ASAP1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	ASAP1
Entrez Gene ID	327705
Full Name	ArfGAP with SH3 domain, ankyrin repeat and PH domain 1
Synonyms	DDEF1,DEF-1
General protein information	Preferred Names ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 Names arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 130 kDa phosphatidylinositol 4,5-biphosphate-dependent ARF1 GTPase-activating protein 130 kDa phosphatidylinositol 4,5-bisphosphate-dependent ARF1 GTPase-activating protein ADP-ribosylation factor-directed GTPase-activating protein 1 ARF GTPase-activating protein 1 PIP2-dependent ARF1 GAP development and differentiation enhancing factor 1 differentiation-enhancing factor 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015474408.1	XP_015329894.1	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X1
XM_015474410.1	XP_015329896.1	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X3
XM_025001431.1	XP_024857199.1	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X2
XM_015474409.1	XP_015329895.1	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X2
NM_175821.2	NP_787015.1	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1
NM_175821.3	NP_787015.2	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1
XM_015474408.2	XP_015329894.1	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X1
XM_025001432.1	XP_024857200.1	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X3

Pathways from BioSystems

Homologies

	ASAP1	XP_001084688.1
Homo sapiens (human)	ASAP1	NP_060952.2
Gallus gallus (chicken)	ASAP1	XP_425945.4
Drosophila melanogaster (fruit fly)	Asap1	NP_001163085.1
Xenopus tropicalis (tropical clawed frog)	asap1	NP_001090735.1
Pan troglodytes (chimpanzee)	ASAP1	XP_003311959.1
Mus musculus (house mouse)	Asap1	NP_034156.2
Danio rerio (zebrafish)	asap1b	XP_005162660.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007726	XP_001687844.1
Canis lupus familiaris (dog)	ASAP1	XP_005627995.1
Bos taurus (cattle)	ASAP1	NP_787015.1
Rattus norvegicus (Norway rat)	Asap1	NP_001037710.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

DEF-1, a novel Src SH3 binding protein that promotes adipogenesis in fibroblastic cell lines.
King FJ, Hu E, Harris DF, Sarraf P, Spiegelman BM, Roberts TM
Molecular and cellular biology19(3)2330-7(1999 Mar)

ASAP1, a phospholipid-dependent arf GTPase-activating protein that associates with and is phosphorylated by Src.
Brown MT, Andrade J, Radhakrishna H, Donaldson JG, Cooper JA, Randazzo PA
Molecular and cellular biology18(12)7038-51(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ASAP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ASAP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa238848	NM_175821.3 Latest version!	Bos taurus ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (ASAP1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa222203	XM_015474408.2 Latest version!	Bos taurus ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (ASAP1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa222204	XM_025001431.1 Latest version!	Bos taurus ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (ASAP1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa222205	XM_025001432.1 Latest version!	Bos taurus ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (ASAP1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa41716	NM_175821.2 Latest version!	Bos taurus ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (ASAP1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa92491	XM_015474408.1 Latest version!	Bos taurus ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (ASAP1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa92492	XM_015474409.1 Latest version!	Bos taurus ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (ASAP1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa92493	XM_015474410.1 Latest version!	Bos taurus ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (ASAP1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ASAP1 ( NM_175821.3 ) cDNA ORF clone, Bos taurus(cattle) -> NP_787015.2 Bos taurus ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (ASAP1), mRNA.

Price & Availability↑

CloneID	OBa238848
Clone ID Related Accession (Same CDS sequence)	NM_175821.3
Accession Version	NM_175821.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3390bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-13
Organism	Bos taurus(cattle)
Product	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DAAA02037608.1, DAAA02037617.1 and EE241806.1. On Jan 19, 2018 this sequence version replaced NM_175821.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AF112886.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145437, SAMN03145449 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGATCTT CAGCCTCCAG GCTCTCCAGT TTTTCATCAA GAGATTCGCT ATGGAATCGG 
ATGCCGGACC AGATCTCCGT CTCCGAGTTC ATCGCCGAGA CCACCGAGGA CTACAACTCG 
CCCACCACGT CCAGCTTCAC TACGCGGCTG CACAACTGCA GGAACACCGT CACGCTGCTG 
GAGGAGGCTC TAGACCAAGA TAGAACAGCC TTACAGAAAG TTAAGAAGTC TGTAAAAGCA 
ATATACAATT CCGGTCAAGA CCATGTACAA AATGAAGAAA ACTATGCACA AGTTCTTGAT 
AAGTTTGGGA GTAATTTTTT AAGTCGAGAC AACCCAGATC TTGGCACCGC TTTTGTCAAG 
TTTTCTACGC TTACAAAGGA ACTGTCCACA CTGCTGAAAA ATCTGCTCCA GGGCCTGAGC 
CACAATGTGA TCTTCACCTT GGATTCCTTG TTGAAAGGAG ACCTGAAGGG AGTCAAAGGC 
GATCTCAAGA AACCATTTGA CAAAGCTTGG AAAGATTATG AGACGAAGTT TACCAAAATT 
GAGAAGGAGA AGAGGGAGCA CGCCAAGCAG CACGGGATGA TCCGCACGGA GATCACCGGC 
GCCGAGATCG CGGAGGAAAT GGAGAAGGAG CGGCGCCTCT TCCAGCTCCA GATGTGCGAG 
TATCTCATTA AAGTTAATGA AATCAAGACC AAAAAGGGTG TGGATCTGCT GCAGAACCTG 
ATAAAGTATT ATCACGCACA GTGCAATTTC TTTCAAGATG GTTTGAAAAC AGCTGATAAA 
TTGAAACAGT ACATTGAAAA GCTGGCTGCT GATTTGTATA ATATCAAACA GACCCAGGAC 
GAAGAAAAGA AACAGCTGAC CGCACTCCGA GACCTAATAA AGTCCTCGCT CCAACTCGAT 
CAGAAAGAAT CTAGGAGAGA TTCCCAGAGC CGGCAGGGAG GCTACAGCAT GCACCAGCTG 
CAGGGCAACA AGGAATACGG CAGCGAGAAG AAGGGCTACC TGCTGAAGAA GAGTGACGGG 
ATCCGGAAAG TGTGGCAGAG AAGGAAGTGC TCCGTCAAGA ACGGGATCCT GACCATCTCC 
CACGCCACGT CCAACAGACA GCCAGCCAAG CTGAACCTTC TCACTTGCCA GGTGAAGCCG 
AATGCCGAGG ACAAGAAGTC TTTCGACCTG ATATCACATA ACAGGACGTA TCACTTTCAG 
GCCGAAGATG AGCAGGATTA TGTAGCGTGG ATCTCGGTGC TGACGAACAG CAAAGAGGAG 
GCCCTCACCA TGGCCTTCCG GGGGGAACAG AGTGCTGGGG AGAGCAGCCT GGAGGAGCTG 
ACGAAGGCCA TCATCGAGGA CGTGCAGCGG CTCCCGGGCA ACGACGTCTG CTGCGACTGC 
GGCTCGGCAG AACCCACCTG GCTGTCCACC AACTTGGGTA TCTTGACCTG TATAGAATGT 
TCCGGCATCC ATAGAGAAAT GGGGGTTCAT ATTTCTCGCA TCCAGTCTTT GGAACTAGAC 
AAATTAGGAA CTTCTGAACT CTTGCTGGCC AAGAATGTAG GAAACAATAG TTTTAATGAT 
ATTATGGAAG CAAATTTACC CAGTCCCTCA CCAAAACCCA CCCCTTCAAG TGATATGACC 
GTACGGAAGG AATATATCAC TGCAAAGTAT GTAGATCATA GGTTTTCACG GAAGACCTGT 
TCATCCTCAT CAGCTAAACT GAACGAATTG CTTGAGGCCA TCAAATCCAG GGATTTACTT 
GCACTAATTC AAGTCTATGC AGAGGGGGTG GAGCTAATGG AACCGCTGCT GGAACCCGGA 
CAGGAGCTTG GGGAGACAGC CCTTCATCTT GCAGTCCGAA CCGCAGACCA GACATCTCTC 
CATTTGGTGG ACTTCCTTGT ACAAAACTGT GGGAACCTAG ATAAGCAGAC GGCCCTGGGG 
AACACGGCCC TGCACTACTG TAGTATGTAC AGTAAACCAG AGTGTTTGAA GCTGCTGCTC 
AGGAGCAAGC CCACTGTGGA CGTCGTTAAT CAGGCTGGAG AGACCGCCCT GGACATAGCA 
AAGAGACTGA AAGCCACTCA GTGTGAAGAC CTGCTTTCCC AAGCTAAATC TGGAAAGTTC 
AATCCACACG TCCACGTGGA ATATGAGTGG AATCTTCGAC AGGAGGAGAT GGATGAGAGC 
GATGACGACC TGGATGACAA ACCGAGCCCC ATCAAGAAGG AGCGCTCCCC CCGACCGCAG 
AGCTTCTGCC ACTCCTCCAG CATCTCCCCC CAGGACAAGC TCTCACTGCC GGGCTTCAGC 
ACGCCAAGGG ACAAGCAACG ACTCTCCTAC GGCGCCTTCA CCAACCAGAT CTTCGTCTCC 
ACAAGCACAG ACTCACCCAC GTCACCGATC GCAGAGGCGC CCCCGCTGCC TCCCAGAAAC 
GCCACGAAAG GTCCACCTGG CCCACCTTCA ACACTCCCTC TAAGCACCCA GACCTCTAGT 
GGCAGCTCCA CCCTGTCCAA GAAGCGGCCT CCTCCCCCAC CACCCGGACA CAAGAGAACC 
CTGTCTGACC CTCCCAGCCC ACTACCTCAC GGGCCCCCAA ACAAAGGCGC AGTTCCTTGG 
GGTAACGACG TGGGTCCCTC ATCGTCCAGT AAGACCACGA ACAAGTTCGA GGGCCTGTCC 
CAGCAGTCGA GCACCGGTTC TGCAAAGACT GCACTTGGCC CAAGAGTTCT TCCTAAACTA 
CCTCAGAAAG TGGCACTAAG GAAAACAGAG ACCAGCCATC ATCTCTCCCT CGACAAAGCC 
AACGTCCCAC CTGAGATCTT CCAGAAGTCG TCCCAGTTGA CAGAGTTACC GCAGAAGCCG 
CCACCCGGGG ACCTGCCCCC GAAGCCCACG GAACTGGCTC CCAAACCCCC CATTGGAGAC 
TTACCACCTA AGCCAGGCGA GCTGCCCCCG AAGCCACAGC TGGGCGACCT GCCCCCCAAG 
CCCCAGCTCG CAGACTTGCC CCCCAAGCCC CAGGTGAAAG ACCTGCCTCC CAAGCCACAA 
CTGGGGGAGC TGCTGGCAAA ACCCCAGACG GGAGACGCCT CGCCCAAGGC CCAGCCACCC 
CTGGAGCTCA CCCCCAAGTC ACACCCGGCG GACCTGTCCC CGAACGTCCC CAAGCAGGCG 
TCTGAGGACA CCAACGACCT CACGCCCACC CTGCCAGAGA CACCTGTGCC GCTGCCCAGG 
AAGATCAACA CGGGGAAGAG CAAGGTGAGG CGAGTGAAGA CCATCTACGA CTGCCAGGCG 
GACAACGATG ACGAGCTGAC TTTCATGGAG GGCGAGGTGA TCGTGGTCAC CGGGGAGGAG 
GACCAGGAGT GGTGGATTGG GCACATCGAG GGGCAGCCCG AGAGGAAGGG CGTCTTCCCA 
GTGTCCTTTG TCCACATCCT GTCGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_787015.2
CDS	209..3598
Translation	MRSSASRLSSFSSRDSLWNRMPDQISVSEFIAETTEDYNSPTTSSFTTRLHNCRNTVTLLEEALDQDRTALQKVKKSVKAIYNSGQDHVQNEENYAQVLDKFGSNFLSRDNPDLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGVKGDLKKPFDKAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDQKESRRDSQSRQGGYSMHQLQGNKEYGSEKKGYLLKKSDGIRKVWQRRKCSVKNGILTISHATSNRQPAKLNLLTCQVKPNAEDKKSFDLISHNRTYHFQAEDEQDYVAWISVLTNSKEEALTMAFRGEQSAGESSLEELTKAIIEDVQRLPGNDVCCDCGSAEPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDKLGTSELLLAKNVGNNSFNDIMEANLPSPSPKPTPSSDMTVRKEYITAKYVDHRFSRKTCSSSSAKLNELLEAIKSRDLLALIQVYAEGVELMEPLLEPGQELGETALHLAVRTADQTSLHLVDFLVQNCGNLDKQTALGNTALHYCSMYSKPECLKLLLRSKPTVDVVNQAGETALDIAKRLKATQCEDLLSQAKSGKFNPHVHVEYEWNLRQEEMDESDDDLDDKPSPIKKERSPRPQSFCHSSSISPQDKLSLPGFSTPRDKQRLSYGAFTNQIFVSTSTDSPTSPIAEAPPLPPRNATKGPPGPPSTLPLSTQTSSGSSTLSKKRPPPPPPGHKRTLSDPPSPLPHGPPNKGAVPWGNDVGPSSSSKTTNKFEGLSQQSSTGSAKTALGPRVLPKLPQKVALRKTETSHHLSLDKANVPPEIFQKSSQLTELPQKPPPGDLPPKPTELAPKPPIGDLPPKPGELPPKPQLGDLPPKPQLADLPPKPQVKDLPPKPQLGELLAKPQTGDASPKAQPPLELTPKSHPADLSPNVPKQASEDTNDLTPTLPETPVPLPRKINTGKSKVRRVKTIYDCQADNDDELTFMEGEVIVVTGEEDQEWWIGHIEGQPERKGVFPVSFVHILSD

Target ORF information:

RefSeq Version	NM_175821.3
Organism	Bos taurus(cattle)
Definition	Bos taurus ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (ASAP1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175821.3

ORF Insert Sequence:

ATGAGATCTT CAGCCTCCAG GCTCTCCAGT TTTTCATCAA GAGATTCGCT ATGGAATCGG 
ATGCCGGACC AGATCTCCGT CTCCGAGTTC ATCGCCGAGA CCACCGAGGA CTACAACTCG 
CCCACCACGT CCAGCTTCAC TACGCGGCTG CACAACTGCA GGAACACCGT CACGCTGCTG 
GAGGAGGCTC TAGACCAAGA TAGAACAGCC TTACAGAAAG TTAAGAAGTC TGTAAAAGCA 
ATATACAATT CCGGTCAAGA CCATGTACAA AATGAAGAAA ACTATGCACA AGTTCTTGAT 
AAGTTTGGGA GTAATTTTTT AAGTCGAGAC AACCCAGATC TTGGCACCGC TTTTGTCAAG 
TTTTCTACGC TTACAAAGGA ACTGTCCACA CTGCTGAAAA ATCTGCTCCA GGGCCTGAGC 
CACAATGTGA TCTTCACCTT GGATTCCTTG TTGAAAGGAG ACCTGAAGGG AGTCAAAGGC 
GATCTCAAGA AACCATTTGA CAAAGCTTGG AAAGATTATG AGACGAAGTT TACCAAAATT 
GAGAAGGAGA AGAGGGAGCA CGCCAAGCAG CACGGGATGA TCCGCACGGA GATCACCGGC 
GCCGAGATCG CGGAGGAAAT GGAGAAGGAG CGGCGCCTCT TCCAGCTCCA GATGTGCGAG 
TATCTCATTA AAGTTAATGA AATCAAGACC AAAAAGGGTG TGGATCTGCT GCAGAACCTG 
ATAAAGTATT ATCACGCACA GTGCAATTTC TTTCAAGATG GTTTGAAAAC AGCTGATAAA 
TTGAAACAGT ACATTGAAAA GCTGGCTGCT GATTTGTATA ATATCAAACA GACCCAGGAC 
GAAGAAAAGA AACAGCTGAC CGCACTCCGA GACCTAATAA AGTCCTCGCT CCAACTCGAT 
CAGAAAGAAT CTAGGAGAGA TTCCCAGAGC CGGCAGGGAG GCTACAGCAT GCACCAGCTG 
CAGGGCAACA AGGAATACGG CAGCGAGAAG AAGGGCTACC TGCTGAAGAA GAGTGACGGG 
ATCCGGAAAG TGTGGCAGAG AAGGAAGTGC TCCGTCAAGA ACGGGATCCT GACCATCTCC 
CACGCCACGT CCAACAGACA GCCAGCCAAG CTGAACCTTC TCACTTGCCA GGTGAAGCCG 
AATGCCGAGG ACAAGAAGTC TTTCGACCTG ATATCACATA ACAGGACGTA TCACTTTCAG 
GCCGAAGATG AGCAGGATTA TGTAGCGTGG ATCTCGGTGC TGACGAACAG CAAAGAGGAG 
GCCCTCACCA TGGCCTTCCG GGGGGAACAG AGTGCTGGGG AGAGCAGCCT GGAGGAGCTG 
ACGAAGGCCA TCATCGAGGA CGTGCAGCGG CTCCCGGGCA ACGACGTCTG CTGCGACTGC 
GGCTCGGCAG AACCCACCTG GCTGTCCACC AACTTGGGTA TCTTGACCTG TATAGAATGT 
TCCGGCATCC ATAGAGAAAT GGGGGTTCAT ATTTCTCGCA TCCAGTCTTT GGAACTAGAC 
AAATTAGGAA CTTCTGAACT CTTGCTGGCC AAGAATGTAG GAAACAATAG TTTTAATGAT 
ATTATGGAAG CAAATTTACC CAGTCCCTCA CCAAAACCCA CCCCTTCAAG TGATATGACC 
GTACGGAAGG AATATATCAC TGCAAAGTAT GTAGATCATA GGTTTTCACG GAAGACCTGT 
TCATCCTCAT CAGCTAAACT GAACGAATTG CTTGAGGCCA TCAAATCCAG GGATTTACTT 
GCACTAATTC AAGTCTATGC AGAGGGGGTG GAGCTAATGG AACCGCTGCT GGAACCCGGA 
CAGGAGCTTG GGGAGACAGC CCTTCATCTT GCAGTCCGAA CCGCAGACCA GACATCTCTC 
CATTTGGTGG ACTTCCTTGT ACAAAACTGT GGGAACCTAG ATAAGCAGAC GGCCCTGGGG 
AACACGGCCC TGCACTACTG TAGTATGTAC AGTAAACCAG AGTGTTTGAA GCTGCTGCTC 
AGGAGCAAGC CCACTGTGGA CGTCGTTAAT CAGGCTGGAG AGACCGCCCT GGACATAGCA 
AAGAGACTGA AAGCCACTCA GTGTGAAGAC CTGCTTTCCC AAGCTAAATC TGGAAAGTTC 
AATCCACACG TCCACGTGGA ATATGAGTGG AATCTTCGAC AGGAGGAGAT GGATGAGAGC 
GATGACGACC TGGATGACAA ACCGAGCCCC ATCAAGAAGG AGCGCTCCCC CCGACCGCAG 
AGCTTCTGCC ACTCCTCCAG CATCTCCCCC CAGGACAAGC TCTCACTGCC GGGCTTCAGC 
ACGCCAAGGG ACAAGCAACG ACTCTCCTAC GGCGCCTTCA CCAACCAGAT CTTCGTCTCC 
ACAAGCACAG ACTCACCCAC GTCACCGATC GCAGAGGCGC CCCCGCTGCC TCCCAGAAAC 
GCCACGAAAG GTCCACCTGG CCCACCTTCA ACACTCCCTC TAAGCACCCA GACCTCTAGT 
GGCAGCTCCA CCCTGTCCAA GAAGCGGCCT CCTCCCCCAC CACCCGGACA CAAGAGAACC 
CTGTCTGACC CTCCCAGCCC ACTACCTCAC GGGCCCCCAA ACAAAGGCGC AGTTCCTTGG 
GGTAACGACG TGGGTCCCTC ATCGTCCAGT AAGACCACGA ACAAGTTCGA GGGCCTGTCC 
CAGCAGTCGA GCACCGGTTC TGCAAAGACT GCACTTGGCC CAAGAGTTCT TCCTAAACTA 
CCTCAGAAAG TGGCACTAAG GAAAACAGAG ACCAGCCATC ATCTCTCCCT CGACAAAGCC 
AACGTCCCAC CTGAGATCTT CCAGAAGTCG TCCCAGTTGA CAGAGTTACC GCAGAAGCCG 
CCACCCGGGG ACCTGCCCCC GAAGCCCACG GAACTGGCTC CCAAACCCCC CATTGGAGAC 
TTACCACCTA AGCCAGGCGA GCTGCCCCCG AAGCCACAGC TGGGCGACCT GCCCCCCAAG 
CCCCAGCTCG CAGACTTGCC CCCCAAGCCC CAGGTGAAAG ACCTGCCTCC CAAGCCACAA 
CTGGGGGAGC TGCTGGCAAA ACCCCAGACG GGAGACGCCT CGCCCAAGGC CCAGCCACCC 
CTGGAGCTCA CCCCCAAGTC ACACCCGGCG GACCTGTCCC CGAACGTCCC CAAGCAGGCG 
TCTGAGGACA CCAACGACCT CACGCCCACC CTGCCAGAGA CACCTGTGCC GCTGCCCAGG 
AAGATCAACA CGGGGAAGAG CAAGGTGAGG CGAGTGAAGA CCATCTACGA CTGCCAGGCG 
GACAACGATG ACGAGCTGAC TTTCATGGAG GGCGAGGTGA TCGTGGTCAC CGGGGAGGAG 
GACCAGGAGT GGTGGATTGG GCACATCGAG GGGCAGCCCG AGAGGAAGGG CGTCTTCCCA 
GTGTCCTTTG TCCACATCCT GTCGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASAP1 ( XM_015474408.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329894.1 Bos taurus ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (ASAP1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa222203
Clone ID Related Accession (Same CDS sequence)	XM_015474408.2
Accession Version	XM_015474408.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3381bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015474408.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATCTT CAGCCTCCAG GCTCTCCAGT TTTTCATCAA GAGATTCGCT ATGGAATCGG 
ATGCCGGACC AGATCTCCGT CTCCGAGTTC ATCGCCGAGA CCACCGAGGA CTACAACTCG 
CCCACCACGT CCAGCTTCAC TACGCGGCTG CACAACTGCA GGAACACCGT CACGCTGCTG 
GAGGAGGCTC TAGACCAAGA TAGAACAGCC TTACAGAAAG TTAAGAAGTC TGTAAAAGCA 
ATATATAATT CCGGTCAAGA CCATGTACAA AATGAAGAAA ACTATGCACA AGTTCTTGAT 
AAGTTTGGGA GTAATTTTTT AAGTCGAGAC AACCCAGATC TTGGCACCGC TTTTGTCAAG 
TTTTCTACGC TTACAAAGGA ACTGTCCACA CTGCTGAAAA ATCTGCTCCA GGGCCTGAGC 
CACAATGTGA TCTTCACCTT GGATTCCTTG TTGAAAGGAG ACCTGAAGGG AGTCAAAGGC 
GATCTCAAGA AACCATTTGA CAAAGCTTGG AAAGATTATG AGACGAAGTT TACCAAAATT 
GAGAAGGAGA AGAGGGAGCA CGCCAAGCAG CACGGGATGA TCCGCACGGA GATCACCGGC 
GCCGAGATCG CGGAGGAAAT GGAGAAGGAG CGGCGCCTCT TCCAGCTCCA GATGTGCGAG 
TATCTCATTA AAGTTAATGA AATCAAGACC AAAAAGGGTG TGGATCTGCT GCAGAACCTG 
ATAAAGTATT ATCACGCACA GTGCAATTTC TTTCAAGATG GTTTGAAAAC AGCTGATAAA 
TTGAAACAGT ACATTGAAAA GCTGGCTGCT GATTTGTATA ATATCAAACA GACCCAGGAC 
GAAGAAAAGA AACAGCTGAC CGCACTCCGA GACCTAATAA AGTCCTCGCT CCAACTCGAT 
CAGAAAGAAG ATTCCCAGAG CCGGCAGGGA GGCTACAGCA TGCACCAGCT GCAGGGCAAC 
AAGGAATACG GCAGCGAGAA GAAGGGCTAC CTGCTGAAGA AGAGTGACGG GATCCGGAAA 
GTGTGGCAGA GAAGGAAGTG CTCCGTCAAG AACGGGATCC TGACCATCTC CCACGCCACG 
TCCAACAGAC AGCCAGCCAA GCTGAACCTT CTCACTTGCC AGGTGAAGCC GAATGCCGAG 
GACAAGAAGT CTTTCGACCT GATATCACAT AACAGGACGT ATCACTTTCA GGCCGAAGAT 
GAGCAGGATT ATGTAGCGTG GATCTCGGTG CTGACGAACA GCAAAGAGGA GGCCCTCACC 
ATGGCCTTCC GGGGGGAACA GAGTGCTGGG GAGAGCAGCC TGGAGGAGCT GACGAAGGCC 
ATCATCGAGG ACGTGCAGCG GCTCCCGGGC AACGACGTCT GCTGCGACTG CGGCTCGGCA 
GAACCCACCT GGCTGTCCAC CAACTTGGGT ATCTTGACCT GTATAGAATG TTCCGGCATC 
CATAGAGAAA TGGGGGTTCA TATTTCTCGC ATCCAGTCTT TGGAACTAGA CAAATTAGGA 
ACTTCTGAAC TCTTGCTGGC CAAGAATGTA GGAAACAATA GTTTTAATGA TATTATGGAA 
GCAAATTTAC CCAGTCCCTC ACCAAAACCC ACCCCTTCAA GTGATATGAC CGTACGGAAG 
GAATATATCA CTGCAAAGTA TGTAGATCAT AGGTTTTCAC GGAAGACCTG TTCATCCTCA 
TCAGCTAAAC TGAACGAATT GCTTGAGGCC ATCAAATCCA GGGATTTACT TGCACTAATT 
CAAGTCTATG CAGAGGGGGT GGAGCTAATG GAACCGCTGC TGGAACCCGG ACAGGAGCTT 
GGGGAGACAG CCCTTCATCT TGCAGTCCGA ACCGCAGACC AGACATCTCT CCATTTGGTG 
GACTTCCTTG TACAAAACTG TGGGAACCTA GATAAGCAGA CGGCCCTGGG GAACACGGCC 
CTGCACTACT GTAGTATGTA CAGTAAACCA GAGTGTTTGA AGCTGCTGCT CAGGAGCAAG 
CCCACTGTGG ACGTCGTTAA TCAGGCTGGA GAGACCGCCC TGGACATAGC AAAGAGACTG 
AAAGCCACTC AGTGTGAAGA CCTGCTTTCC CAAGCTAAAT CTGGAAAGTT CAATCCACAC 
GTCCACGTGG AATATGAGTG GAATCTTCGA CAGGAGGAGA TGGATGAGAG CGATGACGAC 
CTGGATGACA AACCGAGCCC CATCAAGAAG GAGCGCTCCC CCCGACCGCA GAGCTTCTGC 
CACTCCTCCA GCATCTCCCC CCAGGACAAG CTCTCACTGC CGGGCTTCAG CACGCCAAGG 
GACAAGCAAC GACTCTCCTA CGGCGCCTTC ACCAACCAGA TCTTCGTCTC CACAAGCACA 
GACTCACCCA CGTCACCGAT CGCAGAGGCG CCCCCGCTGC CTCCCAGAAA CGCCACGAAA 
GGTCCACCTG GCCCACCTTC AACACTCCCT CTAAGCACCC AGACCTCTAG TGGCAGCTCC 
ACCCTGTCCA AGAAGCGGCC TCCTCCCCCA CCACCCGGAC ACAAGAGAAC CCTGTCTGAC 
CCTCCCAGCC CACTACCTCA CGGGCCCCCA AACAAAGGCG CAGTTCCTTG GGGTAACGAC 
GTGGGTCCCT CATCGTCCAG TAAGACCACG AACAAGTTCG AGGGCCTGTC CCAGCAGTCG 
AGCACCGGTT CTGCAAAGAC TGCACTTGGC CCAAGAGTTC TTCCTAAACT ACCTCAGAAA 
GTGGCACTAA GGAAAACAGA GACCAGCCAT CATCTCTCCC TCGACAAAGC CAACGTCCCA 
CCTGAGATCT TCCAGAAGTC GTCCCAGTTG ACAGAGTTAC CGCAGAAGCC GCCACCCGGG 
GACCTGCCCC CGAAGCCCAC GGAACTGGCT CCCAAACCCC CCATTGGAGA CTTACCACCT 
AAGCCAGGCG AGCTGCCCCC GAAGCCACAG CTGGGCGACC TGCCCCCCAA GCCCCAGCTC 
GCAGACTTGC CCCCCAAGCC CCAGGTGAAA GACCTGCCTC CCAAGCCACA ACTGGGGGAG 
CTGCTGGCAA AACCCCAGAC GGGAGACGCC TCGCCCAAGG CCCAGCCACC CCTGGAGCTC 
ACCCCCAAGT CACACCCGGC GGACCTGTCC CCGAACGTCC CCAAGCAGGC GTCTGAGGAC 
ACCAACGACC TCACGCCCAC CCTGCCAGAG ACACCTGTGC CGCTGCCCAG GAAGATCAAC 
ACGGGGAAGA GCAAGGTGAG GCGAGTGAAG ACCATCTACG ACTGCCAGGC GGACAACGAT 
GACGAGCTGA CTTTCATGGA GGGCGAGGTG ATCGTGGTCA CCGGGGAGGA GGACCAGGAG 
TGGTGGATTG GGCACATCGA GGGGCAGCCC GAGAGGAAGG GCGTCTTCCC AGTGTCCTTT 
GTCCACATCC TGTCGGACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329894.1
CDS	209..3589
Translation	MRSSASRLSSFSSRDSLWNRMPDQISVSEFIAETTEDYNSPTTSSFTTRLHNCRNTVTLLEEALDQDRTALQKVKKSVKAIYNSGQDHVQNEENYAQVLDKFGSNFLSRDNPDLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGVKGDLKKPFDKAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDQKEDSQSRQGGYSMHQLQGNKEYGSEKKGYLLKKSDGIRKVWQRRKCSVKNGILTISHATSNRQPAKLNLLTCQVKPNAEDKKSFDLISHNRTYHFQAEDEQDYVAWISVLTNSKEEALTMAFRGEQSAGESSLEELTKAIIEDVQRLPGNDVCCDCGSAEPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDKLGTSELLLAKNVGNNSFNDIMEANLPSPSPKPTPSSDMTVRKEYITAKYVDHRFSRKTCSSSSAKLNELLEAIKSRDLLALIQVYAEGVELMEPLLEPGQELGETALHLAVRTADQTSLHLVDFLVQNCGNLDKQTALGNTALHYCSMYSKPECLKLLLRSKPTVDVVNQAGETALDIAKRLKATQCEDLLSQAKSGKFNPHVHVEYEWNLRQEEMDESDDDLDDKPSPIKKERSPRPQSFCHSSSISPQDKLSLPGFSTPRDKQRLSYGAFTNQIFVSTSTDSPTSPIAEAPPLPPRNATKGPPGPPSTLPLSTQTSSGSSTLSKKRPPPPPPGHKRTLSDPPSPLPHGPPNKGAVPWGNDVGPSSSSKTTNKFEGLSQQSSTGSAKTALGPRVLPKLPQKVALRKTETSHHLSLDKANVPPEIFQKSSQLTELPQKPPPGDLPPKPTELAPKPPIGDLPPKPGELPPKPQLGDLPPKPQLADLPPKPQVKDLPPKPQLGELLAKPQTGDASPKAQPPLELTPKSHPADLSPNVPKQASEDTNDLTPTLPETPVPLPRKINTGKSKVRRVKTIYDCQADNDDELTFMEGEVIVVTGEEDQEWWIGHIEGQPERKGVFPVSFVHILSD

Target ORF information:

RefSeq Version	XM_015474408.2
Organism	Bos taurus(cattle)
Definition	Bos taurus ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (ASAP1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474408.2

ORF Insert Sequence:

ATGAGATCTT CAGCCTCCAG GCTCTCCAGT TTTTCATCAA GAGATTCGCT ATGGAATCGG 
ATGCCGGACC AGATCTCCGT CTCCGAGTTC ATCGCCGAGA CCACCGAGGA CTACAACTCG 
CCCACCACGT CCAGCTTCAC TACGCGGCTG CACAACTGCA GGAACACCGT CACGCTGCTG 
GAGGAGGCTC TAGACCAAGA TAGAACAGCC TTACAGAAAG TTAAGAAGTC TGTAAAAGCA 
ATATATAATT CCGGTCAAGA CCATGTACAA AATGAAGAAA ACTATGCACA AGTTCTTGAT 
AAGTTTGGGA GTAATTTTTT AAGTCGAGAC AACCCAGATC TTGGCACCGC TTTTGTCAAG 
TTTTCTACGC TTACAAAGGA ACTGTCCACA CTGCTGAAAA ATCTGCTCCA GGGCCTGAGC 
CACAATGTGA TCTTCACCTT GGATTCCTTG TTGAAAGGAG ACCTGAAGGG AGTCAAAGGC 
GATCTCAAGA AACCATTTGA CAAAGCTTGG AAAGATTATG AGACGAAGTT TACCAAAATT 
GAGAAGGAGA AGAGGGAGCA CGCCAAGCAG CACGGGATGA TCCGCACGGA GATCACCGGC 
GCCGAGATCG CGGAGGAAAT GGAGAAGGAG CGGCGCCTCT TCCAGCTCCA GATGTGCGAG 
TATCTCATTA AAGTTAATGA AATCAAGACC AAAAAGGGTG TGGATCTGCT GCAGAACCTG 
ATAAAGTATT ATCACGCACA GTGCAATTTC TTTCAAGATG GTTTGAAAAC AGCTGATAAA 
TTGAAACAGT ACATTGAAAA GCTGGCTGCT GATTTGTATA ATATCAAACA GACCCAGGAC 
GAAGAAAAGA AACAGCTGAC CGCACTCCGA GACCTAATAA AGTCCTCGCT CCAACTCGAT 
CAGAAAGAAG ATTCCCAGAG CCGGCAGGGA GGCTACAGCA TGCACCAGCT GCAGGGCAAC 
AAGGAATACG GCAGCGAGAA GAAGGGCTAC CTGCTGAAGA AGAGTGACGG GATCCGGAAA 
GTGTGGCAGA GAAGGAAGTG CTCCGTCAAG AACGGGATCC TGACCATCTC CCACGCCACG 
TCCAACAGAC AGCCAGCCAA GCTGAACCTT CTCACTTGCC AGGTGAAGCC GAATGCCGAG 
GACAAGAAGT CTTTCGACCT GATATCACAT AACAGGACGT ATCACTTTCA GGCCGAAGAT 
GAGCAGGATT ATGTAGCGTG GATCTCGGTG CTGACGAACA GCAAAGAGGA GGCCCTCACC 
ATGGCCTTCC GGGGGGAACA GAGTGCTGGG GAGAGCAGCC TGGAGGAGCT GACGAAGGCC 
ATCATCGAGG ACGTGCAGCG GCTCCCGGGC AACGACGTCT GCTGCGACTG CGGCTCGGCA 
GAACCCACCT GGCTGTCCAC CAACTTGGGT ATCTTGACCT GTATAGAATG TTCCGGCATC 
CATAGAGAAA TGGGGGTTCA TATTTCTCGC ATCCAGTCTT TGGAACTAGA CAAATTAGGA 
ACTTCTGAAC TCTTGCTGGC CAAGAATGTA GGAAACAATA GTTTTAATGA TATTATGGAA 
GCAAATTTAC CCAGTCCCTC ACCAAAACCC ACCCCTTCAA GTGATATGAC CGTACGGAAG 
GAATATATCA CTGCAAAGTA TGTAGATCAT AGGTTTTCAC GGAAGACCTG TTCATCCTCA 
TCAGCTAAAC TGAACGAATT GCTTGAGGCC ATCAAATCCA GGGATTTACT TGCACTAATT 
CAAGTCTATG CAGAGGGGGT GGAGCTAATG GAACCGCTGC TGGAACCCGG ACAGGAGCTT 
GGGGAGACAG CCCTTCATCT TGCAGTCCGA ACCGCAGACC AGACATCTCT CCATTTGGTG 
GACTTCCTTG TACAAAACTG TGGGAACCTA GATAAGCAGA CGGCCCTGGG GAACACGGCC 
CTGCACTACT GTAGTATGTA CAGTAAACCA GAGTGTTTGA AGCTGCTGCT CAGGAGCAAG 
CCCACTGTGG ACGTCGTTAA TCAGGCTGGA GAGACCGCCC TGGACATAGC AAAGAGACTG 
AAAGCCACTC AGTGTGAAGA CCTGCTTTCC CAAGCTAAAT CTGGAAAGTT CAATCCACAC 
GTCCACGTGG AATATGAGTG GAATCTTCGA CAGGAGGAGA TGGATGAGAG CGATGACGAC 
CTGGATGACA AACCGAGCCC CATCAAGAAG GAGCGCTCCC CCCGACCGCA GAGCTTCTGC 
CACTCCTCCA GCATCTCCCC CCAGGACAAG CTCTCACTGC CGGGCTTCAG CACGCCAAGG 
GACAAGCAAC GACTCTCCTA CGGCGCCTTC ACCAACCAGA TCTTCGTCTC CACAAGCACA 
GACTCACCCA CGTCACCGAT CGCAGAGGCG CCCCCGCTGC CTCCCAGAAA CGCCACGAAA 
GGTCCACCTG GCCCACCTTC AACACTCCCT CTAAGCACCC AGACCTCTAG TGGCAGCTCC 
ACCCTGTCCA AGAAGCGGCC TCCTCCCCCA CCACCCGGAC ACAAGAGAAC CCTGTCTGAC 
CCTCCCAGCC CACTACCTCA CGGGCCCCCA AACAAAGGCG CAGTTCCTTG GGGTAACGAC 
GTGGGTCCCT CATCGTCCAG TAAGACCACG AACAAGTTCG AGGGCCTGTC CCAGCAGTCG 
AGCACCGGTT CTGCAAAGAC TGCACTTGGC CCAAGAGTTC TTCCTAAACT ACCTCAGAAA 
GTGGCACTAA GGAAAACAGA GACCAGCCAT CATCTCTCCC TCGACAAAGC CAACGTCCCA 
CCTGAGATCT TCCAGAAGTC GTCCCAGTTG ACAGAGTTAC CGCAGAAGCC GCCACCCGGG 
GACCTGCCCC CGAAGCCCAC GGAACTGGCT CCCAAACCCC CCATTGGAGA CTTACCACCT 
AAGCCAGGCG AGCTGCCCCC GAAGCCACAG CTGGGCGACC TGCCCCCCAA GCCCCAGCTC 
GCAGACTTGC CCCCCAAGCC CCAGGTGAAA GACCTGCCTC CCAAGCCACA ACTGGGGGAG 
CTGCTGGCAA AACCCCAGAC GGGAGACGCC TCGCCCAAGG CCCAGCCACC CCTGGAGCTC 
ACCCCCAAGT CACACCCGGC GGACCTGTCC CCGAACGTCC CCAAGCAGGC GTCTGAGGAC 
ACCAACGACC TCACGCCCAC CCTGCCAGAG ACACCTGTGC CGCTGCCCAG GAAGATCAAC 
ACGGGGAAGA GCAAGGTGAG GCGAGTGAAG ACCATCTACG ACTGCCAGGC GGACAACGAT 
GACGAGCTGA CTTTCATGGA GGGCGAGGTG ATCGTGGTCA CCGGGGAGGA GGACCAGGAG 
TGGTGGATTG GGCACATCGA GGGGCAGCCC GAGAGGAAGG GCGTCTTCCC AGTGTCCTTT 
GTCCACATCC TGTCGGACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASAP1 ( XM_025001431.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024857199.1 Bos taurus ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (ASAP1), transcript variant X2, mRNA.

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CloneID	OBa222204
Clone ID Related Accession (Same CDS sequence)	XM_025001431.1
Accession Version	XM_025001431.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3321bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGACC AGATCTCCGT CTCCGAGTTC ATCGCCGAGA CCACCGAGGA CTACAACTCG 
CCCACCACGT CCAGCTTCAC TACGCGGCTG CACAACTGCA GGAACACCGT CACGCTGCTG 
GAGGAGGCTC TAGACCAAGA TAGAACAGCC TTACAGAAAG TTAAGAAGTC TGTAAAAGCA 
ATATATAATT CCGGTCAAGA CCATGTACAA AATGAAGAAA ACTATGCACA AGTTCTTGAT 
AAGTTTGGGA GTAATTTTTT AAGTCGAGAC AACCCAGATC TTGGCACCGC TTTTGTCAAG 
TTTTCTACGC TTACAAAGGA ACTGTCCACA CTGCTGAAAA ATCTGCTCCA GGGCCTGAGC 
CACAATGTGA TCTTCACCTT GGATTCCTTG TTGAAAGGAG ACCTGAAGGG AGTCAAAGGC 
GATCTCAAGA AACCATTTGA CAAAGCTTGG AAAGATTATG AGACGAAGTT TACCAAAATT 
GAGAAGGAGA AGAGGGAGCA CGCCAAGCAG CACGGGATGA TCCGCACGGA GATCACCGGC 
GCCGAGATCG CGGAGGAAAT GGAGAAGGAG CGGCGCCTCT TCCAGCTCCA GATGTGCGAG 
TATCTCATTA AAGTTAATGA AATCAAGACC AAAAAGGGTG TGGATCTGCT GCAGAACCTG 
ATAAAGTATT ATCACGCACA GTGCAATTTC TTTCAAGATG GTTTGAAAAC AGCTGATAAA 
TTGAAACAGT ACATTGAAAA GCTGGCTGCT GATTTGTATA ATATCAAACA GACCCAGGAC 
GAAGAAAAGA AACAGCTGAC CGCACTCCGA GACCTAATAA AGTCCTCGCT CCAACTCGAT 
CAGAAAGAAG ATTCCCAGAG CCGGCAGGGA GGCTACAGCA TGCACCAGCT GCAGGGCAAC 
AAGGAATACG GCAGCGAGAA GAAGGGCTAC CTGCTGAAGA AGAGTGACGG GATCCGGAAA 
GTGTGGCAGA GAAGGAAGTG CTCCGTCAAG AACGGGATCC TGACCATCTC CCACGCCACG 
TCCAACAGAC AGCCAGCCAA GCTGAACCTT CTCACTTGCC AGGTGAAGCC GAATGCCGAG 
GACAAGAAGT CTTTCGACCT GATATCACAT AACAGGACGT ATCACTTTCA GGCCGAAGAT 
GAGCAGGATT ATGTAGCGTG GATCTCGGTG CTGACGAACA GCAAAGAGGA GGCCCTCACC 
ATGGCCTTCC GGGGGGAACA GAGTGCTGGG GAGAGCAGCC TGGAGGAGCT GACGAAGGCC 
ATCATCGAGG ACGTGCAGCG GCTCCCGGGC AACGACGTCT GCTGCGACTG CGGCTCGGCA 
GAACCCACCT GGCTGTCCAC CAACTTGGGT ATCTTGACCT GTATAGAATG TTCCGGCATC 
CATAGAGAAA TGGGGGTTCA TATTTCTCGC ATCCAGTCTT TGGAACTAGA CAAATTAGGA 
ACTTCTGAAC TCTTGCTGGC CAAGAATGTA GGAAACAATA GTTTTAATGA TATTATGGAA 
GCAAATTTAC CCAGTCCCTC ACCAAAACCC ACCCCTTCAA GTGATATGAC CGTACGGAAG 
GAATATATCA CTGCAAAGTA TGTAGATCAT AGGTTTTCAC GGAAGACCTG TTCATCCTCA 
TCAGCTAAAC TGAACGAATT GCTTGAGGCC ATCAAATCCA GGGATTTACT TGCACTAATT 
CAAGTCTATG CAGAGGGGGT GGAGCTAATG GAACCGCTGC TGGAACCCGG ACAGGAGCTT 
GGGGAGACAG CCCTTCATCT TGCAGTCCGA ACCGCAGACC AGACATCTCT CCATTTGGTG 
GACTTCCTTG TACAAAACTG TGGGAACCTA GATAAGCAGA CGGCCCTGGG GAACACGGCC 
CTGCACTACT GTAGTATGTA CAGTAAACCA GAGTGTTTGA AGCTGCTGCT CAGGAGCAAG 
CCCACTGTGG ACGTCGTTAA TCAGGCTGGA GAGACCGCCC TGGACATAGC AAAGAGACTG 
AAAGCCACTC AGTGTGAAGA CCTGCTTTCC CAAGCTAAAT CTGGAAAGTT CAATCCACAC 
GTCCACGTGG AATATGAGTG GAATCTTCGA CAGGAGGAGA TGGATGAGAG CGATGACGAC 
CTGGATGACA AACCGAGCCC CATCAAGAAG GAGCGCTCCC CCCGACCGCA GAGCTTCTGC 
CACTCCTCCA GCATCTCCCC CCAGGACAAG CTCTCACTGC CGGGCTTCAG CACGCCAAGG 
GACAAGCAAC GACTCTCCTA CGGCGCCTTC ACCAACCAGA TCTTCGTCTC CACAAGCACA 
GACTCACCCA CGTCACCGAT CGCAGAGGCG CCCCCGCTGC CTCCCAGAAA CGCCACGAAA 
GGTCCACCTG GCCCACCTTC AACACTCCCT CTAAGCACCC AGACCTCTAG TGGCAGCTCC 
ACCCTGTCCA AGAAGCGGCC TCCTCCCCCA CCACCCGGAC ACAAGAGAAC CCTGTCTGAC 
CCTCCCAGCC CACTACCTCA CGGGCCCCCA AACAAAGGCG CAGTTCCTTG GGGTAACGAC 
GTGGGTCCCT CATCGTCCAG TAAGACCACG AACAAGTTCG AGGGCCTGTC CCAGCAGTCG 
AGCACCGGTT CTGCAAAGAC TGCACTTGGC CCAAGAGTTC TTCCTAAACT ACCTCAGAAA 
GTGGCACTAA GGAAAACAGA GACCAGCCAT CATCTCTCCC TCGACAAAGC CAACGTCCCA 
CCTGAGATCT TCCAGAAGTC GTCCCAGTTG ACAGAGTTAC CGCAGAAGCC GCCACCCGGG 
GACCTGCCCC CGAAGCCCAC GGAACTGGCT CCCAAACCCC CCATTGGAGA CTTACCACCT 
AAGCCAGGCG AGCTGCCCCC GAAGCCACAG CTGGGCGACC TGCCCCCCAA GCCCCAGCTC 
GCAGACTTGC CCCCCAAGCC CCAGGTGAAA GACCTGCCTC CCAAGCCACA ACTGGGGGAG 
CTGCTGGCAA AACCCCAGAC GGGAGACGCC TCGCCCAAGG CCCAGCCACC CCTGGAGCTC 
ACCCCCAAGT CACACCCGGC GGACCTGTCC CCGAACGTCC CCAAGCAGGC GTCTGAGGAC 
ACCAACGACC TCACGCCCAC CCTGCCAGAG ACACCTGTGC CGCTGCCCAG GAAGATCAAC 
ACGGGGAAGA GCAAGGTGAG GCGAGTGAAG ACCATCTACG ACTGCCAGGC GGACAACGAT 
GACGAGCTGA CTTTCATGGA GGGCGAGGTG ATCGTGGTCA CCGGGGAGGA GGACCAGGAG 
TGGTGGATTG GGCACATCGA GGGGCAGCCC GAGAGGAAGG GCGTCTTCCC AGTGTCCTTT 
GTCCACATCC TGTCGGACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024857199.1
CDS	95..3415
Translation	MPDQISVSEFIAETTEDYNSPTTSSFTTRLHNCRNTVTLLEEALDQDRTALQKVKKSVKAIYNSGQDHVQNEENYAQVLDKFGSNFLSRDNPDLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGVKGDLKKPFDKAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDQKEDSQSRQGGYSMHQLQGNKEYGSEKKGYLLKKSDGIRKVWQRRKCSVKNGILTISHATSNRQPAKLNLLTCQVKPNAEDKKSFDLISHNRTYHFQAEDEQDYVAWISVLTNSKEEALTMAFRGEQSAGESSLEELTKAIIEDVQRLPGNDVCCDCGSAEPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDKLGTSELLLAKNVGNNSFNDIMEANLPSPSPKPTPSSDMTVRKEYITAKYVDHRFSRKTCSSSSAKLNELLEAIKSRDLLALIQVYAEGVELMEPLLEPGQELGETALHLAVRTADQTSLHLVDFLVQNCGNLDKQTALGNTALHYCSMYSKPECLKLLLRSKPTVDVVNQAGETALDIAKRLKATQCEDLLSQAKSGKFNPHVHVEYEWNLRQEEMDESDDDLDDKPSPIKKERSPRPQSFCHSSSISPQDKLSLPGFSTPRDKQRLSYGAFTNQIFVSTSTDSPTSPIAEAPPLPPRNATKGPPGPPSTLPLSTQTSSGSSTLSKKRPPPPPPGHKRTLSDPPSPLPHGPPNKGAVPWGNDVGPSSSSKTTNKFEGLSQQSSTGSAKTALGPRVLPKLPQKVALRKTETSHHLSLDKANVPPEIFQKSSQLTELPQKPPPGDLPPKPTELAPKPPIGDLPPKPGELPPKPQLGDLPPKPQLADLPPKPQVKDLPPKPQLGELLAKPQTGDASPKAQPPLELTPKSHPADLSPNVPKQASEDTNDLTPTLPETPVPLPRKINTGKSKVRRVKTIYDCQADNDDELTFMEGEVIVVTGEEDQEWWIGHIEGQPERKGVFPVSFVHILSD

Target ORF information:

RefSeq Version	XM_025001431.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (ASAP1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025001431.1

ORF Insert Sequence:

ATGCCGGACC AGATCTCCGT CTCCGAGTTC ATCGCCGAGA CCACCGAGGA CTACAACTCG 
CCCACCACGT CCAGCTTCAC TACGCGGCTG CACAACTGCA GGAACACCGT CACGCTGCTG 
GAGGAGGCTC TAGACCAAGA TAGAACAGCC TTACAGAAAG TTAAGAAGTC TGTAAAAGCA 
ATATATAATT CCGGTCAAGA CCATGTACAA AATGAAGAAA ACTATGCACA AGTTCTTGAT 
AAGTTTGGGA GTAATTTTTT AAGTCGAGAC AACCCAGATC TTGGCACCGC TTTTGTCAAG 
TTTTCTACGC TTACAAAGGA ACTGTCCACA CTGCTGAAAA ATCTGCTCCA GGGCCTGAGC 
CACAATGTGA TCTTCACCTT GGATTCCTTG TTGAAAGGAG ACCTGAAGGG AGTCAAAGGC 
GATCTCAAGA AACCATTTGA CAAAGCTTGG AAAGATTATG AGACGAAGTT TACCAAAATT 
GAGAAGGAGA AGAGGGAGCA CGCCAAGCAG CACGGGATGA TCCGCACGGA GATCACCGGC 
GCCGAGATCG CGGAGGAAAT GGAGAAGGAG CGGCGCCTCT TCCAGCTCCA GATGTGCGAG 
TATCTCATTA AAGTTAATGA AATCAAGACC AAAAAGGGTG TGGATCTGCT GCAGAACCTG 
ATAAAGTATT ATCACGCACA GTGCAATTTC TTTCAAGATG GTTTGAAAAC AGCTGATAAA 
TTGAAACAGT ACATTGAAAA GCTGGCTGCT GATTTGTATA ATATCAAACA GACCCAGGAC 
GAAGAAAAGA AACAGCTGAC CGCACTCCGA GACCTAATAA AGTCCTCGCT CCAACTCGAT 
CAGAAAGAAG ATTCCCAGAG CCGGCAGGGA GGCTACAGCA TGCACCAGCT GCAGGGCAAC 
AAGGAATACG GCAGCGAGAA GAAGGGCTAC CTGCTGAAGA AGAGTGACGG GATCCGGAAA 
GTGTGGCAGA GAAGGAAGTG CTCCGTCAAG AACGGGATCC TGACCATCTC CCACGCCACG 
TCCAACAGAC AGCCAGCCAA GCTGAACCTT CTCACTTGCC AGGTGAAGCC GAATGCCGAG 
GACAAGAAGT CTTTCGACCT GATATCACAT AACAGGACGT ATCACTTTCA GGCCGAAGAT 
GAGCAGGATT ATGTAGCGTG GATCTCGGTG CTGACGAACA GCAAAGAGGA GGCCCTCACC 
ATGGCCTTCC GGGGGGAACA GAGTGCTGGG GAGAGCAGCC TGGAGGAGCT GACGAAGGCC 
ATCATCGAGG ACGTGCAGCG GCTCCCGGGC AACGACGTCT GCTGCGACTG CGGCTCGGCA 
GAACCCACCT GGCTGTCCAC CAACTTGGGT ATCTTGACCT GTATAGAATG TTCCGGCATC 
CATAGAGAAA TGGGGGTTCA TATTTCTCGC ATCCAGTCTT TGGAACTAGA CAAATTAGGA 
ACTTCTGAAC TCTTGCTGGC CAAGAATGTA GGAAACAATA GTTTTAATGA TATTATGGAA 
GCAAATTTAC CCAGTCCCTC ACCAAAACCC ACCCCTTCAA GTGATATGAC CGTACGGAAG 
GAATATATCA CTGCAAAGTA TGTAGATCAT AGGTTTTCAC GGAAGACCTG TTCATCCTCA 
TCAGCTAAAC TGAACGAATT GCTTGAGGCC ATCAAATCCA GGGATTTACT TGCACTAATT 
CAAGTCTATG CAGAGGGGGT GGAGCTAATG GAACCGCTGC TGGAACCCGG ACAGGAGCTT 
GGGGAGACAG CCCTTCATCT TGCAGTCCGA ACCGCAGACC AGACATCTCT CCATTTGGTG 
GACTTCCTTG TACAAAACTG TGGGAACCTA GATAAGCAGA CGGCCCTGGG GAACACGGCC 
CTGCACTACT GTAGTATGTA CAGTAAACCA GAGTGTTTGA AGCTGCTGCT CAGGAGCAAG 
CCCACTGTGG ACGTCGTTAA TCAGGCTGGA GAGACCGCCC TGGACATAGC AAAGAGACTG 
AAAGCCACTC AGTGTGAAGA CCTGCTTTCC CAAGCTAAAT CTGGAAAGTT CAATCCACAC 
GTCCACGTGG AATATGAGTG GAATCTTCGA CAGGAGGAGA TGGATGAGAG CGATGACGAC 
CTGGATGACA AACCGAGCCC CATCAAGAAG GAGCGCTCCC CCCGACCGCA GAGCTTCTGC 
CACTCCTCCA GCATCTCCCC CCAGGACAAG CTCTCACTGC CGGGCTTCAG CACGCCAAGG 
GACAAGCAAC GACTCTCCTA CGGCGCCTTC ACCAACCAGA TCTTCGTCTC CACAAGCACA 
GACTCACCCA CGTCACCGAT CGCAGAGGCG CCCCCGCTGC CTCCCAGAAA CGCCACGAAA 
GGTCCACCTG GCCCACCTTC AACACTCCCT CTAAGCACCC AGACCTCTAG TGGCAGCTCC 
ACCCTGTCCA AGAAGCGGCC TCCTCCCCCA CCACCCGGAC ACAAGAGAAC CCTGTCTGAC 
CCTCCCAGCC CACTACCTCA CGGGCCCCCA AACAAAGGCG CAGTTCCTTG GGGTAACGAC 
GTGGGTCCCT CATCGTCCAG TAAGACCACG AACAAGTTCG AGGGCCTGTC CCAGCAGTCG 
AGCACCGGTT CTGCAAAGAC TGCACTTGGC CCAAGAGTTC TTCCTAAACT ACCTCAGAAA 
GTGGCACTAA GGAAAACAGA GACCAGCCAT CATCTCTCCC TCGACAAAGC CAACGTCCCA 
CCTGAGATCT TCCAGAAGTC GTCCCAGTTG ACAGAGTTAC CGCAGAAGCC GCCACCCGGG 
GACCTGCCCC CGAAGCCCAC GGAACTGGCT CCCAAACCCC CCATTGGAGA CTTACCACCT 
AAGCCAGGCG AGCTGCCCCC GAAGCCACAG CTGGGCGACC TGCCCCCCAA GCCCCAGCTC 
GCAGACTTGC CCCCCAAGCC CCAGGTGAAA GACCTGCCTC CCAAGCCACA ACTGGGGGAG 
CTGCTGGCAA AACCCCAGAC GGGAGACGCC TCGCCCAAGG CCCAGCCACC CCTGGAGCTC 
ACCCCCAAGT CACACCCGGC GGACCTGTCC CCGAACGTCC CCAAGCAGGC GTCTGAGGAC 
ACCAACGACC TCACGCCCAC CCTGCCAGAG ACACCTGTGC CGCTGCCCAG GAAGATCAAC 
ACGGGGAAGA GCAAGGTGAG GCGAGTGAAG ACCATCTACG ACTGCCAGGC GGACAACGAT 
GACGAGCTGA CTTTCATGGA GGGCGAGGTG ATCGTGGTCA CCGGGGAGGA GGACCAGGAG 
TGGTGGATTG GGCACATCGA GGGGCAGCCC GAGAGGAAGG GCGTCTTCCC AGTGTCCTTT 
GTCCACATCC TGTCGGACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASAP1 ( XM_025001432.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024857200.1 Bos taurus ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (ASAP1), transcript variant X3, mRNA.

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CloneID	OBa222205
Clone ID Related Accession (Same CDS sequence)	XM_025001432.1
Accession Version	XM_025001432.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATCTT CAGCCTCCAG GCTCTCCAGT TTTTCATCAA GAGATTCGCT ATGGAATCGG 
ATGCCGGACC AGATCTCCGT CTCCGAGTTC ATCGCCGAGA CCACCGAGGA CTACAACTCG 
CCCACCACGT CCAGCTTCAC TACGCGGCTG CACAACTGCA GGAACACCGT CACGCTGCTG 
GAGGAGGCTC TAGACCAAGA TAGAACAGCC TTACAGAAAG TTAAGAAGTC TGTAAAAGCA 
ATATATAATT CCGGTCAAGA CCATGTACAA AATGAAGAAA ACTATGCACA AGTTCTTGAT 
AAGTTTGGGA GTAATTTTTT AAGTCGAGAC AACCCAGATC TTGGCACCGC TTTTGTCAAG 
TTTTCTACGC TTACAAAGGA ACTGTCCACA CTGCTGAAAA ATCTGCTCCA GGGCCTGAGC 
CACAATGTGA TCTTCACCTT GGATTCCTTG TTGAAAGGAG ACCTGAAGGG AGTCAAAGGC 
GATCTCAAGA AACCATTTGA CAAAGCTTGG AAAGATTATG AGACGAAGTT TACCAAAATT 
GAGAAGGAGA AGAGGGAGCA CGCCAAGCAG CACGGGATGA TCCGCACGGA GATCACCGGC 
GCCGAGATCG CGGAGGAAAT GGAGAAGGAG CGGCGCCTCT TCCAGCTCCA GATGTGCGAG 
TATCTCATTA AAGTTAATGA AATCAAGACC AAAAAGGGTG TGGATCTGCT GCAGAACCTG 
ATAAAGTATT ATCACGCACA GTGCAATTTC TTTCAAGATG GTTTGAAAAC AGCTGATAAA 
TTGAAACAGT ACATTGAAAA GCTGGCTGCT GATTTGTATA ATATCAAACA GACCCAGGAC 
GAAGAAAAGA AACAGCTGAC CGCACTCCGA GACCTAATAA AGTCCTCGCT CCAACTCGAT 
CAGAAAGAAG ATTCCCAGAG CCGGCAGGGA GGCTACAGCA TGCACCAGCT GCAGGGCAAC 
AAGGAATACG GCAGCGAGAA GAAGGGCTAC CTGCTGAAGA AGAGTGACGG GATCCGGAAA 
GTGTGGCAGA GAAGGAAGTG CTCCGTCAAG AACGGGATCC TGACCATCTC CCACGCCACG 
TCCAACAGAC AGCCAGCCAA GCTGAACCTT CTCACTTGCC AGGTGAAGCC GAATGCCGAG 
GACAAGAAGT CTTTCGACCT GATATCACAT AACAGGACGT ATCACTTTCA GGCCGAAGAT 
GAGCAGGATT ATGTAGCGTG GATCTCGGTG CTGACGAACA GCAAAGAGGA GGCCCTCACC 
ATGGCCTTCC GGGGGGAACA GAGTGCTGGG GAGAGCAGCC TGGAGGAGCT GACGAAGGCC 
ATCATCGAGG ACGTGCAGCG GCTCCCGGGC AACGACGTCT GCTGCGACTG CGGCTCGGCA 
GAACCCACCT GGCTGTCCAC CAACTTGGGT ATCTTGACCT GTATAGAATG TTCCGGCATC 
CATAGAGAAA TGGGGGTTCA TATTTCTCGC ATCCAGTCTT TGGAACTAGA CAAATTAGGA 
ACTTCTGAAC TCTTGCTGGC CAAGAATGTA GGAAACAATA GTTTTAATGA TATTATGGAA 
GCAAATTTAC CCAGTCCCTC ACCAAAACCC ACCCCTTCAA GTGATATGAC CGTACGGAAG 
GAATATATCA CTGCAAAGTA TGTAGATCAT AGGTTTTCAC GGAAGACCTG TTCATCCTCA 
TCAGCTAAAC TGAACGAATT GCTTGAGGCC ATCAAATCCA GGGATTTACT TGCACTAATT 
CAAGTCTATG CAGAGGGGGT GGAGCTAATG GAACCGCTGC TGGAACCCGG ACAGGAGCTT 
GGGGAGACAG CCCTTCATCT TGCAGTCCGA ACCGCAGACC AGACATCTCT CCATTTGGTG 
GACTTCCTTG TACAAAACTG TGGGAACCTA GATAAGCAGA CGGCCCTGGG GAACACGGCC 
CTGCACTACT GTAGTATGTA CAGTAAACCA GAGTGTTTGA AGCTGCTGCT CAGGAGCAAG 
CCCACTGTGG ACGTCGTTAA TCAGGCTGGA GAGACCGCCC TGGACATAGC AAAGAGACTG 
AAAGCCACTC AGTGTGAAGA CCTGCTTTCC CAAGCTAAAT CTGGAAAGTT CAATCCACAC 
GTCCACGTGG AATATGAGTG GAATCTTCGA CAGGAGGAGA TGGATGAGAG CGATGACGAC 
CTGGATGACA AACCGAGCCC CATCAAGAAG GAGCGCTCCC CCCGACCGCA GAGCTTCTGC 
CACTCCTCCA GCATCTCCCC CCAGGACAAG CTCTCACTGC CGGGCTTCAG CACGCCAAGG 
GACAAGCAAC GACTCTCCTA CGGCGCCTTC ACCAACCAGA TCTTCGTCTC CACAAGCACA 
GACTCACCCA CGTCACCGAT CGCAGAGGCG CCCCCGCTGC CTCCCAGAAA CGCCACGAAA 
GGTAACGACG TGGGTCCCTC ATCGTCCAGT AAGACCACGA ACAAGTTCGA GGGCCTGTCC 
CAGCAGTCGA GCACCGGTTC TGCAAAGACT GCACTTGGCC CAAGAGTTCT TCCTAAACTA 
CCTCAGAAAG TGGCACTAAG GAAAACAGAG ACCAGCCATC ATCTCTCCCT CGACAAAGCC 
AACGTCCCAC CTGAGATCTT CCAGAAGTCG TCCCAGTTGA CAGAGTTACC GCAGAAGCCG 
CCACCCGGGG ACCTGCCCCC GAAGCCCACG GAACTGGCTC CCAAACCCCC CATTGGAGAC 
TTACCACCTA AGCCAGGCGA GCTGCCCCCG AAGCCACAGC TGGGCGACCT GCCCCCCAAG 
CCCCAGCTCG CAGACTTGCC CCCCAAGCCC CAGGTGAAAG ACCTGCCTCC CAAGCCACAA 
CTGGGGGAGC TGCTGGCAAA ACCCCAGACG GGAGACGCCT CGCCCAAGGC CCAGCCACCC 
CTGGAGCTCA CCCCCAAGTC ACACCCGGCG GACCTGTCCC CGAACGTCCC CAAGCAGGCG 
TCTGAGGACA CCAACGACCT CACGCCCACC CTGCCAGAGA CACCTGTGCC GCTGCCCAGG 
AAGATCAACA CGGGGAAGAG CAAGGTGAGG CGAGTGAAGA CCATCTACGA CTGCCAGGCG 
GACAACGATG ACGAGCTGAC TTTCATGGAG GGCGAGGTGA TCGTGGTCAC CGGGGAGGAG 
GACCAGGAGT GGTGGATTGG GCACATCGAG GGGCAGCCCG AGAGGAAGGG CGTCTTCCCA 
GTGTCCTTTG TCCACATCCT GTCGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024857200.1
CDS	209..3418
Translation	MRSSASRLSSFSSRDSLWNRMPDQISVSEFIAETTEDYNSPTTSSFTTRLHNCRNTVTLLEEALDQDRTALQKVKKSVKAIYNSGQDHVQNEENYAQVLDKFGSNFLSRDNPDLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGVKGDLKKPFDKAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDQKEDSQSRQGGYSMHQLQGNKEYGSEKKGYLLKKSDGIRKVWQRRKCSVKNGILTISHATSNRQPAKLNLLTCQVKPNAEDKKSFDLISHNRTYHFQAEDEQDYVAWISVLTNSKEEALTMAFRGEQSAGESSLEELTKAIIEDVQRLPGNDVCCDCGSAEPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDKLGTSELLLAKNVGNNSFNDIMEANLPSPSPKPTPSSDMTVRKEYITAKYVDHRFSRKTCSSSSAKLNELLEAIKSRDLLALIQVYAEGVELMEPLLEPGQELGETALHLAVRTADQTSLHLVDFLVQNCGNLDKQTALGNTALHYCSMYSKPECLKLLLRSKPTVDVVNQAGETALDIAKRLKATQCEDLLSQAKSGKFNPHVHVEYEWNLRQEEMDESDDDLDDKPSPIKKERSPRPQSFCHSSSISPQDKLSLPGFSTPRDKQRLSYGAFTNQIFVSTSTDSPTSPIAEAPPLPPRNATKGNDVGPSSSSKTTNKFEGLSQQSSTGSAKTALGPRVLPKLPQKVALRKTETSHHLSLDKANVPPEIFQKSSQLTELPQKPPPGDLPPKPTELAPKPPIGDLPPKPGELPPKPQLGDLPPKPQLADLPPKPQVKDLPPKPQLGELLAKPQTGDASPKAQPPLELTPKSHPADLSPNVPKQASEDTNDLTPTLPETPVPLPRKINTGKSKVRRVKTIYDCQADNDDELTFMEGEVIVVTGEEDQEWWIGHIEGQPERKGVFPVSFVHILSD

Target ORF information:

RefSeq Version	XM_025001432.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (ASAP1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025001432.1

ORF Insert Sequence:

ATGAGATCTT CAGCCTCCAG GCTCTCCAGT TTTTCATCAA GAGATTCGCT ATGGAATCGG 
ATGCCGGACC AGATCTCCGT CTCCGAGTTC ATCGCCGAGA CCACCGAGGA CTACAACTCG 
CCCACCACGT CCAGCTTCAC TACGCGGCTG CACAACTGCA GGAACACCGT CACGCTGCTG 
GAGGAGGCTC TAGACCAAGA TAGAACAGCC TTACAGAAAG TTAAGAAGTC TGTAAAAGCA 
ATATATAATT CCGGTCAAGA CCATGTACAA AATGAAGAAA ACTATGCACA AGTTCTTGAT 
AAGTTTGGGA GTAATTTTTT AAGTCGAGAC AACCCAGATC TTGGCACCGC TTTTGTCAAG 
TTTTCTACGC TTACAAAGGA ACTGTCCACA CTGCTGAAAA ATCTGCTCCA GGGCCTGAGC 
CACAATGTGA TCTTCACCTT GGATTCCTTG TTGAAAGGAG ACCTGAAGGG AGTCAAAGGC 
GATCTCAAGA AACCATTTGA CAAAGCTTGG AAAGATTATG AGACGAAGTT TACCAAAATT 
GAGAAGGAGA AGAGGGAGCA CGCCAAGCAG CACGGGATGA TCCGCACGGA GATCACCGGC 
GCCGAGATCG CGGAGGAAAT GGAGAAGGAG CGGCGCCTCT TCCAGCTCCA GATGTGCGAG 
TATCTCATTA AAGTTAATGA AATCAAGACC AAAAAGGGTG TGGATCTGCT GCAGAACCTG 
ATAAAGTATT ATCACGCACA GTGCAATTTC TTTCAAGATG GTTTGAAAAC AGCTGATAAA 
TTGAAACAGT ACATTGAAAA GCTGGCTGCT GATTTGTATA ATATCAAACA GACCCAGGAC 
GAAGAAAAGA AACAGCTGAC CGCACTCCGA GACCTAATAA AGTCCTCGCT CCAACTCGAT 
CAGAAAGAAG ATTCCCAGAG CCGGCAGGGA GGCTACAGCA TGCACCAGCT GCAGGGCAAC 
AAGGAATACG GCAGCGAGAA GAAGGGCTAC CTGCTGAAGA AGAGTGACGG GATCCGGAAA 
GTGTGGCAGA GAAGGAAGTG CTCCGTCAAG AACGGGATCC TGACCATCTC CCACGCCACG 
TCCAACAGAC AGCCAGCCAA GCTGAACCTT CTCACTTGCC AGGTGAAGCC GAATGCCGAG 
GACAAGAAGT CTTTCGACCT GATATCACAT AACAGGACGT ATCACTTTCA GGCCGAAGAT 
GAGCAGGATT ATGTAGCGTG GATCTCGGTG CTGACGAACA GCAAAGAGGA GGCCCTCACC 
ATGGCCTTCC GGGGGGAACA GAGTGCTGGG GAGAGCAGCC TGGAGGAGCT GACGAAGGCC 
ATCATCGAGG ACGTGCAGCG GCTCCCGGGC AACGACGTCT GCTGCGACTG CGGCTCGGCA 
GAACCCACCT GGCTGTCCAC CAACTTGGGT ATCTTGACCT GTATAGAATG TTCCGGCATC 
CATAGAGAAA TGGGGGTTCA TATTTCTCGC ATCCAGTCTT TGGAACTAGA CAAATTAGGA 
ACTTCTGAAC TCTTGCTGGC CAAGAATGTA GGAAACAATA GTTTTAATGA TATTATGGAA 
GCAAATTTAC CCAGTCCCTC ACCAAAACCC ACCCCTTCAA GTGATATGAC CGTACGGAAG 
GAATATATCA CTGCAAAGTA TGTAGATCAT AGGTTTTCAC GGAAGACCTG TTCATCCTCA 
TCAGCTAAAC TGAACGAATT GCTTGAGGCC ATCAAATCCA GGGATTTACT TGCACTAATT 
CAAGTCTATG CAGAGGGGGT GGAGCTAATG GAACCGCTGC TGGAACCCGG ACAGGAGCTT 
GGGGAGACAG CCCTTCATCT TGCAGTCCGA ACCGCAGACC AGACATCTCT CCATTTGGTG 
GACTTCCTTG TACAAAACTG TGGGAACCTA GATAAGCAGA CGGCCCTGGG GAACACGGCC 
CTGCACTACT GTAGTATGTA CAGTAAACCA GAGTGTTTGA AGCTGCTGCT CAGGAGCAAG 
CCCACTGTGG ACGTCGTTAA TCAGGCTGGA GAGACCGCCC TGGACATAGC AAAGAGACTG 
AAAGCCACTC AGTGTGAAGA CCTGCTTTCC CAAGCTAAAT CTGGAAAGTT CAATCCACAC 
GTCCACGTGG AATATGAGTG GAATCTTCGA CAGGAGGAGA TGGATGAGAG CGATGACGAC 
CTGGATGACA AACCGAGCCC CATCAAGAAG GAGCGCTCCC CCCGACCGCA GAGCTTCTGC 
CACTCCTCCA GCATCTCCCC CCAGGACAAG CTCTCACTGC CGGGCTTCAG CACGCCAAGG 
GACAAGCAAC GACTCTCCTA CGGCGCCTTC ACCAACCAGA TCTTCGTCTC CACAAGCACA 
GACTCACCCA CGTCACCGAT CGCAGAGGCG CCCCCGCTGC CTCCCAGAAA CGCCACGAAA 
GGTAACGACG TGGGTCCCTC ATCGTCCAGT AAGACCACGA ACAAGTTCGA GGGCCTGTCC 
CAGCAGTCGA GCACCGGTTC TGCAAAGACT GCACTTGGCC CAAGAGTTCT TCCTAAACTA 
CCTCAGAAAG TGGCACTAAG GAAAACAGAG ACCAGCCATC ATCTCTCCCT CGACAAAGCC 
AACGTCCCAC CTGAGATCTT CCAGAAGTCG TCCCAGTTGA CAGAGTTACC GCAGAAGCCG 
CCACCCGGGG ACCTGCCCCC GAAGCCCACG GAACTGGCTC CCAAACCCCC CATTGGAGAC 
TTACCACCTA AGCCAGGCGA GCTGCCCCCG AAGCCACAGC TGGGCGACCT GCCCCCCAAG 
CCCCAGCTCG CAGACTTGCC CCCCAAGCCC CAGGTGAAAG ACCTGCCTCC CAAGCCACAA 
CTGGGGGAGC TGCTGGCAAA ACCCCAGACG GGAGACGCCT CGCCCAAGGC CCAGCCACCC 
CTGGAGCTCA CCCCCAAGTC ACACCCGGCG GACCTGTCCC CGAACGTCCC CAAGCAGGCG 
TCTGAGGACA CCAACGACCT CACGCCCACC CTGCCAGAGA CACCTGTGCC GCTGCCCAGG 
AAGATCAACA CGGGGAAGAG CAAGGTGAGG CGAGTGAAGA CCATCTACGA CTGCCAGGCG 
GACAACGATG ACGAGCTGAC TTTCATGGAG GGCGAGGTGA TCGTGGTCAC CGGGGAGGAG 
GACCAGGAGT GGTGGATTGG GCACATCGAG GGGCAGCCCG AGAGGAAGGG CGTCTTCCCA 
GTGTCCTTTG TCCACATCCT GTCGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASAP1 ( NM_175821.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_787015.1 Bos taurus ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (ASAP1), mRNA.

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CloneID	OBa41716
Clone ID Related Accession (Same CDS sequence)	NM_175821.2
Accession Version	NM_175821.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3390bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-21
Organism	Bos taurus(cattle)
Product	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF112886.1. On Jun 3, 2003 this sequence version replaced NM_175821.1. ##Evidence-Data-START## Transcript exon combination :: AF112886.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02822092, SAMN02822093 [ECO:0000348] ##Evidence-Data-END##

ATGAGATCTT CAGCCTCCAG GCTCTCCAGT TTTTCATCAA GAGATTCGCT ATGGAATCGG 
ATGCCGGACC AGATCTCCGT CTCCGAGTTC ATCGCCGAGA CCACCGAGGA CTACAACTCG 
CCCACCACGT CCAGCTTCAC TACGCGGCTG CACAACTGCA GGAACACCGT CACGCTGCTG 
GAGGAGGCTC TAGACCAAGA TAGAACAGCC TTACAGAAAG TTAAGAAGTC TGTAAAAGCA 
ATATACAATT CCGGTCAAGA CCATGTACAA AATGAAGAAA ACTATGCGCA AGTTCTTGAT 
AAGTTTGGGA GTAATTTTTT AAGTCGAGAC AACCCAGATC TTGGCACCGC TTTTGTCAAG 
TTTTCTACGC TTACAAAGGA ACTGTCCACA CTGCTGAAAA ATCTGCTCCA GGGCCTGAGC 
CACAATGTGA TCTTCACCTT GGATTCCTTG TTGAAAGGAG ACCTGAAGGG AGTCAAAGGC 
GATCTCAAGA AACCATTTGA CAAAGCTTGG AAAGATTATG AGACGAAGTT TACCAAAATT 
GAGAAGGAGA AGAGGGAGCA CGCCAAGCAG CACGGGATGA TCCGCACGGA GATCACCGGC 
GCCGAGATCG CGGAGGAAAT GGAAAAGGAG CGGCGCCTCT TCCAGCTCCA GATGTGCGAG 
TATCTCATTA AAGTTAATGA AATCAAGACC AAAAAGGGTG TGGATCTGCT GCAGAACCTG 
ATAAAGTATT ATCACGCACA GTGCAATTTC TTTCAAGATG GTTTGAAAAC AGCTGATAAA 
TTGAAACAGT ACATTGAAAA GCTGGCTGCT GATTTGTATA ATATCAAACA GACCCAGGAC 
GAAGAAAAGA AACAGCTGAC CGCACTCCGA GACCTAATAA AGTCCTCGCT CCAACTCGAT 
CAGAAGGAGT CTAGGAGAGA TTCCCAGAGC CGGCAGGGAG GCTACAGCAT GCACCAGCTG 
CAGGGCAACA AGGAATACGG CAGCGAGAAG AAGGGCTACC TGCTGAAGAA GAGTGACGGG 
ATCCGGAAAG TGTGGCAGAG AAGGAAGTGC TCCGTCAAGA ACGGGATCCT GACCATCTCC 
CACGCCACGT CCAACAGACA GCCAGCCAAG CTGAACCTTC TCACTTGCCA GGTGAAGCCG 
AATGCCGAGG ACAAGAAGTC TTTTGACCTG ATATCACATA ACAGGACGTA TCACTTTCAG 
GCCGAAGATG AGCAGGATTA TGTAGCGTGG ATCTCGGTGC TGACAAACAG CAAAGAGGAG 
GCCCTCACCA TGGCCTTCCG GGGGGAACAG AGTGCTGGGG AGAGCAGCCT GGAGGAGCTG 
ACGAAGGCCA TCATCGAGGA CGTGCAGCGG CTCCCGGGCA ACGACGTCTG CTGCGACTGC 
GGCTCGGCAG AACCCACCTG GCTGTCCACC AACTTGGGCA TCTTGACCTG TATAGAATGT 
TCCGGCATCC ATAGAGAAAT GGGGGTTCAT ATTTCTCGCA TCCAGTCTTT GGAACTAGAC 
AAATTAGGAA CTTCTGAACT CTTGCTGGCC AAGAATGTAG GAAACAATAG TTTTAATGAT 
ATTATGGAAG CAAATTTACC CAGTCCCTCA CCAAAACCCA CCCCTTCAAG TGATATGACT 
GTACGGAAGG AATATATCAC TGCAAAGTAT GTAGATCATA GGTTTTCACG GAAGACCTGT 
TCATCGTCAT CAGCTAAACT GAACGAATTG CTTGAGGCCA TCAAATCCAG GGATTTACTT 
GCACTAATTC AAGTCTATGC AGAGGGGGTG GAGCTAATGG AACCGCTGCT GGAACCCGGA 
CAGGAGCTTG GGGAGACAGC CCTTCATCTT GCAGTCCGAA CCGCAGACCA GACATCTCTC 
CATTTGGTGG ACTTCCTTGT ACAAAACTGT GGGAACCTAG ATAAGCAGAC GGCCCTGGGG 
AACACGGCCC TGCACTACTG TAGTATGTAC AGTAAACCAG AGTGTTTGAA GCTGCTGCTC 
AGGAGCAAGC CCACTGTGGA CGTCGTTAAT CAGGCTGGAG AGACCGCCCT GGACATAGCA 
AAGAGACTGA AAGCCACTCA GTGTGAAGAC CTGCTTTCCC AAGCTAAATC TGGAAAGTTC 
AATCCACACG TCCACGTGGA ATATGAGTGG AATCTTCGAC AGGAGGAGAT GGATGAGAGC 
GATGACGACC TGGATGACAA ACCGAGCCCC ATCAAGAAGG AGCGCTCCCC CCGACCGCAG 
AGCTTCTGCC ACTCCTCCAG CATCTCCCCC CAGGACAAGC TCTCACTGCC GGGCTTCAGC 
ACGCCAAGGG ACAAGCAACG ACTCTCCTAC GGCGCCTTCA CCAACCAGAT CTTCGTCTCC 
ACAAGCACAG ACTCACCCAC GTCACCGATC GCAGAGGCGC CCCCGCTGCC TCCCAGAAAC 
GCCACGAAAG GTCCACCTGG CCCACCTTCA ACACTCCCTC TAAGCACCCA GACCTCTAGT 
GGCAGCTCCA CCCTGTCCAA GAAGCGGTCT CCTCCCCCAC CACCCGGACA CAAGAGAACC 
CTGTCTGACC CTCCCAGCCC ACTACCTCAC GGGCCCCCAA ACAAAGGCGC AGTTCCTTGG 
GGTAACGACG TGGGTCCCTC ATCGTCCAGT AAGACCACGA ACAAGTTCGA GGGCCTGTCC 
CAGCAGTCGA GCACCGGTTC TGCAAAGACT GCACTTGGCC CAAGAGTTCT TCCTAAACTA 
CCTCAGAAAG TGGCACTAAG GAAAACAGAG ACCAGCCATC ATCTCTCCCT CGACAAAGCC 
AACGTCCCAC CTGAGATCTT CCAGAAGTCG TCCCAGTTGA CAGAGTTACC GCAGAAGCCG 
CCACCCGGGG ACCTGCCCCC GAAGCCCACG GAACTGGCTC CCAAACCCCC CATTGGAGAC 
TTACCACCTA AGCCAGGCGA GCTGCCCCCG AAGCCACAGC TGGGCGACCT GCCCCCCAAG 
CCCCAGCTCG CAGACTTGCC CCCCAAGCCC CAGGTGAAAG ACCTGCCTCC CAAGCCACAA 
CTGGGGGAGC TGCTGGCAAA ACCCCAGACG GGAGACGCCT CGCCCAAGGC CCAGCCACCC 
CTGGAGCTCA CCCCCAAGTC ACACCCGGCG GACCTGTCCC CGAACGTCCC CAAGCAGGCG 
TCTGAGGACA CCAACGACCT CACGCCCACC CTGCCAGAGA CACCCGTGCC TCTGCCCAGG 
AAGATCAACA CGGGGAAGAG CAAGGTGAGG CGAGTGAAGA CCATCTACGA CTGCCAGGCG 
GACAACGATG ACGAGCTGAC TTTCATGGAG GGCGAGGTGA TCGTGGTCAC CGGGGAGGAG 
GACCAGGAGT GGTGGATTGG GCACATCGAG GGGCAGCCCG AGAGGAAGGG CGTCTTCCCA 
GTGTCCTTTG TCCACATCCT GTCGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_787015.1
CDS	209..3598
Translation	MRSSASRLSSFSSRDSLWNRMPDQISVSEFIAETTEDYNSPTTSSFTTRLHNCRNTVTLLEEALDQDRTALQKVKKSVKAIYNSGQDHVQNEENYAQVLDKFGSNFLSRDNPDLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGVKGDLKKPFDKAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDQKESRRDSQSRQGGYSMHQLQGNKEYGSEKKGYLLKKSDGIRKVWQRRKCSVKNGILTISHATSNRQPAKLNLLTCQVKPNAEDKKSFDLISHNRTYHFQAEDEQDYVAWISVLTNSKEEALTMAFRGEQSAGESSLEELTKAIIEDVQRLPGNDVCCDCGSAEPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDKLGTSELLLAKNVGNNSFNDIMEANLPSPSPKPTPSSDMTVRKEYITAKYVDHRFSRKTCSSSSAKLNELLEAIKSRDLLALIQVYAEGVELMEPLLEPGQELGETALHLAVRTADQTSLHLVDFLVQNCGNLDKQTALGNTALHYCSMYSKPECLKLLLRSKPTVDVVNQAGETALDIAKRLKATQCEDLLSQAKSGKFNPHVHVEYEWNLRQEEMDESDDDLDDKPSPIKKERSPRPQSFCHSSSISPQDKLSLPGFSTPRDKQRLSYGAFTNQIFVSTSTDSPTSPIAEAPPLPPRNATKGPPGPPSTLPLSTQTSSGSSTLSKKRSPPPPPGHKRTLSDPPSPLPHGPPNKGAVPWGNDVGPSSSSKTTNKFEGLSQQSSTGSAKTALGPRVLPKLPQKVALRKTETSHHLSLDKANVPPEIFQKSSQLTELPQKPPPGDLPPKPTELAPKPPIGDLPPKPGELPPKPQLGDLPPKPQLADLPPKPQVKDLPPKPQLGELLAKPQTGDASPKAQPPLELTPKSHPADLSPNVPKQASEDTNDLTPTLPETPVPLPRKINTGKSKVRRVKTIYDCQADNDDELTFMEGEVIVVTGEEDQEWWIGHIEGQPERKGVFPVSFVHILSD

Target ORF information:

RefSeq Version	NM_175821.2
Organism	Bos taurus(cattle)
Definition	Bos taurus ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (ASAP1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175821.2

ORF Insert Sequence:

ATGAGATCTT CAGCCTCCAG GCTCTCCAGT TTTTCATCAA GAGATTCGCT ATGGAATCGG 
ATGCCGGACC AGATCTCCGT CTCCGAGTTC ATCGCCGAGA CCACCGAGGA CTACAACTCG 
CCCACCACGT CCAGCTTCAC TACGCGGCTG CACAACTGCA GGAACACCGT CACGCTGCTG 
GAGGAGGCTC TAGACCAAGA TAGAACAGCC TTACAGAAAG TTAAGAAGTC TGTAAAAGCA 
ATATACAATT CCGGTCAAGA CCATGTACAA AATGAAGAAA ACTATGCGCA AGTTCTTGAT 
AAGTTTGGGA GTAATTTTTT AAGTCGAGAC AACCCAGATC TTGGCACCGC TTTTGTCAAG 
TTTTCTACGC TTACAAAGGA ACTGTCCACA CTGCTGAAAA ATCTGCTCCA GGGCCTGAGC 
CACAATGTGA TCTTCACCTT GGATTCCTTG TTGAAAGGAG ACCTGAAGGG AGTCAAAGGC 
GATCTCAAGA AACCATTTGA CAAAGCTTGG AAAGATTATG AGACGAAGTT TACCAAAATT 
GAGAAGGAGA AGAGGGAGCA CGCCAAGCAG CACGGGATGA TCCGCACGGA GATCACCGGC 
GCCGAGATCG CGGAGGAAAT GGAAAAGGAG CGGCGCCTCT TCCAGCTCCA GATGTGCGAG 
TATCTCATTA AAGTTAATGA AATCAAGACC AAAAAGGGTG TGGATCTGCT GCAGAACCTG 
ATAAAGTATT ATCACGCACA GTGCAATTTC TTTCAAGATG GTTTGAAAAC AGCTGATAAA 
TTGAAACAGT ACATTGAAAA GCTGGCTGCT GATTTGTATA ATATCAAACA GACCCAGGAC 
GAAGAAAAGA AACAGCTGAC CGCACTCCGA GACCTAATAA AGTCCTCGCT CCAACTCGAT 
CAGAAGGAGT CTAGGAGAGA TTCCCAGAGC CGGCAGGGAG GCTACAGCAT GCACCAGCTG 
CAGGGCAACA AGGAATACGG CAGCGAGAAG AAGGGCTACC TGCTGAAGAA GAGTGACGGG 
ATCCGGAAAG TGTGGCAGAG AAGGAAGTGC TCCGTCAAGA ACGGGATCCT GACCATCTCC 
CACGCCACGT CCAACAGACA GCCAGCCAAG CTGAACCTTC TCACTTGCCA GGTGAAGCCG 
AATGCCGAGG ACAAGAAGTC TTTTGACCTG ATATCACATA ACAGGACGTA TCACTTTCAG 
GCCGAAGATG AGCAGGATTA TGTAGCGTGG ATCTCGGTGC TGACAAACAG CAAAGAGGAG 
GCCCTCACCA TGGCCTTCCG GGGGGAACAG AGTGCTGGGG AGAGCAGCCT GGAGGAGCTG 
ACGAAGGCCA TCATCGAGGA CGTGCAGCGG CTCCCGGGCA ACGACGTCTG CTGCGACTGC 
GGCTCGGCAG AACCCACCTG GCTGTCCACC AACTTGGGCA TCTTGACCTG TATAGAATGT 
TCCGGCATCC ATAGAGAAAT GGGGGTTCAT ATTTCTCGCA TCCAGTCTTT GGAACTAGAC 
AAATTAGGAA CTTCTGAACT CTTGCTGGCC AAGAATGTAG GAAACAATAG TTTTAATGAT 
ATTATGGAAG CAAATTTACC CAGTCCCTCA CCAAAACCCA CCCCTTCAAG TGATATGACT 
GTACGGAAGG AATATATCAC TGCAAAGTAT GTAGATCATA GGTTTTCACG GAAGACCTGT 
TCATCGTCAT CAGCTAAACT GAACGAATTG CTTGAGGCCA TCAAATCCAG GGATTTACTT 
GCACTAATTC AAGTCTATGC AGAGGGGGTG GAGCTAATGG AACCGCTGCT GGAACCCGGA 
CAGGAGCTTG GGGAGACAGC CCTTCATCTT GCAGTCCGAA CCGCAGACCA GACATCTCTC 
CATTTGGTGG ACTTCCTTGT ACAAAACTGT GGGAACCTAG ATAAGCAGAC GGCCCTGGGG 
AACACGGCCC TGCACTACTG TAGTATGTAC AGTAAACCAG AGTGTTTGAA GCTGCTGCTC 
AGGAGCAAGC CCACTGTGGA CGTCGTTAAT CAGGCTGGAG AGACCGCCCT GGACATAGCA 
AAGAGACTGA AAGCCACTCA GTGTGAAGAC CTGCTTTCCC AAGCTAAATC TGGAAAGTTC 
AATCCACACG TCCACGTGGA ATATGAGTGG AATCTTCGAC AGGAGGAGAT GGATGAGAGC 
GATGACGACC TGGATGACAA ACCGAGCCCC ATCAAGAAGG AGCGCTCCCC CCGACCGCAG 
AGCTTCTGCC ACTCCTCCAG CATCTCCCCC CAGGACAAGC TCTCACTGCC GGGCTTCAGC 
ACGCCAAGGG ACAAGCAACG ACTCTCCTAC GGCGCCTTCA CCAACCAGAT CTTCGTCTCC 
ACAAGCACAG ACTCACCCAC GTCACCGATC GCAGAGGCGC CCCCGCTGCC TCCCAGAAAC 
GCCACGAAAG GTCCACCTGG CCCACCTTCA ACACTCCCTC TAAGCACCCA GACCTCTAGT 
GGCAGCTCCA CCCTGTCCAA GAAGCGGTCT CCTCCCCCAC CACCCGGACA CAAGAGAACC 
CTGTCTGACC CTCCCAGCCC ACTACCTCAC GGGCCCCCAA ACAAAGGCGC AGTTCCTTGG 
GGTAACGACG TGGGTCCCTC ATCGTCCAGT AAGACCACGA ACAAGTTCGA GGGCCTGTCC 
CAGCAGTCGA GCACCGGTTC TGCAAAGACT GCACTTGGCC CAAGAGTTCT TCCTAAACTA 
CCTCAGAAAG TGGCACTAAG GAAAACAGAG ACCAGCCATC ATCTCTCCCT CGACAAAGCC 
AACGTCCCAC CTGAGATCTT CCAGAAGTCG TCCCAGTTGA CAGAGTTACC GCAGAAGCCG 
CCACCCGGGG ACCTGCCCCC GAAGCCCACG GAACTGGCTC CCAAACCCCC CATTGGAGAC 
TTACCACCTA AGCCAGGCGA GCTGCCCCCG AAGCCACAGC TGGGCGACCT GCCCCCCAAG 
CCCCAGCTCG CAGACTTGCC CCCCAAGCCC CAGGTGAAAG ACCTGCCTCC CAAGCCACAA 
CTGGGGGAGC TGCTGGCAAA ACCCCAGACG GGAGACGCCT CGCCCAAGGC CCAGCCACCC 
CTGGAGCTCA CCCCCAAGTC ACACCCGGCG GACCTGTCCC CGAACGTCCC CAAGCAGGCG 
TCTGAGGACA CCAACGACCT CACGCCCACC CTGCCAGAGA CACCCGTGCC TCTGCCCAGG 
AAGATCAACA CGGGGAAGAG CAAGGTGAGG CGAGTGAAGA CCATCTACGA CTGCCAGGCG 
GACAACGATG ACGAGCTGAC TTTCATGGAG GGCGAGGTGA TCGTGGTCAC CGGGGAGGAG 
GACCAGGAGT GGTGGATTGG GCACATCGAG GGGCAGCCCG AGAGGAAGGG CGTCTTCCCA 
GTGTCCTTTG TCCACATCCT GTCGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASAP1 ( XM_015474408.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329894.1 Bos taurus ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (ASAP1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa92491
Clone ID Related Accession (Same CDS sequence)	XM_015474408.1
Accession Version	XM_015474408.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3381bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104383.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATCTT CAGCCTCCAG GCTCTCCAGT TTTTCATCAA GAGATTCGCT ATGGAATCGG 
ATGCCGGACC AGATCTCCGT CTCCGAGTTC ATCGCCGAGA CCACCGAGGA CTACAACTCG 
CCCACCACGT CCAGCTTCAC TACGCGGCTG CACAACTGCA GGAACACCGT CACGCTGCTG 
GAGGAGGCTC TAGACCAAGA TAGAACAGCC TTACAGAAAG TTAAGAAGTC TGTAAAAGCA 
ATATACAATT CCGGTCAAGA CCATGTACAA AATGAAGAAA ACTATGCACA AGTTCTTGAT 
AAGTTTGGGA GTAATTTTTT AAGTCGAGAC AACCCAGATC TTGGCACCGC TTTTGTCAAG 
TTTTCTACGC TTACAAAGGA ACTGTCCACA CTGCTGAAAA ATCTGCTCCA GGGCCTGAGC 
CACAATGTGA TCTTCACCTT GGATTCCTTG TTGAAAGGAG ACCTGAAGGG AGTCAAAGGC 
GATCTCAAGA AACCATTTGA CAAAGCTTGG AAAGATTATG AGACGAAGTT TACCAAAATT 
GAGAAGGAGA AGAGGGAGCA CGCCAAGCAG CACGGGATGA TCCGCACGGA GATCACCGGC 
GCCGAGATCG CGGAGGAAAT GGAGAAGGAG CGGCGCCTCT TCCAGCTCCA GATGTGCGAG 
TATCTCATTA AAGTTAATGA AATCAAGACC AAAAAGGGTG TGGATCTGCT GCAGAACCTG 
ATAAAGTATT ATCACGCACA GTGCAATTTC TTTCAAGATG GTTTGAAAAC AGCTGATAAA 
TTGAAACAGT ACATTGAAAA GCTGGCTGCT GATTTGTATA ATATCAAACA GACCCAGGAC 
GAAGAAAAGA AACAGCTGAC CGCACTCCGA GACCTAATAA AGTCCTCGCT CCAACTCGAT 
CAGAAAGAAG ATTCCCAGAG CCGGCAGGGA GGCTACAGCA TGCACCAGCT GCAGGGCAAC 
AAGGAATACG GCAGCGAGAA GAAGGGCTAC CTGCTGAAGA AGAGTGACGG GATCCGGAAA 
GTGTGGCAGA GAAGGAAGTG CTCCGTCAAG AACGGGATCC TGACCATCTC CCACGCCACG 
TCCAACAGAC AGCCAGCCAA GCTGAACCTT CTCACTTGCC AGGTGAAGCC GAATGCCGAG 
GACAAGAAGT CTTTCGACCT GATATCACAT AACAGGACGT ATCACTTTCA GGCCGAAGAT 
GAGCAGGATT ATGTAGCGTG GATCTCGGTG CTGACGAACA GCAAAGAGGA GGCCCTCACC 
ATGGCCTTCC GGGGGGAACA GAGTGCTGGG GAGAGCAGCC TGGAGGAGCT GACGAAGGCC 
ATCATCGAGG ACGTGCAGCG GCTCCCGGGC AACGACGTCT GCTGCGACTG CGGCTCGGCA 
GAACCCACCT GGCTGTCCAC CAACTTGGGT ATCTTGACCT GTATAGAATG TTCCGGCATC 
CATAGAGAAA TGGGGGTTCA TATTTCTCGC ATCCAGTCTT TGGAACTAGA CAAATTAGGA 
ACTTCTGAAC TCTTGCTGGC CAAGAATGTA GGAAACAATA GTTTTAATGA TATTATGGAA 
GCAAATTTAC CCAGTCCCTC ACCAAAACCC ACCCCTTCAA GTGATATGAC CGTACGGAAG 
GAATATATCA CTGCAAAGTA TGTAGATCAT AGGTTTTCAC GGAAGACCTG TTCATCCTCA 
TCAGCTAAAC TGAACGAATT GCTTGAGGCC ATCAAATCCA GGGATTTACT TGCACTAATT 
CAAGTCTATG CAGAGGGGGT GGAGCTAATG GAACCGCTGC TGGAACCCGG ACAGGAGCTT 
GGGGAGACAG CCCTTCATCT TGCAGTCCGA ACCGCAGACC AGACATCTCT CCATTTGGTG 
GACTTCCTTG TACAAAACTG TGGGAACCTA GATAAGCAGA CGGCCCTGGG GAACACGGCC 
CTGCACTACT GTAGTATGTA CAGTAAACCA GAGTGTTTGA AGCTGCTGCT CAGGAGCAAG 
CCCACTGTGG ACGTCGTTAA TCAGGCTGGA GAGACCGCCC TGGACATAGC AAAGAGACTG 
AAAGCCACTC AGTGTGAAGA CCTGCTTTCC CAAGCTAAAT CTGGAAAGTT CAATCCACAC 
GTCCACGTGG AATATGAGTG GAATCTTCGA CAGGAGGAGA TGGATGAGAG CGATGACGAC 
CTGGATGACA AACCGAGCCC CATCAAGAAG GAGCGCTCCC CCCGACCGCA GAGCTTCTGC 
CACTCCTCCA GCATCTCCCC CCAGGACAAG CTCTCACTGC CGGGCTTCAG CACGCCAAGG 
GACAAGCAAC GACTCTCCTA CGGCGCCTTC ACCAACCAGA TCTTCGTCTC CACAAGCACA 
GACTCACCCA CGTCACCGAT CGCAGAGGCG CCCCCGCTGC CTCCCAGAAA CGCCACGAAA 
GGTCCACCTG GCCCACCTTC AACACTCCCT CTAAGCACCC AGACCTCTAG TGGCAGCTCC 
ACCCTGTCCA AGAAGCGGCC TCCTCCCCCA CCACCCGGAC ACAAGAGAAC CCTGTCTGAC 
CCTCCCAGCC CACTACCTCA CGGGCCCCCA AACAAAGGCG CAGTTCCTTG GGGTAACGAC 
GTGGGTCCCT CATCGTCCAG TAAGACCACG AACAAGTTCG AGGGCCTGTC CCAGCAGTCG 
AGCACCGGTT CTGCAAAGAC TGCACTTGGC CCAAGAGTTC TTCCTAAACT ACCTCAGAAA 
GTGGCACTAA GGAAAACAGA GACCAGCCAT CATCTCTCCC TCGACAAAGC CAACGTCCCA 
CCTGAGATCT TCCAGAAGTC GTCCCAGTTG ACAGAGTTAC CGCAGAAGCC GCCACCCGGG 
GACCTGCCCC CGAAGCCCAC GGAACTGGCT CCCAAACCCC CCATTGGAGA CTTACCACCT 
AAGCCAGGCG AGCTGCCCCC GAAGCCACAG CTGGGCGACC TGCCCCCCAA GCCCCAGCTC 
GCAGACTTGC CCCCCAAGCC CCAGGTGAAA GACCTGCCTC CCAAGCCACA ACTGGGGGAG 
CTGCTGGCAA AACCCCAGAC GGGAGACGCC TCGCCCAAGG CCCAGCCACC CCTGGAGCTC 
ACCCCCAAGT CACACCCGGC GGACCTGTCC CCGAACGTCC CCAAGCAGGC GTCTGAGGAC 
ACCAACGACC TCACGCCCAC CCTGCCAGAG ACACCTGTGC CGCTGCCCAG GAAGATCAAC 
ACGGGGAAGA GCAAGGTGAG GCGAGTGAAG ACCATCTACG ACTGCCAGGC GGACAACGAT 
GACGAGCTGA CTTTCATGGA GGGCGAGGTG ATCGTGGTCA CCGGGGAGGA GGACCAGGAG 
TGGTGGATTG GGCACATCGA GGGGCAGCCC GAGAGGAAGG GCGTCTTCCC AGTGTCCTTT 
GTCCACATCC TGTCGGACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329894.1
CDS	133..3513
Translation	MRSSASRLSSFSSRDSLWNRMPDQISVSEFIAETTEDYNSPTTSSFTTRLHNCRNTVTLLEEALDQDRTALQKVKKSVKAIYNSGQDHVQNEENYAQVLDKFGSNFLSRDNPDLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGVKGDLKKPFDKAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDQKEDSQSRQGGYSMHQLQGNKEYGSEKKGYLLKKSDGIRKVWQRRKCSVKNGILTISHATSNRQPAKLNLLTCQVKPNAEDKKSFDLISHNRTYHFQAEDEQDYVAWISVLTNSKEEALTMAFRGEQSAGESSLEELTKAIIEDVQRLPGNDVCCDCGSAEPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDKLGTSELLLAKNVGNNSFNDIMEANLPSPSPKPTPSSDMTVRKEYITAKYVDHRFSRKTCSSSSAKLNELLEAIKSRDLLALIQVYAEGVELMEPLLEPGQELGETALHLAVRTADQTSLHLVDFLVQNCGNLDKQTALGNTALHYCSMYSKPECLKLLLRSKPTVDVVNQAGETALDIAKRLKATQCEDLLSQAKSGKFNPHVHVEYEWNLRQEEMDESDDDLDDKPSPIKKERSPRPQSFCHSSSISPQDKLSLPGFSTPRDKQRLSYGAFTNQIFVSTSTDSPTSPIAEAPPLPPRNATKGPPGPPSTLPLSTQTSSGSSTLSKKRPPPPPPGHKRTLSDPPSPLPHGPPNKGAVPWGNDVGPSSSSKTTNKFEGLSQQSSTGSAKTALGPRVLPKLPQKVALRKTETSHHLSLDKANVPPEIFQKSSQLTELPQKPPPGDLPPKPTELAPKPPIGDLPPKPGELPPKPQLGDLPPKPQLADLPPKPQVKDLPPKPQLGELLAKPQTGDASPKAQPPLELTPKSHPADLSPNVPKQASEDTNDLTPTLPETPVPLPRKINTGKSKVRRVKTIYDCQADNDDELTFMEGEVIVVTGEEDQEWWIGHIEGQPERKGVFPVSFVHILSD

Target ORF information:

RefSeq Version	XM_015474408.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (ASAP1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474408.1

ORF Insert Sequence:

ATGAGATCTT CAGCCTCCAG GCTCTCCAGT TTTTCATCAA GAGATTCGCT ATGGAATCGG 
ATGCCGGACC AGATCTCCGT CTCCGAGTTC ATCGCCGAGA CCACCGAGGA CTACAACTCG 
CCCACCACGT CCAGCTTCAC TACGCGGCTG CACAACTGCA GGAACACCGT CACGCTGCTG 
GAGGAGGCTC TAGACCAAGA TAGAACAGCC TTACAGAAAG TTAAGAAGTC TGTAAAAGCA 
ATATACAATT CCGGTCAAGA CCATGTACAA AATGAAGAAA ACTATGCACA AGTTCTTGAT 
AAGTTTGGGA GTAATTTTTT AAGTCGAGAC AACCCAGATC TTGGCACCGC TTTTGTCAAG 
TTTTCTACGC TTACAAAGGA ACTGTCCACA CTGCTGAAAA ATCTGCTCCA GGGCCTGAGC 
CACAATGTGA TCTTCACCTT GGATTCCTTG TTGAAAGGAG ACCTGAAGGG AGTCAAAGGC 
GATCTCAAGA AACCATTTGA CAAAGCTTGG AAAGATTATG AGACGAAGTT TACCAAAATT 
GAGAAGGAGA AGAGGGAGCA CGCCAAGCAG CACGGGATGA TCCGCACGGA GATCACCGGC 
GCCGAGATCG CGGAGGAAAT GGAGAAGGAG CGGCGCCTCT TCCAGCTCCA GATGTGCGAG 
TATCTCATTA AAGTTAATGA AATCAAGACC AAAAAGGGTG TGGATCTGCT GCAGAACCTG 
ATAAAGTATT ATCACGCACA GTGCAATTTC TTTCAAGATG GTTTGAAAAC AGCTGATAAA 
TTGAAACAGT ACATTGAAAA GCTGGCTGCT GATTTGTATA ATATCAAACA GACCCAGGAC 
GAAGAAAAGA AACAGCTGAC CGCACTCCGA GACCTAATAA AGTCCTCGCT CCAACTCGAT 
CAGAAAGAAG ATTCCCAGAG CCGGCAGGGA GGCTACAGCA TGCACCAGCT GCAGGGCAAC 
AAGGAATACG GCAGCGAGAA GAAGGGCTAC CTGCTGAAGA AGAGTGACGG GATCCGGAAA 
GTGTGGCAGA GAAGGAAGTG CTCCGTCAAG AACGGGATCC TGACCATCTC CCACGCCACG 
TCCAACAGAC AGCCAGCCAA GCTGAACCTT CTCACTTGCC AGGTGAAGCC GAATGCCGAG 
GACAAGAAGT CTTTCGACCT GATATCACAT AACAGGACGT ATCACTTTCA GGCCGAAGAT 
GAGCAGGATT ATGTAGCGTG GATCTCGGTG CTGACGAACA GCAAAGAGGA GGCCCTCACC 
ATGGCCTTCC GGGGGGAACA GAGTGCTGGG GAGAGCAGCC TGGAGGAGCT GACGAAGGCC 
ATCATCGAGG ACGTGCAGCG GCTCCCGGGC AACGACGTCT GCTGCGACTG CGGCTCGGCA 
GAACCCACCT GGCTGTCCAC CAACTTGGGT ATCTTGACCT GTATAGAATG TTCCGGCATC 
CATAGAGAAA TGGGGGTTCA TATTTCTCGC ATCCAGTCTT TGGAACTAGA CAAATTAGGA 
ACTTCTGAAC TCTTGCTGGC CAAGAATGTA GGAAACAATA GTTTTAATGA TATTATGGAA 
GCAAATTTAC CCAGTCCCTC ACCAAAACCC ACCCCTTCAA GTGATATGAC CGTACGGAAG 
GAATATATCA CTGCAAAGTA TGTAGATCAT AGGTTTTCAC GGAAGACCTG TTCATCCTCA 
TCAGCTAAAC TGAACGAATT GCTTGAGGCC ATCAAATCCA GGGATTTACT TGCACTAATT 
CAAGTCTATG CAGAGGGGGT GGAGCTAATG GAACCGCTGC TGGAACCCGG ACAGGAGCTT 
GGGGAGACAG CCCTTCATCT TGCAGTCCGA ACCGCAGACC AGACATCTCT CCATTTGGTG 
GACTTCCTTG TACAAAACTG TGGGAACCTA GATAAGCAGA CGGCCCTGGG GAACACGGCC 
CTGCACTACT GTAGTATGTA CAGTAAACCA GAGTGTTTGA AGCTGCTGCT CAGGAGCAAG 
CCCACTGTGG ACGTCGTTAA TCAGGCTGGA GAGACCGCCC TGGACATAGC AAAGAGACTG 
AAAGCCACTC AGTGTGAAGA CCTGCTTTCC CAAGCTAAAT CTGGAAAGTT CAATCCACAC 
GTCCACGTGG AATATGAGTG GAATCTTCGA CAGGAGGAGA TGGATGAGAG CGATGACGAC 
CTGGATGACA AACCGAGCCC CATCAAGAAG GAGCGCTCCC CCCGACCGCA GAGCTTCTGC 
CACTCCTCCA GCATCTCCCC CCAGGACAAG CTCTCACTGC CGGGCTTCAG CACGCCAAGG 
GACAAGCAAC GACTCTCCTA CGGCGCCTTC ACCAACCAGA TCTTCGTCTC CACAAGCACA 
GACTCACCCA CGTCACCGAT CGCAGAGGCG CCCCCGCTGC CTCCCAGAAA CGCCACGAAA 
GGTCCACCTG GCCCACCTTC AACACTCCCT CTAAGCACCC AGACCTCTAG TGGCAGCTCC 
ACCCTGTCCA AGAAGCGGCC TCCTCCCCCA CCACCCGGAC ACAAGAGAAC CCTGTCTGAC 
CCTCCCAGCC CACTACCTCA CGGGCCCCCA AACAAAGGCG CAGTTCCTTG GGGTAACGAC 
GTGGGTCCCT CATCGTCCAG TAAGACCACG AACAAGTTCG AGGGCCTGTC CCAGCAGTCG 
AGCACCGGTT CTGCAAAGAC TGCACTTGGC CCAAGAGTTC TTCCTAAACT ACCTCAGAAA 
GTGGCACTAA GGAAAACAGA GACCAGCCAT CATCTCTCCC TCGACAAAGC CAACGTCCCA 
CCTGAGATCT TCCAGAAGTC GTCCCAGTTG ACAGAGTTAC CGCAGAAGCC GCCACCCGGG 
GACCTGCCCC CGAAGCCCAC GGAACTGGCT CCCAAACCCC CCATTGGAGA CTTACCACCT 
AAGCCAGGCG AGCTGCCCCC GAAGCCACAG CTGGGCGACC TGCCCCCCAA GCCCCAGCTC 
GCAGACTTGC CCCCCAAGCC CCAGGTGAAA GACCTGCCTC CCAAGCCACA ACTGGGGGAG 
CTGCTGGCAA AACCCCAGAC GGGAGACGCC TCGCCCAAGG CCCAGCCACC CCTGGAGCTC 
ACCCCCAAGT CACACCCGGC GGACCTGTCC CCGAACGTCC CCAAGCAGGC GTCTGAGGAC 
ACCAACGACC TCACGCCCAC CCTGCCAGAG ACACCTGTGC CGCTGCCCAG GAAGATCAAC 
ACGGGGAAGA GCAAGGTGAG GCGAGTGAAG ACCATCTACG ACTGCCAGGC GGACAACGAT 
GACGAGCTGA CTTTCATGGA GGGCGAGGTG ATCGTGGTCA CCGGGGAGGA GGACCAGGAG 
TGGTGGATTG GGCACATCGA GGGGCAGCCC GAGAGGAAGG GCGTCTTCCC AGTGTCCTTT 
GTCCACATCC TGTCGGACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASAP1 ( XM_015474409.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329895.1 Bos taurus ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (ASAP1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa92492
Clone ID Related Accession (Same CDS sequence)	XM_015474409.1
Accession Version	XM_015474409.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3303bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104383.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGTCC AGTCTTATAA GGAAACCACG GGCAGCTCTA AGCAAGAAGG GAGAAGCTCT 
CCTGCATCCG ATCCCCTGTG TTTCTGGGGT TTTATCCAGG CTCTAGACCA AGATAGAACA 
GCCTTACAGA AAGTTAAGAA GTCTGTAAAA GCAATATACA ATTCCGGTCA AGACCATGTA 
CAAAATGAAG AAAACTATGC ACAAGTTCTT GATAAGTTTG GGAGTAATTT TTTAAGTCGA 
GACAACCCAG ATCTTGGCAC CGCTTTTGTC AAGTTTTCTA CGCTTACAAA GGAACTGTCC 
ACACTGCTGA AAAATCTGCT CCAGGGCCTG AGCCACAATG TGATCTTCAC CTTGGATTCC 
TTGTTGAAAG GAGACCTGAA GGGAGTCAAA GGCGATCTCA AGAAACCATT TGACAAAGCT 
TGGAAAGATT ATGAGACGAA GTTTACCAAA ATTGAGAAGG AGAAGAGGGA GCACGCCAAG 
CAGCACGGGA TGATCCGCAC GGAGATCACC GGCGCCGAGA TCGCGGAGGA AATGGAGAAG 
GAGCGGCGCC TCTTCCAGCT CCAGATGTGC GAGTATCTCA TTAAAGTTAA TGAAATCAAG 
ACCAAAAAGG GTGTGGATCT GCTGCAGAAC CTGATAAAGT ATTATCACGC ACAGTGCAAT 
TTCTTTCAAG ATGGTTTGAA AACAGCTGAT AAATTGAAAC AGTACATTGA AAAGCTGGCT 
GCTGATTTGT ATAATATCAA ACAGACCCAG GACGAAGAAA AGAAACAGCT GACCGCACTC 
CGAGACCTAA TAAAGTCCTC GCTCCAACTC GATCAGAAAG AATCTAGGAG AGATTCCCAG 
AGCCGGCAGG GAGGCTACAG CATGCACCAG CTGCAGGGCA ACAAGGAATA CGGCAGCGAG 
AAGAAGGGCT ACCTGCTGAA GAAGAGTGAC GGGATCCGGA AAGTGTGGCA GAGAAGGAAG 
TGCTCCGTCA AGAACGGGAT CCTGACCATC TCCCACGCCA CGTCCAACAG ACAGCCAGCC 
AAGCTGAACC TTCTCACTTG CCAGGTGAAG CCGAATGCCG AGGACAAGAA GTCTTTCGAC 
CTGATATCAC ATAACAGGAC GTATCACTTT CAGGCCGAAG ATGAGCAGGA TTATGTAGCG 
TGGATCTCGG TGCTGACGAA CAGCAAAGAG GAGGCCCTCA CCATGGCCTT CCGGGGGGAA 
CAGAGTGCTG GGGAGAGCAG CCTGGAGGAG CTGACGAAGG CCATCATCGA GGACGTGCAG 
CGGCTCCCGG GCAACGACGT CTGCTGCGAC TGCGGCTCGG CAGAACCCAC CTGGCTGTCC 
ACCAACTTGG GTATCTTGAC CTGTATAGAA TGTTCCGGCA TCCATAGAGA AATGGGGGTT 
CATATTTCTC GCATCCAGTC TTTGGAACTA GACAAATTAG GAACTTCTGA ACTCTTGCTG 
GCCAAGAATG TAGGAAACAA TAGTTTTAAT GATATTATGG AAGCAAATTT ACCCAGTCCC 
TCACCAAAAC CCACCCCTTC AAGTGATATG ACCGTACGGA AGGAATATAT CACTGCAAAG 
TATGTAGATC ATAGGTTTTC ACGGAAGACC TGTTCATCCT CATCAGCTAA ACTGAACGAA 
TTGCTTGAGG CCATCAAATC CAGGGATTTA CTTGCACTAA TTCAAGTCTA TGCAGAGGGG 
GTGGAGCTAA TGGAACCGCT GCTGGAACCC GGACAGGAGC TTGGGGAGAC AGCCCTTCAT 
CTTGCAGTCC GAACCGCAGA CCAGACATCT CTCCATTTGG TGGACTTCCT TGTACAAAAC 
TGTGGGAACC TAGATAAGCA GACGGCCCTG GGGAACACGG CCCTGCACTA CTGTAGTATG 
TACAGTAAAC CAGAGTGTTT GAAGCTGCTG CTCAGGAGCA AGCCCACTGT GGACGTCGTT 
AATCAGGCTG GAGAGACCGC CCTGGACATA GCAAAGAGAC TGAAAGCCAC TCAGTGTGAA 
GACCTGCTTT CCCAAGCTAA ATCTGGAAAG TTCAATCCAC ACGTCCACGT GGAATATGAG 
TGGAATCTTC GACAGGAGGA GATGGATGAG AGCGATGACG ACCTGGATGA CAAACCGAGC 
CCCATCAAGA AGGAGCGCTC CCCCCGACCG CAGAGCTTCT GCCACTCCTC CAGCATCTCC 
CCCCAGGACA AGCTCTCACT GCCGGGCTTC AGCACGCCAA GGGACAAGCA ACGACTCTCC 
TACGGCGCCT TCACCAACCA GATCTTCGTC TCCACAAGCA CAGACTCACC CACGTCACCG 
ATCGCAGAGG CGCCCCCGCT GCCTCCCAGA AACGCCACGA AAGGTCCACC TGGCCCACCT 
TCAACACTCC CTCTAAGCAC CCAGACCTCT AGTGGCAGCT CCACCCTGTC CAAGAAGCGG 
CCTCCTCCCC CACCACCCGG ACACAAGAGA ACCCTGTCTG ACCCTCCCAG CCCACTACCT 
CACGGGCCCC CAAACAAAGG CGCAGTTCCT TGGGGTAACG ACGTGGGTCC CTCATCGTCC 
AGTAAGACCA CGAACAAGTT CGAGGGCCTG TCCCAGCAGT CGAGCACCGG TTCTGCAAAG 
ACTGCACTTG GCCCAAGAGT TCTTCCTAAA CTACCTCAGA AAGTGGCACT AAGGAAAACA 
GAGACCAGCC ATCATCTCTC CCTCGACAAA GCCAACGTCC CACCTGAGAT CTTCCAGAAG 
TCGTCCCAGT TGACAGAGTT ACCGCAGAAG CCGCCACCCG GGGACCTGCC CCCGAAGCCC 
ACGGAACTGG CTCCCAAACC CCCCATTGGA GACTTACCAC CTAAGCCAGG CGAGCTGCCC 
CCGAAGCCAC AGCTGGGCGA CCTGCCCCCC AAGCCCCAGC TCGCAGACTT GCCCCCCAAG 
CCCCAGGTGA AAGACCTGCC TCCCAAGCCA CAACTGGGGG AGCTGCTGGC AAAACCCCAG 
ACGGGAGACG CCTCGCCCAA GGCCCAGCCA CCCCTGGAGC TCACCCCCAA GTCACACCCG 
GCGGACCTGT CCCCGAACGT CCCCAAGCAG GCGTCTGAGG ACACCAACGA CCTCACGCCC 
ACCCTGCCAG AGACACCTGT GCCGCTGCCC AGGAAGATCA ACACGGGGAA GAGCAAGGTG 
AGGCGAGTGA AGACCATCTA CGACTGCCAG GCGGACAACG ATGACGAGCT GACTTTCATG 
GAGGGCGAGG TGATCGTGGT CACCGGGGAG GAGGACCAGG AGTGGTGGAT TGGGCACATC 
GAGGGGCAGC CCGAGAGGAA GGGCGTCTTC CCAGTGTCCT TTGTCCACAT CCTGTCGGAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329895.1
CDS	1614..4916
Translation	MGVQSYKETTGSSKQEGRSSPASDPLCFWGFIQALDQDRTALQKVKKSVKAIYNSGQDHVQNEENYAQVLDKFGSNFLSRDNPDLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGVKGDLKKPFDKAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDQKESRRDSQSRQGGYSMHQLQGNKEYGSEKKGYLLKKSDGIRKVWQRRKCSVKNGILTISHATSNRQPAKLNLLTCQVKPNAEDKKSFDLISHNRTYHFQAEDEQDYVAWISVLTNSKEEALTMAFRGEQSAGESSLEELTKAIIEDVQRLPGNDVCCDCGSAEPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDKLGTSELLLAKNVGNNSFNDIMEANLPSPSPKPTPSSDMTVRKEYITAKYVDHRFSRKTCSSSSAKLNELLEAIKSRDLLALIQVYAEGVELMEPLLEPGQELGETALHLAVRTADQTSLHLVDFLVQNCGNLDKQTALGNTALHYCSMYSKPECLKLLLRSKPTVDVVNQAGETALDIAKRLKATQCEDLLSQAKSGKFNPHVHVEYEWNLRQEEMDESDDDLDDKPSPIKKERSPRPQSFCHSSSISPQDKLSLPGFSTPRDKQRLSYGAFTNQIFVSTSTDSPTSPIAEAPPLPPRNATKGPPGPPSTLPLSTQTSSGSSTLSKKRPPPPPPGHKRTLSDPPSPLPHGPPNKGAVPWGNDVGPSSSSKTTNKFEGLSQQSSTGSAKTALGPRVLPKLPQKVALRKTETSHHLSLDKANVPPEIFQKSSQLTELPQKPPPGDLPPKPTELAPKPPIGDLPPKPGELPPKPQLGDLPPKPQLADLPPKPQVKDLPPKPQLGELLAKPQTGDASPKAQPPLELTPKSHPADLSPNVPKQASEDTNDLTPTLPETPVPLPRKINTGKSKVRRVKTIYDCQADNDDELTFMEGEVIVVTGEEDQEWWIGHIEGQPERKGVFPVSFVHILSD

Target ORF information:

RefSeq Version	XM_015474409.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (ASAP1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474409.1

ORF Insert Sequence:

ATGGGGGTCC AGTCTTATAA GGAAACCACG GGCAGCTCTA AGCAAGAAGG GAGAAGCTCT 
CCTGCATCCG ATCCCCTGTG TTTCTGGGGT TTTATCCAGG CTCTAGACCA AGATAGAACA 
GCCTTACAGA AAGTTAAGAA GTCTGTAAAA GCAATATACA ATTCCGGTCA AGACCATGTA 
CAAAATGAAG AAAACTATGC ACAAGTTCTT GATAAGTTTG GGAGTAATTT TTTAAGTCGA 
GACAACCCAG ATCTTGGCAC CGCTTTTGTC AAGTTTTCTA CGCTTACAAA GGAACTGTCC 
ACACTGCTGA AAAATCTGCT CCAGGGCCTG AGCCACAATG TGATCTTCAC CTTGGATTCC 
TTGTTGAAAG GAGACCTGAA GGGAGTCAAA GGCGATCTCA AGAAACCATT TGACAAAGCT 
TGGAAAGATT ATGAGACGAA GTTTACCAAA ATTGAGAAGG AGAAGAGGGA GCACGCCAAG 
CAGCACGGGA TGATCCGCAC GGAGATCACC GGCGCCGAGA TCGCGGAGGA AATGGAGAAG 
GAGCGGCGCC TCTTCCAGCT CCAGATGTGC GAGTATCTCA TTAAAGTTAA TGAAATCAAG 
ACCAAAAAGG GTGTGGATCT GCTGCAGAAC CTGATAAAGT ATTATCACGC ACAGTGCAAT 
TTCTTTCAAG ATGGTTTGAA AACAGCTGAT AAATTGAAAC AGTACATTGA AAAGCTGGCT 
GCTGATTTGT ATAATATCAA ACAGACCCAG GACGAAGAAA AGAAACAGCT GACCGCACTC 
CGAGACCTAA TAAAGTCCTC GCTCCAACTC GATCAGAAAG AATCTAGGAG AGATTCCCAG 
AGCCGGCAGG GAGGCTACAG CATGCACCAG CTGCAGGGCA ACAAGGAATA CGGCAGCGAG 
AAGAAGGGCT ACCTGCTGAA GAAGAGTGAC GGGATCCGGA AAGTGTGGCA GAGAAGGAAG 
TGCTCCGTCA AGAACGGGAT CCTGACCATC TCCCACGCCA CGTCCAACAG ACAGCCAGCC 
AAGCTGAACC TTCTCACTTG CCAGGTGAAG CCGAATGCCG AGGACAAGAA GTCTTTCGAC 
CTGATATCAC ATAACAGGAC GTATCACTTT CAGGCCGAAG ATGAGCAGGA TTATGTAGCG 
TGGATCTCGG TGCTGACGAA CAGCAAAGAG GAGGCCCTCA CCATGGCCTT CCGGGGGGAA 
CAGAGTGCTG GGGAGAGCAG CCTGGAGGAG CTGACGAAGG CCATCATCGA GGACGTGCAG 
CGGCTCCCGG GCAACGACGT CTGCTGCGAC TGCGGCTCGG CAGAACCCAC CTGGCTGTCC 
ACCAACTTGG GTATCTTGAC CTGTATAGAA TGTTCCGGCA TCCATAGAGA AATGGGGGTT 
CATATTTCTC GCATCCAGTC TTTGGAACTA GACAAATTAG GAACTTCTGA ACTCTTGCTG 
GCCAAGAATG TAGGAAACAA TAGTTTTAAT GATATTATGG AAGCAAATTT ACCCAGTCCC 
TCACCAAAAC CCACCCCTTC AAGTGATATG ACCGTACGGA AGGAATATAT CACTGCAAAG 
TATGTAGATC ATAGGTTTTC ACGGAAGACC TGTTCATCCT CATCAGCTAA ACTGAACGAA 
TTGCTTGAGG CCATCAAATC CAGGGATTTA CTTGCACTAA TTCAAGTCTA TGCAGAGGGG 
GTGGAGCTAA TGGAACCGCT GCTGGAACCC GGACAGGAGC TTGGGGAGAC AGCCCTTCAT 
CTTGCAGTCC GAACCGCAGA CCAGACATCT CTCCATTTGG TGGACTTCCT TGTACAAAAC 
TGTGGGAACC TAGATAAGCA GACGGCCCTG GGGAACACGG CCCTGCACTA CTGTAGTATG 
TACAGTAAAC CAGAGTGTTT GAAGCTGCTG CTCAGGAGCA AGCCCACTGT GGACGTCGTT 
AATCAGGCTG GAGAGACCGC CCTGGACATA GCAAAGAGAC TGAAAGCCAC TCAGTGTGAA 
GACCTGCTTT CCCAAGCTAA ATCTGGAAAG TTCAATCCAC ACGTCCACGT GGAATATGAG 
TGGAATCTTC GACAGGAGGA GATGGATGAG AGCGATGACG ACCTGGATGA CAAACCGAGC 
CCCATCAAGA AGGAGCGCTC CCCCCGACCG CAGAGCTTCT GCCACTCCTC CAGCATCTCC 
CCCCAGGACA AGCTCTCACT GCCGGGCTTC AGCACGCCAA GGGACAAGCA ACGACTCTCC 
TACGGCGCCT TCACCAACCA GATCTTCGTC TCCACAAGCA CAGACTCACC CACGTCACCG 
ATCGCAGAGG CGCCCCCGCT GCCTCCCAGA AACGCCACGA AAGGTCCACC TGGCCCACCT 
TCAACACTCC CTCTAAGCAC CCAGACCTCT AGTGGCAGCT CCACCCTGTC CAAGAAGCGG 
CCTCCTCCCC CACCACCCGG ACACAAGAGA ACCCTGTCTG ACCCTCCCAG CCCACTACCT 
CACGGGCCCC CAAACAAAGG CGCAGTTCCT TGGGGTAACG ACGTGGGTCC CTCATCGTCC 
AGTAAGACCA CGAACAAGTT CGAGGGCCTG TCCCAGCAGT CGAGCACCGG TTCTGCAAAG 
ACTGCACTTG GCCCAAGAGT TCTTCCTAAA CTACCTCAGA AAGTGGCACT AAGGAAAACA 
GAGACCAGCC ATCATCTCTC CCTCGACAAA GCCAACGTCC CACCTGAGAT CTTCCAGAAG 
TCGTCCCAGT TGACAGAGTT ACCGCAGAAG CCGCCACCCG GGGACCTGCC CCCGAAGCCC 
ACGGAACTGG CTCCCAAACC CCCCATTGGA GACTTACCAC CTAAGCCAGG CGAGCTGCCC 
CCGAAGCCAC AGCTGGGCGA CCTGCCCCCC AAGCCCCAGC TCGCAGACTT GCCCCCCAAG 
CCCCAGGTGA AAGACCTGCC TCCCAAGCCA CAACTGGGGG AGCTGCTGGC AAAACCCCAG 
ACGGGAGACG CCTCGCCCAA GGCCCAGCCA CCCCTGGAGC TCACCCCCAA GTCACACCCG 
GCGGACCTGT CCCCGAACGT CCCCAAGCAG GCGTCTGAGG ACACCAACGA CCTCACGCCC 
ACCCTGCCAG AGACACCTGT GCCGCTGCCC AGGAAGATCA ACACGGGGAA GAGCAAGGTG 
AGGCGAGTGA AGACCATCTA CGACTGCCAG GCGGACAACG ATGACGAGCT GACTTTCATG 
GAGGGCGAGG TGATCGTGGT CACCGGGGAG GAGGACCAGG AGTGGTGGAT TGGGCACATC 
GAGGGGCAGC CCGAGAGGAA GGGCGTCTTC CCAGTGTCCT TTGTCCACAT CCTGTCGGAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASAP1 ( XM_015474410.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329896.1 Bos taurus ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (ASAP1), transcript variant X3, mRNA.

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CloneID	OBa92493
Clone ID Related Accession (Same CDS sequence)	XM_015474410.1
Accession Version	XM_015474410.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3219bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104383.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATCTT CAGCCTCCAG GCTCTCCAGT TTTTCATCAA GAGATTCGCT ATGGAATCGG 
ATGCCGGACC AGATCTCCGT CTCCGAGTTC ATCGCCGAGA CCACCGAGGA CTACAACTCG 
CCCACCACGT CCAGCTTCAC TACGCGGCTG CACAACTGCA GGAACACCGT CACGCTGCTG 
GAGGAGGCTC TAGACCAAGA TAGAACAGCC TTACAGAAAG TTAAGAAGTC TGTAAAAGCA 
ATATACAATT CCGGTCAAGA CCATGTACAA AATGAAGAAA ACTATGCACA AGTTCTTGAT 
AAGTTTGGGA GTAATTTTTT AAGTCGAGAC AACCCAGATC TTGGCACCGC TTTTGTCAAG 
TTTTCTACGC TTACAAAGGA ACTGTCCACA CTGCTGAAAA ATCTGCTCCA GGGCCTGAGC 
CACAATGTGA TCTTCACCTT GGATTCCTTG TTGAAAGGAG ACCTGAAGGG AGTCAAAGGC 
GATCTCAAGA AACCATTTGA CAAAGCTTGG AAAGATTATG AGACGAAGTT TACCAAAATT 
GAGAAGGAGA AGAGGGAGCA CGCCAAGCAG CACGGGATGA TCCGCACGGA GATCACCGGC 
GCCGAGATCG CGGAGGAAAT GGAGAAGGAG CGGCGCCTCT TCCAGCTCCA GATGTGCGAG 
TATCTCATTA AAGTTAATGA AATCAAGACC AAAAAGGGTG TGGATCTGCT GCAGAACCTG 
ATAAAGTATT ATCACGCACA GTGCAATTTC TTTCAAGATG GTTTGAAAAC AGCTGATAAA 
TTGAAACAGT ACATTGAAAA GCTGGCTGCT GATTTGTATA ATATCAAACA GACCCAGGAC 
GAAGAAAAGA AACAGCTGAC CGCACTCCGA GACCTAATAA AGTCCTCGCT CCAACTCGAT 
CAGAAAGAAT CTAGGAGAGA TTCCCAGAGC CGGCAGGGAG GCTACAGCAT GCACCAGCTG 
CAGGGCAACA AGGAATACGG CAGCGAGAAG AAGGGCTACC TGCTGAAGAA GAGTGACGGG 
ATCCGGAAAG TGTGGCAGAG AAGGAAGTGC TCCGTCAAGA ACGGGATCCT GACCATCTCC 
CACGCCACGT CCAACAGACA GCCAGCCAAG CTGAACCTTC TCACTTGCCA GGTGAAGCCG 
AATGCCGAGG ACAAGAAGTC TTTCGACCTG ATATCACATA ACAGGACGTA TCACTTTCAG 
GCCGAAGATG AGCAGGATTA TGTAGCGTGG ATCTCGGTGC TGACGAACAG CAAAGAGGAG 
GCCCTCACCA TGGCCTTCCG GGGGGAACAG AGTGCTGGGG AGAGCAGCCT GGAGGAGCTG 
ACGAAGGCCA TCATCGAGGA CGTGCAGCGG CTCCCGGGCA ACGACGTCTG CTGCGACTGC 
GGCTCGGCAG AACCCACCTG GCTGTCCACC AACTTGGGTA TCTTGACCTG TATAGAATGT 
TCCGGCATCC ATAGAGAAAT GGGGGTTCAT ATTTCTCGCA TCCAGTCTTT GGAACTAGAC 
AAATTAGGAA CTTCTGAACT CTTGCTGGCC AAGAATGTAG GAAACAATAG TTTTAATGAT 
ATTATGGAAG CAAATTTACC CAGTCCCTCA CCAAAACCCA CCCCTTCAAG TGATATGACC 
GTACGGAAGG AATATATCAC TGCAAAGTAT GTAGATCATA GGTTTTCACG GAAGACCTGT 
TCATCCTCAT CAGCTAAACT GAACGAATTG CTTGAGGCCA TCAAATCCAG GGATTTACTT 
GCACTAATTC AAGTCTATGC AGAGGGGGTG GAGCTAATGG AACCGCTGCT GGAACCCGGA 
CAGGAGCTTG GGGAGACAGC CCTTCATCTT GCAGTCCGAA CCGCAGACCA GACATCTCTC 
CATTTGGTGG ACTTCCTTGT ACAAAACTGT GGGAACCTAG ATAAGCAGAC GGCCCTGGGG 
AACACGGCCC TGCACTACTG TAGTATGTAC AGTAAACCAG AGTGTTTGAA GCTGCTGCTC 
AGGAGCAAGC CCACTGTGGA CGTCGTTAAT CAGGCTGGAG AGACCGCCCT GGACATAGCA 
AAGAGACTGA AAGCCACTCA GTGTGAAGAC CTGCTTTCCC AAGCTAAATC TGGAAAGTTC 
AATCCACACG TCCACGTGGA ATATGAGTGG AATCTTCGAC AGGAGGAGAT GGATGAGAGC 
GATGACGACC TGGATGACAA ACCGAGCCCC ATCAAGAAGG AGCGCTCCCC CCGACCGCAG 
AGCTTCTGCC ACTCCTCCAG CATCTCCCCC CAGGACAAGC TCTCACTGCC GGGCTTCAGC 
ACGCCAAGGG ACAAGCAACG ACTCTCCTAC GGCGCCTTCA CCAACCAGAT CTTCGTCTCC 
ACAAGCACAG ACTCACCCAC GTCACCGATC GCAGAGGCGC CCCCGCTGCC TCCCAGAAAC 
GCCACGAAAG GTAACGACGT GGGTCCCTCA TCGTCCAGTA AGACCACGAA CAAGTTCGAG 
GGCCTGTCCC AGCAGTCGAG CACCGGTTCT GCAAAGACTG CACTTGGCCC AAGAGTTCTT 
CCTAAACTAC CTCAGAAAGT GGCACTAAGG AAAACAGAGA CCAGCCATCA TCTCTCCCTC 
GACAAAGCCA ACGTCCCACC TGAGATCTTC CAGAAGTCGT CCCAGTTGAC AGAGTTACCG 
CAGAAGCCGC CACCCGGGGA CCTGCCCCCG AAGCCCACGG AACTGGCTCC CAAACCCCCC 
ATTGGAGACT TACCACCTAA GCCAGGCGAG CTGCCCCCGA AGCCACAGCT GGGCGACCTG 
CCCCCCAAGC CCCAGCTCGC AGACTTGCCC CCCAAGCCCC AGGTGAAAGA CCTGCCTCCC 
AAGCCACAAC TGGGGGAGCT GCTGGCAAAA CCCCAGACGG GAGACGCCTC GCCCAAGGCC 
CAGCCACCCC TGGAGCTCAC CCCCAAGTCA CACCCGGCGG ACCTGTCCCC GAACGTCCCC 
AAGCAGGCGT CTGAGGACAC CAACGACCTC ACGCCCACCC TGCCAGAGAC ACCTGTGCCG 
CTGCCCAGGA AGATCAACAC GGGGAAGAGC AAGGTGAGGC GAGTGAAGAC CATCTACGAC 
TGCCAGGCGG ACAACGATGA CGAGCTGACT TTCATGGAGG GCGAGGTGAT CGTGGTCACC 
GGGGAGGAGG ACCAGGAGTG GTGGATTGGG CACATCGAGG GGCAGCCCGA GAGGAAGGGC 
GTCTTCCCAG TGTCCTTTGT CCACATCCTG TCGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329896.1
CDS	134..3352
Translation	MRSSASRLSSFSSRDSLWNRMPDQISVSEFIAETTEDYNSPTTSSFTTRLHNCRNTVTLLEEALDQDRTALQKVKKSVKAIYNSGQDHVQNEENYAQVLDKFGSNFLSRDNPDLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGVKGDLKKPFDKAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDQKESRRDSQSRQGGYSMHQLQGNKEYGSEKKGYLLKKSDGIRKVWQRRKCSVKNGILTISHATSNRQPAKLNLLTCQVKPNAEDKKSFDLISHNRTYHFQAEDEQDYVAWISVLTNSKEEALTMAFRGEQSAGESSLEELTKAIIEDVQRLPGNDVCCDCGSAEPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDKLGTSELLLAKNVGNNSFNDIMEANLPSPSPKPTPSSDMTVRKEYITAKYVDHRFSRKTCSSSSAKLNELLEAIKSRDLLALIQVYAEGVELMEPLLEPGQELGETALHLAVRTADQTSLHLVDFLVQNCGNLDKQTALGNTALHYCSMYSKPECLKLLLRSKPTVDVVNQAGETALDIAKRLKATQCEDLLSQAKSGKFNPHVHVEYEWNLRQEEMDESDDDLDDKPSPIKKERSPRPQSFCHSSSISPQDKLSLPGFSTPRDKQRLSYGAFTNQIFVSTSTDSPTSPIAEAPPLPPRNATKGNDVGPSSSSKTTNKFEGLSQQSSTGSAKTALGPRVLPKLPQKVALRKTETSHHLSLDKANVPPEIFQKSSQLTELPQKPPPGDLPPKPTELAPKPPIGDLPPKPGELPPKPQLGDLPPKPQLADLPPKPQVKDLPPKPQLGELLAKPQTGDASPKAQPPLELTPKSHPADLSPNVPKQASEDTNDLTPTLPETPVPLPRKINTGKSKVRRVKTIYDCQADNDDELTFMEGEVIVVTGEEDQEWWIGHIEGQPERKGVFPVSFVHILSD

Target ORF information:

RefSeq Version	XM_015474410.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (ASAP1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474410.1

ORF Insert Sequence:

ATGAGATCTT CAGCCTCCAG GCTCTCCAGT TTTTCATCAA GAGATTCGCT ATGGAATCGG 
ATGCCGGACC AGATCTCCGT CTCCGAGTTC ATCGCCGAGA CCACCGAGGA CTACAACTCG 
CCCACCACGT CCAGCTTCAC TACGCGGCTG CACAACTGCA GGAACACCGT CACGCTGCTG 
GAGGAGGCTC TAGACCAAGA TAGAACAGCC TTACAGAAAG TTAAGAAGTC TGTAAAAGCA 
ATATACAATT CCGGTCAAGA CCATGTACAA AATGAAGAAA ACTATGCACA AGTTCTTGAT 
AAGTTTGGGA GTAATTTTTT AAGTCGAGAC AACCCAGATC TTGGCACCGC TTTTGTCAAG 
TTTTCTACGC TTACAAAGGA ACTGTCCACA CTGCTGAAAA ATCTGCTCCA GGGCCTGAGC 
CACAATGTGA TCTTCACCTT GGATTCCTTG TTGAAAGGAG ACCTGAAGGG AGTCAAAGGC 
GATCTCAAGA AACCATTTGA CAAAGCTTGG AAAGATTATG AGACGAAGTT TACCAAAATT 
GAGAAGGAGA AGAGGGAGCA CGCCAAGCAG CACGGGATGA TCCGCACGGA GATCACCGGC 
GCCGAGATCG CGGAGGAAAT GGAGAAGGAG CGGCGCCTCT TCCAGCTCCA GATGTGCGAG 
TATCTCATTA AAGTTAATGA AATCAAGACC AAAAAGGGTG TGGATCTGCT GCAGAACCTG 
ATAAAGTATT ATCACGCACA GTGCAATTTC TTTCAAGATG GTTTGAAAAC AGCTGATAAA 
TTGAAACAGT ACATTGAAAA GCTGGCTGCT GATTTGTATA ATATCAAACA GACCCAGGAC 
GAAGAAAAGA AACAGCTGAC CGCACTCCGA GACCTAATAA AGTCCTCGCT CCAACTCGAT 
CAGAAAGAAT CTAGGAGAGA TTCCCAGAGC CGGCAGGGAG GCTACAGCAT GCACCAGCTG 
CAGGGCAACA AGGAATACGG CAGCGAGAAG AAGGGCTACC TGCTGAAGAA GAGTGACGGG 
ATCCGGAAAG TGTGGCAGAG AAGGAAGTGC TCCGTCAAGA ACGGGATCCT GACCATCTCC 
CACGCCACGT CCAACAGACA GCCAGCCAAG CTGAACCTTC TCACTTGCCA GGTGAAGCCG 
AATGCCGAGG ACAAGAAGTC TTTCGACCTG ATATCACATA ACAGGACGTA TCACTTTCAG 
GCCGAAGATG AGCAGGATTA TGTAGCGTGG ATCTCGGTGC TGACGAACAG CAAAGAGGAG 
GCCCTCACCA TGGCCTTCCG GGGGGAACAG AGTGCTGGGG AGAGCAGCCT GGAGGAGCTG 
ACGAAGGCCA TCATCGAGGA CGTGCAGCGG CTCCCGGGCA ACGACGTCTG CTGCGACTGC 
GGCTCGGCAG AACCCACCTG GCTGTCCACC AACTTGGGTA TCTTGACCTG TATAGAATGT 
TCCGGCATCC ATAGAGAAAT GGGGGTTCAT ATTTCTCGCA TCCAGTCTTT GGAACTAGAC 
AAATTAGGAA CTTCTGAACT CTTGCTGGCC AAGAATGTAG GAAACAATAG TTTTAATGAT 
ATTATGGAAG CAAATTTACC CAGTCCCTCA CCAAAACCCA CCCCTTCAAG TGATATGACC 
GTACGGAAGG AATATATCAC TGCAAAGTAT GTAGATCATA GGTTTTCACG GAAGACCTGT 
TCATCCTCAT CAGCTAAACT GAACGAATTG CTTGAGGCCA TCAAATCCAG GGATTTACTT 
GCACTAATTC AAGTCTATGC AGAGGGGGTG GAGCTAATGG AACCGCTGCT GGAACCCGGA 
CAGGAGCTTG GGGAGACAGC CCTTCATCTT GCAGTCCGAA CCGCAGACCA GACATCTCTC 
CATTTGGTGG ACTTCCTTGT ACAAAACTGT GGGAACCTAG ATAAGCAGAC GGCCCTGGGG 
AACACGGCCC TGCACTACTG TAGTATGTAC AGTAAACCAG AGTGTTTGAA GCTGCTGCTC 
AGGAGCAAGC CCACTGTGGA CGTCGTTAAT CAGGCTGGAG AGACCGCCCT GGACATAGCA 
AAGAGACTGA AAGCCACTCA GTGTGAAGAC CTGCTTTCCC AAGCTAAATC TGGAAAGTTC 
AATCCACACG TCCACGTGGA ATATGAGTGG AATCTTCGAC AGGAGGAGAT GGATGAGAGC 
GATGACGACC TGGATGACAA ACCGAGCCCC ATCAAGAAGG AGCGCTCCCC CCGACCGCAG 
AGCTTCTGCC ACTCCTCCAG CATCTCCCCC CAGGACAAGC TCTCACTGCC GGGCTTCAGC 
ACGCCAAGGG ACAAGCAACG ACTCTCCTAC GGCGCCTTCA CCAACCAGAT CTTCGTCTCC 
ACAAGCACAG ACTCACCCAC GTCACCGATC GCAGAGGCGC CCCCGCTGCC TCCCAGAAAC 
GCCACGAAAG GTAACGACGT GGGTCCCTCA TCGTCCAGTA AGACCACGAA CAAGTTCGAG 
GGCCTGTCCC AGCAGTCGAG CACCGGTTCT GCAAAGACTG CACTTGGCCC AAGAGTTCTT 
CCTAAACTAC CTCAGAAAGT GGCACTAAGG AAAACAGAGA CCAGCCATCA TCTCTCCCTC 
GACAAAGCCA ACGTCCCACC TGAGATCTTC CAGAAGTCGT CCCAGTTGAC AGAGTTACCG 
CAGAAGCCGC CACCCGGGGA CCTGCCCCCG AAGCCCACGG AACTGGCTCC CAAACCCCCC 
ATTGGAGACT TACCACCTAA GCCAGGCGAG CTGCCCCCGA AGCCACAGCT GGGCGACCTG 
CCCCCCAAGC CCCAGCTCGC AGACTTGCCC CCCAAGCCCC AGGTGAAAGA CCTGCCTCCC 
AAGCCACAAC TGGGGGAGCT GCTGGCAAAA CCCCAGACGG GAGACGCCTC GCCCAAGGCC 
CAGCCACCCC TGGAGCTCAC CCCCAAGTCA CACCCGGCGG ACCTGTCCCC GAACGTCCCC 
AAGCAGGCGT CTGAGGACAC CAACGACCTC ACGCCCACCC TGCCAGAGAC ACCTGTGCCG 
CTGCCCAGGA AGATCAACAC GGGGAAGAGC AAGGTGAGGC GAGTGAAGAC CATCTACGAC 
TGCCAGGCGG ACAACGATGA CGAGCTGACT TTCATGGAGG GCGAGGTGAT CGTGGTCACC 
GGGGAGGAGG ACCAGGAGTG GTGGATTGGG CACATCGAGG GGCAGCCCGA GAGGAAGGGC 
GTCTTCCCAG TGTCCTTTGT CCACATCCTG TCGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

DEF-1, a novel Src SH3 binding protein that promotes adipogenesis in fibroblastic cell lines.
Molecular and cellular biology19(3)2330-7(1999 Mar)
King FJ,Hu E,Harris DF,Sarraf P,Spiegelman BM,Roberts TM

ASAP1, a phospholipid-dependent arf GTPase-activating protein that associates with and is phosphorylated by Src.
Molecular and cellular biology18(12)7038-51(1998 Dec)
Brown MT,Andrade J,Radhakrishna H,Donaldson JG,Cooper JA,Randazzo PA

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ASAP1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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