EEA1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	EEA1
Entrez Gene ID	516259
Full Name	early endosome antigen 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005198218.3	XP_005198275.1	early endosome antigen 1 isoform X1
XM_005206125.3	XP_005206182.1	early endosome antigen 1 isoform X1
XM_024992403.1	XP_024848171.1	early endosome antigen 1 isoform X3
XM_002687214.5	XP_002687260.2	early endosome antigen 1 isoform X2
XM_005206125.4	XP_005206182.1	early endosome antigen 1 isoform X2
XM_002687214.6	XP_002687260.2	early endosome antigen 1 isoform X4
XM_594411.9	XP_594411.6	early endosome antigen 1 isoform X2
XM_024992402.1	XP_024848170.1	early endosome antigen 1 isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Eea1	NP_001101556.1
Canis lupus familiaris (dog)	EEA1	XP_005629490.1
Gallus gallus (chicken)	EEA1	XP_004937680.1
Xenopus tropicalis (tropical clawed frog)	eea1	XP_002935361.1
Homo sapiens (human)	EEA1	NP_003557.2
	EEA1	XP_001104577.1
Mus musculus (house mouse)	Eea1	NP_001001932.1
Pan troglodytes (chimpanzee)	EEA1	XP_522610.2
Bos taurus (cattle)	EEA1	XP_005206182.1
Danio rerio (zebrafish)	LOC562943	XP_003200485.1

Gene Ontology

The following EEA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EEA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa234726	XM_024992402.1 Latest version!	Bos taurus early endosome antigen 1 (EEA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa234727	XM_024992403.1 Latest version!	Bos taurus early endosome antigen 1 (EEA1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa04184	XM_005206125.4 Latest version!	Bos taurus early endosome antigen 1 (EEA1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa04184	XM_005198218.3 Latest version!	Bos taurus early endosome antigen 1 (EEA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa04185	XM_002687214.6 Latest version!	Bos taurus early endosome antigen 1 (EEA1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa04185	XM_594411.9 Latest version!	Bos taurus early endosome antigen 1 (EEA1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
View Old Accession Versions >>
OBa04184	XM_005206125.3	Bos taurus early endosome antigen 1 (EEA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa04185	XM_002687214.5	Bos taurus early endosome antigen 1 (EEA1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EEA1 ( XM_024992402.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024848170.1 Bos taurus early endosome antigen 1 (EEA1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa234726
Clone ID Related Accession (Same CDS sequence)	XM_024992402.1
Accession Version	XM_024992402.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4437bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	early endosome antigen 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTAAGGA GGATTCTACA GAGGACTCCT GGGAGAGTTG GCTCTCAAGG TTCAGATCTA 
GATTCATCAG CAGCTCCTAT AAACACAGTG GATGTCAATA ATGAAAGCTC TTCAGAGGGT 
TTCATCTGTC CGTTATGTAT GAAAAGATGT ATAACTGCTA TTGATTTGTC GGAGCATTAC 
CAGAAAGTGC ACGCTGAAAA TGAGACAAGA GGACAGGAAC TTCTTAATGA CTCCAGTGTT 
ACTGGTTTCA TATGTCCACA GTGTATGAAA TCTCTTGGAT CTGCTGATGA ACTTTTCAAA 
CACTATGAGG CAGTCCATGA TGCTGGTAAT GACTCAAGTC ACGGAGGAGA AGCTTTGGCT 
CTGAAGCGAG ATGATATAAC ACTACTCAGA CAAGAGGTCC AAGACCTACA GGCTTCACTT 
AAGGAAGAAA AATGGTACTC AGAAGAATTA AAGAAAGAAT TAGAAAAATT TCAAGGGCTG 
CAGCAACAAG AGTCCAAACC TGATGGATTA GTGGCTGATT CATCAGCAGA ACTACAGTCT 
TTGGAACAAC AATTAGAAGA AGCCCAAACA GAAAATTTTA ATATTAAACA AATGAAAGAT 
TTGTTTGAAC AGAAAGCAGC CCAACTTGCT ACTGAAATTG CAGATATAAA GTCAAAATAT 
GATGAAGAAA GGAGTCTTAG AGAAGCTGCT GAACAGCAAG TGACACATCT AACAGAAGAA 
TTAAACAAAG AAGCAGCTGT GATTCAAGAT CTAAAGACTG AACTGCTTCA GAGACCTGGA 
ATAGAAGATG TTGCTGTATT AAAGAAAGAA CTGGTCCAAG TTCAAACACT AATGGATAAC 
ATGACCTTGG AACGTGAGAG AGAATCTGAA AAACTCAAAG ATGAGTGCAA AAAGTTACAA 
ACAGAATATA CTAACTCAGA GGCCGTAATA AGCCAGCTGA GGAGTGAACT TGCCAAAGGC 
CCGCAGGAAG TTGCTGTGTA TGTGCAGGAA CTACAAAAAC TTAAAAGTTC AGTTAATGAA 
TTAACACAAA AAAATCAGAA CTTGACTGAA AAGTTACAGA AGAAAGAACT GGACTATACT 
CAGTTAGAAG AGAAACATAA TGAAGAATGT ATGAGTAAAA AGAGTATACA AGCAAGCCTT 
CATCAAAAAG ACCTGGATTG TCAACAGCTT CAGTCAAGGT TATCTGCATC TGAAACCTCA 
CTGCAGAGAA TACAGGCAGA ACTAGGTGAA AAGGGAGAAG CTACTCAGAA GCTCAAAGAA 
GAATTATCAG AAGTGGAGAC CAAGTACCAG CATCTTAAGG CAGAGTTTAA ACAGCTACAA 
CAACAGAGAG AAGAAAAGGA TCAGCATGGG TTACAACTGC AAAGTGAAAT CAATCAAGTG 
GATTCTTTAC CAGCTGAGCT ACCAGGGAAG CCCATAGTCC ATTCTCCACC CAGGAGCCAG 
AGACATTGTT TTAAATTACA TAGCAAACTT CTGGAGACAG AGCGCCAACT AGGAGAAGCT 
CATGGAAGGC TGAAGGAACA GAGACAGCTT TCAAGTGAAA AGTTGATGGA TAAAGAACAA 
CAAGTGGCTG ATCTACAACT CAAACTTTCT CGTTTAGAGG AAGAGTTGAA GGAAAAAGTA 
ACCAATTCCA CAGAATTGCA GCATCAGTTA GATAAAACAA AGCAACAGCA TCAAGAACAA 
CAAGCTCTTC AACAAAGCAC TACAGCAAAA CTTCGAGAAG CTCAGAATGA TTTGGAACAA 
GTACTACGTC AAATTGGTGA TAAGGACCAA AAGATCCAGA ACCTTGAAGC CTTATTACAG 
AAGAGCAAAG AAAATATTTC TTTACTAGAA AAGGAGAGAG AAGATCTTTA TGCAAAAATT 
CAGGCTGGTG AGGGAGAGAC TGCTGTTCTT AACCAATTAC AAGAAAAAAA CCATACATTA 
CAGGAACAAG TAACTCAGCT AACAGAGAAG CTGAAGAATC AGTCAGAAAG CCATAAACAA 
GCCCAGGAGA ATTTGCATGA CCAGGTGCAG GAGCAGAAGG CACATCTTAG AGCTGCACAA 
GACCGTGTCC TTTCCCTAGA ATCCAGCATC AATGAATTAA ATAGTCAGTT AAATGAAAGC 
AAGGAGAAGG TTTCCCAGCT TGATATACAG GTTAAAGCCA AAACTGAATT ATTGCTATCA 
GCAGAAGCAG CAAAAACTGC TCAGAGAGCA GATCTTCAGA ATCATCTGGA CACAGCCCAA 
AATGCATTAC AAGATAAACA GCAGGAGTTA AATAAGATCA CTACTCAGTT GGATCAGGTC 
ACTACAAAGT TGCAGGACAA GCAAGAACAT TGCAGTCAGC TGGAAAGTCA TCTTAAAGAA 
TACAAAGAGA AACATCTGTC TTTAGAACAG AAAACTGAAG AATTAGAAGG TCAAATCAAG 
AAACTAGAAG CTGATATGCT TGAAGTTAAA GCAAGCAAGG AGCAAGCTTT GCAGGGTCTA 
CAACAACAGA GACAGCTGAA CACAGATTTA GAGCTTCGAG CCACAGAATT GAGTAAACAA 
CTTGAAATGG AGAAAGAAAC AGTATCTAAT ACAAAGTTGG ATCTACAGAA GAAATCTGAA 
GCACTTGAAA ATACTAAGCA AATGCTTACC AAGCAAGAAG AAGAAAAAAC AATGCTTAAA 
CAAGAAATTG AAAATTTAAG TCAAGATGCA AAGATGCAGC ACAAGGAACT GAATGATAGG 
ATTCAAACAG CAGTAACAGA ACTACAGAAA GTGAAGGTGG AGAAAGACTC TCTGGTAGCA 
GAGCTTTCTG CAGCAAAAGA GAAACTGTCA AAAGTTTCTG ATTGTTTGAA GAACTCCCAA 
AGTGAATTTG AAAAAGAAAA TCAGAAGGGA AAAGCTGTTA TATTAGATTT GGAGAAAACT 
TGCAAAGAAT TAAAGCATCA GCTTCAAGTA CAGACAGAAA GCCTGCATAA GGAGCAGAAT 
GAAATGAAAA AGTCACTTGA AAAAGAAAAG GAGACTTCTC ATCAGTTGAA GTTGGAGCTC 
AGTTCCATGC AGGGACAAGT CATACAGGCC CAGGACAGCT TAAAACAAAA GGAAAAAGAA 
GAACAACAGC TTCAGAGTAA CATAAATGAG CTAAAGCAAT TGACTGAACA GAAGAAAAAG 
CAAATTGAAA CACTCCAAGG AGAAGTTAAA ATTGCTGTTT CACAGAAGAC AGAACTTGAG 
AATAAACTAC AGCAGCAGTC AATGCAAGCA GCACAGGAAC TTGCAGCAGA GAAACAAAAA 
ATATCAGTGT TACAAAACAC CTATGAAAAA AGTCAGGAAA ATCTAAAACA GCTTCAGTCG 
GATTTCTATG GGAAGGAATC TGAACTACTT GCCACAAGGC AGGATCTTAA GTCTATAGAA 
GAGAAGCTTT CTCTAGCACA GGAGGATTTG ATATCAAACA GAAATCAAAT TGGAAACCAA 
AATAAATTGA TTCAAGAACT GAAGACCACC AAGACTACTT TGGAGCAAGA TTTAGCAAAG 
AAAGAACAGC AATTGAAGGA ACAGAATAAA GCACTACAAG ATATGCAAAA AGAAAAGTCA 
CTAAAAGAAA AAGAACTAGT AAATGAAAAA TCTAAATTGG CAGAGACAGA GGAAATTAAG 
TGTAGACAAG AAAAAGAAAT TGCTAAATTG AGTGAAGAAC TCAAGTCTCA CAAGCAGGAA 
AGCATAAAGG AAATAACAAA TCTCAAGGAT GCCAAACAGC TTTTGATTCA GCAGAAATTA 
GAGCTTCAAG GGAAAGTAGA TTCCCTGAAG GCAACCCTTG AACAGGAGAA GAAAACCCAG 
CAAATGCTAA AAGAGCAGAT GAAAAAGGAA GAAGATGAGC TGAAGAAAGA GTTTATGGAA 
AAGGAAGCTA AACTGCATTC CGAAATAAAA GAAAAAGAAG TAGGAATGAA GAAGCATGAA 
GAAAATGGGG CTAAACTTAC CATGCAGATC ACAGCATTAA ATGAAAACTT GGGCACCATA 
AAGAAAGAGT GGCAGTCCAG TCAACGGAGA GTCAGTGAAC TCGAGAAACA GACGGACGAC 
TTACGGGGTG AAATTGCAGT ACTGGAAGCG ACAGTTCAGA ATAACCAAGA TGAAAGGAGA 
GCACTGCTGG AAAGGTGTCT TAAAGGAGAA GGTGAAATAG AAAAGCTTCA AACCAAAGTA 
CTAGAATTGC AAAGAAAGCT GGATAATACA ACTGCAGCAG TGCAGGAGCT AGGCAGAGAG 
AATCAGTCCC TTCAAATCAA ACATACACAG GCGTTGACTA GAAAGTGGGC TGAAGACAGT 
GAAGTACAAA ACTGTATGGC CTGTGGGAAA GGCTTTTCCG TAACAGTGAG ACGGCATCAC 
TGCAGACAAT GTGGAAATAT CTTCTGTGCT GAGTGTTCAG CCAAAAATGC CTTAACTCCT 
TCTTCCAAGA AGCCTGTTCG TGTCTGTGAT GGATGTTTCA ATGACTTGCA AGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024848170.1
CDS	254..4690
Translation	MLRRILQRTPGRVGSQGSDLDSSAAPINTVDVNNESSSEGFICPLCMKRCITAIDLSEHYQKVHAENETRGQELLNDSSVTGFICPQCMKSLGSADELFKHYEAVHDAGNDSSHGGEALALKRDDITLLRQEVQDLQASLKEEKWYSEELKKELEKFQGLQQQESKPDGLVADSSAELQSLEQQLEEAQTENFNIKQMKDLFEQKAAQLATEIADIKSKYDEERSLREAAEQQVTHLTEELNKEAAVIQDLKTELLQRPGIEDVAVLKKELVQVQTLMDNMTLERERESEKLKDECKKLQTEYTNSEAVISQLRSELAKGPQEVAVYVQELQKLKSSVNELTQKNQNLTEKLQKKELDYTQLEEKHNEECMSKKSIQASLHQKDLDCQQLQSRLSASETSLQRIQAELGEKGEATQKLKEELSEVETKYQHLKAEFKQLQQQREEKDQHGLQLQSEINQVDSLPAELPGKPIVHSPPRSQRHCFKLHSKLLETERQLGEAHGRLKEQRQLSSEKLMDKEQQVADLQLKLSRLEEELKEKVTNSTELQHQLDKTKQQHQEQQALQQSTTAKLREAQNDLEQVLRQIGDKDQKIQNLEALLQKSKENISLLEKEREDLYAKIQAGEGETAVLNQLQEKNHTLQEQVTQLTEKLKNQSESHKQAQENLHDQVQEQKAHLRAAQDRVLSLESSINELNSQLNESKEKVSQLDIQVKAKTELLLSAEAAKTAQRADLQNHLDTAQNALQDKQQELNKITTQLDQVTTKLQDKQEHCSQLESHLKEYKEKHLSLEQKTEELEGQIKKLEADMLEVKASKEQALQGLQQQRQLNTDLELRATELSKQLEMEKETVSNTKLDLQKKSEALENTKQMLTKQEEEKTMLKQEIENLSQDAKMQHKELNDRIQTAVTELQKVKVEKDSLVAELSAAKEKLSKVSDCLKNSQSEFEKENQKGKAVILDLEKTCKELKHQLQVQTESLHKEQNEMKKSLEKEKETSHQLKLELSSMQGQVIQAQDSLKQKEKEEQQLQSNINELKQLTEQKKKQIETLQGEVKIAVSQKTELENKLQQQSMQAAQELAAEKQKISVLQNTYEKSQENLKQLQSDFYGKESELLATRQDLKSIEEKLSLAQEDLISNRNQIGNQNKLIQELKTTKTTLEQDLAKKEQQLKEQNKALQDMQKEKSLKEKELVNEKSKLAETEEIKCRQEKEIAKLSEELKSHKQESIKEITNLKDAKQLLIQQKLELQGKVDSLKATLEQEKKTQQMLKEQMKKEEDELKKEFMEKEAKLHSEIKEKEVGMKKHEENGAKLTMQITALNENLGTIKKEWQSSQRRVSELEKQTDDLRGEIAVLEATVQNNQDERRALLERCLKGEGEIEKLQTKVLELQRKLDNTTAAVQELGRENQSLQIKHTQALTRKWAEDSEVQNCMACGKGFSVTVRRHHCRQCGNIFCAECSAKNALTPSSKKPVRVCDGCFNDLQG

Target ORF information:

RefSeq Version	XM_024992402.1
Organism	Bos taurus(cattle)
Definition	Bos taurus early endosome antigen 1 (EEA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024992402.1

ORF Insert Sequence:

ATGTTAAGGA GGATTCTACA GAGGACTCCT GGGAGAGTTG GCTCTCAAGG TTCAGATCTA 
GATTCATCAG CAGCTCCTAT AAACACAGTG GATGTCAATA ATGAAAGCTC TTCAGAGGGT 
TTCATCTGTC CGTTATGTAT GAAAAGATGT ATAACTGCTA TTGATTTGTC GGAGCATTAC 
CAGAAAGTGC ACGCTGAAAA TGAGACAAGA GGACAGGAAC TTCTTAATGA CTCCAGTGTT 
ACTGGTTTCA TATGTCCACA GTGTATGAAA TCTCTTGGAT CTGCTGATGA ACTTTTCAAA 
CACTATGAGG CAGTCCATGA TGCTGGTAAT GACTCAAGTC ACGGAGGAGA AGCTTTGGCT 
CTGAAGCGAG ATGATATAAC ACTACTCAGA CAAGAGGTCC AAGACCTACA GGCTTCACTT 
AAGGAAGAAA AATGGTACTC AGAAGAATTA AAGAAAGAAT TAGAAAAATT TCAAGGGCTG 
CAGCAACAAG AGTCCAAACC TGATGGATTA GTGGCTGATT CATCAGCAGA ACTACAGTCT 
TTGGAACAAC AATTAGAAGA AGCCCAAACA GAAAATTTTA ATATTAAACA AATGAAAGAT 
TTGTTTGAAC AGAAAGCAGC CCAACTTGCT ACTGAAATTG CAGATATAAA GTCAAAATAT 
GATGAAGAAA GGAGTCTTAG AGAAGCTGCT GAACAGCAAG TGACACATCT AACAGAAGAA 
TTAAACAAAG AAGCAGCTGT GATTCAAGAT CTAAAGACTG AACTGCTTCA GAGACCTGGA 
ATAGAAGATG TTGCTGTATT AAAGAAAGAA CTGGTCCAAG TTCAAACACT AATGGATAAC 
ATGACCTTGG AACGTGAGAG AGAATCTGAA AAACTCAAAG ATGAGTGCAA AAAGTTACAA 
ACAGAATATA CTAACTCAGA GGCCGTAATA AGCCAGCTGA GGAGTGAACT TGCCAAAGGC 
CCGCAGGAAG TTGCTGTGTA TGTGCAGGAA CTACAAAAAC TTAAAAGTTC AGTTAATGAA 
TTAACACAAA AAAATCAGAA CTTGACTGAA AAGTTACAGA AGAAAGAACT GGACTATACT 
CAGTTAGAAG AGAAACATAA TGAAGAATGT ATGAGTAAAA AGAGTATACA AGCAAGCCTT 
CATCAAAAAG ACCTGGATTG TCAACAGCTT CAGTCAAGGT TATCTGCATC TGAAACCTCA 
CTGCAGAGAA TACAGGCAGA ACTAGGTGAA AAGGGAGAAG CTACTCAGAA GCTCAAAGAA 
GAATTATCAG AAGTGGAGAC CAAGTACCAG CATCTTAAGG CAGAGTTTAA ACAGCTACAA 
CAACAGAGAG AAGAAAAGGA TCAGCATGGG TTACAACTGC AAAGTGAAAT CAATCAAGTG 
GATTCTTTAC CAGCTGAGCT ACCAGGGAAG CCCATAGTCC ATTCTCCACC CAGGAGCCAG 
AGACATTGTT TTAAATTACA TAGCAAACTT CTGGAGACAG AGCGCCAACT AGGAGAAGCT 
CATGGAAGGC TGAAGGAACA GAGACAGCTT TCAAGTGAAA AGTTGATGGA TAAAGAACAA 
CAAGTGGCTG ATCTACAACT CAAACTTTCT CGTTTAGAGG AAGAGTTGAA GGAAAAAGTA 
ACCAATTCCA CAGAATTGCA GCATCAGTTA GATAAAACAA AGCAACAGCA TCAAGAACAA 
CAAGCTCTTC AACAAAGCAC TACAGCAAAA CTTCGAGAAG CTCAGAATGA TTTGGAACAA 
GTACTACGTC AAATTGGTGA TAAGGACCAA AAGATCCAGA ACCTTGAAGC CTTATTACAG 
AAGAGCAAAG AAAATATTTC TTTACTAGAA AAGGAGAGAG AAGATCTTTA TGCAAAAATT 
CAGGCTGGTG AGGGAGAGAC TGCTGTTCTT AACCAATTAC AAGAAAAAAA CCATACATTA 
CAGGAACAAG TAACTCAGCT AACAGAGAAG CTGAAGAATC AGTCAGAAAG CCATAAACAA 
GCCCAGGAGA ATTTGCATGA CCAGGTGCAG GAGCAGAAGG CACATCTTAG AGCTGCACAA 
GACCGTGTCC TTTCCCTAGA ATCCAGCATC AATGAATTAA ATAGTCAGTT AAATGAAAGC 
AAGGAGAAGG TTTCCCAGCT TGATATACAG GTTAAAGCCA AAACTGAATT ATTGCTATCA 
GCAGAAGCAG CAAAAACTGC TCAGAGAGCA GATCTTCAGA ATCATCTGGA CACAGCCCAA 
AATGCATTAC AAGATAAACA GCAGGAGTTA AATAAGATCA CTACTCAGTT GGATCAGGTC 
ACTACAAAGT TGCAGGACAA GCAAGAACAT TGCAGTCAGC TGGAAAGTCA TCTTAAAGAA 
TACAAAGAGA AACATCTGTC TTTAGAACAG AAAACTGAAG AATTAGAAGG TCAAATCAAG 
AAACTAGAAG CTGATATGCT TGAAGTTAAA GCAAGCAAGG AGCAAGCTTT GCAGGGTCTA 
CAACAACAGA GACAGCTGAA CACAGATTTA GAGCTTCGAG CCACAGAATT GAGTAAACAA 
CTTGAAATGG AGAAAGAAAC AGTATCTAAT ACAAAGTTGG ATCTACAGAA GAAATCTGAA 
GCACTTGAAA ATACTAAGCA AATGCTTACC AAGCAAGAAG AAGAAAAAAC AATGCTTAAA 
CAAGAAATTG AAAATTTAAG TCAAGATGCA AAGATGCAGC ACAAGGAACT GAATGATAGG 
ATTCAAACAG CAGTAACAGA ACTACAGAAA GTGAAGGTGG AGAAAGACTC TCTGGTAGCA 
GAGCTTTCTG CAGCAAAAGA GAAACTGTCA AAAGTTTCTG ATTGTTTGAA GAACTCCCAA 
AGTGAATTTG AAAAAGAAAA TCAGAAGGGA AAAGCTGTTA TATTAGATTT GGAGAAAACT 
TGCAAAGAAT TAAAGCATCA GCTTCAAGTA CAGACAGAAA GCCTGCATAA GGAGCAGAAT 
GAAATGAAAA AGTCACTTGA AAAAGAAAAG GAGACTTCTC ATCAGTTGAA GTTGGAGCTC 
AGTTCCATGC AGGGACAAGT CATACAGGCC CAGGACAGCT TAAAACAAAA GGAAAAAGAA 
GAACAACAGC TTCAGAGTAA CATAAATGAG CTAAAGCAAT TGACTGAACA GAAGAAAAAG 
CAAATTGAAA CACTCCAAGG AGAAGTTAAA ATTGCTGTTT CACAGAAGAC AGAACTTGAG 
AATAAACTAC AGCAGCAGTC AATGCAAGCA GCACAGGAAC TTGCAGCAGA GAAACAAAAA 
ATATCAGTGT TACAAAACAC CTATGAAAAA AGTCAGGAAA ATCTAAAACA GCTTCAGTCG 
GATTTCTATG GGAAGGAATC TGAACTACTT GCCACAAGGC AGGATCTTAA GTCTATAGAA 
GAGAAGCTTT CTCTAGCACA GGAGGATTTG ATATCAAACA GAAATCAAAT TGGAAACCAA 
AATAAATTGA TTCAAGAACT GAAGACCACC AAGACTACTT TGGAGCAAGA TTTAGCAAAG 
AAAGAACAGC AATTGAAGGA ACAGAATAAA GCACTACAAG ATATGCAAAA AGAAAAGTCA 
CTAAAAGAAA AAGAACTAGT AAATGAAAAA TCTAAATTGG CAGAGACAGA GGAAATTAAG 
TGTAGACAAG AAAAAGAAAT TGCTAAATTG AGTGAAGAAC TCAAGTCTCA CAAGCAGGAA 
AGCATAAAGG AAATAACAAA TCTCAAGGAT GCCAAACAGC TTTTGATTCA GCAGAAATTA 
GAGCTTCAAG GGAAAGTAGA TTCCCTGAAG GCAACCCTTG AACAGGAGAA GAAAACCCAG 
CAAATGCTAA AAGAGCAGAT GAAAAAGGAA GAAGATGAGC TGAAGAAAGA GTTTATGGAA 
AAGGAAGCTA AACTGCATTC CGAAATAAAA GAAAAAGAAG TAGGAATGAA GAAGCATGAA 
GAAAATGGGG CTAAACTTAC CATGCAGATC ACAGCATTAA ATGAAAACTT GGGCACCATA 
AAGAAAGAGT GGCAGTCCAG TCAACGGAGA GTCAGTGAAC TCGAGAAACA GACGGACGAC 
TTACGGGGTG AAATTGCAGT ACTGGAAGCG ACAGTTCAGA ATAACCAAGA TGAAAGGAGA 
GCACTGCTGG AAAGGTGTCT TAAAGGAGAA GGTGAAATAG AAAAGCTTCA AACCAAAGTA 
CTAGAATTGC AAAGAAAGCT GGATAATACA ACTGCAGCAG TGCAGGAGCT AGGCAGAGAG 
AATCAGTCCC TTCAAATCAA ACATACACAG GCGTTGACTA GAAAGTGGGC TGAAGACAGT 
GAAGTACAAA ACTGTATGGC CTGTGGGAAA GGCTTTTCCG TAACAGTGAG ACGGCATCAC 
TGCAGACAAT GTGGAAATAT CTTCTGTGCT GAGTGTTCAG CCAAAAATGC CTTAACTCCT 
TCTTCCAAGA AGCCTGTTCG TGTCTGTGAT GGATGTTTCA ATGACTTGCA AGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EEA1 ( XM_024992403.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024848171.1 Bos taurus early endosome antigen 1 (EEA1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa234727
Clone ID Related Accession (Same CDS sequence)	XM_024992403.1
Accession Version	XM_024992403.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4311bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	early endosome antigen 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTAAGGA GGATTCTACA GAGGACTCCT GGGAGAGTTG GCTCTCAAGG TTCAGATCTA 
GATTCATCAG CAGCTCCTAT AAACACAGTG GATGTCAATA ATGAAAGCTC TTCAGAGGGT 
TTCATATGTC CACAGTGTAT GAAATCTCTT GGATCTGCTG ATGAACTTTT CAAACACTAT 
GAGGCAGTCC ATGATGCTGG TAATGACTCA AGTCACGGAG GAGAAGCTTT GGCTCTGAAG 
CGAGATGATA TAACACTACT CAGACAAGAG GTCCAAGACC TACAGGCTTC ACTTAAGGAA 
GAAAAATGGT ACTCAGAAGA ATTAAAGAAA GAATTAGAAA AATTTCAAGG GCTGCAGCAA 
CAAGAGTCCA AACCTGATGG ATTAGTGGCT GATTCATCAG CAGAACTACA GTCTTTGGAA 
CAACAATTAG AAGAAGCCCA AACAGAAAAT TTTAATATTA AACAAATGAA AGATTTGTTT 
GAACAGAAAG CAGCCCAACT TGCTACTGAA ATTGCAGATA TAAAGTCAAA ATATGATGAA 
GAAAGGAGTC TTAGAGAAGC TGCTGAACAG CAAGTGACAC ATCTAACAGA AGAATTAAAC 
AAAGAAGCAG CTGTGATTCA AGATCTAAAG ACTGAACTGC TTCAGAGACC TGGAATAGAA 
GATGTTGCTG TATTAAAGAA AGAACTGGTC CAAGTTCAAA CACTAATGGA TAACATGACC 
TTGGAACGTG AGAGAGAATC TGAAAAACTC AAAGATGAGT GCAAAAAGTT ACAAACAGAA 
TATACTAACT CAGAGGCCGT AATAAGCCAG CTGAGGAGTG AACTTGCCAA AGGCCCGCAG 
GAAGTTGCTG TGTATGTGCA GGAACTACAA AAACTTAAAA GTTCAGTTAA TGAATTAACA 
CAAAAAAATC AGAACTTGAC TGAAAAGTTA CAGAAGAAAG AACTGGACTA TACTCAGTTA 
GAAGAGAAAC ATAATGAAGA ATGTATGAGT AAAAAGAGTA TACAAGCAAG CCTTCATCAA 
AAAGACCTGG ATTGTCAACA GCTTCAGTCA AGGTTATCTG CATCTGAAAC CTCACTGCAG 
AGAATACAGG CAGAACTAGG TGAAAAGGGA GAAGCTACTC AGAAGCTCAA AGAAGAATTA 
TCAGAAGTGG AGACCAAGTA CCAGCATCTT AAGGCAGAGT TTAAACAGCT ACAACAACAG 
AGAGAAGAAA AGGATCAGCA TGGGTTACAA CTGCAAAGTG AAATCAATCA AGTGGATTCT 
TTACCAGCTG AGCTACCAGG GAAGCCCATA GTCCATTCTC CACCCAGGAG CCAGAGACAT 
TGTTTTAAAT TACATAGCAA ACTTCTGGAG ACAGAGCGCC AACTAGGAGA AGCTCATGGA 
AGGCTGAAGG AACAGAGACA GCTTTCAAGT GAAAAGTTGA TGGATAAAGA ACAACAAGTG 
GCTGATCTAC AACTCAAACT TTCTCGTTTA GAGGAAGAGT TGAAGGAAAA AGTAACCAAT 
TCCACAGAAT TGCAGCATCA GTTAGATAAA ACAAAGCAAC AGCATCAAGA ACAACAAGCT 
CTTCAACAAA GCACTACAGC AAAACTTCGA GAAGCTCAGA ATGATTTGGA ACAAGTACTA 
CGTCAAATTG GTGATAAGGA CCAAAAGATC CAGAACCTTG AAGCCTTATT ACAGAAGAGC 
AAAGAAAATA TTTCTTTACT AGAAAAGGAG AGAGAAGATC TTTATGCAAA AATTCAGGCT 
GGTGAGGGAG AGACTGCTGT TCTTAACCAA TTACAAGAAA AAAACCATAC ATTACAGGAA 
CAAGTAACTC AGCTAACAGA GAAGCTGAAG AATCAGTCAG AAAGCCATAA ACAAGCCCAG 
GAGAATTTGC ATGACCAGGT GCAGGAGCAG AAGGCACATC TTAGAGCTGC ACAAGACCGT 
GTCCTTTCCC TAGAATCCAG CATCAATGAA TTAAATAGTC AGTTAAATGA AAGCAAGGAG 
AAGGTTTCCC AGCTTGATAT ACAGGTTAAA GCCAAAACTG AATTATTGCT ATCAGCAGAA 
GCAGCAAAAA CTGCTCAGAG AGCAGATCTT CAGAATCATC TGGACACAGC CCAAAATGCA 
TTACAAGATA AACAGCAGGA GTTAAATAAG ATCACTACTC AGTTGGATCA GGTCACTACA 
AAGTTGCAGG ACAAGCAAGA ACATTGCAGT CAGCTGGAAA GTCATCTTAA AGAATACAAA 
GAGAAACATC TGTCTTTAGA ACAGAAAACT GAAGAATTAG AAGGTCAAAT CAAGAAACTA 
GAAGCTGATA TGCTTGAAGT TAAAGCAAGC AAGGAGCAAG CTTTGCAGGG TCTACAACAA 
CAGAGACAGC TGAACACAGA TTTAGAGCTT CGAGCCACAG AATTGAGTAA ACAACTTGAA 
ATGGAGAAAG AAACAGTATC TAATACAAAG TTGGATCTAC AGAAGAAATC TGAAGCACTT 
GAAAATACTA AGCAAATGCT TACCAAGCAA GAAGAAGAAA AAACAATGCT TAAACAAGAA 
ATTGAAAATT TAAGTCAAGA TGCAAAGATG CAGCACAAGG AACTGAATGA TAGGATTCAA 
ACAGCAGTAA CAGAACTACA GAAAGTGAAG GTGGAGAAAG ACTCTCTGGT AGCAGAGCTT 
TCTGCAGCAA AAGAGAAACT GTCAAAAGTT TCTGATTGTT TGAAGAACTC CCAAAGTGAA 
TTTGAAAAAG AAAATCAGAA GGGAAAAGCT GTTATATTAG ATTTGGAGAA AACTTGCAAA 
GAATTAAAGC ATCAGCTTCA AGTACAGACA GAAAGCCTGC ATAAGGAGCA GAATGAAATG 
AAAAAGTCAC TTGAAAAAGA AAAGGAGACT TCTCATCAGT TGAAGTTGGA GCTCAGTTCC 
ATGCAGGGAC AAGTCATACA GGCCCAGGAC AGCTTAAAAC AAAAGGAAAA AGAAGAACAA 
CAGCTTCAGA GTAACATAAA TGAGCTAAAG CAATTGACTG AACAGAAGAA AAAGCAAATT 
GAAACACTCC AAGGAGAAGT TAAAATTGCT GTTTCACAGA AGACAGAACT TGAGAATAAA 
CTACAGCAGC AGTCAATGCA AGCAGCACAG GAACTTGCAG CAGAGAAACA AAAAATATCA 
GTGTTACAAA ACACCTATGA AAAAAGTCAG GAAAATCTAA AACAGCTTCA GTCGGATTTC 
TATGGGAAGG AATCTGAACT ACTTGCCACA AGGCAGGATC TTAAGTCTAT AGAAGAGAAG 
CTTTCTCTAG CACAGGAGGA TTTGATATCA AACAGAAATC AAATTGGAAA CCAAAATAAA 
TTGATTCAAG AACTGAAGAC CACCAAGACT ACTTTGGAGC AAGATTTAGC AAAGAAAGAA 
CAGCAATTGA AGGAACAGAA TAAAGCACTA CAAGATATGC AAAAAGAAAA GTCACTAAAA 
GAAAAAGAAC TAGTAAATGA AAAATCTAAA TTGGCAGAGA CAGAGGAAAT TAAGTGTAGA 
CAAGAAAAAG AAATTGCTAA ATTGAGTGAA GAACTCAAGT CTCACAAGCA GGAAAGCATA 
AAGGAAATAA CAAATCTCAA GGATGCCAAA CAGCTTTTGA TTCAGCAGAA ATTAGAGCTT 
CAAGGGAAAG TAGATTCCCT GAAGGCAACC CTTGAACAGG AGAAGAAAAC CCAGCAAATG 
CTAAAAGAGC AGATGAAAAA GGAAGAAGAT GAGCTGAAGA AAGAGTTTAT GGAAAAGGAA 
GCTAAACTGC ATTCCGAAAT AAAAGAAAAA GAAGTAGGAA TGAAGAAGCA TGAAGAAAAT 
GGGGCTAAAC TTACCATGCA GATCACAGCA TTAAATGAAA ACTTGGGCAC CATAAAGAAA 
GAGTGGCAGT CCAGTCAACG GAGAGTCAGT GAACTCGAGA AACAGACGGA CGACTTACGG 
GGTGAAATTG CAGTACTGGA AGCGACAGTT CAGAATAACC AAGATGAAAG GAGAGCACTG 
CTGGAAAGGT GTCTTAAAGG AGAAGGTGAA ATAGAAAAGC TTCAAACCAA AGTACTAGAA 
TTGCAAAGAA AGCTGGATAA TACAACTGCA GCAGTGCAGG AGCTAGGCAG AGAGAATCAG 
TCCCTTCAAA TCAAACATAC ACAGGCGTTG ACTAGAAAGT GGGCTGAAGA CAGTGAAGTA 
CAAAACTGTA TGGCCTGTGG GAAAGGCTTT TCCGTAACAG TGAGACGGCA TCACTGCAGA 
CAATGTGGAA ATATCTTCTG TGCTGAGTGT TCAGCCAAAA ATGCCTTAAC TCCTTCTTCC 
AAGAAGCCTG TTCGTGTCTG TGATGGATGT TTCAATGACT TGCAAGGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024848171.1
CDS	254..4564
Translation	MLRRILQRTPGRVGSQGSDLDSSAAPINTVDVNNESSSEGFICPQCMKSLGSADELFKHYEAVHDAGNDSSHGGEALALKRDDITLLRQEVQDLQASLKEEKWYSEELKKELEKFQGLQQQESKPDGLVADSSAELQSLEQQLEEAQTENFNIKQMKDLFEQKAAQLATEIADIKSKYDEERSLREAAEQQVTHLTEELNKEAAVIQDLKTELLQRPGIEDVAVLKKELVQVQTLMDNMTLERERESEKLKDECKKLQTEYTNSEAVISQLRSELAKGPQEVAVYVQELQKLKSSVNELTQKNQNLTEKLQKKELDYTQLEEKHNEECMSKKSIQASLHQKDLDCQQLQSRLSASETSLQRIQAELGEKGEATQKLKEELSEVETKYQHLKAEFKQLQQQREEKDQHGLQLQSEINQVDSLPAELPGKPIVHSPPRSQRHCFKLHSKLLETERQLGEAHGRLKEQRQLSSEKLMDKEQQVADLQLKLSRLEEELKEKVTNSTELQHQLDKTKQQHQEQQALQQSTTAKLREAQNDLEQVLRQIGDKDQKIQNLEALLQKSKENISLLEKEREDLYAKIQAGEGETAVLNQLQEKNHTLQEQVTQLTEKLKNQSESHKQAQENLHDQVQEQKAHLRAAQDRVLSLESSINELNSQLNESKEKVSQLDIQVKAKTELLLSAEAAKTAQRADLQNHLDTAQNALQDKQQELNKITTQLDQVTTKLQDKQEHCSQLESHLKEYKEKHLSLEQKTEELEGQIKKLEADMLEVKASKEQALQGLQQQRQLNTDLELRATELSKQLEMEKETVSNTKLDLQKKSEALENTKQMLTKQEEEKTMLKQEIENLSQDAKMQHKELNDRIQTAVTELQKVKVEKDSLVAELSAAKEKLSKVSDCLKNSQSEFEKENQKGKAVILDLEKTCKELKHQLQVQTESLHKEQNEMKKSLEKEKETSHQLKLELSSMQGQVIQAQDSLKQKEKEEQQLQSNINELKQLTEQKKKQIETLQGEVKIAVSQKTELENKLQQQSMQAAQELAAEKQKISVLQNTYEKSQENLKQLQSDFYGKESELLATRQDLKSIEEKLSLAQEDLISNRNQIGNQNKLIQELKTTKTTLEQDLAKKEQQLKEQNKALQDMQKEKSLKEKELVNEKSKLAETEEIKCRQEKEIAKLSEELKSHKQESIKEITNLKDAKQLLIQQKLELQGKVDSLKATLEQEKKTQQMLKEQMKKEEDELKKEFMEKEAKLHSEIKEKEVGMKKHEENGAKLTMQITALNENLGTIKKEWQSSQRRVSELEKQTDDLRGEIAVLEATVQNNQDERRALLERCLKGEGEIEKLQTKVLELQRKLDNTTAAVQELGRENQSLQIKHTQALTRKWAEDSEVQNCMACGKGFSVTVRRHHCRQCGNIFCAECSAKNALTPSSKKPVRVCDGCFNDLQG

Target ORF information:

RefSeq Version	XM_024992403.1
Organism	Bos taurus(cattle)
Definition	Bos taurus early endosome antigen 1 (EEA1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024992403.1

ORF Insert Sequence:

ATGTTAAGGA GGATTCTACA GAGGACTCCT GGGAGAGTTG GCTCTCAAGG TTCAGATCTA 
GATTCATCAG CAGCTCCTAT AAACACAGTG GATGTCAATA ATGAAAGCTC TTCAGAGGGT 
TTCATATGTC CACAGTGTAT GAAATCTCTT GGATCTGCTG ATGAACTTTT CAAACACTAT 
GAGGCAGTCC ATGATGCTGG TAATGACTCA AGTCACGGAG GAGAAGCTTT GGCTCTGAAG 
CGAGATGATA TAACACTACT CAGACAAGAG GTCCAAGACC TACAGGCTTC ACTTAAGGAA 
GAAAAATGGT ACTCAGAAGA ATTAAAGAAA GAATTAGAAA AATTTCAAGG GCTGCAGCAA 
CAAGAGTCCA AACCTGATGG ATTAGTGGCT GATTCATCAG CAGAACTACA GTCTTTGGAA 
CAACAATTAG AAGAAGCCCA AACAGAAAAT TTTAATATTA AACAAATGAA AGATTTGTTT 
GAACAGAAAG CAGCCCAACT TGCTACTGAA ATTGCAGATA TAAAGTCAAA ATATGATGAA 
GAAAGGAGTC TTAGAGAAGC TGCTGAACAG CAAGTGACAC ATCTAACAGA AGAATTAAAC 
AAAGAAGCAG CTGTGATTCA AGATCTAAAG ACTGAACTGC TTCAGAGACC TGGAATAGAA 
GATGTTGCTG TATTAAAGAA AGAACTGGTC CAAGTTCAAA CACTAATGGA TAACATGACC 
TTGGAACGTG AGAGAGAATC TGAAAAACTC AAAGATGAGT GCAAAAAGTT ACAAACAGAA 
TATACTAACT CAGAGGCCGT AATAAGCCAG CTGAGGAGTG AACTTGCCAA AGGCCCGCAG 
GAAGTTGCTG TGTATGTGCA GGAACTACAA AAACTTAAAA GTTCAGTTAA TGAATTAACA 
CAAAAAAATC AGAACTTGAC TGAAAAGTTA CAGAAGAAAG AACTGGACTA TACTCAGTTA 
GAAGAGAAAC ATAATGAAGA ATGTATGAGT AAAAAGAGTA TACAAGCAAG CCTTCATCAA 
AAAGACCTGG ATTGTCAACA GCTTCAGTCA AGGTTATCTG CATCTGAAAC CTCACTGCAG 
AGAATACAGG CAGAACTAGG TGAAAAGGGA GAAGCTACTC AGAAGCTCAA AGAAGAATTA 
TCAGAAGTGG AGACCAAGTA CCAGCATCTT AAGGCAGAGT TTAAACAGCT ACAACAACAG 
AGAGAAGAAA AGGATCAGCA TGGGTTACAA CTGCAAAGTG AAATCAATCA AGTGGATTCT 
TTACCAGCTG AGCTACCAGG GAAGCCCATA GTCCATTCTC CACCCAGGAG CCAGAGACAT 
TGTTTTAAAT TACATAGCAA ACTTCTGGAG ACAGAGCGCC AACTAGGAGA AGCTCATGGA 
AGGCTGAAGG AACAGAGACA GCTTTCAAGT GAAAAGTTGA TGGATAAAGA ACAACAAGTG 
GCTGATCTAC AACTCAAACT TTCTCGTTTA GAGGAAGAGT TGAAGGAAAA AGTAACCAAT 
TCCACAGAAT TGCAGCATCA GTTAGATAAA ACAAAGCAAC AGCATCAAGA ACAACAAGCT 
CTTCAACAAA GCACTACAGC AAAACTTCGA GAAGCTCAGA ATGATTTGGA ACAAGTACTA 
CGTCAAATTG GTGATAAGGA CCAAAAGATC CAGAACCTTG AAGCCTTATT ACAGAAGAGC 
AAAGAAAATA TTTCTTTACT AGAAAAGGAG AGAGAAGATC TTTATGCAAA AATTCAGGCT 
GGTGAGGGAG AGACTGCTGT TCTTAACCAA TTACAAGAAA AAAACCATAC ATTACAGGAA 
CAAGTAACTC AGCTAACAGA GAAGCTGAAG AATCAGTCAG AAAGCCATAA ACAAGCCCAG 
GAGAATTTGC ATGACCAGGT GCAGGAGCAG AAGGCACATC TTAGAGCTGC ACAAGACCGT 
GTCCTTTCCC TAGAATCCAG CATCAATGAA TTAAATAGTC AGTTAAATGA AAGCAAGGAG 
AAGGTTTCCC AGCTTGATAT ACAGGTTAAA GCCAAAACTG AATTATTGCT ATCAGCAGAA 
GCAGCAAAAA CTGCTCAGAG AGCAGATCTT CAGAATCATC TGGACACAGC CCAAAATGCA 
TTACAAGATA AACAGCAGGA GTTAAATAAG ATCACTACTC AGTTGGATCA GGTCACTACA 
AAGTTGCAGG ACAAGCAAGA ACATTGCAGT CAGCTGGAAA GTCATCTTAA AGAATACAAA 
GAGAAACATC TGTCTTTAGA ACAGAAAACT GAAGAATTAG AAGGTCAAAT CAAGAAACTA 
GAAGCTGATA TGCTTGAAGT TAAAGCAAGC AAGGAGCAAG CTTTGCAGGG TCTACAACAA 
CAGAGACAGC TGAACACAGA TTTAGAGCTT CGAGCCACAG AATTGAGTAA ACAACTTGAA 
ATGGAGAAAG AAACAGTATC TAATACAAAG TTGGATCTAC AGAAGAAATC TGAAGCACTT 
GAAAATACTA AGCAAATGCT TACCAAGCAA GAAGAAGAAA AAACAATGCT TAAACAAGAA 
ATTGAAAATT TAAGTCAAGA TGCAAAGATG CAGCACAAGG AACTGAATGA TAGGATTCAA 
ACAGCAGTAA CAGAACTACA GAAAGTGAAG GTGGAGAAAG ACTCTCTGGT AGCAGAGCTT 
TCTGCAGCAA AAGAGAAACT GTCAAAAGTT TCTGATTGTT TGAAGAACTC CCAAAGTGAA 
TTTGAAAAAG AAAATCAGAA GGGAAAAGCT GTTATATTAG ATTTGGAGAA AACTTGCAAA 
GAATTAAAGC ATCAGCTTCA AGTACAGACA GAAAGCCTGC ATAAGGAGCA GAATGAAATG 
AAAAAGTCAC TTGAAAAAGA AAAGGAGACT TCTCATCAGT TGAAGTTGGA GCTCAGTTCC 
ATGCAGGGAC AAGTCATACA GGCCCAGGAC AGCTTAAAAC AAAAGGAAAA AGAAGAACAA 
CAGCTTCAGA GTAACATAAA TGAGCTAAAG CAATTGACTG AACAGAAGAA AAAGCAAATT 
GAAACACTCC AAGGAGAAGT TAAAATTGCT GTTTCACAGA AGACAGAACT TGAGAATAAA 
CTACAGCAGC AGTCAATGCA AGCAGCACAG GAACTTGCAG CAGAGAAACA AAAAATATCA 
GTGTTACAAA ACACCTATGA AAAAAGTCAG GAAAATCTAA AACAGCTTCA GTCGGATTTC 
TATGGGAAGG AATCTGAACT ACTTGCCACA AGGCAGGATC TTAAGTCTAT AGAAGAGAAG 
CTTTCTCTAG CACAGGAGGA TTTGATATCA AACAGAAATC AAATTGGAAA CCAAAATAAA 
TTGATTCAAG AACTGAAGAC CACCAAGACT ACTTTGGAGC AAGATTTAGC AAAGAAAGAA 
CAGCAATTGA AGGAACAGAA TAAAGCACTA CAAGATATGC AAAAAGAAAA GTCACTAAAA 
GAAAAAGAAC TAGTAAATGA AAAATCTAAA TTGGCAGAGA CAGAGGAAAT TAAGTGTAGA 
CAAGAAAAAG AAATTGCTAA ATTGAGTGAA GAACTCAAGT CTCACAAGCA GGAAAGCATA 
AAGGAAATAA CAAATCTCAA GGATGCCAAA CAGCTTTTGA TTCAGCAGAA ATTAGAGCTT 
CAAGGGAAAG TAGATTCCCT GAAGGCAACC CTTGAACAGG AGAAGAAAAC CCAGCAAATG 
CTAAAAGAGC AGATGAAAAA GGAAGAAGAT GAGCTGAAGA AAGAGTTTAT GGAAAAGGAA 
GCTAAACTGC ATTCCGAAAT AAAAGAAAAA GAAGTAGGAA TGAAGAAGCA TGAAGAAAAT 
GGGGCTAAAC TTACCATGCA GATCACAGCA TTAAATGAAA ACTTGGGCAC CATAAAGAAA 
GAGTGGCAGT CCAGTCAACG GAGAGTCAGT GAACTCGAGA AACAGACGGA CGACTTACGG 
GGTGAAATTG CAGTACTGGA AGCGACAGTT CAGAATAACC AAGATGAAAG GAGAGCACTG 
CTGGAAAGGT GTCTTAAAGG AGAAGGTGAA ATAGAAAAGC TTCAAACCAA AGTACTAGAA 
TTGCAAAGAA AGCTGGATAA TACAACTGCA GCAGTGCAGG AGCTAGGCAG AGAGAATCAG 
TCCCTTCAAA TCAAACATAC ACAGGCGTTG ACTAGAAAGT GGGCTGAAGA CAGTGAAGTA 
CAAAACTGTA TGGCCTGTGG GAAAGGCTTT TCCGTAACAG TGAGACGGCA TCACTGCAGA 
CAATGTGGAA ATATCTTCTG TGCTGAGTGT TCAGCCAAAA ATGCCTTAAC TCCTTCTTCC 
AAGAAGCCTG TTCGTGTCTG TGATGGATGT TTCAATGACT TGCAAGGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EEA1 ( XM_005198218.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005198275.1 Bos taurus early endosome antigen 1 (EEA1), transcript variant X1, mRNA.

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CloneID	OBa04184
Clone ID Related Accession (Same CDS sequence)	XM_005198218.3 , XM_005206125.3 , XM_005206125.4
Accession Version	XM_005198218.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	early endosome antigen 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644784.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005198218.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTAAGGA GGATTCTACA GAGGACTCCT GGGAGAGTTG GCTCTCAAGG TTCAGATCTA 
GATTCATCAG CAGCTCCTAT AAACACAGTG GATGTCAATA ATGAAAGCTC TTCAGAGGGT 
TTCATCTGTC CGTTATGTAT GAAAAGATGT ATAACTGCTA TTGATTTGTC GGAGCATTAC 
CAGAAAGTGC ACGCTGAAAA TGAGACAAGA GGACAGGAAC TTCTTAATGA CTCCAGTGTT 
ACTGGTTTCA TATGTCCACA GTGTATGAAA TCTCTTGGAT CTGCTGATGA ACTTTTCAAA 
CACTATGAGG CAGTCCATGA TGCTGGTAAT GACTCAAGTC ACGGAGGAGA AGCTTTGGCT 
CTGAAGCGAG ATGATATAAC ACTACTCAGA CAAGAGGTCC AAGACCTACA GGCTTCACTT 
AAGGAAGAAA AATGGTACTC AGAAGAATTA AAGAAAGAAT TAGAAAAATT TCAAGGGCTG 
CAGCAACAAG AGTCCAAACC TGATGGATTA GTGGCTGATT CATCAGCAGA ACTACAGTCT 
TTGGAACAAC AATTAGAAGA AGCCCAAACA GAAAATTTTA ATATTAAACA AATGAAAGAT 
TTGTTTGAAC AGAAAGCAGC CCAACTTGCT ACTGAAATTG CAGATATAAA GTCAAAATAT 
GATGAAGAAA GGAGTCTTAG AGAAGCTGCT GAACAGCAAG TGACACATCT AACAGAAGAA 
TTAAACAAAG AAGCAGCTGT GATTCAAGAT CTAAAGACTG AACTGCTTCA GAGACCTGGA 
ATAGAAGATG TTGCTGTATT AAAGAAAGAA CTGGTCCAAG TTCAAACACT AATGGATAAC 
ATGACCTTGG AACGTGAGAG AGAATCTGAA AAACTCAAAG ATGAGTGCAA AAAGTTACAA 
ACAGAATATA CTAACTCAGA GGCCGTAATA AGCCAGCTGA GGAGTGAACT TGCCAAAGGC 
CCGCAGGAAG TTGCTGTGTA TGTGCAGGAA CTACAAAAAC TTAAAAGTTC AGTTAATGAA 
TTAACACAAA AAAATCAGAA CTTGACTGAA AAGTTACAGA AGAAAGAACT GGACTATACT 
CAGTTAGAAG AGAAACATAA TGAAGAATGT ATGAGTAAAA AGAGTATACA AGCAAGCCTT 
CATCAAAAAG ACCTGGATTG TCAACAGCTT CAGTCAAGGT TATCTGCATC TGAAACCTCA 
CTGCAGAGAA TACAGGCAGA ACTAGGTGAA AAGGGAGAAG CTACTCAGAA GCTCAAAGAA 
GAATTATCAG AAGTGGAGAC CAAGTACCAG CATCTTAAGG CAGAGTTTAA ACAGCTACAA 
CAACAGAGAG AAGAAAAGGA TCAGCATGGG TTACAACTGC AAAGTGAAAT CAATCAATTA 
CATAGCAAAC TTCTGGAGAC AGAGCGCCAA CTAGGAGAAG CTCATGGAAG GCTGAAGGAA 
CAGAGACAGC TTTCAAGTGA AAAGTTGATG GATAAAGAAC AACAAGTGGC TGATCTACAA 
CTCAAACTTT CTCGTTTAGA GGAAGAGTTG AAGGAAAAAG TAACCAATTC CACAGAATTG 
CAGCATCAGT TAGATAAAAC AAAGCAACAG CATCAAGAAC AACAAGCTCT TCAACAAAGC 
ACTACAGCAA AACTTCGAGA AGCTCAGAAT GATTTGGAAC AAGTACTACG TCAAATTGGT 
GATAAGGACC AAAAGATCCA GAACCTTGAA GCCTTATTAC AGAAGAGCAA AGAAAATATT 
TCTTTACTAG AAAAGGAGAG AGAAGATCTT TATGCAAAAA TTCAGGCTGG TGAGGGAGAG 
ACTGCTGTTC TTAACCAATT ACAAGAAAAA AACCATACAT TACAGGAACA AGTAACTCAG 
CTAACAGAGA AGCTGAAGAA TCAGTCAGAA AGCCATAAAC AAGCCCAGGA GAATTTGCAT 
GACCAGGTGC AGGAGCAGAA GGCACATCTT AGAGCTGCAC AAGACCGTGT CCTTTCCCTA 
GAATCCAGCA TCAATGAATT AAATAGTCAG TTAAATGAAA GCAAGGAGAA GGTTTCCCAG 
CTTGATATAC AGGTTAAAGC CAAAACTGAA TTATTGCTAT CAGCAGAAGC AGCAAAAACT 
GCTCAGAGAG CAGATCTTCA GAATCATCTG GACACAGCCC AAAATGCATT ACAAGATAAA 
CAGCAGGAGT TAAATAAGAT CACTACTCAG TTGGATCAGG TCACTACAAA GTTGCAGGAC 
AAGCAAGAAC ATTGCAGTCA GCTGGAAAGT CATCTTAAAG AATACAAAGA GAAACATCTG 
TCTTTAGAAC AGAAAACTGA AGAATTAGAA GGTCAAATCA AGAAACTAGA AGCTGATATG 
CTTGAAGTTA AAGCAAGCAA GGAGCAAGCT TTGCAGGGTC TACAACAACA GAGACAGCTG 
AACACAGATT TAGAGCTTCG AGCCACAGAA TTGAGTAAAC AACTTGAAAT GGAGAAAGAA 
ACAGTATCTA ATACAAAGTT GGATCTACAG AAGAAATCTG AAGCACTTGA AAATACTAAG 
CAAATGCTTA CCAAGCAAGA AGAAGAAAAA ACAATGCTTA AACAAGAAAT TGAAAATTTA 
AGTCAAGATG CAAAGATGCA GCACAAGGAA CTGAATGATA GGATTCAAAC AGCAGTAACA 
GAACTACAGA AAGTGAAGGT GGAGAAAGAC TCTCTGGTAG CAGAGCTTTC TGCAGCAAAA 
GAGAAACTGT CAAAAGTTTC TGATTGTTTG AAGAACTCCC AAAGTGAATT TGAAAAAGAA 
AATCAGAAGG GAAAAGCTGT TATATTAGAT TTGGAGAAAA CTTGCAAAGA ATTAAAGCAT 
CAGCTTCAAG TACAGACAGA AAGCCTGCAT AAGGAGCAGA ATGAAATGAA AAAGTCACTT 
GAAAAAGAAA AGGAGACTTC TCATCAGTTG AAGTTGGAGC TCAGTTCCAT GCAGGGACAA 
GTCATACAGG CCCAGGACAG CTTAAAACAA AAGGAAAAAG AAGAACAACA GCTTCAGAGT 
AACATAAATG AGCTAAAGCA ATTGACTGAA CAGAAGAAAA AGCAAATTGA AACACTCCAA 
GGAGAAGTTA AAATTGCTGT TTCACAGAAG ACAGAACTTG AGAATAAACT ACAGCAGCAG 
TCAATGCAAG CAGCACAGGA ACTTGCAGCA GAGAAACAAA AAATATCAGT GTTACAAAAC 
ACCTATGAAA AAAGTCAGGA AAATCTAAAA CAGCTTCAGT CGGATTTCTA TGGGAAGGAA 
TCTGAACTAC TTGCCACAAG GCAGGATCTT AAGTCTATAG AAGAGAAGCT TTCTCTAGCA 
CAGGAGGATT TGATATCAAA CAGAAATCAA ATTGGAAACC AAAATAAATT GATTCAAGAA 
CTGAAGACCA CCAAGACTAC TTTGGAGCAA GATTTAGCAA AGAAAGAACA GCAATTGAAG 
GAACAGAATA AAGCACTACA AGATATGCAA AAAGAAAAGT CACTAAAAGA AAAAGAACTA 
GTAAATGAAA AATCTAAATT GGCAGAGACA GAGGAAATTA AGTGTAGACA AGAAAAAGAA 
ATTGCTAAAT TGAGTGAAGA ACTCAAGTCT CACAAGCAGG AAAGCATAAA GGAAATAACA 
AATCTCAAGG ATGCCAAACA GCTTTTGATT CAGCAGAAAT TAGAGCTTCA AGGGAAAGTA 
GATTCCCTGA AGGCAACCCT TGAACAGGAG AAGAAAACCC AGCAAATGCT AAAAGAGCAG 
ATGAAAAAGG AAGAAGATGA GCTGAAGAAA GAGTTTATGG AAAAGGAAGC TAAACTGCAT 
TCCGAAATAA AAGAAAAAGA AGTAGGAATG AAGAAGCATG AAGAAAATGG GGCTAAACTT 
ACCATGCAGA TCACAGCATT AAATGAAAAC TTGGGCACCA TAAAGAAAGA GTGGCAGTCC 
AGTCAACGGA GAGTCAGTGA ACTCGAGAAA CAGACGGACG ACTTACGGGG TGAAATTGCA 
GTACTGGAAG CGACAGTTCA GAATAACCAA GATGAAAGGA GAGCACTGCT GGAAAGGTGT 
CTTAAAGGAG AAGGTGAAAT AGAAAAGCTT CAAACCAAAG TACTAGAATT GCAAAGAAAG 
CTGGATAATA CAACTGCAGC AGTGCAGGAG CTAGGCAGAG AGAATCAGTC CCTTCAAATC 
AAACATACAC AGGCGTTGAC TAGAAAGTGG GCTGAAGACA GTGAAGTACA AAACTGTATG 
GCCTGTGGGA AAGGCTTTTC CGTAACAGTG AGACGGCATC ACTGCAGACA ATGTGGAAAT 
ATCTTCTGTG CTGAGTGTTC AGCCAAAAAT GCCTTAACTC CTTCTTCCAA GAAGCCTGTT 
CGTGTCTGTG ATGGATGTTT CAATGACTTG CAAGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005198275.1
CDS	260..4618
Translation	MLRRILQRTPGRVGSQGSDLDSSAAPINTVDVNNESSSEGFICPLCMKRCITAIDLSEHYQKVHAENETRGQELLNDSSVTGFICPQCMKSLGSADELFKHYEAVHDAGNDSSHGGEALALKRDDITLLRQEVQDLQASLKEEKWYSEELKKELEKFQGLQQQESKPDGLVADSSAELQSLEQQLEEAQTENFNIKQMKDLFEQKAAQLATEIADIKSKYDEERSLREAAEQQVTHLTEELNKEAAVIQDLKTELLQRPGIEDVAVLKKELVQVQTLMDNMTLERERESEKLKDECKKLQTEYTNSEAVISQLRSELAKGPQEVAVYVQELQKLKSSVNELTQKNQNLTEKLQKKELDYTQLEEKHNEECMSKKSIQASLHQKDLDCQQLQSRLSASETSLQRIQAELGEKGEATQKLKEELSEVETKYQHLKAEFKQLQQQREEKDQHGLQLQSEINQLHSKLLETERQLGEAHGRLKEQRQLSSEKLMDKEQQVADLQLKLSRLEEELKEKVTNSTELQHQLDKTKQQHQEQQALQQSTTAKLREAQNDLEQVLRQIGDKDQKIQNLEALLQKSKENISLLEKEREDLYAKIQAGEGETAVLNQLQEKNHTLQEQVTQLTEKLKNQSESHKQAQENLHDQVQEQKAHLRAAQDRVLSLESSINELNSQLNESKEKVSQLDIQVKAKTELLLSAEAAKTAQRADLQNHLDTAQNALQDKQQELNKITTQLDQVTTKLQDKQEHCSQLESHLKEYKEKHLSLEQKTEELEGQIKKLEADMLEVKASKEQALQGLQQQRQLNTDLELRATELSKQLEMEKETVSNTKLDLQKKSEALENTKQMLTKQEEEKTMLKQEIENLSQDAKMQHKELNDRIQTAVTELQKVKVEKDSLVAELSAAKEKLSKVSDCLKNSQSEFEKENQKGKAVILDLEKTCKELKHQLQVQTESLHKEQNEMKKSLEKEKETSHQLKLELSSMQGQVIQAQDSLKQKEKEEQQLQSNINELKQLTEQKKKQIETLQGEVKIAVSQKTELENKLQQQSMQAAQELAAEKQKISVLQNTYEKSQENLKQLQSDFYGKESELLATRQDLKSIEEKLSLAQEDLISNRNQIGNQNKLIQELKTTKTTLEQDLAKKEQQLKEQNKALQDMQKEKSLKEKELVNEKSKLAETEEIKCRQEKEIAKLSEELKSHKQESIKEITNLKDAKQLLIQQKLELQGKVDSLKATLEQEKKTQQMLKEQMKKEEDELKKEFMEKEAKLHSEIKEKEVGMKKHEENGAKLTMQITALNENLGTIKKEWQSSQRRVSELEKQTDDLRGEIAVLEATVQNNQDERRALLERCLKGEGEIEKLQTKVLELQRKLDNTTAAVQELGRENQSLQIKHTQALTRKWAEDSEVQNCMACGKGFSVTVRRHHCRQCGNIFCAECSAKNALTPSSKKPVRVCDGCFNDLQG

Target ORF information:

RefSeq Version	XM_005198218.3
Organism	Bos taurus(cattle)
Definition	Bos taurus early endosome antigen 1 (EEA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005198218.3

ORF Insert Sequence:

ATGTTAAGGA GGATTCTACA GAGGACTCCT GGGAGAGTTG GCTCTCAAGG TTCAGATCTA 
GATTCATCAG CAGCTCCTAT AAACACAGTG GATGTCAATA ATGAAAGCTC TTCAGAGGGT 
TTCATCTGTC CGTTATGTAT GAAAAGATGT ATAACTGCTA TTGATTTGTC GGAGCATTAC 
CAGAAAGTGC ACGCTGAAAA TGAGACAAGA GGACAGGAAC TTCTTAATGA CTCCAGTGTT 
ACTGGTTTCA TATGTCCACA GTGTATGAAA TCTCTTGGAT CTGCTGATGA ACTTTTCAAA 
CACTATGAGG CAGTCCATGA TGCTGGTAAT GACTCAAGTC ACGGAGGAGA AGCTTTGGCT 
CTGAAGCGAG ATGATATAAC ACTACTCAGA CAAGAGGTCC AAGACCTACA GGCTTCACTT 
AAGGAAGAAA AATGGTACTC AGAAGAATTA AAGAAAGAAT TAGAAAAATT TCAAGGGCTG 
CAGCAACAAG AGTCCAAACC TGATGGATTA GTGGCTGATT CATCAGCAGA ACTACAGTCT 
TTGGAACAAC AATTAGAAGA AGCCCAAACA GAAAATTTTA ATATTAAACA AATGAAAGAT 
TTGTTTGAAC AGAAAGCAGC CCAACTTGCT ACTGAAATTG CAGATATAAA GTCAAAATAT 
GATGAAGAAA GGAGTCTTAG AGAAGCTGCT GAACAGCAAG TGACACATCT AACAGAAGAA 
TTAAACAAAG AAGCAGCTGT GATTCAAGAT CTAAAGACTG AACTGCTTCA GAGACCTGGA 
ATAGAAGATG TTGCTGTATT AAAGAAAGAA CTGGTCCAAG TTCAAACACT AATGGATAAC 
ATGACCTTGG AACGTGAGAG AGAATCTGAA AAACTCAAAG ATGAGTGCAA AAAGTTACAA 
ACAGAATATA CTAACTCAGA GGCCGTAATA AGCCAGCTGA GGAGTGAACT TGCCAAAGGC 
CCGCAGGAAG TTGCTGTGTA TGTGCAGGAA CTACAAAAAC TTAAAAGTTC AGTTAATGAA 
TTAACACAAA AAAATCAGAA CTTGACTGAA AAGTTACAGA AGAAAGAACT GGACTATACT 
CAGTTAGAAG AGAAACATAA TGAAGAATGT ATGAGTAAAA AGAGTATACA AGCAAGCCTT 
CATCAAAAAG ACCTGGATTG TCAACAGCTT CAGTCAAGGT TATCTGCATC TGAAACCTCA 
CTGCAGAGAA TACAGGCAGA ACTAGGTGAA AAGGGAGAAG CTACTCAGAA GCTCAAAGAA 
GAATTATCAG AAGTGGAGAC CAAGTACCAG CATCTTAAGG CAGAGTTTAA ACAGCTACAA 
CAACAGAGAG AAGAAAAGGA TCAGCATGGG TTACAACTGC AAAGTGAAAT CAATCAATTA 
CATAGCAAAC TTCTGGAGAC AGAGCGCCAA CTAGGAGAAG CTCATGGAAG GCTGAAGGAA 
CAGAGACAGC TTTCAAGTGA AAAGTTGATG GATAAAGAAC AACAAGTGGC TGATCTACAA 
CTCAAACTTT CTCGTTTAGA GGAAGAGTTG AAGGAAAAAG TAACCAATTC CACAGAATTG 
CAGCATCAGT TAGATAAAAC AAAGCAACAG CATCAAGAAC AACAAGCTCT TCAACAAAGC 
ACTACAGCAA AACTTCGAGA AGCTCAGAAT GATTTGGAAC AAGTACTACG TCAAATTGGT 
GATAAGGACC AAAAGATCCA GAACCTTGAA GCCTTATTAC AGAAGAGCAA AGAAAATATT 
TCTTTACTAG AAAAGGAGAG AGAAGATCTT TATGCAAAAA TTCAGGCTGG TGAGGGAGAG 
ACTGCTGTTC TTAACCAATT ACAAGAAAAA AACCATACAT TACAGGAACA AGTAACTCAG 
CTAACAGAGA AGCTGAAGAA TCAGTCAGAA AGCCATAAAC AAGCCCAGGA GAATTTGCAT 
GACCAGGTGC AGGAGCAGAA GGCACATCTT AGAGCTGCAC AAGACCGTGT CCTTTCCCTA 
GAATCCAGCA TCAATGAATT AAATAGTCAG TTAAATGAAA GCAAGGAGAA GGTTTCCCAG 
CTTGATATAC AGGTTAAAGC CAAAACTGAA TTATTGCTAT CAGCAGAAGC AGCAAAAACT 
GCTCAGAGAG CAGATCTTCA GAATCATCTG GACACAGCCC AAAATGCATT ACAAGATAAA 
CAGCAGGAGT TAAATAAGAT CACTACTCAG TTGGATCAGG TCACTACAAA GTTGCAGGAC 
AAGCAAGAAC ATTGCAGTCA GCTGGAAAGT CATCTTAAAG AATACAAAGA GAAACATCTG 
TCTTTAGAAC AGAAAACTGA AGAATTAGAA GGTCAAATCA AGAAACTAGA AGCTGATATG 
CTTGAAGTTA AAGCAAGCAA GGAGCAAGCT TTGCAGGGTC TACAACAACA GAGACAGCTG 
AACACAGATT TAGAGCTTCG AGCCACAGAA TTGAGTAAAC AACTTGAAAT GGAGAAAGAA 
ACAGTATCTA ATACAAAGTT GGATCTACAG AAGAAATCTG AAGCACTTGA AAATACTAAG 
CAAATGCTTA CCAAGCAAGA AGAAGAAAAA ACAATGCTTA AACAAGAAAT TGAAAATTTA 
AGTCAAGATG CAAAGATGCA GCACAAGGAA CTGAATGATA GGATTCAAAC AGCAGTAACA 
GAACTACAGA AAGTGAAGGT GGAGAAAGAC TCTCTGGTAG CAGAGCTTTC TGCAGCAAAA 
GAGAAACTGT CAAAAGTTTC TGATTGTTTG AAGAACTCCC AAAGTGAATT TGAAAAAGAA 
AATCAGAAGG GAAAAGCTGT TATATTAGAT TTGGAGAAAA CTTGCAAAGA ATTAAAGCAT 
CAGCTTCAAG TACAGACAGA AAGCCTGCAT AAGGAGCAGA ATGAAATGAA AAAGTCACTT 
GAAAAAGAAA AGGAGACTTC TCATCAGTTG AAGTTGGAGC TCAGTTCCAT GCAGGGACAA 
GTCATACAGG CCCAGGACAG CTTAAAACAA AAGGAAAAAG AAGAACAACA GCTTCAGAGT 
AACATAAATG AGCTAAAGCA ATTGACTGAA CAGAAGAAAA AGCAAATTGA AACACTCCAA 
GGAGAAGTTA AAATTGCTGT TTCACAGAAG ACAGAACTTG AGAATAAACT ACAGCAGCAG 
TCAATGCAAG CAGCACAGGA ACTTGCAGCA GAGAAACAAA AAATATCAGT GTTACAAAAC 
ACCTATGAAA AAAGTCAGGA AAATCTAAAA CAGCTTCAGT CGGATTTCTA TGGGAAGGAA 
TCTGAACTAC TTGCCACAAG GCAGGATCTT AAGTCTATAG AAGAGAAGCT TTCTCTAGCA 
CAGGAGGATT TGATATCAAA CAGAAATCAA ATTGGAAACC AAAATAAATT GATTCAAGAA 
CTGAAGACCA CCAAGACTAC TTTGGAGCAA GATTTAGCAA AGAAAGAACA GCAATTGAAG 
GAACAGAATA AAGCACTACA AGATATGCAA AAAGAAAAGT CACTAAAAGA AAAAGAACTA 
GTAAATGAAA AATCTAAATT GGCAGAGACA GAGGAAATTA AGTGTAGACA AGAAAAAGAA 
ATTGCTAAAT TGAGTGAAGA ACTCAAGTCT CACAAGCAGG AAAGCATAAA GGAAATAACA 
AATCTCAAGG ATGCCAAACA GCTTTTGATT CAGCAGAAAT TAGAGCTTCA AGGGAAAGTA 
GATTCCCTGA AGGCAACCCT TGAACAGGAG AAGAAAACCC AGCAAATGCT AAAAGAGCAG 
ATGAAAAAGG AAGAAGATGA GCTGAAGAAA GAGTTTATGG AAAAGGAAGC TAAACTGCAT 
TCCGAAATAA AAGAAAAAGA AGTAGGAATG AAGAAGCATG AAGAAAATGG GGCTAAACTT 
ACCATGCAGA TCACAGCATT AAATGAAAAC TTGGGCACCA TAAAGAAAGA GTGGCAGTCC 
AGTCAACGGA GAGTCAGTGA ACTCGAGAAA CAGACGGACG ACTTACGGGG TGAAATTGCA 
GTACTGGAAG CGACAGTTCA GAATAACCAA GATGAAAGGA GAGCACTGCT GGAAAGGTGT 
CTTAAAGGAG AAGGTGAAAT AGAAAAGCTT CAAACCAAAG TACTAGAATT GCAAAGAAAG 
CTGGATAATA CAACTGCAGC AGTGCAGGAG CTAGGCAGAG AGAATCAGTC CCTTCAAATC 
AAACATACAC AGGCGTTGAC TAGAAAGTGG GCTGAAGACA GTGAAGTACA AAACTGTATG 
GCCTGTGGGA AAGGCTTTTC CGTAACAGTG AGACGGCATC ACTGCAGACA ATGTGGAAAT 
ATCTTCTGTG CTGAGTGTTC AGCCAAAAAT GCCTTAACTC CTTCTTCCAA GAAGCCTGTT 
CGTGTCTGTG ATGGATGTTT CAATGACTTG CAAGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EEA1 ( XM_005206125.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005206182.1 Bos taurus early endosome antigen 1 (EEA1), transcript variant X1, mRNA.

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CloneID	OBa04184
Clone ID Related Accession (Same CDS sequence)	XM_005198218.3 , XM_005206125.3 , XM_005206125.4
Accession Version	XM_005206125.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	early endosome antigen 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103917.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005206125.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTAAGGA GGATTCTACA GAGGACTCCT GGGAGAGTTG GCTCTCAAGG TTCAGATCTA 
GATTCATCAG CAGCTCCTAT AAACACAGTG GATGTCAATA ATGAAAGCTC TTCAGAGGGT 
TTCATCTGTC CGTTATGTAT GAAAAGATGT ATAACTGCTA TTGATTTGTC GGAGCATTAC 
CAGAAAGTGC ACGCTGAAAA TGAGACAAGA GGACAGGAAC TTCTTAATGA CTCCAGTGTT 
ACTGGTTTCA TATGTCCACA GTGTATGAAA TCTCTTGGAT CTGCTGATGA ACTTTTCAAA 
CACTATGAGG CAGTCCATGA TGCTGGTAAT GACTCAAGTC ACGGAGGAGA AGCTTTGGCT 
CTGAAGCGAG ATGATATAAC ACTACTCAGA CAAGAGGTCC AAGACCTACA GGCTTCACTT 
AAGGAAGAAA AATGGTACTC AGAAGAATTA AAGAAAGAAT TAGAAAAATT TCAAGGGCTG 
CAGCAACAAG AGTCCAAACC TGATGGATTA GTGGCTGATT CATCAGCAGA ACTACAGTCT 
TTGGAACAAC AATTAGAAGA AGCCCAAACA GAAAATTTTA ATATTAAACA AATGAAAGAT 
TTGTTTGAAC AGAAAGCAGC CCAACTTGCT ACTGAAATTG CAGATATAAA GTCAAAATAT 
GATGAAGAAA GGAGTCTTAG AGAAGCTGCT GAACAGCAAG TGACACATCT AACAGAAGAA 
TTAAACAAAG AAGCAGCTGT GATTCAAGAT CTAAAGACTG AACTGCTTCA GAGACCTGGA 
ATAGAAGATG TTGCTGTATT AAAGAAAGAA CTGGTCCAAG TTCAAACACT AATGGATAAC 
ATGACCTTGG AACGTGAGAG AGAATCTGAA AAACTCAAAG ATGAGTGCAA AAAGTTACAA 
ACAGAATATA CTAACTCAGA GGCCGTAATA AGCCAGCTGA GGAGTGAACT TGCCAAAGGC 
CCGCAGGAAG TTGCTGTGTA TGTGCAGGAA CTACAAAAAC TTAAAAGTTC AGTTAATGAA 
TTAACACAAA AAAATCAGAA CTTGACTGAA AAGTTACAGA AGAAAGAACT GGACTATACT 
CAGTTAGAAG AGAAACATAA TGAAGAATGT ATGAGTAAAA AGAGTATACA AGCAAGCCTT 
CATCAAAAAG ACCTGGATTG TCAACAGCTT CAGTCAAGGT TATCTGCATC TGAAACCTCA 
CTGCAGAGAA TACAGGCAGA ACTAGGTGAA AAGGGAGAAG CTACTCAGAA GCTCAAAGAA 
GAATTATCAG AAGTGGAGAC CAAGTACCAG CATCTTAAGG CAGAGTTTAA ACAGCTACAA 
CAACAGAGAG AAGAAAAGGA TCAGCATGGG TTACAACTGC AAAGTGAAAT CAATCAATTA 
CATAGCAAAC TTCTGGAGAC AGAGCGCCAA CTAGGAGAAG CTCATGGAAG GCTGAAGGAA 
CAGAGACAGC TTTCAAGTGA AAAGTTGATG GATAAAGAAC AACAAGTGGC TGATCTACAA 
CTCAAACTTT CTCGTTTAGA GGAAGAGTTG AAGGAAAAAG TAACCAATTC CACAGAATTG 
CAGCATCAGT TAGATAAAAC AAAGCAACAG CATCAAGAAC AACAAGCTCT TCAACAAAGC 
ACTACAGCAA AACTTCGAGA AGCTCAGAAT GATTTGGAAC AAGTACTACG TCAAATTGGT 
GATAAGGACC AAAAGATCCA GAACCTTGAA GCCTTATTAC AGAAGAGCAA AGAAAATATT 
TCTTTACTAG AAAAGGAGAG AGAAGATCTT TATGCAAAAA TTCAGGCTGG TGAGGGAGAG 
ACTGCTGTTC TTAACCAATT ACAAGAAAAA AACCATACAT TACAGGAACA AGTAACTCAG 
CTAACAGAGA AGCTGAAGAA TCAGTCAGAA AGCCATAAAC AAGCCCAGGA GAATTTGCAT 
GACCAGGTGC AGGAGCAGAA GGCACATCTT AGAGCTGCAC AAGACCGTGT CCTTTCCCTA 
GAATCCAGCA TCAATGAATT AAATAGTCAG TTAAATGAAA GCAAGGAGAA GGTTTCCCAG 
CTTGATATAC AGGTTAAAGC CAAAACTGAA TTATTGCTAT CAGCAGAAGC AGCAAAAACT 
GCTCAGAGAG CAGATCTTCA GAATCATCTG GACACAGCCC AAAATGCATT ACAAGATAAA 
CAGCAGGAGT TAAATAAGAT CACTACTCAG TTGGATCAGG TCACTACAAA GTTGCAGGAC 
AAGCAAGAAC ATTGCAGTCA GCTGGAAAGT CATCTTAAAG AATACAAAGA GAAACATCTG 
TCTTTAGAAC AGAAAACTGA AGAATTAGAA GGTCAAATCA AGAAACTAGA AGCTGATATG 
CTTGAAGTTA AAGCAAGCAA GGAGCAAGCT TTGCAGGGTC TACAACAACA GAGACAGCTG 
AACACAGATT TAGAGCTTCG AGCCACAGAA TTGAGTAAAC AACTTGAAAT GGAGAAAGAA 
ACAGTATCTA ATACAAAGTT GGATCTACAG AAGAAATCTG AAGCACTTGA AAATACTAAG 
CAAATGCTTA CCAAGCAAGA AGAAGAAAAA ACAATGCTTA AACAAGAAAT TGAAAATTTA 
AGTCAAGATG CAAAGATGCA GCACAAGGAA CTGAATGATA GGATTCAAAC AGCAGTAACA 
GAACTACAGA AAGTGAAGGT GGAGAAAGAC TCTCTGGTAG CAGAGCTTTC TGCAGCAAAA 
GAGAAACTGT CAAAAGTTTC TGATTGTTTG AAGAACTCCC AAAGTGAATT TGAAAAAGAA 
AATCAGAAGG GAAAAGCTGT TATATTAGAT TTGGAGAAAA CTTGCAAAGA ATTAAAGCAT 
CAGCTTCAAG TACAGACAGA AAGCCTGCAT AAGGAGCAGA ATGAAATGAA AAAGTCACTT 
GAAAAAGAAA AGGAGACTTC TCATCAGTTG AAGTTGGAGC TCAGTTCCAT GCAGGGACAA 
GTCATACAGG CCCAGGACAG CTTAAAACAA AAGGAAAAAG AAGAACAACA GCTTCAGAGT 
AACATAAATG AGCTAAAGCA ATTGACTGAA CAGAAGAAAA AGCAAATTGA AACACTCCAA 
GGAGAAGTTA AAATTGCTGT TTCACAGAAG ACAGAACTTG AGAATAAACT ACAGCAGCAG 
TCAATGCAAG CAGCACAGGA ACTTGCAGCA GAGAAACAAA AAATATCAGT GTTACAAAAC 
ACCTATGAAA AAAGTCAGGA AAATCTAAAA CAGCTTCAGT CGGATTTCTA TGGGAAGGAA 
TCTGAACTAC TTGCCACAAG GCAGGATCTT AAGTCTATAG AAGAGAAGCT TTCTCTAGCA 
CAGGAGGATT TGATATCAAA CAGAAATCAA ATTGGAAACC AAAATAAATT GATTCAAGAA 
CTGAAGACCA CCAAGACTAC TTTGGAGCAA GATTTAGCAA AGAAAGAACA GCAATTGAAG 
GAACAGAATA AAGCACTACA AGATATGCAA AAAGAAAAGT CACTAAAAGA AAAAGAACTA 
GTAAATGAAA AATCTAAATT GGCAGAGACA GAGGAAATTA AGTGTAGACA AGAAAAAGAA 
ATTGCTAAAT TGAGTGAAGA ACTCAAGTCT CACAAGCAGG AAAGCATAAA GGAAATAACA 
AATCTCAAGG ATGCCAAACA GCTTTTGATT CAGCAGAAAT TAGAGCTTCA AGGGAAAGTA 
GATTCCCTGA AGGCAACCCT TGAACAGGAG AAGAAAACCC AGCAAATGCT AAAAGAGCAG 
ATGAAAAAGG AAGAAGATGA GCTGAAGAAA GAGTTTATGG AAAAGGAAGC TAAACTGCAT 
TCCGAAATAA AAGAAAAAGA AGTAGGAATG AAGAAGCATG AAGAAAATGG GGCTAAACTT 
ACCATGCAGA TCACAGCATT AAATGAAAAC TTGGGCACCA TAAAGAAAGA GTGGCAGTCC 
AGTCAACGGA GAGTCAGTGA ACTCGAGAAA CAGACGGACG ACTTACGGGG TGAAATTGCA 
GTACTGGAAG CGACAGTTCA GAATAACCAA GATGAAAGGA GAGCACTGCT GGAAAGGTGT 
CTTAAAGGAG AAGGTGAAAT AGAAAAGCTT CAAACCAAAG TACTAGAATT GCAAAGAAAG 
CTGGATAATA CAACTGCAGC AGTGCAGGAG CTAGGCAGAG AGAATCAGTC CCTTCAAATC 
AAACATACAC AGGCGTTGAC TAGAAAGTGG GCTGAAGACA GTGAAGTACA AAACTGTATG 
GCCTGTGGGA AAGGCTTTTC CGTAACAGTG AGACGGCATC ACTGCAGACA ATGTGGAAAT 
ATCTTCTGTG CTGAGTGTTC AGCCAAAAAT GCCTTAACTC CTTCTTCCAA GAAGCCTGTT 
CGTGTCTGTG ATGGATGTTT CAATGACTTG CAAGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005206182.1
CDS	260..4618
Translation	MLRRILQRTPGRVGSQGSDLDSSAAPINTVDVNNESSSEGFICPLCMKRCITAIDLSEHYQKVHAENETRGQELLNDSSVTGFICPQCMKSLGSADELFKHYEAVHDAGNDSSHGGEALALKRDDITLLRQEVQDLQASLKEEKWYSEELKKELEKFQGLQQQESKPDGLVADSSAELQSLEQQLEEAQTENFNIKQMKDLFEQKAAQLATEIADIKSKYDEERSLREAAEQQVTHLTEELNKEAAVIQDLKTELLQRPGIEDVAVLKKELVQVQTLMDNMTLERERESEKLKDECKKLQTEYTNSEAVISQLRSELAKGPQEVAVYVQELQKLKSSVNELTQKNQNLTEKLQKKELDYTQLEEKHNEECMSKKSIQASLHQKDLDCQQLQSRLSASETSLQRIQAELGEKGEATQKLKEELSEVETKYQHLKAEFKQLQQQREEKDQHGLQLQSEINQLHSKLLETERQLGEAHGRLKEQRQLSSEKLMDKEQQVADLQLKLSRLEEELKEKVTNSTELQHQLDKTKQQHQEQQALQQSTTAKLREAQNDLEQVLRQIGDKDQKIQNLEALLQKSKENISLLEKEREDLYAKIQAGEGETAVLNQLQEKNHTLQEQVTQLTEKLKNQSESHKQAQENLHDQVQEQKAHLRAAQDRVLSLESSINELNSQLNESKEKVSQLDIQVKAKTELLLSAEAAKTAQRADLQNHLDTAQNALQDKQQELNKITTQLDQVTTKLQDKQEHCSQLESHLKEYKEKHLSLEQKTEELEGQIKKLEADMLEVKASKEQALQGLQQQRQLNTDLELRATELSKQLEMEKETVSNTKLDLQKKSEALENTKQMLTKQEEEKTMLKQEIENLSQDAKMQHKELNDRIQTAVTELQKVKVEKDSLVAELSAAKEKLSKVSDCLKNSQSEFEKENQKGKAVILDLEKTCKELKHQLQVQTESLHKEQNEMKKSLEKEKETSHQLKLELSSMQGQVIQAQDSLKQKEKEEQQLQSNINELKQLTEQKKKQIETLQGEVKIAVSQKTELENKLQQQSMQAAQELAAEKQKISVLQNTYEKSQENLKQLQSDFYGKESELLATRQDLKSIEEKLSLAQEDLISNRNQIGNQNKLIQELKTTKTTLEQDLAKKEQQLKEQNKALQDMQKEKSLKEKELVNEKSKLAETEEIKCRQEKEIAKLSEELKSHKQESIKEITNLKDAKQLLIQQKLELQGKVDSLKATLEQEKKTQQMLKEQMKKEEDELKKEFMEKEAKLHSEIKEKEVGMKKHEENGAKLTMQITALNENLGTIKKEWQSSQRRVSELEKQTDDLRGEIAVLEATVQNNQDERRALLERCLKGEGEIEKLQTKVLELQRKLDNTTAAVQELGRENQSLQIKHTQALTRKWAEDSEVQNCMACGKGFSVTVRRHHCRQCGNIFCAECSAKNALTPSSKKPVRVCDGCFNDLQG

Target ORF information:

RefSeq Version	XM_005206125.3
Organism	Bos taurus(cattle)
Definition	Bos taurus early endosome antigen 1 (EEA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005206125.3

ORF Insert Sequence:

ATGTTAAGGA GGATTCTACA GAGGACTCCT GGGAGAGTTG GCTCTCAAGG TTCAGATCTA 
GATTCATCAG CAGCTCCTAT AAACACAGTG GATGTCAATA ATGAAAGCTC TTCAGAGGGT 
TTCATCTGTC CGTTATGTAT GAAAAGATGT ATAACTGCTA TTGATTTGTC GGAGCATTAC 
CAGAAAGTGC ACGCTGAAAA TGAGACAAGA GGACAGGAAC TTCTTAATGA CTCCAGTGTT 
ACTGGTTTCA TATGTCCACA GTGTATGAAA TCTCTTGGAT CTGCTGATGA ACTTTTCAAA 
CACTATGAGG CAGTCCATGA TGCTGGTAAT GACTCAAGTC ACGGAGGAGA AGCTTTGGCT 
CTGAAGCGAG ATGATATAAC ACTACTCAGA CAAGAGGTCC AAGACCTACA GGCTTCACTT 
AAGGAAGAAA AATGGTACTC AGAAGAATTA AAGAAAGAAT TAGAAAAATT TCAAGGGCTG 
CAGCAACAAG AGTCCAAACC TGATGGATTA GTGGCTGATT CATCAGCAGA ACTACAGTCT 
TTGGAACAAC AATTAGAAGA AGCCCAAACA GAAAATTTTA ATATTAAACA AATGAAAGAT 
TTGTTTGAAC AGAAAGCAGC CCAACTTGCT ACTGAAATTG CAGATATAAA GTCAAAATAT 
GATGAAGAAA GGAGTCTTAG AGAAGCTGCT GAACAGCAAG TGACACATCT AACAGAAGAA 
TTAAACAAAG AAGCAGCTGT GATTCAAGAT CTAAAGACTG AACTGCTTCA GAGACCTGGA 
ATAGAAGATG TTGCTGTATT AAAGAAAGAA CTGGTCCAAG TTCAAACACT AATGGATAAC 
ATGACCTTGG AACGTGAGAG AGAATCTGAA AAACTCAAAG ATGAGTGCAA AAAGTTACAA 
ACAGAATATA CTAACTCAGA GGCCGTAATA AGCCAGCTGA GGAGTGAACT TGCCAAAGGC 
CCGCAGGAAG TTGCTGTGTA TGTGCAGGAA CTACAAAAAC TTAAAAGTTC AGTTAATGAA 
TTAACACAAA AAAATCAGAA CTTGACTGAA AAGTTACAGA AGAAAGAACT GGACTATACT 
CAGTTAGAAG AGAAACATAA TGAAGAATGT ATGAGTAAAA AGAGTATACA AGCAAGCCTT 
CATCAAAAAG ACCTGGATTG TCAACAGCTT CAGTCAAGGT TATCTGCATC TGAAACCTCA 
CTGCAGAGAA TACAGGCAGA ACTAGGTGAA AAGGGAGAAG CTACTCAGAA GCTCAAAGAA 
GAATTATCAG AAGTGGAGAC CAAGTACCAG CATCTTAAGG CAGAGTTTAA ACAGCTACAA 
CAACAGAGAG AAGAAAAGGA TCAGCATGGG TTACAACTGC AAAGTGAAAT CAATCAATTA 
CATAGCAAAC TTCTGGAGAC AGAGCGCCAA CTAGGAGAAG CTCATGGAAG GCTGAAGGAA 
CAGAGACAGC TTTCAAGTGA AAAGTTGATG GATAAAGAAC AACAAGTGGC TGATCTACAA 
CTCAAACTTT CTCGTTTAGA GGAAGAGTTG AAGGAAAAAG TAACCAATTC CACAGAATTG 
CAGCATCAGT TAGATAAAAC AAAGCAACAG CATCAAGAAC AACAAGCTCT TCAACAAAGC 
ACTACAGCAA AACTTCGAGA AGCTCAGAAT GATTTGGAAC AAGTACTACG TCAAATTGGT 
GATAAGGACC AAAAGATCCA GAACCTTGAA GCCTTATTAC AGAAGAGCAA AGAAAATATT 
TCTTTACTAG AAAAGGAGAG AGAAGATCTT TATGCAAAAA TTCAGGCTGG TGAGGGAGAG 
ACTGCTGTTC TTAACCAATT ACAAGAAAAA AACCATACAT TACAGGAACA AGTAACTCAG 
CTAACAGAGA AGCTGAAGAA TCAGTCAGAA AGCCATAAAC AAGCCCAGGA GAATTTGCAT 
GACCAGGTGC AGGAGCAGAA GGCACATCTT AGAGCTGCAC AAGACCGTGT CCTTTCCCTA 
GAATCCAGCA TCAATGAATT AAATAGTCAG TTAAATGAAA GCAAGGAGAA GGTTTCCCAG 
CTTGATATAC AGGTTAAAGC CAAAACTGAA TTATTGCTAT CAGCAGAAGC AGCAAAAACT 
GCTCAGAGAG CAGATCTTCA GAATCATCTG GACACAGCCC AAAATGCATT ACAAGATAAA 
CAGCAGGAGT TAAATAAGAT CACTACTCAG TTGGATCAGG TCACTACAAA GTTGCAGGAC 
AAGCAAGAAC ATTGCAGTCA GCTGGAAAGT CATCTTAAAG AATACAAAGA GAAACATCTG 
TCTTTAGAAC AGAAAACTGA AGAATTAGAA GGTCAAATCA AGAAACTAGA AGCTGATATG 
CTTGAAGTTA AAGCAAGCAA GGAGCAAGCT TTGCAGGGTC TACAACAACA GAGACAGCTG 
AACACAGATT TAGAGCTTCG AGCCACAGAA TTGAGTAAAC AACTTGAAAT GGAGAAAGAA 
ACAGTATCTA ATACAAAGTT GGATCTACAG AAGAAATCTG AAGCACTTGA AAATACTAAG 
CAAATGCTTA CCAAGCAAGA AGAAGAAAAA ACAATGCTTA AACAAGAAAT TGAAAATTTA 
AGTCAAGATG CAAAGATGCA GCACAAGGAA CTGAATGATA GGATTCAAAC AGCAGTAACA 
GAACTACAGA AAGTGAAGGT GGAGAAAGAC TCTCTGGTAG CAGAGCTTTC TGCAGCAAAA 
GAGAAACTGT CAAAAGTTTC TGATTGTTTG AAGAACTCCC AAAGTGAATT TGAAAAAGAA 
AATCAGAAGG GAAAAGCTGT TATATTAGAT TTGGAGAAAA CTTGCAAAGA ATTAAAGCAT 
CAGCTTCAAG TACAGACAGA AAGCCTGCAT AAGGAGCAGA ATGAAATGAA AAAGTCACTT 
GAAAAAGAAA AGGAGACTTC TCATCAGTTG AAGTTGGAGC TCAGTTCCAT GCAGGGACAA 
GTCATACAGG CCCAGGACAG CTTAAAACAA AAGGAAAAAG AAGAACAACA GCTTCAGAGT 
AACATAAATG AGCTAAAGCA ATTGACTGAA CAGAAGAAAA AGCAAATTGA AACACTCCAA 
GGAGAAGTTA AAATTGCTGT TTCACAGAAG ACAGAACTTG AGAATAAACT ACAGCAGCAG 
TCAATGCAAG CAGCACAGGA ACTTGCAGCA GAGAAACAAA AAATATCAGT GTTACAAAAC 
ACCTATGAAA AAAGTCAGGA AAATCTAAAA CAGCTTCAGT CGGATTTCTA TGGGAAGGAA 
TCTGAACTAC TTGCCACAAG GCAGGATCTT AAGTCTATAG AAGAGAAGCT TTCTCTAGCA 
CAGGAGGATT TGATATCAAA CAGAAATCAA ATTGGAAACC AAAATAAATT GATTCAAGAA 
CTGAAGACCA CCAAGACTAC TTTGGAGCAA GATTTAGCAA AGAAAGAACA GCAATTGAAG 
GAACAGAATA AAGCACTACA AGATATGCAA AAAGAAAAGT CACTAAAAGA AAAAGAACTA 
GTAAATGAAA AATCTAAATT GGCAGAGACA GAGGAAATTA AGTGTAGACA AGAAAAAGAA 
ATTGCTAAAT TGAGTGAAGA ACTCAAGTCT CACAAGCAGG AAAGCATAAA GGAAATAACA 
AATCTCAAGG ATGCCAAACA GCTTTTGATT CAGCAGAAAT TAGAGCTTCA AGGGAAAGTA 
GATTCCCTGA AGGCAACCCT TGAACAGGAG AAGAAAACCC AGCAAATGCT AAAAGAGCAG 
ATGAAAAAGG AAGAAGATGA GCTGAAGAAA GAGTTTATGG AAAAGGAAGC TAAACTGCAT 
TCCGAAATAA AAGAAAAAGA AGTAGGAATG AAGAAGCATG AAGAAAATGG GGCTAAACTT 
ACCATGCAGA TCACAGCATT AAATGAAAAC TTGGGCACCA TAAAGAAAGA GTGGCAGTCC 
AGTCAACGGA GAGTCAGTGA ACTCGAGAAA CAGACGGACG ACTTACGGGG TGAAATTGCA 
GTACTGGAAG CGACAGTTCA GAATAACCAA GATGAAAGGA GAGCACTGCT GGAAAGGTGT 
CTTAAAGGAG AAGGTGAAAT AGAAAAGCTT CAAACCAAAG TACTAGAATT GCAAAGAAAG 
CTGGATAATA CAACTGCAGC AGTGCAGGAG CTAGGCAGAG AGAATCAGTC CCTTCAAATC 
AAACATACAC AGGCGTTGAC TAGAAAGTGG GCTGAAGACA GTGAAGTACA AAACTGTATG 
GCCTGTGGGA AAGGCTTTTC CGTAACAGTG AGACGGCATC ACTGCAGACA ATGTGGAAAT 
ATCTTCTGTG CTGAGTGTTC AGCCAAAAAT GCCTTAACTC CTTCTTCCAA GAAGCCTGTT 
CGTGTCTGTG ATGGATGTTT CAATGACTTG CAAGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EEA1 ( XM_005206125.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005206182.1 Bos taurus early endosome antigen 1 (EEA1), transcript variant X2, mRNA.

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CloneID	OBa04184
Clone ID Related Accession (Same CDS sequence)	XM_005198218.3 , XM_005206125.3 , XM_005206125.4
Accession Version	XM_005206125.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	early endosome antigen 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005206125.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTAAGGA GGATTCTACA GAGGACTCCT GGGAGAGTTG GCTCTCAAGG TTCAGATCTA 
GATTCATCAG CAGCTCCTAT AAACACAGTG GATGTCAATA ATGAAAGCTC TTCAGAGGGT 
TTCATCTGTC CGTTATGTAT GAAAAGATGT ATAACTGCTA TTGATTTGTC GGAGCATTAC 
CAGAAAGTGC ACGCTGAAAA TGAGACAAGA GGACAGGAAC TTCTTAATGA CTCCAGTGTT 
ACTGGTTTCA TATGTCCACA GTGTATGAAA TCTCTTGGAT CTGCTGATGA ACTTTTCAAA 
CACTATGAGG CAGTCCATGA TGCTGGTAAT GACTCAAGTC ACGGAGGAGA AGCTTTGGCT 
CTGAAGCGAG ATGATATAAC ACTACTCAGA CAAGAGGTCC AAGACCTACA GGCTTCACTT 
AAGGAAGAAA AATGGTACTC AGAAGAATTA AAGAAAGAAT TAGAAAAATT TCAAGGGCTG 
CAGCAACAAG AGTCCAAACC TGATGGATTA GTGGCTGATT CATCAGCAGA ACTACAGTCT 
TTGGAACAAC AATTAGAAGA AGCCCAAACA GAAAATTTTA ATATTAAACA AATGAAAGAT 
TTGTTTGAAC AGAAAGCAGC CCAACTTGCT ACTGAAATTG CAGATATAAA GTCAAAATAT 
GATGAAGAAA GGAGTCTTAG AGAAGCTGCT GAACAGCAAG TGACACATCT AACAGAAGAA 
TTAAACAAAG AAGCAGCTGT GATTCAAGAT CTAAAGACTG AACTGCTTCA GAGACCTGGA 
ATAGAAGATG TTGCTGTATT AAAGAAAGAA CTGGTCCAAG TTCAAACACT AATGGATAAC 
ATGACCTTGG AACGTGAGAG AGAATCTGAA AAACTCAAAG ATGAGTGCAA AAAGTTACAA 
ACAGAATATA CTAACTCAGA GGCCGTAATA AGCCAGCTGA GGAGTGAACT TGCCAAAGGC 
CCGCAGGAAG TTGCTGTGTA TGTGCAGGAA CTACAAAAAC TTAAAAGTTC AGTTAATGAA 
TTAACACAAA AAAATCAGAA CTTGACTGAA AAGTTACAGA AGAAAGAACT GGACTATACT 
CAGTTAGAAG AGAAACATAA TGAAGAATGT ATGAGTAAAA AGAGTATACA AGCAAGCCTT 
CATCAAAAAG ACCTGGATTG TCAACAGCTT CAGTCAAGGT TATCTGCATC TGAAACCTCA 
CTGCAGAGAA TACAGGCAGA ACTAGGTGAA AAGGGAGAAG CTACTCAGAA GCTCAAAGAA 
GAATTATCAG AAGTGGAGAC CAAGTACCAG CATCTTAAGG CAGAGTTTAA ACAGCTACAA 
CAACAGAGAG AAGAAAAGGA TCAGCATGGG TTACAACTGC AAAGTGAAAT CAATCAATTA 
CATAGCAAAC TTCTGGAGAC AGAGCGCCAA CTAGGAGAAG CTCATGGAAG GCTGAAGGAA 
CAGAGACAGC TTTCAAGTGA AAAGTTGATG GATAAAGAAC AACAAGTGGC TGATCTACAA 
CTCAAACTTT CTCGTTTAGA GGAAGAGTTG AAGGAAAAAG TAACCAATTC CACAGAATTG 
CAGCATCAGT TAGATAAAAC AAAGCAACAG CATCAAGAAC AACAAGCTCT TCAACAAAGC 
ACTACAGCAA AACTTCGAGA AGCTCAGAAT GATTTGGAAC AAGTACTACG TCAAATTGGT 
GATAAGGACC AAAAGATCCA GAACCTTGAA GCCTTATTAC AGAAGAGCAA AGAAAATATT 
TCTTTACTAG AAAAGGAGAG AGAAGATCTT TATGCAAAAA TTCAGGCTGG TGAGGGAGAG 
ACTGCTGTTC TTAACCAATT ACAAGAAAAA AACCATACAT TACAGGAACA AGTAACTCAG 
CTAACAGAGA AGCTGAAGAA TCAGTCAGAA AGCCATAAAC AAGCCCAGGA GAATTTGCAT 
GACCAGGTGC AGGAGCAGAA GGCACATCTT AGAGCTGCAC AAGACCGTGT CCTTTCCCTA 
GAATCCAGCA TCAATGAATT AAATAGTCAG TTAAATGAAA GCAAGGAGAA GGTTTCCCAG 
CTTGATATAC AGGTTAAAGC CAAAACTGAA TTATTGCTAT CAGCAGAAGC AGCAAAAACT 
GCTCAGAGAG CAGATCTTCA GAATCATCTG GACACAGCCC AAAATGCATT ACAAGATAAA 
CAGCAGGAGT TAAATAAGAT CACTACTCAG TTGGATCAGG TCACTACAAA GTTGCAGGAC 
AAGCAAGAAC ATTGCAGTCA GCTGGAAAGT CATCTTAAAG AATACAAAGA GAAACATCTG 
TCTTTAGAAC AGAAAACTGA AGAATTAGAA GGTCAAATCA AGAAACTAGA AGCTGATATG 
CTTGAAGTTA AAGCAAGCAA GGAGCAAGCT TTGCAGGGTC TACAACAACA GAGACAGCTG 
AACACAGATT TAGAGCTTCG AGCCACAGAA TTGAGTAAAC AACTTGAAAT GGAGAAAGAA 
ACAGTATCTA ATACAAAGTT GGATCTACAG AAGAAATCTG AAGCACTTGA AAATACTAAG 
CAAATGCTTA CCAAGCAAGA AGAAGAAAAA ACAATGCTTA AACAAGAAAT TGAAAATTTA 
AGTCAAGATG CAAAGATGCA GCACAAGGAA CTGAATGATA GGATTCAAAC AGCAGTAACA 
GAACTACAGA AAGTGAAGGT GGAGAAAGAC TCTCTGGTAG CAGAGCTTTC TGCAGCAAAA 
GAGAAACTGT CAAAAGTTTC TGATTGTTTG AAGAACTCCC AAAGTGAATT TGAAAAAGAA 
AATCAGAAGG GAAAAGCTGT TATATTAGAT TTGGAGAAAA CTTGCAAAGA ATTAAAGCAT 
CAGCTTCAAG TACAGACAGA AAGCCTGCAT AAGGAGCAGA ATGAAATGAA AAAGTCACTT 
GAAAAAGAAA AGGAGACTTC TCATCAGTTG AAGTTGGAGC TCAGTTCCAT GCAGGGACAA 
GTCATACAGG CCCAGGACAG CTTAAAACAA AAGGAAAAAG AAGAACAACA GCTTCAGAGT 
AACATAAATG AGCTAAAGCA ATTGACTGAA CAGAAGAAAA AGCAAATTGA AACACTCCAA 
GGAGAAGTTA AAATTGCTGT TTCACAGAAG ACAGAACTTG AGAATAAACT ACAGCAGCAG 
TCAATGCAAG CAGCACAGGA ACTTGCAGCA GAGAAACAAA AAATATCAGT GTTACAAAAC 
ACCTATGAAA AAAGTCAGGA AAATCTAAAA CAGCTTCAGT CGGATTTCTA TGGGAAGGAA 
TCTGAACTAC TTGCCACAAG GCAGGATCTT AAGTCTATAG AAGAGAAGCT TTCTCTAGCA 
CAGGAGGATT TGATATCAAA CAGAAATCAA ATTGGAAACC AAAATAAATT GATTCAAGAA 
CTGAAGACCA CCAAGACTAC TTTGGAGCAA GATTTAGCAA AGAAAGAACA GCAATTGAAG 
GAACAGAATA AAGCACTACA AGATATGCAA AAAGAAAAGT CACTAAAAGA AAAAGAACTA 
GTAAATGAAA AATCTAAATT GGCAGAGACA GAGGAAATTA AGTGTAGACA AGAAAAAGAA 
ATTGCTAAAT TGAGTGAAGA ACTCAAGTCT CACAAGCAGG AAAGCATAAA GGAAATAACA 
AATCTCAAGG ATGCCAAACA GCTTTTGATT CAGCAGAAAT TAGAGCTTCA AGGGAAAGTA 
GATTCCCTGA AGGCAACCCT TGAACAGGAG AAGAAAACCC AGCAAATGCT AAAAGAGCAG 
ATGAAAAAGG AAGAAGATGA GCTGAAGAAA GAGTTTATGG AAAAGGAAGC TAAACTGCAT 
TCCGAAATAA AAGAAAAAGA AGTAGGAATG AAGAAGCATG AAGAAAATGG GGCTAAACTT 
ACCATGCAGA TCACAGCATT AAATGAAAAC TTGGGCACCA TAAAGAAAGA GTGGCAGTCC 
AGTCAACGGA GAGTCAGTGA ACTCGAGAAA CAGACGGACG ACTTACGGGG TGAAATTGCA 
GTACTGGAAG CGACAGTTCA GAATAACCAA GATGAAAGGA GAGCACTGCT GGAAAGGTGT 
CTTAAAGGAG AAGGTGAAAT AGAAAAGCTT CAAACCAAAG TACTAGAATT GCAAAGAAAG 
CTGGATAATA CAACTGCAGC AGTGCAGGAG CTAGGCAGAG AGAATCAGTC CCTTCAAATC 
AAACATACAC AGGCGTTGAC TAGAAAGTGG GCTGAAGACA GTGAAGTACA AAACTGTATG 
GCCTGTGGGA AAGGCTTTTC CGTAACAGTG AGACGGCATC ACTGCAGACA ATGTGGAAAT 
ATCTTCTGTG CTGAGTGTTC AGCCAAAAAT GCCTTAACTC CTTCTTCCAA GAAGCCTGTT 
CGTGTCTGTG ATGGATGTTT CAATGACTTG CAAGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005206182.1
CDS	275..4633
Translation	MLRRILQRTPGRVGSQGSDLDSSAAPINTVDVNNESSSEGFICPLCMKRCITAIDLSEHYQKVHAENETRGQELLNDSSVTGFICPQCMKSLGSADELFKHYEAVHDAGNDSSHGGEALALKRDDITLLRQEVQDLQASLKEEKWYSEELKKELEKFQGLQQQESKPDGLVADSSAELQSLEQQLEEAQTENFNIKQMKDLFEQKAAQLATEIADIKSKYDEERSLREAAEQQVTHLTEELNKEAAVIQDLKTELLQRPGIEDVAVLKKELVQVQTLMDNMTLERERESEKLKDECKKLQTEYTNSEAVISQLRSELAKGPQEVAVYVQELQKLKSSVNELTQKNQNLTEKLQKKELDYTQLEEKHNEECMSKKSIQASLHQKDLDCQQLQSRLSASETSLQRIQAELGEKGEATQKLKEELSEVETKYQHLKAEFKQLQQQREEKDQHGLQLQSEINQLHSKLLETERQLGEAHGRLKEQRQLSSEKLMDKEQQVADLQLKLSRLEEELKEKVTNSTELQHQLDKTKQQHQEQQALQQSTTAKLREAQNDLEQVLRQIGDKDQKIQNLEALLQKSKENISLLEKEREDLYAKIQAGEGETAVLNQLQEKNHTLQEQVTQLTEKLKNQSESHKQAQENLHDQVQEQKAHLRAAQDRVLSLESSINELNSQLNESKEKVSQLDIQVKAKTELLLSAEAAKTAQRADLQNHLDTAQNALQDKQQELNKITTQLDQVTTKLQDKQEHCSQLESHLKEYKEKHLSLEQKTEELEGQIKKLEADMLEVKASKEQALQGLQQQRQLNTDLELRATELSKQLEMEKETVSNTKLDLQKKSEALENTKQMLTKQEEEKTMLKQEIENLSQDAKMQHKELNDRIQTAVTELQKVKVEKDSLVAELSAAKEKLSKVSDCLKNSQSEFEKENQKGKAVILDLEKTCKELKHQLQVQTESLHKEQNEMKKSLEKEKETSHQLKLELSSMQGQVIQAQDSLKQKEKEEQQLQSNINELKQLTEQKKKQIETLQGEVKIAVSQKTELENKLQQQSMQAAQELAAEKQKISVLQNTYEKSQENLKQLQSDFYGKESELLATRQDLKSIEEKLSLAQEDLISNRNQIGNQNKLIQELKTTKTTLEQDLAKKEQQLKEQNKALQDMQKEKSLKEKELVNEKSKLAETEEIKCRQEKEIAKLSEELKSHKQESIKEITNLKDAKQLLIQQKLELQGKVDSLKATLEQEKKTQQMLKEQMKKEEDELKKEFMEKEAKLHSEIKEKEVGMKKHEENGAKLTMQITALNENLGTIKKEWQSSQRRVSELEKQTDDLRGEIAVLEATVQNNQDERRALLERCLKGEGEIEKLQTKVLELQRKLDNTTAAVQELGRENQSLQIKHTQALTRKWAEDSEVQNCMACGKGFSVTVRRHHCRQCGNIFCAECSAKNALTPSSKKPVRVCDGCFNDLQG

Target ORF information:

RefSeq Version	XM_005206125.4
Organism	Bos taurus(cattle)
Definition	Bos taurus early endosome antigen 1 (EEA1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005206125.4

ORF Insert Sequence:

ATGTTAAGGA GGATTCTACA GAGGACTCCT GGGAGAGTTG GCTCTCAAGG TTCAGATCTA 
GATTCATCAG CAGCTCCTAT AAACACAGTG GATGTCAATA ATGAAAGCTC TTCAGAGGGT 
TTCATCTGTC CGTTATGTAT GAAAAGATGT ATAACTGCTA TTGATTTGTC GGAGCATTAC 
CAGAAAGTGC ACGCTGAAAA TGAGACAAGA GGACAGGAAC TTCTTAATGA CTCCAGTGTT 
ACTGGTTTCA TATGTCCACA GTGTATGAAA TCTCTTGGAT CTGCTGATGA ACTTTTCAAA 
CACTATGAGG CAGTCCATGA TGCTGGTAAT GACTCAAGTC ACGGAGGAGA AGCTTTGGCT 
CTGAAGCGAG ATGATATAAC ACTACTCAGA CAAGAGGTCC AAGACCTACA GGCTTCACTT 
AAGGAAGAAA AATGGTACTC AGAAGAATTA AAGAAAGAAT TAGAAAAATT TCAAGGGCTG 
CAGCAACAAG AGTCCAAACC TGATGGATTA GTGGCTGATT CATCAGCAGA ACTACAGTCT 
TTGGAACAAC AATTAGAAGA AGCCCAAACA GAAAATTTTA ATATTAAACA AATGAAAGAT 
TTGTTTGAAC AGAAAGCAGC CCAACTTGCT ACTGAAATTG CAGATATAAA GTCAAAATAT 
GATGAAGAAA GGAGTCTTAG AGAAGCTGCT GAACAGCAAG TGACACATCT AACAGAAGAA 
TTAAACAAAG AAGCAGCTGT GATTCAAGAT CTAAAGACTG AACTGCTTCA GAGACCTGGA 
ATAGAAGATG TTGCTGTATT AAAGAAAGAA CTGGTCCAAG TTCAAACACT AATGGATAAC 
ATGACCTTGG AACGTGAGAG AGAATCTGAA AAACTCAAAG ATGAGTGCAA AAAGTTACAA 
ACAGAATATA CTAACTCAGA GGCCGTAATA AGCCAGCTGA GGAGTGAACT TGCCAAAGGC 
CCGCAGGAAG TTGCTGTGTA TGTGCAGGAA CTACAAAAAC TTAAAAGTTC AGTTAATGAA 
TTAACACAAA AAAATCAGAA CTTGACTGAA AAGTTACAGA AGAAAGAACT GGACTATACT 
CAGTTAGAAG AGAAACATAA TGAAGAATGT ATGAGTAAAA AGAGTATACA AGCAAGCCTT 
CATCAAAAAG ACCTGGATTG TCAACAGCTT CAGTCAAGGT TATCTGCATC TGAAACCTCA 
CTGCAGAGAA TACAGGCAGA ACTAGGTGAA AAGGGAGAAG CTACTCAGAA GCTCAAAGAA 
GAATTATCAG AAGTGGAGAC CAAGTACCAG CATCTTAAGG CAGAGTTTAA ACAGCTACAA 
CAACAGAGAG AAGAAAAGGA TCAGCATGGG TTACAACTGC AAAGTGAAAT CAATCAATTA 
CATAGCAAAC TTCTGGAGAC AGAGCGCCAA CTAGGAGAAG CTCATGGAAG GCTGAAGGAA 
CAGAGACAGC TTTCAAGTGA AAAGTTGATG GATAAAGAAC AACAAGTGGC TGATCTACAA 
CTCAAACTTT CTCGTTTAGA GGAAGAGTTG AAGGAAAAAG TAACCAATTC CACAGAATTG 
CAGCATCAGT TAGATAAAAC AAAGCAACAG CATCAAGAAC AACAAGCTCT TCAACAAAGC 
ACTACAGCAA AACTTCGAGA AGCTCAGAAT GATTTGGAAC AAGTACTACG TCAAATTGGT 
GATAAGGACC AAAAGATCCA GAACCTTGAA GCCTTATTAC AGAAGAGCAA AGAAAATATT 
TCTTTACTAG AAAAGGAGAG AGAAGATCTT TATGCAAAAA TTCAGGCTGG TGAGGGAGAG 
ACTGCTGTTC TTAACCAATT ACAAGAAAAA AACCATACAT TACAGGAACA AGTAACTCAG 
CTAACAGAGA AGCTGAAGAA TCAGTCAGAA AGCCATAAAC AAGCCCAGGA GAATTTGCAT 
GACCAGGTGC AGGAGCAGAA GGCACATCTT AGAGCTGCAC AAGACCGTGT CCTTTCCCTA 
GAATCCAGCA TCAATGAATT AAATAGTCAG TTAAATGAAA GCAAGGAGAA GGTTTCCCAG 
CTTGATATAC AGGTTAAAGC CAAAACTGAA TTATTGCTAT CAGCAGAAGC AGCAAAAACT 
GCTCAGAGAG CAGATCTTCA GAATCATCTG GACACAGCCC AAAATGCATT ACAAGATAAA 
CAGCAGGAGT TAAATAAGAT CACTACTCAG TTGGATCAGG TCACTACAAA GTTGCAGGAC 
AAGCAAGAAC ATTGCAGTCA GCTGGAAAGT CATCTTAAAG AATACAAAGA GAAACATCTG 
TCTTTAGAAC AGAAAACTGA AGAATTAGAA GGTCAAATCA AGAAACTAGA AGCTGATATG 
CTTGAAGTTA AAGCAAGCAA GGAGCAAGCT TTGCAGGGTC TACAACAACA GAGACAGCTG 
AACACAGATT TAGAGCTTCG AGCCACAGAA TTGAGTAAAC AACTTGAAAT GGAGAAAGAA 
ACAGTATCTA ATACAAAGTT GGATCTACAG AAGAAATCTG AAGCACTTGA AAATACTAAG 
CAAATGCTTA CCAAGCAAGA AGAAGAAAAA ACAATGCTTA AACAAGAAAT TGAAAATTTA 
AGTCAAGATG CAAAGATGCA GCACAAGGAA CTGAATGATA GGATTCAAAC AGCAGTAACA 
GAACTACAGA AAGTGAAGGT GGAGAAAGAC TCTCTGGTAG CAGAGCTTTC TGCAGCAAAA 
GAGAAACTGT CAAAAGTTTC TGATTGTTTG AAGAACTCCC AAAGTGAATT TGAAAAAGAA 
AATCAGAAGG GAAAAGCTGT TATATTAGAT TTGGAGAAAA CTTGCAAAGA ATTAAAGCAT 
CAGCTTCAAG TACAGACAGA AAGCCTGCAT AAGGAGCAGA ATGAAATGAA AAAGTCACTT 
GAAAAAGAAA AGGAGACTTC TCATCAGTTG AAGTTGGAGC TCAGTTCCAT GCAGGGACAA 
GTCATACAGG CCCAGGACAG CTTAAAACAA AAGGAAAAAG AAGAACAACA GCTTCAGAGT 
AACATAAATG AGCTAAAGCA ATTGACTGAA CAGAAGAAAA AGCAAATTGA AACACTCCAA 
GGAGAAGTTA AAATTGCTGT TTCACAGAAG ACAGAACTTG AGAATAAACT ACAGCAGCAG 
TCAATGCAAG CAGCACAGGA ACTTGCAGCA GAGAAACAAA AAATATCAGT GTTACAAAAC 
ACCTATGAAA AAAGTCAGGA AAATCTAAAA CAGCTTCAGT CGGATTTCTA TGGGAAGGAA 
TCTGAACTAC TTGCCACAAG GCAGGATCTT AAGTCTATAG AAGAGAAGCT TTCTCTAGCA 
CAGGAGGATT TGATATCAAA CAGAAATCAA ATTGGAAACC AAAATAAATT GATTCAAGAA 
CTGAAGACCA CCAAGACTAC TTTGGAGCAA GATTTAGCAA AGAAAGAACA GCAATTGAAG 
GAACAGAATA AAGCACTACA AGATATGCAA AAAGAAAAGT CACTAAAAGA AAAAGAACTA 
GTAAATGAAA AATCTAAATT GGCAGAGACA GAGGAAATTA AGTGTAGACA AGAAAAAGAA 
ATTGCTAAAT TGAGTGAAGA ACTCAAGTCT CACAAGCAGG AAAGCATAAA GGAAATAACA 
AATCTCAAGG ATGCCAAACA GCTTTTGATT CAGCAGAAAT TAGAGCTTCA AGGGAAAGTA 
GATTCCCTGA AGGCAACCCT TGAACAGGAG AAGAAAACCC AGCAAATGCT AAAAGAGCAG 
ATGAAAAAGG AAGAAGATGA GCTGAAGAAA GAGTTTATGG AAAAGGAAGC TAAACTGCAT 
TCCGAAATAA AAGAAAAAGA AGTAGGAATG AAGAAGCATG AAGAAAATGG GGCTAAACTT 
ACCATGCAGA TCACAGCATT AAATGAAAAC TTGGGCACCA TAAAGAAAGA GTGGCAGTCC 
AGTCAACGGA GAGTCAGTGA ACTCGAGAAA CAGACGGACG ACTTACGGGG TGAAATTGCA 
GTACTGGAAG CGACAGTTCA GAATAACCAA GATGAAAGGA GAGCACTGCT GGAAAGGTGT 
CTTAAAGGAG AAGGTGAAAT AGAAAAGCTT CAAACCAAAG TACTAGAATT GCAAAGAAAG 
CTGGATAATA CAACTGCAGC AGTGCAGGAG CTAGGCAGAG AGAATCAGTC CCTTCAAATC 
AAACATACAC AGGCGTTGAC TAGAAAGTGG GCTGAAGACA GTGAAGTACA AAACTGTATG 
GCCTGTGGGA AAGGCTTTTC CGTAACAGTG AGACGGCATC ACTGCAGACA ATGTGGAAAT 
ATCTTCTGTG CTGAGTGTTC AGCCAAAAAT GCCTTAACTC CTTCTTCCAA GAAGCCTGTT 
CGTGTCTGTG ATGGATGTTT CAATGACTTG CAAGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EEA1 ( XM_002687214.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002687260.2 Bos taurus early endosome antigen 1 (EEA1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa04185
Clone ID Related Accession (Same CDS sequence)	XM_002687214.5 , XM_002687214.6 , XM_594411.9
Accession Version	XM_002687214.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	early endosome antigen 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103917.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002687214.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTAAGGA GGATTCTACA GAGGACTCCT GGGAGAGTTG GCTCTCAAGG TTCAGATCTA 
GATTCATCAG CAGCTCCTAT AAACACAGTG GATGTCAATA ATGAAAGCTC TTCAGAGGGT 
TTCATATGTC CACAGTGTAT GAAATCTCTT GGATCTGCTG ATGAACTTTT CAAACACTAT 
GAGGCAGTCC ATGATGCTGG TAATGACTCA AGTCACGGAG GAGAAGCTTT GGCTCTGAAG 
CGAGATGATA TAACACTACT CAGACAAGAG GTCCAAGACC TACAGGCTTC ACTTAAGGAA 
GAAAAATGGT ACTCAGAAGA ATTAAAGAAA GAATTAGAAA AATTTCAAGG GCTGCAGCAA 
CAAGAGTCCA AACCTGATGG ATTAGTGGCT GATTCATCAG CAGAACTACA GTCTTTGGAA 
CAACAATTAG AAGAAGCCCA AACAGAAAAT TTTAATATTA AACAAATGAA AGATTTGTTT 
GAACAGAAAG CAGCCCAACT TGCTACTGAA ATTGCAGATA TAAAGTCAAA ATATGATGAA 
GAAAGGAGTC TTAGAGAAGC TGCTGAACAG CAAGTGACAC ATCTAACAGA AGAATTAAAC 
AAAGAAGCAG CTGTGATTCA AGATCTAAAG ACTGAACTGC TTCAGAGACC TGGAATAGAA 
GATGTTGCTG TATTAAAGAA AGAACTGGTC CAAGTTCAAA CACTAATGGA TAACATGACC 
TTGGAACGTG AGAGAGAATC TGAAAAACTC AAAGATGAGT GCAAAAAGTT ACAAACAGAA 
TATACTAACT CAGAGGCCGT AATAAGCCAG CTGAGGAGTG AACTTGCCAA AGGCCCGCAG 
GAAGTTGCTG TGTATGTGCA GGAACTACAA AAACTTAAAA GTTCAGTTAA TGAATTAACA 
CAAAAAAATC AGAACTTGAC TGAAAAGTTA CAGAAGAAAG AACTGGACTA TACTCAGTTA 
GAAGAGAAAC ATAATGAAGA ATGTATGAGT AAAAAGAGTA TACAAGCAAG CCTTCATCAA 
AAAGACCTGG ATTGTCAACA GCTTCAGTCA AGGTTATCTG CATCTGAAAC CTCACTGCAG 
AGAATACAGG CAGAACTAGG TGAAAAGGGA GAAGCTACTC AGAAGCTCAA AGAAGAATTA 
TCAGAAGTGG AGACCAAGTA CCAGCATCTT AAGGCAGAGT TTAAACAGCT ACAACAACAG 
AGAGAAGAAA AGGATCAGCA TGGGTTACAA CTGCAAAGTG AAATCAATCA ATTACATAGC 
AAACTTCTGG AGACAGAGCG CCAACTAGGA GAAGCTCATG GAAGGCTGAA GGAACAGAGA 
CAGCTTTCAA GTGAAAAGTT GATGGATAAA GAACAACAAG TGGCTGATCT ACAACTCAAA 
CTTTCTCGTT TAGAGGAAGA GTTGAAGGAA AAAGTAACCA ATTCCACAGA ATTGCAGCAT 
CAGTTAGATA AAACAAAGCA ACAGCATCAA GAACAACAAG CTCTTCAACA AAGCACTACA 
GCAAAACTTC GAGAAGCTCA GAATGATTTG GAACAAGTAC TACGTCAAAT TGGTGATAAG 
GACCAAAAGA TCCAGAACCT TGAAGCCTTA TTACAGAAGA GCAAAGAAAA TATTTCTTTA 
CTAGAAAAGG AGAGAGAAGA TCTTTATGCA AAAATTCAGG CTGGTGAGGG AGAGACTGCT 
GTTCTTAACC AATTACAAGA AAAAAACCAT ACATTACAGG AACAAGTAAC TCAGCTAACA 
GAGAAGCTGA AGAATCAGTC AGAAAGCCAT AAACAAGCCC AGGAGAATTT GCATGACCAG 
GTGCAGGAGC AGAAGGCACA TCTTAGAGCT GCACAAGACC GTGTCCTTTC CCTAGAATCC 
AGCATCAATG AATTAAATAG TCAGTTAAAT GAAAGCAAGG AGAAGGTTTC CCAGCTTGAT 
ATACAGGTTA AAGCCAAAAC TGAATTATTG CTATCAGCAG AAGCAGCAAA AACTGCTCAG 
AGAGCAGATC TTCAGAATCA TCTGGACACA GCCCAAAATG CATTACAAGA TAAACAGCAG 
GAGTTAAATA AGATCACTAC TCAGTTGGAT CAGGTCACTA CAAAGTTGCA GGACAAGCAA 
GAACATTGCA GTCAGCTGGA AAGTCATCTT AAAGAATACA AAGAGAAACA TCTGTCTTTA 
GAACAGAAAA CTGAAGAATT AGAAGGTCAA ATCAAGAAAC TAGAAGCTGA TATGCTTGAA 
GTTAAAGCAA GCAAGGAGCA AGCTTTGCAG GGTCTACAAC AACAGAGACA GCTGAACACA 
GATTTAGAGC TTCGAGCCAC AGAATTGAGT AAACAACTTG AAATGGAGAA AGAAACAGTA 
TCTAATACAA AGTTGGATCT ACAGAAGAAA TCTGAAGCAC TTGAAAATAC TAAGCAAATG 
CTTACCAAGC AAGAAGAAGA AAAAACAATG CTTAAACAAG AAATTGAAAA TTTAAGTCAA 
GATGCAAAGA TGCAGCACAA GGAACTGAAT GATAGGATTC AAACAGCAGT AACAGAACTA 
CAGAAAGTGA AGGTGGAGAA AGACTCTCTG GTAGCAGAGC TTTCTGCAGC AAAAGAGAAA 
CTGTCAAAAG TTTCTGATTG TTTGAAGAAC TCCCAAAGTG AATTTGAAAA AGAAAATCAG 
AAGGGAAAAG CTGTTATATT AGATTTGGAG AAAACTTGCA AAGAATTAAA GCATCAGCTT 
CAAGTACAGA CAGAAAGCCT GCATAAGGAG CAGAATGAAA TGAAAAAGTC ACTTGAAAAA 
GAAAAGGAGA CTTCTCATCA GTTGAAGTTG GAGCTCAGTT CCATGCAGGG ACAAGTCATA 
CAGGCCCAGG ACAGCTTAAA ACAAAAGGAA AAAGAAGAAC AACAGCTTCA GAGTAACATA 
AATGAGCTAA AGCAATTGAC TGAACAGAAG AAAAAGCAAA TTGAAACACT CCAAGGAGAA 
GTTAAAATTG CTGTTTCACA GAAGACAGAA CTTGAGAATA AACTACAGCA GCAGTCAATG 
CAAGCAGCAC AGGAACTTGC AGCAGAGAAA CAAAAAATAT CAGTGTTACA AAACACCTAT 
GAAAAAAGTC AGGAAAATCT AAAACAGCTT CAGTCGGATT TCTATGGGAA GGAATCTGAA 
CTACTTGCCA CAAGGCAGGA TCTTAAGTCT ATAGAAGAGA AGCTTTCTCT AGCACAGGAG 
GATTTGATAT CAAACAGAAA TCAAATTGGA AACCAAAATA AATTGATTCA AGAACTGAAG 
ACCACCAAGA CTACTTTGGA GCAAGATTTA GCAAAGAAAG AACAGCAATT GAAGGAACAG 
AATAAAGCAC TACAAGATAT GCAAAAAGAA AAGTCACTAA AAGAAAAAGA ACTAGTAAAT 
GAAAAATCTA AATTGGCAGA GACAGAGGAA ATTAAGTGTA GACAAGAAAA AGAAATTGCT 
AAATTGAGTG AAGAACTCAA GTCTCACAAG CAGGAAAGCA TAAAGGAAAT AACAAATCTC 
AAGGATGCCA AACAGCTTTT GATTCAGCAG AAATTAGAGC TTCAAGGGAA AGTAGATTCC 
CTGAAGGCAA CCCTTGAACA GGAGAAGAAA ACCCAGCAAA TGCTAAAAGA GCAGATGAAA 
AAGGAAGAAG ATGAGCTGAA GAAAGAGTTT ATGGAAAAGG AAGCTAAACT GCATTCCGAA 
ATAAAAGAAA AAGAAGTAGG AATGAAGAAG CATGAAGAAA ATGGGGCTAA ACTTACCATG 
CAGATCACAG CATTAAATGA AAACTTGGGC ACCATAAAGA AAGAGTGGCA GTCCAGTCAA 
CGGAGAGTCA GTGAACTCGA GAAACAGACG GACGACTTAC GGGGTGAAAT TGCAGTACTG 
GAAGCGACAG TTCAGAATAA CCAAGATGAA AGGAGAGCAC TGCTGGAAAG GTGTCTTAAA 
GGAGAAGGTG AAATAGAAAA GCTTCAAACC AAAGTACTAG AATTGCAAAG AAAGCTGGAT 
AATACAACTG CAGCAGTGCA GGAGCTAGGC AGAGAGAATC AGTCCCTTCA AATCAAACAT 
ACACAGGCGT TGACTAGAAA GTGGGCTGAA GACAGTGAAG TACAAAACTG TATGGCCTGT 
GGGAAAGGCT TTTCCGTAAC AGTGAGACGG CATCACTGCA GACAATGTGG AAATATCTTC 
TGTGCTGAGT GTTCAGCCAA AAATGCCTTA ACTCCTTCTT CCAAGAAGCC TGTTCGTGTC 
TGTGATGGAT GTTTCAATGA CTTGCAAGGA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002687260.2
CDS	259..4491
Translation	MLRRILQRTPGRVGSQGSDLDSSAAPINTVDVNNESSSEGFICPQCMKSLGSADELFKHYEAVHDAGNDSSHGGEALALKRDDITLLRQEVQDLQASLKEEKWYSEELKKELEKFQGLQQQESKPDGLVADSSAELQSLEQQLEEAQTENFNIKQMKDLFEQKAAQLATEIADIKSKYDEERSLREAAEQQVTHLTEELNKEAAVIQDLKTELLQRPGIEDVAVLKKELVQVQTLMDNMTLERERESEKLKDECKKLQTEYTNSEAVISQLRSELAKGPQEVAVYVQELQKLKSSVNELTQKNQNLTEKLQKKELDYTQLEEKHNEECMSKKSIQASLHQKDLDCQQLQSRLSASETSLQRIQAELGEKGEATQKLKEELSEVETKYQHLKAEFKQLQQQREEKDQHGLQLQSEINQLHSKLLETERQLGEAHGRLKEQRQLSSEKLMDKEQQVADLQLKLSRLEEELKEKVTNSTELQHQLDKTKQQHQEQQALQQSTTAKLREAQNDLEQVLRQIGDKDQKIQNLEALLQKSKENISLLEKEREDLYAKIQAGEGETAVLNQLQEKNHTLQEQVTQLTEKLKNQSESHKQAQENLHDQVQEQKAHLRAAQDRVLSLESSINELNSQLNESKEKVSQLDIQVKAKTELLLSAEAAKTAQRADLQNHLDTAQNALQDKQQELNKITTQLDQVTTKLQDKQEHCSQLESHLKEYKEKHLSLEQKTEELEGQIKKLEADMLEVKASKEQALQGLQQQRQLNTDLELRATELSKQLEMEKETVSNTKLDLQKKSEALENTKQMLTKQEEEKTMLKQEIENLSQDAKMQHKELNDRIQTAVTELQKVKVEKDSLVAELSAAKEKLSKVSDCLKNSQSEFEKENQKGKAVILDLEKTCKELKHQLQVQTESLHKEQNEMKKSLEKEKETSHQLKLELSSMQGQVIQAQDSLKQKEKEEQQLQSNINELKQLTEQKKKQIETLQGEVKIAVSQKTELENKLQQQSMQAAQELAAEKQKISVLQNTYEKSQENLKQLQSDFYGKESELLATRQDLKSIEEKLSLAQEDLISNRNQIGNQNKLIQELKTTKTTLEQDLAKKEQQLKEQNKALQDMQKEKSLKEKELVNEKSKLAETEEIKCRQEKEIAKLSEELKSHKQESIKEITNLKDAKQLLIQQKLELQGKVDSLKATLEQEKKTQQMLKEQMKKEEDELKKEFMEKEAKLHSEIKEKEVGMKKHEENGAKLTMQITALNENLGTIKKEWQSSQRRVSELEKQTDDLRGEIAVLEATVQNNQDERRALLERCLKGEGEIEKLQTKVLELQRKLDNTTAAVQELGRENQSLQIKHTQALTRKWAEDSEVQNCMACGKGFSVTVRRHHCRQCGNIFCAECSAKNALTPSSKKPVRVCDGCFNDLQG

Target ORF information:

RefSeq Version	XM_002687214.5
Organism	Bos taurus(cattle)
Definition	Bos taurus early endosome antigen 1 (EEA1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002687214.5

ORF Insert Sequence:

ATGTTAAGGA GGATTCTACA GAGGACTCCT GGGAGAGTTG GCTCTCAAGG TTCAGATCTA 
GATTCATCAG CAGCTCCTAT AAACACAGTG GATGTCAATA ATGAAAGCTC TTCAGAGGGT 
TTCATATGTC CACAGTGTAT GAAATCTCTT GGATCTGCTG ATGAACTTTT CAAACACTAT 
GAGGCAGTCC ATGATGCTGG TAATGACTCA AGTCACGGAG GAGAAGCTTT GGCTCTGAAG 
CGAGATGATA TAACACTACT CAGACAAGAG GTCCAAGACC TACAGGCTTC ACTTAAGGAA 
GAAAAATGGT ACTCAGAAGA ATTAAAGAAA GAATTAGAAA AATTTCAAGG GCTGCAGCAA 
CAAGAGTCCA AACCTGATGG ATTAGTGGCT GATTCATCAG CAGAACTACA GTCTTTGGAA 
CAACAATTAG AAGAAGCCCA AACAGAAAAT TTTAATATTA AACAAATGAA AGATTTGTTT 
GAACAGAAAG CAGCCCAACT TGCTACTGAA ATTGCAGATA TAAAGTCAAA ATATGATGAA 
GAAAGGAGTC TTAGAGAAGC TGCTGAACAG CAAGTGACAC ATCTAACAGA AGAATTAAAC 
AAAGAAGCAG CTGTGATTCA AGATCTAAAG ACTGAACTGC TTCAGAGACC TGGAATAGAA 
GATGTTGCTG TATTAAAGAA AGAACTGGTC CAAGTTCAAA CACTAATGGA TAACATGACC 
TTGGAACGTG AGAGAGAATC TGAAAAACTC AAAGATGAGT GCAAAAAGTT ACAAACAGAA 
TATACTAACT CAGAGGCCGT AATAAGCCAG CTGAGGAGTG AACTTGCCAA AGGCCCGCAG 
GAAGTTGCTG TGTATGTGCA GGAACTACAA AAACTTAAAA GTTCAGTTAA TGAATTAACA 
CAAAAAAATC AGAACTTGAC TGAAAAGTTA CAGAAGAAAG AACTGGACTA TACTCAGTTA 
GAAGAGAAAC ATAATGAAGA ATGTATGAGT AAAAAGAGTA TACAAGCAAG CCTTCATCAA 
AAAGACCTGG ATTGTCAACA GCTTCAGTCA AGGTTATCTG CATCTGAAAC CTCACTGCAG 
AGAATACAGG CAGAACTAGG TGAAAAGGGA GAAGCTACTC AGAAGCTCAA AGAAGAATTA 
TCAGAAGTGG AGACCAAGTA CCAGCATCTT AAGGCAGAGT TTAAACAGCT ACAACAACAG 
AGAGAAGAAA AGGATCAGCA TGGGTTACAA CTGCAAAGTG AAATCAATCA ATTACATAGC 
AAACTTCTGG AGACAGAGCG CCAACTAGGA GAAGCTCATG GAAGGCTGAA GGAACAGAGA 
CAGCTTTCAA GTGAAAAGTT GATGGATAAA GAACAACAAG TGGCTGATCT ACAACTCAAA 
CTTTCTCGTT TAGAGGAAGA GTTGAAGGAA AAAGTAACCA ATTCCACAGA ATTGCAGCAT 
CAGTTAGATA AAACAAAGCA ACAGCATCAA GAACAACAAG CTCTTCAACA AAGCACTACA 
GCAAAACTTC GAGAAGCTCA GAATGATTTG GAACAAGTAC TACGTCAAAT TGGTGATAAG 
GACCAAAAGA TCCAGAACCT TGAAGCCTTA TTACAGAAGA GCAAAGAAAA TATTTCTTTA 
CTAGAAAAGG AGAGAGAAGA TCTTTATGCA AAAATTCAGG CTGGTGAGGG AGAGACTGCT 
GTTCTTAACC AATTACAAGA AAAAAACCAT ACATTACAGG AACAAGTAAC TCAGCTAACA 
GAGAAGCTGA AGAATCAGTC AGAAAGCCAT AAACAAGCCC AGGAGAATTT GCATGACCAG 
GTGCAGGAGC AGAAGGCACA TCTTAGAGCT GCACAAGACC GTGTCCTTTC CCTAGAATCC 
AGCATCAATG AATTAAATAG TCAGTTAAAT GAAAGCAAGG AGAAGGTTTC CCAGCTTGAT 
ATACAGGTTA AAGCCAAAAC TGAATTATTG CTATCAGCAG AAGCAGCAAA AACTGCTCAG 
AGAGCAGATC TTCAGAATCA TCTGGACACA GCCCAAAATG CATTACAAGA TAAACAGCAG 
GAGTTAAATA AGATCACTAC TCAGTTGGAT CAGGTCACTA CAAAGTTGCA GGACAAGCAA 
GAACATTGCA GTCAGCTGGA AAGTCATCTT AAAGAATACA AAGAGAAACA TCTGTCTTTA 
GAACAGAAAA CTGAAGAATT AGAAGGTCAA ATCAAGAAAC TAGAAGCTGA TATGCTTGAA 
GTTAAAGCAA GCAAGGAGCA AGCTTTGCAG GGTCTACAAC AACAGAGACA GCTGAACACA 
GATTTAGAGC TTCGAGCCAC AGAATTGAGT AAACAACTTG AAATGGAGAA AGAAACAGTA 
TCTAATACAA AGTTGGATCT ACAGAAGAAA TCTGAAGCAC TTGAAAATAC TAAGCAAATG 
CTTACCAAGC AAGAAGAAGA AAAAACAATG CTTAAACAAG AAATTGAAAA TTTAAGTCAA 
GATGCAAAGA TGCAGCACAA GGAACTGAAT GATAGGATTC AAACAGCAGT AACAGAACTA 
CAGAAAGTGA AGGTGGAGAA AGACTCTCTG GTAGCAGAGC TTTCTGCAGC AAAAGAGAAA 
CTGTCAAAAG TTTCTGATTG TTTGAAGAAC TCCCAAAGTG AATTTGAAAA AGAAAATCAG 
AAGGGAAAAG CTGTTATATT AGATTTGGAG AAAACTTGCA AAGAATTAAA GCATCAGCTT 
CAAGTACAGA CAGAAAGCCT GCATAAGGAG CAGAATGAAA TGAAAAAGTC ACTTGAAAAA 
GAAAAGGAGA CTTCTCATCA GTTGAAGTTG GAGCTCAGTT CCATGCAGGG ACAAGTCATA 
CAGGCCCAGG ACAGCTTAAA ACAAAAGGAA AAAGAAGAAC AACAGCTTCA GAGTAACATA 
AATGAGCTAA AGCAATTGAC TGAACAGAAG AAAAAGCAAA TTGAAACACT CCAAGGAGAA 
GTTAAAATTG CTGTTTCACA GAAGACAGAA CTTGAGAATA AACTACAGCA GCAGTCAATG 
CAAGCAGCAC AGGAACTTGC AGCAGAGAAA CAAAAAATAT CAGTGTTACA AAACACCTAT 
GAAAAAAGTC AGGAAAATCT AAAACAGCTT CAGTCGGATT TCTATGGGAA GGAATCTGAA 
CTACTTGCCA CAAGGCAGGA TCTTAAGTCT ATAGAAGAGA AGCTTTCTCT AGCACAGGAG 
GATTTGATAT CAAACAGAAA TCAAATTGGA AACCAAAATA AATTGATTCA AGAACTGAAG 
ACCACCAAGA CTACTTTGGA GCAAGATTTA GCAAAGAAAG AACAGCAATT GAAGGAACAG 
AATAAAGCAC TACAAGATAT GCAAAAAGAA AAGTCACTAA AAGAAAAAGA ACTAGTAAAT 
GAAAAATCTA AATTGGCAGA GACAGAGGAA ATTAAGTGTA GACAAGAAAA AGAAATTGCT 
AAATTGAGTG AAGAACTCAA GTCTCACAAG CAGGAAAGCA TAAAGGAAAT AACAAATCTC 
AAGGATGCCA AACAGCTTTT GATTCAGCAG AAATTAGAGC TTCAAGGGAA AGTAGATTCC 
CTGAAGGCAA CCCTTGAACA GGAGAAGAAA ACCCAGCAAA TGCTAAAAGA GCAGATGAAA 
AAGGAAGAAG ATGAGCTGAA GAAAGAGTTT ATGGAAAAGG AAGCTAAACT GCATTCCGAA 
ATAAAAGAAA AAGAAGTAGG AATGAAGAAG CATGAAGAAA ATGGGGCTAA ACTTACCATG 
CAGATCACAG CATTAAATGA AAACTTGGGC ACCATAAAGA AAGAGTGGCA GTCCAGTCAA 
CGGAGAGTCA GTGAACTCGA GAAACAGACG GACGACTTAC GGGGTGAAAT TGCAGTACTG 
GAAGCGACAG TTCAGAATAA CCAAGATGAA AGGAGAGCAC TGCTGGAAAG GTGTCTTAAA 
GGAGAAGGTG AAATAGAAAA GCTTCAAACC AAAGTACTAG AATTGCAAAG AAAGCTGGAT 
AATACAACTG CAGCAGTGCA GGAGCTAGGC AGAGAGAATC AGTCCCTTCA AATCAAACAT 
ACACAGGCGT TGACTAGAAA GTGGGCTGAA GACAGTGAAG TACAAAACTG TATGGCCTGT 
GGGAAAGGCT TTTCCGTAAC AGTGAGACGG CATCACTGCA GACAATGTGG AAATATCTTC 
TGTGCTGAGT GTTCAGCCAA AAATGCCTTA ACTCCTTCTT CCAAGAAGCC TGTTCGTGTC 
TGTGATGGAT GTTTCAATGA CTTGCAAGGA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EEA1 ( XM_002687214.6 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002687260.2 Bos taurus early endosome antigen 1 (EEA1), transcript variant X4, mRNA.

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CloneID	OBa04185
Clone ID Related Accession (Same CDS sequence)	XM_002687214.5 , XM_002687214.6 , XM_594411.9
Accession Version	XM_002687214.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	early endosome antigen 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002687214.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTAAGGA GGATTCTACA GAGGACTCCT GGGAGAGTTG GCTCTCAAGG TTCAGATCTA 
GATTCATCAG CAGCTCCTAT AAACACAGTG GATGTCAATA ATGAAAGCTC TTCAGAGGGT 
TTCATATGTC CACAGTGTAT GAAATCTCTT GGATCTGCTG ATGAACTTTT CAAACACTAT 
GAGGCAGTCC ATGATGCTGG TAATGACTCA AGTCACGGAG GAGAAGCTTT GGCTCTGAAG 
CGAGATGATA TAACACTACT CAGACAAGAG GTCCAAGACC TACAGGCTTC ACTTAAGGAA 
GAAAAATGGT ACTCAGAAGA ATTAAAGAAA GAATTAGAAA AATTTCAAGG GCTGCAGCAA 
CAAGAGTCCA AACCTGATGG ATTAGTGGCT GATTCATCAG CAGAACTACA GTCTTTGGAA 
CAACAATTAG AAGAAGCCCA AACAGAAAAT TTTAATATTA AACAAATGAA AGATTTGTTT 
GAACAGAAAG CAGCCCAACT TGCTACTGAA ATTGCAGATA TAAAGTCAAA ATATGATGAA 
GAAAGGAGTC TTAGAGAAGC TGCTGAACAG CAAGTGACAC ATCTAACAGA AGAATTAAAC 
AAAGAAGCAG CTGTGATTCA AGATCTAAAG ACTGAACTGC TTCAGAGACC TGGAATAGAA 
GATGTTGCTG TATTAAAGAA AGAACTGGTC CAAGTTCAAA CACTAATGGA TAACATGACC 
TTGGAACGTG AGAGAGAATC TGAAAAACTC AAAGATGAGT GCAAAAAGTT ACAAACAGAA 
TATACTAACT CAGAGGCCGT AATAAGCCAG CTGAGGAGTG AACTTGCCAA AGGCCCGCAG 
GAAGTTGCTG TGTATGTGCA GGAACTACAA AAACTTAAAA GTTCAGTTAA TGAATTAACA 
CAAAAAAATC AGAACTTGAC TGAAAAGTTA CAGAAGAAAG AACTGGACTA TACTCAGTTA 
GAAGAGAAAC ATAATGAAGA ATGTATGAGT AAAAAGAGTA TACAAGCAAG CCTTCATCAA 
AAAGACCTGG ATTGTCAACA GCTTCAGTCA AGGTTATCTG CATCTGAAAC CTCACTGCAG 
AGAATACAGG CAGAACTAGG TGAAAAGGGA GAAGCTACTC AGAAGCTCAA AGAAGAATTA 
TCAGAAGTGG AGACCAAGTA CCAGCATCTT AAGGCAGAGT TTAAACAGCT ACAACAACAG 
AGAGAAGAAA AGGATCAGCA TGGGTTACAA CTGCAAAGTG AAATCAATCA ATTACATAGC 
AAACTTCTGG AGACAGAGCG CCAACTAGGA GAAGCTCATG GAAGGCTGAA GGAACAGAGA 
CAGCTTTCAA GTGAAAAGTT GATGGATAAA GAACAACAAG TGGCTGATCT ACAACTCAAA 
CTTTCTCGTT TAGAGGAAGA GTTGAAGGAA AAAGTAACCA ATTCCACAGA ATTGCAGCAT 
CAGTTAGATA AAACAAAGCA ACAGCATCAA GAACAACAAG CTCTTCAACA AAGCACTACA 
GCAAAACTTC GAGAAGCTCA GAATGATTTG GAACAAGTAC TACGTCAAAT TGGTGATAAG 
GACCAAAAGA TCCAGAACCT TGAAGCCTTA TTACAGAAGA GCAAAGAAAA TATTTCTTTA 
CTAGAAAAGG AGAGAGAAGA TCTTTATGCA AAAATTCAGG CTGGTGAGGG AGAGACTGCT 
GTTCTTAACC AATTACAAGA AAAAAACCAT ACATTACAGG AACAAGTAAC TCAGCTAACA 
GAGAAGCTGA AGAATCAGTC AGAAAGCCAT AAACAAGCCC AGGAGAATTT GCATGACCAG 
GTGCAGGAGC AGAAGGCACA TCTTAGAGCT GCACAAGACC GTGTCCTTTC CCTAGAATCC 
AGCATCAATG AATTAAATAG TCAGTTAAAT GAAAGCAAGG AGAAGGTTTC CCAGCTTGAT 
ATACAGGTTA AAGCCAAAAC TGAATTATTG CTATCAGCAG AAGCAGCAAA AACTGCTCAG 
AGAGCAGATC TTCAGAATCA TCTGGACACA GCCCAAAATG CATTACAAGA TAAACAGCAG 
GAGTTAAATA AGATCACTAC TCAGTTGGAT CAGGTCACTA CAAAGTTGCA GGACAAGCAA 
GAACATTGCA GTCAGCTGGA AAGTCATCTT AAAGAATACA AAGAGAAACA TCTGTCTTTA 
GAACAGAAAA CTGAAGAATT AGAAGGTCAA ATCAAGAAAC TAGAAGCTGA TATGCTTGAA 
GTTAAAGCAA GCAAGGAGCA AGCTTTGCAG GGTCTACAAC AACAGAGACA GCTGAACACA 
GATTTAGAGC TTCGAGCCAC AGAATTGAGT AAACAACTTG AAATGGAGAA AGAAACAGTA 
TCTAATACAA AGTTGGATCT ACAGAAGAAA TCTGAAGCAC TTGAAAATAC TAAGCAAATG 
CTTACCAAGC AAGAAGAAGA AAAAACAATG CTTAAACAAG AAATTGAAAA TTTAAGTCAA 
GATGCAAAGA TGCAGCACAA GGAACTGAAT GATAGGATTC AAACAGCAGT AACAGAACTA 
CAGAAAGTGA AGGTGGAGAA AGACTCTCTG GTAGCAGAGC TTTCTGCAGC AAAAGAGAAA 
CTGTCAAAAG TTTCTGATTG TTTGAAGAAC TCCCAAAGTG AATTTGAAAA AGAAAATCAG 
AAGGGAAAAG CTGTTATATT AGATTTGGAG AAAACTTGCA AAGAATTAAA GCATCAGCTT 
CAAGTACAGA CAGAAAGCCT GCATAAGGAG CAGAATGAAA TGAAAAAGTC ACTTGAAAAA 
GAAAAGGAGA CTTCTCATCA GTTGAAGTTG GAGCTCAGTT CCATGCAGGG ACAAGTCATA 
CAGGCCCAGG ACAGCTTAAA ACAAAAGGAA AAAGAAGAAC AACAGCTTCA GAGTAACATA 
AATGAGCTAA AGCAATTGAC TGAACAGAAG AAAAAGCAAA TTGAAACACT CCAAGGAGAA 
GTTAAAATTG CTGTTTCACA GAAGACAGAA CTTGAGAATA AACTACAGCA GCAGTCAATG 
CAAGCAGCAC AGGAACTTGC AGCAGAGAAA CAAAAAATAT CAGTGTTACA AAACACCTAT 
GAAAAAAGTC AGGAAAATCT AAAACAGCTT CAGTCGGATT TCTATGGGAA GGAATCTGAA 
CTACTTGCCA CAAGGCAGGA TCTTAAGTCT ATAGAAGAGA AGCTTTCTCT AGCACAGGAG 
GATTTGATAT CAAACAGAAA TCAAATTGGA AACCAAAATA AATTGATTCA AGAACTGAAG 
ACCACCAAGA CTACTTTGGA GCAAGATTTA GCAAAGAAAG AACAGCAATT GAAGGAACAG 
AATAAAGCAC TACAAGATAT GCAAAAAGAA AAGTCACTAA AAGAAAAAGA ACTAGTAAAT 
GAAAAATCTA AATTGGCAGA GACAGAGGAA ATTAAGTGTA GACAAGAAAA AGAAATTGCT 
AAATTGAGTG AAGAACTCAA GTCTCACAAG CAGGAAAGCA TAAAGGAAAT AACAAATCTC 
AAGGATGCCA AACAGCTTTT GATTCAGCAG AAATTAGAGC TTCAAGGGAA AGTAGATTCC 
CTGAAGGCAA CCCTTGAACA GGAGAAGAAA ACCCAGCAAA TGCTAAAAGA GCAGATGAAA 
AAGGAAGAAG ATGAGCTGAA GAAAGAGTTT ATGGAAAAGG AAGCTAAACT GCATTCCGAA 
ATAAAAGAAA AAGAAGTAGG AATGAAGAAG CATGAAGAAA ATGGGGCTAA ACTTACCATG 
CAGATCACAG CATTAAATGA AAACTTGGGC ACCATAAAGA AAGAGTGGCA GTCCAGTCAA 
CGGAGAGTCA GTGAACTCGA GAAACAGACG GACGACTTAC GGGGTGAAAT TGCAGTACTG 
GAAGCGACAG TTCAGAATAA CCAAGATGAA AGGAGAGCAC TGCTGGAAAG GTGTCTTAAA 
GGAGAAGGTG AAATAGAAAA GCTTCAAACC AAAGTACTAG AATTGCAAAG AAAGCTGGAT 
AATACAACTG CAGCAGTGCA GGAGCTAGGC AGAGAGAATC AGTCCCTTCA AATCAAACAT 
ACACAGGCGT TGACTAGAAA GTGGGCTGAA GACAGTGAAG TACAAAACTG TATGGCCTGT 
GGGAAAGGCT TTTCCGTAAC AGTGAGACGG CATCACTGCA GACAATGTGG AAATATCTTC 
TGTGCTGAGT GTTCAGCCAA AAATGCCTTA ACTCCTTCTT CCAAGAAGCC TGTTCGTGTC 
TGTGATGGAT GTTTCAATGA CTTGCAAGGA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002687260.2
CDS	293..4525
Translation	MLRRILQRTPGRVGSQGSDLDSSAAPINTVDVNNESSSEGFICPQCMKSLGSADELFKHYEAVHDAGNDSSHGGEALALKRDDITLLRQEVQDLQASLKEEKWYSEELKKELEKFQGLQQQESKPDGLVADSSAELQSLEQQLEEAQTENFNIKQMKDLFEQKAAQLATEIADIKSKYDEERSLREAAEQQVTHLTEELNKEAAVIQDLKTELLQRPGIEDVAVLKKELVQVQTLMDNMTLERERESEKLKDECKKLQTEYTNSEAVISQLRSELAKGPQEVAVYVQELQKLKSSVNELTQKNQNLTEKLQKKELDYTQLEEKHNEECMSKKSIQASLHQKDLDCQQLQSRLSASETSLQRIQAELGEKGEATQKLKEELSEVETKYQHLKAEFKQLQQQREEKDQHGLQLQSEINQLHSKLLETERQLGEAHGRLKEQRQLSSEKLMDKEQQVADLQLKLSRLEEELKEKVTNSTELQHQLDKTKQQHQEQQALQQSTTAKLREAQNDLEQVLRQIGDKDQKIQNLEALLQKSKENISLLEKEREDLYAKIQAGEGETAVLNQLQEKNHTLQEQVTQLTEKLKNQSESHKQAQENLHDQVQEQKAHLRAAQDRVLSLESSINELNSQLNESKEKVSQLDIQVKAKTELLLSAEAAKTAQRADLQNHLDTAQNALQDKQQELNKITTQLDQVTTKLQDKQEHCSQLESHLKEYKEKHLSLEQKTEELEGQIKKLEADMLEVKASKEQALQGLQQQRQLNTDLELRATELSKQLEMEKETVSNTKLDLQKKSEALENTKQMLTKQEEEKTMLKQEIENLSQDAKMQHKELNDRIQTAVTELQKVKVEKDSLVAELSAAKEKLSKVSDCLKNSQSEFEKENQKGKAVILDLEKTCKELKHQLQVQTESLHKEQNEMKKSLEKEKETSHQLKLELSSMQGQVIQAQDSLKQKEKEEQQLQSNINELKQLTEQKKKQIETLQGEVKIAVSQKTELENKLQQQSMQAAQELAAEKQKISVLQNTYEKSQENLKQLQSDFYGKESELLATRQDLKSIEEKLSLAQEDLISNRNQIGNQNKLIQELKTTKTTLEQDLAKKEQQLKEQNKALQDMQKEKSLKEKELVNEKSKLAETEEIKCRQEKEIAKLSEELKSHKQESIKEITNLKDAKQLLIQQKLELQGKVDSLKATLEQEKKTQQMLKEQMKKEEDELKKEFMEKEAKLHSEIKEKEVGMKKHEENGAKLTMQITALNENLGTIKKEWQSSQRRVSELEKQTDDLRGEIAVLEATVQNNQDERRALLERCLKGEGEIEKLQTKVLELQRKLDNTTAAVQELGRENQSLQIKHTQALTRKWAEDSEVQNCMACGKGFSVTVRRHHCRQCGNIFCAECSAKNALTPSSKKPVRVCDGCFNDLQG

Target ORF information:

RefSeq Version	XM_002687214.6
Organism	Bos taurus(cattle)
Definition	Bos taurus early endosome antigen 1 (EEA1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002687214.6

ORF Insert Sequence:

ATGTTAAGGA GGATTCTACA GAGGACTCCT GGGAGAGTTG GCTCTCAAGG TTCAGATCTA 
GATTCATCAG CAGCTCCTAT AAACACAGTG GATGTCAATA ATGAAAGCTC TTCAGAGGGT 
TTCATATGTC CACAGTGTAT GAAATCTCTT GGATCTGCTG ATGAACTTTT CAAACACTAT 
GAGGCAGTCC ATGATGCTGG TAATGACTCA AGTCACGGAG GAGAAGCTTT GGCTCTGAAG 
CGAGATGATA TAACACTACT CAGACAAGAG GTCCAAGACC TACAGGCTTC ACTTAAGGAA 
GAAAAATGGT ACTCAGAAGA ATTAAAGAAA GAATTAGAAA AATTTCAAGG GCTGCAGCAA 
CAAGAGTCCA AACCTGATGG ATTAGTGGCT GATTCATCAG CAGAACTACA GTCTTTGGAA 
CAACAATTAG AAGAAGCCCA AACAGAAAAT TTTAATATTA AACAAATGAA AGATTTGTTT 
GAACAGAAAG CAGCCCAACT TGCTACTGAA ATTGCAGATA TAAAGTCAAA ATATGATGAA 
GAAAGGAGTC TTAGAGAAGC TGCTGAACAG CAAGTGACAC ATCTAACAGA AGAATTAAAC 
AAAGAAGCAG CTGTGATTCA AGATCTAAAG ACTGAACTGC TTCAGAGACC TGGAATAGAA 
GATGTTGCTG TATTAAAGAA AGAACTGGTC CAAGTTCAAA CACTAATGGA TAACATGACC 
TTGGAACGTG AGAGAGAATC TGAAAAACTC AAAGATGAGT GCAAAAAGTT ACAAACAGAA 
TATACTAACT CAGAGGCCGT AATAAGCCAG CTGAGGAGTG AACTTGCCAA AGGCCCGCAG 
GAAGTTGCTG TGTATGTGCA GGAACTACAA AAACTTAAAA GTTCAGTTAA TGAATTAACA 
CAAAAAAATC AGAACTTGAC TGAAAAGTTA CAGAAGAAAG AACTGGACTA TACTCAGTTA 
GAAGAGAAAC ATAATGAAGA ATGTATGAGT AAAAAGAGTA TACAAGCAAG CCTTCATCAA 
AAAGACCTGG ATTGTCAACA GCTTCAGTCA AGGTTATCTG CATCTGAAAC CTCACTGCAG 
AGAATACAGG CAGAACTAGG TGAAAAGGGA GAAGCTACTC AGAAGCTCAA AGAAGAATTA 
TCAGAAGTGG AGACCAAGTA CCAGCATCTT AAGGCAGAGT TTAAACAGCT ACAACAACAG 
AGAGAAGAAA AGGATCAGCA TGGGTTACAA CTGCAAAGTG AAATCAATCA ATTACATAGC 
AAACTTCTGG AGACAGAGCG CCAACTAGGA GAAGCTCATG GAAGGCTGAA GGAACAGAGA 
CAGCTTTCAA GTGAAAAGTT GATGGATAAA GAACAACAAG TGGCTGATCT ACAACTCAAA 
CTTTCTCGTT TAGAGGAAGA GTTGAAGGAA AAAGTAACCA ATTCCACAGA ATTGCAGCAT 
CAGTTAGATA AAACAAAGCA ACAGCATCAA GAACAACAAG CTCTTCAACA AAGCACTACA 
GCAAAACTTC GAGAAGCTCA GAATGATTTG GAACAAGTAC TACGTCAAAT TGGTGATAAG 
GACCAAAAGA TCCAGAACCT TGAAGCCTTA TTACAGAAGA GCAAAGAAAA TATTTCTTTA 
CTAGAAAAGG AGAGAGAAGA TCTTTATGCA AAAATTCAGG CTGGTGAGGG AGAGACTGCT 
GTTCTTAACC AATTACAAGA AAAAAACCAT ACATTACAGG AACAAGTAAC TCAGCTAACA 
GAGAAGCTGA AGAATCAGTC AGAAAGCCAT AAACAAGCCC AGGAGAATTT GCATGACCAG 
GTGCAGGAGC AGAAGGCACA TCTTAGAGCT GCACAAGACC GTGTCCTTTC CCTAGAATCC 
AGCATCAATG AATTAAATAG TCAGTTAAAT GAAAGCAAGG AGAAGGTTTC CCAGCTTGAT 
ATACAGGTTA AAGCCAAAAC TGAATTATTG CTATCAGCAG AAGCAGCAAA AACTGCTCAG 
AGAGCAGATC TTCAGAATCA TCTGGACACA GCCCAAAATG CATTACAAGA TAAACAGCAG 
GAGTTAAATA AGATCACTAC TCAGTTGGAT CAGGTCACTA CAAAGTTGCA GGACAAGCAA 
GAACATTGCA GTCAGCTGGA AAGTCATCTT AAAGAATACA AAGAGAAACA TCTGTCTTTA 
GAACAGAAAA CTGAAGAATT AGAAGGTCAA ATCAAGAAAC TAGAAGCTGA TATGCTTGAA 
GTTAAAGCAA GCAAGGAGCA AGCTTTGCAG GGTCTACAAC AACAGAGACA GCTGAACACA 
GATTTAGAGC TTCGAGCCAC AGAATTGAGT AAACAACTTG AAATGGAGAA AGAAACAGTA 
TCTAATACAA AGTTGGATCT ACAGAAGAAA TCTGAAGCAC TTGAAAATAC TAAGCAAATG 
CTTACCAAGC AAGAAGAAGA AAAAACAATG CTTAAACAAG AAATTGAAAA TTTAAGTCAA 
GATGCAAAGA TGCAGCACAA GGAACTGAAT GATAGGATTC AAACAGCAGT AACAGAACTA 
CAGAAAGTGA AGGTGGAGAA AGACTCTCTG GTAGCAGAGC TTTCTGCAGC AAAAGAGAAA 
CTGTCAAAAG TTTCTGATTG TTTGAAGAAC TCCCAAAGTG AATTTGAAAA AGAAAATCAG 
AAGGGAAAAG CTGTTATATT AGATTTGGAG AAAACTTGCA AAGAATTAAA GCATCAGCTT 
CAAGTACAGA CAGAAAGCCT GCATAAGGAG CAGAATGAAA TGAAAAAGTC ACTTGAAAAA 
GAAAAGGAGA CTTCTCATCA GTTGAAGTTG GAGCTCAGTT CCATGCAGGG ACAAGTCATA 
CAGGCCCAGG ACAGCTTAAA ACAAAAGGAA AAAGAAGAAC AACAGCTTCA GAGTAACATA 
AATGAGCTAA AGCAATTGAC TGAACAGAAG AAAAAGCAAA TTGAAACACT CCAAGGAGAA 
GTTAAAATTG CTGTTTCACA GAAGACAGAA CTTGAGAATA AACTACAGCA GCAGTCAATG 
CAAGCAGCAC AGGAACTTGC AGCAGAGAAA CAAAAAATAT CAGTGTTACA AAACACCTAT 
GAAAAAAGTC AGGAAAATCT AAAACAGCTT CAGTCGGATT TCTATGGGAA GGAATCTGAA 
CTACTTGCCA CAAGGCAGGA TCTTAAGTCT ATAGAAGAGA AGCTTTCTCT AGCACAGGAG 
GATTTGATAT CAAACAGAAA TCAAATTGGA AACCAAAATA AATTGATTCA AGAACTGAAG 
ACCACCAAGA CTACTTTGGA GCAAGATTTA GCAAAGAAAG AACAGCAATT GAAGGAACAG 
AATAAAGCAC TACAAGATAT GCAAAAAGAA AAGTCACTAA AAGAAAAAGA ACTAGTAAAT 
GAAAAATCTA AATTGGCAGA GACAGAGGAA ATTAAGTGTA GACAAGAAAA AGAAATTGCT 
AAATTGAGTG AAGAACTCAA GTCTCACAAG CAGGAAAGCA TAAAGGAAAT AACAAATCTC 
AAGGATGCCA AACAGCTTTT GATTCAGCAG AAATTAGAGC TTCAAGGGAA AGTAGATTCC 
CTGAAGGCAA CCCTTGAACA GGAGAAGAAA ACCCAGCAAA TGCTAAAAGA GCAGATGAAA 
AAGGAAGAAG ATGAGCTGAA GAAAGAGTTT ATGGAAAAGG AAGCTAAACT GCATTCCGAA 
ATAAAAGAAA AAGAAGTAGG AATGAAGAAG CATGAAGAAA ATGGGGCTAA ACTTACCATG 
CAGATCACAG CATTAAATGA AAACTTGGGC ACCATAAAGA AAGAGTGGCA GTCCAGTCAA 
CGGAGAGTCA GTGAACTCGA GAAACAGACG GACGACTTAC GGGGTGAAAT TGCAGTACTG 
GAAGCGACAG TTCAGAATAA CCAAGATGAA AGGAGAGCAC TGCTGGAAAG GTGTCTTAAA 
GGAGAAGGTG AAATAGAAAA GCTTCAAACC AAAGTACTAG AATTGCAAAG AAAGCTGGAT 
AATACAACTG CAGCAGTGCA GGAGCTAGGC AGAGAGAATC AGTCCCTTCA AATCAAACAT 
ACACAGGCGT TGACTAGAAA GTGGGCTGAA GACAGTGAAG TACAAAACTG TATGGCCTGT 
GGGAAAGGCT TTTCCGTAAC AGTGAGACGG CATCACTGCA GACAATGTGG AAATATCTTC 
TGTGCTGAGT GTTCAGCCAA AAATGCCTTA ACTCCTTCTT CCAAGAAGCC TGTTCGTGTC 
TGTGATGGAT GTTTCAATGA CTTGCAAGGA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EEA1 ( XM_594411.9 ) cDNA ORF clone, Bos taurus(cattle) -> XP_594411.6 Bos taurus early endosome antigen 1 (EEA1), transcript variant X2, mRNA.

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CloneID	OBa04185
Clone ID Related Accession (Same CDS sequence)	XM_002687214.5 , XM_002687214.6 , XM_594411.9
Accession Version	XM_594411.9 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	early endosome antigen 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644784.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_594411.8. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTAAGGA GGATTCTACA GAGGACTCCT GGGAGAGTTG GCTCTCAAGG TTCAGATCTA 
GATTCATCAG CAGCTCCTAT AAACACAGTG GATGTCAATA ATGAAAGCTC TTCAGAGGGT 
TTCATATGTC CACAGTGTAT GAAATCTCTT GGATCTGCTG ATGAACTTTT CAAACACTAT 
GAGGCAGTCC ATGATGCTGG TAATGACTCA AGTCACGGAG GAGAAGCTTT GGCTCTGAAG 
CGAGATGATA TAACACTACT CAGACAAGAG GTCCAAGACC TACAGGCTTC ACTTAAGGAA 
GAAAAATGGT ACTCAGAAGA ATTAAAGAAA GAATTAGAAA AATTTCAAGG GCTGCAGCAA 
CAAGAGTCCA AACCTGATGG ATTAGTGGCT GATTCATCAG CAGAACTACA GTCTTTGGAA 
CAACAATTAG AAGAAGCCCA AACAGAAAAT TTTAATATTA AACAAATGAA AGATTTGTTT 
GAACAGAAAG CAGCCCAACT TGCTACTGAA ATTGCAGATA TAAAGTCAAA ATATGATGAA 
GAAAGGAGTC TTAGAGAAGC TGCTGAACAG CAAGTGACAC ATCTAACAGA AGAATTAAAC 
AAAGAAGCAG CTGTGATTCA AGATCTAAAG ACTGAACTGC TTCAGAGACC TGGAATAGAA 
GATGTTGCTG TATTAAAGAA AGAACTGGTC CAAGTTCAAA CACTAATGGA TAACATGACC 
TTGGAACGTG AGAGAGAATC TGAAAAACTC AAAGATGAGT GCAAAAAGTT ACAAACAGAA 
TATACTAACT CAGAGGCCGT AATAAGCCAG CTGAGGAGTG AACTTGCCAA AGGCCCGCAG 
GAAGTTGCTG TGTATGTGCA GGAACTACAA AAACTTAAAA GTTCAGTTAA TGAATTAACA 
CAAAAAAATC AGAACTTGAC TGAAAAGTTA CAGAAGAAAG AACTGGACTA TACTCAGTTA 
GAAGAGAAAC ATAATGAAGA ATGTATGAGT AAAAAGAGTA TACAAGCAAG CCTTCATCAA 
AAAGACCTGG ATTGTCAACA GCTTCAGTCA AGGTTATCTG CATCTGAAAC CTCACTGCAG 
AGAATACAGG CAGAACTAGG TGAAAAGGGA GAAGCTACTC AGAAGCTCAA AGAAGAATTA 
TCAGAAGTGG AGACCAAGTA CCAGCATCTT AAGGCAGAGT TTAAACAGCT ACAACAACAG 
AGAGAAGAAA AGGATCAGCA TGGGTTACAA CTGCAAAGTG AAATCAATCA ATTACATAGC 
AAACTTCTGG AGACAGAGCG CCAACTAGGA GAAGCTCATG GAAGGCTGAA GGAACAGAGA 
CAGCTTTCAA GTGAAAAGTT GATGGATAAA GAACAACAAG TGGCTGATCT ACAACTCAAA 
CTTTCTCGTT TAGAGGAAGA GTTGAAGGAA AAAGTAACCA ATTCCACAGA ATTGCAGCAT 
CAGTTAGATA AAACAAAGCA ACAGCATCAA GAACAACAAG CTCTTCAACA AAGCACTACA 
GCAAAACTTC GAGAAGCTCA GAATGATTTG GAACAAGTAC TACGTCAAAT TGGTGATAAG 
GACCAAAAGA TCCAGAACCT TGAAGCCTTA TTACAGAAGA GCAAAGAAAA TATTTCTTTA 
CTAGAAAAGG AGAGAGAAGA TCTTTATGCA AAAATTCAGG CTGGTGAGGG AGAGACTGCT 
GTTCTTAACC AATTACAAGA AAAAAACCAT ACATTACAGG AACAAGTAAC TCAGCTAACA 
GAGAAGCTGA AGAATCAGTC AGAAAGCCAT AAACAAGCCC AGGAGAATTT GCATGACCAG 
GTGCAGGAGC AGAAGGCACA TCTTAGAGCT GCACAAGACC GTGTCCTTTC CCTAGAATCC 
AGCATCAATG AATTAAATAG TCAGTTAAAT GAAAGCAAGG AGAAGGTTTC CCAGCTTGAT 
ATACAGGTTA AAGCCAAAAC TGAATTATTG CTATCAGCAG AAGCAGCAAA AACTGCTCAG 
AGAGCAGATC TTCAGAATCA TCTGGACACA GCCCAAAATG CATTACAAGA TAAACAGCAG 
GAGTTAAATA AGATCACTAC TCAGTTGGAT CAGGTCACTA CAAAGTTGCA GGACAAGCAA 
GAACATTGCA GTCAGCTGGA AAGTCATCTT AAAGAATACA AAGAGAAACA TCTGTCTTTA 
GAACAGAAAA CTGAAGAATT AGAAGGTCAA ATCAAGAAAC TAGAAGCTGA TATGCTTGAA 
GTTAAAGCAA GCAAGGAGCA AGCTTTGCAG GGTCTACAAC AACAGAGACA GCTGAACACA 
GATTTAGAGC TTCGAGCCAC AGAATTGAGT AAACAACTTG AAATGGAGAA AGAAACAGTA 
TCTAATACAA AGTTGGATCT ACAGAAGAAA TCTGAAGCAC TTGAAAATAC TAAGCAAATG 
CTTACCAAGC AAGAAGAAGA AAAAACAATG CTTAAACAAG AAATTGAAAA TTTAAGTCAA 
GATGCAAAGA TGCAGCACAA GGAACTGAAT GATAGGATTC AAACAGCAGT AACAGAACTA 
CAGAAAGTGA AGGTGGAGAA AGACTCTCTG GTAGCAGAGC TTTCTGCAGC AAAAGAGAAA 
CTGTCAAAAG TTTCTGATTG TTTGAAGAAC TCCCAAAGTG AATTTGAAAA AGAAAATCAG 
AAGGGAAAAG CTGTTATATT AGATTTGGAG AAAACTTGCA AAGAATTAAA GCATCAGCTT 
CAAGTACAGA CAGAAAGCCT GCATAAGGAG CAGAATGAAA TGAAAAAGTC ACTTGAAAAA 
GAAAAGGAGA CTTCTCATCA GTTGAAGTTG GAGCTCAGTT CCATGCAGGG ACAAGTCATA 
CAGGCCCAGG ACAGCTTAAA ACAAAAGGAA AAAGAAGAAC AACAGCTTCA GAGTAACATA 
AATGAGCTAA AGCAATTGAC TGAACAGAAG AAAAAGCAAA TTGAAACACT CCAAGGAGAA 
GTTAAAATTG CTGTTTCACA GAAGACAGAA CTTGAGAATA AACTACAGCA GCAGTCAATG 
CAAGCAGCAC AGGAACTTGC AGCAGAGAAA CAAAAAATAT CAGTGTTACA AAACACCTAT 
GAAAAAAGTC AGGAAAATCT AAAACAGCTT CAGTCGGATT TCTATGGGAA GGAATCTGAA 
CTACTTGCCA CAAGGCAGGA TCTTAAGTCT ATAGAAGAGA AGCTTTCTCT AGCACAGGAG 
GATTTGATAT CAAACAGAAA TCAAATTGGA AACCAAAATA AATTGATTCA AGAACTGAAG 
ACCACCAAGA CTACTTTGGA GCAAGATTTA GCAAAGAAAG AACAGCAATT GAAGGAACAG 
AATAAAGCAC TACAAGATAT GCAAAAAGAA AAGTCACTAA AAGAAAAAGA ACTAGTAAAT 
GAAAAATCTA AATTGGCAGA GACAGAGGAA ATTAAGTGTA GACAAGAAAA AGAAATTGCT 
AAATTGAGTG AAGAACTCAA GTCTCACAAG CAGGAAAGCA TAAAGGAAAT AACAAATCTC 
AAGGATGCCA AACAGCTTTT GATTCAGCAG AAATTAGAGC TTCAAGGGAA AGTAGATTCC 
CTGAAGGCAA CCCTTGAACA GGAGAAGAAA ACCCAGCAAA TGCTAAAAGA GCAGATGAAA 
AAGGAAGAAG ATGAGCTGAA GAAAGAGTTT ATGGAAAAGG AAGCTAAACT GCATTCCGAA 
ATAAAAGAAA AAGAAGTAGG AATGAAGAAG CATGAAGAAA ATGGGGCTAA ACTTACCATG 
CAGATCACAG CATTAAATGA AAACTTGGGC ACCATAAAGA AAGAGTGGCA GTCCAGTCAA 
CGGAGAGTCA GTGAACTCGA GAAACAGACG GACGACTTAC GGGGTGAAAT TGCAGTACTG 
GAAGCGACAG TTCAGAATAA CCAAGATGAA AGGAGAGCAC TGCTGGAAAG GTGTCTTAAA 
GGAGAAGGTG AAATAGAAAA GCTTCAAACC AAAGTACTAG AATTGCAAAG AAAGCTGGAT 
AATACAACTG CAGCAGTGCA GGAGCTAGGC AGAGAGAATC AGTCCCTTCA AATCAAACAT 
ACACAGGCGT TGACTAGAAA GTGGGCTGAA GACAGTGAAG TACAAAACTG TATGGCCTGT 
GGGAAAGGCT TTTCCGTAAC AGTGAGACGG CATCACTGCA GACAATGTGG AAATATCTTC 
TGTGCTGAGT GTTCAGCCAA AAATGCCTTA ACTCCTTCTT CCAAGAAGCC TGTTCGTGTC 
TGTGATGGAT GTTTCAATGA CTTGCAAGGA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_594411.6
CDS	259..4491
Translation	MLRRILQRTPGRVGSQGSDLDSSAAPINTVDVNNESSSEGFICPQCMKSLGSADELFKHYEAVHDAGNDSSHGGEALALKRDDITLLRQEVQDLQASLKEEKWYSEELKKELEKFQGLQQQESKPDGLVADSSAELQSLEQQLEEAQTENFNIKQMKDLFEQKAAQLATEIADIKSKYDEERSLREAAEQQVTHLTEELNKEAAVIQDLKTELLQRPGIEDVAVLKKELVQVQTLMDNMTLERERESEKLKDECKKLQTEYTNSEAVISQLRSELAKGPQEVAVYVQELQKLKSSVNELTQKNQNLTEKLQKKELDYTQLEEKHNEECMSKKSIQASLHQKDLDCQQLQSRLSASETSLQRIQAELGEKGEATQKLKEELSEVETKYQHLKAEFKQLQQQREEKDQHGLQLQSEINQLHSKLLETERQLGEAHGRLKEQRQLSSEKLMDKEQQVADLQLKLSRLEEELKEKVTNSTELQHQLDKTKQQHQEQQALQQSTTAKLREAQNDLEQVLRQIGDKDQKIQNLEALLQKSKENISLLEKEREDLYAKIQAGEGETAVLNQLQEKNHTLQEQVTQLTEKLKNQSESHKQAQENLHDQVQEQKAHLRAAQDRVLSLESSINELNSQLNESKEKVSQLDIQVKAKTELLLSAEAAKTAQRADLQNHLDTAQNALQDKQQELNKITTQLDQVTTKLQDKQEHCSQLESHLKEYKEKHLSLEQKTEELEGQIKKLEADMLEVKASKEQALQGLQQQRQLNTDLELRATELSKQLEMEKETVSNTKLDLQKKSEALENTKQMLTKQEEEKTMLKQEIENLSQDAKMQHKELNDRIQTAVTELQKVKVEKDSLVAELSAAKEKLSKVSDCLKNSQSEFEKENQKGKAVILDLEKTCKELKHQLQVQTESLHKEQNEMKKSLEKEKETSHQLKLELSSMQGQVIQAQDSLKQKEKEEQQLQSNINELKQLTEQKKKQIETLQGEVKIAVSQKTELENKLQQQSMQAAQELAAEKQKISVLQNTYEKSQENLKQLQSDFYGKESELLATRQDLKSIEEKLSLAQEDLISNRNQIGNQNKLIQELKTTKTTLEQDLAKKEQQLKEQNKALQDMQKEKSLKEKELVNEKSKLAETEEIKCRQEKEIAKLSEELKSHKQESIKEITNLKDAKQLLIQQKLELQGKVDSLKATLEQEKKTQQMLKEQMKKEEDELKKEFMEKEAKLHSEIKEKEVGMKKHEENGAKLTMQITALNENLGTIKKEWQSSQRRVSELEKQTDDLRGEIAVLEATVQNNQDERRALLERCLKGEGEIEKLQTKVLELQRKLDNTTAAVQELGRENQSLQIKHTQALTRKWAEDSEVQNCMACGKGFSVTVRRHHCRQCGNIFCAECSAKNALTPSSKKPVRVCDGCFNDLQG

Target ORF information:

RefSeq Version	XM_594411.9
Organism	Bos taurus(cattle)
Definition	Bos taurus early endosome antigen 1 (EEA1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_594411.9

ORF Insert Sequence:

ATGTTAAGGA GGATTCTACA GAGGACTCCT GGGAGAGTTG GCTCTCAAGG TTCAGATCTA 
GATTCATCAG CAGCTCCTAT AAACACAGTG GATGTCAATA ATGAAAGCTC TTCAGAGGGT 
TTCATATGTC CACAGTGTAT GAAATCTCTT GGATCTGCTG ATGAACTTTT CAAACACTAT 
GAGGCAGTCC ATGATGCTGG TAATGACTCA AGTCACGGAG GAGAAGCTTT GGCTCTGAAG 
CGAGATGATA TAACACTACT CAGACAAGAG GTCCAAGACC TACAGGCTTC ACTTAAGGAA 
GAAAAATGGT ACTCAGAAGA ATTAAAGAAA GAATTAGAAA AATTTCAAGG GCTGCAGCAA 
CAAGAGTCCA AACCTGATGG ATTAGTGGCT GATTCATCAG CAGAACTACA GTCTTTGGAA 
CAACAATTAG AAGAAGCCCA AACAGAAAAT TTTAATATTA AACAAATGAA AGATTTGTTT 
GAACAGAAAG CAGCCCAACT TGCTACTGAA ATTGCAGATA TAAAGTCAAA ATATGATGAA 
GAAAGGAGTC TTAGAGAAGC TGCTGAACAG CAAGTGACAC ATCTAACAGA AGAATTAAAC 
AAAGAAGCAG CTGTGATTCA AGATCTAAAG ACTGAACTGC TTCAGAGACC TGGAATAGAA 
GATGTTGCTG TATTAAAGAA AGAACTGGTC CAAGTTCAAA CACTAATGGA TAACATGACC 
TTGGAACGTG AGAGAGAATC TGAAAAACTC AAAGATGAGT GCAAAAAGTT ACAAACAGAA 
TATACTAACT CAGAGGCCGT AATAAGCCAG CTGAGGAGTG AACTTGCCAA AGGCCCGCAG 
GAAGTTGCTG TGTATGTGCA GGAACTACAA AAACTTAAAA GTTCAGTTAA TGAATTAACA 
CAAAAAAATC AGAACTTGAC TGAAAAGTTA CAGAAGAAAG AACTGGACTA TACTCAGTTA 
GAAGAGAAAC ATAATGAAGA ATGTATGAGT AAAAAGAGTA TACAAGCAAG CCTTCATCAA 
AAAGACCTGG ATTGTCAACA GCTTCAGTCA AGGTTATCTG CATCTGAAAC CTCACTGCAG 
AGAATACAGG CAGAACTAGG TGAAAAGGGA GAAGCTACTC AGAAGCTCAA AGAAGAATTA 
TCAGAAGTGG AGACCAAGTA CCAGCATCTT AAGGCAGAGT TTAAACAGCT ACAACAACAG 
AGAGAAGAAA AGGATCAGCA TGGGTTACAA CTGCAAAGTG AAATCAATCA ATTACATAGC 
AAACTTCTGG AGACAGAGCG CCAACTAGGA GAAGCTCATG GAAGGCTGAA GGAACAGAGA 
CAGCTTTCAA GTGAAAAGTT GATGGATAAA GAACAACAAG TGGCTGATCT ACAACTCAAA 
CTTTCTCGTT TAGAGGAAGA GTTGAAGGAA AAAGTAACCA ATTCCACAGA ATTGCAGCAT 
CAGTTAGATA AAACAAAGCA ACAGCATCAA GAACAACAAG CTCTTCAACA AAGCACTACA 
GCAAAACTTC GAGAAGCTCA GAATGATTTG GAACAAGTAC TACGTCAAAT TGGTGATAAG 
GACCAAAAGA TCCAGAACCT TGAAGCCTTA TTACAGAAGA GCAAAGAAAA TATTTCTTTA 
CTAGAAAAGG AGAGAGAAGA TCTTTATGCA AAAATTCAGG CTGGTGAGGG AGAGACTGCT 
GTTCTTAACC AATTACAAGA AAAAAACCAT ACATTACAGG AACAAGTAAC TCAGCTAACA 
GAGAAGCTGA AGAATCAGTC AGAAAGCCAT AAACAAGCCC AGGAGAATTT GCATGACCAG 
GTGCAGGAGC AGAAGGCACA TCTTAGAGCT GCACAAGACC GTGTCCTTTC CCTAGAATCC 
AGCATCAATG AATTAAATAG TCAGTTAAAT GAAAGCAAGG AGAAGGTTTC CCAGCTTGAT 
ATACAGGTTA AAGCCAAAAC TGAATTATTG CTATCAGCAG AAGCAGCAAA AACTGCTCAG 
AGAGCAGATC TTCAGAATCA TCTGGACACA GCCCAAAATG CATTACAAGA TAAACAGCAG 
GAGTTAAATA AGATCACTAC TCAGTTGGAT CAGGTCACTA CAAAGTTGCA GGACAAGCAA 
GAACATTGCA GTCAGCTGGA AAGTCATCTT AAAGAATACA AAGAGAAACA TCTGTCTTTA 
GAACAGAAAA CTGAAGAATT AGAAGGTCAA ATCAAGAAAC TAGAAGCTGA TATGCTTGAA 
GTTAAAGCAA GCAAGGAGCA AGCTTTGCAG GGTCTACAAC AACAGAGACA GCTGAACACA 
GATTTAGAGC TTCGAGCCAC AGAATTGAGT AAACAACTTG AAATGGAGAA AGAAACAGTA 
TCTAATACAA AGTTGGATCT ACAGAAGAAA TCTGAAGCAC TTGAAAATAC TAAGCAAATG 
CTTACCAAGC AAGAAGAAGA AAAAACAATG CTTAAACAAG AAATTGAAAA TTTAAGTCAA 
GATGCAAAGA TGCAGCACAA GGAACTGAAT GATAGGATTC AAACAGCAGT AACAGAACTA 
CAGAAAGTGA AGGTGGAGAA AGACTCTCTG GTAGCAGAGC TTTCTGCAGC AAAAGAGAAA 
CTGTCAAAAG TTTCTGATTG TTTGAAGAAC TCCCAAAGTG AATTTGAAAA AGAAAATCAG 
AAGGGAAAAG CTGTTATATT AGATTTGGAG AAAACTTGCA AAGAATTAAA GCATCAGCTT 
CAAGTACAGA CAGAAAGCCT GCATAAGGAG CAGAATGAAA TGAAAAAGTC ACTTGAAAAA 
GAAAAGGAGA CTTCTCATCA GTTGAAGTTG GAGCTCAGTT CCATGCAGGG ACAAGTCATA 
CAGGCCCAGG ACAGCTTAAA ACAAAAGGAA AAAGAAGAAC AACAGCTTCA GAGTAACATA 
AATGAGCTAA AGCAATTGAC TGAACAGAAG AAAAAGCAAA TTGAAACACT CCAAGGAGAA 
GTTAAAATTG CTGTTTCACA GAAGACAGAA CTTGAGAATA AACTACAGCA GCAGTCAATG 
CAAGCAGCAC AGGAACTTGC AGCAGAGAAA CAAAAAATAT CAGTGTTACA AAACACCTAT 
GAAAAAAGTC AGGAAAATCT AAAACAGCTT CAGTCGGATT TCTATGGGAA GGAATCTGAA 
CTACTTGCCA CAAGGCAGGA TCTTAAGTCT ATAGAAGAGA AGCTTTCTCT AGCACAGGAG 
GATTTGATAT CAAACAGAAA TCAAATTGGA AACCAAAATA AATTGATTCA AGAACTGAAG 
ACCACCAAGA CTACTTTGGA GCAAGATTTA GCAAAGAAAG AACAGCAATT GAAGGAACAG 
AATAAAGCAC TACAAGATAT GCAAAAAGAA AAGTCACTAA AAGAAAAAGA ACTAGTAAAT 
GAAAAATCTA AATTGGCAGA GACAGAGGAA ATTAAGTGTA GACAAGAAAA AGAAATTGCT 
AAATTGAGTG AAGAACTCAA GTCTCACAAG CAGGAAAGCA TAAAGGAAAT AACAAATCTC 
AAGGATGCCA AACAGCTTTT GATTCAGCAG AAATTAGAGC TTCAAGGGAA AGTAGATTCC 
CTGAAGGCAA CCCTTGAACA GGAGAAGAAA ACCCAGCAAA TGCTAAAAGA GCAGATGAAA 
AAGGAAGAAG ATGAGCTGAA GAAAGAGTTT ATGGAAAAGG AAGCTAAACT GCATTCCGAA 
ATAAAAGAAA AAGAAGTAGG AATGAAGAAG CATGAAGAAA ATGGGGCTAA ACTTACCATG 
CAGATCACAG CATTAAATGA AAACTTGGGC ACCATAAAGA AAGAGTGGCA GTCCAGTCAA 
CGGAGAGTCA GTGAACTCGA GAAACAGACG GACGACTTAC GGGGTGAAAT TGCAGTACTG 
GAAGCGACAG TTCAGAATAA CCAAGATGAA AGGAGAGCAC TGCTGGAAAG GTGTCTTAAA 
GGAGAAGGTG AAATAGAAAA GCTTCAAACC AAAGTACTAG AATTGCAAAG AAAGCTGGAT 
AATACAACTG CAGCAGTGCA GGAGCTAGGC AGAGAGAATC AGTCCCTTCA AATCAAACAT 
ACACAGGCGT TGACTAGAAA GTGGGCTGAA GACAGTGAAG TACAAAACTG TATGGCCTGT 
GGGAAAGGCT TTTCCGTAAC AGTGAGACGG CATCACTGCA GACAATGTGG AAATATCTTC 
TGTGCTGAGT GTTCAGCCAA AAATGCCTTA ACTCCTTCTT CCAAGAAGCC TGTTCGTGTC 
TGTGATGGAT GTTTCAATGA CTTGCAAGGA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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EEA1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

オンラインフォーム

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