KNL1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	KNL1
Entrez Gene ID	57082
Full Name	kinetochore scaffold 1
Synonyms	AF15Q14,CASC5,CT29,D40,MCPH4,PPP1R55,Spc7,hKNL-1,hSpc105
General protein information	Preferred Names kinetochore scaffold 1 Names kinetochore scaffold 1 protein CASC5 ALL1-fused gene from chromosome 15q14 protein blinkin, bub-linking kinetochore protein cancer susceptibility candidate 5 cancer susceptibility candidate gene 5 protein cancer/testis antigen 29 kinetochore null 1 homolog kinetochore-null protein 1 microcephaly, primary autosomal recessive 4 protein phosphatase 1, regulatory subunit 55
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 15 Map Location: 15q15.1
Summary	The protein encoded by this gene is a component of the multiprotein assembly that is required for creation of kinetochore-microtubule attachments and chromosome segregation. The encoded protein functions as a scaffold for proteins that influence the spindle assembly checkpoint during the eukaryotic cell cycle and it interacts with at least five different kinetochore proteins and two checkpoint kinases. In adults, this gene is predominantly expressed in normal testes, various cancer cell lines and primary tumors from other tissues and is ubiquitously expressed in fetal tissues. This gene was originally identified as a fusion partner with the mixed-lineage leukemia (MLL) gene in t(11;15)(q23;q14). Mutations in this gene cause autosomal recessive primary microcephaly-4 (MCPH4). Alternative splicing results in multiple transcript variants encoding different isoforms. Additional splice variants have been described but their biological validity has not been confirmed. [provided by RefSeq, Jan 2013].
Disorder MIM:	609173
Disorder Html:	Microcephaly 4, primary, autosomal recessive, 604321 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_144508.4	NP_653091.3	kinetochore scaffold 1 isoform 2
NM_170589.5	NP_733468.3	kinetochore scaffold 1 isoform 1
NM_170589.4	NP_733468.3	kinetochore scaffold 1 isoform 1
NM_144508.5	NP_653091.3	kinetochore scaffold 1 isoform 2
XM_017022432.1	XP_016877921.1	kinetochore scaffold 1 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	CASC5	NP_733468.3
Pan troglodytes (chimpanzee)	CASC5	XP_510312.3
Canis lupus familiaris (dog)	CASC5	XP_005638312.1
Bos taurus (cattle)	CASC5	XP_002690867.3
Mus musculus (house mouse)	Casc5	NP_083893.2
Rattus norvegicus (Norway rat)	Casc5	NP_001164065.1
Gallus gallus (chicken)	CASC5	XP_420938.4

Gene Ontology
Bibliography

Related articles in PubMed

D40/KNL1/CASC5 and autosomal recessive primary microcephaly.
Takimoto M
Congenital anomalies57(6)191-196(2017 Nov)

A sequential multi-target Mps1 phosphorylation cascade promotes spindle checkpoint signaling.
Ji Z, Gao H, Jia L, Li B, Yu H
eLife6(2017 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CASC5 may contribute to the morphological and structural changes of the human brain during recent evolution. The observed between-population divergence of CASC5 variants was driven by natural selection that tends to favor a larger gray matter volume in East Asians.
Title: Regional selection of the brain size regulating gene CASC5 provides new insight into human brain evolution.

We propose that CASC5 has a key role for the correct functioning of DNA damage response proteins, and a defect in this connection might affect upstream and downstream DNA damage response events as response to increased genotoxic stress.
Title: A novel homozygous splicing mutation of CASC5 causes primary microcephaly in a large Pakistani family.

Involvement of CASC5 in autosomal recessive microcephaly and a founder effect of the c.6125G>A mutation.
Title: Refining the phenotype associated with CASC5 mutation.

Targeted Knockdown of the Kinetochore Protein D40/Knl-1 Inhibits Human Cancer in a p53 Status-Independent Manner
Title: Targeted Knockdown of the Kinetochore Protein D40/Knl-1 Inhibits Human Cancer in a p53 Status-Independent Manner.

Data show sequential multisite regulation of the microtubule-associated protein KNL1-kinase-adaptor complex BUB1/BUB3 interaction.
Title: Sequential multisite phospho-regulation of KNL1-BUB3 interfaces at mitotic kinetochores.

The following KNL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KNL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu20484	NM_170589.5 Latest version!	Homo sapiens kinetochore scaffold 1 (KNL1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$923.30 $1319.00
OHu21655	NM_144508.5 Latest version!	Homo sapiens kinetochore scaffold 1 (KNL1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$923.30 $1319.00
OHu70263	XM_017022432.1 Latest version!	Homo sapiens kinetochore scaffold 1 (KNL1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
View Old Accession Versions >>
OHu20484	NM_170589.4	Homo sapiens kinetochore scaffold 1 (KNL1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$923.30 $1319.00
OHu21655	NM_144508.4	Homo sapiens kinetochore scaffold 1 (KNL1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$923.30 $1319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

KNL1 ( NM_170589.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_733468.3 Homo sapiens kinetochore scaffold 1 (KNL1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu20484
Clone ID Related Accession (Same CDS sequence)	NM_170589.5 , NM_170589.4
Accession Version	NM_170589.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7029bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	kinetochore scaffold 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC014306.1, AF461041.1, AF173994.1, AC022405.5 and AB046790.1. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_170589.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF173994.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968968, SAMEA2142586 [ECO:0000350] ##Evidence-Data-END##

ATGGATGGGG TGTCTTCAGA GGCTAATGAA GAAAATGACA ATATAGAGAG ACCTGTTAGA 
AGACGGCATT CTTCAATATT GAAACCCCCA AGGAGTCCTC TTCAGGACCT CAGAGGTGGG 
AATGAAAGAG TTCAGGAATC CAATGCTTTG AGAAATAAGA AAAACTCTCG TCGAGTCAGC 
TTTGCAGATA CTATAAAGGT ATTCCAGACG GAGTCTCATA TGAAAATAGT GAGAAAGTCA 
GAAATGGAAG GCTGCAGTGC AATGGTGCCA TCTCAGCTGC AACTTCTGCC TCCTGGGTTT 
AAGCGATTCT CCTGCCTCAG CCTCCCAGAA ACAGAAACAG GAGAAAATCT TCTTTTGATA 
CAGAATAAGA AATTAGAAGA TAATTACTGT GAAATTACTG GGATGAACAC ATTGCTTTCT 
GCTCCCATTC ATACCCAGAT GCAACAGAAG GAGTTTTCAA TTATAGAACA TACCCGTGAA 
AGGAAACATG CAAATGACCA GACAGTCATT TTTTCAGATG AAAACCAGAT GGACCTGACA 
TCAAGTCACA CTGTAATGAT TACCAAAGGC CTTTTAGATA ATCCCATAAG TGAAAAGTCC 
ACCAAGATAG ATACCACATC ATTTCTAGCT AATTTAAAGC TTCACACCGA GGACTCAAGA 
ATGAAAAAAG AAGTAAATTT TTCCGTGGAT CAAAACACTT CTTCAGAAAA TAAAATAGAT 
TTCAATGACT TCATAAAAAG ATTGAAAACA GGAAAATGTA GTGCTTTTCC TGATGTGCCT 
GATAAAGAAA ATTTTGAGAT ACCTATTTAT TCCAAGGAAC CGAACAGTGC CTCTTCTACA 
CATCAAATGC ATGTATCTCT TAAGGAAGAT GAAAATAACA GTAATATTAC TAGGCTCTTT 
AGAGAAAAAG ATGATGGGAT GAATTTCACC CAGTGTCATA CAGCCAATAT TCAGACATTG 
ATTCCCACAT CCAGTGAGAC CAACTCACGG GAATCTAAAG GTAATGATAT TACAATTTAT 
GGCAATGACT TTATGGACTT GACATTTAAC CACACTTTGC AGATCTTACC TGCAACAGGT 
AATTTTTCTG AAATAGAAAA TCAAACTCAG AATGCCATGG ATGTAACAAC AGGTTATGGA 
ACTAAAGCTT CAGGAAATAA AACAGTTTTT AAGAGTAAAC AAAATACTGC TTTTCAAGAC 
CTTTCCATAA ACTCTGCAGA CAAAATACAT ATTACCAGAA GTCATATTAT GGGGGCAGAA 
ACTCACATAG TCTCACAGAC TTGTAATCAG GATGCCAGAA TATTAGCCAT GACCCCAGAA 
TCTATATATT CTAATCCATC TATTCAAGGT TGTAAGACTG TTTTCTATTC TAGTTGTAAT 
GATGCCATGG AAATGACCAA ATGTCTCTCA AATATGAGAG AGGAGAAAAA TTTGCTAAAG 
CATGACAGTA ATTATGCTAA AATGTATTGC AATCCAGATG CTATGTCTTC TCTCACAGAG 
AAAACTATTT ATTCCGGAGA GGAGAACATG GACATTACCA AGAGTCATAC AGTTGCAATA 
GATAATCAAA TTTTTAAACA AGATCAATCA AATGTGCAAA TAGCAGCTGC ACCAACACCC 
GAAAAAGAAA TGATGCTCCA AAATCTTATG ACCACATCAG AAGATGGGAA AATGAATGTA 
AATTGTAACT CAGTTCCTCA TGTATCTAAG GAAAGAATAC AGCAGAGCCT GTCAAATCCT 
TTGTCTATTT CATTGACTGA TAGAAAGACT GAACTCTTAT CAGGTGAAAA TATGGATTTG 
ACTGAAAGTC ACACAAGTAA CTTAGGAAGT CAGGTTCCTC TTGCAGCTTA TAATCTAGCA 
CCGGAGAGTA CCAGTGAATC TCACTCTCAG AGCAAAAGCT CTTCAGATGA ATGTGAAGAA 
ATTACCAAAA GTCGTAATGA ACCATTTCAG CGATCAGACA TAATAGCCAA AAACAGCTTA 
ACCGACACCT GGAACAAAGA CAAAGATTGG GTTTTGAAGA TTTTGCCCTA CCTTGATAAA 
GATTCTCCTC AGTCAGCTGA TTGTAATCAG GAGATAGCAA CAAGCCATAA TATAGTCTAC 
TGTGGTGGAG TTCTTGATAA ACAAATAACT AATAGAAATA CAGTATCATG GGAACAATCT 
TTGTTTTCTA CCACAAAGCC ATTATTTTCA TCAGGACAGT TCTCTATGAA AAATCATGAT 
ACTGCTATAA GTAGTCATAC AGTGAAATCT GTACTAGGCC AGAATTCTAA ACTGGCTGAG 
CCACTGAGGA AAAGTTTAAG CAATCCCACA CCTGACTATT GCCATGACAA GATGATTATA 
TGTTCAGAGG AAGAGCAAAA TATGGATCTA ACAAAGAGCC ACACTGTCGT CATTGGATTT 
GGTCCTTCTG AACTACAAGA ACTTGGTAAA ACTAATTTAG AACACACTAC TGGCCAGCTA 
ACAACAATGA ACAGACAGAT AGCTGTAAAA GTTGAAAAAT GTGGTAAAAG TCCCATAGAA 
AAAAGTGGAG TGCTTAAATC TAACTGTATT ATGGATGTGT TAGAGGACGA AAGTGTACAG 
AAACCTAAAT TTCCAAAGGA AAAGCAAAAT GTCAAAATTT GGGGAAGGAA AAGTGTTGGT 
GGACCAAAAA TTGATAAGAC TATTGTATTT TCAGAAGACG ATAAGAATGA TATGGATATC 
ACTAAGAGTT ATACAATAGA AATAAACCAT AGACCTTTAT TAGAGAAACG TGATTGTCAT 
TTGGTGCCAT TGGCAGGAAC TTCTGAAACT ATTTTATATA CATGTAGGCA GGATGACATG 
GAGATCACTA GAAGTCACAC AACTGCCTTA GAATGTAAAA CTGTCTCACC AGATGAAATA 
ACTACTAGGC CTATGGACAA AACTGTAGTG TTTGTAGATA ATCATGTTGA ACTAGAAATG 
ACAGAGTCCC ATACTGTTTT CATTGACTAC CAAGAAAAGG AAAGAACAGA CAGACCTAAC 
TTTGAACTAT CCCAAAGGAA AAGCCTAGGA ACACCAACAG TGATATGTAC TCCTACTGAG 
GAGAGTGTTT TCTTTCCAGG AAATGGTGAA AGTGACCGTC TAGTAGCAAA TGACAGCCAG 
CTAACCCCTC TGGAGGAATG GTCTAATAAT AGGGGCCCTG TAGAGGTAGC TGATAACATG 
GAATTGTCTA AATCAGCCAC TTGCAAAAAC ATCAAAGATG TACAAAGTCC TGGATTTCTG 
AATGAACCTC TATCAAGCAA AAGTCAGAGA AGAAAAAGCC TTAAGCTAAA AAATGACAAG 
ACCATTGTAT TTTCAGAGAA TCATAAAAAT GATATGGATA TTACCCAGAG TTGTATGGTG 
GAAATAGATA ACGAAAGTGC CCTGGAGGAT AAAGAGGACT TCCATTTGGC AGGGGCTTCT 
AAAACTATTT TGTATTCATG TGGGCAGGAT GACATGGAGA TCACTAGGAG TCACACAACT 
GCCTTAGAAT GTAAAACTCT CCTGCCAAAT GAAATAGCTA TTAGGCCCAT GGACAAAACC 
GTATTGTTCA CAGATAATTA CAGTGATCTG GAAGTCACCG ATTCCCATAC TGTTTTCATT 
GACTGTCAAG CCACAGAGAA AATACTTGAA GAAAACCCTA AATTTGGAAT AGGAAAAGGA 
AAAAACTTGG GTGTTTCCTT TCCTAAGGAT AATAGCTGTG TTCAAGAAAT CGCTGAAAAA 
CAAGCACTGG CTGTAGGAAA CAAAATAGTT CTTCACACCG AGCAAAAGCA ACAACTCTTT 
GCTGCTACTA ATAGAACTAC TAATGAAATC ATCAAATTTC ATAGTGCTGC TATGGATGAA 
AAGGTCATAG GGAAAGTTGT AGACCAGGCC TGTACATTGG AAAAAGCGCA AGTTGAAAGC 
TGTCAGTTAA ATAATAGAGA TAGAAGAAAT GTGGACTTTA CAAGTAGTCA TGCAACTGCT 
GTTTGTGGAT CCAGTGATAA TTATTCCTGT TTACCAAATG TTATTTCCTG TACTGATAAT 
TTGGAGGGTA GTGCCATGCT CTTATGTGAT AAAGATGAGG AAAAAGCCAA TTATTGCCCA 
GTGCAAAATG ATCTTGCTTA TGCAAATGAT TTTGCCAGTG AATATTACTT GGAATCTGAG 
GGACAGCCTC TCTCTGCTCC TTGTCCTTTG TTAGAGAAGG AAGAAGTTAT TCAAACCAGT 
ACCAAAGGAC AGTTAGACTG TGTTATAACA CTGCACAAAG ATCAAGATCT GATTAAGGAT 
CCACGAAATC TATTGGCTAA TCAAACTTTA GTATATAGTC AAGATCTGGG GGAGATGACT 
AAACTTAATT CAAAGCGAGT ATCTTTTAAG CTTCCAAAGG ATCAAATGAA AGTCTATGTT 
GATGACATTT ATGTTATTCC TCAGCCTCAT TTCTCAACCG ACCAACCTCC ATTACCTAAA 
AAAGGACAGA GTAGTATCAA TAAAGAAGAA GTAATACTGT CTAAAGCTGG AAATAAGAGT 
TTAAATATTA TAGAAAATTC CTCTGCACCC ATATGTGAAA ACAAGCCCAA AATACTCAAT 
AGTGAGGAAT GGTTTGCTGC AGCCTGTAAA AAAGAACTGA AGGAAAATAT TCAAACAACT 
AACTATAATA CAGCTCTAGA TTTCCACAGT AACTCAGACG TAACTAAGCA AGTCATTCAA 
ACTCATGTCA ATGCTGGAGA AGCACCAGAT CCTGTAATTA CATCTAATGT TCCATGTTTT 
CATAGTATCA AACCAAATCT GAATAATTTG AATGGAAAAA CTGGAGAGTT TTTAGCCTTT 
CAAACTGTTC ATCTACCACC CCTTCCAGAG CAATTACTTG AATTAGGAAA TAAGGCACAC 
AATGATATGC ATATAGTGCA AGCTACAGAA ATACATAATA TTAACATAAT CTCCAGCAAT 
GCTAAAGATA GTAGAGATGA GGAAAATAAA AAGTCTCATA ATGGAGCTGA AACCACCTCT 
CTACCGCCAA AGACAGTTTT TAAAGATAAA GTAAGGAGAT GTTCTTTGGG AATCTTTTTG 
CCTAGATTGC CCAACAAGAG AAATTGTAGT GTCACTGGTA TTGATGACCT GGAACAGATT 
CCAGCAGACA CAACTGATAT AAATCACTTA GAAACTCAGC CGGTCTCTAG CAAAGATTCA 
GGCATTGGAT CTGTTGCAGG TAAACTGAAC CTAAGTCCTT CTCAATATAT AAATGAGGAA 
AATCTTCCTG TATATCCTGA TGAGATCAAT TCTTCAGACT CTATTAACAT AGAAACTGAG 
GAAAAGGCCT TGATTGAGAC ATACCAAAAA GAGATTTCAC CATATGAAAA TAAAATGGGA 
AAAACTTGCA ATAGCCAAAA AAGAACGTGG GTACAAGAAG AAGAAGATAT TCATAAGGAG 
AAAAAAATCA GAAAAAATGA GATTAAGTTT AGTGATACGA CACAAGATCG GGAGATTTTT 
GATCACCATA CTGAAGAGGA TATAGATAAA AGTGCTAACA GTGTATTGAT AAAAAACCTG 
AGCAGGACCC CATCTAGTTG CAGCAGCTCT CTGGATTCAA TCAAGGCTGA TGGGACCTCT 
CTGGACTTCA GCACTTACCG CAGTAGTCAA ATGGAATCAC AGTTTCTCAG AGATACTATT 
TGTGAAGAGA GCTTGAGGGA GAAACTCCAA GATGGGAGAA TAACAATAAG GGAGTTCTTT 
ATACTTCTCC AGGTCCACAT CTTGATACAG AAACCCCGAC AGAGCAATCT CCCAGGCAAT 
TTTACTGTAA ACACACCACC TACTCCAGAA GACCTGATGT TAAGTCAATA TGTTTACCGA 
CCCAAGATAC AGATTTATAG AGAAGATTGT GAGGCTCGTC GCCAAAAGAT TGAAGAATTA 
AAGCTTTCTG CATCGAACCA AGATAAGCTG TTGGTTGATA TAAATAAGAA CCTGTGGGAA 
AAAATGAGAC ACTGCTCTGA CAAAGAGCTG AAGGCCTTTG GAATTTATCT TAACAAAATA 
AAGTCATGTT TTACCAAGAT GACTAAAGTC TTCACTCACC AAGGAAAAGT GGCTCTGTAT 
GGCAAGCTGG TGCAGTCAGC TCAGAATGAG AGGGAGAAAC TTCAAATAAA GATAGATGAG 
ATGGATAAAA TACTTAAGAA GATCGATAAC TGCCTCACTG AGATGGAAAC AGAAACTAAG 
AATTTGGAGG ATGAAGAGAA AAACAATCCT GTGGAAGAAT GGGATTCTGA AATGAGAGCT 
GCAGAAAAAG AATTGGAACA GCTGAAAACT GAAGAAGAGG AGCTTCAAAG AAATCTCTTA 
GAACTGGAGG TACAAAAAGA GCAGACCCTT GCTCAAATAG ACTTTATGCA AAAACAAAGA 
AATAGAACTG AAGAGCTACT GGATCAGTTG AGCTTGTCTG AGTGGGATGT CGTTGAGTGG 
AGTGATGATC AAGCTGTATT CACCTTTGTT TATGACACGA TACAACTCAC CATCACCTTT 
GAAGAGTCAG TTGTTGGTTT CCCTTTCCTG GACAAGCGTT ATAGGAAGAT TGTTGATGTC 
AATTTTCAAT CTCTGTTAGA TGAGGATCAA GCTCCTCCTT CCTCCCTTTT AGTTCATAAG 
CTTATTTTCC AGTACGTTGA AGAAAAGGAA TCCTGGAAGA AGACATGTAC AACCCAGCAT 
CAGTTACCCA AGATGCTTGA AGAATTCTCA CTGGTAGTGC ACCATTGCAG ACTCCTTGGA 
GAGGAGATTG AGTATTTAAA GAGATGGGGA CCAAATTATA ACCTAATGAA CATAGATATT 
AATAATAATG AATTGAGACT TTTATTCTCT AGCTCCGCAG CATTTGCAAA GTTTGAAATA 
ACTTTGTTTC TCTCAGCCTA TTATCCATCT GTACCATTAC CTTCCACCAT TCAGAATCAC 
GTTGGGAACA CTAGCCAAGA TGATATTGCT ACCATTCTAT CTAAAGTGCC ACTGGAGAAC 
AACTACCTGA AGAATGTAGT CAAGCAAATT TACCAAGATC TGTTTCAGGA CTGCCATTTC 
TACCACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733468.3
CDS	162..7190
Translation	MDGVSSEANEENDNIERPVRRRHSSILKPPRSPLQDLRGGNERVQESNALRNKKNSRRVSFADTIKVFQTESHMKIVRKSEMEGCSAMVPSQLQLLPPGFKRFSCLSLPETETGENLLLIQNKKLEDNYCEITGMNTLLSAPIHTQMQQKEFSIIEHTRERKHANDQTVIFSDENQMDLTSSHTVMITKGLLDNPISEKSTKIDTTSFLANLKLHTEDSRMKKEVNFSVDQNTSSENKIDFNDFIKRLKTGKCSAFPDVPDKENFEIPIYSKEPNSASSTHQMHVSLKEDENNSNITRLFREKDDGMNFTQCHTANIQTLIPTSSETNSRESKGNDITIYGNDFMDLTFNHTLQILPATGNFSEIENQTQNAMDVTTGYGTKASGNKTVFKSKQNTAFQDLSINSADKIHITRSHIMGAETHIVSQTCNQDARILAMTPESIYSNPSIQGCKTVFYSSCNDAMEMTKCLSNMREEKNLLKHDSNYAKMYCNPDAMSSLTEKTIYSGEENMDITKSHTVAIDNQIFKQDQSNVQIAAAPTPEKEMMLQNLMTTSEDGKMNVNCNSVPHVSKERIQQSLSNPLSISLTDRKTELLSGENMDLTESHTSNLGSQVPLAAYNLAPESTSESHSQSKSSSDECEEITKSRNEPFQRSDIIAKNSLTDTWNKDKDWVLKILPYLDKDSPQSADCNQEIATSHNIVYCGGVLDKQITNRNTVSWEQSLFSTTKPLFSSGQFSMKNHDTAISSHTVKSVLGQNSKLAEPLRKSLSNPTPDYCHDKMIICSEEEQNMDLTKSHTVVIGFGPSELQELGKTNLEHTTGQLTTMNRQIAVKVEKCGKSPIEKSGVLKSNCIMDVLEDESVQKPKFPKEKQNVKIWGRKSVGGPKIDKTIVFSEDDKNDMDITKSYTIEINHRPLLEKRDCHLVPLAGTSETILYTCRQDDMEITRSHTTALECKTVSPDEITTRPMDKTVVFVDNHVELEMTESHTVFIDYQEKERTDRPNFELSQRKSLGTPTVICTPTEESVFFPGNGESDRLVANDSQLTPLEEWSNNRGPVEVADNMELSKSATCKNIKDVQSPGFLNEPLSSKSQRRKSLKLKNDKTIVFSENHKNDMDITQSCMVEIDNESALEDKEDFHLAGASKTILYSCGQDDMEITRSHTTALECKTLLPNEIAIRPMDKTVLFTDNYSDLEVTDSHTVFIDCQATEKILEENPKFGIGKGKNLGVSFPKDNSCVQEIAEKQALAVGNKIVLHTEQKQQLFAATNRTTNEIIKFHSAAMDEKVIGKVVDQACTLEKAQVESCQLNNRDRRNVDFTSSHATAVCGSSDNYSCLPNVISCTDNLEGSAMLLCDKDEEKANYCPVQNDLAYANDFASEYYLESEGQPLSAPCPLLEKEEVIQTSTKGQLDCVITLHKDQDLIKDPRNLLANQTLVYSQDLGEMTKLNSKRVSFKLPKDQMKVYVDDIYVIPQPHFSTDQPPLPKKGQSSINKEEVILSKAGNKSLNIIENSSAPICENKPKILNSEEWFAAACKKELKENIQTTNYNTALDFHSNSDVTKQVIQTHVNAGEAPDPVITSNVPCFHSIKPNLNNLNGKTGEFLAFQTVHLPPLPEQLLELGNKAHNDMHIVQATEIHNINIISSNAKDSRDEENKKSHNGAETTSLPPKTVFKDKVRRCSLGIFLPRLPNKRNCSVTGIDDLEQIPADTTDINHLETQPVSSKDSGIGSVAGKLNLSPSQYINEENLPVYPDEINSSDSINIETEEKALIETYQKEISPYENKMGKTCNSQKRTWVQEEEDIHKEKKIRKNEIKFSDTTQDREIFDHHTEEDIDKSANSVLIKNLSRTPSSCSSSLDSIKADGTSLDFSTYRSSQMESQFLRDTICEESLREKLQDGRITIREFFILLQVHILIQKPRQSNLPGNFTVNTPPTPEDLMLSQYVYRPKIQIYREDCEARRQKIEELKLSASNQDKLLVDINKNLWEKMRHCSDKELKAFGIYLNKIKSCFTKMTKVFTHQGKVALYGKLVQSAQNEREKLQIKIDEMDKILKKIDNCLTEMETETKNLEDEEKNNPVEEWDSEMRAAEKELEQLKTEEEELQRNLLELEVQKEQTLAQIDFMQKQRNRTEELLDQLSLSEWDVVEWSDDQAVFTFVYDTIQLTITFEESVVGFPFLDKRYRKIVDVNFQSLLDEDQAPPSSLLVHKLIFQYVEEKESWKKTCTTQHQLPKMLEEFSLVVHHCRLLGEEIEYLKRWGPNYNLMNIDINNNELRLLFSSSAAFAKFEITLFLSAYYPSVPLPSTIQNHVGNTSQDDIATILSKVPLENNYLKNVVKQIYQDLFQDCHFYH

Target ORF information:

RefSeq Version	NM_170589.5
Organism	Homo sapiens(human)
Definition	Homo sapiens kinetochore scaffold 1 (KNL1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170589.5

ORF Insert Sequence:

ATGGATGGGG TGTCTTCAGA GGCTAATGAA GAAAATGACA ATATAGAGAG ACCTGTTAGA 
AGACGGCATT CTTCAATATT GAAACCCCCA AGGAGTCCTC TTCAGGACCT CAGAGGTGGG 
AATGAAAGAG TTCAGGAATC CAATGCTTTG AGAAATAAGA AAAACTCTCG TCGAGTCAGC 
TTTGCAGATA CTATAAAGGT ATTCCAGACG GAGTCTCATA TGAAAATAGT GAGAAAGTCA 
GAAATGGAAG GCTGCAGTGC AATGGTGCCA TCTCAGCTGC AACTTCTGCC TCCTGGGTTT 
AAGCGATTCT CCTGCCTCAG CCTCCCAGAA ACAGAAACAG GAGAAAATCT TCTTTTGATA 
CAGAATAAGA AATTAGAAGA TAATTACTGT GAAATTACTG GGATGAACAC ATTGCTTTCT 
GCTCCCATTC ATACCCAGAT GCAACAGAAG GAGTTTTCAA TTATAGAACA TACCCGTGAA 
AGGAAACATG CAAATGACCA GACAGTCATT TTTTCAGATG AAAACCAGAT GGACCTGACA 
TCAAGTCACA CTGTAATGAT TACCAAAGGC CTTTTAGATA ATCCCATAAG TGAAAAGTCC 
ACCAAGATAG ATACCACATC ATTTCTAGCT AATTTAAAGC TTCACACCGA GGACTCAAGA 
ATGAAAAAAG AAGTAAATTT TTCCGTGGAT CAAAACACTT CTTCAGAAAA TAAAATAGAT 
TTCAATGACT TCATAAAAAG ATTGAAAACA GGAAAATGTA GTGCTTTTCC TGATGTGCCT 
GATAAAGAAA ATTTTGAGAT ACCTATTTAT TCCAAGGAAC CGAACAGTGC CTCTTCTACA 
CATCAAATGC ATGTATCTCT TAAGGAAGAT GAAAATAACA GTAATATTAC TAGGCTCTTT 
AGAGAAAAAG ATGATGGGAT GAATTTCACC CAGTGTCATA CAGCCAATAT TCAGACATTG 
ATTCCCACAT CCAGTGAGAC CAACTCACGG GAATCTAAAG GTAATGATAT TACAATTTAT 
GGCAATGACT TTATGGACTT GACATTTAAC CACACTTTGC AGATCTTACC TGCAACAGGT 
AATTTTTCTG AAATAGAAAA TCAAACTCAG AATGCCATGG ATGTAACAAC AGGTTATGGA 
ACTAAAGCTT CAGGAAATAA AACAGTTTTT AAGAGTAAAC AAAATACTGC TTTTCAAGAC 
CTTTCCATAA ACTCTGCAGA CAAAATACAT ATTACCAGAA GTCATATTAT GGGGGCAGAA 
ACTCACATAG TCTCACAGAC TTGTAATCAG GATGCCAGAA TATTAGCCAT GACCCCAGAA 
TCTATATATT CTAATCCATC TATTCAAGGT TGTAAGACTG TTTTCTATTC TAGTTGTAAT 
GATGCCATGG AAATGACCAA ATGTCTCTCA AATATGAGAG AGGAGAAAAA TTTGCTAAAG 
CATGACAGTA ATTATGCTAA AATGTATTGC AATCCAGATG CTATGTCTTC TCTCACAGAG 
AAAACTATTT ATTCCGGAGA GGAGAACATG GACATTACCA AGAGTCATAC AGTTGCAATA 
GATAATCAAA TTTTTAAACA AGATCAATCA AATGTGCAAA TAGCAGCTGC ACCAACACCC 
GAAAAAGAAA TGATGCTCCA AAATCTTATG ACCACATCAG AAGATGGGAA AATGAATGTA 
AATTGTAACT CAGTTCCTCA TGTATCTAAG GAAAGAATAC AGCAGAGCCT GTCAAATCCT 
TTGTCTATTT CATTGACTGA TAGAAAGACT GAACTCTTAT CAGGTGAAAA TATGGATTTG 
ACTGAAAGTC ACACAAGTAA CTTAGGAAGT CAGGTTCCTC TTGCAGCTTA TAATCTAGCA 
CCGGAGAGTA CCAGTGAATC TCACTCTCAG AGCAAAAGCT CTTCAGATGA ATGTGAAGAA 
ATTACCAAAA GTCGTAATGA ACCATTTCAG CGATCAGACA TAATAGCCAA AAACAGCTTA 
ACCGACACCT GGAACAAAGA CAAAGATTGG GTTTTGAAGA TTTTGCCCTA CCTTGATAAA 
GATTCTCCTC AGTCAGCTGA TTGTAATCAG GAGATAGCAA CAAGCCATAA TATAGTCTAC 
TGTGGTGGAG TTCTTGATAA ACAAATAACT AATAGAAATA CAGTATCATG GGAACAATCT 
TTGTTTTCTA CCACAAAGCC ATTATTTTCA TCAGGACAGT TCTCTATGAA AAATCATGAT 
ACTGCTATAA GTAGTCATAC AGTGAAATCT GTACTAGGCC AGAATTCTAA ACTGGCTGAG 
CCACTGAGGA AAAGTTTAAG CAATCCCACA CCTGACTATT GCCATGACAA GATGATTATA 
TGTTCAGAGG AAGAGCAAAA TATGGATCTA ACAAAGAGCC ACACTGTCGT CATTGGATTT 
GGTCCTTCTG AACTACAAGA ACTTGGTAAA ACTAATTTAG AACACACTAC TGGCCAGCTA 
ACAACAATGA ACAGACAGAT AGCTGTAAAA GTTGAAAAAT GTGGTAAAAG TCCCATAGAA 
AAAAGTGGAG TGCTTAAATC TAACTGTATT ATGGATGTGT TAGAGGACGA AAGTGTACAG 
AAACCTAAAT TTCCAAAGGA AAAGCAAAAT GTCAAAATTT GGGGAAGGAA AAGTGTTGGT 
GGACCAAAAA TTGATAAGAC TATTGTATTT TCAGAAGACG ATAAGAATGA TATGGATATC 
ACTAAGAGTT ATACAATAGA AATAAACCAT AGACCTTTAT TAGAGAAACG TGATTGTCAT 
TTGGTGCCAT TGGCAGGAAC TTCTGAAACT ATTTTATATA CATGTAGGCA GGATGACATG 
GAGATCACTA GAAGTCACAC AACTGCCTTA GAATGTAAAA CTGTCTCACC AGATGAAATA 
ACTACTAGGC CTATGGACAA AACTGTAGTG TTTGTAGATA ATCATGTTGA ACTAGAAATG 
ACAGAGTCCC ATACTGTTTT CATTGACTAC CAAGAAAAGG AAAGAACAGA CAGACCTAAC 
TTTGAACTAT CCCAAAGGAA AAGCCTAGGA ACACCAACAG TGATATGTAC TCCTACTGAG 
GAGAGTGTTT TCTTTCCAGG AAATGGTGAA AGTGACCGTC TAGTAGCAAA TGACAGCCAG 
CTAACCCCTC TGGAGGAATG GTCTAATAAT AGGGGCCCTG TAGAGGTAGC TGATAACATG 
GAATTGTCTA AATCAGCCAC TTGCAAAAAC ATCAAAGATG TACAAAGTCC TGGATTTCTG 
AATGAACCTC TATCAAGCAA AAGTCAGAGA AGAAAAAGCC TTAAGCTAAA AAATGACAAG 
ACCATTGTAT TTTCAGAGAA TCATAAAAAT GATATGGATA TTACCCAGAG TTGTATGGTG 
GAAATAGATA ACGAAAGTGC CCTGGAGGAT AAAGAGGACT TCCATTTGGC AGGGGCTTCT 
AAAACTATTT TGTATTCATG TGGGCAGGAT GACATGGAGA TCACTAGGAG TCACACAACT 
GCCTTAGAAT GTAAAACTCT CCTGCCAAAT GAAATAGCTA TTAGGCCCAT GGACAAAACC 
GTATTGTTCA CAGATAATTA CAGTGATCTG GAAGTCACCG ATTCCCATAC TGTTTTCATT 
GACTGTCAAG CCACAGAGAA AATACTTGAA GAAAACCCTA AATTTGGAAT AGGAAAAGGA 
AAAAACTTGG GTGTTTCCTT TCCTAAGGAT AATAGCTGTG TTCAAGAAAT CGCTGAAAAA 
CAAGCACTGG CTGTAGGAAA CAAAATAGTT CTTCACACCG AGCAAAAGCA ACAACTCTTT 
GCTGCTACTA ATAGAACTAC TAATGAAATC ATCAAATTTC ATAGTGCTGC TATGGATGAA 
AAGGTCATAG GGAAAGTTGT AGACCAGGCC TGTACATTGG AAAAAGCGCA AGTTGAAAGC 
TGTCAGTTAA ATAATAGAGA TAGAAGAAAT GTGGACTTTA CAAGTAGTCA TGCAACTGCT 
GTTTGTGGAT CCAGTGATAA TTATTCCTGT TTACCAAATG TTATTTCCTG TACTGATAAT 
TTGGAGGGTA GTGCCATGCT CTTATGTGAT AAAGATGAGG AAAAAGCCAA TTATTGCCCA 
GTGCAAAATG ATCTTGCTTA TGCAAATGAT TTTGCCAGTG AATATTACTT GGAATCTGAG 
GGACAGCCTC TCTCTGCTCC TTGTCCTTTG TTAGAGAAGG AAGAAGTTAT TCAAACCAGT 
ACCAAAGGAC AGTTAGACTG TGTTATAACA CTGCACAAAG ATCAAGATCT GATTAAGGAT 
CCACGAAATC TATTGGCTAA TCAAACTTTA GTATATAGTC AAGATCTGGG GGAGATGACT 
AAACTTAATT CAAAGCGAGT ATCTTTTAAG CTTCCAAAGG ATCAAATGAA AGTCTATGTT 
GATGACATTT ATGTTATTCC TCAGCCTCAT TTCTCAACCG ACCAACCTCC ATTACCTAAA 
AAAGGACAGA GTAGTATCAA TAAAGAAGAA GTAATACTGT CTAAAGCTGG AAATAAGAGT 
TTAAATATTA TAGAAAATTC CTCTGCACCC ATATGTGAAA ACAAGCCCAA AATACTCAAT 
AGTGAGGAAT GGTTTGCTGC AGCCTGTAAA AAAGAACTGA AGGAAAATAT TCAAACAACT 
AACTATAATA CAGCTCTAGA TTTCCACAGT AACTCAGACG TAACTAAGCA AGTCATTCAA 
ACTCATGTCA ATGCTGGAGA AGCACCAGAT CCTGTAATTA CATCTAATGT TCCATGTTTT 
CATAGTATCA AACCAAATCT GAATAATTTG AATGGAAAAA CTGGAGAGTT TTTAGCCTTT 
CAAACTGTTC ATCTACCACC CCTTCCAGAG CAATTACTTG AATTAGGAAA TAAGGCACAC 
AATGATATGC ATATAGTGCA AGCTACAGAA ATACATAATA TTAACATAAT CTCCAGCAAT 
GCTAAAGATA GTAGAGATGA GGAAAATAAA AAGTCTCATA ATGGAGCTGA AACCACCTCT 
CTACCGCCAA AGACAGTTTT TAAAGATAAA GTAAGGAGAT GTTCTTTGGG AATCTTTTTG 
CCTAGATTGC CCAACAAGAG AAATTGTAGT GTCACTGGTA TTGATGACCT GGAACAGATT 
CCAGCAGACA CAACTGATAT AAATCACTTA GAAACTCAGC CGGTCTCTAG CAAAGATTCA 
GGCATTGGAT CTGTTGCAGG TAAACTGAAC CTAAGTCCTT CTCAATATAT AAATGAGGAA 
AATCTTCCTG TATATCCTGA TGAGATCAAT TCTTCAGACT CTATTAACAT AGAAACTGAG 
GAAAAGGCCT TGATTGAGAC ATACCAAAAA GAGATTTCAC CATATGAAAA TAAAATGGGA 
AAAACTTGCA ATAGCCAAAA AAGAACGTGG GTACAAGAAG AAGAAGATAT TCATAAGGAG 
AAAAAAATCA GAAAAAATGA GATTAAGTTT AGTGATACGA CACAAGATCG GGAGATTTTT 
GATCACCATA CTGAAGAGGA TATAGATAAA AGTGCTAACA GTGTATTGAT AAAAAACCTG 
AGCAGGACCC CATCTAGTTG CAGCAGCTCT CTGGATTCAA TCAAGGCTGA TGGGACCTCT 
CTGGACTTCA GCACTTACCG CAGTAGTCAA ATGGAATCAC AGTTTCTCAG AGATACTATT 
TGTGAAGAGA GCTTGAGGGA GAAACTCCAA GATGGGAGAA TAACAATAAG GGAGTTCTTT 
ATACTTCTCC AGGTCCACAT CTTGATACAG AAACCCCGAC AGAGCAATCT CCCAGGCAAT 
TTTACTGTAA ACACACCACC TACTCCAGAA GACCTGATGT TAAGTCAATA TGTTTACCGA 
CCCAAGATAC AGATTTATAG AGAAGATTGT GAGGCTCGTC GCCAAAAGAT TGAAGAATTA 
AAGCTTTCTG CATCGAACCA AGATAAGCTG TTGGTTGATA TAAATAAGAA CCTGTGGGAA 
AAAATGAGAC ACTGCTCTGA CAAAGAGCTG AAGGCCTTTG GAATTTATCT TAACAAAATA 
AAGTCATGTT TTACCAAGAT GACTAAAGTC TTCACTCACC AAGGAAAAGT GGCTCTGTAT 
GGCAAGCTGG TGCAGTCAGC TCAGAATGAG AGGGAGAAAC TTCAAATAAA GATAGATGAG 
ATGGATAAAA TACTTAAGAA GATCGATAAC TGCCTCACTG AGATGGAAAC AGAAACTAAG 
AATTTGGAGG ATGAAGAGAA AAACAATCCT GTGGAAGAAT GGGATTCTGA AATGAGAGCT 
GCAGAAAAAG AATTGGAACA GCTGAAAACT GAAGAAGAGG AGCTTCAAAG AAATCTCTTA 
GAACTGGAGG TACAAAAAGA GCAGACCCTT GCTCAAATAG ACTTTATGCA AAAACAAAGA 
AATAGAACTG AAGAGCTACT GGATCAGTTG AGCTTGTCTG AGTGGGATGT CGTTGAGTGG 
AGTGATGATC AAGCTGTATT CACCTTTGTT TATGACACGA TACAACTCAC CATCACCTTT 
GAAGAGTCAG TTGTTGGTTT CCCTTTCCTG GACAAGCGTT ATAGGAAGAT TGTTGATGTC 
AATTTTCAAT CTCTGTTAGA TGAGGATCAA GCTCCTCCTT CCTCCCTTTT AGTTCATAAG 
CTTATTTTCC AGTACGTTGA AGAAAAGGAA TCCTGGAAGA AGACATGTAC AACCCAGCAT 
CAGTTACCCA AGATGCTTGA AGAATTCTCA CTGGTAGTGC ACCATTGCAG ACTCCTTGGA 
GAGGAGATTG AGTATTTAAA GAGATGGGGA CCAAATTATA ACCTAATGAA CATAGATATT 
AATAATAATG AATTGAGACT TTTATTCTCT AGCTCCGCAG CATTTGCAAA GTTTGAAATA 
ACTTTGTTTC TCTCAGCCTA TTATCCATCT GTACCATTAC CTTCCACCAT TCAGAATCAC 
GTTGGGAACA CTAGCCAAGA TGATATTGCT ACCATTCTAT CTAAAGTGCC ACTGGAGAAC 
AACTACCTGA AGAATGTAGT CAAGCAAATT TACCAAGATC TGTTTCAGGA CTGCCATTTC 
TACCACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KNL1 ( NM_170589.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_733468.3 Homo sapiens kinetochore scaffold 1 (KNL1), transcript variant 1, mRNA.

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CloneID	OHu20484
Clone ID Related Accession (Same CDS sequence)	NM_170589.5 , NM_170589.4
Accession Version	NM_170589.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7029bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	kinetochore scaffold 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC014306.1, AF461041.1, AF173994.1, AC022405.5 and AB046790.1. This sequence is a reference standard in the RefSeqGene project. On Jan 30, 2013 this sequence version replaced NM_170589.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF173994.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968968, SAMEA2142586 [ECO:0000350] ##Evidence-Data-END##

ATGGATGGGG TGTCTTCAGA GGCTAATGAA GAAAATGACA ATATAGAGAG ACCTGTTAGA 
AGACGGCATT CTTCAATATT GAAACCCCCA AGGAGTCCTC TTCAGGACCT CAGAGGTGGG 
AATGAAAGAG TTCAGGAATC CAATGCTTTG AGAAATAAGA AAAACTCTCG TCGAGTCAGC 
TTTGCAGATA CTATAAAGGT ATTCCAGACG GAGTCTCATA TGAAAATAGT GAGAAAGTCA 
GAAATGGAAG GCTGCAGTGC AATGGTGCCA TCTCAGCTGC AACTTCTGCC TCCTGGGTTT 
AAGCGATTCT CCTGCCTCAG CCTCCCAGAA ACAGAAACAG GAGAAAATCT TCTTTTGATA 
CAGAATAAGA AATTAGAAGA TAATTACTGT GAAATTACTG GGATGAACAC ATTGCTTTCT 
GCTCCCATTC ATACCCAGAT GCAACAGAAG GAGTTTTCAA TTATAGAACA TACCCGTGAA 
AGGAAACATG CAAATGACCA GACAGTCATT TTTTCAGATG AAAACCAGAT GGACCTGACA 
TCAAGTCACA CTGTAATGAT TACCAAAGGC CTTTTAGATA ATCCCATAAG TGAAAAGTCC 
ACCAAGATAG ATACCACATC ATTTCTAGCT AATTTAAAGC TTCACACCGA GGACTCAAGA 
ATGAAAAAAG AAGTAAATTT TTCCGTGGAT CAAAACACTT CTTCAGAAAA TAAAATAGAT 
TTCAATGACT TCATAAAAAG ATTGAAAACA GGAAAATGTA GTGCTTTTCC TGATGTGCCT 
GATAAAGAAA ATTTTGAGAT ACCTATTTAT TCCAAGGAAC CGAACAGTGC CTCTTCTACA 
CATCAAATGC ATGTATCTCT TAAGGAAGAT GAAAATAACA GTAATATTAC TAGGCTCTTT 
AGAGAAAAAG ATGATGGGAT GAATTTCACC CAGTGTCATA CAGCCAATAT TCAGACATTG 
ATTCCCACAT CCAGTGAGAC CAACTCACGG GAATCTAAAG GTAATGATAT TACAATTTAT 
GGCAATGACT TTATGGACTT GACATTTAAC CACACTTTGC AGATCTTACC TGCAACAGGT 
AATTTTTCTG AAATAGAAAA TCAAACTCAG AATGCCATGG ATGTAACAAC AGGTTATGGA 
ACTAAAGCTT CAGGAAATAA AACAGTTTTT AAGAGTAAAC AAAATACTGC TTTTCAAGAC 
CTTTCCATAA ACTCTGCAGA CAAAATACAT ATTACCAGAA GTCATATTAT GGGGGCAGAA 
ACTCACATAG TCTCACAGAC TTGTAATCAG GATGCCAGAA TATTAGCCAT GACCCCAGAA 
TCTATATATT CTAATCCATC TATTCAAGGT TGTAAGACTG TTTTCTATTC TAGTTGTAAT 
GATGCCATGG AAATGACCAA ATGTCTCTCA AATATGAGAG AGGAGAAAAA TTTGCTAAAG 
CATGACAGTA ATTATGCTAA AATGTATTGC AATCCAGATG CTATGTCTTC TCTCACAGAG 
AAAACTATTT ATTCCGGAGA GGAGAACATG GACATTACCA AGAGTCATAC AGTTGCAATA 
GATAATCAAA TTTTTAAACA AGATCAATCA AATGTGCAAA TAGCAGCTGC ACCAACACCC 
GAAAAAGAAA TGATGCTCCA AAATCTTATG ACCACATCAG AAGATGGGAA AATGAATGTA 
AATTGTAACT CAGTTCCTCA TGTATCTAAG GAAAGAATAC AGCAGAGCCT GTCAAATCCT 
TTGTCTATTT CATTGACTGA TAGAAAGACT GAACTCTTAT CAGGTGAAAA TATGGATTTG 
ACTGAAAGTC ACACAAGTAA CTTAGGAAGT CAGGTTCCTC TTGCAGCTTA TAATCTAGCA 
CCGGAGAGTA CCAGTGAATC TCACTCTCAG AGCAAAAGCT CTTCAGATGA ATGTGAAGAA 
ATTACCAAAA GTCGTAATGA ACCATTTCAG CGATCAGACA TAATAGCCAA AAACAGCTTA 
ACCGACACCT GGAACAAAGA CAAAGATTGG GTTTTGAAGA TTTTGCCCTA CCTTGATAAA 
GATTCTCCTC AGTCAGCTGA TTGTAATCAG GAGATAGCAA CAAGCCATAA TATAGTCTAC 
TGTGGTGGAG TTCTTGATAA ACAAATAACT AATAGAAATA CAGTATCATG GGAACAATCT 
TTGTTTTCTA CCACAAAGCC ATTATTTTCA TCAGGACAGT TCTCTATGAA AAATCATGAT 
ACTGCTATAA GTAGTCATAC AGTGAAATCT GTACTAGGCC AGAATTCTAA ACTGGCTGAG 
CCACTGAGGA AAAGTTTAAG CAATCCCACA CCTGACTATT GCCATGACAA GATGATTATA 
TGTTCAGAGG AAGAGCAAAA TATGGATCTA ACAAAGAGCC ACACTGTCGT CATTGGATTT 
GGTCCTTCTG AACTACAAGA ACTTGGTAAA ACTAATTTAG AACACACTAC TGGCCAGCTA 
ACAACAATGA ACAGACAGAT AGCTGTAAAA GTTGAAAAAT GTGGTAAAAG TCCCATAGAA 
AAAAGTGGAG TGCTTAAATC TAACTGTATT ATGGATGTGT TAGAGGACGA AAGTGTACAG 
AAACCTAAAT TTCCAAAGGA AAAGCAAAAT GTCAAAATTT GGGGAAGGAA AAGTGTTGGT 
GGACCAAAAA TTGATAAGAC TATTGTATTT TCAGAAGACG ATAAGAATGA TATGGATATC 
ACTAAGAGTT ATACAATAGA AATAAACCAT AGACCTTTAT TAGAGAAACG TGATTGTCAT 
TTGGTGCCAT TGGCAGGAAC TTCTGAAACT ATTTTATATA CATGTAGGCA GGATGACATG 
GAGATCACTA GAAGTCACAC AACTGCCTTA GAATGTAAAA CTGTCTCACC AGATGAAATA 
ACTACTAGGC CTATGGACAA AACTGTAGTG TTTGTAGATA ATCATGTTGA ACTAGAAATG 
ACAGAGTCCC ATACTGTTTT CATTGACTAC CAAGAAAAGG AAAGAACAGA CAGACCTAAC 
TTTGAACTAT CCCAAAGGAA AAGCCTAGGA ACACCAACAG TGATATGTAC TCCTACTGAG 
GAGAGTGTTT TCTTTCCAGG AAATGGTGAA AGTGACCGTC TAGTAGCAAA TGACAGCCAG 
CTAACCCCTC TGGAGGAATG GTCTAATAAT AGGGGCCCTG TAGAGGTAGC TGATAACATG 
GAATTGTCTA AATCAGCCAC TTGCAAAAAC ATCAAAGATG TACAAAGTCC TGGATTTCTG 
AATGAACCTC TATCAAGCAA AAGTCAGAGA AGAAAAAGCC TTAAGCTAAA AAATGACAAG 
ACCATTGTAT TTTCAGAGAA TCATAAAAAT GATATGGATA TTACCCAGAG TTGTATGGTG 
GAAATAGATA ACGAAAGTGC CCTGGAGGAT AAAGAGGACT TCCATTTGGC AGGGGCTTCT 
AAAACTATTT TGTATTCATG TGGGCAGGAT GACATGGAGA TCACTAGGAG TCACACAACT 
GCCTTAGAAT GTAAAACTCT CCTGCCAAAT GAAATAGCTA TTAGGCCCAT GGACAAAACC 
GTATTGTTCA CAGATAATTA CAGTGATCTG GAAGTCACCG ATTCCCATAC TGTTTTCATT 
GACTGTCAAG CCACAGAGAA AATACTTGAA GAAAACCCTA AATTTGGAAT AGGAAAAGGA 
AAAAACTTGG GTGTTTCCTT TCCTAAGGAT AATAGCTGTG TTCAAGAAAT CGCTGAAAAA 
CAAGCACTGG CTGTAGGAAA CAAAATAGTT CTTCACACCG AGCAAAAGCA ACAACTCTTT 
GCTGCTACTA ATAGAACTAC TAATGAAATC ATCAAATTTC ATAGTGCTGC TATGGATGAA 
AAGGTCATAG GGAAAGTTGT AGACCAGGCC TGTACATTGG AAAAAGCGCA AGTTGAAAGC 
TGTCAGTTAA ATAATAGAGA TAGAAGAAAT GTGGACTTTA CAAGTAGTCA TGCAACTGCT 
GTTTGTGGAT CCAGTGATAA TTATTCCTGT TTACCAAATG TTATTTCCTG TACTGATAAT 
TTGGAGGGTA GTGCCATGCT CTTATGTGAT AAAGATGAGG AAAAAGCCAA TTATTGCCCA 
GTGCAAAATG ATCTTGCTTA TGCAAATGAT TTTGCCAGTG AATATTACTT GGAATCTGAG 
GGACAGCCTC TCTCTGCTCC TTGTCCTTTG TTAGAGAAGG AAGAAGTTAT TCAAACCAGT 
ACCAAAGGAC AGTTAGACTG TGTTATAACA CTGCACAAAG ATCAAGATCT GATTAAGGAT 
CCACGAAATC TATTGGCTAA TCAAACTTTA GTATATAGTC AAGATCTGGG GGAGATGACT 
AAACTTAATT CAAAGCGAGT ATCTTTTAAG CTTCCAAAGG ATCAAATGAA AGTCTATGTT 
GATGACATTT ATGTTATTCC TCAGCCTCAT TTCTCAACCG ACCAACCTCC ATTACCTAAA 
AAAGGACAGA GTAGTATCAA TAAAGAAGAA GTAATACTGT CTAAAGCTGG AAATAAGAGT 
TTAAATATTA TAGAAAATTC CTCTGCACCC ATATGTGAAA ACAAGCCCAA AATACTCAAT 
AGTGAGGAAT GGTTTGCTGC AGCCTGTAAA AAAGAACTGA AGGAAAATAT TCAAACAACT 
AACTATAATA CAGCTCTAGA TTTCCACAGT AACTCAGACG TAACTAAGCA AGTCATTCAA 
ACTCATGTCA ATGCTGGAGA AGCACCAGAT CCTGTAATTA CATCTAATGT TCCATGTTTT 
CATAGTATCA AACCAAATCT GAATAATTTG AATGGAAAAA CTGGAGAGTT TTTAGCCTTT 
CAAACTGTTC ATCTACCACC CCTTCCAGAG CAATTACTTG AATTAGGAAA TAAGGCACAC 
AATGATATGC ATATAGTGCA AGCTACAGAA ATACATAATA TTAACATAAT CTCCAGCAAT 
GCTAAAGATA GTAGAGATGA GGAAAATAAA AAGTCTCATA ATGGAGCTGA AACCACCTCT 
CTACCGCCAA AGACAGTTTT TAAAGATAAA GTAAGGAGAT GTTCTTTGGG AATCTTTTTG 
CCTAGATTGC CCAACAAGAG AAATTGTAGT GTCACTGGTA TTGATGACCT GGAACAGATT 
CCAGCAGACA CAACTGATAT AAATCACTTA GAAACTCAGC CGGTCTCTAG CAAAGATTCA 
GGCATTGGAT CTGTTGCAGG TAAACTGAAC CTAAGTCCTT CTCAATATAT AAATGAGGAA 
AATCTTCCTG TATATCCTGA TGAGATCAAT TCTTCAGACT CTATTAACAT AGAAACTGAG 
GAAAAGGCCT TGATTGAGAC ATACCAAAAA GAGATTTCAC CATATGAAAA TAAAATGGGA 
AAAACTTGCA ATAGCCAAAA AAGAACGTGG GTACAAGAAG AAGAAGATAT TCATAAGGAG 
AAAAAAATCA GAAAAAATGA GATTAAGTTT AGTGATACGA CACAAGATCG GGAGATTTTT 
GATCACCATA CTGAAGAGGA TATAGATAAA AGTGCTAACA GTGTATTGAT AAAAAACCTG 
AGCAGGACCC CATCTAGTTG CAGCAGCTCT CTGGATTCAA TCAAGGCTGA TGGGACCTCT 
CTGGACTTCA GCACTTACCG CAGTAGTCAA ATGGAATCAC AGTTTCTCAG AGATACTATT 
TGTGAAGAGA GCTTGAGGGA GAAACTCCAA GATGGGAGAA TAACAATAAG GGAGTTCTTT 
ATACTTCTCC AGGTCCACAT CTTGATACAG AAACCCCGAC AGAGCAATCT CCCAGGCAAT 
TTTACTGTAA ACACACCACC TACTCCAGAA GACCTGATGT TAAGTCAATA TGTTTACCGA 
CCCAAGATAC AGATTTATAG AGAAGATTGT GAGGCTCGTC GCCAAAAGAT TGAAGAATTA 
AAGCTTTCTG CATCGAACCA AGATAAGCTG TTGGTTGATA TAAATAAGAA CCTGTGGGAA 
AAAATGAGAC ACTGCTCTGA CAAAGAGCTG AAGGCCTTTG GAATTTATCT TAACAAAATA 
AAGTCATGTT TTACCAAGAT GACTAAAGTC TTCACTCACC AAGGAAAAGT GGCTCTGTAT 
GGCAAGCTGG TGCAGTCAGC TCAGAATGAG AGGGAGAAAC TTCAAATAAA GATAGATGAG 
ATGGATAAAA TACTTAAGAA GATCGATAAC TGCCTCACTG AGATGGAAAC AGAAACTAAG 
AATTTGGAGG ATGAAGAGAA AAACAATCCT GTGGAAGAAT GGGATTCTGA AATGAGAGCT 
GCAGAAAAAG AATTGGAACA GCTGAAAACT GAAGAAGAGG AGCTTCAAAG AAATCTCTTA 
GAACTGGAGG TACAAAAAGA GCAGACCCTT GCTCAAATAG ACTTTATGCA AAAACAAAGA 
AATAGAACTG AAGAGCTACT GGATCAGTTG AGCTTGTCTG AGTGGGATGT CGTTGAGTGG 
AGTGATGATC AAGCTGTATT CACCTTTGTT TATGACACGA TACAACTCAC CATCACCTTT 
GAAGAGTCAG TTGTTGGTTT CCCTTTCCTG GACAAGCGTT ATAGGAAGAT TGTTGATGTC 
AATTTTCAAT CTCTGTTAGA TGAGGATCAA GCTCCTCCTT CCTCCCTTTT AGTTCATAAG 
CTTATTTTCC AGTACGTTGA AGAAAAGGAA TCCTGGAAGA AGACATGTAC AACCCAGCAT 
CAGTTACCCA AGATGCTTGA AGAATTCTCA CTGGTAGTGC ACCATTGCAG ACTCCTTGGA 
GAGGAGATTG AGTATTTAAA GAGATGGGGA CCAAATTATA ACCTAATGAA CATAGATATT 
AATAATAATG AATTGAGACT TTTATTCTCT AGCTCCGCAG CATTTGCAAA GTTTGAAATA 
ACTTTGTTTC TCTCAGCCTA TTATCCATCT GTACCATTAC CTTCCACCAT TCAGAATCAC 
GTTGGGAACA CTAGCCAAGA TGATATTGCT ACCATTCTAT CTAAAGTGCC ACTGGAGAAC 
AACTACCTGA AGAATGTAGT CAAGCAAATT TACCAAGATC TGTTTCAGGA CTGCCATTTC 
TACCACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733468.3
CDS	162..7190
Translation	MDGVSSEANEENDNIERPVRRRHSSILKPPRSPLQDLRGGNERVQESNALRNKKNSRRVSFADTIKVFQTESHMKIVRKSEMEGCSAMVPSQLQLLPPGFKRFSCLSLPETETGENLLLIQNKKLEDNYCEITGMNTLLSAPIHTQMQQKEFSIIEHTRERKHANDQTVIFSDENQMDLTSSHTVMITKGLLDNPISEKSTKIDTTSFLANLKLHTEDSRMKKEVNFSVDQNTSSENKIDFNDFIKRLKTGKCSAFPDVPDKENFEIPIYSKEPNSASSTHQMHVSLKEDENNSNITRLFREKDDGMNFTQCHTANIQTLIPTSSETNSRESKGNDITIYGNDFMDLTFNHTLQILPATGNFSEIENQTQNAMDVTTGYGTKASGNKTVFKSKQNTAFQDLSINSADKIHITRSHIMGAETHIVSQTCNQDARILAMTPESIYSNPSIQGCKTVFYSSCNDAMEMTKCLSNMREEKNLLKHDSNYAKMYCNPDAMSSLTEKTIYSGEENMDITKSHTVAIDNQIFKQDQSNVQIAAAPTPEKEMMLQNLMTTSEDGKMNVNCNSVPHVSKERIQQSLSNPLSISLTDRKTELLSGENMDLTESHTSNLGSQVPLAAYNLAPESTSESHSQSKSSSDECEEITKSRNEPFQRSDIIAKNSLTDTWNKDKDWVLKILPYLDKDSPQSADCNQEIATSHNIVYCGGVLDKQITNRNTVSWEQSLFSTTKPLFSSGQFSMKNHDTAISSHTVKSVLGQNSKLAEPLRKSLSNPTPDYCHDKMIICSEEEQNMDLTKSHTVVIGFGPSELQELGKTNLEHTTGQLTTMNRQIAVKVEKCGKSPIEKSGVLKSNCIMDVLEDESVQKPKFPKEKQNVKIWGRKSVGGPKIDKTIVFSEDDKNDMDITKSYTIEINHRPLLEKRDCHLVPLAGTSETILYTCRQDDMEITRSHTTALECKTVSPDEITTRPMDKTVVFVDNHVELEMTESHTVFIDYQEKERTDRPNFELSQRKSLGTPTVICTPTEESVFFPGNGESDRLVANDSQLTPLEEWSNNRGPVEVADNMELSKSATCKNIKDVQSPGFLNEPLSSKSQRRKSLKLKNDKTIVFSENHKNDMDITQSCMVEIDNESALEDKEDFHLAGASKTILYSCGQDDMEITRSHTTALECKTLLPNEIAIRPMDKTVLFTDNYSDLEVTDSHTVFIDCQATEKILEENPKFGIGKGKNLGVSFPKDNSCVQEIAEKQALAVGNKIVLHTEQKQQLFAATNRTTNEIIKFHSAAMDEKVIGKVVDQACTLEKAQVESCQLNNRDRRNVDFTSSHATAVCGSSDNYSCLPNVISCTDNLEGSAMLLCDKDEEKANYCPVQNDLAYANDFASEYYLESEGQPLSAPCPLLEKEEVIQTSTKGQLDCVITLHKDQDLIKDPRNLLANQTLVYSQDLGEMTKLNSKRVSFKLPKDQMKVYVDDIYVIPQPHFSTDQPPLPKKGQSSINKEEVILSKAGNKSLNIIENSSAPICENKPKILNSEEWFAAACKKELKENIQTTNYNTALDFHSNSDVTKQVIQTHVNAGEAPDPVITSNVPCFHSIKPNLNNLNGKTGEFLAFQTVHLPPLPEQLLELGNKAHNDMHIVQATEIHNINIISSNAKDSRDEENKKSHNGAETTSLPPKTVFKDKVRRCSLGIFLPRLPNKRNCSVTGIDDLEQIPADTTDINHLETQPVSSKDSGIGSVAGKLNLSPSQYINEENLPVYPDEINSSDSINIETEEKALIETYQKEISPYENKMGKTCNSQKRTWVQEEEDIHKEKKIRKNEIKFSDTTQDREIFDHHTEEDIDKSANSVLIKNLSRTPSSCSSSLDSIKADGTSLDFSTYRSSQMESQFLRDTICEESLREKLQDGRITIREFFILLQVHILIQKPRQSNLPGNFTVNTPPTPEDLMLSQYVYRPKIQIYREDCEARRQKIEELKLSASNQDKLLVDINKNLWEKMRHCSDKELKAFGIYLNKIKSCFTKMTKVFTHQGKVALYGKLVQSAQNEREKLQIKIDEMDKILKKIDNCLTEMETETKNLEDEEKNNPVEEWDSEMRAAEKELEQLKTEEEELQRNLLELEVQKEQTLAQIDFMQKQRNRTEELLDQLSLSEWDVVEWSDDQAVFTFVYDTIQLTITFEESVVGFPFLDKRYRKIVDVNFQSLLDEDQAPPSSLLVHKLIFQYVEEKESWKKTCTTQHQLPKMLEEFSLVVHHCRLLGEEIEYLKRWGPNYNLMNIDINNNELRLLFSSSAAFAKFEITLFLSAYYPSVPLPSTIQNHVGNTSQDDIATILSKVPLENNYLKNVVKQIYQDLFQDCHFYH

Target ORF information:

RefSeq Version	NM_170589.4
Organism	Homo sapiens(human)
Definition	Homo sapiens kinetochore scaffold 1 (KNL1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170589.4

ORF Insert Sequence:

ATGGATGGGG TGTCTTCAGA GGCTAATGAA GAAAATGACA ATATAGAGAG ACCTGTTAGA 
AGACGGCATT CTTCAATATT GAAACCCCCA AGGAGTCCTC TTCAGGACCT CAGAGGTGGG 
AATGAAAGAG TTCAGGAATC CAATGCTTTG AGAAATAAGA AAAACTCTCG TCGAGTCAGC 
TTTGCAGATA CTATAAAGGT ATTCCAGACG GAGTCTCATA TGAAAATAGT GAGAAAGTCA 
GAAATGGAAG GCTGCAGTGC AATGGTGCCA TCTCAGCTGC AACTTCTGCC TCCTGGGTTT 
AAGCGATTCT CCTGCCTCAG CCTCCCAGAA ACAGAAACAG GAGAAAATCT TCTTTTGATA 
CAGAATAAGA AATTAGAAGA TAATTACTGT GAAATTACTG GGATGAACAC ATTGCTTTCT 
GCTCCCATTC ATACCCAGAT GCAACAGAAG GAGTTTTCAA TTATAGAACA TACCCGTGAA 
AGGAAACATG CAAATGACCA GACAGTCATT TTTTCAGATG AAAACCAGAT GGACCTGACA 
TCAAGTCACA CTGTAATGAT TACCAAAGGC CTTTTAGATA ATCCCATAAG TGAAAAGTCC 
ACCAAGATAG ATACCACATC ATTTCTAGCT AATTTAAAGC TTCACACCGA GGACTCAAGA 
ATGAAAAAAG AAGTAAATTT TTCCGTGGAT CAAAACACTT CTTCAGAAAA TAAAATAGAT 
TTCAATGACT TCATAAAAAG ATTGAAAACA GGAAAATGTA GTGCTTTTCC TGATGTGCCT 
GATAAAGAAA ATTTTGAGAT ACCTATTTAT TCCAAGGAAC CGAACAGTGC CTCTTCTACA 
CATCAAATGC ATGTATCTCT TAAGGAAGAT GAAAATAACA GTAATATTAC TAGGCTCTTT 
AGAGAAAAAG ATGATGGGAT GAATTTCACC CAGTGTCATA CAGCCAATAT TCAGACATTG 
ATTCCCACAT CCAGTGAGAC CAACTCACGG GAATCTAAAG GTAATGATAT TACAATTTAT 
GGCAATGACT TTATGGACTT GACATTTAAC CACACTTTGC AGATCTTACC TGCAACAGGT 
AATTTTTCTG AAATAGAAAA TCAAACTCAG AATGCCATGG ATGTAACAAC AGGTTATGGA 
ACTAAAGCTT CAGGAAATAA AACAGTTTTT AAGAGTAAAC AAAATACTGC TTTTCAAGAC 
CTTTCCATAA ACTCTGCAGA CAAAATACAT ATTACCAGAA GTCATATTAT GGGGGCAGAA 
ACTCACATAG TCTCACAGAC TTGTAATCAG GATGCCAGAA TATTAGCCAT GACCCCAGAA 
TCTATATATT CTAATCCATC TATTCAAGGT TGTAAGACTG TTTTCTATTC TAGTTGTAAT 
GATGCCATGG AAATGACCAA ATGTCTCTCA AATATGAGAG AGGAGAAAAA TTTGCTAAAG 
CATGACAGTA ATTATGCTAA AATGTATTGC AATCCAGATG CTATGTCTTC TCTCACAGAG 
AAAACTATTT ATTCCGGAGA GGAGAACATG GACATTACCA AGAGTCATAC AGTTGCAATA 
GATAATCAAA TTTTTAAACA AGATCAATCA AATGTGCAAA TAGCAGCTGC ACCAACACCC 
GAAAAAGAAA TGATGCTCCA AAATCTTATG ACCACATCAG AAGATGGGAA AATGAATGTA 
AATTGTAACT CAGTTCCTCA TGTATCTAAG GAAAGAATAC AGCAGAGCCT GTCAAATCCT 
TTGTCTATTT CATTGACTGA TAGAAAGACT GAACTCTTAT CAGGTGAAAA TATGGATTTG 
ACTGAAAGTC ACACAAGTAA CTTAGGAAGT CAGGTTCCTC TTGCAGCTTA TAATCTAGCA 
CCGGAGAGTA CCAGTGAATC TCACTCTCAG AGCAAAAGCT CTTCAGATGA ATGTGAAGAA 
ATTACCAAAA GTCGTAATGA ACCATTTCAG CGATCAGACA TAATAGCCAA AAACAGCTTA 
ACCGACACCT GGAACAAAGA CAAAGATTGG GTTTTGAAGA TTTTGCCCTA CCTTGATAAA 
GATTCTCCTC AGTCAGCTGA TTGTAATCAG GAGATAGCAA CAAGCCATAA TATAGTCTAC 
TGTGGTGGAG TTCTTGATAA ACAAATAACT AATAGAAATA CAGTATCATG GGAACAATCT 
TTGTTTTCTA CCACAAAGCC ATTATTTTCA TCAGGACAGT TCTCTATGAA AAATCATGAT 
ACTGCTATAA GTAGTCATAC AGTGAAATCT GTACTAGGCC AGAATTCTAA ACTGGCTGAG 
CCACTGAGGA AAAGTTTAAG CAATCCCACA CCTGACTATT GCCATGACAA GATGATTATA 
TGTTCAGAGG AAGAGCAAAA TATGGATCTA ACAAAGAGCC ACACTGTCGT CATTGGATTT 
GGTCCTTCTG AACTACAAGA ACTTGGTAAA ACTAATTTAG AACACACTAC TGGCCAGCTA 
ACAACAATGA ACAGACAGAT AGCTGTAAAA GTTGAAAAAT GTGGTAAAAG TCCCATAGAA 
AAAAGTGGAG TGCTTAAATC TAACTGTATT ATGGATGTGT TAGAGGACGA AAGTGTACAG 
AAACCTAAAT TTCCAAAGGA AAAGCAAAAT GTCAAAATTT GGGGAAGGAA AAGTGTTGGT 
GGACCAAAAA TTGATAAGAC TATTGTATTT TCAGAAGACG ATAAGAATGA TATGGATATC 
ACTAAGAGTT ATACAATAGA AATAAACCAT AGACCTTTAT TAGAGAAACG TGATTGTCAT 
TTGGTGCCAT TGGCAGGAAC TTCTGAAACT ATTTTATATA CATGTAGGCA GGATGACATG 
GAGATCACTA GAAGTCACAC AACTGCCTTA GAATGTAAAA CTGTCTCACC AGATGAAATA 
ACTACTAGGC CTATGGACAA AACTGTAGTG TTTGTAGATA ATCATGTTGA ACTAGAAATG 
ACAGAGTCCC ATACTGTTTT CATTGACTAC CAAGAAAAGG AAAGAACAGA CAGACCTAAC 
TTTGAACTAT CCCAAAGGAA AAGCCTAGGA ACACCAACAG TGATATGTAC TCCTACTGAG 
GAGAGTGTTT TCTTTCCAGG AAATGGTGAA AGTGACCGTC TAGTAGCAAA TGACAGCCAG 
CTAACCCCTC TGGAGGAATG GTCTAATAAT AGGGGCCCTG TAGAGGTAGC TGATAACATG 
GAATTGTCTA AATCAGCCAC TTGCAAAAAC ATCAAAGATG TACAAAGTCC TGGATTTCTG 
AATGAACCTC TATCAAGCAA AAGTCAGAGA AGAAAAAGCC TTAAGCTAAA AAATGACAAG 
ACCATTGTAT TTTCAGAGAA TCATAAAAAT GATATGGATA TTACCCAGAG TTGTATGGTG 
GAAATAGATA ACGAAAGTGC CCTGGAGGAT AAAGAGGACT TCCATTTGGC AGGGGCTTCT 
AAAACTATTT TGTATTCATG TGGGCAGGAT GACATGGAGA TCACTAGGAG TCACACAACT 
GCCTTAGAAT GTAAAACTCT CCTGCCAAAT GAAATAGCTA TTAGGCCCAT GGACAAAACC 
GTATTGTTCA CAGATAATTA CAGTGATCTG GAAGTCACCG ATTCCCATAC TGTTTTCATT 
GACTGTCAAG CCACAGAGAA AATACTTGAA GAAAACCCTA AATTTGGAAT AGGAAAAGGA 
AAAAACTTGG GTGTTTCCTT TCCTAAGGAT AATAGCTGTG TTCAAGAAAT CGCTGAAAAA 
CAAGCACTGG CTGTAGGAAA CAAAATAGTT CTTCACACCG AGCAAAAGCA ACAACTCTTT 
GCTGCTACTA ATAGAACTAC TAATGAAATC ATCAAATTTC ATAGTGCTGC TATGGATGAA 
AAGGTCATAG GGAAAGTTGT AGACCAGGCC TGTACATTGG AAAAAGCGCA AGTTGAAAGC 
TGTCAGTTAA ATAATAGAGA TAGAAGAAAT GTGGACTTTA CAAGTAGTCA TGCAACTGCT 
GTTTGTGGAT CCAGTGATAA TTATTCCTGT TTACCAAATG TTATTTCCTG TACTGATAAT 
TTGGAGGGTA GTGCCATGCT CTTATGTGAT AAAGATGAGG AAAAAGCCAA TTATTGCCCA 
GTGCAAAATG ATCTTGCTTA TGCAAATGAT TTTGCCAGTG AATATTACTT GGAATCTGAG 
GGACAGCCTC TCTCTGCTCC TTGTCCTTTG TTAGAGAAGG AAGAAGTTAT TCAAACCAGT 
ACCAAAGGAC AGTTAGACTG TGTTATAACA CTGCACAAAG ATCAAGATCT GATTAAGGAT 
CCACGAAATC TATTGGCTAA TCAAACTTTA GTATATAGTC AAGATCTGGG GGAGATGACT 
AAACTTAATT CAAAGCGAGT ATCTTTTAAG CTTCCAAAGG ATCAAATGAA AGTCTATGTT 
GATGACATTT ATGTTATTCC TCAGCCTCAT TTCTCAACCG ACCAACCTCC ATTACCTAAA 
AAAGGACAGA GTAGTATCAA TAAAGAAGAA GTAATACTGT CTAAAGCTGG AAATAAGAGT 
TTAAATATTA TAGAAAATTC CTCTGCACCC ATATGTGAAA ACAAGCCCAA AATACTCAAT 
AGTGAGGAAT GGTTTGCTGC AGCCTGTAAA AAAGAACTGA AGGAAAATAT TCAAACAACT 
AACTATAATA CAGCTCTAGA TTTCCACAGT AACTCAGACG TAACTAAGCA AGTCATTCAA 
ACTCATGTCA ATGCTGGAGA AGCACCAGAT CCTGTAATTA CATCTAATGT TCCATGTTTT 
CATAGTATCA AACCAAATCT GAATAATTTG AATGGAAAAA CTGGAGAGTT TTTAGCCTTT 
CAAACTGTTC ATCTACCACC CCTTCCAGAG CAATTACTTG AATTAGGAAA TAAGGCACAC 
AATGATATGC ATATAGTGCA AGCTACAGAA ATACATAATA TTAACATAAT CTCCAGCAAT 
GCTAAAGATA GTAGAGATGA GGAAAATAAA AAGTCTCATA ATGGAGCTGA AACCACCTCT 
CTACCGCCAA AGACAGTTTT TAAAGATAAA GTAAGGAGAT GTTCTTTGGG AATCTTTTTG 
CCTAGATTGC CCAACAAGAG AAATTGTAGT GTCACTGGTA TTGATGACCT GGAACAGATT 
CCAGCAGACA CAACTGATAT AAATCACTTA GAAACTCAGC CGGTCTCTAG CAAAGATTCA 
GGCATTGGAT CTGTTGCAGG TAAACTGAAC CTAAGTCCTT CTCAATATAT AAATGAGGAA 
AATCTTCCTG TATATCCTGA TGAGATCAAT TCTTCAGACT CTATTAACAT AGAAACTGAG 
GAAAAGGCCT TGATTGAGAC ATACCAAAAA GAGATTTCAC CATATGAAAA TAAAATGGGA 
AAAACTTGCA ATAGCCAAAA AAGAACGTGG GTACAAGAAG AAGAAGATAT TCATAAGGAG 
AAAAAAATCA GAAAAAATGA GATTAAGTTT AGTGATACGA CACAAGATCG GGAGATTTTT 
GATCACCATA CTGAAGAGGA TATAGATAAA AGTGCTAACA GTGTATTGAT AAAAAACCTG 
AGCAGGACCC CATCTAGTTG CAGCAGCTCT CTGGATTCAA TCAAGGCTGA TGGGACCTCT 
CTGGACTTCA GCACTTACCG CAGTAGTCAA ATGGAATCAC AGTTTCTCAG AGATACTATT 
TGTGAAGAGA GCTTGAGGGA GAAACTCCAA GATGGGAGAA TAACAATAAG GGAGTTCTTT 
ATACTTCTCC AGGTCCACAT CTTGATACAG AAACCCCGAC AGAGCAATCT CCCAGGCAAT 
TTTACTGTAA ACACACCACC TACTCCAGAA GACCTGATGT TAAGTCAATA TGTTTACCGA 
CCCAAGATAC AGATTTATAG AGAAGATTGT GAGGCTCGTC GCCAAAAGAT TGAAGAATTA 
AAGCTTTCTG CATCGAACCA AGATAAGCTG TTGGTTGATA TAAATAAGAA CCTGTGGGAA 
AAAATGAGAC ACTGCTCTGA CAAAGAGCTG AAGGCCTTTG GAATTTATCT TAACAAAATA 
AAGTCATGTT TTACCAAGAT GACTAAAGTC TTCACTCACC AAGGAAAAGT GGCTCTGTAT 
GGCAAGCTGG TGCAGTCAGC TCAGAATGAG AGGGAGAAAC TTCAAATAAA GATAGATGAG 
ATGGATAAAA TACTTAAGAA GATCGATAAC TGCCTCACTG AGATGGAAAC AGAAACTAAG 
AATTTGGAGG ATGAAGAGAA AAACAATCCT GTGGAAGAAT GGGATTCTGA AATGAGAGCT 
GCAGAAAAAG AATTGGAACA GCTGAAAACT GAAGAAGAGG AGCTTCAAAG AAATCTCTTA 
GAACTGGAGG TACAAAAAGA GCAGACCCTT GCTCAAATAG ACTTTATGCA AAAACAAAGA 
AATAGAACTG AAGAGCTACT GGATCAGTTG AGCTTGTCTG AGTGGGATGT CGTTGAGTGG 
AGTGATGATC AAGCTGTATT CACCTTTGTT TATGACACGA TACAACTCAC CATCACCTTT 
GAAGAGTCAG TTGTTGGTTT CCCTTTCCTG GACAAGCGTT ATAGGAAGAT TGTTGATGTC 
AATTTTCAAT CTCTGTTAGA TGAGGATCAA GCTCCTCCTT CCTCCCTTTT AGTTCATAAG 
CTTATTTTCC AGTACGTTGA AGAAAAGGAA TCCTGGAAGA AGACATGTAC AACCCAGCAT 
CAGTTACCCA AGATGCTTGA AGAATTCTCA CTGGTAGTGC ACCATTGCAG ACTCCTTGGA 
GAGGAGATTG AGTATTTAAA GAGATGGGGA CCAAATTATA ACCTAATGAA CATAGATATT 
AATAATAATG AATTGAGACT TTTATTCTCT AGCTCCGCAG CATTTGCAAA GTTTGAAATA 
ACTTTGTTTC TCTCAGCCTA TTATCCATCT GTACCATTAC CTTCCACCAT TCAGAATCAC 
GTTGGGAACA CTAGCCAAGA TGATATTGCT ACCATTCTAT CTAAAGTGCC ACTGGAGAAC 
AACTACCTGA AGAATGTAGT CAAGCAAATT TACCAAGATC TGTTTCAGGA CTGCCATTTC 
TACCACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KNL1 ( NM_144508.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_653091.3 Homo sapiens kinetochore scaffold 1 (KNL1), transcript variant 2, mRNA.

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CloneID	OHu21655
Clone ID Related Accession (Same CDS sequence)	NM_144508.4 , NM_144508.5
Accession Version	NM_144508.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6951bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	kinetochore scaffold 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC014306.1, AF461041.1, AC022405.5 and AB046790.1. On Jan 30, 2013 this sequence version replaced NM_144508.3. Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF461041.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END##

ATGGATGGGG TGTCTTCAGA GGCTAATGAA GAAAATGACA ATATAGAGAG ACCTGTTAGA 
AGACGGCATT CTTCAATATT GAAACCCCCA AGGAGTCCTC TTCAGGACCT CAGAGGTGGG 
AATGAAAGAG TTCAGGAATC CAATGCTTTG AGAAATAAGA AAAACTCTCG TCGAGTCAGC 
TTTGCAGATA CTATAAAGGT ATTCCAGACG GAGTCTCATA TGAAAATAGT GAGAAAGTCA 
GAAATGGAAG AAACAGAAAC AGGAGAAAAT CTTCTTTTGA TACAGAATAA GAAATTAGAA 
GATAATTACT GTGAAATTAC TGGGATGAAC ACATTGCTTT CTGCTCCCAT TCATACCCAG 
ATGCAACAGA AGGAGTTTTC AATTATAGAA CATACCCGTG AAAGGAAACA TGCAAATGAC 
CAGACAGTCA TTTTTTCAGA TGAAAACCAG ATGGACCTGA CATCAAGTCA CACTGTAATG 
ATTACCAAAG GCCTTTTAGA TAATCCCATA AGTGAAAAGT CCACCAAGAT AGATACCACA 
TCATTTCTAG CTAATTTAAA GCTTCACACC GAGGACTCAA GAATGAAAAA AGAAGTAAAT 
TTTTCCGTGG ATCAAAACAC TTCTTCAGAA AATAAAATAG ATTTCAATGA CTTCATAAAA 
AGATTGAAAA CAGGAAAATG TAGTGCTTTT CCTGATGTGC CTGATAAAGA AAATTTTGAG 
ATACCTATTT ATTCCAAGGA ACCGAACAGT GCCTCTTCTA CACATCAAAT GCATGTATCT 
CTTAAGGAAG ATGAAAATAA CAGTAATATT ACTAGGCTCT TTAGAGAAAA AGATGATGGG 
ATGAATTTCA CCCAGTGTCA TACAGCCAAT ATTCAGACAT TGATTCCCAC ATCCAGTGAG 
ACCAACTCAC GGGAATCTAA AGGTAATGAT ATTACAATTT ATGGCAATGA CTTTATGGAC 
TTGACATTTA ACCACACTTT GCAGATCTTA CCTGCAACAG GTAATTTTTC TGAAATAGAA 
AATCAAACTC AGAATGCCAT GGATGTAACA ACAGGTTATG GAACTAAAGC TTCAGGAAAT 
AAAACAGTTT TTAAGAGTAA ACAAAATACT GCTTTTCAAG ACCTTTCCAT AAACTCTGCA 
GACAAAATAC ATATTACCAG AAGTCATATT ATGGGGGCAG AAACTCACAT AGTCTCACAG 
ACTTGTAATC AGGATGCCAG AATATTAGCC ATGACCCCAG AATCTATATA TTCTAATCCA 
TCTATTCAAG GTTGTAAGAC TGTTTTCTAT TCTAGTTGTA ATGATGCCAT GGAAATGACC 
AAATGTCTCT CAAATATGAG AGAGGAGAAA AATTTGCTAA AGCATGACAG TAATTATGCT 
AAAATGTATT GCAATCCAGA TGCTATGTCT TCTCTCACAG AGAAAACTAT TTATTCCGGA 
GAGGAGAACA TGGACATTAC CAAGAGTCAT ACAGTTGCAA TAGATAATCA AATTTTTAAA 
CAAGATCAAT CAAATGTGCA AATAGCAGCT GCACCAACAC CCGAAAAAGA AATGATGCTC 
CAAAATCTTA TGACCACATC AGAAGATGGG AAAATGAATG TAAATTGTAA CTCAGTTCCT 
CATGTATCTA AGGAAAGAAT ACAGCAGAGC CTGTCAAATC CTTTGTCTAT TTCATTGACT 
GATAGAAAGA CTGAACTCTT ATCAGGTGAA AATATGGATT TGACTGAAAG TCACACAAGT 
AACTTAGGAA GTCAGGTTCC TCTTGCAGCT TATAATCTAG CACCGGAGAG TACCAGTGAA 
TCTCACTCTC AGAGCAAAAG CTCTTCAGAT GAATGTGAAG AAATTACCAA AAGTCGTAAT 
GAACCATTTC AGCGATCAGA CATAATAGCC AAAAACAGCT TAACCGACAC CTGGAACAAA 
GACAAAGATT GGGTTTTGAA GATTTTGCCC TACCTTGATA AAGATTCTCC TCAGTCAGCT 
GATTGTAATC AGGAGATAGC AACAAGCCAT AATATAGTCT ACTGTGGTGG AGTTCTTGAT 
AAACAAATAA CTAATAGAAA TACAGTATCA TGGGAACAAT CTTTGTTTTC TACCACAAAG 
CCATTATTTT CATCAGGACA GTTCTCTATG AAAAATCATG ATACTGCTAT AAGTAGTCAT 
ACAGTGAAAT CTGTACTAGG CCAGAATTCT AAACTGGCTG AGCCACTGAG GAAAAGTTTA 
AGCAATCCCA CACCTGACTA TTGCCATGAC AAGATGATTA TATGTTCAGA GGAAGAGCAA 
AATATGGATC TAACAAAGAG CCACACTGTC GTCATTGGAT TTGGTCCTTC TGAACTACAA 
GAACTTGGTA AAACTAATTT AGAACACACT ACTGGCCAGC TAACAACAAT GAACAGACAG 
ATAGCTGTAA AAGTTGAAAA ATGTGGTAAA AGTCCCATAG AAAAAAGTGG AGTGCTTAAA 
TCTAACTGTA TTATGGATGT GTTAGAGGAC GAAAGTGTAC AGAAACCTAA ATTTCCAAAG 
GAAAAGCAAA ATGTCAAAAT TTGGGGAAGG AAAAGTGTTG GTGGACCAAA AATTGATAAG 
ACTATTGTAT TTTCAGAAGA CGATAAGAAT GATATGGATA TCACTAAGAG TTATACAATA 
GAAATAAACC ATAGACCTTT ATTAGAGAAA CGTGATTGTC ATTTGGTGCC ATTGGCAGGA 
ACTTCTGAAA CTATTTTATA TACATGTAGG CAGGATGACA TGGAGATCAC TAGAAGTCAC 
ACAACTGCCT TAGAATGTAA AACTGTCTCA CCAGATGAAA TAACTACTAG GCCTATGGAC 
AAAACTGTAG TGTTTGTAGA TAATCATGTT GAACTAGAAA TGACAGAGTC CCATACTGTT 
TTCATTGACT ACCAAGAAAA GGAAAGAACA GACAGACCTA ACTTTGAACT ATCCCAAAGG 
AAAAGCCTAG GAACACCAAC AGTGATATGT ACTCCTACTG AGGAGAGTGT TTTCTTTCCA 
GGAAATGGTG AAAGTGACCG TCTAGTAGCA AATGACAGCC AGCTAACCCC TCTGGAGGAA 
TGGTCTAATA ATAGGGGCCC TGTAGAGGTA GCTGATAACA TGGAATTGTC TAAATCAGCC 
ACTTGCAAAA ACATCAAAGA TGTACAAAGT CCTGGATTTC TGAATGAACC TCTATCAAGC 
AAAAGTCAGA GAAGAAAAAG CCTTAAGCTA AAAAATGACA AGACCATTGT ATTTTCAGAG 
AATCATAAAA ATGATATGGA TATTACCCAG AGTTGTATGG TGGAAATAGA TAACGAAAGT 
GCCCTGGAGG ATAAAGAGGA CTTCCATTTG GCAGGGGCTT CTAAAACTAT TTTGTATTCA 
TGTGGGCAGG ATGACATGGA GATCACTAGG AGTCACACAA CTGCCTTAGA ATGTAAAACT 
CTCCTGCCAA ATGAAATAGC TATTAGGCCC ATGGACAAAA CCGTATTGTT CACAGATAAT 
TACAGTGATC TGGAAGTCAC CGATTCCCAT ACTGTTTTCA TTGACTGTCA AGCCACAGAG 
AAAATACTTG AAGAAAACCC TAAATTTGGA ATAGGAAAAG GAAAAAACTT GGGTGTTTCC 
TTTCCTAAGG ATAATAGCTG TGTTCAAGAA ATCGCTGAAA AACAAGCACT GGCTGTAGGA 
AACAAAATAG TTCTTCACAC CGAGCAAAAG CAACAACTCT TTGCTGCTAC TAATAGAACT 
ACTAATGAAA TCATCAAATT TCATAGTGCT GCTATGGATG AAAAGGTCAT AGGGAAAGTT 
GTAGACCAGG CCTGTACATT GGAAAAAGCG CAAGTTGAAA GCTGTCAGTT AAATAATAGA 
GATAGAAGAA ATGTGGACTT TACAAGTAGT CATGCAACTG CTGTTTGTGG ATCCAGTGAT 
AATTATTCCT GTTTACCAAA TGTTATTTCC TGTACTGATA ATTTGGAGGG TAGTGCCATG 
CTCTTATGTG ATAAAGATGA GGAAAAAGCC AATTATTGCC CAGTGCAAAA TGATCTTGCT 
TATGCAAATG ATTTTGCCAG TGAATATTAC TTGGAATCTG AGGGACAGCC TCTCTCTGCT 
CCTTGTCCTT TGTTAGAGAA GGAAGAAGTT ATTCAAACCA GTACCAAAGG ACAGTTAGAC 
TGTGTTATAA CACTGCACAA AGATCAAGAT CTGATTAAGG ATCCACGAAA TCTATTGGCT 
AATCAAACTT TAGTATATAG TCAAGATCTG GGGGAGATGA CTAAACTTAA TTCAAAGCGA 
GTATCTTTTA AGCTTCCAAA GGATCAAATG AAAGTCTATG TTGATGACAT TTATGTTATT 
CCTCAGCCTC ATTTCTCAAC CGACCAACCT CCATTACCTA AAAAAGGACA GAGTAGTATC 
AATAAAGAAG AAGTAATACT GTCTAAAGCT GGAAATAAGA GTTTAAATAT TATAGAAAAT 
TCCTCTGCAC CCATATGTGA AAACAAGCCC AAAATACTCA ATAGTGAGGA ATGGTTTGCT 
GCAGCCTGTA AAAAAGAACT GAAGGAAAAT ATTCAAACAA CTAACTATAA TACAGCTCTA 
GATTTCCACA GTAACTCAGA CGTAACTAAG CAAGTCATTC AAACTCATGT CAATGCTGGA 
GAAGCACCAG ATCCTGTAAT TACATCTAAT GTTCCATGTT TTCATAGTAT CAAACCAAAT 
CTGAATAATT TGAATGGAAA AACTGGAGAG TTTTTAGCCT TTCAAACTGT TCATCTACCA 
CCCCTTCCAG AGCAATTACT TGAATTAGGA AATAAGGCAC ACAATGATAT GCATATAGTG 
CAAGCTACAG AAATACATAA TATTAACATA ATCTCCAGCA ATGCTAAAGA TAGTAGAGAT 
GAGGAAAATA AAAAGTCTCA TAATGGAGCT GAAACCACCT CTCTACCGCC AAAGACAGTT 
TTTAAAGATA AAGTAAGGAG ATGTTCTTTG GGAATCTTTT TGCCTAGATT GCCCAACAAG 
AGAAATTGTA GTGTCACTGG TATTGATGAC CTGGAACAGA TTCCAGCAGA CACAACTGAT 
ATAAATCACT TAGAAACTCA GCCGGTCTCT AGCAAAGATT CAGGCATTGG ATCTGTTGCA 
GGTAAACTGA ACCTAAGTCC TTCTCAATAT ATAAATGAGG AAAATCTTCC TGTATATCCT 
GATGAGATCA ATTCTTCAGA CTCTATTAAC ATAGAAACTG AGGAAAAGGC CTTGATTGAG 
ACATACCAAA AAGAGATTTC ACCATATGAA AATAAAATGG GAAAAACTTG CAATAGCCAA 
AAAAGAACGT GGGTACAAGA AGAAGAAGAT ATTCATAAGG AGAAAAAAAT CAGAAAAAAT 
GAGATTAAGT TTAGTGATAC GACACAAGAT CGGGAGATTT TTGATCACCA TACTGAAGAG 
GATATAGATA AAAGTGCTAA CAGTGTATTG ATAAAAAACC TGAGCAGGAC CCCATCTAGT 
TGCAGCAGCT CTCTGGATTC AATCAAGGCT GATGGGACCT CTCTGGACTT CAGCACTTAC 
CGCAGTAGTC AAATGGAATC ACAGTTTCTC AGAGATACTA TTTGTGAAGA GAGCTTGAGG 
GAGAAACTCC AAGATGGGAG AATAACAATA AGGGAGTTCT TTATACTTCT CCAGGTCCAC 
ATCTTGATAC AGAAACCCCG ACAGAGCAAT CTCCCAGGCA ATTTTACTGT AAACACACCA 
CCTACTCCAG AAGACCTGAT GTTAAGTCAA TATGTTTACC GACCCAAGAT ACAGATTTAT 
AGAGAAGATT GTGAGGCTCG TCGCCAAAAG ATTGAAGAAT TAAAGCTTTC TGCATCGAAC 
CAAGATAAGC TGTTGGTTGA TATAAATAAG AACCTGTGGG AAAAAATGAG ACACTGCTCT 
GACAAAGAGC TGAAGGCCTT TGGAATTTAT CTTAACAAAA TAAAGTCATG TTTTACCAAG 
ATGACTAAAG TCTTCACTCA CCAAGGAAAA GTGGCTCTGT ATGGCAAGCT GGTGCAGTCA 
GCTCAGAATG AGAGGGAGAA ACTTCAAATA AAGATAGATG AGATGGATAA AATACTTAAG 
AAGATCGATA ACTGCCTCAC TGAGATGGAA ACAGAAACTA AGAATTTGGA GGATGAAGAG 
AAAAACAATC CTGTGGAAGA ATGGGATTCT GAAATGAGAG CTGCAGAAAA AGAATTGGAA 
CAGCTGAAAA CTGAAGAAGA GGAGCTTCAA AGAAATCTCT TAGAACTGGA GGTACAAAAA 
GAGCAGACCC TTGCTCAAAT AGACTTTATG CAAAAACAAA GAAATAGAAC TGAAGAGCTA 
CTGGATCAGT TGAGCTTGTC TGAGTGGGAT GTCGTTGAGT GGAGTGATGA TCAAGCTGTA 
TTCACCTTTG TTTATGACAC GATACAACTC ACCATCACCT TTGAAGAGTC AGTTGTTGGT 
TTCCCTTTCC TGGACAAGCG TTATAGGAAG ATTGTTGATG TCAATTTTCA ATCTCTGTTA 
GATGAGGATC AAGCTCCTCC TTCCTCCCTT TTAGTTCATA AGCTTATTTT CCAGTACGTT 
GAAGAAAAGG AATCCTGGAA GAAGACATGT ACAACCCAGC ATCAGTTACC CAAGATGCTT 
GAAGAATTCT CACTGGTAGT GCACCATTGC AGACTCCTTG GAGAGGAGAT TGAGTATTTA 
AAGAGATGGG GACCAAATTA TAACCTAATG AACATAGATA TTAATAATAA TGAATTGAGA 
CTTTTATTCT CTAGCTCCGC AGCATTTGCA AAGTTTGAAA TAACTTTGTT TCTCTCAGCC 
TATTATCCAT CTGTACCATT ACCTTCCACC ATTCAGAATC ACGTTGGGAA CACTAGCCAA 
GATGATATTG CTACCATTCT ATCTAAAGTG CCACTGGAGA ACAACTACCT GAAGAATGTA 
GTCAAGCAAA TTTACCAAGA TCTGTTTCAG GACTGCCATT TCTACCACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_653091.3
CDS	162..7112
Translation	MDGVSSEANEENDNIERPVRRRHSSILKPPRSPLQDLRGGNERVQESNALRNKKNSRRVSFADTIKVFQTESHMKIVRKSEMEETETGENLLLIQNKKLEDNYCEITGMNTLLSAPIHTQMQQKEFSIIEHTRERKHANDQTVIFSDENQMDLTSSHTVMITKGLLDNPISEKSTKIDTTSFLANLKLHTEDSRMKKEVNFSVDQNTSSENKIDFNDFIKRLKTGKCSAFPDVPDKENFEIPIYSKEPNSASSTHQMHVSLKEDENNSNITRLFREKDDGMNFTQCHTANIQTLIPTSSETNSRESKGNDITIYGNDFMDLTFNHTLQILPATGNFSEIENQTQNAMDVTTGYGTKASGNKTVFKSKQNTAFQDLSINSADKIHITRSHIMGAETHIVSQTCNQDARILAMTPESIYSNPSIQGCKTVFYSSCNDAMEMTKCLSNMREEKNLLKHDSNYAKMYCNPDAMSSLTEKTIYSGEENMDITKSHTVAIDNQIFKQDQSNVQIAAAPTPEKEMMLQNLMTTSEDGKMNVNCNSVPHVSKERIQQSLSNPLSISLTDRKTELLSGENMDLTESHTSNLGSQVPLAAYNLAPESTSESHSQSKSSSDECEEITKSRNEPFQRSDIIAKNSLTDTWNKDKDWVLKILPYLDKDSPQSADCNQEIATSHNIVYCGGVLDKQITNRNTVSWEQSLFSTTKPLFSSGQFSMKNHDTAISSHTVKSVLGQNSKLAEPLRKSLSNPTPDYCHDKMIICSEEEQNMDLTKSHTVVIGFGPSELQELGKTNLEHTTGQLTTMNRQIAVKVEKCGKSPIEKSGVLKSNCIMDVLEDESVQKPKFPKEKQNVKIWGRKSVGGPKIDKTIVFSEDDKNDMDITKSYTIEINHRPLLEKRDCHLVPLAGTSETILYTCRQDDMEITRSHTTALECKTVSPDEITTRPMDKTVVFVDNHVELEMTESHTVFIDYQEKERTDRPNFELSQRKSLGTPTVICTPTEESVFFPGNGESDRLVANDSQLTPLEEWSNNRGPVEVADNMELSKSATCKNIKDVQSPGFLNEPLSSKSQRRKSLKLKNDKTIVFSENHKNDMDITQSCMVEIDNESALEDKEDFHLAGASKTILYSCGQDDMEITRSHTTALECKTLLPNEIAIRPMDKTVLFTDNYSDLEVTDSHTVFIDCQATEKILEENPKFGIGKGKNLGVSFPKDNSCVQEIAEKQALAVGNKIVLHTEQKQQLFAATNRTTNEIIKFHSAAMDEKVIGKVVDQACTLEKAQVESCQLNNRDRRNVDFTSSHATAVCGSSDNYSCLPNVISCTDNLEGSAMLLCDKDEEKANYCPVQNDLAYANDFASEYYLESEGQPLSAPCPLLEKEEVIQTSTKGQLDCVITLHKDQDLIKDPRNLLANQTLVYSQDLGEMTKLNSKRVSFKLPKDQMKVYVDDIYVIPQPHFSTDQPPLPKKGQSSINKEEVILSKAGNKSLNIIENSSAPICENKPKILNSEEWFAAACKKELKENIQTTNYNTALDFHSNSDVTKQVIQTHVNAGEAPDPVITSNVPCFHSIKPNLNNLNGKTGEFLAFQTVHLPPLPEQLLELGNKAHNDMHIVQATEIHNINIISSNAKDSRDEENKKSHNGAETTSLPPKTVFKDKVRRCSLGIFLPRLPNKRNCSVTGIDDLEQIPADTTDINHLETQPVSSKDSGIGSVAGKLNLSPSQYINEENLPVYPDEINSSDSINIETEEKALIETYQKEISPYENKMGKTCNSQKRTWVQEEEDIHKEKKIRKNEIKFSDTTQDREIFDHHTEEDIDKSANSVLIKNLSRTPSSCSSSLDSIKADGTSLDFSTYRSSQMESQFLRDTICEESLREKLQDGRITIREFFILLQVHILIQKPRQSNLPGNFTVNTPPTPEDLMLSQYVYRPKIQIYREDCEARRQKIEELKLSASNQDKLLVDINKNLWEKMRHCSDKELKAFGIYLNKIKSCFTKMTKVFTHQGKVALYGKLVQSAQNEREKLQIKIDEMDKILKKIDNCLTEMETETKNLEDEEKNNPVEEWDSEMRAAEKELEQLKTEEEELQRNLLELEVQKEQTLAQIDFMQKQRNRTEELLDQLSLSEWDVVEWSDDQAVFTFVYDTIQLTITFEESVVGFPFLDKRYRKIVDVNFQSLLDEDQAPPSSLLVHKLIFQYVEEKESWKKTCTTQHQLPKMLEEFSLVVHHCRLLGEEIEYLKRWGPNYNLMNIDINNNELRLLFSSSAAFAKFEITLFLSAYYPSVPLPSTIQNHVGNTSQDDIATILSKVPLENNYLKNVVKQIYQDLFQDCHFYH

Target ORF information:

RefSeq Version	NM_144508.4
Organism	Homo sapiens(human)
Definition	Homo sapiens kinetochore scaffold 1 (KNL1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_144508.4

ORF Insert Sequence:

ATGGATGGGG TGTCTTCAGA GGCTAATGAA GAAAATGACA ATATAGAGAG ACCTGTTAGA 
AGACGGCATT CTTCAATATT GAAACCCCCA AGGAGTCCTC TTCAGGACCT CAGAGGTGGG 
AATGAAAGAG TTCAGGAATC CAATGCTTTG AGAAATAAGA AAAACTCTCG TCGAGTCAGC 
TTTGCAGATA CTATAAAGGT ATTCCAGACG GAGTCTCATA TGAAAATAGT GAGAAAGTCA 
GAAATGGAAG AAACAGAAAC AGGAGAAAAT CTTCTTTTGA TACAGAATAA GAAATTAGAA 
GATAATTACT GTGAAATTAC TGGGATGAAC ACATTGCTTT CTGCTCCCAT TCATACCCAG 
ATGCAACAGA AGGAGTTTTC AATTATAGAA CATACCCGTG AAAGGAAACA TGCAAATGAC 
CAGACAGTCA TTTTTTCAGA TGAAAACCAG ATGGACCTGA CATCAAGTCA CACTGTAATG 
ATTACCAAAG GCCTTTTAGA TAATCCCATA AGTGAAAAGT CCACCAAGAT AGATACCACA 
TCATTTCTAG CTAATTTAAA GCTTCACACC GAGGACTCAA GAATGAAAAA AGAAGTAAAT 
TTTTCCGTGG ATCAAAACAC TTCTTCAGAA AATAAAATAG ATTTCAATGA CTTCATAAAA 
AGATTGAAAA CAGGAAAATG TAGTGCTTTT CCTGATGTGC CTGATAAAGA AAATTTTGAG 
ATACCTATTT ATTCCAAGGA ACCGAACAGT GCCTCTTCTA CACATCAAAT GCATGTATCT 
CTTAAGGAAG ATGAAAATAA CAGTAATATT ACTAGGCTCT TTAGAGAAAA AGATGATGGG 
ATGAATTTCA CCCAGTGTCA TACAGCCAAT ATTCAGACAT TGATTCCCAC ATCCAGTGAG 
ACCAACTCAC GGGAATCTAA AGGTAATGAT ATTACAATTT ATGGCAATGA CTTTATGGAC 
TTGACATTTA ACCACACTTT GCAGATCTTA CCTGCAACAG GTAATTTTTC TGAAATAGAA 
AATCAAACTC AGAATGCCAT GGATGTAACA ACAGGTTATG GAACTAAAGC TTCAGGAAAT 
AAAACAGTTT TTAAGAGTAA ACAAAATACT GCTTTTCAAG ACCTTTCCAT AAACTCTGCA 
GACAAAATAC ATATTACCAG AAGTCATATT ATGGGGGCAG AAACTCACAT AGTCTCACAG 
ACTTGTAATC AGGATGCCAG AATATTAGCC ATGACCCCAG AATCTATATA TTCTAATCCA 
TCTATTCAAG GTTGTAAGAC TGTTTTCTAT TCTAGTTGTA ATGATGCCAT GGAAATGACC 
AAATGTCTCT CAAATATGAG AGAGGAGAAA AATTTGCTAA AGCATGACAG TAATTATGCT 
AAAATGTATT GCAATCCAGA TGCTATGTCT TCTCTCACAG AGAAAACTAT TTATTCCGGA 
GAGGAGAACA TGGACATTAC CAAGAGTCAT ACAGTTGCAA TAGATAATCA AATTTTTAAA 
CAAGATCAAT CAAATGTGCA AATAGCAGCT GCACCAACAC CCGAAAAAGA AATGATGCTC 
CAAAATCTTA TGACCACATC AGAAGATGGG AAAATGAATG TAAATTGTAA CTCAGTTCCT 
CATGTATCTA AGGAAAGAAT ACAGCAGAGC CTGTCAAATC CTTTGTCTAT TTCATTGACT 
GATAGAAAGA CTGAACTCTT ATCAGGTGAA AATATGGATT TGACTGAAAG TCACACAAGT 
AACTTAGGAA GTCAGGTTCC TCTTGCAGCT TATAATCTAG CACCGGAGAG TACCAGTGAA 
TCTCACTCTC AGAGCAAAAG CTCTTCAGAT GAATGTGAAG AAATTACCAA AAGTCGTAAT 
GAACCATTTC AGCGATCAGA CATAATAGCC AAAAACAGCT TAACCGACAC CTGGAACAAA 
GACAAAGATT GGGTTTTGAA GATTTTGCCC TACCTTGATA AAGATTCTCC TCAGTCAGCT 
GATTGTAATC AGGAGATAGC AACAAGCCAT AATATAGTCT ACTGTGGTGG AGTTCTTGAT 
AAACAAATAA CTAATAGAAA TACAGTATCA TGGGAACAAT CTTTGTTTTC TACCACAAAG 
CCATTATTTT CATCAGGACA GTTCTCTATG AAAAATCATG ATACTGCTAT AAGTAGTCAT 
ACAGTGAAAT CTGTACTAGG CCAGAATTCT AAACTGGCTG AGCCACTGAG GAAAAGTTTA 
AGCAATCCCA CACCTGACTA TTGCCATGAC AAGATGATTA TATGTTCAGA GGAAGAGCAA 
AATATGGATC TAACAAAGAG CCACACTGTC GTCATTGGAT TTGGTCCTTC TGAACTACAA 
GAACTTGGTA AAACTAATTT AGAACACACT ACTGGCCAGC TAACAACAAT GAACAGACAG 
ATAGCTGTAA AAGTTGAAAA ATGTGGTAAA AGTCCCATAG AAAAAAGTGG AGTGCTTAAA 
TCTAACTGTA TTATGGATGT GTTAGAGGAC GAAAGTGTAC AGAAACCTAA ATTTCCAAAG 
GAAAAGCAAA ATGTCAAAAT TTGGGGAAGG AAAAGTGTTG GTGGACCAAA AATTGATAAG 
ACTATTGTAT TTTCAGAAGA CGATAAGAAT GATATGGATA TCACTAAGAG TTATACAATA 
GAAATAAACC ATAGACCTTT ATTAGAGAAA CGTGATTGTC ATTTGGTGCC ATTGGCAGGA 
ACTTCTGAAA CTATTTTATA TACATGTAGG CAGGATGACA TGGAGATCAC TAGAAGTCAC 
ACAACTGCCT TAGAATGTAA AACTGTCTCA CCAGATGAAA TAACTACTAG GCCTATGGAC 
AAAACTGTAG TGTTTGTAGA TAATCATGTT GAACTAGAAA TGACAGAGTC CCATACTGTT 
TTCATTGACT ACCAAGAAAA GGAAAGAACA GACAGACCTA ACTTTGAACT ATCCCAAAGG 
AAAAGCCTAG GAACACCAAC AGTGATATGT ACTCCTACTG AGGAGAGTGT TTTCTTTCCA 
GGAAATGGTG AAAGTGACCG TCTAGTAGCA AATGACAGCC AGCTAACCCC TCTGGAGGAA 
TGGTCTAATA ATAGGGGCCC TGTAGAGGTA GCTGATAACA TGGAATTGTC TAAATCAGCC 
ACTTGCAAAA ACATCAAAGA TGTACAAAGT CCTGGATTTC TGAATGAACC TCTATCAAGC 
AAAAGTCAGA GAAGAAAAAG CCTTAAGCTA AAAAATGACA AGACCATTGT ATTTTCAGAG 
AATCATAAAA ATGATATGGA TATTACCCAG AGTTGTATGG TGGAAATAGA TAACGAAAGT 
GCCCTGGAGG ATAAAGAGGA CTTCCATTTG GCAGGGGCTT CTAAAACTAT TTTGTATTCA 
TGTGGGCAGG ATGACATGGA GATCACTAGG AGTCACACAA CTGCCTTAGA ATGTAAAACT 
CTCCTGCCAA ATGAAATAGC TATTAGGCCC ATGGACAAAA CCGTATTGTT CACAGATAAT 
TACAGTGATC TGGAAGTCAC CGATTCCCAT ACTGTTTTCA TTGACTGTCA AGCCACAGAG 
AAAATACTTG AAGAAAACCC TAAATTTGGA ATAGGAAAAG GAAAAAACTT GGGTGTTTCC 
TTTCCTAAGG ATAATAGCTG TGTTCAAGAA ATCGCTGAAA AACAAGCACT GGCTGTAGGA 
AACAAAATAG TTCTTCACAC CGAGCAAAAG CAACAACTCT TTGCTGCTAC TAATAGAACT 
ACTAATGAAA TCATCAAATT TCATAGTGCT GCTATGGATG AAAAGGTCAT AGGGAAAGTT 
GTAGACCAGG CCTGTACATT GGAAAAAGCG CAAGTTGAAA GCTGTCAGTT AAATAATAGA 
GATAGAAGAA ATGTGGACTT TACAAGTAGT CATGCAACTG CTGTTTGTGG ATCCAGTGAT 
AATTATTCCT GTTTACCAAA TGTTATTTCC TGTACTGATA ATTTGGAGGG TAGTGCCATG 
CTCTTATGTG ATAAAGATGA GGAAAAAGCC AATTATTGCC CAGTGCAAAA TGATCTTGCT 
TATGCAAATG ATTTTGCCAG TGAATATTAC TTGGAATCTG AGGGACAGCC TCTCTCTGCT 
CCTTGTCCTT TGTTAGAGAA GGAAGAAGTT ATTCAAACCA GTACCAAAGG ACAGTTAGAC 
TGTGTTATAA CACTGCACAA AGATCAAGAT CTGATTAAGG ATCCACGAAA TCTATTGGCT 
AATCAAACTT TAGTATATAG TCAAGATCTG GGGGAGATGA CTAAACTTAA TTCAAAGCGA 
GTATCTTTTA AGCTTCCAAA GGATCAAATG AAAGTCTATG TTGATGACAT TTATGTTATT 
CCTCAGCCTC ATTTCTCAAC CGACCAACCT CCATTACCTA AAAAAGGACA GAGTAGTATC 
AATAAAGAAG AAGTAATACT GTCTAAAGCT GGAAATAAGA GTTTAAATAT TATAGAAAAT 
TCCTCTGCAC CCATATGTGA AAACAAGCCC AAAATACTCA ATAGTGAGGA ATGGTTTGCT 
GCAGCCTGTA AAAAAGAACT GAAGGAAAAT ATTCAAACAA CTAACTATAA TACAGCTCTA 
GATTTCCACA GTAACTCAGA CGTAACTAAG CAAGTCATTC AAACTCATGT CAATGCTGGA 
GAAGCACCAG ATCCTGTAAT TACATCTAAT GTTCCATGTT TTCATAGTAT CAAACCAAAT 
CTGAATAATT TGAATGGAAA AACTGGAGAG TTTTTAGCCT TTCAAACTGT TCATCTACCA 
CCCCTTCCAG AGCAATTACT TGAATTAGGA AATAAGGCAC ACAATGATAT GCATATAGTG 
CAAGCTACAG AAATACATAA TATTAACATA ATCTCCAGCA ATGCTAAAGA TAGTAGAGAT 
GAGGAAAATA AAAAGTCTCA TAATGGAGCT GAAACCACCT CTCTACCGCC AAAGACAGTT 
TTTAAAGATA AAGTAAGGAG ATGTTCTTTG GGAATCTTTT TGCCTAGATT GCCCAACAAG 
AGAAATTGTA GTGTCACTGG TATTGATGAC CTGGAACAGA TTCCAGCAGA CACAACTGAT 
ATAAATCACT TAGAAACTCA GCCGGTCTCT AGCAAAGATT CAGGCATTGG ATCTGTTGCA 
GGTAAACTGA ACCTAAGTCC TTCTCAATAT ATAAATGAGG AAAATCTTCC TGTATATCCT 
GATGAGATCA ATTCTTCAGA CTCTATTAAC ATAGAAACTG AGGAAAAGGC CTTGATTGAG 
ACATACCAAA AAGAGATTTC ACCATATGAA AATAAAATGG GAAAAACTTG CAATAGCCAA 
AAAAGAACGT GGGTACAAGA AGAAGAAGAT ATTCATAAGG AGAAAAAAAT CAGAAAAAAT 
GAGATTAAGT TTAGTGATAC GACACAAGAT CGGGAGATTT TTGATCACCA TACTGAAGAG 
GATATAGATA AAAGTGCTAA CAGTGTATTG ATAAAAAACC TGAGCAGGAC CCCATCTAGT 
TGCAGCAGCT CTCTGGATTC AATCAAGGCT GATGGGACCT CTCTGGACTT CAGCACTTAC 
CGCAGTAGTC AAATGGAATC ACAGTTTCTC AGAGATACTA TTTGTGAAGA GAGCTTGAGG 
GAGAAACTCC AAGATGGGAG AATAACAATA AGGGAGTTCT TTATACTTCT CCAGGTCCAC 
ATCTTGATAC AGAAACCCCG ACAGAGCAAT CTCCCAGGCA ATTTTACTGT AAACACACCA 
CCTACTCCAG AAGACCTGAT GTTAAGTCAA TATGTTTACC GACCCAAGAT ACAGATTTAT 
AGAGAAGATT GTGAGGCTCG TCGCCAAAAG ATTGAAGAAT TAAAGCTTTC TGCATCGAAC 
CAAGATAAGC TGTTGGTTGA TATAAATAAG AACCTGTGGG AAAAAATGAG ACACTGCTCT 
GACAAAGAGC TGAAGGCCTT TGGAATTTAT CTTAACAAAA TAAAGTCATG TTTTACCAAG 
ATGACTAAAG TCTTCACTCA CCAAGGAAAA GTGGCTCTGT ATGGCAAGCT GGTGCAGTCA 
GCTCAGAATG AGAGGGAGAA ACTTCAAATA AAGATAGATG AGATGGATAA AATACTTAAG 
AAGATCGATA ACTGCCTCAC TGAGATGGAA ACAGAAACTA AGAATTTGGA GGATGAAGAG 
AAAAACAATC CTGTGGAAGA ATGGGATTCT GAAATGAGAG CTGCAGAAAA AGAATTGGAA 
CAGCTGAAAA CTGAAGAAGA GGAGCTTCAA AGAAATCTCT TAGAACTGGA GGTACAAAAA 
GAGCAGACCC TTGCTCAAAT AGACTTTATG CAAAAACAAA GAAATAGAAC TGAAGAGCTA 
CTGGATCAGT TGAGCTTGTC TGAGTGGGAT GTCGTTGAGT GGAGTGATGA TCAAGCTGTA 
TTCACCTTTG TTTATGACAC GATACAACTC ACCATCACCT TTGAAGAGTC AGTTGTTGGT 
TTCCCTTTCC TGGACAAGCG TTATAGGAAG ATTGTTGATG TCAATTTTCA ATCTCTGTTA 
GATGAGGATC AAGCTCCTCC TTCCTCCCTT TTAGTTCATA AGCTTATTTT CCAGTACGTT 
GAAGAAAAGG AATCCTGGAA GAAGACATGT ACAACCCAGC ATCAGTTACC CAAGATGCTT 
GAAGAATTCT CACTGGTAGT GCACCATTGC AGACTCCTTG GAGAGGAGAT TGAGTATTTA 
AAGAGATGGG GACCAAATTA TAACCTAATG AACATAGATA TTAATAATAA TGAATTGAGA 
CTTTTATTCT CTAGCTCCGC AGCATTTGCA AAGTTTGAAA TAACTTTGTT TCTCTCAGCC 
TATTATCCAT CTGTACCATT ACCTTCCACC ATTCAGAATC ACGTTGGGAA CACTAGCCAA 
GATGATATTG CTACCATTCT ATCTAAAGTG CCACTGGAGA ACAACTACCT GAAGAATGTA 
GTCAAGCAAA TTTACCAAGA TCTGTTTCAG GACTGCCATT TCTACCACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KNL1 ( NM_144508.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_653091.3 Homo sapiens kinetochore scaffold 1 (KNL1), transcript variant 2, mRNA.

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CloneID	OHu21655
Clone ID Related Accession (Same CDS sequence)	NM_144508.4 , NM_144508.5
Accession Version	NM_144508.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6951bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	kinetochore scaffold 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC014306.1, AF461041.1, AC022405.5 and AB046790.1. On Jun 2, 2019 this sequence version replaced NM_144508.4. Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF461041.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END##

ATGGATGGGG TGTCTTCAGA GGCTAATGAA GAAAATGACA ATATAGAGAG ACCTGTTAGA 
AGACGGCATT CTTCAATATT GAAACCCCCA AGGAGTCCTC TTCAGGACCT CAGAGGTGGG 
AATGAAAGAG TTCAGGAATC CAATGCTTTG AGAAATAAGA AAAACTCTCG TCGAGTCAGC 
TTTGCAGATA CTATAAAGGT ATTCCAGACG GAGTCTCATA TGAAAATAGT GAGAAAGTCA 
GAAATGGAAG AAACAGAAAC AGGAGAAAAT CTTCTTTTGA TACAGAATAA GAAATTAGAA 
GATAATTACT GTGAAATTAC TGGGATGAAC ACATTGCTTT CTGCTCCCAT TCATACCCAG 
ATGCAACAGA AGGAGTTTTC AATTATAGAA CATACCCGTG AAAGGAAACA TGCAAATGAC 
CAGACAGTCA TTTTTTCAGA TGAAAACCAG ATGGACCTGA CATCAAGTCA CACTGTAATG 
ATTACCAAAG GCCTTTTAGA TAATCCCATA AGTGAAAAGT CCACCAAGAT AGATACCACA 
TCATTTCTAG CTAATTTAAA GCTTCACACC GAGGACTCAA GAATGAAAAA AGAAGTAAAT 
TTTTCCGTGG ATCAAAACAC TTCTTCAGAA AATAAAATAG ATTTCAATGA CTTCATAAAA 
AGATTGAAAA CAGGAAAATG TAGTGCTTTT CCTGATGTGC CTGATAAAGA AAATTTTGAG 
ATACCTATTT ATTCCAAGGA ACCGAACAGT GCCTCTTCTA CACATCAAAT GCATGTATCT 
CTTAAGGAAG ATGAAAATAA CAGTAATATT ACTAGGCTCT TTAGAGAAAA AGATGATGGG 
ATGAATTTCA CCCAGTGTCA TACAGCCAAT ATTCAGACAT TGATTCCCAC ATCCAGTGAG 
ACCAACTCAC GGGAATCTAA AGGTAATGAT ATTACAATTT ATGGCAATGA CTTTATGGAC 
TTGACATTTA ACCACACTTT GCAGATCTTA CCTGCAACAG GTAATTTTTC TGAAATAGAA 
AATCAAACTC AGAATGCCAT GGATGTAACA ACAGGTTATG GAACTAAAGC TTCAGGAAAT 
AAAACAGTTT TTAAGAGTAA ACAAAATACT GCTTTTCAAG ACCTTTCCAT AAACTCTGCA 
GACAAAATAC ATATTACCAG AAGTCATATT ATGGGGGCAG AAACTCACAT AGTCTCACAG 
ACTTGTAATC AGGATGCCAG AATATTAGCC ATGACCCCAG AATCTATATA TTCTAATCCA 
TCTATTCAAG GTTGTAAGAC TGTTTTCTAT TCTAGTTGTA ATGATGCCAT GGAAATGACC 
AAATGTCTCT CAAATATGAG AGAGGAGAAA AATTTGCTAA AGCATGACAG TAATTATGCT 
AAAATGTATT GCAATCCAGA TGCTATGTCT TCTCTCACAG AGAAAACTAT TTATTCCGGA 
GAGGAGAACA TGGACATTAC CAAGAGTCAT ACAGTTGCAA TAGATAATCA AATTTTTAAA 
CAAGATCAAT CAAATGTGCA AATAGCAGCT GCACCAACAC CCGAAAAAGA AATGATGCTC 
CAAAATCTTA TGACCACATC AGAAGATGGG AAAATGAATG TAAATTGTAA CTCAGTTCCT 
CATGTATCTA AGGAAAGAAT ACAGCAGAGC CTGTCAAATC CTTTGTCTAT TTCATTGACT 
GATAGAAAGA CTGAACTCTT ATCAGGTGAA AATATGGATT TGACTGAAAG TCACACAAGT 
AACTTAGGAA GTCAGGTTCC TCTTGCAGCT TATAATCTAG CACCGGAGAG TACCAGTGAA 
TCTCACTCTC AGAGCAAAAG CTCTTCAGAT GAATGTGAAG AAATTACCAA AAGTCGTAAT 
GAACCATTTC AGCGATCAGA CATAATAGCC AAAAACAGCT TAACCGACAC CTGGAACAAA 
GACAAAGATT GGGTTTTGAA GATTTTGCCC TACCTTGATA AAGATTCTCC TCAGTCAGCT 
GATTGTAATC AGGAGATAGC AACAAGCCAT AATATAGTCT ACTGTGGTGG AGTTCTTGAT 
AAACAAATAA CTAATAGAAA TACAGTATCA TGGGAACAAT CTTTGTTTTC TACCACAAAG 
CCATTATTTT CATCAGGACA GTTCTCTATG AAAAATCATG ATACTGCTAT AAGTAGTCAT 
ACAGTGAAAT CTGTACTAGG CCAGAATTCT AAACTGGCTG AGCCACTGAG GAAAAGTTTA 
AGCAATCCCA CACCTGACTA TTGCCATGAC AAGATGATTA TATGTTCAGA GGAAGAGCAA 
AATATGGATC TAACAAAGAG CCACACTGTC GTCATTGGAT TTGGTCCTTC TGAACTACAA 
GAACTTGGTA AAACTAATTT AGAACACACT ACTGGCCAGC TAACAACAAT GAACAGACAG 
ATAGCTGTAA AAGTTGAAAA ATGTGGTAAA AGTCCCATAG AAAAAAGTGG AGTGCTTAAA 
TCTAACTGTA TTATGGATGT GTTAGAGGAC GAAAGTGTAC AGAAACCTAA ATTTCCAAAG 
GAAAAGCAAA ATGTCAAAAT TTGGGGAAGG AAAAGTGTTG GTGGACCAAA AATTGATAAG 
ACTATTGTAT TTTCAGAAGA CGATAAGAAT GATATGGATA TCACTAAGAG TTATACAATA 
GAAATAAACC ATAGACCTTT ATTAGAGAAA CGTGATTGTC ATTTGGTGCC ATTGGCAGGA 
ACTTCTGAAA CTATTTTATA TACATGTAGG CAGGATGACA TGGAGATCAC TAGAAGTCAC 
ACAACTGCCT TAGAATGTAA AACTGTCTCA CCAGATGAAA TAACTACTAG GCCTATGGAC 
AAAACTGTAG TGTTTGTAGA TAATCATGTT GAACTAGAAA TGACAGAGTC CCATACTGTT 
TTCATTGACT ACCAAGAAAA GGAAAGAACA GACAGACCTA ACTTTGAACT ATCCCAAAGG 
AAAAGCCTAG GAACACCAAC AGTGATATGT ACTCCTACTG AGGAGAGTGT TTTCTTTCCA 
GGAAATGGTG AAAGTGACCG TCTAGTAGCA AATGACAGCC AGCTAACCCC TCTGGAGGAA 
TGGTCTAATA ATAGGGGCCC TGTAGAGGTA GCTGATAACA TGGAATTGTC TAAATCAGCC 
ACTTGCAAAA ACATCAAAGA TGTACAAAGT CCTGGATTTC TGAATGAACC TCTATCAAGC 
AAAAGTCAGA GAAGAAAAAG CCTTAAGCTA AAAAATGACA AGACCATTGT ATTTTCAGAG 
AATCATAAAA ATGATATGGA TATTACCCAG AGTTGTATGG TGGAAATAGA TAACGAAAGT 
GCCCTGGAGG ATAAAGAGGA CTTCCATTTG GCAGGGGCTT CTAAAACTAT TTTGTATTCA 
TGTGGGCAGG ATGACATGGA GATCACTAGG AGTCACACAA CTGCCTTAGA ATGTAAAACT 
CTCCTGCCAA ATGAAATAGC TATTAGGCCC ATGGACAAAA CCGTATTGTT CACAGATAAT 
TACAGTGATC TGGAAGTCAC CGATTCCCAT ACTGTTTTCA TTGACTGTCA AGCCACAGAG 
AAAATACTTG AAGAAAACCC TAAATTTGGA ATAGGAAAAG GAAAAAACTT GGGTGTTTCC 
TTTCCTAAGG ATAATAGCTG TGTTCAAGAA ATCGCTGAAA AACAAGCACT GGCTGTAGGA 
AACAAAATAG TTCTTCACAC CGAGCAAAAG CAACAACTCT TTGCTGCTAC TAATAGAACT 
ACTAATGAAA TCATCAAATT TCATAGTGCT GCTATGGATG AAAAGGTCAT AGGGAAAGTT 
GTAGACCAGG CCTGTACATT GGAAAAAGCG CAAGTTGAAA GCTGTCAGTT AAATAATAGA 
GATAGAAGAA ATGTGGACTT TACAAGTAGT CATGCAACTG CTGTTTGTGG ATCCAGTGAT 
AATTATTCCT GTTTACCAAA TGTTATTTCC TGTACTGATA ATTTGGAGGG TAGTGCCATG 
CTCTTATGTG ATAAAGATGA GGAAAAAGCC AATTATTGCC CAGTGCAAAA TGATCTTGCT 
TATGCAAATG ATTTTGCCAG TGAATATTAC TTGGAATCTG AGGGACAGCC TCTCTCTGCT 
CCTTGTCCTT TGTTAGAGAA GGAAGAAGTT ATTCAAACCA GTACCAAAGG ACAGTTAGAC 
TGTGTTATAA CACTGCACAA AGATCAAGAT CTGATTAAGG ATCCACGAAA TCTATTGGCT 
AATCAAACTT TAGTATATAG TCAAGATCTG GGGGAGATGA CTAAACTTAA TTCAAAGCGA 
GTATCTTTTA AGCTTCCAAA GGATCAAATG AAAGTCTATG TTGATGACAT TTATGTTATT 
CCTCAGCCTC ATTTCTCAAC CGACCAACCT CCATTACCTA AAAAAGGACA GAGTAGTATC 
AATAAAGAAG AAGTAATACT GTCTAAAGCT GGAAATAAGA GTTTAAATAT TATAGAAAAT 
TCCTCTGCAC CCATATGTGA AAACAAGCCC AAAATACTCA ATAGTGAGGA ATGGTTTGCT 
GCAGCCTGTA AAAAAGAACT GAAGGAAAAT ATTCAAACAA CTAACTATAA TACAGCTCTA 
GATTTCCACA GTAACTCAGA CGTAACTAAG CAAGTCATTC AAACTCATGT CAATGCTGGA 
GAAGCACCAG ATCCTGTAAT TACATCTAAT GTTCCATGTT TTCATAGTAT CAAACCAAAT 
CTGAATAATT TGAATGGAAA AACTGGAGAG TTTTTAGCCT TTCAAACTGT TCATCTACCA 
CCCCTTCCAG AGCAATTACT TGAATTAGGA AATAAGGCAC ACAATGATAT GCATATAGTG 
CAAGCTACAG AAATACATAA TATTAACATA ATCTCCAGCA ATGCTAAAGA TAGTAGAGAT 
GAGGAAAATA AAAAGTCTCA TAATGGAGCT GAAACCACCT CTCTACCGCC AAAGACAGTT 
TTTAAAGATA AAGTAAGGAG ATGTTCTTTG GGAATCTTTT TGCCTAGATT GCCCAACAAG 
AGAAATTGTA GTGTCACTGG TATTGATGAC CTGGAACAGA TTCCAGCAGA CACAACTGAT 
ATAAATCACT TAGAAACTCA GCCGGTCTCT AGCAAAGATT CAGGCATTGG ATCTGTTGCA 
GGTAAACTGA ACCTAAGTCC TTCTCAATAT ATAAATGAGG AAAATCTTCC TGTATATCCT 
GATGAGATCA ATTCTTCAGA CTCTATTAAC ATAGAAACTG AGGAAAAGGC CTTGATTGAG 
ACATACCAAA AAGAGATTTC ACCATATGAA AATAAAATGG GAAAAACTTG CAATAGCCAA 
AAAAGAACGT GGGTACAAGA AGAAGAAGAT ATTCATAAGG AGAAAAAAAT CAGAAAAAAT 
GAGATTAAGT TTAGTGATAC GACACAAGAT CGGGAGATTT TTGATCACCA TACTGAAGAG 
GATATAGATA AAAGTGCTAA CAGTGTATTG ATAAAAAACC TGAGCAGGAC CCCATCTAGT 
TGCAGCAGCT CTCTGGATTC AATCAAGGCT GATGGGACCT CTCTGGACTT CAGCACTTAC 
CGCAGTAGTC AAATGGAATC ACAGTTTCTC AGAGATACTA TTTGTGAAGA GAGCTTGAGG 
GAGAAACTCC AAGATGGGAG AATAACAATA AGGGAGTTCT TTATACTTCT CCAGGTCCAC 
ATCTTGATAC AGAAACCCCG ACAGAGCAAT CTCCCAGGCA ATTTTACTGT AAACACACCA 
CCTACTCCAG AAGACCTGAT GTTAAGTCAA TATGTTTACC GACCCAAGAT ACAGATTTAT 
AGAGAAGATT GTGAGGCTCG TCGCCAAAAG ATTGAAGAAT TAAAGCTTTC TGCATCGAAC 
CAAGATAAGC TGTTGGTTGA TATAAATAAG AACCTGTGGG AAAAAATGAG ACACTGCTCT 
GACAAAGAGC TGAAGGCCTT TGGAATTTAT CTTAACAAAA TAAAGTCATG TTTTACCAAG 
ATGACTAAAG TCTTCACTCA CCAAGGAAAA GTGGCTCTGT ATGGCAAGCT GGTGCAGTCA 
GCTCAGAATG AGAGGGAGAA ACTTCAAATA AAGATAGATG AGATGGATAA AATACTTAAG 
AAGATCGATA ACTGCCTCAC TGAGATGGAA ACAGAAACTA AGAATTTGGA GGATGAAGAG 
AAAAACAATC CTGTGGAAGA ATGGGATTCT GAAATGAGAG CTGCAGAAAA AGAATTGGAA 
CAGCTGAAAA CTGAAGAAGA GGAGCTTCAA AGAAATCTCT TAGAACTGGA GGTACAAAAA 
GAGCAGACCC TTGCTCAAAT AGACTTTATG CAAAAACAAA GAAATAGAAC TGAAGAGCTA 
CTGGATCAGT TGAGCTTGTC TGAGTGGGAT GTCGTTGAGT GGAGTGATGA TCAAGCTGTA 
TTCACCTTTG TTTATGACAC GATACAACTC ACCATCACCT TTGAAGAGTC AGTTGTTGGT 
TTCCCTTTCC TGGACAAGCG TTATAGGAAG ATTGTTGATG TCAATTTTCA ATCTCTGTTA 
GATGAGGATC AAGCTCCTCC TTCCTCCCTT TTAGTTCATA AGCTTATTTT CCAGTACGTT 
GAAGAAAAGG AATCCTGGAA GAAGACATGT ACAACCCAGC ATCAGTTACC CAAGATGCTT 
GAAGAATTCT CACTGGTAGT GCACCATTGC AGACTCCTTG GAGAGGAGAT TGAGTATTTA 
AAGAGATGGG GACCAAATTA TAACCTAATG AACATAGATA TTAATAATAA TGAATTGAGA 
CTTTTATTCT CTAGCTCCGC AGCATTTGCA AAGTTTGAAA TAACTTTGTT TCTCTCAGCC 
TATTATCCAT CTGTACCATT ACCTTCCACC ATTCAGAATC ACGTTGGGAA CACTAGCCAA 
GATGATATTG CTACCATTCT ATCTAAAGTG CCACTGGAGA ACAACTACCT GAAGAATGTA 
GTCAAGCAAA TTTACCAAGA TCTGTTTCAG GACTGCCATT TCTACCACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_653091.3
CDS	162..7112
Translation	MDGVSSEANEENDNIERPVRRRHSSILKPPRSPLQDLRGGNERVQESNALRNKKNSRRVSFADTIKVFQTESHMKIVRKSEMEETETGENLLLIQNKKLEDNYCEITGMNTLLSAPIHTQMQQKEFSIIEHTRERKHANDQTVIFSDENQMDLTSSHTVMITKGLLDNPISEKSTKIDTTSFLANLKLHTEDSRMKKEVNFSVDQNTSSENKIDFNDFIKRLKTGKCSAFPDVPDKENFEIPIYSKEPNSASSTHQMHVSLKEDENNSNITRLFREKDDGMNFTQCHTANIQTLIPTSSETNSRESKGNDITIYGNDFMDLTFNHTLQILPATGNFSEIENQTQNAMDVTTGYGTKASGNKTVFKSKQNTAFQDLSINSADKIHITRSHIMGAETHIVSQTCNQDARILAMTPESIYSNPSIQGCKTVFYSSCNDAMEMTKCLSNMREEKNLLKHDSNYAKMYCNPDAMSSLTEKTIYSGEENMDITKSHTVAIDNQIFKQDQSNVQIAAAPTPEKEMMLQNLMTTSEDGKMNVNCNSVPHVSKERIQQSLSNPLSISLTDRKTELLSGENMDLTESHTSNLGSQVPLAAYNLAPESTSESHSQSKSSSDECEEITKSRNEPFQRSDIIAKNSLTDTWNKDKDWVLKILPYLDKDSPQSADCNQEIATSHNIVYCGGVLDKQITNRNTVSWEQSLFSTTKPLFSSGQFSMKNHDTAISSHTVKSVLGQNSKLAEPLRKSLSNPTPDYCHDKMIICSEEEQNMDLTKSHTVVIGFGPSELQELGKTNLEHTTGQLTTMNRQIAVKVEKCGKSPIEKSGVLKSNCIMDVLEDESVQKPKFPKEKQNVKIWGRKSVGGPKIDKTIVFSEDDKNDMDITKSYTIEINHRPLLEKRDCHLVPLAGTSETILYTCRQDDMEITRSHTTALECKTVSPDEITTRPMDKTVVFVDNHVELEMTESHTVFIDYQEKERTDRPNFELSQRKSLGTPTVICTPTEESVFFPGNGESDRLVANDSQLTPLEEWSNNRGPVEVADNMELSKSATCKNIKDVQSPGFLNEPLSSKSQRRKSLKLKNDKTIVFSENHKNDMDITQSCMVEIDNESALEDKEDFHLAGASKTILYSCGQDDMEITRSHTTALECKTLLPNEIAIRPMDKTVLFTDNYSDLEVTDSHTVFIDCQATEKILEENPKFGIGKGKNLGVSFPKDNSCVQEIAEKQALAVGNKIVLHTEQKQQLFAATNRTTNEIIKFHSAAMDEKVIGKVVDQACTLEKAQVESCQLNNRDRRNVDFTSSHATAVCGSSDNYSCLPNVISCTDNLEGSAMLLCDKDEEKANYCPVQNDLAYANDFASEYYLESEGQPLSAPCPLLEKEEVIQTSTKGQLDCVITLHKDQDLIKDPRNLLANQTLVYSQDLGEMTKLNSKRVSFKLPKDQMKVYVDDIYVIPQPHFSTDQPPLPKKGQSSINKEEVILSKAGNKSLNIIENSSAPICENKPKILNSEEWFAAACKKELKENIQTTNYNTALDFHSNSDVTKQVIQTHVNAGEAPDPVITSNVPCFHSIKPNLNNLNGKTGEFLAFQTVHLPPLPEQLLELGNKAHNDMHIVQATEIHNINIISSNAKDSRDEENKKSHNGAETTSLPPKTVFKDKVRRCSLGIFLPRLPNKRNCSVTGIDDLEQIPADTTDINHLETQPVSSKDSGIGSVAGKLNLSPSQYINEENLPVYPDEINSSDSINIETEEKALIETYQKEISPYENKMGKTCNSQKRTWVQEEEDIHKEKKIRKNEIKFSDTTQDREIFDHHTEEDIDKSANSVLIKNLSRTPSSCSSSLDSIKADGTSLDFSTYRSSQMESQFLRDTICEESLREKLQDGRITIREFFILLQVHILIQKPRQSNLPGNFTVNTPPTPEDLMLSQYVYRPKIQIYREDCEARRQKIEELKLSASNQDKLLVDINKNLWEKMRHCSDKELKAFGIYLNKIKSCFTKMTKVFTHQGKVALYGKLVQSAQNEREKLQIKIDEMDKILKKIDNCLTEMETETKNLEDEEKNNPVEEWDSEMRAAEKELEQLKTEEEELQRNLLELEVQKEQTLAQIDFMQKQRNRTEELLDQLSLSEWDVVEWSDDQAVFTFVYDTIQLTITFEESVVGFPFLDKRYRKIVDVNFQSLLDEDQAPPSSLLVHKLIFQYVEEKESWKKTCTTQHQLPKMLEEFSLVVHHCRLLGEEIEYLKRWGPNYNLMNIDINNNELRLLFSSSAAFAKFEITLFLSAYYPSVPLPSTIQNHVGNTSQDDIATILSKVPLENNYLKNVVKQIYQDLFQDCHFYH

Target ORF information:

RefSeq Version	NM_144508.5
Organism	Homo sapiens(human)
Definition	Homo sapiens kinetochore scaffold 1 (KNL1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_144508.5

ORF Insert Sequence:

ATGGATGGGG TGTCTTCAGA GGCTAATGAA GAAAATGACA ATATAGAGAG ACCTGTTAGA 
AGACGGCATT CTTCAATATT GAAACCCCCA AGGAGTCCTC TTCAGGACCT CAGAGGTGGG 
AATGAAAGAG TTCAGGAATC CAATGCTTTG AGAAATAAGA AAAACTCTCG TCGAGTCAGC 
TTTGCAGATA CTATAAAGGT ATTCCAGACG GAGTCTCATA TGAAAATAGT GAGAAAGTCA 
GAAATGGAAG AAACAGAAAC AGGAGAAAAT CTTCTTTTGA TACAGAATAA GAAATTAGAA 
GATAATTACT GTGAAATTAC TGGGATGAAC ACATTGCTTT CTGCTCCCAT TCATACCCAG 
ATGCAACAGA AGGAGTTTTC AATTATAGAA CATACCCGTG AAAGGAAACA TGCAAATGAC 
CAGACAGTCA TTTTTTCAGA TGAAAACCAG ATGGACCTGA CATCAAGTCA CACTGTAATG 
ATTACCAAAG GCCTTTTAGA TAATCCCATA AGTGAAAAGT CCACCAAGAT AGATACCACA 
TCATTTCTAG CTAATTTAAA GCTTCACACC GAGGACTCAA GAATGAAAAA AGAAGTAAAT 
TTTTCCGTGG ATCAAAACAC TTCTTCAGAA AATAAAATAG ATTTCAATGA CTTCATAAAA 
AGATTGAAAA CAGGAAAATG TAGTGCTTTT CCTGATGTGC CTGATAAAGA AAATTTTGAG 
ATACCTATTT ATTCCAAGGA ACCGAACAGT GCCTCTTCTA CACATCAAAT GCATGTATCT 
CTTAAGGAAG ATGAAAATAA CAGTAATATT ACTAGGCTCT TTAGAGAAAA AGATGATGGG 
ATGAATTTCA CCCAGTGTCA TACAGCCAAT ATTCAGACAT TGATTCCCAC ATCCAGTGAG 
ACCAACTCAC GGGAATCTAA AGGTAATGAT ATTACAATTT ATGGCAATGA CTTTATGGAC 
TTGACATTTA ACCACACTTT GCAGATCTTA CCTGCAACAG GTAATTTTTC TGAAATAGAA 
AATCAAACTC AGAATGCCAT GGATGTAACA ACAGGTTATG GAACTAAAGC TTCAGGAAAT 
AAAACAGTTT TTAAGAGTAA ACAAAATACT GCTTTTCAAG ACCTTTCCAT AAACTCTGCA 
GACAAAATAC ATATTACCAG AAGTCATATT ATGGGGGCAG AAACTCACAT AGTCTCACAG 
ACTTGTAATC AGGATGCCAG AATATTAGCC ATGACCCCAG AATCTATATA TTCTAATCCA 
TCTATTCAAG GTTGTAAGAC TGTTTTCTAT TCTAGTTGTA ATGATGCCAT GGAAATGACC 
AAATGTCTCT CAAATATGAG AGAGGAGAAA AATTTGCTAA AGCATGACAG TAATTATGCT 
AAAATGTATT GCAATCCAGA TGCTATGTCT TCTCTCACAG AGAAAACTAT TTATTCCGGA 
GAGGAGAACA TGGACATTAC CAAGAGTCAT ACAGTTGCAA TAGATAATCA AATTTTTAAA 
CAAGATCAAT CAAATGTGCA AATAGCAGCT GCACCAACAC CCGAAAAAGA AATGATGCTC 
CAAAATCTTA TGACCACATC AGAAGATGGG AAAATGAATG TAAATTGTAA CTCAGTTCCT 
CATGTATCTA AGGAAAGAAT ACAGCAGAGC CTGTCAAATC CTTTGTCTAT TTCATTGACT 
GATAGAAAGA CTGAACTCTT ATCAGGTGAA AATATGGATT TGACTGAAAG TCACACAAGT 
AACTTAGGAA GTCAGGTTCC TCTTGCAGCT TATAATCTAG CACCGGAGAG TACCAGTGAA 
TCTCACTCTC AGAGCAAAAG CTCTTCAGAT GAATGTGAAG AAATTACCAA AAGTCGTAAT 
GAACCATTTC AGCGATCAGA CATAATAGCC AAAAACAGCT TAACCGACAC CTGGAACAAA 
GACAAAGATT GGGTTTTGAA GATTTTGCCC TACCTTGATA AAGATTCTCC TCAGTCAGCT 
GATTGTAATC AGGAGATAGC AACAAGCCAT AATATAGTCT ACTGTGGTGG AGTTCTTGAT 
AAACAAATAA CTAATAGAAA TACAGTATCA TGGGAACAAT CTTTGTTTTC TACCACAAAG 
CCATTATTTT CATCAGGACA GTTCTCTATG AAAAATCATG ATACTGCTAT AAGTAGTCAT 
ACAGTGAAAT CTGTACTAGG CCAGAATTCT AAACTGGCTG AGCCACTGAG GAAAAGTTTA 
AGCAATCCCA CACCTGACTA TTGCCATGAC AAGATGATTA TATGTTCAGA GGAAGAGCAA 
AATATGGATC TAACAAAGAG CCACACTGTC GTCATTGGAT TTGGTCCTTC TGAACTACAA 
GAACTTGGTA AAACTAATTT AGAACACACT ACTGGCCAGC TAACAACAAT GAACAGACAG 
ATAGCTGTAA AAGTTGAAAA ATGTGGTAAA AGTCCCATAG AAAAAAGTGG AGTGCTTAAA 
TCTAACTGTA TTATGGATGT GTTAGAGGAC GAAAGTGTAC AGAAACCTAA ATTTCCAAAG 
GAAAAGCAAA ATGTCAAAAT TTGGGGAAGG AAAAGTGTTG GTGGACCAAA AATTGATAAG 
ACTATTGTAT TTTCAGAAGA CGATAAGAAT GATATGGATA TCACTAAGAG TTATACAATA 
GAAATAAACC ATAGACCTTT ATTAGAGAAA CGTGATTGTC ATTTGGTGCC ATTGGCAGGA 
ACTTCTGAAA CTATTTTATA TACATGTAGG CAGGATGACA TGGAGATCAC TAGAAGTCAC 
ACAACTGCCT TAGAATGTAA AACTGTCTCA CCAGATGAAA TAACTACTAG GCCTATGGAC 
AAAACTGTAG TGTTTGTAGA TAATCATGTT GAACTAGAAA TGACAGAGTC CCATACTGTT 
TTCATTGACT ACCAAGAAAA GGAAAGAACA GACAGACCTA ACTTTGAACT ATCCCAAAGG 
AAAAGCCTAG GAACACCAAC AGTGATATGT ACTCCTACTG AGGAGAGTGT TTTCTTTCCA 
GGAAATGGTG AAAGTGACCG TCTAGTAGCA AATGACAGCC AGCTAACCCC TCTGGAGGAA 
TGGTCTAATA ATAGGGGCCC TGTAGAGGTA GCTGATAACA TGGAATTGTC TAAATCAGCC 
ACTTGCAAAA ACATCAAAGA TGTACAAAGT CCTGGATTTC TGAATGAACC TCTATCAAGC 
AAAAGTCAGA GAAGAAAAAG CCTTAAGCTA AAAAATGACA AGACCATTGT ATTTTCAGAG 
AATCATAAAA ATGATATGGA TATTACCCAG AGTTGTATGG TGGAAATAGA TAACGAAAGT 
GCCCTGGAGG ATAAAGAGGA CTTCCATTTG GCAGGGGCTT CTAAAACTAT TTTGTATTCA 
TGTGGGCAGG ATGACATGGA GATCACTAGG AGTCACACAA CTGCCTTAGA ATGTAAAACT 
CTCCTGCCAA ATGAAATAGC TATTAGGCCC ATGGACAAAA CCGTATTGTT CACAGATAAT 
TACAGTGATC TGGAAGTCAC CGATTCCCAT ACTGTTTTCA TTGACTGTCA AGCCACAGAG 
AAAATACTTG AAGAAAACCC TAAATTTGGA ATAGGAAAAG GAAAAAACTT GGGTGTTTCC 
TTTCCTAAGG ATAATAGCTG TGTTCAAGAA ATCGCTGAAA AACAAGCACT GGCTGTAGGA 
AACAAAATAG TTCTTCACAC CGAGCAAAAG CAACAACTCT TTGCTGCTAC TAATAGAACT 
ACTAATGAAA TCATCAAATT TCATAGTGCT GCTATGGATG AAAAGGTCAT AGGGAAAGTT 
GTAGACCAGG CCTGTACATT GGAAAAAGCG CAAGTTGAAA GCTGTCAGTT AAATAATAGA 
GATAGAAGAA ATGTGGACTT TACAAGTAGT CATGCAACTG CTGTTTGTGG ATCCAGTGAT 
AATTATTCCT GTTTACCAAA TGTTATTTCC TGTACTGATA ATTTGGAGGG TAGTGCCATG 
CTCTTATGTG ATAAAGATGA GGAAAAAGCC AATTATTGCC CAGTGCAAAA TGATCTTGCT 
TATGCAAATG ATTTTGCCAG TGAATATTAC TTGGAATCTG AGGGACAGCC TCTCTCTGCT 
CCTTGTCCTT TGTTAGAGAA GGAAGAAGTT ATTCAAACCA GTACCAAAGG ACAGTTAGAC 
TGTGTTATAA CACTGCACAA AGATCAAGAT CTGATTAAGG ATCCACGAAA TCTATTGGCT 
AATCAAACTT TAGTATATAG TCAAGATCTG GGGGAGATGA CTAAACTTAA TTCAAAGCGA 
GTATCTTTTA AGCTTCCAAA GGATCAAATG AAAGTCTATG TTGATGACAT TTATGTTATT 
CCTCAGCCTC ATTTCTCAAC CGACCAACCT CCATTACCTA AAAAAGGACA GAGTAGTATC 
AATAAAGAAG AAGTAATACT GTCTAAAGCT GGAAATAAGA GTTTAAATAT TATAGAAAAT 
TCCTCTGCAC CCATATGTGA AAACAAGCCC AAAATACTCA ATAGTGAGGA ATGGTTTGCT 
GCAGCCTGTA AAAAAGAACT GAAGGAAAAT ATTCAAACAA CTAACTATAA TACAGCTCTA 
GATTTCCACA GTAACTCAGA CGTAACTAAG CAAGTCATTC AAACTCATGT CAATGCTGGA 
GAAGCACCAG ATCCTGTAAT TACATCTAAT GTTCCATGTT TTCATAGTAT CAAACCAAAT 
CTGAATAATT TGAATGGAAA AACTGGAGAG TTTTTAGCCT TTCAAACTGT TCATCTACCA 
CCCCTTCCAG AGCAATTACT TGAATTAGGA AATAAGGCAC ACAATGATAT GCATATAGTG 
CAAGCTACAG AAATACATAA TATTAACATA ATCTCCAGCA ATGCTAAAGA TAGTAGAGAT 
GAGGAAAATA AAAAGTCTCA TAATGGAGCT GAAACCACCT CTCTACCGCC AAAGACAGTT 
TTTAAAGATA AAGTAAGGAG ATGTTCTTTG GGAATCTTTT TGCCTAGATT GCCCAACAAG 
AGAAATTGTA GTGTCACTGG TATTGATGAC CTGGAACAGA TTCCAGCAGA CACAACTGAT 
ATAAATCACT TAGAAACTCA GCCGGTCTCT AGCAAAGATT CAGGCATTGG ATCTGTTGCA 
GGTAAACTGA ACCTAAGTCC TTCTCAATAT ATAAATGAGG AAAATCTTCC TGTATATCCT 
GATGAGATCA ATTCTTCAGA CTCTATTAAC ATAGAAACTG AGGAAAAGGC CTTGATTGAG 
ACATACCAAA AAGAGATTTC ACCATATGAA AATAAAATGG GAAAAACTTG CAATAGCCAA 
AAAAGAACGT GGGTACAAGA AGAAGAAGAT ATTCATAAGG AGAAAAAAAT CAGAAAAAAT 
GAGATTAAGT TTAGTGATAC GACACAAGAT CGGGAGATTT TTGATCACCA TACTGAAGAG 
GATATAGATA AAAGTGCTAA CAGTGTATTG ATAAAAAACC TGAGCAGGAC CCCATCTAGT 
TGCAGCAGCT CTCTGGATTC AATCAAGGCT GATGGGACCT CTCTGGACTT CAGCACTTAC 
CGCAGTAGTC AAATGGAATC ACAGTTTCTC AGAGATACTA TTTGTGAAGA GAGCTTGAGG 
GAGAAACTCC AAGATGGGAG AATAACAATA AGGGAGTTCT TTATACTTCT CCAGGTCCAC 
ATCTTGATAC AGAAACCCCG ACAGAGCAAT CTCCCAGGCA ATTTTACTGT AAACACACCA 
CCTACTCCAG AAGACCTGAT GTTAAGTCAA TATGTTTACC GACCCAAGAT ACAGATTTAT 
AGAGAAGATT GTGAGGCTCG TCGCCAAAAG ATTGAAGAAT TAAAGCTTTC TGCATCGAAC 
CAAGATAAGC TGTTGGTTGA TATAAATAAG AACCTGTGGG AAAAAATGAG ACACTGCTCT 
GACAAAGAGC TGAAGGCCTT TGGAATTTAT CTTAACAAAA TAAAGTCATG TTTTACCAAG 
ATGACTAAAG TCTTCACTCA CCAAGGAAAA GTGGCTCTGT ATGGCAAGCT GGTGCAGTCA 
GCTCAGAATG AGAGGGAGAA ACTTCAAATA AAGATAGATG AGATGGATAA AATACTTAAG 
AAGATCGATA ACTGCCTCAC TGAGATGGAA ACAGAAACTA AGAATTTGGA GGATGAAGAG 
AAAAACAATC CTGTGGAAGA ATGGGATTCT GAAATGAGAG CTGCAGAAAA AGAATTGGAA 
CAGCTGAAAA CTGAAGAAGA GGAGCTTCAA AGAAATCTCT TAGAACTGGA GGTACAAAAA 
GAGCAGACCC TTGCTCAAAT AGACTTTATG CAAAAACAAA GAAATAGAAC TGAAGAGCTA 
CTGGATCAGT TGAGCTTGTC TGAGTGGGAT GTCGTTGAGT GGAGTGATGA TCAAGCTGTA 
TTCACCTTTG TTTATGACAC GATACAACTC ACCATCACCT TTGAAGAGTC AGTTGTTGGT 
TTCCCTTTCC TGGACAAGCG TTATAGGAAG ATTGTTGATG TCAATTTTCA ATCTCTGTTA 
GATGAGGATC AAGCTCCTCC TTCCTCCCTT TTAGTTCATA AGCTTATTTT CCAGTACGTT 
GAAGAAAAGG AATCCTGGAA GAAGACATGT ACAACCCAGC ATCAGTTACC CAAGATGCTT 
GAAGAATTCT CACTGGTAGT GCACCATTGC AGACTCCTTG GAGAGGAGAT TGAGTATTTA 
AAGAGATGGG GACCAAATTA TAACCTAATG AACATAGATA TTAATAATAA TGAATTGAGA 
CTTTTATTCT CTAGCTCCGC AGCATTTGCA AAGTTTGAAA TAACTTTGTT TCTCTCAGCC 
TATTATCCAT CTGTACCATT ACCTTCCACC ATTCAGAATC ACGTTGGGAA CACTAGCCAA 
GATGATATTG CTACCATTCT ATCTAAAGTG CCACTGGAGA ACAACTACCT GAAGAATGTA 
GTCAAGCAAA TTTACCAAGA TCTGTTTCAG GACTGCCATT TCTACCACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KNL1 ( XM_017022432.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016877921.1 Homo sapiens kinetochore scaffold 1 (KNL1), transcript variant X1, mRNA.

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CloneID	OHu70263
Clone ID Related Accession (Same CDS sequence)	XM_017022432.1
Accession Version	XM_017022432.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	kinetochore scaffold 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACACAT TGCTTTCTGC TCCCATTCAT ACCCAGATGC AACAGAAGGA GTTTTCAATT 
ATAGAACATA CCCGTGAAAG GAAACATGCA AATGACCAGA CAGTCATTTT TTCAGATGAA 
AACCAGATGG ACCTGACATC AAGTCACACT GTAATGATTA CCAAAGGCCT TTTAGATAAT 
CCCATAAGTG AAAAGTCCAC CAAGATAGAT ACCACATCAT TTCTAGCTAA TTTAAAGCTT 
CACACCGAGG ACTCAAGAAT GAAAAAAGAA GTAAATTTTT CCGTGGATCA AAACACTTCT 
TCAGAAAATA AAATAGATTT CAATGACTTC ATAAAAAGAT TGAAAACAGG AAAATGTAGT 
GCTTTTCCTG ATGTGCCTGA TAAAGAAAAT TTTGAGATAC CTATTTATTC CAAGGAACCG 
AACAGTGCCT CTTCTACACA TCAAATGCAT GTATCTCTTA AGGAAGATGA AAATAACAGT 
AATATTACTA GGCTCTTTAG AGAAAAAGAT GATGGGATGA ATTTCACCCA GTGTCATACA 
GCCAATATTC AGACATTGAT TCCCACATCC AGTGAGACCA ACTCACGGGA ATCTAAAGGT 
AATGATATTA CAATTTATGG CAATGACTTT ATGGACTTGA CATTTAACCA CACTTTGCAG 
ATCTTACCTG CAACAGGTAA TTTTTCTGAA ATAGAAAATC AAACTCAGAA TGCCATGGAT 
GTAACAACAG GTTATGGAAC TAAAGCTTCA GGAAATAAAA CAGTTTTTAA GAGTAAACAA 
AATACTGCTT TTCAAGACCT TTCCATAAAC TCTGCAGACA AAATACATAT TACCAGAAGT 
CATATTATGG GGGCAGAAAC TCACATAGTC TCACAGACTT GTAATCAGGA TGCCAGAATA 
TTAGCCATGA CCCCAGAATC TATATATTCT AATCCATCTA TTCAAGGTTG TAAGACTGTT 
TTCTATTCTA GTTGTAATGA TGCCATGGAA ATGACCAAAT GTCTCTCAAA TATGAGAGAG 
GAGAAAAATT TGCTAAAGCA TGACAGTAAT TATGCTAAAA TGTATTGCAA TCCAGATGCT 
ATGTCTTCTC TCACAGAGAA AACTATTTAT TCCGGAGAGG AGAACATGGA CATTACCAAG 
AGTCATACAG TTGCAATAGA TAATCAAATT TTTAAACAAG ATCAATCAAA TGTGCAAATA 
GCAGCTGCAC CAACACCCGA AAAAGAAATG ATGCTCCAAA ATCTTATGAC CACATCAGAA 
GATGGGAAAA TGAATGTAAA TTGTAACTCA GTTCCTCATG TATCTAAGGA AAGAATACAG 
CAGAGCCTGT CAAATCCTTT GTCTATTTCA TTGACTGATA GAAAGACTGA ACTCTTATCA 
GGTGAAAATA TGGATTTGAC TGAAAGTCAC ACAAGTAACT TAGGAAGTCA GGTTCCTCTT 
GCAGCTTATA ATCTAGCACC GGAGAGTACC AGTGAATCTC ACTCTCAGAG CAAAAGCTCT 
TCAGATGAAT GTGAAGAAAT TACCAAAAGT CGTAATGAAC CATTTCAGCG ATCAGACATA 
ATAGCCAAAA ACAGCTTAAC CGACACCTGG AACAAAGACA AAGATTGGGT TTTGAAGATT 
TTGCCCTACC TTGATAAAGA TTCTCCTCAG TCAGCTGATT GTAATCAGGA GATAGCAACA 
AGCCATAATA TAGTCTACTG TGGTGGAGTT CTTGATAAAC AAATAACTAA TAGAAATACA 
GTATCATGGG AACAATCTTT GTTTTCTACC ACAAAGCCAT TATTTTCATC AGGACAGTTC 
TCTATGAAAA ATCATGATAC TGCTATAAGT AGTCATACAG TGAAATCTGT ACTAGGCCAG 
AATTCTAAAC TGGCTGAGCC ACTGAGGAAA AGTTTAAGCA ATCCCACACC TGACTATTGC 
CATGACAAGA TGATTATATG TTCAGAGGAA GAGCAAAATA TGGATCTAAC AAAGAGCCAC 
ACTGTCGTCA TTGGATTTGG TCCTTCTGAA CTACAAGAAC TTGGTAAAAC TAATTTAGAA 
CACACTACTG GCCAGCTAAC AACAATGAAC AGACAGATAG CTGTAAAAGT TGAAAAATGT 
GGTAAAAGTC CCATAGAAAA AAGTGGAGTG CTTAAATCTA ACTGTATTAT GGATGTGTTA 
GAGGACGAAA GTGTACAGAA ACCTAAATTT CCAAAGGAAA AGCAAAATGT CAAAATTTGG 
GGAAGGAAAA GTGTTGGTGG ACCAAAAATT GATAAGACTA TTGTATTTTC AGAAGACGAT 
AAGAATGATA TGGATATCAC TAAGAGTTAT ACAATAGAAA TAAACCATAG ACCTTTATTA 
GAGAAACGTG ATTGTCATTT GGTGCCATTG GCAGGAACTT CTGAAACTAT TTTATATACA 
TGTAGGCAGG ATGACATGGA GATCACTAGA AGTCACACAA CTGCCTTAGA ATGTAAAACT 
GTCTCACCAG ATGAAATAAC TACTAGGCCT ATGGACAAAA CTGTAGTGTT TGTAGATAAT 
CATGTTGAAC TAGAAATGAC AGAGTCCCAT ACTGTTTTCA TTGACTACCA AGAAAAGGAA 
AGAACAGACA GACCTAACTT TGAACTATCC CAAAGGAAAA GCCTAGGAAC ACCAACAGTG 
ATATGTACTC CTACTGAGGA GAGTGTTTTC TTTCCAGGAA ATGGTGAAAG TGACCGTCTA 
GTAGCAAATG ACAGCCAGCT AACCCCTCTG GAGGAATGGT CTAATAATAG GGGCCCTGTA 
GAGGTAGCTG ATAACATGGA ATTGTCTAAA TCAGCCACTT GCAAAAACAT CAAAGATGTA 
CAAAGTCCTG GATTTCTGAA TGAACCTCTA TCAAGCAAAA GTCAGAGAAG AAAAAGCCTT 
AAGCTAAAAA ATGACAAGAC CATTGTATTT TCAGAGAATC ATAAAAATGA TATGGATATT 
ACCCAGAGTT GTATGGTGGA AATAGATAAC GAAAGTGCCC TGGAGGATAA AGAGGACTTC 
CATTTGGCAG GGGCTTCTAA AACTATTTTG TATTCATGTG GGCAGGATGA CATGGAGATC 
ACTAGGAGTC ACACAACTGC CTTAGAATGT AAAACTCTCC TGCCAAATGA AATAGCTATT 
AGGCCCATGG ACAAAACCGT ATTGTTCACA GATAATTACA GTGATCTGGA AGTCACCGAT 
TCCCATACTG TTTTCATTGA CTGTCAAGCC ACAGAGAAAA TACTTGAAGA AAACCCTAAA 
TTTGGAATAG GAAAAGGAAA AAACTTGGGT GTTTCCTTTC CTAAGGATAA TAGCTGTGTT 
CAAGAAATCG CTGAAAAACA AGCACTGGCT GTAGGAAACA AAATAGTTCT TCACACCGAG 
CAAAAGCAAC AACTCTTTGC TGCTACTAAT AGAACTACTA ATGAAATCAT CAAATTTCAT 
AGTGCTGCTA TGGATGAAAA GGTCATAGGG AAAGTTGTAG ACCAGGCCTG TACATTGGAA 
AAAGCGCAAG TTGAAAGCTG TCAGTTAAAT AATAGAGATA GAAGAAATGT GGACTTTACA 
AGTAGTCATG CAACTGCTGT TTGTGGATCC AGTGATAATT ATTCCTGTTT ACCAAATGTT 
ATTTCCTGTA CTGATAATTT GGAGGGTAGT GCCATGCTCT TATGTGATAA AGATGAGGAA 
AAAGCCAATT ATTGCCCAGT GCAAAATGAT CTTGCTTATG CAAATGATTT TGCCAGTGAA 
TATTACTTGG AATCTGAGGG ACAGCCTCTC TCTGCTCCTT GTCCTTTGTT AGAGAAGGAA 
GAAGTTATTC AAACCAGTAC CAAAGGACAG TTAGACTGTG TTATAACACT GCACAAAGAT 
CAAGATCTGA TTAAGGATCC ACGAAATCTA TTGGCTAATC AAACTTTAGT ATATAGTCAA 
GATCTGGGGG AGATGACTAA ACTTAATTCA AAGCGAGTAT CTTTTAAGCT TCCAAAGGAT 
CAAATGAAAG TCTATGTTGA TGACATTTAT GTTATTCCTC AGCCTCATTT CTCAACCGAC 
CAACCTCCAT TACCTAAAAA AGGACAGAGT AGTATCAATA AAGAAGAAGT AATACTGTCT 
AAAGCTGGAA ATAAGAGTTT AAATATTATA GAAAATTCCT CTGCACCCAT ATGTGAAAAC 
AAGCCCAAAA TACTCAATAG TGAGGAATGG TTTGCTGCAG CCTGTAAAAA AGAACTGAAG 
GAAAATATTC AAACAACTAA CTATAATACA GCTCTAGATT TCCACAGTAA CTCAGACGTA 
ACTAAGCAAG TCATTCAAAC TCATGTCAAT GCTGGAGAAG CACCAGATCC TGTAATTACA 
TCTAATGTTC CATGTTTTCA TAGTATCAAA CCAAATCTGA ATAATTTGAA TGGAAAAACT 
GGAGAGTTTT TAGCCTTTCA AACTGTTCAT CTACCACCCC TTCCAGAGCA ATTACTTGAA 
TTAGGAAATA AGGCACACAA TGATATGCAT ATAGTGCAAG CTACAGAAAT ACATAATATT 
AACATAATCT CCAGCAATGC TAAAGATAGT AGAGATGAGG AAAATAAAAA GTCTCATAAT 
GGAGCTGAAA CCACCTCTCT ACCGCCAAAG ACAGTTTTTA AAGATAAAGT AAGGAGATGT 
TCTTTGGGAA TCTTTTTGCC TAGATTGCCC AACAAGAGAA ATTGTAGTGT CACTGGTATT 
GATGACCTGG AACAGATTCC AGCAGACACA ACTGATATAA ATCACTTAGA AACTCAGCCG 
GTCTCTAGCA AAGATTCAGG CATTGGATCT GTTGCAGGTA AACTGAACCT AAGTCCTTCT 
CAATATATAA ATGAGGAAAA TCTTCCTGTA TATCCTGATG AGATCAATTC TTCAGACTCT 
ATTAACATAG AAACTGAGGA AAAGGCCTTG ATTGAGACAT ACCAAAAAGA GATTTCACCA 
TATGAAAATA AAATGGGAAA AACTTGCAAT AGCCAAAAAA GAACGTGGGT ACAAGAAGAA 
GAAGATATTC ATAAGGAGAA AAAAATCAGA AAAAATGAGA TTAAGTTTAG TGATACGACA 
CAAGATCGGG AGATTTTTGA TCACCATACT GAAGAGGATA TAGATAAAAG TGCTAACAGT 
GTATTGATAA AAAACCTGAG CAGGACCCCA TCTAGTTGCA GCAGCTCTCT GGATTCAATC 
AAGGCTGATG GGACCTCTCT GGACTTCAGC ACTTACCGCA GTAGTCAAAT GGAATCACAG 
TTTCTCAGAG ATACTATTTG TGAAGAGAGC TTGAGGGAGA AACTCCAAGA TGGGAGAATA 
ACAATAAGGG AGTTCTTTAT ACTTCTCCAG GTCCACATCT TGATACAGAA ACCCCGACAG 
AGCAATCTCC CAGGCAATTT TACTGTAAAC ACACCACCTA CTCCAGAAGA CCTGATGTTA 
AGTCAATATG TTTACCGACC CAAGATACAG ATTTATAGAG AAGATTGTGA GGCTCGTCGC 
CAAAAGATTG AAGAATTAAA GCTTTCTGCA TCGAACCAAG ATAAGCTGTT GGTTGATATA 
AATAAGAACC TGTGGGAAAA AATGAGACAC TGCTCTGACA AAGAGCTGAA GGCCTTTGGA 
ATTTATCTTA ACAAAATAAA GTCATGTTTT ACCAAGATGA CTAAAGTCTT CACTCACCAA 
GGAAAAGTGG CTCTGTATGG CAAGCTGGTG CAGTCAGCTC AGAATGAGAG GGAGAAACTT 
CAAATAAAGA TAGATGAGAT GGATAAAATA CTTAAGAAGA TCGATAACTG CCTCACTGAG 
ATGGAAACAG AAACTAAGAA TTTGGAGGAT GAAGAGAAAA ACAATCCTGT GGAAGAATGG 
GATTCTGAAA TGAGAGCTGC AGAAAAAGAA TTGGAACAGC TGAAAACTGA AGAAGAGGAG 
CTTCAAAGAA ATCTCTTAGA ACTGGAGGTA CAAAAAGAGC AGACCCTTGC TCAAATAGAC 
TTTATGCAAA AACAAAGAAA TAGAACTGAA GAGCTACTGG ATCAGTTGAG CTTGTCTGAG 
TGGGATGTCG TTGAGTGGAG TGATGATCAA GCTGTATTCA CCTTTGTTTA TGACACGATA 
CAACTCACCA TCACCTTTGA AGAGTCAGTT GTTGGTTTCC CTTTCCTGGA CAAGCGTTAT 
AGGAAGATTG TTGATGTCAA TTTTCAATCT CTGTTAGATG AGGATCAAGC TCCTCCTTCC 
TCCCTTTTAG TTCATAAGCT TATTTTCCAG TACGTTGAAG AAAAGGAATC CTGGAAGAAG 
ACATGTACAA CCCAGCATCA GTTACCCAAG ATGCTTGAAG AATTCTCACT GGTAGTGCAC 
CATTGCAGAC TCCTTGGAGA GGAGATTGAG TATTTAAAGA GATGGGGACC AAATTATAAC 
CTAATGAACA TAGATATTAA TAATAATGAA TTGAGACTTT TATTCTCTAG CTCCGCAGCA 
TTTGCAAAGT TTGAAATAAC TTTGTTTCTC TCAGCCTATT ATCCATCTGT ACCATTACCT 
TCCACCATTC AGAATCACGT TGGGAACACT AGCCAAGATG ATATTGCTAC CATTCTATCT 
AAAGTGCCAC TGGAGAACAA CTACCTGAAG AATGTAGTCA AGCAAATTTA CCAAGATCTG 
TTTCAGGACT GCCATTTCTA CCACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016877921.1
CDS	689..7315
Translation	MNTLLSAPIHTQMQQKEFSIIEHTRERKHANDQTVIFSDENQMDLTSSHTVMITKGLLDNPISEKSTKIDTTSFLANLKLHTEDSRMKKEVNFSVDQNTSSENKIDFNDFIKRLKTGKCSAFPDVPDKENFEIPIYSKEPNSASSTHQMHVSLKEDENNSNITRLFREKDDGMNFTQCHTANIQTLIPTSSETNSRESKGNDITIYGNDFMDLTFNHTLQILPATGNFSEIENQTQNAMDVTTGYGTKASGNKTVFKSKQNTAFQDLSINSADKIHITRSHIMGAETHIVSQTCNQDARILAMTPESIYSNPSIQGCKTVFYSSCNDAMEMTKCLSNMREEKNLLKHDSNYAKMYCNPDAMSSLTEKTIYSGEENMDITKSHTVAIDNQIFKQDQSNVQIAAAPTPEKEMMLQNLMTTSEDGKMNVNCNSVPHVSKERIQQSLSNPLSISLTDRKTELLSGENMDLTESHTSNLGSQVPLAAYNLAPESTSESHSQSKSSSDECEEITKSRNEPFQRSDIIAKNSLTDTWNKDKDWVLKILPYLDKDSPQSADCNQEIATSHNIVYCGGVLDKQITNRNTVSWEQSLFSTTKPLFSSGQFSMKNHDTAISSHTVKSVLGQNSKLAEPLRKSLSNPTPDYCHDKMIICSEEEQNMDLTKSHTVVIGFGPSELQELGKTNLEHTTGQLTTMNRQIAVKVEKCGKSPIEKSGVLKSNCIMDVLEDESVQKPKFPKEKQNVKIWGRKSVGGPKIDKTIVFSEDDKNDMDITKSYTIEINHRPLLEKRDCHLVPLAGTSETILYTCRQDDMEITRSHTTALECKTVSPDEITTRPMDKTVVFVDNHVELEMTESHTVFIDYQEKERTDRPNFELSQRKSLGTPTVICTPTEESVFFPGNGESDRLVANDSQLTPLEEWSNNRGPVEVADNMELSKSATCKNIKDVQSPGFLNEPLSSKSQRRKSLKLKNDKTIVFSENHKNDMDITQSCMVEIDNESALEDKEDFHLAGASKTILYSCGQDDMEITRSHTTALECKTLLPNEIAIRPMDKTVLFTDNYSDLEVTDSHTVFIDCQATEKILEENPKFGIGKGKNLGVSFPKDNSCVQEIAEKQALAVGNKIVLHTEQKQQLFAATNRTTNEIIKFHSAAMDEKVIGKVVDQACTLEKAQVESCQLNNRDRRNVDFTSSHATAVCGSSDNYSCLPNVISCTDNLEGSAMLLCDKDEEKANYCPVQNDLAYANDFASEYYLESEGQPLSAPCPLLEKEEVIQTSTKGQLDCVITLHKDQDLIKDPRNLLANQTLVYSQDLGEMTKLNSKRVSFKLPKDQMKVYVDDIYVIPQPHFSTDQPPLPKKGQSSINKEEVILSKAGNKSLNIIENSSAPICENKPKILNSEEWFAAACKKELKENIQTTNYNTALDFHSNSDVTKQVIQTHVNAGEAPDPVITSNVPCFHSIKPNLNNLNGKTGEFLAFQTVHLPPLPEQLLELGNKAHNDMHIVQATEIHNINIISSNAKDSRDEENKKSHNGAETTSLPPKTVFKDKVRRCSLGIFLPRLPNKRNCSVTGIDDLEQIPADTTDINHLETQPVSSKDSGIGSVAGKLNLSPSQYINEENLPVYPDEINSSDSINIETEEKALIETYQKEISPYENKMGKTCNSQKRTWVQEEEDIHKEKKIRKNEIKFSDTTQDREIFDHHTEEDIDKSANSVLIKNLSRTPSSCSSSLDSIKADGTSLDFSTYRSSQMESQFLRDTICEESLREKLQDGRITIREFFILLQVHILIQKPRQSNLPGNFTVNTPPTPEDLMLSQYVYRPKIQIYREDCEARRQKIEELKLSASNQDKLLVDINKNLWEKMRHCSDKELKAFGIYLNKIKSCFTKMTKVFTHQGKVALYGKLVQSAQNEREKLQIKIDEMDKILKKIDNCLTEMETETKNLEDEEKNNPVEEWDSEMRAAEKELEQLKTEEEELQRNLLELEVQKEQTLAQIDFMQKQRNRTEELLDQLSLSEWDVVEWSDDQAVFTFVYDTIQLTITFEESVVGFPFLDKRYRKIVDVNFQSLLDEDQAPPSSLLVHKLIFQYVEEKESWKKTCTTQHQLPKMLEEFSLVVHHCRLLGEEIEYLKRWGPNYNLMNIDINNNELRLLFSSSAAFAKFEITLFLSAYYPSVPLPSTIQNHVGNTSQDDIATILSKVPLENNYLKNVVKQIYQDLFQDCHFYH

Target ORF information:

RefSeq Version	XM_017022432.1
Organism	Homo sapiens(human)
Definition	Homo sapiens kinetochore scaffold 1 (KNL1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017022432.1

ORF Insert Sequence:

ATGAACACAT TGCTTTCTGC TCCCATTCAT ACCCAGATGC AACAGAAGGA GTTTTCAATT 
ATAGAACATA CCCGTGAAAG GAAACATGCA AATGACCAGA CAGTCATTTT TTCAGATGAA 
AACCAGATGG ACCTGACATC AAGTCACACT GTAATGATTA CCAAAGGCCT TTTAGATAAT 
CCCATAAGTG AAAAGTCCAC CAAGATAGAT ACCACATCAT TTCTAGCTAA TTTAAAGCTT 
CACACCGAGG ACTCAAGAAT GAAAAAAGAA GTAAATTTTT CCGTGGATCA AAACACTTCT 
TCAGAAAATA AAATAGATTT CAATGACTTC ATAAAAAGAT TGAAAACAGG AAAATGTAGT 
GCTTTTCCTG ATGTGCCTGA TAAAGAAAAT TTTGAGATAC CTATTTATTC CAAGGAACCG 
AACAGTGCCT CTTCTACACA TCAAATGCAT GTATCTCTTA AGGAAGATGA AAATAACAGT 
AATATTACTA GGCTCTTTAG AGAAAAAGAT GATGGGATGA ATTTCACCCA GTGTCATACA 
GCCAATATTC AGACATTGAT TCCCACATCC AGTGAGACCA ACTCACGGGA ATCTAAAGGT 
AATGATATTA CAATTTATGG CAATGACTTT ATGGACTTGA CATTTAACCA CACTTTGCAG 
ATCTTACCTG CAACAGGTAA TTTTTCTGAA ATAGAAAATC AAACTCAGAA TGCCATGGAT 
GTAACAACAG GTTATGGAAC TAAAGCTTCA GGAAATAAAA CAGTTTTTAA GAGTAAACAA 
AATACTGCTT TTCAAGACCT TTCCATAAAC TCTGCAGACA AAATACATAT TACCAGAAGT 
CATATTATGG GGGCAGAAAC TCACATAGTC TCACAGACTT GTAATCAGGA TGCCAGAATA 
TTAGCCATGA CCCCAGAATC TATATATTCT AATCCATCTA TTCAAGGTTG TAAGACTGTT 
TTCTATTCTA GTTGTAATGA TGCCATGGAA ATGACCAAAT GTCTCTCAAA TATGAGAGAG 
GAGAAAAATT TGCTAAAGCA TGACAGTAAT TATGCTAAAA TGTATTGCAA TCCAGATGCT 
ATGTCTTCTC TCACAGAGAA AACTATTTAT TCCGGAGAGG AGAACATGGA CATTACCAAG 
AGTCATACAG TTGCAATAGA TAATCAAATT TTTAAACAAG ATCAATCAAA TGTGCAAATA 
GCAGCTGCAC CAACACCCGA AAAAGAAATG ATGCTCCAAA ATCTTATGAC CACATCAGAA 
GATGGGAAAA TGAATGTAAA TTGTAACTCA GTTCCTCATG TATCTAAGGA AAGAATACAG 
CAGAGCCTGT CAAATCCTTT GTCTATTTCA TTGACTGATA GAAAGACTGA ACTCTTATCA 
GGTGAAAATA TGGATTTGAC TGAAAGTCAC ACAAGTAACT TAGGAAGTCA GGTTCCTCTT 
GCAGCTTATA ATCTAGCACC GGAGAGTACC AGTGAATCTC ACTCTCAGAG CAAAAGCTCT 
TCAGATGAAT GTGAAGAAAT TACCAAAAGT CGTAATGAAC CATTTCAGCG ATCAGACATA 
ATAGCCAAAA ACAGCTTAAC CGACACCTGG AACAAAGACA AAGATTGGGT TTTGAAGATT 
TTGCCCTACC TTGATAAAGA TTCTCCTCAG TCAGCTGATT GTAATCAGGA GATAGCAACA 
AGCCATAATA TAGTCTACTG TGGTGGAGTT CTTGATAAAC AAATAACTAA TAGAAATACA 
GTATCATGGG AACAATCTTT GTTTTCTACC ACAAAGCCAT TATTTTCATC AGGACAGTTC 
TCTATGAAAA ATCATGATAC TGCTATAAGT AGTCATACAG TGAAATCTGT ACTAGGCCAG 
AATTCTAAAC TGGCTGAGCC ACTGAGGAAA AGTTTAAGCA ATCCCACACC TGACTATTGC 
CATGACAAGA TGATTATATG TTCAGAGGAA GAGCAAAATA TGGATCTAAC AAAGAGCCAC 
ACTGTCGTCA TTGGATTTGG TCCTTCTGAA CTACAAGAAC TTGGTAAAAC TAATTTAGAA 
CACACTACTG GCCAGCTAAC AACAATGAAC AGACAGATAG CTGTAAAAGT TGAAAAATGT 
GGTAAAAGTC CCATAGAAAA AAGTGGAGTG CTTAAATCTA ACTGTATTAT GGATGTGTTA 
GAGGACGAAA GTGTACAGAA ACCTAAATTT CCAAAGGAAA AGCAAAATGT CAAAATTTGG 
GGAAGGAAAA GTGTTGGTGG ACCAAAAATT GATAAGACTA TTGTATTTTC AGAAGACGAT 
AAGAATGATA TGGATATCAC TAAGAGTTAT ACAATAGAAA TAAACCATAG ACCTTTATTA 
GAGAAACGTG ATTGTCATTT GGTGCCATTG GCAGGAACTT CTGAAACTAT TTTATATACA 
TGTAGGCAGG ATGACATGGA GATCACTAGA AGTCACACAA CTGCCTTAGA ATGTAAAACT 
GTCTCACCAG ATGAAATAAC TACTAGGCCT ATGGACAAAA CTGTAGTGTT TGTAGATAAT 
CATGTTGAAC TAGAAATGAC AGAGTCCCAT ACTGTTTTCA TTGACTACCA AGAAAAGGAA 
AGAACAGACA GACCTAACTT TGAACTATCC CAAAGGAAAA GCCTAGGAAC ACCAACAGTG 
ATATGTACTC CTACTGAGGA GAGTGTTTTC TTTCCAGGAA ATGGTGAAAG TGACCGTCTA 
GTAGCAAATG ACAGCCAGCT AACCCCTCTG GAGGAATGGT CTAATAATAG GGGCCCTGTA 
GAGGTAGCTG ATAACATGGA ATTGTCTAAA TCAGCCACTT GCAAAAACAT CAAAGATGTA 
CAAAGTCCTG GATTTCTGAA TGAACCTCTA TCAAGCAAAA GTCAGAGAAG AAAAAGCCTT 
AAGCTAAAAA ATGACAAGAC CATTGTATTT TCAGAGAATC ATAAAAATGA TATGGATATT 
ACCCAGAGTT GTATGGTGGA AATAGATAAC GAAAGTGCCC TGGAGGATAA AGAGGACTTC 
CATTTGGCAG GGGCTTCTAA AACTATTTTG TATTCATGTG GGCAGGATGA CATGGAGATC 
ACTAGGAGTC ACACAACTGC CTTAGAATGT AAAACTCTCC TGCCAAATGA AATAGCTATT 
AGGCCCATGG ACAAAACCGT ATTGTTCACA GATAATTACA GTGATCTGGA AGTCACCGAT 
TCCCATACTG TTTTCATTGA CTGTCAAGCC ACAGAGAAAA TACTTGAAGA AAACCCTAAA 
TTTGGAATAG GAAAAGGAAA AAACTTGGGT GTTTCCTTTC CTAAGGATAA TAGCTGTGTT 
CAAGAAATCG CTGAAAAACA AGCACTGGCT GTAGGAAACA AAATAGTTCT TCACACCGAG 
CAAAAGCAAC AACTCTTTGC TGCTACTAAT AGAACTACTA ATGAAATCAT CAAATTTCAT 
AGTGCTGCTA TGGATGAAAA GGTCATAGGG AAAGTTGTAG ACCAGGCCTG TACATTGGAA 
AAAGCGCAAG TTGAAAGCTG TCAGTTAAAT AATAGAGATA GAAGAAATGT GGACTTTACA 
AGTAGTCATG CAACTGCTGT TTGTGGATCC AGTGATAATT ATTCCTGTTT ACCAAATGTT 
ATTTCCTGTA CTGATAATTT GGAGGGTAGT GCCATGCTCT TATGTGATAA AGATGAGGAA 
AAAGCCAATT ATTGCCCAGT GCAAAATGAT CTTGCTTATG CAAATGATTT TGCCAGTGAA 
TATTACTTGG AATCTGAGGG ACAGCCTCTC TCTGCTCCTT GTCCTTTGTT AGAGAAGGAA 
GAAGTTATTC AAACCAGTAC CAAAGGACAG TTAGACTGTG TTATAACACT GCACAAAGAT 
CAAGATCTGA TTAAGGATCC ACGAAATCTA TTGGCTAATC AAACTTTAGT ATATAGTCAA 
GATCTGGGGG AGATGACTAA ACTTAATTCA AAGCGAGTAT CTTTTAAGCT TCCAAAGGAT 
CAAATGAAAG TCTATGTTGA TGACATTTAT GTTATTCCTC AGCCTCATTT CTCAACCGAC 
CAACCTCCAT TACCTAAAAA AGGACAGAGT AGTATCAATA AAGAAGAAGT AATACTGTCT 
AAAGCTGGAA ATAAGAGTTT AAATATTATA GAAAATTCCT CTGCACCCAT ATGTGAAAAC 
AAGCCCAAAA TACTCAATAG TGAGGAATGG TTTGCTGCAG CCTGTAAAAA AGAACTGAAG 
GAAAATATTC AAACAACTAA CTATAATACA GCTCTAGATT TCCACAGTAA CTCAGACGTA 
ACTAAGCAAG TCATTCAAAC TCATGTCAAT GCTGGAGAAG CACCAGATCC TGTAATTACA 
TCTAATGTTC CATGTTTTCA TAGTATCAAA CCAAATCTGA ATAATTTGAA TGGAAAAACT 
GGAGAGTTTT TAGCCTTTCA AACTGTTCAT CTACCACCCC TTCCAGAGCA ATTACTTGAA 
TTAGGAAATA AGGCACACAA TGATATGCAT ATAGTGCAAG CTACAGAAAT ACATAATATT 
AACATAATCT CCAGCAATGC TAAAGATAGT AGAGATGAGG AAAATAAAAA GTCTCATAAT 
GGAGCTGAAA CCACCTCTCT ACCGCCAAAG ACAGTTTTTA AAGATAAAGT AAGGAGATGT 
TCTTTGGGAA TCTTTTTGCC TAGATTGCCC AACAAGAGAA ATTGTAGTGT CACTGGTATT 
GATGACCTGG AACAGATTCC AGCAGACACA ACTGATATAA ATCACTTAGA AACTCAGCCG 
GTCTCTAGCA AAGATTCAGG CATTGGATCT GTTGCAGGTA AACTGAACCT AAGTCCTTCT 
CAATATATAA ATGAGGAAAA TCTTCCTGTA TATCCTGATG AGATCAATTC TTCAGACTCT 
ATTAACATAG AAACTGAGGA AAAGGCCTTG ATTGAGACAT ACCAAAAAGA GATTTCACCA 
TATGAAAATA AAATGGGAAA AACTTGCAAT AGCCAAAAAA GAACGTGGGT ACAAGAAGAA 
GAAGATATTC ATAAGGAGAA AAAAATCAGA AAAAATGAGA TTAAGTTTAG TGATACGACA 
CAAGATCGGG AGATTTTTGA TCACCATACT GAAGAGGATA TAGATAAAAG TGCTAACAGT 
GTATTGATAA AAAACCTGAG CAGGACCCCA TCTAGTTGCA GCAGCTCTCT GGATTCAATC 
AAGGCTGATG GGACCTCTCT GGACTTCAGC ACTTACCGCA GTAGTCAAAT GGAATCACAG 
TTTCTCAGAG ATACTATTTG TGAAGAGAGC TTGAGGGAGA AACTCCAAGA TGGGAGAATA 
ACAATAAGGG AGTTCTTTAT ACTTCTCCAG GTCCACATCT TGATACAGAA ACCCCGACAG 
AGCAATCTCC CAGGCAATTT TACTGTAAAC ACACCACCTA CTCCAGAAGA CCTGATGTTA 
AGTCAATATG TTTACCGACC CAAGATACAG ATTTATAGAG AAGATTGTGA GGCTCGTCGC 
CAAAAGATTG AAGAATTAAA GCTTTCTGCA TCGAACCAAG ATAAGCTGTT GGTTGATATA 
AATAAGAACC TGTGGGAAAA AATGAGACAC TGCTCTGACA AAGAGCTGAA GGCCTTTGGA 
ATTTATCTTA ACAAAATAAA GTCATGTTTT ACCAAGATGA CTAAAGTCTT CACTCACCAA 
GGAAAAGTGG CTCTGTATGG CAAGCTGGTG CAGTCAGCTC AGAATGAGAG GGAGAAACTT 
CAAATAAAGA TAGATGAGAT GGATAAAATA CTTAAGAAGA TCGATAACTG CCTCACTGAG 
ATGGAAACAG AAACTAAGAA TTTGGAGGAT GAAGAGAAAA ACAATCCTGT GGAAGAATGG 
GATTCTGAAA TGAGAGCTGC AGAAAAAGAA TTGGAACAGC TGAAAACTGA AGAAGAGGAG 
CTTCAAAGAA ATCTCTTAGA ACTGGAGGTA CAAAAAGAGC AGACCCTTGC TCAAATAGAC 
TTTATGCAAA AACAAAGAAA TAGAACTGAA GAGCTACTGG ATCAGTTGAG CTTGTCTGAG 
TGGGATGTCG TTGAGTGGAG TGATGATCAA GCTGTATTCA CCTTTGTTTA TGACACGATA 
CAACTCACCA TCACCTTTGA AGAGTCAGTT GTTGGTTTCC CTTTCCTGGA CAAGCGTTAT 
AGGAAGATTG TTGATGTCAA TTTTCAATCT CTGTTAGATG AGGATCAAGC TCCTCCTTCC 
TCCCTTTTAG TTCATAAGCT TATTTTCCAG TACGTTGAAG AAAAGGAATC CTGGAAGAAG 
ACATGTACAA CCCAGCATCA GTTACCCAAG ATGCTTGAAG AATTCTCACT GGTAGTGCAC 
CATTGCAGAC TCCTTGGAGA GGAGATTGAG TATTTAAAGA GATGGGGACC AAATTATAAC 
CTAATGAACA TAGATATTAA TAATAATGAA TTGAGACTTT TATTCTCTAG CTCCGCAGCA 
TTTGCAAAGT TTGAAATAAC TTTGTTTCTC TCAGCCTATT ATCCATCTGT ACCATTACCT 
TCCACCATTC AGAATCACGT TGGGAACACT AGCCAAGATG ATATTGCTAC CATTCTATCT 
AAAGTGCCAC TGGAGAACAA CTACCTGAAG AATGTAGTCA AGCAAATTTA CCAAGATCTG 
TTTCAGGACT GCCATTTCTA CCACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

D40/KNL1/CASC5 and autosomal recessive primary microcephaly.
Congenital anomalies57(6)191-196(2017 Nov)
Takimoto M

A sequential multi-target Mps1 phosphorylation cascade promotes spindle checkpoint signaling.
eLife6(2017 Jan)
Ji Z,Gao H,Jia L,Li B,Yu H

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KNL1 cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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