGSSEPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDKLGTSELLLAKNVGNNSFNDIMEANLPSPSPKPTPSSDMTVRKEYITAKYVDHRFSRKTCASSSAKLNELLEAIKSRDLLALIQVYAEGVELMEPLLEPGQELGETALHLAVRTADQTSLHLVDFLVQNCGNLDKQTSVGNTVLHYCSMYGKPECLKLLLRSKPTVDIVNQNGETALDIAKRLKATQCEDLLSQAKSGKFNPHVHVEYEWNLRQDEMDESDDDLDDKPSPIKKERSPRPQSFCHSSSISPQDKLALPGFSTPRDKQRLSYGAFTNQIFASTSTDLPTSPTSEAPPLPPRNAGKGPTGPPSTLPLGTQTSSGSSTLSKKRPPPPPPGHKRTLSDPPSPLPHGPPNKGAIPWGNDVGPLSSSKTANKFEGLSQQASTSSAKTALGPRVLPKLPQKVALRKTETSHHLSLDRTNIPPETFQKSSQLTELPQKPPLGELPPKPVELAPKPQVGELPPKPGELPPKPQLGDLPPKPQLSDLPPKPQMKDLPPKPQLGDLLAKSQAGDVSAKVQPPSEVTQRSHTGDLSPNVQSRDAIQKQASEDSNDLTPTLPETPVPLPRKINTGKNKVRRVKTIYDCQADNDDELTFIEGEVIIVTGEEDQEWWIGHIEGQPERKGVFPVSFVHILSD

Target ORF information:

RefSeq Version	XM_006520399.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006520399.4

ORF Insert Sequence:

ATGCAGGCAG GTCTGAGTGG GCCACAGGGC TTGCCTTCCA AAGCAGGCTT TAATGGAGGC 
CAGTCATACG CTTTCAAGGG CTCCTGGAGG CCAATCCCAA GGACTAGGAT GCCGGACCAG 
ATCTCCGTGT CCGAGTTCAT CGCCGAGACC ACCGAGGACT ACAACTCGCC CACCACGTCC 
AGCTTCACCA CGCGGCTGCA CAACTGCAGG AACACCGTCA CGCTGCTGGA GGAGGCTCTA 
GACCAAGATA GAACAGCCCT ACAGAAAGTG AAGAAATCTG TAAAAGCAAT ATATAATTCC 
GGTCAAGATC ATGTACAAAA TGAAGAAAAC TATGCACAAG TTCTGGATAA GTTTGGAAGT 
AATTTTTTAA GCAGAGACAA CCCTGACCTT GGCACCGCTT TTGTCAAGTT TTCTACACTC 
ACAAAGGAAC TGTCCACGCT GCTGAAAAAT CTGCTCCAGG GTCTGAGCCA CAATGTGATC 
TTCACCTTGG ATTCCTTGTT AAAAGGAGAC TTGAAGGGGG TCAAAGGAGA TCTCAAGAAG 
CCATTTGACA AAGCCTGGAA AGATTATGAG ACGAAGTTTA CAAAAATTGA GAAGGAGAAA 
AGAGAGCACG CAAAACAGCA CGGGATGATC CGAACAGAGA TAACAGGCGC TGAGATTGCA 
GAAGAAATGG AAAAGGAACG GCGGCTTTTT CAGCTCCAGA TGTGTGAATA TCTCATTAAA 
GTAAATGAAA TCAAGACCAA AAAGGGTGTG GACCTGCTGC AGAACCTTAT AAAGTATTAC 
CATGCACAGT GCAATTTCTT TCAGGATGGC TTGAAAACGG CTGATAAACT GAAACAGTAC 
ATAGAAAAGC TGGCTGCTGA CTTATATAAT ATAAAGCAAA CCCAAGATGA AGAAAAAAAA 
CAGCTAACTG CACTCCGAGA TCTAATAAAA TCCTCTCTGC AACTTGATCC GAAAGAAGTA 
GGTGGTTTAT ATGTTGCCAG CAGGGCTAAC AGTGACTCTC AGAGCCGGCA AGGTGGGTAC 
AGCATGCATC AGCTCCAGGG CAACAAGGAG TACGGCAGTG AAAAGAAGGG CTTCCTGCTG 
AAGAAGAGTG ATGGGATCCG GAAAGTGTGG CAGAGACGGA AGTGTGCGGT CAAGAACGGG 
ATCCTGACCA TCTCCCACGC AACTTCCAAC AGGCAGCCTG CTAAGTTAAA CCTCCTCACC 
TGCCAAGTGA AACCGAATGC TGAGGACAAG AAGTCTTTTG ACCTGATATC ACATAATCGA 
ACATATCACT TTCAAGCAGA AGATGAGCAG GATTATATAG CGTGGATATC AGTACTGACG 
AATAGCAAAG AAGAGGCCCT AACCATGGCC TTCCGTGGTG AGCAGAGCAC AGGGGAGAAT 
AGCCTGGAGG ACCTTACCAA AGCCATCATC GAGGATGTAC AGCGGCTCCC TGGGAATGAC 
ATCTGCTGTG ACTGCGGCTC ATCAGAACCC ACGTGGCTTT CAACCAACTT GGGTATTTTG 
ACCTGTATAG AATGTTCCGG AATCCATAGG GAAATGGGGG TTCATATTTC TCGCATTCAG 
TCTTTGGAAC TAGACAAATT AGGAACTTCC GAACTCTTGC TGGCCAAGAA TGTAGGAAAC 
AATAGTTTTA ATGATATTAT GGAAGCAAAT TTACCCAGCC CTTCACCAAA GCCCACCCCT 
TCAAGTGATA TGACCGTGCG TAAAGAGTAC ATCACTGCAA AGTATGTCGA CCACCGCTTC 
TCCAGGAAGA CCTGTGCCTC TTCCTCAGCA AAGCTGAACG AATTGCTTGA GGCCATCAAG 
TCCAGGGACT TACTTGCATT AATCCAAGTC TATGCAGAGG GGGTGGAGTT AATGGAGCCA 
CTGCTGGAAC CTGGGCAGGA GCTCGGGGAG ACAGCCCTTC ATCTTGCCGT CAGGACCGCA 
GACCAGACAT CTCTCCATCT GGTTGATTTC CTTGTACAAA ACTGTGGGAA CCTGGATAAG 
CAAACGTCTG TGGGGAATAC AGTTCTGCAC TATTGCAGCA TGTACGGCAA GCCGGAGTGT 
CTGAAGCTGC TGCTTAGGAG CAAGCCCACC GTGGACATCG TTAACCAGAA TGGAGAAACT 
GCCTTGGATA TAGCAAAGAG ACTCAAAGCT ACCCAGTGTG AAGATCTGCT TTCCCAGGCT 
AAATCTGGAA AGTTCAACCC TCATGTCCAC GTAGAGTATG AGTGGAATCT TCGACAGGAC 
GAGATGGATG AGAGCGATGA CGATCTGGAT GACAAGCCAA GCCCGATCAA AAAGGAACGC 
TCGCCCAGAC CACAGAGCTT CTGCCACTCC TCCAGCATCT CGCCACAGGA CAAGCTGGCA 
CTGCCAGGGT TCAGCACTCC ACGGGACAAG CAGCGGCTCT CCTACGGAGC CTTCACCAAC 
CAGATCTTCG CCTCTACGAG CACAGATTTG CCCACATCAC CCACCAGTGA GGCTCCCCCT 
TTGCCACCTC GGAACGCCGG GAAAGGTCCA ACTGGCCCAC CTTCAACACT CCCTCTAGGC 
ACCCAGACCT CTAGTGGCAG CTCCACCCTA TCCAAGAAGA GGCCTCCTCC CCCACCACCA 
GGACACAAGA GAACCCTGTC TGACCCTCCC AGCCCACTAC CTCACGGGCC CCCAAACAAA 
GGCGCAATTC CTTGGGGTAA TGATGTGGGC CCATTATCTT CAAGTAAGAC GGCCAACAAG 
TTTGAGGGGC TGTCTCAGCA AGCAAGCACC AGTTCTGCTA AGACTGCCCT TGGCCCGAGA 
GTGCTTCCTA AACTACCTCA GAAAGTGGCA CTAAGGAAGA CGGAGACCAG CCATCATCTC 
TCCCTCGACA GAACCAACAT CCCACCTGAG ACTTTTCAGA AATCATCACA GTTGACAGAG 
TTACCCCAAA AGCCACCACT TGGAGAGCTG CCCCCGAAGC CTGTGGAACT GGCCCCCAAG 
CCCCAAGTTG GAGAGCTGCC ACCTAAGCCT GGAGAGCTAC CCCCTAAGCC CCAATTAGGT 
GACCTGCCCC CCAAGCCACA GCTCTCAGAC TTACCTCCCA AGCCACAGAT GAAGGACCTG 
CCCCCTAAGC CGCAGCTGGG GGATCTGCTG GCAAAGTCCC AGGCTGGCGA TGTCTCAGCC 
AAGGTGCAGC CACCCTCAGA GGTCACACAG AGGTCACACA CCGGGGATCT GTCTCCAAAT 
GTACAGTCCA GAGATGCCAT CCAGAAGCAA GCATCTGAAG ACTCCAACGA CCTCACACCC 
ACGCTGCCAG AGACACCCGT ACCACTGCCG AGAAAAATCA ATACGGGGAA AAATAAAGTG 
AGGCGGGTGA AGACCATTTA TGACTGCCAG GCAGATAATG ACGACGAACT CACATTTATT 
GAGGGGGAAG TGATCATTGT CACCGGGGAA GAGGACCAGG AGTGGTGGAT CGGGCATATC 
GAAGGACAGC CTGAAAGGAA GGGTGTCTTT CCAGTGTCCT TTGTCCACAT CCTGTCTGAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asap1 ( XM_006520400.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006520463.1 Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X9, mRNA.

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CloneID	OMu50921
Clone ID Related Accession (Same CDS sequence)	XM_006520400.4 , XM_006520400.3
Accession Version	XM_006520400.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3456bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006520400.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCAG GTCTGAGTGG GCCACAGGGC TTGCCTTCCA AAGCAGGCTT TAATGGAGGC 
CAGTCATACG CTTTCAAGGG CTCCTGGAGG CCAATCCCAA GGACTAGGAT GCCGGACCAG 
ATCTCCGTGT CCGAGTTCAT CGCCGAGACC ACCGAGGACT ACAACTCGCC CACCACGTCC 
AGCTTCACCA CGCGGCTGCA CAACTGCAGG AACACCGTCA CGCTGCTGGA GGAGGCTCTA 
GACCAAGATA GAACAGCCCT ACAGAAAGTG AAGAAATCTG TAAAAGCAAT ATATAATTCC 
GGTCAAGATC ATGTACAAAA TGAAGAAAAC TATGCACAAG TTCTGGATAA GTTTGGAAGT 
AATTTTTTAA GCAGAGACAA CCCTGACCTT GGCACCGCTT TTGTCAAGTT TTCTACACTC 
ACAAAGGAAC TGTCCACGCT GCTGAAAAAT CTGCTCCAGG GTCTGAGCCA CAATGTGATC 
TTCACCTTGG ATTCCTTGTT AAAAGGAGAC TTGAAGGGGG TCAAAGGAGA TCTCAAGAAG 
CCATTTGACA AAGCCTGGAA AGATTATGAG ACGAAGTTTA CAAAAATTGA GAAGGAGAAA 
AGAGAGCACG CAAAACAGCA CGGGATGATC CGAACAGAGA TAACAGGCGC TGAGATTGCA 
GAAGAAATGG AAAAGGAACG GCGGCTTTTT CAGCTCCAGA TGTGTGAATA TCTCATTAAA 
GTAAATGAAA TCAAGACCAA AAAGGGTGTG GACCTGCTGC AGAACCTTAT AAAGTATTAC 
CATGCACAGT GCAATTTCTT TCAGGATGGC TTGAAAACGG CTGATAAACT GAAACAGTAC 
ATAGAAAAGC TGGCTGCTGA CTTATATAAT ATAAAGCAAA CCCAAGATGA AGAAAAAAAA 
CAGCTAACTG CACTCCGAGA TCTAATAAAA TCCTCTCTGC AACTTGATCC GAAAGAATCT 
AGAAGAGACT CTCAGAGCCG GCAAGGTGGG TACAGCATGC ATCAGCTCCA GGGCAACAAG 
GAGTACGGCA GTGAAAAGAA GGGCTTCCTG CTGAAGAAGA GTGATGGGAT CCGGAAAGTG 
TGGCAGAGAC GGAAGTGTGC GGTCAAGAAC GGGATCCTGA CCATCTCCCA CGCAACTTCC 
AACAGGCAGC CTGCTAAGTT AAACCTCCTC ACCTGCCAAG TGAAACCGAA TGCTGAGGAC 
AAGAAGTCTT TTGACCTGAT ATCACATAAT CGAACATATC ACTTTCAAGC AGAAGATGAG 
CAGGATTATA TAGCGTGGAT ATCAGTACTG ACGAATAGCA AAGAAGAGGC CCTAACCATG 
GCCTTCCGTG GTGAGCAGAG CACAGGGGAG AATAGCCTGG AGGACCTTAC CAAAGCCATC 
ATCGAGGATG TACAGCGGCT CCCTGGGAAT GACATCTGCT GTGACTGCGG CTCATCAGAA 
CCCACGTGGC TTTCAACCAA CTTGGGTATT TTGACCTGTA TAGAATGTTC CGGAATCCAT 
AGGGAAATGG GGGTTCATAT TTCTCGCATT CAGTCTTTGG AACTAGACAA ATTAGGAACT 
TCCGAACTCT TGCTGGCCAA GAATGTAGGA AACAATAGTT TTAATGATAT TATGGAAGCA 
AATTTACCCA GCCCTTCACC AAAGCCCACC CCTTCAAGTG ATATGACCGT GCGTAAAGAG 
TACATCACTG CAAAGTATGT CGACCACCGC TTCTCCAGGA AGACCTGTGC CTCTTCCTCA 
GCAAAGCTGA ACGAATTGCT TGAGGCCATC AAGTCCAGGG ACTTACTTGC ATTAATCCAA 
GTCTATGCAG AGGGGGTGGA GTTAATGGAG CCACTGCTGG AACCTGGGCA GGAGCTCGGG 
GAGACAGCCC TTCATCTTGC CGTCAGGACC GCAGACCAGA CATCTCTCCA TCTGGTTGAT 
TTCCTTGTAC AAAACTGTGG GAACCTGGAT AAGCAAACGT CTGTGGGGAA TACAGTTCTG 
CACTATTGCA GCATGTACGG CAAGCCGGAG TGTCTGAAGC TGCTGCTTAG GAGCAAGCCC 
ACCGTGGACA TCGTTAACCA GAATGGAGAA ACTGCCTTGG ATATAGCAAA GAGACTCAAA 
GCTACCCAGT GTGAAGATCT GCTTTCCCAG GCTAAATCTG GAAAGTTCAA CCCTCATGTC 
CACGTAGAGT ATGAGTGGAA TCTTCGACAG GACGAGATGG ATGAGAGCGA TGACGATCTG 
GATGACAAGC CAAGCCCGAT CAAAAAGGAA CGCTCGCCCA GACCACAGAG CTTCTGCCAC 
TCCTCCAGCA TCTCGCCACA GGACAAGCTG GCACTGCCAG GGTTCAGCAC TCCACGGGAC 
AAGCAGCGGC TCTCCTACGG AGCCTTCACC AACCAGATCT TCGCCTCTAC GAGCACAGAT 
TTGCCCACAT CACCCACCAG TGAGGCTCCC CCTTTGCCAC CTCGGAACGC CGGGAAAGGT 
CCAACTGGCC CACCTTCAAC ACTCCCTCTA GGCACCCAGA CCTCTAGTGG CAGCTCCACC 
CTATCCAAGA AGAGGCCTCC TCCCCCACCA CCAGGACACA AGAGAACCCT GTCTGACCCT 
CCCAGCCCAC TACCTCACGG GCCCCCAAAC AAAGGCGCAA TTCCTTGGGG TAATGATGTG 
GGCCCATTAT CTTCAAGTAA GACGGCCAAC AAGTTTGAGG GGCTGTCTCA GCAAGCAAGC 
ACCAGTTCTG CTAAGACTGC CCTTGGCCCG AGAGTGCTTC CTAAACTACC TCAGAAAGTG 
GCACTAAGGA AGACGGAGAC CAGCCATCAT CTCTCCCTCG ACAGAACCAA CATCCCACCT 
GAGACTTTTC AGAAATCATC ACAGTTGACA GAGTTACCCC AAAAGCCACC ACTTGGAGAG 
CTGCCCCCGA AGCCTGTGGA ACTGGCCCCC AAGCCCCAAG TTGGAGAGCT GCCACCTAAG 
CCTGGAGAGC TACCCCCTAA GCCCCAATTA GGTGACCTGC CCCCCAAGCC ACAGCTCTCA 
GACTTACCTC CCAAGCCACA GATGAAGGAC CTGCCCCCTA AGCCGCAGCT GGGGGATCTG 
CTGGCAAAGT CCCAGGCTGG CGATGTCTCA GCCAAGGTGC AGCCACCCTC AGAGGTCACA 
CAGAGGTCAC ACACCGGGGA TCTGTCTCCA AATGTACAGT CCAGAGATGC CATCCAGAAG 
CAAGCATCTG AAGACTCCAA CGACCTCACA CCCACGCTGC CAGAGACACC CGTACCACTG 
CCGAGAAAAA TCAATACGGG GAAAAATAAA GTGAGGCGGG TGAAGACCAT TTATGACTGC 
CAGGCAGATA ATGACGACGA ACTCACATTT ATTGAGGGGG AAGTGATCAT TGTCACCGGG 
GAAGAGGACC AGGAGTGGTG GATCGGGCAT ATCGAAGGAC AGCCTGAAAG GAAGGGTGTC 
TTTCCAGTGT CCTTTGTCCA CATCCTGTCT GACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006520463.1
CDS	202..3657
Translation	MQAGLSGPQGLPSKAGFNGGQSYAFKGSWRPIPRTRMPDQISVSEFIAETTEDYNSPTTSSFTTRLHNCRNTVTLLEEALDQDRTALQKVKKSVKAIYNSGQDHVQNEENYAQVLDKFGSNFLSRDNPDLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGVKGDLKKPFDKAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDPKESRRDSQSRQGGYSMHQLQGNKEYGSEKKGFLLKKSDGIRKVWQRRKCAVKNGILTISHATSNRQPAKLNLLTCQVKPNAEDKKSFDLISHNRTYHFQAEDEQDYIAWISVLTNSKEEALTMAFRGEQSTGENSLEDLTKAIIEDVQRLPGNDICCDCGSSEPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDKLGTSELLLAKNVGNNSFNDIMEANLPSPSPKPTPSSDMTVRKEYITAKYVDHRFSRKTCASSSAKLNELLEAIKSRDLLALIQVYAEGVELMEPLLEPGQELGETALHLAVRTADQTSLHLVDFLVQNCGNLDKQTSVGNTVLHYCSMYGKPECLKLLLRSKPTVDIVNQNGETALDIAKRLKATQCEDLLSQAKSGKFNPHVHVEYEWNLRQDEMDESDDDLDDKPSPIKKERSPRPQSFCHSSSISPQDKLALPGFSTPRDKQRLSYGAFTNQIFASTSTDLPTSPTSEAPPLPPRNAGKGPTGPPSTLPLGTQTSSGSSTLSKKRPPPPPPGHKRTLSDPPSPLPHGPPNKGAIPWGNDVGPLSSSKTANKFEGLSQQASTSSAKTALGPRVLPKLPQKVALRKTETSHHLSLDRTNIPPETFQKSSQLTELPQKPPLGELPPKPVELAPKPQVGELPPKPGELPPKPQLGDLPPKPQLSDLPPKPQMKDLPPKPQLGDLLAKSQAGDVSAKVQPPSEVTQRSHTGDLSPNVQSRDAIQKQASEDSNDLTPTLPETPVPLPRKINTGKNKVRRVKTIYDCQADNDDELTFIEGEVIIVTGEEDQEWWIGHIEGQPERKGVFPVSFVHILSD

Target ORF information:

RefSeq Version	XM_006520400.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006520400.4

ORF Insert Sequence:

ATGCAGGCAG GTCTGAGTGG GCCACAGGGC TTGCCTTCCA AAGCAGGCTT TAATGGAGGC 
CAGTCATACG CTTTCAAGGG CTCCTGGAGG CCAATCCCAA GGACTAGGAT GCCGGACCAG 
ATCTCCGTGT CCGAGTTCAT CGCCGAGACC ACCGAGGACT ACAACTCGCC CACCACGTCC 
AGCTTCACCA CGCGGCTGCA CAACTGCAGG AACACCGTCA CGCTGCTGGA GGAGGCTCTA 
GACCAAGATA GAACAGCCCT ACAGAAAGTG AAGAAATCTG TAAAAGCAAT ATATAATTCC 
GGTCAAGATC ATGTACAAAA TGAAGAAAAC TATGCACAAG TTCTGGATAA GTTTGGAAGT 
AATTTTTTAA GCAGAGACAA CCCTGACCTT GGCACCGCTT TTGTCAAGTT TTCTACACTC 
ACAAAGGAAC TGTCCACGCT GCTGAAAAAT CTGCTCCAGG GTCTGAGCCA CAATGTGATC 
TTCACCTTGG ATTCCTTGTT AAAAGGAGAC TTGAAGGGGG TCAAAGGAGA TCTCAAGAAG 
CCATTTGACA AAGCCTGGAA AGATTATGAG ACGAAGTTTA CAAAAATTGA GAAGGAGAAA 
AGAGAGCACG CAAAACAGCA CGGGATGATC CGAACAGAGA TAACAGGCGC TGAGATTGCA 
GAAGAAATGG AAAAGGAACG GCGGCTTTTT CAGCTCCAGA TGTGTGAATA TCTCATTAAA 
GTAAATGAAA TCAAGACCAA AAAGGGTGTG GACCTGCTGC AGAACCTTAT AAAGTATTAC 
CATGCACAGT GCAATTTCTT TCAGGATGGC TTGAAAACGG CTGATAAACT GAAACAGTAC 
ATAGAAAAGC TGGCTGCTGA CTTATATAAT ATAAAGCAAA CCCAAGATGA AGAAAAAAAA 
CAGCTAACTG CACTCCGAGA TCTAATAAAA TCCTCTCTGC AACTTGATCC GAAAGAATCT 
AGAAGAGACT CTCAGAGCCG GCAAGGTGGG TACAGCATGC ATCAGCTCCA GGGCAACAAG 
GAGTACGGCA GTGAAAAGAA GGGCTTCCTG CTGAAGAAGA GTGATGGGAT CCGGAAAGTG 
TGGCAGAGAC GGAAGTGTGC GGTCAAGAAC GGGATCCTGA CCATCTCCCA CGCAACTTCC 
AACAGGCAGC CTGCTAAGTT AAACCTCCTC ACCTGCCAAG TGAAACCGAA TGCTGAGGAC 
AAGAAGTCTT TTGACCTGAT ATCACATAAT CGAACATATC ACTTTCAAGC AGAAGATGAG 
CAGGATTATA TAGCGTGGAT ATCAGTACTG ACGAATAGCA AAGAAGAGGC CCTAACCATG 
GCCTTCCGTG GTGAGCAGAG CACAGGGGAG AATAGCCTGG AGGACCTTAC CAAAGCCATC 
ATCGAGGATG TACAGCGGCT CCCTGGGAAT GACATCTGCT GTGACTGCGG CTCATCAGAA 
CCCACGTGGC TTTCAACCAA CTTGGGTATT TTGACCTGTA TAGAATGTTC CGGAATCCAT 
AGGGAAATGG GGGTTCATAT TTCTCGCATT CAGTCTTTGG AACTAGACAA ATTAGGAACT 
TCCGAACTCT TGCTGGCCAA GAATGTAGGA AACAATAGTT TTAATGATAT TATGGAAGCA 
AATTTACCCA GCCCTTCACC AAAGCCCACC CCTTCAAGTG ATATGACCGT GCGTAAAGAG 
TACATCACTG CAAAGTATGT CGACCACCGC TTCTCCAGGA AGACCTGTGC CTCTTCCTCA 
GCAAAGCTGA ACGAATTGCT TGAGGCCATC AAGTCCAGGG ACTTACTTGC ATTAATCCAA 
GTCTATGCAG AGGGGGTGGA GTTAATGGAG CCACTGCTGG AACCTGGGCA GGAGCTCGGG 
GAGACAGCCC TTCATCTTGC CGTCAGGACC GCAGACCAGA CATCTCTCCA TCTGGTTGAT 
TTCCTTGTAC AAAACTGTGG GAACCTGGAT AAGCAAACGT CTGTGGGGAA TACAGTTCTG 
CACTATTGCA GCATGTACGG CAAGCCGGAG TGTCTGAAGC TGCTGCTTAG GAGCAAGCCC 
ACCGTGGACA TCGTTAACCA GAATGGAGAA ACTGCCTTGG ATATAGCAAA GAGACTCAAA 
GCTACCCAGT GTGAAGATCT GCTTTCCCAG GCTAAATCTG GAAAGTTCAA CCCTCATGTC 
CACGTAGAGT ATGAGTGGAA TCTTCGACAG GACGAGATGG ATGAGAGCGA TGACGATCTG 
GATGACAAGC CAAGCCCGAT CAAAAAGGAA CGCTCGCCCA GACCACAGAG CTTCTGCCAC 
TCCTCCAGCA TCTCGCCACA GGACAAGCTG GCACTGCCAG GGTTCAGCAC TCCACGGGAC 
AAGCAGCGGC TCTCCTACGG AGCCTTCACC AACCAGATCT TCGCCTCTAC GAGCACAGAT 
TTGCCCACAT CACCCACCAG TGAGGCTCCC CCTTTGCCAC CTCGGAACGC CGGGAAAGGT 
CCAACTGGCC CACCTTCAAC ACTCCCTCTA GGCACCCAGA CCTCTAGTGG CAGCTCCACC 
CTATCCAAGA AGAGGCCTCC TCCCCCACCA CCAGGACACA AGAGAACCCT GTCTGACCCT 
CCCAGCCCAC TACCTCACGG GCCCCCAAAC AAAGGCGCAA TTCCTTGGGG TAATGATGTG 
GGCCCATTAT CTTCAAGTAA GACGGCCAAC AAGTTTGAGG GGCTGTCTCA GCAAGCAAGC 
ACCAGTTCTG CTAAGACTGC CCTTGGCCCG AGAGTGCTTC CTAAACTACC TCAGAAAGTG 
GCACTAAGGA AGACGGAGAC CAGCCATCAT CTCTCCCTCG ACAGAACCAA CATCCCACCT 
GAGACTTTTC AGAAATCATC ACAGTTGACA GAGTTACCCC AAAAGCCACC ACTTGGAGAG 
CTGCCCCCGA AGCCTGTGGA ACTGGCCCCC AAGCCCCAAG TTGGAGAGCT GCCACCTAAG 
CCTGGAGAGC TACCCCCTAA GCCCCAATTA GGTGACCTGC CCCCCAAGCC ACAGCTCTCA 
GACTTACCTC CCAAGCCACA GATGAAGGAC CTGCCCCCTA AGCCGCAGCT GGGGGATCTG 
CTGGCAAAGT CCCAGGCTGG CGATGTCTCA GCCAAGGTGC AGCCACCCTC AGAGGTCACA 
CAGAGGTCAC ACACCGGGGA TCTGTCTCCA AATGTACAGT CCAGAGATGC CATCCAGAAG 
CAAGCATCTG AAGACTCCAA CGACCTCACA CCCACGCTGC CAGAGACACC CGTACCACTG 
CCGAGAAAAA TCAATACGGG GAAAAATAAA GTGAGGCGGG TGAAGACCAT TTATGACTGC 
CAGGCAGATA ATGACGACGA ACTCACATTT ATTGAGGGGG AAGTGATCAT TGTCACCGGG 
GAAGAGGACC AGGAGTGGTG GATCGGGCAT ATCGAAGGAC AGCCTGAAAG GAAGGGTGTC 
TTTCCAGTGT CCTTTGTCCA CATCCTGTCT GACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asap1 ( XM_006520401.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006520464.1 Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X10, mRNA.

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CloneID	OMu50922
Clone ID Related Accession (Same CDS sequence)	XM_006520401.3 , XM_006520401.4
Accession Version	XM_006520401.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3447bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006520401.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCAG GTCTGAGTGG GCCACAGGGC TTGCCTTCCA AAGCAGGCTT TAATGGAGGC 
CAGTCATACG CTTTCAAGGG CTCCTGGAGG CCAATCCCAA GGACTAGGAT GCCGGACCAG 
ATCTCCGTGT CCGAGTTCAT CGCCGAGACC ACCGAGGACT ACAACTCGCC CACCACGTCC 
AGCTTCACCA CGCGGCTGCA CAACTGCAGG AACACCGTCA CGCTGCTGGA GGAGGCTCTA 
GACCAAGATA GAACAGCCCT ACAGAAAGTG AAGAAATCTG TAAAAGCAAT ATATAATTCC 
GGTCAAGATC ATGTACAAAA TGAAGAAAAC TATGCACAAG TTCTGGATAA GTTTGGAAGT 
AATTTTTTAA GCAGAGACAA CCCTGACCTT GGCACCGCTT TTGTCAAGTT TTCTACACTC 
ACAAAGGAAC TGTCCACGCT GCTGAAAAAT CTGCTCCAGG GTCTGAGCCA CAATGTGATC 
TTCACCTTGG ATTCCTTGTT AAAAGGAGAC TTGAAGGGGG TCAAAGGAGA TCTCAAGAAG 
CCATTTGACA AAGCCTGGAA AGATTATGAG ACGAAGTTTA CAAAAATTGA GAAGGAGAAA 
AGAGAGCACG CAAAACAGCA CGGGATGATC CGAACAGAGA TAACAGGCGC TGAGATTGCA 
GAAGAAATGG AAAAGGAACG GCGGCTTTTT CAGCTCCAGA TGTGTGAATA TCTCATTAAA 
GTAAATGAAA TCAAGACCAA AAAGGGTGTG GACCTGCTGC AGAACCTTAT AAAGTATTAC 
CATGCACAGT GCAATTTCTT TCAGGATGGC TTGAAAACGG CTGATAAACT GAAACAGTAC 
ATAGAAAAGC TGGCTGCTGA CTTATATAAT ATAAAGCAAA CCCAAGATGA AGAAAAAAAA 
CAGCTAACTG CACTCCGAGA TCTAATAAAA TCCTCTCTGC AACTTGATCC GAAAGAAGAC 
TCTCAGAGCC GGCAAGGTGG GTACAGCATG CATCAGCTCC AGGGCAACAA GGAGTACGGC 
AGTGAAAAGA AGGGCTTCCT GCTGAAGAAG AGTGATGGGA TCCGGAAAGT GTGGCAGAGA 
CGGAAGTGTG CGGTCAAGAA CGGGATCCTG ACCATCTCCC ACGCAACTTC CAACAGGCAG 
CCTGCTAAGT TAAACCTCCT CACCTGCCAA GTGAAACCGA ATGCTGAGGA CAAGAAGTCT 
TTTGACCTGA TATCACATAA TCGAACATAT CACTTTCAAG CAGAAGATGA GCAGGATTAT 
ATAGCGTGGA TATCAGTACT GACGAATAGC AAAGAAGAGG CCCTAACCAT GGCCTTCCGT 
GGTGAGCAGA GCACAGGGGA GAATAGCCTG GAGGACCTTA CCAAAGCCAT CATCGAGGAT 
GTACAGCGGC TCCCTGGGAA TGACATCTGC TGTGACTGCG GCTCATCAGA ACCCACGTGG 
CTTTCAACCA ACTTGGGTAT TTTGACCTGT ATAGAATGTT CCGGAATCCA TAGGGAAATG 
GGGGTTCATA TTTCTCGCAT TCAGTCTTTG GAACTAGACA AATTAGGAAC TTCCGAACTC 
TTGCTGGCCA AGAATGTAGG AAACAATAGT TTTAATGATA TTATGGAAGC AAATTTACCC 
AGCCCTTCAC CAAAGCCCAC CCCTTCAAGT GATATGACCG TGCGTAAAGA GTACATCACT 
GCAAAGTATG TCGACCACCG CTTCTCCAGG AAGACCTGTG CCTCTTCCTC AGCAAAGCTG 
AACGAATTGC TTGAGGCCAT CAAGTCCAGG GACTTACTTG CATTAATCCA AGTCTATGCA 
GAGGGGGTGG AGTTAATGGA GCCACTGCTG GAACCTGGGC AGGAGCTCGG GGAGACAGCC 
CTTCATCTTG CCGTCAGGAC CGCAGACCAG ACATCTCTCC ATCTGGTTGA TTTCCTTGTA 
CAAAACTGTG GGAACCTGGA TAAGCAAACG TCTGTGGGGA ATACAGTTCT GCACTATTGC 
AGCATGTACG GCAAGCCGGA GTGTCTGAAG CTGCTGCTTA GGAGCAAGCC CACCGTGGAC 
ATCGTTAACC AGAATGGAGA AACTGCCTTG GATATAGCAA AGAGACTCAA AGCTACCCAG 
TGTGAAGATC TGCTTTCCCA GGCTAAATCT GGAAAGTTCA ACCCTCATGT CCACGTAGAG 
TATGAGTGGA ATCTTCGACA GGACGAGATG GATGAGAGCG ATGACGATCT GGATGACAAG 
CCAAGCCCGA TCAAAAAGGA ACGCTCGCCC AGACCACAGA GCTTCTGCCA CTCCTCCAGC 
ATCTCGCCAC AGGACAAGCT GGCACTGCCA GGGTTCAGCA CTCCACGGGA CAAGCAGCGG 
CTCTCCTACG GAGCCTTCAC CAACCAGATC TTCGCCTCTA CGAGCACAGA TTTGCCCACA 
TCACCCACCA GTGAGGCTCC CCCTTTGCCA CCTCGGAACG CCGGGAAAGG TCCAACTGGC 
CCACCTTCAA CACTCCCTCT AGGCACCCAG ACCTCTAGTG GCAGCTCCAC CCTATCCAAG 
AAGAGGCCTC CTCCCCCACC ACCAGGACAC AAGAGAACCC TGTCTGACCC TCCCAGCCCA 
CTACCTCACG GGCCCCCAAA CAAAGGCGCA ATTCCTTGGG GTAATGATGT GGGCCCATTA 
TCTTCAAGTA AGACGGCCAA CAAGTTTGAG GGGCTGTCTC AGCAAGCAAG CACCAGTTCT 
GCTAAGACTG CCCTTGGCCC GAGAGTGCTT CCTAAACTAC CTCAGAAAGT GGCACTAAGG 
AAGACGGAGA CCAGCCATCA TCTCTCCCTC GACAGAACCA ACATCCCACC TGAGACTTTT 
CAGAAATCAT CACAGTTGAC AGAGTTACCC CAAAAGCCAC CACTTGGAGA GCTGCCCCCG 
AAGCCTGTGG AACTGGCCCC CAAGCCCCAA GTTGGAGAGC TGCCACCTAA GCCTGGAGAG 
CTACCCCCTA AGCCCCAATT AGGTGACCTG CCCCCCAAGC CACAGCTCTC AGACTTACCT 
CCCAAGCCAC AGATGAAGGA CCTGCCCCCT AAGCCGCAGC TGGGGGATCT GCTGGCAAAG 
TCCCAGGCTG GCGATGTCTC AGCCAAGGTG CAGCCACCCT CAGAGGTCAC ACAGAGGTCA 
CACACCGGGG ATCTGTCTCC AAATGTACAG TCCAGAGATG CCATCCAGAA GCAAGCATCT 
GAAGACTCCA ACGACCTCAC ACCCACGCTG CCAGAGACAC CCGTACCACT GCCGAGAAAA 
ATCAATACGG GGAAAAATAA AGTGAGGCGG GTGAAGACCA TTTATGACTG CCAGGCAGAT 
AATGACGACG AACTCACATT TATTGAGGGG GAAGTGATCA TTGTCACCGG GGAAGAGGAC 
CAGGAGTGGT GGATCGGGCA TATCGAAGGA CAGCCTGAAA GGAAGGGTGT CTTTCCAGTG 
TCCTTTGTCC ACATCCTGTC TGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006520464.1
CDS	202..3648
Translation	MQAGLSGPQGLPSKAGFNGGQSYAFKGSWRPIPRTRMPDQISVSEFIAETTEDYNSPTTSSFTTRLHNCRNTVTLLEEALDQDRTALQKVKKSVKAIYNSGQDHVQNEENYAQVLDKFGSNFLSRDNPDLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGVKGDLKKPFDKAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDPKEDSQSRQGGYSMHQLQGNKEYGSEKKGFLLKKSDGIRKVWQRRKCAVKNGILTISHATSNRQPAKLNLLTCQVKPNAEDKKSFDLISHNRTYHFQAEDEQDYIAWISVLTNSKEEALTMAFRGEQSTGENSLEDLTKAIIEDVQRLPGNDICCDCGSSEPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDKLGTSELLLAKNVGNNSFNDIMEANLPSPSPKPTPSSDMTVRKEYITAKYVDHRFSRKTCASSSAKLNELLEAIKSRDLLALIQVYAEGVELMEPLLEPGQELGETALHLAVRTADQTSLHLVDFLVQNCGNLDKQTSVGNTVLHYCSMYGKPECLKLLLRSKPTVDIVNQNGETALDIAKRLKATQCEDLLSQAKSGKFNPHVHVEYEWNLRQDEMDESDDDLDDKPSPIKKERSPRPQSFCHSSSISPQDKLALPGFSTPRDKQRLSYGAFTNQIFASTSTDLPTSPTSEAPPLPPRNAGKGPTGPPSTLPLGTQTSSGSSTLSKKRPPPPPPGHKRTLSDPPSPLPHGPPNKGAIPWGNDVGPLSSSKTANKFEGLSQQASTSSAKTALGPRVLPKLPQKVALRKTETSHHLSLDRTNIPPETFQKSSQLTELPQKPPLGELPPKPVELAPKPQVGELPPKPGELPPKPQLGDLPPKPQLSDLPPKPQMKDLPPKPQLGDLLAKSQAGDVSAKVQPPSEVTQRSHTGDLSPNVQSRDAIQKQASEDSNDLTPTLPETPVPLPRKINTGKNKVRRVKTIYDCQADNDDELTFIEGEVIIVTGEEDQEWWIGHIEGQPERKGVFPVSFVHILSD

Target ORF information:

RefSeq Version	XM_006520401.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006520401.4

ORF Insert Sequence:

ATGCAGGCAG GTCTGAGTGG GCCACAGGGC TTGCCTTCCA AAGCAGGCTT TAATGGAGGC 
CAGTCATACG CTTTCAAGGG CTCCTGGAGG CCAATCCCAA GGACTAGGAT GCCGGACCAG 
ATCTCCGTGT CCGAGTTCAT CGCCGAGACC ACCGAGGACT ACAACTCGCC CACCACGTCC 
AGCTTCACCA CGCGGCTGCA CAACTGCAGG AACACCGTCA CGCTGCTGGA GGAGGCTCTA 
GACCAAGATA GAACAGCCCT ACAGAAAGTG AAGAAATCTG TAAAAGCAAT ATATAATTCC 
GGTCAAGATC ATGTACAAAA TGAAGAAAAC TATGCACAAG TTCTGGATAA GTTTGGAAGT 
AATTTTTTAA GCAGAGACAA CCCTGACCTT GGCACCGCTT TTGTCAAGTT TTCTACACTC 
ACAAAGGAAC TGTCCACGCT GCTGAAAAAT CTGCTCCAGG GTCTGAGCCA CAATGTGATC 
TTCACCTTGG ATTCCTTGTT AAAAGGAGAC TTGAAGGGGG TCAAAGGAGA TCTCAAGAAG 
CCATTTGACA AAGCCTGGAA AGATTATGAG ACGAAGTTTA CAAAAATTGA GAAGGAGAAA 
AGAGAGCACG CAAAACAGCA CGGGATGATC CGAACAGAGA TAACAGGCGC TGAGATTGCA 
GAAGAAATGG AAAAGGAACG GCGGCTTTTT CAGCTCCAGA TGTGTGAATA TCTCATTAAA 
GTAAATGAAA TCAAGACCAA AAAGGGTGTG GACCTGCTGC AGAACCTTAT AAAGTATTAC 
CATGCACAGT GCAATTTCTT TCAGGATGGC TTGAAAACGG CTGATAAACT GAAACAGTAC 
ATAGAAAAGC TGGCTGCTGA CTTATATAAT ATAAAGCAAA CCCAAGATGA AGAAAAAAAA 
CAGCTAACTG CACTCCGAGA TCTAATAAAA TCCTCTCTGC AACTTGATCC GAAAGAAGAC 
TCTCAGAGCC GGCAAGGTGG GTACAGCATG CATCAGCTCC AGGGCAACAA GGAGTACGGC 
AGTGAAAAGA AGGGCTTCCT GCTGAAGAAG AGTGATGGGA TCCGGAAAGT GTGGCAGAGA 
CGGAAGTGTG CGGTCAAGAA CGGGATCCTG ACCATCTCCC ACGCAACTTC CAACAGGCAG 
CCTGCTAAGT TAAACCTCCT CACCTGCCAA GTGAAACCGA ATGCTGAGGA CAAGAAGTCT 
TTTGACCTGA TATCACATAA TCGAACATAT CACTTTCAAG CAGAAGATGA GCAGGATTAT 
ATAGCGTGGA TATCAGTACT GACGAATAGC AAAGAAGAGG CCCTAACCAT GGCCTTCCGT 
GGTGAGCAGA GCACAGGGGA GAATAGCCTG GAGGACCTTA CCAAAGCCAT CATCGAGGAT 
GTACAGCGGC TCCCTGGGAA TGACATCTGC TGTGACTGCG GCTCATCAGA ACCCACGTGG 
CTTTCAACCA ACTTGGGTAT TTTGACCTGT ATAGAATGTT CCGGAATCCA TAGGGAAATG 
GGGGTTCATA TTTCTCGCAT TCAGTCTTTG GAACTAGACA AATTAGGAAC TTCCGAACTC 
TTGCTGGCCA AGAATGTAGG AAACAATAGT TTTAATGATA TTATGGAAGC AAATTTACCC 
AGCCCTTCAC CAAAGCCCAC CCCTTCAAGT GATATGACCG TGCGTAAAGA GTACATCACT 
GCAAAGTATG TCGACCACCG CTTCTCCAGG AAGACCTGTG CCTCTTCCTC AGCAAAGCTG 
AACGAATTGC TTGAGGCCAT CAAGTCCAGG GACTTACTTG CATTAATCCA AGTCTATGCA 
GAGGGGGTGG AGTTAATGGA GCCACTGCTG GAACCTGGGC AGGAGCTCGG GGAGACAGCC 
CTTCATCTTG CCGTCAGGAC CGCAGACCAG ACATCTCTCC ATCTGGTTGA TTTCCTTGTA 
CAAAACTGTG GGAACCTGGA TAAGCAAACG TCTGTGGGGA ATACAGTTCT GCACTATTGC 
AGCATGTACG GCAAGCCGGA GTGTCTGAAG CTGCTGCTTA GGAGCAAGCC CACCGTGGAC 
ATCGTTAACC AGAATGGAGA AACTGCCTTG GATATAGCAA AGAGACTCAA AGCTACCCAG 
TGTGAAGATC TGCTTTCCCA GGCTAAATCT GGAAAGTTCA ACCCTCATGT CCACGTAGAG 
TATGAGTGGA ATCTTCGACA GGACGAGATG GATGAGAGCG ATGACGATCT GGATGACAAG 
CCAAGCCCGA TCAAAAAGGA ACGCTCGCCC AGACCACAGA GCTTCTGCCA CTCCTCCAGC 
ATCTCGCCAC AGGACAAGCT GGCACTGCCA GGGTTCAGCA CTCCACGGGA CAAGCAGCGG 
CTCTCCTACG GAGCCTTCAC CAACCAGATC TTCGCCTCTA CGAGCACAGA TTTGCCCACA 
TCACCCACCA GTGAGGCTCC CCCTTTGCCA CCTCGGAACG CCGGGAAAGG TCCAACTGGC 
CCACCTTCAA CACTCCCTCT AGGCACCCAG ACCTCTAGTG GCAGCTCCAC CCTATCCAAG 
AAGAGGCCTC CTCCCCCACC ACCAGGACAC AAGAGAACCC TGTCTGACCC TCCCAGCCCA 
CTACCTCACG GGCCCCCAAA CAAAGGCGCA ATTCCTTGGG GTAATGATGT GGGCCCATTA 
TCTTCAAGTA AGACGGCCAA CAAGTTTGAG GGGCTGTCTC AGCAAGCAAG CACCAGTTCT 
GCTAAGACTG CCCTTGGCCC GAGAGTGCTT CCTAAACTAC CTCAGAAAGT GGCACTAAGG 
AAGACGGAGA CCAGCCATCA TCTCTCCCTC GACAGAACCA ACATCCCACC TGAGACTTTT 
CAGAAATCAT CACAGTTGAC AGAGTTACCC CAAAAGCCAC CACTTGGAGA GCTGCCCCCG 
AAGCCTGTGG AACTGGCCCC CAAGCCCCAA GTTGGAGAGC TGCCACCTAA GCCTGGAGAG 
CTACCCCCTA AGCCCCAATT AGGTGACCTG CCCCCCAAGC CACAGCTCTC AGACTTACCT 
CCCAAGCCAC AGATGAAGGA CCTGCCCCCT AAGCCGCAGC TGGGGGATCT GCTGGCAAAG 
TCCCAGGCTG GCGATGTCTC AGCCAAGGTG CAGCCACCCT CAGAGGTCAC ACAGAGGTCA 
CACACCGGGG ATCTGTCTCC AAATGTACAG TCCAGAGATG CCATCCAGAA GCAAGCATCT 
GAAGACTCCA ACGACCTCAC ACCCACGCTG CCAGAGACAC CCGTACCACT GCCGAGAAAA 
ATCAATACGG GGAAAAATAA AGTGAGGCGG GTGAAGACCA TTTATGACTG CCAGGCAGAT 
AATGACGACG AACTCACATT TATTGAGGGG GAAGTGATCA TTGTCACCGG GGAAGAGGAC 
CAGGAGTGGT GGATCGGGCA TATCGAAGGA CAGCCTGAAA GGAAGGGTGT CTTTCCAGTG 
TCCTTTGTCC ACATCCTGTC TGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asap1 ( XM_006520402.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006520465.1 Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OMu50923
Clone ID Related Accession (Same CDS sequence)	XM_006520402.3 , XM_006520402.4
Accession Version	XM_006520402.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3321bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006520402.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCAG GTCTGAGTGG GCCACAGGGC TTGCCTTCCA AAGCAGGCTT TAATGGAGGC 
CAGTCATACG CTTTCAAGGG CTCCTGGAGG CCAATCCCAA GGACTAGGAT GCCGGACCAG 
ATCTCCGTGT CCGAGTTCAT CGCCGAGACC ACCGAGGACT ACAACTCGCC CACCACGTCC 
AGCTTCACCA CGCGGCTGCA CAACTGCAGG AACACCGTCA CGCTGCTGGA GGAGGCTCTA 
GACCAAGATA GAACAGCCCT ACAGAAAGTG AAGAAATCTG TAAAAGCAAT ATATAATTCC 
GGTCAAGATC ATGTACAAAA TGAAGAAAAC TATGCACAAG TTCTGGATAA GTTTGGAAGT 
AATTTTTTAA GCAGAGACAA CCCTGACCTT GGCACCGCTT TTGTCAAGTT TTCTACACTC 
ACAAAGGAAC TGTCCACGCT GCTGAAAAAT CTGCTCCAGG GTCTGAGCCA CAATGTGATC 
TTCACCTTGG ATTCCTTGTT AAAAGGAGAC TTGAAGGGGG TCAAAGGAGA TCTCAAGAAG 
CCATTTGACA AAGCCTGGAA AGATTATGAG ACGAAGTTTA CAAAAATTGA GAAGGAGAAA 
AGAGAGCACG CAAAACAGCA CGGGATGATC CGAACAGAGA TAACAGGCGC TGAGATTGCA 
GAAGAAATGG AAAAGGAACG GCGGCTTTTT CAGCTCCAGA TGTGTGAATA TCTCATTAAA 
GTAAATGAAA TCAAGACCAA AAAGGGTGTG GACCTGCTGC AGAACCTTAT AAAGTATTAC 
CATGCACAGT GCAATTTCTT TCAGGATGGC TTGAAAACGG CTGATAAACT GAAACAGTAC 
ATAGAAAAGC TGGCTGCTGA CTTATATAAT ATAAAGCAAA CCCAAGATGA AGAAAAAAAA 
CAGCTAACTG CACTCCGAGA TCTAATAAAA TCCTCTCTGC AACTTGATCC GAAAGAAGTA 
GGTGGTTTAT ATGTTGCCAG CAGGGCTAAC AGTTCTAGAA GAGACTCTCA GAGCCGGCAA 
GGTGGGTACA GCATGCATCA GCTCCAGGGC AACAAGGAGT ACGGCAGTGA AAAGAAGGGC 
TTCCTGCTGA AGAAGAGTGA TGGGATCCGG AAAGTGTGGC AGAGACGGAA GTGTGCGGTC 
AAGAACGGGA TCCTGACCAT CTCCCACGCA ACTTCCAACA GGCAGCCTGC TAAGTTAAAC 
CTCCTCACCT GCCAAGTGAA ACCGAATGCT GAGGACAAGA AGTCTTTTGA CCTGATATCA 
CATAATCGAA CATATCACTT TCAAGCAGAA GATGAGCAGG ATTATATAGC GTGGATATCA 
GTACTGACGA ATAGCAAAGA AGAGGCCCTA ACCATGGCCT TCCGTGGTGA GCAGAGCACA 
GGGGAGAATA GCCTGGAGGA CCTTACCAAA GCCATCATCG AGGATGTACA GCGGCTCCCT 
GGGAATGACA TCTGCTGTGA CTGCGGCTCA TCAGAACCCA CGTGGCTTTC AACCAACTTG 
GGTATTTTGA CCTGTATAGA ATGTTCCGGA ATCCATAGGG AAATGGGGGT TCATATTTCT 
CGCATTCAGT CTTTGGAACT AGACAAATTA GGAACTTCCG AACTCTTGCT GGCCAAGAAT 
GTAGGAAACA ATAGTTTTAA TGATATTATG GAAGCAAATT TACCCAGCCC TTCACCAAAG 
CCCACCCCTT CAAGTGATAT GACCGTGCGT AAAGAGTACA TCACTGCAAA GTATGTCGAC 
CACCGCTTCT CCAGGAAGAC CTGTGCCTCT TCCTCAGCAA AGCTGAACGA ATTGCTTGAG 
GCCATCAAGT CCAGGGACTT ACTTGCATTA ATCCAAGTCT ATGCAGAGGG GGTGGAGTTA 
ATGGAGCCAC TGCTGGAACC TGGGCAGGAG CTCGGGGAGA CAGCCCTTCA TCTTGCCGTC 
AGGACCGCAG ACCAGACATC TCTCCATCTG GTTGATTTCC TTGTACAAAA CTGTGGGAAC 
CTGGATAAGC AAACGTCTGT GGGGAATACA GTTCTGCACT ATTGCAGCAT GTACGGCAAG 
CCGGAGTGTC TGAAGCTGCT GCTTAGGAGC AAGCCCACCG TGGACATCGT TAACCAGAAT 
GGAGAAACTG CCTTGGATAT AGCAAAGAGA CTCAAAGCTA CCCAGTGTGA AGATCTGCTT 
TCCCAGGCTA AATCTGGAAA GTTCAACCCT CATGTCCACG TAGAGTATGA GTGGAATCTT 
CGACAGGACG AGATGGATGA GAGCGATGAC GATCTGGATG ACAAGCCAAG CCCGATCAAA 
AAGGAACGCT CGCCCAGACC ACAGAGCTTC TGCCACTCCT CCAGCATCTC GCCACAGGAC 
AAGCTGGCAC TGCCAGGGTT CAGCACTCCA CGGGACAAGC AGCGGCTCTC CTACGGAGCC 
TTCACCAACC AGATCTTCGC CTCTACGAGC ACAGATTTGC CCACATCACC CACCAGTGAG 
GCTCCCCCTT TGCCACCTCG GAACGCCGGG AAAGGTAATG ATGTGGGCCC ATTATCTTCA 
AGTAAGACGG CCAACAAGTT TGAGGGGCTG TCTCAGCAAG CAAGCACCAG TTCTGCTAAG 
ACTGCCCTTG GCCCGAGAGT GCTTCCTAAA CTACCTCAGA AAGTGGCACT AAGGAAGACG 
GAGACCAGCC ATCATCTCTC CCTCGACAGA ACCAACATCC CACCTGAGAC TTTTCAGAAA 
TCATCACAGT TGACAGAGTT ACCCCAAAAG CCACCACTTG GAGAGCTGCC CCCGAAGCCT 
GTGGAACTGG CCCCCAAGCC CCAAGTTGGA GAGCTGCCAC CTAAGCCTGG AGAGCTACCC 
CCTAAGCCCC AATTAGGTGA CCTGCCCCCC AAGCCACAGC TCTCAGACTT ACCTCCCAAG 
CCACAGATGA AGGACCTGCC CCCTAAGCCG CAGCTGGGGG ATCTGCTGGC AAAGTCCCAG 
GCTGGCGATG TCTCAGCCAA GGTGCAGCCA CCCTCAGAGG TCACACAGAG GTCACACACC 
GGGGATCTGT CTCCAAATGT ACAGTCCAGA GATGCCATCC AGAAGCAAGC ATCTGAAGAC 
TCCAACGACC TCACACCCAC GCTGCCAGAG ACACCCGTAC CACTGCCGAG AAAAATCAAT 
ACGGGGAAAA ATAAAGTGAG GCGGGTGAAG ACCATTTATG ACTGCCAGGC AGATAATGAC 
GACGAACTCA CATTTATTGA GGGGGAAGTG ATCATTGTCA CCGGGGAAGA GGACCAGGAG 
TGGTGGATCG GGCATATCGA AGGACAGCCT GAAAGGAAGG GTGTCTTTCC AGTGTCCTTT 
GTCCACATCC TGTCTGACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006520465.1
CDS	202..3522
Translation	MQAGLSGPQGLPSKAGFNGGQSYAFKGSWRPIPRTRMPDQISVSEFIAETTEDYNSPTTSSFTTRLHNCRNTVTLLEEALDQDRTALQKVKKSVKAIYNSGQDHVQNEENYAQVLDKFGSNFLSRDNPDLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGVKGDLKKPFDKAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDPKEVGGLYVASRANSSRRDSQSRQGGYSMHQLQGNKEYGSEKKGFLLKKSDGIRKVWQRRKCAVKNGILTISHATSNRQPAKLNLLTCQVKPNAEDKKSFDLISHNRTYHFQAEDEQDYIAWISVLTNSKEEALTMAFRGEQSTGENSLEDLTKAIIEDVQRLPGNDICCDCGSSEPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDKLGTSELLLAKNVGNNSFNDIMEANLPSPSPKPTPSSDMTVRKEYITAKYVDHRFSRKTCASSSAKLNELLEAIKSRDLLALIQVYAEGVELMEPLLEPGQELGETALHLAVRTADQTSLHLVDFLVQNCGNLDKQTSVGNTVLHYCSMYGKPECLKLLLRSKPTVDIVNQNGETALDIAKRLKATQCEDLLSQAKSGKFNPHVHVEYEWNLRQDEMDESDDDLDDKPSPIKKERSPRPQSFCHSSSISPQDKLALPGFSTPRDKQRLSYGAFTNQIFASTSTDLPTSPTSEAPPLPPRNAGKGNDVGPLSSSKTANKFEGLSQQASTSSAKTALGPRVLPKLPQKVALRKTETSHHLSLDRTNIPPETFQKSSQLTELPQKPPLGELPPKPVELAPKPQVGELPPKPGELPPKPQLGDLPPKPQLSDLPPKPQMKDLPPKPQLGDLLAKSQAGDVSAKVQPPSEVTQRSHTGDLSPNVQSRDAIQKQASEDSNDLTPTLPETPVPLPRKINTGKNKVRRVKTIYDCQADNDDELTFIEGEVIIVTGEEDQEWWIGHIEGQPERKGVFPVSFVHILSD

Target ORF information:

RefSeq Version	XM_006520402.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006520402.4

ORF Insert Sequence:

ATGCAGGCAG GTCTGAGTGG GCCACAGGGC TTGCCTTCCA AAGCAGGCTT TAATGGAGGC 
CAGTCATACG CTTTCAAGGG CTCCTGGAGG CCAATCCCAA GGACTAGGAT GCCGGACCAG 
ATCTCCGTGT CCGAGTTCAT CGCCGAGACC ACCGAGGACT ACAACTCGCC CACCACGTCC 
AGCTTCACCA CGCGGCTGCA CAACTGCAGG AACACCGTCA CGCTGCTGGA GGAGGCTCTA 
GACCAAGATA GAACAGCCCT ACAGAAAGTG AAGAAATCTG TAAAAGCAAT ATATAATTCC 
GGTCAAGATC ATGTACAAAA TGAAGAAAAC TATGCACAAG TTCTGGATAA GTTTGGAAGT 
AATTTTTTAA GCAGAGACAA CCCTGACCTT GGCACCGCTT TTGTCAAGTT TTCTACACTC 
ACAAAGGAAC TGTCCACGCT GCTGAAAAAT CTGCTCCAGG GTCTGAGCCA CAATGTGATC 
TTCACCTTGG ATTCCTTGTT AAAAGGAGAC TTGAAGGGGG TCAAAGGAGA TCTCAAGAAG 
CCATTTGACA AAGCCTGGAA AGATTATGAG ACGAAGTTTA CAAAAATTGA GAAGGAGAAA 
AGAGAGCACG CAAAACAGCA CGGGATGATC CGAACAGAGA TAACAGGCGC TGAGATTGCA 
GAAGAAATGG AAAAGGAACG GCGGCTTTTT CAGCTCCAGA TGTGTGAATA TCTCATTAAA 
GTAAATGAAA TCAAGACCAA AAAGGGTGTG GACCTGCTGC AGAACCTTAT AAAGTATTAC 
CATGCACAGT GCAATTTCTT TCAGGATGGC TTGAAAACGG CTGATAAACT GAAACAGTAC 
ATAGAAAAGC TGGCTGCTGA CTTATATAAT ATAAAGCAAA CCCAAGATGA AGAAAAAAAA 
CAGCTAACTG CACTCCGAGA TCTAATAAAA TCCTCTCTGC AACTTGATCC GAAAGAAGTA 
GGTGGTTTAT ATGTTGCCAG CAGGGCTAAC AGTTCTAGAA GAGACTCTCA GAGCCGGCAA 
GGTGGGTACA GCATGCATCA GCTCCAGGGC AACAAGGAGT ACGGCAGTGA AAAGAAGGGC 
TTCCTGCTGA AGAAGAGTGA TGGGATCCGG AAAGTGTGGC AGAGACGGAA GTGTGCGGTC 
AAGAACGGGA TCCTGACCAT CTCCCACGCA ACTTCCAACA GGCAGCCTGC TAAGTTAAAC 
CTCCTCACCT GCCAAGTGAA ACCGAATGCT GAGGACAAGA AGTCTTTTGA CCTGATATCA 
CATAATCGAA CATATCACTT TCAAGCAGAA GATGAGCAGG ATTATATAGC GTGGATATCA 
GTACTGACGA ATAGCAAAGA AGAGGCCCTA ACCATGGCCT TCCGTGGTGA GCAGAGCACA 
GGGGAGAATA GCCTGGAGGA CCTTACCAAA GCCATCATCG AGGATGTACA GCGGCTCCCT 
GGGAATGACA TCTGCTGTGA CTGCGGCTCA TCAGAACCCA CGTGGCTTTC AACCAACTTG 
GGTATTTTGA CCTGTATAGA ATGTTCCGGA ATCCATAGGG AAATGGGGGT TCATATTTCT 
CGCATTCAGT CTTTGGAACT AGACAAATTA GGAACTTCCG AACTCTTGCT GGCCAAGAAT 
GTAGGAAACA ATAGTTTTAA TGATATTATG GAAGCAAATT TACCCAGCCC TTCACCAAAG 
CCCACCCCTT CAAGTGATAT GACCGTGCGT AAAGAGTACA TCACTGCAAA GTATGTCGAC 
CACCGCTTCT CCAGGAAGAC CTGTGCCTCT TCCTCAGCAA AGCTGAACGA ATTGCTTGAG 
GCCATCAAGT CCAGGGACTT ACTTGCATTA ATCCAAGTCT ATGCAGAGGG GGTGGAGTTA 
ATGGAGCCAC TGCTGGAACC TGGGCAGGAG CTCGGGGAGA CAGCCCTTCA TCTTGCCGTC 
AGGACCGCAG ACCAGACATC TCTCCATCTG GTTGATTTCC TTGTACAAAA CTGTGGGAAC 
CTGGATAAGC AAACGTCTGT GGGGAATACA GTTCTGCACT ATTGCAGCAT GTACGGCAAG 
CCGGAGTGTC TGAAGCTGCT GCTTAGGAGC AAGCCCACCG TGGACATCGT TAACCAGAAT 
GGAGAAACTG CCTTGGATAT AGCAAAGAGA CTCAAAGCTA CCCAGTGTGA AGATCTGCTT 
TCCCAGGCTA AATCTGGAAA GTTCAACCCT CATGTCCACG TAGAGTATGA GTGGAATCTT 
CGACAGGACG AGATGGATGA GAGCGATGAC GATCTGGATG ACAAGCCAAG CCCGATCAAA 
AAGGAACGCT CGCCCAGACC ACAGAGCTTC TGCCACTCCT CCAGCATCTC GCCACAGGAC 
AAGCTGGCAC TGCCAGGGTT CAGCACTCCA CGGGACAAGC AGCGGCTCTC CTACGGAGCC 
TTCACCAACC AGATCTTCGC CTCTACGAGC ACAGATTTGC CCACATCACC CACCAGTGAG 
GCTCCCCCTT TGCCACCTCG GAACGCCGGG AAAGGTAATG ATGTGGGCCC ATTATCTTCA 
AGTAAGACGG CCAACAAGTT TGAGGGGCTG TCTCAGCAAG CAAGCACCAG TTCTGCTAAG 
ACTGCCCTTG GCCCGAGAGT GCTTCCTAAA CTACCTCAGA AAGTGGCACT AAGGAAGACG 
GAGACCAGCC ATCATCTCTC CCTCGACAGA ACCAACATCC CACCTGAGAC TTTTCAGAAA 
TCATCACAGT TGACAGAGTT ACCCCAAAAG CCACCACTTG GAGAGCTGCC CCCGAAGCCT 
GTGGAACTGG CCCCCAAGCC CCAAGTTGGA GAGCTGCCAC CTAAGCCTGG AGAGCTACCC 
CCTAAGCCCC AATTAGGTGA CCTGCCCCCC AAGCCACAGC TCTCAGACTT ACCTCCCAAG 
CCACAGATGA AGGACCTGCC CCCTAAGCCG CAGCTGGGGG ATCTGCTGGC AAAGTCCCAG 
GCTGGCGATG TCTCAGCCAA GGTGCAGCCA CCCTCAGAGG TCACACAGAG GTCACACACC 
GGGGATCTGT CTCCAAATGT ACAGTCCAGA GATGCCATCC AGAAGCAAGC ATCTGAAGAC 
TCCAACGACC TCACACCCAC GCTGCCAGAG ACACCCGTAC CACTGCCGAG AAAAATCAAT 
ACGGGGAAAA ATAAAGTGAG GCGGGTGAAG ACCATTTATG ACTGCCAGGC AGATAATGAC 
GACGAACTCA CATTTATTGA GGGGGAAGTG ATCATTGTCA CCGGGGAAGA GGACCAGGAG 
TGGTGGATCG GGCATATCGA AGGACAGCCT GAAAGGAAGG GTGTCTTTCC AGTGTCCTTT 
GTCCACATCC TGTCTGACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asap1 ( XM_006520403.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006520466.1 Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OMu50924
Clone ID Related Accession (Same CDS sequence)	XM_006520403.3 , XM_006520403.4
Accession Version	XM_006520403.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3312bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006520403.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCAG GTCTGAGTGG GCCACAGGGC TTGCCTTCCA AAGCAGGCTT TAATGGAGGC 
CAGTCATACG CTTTCAAGGG CTCCTGGAGG CCAATCCCAA GGACTAGGAT GCCGGACCAG 
ATCTCCGTGT CCGAGTTCAT CGCCGAGACC ACCGAGGACT ACAACTCGCC CACCACGTCC 
AGCTTCACCA CGCGGCTGCA CAACTGCAGG AACACCGTCA CGCTGCTGGA GGAGGCTCTA 
GACCAAGATA GAACAGCCCT ACAGAAAGTG AAGAAATCTG TAAAAGCAAT ATATAATTCC 
GGTCAAGATC ATGTACAAAA TGAAGAAAAC TATGCACAAG TTCTGGATAA GTTTGGAAGT 
AATTTTTTAA GCAGAGACAA CCCTGACCTT GGCACCGCTT TTGTCAAGTT TTCTACACTC 
ACAAAGGAAC TGTCCACGCT GCTGAAAAAT CTGCTCCAGG GTCTGAGCCA CAATGTGATC 
TTCACCTTGG ATTCCTTGTT AAAAGGAGAC TTGAAGGGGG TCAAAGGAGA TCTCAAGAAG 
CCATTTGACA AAGCCTGGAA AGATTATGAG ACGAAGTTTA CAAAAATTGA GAAGGAGAAA 
AGAGAGCACG CAAAACAGCA CGGGATGATC CGAACAGAGA TAACAGGCGC TGAGATTGCA 
GAAGAAATGG AAAAGGAACG GCGGCTTTTT CAGCTCCAGA TGTGTGAATA TCTCATTAAA 
GTAAATGAAA TCAAGACCAA AAAGGGTGTG GACCTGCTGC AGAACCTTAT AAAGTATTAC 
CATGCACAGT GCAATTTCTT TCAGGATGGC TTGAAAACGG CTGATAAACT GAAACAGTAC 
ATAGAAAAGC TGGCTGCTGA CTTATATAAT ATAAAGCAAA CCCAAGATGA AGAAAAAAAA 
CAGCTAACTG CACTCCGAGA TCTAATAAAA TCCTCTCTGC AACTTGATCC GAAAGAAGTA 
GGTGGTTTAT ATGTTGCCAG CAGGGCTAAC AGTGACTCTC AGAGCCGGCA AGGTGGGTAC 
AGCATGCATC AGCTCCAGGG CAACAAGGAG TACGGCAGTG AAAAGAAGGG CTTCCTGCTG 
AAGAAGAGTG ATGGGATCCG GAAAGTGTGG CAGAGACGGA AGTGTGCGGT CAAGAACGGG 
ATCCTGACCA TCTCCCACGC AACTTCCAAC AGGCAGCCTG CTAAGTTAAA CCTCCTCACC 
TGCCAAGTGA AACCGAATGC TGAGGACAAG AAGTCTTTTG ACCTGATATC ACATAATCGA 
ACATATCACT TTCAAGCAGA AGATGAGCAG GATTATATAG CGTGGATATC AGTACTGACG 
AATAGCAAAG AAGAGGCCCT AACCATGGCC TTCCGTGGTG AGCAGAGCAC AGGGGAGAAT 
AGCCTGGAGG ACCTTACCAA AGCCATCATC GAGGATGTAC AGCGGCTCCC TGGGAATGAC 
ATCTGCTGTG ACTGCGGCTC ATCAGAACCC ACGTGGCTTT CAACCAACTT GGGTATTTTG 
ACCTGTATAG AATGTTCCGG AATCCATAGG GAAATGGGGG TTCATATTTC TCGCATTCAG 
TCTTTGGAAC TAGACAAATT AGGAACTTCC GAACTCTTGC TGGCCAAGAA TGTAGGAAAC 
AATAGTTTTA ATGATATTAT GGAAGCAAAT TTACCCAGCC CTTCACCAAA GCCCACCCCT 
TCAAGTGATA TGACCGTGCG TAAAGAGTAC ATCACTGCAA AGTATGTCGA CCACCGCTTC 
TCCAGGAAGA CCTGTGCCTC TTCCTCAGCA AAGCTGAACG AATTGCTTGA GGCCATCAAG 
TCCAGGGACT TACTTGCATT AATCCAAGTC TATGCAGAGG GGGTGGAGTT AATGGAGCCA 
CTGCTGGAAC CTGGGCAGGA GCTCGGGGAG ACAGCCCTTC ATCTTGCCGT CAGGACCGCA 
GACCAGACAT CTCTCCATCT GGTTGATTTC CTTGTACAAA ACTGTGGGAA CCTGGATAAG 
CAAACGTCTG TGGGGAATAC AGTTCTGCAC TATTGCAGCA TGTACGGCAA GCCGGAGTGT 
CTGAAGCTGC TGCTTAGGAG CAAGCCCACC GTGGACATCG TTAACCAGAA TGGAGAAACT 
GCCTTGGATA TAGCAAAGAG ACTCAAAGCT ACCCAGTGTG AAGATCTGCT TTCCCAGGCT 
AAATCTGGAA AGTTCAACCC TCATGTCCAC GTAGAGTATG AGTGGAATCT TCGACAGGAC 
GAGATGGATG AGAGCGATGA CGATCTGGAT GACAAGCCAA GCCCGATCAA AAAGGAACGC 
TCGCCCAGAC CACAGAGCTT CTGCCACTCC TCCAGCATCT CGCCACAGGA CAAGCTGGCA 
CTGCCAGGGT TCAGCACTCC ACGGGACAAG CAGCGGCTCT CCTACGGAGC CTTCACCAAC 
CAGATCTTCG CCTCTACGAG CACAGATTTG CCCACATCAC CCACCAGTGA GGCTCCCCCT 
TTGCCACCTC GGAACGCCGG GAAAGGTAAT GATGTGGGCC CATTATCTTC AAGTAAGACG 
GCCAACAAGT TTGAGGGGCT GTCTCAGCAA GCAAGCACCA GTTCTGCTAA GACTGCCCTT 
GGCCCGAGAG TGCTTCCTAA ACTACCTCAG AAAGTGGCAC TAAGGAAGAC GGAGACCAGC 
CATCATCTCT CCCTCGACAG AACCAACATC CCACCTGAGA CTTTTCAGAA ATCATCACAG 
TTGACAGAGT TACCCCAAAA GCCACCACTT GGAGAGCTGC CCCCGAAGCC TGTGGAACTG 
GCCCCCAAGC CCCAAGTTGG AGAGCTGCCA CCTAAGCCTG GAGAGCTACC CCCTAAGCCC 
CAATTAGGTG ACCTGCCCCC CAAGCCACAG CTCTCAGACT TACCTCCCAA GCCACAGATG 
AAGGACCTGC CCCCTAAGCC GCAGCTGGGG GATCTGCTGG CAAAGTCCCA GGCTGGCGAT 
GTCTCAGCCA AGGTGCAGCC ACCCTCAGAG GTCACACAGA GGTCACACAC CGGGGATCTG 
TCTCCAAATG TACAGTCCAG AGATGCCATC CAGAAGCAAG CATCTGAAGA CTCCAACGAC 
CTCACACCCA CGCTGCCAGA GACACCCGTA CCACTGCCGA GAAAAATCAA TACGGGGAAA 
AATAAAGTGA GGCGGGTGAA GACCATTTAT GACTGCCAGG CAGATAATGA CGACGAACTC 
ACATTTATTG AGGGGGAAGT GATCATTGTC ACCGGGGAAG AGGACCAGGA GTGGTGGATC 
GGGCATATCG AAGGACAGCC TGAAAGGAAG GGTGTCTTTC CAGTGTCCTT TGTCCACATC 
CTGTCTGACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006520466.1
CDS	202..3513
Translation	MQAGLSGPQGLPSKAGFNGGQSYAFKGSWRPIPRTRMPDQISVSEFIAETTEDYNSPTTSSFTTRLHNCRNTVTLLEEALDQDRTALQKVKKSVKAIYNSGQDHVQNEENYAQVLDKFGSNFLSRDNPDLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGVKGDLKKPFDKAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDPKEVGGLYVASRANSDSQSRQGGYSMHQLQGNKEYGSEKKGFLLKKSDGIRKVWQRRKCAVKNGILTISHATSNRQPAKLNLLTCQVKPNAEDKKSFDLISHNRTYHFQAEDEQDYIAWISVLTNSKEEALTMAFRGEQSTGENSLEDLTKAIIEDVQRLPGNDICCDCGSSEPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDKLGTSELLLAKNVGNNSFNDIMEANLPSPSPKPTPSSDMTVRKEYITAKYVDHRFSRKTCASSSAKLNELLEAIKSRDLLALIQVYAEGVELMEPLLEPGQELGETALHLAVRTADQTSLHLVDFLVQNCGNLDKQTSVGNTVLHYCSMYGKPECLKLLLRSKPTVDIVNQNGETALDIAKRLKATQCEDLLSQAKSGKFNPHVHVEYEWNLRQDEMDESDDDLDDKPSPIKKERSPRPQSFCHSSSISPQDKLALPGFSTPRDKQRLSYGAFTNQIFASTSTDLPTSPTSEAPPLPPRNAGKGNDVGPLSSSKTANKFEGLSQQASTSSAKTALGPRVLPKLPQKVALRKTETSHHLSLDRTNIPPETFQKSSQLTELPQKPPLGELPPKPVELAPKPQVGELPPKPGELPPKPQLGDLPPKPQLSDLPPKPQMKDLPPKPQLGDLLAKSQAGDVSAKVQPPSEVTQRSHTGDLSPNVQSRDAIQKQASEDSNDLTPTLPETPVPLPRKINTGKNKVRRVKTIYDCQADNDDELTFIEGEVIIVTGEEDQEWWIGHIEGQPERKGVFPVSFVHILSD

Target ORF information:

RefSeq Version	XM_006520403.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006520403.4

ORF Insert Sequence:

ATGCAGGCAG GTCTGAGTGG GCCACAGGGC TTGCCTTCCA AAGCAGGCTT TAATGGAGGC 
CAGTCATACG CTTTCAAGGG CTCCTGGAGG CCAATCCCAA GGACTAGGAT GCCGGACCAG 
ATCTCCGTGT CCGAGTTCAT CGCCGAGACC ACCGAGGACT ACAACTCGCC CACCACGTCC 
AGCTTCACCA CGCGGCTGCA CAACTGCAGG AACACCGTCA CGCTGCTGGA GGAGGCTCTA 
GACCAAGATA GAACAGCCCT ACAGAAAGTG AAGAAATCTG TAAAAGCAAT ATATAATTCC 
GGTCAAGATC ATGTACAAAA TGAAGAAAAC TATGCACAAG TTCTGGATAA GTTTGGAAGT 
AATTTTTTAA GCAGAGACAA CCCTGACCTT GGCACCGCTT TTGTCAAGTT TTCTACACTC 
ACAAAGGAAC TGTCCACGCT GCTGAAAAAT CTGCTCCAGG GTCTGAGCCA CAATGTGATC 
TTCACCTTGG ATTCCTTGTT AAAAGGAGAC TTGAAGGGGG TCAAAGGAGA TCTCAAGAAG 
CCATTTGACA AAGCCTGGAA AGATTATGAG ACGAAGTTTA CAAAAATTGA GAAGGAGAAA 
AGAGAGCACG CAAAACAGCA CGGGATGATC CGAACAGAGA TAACAGGCGC TGAGATTGCA 
GAAGAAATGG AAAAGGAACG GCGGCTTTTT CAGCTCCAGA TGTGTGAATA TCTCATTAAA 
GTAAATGAAA TCAAGACCAA AAAGGGTGTG GACCTGCTGC AGAACCTTAT AAAGTATTAC 
CATGCACAGT GCAATTTCTT TCAGGATGGC TTGAAAACGG CTGATAAACT GAAACAGTAC 
ATAGAAAAGC TGGCTGCTGA CTTATATAAT ATAAAGCAAA CCCAAGATGA AGAAAAAAAA 
CAGCTAACTG CACTCCGAGA TCTAATAAAA TCCTCTCTGC AACTTGATCC GAAAGAAGTA 
GGTGGTTTAT ATGTTGCCAG CAGGGCTAAC AGTGACTCTC AGAGCCGGCA AGGTGGGTAC 
AGCATGCATC AGCTCCAGGG CAACAAGGAG TACGGCAGTG AAAAGAAGGG CTTCCTGCTG 
AAGAAGAGTG ATGGGATCCG GAAAGTGTGG CAGAGACGGA AGTGTGCGGT CAAGAACGGG 
ATCCTGACCA TCTCCCACGC AACTTCCAAC AGGCAGCCTG CTAAGTTAAA CCTCCTCACC 
TGCCAAGTGA AACCGAATGC TGAGGACAAG AAGTCTTTTG ACCTGATATC ACATAATCGA 
ACATATCACT TTCAAGCAGA AGATGAGCAG GATTATATAG CGTGGATATC AGTACTGACG 
AATAGCAAAG AAGAGGCCCT AACCATGGCC TTCCGTGGTG AGCAGAGCAC AGGGGAGAAT 
AGCCTGGAGG ACCTTACCAA AGCCATCATC GAGGATGTAC AGCGGCTCCC TGGGAATGAC 
ATCTGCTGTG ACTGCGGCTC ATCAGAACCC ACGTGGCTTT CAACCAACTT GGGTATTTTG 
ACCTGTATAG AATGTTCCGG AATCCATAGG GAAATGGGGG TTCATATTTC TCGCATTCAG 
TCTTTGGAAC TAGACAAATT AGGAACTTCC GAACTCTTGC TGGCCAAGAA TGTAGGAAAC 
AATAGTTTTA ATGATATTAT GGAAGCAAAT TTACCCAGCC CTTCACCAAA GCCCACCCCT 
TCAAGTGATA TGACCGTGCG TAAAGAGTAC ATCACTGCAA AGTATGTCGA CCACCGCTTC 
TCCAGGAAGA CCTGTGCCTC TTCCTCAGCA AAGCTGAACG AATTGCTTGA GGCCATCAAG 
TCCAGGGACT TACTTGCATT AATCCAAGTC TATGCAGAGG GGGTGGAGTT AATGGAGCCA 
CTGCTGGAAC CTGGGCAGGA GCTCGGGGAG ACAGCCCTTC ATCTTGCCGT CAGGACCGCA 
GACCAGACAT CTCTCCATCT GGTTGATTTC CTTGTACAAA ACTGTGGGAA CCTGGATAAG 
CAAACGTCTG TGGGGAATAC AGTTCTGCAC TATTGCAGCA TGTACGGCAA GCCGGAGTGT 
CTGAAGCTGC TGCTTAGGAG CAAGCCCACC GTGGACATCG TTAACCAGAA TGGAGAAACT 
GCCTTGGATA TAGCAAAGAG ACTCAAAGCT ACCCAGTGTG AAGATCTGCT TTCCCAGGCT 
AAATCTGGAA AGTTCAACCC TCATGTCCAC GTAGAGTATG AGTGGAATCT TCGACAGGAC 
GAGATGGATG AGAGCGATGA CGATCTGGAT GACAAGCCAA GCCCGATCAA AAAGGAACGC 
TCGCCCAGAC CACAGAGCTT CTGCCACTCC TCCAGCATCT CGCCACAGGA CAAGCTGGCA 
CTGCCAGGGT TCAGCACTCC ACGGGACAAG CAGCGGCTCT CCTACGGAGC CTTCACCAAC 
CAGATCTTCG CCTCTACGAG CACAGATTTG CCCACATCAC CCACCAGTGA GGCTCCCCCT 
TTGCCACCTC GGAACGCCGG GAAAGGTAAT GATGTGGGCC CATTATCTTC AAGTAAGACG 
GCCAACAAGT TTGAGGGGCT GTCTCAGCAA GCAAGCACCA GTTCTGCTAA GACTGCCCTT 
GGCCCGAGAG TGCTTCCTAA ACTACCTCAG AAAGTGGCAC TAAGGAAGAC GGAGACCAGC 
CATCATCTCT CCCTCGACAG AACCAACATC CCACCTGAGA CTTTTCAGAA ATCATCACAG 
TTGACAGAGT TACCCCAAAA GCCACCACTT GGAGAGCTGC CCCCGAAGCC TGTGGAACTG 
GCCCCCAAGC CCCAAGTTGG AGAGCTGCCA CCTAAGCCTG GAGAGCTACC CCCTAAGCCC 
CAATTAGGTG ACCTGCCCCC CAAGCCACAG CTCTCAGACT TACCTCCCAA GCCACAGATG 
AAGGACCTGC CCCCTAAGCC GCAGCTGGGG GATCTGCTGG CAAAGTCCCA GGCTGGCGAT 
GTCTCAGCCA AGGTGCAGCC ACCCTCAGAG GTCACACAGA GGTCACACAC CGGGGATCTG 
TCTCCAAATG TACAGTCCAG AGATGCCATC CAGAAGCAAG CATCTGAAGA CTCCAACGAC 
CTCACACCCA CGCTGCCAGA GACACCCGTA CCACTGCCGA GAAAAATCAA TACGGGGAAA 
AATAAAGTGA GGCGGGTGAA GACCATTTAT GACTGCCAGG CAGATAATGA CGACGAACTC 
ACATTTATTG AGGGGGAAGT GATCATTGTC ACCGGGGAAG AGGACCAGGA GTGGTGGATC 
GGGCATATCG AAGGACAGCC TGAAAGGAAG GGTGTCTTTC CAGTGTCCTT TGTCCACATC 
CTGTCTGACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asap1 ( XM_030248315.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030104175.1 Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OMu86631
Clone ID Related Accession (Same CDS sequence)	XM_030248315.1
Accession Version	XM_030248315.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3399bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATCTT CAGCCTCCCG GCTCTCCAGT TTTTCCTCCA GAGATTCACT ATGGAATCGG 
ATGCCGGACC AGATCTCCGT GTCCGAGTTC ATCGCCGAGA CCACCGAGGA CTACAACTCG 
CCCACCACGT CCAGCTTCAC CACGCGGCTG CACAACTGCA GGAACACCGT CACGCTGCTG 
GAGGAGGCTC TAGACCAAGA TAGAACAGCC CTACAGAAAG TGAAGAAATC TGTAAAAGCA 
ATATATAATT CCGGTCAAGA TCATGTACAA AATGAAGAAA ACTATGCACA AGTTCTGGAT 
AAGTTTGGAA GTAATTTTTT AAGCAGAGAC AACCCTGACC TTGGCACCGC TTTTGTCAAG 
TTTTCTACAC TCACAAAGGA ACTGTCCACG CTGCTGAAAA ATCTGCTCCA GGGTCTGAGC 
CACAATGTGA TCTTCACCTT GGATTCCTTG TTAAAAGGAG ACTTGAAGGG GGTCAAAGGA 
GATCTCAAGA AGCCATTTGA CAAAGCCTGG AAAGATTATG AGACGAAGTT TACAAAAATT 
GAGAAGGAGA AAAGAGAGCA CGCAAAACAG CACGGGATGA TCCGAACAGA GATAACAGGC 
GCTGAGATTG CAGAAGAAAT GGAAAAGGAA CGGCGGCTTT TTCAGCTCCA GATGTGTGAA 
TATCTCATTA AAGTAAATGA AATCAAGACC AAAAAGGGTG TGGACCTGCT GCAGAACCTT 
ATAAAGTATT ACCATGCACA GTGCAATTTC TTTCAGGATG GCTTGAAAAC GGCTGATAAA 
CTGAAACAGT ACATAGAAAA GCTGGCTGCT GACTTATATA ATATAAAGCA AACCCAAGAT 
GAAGAAAAAA AACAGCTAAC TGCACTCCGA GATCTAATAA AATCCTCTCT GCAACTTGAT 
CCGAAAGAAG ACTCTCAGAG CCGGCAAGGT GGGTACAGCA TGCATCAGCT CCAGGGCAAC 
AAGGAGTACG GCAGTGAAAA GAAGGGCTTC CTGCTGAAGA AGAGTGATGG GATCCGGAAA 
GTGTGGCAGA GACGGAAGTG TGCGGTCAAG AACGGGATCC TGACCATCTC CCACGCAACT 
TCCAACAGGC AGCCTGCTAA GTTAAACCTC CTCACCTGCC AAGTGAAACC GAATGCTGAG 
GACAAGAAGT CTTTTGACCT GATATCACAT AATCGAACAT ATCACTTTCA AGCAGAAGAT 
GAGCAGGATT ATATAGCGTG GATATCAGTA CTGACGAATA GCAAAGAAGA GGCCCTAACC 
ATGGCCTTCC GTGGTGAGCA GAGCACAGGG GAGAATAGCC TGGAGGACCT TACCAAAGCC 
ATCATCGAGG ATGTACAGCG GCTCCCTGGG AATGACATCT GCTGTGACTG CGGCTCATCA 
GAACCCACGT GGCTTTCAAC CAACTTGGGT ATTTTGACCT GTATAGAATG TTCCGGAATC 
CATAGGGAAA TGGGGGTTCA TATTTCTCGC ATTCAGTCTT TGGAACTAGA CAAATTAGGA 
ACTTCCGAAC TCTTGCTGGC CAAGAATGTA GGAAACAATA GTTTTAATGA TATTATGGAA 
GCAAATTTAC CCAGCCCTTC ACCAAAGCCC ACCCCTTCAA GTGATATGAC CGTGCGTAAA 
GAGTACATCA CTGCAAAGTA TGTCGACCAC CGCTTCTCCA GGAAGACCTG TGCCTCTTCC 
TCAGCAAAGC TGAACGAATT GCTTGAGGCC ATCAAGTCCA GGGACTTACT TGCATTAATC 
CAAGTCTATG CAGAGGGGGT GGAGTTAATG GAGCCACTGC TGGAACCTGG GCAGGAGCTC 
GGGGAGACAG CCCTTCATCT TGCCGTCAGG ACCGCAGACC AGACATCTCT CCATCTGGTT 
GATTTCCTTG TACAAAACTG TGGGAACCTG GATAAGCAAA CGTCTGTGGG GAATACAGTT 
CTGCACTATT GCAGCATGTA CGGCAAGCCG GAGTGTCTGA AGCTGCTGCT TAGGAGCAAG 
CCCACCGTGG ACATCGTTAA CCAGAATGGA GAAACTGCCT TGGATATAGC AAAGAGACTC 
AAAGCTACCC AGTGTGAAGA TCTGCTTTCC CAGGCTAAAT CTGGAAAGTT CAACCCTCAT 
GTCCACGTAG AGTATGAGTG GAATCTTCGA CAGGACGAGA TGGATGAGAG CGATGACGAT 
CTGGATGACA AGCCAAGCCC GATCAAAAAG GAACGCTCGC CCAGACCACA GAGCTTCTGC 
CACTCCTCCA GCATCTCGCC ACAGGACAAG CTGGCACTGC CAGGGTTCAG CACTCCACGG 
GACAAGCAGC GGCTCTCCTA CGGAGCCTTC ACCAACCAGA TCTTCGCCTC TACGAGCACA 
GATTTGCCCA CATCACCCAC CAGTGAGGCT CCCCCTTTGC CACCTCGGAA CGCCGGGAAA 
GGTCCAACTG GCCCACCTTC AACACTCCCT CTAGGCACCC AGACCTCTAG TGGCAGCTCC 
ACCCTATCCA AGAAGAGGCC TCCTCCCCCA CCACCAGGAC ACAAGAGAAC CCTGTCTGAC 
CCTCCCAGCC CACTACCTCA CGGGCCCCCA AACAAAGGCG CAATTCCTTG GGGTAATGAT 
GTGGGCCCAT TATCTTCAAG TAAGACGGCC AACAAGTTTG AGGGGCTGTC TCAGCAAGCA 
AGCACCAGTT CTGCTAAGAC TGCCCTTGGC CCGAGAGTGC TTCCTAAACT ACCTCAGAAA 
GTGGCACTAA GGAAGACGGA GACCAGCCAT CATCTCTCCC TCGACAGAAC CAACATCCCA 
CCTGAGACTT TTCAGAAATC ATCACAGTTG ACAGAGTTAC CCCAAAAGCC ACCACTTGGA 
GAGCTGCCCC CGAAGCCTGT GGAACTGGCC CCCAAGCCCC AAGTTGGAGA GCTGCCACCT 
AAGCCTGGAG AGCTACCCCC TAAGCCCCAA TTAGGTGACC TGCCCCCCAA GCCACAGCTC 
TCAGACTTAC CTCCCAAGCC ACAGATGAAG GACCTGCCCC CTAAGCCGCA GCTGGGGGAT 
CTGCTGGCAA AGTCCCAGGC TGGCGATGTC TCAGCCAAGG TGCAGCCACC CTCAGAGGTC 
ACACAGAGGT CACACACCGG GGATCTGTCT CCAAATGTAC AGTCCAGAGA TGCCATCCAG 
AAGCAAGCAT CTGAAGACTC CAACGACCTC ACACCCACGC TGCCAGAGAC ACCCGTACCA 
CTGCCGAGAA AAATCAATAC GGGGAAAAAT AAAGTGAGGC GGGTGAAGAC CATTTATGAC 
TGCCAGGCAG ATAATGACGA CGAACTCACA TTTATTGAGG GGGAAGTGAT CATTGTCACC 
GGGGAAGAGG ACCAGGAGTG GTGGATCGGG CATATCGAAG GACAGCCTGA AAGGAAGGGT 
GTCTTTCCAG TGTCCTTTGT CCACATCCTG TCTGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030104175.1
CDS	253..3651
Translation	MRSSASRLSSFSSRDSLWNRMPDQISVSEFIAETTEDYNSPTTSSFTTRLHNCRNTVTLLEEALDQDRTALQKVKKSVKAIYNSGQDHVQNEENYAQVLDKFGSNFLSRDNPDLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGVKGDLKKPFDKAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDPKEDSQSRQGGYSMHQLQGNKEYGSEKKGFLLKKSDGIRKVWQRRKCAVKNGILTISHATSNRQPAKLNLLTCQVKPNAEDKKSFDLISHNRTYHFQAEDEQDYIAWISVLTNSKEEALTMAFRGEQSTGENSLEDLTKAIIEDVQRLPGNDICCDCGSSEPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDKLGTSELLLAKNVGNNSFNDIMEANLPSPSPKPTPSSDMTVRKEYITAKYVDHRFSRKTCASSSAKLNELLEAIKSRDLLALIQVYAEGVELMEPLLEPGQELGETALHLAVRTADQTSLHLVDFLVQNCGNLDKQTSVGNTVLHYCSMYGKPECLKLLLRSKPTVDIVNQNGETALDIAKRLKATQCEDLLSQAKSGKFNPHVHVEYEWNLRQDEMDESDDDLDDKPSPIKKERSPRPQSFCHSSSISPQDKLALPGFSTPRDKQRLSYGAFTNQIFASTSTDLPTSPTSEAPPLPPRNAGKGPTGPPSTLPLGTQTSSGSSTLSKKRPPPPPPGHKRTLSDPPSPLPHGPPNKGAIPWGNDVGPLSSSKTANKFEGLSQQASTSSAKTALGPRVLPKLPQKVALRKTETSHHLSLDRTNIPPETFQKSSQLTELPQKPPLGELPPKPVELAPKPQVGELPPKPGELPPKPQLGDLPPKPQLSDLPPKPQMKDLPPKPQLGDLLAKSQAGDVSAKVQPPSEVTQRSHTGDLSPNVQSRDAIQKQASEDSNDLTPTLPETPVPLPRKINTGKNKVRRVKTIYDCQADNDDELTFIEGEVIIVTGEEDQEWWIGHIEGQPERKGVFPVSFVHILSD

Target ORF information:

RefSeq Version	XM_030248315.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030248315.1

ORF Insert Sequence:

ATGAGATCTT CAGCCTCCCG GCTCTCCAGT TTTTCCTCCA GAGATTCACT ATGGAATCGG 
ATGCCGGACC AGATCTCCGT GTCCGAGTTC ATCGCCGAGA CCACCGAGGA CTACAACTCG 
CCCACCACGT CCAGCTTCAC CACGCGGCTG CACAACTGCA GGAACACCGT CACGCTGCTG 
GAGGAGGCTC TAGACCAAGA TAGAACAGCC CTACAGAAAG TGAAGAAATC TGTAAAAGCA 
ATATATAATT CCGGTCAAGA TCATGTACAA AATGAAGAAA ACTATGCACA AGTTCTGGAT 
AAGTTTGGAA GTAATTTTTT AAGCAGAGAC AACCCTGACC TTGGCACCGC TTTTGTCAAG 
TTTTCTACAC TCACAAAGGA ACTGTCCACG CTGCTGAAAA ATCTGCTCCA GGGTCTGAGC 
CACAATGTGA TCTTCACCTT GGATTCCTTG TTAAAAGGAG ACTTGAAGGG GGTCAAAGGA 
GATCTCAAGA AGCCATTTGA CAAAGCCTGG AAAGATTATG AGACGAAGTT TACAAAAATT 
GAGAAGGAGA AAAGAGAGCA CGCAAAACAG CACGGGATGA TCCGAACAGA GATAACAGGC 
GCTGAGATTG CAGAAGAAAT GGAAAAGGAA CGGCGGCTTT TTCAGCTCCA GATGTGTGAA 
TATCTCATTA AAGTAAATGA AATCAAGACC AAAAAGGGTG TGGACCTGCT GCAGAACCTT 
ATAAAGTATT ACCATGCACA GTGCAATTTC TTTCAGGATG GCTTGAAAAC GGCTGATAAA 
CTGAAACAGT ACATAGAAAA GCTGGCTGCT GACTTATATA ATATAAAGCA AACCCAAGAT 
GAAGAAAAAA AACAGCTAAC TGCACTCCGA GATCTAATAA AATCCTCTCT GCAACTTGAT 
CCGAAAGAAG ACTCTCAGAG CCGGCAAGGT GGGTACAGCA TGCATCAGCT CCAGGGCAAC 
AAGGAGTACG GCAGTGAAAA GAAGGGCTTC CTGCTGAAGA AGAGTGATGG GATCCGGAAA 
GTGTGGCAGA GACGGAAGTG TGCGGTCAAG AACGGGATCC TGACCATCTC CCACGCAACT 
TCCAACAGGC AGCCTGCTAA GTTAAACCTC CTCACCTGCC AAGTGAAACC GAATGCTGAG 
GACAAGAAGT CTTTTGACCT GATATCACAT AATCGAACAT ATCACTTTCA AGCAGAAGAT 
GAGCAGGATT ATATAGCGTG GATATCAGTA CTGACGAATA GCAAAGAAGA GGCCCTAACC 
ATGGCCTTCC GTGGTGAGCA GAGCACAGGG GAGAATAGCC TGGAGGACCT TACCAAAGCC 
ATCATCGAGG ATGTACAGCG GCTCCCTGGG AATGACATCT GCTGTGACTG CGGCTCATCA 
GAACCCACGT GGCTTTCAAC CAACTTGGGT ATTTTGACCT GTATAGAATG TTCCGGAATC 
CATAGGGAAA TGGGGGTTCA TATTTCTCGC ATTCAGTCTT TGGAACTAGA CAAATTAGGA 
ACTTCCGAAC TCTTGCTGGC CAAGAATGTA GGAAACAATA GTTTTAATGA TATTATGGAA 
GCAAATTTAC CCAGCCCTTC ACCAAAGCCC ACCCCTTCAA GTGATATGAC CGTGCGTAAA 
GAGTACATCA CTGCAAAGTA TGTCGACCAC CGCTTCTCCA GGAAGACCTG TGCCTCTTCC 
TCAGCAAAGC TGAACGAATT GCTTGAGGCC ATCAAGTCCA GGGACTTACT TGCATTAATC 
CAAGTCTATG CAGAGGGGGT GGAGTTAATG GAGCCACTGC TGGAACCTGG GCAGGAGCTC 
GGGGAGACAG CCCTTCATCT TGCCGTCAGG ACCGCAGACC AGACATCTCT CCATCTGGTT 
GATTTCCTTG TACAAAACTG TGGGAACCTG GATAAGCAAA CGTCTGTGGG GAATACAGTT 
CTGCACTATT GCAGCATGTA CGGCAAGCCG GAGTGTCTGA AGCTGCTGCT TAGGAGCAAG 
CCCACCGTGG ACATCGTTAA CCAGAATGGA GAAACTGCCT TGGATATAGC AAAGAGACTC 
AAAGCTACCC AGTGTGAAGA TCTGCTTTCC CAGGCTAAAT CTGGAAAGTT CAACCCTCAT 
GTCCACGTAG AGTATGAGTG GAATCTTCGA CAGGACGAGA TGGATGAGAG CGATGACGAT 
CTGGATGACA AGCCAAGCCC GATCAAAAAG GAACGCTCGC CCAGACCACA GAGCTTCTGC 
CACTCCTCCA GCATCTCGCC ACAGGACAAG CTGGCACTGC CAGGGTTCAG CACTCCACGG 
GACAAGCAGC GGCTCTCCTA CGGAGCCTTC ACCAACCAGA TCTTCGCCTC TACGAGCACA 
GATTTGCCCA CATCACCCAC CAGTGAGGCT CCCCCTTTGC CACCTCGGAA CGCCGGGAAA 
GGTCCAACTG GCCCACCTTC AACACTCCCT CTAGGCACCC AGACCTCTAG TGGCAGCTCC 
ACCCTATCCA AGAAGAGGCC TCCTCCCCCA CCACCAGGAC ACAAGAGAAC CCTGTCTGAC 
CCTCCCAGCC CACTACCTCA CGGGCCCCCA AACAAAGGCG CAATTCCTTG GGGTAATGAT 
GTGGGCCCAT TATCTTCAAG TAAGACGGCC AACAAGTTTG AGGGGCTGTC TCAGCAAGCA 
AGCACCAGTT CTGCTAAGAC TGCCCTTGGC CCGAGAGTGC TTCCTAAACT ACCTCAGAAA 
GTGGCACTAA GGAAGACGGA GACCAGCCAT CATCTCTCCC TCGACAGAAC CAACATCCCA 
CCTGAGACTT TTCAGAAATC ATCACAGTTG ACAGAGTTAC CCCAAAAGCC ACCACTTGGA 
GAGCTGCCCC CGAAGCCTGT GGAACTGGCC CCCAAGCCCC AAGTTGGAGA GCTGCCACCT 
AAGCCTGGAG AGCTACCCCC TAAGCCCCAA TTAGGTGACC TGCCCCCCAA GCCACAGCTC 
TCAGACTTAC CTCCCAAGCC ACAGATGAAG GACCTGCCCC CTAAGCCGCA GCTGGGGGAT 
CTGCTGGCAA AGTCCCAGGC TGGCGATGTC TCAGCCAAGG TGCAGCCACC CTCAGAGGTC 
ACACAGAGGT CACACACCGG GGATCTGTCT CCAAATGTAC AGTCCAGAGA TGCCATCCAG 
AAGCAAGCAT CTGAAGACTC CAACGACCTC ACACCCACGC TGCCAGAGAC ACCCGTACCA 
CTGCCGAGAA AAATCAATAC GGGGAAAAAT AAAGTGAGGC GGGTGAAGAC CATTTATGAC 
TGCCAGGCAG ATAATGACGA CGAACTCACA TTTATTGAGG GGGAAGTGAT CATTGTCACC 
GGGGAAGAGG ACCAGGAGTG GTGGATCGGG CATATCGAAG GACAGCCTGA AAGGAAGGGT 
GTCTTTCCAG TGTCCTTTGT CCACATCCTG TCTGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asap1 ( XM_030248316.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030104176.1 Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X15, mRNA.

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CloneID	OMu86632
Clone ID Related Accession (Same CDS sequence)	XM_030248316.1
Accession Version	XM_030248316.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3285bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCAG GTCTGAGTGG GCCACAGGGC TTGCCTTCCA AAGCAGGCTT TAATGGAGGC 
CAGTCATACG CTTTCAAGGG CTCCTGGAGG CCAATCCCAA GGACTAGGAT GCCGGACCAG 
ATCTCCGTGT CCGAGTTCAT CGCCGAGACC ACCGAGGACT ACAACTCGCC CACCACGTCC 
AGCTTCACCA CGCGGCTGCA CAACTGCAGG AACACCGTCA CGCTGCTGGA GGAGGCTCTA 
GACCAAGATA GAACAGCCCT ACAGAAAGTG AAGAAATCTG TAAAAGCAAT ATATAATTCC 
GGTCAAGATC ATGTACAAAA TGAAGAAAAC TATGCACAAG TTCTGGATAA GTTTGGAAGT 
AATTTTTTAA GCAGAGACAA CCCTGACCTT GGCACCGCTT TTGTCAAGTT TTCTACACTC 
ACAAAGGAAC TGTCCACGCT GCTGAAAAAT CTGCTCCAGG GTCTGAGCCA CAATGTGATC 
TTCACCTTGG ATTCCTTGTT AAAAGGAGAC TTGAAGGGGG TCAAAGGAGA TCTCAAGAAG 
CCATTTGACA AAGCCTGGAA AGATTATGAG ACGAAGTTTA CAAAAATTGA GAAGGAGAAA 
AGAGAGCACG CAAAACAGCA CGGGATGATC CGAACAGAGA TAACAGGCGC TGAGATTGCA 
GAAGAAATGG AAAAGGAACG GCGGCTTTTT CAGCTCCAGA TGTGTGAATA TCTCATTAAA 
GTAAATGAAA TCAAGACCAA AAAGGGTGTG GACCTGCTGC AGAACCTTAT AAAGTATTAC 
CATGCACAGT GCAATTTCTT TCAGGATGGC TTGAAAACGG CTGATAAACT GAAACAGTAC 
ATAGAAAAGC TGGCTGCTGA CTTATATAAT ATAAAGCAAA CCCAAGATGA AGAAAAAAAA 
CAGCTAACTG CACTCCGAGA TCTAATAAAA TCCTCTCTGC AACTTGATCC GAAAGAATCT 
AGAAGAGACT CTCAGAGCCG GCAAGGTGGG TACAGCATGC ATCAGCTCCA GGGCAACAAG 
GAGTACGGCA GTGAAAAGAA GGGCTTCCTG CTGAAGAAGA GTGATGGGAT CCGGAAAGTG 
TGGCAGAGAC GGAAGTGTGC GGTCAAGAAC GGGATCCTGA CCATCTCCCA CGCAACTTCC 
AACAGGCAGC CTGCTAAGTT AAACCTCCTC ACCTGCCAAG TGAAACCGAA TGCTGAGGAC 
AAGAAGTCTT TTGACCTGAT ATCACATAAT CGAACATATC ACTTTCAAGC AGAAGATGAG 
CAGGATTATA TAGCGTGGAT ATCAGTACTG ACGAATAGCA AAGAAGAGGC CCTAACCATG 
GCCTTCCGTG GTGAGCAGAG CACAGGGGAG AATAGCCTGG AGGACCTTAC CAAAGCCATC 
ATCGAGGATG TACAGCGGCT CCCTGGGAAT GACATCTGCT GTGACTGCGG CTCATCAGAA 
CCCACGTGGC TTTCAACCAA CTTGGGTATT TTGACCTGTA TAGAATGTTC CGGAATCCAT 
AGGGAAATGG GGGTTCATAT TTCTCGCATT CAGTCTTTGG AACTAGACAA ATTAGGAACT 
TCCGAACTCT TGCTGGCCAA GAATGTAGGA AACAATAGTT TTAATGATAT TATGGAAGCA 
AATTTACCCA GCCCTTCACC AAAGCCCACC CCTTCAAGTG ATATGACCGT GCGTAAAGAG 
TACATCACTG CAAAGTATGT CGACCACCGC TTCTCCAGGA AGACCTGTGC CTCTTCCTCA 
GCAAAGCTGA ACGAATTGCT TGAGGCCATC AAGTCCAGGG ACTTACTTGC ATTAATCCAA 
GTCTATGCAG AGGGGGTGGA GTTAATGGAG CCACTGCTGG AACCTGGGCA GGAGCTCGGG 
GAGACAGCCC TTCATCTTGC CGTCAGGACC GCAGACCAGA CATCTCTCCA TCTGGTTGAT 
TTCCTTGTAC AAAACTGTGG GAACCTGGAT AAGCAAACGT CTGTGGGGAA TACAGTTCTG 
CACTATTGCA GCATGTACGG CAAGCCGGAG TGTCTGAAGC TGCTGCTTAG GAGCAAGCCC 
ACCGTGGACA TCGTTAACCA GAATGGAGAA ACTGCCTTGG ATATAGCAAA GAGACTCAAA 
GCTACCCAGT GTGAAGATCT GCTTTCCCAG GCTAAATCTG GAAAGTTCAA CCCTCATGTC 
CACGTAGAGT ATGAGTGGAA TCTTCGACAG GACGAGATGG ATGAGAGCGA TGACGATCTG 
GATGACAAGC CAAGCCCGAT CAAAAAGGAA CGCTCGCCCA GACCACAGAG CTTCTGCCAC 
TCCTCCAGCA TCTCGCCACA GGACAAGCTG GCACTGCCAG GGTTCAGCAC TCCACGGGAC 
AAGCAGCGGC TCTCCTACGG AGCCTTCACC AACCAGATCT TCGCCTCTAC GAGCACAGAT 
TTGCCCACAT CACCCACCAG TGAGGCTCCC CCTTTGCCAC CTCGGAACGC CGGGAAAGGT 
AATGATGTGG GCCCATTATC TTCAAGTAAG ACGGCCAACA AGTTTGAGGG GCTGTCTCAG 
CAAGCAAGCA CCAGTTCTGC TAAGACTGCC CTTGGCCCGA GAGTGCTTCC TAAACTACCT 
CAGAAAGTGG CACTAAGGAA GACGGAGACC AGCCATCATC TCTCCCTCGA CAGAACCAAC 
ATCCCACCTG AGACTTTTCA GAAATCATCA CAGTTGACAG AGTTACCCCA AAAGCCACCA 
CTTGGAGAGC TGCCCCCGAA GCCTGTGGAA CTGGCCCCCA AGCCCCAAGT TGGAGAGCTG 
CCACCTAAGC CTGGAGAGCT ACCCCCTAAG CCCCAATTAG GTGACCTGCC CCCCAAGCCA 
CAGCTCTCAG ACTTACCTCC CAAGCCACAG ATGAAGGACC TGCCCCCTAA GCCGCAGCTG 
GGGGATCTGC TGGCAAAGTC CCAGGCTGGC GATGTCTCAG CCAAGGTGCA GCCACCCTCA 
GAGGTCACAC AGAGGTCACA CACCGGGGAT CTGTCTCCAA ATGTACAGTC CAGAGATGCC 
ATCCAGAAGC AAGCATCTGA AGACTCCAAC GACCTCACAC CCACGCTGCC AGAGACACCC 
GTACCACTGC CGAGAAAAAT CAATACGGGG AAAAATAAAG TGAGGCGGGT GAAGACCATT 
TATGACTGCC AGGCAGATAA TGACGACGAA CTCACATTTA TTGAGGGGGA AGTGATCATT 
GTCACCGGGG AAGAGGACCA GGAGTGGTGG ATCGGGCATA TCGAAGGACA GCCTGAAAGG 
AAGGGTGTCT TTCCAGTGTC CTTTGTCCAC ATCCTGTCTG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030104176.1
CDS	202..3486
Translation	MQAGLSGPQGLPSKAGFNGGQSYAFKGSWRPIPRTRMPDQISVSEFIAETTEDYNSPTTSSFTTRLHNCRNTVTLLEEALDQDRTALQKVKKSVKAIYNSGQDHVQNEENYAQVLDKFGSNFLSRDNPDLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGVKGDLKKPFDKAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDPKESRRDSQSRQGGYSMHQLQGNKEYGSEKKGFLLKKSDGIRKVWQRRKCAVKNGILTISHATSNRQPAKLNLLTCQVKPNAEDKKSFDLISHNRTYHFQAEDEQDYIAWISVLTNSKEEALTMAFRGEQSTGENSLEDLTKAIIEDVQRLPGNDICCDCGSSEPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDKLGTSELLLAKNVGNNSFNDIMEANLPSPSPKPTPSSDMTVRKEYITAKYVDHRFSRKTCASSSAKLNELLEAIKSRDLLALIQVYAEGVELMEPLLEPGQELGETALHLAVRTADQTSLHLVDFLVQNCGNLDKQTSVGNTVLHYCSMYGKPECLKLLLRSKPTVDIVNQNGETALDIAKRLKATQCEDLLSQAKSGKFNPHVHVEYEWNLRQDEMDESDDDLDDKPSPIKKERSPRPQSFCHSSSISPQDKLALPGFSTPRDKQRLSYGAFTNQIFASTSTDLPTSPTSEAPPLPPRNAGKGNDVGPLSSSKTANKFEGLSQQASTSSAKTALGPRVLPKLPQKVALRKTETSHHLSLDRTNIPPETFQKSSQLTELPQKPPLGELPPKPVELAPKPQVGELPPKPGELPPKPQLGDLPPKPQLSDLPPKPQMKDLPPKPQLGDLLAKSQAGDVSAKVQPPSEVTQRSHTGDLSPNVQSRDAIQKQASEDSNDLTPTLPETPVPLPRKINTGKNKVRRVKTIYDCQADNDDELTFIEGEVIIVTGEEDQEWWIGHIEGQPERKGVFPVSFVHILSD

Target ORF information:

RefSeq Version	XM_030248316.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030248316.1

ORF Insert Sequence:

ATGCAGGCAG GTCTGAGTGG GCCACAGGGC TTGCCTTCCA AAGCAGGCTT TAATGGAGGC 
CAGTCATACG CTTTCAAGGG CTCCTGGAGG CCAATCCCAA GGACTAGGAT GCCGGACCAG 
ATCTCCGTGT CCGAGTTCAT CGCCGAGACC ACCGAGGACT ACAACTCGCC CACCACGTCC 
AGCTTCACCA CGCGGCTGCA CAACTGCAGG AACACCGTCA CGCTGCTGGA GGAGGCTCTA 
GACCAAGATA GAACAGCCCT ACAGAAAGTG AAGAAATCTG TAAAAGCAAT ATATAATTCC 
GGTCAAGATC ATGTACAAAA TGAAGAAAAC TATGCACAAG TTCTGGATAA GTTTGGAAGT 
AATTTTTTAA GCAGAGACAA CCCTGACCTT GGCACCGCTT TTGTCAAGTT TTCTACACTC 
ACAAAGGAAC TGTCCACGCT GCTGAAAAAT CTGCTCCAGG GTCTGAGCCA CAATGTGATC 
TTCACCTTGG ATTCCTTGTT AAAAGGAGAC TTGAAGGGGG TCAAAGGAGA TCTCAAGAAG 
CCATTTGACA AAGCCTGGAA AGATTATGAG ACGAAGTTTA CAAAAATTGA GAAGGAGAAA 
AGAGAGCACG CAAAACAGCA CGGGATGATC CGAACAGAGA TAACAGGCGC TGAGATTGCA 
GAAGAAATGG AAAAGGAACG GCGGCTTTTT CAGCTCCAGA TGTGTGAATA TCTCATTAAA 
GTAAATGAAA TCAAGACCAA AAAGGGTGTG GACCTGCTGC AGAACCTTAT AAAGTATTAC 
CATGCACAGT GCAATTTCTT TCAGGATGGC TTGAAAACGG CTGATAAACT GAAACAGTAC 
ATAGAAAAGC TGGCTGCTGA CTTATATAAT ATAAAGCAAA CCCAAGATGA AGAAAAAAAA 
CAGCTAACTG CACTCCGAGA TCTAATAAAA TCCTCTCTGC AACTTGATCC GAAAGAATCT 
AGAAGAGACT CTCAGAGCCG GCAAGGTGGG TACAGCATGC ATCAGCTCCA GGGCAACAAG 
GAGTACGGCA GTGAAAAGAA GGGCTTCCTG CTGAAGAAGA GTGATGGGAT CCGGAAAGTG 
TGGCAGAGAC GGAAGTGTGC GGTCAAGAAC GGGATCCTGA CCATCTCCCA CGCAACTTCC 
AACAGGCAGC CTGCTAAGTT AAACCTCCTC ACCTGCCAAG TGAAACCGAA TGCTGAGGAC 
AAGAAGTCTT TTGACCTGAT ATCACATAAT CGAACATATC ACTTTCAAGC AGAAGATGAG 
CAGGATTATA TAGCGTGGAT ATCAGTACTG ACGAATAGCA AAGAAGAGGC CCTAACCATG 
GCCTTCCGTG GTGAGCAGAG CACAGGGGAG AATAGCCTGG AGGACCTTAC CAAAGCCATC 
ATCGAGGATG TACAGCGGCT CCCTGGGAAT GACATCTGCT GTGACTGCGG CTCATCAGAA 
CCCACGTGGC TTTCAACCAA CTTGGGTATT TTGACCTGTA TAGAATGTTC CGGAATCCAT 
AGGGAAATGG GGGTTCATAT TTCTCGCATT CAGTCTTTGG AACTAGACAA ATTAGGAACT 
TCCGAACTCT TGCTGGCCAA GAATGTAGGA AACAATAGTT TTAATGATAT TATGGAAGCA 
AATTTACCCA GCCCTTCACC AAAGCCCACC CCTTCAAGTG ATATGACCGT GCGTAAAGAG 
TACATCACTG CAAAGTATGT CGACCACCGC TTCTCCAGGA AGACCTGTGC CTCTTCCTCA 
GCAAAGCTGA ACGAATTGCT TGAGGCCATC AAGTCCAGGG ACTTACTTGC ATTAATCCAA 
GTCTATGCAG AGGGGGTGGA GTTAATGGAG CCACTGCTGG AACCTGGGCA GGAGCTCGGG 
GAGACAGCCC TTCATCTTGC CGTCAGGACC GCAGACCAGA CATCTCTCCA TCTGGTTGAT 
TTCCTTGTAC AAAACTGTGG GAACCTGGAT AAGCAAACGT CTGTGGGGAA TACAGTTCTG 
CACTATTGCA GCATGTACGG CAAGCCGGAG TGTCTGAAGC TGCTGCTTAG GAGCAAGCCC 
ACCGTGGACA TCGTTAACCA GAATGGAGAA ACTGCCTTGG ATATAGCAAA GAGACTCAAA 
GCTACCCAGT GTGAAGATCT GCTTTCCCAG GCTAAATCTG GAAAGTTCAA CCCTCATGTC 
CACGTAGAGT ATGAGTGGAA TCTTCGACAG GACGAGATGG ATGAGAGCGA TGACGATCTG 
GATGACAAGC CAAGCCCGAT CAAAAAGGAA CGCTCGCCCA GACCACAGAG CTTCTGCCAC 
TCCTCCAGCA TCTCGCCACA GGACAAGCTG GCACTGCCAG GGTTCAGCAC TCCACGGGAC 
AAGCAGCGGC TCTCCTACGG AGCCTTCACC AACCAGATCT TCGCCTCTAC GAGCACAGAT 
TTGCCCACAT CACCCACCAG TGAGGCTCCC CCTTTGCCAC CTCGGAACGC CGGGAAAGGT 
AATGATGTGG GCCCATTATC TTCAAGTAAG ACGGCCAACA AGTTTGAGGG GCTGTCTCAG 
CAAGCAAGCA CCAGTTCTGC TAAGACTGCC CTTGGCCCGA GAGTGCTTCC TAAACTACCT 
CAGAAAGTGG CACTAAGGAA GACGGAGACC AGCCATCATC TCTCCCTCGA CAGAACCAAC 
ATCCCACCTG AGACTTTTCA GAAATCATCA CAGTTGACAG AGTTACCCCA AAAGCCACCA 
CTTGGAGAGC TGCCCCCGAA GCCTGTGGAA CTGGCCCCCA AGCCCCAAGT TGGAGAGCTG 
CCACCTAAGC CTGGAGAGCT ACCCCCTAAG CCCCAATTAG GTGACCTGCC CCCCAAGCCA 
CAGCTCTCAG ACTTACCTCC CAAGCCACAG ATGAAGGACC TGCCCCCTAA GCCGCAGCTG 
GGGGATCTGC TGGCAAAGTC CCAGGCTGGC GATGTCTCAG CCAAGGTGCA GCCACCCTCA 
GAGGTCACAC AGAGGTCACA CACCGGGGAT CTGTCTCCAA ATGTACAGTC CAGAGATGCC 
ATCCAGAAGC AAGCATCTGA AGACTCCAAC GACCTCACAC CCACGCTGCC AGAGACACCC 
GTACCACTGC CGAGAAAAAT CAATACGGGG AAAAATAAAG TGAGGCGGGT GAAGACCATT 
TATGACTGCC AGGCAGATAA TGACGACGAA CTCACATTTA TTGAGGGGGA AGTGATCATT 
GTCACCGGGG AAGAGGACCA GGAGTGGTGG ATCGGGCATA TCGAAGGACA GCCTGAAAGG 
AAGGGTGTCT TTCCAGTGTC CTTTGTCCAC ATCCTGTCTG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asap1 ( XM_030248317.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030104177.1 Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X16, mRNA.

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CloneID	OMu86633
Clone ID Related Accession (Same CDS sequence)	XM_030248317.1
Accession Version	XM_030248317.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3276bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCAG GTCTGAGTGG GCCACAGGGC TTGCCTTCCA AAGCAGGCTT TAATGGAGGC 
CAGTCATACG CTTTCAAGGG CTCCTGGAGG CCAATCCCAA GGACTAGGAT GCCGGACCAG 
ATCTCCGTGT CCGAGTTCAT CGCCGAGACC ACCGAGGACT ACAACTCGCC CACCACGTCC 
AGCTTCACCA CGCGGCTGCA CAACTGCAGG AACACCGTCA CGCTGCTGGA GGAGGCTCTA 
GACCAAGATA GAACAGCCCT ACAGAAAGTG AAGAAATCTG TAAAAGCAAT ATATAATTCC 
GGTCAAGATC ATGTACAAAA TGAAGAAAAC TATGCACAAG TTCTGGATAA GTTTGGAAGT 
AATTTTTTAA GCAGAGACAA CCCTGACCTT GGCACCGCTT TTGTCAAGTT TTCTACACTC 
ACAAAGGAAC TGTCCACGCT GCTGAAAAAT CTGCTCCAGG GTCTGAGCCA CAATGTGATC 
TTCACCTTGG ATTCCTTGTT AAAAGGAGAC TTGAAGGGGG TCAAAGGAGA TCTCAAGAAG 
CCATTTGACA AAGCCTGGAA AGATTATGAG ACGAAGTTTA CAAAAATTGA GAAGGAGAAA 
AGAGAGCACG CAAAACAGCA CGGGATGATC CGAACAGAGA TAACAGGCGC TGAGATTGCA 
GAAGAAATGG AAAAGGAACG GCGGCTTTTT CAGCTCCAGA TGTGTGAATA TCTCATTAAA 
GTAAATGAAA TCAAGACCAA AAAGGGTGTG GACCTGCTGC AGAACCTTAT AAAGTATTAC 
CATGCACAGT GCAATTTCTT TCAGGATGGC TTGAAAACGG CTGATAAACT GAAACAGTAC 
ATAGAAAAGC TGGCTGCTGA CTTATATAAT ATAAAGCAAA CCCAAGATGA AGAAAAAAAA 
CAGCTAACTG CACTCCGAGA TCTAATAAAA TCCTCTCTGC AACTTGATCC GAAAGAAGAC 
TCTCAGAGCC GGCAAGGTGG GTACAGCATG CATCAGCTCC AGGGCAACAA GGAGTACGGC 
AGTGAAAAGA AGGGCTTCCT GCTGAAGAAG AGTGATGGGA TCCGGAAAGT GTGGCAGAGA 
CGGAAGTGTG CGGTCAAGAA CGGGATCCTG ACCATCTCCC ACGCAACTTC CAACAGGCAG 
CCTGCTAAGT TAAACCTCCT CACCTGCCAA GTGAAACCGA ATGCTGAGGA CAAGAAGTCT 
TTTGACCTGA TATCACATAA TCGAACATAT CACTTTCAAG CAGAAGATGA GCAGGATTAT 
ATAGCGTGGA TATCAGTACT GACGAATAGC AAAGAAGAGG CCCTAACCAT GGCCTTCCGT 
GGTGAGCAGA GCACAGGGGA GAATAGCCTG GAGGACCTTA CCAAAGCCAT CATCGAGGAT 
GTACAGCGGC TCCCTGGGAA TGACATCTGC TGTGACTGCG GCTCATCAGA ACCCACGTGG 
CTTTCAACCA ACTTGGGTAT TTTGACCTGT ATAGAATGTT CCGGAATCCA TAGGGAAATG 
GGGGTTCATA TTTCTCGCAT TCAGTCTTTG GAACTAGACA AATTAGGAAC TTCCGAACTC 
TTGCTGGCCA AGAATGTAGG AAACAATAGT TTTAATGATA TTATGGAAGC AAATTTACCC 
AGCCCTTCAC CAAAGCCCAC CCCTTCAAGT GATATGACCG TGCGTAAAGA GTACATCACT 
GCAAAGTATG TCGACCACCG CTTCTCCAGG AAGACCTGTG CCTCTTCCTC AGCAAAGCTG 
AACGAATTGC TTGAGGCCAT CAAGTCCAGG GACTTACTTG CATTAATCCA AGTCTATGCA 
GAGGGGGTGG AGTTAATGGA GCCACTGCTG GAACCTGGGC AGGAGCTCGG GGAGACAGCC 
CTTCATCTTG CCGTCAGGAC CGCAGACCAG ACATCTCTCC ATCTGGTTGA TTTCCTTGTA 
CAAAACTGTG GGAACCTGGA TAAGCAAACG TCTGTGGGGA ATACAGTTCT GCACTATTGC 
AGCATGTACG GCAAGCCGGA GTGTCTGAAG CTGCTGCTTA GGAGCAAGCC CACCGTGGAC 
ATCGTTAACC AGAATGGAGA AACTGCCTTG GATATAGCAA AGAGACTCAA AGCTACCCAG 
TGTGAAGATC TGCTTTCCCA GGCTAAATCT GGAAAGTTCA ACCCTCATGT CCACGTAGAG 
TATGAGTGGA ATCTTCGACA GGACGAGATG GATGAGAGCG ATGACGATCT GGATGACAAG 
CCAAGCCCGA TCAAAAAGGA ACGCTCGCCC AGACCACAGA GCTTCTGCCA CTCCTCCAGC 
ATCTCGCCAC AGGACAAGCT GGCACTGCCA GGGTTCAGCA CTCCACGGGA CAAGCAGCGG 
CTCTCCTACG GAGCCTTCAC CAACCAGATC TTCGCCTCTA CGAGCACAGA TTTGCCCACA 
TCACCCACCA GTGAGGCTCC CCCTTTGCCA CCTCGGAACG CCGGGAAAGG TAATGATGTG 
GGCCCATTAT CTTCAAGTAA GACGGCCAAC AAGTTTGAGG GGCTGTCTCA GCAAGCAAGC 
ACCAGTTCTG CTAAGACTGC CCTTGGCCCG AGAGTGCTTC CTAAACTACC TCAGAAAGTG 
GCACTAAGGA AGACGGAGAC CAGCCATCAT CTCTCCCTCG ACAGAACCAA CATCCCACCT 
GAGACTTTTC AGAAATCATC ACAGTTGACA GAGTTACCCC AAAAGCCACC ACTTGGAGAG 
CTGCCCCCGA AGCCTGTGGA ACTGGCCCCC AAGCCCCAAG TTGGAGAGCT GCCACCTAAG 
CCTGGAGAGC TACCCCCTAA GCCCCAATTA GGTGACCTGC CCCCCAAGCC ACAGCTCTCA 
GACTTACCTC CCAAGCCACA GATGAAGGAC CTGCCCCCTA AGCCGCAGCT GGGGGATCTG 
CTGGCAAAGT CCCAGGCTGG CGATGTCTCA GCCAAGGTGC AGCCACCCTC AGAGGTCACA 
CAGAGGTCAC ACACCGGGGA TCTGTCTCCA AATGTACAGT CCAGAGATGC CATCCAGAAG 
CAAGCATCTG AAGACTCCAA CGACCTCACA CCCACGCTGC CAGAGACACC CGTACCACTG 
CCGAGAAAAA TCAATACGGG GAAAAATAAA GTGAGGCGGG TGAAGACCAT TTATGACTGC 
CAGGCAGATA ATGACGACGA ACTCACATTT ATTGAGGGGG AAGTGATCAT TGTCACCGGG 
GAAGAGGACC AGGAGTGGTG GATCGGGCAT ATCGAAGGAC AGCCTGAAAG GAAGGGTGTC 
TTTCCAGTGT CCTTTGTCCA CATCCTGTCT GACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030104177.1
CDS	202..3477
Translation	MQAGLSGPQGLPSKAGFNGGQSYAFKGSWRPIPRTRMPDQISVSEFIAETTEDYNSPTTSSFTTRLHNCRNTVTLLEEALDQDRTALQKVKKSVKAIYNSGQDHVQNEENYAQVLDKFGSNFLSRDNPDLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGVKGDLKKPFDKAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDPKEDSQSRQGGYSMHQLQGNKEYGSEKKGFLLKKSDGIRKVWQRRKCAVKNGILTISHATSNRQPAKLNLLTCQVKPNAEDKKSFDLISHNRTYHFQAEDEQDYIAWISVLTNSKEEALTMAFRGEQSTGENSLEDLTKAIIEDVQRLPGNDICCDCGSSEPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDKLGTSELLLAKNVGNNSFNDIMEANLPSPSPKPTPSSDMTVRKEYITAKYVDHRFSRKTCASSSAKLNELLEAIKSRDLLALIQVYAEGVELMEPLLEPGQELGETALHLAVRTADQTSLHLVDFLVQNCGNLDKQTSVGNTVLHYCSMYGKPECLKLLLRSKPTVDIVNQNGETALDIAKRLKATQCEDLLSQAKSGKFNPHVHVEYEWNLRQDEMDESDDDLDDKPSPIKKERSPRPQSFCHSSSISPQDKLALPGFSTPRDKQRLSYGAFTNQIFASTSTDLPTSPTSEAPPLPPRNAGKGNDVGPLSSSKTANKFEGLSQQASTSSAKTALGPRVLPKLPQKVALRKTETSHHLSLDRTNIPPETFQKSSQLTELPQKPPLGELPPKPVELAPKPQVGELPPKPGELPPKPQLGDLPPKPQLSDLPPKPQMKDLPPKPQLGDLLAKSQAGDVSAKVQPPSEVTQRSHTGDLSPNVQSRDAIQKQASEDSNDLTPTLPETPVPLPRKINTGKNKVRRVKTIYDCQADNDDELTFIEGEVIIVTGEEDQEWWIGHIEGQPERKGVFPVSFVHILSD

Target ORF information:

RefSeq Version	XM_030248317.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030248317.1

ORF Insert Sequence:

ATGCAGGCAG GTCTGAGTGG GCCACAGGGC TTGCCTTCCA AAGCAGGCTT TAATGGAGGC 
CAGTCATACG CTTTCAAGGG CTCCTGGAGG CCAATCCCAA GGACTAGGAT GCCGGACCAG 
ATCTCCGTGT CCGAGTTCAT CGCCGAGACC ACCGAGGACT ACAACTCGCC CACCACGTCC 
AGCTTCACCA CGCGGCTGCA CAACTGCAGG AACACCGTCA CGCTGCTGGA GGAGGCTCTA 
GACCAAGATA GAACAGCCCT ACAGAAAGTG AAGAAATCTG TAAAAGCAAT ATATAATTCC 
GGTCAAGATC ATGTACAAAA TGAAGAAAAC TATGCACAAG TTCTGGATAA GTTTGGAAGT 
AATTTTTTAA GCAGAGACAA CCCTGACCTT GGCACCGCTT TTGTCAAGTT TTCTACACTC 
ACAAAGGAAC TGTCCACGCT GCTGAAAAAT CTGCTCCAGG GTCTGAGCCA CAATGTGATC 
TTCACCTTGG ATTCCTTGTT AAAAGGAGAC TTGAAGGGGG TCAAAGGAGA TCTCAAGAAG 
CCATTTGACA AAGCCTGGAA AGATTATGAG ACGAAGTTTA CAAAAATTGA GAAGGAGAAA 
AGAGAGCACG CAAAACAGCA CGGGATGATC CGAACAGAGA TAACAGGCGC TGAGATTGCA 
GAAGAAATGG AAAAGGAACG GCGGCTTTTT CAGCTCCAGA TGTGTGAATA TCTCATTAAA 
GTAAATGAAA TCAAGACCAA AAAGGGTGTG GACCTGCTGC AGAACCTTAT AAAGTATTAC 
CATGCACAGT GCAATTTCTT TCAGGATGGC TTGAAAACGG CTGATAAACT GAAACAGTAC 
ATAGAAAAGC TGGCTGCTGA CTTATATAAT ATAAAGCAAA CCCAAGATGA AGAAAAAAAA 
CAGCTAACTG CACTCCGAGA TCTAATAAAA TCCTCTCTGC AACTTGATCC GAAAGAAGAC 
TCTCAGAGCC GGCAAGGTGG GTACAGCATG CATCAGCTCC AGGGCAACAA GGAGTACGGC 
AGTGAAAAGA AGGGCTTCCT GCTGAAGAAG AGTGATGGGA TCCGGAAAGT GTGGCAGAGA 
CGGAAGTGTG CGGTCAAGAA CGGGATCCTG ACCATCTCCC ACGCAACTTC CAACAGGCAG 
CCTGCTAAGT TAAACCTCCT CACCTGCCAA GTGAAACCGA ATGCTGAGGA CAAGAAGTCT 
TTTGACCTGA TATCACATAA TCGAACATAT CACTTTCAAG CAGAAGATGA GCAGGATTAT 
ATAGCGTGGA TATCAGTACT GACGAATAGC AAAGAAGAGG CCCTAACCAT GGCCTTCCGT 
GGTGAGCAGA GCACAGGGGA GAATAGCCTG GAGGACCTTA CCAAAGCCAT CATCGAGGAT 
GTACAGCGGC TCCCTGGGAA TGACATCTGC TGTGACTGCG GCTCATCAGA ACCCACGTGG 
CTTTCAACCA ACTTGGGTAT TTTGACCTGT ATAGAATGTT CCGGAATCCA TAGGGAAATG 
GGGGTTCATA TTTCTCGCAT TCAGTCTTTG GAACTAGACA AATTAGGAAC TTCCGAACTC 
TTGCTGGCCA AGAATGTAGG AAACAATAGT TTTAATGATA TTATGGAAGC AAATTTACCC 
AGCCCTTCAC CAAAGCCCAC CCCTTCAAGT GATATGACCG TGCGTAAAGA GTACATCACT 
GCAAAGTATG TCGACCACCG CTTCTCCAGG AAGACCTGTG CCTCTTCCTC AGCAAAGCTG 
AACGAATTGC TTGAGGCCAT CAAGTCCAGG GACTTACTTG CATTAATCCA AGTCTATGCA 
GAGGGGGTGG AGTTAATGGA GCCACTGCTG GAACCTGGGC AGGAGCTCGG GGAGACAGCC 
CTTCATCTTG CCGTCAGGAC CGCAGACCAG ACATCTCTCC ATCTGGTTGA TTTCCTTGTA 
CAAAACTGTG GGAACCTGGA TAAGCAAACG TCTGTGGGGA ATACAGTTCT GCACTATTGC 
AGCATGTACG GCAAGCCGGA GTGTCTGAAG CTGCTGCTTA GGAGCAAGCC CACCGTGGAC 
ATCGTTAACC AGAATGGAGA AACTGCCTTG GATATAGCAA AGAGACTCAA AGCTACCCAG 
TGTGAAGATC TGCTTTCCCA GGCTAAATCT GGAAAGTTCA ACCCTCATGT CCACGTAGAG 
TATGAGTGGA ATCTTCGACA GGACGAGATG GATGAGAGCG ATGACGATCT GGATGACAAG 
CCAAGCCCGA TCAAAAAGGA ACGCTCGCCC AGACCACAGA GCTTCTGCCA CTCCTCCAGC 
ATCTCGCCAC AGGACAAGCT GGCACTGCCA GGGTTCAGCA CTCCACGGGA CAAGCAGCGG 
CTCTCCTACG GAGCCTTCAC CAACCAGATC TTCGCCTCTA CGAGCACAGA TTTGCCCACA 
TCACCCACCA GTGAGGCTCC CCCTTTGCCA CCTCGGAACG CCGGGAAAGG TAATGATGTG 
GGCCCATTAT CTTCAAGTAA GACGGCCAAC AAGTTTGAGG GGCTGTCTCA GCAAGCAAGC 
ACCAGTTCTG CTAAGACTGC CCTTGGCCCG AGAGTGCTTC CTAAACTACC TCAGAAAGTG 
GCACTAAGGA AGACGGAGAC CAGCCATCAT CTCTCCCTCG ACAGAACCAA CATCCCACCT 
GAGACTTTTC AGAAATCATC ACAGTTGACA GAGTTACCCC AAAAGCCACC ACTTGGAGAG 
CTGCCCCCGA AGCCTGTGGA ACTGGCCCCC AAGCCCCAAG TTGGAGAGCT GCCACCTAAG 
CCTGGAGAGC TACCCCCTAA GCCCCAATTA GGTGACCTGC CCCCCAAGCC ACAGCTCTCA 
GACTTACCTC CCAAGCCACA GATGAAGGAC CTGCCCCCTA AGCCGCAGCT GGGGGATCTG 
CTGGCAAAGT CCCAGGCTGG CGATGTCTCA GCCAAGGTGC AGCCACCCTC AGAGGTCACA 
CAGAGGTCAC ACACCGGGGA TCTGTCTCCA AATGTACAGT CCAGAGATGC CATCCAGAAG 
CAAGCATCTG AAGACTCCAA CGACCTCACA CCCACGCTGC CAGAGACACC CGTACCACTG 
CCGAGAAAAA TCAATACGGG GAAAAATAAA GTGAGGCGGG TGAAGACCAT TTATGACTGC 
CAGGCAGATA ATGACGACGA ACTCACATTT ATTGAGGGGG AAGTGATCAT TGTCACCGGG 
GAAGAGGACC AGGAGTGGTG GATCGGGCAT ATCGAAGGAC AGCCTGAAAG GAAGGGTGTC 
TTTCCAGTGT CCTTTGTCCA CATCCTGTCT GACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asap1 ( NM_010026.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034156.2 Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu05418
Clone ID Related Accession (Same CDS sequence)	NM_010026.3
Accession Version	NM_010026.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC094581.1, AK147689.1, DV041486.1, AA266485.1 and AC156573.3. On Feb 15, 2013 this sequence version replaced NM_010026.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC094581.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGAGATCTT CAGCCTCCCG GCTCTCCAGT TTTTCCTCCA GAGATTCACT ATGGAATCGG 
ATGCCGGACC AGATCTCCGT GTCCGAGTTC ATCGCCGAGA CCACCGAGGA CTACAACTCG 
CCCACCACGT CCAGCTTCAC CACGCGGCTG CACAACTGCA GGAACACCGT CACGCTGCTG 
GAGGAGGCTC TAGACCAAGA TAGAACAGCC CTACAGAAAG TGAAGAAATC TGTAAAAGCA 
ATATATAATT CCGGTCAAGA TCATGTACAA AATGAAGAAA ACTATGCACA AGTTCTGGAT 
AAGTTTGGAA GTAATTTTTT AAGCAGAGAC AACCCTGACC TTGGCACCGC TTTTGTCAAG 
TTTTCTACAC TCACAAAGGA ACTGTCCACG CTGCTGAAAA ATCTGCTCCA GGGTCTGAGC 
CACAATGTGA TCTTCACCTT GGATTCCTTG TTAAAAGGAG ACTTGAAGGG GGTCAAAGGA 
GATCTCAAGA AGCCATTTGA CAAAGCCTGG AAAGATTATG AGACGAAGTT TACAAAAATT 
GAGAAGGAGA AAAGAGAGCA CGCAAAACAG CACGGGATGA TCCGAACAGA GATAACAGGC 
GCTGAGATTG CAGAAGAAAT GGAAAAGGAA CGGCGGCTTT TTCAGCTCCA GATGTGTGAA 
TATCTCATTA AAGTAAATGA AATCAAGACC AAAAAGGGTG TGGACCTGCT GCAGAACCTT 
ATAAAGTATT ACCATGCACA GTGCAATTTC TTTCAGGATG GCTTGAAAAC GGCTGATAAA 
CTGAAACAGT ACATAGAAAA GCTGGCTGCT GACTTATATA ATATAAAGCA AACCCAAGAT 
GAAGAAAAAA AACAGCTAAC TGCACTCCGA GATCTAATAA AATCCTCTCT GCAACTTGAT 
CCGAAAGAAG TAGGTGGTTT ATATGTTGCC AGCAGGGCTA ACAGTTCTAG AAGAGACTCT 
CAGAGCCGGC AAGGTGGGTA CAGCATGCAT CAGCTCCAGG GCAACAAGGA GTACGGCAGT 
GAAAAGAAGG GCTTCCTGCT GAAGAAGAGT GATGGGATCC GGAAAGTGTG GCAGAGACGG 
AAGTGTGCGG TCAAGAACGG GATCCTGACC ATCTCCCACG CAACTTCCAA CAGGCAGCCT 
GCTAAGTTAA ACCTCCTCAC CTGCCAAGTG AAACCGAATG CTGAGGACAA GAAGTCTTTT 
GACCTGATAT CACATAATCG AACATATCAC TTTCAAGCAG AAGATGAGCA GGATTATATA 
GCGTGGATAT CAGTACTGAC GAATAGCAAA GAAGAGGCCC TAACCATGGC CTTCCGTGGT 
GAGCAGAGCA CAGGGGAGAA TAGCCTGGAG GACCTTACCA AAGCCATCAT CGAGGATGTA 
CAGCGGCTCC CTGGGAATGA CATCTGCTGT GACTGCGGCT CATCAGAACC CACGTGGCTT 
TCAACCAACT TGGGTATTTT GACCTGTATA GAATGTTCCG GAATCCATAG GGAAATGGGG 
GTTCATATTT CTCGCATTCA GTCTTTGGAA CTAGACAAAT TAGGAACTTC CGAACTCTTG 
CTGGCCAAGA ATGTAGGAAA CAATAGTTTT AATGATATTA TGGAAGCAAA TTTACCCAGC 
CCTTCACCAA AGCCCACCCC TTCAAGTGAT ATGACCGTGC GTAAAGAGTA CATCACTGCA 
AAGTATGTCG ACCACCGCTT CTCCAGGAAG ACCTGTGCCT CTTCCTCAGC AAAGCTGAAC 
GAATTGCTTG AGGCCATCAA GTCCAGGGAC TTACTTGCAT TAATCCAAGT CTATGCAGAG 
GGGGTGGAGT TAATGGAGCC ACTGCTGGAA CCTGGGCAGG AGCTCGGGGA GACAGCCCTT 
CATCTTGCCG TCAGGACCGC AGACCAGACA TCTCTCCATC TGGTTGATTT CCTTGTACAA 
AACTGTGGGA ACCTGGATAA GCAAACGTCT GTGGGGAATA CAGTTCTGCA CTATTGCAGC 
ATGTACGGCA AGCCGGAGTG TCTGAAGCTG CTGCTTAGGA GCAAGCCCAC CGTGGACATC 
GTTAACCAGA ATGGAGAAAC TGCCTTGGAT ATAGCAAAGA GACTCAAAGC TACCCAGTGT 
GAAGATCTGC TTTCCCAGGC TAAATCTGGA AAGTTCAACC CTCATGTCCA CGTAGAGTAT 
GAGTGGAATC TTCGACAGGA CGAGATGGAT GAGAGCGATG ACGATCTGGA TGACAAGCCA 
AGCCCGATCA AAAAGGAACG CTCGCCCAGA CCACAGAGCT TCTGCCACTC CTCCAGCATC 
TCGCCACAGG ACAAGCTGGC ACTGCCAGGG TTCAGCACTC CACGGGACAA GCAGCGGCTC 
TCCTACGGAG CCTTCACCAA CCAGATCTTC GCCTCTACGA GCACAGATTT GCCCACATCA 
CCCACCAGTG AGGCTCCCCC TTTGCCACCT CGGAACGCCG GGAAAGGTCC AACTGGCCCA 
CCTTCAACAC TCCCTCTAGG CACCCAGACC TCTAGTGGCA GCTCCACCCT ATCCAAGAAG 
AGGCCTCCTC CCCCACCACC AGGACACAAG AGAACCCTGT CTGACCCTCC CAGCCCACTA 
CCTCACGGGC CCCCAAACAA AGGCGCAATT CCTTGGGGTA ATGATGTGGG CCCATTATCT 
TCAAGTAAGA CGGCCAACAA GTTTGAGGGG CTGTCTCAGC AAGCAAGCAC CAGTTCTGCT 
AAGACTGCCC TTGGCCCGAG AGTGCTTCCT AAACTACCTC AGAAAGTGGC ACTAAGGAAG 
ACGGAGACCA GCCATCATCT CTCCCTCGAC AGAACCAACA TCCCACCTGA GACTTTTCAG 
AAATCATCAC AGTTGACAGA GTTACCCCAA AAGCCACCAC TTGGAGAGCT GCCCCCGAAG 
CCTGTGGAAC TGGCCCCCAA GCCCCAAGTT GGAGAGCTGC CACCTAAGCC TGGAGAGCTA 
CCCCCTAAGC CCCAATTAGG TGACCTGCCC CCCAAGCCAC AGCTCTCAGA CTTACCTCCC 
AAGCCACAGA TGAAGGACCT GCCCCCTAAG CCGCAGCTGG GGGATCTGCT GGCAAAGTCC 
CAGGCTGGCG ATGTCTCAGC CAAGGTGCAG CCACCCTCAG AGGTCACACA GAGGTCACAC 
ACCGGGGATC TGTCTCCAAA TGTACAGTCC AGAGATGCCA TCCAGAAGCA AGCATCTGAA 
GACTCCAACG ACCTCACACC CACGCTGCCA GAGACACCCG TACCACTGCC GAGAAAAATC 
AATACGGGGA AAAATAAAGT GAGGCGGGTG AAGACCATTT ATGACTGCCA GGCAGATAAT 
GACGACGAAC TCACATTTAT TGAGGGGGAA GTGATCATTG TCACCGGGGA AGAGGACCAG 
GAGTGGTGGA TCGGGCATAT CGAAGGACAG CCTGAAAGGA AGGGTGTCTT TCCAGTGTCC 
TTTGTCCACA TCCTGTCTGA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034156.2
CDS	231..3674
Translation	MRSSASRLSSFSSRDSLWNRMPDQISVSEFIAETTEDYNSPTTSSFTTRLHNCRNTVTLLEEALDQDRTALQKVKKSVKAIYNSGQDHVQNEENYAQVLDKFGSNFLSRDNPDLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGVKGDLKKPFDKAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDPKEVGGLYVASRANSSRRDSQSRQGGYSMHQLQGNKEYGSEKKGFLLKKSDGIRKVWQRRKCAVKNGILTISHATSNRQPAKLNLLTCQVKPNAEDKKSFDLISHNRTYHFQAEDEQDYIAWISVLTNSKEEALTMAFRGEQSTGENSLEDLTKAIIEDVQRLPGNDICCDCGSSEPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDKLGTSELLLAKNVGNNSFNDIMEANLPSPSPKPTPSSDMTVRKEYITAKYVDHRFSRKTCASSSAKLNELLEAIKSRDLLALIQVYAEGVELMEPLLEPGQELGETALHLAVRTADQTSLHLVDFLVQNCGNLDKQTSVGNTVLHYCSMYGKPECLKLLLRSKPTVDIVNQNGETALDIAKRLKATQCEDLLSQAKSGKFNPHVHVEYEWNLRQDEMDESDDDLDDKPSPIKKERSPRPQSFCHSSSISPQDKLALPGFSTPRDKQRLSYGAFTNQIFASTSTDLPTSPTSEAPPLPPRNAGKGPTGPPSTLPLGTQTSSGSSTLSKKRPPPPPPGHKRTLSDPPSPLPHGPPNKGAIPWGNDVGPLSSSKTANKFEGLSQQASTSSAKTALGPRVLPKLPQKVALRKTETSHHLSLDRTNIPPETFQKSSQLTELPQKPPLGELPPKPVELAPKPQVGELPPKPGELPPKPQLGDLPPKPQLSDLPPKPQMKDLPPKPQLGDLLAKSQAGDVSAKVQPPSEVTQRSHTGDLSPNVQSRDAIQKQASEDSNDLTPTLPETPVPLPRKINTGKNKVRRVKTIYDCQADNDDELTFIEGEVIIVTGEEDQEWWIGHIEGQPERKGVFPVSFVHILSD

Target ORF information:

RefSeq Version	NM_010026.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010026.3

ORF Insert Sequence:

ATGAGATCTT CAGCCTCCCG GCTCTCCAGT TTTTCCTCCA GAGATTCACT ATGGAATCGG 
ATGCCGGACC AGATCTCCGT GTCCGAGTTC ATCGCCGAGA CCACCGAGGA CTACAACTCG 
CCCACCACGT CCAGCTTCAC CACGCGGCTG CACAACTGCA GGAACACCGT CACGCTGCTG 
GAGGAGGCTC TAGACCAAGA TAGAACAGCC CTACAGAAAG TGAAGAAATC TGTAAAAGCA 
ATATATAATT CCGGTCAAGA TCATGTACAA AATGAAGAAA ACTATGCACA AGTTCTGGAT 
AAGTTTGGAA GTAATTTTTT AAGCAGAGAC AACCCTGACC TTGGCACCGC TTTTGTCAAG 
TTTTCTACAC TCACAAAGGA ACTGTCCACG CTGCTGAAAA ATCTGCTCCA GGGTCTGAGC 
CACAATGTGA TCTTCACCTT GGATTCCTTG TTAAAAGGAG ACTTGAAGGG GGTCAAAGGA 
GATCTCAAGA AGCCATTTGA CAAAGCCTGG AAAGATTATG AGACGAAGTT TACAAAAATT 
GAGAAGGAGA AAAGAGAGCA CGCAAAACAG CACGGGATGA TCCGAACAGA GATAACAGGC 
GCTGAGATTG CAGAAGAAAT GGAAAAGGAA CGGCGGCTTT TTCAGCTCCA GATGTGTGAA 
TATCTCATTA AAGTAAATGA AATCAAGACC AAAAAGGGTG TGGACCTGCT GCAGAACCTT 
ATAAAGTATT ACCATGCACA GTGCAATTTC TTTCAGGATG GCTTGAAAAC GGCTGATAAA 
CTGAAACAGT ACATAGAAAA GCTGGCTGCT GACTTATATA ATATAAAGCA AACCCAAGAT 
GAAGAAAAAA AACAGCTAAC TGCACTCCGA GATCTAATAA AATCCTCTCT GCAACTTGAT 
CCGAAAGAAG TAGGTGGTTT ATATGTTGCC AGCAGGGCTA ACAGTTCTAG AAGAGACTCT 
CAGAGCCGGC AAGGTGGGTA CAGCATGCAT CAGCTCCAGG GCAACAAGGA GTACGGCAGT 
GAAAAGAAGG GCTTCCTGCT GAAGAAGAGT GATGGGATCC GGAAAGTGTG GCAGAGACGG 
AAGTGTGCGG TCAAGAACGG GATCCTGACC ATCTCCCACG CAACTTCCAA CAGGCAGCCT 
GCTAAGTTAA ACCTCCTCAC CTGCCAAGTG AAACCGAATG CTGAGGACAA GAAGTCTTTT 
GACCTGATAT CACATAATCG AACATATCAC TTTCAAGCAG AAGATGAGCA GGATTATATA 
GCGTGGATAT CAGTACTGAC GAATAGCAAA GAAGAGGCCC TAACCATGGC CTTCCGTGGT 
GAGCAGAGCA CAGGGGAGAA TAGCCTGGAG GACCTTACCA AAGCCATCAT CGAGGATGTA 
CAGCGGCTCC CTGGGAATGA CATCTGCTGT GACTGCGGCT CATCAGAACC CACGTGGCTT 
TCAACCAACT TGGGTATTTT GACCTGTATA GAATGTTCCG GAATCCATAG GGAAATGGGG 
GTTCATATTT CTCGCATTCA GTCTTTGGAA CTAGACAAAT TAGGAACTTC CGAACTCTTG 
CTGGCCAAGA ATGTAGGAAA CAATAGTTTT AATGATATTA TGGAAGCAAA TTTACCCAGC 
CCTTCACCAA AGCCCACCCC TTCAAGTGAT ATGACCGTGC GTAAAGAGTA CATCACTGCA 
AAGTATGTCG ACCACCGCTT CTCCAGGAAG ACCTGTGCCT CTTCCTCAGC AAAGCTGAAC 
GAATTGCTTG AGGCCATCAA GTCCAGGGAC TTACTTGCAT TAATCCAAGT CTATGCAGAG 
GGGGTGGAGT TAATGGAGCC ACTGCTGGAA CCTGGGCAGG AGCTCGGGGA GACAGCCCTT 
CATCTTGCCG TCAGGACCGC AGACCAGACA TCTCTCCATC TGGTTGATTT CCTTGTACAA 
AACTGTGGGA ACCTGGATAA GCAAACGTCT GTGGGGAATA CAGTTCTGCA CTATTGCAGC 
ATGTACGGCA AGCCGGAGTG TCTGAAGCTG CTGCTTAGGA GCAAGCCCAC CGTGGACATC 
GTTAACCAGA ATGGAGAAAC TGCCTTGGAT ATAGCAAAGA GACTCAAAGC TACCCAGTGT 
GAAGATCTGC TTTCCCAGGC TAAATCTGGA AAGTTCAACC CTCATGTCCA CGTAGAGTAT 
GAGTGGAATC TTCGACAGGA CGAGATGGAT GAGAGCGATG ACGATCTGGA TGACAAGCCA 
AGCCCGATCA AAAAGGAACG CTCGCCCAGA CCACAGAGCT TCTGCCACTC CTCCAGCATC 
TCGCCACAGG ACAAGCTGGC ACTGCCAGGG TTCAGCACTC CACGGGACAA GCAGCGGCTC 
TCCTACGGAG CCTTCACCAA CCAGATCTTC GCCTCTACGA GCACAGATTT GCCCACATCA 
CCCACCAGTG AGGCTCCCCC TTTGCCACCT CGGAACGCCG GGAAAGGTCC AACTGGCCCA 
CCTTCAACAC TCCCTCTAGG CACCCAGACC TCTAGTGGCA GCTCCACCCT ATCCAAGAAG 
AGGCCTCCTC CCCCACCACC AGGACACAAG AGAACCCTGT CTGACCCTCC CAGCCCACTA 
CCTCACGGGC CCCCAAACAA AGGCGCAATT CCTTGGGGTA ATGATGTGGG CCCATTATCT 
TCAAGTAAGA CGGCCAACAA GTTTGAGGGG CTGTCTCAGC AAGCAAGCAC CAGTTCTGCT 
AAGACTGCCC TTGGCCCGAG AGTGCTTCCT AAACTACCTC AGAAAGTGGC ACTAAGGAAG 
ACGGAGACCA GCCATCATCT CTCCCTCGAC AGAACCAACA TCCCACCTGA GACTTTTCAG 
AAATCATCAC AGTTGACAGA GTTACCCCAA AAGCCACCAC TTGGAGAGCT GCCCCCGAAG 
CCTGTGGAAC TGGCCCCCAA GCCCCAAGTT GGAGAGCTGC CACCTAAGCC TGGAGAGCTA 
CCCCCTAAGC CCCAATTAGG TGACCTGCCC CCCAAGCCAC AGCTCTCAGA CTTACCTCCC 
AAGCCACAGA TGAAGGACCT GCCCCCTAAG CCGCAGCTGG GGGATCTGCT GGCAAAGTCC 
CAGGCTGGCG ATGTCTCAGC CAAGGTGCAG CCACCCTCAG AGGTCACACA GAGGTCACAC 
ACCGGGGATC TGTCTCCAAA TGTACAGTCC AGAGATGCCA TCCAGAAGCA AGCATCTGAA 
GACTCCAACG ACCTCACACC CACGCTGCCA GAGACACCCG TACCACTGCC GAGAAAAATC 
AATACGGGGA AAAATAAAGT GAGGCGGGTG AAGACCATTT ATGACTGCCA GGCAGATAAT 
GACGACGAAC TCACATTTAT TGAGGGGGAA GTGATCATTG TCACCGGGGA AGAGGACCAG 
GAGTGGTGGA TCGGGCATAT CGAAGGACAG CCTGAAAGGA AGGGTGTCTT TCCAGTGTCC 
TTTGTCCACA TCCTGTCTGA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asap1 ( NM_001276467.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001263396.1 Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OMu17132
Clone ID Related Accession (Same CDS sequence)	NM_001276467.1
Accession Version	NM_001276467.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform e
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CF735731.1, CB527210.1, CD355387.1, CF746306.1, DT921606.1, BG808597.1, BU707744.1, BC094581.1, AK147689.1, DV041486.1, AA266485.1 and AC156573.3. Transcript Variant: This variant (5) lacks an in-frame exon in the coding region, compared to variant 1. The encoded isoform (e) is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AF075462.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGAGATCTT CAGCCTCCCG GCTCTCCAGT TTTTCCTCCA GAGATTCACT ATGGAATCGG 
ATGCCGGACC AGATCTCCGT GTCCGAGTTC ATCGCCGAGA CCACCGAGGA CTACAACTCG 
CCCACCACGT CCAGCTTCAC CACGCGGCTG CACAACTGCA GGAACACCGT CACGCTGCTG 
GAGGAGGCTC TAGACCAAGA TAGAACAGCC CTACAGAAAG TGAAGAAATC TGTAAAAGCA 
ATATATAATT CCGGTCAAGA TCATGTACAA AATGAAGAAA ACTATGCACA AGTTCTGGAT 
AAGTTTGGAA GTAATTTTTT AAGCAGAGAC AACCCTGACC TTGGCACCGC TTTTGTCAAG 
TTTTCTACAC TCACAAAGGA ACTGTCCACG CTGCTGAAAA ATCTGCTCCA GGGTCTGAGC 
CACAATGTGA TCTTCACCTT GGATTCCTTG TTAAAAGGAG ACTTGAAGGG GGTCAAAGGA 
GATCTCAAGA AGCCATTTGA CAAAGCCTGG AAAGATTATG AGACGAAGTT TACAAAAATT 
GAGAAGGAGA AAAGAGAGCA CGCAAAACAG CACGGGATGA TCCGAACAGA GATAACAGGC 
GCTGAGATTG CAGAAGAAAT GGAAAAGGAA CGGCGGCTTT TTCAGCTCCA GATGTGTGAA 
TATCTCATTA AAGTAAATGA AATCAAGACC AAAAAGGGTG TGGACCTGCT GCAGAACCTT 
ATAAAGTATT ACCATGCACA GTGCAATTTC TTTCAGGATG GCTTGAAAAC GGCTGATAAA 
CTGAAACAGT ACATAGAAAA GCTGGCTGCT GACTTATATA ATATAAAGCA AACCCAAGAT 
GAAGAAAAAA AACAGCTAAC TGCACTCCGA GATCTAATAA AATCCTCTCT GCAACTTGAT 
CCGAAAGAAG TAGGTGGTTT ATATGTTGCC AGCAGGGCTA ACAGTTCTAG AAGAGACTCT 
CAGAGCCGGC AAGGTGGGTA CAGCATGCAT CAGCTCCAGG GCAACAAGGA GTACGGCAGT 
GAAAAGAAGG GCTTCCTGCT GAAGAAGAGT GATGGGATCC GGAAAGTGTG GCAGAGACGG 
AAGTGTGCGG TCAAGAACGG GATCCTGACC ATCTCCCACG CAACTTCCAA CAGGCAGCCT 
GCTAAGTTAA ACCTCCTCAC CTGCCAAGTG AAACCGAATG CTGAGGACAA GAAGTCTTTT 
GACCTGATAT CACATAATCG AACATATCAC TTTCAAGCAG AAGATGAGCA GGATTATATA 
GCGTGGATAT CAGTACTGAC GAATAGCAAA GAAGAGGCCC TAACCATGGC CTTCCGTGGT 
GAGCAGAGCA CAGGGGAGAA TAGCCTGGAG GACCTTACCA AAGCCATCAT CGAGGATGTA 
CAGCGGCTCC CTGGGAATGA CATCTGCTGT GACTGCGGCT CATCAGAACC CACGTGGCTT 
TCAACCAACT TGGGTATTTT GACCTGTATA GAATGTTCCG GAATCCATAG GGAAATGGGG 
GTTCATATTT CTCGCATTCA GTCTTTGGAA CTAGACAAAT TAGGAACTTC CGAACTCTTG 
CTGGCCAAGA ATGTAGGAAA CAATAGTTTT AATGATATTA TGGAAGCAAA TTTACCCAGC 
CCTTCACCAA AGCCCACCCC TTCAAGTGAT ATGACCGTGC GTAAAGAGTA CATCACTGCA 
AAGTATGTCG ACCACCGCTT CTCCAGGAAG ACCTGTGCCT CTTCCTCAGC AAAGCTGAAC 
GAATTGCTTG AGGCCATCAA GTCCAGGGAC TTACTTGCAT TAATCCAAGT CTATGCAGAG 
GGGGTGGAGT TAATGGAGCC ACTGCTGGAA CCTGGGCAGG AGCTCGGGGA GACAGCCCTT 
CATCTTGCCG TCAGGACCGC AGACCAGACA TCTCTCCATC TGGTTGATTT CCTTGTACAA 
AACTGTGGGA ACCTGGATAA GCAAACGTCT GTGGGGAATA CAGTTCTGCA CTATTGCAGC 
ATGTACGGCA AGCCGGAGTG TCTGAAGCTG CTGCTTAGGA GCAAGCCCAC CGTGGACATC 
GTTAACCAGA ATGGAGAAAC TGCCTTGGAT ATAGCAAAGA GACTCAAAGC TACCCAGTGT 
GAAGATCTGC TTTCCCAGGC TAAATCTGGA AAGTTCAACC CTCATGTCCA CGTAGAGTAT 
GAGTGGAATC TTCGACAGGA CGAGATGGAT GAGAGCGATG ACGATCTGGA TGACAAGCCA 
AGCCCGATCA AAAAGGAACG CTCGCCCAGA CCACAGAGCT TCTGCCACTC CTCCAGCATC 
TCGCCACAGG ACAAGCTGGC ACTGCCAGGG TTCAGCACTC CACGGGACAA GCAGCGGCTC 
TCCTACGGAG CCTTCACCAA CCAGATCTTC GCCTCTACGA GCACAGATTT GCCCACATCA 
CCCACCAGTG AGGCTCCCCC TTTGCCACCT CGGAACGCCG GGAAAGGTAA TGATGTGGGC 
CCATTATCTT CAAGTAAGAC GGCCAACAAG TTTGAGGGGC TGTCTCAGCA AGCAAGCACC 
AGTTCTGCTA AGACTGCCCT TGGCCCGAGA GTGCTTCCTA AACTACCTCA GAAAGTGGCA 
CTAAGGAAGA CGGAGACCAG CCATCATCTC TCCCTCGACA GAACCAACAT CCCACCTGAG 
ACTTTTCAGA AATCATCACA GTTGACAGAG TTACCCCAAA AGCCACCACT TGGAGAGCTG 
CCCCCGAAGC CTGTGGAACT GGCCCCCAAG CCCCAAGTTG GAGAGCTGCC ACCTAAGCCT 
GGAGAGCTAC CCCCTAAGCC CCAATTAGGT GACCTGCCCC CCAAGCCACA GCTCTCAGAC 
TTACCTCCCA AGCCACAGAT GAAGGACCTG CCCCCTAAGC CGCAGCTGGG GGATCTGCTG 
GCAAAGTCCC AGGCTGGCGA TGTCTCAGCC AAGGTGCAGC CACCCTCAGA GGTCACACAG 
AGGTCACACA CCGGGGATCT GTCTCCAAAT GTACAGTCCA GAGATGCCAT CCAGAAGCAA 
GCATCTGAAG ACTCCAACGA CCTCACACCC ACGCTGCCAG AGACACCCGT ACCACTGCCG 
AGAAAAATCA ATACGGGGAA AAATAAAGTG AGGCGGGTGA AGACCATTTA TGACTGCCAG 
GCAGATAATG ACGACGAACT CACATTTATT GAGGGGGAAG TGATCATTGT CACCGGGGAA 
GAGGACCAGG AGTGGTGGAT CGGGCATATC GAAGGACAGC CTGAAAGGAA GGGTGTCTTT 
CCAGTGTCCT TTGTCCACAT CCTGTCTGAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001263396.1
CDS	231..3503
Translation	MRSSASRLSSFSSRDSLWNRMPDQISVSEFIAETTEDYNSPTTSSFTTRLHNCRNTVTLLEEALDQDRTALQKVKKSVKAIYNSGQDHVQNEENYAQVLDKFGSNFLSRDNPDLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGVKGDLKKPFDKAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDPKEVGGLYVASRANSSRRDSQSRQGGYSMHQLQGNKEYGSEKKGFLLKKSDGIRKVWQRRKCAVKNGILTISHATSNRQPAKLNLLTCQVKPNAEDKKSFDLISHNRTYHFQAEDEQDYIAWISVLTNSKEEALTMAFRGEQSTGENSLEDLTKAIIEDVQRLPGNDICCDCGSSEPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDKLGTSELLLAKNVGNNSFNDIMEANLPSPSPKPTPSSDMTVRKEYITAKYVDHRFSRKTCASSSAKLNELLEAIKSRDLLALIQVYAEGVELMEPLLEPGQELGETALHLAVRTADQTSLHLVDFLVQNCGNLDKQTSVGNTVLHYCSMYGKPECLKLLLRSKPTVDIVNQNGETALDIAKRLKATQCEDLLSQAKSGKFNPHVHVEYEWNLRQDEMDESDDDLDDKPSPIKKERSPRPQSFCHSSSISPQDKLALPGFSTPRDKQRLSYGAFTNQIFASTSTDLPTSPTSEAPPLPPRNAGKGNDVGPLSSSKTANKFEGLSQQASTSSAKTALGPRVLPKLPQKVALRKTETSHHLSLDRTNIPPETFQKSSQLTELPQKPPLGELPPKPVELAPKPQVGELPPKPGELPPKPQLGDLPPKPQLSDLPPKPQMKDLPPKPQLGDLLAKSQAGDVSAKVQPPSEVTQRSHTGDLSPNVQSRDAIQKQASEDSNDLTPTLPETPVPLPRKINTGKNKVRRVKTIYDCQADNDDELTFIEGEVIIVTGEEDQEWWIGHIEGQPERKGVFPVSFVHILSD

Target ORF information:

RefSeq Version	NM_001276467.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001276467.1

ORF Insert Sequence:

ATGAGATCTT CAGCCTCCCG GCTCTCCAGT TTTTCCTCCA GAGATTCACT ATGGAATCGG 
ATGCCGGACC AGATCTCCGT GTCCGAGTTC ATCGCCGAGA CCACCGAGGA CTACAACTCG 
CCCACCACGT CCAGCTTCAC CACGCGGCTG CACAACTGCA GGAACACCGT CACGCTGCTG 
GAGGAGGCTC TAGACCAAGA TAGAACAGCC CTACAGAAAG TGAAGAAATC TGTAAAAGCA 
ATATATAATT CCGGTCAAGA TCATGTACAA AATGAAGAAA ACTATGCACA AGTTCTGGAT 
AAGTTTGGAA GTAATTTTTT AAGCAGAGAC AACCCTGACC TTGGCACCGC TTTTGTCAAG 
TTTTCTACAC TCACAAAGGA ACTGTCCACG CTGCTGAAAA ATCTGCTCCA GGGTCTGAGC 
CACAATGTGA TCTTCACCTT GGATTCCTTG TTAAAAGGAG ACTTGAAGGG GGTCAAAGGA 
GATCTCAAGA AGCCATTTGA CAAAGCCTGG AAAGATTATG AGACGAAGTT TACAAAAATT 
GAGAAGGAGA AAAGAGAGCA CGCAAAACAG CACGGGATGA TCCGAACAGA GATAACAGGC 
GCTGAGATTG CAGAAGAAAT GGAAAAGGAA CGGCGGCTTT TTCAGCTCCA GATGTGTGAA 
TATCTCATTA AAGTAAATGA AATCAAGACC AAAAAGGGTG TGGACCTGCT GCAGAACCTT 
ATAAAGTATT ACCATGCACA GTGCAATTTC TTTCAGGATG GCTTGAAAAC GGCTGATAAA 
CTGAAACAGT ACATAGAAAA GCTGGCTGCT GACTTATATA ATATAAAGCA AACCCAAGAT 
GAAGAAAAAA AACAGCTAAC TGCACTCCGA GATCTAATAA AATCCTCTCT GCAACTTGAT 
CCGAAAGAAG TAGGTGGTTT ATATGTTGCC AGCAGGGCTA ACAGTTCTAG AAGAGACTCT 
CAGAGCCGGC AAGGTGGGTA CAGCATGCAT CAGCTCCAGG GCAACAAGGA GTACGGCAGT 
GAAAAGAAGG GCTTCCTGCT GAAGAAGAGT GATGGGATCC GGAAAGTGTG GCAGAGACGG 
AAGTGTGCGG TCAAGAACGG GATCCTGACC ATCTCCCACG CAACTTCCAA CAGGCAGCCT 
GCTAAGTTAA ACCTCCTCAC CTGCCAAGTG AAACCGAATG CTGAGGACAA GAAGTCTTTT 
GACCTGATAT CACATAATCG AACATATCAC TTTCAAGCAG AAGATGAGCA GGATTATATA 
GCGTGGATAT CAGTACTGAC GAATAGCAAA GAAGAGGCCC TAACCATGGC CTTCCGTGGT 
GAGCAGAGCA CAGGGGAGAA TAGCCTGGAG GACCTTACCA AAGCCATCAT CGAGGATGTA 
CAGCGGCTCC CTGGGAATGA CATCTGCTGT GACTGCGGCT CATCAGAACC CACGTGGCTT 
TCAACCAACT TGGGTATTTT GACCTGTATA GAATGTTCCG GAATCCATAG GGAAATGGGG 
GTTCATATTT CTCGCATTCA GTCTTTGGAA CTAGACAAAT TAGGAACTTC CGAACTCTTG 
CTGGCCAAGA ATGTAGGAAA CAATAGTTTT AATGATATTA TGGAAGCAAA TTTACCCAGC 
CCTTCACCAA AGCCCACCCC TTCAAGTGAT ATGACCGTGC GTAAAGAGTA CATCACTGCA 
AAGTATGTCG ACCACCGCTT CTCCAGGAAG ACCTGTGCCT CTTCCTCAGC AAAGCTGAAC 
GAATTGCTTG AGGCCATCAA GTCCAGGGAC TTACTTGCAT TAATCCAAGT CTATGCAGAG 
GGGGTGGAGT TAATGGAGCC ACTGCTGGAA CCTGGGCAGG AGCTCGGGGA GACAGCCCTT 
CATCTTGCCG TCAGGACCGC AGACCAGACA TCTCTCCATC TGGTTGATTT CCTTGTACAA 
AACTGTGGGA ACCTGGATAA GCAAACGTCT GTGGGGAATA CAGTTCTGCA CTATTGCAGC 
ATGTACGGCA AGCCGGAGTG TCTGAAGCTG CTGCTTAGGA GCAAGCCCAC CGTGGACATC 
GTTAACCAGA ATGGAGAAAC TGCCTTGGAT ATAGCAAAGA GACTCAAAGC TACCCAGTGT 
GAAGATCTGC TTTCCCAGGC TAAATCTGGA AAGTTCAACC CTCATGTCCA CGTAGAGTAT 
GAGTGGAATC TTCGACAGGA CGAGATGGAT GAGAGCGATG ACGATCTGGA TGACAAGCCA 
AGCCCGATCA AAAAGGAACG CTCGCCCAGA CCACAGAGCT TCTGCCACTC CTCCAGCATC 
TCGCCACAGG ACAAGCTGGC ACTGCCAGGG TTCAGCACTC CACGGGACAA GCAGCGGCTC 
TCCTACGGAG CCTTCACCAA CCAGATCTTC GCCTCTACGA GCACAGATTT GCCCACATCA 
CCCACCAGTG AGGCTCCCCC TTTGCCACCT CGGAACGCCG GGAAAGGTAA TGATGTGGGC 
CCATTATCTT CAAGTAAGAC GGCCAACAAG TTTGAGGGGC TGTCTCAGCA AGCAAGCACC 
AGTTCTGCTA AGACTGCCCT TGGCCCGAGA GTGCTTCCTA AACTACCTCA GAAAGTGGCA 
CTAAGGAAGA CGGAGACCAG CCATCATCTC TCCCTCGACA GAACCAACAT CCCACCTGAG 
ACTTTTCAGA AATCATCACA GTTGACAGAG TTACCCCAAA AGCCACCACT TGGAGAGCTG 
CCCCCGAAGC CTGTGGAACT GGCCCCCAAG CCCCAAGTTG GAGAGCTGCC ACCTAAGCCT 
GGAGAGCTAC CCCCTAAGCC CCAATTAGGT GACCTGCCCC CCAAGCCACA GCTCTCAGAC 
TTACCTCCCA AGCCACAGAT GAAGGACCTG CCCCCTAAGC CGCAGCTGGG GGATCTGCTG 
GCAAAGTCCC AGGCTGGCGA TGTCTCAGCC AAGGTGCAGC CACCCTCAGA GGTCACACAG 
AGGTCACACA CCGGGGATCT GTCTCCAAAT GTACAGTCCA GAGATGCCAT CCAGAAGCAA 
GCATCTGAAG ACTCCAACGA CCTCACACCC ACGCTGCCAG AGACACCCGT ACCACTGCCG 
AGAAAAATCA ATACGGGGAA AAATAAAGTG AGGCGGGTGA AGACCATTTA TGACTGCCAG 
GCAGATAATG ACGACGAACT CACATTTATT GAGGGGGAAG TGATCATTGT CACCGGGGAA 
GAGGACCAGG AGTGGTGGAT CGGGCATATC GAAGGACAGC CTGAAAGGAA GGGTGTCTTT 
CCAGTGTCCT TTGTCCACAT CCTGTCTGAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asap1 ( NM_001276462.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001263391.1 Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu17150
Clone ID Related Accession (Same CDS sequence)	NM_001276462.1
Accession Version	NM_001276462.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3375bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform c
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK147689.1, BC094581.1, DV041486.1, AA266485.1 and AC156573.3. Transcript Variant: This variant (3) differs in the 5' UTR, uses a downstream start codon, and lacks an in-frame exon in the coding region, compared to variant 1. The encoded isoform (c) is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK147689.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGCCGGACC AGATCTCCGT GTCCGAGTTC ATCGCCGAGA CCACCGAGGA CTACAACTCG 
CCCACCACGT CCAGCTTCAC CACGCGGCTG CACAACTGCA GGAACACCGT CACGCTGCTG 
GAGGAGGCTC TAGACCAAGA TAGAACAGCC CTACAGAAAG TGAAGAAATC TGTAAAAGCA 
ATATATAATT CCGGTCAAGA TCATGTACAA AATGAAGAAA ACTATGCACA AGTTCTGGAT 
AAGTTTGGAA GTAATTTTTT AAGCAGAGAC AACCCTGACC TTGGCACCGC TTTTGTCAAG 
TTTTCTACAC TCACAAAGGA ACTGTCCACG CTGCTGAAAA ATCTGCTCCA GGGTCTGAGC 
CACAATGTGA TCTTCACCTT GGATTCCTTG TTAAAAGGAG ACTTGAAGGG GGTCAAAGGA 
GATCTCAAGA AGCCATTTGA CAAAGCCTGG AAAGATTATG AGACGAAGTT TACAAAAATT 
GAGAAGGAGA AAAGAGAGCA CGCAAAACAG CACGGGATGA TCCGAACAGA GATAACAGGC 
GCTGAGATTG CAGAAGAAAT GGAAAAGGAA CGGCGGCTTT TTCAGCTCCA GATGTGTGAA 
TATCTCATTA AAGTAAATGA AATCAAGACC AAAAAGGGTG TGGACCTGCT GCAGAACCTT 
ATAAAGTATT ACCATGCACA GTGCAATTTC TTTCAGGATG GCTTGAAAAC GGCTGATAAA 
CTGAAACAGT ACATAGAAAA GCTGGCTGCT GACTTATATA ATATAAAGCA AACCCAAGAT 
GAAGAAAAAA AACAGCTAAC TGCACTCCGA GATCTAATAA AATCCTCTCT GCAACTTGAT 
CCGAAAGAAG TAGGTGGTTT ATATGTTGCC AGCAGGGCTA ACAGTGACTC TCAGAGCCGG 
CAAGGTGGGT ACAGCATGCA TCAGCTCCAG GGCAACAAGG AGTACGGCAG TGAAAAGAAG 
GGCTTCCTGC TGAAGAAGAG TGATGGGATC CGGAAAGTGT GGCAGAGACG GAAGTGTGCG 
GTCAAGAACG GGATCCTGAC CATCTCCCAC GCAACTTCCA ACAGGCAGCC TGCTAAGTTA 
AACCTCCTCA CCTGCCAAGT GAAACCGAAT GCTGAGGACA AGAAGTCTTT TGACCTGATA 
TCACATAATC GAACATATCA CTTTCAAGCA GAAGATGAGC AGGATTATAT AGCGTGGATA 
TCAGTACTGA CGAATAGCAA AGAAGAGGCC CTAACCATGG CCTTCCGTGG TGAGCAGAGC 
ACAGGGGAGA ATAGCCTGGA GGACCTTACC AAAGCCATCA TCGAGGATGT ACAGCGGCTC 
CCTGGGAATG ACATCTGCTG TGACTGCGGC TCATCAGAAC CCACGTGGCT TTCAACCAAC 
TTGGGTATTT TGACCTGTAT AGAATGTTCC GGAATCCATA GGGAAATGGG GGTTCATATT 
TCTCGCATTC AGTCTTTGGA ACTAGACAAA TTAGGAACTT CCGAACTCTT GCTGGCCAAG 
AATGTAGGAA ACAATAGTTT TAATGATATT ATGGAAGCAA ATTTACCCAG CCCTTCACCA 
AAGCCCACCC CTTCAAGTGA TATGACCGTG CGTAAAGAGT ACATCACTGC AAAGTATGTC 
GACCACCGCT TCTCCAGGAA GACCTGTGCC TCTTCCTCAG CAAAGCTGAA CGAATTGCTT 
GAGGCCATCA AGTCCAGGGA CTTACTTGCA TTAATCCAAG TCTATGCAGA GGGGGTGGAG 
TTAATGGAGC CACTGCTGGA ACCTGGGCAG GAGCTCGGGG AGACAGCCCT TCATCTTGCC 
GTCAGGACCG CAGACCAGAC ATCTCTCCAT CTGGTTGATT TCCTTGTACA AAACTGTGGG 
AACCTGGATA AGCAAACGTC TGTGGGGAAT ACAGTTCTGC ACTATTGCAG CATGTACGGC 
AAGCCGGAGT GTCTGAAGCT GCTGCTTAGG AGCAAGCCCA CCGTGGACAT CGTTAACCAG 
AATGGAGAAA CTGCCTTGGA TATAGCAAAG AGACTCAAAG CTACCCAGTG TGAAGATCTG 
CTTTCCCAGG CTAAATCTGG AAAGTTCAAC CCTCATGTCC ACGTAGAGTA TGAGTGGAAT 
CTTCGACAGG ACGAGATGGA TGAGAGCGAT GACGATCTGG ATGACAAGCC AAGCCCGATC 
AAAAAGGAAC GCTCGCCCAG ACCACAGAGC TTCTGCCACT CCTCCAGCAT CTCGCCACAG 
GACAAGCTGG CACTGCCAGG GTTCAGCACT CCACGGGACA AGCAGCGGCT CTCCTACGGA 
GCCTTCACCA ACCAGATCTT CGCCTCTACG AGCACAGATT TGCCCACATC ACCCACCAGT 
GAGGCTCCCC CTTTGCCACC TCGGAACGCC GGGAAAGGTC CAACTGGCCC ACCTTCAACA 
CTCCCTCTAG GCACCCAGAC CTCTAGTGGC AGCTCCACCC TATCCAAGAA GAGGCCTCCT 
CCCCCACCAC CAGGACACAA GAGAACCCTG TCTGACCCTC CCAGCCCACT ACCTCACGGG 
CCCCCAAACA AAGGCGCAAT TCCTTGGGGT AATGATGTGG GCCCATTATC TTCAAGTAAG 
ACGGCCAACA AGTTTGAGGG GCTGTCTCAG CAAGCAAGCA CCAGTTCTGC TAAGACTGCC 
CTTGGCCCGA GAGTGCTTCC TAAACTACCT CAGAAAGTGG CACTAAGGAA GACGGAGACC 
AGCCATCATC TCTCCCTCGA CAGAACCAAC ATCCCACCTG AGACTTTTCA GAAATCATCA 
CAGTTGACAG AGTTACCCCA AAAGCCACCA CTTGGAGAGC TGCCCCCGAA GCCTGTGGAA 
CTGGCCCCCA AGCCCCAAGT TGGAGAGCTG CCACCTAAGC CTGGAGAGCT ACCCCCTAAG 
CCCCAATTAG GTGACCTGCC CCCCAAGCCA CAGCTCTCAG ACTTACCTCC CAAGCCACAG 
ATGAAGGACC TGCCCCCTAA GCCGCAGCTG GGGGATCTGC TGGCAAAGTC CCAGGCTGGC 
GATGTCTCAG CCAAGGTGCA GCCACCCTCA GAGGTCACAC AGAGGTCACA CACCGGGGAT 
CTGTCTCCAA ATGTACAGTC CAGAGATGCC ATCCAGAAGC AAGCATCTGA AGACTCCAAC 
GACCTCACAC CCACGCTGCC AGAGACACCC GTACCACTGC CGAGAAAAAT CAATACGGGG 
AAAAATAAAG TGAGGCGGGT GAAGACCATT TATGACTGCC AGGCAGATAA TGACGACGAA 
CTCACATTTA TTGAGGGGGA AGTGATCATT GTCACCGGGG AAGAGGACCA GGAGTGGTGG 
ATCGGGCATA TCGAAGGACA GCCTGAAAGG AAGGGTGTCT TTCCAGTGTC CTTTGTCCAC 
ATCCTGTCTG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001263391.1
CDS	200..3574
Translation	MPDQISVSEFIAETTEDYNSPTTSSFTTRLHNCRNTVTLLEEALDQDRTALQKVKKSVKAIYNSGQDHVQNEENYAQVLDKFGSNFLSRDNPDLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGVKGDLKKPFDKAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDPKEVGGLYVASRANSDSQSRQGGYSMHQLQGNKEYGSEKKGFLLKKSDGIRKVWQRRKCAVKNGILTISHATSNRQPAKLNLLTCQVKPNAEDKKSFDLISHNRTYHFQAEDEQDYIAWISVLTNSKEEALTMAFRGEQSTGENSLEDLTKAIIEDVQRLPGNDICCDCGSSEPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDKLGTSELLLAKNVGNNSFNDIMEANLPSPSPKPTPSSDMTVRKEYITAKYVDHRFSRKTCASSSAKLNELLEAIKSRDLLALIQVYAEGVELMEPLLEPGQELGETALHLAVRTADQTSLHLVDFLVQNCGNLDKQTSVGNTVLHYCSMYGKPECLKLLLRSKPTVDIVNQNGETALDIAKRLKATQCEDLLSQAKSGKFNPHVHVEYEWNLRQDEMDESDDDLDDKPSPIKKERSPRPQSFCHSSSISPQDKLALPGFSTPRDKQRLSYGAFTNQIFASTSTDLPTSPTSEAPPLPPRNAGKGPTGPPSTLPLGTQTSSGSSTLSKKRPPPPPPGHKRTLSDPPSPLPHGPPNKGAIPWGNDVGPLSSSKTANKFEGLSQQASTSSAKTALGPRVLPKLPQKVALRKTETSHHLSLDRTNIPPETFQKSSQLTELPQKPPLGELPPKPVELAPKPQVGELPPKPGELPPKPQLGDLPPKPQLSDLPPKPQMKDLPPKPQLGDLLAKSQAGDVSAKVQPPSEVTQRSHTGDLSPNVQSRDAIQKQASEDSNDLTPTLPETPVPLPRKINTGKNKVRRVKTIYDCQADNDDELTFIEGEVIIVTGEEDQEWWIGHIEGQPERKGVFPVSFVHILSD

Target ORF information:

RefSeq Version	NM_001276462.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001276462.1

ORF Insert Sequence:

ATGCCGGACC AGATCTCCGT GTCCGAGTTC ATCGCCGAGA CCACCGAGGA CTACAACTCG 
CCCACCACGT CCAGCTTCAC CACGCGGCTG CACAACTGCA GGAACACCGT CACGCTGCTG 
GAGGAGGCTC TAGACCAAGA TAGAACAGCC CTACAGAAAG TGAAGAAATC TGTAAAAGCA 
ATATATAATT CCGGTCAAGA TCATGTACAA AATGAAGAAA ACTATGCACA AGTTCTGGAT 
AAGTTTGGAA GTAATTTTTT AAGCAGAGAC AACCCTGACC TTGGCACCGC TTTTGTCAAG 
TTTTCTACAC TCACAAAGGA ACTGTCCACG CTGCTGAAAA ATCTGCTCCA GGGTCTGAGC 
CACAATGTGA TCTTCACCTT GGATTCCTTG TTAAAAGGAG ACTTGAAGGG GGTCAAAGGA 
GATCTCAAGA AGCCATTTGA CAAAGCCTGG AAAGATTATG AGACGAAGTT TACAAAAATT 
GAGAAGGAGA AAAGAGAGCA CGCAAAACAG CACGGGATGA TCCGAACAGA GATAACAGGC 
GCTGAGATTG CAGAAGAAAT GGAAAAGGAA CGGCGGCTTT TTCAGCTCCA GATGTGTGAA 
TATCTCATTA AAGTAAATGA AATCAAGACC AAAAAGGGTG TGGACCTGCT GCAGAACCTT 
ATAAAGTATT ACCATGCACA GTGCAATTTC TTTCAGGATG GCTTGAAAAC GGCTGATAAA 
CTGAAACAGT ACATAGAAAA GCTGGCTGCT GACTTATATA ATATAAAGCA AACCCAAGAT 
GAAGAAAAAA AACAGCTAAC TGCACTCCGA GATCTAATAA AATCCTCTCT GCAACTTGAT 
CCGAAAGAAG TAGGTGGTTT ATATGTTGCC AGCAGGGCTA ACAGTGACTC TCAGAGCCGG 
CAAGGTGGGT ACAGCATGCA TCAGCTCCAG GGCAACAAGG AGTACGGCAG TGAAAAGAAG 
GGCTTCCTGC TGAAGAAGAG TGATGGGATC CGGAAAGTGT GGCAGAGACG GAAGTGTGCG 
GTCAAGAACG GGATCCTGAC CATCTCCCAC GCAACTTCCA ACAGGCAGCC TGCTAAGTTA 
AACCTCCTCA CCTGCCAAGT GAAACCGAAT GCTGAGGACA AGAAGTCTTT TGACCTGATA 
TCACATAATC GAACATATCA CTTTCAAGCA GAAGATGAGC AGGATTATAT AGCGTGGATA 
TCAGTACTGA CGAATAGCAA AGAAGAGGCC CTAACCATGG CCTTCCGTGG TGAGCAGAGC 
ACAGGGGAGA ATAGCCTGGA GGACCTTACC AAAGCCATCA TCGAGGATGT ACAGCGGCTC 
CCTGGGAATG ACATCTGCTG TGACTGCGGC TCATCAGAAC CCACGTGGCT TTCAACCAAC 
TTGGGTATTT TGACCTGTAT AGAATGTTCC GGAATCCATA GGGAAATGGG GGTTCATATT 
TCTCGCATTC AGTCTTTGGA ACTAGACAAA TTAGGAACTT CCGAACTCTT GCTGGCCAAG 
AATGTAGGAA ACAATAGTTT TAATGATATT ATGGAAGCAA ATTTACCCAG CCCTTCACCA 
AAGCCCACCC CTTCAAGTGA TATGACCGTG CGTAAAGAGT ACATCACTGC AAAGTATGTC 
GACCACCGCT TCTCCAGGAA GACCTGTGCC TCTTCCTCAG CAAAGCTGAA CGAATTGCTT 
GAGGCCATCA AGTCCAGGGA CTTACTTGCA TTAATCCAAG TCTATGCAGA GGGGGTGGAG 
TTAATGGAGC CACTGCTGGA ACCTGGGCAG GAGCTCGGGG AGACAGCCCT TCATCTTGCC 
GTCAGGACCG CAGACCAGAC ATCTCTCCAT CTGGTTGATT TCCTTGTACA AAACTGTGGG 
AACCTGGATA AGCAAACGTC TGTGGGGAAT ACAGTTCTGC ACTATTGCAG CATGTACGGC 
AAGCCGGAGT GTCTGAAGCT GCTGCTTAGG AGCAAGCCCA CCGTGGACAT CGTTAACCAG 
AATGGAGAAA CTGCCTTGGA TATAGCAAAG AGACTCAAAG CTACCCAGTG TGAAGATCTG 
CTTTCCCAGG CTAAATCTGG AAAGTTCAAC CCTCATGTCC ACGTAGAGTA TGAGTGGAAT 
CTTCGACAGG ACGAGATGGA TGAGAGCGAT GACGATCTGG ATGACAAGCC AAGCCCGATC 
AAAAAGGAAC GCTCGCCCAG ACCACAGAGC TTCTGCCACT CCTCCAGCAT CTCGCCACAG 
GACAAGCTGG CACTGCCAGG GTTCAGCACT CCACGGGACA AGCAGCGGCT CTCCTACGGA 
GCCTTCACCA ACCAGATCTT CGCCTCTACG AGCACAGATT TGCCCACATC ACCCACCAGT 
GAGGCTCCCC CTTTGCCACC TCGGAACGCC GGGAAAGGTC CAACTGGCCC ACCTTCAACA 
CTCCCTCTAG GCACCCAGAC CTCTAGTGGC AGCTCCACCC TATCCAAGAA GAGGCCTCCT 
CCCCCACCAC CAGGACACAA GAGAACCCTG TCTGACCCTC CCAGCCCACT ACCTCACGGG 
CCCCCAAACA AAGGCGCAAT TCCTTGGGGT AATGATGTGG GCCCATTATC TTCAAGTAAG 
ACGGCCAACA AGTTTGAGGG GCTGTCTCAG CAAGCAAGCA CCAGTTCTGC TAAGACTGCC 
CTTGGCCCGA GAGTGCTTCC TAAACTACCT CAGAAAGTGG CACTAAGGAA GACGGAGACC 
AGCCATCATC TCTCCCTCGA CAGAACCAAC ATCCCACCTG AGACTTTTCA GAAATCATCA 
CAGTTGACAG AGTTACCCCA AAAGCCACCA CTTGGAGAGC TGCCCCCGAA GCCTGTGGAA 
CTGGCCCCCA AGCCCCAAGT TGGAGAGCTG CCACCTAAGC CTGGAGAGCT ACCCCCTAAG 
CCCCAATTAG GTGACCTGCC CCCCAAGCCA CAGCTCTCAG ACTTACCTCC CAAGCCACAG 
ATGAAGGACC TGCCCCCTAA GCCGCAGCTG GGGGATCTGC TGGCAAAGTC CCAGGCTGGC 
GATGTCTCAG CCAAGGTGCA GCCACCCTCA GAGGTCACAC AGAGGTCACA CACCGGGGAT 
CTGTCTCCAA ATGTACAGTC CAGAGATGCC ATCCAGAAGC AAGCATCTGA AGACTCCAAC 
GACCTCACAC CCACGCTGCC AGAGACACCC GTACCACTGC CGAGAAAAAT CAATACGGGG 
AAAAATAAAG TGAGGCGGGT GAAGACCATT TATGACTGCC AGGCAGATAA TGACGACGAA 
CTCACATTTA TTGAGGGGGA AGTGATCATT GTCACCGGGG AAGAGGACCA GGAGTGGTGG 
ATCGGGCATA TCGAAGGACA GCCTGAAAGG AAGGGTGTCT TTCCAGTGTC CTTTGTCCAC 
ATCCTGTCTG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asap1 ( NM_001276463.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001263392.1 Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant 4, mRNA.

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CloneID	OMu17242
Clone ID Related Accession (Same CDS sequence)	NM_001276463.1
Accession Version	NM_001276463.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3339bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform d
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK147689.1, AK132979.1, AC156573.3, CN455356.1, CN528328.1, CX768097.1, CN664630.1, CF175025.1, CO430667.1, BF470337.1, AK159048.1, DV041486.1 and AA266485.1. Transcript Variant: This variant (4) differs in the 5' UTR, uses a downstream start codon, and has multiple differences in the coding region but maintains the reading frame, compared to variant 1. The encoded isoform (d) is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC048818.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGCCGGACC AGATCTCCGT GTCCGAGTTC ATCGCCGAGA CCACCGAGGA CTACAACTCG 
CCCACCACGT CCAGCTTCAC CACGCGGCTG CACAACTGCA GGAACACCGT CACGCTGCTG 
GAGGAGGCTC TAGACCAAGA TAGAACAGCC CTACAGAAAG TGAAGAAATC TGTAAAAGCA 
ATATATAATT CCGGTCAAGA TCATGTACAA AATGAAGAAA ACTATGCACA AGTTCTGGAT 
AAGTTTGGAA GTAATTTTTT AAGCAGAGAC AACCCTGACC TTGGCACCGC TTTTGTCAAG 
TTTTCTACAC TCACAAAGGA ACTGTCCACG CTGCTGAAAA ATCTGCTCCA GGGTCTGAGC 
CACAATGTGA TCTTCACCTT GGATTCCTTG TTAAAAGGAG ACTTGAAGGG GGTCAAAGGA 
GATCTCAAGA AGCCATTTGA CAAAGCCTGG AAAGATTATG AGACGAAGTT TACAAAAATT 
GAGAAGGAGA AAAGAGAGCA CGCAAAACAG CACGGGATGA TCCGAACAGA GATAACAGGC 
GCTGAGATTG CAGAAGAAAT GGAAAAGGAA CGGCGGCTTT TTCAGCTCCA GATGTGTGAA 
TATCTCATTA AAGTAAATGA AATCAAGACC AAAAAGGGTG TGGACCTGCT GCAGAACCTT 
ATAAAGTATT ACCATGCACA GTGCAATTTC TTTCAGGATG GCTTGAAAAC GGCTGATAAA 
CTGAAACAGT ACATAGAAAA GCTGGCTGCT GACTTATATA ATATAAAGCA AACCCAAGAT 
GAAGAAAAAA AACAGCTAAC TGCACTCCGA GATCTAATAA AATCCTCTCT GCAACTTGAT 
CCGAAAGAAG ACTCTCAGAG CCGGCAAGGT GGGTACAGCA TGCATCAGCT CCAGGGCAAC 
AAGGAGTACG GCAGTGAAAA GAAGGGCTTC CTGCTGAAGA AGAGTGATGG GATCCGGAAA 
GTGTGGCAGA GACGGAAGTG TGCGGTCAAG AACGGGATCC TGACCATCTC CCACGCAACT 
TCCAACAGGC AGCCTGCTAA GTTAAACCTC CTCACCTGCC AAGTGAAACC GAATGCTGAG 
GACAAGAAGT CTTTTGACCT GATATCACAT AATCGAACAT ATCACTTTCA AGCAGAAGAT 
GAGCAGGATT ATATAGCGTG GATATCAGTA CTGACGAATA GCAAAGAAGA GGCCCTAACC 
ATGGCCTTCC GTGGTGAGCA GAGCACAGGG GAGAATAGCC TGGAGGACCT TACCAAAGCC 
ATCATCGAGG ATGTACAGCG GCTCCCTGGG AATGACATCT GCTGTGACTG CGGCTCATCA 
GAACCCACGT GGCTTTCAAC CAACTTGGGT ATTTTGACCT GTATAGAATG TTCCGGAATC 
CATAGGGAAA TGGGGGTTCA TATTTCTCGC ATTCAGTCTT TGGAACTAGA CAAATTAGGA 
ACTTCCGAAC TCTTGCTGGC CAAGAATGTA GGAAACAATA GTTTTAATGA TATTATGGAA 
GCAAATTTAC CCAGCCCTTC ACCAAAGCCC ACCCCTTCAA GTGATATGAC CGTGCGTAAA 
GAGTACATCA CTGCAAAGTA TGTCGACCAC CGCTTCTCCA GGAAGACCTG TGCCTCTTCC 
TCAGCAAAGC TGAACGAATT GCTTGAGGCC ATCAAGTCCA GGGACTTACT TGCATTAATC 
CAAGTCTATG CAGAGGGGGT GGAGTTAATG GAGCCACTGC TGGAACCTGG GCAGGAGCTC 
GGGGAGACAG CCCTTCATCT TGCCGTCAGG ACCGCAGACC AGACATCTCT CCATCTGGTT 
GATTTCCTTG TACAAAACTG TGGGAACCTG GATAAGCAAA CGTCTGTGGG GAATACAGTT 
CTGCACTATT GCAGCATGTA CGGCAAGCCG GAGTGTCTGA AGCTGCTGCT TAGGAGCAAG 
CCCACCGTGG ACATCGTTAA CCAGAATGGA GAAACTGCCT TGGATATAGC AAAGAGACTC 
AAAGCTACCC AGTGTGAAGA TCTGCTTTCC CAGGCTAAAT CTGGAAAGTT CAACCCTCAT 
GTCCACGTAG AGTATGAGTG GAATCTTCGA CAGGACGAGA TGGATGAGAG CGATGACGAT 
CTGGATGACA AGCCAAGCCC GATCAAAAAG GAACGCTCGC CCAGACCACA GAGCTTCTGC 
CACTCCTCCA GCATCTCGCC ACAGGACAAG CTGGCACTGC CAGGGTTCAG CACTCCACGG 
GACAAGCAGC GGCTCTCCTA CGGAGCCTTC ACCAACCAGA TCTTCGCCTC TACGAGCACA 
GATTTGCCCA CATCACCCAC CAGTGAGGCT CCCCCTTTGC CACCTCGGAA CGCCGGGAAA 
GGTCCAACTG GCCCACCTTC AACACTCCCT CTAGGCACCC AGACCTCTAG TGGCAGCTCC 
ACCCTATCCA AGAAGAGGCC TCCTCCCCCA CCACCAGGAC ACAAGAGAAC CCTGTCTGAC 
CCTCCCAGCC CACTACCTCA CGGGCCCCCA AACAAAGGCG CAATTCCTTG GGGTAATGAT 
GTGGGCCCAT TATCTTCAAG TAAGACGGCC AACAAGTTTG AGGGGCTGTC TCAGCAAGCA 
AGCACCAGTT CTGCTAAGAC TGCCCTTGGC CCGAGAGTGC TTCCTAAACT ACCTCAGAAA 
GTGGCACTAA GGAAGACGGA GACCAGCCAT CATCTCTCCC TCGACAGAAC CAACATCCCA 
CCTGAGACTT TTCAGAAATC ATCACAGTTG ACAGAGTTAC CCCAAAAGCC ACCACTTGGA 
GAGCTGCCCC CGAAGCCTGT GGAACTGGCC CCCAAGCCCC AAGTTGGAGA GCTGCCACCT 
AAGCCTGGAG AGCTACCCCC TAAGCCCCAA TTAGGTGACC TGCCCCCCAA GCCACAGCTC 
TCAGACTTAC CTCCCAAGCC ACAGATGAAG GACCTGCCCC CTAAGCCGCA GCTGGGGGAT 
CTGCTGGCAA AGTCCCAGGC TGGCGATGTC TCAGCCAAGG TGCAGCCACC CTCAGAGGTC 
ACACAGAGGT CACACACCGG GGATCTGTCT CCAAATGTAC AGTCCAGAGA TGCCATCCAG 
AAGCAAGCAT CTGAAGACTC CAACGACCTC ACACCCACGC TGCCAGAGAC ACCCGTACCA 
CTGCCGAGAA AAATCAATAC GGGGAAAAAT AAAGTGAGGC GGGTGAAGAC CATTTATGAC 
TGCCAGGCAG ATAATGACGA CGAACTCACA TTTATTGAGG GGGAAGTGAT CATTGTCACC 
GGGGAAGAGG ACCAGGAGTG GTGGATCGGG CATATCGAAG GACAGCCTGA AAGGAAGGGT 
GTCTTTCCAG TGTCCTTTGT CCACATCCTG TCTGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001263392.1
CDS	200..3538
Translation	MPDQISVSEFIAETTEDYNSPTTSSFTTRLHNCRNTVTLLEEALDQDRTALQKVKKSVKAIYNSGQDHVQNEENYAQVLDKFGSNFLSRDNPDLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGVKGDLKKPFDKAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDPKEDSQSRQGGYSMHQLQGNKEYGSEKKGFLLKKSDGIRKVWQRRKCAVKNGILTISHATSNRQPAKLNLLTCQVKPNAEDKKSFDLISHNRTYHFQAEDEQDYIAWISVLTNSKEEALTMAFRGEQSTGENSLEDLTKAIIEDVQRLPGNDICCDCGSSEPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDKLGTSELLLAKNVGNNSFNDIMEANLPSPSPKPTPSSDMTVRKEYITAKYVDHRFSRKTCASSSAKLNELLEAIKSRDLLALIQVYAEGVELMEPLLEPGQELGETALHLAVRTADQTSLHLVDFLVQNCGNLDKQTSVGNTVLHYCSMYGKPECLKLLLRSKPTVDIVNQNGETALDIAKRLKATQCEDLLSQAKSGKFNPHVHVEYEWNLRQDEMDESDDDLDDKPSPIKKERSPRPQSFCHSSSISPQDKLALPGFSTPRDKQRLSYGAFTNQIFASTSTDLPTSPTSEAPPLPPRNAGKGPTGPPSTLPLGTQTSSGSSTLSKKRPPPPPPGHKRTLSDPPSPLPHGPPNKGAIPWGNDVGPLSSSKTANKFEGLSQQASTSSAKTALGPRVLPKLPQKVALRKTETSHHLSLDRTNIPPETFQKSSQLTELPQKPPLGELPPKPVELAPKPQVGELPPKPGELPPKPQLGDLPPKPQLSDLPPKPQMKDLPPKPQLGDLLAKSQAGDVSAKVQPPSEVTQRSHTGDLSPNVQSRDAIQKQASEDSNDLTPTLPETPVPLPRKINTGKNKVRRVKTIYDCQADNDDELTFIEGEVIIVTGEEDQEWWIGHIEGQPERKGVFPVSFVHILSD

Target ORF information:

RefSeq Version	NM_001276463.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001276463.1

ORF Insert Sequence:

ATGCCGGACC AGATCTCCGT GTCCGAGTTC ATCGCCGAGA CCACCGAGGA CTACAACTCG 
CCCACCACGT CCAGCTTCAC CACGCGGCTG CACAACTGCA GGAACACCGT CACGCTGCTG 
GAGGAGGCTC TAGACCAAGA TAGAACAGCC CTACAGAAAG TGAAGAAATC TGTAAAAGCA 
ATATATAATT CCGGTCAAGA TCATGTACAA AATGAAGAAA ACTATGCACA AGTTCTGGAT 
AAGTTTGGAA GTAATTTTTT AAGCAGAGAC AACCCTGACC TTGGCACCGC TTTTGTCAAG 
TTTTCTACAC TCACAAAGGA ACTGTCCACG CTGCTGAAAA ATCTGCTCCA GGGTCTGAGC 
CACAATGTGA TCTTCACCTT GGATTCCTTG TTAAAAGGAG ACTTGAAGGG GGTCAAAGGA 
GATCTCAAGA AGCCATTTGA CAAAGCCTGG AAAGATTATG AGACGAAGTT TACAAAAATT 
GAGAAGGAGA AAAGAGAGCA CGCAAAACAG CACGGGATGA TCCGAACAGA GATAACAGGC 
GCTGAGATTG CAGAAGAAAT GGAAAAGGAA CGGCGGCTTT TTCAGCTCCA GATGTGTGAA 
TATCTCATTA AAGTAAATGA AATCAAGACC AAAAAGGGTG TGGACCTGCT GCAGAACCTT 
ATAAAGTATT ACCATGCACA GTGCAATTTC TTTCAGGATG GCTTGAAAAC GGCTGATAAA 
CTGAAACAGT ACATAGAAAA GCTGGCTGCT GACTTATATA ATATAAAGCA AACCCAAGAT 
GAAGAAAAAA AACAGCTAAC TGCACTCCGA GATCTAATAA AATCCTCTCT GCAACTTGAT 
CCGAAAGAAG ACTCTCAGAG CCGGCAAGGT GGGTACAGCA TGCATCAGCT CCAGGGCAAC 
AAGGAGTACG GCAGTGAAAA GAAGGGCTTC CTGCTGAAGA AGAGTGATGG GATCCGGAAA 
GTGTGGCAGA GACGGAAGTG TGCGGTCAAG AACGGGATCC TGACCATCTC CCACGCAACT 
TCCAACAGGC AGCCTGCTAA GTTAAACCTC CTCACCTGCC AAGTGAAACC GAATGCTGAG 
GACAAGAAGT CTTTTGACCT GATATCACAT AATCGAACAT ATCACTTTCA AGCAGAAGAT 
GAGCAGGATT ATATAGCGTG GATATCAGTA CTGACGAATA GCAAAGAAGA GGCCCTAACC 
ATGGCCTTCC GTGGTGAGCA GAGCACAGGG GAGAATAGCC TGGAGGACCT TACCAAAGCC 
ATCATCGAGG ATGTACAGCG GCTCCCTGGG AATGACATCT GCTGTGACTG CGGCTCATCA 
GAACCCACGT GGCTTTCAAC CAACTTGGGT ATTTTGACCT GTATAGAATG TTCCGGAATC 
CATAGGGAAA TGGGGGTTCA TATTTCTCGC ATTCAGTCTT TGGAACTAGA CAAATTAGGA 
ACTTCCGAAC TCTTGCTGGC CAAGAATGTA GGAAACAATA GTTTTAATGA TATTATGGAA 
GCAAATTTAC CCAGCCCTTC ACCAAAGCCC ACCCCTTCAA GTGATATGAC CGTGCGTAAA 
GAGTACATCA CTGCAAAGTA TGTCGACCAC CGCTTCTCCA GGAAGACCTG TGCCTCTTCC 
TCAGCAAAGC TGAACGAATT GCTTGAGGCC ATCAAGTCCA GGGACTTACT TGCATTAATC 
CAAGTCTATG CAGAGGGGGT GGAGTTAATG GAGCCACTGC TGGAACCTGG GCAGGAGCTC 
GGGGAGACAG CCCTTCATCT TGCCGTCAGG ACCGCAGACC AGACATCTCT CCATCTGGTT 
GATTTCCTTG TACAAAACTG TGGGAACCTG GATAAGCAAA CGTCTGTGGG GAATACAGTT 
CTGCACTATT GCAGCATGTA CGGCAAGCCG GAGTGTCTGA AGCTGCTGCT TAGGAGCAAG 
CCCACCGTGG ACATCGTTAA CCAGAATGGA GAAACTGCCT TGGATATAGC AAAGAGACTC 
AAAGCTACCC AGTGTGAAGA TCTGCTTTCC CAGGCTAAAT CTGGAAAGTT CAACCCTCAT 
GTCCACGTAG AGTATGAGTG GAATCTTCGA CAGGACGAGA TGGATGAGAG CGATGACGAT 
CTGGATGACA AGCCAAGCCC GATCAAAAAG GAACGCTCGC CCAGACCACA GAGCTTCTGC 
CACTCCTCCA GCATCTCGCC ACAGGACAAG CTGGCACTGC CAGGGTTCAG CACTCCACGG 
GACAAGCAGC GGCTCTCCTA CGGAGCCTTC ACCAACCAGA TCTTCGCCTC TACGAGCACA 
GATTTGCCCA CATCACCCAC CAGTGAGGCT CCCCCTTTGC CACCTCGGAA CGCCGGGAAA 
GGTCCAACTG GCCCACCTTC AACACTCCCT CTAGGCACCC AGACCTCTAG TGGCAGCTCC 
ACCCTATCCA AGAAGAGGCC TCCTCCCCCA CCACCAGGAC ACAAGAGAAC CCTGTCTGAC 
CCTCCCAGCC CACTACCTCA CGGGCCCCCA AACAAAGGCG CAATTCCTTG GGGTAATGAT 
GTGGGCCCAT TATCTTCAAG TAAGACGGCC AACAAGTTTG AGGGGCTGTC TCAGCAAGCA 
AGCACCAGTT CTGCTAAGAC TGCCCTTGGC CCGAGAGTGC TTCCTAAACT ACCTCAGAAA 
GTGGCACTAA GGAAGACGGA GACCAGCCAT CATCTCTCCC TCGACAGAAC CAACATCCCA 
CCTGAGACTT TTCAGAAATC ATCACAGTTG ACAGAGTTAC CCCAAAAGCC ACCACTTGGA 
GAGCTGCCCC CGAAGCCTGT GGAACTGGCC CCCAAGCCCC AAGTTGGAGA GCTGCCACCT 
AAGCCTGGAG AGCTACCCCC TAAGCCCCAA TTAGGTGACC TGCCCCCCAA GCCACAGCTC 
TCAGACTTAC CTCCCAAGCC ACAGATGAAG GACCTGCCCC CTAAGCCGCA GCTGGGGGAT 
CTGCTGGCAA AGTCCCAGGC TGGCGATGTC TCAGCCAAGG TGCAGCCACC CTCAGAGGTC 
ACACAGAGGT CACACACCGG GGATCTGTCT CCAAATGTAC AGTCCAGAGA TGCCATCCAG 
AAGCAAGCAT CTGAAGACTC CAACGACCTC ACACCCACGC TGCCAGAGAC ACCCGTACCA 
CTGCCGAGAA AAATCAATAC GGGGAAAAAT AAAGTGAGGC GGGTGAAGAC CATTTATGAC 
TGCCAGGCAG ATAATGACGA CGAACTCACA TTTATTGAGG GGGAAGTGAT CATTGTCACC 
GGGGAAGAGG ACCAGGAGTG GTGGATCGGG CATATCGAAG GACAGCCTGA AAGGAAGGGT 
GTCTTTCCAG TGTCCTTTGT CCACATCCTG TCTGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asap1 ( NM_001276461.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001263390.1 Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant 2, mRNA.

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CloneID	OMu17289
Clone ID Related Accession (Same CDS sequence)	NM_001276461.1
Accession Version	NM_001276461.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC094581.1, AK146545.1, AK147689.1, DV041486.1, AA266485.1 and AC156573.3. Transcript Variant: This variant (2) lacks two in-frame exons in the coding region, compared to variant 1. The encoded isoform (b) is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK146545.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGAGATCTT CAGCCTCCCG GCTCTCCAGT TTTTCCTCCA GAGATTCACT ATGGAATCGG 
ATGCCGGACC AGATCTCCGT GTCCGAGTTC ATCGCCGAGA CCACCGAGGA CTACAACTCG 
CCCACCACGT CCAGCTTCAC CACGCGGCTG CACAACTGCA GGAACACCGT CACGCTGCTG 
GAGGAGGCTC TAGACCAAGA TAGAACAGCC CTACAGAAAG TGAAGAAATC TGTAAAAGCA 
ATATATAATT CCGGTCAAGA TCATGTACAA AATGAAGAAA ACTATGCACA AGTTCTGGAT 
AAGTTTGGAA GTAATTTTTT AAGCAGAGAC AACCCTGACC TTGGCACCGC TTTTGTCAAG 
TTTTCTACAC TCACAAAGGA ACTGTCCACG CTGCTGAAAA ATCTGCTCCA GGGTCTGAGC 
CACAATGTGA TCTTCACCTT GGATTCCTTG TTAAAAGGAG ACTTGAAGGG GGTCAAAGGA 
GATCTCAAGA AGCCATTTGA CAAAGCCTGG AAAGATTATG AGACGAAGTT TACAAAAATT 
GAGAAGGAGA AAAGAGAGCA CGCAAAACAG CACGGGATGA TCCGAACAGA GATAACAGGC 
GCTGAGATTG CAGAAGAAAT GGAAAAGGAA CGGCGGCTTT TTCAGCTCCA GATGTGTGAA 
TATCTCATTA AAGTAAATGA AATCAAGACC AAAAAGGGTG TGGACCTGCT GCAGAACCTT 
ATAAAGTATT ACCATGCACA GTGCAATTTC TTTCAGGATG GCTTGAAAAC GGCTGATAAA 
CTGAAACAGT ACATAGAAAA GCTGGCTGCT GACTTATATA ATATAAAGCA AACCCAAGAT 
GAAGAAAAAA AACAGCTAAC TGCACTCCGA GATCTAATAA AATCCTCTCT GCAACTTGAT 
CCGAAAGAAG TAGGTGGTTT ATATGTTGCC AGCAGGGCTA ACAGTGACTC TCAGAGCCGG 
CAAGGTGGGT ACAGCATGCA TCAGCTCCAG GGCAACAAGG AGTACGGCAG TGAAAAGAAG 
GGCTTCCTGC TGAAGAAGAG TGATGGGATC CGGAAAGTGT GGCAGAGACG GAAGTGTGCG 
GTCAAGAACG GGATCCTGAC CATCTCCCAC GCAACTTCCA ACAGGCAGCC TGCTAAGTTA 
AACCTCCTCA CCTGCCAAGT GAAACCGAAT GCTGAGGACA AGAAGTCTTT TGACCTGATA 
TCACATAATC GAACATATCA CTTTCAAGCA GAAGATGAGC AGGATTATAT AGCGTGGATA 
TCAGTACTGA CGAATAGCAA AGAAGAGGCC CTAACCATGG CCTTCCGTGG TGAGCAGAGC 
ACAGGGGAGA ATAGCCTGGA GGACCTTACC AAAGCCATCA TCGAGGATGT ACAGCGGCTC 
CCTGGGAATG ACATCTGCTG TGACTGCGGC TCATCAGAAC CCACGTGGCT TTCAACCAAC 
TTGGGTATTT TGACCTGTAT AGAATGTTCC GGAATCCATA GGGAAATGGG GGTTCATATT 
TCTCGCATTC AGTCTTTGGA ACTAGACAAA TTAGGAACTT CCGAACTCTT GCTGGCCAAG 
AATGTAGGAA ACAATAGTTT TAATGATATT ATGGAAGCAA ATTTACCCAG CCCTTCACCA 
AAGCCCACCC CTTCAAGTGA TATGACCGTG CGTAAAGAGT ACATCACTGC AAAGTATGTC 
GACCACCGCT TCTCCAGGAA GACCTGTGCC TCTTCCTCAG CAAAGCTGAA CGAATTGCTT 
GAGGCCATCA AGTCCAGGGA CTTACTTGCA TTAATCCAAG TCTATGCAGA GGGGGTGGAG 
TTAATGGAGC CACTGCTGGA ACCTGGGCAG GAGCTCGGGG AGACAGCCCT TCATCTTGCC 
GTCAGGACCG CAGACCAGAC ATCTCTCCAT CTGGTTGATT TCCTTGTACA AAACTGTGGG 
AACCTGGATA AGCAAACGTC TGTGGGGAAT ACAGTTCTGC ACTATTGCAG CATGTACGGC 
AAGCCGGAGT GTCTGAAGCT GCTGCTTAGG AGCAAGCCCA CCGTGGACAT CGTTAACCAG 
AATGGAGAAA CTGCCTTGGA TATAGCAAAG AGACTCAAAG CTACCCAGTG TGAAGATCTG 
CTTTCCCAGG CTAAATCTGG AAAGTTCAAC CCTCATGTCC ACGTAGAGTA TGAGTGGAAT 
CTTCGACAGG ACGAGATGGA TGAGAGCGAT GACGATCTGG ATGACAAGCC AAGCCCGATC 
AAAAAGGAAC GCTCGCCCAG ACCACAGAGC TTCTGCCACT CCTCCAGCAT CTCGCCACAG 
GACAAGCTGG CACTGCCAGG GTTCAGCACT CCACGGGACA AGCAGCGGCT CTCCTACGGA 
GCCTTCACCA ACCAGATCTT CGCCTCTACG AGCACAGATT TGCCCACATC ACCCACCAGT 
GAGGCTCCCC CTTTGCCACC TCGGAACGCC GGGAAAGGTA ATGATGTGGG CCCATTATCT 
TCAAGTAAGA CGGCCAACAA GTTTGAGGGG CTGTCTCAGC AAGCAAGCAC CAGTTCTGCT 
AAGACTGCCC TTGGCCCGAG AGTGCTTCCT AAACTACCTC AGAAAGTGGC ACTAAGGAAG 
ACGGAGACCA GCCATCATCT CTCCCTCGAC AGAACCAACA TCCCACCTGA GACTTTTCAG 
AAATCATCAC AGTTGACAGA GTTACCCCAA AAGCCACCAC TTGGAGAGCT GCCCCCGAAG 
CCTGTGGAAC TGGCCCCCAA GCCCCAAGTT GGAGAGCTGC CACCTAAGCC TGGAGAGCTA 
CCCCCTAAGC CCCAATTAGG TGACCTGCCC CCCAAGCCAC AGCTCTCAGA CTTACCTCCC 
AAGCCACAGA TGAAGGACCT GCCCCCTAAG CCGCAGCTGG GGGATCTGCT GGCAAAGTCC 
CAGGCTGGCG ATGTCTCAGC CAAGGTGCAG CCACCCTCAG AGGTCACACA GAGGTCACAC 
ACCGGGGATC TGTCTCCAAA TGTACAGTCC AGAGATGCCA TCCAGAAGCA AGCATCTGAA 
GACTCCAACG ACCTCACACC CACGCTGCCA GAGACACCCG TACCACTGCC GAGAAAAATC 
AATACGGGGA AAAATAAAGT GAGGCGGGTG AAGACCATTT ATGACTGCCA GGCAGATAAT 
GACGACGAAC TCACATTTAT TGAGGGGGAA GTGATCATTG TCACCGGGGA AGAGGACCAG 
GAGTGGTGGA TCGGGCATAT CGAAGGACAG CCTGAAAGGA AGGGTGTCTT TCCAGTGTCC 
TTTGTCCACA TCCTGTCTGA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001263390.1
CDS	231..3494
Translation	MRSSASRLSSFSSRDSLWNRMPDQISVSEFIAETTEDYNSPTTSSFTTRLHNCRNTVTLLEEALDQDRTALQKVKKSVKAIYNSGQDHVQNEENYAQVLDKFGSNFLSRDNPDLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGVKGDLKKPFDKAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDPKEVGGLYVASRANSDSQSRQGGYSMHQLQGNKEYGSEKKGFLLKKSDGIRKVWQRRKCAVKNGILTISHATSNRQPAKLNLLTCQVKPNAEDKKSFDLISHNRTYHFQAEDEQDYIAWISVLTNSKEEALTMAFRGEQSTGENSLEDLTKAIIEDVQRLPGNDICCDCGSSEPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDKLGTSELLLAKNVGNNSFNDIMEANLPSPSPKPTPSSDMTVRKEYITAKYVDHRFSRKTCASSSAKLNELLEAIKSRDLLALIQVYAEGVELMEPLLEPGQELGETALHLAVRTADQTSLHLVDFLVQNCGNLDKQTSVGNTVLHYCSMYGKPECLKLLLRSKPTVDIVNQNGETALDIAKRLKATQCEDLLSQAKSGKFNPHVHVEYEWNLRQDEMDESDDDLDDKPSPIKKERSPRPQSFCHSSSISPQDKLALPGFSTPRDKQRLSYGAFTNQIFASTSTDLPTSPTSEAPPLPPRNAGKGNDVGPLSSSKTANKFEGLSQQASTSSAKTALGPRVLPKLPQKVALRKTETSHHLSLDRTNIPPETFQKSSQLTELPQKPPLGELPPKPVELAPKPQVGELPPKPGELPPKPQLGDLPPKPQLSDLPPKPQMKDLPPKPQLGDLLAKSQAGDVSAKVQPPSEVTQRSHTGDLSPNVQSRDAIQKQASEDSNDLTPTLPETPVPLPRKINTGKNKVRRVKTIYDCQADNDDELTFIEGEVIIVTGEEDQEWWIGHIEGQPERKGVFPVSFVHILSD

Target ORF information:

RefSeq Version	NM_001276461.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001276461.1

ORF Insert Sequence:

ATGAGATCTT CAGCCTCCCG GCTCTCCAGT TTTTCCTCCA GAGATTCACT ATGGAATCGG 
ATGCCGGACC AGATCTCCGT GTCCGAGTTC ATCGCCGAGA CCACCGAGGA CTACAACTCG 
CCCACCACGT CCAGCTTCAC CACGCGGCTG CACAACTGCA GGAACACCGT CACGCTGCTG 
GAGGAGGCTC TAGACCAAGA TAGAACAGCC CTACAGAAAG TGAAGAAATC TGTAAAAGCA 
ATATATAATT CCGGTCAAGA TCATGTACAA AATGAAGAAA ACTATGCACA AGTTCTGGAT 
AAGTTTGGAA GTAATTTTTT AAGCAGAGAC AACCCTGACC TTGGCACCGC TTTTGTCAAG 
TTTTCTACAC TCACAAAGGA ACTGTCCACG CTGCTGAAAA ATCTGCTCCA GGGTCTGAGC 
CACAATGTGA TCTTCACCTT GGATTCCTTG TTAAAAGGAG ACTTGAAGGG GGTCAAAGGA 
GATCTCAAGA AGCCATTTGA CAAAGCCTGG AAAGATTATG AGACGAAGTT TACAAAAATT 
GAGAAGGAGA AAAGAGAGCA CGCAAAACAG CACGGGATGA TCCGAACAGA GATAACAGGC 
GCTGAGATTG CAGAAGAAAT GGAAAAGGAA CGGCGGCTTT TTCAGCTCCA GATGTGTGAA 
TATCTCATTA AAGTAAATGA AATCAAGACC AAAAAGGGTG TGGACCTGCT GCAGAACCTT 
ATAAAGTATT ACCATGCACA GTGCAATTTC TTTCAGGATG GCTTGAAAAC GGCTGATAAA 
CTGAAACAGT ACATAGAAAA GCTGGCTGCT GACTTATATA ATATAAAGCA AACCCAAGAT 
GAAGAAAAAA AACAGCTAAC TGCACTCCGA GATCTAATAA AATCCTCTCT GCAACTTGAT 
CCGAAAGAAG TAGGTGGTTT ATATGTTGCC AGCAGGGCTA ACAGTGACTC TCAGAGCCGG 
CAAGGTGGGT ACAGCATGCA TCAGCTCCAG GGCAACAAGG AGTACGGCAG TGAAAAGAAG 
GGCTTCCTGC TGAAGAAGAG TGATGGGATC CGGAAAGTGT GGCAGAGACG GAAGTGTGCG 
GTCAAGAACG GGATCCTGAC CATCTCCCAC GCAACTTCCA ACAGGCAGCC TGCTAAGTTA 
AACCTCCTCA CCTGCCAAGT GAAACCGAAT GCTGAGGACA AGAAGTCTTT TGACCTGATA 
TCACATAATC GAACATATCA CTTTCAAGCA GAAGATGAGC AGGATTATAT AGCGTGGATA 
TCAGTACTGA CGAATAGCAA AGAAGAGGCC CTAACCATGG CCTTCCGTGG TGAGCAGAGC 
ACAGGGGAGA ATAGCCTGGA GGACCTTACC AAAGCCATCA TCGAGGATGT ACAGCGGCTC 
CCTGGGAATG ACATCTGCTG TGACTGCGGC TCATCAGAAC CCACGTGGCT TTCAACCAAC 
TTGGGTATTT TGACCTGTAT AGAATGTTCC GGAATCCATA GGGAAATGGG GGTTCATATT 
TCTCGCATTC AGTCTTTGGA ACTAGACAAA TTAGGAACTT CCGAACTCTT GCTGGCCAAG 
AATGTAGGAA ACAATAGTTT TAATGATATT ATGGAAGCAA ATTTACCCAG CCCTTCACCA 
AAGCCCACCC CTTCAAGTGA TATGACCGTG CGTAAAGAGT ACATCACTGC AAAGTATGTC 
GACCACCGCT TCTCCAGGAA GACCTGTGCC TCTTCCTCAG CAAAGCTGAA CGAATTGCTT 
GAGGCCATCA AGTCCAGGGA CTTACTTGCA TTAATCCAAG TCTATGCAGA GGGGGTGGAG 
TTAATGGAGC CACTGCTGGA ACCTGGGCAG GAGCTCGGGG AGACAGCCCT TCATCTTGCC 
GTCAGGACCG CAGACCAGAC ATCTCTCCAT CTGGTTGATT TCCTTGTACA AAACTGTGGG 
AACCTGGATA AGCAAACGTC TGTGGGGAAT ACAGTTCTGC ACTATTGCAG CATGTACGGC 
AAGCCGGAGT GTCTGAAGCT GCTGCTTAGG AGCAAGCCCA CCGTGGACAT CGTTAACCAG 
AATGGAGAAA CTGCCTTGGA TATAGCAAAG AGACTCAAAG CTACCCAGTG TGAAGATCTG 
CTTTCCCAGG CTAAATCTGG AAAGTTCAAC CCTCATGTCC ACGTAGAGTA TGAGTGGAAT 
CTTCGACAGG ACGAGATGGA TGAGAGCGAT GACGATCTGG ATGACAAGCC AAGCCCGATC 
AAAAAGGAAC GCTCGCCCAG ACCACAGAGC TTCTGCCACT CCTCCAGCAT CTCGCCACAG 
GACAAGCTGG CACTGCCAGG GTTCAGCACT CCACGGGACA AGCAGCGGCT CTCCTACGGA 
GCCTTCACCA ACCAGATCTT CGCCTCTACG AGCACAGATT TGCCCACATC ACCCACCAGT 
GAGGCTCCCC CTTTGCCACC TCGGAACGCC GGGAAAGGTA ATGATGTGGG CCCATTATCT 
TCAAGTAAGA CGGCCAACAA GTTTGAGGGG CTGTCTCAGC AAGCAAGCAC CAGTTCTGCT 
AAGACTGCCC TTGGCCCGAG AGTGCTTCCT AAACTACCTC AGAAAGTGGC ACTAAGGAAG 
ACGGAGACCA GCCATCATCT CTCCCTCGAC AGAACCAACA TCCCACCTGA GACTTTTCAG 
AAATCATCAC AGTTGACAGA GTTACCCCAA AAGCCACCAC TTGGAGAGCT GCCCCCGAAG 
CCTGTGGAAC TGGCCCCCAA GCCCCAAGTT GGAGAGCTGC CACCTAAGCC TGGAGAGCTA 
CCCCCTAAGC CCCAATTAGG TGACCTGCCC CCCAAGCCAC AGCTCTCAGA CTTACCTCCC 
AAGCCACAGA TGAAGGACCT GCCCCCTAAG CCGCAGCTGG GGGATCTGCT GGCAAAGTCC 
CAGGCTGGCG ATGTCTCAGC CAAGGTGCAG CCACCCTCAG AGGTCACACA GAGGTCACAC 
ACCGGGGATC TGTCTCCAAA TGTACAGTCC AGAGATGCCA TCCAGAAGCA AGCATCTGAA 
GACTCCAACG ACCTCACACC CACGCTGCCA GAGACACCCG TACCACTGCC GAGAAAAATC 
AATACGGGGA AAAATAAAGT GAGGCGGGTG AAGACCATTT ATGACTGCCA GGCAGATAAT 
GACGACGAAC TCACATTTAT TGAGGGGGAA GTGATCATTG TCACCGGGGA AGAGGACCAG 
GAGTGGTGGA TCGGGCATAT CGAAGGACAG CCTGAAAGGA AGGGTGTCTT TCCAGTGTCC 
TTTGTCCACA TCCTGTCTGA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asap1 ( XM_006520392.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006520455.1 Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X1, mRNA.

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CloneID	OMu50913
Clone ID Related Accession (Same CDS sequence)	XM_006520392.2
Accession Version	XM_006520392.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3672bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006520392.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAGAT GCATGCTTTG CATTCCTTTT GAATTCCCTT TGAGTCCCCA GGTCAGGCTC 
CAACACCATG TAAGGGATGG GCTTTCGACT GTGCTCATCC TGCCTTTCAT GTCTGCTCTG 
CTTCCCCAGC GTGAAGCTAG TGCCGAAGAG CTCCGCCAGC TTTATGAGTG GAGCAGGAAC 
CCACACTCAT TAAACACAAC AGGATGCCGG ACCAGATCTC CGTGTCCGAG TTCATCGCCG 
AGACCACCGA GGACTACAAC TCGCCCACCA CGTCCAGCTT CACCACGCGG CTGCACAACT 
GCAGGAACAC CGTCACGCTG CTGGAGGAGC GTGGTTGACG GTGGCAGCCG AGCAGTGGTG 
CAGATGACGC CAGCTGTGTT GCACTTGGGT GAGCCATACA CAAGTTCATT TGAGGCTCTA 
GACCAAGATA GAACAGCCCT ACAGAAAGTG AAGAAATCTG TAAAAGCAAT ATATAATTCC 
GGTCAAGATC ATGTACAAAA TGAAGAAAAC TATGCACAAG TTCTGGATAA GTTTGGAAGT 
AATTTTTTAA GCAGAGACAA CCCTGACCTT GGCACCGCTT TTGTCAAGTT TTCTACACTC 
ACAAAGGAAC TGTCCACGCT GCTGAAAAAT CTGCTCCAGG GTCTGAGCCA CAATGTGATC 
TTCACCTTGG ATTCCTTGTT AAAAGGAGAC TTGAAGGGGG TCAAAGGAGA TCTCAAGAAG 
CCATTTGACA AAGCCTGGAA AGATTATGAG ACGAAGTTTA CAAAAATTGA GAAGGAGAAA 
AGAGAGCACG CAAAACAGCA CGGGATGATC CGAACAGAGA TAACAGGCGC TGAGATTGCA 
GAAGAAATGG AAAAGGAACG GCGGCTTTTT CAGCTCCAGA TGTGTGAATA TCTCATTAAA 
GTAAATGAAA TCAAGACCAA AAAGGGTGTG GACCTGCTGC AGAACCTTAT AAAGTATTAC 
CATGCACAGT GCAATTTCTT TCAGGATGGC TTGAAAACGG CTGATAAACT GAAACAGTAC 
ATAGAAAAGC TGGCTGCTGA CTTATATAAT ATAAAGCAAA CCCAAGATGA AGAAAAAAAA 
CAGCTAACTG CACTCCGAGA TCTAATAAAA TCCTCTCTGC AACTTGATCC GAAAGAAGTA 
GGTGGTTTAT ATGTTGCCAG CAGGGCTAAC AGTTCTAGAA GAGACTCTCA GAGCCGGCAA 
GGTGGGTACA GCATGCATCA GCTCCAGGGC AACAAGGAGT ACGGCAGTGA AAAGAAGGGC 
TTCCTGCTGA AGAAGAGTGA TGGGATCCGG AAAGTGTGGC AGAGACGGAA GTGTGCGGTC 
AAGAACGGGA TCCTGACCAT CTCCCACGCA ACTTCCAACA GGCAGCCTGC TAAGTTAAAC 
CTCCTCACCT GCCAAGTGAA ACCGAATGCT GAGGACAAGA AGTCTTTTGA CCTGATATCA 
CATAATCGAA CATATCACTT TCAAGCAGAA GATGAGCAGG ATTATATAGC GTGGATATCA 
GTACTGACGA ATAGCAAAGA AGAGGCCCTA ACCATGGCCT TCCGTGGTGA GCAGAGCACA 
GGGGAGAATA GCCTGGAGGA CCTTACCAAA GCCATCATCG AGGATGTACA GCGGCTCCCT 
GGGAATGACA TCTGCTGTGA CTGCGGCTCA TCAGAACCCA CGTGGCTTTC AACCAACTTG 
GGTATTTTGA CCTGTATAGA ATGTTCCGGA ATCCATAGGG AAATGGGGGT TCATATTTCT 
CGCATTCAGT CTTTGGAACT AGACAAATTA GGAACTTCCG AACTCTTGCT GGCCAAGAAT 
GTAGGAAACA ATAGTTTTAA TGATATTATG GAAGCAAATT TACCCAGCCC TTCACCAAAG 
CCCACCCCTT CAAGTGATAT GACCGTGCGT AAAGAGTACA TCACTGCAAA GTATGTCGAC 
CACCGCTTCT CCAGGAAGAC CTGTGCCTCT TCCTCAGCAA AGCTGAACGA ATTGCTTGAG 
GCCATCAAGT CCAGGGACTT ACTTGCATTA ATCCAAGTCT ATGCAGAGGG GGTGGAGTTA 
ATGGAGCCAC TGCTGGAACC TGGGCAGGAG CTCGGGGAGA CAGCCCTTCA TCTTGCCGTC 
AGGACCGCAG ACCAGACATC TCTCCATCTG GTTGATTTCC TTGTACAAAA CTGTGGGAAC 
CTGGATAAGC AAACGTCTGT GGGGAATACA GTTCTGCACT ATTGCAGCAT GTACGGCAAG 
CCGGAGTGTC TGAAGCTGCT GCTTAGGAGC AAGCCCACCG TGGACATCGT TAACCAGAAT 
GGAGAAACTG CCTTGGATAT AGCAAAGAGA CTCAAAGCTA CCCAGTGTGA AGATCTGCTT 
TCCCAGGCTA AATCTGGAAA GTTCAACCCT CATGTCCACG TAGAGTATGA GTGGAATCTT 
CGACAGGACG AGATGGATGA GAGCGATGAC GATCTGGATG ACAAGCCAAG CCCGATCAAA 
AAGGAACGCT CGCCCAGACC ACAGAGCTTC TGCCACTCCT CCAGCATCTC GCCACAGGAC 
AAGCTGGCAC TGCCAGGGTT CAGCACTCCA CGGGACAAGC AGCGGCTCTC CTACGGAGCC 
TTCACCAACC AGATCTTCGC CTCTACGAGC ACAGATTTGC CCACATCACC CACCAGTGAG 
GCTCCCCCTT TGCCACCTCG GAACGCCGGG AAAGGTCCAA CTGGCCCACC TTCAACACTC 
CCTCTAGGCA CCCAGACCTC TAGTGGCAGC TCCACCCTAT CCAAGAAGAG GCCTCCTCCC 
CCACCACCAG GACACAAGAG AACCCTGTCT GACCCTCCCA GCCCACTACC TCACGGGCCC 
CCAAACAAAG GCGCAATTCC TTGGGGTAAT GATGTGGGCC CATTATCTTC AAGTAAGACG 
GCCAACAAGT TTGAGGGGCT GTCTCAGCAA GCAAGCACCA GTTCTGCTAA GACTGCCCTT 
GGCCCGAGAG TGCTTCCTAA ACTACCTCAG AAAGTGGCAC TAAGGAAGAC GGAGACCAGC 
CATCATCTCT CCCTCGACAG AACCAACATC CCACCTGAGA CTTTTCAGAA ATCATCACAG 
TTGACAGAGT TACCCCAAAA GCCACCACTT GGAGAGCTGC CCCCGAAGCC TGTGGAACTG 
GCCCCCAAGC CCCAAGTTGG AGAGCTGCCA CCTAAGCCTG GAGAGCTACC CCCTAAGCCC 
CAATTAGGTG ACCTGCCCCC CAAGCCACAG CTCTCAGACT TACCTCCCAA GCCACAGATG 
AAGGACCTGC CCCCTAAGCC GCAGCTGGGG GATCTGCTGG CAAAGTCCCA GGCTGGCGAT 
GTCTCAGCCA AGGTGCAGCC ACCCTCAGAG GTCACACAGA GGTCACACAC CGGGGATCTG 
TCTCCAAATG TACAGTCCAG AGATGCCATC CAGAAGCAAG CATCTGAAGA CTCCAACGAC 
CTCACACCCA CGCTGCCAGA GACACCCGTA CCACTGCCGA GAAAAATCAA TACGGGGAAA 
AATAAAGTGA GGCGGGTGAA GACCATTTAT GACTGCCAGG CAGATAATGA CGACGAACTC 
ACATTTATTG AGGGGGAAGT GATCATTGTC ACCGGGGAAG AGGACCAGGA GTGGTGGATC 
GGGCATATCG AAGGACAGCC TGAAAGGAAG GGTGTCTTTC CAGTGTCCTT TGTCCACATC 
CTGTCTGACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006520455.1
CDS	16..3687
Translation	MSRCMLCIPFEFPLSPQVRLQHHVRDGLSTVLILPFMSALLPQREASAEELRQLYEWSRNPHSLNTTGCRTRSPCPSSSPRPPRTTTRPPRPASPRGCTTAGTPSRCWRSVVDGGSRAVVQMTPAVLHLGEPYTSSFEALDQDRTALQKVKKSVKAIYNSGQDHVQNEENYAQVLDKFGSNFLSRDNPDLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGVKGDLKKPFDKAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDPKEVGGLYVASRANSSRRDSQSRQGGYSMHQLQGNKEYGSEKKGFLLKKSDGIRKVWQRRKCAVKNGILTISHATSNRQPAKLNLLTCQVKPNAEDKKSFDLISHNRTYHFQAEDEQDYIAWISVLTNSKEEALTMAFRGEQSTGENSLEDLTKAIIEDVQRLPGNDICCDCGSSEPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDKLGTSELLLAKNVGNNSFNDIMEANLPSPSPKPTPSSDMTVRKEYITAKYVDHRFSRKTCASSSAKLNELLEAIKSRDLLALIQVYAEGVELMEPLLEPGQELGETALHLAVRTADQTSLHLVDFLVQNCGNLDKQTSVGNTVLHYCSMYGKPECLKLLLRSKPTVDIVNQNGETALDIAKRLKATQCEDLLSQAKSGKFNPHVHVEYEWNLRQDEMDESDDDLDDKPSPIKKERSPRPQSFCHSSSISPQDKLALPGFSTPRDKQRLSYGAFTNQIFASTSTDLPTSPTSEAPPLPPRNAGKGPTGPPSTLPLGTQTSSGSSTLSKKRPPPPPPGHKRTLSDPPSPLPHGPPNKGAIPWGNDVGPLSSSKTANKFEGLSQQASTSSAKTALGPRVLPKLPQKVALRKTETSHHLSLDRTNIPPETFQKSSQLTELPQKPPLGELPPKPVELAPKPQVGELPPKPGELPPKPQLGDLPPKPQLSDLPPKPQMKDLPPKPQLGDLLAKSQAGDVSAKVQPPSEVTQRSHTGDLSPNVQSRDAIQKQASEDSNDLTPTLPETPVPLPRKINTGKNKVRRVKTIYDCQADNDDELTFIEGEVIIVTGEEDQEWWIGHIEGQPERKGVFPVSFVHILSD

Target ORF information:

RefSeq Version	XM_006520392.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006520392.2

ORF Insert Sequence:

ATGTCGAGAT GCATGCTTTG CATTCCTTTT GAATTCCCTT TGAGTCCCCA GGTCAGGCTC 
CAACACCATG TAAGGGATGG GCTTTCGACT GTGCTCATCC TGCCTTTCAT GTCTGCTCTG 
CTTCCCCAGC GTGAAGCTAG TGCCGAAGAG CTCCGCCAGC TTTATGAGTG GAGCAGGAAC 
CCACACTCAT TAAACACAAC AGGATGCCGG ACCAGATCTC CGTGTCCGAG TTCATCGCCG 
AGACCACCGA GGACTACAAC TCGCCCACCA CGTCCAGCTT CACCACGCGG CTGCACAACT 
GCAGGAACAC CGTCACGCTG CTGGAGGAGC GTGGTTGACG GTGGCAGCCG AGCAGTGGTG 
CAGATGACGC CAGCTGTGTT GCACTTGGGT GAGCCATACA CAAGTTCATT TGAGGCTCTA 
GACCAAGATA GAACAGCCCT ACAGAAAGTG AAGAAATCTG TAAAAGCAAT ATATAATTCC 
GGTCAAGATC ATGTACAAAA TGAAGAAAAC TATGCACAAG TTCTGGATAA GTTTGGAAGT 
AATTTTTTAA GCAGAGACAA CCCTGACCTT GGCACCGCTT TTGTCAAGTT TTCTACACTC 
ACAAAGGAAC TGTCCACGCT GCTGAAAAAT CTGCTCCAGG GTCTGAGCCA CAATGTGATC 
TTCACCTTGG ATTCCTTGTT AAAAGGAGAC TTGAAGGGGG TCAAAGGAGA TCTCAAGAAG 
CCATTTGACA AAGCCTGGAA AGATTATGAG ACGAAGTTTA CAAAAATTGA GAAGGAGAAA 
AGAGAGCACG CAAAACAGCA CGGGATGATC CGAACAGAGA TAACAGGCGC TGAGATTGCA 
GAAGAAATGG AAAAGGAACG GCGGCTTTTT CAGCTCCAGA TGTGTGAATA TCTCATTAAA 
GTAAATGAAA TCAAGACCAA AAAGGGTGTG GACCTGCTGC AGAACCTTAT AAAGTATTAC 
CATGCACAGT GCAATTTCTT TCAGGATGGC TTGAAAACGG CTGATAAACT GAAACAGTAC 
ATAGAAAAGC TGGCTGCTGA CTTATATAAT ATAAAGCAAA CCCAAGATGA AGAAAAAAAA 
CAGCTAACTG CACTCCGAGA TCTAATAAAA TCCTCTCTGC AACTTGATCC GAAAGAAGTA 
GGTGGTTTAT ATGTTGCCAG CAGGGCTAAC AGTTCTAGAA GAGACTCTCA GAGCCGGCAA 
GGTGGGTACA GCATGCATCA GCTCCAGGGC AACAAGGAGT ACGGCAGTGA AAAGAAGGGC 
TTCCTGCTGA AGAAGAGTGA TGGGATCCGG AAAGTGTGGC AGAGACGGAA GTGTGCGGTC 
AAGAACGGGA TCCTGACCAT CTCCCACGCA ACTTCCAACA GGCAGCCTGC TAAGTTAAAC 
CTCCTCACCT GCCAAGTGAA ACCGAATGCT GAGGACAAGA AGTCTTTTGA CCTGATATCA 
CATAATCGAA CATATCACTT TCAAGCAGAA GATGAGCAGG ATTATATAGC GTGGATATCA 
GTACTGACGA ATAGCAAAGA AGAGGCCCTA ACCATGGCCT TCCGTGGTGA GCAGAGCACA 
GGGGAGAATA GCCTGGAGGA CCTTACCAAA GCCATCATCG AGGATGTACA GCGGCTCCCT 
GGGAATGACA TCTGCTGTGA CTGCGGCTCA TCAGAACCCA CGTGGCTTTC AACCAACTTG 
GGTATTTTGA CCTGTATAGA ATGTTCCGGA ATCCATAGGG AAATGGGGGT TCATATTTCT 
CGCATTCAGT CTTTGGAACT AGACAAATTA GGAACTTCCG AACTCTTGCT GGCCAAGAAT 
GTAGGAAACA ATAGTTTTAA TGATATTATG GAAGCAAATT TACCCAGCCC TTCACCAAAG 
CCCACCCCTT CAAGTGATAT GACCGTGCGT AAAGAGTACA TCACTGCAAA GTATGTCGAC 
CACCGCTTCT CCAGGAAGAC CTGTGCCTCT TCCTCAGCAA AGCTGAACGA ATTGCTTGAG 
GCCATCAAGT CCAGGGACTT ACTTGCATTA ATCCAAGTCT ATGCAGAGGG GGTGGAGTTA 
ATGGAGCCAC TGCTGGAACC TGGGCAGGAG CTCGGGGAGA CAGCCCTTCA TCTTGCCGTC 
AGGACCGCAG ACCAGACATC TCTCCATCTG GTTGATTTCC TTGTACAAAA CTGTGGGAAC 
CTGGATAAGC AAACGTCTGT GGGGAATACA GTTCTGCACT ATTGCAGCAT GTACGGCAAG 
CCGGAGTGTC TGAAGCTGCT GCTTAGGAGC AAGCCCACCG TGGACATCGT TAACCAGAAT 
GGAGAAACTG CCTTGGATAT AGCAAAGAGA CTCAAAGCTA CCCAGTGTGA AGATCTGCTT 
TCCCAGGCTA AATCTGGAAA GTTCAACCCT CATGTCCACG TAGAGTATGA GTGGAATCTT 
CGACAGGACG AGATGGATGA GAGCGATGAC GATCTGGATG ACAAGCCAAG CCCGATCAAA 
AAGGAACGCT CGCCCAGACC ACAGAGCTTC TGCCACTCCT CCAGCATCTC GCCACAGGAC 
AAGCTGGCAC TGCCAGGGTT CAGCACTCCA CGGGACAAGC AGCGGCTCTC CTACGGAGCC 
TTCACCAACC AGATCTTCGC CTCTACGAGC ACAGATTTGC CCACATCACC CACCAGTGAG 
GCTCCCCCTT TGCCACCTCG GAACGCCGGG AAAGGTCCAA CTGGCCCACC TTCAACACTC 
CCTCTAGGCA CCCAGACCTC TAGTGGCAGC TCCACCCTAT CCAAGAAGAG GCCTCCTCCC 
CCACCACCAG GACACAAGAG AACCCTGTCT GACCCTCCCA GCCCACTACC TCACGGGCCC 
CCAAACAAAG GCGCAATTCC TTGGGGTAAT GATGTGGGCC CATTATCTTC AAGTAAGACG 
GCCAACAAGT TTGAGGGGCT GTCTCAGCAA GCAAGCACCA GTTCTGCTAA GACTGCCCTT 
GGCCCGAGAG TGCTTCCTAA ACTACCTCAG AAAGTGGCAC TAAGGAAGAC GGAGACCAGC 
CATCATCTCT CCCTCGACAG AACCAACATC CCACCTGAGA CTTTTCAGAA ATCATCACAG 
TTGACAGAGT TACCCCAAAA GCCACCACTT GGAGAGCTGC CCCCGAAGCC TGTGGAACTG 
GCCCCCAAGC CCCAAGTTGG AGAGCTGCCA CCTAAGCCTG GAGAGCTACC CCCTAAGCCC 
CAATTAGGTG ACCTGCCCCC CAAGCCACAG CTCTCAGACT TACCTCCCAA GCCACAGATG 
AAGGACCTGC CCCCTAAGCC GCAGCTGGGG GATCTGCTGG CAAAGTCCCA GGCTGGCGAT 
GTCTCAGCCA AGGTGCAGCC ACCCTCAGAG GTCACACAGA GGTCACACAC CGGGGATCTG 
TCTCCAAATG TACAGTCCAG AGATGCCATC CAGAAGCAAG CATCTGAAGA CTCCAACGAC 
CTCACACCCA CGCTGCCAGA GACACCCGTA CCACTGCCGA GAAAAATCAA TACGGGGAAA 
AATAAAGTGA GGCGGGTGAA GACCATTTAT GACTGCCAGG CAGATAATGA CGACGAACTC 
ACATTTATTG AGGGGGAAGT GATCATTGTC ACCGGGGAAG AGGACCAGGA GTGGTGGATC 
GGGCATATCG AAGGACAGCC TGAAAGGAAG GGTGTCTTTC CAGTGTCCTT TGTCCACATC 
CTGTCTGACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asap1 ( XM_006520393.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006520456.1 Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu50914
Clone ID Related Accession (Same CDS sequence)	XM_006520393.2
Accession Version	XM_006520393.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3663bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006520393.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAGAT GCATGCTTTG CATTCCTTTT GAATTCCCTT TGAGTCCCCA GGTCAGGCTC 
CAACACCATG TAAGGGATGG GCTTTCGACT GTGCTCATCC TGCCTTTCAT GTCTGCTCTG 
CTTCCCCAGC GTGAAGCTAG TGCCGAAGAG CTCCGCCAGC TTTATGAGTG GAGCAGGAAC 
CCACACTCAT TAAACACAAC AGGATGCCGG ACCAGATCTC CGTGTCCGAG TTCATCGCCG 
AGACCACCGA GGACTACAAC TCGCCCACCA CGTCCAGCTT CACCACGCGG CTGCACAACT 
GCAGGAACAC CGTCACGCTG CTGGAGGAGC GTGGTTGACG GTGGCAGCCG AGCAGTGGTG 
CAGATGACGC CAGCTGTGTT GCACTTGGGT GAGCCATACA CAAGTTCATT TGAGGCTCTA 
GACCAAGATA GAACAGCCCT ACAGAAAGTG AAGAAATCTG TAAAAGCAAT ATATAATTCC 
GGTCAAGATC ATGTACAAAA TGAAGAAAAC TATGCACAAG TTCTGGATAA GTTTGGAAGT 
AATTTTTTAA GCAGAGACAA CCCTGACCTT GGCACCGCTT TTGTCAAGTT TTCTACACTC 
ACAAAGGAAC TGTCCACGCT GCTGAAAAAT CTGCTCCAGG GTCTGAGCCA CAATGTGATC 
TTCACCTTGG ATTCCTTGTT AAAAGGAGAC TTGAAGGGGG TCAAAGGAGA TCTCAAGAAG 
CCATTTGACA AAGCCTGGAA AGATTATGAG ACGAAGTTTA CAAAAATTGA GAAGGAGAAA 
AGAGAGCACG CAAAACAGCA CGGGATGATC CGAACAGAGA TAACAGGCGC TGAGATTGCA 
GAAGAAATGG AAAAGGAACG GCGGCTTTTT CAGCTCCAGA TGTGTGAATA TCTCATTAAA 
GTAAATGAAA TCAAGACCAA AAAGGGTGTG GACCTGCTGC AGAACCTTAT AAAGTATTAC 
CATGCACAGT GCAATTTCTT TCAGGATGGC TTGAAAACGG CTGATAAACT GAAACAGTAC 
ATAGAAAAGC TGGCTGCTGA CTTATATAAT ATAAAGCAAA CCCAAGATGA AGAAAAAAAA 
CAGCTAACTG CACTCCGAGA TCTAATAAAA TCCTCTCTGC AACTTGATCC GAAAGAAGTA 
GGTGGTTTAT ATGTTGCCAG CAGGGCTAAC AGTGACTCTC AGAGCCGGCA AGGTGGGTAC 
AGCATGCATC AGCTCCAGGG CAACAAGGAG TACGGCAGTG AAAAGAAGGG CTTCCTGCTG 
AAGAAGAGTG ATGGGATCCG GAAAGTGTGG CAGAGACGGA AGTGTGCGGT CAAGAACGGG 
ATCCTGACCA TCTCCCACGC AACTTCCAAC AGGCAGCCTG CTAAGTTAAA CCTCCTCACC 
TGCCAAGTGA AACCGAATGC TGAGGACAAG AAGTCTTTTG ACCTGATATC ACATAATCGA 
ACATATCACT TTCAAGCAGA AGATGAGCAG GATTATATAG CGTGGATATC AGTACTGACG 
AATAGCAAAG AAGAGGCCCT AACCATGGCC TTCCGTGGTG AGCAGAGCAC AGGGGAGAAT 
AGCCTGGAGG ACCTTACCAA AGCCATCATC GAGGATGTAC AGCGGCTCCC TGGGAATGAC 
ATCTGCTGTG ACTGCGGCTC ATCAGAACCC ACGTGGCTTT CAACCAACTT GGGTATTTTG 
ACCTGTATAG AATGTTCCGG AATCCATAGG GAAATGGGGG TTCATATTTC TCGCATTCAG 
TCTTTGGAAC TAGACAAATT AGGAACTTCC GAACTCTTGC TGGCCAAGAA TGTAGGAAAC 
AATAGTTTTA ATGATATTAT GGAAGCAAAT TTACCCAGCC CTTCACCAAA GCCCACCCCT 
TCAAGTGATA TGACCGTGCG TAAAGAGTAC ATCACTGCAA AGTATGTCGA CCACCGCTTC 
TCCAGGAAGA CCTGTGCCTC TTCCTCAGCA AAGCTGAACG AATTGCTTGA GGCCATCAAG 
TCCAGGGACT TACTTGCATT AATCCAAGTC TATGCAGAGG GGGTGGAGTT AATGGAGCCA 
CTGCTGGAAC CTGGGCAGGA GCTCGGGGAG ACAGCCCTTC ATCTTGCCGT CAGGACCGCA 
GACCAGACAT CTCTCCATCT GGTTGATTTC CTTGTACAAA ACTGTGGGAA CCTGGATAAG 
CAAACGTCTG TGGGGAATAC AGTTCTGCAC TATTGCAGCA TGTACGGCAA GCCGGAGTGT 
CTGAAGCTGC TGCTTAGGAG CAAGCCCACC GTGGACATCG TTAACCAGAA TGGAGAAACT 
GCCTTGGATA TAGCAAAGAG ACTCAAAGCT ACCCAGTGTG AAGATCTGCT TTCCCAGGCT 
AAATCTGGAA AGTTCAACCC TCATGTCCAC GTAGAGTATG AGTGGAATCT TCGACAGGAC 
GAGATGGATG AGAGCGATGA CGATCTGGAT GACAAGCCAA GCCCGATCAA AAAGGAACGC 
TCGCCCAGAC CACAGAGCTT CTGCCACTCC TCCAGCATCT CGCCACAGGA CAAGCTGGCA 
CTGCCAGGGT TCAGCACTCC ACGGGACAAG CAGCGGCTCT CCTACGGAGC CTTCACCAAC 
CAGATCTTCG CCTCTACGAG CACAGATTTG CCCACATCAC CCACCAGTGA GGCTCCCCCT 
TTGCCACCTC GGAACGCCGG GAAAGGTCCA ACTGGCCCAC CTTCAACACT CCCTCTAGGC 
ACCCAGACCT CTAGTGGCAG CTCCACCCTA TCCAAGAAGA GGCCTCCTCC CCCACCACCA 
GGACACAAGA GAACCCTGTC TGACCCTCCC AGCCCACTAC CTCACGGGCC CCCAAACAAA 
GGCGCAATTC CTTGGGGTAA TGATGTGGGC CCATTATCTT CAAGTAAGAC GGCCAACAAG 
TTTGAGGGGC TGTCTCAGCA AGCAAGCACC AGTTCTGCTA AGACTGCCCT TGGCCCGAGA 
GTGCTTCCTA AACTACCTCA GAAAGTGGCA CTAAGGAAGA CGGAGACCAG CCATCATCTC 
TCCCTCGACA GAACCAACAT CCCACCTGAG ACTTTTCAGA AATCATCACA GTTGACAGAG 
TTACCCCAAA AGCCACCACT TGGAGAGCTG CCCCCGAAGC CTGTGGAACT GGCCCCCAAG 
CCCCAAGTTG GAGAGCTGCC ACCTAAGCCT GGAGAGCTAC CCCCTAAGCC CCAATTAGGT 
GACCTGCCCC CCAAGCCACA GCTCTCAGAC TTACCTCCCA AGCCACAGAT GAAGGACCTG 
CCCCCTAAGC CGCAGCTGGG GGATCTGCTG GCAAAGTCCC AGGCTGGCGA TGTCTCAGCC 
AAGGTGCAGC CACCCTCAGA GGTCACACAG AGGTCACACA CCGGGGATCT GTCTCCAAAT 
GTACAGTCCA GAGATGCCAT CCAGAAGCAA GCATCTGAAG ACTCCAACGA CCTCACACCC 
ACGCTGCCAG AGACACCCGT ACCACTGCCG AGAAAAATCA ATACGGGGAA AAATAAAGTG 
AGGCGGGTGA AGACCATTTA TGACTGCCAG GCAGATAATG ACGACGAACT CACATTTATT 
GAGGGGGAAG TGATCATTGT CACCGGGGAA GAGGACCAGG AGTGGTGGAT CGGGCATATC 
GAAGGACAGC CTGAAAGGAA GGGTGTCTTT CCAGTGTCCT TTGTCCACAT CCTGTCTGAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006520456.1
CDS	16..3678
Translation	MSRCMLCIPFEFPLSPQVRLQHHVRDGLSTVLILPFMSALLPQREASAEELRQLYEWSRNPHSLNTTGCRTRSPCPSSSPRPPRTTTRPPRPASPRGCTTAGTPSRCWRSVVDGGSRAVVQMTPAVLHLGEPYTSSFEALDQDRTALQKVKKSVKAIYNSGQDHVQNEENYAQVLDKFGSNFLSRDNPDLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGVKGDLKKPFDKAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDPKEVGGLYVASRANSDSQSRQGGYSMHQLQGNKEYGSEKKGFLLKKSDGIRKVWQRRKCAVKNGILTISHATSNRQPAKLNLLTCQVKPNAEDKKSFDLISHNRTYHFQAEDEQDYIAWISVLTNSKEEALTMAFRGEQSTGENSLEDLTKAIIEDVQRLPGNDICCDCGSSEPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDKLGTSELLLAKNVGNNSFNDIMEANLPSPSPKPTPSSDMTVRKEYITAKYVDHRFSRKTCASSSAKLNELLEAIKSRDLLALIQVYAEGVELMEPLLEPGQELGETALHLAVRTADQTSLHLVDFLVQNCGNLDKQTSVGNTVLHYCSMYGKPECLKLLLRSKPTVDIVNQNGETALDIAKRLKATQCEDLLSQAKSGKFNPHVHVEYEWNLRQDEMDESDDDLDDKPSPIKKERSPRPQSFCHSSSISPQDKLALPGFSTPRDKQRLSYGAFTNQIFASTSTDLPTSPTSEAPPLPPRNAGKGPTGPPSTLPLGTQTSSGSSTLSKKRPPPPPPGHKRTLSDPPSPLPHGPPNKGAIPWGNDVGPLSSSKTANKFEGLSQQASTSSAKTALGPRVLPKLPQKVALRKTETSHHLSLDRTNIPPETFQKSSQLTELPQKPPLGELPPKPVELAPKPQVGELPPKPGELPPKPQLGDLPPKPQLSDLPPKPQMKDLPPKPQLGDLLAKSQAGDVSAKVQPPSEVTQRSHTGDLSPNVQSRDAIQKQASEDSNDLTPTLPETPVPLPRKINTGKNKVRRVKTIYDCQADNDDELTFIEGEVIIVTGEEDQEWWIGHIEGQPERKGVFPVSFVHILSD

Target ORF information:

RefSeq Version	XM_006520393.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006520393.2

ORF Insert Sequence:

ATGTCGAGAT GCATGCTTTG CATTCCTTTT GAATTCCCTT TGAGTCCCCA GGTCAGGCTC 
CAACACCATG TAAGGGATGG GCTTTCGACT GTGCTCATCC TGCCTTTCAT GTCTGCTCTG 
CTTCCCCAGC GTGAAGCTAG TGCCGAAGAG CTCCGCCAGC TTTATGAGTG GAGCAGGAAC 
CCACACTCAT TAAACACAAC AGGATGCCGG ACCAGATCTC CGTGTCCGAG TTCATCGCCG 
AGACCACCGA GGACTACAAC TCGCCCACCA CGTCCAGCTT CACCACGCGG CTGCACAACT 
GCAGGAACAC CGTCACGCTG CTGGAGGAGC GTGGTTGACG GTGGCAGCCG AGCAGTGGTG 
CAGATGACGC CAGCTGTGTT GCACTTGGGT GAGCCATACA CAAGTTCATT TGAGGCTCTA 
GACCAAGATA GAACAGCCCT ACAGAAAGTG AAGAAATCTG TAAAAGCAAT ATATAATTCC 
GGTCAAGATC ATGTACAAAA TGAAGAAAAC TATGCACAAG TTCTGGATAA GTTTGGAAGT 
AATTTTTTAA GCAGAGACAA CCCTGACCTT GGCACCGCTT TTGTCAAGTT TTCTACACTC 
ACAAAGGAAC TGTCCACGCT GCTGAAAAAT CTGCTCCAGG GTCTGAGCCA CAATGTGATC 
TTCACCTTGG ATTCCTTGTT AAAAGGAGAC TTGAAGGGGG TCAAAGGAGA TCTCAAGAAG 
CCATTTGACA AAGCCTGGAA AGATTATGAG ACGAAGTTTA CAAAAATTGA GAAGGAGAAA 
AGAGAGCACG CAAAACAGCA CGGGATGATC CGAACAGAGA TAACAGGCGC TGAGATTGCA 
GAAGAAATGG AAAAGGAACG GCGGCTTTTT CAGCTCCAGA TGTGTGAATA TCTCATTAAA 
GTAAATGAAA TCAAGACCAA AAAGGGTGTG GACCTGCTGC AGAACCTTAT AAAGTATTAC 
CATGCACAGT GCAATTTCTT TCAGGATGGC TTGAAAACGG CTGATAAACT GAAACAGTAC 
ATAGAAAAGC TGGCTGCTGA CTTATATAAT ATAAAGCAAA CCCAAGATGA AGAAAAAAAA 
CAGCTAACTG CACTCCGAGA TCTAATAAAA TCCTCTCTGC AACTTGATCC GAAAGAAGTA 
GGTGGTTTAT ATGTTGCCAG CAGGGCTAAC AGTGACTCTC AGAGCCGGCA AGGTGGGTAC 
AGCATGCATC AGCTCCAGGG CAACAAGGAG TACGGCAGTG AAAAGAAGGG CTTCCTGCTG 
AAGAAGAGTG ATGGGATCCG GAAAGTGTGG CAGAGACGGA AGTGTGCGGT CAAGAACGGG 
ATCCTGACCA TCTCCCACGC AACTTCCAAC AGGCAGCCTG CTAAGTTAAA CCTCCTCACC 
TGCCAAGTGA AACCGAATGC TGAGGACAAG AAGTCTTTTG ACCTGATATC ACATAATCGA 
ACATATCACT TTCAAGCAGA AGATGAGCAG GATTATATAG CGTGGATATC AGTACTGACG 
AATAGCAAAG AAGAGGCCCT AACCATGGCC TTCCGTGGTG AGCAGAGCAC AGGGGAGAAT 
AGCCTGGAGG ACCTTACCAA AGCCATCATC GAGGATGTAC AGCGGCTCCC TGGGAATGAC 
ATCTGCTGTG ACTGCGGCTC ATCAGAACCC ACGTGGCTTT CAACCAACTT GGGTATTTTG 
ACCTGTATAG AATGTTCCGG AATCCATAGG GAAATGGGGG TTCATATTTC TCGCATTCAG 
TCTTTGGAAC TAGACAAATT AGGAACTTCC GAACTCTTGC TGGCCAAGAA TGTAGGAAAC 
AATAGTTTTA ATGATATTAT GGAAGCAAAT TTACCCAGCC CTTCACCAAA GCCCACCCCT 
TCAAGTGATA TGACCGTGCG TAAAGAGTAC ATCACTGCAA AGTATGTCGA CCACCGCTTC 
TCCAGGAAGA CCTGTGCCTC TTCCTCAGCA AAGCTGAACG AATTGCTTGA GGCCATCAAG 
TCCAGGGACT TACTTGCATT AATCCAAGTC TATGCAGAGG GGGTGGAGTT AATGGAGCCA 
CTGCTGGAAC CTGGGCAGGA GCTCGGGGAG ACAGCCCTTC ATCTTGCCGT CAGGACCGCA 
GACCAGACAT CTCTCCATCT GGTTGATTTC CTTGTACAAA ACTGTGGGAA CCTGGATAAG 
CAAACGTCTG TGGGGAATAC AGTTCTGCAC TATTGCAGCA TGTACGGCAA GCCGGAGTGT 
CTGAAGCTGC TGCTTAGGAG CAAGCCCACC GTGGACATCG TTAACCAGAA TGGAGAAACT 
GCCTTGGATA TAGCAAAGAG ACTCAAAGCT ACCCAGTGTG AAGATCTGCT TTCCCAGGCT 
AAATCTGGAA AGTTCAACCC TCATGTCCAC GTAGAGTATG AGTGGAATCT TCGACAGGAC 
GAGATGGATG AGAGCGATGA CGATCTGGAT GACAAGCCAA GCCCGATCAA AAAGGAACGC 
TCGCCCAGAC CACAGAGCTT CTGCCACTCC TCCAGCATCT CGCCACAGGA CAAGCTGGCA 
CTGCCAGGGT TCAGCACTCC ACGGGACAAG CAGCGGCTCT CCTACGGAGC CTTCACCAAC 
CAGATCTTCG CCTCTACGAG CACAGATTTG CCCACATCAC CCACCAGTGA GGCTCCCCCT 
TTGCCACCTC GGAACGCCGG GAAAGGTCCA ACTGGCCCAC CTTCAACACT CCCTCTAGGC 
ACCCAGACCT CTAGTGGCAG CTCCACCCTA TCCAAGAAGA GGCCTCCTCC CCCACCACCA 
GGACACAAGA GAACCCTGTC TGACCCTCCC AGCCCACTAC CTCACGGGCC CCCAAACAAA 
GGCGCAATTC CTTGGGGTAA TGATGTGGGC CCATTATCTT CAAGTAAGAC GGCCAACAAG 
TTTGAGGGGC TGTCTCAGCA AGCAAGCACC AGTTCTGCTA AGACTGCCCT TGGCCCGAGA 
GTGCTTCCTA AACTACCTCA GAAAGTGGCA CTAAGGAAGA CGGAGACCAG CCATCATCTC 
TCCCTCGACA GAACCAACAT CCCACCTGAG ACTTTTCAGA AATCATCACA GTTGACAGAG 
TTACCCCAAA AGCCACCACT TGGAGAGCTG CCCCCGAAGC CTGTGGAACT GGCCCCCAAG 
CCCCAAGTTG GAGAGCTGCC ACCTAAGCCT GGAGAGCTAC CCCCTAAGCC CCAATTAGGT 
GACCTGCCCC CCAAGCCACA GCTCTCAGAC TTACCTCCCA AGCCACAGAT GAAGGACCTG 
CCCCCTAAGC CGCAGCTGGG GGATCTGCTG GCAAAGTCCC AGGCTGGCGA TGTCTCAGCC 
AAGGTGCAGC CACCCTCAGA GGTCACACAG AGGTCACACA CCGGGGATCT GTCTCCAAAT 
GTACAGTCCA GAGATGCCAT CCAGAAGCAA GCATCTGAAG ACTCCAACGA CCTCACACCC 
ACGCTGCCAG AGACACCCGT ACCACTGCCG AGAAAAATCA ATACGGGGAA AAATAAAGTG 
AGGCGGGTGA AGACCATTTA TGACTGCCAG GCAGATAATG ACGACGAACT CACATTTATT 
GAGGGGGAAG TGATCATTGT CACCGGGGAA GAGGACCAGG AGTGGTGGAT CGGGCATATC 
GAAGGACAGC CTGAAAGGAA GGGTGTCTTT CCAGTGTCCT TTGTCCACAT CCTGTCTGAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asap1 ( XM_006520394.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006520457.1 Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu50915
Clone ID Related Accession (Same CDS sequence)	XM_006520394.2
Accession Version	XM_006520394.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006520394.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAGAT GCATGCTTTG CATTCCTTTT GAATTCCCTT TGAGTCCCCA GGTCAGGCTC 
CAACACCATG TAAGGGATGG GCTTTCGACT GTGCTCATCC TGCCTTTCAT GTCTGCTCTG 
CTTCCCCAGC GTGAAGCTAG TGCCGAAGAG CTCCGCCAGC TTTATGAGTG GAGCAGGAAC 
CCACACTCAT TAAACACAAC AGGATGCCGG ACCAGATCTC CGTGTCCGAG TTCATCGCCG 
AGACCACCGA GGACTACAAC TCGCCCACCA CGTCCAGCTT CACCACGCGG CTGCACAACT 
GCAGGAACAC CGTCACGCTG CTGGAGGAGC GTGGTTGACG GTGGCAGCCG AGCAGTGGTG 
CAGATGACGC CAGCTGTGTT GCACTTGGGT GAGCCATACA CAAGTTCATT TGAGGCTCTA 
GACCAAGATA GAACAGCCCT ACAGAAAGTG AAGAAATCTG TAAAAGCAAT ATATAATTCC 
GGTCAAGATC ATGTACAAAA TGAAGAAAAC TATGCACAAG TTCTGGATAA GTTTGGAAGT 
AATTTTTTAA GCAGAGACAA CCCTGACCTT GGCACCGCTT TTGTCAAGTT TTCTACACTC 
ACAAAGGAAC TGTCCACGCT GCTGAAAAAT CTGCTCCAGG GTCTGAGCCA CAATGTGATC 
TTCACCTTGG ATTCCTTGTT AAAAGGAGAC TTGAAGGGGG TCAAAGGAGA TCTCAAGAAG 
CCATTTGACA AAGCCTGGAA AGATTATGAG ACGAAGTTTA CAAAAATTGA GAAGGAGAAA 
AGAGAGCACG CAAAACAGCA CGGGATGATC CGAACAGAGA TAACAGGCGC TGAGATTGCA 
GAAGAAATGG AAAAGGAACG GCGGCTTTTT CAGCTCCAGA TGTGTGAATA TCTCATTAAA 
GTAAATGAAA TCAAGACCAA AAAGGGTGTG GACCTGCTGC AGAACCTTAT AAAGTATTAC 
CATGCACAGT GCAATTTCTT TCAGGATGGC TTGAAAACGG CTGATAAACT GAAACAGTAC 
ATAGAAAAGC TGGCTGCTGA CTTATATAAT ATAAAGCAAA CCCAAGATGA AGAAAAAAAA 
CAGCTAACTG CACTCCGAGA TCTAATAAAA TCCTCTCTGC AACTTGATCC GAAAGAATCT 
AGAAGAGACT CTCAGAGCCG GCAAGGTGGG TACAGCATGC ATCAGCTCCA GGGCAACAAG 
GAGTACGGCA GTGAAAAGAA GGGCTTCCTG CTGAAGAAGA GTGATGGGAT CCGGAAAGTG 
TGGCAGAGAC GGAAGTGTGC GGTCAAGAAC GGGATCCTGA CCATCTCCCA CGCAACTTCC 
AACAGGCAGC CTGCTAAGTT AAACCTCCTC ACCTGCCAAG TGAAACCGAA TGCTGAGGAC 
AAGAAGTCTT TTGACCTGAT ATCACATAAT CGAACATATC ACTTTCAAGC AGAAGATGAG 
CAGGATTATA TAGCGTGGAT ATCAGTACTG ACGAATAGCA AAGAAGAGGC CCTAACCATG 
GCCTTCCGTG GTGAGCAGAG CACAGGGGAG AATAGCCTGG AGGACCTTAC CAAAGCCATC 
ATCGAGGATG TACAGCGGCT CCCTGGGAAT GACATCTGCT GTGACTGCGG CTCATCAGAA 
CCCACGTGGC TTTCAACCAA CTTGGGTATT TTGACCTGTA TAGAATGTTC CGGAATCCAT 
AGGGAAATGG GGGTTCATAT TTCTCGCATT CAGTCTTTGG AACTAGACAA ATTAGGAACT 
TCCGAACTCT TGCTGGCCAA GAATGTAGGA AACAATAGTT TTAATGATAT TATGGAAGCA 
AATTTACCCA GCCCTTCACC AAAGCCCACC CCTTCAAGTG ATATGACCGT GCGTAAAGAG 
TACATCACTG CAAAGTATGT CGACCACCGC TTCTCCAGGA AGACCTGTGC CTCTTCCTCA 
GCAAAGCTGA ACGAATTGCT TGAGGCCATC AAGTCCAGGG ACTTACTTGC ATTAATCCAA 
GTCTATGCAG AGGGGGTGGA GTTAATGGAG CCACTGCTGG AACCTGGGCA GGAGCTCGGG 
GAGACAGCCC TTCATCTTGC CGTCAGGACC GCAGACCAGA CATCTCTCCA TCTGGTTGAT 
TTCCTTGTAC AAAACTGTGG GAACCTGGAT AAGCAAACGT CTGTGGGGAA TACAGTTCTG 
CACTATTGCA GCATGTACGG CAAGCCGGAG TGTCTGAAGC TGCTGCTTAG GAGCAAGCCC 
ACCGTGGACA TCGTTAACCA GAATGGAGAA ACTGCCTTGG ATATAGCAAA GAGACTCAAA 
GCTACCCAGT GTGAAGATCT GCTTTCCCAG GCTAAATCTG GAAAGTTCAA CCCTCATGTC 
CACGTAGAGT ATGAGTGGAA TCTTCGACAG GACGAGATGG ATGAGAGCGA TGACGATCTG 
GATGACAAGC CAAGCCCGAT CAAAAAGGAA CGCTCGCCCA GACCACAGAG CTTCTGCCAC 
TCCTCCAGCA TCTCGCCACA GGACAAGCTG GCACTGCCAG GGTTCAGCAC TCCACGGGAC 
AAGCAGCGGC TCTCCTACGG AGCCTTCACC AACCAGATCT TCGCCTCTAC GAGCACAGAT 
TTGCCCACAT CACCCACCAG TGAGGCTCCC CCTTTGCCAC CTCGGAACGC CGGGAAAGGT 
CCAACTGGCC CACCTTCAAC ACTCCCTCTA GGCACCCAGA CCTCTAGTGG CAGCTCCACC 
CTATCCAAGA AGAGGCCTCC TCCCCCACCA CCAGGACACA AGAGAACCCT GTCTGACCCT 
CCCAGCCCAC TACCTCACGG GCCCCCAAAC AAAGGCGCAA TTCCTTGGGG TAATGATGTG 
GGCCCATTAT CTTCAAGTAA GACGGCCAAC AAGTTTGAGG GGCTGTCTCA GCAAGCAAGC 
ACCAGTTCTG CTAAGACTGC CCTTGGCCCG AGAGTGCTTC CTAAACTACC TCAGAAAGTG 
GCACTAAGGA AGACGGAGAC CAGCCATCAT CTCTCCCTCG ACAGAACCAA CATCCCACCT 
GAGACTTTTC AGAAATCATC ACAGTTGACA GAGTTACCCC AAAAGCCACC ACTTGGAGAG 
CTGCCCCCGA AGCCTGTGGA ACTGGCCCCC AAGCCCCAAG TTGGAGAGCT GCCACCTAAG 
CCTGGAGAGC TACCCCCTAA GCCCCAATTA GGTGACCTGC CCCCCAAGCC ACAGCTCTCA 
GACTTACCTC CCAAGCCACA GATGAAGGAC CTGCCCCCTA AGCCGCAGCT GGGGGATCTG 
CTGGCAAAGT CCCAGGCTGG CGATGTCTCA GCCAAGGTGC AGCCACCCTC AGAGGTCACA 
CAGAGGTCAC ACACCGGGGA TCTGTCTCCA AATGTACAGT CCAGAGATGC CATCCAGAAG 
CAAGCATCTG AAGACTCCAA CGACCTCACA CCCACGCTGC CAGAGACACC CGTACCACTG 
CCGAGAAAAA TCAATACGGG GAAAAATAAA GTGAGGCGGG TGAAGACCAT TTATGACTGC 
CAGGCAGATA ATGACGACGA ACTCACATTT ATTGAGGGGG AAGTGATCAT TGTCACCGGG 
GAAGAGGACC AGGAGTGGTG GATCGGGCAT ATCGAAGGAC AGCCTGAAAG GAAGGGTGTC 
TTTCCAGTGT CCTTTGTCCA CATCCTGTCT GACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006520457.1
CDS	16..3651
Translation	MSRCMLCIPFEFPLSPQVRLQHHVRDGLSTVLILPFMSALLPQREASAEELRQLYEWSRNPHSLNTTGCRTRSPCPSSSPRPPRTTTRPPRPASPRGCTTAGTPSRCWRSVVDGGSRAVVQMTPAVLHLGEPYTSSFEALDQDRTALQKVKKSVKAIYNSGQDHVQNEENYAQVLDKFGSNFLSRDNPDLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGVKGDLKKPFDKAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDPKESRRDSQSRQGGYSMHQLQGNKEYGSEKKGFLLKKSDGIRKVWQRRKCAVKNGILTISHATSNRQPAKLNLLTCQVKPNAEDKKSFDLISHNRTYHFQAEDEQDYIAWISVLTNSKEEALTMAFRGEQSTGENSLEDLTKAIIEDVQRLPGNDICCDCGSSEPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDKLGTSELLLAKNVGNNSFNDIMEANLPSPSPKPTPSSDMTVRKEYITAKYVDHRFSRKTCASSSAKLNELLEAIKSRDLLALIQVYAEGVELMEPLLEPGQELGETALHLAVRTADQTSLHLVDFLVQNCGNLDKQTSVGNTVLHYCSMYGKPECLKLLLRSKPTVDIVNQNGETALDIAKRLKATQCEDLLSQAKSGKFNPHVHVEYEWNLRQDEMDESDDDLDDKPSPIKKERSPRPQSFCHSSSISPQDKLALPGFSTPRDKQRLSYGAFTNQIFASTSTDLPTSPTSEAPPLPPRNAGKGPTGPPSTLPLGTQTSSGSSTLSKKRPPPPPPGHKRTLSDPPSPLPHGPPNKGAIPWGNDVGPLSSSKTANKFEGLSQQASTSSAKTALGPRVLPKLPQKVALRKTETSHHLSLDRTNIPPETFQKSSQLTELPQKPPLGELPPKPVELAPKPQVGELPPKPGELPPKPQLGDLPPKPQLSDLPPKPQMKDLPPKPQLGDLLAKSQAGDVSAKVQPPSEVTQRSHTGDLSPNVQSRDAIQKQASEDSNDLTPTLPETPVPLPRKINTGKNKVRRVKTIYDCQADNDDELTFIEGEVIIVTGEEDQEWWIGHIEGQPERKGVFPVSFVHILSD

Target ORF information:

RefSeq Version	XM_006520394.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006520394.2

ORF Insert Sequence:

ATGTCGAGAT GCATGCTTTG CATTCCTTTT GAATTCCCTT TGAGTCCCCA GGTCAGGCTC 
CAACACCATG TAAGGGATGG GCTTTCGACT GTGCTCATCC TGCCTTTCAT GTCTGCTCTG 
CTTCCCCAGC GTGAAGCTAG TGCCGAAGAG CTCCGCCAGC TTTATGAGTG GAGCAGGAAC 
CCACACTCAT TAAACACAAC AGGATGCCGG ACCAGATCTC CGTGTCCGAG TTCATCGCCG 
AGACCACCGA GGACTACAAC TCGCCCACCA CGTCCAGCTT CACCACGCGG CTGCACAACT 
GCAGGAACAC CGTCACGCTG CTGGAGGAGC GTGGTTGACG GTGGCAGCCG AGCAGTGGTG 
CAGATGACGC CAGCTGTGTT GCACTTGGGT GAGCCATACA CAAGTTCATT TGAGGCTCTA 
GACCAAGATA GAACAGCCCT ACAGAAAGTG AAGAAATCTG TAAAAGCAAT ATATAATTCC 
GGTCAAGATC ATGTACAAAA TGAAGAAAAC TATGCACAAG TTCTGGATAA GTTTGGAAGT 
AATTTTTTAA GCAGAGACAA CCCTGACCTT GGCACCGCTT TTGTCAAGTT TTCTACACTC 
ACAAAGGAAC TGTCCACGCT GCTGAAAAAT CTGCTCCAGG GTCTGAGCCA CAATGTGATC 
TTCACCTTGG ATTCCTTGTT AAAAGGAGAC TTGAAGGGGG TCAAAGGAGA TCTCAAGAAG 
CCATTTGACA AAGCCTGGAA AGATTATGAG ACGAAGTTTA CAAAAATTGA GAAGGAGAAA 
AGAGAGCACG CAAAACAGCA CGGGATGATC CGAACAGAGA TAACAGGCGC TGAGATTGCA 
GAAGAAATGG AAAAGGAACG GCGGCTTTTT CAGCTCCAGA TGTGTGAATA TCTCATTAAA 
GTAAATGAAA TCAAGACCAA AAAGGGTGTG GACCTGCTGC AGAACCTTAT AAAGTATTAC 
CATGCACAGT GCAATTTCTT TCAGGATGGC TTGAAAACGG CTGATAAACT GAAACAGTAC 
ATAGAAAAGC TGGCTGCTGA CTTATATAAT ATAAAGCAAA CCCAAGATGA AGAAAAAAAA 
CAGCTAACTG CACTCCGAGA TCTAATAAAA TCCTCTCTGC AACTTGATCC GAAAGAATCT 
AGAAGAGACT CTCAGAGCCG GCAAGGTGGG TACAGCATGC ATCAGCTCCA GGGCAACAAG 
GAGTACGGCA GTGAAAAGAA GGGCTTCCTG CTGAAGAAGA GTGATGGGAT CCGGAAAGTG 
TGGCAGAGAC GGAAGTGTGC GGTCAAGAAC GGGATCCTGA CCATCTCCCA CGCAACTTCC 
AACAGGCAGC CTGCTAAGTT AAACCTCCTC ACCTGCCAAG TGAAACCGAA TGCTGAGGAC 
AAGAAGTCTT TTGACCTGAT ATCACATAAT CGAACATATC ACTTTCAAGC AGAAGATGAG 
CAGGATTATA TAGCGTGGAT ATCAGTACTG ACGAATAGCA AAGAAGAGGC CCTAACCATG 
GCCTTCCGTG GTGAGCAGAG CACAGGGGAG AATAGCCTGG AGGACCTTAC CAAAGCCATC 
ATCGAGGATG TACAGCGGCT CCCTGGGAAT GACATCTGCT GTGACTGCGG CTCATCAGAA 
CCCACGTGGC TTTCAACCAA CTTGGGTATT TTGACCTGTA TAGAATGTTC CGGAATCCAT 
AGGGAAATGG GGGTTCATAT TTCTCGCATT CAGTCTTTGG AACTAGACAA ATTAGGAACT 
TCCGAACTCT TGCTGGCCAA GAATGTAGGA AACAATAGTT TTAATGATAT TATGGAAGCA 
AATTTACCCA GCCCTTCACC AAAGCCCACC CCTTCAAGTG ATATGACCGT GCGTAAAGAG 
TACATCACTG CAAAGTATGT CGACCACCGC TTCTCCAGGA AGACCTGTGC CTCTTCCTCA 
GCAAAGCTGA ACGAATTGCT TGAGGCCATC AAGTCCAGGG ACTTACTTGC ATTAATCCAA 
GTCTATGCAG AGGGGGTGGA GTTAATGGAG CCACTGCTGG AACCTGGGCA GGAGCTCGGG 
GAGACAGCCC TTCATCTTGC CGTCAGGACC GCAGACCAGA CATCTCTCCA TCTGGTTGAT 
TTCCTTGTAC AAAACTGTGG GAACCTGGAT AAGCAAACGT CTGTGGGGAA TACAGTTCTG 
CACTATTGCA GCATGTACGG CAAGCCGGAG TGTCTGAAGC TGCTGCTTAG GAGCAAGCCC 
ACCGTGGACA TCGTTAACCA GAATGGAGAA ACTGCCTTGG ATATAGCAAA GAGACTCAAA 
GCTACCCAGT GTGAAGATCT GCTTTCCCAG GCTAAATCTG GAAAGTTCAA CCCTCATGTC 
CACGTAGAGT ATGAGTGGAA TCTTCGACAG GACGAGATGG ATGAGAGCGA TGACGATCTG 
GATGACAAGC CAAGCCCGAT CAAAAAGGAA CGCTCGCCCA GACCACAGAG CTTCTGCCAC 
TCCTCCAGCA TCTCGCCACA GGACAAGCTG GCACTGCCAG GGTTCAGCAC TCCACGGGAC 
AAGCAGCGGC TCTCCTACGG AGCCTTCACC AACCAGATCT TCGCCTCTAC GAGCACAGAT 
TTGCCCACAT CACCCACCAG TGAGGCTCCC CCTTTGCCAC CTCGGAACGC CGGGAAAGGT 
CCAACTGGCC CACCTTCAAC ACTCCCTCTA GGCACCCAGA CCTCTAGTGG CAGCTCCACC 
CTATCCAAGA AGAGGCCTCC TCCCCCACCA CCAGGACACA AGAGAACCCT GTCTGACCCT 
CCCAGCCCAC TACCTCACGG GCCCCCAAAC AAAGGCGCAA TTCCTTGGGG TAATGATGTG 
GGCCCATTAT CTTCAAGTAA GACGGCCAAC AAGTTTGAGG GGCTGTCTCA GCAAGCAAGC 
ACCAGTTCTG CTAAGACTGC CCTTGGCCCG AGAGTGCTTC CTAAACTACC TCAGAAAGTG 
GCACTAAGGA AGACGGAGAC CAGCCATCAT CTCTCCCTCG ACAGAACCAA CATCCCACCT 
GAGACTTTTC AGAAATCATC ACAGTTGACA GAGTTACCCC AAAAGCCACC ACTTGGAGAG 
CTGCCCCCGA AGCCTGTGGA ACTGGCCCCC AAGCCCCAAG TTGGAGAGCT GCCACCTAAG 
CCTGGAGAGC TACCCCCTAA GCCCCAATTA GGTGACCTGC CCCCCAAGCC ACAGCTCTCA 
GACTTACCTC CCAAGCCACA GATGAAGGAC CTGCCCCCTA AGCCGCAGCT GGGGGATCTG 
CTGGCAAAGT CCCAGGCTGG CGATGTCTCA GCCAAGGTGC AGCCACCCTC AGAGGTCACA 
CAGAGGTCAC ACACCGGGGA TCTGTCTCCA AATGTACAGT CCAGAGATGC CATCCAGAAG 
CAAGCATCTG AAGACTCCAA CGACCTCACA CCCACGCTGC CAGAGACACC CGTACCACTG 
CCGAGAAAAA TCAATACGGG GAAAAATAAA GTGAGGCGGG TGAAGACCAT TTATGACTGC 
CAGGCAGATA ATGACGACGA ACTCACATTT ATTGAGGGGG AAGTGATCAT TGTCACCGGG 
GAAGAGGACC AGGAGTGGTG GATCGGGCAT ATCGAAGGAC AGCCTGAAAG GAAGGGTGTC 
TTTCCAGTGT CCTTTGTCCA CATCCTGTCT GACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asap1 ( XM_006520395.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006520458.1 Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X4, mRNA.

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CloneID	OMu50916
Clone ID Related Accession (Same CDS sequence)	XM_006520395.2
Accession Version	XM_006520395.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006520395.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAGAT GCATGCTTTG CATTCCTTTT GAATTCCCTT TGAGTCCCCA GGTCAGGCTC 
CAACACCATG TAAGGGATGG GCTTTCGACT GTGCTCATCC TGCCTTTCAT GTCTGCTCTG 
CTTCCCCAGC GTGAAGCTAG TGCCGAAGAG CTCCGCCAGC TTTATGAGTG GAGCAGGAAC 
CCACACTCAT TAAACACAAC AGGATGCCGG ACCAGATCTC CGTGTCCGAG TTCATCGCCG 
AGACCACCGA GGACTACAAC TCGCCCACCA CGTCCAGCTT CACCACGCGG CTGCACAACT 
GCAGGAACAC CGTCACGCTG CTGGAGGAGC GTGGTTGACG GTGGCAGCCG AGCAGTGGTG 
CAGATGACGC CAGCTGTGTT GCACTTGGGT GAGCCATACA CAAGTTCATT TGAGGCTCTA 
GACCAAGATA GAACAGCCCT ACAGAAAGTG AAGAAATCTG TAAAAGCAAT ATATAATTCC 
GGTCAAGATC ATGTACAAAA TGAAGAAAAC TATGCACAAG TTCTGGATAA GTTTGGAAGT 
AATTTTTTAA GCAGAGACAA CCCTGACCTT GGCACCGCTT TTGTCAAGTT TTCTACACTC 
ACAAAGGAAC TGTCCACGCT GCTGAAAAAT CTGCTCCAGG GTCTGAGCCA CAATGTGATC 
TTCACCTTGG ATTCCTTGTT AAAAGGAGAC TTGAAGGGGG TCAAAGGAGA TCTCAAGAAG 
CCATTTGACA AAGCCTGGAA AGATTATGAG ACGAAGTTTA CAAAAATTGA GAAGGAGAAA 
AGAGAGCACG CAAAACAGCA CGGGATGATC CGAACAGAGA TAACAGGCGC TGAGATTGCA 
GAAGAAATGG AAAAGGAACG GCGGCTTTTT CAGCTCCAGA TGTGTGAATA TCTCATTAAA 
GTAAATGAAA TCAAGACCAA AAAGGGTGTG GACCTGCTGC AGAACCTTAT AAAGTATTAC 
CATGCACAGT GCAATTTCTT TCAGGATGGC TTGAAAACGG CTGATAAACT GAAACAGTAC 
ATAGAAAAGC TGGCTGCTGA CTTATATAAT ATAAAGCAAA CCCAAGATGA AGAAAAAAAA 
CAGCTAACTG CACTCCGAGA TCTAATAAAA TCCTCTCTGC AACTTGATCC GAAAGAAGAC 
TCTCAGAGCC GGCAAGGTGG GTACAGCATG CATCAGCTCC AGGGCAACAA GGAGTACGGC 
AGTGAAAAGA AGGGCTTCCT GCTGAAGAAG AGTGATGGGA TCCGGAAAGT GTGGCAGAGA 
CGGAAGTGTG CGGTCAAGAA CGGGATCCTG ACCATCTCCC ACGCAACTTC CAACAGGCAG 
CCTGCTAAGT TAAACCTCCT CACCTGCCAA GTGAAACCGA ATGCTGAGGA CAAGAAGTCT 
TTTGACCTGA TATCACATAA TCGAACATAT CACTTTCAAG CAGAAGATGA GCAGGATTAT 
ATAGCGTGGA TATCAGTACT GACGAATAGC AAAGAAGAGG CCCTAACCAT GGCCTTCCGT 
GGTGAGCAGA GCACAGGGGA GAATAGCCTG GAGGACCTTA CCAAAGCCAT CATCGAGGAT 
GTACAGCGGC TCCCTGGGAA TGACATCTGC TGTGACTGCG GCTCATCAGA ACCCACGTGG 
CTTTCAACCA ACTTGGGTAT TTTGACCTGT ATAGAATGTT CCGGAATCCA TAGGGAAATG 
GGGGTTCATA TTTCTCGCAT TCAGTCTTTG GAACTAGACA AATTAGGAAC TTCCGAACTC 
TTGCTGGCCA AGAATGTAGG AAACAATAGT TTTAATGATA TTATGGAAGC AAATTTACCC 
AGCCCTTCAC CAAAGCCCAC CCCTTCAAGT GATATGACCG TGCGTAAAGA GTACATCACT 
GCAAAGTATG TCGACCACCG CTTCTCCAGG AAGACCTGTG CCTCTTCCTC AGCAAAGCTG 
AACGAATTGC TTGAGGCCAT CAAGTCCAGG GACTTACTTG CATTAATCCA AGTCTATGCA 
GAGGGGGTGG AGTTAATGGA GCCACTGCTG GAACCTGGGC AGGAGCTCGG GGAGACAGCC 
CTTCATCTTG CCGTCAGGAC CGCAGACCAG ACATCTCTCC ATCTGGTTGA TTTCCTTGTA 
CAAAACTGTG GGAACCTGGA TAAGCAAACG TCTGTGGGGA ATACAGTTCT GCACTATTGC 
AGCATGTACG GCAAGCCGGA GTGTCTGAAG CTGCTGCTTA GGAGCAAGCC CACCGTGGAC 
ATCGTTAACC AGAATGGAGA AACTGCCTTG GATATAGCAA AGAGACTCAA AGCTACCCAG 
TGTGAAGATC TGCTTTCCCA GGCTAAATCT GGAAAGTTCA ACCCTCATGT CCACGTAGAG 
TATGAGTGGA ATCTTCGACA GGACGAGATG GATGAGAGCG ATGACGATCT GGATGACAAG 
CCAAGCCCGA TCAAAAAGGA ACGCTCGCCC AGACCACAGA GCTTCTGCCA CTCCTCCAGC 
ATCTCGCCAC AGGACAAGCT GGCACTGCCA GGGTTCAGCA CTCCACGGGA CAAGCAGCGG 
CTCTCCTACG GAGCCTTCAC CAACCAGATC TTCGCCTCTA CGAGCACAGA TTTGCCCACA 
TCACCCACCA GTGAGGCTCC CCCTTTGCCA CCTCGGAACG CCGGGAAAGG TCCAACTGGC 
CCACCTTCAA CACTCCCTCT AGGCACCCAG ACCTCTAGTG GCAGCTCCAC CCTATCCAAG 
AAGAGGCCTC CTCCCCCACC ACCAGGACAC AAGAGAACCC TGTCTGACCC TCCCAGCCCA 
CTACCTCACG GGCCCCCAAA CAAAGGCGCA ATTCCTTGGG GTAATGATGT GGGCCCATTA 
TCTTCAAGTA AGACGGCCAA CAAGTTTGAG GGGCTGTCTC AGCAAGCAAG CACCAGTTCT 
GCTAAGACTG CCCTTGGCCC GAGAGTGCTT CCTAAACTAC CTCAGAAAGT GGCACTAAGG 
AAGACGGAGA CCAGCCATCA TCTCTCCCTC GACAGAACCA ACATCCCACC TGAGACTTTT 
CAGAAATCAT CACAGTTGAC AGAGTTACCC CAAAAGCCAC CACTTGGAGA GCTGCCCCCG 
AAGCCTGTGG AACTGGCCCC CAAGCCCCAA GTTGGAGAGC TGCCACCTAA GCCTGGAGAG 
CTACCCCCTA AGCCCCAATT AGGTGACCTG CCCCCCAAGC CACAGCTCTC AGACTTACCT 
CCCAAGCCAC AGATGAAGGA CCTGCCCCCT AAGCCGCAGC TGGGGGATCT GCTGGCAAAG 
TCCCAGGCTG GCGATGTCTC AGCCAAGGTG CAGCCACCCT CAGAGGTCAC ACAGAGGTCA 
CACACCGGGG ATCTGTCTCC AAATGTACAG TCCAGAGATG CCATCCAGAA GCAAGCATCT 
GAAGACTCCA ACGACCTCAC ACCCACGCTG CCAGAGACAC CCGTACCACT GCCGAGAAAA 
ATCAATACGG GGAAAAATAA AGTGAGGCGG GTGAAGACCA TTTATGACTG CCAGGCAGAT 
AATGACGACG AACTCACATT TATTGAGGGG GAAGTGATCA TTGTCACCGG GGAAGAGGAC 
CAGGAGTGGT GGATCGGGCA TATCGAAGGA CAGCCTGAAA GGAAGGGTGT CTTTCCAGTG 
TCCTTTGTCC ACATCCTGTC TGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006520458.1
CDS	16..3642
Translation	MSRCMLCIPFEFPLSPQVRLQHHVRDGLSTVLILPFMSALLPQREASAEELRQLYEWSRNPHSLNTTGCRTRSPCPSSSPRPPRTTTRPPRPASPRGCTTAGTPSRCWRSVVDGGSRAVVQMTPAVLHLGEPYTSSFEALDQDRTALQKVKKSVKAIYNSGQDHVQNEENYAQVLDKFGSNFLSRDNPDLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGVKGDLKKPFDKAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDPKEDSQSRQGGYSMHQLQGNKEYGSEKKGFLLKKSDGIRKVWQRRKCAVKNGILTISHATSNRQPAKLNLLTCQVKPNAEDKKSFDLISHNRTYHFQAEDEQDYIAWISVLTNSKEEALTMAFRGEQSTGENSLEDLTKAIIEDVQRLPGNDICCDCGSSEPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDKLGTSELLLAKNVGNNSFNDIMEANLPSPSPKPTPSSDMTVRKEYITAKYVDHRFSRKTCASSSAKLNELLEAIKSRDLLALIQVYAEGVELMEPLLEPGQELGETALHLAVRTADQTSLHLVDFLVQNCGNLDKQTSVGNTVLHYCSMYGKPECLKLLLRSKPTVDIVNQNGETALDIAKRLKATQCEDLLSQAKSGKFNPHVHVEYEWNLRQDEMDESDDDLDDKPSPIKKERSPRPQSFCHSSSISPQDKLALPGFSTPRDKQRLSYGAFTNQIFASTSTDLPTSPTSEAPPLPPRNAGKGPTGPPSTLPLGTQTSSGSSTLSKKRPPPPPPGHKRTLSDPPSPLPHGPPNKGAIPWGNDVGPLSSSKTANKFEGLSQQASTSSAKTALGPRVLPKLPQKVALRKTETSHHLSLDRTNIPPETFQKSSQLTELPQKPPLGELPPKPVELAPKPQVGELPPKPGELPPKPQLGDLPPKPQLSDLPPKPQMKDLPPKPQLGDLLAKSQAGDVSAKVQPPSEVTQRSHTGDLSPNVQSRDAIQKQASEDSNDLTPTLPETPVPLPRKINTGKNKVRRVKTIYDCQADNDDELTFIEGEVIIVTGEEDQEWWIGHIEGQPERKGVFPVSFVHILSD

Target ORF information:

RefSeq Version	XM_006520395.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006520395.2

ORF Insert Sequence:

ATGTCGAGAT GCATGCTTTG CATTCCTTTT GAATTCCCTT TGAGTCCCCA GGTCAGGCTC 
CAACACCATG TAAGGGATGG GCTTTCGACT GTGCTCATCC TGCCTTTCAT GTCTGCTCTG 
CTTCCCCAGC GTGAAGCTAG TGCCGAAGAG CTCCGCCAGC TTTATGAGTG GAGCAGGAAC 
CCACACTCAT TAAACACAAC AGGATGCCGG ACCAGATCTC CGTGTCCGAG TTCATCGCCG 
AGACCACCGA GGACTACAAC TCGCCCACCA CGTCCAGCTT CACCACGCGG CTGCACAACT 
GCAGGAACAC CGTCACGCTG CTGGAGGAGC GTGGTTGACG GTGGCAGCCG AGCAGTGGTG 
CAGATGACGC CAGCTGTGTT GCACTTGGGT GAGCCATACA CAAGTTCATT TGAGGCTCTA 
GACCAAGATA GAACAGCCCT ACAGAAAGTG AAGAAATCTG TAAAAGCAAT ATATAATTCC 
GGTCAAGATC ATGTACAAAA TGAAGAAAAC TATGCACAAG TTCTGGATAA GTTTGGAAGT 
AATTTTTTAA GCAGAGACAA CCCTGACCTT GGCACCGCTT TTGTCAAGTT TTCTACACTC 
ACAAAGGAAC TGTCCACGCT GCTGAAAAAT CTGCTCCAGG GTCTGAGCCA CAATGTGATC 
TTCACCTTGG ATTCCTTGTT AAAAGGAGAC TTGAAGGGGG TCAAAGGAGA TCTCAAGAAG 
CCATTTGACA AAGCCTGGAA AGATTATGAG ACGAAGTTTA CAAAAATTGA GAAGGAGAAA 
AGAGAGCACG CAAAACAGCA CGGGATGATC CGAACAGAGA TAACAGGCGC TGAGATTGCA 
GAAGAAATGG AAAAGGAACG GCGGCTTTTT CAGCTCCAGA TGTGTGAATA TCTCATTAAA 
GTAAATGAAA TCAAGACCAA AAAGGGTGTG GACCTGCTGC AGAACCTTAT AAAGTATTAC 
CATGCACAGT GCAATTTCTT TCAGGATGGC TTGAAAACGG CTGATAAACT GAAACAGTAC 
ATAGAAAAGC TGGCTGCTGA CTTATATAAT ATAAAGCAAA CCCAAGATGA AGAAAAAAAA 
CAGCTAACTG CACTCCGAGA TCTAATAAAA TCCTCTCTGC AACTTGATCC GAAAGAAGAC 
TCTCAGAGCC GGCAAGGTGG GTACAGCATG CATCAGCTCC AGGGCAACAA GGAGTACGGC 
AGTGAAAAGA AGGGCTTCCT GCTGAAGAAG AGTGATGGGA TCCGGAAAGT GTGGCAGAGA 
CGGAAGTGTG CGGTCAAGAA CGGGATCCTG ACCATCTCCC ACGCAACTTC CAACAGGCAG 
CCTGCTAAGT TAAACCTCCT CACCTGCCAA GTGAAACCGA ATGCTGAGGA CAAGAAGTCT 
TTTGACCTGA TATCACATAA TCGAACATAT CACTTTCAAG CAGAAGATGA GCAGGATTAT 
ATAGCGTGGA TATCAGTACT GACGAATAGC AAAGAAGAGG CCCTAACCAT GGCCTTCCGT 
GGTGAGCAGA GCACAGGGGA GAATAGCCTG GAGGACCTTA CCAAAGCCAT CATCGAGGAT 
GTACAGCGGC TCCCTGGGAA TGACATCTGC TGTGACTGCG GCTCATCAGA ACCCACGTGG 
CTTTCAACCA ACTTGGGTAT TTTGACCTGT ATAGAATGTT CCGGAATCCA TAGGGAAATG 
GGGGTTCATA TTTCTCGCAT TCAGTCTTTG GAACTAGACA AATTAGGAAC TTCCGAACTC 
TTGCTGGCCA AGAATGTAGG AAACAATAGT TTTAATGATA TTATGGAAGC AAATTTACCC 
AGCCCTTCAC CAAAGCCCAC CCCTTCAAGT GATATGACCG TGCGTAAAGA GTACATCACT 
GCAAAGTATG TCGACCACCG CTTCTCCAGG AAGACCTGTG CCTCTTCCTC AGCAAAGCTG 
AACGAATTGC TTGAGGCCAT CAAGTCCAGG GACTTACTTG CATTAATCCA AGTCTATGCA 
GAGGGGGTGG AGTTAATGGA GCCACTGCTG GAACCTGGGC AGGAGCTCGG GGAGACAGCC 
CTTCATCTTG CCGTCAGGAC CGCAGACCAG ACATCTCTCC ATCTGGTTGA TTTCCTTGTA 
CAAAACTGTG GGAACCTGGA TAAGCAAACG TCTGTGGGGA ATACAGTTCT GCACTATTGC 
AGCATGTACG GCAAGCCGGA GTGTCTGAAG CTGCTGCTTA GGAGCAAGCC CACCGTGGAC 
ATCGTTAACC AGAATGGAGA AACTGCCTTG GATATAGCAA AGAGACTCAA AGCTACCCAG 
TGTGAAGATC TGCTTTCCCA GGCTAAATCT GGAAAGTTCA ACCCTCATGT CCACGTAGAG 
TATGAGTGGA ATCTTCGACA GGACGAGATG GATGAGAGCG ATGACGATCT GGATGACAAG 
CCAAGCCCGA TCAAAAAGGA ACGCTCGCCC AGACCACAGA GCTTCTGCCA CTCCTCCAGC 
ATCTCGCCAC AGGACAAGCT GGCACTGCCA GGGTTCAGCA CTCCACGGGA CAAGCAGCGG 
CTCTCCTACG GAGCCTTCAC CAACCAGATC TTCGCCTCTA CGAGCACAGA TTTGCCCACA 
TCACCCACCA GTGAGGCTCC CCCTTTGCCA CCTCGGAACG CCGGGAAAGG TCCAACTGGC 
CCACCTTCAA CACTCCCTCT AGGCACCCAG ACCTCTAGTG GCAGCTCCAC CCTATCCAAG 
AAGAGGCCTC CTCCCCCACC ACCAGGACAC AAGAGAACCC TGTCTGACCC TCCCAGCCCA 
CTACCTCACG GGCCCCCAAA CAAAGGCGCA ATTCCTTGGG GTAATGATGT GGGCCCATTA 
TCTTCAAGTA AGACGGCCAA CAAGTTTGAG GGGCTGTCTC AGCAAGCAAG CACCAGTTCT 
GCTAAGACTG CCCTTGGCCC GAGAGTGCTT CCTAAACTAC CTCAGAAAGT GGCACTAAGG 
AAGACGGAGA CCAGCCATCA TCTCTCCCTC GACAGAACCA ACATCCCACC TGAGACTTTT 
CAGAAATCAT CACAGTTGAC AGAGTTACCC CAAAAGCCAC CACTTGGAGA GCTGCCCCCG 
AAGCCTGTGG AACTGGCCCC CAAGCCCCAA GTTGGAGAGC TGCCACCTAA GCCTGGAGAG 
CTACCCCCTA AGCCCCAATT AGGTGACCTG CCCCCCAAGC CACAGCTCTC AGACTTACCT 
CCCAAGCCAC AGATGAAGGA CCTGCCCCCT AAGCCGCAGC TGGGGGATCT GCTGGCAAAG 
TCCCAGGCTG GCGATGTCTC AGCCAAGGTG CAGCCACCCT CAGAGGTCAC ACAGAGGTCA 
CACACCGGGG ATCTGTCTCC AAATGTACAG TCCAGAGATG CCATCCAGAA GCAAGCATCT 
GAAGACTCCA ACGACCTCAC ACCCACGCTG CCAGAGACAC CCGTACCACT GCCGAGAAAA 
ATCAATACGG GGAAAAATAA AGTGAGGCGG GTGAAGACCA TTTATGACTG CCAGGCAGAT 
AATGACGACG AACTCACATT TATTGAGGGG GAAGTGATCA TTGTCACCGG GGAAGAGGAC 
CAGGAGTGGT GGATCGGGCA TATCGAAGGA CAGCCTGAAA GGAAGGGTGT CTTTCCAGTG 
TCCTTTGTCC ACATCCTGTC TGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asap1 ( XM_006520396.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006520459.1 Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X5, mRNA.

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CloneID	OMu50917
Clone ID Related Accession (Same CDS sequence)	XM_006520396.3 , XM_006520396.4
Accession Version	XM_006520396.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006520396.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCCA GTCATACGCT TTCAAGGGCT CCTGGAGGCC AATCCCAAGG ACTAGGATGC 
CGGACCAGAT CTCCGTGTCC GAGTTCATCG CCGAGACCAC CGAGGACTAC AACTCGCCCA 
CCACGTCCAG CTTCACCACG CGGCTGCACA ACTGCAGGAA CACCGTCACG CTGCTGGAGG 
AGCGTGGTTG ACGGTGGCAG CCGAGCAGTG GTGCAGATGA CGCCAGCTGT GTTGCACTTG 
GGTGAGCCAT ACACAAGTTC ATTTGAGGCT CTAGACCAAG ATAGAACAGC CCTACAGAAA 
GTGAAGAAAT CTGTAAAAGC AATATATAAT TCCGGTCAAG ATCATGTACA AAATGAAGAA 
AACTATGCAC AAGTTCTGGA TAAGTTTGGA AGTAATTTTT TAAGCAGAGA CAACCCTGAC 
CTTGGCACCG CTTTTGTCAA GTTTTCTACA CTCACAAAGG AACTGTCCAC GCTGCTGAAA 
AATCTGCTCC AGGGTCTGAG CCACAATGTG ATCTTCACCT TGGATTCCTT GTTAAAAGGA 
GACTTGAAGG GGGTCAAAGG AGATCTCAAG AAGCCATTTG ACAAAGCCTG GAAAGATTAT 
GAGACGAAGT TTACAAAAAT TGAGAAGGAG AAAAGAGAGC ACGCAAAACA GCACGGGATG 
ATCCGAACAG AGATAACAGG CGCTGAGATT GCAGAAGAAA TGGAAAAGGA ACGGCGGCTT 
TTTCAGCTCC AGATGTGTGA ATATCTCATT AAAGTAAATG AAATCAAGAC CAAAAAGGGT 
GTGGACCTGC TGCAGAACCT TATAAAGTAT TACCATGCAC AGTGCAATTT CTTTCAGGAT 
GGCTTGAAAA CGGCTGATAA ACTGAAACAG TACATAGAAA AGCTGGCTGC TGACTTATAT 
AATATAAAGC AAACCCAAGA TGAAGAAAAA AAACAGCTAA CTGCACTCCG AGATCTAATA 
AAATCCTCTC TGCAACTTGA TCCGAAAGAA GTAGGTGGTT TATATGTTGC CAGCAGGGCT 
AACAGTTCTA GAAGAGACTC TCAGAGCCGG CAAGGTGGGT ACAGCATGCA TCAGCTCCAG 
GGCAACAAGG AGTACGGCAG TGAAAAGAAG GGCTTCCTGC TGAAGAAGAG TGATGGGATC 
CGGAAAGTGT GGCAGAGACG GAAGTGTGCG GTCAAGAACG GGATCCTGAC CATCTCCCAC 
GCAACTTCCA ACAGGCAGCC TGCTAAGTTA AACCTCCTCA CCTGCCAAGT GAAACCGAAT 
GCTGAGGACA AGAAGTCTTT TGACCTGATA TCACATAATC GAACATATCA CTTTCAAGCA 
GAAGATGAGC AGGATTATAT AGCGTGGATA TCAGTACTGA CGAATAGCAA AGAAGAGGCC 
CTAACCATGG CCTTCCGTGG TGAGCAGAGC ACAGGGGAGA ATAGCCTGGA GGACCTTACC 
AAAGCCATCA TCGAGGATGT ACAGCGGCTC CCTGGGAATG ACATCTGCTG TGACTGCGGC 
TCATCAGAAC CCACGTGGCT TTCAACCAAC TTGGGTATTT TGACCTGTAT AGAATGTTCC 
GGAATCCATA GGGAAATGGG GGTTCATATT TCTCGCATTC AGTCTTTGGA ACTAGACAAA 
TTAGGAACTT CCGAACTCTT GCTGGCCAAG AATGTAGGAA ACAATAGTTT TAATGATATT 
ATGGAAGCAA ATTTACCCAG CCCTTCACCA AAGCCCACCC CTTCAAGTGA TATGACCGTG 
CGTAAAGAGT ACATCACTGC AAAGTATGTC GACCACCGCT TCTCCAGGAA GACCTGTGCC 
TCTTCCTCAG CAAAGCTGAA CGAATTGCTT GAGGCCATCA AGTCCAGGGA CTTACTTGCA 
TTAATCCAAG TCTATGCAGA GGGGGTGGAG TTAATGGAGC CACTGCTGGA ACCTGGGCAG 
GAGCTCGGGG AGACAGCCCT TCATCTTGCC GTCAGGACCG CAGACCAGAC ATCTCTCCAT 
CTGGTTGATT TCCTTGTACA AAACTGTGGG AACCTGGATA AGCAAACGTC TGTGGGGAAT 
ACAGTTCTGC ACTATTGCAG CATGTACGGC AAGCCGGAGT GTCTGAAGCT GCTGCTTAGG 
AGCAAGCCCA CCGTGGACAT CGTTAACCAG AATGGAGAAA CTGCCTTGGA TATAGCAAAG 
AGACTCAAAG CTACCCAGTG TGAAGATCTG CTTTCCCAGG CTAAATCTGG AAAGTTCAAC 
CCTCATGTCC ACGTAGAGTA TGAGTGGAAT CTTCGACAGG ACGAGATGGA TGAGAGCGAT 
GACGATCTGG ATGACAAGCC AAGCCCGATC AAAAAGGAAC GCTCGCCCAG ACCACAGAGC 
TTCTGCCACT CCTCCAGCAT CTCGCCACAG GACAAGCTGG CACTGCCAGG GTTCAGCACT 
CCACGGGACA AGCAGCGGCT CTCCTACGGA GCCTTCACCA ACCAGATCTT CGCCTCTACG 
AGCACAGATT TGCCCACATC ACCCACCAGT GAGGCTCCCC CTTTGCCACC TCGGAACGCC 
GGGAAAGGTC CAACTGGCCC ACCTTCAACA CTCCCTCTAG GCACCCAGAC CTCTAGTGGC 
AGCTCCACCC TATCCAAGAA GAGGCCTCCT CCCCCACCAC CAGGACACAA GAGAACCCTG 
TCTGACCCTC CCAGCCCACT ACCTCACGGG CCCCCAAACA AAGGCGCAAT TCCTTGGGGT 
AATGATGTGG GCCCATTATC TTCAAGTAAG ACGGCCAACA AGTTTGAGGG GCTGTCTCAG 
CAAGCAAGCA CCAGTTCTGC TAAGACTGCC CTTGGCCCGA GAGTGCTTCC TAAACTACCT 
CAGAAAGTGG CACTAAGGAA GACGGAGACC AGCCATCATC TCTCCCTCGA CAGAACCAAC 
ATCCCACCTG AGACTTTTCA GAAATCATCA CAGTTGACAG AGTTACCCCA AAAGCCACCA 
CTTGGAGAGC TGCCCCCGAA GCCTGTGGAA CTGGCCCCCA AGCCCCAAGT TGGAGAGCTG 
CCACCTAAGC CTGGAGAGCT ACCCCCTAAG CCCCAATTAG GTGACCTGCC CCCCAAGCCA 
CAGCTCTCAG ACTTACCTCC CAAGCCACAG ATGAAGGACC TGCCCCCTAA GCCGCAGCTG 
GGGGATCTGC TGGCAAAGTC CCAGGCTGGC GATGTCTCAG CCAAGGTGCA GCCACCCTCA 
GAGGTCACAC AGAGGTCACA CACCGGGGAT CTGTCTCCAA ATGTACAGTC CAGAGATGCC 
ATCCAGAAGC AAGCATCTGA AGACTCCAAC GACCTCACAC CCACGCTGCC AGAGACACCC 
GTACCACTGC CGAGAAAAAT CAATACGGGG AAAAATAAAG TGAGGCGGGT GAAGACCATT 
TATGACTGCC AGGCAGATAA TGACGACGAA CTCACATTTA TTGAGGGGGA AGTGATCATT 
GTCACCGGGG AAGAGGACCA GGAGTGGTGG ATCGGGCATA TCGAAGGACA GCCTGAAAGG 
AAGGGTGTCT TTCCAGTGTC CTTTGTCCAC ATCCTGTCTG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006520459.1
CDS	250..3774
Translation	MEASHTLSRAPGGQSQGLGCRTRSPCPSSSPRPPRTTTRPPRPASPRGCTTAGTPSRCWRSVVDGGSRAVVQMTPAVLHLGEPYTSSFEALDQDRTALQKVKKSVKAIYNSGQDHVQNEENYAQVLDKFGSNFLSRDNPDLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGVKGDLKKPFDKAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDPKEVGGLYVASRANSSRRDSQSRQGGYSMHQLQGNKEYGSEKKGFLLKKSDGIRKVWQRRKCAVKNGILTISHATSNRQPAKLNLLTCQVKPNAEDKKSFDLISHNRTYHFQAEDEQDYIAWISVLTNSKEEALTMAFRGEQSTGENSLEDLTKAIIEDVQRLPGNDICCDCGSSEPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDKLGTSELLLAKNVGNNSFNDIMEANLPSPSPKPTPSSDMTVRKEYITAKYVDHRFSRKTCASSSAKLNELLEAIKSRDLLALIQVYAEGVELMEPLLEPGQELGETALHLAVRTADQTSLHLVDFLVQNCGNLDKQTSVGNTVLHYCSMYGKPECLKLLLRSKPTVDIVNQNGETALDIAKRLKATQCEDLLSQAKSGKFNPHVHVEYEWNLRQDEMDESDDDLDDKPSPIKKERSPRPQSFCHSSSISPQDKLALPGFSTPRDKQRLSYGAFTNQIFASTSTDLPTSPTSEAPPLPPRNAGKGPTGPPSTLPLGTQTSSGSSTLSKKRPPPPPPGHKRTLSDPPSPLPHGPPNKGAIPWGNDVGPLSSSKTANKFEGLSQQASTSSAKTALGPRVLPKLPQKVALRKTETSHHLSLDRTNIPPETFQKSSQLTELPQKPPLGELPPKPVELAPKPQVGELPPKPGELPPKPQLGDLPPKPQLSDLPPKPQMKDLPPKPQLGDLLAKSQAGDVSAKVQPPSEVTQRSHTGDLSPNVQSRDAIQKQASEDSNDLTPTLPETPVPLPRKINTGKNKVRRVKTIYDCQADNDDELTFIEGEVIIVTGEEDQEWWIGHIEGQPERKGVFPVSFVHILSD

Target ORF information:

RefSeq Version	XM_006520396.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006520396.3

ORF Insert Sequence:

ATGGAGGCCA GTCATACGCT TTCAAGGGCT CCTGGAGGCC AATCCCAAGG ACTAGGATGC 
CGGACCAGAT CTCCGTGTCC GAGTTCATCG CCGAGACCAC CGAGGACTAC AACTCGCCCA 
CCACGTCCAG CTTCACCACG CGGCTGCACA ACTGCAGGAA CACCGTCACG CTGCTGGAGG 
AGCGTGGTTG ACGGTGGCAG CCGAGCAGTG GTGCAGATGA CGCCAGCTGT GTTGCACTTG 
GGTGAGCCAT ACACAAGTTC ATTTGAGGCT CTAGACCAAG ATAGAACAGC CCTACAGAAA 
GTGAAGAAAT CTGTAAAAGC AATATATAAT TCCGGTCAAG ATCATGTACA AAATGAAGAA 
AACTATGCAC AAGTTCTGGA TAAGTTTGGA AGTAATTTTT TAAGCAGAGA CAACCCTGAC 
CTTGGCACCG CTTTTGTCAA GTTTTCTACA CTCACAAAGG AACTGTCCAC GCTGCTGAAA 
AATCTGCTCC AGGGTCTGAG CCACAATGTG ATCTTCACCT TGGATTCCTT GTTAAAAGGA 
GACTTGAAGG GGGTCAAAGG AGATCTCAAG AAGCCATTTG ACAAAGCCTG GAAAGATTAT 
GAGACGAAGT TTACAAAAAT TGAGAAGGAG AAAAGAGAGC ACGCAAAACA GCACGGGATG 
ATCCGAACAG AGATAACAGG CGCTGAGATT GCAGAAGAAA TGGAAAAGGA ACGGCGGCTT 
TTTCAGCTCC AGATGTGTGA ATATCTCATT AAAGTAAATG AAATCAAGAC CAAAAAGGGT 
GTGGACCTGC TGCAGAACCT TATAAAGTAT TACCATGCAC AGTGCAATTT CTTTCAGGAT 
GGCTTGAAAA CGGCTGATAA ACTGAAACAG TACATAGAAA AGCTGGCTGC TGACTTATAT 
AATATAAAGC AAACCCAAGA TGAAGAAAAA AAACAGCTAA CTGCACTCCG AGATCTAATA 
AAATCCTCTC TGCAACTTGA TCCGAAAGAA GTAGGTGGTT TATATGTTGC CAGCAGGGCT 
AACAGTTCTA GAAGAGACTC TCAGAGCCGG CAAGGTGGGT ACAGCATGCA TCAGCTCCAG 
GGCAACAAGG AGTACGGCAG TGAAAAGAAG GGCTTCCTGC TGAAGAAGAG TGATGGGATC 
CGGAAAGTGT GGCAGAGACG GAAGTGTGCG GTCAAGAACG GGATCCTGAC CATCTCCCAC 
GCAACTTCCA ACAGGCAGCC TGCTAAGTTA AACCTCCTCA CCTGCCAAGT GAAACCGAAT 
GCTGAGGACA AGAAGTCTTT TGACCTGATA TCACATAATC GAACATATCA CTTTCAAGCA 
GAAGATGAGC AGGATTATAT AGCGTGGATA TCAGTACTGA CGAATAGCAA AGAAGAGGCC 
CTAACCATGG CCTTCCGTGG TGAGCAGAGC ACAGGGGAGA ATAGCCTGGA GGACCTTACC 
AAAGCCATCA TCGAGGATGT ACAGCGGCTC CCTGGGAATG ACATCTGCTG TGACTGCGGC 
TCATCAGAAC CCACGTGGCT TTCAACCAAC TTGGGTATTT TGACCTGTAT AGAATGTTCC 
GGAATCCATA GGGAAATGGG GGTTCATATT TCTCGCATTC AGTCTTTGGA ACTAGACAAA 
TTAGGAACTT CCGAACTCTT GCTGGCCAAG AATGTAGGAA ACAATAGTTT TAATGATATT 
ATGGAAGCAA ATTTACCCAG CCCTTCACCA AAGCCCACCC CTTCAAGTGA TATGACCGTG 
CGTAAAGAGT ACATCACTGC AAAGTATGTC GACCACCGCT TCTCCAGGAA GACCTGTGCC 
TCTTCCTCAG CAAAGCTGAA CGAATTGCTT GAGGCCATCA AGTCCAGGGA CTTACTTGCA 
TTAATCCAAG TCTATGCAGA GGGGGTGGAG TTAATGGAGC CACTGCTGGA ACCTGGGCAG 
GAGCTCGGGG AGACAGCCCT TCATCTTGCC GTCAGGACCG CAGACCAGAC ATCTCTCCAT 
CTGGTTGATT TCCTTGTACA AAACTGTGGG AACCTGGATA AGCAAACGTC TGTGGGGAAT 
ACAGTTCTGC ACTATTGCAG CATGTACGGC AAGCCGGAGT GTCTGAAGCT GCTGCTTAGG 
AGCAAGCCCA CCGTGGACAT CGTTAACCAG AATGGAGAAA CTGCCTTGGA TATAGCAAAG 
AGACTCAAAG CTACCCAGTG TGAAGATCTG CTTTCCCAGG CTAAATCTGG AAAGTTCAAC 
CCTCATGTCC ACGTAGAGTA TGAGTGGAAT CTTCGACAGG ACGAGATGGA TGAGAGCGAT 
GACGATCTGG ATGACAAGCC AAGCCCGATC AAAAAGGAAC GCTCGCCCAG ACCACAGAGC 
TTCTGCCACT CCTCCAGCAT CTCGCCACAG GACAAGCTGG CACTGCCAGG GTTCAGCACT 
CCACGGGACA AGCAGCGGCT CTCCTACGGA GCCTTCACCA ACCAGATCTT CGCCTCTACG 
AGCACAGATT TGCCCACATC ACCCACCAGT GAGGCTCCCC CTTTGCCACC TCGGAACGCC 
GGGAAAGGTC CAACTGGCCC ACCTTCAACA CTCCCTCTAG GCACCCAGAC CTCTAGTGGC 
AGCTCCACCC TATCCAAGAA GAGGCCTCCT CCCCCACCAC CAGGACACAA GAGAACCCTG 
TCTGACCCTC CCAGCCCACT ACCTCACGGG CCCCCAAACA AAGGCGCAAT TCCTTGGGGT 
AATGATGTGG GCCCATTATC TTCAAGTAAG ACGGCCAACA AGTTTGAGGG GCTGTCTCAG 
CAAGCAAGCA CCAGTTCTGC TAAGACTGCC CTTGGCCCGA GAGTGCTTCC TAAACTACCT 
CAGAAAGTGG CACTAAGGAA GACGGAGACC AGCCATCATC TCTCCCTCGA CAGAACCAAC 
ATCCCACCTG AGACTTTTCA GAAATCATCA CAGTTGACAG AGTTACCCCA AAAGCCACCA 
CTTGGAGAGC TGCCCCCGAA GCCTGTGGAA CTGGCCCCCA AGCCCCAAGT TGGAGAGCTG 
CCACCTAAGC CTGGAGAGCT ACCCCCTAAG CCCCAATTAG GTGACCTGCC CCCCAAGCCA 
CAGCTCTCAG ACTTACCTCC CAAGCCACAG ATGAAGGACC TGCCCCCTAA GCCGCAGCTG 
GGGGATCTGC TGGCAAAGTC CCAGGCTGGC GATGTCTCAG CCAAGGTGCA GCCACCCTCA 
GAGGTCACAC AGAGGTCACA CACCGGGGAT CTGTCTCCAA ATGTACAGTC CAGAGATGCC 
ATCCAGAAGC AAGCATCTGA AGACTCCAAC GACCTCACAC CCACGCTGCC AGAGACACCC 
GTACCACTGC CGAGAAAAAT CAATACGGGG AAAAATAAAG TGAGGCGGGT GAAGACCATT 
TATGACTGCC AGGCAGATAA TGACGACGAA CTCACATTTA TTGAGGGGGA AGTGATCATT 
GTCACCGGGG AAGAGGACCA GGAGTGGTGG ATCGGGCATA TCGAAGGACA GCCTGAAAGG 
AAGGGTGTCT TTCCAGTGTC CTTTGTCCAC ATCCTGTCTG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asap1 ( XM_006520397.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006520460.1 Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OMu50918
Clone ID Related Accession (Same CDS sequence)	XM_006520397.2
Accession Version	XM_006520397.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006520397.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAGAT GCATGCTTTG CATTCCTTTT GAATTCCCTT TGAGTCCCCA GGTCAGGCTC 
CAACACCATG TAAGGGATGG GCTTTCGACT GTGCTCATCC TGCCTTTCAT GTCTGCTCTG 
CTTCCCCAGC GTGAAGCTAG TGCCGAAGAG CTCCGCCAGC TTTATGAGTG GAGCAGGAAC 
CCACACTCAT TAAACACAAC AGGATGCCGG ACCAGATCTC CGTGTCCGAG TTCATCGCCG 
AGACCACCGA GGACTACAAC TCGCCCACCA CGTCCAGCTT CACCACGCGG CTGCACAACT 
GCAGGAACAC CGTCACGCTG CTGGAGGAGC GTGGTTGACG GTGGCAGCCG AGCAGTGGTG 
CAGATGACGC CAGCTGTGTT GCACTTGGGT GAGCCATACA CAAGTTCATT TGAGGCTCTA 
GACCAAGATA GAACAGCCCT ACAGAAAGTG AAGAAATCTG TAAAAGCAAT ATATAATTCC 
GGTCAAGATC ATGTACAAAA TGAAGAAAAC TATGCACAAG TTCTGGATAA GTTTGGAAGT 
AATTTTTTAA GCAGAGACAA CCCTGACCTT GGCACCGCTT TTGTCAAGTT TTCTACACTC 
ACAAAGGAAC TGTCCACGCT GCTGAAAAAT CTGCTCCAGG GTCTGAGCCA CAATGTGATC 
TTCACCTTGG ATTCCTTGTT AAAAGGAGAC TTGAAGGGGG TCAAAGGAGA TCTCAAGAAG 
CCATTTGACA AAGCCTGGAA AGATTATGAG ACGAAGTTTA CAAAAATTGA GAAGGAGAAA 
AGAGAGCACG CAAAACAGCA CGGGATGATC CGAACAGAGA TAACAGGCGC TGAGATTGCA 
GAAGAAATGG AAAAGGAACG GCGGCTTTTT CAGCTCCAGA TGTGTGAATA TCTCATTAAA 
GTAAATGAAA TCAAGACCAA AAAGGGTGTG GACCTGCTGC AGAACCTTAT AAAGTATTAC 
CATGCACAGT GCAATTTCTT TCAGGATGGC TTGAAAACGG CTGATAAACT GAAACAGTAC 
ATAGAAAAGC TGGCTGCTGA CTTATATAAT ATAAAGCAAA CCCAAGATGA AGAAAAAAAA 
CAGCTAACTG CACTCCGAGA TCTAATAAAA TCCTCTCTGC AACTTGATCC GAAAGAAGTA 
GGTGGTTTAT ATGTTGCCAG CAGGGCTAAC AGTTCTAGAA GAGACTCTCA GAGCCGGCAA 
GGTGGGTACA GCATGCATCA GCTCCAGGGC AACAAGGAGT ACGGCAGTGA AAAGAAGGGC 
TTCCTGCTGA AGAAGAGTGA TGGGATCCGG AAAGTGTGGC AGAGACGGAA GTGTGCGGTC 
AAGAACGGGA TCCTGACCAT CTCCCACGCA ACTTCCAACA GGCAGCCTGC TAAGTTAAAC 
CTCCTCACCT GCCAAGTGAA ACCGAATGCT GAGGACAAGA AGTCTTTTGA CCTGATATCA 
CATAATCGAA CATATCACTT TCAAGCAGAA GATGAGCAGG ATTATATAGC GTGGATATCA 
GTACTGACGA ATAGCAAAGA AGAGGCCCTA ACCATGGCCT TCCGTGGTGA GCAGAGCACA 
GGGGAGAATA GCCTGGAGGA CCTTACCAAA GCCATCATCG AGGATGTACA GCGGCTCCCT 
GGGAATGACA TCTGCTGTGA CTGCGGCTCA TCAGAACCCA CGTGGCTTTC AACCAACTTG 
GGTATTTTGA CCTGTATAGA ATGTTCCGGA ATCCATAGGG AAATGGGGGT TCATATTTCT 
CGCATTCAGT CTTTGGAACT AGACAAATTA GGAACTTCCG AACTCTTGCT GGCCAAGAAT 
GTAGGAAACA ATAGTTTTAA TGATATTATG GAAGCAAATT TACCCAGCCC TTCACCAAAG 
CCCACCCCTT CAAGTGATAT GACCGTGCGT AAAGAGTACA TCACTGCAAA GTATGTCGAC 
CACCGCTTCT CCAGGAAGAC CTGTGCCTCT TCCTCAGCAA AGCTGAACGA ATTGCTTGAG 
GCCATCAAGT CCAGGGACTT ACTTGCATTA ATCCAAGTCT ATGCAGAGGG GGTGGAGTTA 
ATGGAGCCAC TGCTGGAACC TGGGCAGGAG CTCGGGGAGA CAGCCCTTCA TCTTGCCGTC 
AGGACCGCAG ACCAGACATC TCTCCATCTG GTTGATTTCC TTGTACAAAA CTGTGGGAAC 
CTGGATAAGC AAACGTCTGT GGGGAATACA GTTCTGCACT ATTGCAGCAT GTACGGCAAG 
CCGGAGTGTC TGAAGCTGCT GCTTAGGAGC AAGCCCACCG TGGACATCGT TAACCAGAAT 
GGAGAAACTG CCTTGGATAT AGCAAAGAGA CTCAAAGCTA CCCAGTGTGA AGATCTGCTT 
TCCCAGGCTA AATCTGGAAA GTTCAACCCT CATGTCCACG TAGAGTATGA GTGGAATCTT 
CGACAGGACG AGATGGATGA GAGCGATGAC GATCTGGATG ACAAGCCAAG CCCGATCAAA 
AAGGAACGCT CGCCCAGACC ACAGAGCTTC TGCCACTCCT CCAGCATCTC GCCACAGGAC 
AAGCTGGCAC TGCCAGGGTT CAGCACTCCA CGGGACAAGC AGCGGCTCTC CTACGGAGCC 
TTCACCAACC AGATCTTCGC CTCTACGAGC ACAGATTTGC CCACATCACC CACCAGTGAG 
GCTCCCCCTT TGCCACCTCG GAACGCCGGG AAAGGTAATG ATGTGGGCCC ATTATCTTCA 
AGTAAGACGG CCAACAAGTT TGAGGGGCTG TCTCAGCAAG CAAGCACCAG TTCTGCTAAG 
ACTGCCCTTG GCCCGAGAGT GCTTCCTAAA CTACCTCAGA AAGTGGCACT AAGGAAGACG 
GAGACCAGCC ATCATCTCTC CCTCGACAGA ACCAACATCC CACCTGAGAC TTTTCAGAAA 
TCATCACAGT TGACAGAGTT ACCCCAAAAG CCACCACTTG GAGAGCTGCC CCCGAAGCCT 
GTGGAACTGG CCCCCAAGCC CCAAGTTGGA GAGCTGCCAC CTAAGCCTGG AGAGCTACCC 
CCTAAGCCCC AATTAGGTGA CCTGCCCCCC AAGCCACAGC TCTCAGACTT ACCTCCCAAG 
CCACAGATGA AGGACCTGCC CCCTAAGCCG CAGCTGGGGG ATCTGCTGGC AAAGTCCCAG 
GCTGGCGATG TCTCAGCCAA GGTGCAGCCA CCCTCAGAGG TCACACAGAG GTCACACACC 
GGGGATCTGT CTCCAAATGT ACAGTCCAGA GATGCCATCC AGAAGCAAGC ATCTGAAGAC 
TCCAACGACC TCACACCCAC GCTGCCAGAG ACACCCGTAC CACTGCCGAG AAAAATCAAT 
ACGGGGAAAA ATAAAGTGAG GCGGGTGAAG ACCATTTATG ACTGCCAGGC AGATAATGAC 
GACGAACTCA CATTTATTGA GGGGGAAGTG ATCATTGTCA CCGGGGAAGA GGACCAGGAG 
TGGTGGATCG GGCATATCGA AGGACAGCCT GAAAGGAAGG GTGTCTTTCC AGTGTCCTTT 
GTCCACATCC TGTCTGACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006520460.1
CDS	16..3516
Translation	MSRCMLCIPFEFPLSPQVRLQHHVRDGLSTVLILPFMSALLPQREASAEELRQLYEWSRNPHSLNTTGCRTRSPCPSSSPRPPRTTTRPPRPASPRGCTTAGTPSRCWRSVVDGGSRAVVQMTPAVLHLGEPYTSSFEALDQDRTALQKVKKSVKAIYNSGQDHVQNEENYAQVLDKFGSNFLSRDNPDLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGVKGDLKKPFDKAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDPKEVGGLYVASRANSSRRDSQSRQGGYSMHQLQGNKEYGSEKKGFLLKKSDGIRKVWQRRKCAVKNGILTISHATSNRQPAKLNLLTCQVKPNAEDKKSFDLISHNRTYHFQAEDEQDYIAWISVLTNSKEEALTMAFRGEQSTGENSLEDLTKAIIEDVQRLPGNDICCDCGSSEPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDKLGTSELLLAKNVGNNSFNDIMEANLPSPSPKPTPSSDMTVRKEYITAKYVDHRFSRKTCASSSAKLNELLEAIKSRDLLALIQVYAEGVELMEPLLEPGQELGETALHLAVRTADQTSLHLVDFLVQNCGNLDKQTSVGNTVLHYCSMYGKPECLKLLLRSKPTVDIVNQNGETALDIAKRLKATQCEDLLSQAKSGKFNPHVHVEYEWNLRQDEMDESDDDLDDKPSPIKKERSPRPQSFCHSSSISPQDKLALPGFSTPRDKQRLSYGAFTNQIFASTSTDLPTSPTSEAPPLPPRNAGKGNDVGPLSSSKTANKFEGLSQQASTSSAKTALGPRVLPKLPQKVALRKTETSHHLSLDRTNIPPETFQKSSQLTELPQKPPLGELPPKPVELAPKPQVGELPPKPGELPPKPQLGDLPPKPQLSDLPPKPQMKDLPPKPQLGDLLAKSQAGDVSAKVQPPSEVTQRSHTGDLSPNVQSRDAIQKQASEDSNDLTPTLPETPVPLPRKINTGKNKVRRVKTIYDCQADNDDELTFIEGEVIIVTGEEDQEWWIGHIEGQPERKGVFPVSFVHILSD

Target ORF information:

RefSeq Version	XM_006520397.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006520397.2

ORF Insert Sequence:

ATGTCGAGAT GCATGCTTTG CATTCCTTTT GAATTCCCTT TGAGTCCCCA GGTCAGGCTC 
CAACACCATG TAAGGGATGG GCTTTCGACT GTGCTCATCC TGCCTTTCAT GTCTGCTCTG 
CTTCCCCAGC GTGAAGCTAG TGCCGAAGAG CTCCGCCAGC TTTATGAGTG GAGCAGGAAC 
CCACACTCAT TAAACACAAC AGGATGCCGG ACCAGATCTC CGTGTCCGAG TTCATCGCCG 
AGACCACCGA GGACTACAAC TCGCCCACCA CGTCCAGCTT CACCACGCGG CTGCACAACT 
GCAGGAACAC CGTCACGCTG CTGGAGGAGC GTGGTTGACG GTGGCAGCCG AGCAGTGGTG 
CAGATGACGC CAGCTGTGTT GCACTTGGGT GAGCCATACA CAAGTTCATT TGAGGCTCTA 
GACCAAGATA GAACAGCCCT ACAGAAAGTG AAGAAATCTG TAAAAGCAAT ATATAATTCC 
GGTCAAGATC ATGTACAAAA TGAAGAAAAC TATGCACAAG TTCTGGATAA GTTTGGAAGT 
AATTTTTTAA GCAGAGACAA CCCTGACCTT GGCACCGCTT TTGTCAAGTT TTCTACACTC 
ACAAAGGAAC TGTCCACGCT GCTGAAAAAT CTGCTCCAGG GTCTGAGCCA CAATGTGATC 
TTCACCTTGG ATTCCTTGTT AAAAGGAGAC TTGAAGGGGG TCAAAGGAGA TCTCAAGAAG 
CCATTTGACA AAGCCTGGAA AGATTATGAG ACGAAGTTTA CAAAAATTGA GAAGGAGAAA 
AGAGAGCACG CAAAACAGCA CGGGATGATC CGAACAGAGA TAACAGGCGC TGAGATTGCA 
GAAGAAATGG AAAAGGAACG GCGGCTTTTT CAGCTCCAGA TGTGTGAATA TCTCATTAAA 
GTAAATGAAA TCAAGACCAA AAAGGGTGTG GACCTGCTGC AGAACCTTAT AAAGTATTAC 
CATGCACAGT GCAATTTCTT TCAGGATGGC TTGAAAACGG CTGATAAACT GAAACAGTAC 
ATAGAAAAGC TGGCTGCTGA CTTATATAAT ATAAAGCAAA CCCAAGATGA AGAAAAAAAA 
CAGCTAACTG CACTCCGAGA TCTAATAAAA TCCTCTCTGC AACTTGATCC GAAAGAAGTA 
GGTGGTTTAT ATGTTGCCAG CAGGGCTAAC AGTTCTAGAA GAGACTCTCA GAGCCGGCAA 
GGTGGGTACA GCATGCATCA GCTCCAGGGC AACAAGGAGT ACGGCAGTGA AAAGAAGGGC 
TTCCTGCTGA AGAAGAGTGA TGGGATCCGG AAAGTGTGGC AGAGACGGAA GTGTGCGGTC 
AAGAACGGGA TCCTGACCAT CTCCCACGCA ACTTCCAACA GGCAGCCTGC TAAGTTAAAC 
CTCCTCACCT GCCAAGTGAA ACCGAATGCT GAGGACAAGA AGTCTTTTGA CCTGATATCA 
CATAATCGAA CATATCACTT TCAAGCAGAA GATGAGCAGG ATTATATAGC GTGGATATCA 
GTACTGACGA ATAGCAAAGA AGAGGCCCTA ACCATGGCCT TCCGTGGTGA GCAGAGCACA 
GGGGAGAATA GCCTGGAGGA CCTTACCAAA GCCATCATCG AGGATGTACA GCGGCTCCCT 
GGGAATGACA TCTGCTGTGA CTGCGGCTCA TCAGAACCCA CGTGGCTTTC AACCAACTTG 
GGTATTTTGA CCTGTATAGA ATGTTCCGGA ATCCATAGGG AAATGGGGGT TCATATTTCT 
CGCATTCAGT CTTTGGAACT AGACAAATTA GGAACTTCCG AACTCTTGCT GGCCAAGAAT 
GTAGGAAACA ATAGTTTTAA TGATATTATG GAAGCAAATT TACCCAGCCC TTCACCAAAG 
CCCACCCCTT CAAGTGATAT GACCGTGCGT AAAGAGTACA TCACTGCAAA GTATGTCGAC 
CACCGCTTCT CCAGGAAGAC CTGTGCCTCT TCCTCAGCAA AGCTGAACGA ATTGCTTGAG 
GCCATCAAGT CCAGGGACTT ACTTGCATTA ATCCAAGTCT ATGCAGAGGG GGTGGAGTTA 
ATGGAGCCAC TGCTGGAACC TGGGCAGGAG CTCGGGGAGA CAGCCCTTCA TCTTGCCGTC 
AGGACCGCAG ACCAGACATC TCTCCATCTG GTTGATTTCC TTGTACAAAA CTGTGGGAAC 
CTGGATAAGC AAACGTCTGT GGGGAATACA GTTCTGCACT ATTGCAGCAT GTACGGCAAG 
CCGGAGTGTC TGAAGCTGCT GCTTAGGAGC AAGCCCACCG TGGACATCGT TAACCAGAAT 
GGAGAAACTG CCTTGGATAT AGCAAAGAGA CTCAAAGCTA CCCAGTGTGA AGATCTGCTT 
TCCCAGGCTA AATCTGGAAA GTTCAACCCT CATGTCCACG TAGAGTATGA GTGGAATCTT 
CGACAGGACG AGATGGATGA GAGCGATGAC GATCTGGATG ACAAGCCAAG CCCGATCAAA 
AAGGAACGCT CGCCCAGACC ACAGAGCTTC TGCCACTCCT CCAGCATCTC GCCACAGGAC 
AAGCTGGCAC TGCCAGGGTT CAGCACTCCA CGGGACAAGC AGCGGCTCTC CTACGGAGCC 
TTCACCAACC AGATCTTCGC CTCTACGAGC ACAGATTTGC CCACATCACC CACCAGTGAG 
GCTCCCCCTT TGCCACCTCG GAACGCCGGG AAAGGTAATG ATGTGGGCCC ATTATCTTCA 
AGTAAGACGG CCAACAAGTT TGAGGGGCTG TCTCAGCAAG CAAGCACCAG TTCTGCTAAG 
ACTGCCCTTG GCCCGAGAGT GCTTCCTAAA CTACCTCAGA AAGTGGCACT AAGGAAGACG 
GAGACCAGCC ATCATCTCTC CCTCGACAGA ACCAACATCC CACCTGAGAC TTTTCAGAAA 
TCATCACAGT TGACAGAGTT ACCCCAAAAG CCACCACTTG GAGAGCTGCC CCCGAAGCCT 
GTGGAACTGG CCCCCAAGCC CCAAGTTGGA GAGCTGCCAC CTAAGCCTGG AGAGCTACCC 
CCTAAGCCCC AATTAGGTGA CCTGCCCCCC AAGCCACAGC TCTCAGACTT ACCTCCCAAG 
CCACAGATGA AGGACCTGCC CCCTAAGCCG CAGCTGGGGG ATCTGCTGGC AAAGTCCCAG 
GCTGGCGATG TCTCAGCCAA GGTGCAGCCA CCCTCAGAGG TCACACAGAG GTCACACACC 
GGGGATCTGT CTCCAAATGT ACAGTCCAGA GATGCCATCC AGAAGCAAGC ATCTGAAGAC 
TCCAACGACC TCACACCCAC GCTGCCAGAG ACACCCGTAC CACTGCCGAG AAAAATCAAT 
ACGGGGAAAA ATAAAGTGAG GCGGGTGAAG ACCATTTATG ACTGCCAGGC AGATAATGAC 
GACGAACTCA CATTTATTGA GGGGGAAGTG ATCATTGTCA CCGGGGAAGA GGACCAGGAG 
TGGTGGATCG GGCATATCGA AGGACAGCCT GAAAGGAAGG GTGTCTTTCC AGTGTCCTTT 
GTCCACATCC TGTCTGACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asap1 ( XM_006520398.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006520461.1 Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X7, mRNA.

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CloneID	OMu50919
Clone ID Related Accession (Same CDS sequence)	XM_006520398.3 , XM_006520398.4
Accession Version	XM_006520398.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006520398.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCAG GTCTGAGTGG GCCACAGGGC TTGCCTTCCA AAGCAGGCTT TAATGGAGGC 
CAGTCATACG CTTTCAAGGG CTCCTGGAGG CCAATCCCAA GGACTAGGAT GCCGGACCAG 
ATCTCCGTGT CCGAGTTCAT CGCCGAGACC ACCGAGGACT ACAACTCGCC CACCACGTCC 
AGCTTCACCA CGCGGCTGCA CAACTGCAGG AACACCGTCA CGCTGCTGGA GGAGGCTCTA 
GACCAAGATA GAACAGCCCT ACAGAAAGTG AAGAAATCTG TAAAAGCAAT ATATAATTCC 
GGTCAAGATC ATGTACAAAA TGAAGAAAAC TATGCACAAG TTCTGGATAA GTTTGGAAGT 
AATTTTTTAA GCAGAGACAA CCCTGACCTT GGCACCGCTT TTGTCAAGTT TTCTACACTC 
ACAAAGGAAC TGTCCACGCT GCTGAAAAAT CTGCTCCAGG GTCTGAGCCA CAATGTGATC 
TTCACCTTGG ATTCCTTGTT AAAAGGAGAC TTGAAGGGGG TCAAAGGAGA TCTCAAGAAG 
CCATTTGACA AAGCCTGGAA AGATTATGAG ACGAAGTTTA CAAAAATTGA GAAGGAGAAA 
AGAGAGCACG CAAAACAGCA CGGGATGATC CGAACAGAGA TAACAGGCGC TGAGATTGCA 
GAAGAAATGG AAAAGGAACG GCGGCTTTTT CAGCTCCAGA TGTGTGAATA TCTCATTAAA 
GTAAATGAAA TCAAGACCAA AAAGGGTGTG GACCTGCTGC AGAACCTTAT AAAGTATTAC 
CATGCACAGT GCAATTTCTT TCAGGATGGC TTGAAAACGG CTGATAAACT GAAACAGTAC 
ATAGAAAAGC TGGCTGCTGA CTTATATAAT ATAAAGCAAA CCCAAGATGA AGAAAAAAAA 
CAGCTAACTG CACTCCGAGA TCTAATAAAA TCCTCTCTGC AACTTGATCC GAAAGAAGTA 
GGTGGTTTAT ATGTTGCCAG CAGGGCTAAC AGTTCTAGAA GAGACTCTCA GAGCCGGCAA 
GGTGGGTACA GCATGCATCA GCTCCAGGGC AACAAGGAGT ACGGCAGTGA AAAGAAGGGC 
TTCCTGCTGA AGAAGAGTGA TGGGATCCGG AAAGTGTGGC AGAGACGGAA GTGTGCGGTC 
AAGAACGGGA TCCTGACCAT CTCCCACGCA ACTTCCAACA GGCAGCCTGC TAAGTTAAAC 
CTCCTCACCT GCCAAGTGAA ACCGAATGCT GAGGACAAGA AGTCTTTTGA CCTGATATCA 
CATAATCGAA CATATCACTT TCAAGCAGAA GATGAGCAGG ATTATATAGC GTGGATATCA 
GTACTGACGA ATAGCAAAGA AGAGGCCCTA ACCATGGCCT TCCGTGGTGA GCAGAGCACA 
GGGGAGAATA GCCTGGAGGA CCTTACCAAA GCCATCATCG AGGATGTACA GCGGCTCCCT 
GGGAATGACA TCTGCTGTGA CTGCGGCTCA TCAGAACCCA CGTGGCTTTC AACCAACTTG 
GGTATTTTGA CCTGTATAGA ATGTTCCGGA ATCCATAGGG AAATGGGGGT TCATATTTCT 
CGCATTCAGT CTTTGGAACT AGACAAATTA GGAACTTCCG AACTCTTGCT GGCCAAGAAT 
GTAGGAAACA ATAGTTTTAA TGATATTATG GAAGCAAATT TACCCAGCCC TTCACCAAAG 
CCCACCCCTT CAAGTGATAT GACCGTGCGT AAAGAGTACA TCACTGCAAA GTATGTCGAC 
CACCGCTTCT CCAGGAAGAC CTGTGCCTCT TCCTCAGCAA AGCTGAACGA ATTGCTTGAG 
GCCATCAAGT CCAGGGACTT ACTTGCATTA ATCCAAGTCT ATGCAGAGGG GGTGGAGTTA 
ATGGAGCCAC TGCTGGAACC TGGGCAGGAG CTCGGGGAGA CAGCCCTTCA TCTTGCCGTC 
AGGACCGCAG ACCAGACATC TCTCCATCTG GTTGATTTCC TTGTACAAAA CTGTGGGAAC 
CTGGATAAGC AAACGTCTGT GGGGAATACA GTTCTGCACT ATTGCAGCAT GTACGGCAAG 
CCGGAGTGTC TGAAGCTGCT GCTTAGGAGC AAGCCCACCG TGGACATCGT TAACCAGAAT 
GGAGAAACTG CCTTGGATAT AGCAAAGAGA CTCAAAGCTA CCCAGTGTGA AGATCTGCTT 
TCCCAGGCTA AATCTGGAAA GTTCAACCCT CATGTCCACG TAGAGTATGA GTGGAATCTT 
CGACAGGACG AGATGGATGA GAGCGATGAC GATCTGGATG ACAAGCCAAG CCCGATCAAA 
AAGGAACGCT CGCCCAGACC ACAGAGCTTC TGCCACTCCT CCAGCATCTC GCCACAGGAC 
AAGCTGGCAC TGCCAGGGTT CAGCACTCCA CGGGACAAGC AGCGGCTCTC CTACGGAGCC 
TTCACCAACC AGATCTTCGC CTCTACGAGC ACAGATTTGC CCACATCACC CACCAGTGAG 
GCTCCCCCTT TGCCACCTCG GAACGCCGGG AAAGGTCCAA CTGGCCCACC TTCAACACTC 
CCTCTAGGCA CCCAGACCTC TAGTGGCAGC TCCACCCTAT CCAAGAAGAG GCCTCCTCCC 
CCACCACCAG GACACAAGAG AACCCTGTCT GACCCTCCCA GCCCACTACC TCACGGGCCC 
CCAAACAAAG GCGCAATTCC TTGGGGTAAT GATGTGGGCC CATTATCTTC AAGTAAGACG 
GCCAACAAGT TTGAGGGGCT GTCTCAGCAA GCAAGCACCA GTTCTGCTAA GACTGCCCTT 
GGCCCGAGAG TGCTTCCTAA ACTACCTCAG AAAGTGGCAC TAAGGAAGAC GGAGACCAGC 
CATCATCTCT CCCTCGACAG AACCAACATC CCACCTGAGA CTTTTCAGAA ATCATCACAG 
TTGACAGAGT TACCCCAAAA GCCACCACTT GGAGAGCTGC CCCCGAAGCC TGTGGAACTG 
GCCCCCAAGC CCCAAGTTGG AGAGCTGCCA CCTAAGCCTG GAGAGCTACC CCCTAAGCCC 
CAATTAGGTG ACCTGCCCCC CAAGCCACAG CTCTCAGACT TACCTCCCAA GCCACAGATG 
AAGGACCTGC CCCCTAAGCC GCAGCTGGGG GATCTGCTGG CAAAGTCCCA GGCTGGCGAT 
GTCTCAGCCA AGGTGCAGCC ACCCTCAGAG GTCACACAGA GGTCACACAC CGGGGATCTG 
TCTCCAAATG TACAGTCCAG AGATGCCATC CAGAAGCAAG CATCTGAAGA CTCCAACGAC 
CTCACACCCA CGCTGCCAGA GACACCCGTA CCACTGCCGA GAAAAATCAA TACGGGGAAA 
AATAAAGTGA GGCGGGTGAA GACCATTTAT GACTGCCAGG CAGATAATGA CGACGAACTC 
ACATTTATTG AGGGGGAAGT GATCATTGTC ACCGGGGAAG AGGACCAGGA GTGGTGGATC 
GGGCATATCG AAGGACAGCC TGAAAGGAAG GGTGTCTTTC CAGTGTCCTT TGTCCACATC 
CTGTCTGACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006520461.1
CDS	453..3944
Translation	MQAGLSGPQGLPSKAGFNGGQSYAFKGSWRPIPRTRMPDQISVSEFIAETTEDYNSPTTSSFTTRLHNCRNTVTLLEEALDQDRTALQKVKKSVKAIYNSGQDHVQNEENYAQVLDKFGSNFLSRDNPDLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGVKGDLKKPFDKAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDPKEVGGLYVASRANSSRRDSQSRQGGYSMHQLQGNKEYGSEKKGFLLKKSDGIRKVWQRRKCAVKNGILTISHATSNRQPAKLNLLTCQVKPNAEDKKSFDLISHNRTYHFQAEDEQDYIAWISVLTNSKEEALTMAFRGEQSTGENSLEDLTKAIIEDVQRLPGNDICCDCGSSEPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDKLGTSELLLAKNVGNNSFNDIMEANLPSPSPKPTPSSDMTVRKEYITAKYVDHRFSRKTCASSSAKLNELLEAIKSRDLLALIQVYAEGVELMEPLLEPGQELGETALHLAVRTADQTSLHLVDFLVQNCGNLDKQTSVGNTVLHYCSMYGKPECLKLLLRSKPTVDIVNQNGETALDIAKRLKATQCEDLLSQAKSGKFNPHVHVEYEWNLRQDEMDESDDDLDDKPSPIKKERSPRPQSFCHSSSISPQDKLALPGFSTPRDKQRLSYGAFTNQIFASTSTDLPTSPTSEAPPLPPRNAGKGPTGPPSTLPLGTQTSSGSSTLSKKRPPPPPPGHKRTLSDPPSPLPHGPPNKGAIPWGNDVGPLSSSKTANKFEGLSQQASTSSAKTALGPRVLPKLPQKVALRKTETSHHLSLDRTNIPPETFQKSSQLTELPQKPPLGELPPKPVELAPKPQVGELPPKPGELPPKPQLGDLPPKPQLSDLPPKPQMKDLPPKPQLGDLLAKSQAGDVSAKVQPPSEVTQRSHTGDLSPNVQSRDAIQKQASEDSNDLTPTLPETPVPLPRKINTGKNKVRRVKTIYDCQADNDDELTFIEGEVIIVTGEEDQEWWIGHIEGQPERKGVFPVSFVHILSD

Target ORF information:

RefSeq Version	XM_006520398.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006520398.3

ORF Insert Sequence:

ATGCAGGCAG GTCTGAGTGG GCCACAGGGC TTGCCTTCCA AAGCAGGCTT TAATGGAGGC 
CAGTCATACG CTTTCAAGGG CTCCTGGAGG CCAATCCCAA GGACTAGGAT GCCGGACCAG 
ATCTCCGTGT CCGAGTTCAT CGCCGAGACC ACCGAGGACT ACAACTCGCC CACCACGTCC 
AGCTTCACCA CGCGGCTGCA CAACTGCAGG AACACCGTCA CGCTGCTGGA GGAGGCTCTA 
GACCAAGATA GAACAGCCCT ACAGAAAGTG AAGAAATCTG TAAAAGCAAT ATATAATTCC 
GGTCAAGATC ATGTACAAAA TGAAGAAAAC TATGCACAAG TTCTGGATAA GTTTGGAAGT 
AATTTTTTAA GCAGAGACAA CCCTGACCTT GGCACCGCTT TTGTCAAGTT TTCTACACTC 
ACAAAGGAAC TGTCCACGCT GCTGAAAAAT CTGCTCCAGG GTCTGAGCCA CAATGTGATC 
TTCACCTTGG ATTCCTTGTT AAAAGGAGAC TTGAAGGGGG TCAAAGGAGA TCTCAAGAAG 
CCATTTGACA AAGCCTGGAA AGATTATGAG ACGAAGTTTA CAAAAATTGA GAAGGAGAAA 
AGAGAGCACG CAAAACAGCA CGGGATGATC CGAACAGAGA TAACAGGCGC TGAGATTGCA 
GAAGAAATGG AAAAGGAACG GCGGCTTTTT CAGCTCCAGA TGTGTGAATA TCTCATTAAA 
GTAAATGAAA TCAAGACCAA AAAGGGTGTG GACCTGCTGC AGAACCTTAT AAAGTATTAC 
CATGCACAGT GCAATTTCTT TCAGGATGGC TTGAAAACGG CTGATAAACT GAAACAGTAC 
ATAGAAAAGC TGGCTGCTGA CTTATATAAT ATAAAGCAAA CCCAAGATGA AGAAAAAAAA 
CAGCTAACTG CACTCCGAGA TCTAATAAAA TCCTCTCTGC AACTTGATCC GAAAGAAGTA 
GGTGGTTTAT ATGTTGCCAG CAGGGCTAAC AGTTCTAGAA GAGACTCTCA GAGCCGGCAA 
GGTGGGTACA GCATGCATCA GCTCCAGGGC AACAAGGAGT ACGGCAGTGA AAAGAAGGGC 
TTCCTGCTGA AGAAGAGTGA TGGGATCCGG AAAGTGTGGC AGAGACGGAA GTGTGCGGTC 
AAGAACGGGA TCCTGACCAT CTCCCACGCA ACTTCCAACA GGCAGCCTGC TAAGTTAAAC 
CTCCTCACCT GCCAAGTGAA ACCGAATGCT GAGGACAAGA AGTCTTTTGA CCTGATATCA 
CATAATCGAA CATATCACTT TCAAGCAGAA GATGAGCAGG ATTATATAGC GTGGATATCA 
GTACTGACGA ATAGCAAAGA AGAGGCCCTA ACCATGGCCT TCCGTGGTGA GCAGAGCACA 
GGGGAGAATA GCCTGGAGGA CCTTACCAAA GCCATCATCG AGGATGTACA GCGGCTCCCT 
GGGAATGACA TCTGCTGTGA CTGCGGCTCA TCAGAACCCA CGTGGCTTTC AACCAACTTG 
GGTATTTTGA CCTGTATAGA ATGTTCCGGA ATCCATAGGG AAATGGGGGT TCATATTTCT 
CGCATTCAGT CTTTGGAACT AGACAAATTA GGAACTTCCG AACTCTTGCT GGCCAAGAAT 
GTAGGAAACA ATAGTTTTAA TGATATTATG GAAGCAAATT TACCCAGCCC TTCACCAAAG 
CCCACCCCTT CAAGTGATAT GACCGTGCGT AAAGAGTACA TCACTGCAAA GTATGTCGAC 
CACCGCTTCT CCAGGAAGAC CTGTGCCTCT TCCTCAGCAA AGCTGAACGA ATTGCTTGAG 
GCCATCAAGT CCAGGGACTT ACTTGCATTA ATCCAAGTCT ATGCAGAGGG GGTGGAGTTA 
ATGGAGCCAC TGCTGGAACC TGGGCAGGAG CTCGGGGAGA CAGCCCTTCA TCTTGCCGTC 
AGGACCGCAG ACCAGACATC TCTCCATCTG GTTGATTTCC TTGTACAAAA CTGTGGGAAC 
CTGGATAAGC AAACGTCTGT GGGGAATACA GTTCTGCACT ATTGCAGCAT GTACGGCAAG 
CCGGAGTGTC TGAAGCTGCT GCTTAGGAGC AAGCCCACCG TGGACATCGT TAACCAGAAT 
GGAGAAACTG CCTTGGATAT AGCAAAGAGA CTCAAAGCTA CCCAGTGTGA AGATCTGCTT 
TCCCAGGCTA AATCTGGAAA GTTCAACCCT CATGTCCACG TAGAGTATGA GTGGAATCTT 
CGACAGGACG AGATGGATGA GAGCGATGAC GATCTGGATG ACAAGCCAAG CCCGATCAAA 
AAGGAACGCT CGCCCAGACC ACAGAGCTTC TGCCACTCCT CCAGCATCTC GCCACAGGAC 
AAGCTGGCAC TGCCAGGGTT CAGCACTCCA CGGGACAAGC AGCGGCTCTC CTACGGAGCC 
TTCACCAACC AGATCTTCGC CTCTACGAGC ACAGATTTGC CCACATCACC CACCAGTGAG 
GCTCCCCCTT TGCCACCTCG GAACGCCGGG AAAGGTCCAA CTGGCCCACC TTCAACACTC 
CCTCTAGGCA CCCAGACCTC TAGTGGCAGC TCCACCCTAT CCAAGAAGAG GCCTCCTCCC 
CCACCACCAG GACACAAGAG AACCCTGTCT GACCCTCCCA GCCCACTACC TCACGGGCCC 
CCAAACAAAG GCGCAATTCC TTGGGGTAAT GATGTGGGCC CATTATCTTC AAGTAAGACG 
GCCAACAAGT TTGAGGGGCT GTCTCAGCAA GCAAGCACCA GTTCTGCTAA GACTGCCCTT 
GGCCCGAGAG TGCTTCCTAA ACTACCTCAG AAAGTGGCAC TAAGGAAGAC GGAGACCAGC 
CATCATCTCT CCCTCGACAG AACCAACATC CCACCTGAGA CTTTTCAGAA ATCATCACAG 
TTGACAGAGT TACCCCAAAA GCCACCACTT GGAGAGCTGC CCCCGAAGCC TGTGGAACTG 
GCCCCCAAGC CCCAAGTTGG AGAGCTGCCA CCTAAGCCTG GAGAGCTACC CCCTAAGCCC 
CAATTAGGTG ACCTGCCCCC CAAGCCACAG CTCTCAGACT TACCTCCCAA GCCACAGATG 
AAGGACCTGC CCCCTAAGCC GCAGCTGGGG GATCTGCTGG CAAAGTCCCA GGCTGGCGAT 
GTCTCAGCCA AGGTGCAGCC ACCCTCAGAG GTCACACAGA GGTCACACAC CGGGGATCTG 
TCTCCAAATG TACAGTCCAG AGATGCCATC CAGAAGCAAG CATCTGAAGA CTCCAACGAC 
CTCACACCCA CGCTGCCAGA GACACCCGTA CCACTGCCGA GAAAAATCAA TACGGGGAAA 
AATAAAGTGA GGCGGGTGAA GACCATTTAT GACTGCCAGG CAGATAATGA CGACGAACTC 
ACATTTATTG AGGGGGAAGT GATCATTGTC ACCGGGGAAG AGGACCAGGA GTGGTGGATC 
GGGCATATCG AAGGACAGCC TGAAAGGAAG GGTGTCTTTC CAGTGTCCTT TGTCCACATC 
CTGTCTGACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asap1 ( XM_006520399.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006520462.1 Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OMu50920
Clone ID Related Accession (Same CDS sequence)	XM_006520399.4 , XM_006520399.3
Accession Version	XM_006520399.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006520399.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCAG GTCTGAGTGG GCCACAGGGC TTGCCTTCCA AAGCAGGCTT TAATGGAGGC 
CAGTCATACG CTTTCAAGGG CTCCTGGAGG CCAATCCCAA GGACTAGGAT GCCGGACCAG 
ATCTCCGTGT CCGAGTTCAT CGCCGAGACC ACCGAGGACT ACAACTCGCC CACCACGTCC 
AGCTTCACCA CGCGGCTGCA CAACTGCAGG AACACCGTCA CGCTGCTGGA GGAGGCTCTA 
GACCAAGATA GAACAGCCCT ACAGAAAGTG AAGAAATCTG TAAAAGCAAT ATATAATTCC 
GGTCAAGATC ATGTACAAAA TGAAGAAAAC TATGCACAAG TTCTGGATAA GTTTGGAAGT 
AATTTTTTAA GCAGAGACAA CCCTGACCTT GGCACCGCTT TTGTCAAGTT TTCTACACTC 
ACAAAGGAAC TGTCCACGCT GCTGAAAAAT CTGCTCCAGG GTCTGAGCCA CAATGTGATC 
TTCACCTTGG ATTCCTTGTT AAAAGGAGAC TTGAAGGGGG TCAAAGGAGA TCTCAAGAAG 
CCATTTGACA AAGCCTGGAA AGATTATGAG ACGAAGTTTA CAAAAATTGA GAAGGAGAAA 
AGAGAGCACG CAAAACAGCA CGGGATGATC CGAACAGAGA TAACAGGCGC TGAGATTGCA 
GAAGAAATGG AAAAGGAACG GCGGCTTTTT CAGCTCCAGA TGTGTGAATA TCTCATTAAA 
GTAAATGAAA TCAAGACCAA AAAGGGTGTG GACCTGCTGC AGAACCTTAT AAAGTATTAC 
CATGCACAGT GCAATTTCTT TCAGGATGGC TTGAAAACGG CTGATAAACT GAAACAGTAC 
ATAGAAAAGC TGGCTGCTGA CTTATATAAT ATAAAGCAAA CCCAAGATGA AGAAAAAAAA 
CAGCTAACTG CACTCCGAGA TCTAATAAAA TCCTCTCTGC AACTTGATCC GAAAGAAGTA 
GGTGGTTTAT ATGTTGCCAG CAGGGCTAAC AGTGACTCTC AGAGCCGGCA AGGTGGGTAC 
AGCATGCATC AGCTCCAGGG CAACAAGGAG TACGGCAGTG AAAAGAAGGG CTTCCTGCTG 
AAGAAGAGTG ATGGGATCCG GAAAGTGTGG CAGAGACGGA AGTGTGCGGT CAAGAACGGG 
ATCCTGACCA TCTCCCACGC AACTTCCAAC AGGCAGCCTG CTAAGTTAAA CCTCCTCACC 
TGCCAAGTGA AACCGAATGC TGAGGACAAG AAGTCTTTTG ACCTGATATC ACATAATCGA 
ACATATCACT TTCAAGCAGA AGATGAGCAG GATTATATAG CGTGGATATC AGTACTGACG 
AATAGCAAAG AAGAGGCCCT AACCATGGCC TTCCGTGGTG AGCAGAGCAC AGGGGAGAAT 
AGCCTGGAGG ACCTTACCAA AGCCATCATC GAGGATGTAC AGCGGCTCCC TGGGAATGAC 
ATCTGCTGTG ACTGCGGCTC ATCAGAACCC ACGTGGCTTT CAACCAACTT GGGTATTTTG 
ACCTGTATAG AATGTTCCGG AATCCATAGG GAAATGGGGG TTCATATTTC TCGCATTCAG 
TCTTTGGAAC TAGACAAATT AGGAACTTCC GAACTCTTGC TGGCCAAGAA TGTAGGAAAC 
AATAGTTTTA ATGATATTAT GGAAGCAAAT TTACCCAGCC CTTCACCAAA GCCCACCCCT 
TCAAGTGATA TGACCGTGCG TAAAGAGTAC ATCACTGCAA AGTATGTCGA CCACCGCTTC 
TCCAGGAAGA CCTGTGCCTC TTCCTCAGCA AAGCTGAACG AATTGCTTGA GGCCATCAAG 
TCCAGGGACT TACTTGCATT AATCCAAGTC TATGCAGAGG GGGTGGAGTT AATGGAGCCA 
CTGCTGGAAC CTGGGCAGGA GCTCGGGGAG ACAGCCCTTC ATCTTGCCGT CAGGACCGCA 
GACCAGACAT CTCTCCATCT GGTTGATTTC CTTGTACAAA ACTGTGGGAA CCTGGATAAG 
CAAACGTCTG TGGGGAATAC AGTTCTGCAC TATTGCAGCA TGTACGGCAA GCCGGAGTGT 
CTGAAGCTGC TGCTTAGGAG CAAGCCCACC GTGGACATCG TTAACCAGAA TGGAGAAACT 
GCCTTGGATA TAGCAAAGAG ACTCAAAGCT ACCCAGTGTG AAGATCTGCT TTCCCAGGCT 
AAATCTGGAA AGTTCAACCC TCATGTCCAC GTAGAGTATG AGTGGAATCT TCGACAGGAC 
GAGATGGATG AGAGCGATGA CGATCTGGAT GACAAGCCAA GCCCGATCAA AAAGGAACGC 
TCGCCCAGAC CACAGAGCTT CTGCCACTCC TCCAGCATCT CGCCACAGGA CAAGCTGGCA 
CTGCCAGGGT TCAGCACTCC ACGGGACAAG CAGCGGCTCT CCTACGGAGC CTTCACCAAC 
CAGATCTTCG CCTCTACGAG CACAGATTTG CCCACATCAC CCACCAGTGA GGCTCCCCCT 
TTGCCACCTC GGAACGCCGG GAAAGGTCCA ACTGGCCCAC CTTCAACACT CCCTCTAGGC 
ACCCAGACCT CTAGTGGCAG CTCCACCCTA TCCAAGAAGA GGCCTCCTCC CCCACCACCA 
GGACACAAGA GAACCCTGTC TGACCCTCCC AGCCCACTAC CTCACGGGCC CCCAAACAAA 
GGCGCAATTC CTTGGGGTAA TGATGTGGGC CCATTATCTT CAAGTAAGAC GGCCAACAAG 
TTTGAGGGGC TGTCTCAGCA AGCAAGCACC AGTTCTGCTA AGACTGCCCT TGGCCCGAGA 
GTGCTTCCTA AACTACCTCA GAAAGTGGCA CTAAGGAAGA CGGAGACCAG CCATCATCTC 
TCCCTCGACA GAACCAACAT CCCACCTGAG ACTTTTCAGA AATCATCACA GTTGACAGAG 
TTACCCCAAA AGCCACCACT TGGAGAGCTG CCCCCGAAGC CTGTGGAACT GGCCCCCAAG 
CCCCAAGTTG GAGAGCTGCC ACCTAAGCCT GGAGAGCTAC CCCCTAAGCC CCAATTAGGT 
GACCTGCCCC CCAAGCCACA GCTCTCAGAC TTACCTCCCA AGCCACAGAT GAAGGACCTG 
CCCCCTAAGC CGCAGCTGGG GGATCTGCTG GCAAAGTCCC AGGCTGGCGA TGTCTCAGCC 
AAGGTGCAGC CACCCTCAGA GGTCACACAG AGGTCACACA CCGGGGATCT GTCTCCAAAT 
GTACAGTCCA GAGATGCCAT CCAGAAGCAA GCATCTGAAG ACTCCAACGA CCTCACACCC 
ACGCTGCCAG AGACACCCGT ACCACTGCCG AGAAAAATCA ATACGGGGAA AAATAAAGTG 
AGGCGGGTGA AGACCATTTA TGACTGCCAG GCAGATAATG ACGACGAACT CACATTTATT 
GAGGGGGAAG TGATCATTGT CACCGGGGAA GAGGACCAGG AGTGGTGGAT CGGGCATATC 
GAAGGACAGC CTGAAAGGAA GGGTGTCTTT CCAGTGTCCT TTGTCCACAT CCTGTCTGAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006520462.1
CDS	198..3680
Translation	MQAGLSGPQGLPSKAGFNGGQSYAFKGSWRPIPRTRMPDQISVSEFIAETTEDYNSPTTSSFTTRLHNCRNTVTLLEEALDQDRTALQKVKKSVKAIYNSGQDHVQNEENYAQVLDKFGSNFLSRDNPDLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGVKGDLKKPFDKAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDPKEVGGLYVASRANSDSQSRQGGYSMHQLQGNKEYGSEKKGFLLKKSDGIRKVWQRRKCAVKNGILTISHATSNRQPAKLNLLTCQVKPNAEDKKSFDLISHNRTYHFQAEDEQDYIAWISVLTNSKEEALTMAFRGEQSTGENSLEDLTKAIIEDVQRLPGNDICCDCGSSEPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDKLGTSELLLAKNVGNNSFNDIMEANLPSPSPKPTPSSDMTVRKEYITAKYVDHRFSRKTCASSSAKLNELLEAIKSRDLLALIQVYAEGVELMEPLLEPGQELGETALHLAVRTADQTSLHLVDFLVQNCGNLDKQTSVGNTVLHYCSMYGKPECLKLLLRSKPTVDIVNQNGETALDIAKRLKATQCEDLLSQAKSGKFNPHVHVEYEWNLRQDEMDESDDDLDDKPSPIKKERSPRPQSFCHSSSISPQDKLALPGFSTPRDKQRLSYGAFTNQIFASTSTDLPTSPTSEAPPLPPRNAGKGPTGPPSTLPLGTQTSSGSSTLSKKRPPPPPPGHKRTLSDPPSPLPHGPPNKGAIPWGNDVGPLSSSKTANKFEGLSQQASTSSAKTALGPRVLPKLPQKVALRKTETSHHLSLDRTNIPPETFQKSSQLTELPQKPPLGELPPKPVELAPKPQVGELPPKPGELPPKPQLGDLPPKPQLSDLPPKPQMKDLPPKPQLGDLLAKSQAGDVSAKVQPPSEVTQRSHTGDLSPNVQSRDAIQKQASEDSNDLTPTLPETPVPLPRKINTGKNKVRRVKTIYDCQADNDDELTFIEGEVIIVTGEEDQEWWIGHIEGQPERKGVFPVSFVHILSD

Target ORF information:

RefSeq Version	XM_006520399.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006520399.3

ORF Insert Sequence:

ATGCAGGCAG GTCTGAGTGG GCCACAGGGC TTGCCTTCCA AAGCAGGCTT TAATGGAGGC 
CAGTCATACG CTTTCAAGGG CTCCTGGAGG CCAATCCCAA GGACTAGGAT GCCGGACCAG 
ATCTCCGTGT CCGAGTTCAT CGCCGAGACC ACCGAGGACT ACAACTCGCC CACCACGTCC 
AGCTTCACCA CGCGGCTGCA CAACTGCAGG AACACCGTCA CGCTGCTGGA GGAGGCTCTA 
GACCAAGATA GAACAGCCCT ACAGAAAGTG AAGAAATCTG TAAAAGCAAT ATATAATTCC 
GGTCAAGATC ATGTACAAAA TGAAGAAAAC TATGCACAAG TTCTGGATAA GTTTGGAAGT 
AATTTTTTAA GCAGAGACAA CCCTGACCTT GGCACCGCTT TTGTCAAGTT TTCTACACTC 
ACAAAGGAAC TGTCCACGCT GCTGAAAAAT CTGCTCCAGG GTCTGAGCCA CAATGTGATC 
TTCACCTTGG ATTCCTTGTT AAAAGGAGAC TTGAAGGGGG TCAAAGGAGA TCTCAAGAAG 
CCATTTGACA AAGCCTGGAA AGATTATGAG ACGAAGTTTA CAAAAATTGA GAAGGAGAAA 
AGAGAGCACG CAAAACAGCA CGGGATGATC CGAACAGAGA TAACAGGCGC TGAGATTGCA 
GAAGAAATGG AAAAGGAACG GCGGCTTTTT CAGCTCCAGA TGTGTGAATA TCTCATTAAA 
GTAAATGAAA TCAAGACCAA AAAGGGTGTG GACCTGCTGC AGAACCTTAT AAAGTATTAC 
CATGCACAGT GCAATTTCTT TCAGGATGGC TTGAAAACGG CTGATAAACT GAAACAGTAC 
ATAGAAAAGC TGGCTGCTGA CTTATATAAT ATAAAGCAAA CCCAAGATGA AGAAAAAAAA 
CAGCTAACTG CACTCCGAGA TCTAATAAAA TCCTCTCTGC AACTTGATCC GAAAGAAGTA 
GGTGGTTTAT ATGTTGCCAG CAGGGCTAAC AGTGACTCTC AGAGCCGGCA AGGTGGGTAC 
AGCATGCATC AGCTCCAGGG CAACAAGGAG TACGGCAGTG AAAAGAAGGG CTTCCTGCTG 
AAGAAGAGTG ATGGGATCCG GAAAGTGTGG CAGAGACGGA AGTGTGCGGT CAAGAACGGG 
ATCCTGACCA TCTCCCACGC AACTTCCAAC AGGCAGCCTG CTAAGTTAAA CCTCCTCACC 
TGCCAAGTGA AACCGAATGC TGAGGACAAG AAGTCTTTTG ACCTGATATC ACATAATCGA 
ACATATCACT TTCAAGCAGA AGATGAGCAG GATTATATAG CGTGGATATC AGTACTGACG 
AATAGCAAAG AAGAGGCCCT AACCATGGCC TTCCGTGGTG AGCAGAGCAC AGGGGAGAAT 
AGCCTGGAGG ACCTTACCAA AGCCATCATC GAGGATGTAC AGCGGCTCCC TGGGAATGAC 
ATCTGCTGTG ACTGCGGCTC ATCAGAACCC ACGTGGCTTT CAACCAACTT GGGTATTTTG 
ACCTGTATAG AATGTTCCGG AATCCATAGG GAAATGGGGG TTCATATTTC TCGCATTCAG 
TCTTTGGAAC TAGACAAATT AGGAACTTCC GAACTCTTGC TGGCCAAGAA TGTAGGAAAC 
AATAGTTTTA ATGATATTAT GGAAGCAAAT TTACCCAGCC CTTCACCAAA GCCCACCCCT 
TCAAGTGATA TGACCGTGCG TAAAGAGTAC ATCACTGCAA AGTATGTCGA CCACCGCTTC 
TCCAGGAAGA CCTGTGCCTC TTCCTCAGCA AAGCTGAACG AATTGCTTGA GGCCATCAAG 
TCCAGGGACT TACTTGCATT AATCCAAGTC TATGCAGAGG GGGTGGAGTT AATGGAGCCA 
CTGCTGGAAC CTGGGCAGGA GCTCGGGGAG ACAGCCCTTC ATCTTGCCGT CAGGACCGCA 
GACCAGACAT CTCTCCATCT GGTTGATTTC CTTGTACAAA ACTGTGGGAA CCTGGATAAG 
CAAACGTCTG TGGGGAATAC AGTTCTGCAC TATTGCAGCA TGTACGGCAA GCCGGAGTGT 
CTGAAGCTGC TGCTTAGGAG CAAGCCCACC GTGGACATCG TTAACCAGAA TGGAGAAACT 
GCCTTGGATA TAGCAAAGAG ACTCAAAGCT ACCCAGTGTG AAGATCTGCT TTCCCAGGCT 
AAATCTGGAA AGTTCAACCC TCATGTCCAC GTAGAGTATG AGTGGAATCT TCGACAGGAC 
GAGATGGATG AGAGCGATGA CGATCTGGAT GACAAGCCAA GCCCGATCAA AAAGGAACGC 
TCGCCCAGAC CACAGAGCTT CTGCCACTCC TCCAGCATCT CGCCACAGGA CAAGCTGGCA 
CTGCCAGGGT TCAGCACTCC ACGGGACAAG CAGCGGCTCT CCTACGGAGC CTTCACCAAC 
CAGATCTTCG CCTCTACGAG CACAGATTTG CCCACATCAC CCACCAGTGA GGCTCCCCCT 
TTGCCACCTC GGAACGCCGG GAAAGGTCCA ACTGGCCCAC CTTCAACACT CCCTCTAGGC 
ACCCAGACCT CTAGTGGCAG CTCCACCCTA TCCAAGAAGA GGCCTCCTCC CCCACCACCA 
GGACACAAGA GAACCCTGTC TGACCCTCCC AGCCCACTAC CTCACGGGCC CCCAAACAAA 
GGCGCAATTC CTTGGGGTAA TGATGTGGGC CCATTATCTT CAAGTAAGAC GGCCAACAAG 
TTTGAGGGGC TGTCTCAGCA AGCAAGCACC AGTTCTGCTA AGACTGCCCT TGGCCCGAGA 
GTGCTTCCTA AACTACCTCA GAAAGTGGCA CTAAGGAAGA CGGAGACCAG CCATCATCTC 
TCCCTCGACA GAACCAACAT CCCACCTGAG ACTTTTCAGA AATCATCACA GTTGACAGAG 
TTACCCCAAA AGCCACCACT TGGAGAGCTG CCCCCGAAGC CTGTGGAACT GGCCCCCAAG 
CCCCAAGTTG GAGAGCTGCC ACCTAAGCCT GGAGAGCTAC CCCCTAAGCC CCAATTAGGT 
GACCTGCCCC CCAAGCCACA GCTCTCAGAC TTACCTCCCA AGCCACAGAT GAAGGACCTG 
CCCCCTAAGC CGCAGCTGGG GGATCTGCTG GCAAAGTCCC AGGCTGGCGA TGTCTCAGCC 
AAGGTGCAGC CACCCTCAGA GGTCACACAG AGGTCACACA CCGGGGATCT GTCTCCAAAT 
GTACAGTCCA GAGATGCCAT CCAGAAGCAA GCATCTGAAG ACTCCAACGA CCTCACACCC 
ACGCTGCCAG AGACACCCGT ACCACTGCCG AGAAAAATCA ATACGGGGAA AAATAAAGTG 
AGGCGGGTGA AGACCATTTA TGACTGCCAG GCAGATAATG ACGACGAACT CACATTTATT 
GAGGGGGAAG TGATCATTGT CACCGGGGAA GAGGACCAGG AGTGGTGGAT CGGGCATATC 
GAAGGACAGC CTGAAAGGAA GGGTGTCTTT CCAGTGTCCT TTGTCCACAT CCTGTCTGAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asap1 ( XM_006520400.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006520463.1 Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OMu50921
Clone ID Related Accession (Same CDS sequence)	XM_006520400.4 , XM_006520400.3
Accession Version	XM_006520400.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3456bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006520400.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCAG GTCTGAGTGG GCCACAGGGC TTGCCTTCCA AAGCAGGCTT TAATGGAGGC 
CAGTCATACG CTTTCAAGGG CTCCTGGAGG CCAATCCCAA GGACTAGGAT GCCGGACCAG 
ATCTCCGTGT CCGAGTTCAT CGCCGAGACC ACCGAGGACT ACAACTCGCC CACCACGTCC 
AGCTTCACCA CGCGGCTGCA CAACTGCAGG AACACCGTCA CGCTGCTGGA GGAGGCTCTA 
GACCAAGATA GAACAGCCCT ACAGAAAGTG AAGAAATCTG TAAAAGCAAT ATATAATTCC 
GGTCAAGATC ATGTACAAAA TGAAGAAAAC TATGCACAAG TTCTGGATAA GTTTGGAAGT 
AATTTTTTAA GCAGAGACAA CCCTGACCTT GGCACCGCTT TTGTCAAGTT TTCTACACTC 
ACAAAGGAAC TGTCCACGCT GCTGAAAAAT CTGCTCCAGG GTCTGAGCCA CAATGTGATC 
TTCACCTTGG ATTCCTTGTT AAAAGGAGAC TTGAAGGGGG TCAAAGGAGA TCTCAAGAAG 
CCATTTGACA AAGCCTGGAA AGATTATGAG ACGAAGTTTA CAAAAATTGA GAAGGAGAAA 
AGAGAGCACG CAAAACAGCA CGGGATGATC CGAACAGAGA TAACAGGCGC TGAGATTGCA 
GAAGAAATGG AAAAGGAACG GCGGCTTTTT CAGCTCCAGA TGTGTGAATA TCTCATTAAA 
GTAAATGAAA TCAAGACCAA AAAGGGTGTG GACCTGCTGC AGAACCTTAT AAAGTATTAC 
CATGCACAGT GCAATTTCTT TCAGGATGGC TTGAAAACGG CTGATAAACT GAAACAGTAC 
ATAGAAAAGC TGGCTGCTGA CTTATATAAT ATAAAGCAAA CCCAAGATGA AGAAAAAAAA 
CAGCTAACTG CACTCCGAGA TCTAATAAAA TCCTCTCTGC AACTTGATCC GAAAGAATCT 
AGAAGAGACT CTCAGAGCCG GCAAGGTGGG TACAGCATGC ATCAGCTCCA GGGCAACAAG 
GAGTACGGCA GTGAAAAGAA GGGCTTCCTG CTGAAGAAGA GTGATGGGAT CCGGAAAGTG 
TGGCAGAGAC GGAAGTGTGC GGTCAAGAAC GGGATCCTGA CCATCTCCCA CGCAACTTCC 
AACAGGCAGC CTGCTAAGTT AAACCTCCTC ACCTGCCAAG TGAAACCGAA TGCTGAGGAC 
AAGAAGTCTT TTGACCTGAT ATCACATAAT CGAACATATC ACTTTCAAGC AGAAGATGAG 
CAGGATTATA TAGCGTGGAT ATCAGTACTG ACGAATAGCA AAGAAGAGGC CCTAACCATG 
GCCTTCCGTG GTGAGCAGAG CACAGGGGAG AATAGCCTGG AGGACCTTAC CAAAGCCATC 
ATCGAGGATG TACAGCGGCT CCCTGGGAAT GACATCTGCT GTGACTGCGG CTCATCAGAA 
CCCACGTGGC TTTCAACCAA CTTGGGTATT TTGACCTGTA TAGAATGTTC CGGAATCCAT 
AGGGAAATGG GGGTTCATAT TTCTCGCATT CAGTCTTTGG AACTAGACAA ATTAGGAACT 
TCCGAACTCT TGCTGGCCAA GAATGTAGGA AACAATAGTT TTAATGATAT TATGGAAGCA 
AATTTACCCA GCCCTTCACC AAAGCCCACC CCTTCAAGTG ATATGACCGT GCGTAAAGAG 
TACATCACTG CAAAGTATGT CGACCACCGC TTCTCCAGGA AGACCTGTGC CTCTTCCTCA 
GCAAAGCTGA ACGAATTGCT TGAGGCCATC AAGTCCAGGG ACTTACTTGC ATTAATCCAA 
GTCTATGCAG AGGGGGTGGA GTTAATGGAG CCACTGCTGG AACCTGGGCA GGAGCTCGGG 
GAGACAGCCC TTCATCTTGC CGTCAGGACC GCAGACCAGA CATCTCTCCA TCTGGTTGAT 
TTCCTTGTAC AAAACTGTGG GAACCTGGAT AAGCAAACGT CTGTGGGGAA TACAGTTCTG 
CACTATTGCA GCATGTACGG CAAGCCGGAG TGTCTGAAGC TGCTGCTTAG GAGCAAGCCC 
ACCGTGGACA TCGTTAACCA GAATGGAGAA ACTGCCTTGG ATATAGCAAA GAGACTCAAA 
GCTACCCAGT GTGAAGATCT GCTTTCCCAG GCTAAATCTG GAAAGTTCAA CCCTCATGTC 
CACGTAGAGT ATGAGTGGAA TCTTCGACAG GACGAGATGG ATGAGAGCGA TGACGATCTG 
GATGACAAGC CAAGCCCGAT CAAAAAGGAA CGCTCGCCCA GACCACAGAG CTTCTGCCAC 
TCCTCCAGCA TCTCGCCACA GGACAAGCTG GCACTGCCAG GGTTCAGCAC TCCACGGGAC 
AAGCAGCGGC TCTCCTACGG AGCCTTCACC AACCAGATCT TCGCCTCTAC GAGCACAGAT 
TTGCCCACAT CACCCACCAG TGAGGCTCCC CCTTTGCCAC CTCGGAACGC CGGGAAAGGT 
CCAACTGGCC CACCTTCAAC ACTCCCTCTA GGCACCCAGA CCTCTAGTGG CAGCTCCACC 
CTATCCAAGA AGAGGCCTCC TCCCCCACCA CCAGGACACA AGAGAACCCT GTCTGACCCT 
CCCAGCCCAC TACCTCACGG GCCCCCAAAC AAAGGCGCAA TTCCTTGGGG TAATGATGTG 
GGCCCATTAT CTTCAAGTAA GACGGCCAAC AAGTTTGAGG GGCTGTCTCA GCAAGCAAGC 
ACCAGTTCTG CTAAGACTGC CCTTGGCCCG AGAGTGCTTC CTAAACTACC TCAGAAAGTG 
GCACTAAGGA AGACGGAGAC CAGCCATCAT CTCTCCCTCG ACAGAACCAA CATCCCACCT 
GAGACTTTTC AGAAATCATC ACAGTTGACA GAGTTACCCC AAAAGCCACC ACTTGGAGAG 
CTGCCCCCGA AGCCTGTGGA ACTGGCCCCC AAGCCCCAAG TTGGAGAGCT GCCACCTAAG 
CCTGGAGAGC TACCCCCTAA GCCCCAATTA GGTGACCTGC CCCCCAAGCC ACAGCTCTCA 
GACTTACCTC CCAAGCCACA GATGAAGGAC CTGCCCCCTA AGCCGCAGCT GGGGGATCTG 
CTGGCAAAGT CCCAGGCTGG CGATGTCTCA GCCAAGGTGC AGCCACCCTC AGAGGTCACA 
CAGAGGTCAC ACACCGGGGA TCTGTCTCCA AATGTACAGT CCAGAGATGC CATCCAGAAG 
CAAGCATCTG AAGACTCCAA CGACCTCACA CCCACGCTGC CAGAGACACC CGTACCACTG 
CCGAGAAAAA TCAATACGGG GAAAAATAAA GTGAGGCGGG TGAAGACCAT TTATGACTGC 
CAGGCAGATA ATGACGACGA ACTCACATTT ATTGAGGGGG AAGTGATCAT TGTCACCGGG 
GAAGAGGACC AGGAGTGGTG GATCGGGCAT ATCGAAGGAC AGCCTGAAAG GAAGGGTGTC 
TTTCCAGTGT CCTTTGTCCA CATCCTGTCT GACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006520463.1
CDS	198..3653
Translation	MQAGLSGPQGLPSKAGFNGGQSYAFKGSWRPIPRTRMPDQISVSEFIAETTEDYNSPTTSSFTTRLHNCRNTVTLLEEALDQDRTALQKVKKSVKAIYNSGQDHVQNEENYAQVLDKFGSNFLSRDNPDLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGVKGDLKKPFDKAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDPKESRRDSQSRQGGYSMHQLQGNKEYGSEKKGFLLKKSDGIRKVWQRRKCAVKNGILTISHATSNRQPAKLNLLTCQVKPNAEDKKSFDLISHNRTYHFQAEDEQDYIAWISVLTNSKEEALTMAFRGEQSTGENSLEDLTKAIIEDVQRLPGNDICCDCGSSEPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDKLGTSELLLAKNVGNNSFNDIMEANLPSPSPKPTPSSDMTVRKEYITAKYVDHRFSRKTCASSSAKLNELLEAIKSRDLLALIQVYAEGVELMEPLLEPGQELGETALHLAVRTADQTSLHLVDFLVQNCGNLDKQTSVGNTVLHYCSMYGKPECLKLLLRSKPTVDIVNQNGETALDIAKRLKATQCEDLLSQAKSGKFNPHVHVEYEWNLRQDEMDESDDDLDDKPSPIKKERSPRPQSFCHSSSISPQDKLALPGFSTPRDKQRLSYGAFTNQIFASTSTDLPTSPTSEAPPLPPRNAGKGPTGPPSTLPLGTQTSSGSSTLSKKRPPPPPPGHKRTLSDPPSPLPHGPPNKGAIPWGNDVGPLSSSKTANKFEGLSQQASTSSAKTALGPRVLPKLPQKVALRKTETSHHLSLDRTNIPPETFQKSSQLTELPQKPPLGELPPKPVELAPKPQVGELPPKPGELPPKPQLGDLPPKPQLSDLPPKPQMKDLPPKPQLGDLLAKSQAGDVSAKVQPPSEVTQRSHTGDLSPNVQSRDAIQKQASEDSNDLTPTLPETPVPLPRKINTGKNKVRRVKTIYDCQADNDDELTFIEGEVIIVTGEEDQEWWIGHIEGQPERKGVFPVSFVHILSD

Target ORF information:

RefSeq Version	XM_006520400.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006520400.3

ORF Insert Sequence:

ATGCAGGCAG GTCTGAGTGG GCCACAGGGC TTGCCTTCCA AAGCAGGCTT TAATGGAGGC 
CAGTCATACG CTTTCAAGGG CTCCTGGAGG CCAATCCCAA GGACTAGGAT GCCGGACCAG 
ATCTCCGTGT CCGAGTTCAT CGCCGAGACC ACCGAGGACT ACAACTCGCC CACCACGTCC 
AGCTTCACCA CGCGGCTGCA CAACTGCAGG AACACCGTCA CGCTGCTGGA GGAGGCTCTA 
GACCAAGATA GAACAGCCCT ACAGAAAGTG AAGAAATCTG TAAAAGCAAT ATATAATTCC 
GGTCAAGATC ATGTACAAAA TGAAGAAAAC TATGCACAAG TTCTGGATAA GTTTGGAAGT 
AATTTTTTAA GCAGAGACAA CCCTGACCTT GGCACCGCTT TTGTCAAGTT TTCTACACTC 
ACAAAGGAAC TGTCCACGCT GCTGAAAAAT CTGCTCCAGG GTCTGAGCCA CAATGTGATC 
TTCACCTTGG ATTCCTTGTT AAAAGGAGAC TTGAAGGGGG TCAAAGGAGA TCTCAAGAAG 
CCATTTGACA AAGCCTGGAA AGATTATGAG ACGAAGTTTA CAAAAATTGA GAAGGAGAAA 
AGAGAGCACG CAAAACAGCA CGGGATGATC CGAACAGAGA TAACAGGCGC TGAGATTGCA 
GAAGAAATGG AAAAGGAACG GCGGCTTTTT CAGCTCCAGA TGTGTGAATA TCTCATTAAA 
GTAAATGAAA TCAAGACCAA AAAGGGTGTG GACCTGCTGC AGAACCTTAT AAAGTATTAC 
CATGCACAGT GCAATTTCTT TCAGGATGGC TTGAAAACGG CTGATAAACT GAAACAGTAC 
ATAGAAAAGC TGGCTGCTGA CTTATATAAT ATAAAGCAAA CCCAAGATGA AGAAAAAAAA 
CAGCTAACTG CACTCCGAGA TCTAATAAAA TCCTCTCTGC AACTTGATCC GAAAGAATCT 
AGAAGAGACT CTCAGAGCCG GCAAGGTGGG TACAGCATGC ATCAGCTCCA GGGCAACAAG 
GAGTACGGCA GTGAAAAGAA GGGCTTCCTG CTGAAGAAGA GTGATGGGAT CCGGAAAGTG 
TGGCAGAGAC GGAAGTGTGC GGTCAAGAAC GGGATCCTGA CCATCTCCCA CGCAACTTCC 
AACAGGCAGC CTGCTAAGTT AAACCTCCTC ACCTGCCAAG TGAAACCGAA TGCTGAGGAC 
AAGAAGTCTT TTGACCTGAT ATCACATAAT CGAACATATC ACTTTCAAGC AGAAGATGAG 
CAGGATTATA TAGCGTGGAT ATCAGTACTG ACGAATAGCA AAGAAGAGGC CCTAACCATG 
GCCTTCCGTG GTGAGCAGAG CACAGGGGAG AATAGCCTGG AGGACCTTAC CAAAGCCATC 
ATCGAGGATG TACAGCGGCT CCCTGGGAAT GACATCTGCT GTGACTGCGG CTCATCAGAA 
CCCACGTGGC TTTCAACCAA CTTGGGTATT TTGACCTGTA TAGAATGTTC CGGAATCCAT 
AGGGAAATGG GGGTTCATAT TTCTCGCATT CAGTCTTTGG AACTAGACAA ATTAGGAACT 
TCCGAACTCT TGCTGGCCAA GAATGTAGGA AACAATAGTT TTAATGATAT TATGGAAGCA 
AATTTACCCA GCCCTTCACC AAAGCCCACC CCTTCAAGTG ATATGACCGT GCGTAAAGAG 
TACATCACTG CAAAGTATGT CGACCACCGC TTCTCCAGGA AGACCTGTGC CTCTTCCTCA 
GCAAAGCTGA ACGAATTGCT TGAGGCCATC AAGTCCAGGG ACTTACTTGC ATTAATCCAA 
GTCTATGCAG AGGGGGTGGA GTTAATGGAG CCACTGCTGG AACCTGGGCA GGAGCTCGGG 
GAGACAGCCC TTCATCTTGC CGTCAGGACC GCAGACCAGA CATCTCTCCA TCTGGTTGAT 
TTCCTTGTAC AAAACTGTGG GAACCTGGAT AAGCAAACGT CTGTGGGGAA TACAGTTCTG 
CACTATTGCA GCATGTACGG CAAGCCGGAG TGTCTGAAGC TGCTGCTTAG GAGCAAGCCC 
ACCGTGGACA TCGTTAACCA GAATGGAGAA ACTGCCTTGG ATATAGCAAA GAGACTCAAA 
GCTACCCAGT GTGAAGATCT GCTTTCCCAG GCTAAATCTG GAAAGTTCAA CCCTCATGTC 
CACGTAGAGT ATGAGTGGAA TCTTCGACAG GACGAGATGG ATGAGAGCGA TGACGATCTG 
GATGACAAGC CAAGCCCGAT CAAAAAGGAA CGCTCGCCCA GACCACAGAG CTTCTGCCAC 
TCCTCCAGCA TCTCGCCACA GGACAAGCTG GCACTGCCAG GGTTCAGCAC TCCACGGGAC 
AAGCAGCGGC TCTCCTACGG AGCCTTCACC AACCAGATCT TCGCCTCTAC GAGCACAGAT 
TTGCCCACAT CACCCACCAG TGAGGCTCCC CCTTTGCCAC CTCGGAACGC CGGGAAAGGT 
CCAACTGGCC CACCTTCAAC ACTCCCTCTA GGCACCCAGA CCTCTAGTGG CAGCTCCACC 
CTATCCAAGA AGAGGCCTCC TCCCCCACCA CCAGGACACA AGAGAACCCT GTCTGACCCT 
CCCAGCCCAC TACCTCACGG GCCCCCAAAC AAAGGCGCAA TTCCTTGGGG TAATGATGTG 
GGCCCATTAT CTTCAAGTAA GACGGCCAAC AAGTTTGAGG GGCTGTCTCA GCAAGCAAGC 
ACCAGTTCTG CTAAGACTGC CCTTGGCCCG AGAGTGCTTC CTAAACTACC TCAGAAAGTG 
GCACTAAGGA AGACGGAGAC CAGCCATCAT CTCTCCCTCG ACAGAACCAA CATCCCACCT 
GAGACTTTTC AGAAATCATC ACAGTTGACA GAGTTACCCC AAAAGCCACC ACTTGGAGAG 
CTGCCCCCGA AGCCTGTGGA ACTGGCCCCC AAGCCCCAAG TTGGAGAGCT GCCACCTAAG 
CCTGGAGAGC TACCCCCTAA GCCCCAATTA GGTGACCTGC CCCCCAAGCC ACAGCTCTCA 
GACTTACCTC CCAAGCCACA GATGAAGGAC CTGCCCCCTA AGCCGCAGCT GGGGGATCTG 
CTGGCAAAGT CCCAGGCTGG CGATGTCTCA GCCAAGGTGC AGCCACCCTC AGAGGTCACA 
CAGAGGTCAC ACACCGGGGA TCTGTCTCCA AATGTACAGT CCAGAGATGC CATCCAGAAG 
CAAGCATCTG AAGACTCCAA CGACCTCACA CCCACGCTGC CAGAGACACC CGTACCACTG 
CCGAGAAAAA TCAATACGGG GAAAAATAAA GTGAGGCGGG TGAAGACCAT TTATGACTGC 
CAGGCAGATA ATGACGACGA ACTCACATTT ATTGAGGGGG AAGTGATCAT TGTCACCGGG 
GAAGAGGACC AGGAGTGGTG GATCGGGCAT ATCGAAGGAC AGCCTGAAAG GAAGGGTGTC 
TTTCCAGTGT CCTTTGTCCA CATCCTGTCT GACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asap1 ( XM_006520401.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006520464.1 Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OMu50922
Clone ID Related Accession (Same CDS sequence)	XM_006520401.3 , XM_006520401.4
Accession Version	XM_006520401.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3447bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006520401.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCAG GTCTGAGTGG GCCACAGGGC TTGCCTTCCA AAGCAGGCTT TAATGGAGGC 
CAGTCATACG CTTTCAAGGG CTCCTGGAGG CCAATCCCAA GGACTAGGAT GCCGGACCAG 
ATCTCCGTGT CCGAGTTCAT CGCCGAGACC ACCGAGGACT ACAACTCGCC CACCACGTCC 
AGCTTCACCA CGCGGCTGCA CAACTGCAGG AACACCGTCA CGCTGCTGGA GGAGGCTCTA 
GACCAAGATA GAACAGCCCT ACAGAAAGTG AAGAAATCTG TAAAAGCAAT ATATAATTCC 
GGTCAAGATC ATGTACAAAA TGAAGAAAAC TATGCACAAG TTCTGGATAA GTTTGGAAGT 
AATTTTTTAA GCAGAGACAA CCCTGACCTT GGCACCGCTT TTGTCAAGTT TTCTACACTC 
ACAAAGGAAC TGTCCACGCT GCTGAAAAAT CTGCTCCAGG GTCTGAGCCA CAATGTGATC 
TTCACCTTGG ATTCCTTGTT AAAAGGAGAC TTGAAGGGGG TCAAAGGAGA TCTCAAGAAG 
CCATTTGACA AAGCCTGGAA AGATTATGAG ACGAAGTTTA CAAAAATTGA GAAGGAGAAA 
AGAGAGCACG CAAAACAGCA CGGGATGATC CGAACAGAGA TAACAGGCGC TGAGATTGCA 
GAAGAAATGG AAAAGGAACG GCGGCTTTTT CAGCTCCAGA TGTGTGAATA TCTCATTAAA 
GTAAATGAAA TCAAGACCAA AAAGGGTGTG GACCTGCTGC AGAACCTTAT AAAGTATTAC 
CATGCACAGT GCAATTTCTT TCAGGATGGC TTGAAAACGG CTGATAAACT GAAACAGTAC 
ATAGAAAAGC TGGCTGCTGA CTTATATAAT ATAAAGCAAA CCCAAGATGA AGAAAAAAAA 
CAGCTAACTG CACTCCGAGA TCTAATAAAA TCCTCTCTGC AACTTGATCC GAAAGAAGAC 
TCTCAGAGCC GGCAAGGTGG GTACAGCATG CATCAGCTCC AGGGCAACAA GGAGTACGGC 
AGTGAAAAGA AGGGCTTCCT GCTGAAGAAG AGTGATGGGA TCCGGAAAGT GTGGCAGAGA 
CGGAAGTGTG CGGTCAAGAA CGGGATCCTG ACCATCTCCC ACGCAACTTC CAACAGGCAG 
CCTGCTAAGT TAAACCTCCT CACCTGCCAA GTGAAACCGA ATGCTGAGGA CAAGAAGTCT 
TTTGACCTGA TATCACATAA TCGAACATAT CACTTTCAAG CAGAAGATGA GCAGGATTAT 
ATAGCGTGGA TATCAGTACT GACGAATAGC AAAGAAGAGG CCCTAACCAT GGCCTTCCGT 
GGTGAGCAGA GCACAGGGGA GAATAGCCTG GAGGACCTTA CCAAAGCCAT CATCGAGGAT 
GTACAGCGGC TCCCTGGGAA TGACATCTGC TGTGACTGCG GCTCATCAGA ACCCACGTGG 
CTTTCAACCA ACTTGGGTAT TTTGACCTGT ATAGAATGTT CCGGAATCCA TAGGGAAATG 
GGGGTTCATA TTTCTCGCAT TCAGTCTTTG GAACTAGACA AATTAGGAAC TTCCGAACTC 
TTGCTGGCCA AGAATGTAGG AAACAATAGT TTTAATGATA TTATGGAAGC AAATTTACCC 
AGCCCTTCAC CAAAGCCCAC CCCTTCAAGT GATATGACCG TGCGTAAAGA GTACATCACT 
GCAAAGTATG TCGACCACCG CTTCTCCAGG AAGACCTGTG CCTCTTCCTC AGCAAAGCTG 
AACGAATTGC TTGAGGCCAT CAAGTCCAGG GACTTACTTG CATTAATCCA AGTCTATGCA 
GAGGGGGTGG AGTTAATGGA GCCACTGCTG GAACCTGGGC AGGAGCTCGG GGAGACAGCC 
CTTCATCTTG CCGTCAGGAC CGCAGACCAG ACATCTCTCC ATCTGGTTGA TTTCCTTGTA 
CAAAACTGTG GGAACCTGGA TAAGCAAACG TCTGTGGGGA ATACAGTTCT GCACTATTGC 
AGCATGTACG GCAAGCCGGA GTGTCTGAAG CTGCTGCTTA GGAGCAAGCC CACCGTGGAC 
ATCGTTAACC AGAATGGAGA AACTGCCTTG GATATAGCAA AGAGACTCAA AGCTACCCAG 
TGTGAAGATC TGCTTTCCCA GGCTAAATCT GGAAAGTTCA ACCCTCATGT CCACGTAGAG 
TATGAGTGGA ATCTTCGACA GGACGAGATG GATGAGAGCG ATGACGATCT GGATGACAAG 
CCAAGCCCGA TCAAAAAGGA ACGCTCGCCC AGACCACAGA GCTTCTGCCA CTCCTCCAGC 
ATCTCGCCAC AGGACAAGCT GGCACTGCCA GGGTTCAGCA CTCCACGGGA CAAGCAGCGG 
CTCTCCTACG GAGCCTTCAC CAACCAGATC TTCGCCTCTA CGAGCACAGA TTTGCCCACA 
TCACCCACCA GTGAGGCTCC CCCTTTGCCA CCTCGGAACG CCGGGAAAGG TCCAACTGGC 
CCACCTTCAA CACTCCCTCT AGGCACCCAG ACCTCTAGTG GCAGCTCCAC CCTATCCAAG 
AAGAGGCCTC CTCCCCCACC ACCAGGACAC AAGAGAACCC TGTCTGACCC TCCCAGCCCA 
CTACCTCACG GGCCCCCAAA CAAAGGCGCA ATTCCTTGGG GTAATGATGT GGGCCCATTA 
TCTTCAAGTA AGACGGCCAA CAAGTTTGAG GGGCTGTCTC AGCAAGCAAG CACCAGTTCT 
GCTAAGACTG CCCTTGGCCC GAGAGTGCTT CCTAAACTAC CTCAGAAAGT GGCACTAAGG 
AAGACGGAGA CCAGCCATCA TCTCTCCCTC GACAGAACCA ACATCCCACC TGAGACTTTT 
CAGAAATCAT CACAGTTGAC AGAGTTACCC CAAAAGCCAC CACTTGGAGA GCTGCCCCCG 
AAGCCTGTGG AACTGGCCCC CAAGCCCCAA GTTGGAGAGC TGCCACCTAA GCCTGGAGAG 
CTACCCCCTA AGCCCCAATT AGGTGACCTG CCCCCCAAGC CACAGCTCTC AGACTTACCT 
CCCAAGCCAC AGATGAAGGA CCTGCCCCCT AAGCCGCAGC TGGGGGATCT GCTGGCAAAG 
TCCCAGGCTG GCGATGTCTC AGCCAAGGTG CAGCCACCCT CAGAGGTCAC ACAGAGGTCA 
CACACCGGGG ATCTGTCTCC AAATGTACAG TCCAGAGATG CCATCCAGAA GCAAGCATCT 
GAAGACTCCA ACGACCTCAC ACCCACGCTG CCAGAGACAC CCGTACCACT GCCGAGAAAA 
ATCAATACGG GGAAAAATAA AGTGAGGCGG GTGAAGACCA TTTATGACTG CCAGGCAGAT 
AATGACGACG AACTCACATT TATTGAGGGG GAAGTGATCA TTGTCACCGG GGAAGAGGAC 
CAGGAGTGGT GGATCGGGCA TATCGAAGGA CAGCCTGAAA GGAAGGGTGT CTTTCCAGTG 
TCCTTTGTCC ACATCCTGTC TGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006520464.1
CDS	198..3644
Translation	MQAGLSGPQGLPSKAGFNGGQSYAFKGSWRPIPRTRMPDQISVSEFIAETTEDYNSPTTSSFTTRLHNCRNTVTLLEEALDQDRTALQKVKKSVKAIYNSGQDHVQNEENYAQVLDKFGSNFLSRDNPDLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGVKGDLKKPFDKAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDPKEDSQSRQGGYSMHQLQGNKEYGSEKKGFLLKKSDGIRKVWQRRKCAVKNGILTISHATSNRQPAKLNLLTCQVKPNAEDKKSFDLISHNRTYHFQAEDEQDYIAWISVLTNSKEEALTMAFRGEQSTGENSLEDLTKAIIEDVQRLPGNDICCDCGSSEPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDKLGTSELLLAKNVGNNSFNDIMEANLPSPSPKPTPSSDMTVRKEYITAKYVDHRFSRKTCASSSAKLNELLEAIKSRDLLALIQVYAEGVELMEPLLEPGQELGETALHLAVRTADQTSLHLVDFLVQNCGNLDKQTSVGNTVLHYCSMYGKPECLKLLLRSKPTVDIVNQNGETALDIAKRLKATQCEDLLSQAKSGKFNPHVHVEYEWNLRQDEMDESDDDLDDKPSPIKKERSPRPQSFCHSSSISPQDKLALPGFSTPRDKQRLSYGAFTNQIFASTSTDLPTSPTSEAPPLPPRNAGKGPTGPPSTLPLGTQTSSGSSTLSKKRPPPPPPGHKRTLSDPPSPLPHGPPNKGAIPWGNDVGPLSSSKTANKFEGLSQQASTSSAKTALGPRVLPKLPQKVALRKTETSHHLSLDRTNIPPETFQKSSQLTELPQKPPLGELPPKPVELAPKPQVGELPPKPGELPPKPQLGDLPPKPQLSDLPPKPQMKDLPPKPQLGDLLAKSQAGDVSAKVQPPSEVTQRSHTGDLSPNVQSRDAIQKQASEDSNDLTPTLPETPVPLPRKINTGKNKVRRVKTIYDCQADNDDELTFIEGEVIIVTGEEDQEWWIGHIEGQPERKGVFPVSFVHILSD

Target ORF information:

RefSeq Version	XM_006520401.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006520401.3

ORF Insert Sequence:

ATGCAGGCAG GTCTGAGTGG GCCACAGGGC TTGCCTTCCA AAGCAGGCTT TAATGGAGGC 
CAGTCATACG CTTTCAAGGG CTCCTGGAGG CCAATCCCAA GGACTAGGAT GCCGGACCAG 
ATCTCCGTGT CCGAGTTCAT CGCCGAGACC ACCGAGGACT ACAACTCGCC CACCACGTCC 
AGCTTCACCA CGCGGCTGCA CAACTGCAGG AACACCGTCA CGCTGCTGGA GGAGGCTCTA 
GACCAAGATA GAACAGCCCT ACAGAAAGTG AAGAAATCTG TAAAAGCAAT ATATAATTCC 
GGTCAAGATC ATGTACAAAA TGAAGAAAAC TATGCACAAG TTCTGGATAA GTTTGGAAGT 
AATTTTTTAA GCAGAGACAA CCCTGACCTT GGCACCGCTT TTGTCAAGTT TTCTACACTC 
ACAAAGGAAC TGTCCACGCT GCTGAAAAAT CTGCTCCAGG GTCTGAGCCA CAATGTGATC 
TTCACCTTGG ATTCCTTGTT AAAAGGAGAC TTGAAGGGGG TCAAAGGAGA TCTCAAGAAG 
CCATTTGACA AAGCCTGGAA AGATTATGAG ACGAAGTTTA CAAAAATTGA GAAGGAGAAA 
AGAGAGCACG CAAAACAGCA CGGGATGATC CGAACAGAGA TAACAGGCGC TGAGATTGCA 
GAAGAAATGG AAAAGGAACG GCGGCTTTTT CAGCTCCAGA TGTGTGAATA TCTCATTAAA 
GTAAATGAAA TCAAGACCAA AAAGGGTGTG GACCTGCTGC AGAACCTTAT AAAGTATTAC 
CATGCACAGT GCAATTTCTT TCAGGATGGC TTGAAAACGG CTGATAAACT GAAACAGTAC 
ATAGAAAAGC TGGCTGCTGA CTTATATAAT ATAAAGCAAA CCCAAGATGA AGAAAAAAAA 
CAGCTAACTG CACTCCGAGA TCTAATAAAA TCCTCTCTGC AACTTGATCC GAAAGAAGAC 
TCTCAGAGCC GGCAAGGTGG GTACAGCATG CATCAGCTCC AGGGCAACAA GGAGTACGGC 
AGTGAAAAGA AGGGCTTCCT GCTGAAGAAG AGTGATGGGA TCCGGAAAGT GTGGCAGAGA 
CGGAAGTGTG CGGTCAAGAA CGGGATCCTG ACCATCTCCC ACGCAACTTC CAACAGGCAG 
CCTGCTAAGT TAAACCTCCT CACCTGCCAA GTGAAACCGA ATGCTGAGGA CAAGAAGTCT 
TTTGACCTGA TATCACATAA TCGAACATAT CACTTTCAAG CAGAAGATGA GCAGGATTAT 
ATAGCGTGGA TATCAGTACT GACGAATAGC AAAGAAGAGG CCCTAACCAT GGCCTTCCGT 
GGTGAGCAGA GCACAGGGGA GAATAGCCTG GAGGACCTTA CCAAAGCCAT CATCGAGGAT 
GTACAGCGGC TCCCTGGGAA TGACATCTGC TGTGACTGCG GCTCATCAGA ACCCACGTGG 
CTTTCAACCA ACTTGGGTAT TTTGACCTGT ATAGAATGTT CCGGAATCCA TAGGGAAATG 
GGGGTTCATA TTTCTCGCAT TCAGTCTTTG GAACTAGACA AATTAGGAAC TTCCGAACTC 
TTGCTGGCCA AGAATGTAGG AAACAATAGT TTTAATGATA TTATGGAAGC AAATTTACCC 
AGCCCTTCAC CAAAGCCCAC CCCTTCAAGT GATATGACCG TGCGTAAAGA GTACATCACT 
GCAAAGTATG TCGACCACCG CTTCTCCAGG AAGACCTGTG CCTCTTCCTC AGCAAAGCTG 
AACGAATTGC TTGAGGCCAT CAAGTCCAGG GACTTACTTG CATTAATCCA AGTCTATGCA 
GAGGGGGTGG AGTTAATGGA GCCACTGCTG GAACCTGGGC AGGAGCTCGG GGAGACAGCC 
CTTCATCTTG CCGTCAGGAC CGCAGACCAG ACATCTCTCC ATCTGGTTGA TTTCCTTGTA 
CAAAACTGTG GGAACCTGGA TAAGCAAACG TCTGTGGGGA ATACAGTTCT GCACTATTGC 
AGCATGTACG GCAAGCCGGA GTGTCTGAAG CTGCTGCTTA GGAGCAAGCC CACCGTGGAC 
ATCGTTAACC AGAATGGAGA AACTGCCTTG GATATAGCAA AGAGACTCAA AGCTACCCAG 
TGTGAAGATC TGCTTTCCCA GGCTAAATCT GGAAAGTTCA ACCCTCATGT CCACGTAGAG 
TATGAGTGGA ATCTTCGACA GGACGAGATG GATGAGAGCG ATGACGATCT GGATGACAAG 
CCAAGCCCGA TCAAAAAGGA ACGCTCGCCC AGACCACAGA GCTTCTGCCA CTCCTCCAGC 
ATCTCGCCAC AGGACAAGCT GGCACTGCCA GGGTTCAGCA CTCCACGGGA CAAGCAGCGG 
CTCTCCTACG GAGCCTTCAC CAACCAGATC TTCGCCTCTA CGAGCACAGA TTTGCCCACA 
TCACCCACCA GTGAGGCTCC CCCTTTGCCA CCTCGGAACG CCGGGAAAGG TCCAACTGGC 
CCACCTTCAA CACTCCCTCT AGGCACCCAG ACCTCTAGTG GCAGCTCCAC CCTATCCAAG 
AAGAGGCCTC CTCCCCCACC ACCAGGACAC AAGAGAACCC TGTCTGACCC TCCCAGCCCA 
CTACCTCACG GGCCCCCAAA CAAAGGCGCA ATTCCTTGGG GTAATGATGT GGGCCCATTA 
TCTTCAAGTA AGACGGCCAA CAAGTTTGAG GGGCTGTCTC AGCAAGCAAG CACCAGTTCT 
GCTAAGACTG CCCTTGGCCC GAGAGTGCTT CCTAAACTAC CTCAGAAAGT GGCACTAAGG 
AAGACGGAGA CCAGCCATCA TCTCTCCCTC GACAGAACCA ACATCCCACC TGAGACTTTT 
CAGAAATCAT CACAGTTGAC AGAGTTACCC CAAAAGCCAC CACTTGGAGA GCTGCCCCCG 
AAGCCTGTGG AACTGGCCCC CAAGCCCCAA GTTGGAGAGC TGCCACCTAA GCCTGGAGAG 
CTACCCCCTA AGCCCCAATT AGGTGACCTG CCCCCCAAGC CACAGCTCTC AGACTTACCT 
CCCAAGCCAC AGATGAAGGA CCTGCCCCCT AAGCCGCAGC TGGGGGATCT GCTGGCAAAG 
TCCCAGGCTG GCGATGTCTC AGCCAAGGTG CAGCCACCCT CAGAGGTCAC ACAGAGGTCA 
CACACCGGGG ATCTGTCTCC AAATGTACAG TCCAGAGATG CCATCCAGAA GCAAGCATCT 
GAAGACTCCA ACGACCTCAC ACCCACGCTG CCAGAGACAC CCGTACCACT GCCGAGAAAA 
ATCAATACGG GGAAAAATAA AGTGAGGCGG GTGAAGACCA TTTATGACTG CCAGGCAGAT 
AATGACGACG AACTCACATT TATTGAGGGG GAAGTGATCA TTGTCACCGG GGAAGAGGAC 
CAGGAGTGGT GGATCGGGCA TATCGAAGGA CAGCCTGAAA GGAAGGGTGT CTTTCCAGTG 
TCCTTTGTCC ACATCCTGTC TGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asap1 ( XM_006520402.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006520465.1 Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OMu50923
Clone ID Related Accession (Same CDS sequence)	XM_006520402.3 , XM_006520402.4
Accession Version	XM_006520402.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3321bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006520402.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCAG GTCTGAGTGG GCCACAGGGC TTGCCTTCCA AAGCAGGCTT TAATGGAGGC 
CAGTCATACG CTTTCAAGGG CTCCTGGAGG CCAATCCCAA GGACTAGGAT GCCGGACCAG 
ATCTCCGTGT CCGAGTTCAT CGCCGAGACC ACCGAGGACT ACAACTCGCC CACCACGTCC 
AGCTTCACCA CGCGGCTGCA CAACTGCAGG AACACCGTCA CGCTGCTGGA GGAGGCTCTA 
GACCAAGATA GAACAGCCCT ACAGAAAGTG AAGAAATCTG TAAAAGCAAT ATATAATTCC 
GGTCAAGATC ATGTACAAAA TGAAGAAAAC TATGCACAAG TTCTGGATAA GTTTGGAAGT 
AATTTTTTAA GCAGAGACAA CCCTGACCTT GGCACCGCTT TTGTCAAGTT TTCTACACTC 
ACAAAGGAAC TGTCCACGCT GCTGAAAAAT CTGCTCCAGG GTCTGAGCCA CAATGTGATC 
TTCACCTTGG ATTCCTTGTT AAAAGGAGAC TTGAAGGGGG TCAAAGGAGA TCTCAAGAAG 
CCATTTGACA AAGCCTGGAA AGATTATGAG ACGAAGTTTA CAAAAATTGA GAAGGAGAAA 
AGAGAGCACG CAAAACAGCA CGGGATGATC CGAACAGAGA TAACAGGCGC TGAGATTGCA 
GAAGAAATGG AAAAGGAACG GCGGCTTTTT CAGCTCCAGA TGTGTGAATA TCTCATTAAA 
GTAAATGAAA TCAAGACCAA AAAGGGTGTG GACCTGCTGC AGAACCTTAT AAAGTATTAC 
CATGCACAGT GCAATTTCTT TCAGGATGGC TTGAAAACGG CTGATAAACT GAAACAGTAC 
ATAGAAAAGC TGGCTGCTGA CTTATATAAT ATAAAGCAAA CCCAAGATGA AGAAAAAAAA 
CAGCTAACTG CACTCCGAGA TCTAATAAAA TCCTCTCTGC AACTTGATCC GAAAGAAGTA 
GGTGGTTTAT ATGTTGCCAG CAGGGCTAAC AGTTCTAGAA GAGACTCTCA GAGCCGGCAA 
GGTGGGTACA GCATGCATCA GCTCCAGGGC AACAAGGAGT ACGGCAGTGA AAAGAAGGGC 
TTCCTGCTGA AGAAGAGTGA TGGGATCCGG AAAGTGTGGC AGAGACGGAA GTGTGCGGTC 
AAGAACGGGA TCCTGACCAT CTCCCACGCA ACTTCCAACA GGCAGCCTGC TAAGTTAAAC 
CTCCTCACCT GCCAAGTGAA ACCGAATGCT GAGGACAAGA AGTCTTTTGA CCTGATATCA 
CATAATCGAA CATATCACTT TCAAGCAGAA GATGAGCAGG ATTATATAGC GTGGATATCA 
GTACTGACGA ATAGCAAAGA AGAGGCCCTA ACCATGGCCT TCCGTGGTGA GCAGAGCACA 
GGGGAGAATA GCCTGGAGGA CCTTACCAAA GCCATCATCG AGGATGTACA GCGGCTCCCT 
GGGAATGACA TCTGCTGTGA CTGCGGCTCA TCAGAACCCA CGTGGCTTTC AACCAACTTG 
GGTATTTTGA CCTGTATAGA ATGTTCCGGA ATCCATAGGG AAATGGGGGT TCATATTTCT 
CGCATTCAGT CTTTGGAACT AGACAAATTA GGAACTTCCG AACTCTTGCT GGCCAAGAAT 
GTAGGAAACA ATAGTTTTAA TGATATTATG GAAGCAAATT TACCCAGCCC TTCACCAAAG 
CCCACCCCTT CAAGTGATAT GACCGTGCGT AAAGAGTACA TCACTGCAAA GTATGTCGAC 
CACCGCTTCT CCAGGAAGAC CTGTGCCTCT TCCTCAGCAA AGCTGAACGA ATTGCTTGAG 
GCCATCAAGT CCAGGGACTT ACTTGCATTA ATCCAAGTCT ATGCAGAGGG GGTGGAGTTA 
ATGGAGCCAC TGCTGGAACC TGGGCAGGAG CTCGGGGAGA CAGCCCTTCA TCTTGCCGTC 
AGGACCGCAG ACCAGACATC TCTCCATCTG GTTGATTTCC TTGTACAAAA CTGTGGGAAC 
CTGGATAAGC AAACGTCTGT GGGGAATACA GTTCTGCACT ATTGCAGCAT GTACGGCAAG 
CCGGAGTGTC TGAAGCTGCT GCTTAGGAGC AAGCCCACCG TGGACATCGT TAACCAGAAT 
GGAGAAACTG CCTTGGATAT AGCAAAGAGA CTCAAAGCTA CCCAGTGTGA AGATCTGCTT 
TCCCAGGCTA AATCTGGAAA GTTCAACCCT CATGTCCACG TAGAGTATGA GTGGAATCTT 
CGACAGGACG AGATGGATGA GAGCGATGAC GATCTGGATG ACAAGCCAAG CCCGATCAAA 
AAGGAACGCT CGCCCAGACC ACAGAGCTTC TGCCACTCCT CCAGCATCTC GCCACAGGAC 
AAGCTGGCAC TGCCAGGGTT CAGCACTCCA CGGGACAAGC AGCGGCTCTC CTACGGAGCC 
TTCACCAACC AGATCTTCGC CTCTACGAGC ACAGATTTGC CCACATCACC CACCAGTGAG 
GCTCCCCCTT TGCCACCTCG GAACGCCGGG AAAGGTAATG ATGTGGGCCC ATTATCTTCA 
AGTAAGACGG CCAACAAGTT TGAGGGGCTG TCTCAGCAAG CAAGCACCAG TTCTGCTAAG 
ACTGCCCTTG GCCCGAGAGT GCTTCCTAAA CTACCTCAGA AAGTGGCACT AAGGAAGACG 
GAGACCAGCC ATCATCTCTC CCTCGACAGA ACCAACATCC CACCTGAGAC TTTTCAGAAA 
TCATCACAGT TGACAGAGTT ACCCCAAAAG CCACCACTTG GAGAGCTGCC CCCGAAGCCT 
GTGGAACTGG CCCCCAAGCC CCAAGTTGGA GAGCTGCCAC CTAAGCCTGG AGAGCTACCC 
CCTAAGCCCC AATTAGGTGA CCTGCCCCCC AAGCCACAGC TCTCAGACTT ACCTCCCAAG 
CCACAGATGA AGGACCTGCC CCCTAAGCCG CAGCTGGGGG ATCTGCTGGC AAAGTCCCAG 
GCTGGCGATG TCTCAGCCAA GGTGCAGCCA CCCTCAGAGG TCACACAGAG GTCACACACC 
GGGGATCTGT CTCCAAATGT ACAGTCCAGA GATGCCATCC AGAAGCAAGC ATCTGAAGAC 
TCCAACGACC TCACACCCAC GCTGCCAGAG ACACCCGTAC CACTGCCGAG AAAAATCAAT 
ACGGGGAAAA ATAAAGTGAG GCGGGTGAAG ACCATTTATG ACTGCCAGGC AGATAATGAC 
GACGAACTCA CATTTATTGA GGGGGAAGTG ATCATTGTCA CCGGGGAAGA GGACCAGGAG 
TGGTGGATCG GGCATATCGA AGGACAGCCT GAAAGGAAGG GTGTCTTTCC AGTGTCCTTT 
GTCCACATCC TGTCTGACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006520465.1
CDS	198..3518
Translation	MQAGLSGPQGLPSKAGFNGGQSYAFKGSWRPIPRTRMPDQISVSEFIAETTEDYNSPTTSSFTTRLHNCRNTVTLLEEALDQDRTALQKVKKSVKAIYNSGQDHVQNEENYAQVLDKFGSNFLSRDNPDLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGVKGDLKKPFDKAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDPKEVGGLYVASRANSSRRDSQSRQGGYSMHQLQGNKEYGSEKKGFLLKKSDGIRKVWQRRKCAVKNGILTISHATSNRQPAKLNLLTCQVKPNAEDKKSFDLISHNRTYHFQAEDEQDYIAWISVLTNSKEEALTMAFRGEQSTGENSLEDLTKAIIEDVQRLPGNDICCDCGSSEPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDKLGTSELLLAKNVGNNSFNDIMEANLPSPSPKPTPSSDMTVRKEYITAKYVDHRFSRKTCASSSAKLNELLEAIKSRDLLALIQVYAEGVELMEPLLEPGQELGETALHLAVRTADQTSLHLVDFLVQNCGNLDKQTSVGNTVLHYCSMYGKPECLKLLLRSKPTVDIVNQNGETALDIAKRLKATQCEDLLSQAKSGKFNPHVHVEYEWNLRQDEMDESDDDLDDKPSPIKKERSPRPQSFCHSSSISPQDKLALPGFSTPRDKQRLSYGAFTNQIFASTSTDLPTSPTSEAPPLPPRNAGKGNDVGPLSSSKTANKFEGLSQQASTSSAKTALGPRVLPKLPQKVALRKTETSHHLSLDRTNIPPETFQKSSQLTELPQKPPLGELPPKPVELAPKPQVGELPPKPGELPPKPQLGDLPPKPQLSDLPPKPQMKDLPPKPQLGDLLAKSQAGDVSAKVQPPSEVTQRSHTGDLSPNVQSRDAIQKQASEDSNDLTPTLPETPVPLPRKINTGKNKVRRVKTIYDCQADNDDELTFIEGEVIIVTGEEDQEWWIGHIEGQPERKGVFPVSFVHILSD

Target ORF information:

RefSeq Version	XM_006520402.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006520402.3

ORF Insert Sequence:

ATGCAGGCAG GTCTGAGTGG GCCACAGGGC TTGCCTTCCA AAGCAGGCTT TAATGGAGGC 
CAGTCATACG CTTTCAAGGG CTCCTGGAGG CCAATCCCAA GGACTAGGAT GCCGGACCAG 
ATCTCCGTGT CCGAGTTCAT CGCCGAGACC ACCGAGGACT ACAACTCGCC CACCACGTCC 
AGCTTCACCA CGCGGCTGCA CAACTGCAGG AACACCGTCA CGCTGCTGGA GGAGGCTCTA 
GACCAAGATA GAACAGCCCT ACAGAAAGTG AAGAAATCTG TAAAAGCAAT ATATAATTCC 
GGTCAAGATC ATGTACAAAA TGAAGAAAAC TATGCACAAG TTCTGGATAA GTTTGGAAGT 
AATTTTTTAA GCAGAGACAA CCCTGACCTT GGCACCGCTT TTGTCAAGTT TTCTACACTC 
ACAAAGGAAC TGTCCACGCT GCTGAAAAAT CTGCTCCAGG GTCTGAGCCA CAATGTGATC 
TTCACCTTGG ATTCCTTGTT AAAAGGAGAC TTGAAGGGGG TCAAAGGAGA TCTCAAGAAG 
CCATTTGACA AAGCCTGGAA AGATTATGAG ACGAAGTTTA CAAAAATTGA GAAGGAGAAA 
AGAGAGCACG CAAAACAGCA CGGGATGATC CGAACAGAGA TAACAGGCGC TGAGATTGCA 
GAAGAAATGG AAAAGGAACG GCGGCTTTTT CAGCTCCAGA TGTGTGAATA TCTCATTAAA 
GTAAATGAAA TCAAGACCAA AAAGGGTGTG GACCTGCTGC AGAACCTTAT AAAGTATTAC 
CATGCACAGT GCAATTTCTT TCAGGATGGC TTGAAAACGG CTGATAAACT GAAACAGTAC 
ATAGAAAAGC TGGCTGCTGA CTTATATAAT ATAAAGCAAA CCCAAGATGA AGAAAAAAAA 
CAGCTAACTG CACTCCGAGA TCTAATAAAA TCCTCTCTGC AACTTGATCC GAAAGAAGTA 
GGTGGTTTAT ATGTTGCCAG CAGGGCTAAC AGTTCTAGAA GAGACTCTCA GAGCCGGCAA 
GGTGGGTACA GCATGCATCA GCTCCAGGGC AACAAGGAGT ACGGCAGTGA AAAGAAGGGC 
TTCCTGCTGA AGAAGAGTGA TGGGATCCGG AAAGTGTGGC AGAGACGGAA GTGTGCGGTC 
AAGAACGGGA TCCTGACCAT CTCCCACGCA ACTTCCAACA GGCAGCCTGC TAAGTTAAAC 
CTCCTCACCT GCCAAGTGAA ACCGAATGCT GAGGACAAGA AGTCTTTTGA CCTGATATCA 
CATAATCGAA CATATCACTT TCAAGCAGAA GATGAGCAGG ATTATATAGC GTGGATATCA 
GTACTGACGA ATAGCAAAGA AGAGGCCCTA ACCATGGCCT TCCGTGGTGA GCAGAGCACA 
GGGGAGAATA GCCTGGAGGA CCTTACCAAA GCCATCATCG AGGATGTACA GCGGCTCCCT 
GGGAATGACA TCTGCTGTGA CTGCGGCTCA TCAGAACCCA CGTGGCTTTC AACCAACTTG 
GGTATTTTGA CCTGTATAGA ATGTTCCGGA ATCCATAGGG AAATGGGGGT TCATATTTCT 
CGCATTCAGT CTTTGGAACT AGACAAATTA GGAACTTCCG AACTCTTGCT GGCCAAGAAT 
GTAGGAAACA ATAGTTTTAA TGATATTATG GAAGCAAATT TACCCAGCCC TTCACCAAAG 
CCCACCCCTT CAAGTGATAT GACCGTGCGT AAAGAGTACA TCACTGCAAA GTATGTCGAC 
CACCGCTTCT CCAGGAAGAC CTGTGCCTCT TCCTCAGCAA AGCTGAACGA ATTGCTTGAG 
GCCATCAAGT CCAGGGACTT ACTTGCATTA ATCCAAGTCT ATGCAGAGGG GGTGGAGTTA 
ATGGAGCCAC TGCTGGAACC TGGGCAGGAG CTCGGGGAGA CAGCCCTTCA TCTTGCCGTC 
AGGACCGCAG ACCAGACATC TCTCCATCTG GTTGATTTCC TTGTACAAAA CTGTGGGAAC 
CTGGATAAGC AAACGTCTGT GGGGAATACA GTTCTGCACT ATTGCAGCAT GTACGGCAAG 
CCGGAGTGTC TGAAGCTGCT GCTTAGGAGC AAGCCCACCG TGGACATCGT TAACCAGAAT 
GGAGAAACTG CCTTGGATAT AGCAAAGAGA CTCAAAGCTA CCCAGTGTGA AGATCTGCTT 
TCCCAGGCTA AATCTGGAAA GTTCAACCCT CATGTCCACG TAGAGTATGA GTGGAATCTT 
CGACAGGACG AGATGGATGA GAGCGATGAC GATCTGGATG ACAAGCCAAG CCCGATCAAA 
AAGGAACGCT CGCCCAGACC ACAGAGCTTC TGCCACTCCT CCAGCATCTC GCCACAGGAC 
AAGCTGGCAC TGCCAGGGTT CAGCACTCCA CGGGACAAGC AGCGGCTCTC CTACGGAGCC 
TTCACCAACC AGATCTTCGC CTCTACGAGC ACAGATTTGC CCACATCACC CACCAGTGAG 
GCTCCCCCTT TGCCACCTCG GAACGCCGGG AAAGGTAATG ATGTGGGCCC ATTATCTTCA 
AGTAAGACGG CCAACAAGTT TGAGGGGCTG TCTCAGCAAG CAAGCACCAG TTCTGCTAAG 
ACTGCCCTTG GCCCGAGAGT GCTTCCTAAA CTACCTCAGA AAGTGGCACT AAGGAAGACG 
GAGACCAGCC ATCATCTCTC CCTCGACAGA ACCAACATCC CACCTGAGAC TTTTCAGAAA 
TCATCACAGT TGACAGAGTT ACCCCAAAAG CCACCACTTG GAGAGCTGCC CCCGAAGCCT 
GTGGAACTGG CCCCCAAGCC CCAAGTTGGA GAGCTGCCAC CTAAGCCTGG AGAGCTACCC 
CCTAAGCCCC AATTAGGTGA CCTGCCCCCC AAGCCACAGC TCTCAGACTT ACCTCCCAAG 
CCACAGATGA AGGACCTGCC CCCTAAGCCG CAGCTGGGGG ATCTGCTGGC AAAGTCCCAG 
GCTGGCGATG TCTCAGCCAA GGTGCAGCCA CCCTCAGAGG TCACACAGAG GTCACACACC 
GGGGATCTGT CTCCAAATGT ACAGTCCAGA GATGCCATCC AGAAGCAAGC ATCTGAAGAC 
TCCAACGACC TCACACCCAC GCTGCCAGAG ACACCCGTAC CACTGCCGAG AAAAATCAAT 
ACGGGGAAAA ATAAAGTGAG GCGGGTGAAG ACCATTTATG ACTGCCAGGC AGATAATGAC 
GACGAACTCA CATTTATTGA GGGGGAAGTG ATCATTGTCA CCGGGGAAGA GGACCAGGAG 
TGGTGGATCG GGCATATCGA AGGACAGCCT GAAAGGAAGG GTGTCTTTCC AGTGTCCTTT 
GTCCACATCC TGTCTGACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asap1 ( XM_006520403.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006520466.1 Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X13, mRNA.

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CloneID	OMu50924
Clone ID Related Accession (Same CDS sequence)	XM_006520403.3 , XM_006520403.4
Accession Version	XM_006520403.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3312bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006520403.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCAG GTCTGAGTGG GCCACAGGGC TTGCCTTCCA AAGCAGGCTT TAATGGAGGC 
CAGTCATACG CTTTCAAGGG CTCCTGGAGG CCAATCCCAA GGACTAGGAT GCCGGACCAG 
ATCTCCGTGT CCGAGTTCAT CGCCGAGACC ACCGAGGACT ACAACTCGCC CACCACGTCC 
AGCTTCACCA CGCGGCTGCA CAACTGCAGG AACACCGTCA CGCTGCTGGA GGAGGCTCTA 
GACCAAGATA GAACAGCCCT ACAGAAAGTG AAGAAATCTG TAAAAGCAAT ATATAATTCC 
GGTCAAGATC ATGTACAAAA TGAAGAAAAC TATGCACAAG TTCTGGATAA GTTTGGAAGT 
AATTTTTTAA GCAGAGACAA CCCTGACCTT GGCACCGCTT TTGTCAAGTT TTCTACACTC 
ACAAAGGAAC TGTCCACGCT GCTGAAAAAT CTGCTCCAGG GTCTGAGCCA CAATGTGATC 
TTCACCTTGG ATTCCTTGTT AAAAGGAGAC TTGAAGGGGG TCAAAGGAGA TCTCAAGAAG 
CCATTTGACA AAGCCTGGAA AGATTATGAG ACGAAGTTTA CAAAAATTGA GAAGGAGAAA 
AGAGAGCACG CAAAACAGCA CGGGATGATC CGAACAGAGA TAACAGGCGC TGAGATTGCA 
GAAGAAATGG AAAAGGAACG GCGGCTTTTT CAGCTCCAGA TGTGTGAATA TCTCATTAAA 
GTAAATGAAA TCAAGACCAA AAAGGGTGTG GACCTGCTGC AGAACCTTAT AAAGTATTAC 
CATGCACAGT GCAATTTCTT TCAGGATGGC TTGAAAACGG CTGATAAACT GAAACAGTAC 
ATAGAAAAGC TGGCTGCTGA CTTATATAAT ATAAAGCAAA CCCAAGATGA AGAAAAAAAA 
CAGCTAACTG CACTCCGAGA TCTAATAAAA TCCTCTCTGC AACTTGATCC GAAAGAAGTA 
GGTGGTTTAT ATGTTGCCAG CAGGGCTAAC AGTGACTCTC AGAGCCGGCA AGGTGGGTAC 
AGCATGCATC AGCTCCAGGG CAACAAGGAG TACGGCAGTG AAAAGAAGGG CTTCCTGCTG 
AAGAAGAGTG ATGGGATCCG GAAAGTGTGG CAGAGACGGA AGTGTGCGGT CAAGAACGGG 
ATCCTGACCA TCTCCCACGC AACTTCCAAC AGGCAGCCTG CTAAGTTAAA CCTCCTCACC 
TGCCAAGTGA AACCGAATGC TGAGGACAAG AAGTCTTTTG ACCTGATATC ACATAATCGA 
ACATATCACT TTCAAGCAGA AGATGAGCAG GATTATATAG CGTGGATATC AGTACTGACG 
AATAGCAAAG AAGAGGCCCT AACCATGGCC TTCCGTGGTG AGCAGAGCAC AGGGGAGAAT 
AGCCTGGAGG ACCTTACCAA AGCCATCATC GAGGATGTAC AGCGGCTCCC TGGGAATGAC 
ATCTGCTGTG ACTGCGGCTC ATCAGAACCC ACGTGGCTTT CAACCAACTT GGGTATTTTG 
ACCTGTATAG AATGTTCCGG AATCCATAGG GAAATGGGGG TTCATATTTC TCGCATTCAG 
TCTTTGGAAC TAGACAAATT AGGAACTTCC GAACTCTTGC TGGCCAAGAA TGTAGGAAAC 
AATAGTTTTA ATGATATTAT GGAAGCAAAT TTACCCAGCC CTTCACCAAA GCCCACCCCT 
TCAAGTGATA TGACCGTGCG TAAAGAGTAC ATCACTGCAA AGTATGTCGA CCACCGCTTC 
TCCAGGAAGA CCTGTGCCTC TTCCTCAGCA AAGCTGAACG AATTGCTTGA GGCCATCAAG 
TCCAGGGACT TACTTGCATT AATCCAAGTC TATGCAGAGG GGGTGGAGTT AATGGAGCCA 
CTGCTGGAAC CTGGGCAGGA GCTCGGGGAG ACAGCCCTTC ATCTTGCCGT CAGGACCGCA 
GACCAGACAT CTCTCCATCT GGTTGATTTC CTTGTACAAA ACTGTGGGAA CCTGGATAAG 
CAAACGTCTG TGGGGAATAC AGTTCTGCAC TATTGCAGCA TGTACGGCAA GCCGGAGTGT 
CTGAAGCTGC TGCTTAGGAG CAAGCCCACC GTGGACATCG TTAACCAGAA TGGAGAAACT 
GCCTTGGATA TAGCAAAGAG ACTCAAAGCT ACCCAGTGTG AAGATCTGCT TTCCCAGGCT 
AAATCTGGAA AGTTCAACCC TCATGTCCAC GTAGAGTATG AGTGGAATCT TCGACAGGAC 
GAGATGGATG AGAGCGATGA CGATCTGGAT GACAAGCCAA GCCCGATCAA AAAGGAACGC 
TCGCCCAGAC CACAGAGCTT CTGCCACTCC TCCAGCATCT CGCCACAGGA CAAGCTGGCA 
CTGCCAGGGT TCAGCACTCC ACGGGACAAG CAGCGGCTCT CCTACGGAGC CTTCACCAAC 
CAGATCTTCG CCTCTACGAG CACAGATTTG CCCACATCAC CCACCAGTGA GGCTCCCCCT 
TTGCCACCTC GGAACGCCGG GAAAGGTAAT GATGTGGGCC CATTATCTTC AAGTAAGACG 
GCCAACAAGT TTGAGGGGCT GTCTCAGCAA GCAAGCACCA GTTCTGCTAA GACTGCCCTT 
GGCCCGAGAG TGCTTCCTAA ACTACCTCAG AAAGTGGCAC TAAGGAAGAC GGAGACCAGC 
CATCATCTCT CCCTCGACAG AACCAACATC CCACCTGAGA CTTTTCAGAA ATCATCACAG 
TTGACAGAGT TACCCCAAAA GCCACCACTT GGAGAGCTGC CCCCGAAGCC TGTGGAACTG 
GCCCCCAAGC CCCAAGTTGG AGAGCTGCCA CCTAAGCCTG GAGAGCTACC CCCTAAGCCC 
CAATTAGGTG ACCTGCCCCC CAAGCCACAG CTCTCAGACT TACCTCCCAA GCCACAGATG 
AAGGACCTGC CCCCTAAGCC GCAGCTGGGG GATCTGCTGG CAAAGTCCCA GGCTGGCGAT 
GTCTCAGCCA AGGTGCAGCC ACCCTCAGAG GTCACACAGA GGTCACACAC CGGGGATCTG 
TCTCCAAATG TACAGTCCAG AGATGCCATC CAGAAGCAAG CATCTGAAGA CTCCAACGAC 
CTCACACCCA CGCTGCCAGA GACACCCGTA CCACTGCCGA GAAAAATCAA TACGGGGAAA 
AATAAAGTGA GGCGGGTGAA GACCATTTAT GACTGCCAGG CAGATAATGA CGACGAACTC 
ACATTTATTG AGGGGGAAGT GATCATTGTC ACCGGGGAAG AGGACCAGGA GTGGTGGATC 
GGGCATATCG AAGGACAGCC TGAAAGGAAG GGTGTCTTTC CAGTGTCCTT TGTCCACATC 
CTGTCTGACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006520466.1
CDS	198..3509
Translation	MQAGLSGPQGLPSKAGFNGGQSYAFKGSWRPIPRTRMPDQISVSEFIAETTEDYNSPTTSSFTTRLHNCRNTVTLLEEALDQDRTALQKVKKSVKAIYNSGQDHVQNEENYAQVLDKFGSNFLSRDNPDLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGVKGDLKKPFDKAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDPKEVGGLYVASRANSDSQSRQGGYSMHQLQGNKEYGSEKKGFLLKKSDGIRKVWQRRKCAVKNGILTISHATSNRQPAKLNLLTCQVKPNAEDKKSFDLISHNRTYHFQAEDEQDYIAWISVLTNSKEEALTMAFRGEQSTGENSLEDLTKAIIEDVQRLPGNDICCDCGSSEPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDKLGTSELLLAKNVGNNSFNDIMEANLPSPSPKPTPSSDMTVRKEYITAKYVDHRFSRKTCASSSAKLNELLEAIKSRDLLALIQVYAEGVELMEPLLEPGQELGETALHLAVRTADQTSLHLVDFLVQNCGNLDKQTSVGNTVLHYCSMYGKPECLKLLLRSKPTVDIVNQNGETALDIAKRLKATQCEDLLSQAKSGKFNPHVHVEYEWNLRQDEMDESDDDLDDKPSPIKKERSPRPQSFCHSSSISPQDKLALPGFSTPRDKQRLSYGAFTNQIFASTSTDLPTSPTSEAPPLPPRNAGKGNDVGPLSSSKTANKFEGLSQQASTSSAKTALGPRVLPKLPQKVALRKTETSHHLSLDRTNIPPETFQKSSQLTELPQKPPLGELPPKPVELAPKPQVGELPPKPGELPPKPQLGDLPPKPQLSDLPPKPQMKDLPPKPQLGDLLAKSQAGDVSAKVQPPSEVTQRSHTGDLSPNVQSRDAIQKQASEDSNDLTPTLPETPVPLPRKINTGKNKVRRVKTIYDCQADNDDELTFIEGEVIIVTGEEDQEWWIGHIEGQPERKGVFPVSFVHILSD

Target ORF information:

RefSeq Version	XM_006520403.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006520403.3

ORF Insert Sequence:

ATGCAGGCAG GTCTGAGTGG GCCACAGGGC TTGCCTTCCA AAGCAGGCTT TAATGGAGGC 
CAGTCATACG CTTTCAAGGG CTCCTGGAGG CCAATCCCAA GGACTAGGAT GCCGGACCAG 
ATCTCCGTGT CCGAGTTCAT CGCCGAGACC ACCGAGGACT ACAACTCGCC CACCACGTCC 
AGCTTCACCA CGCGGCTGCA CAACTGCAGG AACACCGTCA CGCTGCTGGA GGAGGCTCTA 
GACCAAGATA GAACAGCCCT ACAGAAAGTG AAGAAATCTG TAAAAGCAAT ATATAATTCC 
GGTCAAGATC ATGTACAAAA TGAAGAAAAC TATGCACAAG TTCTGGATAA GTTTGGAAGT 
AATTTTTTAA GCAGAGACAA CCCTGACCTT GGCACCGCTT TTGTCAAGTT TTCTACACTC 
ACAAAGGAAC TGTCCACGCT GCTGAAAAAT CTGCTCCAGG GTCTGAGCCA CAATGTGATC 
TTCACCTTGG ATTCCTTGTT AAAAGGAGAC TTGAAGGGGG TCAAAGGAGA TCTCAAGAAG 
CCATTTGACA AAGCCTGGAA AGATTATGAG ACGAAGTTTA CAAAAATTGA GAAGGAGAAA 
AGAGAGCACG CAAAACAGCA CGGGATGATC CGAACAGAGA TAACAGGCGC TGAGATTGCA 
GAAGAAATGG AAAAGGAACG GCGGCTTTTT CAGCTCCAGA TGTGTGAATA TCTCATTAAA 
GTAAATGAAA TCAAGACCAA AAAGGGTGTG GACCTGCTGC AGAACCTTAT AAAGTATTAC 
CATGCACAGT GCAATTTCTT TCAGGATGGC TTGAAAACGG CTGATAAACT GAAACAGTAC 
ATAGAAAAGC TGGCTGCTGA CTTATATAAT ATAAAGCAAA CCCAAGATGA AGAAAAAAAA 
CAGCTAACTG CACTCCGAGA TCTAATAAAA TCCTCTCTGC AACTTGATCC GAAAGAAGTA 
GGTGGTTTAT ATGTTGCCAG CAGGGCTAAC AGTGACTCTC AGAGCCGGCA AGGTGGGTAC 
AGCATGCATC AGCTCCAGGG CAACAAGGAG TACGGCAGTG AAAAGAAGGG CTTCCTGCTG 
AAGAAGAGTG ATGGGATCCG GAAAGTGTGG CAGAGACGGA AGTGTGCGGT CAAGAACGGG 
ATCCTGACCA TCTCCCACGC AACTTCCAAC AGGCAGCCTG CTAAGTTAAA CCTCCTCACC 
TGCCAAGTGA AACCGAATGC TGAGGACAAG AAGTCTTTTG ACCTGATATC ACATAATCGA 
ACATATCACT TTCAAGCAGA AGATGAGCAG GATTATATAG CGTGGATATC AGTACTGACG 
AATAGCAAAG AAGAGGCCCT AACCATGGCC TTCCGTGGTG AGCAGAGCAC AGGGGAGAAT 
AGCCTGGAGG ACCTTACCAA AGCCATCATC GAGGATGTAC AGCGGCTCCC TGGGAATGAC 
ATCTGCTGTG ACTGCGGCTC ATCAGAACCC ACGTGGCTTT CAACCAACTT GGGTATTTTG 
ACCTGTATAG AATGTTCCGG AATCCATAGG GAAATGGGGG TTCATATTTC TCGCATTCAG 
TCTTTGGAAC TAGACAAATT AGGAACTTCC GAACTCTTGC TGGCCAAGAA TGTAGGAAAC 
AATAGTTTTA ATGATATTAT GGAAGCAAAT TTACCCAGCC CTTCACCAAA GCCCACCCCT 
TCAAGTGATA TGACCGTGCG TAAAGAGTAC ATCACTGCAA AGTATGTCGA CCACCGCTTC 
TCCAGGAAGA CCTGTGCCTC TTCCTCAGCA AAGCTGAACG AATTGCTTGA GGCCATCAAG 
TCCAGGGACT TACTTGCATT AATCCAAGTC TATGCAGAGG GGGTGGAGTT AATGGAGCCA 
CTGCTGGAAC CTGGGCAGGA GCTCGGGGAG ACAGCCCTTC ATCTTGCCGT CAGGACCGCA 
GACCAGACAT CTCTCCATCT GGTTGATTTC CTTGTACAAA ACTGTGGGAA CCTGGATAAG 
CAAACGTCTG TGGGGAATAC AGTTCTGCAC TATTGCAGCA TGTACGGCAA GCCGGAGTGT 
CTGAAGCTGC TGCTTAGGAG CAAGCCCACC GTGGACATCG TTAACCAGAA TGGAGAAACT 
GCCTTGGATA TAGCAAAGAG ACTCAAAGCT ACCCAGTGTG AAGATCTGCT TTCCCAGGCT 
AAATCTGGAA AGTTCAACCC TCATGTCCAC GTAGAGTATG AGTGGAATCT TCGACAGGAC 
GAGATGGATG AGAGCGATGA CGATCTGGAT GACAAGCCAA GCCCGATCAA AAAGGAACGC 
TCGCCCAGAC CACAGAGCTT CTGCCACTCC TCCAGCATCT CGCCACAGGA CAAGCTGGCA 
CTGCCAGGGT TCAGCACTCC ACGGGACAAG CAGCGGCTCT CCTACGGAGC CTTCACCAAC 
CAGATCTTCG CCTCTACGAG CACAGATTTG CCCACATCAC CCACCAGTGA GGCTCCCCCT 
TTGCCACCTC GGAACGCCGG GAAAGGTAAT GATGTGGGCC CATTATCTTC AAGTAAGACG 
GCCAACAAGT TTGAGGGGCT GTCTCAGCAA GCAAGCACCA GTTCTGCTAA GACTGCCCTT 
GGCCCGAGAG TGCTTCCTAA ACTACCTCAG AAAGTGGCAC TAAGGAAGAC GGAGACCAGC 
CATCATCTCT CCCTCGACAG AACCAACATC CCACCTGAGA CTTTTCAGAA ATCATCACAG 
TTGACAGAGT TACCCCAAAA GCCACCACTT GGAGAGCTGC CCCCGAAGCC TGTGGAACTG 
GCCCCCAAGC CCCAAGTTGG AGAGCTGCCA CCTAAGCCTG GAGAGCTACC CCCTAAGCCC 
CAATTAGGTG ACCTGCCCCC CAAGCCACAG CTCTCAGACT TACCTCCCAA GCCACAGATG 
AAGGACCTGC CCCCTAAGCC GCAGCTGGGG GATCTGCTGG CAAAGTCCCA GGCTGGCGAT 
GTCTCAGCCA AGGTGCAGCC ACCCTCAGAG GTCACACAGA GGTCACACAC CGGGGATCTG 
TCTCCAAATG TACAGTCCAG AGATGCCATC CAGAAGCAAG CATCTGAAGA CTCCAACGAC 
CTCACACCCA CGCTGCCAGA GACACCCGTA CCACTGCCGA GAAAAATCAA TACGGGGAAA 
AATAAAGTGA GGCGGGTGAA GACCATTTAT GACTGCCAGG CAGATAATGA CGACGAACTC 
ACATTTATTG AGGGGGAAGT GATCATTGTC ACCGGGGAAG AGGACCAGGA GTGGTGGATC 
GGGCATATCG AAGGACAGCC TGAAAGGAAG GGTGTCTTTC CAGTGTCCTT TGTCCACATC 
CTGTCTGACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asap1 ( XM_011245448.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011243750.1 Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OMu67522
Clone ID Related Accession (Same CDS sequence)	XM_011245448.1
Accession Version	XM_011245448.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3291bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGCCA TTCAACCCAG AGCCTCACGC ATGGCTCTAG ACCAAGATAG AACAGCCCTA 
CAGAAAGTGA AGAAATCTGT AAAAGCAATA TATAATTCCG GTCAAGATCA TGTACAAAAT 
GAAGAAAACT ATGCACAAGT TCTGGATAAG TTTGGAAGTA ATTTTTTAAG CAGAGACAAC 
CCTGACCTTG GCACCGCTTT TGTCAAGTTT TCTACACTCA CAAAGGAACT GTCCACGCTG 
CTGAAAAATC TGCTCCAGGG TCTGAGCCAC AATGTGATCT TCACCTTGGA TTCCTTGTTA 
AAAGGAGACT TGAAGGGGGT CAAAGGAGAT CTCAAGAAGC CATTTGACAA AGCCTGGAAA 
GATTATGAGA CGAAGTTTAC AAAAATTGAG AAGGAGAAAA GAGAGCACGC AAAACAGCAC 
GGGATGATCC GAACAGAGAT AACAGGCGCT GAGATTGCAG AAGAAATGGA AAAGGAACGG 
CGGCTTTTTC AGCTCCAGAT GTGTGAATAT CTCATTAAAG TAAATGAAAT CAAGACCAAA 
AAGGGTGTGG ACCTGCTGCA GAACCTTATA AAGTATTACC ATGCACAGTG CAATTTCTTT 
CAGGATGGCT TGAAAACGGC TGATAAACTG AAACAGTACA TAGAAAAGCT GGCTGCTGAC 
TTATATAATA TAAAGCAAAC CCAAGATGAA GAAAAAAAAC AGCTAACTGC ACTCCGAGAT 
CTAATAAAAT CCTCTCTGCA ACTTGATCCG AAAGAAGTAG GTGGTTTATA TGTTGCCAGC 
AGGGCTAACA GTTCTAGAAG AGACTCTCAG AGCCGGCAAG GTGGGTACAG CATGCATCAG 
CTCCAGGGCA ACAAGGAGTA CGGCAGTGAA AAGAAGGGCT TCCTGCTGAA GAAGAGTGAT 
GGGATCCGGA AAGTGTGGCA GAGACGGAAG TGTGCGGTCA AGAACGGGAT CCTGACCATC 
TCCCACGCAA CTTCCAACAG GCAGCCTGCT AAGTTAAACC TCCTCACCTG CCAAGTGAAA 
CCGAATGCTG AGGACAAGAA GTCTTTTGAC CTGATATCAC ATAATCGAAC ATATCACTTT 
CAAGCAGAAG ATGAGCAGGA TTATATAGCG TGGATATCAG TACTGACGAA TAGCAAAGAA 
GAGGCCCTAA CCATGGCCTT CCGTGGTGAG CAGAGCACAG GGGAGAATAG CCTGGAGGAC 
CTTACCAAAG CCATCATCGA GGATGTACAG CGGCTCCCTG GGAATGACAT CTGCTGTGAC 
TGCGGCTCAT CAGAACCCAC GTGGCTTTCA ACCAACTTGG GTATTTTGAC CTGTATAGAA 
TGTTCCGGAA TCCATAGGGA AATGGGGGTT CATATTTCTC GCATTCAGTC TTTGGAACTA 
GACAAATTAG GAACTTCCGA ACTCTTGCTG GCCAAGAATG TAGGAAACAA TAGTTTTAAT 
GATATTATGG AAGCAAATTT ACCCAGCCCT TCACCAAAGC CCACCCCTTC AAGTGATATG 
ACCGTGCGTA AAGAGTACAT CACTGCAAAG TATGTCGACC ACCGCTTCTC CAGGAAGACC 
TGTGCCTCTT CCTCAGCAAA GCTGAACGAA TTGCTTGAGG CCATCAAGTC CAGGGACTTA 
CTTGCATTAA TCCAAGTCTA TGCAGAGGGG GTGGAGTTAA TGGAGCCACT GCTGGAACCT 
GGGCAGGAGC TCGGGGAGAC AGCCCTTCAT CTTGCCGTCA GGACCGCAGA CCAGACATCT 
CTCCATCTGG TTGATTTCCT TGTACAAAAC TGTGGGAACC TGGATAAGCA AACGTCTGTG 
GGGAATACAG TTCTGCACTA TTGCAGCATG TACGGCAAGC CGGAGTGTCT GAAGCTGCTG 
CTTAGGAGCA AGCCCACCGT GGACATCGTT AACCAGAATG GAGAAACTGC CTTGGATATA 
GCAAAGAGAC TCAAAGCTAC CCAGTGTGAA GATCTGCTTT CCCAGGCTAA ATCTGGAAAG 
TTCAACCCTC ATGTCCACGT AGAGTATGAG TGGAATCTTC GACAGGACGA GATGGATGAG 
AGCGATGACG ATCTGGATGA CAAGCCAAGC CCGATCAAAA AGGAACGCTC GCCCAGACCA 
CAGAGCTTCT GCCACTCCTC CAGCATCTCG CCACAGGACA AGCTGGCACT GCCAGGGTTC 
AGCACTCCAC GGGACAAGCA GCGGCTCTCC TACGGAGCCT TCACCAACCA GATCTTCGCC 
TCTACGAGCA CAGATTTGCC CACATCACCC ACCAGTGAGG CTCCCCCTTT GCCACCTCGG 
AACGCCGGGA AAGGTCCAAC TGGCCCACCT TCAACACTCC CTCTAGGCAC CCAGACCTCT 
AGTGGCAGCT CCACCCTATC CAAGAAGAGG CCTCCTCCCC CACCACCAGG ACACAAGAGA 
ACCCTGTCTG ACCCTCCCAG CCCACTACCT CACGGGCCCC CAAACAAAGG CGCAATTCCT 
TGGGGTAATG ATGTGGGCCC ATTATCTTCA AGTAAGACGG CCAACAAGTT TGAGGGGCTG 
TCTCAGCAAG CAAGCACCAG TTCTGCTAAG ACTGCCCTTG GCCCGAGAGT GCTTCCTAAA 
CTACCTCAGA AAGTGGCACT AAGGAAGACG GAGACCAGCC ATCATCTCTC CCTCGACAGA 
ACCAACATCC CACCTGAGAC TTTTCAGAAA TCATCACAGT TGACAGAGTT ACCCCAAAAG 
CCACCACTTG GAGAGCTGCC CCCGAAGCCT GTGGAACTGG CCCCCAAGCC CCAAGTTGGA 
GAGCTGCCAC CTAAGCCTGG AGAGCTACCC CCTAAGCCCC AATTAGGTGA CCTGCCCCCC 
AAGCCACAGC TCTCAGACTT ACCTCCCAAG CCACAGATGA AGGACCTGCC CCCTAAGCCG 
CAGCTGGGGG ATCTGCTGGC AAAGTCCCAG GCTGGCGATG TCTCAGCCAA GGTGCAGCCA 
CCCTCAGAGG TCACACAGAG GTCACACACC GGGGATCTGT CTCCAAATGT ACAGTCCAGA 
GATGCCATCC AGAAGCAAGC ATCTGAAGAC TCCAACGACC TCACACCCAC GCTGCCAGAG 
ACACCCGTAC CACTGCCGAG AAAAATCAAT ACGGGGAAAA ATAAAGTGAG GCGGGTGAAG 
ACCATTTATG ACTGCCAGGC AGATAATGAC GACGAACTCA CATTTATTGA GGGGGAAGTG 
ATCATTGTCA CCGGGGAAGA GGACCAGGAG TGGTGGATCG GGCATATCGA AGGACAGCCT 
GAAAGGAAGG GTGTCTTTCC AGTGTCCTTT GTCCACATCC TGTCTGACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011243750.1
CDS	122..3412
Translation	MLAIQPRASRMALDQDRTALQKVKKSVKAIYNSGQDHVQNEENYAQVLDKFGSNFLSRDNPDLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGVKGDLKKPFDKAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDPKEVGGLYVASRANSSRRDSQSRQGGYSMHQLQGNKEYGSEKKGFLLKKSDGIRKVWQRRKCAVKNGILTISHATSNRQPAKLNLLTCQVKPNAEDKKSFDLISHNRTYHFQAEDEQDYIAWISVLTNSKEEALTMAFRGEQSTGENSLEDLTKAIIEDVQRLPGNDICCDCGSSEPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDKLGTSELLLAKNVGNNSFNDIMEANLPSPSPKPTPSSDMTVRKEYITAKYVDHRFSRKTCASSSAKLNELLEAIKSRDLLALIQVYAEGVELMEPLLEPGQELGETALHLAVRTADQTSLHLVDFLVQNCGNLDKQTSVGNTVLHYCSMYGKPECLKLLLRSKPTVDIVNQNGETALDIAKRLKATQCEDLLSQAKSGKFNPHVHVEYEWNLRQDEMDESDDDLDDKPSPIKKERSPRPQSFCHSSSISPQDKLALPGFSTPRDKQRLSYGAFTNQIFASTSTDLPTSPTSEAPPLPPRNAGKGPTGPPSTLPLGTQTSSGSSTLSKKRPPPPPPGHKRTLSDPPSPLPHGPPNKGAIPWGNDVGPLSSSKTANKFEGLSQQASTSSAKTALGPRVLPKLPQKVALRKTETSHHLSLDRTNIPPETFQKSSQLTELPQKPPLGELPPKPVELAPKPQVGELPPKPGELPPKPQLGDLPPKPQLSDLPPKPQMKDLPPKPQLGDLLAKSQAGDVSAKVQPPSEVTQRSHTGDLSPNVQSRDAIQKQASEDSNDLTPTLPETPVPLPRKINTGKNKVRRVKTIYDCQADNDDELTFIEGEVIIVTGEEDQEWWIGHIEGQPERKGVFPVSFVHILSD

Target ORF information:

RefSeq Version	XM_011245448.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011245448.1

ORF Insert Sequence:

ATGTTGGCCA TTCAACCCAG AGCCTCACGC ATGGCTCTAG ACCAAGATAG AACAGCCCTA 
CAGAAAGTGA AGAAATCTGT AAAAGCAATA TATAATTCCG GTCAAGATCA TGTACAAAAT 
GAAGAAAACT ATGCACAAGT TCTGGATAAG TTTGGAAGTA ATTTTTTAAG CAGAGACAAC 
CCTGACCTTG GCACCGCTTT TGTCAAGTTT TCTACACTCA CAAAGGAACT GTCCACGCTG 
CTGAAAAATC TGCTCCAGGG TCTGAGCCAC AATGTGATCT TCACCTTGGA TTCCTTGTTA 
AAAGGAGACT TGAAGGGGGT CAAAGGAGAT CTCAAGAAGC CATTTGACAA AGCCTGGAAA 
GATTATGAGA CGAAGTTTAC AAAAATTGAG AAGGAGAAAA GAGAGCACGC AAAACAGCAC 
GGGATGATCC GAACAGAGAT AACAGGCGCT GAGATTGCAG AAGAAATGGA AAAGGAACGG 
CGGCTTTTTC AGCTCCAGAT GTGTGAATAT CTCATTAAAG TAAATGAAAT CAAGACCAAA 
AAGGGTGTGG ACCTGCTGCA GAACCTTATA AAGTATTACC ATGCACAGTG CAATTTCTTT 
CAGGATGGCT TGAAAACGGC TGATAAACTG AAACAGTACA TAGAAAAGCT GGCTGCTGAC 
TTATATAATA TAAAGCAAAC CCAAGATGAA GAAAAAAAAC AGCTAACTGC ACTCCGAGAT 
CTAATAAAAT CCTCTCTGCA ACTTGATCCG AAAGAAGTAG GTGGTTTATA TGTTGCCAGC 
AGGGCTAACA GTTCTAGAAG AGACTCTCAG AGCCGGCAAG GTGGGTACAG CATGCATCAG 
CTCCAGGGCA ACAAGGAGTA CGGCAGTGAA AAGAAGGGCT TCCTGCTGAA GAAGAGTGAT 
GGGATCCGGA AAGTGTGGCA GAGACGGAAG TGTGCGGTCA AGAACGGGAT CCTGACCATC 
TCCCACGCAA CTTCCAACAG GCAGCCTGCT AAGTTAAACC TCCTCACCTG CCAAGTGAAA 
CCGAATGCTG AGGACAAGAA GTCTTTTGAC CTGATATCAC ATAATCGAAC ATATCACTTT 
CAAGCAGAAG ATGAGCAGGA TTATATAGCG TGGATATCAG TACTGACGAA TAGCAAAGAA 
GAGGCCCTAA CCATGGCCTT CCGTGGTGAG CAGAGCACAG GGGAGAATAG CCTGGAGGAC 
CTTACCAAAG CCATCATCGA GGATGTACAG CGGCTCCCTG GGAATGACAT CTGCTGTGAC 
TGCGGCTCAT CAGAACCCAC GTGGCTTTCA ACCAACTTGG GTATTTTGAC CTGTATAGAA 
TGTTCCGGAA TCCATAGGGA AATGGGGGTT CATATTTCTC GCATTCAGTC TTTGGAACTA 
GACAAATTAG GAACTTCCGA ACTCTTGCTG GCCAAGAATG TAGGAAACAA TAGTTTTAAT 
GATATTATGG AAGCAAATTT ACCCAGCCCT TCACCAAAGC CCACCCCTTC AAGTGATATG 
ACCGTGCGTA AAGAGTACAT CACTGCAAAG TATGTCGACC ACCGCTTCTC CAGGAAGACC 
TGTGCCTCTT CCTCAGCAAA GCTGAACGAA TTGCTTGAGG CCATCAAGTC CAGGGACTTA 
CTTGCATTAA TCCAAGTCTA TGCAGAGGGG GTGGAGTTAA TGGAGCCACT GCTGGAACCT 
GGGCAGGAGC TCGGGGAGAC AGCCCTTCAT CTTGCCGTCA GGACCGCAGA CCAGACATCT 
CTCCATCTGG TTGATTTCCT TGTACAAAAC TGTGGGAACC TGGATAAGCA AACGTCTGTG 
GGGAATACAG TTCTGCACTA TTGCAGCATG TACGGCAAGC CGGAGTGTCT GAAGCTGCTG 
CTTAGGAGCA AGCCCACCGT GGACATCGTT AACCAGAATG GAGAAACTGC CTTGGATATA 
GCAAAGAGAC TCAAAGCTAC CCAGTGTGAA GATCTGCTTT CCCAGGCTAA ATCTGGAAAG 
TTCAACCCTC ATGTCCACGT AGAGTATGAG TGGAATCTTC GACAGGACGA GATGGATGAG 
AGCGATGACG ATCTGGATGA CAAGCCAAGC CCGATCAAAA AGGAACGCTC GCCCAGACCA 
CAGAGCTTCT GCCACTCCTC CAGCATCTCG CCACAGGACA AGCTGGCACT GCCAGGGTTC 
AGCACTCCAC GGGACAAGCA GCGGCTCTCC TACGGAGCCT TCACCAACCA GATCTTCGCC 
TCTACGAGCA CAGATTTGCC CACATCACCC ACCAGTGAGG CTCCCCCTTT GCCACCTCGG 
AACGCCGGGA AAGGTCCAAC TGGCCCACCT TCAACACTCC CTCTAGGCAC CCAGACCTCT 
AGTGGCAGCT CCACCCTATC CAAGAAGAGG CCTCCTCCCC CACCACCAGG ACACAAGAGA 
ACCCTGTCTG ACCCTCCCAG CCCACTACCT CACGGGCCCC CAAACAAAGG CGCAATTCCT 
TGGGGTAATG ATGTGGGCCC ATTATCTTCA AGTAAGACGG CCAACAAGTT TGAGGGGCTG 
TCTCAGCAAG CAAGCACCAG TTCTGCTAAG ACTGCCCTTG GCCCGAGAGT GCTTCCTAAA 
CTACCTCAGA AAGTGGCACT AAGGAAGACG GAGACCAGCC ATCATCTCTC CCTCGACAGA 
ACCAACATCC CACCTGAGAC TTTTCAGAAA TCATCACAGT TGACAGAGTT ACCCCAAAAG 
CCACCACTTG GAGAGCTGCC CCCGAAGCCT GTGGAACTGG CCCCCAAGCC CCAAGTTGGA 
GAGCTGCCAC CTAAGCCTGG AGAGCTACCC CCTAAGCCCC AATTAGGTGA CCTGCCCCCC 
AAGCCACAGC TCTCAGACTT ACCTCCCAAG CCACAGATGA AGGACCTGCC CCCTAAGCCG 
CAGCTGGGGG ATCTGCTGGC AAAGTCCCAG GCTGGCGATG TCTCAGCCAA GGTGCAGCCA 
CCCTCAGAGG TCACACAGAG GTCACACACC GGGGATCTGT CTCCAAATGT ACAGTCCAGA 
GATGCCATCC AGAAGCAAGC ATCTGAAGAC TCCAACGACC TCACACCCAC GCTGCCAGAG 
ACACCCGTAC CACTGCCGAG AAAAATCAAT ACGGGGAAAA ATAAAGTGAG GCGGGTGAAG 
ACCATTTATG ACTGCCAGGC AGATAATGAC GACGAACTCA CATTTATTGA GGGGGAAGTG 
ATCATTGTCA CCGGGGAAGA GGACCAGGAG TGGTGGATCG GGCATATCGA AGGACAGCCT 
GAAAGGAAGG GTGTCTTTCC AGTGTCCTTT GTCCACATCC TGTCTGACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asap1 ( XM_017316425.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171914.1 Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OMu78998
Clone ID Related Accession (Same CDS sequence)	XM_017316425.1
Accession Version	XM_017316425.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAGAT GCATGCTTTG CATTCCTTTT GAATTCCCTT TGAGTCCCCA GGTCAGGCTC 
CAACACCATG TAAGGGATGG GCTTTCGACT GTGCTCATCC TGCCTTTCAT GTCTGCTCTG 
CTTCCCCAGC GTGAAGCTAG TGCCGAAGAG CTCCGCCAGC TTTATGAGTG GAGCAGGAAC 
CCACACTCAT TAAACACAAC AGGATGCCGG ACCAGATCTC CGTGTCCGAG TTCATCGCCG 
AGACCACCGA GGACTACAAC TCGCCCACCA CGTCCAGCTT CACCACGCGG CTGCACAACT 
GCAGGAACAC CGTCACGCTG CTGGAGGAGC GTGGTTGACG GTGGCAGCCG AGCAGTGGTG 
CAGATGACGC CAGCTGTGTT GCACTTGGGT GAGCCATACA CAAGTTCATT TGAGGCTCTA 
GACCAAGATA GAACAGCCCT ACAGAAAGTG AAGAAATCTG TAAAAGCAAT ATATAATTCC 
GGTCAAGATC ATGTACAAAA TGAAGAAAAC TATGCACAAG TTCTGGATAA GTTTGGAAGT 
AATTTTTTAA GCAGAGACAA CCCTGACCTT GGCACCGCTT TTGTCAAGTT TTCTACACTC 
ACAAAGGAAC TGTCCACGCT GCTGAAAAAT CTGCTCCAGG GTCTGAGCCA CAATGTGATC 
TTCACCTTGG ATTCCTTGTT AAAAGGAGAC TTGAAGGGGG TCAAAGGAGA TCTCAAGAAG 
CCATTTGACA AAGCCTGGAA AGATTATGAG ACGAAGTTTA CAAAAATTGA GAAGGAGAAA 
AGAGAGCACG CAAAACAGCA CGGGATGATC CGAACAGAGA TAACAGGCGC TGAGATTGCA 
GAAGAAATGG AAAAGGAACG GCGGCTTTTT CAGCTCCAGA TGTGTGAATA TCTCATTAAA 
GTAAATGAAA TCAAGACCAA AAAGGGTGTG GACCTGCTGC AGAACCTTAT AAAGTATTAC 
CATGCACAGT GCAATTTCTT TCAGGATGGC TTGAAAACGG CTGATAAACT GAAACAGTAC 
ATAGAAAAGC TGGCTGCTGA CTTATATAAT ATAAAGCAAA CCCAAGATGA AGAAAAAAAA 
CAGCTAACTG CACTCCGAGA TCTAATAAAA TCCTCTCTGC AACTTGATCC GAAAGAATCT 
AGAAGAGACT CTCAGAGCCG GCAAGGTGGG TACAGCATGC ATCAGCTCCA GGGCAACAAG 
GAGTACGGCA GTGAAAAGAA GGGCTTCCTG CTGAAGAAGA GTGATGGGAT CCGGAAAGTG 
TGGCAGAGAC GGAAGTGTGC GGTCAAGAAC GGGATCCTGA CCATCTCCCA CGCAACTTCC 
AACAGGCAGC CTGCTAAGTT AAACCTCCTC ACCTGCCAAG TGAAACCGAA TGCTGAGGAC 
AAGAAGTCTT TTGACCTGAT ATCACATAAT CGAACATATC ACTTTCAAGC AGAAGATGAG 
CAGGATTATA TAGCGTGGAT ATCAGTACTG ACGAATAGCA AAGAAGAGGC CCTAACCATG 
GCCTTCCGTG GTGAGCAGAG CACAGGGGAG AATAGCCTGG AGGACCTTAC CAAAGCCATC 
ATCGAGGATG TACAGCGGCT CCCTGGGAAT GACATCTGCT GTGACTGCGG CTCATCAGAA 
CCCACGTGGC TTTCAACCAA CTTGGGTATT TTGACCTGTA TAGAATGTTC CGGAATCCAT 
AGGGAAATGG GGGTTCATAT TTCTCGCATT CAGTCTTTGG AACTAGACAA ATTAGGAACT 
TCCGAACTCT TGCTGGCCAA GAATGTAGGA AACAATAGTT TTAATGATAT TATGGAAGCA 
AATTTACCCA GCCCTTCACC AAAGCCCACC CCTTCAAGTG ATATGACCGT GCGTAAAGAG 
TACATCACTG CAAAGTATGT CGACCACCGC TTCTCCAGGA AGACCTGTGC CTCTTCCTCA 
GCAAAGCTGA ACGAATTGCT TGAGGCCATC AAGTCCAGGG ACTTACTTGC ATTAATCCAA 
GTCTATGCAG AGGGGGTGGA GTTAATGGAG CCACTGCTGG AACCTGGGCA GGAGCTCGGG 
GAGACAGCCC TTCATCTTGC CGTCAGGACC GCAGACCAGA CATCTCTCCA TCTGGTTGAT 
TTCCTTGTAC AAAACTGTGG GAACCTGGAT AAGCAAACGT CTGTGGGGAA TACAGTTCTG 
CACTATTGCA GCATGTACGG CAAGCCGGAG TGTCTGAAGC TGCTGCTTAG GAGCAAGCCC 
ACCGTGGACA TCGTTAACCA GAATGGAGAA ACTGCCTTGG ATATAGCAAA GAGACTCAAA 
GCTACCCAGT GTGAAGATCT GCTTTCCCAG GCTAAATCTG GAAAGTTCAA CCCTCATGTC 
CACGTAGAGT ATGAGTGGAA TCTTCGACAG GACGAGATGG ATGAGAGCGA TGACGATCTG 
GATGACAAGC CAAGCCCGAT CAAAAAGGAA CGCTCGCCCA GACCACAGAG CTTCTGCCAC 
TCCTCCAGCA TCTCGCCACA GGACAAGCTG GCACTGCCAG GGTTCAGCAC TCCACGGGAC 
AAGCAGCGGC TCTCCTACGG AGCCTTCACC AACCAGATCT TCGCCTCTAC GAGCACAGAT 
TTGCCCACAT CACCCACCAG TGAGGCTCCC CCTTTGCCAC CTCGGAACGC CGGGAAAGGT 
AATGATGTGG GCCCATTATC TTCAAGTAAG ACGGCCAACA AGTTTGAGGG GCTGTCTCAG 
CAAGCAAGCA CCAGTTCTGC TAAGACTGCC CTTGGCCCGA GAGTGCTTCC TAAACTACCT 
CAGAAAGTGG CACTAAGGAA GACGGAGACC AGCCATCATC TCTCCCTCGA CAGAACCAAC 
ATCCCACCTG AGACTTTTCA GAAATCATCA CAGTTGACAG AGTTACCCCA AAAGCCACCA 
CTTGGAGAGC TGCCCCCGAA GCCTGTGGAA CTGGCCCCCA AGCCCCAAGT TGGAGAGCTG 
CCACCTAAGC CTGGAGAGCT ACCCCCTAAG CCCCAATTAG GTGACCTGCC CCCCAAGCCA 
CAGCTCTCAG ACTTACCTCC CAAGCCACAG ATGAAGGACC TGCCCCCTAA GCCGCAGCTG 
GGGGATCTGC TGGCAAAGTC CCAGGCTGGC GATGTCTCAG CCAAGGTGCA GCCACCCTCA 
GAGGTCACAC AGAGGTCACA CACCGGGGAT CTGTCTCCAA ATGTACAGTC CAGAGATGCC 
ATCCAGAAGC AAGCATCTGA AGACTCCAAC GACCTCACAC CCACGCTGCC AGAGACACCC 
GTACCACTGC CGAGAAAAAT CAATACGGGG AAAAATAAAG TGAGGCGGGT GAAGACCATT 
TATGACTGCC AGGCAGATAA TGACGACGAA CTCACATTTA TTGAGGGGGA AGTGATCATT 
GTCACCGGGG AAGAGGACCA GGAGTGGTGG ATCGGGCATA TCGAAGGACA GCCTGAAAGG 
AAGGGTGTCT TTCCAGTGTC CTTTGTCCAC ATCCTGTCTG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171914.1
CDS	16..3480
Translation	MSRCMLCIPFEFPLSPQVRLQHHVRDGLSTVLILPFMSALLPQREASAEELRQLYEWSRNPHSLNTTGCRTRSPCPSSSPRPPRTTTRPPRPASPRGCTTAGTPSRCWRSVVDGGSRAVVQMTPAVLHLGEPYTSSFEALDQDRTALQKVKKSVKAIYNSGQDHVQNEENYAQVLDKFGSNFLSRDNPDLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGVKGDLKKPFDKAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDPKESRRDSQSRQGGYSMHQLQGNKEYGSEKKGFLLKKSDGIRKVWQRRKCAVKNGILTISHATSNRQPAKLNLLTCQVKPNAEDKKSFDLISHNRTYHFQAEDEQDYIAWISVLTNSKEEALTMAFRGEQSTGENSLEDLTKAIIEDVQRLPGNDICCDCGSSEPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDKLGTSELLLAKNVGNNSFNDIMEANLPSPSPKPTPSSDMTVRKEYITAKYVDHRFSRKTCASSSAKLNELLEAIKSRDLLALIQVYAEGVELMEPLLEPGQELGETALHLAVRTADQTSLHLVDFLVQNCGNLDKQTSVGNTVLHYCSMYGKPECLKLLLRSKPTVDIVNQNGETALDIAKRLKATQCEDLLSQAKSGKFNPHVHVEYEWNLRQDEMDESDDDLDDKPSPIKKERSPRPQSFCHSSSISPQDKLALPGFSTPRDKQRLSYGAFTNQIFASTSTDLPTSPTSEAPPLPPRNAGKGNDVGPLSSSKTANKFEGLSQQASTSSAKTALGPRVLPKLPQKVALRKTETSHHLSLDRTNIPPETFQKSSQLTELPQKPPLGELPPKPVELAPKPQVGELPPKPGELPPKPQLGDLPPKPQLSDLPPKPQMKDLPPKPQLGDLLAKSQAGDVSAKVQPPSEVTQRSHTGDLSPNVQSRDAIQKQASEDSNDLTPTLPETPVPLPRKINTGKNKVRRVKTIYDCQADNDDELTFIEGEVIIVTGEEDQEWWIGHIEGQPERKGVFPVSFVHILSD

Target ORF information:

RefSeq Version	XM_017316425.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316425.1

ORF Insert Sequence:

ATGTCGAGAT GCATGCTTTG CATTCCTTTT GAATTCCCTT TGAGTCCCCA GGTCAGGCTC 
CAACACCATG TAAGGGATGG GCTTTCGACT GTGCTCATCC TGCCTTTCAT GTCTGCTCTG 
CTTCCCCAGC GTGAAGCTAG TGCCGAAGAG CTCCGCCAGC TTTATGAGTG GAGCAGGAAC 
CCACACTCAT TAAACACAAC AGGATGCCGG ACCAGATCTC CGTGTCCGAG TTCATCGCCG 
AGACCACCGA GGACTACAAC TCGCCCACCA CGTCCAGCTT CACCACGCGG CTGCACAACT 
GCAGGAACAC CGTCACGCTG CTGGAGGAGC GTGGTTGACG GTGGCAGCCG AGCAGTGGTG 
CAGATGACGC CAGCTGTGTT GCACTTGGGT GAGCCATACA CAAGTTCATT TGAGGCTCTA 
GACCAAGATA GAACAGCCCT ACAGAAAGTG AAGAAATCTG TAAAAGCAAT ATATAATTCC 
GGTCAAGATC ATGTACAAAA TGAAGAAAAC TATGCACAAG TTCTGGATAA GTTTGGAAGT 
AATTTTTTAA GCAGAGACAA CCCTGACCTT GGCACCGCTT TTGTCAAGTT TTCTACACTC 
ACAAAGGAAC TGTCCACGCT GCTGAAAAAT CTGCTCCAGG GTCTGAGCCA CAATGTGATC 
TTCACCTTGG ATTCCTTGTT AAAAGGAGAC TTGAAGGGGG TCAAAGGAGA TCTCAAGAAG 
CCATTTGACA AAGCCTGGAA AGATTATGAG ACGAAGTTTA CAAAAATTGA GAAGGAGAAA 
AGAGAGCACG CAAAACAGCA CGGGATGATC CGAACAGAGA TAACAGGCGC TGAGATTGCA 
GAAGAAATGG AAAAGGAACG GCGGCTTTTT CAGCTCCAGA TGTGTGAATA TCTCATTAAA 
GTAAATGAAA TCAAGACCAA AAAGGGTGTG GACCTGCTGC AGAACCTTAT AAAGTATTAC 
CATGCACAGT GCAATTTCTT TCAGGATGGC TTGAAAACGG CTGATAAACT GAAACAGTAC 
ATAGAAAAGC TGGCTGCTGA CTTATATAAT ATAAAGCAAA CCCAAGATGA AGAAAAAAAA 
CAGCTAACTG CACTCCGAGA TCTAATAAAA TCCTCTCTGC AACTTGATCC GAAAGAATCT 
AGAAGAGACT CTCAGAGCCG GCAAGGTGGG TACAGCATGC ATCAGCTCCA GGGCAACAAG 
GAGTACGGCA GTGAAAAGAA GGGCTTCCTG CTGAAGAAGA GTGATGGGAT CCGGAAAGTG 
TGGCAGAGAC GGAAGTGTGC GGTCAAGAAC GGGATCCTGA CCATCTCCCA CGCAACTTCC 
AACAGGCAGC CTGCTAAGTT AAACCTCCTC ACCTGCCAAG TGAAACCGAA TGCTGAGGAC 
AAGAAGTCTT TTGACCTGAT ATCACATAAT CGAACATATC ACTTTCAAGC AGAAGATGAG 
CAGGATTATA TAGCGTGGAT ATCAGTACTG ACGAATAGCA AAGAAGAGGC CCTAACCATG 
GCCTTCCGTG GTGAGCAGAG CACAGGGGAG AATAGCCTGG AGGACCTTAC CAAAGCCATC 
ATCGAGGATG TACAGCGGCT CCCTGGGAAT GACATCTGCT GTGACTGCGG CTCATCAGAA 
CCCACGTGGC TTTCAACCAA CTTGGGTATT TTGACCTGTA TAGAATGTTC CGGAATCCAT 
AGGGAAATGG GGGTTCATAT TTCTCGCATT CAGTCTTTGG AACTAGACAA ATTAGGAACT 
TCCGAACTCT TGCTGGCCAA GAATGTAGGA AACAATAGTT TTAATGATAT TATGGAAGCA 
AATTTACCCA GCCCTTCACC AAAGCCCACC CCTTCAAGTG ATATGACCGT GCGTAAAGAG 
TACATCACTG CAAAGTATGT CGACCACCGC TTCTCCAGGA AGACCTGTGC CTCTTCCTCA 
GCAAAGCTGA ACGAATTGCT TGAGGCCATC AAGTCCAGGG ACTTACTTGC ATTAATCCAA 
GTCTATGCAG AGGGGGTGGA GTTAATGGAG CCACTGCTGG AACCTGGGCA GGAGCTCGGG 
GAGACAGCCC TTCATCTTGC CGTCAGGACC GCAGACCAGA CATCTCTCCA TCTGGTTGAT 
TTCCTTGTAC AAAACTGTGG GAACCTGGAT AAGCAAACGT CTGTGGGGAA TACAGTTCTG 
CACTATTGCA GCATGTACGG CAAGCCGGAG TGTCTGAAGC TGCTGCTTAG GAGCAAGCCC 
ACCGTGGACA TCGTTAACCA GAATGGAGAA ACTGCCTTGG ATATAGCAAA GAGACTCAAA 
GCTACCCAGT GTGAAGATCT GCTTTCCCAG GCTAAATCTG GAAAGTTCAA CCCTCATGTC 
CACGTAGAGT ATGAGTGGAA TCTTCGACAG GACGAGATGG ATGAGAGCGA TGACGATCTG 
GATGACAAGC CAAGCCCGAT CAAAAAGGAA CGCTCGCCCA GACCACAGAG CTTCTGCCAC 
TCCTCCAGCA TCTCGCCACA GGACAAGCTG GCACTGCCAG GGTTCAGCAC TCCACGGGAC 
AAGCAGCGGC TCTCCTACGG AGCCTTCACC AACCAGATCT TCGCCTCTAC GAGCACAGAT 
TTGCCCACAT CACCCACCAG TGAGGCTCCC CCTTTGCCAC CTCGGAACGC CGGGAAAGGT 
AATGATGTGG GCCCATTATC TTCAAGTAAG ACGGCCAACA AGTTTGAGGG GCTGTCTCAG 
CAAGCAAGCA CCAGTTCTGC TAAGACTGCC CTTGGCCCGA GAGTGCTTCC TAAACTACCT 
CAGAAAGTGG CACTAAGGAA GACGGAGACC AGCCATCATC TCTCCCTCGA CAGAACCAAC 
ATCCCACCTG AGACTTTTCA GAAATCATCA CAGTTGACAG AGTTACCCCA AAAGCCACCA 
CTTGGAGAGC TGCCCCCGAA GCCTGTGGAA CTGGCCCCCA AGCCCCAAGT TGGAGAGCTG 
CCACCTAAGC CTGGAGAGCT ACCCCCTAAG CCCCAATTAG GTGACCTGCC CCCCAAGCCA 
CAGCTCTCAG ACTTACCTCC CAAGCCACAG ATGAAGGACC TGCCCCCTAA GCCGCAGCTG 
GGGGATCTGC TGGCAAAGTC CCAGGCTGGC GATGTCTCAG CCAAGGTGCA GCCACCCTCA 
GAGGTCACAC AGAGGTCACA CACCGGGGAT CTGTCTCCAA ATGTACAGTC CAGAGATGCC 
ATCCAGAAGC AAGCATCTGA AGACTCCAAC GACCTCACAC CCACGCTGCC AGAGACACCC 
GTACCACTGC CGAGAAAAAT CAATACGGGG AAAAATAAAG TGAGGCGGGT GAAGACCATT 
TATGACTGCC AGGCAGATAA TGACGACGAA CTCACATTTA TTGAGGGGGA AGTGATCATT 
GTCACCGGGG AAGAGGACCA GGAGTGGTGG ATCGGGCATA TCGAAGGACA GCCTGAAAGG 
AAGGGTGTCT TTCCAGTGTC CTTTGTCCAC ATCCTGTCTG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Erythroblast differentiation at spleen in Q137E mutant ribosomal protein S19 gene knock-in C57BL/6J mice.
Immunobiology223(1)118-124(2018 Jan)
Yamanegi K,Yamada N,Nakasho K,Nishiura H

The Arf GTPase-activating Protein, ASAP1, Binds Nonmuscle Myosin 2A to Control Remodeling of the Actomyosin Network.
The Journal of biological chemistry291(14)7517-26(2016 Apr)
Chen PW,Jian X,Heissler SM,Le K,Luo R,Jenkins LM,Nagy A,Moss J,Sellers JR,Randazzo PA

A pooled shRNA screen for regulators of primary mammary stem and progenitor cells identifies roles for Asap1 and Prox1.
BMC cancer15221(2015 Apr)
Sheridan JM,Ritchie ME,Best SA,Jiang K,Beck TJ,Vaillant F,Liu K,Dickins RA,Smyth GK,Lindeman GJ,Visvader JE

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Asap1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

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