Asap cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Asap
Entrez Gene ID	35783
Full Name	ArfGAP with SH3 domain, ankyrin repeat and PH domain
Synonyms	ASAP,Asap1,CG14758,CG2226,CG30372,Dmel\CG30372,dAsap
General protein information	Preferred Names ArfGAP with SH3 domain, ankyrin repeat and PH domain Names CG30372 gene product from transcript CG30372-RC ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 Asap-PB Asap-PC Asap-PD CG30372-PB CG30372-PC CG30372-PD
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 44B2-44B2

mRNA and Protein(s)

mRNA	Protein	Name
NM_001014504.3	NP_001014504.1	ArfGAP with SH3 domain, ankyrin repeat and PH domain, isoform B
NM_001299254.1	NP_001286183.1	ArfGAP with SH3 domain, ankyrin repeat and PH domain, isoform D
NM_001169614.2	NP_001163085.1	ArfGAP with SH3 domain, ankyrin repeat and PH domain, isoform C

Pathways from BioSystems

Homologies

	ASAP1	XP_001084688.1
Homo sapiens (human)	ASAP1	NP_060952.2
Gallus gallus (chicken)	ASAP1	XP_425945.4
Drosophila melanogaster (fruit fly)	Asap1	NP_001163085.1
Xenopus tropicalis (tropical clawed frog)	asap1	NP_001090735.1
Pan troglodytes (chimpanzee)	ASAP1	XP_003311959.1
Mus musculus (house mouse)	Asap1	NP_034156.2
Danio rerio (zebrafish)	asap1b	XP_005162660.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007726	XP_001687844.1
Canis lupus familiaris (dog)	ASAP1	XP_005627995.1
Bos taurus (cattle)	ASAP1	NP_787015.1
Rattus norvegicus (Norway rat)	Asap1	NP_001037710.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Asap forms a physical complex with Cindr.
Title: Role for a Cindr-Arf6 axis in patterning emerging epithelia.

The following Asap gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Asap cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa09253	NM_001014504.3 Latest version!	Drosophila melanogaster ArfGAP with SH3 domain, ankyrin repeat and PH domain (Asap), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa12143	NM_001169614.2 Latest version!	Drosophila melanogaster ArfGAP with SH3 domain, ankyrin repeat and PH domain (Asap), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa21704	NM_001299254.1 Latest version!	Drosophila melanogaster ArfGAP with SH3 domain, ankyrin repeat and PH domain (Asap), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Asap ( NM_001014504.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001014504.1 Drosophila melanogaster ArfGAP with SH3 domain, ankyrin repeat and PH domain (Asap), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa09253
Clone ID Related Accession (Same CDS sequence)	NM_001014504.3
Accession Version	NM_001014504.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ArfGAP with SH3 domain, ankyrin repeat and PH domain, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001014504.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGCCAT CCCTGATTGC CGTGTCCGAG TTTGTGGAGG AGACGCGCTC CGATTACAGC 
TCGCCCACCA CCTCCACCTT CGCCTCGAGG ATGCCCGACT GCCGCCACAC GATTGGCGTC 
TTGGAAGAGA GATTGGAGTT CGACCGGGAG GGTCTAACTA AGCTGAAAAA AGCGGTCAAG 
GCTATCCACA ACTCAGGAAA CACCCATGTG GACAATGAGA TGTTTATGGT GCGAGCTCTG 
GAGCGACTGG GCGGCAAGGT TATTGAGCAG GATGAGCCGG ACATCGGCGC CGCGTTCCTT 
AAGTTCAGCG TTGTCACCAA GGAGCTTAGC GCGCTGATGA AGACCCTGAT GCAAAACATC 
AACAACATTG TGATGTTTCC GGTGGACTCA ATGCTGAAGA GCGAGCTGCG GGGCGTGAAG 
GGGGACATGA AGCGGCCATT CGACAAGGCG GCCAAGGACT ACGAGGCAAA GTTCATCAAG 
ATTGAGAAGG AGAAGAAAGC CCAGGCCAAA GAGGCGGGTA TGGTGCGCAC AGAGATCGAC 
GCCGCTGTGG TGGCCGAAGA AATGGAGAAG GAGCGTCGAC TCTACCAACT GCAGACCTGC 
GAGTATTTGC TGAAGTACAA AGACATTAAG ACCAAGACGG GAATCGAGCT GTTGCAGCAC 
CTTATTGAGT ATTATCACGC GTTAAGCAAC TACTTTAAGG ACGGCCTACA GACGATCGAG 
CACTTCGGCA CATACATCGG CGACCTCAGC GAGAAGCTGC ATGAGATCAA GCAGAAGCAA 
GACGAGGATC GTCGCAGTTT GCTGGATCTG CGAACAGTTC TGCGCAGCAC TCCAGATTTT 
GAGCGAGTTG ACAATGTGCC ATCGTCGGAG AGCCGAAGTG GGGGAGCTGG TTACTCCCTC 
CACCAGTTGC AAGGTGACAA ACACCACGGC GTCACCCGGC AAGGCCACCT TCTCAAGAAG 
AGCGAGGGCA AGGTGCGTCG CGTTTGGCAG AAACGACGAT GCCGGGTCAC CAGCGATGGT 
TTTCTTGACA TCTTCCATGC CGACGAGTCG AAGCCGCCAA CACGCGTAAA TCTGCTCACC 
TGCCAGATCA AACCGGTGCC GGATGACAAG CGCGGGTTTG ATCTTATCAG CTACAATCGT 
CCGTATCACT TTCAGGCGGA GGATGAAGGC GATCAGAAGG CATGGATGGC CGTCCTGGTG 
AACTGCAAGG AGAAGGCACT TACCAAGGCC TTCCAGCACG CCAATCCGCA GATGAGTCCA 
AGTCTGGTGG AGCTGCAAAA GACTGTGATC CGATATGTAC AGCTACTGCC GGGCAACGAT 
CGATGCTGTG ACTGTGGATC GCGTAACGAT GTGACCTGGA TCAGTTTAAA CTTTGGCATT 
CTAGTGTGCA TTCAGTGCTC TGGGGTTCAT CGCGATCTGG GCGTCCACCA CTCGCGCATC 
CAGAGTCTTA CGCTAGACAA CCTGACGACG GCTAATCTGC TTATTGCTCG TGCCATGGGC 
AACAGCACGC TGAACGACAT TATGGAGGCG AAGCTGGGAA GGGGCAAGCT TCAGCACGAG 
AGCAGCATGG AAGAGCGTTA CGATTTTATC CGCGCCAAGT ATGTTGCCAA GCGCTATGTC 
ATGCGCACGT GCTCGGATGA CAACGACTTA CGGTGCGACT TAGAGCAGGC TGTGGTCAAC 
GCCGACATGA GTCAGTTGCT CCAGGTGTGG GCGGAAGGAG CGGATCTCAC TTGCTGTCTG 
CCCAGCTCTG ATGCCGGAGA GACGGCCCTC CATCTGGCCG TGCTGCGCGA GATGGGATCT 
ACGCTGCACA TAGTCGACTT TCTCATCCAA AATATGCCGC CTAAAGGTCT TAACAAAGCT 
ACCAATCCGG CCGGTCTGTT GGATGTGACG GGAAAGAATA CGGCGTTGCA TTTGTGCGCT 
CTGCACGATC GGAGGGAGTG CATGAAGCTG CTGCTCCGCT CTGGAGCCGA CTATGAGCTC 
AAGAACTCAC AGAACAAAAC AGCTCTGGAT ATTGCCAAAG AAATGGGACA CAATAGCTGC 
AGGGAGCTTA TTGAATGTGC CATTAAGCGG GAGAAGAGCG CCTTCGACCA CATCAACACT 
GACTGGAATC TGCCAAACGA GGACGGTTCC ACGGATTTCA GCGACGATGA AACAGTCATC 
GATGAGCGCA AATCCCGTTC GCGTCCCCCC AGTTTTGCTG GCGGGGACTC ACCTGTTCTG 
CGCTCTCGTT CCTCTACTTG CGACTCCATA CAGTCCAGTT CTAGTCCAAT CGCCAATTGT 
CCGAGTCGGC AATTCACTCT ACCCTCCGGT CTGCCGAGCT ATACGCATTC TGCAGGGACT 
TCGCCCAAAC AGCACATAAG CGTGGGACAG TATCTGGGCA GTGCTACCAA TGTTGGAGGC 
AATGGACCCG GGAATGGTGG TTCAAGTCCC AGTTCCGCCT CCAGCCAGAG CGTTCGCGCG 
GCGCGAAACA GCTTGAATAT GCAAAGCGAT CTGGGCGGGC ATGTGACAGG TGCTCGGAAA 
TCAACATCTA CGGCCAACAT GAATTCTCTG AAGAAGCGTA CAGCCCCAGC ACCACCCCCC 
GGGACATTGG GCAGCGCTTC GTCCAGCTCT TTTTACGGAA CTTTGCCTCA TCCTCCAAGG 
CATTCGCAAA ACTTCGATGC CAGCGACATT CGAGCCATTA ACCACAAGAA CCAGTCGCTG 
GACGTGGCTT ATGGCACGTT GCCGCACCTT CGGAGCGTAG AAAGCAGTCC CAGGGGCGGA 
GGAGGATATG GGTATGGAGT GTCCCAGGAT CCAGGAGGAT CTGGAAACGG GAGCAACAAC 
AGTCTAATGC CTGCCATGAC AACCTTCGGC CACAAGCGTT CACCCAGCGG TGAGTCATTG 
AACAGGAATA TTCATTTAGC GGGCGCCAAG CTTGTTCTGC CGCCCACCGG TGAGTTGCCC 
ACGCTGAAGC ATGTGGACAA ATCGGCGTTA ACCAGGCCAA AGATTCCTCC ACCAGGACCG 
CCATCAGAGC GCGAAATATC AAACGGACAG TCGAACGAGA GCATCAGTTC CATGGACGAG 
GGACCAGTGG CGCCTCCCCG TAAGCTGGTT AATCAGTCGG CCAATTTTCC CGACTACGAG 
TCCTGGCATA CGGATATGGA CAGCTCTGGT GGCGGTCTAG ATCACTCAGC CGAGTCGAAT 
GTGTCCTCGA GCGACAACGA TCGCCTTAAC TCATCGCCGG ACAATCCGTC GAAAACCGGT 
GGGGCAGGTC TGGGCGGAAA GTTCCACTAT AATGGTCAGC GTCGGTGCCG AGCTCTCTAC 
GATTGCGTGG CGGACAACGA TGATGAGCTG GAGTTCAAGG AAGGCGAGAT TTTGATCGTG 
CTGAACGAGC GCACCGACGA CGAGAACTGG ATGGAGGGCA TTATTGAGGG ACAGCCGACG 
AGGAAGGGCA TGTTTCCCGT CAGTTTCGTG CATATGCTGC CTGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001014504.1
CDS	617..4084
Translation	MPPSLIAVSEFVEETRSDYSSPTTSTFASRMPDCRHTIGVLEERLEFDREGLTKLKKAVKAIHNSGNTHVDNEMFMVRALERLGGKVIEQDEPDIGAAFLKFSVVTKELSALMKTLMQNINNIVMFPVDSMLKSELRGVKGDMKRPFDKAAKDYEAKFIKIEKEKKAQAKEAGMVRTEIDAAVVAEEMEKERRLYQLQTCEYLLKYKDIKTKTGIELLQHLIEYYHALSNYFKDGLQTIEHFGTYIGDLSEKLHEIKQKQDEDRRSLLDLRTVLRSTPDFERVDNVPSSESRSGGAGYSLHQLQGDKHHGVTRQGHLLKKSEGKVRRVWQKRRCRVTSDGFLDIFHADESKPPTRVNLLTCQIKPVPDDKRGFDLISYNRPYHFQAEDEGDQKAWMAVLVNCKEKALTKAFQHANPQMSPSLVELQKTVIRYVQLLPGNDRCCDCGSRNDVTWISLNFGILVCIQCSGVHRDLGVHHSRIQSLTLDNLTTANLLIARAMGNSTLNDIMEAKLGRGKLQHESSMEERYDFIRAKYVAKRYVMRTCSDDNDLRCDLEQAVVNADMSQLLQVWAEGADLTCCLPSSDAGETALHLAVLREMGSTLHIVDFLIQNMPPKGLNKATNPAGLLDVTGKNTALHLCALHDRRECMKLLLRSGADYELKNSQNKTALDIAKEMGHNSCRELIECAIKREKSAFDHINTDWNLPNEDGSTDFSDDETVIDERKSRSRPPSFAGGDSPVLRSRSSTCDSIQSSSSPIANCPSRQFTLPSGLPSYTHSAGTSPKQHISVGQYLGSATNVGGNGPGNGGSSPSSASSQSVRAARNSLNMQSDLGGHVTGARKSTSTANMNSLKKRTAPAPPPGTLGSASSSSFYGTLPHPPRHSQNFDASDIRAINHKNQSLDVAYGTLPHLRSVESSPRGGGGYGYGVSQDPGGSGNGSNNSLMPAMTTFGHKRSPSGESLNRNIHLAGAKLVLPPTGELPTLKHVDKSALTRPKIPPPGPPSEREISNGQSNESISSMDEGPVAPPRKLVNQSANFPDYESWHTDMDSSGGGLDHSAESNVSSSDNDRLNSSPDNPSKTGGAGLGGKFHYNGQRRCRALYDCVADNDDELEFKEGEILIVLNERTDDENWMEGIIEGQPTRKGMFPVSFVHMLPD

Target ORF information:

RefSeq Version	NM_001014504.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ArfGAP with SH3 domain, ankyrin repeat and PH domain (Asap), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001014504.3

ORF Insert Sequence:

ATGCCGCCAT CCCTGATTGC CGTGTCCGAG TTTGTGGAGG AGACGCGCTC CGATTACAGC 
TCGCCCACCA CCTCCACCTT CGCCTCGAGG ATGCCCGACT GCCGCCACAC GATTGGCGTC 
TTGGAAGAGA GATTGGAGTT CGACCGGGAG GGTCTAACTA AGCTGAAAAA AGCGGTCAAG 
GCTATCCACA ACTCAGGAAA CACCCATGTG GACAATGAGA TGTTTATGGT GCGAGCTCTG 
GAGCGACTGG GCGGCAAGGT TATTGAGCAG GATGAGCCGG ACATCGGCGC CGCGTTCCTT 
AAGTTCAGCG TTGTCACCAA GGAGCTTAGC GCGCTGATGA AGACCCTGAT GCAAAACATC 
AACAACATTG TGATGTTTCC GGTGGACTCA ATGCTGAAGA GCGAGCTGCG GGGCGTGAAG 
GGGGACATGA AGCGGCCATT CGACAAGGCG GCCAAGGACT ACGAGGCAAA GTTCATCAAG 
ATTGAGAAGG AGAAGAAAGC CCAGGCCAAA GAGGCGGGTA TGGTGCGCAC AGAGATCGAC 
GCCGCTGTGG TGGCCGAAGA AATGGAGAAG GAGCGTCGAC TCTACCAACT GCAGACCTGC 
GAGTATTTGC TGAAGTACAA AGACATTAAG ACCAAGACGG GAATCGAGCT GTTGCAGCAC 
CTTATTGAGT ATTATCACGC GTTAAGCAAC TACTTTAAGG ACGGCCTACA GACGATCGAG 
CACTTCGGCA CATACATCGG CGACCTCAGC GAGAAGCTGC ATGAGATCAA GCAGAAGCAA 
GACGAGGATC GTCGCAGTTT GCTGGATCTG CGAACAGTTC TGCGCAGCAC TCCAGATTTT 
GAGCGAGTTG ACAATGTGCC ATCGTCGGAG AGCCGAAGTG GGGGAGCTGG TTACTCCCTC 
CACCAGTTGC AAGGTGACAA ACACCACGGC GTCACCCGGC AAGGCCACCT TCTCAAGAAG 
AGCGAGGGCA AGGTGCGTCG CGTTTGGCAG AAACGACGAT GCCGGGTCAC CAGCGATGGT 
TTTCTTGACA TCTTCCATGC CGACGAGTCG AAGCCGCCAA CACGCGTAAA TCTGCTCACC 
TGCCAGATCA AACCGGTGCC GGATGACAAG CGCGGGTTTG ATCTTATCAG CTACAATCGT 
CCGTATCACT TTCAGGCGGA GGATGAAGGC GATCAGAAGG CATGGATGGC CGTCCTGGTG 
AACTGCAAGG AGAAGGCACT TACCAAGGCC TTCCAGCACG CCAATCCGCA GATGAGTCCA 
AGTCTGGTGG AGCTGCAAAA GACTGTGATC CGATATGTAC AGCTACTGCC GGGCAACGAT 
CGATGCTGTG ACTGTGGATC GCGTAACGAT GTGACCTGGA TCAGTTTAAA CTTTGGCATT 
CTAGTGTGCA TTCAGTGCTC TGGGGTTCAT CGCGATCTGG GCGTCCACCA CTCGCGCATC 
CAGAGTCTTA CGCTAGACAA CCTGACGACG GCTAATCTGC TTATTGCTCG TGCCATGGGC 
AACAGCACGC TGAACGACAT TATGGAGGCG AAGCTGGGAA GGGGCAAGCT TCAGCACGAG 
AGCAGCATGG AAGAGCGTTA CGATTTTATC CGCGCCAAGT ATGTTGCCAA GCGCTATGTC 
ATGCGCACGT GCTCGGATGA CAACGACTTA CGGTGCGACT TAGAGCAGGC TGTGGTCAAC 
GCCGACATGA GTCAGTTGCT CCAGGTGTGG GCGGAAGGAG CGGATCTCAC TTGCTGTCTG 
CCCAGCTCTG ATGCCGGAGA GACGGCCCTC CATCTGGCCG TGCTGCGCGA GATGGGATCT 
ACGCTGCACA TAGTCGACTT TCTCATCCAA AATATGCCGC CTAAAGGTCT TAACAAAGCT 
ACCAATCCGG CCGGTCTGTT GGATGTGACG GGAAAGAATA CGGCGTTGCA TTTGTGCGCT 
CTGCACGATC GGAGGGAGTG CATGAAGCTG CTGCTCCGCT CTGGAGCCGA CTATGAGCTC 
AAGAACTCAC AGAACAAAAC AGCTCTGGAT ATTGCCAAAG AAATGGGACA CAATAGCTGC 
AGGGAGCTTA TTGAATGTGC CATTAAGCGG GAGAAGAGCG CCTTCGACCA CATCAACACT 
GACTGGAATC TGCCAAACGA GGACGGTTCC ACGGATTTCA GCGACGATGA AACAGTCATC 
GATGAGCGCA AATCCCGTTC GCGTCCCCCC AGTTTTGCTG GCGGGGACTC ACCTGTTCTG 
CGCTCTCGTT CCTCTACTTG CGACTCCATA CAGTCCAGTT CTAGTCCAAT CGCCAATTGT 
CCGAGTCGGC AATTCACTCT ACCCTCCGGT CTGCCGAGCT ATACGCATTC TGCAGGGACT 
TCGCCCAAAC AGCACATAAG CGTGGGACAG TATCTGGGCA GTGCTACCAA TGTTGGAGGC 
AATGGACCCG GGAATGGTGG TTCAAGTCCC AGTTCCGCCT CCAGCCAGAG CGTTCGCGCG 
GCGCGAAACA GCTTGAATAT GCAAAGCGAT CTGGGCGGGC ATGTGACAGG TGCTCGGAAA 
TCAACATCTA CGGCCAACAT GAATTCTCTG AAGAAGCGTA CAGCCCCAGC ACCACCCCCC 
GGGACATTGG GCAGCGCTTC GTCCAGCTCT TTTTACGGAA CTTTGCCTCA TCCTCCAAGG 
CATTCGCAAA ACTTCGATGC CAGCGACATT CGAGCCATTA ACCACAAGAA CCAGTCGCTG 
GACGTGGCTT ATGGCACGTT GCCGCACCTT CGGAGCGTAG AAAGCAGTCC CAGGGGCGGA 
GGAGGATATG GGTATGGAGT GTCCCAGGAT CCAGGAGGAT CTGGAAACGG GAGCAACAAC 
AGTCTAATGC CTGCCATGAC AACCTTCGGC CACAAGCGTT CACCCAGCGG TGAGTCATTG 
AACAGGAATA TTCATTTAGC GGGCGCCAAG CTTGTTCTGC CGCCCACCGG TGAGTTGCCC 
ACGCTGAAGC ATGTGGACAA ATCGGCGTTA ACCAGGCCAA AGATTCCTCC ACCAGGACCG 
CCATCAGAGC GCGAAATATC AAACGGACAG TCGAACGAGA GCATCAGTTC CATGGACGAG 
GGACCAGTGG CGCCTCCCCG TAAGCTGGTT AATCAGTCGG CCAATTTTCC CGACTACGAG 
TCCTGGCATA CGGATATGGA CAGCTCTGGT GGCGGTCTAG ATCACTCAGC CGAGTCGAAT 
GTGTCCTCGA GCGACAACGA TCGCCTTAAC TCATCGCCGG ACAATCCGTC GAAAACCGGT 
GGGGCAGGTC TGGGCGGAAA GTTCCACTAT AATGGTCAGC GTCGGTGCCG AGCTCTCTAC 
GATTGCGTGG CGGACAACGA TGATGAGCTG GAGTTCAAGG AAGGCGAGAT TTTGATCGTG 
CTGAACGAGC GCACCGACGA CGAGAACTGG ATGGAGGGCA TTATTGAGGG ACAGCCGACG 
AGGAAGGGCA TGTTTCCCGT CAGTTTCGTG CATATGCTGC CTGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asap ( NM_001169614.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163085.1 Drosophila melanogaster ArfGAP with SH3 domain, ankyrin repeat and PH domain (Asap), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa12143
Clone ID Related Accession (Same CDS sequence)	NM_001169614.2
Accession Version	NM_001169614.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3384bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ArfGAP with SH3 domain, ankyrin repeat and PH domain, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001169614.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGCCAT CCCTGATTGC CGTGTCCGAG TTTGTGGAGG AGACGCGCTC CGATTACAGC 
TCGCCCACCA CCTCCACCTT CGCCTCGAGG ATGCCCGACT GCCGCCACAC GATTGGCGTC 
TTGGAAGAGA GATTGGAGTT CGACCGGGAG GGTCTAACTA AGCTGAAAAA AGCGGTCAAG 
GCTATCCACA ACTCAGGAAA CACCCATGTG GACAATGAGA TGTTTATGGT GCGAGCTCTG 
GAGCGACTGG GCGGCAAGGT TATTGAGCAG GATGAGCCGG ACATCGGCGC CGCGTTCCTT 
AAGTTCAGCG TTGTCACCAA GGAGCTTAGC GCGCTGATGA AGACCCTGAT GCAAAACATC 
AACAACATTG TGATGTTTCC GGTGGACTCA ATGCTGAAGA GCGAGCTGCG GGGCGTGAAG 
GGGGACATGA AGCGGCCATT CGACAAGGCG GCCAAGGACT ACGAGGCAAA GTTCATCAAG 
ATTGAGAAGG AGAAGAAAGC CCAGGCCAAA GAGGCGGGTA TGGTGCGCAC AGAGATCGAC 
GCCGCTGTGG TGGCCGAAGA AATGGAGAAG GAGCGTCGAC TCTACCAACT GCAGACCTGC 
GAGTATTTGC TGAAGTACAA AGACATTAAG ACCAAGACGG GAATCGAGCT GTTGCAGCAC 
CTTATTGAGT ATTATCACGC GTTAAGCAAC TACTTTAAGG ACGGCCTACA GACGATCGAG 
CACTTCGGCA CATACATCGG CGACCTCAGC GAGAAGCTGC ATGAGATCAA GCAGAAGCAA 
GACGAGGATC GTCGCAGTTT GCTGGATCTG CGAACAGTTC TGCGCAGCAC TCCAGATTTT 
GAGCGAGTTG ACAATGTGCC ATCGTCGGAG AGCCGAAGTG GGGGAGCTGG TTACTCCCTC 
CACCAGTTGC AAGGTGACAA ACACCACGGC GTCACCCGGC AAGGCCACCT TCTCAAGAAG 
AGCGAGGGCA AGGTGCGTCG CGTTTGGCAG AAACGACGAT GCCGGGTCAC CAGCGATGGT 
TTTCTTGACA TCTTCCATGC CGACGAGTCG AAGCCGCCAA CACGCGTAAA TCTGCTCACC 
TGCCAGATCA AACCGGTGCC GGATGACAAG CGCGGGTTTG ATCTTATCAG CTACAATCGT 
CCGTATCACT TTCAGGCGGA GGATGAAGGC GATCAGAAGG CATGGATGGC CGTCCTGGTG 
AACTGCAAGG AGAAGGCACT TACCAAGGCC TTCCAGCACG CCAATCCGCA GATGAGTCCA 
AGTCTGGTGG AGCTGCAAAA GACTGTGATC CGATATGTAC AGCTACTGCC GGGCAACGAT 
CGATGCTGTG ACTGTGGATC GCGTAACGAT GTGACCTGGA TCAGTTTAAA CTTTGGCATT 
CTAGTGTGCA TTCAGTGCTC TGGGGTTCAT CGCGATCTGG GCGTCCACCA CTCGCGCATC 
CAGAGTCTTA CGCTAGACAA CCTGACGACG GCTAATCTGC TTATTGCTCG TGCCATGGGC 
AACAGCACGC TGAACGACAT TATGGAGGCG AAGCTGGGAA GGGGCAAGCT TCAGCACGAG 
AGCAGCATGG AAGAGCGTTA CGATTTTATC CGCGCCAAGT ATGTTGCCAA GCGCTATGTC 
ATGCGCACGT GCTCGGATGA CAACGACTTA CGGTGCGACT TAGAGCAGGC TGTGGTCAAC 
GCCGACATGA GTCAGTTGCT CCAGGTGTGG GCGGAAGGAG CGGATCTCAC TTGCTGTCTG 
CCCAGCTCTG ATGCCGGAGA GACGGCCCTC CATCTGGCCG TGCTGCGCGA GATGGGATCT 
ACGCTGCACA TAGTCGACTT TCTCATCCAA AATATGCCGC CTAAAGGTCT TAACAAAGCT 
ACCAATCCGG CCGGTCTGTT GGATGTGACG GGAAAGAATA CGGCGTTGCA TTTGTGCGCT 
CTGCACGATC GGAGGGAGTG CATGAAGCTG CTGCTCCGCT CTGGAGCCGA CTATGAGCTC 
AAGAACTCAC AGAACAAAAC AGCTCTGGAT ATTGCCAAAG AAATGGGACA CAATAGCTGC 
AGGGAGCTTA TTGAATGTGC CATTAAGCGG GAGAAGAGCG CCTTCGACCA CATCAACACT 
GACTGGAATC TGCCAAACGA GGACGGTTCC ACGGATTTCA GCGACGATGA AACAGTCATC 
GATGAGCGCT CCAGTTCTAG TCCAATCGCC AATTGTCCGA GTCGGCAATT CACTCTACCC 
TCCGGTCTGC CGAGCTATAC GCATTCTGCA GGGACTTCGC CCAAACAGCA CATAAGCGTG 
GGACAGTATC TGGGCAGTGC TACCAATGTT GGAGGCAATG GACCCGGGAA TGGTGGTTCA 
AGTCCCAGTT CCGCCTCCAG CCAGAGCGTT CGCGCGGCGC GAAACAGCTT GAATATGCAA 
AGCGATCTGG GCGGGCATGT GACAGGTGCT CGGAAATCAA CATCTACGGC CAACATGAAT 
TCTCTGAAGA AGCGTACAGC CCCAGCACCA CCCCCCGGGA CATTGGGCAG CGCTTCGTCC 
AGCTCTTTTT ACGGAACTTT GCCTCATCCT CCAAGGCATT CGCAAAACTT CGATGCCAGC 
GACATTCGAG CCATTAACCA CAAGAACCAG TCGCTGGACG TGGCTTATGG CACGTTGCCG 
CACCTTCGGA GCGTAGAAAG CAGTCCCAGG GGCGGAGGAG GATATGGGTA TGGAGTGTCC 
CAGGATCCAG GAGGATCTGG AAACGGGAGC AACAACAGTC TAATGCCTGC CATGACAACC 
TTCGGCCACA AGCGTTCACC CAGCGGTGAG TCATTGAACA GGAATATTCA TTTAGCGGGC 
GCCAAGCTTG TTCTGCCGCC CACCGGTGAG TTGCCCACGC TGAAGCATGT GGACAAATCG 
GCGTTAACCA GGCCAAAGAT TCCTCCACCA GGACCGCCAT CAGAGCGCGA AATATCAAAC 
GGACAGTCGA ACGAGAGCAT CAGTTCCATG GACGAGGGAC CAGTGGCGCC TCCCCGTAAG 
CTGGTTAATC AGTCGGCCAA TTTTCCCGAC TACGAGTCCT GGCATACGGA TATGGACAGC 
TCTGGTGGCG GTCTAGATCA CTCAGCCGAG TCGAATGTGT CCTCGAGCGA CAACGATCGC 
CTTAACTCAT CGCCGGACAA TCCGTCGAAA ACCGGTGGGG CAGGTCTGGG CGGAAAGTTC 
CACTATAATG GTCAGCGTCG GTGCCGAGCT CTCTACGATT GCGTGGCGGA CAACGATGAT 
GAGCTGGAGT TCAAGGAAGG CGAGATTTTG ATCGTGCTGA ACGAGCGCAC CGACGACGAG 
AACTGGATGG AGGGCATTAT TGAGGGACAG CCGACGAGGA AGGGCATGTT TCCCGTCAGT 
TTCGTGCATA TGCTGCCTGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163085.1
CDS	617..4000
Translation	MPPSLIAVSEFVEETRSDYSSPTTSTFASRMPDCRHTIGVLEERLEFDREGLTKLKKAVKAIHNSGNTHVDNEMFMVRALERLGGKVIEQDEPDIGAAFLKFSVVTKELSALMKTLMQNINNIVMFPVDSMLKSELRGVKGDMKRPFDKAAKDYEAKFIKIEKEKKAQAKEAGMVRTEIDAAVVAEEMEKERRLYQLQTCEYLLKYKDIKTKTGIELLQHLIEYYHALSNYFKDGLQTIEHFGTYIGDLSEKLHEIKQKQDEDRRSLLDLRTVLRSTPDFERVDNVPSSESRSGGAGYSLHQLQGDKHHGVTRQGHLLKKSEGKVRRVWQKRRCRVTSDGFLDIFHADESKPPTRVNLLTCQIKPVPDDKRGFDLISYNRPYHFQAEDEGDQKAWMAVLVNCKEKALTKAFQHANPQMSPSLVELQKTVIRYVQLLPGNDRCCDCGSRNDVTWISLNFGILVCIQCSGVHRDLGVHHSRIQSLTLDNLTTANLLIARAMGNSTLNDIMEAKLGRGKLQHESSMEERYDFIRAKYVAKRYVMRTCSDDNDLRCDLEQAVVNADMSQLLQVWAEGADLTCCLPSSDAGETALHLAVLREMGSTLHIVDFLIQNMPPKGLNKATNPAGLLDVTGKNTALHLCALHDRRECMKLLLRSGADYELKNSQNKTALDIAKEMGHNSCRELIECAIKREKSAFDHINTDWNLPNEDGSTDFSDDETVIDERSSSSPIANCPSRQFTLPSGLPSYTHSAGTSPKQHISVGQYLGSATNVGGNGPGNGGSSPSSASSQSVRAARNSLNMQSDLGGHVTGARKSTSTANMNSLKKRTAPAPPPGTLGSASSSSFYGTLPHPPRHSQNFDASDIRAINHKNQSLDVAYGTLPHLRSVESSPRGGGGYGYGVSQDPGGSGNGSNNSLMPAMTTFGHKRSPSGESLNRNIHLAGAKLVLPPTGELPTLKHVDKSALTRPKIPPPGPPSEREISNGQSNESISSMDEGPVAPPRKLVNQSANFPDYESWHTDMDSSGGGLDHSAESNVSSSDNDRLNSSPDNPSKTGGAGLGGKFHYNGQRRCRALYDCVADNDDELEFKEGEILIVLNERTDDENWMEGIIEGQPTRKGMFPVSFVHMLPD

Target ORF information:

RefSeq Version	NM_001169614.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ArfGAP with SH3 domain, ankyrin repeat and PH domain (Asap), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169614.2

ORF Insert Sequence:

ATGCCGCCAT CCCTGATTGC CGTGTCCGAG TTTGTGGAGG AGACGCGCTC CGATTACAGC 
TCGCCCACCA CCTCCACCTT CGCCTCGAGG ATGCCCGACT GCCGCCACAC GATTGGCGTC 
TTGGAAGAGA GATTGGAGTT CGACCGGGAG GGTCTAACTA AGCTGAAAAA AGCGGTCAAG 
GCTATCCACA ACTCAGGAAA CACCCATGTG GACAATGAGA TGTTTATGGT GCGAGCTCTG 
GAGCGACTGG GCGGCAAGGT TATTGAGCAG GATGAGCCGG ACATCGGCGC CGCGTTCCTT 
AAGTTCAGCG TTGTCACCAA GGAGCTTAGC GCGCTGATGA AGACCCTGAT GCAAAACATC 
AACAACATTG TGATGTTTCC GGTGGACTCA ATGCTGAAGA GCGAGCTGCG GGGCGTGAAG 
GGGGACATGA AGCGGCCATT CGACAAGGCG GCCAAGGACT ACGAGGCAAA GTTCATCAAG 
ATTGAGAAGG AGAAGAAAGC CCAGGCCAAA GAGGCGGGTA TGGTGCGCAC AGAGATCGAC 
GCCGCTGTGG TGGCCGAAGA AATGGAGAAG GAGCGTCGAC TCTACCAACT GCAGACCTGC 
GAGTATTTGC TGAAGTACAA AGACATTAAG ACCAAGACGG GAATCGAGCT GTTGCAGCAC 
CTTATTGAGT ATTATCACGC GTTAAGCAAC TACTTTAAGG ACGGCCTACA GACGATCGAG 
CACTTCGGCA CATACATCGG CGACCTCAGC GAGAAGCTGC ATGAGATCAA GCAGAAGCAA 
GACGAGGATC GTCGCAGTTT GCTGGATCTG CGAACAGTTC TGCGCAGCAC TCCAGATTTT 
GAGCGAGTTG ACAATGTGCC ATCGTCGGAG AGCCGAAGTG GGGGAGCTGG TTACTCCCTC 
CACCAGTTGC AAGGTGACAA ACACCACGGC GTCACCCGGC AAGGCCACCT TCTCAAGAAG 
AGCGAGGGCA AGGTGCGTCG CGTTTGGCAG AAACGACGAT GCCGGGTCAC CAGCGATGGT 
TTTCTTGACA TCTTCCATGC CGACGAGTCG AAGCCGCCAA CACGCGTAAA TCTGCTCACC 
TGCCAGATCA AACCGGTGCC GGATGACAAG CGCGGGTTTG ATCTTATCAG CTACAATCGT 
CCGTATCACT TTCAGGCGGA GGATGAAGGC GATCAGAAGG CATGGATGGC CGTCCTGGTG 
AACTGCAAGG AGAAGGCACT TACCAAGGCC TTCCAGCACG CCAATCCGCA GATGAGTCCA 
AGTCTGGTGG AGCTGCAAAA GACTGTGATC CGATATGTAC AGCTACTGCC GGGCAACGAT 
CGATGCTGTG ACTGTGGATC GCGTAACGAT GTGACCTGGA TCAGTTTAAA CTTTGGCATT 
CTAGTGTGCA TTCAGTGCTC TGGGGTTCAT CGCGATCTGG GCGTCCACCA CTCGCGCATC 
CAGAGTCTTA CGCTAGACAA CCTGACGACG GCTAATCTGC TTATTGCTCG TGCCATGGGC 
AACAGCACGC TGAACGACAT TATGGAGGCG AAGCTGGGAA GGGGCAAGCT TCAGCACGAG 
AGCAGCATGG AAGAGCGTTA CGATTTTATC CGCGCCAAGT ATGTTGCCAA GCGCTATGTC 
ATGCGCACGT GCTCGGATGA CAACGACTTA CGGTGCGACT TAGAGCAGGC TGTGGTCAAC 
GCCGACATGA GTCAGTTGCT CCAGGTGTGG GCGGAAGGAG CGGATCTCAC TTGCTGTCTG 
CCCAGCTCTG ATGCCGGAGA GACGGCCCTC CATCTGGCCG TGCTGCGCGA GATGGGATCT 
ACGCTGCACA TAGTCGACTT TCTCATCCAA AATATGCCGC CTAAAGGTCT TAACAAAGCT 
ACCAATCCGG CCGGTCTGTT GGATGTGACG GGAAAGAATA CGGCGTTGCA TTTGTGCGCT 
CTGCACGATC GGAGGGAGTG CATGAAGCTG CTGCTCCGCT CTGGAGCCGA CTATGAGCTC 
AAGAACTCAC AGAACAAAAC AGCTCTGGAT ATTGCCAAAG AAATGGGACA CAATAGCTGC 
AGGGAGCTTA TTGAATGTGC CATTAAGCGG GAGAAGAGCG CCTTCGACCA CATCAACACT 
GACTGGAATC TGCCAAACGA GGACGGTTCC ACGGATTTCA GCGACGATGA AACAGTCATC 
GATGAGCGCT CCAGTTCTAG TCCAATCGCC AATTGTCCGA GTCGGCAATT CACTCTACCC 
TCCGGTCTGC CGAGCTATAC GCATTCTGCA GGGACTTCGC CCAAACAGCA CATAAGCGTG 
GGACAGTATC TGGGCAGTGC TACCAATGTT GGAGGCAATG GACCCGGGAA TGGTGGTTCA 
AGTCCCAGTT CCGCCTCCAG CCAGAGCGTT CGCGCGGCGC GAAACAGCTT GAATATGCAA 
AGCGATCTGG GCGGGCATGT GACAGGTGCT CGGAAATCAA CATCTACGGC CAACATGAAT 
TCTCTGAAGA AGCGTACAGC CCCAGCACCA CCCCCCGGGA CATTGGGCAG CGCTTCGTCC 
AGCTCTTTTT ACGGAACTTT GCCTCATCCT CCAAGGCATT CGCAAAACTT CGATGCCAGC 
GACATTCGAG CCATTAACCA CAAGAACCAG TCGCTGGACG TGGCTTATGG CACGTTGCCG 
CACCTTCGGA GCGTAGAAAG CAGTCCCAGG GGCGGAGGAG GATATGGGTA TGGAGTGTCC 
CAGGATCCAG GAGGATCTGG AAACGGGAGC AACAACAGTC TAATGCCTGC CATGACAACC 
TTCGGCCACA AGCGTTCACC CAGCGGTGAG TCATTGAACA GGAATATTCA TTTAGCGGGC 
GCCAAGCTTG TTCTGCCGCC CACCGGTGAG TTGCCCACGC TGAAGCATGT GGACAAATCG 
GCGTTAACCA GGCCAAAGAT TCCTCCACCA GGACCGCCAT CAGAGCGCGA AATATCAAAC 
GGACAGTCGA ACGAGAGCAT CAGTTCCATG GACGAGGGAC CAGTGGCGCC TCCCCGTAAG 
CTGGTTAATC AGTCGGCCAA TTTTCCCGAC TACGAGTCCT GGCATACGGA TATGGACAGC 
TCTGGTGGCG GTCTAGATCA CTCAGCCGAG TCGAATGTGT CCTCGAGCGA CAACGATCGC 
CTTAACTCAT CGCCGGACAA TCCGTCGAAA ACCGGTGGGG CAGGTCTGGG CGGAAAGTTC 
CACTATAATG GTCAGCGTCG GTGCCGAGCT CTCTACGATT GCGTGGCGGA CAACGATGAT 
GAGCTGGAGT TCAAGGAAGG CGAGATTTTG ATCGTGCTGA ACGAGCGCAC CGACGACGAG 
AACTGGATGG AGGGCATTAT TGAGGGACAG CCGACGAGGA AGGGCATGTT TCCCGTCAGT 
TTCGTGCATA TGCTGCCTGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asap ( NM_001299254.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286183.1 Drosophila melanogaster ArfGAP with SH3 domain, ankyrin repeat and PH domain (Asap), transcript variant D, mRNA.

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CloneID	OFa21704
Clone ID Related Accession (Same CDS sequence)	NM_001299254.1
Accession Version	NM_001299254.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3432bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ArfGAP with SH3 domain, ankyrin repeat and PH domain, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGCCAT CCCTGATTGC CGTGTCCGAG TTTGTGGAGG AGACGCGCTC CGATTACAGC 
TCGCCCACCA CCTCCACCTT CGCCTCGAGG ATGCCCGACT GCCGCCACAC GATTGGCGTC 
TTGGAAGAGA GATTGGAGTT CGACCGGGAG GGTCTAACTA AGCTGAAAAA AGCGGTCAAG 
GCTATCCACA ACTCAGGAAA CACCCATGTG GACAATGAGA TGTTTATGGT GCGAGCTCTG 
GAGCGACTGG GCGGCAAGGT TATTGAGCAG GATGAGCCGG ACATCGGCGC CGCGTTCCTT 
AAGTTCAGCG TTGTCACCAA GGAGCTTAGC GCGCTGATGA AGACCCTGAT GCAAAACATC 
AACAACATTG TGATGTTTCC GGTGGACTCA ATGCTGAAGA GCGAGCTGCG GGGCGTGAAG 
GGGGACATGA AGCGGCCATT CGACAAGGCG GCCAAGGACT ACGAGGCAAA GTTCATCAAG 
ATTGAGAAGG AGAAGAAAGC CCAGGCCAAA GAGGCGGGTA TGGTGCGCAC AGAGATCGAC 
GCCGCTGTGG TGGCCGAAGA AATGGAGAAG GAGCGTCGAC TCTACCAACT GCAGACCTGC 
GAGTATTTGC TGAAGTACAA AGACATTAAG ACCAAGACGG GAATCGAGCT GTTGCAGCAC 
CTTATTGAGT ATTATCACGC GTTAAGCAAC TACTTTAAGG ACGGCCTACA GACGATCGAG 
CACTTCGGCA CATACATCGG CGACCTCAGC GAGAAGCTGC ATGAGATCAA GCAGAAGCAA 
GACGAGGATC GTCGCAGTTT GCTGGATCTG CGAACAGTTC TGCGCAGCAC TCCAGATTTT 
GAGCGAGTTG ACAATGTGCC ATCGTCGGAG AGCCGAAGTG GGGGAGCTGG TTACTCCCTC 
CACCAGTTGC AAGGTGACAA ACACCACGGC GTCACCCGGC AAGGCCACCT TCTCAAGAAG 
AGCGAGGGCA AGGTGCGTCG CGTTTGGCAG AAACGACGAT GCCGGGTCAC CAGCGATGGT 
TTTCTTGACA TCTTCCATGC CGACGAGTCG AAGCCGCCAA CACGCGTAAA TCTGCTCACC 
TGCCAGATCA AACCGGTGCC GGATGACAAG CGCGGGTTTG ATCTTATCAG CTACAATCGT 
CCGTATCACT TTCAGGCGGA GGATGAAGGC GATCAGAAGG CATGGATGGC CGTCCTGGTG 
AACTGCAAGG AGAAGGCACT TACCAAGGCC TTCCAGCACG CCAATCCGCA GATGAGTCCA 
AGTCTGGTGG AGCTGCAAAA GACTGTGATC CGATATGTAC AGCTACTGCC GGGCAACGAT 
CGATGCTGTG ACTGTGGATC GCGTAACGAT GTGACCTGGA TCAGTTTAAA CTTTGGCATT 
CTAGTGTGCA TTCAGTGCTC TGGGGTTCAT CGCGATCTGG GCGTCCACCA CTCGCGCATC 
CAGAGTCTTA CGCTAGACAA CCTGACGACG GCTAATCTGC TTATTGCTCG TGCCATGGGC 
AACAGCACGC TGAACGACAT TATGGAGGCG AAGCTGGGAA GGGGCAAGCT TCAGCACGAG 
AGCAGCATGG AAGAGCGTTA CGATTTTATC CGCGCCAAGT ATGTTGCCAA GCGCTATGTC 
ATGCGCACGT GCTCGGATGA CAACGACTTA CGGTGCGACT TAGAGCAGGC TGTGGTCAAC 
GCCGACATGA GTCAGTTGCT CCAGGTGTGG GCGGAAGGAG CGGATCTCAC TTGCTGTCTG 
CCCAGCTCTG ATGCCGGAGA GACGGCCCTC CATCTGGCCG TGCTGCGCGA GATGGGATCT 
ACGCTGCACA TAGTCGACTT TCTCATCCAA AATATGCCGC CTAAAGGTCT TAACAAAGCT 
ACCAATCCGG CCGGTCTGTT GGATGTGACG GGAAAGAATA CGGCGTTGCA TTTGTGCGCT 
CTGCACGATC GGAGGGAGTG CATGAAGCTG CTGCTCCGCT CTGGAGCCGA CTATGAGCTC 
AAGAACTCAC AGAACAAAAC AGCTCTGGAT ATTGCCAAAG AAATGGGACA CAATAGCTGC 
AGGGAGCTTA TTGAATGTGC CATTAAGCGG GAGAAGAGCG CCTTCGACCA CATCAACACT 
GACTGGAATC TGCCAAACGA GGACGGTTCC ACGGATTTCA GCGACGATGA AACAGTCATC 
GATGAGCGCT CCAGTTCTAG TCCAATCGCC AATTGTCCGA GTCGGCAATT CACTCTACCC 
TCCGGTCTGC CGAGCTATAC GCATTCTGCA GGGACTTCGC CCAAACAGCA CATAAGCGTG 
GGACAGTATC TGGGCAGTGC TACCAATGTT GGAGGCAATG GACCCGGGAA TGGTGGTTCA 
AGTCCCAGTT CCGCCTCCAG CCAGAGCGTT CGCGCGGCGC GAAACAGCTT GAATATGCAA 
AGCGATCTGG GCGGGCATGT GACAGGTGCT CGGAAATCAA CATCTACGGC CAACATGAAT 
TCTCTGAAGA AGCGTACAGC CCCAGCACCA CCCCCCGGGA CATTGGGCAG CGCTTCGTCC 
AGCTCTTTTT ACGGAACTTT GCCTCATCCT CCAAGGCATT CGCAAAACTT CGATGCCAGC 
GACATTCGAG CCATTAACCA CAAGAACCAG TCGCTGGACG TGGCTTATGG CACGTTGCCG 
CACCTTCGGA GCGTAGAAAG CAGTCCCAGG GGCGGAGGAG GATATGGGTA TGGAGTGTCC 
CAGGATCCAG GAGGATCTGG AAACGGGAGC AACAACAGTC TAATGCCTGC CATGACAACC 
TTCGGCCACA AGCGTTCACC CAGCGGTGAG TCATTGAACA GGAATATTCA TTTAGCGGGC 
GCCAAGCTTG TTCTGCCGCC CACCGGTGAG TTGCCCACGC TGAAGCATGT GGACAAATCG 
GCGTTAACCA GGCCAAAGAT TCCTCCACCA GGACCGCCAT CAGAGCGCGA AATATCAAAC 
GGACAGTCGA ACGAGAGCAT CAGTTCCATG GACGAGGGAC CAGTGGCGCC TCCCCGTAAG 
CGCAAGCAAC ACATCACTCA ATTGAGATGC CAGTCCAGCG AGGAGACGCT GGTTAATCAG 
TCGGCCAATT TTCCCGACTA CGAGTCCTGG CATACGGATA TGGACAGCTC TGGTGGCGGT 
CTAGATCACT CAGCCGAGTC GAATGTGTCC TCGAGCGACA ACGATCGCCT TAACTCATCG 
CCGGACAATC CGTCGAAAAC CGGTGGGGCA GGTCTGGGCG GAAAGTTCCA CTATAATGGT 
CAGCGTCGGT GCCGAGCTCT CTACGATTGC GTGGCGGACA ACGATGATGA GCTGGAGTTC 
AAGGAAGGCG AGATTTTGAT CGTGCTGAAC GAGCGCACCG ACGACGAGAA CTGGATGGAG 
GGCATTATTG AGGGACAGCC GACGAGGAAG GGCATGTTTC CCGTCAGTTT CGTGCATATG 
CTGCCTGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286183.1
CDS	617..4048
Translation	MPPSLIAVSEFVEETRSDYSSPTTSTFASRMPDCRHTIGVLEERLEFDREGLTKLKKAVKAIHNSGNTHVDNEMFMVRALERLGGKVIEQDEPDIGAAFLKFSVVTKELSALMKTLMQNINNIVMFPVDSMLKSELRGVKGDMKRPFDKAAKDYEAKFIKIEKEKKAQAKEAGMVRTEIDAAVVAEEMEKERRLYQLQTCEYLLKYKDIKTKTGIELLQHLIEYYHALSNYFKDGLQTIEHFGTYIGDLSEKLHEIKQKQDEDRRSLLDLRTVLRSTPDFERVDNVPSSESRSGGAGYSLHQLQGDKHHGVTRQGHLLKKSEGKVRRVWQKRRCRVTSDGFLDIFHADESKPPTRVNLLTCQIKPVPDDKRGFDLISYNRPYHFQAEDEGDQKAWMAVLVNCKEKALTKAFQHANPQMSPSLVELQKTVIRYVQLLPGNDRCCDCGSRNDVTWISLNFGILVCIQCSGVHRDLGVHHSRIQSLTLDNLTTANLLIARAMGNSTLNDIMEAKLGRGKLQHESSMEERYDFIRAKYVAKRYVMRTCSDDNDLRCDLEQAVVNADMSQLLQVWAEGADLTCCLPSSDAGETALHLAVLREMGSTLHIVDFLIQNMPPKGLNKATNPAGLLDVTGKNTALHLCALHDRRECMKLLLRSGADYELKNSQNKTALDIAKEMGHNSCRELIECAIKREKSAFDHINTDWNLPNEDGSTDFSDDETVIDERSSSSPIANCPSRQFTLPSGLPSYTHSAGTSPKQHISVGQYLGSATNVGGNGPGNGGSSPSSASSQSVRAARNSLNMQSDLGGHVTGARKSTSTANMNSLKKRTAPAPPPGTLGSASSSSFYGTLPHPPRHSQNFDASDIRAINHKNQSLDVAYGTLPHLRSVESSPRGGGGYGYGVSQDPGGSGNGSNNSLMPAMTTFGHKRSPSGESLNRNIHLAGAKLVLPPTGELPTLKHVDKSALTRPKIPPPGPPSEREISNGQSNESISSMDEGPVAPPRKRKQHITQLRCQSSEETLVNQSANFPDYESWHTDMDSSGGGLDHSAESNVSSSDNDRLNSSPDNPSKTGGAGLGGKFHYNGQRRCRALYDCVADNDDELEFKEGEILIVLNERTDDENWMEGIIEGQPTRKGMFPVSFVHMLPD

Target ORF information:

RefSeq Version	NM_001299254.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ArfGAP with SH3 domain, ankyrin repeat and PH domain (Asap), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299254.1

ORF Insert Sequence:

ATGCCGCCAT CCCTGATTGC CGTGTCCGAG TTTGTGGAGG AGACGCGCTC CGATTACAGC 
TCGCCCACCA CCTCCACCTT CGCCTCGAGG ATGCCCGACT GCCGCCACAC GATTGGCGTC 
TTGGAAGAGA GATTGGAGTT CGACCGGGAG GGTCTAACTA AGCTGAAAAA AGCGGTCAAG 
GCTATCCACA ACTCAGGAAA CACCCATGTG GACAATGAGA TGTTTATGGT GCGAGCTCTG 
GAGCGACTGG GCGGCAAGGT TATTGAGCAG GATGAGCCGG ACATCGGCGC CGCGTTCCTT 
AAGTTCAGCG TTGTCACCAA GGAGCTTAGC GCGCTGATGA AGACCCTGAT GCAAAACATC 
AACAACATTG TGATGTTTCC GGTGGACTCA ATGCTGAAGA GCGAGCTGCG GGGCGTGAAG 
GGGGACATGA AGCGGCCATT CGACAAGGCG GCCAAGGACT ACGAGGCAAA GTTCATCAAG 
ATTGAGAAGG AGAAGAAAGC CCAGGCCAAA GAGGCGGGTA TGGTGCGCAC AGAGATCGAC 
GCCGCTGTGG TGGCCGAAGA AATGGAGAAG GAGCGTCGAC TCTACCAACT GCAGACCTGC 
GAGTATTTGC TGAAGTACAA AGACATTAAG ACCAAGACGG GAATCGAGCT GTTGCAGCAC 
CTTATTGAGT ATTATCACGC GTTAAGCAAC TACTTTAAGG ACGGCCTACA GACGATCGAG 
CACTTCGGCA CATACATCGG CGACCTCAGC GAGAAGCTGC ATGAGATCAA GCAGAAGCAA 
GACGAGGATC GTCGCAGTTT GCTGGATCTG CGAACAGTTC TGCGCAGCAC TCCAGATTTT 
GAGCGAGTTG ACAATGTGCC ATCGTCGGAG AGCCGAAGTG GGGGAGCTGG TTACTCCCTC 
CACCAGTTGC AAGGTGACAA ACACCACGGC GTCACCCGGC AAGGCCACCT TCTCAAGAAG 
AGCGAGGGCA AGGTGCGTCG CGTTTGGCAG AAACGACGAT GCCGGGTCAC CAGCGATGGT 
TTTCTTGACA TCTTCCATGC CGACGAGTCG AAGCCGCCAA CACGCGTAAA TCTGCTCACC 
TGCCAGATCA AACCGGTGCC GGATGACAAG CGCGGGTTTG ATCTTATCAG CTACAATCGT 
CCGTATCACT TTCAGGCGGA GGATGAAGGC GATCAGAAGG CATGGATGGC CGTCCTGGTG 
AACTGCAAGG AGAAGGCACT TACCAAGGCC TTCCAGCACG CCAATCCGCA GATGAGTCCA 
AGTCTGGTGG AGCTGCAAAA GACTGTGATC CGATATGTAC AGCTACTGCC GGGCAACGAT 
CGATGCTGTG ACTGTGGATC GCGTAACGAT GTGACCTGGA TCAGTTTAAA CTTTGGCATT 
CTAGTGTGCA TTCAGTGCTC TGGGGTTCAT CGCGATCTGG GCGTCCACCA CTCGCGCATC 
CAGAGTCTTA CGCTAGACAA CCTGACGACG GCTAATCTGC TTATTGCTCG TGCCATGGGC 
AACAGCACGC TGAACGACAT TATGGAGGCG AAGCTGGGAA GGGGCAAGCT TCAGCACGAG 
AGCAGCATGG AAGAGCGTTA CGATTTTATC CGCGCCAAGT ATGTTGCCAA GCGCTATGTC 
ATGCGCACGT GCTCGGATGA CAACGACTTA CGGTGCGACT TAGAGCAGGC TGTGGTCAAC 
GCCGACATGA GTCAGTTGCT CCAGGTGTGG GCGGAAGGAG CGGATCTCAC TTGCTGTCTG 
CCCAGCTCTG ATGCCGGAGA GACGGCCCTC CATCTGGCCG TGCTGCGCGA GATGGGATCT 
ACGCTGCACA TAGTCGACTT TCTCATCCAA AATATGCCGC CTAAAGGTCT TAACAAAGCT 
ACCAATCCGG CCGGTCTGTT GGATGTGACG GGAAAGAATA CGGCGTTGCA TTTGTGCGCT 
CTGCACGATC GGAGGGAGTG CATGAAGCTG CTGCTCCGCT CTGGAGCCGA CTATGAGCTC 
AAGAACTCAC AGAACAAAAC AGCTCTGGAT ATTGCCAAAG AAATGGGACA CAATAGCTGC 
AGGGAGCTTA TTGAATGTGC CATTAAGCGG GAGAAGAGCG CCTTCGACCA CATCAACACT 
GACTGGAATC TGCCAAACGA GGACGGTTCC ACGGATTTCA GCGACGATGA AACAGTCATC 
GATGAGCGCT CCAGTTCTAG TCCAATCGCC AATTGTCCGA GTCGGCAATT CACTCTACCC 
TCCGGTCTGC CGAGCTATAC GCATTCTGCA GGGACTTCGC CCAAACAGCA CATAAGCGTG 
GGACAGTATC TGGGCAGTGC TACCAATGTT GGAGGCAATG GACCCGGGAA TGGTGGTTCA 
AGTCCCAGTT CCGCCTCCAG CCAGAGCGTT CGCGCGGCGC GAAACAGCTT GAATATGCAA 
AGCGATCTGG GCGGGCATGT GACAGGTGCT CGGAAATCAA CATCTACGGC CAACATGAAT 
TCTCTGAAGA AGCGTACAGC CCCAGCACCA CCCCCCGGGA CATTGGGCAG CGCTTCGTCC 
AGCTCTTTTT ACGGAACTTT GCCTCATCCT CCAAGGCATT CGCAAAACTT CGATGCCAGC 
GACATTCGAG CCATTAACCA CAAGAACCAG TCGCTGGACG TGGCTTATGG CACGTTGCCG 
CACCTTCGGA GCGTAGAAAG CAGTCCCAGG GGCGGAGGAG GATATGGGTA TGGAGTGTCC 
CAGGATCCAG GAGGATCTGG AAACGGGAGC AACAACAGTC TAATGCCTGC CATGACAACC 
TTCGGCCACA AGCGTTCACC CAGCGGTGAG TCATTGAACA GGAATATTCA TTTAGCGGGC 
GCCAAGCTTG TTCTGCCGCC CACCGGTGAG TTGCCCACGC TGAAGCATGT GGACAAATCG 
GCGTTAACCA GGCCAAAGAT TCCTCCACCA GGACCGCCAT CAGAGCGCGA AATATCAAAC 
GGACAGTCGA ACGAGAGCAT CAGTTCCATG GACGAGGGAC CAGTGGCGCC TCCCCGTAAG 
CGCAAGCAAC ACATCACTCA ATTGAGATGC CAGTCCAGCG AGGAGACGCT GGTTAATCAG 
TCGGCCAATT TTCCCGACTA CGAGTCCTGG CATACGGATA TGGACAGCTC TGGTGGCGGT 
CTAGATCACT CAGCCGAGTC GAATGTGTCC TCGAGCGACA ACGATCGCCT TAACTCATCG 
CCGGACAATC CGTCGAAAAC CGGTGGGGCA GGTCTGGGCG GAAAGTTCCA CTATAATGGT 
CAGCGTCGGT GCCGAGCTCT CTACGATTGC GTGGCGGACA ACGATGATGA GCTGGAGTTC 
AAGGAAGGCG AGATTTTGAT CGTGCTGAAC GAGCGCACCG ACGACGAGAA CTGGATGGAG 
GGCATTATTG AGGGACAGCC GACGAGGAAG GGCATGTTTC CCGTCAGTTT CGTGCATATG 
CTGCCTGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Asap cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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