eea1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	eea1
Entrez Gene ID	100216061
Full Name	early endosome antigen 1
Synonyms	zfyve2
General protein information	Preferred Names early endosome antigen 1 Names early endosome antigen 1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002935315.4	XP_002935361.1	early endosome antigen 1 isoform X1
XM_012959889.2	XP_012815343.1	early endosome antigen 1 isoform X2
XM_004912917.3	XP_004912974.2	early endosome antigen 1 isoform X3
XM_002935315.5	XP_002935361.2	early endosome antigen 1 isoform X1
XM_018092240.1	XP_017947729.1	early endosome antigen 1 isoform X4
XM_018092239.1	XP_017947728.1	early endosome antigen 1 isoform X2
XM_012959889.3	XP_012815343.2	early endosome antigen 1 isoform X2
XM_004912917.4	XP_004912974.3	early endosome antigen 1 isoform X3

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Eea1	NP_001101556.1
Canis lupus familiaris (dog)	EEA1	XP_005629490.1
Gallus gallus (chicken)	EEA1	XP_004937680.1
Xenopus tropicalis (tropical clawed frog)	eea1	XP_002935361.1
Homo sapiens (human)	EEA1	NP_003557.2
	EEA1	XP_001104577.1
Mus musculus (house mouse)	Eea1	NP_001001932.1
Pan troglodytes (chimpanzee)	EEA1	XP_522610.2
Bos taurus (cattle)	EEA1	XP_005206182.1
Danio rerio (zebrafish)	LOC562943	XP_003200485.1

Gene Ontology

The following eea1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the eea1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa43231	XM_018092240.1 Latest version!	Xenopus tropicalis early endosome antigen 1 (eea1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OXa54681	XM_002935315.5 Latest version!	Xenopus tropicalis early endosome antigen 1 (eea1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa54682	XM_012959889.3 Latest version!	Xenopus tropicalis early endosome antigen 1 (eea1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa54683	XM_004912917.4 Latest version!	Xenopus tropicalis early endosome antigen 1 (eea1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa32539	XM_004912917.3 Latest version!	Xenopus tropicalis early endosome antigen 1 (eea1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa07736	XM_002935315.4 Latest version!	Xenopus tropicalis early endosome antigen 1 (eea1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa32538	XM_012959889.2 Latest version!	Xenopus tropicalis early endosome antigen 1 (eea1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa32538	XM_018092239.1 Latest version!	Xenopus tropicalis early endosome antigen 1 (eea1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

eea1 ( XM_018092240.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017947729.1 Xenopus tropicalis early endosome antigen 1 (eea1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXa43231
Clone ID Related Accession (Same CDS sequence)	XM_018092240.1
Accession Version	XM_018092240.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3606bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	early endosome antigen 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTGTT TTGTTGCCTT GCAGCTTCAG CGACCTGGGA TTGAAGATGT TGCTGTGTTG 
AAGAAGGAGC TTGTCCAGGT CCAGACGCTG ATGGACAAAA CAACGCTAGA TGCTGAAAAT 
GAATCAGAGA AGCTGAAAGA TGAGTGCAGA CAGCTGCAGG CACAAATCTC TAACTCTGAG 
GTGACTATTA CTCAACTGAA AGCTGAACTT GCTAAAGGCC CTCAAGAAGT TGCAGTTTAT 
GTCCAGGAAT TGCAGCAACT CAAAACTGCT TTGACTGAGT TAAATCAAAA ACATGATAGT 
TTGATGGAAA AATTTTTGAA GAAGGAATTG GATTACACCC AGCTTGAGGA GAAACAAAAT 
GAGGAGTCCA CTGCAAGGAA AAACCTGCAA GTCCTTCTCA ATCAGAAGGA CGTGGAGTTC 
CAGCAATTGC AAGCTCGGTT CTCTGCAAGA GACATGTCGC TGCAGACCGT TAAGGCTGAG 
GTGGGCGAGA AGGCTGAGAC AAATCAGAAA CTAAAAGCGG AACTCTCAGA AGTCGAAACC 
AAATATCAGC ATCTCAAAGC CGAATTTAAG CAGCTTCAGC AGCAACGGGA AGAGAAGGAG 
CAGCTTAGTT TGCAGCAGCA AAGTGAGATC AATCAGCTTC ACAGCAAGTT ACTGGAGACA 
GAGCGCCAAC TAGGGGAAGC TCATGGGAGG CTGAAGGAGC AGAGGCAGCT GTCTGCTGAG 
AAACTGATGG ACAAGGAGCA GCAGGTGGCT GATCTACAGC TGAAACTCTC ACGTGCTGAG 
GAGCAGCTCA AAGAAAAGAC TTCCGCGTCT ACTGACTTGC AGCATCAATT GGAAAAGATA 
AGGCAACAGC ATCAGGAACA GACCAACCTC CAGCAGAATA CCACATCCAA ATTGAGAGAA 
GCCCAAAATG ATTTAGAACA AGTCTTACGG CAGATTGGGG ACAAAGACCA GAAAATCCAG 
AACCTTGAAG CACTATTGGA GAAAAGCAAA AAGAACATTT CTCTTCTTGA AAAAGAACGA 
GAAGATTTAT ATGCCAAAAT TCAAGCTGGT GAAGGAGAAA CTGCAGTTCT AAACCAACTT 
CAAGAGAAAA ACCACGCTTT GCAGGAACAG TTAACCCAGC TTACTGAGAA GTTAAAAAAT 
CAATCAGAAA GTCACAAACA AGCCCAGGAA AACTTGCATG AGCAGGTTCA GGAGCAGAAG 
TCCCAACTCA GAGCCTCCCA AGATCGTGTC CTGGCACTTG AGGCAACACT TAATGAAGTC 
ACCAGTCAGC TAAATGAGAG CAAAGAGAAA ATGGTTCAAC TGGATTTACA GATCAAAGCA 
AAAACTGAAC TTTTGTTAGC AGCAGAGGCA GCAAAGACAG CGCAAAGAGT AGATCTTCAG 
AACCACTTGG ATACAGCCCA AAATGCACTG CAGGATAAGC AACAGGAATT AAACAAAGTG 
AACGCACAAC TGGAACAAGT AACAGCAAAG TTAAATGACA AGCAGGAGTA CAGCATTCAA 
CTGGAGGCGA ACCTTAAGGA GTACAAAGAG AAGCACCTTA ATCTGGAACA AAAGTCTGAA 
GCTCTGGAAG GGCAGCTTAA GAAACTTGAA ACAGATCTAA TCAGTGTGAA AGCAAACCGG 
GAACAGACAA CCCAGGAGCT GCAGCAGCAG AAGCAGCAGA GCGCTCAGAT GGAATTGAAA 
TCTGCAGAGC TGAGCAAGCA GTTAGAAGAG GAGAAAGGAC TGGTATCAAG TATGAAACTG 
GAACTACAGA AGAAAGTGGA GTCTCTCCAG CAGTCAAAGG AATTCATTTC AAAACAAGAT 
CAAGAAAGGA ACAGTTTGAA GGAGAATATA GAAAAGTTAA AGCAAGACCT GGAGAAAATA 
TCAGGCAAAA TGCAAGATGC CGTGACAAGC CTAGAGAAAA CCAAACTGGA GAGAGACGCT 
TTGCAAAAAG ATCTTGCAGA GACCAAGAAG AAATTATCTG AGAGCAGCAA ATCTTTACAG 
GAATCAAGAG ATCAACTGGA AAAAGAAAAA CTGAAAGGAA AGAAAACAGT TGAAAACATG 
GAGAAGTCAC AGGAAGAAGT GAAATTGAAT CTACAAAGAC AGGTGGATGC AACTGCAAAG 
GAGGTGAATG AGCTCAAGTC TATGCTGGAA AATAAGCAGG AGGAATCCAC CCAGACAAAG 
GCTGAGTTGG ATAAAGCCCG TGGCTACGTT GCAAAGCTGC ACAGTGCTAT CAAAGAAAAG 
GAGAAAGCTG AGCAGGAGCT CCAGACAGAA CTGAAAAAGA TGCATGAAAC TTCAGATCAG 
GAGAAAGTGC AACACAAATC TGAGATTGAA GCCATGACCA CTCAGTTAGC ACAAAAGGCA 
GAACAAGAAG GAAAATTACA GCAGCAAGTG AAAGGATTAA CCAGTGAACT GGAAACTGAG 
AAAGGAAAAG TGTCTGATCT GCAGAAGTCC CACAAACTCC TTCAGGAGAA AGTGAATAGC 
CTTCAGTCAG ATATGTACGG GAAAGAGTCA GAAGTATCTG CAATACGGCA GGATCTGAAA 
AATACAGAGG AAAAGCTTAT TCTTGCACAG GAAGAGCTAA AAACAAACAG GAATGAAGTC 
AATGGCCACA AAAAGCTTAT ACAAGAACTA ACTGCTGTGA GAGGGACCTT GGAGCAGGAT 
ATAGCTAAAA ATAAGAACTT GCTGAAGGAA CGGGAAAAAA CACTGCAGGA ACTGCAGGAG 
CAGAAGGTCT TAATAGAGAA GGACATAAGT GATGAAAAAC TGAAAGTAAC TAATCTAAAC 
TCCTTAAATG AGAAGCTTAA GGGAGAATTA ATCCAGACTA GAGAAGAACT AAAGAGCATC 
AAAGATGAAT GCCAAAAGAA AACCAGCAGT TTTGAGGATG CCAAACAGCT ACTAATCCAG 
CAGAAGCTGG AGTTGCAGGC CAAACTTGAC AGCTCAGCTT CAGTTCTACA ACAGGAAAAG 
AATAGCCAAC AGGCTCTGAA GGAGGACATC AAGAAAAAGC AAGAGGACTG GAAAAAACAT 
AGCACGGAGA TGAGAGAGAA ATTGTCTGCA GAAGCAAAAG AAAAAGAAGA ACAAAAGAAG 
AAACATGAGG AGAATGAGTC AAAGCTAAAC ATGCAGATTA CAGCTTTAAA TGAGAACCTG 
GCAACGTTAA AGAAGGAATG GCAGAGCAGC CAACGTAGGG TCGGTGAGCT GGAGAAGCAA 
ACAGACGACC TGCGGGGAGA GATTGCAGTA TTAGAAGCAA CAGTCCAAAA CAATCAGGAC 
GACAGACGTG CTTTGTTAGA AAGGTGTCTT AAAAGCGAGG GTGAAATTGA AAAGCTCCAA 
GCCAAAATTT TCGAACTGCG GAAAAAAGTA GATGAATCTT CTTATGCAAT GGAGGAACTT 
GGCAGGGAAA ACCAATCACT ACAGATAAAG CACAACCAAG CACTCAGTAG GAAGTGGACA 
GAGGACCACG AAGTGCAGAA CTGCATGTCA TGTGGCAGAG GATTTTCTGT AACAATAAGA 
AGGCACCACT GTAGACAGTG TGGAAATATC TTCTGTCATG AATGTTCCTC GAAGAATGCC 
TTAACGCCCT CCTCCAAAAA AGCTGTGCGT GTTTGTGATA CATGTTTTGG TGACCTGCAA 
GGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017947729.1
CDS	81..3686
Translation	MSCFVALQLQRPGIEDVAVLKKELVQVQTLMDKTTLDAENESEKLKDECRQLQAQISNSEVTITQLKAELAKGPQEVAVYVQELQQLKTALTELNQKHDSLMEKFLKKELDYTQLEEKQNEESTARKNLQVLLNQKDVEFQQLQARFSARDMSLQTVKAEVGEKAETNQKLKAELSEVETKYQHLKAEFKQLQQQREEKEQLSLQQQSEINQLHSKLLETERQLGEAHGRLKEQRQLSAEKLMDKEQQVADLQLKLSRAEEQLKEKTSASTDLQHQLEKIRQQHQEQTNLQQNTTSKLREAQNDLEQVLRQIGDKDQKIQNLEALLEKSKKNISLLEKEREDLYAKIQAGEGETAVLNQLQEKNHALQEQLTQLTEKLKNQSESHKQAQENLHEQVQEQKSQLRASQDRVLALEATLNEVTSQLNESKEKMVQLDLQIKAKTELLLAAEAAKTAQRVDLQNHLDTAQNALQDKQQELNKVNAQLEQVTAKLNDKQEYSIQLEANLKEYKEKHLNLEQKSEALEGQLKKLETDLISVKANREQTTQELQQQKQQSAQMELKSAELSKQLEEEKGLVSSMKLELQKKVESLQQSKEFISKQDQERNSLKENIEKLKQDLEKISGKMQDAVTSLEKTKLERDALQKDLAETKKKLSESSKSLQESRDQLEKEKLKGKKTVENMEKSQEEVKLNLQRQVDATAKEVNELKSMLENKQEESTQTKAELDKARGYVAKLHSAIKEKEKAEQELQTELKKMHETSDQEKVQHKSEIEAMTTQLAQKAEQEGKLQQQVKGLTSELETEKGKVSDLQKSHKLLQEKVNSLQSDMYGKESEVSAIRQDLKNTEEKLILAQEELKTNRNEVNGHKKLIQELTAVRGTLEQDIAKNKNLLKEREKTLQELQEQKVLIEKDISDEKLKVTNLNSLNEKLKGELIQTREELKSIKDECQKKTSSFEDAKQLLIQQKLELQAKLDSSASVLQQEKNSQQALKEDIKKKQEDWKKHSTEMREKLSAEAKEKEEQKKKHEENESKLNMQITALNENLATLKKEWQSSQRRVGELEKQTDDLRGEIAVLEATVQNNQDDRRALLERCLKSEGEIEKLQAKIFELRKKVDESSYAMEELGRENQSLQIKHNQALSRKWTEDHEVQNCMSCGRGFSVTIRRHHCRQCGNIFCHECSSKNALTPSSKKAVRVCDTCFGDLQG

Target ORF information:

RefSeq Version	XM_018092240.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis early endosome antigen 1 (eea1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018092240.1

ORF Insert Sequence:

ATGAGCTGTT TTGTTGCCTT GCAGCTTCAG CGACCTGGGA TTGAAGATGT TGCTGTGTTG 
AAGAAGGAGC TTGTCCAGGT CCAGACGCTG ATGGACAAAA CAACGCTAGA TGCTGAAAAT 
GAATCAGAGA AGCTGAAAGA TGAGTGCAGA CAGCTGCAGG CACAAATCTC TAACTCTGAG 
GTGACTATTA CTCAACTGAA AGCTGAACTT GCTAAAGGCC CTCAAGAAGT TGCAGTTTAT 
GTCCAGGAAT TGCAGCAACT CAAAACTGCT TTGACTGAGT TAAATCAAAA ACATGATAGT 
TTGATGGAAA AATTTTTGAA GAAGGAATTG GATTACACCC AGCTTGAGGA GAAACAAAAT 
GAGGAGTCCA CTGCAAGGAA AAACCTGCAA GTCCTTCTCA ATCAGAAGGA CGTGGAGTTC 
CAGCAATTGC AAGCTCGGTT CTCTGCAAGA GACATGTCGC TGCAGACCGT TAAGGCTGAG 
GTGGGCGAGA AGGCTGAGAC AAATCAGAAA CTAAAAGCGG AACTCTCAGA AGTCGAAACC 
AAATATCAGC ATCTCAAAGC CGAATTTAAG CAGCTTCAGC AGCAACGGGA AGAGAAGGAG 
CAGCTTAGTT TGCAGCAGCA AAGTGAGATC AATCAGCTTC ACAGCAAGTT ACTGGAGACA 
GAGCGCCAAC TAGGGGAAGC TCATGGGAGG CTGAAGGAGC AGAGGCAGCT GTCTGCTGAG 
AAACTGATGG ACAAGGAGCA GCAGGTGGCT GATCTACAGC TGAAACTCTC ACGTGCTGAG 
GAGCAGCTCA AAGAAAAGAC TTCCGCGTCT ACTGACTTGC AGCATCAATT GGAAAAGATA 
AGGCAACAGC ATCAGGAACA GACCAACCTC CAGCAGAATA CCACATCCAA ATTGAGAGAA 
GCCCAAAATG ATTTAGAACA AGTCTTACGG CAGATTGGGG ACAAAGACCA GAAAATCCAG 
AACCTTGAAG CACTATTGGA GAAAAGCAAA AAGAACATTT CTCTTCTTGA AAAAGAACGA 
GAAGATTTAT ATGCCAAAAT TCAAGCTGGT GAAGGAGAAA CTGCAGTTCT AAACCAACTT 
CAAGAGAAAA ACCACGCTTT GCAGGAACAG TTAACCCAGC TTACTGAGAA GTTAAAAAAT 
CAATCAGAAA GTCACAAACA AGCCCAGGAA AACTTGCATG AGCAGGTTCA GGAGCAGAAG 
TCCCAACTCA GAGCCTCCCA AGATCGTGTC CTGGCACTTG AGGCAACACT TAATGAAGTC 
ACCAGTCAGC TAAATGAGAG CAAAGAGAAA ATGGTTCAAC TGGATTTACA GATCAAAGCA 
AAAACTGAAC TTTTGTTAGC AGCAGAGGCA GCAAAGACAG CGCAAAGAGT AGATCTTCAG 
AACCACTTGG ATACAGCCCA AAATGCACTG CAGGATAAGC AACAGGAATT AAACAAAGTG 
AACGCACAAC TGGAACAAGT AACAGCAAAG TTAAATGACA AGCAGGAGTA CAGCATTCAA 
CTGGAGGCGA ACCTTAAGGA GTACAAAGAG AAGCACCTTA ATCTGGAACA AAAGTCTGAA 
GCTCTGGAAG GGCAGCTTAA GAAACTTGAA ACAGATCTAA TCAGTGTGAA AGCAAACCGG 
GAACAGACAA CCCAGGAGCT GCAGCAGCAG AAGCAGCAGA GCGCTCAGAT GGAATTGAAA 
TCTGCAGAGC TGAGCAAGCA GTTAGAAGAG GAGAAAGGAC TGGTATCAAG TATGAAACTG 
GAACTACAGA AGAAAGTGGA GTCTCTCCAG CAGTCAAAGG AATTCATTTC AAAACAAGAT 
CAAGAAAGGA ACAGTTTGAA GGAGAATATA GAAAAGTTAA AGCAAGACCT GGAGAAAATA 
TCAGGCAAAA TGCAAGATGC CGTGACAAGC CTAGAGAAAA CCAAACTGGA GAGAGACGCT 
TTGCAAAAAG ATCTTGCAGA GACCAAGAAG AAATTATCTG AGAGCAGCAA ATCTTTACAG 
GAATCAAGAG ATCAACTGGA AAAAGAAAAA CTGAAAGGAA AGAAAACAGT TGAAAACATG 
GAGAAGTCAC AGGAAGAAGT GAAATTGAAT CTACAAAGAC AGGTGGATGC AACTGCAAAG 
GAGGTGAATG AGCTCAAGTC TATGCTGGAA AATAAGCAGG AGGAATCCAC CCAGACAAAG 
GCTGAGTTGG ATAAAGCCCG TGGCTACGTT GCAAAGCTGC ACAGTGCTAT CAAAGAAAAG 
GAGAAAGCTG AGCAGGAGCT CCAGACAGAA CTGAAAAAGA TGCATGAAAC TTCAGATCAG 
GAGAAAGTGC AACACAAATC TGAGATTGAA GCCATGACCA CTCAGTTAGC ACAAAAGGCA 
GAACAAGAAG GAAAATTACA GCAGCAAGTG AAAGGATTAA CCAGTGAACT GGAAACTGAG 
AAAGGAAAAG TGTCTGATCT GCAGAAGTCC CACAAACTCC TTCAGGAGAA AGTGAATAGC 
CTTCAGTCAG ATATGTACGG GAAAGAGTCA GAAGTATCTG CAATACGGCA GGATCTGAAA 
AATACAGAGG AAAAGCTTAT TCTTGCACAG GAAGAGCTAA AAACAAACAG GAATGAAGTC 
AATGGCCACA AAAAGCTTAT ACAAGAACTA ACTGCTGTGA GAGGGACCTT GGAGCAGGAT 
ATAGCTAAAA ATAAGAACTT GCTGAAGGAA CGGGAAAAAA CACTGCAGGA ACTGCAGGAG 
CAGAAGGTCT TAATAGAGAA GGACATAAGT GATGAAAAAC TGAAAGTAAC TAATCTAAAC 
TCCTTAAATG AGAAGCTTAA GGGAGAATTA ATCCAGACTA GAGAAGAACT AAAGAGCATC 
AAAGATGAAT GCCAAAAGAA AACCAGCAGT TTTGAGGATG CCAAACAGCT ACTAATCCAG 
CAGAAGCTGG AGTTGCAGGC CAAACTTGAC AGCTCAGCTT CAGTTCTACA ACAGGAAAAG 
AATAGCCAAC AGGCTCTGAA GGAGGACATC AAGAAAAAGC AAGAGGACTG GAAAAAACAT 
AGCACGGAGA TGAGAGAGAA ATTGTCTGCA GAAGCAAAAG AAAAAGAAGA ACAAAAGAAG 
AAACATGAGG AGAATGAGTC AAAGCTAAAC ATGCAGATTA CAGCTTTAAA TGAGAACCTG 
GCAACGTTAA AGAAGGAATG GCAGAGCAGC CAACGTAGGG TCGGTGAGCT GGAGAAGCAA 
ACAGACGACC TGCGGGGAGA GATTGCAGTA TTAGAAGCAA CAGTCCAAAA CAATCAGGAC 
GACAGACGTG CTTTGTTAGA AAGGTGTCTT AAAAGCGAGG GTGAAATTGA AAAGCTCCAA 
GCCAAAATTT TCGAACTGCG GAAAAAAGTA GATGAATCTT CTTATGCAAT GGAGGAACTT 
GGCAGGGAAA ACCAATCACT ACAGATAAAG CACAACCAAG CACTCAGTAG GAAGTGGACA 
GAGGACCACG AAGTGCAGAA CTGCATGTCA TGTGGCAGAG GATTTTCTGT AACAATAAGA 
AGGCACCACT GTAGACAGTG TGGAAATATC TTCTGTCATG AATGTTCCTC GAAGAATGCC 
TTAACGCCCT CCTCCAAAAA AGCTGTGCGT GTTTGTGATA CATGTTTTGG TGACCTGCAA 
GGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eea1 ( XM_002935315.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002935361.2 Xenopus tropicalis early endosome antigen 1 (eea1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa54681
Clone ID Related Accession (Same CDS sequence)	XM_002935315.5
Accession Version	XM_002935315.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4209bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	early endosome antigen 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002935315.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGGA GGATTTTACA GCTGACTCCT GGCAGAGTTG GCTCACAGAA CTCTGAATCT 
GACTCATCAG CCACTCCAGG AAATGCTGAA AGCTCATCAG AGGGTTTCAT TTGTCCGCTG 
TGTATGAAAT CCCATGCAAC AGCTGAAGAA CTTTTTGTGC ACTATGAAGC ATCTCACGAA 
CCTGCCAATG ACCCTGGTCA AGGAGGAGAA ACCAATCTGT CGCCAAAAAG AGATGAAGTG 
CTCTTACTTC GTCAGGAAGT CAAAGATTTA CAGACCTCAT TAAAGGAAGA AAAATGGTTT 
TCAGAAGAGT TGAAGAAAGA GTTGGAAAAT GTTCAAGGGC AACTGAAGCA AAACACAATT 
TCAGATGGGA TGACCACAAT TGCAGCTACA GAGCTTCAGT CTTTAGAACA GAAGTTAGAA 
GAGGCCCAGA CAGAAATGTT TAATATAAAG CAAATGAAGG ATTTATTTGA ACAGAAAGCA 
GCTCAACAGG CTACTGAAGT AGTTGATTTA AAGGCATCAT ATGATGAAGA GAAGAGTAAC 
TTGGCAGCTG CACGGGAGAA AGTAAAAGGT TTAACACAAG ATCTGGATTC AAAGTTACAC 
GTGATTGAAG ACTTGAAGAA TGAGCTGCTT CAGCGACCTG GGATTGAAGA TGTTGCTGTG 
TTGAAGAAGG AGCTTGTCCA GGTCCAGACG CTGATGGACA AAACAACGCT AGATGCTGAA 
AAAGAATCAG AGAAGCTGAA AGATGAGTGC AGACAGCTGC AGGCACAAAT CTCTAACTCT 
GAGGTGACTA TTACTCAACT GAAAGCTGAA CTTGCTAAAG GCCCTCAAGA AGTTGCAGTT 
TATGTCCAGG AATTGCAGCA ACTCAAAACT GCTTTGACTG AGTTAAATCA AAAACATGAT 
AGTTTGATGG AAAAATTTTT GAAGAAGGAA TTGGATTACA CCCAGCTTGA GGAGAAACAA 
AATGAGGAGT CCACTGCAAG GAAAAACCTG CAAGTCCTTC TCAATCAGAA GGACGTGGAG 
TTCCAGCAAT TGCAAGCTCG GCTCTCTGCA AGAGACATGT CGCTGCAGAC CGTTAAGGCT 
GAGGTGGGCG AGAAGGCTGA GACAAATCAG AAACTAAAAG CGGAACTCTC AGAAGTCGAA 
ACCAAATATC AGCATCTCAA AGCCGAATTT AAGCAGCTTC AGCAGCAACG GGAAGAGAAG 
GAGCAGCTTA GTTTGCAGCA GCAAAGTGAG ATCAATCAGC TTCACAGCAA GTTACTGGAG 
ACAGAGCGCC AACTAGGGGA AGCTCATGGG AGGCTGAAGG AGCAGAGGCA GCTGTCTGCT 
GAGAAACTGA TGGACAAGGA GCAGCAGGTG GCTGATCTAC AGCTGAAACT CTCACGTGCT 
GAGGAGCAGC TCAAAGAAAA GACTTCCGCG TCTACTGACT TGCAGCATCA ATTGGAAAAG 
ATAAGGCAAC AGCATCAGGA ACAGACCAAC CTCCAGCAGA ATACCACATC CAAATTGAGA 
GAAGCCCAAA ATGATTTAGA ACAAGTCTTA CGGCAGATTG GGGACAAAGA CCAGAAAATC 
CAGAACCTTG AAGCACTATT GGAGAAAAGC AAAAAGAACA TTTCTCTTCT TGAAAAAGAA 
CGAGAAGATT TATATGCCAA AATTCAAGCT GGTGAAGGAG AAACTGCAGT TCTAAACCAA 
CTTCAAGAGA AAAACCACGC TTTGCAGGAA CAGTTAACCC AGCTTACAGA GAAGTTAAAA 
AATCAATCAG AAAGTCACAA ACAAGCCCAG GAAAACTTGC ATGAGCAGGT TCAGGAGCAG 
AAGTCCCAAC TCAGAGCCTC CCAAGATCGT GTCCTGGCAC TTGAGGCAAC ACTTAATGAA 
GTCACCAGTC AGCTAAATGA GAGCAAAGAG AAAATGGTTC AACTGGATTT ACAGATCAAA 
GCAAAAACTG AACTTTTGTT AGCAGCAGAG GCAGCAAAGA CAGCGCAAAG AGTAGATCTT 
CAGAACCACT TGGATACAGC CCAAAATGCA CTGCAGGATA AGCAACAGGA ATTAAACAAA 
GTGAACGCAC AACTGGAACA AGTAACAGCA AAGTTAAATG ACAAGCAGGA GTACAGCATT 
CAACTGGAGG CGAACCTTAA GGAGTACAAA GAGAAGCACC TTAATCTGGA ACAGAAGTCT 
GAAGCTCTGG AAGGGCAGCT TAAGAAACTT GAAACAGATC TAATCAGTGT GAAAGCAAAC 
CGGGAACAGA CAACCCAGGA GCTGCAGCAG CAGAAGCAGC AGAGCGCTCA GATGGAATTG 
AAATCTGCAG AGCTGAGCAA GCAGTTAGAA GAGGAGAAAG GACTGGTATC AAGCATGAAA 
CTGGAACTAC AGAAGAAAGT GGAGTCTCTC CAGCAGTCAA AGGAATTCAT TTCAAAACAA 
GATCAAGAAA GGAACAGTTT GAAGGAGAAT ATAGAAAAGT TAAAGCAAGA CCTGGAGAAA 
ATATCAGGCA AAATGCAAGA TGCCGTGACA AGCCTAGAGA AAACCAAACT GGAGAGAGAC 
GCTTTGCAAA AAGATCTTGC AGAGACCAAG AAGAAATTAT CTGAGAGCAG CAAATCTTTA 
CAGGAATCAA GAGATCAACT GGAAAAAGAA AAACTGAAAG GAAAGAAAAC AGTTGAAAAC 
ATGGAGAAGT CACAGGAAGA AGTGAAATTG AATCTACAAA GACAGGTGGA TGCAACTGCA 
AAGGAGGTGA ATGAGCTCAA GTCTATGCTG GAAAATAAGC AGGAGGAATC CACCCAGACA 
AAGGCTGAGT TGGATAAAGC CCGTGGCTAC GTTGCAAAGC TGCACAGTGC TATCAAAGAA 
AAGGAGAAAG CTGAGCAGGA GCTCCAGACA GAACTGAAAA AGATGCATGA AACTTCAGAT 
CAGGAGAAAG TGCAACACAA ATCTGAGATT GAAGCCATGA CCACTCAGTT AGCACAAAAG 
GCAGAACAAG AAGGAAAATT ACAGCAGCAA GTGAAAGGAT TAACCAGTGA ACTGGAAACT 
GAGAAAGGAA AAGTGTCTGA TCTGCAGAAG TCCCACAAAC TCCTTCAGGA GAAAGTGAAT 
AGCCTTCAGT CAGATATGTA CGGGAAAGAG TCAGAAGTAT CTGCAATACG GCAGGATCTG 
AAAAATACAG AGGAAAAGCT TATTCTTGCA CAGGAAGAGC TAAAAACAAA CAGGAATGAA 
GTCAATGGCC ACAAAAAGCT TATACAAGAA CTAACTGCTG TGAGAGGGAC CTTGGAGCAG 
GATATAGCTA AAAATAAGAA CTTGCTGAAG GAACGGGAAA AAATACTGCA GGAACTGCAG 
GAGCAGAAGG TCTTAATAGA GAAGGACATA AGTGATGAAA AACTGAAAGT AACTAATCTA 
AACTCCTTAA ATGAGAAGCT TAAGGGAGAA TTAATCCAGA CTAGAGAAGA ACTAAAGAGC 
ATCAAAGATG AATGCCAAAA GAAAACCAGC AGTTTTGAGG ATGCCAAACA GCTACTAATC 
CAGCAGAAGC TGGAGTTGCA GGCCAAACTT GACAGCTCAG CTTCAGTTCT ACAACAGGAA 
AAGAATAGCC AACAGGCTCT GAAGGAGGAC ATCAAGAAAA AGCAAGAGGA CTGGAAAAAA 
CATAGCACGG AGATGAGAGA GAAATTGTCT GCAGAAGCAA AAGAAAAAGA AGAACAAAAG 
AAGAAACATG AGGAGAATGA GTCAAAGCTA AACATGCAGA TTACAGCTTT AAATGAGAAC 
CTGGCAACGT TAAAGAAGGA ATGGCAGAGC AGCCAACGTA GGGTCGGTGA GCTGGAGAAG 
CAAACAGACG ACCTGCGGGG AGAGATTGCA GTATTAGAAG CAACAGTCCA AAACAATCAG 
GACGACAGAC GTGCTTTGTT AGAAAGGTGT CTTAAAAGTG AGGGTGAAAT TGAAAAGCTC 
CAAGCCAAAA TTTTCGAACT GCGGAAAAAA GTAGATGAAT CTTCTTATGC AATGGAGGAA 
CTTGGCAGGG AAAACCAATC ACTACAGATA AAGCACAACC AAGCACTCAG TAGGAAGTGG 
ACAGAGGACC ACGAAGTGCA GAACTGCATG TCATGTGGCA GAGGATTTTC TGTAACAATA 
AGAAGGCACC ACTGTAGACA GTGTGGAAAT ATCTTCTGTC ATGAATGTTC CTCGAAGAAT 
GCCTTAACGC CCTCCTCCAA AAAAGCTGTG CGTGTTTGTG ATACATGTTT TGGTGACCTG 
CAAGGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002935361.2
CDS	255..4463
Translation	MFRRILQLTPGRVGSQNSESDSSATPGNAESSSEGFICPLCMKSHATAEELFVHYEASHEPANDPGQGGETNLSPKRDEVLLLRQEVKDLQTSLKEEKWFSEELKKELENVQGQLKQNTISDGMTTIAATELQSLEQKLEEAQTEMFNIKQMKDLFEQKAAQQATEVVDLKASYDEEKSNLAAAREKVKGLTQDLDSKLHVIEDLKNELLQRPGIEDVAVLKKELVQVQTLMDKTTLDAEKESEKLKDECRQLQAQISNSEVTITQLKAELAKGPQEVAVYVQELQQLKTALTELNQKHDSLMEKFLKKELDYTQLEEKQNEESTARKNLQVLLNQKDVEFQQLQARLSARDMSLQTVKAEVGEKAETNQKLKAELSEVETKYQHLKAEFKQLQQQREEKEQLSLQQQSEINQLHSKLLETERQLGEAHGRLKEQRQLSAEKLMDKEQQVADLQLKLSRAEEQLKEKTSASTDLQHQLEKIRQQHQEQTNLQQNTTSKLREAQNDLEQVLRQIGDKDQKIQNLEALLEKSKKNISLLEKEREDLYAKIQAGEGETAVLNQLQEKNHALQEQLTQLTEKLKNQSESHKQAQENLHEQVQEQKSQLRASQDRVLALEATLNEVTSQLNESKEKMVQLDLQIKAKTELLLAAEAAKTAQRVDLQNHLDTAQNALQDKQQELNKVNAQLEQVTAKLNDKQEYSIQLEANLKEYKEKHLNLEQKSEALEGQLKKLETDLISVKANREQTTQELQQQKQQSAQMELKSAELSKQLEEEKGLVSSMKLELQKKVESLQQSKEFISKQDQERNSLKENIEKLKQDLEKISGKMQDAVTSLEKTKLERDALQKDLAETKKKLSESSKSLQESRDQLEKEKLKGKKTVENMEKSQEEVKLNLQRQVDATAKEVNELKSMLENKQEESTQTKAELDKARGYVAKLHSAIKEKEKAEQELQTELKKMHETSDQEKVQHKSEIEAMTTQLAQKAEQEGKLQQQVKGLTSELETEKGKVSDLQKSHKLLQEKVNSLQSDMYGKESEVSAIRQDLKNTEEKLILAQEELKTNRNEVNGHKKLIQELTAVRGTLEQDIAKNKNLLKEREKILQELQEQKVLIEKDISDEKLKVTNLNSLNEKLKGELIQTREELKSIKDECQKKTSSFEDAKQLLIQQKLELQAKLDSSASVLQQEKNSQQALKEDIKKKQEDWKKHSTEMREKLSAEAKEKEEQKKKHEENESKLNMQITALNENLATLKKEWQSSQRRVGELEKQTDDLRGEIAVLEATVQNNQDDRRALLERCLKSEGEIEKLQAKIFELRKKVDESSYAMEELGRENQSLQIKHNQALSRKWTEDHEVQNCMSCGRGFSVTIRRHHCRQCGNIFCHECSSKNALTPSSKKAVRVCDTCFGDLQG

Target ORF information:

RefSeq Version	XM_002935315.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis early endosome antigen 1 (eea1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002935315.5

ORF Insert Sequence:

ATGTTCAGGA GGATTTTACA GCTGACTCCT GGCAGAGTTG GCTCACAGAA CTCTGAATCT 
GACTCATCAG CCACTCCAGG AAATGCTGAA AGCTCATCAG AGGGTTTCAT TTGTCCGCTG 
TGTATGAAAT CCCATGCAAC AGCTGAAGAA CTTTTTGTGC ACTATGAAGC ATCTCACGAA 
CCTGCCAATG ACCCTGGTCA AGGAGGAGAA ACCAATCTGT CGCCAAAAAG AGATGAAGTG 
CTCTTACTTC GTCAGGAAGT CAAAGATTTA CAGACCTCAT TAAAGGAAGA AAAATGGTTT 
TCAGAAGAGT TGAAGAAAGA GTTGGAAAAT GTTCAAGGGC AACTGAAGCA AAACACAATT 
TCAGATGGGA TGACCACAAT TGCAGCTACA GAGCTTCAGT CTTTAGAACA GAAGTTAGAA 
GAGGCCCAGA CAGAAATGTT TAATATAAAG CAAATGAAGG ATTTATTTGA ACAGAAAGCA 
GCTCAACAGG CTACTGAAGT AGTTGATTTA AAGGCATCAT ATGATGAAGA GAAGAGTAAC 
TTGGCAGCTG CACGGGAGAA AGTAAAAGGT TTAACACAAG ATCTGGATTC AAAGTTACAC 
GTGATTGAAG ACTTGAAGAA TGAGCTGCTT CAGCGACCTG GGATTGAAGA TGTTGCTGTG 
TTGAAGAAGG AGCTTGTCCA GGTCCAGACG CTGATGGACA AAACAACGCT AGATGCTGAA 
AAAGAATCAG AGAAGCTGAA AGATGAGTGC AGACAGCTGC AGGCACAAAT CTCTAACTCT 
GAGGTGACTA TTACTCAACT GAAAGCTGAA CTTGCTAAAG GCCCTCAAGA AGTTGCAGTT 
TATGTCCAGG AATTGCAGCA ACTCAAAACT GCTTTGACTG AGTTAAATCA AAAACATGAT 
AGTTTGATGG AAAAATTTTT GAAGAAGGAA TTGGATTACA CCCAGCTTGA GGAGAAACAA 
AATGAGGAGT CCACTGCAAG GAAAAACCTG CAAGTCCTTC TCAATCAGAA GGACGTGGAG 
TTCCAGCAAT TGCAAGCTCG GCTCTCTGCA AGAGACATGT CGCTGCAGAC CGTTAAGGCT 
GAGGTGGGCG AGAAGGCTGA GACAAATCAG AAACTAAAAG CGGAACTCTC AGAAGTCGAA 
ACCAAATATC AGCATCTCAA AGCCGAATTT AAGCAGCTTC AGCAGCAACG GGAAGAGAAG 
GAGCAGCTTA GTTTGCAGCA GCAAAGTGAG ATCAATCAGC TTCACAGCAA GTTACTGGAG 
ACAGAGCGCC AACTAGGGGA AGCTCATGGG AGGCTGAAGG AGCAGAGGCA GCTGTCTGCT 
GAGAAACTGA TGGACAAGGA GCAGCAGGTG GCTGATCTAC AGCTGAAACT CTCACGTGCT 
GAGGAGCAGC TCAAAGAAAA GACTTCCGCG TCTACTGACT TGCAGCATCA ATTGGAAAAG 
ATAAGGCAAC AGCATCAGGA ACAGACCAAC CTCCAGCAGA ATACCACATC CAAATTGAGA 
GAAGCCCAAA ATGATTTAGA ACAAGTCTTA CGGCAGATTG GGGACAAAGA CCAGAAAATC 
CAGAACCTTG AAGCACTATT GGAGAAAAGC AAAAAGAACA TTTCTCTTCT TGAAAAAGAA 
CGAGAAGATT TATATGCCAA AATTCAAGCT GGTGAAGGAG AAACTGCAGT TCTAAACCAA 
CTTCAAGAGA AAAACCACGC TTTGCAGGAA CAGTTAACCC AGCTTACAGA GAAGTTAAAA 
AATCAATCAG AAAGTCACAA ACAAGCCCAG GAAAACTTGC ATGAGCAGGT TCAGGAGCAG 
AAGTCCCAAC TCAGAGCCTC CCAAGATCGT GTCCTGGCAC TTGAGGCAAC ACTTAATGAA 
GTCACCAGTC AGCTAAATGA GAGCAAAGAG AAAATGGTTC AACTGGATTT ACAGATCAAA 
GCAAAAACTG AACTTTTGTT AGCAGCAGAG GCAGCAAAGA CAGCGCAAAG AGTAGATCTT 
CAGAACCACT TGGATACAGC CCAAAATGCA CTGCAGGATA AGCAACAGGA ATTAAACAAA 
GTGAACGCAC AACTGGAACA AGTAACAGCA AAGTTAAATG ACAAGCAGGA GTACAGCATT 
CAACTGGAGG CGAACCTTAA GGAGTACAAA GAGAAGCACC TTAATCTGGA ACAGAAGTCT 
GAAGCTCTGG AAGGGCAGCT TAAGAAACTT GAAACAGATC TAATCAGTGT GAAAGCAAAC 
CGGGAACAGA CAACCCAGGA GCTGCAGCAG CAGAAGCAGC AGAGCGCTCA GATGGAATTG 
AAATCTGCAG AGCTGAGCAA GCAGTTAGAA GAGGAGAAAG GACTGGTATC AAGCATGAAA 
CTGGAACTAC AGAAGAAAGT GGAGTCTCTC CAGCAGTCAA AGGAATTCAT TTCAAAACAA 
GATCAAGAAA GGAACAGTTT GAAGGAGAAT ATAGAAAAGT TAAAGCAAGA CCTGGAGAAA 
ATATCAGGCA AAATGCAAGA TGCCGTGACA AGCCTAGAGA AAACCAAACT GGAGAGAGAC 
GCTTTGCAAA AAGATCTTGC AGAGACCAAG AAGAAATTAT CTGAGAGCAG CAAATCTTTA 
CAGGAATCAA GAGATCAACT GGAAAAAGAA AAACTGAAAG GAAAGAAAAC AGTTGAAAAC 
ATGGAGAAGT CACAGGAAGA AGTGAAATTG AATCTACAAA GACAGGTGGA TGCAACTGCA 
AAGGAGGTGA ATGAGCTCAA GTCTATGCTG GAAAATAAGC AGGAGGAATC CACCCAGACA 
AAGGCTGAGT TGGATAAAGC CCGTGGCTAC GTTGCAAAGC TGCACAGTGC TATCAAAGAA 
AAGGAGAAAG CTGAGCAGGA GCTCCAGACA GAACTGAAAA AGATGCATGA AACTTCAGAT 
CAGGAGAAAG TGCAACACAA ATCTGAGATT GAAGCCATGA CCACTCAGTT AGCACAAAAG 
GCAGAACAAG AAGGAAAATT ACAGCAGCAA GTGAAAGGAT TAACCAGTGA ACTGGAAACT 
GAGAAAGGAA AAGTGTCTGA TCTGCAGAAG TCCCACAAAC TCCTTCAGGA GAAAGTGAAT 
AGCCTTCAGT CAGATATGTA CGGGAAAGAG TCAGAAGTAT CTGCAATACG GCAGGATCTG 
AAAAATACAG AGGAAAAGCT TATTCTTGCA CAGGAAGAGC TAAAAACAAA CAGGAATGAA 
GTCAATGGCC ACAAAAAGCT TATACAAGAA CTAACTGCTG TGAGAGGGAC CTTGGAGCAG 
GATATAGCTA AAAATAAGAA CTTGCTGAAG GAACGGGAAA AAATACTGCA GGAACTGCAG 
GAGCAGAAGG TCTTAATAGA GAAGGACATA AGTGATGAAA AACTGAAAGT AACTAATCTA 
AACTCCTTAA ATGAGAAGCT TAAGGGAGAA TTAATCCAGA CTAGAGAAGA ACTAAAGAGC 
ATCAAAGATG AATGCCAAAA GAAAACCAGC AGTTTTGAGG ATGCCAAACA GCTACTAATC 
CAGCAGAAGC TGGAGTTGCA GGCCAAACTT GACAGCTCAG CTTCAGTTCT ACAACAGGAA 
AAGAATAGCC AACAGGCTCT GAAGGAGGAC ATCAAGAAAA AGCAAGAGGA CTGGAAAAAA 
CATAGCACGG AGATGAGAGA GAAATTGTCT GCAGAAGCAA AAGAAAAAGA AGAACAAAAG 
AAGAAACATG AGGAGAATGA GTCAAAGCTA AACATGCAGA TTACAGCTTT AAATGAGAAC 
CTGGCAACGT TAAAGAAGGA ATGGCAGAGC AGCCAACGTA GGGTCGGTGA GCTGGAGAAG 
CAAACAGACG ACCTGCGGGG AGAGATTGCA GTATTAGAAG CAACAGTCCA AAACAATCAG 
GACGACAGAC GTGCTTTGTT AGAAAGGTGT CTTAAAAGTG AGGGTGAAAT TGAAAAGCTC 
CAAGCCAAAA TTTTCGAACT GCGGAAAAAA GTAGATGAAT CTTCTTATGC AATGGAGGAA 
CTTGGCAGGG AAAACCAATC ACTACAGATA AAGCACAACC AAGCACTCAG TAGGAAGTGG 
ACAGAGGACC ACGAAGTGCA GAACTGCATG TCATGTGGCA GAGGATTTTC TGTAACAATA 
AGAAGGCACC ACTGTAGACA GTGTGGAAAT ATCTTCTGTC ATGAATGTTC CTCGAAGAAT 
GCCTTAACGC CCTCCTCCAA AAAAGCTGTG CGTGTTTGTG ATACATGTTT TGGTGACCTG 
CAAGGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eea1 ( XM_012959889.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012815343.2 Xenopus tropicalis early endosome antigen 1 (eea1), transcript variant X2, mRNA.

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CloneID	OXa54682
Clone ID Related Accession (Same CDS sequence)	XM_012959889.3
Accession Version	XM_012959889.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4086bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	early endosome antigen 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959889.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCC ATGCAACAGC TGAAGAACTT TTTGTGCACT ATGAAGCATC TCACGAACCT 
GCCAATGACC CTGGTCAAGG AGGAGAAACC AATCTGTCGC CAAAAAGAGA TGAAGTGCTC 
TTACTTCGTC AGGAAGTCAA AGATTTACAG ACCTCATTAA AGGAAGAAAA ATGGTTTTCA 
GAAGAGTTGA AGAAAGAGTT GGAAAATGTT CAAGGGCAAC TGAAGCAAAA CACAATTTCA 
GATGGGATGA CCACAATTGC AGCTACAGAG CTTCAGTCTT TAGAACAGAA GTTAGAAGAG 
GCCCAGACAG AAATGTTTAA TATAAAGCAA ATGAAGGATT TATTTGAACA GAAAGCAGCT 
CAACAGGCTA CTGAAGTAGT TGATTTAAAG GCATCATATG ATGAAGAGAA GAGTAACTTG 
GCAGCTGCAC GGGAGAAAGT AAAAGGTTTA ACACAAGATC TGGATTCAAA GTTACACGTG 
ATTGAAGACT TGAAGAATGA GCTGCTTCAG CGACCTGGGA TTGAAGATGT TGCTGTGTTG 
AAGAAGGAGC TTGTCCAGGT CCAGACGCTG ATGGACAAAA CAACGCTAGA TGCTGAAAAA 
GAATCAGAGA AGCTGAAAGA TGAGTGCAGA CAGCTGCAGG CACAAATCTC TAACTCTGAG 
GTGACTATTA CTCAACTGAA AGCTGAACTT GCTAAAGGCC CTCAAGAAGT TGCAGTTTAT 
GTCCAGGAAT TGCAGCAACT CAAAACTGCT TTGACTGAGT TAAATCAAAA ACATGATAGT 
TTGATGGAAA AATTTTTGAA GAAGGAATTG GATTACACCC AGCTTGAGGA GAAACAAAAT 
GAGGAGTCCA CTGCAAGGAA AAACCTGCAA GTCCTTCTCA ATCAGAAGGA CGTGGAGTTC 
CAGCAATTGC AAGCTCGGCT CTCTGCAAGA GACATGTCGC TGCAGACCGT TAAGGCTGAG 
GTGGGCGAGA AGGCTGAGAC AAATCAGAAA CTAAAAGCGG AACTCTCAGA AGTCGAAACC 
AAATATCAGC ATCTCAAAGC CGAATTTAAG CAGCTTCAGC AGCAACGGGA AGAGAAGGAG 
CAGCTTAGTT TGCAGCAGCA AAGTGAGATC AATCAGCTTC ACAGCAAGTT ACTGGAGACA 
GAGCGCCAAC TAGGGGAAGC TCATGGGAGG CTGAAGGAGC AGAGGCAGCT GTCTGCTGAG 
AAACTGATGG ACAAGGAGCA GCAGGTGGCT GATCTACAGC TGAAACTCTC ACGTGCTGAG 
GAGCAGCTCA AAGAAAAGAC TTCCGCGTCT ACTGACTTGC AGCATCAATT GGAAAAGATA 
AGGCAACAGC ATCAGGAACA GACCAACCTC CAGCAGAATA CCACATCCAA ATTGAGAGAA 
GCCCAAAATG ATTTAGAACA AGTCTTACGG CAGATTGGGG ACAAAGACCA GAAAATCCAG 
AACCTTGAAG CACTATTGGA GAAAAGCAAA AAGAACATTT CTCTTCTTGA AAAAGAACGA 
GAAGATTTAT ATGCCAAAAT TCAAGCTGGT GAAGGAGAAA CTGCAGTTCT AAACCAACTT 
CAAGAGAAAA ACCACGCTTT GCAGGAACAG TTAACCCAGC TTACAGAGAA GTTAAAAAAT 
CAATCAGAAA GTCACAAACA AGCCCAGGAA AACTTGCATG AGCAGGTTCA GGAGCAGAAG 
TCCCAACTCA GAGCCTCCCA AGATCGTGTC CTGGCACTTG AGGCAACACT TAATGAAGTC 
ACCAGTCAGC TAAATGAGAG CAAAGAGAAA ATGGTTCAAC TGGATTTACA GATCAAAGCA 
AAAACTGAAC TTTTGTTAGC AGCAGAGGCA GCAAAGACAG CGCAAAGAGT AGATCTTCAG 
AACCACTTGG ATACAGCCCA AAATGCACTG CAGGATAAGC AACAGGAATT AAACAAAGTG 
AACGCACAAC TGGAACAAGT AACAGCAAAG TTAAATGACA AGCAGGAGTA CAGCATTCAA 
CTGGAGGCGA ACCTTAAGGA GTACAAAGAG AAGCACCTTA ATCTGGAACA GAAGTCTGAA 
GCTCTGGAAG GGCAGCTTAA GAAACTTGAA ACAGATCTAA TCAGTGTGAA AGCAAACCGG 
GAACAGACAA CCCAGGAGCT GCAGCAGCAG AAGCAGCAGA GCGCTCAGAT GGAATTGAAA 
TCTGCAGAGC TGAGCAAGCA GTTAGAAGAG GAGAAAGGAC TGGTATCAAG CATGAAACTG 
GAACTACAGA AGAAAGTGGA GTCTCTCCAG CAGTCAAAGG AATTCATTTC AAAACAAGAT 
CAAGAAAGGA ACAGTTTGAA GGAGAATATA GAAAAGTTAA AGCAAGACCT GGAGAAAATA 
TCAGGCAAAA TGCAAGATGC CGTGACAAGC CTAGAGAAAA CCAAACTGGA GAGAGACGCT 
TTGCAAAAAG ATCTTGCAGA GACCAAGAAG AAATTATCTG AGAGCAGCAA ATCTTTACAG 
GAATCAAGAG ATCAACTGGA AAAAGAAAAA CTGAAAGGAA AGAAAACAGT TGAAAACATG 
GAGAAGTCAC AGGAAGAAGT GAAATTGAAT CTACAAAGAC AGGTGGATGC AACTGCAAAG 
GAGGTGAATG AGCTCAAGTC TATGCTGGAA AATAAGCAGG AGGAATCCAC CCAGACAAAG 
GCTGAGTTGG ATAAAGCCCG TGGCTACGTT GCAAAGCTGC ACAGTGCTAT CAAAGAAAAG 
GAGAAAGCTG AGCAGGAGCT CCAGACAGAA CTGAAAAAGA TGCATGAAAC TTCAGATCAG 
GAGAAAGTGC AACACAAATC TGAGATTGAA GCCATGACCA CTCAGTTAGC ACAAAAGGCA 
GAACAAGAAG GAAAATTACA GCAGCAAGTG AAAGGATTAA CCAGTGAACT GGAAACTGAG 
AAAGGAAAAG TGTCTGATCT GCAGAAGTCC CACAAACTCC TTCAGGAGAA AGTGAATAGC 
CTTCAGTCAG ATATGTACGG GAAAGAGTCA GAAGTATCTG CAATACGGCA GGATCTGAAA 
AATACAGAGG AAAAGCTTAT TCTTGCACAG GAAGAGCTAA AAACAAACAG GAATGAAGTC 
AATGGCCACA AAAAGCTTAT ACAAGAACTA ACTGCTGTGA GAGGGACCTT GGAGCAGGAT 
ATAGCTAAAA ATAAGAACTT GCTGAAGGAA CGGGAAAAAA TACTGCAGGA ACTGCAGGAG 
CAGAAGGTCT TAATAGAGAA GGACATAAGT GATGAAAAAC TGAAAGTAAC TAATCTAAAC 
TCCTTAAATG AGAAGCTTAA GGGAGAATTA ATCCAGACTA GAGAAGAACT AAAGAGCATC 
AAAGATGAAT GCCAAAAGAA AACCAGCAGT TTTGAGGATG CCAAACAGCT ACTAATCCAG 
CAGAAGCTGG AGTTGCAGGC CAAACTTGAC AGCTCAGCTT CAGTTCTACA ACAGGAAAAG 
AATAGCCAAC AGGCTCTGAA GGAGGACATC AAGAAAAAGC AAGAGGACTG GAAAAAACAT 
AGCACGGAGA TGAGAGAGAA ATTGTCTGCA GAAGCAAAAG AAAAAGAAGA ACAAAAGAAG 
AAACATGAGG AGAATGAGTC AAAGCTAAAC ATGCAGATTA CAGCTTTAAA TGAGAACCTG 
GCAACGTTAA AGAAGGAATG GCAGAGCAGC CAACGTAGGG TCGGTGAGCT GGAGAAGCAA 
ACAGACGACC TGCGGGGAGA GATTGCAGTA TTAGAAGCAA CAGTCCAAAA CAATCAGGAC 
GACAGACGTG CTTTGTTAGA AAGGTGTCTT AAAAGTGAGG GTGAAATTGA AAAGCTCCAA 
GCCAAAATTT TCGAACTGCG GAAAAAAGTA GATGAATCTT CTTATGCAAT GGAGGAACTT 
GGCAGGGAAA ACCAATCACT ACAGATAAAG CACAACCAAG CACTCAGTAG GAAGTGGACA 
GAGGACCACG AAGTGCAGAA CTGCATGTCA TGTGGCAGAG GATTTTCTGT AACAATAAGA 
AGGCACCACT GTAGACAGTG TGGAAATATC TTCTGTCATG AATGTTCCTC GAAGAATGCC 
TTAACGCCCT CCTCCAAAAA AGCTGTGCGT GTTTGTGATA CATGTTTTGG TGACCTGCAA 
GGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012815343.2
CDS	1094..5179
Translation	MKSHATAEELFVHYEASHEPANDPGQGGETNLSPKRDEVLLLRQEVKDLQTSLKEEKWFSEELKKELENVQGQLKQNTISDGMTTIAATELQSLEQKLEEAQTEMFNIKQMKDLFEQKAAQQATEVVDLKASYDEEKSNLAAAREKVKGLTQDLDSKLHVIEDLKNELLQRPGIEDVAVLKKELVQVQTLMDKTTLDAEKESEKLKDECRQLQAQISNSEVTITQLKAELAKGPQEVAVYVQELQQLKTALTELNQKHDSLMEKFLKKELDYTQLEEKQNEESTARKNLQVLLNQKDVEFQQLQARLSARDMSLQTVKAEVGEKAETNQKLKAELSEVETKYQHLKAEFKQLQQQREEKEQLSLQQQSEINQLHSKLLETERQLGEAHGRLKEQRQLSAEKLMDKEQQVADLQLKLSRAEEQLKEKTSASTDLQHQLEKIRQQHQEQTNLQQNTTSKLREAQNDLEQVLRQIGDKDQKIQNLEALLEKSKKNISLLEKEREDLYAKIQAGEGETAVLNQLQEKNHALQEQLTQLTEKLKNQSESHKQAQENLHEQVQEQKSQLRASQDRVLALEATLNEVTSQLNESKEKMVQLDLQIKAKTELLLAAEAAKTAQRVDLQNHLDTAQNALQDKQQELNKVNAQLEQVTAKLNDKQEYSIQLEANLKEYKEKHLNLEQKSEALEGQLKKLETDLISVKANREQTTQELQQQKQQSAQMELKSAELSKQLEEEKGLVSSMKLELQKKVESLQQSKEFISKQDQERNSLKENIEKLKQDLEKISGKMQDAVTSLEKTKLERDALQKDLAETKKKLSESSKSLQESRDQLEKEKLKGKKTVENMEKSQEEVKLNLQRQVDATAKEVNELKSMLENKQEESTQTKAELDKARGYVAKLHSAIKEKEKAEQELQTELKKMHETSDQEKVQHKSEIEAMTTQLAQKAEQEGKLQQQVKGLTSELETEKGKVSDLQKSHKLLQEKVNSLQSDMYGKESEVSAIRQDLKNTEEKLILAQEELKTNRNEVNGHKKLIQELTAVRGTLEQDIAKNKNLLKEREKILQELQEQKVLIEKDISDEKLKVTNLNSLNEKLKGELIQTREELKSIKDECQKKTSSFEDAKQLLIQQKLELQAKLDSSASVLQQEKNSQQALKEDIKKKQEDWKKHSTEMREKLSAEAKEKEEQKKKHEENESKLNMQITALNENLATLKKEWQSSQRRVGELEKQTDDLRGEIAVLEATVQNNQDDRRALLERCLKSEGEIEKLQAKIFELRKKVDESSYAMEELGRENQSLQIKHNQALSRKWTEDHEVQNCMSCGRGFSVTIRRHHCRQCGNIFCHECSSKNALTPSSKKAVRVCDTCFGDLQG

Target ORF information:

RefSeq Version	XM_012959889.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis early endosome antigen 1 (eea1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012959889.3

ORF Insert Sequence:

ATGAAATCCC ATGCAACAGC TGAAGAACTT TTTGTGCACT ATGAAGCATC TCACGAACCT 
GCCAATGACC CTGGTCAAGG AGGAGAAACC AATCTGTCGC CAAAAAGAGA TGAAGTGCTC 
TTACTTCGTC AGGAAGTCAA AGATTTACAG ACCTCATTAA AGGAAGAAAA ATGGTTTTCA 
GAAGAGTTGA AGAAAGAGTT GGAAAATGTT CAAGGGCAAC TGAAGCAAAA CACAATTTCA 
GATGGGATGA CCACAATTGC AGCTACAGAG CTTCAGTCTT TAGAACAGAA GTTAGAAGAG 
GCCCAGACAG AAATGTTTAA TATAAAGCAA ATGAAGGATT TATTTGAACA GAAAGCAGCT 
CAACAGGCTA CTGAAGTAGT TGATTTAAAG GCATCATATG ATGAAGAGAA GAGTAACTTG 
GCAGCTGCAC GGGAGAAAGT AAAAGGTTTA ACACAAGATC TGGATTCAAA GTTACACGTG 
ATTGAAGACT TGAAGAATGA GCTGCTTCAG CGACCTGGGA TTGAAGATGT TGCTGTGTTG 
AAGAAGGAGC TTGTCCAGGT CCAGACGCTG ATGGACAAAA CAACGCTAGA TGCTGAAAAA 
GAATCAGAGA AGCTGAAAGA TGAGTGCAGA CAGCTGCAGG CACAAATCTC TAACTCTGAG 
GTGACTATTA CTCAACTGAA AGCTGAACTT GCTAAAGGCC CTCAAGAAGT TGCAGTTTAT 
GTCCAGGAAT TGCAGCAACT CAAAACTGCT TTGACTGAGT TAAATCAAAA ACATGATAGT 
TTGATGGAAA AATTTTTGAA GAAGGAATTG GATTACACCC AGCTTGAGGA GAAACAAAAT 
GAGGAGTCCA CTGCAAGGAA AAACCTGCAA GTCCTTCTCA ATCAGAAGGA CGTGGAGTTC 
CAGCAATTGC AAGCTCGGCT CTCTGCAAGA GACATGTCGC TGCAGACCGT TAAGGCTGAG 
GTGGGCGAGA AGGCTGAGAC AAATCAGAAA CTAAAAGCGG AACTCTCAGA AGTCGAAACC 
AAATATCAGC ATCTCAAAGC CGAATTTAAG CAGCTTCAGC AGCAACGGGA AGAGAAGGAG 
CAGCTTAGTT TGCAGCAGCA AAGTGAGATC AATCAGCTTC ACAGCAAGTT ACTGGAGACA 
GAGCGCCAAC TAGGGGAAGC TCATGGGAGG CTGAAGGAGC AGAGGCAGCT GTCTGCTGAG 
AAACTGATGG ACAAGGAGCA GCAGGTGGCT GATCTACAGC TGAAACTCTC ACGTGCTGAG 
GAGCAGCTCA AAGAAAAGAC TTCCGCGTCT ACTGACTTGC AGCATCAATT GGAAAAGATA 
AGGCAACAGC ATCAGGAACA GACCAACCTC CAGCAGAATA CCACATCCAA ATTGAGAGAA 
GCCCAAAATG ATTTAGAACA AGTCTTACGG CAGATTGGGG ACAAAGACCA GAAAATCCAG 
AACCTTGAAG CACTATTGGA GAAAAGCAAA AAGAACATTT CTCTTCTTGA AAAAGAACGA 
GAAGATTTAT ATGCCAAAAT TCAAGCTGGT GAAGGAGAAA CTGCAGTTCT AAACCAACTT 
CAAGAGAAAA ACCACGCTTT GCAGGAACAG TTAACCCAGC TTACAGAGAA GTTAAAAAAT 
CAATCAGAAA GTCACAAACA AGCCCAGGAA AACTTGCATG AGCAGGTTCA GGAGCAGAAG 
TCCCAACTCA GAGCCTCCCA AGATCGTGTC CTGGCACTTG AGGCAACACT TAATGAAGTC 
ACCAGTCAGC TAAATGAGAG CAAAGAGAAA ATGGTTCAAC TGGATTTACA GATCAAAGCA 
AAAACTGAAC TTTTGTTAGC AGCAGAGGCA GCAAAGACAG CGCAAAGAGT AGATCTTCAG 
AACCACTTGG ATACAGCCCA AAATGCACTG CAGGATAAGC AACAGGAATT AAACAAAGTG 
AACGCACAAC TGGAACAAGT AACAGCAAAG TTAAATGACA AGCAGGAGTA CAGCATTCAA 
CTGGAGGCGA ACCTTAAGGA GTACAAAGAG AAGCACCTTA ATCTGGAACA GAAGTCTGAA 
GCTCTGGAAG GGCAGCTTAA GAAACTTGAA ACAGATCTAA TCAGTGTGAA AGCAAACCGG 
GAACAGACAA CCCAGGAGCT GCAGCAGCAG AAGCAGCAGA GCGCTCAGAT GGAATTGAAA 
TCTGCAGAGC TGAGCAAGCA GTTAGAAGAG GAGAAAGGAC TGGTATCAAG CATGAAACTG 
GAACTACAGA AGAAAGTGGA GTCTCTCCAG CAGTCAAAGG AATTCATTTC AAAACAAGAT 
CAAGAAAGGA ACAGTTTGAA GGAGAATATA GAAAAGTTAA AGCAAGACCT GGAGAAAATA 
TCAGGCAAAA TGCAAGATGC CGTGACAAGC CTAGAGAAAA CCAAACTGGA GAGAGACGCT 
TTGCAAAAAG ATCTTGCAGA GACCAAGAAG AAATTATCTG AGAGCAGCAA ATCTTTACAG 
GAATCAAGAG ATCAACTGGA AAAAGAAAAA CTGAAAGGAA AGAAAACAGT TGAAAACATG 
GAGAAGTCAC AGGAAGAAGT GAAATTGAAT CTACAAAGAC AGGTGGATGC AACTGCAAAG 
GAGGTGAATG AGCTCAAGTC TATGCTGGAA AATAAGCAGG AGGAATCCAC CCAGACAAAG 
GCTGAGTTGG ATAAAGCCCG TGGCTACGTT GCAAAGCTGC ACAGTGCTAT CAAAGAAAAG 
GAGAAAGCTG AGCAGGAGCT CCAGACAGAA CTGAAAAAGA TGCATGAAAC TTCAGATCAG 
GAGAAAGTGC AACACAAATC TGAGATTGAA GCCATGACCA CTCAGTTAGC ACAAAAGGCA 
GAACAAGAAG GAAAATTACA GCAGCAAGTG AAAGGATTAA CCAGTGAACT GGAAACTGAG 
AAAGGAAAAG TGTCTGATCT GCAGAAGTCC CACAAACTCC TTCAGGAGAA AGTGAATAGC 
CTTCAGTCAG ATATGTACGG GAAAGAGTCA GAAGTATCTG CAATACGGCA GGATCTGAAA 
AATACAGAGG AAAAGCTTAT TCTTGCACAG GAAGAGCTAA AAACAAACAG GAATGAAGTC 
AATGGCCACA AAAAGCTTAT ACAAGAACTA ACTGCTGTGA GAGGGACCTT GGAGCAGGAT 
ATAGCTAAAA ATAAGAACTT GCTGAAGGAA CGGGAAAAAA TACTGCAGGA ACTGCAGGAG 
CAGAAGGTCT TAATAGAGAA GGACATAAGT GATGAAAAAC TGAAAGTAAC TAATCTAAAC 
TCCTTAAATG AGAAGCTTAA GGGAGAATTA ATCCAGACTA GAGAAGAACT AAAGAGCATC 
AAAGATGAAT GCCAAAAGAA AACCAGCAGT TTTGAGGATG CCAAACAGCT ACTAATCCAG 
CAGAAGCTGG AGTTGCAGGC CAAACTTGAC AGCTCAGCTT CAGTTCTACA ACAGGAAAAG 
AATAGCCAAC AGGCTCTGAA GGAGGACATC AAGAAAAAGC AAGAGGACTG GAAAAAACAT 
AGCACGGAGA TGAGAGAGAA ATTGTCTGCA GAAGCAAAAG AAAAAGAAGA ACAAAAGAAG 
AAACATGAGG AGAATGAGTC AAAGCTAAAC ATGCAGATTA CAGCTTTAAA TGAGAACCTG 
GCAACGTTAA AGAAGGAATG GCAGAGCAGC CAACGTAGGG TCGGTGAGCT GGAGAAGCAA 
ACAGACGACC TGCGGGGAGA GATTGCAGTA TTAGAAGCAA CAGTCCAAAA CAATCAGGAC 
GACAGACGTG CTTTGTTAGA AAGGTGTCTT AAAAGTGAGG GTGAAATTGA AAAGCTCCAA 
GCCAAAATTT TCGAACTGCG GAAAAAAGTA GATGAATCTT CTTATGCAAT GGAGGAACTT 
GGCAGGGAAA ACCAATCACT ACAGATAAAG CACAACCAAG CACTCAGTAG GAAGTGGACA 
GAGGACCACG AAGTGCAGAA CTGCATGTCA TGTGGCAGAG GATTTTCTGT AACAATAAGA 
AGGCACCACT GTAGACAGTG TGGAAATATC TTCTGTCATG AATGTTCCTC GAAGAATGCC 
TTAACGCCCT CCTCCAAAAA AGCTGTGCGT GTTTGTGATA CATGTTTTGG TGACCTGCAA 
GGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eea1 ( XM_004912917.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912974.3 Xenopus tropicalis early endosome antigen 1 (eea1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa54683
Clone ID Related Accession (Same CDS sequence)	XM_004912917.4
Accession Version	XM_004912917.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3912bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	early endosome antigen 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004912917.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTTTCA GAAGAGTTGA AGAAAGAGTT GGAAAATGTT CAAGGGCAAC TGAAAACACA 
ATTTCAGATG GGATGACCAC AATTGCAGCT ACAGAGCTTC AGTCTTTAGA ACAGAAGTTA 
GAAGAGGCCC AGACAGAAAT GTTTAATATA AAGCAAATGA AGGATTTATT TGAACAGAAA 
GCAGCTCAAC AGGCTACTGA AGTAGTTGAT TTAAAGGCAT CATATGATGA AGAGAAGAGT 
AACTTGGCAG CTGCACGGGA GAAAGTAAAA GGTTTAACAC AAGATCTGGA TTCAAAGTTA 
CACGTGATTG AAGACTTGAA GAATGAGCTG CTTCAGCGAC CTGGGATTGA AGATGTTGCT 
GTGTTGAAGA AGGAGCTTGT CCAGGTCCAG ACGCTGATGG ACAAAACAAC GCTAGATGCT 
GAAAAAGAAT CAGAGAAGCT GAAAGATGAG TGCAGACAGC TGCAGGCACA AATCTCTAAC 
TCTGAGGTGA CTATTACTCA ACTGAAAGCT GAACTTGCTA AAGGCCCTCA AGAAGTTGCA 
GTTTATGTCC AGGAATTGCA GCAACTCAAA ACTGCTTTGA CTGAGTTAAA TCAAAAACAT 
GATAGTTTGA TGGAAAAATT TTTGAAGAAG GAATTGGATT ACACCCAGCT TGAGGAGAAA 
CAAAATGAGG AGTCCACTGC AAGGAAAAAC CTGCAAGTCC TTCTCAATCA GAAGGACGTG 
GAGTTCCAGC AATTGCAAGC TCGGCTCTCT GCAAGAGACA TGTCGCTGCA GACCGTTAAG 
GCTGAGGTGG GCGAGAAGGC TGAGACAAAT CAGAAACTAA AAGCGGAACT CTCAGAAGTC 
GAAACCAAAT ATCAGCATCT CAAAGCCGAA TTTAAGCAGC TTCAGCAGCA ACGGGAAGAG 
AAGGAGCAGC TTAGTTTGCA GCAGCAAAGT GAGATCAATC AGCTTCACAG CAAGTTACTG 
GAGACAGAGC GCCAACTAGG GGAAGCTCAT GGGAGGCTGA AGGAGCAGAG GCAGCTGTCT 
GCTGAGAAAC TGATGGACAA GGAGCAGCAG GTGGCTGATC TACAGCTGAA ACTCTCACGT 
GCTGAGGAGC AGCTCAAAGA AAAGACTTCC GCGTCTACTG ACTTGCAGCA TCAATTGGAA 
AAGATAAGGC AACAGCATCA GGAACAGACC AACCTCCAGC AGAATACCAC ATCCAAATTG 
AGAGAAGCCC AAAATGATTT AGAACAAGTC TTACGGCAGA TTGGGGACAA AGACCAGAAA 
ATCCAGAACC TTGAAGCACT ATTGGAGAAA AGCAAAAAGA ACATTTCTCT TCTTGAAAAA 
GAACGAGAAG ATTTATATGC CAAAATTCAA GCTGGTGAAG GAGAAACTGC AGTTCTAAAC 
CAACTTCAAG AGAAAAACCA CGCTTTGCAG GAACAGTTAA CCCAGCTTAC AGAGAAGTTA 
AAAAATCAAT CAGAAAGTCA CAAACAAGCC CAGGAAAACT TGCATGAGCA GGTTCAGGAG 
CAGAAGTCCC AACTCAGAGC CTCCCAAGAT CGTGTCCTGG CACTTGAGGC AACACTTAAT 
GAAGTCACCA GTCAGCTAAA TGAGAGCAAA GAGAAAATGG TTCAACTGGA TTTACAGATC 
AAAGCAAAAA CTGAACTTTT GTTAGCAGCA GAGGCAGCAA AGACAGCGCA AAGAGTAGAT 
CTTCAGAACC ACTTGGATAC AGCCCAAAAT GCACTGCAGG ATAAGCAACA GGAATTAAAC 
AAAGTGAACG CACAACTGGA ACAAGTAACA GCAAAGTTAA ATGACAAGCA GGAGTACAGC 
ATTCAACTGG AGGCGAACCT TAAGGAGTAC AAAGAGAAGC ACCTTAATCT GGAACAGAAG 
TCTGAAGCTC TGGAAGGGCA GCTTAAGAAA CTTGAAACAG ATCTAATCAG TGTGAAAGCA 
AACCGGGAAC AGACAACCCA GGAGCTGCAG CAGCAGAAGC AGCAGAGCGC TCAGATGGAA 
TTGAAATCTG CAGAGCTGAG CAAGCAGTTA GAAGAGGAGA AAGGACTGGT ATCAAGCATG 
AAACTGGAAC TACAGAAGAA AGTGGAGTCT CTCCAGCAGT CAAAGGAATT CATTTCAAAA 
CAAGATCAAG AAAGGAACAG TTTGAAGGAG AATATAGAAA AGTTAAAGCA AGACCTGGAG 
AAAATATCAG GCAAAATGCA AGATGCCGTG ACAAGCCTAG AGAAAACCAA ACTGGAGAGA 
GACGCTTTGC AAAAAGATCT TGCAGAGACC AAGAAGAAAT TATCTGAGAG CAGCAAATCT 
TTACAGGAAT CAAGAGATCA ACTGGAAAAA GAAAAACTGA AAGGAAAGAA AACAGTTGAA 
AACATGGAGA AGTCACAGGA AGAAGTGAAA TTGAATCTAC AAAGACAGGT GGATGCAACT 
GCAAAGGAGG TGAATGAGCT CAAGTCTATG CTGGAAAATA AGCAGGAGGA ATCCACCCAG 
ACAAAGGCTG AGTTGGATAA AGCCCGTGGC TACGTTGCAA AGCTGCACAG TGCTATCAAA 
GAAAAGGAGA AAGCTGAGCA GGAGCTCCAG ACAGAACTGA AAAAGATGCA TGAAACTTCA 
GATCAGGAGA AAGTGCAACA CAAATCTGAG ATTGAAGCCA TGACCACTCA GTTAGCACAA 
AAGGCAGAAC AAGAAGGAAA ATTACAGCAG CAAGTGAAAG GATTAACCAG TGAACTGGAA 
ACTGAGAAAG GAAAAGTGTC TGATCTGCAG AAGTCCCACA AACTCCTTCA GGAGAAAGTG 
AATAGCCTTC AGTCAGATAT GTACGGGAAA GAGTCAGAAG TATCTGCAAT ACGGCAGGAT 
CTGAAAAATA CAGAGGAAAA GCTTATTCTT GCACAGGAAG AGCTAAAAAC AAACAGGAAT 
GAAGTCAATG GCCACAAAAA GCTTATACAA GAACTAACTG CTGTGAGAGG GACCTTGGAG 
CAGGATATAG CTAAAAATAA GAACTTGCTG AAGGAACGGG AAAAAATACT GCAGGAACTG 
CAGGAGCAGA AGGTCTTAAT AGAGAAGGAC ATAAGTGATG AAAAACTGAA AGTAACTAAT 
CTAAACTCCT TAAATGAGAA GCTTAAGGGA GAATTAATCC AGACTAGAGA AGAACTAAAG 
AGCATCAAAG ATGAATGCCA AAAGAAAACC AGCAGTTTTG AGGATGCCAA ACAGCTACTA 
ATCCAGCAGA AGCTGGAGTT GCAGGCCAAA CTTGACAGCT CAGCTTCAGT TCTACAACAG 
GAAAAGAATA GCCAACAGGC TCTGAAGGAG GACATCAAGA AAAAGCAAGA GGACTGGAAA 
AAACATAGCA CGGAGATGAG AGAGAAATTG TCTGCAGAAG CAAAAGAAAA AGAAGAACAA 
AAGAAGAAAC ATGAGGAGAA TGAGTCAAAG CTAAACATGC AGATTACAGC TTTAAATGAG 
AACCTGGCAA CGTTAAAGAA GGAATGGCAG AGCAGCCAAC GTAGGGTCGG TGAGCTGGAG 
AAGCAAACAG ACGACCTGCG GGGAGAGATT GCAGTATTAG AAGCAACAGT CCAAAACAAT 
CAGGACGACA GACGTGCTTT GTTAGAAAGG TGTCTTAAAA GTGAGGGTGA AATTGAAAAG 
CTCCAAGCCA AAATTTTCGA ACTGCGGAAA AAAGTAGATG AATCTTCTTA TGCAATGGAG 
GAACTTGGCA GGGAAAACCA ATCACTACAG ATAAAGCACA ACCAAGCACT CAGTAGGAAG 
TGGACAGAGG ACCACGAAGT GCAGAACTGC ATGTCATGTG GCAGAGGATT TTCTGTAACA 
ATAAGAAGGC ACCACTGTAG ACAGTGTGGA AATATCTTCT GTCATGAATG TTCCTCGAAG 
AATGCCTTAA CGCCCTCCTC CAAAAAAGCT GTGCGTGTTT GTGATACATG TTTTGGTGAC 
CTGCAAGGAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912974.3
CDS	98..4009
Translation	MVFRRVEERVGKCSRATENTISDGMTTIAATELQSLEQKLEEAQTEMFNIKQMKDLFEQKAAQQATEVVDLKASYDEEKSNLAAAREKVKGLTQDLDSKLHVIEDLKNELLQRPGIEDVAVLKKELVQVQTLMDKTTLDAEKESEKLKDECRQLQAQISNSEVTITQLKAELAKGPQEVAVYVQELQQLKTALTELNQKHDSLMEKFLKKELDYTQLEEKQNEESTARKNLQVLLNQKDVEFQQLQARLSARDMSLQTVKAEVGEKAETNQKLKAELSEVETKYQHLKAEFKQLQQQREEKEQLSLQQQSEINQLHSKLLETERQLGEAHGRLKEQRQLSAEKLMDKEQQVADLQLKLSRAEEQLKEKTSASTDLQHQLEKIRQQHQEQTNLQQNTTSKLREAQNDLEQVLRQIGDKDQKIQNLEALLEKSKKNISLLEKEREDLYAKIQAGEGETAVLNQLQEKNHALQEQLTQLTEKLKNQSESHKQAQENLHEQVQEQKSQLRASQDRVLALEATLNEVTSQLNESKEKMVQLDLQIKAKTELLLAAEAAKTAQRVDLQNHLDTAQNALQDKQQELNKVNAQLEQVTAKLNDKQEYSIQLEANLKEYKEKHLNLEQKSEALEGQLKKLETDLISVKANREQTTQELQQQKQQSAQMELKSAELSKQLEEEKGLVSSMKLELQKKVESLQQSKEFISKQDQERNSLKENIEKLKQDLEKISGKMQDAVTSLEKTKLERDALQKDLAETKKKLSESSKSLQESRDQLEKEKLKGKKTVENMEKSQEEVKLNLQRQVDATAKEVNELKSMLENKQEESTQTKAELDKARGYVAKLHSAIKEKEKAEQELQTELKKMHETSDQEKVQHKSEIEAMTTQLAQKAEQEGKLQQQVKGLTSELETEKGKVSDLQKSHKLLQEKVNSLQSDMYGKESEVSAIRQDLKNTEEKLILAQEELKTNRNEVNGHKKLIQELTAVRGTLEQDIAKNKNLLKEREKILQELQEQKVLIEKDISDEKLKVTNLNSLNEKLKGELIQTREELKSIKDECQKKTSSFEDAKQLLIQQKLELQAKLDSSASVLQQEKNSQQALKEDIKKKQEDWKKHSTEMREKLSAEAKEKEEQKKKHEENESKLNMQITALNENLATLKKEWQSSQRRVGELEKQTDDLRGEIAVLEATVQNNQDDRRALLERCLKSEGEIEKLQAKIFELRKKVDESSYAMEELGRENQSLQIKHNQALSRKWTEDHEVQNCMSCGRGFSVTIRRHHCRQCGNIFCHECSSKNALTPSSKKAVRVCDTCFGDLQG

Target ORF information:

RefSeq Version	XM_004912917.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis early endosome antigen 1 (eea1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004912917.4

ORF Insert Sequence:

ATGGTTTTCA GAAGAGTTGA AGAAAGAGTT GGAAAATGTT CAAGGGCAAC TGAAAACACA 
ATTTCAGATG GGATGACCAC AATTGCAGCT ACAGAGCTTC AGTCTTTAGA ACAGAAGTTA 
GAAGAGGCCC AGACAGAAAT GTTTAATATA AAGCAAATGA AGGATTTATT TGAACAGAAA 
GCAGCTCAAC AGGCTACTGA AGTAGTTGAT TTAAAGGCAT CATATGATGA AGAGAAGAGT 
AACTTGGCAG CTGCACGGGA GAAAGTAAAA GGTTTAACAC AAGATCTGGA TTCAAAGTTA 
CACGTGATTG AAGACTTGAA GAATGAGCTG CTTCAGCGAC CTGGGATTGA AGATGTTGCT 
GTGTTGAAGA AGGAGCTTGT CCAGGTCCAG ACGCTGATGG ACAAAACAAC GCTAGATGCT 
GAAAAAGAAT CAGAGAAGCT GAAAGATGAG TGCAGACAGC TGCAGGCACA AATCTCTAAC 
TCTGAGGTGA CTATTACTCA ACTGAAAGCT GAACTTGCTA AAGGCCCTCA AGAAGTTGCA 
GTTTATGTCC AGGAATTGCA GCAACTCAAA ACTGCTTTGA CTGAGTTAAA TCAAAAACAT 
GATAGTTTGA TGGAAAAATT TTTGAAGAAG GAATTGGATT ACACCCAGCT TGAGGAGAAA 
CAAAATGAGG AGTCCACTGC AAGGAAAAAC CTGCAAGTCC TTCTCAATCA GAAGGACGTG 
GAGTTCCAGC AATTGCAAGC TCGGCTCTCT GCAAGAGACA TGTCGCTGCA GACCGTTAAG 
GCTGAGGTGG GCGAGAAGGC TGAGACAAAT CAGAAACTAA AAGCGGAACT CTCAGAAGTC 
GAAACCAAAT ATCAGCATCT CAAAGCCGAA TTTAAGCAGC TTCAGCAGCA ACGGGAAGAG 
AAGGAGCAGC TTAGTTTGCA GCAGCAAAGT GAGATCAATC AGCTTCACAG CAAGTTACTG 
GAGACAGAGC GCCAACTAGG GGAAGCTCAT GGGAGGCTGA AGGAGCAGAG GCAGCTGTCT 
GCTGAGAAAC TGATGGACAA GGAGCAGCAG GTGGCTGATC TACAGCTGAA ACTCTCACGT 
GCTGAGGAGC AGCTCAAAGA AAAGACTTCC GCGTCTACTG ACTTGCAGCA TCAATTGGAA 
AAGATAAGGC AACAGCATCA GGAACAGACC AACCTCCAGC AGAATACCAC ATCCAAATTG 
AGAGAAGCCC AAAATGATTT AGAACAAGTC TTACGGCAGA TTGGGGACAA AGACCAGAAA 
ATCCAGAACC TTGAAGCACT ATTGGAGAAA AGCAAAAAGA ACATTTCTCT TCTTGAAAAA 
GAACGAGAAG ATTTATATGC CAAAATTCAA GCTGGTGAAG GAGAAACTGC AGTTCTAAAC 
CAACTTCAAG AGAAAAACCA CGCTTTGCAG GAACAGTTAA CCCAGCTTAC AGAGAAGTTA 
AAAAATCAAT CAGAAAGTCA CAAACAAGCC CAGGAAAACT TGCATGAGCA GGTTCAGGAG 
CAGAAGTCCC AACTCAGAGC CTCCCAAGAT CGTGTCCTGG CACTTGAGGC AACACTTAAT 
GAAGTCACCA GTCAGCTAAA TGAGAGCAAA GAGAAAATGG TTCAACTGGA TTTACAGATC 
AAAGCAAAAA CTGAACTTTT GTTAGCAGCA GAGGCAGCAA AGACAGCGCA AAGAGTAGAT 
CTTCAGAACC ACTTGGATAC AGCCCAAAAT GCACTGCAGG ATAAGCAACA GGAATTAAAC 
AAAGTGAACG CACAACTGGA ACAAGTAACA GCAAAGTTAA ATGACAAGCA GGAGTACAGC 
ATTCAACTGG AGGCGAACCT TAAGGAGTAC AAAGAGAAGC ACCTTAATCT GGAACAGAAG 
TCTGAAGCTC TGGAAGGGCA GCTTAAGAAA CTTGAAACAG ATCTAATCAG TGTGAAAGCA 
AACCGGGAAC AGACAACCCA GGAGCTGCAG CAGCAGAAGC AGCAGAGCGC TCAGATGGAA 
TTGAAATCTG CAGAGCTGAG CAAGCAGTTA GAAGAGGAGA AAGGACTGGT ATCAAGCATG 
AAACTGGAAC TACAGAAGAA AGTGGAGTCT CTCCAGCAGT CAAAGGAATT CATTTCAAAA 
CAAGATCAAG AAAGGAACAG TTTGAAGGAG AATATAGAAA AGTTAAAGCA AGACCTGGAG 
AAAATATCAG GCAAAATGCA AGATGCCGTG ACAAGCCTAG AGAAAACCAA ACTGGAGAGA 
GACGCTTTGC AAAAAGATCT TGCAGAGACC AAGAAGAAAT TATCTGAGAG CAGCAAATCT 
TTACAGGAAT CAAGAGATCA ACTGGAAAAA GAAAAACTGA AAGGAAAGAA AACAGTTGAA 
AACATGGAGA AGTCACAGGA AGAAGTGAAA TTGAATCTAC AAAGACAGGT GGATGCAACT 
GCAAAGGAGG TGAATGAGCT CAAGTCTATG CTGGAAAATA AGCAGGAGGA ATCCACCCAG 
ACAAAGGCTG AGTTGGATAA AGCCCGTGGC TACGTTGCAA AGCTGCACAG TGCTATCAAA 
GAAAAGGAGA AAGCTGAGCA GGAGCTCCAG ACAGAACTGA AAAAGATGCA TGAAACTTCA 
GATCAGGAGA AAGTGCAACA CAAATCTGAG ATTGAAGCCA TGACCACTCA GTTAGCACAA 
AAGGCAGAAC AAGAAGGAAA ATTACAGCAG CAAGTGAAAG GATTAACCAG TGAACTGGAA 
ACTGAGAAAG GAAAAGTGTC TGATCTGCAG AAGTCCCACA AACTCCTTCA GGAGAAAGTG 
AATAGCCTTC AGTCAGATAT GTACGGGAAA GAGTCAGAAG TATCTGCAAT ACGGCAGGAT 
CTGAAAAATA CAGAGGAAAA GCTTATTCTT GCACAGGAAG AGCTAAAAAC AAACAGGAAT 
GAAGTCAATG GCCACAAAAA GCTTATACAA GAACTAACTG CTGTGAGAGG GACCTTGGAG 
CAGGATATAG CTAAAAATAA GAACTTGCTG AAGGAACGGG AAAAAATACT GCAGGAACTG 
CAGGAGCAGA AGGTCTTAAT AGAGAAGGAC ATAAGTGATG AAAAACTGAA AGTAACTAAT 
CTAAACTCCT TAAATGAGAA GCTTAAGGGA GAATTAATCC AGACTAGAGA AGAACTAAAG 
AGCATCAAAG ATGAATGCCA AAAGAAAACC AGCAGTTTTG AGGATGCCAA ACAGCTACTA 
ATCCAGCAGA AGCTGGAGTT GCAGGCCAAA CTTGACAGCT CAGCTTCAGT TCTACAACAG 
GAAAAGAATA GCCAACAGGC TCTGAAGGAG GACATCAAGA AAAAGCAAGA GGACTGGAAA 
AAACATAGCA CGGAGATGAG AGAGAAATTG TCTGCAGAAG CAAAAGAAAA AGAAGAACAA 
AAGAAGAAAC ATGAGGAGAA TGAGTCAAAG CTAAACATGC AGATTACAGC TTTAAATGAG 
AACCTGGCAA CGTTAAAGAA GGAATGGCAG AGCAGCCAAC GTAGGGTCGG TGAGCTGGAG 
AAGCAAACAG ACGACCTGCG GGGAGAGATT GCAGTATTAG AAGCAACAGT CCAAAACAAT 
CAGGACGACA GACGTGCTTT GTTAGAAAGG TGTCTTAAAA GTGAGGGTGA AATTGAAAAG 
CTCCAAGCCA AAATTTTCGA ACTGCGGAAA AAAGTAGATG AATCTTCTTA TGCAATGGAG 
GAACTTGGCA GGGAAAACCA ATCACTACAG ATAAAGCACA ACCAAGCACT CAGTAGGAAG 
TGGACAGAGG ACCACGAAGT GCAGAACTGC ATGTCATGTG GCAGAGGATT TTCTGTAACA 
ATAAGAAGGC ACCACTGTAG ACAGTGTGGA AATATCTTCT GTCATGAATG TTCCTCGAAG 
AATGCCTTAA CGCCCTCCTC CAAAAAAGCT GTGCGTGTTT GTGATACATG TTTTGGTGAC 
CTGCAAGGAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eea1 ( XM_004912917.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912974.2 Xenopus tropicalis early endosome antigen 1 (eea1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa32539
Clone ID Related Accession (Same CDS sequence)	XM_004912917.3
Accession Version	XM_004912917.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3912bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	early endosome antigen 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004912917.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTTTCA GAAGAGTTGA AGAAAGAGTT GGAAAATGTT CAAGGGCAAC TGAAAACACA 
ATTTCAGATG GGATGACCAC AATTGCAGCT ACAGAGCTTC AGTCTTTAGA ACAGAAGTTA 
GAAGAGGCCC AGACAGAAAT GTTTAATATA AAGCAAATGA AGGATTTATT TGAACAGAAA 
GCAGCTCAAC AGGCTACTGA AGTAGTTGAT TTAAAGGCAT CATATGATGA AGAGAAGAGT 
AACTTGGCAG CTGCACGGGA GAAAGTAAAA GGTTTAACAC AAGATCTGGA TTCAAAGTTA 
CACGTGATTG AAGACTTGAA GAATGAGCTG CTTCAGCGAC CTGGGATTGA AGATGTTGCT 
GTGTTGAAGA AGGAGCTTGT CCAGGTCCAG ACGCTGATGG ACAAAACAAC GCTAGATGCT 
GAAAATGAAT CAGAGAAGCT GAAAGATGAG TGCAGACAGC TGCAGGCACA AATCTCTAAC 
TCTGAGGTGA CTATTACTCA ACTGAAAGCT GAACTTGCTA AAGGCCCTCA AGAAGTTGCA 
GTTTATGTCC AGGAATTGCA GCAACTCAAA ACTGCTTTGA CTGAGTTAAA TCAAAAACAT 
GATAGTTTGA TGGAAAAATT TTTGAAGAAG GAATTGGATT ACACCCAGCT TGAGGAGAAA 
CAAAATGAGG AGTCCACTGC AAGGAAAAAC CTGCAAGTCC TTCTCAATCA GAAGGACGTG 
GAGTTCCAGC AATTGCAAGC TCGGTTCTCT GCAAGAGACA TGTCGCTGCA GACCGTTAAG 
GCTGAGGTGG GCGAGAAGGC TGAGACAAAT CAGAAACTAA AAGCGGAACT CTCAGAAGTC 
GAAACCAAAT ATCAGCATCT CAAAGCCGAA TTTAAGCAGC TTCAGCAGCA ACGGGAAGAG 
AAGGAGCAGC TTAGTTTGCA GCAGCAAAGT GAGATCAATC AGCTTCACAG CAAGTTACTG 
GAGACAGAGC GCCAACTAGG GGAAGCTCAT GGGAGGCTGA AGGAGCAGAG GCAGCTGTCT 
GCTGAGAAAC TGATGGACAA GGAGCAGCAG GTGGCTGATC TACAGCTGAA ACTCTCACGT 
GCTGAGGAGC AGCTCAAAGA AAAGACTTCC GCGTCTACTG ACTTGCAGCA TCAATTGGAA 
AAGATAAGGC AACAGCATCA GGAACAGACC AACCTCCAGC AGAATACCAC ATCCAAATTG 
AGAGAAGCCC AAAATGATTT AGAACAAGTC TTACGGCAGA TTGGGGACAA AGACCAGAAA 
ATCCAGAACC TTGAAGCACT ATTGGAGAAA AGCAAAAAGA ACATTTCTCT TCTTGAAAAA 
GAACGAGAAG ATTTATATGC CAAAATTCAA GCTGGTGAAG GAGAAACTGC AGTTCTAAAC 
CAACTTCAAG AGAAAAACCA CGCTTTGCAG GAACAGTTAA CCCAGCTTAC TGAGAAGTTA 
AAAAATCAAT CAGAAAGTCA CAAACAAGCC CAGGAAAACT TGCATGAGCA GGTTCAGGAG 
CAGAAGTCCC AACTCAGAGC CTCCCAAGAT CGTGTCCTGG CACTTGAGGC AACACTTAAT 
GAAGTCACCA GTCAGCTAAA TGAGAGCAAA GAGAAAATGG TTCAACTGGA TTTACAGATC 
AAAGCAAAAA CTGAACTTTT GTTAGCAGCA GAGGCAGCAA AGACAGCGCA AAGAGTAGAT 
CTTCAGAACC ACTTGGATAC AGCCCAAAAT GCACTGCAGG ATAAGCAACA GGAATTAAAC 
AAAGTGAACG CACAACTGGA ACAAGTAACA GCAAAGTTAA ATGACAAGCA GGAGTACAGC 
ATTCAACTGG AGGCGAACCT TAAGGAGTAC AAAGAGAAGC ACCTTAATCT GGAACAAAAG 
TCTGAAGCTC TGGAAGGGCA GCTTAAGAAA CTTGAAACAG ATCTAATCAG TGTGAAAGCA 
AACCGGGAAC AGACAACCCA GGAGCTGCAG CAGCAGAAGC AGCAGAGCGC TCAGATGGAA 
TTGAAATCTG CAGAGCTGAG CAAGCAGTTA GAAGAGGAGA AAGGACTGGT ATCAAGTATG 
AAACTGGAAC TACAGAAGAA AGTGGAGTCT CTCCAGCAGT CAAAGGAATT CATTTCAAAA 
CAAGATCAAG AAAGGAACAG TTTGAAGGAG AATATAGAAA AGTTAAAGCA AGACCTGGAG 
AAAATATCAG GCAAAATGCA AGATGCCGTG ACAAGCCTAG AGAAAACCAA ACTGGAGAGA 
GACGCTTTGC AAAAAGATCT TGCAGAGACC AAGAAGAAAT TATCTGAGAG CAGCAAATCT 
TTACAGGAAT CAAGAGATCA ACTGGAAAAA GAAAAACTGA AAGGAAAGAA AACAGTTGAA 
AACATGGAGA AGTCACAGGA AGAAGTGAAA TTGAATCTAC AAAGACAGGT GGATGCAACT 
GCAAAGGAGG TGAATGAGCT CAAGTCTATG CTGGAAAATA AGCAGGAGGA ATCCACCCAG 
ACAAAGGCTG AGTTGGATAA AGCCCGTGGC TACGTTGCAA AGCTGCACAG TGCTATCAAA 
GAAAAGGAGA AAGCTGAGCA GGAGCTCCAG ACAGAACTGA AAAAGATGCA TGAAACTTCA 
GATCAGGAGA AAGTGCAACA CAAATCTGAG ATTGAAGCCA TGACCACTCA GTTAGCACAA 
AAGGCAGAAC AAGAAGGAAA ATTACAGCAG CAAGTGAAAG GATTAACCAG TGAACTGGAA 
ACTGAGAAAG GAAAAGTGTC TGATCTGCAG AAGTCCCACA AACTCCTTCA GGAGAAAGTG 
AATAGCCTTC AGTCAGATAT GTACGGGAAA GAGTCAGAAG TATCTGCAAT ACGGCAGGAT 
CTGAAAAATA CAGAGGAAAA GCTTATTCTT GCACAGGAAG AGCTAAAAAC AAACAGGAAT 
GAAGTCAATG GCCACAAAAA GCTTATACAA GAACTAACTG CTGTGAGAGG GACCTTGGAG 
CAGGATATAG CTAAAAATAA GAACTTGCTG AAGGAACGGG AAAAAACACT GCAGGAACTG 
CAGGAGCAGA AGGTCTTAAT AGAGAAGGAC ATAAGTGATG AAAAACTGAA AGTAACTAAT 
CTAAACTCCT TAAATGAGAA GCTTAAGGGA GAATTAATCC AGACTAGAGA AGAACTAAAG 
AGCATCAAAG ATGAATGCCA AAAGAAAACC AGCAGTTTTG AGGATGCCAA ACAGCTACTA 
ATCCAGCAGA AGCTGGAGTT GCAGGCCAAA CTTGACAGCT CAGCTTCAGT TCTACAACAG 
GAAAAGAATA GCCAACAGGC TCTGAAGGAG GACATCAAGA AAAAGCAAGA GGACTGGAAA 
AAACATAGCA CGGAGATGAG AGAGAAATTG TCTGCAGAAG CAAAAGAAAA AGAAGAACAA 
AAGAAGAAAC ATGAGGAGAA TGAGTCAAAG CTAAACATGC AGATTACAGC TTTAAATGAG 
AACCTGGCAA CGTTAAAGAA GGAATGGCAG AGCAGCCAAC GTAGGGTCGG TGAGCTGGAG 
AAGCAAACAG ACGACCTGCG GGGAGAGATT GCAGTATTAG AAGCAACAGT CCAAAACAAT 
CAGGACGACA GACGTGCTTT GTTAGAAAGG TGTCTTAAAA GCGAGGGTGA AATTGAAAAG 
CTCCAAGCCA AAATTTTCGA ACTGCGGAAA AAAGTAGATG AATCTTCTTA TGCAATGGAG 
GAACTTGGCA GGGAAAACCA ATCACTACAG ATAAAGCACA ACCAAGCACT CAGTAGGAAG 
TGGACAGAGG ACCACGAAGT GCAGAACTGC ATGTCATGTG GCAGAGGATT TTCTGTAACA 
ATAAGAAGGC ACCACTGTAG ACAGTGTGGA AATATCTTCT GTCATGAATG TTCCTCGAAG 
AATGCCTTAA CGCCCTCCTC CAAAAAAGCT GTGCGTGTTT GTGATACATG TTTTGGTGAC 
CTGCAAGGAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912974.2
CDS	96..4007
Translation	MVFRRVEERVGKCSRATENTISDGMTTIAATELQSLEQKLEEAQTEMFNIKQMKDLFEQKAAQQATEVVDLKASYDEEKSNLAAAREKVKGLTQDLDSKLHVIEDLKNELLQRPGIEDVAVLKKELVQVQTLMDKTTLDAENESEKLKDECRQLQAQISNSEVTITQLKAELAKGPQEVAVYVQELQQLKTALTELNQKHDSLMEKFLKKELDYTQLEEKQNEESTARKNLQVLLNQKDVEFQQLQARFSARDMSLQTVKAEVGEKAETNQKLKAELSEVETKYQHLKAEFKQLQQQREEKEQLSLQQQSEINQLHSKLLETERQLGEAHGRLKEQRQLSAEKLMDKEQQVADLQLKLSRAEEQLKEKTSASTDLQHQLEKIRQQHQEQTNLQQNTTSKLREAQNDLEQVLRQIGDKDQKIQNLEALLEKSKKNISLLEKEREDLYAKIQAGEGETAVLNQLQEKNHALQEQLTQLTEKLKNQSESHKQAQENLHEQVQEQKSQLRASQDRVLALEATLNEVTSQLNESKEKMVQLDLQIKAKTELLLAAEAAKTAQRVDLQNHLDTAQNALQDKQQELNKVNAQLEQVTAKLNDKQEYSIQLEANLKEYKEKHLNLEQKSEALEGQLKKLETDLISVKANREQTTQELQQQKQQSAQMELKSAELSKQLEEEKGLVSSMKLELQKKVESLQQSKEFISKQDQERNSLKENIEKLKQDLEKISGKMQDAVTSLEKTKLERDALQKDLAETKKKLSESSKSLQESRDQLEKEKLKGKKTVENMEKSQEEVKLNLQRQVDATAKEVNELKSMLENKQEESTQTKAELDKARGYVAKLHSAIKEKEKAEQELQTELKKMHETSDQEKVQHKSEIEAMTTQLAQKAEQEGKLQQQVKGLTSELETEKGKVSDLQKSHKLLQEKVNSLQSDMYGKESEVSAIRQDLKNTEEKLILAQEELKTNRNEVNGHKKLIQELTAVRGTLEQDIAKNKNLLKEREKTLQELQEQKVLIEKDISDEKLKVTNLNSLNEKLKGELIQTREELKSIKDECQKKTSSFEDAKQLLIQQKLELQAKLDSSASVLQQEKNSQQALKEDIKKKQEDWKKHSTEMREKLSAEAKEKEEQKKKHEENESKLNMQITALNENLATLKKEWQSSQRRVGELEKQTDDLRGEIAVLEATVQNNQDDRRALLERCLKSEGEIEKLQAKIFELRKKVDESSYAMEELGRENQSLQIKHNQALSRKWTEDHEVQNCMSCGRGFSVTIRRHHCRQCGNIFCHECSSKNALTPSSKKAVRVCDTCFGDLQG

Target ORF information:

RefSeq Version	XM_004912917.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis early endosome antigen 1 (eea1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004912917.3

ORF Insert Sequence:

ATGGTTTTCA GAAGAGTTGA AGAAAGAGTT GGAAAATGTT CAAGGGCAAC TGAAAACACA 
ATTTCAGATG GGATGACCAC AATTGCAGCT ACAGAGCTTC AGTCTTTAGA ACAGAAGTTA 
GAAGAGGCCC AGACAGAAAT GTTTAATATA AAGCAAATGA AGGATTTATT TGAACAGAAA 
GCAGCTCAAC AGGCTACTGA AGTAGTTGAT TTAAAGGCAT CATATGATGA AGAGAAGAGT 
AACTTGGCAG CTGCACGGGA GAAAGTAAAA GGTTTAACAC AAGATCTGGA TTCAAAGTTA 
CACGTGATTG AAGACTTGAA GAATGAGCTG CTTCAGCGAC CTGGGATTGA AGATGTTGCT 
GTGTTGAAGA AGGAGCTTGT CCAGGTCCAG ACGCTGATGG ACAAAACAAC GCTAGATGCT 
GAAAATGAAT CAGAGAAGCT GAAAGATGAG TGCAGACAGC TGCAGGCACA AATCTCTAAC 
TCTGAGGTGA CTATTACTCA ACTGAAAGCT GAACTTGCTA AAGGCCCTCA AGAAGTTGCA 
GTTTATGTCC AGGAATTGCA GCAACTCAAA ACTGCTTTGA CTGAGTTAAA TCAAAAACAT 
GATAGTTTGA TGGAAAAATT TTTGAAGAAG GAATTGGATT ACACCCAGCT TGAGGAGAAA 
CAAAATGAGG AGTCCACTGC AAGGAAAAAC CTGCAAGTCC TTCTCAATCA GAAGGACGTG 
GAGTTCCAGC AATTGCAAGC TCGGTTCTCT GCAAGAGACA TGTCGCTGCA GACCGTTAAG 
GCTGAGGTGG GCGAGAAGGC TGAGACAAAT CAGAAACTAA AAGCGGAACT CTCAGAAGTC 
GAAACCAAAT ATCAGCATCT CAAAGCCGAA TTTAAGCAGC TTCAGCAGCA ACGGGAAGAG 
AAGGAGCAGC TTAGTTTGCA GCAGCAAAGT GAGATCAATC AGCTTCACAG CAAGTTACTG 
GAGACAGAGC GCCAACTAGG GGAAGCTCAT GGGAGGCTGA AGGAGCAGAG GCAGCTGTCT 
GCTGAGAAAC TGATGGACAA GGAGCAGCAG GTGGCTGATC TACAGCTGAA ACTCTCACGT 
GCTGAGGAGC AGCTCAAAGA AAAGACTTCC GCGTCTACTG ACTTGCAGCA TCAATTGGAA 
AAGATAAGGC AACAGCATCA GGAACAGACC AACCTCCAGC AGAATACCAC ATCCAAATTG 
AGAGAAGCCC AAAATGATTT AGAACAAGTC TTACGGCAGA TTGGGGACAA AGACCAGAAA 
ATCCAGAACC TTGAAGCACT ATTGGAGAAA AGCAAAAAGA ACATTTCTCT TCTTGAAAAA 
GAACGAGAAG ATTTATATGC CAAAATTCAA GCTGGTGAAG GAGAAACTGC AGTTCTAAAC 
CAACTTCAAG AGAAAAACCA CGCTTTGCAG GAACAGTTAA CCCAGCTTAC TGAGAAGTTA 
AAAAATCAAT CAGAAAGTCA CAAACAAGCC CAGGAAAACT TGCATGAGCA GGTTCAGGAG 
CAGAAGTCCC AACTCAGAGC CTCCCAAGAT CGTGTCCTGG CACTTGAGGC AACACTTAAT 
GAAGTCACCA GTCAGCTAAA TGAGAGCAAA GAGAAAATGG TTCAACTGGA TTTACAGATC 
AAAGCAAAAA CTGAACTTTT GTTAGCAGCA GAGGCAGCAA AGACAGCGCA AAGAGTAGAT 
CTTCAGAACC ACTTGGATAC AGCCCAAAAT GCACTGCAGG ATAAGCAACA GGAATTAAAC 
AAAGTGAACG CACAACTGGA ACAAGTAACA GCAAAGTTAA ATGACAAGCA GGAGTACAGC 
ATTCAACTGG AGGCGAACCT TAAGGAGTAC AAAGAGAAGC ACCTTAATCT GGAACAAAAG 
TCTGAAGCTC TGGAAGGGCA GCTTAAGAAA CTTGAAACAG ATCTAATCAG TGTGAAAGCA 
AACCGGGAAC AGACAACCCA GGAGCTGCAG CAGCAGAAGC AGCAGAGCGC TCAGATGGAA 
TTGAAATCTG CAGAGCTGAG CAAGCAGTTA GAAGAGGAGA AAGGACTGGT ATCAAGTATG 
AAACTGGAAC TACAGAAGAA AGTGGAGTCT CTCCAGCAGT CAAAGGAATT CATTTCAAAA 
CAAGATCAAG AAAGGAACAG TTTGAAGGAG AATATAGAAA AGTTAAAGCA AGACCTGGAG 
AAAATATCAG GCAAAATGCA AGATGCCGTG ACAAGCCTAG AGAAAACCAA ACTGGAGAGA 
GACGCTTTGC AAAAAGATCT TGCAGAGACC AAGAAGAAAT TATCTGAGAG CAGCAAATCT 
TTACAGGAAT CAAGAGATCA ACTGGAAAAA GAAAAACTGA AAGGAAAGAA AACAGTTGAA 
AACATGGAGA AGTCACAGGA AGAAGTGAAA TTGAATCTAC AAAGACAGGT GGATGCAACT 
GCAAAGGAGG TGAATGAGCT CAAGTCTATG CTGGAAAATA AGCAGGAGGA ATCCACCCAG 
ACAAAGGCTG AGTTGGATAA AGCCCGTGGC TACGTTGCAA AGCTGCACAG TGCTATCAAA 
GAAAAGGAGA AAGCTGAGCA GGAGCTCCAG ACAGAACTGA AAAAGATGCA TGAAACTTCA 
GATCAGGAGA AAGTGCAACA CAAATCTGAG ATTGAAGCCA TGACCACTCA GTTAGCACAA 
AAGGCAGAAC AAGAAGGAAA ATTACAGCAG CAAGTGAAAG GATTAACCAG TGAACTGGAA 
ACTGAGAAAG GAAAAGTGTC TGATCTGCAG AAGTCCCACA AACTCCTTCA GGAGAAAGTG 
AATAGCCTTC AGTCAGATAT GTACGGGAAA GAGTCAGAAG TATCTGCAAT ACGGCAGGAT 
CTGAAAAATA CAGAGGAAAA GCTTATTCTT GCACAGGAAG AGCTAAAAAC AAACAGGAAT 
GAAGTCAATG GCCACAAAAA GCTTATACAA GAACTAACTG CTGTGAGAGG GACCTTGGAG 
CAGGATATAG CTAAAAATAA GAACTTGCTG AAGGAACGGG AAAAAACACT GCAGGAACTG 
CAGGAGCAGA AGGTCTTAAT AGAGAAGGAC ATAAGTGATG AAAAACTGAA AGTAACTAAT 
CTAAACTCCT TAAATGAGAA GCTTAAGGGA GAATTAATCC AGACTAGAGA AGAACTAAAG 
AGCATCAAAG ATGAATGCCA AAAGAAAACC AGCAGTTTTG AGGATGCCAA ACAGCTACTA 
ATCCAGCAGA AGCTGGAGTT GCAGGCCAAA CTTGACAGCT CAGCTTCAGT TCTACAACAG 
GAAAAGAATA GCCAACAGGC TCTGAAGGAG GACATCAAGA AAAAGCAAGA GGACTGGAAA 
AAACATAGCA CGGAGATGAG AGAGAAATTG TCTGCAGAAG CAAAAGAAAA AGAAGAACAA 
AAGAAGAAAC ATGAGGAGAA TGAGTCAAAG CTAAACATGC AGATTACAGC TTTAAATGAG 
AACCTGGCAA CGTTAAAGAA GGAATGGCAG AGCAGCCAAC GTAGGGTCGG TGAGCTGGAG 
AAGCAAACAG ACGACCTGCG GGGAGAGATT GCAGTATTAG AAGCAACAGT CCAAAACAAT 
CAGGACGACA GACGTGCTTT GTTAGAAAGG TGTCTTAAAA GCGAGGGTGA AATTGAAAAG 
CTCCAAGCCA AAATTTTCGA ACTGCGGAAA AAAGTAGATG AATCTTCTTA TGCAATGGAG 
GAACTTGGCA GGGAAAACCA ATCACTACAG ATAAAGCACA ACCAAGCACT CAGTAGGAAG 
TGGACAGAGG ACCACGAAGT GCAGAACTGC ATGTCATGTG GCAGAGGATT TTCTGTAACA 
ATAAGAAGGC ACCACTGTAG ACAGTGTGGA AATATCTTCT GTCATGAATG TTCCTCGAAG 
AATGCCTTAA CGCCCTCCTC CAAAAAAGCT GTGCGTGTTT GTGATACATG TTTTGGTGAC 
CTGCAAGGAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eea1 ( XM_002935315.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002935361.1 Xenopus tropicalis early endosome antigen 1 (eea1), transcript variant X1, mRNA.

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CloneID	OXa07736
Clone ID Related Accession (Same CDS sequence)	XM_002935315.4
Accession Version	XM_002935315.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4209bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	early endosome antigen 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002935315.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGGA GGATTTTACA GCTGACTCCT GGCAGAGTTG GCTCACAGAA CTCTGAATCT 
GACTCATCAG CCACTCCAGG AAATGCTGAA AGCTCATCAG AGGGTTTCAT TTGTCCGCTG 
TGTATGAAAT CCCATGCAAC AGCTGAAGAA CTTTTTGTGC ACTATGAAGC ATCTCACGAA 
CCTGCCAATG ACCCTGGTCA AGGAGGACAA ACCAATCTGT CGCCAAAAAG AGATGAAGTG 
CTCTTACTTC GTCAGGAAGT CAAAGATTTA CAGACCTCAT TAAAGGAAGA AAAATGGTTT 
TCAGAAGAGT TGAAGAAAGA GTTGGAAAAT GTTCAAGGGC AACTGAAGCA AAACACAATT 
TCAGATGGGA TGACCACAAT TGCAGCTACA GAGCTTCAGT CTTTAGAACA GAAGTTAGAA 
GAGGCCCAGA CAGAAATGTT TAATATAAAG CAAATGAAGG ATTTATTTGA ACAGAAAGCA 
GCTCAACAGG CTACTGAAGT AGTTGATTTA AAGGCATCAT ATGATGAAGA GAAGAGTAAC 
TTGGCAGCTG CACGGGAGAA AGTAAAAGGT TTAACACAAG ATCTGGATTC AAAGTTACAC 
GTGATTGAAG ACTTGAAGAA TGAGCTGCTT CAGCGACCTG GGATTGAAGA TGTTGCTGTG 
TTGAAGAAGG AGCTTGTCCA GGTCCAGACG CTGATGGACA AAACAACGCT AGATGCTGAA 
AATGAATCAG AGAAGCTGAA AGATGAGTGC AGACAGCTGC AGGCACAAAT CTCTAACTCT 
GAGGTGACTA TTACTCAACT GAAAGCTGAA CTTGCTAAAG GCCCTCAAGA AGTTGCAGTT 
TATGTCCAGG AATTGCAGCA ACTCAAAACT GCTTTGACTG AGTTAAATCA AAAACATGAT 
AGTTTGATGG AAAAATTTTT GAAGAAGGAA TTGGATTACA CCCAGCTTGA GGAGAAACAA 
AATGAGGAGT CCACTGCAAG GAAAAACCTG CAAGTCCTTC TCAATCAGAA GGACGTGGAG 
TTCCAGCAAT TGCAAGCTCG GTTCTCTGCA AGAGACATGT CGCTGCAGAC CGTTAAGGCT 
GAGGTGGGCG AGAAGGCTGA GACAAATCAG AAACTAAAAG CGGAACTCTC AGAAGTCGAA 
ACCAAATATC AGCATCTCAA AGCCGAATTT AAGCAGCTTC AGCAGCAACG GGAAGAGAAG 
GAGCAGCTTA GTTTGCAGCA GCAAAGTGAG ATCAATCAGC TTCACAGCAA GTTACTGGAG 
ACAGAGCGCC AACTAGGGGA AGCTCATGGG AGGCTGAAGG AGCAGAGGCA GCTGTCTGCT 
GAGAAACTGA TGGACAAGGA GCAGCAGGTG GCTGATCTAC AGCTGAAACT CTCACGTGCT 
GAGGAGCAGC TCAAAGAAAA GACTTCCGCG TCTACTGACT TGCAGCATCA ATTGGAAAAG 
ATAAGGCAAC AGCATCAGGA ACAGACCAAC CTCCAGCAGA ATACCACATC CAAATTGAGA 
GAAGCCCAAA ATGATTTAGA ACAAGTCTTA CGGCAGATTG GGGACAAAGA CCAGAAAATC 
CAGAACCTTG AAGCACTATT GGAGAAAAGC AAAAAGAACA TTTCTCTTCT TGAAAAAGAA 
CGAGAAGATT TATATGCCAA AATTCAAGCT GGTGAAGGAG AAACTGCAGT TCTAAACCAA 
CTTCAAGAGA AAAACCACGC TTTGCAGGAA CAGTTAACCC AGCTTACTGA GAAGTTAAAA 
AATCAATCAG AAAGTCACAA ACAAGCCCAG GAAAACTTGC ATGAGCAGGT TCAGGAGCAG 
AAGTCCCAAC TCAGAGCCTC CCAAGATCGT GTCCTGGCAC TTGAGGCAAC ACTTAATGAA 
GTCACCAGTC AGCTAAATGA GAGCAAAGAG AAAATGGTTC AACTGGATTT ACAGATCAAA 
GCAAAAACTG AACTTTTGTT AGCAGCAGAG GCAGCAAAGA CAGCGCAAAG AGTAGATCTT 
CAGAACCACT TGGATACAGC CCAAAATGCA CTGCAGGATA AGCAACAGGA ATTAAACAAA 
GTGAACGCAC AACTGGAACA AGTAACAGCA AAGTTAAATG ACAAGCAGGA GTACAGCATT 
CAACTGGAGG CGAACCTTAA GGAGTACAAA GAGAAGCACC TTAATCTGGA ACAAAAGTCT 
GAAGCTCTGG AAGGGCAGCT TAAGAAACTT GAAACAGATC TAATCAGTGT GAAAGCAAAC 
CGGGAACAGA CAACCCAGGA GCTGCAGCAG CAGAAGCAGC AGAGCGCTCA GATGGAATTG 
AAATCTGCAG AGCTGAGCAA GCAGTTAGAA GAGGAGAAAG GACTGGTATC AAGTATGAAA 
CTGGAACTAC AGAAGAAAGT GGAGTCTCTC CAGCAGTCAA AGGAATTCAT TTCAAAACAA 
GATCAAGAAA GGAACAGTTT GAAGGAGAAT ATAGAAAAGT TAAAGCAAGA CCTGGAGAAA 
ATATCAGGCA AAATGCAAGA TGCCGTGACA AGCCTAGAGA AAACCAAACT GGAGAGAGAC 
GCTTTGCAAA AAGATCTTGC AGAGACCAAG AAGAAATTAT CTGAGAGCAG CAAATCTTTA 
CAGGAATCAA GAGATCAACT GGAAAAAGAA AAACTGAAAG GAAAGAAAAC AGTTGAAAAC 
ATGGAGAAGT CACAGGAAGA AGTGAAATTG AATCTACAAA GACAGGTGGA TGCAACTGCA 
AAGGAGGTGA ATGAGCTCAA GTCTATGCTG GAAAATAAGC AGGAGGAATC CACCCAGACA 
AAGGCTGAGT TGGATAAAGC CCGTGGCTAC GTTGCAAAGC TGCACAGTGC TATCAAAGAA 
AAGGAGAAAG CTGAGCAGGA GCTCCAGACA GAACTGAAAA AGATGCATGA AACTTCAGAT 
CAGGAGAAAG TGCAACACAA ATCTGAGATT GAAGCCATGA CCACTCAGTT AGCACAAAAG 
GCAGAACAAG AAGGAAAATT ACAGCAGCAA GTGAAAGGAT TAACCAGTGA ACTGGAAACT 
GAGAAAGGAA AAGTGTCTGA TCTGCAGAAG TCCCACAAAC TCCTTCAGGA GAAAGTGAAT 
AGCCTTCAGT CAGATATGTA CGGGAAAGAG TCAGAAGTAT CTGCAATACG GCAGGATCTG 
AAAAATACAG AGGAAAAGCT TATTCTTGCA CAGGAAGAGC TAAAAACAAA CAGGAATGAA 
GTCAATGGCC ACAAAAAGCT TATACAAGAA CTAACTGCTG TGAGAGGGAC CTTGGAGCAG 
GATATAGCTA AAAATAAGAA CTTGCTGAAG GAACGGGAAA AAACACTGCA GGAACTGCAG 
GAGCAGAAGG TCTTAATAGA GAAGGACATA AGTGATGAAA AACTGAAAGT AACTAATCTA 
AACTCCTTAA ATGAGAAGCT TAAGGGAGAA TTAATCCAGA CTAGAGAAGA ACTAAAGAGC 
ATCAAAGATG AATGCCAAAA GAAAACCAGC AGTTTTGAGG ATGCCAAACA GCTACTAATC 
CAGCAGAAGC TGGAGTTGCA GGCCAAACTT GACAGCTCAG CTTCAGTTCT ACAACAGGAA 
AAGAATAGCC AACAGGCTCT GAAGGAGGAC ATCAAGAAAA AGCAAGAGGA CTGGAAAAAA 
CATAGCACGG AGATGAGAGA GAAATTGTCT GCAGAAGCAA AAGAAAAAGA AGAACAAAAG 
AAGAAACATG AGGAGAATGA GTCAAAGCTA AACATGCAGA TTACAGCTTT AAATGAGAAC 
CTGGCAACGT TAAAGAAGGA ATGGCAGAGC AGCCAACGTA GGGTCGGTGA GCTGGAGAAG 
CAAACAGACG ACCTGCGGGG AGAGATTGCA GTATTAGAAG CAACAGTCCA AAACAATCAG 
GACGACAGAC GTGCTTTGTT AGAAAGGTGT CTTAAAAGCG AGGGTGAAAT TGAAAAGCTC 
CAAGCCAAAA TTTTCGAACT GCGGAAAAAA GTAGATGAAT CTTCTTATGC AATGGAGGAA 
CTTGGCAGGG AAAACCAATC ACTACAGATA AAGCACAACC AAGCACTCAG TAGGAAGTGG 
ACAGAGGACC ACGAAGTGCA GAACTGCATG TCATGTGGCA GAGGATTTTC TGTAACAATA 
AGAAGGCACC ACTGTAGACA GTGTGGAAAT ATCTTCTGTC ATGAATGTTC CTCGAAGAAT 
GCCTTAACGC CCTCCTCCAA AAAAGCTGTG CGTGTTTGTG ATACATGTTT TGGTGACCTG 
CAAGGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002935361.1
CDS	265..4473
Translation	MFRRILQLTPGRVGSQNSESDSSATPGNAESSSEGFICPLCMKSHATAEELFVHYEASHEPANDPGQGGQTNLSPKRDEVLLLRQEVKDLQTSLKEEKWFSEELKKELENVQGQLKQNTISDGMTTIAATELQSLEQKLEEAQTEMFNIKQMKDLFEQKAAQQATEVVDLKASYDEEKSNLAAAREKVKGLTQDLDSKLHVIEDLKNELLQRPGIEDVAVLKKELVQVQTLMDKTTLDAENESEKLKDECRQLQAQISNSEVTITQLKAELAKGPQEVAVYVQELQQLKTALTELNQKHDSLMEKFLKKELDYTQLEEKQNEESTARKNLQVLLNQKDVEFQQLQARFSARDMSLQTVKAEVGEKAETNQKLKAELSEVETKYQHLKAEFKQLQQQREEKEQLSLQQQSEINQLHSKLLETERQLGEAHGRLKEQRQLSAEKLMDKEQQVADLQLKLSRAEEQLKEKTSASTDLQHQLEKIRQQHQEQTNLQQNTTSKLREAQNDLEQVLRQIGDKDQKIQNLEALLEKSKKNISLLEKEREDLYAKIQAGEGETAVLNQLQEKNHALQEQLTQLTEKLKNQSESHKQAQENLHEQVQEQKSQLRASQDRVLALEATLNEVTSQLNESKEKMVQLDLQIKAKTELLLAAEAAKTAQRVDLQNHLDTAQNALQDKQQELNKVNAQLEQVTAKLNDKQEYSIQLEANLKEYKEKHLNLEQKSEALEGQLKKLETDLISVKANREQTTQELQQQKQQSAQMELKSAELSKQLEEEKGLVSSMKLELQKKVESLQQSKEFISKQDQERNSLKENIEKLKQDLEKISGKMQDAVTSLEKTKLERDALQKDLAETKKKLSESSKSLQESRDQLEKEKLKGKKTVENMEKSQEEVKLNLQRQVDATAKEVNELKSMLENKQEESTQTKAELDKARGYVAKLHSAIKEKEKAEQELQTELKKMHETSDQEKVQHKSEIEAMTTQLAQKAEQEGKLQQQVKGLTSELETEKGKVSDLQKSHKLLQEKVNSLQSDMYGKESEVSAIRQDLKNTEEKLILAQEELKTNRNEVNGHKKLIQELTAVRGTLEQDIAKNKNLLKEREKTLQELQEQKVLIEKDISDEKLKVTNLNSLNEKLKGELIQTREELKSIKDECQKKTSSFEDAKQLLIQQKLELQAKLDSSASVLQQEKNSQQALKEDIKKKQEDWKKHSTEMREKLSAEAKEKEEQKKKHEENESKLNMQITALNENLATLKKEWQSSQRRVGELEKQTDDLRGEIAVLEATVQNNQDDRRALLERCLKSEGEIEKLQAKIFELRKKVDESSYAMEELGRENQSLQIKHNQALSRKWTEDHEVQNCMSCGRGFSVTIRRHHCRQCGNIFCHECSSKNALTPSSKKAVRVCDTCFGDLQG

Target ORF information:

RefSeq Version	XM_002935315.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis early endosome antigen 1 (eea1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002935315.4

ORF Insert Sequence:

ATGTTCAGGA GGATTTTACA GCTGACTCCT GGCAGAGTTG GCTCACAGAA CTCTGAATCT 
GACTCATCAG CCACTCCAGG AAATGCTGAA AGCTCATCAG AGGGTTTCAT TTGTCCGCTG 
TGTATGAAAT CCCATGCAAC AGCTGAAGAA CTTTTTGTGC ACTATGAAGC ATCTCACGAA 
CCTGCCAATG ACCCTGGTCA AGGAGGACAA ACCAATCTGT CGCCAAAAAG AGATGAAGTG 
CTCTTACTTC GTCAGGAAGT CAAAGATTTA CAGACCTCAT TAAAGGAAGA AAAATGGTTT 
TCAGAAGAGT TGAAGAAAGA GTTGGAAAAT GTTCAAGGGC AACTGAAGCA AAACACAATT 
TCAGATGGGA TGACCACAAT TGCAGCTACA GAGCTTCAGT CTTTAGAACA GAAGTTAGAA 
GAGGCCCAGA CAGAAATGTT TAATATAAAG CAAATGAAGG ATTTATTTGA ACAGAAAGCA 
GCTCAACAGG CTACTGAAGT AGTTGATTTA AAGGCATCAT ATGATGAAGA GAAGAGTAAC 
TTGGCAGCTG CACGGGAGAA AGTAAAAGGT TTAACACAAG ATCTGGATTC AAAGTTACAC 
GTGATTGAAG ACTTGAAGAA TGAGCTGCTT CAGCGACCTG GGATTGAAGA TGTTGCTGTG 
TTGAAGAAGG AGCTTGTCCA GGTCCAGACG CTGATGGACA AAACAACGCT AGATGCTGAA 
AATGAATCAG AGAAGCTGAA AGATGAGTGC AGACAGCTGC AGGCACAAAT CTCTAACTCT 
GAGGTGACTA TTACTCAACT GAAAGCTGAA CTTGCTAAAG GCCCTCAAGA AGTTGCAGTT 
TATGTCCAGG AATTGCAGCA ACTCAAAACT GCTTTGACTG AGTTAAATCA AAAACATGAT 
AGTTTGATGG AAAAATTTTT GAAGAAGGAA TTGGATTACA CCCAGCTTGA GGAGAAACAA 
AATGAGGAGT CCACTGCAAG GAAAAACCTG CAAGTCCTTC TCAATCAGAA GGACGTGGAG 
TTCCAGCAAT TGCAAGCTCG GTTCTCTGCA AGAGACATGT CGCTGCAGAC CGTTAAGGCT 
GAGGTGGGCG AGAAGGCTGA GACAAATCAG AAACTAAAAG CGGAACTCTC AGAAGTCGAA 
ACCAAATATC AGCATCTCAA AGCCGAATTT AAGCAGCTTC AGCAGCAACG GGAAGAGAAG 
GAGCAGCTTA GTTTGCAGCA GCAAAGTGAG ATCAATCAGC TTCACAGCAA GTTACTGGAG 
ACAGAGCGCC AACTAGGGGA AGCTCATGGG AGGCTGAAGG AGCAGAGGCA GCTGTCTGCT 
GAGAAACTGA TGGACAAGGA GCAGCAGGTG GCTGATCTAC AGCTGAAACT CTCACGTGCT 
GAGGAGCAGC TCAAAGAAAA GACTTCCGCG TCTACTGACT TGCAGCATCA ATTGGAAAAG 
ATAAGGCAAC AGCATCAGGA ACAGACCAAC CTCCAGCAGA ATACCACATC CAAATTGAGA 
GAAGCCCAAA ATGATTTAGA ACAAGTCTTA CGGCAGATTG GGGACAAAGA CCAGAAAATC 
CAGAACCTTG AAGCACTATT GGAGAAAAGC AAAAAGAACA TTTCTCTTCT TGAAAAAGAA 
CGAGAAGATT TATATGCCAA AATTCAAGCT GGTGAAGGAG AAACTGCAGT TCTAAACCAA 
CTTCAAGAGA AAAACCACGC TTTGCAGGAA CAGTTAACCC AGCTTACTGA GAAGTTAAAA 
AATCAATCAG AAAGTCACAA ACAAGCCCAG GAAAACTTGC ATGAGCAGGT TCAGGAGCAG 
AAGTCCCAAC TCAGAGCCTC CCAAGATCGT GTCCTGGCAC TTGAGGCAAC ACTTAATGAA 
GTCACCAGTC AGCTAAATGA GAGCAAAGAG AAAATGGTTC AACTGGATTT ACAGATCAAA 
GCAAAAACTG AACTTTTGTT AGCAGCAGAG GCAGCAAAGA CAGCGCAAAG AGTAGATCTT 
CAGAACCACT TGGATACAGC CCAAAATGCA CTGCAGGATA AGCAACAGGA ATTAAACAAA 
GTGAACGCAC AACTGGAACA AGTAACAGCA AAGTTAAATG ACAAGCAGGA GTACAGCATT 
CAACTGGAGG CGAACCTTAA GGAGTACAAA GAGAAGCACC TTAATCTGGA ACAAAAGTCT 
GAAGCTCTGG AAGGGCAGCT TAAGAAACTT GAAACAGATC TAATCAGTGT GAAAGCAAAC 
CGGGAACAGA CAACCCAGGA GCTGCAGCAG CAGAAGCAGC AGAGCGCTCA GATGGAATTG 
AAATCTGCAG AGCTGAGCAA GCAGTTAGAA GAGGAGAAAG GACTGGTATC AAGTATGAAA 
CTGGAACTAC AGAAGAAAGT GGAGTCTCTC CAGCAGTCAA AGGAATTCAT TTCAAAACAA 
GATCAAGAAA GGAACAGTTT GAAGGAGAAT ATAGAAAAGT TAAAGCAAGA CCTGGAGAAA 
ATATCAGGCA AAATGCAAGA TGCCGTGACA AGCCTAGAGA AAACCAAACT GGAGAGAGAC 
GCTTTGCAAA AAGATCTTGC AGAGACCAAG AAGAAATTAT CTGAGAGCAG CAAATCTTTA 
CAGGAATCAA GAGATCAACT GGAAAAAGAA AAACTGAAAG GAAAGAAAAC AGTTGAAAAC 
ATGGAGAAGT CACAGGAAGA AGTGAAATTG AATCTACAAA GACAGGTGGA TGCAACTGCA 
AAGGAGGTGA ATGAGCTCAA GTCTATGCTG GAAAATAAGC AGGAGGAATC CACCCAGACA 
AAGGCTGAGT TGGATAAAGC CCGTGGCTAC GTTGCAAAGC TGCACAGTGC TATCAAAGAA 
AAGGAGAAAG CTGAGCAGGA GCTCCAGACA GAACTGAAAA AGATGCATGA AACTTCAGAT 
CAGGAGAAAG TGCAACACAA ATCTGAGATT GAAGCCATGA CCACTCAGTT AGCACAAAAG 
GCAGAACAAG AAGGAAAATT ACAGCAGCAA GTGAAAGGAT TAACCAGTGA ACTGGAAACT 
GAGAAAGGAA AAGTGTCTGA TCTGCAGAAG TCCCACAAAC TCCTTCAGGA GAAAGTGAAT 
AGCCTTCAGT CAGATATGTA CGGGAAAGAG TCAGAAGTAT CTGCAATACG GCAGGATCTG 
AAAAATACAG AGGAAAAGCT TATTCTTGCA CAGGAAGAGC TAAAAACAAA CAGGAATGAA 
GTCAATGGCC ACAAAAAGCT TATACAAGAA CTAACTGCTG TGAGAGGGAC CTTGGAGCAG 
GATATAGCTA AAAATAAGAA CTTGCTGAAG GAACGGGAAA AAACACTGCA GGAACTGCAG 
GAGCAGAAGG TCTTAATAGA GAAGGACATA AGTGATGAAA AACTGAAAGT AACTAATCTA 
AACTCCTTAA ATGAGAAGCT TAAGGGAGAA TTAATCCAGA CTAGAGAAGA ACTAAAGAGC 
ATCAAAGATG AATGCCAAAA GAAAACCAGC AGTTTTGAGG ATGCCAAACA GCTACTAATC 
CAGCAGAAGC TGGAGTTGCA GGCCAAACTT GACAGCTCAG CTTCAGTTCT ACAACAGGAA 
AAGAATAGCC AACAGGCTCT GAAGGAGGAC ATCAAGAAAA AGCAAGAGGA CTGGAAAAAA 
CATAGCACGG AGATGAGAGA GAAATTGTCT GCAGAAGCAA AAGAAAAAGA AGAACAAAAG 
AAGAAACATG AGGAGAATGA GTCAAAGCTA AACATGCAGA TTACAGCTTT AAATGAGAAC 
CTGGCAACGT TAAAGAAGGA ATGGCAGAGC AGCCAACGTA GGGTCGGTGA GCTGGAGAAG 
CAAACAGACG ACCTGCGGGG AGAGATTGCA GTATTAGAAG CAACAGTCCA AAACAATCAG 
GACGACAGAC GTGCTTTGTT AGAAAGGTGT CTTAAAAGCG AGGGTGAAAT TGAAAAGCTC 
CAAGCCAAAA TTTTCGAACT GCGGAAAAAA GTAGATGAAT CTTCTTATGC AATGGAGGAA 
CTTGGCAGGG AAAACCAATC ACTACAGATA AAGCACAACC AAGCACTCAG TAGGAAGTGG 
ACAGAGGACC ACGAAGTGCA GAACTGCATG TCATGTGGCA GAGGATTTTC TGTAACAATA 
AGAAGGCACC ACTGTAGACA GTGTGGAAAT ATCTTCTGTC ATGAATGTTC CTCGAAGAAT 
GCCTTAACGC CCTCCTCCAA AAAAGCTGTG CGTGTTTGTG ATACATGTTT TGGTGACCTG 
CAAGGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eea1 ( XM_012959889.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012815343.1 Xenopus tropicalis early endosome antigen 1 (eea1), transcript variant X2, mRNA.

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CloneID	OXa32538
Clone ID Related Accession (Same CDS sequence)	XM_012959889.2 , XM_018092239.1
Accession Version	XM_012959889.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4086bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	early endosome antigen 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959889.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCC ATGCAACAGC TGAAGAACTT TTTGTGCACT ATGAAGCATC TCACGAACCT 
GCCAATGACC CTGGTCAAGG AGGACAAACC AATCTGTCGC CAAAAAGAGA TGAAGTGCTC 
TTACTTCGTC AGGAAGTCAA AGATTTACAG ACCTCATTAA AGGAAGAAAA ATGGTTTTCA 
GAAGAGTTGA AGAAAGAGTT GGAAAATGTT CAAGGGCAAC TGAAGCAAAA CACAATTTCA 
GATGGGATGA CCACAATTGC AGCTACAGAG CTTCAGTCTT TAGAACAGAA GTTAGAAGAG 
GCCCAGACAG AAATGTTTAA TATAAAGCAA ATGAAGGATT TATTTGAACA GAAAGCAGCT 
CAACAGGCTA CTGAAGTAGT TGATTTAAAG GCATCATATG ATGAAGAGAA GAGTAACTTG 
GCAGCTGCAC GGGAGAAAGT AAAAGGTTTA ACACAAGATC TGGATTCAAA GTTACACGTG 
ATTGAAGACT TGAAGAATGA GCTGCTTCAG CGACCTGGGA TTGAAGATGT TGCTGTGTTG 
AAGAAGGAGC TTGTCCAGGT CCAGACGCTG ATGGACAAAA CAACGCTAGA TGCTGAAAAT 
GAATCAGAGA AGCTGAAAGA TGAGTGCAGA CAGCTGCAGG CACAAATCTC TAACTCTGAG 
GTGACTATTA CTCAACTGAA AGCTGAACTT GCTAAAGGCC CTCAAGAAGT TGCAGTTTAT 
GTCCAGGAAT TGCAGCAACT CAAAACTGCT TTGACTGAGT TAAATCAAAA ACATGATAGT 
TTGATGGAAA AATTTTTGAA GAAGGAATTG GATTACACCC AGCTTGAGGA GAAACAAAAT 
GAGGAGTCCA CTGCAAGGAA AAACCTGCAA GTCCTTCTCA ATCAGAAGGA CGTGGAGTTC 
CAGCAATTGC AAGCTCGGTT CTCTGCAAGA GACATGTCGC TGCAGACCGT TAAGGCTGAG 
GTGGGCGAGA AGGCTGAGAC AAATCAGAAA CTAAAAGCGG AACTCTCAGA AGTCGAAACC 
AAATATCAGC ATCTCAAAGC CGAATTTAAG CAGCTTCAGC AGCAACGGGA AGAGAAGGAG 
CAGCTTAGTT TGCAGCAGCA AAGTGAGATC AATCAGCTTC ACAGCAAGTT ACTGGAGACA 
GAGCGCCAAC TAGGGGAAGC TCATGGGAGG CTGAAGGAGC AGAGGCAGCT GTCTGCTGAG 
AAACTGATGG ACAAGGAGCA GCAGGTGGCT GATCTACAGC TGAAACTCTC ACGTGCTGAG 
GAGCAGCTCA AAGAAAAGAC TTCCGCGTCT ACTGACTTGC AGCATCAATT GGAAAAGATA 
AGGCAACAGC ATCAGGAACA GACCAACCTC CAGCAGAATA CCACATCCAA ATTGAGAGAA 
GCCCAAAATG ATTTAGAACA AGTCTTACGG CAGATTGGGG ACAAAGACCA GAAAATCCAG 
AACCTTGAAG CACTATTGGA GAAAAGCAAA AAGAACATTT CTCTTCTTGA AAAAGAACGA 
GAAGATTTAT ATGCCAAAAT TCAAGCTGGT GAAGGAGAAA CTGCAGTTCT AAACCAACTT 
CAAGAGAAAA ACCACGCTTT GCAGGAACAG TTAACCCAGC TTACTGAGAA GTTAAAAAAT 
CAATCAGAAA GTCACAAACA AGCCCAGGAA AACTTGCATG AGCAGGTTCA GGAGCAGAAG 
TCCCAACTCA GAGCCTCCCA AGATCGTGTC CTGGCACTTG AGGCAACACT TAATGAAGTC 
ACCAGTCAGC TAAATGAGAG CAAAGAGAAA ATGGTTCAAC TGGATTTACA GATCAAAGCA 
AAAACTGAAC TTTTGTTAGC AGCAGAGGCA GCAAAGACAG CGCAAAGAGT AGATCTTCAG 
AACCACTTGG ATACAGCCCA AAATGCACTG CAGGATAAGC AACAGGAATT AAACAAAGTG 
AACGCACAAC TGGAACAAGT AACAGCAAAG TTAAATGACA AGCAGGAGTA CAGCATTCAA 
CTGGAGGCGA ACCTTAAGGA GTACAAAGAG AAGCACCTTA ATCTGGAACA AAAGTCTGAA 
GCTCTGGAAG GGCAGCTTAA GAAACTTGAA ACAGATCTAA TCAGTGTGAA AGCAAACCGG 
GAACAGACAA CCCAGGAGCT GCAGCAGCAG AAGCAGCAGA GCGCTCAGAT GGAATTGAAA 
TCTGCAGAGC TGAGCAAGCA GTTAGAAGAG GAGAAAGGAC TGGTATCAAG TATGAAACTG 
GAACTACAGA AGAAAGTGGA GTCTCTCCAG CAGTCAAAGG AATTCATTTC AAAACAAGAT 
CAAGAAAGGA ACAGTTTGAA GGAGAATATA GAAAAGTTAA AGCAAGACCT GGAGAAAATA 
TCAGGCAAAA TGCAAGATGC CGTGACAAGC CTAGAGAAAA CCAAACTGGA GAGAGACGCT 
TTGCAAAAAG ATCTTGCAGA GACCAAGAAG AAATTATCTG AGAGCAGCAA ATCTTTACAG 
GAATCAAGAG ATCAACTGGA AAAAGAAAAA CTGAAAGGAA AGAAAACAGT TGAAAACATG 
GAGAAGTCAC AGGAAGAAGT GAAATTGAAT CTACAAAGAC AGGTGGATGC AACTGCAAAG 
GAGGTGAATG AGCTCAAGTC TATGCTGGAA AATAAGCAGG AGGAATCCAC CCAGACAAAG 
GCTGAGTTGG ATAAAGCCCG TGGCTACGTT GCAAAGCTGC ACAGTGCTAT CAAAGAAAAG 
GAGAAAGCTG AGCAGGAGCT CCAGACAGAA CTGAAAAAGA TGCATGAAAC TTCAGATCAG 
GAGAAAGTGC AACACAAATC TGAGATTGAA GCCATGACCA CTCAGTTAGC ACAAAAGGCA 
GAACAAGAAG GAAAATTACA GCAGCAAGTG AAAGGATTAA CCAGTGAACT GGAAACTGAG 
AAAGGAAAAG TGTCTGATCT GCAGAAGTCC CACAAACTCC TTCAGGAGAA AGTGAATAGC 
CTTCAGTCAG ATATGTACGG GAAAGAGTCA GAAGTATCTG CAATACGGCA GGATCTGAAA 
AATACAGAGG AAAAGCTTAT TCTTGCACAG GAAGAGCTAA AAACAAACAG GAATGAAGTC 
AATGGCCACA AAAAGCTTAT ACAAGAACTA ACTGCTGTGA GAGGGACCTT GGAGCAGGAT 
ATAGCTAAAA ATAAGAACTT GCTGAAGGAA CGGGAAAAAA CACTGCAGGA ACTGCAGGAG 
CAGAAGGTCT TAATAGAGAA GGACATAAGT GATGAAAAAC TGAAAGTAAC TAATCTAAAC 
TCCTTAAATG AGAAGCTTAA GGGAGAATTA ATCCAGACTA GAGAAGAACT AAAGAGCATC 
AAAGATGAAT GCCAAAAGAA AACCAGCAGT TTTGAGGATG CCAAACAGCT ACTAATCCAG 
CAGAAGCTGG AGTTGCAGGC CAAACTTGAC AGCTCAGCTT CAGTTCTACA ACAGGAAAAG 
AATAGCCAAC AGGCTCTGAA GGAGGACATC AAGAAAAAGC AAGAGGACTG GAAAAAACAT 
AGCACGGAGA TGAGAGAGAA ATTGTCTGCA GAAGCAAAAG AAAAAGAAGA ACAAAAGAAG 
AAACATGAGG AGAATGAGTC AAAGCTAAAC ATGCAGATTA CAGCTTTAAA TGAGAACCTG 
GCAACGTTAA AGAAGGAATG GCAGAGCAGC CAACGTAGGG TCGGTGAGCT GGAGAAGCAA 
ACAGACGACC TGCGGGGAGA GATTGCAGTA TTAGAAGCAA CAGTCCAAAA CAATCAGGAC 
GACAGACGTG CTTTGTTAGA AAGGTGTCTT AAAAGCGAGG GTGAAATTGA AAAGCTCCAA 
GCCAAAATTT TCGAACTGCG GAAAAAAGTA GATGAATCTT CTTATGCAAT GGAGGAACTT 
GGCAGGGAAA ACCAATCACT ACAGATAAAG CACAACCAAG CACTCAGTAG GAAGTGGACA 
GAGGACCACG AAGTGCAGAA CTGCATGTCA TGTGGCAGAG GATTTTCTGT AACAATAAGA 
AGGCACCACT GTAGACAGTG TGGAAATATC TTCTGTCATG AATGTTCCTC GAAGAATGCC 
TTAACGCCCT CCTCCAAAAA AGCTGTGCGT GTTTGTGATA CATGTTTTGG TGACCTGCAA 
GGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012815343.1
CDS	1089..5174
Translation	MKSHATAEELFVHYEASHEPANDPGQGGQTNLSPKRDEVLLLRQEVKDLQTSLKEEKWFSEELKKELENVQGQLKQNTISDGMTTIAATELQSLEQKLEEAQTEMFNIKQMKDLFEQKAAQQATEVVDLKASYDEEKSNLAAAREKVKGLTQDLDSKLHVIEDLKNELLQRPGIEDVAVLKKELVQVQTLMDKTTLDAENESEKLKDECRQLQAQISNSEVTITQLKAELAKGPQEVAVYVQELQQLKTALTELNQKHDSLMEKFLKKELDYTQLEEKQNEESTARKNLQVLLNQKDVEFQQLQARFSARDMSLQTVKAEVGEKAETNQKLKAELSEVETKYQHLKAEFKQLQQQREEKEQLSLQQQSEINQLHSKLLETERQLGEAHGRLKEQRQLSAEKLMDKEQQVADLQLKLSRAEEQLKEKTSASTDLQHQLEKIRQQHQEQTNLQQNTTSKLREAQNDLEQVLRQIGDKDQKIQNLEALLEKSKKNISLLEKEREDLYAKIQAGEGETAVLNQLQEKNHALQEQLTQLTEKLKNQSESHKQAQENLHEQVQEQKSQLRASQDRVLALEATLNEVTSQLNESKEKMVQLDLQIKAKTELLLAAEAAKTAQRVDLQNHLDTAQNALQDKQQELNKVNAQLEQVTAKLNDKQEYSIQLEANLKEYKEKHLNLEQKSEALEGQLKKLETDLISVKANREQTTQELQQQKQQSAQMELKSAELSKQLEEEKGLVSSMKLELQKKVESLQQSKEFISKQDQERNSLKENIEKLKQDLEKISGKMQDAVTSLEKTKLERDALQKDLAETKKKLSESSKSLQESRDQLEKEKLKGKKTVENMEKSQEEVKLNLQRQVDATAKEVNELKSMLENKQEESTQTKAELDKARGYVAKLHSAIKEKEKAEQELQTELKKMHETSDQEKVQHKSEIEAMTTQLAQKAEQEGKLQQQVKGLTSELETEKGKVSDLQKSHKLLQEKVNSLQSDMYGKESEVSAIRQDLKNTEEKLILAQEELKTNRNEVNGHKKLIQELTAVRGTLEQDIAKNKNLLKEREKTLQELQEQKVLIEKDISDEKLKVTNLNSLNEKLKGELIQTREELKSIKDECQKKTSSFEDAKQLLIQQKLELQAKLDSSASVLQQEKNSQQALKEDIKKKQEDWKKHSTEMREKLSAEAKEKEEQKKKHEENESKLNMQITALNENLATLKKEWQSSQRRVGELEKQTDDLRGEIAVLEATVQNNQDDRRALLERCLKSEGEIEKLQAKIFELRKKVDESSYAMEELGRENQSLQIKHNQALSRKWTEDHEVQNCMSCGRGFSVTIRRHHCRQCGNIFCHECSSKNALTPSSKKAVRVCDTCFGDLQG

Target ORF information:

RefSeq Version	XM_012959889.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis early endosome antigen 1 (eea1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012959889.2

ORF Insert Sequence:

ATGAAATCCC ATGCAACAGC TGAAGAACTT TTTGTGCACT ATGAAGCATC TCACGAACCT 
GCCAATGACC CTGGTCAAGG AGGACAAACC AATCTGTCGC CAAAAAGAGA TGAAGTGCTC 
TTACTTCGTC AGGAAGTCAA AGATTTACAG ACCTCATTAA AGGAAGAAAA ATGGTTTTCA 
GAAGAGTTGA AGAAAGAGTT GGAAAATGTT CAAGGGCAAC TGAAGCAAAA CACAATTTCA 
GATGGGATGA CCACAATTGC AGCTACAGAG CTTCAGTCTT TAGAACAGAA GTTAGAAGAG 
GCCCAGACAG AAATGTTTAA TATAAAGCAA ATGAAGGATT TATTTGAACA GAAAGCAGCT 
CAACAGGCTA CTGAAGTAGT TGATTTAAAG GCATCATATG ATGAAGAGAA GAGTAACTTG 
GCAGCTGCAC GGGAGAAAGT AAAAGGTTTA ACACAAGATC TGGATTCAAA GTTACACGTG 
ATTGAAGACT TGAAGAATGA GCTGCTTCAG CGACCTGGGA TTGAAGATGT TGCTGTGTTG 
AAGAAGGAGC TTGTCCAGGT CCAGACGCTG ATGGACAAAA CAACGCTAGA TGCTGAAAAT 
GAATCAGAGA AGCTGAAAGA TGAGTGCAGA CAGCTGCAGG CACAAATCTC TAACTCTGAG 
GTGACTATTA CTCAACTGAA AGCTGAACTT GCTAAAGGCC CTCAAGAAGT TGCAGTTTAT 
GTCCAGGAAT TGCAGCAACT CAAAACTGCT TTGACTGAGT TAAATCAAAA ACATGATAGT 
TTGATGGAAA AATTTTTGAA GAAGGAATTG GATTACACCC AGCTTGAGGA GAAACAAAAT 
GAGGAGTCCA CTGCAAGGAA AAACCTGCAA GTCCTTCTCA ATCAGAAGGA CGTGGAGTTC 
CAGCAATTGC AAGCTCGGTT CTCTGCAAGA GACATGTCGC TGCAGACCGT TAAGGCTGAG 
GTGGGCGAGA AGGCTGAGAC AAATCAGAAA CTAAAAGCGG AACTCTCAGA AGTCGAAACC 
AAATATCAGC ATCTCAAAGC CGAATTTAAG CAGCTTCAGC AGCAACGGGA AGAGAAGGAG 
CAGCTTAGTT TGCAGCAGCA AAGTGAGATC AATCAGCTTC ACAGCAAGTT ACTGGAGACA 
GAGCGCCAAC TAGGGGAAGC TCATGGGAGG CTGAAGGAGC AGAGGCAGCT GTCTGCTGAG 
AAACTGATGG ACAAGGAGCA GCAGGTGGCT GATCTACAGC TGAAACTCTC ACGTGCTGAG 
GAGCAGCTCA AAGAAAAGAC TTCCGCGTCT ACTGACTTGC AGCATCAATT GGAAAAGATA 
AGGCAACAGC ATCAGGAACA GACCAACCTC CAGCAGAATA CCACATCCAA ATTGAGAGAA 
GCCCAAAATG ATTTAGAACA AGTCTTACGG CAGATTGGGG ACAAAGACCA GAAAATCCAG 
AACCTTGAAG CACTATTGGA GAAAAGCAAA AAGAACATTT CTCTTCTTGA AAAAGAACGA 
GAAGATTTAT ATGCCAAAAT TCAAGCTGGT GAAGGAGAAA CTGCAGTTCT AAACCAACTT 
CAAGAGAAAA ACCACGCTTT GCAGGAACAG TTAACCCAGC TTACTGAGAA GTTAAAAAAT 
CAATCAGAAA GTCACAAACA AGCCCAGGAA AACTTGCATG AGCAGGTTCA GGAGCAGAAG 
TCCCAACTCA GAGCCTCCCA AGATCGTGTC CTGGCACTTG AGGCAACACT TAATGAAGTC 
ACCAGTCAGC TAAATGAGAG CAAAGAGAAA ATGGTTCAAC TGGATTTACA GATCAAAGCA 
AAAACTGAAC TTTTGTTAGC AGCAGAGGCA GCAAAGACAG CGCAAAGAGT AGATCTTCAG 
AACCACTTGG ATACAGCCCA AAATGCACTG CAGGATAAGC AACAGGAATT AAACAAAGTG 
AACGCACAAC TGGAACAAGT AACAGCAAAG TTAAATGACA AGCAGGAGTA CAGCATTCAA 
CTGGAGGCGA ACCTTAAGGA GTACAAAGAG AAGCACCTTA ATCTGGAACA AAAGTCTGAA 
GCTCTGGAAG GGCAGCTTAA GAAACTTGAA ACAGATCTAA TCAGTGTGAA AGCAAACCGG 
GAACAGACAA CCCAGGAGCT GCAGCAGCAG AAGCAGCAGA GCGCTCAGAT GGAATTGAAA 
TCTGCAGAGC TGAGCAAGCA GTTAGAAGAG GAGAAAGGAC TGGTATCAAG TATGAAACTG 
GAACTACAGA AGAAAGTGGA GTCTCTCCAG CAGTCAAAGG AATTCATTTC AAAACAAGAT 
CAAGAAAGGA ACAGTTTGAA GGAGAATATA GAAAAGTTAA AGCAAGACCT GGAGAAAATA 
TCAGGCAAAA TGCAAGATGC CGTGACAAGC CTAGAGAAAA CCAAACTGGA GAGAGACGCT 
TTGCAAAAAG ATCTTGCAGA GACCAAGAAG AAATTATCTG AGAGCAGCAA ATCTTTACAG 
GAATCAAGAG ATCAACTGGA AAAAGAAAAA CTGAAAGGAA AGAAAACAGT TGAAAACATG 
GAGAAGTCAC AGGAAGAAGT GAAATTGAAT CTACAAAGAC AGGTGGATGC AACTGCAAAG 
GAGGTGAATG AGCTCAAGTC TATGCTGGAA AATAAGCAGG AGGAATCCAC CCAGACAAAG 
GCTGAGTTGG ATAAAGCCCG TGGCTACGTT GCAAAGCTGC ACAGTGCTAT CAAAGAAAAG 
GAGAAAGCTG AGCAGGAGCT CCAGACAGAA CTGAAAAAGA TGCATGAAAC TTCAGATCAG 
GAGAAAGTGC AACACAAATC TGAGATTGAA GCCATGACCA CTCAGTTAGC ACAAAAGGCA 
GAACAAGAAG GAAAATTACA GCAGCAAGTG AAAGGATTAA CCAGTGAACT GGAAACTGAG 
AAAGGAAAAG TGTCTGATCT GCAGAAGTCC CACAAACTCC TTCAGGAGAA AGTGAATAGC 
CTTCAGTCAG ATATGTACGG GAAAGAGTCA GAAGTATCTG CAATACGGCA GGATCTGAAA 
AATACAGAGG AAAAGCTTAT TCTTGCACAG GAAGAGCTAA AAACAAACAG GAATGAAGTC 
AATGGCCACA AAAAGCTTAT ACAAGAACTA ACTGCTGTGA GAGGGACCTT GGAGCAGGAT 
ATAGCTAAAA ATAAGAACTT GCTGAAGGAA CGGGAAAAAA CACTGCAGGA ACTGCAGGAG 
CAGAAGGTCT TAATAGAGAA GGACATAAGT GATGAAAAAC TGAAAGTAAC TAATCTAAAC 
TCCTTAAATG AGAAGCTTAA GGGAGAATTA ATCCAGACTA GAGAAGAACT AAAGAGCATC 
AAAGATGAAT GCCAAAAGAA AACCAGCAGT TTTGAGGATG CCAAACAGCT ACTAATCCAG 
CAGAAGCTGG AGTTGCAGGC CAAACTTGAC AGCTCAGCTT CAGTTCTACA ACAGGAAAAG 
AATAGCCAAC AGGCTCTGAA GGAGGACATC AAGAAAAAGC AAGAGGACTG GAAAAAACAT 
AGCACGGAGA TGAGAGAGAA ATTGTCTGCA GAAGCAAAAG AAAAAGAAGA ACAAAAGAAG 
AAACATGAGG AGAATGAGTC AAAGCTAAAC ATGCAGATTA CAGCTTTAAA TGAGAACCTG 
GCAACGTTAA AGAAGGAATG GCAGAGCAGC CAACGTAGGG TCGGTGAGCT GGAGAAGCAA 
ACAGACGACC TGCGGGGAGA GATTGCAGTA TTAGAAGCAA CAGTCCAAAA CAATCAGGAC 
GACAGACGTG CTTTGTTAGA AAGGTGTCTT AAAAGCGAGG GTGAAATTGA AAAGCTCCAA 
GCCAAAATTT TCGAACTGCG GAAAAAAGTA GATGAATCTT CTTATGCAAT GGAGGAACTT 
GGCAGGGAAA ACCAATCACT ACAGATAAAG CACAACCAAG CACTCAGTAG GAAGTGGACA 
GAGGACCACG AAGTGCAGAA CTGCATGTCA TGTGGCAGAG GATTTTCTGT AACAATAAGA 
AGGCACCACT GTAGACAGTG TGGAAATATC TTCTGTCATG AATGTTCCTC GAAGAATGCC 
TTAACGCCCT CCTCCAAAAA AGCTGTGCGT GTTTGTGATA CATGTTTTGG TGACCTGCAA 
GGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eea1 ( XM_018092239.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017947728.1 Xenopus tropicalis early endosome antigen 1 (eea1), transcript variant X3, mRNA.

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CloneID	OXa32538
Clone ID Related Accession (Same CDS sequence)	XM_012959889.2 , XM_018092239.1
Accession Version	XM_018092239.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4086bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	early endosome antigen 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCC ATGCAACAGC TGAAGAACTT TTTGTGCACT ATGAAGCATC TCACGAACCT 
GCCAATGACC CTGGTCAAGG AGGACAAACC AATCTGTCGC CAAAAAGAGA TGAAGTGCTC 
TTACTTCGTC AGGAAGTCAA AGATTTACAG ACCTCATTAA AGGAAGAAAA ATGGTTTTCA 
GAAGAGTTGA AGAAAGAGTT GGAAAATGTT CAAGGGCAAC TGAAGCAAAA CACAATTTCA 
GATGGGATGA CCACAATTGC AGCTACAGAG CTTCAGTCTT TAGAACAGAA GTTAGAAGAG 
GCCCAGACAG AAATGTTTAA TATAAAGCAA ATGAAGGATT TATTTGAACA GAAAGCAGCT 
CAACAGGCTA CTGAAGTAGT TGATTTAAAG GCATCATATG ATGAAGAGAA GAGTAACTTG 
GCAGCTGCAC GGGAGAAAGT AAAAGGTTTA ACACAAGATC TGGATTCAAA GTTACACGTG 
ATTGAAGACT TGAAGAATGA GCTGCTTCAG CGACCTGGGA TTGAAGATGT TGCTGTGTTG 
AAGAAGGAGC TTGTCCAGGT CCAGACGCTG ATGGACAAAA CAACGCTAGA TGCTGAAAAT 
GAATCAGAGA AGCTGAAAGA TGAGTGCAGA CAGCTGCAGG CACAAATCTC TAACTCTGAG 
GTGACTATTA CTCAACTGAA AGCTGAACTT GCTAAAGGCC CTCAAGAAGT TGCAGTTTAT 
GTCCAGGAAT TGCAGCAACT CAAAACTGCT TTGACTGAGT TAAATCAAAA ACATGATAGT 
TTGATGGAAA AATTTTTGAA GAAGGAATTG GATTACACCC AGCTTGAGGA GAAACAAAAT 
GAGGAGTCCA CTGCAAGGAA AAACCTGCAA GTCCTTCTCA ATCAGAAGGA CGTGGAGTTC 
CAGCAATTGC AAGCTCGGTT CTCTGCAAGA GACATGTCGC TGCAGACCGT TAAGGCTGAG 
GTGGGCGAGA AGGCTGAGAC AAATCAGAAA CTAAAAGCGG AACTCTCAGA AGTCGAAACC 
AAATATCAGC ATCTCAAAGC CGAATTTAAG CAGCTTCAGC AGCAACGGGA AGAGAAGGAG 
CAGCTTAGTT TGCAGCAGCA AAGTGAGATC AATCAGCTTC ACAGCAAGTT ACTGGAGACA 
GAGCGCCAAC TAGGGGAAGC TCATGGGAGG CTGAAGGAGC AGAGGCAGCT GTCTGCTGAG 
AAACTGATGG ACAAGGAGCA GCAGGTGGCT GATCTACAGC TGAAACTCTC ACGTGCTGAG 
GAGCAGCTCA AAGAAAAGAC TTCCGCGTCT ACTGACTTGC AGCATCAATT GGAAAAGATA 
AGGCAACAGC ATCAGGAACA GACCAACCTC CAGCAGAATA CCACATCCAA ATTGAGAGAA 
GCCCAAAATG ATTTAGAACA AGTCTTACGG CAGATTGGGG ACAAAGACCA GAAAATCCAG 
AACCTTGAAG CACTATTGGA GAAAAGCAAA AAGAACATTT CTCTTCTTGA AAAAGAACGA 
GAAGATTTAT ATGCCAAAAT TCAAGCTGGT GAAGGAGAAA CTGCAGTTCT AAACCAACTT 
CAAGAGAAAA ACCACGCTTT GCAGGAACAG TTAACCCAGC TTACTGAGAA GTTAAAAAAT 
CAATCAGAAA GTCACAAACA AGCCCAGGAA AACTTGCATG AGCAGGTTCA GGAGCAGAAG 
TCCCAACTCA GAGCCTCCCA AGATCGTGTC CTGGCACTTG AGGCAACACT TAATGAAGTC 
ACCAGTCAGC TAAATGAGAG CAAAGAGAAA ATGGTTCAAC TGGATTTACA GATCAAAGCA 
AAAACTGAAC TTTTGTTAGC AGCAGAGGCA GCAAAGACAG CGCAAAGAGT AGATCTTCAG 
AACCACTTGG ATACAGCCCA AAATGCACTG CAGGATAAGC AACAGGAATT AAACAAAGTG 
AACGCACAAC TGGAACAAGT AACAGCAAAG TTAAATGACA AGCAGGAGTA CAGCATTCAA 
CTGGAGGCGA ACCTTAAGGA GTACAAAGAG AAGCACCTTA ATCTGGAACA AAAGTCTGAA 
GCTCTGGAAG GGCAGCTTAA GAAACTTGAA ACAGATCTAA TCAGTGTGAA AGCAAACCGG 
GAACAGACAA CCCAGGAGCT GCAGCAGCAG AAGCAGCAGA GCGCTCAGAT GGAATTGAAA 
TCTGCAGAGC TGAGCAAGCA GTTAGAAGAG GAGAAAGGAC TGGTATCAAG TATGAAACTG 
GAACTACAGA AGAAAGTGGA GTCTCTCCAG CAGTCAAAGG AATTCATTTC AAAACAAGAT 
CAAGAAAGGA ACAGTTTGAA GGAGAATATA GAAAAGTTAA AGCAAGACCT GGAGAAAATA 
TCAGGCAAAA TGCAAGATGC CGTGACAAGC CTAGAGAAAA CCAAACTGGA GAGAGACGCT 
TTGCAAAAAG ATCTTGCAGA GACCAAGAAG AAATTATCTG AGAGCAGCAA ATCTTTACAG 
GAATCAAGAG ATCAACTGGA AAAAGAAAAA CTGAAAGGAA AGAAAACAGT TGAAAACATG 
GAGAAGTCAC AGGAAGAAGT GAAATTGAAT CTACAAAGAC AGGTGGATGC AACTGCAAAG 
GAGGTGAATG AGCTCAAGTC TATGCTGGAA AATAAGCAGG AGGAATCCAC CCAGACAAAG 
GCTGAGTTGG ATAAAGCCCG TGGCTACGTT GCAAAGCTGC ACAGTGCTAT CAAAGAAAAG 
GAGAAAGCTG AGCAGGAGCT CCAGACAGAA CTGAAAAAGA TGCATGAAAC TTCAGATCAG 
GAGAAAGTGC AACACAAATC TGAGATTGAA GCCATGACCA CTCAGTTAGC ACAAAAGGCA 
GAACAAGAAG GAAAATTACA GCAGCAAGTG AAAGGATTAA CCAGTGAACT GGAAACTGAG 
AAAGGAAAAG TGTCTGATCT GCAGAAGTCC CACAAACTCC TTCAGGAGAA AGTGAATAGC 
CTTCAGTCAG ATATGTACGG GAAAGAGTCA GAAGTATCTG CAATACGGCA GGATCTGAAA 
AATACAGAGG AAAAGCTTAT TCTTGCACAG GAAGAGCTAA AAACAAACAG GAATGAAGTC 
AATGGCCACA AAAAGCTTAT ACAAGAACTA ACTGCTGTGA GAGGGACCTT GGAGCAGGAT 
ATAGCTAAAA ATAAGAACTT GCTGAAGGAA CGGGAAAAAA CACTGCAGGA ACTGCAGGAG 
CAGAAGGTCT TAATAGAGAA GGACATAAGT GATGAAAAAC TGAAAGTAAC TAATCTAAAC 
TCCTTAAATG AGAAGCTTAA GGGAGAATTA ATCCAGACTA GAGAAGAACT AAAGAGCATC 
AAAGATGAAT GCCAAAAGAA AACCAGCAGT TTTGAGGATG CCAAACAGCT ACTAATCCAG 
CAGAAGCTGG AGTTGCAGGC CAAACTTGAC AGCTCAGCTT CAGTTCTACA ACAGGAAAAG 
AATAGCCAAC AGGCTCTGAA GGAGGACATC AAGAAAAAGC AAGAGGACTG GAAAAAACAT 
AGCACGGAGA TGAGAGAGAA ATTGTCTGCA GAAGCAAAAG AAAAAGAAGA ACAAAAGAAG 
AAACATGAGG AGAATGAGTC AAAGCTAAAC ATGCAGATTA CAGCTTTAAA TGAGAACCTG 
GCAACGTTAA AGAAGGAATG GCAGAGCAGC CAACGTAGGG TCGGTGAGCT GGAGAAGCAA 
ACAGACGACC TGCGGGGAGA GATTGCAGTA TTAGAAGCAA CAGTCCAAAA CAATCAGGAC 
GACAGACGTG CTTTGTTAGA AAGGTGTCTT AAAAGCGAGG GTGAAATTGA AAAGCTCCAA 
GCCAAAATTT TCGAACTGCG GAAAAAAGTA GATGAATCTT CTTATGCAAT GGAGGAACTT 
GGCAGGGAAA ACCAATCACT ACAGATAAAG CACAACCAAG CACTCAGTAG GAAGTGGACA 
GAGGACCACG AAGTGCAGAA CTGCATGTCA TGTGGCAGAG GATTTTCTGT AACAATAAGA 
AGGCACCACT GTAGACAGTG TGGAAATATC TTCTGTCATG AATGTTCCTC GAAGAATGCC 
TTAACGCCCT CCTCCAAAAA AGCTGTGCGT GTTTGTGATA CATGTTTTGG TGACCTGCAA 
GGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017947728.1
CDS	1029..5114
Translation	MKSHATAEELFVHYEASHEPANDPGQGGQTNLSPKRDEVLLLRQEVKDLQTSLKEEKWFSEELKKELENVQGQLKQNTISDGMTTIAATELQSLEQKLEEAQTEMFNIKQMKDLFEQKAAQQATEVVDLKASYDEEKSNLAAAREKVKGLTQDLDSKLHVIEDLKNELLQRPGIEDVAVLKKELVQVQTLMDKTTLDAENESEKLKDECRQLQAQISNSEVTITQLKAELAKGPQEVAVYVQELQQLKTALTELNQKHDSLMEKFLKKELDYTQLEEKQNEESTARKNLQVLLNQKDVEFQQLQARFSARDMSLQTVKAEVGEKAETNQKLKAELSEVETKYQHLKAEFKQLQQQREEKEQLSLQQQSEINQLHSKLLETERQLGEAHGRLKEQRQLSAEKLMDKEQQVADLQLKLSRAEEQLKEKTSASTDLQHQLEKIRQQHQEQTNLQQNTTSKLREAQNDLEQVLRQIGDKDQKIQNLEALLEKSKKNISLLEKEREDLYAKIQAGEGETAVLNQLQEKNHALQEQLTQLTEKLKNQSESHKQAQENLHEQVQEQKSQLRASQDRVLALEATLNEVTSQLNESKEKMVQLDLQIKAKTELLLAAEAAKTAQRVDLQNHLDTAQNALQDKQQELNKVNAQLEQVTAKLNDKQEYSIQLEANLKEYKEKHLNLEQKSEALEGQLKKLETDLISVKANREQTTQELQQQKQQSAQMELKSAELSKQLEEEKGLVSSMKLELQKKVESLQQSKEFISKQDQERNSLKENIEKLKQDLEKISGKMQDAVTSLEKTKLERDALQKDLAETKKKLSESSKSLQESRDQLEKEKLKGKKTVENMEKSQEEVKLNLQRQVDATAKEVNELKSMLENKQEESTQTKAELDKARGYVAKLHSAIKEKEKAEQELQTELKKMHETSDQEKVQHKSEIEAMTTQLAQKAEQEGKLQQQVKGLTSELETEKGKVSDLQKSHKLLQEKVNSLQSDMYGKESEVSAIRQDLKNTEEKLILAQEELKTNRNEVNGHKKLIQELTAVRGTLEQDIAKNKNLLKEREKTLQELQEQKVLIEKDISDEKLKVTNLNSLNEKLKGELIQTREELKSIKDECQKKTSSFEDAKQLLIQQKLELQAKLDSSASVLQQEKNSQQALKEDIKKKQEDWKKHSTEMREKLSAEAKEKEEQKKKHEENESKLNMQITALNENLATLKKEWQSSQRRVGELEKQTDDLRGEIAVLEATVQNNQDDRRALLERCLKSEGEIEKLQAKIFELRKKVDESSYAMEELGRENQSLQIKHNQALSRKWTEDHEVQNCMSCGRGFSVTIRRHHCRQCGNIFCHECSSKNALTPSSKKAVRVCDTCFGDLQG

Target ORF information:

RefSeq Version	XM_018092239.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis early endosome antigen 1 (eea1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018092239.1

ORF Insert Sequence:

ATGAAATCCC ATGCAACAGC TGAAGAACTT TTTGTGCACT ATGAAGCATC TCACGAACCT 
GCCAATGACC CTGGTCAAGG AGGACAAACC AATCTGTCGC CAAAAAGAGA TGAAGTGCTC 
TTACTTCGTC AGGAAGTCAA AGATTTACAG ACCTCATTAA AGGAAGAAAA ATGGTTTTCA 
GAAGAGTTGA AGAAAGAGTT GGAAAATGTT CAAGGGCAAC TGAAGCAAAA CACAATTTCA 
GATGGGATGA CCACAATTGC AGCTACAGAG CTTCAGTCTT TAGAACAGAA GTTAGAAGAG 
GCCCAGACAG AAATGTTTAA TATAAAGCAA ATGAAGGATT TATTTGAACA GAAAGCAGCT 
CAACAGGCTA CTGAAGTAGT TGATTTAAAG GCATCATATG ATGAAGAGAA GAGTAACTTG 
GCAGCTGCAC GGGAGAAAGT AAAAGGTTTA ACACAAGATC TGGATTCAAA GTTACACGTG 
ATTGAAGACT TGAAGAATGA GCTGCTTCAG CGACCTGGGA TTGAAGATGT TGCTGTGTTG 
AAGAAGGAGC TTGTCCAGGT CCAGACGCTG ATGGACAAAA CAACGCTAGA TGCTGAAAAT 
GAATCAGAGA AGCTGAAAGA TGAGTGCAGA CAGCTGCAGG CACAAATCTC TAACTCTGAG 
GTGACTATTA CTCAACTGAA AGCTGAACTT GCTAAAGGCC CTCAAGAAGT TGCAGTTTAT 
GTCCAGGAAT TGCAGCAACT CAAAACTGCT TTGACTGAGT TAAATCAAAA ACATGATAGT 
TTGATGGAAA AATTTTTGAA GAAGGAATTG GATTACACCC AGCTTGAGGA GAAACAAAAT 
GAGGAGTCCA CTGCAAGGAA AAACCTGCAA GTCCTTCTCA ATCAGAAGGA CGTGGAGTTC 
CAGCAATTGC AAGCTCGGTT CTCTGCAAGA GACATGTCGC TGCAGACCGT TAAGGCTGAG 
GTGGGCGAGA AGGCTGAGAC AAATCAGAAA CTAAAAGCGG AACTCTCAGA AGTCGAAACC 
AAATATCAGC ATCTCAAAGC CGAATTTAAG CAGCTTCAGC AGCAACGGGA AGAGAAGGAG 
CAGCTTAGTT TGCAGCAGCA AAGTGAGATC AATCAGCTTC ACAGCAAGTT ACTGGAGACA 
GAGCGCCAAC TAGGGGAAGC TCATGGGAGG CTGAAGGAGC AGAGGCAGCT GTCTGCTGAG 
AAACTGATGG ACAAGGAGCA GCAGGTGGCT GATCTACAGC TGAAACTCTC ACGTGCTGAG 
GAGCAGCTCA AAGAAAAGAC TTCCGCGTCT ACTGACTTGC AGCATCAATT GGAAAAGATA 
AGGCAACAGC ATCAGGAACA GACCAACCTC CAGCAGAATA CCACATCCAA ATTGAGAGAA 
GCCCAAAATG ATTTAGAACA AGTCTTACGG CAGATTGGGG ACAAAGACCA GAAAATCCAG 
AACCTTGAAG CACTATTGGA GAAAAGCAAA AAGAACATTT CTCTTCTTGA AAAAGAACGA 
GAAGATTTAT ATGCCAAAAT TCAAGCTGGT GAAGGAGAAA CTGCAGTTCT AAACCAACTT 
CAAGAGAAAA ACCACGCTTT GCAGGAACAG TTAACCCAGC TTACTGAGAA GTTAAAAAAT 
CAATCAGAAA GTCACAAACA AGCCCAGGAA AACTTGCATG AGCAGGTTCA GGAGCAGAAG 
TCCCAACTCA GAGCCTCCCA AGATCGTGTC CTGGCACTTG AGGCAACACT TAATGAAGTC 
ACCAGTCAGC TAAATGAGAG CAAAGAGAAA ATGGTTCAAC TGGATTTACA GATCAAAGCA 
AAAACTGAAC TTTTGTTAGC AGCAGAGGCA GCAAAGACAG CGCAAAGAGT AGATCTTCAG 
AACCACTTGG ATACAGCCCA AAATGCACTG CAGGATAAGC AACAGGAATT AAACAAAGTG 
AACGCACAAC TGGAACAAGT AACAGCAAAG TTAAATGACA AGCAGGAGTA CAGCATTCAA 
CTGGAGGCGA ACCTTAAGGA GTACAAAGAG AAGCACCTTA ATCTGGAACA AAAGTCTGAA 
GCTCTGGAAG GGCAGCTTAA GAAACTTGAA ACAGATCTAA TCAGTGTGAA AGCAAACCGG 
GAACAGACAA CCCAGGAGCT GCAGCAGCAG AAGCAGCAGA GCGCTCAGAT GGAATTGAAA 
TCTGCAGAGC TGAGCAAGCA GTTAGAAGAG GAGAAAGGAC TGGTATCAAG TATGAAACTG 
GAACTACAGA AGAAAGTGGA GTCTCTCCAG CAGTCAAAGG AATTCATTTC AAAACAAGAT 
CAAGAAAGGA ACAGTTTGAA GGAGAATATA GAAAAGTTAA AGCAAGACCT GGAGAAAATA 
TCAGGCAAAA TGCAAGATGC CGTGACAAGC CTAGAGAAAA CCAAACTGGA GAGAGACGCT 
TTGCAAAAAG ATCTTGCAGA GACCAAGAAG AAATTATCTG AGAGCAGCAA ATCTTTACAG 
GAATCAAGAG ATCAACTGGA AAAAGAAAAA CTGAAAGGAA AGAAAACAGT TGAAAACATG 
GAGAAGTCAC AGGAAGAAGT GAAATTGAAT CTACAAAGAC AGGTGGATGC AACTGCAAAG 
GAGGTGAATG AGCTCAAGTC TATGCTGGAA AATAAGCAGG AGGAATCCAC CCAGACAAAG 
GCTGAGTTGG ATAAAGCCCG TGGCTACGTT GCAAAGCTGC ACAGTGCTAT CAAAGAAAAG 
GAGAAAGCTG AGCAGGAGCT CCAGACAGAA CTGAAAAAGA TGCATGAAAC TTCAGATCAG 
GAGAAAGTGC AACACAAATC TGAGATTGAA GCCATGACCA CTCAGTTAGC ACAAAAGGCA 
GAACAAGAAG GAAAATTACA GCAGCAAGTG AAAGGATTAA CCAGTGAACT GGAAACTGAG 
AAAGGAAAAG TGTCTGATCT GCAGAAGTCC CACAAACTCC TTCAGGAGAA AGTGAATAGC 
CTTCAGTCAG ATATGTACGG GAAAGAGTCA GAAGTATCTG CAATACGGCA GGATCTGAAA 
AATACAGAGG AAAAGCTTAT TCTTGCACAG GAAGAGCTAA AAACAAACAG GAATGAAGTC 
AATGGCCACA AAAAGCTTAT ACAAGAACTA ACTGCTGTGA GAGGGACCTT GGAGCAGGAT 
ATAGCTAAAA ATAAGAACTT GCTGAAGGAA CGGGAAAAAA CACTGCAGGA ACTGCAGGAG 
CAGAAGGTCT TAATAGAGAA GGACATAAGT GATGAAAAAC TGAAAGTAAC TAATCTAAAC 
TCCTTAAATG AGAAGCTTAA GGGAGAATTA ATCCAGACTA GAGAAGAACT AAAGAGCATC 
AAAGATGAAT GCCAAAAGAA AACCAGCAGT TTTGAGGATG CCAAACAGCT ACTAATCCAG 
CAGAAGCTGG AGTTGCAGGC CAAACTTGAC AGCTCAGCTT CAGTTCTACA ACAGGAAAAG 
AATAGCCAAC AGGCTCTGAA GGAGGACATC AAGAAAAAGC AAGAGGACTG GAAAAAACAT 
AGCACGGAGA TGAGAGAGAA ATTGTCTGCA GAAGCAAAAG AAAAAGAAGA ACAAAAGAAG 
AAACATGAGG AGAATGAGTC AAAGCTAAAC ATGCAGATTA CAGCTTTAAA TGAGAACCTG 
GCAACGTTAA AGAAGGAATG GCAGAGCAGC CAACGTAGGG TCGGTGAGCT GGAGAAGCAA 
ACAGACGACC TGCGGGGAGA GATTGCAGTA TTAGAAGCAA CAGTCCAAAA CAATCAGGAC 
GACAGACGTG CTTTGTTAGA AAGGTGTCTT AAAAGCGAGG GTGAAATTGA AAAGCTCCAA 
GCCAAAATTT TCGAACTGCG GAAAAAAGTA GATGAATCTT CTTATGCAAT GGAGGAACTT 
GGCAGGGAAA ACCAATCACT ACAGATAAAG CACAACCAAG CACTCAGTAG GAAGTGGACA 
GAGGACCACG AAGTGCAGAA CTGCATGTCA TGTGGCAGAG GATTTTCTGT AACAATAAGA 
AGGCACCACT GTAGACAGTG TGGAAATATC TTCTGTCATG AATGTTCCTC GAAGAATGCC 
TTAACGCCCT CCTCCAAAAA AGCTGTGCGT GTTTGTGATA CATGTTTTGG TGACCTGCAA 
GGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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eea1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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