Knl1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Knl1
Entrez Gene ID	76464
Full Name	kinetochore scaffold 1
Synonyms	2310043D08Rik,5730505K17Rik,Casc5
General protein information	Preferred Names kinetochore scaffold 1 Names kinetochore scaffold 1 protein CASC5 cancer susceptibility candidate 5 kinetochore-null protein 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 E5

mRNA and Protein(s)

mRNA	Protein	Name
XM_006500376.2	XP_006500439.1	protein CASC5 isoform X1
XM_006500377.4	XP_006500440.1	kinetochore scaffold 1 isoform X1
XM_030252192.1	XP_030108052.1	kinetochore scaffold 1 isoform X2
NM_029617.3	NP_083893.2	kinetochore scaffold 1 isoform 1
XM_006500377.3	XP_006500440.1	protein CASC5 isoform X1
NM_029617.2	NP_083893.2	kinetochore scaffold 1
XM_006500376.3	XP_006500439.1	kinetochore scaffold 1 isoform X1
NM_001362732.1	NP_001349661.1	kinetochore scaffold 1 isoform 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	CASC5	NP_733468.3
Pan troglodytes (chimpanzee)	CASC5	XP_510312.3
Canis lupus familiaris (dog)	CASC5	XP_005638312.1
Bos taurus (cattle)	CASC5	XP_002690867.3
Mus musculus (house mouse)	Casc5	NP_083893.2
Rattus norvegicus (Norway rat)	Casc5	NP_001164065.1
Gallus gallus (chicken)	CASC5	XP_420938.4

Gene Ontology
Bibliography

Related articles in PubMed

Structural analysis reveals features of the spindle checkpoint kinase Bub1-kinetochore subunit Knl1 interaction.
Krenn V, Wehenkel A, Li X, Santaguida S, Musacchio A
The Journal of cell biology196(4)451-67(2012 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Knl1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Knl1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu84153	NM_001362732.1 Latest version!	Mus musculus kinetochore scaffold 1 (Knl1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu07220	XM_006500376.3 Latest version!	Mus musculus kinetochore scaffold 1 (Knl1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu07220	XM_006500377.4 Latest version!	Mus musculus kinetochore scaffold 1 (Knl1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu88036	XM_030252192.1 Latest version!	Mus musculus kinetochore scaffold 1 (Knl1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu07220	NM_029617.3 Latest version!	Mus musculus kinetochore scaffold 1 (Knl1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
View Old Accession Versions >>
OMu07220	XM_006500377.3	Mus musculus cancer susceptibility candidate 5 (Casc5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu07220	NM_029617.2	Mus musculus kinetochore scaffold 1 (Knl1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu07220	XM_006500376.2	Mus musculus cancer susceptibility candidate 5 (Casc5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Knl1 ( NM_001362732.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001349661.1 Mus musculus kinetochore scaffold 1 (Knl1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu84153
Clone ID Related Accession (Same CDS sequence)	NM_001362732.1
Accession Version	NM_001362732.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4839bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Mus musculus(house mouse)
Product	kinetochore scaffold 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK009772.1, BC080815.1 and AL772264.8. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC080815.1, AK033132.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849383, SAMN00849384 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATGGGG TATATTCAGA AGCTAATGAA GAGAATGACA ATACACAGAG ACCTGTTAGA 
AGACAGCATT CCTCAATTTT GAAACCCCCA AGGAGTCCTC TTCAGGACCT CAAATGTGGA 
AATCAAACAA ACCAGGAACC TAATCCTCCA AGAAAAAGGA AAAGCTCTCG TCGAGTCAGC 
TTTGCAGATA CTATAAAAGT ATTCCAGACA GAGTCTCACA TGAAAACAGA GAGAAATTCA 
GAAATTTCAG GTATGAACAC TTTGCTTTGT GCTCCAATTC AGACTCAGAT GCAACAAAAG 
GAGTTCTCAA TTACAGACTG CAACCATGAA AGGAAACATG CAAATGATCA GACAGTCATC 
TTTTCAGATG AAAACCAGAT GGATCTGACA GCAAGTCACA CTGTAATGAT TACCAAAGGT 
CTTTCAGACT GTACCAAAAA TGAAAATTCT ACAAAAATAG ATACCACGTC CTTTCTGGAG 
AATTTAAAGC ATCATGCTGC AAATTCAAGA ATTAAAAAGG ATTTAGCATG TTCCACAGTT 
TCCTTAAGTC AAAACATTTT CTCAGAAAAG ATCAATTCTG ACAACTTCAT AAAAAGATTG 
AAGACAGGAA AACATATTTC GTCTTCTACA GAGCTTGATA AGGAAAATGC TGAGATACCT 
GTTTATTCCA AAGACTCAAA CAGTGCCTCT TCAACATATC AAATGCATGC ATCTCTTGGT 
GTAGATGAAA ATAGTAGTAA TAGGACTAGA ATCTTTAGAG AACAAGATGA TGGTATGAAT 
TTGACCCAAT GTCATACAGC CTGTATTAAG ACTTGGATTC CTCCATCCAC GGAGGCCAAA 
ATAGGAGAAT TCAAAGGTGA TAAAACAATT TATGGCAATG AGTGTATGGA GTTGACAACT 
AACTATACGA TACAGGTTTT GTCATCAGAA AATAATTTAT CTGAAAGAGA AACTCAAACT 
CAAAATGGTA TGAATGTTAC AACAGTTGAT GGAGCTACAG CTCCAGCTCC AGAGAAGAAA 
ACAGCCCTTA AGGATAAACT AAATGCTGCT TTTCAAGGCT CTTTCCCAAA CCCTGAAAAT 
AAAATTCATA TTATCAAATG TCATCCTATA GAGTCAGAAA CTCATACAGT CACACAGATT 
TCTAGTCAAA GTGCCAGCAC ATTGGCTGTA ACTTCAAAAT CTATATGTTC TAGTCCAGCT 
ATTGAGGGGT ATAAGACAAT TTTTCATTCT AGTAGCAATG ATGCAATGGA ACTGACTAAG 
TGTCTATCAG CTATGGAAGA GGAAAAAAAA TTGCTGAAGG CTGATGATAA GTATTCTAAA 
ATATGTACCA ATCCAGATGC TGGCCCACTC AGAGAGAAAA CTATTTATTT AGAAGAGGAT 
AGCATGGACA TTACCAAAAG TCACACCGTT GCAATAGATA ATAAAATATT TAAACATGAT 
CAAGAAAACA TTAAAAAAGA AATAATAGCC ATACCAATAT TTGAAAAGGA AATGGTGCTC 
CGAAATCTTA TGCCTATGTC AAAAGATGAG AAAAGGGATG TAAATTATAT TTCAGTTCCT 
CAAGTATCTA AGGAAAGCTT ACAACGGAGT CAGACAAACA CTTTATCTGT TTCATTGACT 
GACAAAAAGA TGGAATTCTT GGCAGATGAA GATATGGATC TGACTAAAAG TCACACAACT 
AAGTTAAGTC AAGTTATTCC TACAACTTTT GACTTAGCAT CAAAGAATGT CACCAAATCA 
TACTCTCACA GCAAAAGCCC TTTAAATGAG TGGGAAAGCC TGGATAAACA AGTAGTGCTA 
GGCCAGCATT CTAAACTTCC TTTACCACAG AGGAAAGATA GAGATGATCC TGACTGTTCT 
CATCATAAGA TAATGTATTC AGAGGAGTTA CAAACTATGG ATCTAACCAA GAGCCACACT 
ATTGTAATTG GCTTTGGTCC TTCTGAAGTA CAAGAACATA GTAAAATTAA TTTAGAACAC 
AAAAACAGCC AACTTACAGC AGAAAGCATA CAAACAGCTG TGAACGTTCC TGCAGCAAAC 
TCTAGAGTAG TAACAACTAA TGATATGGAC ATGTTAAAGG ACAGAAGTAC ACATAAGCCA 
GAATTGCTGA AGGAAAAGCA AAACATCAAA ATCTATGGAA GAAAAAGTAT TGGTAGACTG 
AAAATTGATA AAACTATATT ATTTTCAGAG GGTAATGAGG GTGATATGGA TATCACCAAG 
AGTTGCACAG TCAAAATAAA TCATAGATCT TTATTAGATA AGCATGATTC TCATTTAGTA 
TCTTTGGCAG GAACTTCTAA AACCATTTTG CATGCACGTG GTCAGGTTGA AATGGAAATC 
AATAGAAGTC ACACAACTCC TTTAGAATGT AAAATTATCT CGCCCAGTGA TATAACTCCT 
GGTGATCTGG ATAAGACTAT GATGTCTATA GATGATCATG AGGAACTAGA CATGACCAAG 
TCCCATACTG TTTTCATTGA CTACCAAGCA GAGGCAAAAG GTGTACTTCC AGACAGACTT 
GACTTTCAAT TATCCAAAAA GGAAAGCCTA CAGAAGCCGA AAGTGACATC TCTGGCTGAA 
GAGATTTACA TTTCAAAAAA TAGTGAAAGC AACCATCTAC CAGCAAAAGG CAGCCAGCTA 
ACAATATTAG AGGAAGGGTC TAATAGCGGG CTTGGAGAGG AAACTAATGA TGCACAAAAG 
CCTGGATTTC TGAATGAACT TCTGTCAGGC AAAACTCAGA GAAGAAAAAG CCTTAGCCTC 
AAAAACAAGA GTATTACATT TCCAGAGAAT GATAAAAGTT ATAGAGAGAT CCCTCAGAGT 
TCTGCAGTAG AAATAAATAA TGAAACTAGG CTGGAAGACA GAAAGGGCTT CAGTTTTGTG 
CCATTGGCAG GAACTTCCAA ACCAGTTTTG TCTGCATATG GGCCCGAGGA CATGGAGATT 
TCTATAGGTC AAACCACTGC TTCAGAATAT AAAACTGTCC CACCAGAAGA AATAACTACT 
ATACCTATGG ATAAAACTGT AATGTTTGTA GATAATTTCG GTGATCTGGA TGTCACCAGA 
TCCCATACGG TTTTCATCGA TTGTCAAGCC AAAGAAAAGG TACTTGATGA ATATACTAAC 
TTGGGAATAC AGAAAACAAA AACTTTGAGT GGTTCTGAAG GTGATACCCA TATTCAAGAA 
ATCACAAAAA ACCCAGCAGC TCAACACAAA CACCATATGA CAACTGTTAT TCCTTCTAGT 
ACAGTAGTTA GTGATCAAAG TTCAATGAAA ATCAAGTTTC ACAAAGCTGA TAGAGATGAG 
GAGGTCAAAG GAAAGGAAGT GGAAGCCAAT ATGCTGAAAC AAACTAAACC TGAAAGCTGT 
CTGTTAAATA TAACAGATGG AAAAAATGTG GATTTTACAA GCAGTTATAC AGCTGATGTT 
TGTAGATCCA GTGACAAGTA TTCCTCTTTA CCAAATATTT CTTCTTCTGA TAATTCGGGT 
GGTAATACTA TGTCCTTGTG TGATAAAAAT AAGGAGAAAG CTTATAATTG CCAAGTGCCA 
AATGAATTTA CTTATGCAGC TATCTTACCA AGTACATATC ACATGGATTC TAAAAAACTG 
TCTGTCTTTC CACCTTGTCC TTCAAAAGAA GTCACTCAAA CTGAAAGTGC CATAGCGCTG 
CTTAAAGATG AAGATCCGGT AGAAGAGCCT CTGGGGGAGA TGGCTACATT TAACTCAAAG 
CATGTATCTC TTAACCTGGC AAAGGATCAA ACAGAGGCCT TTGTTGATGT TAGTGTTGCT 
TCTCAGCCTC ATCTCTCAGC CCAACAAAGT CCATCAACTC AAAAAGGGCA GGATGTTGCC 
CGTAGAGATG AAGGAATATT AGCTAAAGCT GGGAAGAAGG CTTTACCTTT TCTTCTAGAA 
AATGTCGCTG CATCCACATG GGAAAATGAG TCCAAAATAC CCACTAATGT GGAACACTTT 
GCTGTAACCT ATGAAAAAGA ACTGAGTATA AGTATTCAAA CAGATAAATG TAATACAAAT 
GTACAGAGCC CTAGTAACTC GGCCCTGACA ACACAAGTCA TTCAGACGCA TGCAAATGCT 
GAAGGAGCAC TAGACTTTTT AGTTCCATCT ACTGTTTCAT GTTTTAGTAG TACCAAACCA 
AGTCTGAGTA ATTTGAATAG AAAAACTGAG GAGGTCTTAG ACTTTCAGAC TGTTAATTTA 
TTACCTCCTG CCGAACAGTT ACTTGAAGAA GGAAGTCAGG CACATAGTAT GAGTATAGTA 
CAAGCTACAG AAATATATAG ACTAGGTTCC AGGAATGATA GAGATGAAGA AAGTAAAACT 
TTCTGTAATG AGGCTGAAAC TACATCTGTA CCACTAAAAA CAGCTGTTAA AGACAAAACA 
AGAAGATGTT CATTGGGGAT ATTTTTACCC AAATTACCAA GCAAGAGAAG TTGTAGTATC 
ACTGGTGTTG ATGACCTGGA ACAGATTCTA GCAGATGCAG CTGACTTAAC TCAACTAGAA 
ACTCAGCCAG TCTGTAGTAA AGATCCAGGC ATTGGATCTG TTGCAGCCAA ACTGAATTTG 
AGTCCATCTC AGTTTATAAA TGAGGAAAAT CTCCCTGTAT ATCCTGGTGA GATACTTTCA 
TCAGATTCTG TTAGCCTGGA CATTGAGGAA AGTGTTCTGA TTGACACATC TCAGAGAGAG 
AGTTTACCGT CTGAAAACAA AACCGAAAAC TGCAGGGCCC AAAAAAGAAC CCGGGTTGAA 
GAAAATGATG TTACAAATGA GAAAAAAATC AGAACACATG ACAGTGCACA AGATCAAGAG 
GTAAGCTGTC TTGAGCCAAA AAATGTTCTT GAATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001349661.1
CDS	459..5297
Translation	MDGVYSEANEENDNTQRPVRRQHSSILKPPRSPLQDLKCGNQTNQEPNPPRKRKSSRRVSFADTIKVFQTESHMKTERNSEISGMNTLLCAPIQTQMQQKEFSITDCNHERKHANDQTVIFSDENQMDLTASHTVMITKGLSDCTKNENSTKIDTTSFLENLKHHAANSRIKKDLACSTVSLSQNIFSEKINSDNFIKRLKTGKHISSSTELDKENAEIPVYSKDSNSASSTYQMHASLGVDENSSNRTRIFREQDDGMNLTQCHTACIKTWIPPSTEAKIGEFKGDKTIYGNECMELTTNYTIQVLSSENNLSERETQTQNGMNVTTVDGATAPAPEKKTALKDKLNAAFQGSFPNPENKIHIIKCHPIESETHTVTQISSQSASTLAVTSKSICSSPAIEGYKTIFHSSSNDAMELTKCLSAMEEEKKLLKADDKYSKICTNPDAGPLREKTIYLEEDSMDITKSHTVAIDNKIFKHDQENIKKEIIAIPIFEKEMVLRNLMPMSKDEKRDVNYISVPQVSKESLQRSQTNTLSVSLTDKKMEFLADEDMDLTKSHTTKLSQVIPTTFDLASKNVTKSYSHSKSPLNEWESLDKQVVLGQHSKLPLPQRKDRDDPDCSHHKIMYSEELQTMDLTKSHTIVIGFGPSEVQEHSKINLEHKNSQLTAESIQTAVNVPAANSRVVTTNDMDMLKDRSTHKPELLKEKQNIKIYGRKSIGRLKIDKTILFSEGNEGDMDITKSCTVKINHRSLLDKHDSHLVSLAGTSKTILHARGQVEMEINRSHTTPLECKIISPSDITPGDLDKTMMSIDDHEELDMTKSHTVFIDYQAEAKGVLPDRLDFQLSKKESLQKPKVTSLAEEIYISKNSESNHLPAKGSQLTILEEGSNSGLGEETNDAQKPGFLNELLSGKTQRRKSLSLKNKSITFPENDKSYREIPQSSAVEINNETRLEDRKGFSFVPLAGTSKPVLSAYGPEDMEISIGQTTASEYKTVPPEEITTIPMDKTVMFVDNFGDLDVTRSHTVFIDCQAKEKVLDEYTNLGIQKTKTLSGSEGDTHIQEITKNPAAQHKHHMTTVIPSSTVVSDQSSMKIKFHKADRDEEVKGKEVEANMLKQTKPESCLLNITDGKNVDFTSSYTADVCRSSDKYSSLPNISSSDNSGGNTMSLCDKNKEKAYNCQVPNEFTYAAILPSTYHMDSKKLSVFPPCPSKEVTQTESAIALLKDEDPVEEPLGEMATFNSKHVSLNLAKDQTEAFVDVSVASQPHLSAQQSPSTQKGQDVARRDEGILAKAGKKALPFLLENVAASTWENESKIPTNVEHFAVTYEKELSISIQTDKCNTNVQSPSNSALTTQVIQTHANAEGALDFLVPSTVSCFSSTKPSLSNLNRKTEEVLDFQTVNLLPPAEQLLEEGSQAHSMSIVQATEIYRLGSRNDRDEESKTFCNEAETTSVPLKTAVKDKTRRCSLGIFLPKLPSKRSCSITGVDDLEQILADAADLTQLETQPVCSKDPGIGSVAAKLNLSPSQFINEENLPVYPGEILSSDSVSLDIEESVLIDTSQRESLPSENKTENCRAQKRTRVEENDVTNEKKIRTHDSAQDQEVSCLEPKNVLEF

Target ORF information:

RefSeq Version	NM_001362732.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinetochore scaffold 1 (Knl1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001362732.1

ORF Insert Sequence:

ATGGATGGGG TATATTCAGA AGCTAATGAA GAGAATGACA ATACACAGAG ACCTGTTAGA 
AGACAGCATT CCTCAATTTT GAAACCCCCA AGGAGTCCTC TTCAGGACCT CAAATGTGGA 
AATCAAACAA ACCAGGAACC TAATCCTCCA AGAAAAAGGA AAAGCTCTCG TCGAGTCAGC 
TTTGCAGATA CTATAAAAGT ATTCCAGACA GAGTCTCACA TGAAAACAGA GAGAAATTCA 
GAAATTTCAG GTATGAACAC TTTGCTTTGT GCTCCAATTC AGACTCAGAT GCAACAAAAG 
GAGTTCTCAA TTACAGACTG CAACCATGAA AGGAAACATG CAAATGATCA GACAGTCATC 
TTTTCAGATG AAAACCAGAT GGATCTGACA GCAAGTCACA CTGTAATGAT TACCAAAGGT 
CTTTCAGACT GTACCAAAAA TGAAAATTCT ACAAAAATAG ATACCACGTC CTTTCTGGAG 
AATTTAAAGC ATCATGCTGC AAATTCAAGA ATTAAAAAGG ATTTAGCATG TTCCACAGTT 
TCCTTAAGTC AAAACATTTT CTCAGAAAAG ATCAATTCTG ACAACTTCAT AAAAAGATTG 
AAGACAGGAA AACATATTTC GTCTTCTACA GAGCTTGATA AGGAAAATGC TGAGATACCT 
GTTTATTCCA AAGACTCAAA CAGTGCCTCT TCAACATATC AAATGCATGC ATCTCTTGGT 
GTAGATGAAA ATAGTAGTAA TAGGACTAGA ATCTTTAGAG AACAAGATGA TGGTATGAAT 
TTGACCCAAT GTCATACAGC CTGTATTAAG ACTTGGATTC CTCCATCCAC GGAGGCCAAA 
ATAGGAGAAT TCAAAGGTGA TAAAACAATT TATGGCAATG AGTGTATGGA GTTGACAACT 
AACTATACGA TACAGGTTTT GTCATCAGAA AATAATTTAT CTGAAAGAGA AACTCAAACT 
CAAAATGGTA TGAATGTTAC AACAGTTGAT GGAGCTACAG CTCCAGCTCC AGAGAAGAAA 
ACAGCCCTTA AGGATAAACT AAATGCTGCT TTTCAAGGCT CTTTCCCAAA CCCTGAAAAT 
AAAATTCATA TTATCAAATG TCATCCTATA GAGTCAGAAA CTCATACAGT CACACAGATT 
TCTAGTCAAA GTGCCAGCAC ATTGGCTGTA ACTTCAAAAT CTATATGTTC TAGTCCAGCT 
ATTGAGGGGT ATAAGACAAT TTTTCATTCT AGTAGCAATG ATGCAATGGA ACTGACTAAG 
TGTCTATCAG CTATGGAAGA GGAAAAAAAA TTGCTGAAGG CTGATGATAA GTATTCTAAA 
ATATGTACCA ATCCAGATGC TGGCCCACTC AGAGAGAAAA CTATTTATTT AGAAGAGGAT 
AGCATGGACA TTACCAAAAG TCACACCGTT GCAATAGATA ATAAAATATT TAAACATGAT 
CAAGAAAACA TTAAAAAAGA AATAATAGCC ATACCAATAT TTGAAAAGGA AATGGTGCTC 
CGAAATCTTA TGCCTATGTC AAAAGATGAG AAAAGGGATG TAAATTATAT TTCAGTTCCT 
CAAGTATCTA AGGAAAGCTT ACAACGGAGT CAGACAAACA CTTTATCTGT TTCATTGACT 
GACAAAAAGA TGGAATTCTT GGCAGATGAA GATATGGATC TGACTAAAAG TCACACAACT 
AAGTTAAGTC AAGTTATTCC TACAACTTTT GACTTAGCAT CAAAGAATGT CACCAAATCA 
TACTCTCACA GCAAAAGCCC TTTAAATGAG TGGGAAAGCC TGGATAAACA AGTAGTGCTA 
GGCCAGCATT CTAAACTTCC TTTACCACAG AGGAAAGATA GAGATGATCC TGACTGTTCT 
CATCATAAGA TAATGTATTC AGAGGAGTTA CAAACTATGG ATCTAACCAA GAGCCACACT 
ATTGTAATTG GCTTTGGTCC TTCTGAAGTA CAAGAACATA GTAAAATTAA TTTAGAACAC 
AAAAACAGCC AACTTACAGC AGAAAGCATA CAAACAGCTG TGAACGTTCC TGCAGCAAAC 
TCTAGAGTAG TAACAACTAA TGATATGGAC ATGTTAAAGG ACAGAAGTAC ACATAAGCCA 
GAATTGCTGA AGGAAAAGCA AAACATCAAA ATCTATGGAA GAAAAAGTAT TGGTAGACTG 
AAAATTGATA AAACTATATT ATTTTCAGAG GGTAATGAGG GTGATATGGA TATCACCAAG 
AGTTGCACAG TCAAAATAAA TCATAGATCT TTATTAGATA AGCATGATTC TCATTTAGTA 
TCTTTGGCAG GAACTTCTAA AACCATTTTG CATGCACGTG GTCAGGTTGA AATGGAAATC 
AATAGAAGTC ACACAACTCC TTTAGAATGT AAAATTATCT CGCCCAGTGA TATAACTCCT 
GGTGATCTGG ATAAGACTAT GATGTCTATA GATGATCATG AGGAACTAGA CATGACCAAG 
TCCCATACTG TTTTCATTGA CTACCAAGCA GAGGCAAAAG GTGTACTTCC AGACAGACTT 
GACTTTCAAT TATCCAAAAA GGAAAGCCTA CAGAAGCCGA AAGTGACATC TCTGGCTGAA 
GAGATTTACA TTTCAAAAAA TAGTGAAAGC AACCATCTAC CAGCAAAAGG CAGCCAGCTA 
ACAATATTAG AGGAAGGGTC TAATAGCGGG CTTGGAGAGG AAACTAATGA TGCACAAAAG 
CCTGGATTTC TGAATGAACT TCTGTCAGGC AAAACTCAGA GAAGAAAAAG CCTTAGCCTC 
AAAAACAAGA GTATTACATT TCCAGAGAAT GATAAAAGTT ATAGAGAGAT CCCTCAGAGT 
TCTGCAGTAG AAATAAATAA TGAAACTAGG CTGGAAGACA GAAAGGGCTT CAGTTTTGTG 
CCATTGGCAG GAACTTCCAA ACCAGTTTTG TCTGCATATG GGCCCGAGGA CATGGAGATT 
TCTATAGGTC AAACCACTGC TTCAGAATAT AAAACTGTCC CACCAGAAGA AATAACTACT 
ATACCTATGG ATAAAACTGT AATGTTTGTA GATAATTTCG GTGATCTGGA TGTCACCAGA 
TCCCATACGG TTTTCATCGA TTGTCAAGCC AAAGAAAAGG TACTTGATGA ATATACTAAC 
TTGGGAATAC AGAAAACAAA AACTTTGAGT GGTTCTGAAG GTGATACCCA TATTCAAGAA 
ATCACAAAAA ACCCAGCAGC TCAACACAAA CACCATATGA CAACTGTTAT TCCTTCTAGT 
ACAGTAGTTA GTGATCAAAG TTCAATGAAA ATCAAGTTTC ACAAAGCTGA TAGAGATGAG 
GAGGTCAAAG GAAAGGAAGT GGAAGCCAAT ATGCTGAAAC AAACTAAACC TGAAAGCTGT 
CTGTTAAATA TAACAGATGG AAAAAATGTG GATTTTACAA GCAGTTATAC AGCTGATGTT 
TGTAGATCCA GTGACAAGTA TTCCTCTTTA CCAAATATTT CTTCTTCTGA TAATTCGGGT 
GGTAATACTA TGTCCTTGTG TGATAAAAAT AAGGAGAAAG CTTATAATTG CCAAGTGCCA 
AATGAATTTA CTTATGCAGC TATCTTACCA AGTACATATC ACATGGATTC TAAAAAACTG 
TCTGTCTTTC CACCTTGTCC TTCAAAAGAA GTCACTCAAA CTGAAAGTGC CATAGCGCTG 
CTTAAAGATG AAGATCCGGT AGAAGAGCCT CTGGGGGAGA TGGCTACATT TAACTCAAAG 
CATGTATCTC TTAACCTGGC AAAGGATCAA ACAGAGGCCT TTGTTGATGT TAGTGTTGCT 
TCTCAGCCTC ATCTCTCAGC CCAACAAAGT CCATCAACTC AAAAAGGGCA GGATGTTGCC 
CGTAGAGATG AAGGAATATT AGCTAAAGCT GGGAAGAAGG CTTTACCTTT TCTTCTAGAA 
AATGTCGCTG CATCCACATG GGAAAATGAG TCCAAAATAC CCACTAATGT GGAACACTTT 
GCTGTAACCT ATGAAAAAGA ACTGAGTATA AGTATTCAAA CAGATAAATG TAATACAAAT 
GTACAGAGCC CTAGTAACTC GGCCCTGACA ACACAAGTCA TTCAGACGCA TGCAAATGCT 
GAAGGAGCAC TAGACTTTTT AGTTCCATCT ACTGTTTCAT GTTTTAGTAG TACCAAACCA 
AGTCTGAGTA ATTTGAATAG AAAAACTGAG GAGGTCTTAG ACTTTCAGAC TGTTAATTTA 
TTACCTCCTG CCGAACAGTT ACTTGAAGAA GGAAGTCAGG CACATAGTAT GAGTATAGTA 
CAAGCTACAG AAATATATAG ACTAGGTTCC AGGAATGATA GAGATGAAGA AAGTAAAACT 
TTCTGTAATG AGGCTGAAAC TACATCTGTA CCACTAAAAA CAGCTGTTAA AGACAAAACA 
AGAAGATGTT CATTGGGGAT ATTTTTACCC AAATTACCAA GCAAGAGAAG TTGTAGTATC 
ACTGGTGTTG ATGACCTGGA ACAGATTCTA GCAGATGCAG CTGACTTAAC TCAACTAGAA 
ACTCAGCCAG TCTGTAGTAA AGATCCAGGC ATTGGATCTG TTGCAGCCAA ACTGAATTTG 
AGTCCATCTC AGTTTATAAA TGAGGAAAAT CTCCCTGTAT ATCCTGGTGA GATACTTTCA 
TCAGATTCTG TTAGCCTGGA CATTGAGGAA AGTGTTCTGA TTGACACATC TCAGAGAGAG 
AGTTTACCGT CTGAAAACAA AACCGAAAAC TGCAGGGCCC AAAAAAGAAC CCGGGTTGAA 
GAAAATGATG TTACAAATGA GAAAAAAATC AGAACACATG ACAGTGCACA AGATCAAGAG 
GTAAGCTGTC TTGAGCCAAA AAATGTTCTT GAATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Knl1 ( XM_006500377.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006500440.1 Mus musculus kinetochore scaffold 1 (Knl1), transcript variant X2, mRNA.

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CloneID	OMu07220
Clone ID Related Accession (Same CDS sequence)	XM_006500376.2 , XM_006500377.4 , NM_029617.3 , XM_006500377.3 , NM_029617.2 , XM_006500376.3
Accession Version	XM_006500377.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kinetochore scaffold 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006500377.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGGGG TATATTCAGA AGCTAATGAA GAGAATGACA ATACACAGAG ACCTGTTAGA 
AGACAGCATT CCTCAATTTT GAAACCCCCA AGGAGTCCTC TTCAGGACCT CAAATGTGGA 
AATCAAACAA ACCAGGAACC TAATCCTCCA AGAAAAAGGA AAAGCTCTCG TCGAGTCAGC 
TTTGCAGATA CTATAAAAGT ATTCCAGACA GAGTCTCACA TGAAAACAGA GAGAAATTCA 
GAAATTTCAG GTATGAACAC TTTGCTTTGT GCTCCAATTC AGACTCAGAT GCAACAAAAG 
GAGTTCTCAA TTACAGACTG CAACCATGAA AGGAAACATG CAAATGATCA GACAGTCATC 
TTTTCAGATG AAAACCAGAT GGATCTGACA GCAAGTCACA CTGTAATGAT TACCAAAGGT 
CTTTCAGACT GTACCAAAAA TGAAAATTCT ACAAAAATAG ATACCACGTC CTTTCTGGAG 
AATTTAAAGC ATCATGCTGC AAATTCAAGA ATTAAAAAGG ATTTAGCATG TTCCACAGTT 
TCCTTAAGTC AAAACATTTT CTCAGAAAAG ATCAATTCTG ACAACTTCAT AAAAAGATTG 
AAGACAGGAA AACATATTTC GTCTTCTACA GAGCTTGATA AGGAAAATGC TGAGATACCT 
GTTTATTCCA AAGACTCAAA CAGTGCCTCT TCAACATATC AAATGCATGC ATCTCTTGGT 
GTAGATGAAA ATAGTAGTAA TAGGACTAGA ATCTTTAGAG AACAAGATGA TGGTATGAAT 
TTGACCCAAT GTCATACAGC CTGTATTAAG ACTTGGATTC CTCCATCCAC GGAGGCCAAA 
ATAGGAGAAT TCAAAGGTGA TAAAACAATT TATGGCAATG AGTGTATGGA GTTGACAACT 
AACTATACGA TACAGGTTTT GTCATCAGAA AATAATTTAT CTGAAAGAGA AACTCAAACT 
CAAAATGGTA TGAATGTTAC AACAGTTGAT GGAGCTACAG CTCCAGCTCC AGAGAAGAAA 
ACAGCCCTTA AGGATAAACT AAATGCTGCT TTTCAAGGCT CTTTCCCAAA CCCTGAAAAT 
AAAATTCATA TTATCAAATG TCATCCTATA GAGTCAGAAA CTCATACAGT CACACAGATT 
TCTAGTCAAA GTGCCAGCAC ATTGGCTGTA ACTTCAAAAT CTATATGTTC TAGTCCAGCT 
ATTGAGGGGT ATAAGACAAT TTTTCATTCT AGTAGCAATG ATGCAATGGA ACTGACTAAG 
TGTCTATCAG CTATGGAAGA GGAAAAAAAA TTGCTGAAGG CTGATGATAA GTATTCTAAA 
ATATGTACCA ATCCAGATGC TGGCCCACTC AGAGAGAAAA CTATTTATTT AGAAGAGGAT 
AGCATGGACA TTACCAAAAG TCACACCGTT GCAATAGATA ATAAAATATT TAAACATGAT 
CAAGAAAACA TTAAAAAAGA AATAATAGCC ATACCAATAT TTGAAAAGGA AATGGTGCTC 
CGAAATCTTA TGCCTATGTC AAAAGATGAG AAAAGGGATG TAAATTATAT TTCAGTTCCT 
CAAGTATCTA AGGAAAGCTT ACAACGGAGT CAGACAAACA CTTTATCTGT TTCATTGACT 
GACAAAAAGA TGGAATTCTT GGCAGATGAA GATATGGATC TGACTAAAAG TCACACAACT 
AAGTTAAGTC AAGTTATTCC TACAACTTTT GACTTAGCAT CAAAGAATGT CACCAAATCA 
TACTCTCACA GCAAAAGCCC TTTAAATGAG TGGGAAAGCC TGGATAAACA AGTAGTGCTA 
GGCCAGCATT CTAAACTTCC TTTACCACAG AGGAAAGATA GAGATGATCC TGACTGTTCT 
CATCATAAGA TAATGTATTC AGAGGAGTTA CAAACTATGG ATCTAACCAA GAGCCACACT 
ATTGTAATTG GCTTTGGTCC TTCTGAAGTA CAAGAACATA GTAAAATTAA TTTAGAACAC 
AAAAACAGCC AACTTACAGC AGAAAGCATA CAAACAGCTG TGAACGTTCC TGCAGCAAAC 
TCTAGAGTAG TAACAACTAA TGATATGGAC ATGTTAAAGG ACAGAAGTAC ACATAAGCCA 
GAATTGCTGA AGGAAAAGCA AAACATCAAA ATCTATGGAA GAAAAAGTAT TGGTAGACTG 
AAAATTGATA AAACTATATT ATTTTCAGAG GGTAATGAGG GTGATATGGA TATCACCAAG 
AGTTGCACAG TCAAAATAAA TCATAGATCT TTATTAGATA AGCATGATTC TCATTTAGTA 
TCTTTGGCAG GAACTTCTAA AACCATTTTG CATGCACGTG GTCAGGTTGA AATGGAAATC 
AATAGAAGTC ACACAACTCC TTTAGAATGT AAAATTATCT CGCCCAGTGA TATAACTCCT 
GGTGATCTGG ATAAGACTAT GATGTCTATA GATGATCATG AGGAACTAGA CATGACCAAG 
TCCCATACTG TTTTCATTGA CTACCAAGCA GAGGCAAAAG GTGTACTTCC AGACAGACTT 
GACTTTCAAT TATCCAAAAA GGAAAGCCTA CAGAAGCCGA AAGTGACATC TCTGGCTGAA 
GAGATTTACA TTTCAAAAAA TAGTGAAAGC AACCATCTAC CAGCAAAAGG CAGCCAGCTA 
ACAATATTAG AGGAAGGGTC TAATAGCGGG CTTGGAGAGG AAACTAATGA TGCACAAAAG 
CCTGGATTTC TGAATGAACT TCTGTCAGGC AAAACTCAGA GAAGAAAAAG CCTTAGCCTC 
AAAAACAAGA GTATTACATT TCCAGAGAAT GATAAAAGTT ATAGAGAGAT CCCTCAGAGT 
TCTGCAGTAG AAATAAATAA TGAAACTAGG CTGGAAGACA GAAAGGGCTT CAGTTTTGTG 
CCATTGGCAG GAACTTCCAA ACCAGTTTTG TCTGCATATG GGCCCGAGGA CATGGAGATT 
TCTATAGGTC AAACCACTGC TTCAGAATAT AAAACTGTCC CACCAGAAGA AATAACTACT 
ATACCTATGG ATAAAACTGT AATGTTTGTA GATAATTTCG GTGATCTGGA TGTCACCAGA 
TCCCATACGG TTTTCATCGA TTGTCAAGCC AAAGAAAAGG TACTTGATGA ATATACTAAC 
TTGGGAATAC AGAAAACAAA AACTTTGAGT GGTTCTGAAG GTGATACCCA TATTCAAGAA 
ATCACAAAAA ACCCAGCAGC TCAACACAAA CACCATATGA CAACTGTTAT TCCTTCTAGT 
ACAGTAGTTA GTGATCAAAG TTCAATGAAA ATCAAGTTTC ACAAAGCTGA TAGAGATGAG 
GAGGTCAAAG GAAAGGAAGT GGAAGCCAAT ATGCTGAAAC AAACTAAACC TGAAAGCTGT 
CTGTTAAATA TAACAGATGG AAAAAATGTG GATTTTACAA GCAGTTATAC AGCTGATGTT 
TGTAGATCCA GTGACAAGTA TTCCTCTTTA CCAAATATTT CTTCTTCTGA TAATTCGGGT 
GGTAATACTA TGTCCTTGTG TGATAAAAAT AAGGAGAAAG CTTATAATTG CCAAGTGCCA 
AATGAATTTA CTTATGCAGC TATCTTACCA AGTACATATC ACATGGATTC TAAAAAACTG 
TCTGTCTTTC CACCTTGTCC TTCAAAAGAA GTCACTCAAA CTGAAAGTGC CATAGCGCTG 
CTTAAAGATG AAGATCCGGT AGAAGAGCCT CTGGGGGAGA TGGCTACATT TAACTCAAAG 
CATGTATCTC TTAACCTGGC AAAGGATCAA ACAGAGGCCT TTGTTGATGT TAGTGTTGCT 
TCTCAGCCTC ATCTCTCAGC CCAACAAAGT CCATCAACTC AAAAAGGGCA GGATGTTGCC 
CGTAGAGATG AAGGAATATT AGCTAAAGCT GGGAAGAAGG CTTTACCTTT TCTTCTAGAA 
AATGTCGCTG CATCCACATG GGAAAATGAG TCCAAAATAC CCACTAATGT GGAACACTTT 
GCTGTAACCT ATGAAAAAGA ACTGAGTATA AGTATTCAAA CAGATAAATG TAATACAAAT 
GTACAGAGCC CTAGTAACTC GGCCCTGACA ACACAAGTCA TTCAGACGCA TGCAAATGCT 
GAAGGAGCAC TAGACTTTTT AGTTCCATCT ACTGTTTCAT GTTTTAGTAG TACCAAACCA 
AGTCTGAGTA ATTTGAATAG AAAAACTGAG GAGGTCTTAG ACTTTCAGAC TGTTAATTTA 
TTACCTCCTG CCGAACAGTT ACTTGAAGAA GGAAGTCAGG CACATAGTAT GAGTATAGTA 
CAAGCTACAG AAATATATAG ACTAGGTTCC AGGAATGATA GAGATGAAGA AAGTAAAACT 
TTCTGTAATG AGGCTGAAAC TACATCTGTA CCACTAAAAA CAGCTGTTAA AGACAAAACA 
AGAAGATGTT CATTGGGGAT ATTTTTACCC AAATTACCAA GCAAGAGAAG TTGTAGTATC 
ACTGGTGTTG ATGACCTGGA ACAGATTCTA GCAGATGCAG CTGACTTAAC TCAACTAGAA 
ACTCAGCCAG TCTGTAGTAA AGATCCAGGC ATTGGATCTG TTGCAGCCAA ACTGAATTTG 
AGTCCATCTC AGTTTATAAA TGAGGAAAAT CTCCCTGTAT ATCCTGGTGA GATACTTTCA 
TCAGATTCTG TTAGCCTGGA CATTGAGGAA AGTGTTCTGA TTGACACATC TCAGAGAGAG 
AGTTTACCGT CTGAAAACAA AACCGAAAAC TGCAGGGCCC AAAAAAGAAC CCGGGTTGAA 
GAAAATGATG TTACAAATGA GAAAAAAATC AGAACACATG ACAGTGCACA AGATCAAGAG 
ATTTTTGATA ATCATATGGA AGAGGATATC AATAAAAATG TCAACAGTGT ATTGCTGAAA 
AGCCTGAGCA GGACACCGTC TAGTTGCAGC AGCTCTCTGG ATTCAATCAA GTCTGATGGA 
CTCTCTCTGG ATGTCAGCAC ACAGCGTAAT AGCCAAATGG AATCACAGTT TCTTGGTGAC 
ACTATCTCTG AAGAGAGTTT GAAGGAGAAA CTCAAGGATG GACAAATTAC AATAAAGGAG 
TTCTTCATAC TTCTTCAGGT CCACATCTTA ATACAGAAAC CTAGGCAGAG CACTCTCCCA 
GCCAAATTTA CTATAAACAC ATTACCTACC ACAGAAGATC TGATGTTGAG ACAATATGTT 
TATGGACCCA GGATACAGAT TTATAAAGAA GACTGTGAGC TTCTTCGACA AAAGATTGAC 
GAATTAAAGA TATCTGCATT GAACCAAAAT AAACTATTGG CGGATGTAAA TAGGAACTTG 
TGGGAAAAAG TGAAAGACTA CTCTGATGAA GAGCTGAAGA ACTATGGGAT TTACCTTAAC 
AAAATCAAAT CACGTTATAC CAAGATGACA AAAGTCTTCA ATCACCAAGG AAAAGTGGCT 
CTGTATAACA AATTGGTGCA TTCAGCTGAG AATGAGAAGA ACAAACTTCA GATAAAAATA 
AATGAGATGG ACACTATCCT CAAGAAGATC AATAACTGTC TCGCTGAAGT GGAAACAGAA 
ACTAAGAATT TGGAGAATGA AGAGAAAAAT GATGCCATGG AAGAATGGGA TTCTGAAATG 
AGAGATGCAG AGAAAGAACT GGAACAGCTG AAAACTGAAG AAGAGGAGCT TCAGAGAAAG 
TTCTTAGAAG TAGAGACACA GAAAACGCAG ACCCTTGCTC AGATAGAATT TATAAAAGAA 
CAAACAACTA AAACTGAAGA ATTACTGGAT CAATTGAGTT TGTCTGAGTG GGATGTCATT 
GAGTGGAGTG ATGATCAAGC TGTATTTACC TTTGTCTATG ATTCAATAGA ACTCATCATC 
ACCTTTGGAG AGCCACTAGT TGGTCTCCCT TTTTTGGACA AGGCTTGCAG GAAGATTAAT 
GCACTCAGTT TTCAGTCTCT GTTAGATGAA GATAAAGCTC CTCCCTCCTC CCTTTTAGTT 
CACAAGCTTA TTTTTCAGTA CATTGAGGAA CAGGAATCCT GGAAGAAGAA ATGCACAGCA 
CAGCATCAGG TGCCCCAGAT GCTTCAAGAA CTCTCACTGG TAGTGAACCA TTGTAGGCTT 
CTTGGAGAGG AGATCGAATT TTTAAAGCGA TGGGGGCCAA ATTATAGCCT AATGCATATA 
AATGTGAATA ATACTGAATT GAGGCTTTTA TTCTCCAGTT GTGCAGCATT TGCAAAGTTT 
GAGATAACTT TGTCTCCCTC GGCCCATTAT CCCTTGGTCC CGTTACCGTT CACCATTCAC 
AATCACATTG GGAAGACTGG CCATGATGAA ATTGCTGCAA TTATCTCTAA AGTGCCCTTA 
GAGGAAAACT ACCTGAAGAA TGTAGTCAAG CAAATTTACC AAGATCTACT TAAGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006500440.1
CDS	101..6460
Translation	MDGVYSEANEENDNTQRPVRRQHSSILKPPRSPLQDLKCGNQTNQEPNPPRKRKSSRRVSFADTIKVFQTESHMKTERNSEISGMNTLLCAPIQTQMQQKEFSITDCNHERKHANDQTVIFSDENQMDLTASHTVMITKGLSDCTKNENSTKIDTTSFLENLKHHAANSRIKKDLACSTVSLSQNIFSEKINSDNFIKRLKTGKHISSSTELDKENAEIPVYSKDSNSASSTYQMHASLGVDENSSNRTRIFREQDDGMNLTQCHTACIKTWIPPSTEAKIGEFKGDKTIYGNECMELTTNYTIQVLSSENNLSERETQTQNGMNVTTVDGATAPAPEKKTALKDKLNAAFQGSFPNPENKIHIIKCHPIESETHTVTQISSQSASTLAVTSKSICSSPAIEGYKTIFHSSSNDAMELTKCLSAMEEEKKLLKADDKYSKICTNPDAGPLREKTIYLEEDSMDITKSHTVAIDNKIFKHDQENIKKEIIAIPIFEKEMVLRNLMPMSKDEKRDVNYISVPQVSKESLQRSQTNTLSVSLTDKKMEFLADEDMDLTKSHTTKLSQVIPTTFDLASKNVTKSYSHSKSPLNEWESLDKQVVLGQHSKLPLPQRKDRDDPDCSHHKIMYSEELQTMDLTKSHTIVIGFGPSEVQEHSKINLEHKNSQLTAESIQTAVNVPAANSRVVTTNDMDMLKDRSTHKPELLKEKQNIKIYGRKSIGRLKIDKTILFSEGNEGDMDITKSCTVKINHRSLLDKHDSHLVSLAGTSKTILHARGQVEMEINRSHTTPLECKIISPSDITPGDLDKTMMSIDDHEELDMTKSHTVFIDYQAEAKGVLPDRLDFQLSKKESLQKPKVTSLAEEIYISKNSESNHLPAKGSQLTILEEGSNSGLGEETNDAQKPGFLNELLSGKTQRRKSLSLKNKSITFPENDKSYREIPQSSAVEINNETRLEDRKGFSFVPLAGTSKPVLSAYGPEDMEISIGQTTASEYKTVPPEEITTIPMDKTVMFVDNFGDLDVTRSHTVFIDCQAKEKVLDEYTNLGIQKTKTLSGSEGDTHIQEITKNPAAQHKHHMTTVIPSSTVVSDQSSMKIKFHKADRDEEVKGKEVEANMLKQTKPESCLLNITDGKNVDFTSSYTADVCRSSDKYSSLPNISSSDNSGGNTMSLCDKNKEKAYNCQVPNEFTYAAILPSTYHMDSKKLSVFPPCPSKEVTQTESAIALLKDEDPVEEPLGEMATFNSKHVSLNLAKDQTEAFVDVSVASQPHLSAQQSPSTQKGQDVARRDEGILAKAGKKALPFLLENVAASTWENESKIPTNVEHFAVTYEKELSISIQTDKCNTNVQSPSNSALTTQVIQTHANAEGALDFLVPSTVSCFSSTKPSLSNLNRKTEEVLDFQTVNLLPPAEQLLEEGSQAHSMSIVQATEIYRLGSRNDRDEESKTFCNEAETTSVPLKTAVKDKTRRCSLGIFLPKLPSKRSCSITGVDDLEQILADAADLTQLETQPVCSKDPGIGSVAAKLNLSPSQFINEENLPVYPGEILSSDSVSLDIEESVLIDTSQRESLPSENKTENCRAQKRTRVEENDVTNEKKIRTHDSAQDQEIFDNHMEEDINKNVNSVLLKSLSRTPSSCSSSLDSIKSDGLSLDVSTQRNSQMESQFLGDTISEESLKEKLKDGQITIKEFFILLQVHILIQKPRQSTLPAKFTINTLPTTEDLMLRQYVYGPRIQIYKEDCELLRQKIDELKISALNQNKLLADVNRNLWEKVKDYSDEELKNYGIYLNKIKSRYTKMTKVFNHQGKVALYNKLVHSAENEKNKLQIKINEMDTILKKINNCLAEVETETKNLENEEKNDAMEEWDSEMRDAEKELEQLKTEEEELQRKFLEVETQKTQTLAQIEFIKEQTTKTEELLDQLSLSEWDVIEWSDDQAVFTFVYDSIELIITFGEPLVGLPFLDKACRKINALSFQSLLDEDKAPPSSLLVHKLIFQYIEEQESWKKKCTAQHQVPQMLQELSLVVNHCRLLGEEIEFLKRWGPNYSLMHINVNNTELRLLFSSCAAFAKFEITLSPSAHYPLVPLPFTIHNHIGKTGHDEIAAIISKVPLEENYLKNVVKQIYQDLLKD

Target ORF information:

RefSeq Version	XM_006500377.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinetochore scaffold 1 (Knl1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006500377.4

ORF Insert Sequence:

ATGGATGGGG TATATTCAGA AGCTAATGAA GAGAATGACA ATACACAGAG ACCTGTTAGA 
AGACAGCATT CCTCAATTTT GAAACCCCCA AGGAGTCCTC TTCAGGACCT CAAATGTGGA 
AATCAAACAA ACCAGGAACC TAATCCTCCA AGAAAAAGGA AAAGCTCTCG TCGAGTCAGC 
TTTGCAGATA CTATAAAAGT ATTCCAGACA GAGTCTCACA TGAAAACAGA GAGAAATTCA 
GAAATTTCAG GTATGAACAC TTTGCTTTGT GCTCCAATTC AGACTCAGAT GCAACAAAAG 
GAGTTCTCAA TTACAGACTG CAACCATGAA AGGAAACATG CAAATGATCA GACAGTCATC 
TTTTCAGATG AAAACCAGAT GGATCTGACA GCAAGTCACA CTGTAATGAT TACCAAAGGT 
CTTTCAGACT GTACCAAAAA TGAAAATTCT ACAAAAATAG ATACCACGTC CTTTCTGGAG 
AATTTAAAGC ATCATGCTGC AAATTCAAGA ATTAAAAAGG ATTTAGCATG TTCCACAGTT 
TCCTTAAGTC AAAACATTTT CTCAGAAAAG ATCAATTCTG ACAACTTCAT AAAAAGATTG 
AAGACAGGAA AACATATTTC GTCTTCTACA GAGCTTGATA AGGAAAATGC TGAGATACCT 
GTTTATTCCA AAGACTCAAA CAGTGCCTCT TCAACATATC AAATGCATGC ATCTCTTGGT 
GTAGATGAAA ATAGTAGTAA TAGGACTAGA ATCTTTAGAG AACAAGATGA TGGTATGAAT 
TTGACCCAAT GTCATACAGC CTGTATTAAG ACTTGGATTC CTCCATCCAC GGAGGCCAAA 
ATAGGAGAAT TCAAAGGTGA TAAAACAATT TATGGCAATG AGTGTATGGA GTTGACAACT 
AACTATACGA TACAGGTTTT GTCATCAGAA AATAATTTAT CTGAAAGAGA AACTCAAACT 
CAAAATGGTA TGAATGTTAC AACAGTTGAT GGAGCTACAG CTCCAGCTCC AGAGAAGAAA 
ACAGCCCTTA AGGATAAACT AAATGCTGCT TTTCAAGGCT CTTTCCCAAA CCCTGAAAAT 
AAAATTCATA TTATCAAATG TCATCCTATA GAGTCAGAAA CTCATACAGT CACACAGATT 
TCTAGTCAAA GTGCCAGCAC ATTGGCTGTA ACTTCAAAAT CTATATGTTC TAGTCCAGCT 
ATTGAGGGGT ATAAGACAAT TTTTCATTCT AGTAGCAATG ATGCAATGGA ACTGACTAAG 
TGTCTATCAG CTATGGAAGA GGAAAAAAAA TTGCTGAAGG CTGATGATAA GTATTCTAAA 
ATATGTACCA ATCCAGATGC TGGCCCACTC AGAGAGAAAA CTATTTATTT AGAAGAGGAT 
AGCATGGACA TTACCAAAAG TCACACCGTT GCAATAGATA ATAAAATATT TAAACATGAT 
CAAGAAAACA TTAAAAAAGA AATAATAGCC ATACCAATAT TTGAAAAGGA AATGGTGCTC 
CGAAATCTTA TGCCTATGTC AAAAGATGAG AAAAGGGATG TAAATTATAT TTCAGTTCCT 
CAAGTATCTA AGGAAAGCTT ACAACGGAGT CAGACAAACA CTTTATCTGT TTCATTGACT 
GACAAAAAGA TGGAATTCTT GGCAGATGAA GATATGGATC TGACTAAAAG TCACACAACT 
AAGTTAAGTC AAGTTATTCC TACAACTTTT GACTTAGCAT CAAAGAATGT CACCAAATCA 
TACTCTCACA GCAAAAGCCC TTTAAATGAG TGGGAAAGCC TGGATAAACA AGTAGTGCTA 
GGCCAGCATT CTAAACTTCC TTTACCACAG AGGAAAGATA GAGATGATCC TGACTGTTCT 
CATCATAAGA TAATGTATTC AGAGGAGTTA CAAACTATGG ATCTAACCAA GAGCCACACT 
ATTGTAATTG GCTTTGGTCC TTCTGAAGTA CAAGAACATA GTAAAATTAA TTTAGAACAC 
AAAAACAGCC AACTTACAGC AGAAAGCATA CAAACAGCTG TGAACGTTCC TGCAGCAAAC 
TCTAGAGTAG TAACAACTAA TGATATGGAC ATGTTAAAGG ACAGAAGTAC ACATAAGCCA 
GAATTGCTGA AGGAAAAGCA AAACATCAAA ATCTATGGAA GAAAAAGTAT TGGTAGACTG 
AAAATTGATA AAACTATATT ATTTTCAGAG GGTAATGAGG GTGATATGGA TATCACCAAG 
AGTTGCACAG TCAAAATAAA TCATAGATCT TTATTAGATA AGCATGATTC TCATTTAGTA 
TCTTTGGCAG GAACTTCTAA AACCATTTTG CATGCACGTG GTCAGGTTGA AATGGAAATC 
AATAGAAGTC ACACAACTCC TTTAGAATGT AAAATTATCT CGCCCAGTGA TATAACTCCT 
GGTGATCTGG ATAAGACTAT GATGTCTATA GATGATCATG AGGAACTAGA CATGACCAAG 
TCCCATACTG TTTTCATTGA CTACCAAGCA GAGGCAAAAG GTGTACTTCC AGACAGACTT 
GACTTTCAAT TATCCAAAAA GGAAAGCCTA CAGAAGCCGA AAGTGACATC TCTGGCTGAA 
GAGATTTACA TTTCAAAAAA TAGTGAAAGC AACCATCTAC CAGCAAAAGG CAGCCAGCTA 
ACAATATTAG AGGAAGGGTC TAATAGCGGG CTTGGAGAGG AAACTAATGA TGCACAAAAG 
CCTGGATTTC TGAATGAACT TCTGTCAGGC AAAACTCAGA GAAGAAAAAG CCTTAGCCTC 
AAAAACAAGA GTATTACATT TCCAGAGAAT GATAAAAGTT ATAGAGAGAT CCCTCAGAGT 
TCTGCAGTAG AAATAAATAA TGAAACTAGG CTGGAAGACA GAAAGGGCTT CAGTTTTGTG 
CCATTGGCAG GAACTTCCAA ACCAGTTTTG TCTGCATATG GGCCCGAGGA CATGGAGATT 
TCTATAGGTC AAACCACTGC TTCAGAATAT AAAACTGTCC CACCAGAAGA AATAACTACT 
ATACCTATGG ATAAAACTGT AATGTTTGTA GATAATTTCG GTGATCTGGA TGTCACCAGA 
TCCCATACGG TTTTCATCGA TTGTCAAGCC AAAGAAAAGG TACTTGATGA ATATACTAAC 
TTGGGAATAC AGAAAACAAA AACTTTGAGT GGTTCTGAAG GTGATACCCA TATTCAAGAA 
ATCACAAAAA ACCCAGCAGC TCAACACAAA CACCATATGA CAACTGTTAT TCCTTCTAGT 
ACAGTAGTTA GTGATCAAAG TTCAATGAAA ATCAAGTTTC ACAAAGCTGA TAGAGATGAG 
GAGGTCAAAG GAAAGGAAGT GGAAGCCAAT ATGCTGAAAC AAACTAAACC TGAAAGCTGT 
CTGTTAAATA TAACAGATGG AAAAAATGTG GATTTTACAA GCAGTTATAC AGCTGATGTT 
TGTAGATCCA GTGACAAGTA TTCCTCTTTA CCAAATATTT CTTCTTCTGA TAATTCGGGT 
GGTAATACTA TGTCCTTGTG TGATAAAAAT AAGGAGAAAG CTTATAATTG CCAAGTGCCA 
AATGAATTTA CTTATGCAGC TATCTTACCA AGTACATATC ACATGGATTC TAAAAAACTG 
TCTGTCTTTC CACCTTGTCC TTCAAAAGAA GTCACTCAAA CTGAAAGTGC CATAGCGCTG 
CTTAAAGATG AAGATCCGGT AGAAGAGCCT CTGGGGGAGA TGGCTACATT TAACTCAAAG 
CATGTATCTC TTAACCTGGC AAAGGATCAA ACAGAGGCCT TTGTTGATGT TAGTGTTGCT 
TCTCAGCCTC ATCTCTCAGC CCAACAAAGT CCATCAACTC AAAAAGGGCA GGATGTTGCC 
CGTAGAGATG AAGGAATATT AGCTAAAGCT GGGAAGAAGG CTTTACCTTT TCTTCTAGAA 
AATGTCGCTG CATCCACATG GGAAAATGAG TCCAAAATAC CCACTAATGT GGAACACTTT 
GCTGTAACCT ATGAAAAAGA ACTGAGTATA AGTATTCAAA CAGATAAATG TAATACAAAT 
GTACAGAGCC CTAGTAACTC GGCCCTGACA ACACAAGTCA TTCAGACGCA TGCAAATGCT 
GAAGGAGCAC TAGACTTTTT AGTTCCATCT ACTGTTTCAT GTTTTAGTAG TACCAAACCA 
AGTCTGAGTA ATTTGAATAG AAAAACTGAG GAGGTCTTAG ACTTTCAGAC TGTTAATTTA 
TTACCTCCTG CCGAACAGTT ACTTGAAGAA GGAAGTCAGG CACATAGTAT GAGTATAGTA 
CAAGCTACAG AAATATATAG ACTAGGTTCC AGGAATGATA GAGATGAAGA AAGTAAAACT 
TTCTGTAATG AGGCTGAAAC TACATCTGTA CCACTAAAAA CAGCTGTTAA AGACAAAACA 
AGAAGATGTT CATTGGGGAT ATTTTTACCC AAATTACCAA GCAAGAGAAG TTGTAGTATC 
ACTGGTGTTG ATGACCTGGA ACAGATTCTA GCAGATGCAG CTGACTTAAC TCAACTAGAA 
ACTCAGCCAG TCTGTAGTAA AGATCCAGGC ATTGGATCTG TTGCAGCCAA ACTGAATTTG 
AGTCCATCTC AGTTTATAAA TGAGGAAAAT CTCCCTGTAT ATCCTGGTGA GATACTTTCA 
TCAGATTCTG TTAGCCTGGA CATTGAGGAA AGTGTTCTGA TTGACACATC TCAGAGAGAG 
AGTTTACCGT CTGAAAACAA AACCGAAAAC TGCAGGGCCC AAAAAAGAAC CCGGGTTGAA 
GAAAATGATG TTACAAATGA GAAAAAAATC AGAACACATG ACAGTGCACA AGATCAAGAG 
ATTTTTGATA ATCATATGGA AGAGGATATC AATAAAAATG TCAACAGTGT ATTGCTGAAA 
AGCCTGAGCA GGACACCGTC TAGTTGCAGC AGCTCTCTGG ATTCAATCAA GTCTGATGGA 
CTCTCTCTGG ATGTCAGCAC ACAGCGTAAT AGCCAAATGG AATCACAGTT TCTTGGTGAC 
ACTATCTCTG AAGAGAGTTT GAAGGAGAAA CTCAAGGATG GACAAATTAC AATAAAGGAG 
TTCTTCATAC TTCTTCAGGT CCACATCTTA ATACAGAAAC CTAGGCAGAG CACTCTCCCA 
GCCAAATTTA CTATAAACAC ATTACCTACC ACAGAAGATC TGATGTTGAG ACAATATGTT 
TATGGACCCA GGATACAGAT TTATAAAGAA GACTGTGAGC TTCTTCGACA AAAGATTGAC 
GAATTAAAGA TATCTGCATT GAACCAAAAT AAACTATTGG CGGATGTAAA TAGGAACTTG 
TGGGAAAAAG TGAAAGACTA CTCTGATGAA GAGCTGAAGA ACTATGGGAT TTACCTTAAC 
AAAATCAAAT CACGTTATAC CAAGATGACA AAAGTCTTCA ATCACCAAGG AAAAGTGGCT 
CTGTATAACA AATTGGTGCA TTCAGCTGAG AATGAGAAGA ACAAACTTCA GATAAAAATA 
AATGAGATGG ACACTATCCT CAAGAAGATC AATAACTGTC TCGCTGAAGT GGAAACAGAA 
ACTAAGAATT TGGAGAATGA AGAGAAAAAT GATGCCATGG AAGAATGGGA TTCTGAAATG 
AGAGATGCAG AGAAAGAACT GGAACAGCTG AAAACTGAAG AAGAGGAGCT TCAGAGAAAG 
TTCTTAGAAG TAGAGACACA GAAAACGCAG ACCCTTGCTC AGATAGAATT TATAAAAGAA 
CAAACAACTA AAACTGAAGA ATTACTGGAT CAATTGAGTT TGTCTGAGTG GGATGTCATT 
GAGTGGAGTG ATGATCAAGC TGTATTTACC TTTGTCTATG ATTCAATAGA ACTCATCATC 
ACCTTTGGAG AGCCACTAGT TGGTCTCCCT TTTTTGGACA AGGCTTGCAG GAAGATTAAT 
GCACTCAGTT TTCAGTCTCT GTTAGATGAA GATAAAGCTC CTCCCTCCTC CCTTTTAGTT 
CACAAGCTTA TTTTTCAGTA CATTGAGGAA CAGGAATCCT GGAAGAAGAA ATGCACAGCA 
CAGCATCAGG TGCCCCAGAT GCTTCAAGAA CTCTCACTGG TAGTGAACCA TTGTAGGCTT 
CTTGGAGAGG AGATCGAATT TTTAAAGCGA TGGGGGCCAA ATTATAGCCT AATGCATATA 
AATGTGAATA ATACTGAATT GAGGCTTTTA TTCTCCAGTT GTGCAGCATT TGCAAAGTTT 
GAGATAACTT TGTCTCCCTC GGCCCATTAT CCCTTGGTCC CGTTACCGTT CACCATTCAC 
AATCACATTG GGAAGACTGG CCATGATGAA ATTGCTGCAA TTATCTCTAA AGTGCCCTTA 
GAGGAAAACT ACCTGAAGAA TGTAGTCAAG CAAATTTACC AAGATCTACT TAAGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Knl1 ( XM_006500376.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006500439.1 Mus musculus kinetochore scaffold 1 (Knl1), transcript variant X1, mRNA.

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CloneID	OMu07220
Clone ID Related Accession (Same CDS sequence)	XM_006500376.2 , XM_006500377.4 , NM_029617.3 , XM_006500377.3 , NM_029617.2 , XM_006500376.3
Accession Version	XM_006500376.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kinetochore scaffold 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006500376.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGGGG TATATTCAGA AGCTAATGAA GAGAATGACA ATACACAGAG ACCTGTTAGA 
AGACAGCATT CCTCAATTTT GAAACCCCCA AGGAGTCCTC TTCAGGACCT CAAATGTGGA 
AATCAAACAA ACCAGGAACC TAATCCTCCA AGAAAAAGGA AAAGCTCTCG TCGAGTCAGC 
TTTGCAGATA CTATAAAAGT ATTCCAGACA GAGTCTCACA TGAAAACAGA GAGAAATTCA 
GAAATTTCAG GTATGAACAC TTTGCTTTGT GCTCCAATTC AGACTCAGAT GCAACAAAAG 
GAGTTCTCAA TTACAGACTG CAACCATGAA AGGAAACATG CAAATGATCA GACAGTCATC 
TTTTCAGATG AAAACCAGAT GGATCTGACA GCAAGTCACA CTGTAATGAT TACCAAAGGT 
CTTTCAGACT GTACCAAAAA TGAAAATTCT ACAAAAATAG ATACCACGTC CTTTCTGGAG 
AATTTAAAGC ATCATGCTGC AAATTCAAGA ATTAAAAAGG ATTTAGCATG TTCCACAGTT 
TCCTTAAGTC AAAACATTTT CTCAGAAAAG ATCAATTCTG ACAACTTCAT AAAAAGATTG 
AAGACAGGAA AACATATTTC GTCTTCTACA GAGCTTGATA AGGAAAATGC TGAGATACCT 
GTTTATTCCA AAGACTCAAA CAGTGCCTCT TCAACATATC AAATGCATGC ATCTCTTGGT 
GTAGATGAAA ATAGTAGTAA TAGGACTAGA ATCTTTAGAG AACAAGATGA TGGTATGAAT 
TTGACCCAAT GTCATACAGC CTGTATTAAG ACTTGGATTC CTCCATCCAC GGAGGCCAAA 
ATAGGAGAAT TCAAAGGTGA TAAAACAATT TATGGCAATG AGTGTATGGA GTTGACAACT 
AACTATACGA TACAGGTTTT GTCATCAGAA AATAATTTAT CTGAAAGAGA AACTCAAACT 
CAAAATGGTA TGAATGTTAC AACAGTTGAT GGAGCTACAG CTCCAGCTCC AGAGAAGAAA 
ACAGCCCTTA AGGATAAACT AAATGCTGCT TTTCAAGGCT CTTTCCCAAA CCCTGAAAAT 
AAAATTCATA TTATCAAATG TCATCCTATA GAGTCAGAAA CTCATACAGT CACACAGATT 
TCTAGTCAAA GTGCCAGCAC ATTGGCTGTA ACTTCAAAAT CTATATGTTC TAGTCCAGCT 
ATTGAGGGGT ATAAGACAAT TTTTCATTCT AGTAGCAATG ATGCAATGGA ACTGACTAAG 
TGTCTATCAG CTATGGAAGA GGAAAAAAAA TTGCTGAAGG CTGATGATAA GTATTCTAAA 
ATATGTACCA ATCCAGATGC TGGCCCACTC AGAGAGAAAA CTATTTATTT AGAAGAGGAT 
AGCATGGACA TTACCAAAAG TCACACCGTT GCAATAGATA ATAAAATATT TAAACATGAT 
CAAGAAAACA TTAAAAAAGA AATAATAGCC ATACCAATAT TTGAAAAGGA AATGGTGCTC 
CGAAATCTTA TGCCTATGTC AAAAGATGAG AAAAGGGATG TAAATTATAT TTCAGTTCCT 
CAAGTATCTA AGGAAAGCTT ACAACGGAGT CAGACAAACA CTTTATCTGT TTCATTGACT 
GACAAAAAGA TGGAATTCTT GGCAGATGAA GATATGGATC TGACTAAAAG TCACACAACT 
AAGTTAAGTC AAGTTATTCC TACAACTTTT GACTTAGCAT CAAAGAATGT CACCAAATCA 
TACTCTCACA GCAAAAGCCC TTTAAATGAG TGGGAAAGCC TGGATAAACA AGTAGTGCTA 
GGCCAGCATT CTAAACTTCC TTTACCACAG AGGAAAGATA GAGATGATCC TGACTGTTCT 
CATCATAAGA TAATGTATTC AGAGGAGTTA CAAACTATGG ATCTAACCAA GAGCCACACT 
ATTGTAATTG GCTTTGGTCC TTCTGAAGTA CAAGAACATA GTAAAATTAA TTTAGAACAC 
AAAAACAGCC AACTTACAGC AGAAAGCATA CAAACAGCTG TGAACGTTCC TGCAGCAAAC 
TCTAGAGTAG TAACAACTAA TGATATGGAC ATGTTAAAGG ACAGAAGTAC ACATAAGCCA 
GAATTGCTGA AGGAAAAGCA AAACATCAAA ATCTATGGAA GAAAAAGTAT TGGTAGACTG 
AAAATTGATA AAACTATATT ATTTTCAGAG GGTAATGAGG GTGATATGGA TATCACCAAG 
AGTTGCACAG TCAAAATAAA TCATAGATCT TTATTAGATA AGCATGATTC TCATTTAGTA 
TCTTTGGCAG GAACTTCTAA AACCATTTTG CATGCACGTG GTCAGGTTGA AATGGAAATC 
AATAGAAGTC ACACAACTCC TTTAGAATGT AAAATTATCT CGCCCAGTGA TATAACTCCT 
GGTGATCTGG ATAAGACTAT GATGTCTATA GATGATCATG AGGAACTAGA CATGACCAAG 
TCCCATACTG TTTTCATTGA CTACCAAGCA GAGGCAAAAG GTGTACTTCC AGACAGACTT 
GACTTTCAAT TATCCAAAAA GGAAAGCCTA CAGAAGCCGA AAGTGACATC TCTGGCTGAA 
GAGATTTACA TTTCAAAAAA TAGTGAAAGC AACCATCTAC CAGCAAAAGG CAGCCAGCTA 
ACAATATTAG AGGAAGGGTC TAATAGCGGG CTTGGAGAGG AAACTAATGA TGCACAAAAG 
CCTGGATTTC TGAATGAACT TCTGTCAGGC AAAACTCAGA GAAGAAAAAG CCTTAGCCTC 
AAAAACAAGA GTATTACATT TCCAGAGAAT GATAAAAGTT ATAGAGAGAT CCCTCAGAGT 
TCTGCAGTAG AAATAAATAA TGAAACTAGG CTGGAAGACA GAAAGGGCTT CAGTTTTGTG 
CCATTGGCAG GAACTTCCAA ACCAGTTTTG TCTGCATATG GGCCCGAGGA CATGGAGATT 
TCTATAGGTC AAACCACTGC TTCAGAATAT AAAACTGTCC CACCAGAAGA AATAACTACT 
ATACCTATGG ATAAAACTGT AATGTTTGTA GATAATTTCG GTGATCTGGA TGTCACCAGA 
TCCCATACGG TTTTCATCGA TTGTCAAGCC AAAGAAAAGG TACTTGATGA ATATACTAAC 
TTGGGAATAC AGAAAACAAA AACTTTGAGT GGTTCTGAAG GTGATACCCA TATTCAAGAA 
ATCACAAAAA ACCCAGCAGC TCAACACAAA CACCATATGA CAACTGTTAT TCCTTCTAGT 
ACAGTAGTTA GTGATCAAAG TTCAATGAAA ATCAAGTTTC ACAAAGCTGA TAGAGATGAG 
GAGGTCAAAG GAAAGGAAGT GGAAGCCAAT ATGCTGAAAC AAACTAAACC TGAAAGCTGT 
CTGTTAAATA TAACAGATGG AAAAAATGTG GATTTTACAA GCAGTTATAC AGCTGATGTT 
TGTAGATCCA GTGACAAGTA TTCCTCTTTA CCAAATATTT CTTCTTCTGA TAATTCGGGT 
GGTAATACTA TGTCCTTGTG TGATAAAAAT AAGGAGAAAG CTTATAATTG CCAAGTGCCA 
AATGAATTTA CTTATGCAGC TATCTTACCA AGTACATATC ACATGGATTC TAAAAAACTG 
TCTGTCTTTC CACCTTGTCC TTCAAAAGAA GTCACTCAAA CTGAAAGTGC CATAGCGCTG 
CTTAAAGATG AAGATCCGGT AGAAGAGCCT CTGGGGGAGA TGGCTACATT TAACTCAAAG 
CATGTATCTC TTAACCTGGC AAAGGATCAA ACAGAGGCCT TTGTTGATGT TAGTGTTGCT 
TCTCAGCCTC ATCTCTCAGC CCAACAAAGT CCATCAACTC AAAAAGGGCA GGATGTTGCC 
CGTAGAGATG AAGGAATATT AGCTAAAGCT GGGAAGAAGG CTTTACCTTT TCTTCTAGAA 
AATGTCGCTG CATCCACATG GGAAAATGAG TCCAAAATAC CCACTAATGT GGAACACTTT 
GCTGTAACCT ATGAAAAAGA ACTGAGTATA AGTATTCAAA CAGATAAATG TAATACAAAT 
GTACAGAGCC CTAGTAACTC GGCCCTGACA ACACAAGTCA TTCAGACGCA TGCAAATGCT 
GAAGGAGCAC TAGACTTTTT AGTTCCATCT ACTGTTTCAT GTTTTAGTAG TACCAAACCA 
AGTCTGAGTA ATTTGAATAG AAAAACTGAG GAGGTCTTAG ACTTTCAGAC TGTTAATTTA 
TTACCTCCTG CCGAACAGTT ACTTGAAGAA GGAAGTCAGG CACATAGTAT GAGTATAGTA 
CAAGCTACAG AAATATATAG ACTAGGTTCC AGGAATGATA GAGATGAAGA AAGTAAAACT 
TTCTGTAATG AGGCTGAAAC TACATCTGTA CCACTAAAAA CAGCTGTTAA AGACAAAACA 
AGAAGATGTT CATTGGGGAT ATTTTTACCC AAATTACCAA GCAAGAGAAG TTGTAGTATC 
ACTGGTGTTG ATGACCTGGA ACAGATTCTA GCAGATGCAG CTGACTTAAC TCAACTAGAA 
ACTCAGCCAG TCTGTAGTAA AGATCCAGGC ATTGGATCTG TTGCAGCCAA ACTGAATTTG 
AGTCCATCTC AGTTTATAAA TGAGGAAAAT CTCCCTGTAT ATCCTGGTGA GATACTTTCA 
TCAGATTCTG TTAGCCTGGA CATTGAGGAA AGTGTTCTGA TTGACACATC TCAGAGAGAG 
AGTTTACCGT CTGAAAACAA AACCGAAAAC TGCAGGGCCC AAAAAAGAAC CCGGGTTGAA 
GAAAATGATG TTACAAATGA GAAAAAAATC AGAACACATG ACAGTGCACA AGATCAAGAG 
ATTTTTGATA ATCATATGGA AGAGGATATC AATAAAAATG TCAACAGTGT ATTGCTGAAA 
AGCCTGAGCA GGACACCGTC TAGTTGCAGC AGCTCTCTGG ATTCAATCAA GTCTGATGGA 
CTCTCTCTGG ATGTCAGCAC ACAGCGTAAT AGCCAAATGG AATCACAGTT TCTTGGTGAC 
ACTATCTCTG AAGAGAGTTT GAAGGAGAAA CTCAAGGATG GACAAATTAC AATAAAGGAG 
TTCTTCATAC TTCTTCAGGT CCACATCTTA ATACAGAAAC CTAGGCAGAG CACTCTCCCA 
GCCAAATTTA CTATAAACAC ATTACCTACC ACAGAAGATC TGATGTTGAG ACAATATGTT 
TATGGACCCA GGATACAGAT TTATAAAGAA GACTGTGAGC TTCTTCGACA AAAGATTGAC 
GAATTAAAGA TATCTGCATT GAACCAAAAT AAACTATTGG CGGATGTAAA TAGGAACTTG 
TGGGAAAAAG TGAAAGACTA CTCTGATGAA GAGCTGAAGA ACTATGGGAT TTACCTTAAC 
AAAATCAAAT CACGTTATAC CAAGATGACA AAAGTCTTCA ATCACCAAGG AAAAGTGGCT 
CTGTATAACA AATTGGTGCA TTCAGCTGAG AATGAGAAGA ACAAACTTCA GATAAAAATA 
AATGAGATGG ACACTATCCT CAAGAAGATC AATAACTGTC TCGCTGAAGT GGAAACAGAA 
ACTAAGAATT TGGAGAATGA AGAGAAAAAT GATGCCATGG AAGAATGGGA TTCTGAAATG 
AGAGATGCAG AGAAAGAACT GGAACAGCTG AAAACTGAAG AAGAGGAGCT TCAGAGAAAG 
TTCTTAGAAG TAGAGACACA GAAAACGCAG ACCCTTGCTC AGATAGAATT TATAAAAGAA 
CAAACAACTA AAACTGAAGA ATTACTGGAT CAATTGAGTT TGTCTGAGTG GGATGTCATT 
GAGTGGAGTG ATGATCAAGC TGTATTTACC TTTGTCTATG ATTCAATAGA ACTCATCATC 
ACCTTTGGAG AGCCACTAGT TGGTCTCCCT TTTTTGGACA AGGCTTGCAG GAAGATTAAT 
GCACTCAGTT TTCAGTCTCT GTTAGATGAA GATAAAGCTC CTCCCTCCTC CCTTTTAGTT 
CACAAGCTTA TTTTTCAGTA CATTGAGGAA CAGGAATCCT GGAAGAAGAA ATGCACAGCA 
CAGCATCAGG TGCCCCAGAT GCTTCAAGAA CTCTCACTGG TAGTGAACCA TTGTAGGCTT 
CTTGGAGAGG AGATCGAATT TTTAAAGCGA TGGGGGCCAA ATTATAGCCT AATGCATATA 
AATGTGAATA ATACTGAATT GAGGCTTTTA TTCTCCAGTT GTGCAGCATT TGCAAAGTTT 
GAGATAACTT TGTCTCCCTC GGCCCATTAT CCCTTGGTCC CGTTACCGTT CACCATTCAC 
AATCACATTG GGAAGACTGG CCATGATGAA ATTGCTGCAA TTATCTCTAA AGTGCCCTTA 
GAGGAAAACT ACCTGAAGAA TGTAGTCAAG CAAATTTACC AAGATCTACT TAAGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006500439.1
CDS	443..6802
Translation	MDGVYSEANEENDNTQRPVRRQHSSILKPPRSPLQDLKCGNQTNQEPNPPRKRKSSRRVSFADTIKVFQTESHMKTERNSEISGMNTLLCAPIQTQMQQKEFSITDCNHERKHANDQTVIFSDENQMDLTASHTVMITKGLSDCTKNENSTKIDTTSFLENLKHHAANSRIKKDLACSTVSLSQNIFSEKINSDNFIKRLKTGKHISSSTELDKENAEIPVYSKDSNSASSTYQMHASLGVDENSSNRTRIFREQDDGMNLTQCHTACIKTWIPPSTEAKIGEFKGDKTIYGNECMELTTNYTIQVLSSENNLSERETQTQNGMNVTTVDGATAPAPEKKTALKDKLNAAFQGSFPNPENKIHIIKCHPIESETHTVTQISSQSASTLAVTSKSICSSPAIEGYKTIFHSSSNDAMELTKCLSAMEEEKKLLKADDKYSKICTNPDAGPLREKTIYLEEDSMDITKSHTVAIDNKIFKHDQENIKKEIIAIPIFEKEMVLRNLMPMSKDEKRDVNYISVPQVSKESLQRSQTNTLSVSLTDKKMEFLADEDMDLTKSHTTKLSQVIPTTFDLASKNVTKSYSHSKSPLNEWESLDKQVVLGQHSKLPLPQRKDRDDPDCSHHKIMYSEELQTMDLTKSHTIVIGFGPSEVQEHSKINLEHKNSQLTAESIQTAVNVPAANSRVVTTNDMDMLKDRSTHKPELLKEKQNIKIYGRKSIGRLKIDKTILFSEGNEGDMDITKSCTVKINHRSLLDKHDSHLVSLAGTSKTILHARGQVEMEINRSHTTPLECKIISPSDITPGDLDKTMMSIDDHEELDMTKSHTVFIDYQAEAKGVLPDRLDFQLSKKESLQKPKVTSLAEEIYISKNSESNHLPAKGSQLTILEEGSNSGLGEETNDAQKPGFLNELLSGKTQRRKSLSLKNKSITFPENDKSYREIPQSSAVEINNETRLEDRKGFSFVPLAGTSKPVLSAYGPEDMEISIGQTTASEYKTVPPEEITTIPMDKTVMFVDNFGDLDVTRSHTVFIDCQAKEKVLDEYTNLGIQKTKTLSGSEGDTHIQEITKNPAAQHKHHMTTVIPSSTVVSDQSSMKIKFHKADRDEEVKGKEVEANMLKQTKPESCLLNITDGKNVDFTSSYTADVCRSSDKYSSLPNISSSDNSGGNTMSLCDKNKEKAYNCQVPNEFTYAAILPSTYHMDSKKLSVFPPCPSKEVTQTESAIALLKDEDPVEEPLGEMATFNSKHVSLNLAKDQTEAFVDVSVASQPHLSAQQSPSTQKGQDVARRDEGILAKAGKKALPFLLENVAASTWENESKIPTNVEHFAVTYEKELSISIQTDKCNTNVQSPSNSALTTQVIQTHANAEGALDFLVPSTVSCFSSTKPSLSNLNRKTEEVLDFQTVNLLPPAEQLLEEGSQAHSMSIVQATEIYRLGSRNDRDEESKTFCNEAETTSVPLKTAVKDKTRRCSLGIFLPKLPSKRSCSITGVDDLEQILADAADLTQLETQPVCSKDPGIGSVAAKLNLSPSQFINEENLPVYPGEILSSDSVSLDIEESVLIDTSQRESLPSENKTENCRAQKRTRVEENDVTNEKKIRTHDSAQDQEIFDNHMEEDINKNVNSVLLKSLSRTPSSCSSSLDSIKSDGLSLDVSTQRNSQMESQFLGDTISEESLKEKLKDGQITIKEFFILLQVHILIQKPRQSTLPAKFTINTLPTTEDLMLRQYVYGPRIQIYKEDCELLRQKIDELKISALNQNKLLADVNRNLWEKVKDYSDEELKNYGIYLNKIKSRYTKMTKVFNHQGKVALYNKLVHSAENEKNKLQIKINEMDTILKKINNCLAEVETETKNLENEEKNDAMEEWDSEMRDAEKELEQLKTEEEELQRKFLEVETQKTQTLAQIEFIKEQTTKTEELLDQLSLSEWDVIEWSDDQAVFTFVYDSIELIITFGEPLVGLPFLDKACRKINALSFQSLLDEDKAPPSSLLVHKLIFQYIEEQESWKKKCTAQHQVPQMLQELSLVVNHCRLLGEEIEFLKRWGPNYSLMHINVNNTELRLLFSSCAAFAKFEITLSPSAHYPLVPLPFTIHNHIGKTGHDEIAAIISKVPLEENYLKNVVKQIYQDLLKD

Target ORF information:

RefSeq Version	XM_006500376.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinetochore scaffold 1 (Knl1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006500376.3

ORF Insert Sequence:

ATGGATGGGG TATATTCAGA AGCTAATGAA GAGAATGACA ATACACAGAG ACCTGTTAGA 
AGACAGCATT CCTCAATTTT GAAACCCCCA AGGAGTCCTC TTCAGGACCT CAAATGTGGA 
AATCAAACAA ACCAGGAACC TAATCCTCCA AGAAAAAGGA AAAGCTCTCG TCGAGTCAGC 
TTTGCAGATA CTATAAAAGT ATTCCAGACA GAGTCTCACA TGAAAACAGA GAGAAATTCA 
GAAATTTCAG GTATGAACAC TTTGCTTTGT GCTCCAATTC AGACTCAGAT GCAACAAAAG 
GAGTTCTCAA TTACAGACTG CAACCATGAA AGGAAACATG CAAATGATCA GACAGTCATC 
TTTTCAGATG AAAACCAGAT GGATCTGACA GCAAGTCACA CTGTAATGAT TACCAAAGGT 
CTTTCAGACT GTACCAAAAA TGAAAATTCT ACAAAAATAG ATACCACGTC CTTTCTGGAG 
AATTTAAAGC ATCATGCTGC AAATTCAAGA ATTAAAAAGG ATTTAGCATG TTCCACAGTT 
TCCTTAAGTC AAAACATTTT CTCAGAAAAG ATCAATTCTG ACAACTTCAT AAAAAGATTG 
AAGACAGGAA AACATATTTC GTCTTCTACA GAGCTTGATA AGGAAAATGC TGAGATACCT 
GTTTATTCCA AAGACTCAAA CAGTGCCTCT TCAACATATC AAATGCATGC ATCTCTTGGT 
GTAGATGAAA ATAGTAGTAA TAGGACTAGA ATCTTTAGAG AACAAGATGA TGGTATGAAT 
TTGACCCAAT GTCATACAGC CTGTATTAAG ACTTGGATTC CTCCATCCAC GGAGGCCAAA 
ATAGGAGAAT TCAAAGGTGA TAAAACAATT TATGGCAATG AGTGTATGGA GTTGACAACT 
AACTATACGA TACAGGTTTT GTCATCAGAA AATAATTTAT CTGAAAGAGA AACTCAAACT 
CAAAATGGTA TGAATGTTAC AACAGTTGAT GGAGCTACAG CTCCAGCTCC AGAGAAGAAA 
ACAGCCCTTA AGGATAAACT AAATGCTGCT TTTCAAGGCT CTTTCCCAAA CCCTGAAAAT 
AAAATTCATA TTATCAAATG TCATCCTATA GAGTCAGAAA CTCATACAGT CACACAGATT 
TCTAGTCAAA GTGCCAGCAC ATTGGCTGTA ACTTCAAAAT CTATATGTTC TAGTCCAGCT 
ATTGAGGGGT ATAAGACAAT TTTTCATTCT AGTAGCAATG ATGCAATGGA ACTGACTAAG 
TGTCTATCAG CTATGGAAGA GGAAAAAAAA TTGCTGAAGG CTGATGATAA GTATTCTAAA 
ATATGTACCA ATCCAGATGC TGGCCCACTC AGAGAGAAAA CTATTTATTT AGAAGAGGAT 
AGCATGGACA TTACCAAAAG TCACACCGTT GCAATAGATA ATAAAATATT TAAACATGAT 
CAAGAAAACA TTAAAAAAGA AATAATAGCC ATACCAATAT TTGAAAAGGA AATGGTGCTC 
CGAAATCTTA TGCCTATGTC AAAAGATGAG AAAAGGGATG TAAATTATAT TTCAGTTCCT 
CAAGTATCTA AGGAAAGCTT ACAACGGAGT CAGACAAACA CTTTATCTGT TTCATTGACT 
GACAAAAAGA TGGAATTCTT GGCAGATGAA GATATGGATC TGACTAAAAG TCACACAACT 
AAGTTAAGTC AAGTTATTCC TACAACTTTT GACTTAGCAT CAAAGAATGT CACCAAATCA 
TACTCTCACA GCAAAAGCCC TTTAAATGAG TGGGAAAGCC TGGATAAACA AGTAGTGCTA 
GGCCAGCATT CTAAACTTCC TTTACCACAG AGGAAAGATA GAGATGATCC TGACTGTTCT 
CATCATAAGA TAATGTATTC AGAGGAGTTA CAAACTATGG ATCTAACCAA GAGCCACACT 
ATTGTAATTG GCTTTGGTCC TTCTGAAGTA CAAGAACATA GTAAAATTAA TTTAGAACAC 
AAAAACAGCC AACTTACAGC AGAAAGCATA CAAACAGCTG TGAACGTTCC TGCAGCAAAC 
TCTAGAGTAG TAACAACTAA TGATATGGAC ATGTTAAAGG ACAGAAGTAC ACATAAGCCA 
GAATTGCTGA AGGAAAAGCA AAACATCAAA ATCTATGGAA GAAAAAGTAT TGGTAGACTG 
AAAATTGATA AAACTATATT ATTTTCAGAG GGTAATGAGG GTGATATGGA TATCACCAAG 
AGTTGCACAG TCAAAATAAA TCATAGATCT TTATTAGATA AGCATGATTC TCATTTAGTA 
TCTTTGGCAG GAACTTCTAA AACCATTTTG CATGCACGTG GTCAGGTTGA AATGGAAATC 
AATAGAAGTC ACACAACTCC TTTAGAATGT AAAATTATCT CGCCCAGTGA TATAACTCCT 
GGTGATCTGG ATAAGACTAT GATGTCTATA GATGATCATG AGGAACTAGA CATGACCAAG 
TCCCATACTG TTTTCATTGA CTACCAAGCA GAGGCAAAAG GTGTACTTCC AGACAGACTT 
GACTTTCAAT TATCCAAAAA GGAAAGCCTA CAGAAGCCGA AAGTGACATC TCTGGCTGAA 
GAGATTTACA TTTCAAAAAA TAGTGAAAGC AACCATCTAC CAGCAAAAGG CAGCCAGCTA 
ACAATATTAG AGGAAGGGTC TAATAGCGGG CTTGGAGAGG AAACTAATGA TGCACAAAAG 
CCTGGATTTC TGAATGAACT TCTGTCAGGC AAAACTCAGA GAAGAAAAAG CCTTAGCCTC 
AAAAACAAGA GTATTACATT TCCAGAGAAT GATAAAAGTT ATAGAGAGAT CCCTCAGAGT 
TCTGCAGTAG AAATAAATAA TGAAACTAGG CTGGAAGACA GAAAGGGCTT CAGTTTTGTG 
CCATTGGCAG GAACTTCCAA ACCAGTTTTG TCTGCATATG GGCCCGAGGA CATGGAGATT 
TCTATAGGTC AAACCACTGC TTCAGAATAT AAAACTGTCC CACCAGAAGA AATAACTACT 
ATACCTATGG ATAAAACTGT AATGTTTGTA GATAATTTCG GTGATCTGGA TGTCACCAGA 
TCCCATACGG TTTTCATCGA TTGTCAAGCC AAAGAAAAGG TACTTGATGA ATATACTAAC 
TTGGGAATAC AGAAAACAAA AACTTTGAGT GGTTCTGAAG GTGATACCCA TATTCAAGAA 
ATCACAAAAA ACCCAGCAGC TCAACACAAA CACCATATGA CAACTGTTAT TCCTTCTAGT 
ACAGTAGTTA GTGATCAAAG TTCAATGAAA ATCAAGTTTC ACAAAGCTGA TAGAGATGAG 
GAGGTCAAAG GAAAGGAAGT GGAAGCCAAT ATGCTGAAAC AAACTAAACC TGAAAGCTGT 
CTGTTAAATA TAACAGATGG AAAAAATGTG GATTTTACAA GCAGTTATAC AGCTGATGTT 
TGTAGATCCA GTGACAAGTA TTCCTCTTTA CCAAATATTT CTTCTTCTGA TAATTCGGGT 
GGTAATACTA TGTCCTTGTG TGATAAAAAT AAGGAGAAAG CTTATAATTG CCAAGTGCCA 
AATGAATTTA CTTATGCAGC TATCTTACCA AGTACATATC ACATGGATTC TAAAAAACTG 
TCTGTCTTTC CACCTTGTCC TTCAAAAGAA GTCACTCAAA CTGAAAGTGC CATAGCGCTG 
CTTAAAGATG AAGATCCGGT AGAAGAGCCT CTGGGGGAGA TGGCTACATT TAACTCAAAG 
CATGTATCTC TTAACCTGGC AAAGGATCAA ACAGAGGCCT TTGTTGATGT TAGTGTTGCT 
TCTCAGCCTC ATCTCTCAGC CCAACAAAGT CCATCAACTC AAAAAGGGCA GGATGTTGCC 
CGTAGAGATG AAGGAATATT AGCTAAAGCT GGGAAGAAGG CTTTACCTTT TCTTCTAGAA 
AATGTCGCTG CATCCACATG GGAAAATGAG TCCAAAATAC CCACTAATGT GGAACACTTT 
GCTGTAACCT ATGAAAAAGA ACTGAGTATA AGTATTCAAA CAGATAAATG TAATACAAAT 
GTACAGAGCC CTAGTAACTC GGCCCTGACA ACACAAGTCA TTCAGACGCA TGCAAATGCT 
GAAGGAGCAC TAGACTTTTT AGTTCCATCT ACTGTTTCAT GTTTTAGTAG TACCAAACCA 
AGTCTGAGTA ATTTGAATAG AAAAACTGAG GAGGTCTTAG ACTTTCAGAC TGTTAATTTA 
TTACCTCCTG CCGAACAGTT ACTTGAAGAA GGAAGTCAGG CACATAGTAT GAGTATAGTA 
CAAGCTACAG AAATATATAG ACTAGGTTCC AGGAATGATA GAGATGAAGA AAGTAAAACT 
TTCTGTAATG AGGCTGAAAC TACATCTGTA CCACTAAAAA CAGCTGTTAA AGACAAAACA 
AGAAGATGTT CATTGGGGAT ATTTTTACCC AAATTACCAA GCAAGAGAAG TTGTAGTATC 
ACTGGTGTTG ATGACCTGGA ACAGATTCTA GCAGATGCAG CTGACTTAAC TCAACTAGAA 
ACTCAGCCAG TCTGTAGTAA AGATCCAGGC ATTGGATCTG TTGCAGCCAA ACTGAATTTG 
AGTCCATCTC AGTTTATAAA TGAGGAAAAT CTCCCTGTAT ATCCTGGTGA GATACTTTCA 
TCAGATTCTG TTAGCCTGGA CATTGAGGAA AGTGTTCTGA TTGACACATC TCAGAGAGAG 
AGTTTACCGT CTGAAAACAA AACCGAAAAC TGCAGGGCCC AAAAAAGAAC CCGGGTTGAA 
GAAAATGATG TTACAAATGA GAAAAAAATC AGAACACATG ACAGTGCACA AGATCAAGAG 
ATTTTTGATA ATCATATGGA AGAGGATATC AATAAAAATG TCAACAGTGT ATTGCTGAAA 
AGCCTGAGCA GGACACCGTC TAGTTGCAGC AGCTCTCTGG ATTCAATCAA GTCTGATGGA 
CTCTCTCTGG ATGTCAGCAC ACAGCGTAAT AGCCAAATGG AATCACAGTT TCTTGGTGAC 
ACTATCTCTG AAGAGAGTTT GAAGGAGAAA CTCAAGGATG GACAAATTAC AATAAAGGAG 
TTCTTCATAC TTCTTCAGGT CCACATCTTA ATACAGAAAC CTAGGCAGAG CACTCTCCCA 
GCCAAATTTA CTATAAACAC ATTACCTACC ACAGAAGATC TGATGTTGAG ACAATATGTT 
TATGGACCCA GGATACAGAT TTATAAAGAA GACTGTGAGC TTCTTCGACA AAAGATTGAC 
GAATTAAAGA TATCTGCATT GAACCAAAAT AAACTATTGG CGGATGTAAA TAGGAACTTG 
TGGGAAAAAG TGAAAGACTA CTCTGATGAA GAGCTGAAGA ACTATGGGAT TTACCTTAAC 
AAAATCAAAT CACGTTATAC CAAGATGACA AAAGTCTTCA ATCACCAAGG AAAAGTGGCT 
CTGTATAACA AATTGGTGCA TTCAGCTGAG AATGAGAAGA ACAAACTTCA GATAAAAATA 
AATGAGATGG ACACTATCCT CAAGAAGATC AATAACTGTC TCGCTGAAGT GGAAACAGAA 
ACTAAGAATT TGGAGAATGA AGAGAAAAAT GATGCCATGG AAGAATGGGA TTCTGAAATG 
AGAGATGCAG AGAAAGAACT GGAACAGCTG AAAACTGAAG AAGAGGAGCT TCAGAGAAAG 
TTCTTAGAAG TAGAGACACA GAAAACGCAG ACCCTTGCTC AGATAGAATT TATAAAAGAA 
CAAACAACTA AAACTGAAGA ATTACTGGAT CAATTGAGTT TGTCTGAGTG GGATGTCATT 
GAGTGGAGTG ATGATCAAGC TGTATTTACC TTTGTCTATG ATTCAATAGA ACTCATCATC 
ACCTTTGGAG AGCCACTAGT TGGTCTCCCT TTTTTGGACA AGGCTTGCAG GAAGATTAAT 
GCACTCAGTT TTCAGTCTCT GTTAGATGAA GATAAAGCTC CTCCCTCCTC CCTTTTAGTT 
CACAAGCTTA TTTTTCAGTA CATTGAGGAA CAGGAATCCT GGAAGAAGAA ATGCACAGCA 
CAGCATCAGG TGCCCCAGAT GCTTCAAGAA CTCTCACTGG TAGTGAACCA TTGTAGGCTT 
CTTGGAGAGG AGATCGAATT TTTAAAGCGA TGGGGGCCAA ATTATAGCCT AATGCATATA 
AATGTGAATA ATACTGAATT GAGGCTTTTA TTCTCCAGTT GTGCAGCATT TGCAAAGTTT 
GAGATAACTT TGTCTCCCTC GGCCCATTAT CCCTTGGTCC CGTTACCGTT CACCATTCAC 
AATCACATTG GGAAGACTGG CCATGATGAA ATTGCTGCAA TTATCTCTAA AGTGCCCTTA 
GAGGAAAACT ACCTGAAGAA TGTAGTCAAG CAAATTTACC AAGATCTACT TAAGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Knl1 ( XM_030252192.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030108052.1 Mus musculus kinetochore scaffold 1 (Knl1), transcript variant X3, mRNA.

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CloneID	OMu88036
Clone ID Related Accession (Same CDS sequence)	XM_030252192.1
Accession Version	XM_030252192.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6141bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kinetochore scaffold 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAACAG AGAGAAATTC AGAAATTTCA GGTATGAACA CTTTGCTTTG TGCTCCAATT 
CAGACTCAGA TGCAACAAAA GGAGTTCTCA ATTACAGACT GCAACCATGA AAGGAAACAT 
GCAAATGATC AGACAGTCAT CTTTTCAGAT GAAAACCAGA TGGATCTGAC AGCAAGTCAC 
ACTGTAATGA TTACCAAAGG TCTTTCAGAC TGTACCAAAA ATGAAAATTC TACAAAAATA 
GATACCACGT CCTTTCTGGA GAATTTAAAG CATCATGCTG CAAATTCAAG AATTAAAAAG 
GATTTAGCAT GTTCCACAGT TTCCTTAAGT CAAAACATTT TCTCAGAAAA GATCAATTCT 
GACAACTTCA TAAAAAGATT GAAGACAGGA AAACATATTT CGTCTTCTAC AGAGCTTGAT 
AAGGAAAATG CTGAGATACC TGTTTATTCC AAAGACTCAA ACAGTGCCTC TTCAACATAT 
CAAATGCATG CATCTCTTGG TGTAGATGAA AATAGTAGTA ATAGGACTAG AATCTTTAGA 
GAACAAGATG ATGGTATGAA TTTGACCCAA TGTCATACAG CCTGTATTAA GACTTGGATT 
CCTCCATCCA CGGAGGCCAA AATAGGAGAA TTCAAAGGTG ATAAAACAAT TTATGGCAAT 
GAGTGTATGG AGTTGACAAC TAACTATACG ATACAGGTTT TGTCATCAGA AAATAATTTA 
TCTGAAAGAG AAACTCAAAC TCAAAATGGT ATGAATGTTA CAACAGTTGA TGGAGCTACA 
GCTCCAGCTC CAGAGAAGAA AACAGCCCTT AAGGATAAAC TAAATGCTGC TTTTCAAGGC 
TCTTTCCCAA ACCCTGAAAA TAAAATTCAT ATTATCAAAT GTCATCCTAT AGAGTCAGAA 
ACTCATACAG TCACACAGAT TTCTAGTCAA AGTGCCAGCA CATTGGCTGT AACTTCAAAA 
TCTATATGTT CTAGTCCAGC TATTGAGGGG TATAAGACAA TTTTTCATTC TAGTAGCAAT 
GATGCAATGG AACTGACTAA GTGTCTATCA GCTATGGAAG AGGAAAAAAA ATTGCTGAAG 
GCTGATGATA AGTATTCTAA AATATGTACC AATCCAGATG CTGGCCCACT CAGAGAGAAA 
ACTATTTATT TAGAAGAGGA TAGCATGGAC ATTACCAAAA GTCACACCGT TGCAATAGAT 
AATAAAATAT TTAAACATGA TCAAGAAAAC ATTAAAAAAG AAATAATAGC CATACCAATA 
TTTGAAAAGG AAATGGTGCT CCGAAATCTT ATGCCTATGT CAAAAGATGA GAAAAGGGAT 
GTAAATTATA TTTCAGTTCC TCAAGTATCT AAGGAAAGCT TACAACGGAG TCAGACAAAC 
ACTTTATCTG TTTCATTGAC TGACAAAAAG ATGGAATTCT TGGCAGATGA AGATATGGAT 
CTGACTAAAA GTCACACAAC TAAGTTAAGT CAAGTTATTC CTACAACTTT TGACTTAGCA 
TCAAAGAATG TCACCAAATC ATACTCTCAC AGCAAAAGCC CTTTAAATGA GTGGGAAAGC 
CTGGATAAAC AAGTAGTGCT AGGCCAGCAT TCTAAACTTC CTTTACCACA GAGGAAAGAT 
AGAGATGATC CTGACTGTTC TCATCATAAG ATAATGTATT CAGAGGAGTT ACAAACTATG 
GATCTAACCA AGAGCCACAC TATTGTAATT GGCTTTGGTC CTTCTGAAGT ACAAGAACAT 
AGTAAAATTA ATTTAGAACA CAAAAACAGC CAACTTACAG CAGAAAGCAT ACAAACAGCT 
GTGAACGTTC CTGCAGCAAA CTCTAGAGTA GTAACAACTA ATGATATGGA CATGTTAAAG 
GACAGAAGTA CACATAAGCC AGAATTGCTG AAGGAAAAGC AAAACATCAA AATCTATGGA 
AGAAAAAGTA TTGGTAGACT GAAAATTGAT AAAACTATAT TATTTTCAGA GGGTAATGAG 
GGTGATATGG ATATCACCAA GAGTTGCACA GTCAAAATAA ATCATAGATC TTTATTAGAT 
AAGCATGATT CTCATTTAGT ATCTTTGGCA GGAACTTCTA AAACCATTTT GCATGCACGT 
GGTCAGGTTG AAATGGAAAT CAATAGAAGT CACACAACTC CTTTAGAATG TAAAATTATC 
TCGCCCAGTG ATATAACTCC TGGTGATCTG GATAAGACTA TGATGTCTAT AGATGATCAT 
GAGGAACTAG ACATGACCAA GTCCCATACT GTTTTCATTG ACTACCAAGC AGAGGCAAAA 
GGTGTACTTC CAGACAGACT TGACTTTCAA TTATCCAAAA AGGAAAGCCT ACAGAAGCCG 
AAAGTGACAT CTCTGGCTGA AGAGATTTAC ATTTCAAAAA ATAGTGAAAG CAACCATCTA 
CCAGCAAAAG GCAGCCAGCT AACAATATTA GAGGAAGGGT CTAATAGCGG GCTTGGAGAG 
GAAACTAATG ATGCACAAAA GCCTGGATTT CTGAATGAAC TTCTGTCAGG CAAAACTCAG 
AGAAGAAAAA GCCTTAGCCT CAAAAACAAG AGTATTACAT TTCCAGAGAA TGATAAAAGT 
TATAGAGAGA TCCCTCAGAG TTCTGCAGTA GAAATAAATA ATGAAACTAG GCTGGAAGAC 
AGAAAGGGCT TCAGTTTTGT GCCATTGGCA GGAACTTCCA AACCAGTTTT GTCTGCATAT 
GGGCCCGAGG ACATGGAGAT TTCTATAGGT CAAACCACTG CTTCAGAATA TAAAACTGTC 
CCACCAGAAG AAATAACTAC TATACCTATG GATAAAACTG TAATGTTTGT AGATAATTTC 
GGTGATCTGG ATGTCACCAG ATCCCATACG GTTTTCATCG ATTGTCAAGC CAAAGAAAAG 
GTACTTGATG AATATACTAA CTTGGGAATA CAGAAAACAA AAACTTTGAG TGGTTCTGAA 
GGTGATACCC ATATTCAAGA AATCACAAAA AACCCAGCAG CTCAACACAA ACACCATATG 
ACAACTGTTA TTCCTTCTAG TACAGTAGTT AGTGATCAAA GTTCAATGAA AATCAAGTTT 
CACAAAGCTG ATAGAGATGA GGAGGTCAAA GGAAAGGAAG TGGAAGCCAA TATGCTGAAA 
CAAACTAAAC CTGAAAGCTG TCTGTTAAAT ATAACAGATG GAAAAAATGT GGATTTTACA 
AGCAGTTATA CAGCTGATGT TTGTAGATCC AGTGACAAGT ATTCCTCTTT ACCAAATATT 
TCTTCTTCTG ATAATTCGGG TGGTAATACT ATGTCCTTGT GTGATAAAAA TAAGGAGAAA 
GCTTATAATT GCCAAGTGCC AAATGAATTT ACTTATGCAG CTATCTTACC AAGTACATAT 
CACATGGATT CTAAAAAACT GTCTGTCTTT CCACCTTGTC CTTCAAAAGA AGTCACTCAA 
ACTGAAAGTG CCATAGCGCT GCTTAAAGAT GAAGATCCGG TAGAAGAGCC TCTGGGGGAG 
ATGGCTACAT TTAACTCAAA GCATGTATCT CTTAACCTGG CAAAGGATCA AACAGAGGCC 
TTTGTTGATG TTAGTGTTGC TTCTCAGCCT CATCTCTCAG CCCAACAAAG TCCATCAACT 
CAAAAAGGGC AGGATGTTGC CCGTAGAGAT GAAGGAATAT TAGCTAAAGC TGGGAAGAAG 
GCTTTACCTT TTCTTCTAGA AAATGTCGCT GCATCCACAT GGGAAAATGA GTCCAAAATA 
CCCACTAATG TGGAACACTT TGCTGTAACC TATGAAAAAG AACTGAGTAT AAGTATTCAA 
ACAGATAAAT GTAATACAAA TGTACAGAGC CCTAGTAACT CGGCCCTGAC AACACAAGTC 
ATTCAGACGC ATGCAAATGC TGAAGGAGCA CTAGACTTTT TAGTTCCATC TACTGTTTCA 
TGTTTTAGTA GTACCAAACC AAGTCTGAGT AATTTGAATA GAAAAACTGA GGAGGTCTTA 
GACTTTCAGA CTGTTAATTT ATTACCTCCT GCCGAACAGT TACTTGAAGA AGGAAGTCAG 
GCACATAGTA TGAGTATAGT ACAAGCTACA GAAATATATA GACTAGGTTC CAGGAATGAT 
AGAGATGAAG AAAGTAAAAC TTTCTGTAAT GAGGCTGAAA CTACATCTGT ACCACTAAAA 
ACAGCTGTTA AAGACAAAAC AAGAAGATGT TCATTGGGGA TATTTTTACC CAAATTACCA 
AGCAAGAGAA GTTGTAGTAT CACTGGTGTT GATGACCTGG AACAGATTCT AGCAGATGCA 
GCTGACTTAA CTCAACTAGA AACTCAGCCA GTCTGTAGTA AAGATCCAGG CATTGGATCT 
GTTGCAGCCA AACTGAATTT GAGTCCATCT CAGTTTATAA ATGAGGAAAA TCTCCCTGTA 
TATCCTGGTG AGATACTTTC ATCAGATTCT GTTAGCCTGG ACATTGAGGA AAGTGTTCTG 
ATTGACACAT CTCAGAGAGA GAGTTTACCG TCTGAAAACA AAACCGAAAA CTGCAGGGCC 
CAAAAAAGAA CCCGGGTTGA AGAAAATGAT GTTACAAATG AGAAAAAAAT CAGAACACAT 
GACAGTGCAC AAGATCAAGA GATTTTTGAT AATCATATGG AAGAGGATAT CAATAAAAAT 
GTCAACAGTG TATTGCTGAA AAGCCTGAGC AGGACACCGT CTAGTTGCAG CAGCTCTCTG 
GATTCAATCA AGTCTGATGG ACTCTCTCTG GATGTCAGCA CACAGCGTAA TAGCCAAATG 
GAATCACAGT TTCTTGGTGA CACTATCTCT GAAGAGAGTT TGAAGGAGAA ACTCAAGGAT 
GGACAAATTA CAATAAAGGA GTTCTTCATA CTTCTTCAGG TCCACATCTT AATACAGAAA 
CCTAGGCAGA GCACTCTCCC AGCCAAATTT ACTATAAACA CATTACCTAC CACAGAAGAT 
CTGATGTTGA GACAATATGT TTATGGACCC AGGATACAGA TTTATAAAGA AGACTGTGAG 
CTTCTTCGAC AAAAGATTGA CGAATTAAAG ATATCTGCAT TGAACCAAAA TAAACTATTG 
GCGGATGTAA ATAGGAACTT GTGGGAAAAA GTGAAAGACT ACTCTGATGA AGAGCTGAAG 
AACTATGGGA TTTACCTTAA CAAAATCAAA TCACGTTATA CCAAGATGAC AAAAGTCTTC 
AATCACCAAG GAAAAGTGGC TCTGTATAAC AAATTGGTGC ATTCAGCTGA GAATGAGAAG 
AACAAACTTC AGATAAAAAT AAATGAGATG GACACTATCC TCAAGAAGAT CAATAACTGT 
CTCGCTGAAG TGGAAACAGA AACTAAGAAT TTGGAGAATG AAGAGAAAAA TGATGCCATG 
GAAGAATGGG ATTCTGAAAT GAGAGATGCA GAGAAAGAAC TGGAACAGCT GAAAACTGAA 
GAAGAGGAGC TTCAGAGAAA GTTCTTAGAA GTAGAGACAC AGAAAACGCA GACCCTTGCT 
CAGATAGAAT TTATAAAAGA ACAAACAACT AAAACTGAAG AATTACTGGA TCAATTGAGT 
TTGTCTGAGT GGGATGTCAT TGAGTGGAGT GATGATCAAG CTGTATTTAC CTTTGTCTAT 
GATTCAATAG AACTCATCAT CACCTTTGGA GAGCCACTAG TTGGTCTCCC TTTTTTGGAC 
AAGGCTTGCA GGAAGATTAA TGCACTCAGT TTTCAGTCTC TGTTAGATGA AGATAAAGCT 
CCTCCCTCCT CCCTTTTAGT TCACAAGCTT ATTTTTCAGT ACATTGAGGA ACAGGAATCC 
TGGAAGAAGA AATGCACAGC ACAGCATCAG GTGCCCCAGA TGCTTCAAGA ACTCTCACTG 
GTAGTGAACC ATTGTAGGCT TCTTGGAGAG GAGATCGAAT TTTTAAAGCG ATGGGGGCCA 
AATTATAGCC TAATGCATAT AAATGTGAAT AATACTGAAT TGAGGCTTTT ATTCTCCAGT 
TGTGCAGCAT TTGCAAAGTT TGAGATAACT TTGTCTCCCT CGGCCCATTA TCCCTTGGTC 
CCGTTACCGT TCACCATTCA CAATCACATT GGGAAGACTG GCCATGATGA AATTGCTGCA 
ATTATCTCTA AAGTGCCCTT AGAGGAAAAC TACCTGAAGA ATGTAGTCAA GCAAATTTAC 
CAAGATCTAC TTAAGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030108052.1
CDS	599..6739
Translation	MKTERNSEISGMNTLLCAPIQTQMQQKEFSITDCNHERKHANDQTVIFSDENQMDLTASHTVMITKGLSDCTKNENSTKIDTTSFLENLKHHAANSRIKKDLACSTVSLSQNIFSEKINSDNFIKRLKTGKHISSSTELDKENAEIPVYSKDSNSASSTYQMHASLGVDENSSNRTRIFREQDDGMNLTQCHTACIKTWIPPSTEAKIGEFKGDKTIYGNECMELTTNYTIQVLSSENNLSERETQTQNGMNVTTVDGATAPAPEKKTALKDKLNAAFQGSFPNPENKIHIIKCHPIESETHTVTQISSQSASTLAVTSKSICSSPAIEGYKTIFHSSSNDAMELTKCLSAMEEEKKLLKADDKYSKICTNPDAGPLREKTIYLEEDSMDITKSHTVAIDNKIFKHDQENIKKEIIAIPIFEKEMVLRNLMPMSKDEKRDVNYISVPQVSKESLQRSQTNTLSVSLTDKKMEFLADEDMDLTKSHTTKLSQVIPTTFDLASKNVTKSYSHSKSPLNEWESLDKQVVLGQHSKLPLPQRKDRDDPDCSHHKIMYSEELQTMDLTKSHTIVIGFGPSEVQEHSKINLEHKNSQLTAESIQTAVNVPAANSRVVTTNDMDMLKDRSTHKPELLKEKQNIKIYGRKSIGRLKIDKTILFSEGNEGDMDITKSCTVKINHRSLLDKHDSHLVSLAGTSKTILHARGQVEMEINRSHTTPLECKIISPSDITPGDLDKTMMSIDDHEELDMTKSHTVFIDYQAEAKGVLPDRLDFQLSKKESLQKPKVTSLAEEIYISKNSESNHLPAKGSQLTILEEGSNSGLGEETNDAQKPGFLNELLSGKTQRRKSLSLKNKSITFPENDKSYREIPQSSAVEINNETRLEDRKGFSFVPLAGTSKPVLSAYGPEDMEISIGQTTASEYKTVPPEEITTIPMDKTVMFVDNFGDLDVTRSHTVFIDCQAKEKVLDEYTNLGIQKTKTLSGSEGDTHIQEITKNPAAQHKHHMTTVIPSSTVVSDQSSMKIKFHKADRDEEVKGKEVEANMLKQTKPESCLLNITDGKNVDFTSSYTADVCRSSDKYSSLPNISSSDNSGGNTMSLCDKNKEKAYNCQVPNEFTYAAILPSTYHMDSKKLSVFPPCPSKEVTQTESAIALLKDEDPVEEPLGEMATFNSKHVSLNLAKDQTEAFVDVSVASQPHLSAQQSPSTQKGQDVARRDEGILAKAGKKALPFLLENVAASTWENESKIPTNVEHFAVTYEKELSISIQTDKCNTNVQSPSNSALTTQVIQTHANAEGALDFLVPSTVSCFSSTKPSLSNLNRKTEEVLDFQTVNLLPPAEQLLEEGSQAHSMSIVQATEIYRLGSRNDRDEESKTFCNEAETTSVPLKTAVKDKTRRCSLGIFLPKLPSKRSCSITGVDDLEQILADAADLTQLETQPVCSKDPGIGSVAAKLNLSPSQFINEENLPVYPGEILSSDSVSLDIEESVLIDTSQRESLPSENKTENCRAQKRTRVEENDVTNEKKIRTHDSAQDQEIFDNHMEEDINKNVNSVLLKSLSRTPSSCSSSLDSIKSDGLSLDVSTQRNSQMESQFLGDTISEESLKEKLKDGQITIKEFFILLQVHILIQKPRQSTLPAKFTINTLPTTEDLMLRQYVYGPRIQIYKEDCELLRQKIDELKISALNQNKLLADVNRNLWEKVKDYSDEELKNYGIYLNKIKSRYTKMTKVFNHQGKVALYNKLVHSAENEKNKLQIKINEMDTILKKINNCLAEVETETKNLENEEKNDAMEEWDSEMRDAEKELEQLKTEEEELQRKFLEVETQKTQTLAQIEFIKEQTTKTEELLDQLSLSEWDVIEWSDDQAVFTFVYDSIELIITFGEPLVGLPFLDKACRKINALSFQSLLDEDKAPPSSLLVHKLIFQYIEEQESWKKKCTAQHQVPQMLQELSLVVNHCRLLGEEIEFLKRWGPNYSLMHINVNNTELRLLFSSCAAFAKFEITLSPSAHYPLVPLPFTIHNHIGKTGHDEIAAIISKVPLEENYLKNVVKQIYQDLLKD

Target ORF information:

RefSeq Version	XM_030252192.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinetochore scaffold 1 (Knl1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030252192.1

ORF Insert Sequence:

ATGAAAACAG AGAGAAATTC AGAAATTTCA GGTATGAACA CTTTGCTTTG TGCTCCAATT 
CAGACTCAGA TGCAACAAAA GGAGTTCTCA ATTACAGACT GCAACCATGA AAGGAAACAT 
GCAAATGATC AGACAGTCAT CTTTTCAGAT GAAAACCAGA TGGATCTGAC AGCAAGTCAC 
ACTGTAATGA TTACCAAAGG TCTTTCAGAC TGTACCAAAA ATGAAAATTC TACAAAAATA 
GATACCACGT CCTTTCTGGA GAATTTAAAG CATCATGCTG CAAATTCAAG AATTAAAAAG 
GATTTAGCAT GTTCCACAGT TTCCTTAAGT CAAAACATTT TCTCAGAAAA GATCAATTCT 
GACAACTTCA TAAAAAGATT GAAGACAGGA AAACATATTT CGTCTTCTAC AGAGCTTGAT 
AAGGAAAATG CTGAGATACC TGTTTATTCC AAAGACTCAA ACAGTGCCTC TTCAACATAT 
CAAATGCATG CATCTCTTGG TGTAGATGAA AATAGTAGTA ATAGGACTAG AATCTTTAGA 
GAACAAGATG ATGGTATGAA TTTGACCCAA TGTCATACAG CCTGTATTAA GACTTGGATT 
CCTCCATCCA CGGAGGCCAA AATAGGAGAA TTCAAAGGTG ATAAAACAAT TTATGGCAAT 
GAGTGTATGG AGTTGACAAC TAACTATACG ATACAGGTTT TGTCATCAGA AAATAATTTA 
TCTGAAAGAG AAACTCAAAC TCAAAATGGT ATGAATGTTA CAACAGTTGA TGGAGCTACA 
GCTCCAGCTC CAGAGAAGAA AACAGCCCTT AAGGATAAAC TAAATGCTGC TTTTCAAGGC 
TCTTTCCCAA ACCCTGAAAA TAAAATTCAT ATTATCAAAT GTCATCCTAT AGAGTCAGAA 
ACTCATACAG TCACACAGAT TTCTAGTCAA AGTGCCAGCA CATTGGCTGT AACTTCAAAA 
TCTATATGTT CTAGTCCAGC TATTGAGGGG TATAAGACAA TTTTTCATTC TAGTAGCAAT 
GATGCAATGG AACTGACTAA GTGTCTATCA GCTATGGAAG AGGAAAAAAA ATTGCTGAAG 
GCTGATGATA AGTATTCTAA AATATGTACC AATCCAGATG CTGGCCCACT CAGAGAGAAA 
ACTATTTATT TAGAAGAGGA TAGCATGGAC ATTACCAAAA GTCACACCGT TGCAATAGAT 
AATAAAATAT TTAAACATGA TCAAGAAAAC ATTAAAAAAG AAATAATAGC CATACCAATA 
TTTGAAAAGG AAATGGTGCT CCGAAATCTT ATGCCTATGT CAAAAGATGA GAAAAGGGAT 
GTAAATTATA TTTCAGTTCC TCAAGTATCT AAGGAAAGCT TACAACGGAG TCAGACAAAC 
ACTTTATCTG TTTCATTGAC TGACAAAAAG ATGGAATTCT TGGCAGATGA AGATATGGAT 
CTGACTAAAA GTCACACAAC TAAGTTAAGT CAAGTTATTC CTACAACTTT TGACTTAGCA 
TCAAAGAATG TCACCAAATC ATACTCTCAC AGCAAAAGCC CTTTAAATGA GTGGGAAAGC 
CTGGATAAAC AAGTAGTGCT AGGCCAGCAT TCTAAACTTC CTTTACCACA GAGGAAAGAT 
AGAGATGATC CTGACTGTTC TCATCATAAG ATAATGTATT CAGAGGAGTT ACAAACTATG 
GATCTAACCA AGAGCCACAC TATTGTAATT GGCTTTGGTC CTTCTGAAGT ACAAGAACAT 
AGTAAAATTA ATTTAGAACA CAAAAACAGC CAACTTACAG CAGAAAGCAT ACAAACAGCT 
GTGAACGTTC CTGCAGCAAA CTCTAGAGTA GTAACAACTA ATGATATGGA CATGTTAAAG 
GACAGAAGTA CACATAAGCC AGAATTGCTG AAGGAAAAGC AAAACATCAA AATCTATGGA 
AGAAAAAGTA TTGGTAGACT GAAAATTGAT AAAACTATAT TATTTTCAGA GGGTAATGAG 
GGTGATATGG ATATCACCAA GAGTTGCACA GTCAAAATAA ATCATAGATC TTTATTAGAT 
AAGCATGATT CTCATTTAGT ATCTTTGGCA GGAACTTCTA AAACCATTTT GCATGCACGT 
GGTCAGGTTG AAATGGAAAT CAATAGAAGT CACACAACTC CTTTAGAATG TAAAATTATC 
TCGCCCAGTG ATATAACTCC TGGTGATCTG GATAAGACTA TGATGTCTAT AGATGATCAT 
GAGGAACTAG ACATGACCAA GTCCCATACT GTTTTCATTG ACTACCAAGC AGAGGCAAAA 
GGTGTACTTC CAGACAGACT TGACTTTCAA TTATCCAAAA AGGAAAGCCT ACAGAAGCCG 
AAAGTGACAT CTCTGGCTGA AGAGATTTAC ATTTCAAAAA ATAGTGAAAG CAACCATCTA 
CCAGCAAAAG GCAGCCAGCT AACAATATTA GAGGAAGGGT CTAATAGCGG GCTTGGAGAG 
GAAACTAATG ATGCACAAAA GCCTGGATTT CTGAATGAAC TTCTGTCAGG CAAAACTCAG 
AGAAGAAAAA GCCTTAGCCT CAAAAACAAG AGTATTACAT TTCCAGAGAA TGATAAAAGT 
TATAGAGAGA TCCCTCAGAG TTCTGCAGTA GAAATAAATA ATGAAACTAG GCTGGAAGAC 
AGAAAGGGCT TCAGTTTTGT GCCATTGGCA GGAACTTCCA AACCAGTTTT GTCTGCATAT 
GGGCCCGAGG ACATGGAGAT TTCTATAGGT CAAACCACTG CTTCAGAATA TAAAACTGTC 
CCACCAGAAG AAATAACTAC TATACCTATG GATAAAACTG TAATGTTTGT AGATAATTTC 
GGTGATCTGG ATGTCACCAG ATCCCATACG GTTTTCATCG ATTGTCAAGC CAAAGAAAAG 
GTACTTGATG AATATACTAA CTTGGGAATA CAGAAAACAA AAACTTTGAG TGGTTCTGAA 
GGTGATACCC ATATTCAAGA AATCACAAAA AACCCAGCAG CTCAACACAA ACACCATATG 
ACAACTGTTA TTCCTTCTAG TACAGTAGTT AGTGATCAAA GTTCAATGAA AATCAAGTTT 
CACAAAGCTG ATAGAGATGA GGAGGTCAAA GGAAAGGAAG TGGAAGCCAA TATGCTGAAA 
CAAACTAAAC CTGAAAGCTG TCTGTTAAAT ATAACAGATG GAAAAAATGT GGATTTTACA 
AGCAGTTATA CAGCTGATGT TTGTAGATCC AGTGACAAGT ATTCCTCTTT ACCAAATATT 
TCTTCTTCTG ATAATTCGGG TGGTAATACT ATGTCCTTGT GTGATAAAAA TAAGGAGAAA 
GCTTATAATT GCCAAGTGCC AAATGAATTT ACTTATGCAG CTATCTTACC AAGTACATAT 
CACATGGATT CTAAAAAACT GTCTGTCTTT CCACCTTGTC CTTCAAAAGA AGTCACTCAA 
ACTGAAAGTG CCATAGCGCT GCTTAAAGAT GAAGATCCGG TAGAAGAGCC TCTGGGGGAG 
ATGGCTACAT TTAACTCAAA GCATGTATCT CTTAACCTGG CAAAGGATCA AACAGAGGCC 
TTTGTTGATG TTAGTGTTGC TTCTCAGCCT CATCTCTCAG CCCAACAAAG TCCATCAACT 
CAAAAAGGGC AGGATGTTGC CCGTAGAGAT GAAGGAATAT TAGCTAAAGC TGGGAAGAAG 
GCTTTACCTT TTCTTCTAGA AAATGTCGCT GCATCCACAT GGGAAAATGA GTCCAAAATA 
CCCACTAATG TGGAACACTT TGCTGTAACC TATGAAAAAG AACTGAGTAT AAGTATTCAA 
ACAGATAAAT GTAATACAAA TGTACAGAGC CCTAGTAACT CGGCCCTGAC AACACAAGTC 
ATTCAGACGC ATGCAAATGC TGAAGGAGCA CTAGACTTTT TAGTTCCATC TACTGTTTCA 
TGTTTTAGTA GTACCAAACC AAGTCTGAGT AATTTGAATA GAAAAACTGA GGAGGTCTTA 
GACTTTCAGA CTGTTAATTT ATTACCTCCT GCCGAACAGT TACTTGAAGA AGGAAGTCAG 
GCACATAGTA TGAGTATAGT ACAAGCTACA GAAATATATA GACTAGGTTC CAGGAATGAT 
AGAGATGAAG AAAGTAAAAC TTTCTGTAAT GAGGCTGAAA CTACATCTGT ACCACTAAAA 
ACAGCTGTTA AAGACAAAAC AAGAAGATGT TCATTGGGGA TATTTTTACC CAAATTACCA 
AGCAAGAGAA GTTGTAGTAT CACTGGTGTT GATGACCTGG AACAGATTCT AGCAGATGCA 
GCTGACTTAA CTCAACTAGA AACTCAGCCA GTCTGTAGTA AAGATCCAGG CATTGGATCT 
GTTGCAGCCA AACTGAATTT GAGTCCATCT CAGTTTATAA ATGAGGAAAA TCTCCCTGTA 
TATCCTGGTG AGATACTTTC ATCAGATTCT GTTAGCCTGG ACATTGAGGA AAGTGTTCTG 
ATTGACACAT CTCAGAGAGA GAGTTTACCG TCTGAAAACA AAACCGAAAA CTGCAGGGCC 
CAAAAAAGAA CCCGGGTTGA AGAAAATGAT GTTACAAATG AGAAAAAAAT CAGAACACAT 
GACAGTGCAC AAGATCAAGA GATTTTTGAT AATCATATGG AAGAGGATAT CAATAAAAAT 
GTCAACAGTG TATTGCTGAA AAGCCTGAGC AGGACACCGT CTAGTTGCAG CAGCTCTCTG 
GATTCAATCA AGTCTGATGG ACTCTCTCTG GATGTCAGCA CACAGCGTAA TAGCCAAATG 
GAATCACAGT TTCTTGGTGA CACTATCTCT GAAGAGAGTT TGAAGGAGAA ACTCAAGGAT 
GGACAAATTA CAATAAAGGA GTTCTTCATA CTTCTTCAGG TCCACATCTT AATACAGAAA 
CCTAGGCAGA GCACTCTCCC AGCCAAATTT ACTATAAACA CATTACCTAC CACAGAAGAT 
CTGATGTTGA GACAATATGT TTATGGACCC AGGATACAGA TTTATAAAGA AGACTGTGAG 
CTTCTTCGAC AAAAGATTGA CGAATTAAAG ATATCTGCAT TGAACCAAAA TAAACTATTG 
GCGGATGTAA ATAGGAACTT GTGGGAAAAA GTGAAAGACT ACTCTGATGA AGAGCTGAAG 
AACTATGGGA TTTACCTTAA CAAAATCAAA TCACGTTATA CCAAGATGAC AAAAGTCTTC 
AATCACCAAG GAAAAGTGGC TCTGTATAAC AAATTGGTGC ATTCAGCTGA GAATGAGAAG 
AACAAACTTC AGATAAAAAT AAATGAGATG GACACTATCC TCAAGAAGAT CAATAACTGT 
CTCGCTGAAG TGGAAACAGA AACTAAGAAT TTGGAGAATG AAGAGAAAAA TGATGCCATG 
GAAGAATGGG ATTCTGAAAT GAGAGATGCA GAGAAAGAAC TGGAACAGCT GAAAACTGAA 
GAAGAGGAGC TTCAGAGAAA GTTCTTAGAA GTAGAGACAC AGAAAACGCA GACCCTTGCT 
CAGATAGAAT TTATAAAAGA ACAAACAACT AAAACTGAAG AATTACTGGA TCAATTGAGT 
TTGTCTGAGT GGGATGTCAT TGAGTGGAGT GATGATCAAG CTGTATTTAC CTTTGTCTAT 
GATTCAATAG AACTCATCAT CACCTTTGGA GAGCCACTAG TTGGTCTCCC TTTTTTGGAC 
AAGGCTTGCA GGAAGATTAA TGCACTCAGT TTTCAGTCTC TGTTAGATGA AGATAAAGCT 
CCTCCCTCCT CCCTTTTAGT TCACAAGCTT ATTTTTCAGT ACATTGAGGA ACAGGAATCC 
TGGAAGAAGA AATGCACAGC ACAGCATCAG GTGCCCCAGA TGCTTCAAGA ACTCTCACTG 
GTAGTGAACC ATTGTAGGCT TCTTGGAGAG GAGATCGAAT TTTTAAAGCG ATGGGGGCCA 
AATTATAGCC TAATGCATAT AAATGTGAAT AATACTGAAT TGAGGCTTTT ATTCTCCAGT 
TGTGCAGCAT TTGCAAAGTT TGAGATAACT TTGTCTCCCT CGGCCCATTA TCCCTTGGTC 
CCGTTACCGT TCACCATTCA CAATCACATT GGGAAGACTG GCCATGATGA AATTGCTGCA 
ATTATCTCTA AAGTGCCCTT AGAGGAAAAC TACCTGAAGA ATGTAGTCAA GCAAATTTAC 
CAAGATCTAC TTAAGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Knl1 ( NM_029617.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_083893.2 Mus musculus kinetochore scaffold 1 (Knl1), transcript variant 1, mRNA.

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CloneID	OMu07220
Clone ID Related Accession (Same CDS sequence)	XM_006500376.2 , XM_006500377.4 , NM_029617.3 , XM_006500377.3 , NM_029617.2 , XM_006500376.3
Accession Version	NM_029617.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Mus musculus(house mouse)
Product	kinetochore scaffold 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL772264.8. On Apr 26, 2018 this sequence version replaced NM_029617.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164135 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGATGGGG TATATTCAGA AGCTAATGAA GAGAATGACA ATACACAGAG ACCTGTTAGA 
AGACAGCATT CCTCAATTTT GAAACCCCCA AGGAGTCCTC TTCAGGACCT CAAATGTGGA 
AATCAAACAA ACCAGGAACC TAATCCTCCA AGAAAAAGGA AAAGCTCTCG TCGAGTCAGC 
TTTGCAGATA CTATAAAAGT ATTCCAGACA GAGTCTCACA TGAAAACAGA GAGAAATTCA 
GAAATTTCAG GTATGAACAC TTTGCTTTGT GCTCCAATTC AGACTCAGAT GCAACAAAAG 
GAGTTCTCAA TTACAGACTG CAACCATGAA AGGAAACATG CAAATGATCA GACAGTCATC 
TTTTCAGATG AAAACCAGAT GGATCTGACA GCAAGTCACA CTGTAATGAT TACCAAAGGT 
CTTTCAGACT GTACCAAAAA TGAAAATTCT ACAAAAATAG ATACCACGTC CTTTCTGGAG 
AATTTAAAGC ATCATGCTGC AAATTCAAGA ATTAAAAAGG ATTTAGCATG TTCCACAGTT 
TCCTTAAGTC AAAACATTTT CTCAGAAAAG ATCAATTCTG ACAACTTCAT AAAAAGATTG 
AAGACAGGAA AACATATTTC GTCTTCTACA GAGCTTGATA AGGAAAATGC TGAGATACCT 
GTTTATTCCA AAGACTCAAA CAGTGCCTCT TCAACATATC AAATGCATGC ATCTCTTGGT 
GTAGATGAAA ATAGTAGTAA TAGGACTAGA ATCTTTAGAG AACAAGATGA TGGTATGAAT 
TTGACCCAAT GTCATACAGC CTGTATTAAG ACTTGGATTC CTCCATCCAC GGAGGCCAAA 
ATAGGAGAAT TCAAAGGTGA TAAAACAATT TATGGCAATG AGTGTATGGA GTTGACAACT 
AACTATACGA TACAGGTTTT GTCATCAGAA AATAATTTAT CTGAAAGAGA AACTCAAACT 
CAAAATGGTA TGAATGTTAC AACAGTTGAT GGAGCTACAG CTCCAGCTCC AGAGAAGAAA 
ACAGCCCTTA AGGATAAACT AAATGCTGCT TTTCAAGGCT CTTTCCCAAA CCCTGAAAAT 
AAAATTCATA TTATCAAATG TCATCCTATA GAGTCAGAAA CTCATACAGT CACACAGATT 
TCTAGTCAAA GTGCCAGCAC ATTGGCTGTA ACTTCAAAAT CTATATGTTC TAGTCCAGCT 
ATTGAGGGGT ATAAGACAAT TTTTCATTCT AGTAGCAATG ATGCAATGGA ACTGACTAAG 
TGTCTATCAG CTATGGAAGA GGAAAAAAAA TTGCTGAAGG CTGATGATAA GTATTCTAAA 
ATATGTACCA ATCCAGATGC TGGCCCACTC AGAGAGAAAA CTATTTATTT AGAAGAGGAT 
AGCATGGACA TTACCAAAAG TCACACCGTT GCAATAGATA ATAAAATATT TAAACATGAT 
CAAGAAAACA TTAAAAAAGA AATAATAGCC ATACCAATAT TTGAAAAGGA AATGGTGCTC 
CGAAATCTTA TGCCTATGTC AAAAGATGAG AAAAGGGATG TAAATTATAT TTCAGTTCCT 
CAAGTATCTA AGGAAAGCTT ACAACGGAGT CAGACAAACA CTTTATCTGT TTCATTGACT 
GACAAAAAGA TGGAATTCTT GGCAGATGAA GATATGGATC TGACTAAAAG TCACACAACT 
AAGTTAAGTC AAGTTATTCC TACAACTTTT GACTTAGCAT CAAAGAATGT CACCAAATCA 
TACTCTCACA GCAAAAGCCC TTTAAATGAG TGGGAAAGCC TGGATAAACA AGTAGTGCTA 
GGCCAGCATT CTAAACTTCC TTTACCACAG AGGAAAGATA GAGATGATCC TGACTGTTCT 
CATCATAAGA TAATGTATTC AGAGGAGTTA CAAACTATGG ATCTAACCAA GAGCCACACT 
ATTGTAATTG GCTTTGGTCC TTCTGAAGTA CAAGAACATA GTAAAATTAA TTTAGAACAC 
AAAAACAGCC AACTTACAGC AGAAAGCATA CAAACAGCTG TGAACGTTCC TGCAGCAAAC 
TCTAGAGTAG TAACAACTAA TGATATGGAC ATGTTAAAGG ACAGAAGTAC ACATAAGCCA 
GAATTGCTGA AGGAAAAGCA AAACATCAAA ATCTATGGAA GAAAAAGTAT TGGTAGACTG 
AAAATTGATA AAACTATATT ATTTTCAGAG GGTAATGAGG GTGATATGGA TATCACCAAG 
AGTTGCACAG TCAAAATAAA TCATAGATCT TTATTAGATA AGCATGATTC TCATTTAGTA 
TCTTTGGCAG GAACTTCTAA AACCATTTTG CATGCACGTG GTCAGGTTGA AATGGAAATC 
AATAGAAGTC ACACAACTCC TTTAGAATGT AAAATTATCT CGCCCAGTGA TATAACTCCT 
GGTGATCTGG ATAAGACTAT GATGTCTATA GATGATCATG AGGAACTAGA CATGACCAAG 
TCCCATACTG TTTTCATTGA CTACCAAGCA GAGGCAAAAG GTGTACTTCC AGACAGACTT 
GACTTTCAAT TATCCAAAAA GGAAAGCCTA CAGAAGCCGA AAGTGACATC TCTGGCTGAA 
GAGATTTACA TTTCAAAAAA TAGTGAAAGC AACCATCTAC CAGCAAAAGG CAGCCAGCTA 
ACAATATTAG AGGAAGGGTC TAATAGCGGG CTTGGAGAGG AAACTAATGA TGCACAAAAG 
CCTGGATTTC TGAATGAACT TCTGTCAGGC AAAACTCAGA GAAGAAAAAG CCTTAGCCTC 
AAAAACAAGA GTATTACATT TCCAGAGAAT GATAAAAGTT ATAGAGAGAT CCCTCAGAGT 
TCTGCAGTAG AAATAAATAA TGAAACTAGG CTGGAAGACA GAAAGGGCTT CAGTTTTGTG 
CCATTGGCAG GAACTTCCAA ACCAGTTTTG TCTGCATATG GGCCCGAGGA CATGGAGATT 
TCTATAGGTC AAACCACTGC TTCAGAATAT AAAACTGTCC CACCAGAAGA AATAACTACT 
ATACCTATGG ATAAAACTGT AATGTTTGTA GATAATTTCG GTGATCTGGA TGTCACCAGA 
TCCCATACGG TTTTCATCGA TTGTCAAGCC AAAGAAAAGG TACTTGATGA ATATACTAAC 
TTGGGAATAC AGAAAACAAA AACTTTGAGT GGTTCTGAAG GTGATACCCA TATTCAAGAA 
ATCACAAAAA ACCCAGCAGC TCAACACAAA CACCATATGA CAACTGTTAT TCCTTCTAGT 
ACAGTAGTTA GTGATCAAAG TTCAATGAAA ATCAAGTTTC ACAAAGCTGA TAGAGATGAG 
GAGGTCAAAG GAAAGGAAGT GGAAGCCAAT ATGCTGAAAC AAACTAAACC TGAAAGCTGT 
CTGTTAAATA TAACAGATGG AAAAAATGTG GATTTTACAA GCAGTTATAC AGCTGATGTT 
TGTAGATCCA GTGACAAGTA TTCCTCTTTA CCAAATATTT CTTCTTCTGA TAATTCGGGT 
GGTAATACTA TGTCCTTGTG TGATAAAAAT AAGGAGAAAG CTTATAATTG CCAAGTGCCA 
AATGAATTTA CTTATGCAGC TATCTTACCA AGTACATATC ACATGGATTC TAAAAAACTG 
TCTGTCTTTC CACCTTGTCC TTCAAAAGAA GTCACTCAAA CTGAAAGTGC CATAGCGCTG 
CTTAAAGATG AAGATCCGGT AGAAGAGCCT CTGGGGGAGA TGGCTACATT TAACTCAAAG 
CATGTATCTC TTAACCTGGC AAAGGATCAA ACAGAGGCCT TTGTTGATGT TAGTGTTGCT 
TCTCAGCCTC ATCTCTCAGC CCAACAAAGT CCATCAACTC AAAAAGGGCA GGATGTTGCC 
CGTAGAGATG AAGGAATATT AGCTAAAGCT GGGAAGAAGG CTTTACCTTT TCTTCTAGAA 
AATGTCGCTG CATCCACATG GGAAAATGAG TCCAAAATAC CCACTAATGT GGAACACTTT 
GCTGTAACCT ATGAAAAAGA ACTGAGTATA AGTATTCAAA CAGATAAATG TAATACAAAT 
GTACAGAGCC CTAGTAACTC GGCCCTGACA ACACAAGTCA TTCAGACGCA TGCAAATGCT 
GAAGGAGCAC TAGACTTTTT AGTTCCATCT ACTGTTTCAT GTTTTAGTAG TACCAAACCA 
AGTCTGAGTA ATTTGAATAG AAAAACTGAG GAGGTCTTAG ACTTTCAGAC TGTTAATTTA 
TTACCTCCTG CCGAACAGTT ACTTGAAGAA GGAAGTCAGG CACATAGTAT GAGTATAGTA 
CAAGCTACAG AAATATATAG ACTAGGTTCC AGGAATGATA GAGATGAAGA AAGTAAAACT 
TTCTGTAATG AGGCTGAAAC TACATCTGTA CCACTAAAAA CAGCTGTTAA AGACAAAACA 
AGAAGATGTT CATTGGGGAT ATTTTTACCC AAATTACCAA GCAAGAGAAG TTGTAGTATC 
ACTGGTGTTG ATGACCTGGA ACAGATTCTA GCAGATGCAG CTGACTTAAC TCAACTAGAA 
ACTCAGCCAG TCTGTAGTAA AGATCCAGGC ATTGGATCTG TTGCAGCCAA ACTGAATTTG 
AGTCCATCTC AGTTTATAAA TGAGGAAAAT CTCCCTGTAT ATCCTGGTGA GATACTTTCA 
TCAGATTCTG TTAGCCTGGA CATTGAGGAA AGTGTTCTGA TTGACACATC TCAGAGAGAG 
AGTTTACCGT CTGAAAACAA AACCGAAAAC TGCAGGGCCC AAAAAAGAAC CCGGGTTGAA 
GAAAATGATG TTACAAATGA GAAAAAAATC AGAACACATG ACAGTGCACA AGATCAAGAG 
ATTTTTGATA ATCATATGGA AGAGGATATC AATAAAAATG TCAACAGTGT ATTGCTGAAA 
AGCCTGAGCA GGACACCGTC TAGTTGCAGC AGCTCTCTGG ATTCAATCAA GTCTGATGGA 
CTCTCTCTGG ATGTCAGCAC ACAGCGTAAT AGCCAAATGG AATCACAGTT TCTTGGTGAC 
ACTATCTCTG AAGAGAGTTT GAAGGAGAAA CTCAAGGATG GACAAATTAC AATAAAGGAG 
TTCTTCATAC TTCTTCAGGT CCACATCTTA ATACAGAAAC CTAGGCAGAG CACTCTCCCA 
GCCAAATTTA CTATAAACAC ATTACCTACC ACAGAAGATC TGATGTTGAG ACAATATGTT 
TATGGACCCA GGATACAGAT TTATAAAGAA GACTGTGAGC TTCTTCGACA AAAGATTGAC 
GAATTAAAGA TATCTGCATT GAACCAAAAT AAACTATTGG CGGATGTAAA TAGGAACTTG 
TGGGAAAAAG TGAAAGACTA CTCTGATGAA GAGCTGAAGA ACTATGGGAT TTACCTTAAC 
AAAATCAAAT CACGTTATAC CAAGATGACA AAAGTCTTCA ATCACCAAGG AAAAGTGGCT 
CTGTATAACA AATTGGTGCA TTCAGCTGAG AATGAGAAGA ACAAACTTCA GATAAAAATA 
AATGAGATGG ACACTATCCT CAAGAAGATC AATAACTGTC TCGCTGAAGT GGAAACAGAA 
ACTAAGAATT TGGAGAATGA AGAGAAAAAT GATGCCATGG AAGAATGGGA TTCTGAAATG 
AGAGATGCAG AGAAAGAACT GGAACAGCTG AAAACTGAAG AAGAGGAGCT TCAGAGAAAG 
TTCTTAGAAG TAGAGACACA GAAAACGCAG ACCCTTGCTC AGATAGAATT TATAAAAGAA 
CAAACAACTA AAACTGAAGA ATTACTGGAT CAATTGAGTT TGTCTGAGTG GGATGTCATT 
GAGTGGAGTG ATGATCAAGC TGTATTTACC TTTGTCTATG ATTCAATAGA ACTCATCATC 
ACCTTTGGAG AGCCACTAGT TGGTCTCCCT TTTTTGGACA AGGCTTGCAG GAAGATTAAT 
GCACTCAGTT TTCAGTCTCT GTTAGATGAA GATAAAGCTC CTCCCTCCTC CCTTTTAGTT 
CACAAGCTTA TTTTTCAGTA CATTGAGGAA CAGGAATCCT GGAAGAAGAA ATGCACAGCA 
CAGCATCAGG TGCCCCAGAT GCTTCAAGAA CTCTCACTGG TAGTGAACCA TTGTAGGCTT 
CTTGGAGAGG AGATCGAATT TTTAAAGCGA TGGGGGCCAA ATTATAGCCT AATGCATATA 
AATGTGAATA ATACTGAATT GAGGCTTTTA TTCTCCAGTT GTGCAGCATT TGCAAAGTTT 
GAGATAACTT TGTCTCCCTC GGCCCATTAT CCCTTGGTCC CGTTACCGTT CACCATTCAC 
AATCACATTG GGAAGACTGG CCATGATGAA ATTGCTGCAA TTATCTCTAA AGTGCCCTTA 
GAGGAAAACT ACCTGAAGAA TGTAGTCAAG CAAATTTACC AAGATCTACT TAAGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_083893.2
CDS	122..6481
Translation	MDGVYSEANEENDNTQRPVRRQHSSILKPPRSPLQDLKCGNQTNQEPNPPRKRKSSRRVSFADTIKVFQTESHMKTERNSEISGMNTLLCAPIQTQMQQKEFSITDCNHERKHANDQTVIFSDENQMDLTASHTVMITKGLSDCTKNENSTKIDTTSFLENLKHHAANSRIKKDLACSTVSLSQNIFSEKINSDNFIKRLKTGKHISSSTELDKENAEIPVYSKDSNSASSTYQMHASLGVDENSSNRTRIFREQDDGMNLTQCHTACIKTWIPPSTEAKIGEFKGDKTIYGNECMELTTNYTIQVLSSENNLSERETQTQNGMNVTTVDGATAPAPEKKTALKDKLNAAFQGSFPNPENKIHIIKCHPIESETHTVTQISSQSASTLAVTSKSICSSPAIEGYKTIFHSSSNDAMELTKCLSAMEEEKKLLKADDKYSKICTNPDAGPLREKTIYLEEDSMDITKSHTVAIDNKIFKHDQENIKKEIIAIPIFEKEMVLRNLMPMSKDEKRDVNYISVPQVSKESLQRSQTNTLSVSLTDKKMEFLADEDMDLTKSHTTKLSQVIPTTFDLASKNVTKSYSHSKSPLNEWESLDKQVVLGQHSKLPLPQRKDRDDPDCSHHKIMYSEELQTMDLTKSHTIVIGFGPSEVQEHSKINLEHKNSQLTAESIQTAVNVPAANSRVVTTNDMDMLKDRSTHKPELLKEKQNIKIYGRKSIGRLKIDKTILFSEGNEGDMDITKSCTVKINHRSLLDKHDSHLVSLAGTSKTILHARGQVEMEINRSHTTPLECKIISPSDITPGDLDKTMMSIDDHEELDMTKSHTVFIDYQAEAKGVLPDRLDFQLSKKESLQKPKVTSLAEEIYISKNSESNHLPAKGSQLTILEEGSNSGLGEETNDAQKPGFLNELLSGKTQRRKSLSLKNKSITFPENDKSYREIPQSSAVEINNETRLEDRKGFSFVPLAGTSKPVLSAYGPEDMEISIGQTTASEYKTVPPEEITTIPMDKTVMFVDNFGDLDVTRSHTVFIDCQAKEKVLDEYTNLGIQKTKTLSGSEGDTHIQEITKNPAAQHKHHMTTVIPSSTVVSDQSSMKIKFHKADRDEEVKGKEVEANMLKQTKPESCLLNITDGKNVDFTSSYTADVCRSSDKYSSLPNISSSDNSGGNTMSLCDKNKEKAYNCQVPNEFTYAAILPSTYHMDSKKLSVFPPCPSKEVTQTESAIALLKDEDPVEEPLGEMATFNSKHVSLNLAKDQTEAFVDVSVASQPHLSAQQSPSTQKGQDVARRDEGILAKAGKKALPFLLENVAASTWENESKIPTNVEHFAVTYEKELSISIQTDKCNTNVQSPSNSALTTQVIQTHANAEGALDFLVPSTVSCFSSTKPSLSNLNRKTEEVLDFQTVNLLPPAEQLLEEGSQAHSMSIVQATEIYRLGSRNDRDEESKTFCNEAETTSVPLKTAVKDKTRRCSLGIFLPKLPSKRSCSITGVDDLEQILADAADLTQLETQPVCSKDPGIGSVAAKLNLSPSQFINEENLPVYPGEILSSDSVSLDIEESVLIDTSQRESLPSENKTENCRAQKRTRVEENDVTNEKKIRTHDSAQDQEIFDNHMEEDINKNVNSVLLKSLSRTPSSCSSSLDSIKSDGLSLDVSTQRNSQMESQFLGDTISEESLKEKLKDGQITIKEFFILLQVHILIQKPRQSTLPAKFTINTLPTTEDLMLRQYVYGPRIQIYKEDCELLRQKIDELKISALNQNKLLADVNRNLWEKVKDYSDEELKNYGIYLNKIKSRYTKMTKVFNHQGKVALYNKLVHSAENEKNKLQIKINEMDTILKKINNCLAEVETETKNLENEEKNDAMEEWDSEMRDAEKELEQLKTEEEELQRKFLEVETQKTQTLAQIEFIKEQTTKTEELLDQLSLSEWDVIEWSDDQAVFTFVYDSIELIITFGEPLVGLPFLDKACRKINALSFQSLLDEDKAPPSSLLVHKLIFQYIEEQESWKKKCTAQHQVPQMLQELSLVVNHCRLLGEEIEFLKRWGPNYSLMHINVNNTELRLLFSSCAAFAKFEITLSPSAHYPLVPLPFTIHNHIGKTGHDEIAAIISKVPLEENYLKNVVKQIYQDLLKD

Target ORF information:

RefSeq Version	NM_029617.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinetochore scaffold 1 (Knl1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_029617.3

ORF Insert Sequence:

ATGGATGGGG TATATTCAGA AGCTAATGAA GAGAATGACA ATACACAGAG ACCTGTTAGA 
AGACAGCATT CCTCAATTTT GAAACCCCCA AGGAGTCCTC TTCAGGACCT CAAATGTGGA 
AATCAAACAA ACCAGGAACC TAATCCTCCA AGAAAAAGGA AAAGCTCTCG TCGAGTCAGC 
TTTGCAGATA CTATAAAAGT ATTCCAGACA GAGTCTCACA TGAAAACAGA GAGAAATTCA 
GAAATTTCAG GTATGAACAC TTTGCTTTGT GCTCCAATTC AGACTCAGAT GCAACAAAAG 
GAGTTCTCAA TTACAGACTG CAACCATGAA AGGAAACATG CAAATGATCA GACAGTCATC 
TTTTCAGATG AAAACCAGAT GGATCTGACA GCAAGTCACA CTGTAATGAT TACCAAAGGT 
CTTTCAGACT GTACCAAAAA TGAAAATTCT ACAAAAATAG ATACCACGTC CTTTCTGGAG 
AATTTAAAGC ATCATGCTGC AAATTCAAGA ATTAAAAAGG ATTTAGCATG TTCCACAGTT 
TCCTTAAGTC AAAACATTTT CTCAGAAAAG ATCAATTCTG ACAACTTCAT AAAAAGATTG 
AAGACAGGAA AACATATTTC GTCTTCTACA GAGCTTGATA AGGAAAATGC TGAGATACCT 
GTTTATTCCA AAGACTCAAA CAGTGCCTCT TCAACATATC AAATGCATGC ATCTCTTGGT 
GTAGATGAAA ATAGTAGTAA TAGGACTAGA ATCTTTAGAG AACAAGATGA TGGTATGAAT 
TTGACCCAAT GTCATACAGC CTGTATTAAG ACTTGGATTC CTCCATCCAC GGAGGCCAAA 
ATAGGAGAAT TCAAAGGTGA TAAAACAATT TATGGCAATG AGTGTATGGA GTTGACAACT 
AACTATACGA TACAGGTTTT GTCATCAGAA AATAATTTAT CTGAAAGAGA AACTCAAACT 
CAAAATGGTA TGAATGTTAC AACAGTTGAT GGAGCTACAG CTCCAGCTCC AGAGAAGAAA 
ACAGCCCTTA AGGATAAACT AAATGCTGCT TTTCAAGGCT CTTTCCCAAA CCCTGAAAAT 
AAAATTCATA TTATCAAATG TCATCCTATA GAGTCAGAAA CTCATACAGT CACACAGATT 
TCTAGTCAAA GTGCCAGCAC ATTGGCTGTA ACTTCAAAAT CTATATGTTC TAGTCCAGCT 
ATTGAGGGGT ATAAGACAAT TTTTCATTCT AGTAGCAATG ATGCAATGGA ACTGACTAAG 
TGTCTATCAG CTATGGAAGA GGAAAAAAAA TTGCTGAAGG CTGATGATAA GTATTCTAAA 
ATATGTACCA ATCCAGATGC TGGCCCACTC AGAGAGAAAA CTATTTATTT AGAAGAGGAT 
AGCATGGACA TTACCAAAAG TCACACCGTT GCAATAGATA ATAAAATATT TAAACATGAT 
CAAGAAAACA TTAAAAAAGA AATAATAGCC ATACCAATAT TTGAAAAGGA AATGGTGCTC 
CGAAATCTTA TGCCTATGTC AAAAGATGAG AAAAGGGATG TAAATTATAT TTCAGTTCCT 
CAAGTATCTA AGGAAAGCTT ACAACGGAGT CAGACAAACA CTTTATCTGT TTCATTGACT 
GACAAAAAGA TGGAATTCTT GGCAGATGAA GATATGGATC TGACTAAAAG TCACACAACT 
AAGTTAAGTC AAGTTATTCC TACAACTTTT GACTTAGCAT CAAAGAATGT CACCAAATCA 
TACTCTCACA GCAAAAGCCC TTTAAATGAG TGGGAAAGCC TGGATAAACA AGTAGTGCTA 
GGCCAGCATT CTAAACTTCC TTTACCACAG AGGAAAGATA GAGATGATCC TGACTGTTCT 
CATCATAAGA TAATGTATTC AGAGGAGTTA CAAACTATGG ATCTAACCAA GAGCCACACT 
ATTGTAATTG GCTTTGGTCC TTCTGAAGTA CAAGAACATA GTAAAATTAA TTTAGAACAC 
AAAAACAGCC AACTTACAGC AGAAAGCATA CAAACAGCTG TGAACGTTCC TGCAGCAAAC 
TCTAGAGTAG TAACAACTAA TGATATGGAC ATGTTAAAGG ACAGAAGTAC ACATAAGCCA 
GAATTGCTGA AGGAAAAGCA AAACATCAAA ATCTATGGAA GAAAAAGTAT TGGTAGACTG 
AAAATTGATA AAACTATATT ATTTTCAGAG GGTAATGAGG GTGATATGGA TATCACCAAG 
AGTTGCACAG TCAAAATAAA TCATAGATCT TTATTAGATA AGCATGATTC TCATTTAGTA 
TCTTTGGCAG GAACTTCTAA AACCATTTTG CATGCACGTG GTCAGGTTGA AATGGAAATC 
AATAGAAGTC ACACAACTCC TTTAGAATGT AAAATTATCT CGCCCAGTGA TATAACTCCT 
GGTGATCTGG ATAAGACTAT GATGTCTATA GATGATCATG AGGAACTAGA CATGACCAAG 
TCCCATACTG TTTTCATTGA CTACCAAGCA GAGGCAAAAG GTGTACTTCC AGACAGACTT 
GACTTTCAAT TATCCAAAAA GGAAAGCCTA CAGAAGCCGA AAGTGACATC TCTGGCTGAA 
GAGATTTACA TTTCAAAAAA TAGTGAAAGC AACCATCTAC CAGCAAAAGG CAGCCAGCTA 
ACAATATTAG AGGAAGGGTC TAATAGCGGG CTTGGAGAGG AAACTAATGA TGCACAAAAG 
CCTGGATTTC TGAATGAACT TCTGTCAGGC AAAACTCAGA GAAGAAAAAG CCTTAGCCTC 
AAAAACAAGA GTATTACATT TCCAGAGAAT GATAAAAGTT ATAGAGAGAT CCCTCAGAGT 
TCTGCAGTAG AAATAAATAA TGAAACTAGG CTGGAAGACA GAAAGGGCTT CAGTTTTGTG 
CCATTGGCAG GAACTTCCAA ACCAGTTTTG TCTGCATATG GGCCCGAGGA CATGGAGATT 
TCTATAGGTC AAACCACTGC TTCAGAATAT AAAACTGTCC CACCAGAAGA AATAACTACT 
ATACCTATGG ATAAAACTGT AATGTTTGTA GATAATTTCG GTGATCTGGA TGTCACCAGA 
TCCCATACGG TTTTCATCGA TTGTCAAGCC AAAGAAAAGG TACTTGATGA ATATACTAAC 
TTGGGAATAC AGAAAACAAA AACTTTGAGT GGTTCTGAAG GTGATACCCA TATTCAAGAA 
ATCACAAAAA ACCCAGCAGC TCAACACAAA CACCATATGA CAACTGTTAT TCCTTCTAGT 
ACAGTAGTTA GTGATCAAAG TTCAATGAAA ATCAAGTTTC ACAAAGCTGA TAGAGATGAG 
GAGGTCAAAG GAAAGGAAGT GGAAGCCAAT ATGCTGAAAC AAACTAAACC TGAAAGCTGT 
CTGTTAAATA TAACAGATGG AAAAAATGTG GATTTTACAA GCAGTTATAC AGCTGATGTT 
TGTAGATCCA GTGACAAGTA TTCCTCTTTA CCAAATATTT CTTCTTCTGA TAATTCGGGT 
GGTAATACTA TGTCCTTGTG TGATAAAAAT AAGGAGAAAG CTTATAATTG CCAAGTGCCA 
AATGAATTTA CTTATGCAGC TATCTTACCA AGTACATATC ACATGGATTC TAAAAAACTG 
TCTGTCTTTC CACCTTGTCC TTCAAAAGAA GTCACTCAAA CTGAAAGTGC CATAGCGCTG 
CTTAAAGATG AAGATCCGGT AGAAGAGCCT CTGGGGGAGA TGGCTACATT TAACTCAAAG 
CATGTATCTC TTAACCTGGC AAAGGATCAA ACAGAGGCCT TTGTTGATGT TAGTGTTGCT 
TCTCAGCCTC ATCTCTCAGC CCAACAAAGT CCATCAACTC AAAAAGGGCA GGATGTTGCC 
CGTAGAGATG AAGGAATATT AGCTAAAGCT GGGAAGAAGG CTTTACCTTT TCTTCTAGAA 
AATGTCGCTG CATCCACATG GGAAAATGAG TCCAAAATAC CCACTAATGT GGAACACTTT 
GCTGTAACCT ATGAAAAAGA ACTGAGTATA AGTATTCAAA CAGATAAATG TAATACAAAT 
GTACAGAGCC CTAGTAACTC GGCCCTGACA ACACAAGTCA TTCAGACGCA TGCAAATGCT 
GAAGGAGCAC TAGACTTTTT AGTTCCATCT ACTGTTTCAT GTTTTAGTAG TACCAAACCA 
AGTCTGAGTA ATTTGAATAG AAAAACTGAG GAGGTCTTAG ACTTTCAGAC TGTTAATTTA 
TTACCTCCTG CCGAACAGTT ACTTGAAGAA GGAAGTCAGG CACATAGTAT GAGTATAGTA 
CAAGCTACAG AAATATATAG ACTAGGTTCC AGGAATGATA GAGATGAAGA AAGTAAAACT 
TTCTGTAATG AGGCTGAAAC TACATCTGTA CCACTAAAAA CAGCTGTTAA AGACAAAACA 
AGAAGATGTT CATTGGGGAT ATTTTTACCC AAATTACCAA GCAAGAGAAG TTGTAGTATC 
ACTGGTGTTG ATGACCTGGA ACAGATTCTA GCAGATGCAG CTGACTTAAC TCAACTAGAA 
ACTCAGCCAG TCTGTAGTAA AGATCCAGGC ATTGGATCTG TTGCAGCCAA ACTGAATTTG 
AGTCCATCTC AGTTTATAAA TGAGGAAAAT CTCCCTGTAT ATCCTGGTGA GATACTTTCA 
TCAGATTCTG TTAGCCTGGA CATTGAGGAA AGTGTTCTGA TTGACACATC TCAGAGAGAG 
AGTTTACCGT CTGAAAACAA AACCGAAAAC TGCAGGGCCC AAAAAAGAAC CCGGGTTGAA 
GAAAATGATG TTACAAATGA GAAAAAAATC AGAACACATG ACAGTGCACA AGATCAAGAG 
ATTTTTGATA ATCATATGGA AGAGGATATC AATAAAAATG TCAACAGTGT ATTGCTGAAA 
AGCCTGAGCA GGACACCGTC TAGTTGCAGC AGCTCTCTGG ATTCAATCAA GTCTGATGGA 
CTCTCTCTGG ATGTCAGCAC ACAGCGTAAT AGCCAAATGG AATCACAGTT TCTTGGTGAC 
ACTATCTCTG AAGAGAGTTT GAAGGAGAAA CTCAAGGATG GACAAATTAC AATAAAGGAG 
TTCTTCATAC TTCTTCAGGT CCACATCTTA ATACAGAAAC CTAGGCAGAG CACTCTCCCA 
GCCAAATTTA CTATAAACAC ATTACCTACC ACAGAAGATC TGATGTTGAG ACAATATGTT 
TATGGACCCA GGATACAGAT TTATAAAGAA GACTGTGAGC TTCTTCGACA AAAGATTGAC 
GAATTAAAGA TATCTGCATT GAACCAAAAT AAACTATTGG CGGATGTAAA TAGGAACTTG 
TGGGAAAAAG TGAAAGACTA CTCTGATGAA GAGCTGAAGA ACTATGGGAT TTACCTTAAC 
AAAATCAAAT CACGTTATAC CAAGATGACA AAAGTCTTCA ATCACCAAGG AAAAGTGGCT 
CTGTATAACA AATTGGTGCA TTCAGCTGAG AATGAGAAGA ACAAACTTCA GATAAAAATA 
AATGAGATGG ACACTATCCT CAAGAAGATC AATAACTGTC TCGCTGAAGT GGAAACAGAA 
ACTAAGAATT TGGAGAATGA AGAGAAAAAT GATGCCATGG AAGAATGGGA TTCTGAAATG 
AGAGATGCAG AGAAAGAACT GGAACAGCTG AAAACTGAAG AAGAGGAGCT TCAGAGAAAG 
TTCTTAGAAG TAGAGACACA GAAAACGCAG ACCCTTGCTC AGATAGAATT TATAAAAGAA 
CAAACAACTA AAACTGAAGA ATTACTGGAT CAATTGAGTT TGTCTGAGTG GGATGTCATT 
GAGTGGAGTG ATGATCAAGC TGTATTTACC TTTGTCTATG ATTCAATAGA ACTCATCATC 
ACCTTTGGAG AGCCACTAGT TGGTCTCCCT TTTTTGGACA AGGCTTGCAG GAAGATTAAT 
GCACTCAGTT TTCAGTCTCT GTTAGATGAA GATAAAGCTC CTCCCTCCTC CCTTTTAGTT 
CACAAGCTTA TTTTTCAGTA CATTGAGGAA CAGGAATCCT GGAAGAAGAA ATGCACAGCA 
CAGCATCAGG TGCCCCAGAT GCTTCAAGAA CTCTCACTGG TAGTGAACCA TTGTAGGCTT 
CTTGGAGAGG AGATCGAATT TTTAAAGCGA TGGGGGCCAA ATTATAGCCT AATGCATATA 
AATGTGAATA ATACTGAATT GAGGCTTTTA TTCTCCAGTT GTGCAGCATT TGCAAAGTTT 
GAGATAACTT TGTCTCCCTC GGCCCATTAT CCCTTGGTCC CGTTACCGTT CACCATTCAC 
AATCACATTG GGAAGACTGG CCATGATGAA ATTGCTGCAA TTATCTCTAA AGTGCCCTTA 
GAGGAAAACT ACCTGAAGAA TGTAGTCAAG CAAATTTACC AAGATCTACT TAAGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Knl1 ( NM_029617.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_083893.2 Mus musculus kinetochore scaffold 1 (Knl1), mRNA.

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CloneID	OMu07220
Clone ID Related Accession (Same CDS sequence)	XM_006500376.2 , XM_006500377.4 , NM_029617.3 , XM_006500377.3 , NM_029617.2 , XM_006500376.3
Accession Version	NM_029617.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Mus musculus(house mouse)
Product	kinetochore scaffold 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AL772264.8. On or before Feb 10, 2007 this sequence version replaced NM_001081042.1, NM_029617.1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164135 [ECO:0000348] ##Evidence-Data-END##

ATGGATGGGG TATATTCAGA AGCTAATGAA GAGAATGACA ATACACAGAG ACCTGTTAGA 
AGACAGCATT CCTCAATTTT GAAACCCCCA AGGAGTCCTC TTCAGGACCT CAAATGTGGA 
AATCAAACAA ACCAGGAACC TAATCCTCCA AGAAAAAGGA AAAGCTCTCG TCGAGTCAGC 
TTTGCAGATA CTATAAAAGT ATTCCAGACA GAGTCTCACA TGAAAACAGA GAGAAATTCA 
GAAATTTCAG GTATGAACAC TTTGCTTTGT GCTCCAATTC AGACTCAGAT GCAACAAAAG 
GAGTTCTCAA TTACAGACTG CAACCATGAA AGGAAACATG CAAATGATCA GACAGTCATC 
TTTTCAGATG AAAACCAGAT GGATCTGACA GCAAGTCACA CTGTAATGAT TACCAAAGGT 
CTTTCAGACT GTACCAAAAA TGAAAATTCT ACAAAAATAG ATACCACGTC CTTTCTGGAG 
AATTTAAAGC ATCATGCTGC AAATTCAAGA ATTAAAAAGG ATTTAGCATG TTCCACAGTT 
TCCTTAAGTC AAAACATTTT CTCAGAAAAG ATCAATTCTG ACAACTTCAT AAAAAGATTG 
AAGACAGGAA AACATATTTC GTCTTCTACA GAGCTTGATA AGGAAAATGC TGAGATACCT 
GTTTATTCCA AAGACTCAAA CAGTGCCTCT TCAACATATC AAATGCATGC ATCTCTTGGT 
GTAGATGAAA ATAGTAGTAA TAGGACTAGA ATCTTTAGAG AACAAGATGA TGGTATGAAT 
TTGACCCAAT GTCATACAGC CTGTATTAAG ACTTGGATTC CTCCATCCAC GGAGGCCAAA 
ATAGGAGAAT TCAAAGGTGA TAAAACAATT TATGGCAATG AGTGTATGGA GTTGACAACT 
AACTATACGA TACAGGTTTT GTCATCAGAA AATAATTTAT CTGAAAGAGA AACTCAAACT 
CAAAATGGTA TGAATGTTAC AACAGTTGAT GGAGCTACAG CTCCAGCTCC AGAGAAGAAA 
ACAGCCCTTA AGGATAAACT AAATGCTGCT TTTCAAGGCT CTTTCCCAAA CCCTGAAAAT 
AAAATTCATA TTATCAAATG TCATCCTATA GAGTCAGAAA CTCATACAGT CACACAGATT 
TCTAGTCAAA GTGCCAGCAC ATTGGCTGTA ACTTCAAAAT CTATATGTTC TAGTCCAGCT 
ATTGAGGGGT ATAAGACAAT TTTTCATTCT AGTAGCAATG ATGCAATGGA ACTGACTAAG 
TGTCTATCAG CTATGGAAGA GGAAAAAAAA TTGCTGAAGG CTGATGATAA GTATTCTAAA 
ATATGTACCA ATCCAGATGC TGGCCCACTC AGAGAGAAAA CTATTTATTT AGAAGAGGAT 
AGCATGGACA TTACCAAAAG TCACACCGTT GCAATAGATA ATAAAATATT TAAACATGAT 
CAAGAAAACA TTAAAAAAGA AATAATAGCC ATACCAATAT TTGAAAAGGA AATGGTGCTC 
CGAAATCTTA TGCCTATGTC AAAAGATGAG AAAAGGGATG TAAATTATAT TTCAGTTCCT 
CAAGTATCTA AGGAAAGCTT ACAACGGAGT CAGACAAACA CTTTATCTGT TTCATTGACT 
GACAAAAAGA TGGAATTCTT GGCAGATGAA GATATGGATC TGACTAAAAG TCACACAACT 
AAGTTAAGTC AAGTTATTCC TACAACTTTT GACTTAGCAT CAAAGAATGT CACCAAATCA 
TACTCTCACA GCAAAAGCCC TTTAAATGAG TGGGAAAGCC TGGATAAACA AGTAGTGCTA 
GGCCAGCATT CTAAACTTCC TTTACCACAG AGGAAAGATA GAGATGATCC TGACTGTTCT 
CATCATAAGA TAATGTATTC AGAGGAGTTA CAAACTATGG ATCTAACCAA GAGCCACACT 
ATTGTAATTG GCTTTGGTCC TTCTGAAGTA CAAGAACATA GTAAAATTAA TTTAGAACAC 
AAAAACAGCC AACTTACAGC AGAAAGCATA CAAACAGCTG TGAACGTTCC TGCAGCAAAC 
TCTAGAGTAG TAACAACTAA TGATATGGAC ATGTTAAAGG ACAGAAGTAC ACATAAGCCA 
GAATTGCTGA AGGAAAAGCA AAACATCAAA ATCTATGGAA GAAAAAGTAT TGGTAGACTG 
AAAATTGATA AAACTATATT ATTTTCAGAG GGTAATGAGG GTGATATGGA TATCACCAAG 
AGTTGCACAG TCAAAATAAA TCATAGATCT TTATTAGATA AGCATGATTC TCATTTAGTA 
TCTTTGGCAG GAACTTCTAA AACCATTTTG CATGCACGTG GTCAGGTTGA AATGGAAATC 
AATAGAAGTC ACACAACTCC TTTAGAATGT AAAATTATCT CGCCCAGTGA TATAACTCCT 
GGTGATCTGG ATAAGACTAT GATGTCTATA GATGATCATG AGGAACTAGA CATGACCAAG 
TCCCATACTG TTTTCATTGA CTACCAAGCA GAGGCAAAAG GTGTACTTCC AGACAGACTT 
GACTTTCAAT TATCCAAAAA GGAAAGCCTA CAGAAGCCGA AAGTGACATC TCTGGCTGAA 
GAGATTTACA TTTCAAAAAA TAGTGAAAGC AACCATCTAC CAGCAAAAGG CAGCCAGCTA 
ACAATATTAG AGGAAGGGTC TAATAGCGGG CTTGGAGAGG AAACTAATGA TGCACAAAAG 
CCTGGATTTC TGAATGAACT TCTGTCAGGC AAAACTCAGA GAAGAAAAAG CCTTAGCCTC 
AAAAACAAGA GTATTACATT TCCAGAGAAT GATAAAAGTT ATAGAGAGAT CCCTCAGAGT 
TCTGCAGTAG AAATAAATAA TGAAACTAGG CTGGAAGACA GAAAGGGCTT CAGTTTTGTG 
CCATTGGCAG GAACTTCCAA ACCAGTTTTG TCTGCATATG GGCCCGAGGA CATGGAGATT 
TCTATAGGTC AAACCACTGC TTCAGAATAT AAAACTGTCC CACCAGAAGA AATAACTACT 
ATACCTATGG ATAAAACTGT AATGTTTGTA GATAATTTCG GTGATCTGGA TGTCACCAGA 
TCCCATACGG TTTTCATCGA TTGTCAAGCC AAAGAAAAGG TACTTGATGA ATATACTAAC 
TTGGGAATAC AGAAAACAAA AACTTTGAGT GGTTCTGAAG GTGATACCCA TATTCAAGAA 
ATCACAAAAA ACCCAGCAGC TCAACACAAA CACCATATGA CAACTGTTAT TCCTTCTAGT 
ACAGTAGTTA GTGATCAAAG TTCAATGAAA ATCAAGTTTC ACAAAGCTGA TAGAGATGAG 
GAGGTCAAAG GAAAGGAAGT GGAAGCCAAT ATGCTGAAAC AAACTAAACC TGAAAGCTGT 
CTGTTAAATA TAACAGATGG AAAAAATGTG GATTTTACAA GCAGTTATAC AGCTGATGTT 
TGTAGATCCA GTGACAAGTA TTCCTCTTTA CCAAATATTT CTTCTTCTGA TAATTCGGGT 
GGTAATACTA TGTCCTTGTG TGATAAAAAT AAGGAGAAAG CTTATAATTG CCAAGTGCCA 
AATGAATTTA CTTATGCAGC TATCTTACCA AGTACATATC ACATGGATTC TAAAAAACTG 
TCTGTCTTTC CACCTTGTCC TTCAAAAGAA GTCACTCAAA CTGAAAGTGC CATAGCGCTG 
CTTAAAGATG AAGATCCGGT AGAAGAGCCT CTGGGGGAGA TGGCTACATT TAACTCAAAG 
CATGTATCTC TTAACCTGGC AAAGGATCAA ACAGAGGCCT TTGTTGATGT TAGTGTTGCT 
TCTCAGCCTC ATCTCTCAGC CCAACAAAGT CCATCAACTC AAAAAGGGCA GGATGTTGCC 
CGTAGAGATG AAGGAATATT AGCTAAAGCT GGGAAGAAGG CTTTACCTTT TCTTCTAGAA 
AATGTCGCTG CATCCACATG GGAAAATGAG TCCAAAATAC CCACTAATGT GGAACACTTT 
GCTGTAACCT ATGAAAAAGA ACTGAGTATA AGTATTCAAA CAGATAAATG TAATACAAAT 
GTACAGAGCC CTAGTAACTC GGCCCTGACA ACACAAGTCA TTCAGACGCA TGCAAATGCT 
GAAGGAGCAC TAGACTTTTT AGTTCCATCT ACTGTTTCAT GTTTTAGTAG TACCAAACCA 
AGTCTGAGTA ATTTGAATAG AAAAACTGAG GAGGTCTTAG ACTTTCAGAC TGTTAATTTA 
TTACCTCCTG CCGAACAGTT ACTTGAAGAA GGAAGTCAGG CACATAGTAT GAGTATAGTA 
CAAGCTACAG AAATATATAG ACTAGGTTCC AGGAATGATA GAGATGAAGA AAGTAAAACT 
TTCTGTAATG AGGCTGAAAC TACATCTGTA CCACTAAAAA CAGCTGTTAA AGACAAAACA 
AGAAGATGTT CATTGGGGAT ATTTTTACCC AAATTACCAA GCAAGAGAAG TTGTAGTATC 
ACTGGTGTTG ATGACCTGGA ACAGATTCTA GCAGATGCAG CTGACTTAAC TCAACTAGAA 
ACTCAGCCAG TCTGTAGTAA AGATCCAGGC ATTGGATCTG TTGCAGCCAA ACTGAATTTG 
AGTCCATCTC AGTTTATAAA TGAGGAAAAT CTCCCTGTAT ATCCTGGTGA GATACTTTCA 
TCAGATTCTG TTAGCCTGGA CATTGAGGAA AGTGTTCTGA TTGACACATC TCAGAGAGAG 
AGTTTACCGT CTGAAAACAA AACCGAAAAC TGCAGGGCCC AAAAAAGAAC CCGGGTTGAA 
GAAAATGATG TTACAAATGA GAAAAAAATC AGAACACATG ACAGTGCACA AGATCAAGAG 
ATTTTTGATA ATCATATGGA AGAGGATATC AATAAAAATG TCAACAGTGT ATTGCTGAAA 
AGCCTGAGCA GGACACCGTC TAGTTGCAGC AGCTCTCTGG ATTCAATCAA GTCTGATGGA 
CTCTCTCTGG ATGTCAGCAC ACAGCGTAAT AGCCAAATGG AATCACAGTT TCTTGGTGAC 
ACTATCTCTG AAGAGAGTTT GAAGGAGAAA CTCAAGGATG GACAAATTAC AATAAAGGAG 
TTCTTCATAC TTCTTCAGGT CCACATCTTA ATACAGAAAC CTAGGCAGAG CACTCTCCCA 
GCCAAATTTA CTATAAACAC ATTACCTACC ACAGAAGATC TGATGTTGAG ACAATATGTT 
TATGGACCCA GGATACAGAT TTATAAAGAA GACTGTGAGC TTCTTCGACA AAAGATTGAC 
GAATTAAAGA TATCTGCATT GAACCAAAAT AAACTATTGG CGGATGTAAA TAGGAACTTG 
TGGGAAAAAG TGAAAGACTA CTCTGATGAA GAGCTGAAGA ACTATGGGAT TTACCTTAAC 
AAAATCAAAT CACGTTATAC CAAGATGACA AAAGTCTTCA ATCACCAAGG AAAAGTGGCT 
CTGTATAACA AATTGGTGCA TTCAGCTGAG AATGAGAAGA ACAAACTTCA GATAAAAATA 
AATGAGATGG ACACTATCCT CAAGAAGATC AATAACTGTC TCGCTGAAGT GGAAACAGAA 
ACTAAGAATT TGGAGAATGA AGAGAAAAAT GATGCCATGG AAGAATGGGA TTCTGAAATG 
AGAGATGCAG AGAAAGAACT GGAACAGCTG AAAACTGAAG AAGAGGAGCT TCAGAGAAAG 
TTCTTAGAAG TAGAGACACA GAAAACGCAG ACCCTTGCTC AGATAGAATT TATAAAAGAA 
CAAACAACTA AAACTGAAGA ATTACTGGAT CAATTGAGTT TGTCTGAGTG GGATGTCATT 
GAGTGGAGTG ATGATCAAGC TGTATTTACC TTTGTCTATG ATTCAATAGA ACTCATCATC 
ACCTTTGGAG AGCCACTAGT TGGTCTCCCT TTTTTGGACA AGGCTTGCAG GAAGATTAAT 
GCACTCAGTT TTCAGTCTCT GTTAGATGAA GATAAAGCTC CTCCCTCCTC CCTTTTAGTT 
CACAAGCTTA TTTTTCAGTA CATTGAGGAA CAGGAATCCT GGAAGAAGAA ATGCACAGCA 
CAGCATCAGG TGCCCCAGAT GCTTCAAGAA CTCTCACTGG TAGTGAACCA TTGTAGGCTT 
CTTGGAGAGG AGATCGAATT TTTAAAGCGA TGGGGGCCAA ATTATAGCCT AATGCATATA 
AATGTGAATA ATACTGAATT GAGGCTTTTA TTCTCCAGTT GTGCAGCATT TGCAAAGTTT 
GAGATAACTT TGTCTCCCTC GGCCCATTAT CCCTTGGTCC CGTTACCGTT CACCATTCAC 
AATCACATTG GGAAGACTGG CCATGATGAA ATTGCTGCAA TTATCTCTAA AGTGCCCTTA 
GAGGAAAACT ACCTGAAGAA TGTAGTCAAG CAAATTTACC AAGATCTACT TAAGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_083893.2
CDS	122..6481
Translation	MDGVYSEANEENDNTQRPVRRQHSSILKPPRSPLQDLKCGNQTNQEPNPPRKRKSSRRVSFADTIKVFQTESHMKTERNSEISGMNTLLCAPIQTQMQQKEFSITDCNHERKHANDQTVIFSDENQMDLTASHTVMITKGLSDCTKNENSTKIDTTSFLENLKHHAANSRIKKDLACSTVSLSQNIFSEKINSDNFIKRLKTGKHISSSTELDKENAEIPVYSKDSNSASSTYQMHASLGVDENSSNRTRIFREQDDGMNLTQCHTACIKTWIPPSTEAKIGEFKGDKTIYGNECMELTTNYTIQVLSSENNLSERETQTQNGMNVTTVDGATAPAPEKKTALKDKLNAAFQGSFPNPENKIHIIKCHPIESETHTVTQISSQSASTLAVTSKSICSSPAIEGYKTIFHSSSNDAMELTKCLSAMEEEKKLLKADDKYSKICTNPDAGPLREKTIYLEEDSMDITKSHTVAIDNKIFKHDQENIKKEIIAIPIFEKEMVLRNLMPMSKDEKRDVNYISVPQVSKESLQRSQTNTLSVSLTDKKMEFLADEDMDLTKSHTTKLSQVIPTTFDLASKNVTKSYSHSKSPLNEWESLDKQVVLGQHSKLPLPQRKDRDDPDCSHHKIMYSEELQTMDLTKSHTIVIGFGPSEVQEHSKINLEHKNSQLTAESIQTAVNVPAANSRVVTTNDMDMLKDRSTHKPELLKEKQNIKIYGRKSIGRLKIDKTILFSEGNEGDMDITKSCTVKINHRSLLDKHDSHLVSLAGTSKTILHARGQVEMEINRSHTTPLECKIISPSDITPGDLDKTMMSIDDHEELDMTKSHTVFIDYQAEAKGVLPDRLDFQLSKKESLQKPKVTSLAEEIYISKNSESNHLPAKGSQLTILEEGSNSGLGEETNDAQKPGFLNELLSGKTQRRKSLSLKNKSITFPENDKSYREIPQSSAVEINNETRLEDRKGFSFVPLAGTSKPVLSAYGPEDMEISIGQTTASEYKTVPPEEITTIPMDKTVMFVDNFGDLDVTRSHTVFIDCQAKEKVLDEYTNLGIQKTKTLSGSEGDTHIQEITKNPAAQHKHHMTTVIPSSTVVSDQSSMKIKFHKADRDEEVKGKEVEANMLKQTKPESCLLNITDGKNVDFTSSYTADVCRSSDKYSSLPNISSSDNSGGNTMSLCDKNKEKAYNCQVPNEFTYAAILPSTYHMDSKKLSVFPPCPSKEVTQTESAIALLKDEDPVEEPLGEMATFNSKHVSLNLAKDQTEAFVDVSVASQPHLSAQQSPSTQKGQDVARRDEGILAKAGKKALPFLLENVAASTWENESKIPTNVEHFAVTYEKELSISIQTDKCNTNVQSPSNSALTTQVIQTHANAEGALDFLVPSTVSCFSSTKPSLSNLNRKTEEVLDFQTVNLLPPAEQLLEEGSQAHSMSIVQATEIYRLGSRNDRDEESKTFCNEAETTSVPLKTAVKDKTRRCSLGIFLPKLPSKRSCSITGVDDLEQILADAADLTQLETQPVCSKDPGIGSVAAKLNLSPSQFINEENLPVYPGEILSSDSVSLDIEESVLIDTSQRESLPSENKTENCRAQKRTRVEENDVTNEKKIRTHDSAQDQEIFDNHMEEDINKNVNSVLLKSLSRTPSSCSSSLDSIKSDGLSLDVSTQRNSQMESQFLGDTISEESLKEKLKDGQITIKEFFILLQVHILIQKPRQSTLPAKFTINTLPTTEDLMLRQYVYGPRIQIYKEDCELLRQKIDELKISALNQNKLLADVNRNLWEKVKDYSDEELKNYGIYLNKIKSRYTKMTKVFNHQGKVALYNKLVHSAENEKNKLQIKINEMDTILKKINNCLAEVETETKNLENEEKNDAMEEWDSEMRDAEKELEQLKTEEEELQRKFLEVETQKTQTLAQIEFIKEQTTKTEELLDQLSLSEWDVIEWSDDQAVFTFVYDSIELIITFGEPLVGLPFLDKACRKINALSFQSLLDEDKAPPSSLLVHKLIFQYIEEQESWKKKCTAQHQVPQMLQELSLVVNHCRLLGEEIEFLKRWGPNYSLMHINVNNTELRLLFSSCAAFAKFEITLSPSAHYPLVPLPFTIHNHIGKTGHDEIAAIISKVPLEENYLKNVVKQIYQDLLKD

Target ORF information:

RefSeq Version	NM_029617.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinetochore scaffold 1 (Knl1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_029617.2

ORF Insert Sequence:

ATGGATGGGG TATATTCAGA AGCTAATGAA GAGAATGACA ATACACAGAG ACCTGTTAGA 
AGACAGCATT CCTCAATTTT GAAACCCCCA AGGAGTCCTC TTCAGGACCT CAAATGTGGA 
AATCAAACAA ACCAGGAACC TAATCCTCCA AGAAAAAGGA AAAGCTCTCG TCGAGTCAGC 
TTTGCAGATA CTATAAAAGT ATTCCAGACA GAGTCTCACA TGAAAACAGA GAGAAATTCA 
GAAATTTCAG GTATGAACAC TTTGCTTTGT GCTCCAATTC AGACTCAGAT GCAACAAAAG 
GAGTTCTCAA TTACAGACTG CAACCATGAA AGGAAACATG CAAATGATCA GACAGTCATC 
TTTTCAGATG AAAACCAGAT GGATCTGACA GCAAGTCACA CTGTAATGAT TACCAAAGGT 
CTTTCAGACT GTACCAAAAA TGAAAATTCT ACAAAAATAG ATACCACGTC CTTTCTGGAG 
AATTTAAAGC ATCATGCTGC AAATTCAAGA ATTAAAAAGG ATTTAGCATG TTCCACAGTT 
TCCTTAAGTC AAAACATTTT CTCAGAAAAG ATCAATTCTG ACAACTTCAT AAAAAGATTG 
AAGACAGGAA AACATATTTC GTCTTCTACA GAGCTTGATA AGGAAAATGC TGAGATACCT 
GTTTATTCCA AAGACTCAAA CAGTGCCTCT TCAACATATC AAATGCATGC ATCTCTTGGT 
GTAGATGAAA ATAGTAGTAA TAGGACTAGA ATCTTTAGAG AACAAGATGA TGGTATGAAT 
TTGACCCAAT GTCATACAGC CTGTATTAAG ACTTGGATTC CTCCATCCAC GGAGGCCAAA 
ATAGGAGAAT TCAAAGGTGA TAAAACAATT TATGGCAATG AGTGTATGGA GTTGACAACT 
AACTATACGA TACAGGTTTT GTCATCAGAA AATAATTTAT CTGAAAGAGA AACTCAAACT 
CAAAATGGTA TGAATGTTAC AACAGTTGAT GGAGCTACAG CTCCAGCTCC AGAGAAGAAA 
ACAGCCCTTA AGGATAAACT AAATGCTGCT TTTCAAGGCT CTTTCCCAAA CCCTGAAAAT 
AAAATTCATA TTATCAAATG TCATCCTATA GAGTCAGAAA CTCATACAGT CACACAGATT 
TCTAGTCAAA GTGCCAGCAC ATTGGCTGTA ACTTCAAAAT CTATATGTTC TAGTCCAGCT 
ATTGAGGGGT ATAAGACAAT TTTTCATTCT AGTAGCAATG ATGCAATGGA ACTGACTAAG 
TGTCTATCAG CTATGGAAGA GGAAAAAAAA TTGCTGAAGG CTGATGATAA GTATTCTAAA 
ATATGTACCA ATCCAGATGC TGGCCCACTC AGAGAGAAAA CTATTTATTT AGAAGAGGAT 
AGCATGGACA TTACCAAAAG TCACACCGTT GCAATAGATA ATAAAATATT TAAACATGAT 
CAAGAAAACA TTAAAAAAGA AATAATAGCC ATACCAATAT TTGAAAAGGA AATGGTGCTC 
CGAAATCTTA TGCCTATGTC AAAAGATGAG AAAAGGGATG TAAATTATAT TTCAGTTCCT 
CAAGTATCTA AGGAAAGCTT ACAACGGAGT CAGACAAACA CTTTATCTGT TTCATTGACT 
GACAAAAAGA TGGAATTCTT GGCAGATGAA GATATGGATC TGACTAAAAG TCACACAACT 
AAGTTAAGTC AAGTTATTCC TACAACTTTT GACTTAGCAT CAAAGAATGT CACCAAATCA 
TACTCTCACA GCAAAAGCCC TTTAAATGAG TGGGAAAGCC TGGATAAACA AGTAGTGCTA 
GGCCAGCATT CTAAACTTCC TTTACCACAG AGGAAAGATA GAGATGATCC TGACTGTTCT 
CATCATAAGA TAATGTATTC AGAGGAGTTA CAAACTATGG ATCTAACCAA GAGCCACACT 
ATTGTAATTG GCTTTGGTCC TTCTGAAGTA CAAGAACATA GTAAAATTAA TTTAGAACAC 
AAAAACAGCC AACTTACAGC AGAAAGCATA CAAACAGCTG TGAACGTTCC TGCAGCAAAC 
TCTAGAGTAG TAACAACTAA TGATATGGAC ATGTTAAAGG ACAGAAGTAC ACATAAGCCA 
GAATTGCTGA AGGAAAAGCA AAACATCAAA ATCTATGGAA GAAAAAGTAT TGGTAGACTG 
AAAATTGATA AAACTATATT ATTTTCAGAG GGTAATGAGG GTGATATGGA TATCACCAAG 
AGTTGCACAG TCAAAATAAA TCATAGATCT TTATTAGATA AGCATGATTC TCATTTAGTA 
TCTTTGGCAG GAACTTCTAA AACCATTTTG CATGCACGTG GTCAGGTTGA AATGGAAATC 
AATAGAAGTC ACACAACTCC TTTAGAATGT AAAATTATCT CGCCCAGTGA TATAACTCCT 
GGTGATCTGG ATAAGACTAT GATGTCTATA GATGATCATG AGGAACTAGA CATGACCAAG 
TCCCATACTG TTTTCATTGA CTACCAAGCA GAGGCAAAAG GTGTACTTCC AGACAGACTT 
GACTTTCAAT TATCCAAAAA GGAAAGCCTA CAGAAGCCGA AAGTGACATC TCTGGCTGAA 
GAGATTTACA TTTCAAAAAA TAGTGAAAGC AACCATCTAC CAGCAAAAGG CAGCCAGCTA 
ACAATATTAG AGGAAGGGTC TAATAGCGGG CTTGGAGAGG AAACTAATGA TGCACAAAAG 
CCTGGATTTC TGAATGAACT TCTGTCAGGC AAAACTCAGA GAAGAAAAAG CCTTAGCCTC 
AAAAACAAGA GTATTACATT TCCAGAGAAT GATAAAAGTT ATAGAGAGAT CCCTCAGAGT 
TCTGCAGTAG AAATAAATAA TGAAACTAGG CTGGAAGACA GAAAGGGCTT CAGTTTTGTG 
CCATTGGCAG GAACTTCCAA ACCAGTTTTG TCTGCATATG GGCCCGAGGA CATGGAGATT 
TCTATAGGTC AAACCACTGC TTCAGAATAT AAAACTGTCC CACCAGAAGA AATAACTACT 
ATACCTATGG ATAAAACTGT AATGTTTGTA GATAATTTCG GTGATCTGGA TGTCACCAGA 
TCCCATACGG TTTTCATCGA TTGTCAAGCC AAAGAAAAGG TACTTGATGA ATATACTAAC 
TTGGGAATAC AGAAAACAAA AACTTTGAGT GGTTCTGAAG GTGATACCCA TATTCAAGAA 
ATCACAAAAA ACCCAGCAGC TCAACACAAA CACCATATGA CAACTGTTAT TCCTTCTAGT 
ACAGTAGTTA GTGATCAAAG TTCAATGAAA ATCAAGTTTC ACAAAGCTGA TAGAGATGAG 
GAGGTCAAAG GAAAGGAAGT GGAAGCCAAT ATGCTGAAAC AAACTAAACC TGAAAGCTGT 
CTGTTAAATA TAACAGATGG AAAAAATGTG GATTTTACAA GCAGTTATAC AGCTGATGTT 
TGTAGATCCA GTGACAAGTA TTCCTCTTTA CCAAATATTT CTTCTTCTGA TAATTCGGGT 
GGTAATACTA TGTCCTTGTG TGATAAAAAT AAGGAGAAAG CTTATAATTG CCAAGTGCCA 
AATGAATTTA CTTATGCAGC TATCTTACCA AGTACATATC ACATGGATTC TAAAAAACTG 
TCTGTCTTTC CACCTTGTCC TTCAAAAGAA GTCACTCAAA CTGAAAGTGC CATAGCGCTG 
CTTAAAGATG AAGATCCGGT AGAAGAGCCT CTGGGGGAGA TGGCTACATT TAACTCAAAG 
CATGTATCTC TTAACCTGGC AAAGGATCAA ACAGAGGCCT TTGTTGATGT TAGTGTTGCT 
TCTCAGCCTC ATCTCTCAGC CCAACAAAGT CCATCAACTC AAAAAGGGCA GGATGTTGCC 
CGTAGAGATG AAGGAATATT AGCTAAAGCT GGGAAGAAGG CTTTACCTTT TCTTCTAGAA 
AATGTCGCTG CATCCACATG GGAAAATGAG TCCAAAATAC CCACTAATGT GGAACACTTT 
GCTGTAACCT ATGAAAAAGA ACTGAGTATA AGTATTCAAA CAGATAAATG TAATACAAAT 
GTACAGAGCC CTAGTAACTC GGCCCTGACA ACACAAGTCA TTCAGACGCA TGCAAATGCT 
GAAGGAGCAC TAGACTTTTT AGTTCCATCT ACTGTTTCAT GTTTTAGTAG TACCAAACCA 
AGTCTGAGTA ATTTGAATAG AAAAACTGAG GAGGTCTTAG ACTTTCAGAC TGTTAATTTA 
TTACCTCCTG CCGAACAGTT ACTTGAAGAA GGAAGTCAGG CACATAGTAT GAGTATAGTA 
CAAGCTACAG AAATATATAG ACTAGGTTCC AGGAATGATA GAGATGAAGA AAGTAAAACT 
TTCTGTAATG AGGCTGAAAC TACATCTGTA CCACTAAAAA CAGCTGTTAA AGACAAAACA 
AGAAGATGTT CATTGGGGAT ATTTTTACCC AAATTACCAA GCAAGAGAAG TTGTAGTATC 
ACTGGTGTTG ATGACCTGGA ACAGATTCTA GCAGATGCAG CTGACTTAAC TCAACTAGAA 
ACTCAGCCAG TCTGTAGTAA AGATCCAGGC ATTGGATCTG TTGCAGCCAA ACTGAATTTG 
AGTCCATCTC AGTTTATAAA TGAGGAAAAT CTCCCTGTAT ATCCTGGTGA GATACTTTCA 
TCAGATTCTG TTAGCCTGGA CATTGAGGAA AGTGTTCTGA TTGACACATC TCAGAGAGAG 
AGTTTACCGT CTGAAAACAA AACCGAAAAC TGCAGGGCCC AAAAAAGAAC CCGGGTTGAA 
GAAAATGATG TTACAAATGA GAAAAAAATC AGAACACATG ACAGTGCACA AGATCAAGAG 
ATTTTTGATA ATCATATGGA AGAGGATATC AATAAAAATG TCAACAGTGT ATTGCTGAAA 
AGCCTGAGCA GGACACCGTC TAGTTGCAGC AGCTCTCTGG ATTCAATCAA GTCTGATGGA 
CTCTCTCTGG ATGTCAGCAC ACAGCGTAAT AGCCAAATGG AATCACAGTT TCTTGGTGAC 
ACTATCTCTG AAGAGAGTTT GAAGGAGAAA CTCAAGGATG GACAAATTAC AATAAAGGAG 
TTCTTCATAC TTCTTCAGGT CCACATCTTA ATACAGAAAC CTAGGCAGAG CACTCTCCCA 
GCCAAATTTA CTATAAACAC ATTACCTACC ACAGAAGATC TGATGTTGAG ACAATATGTT 
TATGGACCCA GGATACAGAT TTATAAAGAA GACTGTGAGC TTCTTCGACA AAAGATTGAC 
GAATTAAAGA TATCTGCATT GAACCAAAAT AAACTATTGG CGGATGTAAA TAGGAACTTG 
TGGGAAAAAG TGAAAGACTA CTCTGATGAA GAGCTGAAGA ACTATGGGAT TTACCTTAAC 
AAAATCAAAT CACGTTATAC CAAGATGACA AAAGTCTTCA ATCACCAAGG AAAAGTGGCT 
CTGTATAACA AATTGGTGCA TTCAGCTGAG AATGAGAAGA ACAAACTTCA GATAAAAATA 
AATGAGATGG ACACTATCCT CAAGAAGATC AATAACTGTC TCGCTGAAGT GGAAACAGAA 
ACTAAGAATT TGGAGAATGA AGAGAAAAAT GATGCCATGG AAGAATGGGA TTCTGAAATG 
AGAGATGCAG AGAAAGAACT GGAACAGCTG AAAACTGAAG AAGAGGAGCT TCAGAGAAAG 
TTCTTAGAAG TAGAGACACA GAAAACGCAG ACCCTTGCTC AGATAGAATT TATAAAAGAA 
CAAACAACTA AAACTGAAGA ATTACTGGAT CAATTGAGTT TGTCTGAGTG GGATGTCATT 
GAGTGGAGTG ATGATCAAGC TGTATTTACC TTTGTCTATG ATTCAATAGA ACTCATCATC 
ACCTTTGGAG AGCCACTAGT TGGTCTCCCT TTTTTGGACA AGGCTTGCAG GAAGATTAAT 
GCACTCAGTT TTCAGTCTCT GTTAGATGAA GATAAAGCTC CTCCCTCCTC CCTTTTAGTT 
CACAAGCTTA TTTTTCAGTA CATTGAGGAA CAGGAATCCT GGAAGAAGAA ATGCACAGCA 
CAGCATCAGG TGCCCCAGAT GCTTCAAGAA CTCTCACTGG TAGTGAACCA TTGTAGGCTT 
CTTGGAGAGG AGATCGAATT TTTAAAGCGA TGGGGGCCAA ATTATAGCCT AATGCATATA 
AATGTGAATA ATACTGAATT GAGGCTTTTA TTCTCCAGTT GTGCAGCATT TGCAAAGTTT 
GAGATAACTT TGTCTCCCTC GGCCCATTAT CCCTTGGTCC CGTTACCGTT CACCATTCAC 
AATCACATTG GGAAGACTGG CCATGATGAA ATTGCTGCAA TTATCTCTAA AGTGCCCTTA 
GAGGAAAACT ACCTGAAGAA TGTAGTCAAG CAAATTTACC AAGATCTACT TAAGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Knl1 ( XM_006500376.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006500439.1 Mus musculus cancer susceptibility candidate 5 (Casc5), transcript variant X1, mRNA.

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CloneID	OMu07220
Clone ID Related Accession (Same CDS sequence)	XM_006500376.2 , XM_006500377.4 , NM_029617.3 , XM_006500377.3 , NM_029617.2 , XM_006500376.3
Accession Version	XM_006500376.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein CASC5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006500376.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGGGG TATATTCAGA AGCTAATGAA GAGAATGACA ATACACAGAG ACCTGTTAGA 
AGACAGCATT CCTCAATTTT GAAACCCCCA AGGAGTCCTC TTCAGGACCT CAAATGTGGA 
AATCAAACAA ACCAGGAACC TAATCCTCCA AGAAAAAGGA AAAGCTCTCG TCGAGTCAGC 
TTTGCAGATA CTATAAAAGT ATTCCAGACA GAGTCTCACA TGAAAACAGA GAGAAATTCA 
GAAATTTCAG GTATGAACAC TTTGCTTTGT GCTCCAATTC AGACTCAGAT GCAACAAAAG 
GAGTTCTCAA TTACAGACTG CAACCATGAA AGGAAACATG CAAATGATCA GACAGTCATC 
TTTTCAGATG AAAACCAGAT GGATCTGACA GCAAGTCACA CTGTAATGAT TACCAAAGGT 
CTTTCAGACT GTACCAAAAA TGAAAATTCT ACAAAAATAG ATACCACGTC CTTTCTGGAG 
AATTTAAAGC ATCATGCTGC AAATTCAAGA ATTAAAAAGG ATTTAGCATG TTCCACAGTT 
TCCTTAAGTC AAAACATTTT CTCAGAAAAG ATCAATTCTG ACAACTTCAT AAAAAGATTG 
AAGACAGGAA AACATATTTC GTCTTCTACA GAGCTTGATA AGGAAAATGC TGAGATACCT 
GTTTATTCCA AAGACTCAAA CAGTGCCTCT TCAACATATC AAATGCATGC ATCTCTTGGT 
GTAGATGAAA ATAGTAGTAA TAGGACTAGA ATCTTTAGAG AACAAGATGA TGGTATGAAT 
TTGACCCAAT GTCATACAGC CTGTATTAAG ACTTGGATTC CTCCATCCAC GGAGGCCAAA 
ATAGGAGAAT TCAAAGGTGA TAAAACAATT TATGGCAATG AGTGTATGGA GTTGACAACT 
AACTATACGA TACAGGTTTT GTCATCAGAA AATAATTTAT CTGAAAGAGA AACTCAAACT 
CAAAATGGTA TGAATGTTAC AACAGTTGAT GGAGCTACAG CTCCAGCTCC AGAGAAGAAA 
ACAGCCCTTA AGGATAAACT AAATGCTGCT TTTCAAGGCT CTTTCCCAAA CCCTGAAAAT 
AAAATTCATA TTATCAAATG TCATCCTATA GAGTCAGAAA CTCATACAGT CACACAGATT 
TCTAGTCAAA GTGCCAGCAC ATTGGCTGTA ACTTCAAAAT CTATATGTTC TAGTCCAGCT 
ATTGAGGGGT ATAAGACAAT TTTTCATTCT AGTAGCAATG ATGCAATGGA ACTGACTAAG 
TGTCTATCAG CTATGGAAGA GGAAAAAAAA TTGCTGAAGG CTGATGATAA GTATTCTAAA 
ATATGTACCA ATCCAGATGC TGGCCCACTC AGAGAGAAAA CTATTTATTT AGAAGAGGAT 
AGCATGGACA TTACCAAAAG TCACACCGTT GCAATAGATA ATAAAATATT TAAACATGAT 
CAAGAAAACA TTAAAAAAGA AATAATAGCC ATACCAATAT TTGAAAAGGA AATGGTGCTC 
CGAAATCTTA TGCCTATGTC AAAAGATGAG AAAAGGGATG TAAATTATAT TTCAGTTCCT 
CAAGTATCTA AGGAAAGCTT ACAACGGAGT CAGACAAACA CTTTATCTGT TTCATTGACT 
GACAAAAAGA TGGAATTCTT GGCAGATGAA GATATGGATC TGACTAAAAG TCACACAACT 
AAGTTAAGTC AAGTTATTCC TACAACTTTT GACTTAGCAT CAAAGAATGT CACCAAATCA 
TACTCTCACA GCAAAAGCCC TTTAAATGAG TGGGAAAGCC TGGATAAACA AGTAGTGCTA 
GGCCAGCATT CTAAACTTCC TTTACCACAG AGGAAAGATA GAGATGATCC TGACTGTTCT 
CATCATAAGA TAATGTATTC AGAGGAGTTA CAAACTATGG ATCTAACCAA GAGCCACACT 
ATTGTAATTG GCTTTGGTCC TTCTGAAGTA CAAGAACATA GTAAAATTAA TTTAGAACAC 
AAAAACAGCC AACTTACAGC AGAAAGCATA CAAACAGCTG TGAACGTTCC TGCAGCAAAC 
TCTAGAGTAG TAACAACTAA TGATATGGAC ATGTTAAAGG ACAGAAGTAC ACATAAGCCA 
GAATTGCTGA AGGAAAAGCA AAACATCAAA ATCTATGGAA GAAAAAGTAT TGGTAGACTG 
AAAATTGATA AAACTATATT ATTTTCAGAG GGTAATGAGG GTGATATGGA TATCACCAAG 
AGTTGCACAG TCAAAATAAA TCATAGATCT TTATTAGATA AGCATGATTC TCATTTAGTA 
TCTTTGGCAG GAACTTCTAA AACCATTTTG CATGCACGTG GTCAGGTTGA AATGGAAATC 
AATAGAAGTC ACACAACTCC TTTAGAATGT AAAATTATCT CGCCCAGTGA TATAACTCCT 
GGTGATCTGG ATAAGACTAT GATGTCTATA GATGATCATG AGGAACTAGA CATGACCAAG 
TCCCATACTG TTTTCATTGA CTACCAAGCA GAGGCAAAAG GTGTACTTCC AGACAGACTT 
GACTTTCAAT TATCCAAAAA GGAAAGCCTA CAGAAGCCGA AAGTGACATC TCTGGCTGAA 
GAGATTTACA TTTCAAAAAA TAGTGAAAGC AACCATCTAC CAGCAAAAGG CAGCCAGCTA 
ACAATATTAG AGGAAGGGTC TAATAGCGGG CTTGGAGAGG AAACTAATGA TGCACAAAAG 
CCTGGATTTC TGAATGAACT TCTGTCAGGC AAAACTCAGA GAAGAAAAAG CCTTAGCCTC 
AAAAACAAGA GTATTACATT TCCAGAGAAT GATAAAAGTT ATAGAGAGAT CCCTCAGAGT 
TCTGCAGTAG AAATAAATAA TGAAACTAGG CTGGAAGACA GAAAGGGCTT CAGTTTTGTG 
CCATTGGCAG GAACTTCCAA ACCAGTTTTG TCTGCATATG GGCCCGAGGA CATGGAGATT 
TCTATAGGTC AAACCACTGC TTCAGAATAT AAAACTGTCC CACCAGAAGA AATAACTACT 
ATACCTATGG ATAAAACTGT AATGTTTGTA GATAATTTCG GTGATCTGGA TGTCACCAGA 
TCCCATACGG TTTTCATCGA TTGTCAAGCC AAAGAAAAGG TACTTGATGA ATATACTAAC 
TTGGGAATAC AGAAAACAAA AACTTTGAGT GGTTCTGAAG GTGATACCCA TATTCAAGAA 
ATCACAAAAA ACCCAGCAGC TCAACACAAA CACCATATGA CAACTGTTAT TCCTTCTAGT 
ACAGTAGTTA GTGATCAAAG TTCAATGAAA ATCAAGTTTC ACAAAGCTGA TAGAGATGAG 
GAGGTCAAAG GAAAGGAAGT GGAAGCCAAT ATGCTGAAAC AAACTAAACC TGAAAGCTGT 
CTGTTAAATA TAACAGATGG AAAAAATGTG GATTTTACAA GCAGTTATAC AGCTGATGTT 
TGTAGATCCA GTGACAAGTA TTCCTCTTTA CCAAATATTT CTTCTTCTGA TAATTCGGGT 
GGTAATACTA TGTCCTTGTG TGATAAAAAT AAGGAGAAAG CTTATAATTG CCAAGTGCCA 
AATGAATTTA CTTATGCAGC TATCTTACCA AGTACATATC ACATGGATTC TAAAAAACTG 
TCTGTCTTTC CACCTTGTCC TTCAAAAGAA GTCACTCAAA CTGAAAGTGC CATAGCGCTG 
CTTAAAGATG AAGATCCGGT AGAAGAGCCT CTGGGGGAGA TGGCTACATT TAACTCAAAG 
CATGTATCTC TTAACCTGGC AAAGGATCAA ACAGAGGCCT TTGTTGATGT TAGTGTTGCT 
TCTCAGCCTC ATCTCTCAGC CCAACAAAGT CCATCAACTC AAAAAGGGCA GGATGTTGCC 
CGTAGAGATG AAGGAATATT AGCTAAAGCT GGGAAGAAGG CTTTACCTTT TCTTCTAGAA 
AATGTCGCTG CATCCACATG GGAAAATGAG TCCAAAATAC CCACTAATGT GGAACACTTT 
GCTGTAACCT ATGAAAAAGA ACTGAGTATA AGTATTCAAA CAGATAAATG TAATACAAAT 
GTACAGAGCC CTAGTAACTC GGCCCTGACA ACACAAGTCA TTCAGACGCA TGCAAATGCT 
GAAGGAGCAC TAGACTTTTT AGTTCCATCT ACTGTTTCAT GTTTTAGTAG TACCAAACCA 
AGTCTGAGTA ATTTGAATAG AAAAACTGAG GAGGTCTTAG ACTTTCAGAC TGTTAATTTA 
TTACCTCCTG CCGAACAGTT ACTTGAAGAA GGAAGTCAGG CACATAGTAT GAGTATAGTA 
CAAGCTACAG AAATATATAG ACTAGGTTCC AGGAATGATA GAGATGAAGA AAGTAAAACT 
TTCTGTAATG AGGCTGAAAC TACATCTGTA CCACTAAAAA CAGCTGTTAA AGACAAAACA 
AGAAGATGTT CATTGGGGAT ATTTTTACCC AAATTACCAA GCAAGAGAAG TTGTAGTATC 
ACTGGTGTTG ATGACCTGGA ACAGATTCTA GCAGATGCAG CTGACTTAAC TCAACTAGAA 
ACTCAGCCAG TCTGTAGTAA AGATCCAGGC ATTGGATCTG TTGCAGCCAA ACTGAATTTG 
AGTCCATCTC AGTTTATAAA TGAGGAAAAT CTCCCTGTAT ATCCTGGTGA GATACTTTCA 
TCAGATTCTG TTAGCCTGGA CATTGAGGAA AGTGTTCTGA TTGACACATC TCAGAGAGAG 
AGTTTACCGT CTGAAAACAA AACCGAAAAC TGCAGGGCCC AAAAAAGAAC CCGGGTTGAA 
GAAAATGATG TTACAAATGA GAAAAAAATC AGAACACATG ACAGTGCACA AGATCAAGAG 
ATTTTTGATA ATCATATGGA AGAGGATATC AATAAAAATG TCAACAGTGT ATTGCTGAAA 
AGCCTGAGCA GGACACCGTC TAGTTGCAGC AGCTCTCTGG ATTCAATCAA GTCTGATGGA 
CTCTCTCTGG ATGTCAGCAC ACAGCGTAAT AGCCAAATGG AATCACAGTT TCTTGGTGAC 
ACTATCTCTG AAGAGAGTTT GAAGGAGAAA CTCAAGGATG GACAAATTAC AATAAAGGAG 
TTCTTCATAC TTCTTCAGGT CCACATCTTA ATACAGAAAC CTAGGCAGAG CACTCTCCCA 
GCCAAATTTA CTATAAACAC ATTACCTACC ACAGAAGATC TGATGTTGAG ACAATATGTT 
TATGGACCCA GGATACAGAT TTATAAAGAA GACTGTGAGC TTCTTCGACA AAAGATTGAC 
GAATTAAAGA TATCTGCATT GAACCAAAAT AAACTATTGG CGGATGTAAA TAGGAACTTG 
TGGGAAAAAG TGAAAGACTA CTCTGATGAA GAGCTGAAGA ACTATGGGAT TTACCTTAAC 
AAAATCAAAT CACGTTATAC CAAGATGACA AAAGTCTTCA ATCACCAAGG AAAAGTGGCT 
CTGTATAACA AATTGGTGCA TTCAGCTGAG AATGAGAAGA ACAAACTTCA GATAAAAATA 
AATGAGATGG ACACTATCCT CAAGAAGATC AATAACTGTC TCGCTGAAGT GGAAACAGAA 
ACTAAGAATT TGGAGAATGA AGAGAAAAAT GATGCCATGG AAGAATGGGA TTCTGAAATG 
AGAGATGCAG AGAAAGAACT GGAACAGCTG AAAACTGAAG AAGAGGAGCT TCAGAGAAAG 
TTCTTAGAAG TAGAGACACA GAAAACGCAG ACCCTTGCTC AGATAGAATT TATAAAAGAA 
CAAACAACTA AAACTGAAGA ATTACTGGAT CAATTGAGTT TGTCTGAGTG GGATGTCATT 
GAGTGGAGTG ATGATCAAGC TGTATTTACC TTTGTCTATG ATTCAATAGA ACTCATCATC 
ACCTTTGGAG AGCCACTAGT TGGTCTCCCT TTTTTGGACA AGGCTTGCAG GAAGATTAAT 
GCACTCAGTT TTCAGTCTCT GTTAGATGAA GATAAAGCTC CTCCCTCCTC CCTTTTAGTT 
CACAAGCTTA TTTTTCAGTA CATTGAGGAA CAGGAATCCT GGAAGAAGAA ATGCACAGCA 
CAGCATCAGG TGCCCCAGAT GCTTCAAGAA CTCTCACTGG TAGTGAACCA TTGTAGGCTT 
CTTGGAGAGG AGATCGAATT TTTAAAGCGA TGGGGGCCAA ATTATAGCCT AATGCATATA 
AATGTGAATA ATACTGAATT GAGGCTTTTA TTCTCCAGTT GTGCAGCATT TGCAAAGTTT 
GAGATAACTT TGTCTCCCTC GGCCCATTAT CCCTTGGTCC CGTTACCGTT CACCATTCAC 
AATCACATTG GGAAGACTGG CCATGATGAA ATTGCTGCAA TTATCTCTAA AGTGCCCTTA 
GAGGAAAACT ACCTGAAGAA TGTAGTCAAG CAAATTTACC AAGATCTACT TAAGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006500439.1
CDS	540..6899
Translation	MDGVYSEANEENDNTQRPVRRQHSSILKPPRSPLQDLKCGNQTNQEPNPPRKRKSSRRVSFADTIKVFQTESHMKTERNSEISGMNTLLCAPIQTQMQQKEFSITDCNHERKHANDQTVIFSDENQMDLTASHTVMITKGLSDCTKNENSTKIDTTSFLENLKHHAANSRIKKDLACSTVSLSQNIFSEKINSDNFIKRLKTGKHISSSTELDKENAEIPVYSKDSNSASSTYQMHASLGVDENSSNRTRIFREQDDGMNLTQCHTACIKTWIPPSTEAKIGEFKGDKTIYGNECMELTTNYTIQVLSSENNLSERETQTQNGMNVTTVDGATAPAPEKKTALKDKLNAAFQGSFPNPENKIHIIKCHPIESETHTVTQISSQSASTLAVTSKSICSSPAIEGYKTIFHSSSNDAMELTKCLSAMEEEKKLLKADDKYSKICTNPDAGPLREKTIYLEEDSMDITKSHTVAIDNKIFKHDQENIKKEIIAIPIFEKEMVLRNLMPMSKDEKRDVNYISVPQVSKESLQRSQTNTLSVSLTDKKMEFLADEDMDLTKSHTTKLSQVIPTTFDLASKNVTKSYSHSKSPLNEWESLDKQVVLGQHSKLPLPQRKDRDDPDCSHHKIMYSEELQTMDLTKSHTIVIGFGPSEVQEHSKINLEHKNSQLTAESIQTAVNVPAANSRVVTTNDMDMLKDRSTHKPELLKEKQNIKIYGRKSIGRLKIDKTILFSEGNEGDMDITKSCTVKINHRSLLDKHDSHLVSLAGTSKTILHARGQVEMEINRSHTTPLECKIISPSDITPGDLDKTMMSIDDHEELDMTKSHTVFIDYQAEAKGVLPDRLDFQLSKKESLQKPKVTSLAEEIYISKNSESNHLPAKGSQLTILEEGSNSGLGEETNDAQKPGFLNELLSGKTQRRKSLSLKNKSITFPENDKSYREIPQSSAVEINNETRLEDRKGFSFVPLAGTSKPVLSAYGPEDMEISIGQTTASEYKTVPPEEITTIPMDKTVMFVDNFGDLDVTRSHTVFIDCQAKEKVLDEYTNLGIQKTKTLSGSEGDTHIQEITKNPAAQHKHHMTTVIPSSTVVSDQSSMKIKFHKADRDEEVKGKEVEANMLKQTKPESCLLNITDGKNVDFTSSYTADVCRSSDKYSSLPNISSSDNSGGNTMSLCDKNKEKAYNCQVPNEFTYAAILPSTYHMDSKKLSVFPPCPSKEVTQTESAIALLKDEDPVEEPLGEMATFNSKHVSLNLAKDQTEAFVDVSVASQPHLSAQQSPSTQKGQDVARRDEGILAKAGKKALPFLLENVAASTWENESKIPTNVEHFAVTYEKELSISIQTDKCNTNVQSPSNSALTTQVIQTHANAEGALDFLVPSTVSCFSSTKPSLSNLNRKTEEVLDFQTVNLLPPAEQLLEEGSQAHSMSIVQATEIYRLGSRNDRDEESKTFCNEAETTSVPLKTAVKDKTRRCSLGIFLPKLPSKRSCSITGVDDLEQILADAADLTQLETQPVCSKDPGIGSVAAKLNLSPSQFINEENLPVYPGEILSSDSVSLDIEESVLIDTSQRESLPSENKTENCRAQKRTRVEENDVTNEKKIRTHDSAQDQEIFDNHMEEDINKNVNSVLLKSLSRTPSSCSSSLDSIKSDGLSLDVSTQRNSQMESQFLGDTISEESLKEKLKDGQITIKEFFILLQVHILIQKPRQSTLPAKFTINTLPTTEDLMLRQYVYGPRIQIYKEDCELLRQKIDELKISALNQNKLLADVNRNLWEKVKDYSDEELKNYGIYLNKIKSRYTKMTKVFNHQGKVALYNKLVHSAENEKNKLQIKINEMDTILKKINNCLAEVETETKNLENEEKNDAMEEWDSEMRDAEKELEQLKTEEEELQRKFLEVETQKTQTLAQIEFIKEQTTKTEELLDQLSLSEWDVIEWSDDQAVFTFVYDSIELIITFGEPLVGLPFLDKACRKINALSFQSLLDEDKAPPSSLLVHKLIFQYIEEQESWKKKCTAQHQVPQMLQELSLVVNHCRLLGEEIEFLKRWGPNYSLMHINVNNTELRLLFSSCAAFAKFEITLSPSAHYPLVPLPFTIHNHIGKTGHDEIAAIISKVPLEENYLKNVVKQIYQDLLKD

Target ORF information:

RefSeq Version	XM_006500376.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus cancer susceptibility candidate 5 (Casc5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006500376.2

ORF Insert Sequence:

ATGGATGGGG TATATTCAGA AGCTAATGAA GAGAATGACA ATACACAGAG ACCTGTTAGA 
AGACAGCATT CCTCAATTTT GAAACCCCCA AGGAGTCCTC TTCAGGACCT CAAATGTGGA 
AATCAAACAA ACCAGGAACC TAATCCTCCA AGAAAAAGGA AAAGCTCTCG TCGAGTCAGC 
TTTGCAGATA CTATAAAAGT ATTCCAGACA GAGTCTCACA TGAAAACAGA GAGAAATTCA 
GAAATTTCAG GTATGAACAC TTTGCTTTGT GCTCCAATTC AGACTCAGAT GCAACAAAAG 
GAGTTCTCAA TTACAGACTG CAACCATGAA AGGAAACATG CAAATGATCA GACAGTCATC 
TTTTCAGATG AAAACCAGAT GGATCTGACA GCAAGTCACA CTGTAATGAT TACCAAAGGT 
CTTTCAGACT GTACCAAAAA TGAAAATTCT ACAAAAATAG ATACCACGTC CTTTCTGGAG 
AATTTAAAGC ATCATGCTGC AAATTCAAGA ATTAAAAAGG ATTTAGCATG TTCCACAGTT 
TCCTTAAGTC AAAACATTTT CTCAGAAAAG ATCAATTCTG ACAACTTCAT AAAAAGATTG 
AAGACAGGAA AACATATTTC GTCTTCTACA GAGCTTGATA AGGAAAATGC TGAGATACCT 
GTTTATTCCA AAGACTCAAA CAGTGCCTCT TCAACATATC AAATGCATGC ATCTCTTGGT 
GTAGATGAAA ATAGTAGTAA TAGGACTAGA ATCTTTAGAG AACAAGATGA TGGTATGAAT 
TTGACCCAAT GTCATACAGC CTGTATTAAG ACTTGGATTC CTCCATCCAC GGAGGCCAAA 
ATAGGAGAAT TCAAAGGTGA TAAAACAATT TATGGCAATG AGTGTATGGA GTTGACAACT 
AACTATACGA TACAGGTTTT GTCATCAGAA AATAATTTAT CTGAAAGAGA AACTCAAACT 
CAAAATGGTA TGAATGTTAC AACAGTTGAT GGAGCTACAG CTCCAGCTCC AGAGAAGAAA 
ACAGCCCTTA AGGATAAACT AAATGCTGCT TTTCAAGGCT CTTTCCCAAA CCCTGAAAAT 
AAAATTCATA TTATCAAATG TCATCCTATA GAGTCAGAAA CTCATACAGT CACACAGATT 
TCTAGTCAAA GTGCCAGCAC ATTGGCTGTA ACTTCAAAAT CTATATGTTC TAGTCCAGCT 
ATTGAGGGGT ATAAGACAAT TTTTCATTCT AGTAGCAATG ATGCAATGGA ACTGACTAAG 
TGTCTATCAG CTATGGAAGA GGAAAAAAAA TTGCTGAAGG CTGATGATAA GTATTCTAAA 
ATATGTACCA ATCCAGATGC TGGCCCACTC AGAGAGAAAA CTATTTATTT AGAAGAGGAT 
AGCATGGACA TTACCAAAAG TCACACCGTT GCAATAGATA ATAAAATATT TAAACATGAT 
CAAGAAAACA TTAAAAAAGA AATAATAGCC ATACCAATAT TTGAAAAGGA AATGGTGCTC 
CGAAATCTTA TGCCTATGTC AAAAGATGAG AAAAGGGATG TAAATTATAT TTCAGTTCCT 
CAAGTATCTA AGGAAAGCTT ACAACGGAGT CAGACAAACA CTTTATCTGT TTCATTGACT 
GACAAAAAGA TGGAATTCTT GGCAGATGAA GATATGGATC TGACTAAAAG TCACACAACT 
AAGTTAAGTC AAGTTATTCC TACAACTTTT GACTTAGCAT CAAAGAATGT CACCAAATCA 
TACTCTCACA GCAAAAGCCC TTTAAATGAG TGGGAAAGCC TGGATAAACA AGTAGTGCTA 
GGCCAGCATT CTAAACTTCC TTTACCACAG AGGAAAGATA GAGATGATCC TGACTGTTCT 
CATCATAAGA TAATGTATTC AGAGGAGTTA CAAACTATGG ATCTAACCAA GAGCCACACT 
ATTGTAATTG GCTTTGGTCC TTCTGAAGTA CAAGAACATA GTAAAATTAA TTTAGAACAC 
AAAAACAGCC AACTTACAGC AGAAAGCATA CAAACAGCTG TGAACGTTCC TGCAGCAAAC 
TCTAGAGTAG TAACAACTAA TGATATGGAC ATGTTAAAGG ACAGAAGTAC ACATAAGCCA 
GAATTGCTGA AGGAAAAGCA AAACATCAAA ATCTATGGAA GAAAAAGTAT TGGTAGACTG 
AAAATTGATA AAACTATATT ATTTTCAGAG GGTAATGAGG GTGATATGGA TATCACCAAG 
AGTTGCACAG TCAAAATAAA TCATAGATCT TTATTAGATA AGCATGATTC TCATTTAGTA 
TCTTTGGCAG GAACTTCTAA AACCATTTTG CATGCACGTG GTCAGGTTGA AATGGAAATC 
AATAGAAGTC ACACAACTCC TTTAGAATGT AAAATTATCT CGCCCAGTGA TATAACTCCT 
GGTGATCTGG ATAAGACTAT GATGTCTATA GATGATCATG AGGAACTAGA CATGACCAAG 
TCCCATACTG TTTTCATTGA CTACCAAGCA GAGGCAAAAG GTGTACTTCC AGACAGACTT 
GACTTTCAAT TATCCAAAAA GGAAAGCCTA CAGAAGCCGA AAGTGACATC TCTGGCTGAA 
GAGATTTACA TTTCAAAAAA TAGTGAAAGC AACCATCTAC CAGCAAAAGG CAGCCAGCTA 
ACAATATTAG AGGAAGGGTC TAATAGCGGG CTTGGAGAGG AAACTAATGA TGCACAAAAG 
CCTGGATTTC TGAATGAACT TCTGTCAGGC AAAACTCAGA GAAGAAAAAG CCTTAGCCTC 
AAAAACAAGA GTATTACATT TCCAGAGAAT GATAAAAGTT ATAGAGAGAT CCCTCAGAGT 
TCTGCAGTAG AAATAAATAA TGAAACTAGG CTGGAAGACA GAAAGGGCTT CAGTTTTGTG 
CCATTGGCAG GAACTTCCAA ACCAGTTTTG TCTGCATATG GGCCCGAGGA CATGGAGATT 
TCTATAGGTC AAACCACTGC TTCAGAATAT AAAACTGTCC CACCAGAAGA AATAACTACT 
ATACCTATGG ATAAAACTGT AATGTTTGTA GATAATTTCG GTGATCTGGA TGTCACCAGA 
TCCCATACGG TTTTCATCGA TTGTCAAGCC AAAGAAAAGG TACTTGATGA ATATACTAAC 
TTGGGAATAC AGAAAACAAA AACTTTGAGT GGTTCTGAAG GTGATACCCA TATTCAAGAA 
ATCACAAAAA ACCCAGCAGC TCAACACAAA CACCATATGA CAACTGTTAT TCCTTCTAGT 
ACAGTAGTTA GTGATCAAAG TTCAATGAAA ATCAAGTTTC ACAAAGCTGA TAGAGATGAG 
GAGGTCAAAG GAAAGGAAGT GGAAGCCAAT ATGCTGAAAC AAACTAAACC TGAAAGCTGT 
CTGTTAAATA TAACAGATGG AAAAAATGTG GATTTTACAA GCAGTTATAC AGCTGATGTT 
TGTAGATCCA GTGACAAGTA TTCCTCTTTA CCAAATATTT CTTCTTCTGA TAATTCGGGT 
GGTAATACTA TGTCCTTGTG TGATAAAAAT AAGGAGAAAG CTTATAATTG CCAAGTGCCA 
AATGAATTTA CTTATGCAGC TATCTTACCA AGTACATATC ACATGGATTC TAAAAAACTG 
TCTGTCTTTC CACCTTGTCC TTCAAAAGAA GTCACTCAAA CTGAAAGTGC CATAGCGCTG 
CTTAAAGATG AAGATCCGGT AGAAGAGCCT CTGGGGGAGA TGGCTACATT TAACTCAAAG 
CATGTATCTC TTAACCTGGC AAAGGATCAA ACAGAGGCCT TTGTTGATGT TAGTGTTGCT 
TCTCAGCCTC ATCTCTCAGC CCAACAAAGT CCATCAACTC AAAAAGGGCA GGATGTTGCC 
CGTAGAGATG AAGGAATATT AGCTAAAGCT GGGAAGAAGG CTTTACCTTT TCTTCTAGAA 
AATGTCGCTG CATCCACATG GGAAAATGAG TCCAAAATAC CCACTAATGT GGAACACTTT 
GCTGTAACCT ATGAAAAAGA ACTGAGTATA AGTATTCAAA CAGATAAATG TAATACAAAT 
GTACAGAGCC CTAGTAACTC GGCCCTGACA ACACAAGTCA TTCAGACGCA TGCAAATGCT 
GAAGGAGCAC TAGACTTTTT AGTTCCATCT ACTGTTTCAT GTTTTAGTAG TACCAAACCA 
AGTCTGAGTA ATTTGAATAG AAAAACTGAG GAGGTCTTAG ACTTTCAGAC TGTTAATTTA 
TTACCTCCTG CCGAACAGTT ACTTGAAGAA GGAAGTCAGG CACATAGTAT GAGTATAGTA 
CAAGCTACAG AAATATATAG ACTAGGTTCC AGGAATGATA GAGATGAAGA AAGTAAAACT 
TTCTGTAATG AGGCTGAAAC TACATCTGTA CCACTAAAAA CAGCTGTTAA AGACAAAACA 
AGAAGATGTT CATTGGGGAT ATTTTTACCC AAATTACCAA GCAAGAGAAG TTGTAGTATC 
ACTGGTGTTG ATGACCTGGA ACAGATTCTA GCAGATGCAG CTGACTTAAC TCAACTAGAA 
ACTCAGCCAG TCTGTAGTAA AGATCCAGGC ATTGGATCTG TTGCAGCCAA ACTGAATTTG 
AGTCCATCTC AGTTTATAAA TGAGGAAAAT CTCCCTGTAT ATCCTGGTGA GATACTTTCA 
TCAGATTCTG TTAGCCTGGA CATTGAGGAA AGTGTTCTGA TTGACACATC TCAGAGAGAG 
AGTTTACCGT CTGAAAACAA AACCGAAAAC TGCAGGGCCC AAAAAAGAAC CCGGGTTGAA 
GAAAATGATG TTACAAATGA GAAAAAAATC AGAACACATG ACAGTGCACA AGATCAAGAG 
ATTTTTGATA ATCATATGGA AGAGGATATC AATAAAAATG TCAACAGTGT ATTGCTGAAA 
AGCCTGAGCA GGACACCGTC TAGTTGCAGC AGCTCTCTGG ATTCAATCAA GTCTGATGGA 
CTCTCTCTGG ATGTCAGCAC ACAGCGTAAT AGCCAAATGG AATCACAGTT TCTTGGTGAC 
ACTATCTCTG AAGAGAGTTT GAAGGAGAAA CTCAAGGATG GACAAATTAC AATAAAGGAG 
TTCTTCATAC TTCTTCAGGT CCACATCTTA ATACAGAAAC CTAGGCAGAG CACTCTCCCA 
GCCAAATTTA CTATAAACAC ATTACCTACC ACAGAAGATC TGATGTTGAG ACAATATGTT 
TATGGACCCA GGATACAGAT TTATAAAGAA GACTGTGAGC TTCTTCGACA AAAGATTGAC 
GAATTAAAGA TATCTGCATT GAACCAAAAT AAACTATTGG CGGATGTAAA TAGGAACTTG 
TGGGAAAAAG TGAAAGACTA CTCTGATGAA GAGCTGAAGA ACTATGGGAT TTACCTTAAC 
AAAATCAAAT CACGTTATAC CAAGATGACA AAAGTCTTCA ATCACCAAGG AAAAGTGGCT 
CTGTATAACA AATTGGTGCA TTCAGCTGAG AATGAGAAGA ACAAACTTCA GATAAAAATA 
AATGAGATGG ACACTATCCT CAAGAAGATC AATAACTGTC TCGCTGAAGT GGAAACAGAA 
ACTAAGAATT TGGAGAATGA AGAGAAAAAT GATGCCATGG AAGAATGGGA TTCTGAAATG 
AGAGATGCAG AGAAAGAACT GGAACAGCTG AAAACTGAAG AAGAGGAGCT TCAGAGAAAG 
TTCTTAGAAG TAGAGACACA GAAAACGCAG ACCCTTGCTC AGATAGAATT TATAAAAGAA 
CAAACAACTA AAACTGAAGA ATTACTGGAT CAATTGAGTT TGTCTGAGTG GGATGTCATT 
GAGTGGAGTG ATGATCAAGC TGTATTTACC TTTGTCTATG ATTCAATAGA ACTCATCATC 
ACCTTTGGAG AGCCACTAGT TGGTCTCCCT TTTTTGGACA AGGCTTGCAG GAAGATTAAT 
GCACTCAGTT TTCAGTCTCT GTTAGATGAA GATAAAGCTC CTCCCTCCTC CCTTTTAGTT 
CACAAGCTTA TTTTTCAGTA CATTGAGGAA CAGGAATCCT GGAAGAAGAA ATGCACAGCA 
CAGCATCAGG TGCCCCAGAT GCTTCAAGAA CTCTCACTGG TAGTGAACCA TTGTAGGCTT 
CTTGGAGAGG AGATCGAATT TTTAAAGCGA TGGGGGCCAA ATTATAGCCT AATGCATATA 
AATGTGAATA ATACTGAATT GAGGCTTTTA TTCTCCAGTT GTGCAGCATT TGCAAAGTTT 
GAGATAACTT TGTCTCCCTC GGCCCATTAT CCCTTGGTCC CGTTACCGTT CACCATTCAC 
AATCACATTG GGAAGACTGG CCATGATGAA ATTGCTGCAA TTATCTCTAA AGTGCCCTTA 
GAGGAAAACT ACCTGAAGAA TGTAGTCAAG CAAATTTACC AAGATCTACT TAAGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Knl1 ( XM_006500377.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006500440.1 Mus musculus cancer susceptibility candidate 5 (Casc5), transcript variant X2, mRNA.

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CloneID	OMu07220
Clone ID Related Accession (Same CDS sequence)	XM_006500376.2 , XM_006500377.4 , NM_029617.3 , XM_006500377.3 , NM_029617.2 , XM_006500376.3
Accession Version	XM_006500377.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein CASC5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006500377.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGGGG TATATTCAGA AGCTAATGAA GAGAATGACA ATACACAGAG ACCTGTTAGA 
AGACAGCATT CCTCAATTTT GAAACCCCCA AGGAGTCCTC TTCAGGACCT CAAATGTGGA 
AATCAAACAA ACCAGGAACC TAATCCTCCA AGAAAAAGGA AAAGCTCTCG TCGAGTCAGC 
TTTGCAGATA CTATAAAAGT ATTCCAGACA GAGTCTCACA TGAAAACAGA GAGAAATTCA 
GAAATTTCAG GTATGAACAC TTTGCTTTGT GCTCCAATTC AGACTCAGAT GCAACAAAAG 
GAGTTCTCAA TTACAGACTG CAACCATGAA AGGAAACATG CAAATGATCA GACAGTCATC 
TTTTCAGATG AAAACCAGAT GGATCTGACA GCAAGTCACA CTGTAATGAT TACCAAAGGT 
CTTTCAGACT GTACCAAAAA TGAAAATTCT ACAAAAATAG ATACCACGTC CTTTCTGGAG 
AATTTAAAGC ATCATGCTGC AAATTCAAGA ATTAAAAAGG ATTTAGCATG TTCCACAGTT 
TCCTTAAGTC AAAACATTTT CTCAGAAAAG ATCAATTCTG ACAACTTCAT AAAAAGATTG 
AAGACAGGAA AACATATTTC GTCTTCTACA GAGCTTGATA AGGAAAATGC TGAGATACCT 
GTTTATTCCA AAGACTCAAA CAGTGCCTCT TCAACATATC AAATGCATGC ATCTCTTGGT 
GTAGATGAAA ATAGTAGTAA TAGGACTAGA ATCTTTAGAG AACAAGATGA TGGTATGAAT 
TTGACCCAAT GTCATACAGC CTGTATTAAG ACTTGGATTC CTCCATCCAC GGAGGCCAAA 
ATAGGAGAAT TCAAAGGTGA TAAAACAATT TATGGCAATG AGTGTATGGA GTTGACAACT 
AACTATACGA TACAGGTTTT GTCATCAGAA AATAATTTAT CTGAAAGAGA AACTCAAACT 
CAAAATGGTA TGAATGTTAC AACAGTTGAT GGAGCTACAG CTCCAGCTCC AGAGAAGAAA 
ACAGCCCTTA AGGATAAACT AAATGCTGCT TTTCAAGGCT CTTTCCCAAA CCCTGAAAAT 
AAAATTCATA TTATCAAATG TCATCCTATA GAGTCAGAAA CTCATACAGT CACACAGATT 
TCTAGTCAAA GTGCCAGCAC ATTGGCTGTA ACTTCAAAAT CTATATGTTC TAGTCCAGCT 
ATTGAGGGGT ATAAGACAAT TTTTCATTCT AGTAGCAATG ATGCAATGGA ACTGACTAAG 
TGTCTATCAG CTATGGAAGA GGAAAAAAAA TTGCTGAAGG CTGATGATAA GTATTCTAAA 
ATATGTACCA ATCCAGATGC TGGCCCACTC AGAGAGAAAA CTATTTATTT AGAAGAGGAT 
AGCATGGACA TTACCAAAAG TCACACCGTT GCAATAGATA ATAAAATATT TAAACATGAT 
CAAGAAAACA TTAAAAAAGA AATAATAGCC ATACCAATAT TTGAAAAGGA AATGGTGCTC 
CGAAATCTTA TGCCTATGTC AAAAGATGAG AAAAGGGATG TAAATTATAT TTCAGTTCCT 
CAAGTATCTA AGGAAAGCTT ACAACGGAGT CAGACAAACA CTTTATCTGT TTCATTGACT 
GACAAAAAGA TGGAATTCTT GGCAGATGAA GATATGGATC TGACTAAAAG TCACACAACT 
AAGTTAAGTC AAGTTATTCC TACAACTTTT GACTTAGCAT CAAAGAATGT CACCAAATCA 
TACTCTCACA GCAAAAGCCC TTTAAATGAG TGGGAAAGCC TGGATAAACA AGTAGTGCTA 
GGCCAGCATT CTAAACTTCC TTTACCACAG AGGAAAGATA GAGATGATCC TGACTGTTCT 
CATCATAAGA TAATGTATTC AGAGGAGTTA CAAACTATGG ATCTAACCAA GAGCCACACT 
ATTGTAATTG GCTTTGGTCC TTCTGAAGTA CAAGAACATA GTAAAATTAA TTTAGAACAC 
AAAAACAGCC AACTTACAGC AGAAAGCATA CAAACAGCTG TGAACGTTCC TGCAGCAAAC 
TCTAGAGTAG TAACAACTAA TGATATGGAC ATGTTAAAGG ACAGAAGTAC ACATAAGCCA 
GAATTGCTGA AGGAAAAGCA AAACATCAAA ATCTATGGAA GAAAAAGTAT TGGTAGACTG 
AAAATTGATA AAACTATATT ATTTTCAGAG GGTAATGAGG GTGATATGGA TATCACCAAG 
AGTTGCACAG TCAAAATAAA TCATAGATCT TTATTAGATA AGCATGATTC TCATTTAGTA 
TCTTTGGCAG GAACTTCTAA AACCATTTTG CATGCACGTG GTCAGGTTGA AATGGAAATC 
AATAGAAGTC ACACAACTCC TTTAGAATGT AAAATTATCT CGCCCAGTGA TATAACTCCT 
GGTGATCTGG ATAAGACTAT GATGTCTATA GATGATCATG AGGAACTAGA CATGACCAAG 
TCCCATACTG TTTTCATTGA CTACCAAGCA GAGGCAAAAG GTGTACTTCC AGACAGACTT 
GACTTTCAAT TATCCAAAAA GGAAAGCCTA CAGAAGCCGA AAGTGACATC TCTGGCTGAA 
GAGATTTACA TTTCAAAAAA TAGTGAAAGC AACCATCTAC CAGCAAAAGG CAGCCAGCTA 
ACAATATTAG AGGAAGGGTC TAATAGCGGG CTTGGAGAGG AAACTAATGA TGCACAAAAG 
CCTGGATTTC TGAATGAACT TCTGTCAGGC AAAACTCAGA GAAGAAAAAG CCTTAGCCTC 
AAAAACAAGA GTATTACATT TCCAGAGAAT GATAAAAGTT ATAGAGAGAT CCCTCAGAGT 
TCTGCAGTAG AAATAAATAA TGAAACTAGG CTGGAAGACA GAAAGGGCTT CAGTTTTGTG 
CCATTGGCAG GAACTTCCAA ACCAGTTTTG TCTGCATATG GGCCCGAGGA CATGGAGATT 
TCTATAGGTC AAACCACTGC TTCAGAATAT AAAACTGTCC CACCAGAAGA AATAACTACT 
ATACCTATGG ATAAAACTGT AATGTTTGTA GATAATTTCG GTGATCTGGA TGTCACCAGA 
TCCCATACGG TTTTCATCGA TTGTCAAGCC AAAGAAAAGG TACTTGATGA ATATACTAAC 
TTGGGAATAC AGAAAACAAA AACTTTGAGT GGTTCTGAAG GTGATACCCA TATTCAAGAA 
ATCACAAAAA ACCCAGCAGC TCAACACAAA CACCATATGA CAACTGTTAT TCCTTCTAGT 
ACAGTAGTTA GTGATCAAAG TTCAATGAAA ATCAAGTTTC ACAAAGCTGA TAGAGATGAG 
GAGGTCAAAG GAAAGGAAGT GGAAGCCAAT ATGCTGAAAC AAACTAAACC TGAAAGCTGT 
CTGTTAAATA TAACAGATGG AAAAAATGTG GATTTTACAA GCAGTTATAC AGCTGATGTT 
TGTAGATCCA GTGACAAGTA TTCCTCTTTA CCAAATATTT CTTCTTCTGA TAATTCGGGT 
GGTAATACTA TGTCCTTGTG TGATAAAAAT AAGGAGAAAG CTTATAATTG CCAAGTGCCA 
AATGAATTTA CTTATGCAGC TATCTTACCA AGTACATATC ACATGGATTC TAAAAAACTG 
TCTGTCTTTC CACCTTGTCC TTCAAAAGAA GTCACTCAAA CTGAAAGTGC CATAGCGCTG 
CTTAAAGATG AAGATCCGGT AGAAGAGCCT CTGGGGGAGA TGGCTACATT TAACTCAAAG 
CATGTATCTC TTAACCTGGC AAAGGATCAA ACAGAGGCCT TTGTTGATGT TAGTGTTGCT 
TCTCAGCCTC ATCTCTCAGC CCAACAAAGT CCATCAACTC AAAAAGGGCA GGATGTTGCC 
CGTAGAGATG AAGGAATATT AGCTAAAGCT GGGAAGAAGG CTTTACCTTT TCTTCTAGAA 
AATGTCGCTG CATCCACATG GGAAAATGAG TCCAAAATAC CCACTAATGT GGAACACTTT 
GCTGTAACCT ATGAAAAAGA ACTGAGTATA AGTATTCAAA CAGATAAATG TAATACAAAT 
GTACAGAGCC CTAGTAACTC GGCCCTGACA ACACAAGTCA TTCAGACGCA TGCAAATGCT 
GAAGGAGCAC TAGACTTTTT AGTTCCATCT ACTGTTTCAT GTTTTAGTAG TACCAAACCA 
AGTCTGAGTA ATTTGAATAG AAAAACTGAG GAGGTCTTAG ACTTTCAGAC TGTTAATTTA 
TTACCTCCTG CCGAACAGTT ACTTGAAGAA GGAAGTCAGG CACATAGTAT GAGTATAGTA 
CAAGCTACAG AAATATATAG ACTAGGTTCC AGGAATGATA GAGATGAAGA AAGTAAAACT 
TTCTGTAATG AGGCTGAAAC TACATCTGTA CCACTAAAAA CAGCTGTTAA AGACAAAACA 
AGAAGATGTT CATTGGGGAT ATTTTTACCC AAATTACCAA GCAAGAGAAG TTGTAGTATC 
ACTGGTGTTG ATGACCTGGA ACAGATTCTA GCAGATGCAG CTGACTTAAC TCAACTAGAA 
ACTCAGCCAG TCTGTAGTAA AGATCCAGGC ATTGGATCTG TTGCAGCCAA ACTGAATTTG 
AGTCCATCTC AGTTTATAAA TGAGGAAAAT CTCCCTGTAT ATCCTGGTGA GATACTTTCA 
TCAGATTCTG TTAGCCTGGA CATTGAGGAA AGTGTTCTGA TTGACACATC TCAGAGAGAG 
AGTTTACCGT CTGAAAACAA AACCGAAAAC TGCAGGGCCC AAAAAAGAAC CCGGGTTGAA 
GAAAATGATG TTACAAATGA GAAAAAAATC AGAACACATG ACAGTGCACA AGATCAAGAG 
ATTTTTGATA ATCATATGGA AGAGGATATC AATAAAAATG TCAACAGTGT ATTGCTGAAA 
AGCCTGAGCA GGACACCGTC TAGTTGCAGC AGCTCTCTGG ATTCAATCAA GTCTGATGGA 
CTCTCTCTGG ATGTCAGCAC ACAGCGTAAT AGCCAAATGG AATCACAGTT TCTTGGTGAC 
ACTATCTCTG AAGAGAGTTT GAAGGAGAAA CTCAAGGATG GACAAATTAC AATAAAGGAG 
TTCTTCATAC TTCTTCAGGT CCACATCTTA ATACAGAAAC CTAGGCAGAG CACTCTCCCA 
GCCAAATTTA CTATAAACAC ATTACCTACC ACAGAAGATC TGATGTTGAG ACAATATGTT 
TATGGACCCA GGATACAGAT TTATAAAGAA GACTGTGAGC TTCTTCGACA AAAGATTGAC 
GAATTAAAGA TATCTGCATT GAACCAAAAT AAACTATTGG CGGATGTAAA TAGGAACTTG 
TGGGAAAAAG TGAAAGACTA CTCTGATGAA GAGCTGAAGA ACTATGGGAT TTACCTTAAC 
AAAATCAAAT CACGTTATAC CAAGATGACA AAAGTCTTCA ATCACCAAGG AAAAGTGGCT 
CTGTATAACA AATTGGTGCA TTCAGCTGAG AATGAGAAGA ACAAACTTCA GATAAAAATA 
AATGAGATGG ACACTATCCT CAAGAAGATC AATAACTGTC TCGCTGAAGT GGAAACAGAA 
ACTAAGAATT TGGAGAATGA AGAGAAAAAT GATGCCATGG AAGAATGGGA TTCTGAAATG 
AGAGATGCAG AGAAAGAACT GGAACAGCTG AAAACTGAAG AAGAGGAGCT TCAGAGAAAG 
TTCTTAGAAG TAGAGACACA GAAAACGCAG ACCCTTGCTC AGATAGAATT TATAAAAGAA 
CAAACAACTA AAACTGAAGA ATTACTGGAT CAATTGAGTT TGTCTGAGTG GGATGTCATT 
GAGTGGAGTG ATGATCAAGC TGTATTTACC TTTGTCTATG ATTCAATAGA ACTCATCATC 
ACCTTTGGAG AGCCACTAGT TGGTCTCCCT TTTTTGGACA AGGCTTGCAG GAAGATTAAT 
GCACTCAGTT TTCAGTCTCT GTTAGATGAA GATAAAGCTC CTCCCTCCTC CCTTTTAGTT 
CACAAGCTTA TTTTTCAGTA CATTGAGGAA CAGGAATCCT GGAAGAAGAA ATGCACAGCA 
CAGCATCAGG TGCCCCAGAT GCTTCAAGAA CTCTCACTGG TAGTGAACCA TTGTAGGCTT 
CTTGGAGAGG AGATCGAATT TTTAAAGCGA TGGGGGCCAA ATTATAGCCT AATGCATATA 
AATGTGAATA ATACTGAATT GAGGCTTTTA TTCTCCAGTT GTGCAGCATT TGCAAAGTTT 
GAGATAACTT TGTCTCCCTC GGCCCATTAT CCCTTGGTCC CGTTACCGTT CACCATTCAC 
AATCACATTG GGAAGACTGG CCATGATGAA ATTGCTGCAA TTATCTCTAA AGTGCCCTTA 
GAGGAAAACT ACCTGAAGAA TGTAGTCAAG CAAATTTACC AAGATCTACT TAAGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006500440.1
CDS	144..6503
Translation	MDGVYSEANEENDNTQRPVRRQHSSILKPPRSPLQDLKCGNQTNQEPNPPRKRKSSRRVSFADTIKVFQTESHMKTERNSEISGMNTLLCAPIQTQMQQKEFSITDCNHERKHANDQTVIFSDENQMDLTASHTVMITKGLSDCTKNENSTKIDTTSFLENLKHHAANSRIKKDLACSTVSLSQNIFSEKINSDNFIKRLKTGKHISSSTELDKENAEIPVYSKDSNSASSTYQMHASLGVDENSSNRTRIFREQDDGMNLTQCHTACIKTWIPPSTEAKIGEFKGDKTIYGNECMELTTNYTIQVLSSENNLSERETQTQNGMNVTTVDGATAPAPEKKTALKDKLNAAFQGSFPNPENKIHIIKCHPIESETHTVTQISSQSASTLAVTSKSICSSPAIEGYKTIFHSSSNDAMELTKCLSAMEEEKKLLKADDKYSKICTNPDAGPLREKTIYLEEDSMDITKSHTVAIDNKIFKHDQENIKKEIIAIPIFEKEMVLRNLMPMSKDEKRDVNYISVPQVSKESLQRSQTNTLSVSLTDKKMEFLADEDMDLTKSHTTKLSQVIPTTFDLASKNVTKSYSHSKSPLNEWESLDKQVVLGQHSKLPLPQRKDRDDPDCSHHKIMYSEELQTMDLTKSHTIVIGFGPSEVQEHSKINLEHKNSQLTAESIQTAVNVPAANSRVVTTNDMDMLKDRSTHKPELLKEKQNIKIYGRKSIGRLKIDKTILFSEGNEGDMDITKSCTVKINHRSLLDKHDSHLVSLAGTSKTILHARGQVEMEINRSHTTPLECKIISPSDITPGDLDKTMMSIDDHEELDMTKSHTVFIDYQAEAKGVLPDRLDFQLSKKESLQKPKVTSLAEEIYISKNSESNHLPAKGSQLTILEEGSNSGLGEETNDAQKPGFLNELLSGKTQRRKSLSLKNKSITFPENDKSYREIPQSSAVEINNETRLEDRKGFSFVPLAGTSKPVLSAYGPEDMEISIGQTTASEYKTVPPEEITTIPMDKTVMFVDNFGDLDVTRSHTVFIDCQAKEKVLDEYTNLGIQKTKTLSGSEGDTHIQEITKNPAAQHKHHMTTVIPSSTVVSDQSSMKIKFHKADRDEEVKGKEVEANMLKQTKPESCLLNITDGKNVDFTSSYTADVCRSSDKYSSLPNISSSDNSGGNTMSLCDKNKEKAYNCQVPNEFTYAAILPSTYHMDSKKLSVFPPCPSKEVTQTESAIALLKDEDPVEEPLGEMATFNSKHVSLNLAKDQTEAFVDVSVASQPHLSAQQSPSTQKGQDVARRDEGILAKAGKKALPFLLENVAASTWENESKIPTNVEHFAVTYEKELSISIQTDKCNTNVQSPSNSALTTQVIQTHANAEGALDFLVPSTVSCFSSTKPSLSNLNRKTEEVLDFQTVNLLPPAEQLLEEGSQAHSMSIVQATEIYRLGSRNDRDEESKTFCNEAETTSVPLKTAVKDKTRRCSLGIFLPKLPSKRSCSITGVDDLEQILADAADLTQLETQPVCSKDPGIGSVAAKLNLSPSQFINEENLPVYPGEILSSDSVSLDIEESVLIDTSQRESLPSENKTENCRAQKRTRVEENDVTNEKKIRTHDSAQDQEIFDNHMEEDINKNVNSVLLKSLSRTPSSCSSSLDSIKSDGLSLDVSTQRNSQMESQFLGDTISEESLKEKLKDGQITIKEFFILLQVHILIQKPRQSTLPAKFTINTLPTTEDLMLRQYVYGPRIQIYKEDCELLRQKIDELKISALNQNKLLADVNRNLWEKVKDYSDEELKNYGIYLNKIKSRYTKMTKVFNHQGKVALYNKLVHSAENEKNKLQIKINEMDTILKKINNCLAEVETETKNLENEEKNDAMEEWDSEMRDAEKELEQLKTEEEELQRKFLEVETQKTQTLAQIEFIKEQTTKTEELLDQLSLSEWDVIEWSDDQAVFTFVYDSIELIITFGEPLVGLPFLDKACRKINALSFQSLLDEDKAPPSSLLVHKLIFQYIEEQESWKKKCTAQHQVPQMLQELSLVVNHCRLLGEEIEFLKRWGPNYSLMHINVNNTELRLLFSSCAAFAKFEITLSPSAHYPLVPLPFTIHNHIGKTGHDEIAAIISKVPLEENYLKNVVKQIYQDLLKD

Target ORF information:

RefSeq Version	XM_006500377.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus cancer susceptibility candidate 5 (Casc5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006500377.3

ORF Insert Sequence:

ATGGATGGGG TATATTCAGA AGCTAATGAA GAGAATGACA ATACACAGAG ACCTGTTAGA 
AGACAGCATT CCTCAATTTT GAAACCCCCA AGGAGTCCTC TTCAGGACCT CAAATGTGGA 
AATCAAACAA ACCAGGAACC TAATCCTCCA AGAAAAAGGA AAAGCTCTCG TCGAGTCAGC 
TTTGCAGATA CTATAAAAGT ATTCCAGACA GAGTCTCACA TGAAAACAGA GAGAAATTCA 
GAAATTTCAG GTATGAACAC TTTGCTTTGT GCTCCAATTC AGACTCAGAT GCAACAAAAG 
GAGTTCTCAA TTACAGACTG CAACCATGAA AGGAAACATG CAAATGATCA GACAGTCATC 
TTTTCAGATG AAAACCAGAT GGATCTGACA GCAAGTCACA CTGTAATGAT TACCAAAGGT 
CTTTCAGACT GTACCAAAAA TGAAAATTCT ACAAAAATAG ATACCACGTC CTTTCTGGAG 
AATTTAAAGC ATCATGCTGC AAATTCAAGA ATTAAAAAGG ATTTAGCATG TTCCACAGTT 
TCCTTAAGTC AAAACATTTT CTCAGAAAAG ATCAATTCTG ACAACTTCAT AAAAAGATTG 
AAGACAGGAA AACATATTTC GTCTTCTACA GAGCTTGATA AGGAAAATGC TGAGATACCT 
GTTTATTCCA AAGACTCAAA CAGTGCCTCT TCAACATATC AAATGCATGC ATCTCTTGGT 
GTAGATGAAA ATAGTAGTAA TAGGACTAGA ATCTTTAGAG AACAAGATGA TGGTATGAAT 
TTGACCCAAT GTCATACAGC CTGTATTAAG ACTTGGATTC CTCCATCCAC GGAGGCCAAA 
ATAGGAGAAT TCAAAGGTGA TAAAACAATT TATGGCAATG AGTGTATGGA GTTGACAACT 
AACTATACGA TACAGGTTTT GTCATCAGAA AATAATTTAT CTGAAAGAGA AACTCAAACT 
CAAAATGGTA TGAATGTTAC AACAGTTGAT GGAGCTACAG CTCCAGCTCC AGAGAAGAAA 
ACAGCCCTTA AGGATAAACT AAATGCTGCT TTTCAAGGCT CTTTCCCAAA CCCTGAAAAT 
AAAATTCATA TTATCAAATG TCATCCTATA GAGTCAGAAA CTCATACAGT CACACAGATT 
TCTAGTCAAA GTGCCAGCAC ATTGGCTGTA ACTTCAAAAT CTATATGTTC TAGTCCAGCT 
ATTGAGGGGT ATAAGACAAT TTTTCATTCT AGTAGCAATG ATGCAATGGA ACTGACTAAG 
TGTCTATCAG CTATGGAAGA GGAAAAAAAA TTGCTGAAGG CTGATGATAA GTATTCTAAA 
ATATGTACCA ATCCAGATGC TGGCCCACTC AGAGAGAAAA CTATTTATTT AGAAGAGGAT 
AGCATGGACA TTACCAAAAG TCACACCGTT GCAATAGATA ATAAAATATT TAAACATGAT 
CAAGAAAACA TTAAAAAAGA AATAATAGCC ATACCAATAT TTGAAAAGGA AATGGTGCTC 
CGAAATCTTA TGCCTATGTC AAAAGATGAG AAAAGGGATG TAAATTATAT TTCAGTTCCT 
CAAGTATCTA AGGAAAGCTT ACAACGGAGT CAGACAAACA CTTTATCTGT TTCATTGACT 
GACAAAAAGA TGGAATTCTT GGCAGATGAA GATATGGATC TGACTAAAAG TCACACAACT 
AAGTTAAGTC AAGTTATTCC TACAACTTTT GACTTAGCAT CAAAGAATGT CACCAAATCA 
TACTCTCACA GCAAAAGCCC TTTAAATGAG TGGGAAAGCC TGGATAAACA AGTAGTGCTA 
GGCCAGCATT CTAAACTTCC TTTACCACAG AGGAAAGATA GAGATGATCC TGACTGTTCT 
CATCATAAGA TAATGTATTC AGAGGAGTTA CAAACTATGG ATCTAACCAA GAGCCACACT 
ATTGTAATTG GCTTTGGTCC TTCTGAAGTA CAAGAACATA GTAAAATTAA TTTAGAACAC 
AAAAACAGCC AACTTACAGC AGAAAGCATA CAAACAGCTG TGAACGTTCC TGCAGCAAAC 
TCTAGAGTAG TAACAACTAA TGATATGGAC ATGTTAAAGG ACAGAAGTAC ACATAAGCCA 
GAATTGCTGA AGGAAAAGCA AAACATCAAA ATCTATGGAA GAAAAAGTAT TGGTAGACTG 
AAAATTGATA AAACTATATT ATTTTCAGAG GGTAATGAGG GTGATATGGA TATCACCAAG 
AGTTGCACAG TCAAAATAAA TCATAGATCT TTATTAGATA AGCATGATTC TCATTTAGTA 
TCTTTGGCAG GAACTTCTAA AACCATTTTG CATGCACGTG GTCAGGTTGA AATGGAAATC 
AATAGAAGTC ACACAACTCC TTTAGAATGT AAAATTATCT CGCCCAGTGA TATAACTCCT 
GGTGATCTGG ATAAGACTAT GATGTCTATA GATGATCATG AGGAACTAGA CATGACCAAG 
TCCCATACTG TTTTCATTGA CTACCAAGCA GAGGCAAAAG GTGTACTTCC AGACAGACTT 
GACTTTCAAT TATCCAAAAA GGAAAGCCTA CAGAAGCCGA AAGTGACATC TCTGGCTGAA 
GAGATTTACA TTTCAAAAAA TAGTGAAAGC AACCATCTAC CAGCAAAAGG CAGCCAGCTA 
ACAATATTAG AGGAAGGGTC TAATAGCGGG CTTGGAGAGG AAACTAATGA TGCACAAAAG 
CCTGGATTTC TGAATGAACT TCTGTCAGGC AAAACTCAGA GAAGAAAAAG CCTTAGCCTC 
AAAAACAAGA GTATTACATT TCCAGAGAAT GATAAAAGTT ATAGAGAGAT CCCTCAGAGT 
TCTGCAGTAG AAATAAATAA TGAAACTAGG CTGGAAGACA GAAAGGGCTT CAGTTTTGTG 
CCATTGGCAG GAACTTCCAA ACCAGTTTTG TCTGCATATG GGCCCGAGGA CATGGAGATT 
TCTATAGGTC AAACCACTGC TTCAGAATAT AAAACTGTCC CACCAGAAGA AATAACTACT 
ATACCTATGG ATAAAACTGT AATGTTTGTA GATAATTTCG GTGATCTGGA TGTCACCAGA 
TCCCATACGG TTTTCATCGA TTGTCAAGCC AAAGAAAAGG TACTTGATGA ATATACTAAC 
TTGGGAATAC AGAAAACAAA AACTTTGAGT GGTTCTGAAG GTGATACCCA TATTCAAGAA 
ATCACAAAAA ACCCAGCAGC TCAACACAAA CACCATATGA CAACTGTTAT TCCTTCTAGT 
ACAGTAGTTA GTGATCAAAG TTCAATGAAA ATCAAGTTTC ACAAAGCTGA TAGAGATGAG 
GAGGTCAAAG GAAAGGAAGT GGAAGCCAAT ATGCTGAAAC AAACTAAACC TGAAAGCTGT 
CTGTTAAATA TAACAGATGG AAAAAATGTG GATTTTACAA GCAGTTATAC AGCTGATGTT 
TGTAGATCCA GTGACAAGTA TTCCTCTTTA CCAAATATTT CTTCTTCTGA TAATTCGGGT 
GGTAATACTA TGTCCTTGTG TGATAAAAAT AAGGAGAAAG CTTATAATTG CCAAGTGCCA 
AATGAATTTA CTTATGCAGC TATCTTACCA AGTACATATC ACATGGATTC TAAAAAACTG 
TCTGTCTTTC CACCTTGTCC TTCAAAAGAA GTCACTCAAA CTGAAAGTGC CATAGCGCTG 
CTTAAAGATG AAGATCCGGT AGAAGAGCCT CTGGGGGAGA TGGCTACATT TAACTCAAAG 
CATGTATCTC TTAACCTGGC AAAGGATCAA ACAGAGGCCT TTGTTGATGT TAGTGTTGCT 
TCTCAGCCTC ATCTCTCAGC CCAACAAAGT CCATCAACTC AAAAAGGGCA GGATGTTGCC 
CGTAGAGATG AAGGAATATT AGCTAAAGCT GGGAAGAAGG CTTTACCTTT TCTTCTAGAA 
AATGTCGCTG CATCCACATG GGAAAATGAG TCCAAAATAC CCACTAATGT GGAACACTTT 
GCTGTAACCT ATGAAAAAGA ACTGAGTATA AGTATTCAAA CAGATAAATG TAATACAAAT 
GTACAGAGCC CTAGTAACTC GGCCCTGACA ACACAAGTCA TTCAGACGCA TGCAAATGCT 
GAAGGAGCAC TAGACTTTTT AGTTCCATCT ACTGTTTCAT GTTTTAGTAG TACCAAACCA 
AGTCTGAGTA ATTTGAATAG AAAAACTGAG GAGGTCTTAG ACTTTCAGAC TGTTAATTTA 
TTACCTCCTG CCGAACAGTT ACTTGAAGAA GGAAGTCAGG CACATAGTAT GAGTATAGTA 
CAAGCTACAG AAATATATAG ACTAGGTTCC AGGAATGATA GAGATGAAGA AAGTAAAACT 
TTCTGTAATG AGGCTGAAAC TACATCTGTA CCACTAAAAA CAGCTGTTAA AGACAAAACA 
AGAAGATGTT CATTGGGGAT ATTTTTACCC AAATTACCAA GCAAGAGAAG TTGTAGTATC 
ACTGGTGTTG ATGACCTGGA ACAGATTCTA GCAGATGCAG CTGACTTAAC TCAACTAGAA 
ACTCAGCCAG TCTGTAGTAA AGATCCAGGC ATTGGATCTG TTGCAGCCAA ACTGAATTTG 
AGTCCATCTC AGTTTATAAA TGAGGAAAAT CTCCCTGTAT ATCCTGGTGA GATACTTTCA 
TCAGATTCTG TTAGCCTGGA CATTGAGGAA AGTGTTCTGA TTGACACATC TCAGAGAGAG 
AGTTTACCGT CTGAAAACAA AACCGAAAAC TGCAGGGCCC AAAAAAGAAC CCGGGTTGAA 
GAAAATGATG TTACAAATGA GAAAAAAATC AGAACACATG ACAGTGCACA AGATCAAGAG 
ATTTTTGATA ATCATATGGA AGAGGATATC AATAAAAATG TCAACAGTGT ATTGCTGAAA 
AGCCTGAGCA GGACACCGTC TAGTTGCAGC AGCTCTCTGG ATTCAATCAA GTCTGATGGA 
CTCTCTCTGG ATGTCAGCAC ACAGCGTAAT AGCCAAATGG AATCACAGTT TCTTGGTGAC 
ACTATCTCTG AAGAGAGTTT GAAGGAGAAA CTCAAGGATG GACAAATTAC AATAAAGGAG 
TTCTTCATAC TTCTTCAGGT CCACATCTTA ATACAGAAAC CTAGGCAGAG CACTCTCCCA 
GCCAAATTTA CTATAAACAC ATTACCTACC ACAGAAGATC TGATGTTGAG ACAATATGTT 
TATGGACCCA GGATACAGAT TTATAAAGAA GACTGTGAGC TTCTTCGACA AAAGATTGAC 
GAATTAAAGA TATCTGCATT GAACCAAAAT AAACTATTGG CGGATGTAAA TAGGAACTTG 
TGGGAAAAAG TGAAAGACTA CTCTGATGAA GAGCTGAAGA ACTATGGGAT TTACCTTAAC 
AAAATCAAAT CACGTTATAC CAAGATGACA AAAGTCTTCA ATCACCAAGG AAAAGTGGCT 
CTGTATAACA AATTGGTGCA TTCAGCTGAG AATGAGAAGA ACAAACTTCA GATAAAAATA 
AATGAGATGG ACACTATCCT CAAGAAGATC AATAACTGTC TCGCTGAAGT GGAAACAGAA 
ACTAAGAATT TGGAGAATGA AGAGAAAAAT GATGCCATGG AAGAATGGGA TTCTGAAATG 
AGAGATGCAG AGAAAGAACT GGAACAGCTG AAAACTGAAG AAGAGGAGCT TCAGAGAAAG 
TTCTTAGAAG TAGAGACACA GAAAACGCAG ACCCTTGCTC AGATAGAATT TATAAAAGAA 
CAAACAACTA AAACTGAAGA ATTACTGGAT CAATTGAGTT TGTCTGAGTG GGATGTCATT 
GAGTGGAGTG ATGATCAAGC TGTATTTACC TTTGTCTATG ATTCAATAGA ACTCATCATC 
ACCTTTGGAG AGCCACTAGT TGGTCTCCCT TTTTTGGACA AGGCTTGCAG GAAGATTAAT 
GCACTCAGTT TTCAGTCTCT GTTAGATGAA GATAAAGCTC CTCCCTCCTC CCTTTTAGTT 
CACAAGCTTA TTTTTCAGTA CATTGAGGAA CAGGAATCCT GGAAGAAGAA ATGCACAGCA 
CAGCATCAGG TGCCCCAGAT GCTTCAAGAA CTCTCACTGG TAGTGAACCA TTGTAGGCTT 
CTTGGAGAGG AGATCGAATT TTTAAAGCGA TGGGGGCCAA ATTATAGCCT AATGCATATA 
AATGTGAATA ATACTGAATT GAGGCTTTTA TTCTCCAGTT GTGCAGCATT TGCAAAGTTT 
GAGATAACTT TGTCTCCCTC GGCCCATTAT CCCTTGGTCC CGTTACCGTT CACCATTCAC 
AATCACATTG GGAAGACTGG CCATGATGAA ATTGCTGCAA TTATCTCTAA AGTGCCCTTA 
GAGGAAAACT ACCTGAAGAA TGTAGTCAAG CAAATTTACC AAGATCTACT TAAGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structural analysis reveals features of the spindle checkpoint kinase Bub1-kinetochore subunit Knl1 interaction.
The Journal of cell biology196(4)451-67(2012 Feb)
Krenn V,Wehenkel A,Li X,Santaguida S,Musacchio A

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Knl1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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