NOS1 cDNA ORF clone, Homo sapiens(human)

The following NOS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NOS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu21527 NM_001204214.1
Latest version!
Homo sapiens nitric oxide synthase 1 (NOS1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu21527 NM_001204213.1
Latest version!
Homo sapiens nitric oxide synthase 1 (NOS1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu21020 NM_000620.5
Latest version!
Homo sapiens nitric oxide synthase 1 (NOS1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OHu21540 NM_001204218.1
Latest version!
Homo sapiens nitric oxide synthase 1 (NOS1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OHu21020 XM_017019347.1
Latest version!
Homo sapiens nitric oxide synthase 1 (NOS1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OHu21540 XM_011538398.2
Latest version!
Homo sapiens nitric oxide synthase 1 (NOS1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OHu21540 XM_017019346.1
Latest version!
Homo sapiens nitric oxide synthase 1 (NOS1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OHu21540 XM_017019345.1
Latest version!
Homo sapiens nitric oxide synthase 1 (NOS1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
View Old Accession Versions >>
OHu21020 NM_000620.4 Homo sapiens nitric oxide synthase 1 (NOS1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu21527
    Clone ID Related Accession (Same CDS sequence) NM_001204213.1 , NM_001204214.1
    Accession Version NM_001204213.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3297bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-27
    Organism Homo sapiens(human)
    Product nitric oxide synthase, brain isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC068799.14, AK294435.1, U17327.1, AK307481.1, BC033208.1, AC026364.36 and BE207961.1. On Aug 31, 2013 this sequence version replaced XM_005253886.1. Transcript Variant: This variant (3, also known as TnNOS) contains 2 unique alternate exons (Tex 1 and Tex 2) at the 5' end compared to variant 1, resulting in translation initiation from a downstream AUG, and an isoform (isoform 3, also known as nNOSgamma) with a shorter N-terminus compared to isoform 1. This variant is specifically expressed in the testis, and the encoded isoform has catalytic activity (PMID:9111048). Variants 3 and 4 encode the same isoform. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03465413 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGGCTCCA TCATGCATCC TTCTCAGCAT GCAAGGAGGC CTGAAGACGT CCGCACAAAA 
    GGACAGCTCT TCCCTCTCGC CAAAGAGTTT ATTGATCAAT ACTATTCATC AATTAAAAGA
    TTTGGCTCCA AAGCCCACAT GGAAAGGCTG GAAGAGGTGA ACAAAGAGAT CGACACCACT
    AGCACTTACC AGCTCAAGGA CACAGAGCTC ATCTATGGGG CCAAGCACGC CTGGCGGAAT
    GCCTCGCGCT GTGTGGGCAG GATCCAGTGG TCCAAGCTGC AGGTATTCGA TGCCCGTGAC
    TGCACCACGG CCCACGGGAT GTTCAACTAC ATCTGTAACC ATGTCAAGTA TGCCACCAAC
    AAAGGGAACC TCAGGTCTGC CATCACCATA TTCCCCCAGA GGACAGACGG CAAGCACGAC
    TTCCGAGTCT GGAACTCCCA GCTCATCCGC TACGCTGGCT ACAAGCAGCC TGACGGCTCC
    ACCCTGGGGG ACCCAGCCAA TGTGCAGTTC ACAGAGATAT GCATACAGCA GGGCTGGAAA
    CCGCCTAGAG GCCGCTTCGA TGTCCTGCCG CTCCTGCTTC AGGCCAACGG CAATGACCCT
    GAGCTCTTCC AGATTCCTCC AGAGCTGGTG TTGGAAGTTC CCATCAGGCA CCCCAAGTTT
    GAGTGGTTCA AGGACCTGGG GCTGAAGTGG TACGGCCTCC CCGCCGTGTC CAACATGCTC
    CTAGAGATTG GCGGCCTGGA GTTCAGCGCC TGTCCCTTCA GTGGCTGGTA CATGGGCACA
    GAGATTGGTG TCCGCGACTA CTGTGACAAC TCCCGCTACA ATATCCTGGA GGAAGTGGCC
    AAGAAGATGA ACTTAGACAT GAGGAAGACG TCCTCCCTGT GGAAGGACCA GGCGCTGGTG
    GAGATCAATA TCGCGGTTCT CTATAGCTTC CAGAGTGACA AAGTGACCAT TGTTGACCAT
    CACTCCGCCA CCGAGTCCTT CATTAAGCAC ATGGAGAATG AGTACCGCTG CCGGGGGGGC
    TGCCCTGCCG ACTGGGTGTG GATCGTGCCC CCCATGTCCG GAAGCATCAC CCCTGTGTTC
    CACCAGGAGA TGCTCAACTA CCGGCTCACC CCCTCCTTCG AATACCAGCC TGATCCCTGG
    AACACGCATG TCTGGAAAGG CACCAACGGG ACCCCCACAA AGCGGCGAGC CATTGGCTTC
    AAGAAGCTAG CAGAAGCTGT CAAGTTCTCG GCCAAGCTGA TGGGGCAGGC TATGGCCAAG
    AGGGTGAAAG CGACCATCCT CTATGCCACA GAGACAGGCA AATCGCAAGC TTATGCCAAG
    ACCTTGTGTG AGATCTTCAA ACACGCCTTT GATGCCAAGG TGATGTCCAT GGAAGAATAT
    GACATTGTGC ACCTGGAACA TGAAACTCTG GTCCTTGTGG TCACCAGCAC CTTTGGCAAT
    GGAGATCCCC CTGAGAATGG GGAGAAATTC GGCTGTGCTT TGATGGAAAT GAGGCACCCC
    AACTCTGTGC AGGAAGAAAG GAAGAGCTAC AAGGTCCGAT TCAACAGCGT CTCCTCCTAC
    TCTGACTCCC AAAAATCATC AGGCGATGGG CCCGACCTCA GAGACAACTT TGAGAGTGCT
    GGACCCCTGG CCAATGTGAG GTTCTCAGTT TTTGGCCTCG GCTCACGAGC ATACCCTCAC
    TTTTGCGCCT TCGGACACGC TGTGGACACC CTCCTGGAAG AACTGGGAGG GGAGAGGATC
    CTGAAGATGA GGGAAGGGGA TGAGCTCTGT GGGCAGGAAG AGGCTTTCAG GACCTGGGCC
    AAGAAGGTCT TCAAGGCAGC CTGTGATGTC TTCTGTGTGG GAGATGATGT CAACATTGAA
    AAGGCCAACA ATTCCCTCAT CAGCAATGAT CGCAGCTGGA AGAGAAACAA GTTCCGCCTC
    ACCTTTGTGG CCGAAGCTCC AGAACTCACA CAAGGTCTAT CCAATGTCCA CAAAAAGCGA
    GTCTCAGCTG CCCGGCTCCT TAGCCGTCAA AACCTCCAGA GCCCTAAATC CAGTCGGTCA
    ACTATCTTCG TGCGTCTCCA CACCAACGGG AGCCAGGAGC TGCAGTACCA GCCTGGGGAC
    CACCTGGGTG TCTTCCCTGG CAACCACGAG GACCTCGTGA ATGCCCTGAT CGAGCGGCTG
    GAGGACGCGC CGCCTGTCAA CCAGATGGTG AAAGTGGAAC TGCTGGAGGA GCGGAACACG
    GCTTTAGGTG TCATCAGTAA CTGGACAGAC GAGCTCCGCC TCCCGCCCTG CACCATCTTC
    CAGGCCTTCA AGTACTACCT GGACATCACC ACGCCACCAA CGCCTCTGCA GCTGCAGCAG
    TTTGCCTCCC TAGCTACCAG CGAGAAGGAG AAGCAGCGTC TGCTGGTCCT CAGCAAGGGT
    TTGCAGGAGT ACGAGGAATG GAAATGGGGC AAGAACCCCA CCATCGTGGA GGTGCTGGAG
    GAGTTCCCAT CTATCCAGAT GCCGGCCACC CTGCTCCTGA CCCAGCTGTC CCTGCTGCAG
    CCCCGCTACT ATTCCATCAG CTCCTCCCCA GACATGTACC CTGATGAAGT GCACCTCACT
    GTGGCCATCG TTTCCTACCG CACTCGAGAT GGAGAAGGAC CAATTCACCA CGGCGTATGC
    TCCTCCTGGC TCAACCGGAT ACAGGCTGAC GAACTGGTCC CCTGTTTCGT GAGAGGAGCA
    CCCAGCTTCC ACCTGCCCCG GAACCCCCAA GTCCCCTGCA TCCTCGTTGG ACCAGGCACC
    GGCATTGCCC CTTTCCGAAG CTTCTGGCAA CAGCGGCAAT TTGATATCCA ACACAAAGGA
    ATGAACCCCT GCCCCATGGT CCTGGTCTTC GGGTGCCGGC AATCCAAGAT AGATCATATC
    TACAGGGAAG AGACCCTGCA GGCCAAGAAC AAGGGGGTCT TCAGAGAGCT GTACACGGCT
    TACTCCCGGG AGCCAGACAA ACCAAAGAAG TACGTGCAGG ACATCCTGCA GGAGCAGCTG
    GCGGAGTCTG TGTACCGAGC CCTGAAGGAG CAAGGGGGCC ACATATACGT CTGTGGGGAC
    GTCACCATGG CTGCTGATGT CCTCAAAGCC ATCCAGCGCA TCATGACCCA GCAGGGGAAG
    CTCTCGGCAG AGGACGCCGG CGTATTCATC AGCCGGATGA GGGATGACAA CCGATACCAT
    GAGGATATTT TTGGAGTCAC CCTGCGAACG TACGAAGTGA CCAACCGCCT TAGATCTGAG
    TCCATTGCCT TCATTGAAGA GAGCAAAAAA GACACCGATG AGGTTTTCAG CTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001191142.1
    CDS312..3608
    Translation

    Target ORF information:

    RefSeq Version NM_001204213.1
    Organism Homo sapiens(human)
    Definition Homo sapiens nitric oxide synthase 1 (NOS1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001204213.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGGCTCCA TCATGCATCC TTCTCAGCAT GCAAGGAGGC CTGAAGACGT CCGCACAAAA 
    GGACAGCTCT TCCCTCTCGC CAAAGAGTTT ATTGATCAAT ACTATTCATC AATTAAAAGA
    TTTGGCTCCA AAGCCCACAT GGAAAGGCTG GAAGAGGTGA ACAAAGAGAT CGACACCACT
    AGCACTTACC AGCTCAAGGA CACAGAGCTC ATCTATGGGG CCAAGCACGC CTGGCGGAAT
    GCCTCGCGCT GTGTGGGCAG GATCCAGTGG TCCAAGCTGC AGGTATTCGA TGCCCGTGAC
    TGCACCACGG CCCACGGGAT GTTCAACTAC ATCTGTAACC ATGTCAAGTA TGCCACCAAC
    AAAGGGAACC TCAGGTCTGC CATCACCATA TTCCCCCAGA GGACAGACGG CAAGCACGAC
    TTCCGAGTCT GGAACTCCCA GCTCATCCGC TACGCTGGCT ACAAGCAGCC TGACGGCTCC
    ACCCTGGGGG ACCCAGCCAA TGTGCAGTTC ACAGAGATAT GCATACAGCA GGGCTGGAAA
    CCGCCTAGAG GCCGCTTCGA TGTCCTGCCG CTCCTGCTTC AGGCCAACGG CAATGACCCT
    GAGCTCTTCC AGATTCCTCC AGAGCTGGTG TTGGAAGTTC CCATCAGGCA CCCCAAGTTT
    GAGTGGTTCA AGGACCTGGG GCTGAAGTGG TACGGCCTCC CCGCCGTGTC CAACATGCTC
    CTAGAGATTG GCGGCCTGGA GTTCAGCGCC TGTCCCTTCA GTGGCTGGTA CATGGGCACA
    GAGATTGGTG TCCGCGACTA CTGTGACAAC TCCCGCTACA ATATCCTGGA GGAAGTGGCC
    AAGAAGATGA ACTTAGACAT GAGGAAGACG TCCTCCCTGT GGAAGGACCA GGCGCTGGTG
    GAGATCAATA TCGCGGTTCT CTATAGCTTC CAGAGTGACA AAGTGACCAT TGTTGACCAT
    CACTCCGCCA CCGAGTCCTT CATTAAGCAC ATGGAGAATG AGTACCGCTG CCGGGGGGGC
    TGCCCTGCCG ACTGGGTGTG GATCGTGCCC CCCATGTCCG GAAGCATCAC CCCTGTGTTC
    CACCAGGAGA TGCTCAACTA CCGGCTCACC CCCTCCTTCG AATACCAGCC TGATCCCTGG
    AACACGCATG TCTGGAAAGG CACCAACGGG ACCCCCACAA AGCGGCGAGC CATTGGCTTC
    AAGAAGCTAG CAGAAGCTGT CAAGTTCTCG GCCAAGCTGA TGGGGCAGGC TATGGCCAAG
    AGGGTGAAAG CGACCATCCT CTATGCCACA GAGACAGGCA AATCGCAAGC TTATGCCAAG
    ACCTTGTGTG AGATCTTCAA ACACGCCTTT GATGCCAAGG TGATGTCCAT GGAAGAATAT
    GACATTGTGC ACCTGGAACA TGAAACTCTG GTCCTTGTGG TCACCAGCAC CTTTGGCAAT
    GGAGATCCCC CTGAGAATGG GGAGAAATTC GGCTGTGCTT TGATGGAAAT GAGGCACCCC
    AACTCTGTGC AGGAAGAAAG GAAGAGCTAC AAGGTCCGAT TCAACAGCGT CTCCTCCTAC
    TCTGACTCCC AAAAATCATC AGGCGATGGG CCCGACCTCA GAGACAACTT TGAGAGTGCT
    GGACCCCTGG CCAATGTGAG GTTCTCAGTT TTTGGCCTCG GCTCACGAGC ATACCCTCAC
    TTTTGCGCCT TCGGACACGC TGTGGACACC CTCCTGGAAG AACTGGGAGG GGAGAGGATC
    CTGAAGATGA GGGAAGGGGA TGAGCTCTGT GGGCAGGAAG AGGCTTTCAG GACCTGGGCC
    AAGAAGGTCT TCAAGGCAGC CTGTGATGTC TTCTGTGTGG GAGATGATGT CAACATTGAA
    AAGGCCAACA ATTCCCTCAT CAGCAATGAT CGCAGCTGGA AGAGAAACAA GTTCCGCCTC
    ACCTTTGTGG CCGAAGCTCC AGAACTCACA CAAGGTCTAT CCAATGTCCA CAAAAAGCGA
    GTCTCAGCTG CCCGGCTCCT TAGCCGTCAA AACCTCCAGA GCCCTAAATC CAGTCGGTCA
    ACTATCTTCG TGCGTCTCCA CACCAACGGG AGCCAGGAGC TGCAGTACCA GCCTGGGGAC
    CACCTGGGTG TCTTCCCTGG CAACCACGAG GACCTCGTGA ATGCCCTGAT CGAGCGGCTG
    GAGGACGCGC CGCCTGTCAA CCAGATGGTG AAAGTGGAAC TGCTGGAGGA GCGGAACACG
    GCTTTAGGTG TCATCAGTAA CTGGACAGAC GAGCTCCGCC TCCCGCCCTG CACCATCTTC
    CAGGCCTTCA AGTACTACCT GGACATCACC ACGCCACCAA CGCCTCTGCA GCTGCAGCAG
    TTTGCCTCCC TAGCTACCAG CGAGAAGGAG AAGCAGCGTC TGCTGGTCCT CAGCAAGGGT
    TTGCAGGAGT ACGAGGAATG GAAATGGGGC AAGAACCCCA CCATCGTGGA GGTGCTGGAG
    GAGTTCCCAT CTATCCAGAT GCCGGCCACC CTGCTCCTGA CCCAGCTGTC CCTGCTGCAG
    CCCCGCTACT ATTCCATCAG CTCCTCCCCA GACATGTACC CTGATGAAGT GCACCTCACT
    GTGGCCATCG TTTCCTACCG CACTCGAGAT GGAGAAGGAC CAATTCACCA CGGCGTATGC
    TCCTCCTGGC TCAACCGGAT ACAGGCTGAC GAACTGGTCC CCTGTTTCGT GAGAGGAGCA
    CCCAGCTTCC ACCTGCCCCG GAACCCCCAA GTCCCCTGCA TCCTCGTTGG ACCAGGCACC
    GGCATTGCCC CTTTCCGAAG CTTCTGGCAA CAGCGGCAAT TTGATATCCA ACACAAAGGA
    ATGAACCCCT GCCCCATGGT CCTGGTCTTC GGGTGCCGGC AATCCAAGAT AGATCATATC
    TACAGGGAAG AGACCCTGCA GGCCAAGAAC AAGGGGGTCT TCAGAGAGCT GTACACGGCT
    TACTCCCGGG AGCCAGACAA ACCAAAGAAG TACGTGCAGG ACATCCTGCA GGAGCAGCTG
    GCGGAGTCTG TGTACCGAGC CCTGAAGGAG CAAGGGGGCC ACATATACGT CTGTGGGGAC
    GTCACCATGG CTGCTGATGT CCTCAAAGCC ATCCAGCGCA TCATGACCCA GCAGGGGAAG
    CTCTCGGCAG AGGACGCCGG CGTATTCATC AGCCGGATGA GGGATGACAA CCGATACCAT
    GAGGATATTT TTGGAGTCAC CCTGCGAACG TACGAAGTGA CCAACCGCCT TAGATCTGAG
    TCCATTGCCT TCATTGAAGA GAGCAAAAAA GACACCGATG AGGTTTTCAG CTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu21527
    Clone ID Related Accession (Same CDS sequence) NM_001204213.1 , NM_001204214.1
    Accession Version NM_001204214.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3297bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-27
    Organism Homo sapiens(human)
    Product nitric oxide synthase, brain isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC068799.14, AK294435.1, U17327.1, AK307481.1, BC033208.1, AC026364.36 and BE207961.1. Transcript Variant: This variant (4, also known as TnNOSb) contains 2 unique alternate exons (Tex 1b and Tex 2) at the 5' end compared to variant 1, resulting in translation initiation from a downstream AUG, and an isoform (3, also known as nNOSgamma) with a shorter N-terminus compared to isoform 1. This variant is specifically expressed in the testis, and the encoded isoform has catalytic activity (PMID:9111048). Variants 3 and 4 encode the same isoform. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03465413 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGGCTCCA TCATGCATCC TTCTCAGCAT GCAAGGAGGC CTGAAGACGT CCGCACAAAA 
    GGACAGCTCT TCCCTCTCGC CAAAGAGTTT ATTGATCAAT ACTATTCATC AATTAAAAGA
    TTTGGCTCCA AAGCCCACAT GGAAAGGCTG GAAGAGGTGA ACAAAGAGAT CGACACCACT
    AGCACTTACC AGCTCAAGGA CACAGAGCTC ATCTATGGGG CCAAGCACGC CTGGCGGAAT
    GCCTCGCGCT GTGTGGGCAG GATCCAGTGG TCCAAGCTGC AGGTATTCGA TGCCCGTGAC
    TGCACCACGG CCCACGGGAT GTTCAACTAC ATCTGTAACC ATGTCAAGTA TGCCACCAAC
    AAAGGGAACC TCAGGTCTGC CATCACCATA TTCCCCCAGA GGACAGACGG CAAGCACGAC
    TTCCGAGTCT GGAACTCCCA GCTCATCCGC TACGCTGGCT ACAAGCAGCC TGACGGCTCC
    ACCCTGGGGG ACCCAGCCAA TGTGCAGTTC ACAGAGATAT GCATACAGCA GGGCTGGAAA
    CCGCCTAGAG GCCGCTTCGA TGTCCTGCCG CTCCTGCTTC AGGCCAACGG CAATGACCCT
    GAGCTCTTCC AGATTCCTCC AGAGCTGGTG TTGGAAGTTC CCATCAGGCA CCCCAAGTTT
    GAGTGGTTCA AGGACCTGGG GCTGAAGTGG TACGGCCTCC CCGCCGTGTC CAACATGCTC
    CTAGAGATTG GCGGCCTGGA GTTCAGCGCC TGTCCCTTCA GTGGCTGGTA CATGGGCACA
    GAGATTGGTG TCCGCGACTA CTGTGACAAC TCCCGCTACA ATATCCTGGA GGAAGTGGCC
    AAGAAGATGA ACTTAGACAT GAGGAAGACG TCCTCCCTGT GGAAGGACCA GGCGCTGGTG
    GAGATCAATA TCGCGGTTCT CTATAGCTTC CAGAGTGACA AAGTGACCAT TGTTGACCAT
    CACTCCGCCA CCGAGTCCTT CATTAAGCAC ATGGAGAATG AGTACCGCTG CCGGGGGGGC
    TGCCCTGCCG ACTGGGTGTG GATCGTGCCC CCCATGTCCG GAAGCATCAC CCCTGTGTTC
    CACCAGGAGA TGCTCAACTA CCGGCTCACC CCCTCCTTCG AATACCAGCC TGATCCCTGG
    AACACGCATG TCTGGAAAGG CACCAACGGG ACCCCCACAA AGCGGCGAGC CATTGGCTTC
    AAGAAGCTAG CAGAAGCTGT CAAGTTCTCG GCCAAGCTGA TGGGGCAGGC TATGGCCAAG
    AGGGTGAAAG CGACCATCCT CTATGCCACA GAGACAGGCA AATCGCAAGC TTATGCCAAG
    ACCTTGTGTG AGATCTTCAA ACACGCCTTT GATGCCAAGG TGATGTCCAT GGAAGAATAT
    GACATTGTGC ACCTGGAACA TGAAACTCTG GTCCTTGTGG TCACCAGCAC CTTTGGCAAT
    GGAGATCCCC CTGAGAATGG GGAGAAATTC GGCTGTGCTT TGATGGAAAT GAGGCACCCC
    AACTCTGTGC AGGAAGAAAG GAAGAGCTAC AAGGTCCGAT TCAACAGCGT CTCCTCCTAC
    TCTGACTCCC AAAAATCATC AGGCGATGGG CCCGACCTCA GAGACAACTT TGAGAGTGCT
    GGACCCCTGG CCAATGTGAG GTTCTCAGTT TTTGGCCTCG GCTCACGAGC ATACCCTCAC
    TTTTGCGCCT TCGGACACGC TGTGGACACC CTCCTGGAAG AACTGGGAGG GGAGAGGATC
    CTGAAGATGA GGGAAGGGGA TGAGCTCTGT GGGCAGGAAG AGGCTTTCAG GACCTGGGCC
    AAGAAGGTCT TCAAGGCAGC CTGTGATGTC TTCTGTGTGG GAGATGATGT CAACATTGAA
    AAGGCCAACA ATTCCCTCAT CAGCAATGAT CGCAGCTGGA AGAGAAACAA GTTCCGCCTC
    ACCTTTGTGG CCGAAGCTCC AGAACTCACA CAAGGTCTAT CCAATGTCCA CAAAAAGCGA
    GTCTCAGCTG CCCGGCTCCT TAGCCGTCAA AACCTCCAGA GCCCTAAATC CAGTCGGTCA
    ACTATCTTCG TGCGTCTCCA CACCAACGGG AGCCAGGAGC TGCAGTACCA GCCTGGGGAC
    CACCTGGGTG TCTTCCCTGG CAACCACGAG GACCTCGTGA ATGCCCTGAT CGAGCGGCTG
    GAGGACGCGC CGCCTGTCAA CCAGATGGTG AAAGTGGAAC TGCTGGAGGA GCGGAACACG
    GCTTTAGGTG TCATCAGTAA CTGGACAGAC GAGCTCCGCC TCCCGCCCTG CACCATCTTC
    CAGGCCTTCA AGTACTACCT GGACATCACC ACGCCACCAA CGCCTCTGCA GCTGCAGCAG
    TTTGCCTCCC TAGCTACCAG CGAGAAGGAG AAGCAGCGTC TGCTGGTCCT CAGCAAGGGT
    TTGCAGGAGT ACGAGGAATG GAAATGGGGC AAGAACCCCA CCATCGTGGA GGTGCTGGAG
    GAGTTCCCAT CTATCCAGAT GCCGGCCACC CTGCTCCTGA CCCAGCTGTC CCTGCTGCAG
    CCCCGCTACT ATTCCATCAG CTCCTCCCCA GACATGTACC CTGATGAAGT GCACCTCACT
    GTGGCCATCG TTTCCTACCG CACTCGAGAT GGAGAAGGAC CAATTCACCA CGGCGTATGC
    TCCTCCTGGC TCAACCGGAT ACAGGCTGAC GAACTGGTCC CCTGTTTCGT GAGAGGAGCA
    CCCAGCTTCC ACCTGCCCCG GAACCCCCAA GTCCCCTGCA TCCTCGTTGG ACCAGGCACC
    GGCATTGCCC CTTTCCGAAG CTTCTGGCAA CAGCGGCAAT TTGATATCCA ACACAAAGGA
    ATGAACCCCT GCCCCATGGT CCTGGTCTTC GGGTGCCGGC AATCCAAGAT AGATCATATC
    TACAGGGAAG AGACCCTGCA GGCCAAGAAC AAGGGGGTCT TCAGAGAGCT GTACACGGCT
    TACTCCCGGG AGCCAGACAA ACCAAAGAAG TACGTGCAGG ACATCCTGCA GGAGCAGCTG
    GCGGAGTCTG TGTACCGAGC CCTGAAGGAG CAAGGGGGCC ACATATACGT CTGTGGGGAC
    GTCACCATGG CTGCTGATGT CCTCAAAGCC ATCCAGCGCA TCATGACCCA GCAGGGGAAG
    CTCTCGGCAG AGGACGCCGG CGTATTCATC AGCCGGATGA GGGATGACAA CCGATACCAT
    GAGGATATTT TTGGAGTCAC CCTGCGAACG TACGAAGTGA CCAACCGCCT TAGATCTGAG
    TCCATTGCCT TCATTGAAGA GAGCAAAAAA GACACCGATG AGGTTTTCAG CTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001191143.1
    CDS307..3603
    Translation

    Target ORF information:

    RefSeq Version NM_001204214.1
    Organism Homo sapiens(human)
    Definition Homo sapiens nitric oxide synthase 1 (NOS1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001204214.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGGCTCCA TCATGCATCC TTCTCAGCAT GCAAGGAGGC CTGAAGACGT CCGCACAAAA 
    GGACAGCTCT TCCCTCTCGC CAAAGAGTTT ATTGATCAAT ACTATTCATC AATTAAAAGA
    TTTGGCTCCA AAGCCCACAT GGAAAGGCTG GAAGAGGTGA ACAAAGAGAT CGACACCACT
    AGCACTTACC AGCTCAAGGA CACAGAGCTC ATCTATGGGG CCAAGCACGC CTGGCGGAAT
    GCCTCGCGCT GTGTGGGCAG GATCCAGTGG TCCAAGCTGC AGGTATTCGA TGCCCGTGAC
    TGCACCACGG CCCACGGGAT GTTCAACTAC ATCTGTAACC ATGTCAAGTA TGCCACCAAC
    AAAGGGAACC TCAGGTCTGC CATCACCATA TTCCCCCAGA GGACAGACGG CAAGCACGAC
    TTCCGAGTCT GGAACTCCCA GCTCATCCGC TACGCTGGCT ACAAGCAGCC TGACGGCTCC
    ACCCTGGGGG ACCCAGCCAA TGTGCAGTTC ACAGAGATAT GCATACAGCA GGGCTGGAAA
    CCGCCTAGAG GCCGCTTCGA TGTCCTGCCG CTCCTGCTTC AGGCCAACGG CAATGACCCT
    GAGCTCTTCC AGATTCCTCC AGAGCTGGTG TTGGAAGTTC CCATCAGGCA CCCCAAGTTT
    GAGTGGTTCA AGGACCTGGG GCTGAAGTGG TACGGCCTCC CCGCCGTGTC CAACATGCTC
    CTAGAGATTG GCGGCCTGGA GTTCAGCGCC TGTCCCTTCA GTGGCTGGTA CATGGGCACA
    GAGATTGGTG TCCGCGACTA CTGTGACAAC TCCCGCTACA ATATCCTGGA GGAAGTGGCC
    AAGAAGATGA ACTTAGACAT GAGGAAGACG TCCTCCCTGT GGAAGGACCA GGCGCTGGTG
    GAGATCAATA TCGCGGTTCT CTATAGCTTC CAGAGTGACA AAGTGACCAT TGTTGACCAT
    CACTCCGCCA CCGAGTCCTT CATTAAGCAC ATGGAGAATG AGTACCGCTG CCGGGGGGGC
    TGCCCTGCCG ACTGGGTGTG GATCGTGCCC CCCATGTCCG GAAGCATCAC CCCTGTGTTC
    CACCAGGAGA TGCTCAACTA CCGGCTCACC CCCTCCTTCG AATACCAGCC TGATCCCTGG
    AACACGCATG TCTGGAAAGG CACCAACGGG ACCCCCACAA AGCGGCGAGC CATTGGCTTC
    AAGAAGCTAG CAGAAGCTGT CAAGTTCTCG GCCAAGCTGA TGGGGCAGGC TATGGCCAAG
    AGGGTGAAAG CGACCATCCT CTATGCCACA GAGACAGGCA AATCGCAAGC TTATGCCAAG
    ACCTTGTGTG AGATCTTCAA ACACGCCTTT GATGCCAAGG TGATGTCCAT GGAAGAATAT
    GACATTGTGC ACCTGGAACA TGAAACTCTG GTCCTTGTGG TCACCAGCAC CTTTGGCAAT
    GGAGATCCCC CTGAGAATGG GGAGAAATTC GGCTGTGCTT TGATGGAAAT GAGGCACCCC
    AACTCTGTGC AGGAAGAAAG GAAGAGCTAC AAGGTCCGAT TCAACAGCGT CTCCTCCTAC
    TCTGACTCCC AAAAATCATC AGGCGATGGG CCCGACCTCA GAGACAACTT TGAGAGTGCT
    GGACCCCTGG CCAATGTGAG GTTCTCAGTT TTTGGCCTCG GCTCACGAGC ATACCCTCAC
    TTTTGCGCCT TCGGACACGC TGTGGACACC CTCCTGGAAG AACTGGGAGG GGAGAGGATC
    CTGAAGATGA GGGAAGGGGA TGAGCTCTGT GGGCAGGAAG AGGCTTTCAG GACCTGGGCC
    AAGAAGGTCT TCAAGGCAGC CTGTGATGTC TTCTGTGTGG GAGATGATGT CAACATTGAA
    AAGGCCAACA ATTCCCTCAT CAGCAATGAT CGCAGCTGGA AGAGAAACAA GTTCCGCCTC
    ACCTTTGTGG CCGAAGCTCC AGAACTCACA CAAGGTCTAT CCAATGTCCA CAAAAAGCGA
    GTCTCAGCTG CCCGGCTCCT TAGCCGTCAA AACCTCCAGA GCCCTAAATC CAGTCGGTCA
    ACTATCTTCG TGCGTCTCCA CACCAACGGG AGCCAGGAGC TGCAGTACCA GCCTGGGGAC
    CACCTGGGTG TCTTCCCTGG CAACCACGAG GACCTCGTGA ATGCCCTGAT CGAGCGGCTG
    GAGGACGCGC CGCCTGTCAA CCAGATGGTG AAAGTGGAAC TGCTGGAGGA GCGGAACACG
    GCTTTAGGTG TCATCAGTAA CTGGACAGAC GAGCTCCGCC TCCCGCCCTG CACCATCTTC
    CAGGCCTTCA AGTACTACCT GGACATCACC ACGCCACCAA CGCCTCTGCA GCTGCAGCAG
    TTTGCCTCCC TAGCTACCAG CGAGAAGGAG AAGCAGCGTC TGCTGGTCCT CAGCAAGGGT
    TTGCAGGAGT ACGAGGAATG GAAATGGGGC AAGAACCCCA CCATCGTGGA GGTGCTGGAG
    GAGTTCCCAT CTATCCAGAT GCCGGCCACC CTGCTCCTGA CCCAGCTGTC CCTGCTGCAG
    CCCCGCTACT ATTCCATCAG CTCCTCCCCA GACATGTACC CTGATGAAGT GCACCTCACT
    GTGGCCATCG TTTCCTACCG CACTCGAGAT GGAGAAGGAC CAATTCACCA CGGCGTATGC
    TCCTCCTGGC TCAACCGGAT ACAGGCTGAC GAACTGGTCC CCTGTTTCGT GAGAGGAGCA
    CCCAGCTTCC ACCTGCCCCG GAACCCCCAA GTCCCCTGCA TCCTCGTTGG ACCAGGCACC
    GGCATTGCCC CTTTCCGAAG CTTCTGGCAA CAGCGGCAAT TTGATATCCA ACACAAAGGA
    ATGAACCCCT GCCCCATGGT CCTGGTCTTC GGGTGCCGGC AATCCAAGAT AGATCATATC
    TACAGGGAAG AGACCCTGCA GGCCAAGAAC AAGGGGGTCT TCAGAGAGCT GTACACGGCT
    TACTCCCGGG AGCCAGACAA ACCAAAGAAG TACGTGCAGG ACATCCTGCA GGAGCAGCTG
    GCGGAGTCTG TGTACCGAGC CCTGAAGGAG CAAGGGGGCC ACATATACGT CTGTGGGGAC
    GTCACCATGG CTGCTGATGT CCTCAAAGCC ATCCAGCGCA TCATGACCCA GCAGGGGAAG
    CTCTCGGCAG AGGACGCCGG CGTATTCATC AGCCGGATGA GGGATGACAA CCGATACCAT
    GAGGATATTT TTGGAGTCAC CCTGCGAACG TACGAAGTGA CCAACCGCCT TAGATCTGAG
    TCCATTGCCT TCATTGAAGA GAGCAAAAAA GACACCGATG AGGTTTTCAG CTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu21020
    Clone ID Related Accession (Same CDS sequence) NM_000620.4 , NM_000620.5 , XM_017019347.1
    Accession Version NM_000620.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4305bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-28
    Organism Homo sapiens(human)
    Product nitric oxide synthase, brain isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK307481.1, U17327.1, AK294435.1, BC033208.1, AC026364.36 and BE207961.1. This sequence is a reference standard in the RefSeqGene project. On Feb 18, 2011 this sequence version replaced NM_000620.3. Transcript Variant: This variant (1) represents the predominant transcript, and encodes isoform 1 (also known as nNOS). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU753241.1, U17327.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03465402, SAMN03465403 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAGGATC ACATGTTCGG TGTTCAGCAA ATCCAGCCCA ATGTCATTTC TGTTCGTCTC 
    TTCAAGCGCA AAGTTGGGGG CCTGGGATTT CTGGTGAAGG AGCGGGTCAG TAAGCCGCCC
    GTGATCATCT CTGACCTGAT TCGTGGGGGC GCCGCAGAGC AGAGTGGCCT CATCCAGGCC
    GGAGACATCA TTCTTGCGGT CAACGGCCGG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC
    CTGGAGGTAC TCAGAGGCAT TGCCTCTGAG ACCCACGTGG TCCTCATTCT GAGGGGCCCT
    GAAGGTTTCA CCACGCACCT GGAGACCACC TTTACAGGTG ATGGGACCCC CAAGACCATC
    CGGGTGACAC AGCCCCTGGG TCCCCCCACC AAAGCCGTGG ATCTGTCCCA CCAGCCACCG
    GCCGGCAAAG AACAGCCCCT GGCAGTGGAT GGGGCCTCGG GTCCCGGGAA TGGGCCTCAG
    CATGCCTACG ATGATGGGCA GGAGGCTGGC TCACTCCCCC ATGCCAACGG CCTGGCCCCC
    AGGCCCCCAG GCCAGGACCC CGCGAAGAAA GCAACCAGAG TCAGCCTCCA AGGCAGAGGG
    GAGAACAATG AACTGCTCAA GGAGATAGAG CCTGTGCTGA GCCTTCTCAC CAGTGGGAGC
    AGAGGGGTCA AGGGAGGGGC ACCTGCCAAG GCAGAGATGA AAGATATGGG AATCCAGGTG
    GACAGAGATT TGGACGGCAA GTCACACAAA CCTCTGCCCC TCGGCGTGGA GAACGACCGA
    GTCTTCAATG ACCTATGGGG GAAGGGCAAT GTGCCTGTCG TCCTCAACAA CCCATATTCA
    GAGAAGGAGC AGCCCCCCAC CTCAGGAAAA CAGTCCCCCA CAAAGAATGG CAGCCCCTCC
    AAGTGTCCAC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG AGGTGGTTCT CACTGACACC
    CTCCACCTTA AGAGCACATT GGAAACGGGA TGCACTGAGT ACATCTGCAT GGGCTCCATC
    ATGCATCCTT CTCAGCATGC AAGGAGGCCT GAAGACGTCC GCACAAAAGG ACAGCTCTTC
    CCTCTCGCCA AAGAGTTTAT TGATCAATAC TATTCATCAA TTAAAAGATT TGGCTCCAAA
    GCCCACATGG AAAGGCTGGA AGAGGTGAAC AAAGAGATCG ACACCACTAG CACTTACCAG
    CTCAAGGACA CAGAGCTCAT CTATGGGGCC AAGCACGCCT GGCGGAATGC CTCGCGCTGT
    GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTATTCGATG CCCGTGACTG CACCACGGCC
    CACGGGATGT TCAACTACAT CTGTAACCAT GTCAAGTATG CCACCAACAA AGGGAACCTC
    AGGTCTGCCA TCACCATATT CCCCCAGAGG ACAGACGGCA AGCACGACTT CCGAGTCTGG
    AACTCCCAGC TCATCCGCTA CGCTGGCTAC AAGCAGCCTG ACGGCTCCAC CCTGGGGGAC
    CCAGCCAATG TGCAGTTCAC AGAGATATGC ATACAGCAGG GCTGGAAACC GCCTAGAGGC
    CGCTTCGATG TCCTGCCGCT CCTGCTTCAG GCCAACGGCA ATGACCCTGA GCTCTTCCAG
    ATTCCTCCAG AGCTGGTGTT GGAAGTTCCC ATCAGGCACC CCAAGTTTGA GTGGTTCAAG
    GACCTGGGGC TGAAGTGGTA CGGCCTCCCC GCCGTGTCCA ACATGCTCCT AGAGATTGGC
    GGCCTGGAGT TCAGCGCCTG TCCCTTCAGT GGCTGGTACA TGGGCACAGA GATTGGTGTC
    CGCGACTACT GTGACAACTC CCGCTACAAT ATCCTGGAGG AAGTGGCCAA GAAGATGAAC
    TTAGACATGA GGAAGACGTC CTCCCTGTGG AAGGACCAGG CGCTGGTGGA GATCAATATC
    GCGGTTCTCT ATAGCTTCCA GAGTGACAAA GTGACCATTG TTGACCATCA CTCCGCCACC
    GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGGGGCTG CCCTGCCGAC
    TGGGTGTGGA TCGTGCCCCC CATGTCCGGA AGCATCACCC CTGTGTTCCA CCAGGAGATG
    CTCAACTACC GGCTCACCCC CTCCTTCGAA TACCAGCCTG ATCCCTGGAA CACGCATGTC
    TGGAAAGGCA CCAACGGGAC CCCCACAAAG CGGCGAGCCA TTGGCTTCAA GAAGCTAGCA
    GAAGCTGTCA AGTTCTCGGC CAAGCTGATG GGGCAGGCTA TGGCCAAGAG GGTGAAAGCG
    ACCATCCTCT ATGCCACAGA GACAGGCAAA TCGCAAGCTT ATGCCAAGAC CTTGTGTGAG
    ATCTTCAAAC ACGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATTGTGCAC
    CTGGAACATG AAACTCTGGT CCTTGTGGTC ACCAGCACCT TTGGCAATGG AGATCCCCCT
    GAGAATGGGG AGAAATTCGG CTGTGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAG
    GAAGAAAGGA AGAGCTACAA GGTCCGATTC AACAGCGTCT CCTCCTACTC TGACTCCCAA
    AAATCATCAG GCGATGGGCC CGACCTCAGA GACAACTTTG AGAGTGCTGG ACCCCTGGCC
    AATGTGAGGT TCTCAGTTTT TGGCCTCGGC TCACGAGCAT ACCCTCACTT TTGCGCCTTC
    GGACACGCTG TGGACACCCT CCTGGAAGAA CTGGGAGGGG AGAGGATCCT GAAGATGAGG
    GAAGGGGATG AGCTCTGTGG GCAGGAAGAG GCTTTCAGGA CCTGGGCCAA GAAGGTCTTC
    AAGGCAGCCT GTGATGTCTT CTGTGTGGGA GATGATGTCA ACATTGAAAA GGCCAACAAT
    TCCCTCATCA GCAATGATCG CAGCTGGAAG AGAAACAAGT TCCGCCTCAC CTTTGTGGCC
    GAAGCTCCAG AACTCACACA AGGTCTATCC AATGTCCACA AAAAGCGAGT CTCAGCTGCC
    CGGCTCCTTA GCCGTCAAAA CCTCCAGAGC CCTAAATCCA GTCGGTCAAC TATCTTCGTG
    CGTCTCCACA CCAACGGGAG CCAGGAGCTG CAGTACCAGC CTGGGGACCA CCTGGGTGTC
    TTCCCTGGCA ACCACGAGGA CCTCGTGAAT GCCCTGATCG AGCGGCTGGA GGACGCGCCG
    CCTGTCAACC AGATGGTGAA AGTGGAACTG CTGGAGGAGC GGAACACGGC TTTAGGTGTC
    ATCAGTAACT GGACAGACGA GCTCCGCCTC CCGCCCTGCA CCATCTTCCA GGCCTTCAAG
    TACTACCTGG ACATCACCAC GCCACCAACG CCTCTGCAGC TGCAGCAGTT TGCCTCCCTA
    GCTACCAGCG AGAAGGAGAA GCAGCGTCTG CTGGTCCTCA GCAAGGGTTT GCAGGAGTAC
    GAGGAATGGA AATGGGGCAA GAACCCCACC ATCGTGGAGG TGCTGGAGGA GTTCCCATCT
    ATCCAGATGC CGGCCACCCT GCTCCTGACC CAGCTGTCCC TGCTGCAGCC CCGCTACTAT
    TCCATCAGCT CCTCCCCAGA CATGTACCCT GATGAAGTGC ACCTCACTGT GGCCATCGTT
    TCCTACCGCA CTCGAGATGG AGAAGGACCA ATTCACCACG GCGTATGCTC CTCCTGGCTC
    AACCGGATAC AGGCTGACGA ACTGGTCCCC TGTTTCGTGA GAGGAGCACC CAGCTTCCAC
    CTGCCCCGGA ACCCCCAAGT CCCCTGCATC CTCGTTGGAC CAGGCACCGG CATTGCCCCT
    TTCCGAAGCT TCTGGCAACA GCGGCAATTT GATATCCAAC ACAAAGGAAT GAACCCCTGC
    CCCATGGTCC TGGTCTTCGG GTGCCGGCAA TCCAAGATAG ATCATATCTA CAGGGAAGAG
    ACCCTGCAGG CCAAGAACAA GGGGGTCTTC AGAGAGCTGT ACACGGCTTA CTCCCGGGAG
    CCAGACAAAC CAAAGAAGTA CGTGCAGGAC ATCCTGCAGG AGCAGCTGGC GGAGTCTGTG
    TACCGAGCCC TGAAGGAGCA AGGGGGCCAC ATATACGTCT GTGGGGACGT CACCATGGCT
    GCTGATGTCC TCAAAGCCAT CCAGCGCATC ATGACCCAGC AGGGGAAGCT CTCGGCAGAG
    GACGCCGGCG TATTCATCAG CCGGATGAGG GATGACAACC GATACCATGA GGATATTTTT
    GGAGTCACCC TGCGAACGTA CGAAGTGACC AACCGCCTTA GATCTGAGTC CATTGCCTTC
    ATTGAAGAGA GCAAAAAAGA CACCGATGAG GTTTTCAGCT CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000611.1
    CDS712..5016
    Translation

    Target ORF information:

    RefSeq Version NM_000620.4
    Organism Homo sapiens(human)
    Definition Homo sapiens nitric oxide synthase 1 (NOS1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_000620.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAGGATC ACATGTTCGG TGTTCAGCAA ATCCAGCCCA ATGTCATTTC TGTTCGTCTC 
    TTCAAGCGCA AAGTTGGGGG CCTGGGATTT CTGGTGAAGG AGCGGGTCAG TAAGCCGCCC
    GTGATCATCT CTGACCTGAT TCGTGGGGGC GCCGCAGAGC AGAGTGGCCT CATCCAGGCC
    GGAGACATCA TTCTTGCGGT CAACGGCCGG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC
    CTGGAGGTAC TCAGAGGCAT TGCCTCTGAG ACCCACGTGG TCCTCATTCT GAGGGGCCCT
    GAAGGTTTCA CCACGCACCT GGAGACCACC TTTACAGGTG ATGGGACCCC CAAGACCATC
    CGGGTGACAC AGCCCCTGGG TCCCCCCACC AAAGCCGTGG ATCTGTCCCA CCAGCCACCG
    GCCGGCAAAG AACAGCCCCT GGCAGTGGAT GGGGCCTCGG GTCCCGGGAA TGGGCCTCAG
    CATGCCTACG ATGATGGGCA GGAGGCTGGC TCACTCCCCC ATGCCAACGG CCTGGCCCCC
    AGGCCCCCAG GCCAGGACCC CGCGAAGAAA GCAACCAGAG TCAGCCTCCA AGGCAGAGGG
    GAGAACAATG AACTGCTCAA GGAGATAGAG CCTGTGCTGA GCCTTCTCAC CAGTGGGAGC
    AGAGGGGTCA AGGGAGGGGC ACCTGCCAAG GCAGAGATGA AAGATATGGG AATCCAGGTG
    GACAGAGATT TGGACGGCAA GTCACACAAA CCTCTGCCCC TCGGCGTGGA GAACGACCGA
    GTCTTCAATG ACCTATGGGG GAAGGGCAAT GTGCCTGTCG TCCTCAACAA CCCATATTCA
    GAGAAGGAGC AGCCCCCCAC CTCAGGAAAA CAGTCCCCCA CAAAGAATGG CAGCCCCTCC
    AAGTGTCCAC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG AGGTGGTTCT CACTGACACC
    CTCCACCTTA AGAGCACATT GGAAACGGGA TGCACTGAGT ACATCTGCAT GGGCTCCATC
    ATGCATCCTT CTCAGCATGC AAGGAGGCCT GAAGACGTCC GCACAAAAGG ACAGCTCTTC
    CCTCTCGCCA AAGAGTTTAT TGATCAATAC TATTCATCAA TTAAAAGATT TGGCTCCAAA
    GCCCACATGG AAAGGCTGGA AGAGGTGAAC AAAGAGATCG ACACCACTAG CACTTACCAG
    CTCAAGGACA CAGAGCTCAT CTATGGGGCC AAGCACGCCT GGCGGAATGC CTCGCGCTGT
    GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTATTCGATG CCCGTGACTG CACCACGGCC
    CACGGGATGT TCAACTACAT CTGTAACCAT GTCAAGTATG CCACCAACAA AGGGAACCTC
    AGGTCTGCCA TCACCATATT CCCCCAGAGG ACAGACGGCA AGCACGACTT CCGAGTCTGG
    AACTCCCAGC TCATCCGCTA CGCTGGCTAC AAGCAGCCTG ACGGCTCCAC CCTGGGGGAC
    CCAGCCAATG TGCAGTTCAC AGAGATATGC ATACAGCAGG GCTGGAAACC GCCTAGAGGC
    CGCTTCGATG TCCTGCCGCT CCTGCTTCAG GCCAACGGCA ATGACCCTGA GCTCTTCCAG
    ATTCCTCCAG AGCTGGTGTT GGAAGTTCCC ATCAGGCACC CCAAGTTTGA GTGGTTCAAG
    GACCTGGGGC TGAAGTGGTA CGGCCTCCCC GCCGTGTCCA ACATGCTCCT AGAGATTGGC
    GGCCTGGAGT TCAGCGCCTG TCCCTTCAGT GGCTGGTACA TGGGCACAGA GATTGGTGTC
    CGCGACTACT GTGACAACTC CCGCTACAAT ATCCTGGAGG AAGTGGCCAA GAAGATGAAC
    TTAGACATGA GGAAGACGTC CTCCCTGTGG AAGGACCAGG CGCTGGTGGA GATCAATATC
    GCGGTTCTCT ATAGCTTCCA GAGTGACAAA GTGACCATTG TTGACCATCA CTCCGCCACC
    GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGGGGCTG CCCTGCCGAC
    TGGGTGTGGA TCGTGCCCCC CATGTCCGGA AGCATCACCC CTGTGTTCCA CCAGGAGATG
    CTCAACTACC GGCTCACCCC CTCCTTCGAA TACCAGCCTG ATCCCTGGAA CACGCATGTC
    TGGAAAGGCA CCAACGGGAC CCCCACAAAG CGGCGAGCCA TTGGCTTCAA GAAGCTAGCA
    GAAGCTGTCA AGTTCTCGGC CAAGCTGATG GGGCAGGCTA TGGCCAAGAG GGTGAAAGCG
    ACCATCCTCT ATGCCACAGA GACAGGCAAA TCGCAAGCTT ATGCCAAGAC CTTGTGTGAG
    ATCTTCAAAC ACGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATTGTGCAC
    CTGGAACATG AAACTCTGGT CCTTGTGGTC ACCAGCACCT TTGGCAATGG AGATCCCCCT
    GAGAATGGGG AGAAATTCGG CTGTGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAG
    GAAGAAAGGA AGAGCTACAA GGTCCGATTC AACAGCGTCT CCTCCTACTC TGACTCCCAA
    AAATCATCAG GCGATGGGCC CGACCTCAGA GACAACTTTG AGAGTGCTGG ACCCCTGGCC
    AATGTGAGGT TCTCAGTTTT TGGCCTCGGC TCACGAGCAT ACCCTCACTT TTGCGCCTTC
    GGACACGCTG TGGACACCCT CCTGGAAGAA CTGGGAGGGG AGAGGATCCT GAAGATGAGG
    GAAGGGGATG AGCTCTGTGG GCAGGAAGAG GCTTTCAGGA CCTGGGCCAA GAAGGTCTTC
    AAGGCAGCCT GTGATGTCTT CTGTGTGGGA GATGATGTCA ACATTGAAAA GGCCAACAAT
    TCCCTCATCA GCAATGATCG CAGCTGGAAG AGAAACAAGT TCCGCCTCAC CTTTGTGGCC
    GAAGCTCCAG AACTCACACA AGGTCTATCC AATGTCCACA AAAAGCGAGT CTCAGCTGCC
    CGGCTCCTTA GCCGTCAAAA CCTCCAGAGC CCTAAATCCA GTCGGTCAAC TATCTTCGTG
    CGTCTCCACA CCAACGGGAG CCAGGAGCTG CAGTACCAGC CTGGGGACCA CCTGGGTGTC
    TTCCCTGGCA ACCACGAGGA CCTCGTGAAT GCCCTGATCG AGCGGCTGGA GGACGCGCCG
    CCTGTCAACC AGATGGTGAA AGTGGAACTG CTGGAGGAGC GGAACACGGC TTTAGGTGTC
    ATCAGTAACT GGACAGACGA GCTCCGCCTC CCGCCCTGCA CCATCTTCCA GGCCTTCAAG
    TACTACCTGG ACATCACCAC GCCACCAACG CCTCTGCAGC TGCAGCAGTT TGCCTCCCTA
    GCTACCAGCG AGAAGGAGAA GCAGCGTCTG CTGGTCCTCA GCAAGGGTTT GCAGGAGTAC
    GAGGAATGGA AATGGGGCAA GAACCCCACC ATCGTGGAGG TGCTGGAGGA GTTCCCATCT
    ATCCAGATGC CGGCCACCCT GCTCCTGACC CAGCTGTCCC TGCTGCAGCC CCGCTACTAT
    TCCATCAGCT CCTCCCCAGA CATGTACCCT GATGAAGTGC ACCTCACTGT GGCCATCGTT
    TCCTACCGCA CTCGAGATGG AGAAGGACCA ATTCACCACG GCGTATGCTC CTCCTGGCTC
    AACCGGATAC AGGCTGACGA ACTGGTCCCC TGTTTCGTGA GAGGAGCACC CAGCTTCCAC
    CTGCCCCGGA ACCCCCAAGT CCCCTGCATC CTCGTTGGAC CAGGCACCGG CATTGCCCCT
    TTCCGAAGCT TCTGGCAACA GCGGCAATTT GATATCCAAC ACAAAGGAAT GAACCCCTGC
    CCCATGGTCC TGGTCTTCGG GTGCCGGCAA TCCAAGATAG ATCATATCTA CAGGGAAGAG
    ACCCTGCAGG CCAAGAACAA GGGGGTCTTC AGAGAGCTGT ACACGGCTTA CTCCCGGGAG
    CCAGACAAAC CAAAGAAGTA CGTGCAGGAC ATCCTGCAGG AGCAGCTGGC GGAGTCTGTG
    TACCGAGCCC TGAAGGAGCA AGGGGGCCAC ATATACGTCT GTGGGGACGT CACCATGGCT
    GCTGATGTCC TCAAAGCCAT CCAGCGCATC ATGACCCAGC AGGGGAAGCT CTCGGCAGAG
    GACGCCGGCG TATTCATCAG CCGGATGAGG GATGACAACC GATACCATGA GGATATTTTT
    GGAGTCACCC TGCGAACGTA CGAAGTGACC AACCGCCTTA GATCTGAGTC CATTGCCTTC
    ATTGAAGAGA GCAAAAAAGA CACCGATGAG GTTTTCAGCT CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu21020
    Clone ID Related Accession (Same CDS sequence) NM_000620.4 , NM_000620.5 , XM_017019347.1
    Accession Version NM_000620.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4305bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-09
    Organism Homo sapiens(human)
    Product nitric oxide synthase, brain isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U17327.1, AK294435.1, AK307481.1, BC033208.1, AC026364.36 and BE207961.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_000620.4. Transcript Variant: This variant (1) represents the predominant transcript, and encodes isoform 1 (also known as nNOS). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU753241.1, U17327.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03465402, SAMN03465403 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000317775.11/ ENSP00000320758.6 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAGGATC ACATGTTCGG TGTTCAGCAA ATCCAGCCCA ATGTCATTTC TGTTCGTCTC 
    TTCAAGCGCA AAGTTGGGGG CCTGGGATTT CTGGTGAAGG AGCGGGTCAG TAAGCCGCCC
    GTGATCATCT CTGACCTGAT TCGTGGGGGC GCCGCAGAGC AGAGTGGCCT CATCCAGGCC
    GGAGACATCA TTCTTGCGGT CAACGGCCGG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC
    CTGGAGGTAC TCAGAGGCAT TGCCTCTGAG ACCCACGTGG TCCTCATTCT GAGGGGCCCT
    GAAGGTTTCA CCACGCACCT GGAGACCACC TTTACAGGTG ATGGGACCCC CAAGACCATC
    CGGGTGACAC AGCCCCTGGG TCCCCCCACC AAAGCCGTGG ATCTGTCCCA CCAGCCACCG
    GCCGGCAAAG AACAGCCCCT GGCAGTGGAT GGGGCCTCGG GTCCCGGGAA TGGGCCTCAG
    CATGCCTACG ATGATGGGCA GGAGGCTGGC TCACTCCCCC ATGCCAACGG CCTGGCCCCC
    AGGCCCCCAG GCCAGGACCC CGCGAAGAAA GCAACCAGAG TCAGCCTCCA AGGCAGAGGG
    GAGAACAATG AACTGCTCAA GGAGATAGAG CCTGTGCTGA GCCTTCTCAC CAGTGGGAGC
    AGAGGGGTCA AGGGAGGGGC ACCTGCCAAG GCAGAGATGA AAGATATGGG AATCCAGGTG
    GACAGAGATT TGGACGGCAA GTCACACAAA CCTCTGCCCC TCGGCGTGGA GAACGACCGA
    GTCTTCAATG ACCTATGGGG GAAGGGCAAT GTGCCTGTCG TCCTCAACAA CCCATATTCA
    GAGAAGGAGC AGCCCCCCAC CTCAGGAAAA CAGTCCCCCA CAAAGAATGG CAGCCCCTCC
    AAGTGTCCAC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG AGGTGGTTCT CACTGACACC
    CTCCACCTTA AGAGCACATT GGAAACGGGA TGCACTGAGT ACATCTGCAT GGGCTCCATC
    ATGCATCCTT CTCAGCATGC AAGGAGGCCT GAAGACGTCC GCACAAAAGG ACAGCTCTTC
    CCTCTCGCCA AAGAGTTTAT TGATCAATAC TATTCATCAA TTAAAAGATT TGGCTCCAAA
    GCCCACATGG AAAGGCTGGA AGAGGTGAAC AAAGAGATCG ACACCACTAG CACTTACCAG
    CTCAAGGACA CAGAGCTCAT CTATGGGGCC AAGCACGCCT GGCGGAATGC CTCGCGCTGT
    GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTATTCGATG CCCGTGACTG CACCACGGCC
    CACGGGATGT TCAACTACAT CTGTAACCAT GTCAAGTATG CCACCAACAA AGGGAACCTC
    AGGTCTGCCA TCACCATATT CCCCCAGAGG ACAGACGGCA AGCACGACTT CCGAGTCTGG
    AACTCCCAGC TCATCCGCTA CGCTGGCTAC AAGCAGCCTG ACGGCTCCAC CCTGGGGGAC
    CCAGCCAATG TGCAGTTCAC AGAGATATGC ATACAGCAGG GCTGGAAACC GCCTAGAGGC
    CGCTTCGATG TCCTGCCGCT CCTGCTTCAG GCCAACGGCA ATGACCCTGA GCTCTTCCAG
    ATTCCTCCAG AGCTGGTGTT GGAAGTTCCC ATCAGGCACC CCAAGTTTGA GTGGTTCAAG
    GACCTGGGGC TGAAGTGGTA CGGCCTCCCC GCCGTGTCCA ACATGCTCCT AGAGATTGGC
    GGCCTGGAGT TCAGCGCCTG TCCCTTCAGT GGCTGGTACA TGGGCACAGA GATTGGTGTC
    CGCGACTACT GTGACAACTC CCGCTACAAT ATCCTGGAGG AAGTGGCCAA GAAGATGAAC
    TTAGACATGA GGAAGACGTC CTCCCTGTGG AAGGACCAGG CGCTGGTGGA GATCAATATC
    GCGGTTCTCT ATAGCTTCCA GAGTGACAAA GTGACCATTG TTGACCATCA CTCCGCCACC
    GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGGGGCTG CCCTGCCGAC
    TGGGTGTGGA TCGTGCCCCC CATGTCCGGA AGCATCACCC CTGTGTTCCA CCAGGAGATG
    CTCAACTACC GGCTCACCCC CTCCTTCGAA TACCAGCCTG ATCCCTGGAA CACGCATGTC
    TGGAAAGGCA CCAACGGGAC CCCCACAAAG CGGCGAGCCA TTGGCTTCAA GAAGCTAGCA
    GAAGCTGTCA AGTTCTCGGC CAAGCTGATG GGGCAGGCTA TGGCCAAGAG GGTGAAAGCG
    ACCATCCTCT ATGCCACAGA GACAGGCAAA TCGCAAGCTT ATGCCAAGAC CTTGTGTGAG
    ATCTTCAAAC ACGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATTGTGCAC
    CTGGAACATG AAACTCTGGT CCTTGTGGTC ACCAGCACCT TTGGCAATGG AGATCCCCCT
    GAGAATGGGG AGAAATTCGG CTGTGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAG
    GAAGAAAGGA AGAGCTACAA GGTCCGATTC AACAGCGTCT CCTCCTACTC TGACTCCCAA
    AAATCATCAG GCGATGGGCC CGACCTCAGA GACAACTTTG AGAGTGCTGG ACCCCTGGCC
    AATGTGAGGT TCTCAGTTTT TGGCCTCGGC TCACGAGCAT ACCCTCACTT TTGCGCCTTC
    GGACACGCTG TGGACACCCT CCTGGAAGAA CTGGGAGGGG AGAGGATCCT GAAGATGAGG
    GAAGGGGATG AGCTCTGTGG GCAGGAAGAG GCTTTCAGGA CCTGGGCCAA GAAGGTCTTC
    AAGGCAGCCT GTGATGTCTT CTGTGTGGGA GATGATGTCA ACATTGAAAA GGCCAACAAT
    TCCCTCATCA GCAATGATCG CAGCTGGAAG AGAAACAAGT TCCGCCTCAC CTTTGTGGCC
    GAAGCTCCAG AACTCACACA AGGTCTATCC AATGTCCACA AAAAGCGAGT CTCAGCTGCC
    CGGCTCCTTA GCCGTCAAAA CCTCCAGAGC CCTAAATCCA GTCGGTCAAC TATCTTCGTG
    CGTCTCCACA CCAACGGGAG CCAGGAGCTG CAGTACCAGC CTGGGGACCA CCTGGGTGTC
    TTCCCTGGCA ACCACGAGGA CCTCGTGAAT GCCCTGATCG AGCGGCTGGA GGACGCGCCG
    CCTGTCAACC AGATGGTGAA AGTGGAACTG CTGGAGGAGC GGAACACGGC TTTAGGTGTC
    ATCAGTAACT GGACAGACGA GCTCCGCCTC CCGCCCTGCA CCATCTTCCA GGCCTTCAAG
    TACTACCTGG ACATCACCAC GCCACCAACG CCTCTGCAGC TGCAGCAGTT TGCCTCCCTA
    GCTACCAGCG AGAAGGAGAA GCAGCGTCTG CTGGTCCTCA GCAAGGGTTT GCAGGAGTAC
    GAGGAATGGA AATGGGGCAA GAACCCCACC ATCGTGGAGG TGCTGGAGGA GTTCCCATCT
    ATCCAGATGC CGGCCACCCT GCTCCTGACC CAGCTGTCCC TGCTGCAGCC CCGCTACTAT
    TCCATCAGCT CCTCCCCAGA CATGTACCCT GATGAAGTGC ACCTCACTGT GGCCATCGTT
    TCCTACCGCA CTCGAGATGG AGAAGGACCA ATTCACCACG GCGTATGCTC CTCCTGGCTC
    AACCGGATAC AGGCTGACGA ACTGGTCCCC TGTTTCGTGA GAGGAGCACC CAGCTTCCAC
    CTGCCCCGGA ACCCCCAAGT CCCCTGCATC CTCGTTGGAC CAGGCACCGG CATTGCCCCT
    TTCCGAAGCT TCTGGCAACA GCGGCAATTT GATATCCAAC ACAAAGGAAT GAACCCCTGC
    CCCATGGTCC TGGTCTTCGG GTGCCGGCAA TCCAAGATAG ATCATATCTA CAGGGAAGAG
    ACCCTGCAGG CCAAGAACAA GGGGGTCTTC AGAGAGCTGT ACACGGCTTA CTCCCGGGAG
    CCAGACAAAC CAAAGAAGTA CGTGCAGGAC ATCCTGCAGG AGCAGCTGGC GGAGTCTGTG
    TACCGAGCCC TGAAGGAGCA AGGGGGCCAC ATATACGTCT GTGGGGACGT CACCATGGCT
    GCTGATGTCC TCAAAGCCAT CCAGCGCATC ATGACCCAGC AGGGGAAGCT CTCGGCAGAG
    GACGCCGGCG TATTCATCAG CCGGATGAGG GATGACAACC GATACCATGA GGATATTTTT
    GGAGTCACCC TGCGAACGTA CGAAGTGACC AACCGCCTTA GATCTGAGTC CATTGCCTTC
    ATTGAAGAGA GCAAAAAAGA CACCGATGAG GTTTTCAGCT CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000611.1
    CDS536..4840
    Translation

    Target ORF information:

    RefSeq Version NM_000620.5
    Organism Homo sapiens(human)
    Definition Homo sapiens nitric oxide synthase 1 (NOS1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_000620.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAGGATC ACATGTTCGG TGTTCAGCAA ATCCAGCCCA ATGTCATTTC TGTTCGTCTC 
    TTCAAGCGCA AAGTTGGGGG CCTGGGATTT CTGGTGAAGG AGCGGGTCAG TAAGCCGCCC
    GTGATCATCT CTGACCTGAT TCGTGGGGGC GCCGCAGAGC AGAGTGGCCT CATCCAGGCC
    GGAGACATCA TTCTTGCGGT CAACGGCCGG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC
    CTGGAGGTAC TCAGAGGCAT TGCCTCTGAG ACCCACGTGG TCCTCATTCT GAGGGGCCCT
    GAAGGTTTCA CCACGCACCT GGAGACCACC TTTACAGGTG ATGGGACCCC CAAGACCATC
    CGGGTGACAC AGCCCCTGGG TCCCCCCACC AAAGCCGTGG ATCTGTCCCA CCAGCCACCG
    GCCGGCAAAG AACAGCCCCT GGCAGTGGAT GGGGCCTCGG GTCCCGGGAA TGGGCCTCAG
    CATGCCTACG ATGATGGGCA GGAGGCTGGC TCACTCCCCC ATGCCAACGG CCTGGCCCCC
    AGGCCCCCAG GCCAGGACCC CGCGAAGAAA GCAACCAGAG TCAGCCTCCA AGGCAGAGGG
    GAGAACAATG AACTGCTCAA GGAGATAGAG CCTGTGCTGA GCCTTCTCAC CAGTGGGAGC
    AGAGGGGTCA AGGGAGGGGC ACCTGCCAAG GCAGAGATGA AAGATATGGG AATCCAGGTG
    GACAGAGATT TGGACGGCAA GTCACACAAA CCTCTGCCCC TCGGCGTGGA GAACGACCGA
    GTCTTCAATG ACCTATGGGG GAAGGGCAAT GTGCCTGTCG TCCTCAACAA CCCATATTCA
    GAGAAGGAGC AGCCCCCCAC CTCAGGAAAA CAGTCCCCCA CAAAGAATGG CAGCCCCTCC
    AAGTGTCCAC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG AGGTGGTTCT CACTGACACC
    CTCCACCTTA AGAGCACATT GGAAACGGGA TGCACTGAGT ACATCTGCAT GGGCTCCATC
    ATGCATCCTT CTCAGCATGC AAGGAGGCCT GAAGACGTCC GCACAAAAGG ACAGCTCTTC
    CCTCTCGCCA AAGAGTTTAT TGATCAATAC TATTCATCAA TTAAAAGATT TGGCTCCAAA
    GCCCACATGG AAAGGCTGGA AGAGGTGAAC AAAGAGATCG ACACCACTAG CACTTACCAG
    CTCAAGGACA CAGAGCTCAT CTATGGGGCC AAGCACGCCT GGCGGAATGC CTCGCGCTGT
    GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTATTCGATG CCCGTGACTG CACCACGGCC
    CACGGGATGT TCAACTACAT CTGTAACCAT GTCAAGTATG CCACCAACAA AGGGAACCTC
    AGGTCTGCCA TCACCATATT CCCCCAGAGG ACAGACGGCA AGCACGACTT CCGAGTCTGG
    AACTCCCAGC TCATCCGCTA CGCTGGCTAC AAGCAGCCTG ACGGCTCCAC CCTGGGGGAC
    CCAGCCAATG TGCAGTTCAC AGAGATATGC ATACAGCAGG GCTGGAAACC GCCTAGAGGC
    CGCTTCGATG TCCTGCCGCT CCTGCTTCAG GCCAACGGCA ATGACCCTGA GCTCTTCCAG
    ATTCCTCCAG AGCTGGTGTT GGAAGTTCCC ATCAGGCACC CCAAGTTTGA GTGGTTCAAG
    GACCTGGGGC TGAAGTGGTA CGGCCTCCCC GCCGTGTCCA ACATGCTCCT AGAGATTGGC
    GGCCTGGAGT TCAGCGCCTG TCCCTTCAGT GGCTGGTACA TGGGCACAGA GATTGGTGTC
    CGCGACTACT GTGACAACTC CCGCTACAAT ATCCTGGAGG AAGTGGCCAA GAAGATGAAC
    TTAGACATGA GGAAGACGTC CTCCCTGTGG AAGGACCAGG CGCTGGTGGA GATCAATATC
    GCGGTTCTCT ATAGCTTCCA GAGTGACAAA GTGACCATTG TTGACCATCA CTCCGCCACC
    GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGGGGCTG CCCTGCCGAC
    TGGGTGTGGA TCGTGCCCCC CATGTCCGGA AGCATCACCC CTGTGTTCCA CCAGGAGATG
    CTCAACTACC GGCTCACCCC CTCCTTCGAA TACCAGCCTG ATCCCTGGAA CACGCATGTC
    TGGAAAGGCA CCAACGGGAC CCCCACAAAG CGGCGAGCCA TTGGCTTCAA GAAGCTAGCA
    GAAGCTGTCA AGTTCTCGGC CAAGCTGATG GGGCAGGCTA TGGCCAAGAG GGTGAAAGCG
    ACCATCCTCT ATGCCACAGA GACAGGCAAA TCGCAAGCTT ATGCCAAGAC CTTGTGTGAG
    ATCTTCAAAC ACGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATTGTGCAC
    CTGGAACATG AAACTCTGGT CCTTGTGGTC ACCAGCACCT TTGGCAATGG AGATCCCCCT
    GAGAATGGGG AGAAATTCGG CTGTGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAG
    GAAGAAAGGA AGAGCTACAA GGTCCGATTC AACAGCGTCT CCTCCTACTC TGACTCCCAA
    AAATCATCAG GCGATGGGCC CGACCTCAGA GACAACTTTG AGAGTGCTGG ACCCCTGGCC
    AATGTGAGGT TCTCAGTTTT TGGCCTCGGC TCACGAGCAT ACCCTCACTT TTGCGCCTTC
    GGACACGCTG TGGACACCCT CCTGGAAGAA CTGGGAGGGG AGAGGATCCT GAAGATGAGG
    GAAGGGGATG AGCTCTGTGG GCAGGAAGAG GCTTTCAGGA CCTGGGCCAA GAAGGTCTTC
    AAGGCAGCCT GTGATGTCTT CTGTGTGGGA GATGATGTCA ACATTGAAAA GGCCAACAAT
    TCCCTCATCA GCAATGATCG CAGCTGGAAG AGAAACAAGT TCCGCCTCAC CTTTGTGGCC
    GAAGCTCCAG AACTCACACA AGGTCTATCC AATGTCCACA AAAAGCGAGT CTCAGCTGCC
    CGGCTCCTTA GCCGTCAAAA CCTCCAGAGC CCTAAATCCA GTCGGTCAAC TATCTTCGTG
    CGTCTCCACA CCAACGGGAG CCAGGAGCTG CAGTACCAGC CTGGGGACCA CCTGGGTGTC
    TTCCCTGGCA ACCACGAGGA CCTCGTGAAT GCCCTGATCG AGCGGCTGGA GGACGCGCCG
    CCTGTCAACC AGATGGTGAA AGTGGAACTG CTGGAGGAGC GGAACACGGC TTTAGGTGTC
    ATCAGTAACT GGACAGACGA GCTCCGCCTC CCGCCCTGCA CCATCTTCCA GGCCTTCAAG
    TACTACCTGG ACATCACCAC GCCACCAACG CCTCTGCAGC TGCAGCAGTT TGCCTCCCTA
    GCTACCAGCG AGAAGGAGAA GCAGCGTCTG CTGGTCCTCA GCAAGGGTTT GCAGGAGTAC
    GAGGAATGGA AATGGGGCAA GAACCCCACC ATCGTGGAGG TGCTGGAGGA GTTCCCATCT
    ATCCAGATGC CGGCCACCCT GCTCCTGACC CAGCTGTCCC TGCTGCAGCC CCGCTACTAT
    TCCATCAGCT CCTCCCCAGA CATGTACCCT GATGAAGTGC ACCTCACTGT GGCCATCGTT
    TCCTACCGCA CTCGAGATGG AGAAGGACCA ATTCACCACG GCGTATGCTC CTCCTGGCTC
    AACCGGATAC AGGCTGACGA ACTGGTCCCC TGTTTCGTGA GAGGAGCACC CAGCTTCCAC
    CTGCCCCGGA ACCCCCAAGT CCCCTGCATC CTCGTTGGAC CAGGCACCGG CATTGCCCCT
    TTCCGAAGCT TCTGGCAACA GCGGCAATTT GATATCCAAC ACAAAGGAAT GAACCCCTGC
    CCCATGGTCC TGGTCTTCGG GTGCCGGCAA TCCAAGATAG ATCATATCTA CAGGGAAGAG
    ACCCTGCAGG CCAAGAACAA GGGGGTCTTC AGAGAGCTGT ACACGGCTTA CTCCCGGGAG
    CCAGACAAAC CAAAGAAGTA CGTGCAGGAC ATCCTGCAGG AGCAGCTGGC GGAGTCTGTG
    TACCGAGCCC TGAAGGAGCA AGGGGGCCAC ATATACGTCT GTGGGGACGT CACCATGGCT
    GCTGATGTCC TCAAAGCCAT CCAGCGCATC ATGACCCAGC AGGGGAAGCT CTCGGCAGAG
    GACGCCGGCG TATTCATCAG CCGGATGAGG GATGACAACC GATACCATGA GGATATTTTT
    GGAGTCACCC TGCGAACGTA CGAAGTGACC AACCGCCTTA GATCTGAGTC CATTGCCTTC
    ATTGAAGAGA GCAAAAAAGA CACCGATGAG GTTTTCAGCT CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu21540
    Clone ID Related Accession (Same CDS sequence) XM_017019345.1 , XM_011538398.2 , XM_017019346.1 , NM_001204218.1
    Accession Version NM_001204218.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4407bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-09
    Organism Homo sapiens(human)
    Product nitric oxide synthase, brain isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK307481.1, U17327.1, AK294435.1, AJ004918.1, BC033208.1, AC026364.36 and BE207961.1. Transcript Variant: This variant (2) contains an additional in-frame coding exon compared to variant 1, resulting in a longer isoform (2, also known as nNOSmu) containing a 34 aa protein segment not found in isoform 1. A similar isoform has also been reported in rat and mouse, and the mouse isoform has been shown to have catalytic activity (PMID:9791007). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAGGATC ACATGTTCGG TGTTCAGCAA ATCCAGCCCA ATGTCATTTC TGTTCGTCTC 
    TTCAAGCGCA AAGTTGGGGG CCTGGGATTT CTGGTGAAGG AGCGGGTCAG TAAGCCGCCC
    GTGATCATCT CTGACCTGAT TCGTGGGGGC GCCGCAGAGC AGAGTGGCCT CATCCAGGCC
    GGAGACATCA TTCTTGCGGT CAACGGCCGG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC
    CTGGAGGTAC TCAGAGGCAT TGCCTCTGAG ACCCACGTGG TCCTCATTCT GAGGGGCCCT
    GAAGGTTTCA CCACGCACCT GGAGACCACC TTTACAGGTG ATGGGACCCC CAAGACCATC
    CGGGTGACAC AGCCCCTGGG TCCCCCCACC AAAGCCGTGG ATCTGTCCCA CCAGCCACCG
    GCCGGCAAAG AACAGCCCCT GGCAGTGGAT GGGGCCTCGG GTCCCGGGAA TGGGCCTCAG
    CATGCCTACG ATGATGGGCA GGAGGCTGGC TCACTCCCCC ATGCCAACGG CCTGGCCCCC
    AGGCCCCCAG GCCAGGACCC CGCGAAGAAA GCAACCAGAG TCAGCCTCCA AGGCAGAGGG
    GAGAACAATG AACTGCTCAA GGAGATAGAG CCTGTGCTGA GCCTTCTCAC CAGTGGGAGC
    AGAGGGGTCA AGGGAGGGGC ACCTGCCAAG GCAGAGATGA AAGATATGGG AATCCAGGTG
    GACAGAGATT TGGACGGCAA GTCACACAAA CCTCTGCCCC TCGGCGTGGA GAACGACCGA
    GTCTTCAATG ACCTATGGGG GAAGGGCAAT GTGCCTGTCG TCCTCAACAA CCCATATTCA
    GAGAAGGAGC AGCCCCCCAC CTCAGGAAAA CAGTCCCCCA CAAAGAATGG CAGCCCCTCC
    AAGTGTCCAC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG AGGTGGTTCT CACTGACACC
    CTCCACCTTA AGAGCACATT GGAAACGGGA TGCACTGAGT ACATCTGCAT GGGCTCCATC
    ATGCATCCTT CTCAGCATGC AAGGAGGCCT GAAGACGTCC GCACAAAAGG ACAGCTCTTC
    CCTCTCGCCA AAGAGTTTAT TGATCAATAC TATTCATCAA TTAAAAGATT TGGCTCCAAA
    GCCCACATGG AAAGGCTGGA AGAGGTGAAC AAAGAGATCG ACACCACTAG CACTTACCAG
    CTCAAGGACA CAGAGCTCAT CTATGGGGCC AAGCACGCCT GGCGGAATGC CTCGCGCTGT
    GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTATTCGATG CCCGTGACTG CACCACGGCC
    CACGGGATGT TCAACTACAT CTGTAACCAT GTCAAGTATG CCACCAACAA AGGGAACCTC
    AGGTCTGCCA TCACCATATT CCCCCAGAGG ACAGACGGCA AGCACGACTT CCGAGTCTGG
    AACTCCCAGC TCATCCGCTA CGCTGGCTAC AAGCAGCCTG ACGGCTCCAC CCTGGGGGAC
    CCAGCCAATG TGCAGTTCAC AGAGATATGC ATACAGCAGG GCTGGAAACC GCCTAGAGGC
    CGCTTCGATG TCCTGCCGCT CCTGCTTCAG GCCAACGGCA ATGACCCTGA GCTCTTCCAG
    ATTCCTCCAG AGCTGGTGTT GGAAGTTCCC ATCAGGCACC CCAAGTTTGA GTGGTTCAAG
    GACCTGGGGC TGAAGTGGTA CGGCCTCCCC GCCGTGTCCA ACATGCTCCT AGAGATTGGC
    GGCCTGGAGT TCAGCGCCTG TCCCTTCAGT GGCTGGTACA TGGGCACAGA GATTGGTGTC
    CGCGACTACT GTGACAACTC CCGCTACAAT ATCCTGGAGG AAGTGGCCAA GAAGATGAAC
    TTAGACATGA GGAAGACGTC CTCCCTGTGG AAGGACCAGG CGCTGGTGGA GATCAATATC
    GCGGTTCTCT ATAGCTTCCA GAGTGACAAA GTGACCATTG TTGACCATCA CTCCGCCACC
    GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGGGGCTG CCCTGCCGAC
    TGGGTGTGGA TCGTGCCCCC CATGTCCGGA AGCATCACCC CTGTGTTCCA CCAGGAGATG
    CTCAACTACC GGCTCACCCC CTCCTTCGAA TACCAGCCTG ATCCCTGGAA CACGCATGTC
    TGGAAAGGCA CCAACGGGAC CCCCACAAAG CGGCGAGCCA TTGGCTTCAA GAAGCTAGCA
    GAAGCTGTCA AGTTCTCGGC CAAGCTGATG GGGCAGGCTA TGGCCAAGAG GGTGAAAGCG
    ACCATCCTCT ATGCCACAGA GACAGGCAAA TCGCAAGCTT ATGCCAAGAC CTTGTGTGAG
    ATCTTCAAAC ACGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATTGTGCAC
    CTGGAACATG AAACTCTGGT CCTTGTGGTC ACCAGCACCT TTGGCAATGG AGATCCCCCT
    GAGAATGGGG AGAAATTCGG CTGTGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAG
    GAAGAAAGGA AGTACCCGGA ACCCTTGCGT TTCTTTCCCC GTAAAGGGCC TCCCCTCCCC
    AATGGTGACA CAGAAGTCCA CGGTCTGGCT GCAGCCCGTG ACAGCCAGCA CAGGAGCTAC
    AAGGTCCGAT TCAACAGCGT CTCCTCCTAC TCTGACTCCC AAAAATCATC AGGCGATGGG
    CCCGACCTCA GAGACAACTT TGAGAGTGCT GGACCCCTGG CCAATGTGAG GTTCTCAGTT
    TTTGGCCTCG GCTCACGAGC ATACCCTCAC TTTTGCGCCT TCGGACACGC TGTGGACACC
    CTCCTGGAAG AACTGGGAGG GGAGAGGATC CTGAAGATGA GGGAAGGGGA TGAGCTCTGT
    GGGCAGGAAG AGGCTTTCAG GACCTGGGCC AAGAAGGTCT TCAAGGCAGC CTGTGATGTC
    TTCTGTGTGG GAGATGATGT CAACATTGAA AAGGCCAACA ATTCCCTCAT CAGCAATGAT
    CGCAGCTGGA AGAGAAACAA GTTCCGCCTC ACCTTTGTGG CCGAAGCTCC AGAACTCACA
    CAAGGTCTAT CCAATGTCCA CAAAAAGCGA GTCTCAGCTG CCCGGCTCCT TAGCCGTCAA
    AACCTCCAGA GCCCTAAATC CAGTCGGTCA ACTATCTTCG TGCGTCTCCA CACCAACGGG
    AGCCAGGAGC TGCAGTACCA GCCTGGGGAC CACCTGGGTG TCTTCCCTGG CAACCACGAG
    GACCTCGTGA ATGCCCTGAT CGAGCGGCTG GAGGACGCGC CGCCTGTCAA CCAGATGGTG
    AAAGTGGAAC TGCTGGAGGA GCGGAACACG GCTTTAGGTG TCATCAGTAA CTGGACAGAC
    GAGCTCCGCC TCCCGCCCTG CACCATCTTC CAGGCCTTCA AGTACTACCT GGACATCACC
    ACGCCACCAA CGCCTCTGCA GCTGCAGCAG TTTGCCTCCC TAGCTACCAG CGAGAAGGAG
    AAGCAGCGTC TGCTGGTCCT CAGCAAGGGT TTGCAGGAGT ACGAGGAATG GAAATGGGGC
    AAGAACCCCA CCATCGTGGA GGTGCTGGAG GAGTTCCCAT CTATCCAGAT GCCGGCCACC
    CTGCTCCTGA CCCAGCTGTC CCTGCTGCAG CCCCGCTACT ATTCCATCAG CTCCTCCCCA
    GACATGTACC CTGATGAAGT GCACCTCACT GTGGCCATCG TTTCCTACCG CACTCGAGAT
    GGAGAAGGAC CAATTCACCA CGGCGTATGC TCCTCCTGGC TCAACCGGAT ACAGGCTGAC
    GAACTGGTCC CCTGTTTCGT GAGAGGAGCA CCCAGCTTCC ACCTGCCCCG GAACCCCCAA
    GTCCCCTGCA TCCTCGTTGG ACCAGGCACC GGCATTGCCC CTTTCCGAAG CTTCTGGCAA
    CAGCGGCAAT TTGATATCCA ACACAAAGGA ATGAACCCCT GCCCCATGGT CCTGGTCTTC
    GGGTGCCGGC AATCCAAGAT AGATCATATC TACAGGGAAG AGACCCTGCA GGCCAAGAAC
    AAGGGGGTCT TCAGAGAGCT GTACACGGCT TACTCCCGGG AGCCAGACAA ACCAAAGAAG
    TACGTGCAGG ACATCCTGCA GGAGCAGCTG GCGGAGTCTG TGTACCGAGC CCTGAAGGAG
    CAAGGGGGCC ACATATACGT CTGTGGGGAC GTCACCATGG CTGCTGATGT CCTCAAAGCC
    ATCCAGCGCA TCATGACCCA GCAGGGGAAG CTCTCGGCAG AGGACGCCGG CGTATTCATC
    AGCCGGATGA GGGATGACAA CCGATACCAT GAGGATATTT TTGGAGTCAC CCTGCGAACG
    TACGAAGTGA CCAACCGCCT TAGATCTGAG TCCATTGCCT TCATTGAAGA GAGCAAAAAA
    GACACCGATG AGGTTTTCAG CTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001191147.1
    CDS712..5118
    Translation

    Target ORF information:

    RefSeq Version NM_001204218.1
    Organism Homo sapiens(human)
    Definition Homo sapiens nitric oxide synthase 1 (NOS1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001204218.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAGGATC ACATGTTCGG TGTTCAGCAA ATCCAGCCCA ATGTCATTTC TGTTCGTCTC 
    TTCAAGCGCA AAGTTGGGGG CCTGGGATTT CTGGTGAAGG AGCGGGTCAG TAAGCCGCCC
    GTGATCATCT CTGACCTGAT TCGTGGGGGC GCCGCAGAGC AGAGTGGCCT CATCCAGGCC
    GGAGACATCA TTCTTGCGGT CAACGGCCGG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC
    CTGGAGGTAC TCAGAGGCAT TGCCTCTGAG ACCCACGTGG TCCTCATTCT GAGGGGCCCT
    GAAGGTTTCA CCACGCACCT GGAGACCACC TTTACAGGTG ATGGGACCCC CAAGACCATC
    CGGGTGACAC AGCCCCTGGG TCCCCCCACC AAAGCCGTGG ATCTGTCCCA CCAGCCACCG
    GCCGGCAAAG AACAGCCCCT GGCAGTGGAT GGGGCCTCGG GTCCCGGGAA TGGGCCTCAG
    CATGCCTACG ATGATGGGCA GGAGGCTGGC TCACTCCCCC ATGCCAACGG CCTGGCCCCC
    AGGCCCCCAG GCCAGGACCC CGCGAAGAAA GCAACCAGAG TCAGCCTCCA AGGCAGAGGG
    GAGAACAATG AACTGCTCAA GGAGATAGAG CCTGTGCTGA GCCTTCTCAC CAGTGGGAGC
    AGAGGGGTCA AGGGAGGGGC ACCTGCCAAG GCAGAGATGA AAGATATGGG AATCCAGGTG
    GACAGAGATT TGGACGGCAA GTCACACAAA CCTCTGCCCC TCGGCGTGGA GAACGACCGA
    GTCTTCAATG ACCTATGGGG GAAGGGCAAT GTGCCTGTCG TCCTCAACAA CCCATATTCA
    GAGAAGGAGC AGCCCCCCAC CTCAGGAAAA CAGTCCCCCA CAAAGAATGG CAGCCCCTCC
    AAGTGTCCAC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG AGGTGGTTCT CACTGACACC
    CTCCACCTTA AGAGCACATT GGAAACGGGA TGCACTGAGT ACATCTGCAT GGGCTCCATC
    ATGCATCCTT CTCAGCATGC AAGGAGGCCT GAAGACGTCC GCACAAAAGG ACAGCTCTTC
    CCTCTCGCCA AAGAGTTTAT TGATCAATAC TATTCATCAA TTAAAAGATT TGGCTCCAAA
    GCCCACATGG AAAGGCTGGA AGAGGTGAAC AAAGAGATCG ACACCACTAG CACTTACCAG
    CTCAAGGACA CAGAGCTCAT CTATGGGGCC AAGCACGCCT GGCGGAATGC CTCGCGCTGT
    GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTATTCGATG CCCGTGACTG CACCACGGCC
    CACGGGATGT TCAACTACAT CTGTAACCAT GTCAAGTATG CCACCAACAA AGGGAACCTC
    AGGTCTGCCA TCACCATATT CCCCCAGAGG ACAGACGGCA AGCACGACTT CCGAGTCTGG
    AACTCCCAGC TCATCCGCTA CGCTGGCTAC AAGCAGCCTG ACGGCTCCAC CCTGGGGGAC
    CCAGCCAATG TGCAGTTCAC AGAGATATGC ATACAGCAGG GCTGGAAACC GCCTAGAGGC
    CGCTTCGATG TCCTGCCGCT CCTGCTTCAG GCCAACGGCA ATGACCCTGA GCTCTTCCAG
    ATTCCTCCAG AGCTGGTGTT GGAAGTTCCC ATCAGGCACC CCAAGTTTGA GTGGTTCAAG
    GACCTGGGGC TGAAGTGGTA CGGCCTCCCC GCCGTGTCCA ACATGCTCCT AGAGATTGGC
    GGCCTGGAGT TCAGCGCCTG TCCCTTCAGT GGCTGGTACA TGGGCACAGA GATTGGTGTC
    CGCGACTACT GTGACAACTC CCGCTACAAT ATCCTGGAGG AAGTGGCCAA GAAGATGAAC
    TTAGACATGA GGAAGACGTC CTCCCTGTGG AAGGACCAGG CGCTGGTGGA GATCAATATC
    GCGGTTCTCT ATAGCTTCCA GAGTGACAAA GTGACCATTG TTGACCATCA CTCCGCCACC
    GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGGGGCTG CCCTGCCGAC
    TGGGTGTGGA TCGTGCCCCC CATGTCCGGA AGCATCACCC CTGTGTTCCA CCAGGAGATG
    CTCAACTACC GGCTCACCCC CTCCTTCGAA TACCAGCCTG ATCCCTGGAA CACGCATGTC
    TGGAAAGGCA CCAACGGGAC CCCCACAAAG CGGCGAGCCA TTGGCTTCAA GAAGCTAGCA
    GAAGCTGTCA AGTTCTCGGC CAAGCTGATG GGGCAGGCTA TGGCCAAGAG GGTGAAAGCG
    ACCATCCTCT ATGCCACAGA GACAGGCAAA TCGCAAGCTT ATGCCAAGAC CTTGTGTGAG
    ATCTTCAAAC ACGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATTGTGCAC
    CTGGAACATG AAACTCTGGT CCTTGTGGTC ACCAGCACCT TTGGCAATGG AGATCCCCCT
    GAGAATGGGG AGAAATTCGG CTGTGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAG
    GAAGAAAGGA AGTACCCGGA ACCCTTGCGT TTCTTTCCCC GTAAAGGGCC TCCCCTCCCC
    AATGGTGACA CAGAAGTCCA CGGTCTGGCT GCAGCCCGTG ACAGCCAGCA CAGGAGCTAC
    AAGGTCCGAT TCAACAGCGT CTCCTCCTAC TCTGACTCCC AAAAATCATC AGGCGATGGG
    CCCGACCTCA GAGACAACTT TGAGAGTGCT GGACCCCTGG CCAATGTGAG GTTCTCAGTT
    TTTGGCCTCG GCTCACGAGC ATACCCTCAC TTTTGCGCCT TCGGACACGC TGTGGACACC
    CTCCTGGAAG AACTGGGAGG GGAGAGGATC CTGAAGATGA GGGAAGGGGA TGAGCTCTGT
    GGGCAGGAAG AGGCTTTCAG GACCTGGGCC AAGAAGGTCT TCAAGGCAGC CTGTGATGTC
    TTCTGTGTGG GAGATGATGT CAACATTGAA AAGGCCAACA ATTCCCTCAT CAGCAATGAT
    CGCAGCTGGA AGAGAAACAA GTTCCGCCTC ACCTTTGTGG CCGAAGCTCC AGAACTCACA
    CAAGGTCTAT CCAATGTCCA CAAAAAGCGA GTCTCAGCTG CCCGGCTCCT TAGCCGTCAA
    AACCTCCAGA GCCCTAAATC CAGTCGGTCA ACTATCTTCG TGCGTCTCCA CACCAACGGG
    AGCCAGGAGC TGCAGTACCA GCCTGGGGAC CACCTGGGTG TCTTCCCTGG CAACCACGAG
    GACCTCGTGA ATGCCCTGAT CGAGCGGCTG GAGGACGCGC CGCCTGTCAA CCAGATGGTG
    AAAGTGGAAC TGCTGGAGGA GCGGAACACG GCTTTAGGTG TCATCAGTAA CTGGACAGAC
    GAGCTCCGCC TCCCGCCCTG CACCATCTTC CAGGCCTTCA AGTACTACCT GGACATCACC
    ACGCCACCAA CGCCTCTGCA GCTGCAGCAG TTTGCCTCCC TAGCTACCAG CGAGAAGGAG
    AAGCAGCGTC TGCTGGTCCT CAGCAAGGGT TTGCAGGAGT ACGAGGAATG GAAATGGGGC
    AAGAACCCCA CCATCGTGGA GGTGCTGGAG GAGTTCCCAT CTATCCAGAT GCCGGCCACC
    CTGCTCCTGA CCCAGCTGTC CCTGCTGCAG CCCCGCTACT ATTCCATCAG CTCCTCCCCA
    GACATGTACC CTGATGAAGT GCACCTCACT GTGGCCATCG TTTCCTACCG CACTCGAGAT
    GGAGAAGGAC CAATTCACCA CGGCGTATGC TCCTCCTGGC TCAACCGGAT ACAGGCTGAC
    GAACTGGTCC CCTGTTTCGT GAGAGGAGCA CCCAGCTTCC ACCTGCCCCG GAACCCCCAA
    GTCCCCTGCA TCCTCGTTGG ACCAGGCACC GGCATTGCCC CTTTCCGAAG CTTCTGGCAA
    CAGCGGCAAT TTGATATCCA ACACAAAGGA ATGAACCCCT GCCCCATGGT CCTGGTCTTC
    GGGTGCCGGC AATCCAAGAT AGATCATATC TACAGGGAAG AGACCCTGCA GGCCAAGAAC
    AAGGGGGTCT TCAGAGAGCT GTACACGGCT TACTCCCGGG AGCCAGACAA ACCAAAGAAG
    TACGTGCAGG ACATCCTGCA GGAGCAGCTG GCGGAGTCTG TGTACCGAGC CCTGAAGGAG
    CAAGGGGGCC ACATATACGT CTGTGGGGAC GTCACCATGG CTGCTGATGT CCTCAAAGCC
    ATCCAGCGCA TCATGACCCA GCAGGGGAAG CTCTCGGCAG AGGACGCCGG CGTATTCATC
    AGCCGGATGA GGGATGACAA CCGATACCAT GAGGATATTT TTGGAGTCAC CCTGCGAACG
    TACGAAGTGA CCAACCGCCT TAGATCTGAG TCCATTGCCT TCATTGAAGA GAGCAAAAAA
    GACACCGATG AGGTTTTCAG CTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu21020
    Clone ID Related Accession (Same CDS sequence) NM_000620.4 , NM_000620.5 , XM_017019347.1
    Accession Version XM_017019347.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4305bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product nitric oxide synthase, brain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAGGATC ACATGTTCGG TGTTCAGCAA ATCCAGCCCA ATGTCATTTC TGTTCGTCTC 
    TTCAAGCGCA AAGTTGGGGG CCTGGGATTT CTGGTGAAGG AGCGGGTCAG TAAGCCGCCC
    GTGATCATCT CTGACCTGAT TCGTGGGGGC GCCGCAGAGC AGAGTGGCCT CATCCAGGCC
    GGAGACATCA TTCTTGCGGT CAACGGCCGG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC
    CTGGAGGTAC TCAGAGGCAT TGCCTCTGAG ACCCACGTGG TCCTCATTCT GAGGGGCCCT
    GAAGGTTTCA CCACGCACCT GGAGACCACC TTTACAGGTG ATGGGACCCC CAAGACCATC
    CGGGTGACAC AGCCCCTGGG TCCCCCCACC AAAGCCGTGG ATCTGTCCCA CCAGCCACCG
    GCCGGCAAAG AACAGCCCCT GGCAGTGGAT GGGGCCTCGG GTCCCGGGAA TGGGCCTCAG
    CATGCCTACG ATGATGGGCA GGAGGCTGGC TCACTCCCCC ATGCCAACGG CCTGGCCCCC
    AGGCCCCCAG GCCAGGACCC CGCGAAGAAA GCAACCAGAG TCAGCCTCCA AGGCAGAGGG
    GAGAACAATG AACTGCTCAA GGAGATAGAG CCTGTGCTGA GCCTTCTCAC CAGTGGGAGC
    AGAGGGGTCA AGGGAGGGGC ACCTGCCAAG GCAGAGATGA AAGATATGGG AATCCAGGTG
    GACAGAGATT TGGACGGCAA GTCACACAAA CCTCTGCCCC TCGGCGTGGA GAACGACCGA
    GTCTTCAATG ACCTATGGGG GAAGGGCAAT GTGCCTGTCG TCCTCAACAA CCCATATTCA
    GAGAAGGAGC AGCCCCCCAC CTCAGGAAAA CAGTCCCCCA CAAAGAATGG CAGCCCCTCC
    AAGTGTCCAC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG AGGTGGTTCT CACTGACACC
    CTCCACCTTA AGAGCACATT GGAAACGGGA TGCACTGAGT ACATCTGCAT GGGCTCCATC
    ATGCATCCTT CTCAGCATGC AAGGAGGCCT GAAGACGTCC GCACAAAAGG ACAGCTCTTC
    CCTCTCGCCA AAGAGTTTAT TGATCAATAC TATTCATCAA TTAAAAGATT TGGCTCCAAA
    GCCCACATGG AAAGGCTGGA AGAGGTGAAC AAAGAGATCG ACACCACTAG CACTTACCAG
    CTCAAGGACA CAGAGCTCAT CTATGGGGCC AAGCACGCCT GGCGGAATGC CTCGCGCTGT
    GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTATTCGATG CCCGTGACTG CACCACGGCC
    CACGGGATGT TCAACTACAT CTGTAACCAT GTCAAGTATG CCACCAACAA AGGGAACCTC
    AGGTCTGCCA TCACCATATT CCCCCAGAGG ACAGACGGCA AGCACGACTT CCGAGTCTGG
    AACTCCCAGC TCATCCGCTA CGCTGGCTAC AAGCAGCCTG ACGGCTCCAC CCTGGGGGAC
    CCAGCCAATG TGCAGTTCAC AGAGATATGC ATACAGCAGG GCTGGAAACC GCCTAGAGGC
    CGCTTCGATG TCCTGCCGCT CCTGCTTCAG GCCAACGGCA ATGACCCTGA GCTCTTCCAG
    ATTCCTCCAG AGCTGGTGTT GGAAGTTCCC ATCAGGCACC CCAAGTTTGA GTGGTTCAAG
    GACCTGGGGC TGAAGTGGTA CGGCCTCCCC GCCGTGTCCA ACATGCTCCT AGAGATTGGC
    GGCCTGGAGT TCAGCGCCTG TCCCTTCAGT GGCTGGTACA TGGGCACAGA GATTGGTGTC
    CGCGACTACT GTGACAACTC CCGCTACAAT ATCCTGGAGG AAGTGGCCAA GAAGATGAAC
    TTAGACATGA GGAAGACGTC CTCCCTGTGG AAGGACCAGG CGCTGGTGGA GATCAATATC
    GCGGTTCTCT ATAGCTTCCA GAGTGACAAA GTGACCATTG TTGACCATCA CTCCGCCACC
    GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGGGGCTG CCCTGCCGAC
    TGGGTGTGGA TCGTGCCCCC CATGTCCGGA AGCATCACCC CTGTGTTCCA CCAGGAGATG
    CTCAACTACC GGCTCACCCC CTCCTTCGAA TACCAGCCTG ATCCCTGGAA CACGCATGTC
    TGGAAAGGCA CCAACGGGAC CCCCACAAAG CGGCGAGCCA TTGGCTTCAA GAAGCTAGCA
    GAAGCTGTCA AGTTCTCGGC CAAGCTGATG GGGCAGGCTA TGGCCAAGAG GGTGAAAGCG
    ACCATCCTCT ATGCCACAGA GACAGGCAAA TCGCAAGCTT ATGCCAAGAC CTTGTGTGAG
    ATCTTCAAAC ACGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATTGTGCAC
    CTGGAACATG AAACTCTGGT CCTTGTGGTC ACCAGCACCT TTGGCAATGG AGATCCCCCT
    GAGAATGGGG AGAAATTCGG CTGTGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAG
    GAAGAAAGGA AGAGCTACAA GGTCCGATTC AACAGCGTCT CCTCCTACTC TGACTCCCAA
    AAATCATCAG GCGATGGGCC CGACCTCAGA GACAACTTTG AGAGTGCTGG ACCCCTGGCC
    AATGTGAGGT TCTCAGTTTT TGGCCTCGGC TCACGAGCAT ACCCTCACTT TTGCGCCTTC
    GGACACGCTG TGGACACCCT CCTGGAAGAA CTGGGAGGGG AGAGGATCCT GAAGATGAGG
    GAAGGGGATG AGCTCTGTGG GCAGGAAGAG GCTTTCAGGA CCTGGGCCAA GAAGGTCTTC
    AAGGCAGCCT GTGATGTCTT CTGTGTGGGA GATGATGTCA ACATTGAAAA GGCCAACAAT
    TCCCTCATCA GCAATGATCG CAGCTGGAAG AGAAACAAGT TCCGCCTCAC CTTTGTGGCC
    GAAGCTCCAG AACTCACACA AGGTCTATCC AATGTCCACA AAAAGCGAGT CTCAGCTGCC
    CGGCTCCTTA GCCGTCAAAA CCTCCAGAGC CCTAAATCCA GTCGGTCAAC TATCTTCGTG
    CGTCTCCACA CCAACGGGAG CCAGGAGCTG CAGTACCAGC CTGGGGACCA CCTGGGTGTC
    TTCCCTGGCA ACCACGAGGA CCTCGTGAAT GCCCTGATCG AGCGGCTGGA GGACGCGCCG
    CCTGTCAACC AGATGGTGAA AGTGGAACTG CTGGAGGAGC GGAACACGGC TTTAGGTGTC
    ATCAGTAACT GGACAGACGA GCTCCGCCTC CCGCCCTGCA CCATCTTCCA GGCCTTCAAG
    TACTACCTGG ACATCACCAC GCCACCAACG CCTCTGCAGC TGCAGCAGTT TGCCTCCCTA
    GCTACCAGCG AGAAGGAGAA GCAGCGTCTG CTGGTCCTCA GCAAGGGTTT GCAGGAGTAC
    GAGGAATGGA AATGGGGCAA GAACCCCACC ATCGTGGAGG TGCTGGAGGA GTTCCCATCT
    ATCCAGATGC CGGCCACCCT GCTCCTGACC CAGCTGTCCC TGCTGCAGCC CCGCTACTAT
    TCCATCAGCT CCTCCCCAGA CATGTACCCT GATGAAGTGC ACCTCACTGT GGCCATCGTT
    TCCTACCGCA CTCGAGATGG AGAAGGACCA ATTCACCACG GCGTATGCTC CTCCTGGCTC
    AACCGGATAC AGGCTGACGA ACTGGTCCCC TGTTTCGTGA GAGGAGCACC CAGCTTCCAC
    CTGCCCCGGA ACCCCCAAGT CCCCTGCATC CTCGTTGGAC CAGGCACCGG CATTGCCCCT
    TTCCGAAGCT TCTGGCAACA GCGGCAATTT GATATCCAAC ACAAAGGAAT GAACCCCTGC
    CCCATGGTCC TGGTCTTCGG GTGCCGGCAA TCCAAGATAG ATCATATCTA CAGGGAAGAG
    ACCCTGCAGG CCAAGAACAA GGGGGTCTTC AGAGAGCTGT ACACGGCTTA CTCCCGGGAG
    CCAGACAAAC CAAAGAAGTA CGTGCAGGAC ATCCTGCAGG AGCAGCTGGC GGAGTCTGTG
    TACCGAGCCC TGAAGGAGCA AGGGGGCCAC ATATACGTCT GTGGGGACGT CACCATGGCT
    GCTGATGTCC TCAAAGCCAT CCAGCGCATC ATGACCCAGC AGGGGAAGCT CTCGGCAGAG
    GACGCCGGCG TATTCATCAG CCGGATGAGG GATGACAACC GATACCATGA GGATATTTTT
    GGAGTCACCC TGCGAACGTA CGAAGTGACC AACCGCCTTA GATCTGAGTC CATTGCCTTC
    ATTGAAGAGA GCAAAAAAGA CACCGATGAG GTTTTCAGCT CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016874836.1
    CDS622..4926
    Translation

    Target ORF information:

    RefSeq Version XM_017019347.1
    Organism Homo sapiens(human)
    Definition Homo sapiens nitric oxide synthase 1 (NOS1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017019347.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAGGATC ACATGTTCGG TGTTCAGCAA ATCCAGCCCA ATGTCATTTC TGTTCGTCTC 
    TTCAAGCGCA AAGTTGGGGG CCTGGGATTT CTGGTGAAGG AGCGGGTCAG TAAGCCGCCC
    GTGATCATCT CTGACCTGAT TCGTGGGGGC GCCGCAGAGC AGAGTGGCCT CATCCAGGCC
    GGAGACATCA TTCTTGCGGT CAACGGCCGG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC
    CTGGAGGTAC TCAGAGGCAT TGCCTCTGAG ACCCACGTGG TCCTCATTCT GAGGGGCCCT
    GAAGGTTTCA CCACGCACCT GGAGACCACC TTTACAGGTG ATGGGACCCC CAAGACCATC
    CGGGTGACAC AGCCCCTGGG TCCCCCCACC AAAGCCGTGG ATCTGTCCCA CCAGCCACCG
    GCCGGCAAAG AACAGCCCCT GGCAGTGGAT GGGGCCTCGG GTCCCGGGAA TGGGCCTCAG
    CATGCCTACG ATGATGGGCA GGAGGCTGGC TCACTCCCCC ATGCCAACGG CCTGGCCCCC
    AGGCCCCCAG GCCAGGACCC CGCGAAGAAA GCAACCAGAG TCAGCCTCCA AGGCAGAGGG
    GAGAACAATG AACTGCTCAA GGAGATAGAG CCTGTGCTGA GCCTTCTCAC CAGTGGGAGC
    AGAGGGGTCA AGGGAGGGGC ACCTGCCAAG GCAGAGATGA AAGATATGGG AATCCAGGTG
    GACAGAGATT TGGACGGCAA GTCACACAAA CCTCTGCCCC TCGGCGTGGA GAACGACCGA
    GTCTTCAATG ACCTATGGGG GAAGGGCAAT GTGCCTGTCG TCCTCAACAA CCCATATTCA
    GAGAAGGAGC AGCCCCCCAC CTCAGGAAAA CAGTCCCCCA CAAAGAATGG CAGCCCCTCC
    AAGTGTCCAC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG AGGTGGTTCT CACTGACACC
    CTCCACCTTA AGAGCACATT GGAAACGGGA TGCACTGAGT ACATCTGCAT GGGCTCCATC
    ATGCATCCTT CTCAGCATGC AAGGAGGCCT GAAGACGTCC GCACAAAAGG ACAGCTCTTC
    CCTCTCGCCA AAGAGTTTAT TGATCAATAC TATTCATCAA TTAAAAGATT TGGCTCCAAA
    GCCCACATGG AAAGGCTGGA AGAGGTGAAC AAAGAGATCG ACACCACTAG CACTTACCAG
    CTCAAGGACA CAGAGCTCAT CTATGGGGCC AAGCACGCCT GGCGGAATGC CTCGCGCTGT
    GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTATTCGATG CCCGTGACTG CACCACGGCC
    CACGGGATGT TCAACTACAT CTGTAACCAT GTCAAGTATG CCACCAACAA AGGGAACCTC
    AGGTCTGCCA TCACCATATT CCCCCAGAGG ACAGACGGCA AGCACGACTT CCGAGTCTGG
    AACTCCCAGC TCATCCGCTA CGCTGGCTAC AAGCAGCCTG ACGGCTCCAC CCTGGGGGAC
    CCAGCCAATG TGCAGTTCAC AGAGATATGC ATACAGCAGG GCTGGAAACC GCCTAGAGGC
    CGCTTCGATG TCCTGCCGCT CCTGCTTCAG GCCAACGGCA ATGACCCTGA GCTCTTCCAG
    ATTCCTCCAG AGCTGGTGTT GGAAGTTCCC ATCAGGCACC CCAAGTTTGA GTGGTTCAAG
    GACCTGGGGC TGAAGTGGTA CGGCCTCCCC GCCGTGTCCA ACATGCTCCT AGAGATTGGC
    GGCCTGGAGT TCAGCGCCTG TCCCTTCAGT GGCTGGTACA TGGGCACAGA GATTGGTGTC
    CGCGACTACT GTGACAACTC CCGCTACAAT ATCCTGGAGG AAGTGGCCAA GAAGATGAAC
    TTAGACATGA GGAAGACGTC CTCCCTGTGG AAGGACCAGG CGCTGGTGGA GATCAATATC
    GCGGTTCTCT ATAGCTTCCA GAGTGACAAA GTGACCATTG TTGACCATCA CTCCGCCACC
    GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGGGGCTG CCCTGCCGAC
    TGGGTGTGGA TCGTGCCCCC CATGTCCGGA AGCATCACCC CTGTGTTCCA CCAGGAGATG
    CTCAACTACC GGCTCACCCC CTCCTTCGAA TACCAGCCTG ATCCCTGGAA CACGCATGTC
    TGGAAAGGCA CCAACGGGAC CCCCACAAAG CGGCGAGCCA TTGGCTTCAA GAAGCTAGCA
    GAAGCTGTCA AGTTCTCGGC CAAGCTGATG GGGCAGGCTA TGGCCAAGAG GGTGAAAGCG
    ACCATCCTCT ATGCCACAGA GACAGGCAAA TCGCAAGCTT ATGCCAAGAC CTTGTGTGAG
    ATCTTCAAAC ACGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATTGTGCAC
    CTGGAACATG AAACTCTGGT CCTTGTGGTC ACCAGCACCT TTGGCAATGG AGATCCCCCT
    GAGAATGGGG AGAAATTCGG CTGTGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAG
    GAAGAAAGGA AGAGCTACAA GGTCCGATTC AACAGCGTCT CCTCCTACTC TGACTCCCAA
    AAATCATCAG GCGATGGGCC CGACCTCAGA GACAACTTTG AGAGTGCTGG ACCCCTGGCC
    AATGTGAGGT TCTCAGTTTT TGGCCTCGGC TCACGAGCAT ACCCTCACTT TTGCGCCTTC
    GGACACGCTG TGGACACCCT CCTGGAAGAA CTGGGAGGGG AGAGGATCCT GAAGATGAGG
    GAAGGGGATG AGCTCTGTGG GCAGGAAGAG GCTTTCAGGA CCTGGGCCAA GAAGGTCTTC
    AAGGCAGCCT GTGATGTCTT CTGTGTGGGA GATGATGTCA ACATTGAAAA GGCCAACAAT
    TCCCTCATCA GCAATGATCG CAGCTGGAAG AGAAACAAGT TCCGCCTCAC CTTTGTGGCC
    GAAGCTCCAG AACTCACACA AGGTCTATCC AATGTCCACA AAAAGCGAGT CTCAGCTGCC
    CGGCTCCTTA GCCGTCAAAA CCTCCAGAGC CCTAAATCCA GTCGGTCAAC TATCTTCGTG
    CGTCTCCACA CCAACGGGAG CCAGGAGCTG CAGTACCAGC CTGGGGACCA CCTGGGTGTC
    TTCCCTGGCA ACCACGAGGA CCTCGTGAAT GCCCTGATCG AGCGGCTGGA GGACGCGCCG
    CCTGTCAACC AGATGGTGAA AGTGGAACTG CTGGAGGAGC GGAACACGGC TTTAGGTGTC
    ATCAGTAACT GGACAGACGA GCTCCGCCTC CCGCCCTGCA CCATCTTCCA GGCCTTCAAG
    TACTACCTGG ACATCACCAC GCCACCAACG CCTCTGCAGC TGCAGCAGTT TGCCTCCCTA
    GCTACCAGCG AGAAGGAGAA GCAGCGTCTG CTGGTCCTCA GCAAGGGTTT GCAGGAGTAC
    GAGGAATGGA AATGGGGCAA GAACCCCACC ATCGTGGAGG TGCTGGAGGA GTTCCCATCT
    ATCCAGATGC CGGCCACCCT GCTCCTGACC CAGCTGTCCC TGCTGCAGCC CCGCTACTAT
    TCCATCAGCT CCTCCCCAGA CATGTACCCT GATGAAGTGC ACCTCACTGT GGCCATCGTT
    TCCTACCGCA CTCGAGATGG AGAAGGACCA ATTCACCACG GCGTATGCTC CTCCTGGCTC
    AACCGGATAC AGGCTGACGA ACTGGTCCCC TGTTTCGTGA GAGGAGCACC CAGCTTCCAC
    CTGCCCCGGA ACCCCCAAGT CCCCTGCATC CTCGTTGGAC CAGGCACCGG CATTGCCCCT
    TTCCGAAGCT TCTGGCAACA GCGGCAATTT GATATCCAAC ACAAAGGAAT GAACCCCTGC
    CCCATGGTCC TGGTCTTCGG GTGCCGGCAA TCCAAGATAG ATCATATCTA CAGGGAAGAG
    ACCCTGCAGG CCAAGAACAA GGGGGTCTTC AGAGAGCTGT ACACGGCTTA CTCCCGGGAG
    CCAGACAAAC CAAAGAAGTA CGTGCAGGAC ATCCTGCAGG AGCAGCTGGC GGAGTCTGTG
    TACCGAGCCC TGAAGGAGCA AGGGGGCCAC ATATACGTCT GTGGGGACGT CACCATGGCT
    GCTGATGTCC TCAAAGCCAT CCAGCGCATC ATGACCCAGC AGGGGAAGCT CTCGGCAGAG
    GACGCCGGCG TATTCATCAG CCGGATGAGG GATGACAACC GATACCATGA GGATATTTTT
    GGAGTCACCC TGCGAACGTA CGAAGTGACC AACCGCCTTA GATCTGAGTC CATTGCCTTC
    ATTGAAGAGA GCAAAAAAGA CACCGATGAG GTTTTCAGCT CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu21540
    Clone ID Related Accession (Same CDS sequence) XM_017019345.1 , XM_011538398.2 , XM_017019346.1 , NM_001204218.1
    Accession Version XM_017019345.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4407bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product nitric oxide synthase, brain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAGGATC ACATGTTCGG TGTTCAGCAA ATCCAGCCCA ATGTCATTTC TGTTCGTCTC 
    TTCAAGCGCA AAGTTGGGGG CCTGGGATTT CTGGTGAAGG AGCGGGTCAG TAAGCCGCCC
    GTGATCATCT CTGACCTGAT TCGTGGGGGC GCCGCAGAGC AGAGTGGCCT CATCCAGGCC
    GGAGACATCA TTCTTGCGGT CAACGGCCGG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC
    CTGGAGGTAC TCAGAGGCAT TGCCTCTGAG ACCCACGTGG TCCTCATTCT GAGGGGCCCT
    GAAGGTTTCA CCACGCACCT GGAGACCACC TTTACAGGTG ATGGGACCCC CAAGACCATC
    CGGGTGACAC AGCCCCTGGG TCCCCCCACC AAAGCCGTGG ATCTGTCCCA CCAGCCACCG
    GCCGGCAAAG AACAGCCCCT GGCAGTGGAT GGGGCCTCGG GTCCCGGGAA TGGGCCTCAG
    CATGCCTACG ATGATGGGCA GGAGGCTGGC TCACTCCCCC ATGCCAACGG CCTGGCCCCC
    AGGCCCCCAG GCCAGGACCC CGCGAAGAAA GCAACCAGAG TCAGCCTCCA AGGCAGAGGG
    GAGAACAATG AACTGCTCAA GGAGATAGAG CCTGTGCTGA GCCTTCTCAC CAGTGGGAGC
    AGAGGGGTCA AGGGAGGGGC ACCTGCCAAG GCAGAGATGA AAGATATGGG AATCCAGGTG
    GACAGAGATT TGGACGGCAA GTCACACAAA CCTCTGCCCC TCGGCGTGGA GAACGACCGA
    GTCTTCAATG ACCTATGGGG GAAGGGCAAT GTGCCTGTCG TCCTCAACAA CCCATATTCA
    GAGAAGGAGC AGCCCCCCAC CTCAGGAAAA CAGTCCCCCA CAAAGAATGG CAGCCCCTCC
    AAGTGTCCAC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG AGGTGGTTCT CACTGACACC
    CTCCACCTTA AGAGCACATT GGAAACGGGA TGCACTGAGT ACATCTGCAT GGGCTCCATC
    ATGCATCCTT CTCAGCATGC AAGGAGGCCT GAAGACGTCC GCACAAAAGG ACAGCTCTTC
    CCTCTCGCCA AAGAGTTTAT TGATCAATAC TATTCATCAA TTAAAAGATT TGGCTCCAAA
    GCCCACATGG AAAGGCTGGA AGAGGTGAAC AAAGAGATCG ACACCACTAG CACTTACCAG
    CTCAAGGACA CAGAGCTCAT CTATGGGGCC AAGCACGCCT GGCGGAATGC CTCGCGCTGT
    GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTATTCGATG CCCGTGACTG CACCACGGCC
    CACGGGATGT TCAACTACAT CTGTAACCAT GTCAAGTATG CCACCAACAA AGGGAACCTC
    AGGTCTGCCA TCACCATATT CCCCCAGAGG ACAGACGGCA AGCACGACTT CCGAGTCTGG
    AACTCCCAGC TCATCCGCTA CGCTGGCTAC AAGCAGCCTG ACGGCTCCAC CCTGGGGGAC
    CCAGCCAATG TGCAGTTCAC AGAGATATGC ATACAGCAGG GCTGGAAACC GCCTAGAGGC
    CGCTTCGATG TCCTGCCGCT CCTGCTTCAG GCCAACGGCA ATGACCCTGA GCTCTTCCAG
    ATTCCTCCAG AGCTGGTGTT GGAAGTTCCC ATCAGGCACC CCAAGTTTGA GTGGTTCAAG
    GACCTGGGGC TGAAGTGGTA CGGCCTCCCC GCCGTGTCCA ACATGCTCCT AGAGATTGGC
    GGCCTGGAGT TCAGCGCCTG TCCCTTCAGT GGCTGGTACA TGGGCACAGA GATTGGTGTC
    CGCGACTACT GTGACAACTC CCGCTACAAT ATCCTGGAGG AAGTGGCCAA GAAGATGAAC
    TTAGACATGA GGAAGACGTC CTCCCTGTGG AAGGACCAGG CGCTGGTGGA GATCAATATC
    GCGGTTCTCT ATAGCTTCCA GAGTGACAAA GTGACCATTG TTGACCATCA CTCCGCCACC
    GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGGGGCTG CCCTGCCGAC
    TGGGTGTGGA TCGTGCCCCC CATGTCCGGA AGCATCACCC CTGTGTTCCA CCAGGAGATG
    CTCAACTACC GGCTCACCCC CTCCTTCGAA TACCAGCCTG ATCCCTGGAA CACGCATGTC
    TGGAAAGGCA CCAACGGGAC CCCCACAAAG CGGCGAGCCA TTGGCTTCAA GAAGCTAGCA
    GAAGCTGTCA AGTTCTCGGC CAAGCTGATG GGGCAGGCTA TGGCCAAGAG GGTGAAAGCG
    ACCATCCTCT ATGCCACAGA GACAGGCAAA TCGCAAGCTT ATGCCAAGAC CTTGTGTGAG
    ATCTTCAAAC ACGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATTGTGCAC
    CTGGAACATG AAACTCTGGT CCTTGTGGTC ACCAGCACCT TTGGCAATGG AGATCCCCCT
    GAGAATGGGG AGAAATTCGG CTGTGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAG
    GAAGAAAGGA AGTACCCGGA ACCCTTGCGT TTCTTTCCCC GTAAAGGGCC TCCCCTCCCC
    AATGGTGACA CAGAAGTCCA CGGTCTGGCT GCAGCCCGTG ACAGCCAGCA CAGGAGCTAC
    AAGGTCCGAT TCAACAGCGT CTCCTCCTAC TCTGACTCCC AAAAATCATC AGGCGATGGG
    CCCGACCTCA GAGACAACTT TGAGAGTGCT GGACCCCTGG CCAATGTGAG GTTCTCAGTT
    TTTGGCCTCG GCTCACGAGC ATACCCTCAC TTTTGCGCCT TCGGACACGC TGTGGACACC
    CTCCTGGAAG AACTGGGAGG GGAGAGGATC CTGAAGATGA GGGAAGGGGA TGAGCTCTGT
    GGGCAGGAAG AGGCTTTCAG GACCTGGGCC AAGAAGGTCT TCAAGGCAGC CTGTGATGTC
    TTCTGTGTGG GAGATGATGT CAACATTGAA AAGGCCAACA ATTCCCTCAT CAGCAATGAT
    CGCAGCTGGA AGAGAAACAA GTTCCGCCTC ACCTTTGTGG CCGAAGCTCC AGAACTCACA
    CAAGGTCTAT CCAATGTCCA CAAAAAGCGA GTCTCAGCTG CCCGGCTCCT TAGCCGTCAA
    AACCTCCAGA GCCCTAAATC CAGTCGGTCA ACTATCTTCG TGCGTCTCCA CACCAACGGG
    AGCCAGGAGC TGCAGTACCA GCCTGGGGAC CACCTGGGTG TCTTCCCTGG CAACCACGAG
    GACCTCGTGA ATGCCCTGAT CGAGCGGCTG GAGGACGCGC CGCCTGTCAA CCAGATGGTG
    AAAGTGGAAC TGCTGGAGGA GCGGAACACG GCTTTAGGTG TCATCAGTAA CTGGACAGAC
    GAGCTCCGCC TCCCGCCCTG CACCATCTTC CAGGCCTTCA AGTACTACCT GGACATCACC
    ACGCCACCAA CGCCTCTGCA GCTGCAGCAG TTTGCCTCCC TAGCTACCAG CGAGAAGGAG
    AAGCAGCGTC TGCTGGTCCT CAGCAAGGGT TTGCAGGAGT ACGAGGAATG GAAATGGGGC
    AAGAACCCCA CCATCGTGGA GGTGCTGGAG GAGTTCCCAT CTATCCAGAT GCCGGCCACC
    CTGCTCCTGA CCCAGCTGTC CCTGCTGCAG CCCCGCTACT ATTCCATCAG CTCCTCCCCA
    GACATGTACC CTGATGAAGT GCACCTCACT GTGGCCATCG TTTCCTACCG CACTCGAGAT
    GGAGAAGGAC CAATTCACCA CGGCGTATGC TCCTCCTGGC TCAACCGGAT ACAGGCTGAC
    GAACTGGTCC CCTGTTTCGT GAGAGGAGCA CCCAGCTTCC ACCTGCCCCG GAACCCCCAA
    GTCCCCTGCA TCCTCGTTGG ACCAGGCACC GGCATTGCCC CTTTCCGAAG CTTCTGGCAA
    CAGCGGCAAT TTGATATCCA ACACAAAGGA ATGAACCCCT GCCCCATGGT CCTGGTCTTC
    GGGTGCCGGC AATCCAAGAT AGATCATATC TACAGGGAAG AGACCCTGCA GGCCAAGAAC
    AAGGGGGTCT TCAGAGAGCT GTACACGGCT TACTCCCGGG AGCCAGACAA ACCAAAGAAG
    TACGTGCAGG ACATCCTGCA GGAGCAGCTG GCGGAGTCTG TGTACCGAGC CCTGAAGGAG
    CAAGGGGGCC ACATATACGT CTGTGGGGAC GTCACCATGG CTGCTGATGT CCTCAAAGCC
    ATCCAGCGCA TCATGACCCA GCAGGGGAAG CTCTCGGCAG AGGACGCCGG CGTATTCATC
    AGCCGGATGA GGGATGACAA CCGATACCAT GAGGATATTT TTGGAGTCAC CCTGCGAACG
    TACGAAGTGA CCAACCGCCT TAGATCTGAG TCCATTGCCT TCATTGAAGA GAGCAAAAAA
    GACACCGATG AGGTTTTCAG CTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016874834.1
    CDS719..5125
    Translation

    Target ORF information:

    RefSeq Version XM_017019345.1
    Organism Homo sapiens(human)
    Definition Homo sapiens nitric oxide synthase 1 (NOS1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017019345.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAGGATC ACATGTTCGG TGTTCAGCAA ATCCAGCCCA ATGTCATTTC TGTTCGTCTC 
    TTCAAGCGCA AAGTTGGGGG CCTGGGATTT CTGGTGAAGG AGCGGGTCAG TAAGCCGCCC
    GTGATCATCT CTGACCTGAT TCGTGGGGGC GCCGCAGAGC AGAGTGGCCT CATCCAGGCC
    GGAGACATCA TTCTTGCGGT CAACGGCCGG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC
    CTGGAGGTAC TCAGAGGCAT TGCCTCTGAG ACCCACGTGG TCCTCATTCT GAGGGGCCCT
    GAAGGTTTCA CCACGCACCT GGAGACCACC TTTACAGGTG ATGGGACCCC CAAGACCATC
    CGGGTGACAC AGCCCCTGGG TCCCCCCACC AAAGCCGTGG ATCTGTCCCA CCAGCCACCG
    GCCGGCAAAG AACAGCCCCT GGCAGTGGAT GGGGCCTCGG GTCCCGGGAA TGGGCCTCAG
    CATGCCTACG ATGATGGGCA GGAGGCTGGC TCACTCCCCC ATGCCAACGG CCTGGCCCCC
    AGGCCCCCAG GCCAGGACCC CGCGAAGAAA GCAACCAGAG TCAGCCTCCA AGGCAGAGGG
    GAGAACAATG AACTGCTCAA GGAGATAGAG CCTGTGCTGA GCCTTCTCAC CAGTGGGAGC
    AGAGGGGTCA AGGGAGGGGC ACCTGCCAAG GCAGAGATGA AAGATATGGG AATCCAGGTG
    GACAGAGATT TGGACGGCAA GTCACACAAA CCTCTGCCCC TCGGCGTGGA GAACGACCGA
    GTCTTCAATG ACCTATGGGG GAAGGGCAAT GTGCCTGTCG TCCTCAACAA CCCATATTCA
    GAGAAGGAGC AGCCCCCCAC CTCAGGAAAA CAGTCCCCCA CAAAGAATGG CAGCCCCTCC
    AAGTGTCCAC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG AGGTGGTTCT CACTGACACC
    CTCCACCTTA AGAGCACATT GGAAACGGGA TGCACTGAGT ACATCTGCAT GGGCTCCATC
    ATGCATCCTT CTCAGCATGC AAGGAGGCCT GAAGACGTCC GCACAAAAGG ACAGCTCTTC
    CCTCTCGCCA AAGAGTTTAT TGATCAATAC TATTCATCAA TTAAAAGATT TGGCTCCAAA
    GCCCACATGG AAAGGCTGGA AGAGGTGAAC AAAGAGATCG ACACCACTAG CACTTACCAG
    CTCAAGGACA CAGAGCTCAT CTATGGGGCC AAGCACGCCT GGCGGAATGC CTCGCGCTGT
    GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTATTCGATG CCCGTGACTG CACCACGGCC
    CACGGGATGT TCAACTACAT CTGTAACCAT GTCAAGTATG CCACCAACAA AGGGAACCTC
    AGGTCTGCCA TCACCATATT CCCCCAGAGG ACAGACGGCA AGCACGACTT CCGAGTCTGG
    AACTCCCAGC TCATCCGCTA CGCTGGCTAC AAGCAGCCTG ACGGCTCCAC CCTGGGGGAC
    CCAGCCAATG TGCAGTTCAC AGAGATATGC ATACAGCAGG GCTGGAAACC GCCTAGAGGC
    CGCTTCGATG TCCTGCCGCT CCTGCTTCAG GCCAACGGCA ATGACCCTGA GCTCTTCCAG
    ATTCCTCCAG AGCTGGTGTT GGAAGTTCCC ATCAGGCACC CCAAGTTTGA GTGGTTCAAG
    GACCTGGGGC TGAAGTGGTA CGGCCTCCCC GCCGTGTCCA ACATGCTCCT AGAGATTGGC
    GGCCTGGAGT TCAGCGCCTG TCCCTTCAGT GGCTGGTACA TGGGCACAGA GATTGGTGTC
    CGCGACTACT GTGACAACTC CCGCTACAAT ATCCTGGAGG AAGTGGCCAA GAAGATGAAC
    TTAGACATGA GGAAGACGTC CTCCCTGTGG AAGGACCAGG CGCTGGTGGA GATCAATATC
    GCGGTTCTCT ATAGCTTCCA GAGTGACAAA GTGACCATTG TTGACCATCA CTCCGCCACC
    GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGGGGCTG CCCTGCCGAC
    TGGGTGTGGA TCGTGCCCCC CATGTCCGGA AGCATCACCC CTGTGTTCCA CCAGGAGATG
    CTCAACTACC GGCTCACCCC CTCCTTCGAA TACCAGCCTG ATCCCTGGAA CACGCATGTC
    TGGAAAGGCA CCAACGGGAC CCCCACAAAG CGGCGAGCCA TTGGCTTCAA GAAGCTAGCA
    GAAGCTGTCA AGTTCTCGGC CAAGCTGATG GGGCAGGCTA TGGCCAAGAG GGTGAAAGCG
    ACCATCCTCT ATGCCACAGA GACAGGCAAA TCGCAAGCTT ATGCCAAGAC CTTGTGTGAG
    ATCTTCAAAC ACGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATTGTGCAC
    CTGGAACATG AAACTCTGGT CCTTGTGGTC ACCAGCACCT TTGGCAATGG AGATCCCCCT
    GAGAATGGGG AGAAATTCGG CTGTGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAG
    GAAGAAAGGA AGTACCCGGA ACCCTTGCGT TTCTTTCCCC GTAAAGGGCC TCCCCTCCCC
    AATGGTGACA CAGAAGTCCA CGGTCTGGCT GCAGCCCGTG ACAGCCAGCA CAGGAGCTAC
    AAGGTCCGAT TCAACAGCGT CTCCTCCTAC TCTGACTCCC AAAAATCATC AGGCGATGGG
    CCCGACCTCA GAGACAACTT TGAGAGTGCT GGACCCCTGG CCAATGTGAG GTTCTCAGTT
    TTTGGCCTCG GCTCACGAGC ATACCCTCAC TTTTGCGCCT TCGGACACGC TGTGGACACC
    CTCCTGGAAG AACTGGGAGG GGAGAGGATC CTGAAGATGA GGGAAGGGGA TGAGCTCTGT
    GGGCAGGAAG AGGCTTTCAG GACCTGGGCC AAGAAGGTCT TCAAGGCAGC CTGTGATGTC
    TTCTGTGTGG GAGATGATGT CAACATTGAA AAGGCCAACA ATTCCCTCAT CAGCAATGAT
    CGCAGCTGGA AGAGAAACAA GTTCCGCCTC ACCTTTGTGG CCGAAGCTCC AGAACTCACA
    CAAGGTCTAT CCAATGTCCA CAAAAAGCGA GTCTCAGCTG CCCGGCTCCT TAGCCGTCAA
    AACCTCCAGA GCCCTAAATC CAGTCGGTCA ACTATCTTCG TGCGTCTCCA CACCAACGGG
    AGCCAGGAGC TGCAGTACCA GCCTGGGGAC CACCTGGGTG TCTTCCCTGG CAACCACGAG
    GACCTCGTGA ATGCCCTGAT CGAGCGGCTG GAGGACGCGC CGCCTGTCAA CCAGATGGTG
    AAAGTGGAAC TGCTGGAGGA GCGGAACACG GCTTTAGGTG TCATCAGTAA CTGGACAGAC
    GAGCTCCGCC TCCCGCCCTG CACCATCTTC CAGGCCTTCA AGTACTACCT GGACATCACC
    ACGCCACCAA CGCCTCTGCA GCTGCAGCAG TTTGCCTCCC TAGCTACCAG CGAGAAGGAG
    AAGCAGCGTC TGCTGGTCCT CAGCAAGGGT TTGCAGGAGT ACGAGGAATG GAAATGGGGC
    AAGAACCCCA CCATCGTGGA GGTGCTGGAG GAGTTCCCAT CTATCCAGAT GCCGGCCACC
    CTGCTCCTGA CCCAGCTGTC CCTGCTGCAG CCCCGCTACT ATTCCATCAG CTCCTCCCCA
    GACATGTACC CTGATGAAGT GCACCTCACT GTGGCCATCG TTTCCTACCG CACTCGAGAT
    GGAGAAGGAC CAATTCACCA CGGCGTATGC TCCTCCTGGC TCAACCGGAT ACAGGCTGAC
    GAACTGGTCC CCTGTTTCGT GAGAGGAGCA CCCAGCTTCC ACCTGCCCCG GAACCCCCAA
    GTCCCCTGCA TCCTCGTTGG ACCAGGCACC GGCATTGCCC CTTTCCGAAG CTTCTGGCAA
    CAGCGGCAAT TTGATATCCA ACACAAAGGA ATGAACCCCT GCCCCATGGT CCTGGTCTTC
    GGGTGCCGGC AATCCAAGAT AGATCATATC TACAGGGAAG AGACCCTGCA GGCCAAGAAC
    AAGGGGGTCT TCAGAGAGCT GTACACGGCT TACTCCCGGG AGCCAGACAA ACCAAAGAAG
    TACGTGCAGG ACATCCTGCA GGAGCAGCTG GCGGAGTCTG TGTACCGAGC CCTGAAGGAG
    CAAGGGGGCC ACATATACGT CTGTGGGGAC GTCACCATGG CTGCTGATGT CCTCAAAGCC
    ATCCAGCGCA TCATGACCCA GCAGGGGAAG CTCTCGGCAG AGGACGCCGG CGTATTCATC
    AGCCGGATGA GGGATGACAA CCGATACCAT GAGGATATTT TTGGAGTCAC CCTGCGAACG
    TACGAAGTGA CCAACCGCCT TAGATCTGAG TCCATTGCCT TCATTGAAGA GAGCAAAAAA
    GACACCGATG AGGTTTTCAG CTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu21540
    Clone ID Related Accession (Same CDS sequence) XM_017019345.1 , XM_011538398.2 , XM_017019346.1 , NM_001204218.1
    Accession Version XM_011538398.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4407bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product nitric oxide synthase, brain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011538398.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAGGATC ACATGTTCGG TGTTCAGCAA ATCCAGCCCA ATGTCATTTC TGTTCGTCTC 
    TTCAAGCGCA AAGTTGGGGG CCTGGGATTT CTGGTGAAGG AGCGGGTCAG TAAGCCGCCC
    GTGATCATCT CTGACCTGAT TCGTGGGGGC GCCGCAGAGC AGAGTGGCCT CATCCAGGCC
    GGAGACATCA TTCTTGCGGT CAACGGCCGG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC
    CTGGAGGTAC TCAGAGGCAT TGCCTCTGAG ACCCACGTGG TCCTCATTCT GAGGGGCCCT
    GAAGGTTTCA CCACGCACCT GGAGACCACC TTTACAGGTG ATGGGACCCC CAAGACCATC
    CGGGTGACAC AGCCCCTGGG TCCCCCCACC AAAGCCGTGG ATCTGTCCCA CCAGCCACCG
    GCCGGCAAAG AACAGCCCCT GGCAGTGGAT GGGGCCTCGG GTCCCGGGAA TGGGCCTCAG
    CATGCCTACG ATGATGGGCA GGAGGCTGGC TCACTCCCCC ATGCCAACGG CCTGGCCCCC
    AGGCCCCCAG GCCAGGACCC CGCGAAGAAA GCAACCAGAG TCAGCCTCCA AGGCAGAGGG
    GAGAACAATG AACTGCTCAA GGAGATAGAG CCTGTGCTGA GCCTTCTCAC CAGTGGGAGC
    AGAGGGGTCA AGGGAGGGGC ACCTGCCAAG GCAGAGATGA AAGATATGGG AATCCAGGTG
    GACAGAGATT TGGACGGCAA GTCACACAAA CCTCTGCCCC TCGGCGTGGA GAACGACCGA
    GTCTTCAATG ACCTATGGGG GAAGGGCAAT GTGCCTGTCG TCCTCAACAA CCCATATTCA
    GAGAAGGAGC AGCCCCCCAC CTCAGGAAAA CAGTCCCCCA CAAAGAATGG CAGCCCCTCC
    AAGTGTCCAC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG AGGTGGTTCT CACTGACACC
    CTCCACCTTA AGAGCACATT GGAAACGGGA TGCACTGAGT ACATCTGCAT GGGCTCCATC
    ATGCATCCTT CTCAGCATGC AAGGAGGCCT GAAGACGTCC GCACAAAAGG ACAGCTCTTC
    CCTCTCGCCA AAGAGTTTAT TGATCAATAC TATTCATCAA TTAAAAGATT TGGCTCCAAA
    GCCCACATGG AAAGGCTGGA AGAGGTGAAC AAAGAGATCG ACACCACTAG CACTTACCAG
    CTCAAGGACA CAGAGCTCAT CTATGGGGCC AAGCACGCCT GGCGGAATGC CTCGCGCTGT
    GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTATTCGATG CCCGTGACTG CACCACGGCC
    CACGGGATGT TCAACTACAT CTGTAACCAT GTCAAGTATG CCACCAACAA AGGGAACCTC
    AGGTCTGCCA TCACCATATT CCCCCAGAGG ACAGACGGCA AGCACGACTT CCGAGTCTGG
    AACTCCCAGC TCATCCGCTA CGCTGGCTAC AAGCAGCCTG ACGGCTCCAC CCTGGGGGAC
    CCAGCCAATG TGCAGTTCAC AGAGATATGC ATACAGCAGG GCTGGAAACC GCCTAGAGGC
    CGCTTCGATG TCCTGCCGCT CCTGCTTCAG GCCAACGGCA ATGACCCTGA GCTCTTCCAG
    ATTCCTCCAG AGCTGGTGTT GGAAGTTCCC ATCAGGCACC CCAAGTTTGA GTGGTTCAAG
    GACCTGGGGC TGAAGTGGTA CGGCCTCCCC GCCGTGTCCA ACATGCTCCT AGAGATTGGC
    GGCCTGGAGT TCAGCGCCTG TCCCTTCAGT GGCTGGTACA TGGGCACAGA GATTGGTGTC
    CGCGACTACT GTGACAACTC CCGCTACAAT ATCCTGGAGG AAGTGGCCAA GAAGATGAAC
    TTAGACATGA GGAAGACGTC CTCCCTGTGG AAGGACCAGG CGCTGGTGGA GATCAATATC
    GCGGTTCTCT ATAGCTTCCA GAGTGACAAA GTGACCATTG TTGACCATCA CTCCGCCACC
    GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGGGGCTG CCCTGCCGAC
    TGGGTGTGGA TCGTGCCCCC CATGTCCGGA AGCATCACCC CTGTGTTCCA CCAGGAGATG
    CTCAACTACC GGCTCACCCC CTCCTTCGAA TACCAGCCTG ATCCCTGGAA CACGCATGTC
    TGGAAAGGCA CCAACGGGAC CCCCACAAAG CGGCGAGCCA TTGGCTTCAA GAAGCTAGCA
    GAAGCTGTCA AGTTCTCGGC CAAGCTGATG GGGCAGGCTA TGGCCAAGAG GGTGAAAGCG
    ACCATCCTCT ATGCCACAGA GACAGGCAAA TCGCAAGCTT ATGCCAAGAC CTTGTGTGAG
    ATCTTCAAAC ACGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATTGTGCAC
    CTGGAACATG AAACTCTGGT CCTTGTGGTC ACCAGCACCT TTGGCAATGG AGATCCCCCT
    GAGAATGGGG AGAAATTCGG CTGTGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAG
    GAAGAAAGGA AGTACCCGGA ACCCTTGCGT TTCTTTCCCC GTAAAGGGCC TCCCCTCCCC
    AATGGTGACA CAGAAGTCCA CGGTCTGGCT GCAGCCCGTG ACAGCCAGCA CAGGAGCTAC
    AAGGTCCGAT TCAACAGCGT CTCCTCCTAC TCTGACTCCC AAAAATCATC AGGCGATGGG
    CCCGACCTCA GAGACAACTT TGAGAGTGCT GGACCCCTGG CCAATGTGAG GTTCTCAGTT
    TTTGGCCTCG GCTCACGAGC ATACCCTCAC TTTTGCGCCT TCGGACACGC TGTGGACACC
    CTCCTGGAAG AACTGGGAGG GGAGAGGATC CTGAAGATGA GGGAAGGGGA TGAGCTCTGT
    GGGCAGGAAG AGGCTTTCAG GACCTGGGCC AAGAAGGTCT TCAAGGCAGC CTGTGATGTC
    TTCTGTGTGG GAGATGATGT CAACATTGAA AAGGCCAACA ATTCCCTCAT CAGCAATGAT
    CGCAGCTGGA AGAGAAACAA GTTCCGCCTC ACCTTTGTGG CCGAAGCTCC AGAACTCACA
    CAAGGTCTAT CCAATGTCCA CAAAAAGCGA GTCTCAGCTG CCCGGCTCCT TAGCCGTCAA
    AACCTCCAGA GCCCTAAATC CAGTCGGTCA ACTATCTTCG TGCGTCTCCA CACCAACGGG
    AGCCAGGAGC TGCAGTACCA GCCTGGGGAC CACCTGGGTG TCTTCCCTGG CAACCACGAG
    GACCTCGTGA ATGCCCTGAT CGAGCGGCTG GAGGACGCGC CGCCTGTCAA CCAGATGGTG
    AAAGTGGAAC TGCTGGAGGA GCGGAACACG GCTTTAGGTG TCATCAGTAA CTGGACAGAC
    GAGCTCCGCC TCCCGCCCTG CACCATCTTC CAGGCCTTCA AGTACTACCT GGACATCACC
    ACGCCACCAA CGCCTCTGCA GCTGCAGCAG TTTGCCTCCC TAGCTACCAG CGAGAAGGAG
    AAGCAGCGTC TGCTGGTCCT CAGCAAGGGT TTGCAGGAGT ACGAGGAATG GAAATGGGGC
    AAGAACCCCA CCATCGTGGA GGTGCTGGAG GAGTTCCCAT CTATCCAGAT GCCGGCCACC
    CTGCTCCTGA CCCAGCTGTC CCTGCTGCAG CCCCGCTACT ATTCCATCAG CTCCTCCCCA
    GACATGTACC CTGATGAAGT GCACCTCACT GTGGCCATCG TTTCCTACCG CACTCGAGAT
    GGAGAAGGAC CAATTCACCA CGGCGTATGC TCCTCCTGGC TCAACCGGAT ACAGGCTGAC
    GAACTGGTCC CCTGTTTCGT GAGAGGAGCA CCCAGCTTCC ACCTGCCCCG GAACCCCCAA
    GTCCCCTGCA TCCTCGTTGG ACCAGGCACC GGCATTGCCC CTTTCCGAAG CTTCTGGCAA
    CAGCGGCAAT TTGATATCCA ACACAAAGGA ATGAACCCCT GCCCCATGGT CCTGGTCTTC
    GGGTGCCGGC AATCCAAGAT AGATCATATC TACAGGGAAG AGACCCTGCA GGCCAAGAAC
    AAGGGGGTCT TCAGAGAGCT GTACACGGCT TACTCCCGGG AGCCAGACAA ACCAAAGAAG
    TACGTGCAGG ACATCCTGCA GGAGCAGCTG GCGGAGTCTG TGTACCGAGC CCTGAAGGAG
    CAAGGGGGCC ACATATACGT CTGTGGGGAC GTCACCATGG CTGCTGATGT CCTCAAAGCC
    ATCCAGCGCA TCATGACCCA GCAGGGGAAG CTCTCGGCAG AGGACGCCGG CGTATTCATC
    AGCCGGATGA GGGATGACAA CCGATACCAT GAGGATATTT TTGGAGTCAC CCTGCGAACG
    TACGAAGTGA CCAACCGCCT TAGATCTGAG TCCATTGCCT TCATTGAAGA GAGCAAAAAA
    GACACCGATG AGGTTTTCAG CTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011536700.1
    CDS583..4989
    Translation

    Target ORF information:

    RefSeq Version XM_011538398.2
    Organism Homo sapiens(human)
    Definition Homo sapiens nitric oxide synthase 1 (NOS1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011538398.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAGGATC ACATGTTCGG TGTTCAGCAA ATCCAGCCCA ATGTCATTTC TGTTCGTCTC 
    TTCAAGCGCA AAGTTGGGGG CCTGGGATTT CTGGTGAAGG AGCGGGTCAG TAAGCCGCCC
    GTGATCATCT CTGACCTGAT TCGTGGGGGC GCCGCAGAGC AGAGTGGCCT CATCCAGGCC
    GGAGACATCA TTCTTGCGGT CAACGGCCGG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC
    CTGGAGGTAC TCAGAGGCAT TGCCTCTGAG ACCCACGTGG TCCTCATTCT GAGGGGCCCT
    GAAGGTTTCA CCACGCACCT GGAGACCACC TTTACAGGTG ATGGGACCCC CAAGACCATC
    CGGGTGACAC AGCCCCTGGG TCCCCCCACC AAAGCCGTGG ATCTGTCCCA CCAGCCACCG
    GCCGGCAAAG AACAGCCCCT GGCAGTGGAT GGGGCCTCGG GTCCCGGGAA TGGGCCTCAG
    CATGCCTACG ATGATGGGCA GGAGGCTGGC TCACTCCCCC ATGCCAACGG CCTGGCCCCC
    AGGCCCCCAG GCCAGGACCC CGCGAAGAAA GCAACCAGAG TCAGCCTCCA AGGCAGAGGG
    GAGAACAATG AACTGCTCAA GGAGATAGAG CCTGTGCTGA GCCTTCTCAC CAGTGGGAGC
    AGAGGGGTCA AGGGAGGGGC ACCTGCCAAG GCAGAGATGA AAGATATGGG AATCCAGGTG
    GACAGAGATT TGGACGGCAA GTCACACAAA CCTCTGCCCC TCGGCGTGGA GAACGACCGA
    GTCTTCAATG ACCTATGGGG GAAGGGCAAT GTGCCTGTCG TCCTCAACAA CCCATATTCA
    GAGAAGGAGC AGCCCCCCAC CTCAGGAAAA CAGTCCCCCA CAAAGAATGG CAGCCCCTCC
    AAGTGTCCAC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG AGGTGGTTCT CACTGACACC
    CTCCACCTTA AGAGCACATT GGAAACGGGA TGCACTGAGT ACATCTGCAT GGGCTCCATC
    ATGCATCCTT CTCAGCATGC AAGGAGGCCT GAAGACGTCC GCACAAAAGG ACAGCTCTTC
    CCTCTCGCCA AAGAGTTTAT TGATCAATAC TATTCATCAA TTAAAAGATT TGGCTCCAAA
    GCCCACATGG AAAGGCTGGA AGAGGTGAAC AAAGAGATCG ACACCACTAG CACTTACCAG
    CTCAAGGACA CAGAGCTCAT CTATGGGGCC AAGCACGCCT GGCGGAATGC CTCGCGCTGT
    GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTATTCGATG CCCGTGACTG CACCACGGCC
    CACGGGATGT TCAACTACAT CTGTAACCAT GTCAAGTATG CCACCAACAA AGGGAACCTC
    AGGTCTGCCA TCACCATATT CCCCCAGAGG ACAGACGGCA AGCACGACTT CCGAGTCTGG
    AACTCCCAGC TCATCCGCTA CGCTGGCTAC AAGCAGCCTG ACGGCTCCAC CCTGGGGGAC
    CCAGCCAATG TGCAGTTCAC AGAGATATGC ATACAGCAGG GCTGGAAACC GCCTAGAGGC
    CGCTTCGATG TCCTGCCGCT CCTGCTTCAG GCCAACGGCA ATGACCCTGA GCTCTTCCAG
    ATTCCTCCAG AGCTGGTGTT GGAAGTTCCC ATCAGGCACC CCAAGTTTGA GTGGTTCAAG
    GACCTGGGGC TGAAGTGGTA CGGCCTCCCC GCCGTGTCCA ACATGCTCCT AGAGATTGGC
    GGCCTGGAGT TCAGCGCCTG TCCCTTCAGT GGCTGGTACA TGGGCACAGA GATTGGTGTC
    CGCGACTACT GTGACAACTC CCGCTACAAT ATCCTGGAGG AAGTGGCCAA GAAGATGAAC
    TTAGACATGA GGAAGACGTC CTCCCTGTGG AAGGACCAGG CGCTGGTGGA GATCAATATC
    GCGGTTCTCT ATAGCTTCCA GAGTGACAAA GTGACCATTG TTGACCATCA CTCCGCCACC
    GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGGGGCTG CCCTGCCGAC
    TGGGTGTGGA TCGTGCCCCC CATGTCCGGA AGCATCACCC CTGTGTTCCA CCAGGAGATG
    CTCAACTACC GGCTCACCCC CTCCTTCGAA TACCAGCCTG ATCCCTGGAA CACGCATGTC
    TGGAAAGGCA CCAACGGGAC CCCCACAAAG CGGCGAGCCA TTGGCTTCAA GAAGCTAGCA
    GAAGCTGTCA AGTTCTCGGC CAAGCTGATG GGGCAGGCTA TGGCCAAGAG GGTGAAAGCG
    ACCATCCTCT ATGCCACAGA GACAGGCAAA TCGCAAGCTT ATGCCAAGAC CTTGTGTGAG
    ATCTTCAAAC ACGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATTGTGCAC
    CTGGAACATG AAACTCTGGT CCTTGTGGTC ACCAGCACCT TTGGCAATGG AGATCCCCCT
    GAGAATGGGG AGAAATTCGG CTGTGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAG
    GAAGAAAGGA AGTACCCGGA ACCCTTGCGT TTCTTTCCCC GTAAAGGGCC TCCCCTCCCC
    AATGGTGACA CAGAAGTCCA CGGTCTGGCT GCAGCCCGTG ACAGCCAGCA CAGGAGCTAC
    AAGGTCCGAT TCAACAGCGT CTCCTCCTAC TCTGACTCCC AAAAATCATC AGGCGATGGG
    CCCGACCTCA GAGACAACTT TGAGAGTGCT GGACCCCTGG CCAATGTGAG GTTCTCAGTT
    TTTGGCCTCG GCTCACGAGC ATACCCTCAC TTTTGCGCCT TCGGACACGC TGTGGACACC
    CTCCTGGAAG AACTGGGAGG GGAGAGGATC CTGAAGATGA GGGAAGGGGA TGAGCTCTGT
    GGGCAGGAAG AGGCTTTCAG GACCTGGGCC AAGAAGGTCT TCAAGGCAGC CTGTGATGTC
    TTCTGTGTGG GAGATGATGT CAACATTGAA AAGGCCAACA ATTCCCTCAT CAGCAATGAT
    CGCAGCTGGA AGAGAAACAA GTTCCGCCTC ACCTTTGTGG CCGAAGCTCC AGAACTCACA
    CAAGGTCTAT CCAATGTCCA CAAAAAGCGA GTCTCAGCTG CCCGGCTCCT TAGCCGTCAA
    AACCTCCAGA GCCCTAAATC CAGTCGGTCA ACTATCTTCG TGCGTCTCCA CACCAACGGG
    AGCCAGGAGC TGCAGTACCA GCCTGGGGAC CACCTGGGTG TCTTCCCTGG CAACCACGAG
    GACCTCGTGA ATGCCCTGAT CGAGCGGCTG GAGGACGCGC CGCCTGTCAA CCAGATGGTG
    AAAGTGGAAC TGCTGGAGGA GCGGAACACG GCTTTAGGTG TCATCAGTAA CTGGACAGAC
    GAGCTCCGCC TCCCGCCCTG CACCATCTTC CAGGCCTTCA AGTACTACCT GGACATCACC
    ACGCCACCAA CGCCTCTGCA GCTGCAGCAG TTTGCCTCCC TAGCTACCAG CGAGAAGGAG
    AAGCAGCGTC TGCTGGTCCT CAGCAAGGGT TTGCAGGAGT ACGAGGAATG GAAATGGGGC
    AAGAACCCCA CCATCGTGGA GGTGCTGGAG GAGTTCCCAT CTATCCAGAT GCCGGCCACC
    CTGCTCCTGA CCCAGCTGTC CCTGCTGCAG CCCCGCTACT ATTCCATCAG CTCCTCCCCA
    GACATGTACC CTGATGAAGT GCACCTCACT GTGGCCATCG TTTCCTACCG CACTCGAGAT
    GGAGAAGGAC CAATTCACCA CGGCGTATGC TCCTCCTGGC TCAACCGGAT ACAGGCTGAC
    GAACTGGTCC CCTGTTTCGT GAGAGGAGCA CCCAGCTTCC ACCTGCCCCG GAACCCCCAA
    GTCCCCTGCA TCCTCGTTGG ACCAGGCACC GGCATTGCCC CTTTCCGAAG CTTCTGGCAA
    CAGCGGCAAT TTGATATCCA ACACAAAGGA ATGAACCCCT GCCCCATGGT CCTGGTCTTC
    GGGTGCCGGC AATCCAAGAT AGATCATATC TACAGGGAAG AGACCCTGCA GGCCAAGAAC
    AAGGGGGTCT TCAGAGAGCT GTACACGGCT TACTCCCGGG AGCCAGACAA ACCAAAGAAG
    TACGTGCAGG ACATCCTGCA GGAGCAGCTG GCGGAGTCTG TGTACCGAGC CCTGAAGGAG
    CAAGGGGGCC ACATATACGT CTGTGGGGAC GTCACCATGG CTGCTGATGT CCTCAAAGCC
    ATCCAGCGCA TCATGACCCA GCAGGGGAAG CTCTCGGCAG AGGACGCCGG CGTATTCATC
    AGCCGGATGA GGGATGACAA CCGATACCAT GAGGATATTT TTGGAGTCAC CCTGCGAACG
    TACGAAGTGA CCAACCGCCT TAGATCTGAG TCCATTGCCT TCATTGAAGA GAGCAAAAAA
    GACACCGATG AGGTTTTCAG CTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu21540
    Clone ID Related Accession (Same CDS sequence) XM_017019345.1 , XM_011538398.2 , XM_017019346.1 , NM_001204218.1
    Accession Version XM_017019346.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4407bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product nitric oxide synthase, brain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAGGATC ACATGTTCGG TGTTCAGCAA ATCCAGCCCA ATGTCATTTC TGTTCGTCTC 
    TTCAAGCGCA AAGTTGGGGG CCTGGGATTT CTGGTGAAGG AGCGGGTCAG TAAGCCGCCC
    GTGATCATCT CTGACCTGAT TCGTGGGGGC GCCGCAGAGC AGAGTGGCCT CATCCAGGCC
    GGAGACATCA TTCTTGCGGT CAACGGCCGG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC
    CTGGAGGTAC TCAGAGGCAT TGCCTCTGAG ACCCACGTGG TCCTCATTCT GAGGGGCCCT
    GAAGGTTTCA CCACGCACCT GGAGACCACC TTTACAGGTG ATGGGACCCC CAAGACCATC
    CGGGTGACAC AGCCCCTGGG TCCCCCCACC AAAGCCGTGG ATCTGTCCCA CCAGCCACCG
    GCCGGCAAAG AACAGCCCCT GGCAGTGGAT GGGGCCTCGG GTCCCGGGAA TGGGCCTCAG
    CATGCCTACG ATGATGGGCA GGAGGCTGGC TCACTCCCCC ATGCCAACGG CCTGGCCCCC
    AGGCCCCCAG GCCAGGACCC CGCGAAGAAA GCAACCAGAG TCAGCCTCCA AGGCAGAGGG
    GAGAACAATG AACTGCTCAA GGAGATAGAG CCTGTGCTGA GCCTTCTCAC CAGTGGGAGC
    AGAGGGGTCA AGGGAGGGGC ACCTGCCAAG GCAGAGATGA AAGATATGGG AATCCAGGTG
    GACAGAGATT TGGACGGCAA GTCACACAAA CCTCTGCCCC TCGGCGTGGA GAACGACCGA
    GTCTTCAATG ACCTATGGGG GAAGGGCAAT GTGCCTGTCG TCCTCAACAA CCCATATTCA
    GAGAAGGAGC AGCCCCCCAC CTCAGGAAAA CAGTCCCCCA CAAAGAATGG CAGCCCCTCC
    AAGTGTCCAC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG AGGTGGTTCT CACTGACACC
    CTCCACCTTA AGAGCACATT GGAAACGGGA TGCACTGAGT ACATCTGCAT GGGCTCCATC
    ATGCATCCTT CTCAGCATGC AAGGAGGCCT GAAGACGTCC GCACAAAAGG ACAGCTCTTC
    CCTCTCGCCA AAGAGTTTAT TGATCAATAC TATTCATCAA TTAAAAGATT TGGCTCCAAA
    GCCCACATGG AAAGGCTGGA AGAGGTGAAC AAAGAGATCG ACACCACTAG CACTTACCAG
    CTCAAGGACA CAGAGCTCAT CTATGGGGCC AAGCACGCCT GGCGGAATGC CTCGCGCTGT
    GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTATTCGATG CCCGTGACTG CACCACGGCC
    CACGGGATGT TCAACTACAT CTGTAACCAT GTCAAGTATG CCACCAACAA AGGGAACCTC
    AGGTCTGCCA TCACCATATT CCCCCAGAGG ACAGACGGCA AGCACGACTT CCGAGTCTGG
    AACTCCCAGC TCATCCGCTA CGCTGGCTAC AAGCAGCCTG ACGGCTCCAC CCTGGGGGAC
    CCAGCCAATG TGCAGTTCAC AGAGATATGC ATACAGCAGG GCTGGAAACC GCCTAGAGGC
    CGCTTCGATG TCCTGCCGCT CCTGCTTCAG GCCAACGGCA ATGACCCTGA GCTCTTCCAG
    ATTCCTCCAG AGCTGGTGTT GGAAGTTCCC ATCAGGCACC CCAAGTTTGA GTGGTTCAAG
    GACCTGGGGC TGAAGTGGTA CGGCCTCCCC GCCGTGTCCA ACATGCTCCT AGAGATTGGC
    GGCCTGGAGT TCAGCGCCTG TCCCTTCAGT GGCTGGTACA TGGGCACAGA GATTGGTGTC
    CGCGACTACT GTGACAACTC CCGCTACAAT ATCCTGGAGG AAGTGGCCAA GAAGATGAAC
    TTAGACATGA GGAAGACGTC CTCCCTGTGG AAGGACCAGG CGCTGGTGGA GATCAATATC
    GCGGTTCTCT ATAGCTTCCA GAGTGACAAA GTGACCATTG TTGACCATCA CTCCGCCACC
    GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGGGGCTG CCCTGCCGAC
    TGGGTGTGGA TCGTGCCCCC CATGTCCGGA AGCATCACCC CTGTGTTCCA CCAGGAGATG
    CTCAACTACC GGCTCACCCC CTCCTTCGAA TACCAGCCTG ATCCCTGGAA CACGCATGTC
    TGGAAAGGCA CCAACGGGAC CCCCACAAAG CGGCGAGCCA TTGGCTTCAA GAAGCTAGCA
    GAAGCTGTCA AGTTCTCGGC CAAGCTGATG GGGCAGGCTA TGGCCAAGAG GGTGAAAGCG
    ACCATCCTCT ATGCCACAGA GACAGGCAAA TCGCAAGCTT ATGCCAAGAC CTTGTGTGAG
    ATCTTCAAAC ACGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATTGTGCAC
    CTGGAACATG AAACTCTGGT CCTTGTGGTC ACCAGCACCT TTGGCAATGG AGATCCCCCT
    GAGAATGGGG AGAAATTCGG CTGTGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAG
    GAAGAAAGGA AGTACCCGGA ACCCTTGCGT TTCTTTCCCC GTAAAGGGCC TCCCCTCCCC
    AATGGTGACA CAGAAGTCCA CGGTCTGGCT GCAGCCCGTG ACAGCCAGCA CAGGAGCTAC
    AAGGTCCGAT TCAACAGCGT CTCCTCCTAC TCTGACTCCC AAAAATCATC AGGCGATGGG
    CCCGACCTCA GAGACAACTT TGAGAGTGCT GGACCCCTGG CCAATGTGAG GTTCTCAGTT
    TTTGGCCTCG GCTCACGAGC ATACCCTCAC TTTTGCGCCT TCGGACACGC TGTGGACACC
    CTCCTGGAAG AACTGGGAGG GGAGAGGATC CTGAAGATGA GGGAAGGGGA TGAGCTCTGT
    GGGCAGGAAG AGGCTTTCAG GACCTGGGCC AAGAAGGTCT TCAAGGCAGC CTGTGATGTC
    TTCTGTGTGG GAGATGATGT CAACATTGAA AAGGCCAACA ATTCCCTCAT CAGCAATGAT
    CGCAGCTGGA AGAGAAACAA GTTCCGCCTC ACCTTTGTGG CCGAAGCTCC AGAACTCACA
    CAAGGTCTAT CCAATGTCCA CAAAAAGCGA GTCTCAGCTG CCCGGCTCCT TAGCCGTCAA
    AACCTCCAGA GCCCTAAATC CAGTCGGTCA ACTATCTTCG TGCGTCTCCA CACCAACGGG
    AGCCAGGAGC TGCAGTACCA GCCTGGGGAC CACCTGGGTG TCTTCCCTGG CAACCACGAG
    GACCTCGTGA ATGCCCTGAT CGAGCGGCTG GAGGACGCGC CGCCTGTCAA CCAGATGGTG
    AAAGTGGAAC TGCTGGAGGA GCGGAACACG GCTTTAGGTG TCATCAGTAA CTGGACAGAC
    GAGCTCCGCC TCCCGCCCTG CACCATCTTC CAGGCCTTCA AGTACTACCT GGACATCACC
    ACGCCACCAA CGCCTCTGCA GCTGCAGCAG TTTGCCTCCC TAGCTACCAG CGAGAAGGAG
    AAGCAGCGTC TGCTGGTCCT CAGCAAGGGT TTGCAGGAGT ACGAGGAATG GAAATGGGGC
    AAGAACCCCA CCATCGTGGA GGTGCTGGAG GAGTTCCCAT CTATCCAGAT GCCGGCCACC
    CTGCTCCTGA CCCAGCTGTC CCTGCTGCAG CCCCGCTACT ATTCCATCAG CTCCTCCCCA
    GACATGTACC CTGATGAAGT GCACCTCACT GTGGCCATCG TTTCCTACCG CACTCGAGAT
    GGAGAAGGAC CAATTCACCA CGGCGTATGC TCCTCCTGGC TCAACCGGAT ACAGGCTGAC
    GAACTGGTCC CCTGTTTCGT GAGAGGAGCA CCCAGCTTCC ACCTGCCCCG GAACCCCCAA
    GTCCCCTGCA TCCTCGTTGG ACCAGGCACC GGCATTGCCC CTTTCCGAAG CTTCTGGCAA
    CAGCGGCAAT TTGATATCCA ACACAAAGGA ATGAACCCCT GCCCCATGGT CCTGGTCTTC
    GGGTGCCGGC AATCCAAGAT AGATCATATC TACAGGGAAG AGACCCTGCA GGCCAAGAAC
    AAGGGGGTCT TCAGAGAGCT GTACACGGCT TACTCCCGGG AGCCAGACAA ACCAAAGAAG
    TACGTGCAGG ACATCCTGCA GGAGCAGCTG GCGGAGTCTG TGTACCGAGC CCTGAAGGAG
    CAAGGGGGCC ACATATACGT CTGTGGGGAC GTCACCATGG CTGCTGATGT CCTCAAAGCC
    ATCCAGCGCA TCATGACCCA GCAGGGGAAG CTCTCGGCAG AGGACGCCGG CGTATTCATC
    AGCCGGATGA GGGATGACAA CCGATACCAT GAGGATATTT TTGGAGTCAC CCTGCGAACG
    TACGAAGTGA CCAACCGCCT TAGATCTGAG TCCATTGCCT TCATTGAAGA GAGCAAAAAA
    GACACCGATG AGGTTTTCAG CTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016874835.1
    CDS533..4939
    Translation

    Target ORF information:

    RefSeq Version XM_017019346.1
    Organism Homo sapiens(human)
    Definition Homo sapiens nitric oxide synthase 1 (NOS1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017019346.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAGGATC ACATGTTCGG TGTTCAGCAA ATCCAGCCCA ATGTCATTTC TGTTCGTCTC 
    TTCAAGCGCA AAGTTGGGGG CCTGGGATTT CTGGTGAAGG AGCGGGTCAG TAAGCCGCCC
    GTGATCATCT CTGACCTGAT TCGTGGGGGC GCCGCAGAGC AGAGTGGCCT CATCCAGGCC
    GGAGACATCA TTCTTGCGGT CAACGGCCGG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC
    CTGGAGGTAC TCAGAGGCAT TGCCTCTGAG ACCCACGTGG TCCTCATTCT GAGGGGCCCT
    GAAGGTTTCA CCACGCACCT GGAGACCACC TTTACAGGTG ATGGGACCCC CAAGACCATC
    CGGGTGACAC AGCCCCTGGG TCCCCCCACC AAAGCCGTGG ATCTGTCCCA CCAGCCACCG
    GCCGGCAAAG AACAGCCCCT GGCAGTGGAT GGGGCCTCGG GTCCCGGGAA TGGGCCTCAG
    CATGCCTACG ATGATGGGCA GGAGGCTGGC TCACTCCCCC ATGCCAACGG CCTGGCCCCC
    AGGCCCCCAG GCCAGGACCC CGCGAAGAAA GCAACCAGAG TCAGCCTCCA AGGCAGAGGG
    GAGAACAATG AACTGCTCAA GGAGATAGAG CCTGTGCTGA GCCTTCTCAC CAGTGGGAGC
    AGAGGGGTCA AGGGAGGGGC ACCTGCCAAG GCAGAGATGA AAGATATGGG AATCCAGGTG
    GACAGAGATT TGGACGGCAA GTCACACAAA CCTCTGCCCC TCGGCGTGGA GAACGACCGA
    GTCTTCAATG ACCTATGGGG GAAGGGCAAT GTGCCTGTCG TCCTCAACAA CCCATATTCA
    GAGAAGGAGC AGCCCCCCAC CTCAGGAAAA CAGTCCCCCA CAAAGAATGG CAGCCCCTCC
    AAGTGTCCAC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG AGGTGGTTCT CACTGACACC
    CTCCACCTTA AGAGCACATT GGAAACGGGA TGCACTGAGT ACATCTGCAT GGGCTCCATC
    ATGCATCCTT CTCAGCATGC AAGGAGGCCT GAAGACGTCC GCACAAAAGG ACAGCTCTTC
    CCTCTCGCCA AAGAGTTTAT TGATCAATAC TATTCATCAA TTAAAAGATT TGGCTCCAAA
    GCCCACATGG AAAGGCTGGA AGAGGTGAAC AAAGAGATCG ACACCACTAG CACTTACCAG
    CTCAAGGACA CAGAGCTCAT CTATGGGGCC AAGCACGCCT GGCGGAATGC CTCGCGCTGT
    GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTATTCGATG CCCGTGACTG CACCACGGCC
    CACGGGATGT TCAACTACAT CTGTAACCAT GTCAAGTATG CCACCAACAA AGGGAACCTC
    AGGTCTGCCA TCACCATATT CCCCCAGAGG ACAGACGGCA AGCACGACTT CCGAGTCTGG
    AACTCCCAGC TCATCCGCTA CGCTGGCTAC AAGCAGCCTG ACGGCTCCAC CCTGGGGGAC
    CCAGCCAATG TGCAGTTCAC AGAGATATGC ATACAGCAGG GCTGGAAACC GCCTAGAGGC
    CGCTTCGATG TCCTGCCGCT CCTGCTTCAG GCCAACGGCA ATGACCCTGA GCTCTTCCAG
    ATTCCTCCAG AGCTGGTGTT GGAAGTTCCC ATCAGGCACC CCAAGTTTGA GTGGTTCAAG
    GACCTGGGGC TGAAGTGGTA CGGCCTCCCC GCCGTGTCCA ACATGCTCCT AGAGATTGGC
    GGCCTGGAGT TCAGCGCCTG TCCCTTCAGT GGCTGGTACA TGGGCACAGA GATTGGTGTC
    CGCGACTACT GTGACAACTC CCGCTACAAT ATCCTGGAGG AAGTGGCCAA GAAGATGAAC
    TTAGACATGA GGAAGACGTC CTCCCTGTGG AAGGACCAGG CGCTGGTGGA GATCAATATC
    GCGGTTCTCT ATAGCTTCCA GAGTGACAAA GTGACCATTG TTGACCATCA CTCCGCCACC
    GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGGGGCTG CCCTGCCGAC
    TGGGTGTGGA TCGTGCCCCC CATGTCCGGA AGCATCACCC CTGTGTTCCA CCAGGAGATG
    CTCAACTACC GGCTCACCCC CTCCTTCGAA TACCAGCCTG ATCCCTGGAA CACGCATGTC
    TGGAAAGGCA CCAACGGGAC CCCCACAAAG CGGCGAGCCA TTGGCTTCAA GAAGCTAGCA
    GAAGCTGTCA AGTTCTCGGC CAAGCTGATG GGGCAGGCTA TGGCCAAGAG GGTGAAAGCG
    ACCATCCTCT ATGCCACAGA GACAGGCAAA TCGCAAGCTT ATGCCAAGAC CTTGTGTGAG
    ATCTTCAAAC ACGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATTGTGCAC
    CTGGAACATG AAACTCTGGT CCTTGTGGTC ACCAGCACCT TTGGCAATGG AGATCCCCCT
    GAGAATGGGG AGAAATTCGG CTGTGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAG
    GAAGAAAGGA AGTACCCGGA ACCCTTGCGT TTCTTTCCCC GTAAAGGGCC TCCCCTCCCC
    AATGGTGACA CAGAAGTCCA CGGTCTGGCT GCAGCCCGTG ACAGCCAGCA CAGGAGCTAC
    AAGGTCCGAT TCAACAGCGT CTCCTCCTAC TCTGACTCCC AAAAATCATC AGGCGATGGG
    CCCGACCTCA GAGACAACTT TGAGAGTGCT GGACCCCTGG CCAATGTGAG GTTCTCAGTT
    TTTGGCCTCG GCTCACGAGC ATACCCTCAC TTTTGCGCCT TCGGACACGC TGTGGACACC
    CTCCTGGAAG AACTGGGAGG GGAGAGGATC CTGAAGATGA GGGAAGGGGA TGAGCTCTGT
    GGGCAGGAAG AGGCTTTCAG GACCTGGGCC AAGAAGGTCT TCAAGGCAGC CTGTGATGTC
    TTCTGTGTGG GAGATGATGT CAACATTGAA AAGGCCAACA ATTCCCTCAT CAGCAATGAT
    CGCAGCTGGA AGAGAAACAA GTTCCGCCTC ACCTTTGTGG CCGAAGCTCC AGAACTCACA
    CAAGGTCTAT CCAATGTCCA CAAAAAGCGA GTCTCAGCTG CCCGGCTCCT TAGCCGTCAA
    AACCTCCAGA GCCCTAAATC CAGTCGGTCA ACTATCTTCG TGCGTCTCCA CACCAACGGG
    AGCCAGGAGC TGCAGTACCA GCCTGGGGAC CACCTGGGTG TCTTCCCTGG CAACCACGAG
    GACCTCGTGA ATGCCCTGAT CGAGCGGCTG GAGGACGCGC CGCCTGTCAA CCAGATGGTG
    AAAGTGGAAC TGCTGGAGGA GCGGAACACG GCTTTAGGTG TCATCAGTAA CTGGACAGAC
    GAGCTCCGCC TCCCGCCCTG CACCATCTTC CAGGCCTTCA AGTACTACCT GGACATCACC
    ACGCCACCAA CGCCTCTGCA GCTGCAGCAG TTTGCCTCCC TAGCTACCAG CGAGAAGGAG
    AAGCAGCGTC TGCTGGTCCT CAGCAAGGGT TTGCAGGAGT ACGAGGAATG GAAATGGGGC
    AAGAACCCCA CCATCGTGGA GGTGCTGGAG GAGTTCCCAT CTATCCAGAT GCCGGCCACC
    CTGCTCCTGA CCCAGCTGTC CCTGCTGCAG CCCCGCTACT ATTCCATCAG CTCCTCCCCA
    GACATGTACC CTGATGAAGT GCACCTCACT GTGGCCATCG TTTCCTACCG CACTCGAGAT
    GGAGAAGGAC CAATTCACCA CGGCGTATGC TCCTCCTGGC TCAACCGGAT ACAGGCTGAC
    GAACTGGTCC CCTGTTTCGT GAGAGGAGCA CCCAGCTTCC ACCTGCCCCG GAACCCCCAA
    GTCCCCTGCA TCCTCGTTGG ACCAGGCACC GGCATTGCCC CTTTCCGAAG CTTCTGGCAA
    CAGCGGCAAT TTGATATCCA ACACAAAGGA ATGAACCCCT GCCCCATGGT CCTGGTCTTC
    GGGTGCCGGC AATCCAAGAT AGATCATATC TACAGGGAAG AGACCCTGCA GGCCAAGAAC
    AAGGGGGTCT TCAGAGAGCT GTACACGGCT TACTCCCGGG AGCCAGACAA ACCAAAGAAG
    TACGTGCAGG ACATCCTGCA GGAGCAGCTG GCGGAGTCTG TGTACCGAGC CCTGAAGGAG
    CAAGGGGGCC ACATATACGT CTGTGGGGAC GTCACCATGG CTGCTGATGT CCTCAAAGCC
    ATCCAGCGCA TCATGACCCA GCAGGGGAAG CTCTCGGCAG AGGACGCCGG CGTATTCATC
    AGCCGGATGA GGGATGACAA CCGATACCAT GAGGATATTT TTGGAGTCAC CCTGCGAACG
    TACGAAGTGA CCAACCGCCT TAGATCTGAG TCCATTGCCT TCATTGAAGA GAGCAAAAAA
    GACACCGATG AGGTTTTCAG CTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.