EEA1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	EEA1
Entrez Gene ID	8411
Full Name	early endosome antigen 1
Synonyms	MST105,MSTP105,ZFYVE2
General protein information	Preferred Names early endosome antigen 1 Names early endosome antigen 1 early endosome antigen 1, 162kD early endosome-associated protein endosome-associated protein p162 zinc finger FYVE domain-containing protein 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q22
Disorder MIM:	605070

mRNA and Protein(s)

mRNA	Protein	Name
NM_003566.3	NP_003557.2	early endosome antigen 1
XM_011538814.2	XP_011537116.1	early endosome antigen 1 isoform X1
XM_017020018.1	XP_016875507.1	early endosome antigen 1 isoform X2
XM_011538815.2	XP_011537117.1	early endosome antigen 1 isoform X3
NM_003566.4	NP_003557.3	early endosome antigen 1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Eea1	NP_001101556.1
Canis lupus familiaris (dog)	EEA1	XP_005629490.1
Gallus gallus (chicken)	EEA1	XP_004937680.1
Xenopus tropicalis (tropical clawed frog)	eea1	XP_002935361.1
Homo sapiens (human)	EEA1	NP_003557.2
	EEA1	XP_001104577.1
Mus musculus (house mouse)	Eea1	NP_001001932.1
Pan troglodytes (chimpanzee)	EEA1	XP_522610.2
Bos taurus (cattle)	EEA1	XP_005206182.1
Danio rerio (zebrafish)	LOC562943	XP_003200485.1

Gene Ontology
Bibliography

Related articles in PubMed

An endosomal tether undergoes an entropic collapse to bring vesicles together.
Murray DH, Jahnel M, Lauer J, Avellaneda MJ, Brouilly N, Cezanne A, Morales-Navarrete H, Perini ED, Ferguson C, Lupas AN, Kalaidzidis Y, Parton RG, Grill SW, Zerial M
Nature537(7618)107-111(2016 09)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

results suggest a new mechanism in which Rab5 induces a change in flexibility of EEA1, generating an entropic collapse force that pulls the captured vesicle towards the target membrane to initiate docking and membrane fusion
Title: An endosomal tether undergoes an entropic collapse to bring vesicles together.

EEA1 mobility on endosomes is decreased upon stimulation of EGF receptor endocytosis in HeLa cells
Title: Mobility of tethering factor EEA1 on endosomes is decreased upon stimulation of EGF receptor endocytosis in HeLa cells.

Under the regulation of Rab5, the fused vesicles are targeted to early endosomes and thus deliver the internalized TbetaRI to the caveolin-1 and EEA1 double-positive early endosomes (caveolin-1-positive early endosomes).
Title: Internalization of the TGF-? type I receptor into caveolin-1 and EEA1 double-positive early endosomes.

Data indicate that early endosome antigen 1 (EEA1) is important for the small GTPase Rab31-mediated enhancement of ligand-bound EGF receptor (EGFR) endocytic trafficking.
Title: Engagement of the small GTPase Rab31 protein and its effector, early endosome antigen 1, is important for trafficking of the ligand-bound epidermal growth factor receptor from the early to the late endosome.

In serum-deprivated HeLa cells with low endocytic activity there two types of EEA1-vesicles: the first one carries the both EEA1 and Rab5 at high levels; the second consists of weakly decorated EEA1-vesicles that can be both Rab5-positive and -negative.
Title: [Analysis of vesicle subpopulations carrying early endosomal autoantigen EEA1].

The following EEA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EEA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu03394	NM_003566.3 Latest version!	Homo sapiens early endosome antigen 1 (EEA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu108733	NM_003566.4 Latest version!	Homo sapiens early endosome antigen 1 (EEA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu84196	XM_017020018.1 Latest version!	Homo sapiens early endosome antigen 1 (EEA1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu61745	XM_011538814.2 Latest version!	Homo sapiens early endosome antigen 1 (EEA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu61746	XM_011538815.2 Latest version!	Homo sapiens early endosome antigen 1 (EEA1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EEA1 ( NM_003566.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_003557.2 Homo sapiens early endosome antigen 1 (EEA1), mRNA.

Price & Availability↑

CloneID	OHu03394
Clone ID Related Accession (Same CDS sequence)	NM_003566.3
Accession Version	NM_003566.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4236bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Homo sapiens(human)
Product	early endosome antigen 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BP228986.1, X78998.1, AC016136.21 and BF515615.1. On Jul 7, 2006 this sequence version replaced NM_003566.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.232910.1, SRR1660807.4023.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTAAGGA GGATTTTACA GAGGACTCCT GGGAGAGTTG GCTCTCAAGG TTCTGATTTA 
GATTCATCAG CAACTCCTAT AAACACAGTG GACGTCAATA ATGAAAGCTC TTCAGAGGGT 
TTCATATGTC CCCAGTGTAT GAAATCTCTT GGATCTGCTG ATGAACTTTT CAAACATTAT 
GAAGCTGTTC ATGATGCTGG TAATGACTCA GGTCATGGAG GAGAGTCTAA TCTTGCTTTG 
AAGCGAGATG ATGTAACACT GCTCAGACAA GAGGTCCAAG ACCTACAGGC TTCACTTAAG 
GAAGAAAAAT GGTACTCGGA AGAATTAAAG AAGGAATTAG AAAAATATCA AGGGCTGCAG 
CAGCAAGAGG CCAAACCTGA TGGGTTGGTG ACTGATTCAT CAGCAGAACT ACAGTCTTTG 
GAACAGCAAT TAGAAGAAGC CCAAACAGAA AATTTTAATA TTAAGCAAAT GAAAGACTTA 
TTTGAACAGA AAGCAGCCCA ACTTGCTACT GAAATTGCAG ATATAAAGTC AAAGTATGAT 
GAAGAAAGGA GTCTTCGAGA AGCTGCTGAA CAAAAAGTGA CACGTCTGAC AGAAGAATTA 
AACAAAGAGG CAACTGTAAT TCAAGATCTG AAGACGGAAC TGCTTCAGAG ACCTGGTATA 
GAAGATGTTG CCGTGCTAAA GAAAGAACTG GTCCAAGTTC AAACACTAAT GGATAACATG 
ACCTTGGAAC GTGAGCGAGA ATCTGAAAAA CTCAAAGATG AATGCAAAAA ATTGCAGTCA 
CAATATGCTA GCTCAGAGGC CACAATAAGC CAGCTAAGGA GTGAACTTGC CAAAGGCCCC 
CAGGAAGTTG CTGTATATGT ACAGGAACTA CAAAAACTGA AAAGTTCAGT TAATGAATTA 
ACACAAAAAA ATCAGACCTT GACAGAAAAC TTGCTGAAAA AAGAACAAGA CTATACTAAG 
TTAGAGGAGA AACATAATGA AGAATCTGTG AGTAAAAAGA ATATTCAGGC AACCCTTCAT 
CAAAAAGACC TAGATTGTCA ACAGCTTCAG TCAAGATTGT CTGCATCTGA AACCTCACTG 
CATAGAATAC ATGTAGAACT AAGTGAAAAA GGAGAAGCTA CTCAAAAGCT CAAAGAAGAA 
TTATCTGAGG TAGAGACCAA GTACCAGCAT CTAAAGGCGG AGTTTAAGCA GCTACAACAA 
CAGAGAGAAG AAAAGGAGCA GCATGGGTTA CAACTCCAAA GTGAAATTAA TCAATTACAT 
AGCAAACTTC TGGAGACAGA GCGCCAACTA GGGGAAGCTC ATGGTAGGCT GAAGGAACAG 
AGACAGCTTT CAAGTGAAAA GTTGATGGAT AAAGAACAAC AAGTGGCTGA TTTACAACTC 
AAACTTTCTC GGTTAGAAGA GCAGTTGAAG GAAAAAGTTA CAAATTCTAC AGAATTGCAG 
CATCAATTAG ATAAAACAAA GCAACAGCAT CAAGAACAAC AGGCTCTTCA GCAAAGCACC 
ACGGCAAAAC TTCGAGAAGC TCAGAATGAT TTGGAACAAG TTCTACGTCA AATTGGCGAT 
AAGGACCAAA AGATCCAGAA CCTTGAAGCT TTATTACAGA AGAGTAAAGA AAATATTTCA 
TTACTAGAAA AAGAAAGAGA AGATCTTTAT GCAAAAATTC AGGCTGGTGA AGGAGAGACT 
GCTGTTCTTA ACCAGTTACA AGAAAAAAAC CATACACTAC AGGAGCAAGT AACTCAACTA 
ACAGAGAAGC TGAAGAATCA GTCAGAAAGT CATAAACAAG CCCAGGAGAA TTTGCATGAC 
CAGGTACAAG AGCAGAAGGC ACATCTTAGA GCTGCACAAG ACCGTGTCCT TTCCCTAGAA 
ACTAGTGTCA ATGAATTAAA TAGTCAATTA AATGAAAGCA AGGAGAAGGT CTCCCAGCTT 
GACATACAGA TTAAAGCCAA AACCGAACTA TTACTATCAG CAGAAGCAGC AAAAACTGCT 
CAAAGAGCTG ATCTTCAGAA TCATTTGGAC ACAGCTCAAA ATGCATTACA AGATAAACAG 
CAGGAGTTAA ATAAGATTAC TACTCAGTTG GATCAGGTCA CTGCAAAGTT ACAAGACAAG 
CAAGAACATT GCAGTCAGCT GGAAAGTCAT CTTAAAGAAT ATAAAGAGAA ATACCTCTCT 
TTAGAACAGA AAACCGAAGA GCTAGAAGGT CAAATTAAGA AACTAGAAGC TGATAGTCTT 
GAAGTTAAAG CAAGCAAGGA GCAGGCTTTG CAAGATCTAC AACAGCAAAG ACAGCTGAAC 
ACAGATTTAG AGCTCAGAGC CACAGAATTG AGTAAACAAC TTGAAATGGA GAAGGAAATA 
GTATCCAGTA CAAGATTGGA TCTACAGAAA AAATCTGAAG CCCTTGAAAG TATCAAGCAA 
AAGCTTACCA AGCAAGAGGA AGAAAAACAA ATCCTGAAAC AAGATTTTGA AACTTTAAGT 
CAAGAAACAA AGATTCAGCA TGAGGAATTG AATAACAGAA TTCAAACAAC AGTAACAGAA 
CTACAAAAAG TGAAAATGGA GAAAGAAGCT TTAATGACAG AGCTTTCTAC AGTAAAGGAC 
AAACTATCAA AAGTTTCTGA TTCTTTGAAA AACTCTAAAA GTGAATTTGA AAAGGAGAAT 
CAGAAAGGAA AAGCCGCTAT ATTAGACTTG GAAAAAACTT GCAAAGAATT AAAGCATCAA 
CTTCAAGTGC AGATGGAAAA CACACTTAAG GAACAGAAGG AACTGAAAAA GTCACTTGAA 
AAAGAGAAGG AGGCTTCTCA TCAGTTGAAA TTGGAACTCA ATTCAATGCA GGAACAACTT 
ATACAGGCCC AGAATACTTT AAAACAAAAT GAAAAAGAAG AGCAACAACT TCAGGGGAAC 
ATAAATGAGC TAAAGCAATC AAGTGAACAG AAGAAAAAAC AAATTGAAGC ACTCCAAGGA 
GAGCTTAAAA TTGCTGTTTT ACAGAAGACA GAGCTTGAGA ATAAACTACA GCAGCAGTTA 
ACACAGGCAG CCCAGGAACT TGCAGCAGAG AAAGAGAAAA TATCAGTATT ACAAAACAAC 
TATGAAAAAA GTCAGGAAAC TTTCAAACAG CTTCAATCTG ATTTCTATGG GAGGGAATCT 
GAACTTCTAG CCACCAGGCA AGATCTTAAG TCTGTAGAAG AGAAGCTTTC TCTAGCACAG 
GAGGACTTGA TTTCAAACAG AAATCAAATT GGAAATCAAA ATAAATTGAT TCAAGAACTG 
AAGACTGCCA AGGCTACATT GGAGCAGGAT TCAGCAAAGA AAGAACAGCA ATTGCAGGAG 
CGATGTAAAG CACTACAAGA CATTCAGAAA GAAAAGTCAC TGAAAGAAAA AGAACTGGTA 
AATGAGAAGT CTAAATTGGC AGAGATAGAA GAAATTAAAT GTAGACAAGA AAAAGAAATC 
ACTAAACTAA ACGAAGAACT CAAGTCCCAC AAACTAGAAA GCATAAAGGA GATAACAAAT 
CTTAAAGATG CTAAACAGCT TCTAATTCAG CAGAAATTAG AACTTCAAGG AAAAGCGGAC 
TCCCTGAAGG CAGCTGTTGA ACAGGAGAAG AGAAATCAGC AGATACTAAA AGACCAGGTG 
AAAAAGGAAG AAGAGGAGCT GAAGAAAGAA TTTATTGAGA AAGAAGCTAA GTTGCATTCC 
GAAATAAAAG AAAAGGAAGT AGGAATGAAG AAGCATGAAG AAAATGAGGC TAAACTTACC 
ATGCAGATTA CAGCATTAAA TGAAAACTTA GGCACTGTGA AGAAGGAGTG GCAATCTAGT 
CAACGGAGAG TTAGTGAGCT TGAGAAACAA ACGGATGACT TACGGGGTGA AATTGCAGTA 
TTAGAAGCAA CGGTTCAGAA TAATCAAGAT GAAAGGAGAG CACTACTGGA AAGATGTCTT 
AAAGGAGAAG GTGAAATAGA AAAGCTTCAA ACCAAAGTAT TAGAATTGCA AAGAAAGCTG 
GATAATACAA CTGCAGCAGT GCAGGAGCTG GGCAGAGAAA ACCAATCACT TCAGATCAAA 
CATACACAAG CGTTGAATAG AAAGTGGGCC GAAGACAATG AAGTACAAAA CTGTATGGCC 
TGTGGGAAAG GCTTTTCAGT AACAGTGAGA CGGCATCACT GCCGACAGTG TGGAAATATC 
TTCTGTGCTG AATGTTCAGC CAAAAATGCC TTAACTCCTT CCTCCAAGAA GCCTGTTCGT 
GTCTGTGATG CATGTTTCAA TGACTTGCAA GGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003557.2
CDS	266..4501
Translation	MLRRILQRTPGRVGSQGSDLDSSATPINTVDVNNESSSEGFICPQCMKSLGSADELFKHYEAVHDAGNDSGHGGESNLALKRDDVTLLRQEVQDLQASLKEEKWYSEELKKELEKYQGLQQQEAKPDGLVTDSSAELQSLEQQLEEAQTENFNIKQMKDLFEQKAAQLATEIADIKSKYDEERSLREAAEQKVTRLTEELNKEATVIQDLKTELLQRPGIEDVAVLKKELVQVQTLMDNMTLERERESEKLKDECKKLQSQYASSEATISQLRSELAKGPQEVAVYVQELQKLKSSVNELTQKNQTLTENLLKKEQDYTKLEEKHNEESVSKKNIQATLHQKDLDCQQLQSRLSASETSLHRIHVELSEKGEATQKLKEELSEVETKYQHLKAEFKQLQQQREEKEQHGLQLQSEINQLHSKLLETERQLGEAHGRLKEQRQLSSEKLMDKEQQVADLQLKLSRLEEQLKEKVTNSTELQHQLDKTKQQHQEQQALQQSTTAKLREAQNDLEQVLRQIGDKDQKIQNLEALLQKSKENISLLEKEREDLYAKIQAGEGETAVLNQLQEKNHTLQEQVTQLTEKLKNQSESHKQAQENLHDQVQEQKAHLRAAQDRVLSLETSVNELNSQLNESKEKVSQLDIQIKAKTELLLSAEAAKTAQRADLQNHLDTAQNALQDKQQELNKITTQLDQVTAKLQDKQEHCSQLESHLKEYKEKYLSLEQKTEELEGQIKKLEADSLEVKASKEQALQDLQQQRQLNTDLELRATELSKQLEMEKEIVSSTRLDLQKKSEALESIKQKLTKQEEEKQILKQDFETLSQETKIQHEELNNRIQTTVTELQKVKMEKEALMTELSTVKDKLSKVSDSLKNSKSEFEKENQKGKAAILDLEKTCKELKHQLQVQMENTLKEQKELKKSLEKEKEASHQLKLELNSMQEQLIQAQNTLKQNEKEEQQLQGNINELKQSSEQKKKQIEALQGELKIAVLQKTELENKLQQQLTQAAQELAAEKEKISVLQNNYEKSQETFKQLQSDFYGRESELLATRQDLKSVEEKLSLAQEDLISNRNQIGNQNKLIQELKTAKATLEQDSAKKEQQLQERCKALQDIQKEKSLKEKELVNEKSKLAEIEEIKCRQEKEITKLNEELKSHKLESIKEITNLKDAKQLLIQQKLELQGKADSLKAAVEQEKRNQQILKDQVKKEEEELKKEFIEKEAKLHSEIKEKEVGMKKHEENEAKLTMQITALNENLGTVKKEWQSSQRRVSELEKQTDDLRGEIAVLEATVQNNQDERRALLERCLKGEGEIEKLQTKVLELQRKLDNTTAAVQELGRENQSLQIKHTQALNRKWAEDNEVQNCMACGKGFSVTVRRHHCRQCGNIFCAECSAKNALTPSSKKPVRVCDACFNDLQG

Target ORF information:

RefSeq Version	NM_003566.3
Organism	Homo sapiens(human)
Definition	Homo sapiens early endosome antigen 1 (EEA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003566.3

ORF Insert Sequence:

ATGTTAAGGA GGATTTTACA GAGGACTCCT GGGAGAGTTG GCTCTCAAGG TTCTGATTTA 
GATTCATCAG CAACTCCTAT AAACACAGTG GACGTCAATA ATGAAAGCTC TTCAGAGGGT 
TTCATATGTC CCCAGTGTAT GAAATCTCTT GGATCTGCTG ATGAACTTTT CAAACATTAT 
GAAGCTGTTC ATGATGCTGG TAATGACTCA GGTCATGGAG GAGAGTCTAA TCTTGCTTTG 
AAGCGAGATG ATGTAACACT GCTCAGACAA GAGGTCCAAG ACCTACAGGC TTCACTTAAG 
GAAGAAAAAT GGTACTCGGA AGAATTAAAG AAGGAATTAG AAAAATATCA AGGGCTGCAG 
CAGCAAGAGG CCAAACCTGA TGGGTTGGTG ACTGATTCAT CAGCAGAACT ACAGTCTTTG 
GAACAGCAAT TAGAAGAAGC CCAAACAGAA AATTTTAATA TTAAGCAAAT GAAAGACTTA 
TTTGAACAGA AAGCAGCCCA ACTTGCTACT GAAATTGCAG ATATAAAGTC AAAGTATGAT 
GAAGAAAGGA GTCTTCGAGA AGCTGCTGAA CAAAAAGTGA CACGTCTGAC AGAAGAATTA 
AACAAAGAGG CAACTGTAAT TCAAGATCTG AAGACGGAAC TGCTTCAGAG ACCTGGTATA 
GAAGATGTTG CCGTGCTAAA GAAAGAACTG GTCCAAGTTC AAACACTAAT GGATAACATG 
ACCTTGGAAC GTGAGCGAGA ATCTGAAAAA CTCAAAGATG AATGCAAAAA ATTGCAGTCA 
CAATATGCTA GCTCAGAGGC CACAATAAGC CAGCTAAGGA GTGAACTTGC CAAAGGCCCC 
CAGGAAGTTG CTGTATATGT ACAGGAACTA CAAAAACTGA AAAGTTCAGT TAATGAATTA 
ACACAAAAAA ATCAGACCTT GACAGAAAAC TTGCTGAAAA AAGAACAAGA CTATACTAAG 
TTAGAGGAGA AACATAATGA AGAATCTGTG AGTAAAAAGA ATATTCAGGC AACCCTTCAT 
CAAAAAGACC TAGATTGTCA ACAGCTTCAG TCAAGATTGT CTGCATCTGA AACCTCACTG 
CATAGAATAC ATGTAGAACT AAGTGAAAAA GGAGAAGCTA CTCAAAAGCT CAAAGAAGAA 
TTATCTGAGG TAGAGACCAA GTACCAGCAT CTAAAGGCGG AGTTTAAGCA GCTACAACAA 
CAGAGAGAAG AAAAGGAGCA GCATGGGTTA CAACTCCAAA GTGAAATTAA TCAATTACAT 
AGCAAACTTC TGGAGACAGA GCGCCAACTA GGGGAAGCTC ATGGTAGGCT GAAGGAACAG 
AGACAGCTTT CAAGTGAAAA GTTGATGGAT AAAGAACAAC AAGTGGCTGA TTTACAACTC 
AAACTTTCTC GGTTAGAAGA GCAGTTGAAG GAAAAAGTTA CAAATTCTAC AGAATTGCAG 
CATCAATTAG ATAAAACAAA GCAACAGCAT CAAGAACAAC AGGCTCTTCA GCAAAGCACC 
ACGGCAAAAC TTCGAGAAGC TCAGAATGAT TTGGAACAAG TTCTACGTCA AATTGGCGAT 
AAGGACCAAA AGATCCAGAA CCTTGAAGCT TTATTACAGA AGAGTAAAGA AAATATTTCA 
TTACTAGAAA AAGAAAGAGA AGATCTTTAT GCAAAAATTC AGGCTGGTGA AGGAGAGACT 
GCTGTTCTTA ACCAGTTACA AGAAAAAAAC CATACACTAC AGGAGCAAGT AACTCAACTA 
ACAGAGAAGC TGAAGAATCA GTCAGAAAGT CATAAACAAG CCCAGGAGAA TTTGCATGAC 
CAGGTACAAG AGCAGAAGGC ACATCTTAGA GCTGCACAAG ACCGTGTCCT TTCCCTAGAA 
ACTAGTGTCA ATGAATTAAA TAGTCAATTA AATGAAAGCA AGGAGAAGGT CTCCCAGCTT 
GACATACAGA TTAAAGCCAA AACCGAACTA TTACTATCAG CAGAAGCAGC AAAAACTGCT 
CAAAGAGCTG ATCTTCAGAA TCATTTGGAC ACAGCTCAAA ATGCATTACA AGATAAACAG 
CAGGAGTTAA ATAAGATTAC TACTCAGTTG GATCAGGTCA CTGCAAAGTT ACAAGACAAG 
CAAGAACATT GCAGTCAGCT GGAAAGTCAT CTTAAAGAAT ATAAAGAGAA ATACCTCTCT 
TTAGAACAGA AAACCGAAGA GCTAGAAGGT CAAATTAAGA AACTAGAAGC TGATAGTCTT 
GAAGTTAAAG CAAGCAAGGA GCAGGCTTTG CAAGATCTAC AACAGCAAAG ACAGCTGAAC 
ACAGATTTAG AGCTCAGAGC CACAGAATTG AGTAAACAAC TTGAAATGGA GAAGGAAATA 
GTATCCAGTA CAAGATTGGA TCTACAGAAA AAATCTGAAG CCCTTGAAAG TATCAAGCAA 
AAGCTTACCA AGCAAGAGGA AGAAAAACAA ATCCTGAAAC AAGATTTTGA AACTTTAAGT 
CAAGAAACAA AGATTCAGCA TGAGGAATTG AATAACAGAA TTCAAACAAC AGTAACAGAA 
CTACAAAAAG TGAAAATGGA GAAAGAAGCT TTAATGACAG AGCTTTCTAC AGTAAAGGAC 
AAACTATCAA AAGTTTCTGA TTCTTTGAAA AACTCTAAAA GTGAATTTGA AAAGGAGAAT 
CAGAAAGGAA AAGCCGCTAT ATTAGACTTG GAAAAAACTT GCAAAGAATT AAAGCATCAA 
CTTCAAGTGC AGATGGAAAA CACACTTAAG GAACAGAAGG AACTGAAAAA GTCACTTGAA 
AAAGAGAAGG AGGCTTCTCA TCAGTTGAAA TTGGAACTCA ATTCAATGCA GGAACAACTT 
ATACAGGCCC AGAATACTTT AAAACAAAAT GAAAAAGAAG AGCAACAACT TCAGGGGAAC 
ATAAATGAGC TAAAGCAATC AAGTGAACAG AAGAAAAAAC AAATTGAAGC ACTCCAAGGA 
GAGCTTAAAA TTGCTGTTTT ACAGAAGACA GAGCTTGAGA ATAAACTACA GCAGCAGTTA 
ACACAGGCAG CCCAGGAACT TGCAGCAGAG AAAGAGAAAA TATCAGTATT ACAAAACAAC 
TATGAAAAAA GTCAGGAAAC TTTCAAACAG CTTCAATCTG ATTTCTATGG GAGGGAATCT 
GAACTTCTAG CCACCAGGCA AGATCTTAAG TCTGTAGAAG AGAAGCTTTC TCTAGCACAG 
GAGGACTTGA TTTCAAACAG AAATCAAATT GGAAATCAAA ATAAATTGAT TCAAGAACTG 
AAGACTGCCA AGGCTACATT GGAGCAGGAT TCAGCAAAGA AAGAACAGCA ATTGCAGGAG 
CGATGTAAAG CACTACAAGA CATTCAGAAA GAAAAGTCAC TGAAAGAAAA AGAACTGGTA 
AATGAGAAGT CTAAATTGGC AGAGATAGAA GAAATTAAAT GTAGACAAGA AAAAGAAATC 
ACTAAACTAA ACGAAGAACT CAAGTCCCAC AAACTAGAAA GCATAAAGGA GATAACAAAT 
CTTAAAGATG CTAAACAGCT TCTAATTCAG CAGAAATTAG AACTTCAAGG AAAAGCGGAC 
TCCCTGAAGG CAGCTGTTGA ACAGGAGAAG AGAAATCAGC AGATACTAAA AGACCAGGTG 
AAAAAGGAAG AAGAGGAGCT GAAGAAAGAA TTTATTGAGA AAGAAGCTAA GTTGCATTCC 
GAAATAAAAG AAAAGGAAGT AGGAATGAAG AAGCATGAAG AAAATGAGGC TAAACTTACC 
ATGCAGATTA CAGCATTAAA TGAAAACTTA GGCACTGTGA AGAAGGAGTG GCAATCTAGT 
CAACGGAGAG TTAGTGAGCT TGAGAAACAA ACGGATGACT TACGGGGTGA AATTGCAGTA 
TTAGAAGCAA CGGTTCAGAA TAATCAAGAT GAAAGGAGAG CACTACTGGA AAGATGTCTT 
AAAGGAGAAG GTGAAATAGA AAAGCTTCAA ACCAAAGTAT TAGAATTGCA AAGAAAGCTG 
GATAATACAA CTGCAGCAGT GCAGGAGCTG GGCAGAGAAA ACCAATCACT TCAGATCAAA 
CATACACAAG CGTTGAATAG AAAGTGGGCC GAAGACAATG AAGTACAAAA CTGTATGGCC 
TGTGGGAAAG GCTTTTCAGT AACAGTGAGA CGGCATCACT GCCGACAGTG TGGAAATATC 
TTCTGTGCTG AATGTTCAGC CAAAAATGCC TTAACTCCTT CCTCCAAGAA GCCTGTTCGT 
GTCTGTGATG CATGTTTCAA TGACTTGCAA GGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EEA1 ( NM_003566.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_003557.3 Homo sapiens early endosome antigen 1 (EEA1), mRNA.

Price & Availability↑

CloneID	OHu108733
Clone ID Related Accession (Same CDS sequence)	NM_003566.4
Accession Version	NM_003566.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4236bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-20
Organism	Homo sapiens(human)
Product	early endosome antigen 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC021646.35, AC026111.31 and AC016136.21. On May 17, 2019 this sequence version replaced NM_003566.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.232910.1, SRR1660807.4023.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000322349.13/ ENSP00000317955.8 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTTAAGGA GGATTTTACA GAGGACTCCT GGGAGAGTTG GCTCTCAAGG TTCTGATTTA 
GATTCATCAG CAACTCCTAT AAACACAGTG GACGTCAATA ATGAAAGCTC TTCAGAGGGT 
TTCATATGTC CCCAGTGTAT GAAATCTCTT GGATCTGCTG ATGAACTTTT CAAACATTAT 
GAAGCTGTTC ATGATGCTGG TAATGACTCA GGTCATGGAG GAGAGTCTAA TCTTGCTTTG 
AAGCGAGATG ATGTAACACT GCTCAGACAA GAGGTCCAAG ACCTACAGGC TTCACTTAAG 
GAAGAAAAAT GGTACTCGGA AGAATTAAAG AAGGAATTAG AAAAATATCA AGGGCTGCAG 
CAGCAAGAGG CCAAACCTGA TGGGTTGGTG ACTGATTCAT CAGCAGAACT ACAGTCTTTG 
GAACAGCAAT TAGAAGAAGC CCAAACAGAA AATTTTAATA TTAAGCAAAT GAAAGACTTA 
TTTGAACAGA AAGCAGCCCA ACTTGCTACT GAAATTGCAG ATATAAAGTC AAAGTATGAT 
GAAGAAAGGA GTCTTCGAGA AGCTGCTGAA CAAAAAGTGA CACGTCTGAC AGAAGAATTA 
AACAAAGAGG CAACTGTAAT TCAAGATCTG AAGACGGAAC TGCTTCAGAG ACCTGGTATA 
GAAGATGTTG CCGTGCTAAA GAAAGAACTG GTCCAAGTTC AAACACTAAT GGATAACATG 
ACCTTGGAAC GTGAGCGAGA ATCTGAAAAA CTCAAAGATG AATGCAAAAA ATTGCAGTCA 
CAATATGCTA GCTCAGAGGC CACAATAAGC CAGCTAAGGA GTGAACTTGC CAAAGGCCCC 
CAGGAAGTTG CTGTATATGT ACAGGAACTA CAAAAACTGA AAAGTTCAGT TAATGAATTA 
ACACAAAAAA ATCAGACCTT GACAGAAAAC TTGCTGAAAA AAGAACAAGA CTATACTAAG 
TTAGAGGAGA AACATAATGA AGAATCTGTG AGTAAAAAGA ATATTCAGGC AACCCTTCAT 
CAAAAAGACC TAGATTGTCA ACAGCTTCAG TCAAGATTGT CTGCATCTGA AACCTCACTG 
CATAGAATAC ATGTAGAACT AAGTGAAAAA GGAGAAGCTA CTCAAAAGCT CAAAGAAGAA 
TTATCTGAGG TAGAGACCAA GTACCAGCAT CTAAAGGCGG AGTTTAAGCA GCTACAACAA 
CAGAGAGAAG AAAAGGAGCA GCATGGGTTA CAACTCCAAA GTGAAATTAA TCAATTACAT 
AGCAAACTTC TGGAGACAGA GCGCCAACTA GGGGAAGCTC ATGGTAGGCT GAAGGAACAG 
AGACAGCTTT CAAGTGAAAA GTTGATGGAT AAAGAACAAC AAGTGGCTGA TTTACAACTC 
AAACTTTCTC GGTTAGAAGA GCAGTTGAAG GAAAAAGTTA CAAATTCTAC AGAATTGCAG 
CATCAATTAG ATAAAACAAA GCAACAGCAT CAAGAACAAC AGGCTCTTCA GCAAAGCACC 
ACGGCAAAAC TTCGAGAAGC TCAGAATGAT TTGGAACAAG TTCTACGTCA AATTGGCGAT 
AAGGACCAAA AGATCCAGAA CCTTGAAGCT TTATTACAGA AGAGTAAAGA AAATATTTCA 
TTACTAGAAA AAGAAAGAGA AGATCTTTAT GCAAAAATTC AGGCTGGTGA AGGAGAGACT 
GCTGTTCTTA ACCAGTTACA AGAAAAAAAC CATACACTAC AGGAGCAAGT AACTCAACTA 
ACAGAGAAGC TGAAGAATCA GTCAGAAAGT CATAAACAAG CCCAGGAGAA TTTGCATGAC 
CAGGTACAAG AGCAGAAGGC ACATCTTAGA GCTGCACAAG ACCGTGTCCT TTCCCTAGAA 
ACTAGTGTCA ATGAATTAAA TAGTCAATTA AATGAAAGCA AGGAGAAGGT CTCCCAGCTT 
GACATACAGA TTAAAGCCAA AACCGAACTA TTACTATCAG CAGAAGCAGC AAAAACTGCT 
CAAAGAGCTG ATCTTCAGAA TCATTTGGAC ACAGCTCAAA ATGCATTACA AGATAAACAG 
CAGGAGTTAA ATAAGATTAC TACTCAGTTG GATCAGGTCA CTGCAAAGTT ACAAGACAAG 
CAAGAACATT GCAGTCAGCT GGAAAGTCAT CTTAAAGAAT ATAAAGAGAA ATACCTCTCT 
TTAGAACAGA AAACCGAAGA GCTAGAAGGT CAAATTAAGA AACTAGAAGC TGATAGTCTT 
GAAGTTAAAG CAAGCAAGGA GCAGGCTTTG CAAGATCTAC AACAGCAAAG ACAGCTGAAC 
ACAGATTTAG AGCTCAGAGC CACAGAATTG AGTAAACAAC TTGAAATGGA GAAGGAAATA 
GTATCCAGTA CAAGATTGGA TCTACAGAAA AAATCTGAAG CCCTTGAAAG TATCAAGCAA 
AAGCTTACCA AGCAAGAGGA AGAAAAAAAA ATCCTGAAAC AAGATTTTGA AACTTTAAGT 
CAAGAAACAA AGATTCAGCA TGAGGAATTG AATAACAGAA TTCAAACAAC AGTAACAGAA 
CTACAAAAAG TGAAAATGGA GAAAGAAGCT TTAATGACAG AGCTTTCTAC AGTAAAGGAC 
AAACTATCAA AAGTTTCTGA TTCTTTGAAA AACTCTAAAA GTGAATTTGA AAAGGAGAAT 
CAGAAAGGAA AAGCCGCTAT ATTAGACTTG GAAAAAACTT GCAAAGAATT AAAGCATCAA 
CTTCAAGTGC AGATGGAAAA CACACTTAAG GAACAGAAGG AACTGAAAAA GTCACTTGAA 
AAAGAGAAGG AGGCTTCTCA TCAGTTGAAA TTGGAACTCA ATTCAATGCA GGAACAACTT 
ATACAGGCCC AGAATACTTT AAAACAAAAT GAAAAAGAAG AGCAACAACT TCAGGGGAAC 
ATAAATGAGC TAAAGCAATC AAGTGAACAG AAGAAAAAAC AAATTGAAGC ACTCCAAGGA 
GAGCTTAAAA TTGCTGTTTT ACAGAAGACA GAGCTTGAGA ATAAACTACA GCAGCAGTTA 
ACACAGGCAG CCCAGGAACT TGCAGCAGAG AAAGAGAAAA TATCAGTATT ACAAAACAAC 
TATGAAAAAA GTCAGGAAAC TTTCAAACAG CTTCAATCTG ATTTCTATGG GAGGGAATCT 
GAACTTCTAG CCACCAGGCA AGATCTTAAG TCTGTAGAAG AGAAGCTTTC TCTAGCACAG 
GAGGACTTGA TTTCAAACAG AAATCAAATT GGAAATCAAA ATAAATTGAT TCAAGAACTG 
AAGACTGCCA AGGCTACATT GGAGCAGGAT TCAGCAAAGA AAGAACAGCA ATTGCAGGAG 
CGATGTAAAG CACTACAAGA CATTCAGAAA GAAAAGTCAC TGAAAGAAAA AGAACTGGTA 
AATGAGAAGT CTAAATTGGC AGAGATAGAA GAAATTAAAT GTAGACAAGA AAAAGAAATC 
ACTAAACTAA ACGAAGAACT CAAGTCCCAC AAACTAGAAA GCATAAAGGA GATAACAAAT 
CTTAAAGATG CTAAACAGCT TCTAATTCAG CAGAAATTAG AACTTCAAGG AAAAGCGGAC 
TCCCTGAAGG CAGCTGTTGA ACAGGAGAAG AGAAATCAGC AGATACTAAA AGACCAGGTG 
AAAAAGGAAG AAGAGGAGCT GAAGAAAGAA TTTATTGAGA AAGAAGCTAA GTTGCATTCC 
GAAATAAAAG AAAAGGAAGT AGGAATGAAG AAGCATGAAG AAAATGAGGC TAAACTTACC 
ATGCAGATTA CAGCATTAAA TGAAAACTTA GGCACTGTGA AGAAGGAGTG GCAATCTAGT 
CAACGGAGAG TTAGTGAGCT TGAGAAACAA ACGGATGACT TACGGGGTGA AATTGCAGTA 
TTAGAAGCAA CGGTTCAGAA TAATCAAGAT GAAAGGAGAG CACTACTGGA AAGATGTCTT 
AAAGGAGAAG GTGAAATAGA AAAGCTTCAA ACCAAAGTAT TAGAATTGCA AAGAAAGCTG 
GATAATACAA CTGCAGCAGT GCAGGAGCTG GGCAGAGAAA ACCAATCACT TCAGATCAAA 
CATACACAAG CGTTGAATAG AAAGTGGGCC GAAGACAATG AAGTACAAAA CTGTATGGCC 
TGTGGGAAAG GCTTTTCAGT AACAGTGAGA CGGCATCACT GCCGACAGTG TGGAAATATC 
TTCTGTGCTG AATGTTCAGC CAAAAATGCC TTAACTCCTT CCTCCAAGAA GCCTGTTCGT 
GTCTGTGATG CATGTTTCAA TGACTTGCAA GGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003557.3
CDS	230..4465
Translation	MLRRILQRTPGRVGSQGSDLDSSATPINTVDVNNESSSEGFICPQCMKSLGSADELFKHYEAVHDAGNDSGHGGESNLALKRDDVTLLRQEVQDLQASLKEEKWYSEELKKELEKYQGLQQQEAKPDGLVTDSSAELQSLEQQLEEAQTENFNIKQMKDLFEQKAAQLATEIADIKSKYDEERSLREAAEQKVTRLTEELNKEATVIQDLKTELLQRPGIEDVAVLKKELVQVQTLMDNMTLERERESEKLKDECKKLQSQYASSEATISQLRSELAKGPQEVAVYVQELQKLKSSVNELTQKNQTLTENLLKKEQDYTKLEEKHNEESVSKKNIQATLHQKDLDCQQLQSRLSASETSLHRIHVELSEKGEATQKLKEELSEVETKYQHLKAEFKQLQQQREEKEQHGLQLQSEINQLHSKLLETERQLGEAHGRLKEQRQLSSEKLMDKEQQVADLQLKLSRLEEQLKEKVTNSTELQHQLDKTKQQHQEQQALQQSTTAKLREAQNDLEQVLRQIGDKDQKIQNLEALLQKSKENISLLEKEREDLYAKIQAGEGETAVLNQLQEKNHTLQEQVTQLTEKLKNQSESHKQAQENLHDQVQEQKAHLRAAQDRVLSLETSVNELNSQLNESKEKVSQLDIQIKAKTELLLSAEAAKTAQRADLQNHLDTAQNALQDKQQELNKITTQLDQVTAKLQDKQEHCSQLESHLKEYKEKYLSLEQKTEELEGQIKKLEADSLEVKASKEQALQDLQQQRQLNTDLELRATELSKQLEMEKEIVSSTRLDLQKKSEALESIKQKLTKQEEEKKILKQDFETLSQETKIQHEELNNRIQTTVTELQKVKMEKEALMTELSTVKDKLSKVSDSLKNSKSEFEKENQKGKAAILDLEKTCKELKHQLQVQMENTLKEQKELKKSLEKEKEASHQLKLELNSMQEQLIQAQNTLKQNEKEEQQLQGNINELKQSSEQKKKQIEALQGELKIAVLQKTELENKLQQQLTQAAQELAAEKEKISVLQNNYEKSQETFKQLQSDFYGRESELLATRQDLKSVEEKLSLAQEDLISNRNQIGNQNKLIQELKTAKATLEQDSAKKEQQLQERCKALQDIQKEKSLKEKELVNEKSKLAEIEEIKCRQEKEITKLNEELKSHKLESIKEITNLKDAKQLLIQQKLELQGKADSLKAAVEQEKRNQQILKDQVKKEEEELKKEFIEKEAKLHSEIKEKEVGMKKHEENEAKLTMQITALNENLGTVKKEWQSSQRRVSELEKQTDDLRGEIAVLEATVQNNQDERRALLERCLKGEGEIEKLQTKVLELQRKLDNTTAAVQELGRENQSLQIKHTQALNRKWAEDNEVQNCMACGKGFSVTVRRHHCRQCGNIFCAECSAKNALTPSSKKPVRVCDACFNDLQG

Target ORF information:

RefSeq Version	NM_003566.4
Organism	Homo sapiens(human)
Definition	Homo sapiens early endosome antigen 1 (EEA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003566.4

ORF Insert Sequence:

ATGTTAAGGA GGATTTTACA GAGGACTCCT GGGAGAGTTG GCTCTCAAGG TTCTGATTTA 
GATTCATCAG CAACTCCTAT AAACACAGTG GACGTCAATA ATGAAAGCTC TTCAGAGGGT 
TTCATATGTC CCCAGTGTAT GAAATCTCTT GGATCTGCTG ATGAACTTTT CAAACATTAT 
GAAGCTGTTC ATGATGCTGG TAATGACTCA GGTCATGGAG GAGAGTCTAA TCTTGCTTTG 
AAGCGAGATG ATGTAACACT GCTCAGACAA GAGGTCCAAG ACCTACAGGC TTCACTTAAG 
GAAGAAAAAT GGTACTCGGA AGAATTAAAG AAGGAATTAG AAAAATATCA AGGGCTGCAG 
CAGCAAGAGG CCAAACCTGA TGGGTTGGTG ACTGATTCAT CAGCAGAACT ACAGTCTTTG 
GAACAGCAAT TAGAAGAAGC CCAAACAGAA AATTTTAATA TTAAGCAAAT GAAAGACTTA 
TTTGAACAGA AAGCAGCCCA ACTTGCTACT GAAATTGCAG ATATAAAGTC AAAGTATGAT 
GAAGAAAGGA GTCTTCGAGA AGCTGCTGAA CAAAAAGTGA CACGTCTGAC AGAAGAATTA 
AACAAAGAGG CAACTGTAAT TCAAGATCTG AAGACGGAAC TGCTTCAGAG ACCTGGTATA 
GAAGATGTTG CCGTGCTAAA GAAAGAACTG GTCCAAGTTC AAACACTAAT GGATAACATG 
ACCTTGGAAC GTGAGCGAGA ATCTGAAAAA CTCAAAGATG AATGCAAAAA ATTGCAGTCA 
CAATATGCTA GCTCAGAGGC CACAATAAGC CAGCTAAGGA GTGAACTTGC CAAAGGCCCC 
CAGGAAGTTG CTGTATATGT ACAGGAACTA CAAAAACTGA AAAGTTCAGT TAATGAATTA 
ACACAAAAAA ATCAGACCTT GACAGAAAAC TTGCTGAAAA AAGAACAAGA CTATACTAAG 
TTAGAGGAGA AACATAATGA AGAATCTGTG AGTAAAAAGA ATATTCAGGC AACCCTTCAT 
CAAAAAGACC TAGATTGTCA ACAGCTTCAG TCAAGATTGT CTGCATCTGA AACCTCACTG 
CATAGAATAC ATGTAGAACT AAGTGAAAAA GGAGAAGCTA CTCAAAAGCT CAAAGAAGAA 
TTATCTGAGG TAGAGACCAA GTACCAGCAT CTAAAGGCGG AGTTTAAGCA GCTACAACAA 
CAGAGAGAAG AAAAGGAGCA GCATGGGTTA CAACTCCAAA GTGAAATTAA TCAATTACAT 
AGCAAACTTC TGGAGACAGA GCGCCAACTA GGGGAAGCTC ATGGTAGGCT GAAGGAACAG 
AGACAGCTTT CAAGTGAAAA GTTGATGGAT AAAGAACAAC AAGTGGCTGA TTTACAACTC 
AAACTTTCTC GGTTAGAAGA GCAGTTGAAG GAAAAAGTTA CAAATTCTAC AGAATTGCAG 
CATCAATTAG ATAAAACAAA GCAACAGCAT CAAGAACAAC AGGCTCTTCA GCAAAGCACC 
ACGGCAAAAC TTCGAGAAGC TCAGAATGAT TTGGAACAAG TTCTACGTCA AATTGGCGAT 
AAGGACCAAA AGATCCAGAA CCTTGAAGCT TTATTACAGA AGAGTAAAGA AAATATTTCA 
TTACTAGAAA AAGAAAGAGA AGATCTTTAT GCAAAAATTC AGGCTGGTGA AGGAGAGACT 
GCTGTTCTTA ACCAGTTACA AGAAAAAAAC CATACACTAC AGGAGCAAGT AACTCAACTA 
ACAGAGAAGC TGAAGAATCA GTCAGAAAGT CATAAACAAG CCCAGGAGAA TTTGCATGAC 
CAGGTACAAG AGCAGAAGGC ACATCTTAGA GCTGCACAAG ACCGTGTCCT TTCCCTAGAA 
ACTAGTGTCA ATGAATTAAA TAGTCAATTA AATGAAAGCA AGGAGAAGGT CTCCCAGCTT 
GACATACAGA TTAAAGCCAA AACCGAACTA TTACTATCAG CAGAAGCAGC AAAAACTGCT 
CAAAGAGCTG ATCTTCAGAA TCATTTGGAC ACAGCTCAAA ATGCATTACA AGATAAACAG 
CAGGAGTTAA ATAAGATTAC TACTCAGTTG GATCAGGTCA CTGCAAAGTT ACAAGACAAG 
CAAGAACATT GCAGTCAGCT GGAAAGTCAT CTTAAAGAAT ATAAAGAGAA ATACCTCTCT 
TTAGAACAGA AAACCGAAGA GCTAGAAGGT CAAATTAAGA AACTAGAAGC TGATAGTCTT 
GAAGTTAAAG CAAGCAAGGA GCAGGCTTTG CAAGATCTAC AACAGCAAAG ACAGCTGAAC 
ACAGATTTAG AGCTCAGAGC CACAGAATTG AGTAAACAAC TTGAAATGGA GAAGGAAATA 
GTATCCAGTA CAAGATTGGA TCTACAGAAA AAATCTGAAG CCCTTGAAAG TATCAAGCAA 
AAGCTTACCA AGCAAGAGGA AGAAAAAAAA ATCCTGAAAC AAGATTTTGA AACTTTAAGT 
CAAGAAACAA AGATTCAGCA TGAGGAATTG AATAACAGAA TTCAAACAAC AGTAACAGAA 
CTACAAAAAG TGAAAATGGA GAAAGAAGCT TTAATGACAG AGCTTTCTAC AGTAAAGGAC 
AAACTATCAA AAGTTTCTGA TTCTTTGAAA AACTCTAAAA GTGAATTTGA AAAGGAGAAT 
CAGAAAGGAA AAGCCGCTAT ATTAGACTTG GAAAAAACTT GCAAAGAATT AAAGCATCAA 
CTTCAAGTGC AGATGGAAAA CACACTTAAG GAACAGAAGG AACTGAAAAA GTCACTTGAA 
AAAGAGAAGG AGGCTTCTCA TCAGTTGAAA TTGGAACTCA ATTCAATGCA GGAACAACTT 
ATACAGGCCC AGAATACTTT AAAACAAAAT GAAAAAGAAG AGCAACAACT TCAGGGGAAC 
ATAAATGAGC TAAAGCAATC AAGTGAACAG AAGAAAAAAC AAATTGAAGC ACTCCAAGGA 
GAGCTTAAAA TTGCTGTTTT ACAGAAGACA GAGCTTGAGA ATAAACTACA GCAGCAGTTA 
ACACAGGCAG CCCAGGAACT TGCAGCAGAG AAAGAGAAAA TATCAGTATT ACAAAACAAC 
TATGAAAAAA GTCAGGAAAC TTTCAAACAG CTTCAATCTG ATTTCTATGG GAGGGAATCT 
GAACTTCTAG CCACCAGGCA AGATCTTAAG TCTGTAGAAG AGAAGCTTTC TCTAGCACAG 
GAGGACTTGA TTTCAAACAG AAATCAAATT GGAAATCAAA ATAAATTGAT TCAAGAACTG 
AAGACTGCCA AGGCTACATT GGAGCAGGAT TCAGCAAAGA AAGAACAGCA ATTGCAGGAG 
CGATGTAAAG CACTACAAGA CATTCAGAAA GAAAAGTCAC TGAAAGAAAA AGAACTGGTA 
AATGAGAAGT CTAAATTGGC AGAGATAGAA GAAATTAAAT GTAGACAAGA AAAAGAAATC 
ACTAAACTAA ACGAAGAACT CAAGTCCCAC AAACTAGAAA GCATAAAGGA GATAACAAAT 
CTTAAAGATG CTAAACAGCT TCTAATTCAG CAGAAATTAG AACTTCAAGG AAAAGCGGAC 
TCCCTGAAGG CAGCTGTTGA ACAGGAGAAG AGAAATCAGC AGATACTAAA AGACCAGGTG 
AAAAAGGAAG AAGAGGAGCT GAAGAAAGAA TTTATTGAGA AAGAAGCTAA GTTGCATTCC 
GAAATAAAAG AAAAGGAAGT AGGAATGAAG AAGCATGAAG AAAATGAGGC TAAACTTACC 
ATGCAGATTA CAGCATTAAA TGAAAACTTA GGCACTGTGA AGAAGGAGTG GCAATCTAGT 
CAACGGAGAG TTAGTGAGCT TGAGAAACAA ACGGATGACT TACGGGGTGA AATTGCAGTA 
TTAGAAGCAA CGGTTCAGAA TAATCAAGAT GAAAGGAGAG CACTACTGGA AAGATGTCTT 
AAAGGAGAAG GTGAAATAGA AAAGCTTCAA ACCAAAGTAT TAGAATTGCA AAGAAAGCTG 
GATAATACAA CTGCAGCAGT GCAGGAGCTG GGCAGAGAAA ACCAATCACT TCAGATCAAA 
CATACACAAG CGTTGAATAG AAAGTGGGCC GAAGACAATG AAGTACAAAA CTGTATGGCC 
TGTGGGAAAG GCTTTTCAGT AACAGTGAGA CGGCATCACT GCCGACAGTG TGGAAATATC 
TTCTGTGCTG AATGTTCAGC CAAAAATGCC TTAACTCCTT CCTCCAAGAA GCCTGTTCGT 
GTCTGTGATG CATGTTTCAA TGACTTGCAA GGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EEA1 ( XM_017020018.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016875507.1 Homo sapiens early endosome antigen 1 (EEA1), transcript variant X2, mRNA.

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CloneID	OHu84196
Clone ID Related Accession (Same CDS sequence)	XM_017020018.1
Accession Version	XM_017020018.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4254bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	early endosome antigen 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCACAG TGGAAACCAG CAGCCTGTCA GCCACTGGAA AGACTCCTGG GAGAGTTGGC 
TCTCAAGGTT CTGATTTAGA TTCATCAGCA ACTCCTATAA ACACAGTGGA CGTCAATAAT 
GAAAGCTCTT CAGAGGGTTT CATATGTCCC CAGTGTATGA AATCTCTTGG ATCTGCTGAT 
GAACTTTTCA AACATTATGA AGCTGTTCAT GATGCTGGTA ATGACTCAGG TCATGGAGGA 
GAGTCTAATC TTGCTTTGAA GCGAGATGAT GTAACACTGC TCAGACAAGA GGTCCAAGAC 
CTACAGGCTT CACTTAAGGA AGAAAAATGG TACTCGGAAG AATTAAAGAA GGAATTAGAA 
AAATATCAAG GGCTGCAGCA GCAAGAGGCC AAACCTGATG GGTTGGTGAC TGATTCATCA 
GCAGAACTAC AGTCTTTGGA ACAGCAATTA GAAGAAGCCC AAACAGAAAA TTTTAATATT 
AAGCAAATGA AAGACTTATT TGAACAGAAA GCAGCCCAAC TTGCTACTGA AATTGCAGAT 
ATAAAGTCAA AGTATGATGA AGAAAGGAGT CTTCGAGAAG CTGCTGAACA AAAAGTGACA 
CGTCTGACAG AAGAATTAAA CAAAGAGGCA ACTGTAATTC AAGATCTGAA GACGGAACTG 
CTTCAGAGAC CTGGTATAGA AGATGTTGCC GTGCTAAAGA AAGAACTGGT CCAAGTTCAA 
ACACTAATGG ATAACATGAC CTTGGAACGT GAGCGAGAAT CTGAAAAACT CAAAGATGAA 
TGCAAAAAAT TGCAGTCACA ATATGCTAGC TCAGAGGCCA CAATAAGCCA GCTAAGGAGT 
GAACTTGCCA AAGGCCCCCA GGAAGTTGCT GTATATGTAC AGGAACTACA AAAACTGAAA 
AGTTCAGTTA ATGAATTAAC ACAAAAAAAT CAGACCTTGA CAGAAAACTT GCTGAAAAAA 
GAACAAGACT ATACTAAGTT AGAGGAGAAA CATAATGAAG AATCTGTGAG TAAAAAGAAT 
ATTCAGGCAA CCCTTCATCA AAAAGACCTA GATTGTCAAC AGCTTCAGTC AAGATTGTCT 
GCATCTGAAA CCTCACTGCA TAGAATACAT GTAGAACTAA GTGAAAAAGG AGAAGCTACT 
CAAAAGCTCA AAGAAGAATT ATCTGAGGTA GAGACCAAGT ACCAGCATCT AAAGGCGGAG 
TTTAAGCAGC TACAACAACA GAGAGAAGAA AAGGAGCAGC ATGGGTTACA ACTCCAAAGT 
GAAATTAATC AATTACATAG CAAACTTCTG GAGACAGAGC GCCAACTAGG GGAAGCTCAT 
GGTAGGCTGA AGGAACAGAG ACAGCTTTCA AGTGAAAAGT TGATGGATAA AGAACAACAA 
GTGGCTGATT TACAACTCAA ACTTTCTCGG TTAGAAGAGC AGTTGAAGGA AAAAGTTACA 
AATTCTACAG AATTGCAGCA TCAATTAGAT AAAACAAAGC AACAGCATCA AGAACAACAG 
GCTCTTCAGC AAAGCACCAC GGCAAAACTT CGAGAAGCTC AGAATGATTT GGAACAAGTT 
CTACGTCAAA TTGGCGATAA GGACCAAAAG ATCCAGAACC TTGAAGCTTT ATTACAGAAG 
AGTAAAGAAA ATATTTCATT ACTAGAAAAA GAAAGAGAAG ATCTTTATGC AAAAATTCAG 
GCTGGTGAAG GAGAGACTGC TGTTCTTAAC CAGTTACAAG AAAAAAACCA TACACTACAG 
GAGCAAGTAA CTCAACTAAC AGAGAAGCTG AAGAATCAGT CAGAAAGTCA TAAACAAGCC 
CAGGAGAATT TGCATGACCA GGTACAAGAG CAGAAGGCAC ATCTTAGAGC TGCACAAGAC 
CGTGTCCTTT CCCTAGAAAC TAGTGTCAAT GAATTAAATA GTCAATTAAA TGAAAGCAAG 
GAGAAGGTCT CCCAGCTTGA CATACAGATT AAAGCCAAAA CCGAACTATT ACTATCAGCA 
GAAGCAGCAA AAACTGCTCA AAGAGCTGAT CTTCAGAATC ATTTGGACAC AGCTCAAAAT 
GCATTACAAG ATAAACAGCA GGAGTTAAAT AAGATTACTA CTCAGTTGGA TCAGGTCACT 
GCAAAGTTAC AAGACAAGCA AGAACATTGC AGTCAGCTGG AAAGTCATCT TAAAGAATAT 
AAAGAGAAAT ACCTCTCTTT AGAACAGAAA ACCGAAGAGC TAGAAGGTCA AATTAAGAAA 
CTAGAAGCTG ATAGTCTTGA AGTTAAAGCA AGCAAGGAGC AGGCTTTGCA AGATCTACAA 
CAGCAAAGAC AGCTGAACAC AGATTTAGAG CTCAGAGCCA CAGAATTGAG TAAACAACTT 
GAAATGGAGA AGGAAATAGT ATCCAGTACA AGATTGGATC TACAGAAAAA ATCTGAAGCC 
CTTGAAAGTA TCAAGCAAAA GCTTACCAAG CAAGAGGAAG AAAAAAAAAT CCTGAAACAA 
GATTTTGAAA CTTTAAGTCA AGAAACAAAG ATTCAGCATG AGGAATTGAA TAACAGAATT 
CAAACAACAG TAACAGAACT ACAAAAAGTG AAAATGGAGA AAGAAGCTTT AATGACAGAG 
CTTTCTACAG TAAAGGACAA ACTATCAAAA GTTTCTGATT CTTTGAAAAA CTCTAAAAGT 
GAATTTGAAA AGGAGAATCA GAAAGGAAAA GCCGCTATAT TAGACTTGGA AAAAACTTGC 
AAAGAATTAA AGCATCAACT TCAAGTGCAG ATGGAAAACA CACTTAAGGA ACAGAAGGAA 
CTGAAAAAGT CACTTGAAAA AGAGAAGGAG GCTTCTCATC AGTTGAAATT GGAACTCAAT 
TCAATGCAGG AACAACTTAT ACAGGCCCAG AATACTTTAA AACAAAATGA AAAAGAAGAG 
CAACAACTTC AGGGGAACAT AAATGAGCTA AAGCAATCAA GTGAACAGAA GAAAAAACAA 
ATTGAAGCAC TCCAAGGAGA GCTTAAAATT GCTGTTTTAC AGAAGACAGA GCTTGAGAAT 
AAACTACAGC AGCAGTTAAC ACAGGCAGCC CAGGAACTTG CAGCAGAGAA AGAGAAAATA 
TCAGTATTAC AAAACAACTA TGAAAAAAGT CAGGAAACTT TCAAACAGCT TCAATCTGAT 
TTCTATGGGA GGGAATCTGA ACTTCTAGCC ACCAGGCAAG ATCTTAAGTC TGTAGAAGAG 
AAGCTTTCTC TAGCACAGGA GGACTTGATT TCAAACAGAA ATCAAATTGG AAATCAAAAT 
AAATTGATTC AAGAACTGAA GACTGCCAAG GCTACATTGG AGCAGGATTC AGCAAAGAAA 
GAACAGCAAT TGCAGGAGCG ATGTAAAGCA CTACAAGACA TTCAGAAAGA AAAGTCACTG 
AAAGAAAAAG AACTGGTAAA TGAGAAGTCT AAATTGGCAG AGATAGAAGA AATTAAATGT 
AGACAAGAAA AAGAAATCAC TAAACTAAAC GAAGAACTCA AGTCCCACAA ACTAGAAAGC 
ATAAAGGAGA TAACAAATCT TAAAGATGCT AAACAGCTTC TAATTCAGCA GAAATTAGAA 
CTTCAAGGAA AAGCGGACTC CCTGAAGGCA GCTGTTGAAC AGGAGAAGAG AAATCAGCAG 
ATACTAAAAG ACCAGGTGAA AAAGGAAGAA GAGGAGCTGA AGAAAGAATT TATTGAGAAA 
GAAGCTAAGT TGCATTCCGA AATAAAAGAA AAGGAAGTAG GAATGAAGAA GCATGAAGAA 
AATGAGGCTA AACTTACCAT GCAGATTACA GCATTAAATG AAAACTTAGG CACTGTGAAG 
AAGGAGTGGC AATCTAGTCA ACGGAGAGTT AGTGAGCTTG AGAAACAAAC GGATGACTTA 
CGGGGTGAAA TTGCAGTATT AGAAGCAACG GTTCAGAATA ATCAAGATGA AAGGAGAGCA 
CTACTGGAAA GATGTCTTAA AGGAGAAGGT GAAATAGAAA AGCTTCAAAC CAAAGTATTA 
GAATTGCAAA GAAAGCTGGA TAATACAACT GCAGCAGTGC AGGAGCTGGG CAGAGAAAAC 
CAATCACTTC AGATCAAACA TACACAAGCG TTGAATAGAA AGTGGGCCGA AGACAATGAA 
GTACAAAACT GTATGGCCTG TGGGAAAGGC TTTTCAGTAA CAGTGAGACG GCATCACTGC 
CGACAGTGTG GAAATATCTT CTGTGCTGAA TGTTCAGCCA AAAATGCCTT AACTCCTTCC 
TCCAAGAAGC CTGTTCGTGT CTGTGATGCA TGTTTCAATG ACTTGCAAGG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016875507.1
CDS	16..4269
Translation	MITVETSSLSATGKTPGRVGSQGSDLDSSATPINTVDVNNESSSEGFICPQCMKSLGSADELFKHYEAVHDAGNDSGHGGESNLALKRDDVTLLRQEVQDLQASLKEEKWYSEELKKELEKYQGLQQQEAKPDGLVTDSSAELQSLEQQLEEAQTENFNIKQMKDLFEQKAAQLATEIADIKSKYDEERSLREAAEQKVTRLTEELNKEATVIQDLKTELLQRPGIEDVAVLKKELVQVQTLMDNMTLERERESEKLKDECKKLQSQYASSEATISQLRSELAKGPQEVAVYVQELQKLKSSVNELTQKNQTLTENLLKKEQDYTKLEEKHNEESVSKKNIQATLHQKDLDCQQLQSRLSASETSLHRIHVELSEKGEATQKLKEELSEVETKYQHLKAEFKQLQQQREEKEQHGLQLQSEINQLHSKLLETERQLGEAHGRLKEQRQLSSEKLMDKEQQVADLQLKLSRLEEQLKEKVTNSTELQHQLDKTKQQHQEQQALQQSTTAKLREAQNDLEQVLRQIGDKDQKIQNLEALLQKSKENISLLEKEREDLYAKIQAGEGETAVLNQLQEKNHTLQEQVTQLTEKLKNQSESHKQAQENLHDQVQEQKAHLRAAQDRVLSLETSVNELNSQLNESKEKVSQLDIQIKAKTELLLSAEAAKTAQRADLQNHLDTAQNALQDKQQELNKITTQLDQVTAKLQDKQEHCSQLESHLKEYKEKYLSLEQKTEELEGQIKKLEADSLEVKASKEQALQDLQQQRQLNTDLELRATELSKQLEMEKEIVSSTRLDLQKKSEALESIKQKLTKQEEEKKILKQDFETLSQETKIQHEELNNRIQTTVTELQKVKMEKEALMTELSTVKDKLSKVSDSLKNSKSEFEKENQKGKAAILDLEKTCKELKHQLQVQMENTLKEQKELKKSLEKEKEASHQLKLELNSMQEQLIQAQNTLKQNEKEEQQLQGNINELKQSSEQKKKQIEALQGELKIAVLQKTELENKLQQQLTQAAQELAAEKEKISVLQNNYEKSQETFKQLQSDFYGRESELLATRQDLKSVEEKLSLAQEDLISNRNQIGNQNKLIQELKTAKATLEQDSAKKEQQLQERCKALQDIQKEKSLKEKELVNEKSKLAEIEEIKCRQEKEITKLNEELKSHKLESIKEITNLKDAKQLLIQQKLELQGKADSLKAAVEQEKRNQQILKDQVKKEEEELKKEFIEKEAKLHSEIKEKEVGMKKHEENEAKLTMQITALNENLGTVKKEWQSSQRRVSELEKQTDDLRGEIAVLEATVQNNQDERRALLERCLKGEGEIEKLQTKVLELQRKLDNTTAAVQELGRENQSLQIKHTQALNRKWAEDNEVQNCMACGKGFSVTVRRHHCRQCGNIFCAECSAKNALTPSSKKPVRVCDACFNDLQG

Target ORF information:

RefSeq Version	XM_017020018.1
Organism	Homo sapiens(human)
Definition	Homo sapiens early endosome antigen 1 (EEA1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017020018.1

ORF Insert Sequence:

ATGATCACAG TGGAAACCAG CAGCCTGTCA GCCACTGGAA AGACTCCTGG GAGAGTTGGC 
TCTCAAGGTT CTGATTTAGA TTCATCAGCA ACTCCTATAA ACACAGTGGA CGTCAATAAT 
GAAAGCTCTT CAGAGGGTTT CATATGTCCC CAGTGTATGA AATCTCTTGG ATCTGCTGAT 
GAACTTTTCA AACATTATGA AGCTGTTCAT GATGCTGGTA ATGACTCAGG TCATGGAGGA 
GAGTCTAATC TTGCTTTGAA GCGAGATGAT GTAACACTGC TCAGACAAGA GGTCCAAGAC 
CTACAGGCTT CACTTAAGGA AGAAAAATGG TACTCGGAAG AATTAAAGAA GGAATTAGAA 
AAATATCAAG GGCTGCAGCA GCAAGAGGCC AAACCTGATG GGTTGGTGAC TGATTCATCA 
GCAGAACTAC AGTCTTTGGA ACAGCAATTA GAAGAAGCCC AAACAGAAAA TTTTAATATT 
AAGCAAATGA AAGACTTATT TGAACAGAAA GCAGCCCAAC TTGCTACTGA AATTGCAGAT 
ATAAAGTCAA AGTATGATGA AGAAAGGAGT CTTCGAGAAG CTGCTGAACA AAAAGTGACA 
CGTCTGACAG AAGAATTAAA CAAAGAGGCA ACTGTAATTC AAGATCTGAA GACGGAACTG 
CTTCAGAGAC CTGGTATAGA AGATGTTGCC GTGCTAAAGA AAGAACTGGT CCAAGTTCAA 
ACACTAATGG ATAACATGAC CTTGGAACGT GAGCGAGAAT CTGAAAAACT CAAAGATGAA 
TGCAAAAAAT TGCAGTCACA ATATGCTAGC TCAGAGGCCA CAATAAGCCA GCTAAGGAGT 
GAACTTGCCA AAGGCCCCCA GGAAGTTGCT GTATATGTAC AGGAACTACA AAAACTGAAA 
AGTTCAGTTA ATGAATTAAC ACAAAAAAAT CAGACCTTGA CAGAAAACTT GCTGAAAAAA 
GAACAAGACT ATACTAAGTT AGAGGAGAAA CATAATGAAG AATCTGTGAG TAAAAAGAAT 
ATTCAGGCAA CCCTTCATCA AAAAGACCTA GATTGTCAAC AGCTTCAGTC AAGATTGTCT 
GCATCTGAAA CCTCACTGCA TAGAATACAT GTAGAACTAA GTGAAAAAGG AGAAGCTACT 
CAAAAGCTCA AAGAAGAATT ATCTGAGGTA GAGACCAAGT ACCAGCATCT AAAGGCGGAG 
TTTAAGCAGC TACAACAACA GAGAGAAGAA AAGGAGCAGC ATGGGTTACA ACTCCAAAGT 
GAAATTAATC AATTACATAG CAAACTTCTG GAGACAGAGC GCCAACTAGG GGAAGCTCAT 
GGTAGGCTGA AGGAACAGAG ACAGCTTTCA AGTGAAAAGT TGATGGATAA AGAACAACAA 
GTGGCTGATT TACAACTCAA ACTTTCTCGG TTAGAAGAGC AGTTGAAGGA AAAAGTTACA 
AATTCTACAG AATTGCAGCA TCAATTAGAT AAAACAAAGC AACAGCATCA AGAACAACAG 
GCTCTTCAGC AAAGCACCAC GGCAAAACTT CGAGAAGCTC AGAATGATTT GGAACAAGTT 
CTACGTCAAA TTGGCGATAA GGACCAAAAG ATCCAGAACC TTGAAGCTTT ATTACAGAAG 
AGTAAAGAAA ATATTTCATT ACTAGAAAAA GAAAGAGAAG ATCTTTATGC AAAAATTCAG 
GCTGGTGAAG GAGAGACTGC TGTTCTTAAC CAGTTACAAG AAAAAAACCA TACACTACAG 
GAGCAAGTAA CTCAACTAAC AGAGAAGCTG AAGAATCAGT CAGAAAGTCA TAAACAAGCC 
CAGGAGAATT TGCATGACCA GGTACAAGAG CAGAAGGCAC ATCTTAGAGC TGCACAAGAC 
CGTGTCCTTT CCCTAGAAAC TAGTGTCAAT GAATTAAATA GTCAATTAAA TGAAAGCAAG 
GAGAAGGTCT CCCAGCTTGA CATACAGATT AAAGCCAAAA CCGAACTATT ACTATCAGCA 
GAAGCAGCAA AAACTGCTCA AAGAGCTGAT CTTCAGAATC ATTTGGACAC AGCTCAAAAT 
GCATTACAAG ATAAACAGCA GGAGTTAAAT AAGATTACTA CTCAGTTGGA TCAGGTCACT 
GCAAAGTTAC AAGACAAGCA AGAACATTGC AGTCAGCTGG AAAGTCATCT TAAAGAATAT 
AAAGAGAAAT ACCTCTCTTT AGAACAGAAA ACCGAAGAGC TAGAAGGTCA AATTAAGAAA 
CTAGAAGCTG ATAGTCTTGA AGTTAAAGCA AGCAAGGAGC AGGCTTTGCA AGATCTACAA 
CAGCAAAGAC AGCTGAACAC AGATTTAGAG CTCAGAGCCA CAGAATTGAG TAAACAACTT 
GAAATGGAGA AGGAAATAGT ATCCAGTACA AGATTGGATC TACAGAAAAA ATCTGAAGCC 
CTTGAAAGTA TCAAGCAAAA GCTTACCAAG CAAGAGGAAG AAAAAAAAAT CCTGAAACAA 
GATTTTGAAA CTTTAAGTCA AGAAACAAAG ATTCAGCATG AGGAATTGAA TAACAGAATT 
CAAACAACAG TAACAGAACT ACAAAAAGTG AAAATGGAGA AAGAAGCTTT AATGACAGAG 
CTTTCTACAG TAAAGGACAA ACTATCAAAA GTTTCTGATT CTTTGAAAAA CTCTAAAAGT 
GAATTTGAAA AGGAGAATCA GAAAGGAAAA GCCGCTATAT TAGACTTGGA AAAAACTTGC 
AAAGAATTAA AGCATCAACT TCAAGTGCAG ATGGAAAACA CACTTAAGGA ACAGAAGGAA 
CTGAAAAAGT CACTTGAAAA AGAGAAGGAG GCTTCTCATC AGTTGAAATT GGAACTCAAT 
TCAATGCAGG AACAACTTAT ACAGGCCCAG AATACTTTAA AACAAAATGA AAAAGAAGAG 
CAACAACTTC AGGGGAACAT AAATGAGCTA AAGCAATCAA GTGAACAGAA GAAAAAACAA 
ATTGAAGCAC TCCAAGGAGA GCTTAAAATT GCTGTTTTAC AGAAGACAGA GCTTGAGAAT 
AAACTACAGC AGCAGTTAAC ACAGGCAGCC CAGGAACTTG CAGCAGAGAA AGAGAAAATA 
TCAGTATTAC AAAACAACTA TGAAAAAAGT CAGGAAACTT TCAAACAGCT TCAATCTGAT 
TTCTATGGGA GGGAATCTGA ACTTCTAGCC ACCAGGCAAG ATCTTAAGTC TGTAGAAGAG 
AAGCTTTCTC TAGCACAGGA GGACTTGATT TCAAACAGAA ATCAAATTGG AAATCAAAAT 
AAATTGATTC AAGAACTGAA GACTGCCAAG GCTACATTGG AGCAGGATTC AGCAAAGAAA 
GAACAGCAAT TGCAGGAGCG ATGTAAAGCA CTACAAGACA TTCAGAAAGA AAAGTCACTG 
AAAGAAAAAG AACTGGTAAA TGAGAAGTCT AAATTGGCAG AGATAGAAGA AATTAAATGT 
AGACAAGAAA AAGAAATCAC TAAACTAAAC GAAGAACTCA AGTCCCACAA ACTAGAAAGC 
ATAAAGGAGA TAACAAATCT TAAAGATGCT AAACAGCTTC TAATTCAGCA GAAATTAGAA 
CTTCAAGGAA AAGCGGACTC CCTGAAGGCA GCTGTTGAAC AGGAGAAGAG AAATCAGCAG 
ATACTAAAAG ACCAGGTGAA AAAGGAAGAA GAGGAGCTGA AGAAAGAATT TATTGAGAAA 
GAAGCTAAGT TGCATTCCGA AATAAAAGAA AAGGAAGTAG GAATGAAGAA GCATGAAGAA 
AATGAGGCTA AACTTACCAT GCAGATTACA GCATTAAATG AAAACTTAGG CACTGTGAAG 
AAGGAGTGGC AATCTAGTCA ACGGAGAGTT AGTGAGCTTG AGAAACAAAC GGATGACTTA 
CGGGGTGAAA TTGCAGTATT AGAAGCAACG GTTCAGAATA ATCAAGATGA AAGGAGAGCA 
CTACTGGAAA GATGTCTTAA AGGAGAAGGT GAAATAGAAA AGCTTCAAAC CAAAGTATTA 
GAATTGCAAA GAAAGCTGGA TAATACAACT GCAGCAGTGC AGGAGCTGGG CAGAGAAAAC 
CAATCACTTC AGATCAAACA TACACAAGCG TTGAATAGAA AGTGGGCCGA AGACAATGAA 
GTACAAAACT GTATGGCCTG TGGGAAAGGC TTTTCAGTAA CAGTGAGACG GCATCACTGC 
CGACAGTGTG GAAATATCTT CTGTGCTGAA TGTTCAGCCA AAAATGCCTT AACTCCTTCC 
TCCAAGAAGC CTGTTCGTGT CTGTGATGCA TGTTTCAATG ACTTGCAAGG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EEA1 ( XM_011538814.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011537116.1 Homo sapiens early endosome antigen 1 (EEA1), transcript variant X1, mRNA.

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CloneID	OHu61745
Clone ID Related Accession (Same CDS sequence)	XM_011538814.2
Accession Version	XM_011538814.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4362bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	early endosome antigen 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011538814.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTAAGGA GGATTTTACA GAGGACTCCT GGGAGAGTTG GCTCTCAAGG TTCTGATTTA 
GATTCATCAG CAACTCCTAT AAACACAGTG GACGTCAATA ATGAAAGCTC TTCAGAGGGT 
TTCATCTGCC CCTTATGTAT GAAAAGATGT ATAACTGCTA TTGATTTATT GGAGCATTAC 
CAGAAAGTGC ACGATGAAAA TGAAACAAGA GGACAGGAAC TTCTTAATGA CTCCAGTGTT 
ACTGGTTTCA TATGTCCCCA GTGTATGAAA TCTCTTGGAT CTGCTGATGA ACTTTTCAAA 
CATTATGAAG CTGTTCATGA TGCTGGTAAT GACTCAGGTC ATGGAGGAGA GTCTAATCTT 
GCTTTGAAGC GAGATGATGT AACACTGCTC AGACAAGAGG TCCAAGACCT ACAGGCTTCA 
CTTAAGGAAG AAAAATGGTA CTCGGAAGAA TTAAAGAAGG AATTAGAAAA ATATCAAGGG 
CTGCAGCAGC AAGAGGCCAA ACCTGATGGG TTGGTGACTG ATTCATCAGC AGAACTACAG 
TCTTTGGAAC AGCAATTAGA AGAAGCCCAA ACAGAAAATT TTAATATTAA GCAAATGAAA 
GACTTATTTG AACAGAAAGC AGCCCAACTT GCTACTGAAA TTGCAGATAT AAAGTCAAAG 
TATGATGAAG AAAGGAGTCT TCGAGAAGCT GCTGAACAAA AAGTGACACG TCTGACAGAA 
GAATTAAACA AAGAGGCAAC TGTAATTCAA GATCTGAAGA CGGAACTGCT TCAGAGACCT 
GGTATAGAAG ATGTTGCCGT GCTAAAGAAA GAACTGGTCC AAGTTCAAAC ACTAATGGAT 
AACATGACCT TGGAACGTGA GCGAGAATCT GAAAAACTCA AAGATGAATG CAAAAAATTG 
CAGTCACAAT ATGCTAGCTC AGAGGCCACA ATAAGCCAGC TAAGGAGTGA ACTTGCCAAA 
GGCCCCCAGG AAGTTGCTGT ATATGTACAG GAACTACAAA AACTGAAAAG TTCAGTTAAT 
GAATTAACAC AAAAAAATCA GACCTTGACA GAAAACTTGC TGAAAAAAGA ACAAGACTAT 
ACTAAGTTAG AGGAGAAACA TAATGAAGAA TCTGTGAGTA AAAAGAATAT TCAGGCAACC 
CTTCATCAAA AAGACCTAGA TTGTCAACAG CTTCAGTCAA GATTGTCTGC ATCTGAAACC 
TCACTGCATA GAATACATGT AGAACTAAGT GAAAAAGGAG AAGCTACTCA AAAGCTCAAA 
GAAGAATTAT CTGAGGTAGA GACCAAGTAC CAGCATCTAA AGGCGGAGTT TAAGCAGCTA 
CAACAACAGA GAGAAGAAAA GGAGCAGCAT GGGTTACAAC TCCAAAGTGA AATTAATCAA 
TTACATAGCA AACTTCTGGA GACAGAGCGC CAACTAGGGG AAGCTCATGG TAGGCTGAAG 
GAACAGAGAC AGCTTTCAAG TGAAAAGTTG ATGGATAAAG AACAACAAGT GGCTGATTTA 
CAACTCAAAC TTTCTCGGTT AGAAGAGCAG TTGAAGGAAA AAGTTACAAA TTCTACAGAA 
TTGCAGCATC AATTAGATAA AACAAAGCAA CAGCATCAAG AACAACAGGC TCTTCAGCAA 
AGCACCACGG CAAAACTTCG AGAAGCTCAG AATGATTTGG AACAAGTTCT ACGTCAAATT 
GGCGATAAGG ACCAAAAGAT CCAGAACCTT GAAGCTTTAT TACAGAAGAG TAAAGAAAAT 
ATTTCATTAC TAGAAAAAGA AAGAGAAGAT CTTTATGCAA AAATTCAGGC TGGTGAAGGA 
GAGACTGCTG TTCTTAACCA GTTACAAGAA AAAAACCATA CACTACAGGA GCAAGTAACT 
CAACTAACAG AGAAGCTGAA GAATCAGTCA GAAAGTCATA AACAAGCCCA GGAGAATTTG 
CATGACCAGG TACAAGAGCA GAAGGCACAT CTTAGAGCTG CACAAGACCG TGTCCTTTCC 
CTAGAAACTA GTGTCAATGA ATTAAATAGT CAATTAAATG AAAGCAAGGA GAAGGTCTCC 
CAGCTTGACA TACAGATTAA AGCCAAAACC GAACTATTAC TATCAGCAGA AGCAGCAAAA 
ACTGCTCAAA GAGCTGATCT TCAGAATCAT TTGGACACAG CTCAAAATGC ATTACAAGAT 
AAACAGCAGG AGTTAAATAA GATTACTACT CAGTTGGATC AGGTCACTGC AAAGTTACAA 
GACAAGCAAG AACATTGCAG TCAGCTGGAA AGTCATCTTA AAGAATATAA AGAGAAATAC 
CTCTCTTTAG AACAGAAAAC CGAAGAGCTA GAAGGTCAAA TTAAGAAACT AGAAGCTGAT 
AGTCTTGAAG TTAAAGCAAG CAAGGAGCAG GCTTTGCAAG ATCTACAACA GCAAAGACAG 
CTGAACACAG ATTTAGAGCT CAGAGCCACA GAATTGAGTA AACAACTTGA AATGGAGAAG 
GAAATAGTAT CCAGTACAAG ATTGGATCTA CAGAAAAAAT CTGAAGCCCT TGAAAGTATC 
AAGCAAAAGC TTACCAAGCA AGAGGAAGAA AAAAAAATCC TGAAACAAGA TTTTGAAACT 
TTAAGTCAAG AAACAAAGAT TCAGCATGAG GAATTGAATA ACAGAATTCA AACAACAGTA 
ACAGAACTAC AAAAAGTGAA AATGGAGAAA GAAGCTTTAA TGACAGAGCT TTCTACAGTA 
AAGGACAAAC TATCAAAAGT TTCTGATTCT TTGAAAAACT CTAAAAGTGA ATTTGAAAAG 
GAGAATCAGA AAGGAAAAGC CGCTATATTA GACTTGGAAA AAACTTGCAA AGAATTAAAG 
CATCAACTTC AAGTGCAGAT GGAAAACACA CTTAAGGAAC AGAAGGAACT GAAAAAGTCA 
CTTGAAAAAG AGAAGGAGGC TTCTCATCAG TTGAAATTGG AACTCAATTC AATGCAGGAA 
CAACTTATAC AGGCCCAGAA TACTTTAAAA CAAAATGAAA AAGAAGAGCA ACAACTTCAG 
GGGAACATAA ATGAGCTAAA GCAATCAAGT GAACAGAAGA AAAAACAAAT TGAAGCACTC 
CAAGGAGAGC TTAAAATTGC TGTTTTACAG AAGACAGAGC TTGAGAATAA ACTACAGCAG 
CAGTTAACAC AGGCAGCCCA GGAACTTGCA GCAGAGAAAG AGAAAATATC AGTATTACAA 
AACAACTATG AAAAAAGTCA GGAAACTTTC AAACAGCTTC AATCTGATTT CTATGGGAGG 
GAATCTGAAC TTCTAGCCAC CAGGCAAGAT CTTAAGTCTG TAGAAGAGAA GCTTTCTCTA 
GCACAGGAGG ACTTGATTTC AAACAGAAAT CAAATTGGAA ATCAAAATAA ATTGATTCAA 
GAACTGAAGA CTGCCAAGGC TACATTGGAG CAGGATTCAG CAAAGAAAGA ACAGCAATTG 
CAGGAGCGAT GTAAAGCACT ACAAGACATT CAGAAAGAAA AGTCACTGAA AGAAAAAGAA 
CTGGTAAATG AGAAGTCTAA ATTGGCAGAG ATAGAAGAAA TTAAATGTAG ACAAGAAAAA 
GAAATCACTA AACTAAACGA AGAACTCAAG TCCCACAAAC TAGAAAGCAT AAAGGAGATA 
ACAAATCTTA AAGATGCTAA ACAGCTTCTA ATTCAGCAGA AATTAGAACT TCAAGGAAAA 
GCGGACTCCC TGAAGGCAGC TGTTGAACAG GAGAAGAGAA ATCAGCAGAT ACTAAAAGAC 
CAGGTGAAAA AGGAAGAAGA GGAGCTGAAG AAAGAATTTA TTGAGAAAGA AGCTAAGTTG 
CATTCCGAAA TAAAAGAAAA GGAAGTAGGA ATGAAGAAGC ATGAAGAAAA TGAGGCTAAA 
CTTACCATGC AGATTACAGC ATTAAATGAA AACTTAGGCA CTGTGAAGAA GGAGTGGCAA 
TCTAGTCAAC GGAGAGTTAG TGAGCTTGAG AAACAAACGG ATGACTTACG GGGTGAAATT 
GCAGTATTAG AAGCAACGGT TCAGAATAAT CAAGATGAAA GGAGAGCACT ACTGGAAAGA 
TGTCTTAAAG GAGAAGGTGA AATAGAAAAG CTTCAAACCA AAGTATTAGA ATTGCAAAGA 
AAGCTGGATA ATACAACTGC AGCAGTGCAG GAGCTGGGCA GAGAAAACCA ATCACTTCAG 
ATCAAACATA CACAAGCGTT GAATAGAAAG TGGGCCGAAG ACAATGAAGT ACAAAACTGT 
ATGGCCTGTG GGAAAGGCTT TTCAGTAACA GTGAGACGGC ATCACTGCCG ACAGTGTGGA 
AATATCTTCT GTGCTGAATG TTCAGCCAAA AATGCCTTAA CTCCTTCCTC CAAGAAGCCT 
GTTCGTGTCT GTGATGCATG TTTCAATGAC TTGCAAGGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011537116.1
CDS	246..4607
Translation	MLRRILQRTPGRVGSQGSDLDSSATPINTVDVNNESSSEGFICPLCMKRCITAIDLLEHYQKVHDENETRGQELLNDSSVTGFICPQCMKSLGSADELFKHYEAVHDAGNDSGHGGESNLALKRDDVTLLRQEVQDLQASLKEEKWYSEELKKELEKYQGLQQQEAKPDGLVTDSSAELQSLEQQLEEAQTENFNIKQMKDLFEQKAAQLATEIADIKSKYDEERSLREAAEQKVTRLTEELNKEATVIQDLKTELLQRPGIEDVAVLKKELVQVQTLMDNMTLERERESEKLKDECKKLQSQYASSEATISQLRSELAKGPQEVAVYVQELQKLKSSVNELTQKNQTLTENLLKKEQDYTKLEEKHNEESVSKKNIQATLHQKDLDCQQLQSRLSASETSLHRIHVELSEKGEATQKLKEELSEVETKYQHLKAEFKQLQQQREEKEQHGLQLQSEINQLHSKLLETERQLGEAHGRLKEQRQLSSEKLMDKEQQVADLQLKLSRLEEQLKEKVTNSTELQHQLDKTKQQHQEQQALQQSTTAKLREAQNDLEQVLRQIGDKDQKIQNLEALLQKSKENISLLEKEREDLYAKIQAGEGETAVLNQLQEKNHTLQEQVTQLTEKLKNQSESHKQAQENLHDQVQEQKAHLRAAQDRVLSLETSVNELNSQLNESKEKVSQLDIQIKAKTELLLSAEAAKTAQRADLQNHLDTAQNALQDKQQELNKITTQLDQVTAKLQDKQEHCSQLESHLKEYKEKYLSLEQKTEELEGQIKKLEADSLEVKASKEQALQDLQQQRQLNTDLELRATELSKQLEMEKEIVSSTRLDLQKKSEALESIKQKLTKQEEEKKILKQDFETLSQETKIQHEELNNRIQTTVTELQKVKMEKEALMTELSTVKDKLSKVSDSLKNSKSEFEKENQKGKAAILDLEKTCKELKHQLQVQMENTLKEQKELKKSLEKEKEASHQLKLELNSMQEQLIQAQNTLKQNEKEEQQLQGNINELKQSSEQKKKQIEALQGELKIAVLQKTELENKLQQQLTQAAQELAAEKEKISVLQNNYEKSQETFKQLQSDFYGRESELLATRQDLKSVEEKLSLAQEDLISNRNQIGNQNKLIQELKTAKATLEQDSAKKEQQLQERCKALQDIQKEKSLKEKELVNEKSKLAEIEEIKCRQEKEITKLNEELKSHKLESIKEITNLKDAKQLLIQQKLELQGKADSLKAAVEQEKRNQQILKDQVKKEEEELKKEFIEKEAKLHSEIKEKEVGMKKHEENEAKLTMQITALNENLGTVKKEWQSSQRRVSELEKQTDDLRGEIAVLEATVQNNQDERRALLERCLKGEGEIEKLQTKVLELQRKLDNTTAAVQELGRENQSLQIKHTQALNRKWAEDNEVQNCMACGKGFSVTVRRHHCRQCGNIFCAECSAKNALTPSSKKPVRVCDACFNDLQG

Target ORF information:

RefSeq Version	XM_011538814.2
Organism	Homo sapiens(human)
Definition	Homo sapiens early endosome antigen 1 (EEA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011538814.2

ORF Insert Sequence:

ATGTTAAGGA GGATTTTACA GAGGACTCCT GGGAGAGTTG GCTCTCAAGG TTCTGATTTA 
GATTCATCAG CAACTCCTAT AAACACAGTG GACGTCAATA ATGAAAGCTC TTCAGAGGGT 
TTCATCTGCC CCTTATGTAT GAAAAGATGT ATAACTGCTA TTGATTTATT GGAGCATTAC 
CAGAAAGTGC ACGATGAAAA TGAAACAAGA GGACAGGAAC TTCTTAATGA CTCCAGTGTT 
ACTGGTTTCA TATGTCCCCA GTGTATGAAA TCTCTTGGAT CTGCTGATGA ACTTTTCAAA 
CATTATGAAG CTGTTCATGA TGCTGGTAAT GACTCAGGTC ATGGAGGAGA GTCTAATCTT 
GCTTTGAAGC GAGATGATGT AACACTGCTC AGACAAGAGG TCCAAGACCT ACAGGCTTCA 
CTTAAGGAAG AAAAATGGTA CTCGGAAGAA TTAAAGAAGG AATTAGAAAA ATATCAAGGG 
CTGCAGCAGC AAGAGGCCAA ACCTGATGGG TTGGTGACTG ATTCATCAGC AGAACTACAG 
TCTTTGGAAC AGCAATTAGA AGAAGCCCAA ACAGAAAATT TTAATATTAA GCAAATGAAA 
GACTTATTTG AACAGAAAGC AGCCCAACTT GCTACTGAAA TTGCAGATAT AAAGTCAAAG 
TATGATGAAG AAAGGAGTCT TCGAGAAGCT GCTGAACAAA AAGTGACACG TCTGACAGAA 
GAATTAAACA AAGAGGCAAC TGTAATTCAA GATCTGAAGA CGGAACTGCT TCAGAGACCT 
GGTATAGAAG ATGTTGCCGT GCTAAAGAAA GAACTGGTCC AAGTTCAAAC ACTAATGGAT 
AACATGACCT TGGAACGTGA GCGAGAATCT GAAAAACTCA AAGATGAATG CAAAAAATTG 
CAGTCACAAT ATGCTAGCTC AGAGGCCACA ATAAGCCAGC TAAGGAGTGA ACTTGCCAAA 
GGCCCCCAGG AAGTTGCTGT ATATGTACAG GAACTACAAA AACTGAAAAG TTCAGTTAAT 
GAATTAACAC AAAAAAATCA GACCTTGACA GAAAACTTGC TGAAAAAAGA ACAAGACTAT 
ACTAAGTTAG AGGAGAAACA TAATGAAGAA TCTGTGAGTA AAAAGAATAT TCAGGCAACC 
CTTCATCAAA AAGACCTAGA TTGTCAACAG CTTCAGTCAA GATTGTCTGC ATCTGAAACC 
TCACTGCATA GAATACATGT AGAACTAAGT GAAAAAGGAG AAGCTACTCA AAAGCTCAAA 
GAAGAATTAT CTGAGGTAGA GACCAAGTAC CAGCATCTAA AGGCGGAGTT TAAGCAGCTA 
CAACAACAGA GAGAAGAAAA GGAGCAGCAT GGGTTACAAC TCCAAAGTGA AATTAATCAA 
TTACATAGCA AACTTCTGGA GACAGAGCGC CAACTAGGGG AAGCTCATGG TAGGCTGAAG 
GAACAGAGAC AGCTTTCAAG TGAAAAGTTG ATGGATAAAG AACAACAAGT GGCTGATTTA 
CAACTCAAAC TTTCTCGGTT AGAAGAGCAG TTGAAGGAAA AAGTTACAAA TTCTACAGAA 
TTGCAGCATC AATTAGATAA AACAAAGCAA CAGCATCAAG AACAACAGGC TCTTCAGCAA 
AGCACCACGG CAAAACTTCG AGAAGCTCAG AATGATTTGG AACAAGTTCT ACGTCAAATT 
GGCGATAAGG ACCAAAAGAT CCAGAACCTT GAAGCTTTAT TACAGAAGAG TAAAGAAAAT 
ATTTCATTAC TAGAAAAAGA AAGAGAAGAT CTTTATGCAA AAATTCAGGC TGGTGAAGGA 
GAGACTGCTG TTCTTAACCA GTTACAAGAA AAAAACCATA CACTACAGGA GCAAGTAACT 
CAACTAACAG AGAAGCTGAA GAATCAGTCA GAAAGTCATA AACAAGCCCA GGAGAATTTG 
CATGACCAGG TACAAGAGCA GAAGGCACAT CTTAGAGCTG CACAAGACCG TGTCCTTTCC 
CTAGAAACTA GTGTCAATGA ATTAAATAGT CAATTAAATG AAAGCAAGGA GAAGGTCTCC 
CAGCTTGACA TACAGATTAA AGCCAAAACC GAACTATTAC TATCAGCAGA AGCAGCAAAA 
ACTGCTCAAA GAGCTGATCT TCAGAATCAT TTGGACACAG CTCAAAATGC ATTACAAGAT 
AAACAGCAGG AGTTAAATAA GATTACTACT CAGTTGGATC AGGTCACTGC AAAGTTACAA 
GACAAGCAAG AACATTGCAG TCAGCTGGAA AGTCATCTTA AAGAATATAA AGAGAAATAC 
CTCTCTTTAG AACAGAAAAC CGAAGAGCTA GAAGGTCAAA TTAAGAAACT AGAAGCTGAT 
AGTCTTGAAG TTAAAGCAAG CAAGGAGCAG GCTTTGCAAG ATCTACAACA GCAAAGACAG 
CTGAACACAG ATTTAGAGCT CAGAGCCACA GAATTGAGTA AACAACTTGA AATGGAGAAG 
GAAATAGTAT CCAGTACAAG ATTGGATCTA CAGAAAAAAT CTGAAGCCCT TGAAAGTATC 
AAGCAAAAGC TTACCAAGCA AGAGGAAGAA AAAAAAATCC TGAAACAAGA TTTTGAAACT 
TTAAGTCAAG AAACAAAGAT TCAGCATGAG GAATTGAATA ACAGAATTCA AACAACAGTA 
ACAGAACTAC AAAAAGTGAA AATGGAGAAA GAAGCTTTAA TGACAGAGCT TTCTACAGTA 
AAGGACAAAC TATCAAAAGT TTCTGATTCT TTGAAAAACT CTAAAAGTGA ATTTGAAAAG 
GAGAATCAGA AAGGAAAAGC CGCTATATTA GACTTGGAAA AAACTTGCAA AGAATTAAAG 
CATCAACTTC AAGTGCAGAT GGAAAACACA CTTAAGGAAC AGAAGGAACT GAAAAAGTCA 
CTTGAAAAAG AGAAGGAGGC TTCTCATCAG TTGAAATTGG AACTCAATTC AATGCAGGAA 
CAACTTATAC AGGCCCAGAA TACTTTAAAA CAAAATGAAA AAGAAGAGCA ACAACTTCAG 
GGGAACATAA ATGAGCTAAA GCAATCAAGT GAACAGAAGA AAAAACAAAT TGAAGCACTC 
CAAGGAGAGC TTAAAATTGC TGTTTTACAG AAGACAGAGC TTGAGAATAA ACTACAGCAG 
CAGTTAACAC AGGCAGCCCA GGAACTTGCA GCAGAGAAAG AGAAAATATC AGTATTACAA 
AACAACTATG AAAAAAGTCA GGAAACTTTC AAACAGCTTC AATCTGATTT CTATGGGAGG 
GAATCTGAAC TTCTAGCCAC CAGGCAAGAT CTTAAGTCTG TAGAAGAGAA GCTTTCTCTA 
GCACAGGAGG ACTTGATTTC AAACAGAAAT CAAATTGGAA ATCAAAATAA ATTGATTCAA 
GAACTGAAGA CTGCCAAGGC TACATTGGAG CAGGATTCAG CAAAGAAAGA ACAGCAATTG 
CAGGAGCGAT GTAAAGCACT ACAAGACATT CAGAAAGAAA AGTCACTGAA AGAAAAAGAA 
CTGGTAAATG AGAAGTCTAA ATTGGCAGAG ATAGAAGAAA TTAAATGTAG ACAAGAAAAA 
GAAATCACTA AACTAAACGA AGAACTCAAG TCCCACAAAC TAGAAAGCAT AAAGGAGATA 
ACAAATCTTA AAGATGCTAA ACAGCTTCTA ATTCAGCAGA AATTAGAACT TCAAGGAAAA 
GCGGACTCCC TGAAGGCAGC TGTTGAACAG GAGAAGAGAA ATCAGCAGAT ACTAAAAGAC 
CAGGTGAAAA AGGAAGAAGA GGAGCTGAAG AAAGAATTTA TTGAGAAAGA AGCTAAGTTG 
CATTCCGAAA TAAAAGAAAA GGAAGTAGGA ATGAAGAAGC ATGAAGAAAA TGAGGCTAAA 
CTTACCATGC AGATTACAGC ATTAAATGAA AACTTAGGCA CTGTGAAGAA GGAGTGGCAA 
TCTAGTCAAC GGAGAGTTAG TGAGCTTGAG AAACAAACGG ATGACTTACG GGGTGAAATT 
GCAGTATTAG AAGCAACGGT TCAGAATAAT CAAGATGAAA GGAGAGCACT ACTGGAAAGA 
TGTCTTAAAG GAGAAGGTGA AATAGAAAAG CTTCAAACCA AAGTATTAGA ATTGCAAAGA 
AAGCTGGATA ATACAACTGC AGCAGTGCAG GAGCTGGGCA GAGAAAACCA ATCACTTCAG 
ATCAAACATA CACAAGCGTT GAATAGAAAG TGGGCCGAAG ACAATGAAGT ACAAAACTGT 
ATGGCCTGTG GGAAAGGCTT TTCAGTAACA GTGAGACGGC ATCACTGCCG ACAGTGTGGA 
AATATCTTCT GTGCTGAATG TTCAGCCAAA AATGCCTTAA CTCCTTCCTC CAAGAAGCCT 
GTTCGTGTCT GTGATGCATG TTTCAATGAC TTGCAAGGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EEA1 ( XM_011538815.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011537117.1 Homo sapiens early endosome antigen 1 (EEA1), transcript variant X3, mRNA.

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CloneID	OHu61746
Clone ID Related Accession (Same CDS sequence)	XM_011538815.2
Accession Version	XM_011538815.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4098bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	early endosome antigen 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011538815.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCTC TTGGATCTGC TGATGAACTT TTCAAACATT ATGAAGCTGT TCATGATGCT 
GGTAATGACT CAGGTCATGG AGGAGAGTCT AATCTTGCTT TGAAGCGAGA TGATGTAACA 
CTGCTCAGAC AAGAGGTCCA AGACCTACAG GCTTCACTTA AGGAAGAAAA ATGGTACTCG 
GAAGAATTAA AGAAGGAATT AGAAAAATAT CAAGGGCTGC AGCAGCAAGA GGCCAAACCT 
GATGGGTTGG TGACTGATTC ATCAGCAGAA CTACAGTCTT TGGAACAGCA ATTAGAAGAA 
GCCCAAACAG AAAATTTTAA TATTAAGCAA ATGAAAGACT TATTTGAACA GAAAGCAGCC 
CAACTTGCTA CTGAAATTGC AGATATAAAG TCAAAGTATG ATGAAGAAAG GAGTCTTCGA 
GAAGCTGCTG AACAAAAAGT GACACGTCTG ACAGAAGAAT TAAACAAAGA GGCAACTGTA 
ATTCAAGATC TGAAGACGGA ACTGCTTCAG AGACCTGGTA TAGAAGATGT TGCCGTGCTA 
AAGAAAGAAC TGGTCCAAGT TCAAACACTA ATGGATAACA TGACCTTGGA ACGTGAGCGA 
GAATCTGAAA AACTCAAAGA TGAATGCAAA AAATTGCAGT CACAATATGC TAGCTCAGAG 
GCCACAATAA GCCAGCTAAG GAGTGAACTT GCCAAAGGCC CCCAGGAAGT TGCTGTATAT 
GTACAGGAAC TACAAAAACT GAAAAGTTCA GTTAATGAAT TAACACAAAA AAATCAGACC 
TTGACAGAAA ACTTGCTGAA AAAAGAACAA GACTATACTA AGTTAGAGGA GAAACATAAT 
GAAGAATCTG TGAGTAAAAA GAATATTCAG GCAACCCTTC ATCAAAAAGA CCTAGATTGT 
CAACAGCTTC AGTCAAGATT GTCTGCATCT GAAACCTCAC TGCATAGAAT ACATGTAGAA 
CTAAGTGAAA AAGGAGAAGC TACTCAAAAG CTCAAAGAAG AATTATCTGA GGTAGAGACC 
AAGTACCAGC ATCTAAAGGC GGAGTTTAAG CAGCTACAAC AACAGAGAGA AGAAAAGGAG 
CAGCATGGGT TACAACTCCA AAGTGAAATT AATCAATTAC ATAGCAAACT TCTGGAGACA 
GAGCGCCAAC TAGGGGAAGC TCATGGTAGG CTGAAGGAAC AGAGACAGCT TTCAAGTGAA 
AAGTTGATGG ATAAAGAACA ACAAGTGGCT GATTTACAAC TCAAACTTTC TCGGTTAGAA 
GAGCAGTTGA AGGAAAAAGT TACAAATTCT ACAGAATTGC AGCATCAATT AGATAAAACA 
AAGCAACAGC ATCAAGAACA ACAGGCTCTT CAGCAAAGCA CCACGGCAAA ACTTCGAGAA 
GCTCAGAATG ATTTGGAACA AGTTCTACGT CAAATTGGCG ATAAGGACCA AAAGATCCAG 
AACCTTGAAG CTTTATTACA GAAGAGTAAA GAAAATATTT CATTACTAGA AAAAGAAAGA 
GAAGATCTTT ATGCAAAAAT TCAGGCTGGT GAAGGAGAGA CTGCTGTTCT TAACCAGTTA 
CAAGAAAAAA ACCATACACT ACAGGAGCAA GTAACTCAAC TAACAGAGAA GCTGAAGAAT 
CAGTCAGAAA GTCATAAACA AGCCCAGGAG AATTTGCATG ACCAGGTACA AGAGCAGAAG 
GCACATCTTA GAGCTGCACA AGACCGTGTC CTTTCCCTAG AAACTAGTGT CAATGAATTA 
AATAGTCAAT TAAATGAAAG CAAGGAGAAG GTCTCCCAGC TTGACATACA GATTAAAGCC 
AAAACCGAAC TATTACTATC AGCAGAAGCA GCAAAAACTG CTCAAAGAGC TGATCTTCAG 
AATCATTTGG ACACAGCTCA AAATGCATTA CAAGATAAAC AGCAGGAGTT AAATAAGATT 
ACTACTCAGT TGGATCAGGT CACTGCAAAG TTACAAGACA AGCAAGAACA TTGCAGTCAG 
CTGGAAAGTC ATCTTAAAGA ATATAAAGAG AAATACCTCT CTTTAGAACA GAAAACCGAA 
GAGCTAGAAG GTCAAATTAA GAAACTAGAA GCTGATAGTC TTGAAGTTAA AGCAAGCAAG 
GAGCAGGCTT TGCAAGATCT ACAACAGCAA AGACAGCTGA ACACAGATTT AGAGCTCAGA 
GCCACAGAAT TGAGTAAACA ACTTGAAATG GAGAAGGAAA TAGTATCCAG TACAAGATTG 
GATCTACAGA AAAAATCTGA AGCCCTTGAA AGTATCAAGC AAAAGCTTAC CAAGCAAGAG 
GAAGAAAAAA AAATCCTGAA ACAAGATTTT GAAACTTTAA GTCAAGAAAC AAAGATTCAG 
CATGAGGAAT TGAATAACAG AATTCAAACA ACAGTAACAG AACTACAAAA AGTGAAAATG 
GAGAAAGAAG CTTTAATGAC AGAGCTTTCT ACAGTAAAGG ACAAACTATC AAAAGTTTCT 
GATTCTTTGA AAAACTCTAA AAGTGAATTT GAAAAGGAGA ATCAGAAAGG AAAAGCCGCT 
ATATTAGACT TGGAAAAAAC TTGCAAAGAA TTAAAGCATC AACTTCAAGT GCAGATGGAA 
AACACACTTA AGGAACAGAA GGAACTGAAA AAGTCACTTG AAAAAGAGAA GGAGGCTTCT 
CATCAGTTGA AATTGGAACT CAATTCAATG CAGGAACAAC TTATACAGGC CCAGAATACT 
TTAAAACAAA ATGAAAAAGA AGAGCAACAA CTTCAGGGGA ACATAAATGA GCTAAAGCAA 
TCAAGTGAAC AGAAGAAAAA ACAAATTGAA GCACTCCAAG GAGAGCTTAA AATTGCTGTT 
TTACAGAAGA CAGAGCTTGA GAATAAACTA CAGCAGCAGT TAACACAGGC AGCCCAGGAA 
CTTGCAGCAG AGAAAGAGAA AATATCAGTA TTACAAAACA ACTATGAAAA AAGTCAGGAA 
ACTTTCAAAC AGCTTCAATC TGATTTCTAT GGGAGGGAAT CTGAACTTCT AGCCACCAGG 
CAAGATCTTA AGTCTGTAGA AGAGAAGCTT TCTCTAGCAC AGGAGGACTT GATTTCAAAC 
AGAAATCAAA TTGGAAATCA AAATAAATTG ATTCAAGAAC TGAAGACTGC CAAGGCTACA 
TTGGAGCAGG ATTCAGCAAA GAAAGAACAG CAATTGCAGG AGCGATGTAA AGCACTACAA 
GACATTCAGA AAGAAAAGTC ACTGAAAGAA AAAGAACTGG TAAATGAGAA GTCTAAATTG 
GCAGAGATAG AAGAAATTAA ATGTAGACAA GAAAAAGAAA TCACTAAACT AAACGAAGAA 
CTCAAGTCCC ACAAACTAGA AAGCATAAAG GAGATAACAA ATCTTAAAGA TGCTAAACAG 
CTTCTAATTC AGCAGAAATT AGAACTTCAA GGAAAAGCGG ACTCCCTGAA GGCAGCTGTT 
GAACAGGAGA AGAGAAATCA GCAGATACTA AAAGACCAGG TGAAAAAGGA AGAAGAGGAG 
CTGAAGAAAG AATTTATTGA GAAAGAAGCT AAGTTGCATT CCGAAATAAA AGAAAAGGAA 
GTAGGAATGA AGAAGCATGA AGAAAATGAG GCTAAACTTA CCATGCAGAT TACAGCATTA 
AATGAAAACT TAGGCACTGT GAAGAAGGAG TGGCAATCTA GTCAACGGAG AGTTAGTGAG 
CTTGAGAAAC AAACGGATGA CTTACGGGGT GAAATTGCAG TATTAGAAGC AACGGTTCAG 
AATAATCAAG ATGAAAGGAG AGCACTACTG GAAAGATGTC TTAAAGGAGA AGGTGAAATA 
GAAAAGCTTC AAACCAAAGT ATTAGAATTG CAAAGAAAGC TGGATAATAC AACTGCAGCA 
GTGCAGGAGC TGGGCAGAGA AAACCAATCA CTTCAGATCA AACATACACA AGCGTTGAAT 
AGAAAGTGGG CCGAAGACAA TGAAGTACAA AACTGTATGG CCTGTGGGAA AGGCTTTTCA 
GTAACAGTGA GACGGCATCA CTGCCGACAG TGTGGAAATA TCTTCTGTGC TGAATGTTCA 
GCCAAAAATG CCTTAACTCC TTCCTCCAAG AAGCCTGTTC GTGTCTGTGA TGCATGTTTC 
AATGACTTGC AAGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011537117.1
CDS	221..4318
Translation	MKSLGSADELFKHYEAVHDAGNDSGHGGESNLALKRDDVTLLRQEVQDLQASLKEEKWYSEELKKELEKYQGLQQQEAKPDGLVTDSSAELQSLEQQLEEAQTENFNIKQMKDLFEQKAAQLATEIADIKSKYDEERSLREAAEQKVTRLTEELNKEATVIQDLKTELLQRPGIEDVAVLKKELVQVQTLMDNMTLERERESEKLKDECKKLQSQYASSEATISQLRSELAKGPQEVAVYVQELQKLKSSVNELTQKNQTLTENLLKKEQDYTKLEEKHNEESVSKKNIQATLHQKDLDCQQLQSRLSASETSLHRIHVELSEKGEATQKLKEELSEVETKYQHLKAEFKQLQQQREEKEQHGLQLQSEINQLHSKLLETERQLGEAHGRLKEQRQLSSEKLMDKEQQVADLQLKLSRLEEQLKEKVTNSTELQHQLDKTKQQHQEQQALQQSTTAKLREAQNDLEQVLRQIGDKDQKIQNLEALLQKSKENISLLEKEREDLYAKIQAGEGETAVLNQLQEKNHTLQEQVTQLTEKLKNQSESHKQAQENLHDQVQEQKAHLRAAQDRVLSLETSVNELNSQLNESKEKVSQLDIQIKAKTELLLSAEAAKTAQRADLQNHLDTAQNALQDKQQELNKITTQLDQVTAKLQDKQEHCSQLESHLKEYKEKYLSLEQKTEELEGQIKKLEADSLEVKASKEQALQDLQQQRQLNTDLELRATELSKQLEMEKEIVSSTRLDLQKKSEALESIKQKLTKQEEEKKILKQDFETLSQETKIQHEELNNRIQTTVTELQKVKMEKEALMTELSTVKDKLSKVSDSLKNSKSEFEKENQKGKAAILDLEKTCKELKHQLQVQMENTLKEQKELKKSLEKEKEASHQLKLELNSMQEQLIQAQNTLKQNEKEEQQLQGNINELKQSSEQKKKQIEALQGELKIAVLQKTELENKLQQQLTQAAQELAAEKEKISVLQNNYEKSQETFKQLQSDFYGRESELLATRQDLKSVEEKLSLAQEDLISNRNQIGNQNKLIQELKTAKATLEQDSAKKEQQLQERCKALQDIQKEKSLKEKELVNEKSKLAEIEEIKCRQEKEITKLNEELKSHKLESIKEITNLKDAKQLLIQQKLELQGKADSLKAAVEQEKRNQQILKDQVKKEEEELKKEFIEKEAKLHSEIKEKEVGMKKHEENEAKLTMQITALNENLGTVKKEWQSSQRRVSELEKQTDDLRGEIAVLEATVQNNQDERRALLERCLKGEGEIEKLQTKVLELQRKLDNTTAAVQELGRENQSLQIKHTQALNRKWAEDNEVQNCMACGKGFSVTVRRHHCRQCGNIFCAECSAKNALTPSSKKPVRVCDACFNDLQG

Target ORF information:

RefSeq Version	XM_011538815.2
Organism	Homo sapiens(human)
Definition	Homo sapiens early endosome antigen 1 (EEA1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011538815.2

ORF Insert Sequence:

ATGAAATCTC TTGGATCTGC TGATGAACTT TTCAAACATT ATGAAGCTGT TCATGATGCT 
GGTAATGACT CAGGTCATGG AGGAGAGTCT AATCTTGCTT TGAAGCGAGA TGATGTAACA 
CTGCTCAGAC AAGAGGTCCA AGACCTACAG GCTTCACTTA AGGAAGAAAA ATGGTACTCG 
GAAGAATTAA AGAAGGAATT AGAAAAATAT CAAGGGCTGC AGCAGCAAGA GGCCAAACCT 
GATGGGTTGG TGACTGATTC ATCAGCAGAA CTACAGTCTT TGGAACAGCA ATTAGAAGAA 
GCCCAAACAG AAAATTTTAA TATTAAGCAA ATGAAAGACT TATTTGAACA GAAAGCAGCC 
CAACTTGCTA CTGAAATTGC AGATATAAAG TCAAAGTATG ATGAAGAAAG GAGTCTTCGA 
GAAGCTGCTG AACAAAAAGT GACACGTCTG ACAGAAGAAT TAAACAAAGA GGCAACTGTA 
ATTCAAGATC TGAAGACGGA ACTGCTTCAG AGACCTGGTA TAGAAGATGT TGCCGTGCTA 
AAGAAAGAAC TGGTCCAAGT TCAAACACTA ATGGATAACA TGACCTTGGA ACGTGAGCGA 
GAATCTGAAA AACTCAAAGA TGAATGCAAA AAATTGCAGT CACAATATGC TAGCTCAGAG 
GCCACAATAA GCCAGCTAAG GAGTGAACTT GCCAAAGGCC CCCAGGAAGT TGCTGTATAT 
GTACAGGAAC TACAAAAACT GAAAAGTTCA GTTAATGAAT TAACACAAAA AAATCAGACC 
TTGACAGAAA ACTTGCTGAA AAAAGAACAA GACTATACTA AGTTAGAGGA GAAACATAAT 
GAAGAATCTG TGAGTAAAAA GAATATTCAG GCAACCCTTC ATCAAAAAGA CCTAGATTGT 
CAACAGCTTC AGTCAAGATT GTCTGCATCT GAAACCTCAC TGCATAGAAT ACATGTAGAA 
CTAAGTGAAA AAGGAGAAGC TACTCAAAAG CTCAAAGAAG AATTATCTGA GGTAGAGACC 
AAGTACCAGC ATCTAAAGGC GGAGTTTAAG CAGCTACAAC AACAGAGAGA AGAAAAGGAG 
CAGCATGGGT TACAACTCCA AAGTGAAATT AATCAATTAC ATAGCAAACT TCTGGAGACA 
GAGCGCCAAC TAGGGGAAGC TCATGGTAGG CTGAAGGAAC AGAGACAGCT TTCAAGTGAA 
AAGTTGATGG ATAAAGAACA ACAAGTGGCT GATTTACAAC TCAAACTTTC TCGGTTAGAA 
GAGCAGTTGA AGGAAAAAGT TACAAATTCT ACAGAATTGC AGCATCAATT AGATAAAACA 
AAGCAACAGC ATCAAGAACA ACAGGCTCTT CAGCAAAGCA CCACGGCAAA ACTTCGAGAA 
GCTCAGAATG ATTTGGAACA AGTTCTACGT CAAATTGGCG ATAAGGACCA AAAGATCCAG 
AACCTTGAAG CTTTATTACA GAAGAGTAAA GAAAATATTT CATTACTAGA AAAAGAAAGA 
GAAGATCTTT ATGCAAAAAT TCAGGCTGGT GAAGGAGAGA CTGCTGTTCT TAACCAGTTA 
CAAGAAAAAA ACCATACACT ACAGGAGCAA GTAACTCAAC TAACAGAGAA GCTGAAGAAT 
CAGTCAGAAA GTCATAAACA AGCCCAGGAG AATTTGCATG ACCAGGTACA AGAGCAGAAG 
GCACATCTTA GAGCTGCACA AGACCGTGTC CTTTCCCTAG AAACTAGTGT CAATGAATTA 
AATAGTCAAT TAAATGAAAG CAAGGAGAAG GTCTCCCAGC TTGACATACA GATTAAAGCC 
AAAACCGAAC TATTACTATC AGCAGAAGCA GCAAAAACTG CTCAAAGAGC TGATCTTCAG 
AATCATTTGG ACACAGCTCA AAATGCATTA CAAGATAAAC AGCAGGAGTT AAATAAGATT 
ACTACTCAGT TGGATCAGGT CACTGCAAAG TTACAAGACA AGCAAGAACA TTGCAGTCAG 
CTGGAAAGTC ATCTTAAAGA ATATAAAGAG AAATACCTCT CTTTAGAACA GAAAACCGAA 
GAGCTAGAAG GTCAAATTAA GAAACTAGAA GCTGATAGTC TTGAAGTTAA AGCAAGCAAG 
GAGCAGGCTT TGCAAGATCT ACAACAGCAA AGACAGCTGA ACACAGATTT AGAGCTCAGA 
GCCACAGAAT TGAGTAAACA ACTTGAAATG GAGAAGGAAA TAGTATCCAG TACAAGATTG 
GATCTACAGA AAAAATCTGA AGCCCTTGAA AGTATCAAGC AAAAGCTTAC CAAGCAAGAG 
GAAGAAAAAA AAATCCTGAA ACAAGATTTT GAAACTTTAA GTCAAGAAAC AAAGATTCAG 
CATGAGGAAT TGAATAACAG AATTCAAACA ACAGTAACAG AACTACAAAA AGTGAAAATG 
GAGAAAGAAG CTTTAATGAC AGAGCTTTCT ACAGTAAAGG ACAAACTATC AAAAGTTTCT 
GATTCTTTGA AAAACTCTAA AAGTGAATTT GAAAAGGAGA ATCAGAAAGG AAAAGCCGCT 
ATATTAGACT TGGAAAAAAC TTGCAAAGAA TTAAAGCATC AACTTCAAGT GCAGATGGAA 
AACACACTTA AGGAACAGAA GGAACTGAAA AAGTCACTTG AAAAAGAGAA GGAGGCTTCT 
CATCAGTTGA AATTGGAACT CAATTCAATG CAGGAACAAC TTATACAGGC CCAGAATACT 
TTAAAACAAA ATGAAAAAGA AGAGCAACAA CTTCAGGGGA ACATAAATGA GCTAAAGCAA 
TCAAGTGAAC AGAAGAAAAA ACAAATTGAA GCACTCCAAG GAGAGCTTAA AATTGCTGTT 
TTACAGAAGA CAGAGCTTGA GAATAAACTA CAGCAGCAGT TAACACAGGC AGCCCAGGAA 
CTTGCAGCAG AGAAAGAGAA AATATCAGTA TTACAAAACA ACTATGAAAA AAGTCAGGAA 
ACTTTCAAAC AGCTTCAATC TGATTTCTAT GGGAGGGAAT CTGAACTTCT AGCCACCAGG 
CAAGATCTTA AGTCTGTAGA AGAGAAGCTT TCTCTAGCAC AGGAGGACTT GATTTCAAAC 
AGAAATCAAA TTGGAAATCA AAATAAATTG ATTCAAGAAC TGAAGACTGC CAAGGCTACA 
TTGGAGCAGG ATTCAGCAAA GAAAGAACAG CAATTGCAGG AGCGATGTAA AGCACTACAA 
GACATTCAGA AAGAAAAGTC ACTGAAAGAA AAAGAACTGG TAAATGAGAA GTCTAAATTG 
GCAGAGATAG AAGAAATTAA ATGTAGACAA GAAAAAGAAA TCACTAAACT AAACGAAGAA 
CTCAAGTCCC ACAAACTAGA AAGCATAAAG GAGATAACAA ATCTTAAAGA TGCTAAACAG 
CTTCTAATTC AGCAGAAATT AGAACTTCAA GGAAAAGCGG ACTCCCTGAA GGCAGCTGTT 
GAACAGGAGA AGAGAAATCA GCAGATACTA AAAGACCAGG TGAAAAAGGA AGAAGAGGAG 
CTGAAGAAAG AATTTATTGA GAAAGAAGCT AAGTTGCATT CCGAAATAAA AGAAAAGGAA 
GTAGGAATGA AGAAGCATGA AGAAAATGAG GCTAAACTTA CCATGCAGAT TACAGCATTA 
AATGAAAACT TAGGCACTGT GAAGAAGGAG TGGCAATCTA GTCAACGGAG AGTTAGTGAG 
CTTGAGAAAC AAACGGATGA CTTACGGGGT GAAATTGCAG TATTAGAAGC AACGGTTCAG 
AATAATCAAG ATGAAAGGAG AGCACTACTG GAAAGATGTC TTAAAGGAGA AGGTGAAATA 
GAAAAGCTTC AAACCAAAGT ATTAGAATTG CAAAGAAAGC TGGATAATAC AACTGCAGCA 
GTGCAGGAGC TGGGCAGAGA AAACCAATCA CTTCAGATCA AACATACACA AGCGTTGAAT 
AGAAAGTGGG CCGAAGACAA TGAAGTACAA AACTGTATGG CCTGTGGGAA AGGCTTTTCA 
GTAACAGTGA GACGGCATCA CTGCCGACAG TGTGGAAATA TCTTCTGTGC TGAATGTTCA 
GCCAAAAATG CCTTAACTCC TTCCTCCAAG AAGCCTGTTC GTGTCTGTGA TGCATGTTTC 
AATGACTTGC AAGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

An endosomal tether undergoes an entropic collapse to bring vesicles together.
Nature537(7618)107-111(2016 09)
Murray DH,Jahnel M,Lauer J,Avellaneda MJ,Brouilly N,Cezanne A,Morales-Navarrete H,Perini ED,Ferguson C,Lupas AN,Kalaidzidis Y,Parton RG,Grill SW,Zerial M

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EEA1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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