KNL1 cDNA ORF clone, Gallus gallus(chicken)

The following KNL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KNL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa151173 XM_025151153.1
Latest version!
Gallus gallus kinetochore scaffold 1 (KNL1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OGa36086 XM_015286271.1
Latest version!
Gallus gallus kinetochore scaffold 1 (KNL1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OGa08803 XM_420938.5
Latest version!
Gallus gallus kinetochore scaffold 1 (KNL1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OGa08804 XM_004941298.2
Latest version!
Gallus gallus kinetochore scaffold 1 (KNL1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa151173
    Clone ID Related Accession (Same CDS sequence) XM_025151153.1
    Accession Version XM_025151153.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6816bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product kinetochore scaffold 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGGGTGCTG TGGGGCTGGG GGTCAGCGGC GGAGAGCGGC CGAGAGTGTG CCATGATGTG 
    GACAATACTA TTCCGAGAAC AGATAGGCCT GATGCAACAG TCATCTTCTC AGATGAAAAT
    GAAATGGATA TGACAGCTAG TCACACTGCA GTGATTGCAC GTAACCTGAA TAACAGTGAA
    ACTGACAAAA CTGAGAAAAT AGATATTGCG TCATTTCTGG CTGAGTTAAA TTCTATCAAT
    GGAAAAGCAG AAAAAAACAA AGATTTTCAT TTTTCCTTTG ATGCTGCAGA CCGTTTTTGC
    CCATCTTTTG AACAGAAGGA AGATGCTACT ACAGGAAAGA AAATAAATTC CAATGAATTT
    TTCATGAACT CAAATGAAAA AGCACTTAAT CCTATTGAAG GACCTGAAAA AGAGAACCTT
    TTTTTTGTCC CTTCAGAAGT GTCAGAAAAC ATGGCACGAT CATCAGTGGA ATATGTATAT
    TCCCATGAAC AACTAGCCAC CTGCAATGTA ACAAAAATAT TTAGAGAGCA AGATGATGGA
    ATGGAAATAA CAAAGTGCCA AGCATCTGAT ATTCAAAATA TGCATTCTGG TCTTTCTGAG
    GTTCCGACAG AGCAGTTAGC ATCTGCAGAT GTTACTCAGG CATTTGCAGA TGATAGAATG
    GATATGACAA CTAATCACAC TGCAAAAATA CATTTTCCCT TTGCCATTGT AGGTAATCAA
    AGTTTAAATT CCAGAAAGGA TTTCTCATCC ATGGAGCTAG ATAACTACTG TATTGTAAAG
    AGGACATCTC ACCAGCAATT AATCCTAAAG CAAGACCCTC AACTTTGTAC ACGCAAAAAA
    ATCATAAATG TTGAAGACAC AGTAGATGCT ACAGCACTGC AGGCTTTTAA GCAGGAGAGC
    AGAACATTGT CTTCTATCCC AGGATCTGTT TCTTCTGAGA CTGTCTTCCG AGATGATAAA
    ACTGTTGTTT TTTCTCAATG TGATGACATG GAAATTACTG GGAATTACAG AGATGTACTC
    TATAATACAA GTACCAGAGG AATGAACAGT TCATGTAATA AAGCTTTTGA AAAAACAGTT
    AACAGAAGTT CATTGCTAGT AGAAAGACGA CATCCGGTAA ATAATGACAA TACAAGAAGT
    CTGTCTTTAG ATGATAGATC TTCTCCGTCA CGCAAGAATA GTGCACGTGA ACTATCTTCA
    GGTTCAGATG GACAAAATGA AAGAGTAACC CAAGATCACA GTACTGTTTC ATTGAACACT
    GGTTTCAATT CATGTACAAA TTCAATGTCT ACTGGTTCCT CTAAGGGTAG GCTGCAGCAC
    AGACTAGCAA ACTCCCTACT TGTTTCACTA CCAGGTGAGA AAACTGCAAT ATTTTCAGGA
    ACAGATGTGG ACTTGACTAA AAATTGTGTT GGCAAGGGTT ATAGAAAGAA TATTGAAAAT
    GAATTTCCTC CTGGATTGTC CAACTCTGTC ACTTGCAAAC CACATTTTCT TGATAGTAGG
    TCTGTATCTT CCAATTTAAG GGAGCAGGAA AAAATGGAAA TAACTAAATG TCAGACAGTT
    GTCAGTGGTG ATCAAAGCAT TGGGATAACC ACAGAAGCAG AACAAAGGCA TTGTAAAATA
    ATTCCAAGTA AGAACCAGAA CAATAATGTT AGTGATTCTT TGGATAGGGA CAAGGAAAAT
    CTTGAGGCAA TTGGTTTTGA TGTGAGTATT AGAAGAAGCC AGGCTATAGA AGCGAATGCT
    AAAGGTGTTG AGGTGGTAAC TGTCAATAAG AAGCCTGGAA AAACAGACTT TCAGGCTAGT
    AGTCTGAGTT CCTGTGCAAA GAGCAGGTAT TCACTCCAGG AGCAAAAGAG GGAAGTCTCT
    GAAAATATTG TCACTGACAA ATGTGCATTC ACATCTTTGC AAAGTGAAAA AGTATATGTA
    TCCCAGCCTT TGGGAAATAA ACTTCCAAAT TCTTCAGTTT CCTGCACAAA TGACAAAAGA
    GACATGTTTT CAGATGATGA AAACATGGAC ATAACCAAAG CTCATTCTTT GGTTGCTCCT
    TCTATGAATA TAGTACAAGA AAATGAGAAT ACTCACGTTC AAAATAATGT AAAAGCCAAA
    CAGCTGCAAA ACCAGACCTT CCAGTTTTCT GATAATTCTG GTATAGATCA TACAAGTCAG
    ACTGCAGCAA TAGAATGTGG AGATGTCAAA AGGAGCTCAA ATAAACAAAC TGTTGCTCAT
    TTAGCTCCAA GTGGTTTCTT TATTTCCAGT AATGAGCTTG CTCCCTCTGG AAAGAAAAGA
    GATGCACAAC CTGGAAATAC ACTGGGAATA ATCTCAGATT GCCAGAACTC TGATAGGCAT
    AAGAAAACAC ATCCTAGGAT AGTAGTAAAC AATGTATCAT CAACTGCAGA AGACCGTGAG
    AGTGATTTTT TGATCAGTAA AACAGTTTCT TTAGAAGGGG GTTTTAATAA CCCTCAGTCA
    GGTGATGACC TGTGCTTGAG GGGTGTTGAA GAGAGGTCAC TAGCCAGTAA TTCTAAACAA
    CTTAAGGGAA GTTCCCATCT GCCTTCCTTT TTAGAAAAGT CAGTTGTATT TTCCTCTGGT
    GAAAACATGG ACTTGACTGA AAACTCTGCA GTAGTGGCTC CTGATCAAAA CATCTGTACT
    ATTTTCCCAG GAAGAAAAGC AGCACCAGGA CGTATTGAAG ATAAAAGTAA AATATCCTTT
    AAAAAAGAGG TAATGATGAC AAACAGTCAG GAACAGCCTG GATGTGATAT GTACTCCTTG
    GTATCTGATA AGAAAATGTT AACTGTGACT GGTATAAAGC ATTTGCTTTC TGCAGATGAG
    AAAACTACCA TCTTTTCAGA AGGTGCTAAT ATGGACATCA CTGGAAATCA TACAGCTTCA
    GCAGACAACA AAACGATTTT GCAGCCTGAA ATTTTGAACA ATGACATATC CTTCATTTCT
    GAAGATAAAA CTTCTGTTTT TACAAAAAAT AACGACATGG AAATAAGTAG GCTTGATTCT
    GTTGCAACTG ATAATTCTAT GGAAAAGGCT GTATCTCAAG GGATGCTTAG CAGAACTAAT
    GGAGCTAGAA GGAAAAGCTT GAAGGGAACA ATAGGGGAAA AGACTGTTTT ATTTTCACTG
    AGTGAGAATA ATGACATGAG CATCACAGAG GGACTTACAG CAGCAATAAG TCATGAAATT
    GTCTCACAAA ATGAGGCAGG GGCTCCTTCT CCATCTTCAG CTCATCCTGT TACAAATGTT
    ACATTCACAT GTAACCAGGC TGACATTAAG TCTTGCTCTG CCAATAAAAT TACTGTAAAA
    GTTCCATGTG ATGATGCGTT GATCTCAGAT AAAGTTTTTG GACAGGAATT TCCTTCAAAC
    AGAGAAACTA CTGTGTTTGC TATGGATGAT ATGGAAATCA CTAAGGCCCA TAATGCATCT
    TTAAAAGAAA ATTTTCTGCA AAGTAAGCAA CCCAATCAAT CCGTTCCTTT AACTTCCTCA
    ATTATATTTA CAAGTGACCA GGCTGACATG GAAATGACTG AGTATCACCC TGTTGATGAA
    AGTGTTGAAA GGGATTTATG TGAGGATACA TTAAAGCTGC CCGCAGAGGT TCTACAGGAG
    GCTCTTTCAG GTAGCAAAAC AGTCATTTTT CCTTTGGATG AGAATATGGA CGTCACAAAA
    ACTCATGATG TATTAAGTGG TAAGATCAGT GTATGGAATG GAAGGTCCAT TCCAGCCATT
    TCTGCAGTTC CTGCTGATAA GACCATTGTA TTTCATCACA ACCAAGATGA TATGGAAATA
    ACTTCATCAC ATACTGTTTC AGTTAATAAC AACACTGAAG GATCTGAGAA TCAAAAAGCA
    TCACATAAAA GCACTCAACA ACCAGACTTG CGTAGCGTAT CATCATCCTC TTGCAGAGAT
    GAAATAGATT CTCTACAAAT AAAAGACCTG AATGGTGGGG ATCATAAAAA ATCTAATATT
    AAGTCCTCAA TTCCTTCTGC TTCATCGGCC TCAGCGTCAC CAACTGAGAA AGGACCTATC
    AAAGTCCACA GTGGCACAAT CCCAGATTCC ATTTATTCTA TCTCAATTCC AAAAGATGCT
    TTGGATGTCC AAGCACTACA GGATCTTGGC CTTTCCACAA AAAATTCAAT TCCTATAAAC
    AACGAGCAGG ATCTTTTACA TCCAGGAGCA ATGAAATCAA GGAGAGCATC TTTCAAGATG
    CCAGGTAATG ACCCCTTGGA CTGCAGTGAA GAAAGTGGAG TTGTAGTATC CCATGTTTTT
    CTTCCTGCTC AGCACTCAGA CTCTTTGAAG GATCCTCCAG ATGCACTTAG TACTCAGAGA
    AATCAAGTAT TGAAAGATGA TTCATCTAGA CCTGAAGATC TAGCCACAGA TTTAGCCATG
    GATGGAGCAG GCATTGTTCC AGTTTCTAAC AAGGAATCGG AGGAAAATGT GCTGTCAGCT
    GAAGGAGAGG CAACAGAATT CCAAATAAAC TCTGAACAAA CTAAGCAACT TTCAGTCAGC
    GATAGTCTGA GAGATCCAAT AGATCTTGCG CCTAAATTAT CAGGTATTAT AAATATTTGT
    TCAAAACTGG AAAATATTAG AAGGAAATCT GTAGTTGTTT CTGAAACTGT TCTTTCTGAC
    CACTTGCTGA AGTTACCAAC TCAGCCAGAG GATACCTTGA TGTTAGGAAG AAATAGCATA
    AATGAACAAA GGTTATGTTA TGCCAAAGAG CAGGAGAATG CATGTCTTGA ATCTGGAGCT
    GCTCCCATAG ATGCAAATTT AGGAATGGCA CTTAAGGACA AATATAAAGG AAGAAACTTC
    CCTCTAGGTA TCTTTCAGCC TAAACTACCA AACAGAAGAA ATCCTTCTGT TTCTAGTGTG
    CAAGACACAA CTGCAAAATC TTCAGGCAAA GTAGAATCAG CAGTTCCAGA AGTAAATCTG
    AACACCATTA AAGCTCCAGG TTACAACAAC GCTAGTAGGC AGAACTTCAG CCCTTCTCAG
    TTTATAGCAG AAGAATTTCT TCCTGTCTGC CCAGAAGAAA TGGATTCAAA TGATTCTGTA
    AGCTCTGAGT TTGTGGAAGA GGCTTGCAAT AACGGAAACA AAAGAGAAAT CCTCCACAAT
    GAAGAGATTC AAGTTGAAGA GACAAAAACT TGCAACAATG CAAAAAGAGC TTTGAAATGT
    GTTGAGGAGG ATCTTCAAAG TCTAAAGAGG CTCAAGATGG ATGAAATCGT GGTTGATGGG
    ACCTCCCAGG ACTTGCAGGT TACTTACGGC ACTGTACCTG AAAGCCAAGC AGAAGTTGCT
    GAAAGTGAAG ATTCTCCAAA TCTATCAGCT AAAAACCCTG ACTGTACACA TGCCAACAGC
    AGCAGCTCTC TAGATTCTGT TAAGGCTGAC ACAGAATTAA CAATTCAACG AAGCAGTCAA
    ATGGAATCGC AGCTTCTCAC AGATAGCATT TGTGAAGATA ATTTGTGGGA GAAATTTCAG
    AGTGGTGTCA TCACAGTTGC GGAGTTTTTT ACTCTTCTTC AAGTCCATAT TCCAATTCAG
    AAGCCCCGGC AGAGCCATAT TCCAGCCAAC TGTGCTGTCA GTGCTCCACC TACTCCAGAA
    GACCTGATTC TCAGCCAATA CGTTTATCGT CCCAAGATGC GTATTTATGA AGAAGACTGC
    CAGGTTCTTT CTCAGATGAT AGATGAATTA AAACAGTATG TAAGTGTCCA GGATCAACCA
    CTGGTGAATG TGAATAAGAG TTTGTGGGAA GTAATGAGAA CCTGCTCTGA CGAAGAGCTG
    AAGAGCTTTG GAGCAGAACT GAACAAAATG AAATCCCATT TCATCAAGGA AAGTAAAATC
    CTGGCTCACA ATGAGAAAGA GGCATTATAC AGCAAATTGC TGCAGAGTGC GCAGGAACAA
    TATGAAAAGC TCCAGTCAAG AACAGCAAAC ATGGATGAAT TGCTTAAGGA GGCAGAAAGT
    TGTCTTGCAG ATCTAAAAGC AGATTCTGAA TGGGAAGAAT GTGAAACAGA CTGCAGTGAT
    GATGAAATGA AGGGAGAGAA CTCAGAATCA AGAAAACTAG AAGAAGAATT GGGAAGCCTC
    AAAGCTCAAG AAGAGGAACT TCAAAGAGAA CTGTCAGATC TGGAGACTGA GAATGAACAA
    ATACTTGCTC AGATGAAGCA TCTACAGGAA GATGAAAAGA GCTGCCAGGA ACTCCTGGAG
    AGATGCAACT TCACTGAGTG GGAGATTAGT GAATGGAGTG AACTCCAGGC AGTCTTTAAC
    TTTCTTCATG ATTCTATTGA ACTCACAGTT GTGTTTGGAA CCCCAATAGA TGGTGATGTT
    TTTGGTGAAA ATCCTTCCAG AAAAATAGTT AGCCTGAGCT TTGAATCTCT CTTGGATGAG
    GAAAAAGCTC CACTCTCCTC ACGTTTAGTC CAAAGACTCA TATTCCAGTT TATTGAAAGT
    CAGGGATGCT GGCAGGAAAA GTGTCCCACA CTACAATACT TGCCTCAGGT GCTTCACGAT
    GTCTCTTTGG TGGTGAGTCG TTGTAGGATC TTGGGAGAGG AGATCGAATT TCTGGAGAGA
    TGGGGTGGAA AATACAATCT TCTCAAGACA GATATAATTG ACACAAATGT GAAGCTCCTC
    TTCTCCACTT CCACCGCTTT TGCAAAGTTT GAGTTGACAC TGTCTTTATC ATCCAACTAC
    CCATCTGCTT CACTGCCTTT CACTGTTGAA AAAAAAATTG GGAATATTGG ACATGAGGAA
    ATTTCTGCTG TTCTCTCTAA AGTACCTATT GGTTACCATT ATCTCCGAAG AATGGTGAGC
    TTGATTCATC AAAATCTGCT CCAGGACCCC AGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025006921.1
    CDS86..6901
    Translation

    Target ORF information:

    RefSeq Version XM_025151153.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus kinetochore scaffold 1 (KNL1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025151153.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGGGTGCTG TGGGGCTGGG GGTCAGCGGC GGAGAGCGGC CGAGAGTGTG CCATGATGTG 
    GACAATACTA TTCCGAGAAC AGATAGGCCT GATGCAACAG TCATCTTCTC AGATGAAAAT
    GAAATGGATA TGACAGCTAG TCACACTGCA GTGATTGCAC GTAACCTGAA TAACAGTGAA
    ACTGACAAAA CTGAGAAAAT AGATATTGCG TCATTTCTGG CTGAGTTAAA TTCTATCAAT
    GGAAAAGCAG AAAAAAACAA AGATTTTCAT TTTTCCTTTG ATGCTGCAGA CCGTTTTTGC
    CCATCTTTTG AACAGAAGGA AGATGCTACT ACAGGAAAGA AAATAAATTC CAATGAATTT
    TTCATGAACT CAAATGAAAA AGCACTTAAT CCTATTGAAG GACCTGAAAA AGAGAACCTT
    TTTTTTGTCC CTTCAGAAGT GTCAGAAAAC ATGGCACGAT CATCAGTGGA ATATGTATAT
    TCCCATGAAC AACTAGCCAC CTGCAATGTA ACAAAAATAT TTAGAGAGCA AGATGATGGA
    ATGGAAATAA CAAAGTGCCA AGCATCTGAT ATTCAAAATA TGCATTCTGG TCTTTCTGAG
    GTTCCGACAG AGCAGTTAGC ATCTGCAGAT GTTACTCAGG CATTTGCAGA TGATAGAATG
    GATATGACAA CTAATCACAC TGCAAAAATA CATTTTCCCT TTGCCATTGT AGGTAATCAA
    AGTTTAAATT CCAGAAAGGA TTTCTCATCC ATGGAGCTAG ATAACTACTG TATTGTAAAG
    AGGACATCTC ACCAGCAATT AATCCTAAAG CAAGACCCTC AACTTTGTAC ACGCAAAAAA
    ATCATAAATG TTGAAGACAC AGTAGATGCT ACAGCACTGC AGGCTTTTAA GCAGGAGAGC
    AGAACATTGT CTTCTATCCC AGGATCTGTT TCTTCTGAGA CTGTCTTCCG AGATGATAAA
    ACTGTTGTTT TTTCTCAATG TGATGACATG GAAATTACTG GGAATTACAG AGATGTACTC
    TATAATACAA GTACCAGAGG AATGAACAGT TCATGTAATA AAGCTTTTGA AAAAACAGTT
    AACAGAAGTT CATTGCTAGT AGAAAGACGA CATCCGGTAA ATAATGACAA TACAAGAAGT
    CTGTCTTTAG ATGATAGATC TTCTCCGTCA CGCAAGAATA GTGCACGTGA ACTATCTTCA
    GGTTCAGATG GACAAAATGA AAGAGTAACC CAAGATCACA GTACTGTTTC ATTGAACACT
    GGTTTCAATT CATGTACAAA TTCAATGTCT ACTGGTTCCT CTAAGGGTAG GCTGCAGCAC
    AGACTAGCAA ACTCCCTACT TGTTTCACTA CCAGGTGAGA AAACTGCAAT ATTTTCAGGA
    ACAGATGTGG ACTTGACTAA AAATTGTGTT GGCAAGGGTT ATAGAAAGAA TATTGAAAAT
    GAATTTCCTC CTGGATTGTC CAACTCTGTC ACTTGCAAAC CACATTTTCT TGATAGTAGG
    TCTGTATCTT CCAATTTAAG GGAGCAGGAA AAAATGGAAA TAACTAAATG TCAGACAGTT
    GTCAGTGGTG ATCAAAGCAT TGGGATAACC ACAGAAGCAG AACAAAGGCA TTGTAAAATA
    ATTCCAAGTA AGAACCAGAA CAATAATGTT AGTGATTCTT TGGATAGGGA CAAGGAAAAT
    CTTGAGGCAA TTGGTTTTGA TGTGAGTATT AGAAGAAGCC AGGCTATAGA AGCGAATGCT
    AAAGGTGTTG AGGTGGTAAC TGTCAATAAG AAGCCTGGAA AAACAGACTT TCAGGCTAGT
    AGTCTGAGTT CCTGTGCAAA GAGCAGGTAT TCACTCCAGG AGCAAAAGAG GGAAGTCTCT
    GAAAATATTG TCACTGACAA ATGTGCATTC ACATCTTTGC AAAGTGAAAA AGTATATGTA
    TCCCAGCCTT TGGGAAATAA ACTTCCAAAT TCTTCAGTTT CCTGCACAAA TGACAAAAGA
    GACATGTTTT CAGATGATGA AAACATGGAC ATAACCAAAG CTCATTCTTT GGTTGCTCCT
    TCTATGAATA TAGTACAAGA AAATGAGAAT ACTCACGTTC AAAATAATGT AAAAGCCAAA
    CAGCTGCAAA ACCAGACCTT CCAGTTTTCT GATAATTCTG GTATAGATCA TACAAGTCAG
    ACTGCAGCAA TAGAATGTGG AGATGTCAAA AGGAGCTCAA ATAAACAAAC TGTTGCTCAT
    TTAGCTCCAA GTGGTTTCTT TATTTCCAGT AATGAGCTTG CTCCCTCTGG AAAGAAAAGA
    GATGCACAAC CTGGAAATAC ACTGGGAATA ATCTCAGATT GCCAGAACTC TGATAGGCAT
    AAGAAAACAC ATCCTAGGAT AGTAGTAAAC AATGTATCAT CAACTGCAGA AGACCGTGAG
    AGTGATTTTT TGATCAGTAA AACAGTTTCT TTAGAAGGGG GTTTTAATAA CCCTCAGTCA
    GGTGATGACC TGTGCTTGAG GGGTGTTGAA GAGAGGTCAC TAGCCAGTAA TTCTAAACAA
    CTTAAGGGAA GTTCCCATCT GCCTTCCTTT TTAGAAAAGT CAGTTGTATT TTCCTCTGGT
    GAAAACATGG ACTTGACTGA AAACTCTGCA GTAGTGGCTC CTGATCAAAA CATCTGTACT
    ATTTTCCCAG GAAGAAAAGC AGCACCAGGA CGTATTGAAG ATAAAAGTAA AATATCCTTT
    AAAAAAGAGG TAATGATGAC AAACAGTCAG GAACAGCCTG GATGTGATAT GTACTCCTTG
    GTATCTGATA AGAAAATGTT AACTGTGACT GGTATAAAGC ATTTGCTTTC TGCAGATGAG
    AAAACTACCA TCTTTTCAGA AGGTGCTAAT ATGGACATCA CTGGAAATCA TACAGCTTCA
    GCAGACAACA AAACGATTTT GCAGCCTGAA ATTTTGAACA ATGACATATC CTTCATTTCT
    GAAGATAAAA CTTCTGTTTT TACAAAAAAT AACGACATGG AAATAAGTAG GCTTGATTCT
    GTTGCAACTG ATAATTCTAT GGAAAAGGCT GTATCTCAAG GGATGCTTAG CAGAACTAAT
    GGAGCTAGAA GGAAAAGCTT GAAGGGAACA ATAGGGGAAA AGACTGTTTT ATTTTCACTG
    AGTGAGAATA ATGACATGAG CATCACAGAG GGACTTACAG CAGCAATAAG TCATGAAATT
    GTCTCACAAA ATGAGGCAGG GGCTCCTTCT CCATCTTCAG CTCATCCTGT TACAAATGTT
    ACATTCACAT GTAACCAGGC TGACATTAAG TCTTGCTCTG CCAATAAAAT TACTGTAAAA
    GTTCCATGTG ATGATGCGTT GATCTCAGAT AAAGTTTTTG GACAGGAATT TCCTTCAAAC
    AGAGAAACTA CTGTGTTTGC TATGGATGAT ATGGAAATCA CTAAGGCCCA TAATGCATCT
    TTAAAAGAAA ATTTTCTGCA AAGTAAGCAA CCCAATCAAT CCGTTCCTTT AACTTCCTCA
    ATTATATTTA CAAGTGACCA GGCTGACATG GAAATGACTG AGTATCACCC TGTTGATGAA
    AGTGTTGAAA GGGATTTATG TGAGGATACA TTAAAGCTGC CCGCAGAGGT TCTACAGGAG
    GCTCTTTCAG GTAGCAAAAC AGTCATTTTT CCTTTGGATG AGAATATGGA CGTCACAAAA
    ACTCATGATG TATTAAGTGG TAAGATCAGT GTATGGAATG GAAGGTCCAT TCCAGCCATT
    TCTGCAGTTC CTGCTGATAA GACCATTGTA TTTCATCACA ACCAAGATGA TATGGAAATA
    ACTTCATCAC ATACTGTTTC AGTTAATAAC AACACTGAAG GATCTGAGAA TCAAAAAGCA
    TCACATAAAA GCACTCAACA ACCAGACTTG CGTAGCGTAT CATCATCCTC TTGCAGAGAT
    GAAATAGATT CTCTACAAAT AAAAGACCTG AATGGTGGGG ATCATAAAAA ATCTAATATT
    AAGTCCTCAA TTCCTTCTGC TTCATCGGCC TCAGCGTCAC CAACTGAGAA AGGACCTATC
    AAAGTCCACA GTGGCACAAT CCCAGATTCC ATTTATTCTA TCTCAATTCC AAAAGATGCT
    TTGGATGTCC AAGCACTACA GGATCTTGGC CTTTCCACAA AAAATTCAAT TCCTATAAAC
    AACGAGCAGG ATCTTTTACA TCCAGGAGCA ATGAAATCAA GGAGAGCATC TTTCAAGATG
    CCAGGTAATG ACCCCTTGGA CTGCAGTGAA GAAAGTGGAG TTGTAGTATC CCATGTTTTT
    CTTCCTGCTC AGCACTCAGA CTCTTTGAAG GATCCTCCAG ATGCACTTAG TACTCAGAGA
    AATCAAGTAT TGAAAGATGA TTCATCTAGA CCTGAAGATC TAGCCACAGA TTTAGCCATG
    GATGGAGCAG GCATTGTTCC AGTTTCTAAC AAGGAATCGG AGGAAAATGT GCTGTCAGCT
    GAAGGAGAGG CAACAGAATT CCAAATAAAC TCTGAACAAA CTAAGCAACT TTCAGTCAGC
    GATAGTCTGA GAGATCCAAT AGATCTTGCG CCTAAATTAT CAGGTATTAT AAATATTTGT
    TCAAAACTGG AAAATATTAG AAGGAAATCT GTAGTTGTTT CTGAAACTGT TCTTTCTGAC
    CACTTGCTGA AGTTACCAAC TCAGCCAGAG GATACCTTGA TGTTAGGAAG AAATAGCATA
    AATGAACAAA GGTTATGTTA TGCCAAAGAG CAGGAGAATG CATGTCTTGA ATCTGGAGCT
    GCTCCCATAG ATGCAAATTT AGGAATGGCA CTTAAGGACA AATATAAAGG AAGAAACTTC
    CCTCTAGGTA TCTTTCAGCC TAAACTACCA AACAGAAGAA ATCCTTCTGT TTCTAGTGTG
    CAAGACACAA CTGCAAAATC TTCAGGCAAA GTAGAATCAG CAGTTCCAGA AGTAAATCTG
    AACACCATTA AAGCTCCAGG TTACAACAAC GCTAGTAGGC AGAACTTCAG CCCTTCTCAG
    TTTATAGCAG AAGAATTTCT TCCTGTCTGC CCAGAAGAAA TGGATTCAAA TGATTCTGTA
    AGCTCTGAGT TTGTGGAAGA GGCTTGCAAT AACGGAAACA AAAGAGAAAT CCTCCACAAT
    GAAGAGATTC AAGTTGAAGA GACAAAAACT TGCAACAATG CAAAAAGAGC TTTGAAATGT
    GTTGAGGAGG ATCTTCAAAG TCTAAAGAGG CTCAAGATGG ATGAAATCGT GGTTGATGGG
    ACCTCCCAGG ACTTGCAGGT TACTTACGGC ACTGTACCTG AAAGCCAAGC AGAAGTTGCT
    GAAAGTGAAG ATTCTCCAAA TCTATCAGCT AAAAACCCTG ACTGTACACA TGCCAACAGC
    AGCAGCTCTC TAGATTCTGT TAAGGCTGAC ACAGAATTAA CAATTCAACG AAGCAGTCAA
    ATGGAATCGC AGCTTCTCAC AGATAGCATT TGTGAAGATA ATTTGTGGGA GAAATTTCAG
    AGTGGTGTCA TCACAGTTGC GGAGTTTTTT ACTCTTCTTC AAGTCCATAT TCCAATTCAG
    AAGCCCCGGC AGAGCCATAT TCCAGCCAAC TGTGCTGTCA GTGCTCCACC TACTCCAGAA
    GACCTGATTC TCAGCCAATA CGTTTATCGT CCCAAGATGC GTATTTATGA AGAAGACTGC
    CAGGTTCTTT CTCAGATGAT AGATGAATTA AAACAGTATG TAAGTGTCCA GGATCAACCA
    CTGGTGAATG TGAATAAGAG TTTGTGGGAA GTAATGAGAA CCTGCTCTGA CGAAGAGCTG
    AAGAGCTTTG GAGCAGAACT GAACAAAATG AAATCCCATT TCATCAAGGA AAGTAAAATC
    CTGGCTCACA ATGAGAAAGA GGCATTATAC AGCAAATTGC TGCAGAGTGC GCAGGAACAA
    TATGAAAAGC TCCAGTCAAG AACAGCAAAC ATGGATGAAT TGCTTAAGGA GGCAGAAAGT
    TGTCTTGCAG ATCTAAAAGC AGATTCTGAA TGGGAAGAAT GTGAAACAGA CTGCAGTGAT
    GATGAAATGA AGGGAGAGAA CTCAGAATCA AGAAAACTAG AAGAAGAATT GGGAAGCCTC
    AAAGCTCAAG AAGAGGAACT TCAAAGAGAA CTGTCAGATC TGGAGACTGA GAATGAACAA
    ATACTTGCTC AGATGAAGCA TCTACAGGAA GATGAAAAGA GCTGCCAGGA ACTCCTGGAG
    AGATGCAACT TCACTGAGTG GGAGATTAGT GAATGGAGTG AACTCCAGGC AGTCTTTAAC
    TTTCTTCATG ATTCTATTGA ACTCACAGTT GTGTTTGGAA CCCCAATAGA TGGTGATGTT
    TTTGGTGAAA ATCCTTCCAG AAAAATAGTT AGCCTGAGCT TTGAATCTCT CTTGGATGAG
    GAAAAAGCTC CACTCTCCTC ACGTTTAGTC CAAAGACTCA TATTCCAGTT TATTGAAAGT
    CAGGGATGCT GGCAGGAAAA GTGTCCCACA CTACAATACT TGCCTCAGGT GCTTCACGAT
    GTCTCTTTGG TGGTGAGTCG TTGTAGGATC TTGGGAGAGG AGATCGAATT TCTGGAGAGA
    TGGGGTGGAA AATACAATCT TCTCAAGACA GATATAATTG ACACAAATGT GAAGCTCCTC
    TTCTCCACTT CCACCGCTTT TGCAAAGTTT GAGTTGACAC TGTCTTTATC ATCCAACTAC
    CCATCTGCTT CACTGCCTTT CACTGTTGAA AAAAAAATTG GGAATATTGG ACATGAGGAA
    ATTTCTGCTG TTCTCTCTAA AGTACCTATT GGTTACCATT ATCTCCGAAG AATGGTGAGC
    TTGATTCATC AAAATCTGCT CCAGGACCCC AGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa36086
    Clone ID Related Accession (Same CDS sequence) XM_015286271.1
    Accession Version XM_015286271.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6033bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product kinetochore scaffold 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGCTGTACA GCGCTGCCGC CAGGAAGGCG GTTTGGCGCG GCGCGCAGGC GCGGCGGTTA 
    GGTTTGAATT GTGAGGCGGC AGCGGCGGCC CAGTCCTATG GGTGCTGTGG GGCTGGGGGT
    CAGCGGCGGA GAGCGGCCGA GAGTGTACTG TTTTTTATAG GAGAAACACT TCTTGCAGAA
    ATGGACAAAA TTTACATGGA CTCTAACGTG GAGAACGATA ACACAGAGCA CATCAGAGGA
    AAACGACTGT CCTCTATATT AAAGGCTCCA CGAAATCCTC TTGATGATCT GGGAAATGGG
    AATGAACTCA CCCAGGATCT CAACATAGAA AAACGTAGGA AAAGCTCTCG ACGTGTCAGC
    TTTGCCAACA CCATAAACTG CAGGGTCTTC CATCGGGATC TTAAGAATAA TGCAGCAGAA
    AGAGAAAATG AAGCAGAAGA TACGGAGAAT CATGTCCTGC TTAATCAAAA TGAAGAACCT
    GAAGCTGTTC CATGTGAGAT TACTGGGATG AACACCTTGC TTCATGCCCC TATTCAGTCA
    TTGGTGCAGC AGACTGATGA GTGCCATGAT GTGGACAATA CTATTCCGAG AACAGATAGG
    CCTGATGCAA CAGTCATCTT CTCAGATGAA AATGAAATGG ATATGACAGC TAGTCACACT
    GCAGTGATTG CACGTAACCT GAATAACAGT GAAACTGACA AAACTGAGAA AATAGATATT
    GCGTCATTTC TGGCTGAGTT AAATTCTATC AATGGAAAAG CAGAAAAAAA CAAAGATTTT
    CATTTTTCCT TTGATGCTGC AGACCGTTTT TGCCCATCTT TTGAACAGAA GGAAGATGCT
    ACTACAGGAA AGAAAATAAA TTCCAATGAA TTTTTCATGA ACTCAAATGA AAAAGCACTT
    AATCCTATTG AAGGACCTGA AAAAGAGAAC CTTTTTTTTG TCCCTTCAGA AGTGTCAGAA
    AACATGGCAC GATCATCAGT GGAATATGTA TATTCCCATG AACAACTAGC CACCTGCAAT
    GTAACAAAAA TATTTAGAGA GCAAGATGAT GGAATGGAAA TAACAAAGTG CCAAGCATCT
    GATATTCAAA ATATGCATTC TGGTCTTTCT GAGGTTCCGA CAGAGCAGTT AGCATCTGCA
    GATGTTACTC AGGCATTTGC AGATGATAGA ATGGATATGA CAACTAATCA CACTGCAAAA
    ATACATTTTC CCTTTGCCAT TGTAGGTAAT CAAAGTTTAA ATTCCAGAAA GGATTTCTCA
    TCCATGGAGC TAGATAACTA CTGTATTGTA AAGAGGACAT CTCACCAGCA ATTAATCCTA
    AAGCAAGACC CTCAACTTTG TACACGCAAA AAAATCATAA ATGTTGAAGA CACAGTAGAT
    GCTACAGCAC TGCAGGCTTT TAAGCAGGAG AGCAGAACAT TGTCTTCTAT CCCAGGATCT
    GTTTCTTCTG AGACTGTCTT CCGAGATGAT AAAACTGTTG TTTTTTCTCA ATGTGATGAC
    ATGGAAATTA CTGGGAATTA CAGAGATGTA CTCTATAATA CAAGTACCAG AGGAATGAAC
    AGTTCATGTA ATAAAGCTTT TGAAAAAACA GTTAACAGAA GTTCATTGCT AGTAGAAAGA
    CGACATCCGG TAAATAATGA CAATACAAGA AGTCTGTCTT TAGATGATAG ATCTTCTCCG
    TCACGCAAGA ATAGTGCACG TGAACTATCT TCAGGTTCAG ATGGACAAAA TGAAAGAGTA
    ACCCAAGATC ACAGTACTGT TTCATTGAAC ACTGGTTTCA ATTCATGTAC AAATTCAATG
    TCTACTGGTT CCTCTAAGGG TAGGCTGCAG CACAGACTAG CAAACTCCCT ACTTGTTTCA
    CTACCAGGAA GAAAAGCAGC ACCAGGACGT ATTGAAGATA AAAGTAAAAT ATCCTTTAAA
    AAAGAGGTAA TGATGACAAA CAGTCAGGAA CAGCCTGGAT GTGATATGTA CTCCTTGGTA
    TCTGATAAGA AAATGTTAAC TGTGACTGGT ATAAAGCATT TGCTTTCTGC AGATGAGAAA
    ACTACCATCT TTTCAGAAGG TGCTAATATG GACATCACTG GAAATCATAC AGCTTCAGCA
    GACAACAAAA CGATTTTGCA GCCTGAAATT TTGAACAATG ACATATCCTT CATTTCTGAA
    GATAAAACTT CTGTTTTTAC AAAAAATAAC GACATGGAAA TAAGTAGGCT TGATTCTGTT
    GCAACTGATA ATTCTATGGA AAAGGCTGTA TCTCAAGGGA TGCTTAGCAG AACTAATGGA
    GCTAGAAGGA AAAGCTTGAA GGGAACAATA GGGGAAAAGA CTGTTTTATT TTCACTGAGT
    GAGAATAATG ACATGAGCAT CACAGAGGGA CTTACAGCAG CAATAAGTCA TGAAATTGTC
    TCACAAAATG AGGCAGGGGC TCCTTCTCCA TCTTCAGCTC ATCCTGTTAC AAATGTTACA
    TTCACATGTA ACCAGGCTGA CATTAAGTCT TGCTCTGCCA ATAAAATTAC TGTAAAAGTT
    CCATGTGATG ATGCGTTGAT CTCAGATAAA GTTTTTGGAC AGGAATTTCC TTCAAACAGA
    GAAACTACTG TGTTTGCTAT GGATGATATG GAAATCACTA AGGCCCATAA TGCATCTTTA
    AAAGAAAATT TTCTGCAAAG TAAGCAACCC AATCAATCCG TTCCTTTAAC TTCCTCAATT
    ATATTTACAA GTGACCAGGC TGACATGGAA ATGACTGAGT ATCACCCTGT TGATGAAAGT
    GTTGAAAGGG ATTTATGTGA GGATACATTA AAGCTGCCCG CAGAGGTTCT ACAGGAGGCT
    CTTTCAGGTA GCAAAACAGT CATTTTTCCT TTGGATGAGA ATATGGACGT CACAAAAACT
    CATGATGTAT TAAGTGGTAA GATCAGTGTA TGGAATGGAA GGTCCATTCC AGCCATTTCT
    GCAGTTCCTG CTGATAAGAC CATTGTATTT CATCACAACC AAGATGATAT GGAAATAACT
    TCATCACATA CTGTTTCAGT TAATAACAAC ACTGAAGGAT CTGAGAATCA AAAAGCATCA
    CATAAAAGCA CTCAACAACC AGACTTGCGT AGCGTATCAT CATCCTCTTG CAGAGATGAA
    ATAGATTCTC TACAAATAAA AGACCTGAAT GGTGGGGATC ATAAAAAATC TAATATTAAG
    TCCTCAATTC CTTCTGCTTC ATCGGCCTCA GCGTCACCAA CTGAGAAAGG ACCTATCAAA
    GTCCACAGTG GCACAATCCC AGATTCCATT TATTCTATCT CAATTCCAAA AGATGCTTTG
    GATGTCCAAG CACTACAGGA TCTTGGCCTT TCCACAAAAA ATTCAATTCC TATAAACAAC
    GAGCAGGATC TTTTACATCC AGGAGCAATG AAATCAAGGA GAGCATCTTT CAAGATGCCA
    GGTAATGACC CCTTGGACTG CAGTGAAGAA AGTGGAGTTG TAGTATCCCA TGTTTTTCTT
    CCTGCTCAGC ACTCAGACTC TTTGAAGGAT CCTCCAGATG CACTTAGTAC TCAGAGAAAT
    CAAGTATTGA AAGATGATTC ATCTAGACCT GAAGATCTAG CCACAGATTT AGCCATGGAT
    GGAGCAGGCA TTGTTCCAGT TTCTAACAAG GAATCGGAGG AAAATGTGCT GTCAGCTGAA
    GGAGAGGCAA CAGAATTCCA AATAAACTCT GAACAAACTA AGCAACTTTC AGTCAGCGAT
    AGTCTGAGAG ATCCAATAGA TCTTGCGCCT AAATTATCAG GTATTATAAA TATTTGTTCA
    AAACTGGAAA ATATTAGAAG GAAATCTGTA GTTGTTTCTG AAACTGTTCT TTCTGACCAC
    TTGCTGAAGT TACCAACTCA GCCAGAGGAT ACCTTGATGT TAGGAAGAAA TAGCATAAAT
    GAACAAAGGT TATGTTATGC CAAAGAGCAG GAGAATGCAT GTCTTGAATC TGGAGCTGCT
    CCCATAGATG CAAATTTAGG AATGGCACTT AAGGACAAAT ATAAAGGAAG AAACTTCCCT
    CTAGGTATCT TTCAGCCTAA ACTACCAAAC AGAAGAAATC CTTCTGTTTC TAGTGTGCAA
    GACACAACTG CAAAATCTTC AGGCAAAGTA GAATCAGCAG TTCCAGAAGT AAATCTGAAC
    ACCATTAAAG CTCCAGGTTA CAACAACGCT AGTAGGCAGA ACTTCAGCCC TTCTCAGTTT
    ATAGCAGAAG AATTTCTTCC TGTCTGCCCA GAAGAAATGG ATTCAAATGA TTCTGTAAGC
    TCTGAGTTTG TGGAAGAGGC TTGCAATAAC GGAAACAAAA GAGAAATCCT CCACAATGAA
    GAGATTCAAG TTGAAGAGAC AAAAACTTGC AACAATGCAA AAAGAGCTTT GAAATGTGTT
    GAGGAGGATC TTCAAAGTCT AAAGAGGCTC AAGATGGATG AAATCGTGGT TGATGGGACC
    TCCCAGGACT TGCAGGTTAC TTACGGCACT GTACCTGAAA GCCAAGCAGA AGTTGCTGAA
    AGTGAAGATT CTCCAAATCT ATCAGCTAAA AACCCTGACT GTACACATGC CAACAGCAGC
    AGCTCTCTAG ATTCTGTTAA GGCTGACACA GAATTAACAA TTCAACGAAG CAGTCAAATG
    GAATCGCAGC TTCTCACAGA TAGCATTTGT GAAGATAATT TGTGGGAGAA ATTTCAGAGT
    GGTGTCATCA CAGTTGCGGA GTTTTTTACT CTTCTTCAAG TCCATATTCC AATTCAGAAG
    CCCCGGCAGA GCCATATTCC AGCCAACTGT GCTGTCAGTG CTCCACCTAC TCCAGAAGAC
    CTGATTCTCA GCCAATACGT TTATCGTCCC AAGATGCGTA TTTATGAAGA AGACTGCCAG
    GTTCTTTCTC AGATGATAGA TGAATTAAAA CAGTATGTAA GTGTCCAGGA TCAACCACTG
    GTGAATGTGA ATAAGAGTTT GTGGGAAGTA ATGAGAACCT GCTCTGACGA AGAGCTGAAG
    AGCTTTGGAG CAGAACTGAA CAAAATGAAA TCCCATTTCA TCAAGGAAAG TAAAATCCTG
    GCTCACAATG AGAAAGAGGC ATTATACAGC AAATTGCTGC AGAGTGCGCA GGAACAATAT
    GAAAAGCTCC AGTCAAGAAC AGCAAACATG GATGAATTGC TTAAGGAGGC AGAAAGTTGT
    CTTGCAGATC TAAAAGCAGA TTCTGAATGG GAAGAATGTG AAACAGACTG CAGTGATGAT
    GAAATGAAGG GAGAGAACTC AGAATCAAGA AAACTAGAAG AAGAATTGGG AAGCCTCAAA
    GCTCAAGAAG AGGAACTTCA AAGAGAACTG TCAGATCTGG AGACTGAGAA TGAACAAATA
    CTTGCTCAGA TGAAGCATCT ACAGGAAGAT GAAAAGAGCT GCCAGGAACT CCTGGAGAGA
    TGCAACTTCA CTGAGTGGGA GATTAGTGAA TGGAGTGAAC TCCAGGCAGT CTTTAACTTT
    CTTCATGATT CTATTGAACT CACAGTTGTG TTTGGAACCC CAATAGATGG TGATGTTTTT
    GGTGAAAATC CTTCCAGAAA AATAGTTAGC CTGAGCTTTG AATCTCTCTT GGATGAGGAA
    AAAGCTCCAC TCTCCTCACG TTTAGTCCAA AGACTCATAT TCCAGTTTAT TGAAAGTCAG
    GGATGCTGGC AGGAAAAGTG TCCCACACTA CAATACTTGC CTCAGGTGCT TCACGATGTC
    TCTTTGGTGG TGAGTCGTTG TAGGATCTTG GGAGAGGAGA TCGAATTTCT GGAGAGATGG
    GGTGGAAAAT ACAATCTTCT CAAGACAGAT ATAATTGACA CAAATGTGAA GCTCCTCTTC
    TCCACTTCCA CCGCTTTTGC AAAGTTTGAG TTGACACTGT CTTTATCATC CAACTACCCA
    TCTGCTTCAC TGCCTTTCAC TGTTGAAAAA AAAATTGGGA ATATTGGACA TGAGGAAATT
    TCTGCTGTTC TCTCTAAAGT ACCTATTGGT TACCATTATC TCCGAAGAAT GGTGAGCTTG
    ATTCATCAAA ATCTGCTCCA GGACCCCAGA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015141757.1
    CDS16..6048
    Translation

    Target ORF information:

    RefSeq Version XM_015286271.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus kinetochore scaffold 1 (KNL1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015286271.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGCTGTACA GCGCTGCCGC CAGGAAGGCG GTTTGGCGCG GCGCGCAGGC GCGGCGGTTA 
    GGTTTGAATT GTGAGGCGGC AGCGGCGGCC CAGTCCTATG GGTGCTGTGG GGCTGGGGGT
    CAGCGGCGGA GAGCGGCCGA GAGTGTACTG TTTTTTATAG GAGAAACACT TCTTGCAGAA
    ATGGACAAAA TTTACATGGA CTCTAACGTG GAGAACGATA ACACAGAGCA CATCAGAGGA
    AAACGACTGT CCTCTATATT AAAGGCTCCA CGAAATCCTC TTGATGATCT GGGAAATGGG
    AATGAACTCA CCCAGGATCT CAACATAGAA AAACGTAGGA AAAGCTCTCG ACGTGTCAGC
    TTTGCCAACA CCATAAACTG CAGGGTCTTC CATCGGGATC TTAAGAATAA TGCAGCAGAA
    AGAGAAAATG AAGCAGAAGA TACGGAGAAT CATGTCCTGC TTAATCAAAA TGAAGAACCT
    GAAGCTGTTC CATGTGAGAT TACTGGGATG AACACCTTGC TTCATGCCCC TATTCAGTCA
    TTGGTGCAGC AGACTGATGA GTGCCATGAT GTGGACAATA CTATTCCGAG AACAGATAGG
    CCTGATGCAA CAGTCATCTT CTCAGATGAA AATGAAATGG ATATGACAGC TAGTCACACT
    GCAGTGATTG CACGTAACCT GAATAACAGT GAAACTGACA AAACTGAGAA AATAGATATT
    GCGTCATTTC TGGCTGAGTT AAATTCTATC AATGGAAAAG CAGAAAAAAA CAAAGATTTT
    CATTTTTCCT TTGATGCTGC AGACCGTTTT TGCCCATCTT TTGAACAGAA GGAAGATGCT
    ACTACAGGAA AGAAAATAAA TTCCAATGAA TTTTTCATGA ACTCAAATGA AAAAGCACTT
    AATCCTATTG AAGGACCTGA AAAAGAGAAC CTTTTTTTTG TCCCTTCAGA AGTGTCAGAA
    AACATGGCAC GATCATCAGT GGAATATGTA TATTCCCATG AACAACTAGC CACCTGCAAT
    GTAACAAAAA TATTTAGAGA GCAAGATGAT GGAATGGAAA TAACAAAGTG CCAAGCATCT
    GATATTCAAA ATATGCATTC TGGTCTTTCT GAGGTTCCGA CAGAGCAGTT AGCATCTGCA
    GATGTTACTC AGGCATTTGC AGATGATAGA ATGGATATGA CAACTAATCA CACTGCAAAA
    ATACATTTTC CCTTTGCCAT TGTAGGTAAT CAAAGTTTAA ATTCCAGAAA GGATTTCTCA
    TCCATGGAGC TAGATAACTA CTGTATTGTA AAGAGGACAT CTCACCAGCA ATTAATCCTA
    AAGCAAGACC CTCAACTTTG TACACGCAAA AAAATCATAA ATGTTGAAGA CACAGTAGAT
    GCTACAGCAC TGCAGGCTTT TAAGCAGGAG AGCAGAACAT TGTCTTCTAT CCCAGGATCT
    GTTTCTTCTG AGACTGTCTT CCGAGATGAT AAAACTGTTG TTTTTTCTCA ATGTGATGAC
    ATGGAAATTA CTGGGAATTA CAGAGATGTA CTCTATAATA CAAGTACCAG AGGAATGAAC
    AGTTCATGTA ATAAAGCTTT TGAAAAAACA GTTAACAGAA GTTCATTGCT AGTAGAAAGA
    CGACATCCGG TAAATAATGA CAATACAAGA AGTCTGTCTT TAGATGATAG ATCTTCTCCG
    TCACGCAAGA ATAGTGCACG TGAACTATCT TCAGGTTCAG ATGGACAAAA TGAAAGAGTA
    ACCCAAGATC ACAGTACTGT TTCATTGAAC ACTGGTTTCA ATTCATGTAC AAATTCAATG
    TCTACTGGTT CCTCTAAGGG TAGGCTGCAG CACAGACTAG CAAACTCCCT ACTTGTTTCA
    CTACCAGGAA GAAAAGCAGC ACCAGGACGT ATTGAAGATA AAAGTAAAAT ATCCTTTAAA
    AAAGAGGTAA TGATGACAAA CAGTCAGGAA CAGCCTGGAT GTGATATGTA CTCCTTGGTA
    TCTGATAAGA AAATGTTAAC TGTGACTGGT ATAAAGCATT TGCTTTCTGC AGATGAGAAA
    ACTACCATCT TTTCAGAAGG TGCTAATATG GACATCACTG GAAATCATAC AGCTTCAGCA
    GACAACAAAA CGATTTTGCA GCCTGAAATT TTGAACAATG ACATATCCTT CATTTCTGAA
    GATAAAACTT CTGTTTTTAC AAAAAATAAC GACATGGAAA TAAGTAGGCT TGATTCTGTT
    GCAACTGATA ATTCTATGGA AAAGGCTGTA TCTCAAGGGA TGCTTAGCAG AACTAATGGA
    GCTAGAAGGA AAAGCTTGAA GGGAACAATA GGGGAAAAGA CTGTTTTATT TTCACTGAGT
    GAGAATAATG ACATGAGCAT CACAGAGGGA CTTACAGCAG CAATAAGTCA TGAAATTGTC
    TCACAAAATG AGGCAGGGGC TCCTTCTCCA TCTTCAGCTC ATCCTGTTAC AAATGTTACA
    TTCACATGTA ACCAGGCTGA CATTAAGTCT TGCTCTGCCA ATAAAATTAC TGTAAAAGTT
    CCATGTGATG ATGCGTTGAT CTCAGATAAA GTTTTTGGAC AGGAATTTCC TTCAAACAGA
    GAAACTACTG TGTTTGCTAT GGATGATATG GAAATCACTA AGGCCCATAA TGCATCTTTA
    AAAGAAAATT TTCTGCAAAG TAAGCAACCC AATCAATCCG TTCCTTTAAC TTCCTCAATT
    ATATTTACAA GTGACCAGGC TGACATGGAA ATGACTGAGT ATCACCCTGT TGATGAAAGT
    GTTGAAAGGG ATTTATGTGA GGATACATTA AAGCTGCCCG CAGAGGTTCT ACAGGAGGCT
    CTTTCAGGTA GCAAAACAGT CATTTTTCCT TTGGATGAGA ATATGGACGT CACAAAAACT
    CATGATGTAT TAAGTGGTAA GATCAGTGTA TGGAATGGAA GGTCCATTCC AGCCATTTCT
    GCAGTTCCTG CTGATAAGAC CATTGTATTT CATCACAACC AAGATGATAT GGAAATAACT
    TCATCACATA CTGTTTCAGT TAATAACAAC ACTGAAGGAT CTGAGAATCA AAAAGCATCA
    CATAAAAGCA CTCAACAACC AGACTTGCGT AGCGTATCAT CATCCTCTTG CAGAGATGAA
    ATAGATTCTC TACAAATAAA AGACCTGAAT GGTGGGGATC ATAAAAAATC TAATATTAAG
    TCCTCAATTC CTTCTGCTTC ATCGGCCTCA GCGTCACCAA CTGAGAAAGG ACCTATCAAA
    GTCCACAGTG GCACAATCCC AGATTCCATT TATTCTATCT CAATTCCAAA AGATGCTTTG
    GATGTCCAAG CACTACAGGA TCTTGGCCTT TCCACAAAAA ATTCAATTCC TATAAACAAC
    GAGCAGGATC TTTTACATCC AGGAGCAATG AAATCAAGGA GAGCATCTTT CAAGATGCCA
    GGTAATGACC CCTTGGACTG CAGTGAAGAA AGTGGAGTTG TAGTATCCCA TGTTTTTCTT
    CCTGCTCAGC ACTCAGACTC TTTGAAGGAT CCTCCAGATG CACTTAGTAC TCAGAGAAAT
    CAAGTATTGA AAGATGATTC ATCTAGACCT GAAGATCTAG CCACAGATTT AGCCATGGAT
    GGAGCAGGCA TTGTTCCAGT TTCTAACAAG GAATCGGAGG AAAATGTGCT GTCAGCTGAA
    GGAGAGGCAA CAGAATTCCA AATAAACTCT GAACAAACTA AGCAACTTTC AGTCAGCGAT
    AGTCTGAGAG ATCCAATAGA TCTTGCGCCT AAATTATCAG GTATTATAAA TATTTGTTCA
    AAACTGGAAA ATATTAGAAG GAAATCTGTA GTTGTTTCTG AAACTGTTCT TTCTGACCAC
    TTGCTGAAGT TACCAACTCA GCCAGAGGAT ACCTTGATGT TAGGAAGAAA TAGCATAAAT
    GAACAAAGGT TATGTTATGC CAAAGAGCAG GAGAATGCAT GTCTTGAATC TGGAGCTGCT
    CCCATAGATG CAAATTTAGG AATGGCACTT AAGGACAAAT ATAAAGGAAG AAACTTCCCT
    CTAGGTATCT TTCAGCCTAA ACTACCAAAC AGAAGAAATC CTTCTGTTTC TAGTGTGCAA
    GACACAACTG CAAAATCTTC AGGCAAAGTA GAATCAGCAG TTCCAGAAGT AAATCTGAAC
    ACCATTAAAG CTCCAGGTTA CAACAACGCT AGTAGGCAGA ACTTCAGCCC TTCTCAGTTT
    ATAGCAGAAG AATTTCTTCC TGTCTGCCCA GAAGAAATGG ATTCAAATGA TTCTGTAAGC
    TCTGAGTTTG TGGAAGAGGC TTGCAATAAC GGAAACAAAA GAGAAATCCT CCACAATGAA
    GAGATTCAAG TTGAAGAGAC AAAAACTTGC AACAATGCAA AAAGAGCTTT GAAATGTGTT
    GAGGAGGATC TTCAAAGTCT AAAGAGGCTC AAGATGGATG AAATCGTGGT TGATGGGACC
    TCCCAGGACT TGCAGGTTAC TTACGGCACT GTACCTGAAA GCCAAGCAGA AGTTGCTGAA
    AGTGAAGATT CTCCAAATCT ATCAGCTAAA AACCCTGACT GTACACATGC CAACAGCAGC
    AGCTCTCTAG ATTCTGTTAA GGCTGACACA GAATTAACAA TTCAACGAAG CAGTCAAATG
    GAATCGCAGC TTCTCACAGA TAGCATTTGT GAAGATAATT TGTGGGAGAA ATTTCAGAGT
    GGTGTCATCA CAGTTGCGGA GTTTTTTACT CTTCTTCAAG TCCATATTCC AATTCAGAAG
    CCCCGGCAGA GCCATATTCC AGCCAACTGT GCTGTCAGTG CTCCACCTAC TCCAGAAGAC
    CTGATTCTCA GCCAATACGT TTATCGTCCC AAGATGCGTA TTTATGAAGA AGACTGCCAG
    GTTCTTTCTC AGATGATAGA TGAATTAAAA CAGTATGTAA GTGTCCAGGA TCAACCACTG
    GTGAATGTGA ATAAGAGTTT GTGGGAAGTA ATGAGAACCT GCTCTGACGA AGAGCTGAAG
    AGCTTTGGAG CAGAACTGAA CAAAATGAAA TCCCATTTCA TCAAGGAAAG TAAAATCCTG
    GCTCACAATG AGAAAGAGGC ATTATACAGC AAATTGCTGC AGAGTGCGCA GGAACAATAT
    GAAAAGCTCC AGTCAAGAAC AGCAAACATG GATGAATTGC TTAAGGAGGC AGAAAGTTGT
    CTTGCAGATC TAAAAGCAGA TTCTGAATGG GAAGAATGTG AAACAGACTG CAGTGATGAT
    GAAATGAAGG GAGAGAACTC AGAATCAAGA AAACTAGAAG AAGAATTGGG AAGCCTCAAA
    GCTCAAGAAG AGGAACTTCA AAGAGAACTG TCAGATCTGG AGACTGAGAA TGAACAAATA
    CTTGCTCAGA TGAAGCATCT ACAGGAAGAT GAAAAGAGCT GCCAGGAACT CCTGGAGAGA
    TGCAACTTCA CTGAGTGGGA GATTAGTGAA TGGAGTGAAC TCCAGGCAGT CTTTAACTTT
    CTTCATGATT CTATTGAACT CACAGTTGTG TTTGGAACCC CAATAGATGG TGATGTTTTT
    GGTGAAAATC CTTCCAGAAA AATAGTTAGC CTGAGCTTTG AATCTCTCTT GGATGAGGAA
    AAAGCTCCAC TCTCCTCACG TTTAGTCCAA AGACTCATAT TCCAGTTTAT TGAAAGTCAG
    GGATGCTGGC AGGAAAAGTG TCCCACACTA CAATACTTGC CTCAGGTGCT TCACGATGTC
    TCTTTGGTGG TGAGTCGTTG TAGGATCTTG GGAGAGGAGA TCGAATTTCT GGAGAGATGG
    GGTGGAAAAT ACAATCTTCT CAAGACAGAT ATAATTGACA CAAATGTGAA GCTCCTCTTC
    TCCACTTCCA CCGCTTTTGC AAAGTTTGAG TTGACACTGT CTTTATCATC CAACTACCCA
    TCTGCTTCAC TGCCTTTCAC TGTTGAAAAA AAAATTGGGA ATATTGGACA TGAGGAAATT
    TCTGCTGTTC TCTCTAAAGT ACCTATTGGT TACCATTATC TCCGAAGAAT GGTGAGCTTG
    ATTCATCAAA ATCTGCTCCA GGACCCCAGA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa08803
    Clone ID Related Accession (Same CDS sequence) XM_420938.5
    Accession Version XM_420938.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7329bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product kinetochore scaffold 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_420938.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGCTGTACA GCGCTGCCGC CAGGAAGGCG GTTTGGCGCG GCGCGCAGGC GCGGCGGTTA 
    GGTTTGAATT GTGAGGCGGC AGCGGCGGCC CAGTCCTATG GGTGCTGTGG GGCTGGGGGT
    CAGCGGCGGA GAGCGGCCGA GAGTGTACTG TTTTTTATAG GAGAAACACT TCTTGCAGAA
    ATGGACAAAA TTTACATGGA CTCTAACGTG GAGAACGATA ACACAGAGCA CATCAGAGGA
    AAACGACTGT CCTCTATATT AAAGGCTCCA CGAAATCCTC TTGATGATCT GGGAAATGGG
    AATGAACTCA CCCAGGATCT CAACATAGAA AAACGTAGGA AAAGCTCTCG ACGTGTCAGC
    TTTGCCAACA CCATAAACTG CAGGGTCTTC CATCGGGATC TTAAGAATAA TGCAGCAGAA
    AGAGAAAATG AAGCAGAAGA TACGGAGAAT CATGTCCTGC TTAATCAAAA TGAAGAACCT
    GAAGCTGTTC CATGTGAGAT TACTGGGATG AACACCTTGC TTCATGCCCC TATTCAGTCA
    TTGGTGCAGC AGACTGATGA GTGCCATGAT GTGGACAATA CTATTCCGAG AACAGATAGG
    CCTGATGCAA CAGTCATCTT CTCAGATGAA AATGAAATGG ATATGACAGC TAGTCACACT
    GCAGTGATTG CACGTAACCT GAATAACAGT GAAACTGACA AAACTGAGAA AATAGATATT
    GCGTCATTTC TGGCTGAGTT AAATTCTATC AATGGAAAAG CAGAAAAAAA CAAAGATTTT
    CATTTTTCCT TTGATGCTGC AGACCGTTTT TGCCCATCTT TTGAACAGAA GGAAGATGCT
    ACTACAGGAA AGAAAATAAA TTCCAATGAA TTTTTCATGA ACTCAAATGA AAAAGCACTT
    AATCCTATTG AAGGACCTGA AAAAGAGAAC CTTTTTTTTG TCCCTTCAGA AGTGTCAGAA
    AACATGGCAC GATCATCAGT GGAATATGTA TATTCCCATG AACAACTAGC CACCTGCAAT
    GTAACAAAAA TATTTAGAGA GCAAGATGAT GGAATGGAAA TAACAAAGTG CCAAGCATCT
    GATATTCAAA ATATGCATTC TGGTCTTTCT GAGGTTCCGA CAGAGCAGTT AGCATCTGCA
    GATGTTACTC AGGCATTTGC AGATGATAGA ATGGATATGA CAACTAATCA CACTGCAAAA
    ATACATTTTC CCTTTGCCAT TGTAGGTAAT CAAAGTTTAA ATTCCAGAAA GGATTTCTCA
    TCCATGGAGC TAGATAACTA CTGTATTGTA AAGAGGACAT CTCACCAGCA ATTAATCCTA
    AAGCAAGACC CTCAACTTTG TACACGCAAA AAAATCATAA ATGTTGAAGA CACAGTAGAT
    GCTACAGCAC TGCAGGCTTT TAAGCAGGAG AGCAGAACAT TGTCTTCTAT CCCAGGATCT
    GTTTCTTCTG AGACTGTCTT CCGAGATGAT AAAACTGTTG TTTTTTCTCA ATGTGATGAC
    ATGGAAATTA CTGGGAATTA CAGAGATGTA CTCTATAATA CAAGTACCAG AGGAATGAAC
    AGTTCATGTA ATAAAGCTTT TGAAAAAACA GTTAACAGAA GTTCATTGCT AGTAGAAAGA
    CGACATCCGG TAAATAATGA CAATACAAGA AGTCTGTCTT TAGATGATAG ATCTTCTCCG
    TCACGCAAGA ATAGTGCACG TGAACTATCT TCAGGTTCAG ATGGACAAAA TGAAAGAGTA
    ACCCAAGATC ACAGTACTGT TTCATTGAAC ACTGGTTTCA ATTCATGTAC AAATTCAATG
    TCTACTGGTT CCTCTAAGGG TAGGCTGCAG CACAGACTAG CAAACTCCCT ACTTGTTTCA
    CTACCAGGTG AGAAAACTGC AATATTTTCA GGAACAGATG TGGACTTGAC TAAAAATTGT
    GTTGGCAAGG GTTATAGAAA GAATATTGAA AATGAATTTC CTCCTGGATT GTCCAACTCT
    GTCACTTGCA AACCACATTT TCTTGATAGT AGGTCTGTAT CTTCCAATTT AAGGGAGCAG
    GAAAAAATGG AAATAACTAA ATGTCAGACA GTTGTCAGTG GTGATCAAAG CATTGGGATA
    ACCACAGAAG CAGAACAAAG GCATTGTAAA ATAATTCCAA GTAAGAACCA GAACAATAAT
    GTTAGTGATT CTTTGGATAG GGACAAGGAA AATCTTGAGG CAATTGGTTT TGATGTGAGT
    ATTAGAAGAA GCCAGGCTAT AGAAGCGAAT GCTAAAGGTG TTGAGGTGGT AACTGTCAAT
    AAGAAGCCTG GAAAAACAGA CTTTCAGGCT AGTAGTCTGA GTTCCTGTGC AAAGAGCAGG
    TATTCACTCC AGGAGCAAAA GAGGGAAGTC TCTGAAAATA TTGTCACTGA CAAATGTGCA
    TTCACATCTT TGCAAAGTGA AAAAGTATAT GTATCCCAGC CTTTGGGAAA TAAACTTCCA
    AATTCTTCAG TTTCCTGCAC AAATGACAAA AGAGACATGT TTTCAGATGA TGAAAACATG
    GACATAACCA AAGCTCATTC TTTGGTTGCT CCTTCTATGA ATATAGTACA AGAAAATGAG
    AATACTCACG TTCAAAATAA TGTAAAAGCC AAACAGCTGC AAAACCAGAC CTTCCAGTTT
    TCTGATAATT CTGGTATAGA TCATACAAGT CAGACTGCAG CAATAGAATG TGGAGATGTC
    AAAAGGAGCT CAAATAAACA AACTGTTGCT CATTTAGCTC CAAGTGGTTT CTTTATTTCC
    AGTAATGAGC TTGCTCCCTC TGGAAAGAAA AGAGATGCAC AACCTGGAAA TACACTGGGA
    ATAATCTCAG ATTGCCAGAA CTCTGATAGG CATAAGAAAA CACATCCTAG GATAGTAGTA
    AACAATGTAT CATCAACTGC AGAAGACCGT GAGAGTGATT TTTTGATCAG TAAAACAGTT
    TCTTTAGAAG GGGGTTTTAA TAACCCTCAG TCAGGTGATG ACCTGTGCTT GAGGGGTGTT
    GAAGAGAGGT CACTAGCCAG TAATTCTAAA CAACTTAAGG GAAGTTCCCA TCTGCCTTCC
    TTTTTAGAAA AGTCAGTTGT ATTTTCCTCT GGTGAAAACA TGGACTTGAC TGAAAACTCT
    GCAGTAGTGG CTCCTGATCA AAACATCTGT ACTATTTTCC CAGGAAGAAA AGCAGCACCA
    GGACGTATTG AAGATAAAAG TAAAATATCC TTTAAAAAAG AGGTAATGAT GACAAACAGT
    CAGGAACAGC CTGGATGTGA TATGTACTCC TTGGTATCTG ATAAGAAAAT GTTAACTGTG
    ACTGGTATAA AGCATTTGCT TTCTGCAGAT GAGAAAACTA CCATCTTTTC AGAAGGTGCT
    AATATGGACA TCACTGGAAA TCATACAGCT TCAGCAGACA ACAAAACGAT TTTGCAGCCT
    GAAATTTTGA ACAATGACAT ATCCTTCATT TCTGAAGATA AAACTTCTGT TTTTACAAAA
    AATAACGACA TGGAAATAAG TAGGCTTGAT TCTGTTGCAA CTGATAATTC TATGGAAAAG
    GCTGTATCTC AAGGGATGCT TAGCAGAACT AATGGAGCTA GAAGGAAAAG CTTGAAGGGA
    ACAATAGGGG AAAAGACTGT TTTATTTTCA CTGAGTGAGA ATAATGACAT GAGCATCACA
    GAGGGACTTA CAGCAGCAAT AAGTCATGAA ATTGTCTCAC AAAATGAGGC AGGGGCTCCT
    TCTCCATCTT CAGCTCATCC TGTTACAAAT GTTACATTCA CATGTAACCA GGCTGACATT
    AAGTCTTGCT CTGCCAATAA AATTACTGTA AAAGTTCCAT GTGATGATGC GTTGATCTCA
    GATAAAGTTT TTGGACAGGA ATTTCCTTCA AACAGAGAAA CTACTGTGTT TGCTATGGAT
    GATATGGAAA TCACTAAGGC CCATAATGCA TCTTTAAAAG AAAATTTTCT GCAAAGTAAG
    CAACCCAATC AATCCGTTCC TTTAACTTCC TCAATTATAT TTACAAGTGA CCAGGCTGAC
    ATGGAAATGA CTGAGTATCA CCCTGTTGAT GAAAGTGTTG AAAGGGATTT ATGTGAGGAT
    ACATTAAAGC TGCCCGCAGA GGTTCTACAG GAGGCTCTTT CAGGTAGCAA AACAGTCATT
    TTTCCTTTGG ATGAGAATAT GGACGTCACA AAAACTCATG ATGTATTAAG TGGTAAGATC
    AGTGTATGGA ATGGAAGGTC CATTCCAGCC ATTTCTGCAG TTCCTGCTGA TAAGACCATT
    GTATTTCATC ACAACCAAGA TGATATGGAA ATAACTTCAT CACATACTGT TTCAGTTAAT
    AACAACACTG AAGGATCTGA GAATCAAAAA GCATCACATA AAAGCACTCA ACAACCAGAC
    TTGCGTAGCG TATCATCATC CTCTTGCAGA GATGAAATAG ATTCTCTACA AATAAAAGAC
    CTGAATGGTG GGGATCATAA AAAATCTAAT ATTAAGTCCT CAATTCCTTC TGCTTCATCG
    GCCTCAGCGT CACCAACTGA GAAAGGACCT ATCAAAGTCC ACAGTGGCAC AATCCCAGAT
    TCCATTTATT CTATCTCAAT TCCAAAAGAT GCTTTGGATG TCCAAGCACT ACAGGATCTT
    GGCCTTTCCA CAAAAAATTC AATTCCTATA AACAACGAGC AGGATCTTTT ACATCCAGGA
    GCAATGAAAT CAAGGAGAGC ATCTTTCAAG ATGCCAGGTA ATGACCCCTT GGACTGCAGT
    GAAGAAAGTG GAGTTGTAGT ATCCCATGTT TTTCTTCCTG CTCAGCACTC AGACTCTTTG
    AAGGATCCTC CAGATGCACT TAGTACTCAG AGAAATCAAG TATTGAAAGA TGATTCATCT
    AGACCTGAAG ATCTAGCCAC AGATTTAGCC ATGGATGGAG CAGGCATTGT TCCAGTTTCT
    AACAAGGAAT CGGAGGAAAA TGTGCTGTCA GCTGAAGGAG AGGCAACAGA ATTCCAAATA
    AACTCTGAAC AAACTAAGCA ACTTTCAGTC AGCGATAGTC TGAGAGATCC AATAGATCTT
    GCGCCTAAAT TATCAGGTAT TATAAATATT TGTTCAAAAC TGGAAAATAT TAGAAGGAAA
    TCTGTAGTTG TTTCTGAAAC TGTTCTTTCT GACCACTTGC TGAAGTTACC AACTCAGCCA
    GAGGATACCT TGATGTTAGG AAGAAATAGC ATAAATGAAC AAAGGTTATG TTATGCCAAA
    GAGCAGGAGA ATGCATGTCT TGAATCTGGA GCTGCTCCCA TAGATGCAAA TTTAGGAATG
    GCACTTAAGG ACAAATATAA AGGAAGAAAC TTCCCTCTAG GTATCTTTCA GCCTAAACTA
    CCAAACAGAA GAAATCCTTC TGTTTCTAGT GTGCAAGACA CAACTGCAAA ATCTTCAGGC
    AAAGTAGAAT CAGCAGTTCC AGAAGTAAAT CTGAACACCA TTAAAGCTCC AGGTTACAAC
    AACGCTAGTA GGCAGAACTT CAGCCCTTCT CAGTTTATAG CAGAAGAATT TCTTCCTGTC
    TGCCCAGAAG AAATGGATTC AAATGATTCT GTAAGCTCTG AGTTTGTGGA AGAGGCTTGC
    AATAACGGAA ACAAAAGAGA AATCCTCCAC AATGAAGAGA TTCAAGTTGA AGAGACAAAA
    ACTTGCAACA ATGCAAAAAG AGCTTTGAAA TGTGTTGAGG AGGATCTTCA AAGTCTAAAG
    AGGCTCAAGA TGGATGAAAT CGTGGTTGAT GGGACCTCCC AGGACTTGCA GGTTACTTAC
    GGCACTGTAC CTGAAAGCCA AGCAGAAGTT GCTGAAAGTG AAGATTCTCC AAATCTATCA
    GCTAAAAACC CTGACTGTAC ACATGCCAAC AGCAGCAGCT CTCTAGATTC TGTTAAGGCT
    GACACAGAAT TAACAATTCA ACGAAGCAGT CAAATGGAAT CGCAGCTTCT CACAGATAGC
    ATTTGTGAAG ATAATTTGTG GGAGAAATTT CAGAGTGGTG TCATCACAGT TGCGGAGTTT
    TTTACTCTTC TTCAAGTCCA TATTCCAATT CAGAAGCCCC GGCAGAGCCA TATTCCAGCC
    AACTGTGCTG TCAGTGCTCC ACCTACTCCA GAAGACCTGA TTCTCAGCCA ATACGTTTAT
    CGTCCCAAGA TGCGTATTTA TGAAGAAGAC TGCCAGGTTC TTTCTCAGAT GATAGATGAA
    TTAAAACAGT ATGTAAGTGT CCAGGATCAA CCACTGGTGA ATGTGAATAA GAGTTTGTGG
    GAAGTAATGA GAACCTGCTC TGACGAAGAG CTGAAGAGCT TTGGAGCAGA ACTGAACAAA
    ATGAAATCCC ATTTCATCAA GGAAAGTAAA ATCCTGGCTC ACAATGAGAA AGAGGCATTA
    TACAGCAAAT TGCTGCAGAG TGCGCAGGAA CAATATGAAA AGCTCCAGTC AAGAACAGCA
    AACATGGATG AATTGCTTAA GGAGGCAGAA AGTTGTCTTG CAGATCTAAA AGCAGATTCT
    GAATGGGAAG AATGTGAAAC AGACTGCAGT GATGATGAAA TGAAGGGAGA GAACTCAGAA
    TCAAGAAAAC TAGAAGAAGA ATTGGGAAGC CTCAAAGCTC AAGAAGAGGA ACTTCAAAGA
    GAACTGTCAG ATCTGGAGAC TGAGAATGAA CAAATACTTG CTCAGATGAA GCATCTACAG
    GAAGATGAAA AGAGCTGCCA GGAACTCCTG GAGAGATGCA ACTTCACTGA GTGGGAGATT
    AGTGAATGGA GTGAACTCCA GGCAGTCTTT AACTTTCTTC ATGATTCTAT TGAACTCACA
    GTTGTGTTTG GAACCCCAAT AGATGGTGAT GTTTTTGGTG AAAATCCTTC CAGAAAAATA
    GTTAGCCTGA GCTTTGAATC TCTCTTGGAT GAGGAAAAAG CTCCACTCTC CTCACGTTTA
    GTCCAAAGAC TCATATTCCA GTTTATTGAA AGTCAGGGAT GCTGGCAGGA AAAGTGTCCC
    ACACTACAAT ACTTGCCTCA GGTGCTTCAC GATGTCTCTT TGGTGGTGAG TCGTTGTAGG
    ATCTTGGGAG AGGAGATCGA ATTTCTGGAG AGATGGGGTG GAAAATACAA TCTTCTCAAG
    ACAGATATAA TTGACACAAA TGTGAAGCTC CTCTTCTCCA CTTCCACCGC TTTTGCAAAG
    TTTGAGTTGA CACTGTCTTT ATCATCCAAC TACCCATCTG CTTCACTGCC TTTCACTGTT
    GAAAAAAAAA TTGGGAATAT TGGACATGAG GAAATTTCTG CTGTTCTCTC TAAAGTACCT
    ATTGGTTACC ATTATCTCCG AAGAATGGTG AGCTTGATTC ATCAAAATCT GCTCCAGGAC
    CCCAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_420938.4
    CDS16..7344
    Translation

    Target ORF information:

    RefSeq Version XM_420938.5
    Organism Gallus gallus(chicken)
    Definition Gallus gallus kinetochore scaffold 1 (KNL1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_420938.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGCTGTACA GCGCTGCCGC CAGGAAGGCG GTTTGGCGCG GCGCGCAGGC GCGGCGGTTA 
    GGTTTGAATT GTGAGGCGGC AGCGGCGGCC CAGTCCTATG GGTGCTGTGG GGCTGGGGGT
    CAGCGGCGGA GAGCGGCCGA GAGTGTACTG TTTTTTATAG GAGAAACACT TCTTGCAGAA
    ATGGACAAAA TTTACATGGA CTCTAACGTG GAGAACGATA ACACAGAGCA CATCAGAGGA
    AAACGACTGT CCTCTATATT AAAGGCTCCA CGAAATCCTC TTGATGATCT GGGAAATGGG
    AATGAACTCA CCCAGGATCT CAACATAGAA AAACGTAGGA AAAGCTCTCG ACGTGTCAGC
    TTTGCCAACA CCATAAACTG CAGGGTCTTC CATCGGGATC TTAAGAATAA TGCAGCAGAA
    AGAGAAAATG AAGCAGAAGA TACGGAGAAT CATGTCCTGC TTAATCAAAA TGAAGAACCT
    GAAGCTGTTC CATGTGAGAT TACTGGGATG AACACCTTGC TTCATGCCCC TATTCAGTCA
    TTGGTGCAGC AGACTGATGA GTGCCATGAT GTGGACAATA CTATTCCGAG AACAGATAGG
    CCTGATGCAA CAGTCATCTT CTCAGATGAA AATGAAATGG ATATGACAGC TAGTCACACT
    GCAGTGATTG CACGTAACCT GAATAACAGT GAAACTGACA AAACTGAGAA AATAGATATT
    GCGTCATTTC TGGCTGAGTT AAATTCTATC AATGGAAAAG CAGAAAAAAA CAAAGATTTT
    CATTTTTCCT TTGATGCTGC AGACCGTTTT TGCCCATCTT TTGAACAGAA GGAAGATGCT
    ACTACAGGAA AGAAAATAAA TTCCAATGAA TTTTTCATGA ACTCAAATGA AAAAGCACTT
    AATCCTATTG AAGGACCTGA AAAAGAGAAC CTTTTTTTTG TCCCTTCAGA AGTGTCAGAA
    AACATGGCAC GATCATCAGT GGAATATGTA TATTCCCATG AACAACTAGC CACCTGCAAT
    GTAACAAAAA TATTTAGAGA GCAAGATGAT GGAATGGAAA TAACAAAGTG CCAAGCATCT
    GATATTCAAA ATATGCATTC TGGTCTTTCT GAGGTTCCGA CAGAGCAGTT AGCATCTGCA
    GATGTTACTC AGGCATTTGC AGATGATAGA ATGGATATGA CAACTAATCA CACTGCAAAA
    ATACATTTTC CCTTTGCCAT TGTAGGTAAT CAAAGTTTAA ATTCCAGAAA GGATTTCTCA
    TCCATGGAGC TAGATAACTA CTGTATTGTA AAGAGGACAT CTCACCAGCA ATTAATCCTA
    AAGCAAGACC CTCAACTTTG TACACGCAAA AAAATCATAA ATGTTGAAGA CACAGTAGAT
    GCTACAGCAC TGCAGGCTTT TAAGCAGGAG AGCAGAACAT TGTCTTCTAT CCCAGGATCT
    GTTTCTTCTG AGACTGTCTT CCGAGATGAT AAAACTGTTG TTTTTTCTCA ATGTGATGAC
    ATGGAAATTA CTGGGAATTA CAGAGATGTA CTCTATAATA CAAGTACCAG AGGAATGAAC
    AGTTCATGTA ATAAAGCTTT TGAAAAAACA GTTAACAGAA GTTCATTGCT AGTAGAAAGA
    CGACATCCGG TAAATAATGA CAATACAAGA AGTCTGTCTT TAGATGATAG ATCTTCTCCG
    TCACGCAAGA ATAGTGCACG TGAACTATCT TCAGGTTCAG ATGGACAAAA TGAAAGAGTA
    ACCCAAGATC ACAGTACTGT TTCATTGAAC ACTGGTTTCA ATTCATGTAC AAATTCAATG
    TCTACTGGTT CCTCTAAGGG TAGGCTGCAG CACAGACTAG CAAACTCCCT ACTTGTTTCA
    CTACCAGGTG AGAAAACTGC AATATTTTCA GGAACAGATG TGGACTTGAC TAAAAATTGT
    GTTGGCAAGG GTTATAGAAA GAATATTGAA AATGAATTTC CTCCTGGATT GTCCAACTCT
    GTCACTTGCA AACCACATTT TCTTGATAGT AGGTCTGTAT CTTCCAATTT AAGGGAGCAG
    GAAAAAATGG AAATAACTAA ATGTCAGACA GTTGTCAGTG GTGATCAAAG CATTGGGATA
    ACCACAGAAG CAGAACAAAG GCATTGTAAA ATAATTCCAA GTAAGAACCA GAACAATAAT
    GTTAGTGATT CTTTGGATAG GGACAAGGAA AATCTTGAGG CAATTGGTTT TGATGTGAGT
    ATTAGAAGAA GCCAGGCTAT AGAAGCGAAT GCTAAAGGTG TTGAGGTGGT AACTGTCAAT
    AAGAAGCCTG GAAAAACAGA CTTTCAGGCT AGTAGTCTGA GTTCCTGTGC AAAGAGCAGG
    TATTCACTCC AGGAGCAAAA GAGGGAAGTC TCTGAAAATA TTGTCACTGA CAAATGTGCA
    TTCACATCTT TGCAAAGTGA AAAAGTATAT GTATCCCAGC CTTTGGGAAA TAAACTTCCA
    AATTCTTCAG TTTCCTGCAC AAATGACAAA AGAGACATGT TTTCAGATGA TGAAAACATG
    GACATAACCA AAGCTCATTC TTTGGTTGCT CCTTCTATGA ATATAGTACA AGAAAATGAG
    AATACTCACG TTCAAAATAA TGTAAAAGCC AAACAGCTGC AAAACCAGAC CTTCCAGTTT
    TCTGATAATT CTGGTATAGA TCATACAAGT CAGACTGCAG CAATAGAATG TGGAGATGTC
    AAAAGGAGCT CAAATAAACA AACTGTTGCT CATTTAGCTC CAAGTGGTTT CTTTATTTCC
    AGTAATGAGC TTGCTCCCTC TGGAAAGAAA AGAGATGCAC AACCTGGAAA TACACTGGGA
    ATAATCTCAG ATTGCCAGAA CTCTGATAGG CATAAGAAAA CACATCCTAG GATAGTAGTA
    AACAATGTAT CATCAACTGC AGAAGACCGT GAGAGTGATT TTTTGATCAG TAAAACAGTT
    TCTTTAGAAG GGGGTTTTAA TAACCCTCAG TCAGGTGATG ACCTGTGCTT GAGGGGTGTT
    GAAGAGAGGT CACTAGCCAG TAATTCTAAA CAACTTAAGG GAAGTTCCCA TCTGCCTTCC
    TTTTTAGAAA AGTCAGTTGT ATTTTCCTCT GGTGAAAACA TGGACTTGAC TGAAAACTCT
    GCAGTAGTGG CTCCTGATCA AAACATCTGT ACTATTTTCC CAGGAAGAAA AGCAGCACCA
    GGACGTATTG AAGATAAAAG TAAAATATCC TTTAAAAAAG AGGTAATGAT GACAAACAGT
    CAGGAACAGC CTGGATGTGA TATGTACTCC TTGGTATCTG ATAAGAAAAT GTTAACTGTG
    ACTGGTATAA AGCATTTGCT TTCTGCAGAT GAGAAAACTA CCATCTTTTC AGAAGGTGCT
    AATATGGACA TCACTGGAAA TCATACAGCT TCAGCAGACA ACAAAACGAT TTTGCAGCCT
    GAAATTTTGA ACAATGACAT ATCCTTCATT TCTGAAGATA AAACTTCTGT TTTTACAAAA
    AATAACGACA TGGAAATAAG TAGGCTTGAT TCTGTTGCAA CTGATAATTC TATGGAAAAG
    GCTGTATCTC AAGGGATGCT TAGCAGAACT AATGGAGCTA GAAGGAAAAG CTTGAAGGGA
    ACAATAGGGG AAAAGACTGT TTTATTTTCA CTGAGTGAGA ATAATGACAT GAGCATCACA
    GAGGGACTTA CAGCAGCAAT AAGTCATGAA ATTGTCTCAC AAAATGAGGC AGGGGCTCCT
    TCTCCATCTT CAGCTCATCC TGTTACAAAT GTTACATTCA CATGTAACCA GGCTGACATT
    AAGTCTTGCT CTGCCAATAA AATTACTGTA AAAGTTCCAT GTGATGATGC GTTGATCTCA
    GATAAAGTTT TTGGACAGGA ATTTCCTTCA AACAGAGAAA CTACTGTGTT TGCTATGGAT
    GATATGGAAA TCACTAAGGC CCATAATGCA TCTTTAAAAG AAAATTTTCT GCAAAGTAAG
    CAACCCAATC AATCCGTTCC TTTAACTTCC TCAATTATAT TTACAAGTGA CCAGGCTGAC
    ATGGAAATGA CTGAGTATCA CCCTGTTGAT GAAAGTGTTG AAAGGGATTT ATGTGAGGAT
    ACATTAAAGC TGCCCGCAGA GGTTCTACAG GAGGCTCTTT CAGGTAGCAA AACAGTCATT
    TTTCCTTTGG ATGAGAATAT GGACGTCACA AAAACTCATG ATGTATTAAG TGGTAAGATC
    AGTGTATGGA ATGGAAGGTC CATTCCAGCC ATTTCTGCAG TTCCTGCTGA TAAGACCATT
    GTATTTCATC ACAACCAAGA TGATATGGAA ATAACTTCAT CACATACTGT TTCAGTTAAT
    AACAACACTG AAGGATCTGA GAATCAAAAA GCATCACATA AAAGCACTCA ACAACCAGAC
    TTGCGTAGCG TATCATCATC CTCTTGCAGA GATGAAATAG ATTCTCTACA AATAAAAGAC
    CTGAATGGTG GGGATCATAA AAAATCTAAT ATTAAGTCCT CAATTCCTTC TGCTTCATCG
    GCCTCAGCGT CACCAACTGA GAAAGGACCT ATCAAAGTCC ACAGTGGCAC AATCCCAGAT
    TCCATTTATT CTATCTCAAT TCCAAAAGAT GCTTTGGATG TCCAAGCACT ACAGGATCTT
    GGCCTTTCCA CAAAAAATTC AATTCCTATA AACAACGAGC AGGATCTTTT ACATCCAGGA
    GCAATGAAAT CAAGGAGAGC ATCTTTCAAG ATGCCAGGTA ATGACCCCTT GGACTGCAGT
    GAAGAAAGTG GAGTTGTAGT ATCCCATGTT TTTCTTCCTG CTCAGCACTC AGACTCTTTG
    AAGGATCCTC CAGATGCACT TAGTACTCAG AGAAATCAAG TATTGAAAGA TGATTCATCT
    AGACCTGAAG ATCTAGCCAC AGATTTAGCC ATGGATGGAG CAGGCATTGT TCCAGTTTCT
    AACAAGGAAT CGGAGGAAAA TGTGCTGTCA GCTGAAGGAG AGGCAACAGA ATTCCAAATA
    AACTCTGAAC AAACTAAGCA ACTTTCAGTC AGCGATAGTC TGAGAGATCC AATAGATCTT
    GCGCCTAAAT TATCAGGTAT TATAAATATT TGTTCAAAAC TGGAAAATAT TAGAAGGAAA
    TCTGTAGTTG TTTCTGAAAC TGTTCTTTCT GACCACTTGC TGAAGTTACC AACTCAGCCA
    GAGGATACCT TGATGTTAGG AAGAAATAGC ATAAATGAAC AAAGGTTATG TTATGCCAAA
    GAGCAGGAGA ATGCATGTCT TGAATCTGGA GCTGCTCCCA TAGATGCAAA TTTAGGAATG
    GCACTTAAGG ACAAATATAA AGGAAGAAAC TTCCCTCTAG GTATCTTTCA GCCTAAACTA
    CCAAACAGAA GAAATCCTTC TGTTTCTAGT GTGCAAGACA CAACTGCAAA ATCTTCAGGC
    AAAGTAGAAT CAGCAGTTCC AGAAGTAAAT CTGAACACCA TTAAAGCTCC AGGTTACAAC
    AACGCTAGTA GGCAGAACTT CAGCCCTTCT CAGTTTATAG CAGAAGAATT TCTTCCTGTC
    TGCCCAGAAG AAATGGATTC AAATGATTCT GTAAGCTCTG AGTTTGTGGA AGAGGCTTGC
    AATAACGGAA ACAAAAGAGA AATCCTCCAC AATGAAGAGA TTCAAGTTGA AGAGACAAAA
    ACTTGCAACA ATGCAAAAAG AGCTTTGAAA TGTGTTGAGG AGGATCTTCA AAGTCTAAAG
    AGGCTCAAGA TGGATGAAAT CGTGGTTGAT GGGACCTCCC AGGACTTGCA GGTTACTTAC
    GGCACTGTAC CTGAAAGCCA AGCAGAAGTT GCTGAAAGTG AAGATTCTCC AAATCTATCA
    GCTAAAAACC CTGACTGTAC ACATGCCAAC AGCAGCAGCT CTCTAGATTC TGTTAAGGCT
    GACACAGAAT TAACAATTCA ACGAAGCAGT CAAATGGAAT CGCAGCTTCT CACAGATAGC
    ATTTGTGAAG ATAATTTGTG GGAGAAATTT CAGAGTGGTG TCATCACAGT TGCGGAGTTT
    TTTACTCTTC TTCAAGTCCA TATTCCAATT CAGAAGCCCC GGCAGAGCCA TATTCCAGCC
    AACTGTGCTG TCAGTGCTCC ACCTACTCCA GAAGACCTGA TTCTCAGCCA ATACGTTTAT
    CGTCCCAAGA TGCGTATTTA TGAAGAAGAC TGCCAGGTTC TTTCTCAGAT GATAGATGAA
    TTAAAACAGT ATGTAAGTGT CCAGGATCAA CCACTGGTGA ATGTGAATAA GAGTTTGTGG
    GAAGTAATGA GAACCTGCTC TGACGAAGAG CTGAAGAGCT TTGGAGCAGA ACTGAACAAA
    ATGAAATCCC ATTTCATCAA GGAAAGTAAA ATCCTGGCTC ACAATGAGAA AGAGGCATTA
    TACAGCAAAT TGCTGCAGAG TGCGCAGGAA CAATATGAAA AGCTCCAGTC AAGAACAGCA
    AACATGGATG AATTGCTTAA GGAGGCAGAA AGTTGTCTTG CAGATCTAAA AGCAGATTCT
    GAATGGGAAG AATGTGAAAC AGACTGCAGT GATGATGAAA TGAAGGGAGA GAACTCAGAA
    TCAAGAAAAC TAGAAGAAGA ATTGGGAAGC CTCAAAGCTC AAGAAGAGGA ACTTCAAAGA
    GAACTGTCAG ATCTGGAGAC TGAGAATGAA CAAATACTTG CTCAGATGAA GCATCTACAG
    GAAGATGAAA AGAGCTGCCA GGAACTCCTG GAGAGATGCA ACTTCACTGA GTGGGAGATT
    AGTGAATGGA GTGAACTCCA GGCAGTCTTT AACTTTCTTC ATGATTCTAT TGAACTCACA
    GTTGTGTTTG GAACCCCAAT AGATGGTGAT GTTTTTGGTG AAAATCCTTC CAGAAAAATA
    GTTAGCCTGA GCTTTGAATC TCTCTTGGAT GAGGAAAAAG CTCCACTCTC CTCACGTTTA
    GTCCAAAGAC TCATATTCCA GTTTATTGAA AGTCAGGGAT GCTGGCAGGA AAAGTGTCCC
    ACACTACAAT ACTTGCCTCA GGTGCTTCAC GATGTCTCTT TGGTGGTGAG TCGTTGTAGG
    ATCTTGGGAG AGGAGATCGA ATTTCTGGAG AGATGGGGTG GAAAATACAA TCTTCTCAAG
    ACAGATATAA TTGACACAAA TGTGAAGCTC CTCTTCTCCA CTTCCACCGC TTTTGCAAAG
    TTTGAGTTGA CACTGTCTTT ATCATCCAAC TACCCATCTG CTTCACTGCC TTTCACTGTT
    GAAAAAAAAA TTGGGAATAT TGGACATGAG GAAATTTCTG CTGTTCTCTC TAAAGTACCT
    ATTGGTTACC ATTATCTCCG AAGAATGGTG AGCTTGATTC ATCAAAATCT GCTCCAGGAC
    CCCAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa08804
    Clone ID Related Accession (Same CDS sequence) XM_004941298.2
    Accession Version XM_004941298.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7326bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product kinetochore scaffold 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004941298.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGCTGTACA GCGCTGCCGC CAGGAAGGCG GTTTGGCGCG GCGCGCAGGC GCGGCGGTTA 
    GGTTTGAATT GTGAGGCGGC AGCGGCGGCC CAGTCCTATG GGTGCTGTGG GGCTGGGGGT
    CAGCGGCGGA GAGCGGCCGA GAGTGTACTG TTTTTTATAG GAGAAACACT TCTTGCAGAA
    ATGGACAAAA TTTACATGGA CTCTAACGTG GAGAACGATA ACACAGAGCA CATCAGAGGA
    AAACGACTGT CCTCTATATT AAAGGCTCCA CGAAATCCTC TTGATGATCT GGGAAATGGG
    AATGAACTCA CCCAGGATCT CAACATAGAA AAACGTAGGA AAAGCTCTCG ACGTGTCAGC
    TTTGCCAACA CCATAAACTG CAGGGTCTTC CATCGGGATC TTAAGAATAA TGCAGCAGAA
    AGAGAAAATG AAGAAGATAC GGAGAATCAT GTCCTGCTTA ATCAAAATGA AGAACCTGAA
    GCTGTTCCAT GTGAGATTAC TGGGATGAAC ACCTTGCTTC ATGCCCCTAT TCAGTCATTG
    GTGCAGCAGA CTGATGAGTG CCATGATGTG GACAATACTA TTCCGAGAAC AGATAGGCCT
    GATGCAACAG TCATCTTCTC AGATGAAAAT GAAATGGATA TGACAGCTAG TCACACTGCA
    GTGATTGCAC GTAACCTGAA TAACAGTGAA ACTGACAAAA CTGAGAAAAT AGATATTGCG
    TCATTTCTGG CTGAGTTAAA TTCTATCAAT GGAAAAGCAG AAAAAAACAA AGATTTTCAT
    TTTTCCTTTG ATGCTGCAGA CCGTTTTTGC CCATCTTTTG AACAGAAGGA AGATGCTACT
    ACAGGAAAGA AAATAAATTC CAATGAATTT TTCATGAACT CAAATGAAAA AGCACTTAAT
    CCTATTGAAG GACCTGAAAA AGAGAACCTT TTTTTTGTCC CTTCAGAAGT GTCAGAAAAC
    ATGGCACGAT CATCAGTGGA ATATGTATAT TCCCATGAAC AACTAGCCAC CTGCAATGTA
    ACAAAAATAT TTAGAGAGCA AGATGATGGA ATGGAAATAA CAAAGTGCCA AGCATCTGAT
    ATTCAAAATA TGCATTCTGG TCTTTCTGAG GTTCCGACAG AGCAGTTAGC ATCTGCAGAT
    GTTACTCAGG CATTTGCAGA TGATAGAATG GATATGACAA CTAATCACAC TGCAAAAATA
    CATTTTCCCT TTGCCATTGT AGGTAATCAA AGTTTAAATT CCAGAAAGGA TTTCTCATCC
    ATGGAGCTAG ATAACTACTG TATTGTAAAG AGGACATCTC ACCAGCAATT AATCCTAAAG
    CAAGACCCTC AACTTTGTAC ACGCAAAAAA ATCATAAATG TTGAAGACAC AGTAGATGCT
    ACAGCACTGC AGGCTTTTAA GCAGGAGAGC AGAACATTGT CTTCTATCCC AGGATCTGTT
    TCTTCTGAGA CTGTCTTCCG AGATGATAAA ACTGTTGTTT TTTCTCAATG TGATGACATG
    GAAATTACTG GGAATTACAG AGATGTACTC TATAATACAA GTACCAGAGG AATGAACAGT
    TCATGTAATA AAGCTTTTGA AAAAACAGTT AACAGAAGTT CATTGCTAGT AGAAAGACGA
    CATCCGGTAA ATAATGACAA TACAAGAAGT CTGTCTTTAG ATGATAGATC TTCTCCGTCA
    CGCAAGAATA GTGCACGTGA ACTATCTTCA GGTTCAGATG GACAAAATGA AAGAGTAACC
    CAAGATCACA GTACTGTTTC ATTGAACACT GGTTTCAATT CATGTACAAA TTCAATGTCT
    ACTGGTTCCT CTAAGGGTAG GCTGCAGCAC AGACTAGCAA ACTCCCTACT TGTTTCACTA
    CCAGGTGAGA AAACTGCAAT ATTTTCAGGA ACAGATGTGG ACTTGACTAA AAATTGTGTT
    GGCAAGGGTT ATAGAAAGAA TATTGAAAAT GAATTTCCTC CTGGATTGTC CAACTCTGTC
    ACTTGCAAAC CACATTTTCT TGATAGTAGG TCTGTATCTT CCAATTTAAG GGAGCAGGAA
    AAAATGGAAA TAACTAAATG TCAGACAGTT GTCAGTGGTG ATCAAAGCAT TGGGATAACC
    ACAGAAGCAG AACAAAGGCA TTGTAAAATA ATTCCAAGTA AGAACCAGAA CAATAATGTT
    AGTGATTCTT TGGATAGGGA CAAGGAAAAT CTTGAGGCAA TTGGTTTTGA TGTGAGTATT
    AGAAGAAGCC AGGCTATAGA AGCGAATGCT AAAGGTGTTG AGGTGGTAAC TGTCAATAAG
    AAGCCTGGAA AAACAGACTT TCAGGCTAGT AGTCTGAGTT CCTGTGCAAA GAGCAGGTAT
    TCACTCCAGG AGCAAAAGAG GGAAGTCTCT GAAAATATTG TCACTGACAA ATGTGCATTC
    ACATCTTTGC AAAGTGAAAA AGTATATGTA TCCCAGCCTT TGGGAAATAA ACTTCCAAAT
    TCTTCAGTTT CCTGCACAAA TGACAAAAGA GACATGTTTT CAGATGATGA AAACATGGAC
    ATAACCAAAG CTCATTCTTT GGTTGCTCCT TCTATGAATA TAGTACAAGA AAATGAGAAT
    ACTCACGTTC AAAATAATGT AAAAGCCAAA CAGCTGCAAA ACCAGACCTT CCAGTTTTCT
    GATAATTCTG GTATAGATCA TACAAGTCAG ACTGCAGCAA TAGAATGTGG AGATGTCAAA
    AGGAGCTCAA ATAAACAAAC TGTTGCTCAT TTAGCTCCAA GTGGTTTCTT TATTTCCAGT
    AATGAGCTTG CTCCCTCTGG AAAGAAAAGA GATGCACAAC CTGGAAATAC ACTGGGAATA
    ATCTCAGATT GCCAGAACTC TGATAGGCAT AAGAAAACAC ATCCTAGGAT AGTAGTAAAC
    AATGTATCAT CAACTGCAGA AGACCGTGAG AGTGATTTTT TGATCAGTAA AACAGTTTCT
    TTAGAAGGGG GTTTTAATAA CCCTCAGTCA GGTGATGACC TGTGCTTGAG GGGTGTTGAA
    GAGAGGTCAC TAGCCAGTAA TTCTAAACAA CTTAAGGGAA GTTCCCATCT GCCTTCCTTT
    TTAGAAAAGT CAGTTGTATT TTCCTCTGGT GAAAACATGG ACTTGACTGA AAACTCTGCA
    GTAGTGGCTC CTGATCAAAA CATCTGTACT ATTTTCCCAG GAAGAAAAGC AGCACCAGGA
    CGTATTGAAG ATAAAAGTAA AATATCCTTT AAAAAAGAGG TAATGATGAC AAACAGTCAG
    GAACAGCCTG GATGTGATAT GTACTCCTTG GTATCTGATA AGAAAATGTT AACTGTGACT
    GGTATAAAGC ATTTGCTTTC TGCAGATGAG AAAACTACCA TCTTTTCAGA AGGTGCTAAT
    ATGGACATCA CTGGAAATCA TACAGCTTCA GCAGACAACA AAACGATTTT GCAGCCTGAA
    ATTTTGAACA ATGACATATC CTTCATTTCT GAAGATAAAA CTTCTGTTTT TACAAAAAAT
    AACGACATGG AAATAAGTAG GCTTGATTCT GTTGCAACTG ATAATTCTAT GGAAAAGGCT
    GTATCTCAAG GGATGCTTAG CAGAACTAAT GGAGCTAGAA GGAAAAGCTT GAAGGGAACA
    ATAGGGGAAA AGACTGTTTT ATTTTCACTG AGTGAGAATA ATGACATGAG CATCACAGAG
    GGACTTACAG CAGCAATAAG TCATGAAATT GTCTCACAAA ATGAGGCAGG GGCTCCTTCT
    CCATCTTCAG CTCATCCTGT TACAAATGTT ACATTCACAT GTAACCAGGC TGACATTAAG
    TCTTGCTCTG CCAATAAAAT TACTGTAAAA GTTCCATGTG ATGATGCGTT GATCTCAGAT
    AAAGTTTTTG GACAGGAATT TCCTTCAAAC AGAGAAACTA CTGTGTTTGC TATGGATGAT
    ATGGAAATCA CTAAGGCCCA TAATGCATCT TTAAAAGAAA ATTTTCTGCA AAGTAAGCAA
    CCCAATCAAT CCGTTCCTTT AACTTCCTCA ATTATATTTA CAAGTGACCA GGCTGACATG
    GAAATGACTG AGTATCACCC TGTTGATGAA AGTGTTGAAA GGGATTTATG TGAGGATACA
    TTAAAGCTGC CCGCAGAGGT TCTACAGGAG GCTCTTTCAG GTAGCAAAAC AGTCATTTTT
    CCTTTGGATG AGAATATGGA CGTCACAAAA ACTCATGATG TATTAAGTGG TAAGATCAGT
    GTATGGAATG GAAGGTCCAT TCCAGCCATT TCTGCAGTTC CTGCTGATAA GACCATTGTA
    TTTCATCACA ACCAAGATGA TATGGAAATA ACTTCATCAC ATACTGTTTC AGTTAATAAC
    AACACTGAAG GATCTGAGAA TCAAAAAGCA TCACATAAAA GCACTCAACA ACCAGACTTG
    CGTAGCGTAT CATCATCCTC TTGCAGAGAT GAAATAGATT CTCTACAAAT AAAAGACCTG
    AATGGTGGGG ATCATAAAAA ATCTAATATT AAGTCCTCAA TTCCTTCTGC TTCATCGGCC
    TCAGCGTCAC CAACTGAGAA AGGACCTATC AAAGTCCACA GTGGCACAAT CCCAGATTCC
    ATTTATTCTA TCTCAATTCC AAAAGATGCT TTGGATGTCC AAGCACTACA GGATCTTGGC
    CTTTCCACAA AAAATTCAAT TCCTATAAAC AACGAGCAGG ATCTTTTACA TCCAGGAGCA
    ATGAAATCAA GGAGAGCATC TTTCAAGATG CCAGGTAATG ACCCCTTGGA CTGCAGTGAA
    GAAAGTGGAG TTGTAGTATC CCATGTTTTT CTTCCTGCTC AGCACTCAGA CTCTTTGAAG
    GATCCTCCAG ATGCACTTAG TACTCAGAGA AATCAAGTAT TGAAAGATGA TTCATCTAGA
    CCTGAAGATC TAGCCACAGA TTTAGCCATG GATGGAGCAG GCATTGTTCC AGTTTCTAAC
    AAGGAATCGG AGGAAAATGT GCTGTCAGCT GAAGGAGAGG CAACAGAATT CCAAATAAAC
    TCTGAACAAA CTAAGCAACT TTCAGTCAGC GATAGTCTGA GAGATCCAAT AGATCTTGCG
    CCTAAATTAT CAGGTATTAT AAATATTTGT TCAAAACTGG AAAATATTAG AAGGAAATCT
    GTAGTTGTTT CTGAAACTGT TCTTTCTGAC CACTTGCTGA AGTTACCAAC TCAGCCAGAG
    GATACCTTGA TGTTAGGAAG AAATAGCATA AATGAACAAA GGTTATGTTA TGCCAAAGAG
    CAGGAGAATG CATGTCTTGA ATCTGGAGCT GCTCCCATAG ATGCAAATTT AGGAATGGCA
    CTTAAGGACA AATATAAAGG AAGAAACTTC CCTCTAGGTA TCTTTCAGCC TAAACTACCA
    AACAGAAGAA ATCCTTCTGT TTCTAGTGTG CAAGACACAA CTGCAAAATC TTCAGGCAAA
    GTAGAATCAG CAGTTCCAGA AGTAAATCTG AACACCATTA AAGCTCCAGG TTACAACAAC
    GCTAGTAGGC AGAACTTCAG CCCTTCTCAG TTTATAGCAG AAGAATTTCT TCCTGTCTGC
    CCAGAAGAAA TGGATTCAAA TGATTCTGTA AGCTCTGAGT TTGTGGAAGA GGCTTGCAAT
    AACGGAAACA AAAGAGAAAT CCTCCACAAT GAAGAGATTC AAGTTGAAGA GACAAAAACT
    TGCAACAATG CAAAAAGAGC TTTGAAATGT GTTGAGGAGG ATCTTCAAAG TCTAAAGAGG
    CTCAAGATGG ATGAAATCGT GGTTGATGGG ACCTCCCAGG ACTTGCAGGT TACTTACGGC
    ACTGTACCTG AAAGCCAAGC AGAAGTTGCT GAAAGTGAAG ATTCTCCAAA TCTATCAGCT
    AAAAACCCTG ACTGTACACA TGCCAACAGC AGCAGCTCTC TAGATTCTGT TAAGGCTGAC
    ACAGAATTAA CAATTCAACG AAGCAGTCAA ATGGAATCGC AGCTTCTCAC AGATAGCATT
    TGTGAAGATA ATTTGTGGGA GAAATTTCAG AGTGGTGTCA TCACAGTTGC GGAGTTTTTT
    ACTCTTCTTC AAGTCCATAT TCCAATTCAG AAGCCCCGGC AGAGCCATAT TCCAGCCAAC
    TGTGCTGTCA GTGCTCCACC TACTCCAGAA GACCTGATTC TCAGCCAATA CGTTTATCGT
    CCCAAGATGC GTATTTATGA AGAAGACTGC CAGGTTCTTT CTCAGATGAT AGATGAATTA
    AAACAGTATG TAAGTGTCCA GGATCAACCA CTGGTGAATG TGAATAAGAG TTTGTGGGAA
    GTAATGAGAA CCTGCTCTGA CGAAGAGCTG AAGAGCTTTG GAGCAGAACT GAACAAAATG
    AAATCCCATT TCATCAAGGA AAGTAAAATC CTGGCTCACA ATGAGAAAGA GGCATTATAC
    AGCAAATTGC TGCAGAGTGC GCAGGAACAA TATGAAAAGC TCCAGTCAAG AACAGCAAAC
    ATGGATGAAT TGCTTAAGGA GGCAGAAAGT TGTCTTGCAG ATCTAAAAGC AGATTCTGAA
    TGGGAAGAAT GTGAAACAGA CTGCAGTGAT GATGAAATGA AGGGAGAGAA CTCAGAATCA
    AGAAAACTAG AAGAAGAATT GGGAAGCCTC AAAGCTCAAG AAGAGGAACT TCAAAGAGAA
    CTGTCAGATC TGGAGACTGA GAATGAACAA ATACTTGCTC AGATGAAGCA TCTACAGGAA
    GATGAAAAGA GCTGCCAGGA ACTCCTGGAG AGATGCAACT TCACTGAGTG GGAGATTAGT
    GAATGGAGTG AACTCCAGGC AGTCTTTAAC TTTCTTCATG ATTCTATTGA ACTCACAGTT
    GTGTTTGGAA CCCCAATAGA TGGTGATGTT TTTGGTGAAA ATCCTTCCAG AAAAATAGTT
    AGCCTGAGCT TTGAATCTCT CTTGGATGAG GAAAAAGCTC CACTCTCCTC ACGTTTAGTC
    CAAAGACTCA TATTCCAGTT TATTGAAAGT CAGGGATGCT GGCAGGAAAA GTGTCCCACA
    CTACAATACT TGCCTCAGGT GCTTCACGAT GTCTCTTTGG TGGTGAGTCG TTGTAGGATC
    TTGGGAGAGG AGATCGAATT TCTGGAGAGA TGGGGTGGAA AATACAATCT TCTCAAGACA
    GATATAATTG ACACAAATGT GAAGCTCCTC TTCTCCACTT CCACCGCTTT TGCAAAGTTT
    GAGTTGACAC TGTCTTTATC ATCCAACTAC CCATCTGCTT CACTGCCTTT CACTGTTGAA
    AAAAAAATTG GGAATATTGG ACATGAGGAA ATTTCTGCTG TTCTCTCTAA AGTACCTATT
    GGTTACCATT ATCTCCGAAG AATGGTGAGC TTGATTCATC AAAATCTGCT CCAGGACCCC
    AGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004941355.1
    CDS16..7341
    Translation

    Target ORF information:

    RefSeq Version XM_004941298.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus kinetochore scaffold 1 (KNL1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004941298.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGCTGTACA GCGCTGCCGC CAGGAAGGCG GTTTGGCGCG GCGCGCAGGC GCGGCGGTTA 
    GGTTTGAATT GTGAGGCGGC AGCGGCGGCC CAGTCCTATG GGTGCTGTGG GGCTGGGGGT
    CAGCGGCGGA GAGCGGCCGA GAGTGTACTG TTTTTTATAG GAGAAACACT TCTTGCAGAA
    ATGGACAAAA TTTACATGGA CTCTAACGTG GAGAACGATA ACACAGAGCA CATCAGAGGA
    AAACGACTGT CCTCTATATT AAAGGCTCCA CGAAATCCTC TTGATGATCT GGGAAATGGG
    AATGAACTCA CCCAGGATCT CAACATAGAA AAACGTAGGA AAAGCTCTCG ACGTGTCAGC
    TTTGCCAACA CCATAAACTG CAGGGTCTTC CATCGGGATC TTAAGAATAA TGCAGCAGAA
    AGAGAAAATG AAGAAGATAC GGAGAATCAT GTCCTGCTTA ATCAAAATGA AGAACCTGAA
    GCTGTTCCAT GTGAGATTAC TGGGATGAAC ACCTTGCTTC ATGCCCCTAT TCAGTCATTG
    GTGCAGCAGA CTGATGAGTG CCATGATGTG GACAATACTA TTCCGAGAAC AGATAGGCCT
    GATGCAACAG TCATCTTCTC AGATGAAAAT GAAATGGATA TGACAGCTAG TCACACTGCA
    GTGATTGCAC GTAACCTGAA TAACAGTGAA ACTGACAAAA CTGAGAAAAT AGATATTGCG
    TCATTTCTGG CTGAGTTAAA TTCTATCAAT GGAAAAGCAG AAAAAAACAA AGATTTTCAT
    TTTTCCTTTG ATGCTGCAGA CCGTTTTTGC CCATCTTTTG AACAGAAGGA AGATGCTACT
    ACAGGAAAGA AAATAAATTC CAATGAATTT TTCATGAACT CAAATGAAAA AGCACTTAAT
    CCTATTGAAG GACCTGAAAA AGAGAACCTT TTTTTTGTCC CTTCAGAAGT GTCAGAAAAC
    ATGGCACGAT CATCAGTGGA ATATGTATAT TCCCATGAAC AACTAGCCAC CTGCAATGTA
    ACAAAAATAT TTAGAGAGCA AGATGATGGA ATGGAAATAA CAAAGTGCCA AGCATCTGAT
    ATTCAAAATA TGCATTCTGG TCTTTCTGAG GTTCCGACAG AGCAGTTAGC ATCTGCAGAT
    GTTACTCAGG CATTTGCAGA TGATAGAATG GATATGACAA CTAATCACAC TGCAAAAATA
    CATTTTCCCT TTGCCATTGT AGGTAATCAA AGTTTAAATT CCAGAAAGGA TTTCTCATCC
    ATGGAGCTAG ATAACTACTG TATTGTAAAG AGGACATCTC ACCAGCAATT AATCCTAAAG
    CAAGACCCTC AACTTTGTAC ACGCAAAAAA ATCATAAATG TTGAAGACAC AGTAGATGCT
    ACAGCACTGC AGGCTTTTAA GCAGGAGAGC AGAACATTGT CTTCTATCCC AGGATCTGTT
    TCTTCTGAGA CTGTCTTCCG AGATGATAAA ACTGTTGTTT TTTCTCAATG TGATGACATG
    GAAATTACTG GGAATTACAG AGATGTACTC TATAATACAA GTACCAGAGG AATGAACAGT
    TCATGTAATA AAGCTTTTGA AAAAACAGTT AACAGAAGTT CATTGCTAGT AGAAAGACGA
    CATCCGGTAA ATAATGACAA TACAAGAAGT CTGTCTTTAG ATGATAGATC TTCTCCGTCA
    CGCAAGAATA GTGCACGTGA ACTATCTTCA GGTTCAGATG GACAAAATGA AAGAGTAACC
    CAAGATCACA GTACTGTTTC ATTGAACACT GGTTTCAATT CATGTACAAA TTCAATGTCT
    ACTGGTTCCT CTAAGGGTAG GCTGCAGCAC AGACTAGCAA ACTCCCTACT TGTTTCACTA
    CCAGGTGAGA AAACTGCAAT ATTTTCAGGA ACAGATGTGG ACTTGACTAA AAATTGTGTT
    GGCAAGGGTT ATAGAAAGAA TATTGAAAAT GAATTTCCTC CTGGATTGTC CAACTCTGTC
    ACTTGCAAAC CACATTTTCT TGATAGTAGG TCTGTATCTT CCAATTTAAG GGAGCAGGAA
    AAAATGGAAA TAACTAAATG TCAGACAGTT GTCAGTGGTG ATCAAAGCAT TGGGATAACC
    ACAGAAGCAG AACAAAGGCA TTGTAAAATA ATTCCAAGTA AGAACCAGAA CAATAATGTT
    AGTGATTCTT TGGATAGGGA CAAGGAAAAT CTTGAGGCAA TTGGTTTTGA TGTGAGTATT
    AGAAGAAGCC AGGCTATAGA AGCGAATGCT AAAGGTGTTG AGGTGGTAAC TGTCAATAAG
    AAGCCTGGAA AAACAGACTT TCAGGCTAGT AGTCTGAGTT CCTGTGCAAA GAGCAGGTAT
    TCACTCCAGG AGCAAAAGAG GGAAGTCTCT GAAAATATTG TCACTGACAA ATGTGCATTC
    ACATCTTTGC AAAGTGAAAA AGTATATGTA TCCCAGCCTT TGGGAAATAA ACTTCCAAAT
    TCTTCAGTTT CCTGCACAAA TGACAAAAGA GACATGTTTT CAGATGATGA AAACATGGAC
    ATAACCAAAG CTCATTCTTT GGTTGCTCCT TCTATGAATA TAGTACAAGA AAATGAGAAT
    ACTCACGTTC AAAATAATGT AAAAGCCAAA CAGCTGCAAA ACCAGACCTT CCAGTTTTCT
    GATAATTCTG GTATAGATCA TACAAGTCAG ACTGCAGCAA TAGAATGTGG AGATGTCAAA
    AGGAGCTCAA ATAAACAAAC TGTTGCTCAT TTAGCTCCAA GTGGTTTCTT TATTTCCAGT
    AATGAGCTTG CTCCCTCTGG AAAGAAAAGA GATGCACAAC CTGGAAATAC ACTGGGAATA
    ATCTCAGATT GCCAGAACTC TGATAGGCAT AAGAAAACAC ATCCTAGGAT AGTAGTAAAC
    AATGTATCAT CAACTGCAGA AGACCGTGAG AGTGATTTTT TGATCAGTAA AACAGTTTCT
    TTAGAAGGGG GTTTTAATAA CCCTCAGTCA GGTGATGACC TGTGCTTGAG GGGTGTTGAA
    GAGAGGTCAC TAGCCAGTAA TTCTAAACAA CTTAAGGGAA GTTCCCATCT GCCTTCCTTT
    TTAGAAAAGT CAGTTGTATT TTCCTCTGGT GAAAACATGG ACTTGACTGA AAACTCTGCA
    GTAGTGGCTC CTGATCAAAA CATCTGTACT ATTTTCCCAG GAAGAAAAGC AGCACCAGGA
    CGTATTGAAG ATAAAAGTAA AATATCCTTT AAAAAAGAGG TAATGATGAC AAACAGTCAG
    GAACAGCCTG GATGTGATAT GTACTCCTTG GTATCTGATA AGAAAATGTT AACTGTGACT
    GGTATAAAGC ATTTGCTTTC TGCAGATGAG AAAACTACCA TCTTTTCAGA AGGTGCTAAT
    ATGGACATCA CTGGAAATCA TACAGCTTCA GCAGACAACA AAACGATTTT GCAGCCTGAA
    ATTTTGAACA ATGACATATC CTTCATTTCT GAAGATAAAA CTTCTGTTTT TACAAAAAAT
    AACGACATGG AAATAAGTAG GCTTGATTCT GTTGCAACTG ATAATTCTAT GGAAAAGGCT
    GTATCTCAAG GGATGCTTAG CAGAACTAAT GGAGCTAGAA GGAAAAGCTT GAAGGGAACA
    ATAGGGGAAA AGACTGTTTT ATTTTCACTG AGTGAGAATA ATGACATGAG CATCACAGAG
    GGACTTACAG CAGCAATAAG TCATGAAATT GTCTCACAAA ATGAGGCAGG GGCTCCTTCT
    CCATCTTCAG CTCATCCTGT TACAAATGTT ACATTCACAT GTAACCAGGC TGACATTAAG
    TCTTGCTCTG CCAATAAAAT TACTGTAAAA GTTCCATGTG ATGATGCGTT GATCTCAGAT
    AAAGTTTTTG GACAGGAATT TCCTTCAAAC AGAGAAACTA CTGTGTTTGC TATGGATGAT
    ATGGAAATCA CTAAGGCCCA TAATGCATCT TTAAAAGAAA ATTTTCTGCA AAGTAAGCAA
    CCCAATCAAT CCGTTCCTTT AACTTCCTCA ATTATATTTA CAAGTGACCA GGCTGACATG
    GAAATGACTG AGTATCACCC TGTTGATGAA AGTGTTGAAA GGGATTTATG TGAGGATACA
    TTAAAGCTGC CCGCAGAGGT TCTACAGGAG GCTCTTTCAG GTAGCAAAAC AGTCATTTTT
    CCTTTGGATG AGAATATGGA CGTCACAAAA ACTCATGATG TATTAAGTGG TAAGATCAGT
    GTATGGAATG GAAGGTCCAT TCCAGCCATT TCTGCAGTTC CTGCTGATAA GACCATTGTA
    TTTCATCACA ACCAAGATGA TATGGAAATA ACTTCATCAC ATACTGTTTC AGTTAATAAC
    AACACTGAAG GATCTGAGAA TCAAAAAGCA TCACATAAAA GCACTCAACA ACCAGACTTG
    CGTAGCGTAT CATCATCCTC TTGCAGAGAT GAAATAGATT CTCTACAAAT AAAAGACCTG
    AATGGTGGGG ATCATAAAAA ATCTAATATT AAGTCCTCAA TTCCTTCTGC TTCATCGGCC
    TCAGCGTCAC CAACTGAGAA AGGACCTATC AAAGTCCACA GTGGCACAAT CCCAGATTCC
    ATTTATTCTA TCTCAATTCC AAAAGATGCT TTGGATGTCC AAGCACTACA GGATCTTGGC
    CTTTCCACAA AAAATTCAAT TCCTATAAAC AACGAGCAGG ATCTTTTACA TCCAGGAGCA
    ATGAAATCAA GGAGAGCATC TTTCAAGATG CCAGGTAATG ACCCCTTGGA CTGCAGTGAA
    GAAAGTGGAG TTGTAGTATC CCATGTTTTT CTTCCTGCTC AGCACTCAGA CTCTTTGAAG
    GATCCTCCAG ATGCACTTAG TACTCAGAGA AATCAAGTAT TGAAAGATGA TTCATCTAGA
    CCTGAAGATC TAGCCACAGA TTTAGCCATG GATGGAGCAG GCATTGTTCC AGTTTCTAAC
    AAGGAATCGG AGGAAAATGT GCTGTCAGCT GAAGGAGAGG CAACAGAATT CCAAATAAAC
    TCTGAACAAA CTAAGCAACT TTCAGTCAGC GATAGTCTGA GAGATCCAAT AGATCTTGCG
    CCTAAATTAT CAGGTATTAT AAATATTTGT TCAAAACTGG AAAATATTAG AAGGAAATCT
    GTAGTTGTTT CTGAAACTGT TCTTTCTGAC CACTTGCTGA AGTTACCAAC TCAGCCAGAG
    GATACCTTGA TGTTAGGAAG AAATAGCATA AATGAACAAA GGTTATGTTA TGCCAAAGAG
    CAGGAGAATG CATGTCTTGA ATCTGGAGCT GCTCCCATAG ATGCAAATTT AGGAATGGCA
    CTTAAGGACA AATATAAAGG AAGAAACTTC CCTCTAGGTA TCTTTCAGCC TAAACTACCA
    AACAGAAGAA ATCCTTCTGT TTCTAGTGTG CAAGACACAA CTGCAAAATC TTCAGGCAAA
    GTAGAATCAG CAGTTCCAGA AGTAAATCTG AACACCATTA AAGCTCCAGG TTACAACAAC
    GCTAGTAGGC AGAACTTCAG CCCTTCTCAG TTTATAGCAG AAGAATTTCT TCCTGTCTGC
    CCAGAAGAAA TGGATTCAAA TGATTCTGTA AGCTCTGAGT TTGTGGAAGA GGCTTGCAAT
    AACGGAAACA AAAGAGAAAT CCTCCACAAT GAAGAGATTC AAGTTGAAGA GACAAAAACT
    TGCAACAATG CAAAAAGAGC TTTGAAATGT GTTGAGGAGG ATCTTCAAAG TCTAAAGAGG
    CTCAAGATGG ATGAAATCGT GGTTGATGGG ACCTCCCAGG ACTTGCAGGT TACTTACGGC
    ACTGTACCTG AAAGCCAAGC AGAAGTTGCT GAAAGTGAAG ATTCTCCAAA TCTATCAGCT
    AAAAACCCTG ACTGTACACA TGCCAACAGC AGCAGCTCTC TAGATTCTGT TAAGGCTGAC
    ACAGAATTAA CAATTCAACG AAGCAGTCAA ATGGAATCGC AGCTTCTCAC AGATAGCATT
    TGTGAAGATA ATTTGTGGGA GAAATTTCAG AGTGGTGTCA TCACAGTTGC GGAGTTTTTT
    ACTCTTCTTC AAGTCCATAT TCCAATTCAG AAGCCCCGGC AGAGCCATAT TCCAGCCAAC
    TGTGCTGTCA GTGCTCCACC TACTCCAGAA GACCTGATTC TCAGCCAATA CGTTTATCGT
    CCCAAGATGC GTATTTATGA AGAAGACTGC CAGGTTCTTT CTCAGATGAT AGATGAATTA
    AAACAGTATG TAAGTGTCCA GGATCAACCA CTGGTGAATG TGAATAAGAG TTTGTGGGAA
    GTAATGAGAA CCTGCTCTGA CGAAGAGCTG AAGAGCTTTG GAGCAGAACT GAACAAAATG
    AAATCCCATT TCATCAAGGA AAGTAAAATC CTGGCTCACA ATGAGAAAGA GGCATTATAC
    AGCAAATTGC TGCAGAGTGC GCAGGAACAA TATGAAAAGC TCCAGTCAAG AACAGCAAAC
    ATGGATGAAT TGCTTAAGGA GGCAGAAAGT TGTCTTGCAG ATCTAAAAGC AGATTCTGAA
    TGGGAAGAAT GTGAAACAGA CTGCAGTGAT GATGAAATGA AGGGAGAGAA CTCAGAATCA
    AGAAAACTAG AAGAAGAATT GGGAAGCCTC AAAGCTCAAG AAGAGGAACT TCAAAGAGAA
    CTGTCAGATC TGGAGACTGA GAATGAACAA ATACTTGCTC AGATGAAGCA TCTACAGGAA
    GATGAAAAGA GCTGCCAGGA ACTCCTGGAG AGATGCAACT TCACTGAGTG GGAGATTAGT
    GAATGGAGTG AACTCCAGGC AGTCTTTAAC TTTCTTCATG ATTCTATTGA ACTCACAGTT
    GTGTTTGGAA CCCCAATAGA TGGTGATGTT TTTGGTGAAA ATCCTTCCAG AAAAATAGTT
    AGCCTGAGCT TTGAATCTCT CTTGGATGAG GAAAAAGCTC CACTCTCCTC ACGTTTAGTC
    CAAAGACTCA TATTCCAGTT TATTGAAAGT CAGGGATGCT GGCAGGAAAA GTGTCCCACA
    CTACAATACT TGCCTCAGGT GCTTCACGAT GTCTCTTTGG TGGTGAGTCG TTGTAGGATC
    TTGGGAGAGG AGATCGAATT TCTGGAGAGA TGGGGTGGAA AATACAATCT TCTCAAGACA
    GATATAATTG ACACAAATGT GAAGCTCCTC TTCTCCACTT CCACCGCTTT TGCAAAGTTT
    GAGTTGACAC TGTCTTTATC ATCCAACTAC CCATCTGCTT CACTGCCTTT CACTGTTGAA
    AAAAAAATTG GGAATATTGG ACATGAGGAA ATTTCTGCTG TTCTCTCTAA AGTACCTATT
    GGTTACCATT ATCTCCGAAG AATGGTGAGC TTGATTCATC AAAATCTGCT CCAGGACCCC
    AGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.