NOS1 cDNA ORF clone, Pan troglodytes(chimpanzee)

The following NOS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NOS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OPc66615 XM_016924328.2
Latest version!
Pan troglodytes nitric oxide synthase 1 (NOS1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OPc66615 XM_016924329.2
Latest version!
Pan troglodytes nitric oxide synthase 1 (NOS1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OPc66615 XM_016924331.2
Latest version!
Pan troglodytes nitric oxide synthase 1 (NOS1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OPc66615 XM_016924332.2
Latest version!
Pan troglodytes nitric oxide synthase 1 (NOS1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OPc66615 XM_016924327.2
Latest version!
Pan troglodytes nitric oxide synthase 1 (NOS1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OPc66616 XM_009426346.3
Latest version!
Pan troglodytes nitric oxide synthase 1 (NOS1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
View Old Accession Versions >>
OPc66615 XM_016924331.1 Pan troglodytes nitric oxide synthase 1 (NOS1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OPc66615 XM_016924327.1 Pan troglodytes nitric oxide synthase 1 (NOS1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OPc66615 XM_016924332.1 Pan troglodytes nitric oxide synthase 1 (NOS1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OPc66615 XM_016924328.1 Pan troglodytes nitric oxide synthase 1 (NOS1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OPc66615 XM_016924329.1 Pan troglodytes nitric oxide synthase 1 (NOS1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OPc66616 XM_009426346.2 Pan troglodytes nitric oxide synthase 1 (NOS1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OPc66615
    Clone ID Related Accession (Same CDS sequence) XM_016924331.1 , XM_016924327.2 , XM_016924331.2 , XM_016924328.1 , XM_016924332.2 , XM_016924327.1 , XM_016924332.1 , XM_016924328.2 , XM_016924329.2 , XM_016924329.1
    Accession Version XM_016924331.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4407bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-01
    Organism Pan troglodytes(chimpanzee)
    Product nitric oxide synthase, brain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006479.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAGGATC ACATGTTCGG TGTTCAGCAA ATCCAGCCCA ATGTCATTTC TGTTCGTCTC 
    TTCAAGCGCA AAGTTGGGGG CCTGGGATTC CTGGTGAAGG AGCGGGTCAG TAAGCCGCCC
    GTGATTATCT CTGACCTGAT TCGTGGGGGC GCCGCAGAGC AGAGTGGCCT CATCCAGGCC
    GGAGACATCA TTCTTGCGGT CAACGGCCGG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC
    CTGGAGGTAC TCAGAGGCAT TGCCTCCGAG ACCCACGTGG TCCTCATTCT GAGGGGTCCT
    GAAGGTTTCA CCACGCACCT GGAGACCACC TTTACAGGTG ATGGGACCCC CAAGACCATC
    CGGGTGACAC AGCCCCTGGG TCCCCCCACC AAAGCCGTGG ATCTGTCCCA CCAGCCATCG
    GCCGGCAAAG AACAGCCGCT GGCAGTGGAT GGGGCCTCGG GTCCCGGGAA TGGGCCTCAG
    CATGCCTACG ATGACGGGCA GGAGGCTGGC TCACTCCCCC ATGCCAACGG CCTGGCCCCC
    AGGCCCCCAG GCCAGGACCC TGCGAAGAAA GCAACCAGAG TCAGCCTCCA AGGCAGAGGG
    GAGAACAATG AACTGCTCAA GGAGATAGAG CCTGTGCTGA GCCTTCTCAC CAGTGGGAGC
    AGAGGGGTCA AGGGAGGGGC ACCTGCCAAG GCAGAGATGA AAGATATGGG AATCCAGGTG
    GACAGAGATT TGGACGGCAA GTCACACAAA CCTCTGCCCC TCGCCGTGGA GAATGACCGA
    GTCTTCAATG ACCTATGGGG GAAGGGCAAT GTGCCTGTCG TCCTCAACAA CCCATATTCA
    GAGAAGGAGC AGCCCCCTGC CTCAGGAAAA CAGTCCCCCA CAAAGAATGG CAGCCCCTCC
    AAGTGTCCAC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG AGGTGGTTCT CACTGACACC
    CTCCACCTTA AGAGCACATT GGAAACGGGA TGCACTGAGT ACATCTGCAT GGGCTCCATC
    ATGCATCCTT CTCAGCATGC AAGGAGGCCT GAAGATGTCC GCACAAAAGA ACAGCTCTTC
    CCTCTCGCCA AAGAGTTTAT TGATCAATAC TATTCATCAA TTAAAAGATT TGGCTCCAAA
    GCCCACATGG AAAGGCTGGA AGAGGTGAAC AAAGAGATCG AAACCACTAG CACCTACCAG
    CTCAAGGACA CAGAGCTCAT CTATGGGGCC AAGCACGCCT GGCGGAATGC CTCGCGCTGT
    GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTATTCGATG CCCGTGACTG CACCACGGCC
    CACGGGATGT TCAACTACAT CTGTAACCAC GTCAAGTATG CCACCAACAA AGGGAACCTC
    AGGTCCGCCA TCACCATATT CCCCCAGAGG ACAGACGGCA AGCACGACTT CCGAGTCTGG
    AACTCCCAGC TCATCCGCTA CGCTGGCTAC AAGCAGCCCG ACGGCTCCAC CCTGGGGGAC
    CCAGCCAATG TGCAGTTCAC AGAGATATGC ATACAGCAGG GCTGGAAACC GCCTAGAGGC
    CGCTTCGATG TCCTGCCGCT CCTGCTTCAG GCCAACGGCA ATGACCCTGA GCTCTTCCAG
    ATTCCTCCAG AGCTGGTGTT GGAAGTTCCC ATCAGGCACC CCAAGTTTGA GTGGTTCAAG
    GACCTGGGGC TGAAGTGGTA CGGCCTCCCC GCCGTGTCCA ACATGCTCCT AGAGATTGGC
    GGCCTGGAGT TCAGCGCCTG TCCCTTCAGT GGCTGGTACA TGGGCACAGA GATTGGTGTC
    CGCGACTACT GTGACAACTC CCGCTACAAT ATCCTGGAGG AAGTGGCCAA GAAGATGAAC
    TTAGACATGA GGAAGATGTC CTCCCTGTGG AAGGACCAGG CGCTGGTGGA GATCAATATC
    GCGGTTCTCT ACAGCTTCCA GAGTGACAAA GTGACCATTG TTGACCATCA CTCCGCCACC
    GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGGGGCTG CCCTGCCGAC
    TGGGTGTGGA TCGTGCCCCC CATGTCCGGA AGCATCACCC CTGTGTTCCA CCAGGAGATG
    CTCAACTACC GGCTCACCCC CTCCTTCGAA TACCAGCCTG ATCCCTGGAA CACGCATGTC
    TGGAAAGGCA CCAACGGGAC CCCCACAAAG CGGCGAGCCA TCGGCTTCAA GAAGCTAGCA
    GAAGCTGTCA AGTTCTCGGC CAAGCTGATG GGGCAGGCTA TGGCCAAGAG GGTCAAAGCG
    ACCATCCTCT ATGCCACAGA GACAGGCAAA TCGCAAGCTT ATGCCAAGAC CTTGTGTGAG
    ATCTTCAAAC ACGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATCGTGCAC
    CTGGAACATG AAACTCTGGT CCTCGTGGTC ACCAGCACCT TTGGCAATGG AGATCCCCCT
    GAGAATGGGG AGAAATTCGG CTGTGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAG
    GAAGAAAGGA AGTACCCGGA ACCCTTGCGT TTCTTTCCCC GTAAAGGGCC TCCCCTCCCC
    AATGGTGACA CAGAAGTCCA CGGTCTGGCT GCAGCCCGTG ACAGCCAGCA CAGGAGCTAC
    AAGGTCCGAT TCAACAGCGT CTCCTCCTAC TCTGACTCCC AAAAATCATC AGGCGATGGG
    CCCGACCTCA GAGACAACTT TGAGAGTGCT GGACCCCTGG CCAATGTGAG GTTCTCAGTT
    TTTGGCCTCG GCTCACGAGC ATACCCTCAC TTTTGTGCCT TCGGACACGC TGTGGACACC
    CTCCTGGAAG AACTGGGAGG GGAGAGGATC CTGAAGATGA GGGAAGGGGA TGAGCTCTGT
    GGGCAGGAAG AGGCTTTCAG GACCTGGGCC AAGAAGGTCT TCAAGGCAGC CTGTGATGTC
    TTCTGTGTGG GAGATGATGT CAACATTGAA AAGGCCAACA ATTCCCTCAT CAGCAATGAT
    CGCAGCTGGA AGAGAAACAA GTTCCGCCTC ACCTTTGTGG CCGAAGCTCC AGAACTCACA
    CAAGGTCTAT CCAATGTCCA CAAAAAGCGA GTCTCAGCTG CCCGGCTCCT TAGCCGTCAA
    AACCTCCAGA GTCCTAAATC CAGTCGGTCA ACTATCTTCG TGCGTCTCCA CACCAACGGG
    AACCAGGAGC TGCAGTACCA GCCTGGGGAC CACCTGGGTG TCTTCCCTGG CAACCACGAG
    GACCTCGTGA ATGCCCTGAT CGAGCGGCTG GAGGACGCGC CCCCTGTCAA CCAGATGGTG
    AAAGTGGAAC TGCTGGAGGA GCGGAACACG GCTTTAGGTG TCATCAGTAA CTGGACAGAC
    GAGCTCCGCC TCCCGCCCTG CACCATCTTC CAGGCCTTCA AGTACTACCT GGACATCACC
    ACGCCACCAA CGCCCCTGCA GCTGCAGCAG TTTGCCTCCC TAGCTACCAG CGAGAAGGAG
    AAGCAGCGTC TGCTGGTCCT CAGCAAGGGT TTGCAGGAGT ACGAGGAATG GAAATGGGGC
    AAGAACCCCA CCATCGTGGA GGTGCTGGAG GAATTCCCAT CTATCCAGAT GCCGGCCACC
    CTGCTCCTGA CCCAGCTGTC CCTGCTGCAG CCCCGCTACT ATTCCATCAG CTCCTCCCCA
    GACATGTACC CTGATGAAGT GCACCTCACT GTGGCCATCG TTTCCTACCG CACTCGAGAT
    GGAGAAGGAC CAATTCACCA CGGCGTATGC TCCTCCTGGC TCAACCGGAT ACAGGCTGAC
    GAACTGGTCC CCTGTTTCGT GAGAGGAGCA CCCAGCTTCC ACCTGCCCCG GAACCCCCAA
    GTCCCCTGCA TCCTCGTTGG ACCAGGCACC GGCATTGCCC CTTTCCGAAG CTTCTGGCAA
    CAGCGGCAAT TTGATATCCA ACACAAAGGA ATGAACCCCT GCCCCATGGT CCTGGTCTTC
    GGGTGCCGGC AATCCAAGAT AGATCATATC TACAGGGAAG AGACCCTGCA GGCCAAGAAC
    AAGGGGGTCT TCAGAGAGCT GTACACGGCT TACTCCCGGG AGCCAGACAA ACCAAAGAAG
    TATGTGCAGG ACATCCTGCA GGAGCAGCTG GCGGAGTCTG TGTACCGAGC CCTGAAGGAG
    CAAGGGGGCC ACATATACGT CTGTGGGGAC GTCACCATGG CTGCTGATGT CCTCAAAGCC
    ATCCAGCGCA TCATGACCCA GCAGGGGAAG CTCTCGGCAG AGGACGCCGG CGTATTCATC
    AGCCGGATGA GGGATGACAA CCGATACCAT GAGGATATTT TTGGAGTCAC CCTGCGAACG
    TACGAAGTGA CCAACCGCCT TAGATCTGAG TCCATTGCCT TCATTGAAGA GAGCAAAAAA
    GACACCGATG AGGTTTTCAG CTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016779820.1
    CDS2462..6868
    Translation

    Target ORF information:

    RefSeq Version XM_016924331.1
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes nitric oxide synthase 1 (NOS1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_016924331.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAGGATC ACATGTTCGG TGTTCAGCAA ATCCAGCCCA ATGTCATTTC TGTTCGTCTC 
    TTCAAGCGCA AAGTTGGGGG CCTGGGATTC CTGGTGAAGG AGCGGGTCAG TAAGCCGCCC
    GTGATTATCT CTGACCTGAT TCGTGGGGGC GCCGCAGAGC AGAGTGGCCT CATCCAGGCC
    GGAGACATCA TTCTTGCGGT CAACGGCCGG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC
    CTGGAGGTAC TCAGAGGCAT TGCCTCCGAG ACCCACGTGG TCCTCATTCT GAGGGGTCCT
    GAAGGTTTCA CCACGCACCT GGAGACCACC TTTACAGGTG ATGGGACCCC CAAGACCATC
    CGGGTGACAC AGCCCCTGGG TCCCCCCACC AAAGCCGTGG ATCTGTCCCA CCAGCCATCG
    GCCGGCAAAG AACAGCCGCT GGCAGTGGAT GGGGCCTCGG GTCCCGGGAA TGGGCCTCAG
    CATGCCTACG ATGACGGGCA GGAGGCTGGC TCACTCCCCC ATGCCAACGG CCTGGCCCCC
    AGGCCCCCAG GCCAGGACCC TGCGAAGAAA GCAACCAGAG TCAGCCTCCA AGGCAGAGGG
    GAGAACAATG AACTGCTCAA GGAGATAGAG CCTGTGCTGA GCCTTCTCAC CAGTGGGAGC
    AGAGGGGTCA AGGGAGGGGC ACCTGCCAAG GCAGAGATGA AAGATATGGG AATCCAGGTG
    GACAGAGATT TGGACGGCAA GTCACACAAA CCTCTGCCCC TCGCCGTGGA GAATGACCGA
    GTCTTCAATG ACCTATGGGG GAAGGGCAAT GTGCCTGTCG TCCTCAACAA CCCATATTCA
    GAGAAGGAGC AGCCCCCTGC CTCAGGAAAA CAGTCCCCCA CAAAGAATGG CAGCCCCTCC
    AAGTGTCCAC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG AGGTGGTTCT CACTGACACC
    CTCCACCTTA AGAGCACATT GGAAACGGGA TGCACTGAGT ACATCTGCAT GGGCTCCATC
    ATGCATCCTT CTCAGCATGC AAGGAGGCCT GAAGATGTCC GCACAAAAGA ACAGCTCTTC
    CCTCTCGCCA AAGAGTTTAT TGATCAATAC TATTCATCAA TTAAAAGATT TGGCTCCAAA
    GCCCACATGG AAAGGCTGGA AGAGGTGAAC AAAGAGATCG AAACCACTAG CACCTACCAG
    CTCAAGGACA CAGAGCTCAT CTATGGGGCC AAGCACGCCT GGCGGAATGC CTCGCGCTGT
    GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTATTCGATG CCCGTGACTG CACCACGGCC
    CACGGGATGT TCAACTACAT CTGTAACCAC GTCAAGTATG CCACCAACAA AGGGAACCTC
    AGGTCCGCCA TCACCATATT CCCCCAGAGG ACAGACGGCA AGCACGACTT CCGAGTCTGG
    AACTCCCAGC TCATCCGCTA CGCTGGCTAC AAGCAGCCCG ACGGCTCCAC CCTGGGGGAC
    CCAGCCAATG TGCAGTTCAC AGAGATATGC ATACAGCAGG GCTGGAAACC GCCTAGAGGC
    CGCTTCGATG TCCTGCCGCT CCTGCTTCAG GCCAACGGCA ATGACCCTGA GCTCTTCCAG
    ATTCCTCCAG AGCTGGTGTT GGAAGTTCCC ATCAGGCACC CCAAGTTTGA GTGGTTCAAG
    GACCTGGGGC TGAAGTGGTA CGGCCTCCCC GCCGTGTCCA ACATGCTCCT AGAGATTGGC
    GGCCTGGAGT TCAGCGCCTG TCCCTTCAGT GGCTGGTACA TGGGCACAGA GATTGGTGTC
    CGCGACTACT GTGACAACTC CCGCTACAAT ATCCTGGAGG AAGTGGCCAA GAAGATGAAC
    TTAGACATGA GGAAGATGTC CTCCCTGTGG AAGGACCAGG CGCTGGTGGA GATCAATATC
    GCGGTTCTCT ACAGCTTCCA GAGTGACAAA GTGACCATTG TTGACCATCA CTCCGCCACC
    GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGGGGCTG CCCTGCCGAC
    TGGGTGTGGA TCGTGCCCCC CATGTCCGGA AGCATCACCC CTGTGTTCCA CCAGGAGATG
    CTCAACTACC GGCTCACCCC CTCCTTCGAA TACCAGCCTG ATCCCTGGAA CACGCATGTC
    TGGAAAGGCA CCAACGGGAC CCCCACAAAG CGGCGAGCCA TCGGCTTCAA GAAGCTAGCA
    GAAGCTGTCA AGTTCTCGGC CAAGCTGATG GGGCAGGCTA TGGCCAAGAG GGTCAAAGCG
    ACCATCCTCT ATGCCACAGA GACAGGCAAA TCGCAAGCTT ATGCCAAGAC CTTGTGTGAG
    ATCTTCAAAC ACGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATCGTGCAC
    CTGGAACATG AAACTCTGGT CCTCGTGGTC ACCAGCACCT TTGGCAATGG AGATCCCCCT
    GAGAATGGGG AGAAATTCGG CTGTGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAG
    GAAGAAAGGA AGTACCCGGA ACCCTTGCGT TTCTTTCCCC GTAAAGGGCC TCCCCTCCCC
    AATGGTGACA CAGAAGTCCA CGGTCTGGCT GCAGCCCGTG ACAGCCAGCA CAGGAGCTAC
    AAGGTCCGAT TCAACAGCGT CTCCTCCTAC TCTGACTCCC AAAAATCATC AGGCGATGGG
    CCCGACCTCA GAGACAACTT TGAGAGTGCT GGACCCCTGG CCAATGTGAG GTTCTCAGTT
    TTTGGCCTCG GCTCACGAGC ATACCCTCAC TTTTGTGCCT TCGGACACGC TGTGGACACC
    CTCCTGGAAG AACTGGGAGG GGAGAGGATC CTGAAGATGA GGGAAGGGGA TGAGCTCTGT
    GGGCAGGAAG AGGCTTTCAG GACCTGGGCC AAGAAGGTCT TCAAGGCAGC CTGTGATGTC
    TTCTGTGTGG GAGATGATGT CAACATTGAA AAGGCCAACA ATTCCCTCAT CAGCAATGAT
    CGCAGCTGGA AGAGAAACAA GTTCCGCCTC ACCTTTGTGG CCGAAGCTCC AGAACTCACA
    CAAGGTCTAT CCAATGTCCA CAAAAAGCGA GTCTCAGCTG CCCGGCTCCT TAGCCGTCAA
    AACCTCCAGA GTCCTAAATC CAGTCGGTCA ACTATCTTCG TGCGTCTCCA CACCAACGGG
    AACCAGGAGC TGCAGTACCA GCCTGGGGAC CACCTGGGTG TCTTCCCTGG CAACCACGAG
    GACCTCGTGA ATGCCCTGAT CGAGCGGCTG GAGGACGCGC CCCCTGTCAA CCAGATGGTG
    AAAGTGGAAC TGCTGGAGGA GCGGAACACG GCTTTAGGTG TCATCAGTAA CTGGACAGAC
    GAGCTCCGCC TCCCGCCCTG CACCATCTTC CAGGCCTTCA AGTACTACCT GGACATCACC
    ACGCCACCAA CGCCCCTGCA GCTGCAGCAG TTTGCCTCCC TAGCTACCAG CGAGAAGGAG
    AAGCAGCGTC TGCTGGTCCT CAGCAAGGGT TTGCAGGAGT ACGAGGAATG GAAATGGGGC
    AAGAACCCCA CCATCGTGGA GGTGCTGGAG GAATTCCCAT CTATCCAGAT GCCGGCCACC
    CTGCTCCTGA CCCAGCTGTC CCTGCTGCAG CCCCGCTACT ATTCCATCAG CTCCTCCCCA
    GACATGTACC CTGATGAAGT GCACCTCACT GTGGCCATCG TTTCCTACCG CACTCGAGAT
    GGAGAAGGAC CAATTCACCA CGGCGTATGC TCCTCCTGGC TCAACCGGAT ACAGGCTGAC
    GAACTGGTCC CCTGTTTCGT GAGAGGAGCA CCCAGCTTCC ACCTGCCCCG GAACCCCCAA
    GTCCCCTGCA TCCTCGTTGG ACCAGGCACC GGCATTGCCC CTTTCCGAAG CTTCTGGCAA
    CAGCGGCAAT TTGATATCCA ACACAAAGGA ATGAACCCCT GCCCCATGGT CCTGGTCTTC
    GGGTGCCGGC AATCCAAGAT AGATCATATC TACAGGGAAG AGACCCTGCA GGCCAAGAAC
    AAGGGGGTCT TCAGAGAGCT GTACACGGCT TACTCCCGGG AGCCAGACAA ACCAAAGAAG
    TATGTGCAGG ACATCCTGCA GGAGCAGCTG GCGGAGTCTG TGTACCGAGC CCTGAAGGAG
    CAAGGGGGCC ACATATACGT CTGTGGGGAC GTCACCATGG CTGCTGATGT CCTCAAAGCC
    ATCCAGCGCA TCATGACCCA GCAGGGGAAG CTCTCGGCAG AGGACGCCGG CGTATTCATC
    AGCCGGATGA GGGATGACAA CCGATACCAT GAGGATATTT TTGGAGTCAC CCTGCGAACG
    TACGAAGTGA CCAACCGCCT TAGATCTGAG TCCATTGCCT TCATTGAAGA GAGCAAAAAA
    GACACCGATG AGGTTTTCAG CTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc66615
    Clone ID Related Accession (Same CDS sequence) XM_016924331.1 , XM_016924327.2 , XM_016924331.2 , XM_016924328.1 , XM_016924332.2 , XM_016924327.1 , XM_016924332.1 , XM_016924328.2 , XM_016924329.2 , XM_016924329.1
    Accession Version XM_016924327.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4407bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-19
    Organism Pan troglodytes(chimpanzee)
    Product nitric oxide synthase, brain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036891.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016924327.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAGGATC ACATGTTCGG TGTTCAGCAA ATCCAGCCCA ATGTCATTTC TGTTCGTCTC 
    TTCAAGCGCA AAGTTGGGGG CCTGGGATTC CTGGTGAAGG AGCGGGTCAG TAAGCCGCCC
    GTGATTATCT CTGACCTGAT TCGTGGGGGC GCCGCAGAGC AGAGTGGCCT CATCCAGGCC
    GGAGACATCA TTCTTGCGGT CAACGGCCGG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC
    CTGGAGGTAC TCAGAGGCAT TGCCTCCGAG ACCCACGTGG TCCTCATTCT GAGGGGTCCT
    GAAGGTTTCA CCACGCACCT GGAGACCACC TTTACAGGTG ATGGGACCCC CAAGACCATC
    CGGGTGACAC AGCCCCTGGG TCCCCCCACC AAAGCCGTGG ATCTGTCCCA CCAGCCATCG
    GCCGGCAAAG AACAGCCGCT GGCAGTGGAT GGGGCCTCGG GTCCCGGGAA TGGGCCTCAG
    CATGCCTACG ATGACGGGCA GGAGGCTGGC TCACTCCCCC ATGCCAACGG CCTGGCCCCC
    AGGCCCCCAG GCCAGGACCC TGCGAAGAAA GCAACCAGAG TCAGCCTCCA AGGCAGAGGG
    GAGAACAATG AACTGCTCAA GGAGATAGAG CCTGTGCTGA GCCTTCTCAC CAGTGGGAGC
    AGAGGGGTCA AGGGAGGGGC ACCTGCCAAG GCAGAGATGA AAGATATGGG AATCCAGGTG
    GACAGAGATT TGGACGGCAA GTCACACAAA CCTCTGCCCC TCGCCGTGGA GAATGACCGA
    GTCTTCAATG ACCTATGGGG GAAGGGCAAT GTGCCTGTCG TCCTCAACAA CCCATATTCA
    GAGAAGGAGC AGCCCCCTGC CTCAGGAAAA CAGTCCCCCA CAAAGAATGG CAGCCCCTCC
    AAGTGTCCAC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG AGGTGGTTCT CACTGACACC
    CTCCACCTTA AGAGCACATT GGAAACGGGA TGCACTGAGT ACATCTGCAT GGGCTCCATC
    ATGCATCCTT CTCAGCATGC AAGGAGGCCT GAAGATGTCC GCACAAAAGA ACAGCTCTTC
    CCTCTCGCCA AAGAGTTTAT TGATCAATAC TATTCATCAA TTAAAAGATT TGGCTCCAAA
    GCCCACATGG AAAGGCTGGA AGAGGTGAAC AAAGAGATCG AAACCACTAG CACCTACCAG
    CTCAAGGACA CAGAGCTCAT CTATGGGGCC AAGCACGCCT GGCGGAATGC CTCGCGCTGT
    GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTATTCGATG CCCGTGACTG CACCACGGCC
    CACGGGATGT TCAACTACAT CTGTAACCAC GTCAAGTATG CCACCAACAA AGGGAACCTC
    AGGTCCGCCA TCACCATATT CCCCCAGAGG ACAGACGGCA AGCACGACTT CCGAGTCTGG
    AACTCCCAGC TCATCCGCTA CGCTGGCTAC AAGCAGCCCG ACGGCTCCAC CCTGGGGGAC
    CCAGCCAATG TGCAGTTCAC AGAGATATGC ATACAGCAGG GCTGGAAACC GCCTAGAGGC
    CGCTTCGATG TCCTGCCGCT CCTGCTTCAG GCCAACGGCA ATGACCCTGA GCTCTTCCAG
    ATTCCTCCAG AGCTGGTGTT GGAAGTTCCC ATCAGGCACC CCAAGTTTGA GTGGTTCAAG
    GACCTGGGGC TGAAGTGGTA CGGCCTCCCC GCCGTGTCCA ACATGCTCCT AGAGATTGGC
    GGCCTGGAGT TCAGCGCCTG TCCCTTCAGT GGCTGGTACA TGGGCACAGA GATTGGTGTC
    CGCGACTACT GTGACAACTC CCGCTACAAT ATCCTGGAGG AAGTGGCCAA GAAGATGAAC
    TTAGACATGA GGAAGATGTC CTCCCTGTGG AAGGACCAGG CGCTGGTGGA GATCAATATC
    GCGGTTCTCT ACAGCTTCCA GAGTGACAAA GTGACCATTG TTGACCATCA CTCCGCCACC
    GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGGGGCTG CCCTGCCGAC
    TGGGTGTGGA TCGTGCCCCC CATGTCCGGA AGCATCACCC CTGTGTTCCA CCAGGAGATG
    CTCAACTACC GGCTCACCCC CTCCTTCGAA TACCAGCCTG ATCCCTGGAA CACGCATGTC
    TGGAAAGGCA CCAACGGGAC CCCCACAAAG CGGCGAGCCA TCGGCTTCAA GAAGCTAGCA
    GAAGCTGTCA AGTTCTCGGC CAAGCTGATG GGGCAGGCTA TGGCCAAGAG GGTCAAAGCG
    ACCATCCTCT ATGCCACAGA GACAGGCAAA TCGCAAGCTT ATGCCAAGAC CTTGTGTGAG
    ATCTTCAAAC ACGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATCGTGCAC
    CTGGAACATG AAACTCTGGT CCTCGTGGTC ACCAGCACCT TTGGCAATGG AGATCCCCCT
    GAGAATGGGG AGAAATTCGG CTGTGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAG
    GAAGAAAGGA AGTACCCGGA ACCCTTGCGT TTCTTTCCCC GTAAAGGGCC TCCCCTCCCC
    AATGGTGACA CAGAAGTCCA CGGTCTGGCT GCAGCCCGTG ACAGCCAGCA CAGGAGCTAC
    AAGGTCCGAT TCAACAGCGT CTCCTCCTAC TCTGACTCCC AAAAATCATC AGGCGATGGG
    CCCGACCTCA GAGACAACTT TGAGAGTGCT GGACCCCTGG CCAATGTGAG GTTCTCAGTT
    TTTGGCCTCG GCTCACGAGC ATACCCTCAC TTTTGTGCCT TCGGACACGC TGTGGACACC
    CTCCTGGAAG AACTGGGAGG GGAGAGGATC CTGAAGATGA GGGAAGGGGA TGAGCTCTGT
    GGGCAGGAAG AGGCTTTCAG GACCTGGGCC AAGAAGGTCT TCAAGGCAGC CTGTGATGTC
    TTCTGTGTGG GAGATGATGT CAACATTGAA AAGGCCAACA ATTCCCTCAT CAGCAATGAT
    CGCAGCTGGA AGAGAAACAA GTTCCGCCTC ACCTTTGTGG CCGAAGCTCC AGAACTCACA
    CAAGGTCTAT CCAATGTCCA CAAAAAGCGA GTCTCAGCTG CCCGGCTCCT TAGCCGTCAA
    AACCTCCAGA GTCCTAAATC CAGTCGGTCA ACTATCTTCG TGCGTCTCCA CACCAACGGG
    AACCAGGAGC TGCAGTACCA GCCTGGGGAC CACCTGGGTG TCTTCCCTGG CAACCACGAG
    GACCTCGTGA ATGCCCTGAT CGAGCGGCTG GAGGACGCGC CCCCTGTCAA CCAGATGGTG
    AAAGTGGAAC TGCTGGAGGA GCGGAACACG GCTTTAGGTG TCATCAGTAA CTGGACAGAC
    GAGCTCCGCC TCCCGCCCTG CACCATCTTC CAGGCCTTCA AGTACTACCT GGACATCACC
    ACGCCACCAA CGCCCCTGCA GCTGCAGCAG TTTGCCTCCC TAGCTACCAG CGAGAAGGAG
    AAGCAGCGTC TGCTGGTCCT CAGCAAGGGT TTGCAGGAGT ACGAGGAATG GAAATGGGGC
    AAGAACCCCA CCATCGTGGA GGTGCTGGAG GAATTCCCAT CTATCCAGAT GCCGGCCACC
    CTGCTCCTGA CCCAGCTGTC CCTGCTGCAG CCCCGCTACT ATTCCATCAG CTCCTCCCCA
    GACATGTACC CTGATGAAGT GCACCTCACT GTGGCCATCG TTTCCTACCG CACTCGAGAT
    GGAGAAGGAC CAATTCACCA CGGCGTATGC TCCTCCTGGC TCAACCGGAT ACAGGCTGAC
    GAACTGGTCC CCTGTTTCGT GAGAGGAGCA CCCAGCTTCC ACCTGCCCCG GAACCCCCAA
    GTCCCCTGCA TCCTCGTTGG ACCAGGCACC GGCATTGCCC CTTTCCGAAG CTTCTGGCAA
    CAGCGGCAAT TTGATATCCA ACACAAAGGA ATGAACCCCT GCCCCATGGT CCTGGTCTTC
    GGGTGCCGGC AATCCAAGAT AGATCATATC TACAGGGAAG AGACCCTGCA GGCCAAGAAC
    AAGGGGGTCT TCAGAGAGCT GTACACGGCT TACTCCCGGG AGCCAGACAA ACCAAAGAAG
    TATGTGCAGG ACATCCTGCA GGAGCAGCTG GCGGAGTCTG TGTACCGAGC CCTGAAGGAG
    CAAGGGGGCC ACATATACGT CTGTGGGGAC GTCACCATGG CTGCTGATGT CCTCAAAGCC
    ATCCAGCGCA TCATGACCCA GCAGGGGAAG CTCTCGGCAG AGGACGCCGG CGTATTCATC
    AGCCGGATGA GGGATGACAA CCGATACCAT GAGGATATTT TTGGAGTCAC CCTGCGAACG
    TACGAAGTGA CCAACCGCCT TAGATCTGAG TCCATTGCCT TCATTGAAGA GAGCAAAAAA
    GACACCGATG AGGTTTTCAG CTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016779816.1
    CDS2028..6434
    Translation

    Target ORF information:

    RefSeq Version XM_016924327.2
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes nitric oxide synthase 1 (NOS1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_016924327.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAGGATC ACATGTTCGG TGTTCAGCAA ATCCAGCCCA ATGTCATTTC TGTTCGTCTC 
    TTCAAGCGCA AAGTTGGGGG CCTGGGATTC CTGGTGAAGG AGCGGGTCAG TAAGCCGCCC
    GTGATTATCT CTGACCTGAT TCGTGGGGGC GCCGCAGAGC AGAGTGGCCT CATCCAGGCC
    GGAGACATCA TTCTTGCGGT CAACGGCCGG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC
    CTGGAGGTAC TCAGAGGCAT TGCCTCCGAG ACCCACGTGG TCCTCATTCT GAGGGGTCCT
    GAAGGTTTCA CCACGCACCT GGAGACCACC TTTACAGGTG ATGGGACCCC CAAGACCATC
    CGGGTGACAC AGCCCCTGGG TCCCCCCACC AAAGCCGTGG ATCTGTCCCA CCAGCCATCG
    GCCGGCAAAG AACAGCCGCT GGCAGTGGAT GGGGCCTCGG GTCCCGGGAA TGGGCCTCAG
    CATGCCTACG ATGACGGGCA GGAGGCTGGC TCACTCCCCC ATGCCAACGG CCTGGCCCCC
    AGGCCCCCAG GCCAGGACCC TGCGAAGAAA GCAACCAGAG TCAGCCTCCA AGGCAGAGGG
    GAGAACAATG AACTGCTCAA GGAGATAGAG CCTGTGCTGA GCCTTCTCAC CAGTGGGAGC
    AGAGGGGTCA AGGGAGGGGC ACCTGCCAAG GCAGAGATGA AAGATATGGG AATCCAGGTG
    GACAGAGATT TGGACGGCAA GTCACACAAA CCTCTGCCCC TCGCCGTGGA GAATGACCGA
    GTCTTCAATG ACCTATGGGG GAAGGGCAAT GTGCCTGTCG TCCTCAACAA CCCATATTCA
    GAGAAGGAGC AGCCCCCTGC CTCAGGAAAA CAGTCCCCCA CAAAGAATGG CAGCCCCTCC
    AAGTGTCCAC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG AGGTGGTTCT CACTGACACC
    CTCCACCTTA AGAGCACATT GGAAACGGGA TGCACTGAGT ACATCTGCAT GGGCTCCATC
    ATGCATCCTT CTCAGCATGC AAGGAGGCCT GAAGATGTCC GCACAAAAGA ACAGCTCTTC
    CCTCTCGCCA AAGAGTTTAT TGATCAATAC TATTCATCAA TTAAAAGATT TGGCTCCAAA
    GCCCACATGG AAAGGCTGGA AGAGGTGAAC AAAGAGATCG AAACCACTAG CACCTACCAG
    CTCAAGGACA CAGAGCTCAT CTATGGGGCC AAGCACGCCT GGCGGAATGC CTCGCGCTGT
    GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTATTCGATG CCCGTGACTG CACCACGGCC
    CACGGGATGT TCAACTACAT CTGTAACCAC GTCAAGTATG CCACCAACAA AGGGAACCTC
    AGGTCCGCCA TCACCATATT CCCCCAGAGG ACAGACGGCA AGCACGACTT CCGAGTCTGG
    AACTCCCAGC TCATCCGCTA CGCTGGCTAC AAGCAGCCCG ACGGCTCCAC CCTGGGGGAC
    CCAGCCAATG TGCAGTTCAC AGAGATATGC ATACAGCAGG GCTGGAAACC GCCTAGAGGC
    CGCTTCGATG TCCTGCCGCT CCTGCTTCAG GCCAACGGCA ATGACCCTGA GCTCTTCCAG
    ATTCCTCCAG AGCTGGTGTT GGAAGTTCCC ATCAGGCACC CCAAGTTTGA GTGGTTCAAG
    GACCTGGGGC TGAAGTGGTA CGGCCTCCCC GCCGTGTCCA ACATGCTCCT AGAGATTGGC
    GGCCTGGAGT TCAGCGCCTG TCCCTTCAGT GGCTGGTACA TGGGCACAGA GATTGGTGTC
    CGCGACTACT GTGACAACTC CCGCTACAAT ATCCTGGAGG AAGTGGCCAA GAAGATGAAC
    TTAGACATGA GGAAGATGTC CTCCCTGTGG AAGGACCAGG CGCTGGTGGA GATCAATATC
    GCGGTTCTCT ACAGCTTCCA GAGTGACAAA GTGACCATTG TTGACCATCA CTCCGCCACC
    GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGGGGCTG CCCTGCCGAC
    TGGGTGTGGA TCGTGCCCCC CATGTCCGGA AGCATCACCC CTGTGTTCCA CCAGGAGATG
    CTCAACTACC GGCTCACCCC CTCCTTCGAA TACCAGCCTG ATCCCTGGAA CACGCATGTC
    TGGAAAGGCA CCAACGGGAC CCCCACAAAG CGGCGAGCCA TCGGCTTCAA GAAGCTAGCA
    GAAGCTGTCA AGTTCTCGGC CAAGCTGATG GGGCAGGCTA TGGCCAAGAG GGTCAAAGCG
    ACCATCCTCT ATGCCACAGA GACAGGCAAA TCGCAAGCTT ATGCCAAGAC CTTGTGTGAG
    ATCTTCAAAC ACGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATCGTGCAC
    CTGGAACATG AAACTCTGGT CCTCGTGGTC ACCAGCACCT TTGGCAATGG AGATCCCCCT
    GAGAATGGGG AGAAATTCGG CTGTGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAG
    GAAGAAAGGA AGTACCCGGA ACCCTTGCGT TTCTTTCCCC GTAAAGGGCC TCCCCTCCCC
    AATGGTGACA CAGAAGTCCA CGGTCTGGCT GCAGCCCGTG ACAGCCAGCA CAGGAGCTAC
    AAGGTCCGAT TCAACAGCGT CTCCTCCTAC TCTGACTCCC AAAAATCATC AGGCGATGGG
    CCCGACCTCA GAGACAACTT TGAGAGTGCT GGACCCCTGG CCAATGTGAG GTTCTCAGTT
    TTTGGCCTCG GCTCACGAGC ATACCCTCAC TTTTGTGCCT TCGGACACGC TGTGGACACC
    CTCCTGGAAG AACTGGGAGG GGAGAGGATC CTGAAGATGA GGGAAGGGGA TGAGCTCTGT
    GGGCAGGAAG AGGCTTTCAG GACCTGGGCC AAGAAGGTCT TCAAGGCAGC CTGTGATGTC
    TTCTGTGTGG GAGATGATGT CAACATTGAA AAGGCCAACA ATTCCCTCAT CAGCAATGAT
    CGCAGCTGGA AGAGAAACAA GTTCCGCCTC ACCTTTGTGG CCGAAGCTCC AGAACTCACA
    CAAGGTCTAT CCAATGTCCA CAAAAAGCGA GTCTCAGCTG CCCGGCTCCT TAGCCGTCAA
    AACCTCCAGA GTCCTAAATC CAGTCGGTCA ACTATCTTCG TGCGTCTCCA CACCAACGGG
    AACCAGGAGC TGCAGTACCA GCCTGGGGAC CACCTGGGTG TCTTCCCTGG CAACCACGAG
    GACCTCGTGA ATGCCCTGAT CGAGCGGCTG GAGGACGCGC CCCCTGTCAA CCAGATGGTG
    AAAGTGGAAC TGCTGGAGGA GCGGAACACG GCTTTAGGTG TCATCAGTAA CTGGACAGAC
    GAGCTCCGCC TCCCGCCCTG CACCATCTTC CAGGCCTTCA AGTACTACCT GGACATCACC
    ACGCCACCAA CGCCCCTGCA GCTGCAGCAG TTTGCCTCCC TAGCTACCAG CGAGAAGGAG
    AAGCAGCGTC TGCTGGTCCT CAGCAAGGGT TTGCAGGAGT ACGAGGAATG GAAATGGGGC
    AAGAACCCCA CCATCGTGGA GGTGCTGGAG GAATTCCCAT CTATCCAGAT GCCGGCCACC
    CTGCTCCTGA CCCAGCTGTC CCTGCTGCAG CCCCGCTACT ATTCCATCAG CTCCTCCCCA
    GACATGTACC CTGATGAAGT GCACCTCACT GTGGCCATCG TTTCCTACCG CACTCGAGAT
    GGAGAAGGAC CAATTCACCA CGGCGTATGC TCCTCCTGGC TCAACCGGAT ACAGGCTGAC
    GAACTGGTCC CCTGTTTCGT GAGAGGAGCA CCCAGCTTCC ACCTGCCCCG GAACCCCCAA
    GTCCCCTGCA TCCTCGTTGG ACCAGGCACC GGCATTGCCC CTTTCCGAAG CTTCTGGCAA
    CAGCGGCAAT TTGATATCCA ACACAAAGGA ATGAACCCCT GCCCCATGGT CCTGGTCTTC
    GGGTGCCGGC AATCCAAGAT AGATCATATC TACAGGGAAG AGACCCTGCA GGCCAAGAAC
    AAGGGGGTCT TCAGAGAGCT GTACACGGCT TACTCCCGGG AGCCAGACAA ACCAAAGAAG
    TATGTGCAGG ACATCCTGCA GGAGCAGCTG GCGGAGTCTG TGTACCGAGC CCTGAAGGAG
    CAAGGGGGCC ACATATACGT CTGTGGGGAC GTCACCATGG CTGCTGATGT CCTCAAAGCC
    ATCCAGCGCA TCATGACCCA GCAGGGGAAG CTCTCGGCAG AGGACGCCGG CGTATTCATC
    AGCCGGATGA GGGATGACAA CCGATACCAT GAGGATATTT TTGGAGTCAC CCTGCGAACG
    TACGAAGTGA CCAACCGCCT TAGATCTGAG TCCATTGCCT TCATTGAAGA GAGCAAAAAA
    GACACCGATG AGGTTTTCAG CTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc66615
    Clone ID Related Accession (Same CDS sequence) XM_016924331.1 , XM_016924327.2 , XM_016924331.2 , XM_016924328.1 , XM_016924332.2 , XM_016924327.1 , XM_016924332.1 , XM_016924328.2 , XM_016924329.2 , XM_016924329.1
    Accession Version XM_016924331.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4407bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-19
    Organism Pan troglodytes(chimpanzee)
    Product nitric oxide synthase, brain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036891.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016924331.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAGGATC ACATGTTCGG TGTTCAGCAA ATCCAGCCCA ATGTCATTTC TGTTCGTCTC 
    TTCAAGCGCA AAGTTGGGGG CCTGGGATTC CTGGTGAAGG AGCGGGTCAG TAAGCCGCCC
    GTGATTATCT CTGACCTGAT TCGTGGGGGC GCCGCAGAGC AGAGTGGCCT CATCCAGGCC
    GGAGACATCA TTCTTGCGGT CAACGGCCGG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC
    CTGGAGGTAC TCAGAGGCAT TGCCTCCGAG ACCCACGTGG TCCTCATTCT GAGGGGTCCT
    GAAGGTTTCA CCACGCACCT GGAGACCACC TTTACAGGTG ATGGGACCCC CAAGACCATC
    CGGGTGACAC AGCCCCTGGG TCCCCCCACC AAAGCCGTGG ATCTGTCCCA CCAGCCATCG
    GCCGGCAAAG AACAGCCGCT GGCAGTGGAT GGGGCCTCGG GTCCCGGGAA TGGGCCTCAG
    CATGCCTACG ATGACGGGCA GGAGGCTGGC TCACTCCCCC ATGCCAACGG CCTGGCCCCC
    AGGCCCCCAG GCCAGGACCC TGCGAAGAAA GCAACCAGAG TCAGCCTCCA AGGCAGAGGG
    GAGAACAATG AACTGCTCAA GGAGATAGAG CCTGTGCTGA GCCTTCTCAC CAGTGGGAGC
    AGAGGGGTCA AGGGAGGGGC ACCTGCCAAG GCAGAGATGA AAGATATGGG AATCCAGGTG
    GACAGAGATT TGGACGGCAA GTCACACAAA CCTCTGCCCC TCGCCGTGGA GAATGACCGA
    GTCTTCAATG ACCTATGGGG GAAGGGCAAT GTGCCTGTCG TCCTCAACAA CCCATATTCA
    GAGAAGGAGC AGCCCCCTGC CTCAGGAAAA CAGTCCCCCA CAAAGAATGG CAGCCCCTCC
    AAGTGTCCAC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG AGGTGGTTCT CACTGACACC
    CTCCACCTTA AGAGCACATT GGAAACGGGA TGCACTGAGT ACATCTGCAT GGGCTCCATC
    ATGCATCCTT CTCAGCATGC AAGGAGGCCT GAAGATGTCC GCACAAAAGA ACAGCTCTTC
    CCTCTCGCCA AAGAGTTTAT TGATCAATAC TATTCATCAA TTAAAAGATT TGGCTCCAAA
    GCCCACATGG AAAGGCTGGA AGAGGTGAAC AAAGAGATCG AAACCACTAG CACCTACCAG
    CTCAAGGACA CAGAGCTCAT CTATGGGGCC AAGCACGCCT GGCGGAATGC CTCGCGCTGT
    GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTATTCGATG CCCGTGACTG CACCACGGCC
    CACGGGATGT TCAACTACAT CTGTAACCAC GTCAAGTATG CCACCAACAA AGGGAACCTC
    AGGTCCGCCA TCACCATATT CCCCCAGAGG ACAGACGGCA AGCACGACTT CCGAGTCTGG
    AACTCCCAGC TCATCCGCTA CGCTGGCTAC AAGCAGCCCG ACGGCTCCAC CCTGGGGGAC
    CCAGCCAATG TGCAGTTCAC AGAGATATGC ATACAGCAGG GCTGGAAACC GCCTAGAGGC
    CGCTTCGATG TCCTGCCGCT CCTGCTTCAG GCCAACGGCA ATGACCCTGA GCTCTTCCAG
    ATTCCTCCAG AGCTGGTGTT GGAAGTTCCC ATCAGGCACC CCAAGTTTGA GTGGTTCAAG
    GACCTGGGGC TGAAGTGGTA CGGCCTCCCC GCCGTGTCCA ACATGCTCCT AGAGATTGGC
    GGCCTGGAGT TCAGCGCCTG TCCCTTCAGT GGCTGGTACA TGGGCACAGA GATTGGTGTC
    CGCGACTACT GTGACAACTC CCGCTACAAT ATCCTGGAGG AAGTGGCCAA GAAGATGAAC
    TTAGACATGA GGAAGATGTC CTCCCTGTGG AAGGACCAGG CGCTGGTGGA GATCAATATC
    GCGGTTCTCT ACAGCTTCCA GAGTGACAAA GTGACCATTG TTGACCATCA CTCCGCCACC
    GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGGGGCTG CCCTGCCGAC
    TGGGTGTGGA TCGTGCCCCC CATGTCCGGA AGCATCACCC CTGTGTTCCA CCAGGAGATG
    CTCAACTACC GGCTCACCCC CTCCTTCGAA TACCAGCCTG ATCCCTGGAA CACGCATGTC
    TGGAAAGGCA CCAACGGGAC CCCCACAAAG CGGCGAGCCA TCGGCTTCAA GAAGCTAGCA
    GAAGCTGTCA AGTTCTCGGC CAAGCTGATG GGGCAGGCTA TGGCCAAGAG GGTCAAAGCG
    ACCATCCTCT ATGCCACAGA GACAGGCAAA TCGCAAGCTT ATGCCAAGAC CTTGTGTGAG
    ATCTTCAAAC ACGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATCGTGCAC
    CTGGAACATG AAACTCTGGT CCTCGTGGTC ACCAGCACCT TTGGCAATGG AGATCCCCCT
    GAGAATGGGG AGAAATTCGG CTGTGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAG
    GAAGAAAGGA AGTACCCGGA ACCCTTGCGT TTCTTTCCCC GTAAAGGGCC TCCCCTCCCC
    AATGGTGACA CAGAAGTCCA CGGTCTGGCT GCAGCCCGTG ACAGCCAGCA CAGGAGCTAC
    AAGGTCCGAT TCAACAGCGT CTCCTCCTAC TCTGACTCCC AAAAATCATC AGGCGATGGG
    CCCGACCTCA GAGACAACTT TGAGAGTGCT GGACCCCTGG CCAATGTGAG GTTCTCAGTT
    TTTGGCCTCG GCTCACGAGC ATACCCTCAC TTTTGTGCCT TCGGACACGC TGTGGACACC
    CTCCTGGAAG AACTGGGAGG GGAGAGGATC CTGAAGATGA GGGAAGGGGA TGAGCTCTGT
    GGGCAGGAAG AGGCTTTCAG GACCTGGGCC AAGAAGGTCT TCAAGGCAGC CTGTGATGTC
    TTCTGTGTGG GAGATGATGT CAACATTGAA AAGGCCAACA ATTCCCTCAT CAGCAATGAT
    CGCAGCTGGA AGAGAAACAA GTTCCGCCTC ACCTTTGTGG CCGAAGCTCC AGAACTCACA
    CAAGGTCTAT CCAATGTCCA CAAAAAGCGA GTCTCAGCTG CCCGGCTCCT TAGCCGTCAA
    AACCTCCAGA GTCCTAAATC CAGTCGGTCA ACTATCTTCG TGCGTCTCCA CACCAACGGG
    AACCAGGAGC TGCAGTACCA GCCTGGGGAC CACCTGGGTG TCTTCCCTGG CAACCACGAG
    GACCTCGTGA ATGCCCTGAT CGAGCGGCTG GAGGACGCGC CCCCTGTCAA CCAGATGGTG
    AAAGTGGAAC TGCTGGAGGA GCGGAACACG GCTTTAGGTG TCATCAGTAA CTGGACAGAC
    GAGCTCCGCC TCCCGCCCTG CACCATCTTC CAGGCCTTCA AGTACTACCT GGACATCACC
    ACGCCACCAA CGCCCCTGCA GCTGCAGCAG TTTGCCTCCC TAGCTACCAG CGAGAAGGAG
    AAGCAGCGTC TGCTGGTCCT CAGCAAGGGT TTGCAGGAGT ACGAGGAATG GAAATGGGGC
    AAGAACCCCA CCATCGTGGA GGTGCTGGAG GAATTCCCAT CTATCCAGAT GCCGGCCACC
    CTGCTCCTGA CCCAGCTGTC CCTGCTGCAG CCCCGCTACT ATTCCATCAG CTCCTCCCCA
    GACATGTACC CTGATGAAGT GCACCTCACT GTGGCCATCG TTTCCTACCG CACTCGAGAT
    GGAGAAGGAC CAATTCACCA CGGCGTATGC TCCTCCTGGC TCAACCGGAT ACAGGCTGAC
    GAACTGGTCC CCTGTTTCGT GAGAGGAGCA CCCAGCTTCC ACCTGCCCCG GAACCCCCAA
    GTCCCCTGCA TCCTCGTTGG ACCAGGCACC GGCATTGCCC CTTTCCGAAG CTTCTGGCAA
    CAGCGGCAAT TTGATATCCA ACACAAAGGA ATGAACCCCT GCCCCATGGT CCTGGTCTTC
    GGGTGCCGGC AATCCAAGAT AGATCATATC TACAGGGAAG AGACCCTGCA GGCCAAGAAC
    AAGGGGGTCT TCAGAGAGCT GTACACGGCT TACTCCCGGG AGCCAGACAA ACCAAAGAAG
    TATGTGCAGG ACATCCTGCA GGAGCAGCTG GCGGAGTCTG TGTACCGAGC CCTGAAGGAG
    CAAGGGGGCC ACATATACGT CTGTGGGGAC GTCACCATGG CTGCTGATGT CCTCAAAGCC
    ATCCAGCGCA TCATGACCCA GCAGGGGAAG CTCTCGGCAG AGGACGCCGG CGTATTCATC
    AGCCGGATGA GGGATGACAA CCGATACCAT GAGGATATTT TTGGAGTCAC CCTGCGAACG
    TACGAAGTGA CCAACCGCCT TAGATCTGAG TCCATTGCCT TCATTGAAGA GAGCAAAAAA
    GACACCGATG AGGTTTTCAG CTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016779820.1
    CDS2462..6868
    Translation

    Target ORF information:

    RefSeq Version XM_016924331.2
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes nitric oxide synthase 1 (NOS1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_016924331.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAGGATC ACATGTTCGG TGTTCAGCAA ATCCAGCCCA ATGTCATTTC TGTTCGTCTC 
    TTCAAGCGCA AAGTTGGGGG CCTGGGATTC CTGGTGAAGG AGCGGGTCAG TAAGCCGCCC
    GTGATTATCT CTGACCTGAT TCGTGGGGGC GCCGCAGAGC AGAGTGGCCT CATCCAGGCC
    GGAGACATCA TTCTTGCGGT CAACGGCCGG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC
    CTGGAGGTAC TCAGAGGCAT TGCCTCCGAG ACCCACGTGG TCCTCATTCT GAGGGGTCCT
    GAAGGTTTCA CCACGCACCT GGAGACCACC TTTACAGGTG ATGGGACCCC CAAGACCATC
    CGGGTGACAC AGCCCCTGGG TCCCCCCACC AAAGCCGTGG ATCTGTCCCA CCAGCCATCG
    GCCGGCAAAG AACAGCCGCT GGCAGTGGAT GGGGCCTCGG GTCCCGGGAA TGGGCCTCAG
    CATGCCTACG ATGACGGGCA GGAGGCTGGC TCACTCCCCC ATGCCAACGG CCTGGCCCCC
    AGGCCCCCAG GCCAGGACCC TGCGAAGAAA GCAACCAGAG TCAGCCTCCA AGGCAGAGGG
    GAGAACAATG AACTGCTCAA GGAGATAGAG CCTGTGCTGA GCCTTCTCAC CAGTGGGAGC
    AGAGGGGTCA AGGGAGGGGC ACCTGCCAAG GCAGAGATGA AAGATATGGG AATCCAGGTG
    GACAGAGATT TGGACGGCAA GTCACACAAA CCTCTGCCCC TCGCCGTGGA GAATGACCGA
    GTCTTCAATG ACCTATGGGG GAAGGGCAAT GTGCCTGTCG TCCTCAACAA CCCATATTCA
    GAGAAGGAGC AGCCCCCTGC CTCAGGAAAA CAGTCCCCCA CAAAGAATGG CAGCCCCTCC
    AAGTGTCCAC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG AGGTGGTTCT CACTGACACC
    CTCCACCTTA AGAGCACATT GGAAACGGGA TGCACTGAGT ACATCTGCAT GGGCTCCATC
    ATGCATCCTT CTCAGCATGC AAGGAGGCCT GAAGATGTCC GCACAAAAGA ACAGCTCTTC
    CCTCTCGCCA AAGAGTTTAT TGATCAATAC TATTCATCAA TTAAAAGATT TGGCTCCAAA
    GCCCACATGG AAAGGCTGGA AGAGGTGAAC AAAGAGATCG AAACCACTAG CACCTACCAG
    CTCAAGGACA CAGAGCTCAT CTATGGGGCC AAGCACGCCT GGCGGAATGC CTCGCGCTGT
    GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTATTCGATG CCCGTGACTG CACCACGGCC
    CACGGGATGT TCAACTACAT CTGTAACCAC GTCAAGTATG CCACCAACAA AGGGAACCTC
    AGGTCCGCCA TCACCATATT CCCCCAGAGG ACAGACGGCA AGCACGACTT CCGAGTCTGG
    AACTCCCAGC TCATCCGCTA CGCTGGCTAC AAGCAGCCCG ACGGCTCCAC CCTGGGGGAC
    CCAGCCAATG TGCAGTTCAC AGAGATATGC ATACAGCAGG GCTGGAAACC GCCTAGAGGC
    CGCTTCGATG TCCTGCCGCT CCTGCTTCAG GCCAACGGCA ATGACCCTGA GCTCTTCCAG
    ATTCCTCCAG AGCTGGTGTT GGAAGTTCCC ATCAGGCACC CCAAGTTTGA GTGGTTCAAG
    GACCTGGGGC TGAAGTGGTA CGGCCTCCCC GCCGTGTCCA ACATGCTCCT AGAGATTGGC
    GGCCTGGAGT TCAGCGCCTG TCCCTTCAGT GGCTGGTACA TGGGCACAGA GATTGGTGTC
    CGCGACTACT GTGACAACTC CCGCTACAAT ATCCTGGAGG AAGTGGCCAA GAAGATGAAC
    TTAGACATGA GGAAGATGTC CTCCCTGTGG AAGGACCAGG CGCTGGTGGA GATCAATATC
    GCGGTTCTCT ACAGCTTCCA GAGTGACAAA GTGACCATTG TTGACCATCA CTCCGCCACC
    GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGGGGCTG CCCTGCCGAC
    TGGGTGTGGA TCGTGCCCCC CATGTCCGGA AGCATCACCC CTGTGTTCCA CCAGGAGATG
    CTCAACTACC GGCTCACCCC CTCCTTCGAA TACCAGCCTG ATCCCTGGAA CACGCATGTC
    TGGAAAGGCA CCAACGGGAC CCCCACAAAG CGGCGAGCCA TCGGCTTCAA GAAGCTAGCA
    GAAGCTGTCA AGTTCTCGGC CAAGCTGATG GGGCAGGCTA TGGCCAAGAG GGTCAAAGCG
    ACCATCCTCT ATGCCACAGA GACAGGCAAA TCGCAAGCTT ATGCCAAGAC CTTGTGTGAG
    ATCTTCAAAC ACGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATCGTGCAC
    CTGGAACATG AAACTCTGGT CCTCGTGGTC ACCAGCACCT TTGGCAATGG AGATCCCCCT
    GAGAATGGGG AGAAATTCGG CTGTGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAG
    GAAGAAAGGA AGTACCCGGA ACCCTTGCGT TTCTTTCCCC GTAAAGGGCC TCCCCTCCCC
    AATGGTGACA CAGAAGTCCA CGGTCTGGCT GCAGCCCGTG ACAGCCAGCA CAGGAGCTAC
    AAGGTCCGAT TCAACAGCGT CTCCTCCTAC TCTGACTCCC AAAAATCATC AGGCGATGGG
    CCCGACCTCA GAGACAACTT TGAGAGTGCT GGACCCCTGG CCAATGTGAG GTTCTCAGTT
    TTTGGCCTCG GCTCACGAGC ATACCCTCAC TTTTGTGCCT TCGGACACGC TGTGGACACC
    CTCCTGGAAG AACTGGGAGG GGAGAGGATC CTGAAGATGA GGGAAGGGGA TGAGCTCTGT
    GGGCAGGAAG AGGCTTTCAG GACCTGGGCC AAGAAGGTCT TCAAGGCAGC CTGTGATGTC
    TTCTGTGTGG GAGATGATGT CAACATTGAA AAGGCCAACA ATTCCCTCAT CAGCAATGAT
    CGCAGCTGGA AGAGAAACAA GTTCCGCCTC ACCTTTGTGG CCGAAGCTCC AGAACTCACA
    CAAGGTCTAT CCAATGTCCA CAAAAAGCGA GTCTCAGCTG CCCGGCTCCT TAGCCGTCAA
    AACCTCCAGA GTCCTAAATC CAGTCGGTCA ACTATCTTCG TGCGTCTCCA CACCAACGGG
    AACCAGGAGC TGCAGTACCA GCCTGGGGAC CACCTGGGTG TCTTCCCTGG CAACCACGAG
    GACCTCGTGA ATGCCCTGAT CGAGCGGCTG GAGGACGCGC CCCCTGTCAA CCAGATGGTG
    AAAGTGGAAC TGCTGGAGGA GCGGAACACG GCTTTAGGTG TCATCAGTAA CTGGACAGAC
    GAGCTCCGCC TCCCGCCCTG CACCATCTTC CAGGCCTTCA AGTACTACCT GGACATCACC
    ACGCCACCAA CGCCCCTGCA GCTGCAGCAG TTTGCCTCCC TAGCTACCAG CGAGAAGGAG
    AAGCAGCGTC TGCTGGTCCT CAGCAAGGGT TTGCAGGAGT ACGAGGAATG GAAATGGGGC
    AAGAACCCCA CCATCGTGGA GGTGCTGGAG GAATTCCCAT CTATCCAGAT GCCGGCCACC
    CTGCTCCTGA CCCAGCTGTC CCTGCTGCAG CCCCGCTACT ATTCCATCAG CTCCTCCCCA
    GACATGTACC CTGATGAAGT GCACCTCACT GTGGCCATCG TTTCCTACCG CACTCGAGAT
    GGAGAAGGAC CAATTCACCA CGGCGTATGC TCCTCCTGGC TCAACCGGAT ACAGGCTGAC
    GAACTGGTCC CCTGTTTCGT GAGAGGAGCA CCCAGCTTCC ACCTGCCCCG GAACCCCCAA
    GTCCCCTGCA TCCTCGTTGG ACCAGGCACC GGCATTGCCC CTTTCCGAAG CTTCTGGCAA
    CAGCGGCAAT TTGATATCCA ACACAAAGGA ATGAACCCCT GCCCCATGGT CCTGGTCTTC
    GGGTGCCGGC AATCCAAGAT AGATCATATC TACAGGGAAG AGACCCTGCA GGCCAAGAAC
    AAGGGGGTCT TCAGAGAGCT GTACACGGCT TACTCCCGGG AGCCAGACAA ACCAAAGAAG
    TATGTGCAGG ACATCCTGCA GGAGCAGCTG GCGGAGTCTG TGTACCGAGC CCTGAAGGAG
    CAAGGGGGCC ACATATACGT CTGTGGGGAC GTCACCATGG CTGCTGATGT CCTCAAAGCC
    ATCCAGCGCA TCATGACCCA GCAGGGGAAG CTCTCGGCAG AGGACGCCGG CGTATTCATC
    AGCCGGATGA GGGATGACAA CCGATACCAT GAGGATATTT TTGGAGTCAC CCTGCGAACG
    TACGAAGTGA CCAACCGCCT TAGATCTGAG TCCATTGCCT TCATTGAAGA GAGCAAAAAA
    GACACCGATG AGGTTTTCAG CTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc66615
    Clone ID Related Accession (Same CDS sequence) XM_016924331.1 , XM_016924327.2 , XM_016924331.2 , XM_016924328.1 , XM_016924332.2 , XM_016924327.1 , XM_016924332.1 , XM_016924328.2 , XM_016924329.2 , XM_016924329.1
    Accession Version XM_016924328.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4407bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-01
    Organism Pan troglodytes(chimpanzee)
    Product nitric oxide synthase, brain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006479.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAGGATC ACATGTTCGG TGTTCAGCAA ATCCAGCCCA ATGTCATTTC TGTTCGTCTC 
    TTCAAGCGCA AAGTTGGGGG CCTGGGATTC CTGGTGAAGG AGCGGGTCAG TAAGCCGCCC
    GTGATTATCT CTGACCTGAT TCGTGGGGGC GCCGCAGAGC AGAGTGGCCT CATCCAGGCC
    GGAGACATCA TTCTTGCGGT CAACGGCCGG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC
    CTGGAGGTAC TCAGAGGCAT TGCCTCCGAG ACCCACGTGG TCCTCATTCT GAGGGGTCCT
    GAAGGTTTCA CCACGCACCT GGAGACCACC TTTACAGGTG ATGGGACCCC CAAGACCATC
    CGGGTGACAC AGCCCCTGGG TCCCCCCACC AAAGCCGTGG ATCTGTCCCA CCAGCCATCG
    GCCGGCAAAG AACAGCCGCT GGCAGTGGAT GGGGCCTCGG GTCCCGGGAA TGGGCCTCAG
    CATGCCTACG ATGACGGGCA GGAGGCTGGC TCACTCCCCC ATGCCAACGG CCTGGCCCCC
    AGGCCCCCAG GCCAGGACCC TGCGAAGAAA GCAACCAGAG TCAGCCTCCA AGGCAGAGGG
    GAGAACAATG AACTGCTCAA GGAGATAGAG CCTGTGCTGA GCCTTCTCAC CAGTGGGAGC
    AGAGGGGTCA AGGGAGGGGC ACCTGCCAAG GCAGAGATGA AAGATATGGG AATCCAGGTG
    GACAGAGATT TGGACGGCAA GTCACACAAA CCTCTGCCCC TCGCCGTGGA GAATGACCGA
    GTCTTCAATG ACCTATGGGG GAAGGGCAAT GTGCCTGTCG TCCTCAACAA CCCATATTCA
    GAGAAGGAGC AGCCCCCTGC CTCAGGAAAA CAGTCCCCCA CAAAGAATGG CAGCCCCTCC
    AAGTGTCCAC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG AGGTGGTTCT CACTGACACC
    CTCCACCTTA AGAGCACATT GGAAACGGGA TGCACTGAGT ACATCTGCAT GGGCTCCATC
    ATGCATCCTT CTCAGCATGC AAGGAGGCCT GAAGATGTCC GCACAAAAGA ACAGCTCTTC
    CCTCTCGCCA AAGAGTTTAT TGATCAATAC TATTCATCAA TTAAAAGATT TGGCTCCAAA
    GCCCACATGG AAAGGCTGGA AGAGGTGAAC AAAGAGATCG AAACCACTAG CACCTACCAG
    CTCAAGGACA CAGAGCTCAT CTATGGGGCC AAGCACGCCT GGCGGAATGC CTCGCGCTGT
    GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTATTCGATG CCCGTGACTG CACCACGGCC
    CACGGGATGT TCAACTACAT CTGTAACCAC GTCAAGTATG CCACCAACAA AGGGAACCTC
    AGGTCCGCCA TCACCATATT CCCCCAGAGG ACAGACGGCA AGCACGACTT CCGAGTCTGG
    AACTCCCAGC TCATCCGCTA CGCTGGCTAC AAGCAGCCCG ACGGCTCCAC CCTGGGGGAC
    CCAGCCAATG TGCAGTTCAC AGAGATATGC ATACAGCAGG GCTGGAAACC GCCTAGAGGC
    CGCTTCGATG TCCTGCCGCT CCTGCTTCAG GCCAACGGCA ATGACCCTGA GCTCTTCCAG
    ATTCCTCCAG AGCTGGTGTT GGAAGTTCCC ATCAGGCACC CCAAGTTTGA GTGGTTCAAG
    GACCTGGGGC TGAAGTGGTA CGGCCTCCCC GCCGTGTCCA ACATGCTCCT AGAGATTGGC
    GGCCTGGAGT TCAGCGCCTG TCCCTTCAGT GGCTGGTACA TGGGCACAGA GATTGGTGTC
    CGCGACTACT GTGACAACTC CCGCTACAAT ATCCTGGAGG AAGTGGCCAA GAAGATGAAC
    TTAGACATGA GGAAGATGTC CTCCCTGTGG AAGGACCAGG CGCTGGTGGA GATCAATATC
    GCGGTTCTCT ACAGCTTCCA GAGTGACAAA GTGACCATTG TTGACCATCA CTCCGCCACC
    GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGGGGCTG CCCTGCCGAC
    TGGGTGTGGA TCGTGCCCCC CATGTCCGGA AGCATCACCC CTGTGTTCCA CCAGGAGATG
    CTCAACTACC GGCTCACCCC CTCCTTCGAA TACCAGCCTG ATCCCTGGAA CACGCATGTC
    TGGAAAGGCA CCAACGGGAC CCCCACAAAG CGGCGAGCCA TCGGCTTCAA GAAGCTAGCA
    GAAGCTGTCA AGTTCTCGGC CAAGCTGATG GGGCAGGCTA TGGCCAAGAG GGTCAAAGCG
    ACCATCCTCT ATGCCACAGA GACAGGCAAA TCGCAAGCTT ATGCCAAGAC CTTGTGTGAG
    ATCTTCAAAC ACGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATCGTGCAC
    CTGGAACATG AAACTCTGGT CCTCGTGGTC ACCAGCACCT TTGGCAATGG AGATCCCCCT
    GAGAATGGGG AGAAATTCGG CTGTGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAG
    GAAGAAAGGA AGTACCCGGA ACCCTTGCGT TTCTTTCCCC GTAAAGGGCC TCCCCTCCCC
    AATGGTGACA CAGAAGTCCA CGGTCTGGCT GCAGCCCGTG ACAGCCAGCA CAGGAGCTAC
    AAGGTCCGAT TCAACAGCGT CTCCTCCTAC TCTGACTCCC AAAAATCATC AGGCGATGGG
    CCCGACCTCA GAGACAACTT TGAGAGTGCT GGACCCCTGG CCAATGTGAG GTTCTCAGTT
    TTTGGCCTCG GCTCACGAGC ATACCCTCAC TTTTGTGCCT TCGGACACGC TGTGGACACC
    CTCCTGGAAG AACTGGGAGG GGAGAGGATC CTGAAGATGA GGGAAGGGGA TGAGCTCTGT
    GGGCAGGAAG AGGCTTTCAG GACCTGGGCC AAGAAGGTCT TCAAGGCAGC CTGTGATGTC
    TTCTGTGTGG GAGATGATGT CAACATTGAA AAGGCCAACA ATTCCCTCAT CAGCAATGAT
    CGCAGCTGGA AGAGAAACAA GTTCCGCCTC ACCTTTGTGG CCGAAGCTCC AGAACTCACA
    CAAGGTCTAT CCAATGTCCA CAAAAAGCGA GTCTCAGCTG CCCGGCTCCT TAGCCGTCAA
    AACCTCCAGA GTCCTAAATC CAGTCGGTCA ACTATCTTCG TGCGTCTCCA CACCAACGGG
    AACCAGGAGC TGCAGTACCA GCCTGGGGAC CACCTGGGTG TCTTCCCTGG CAACCACGAG
    GACCTCGTGA ATGCCCTGAT CGAGCGGCTG GAGGACGCGC CCCCTGTCAA CCAGATGGTG
    AAAGTGGAAC TGCTGGAGGA GCGGAACACG GCTTTAGGTG TCATCAGTAA CTGGACAGAC
    GAGCTCCGCC TCCCGCCCTG CACCATCTTC CAGGCCTTCA AGTACTACCT GGACATCACC
    ACGCCACCAA CGCCCCTGCA GCTGCAGCAG TTTGCCTCCC TAGCTACCAG CGAGAAGGAG
    AAGCAGCGTC TGCTGGTCCT CAGCAAGGGT TTGCAGGAGT ACGAGGAATG GAAATGGGGC
    AAGAACCCCA CCATCGTGGA GGTGCTGGAG GAATTCCCAT CTATCCAGAT GCCGGCCACC
    CTGCTCCTGA CCCAGCTGTC CCTGCTGCAG CCCCGCTACT ATTCCATCAG CTCCTCCCCA
    GACATGTACC CTGATGAAGT GCACCTCACT GTGGCCATCG TTTCCTACCG CACTCGAGAT
    GGAGAAGGAC CAATTCACCA CGGCGTATGC TCCTCCTGGC TCAACCGGAT ACAGGCTGAC
    GAACTGGTCC CCTGTTTCGT GAGAGGAGCA CCCAGCTTCC ACCTGCCCCG GAACCCCCAA
    GTCCCCTGCA TCCTCGTTGG ACCAGGCACC GGCATTGCCC CTTTCCGAAG CTTCTGGCAA
    CAGCGGCAAT TTGATATCCA ACACAAAGGA ATGAACCCCT GCCCCATGGT CCTGGTCTTC
    GGGTGCCGGC AATCCAAGAT AGATCATATC TACAGGGAAG AGACCCTGCA GGCCAAGAAC
    AAGGGGGTCT TCAGAGAGCT GTACACGGCT TACTCCCGGG AGCCAGACAA ACCAAAGAAG
    TATGTGCAGG ACATCCTGCA GGAGCAGCTG GCGGAGTCTG TGTACCGAGC CCTGAAGGAG
    CAAGGGGGCC ACATATACGT CTGTGGGGAC GTCACCATGG CTGCTGATGT CCTCAAAGCC
    ATCCAGCGCA TCATGACCCA GCAGGGGAAG CTCTCGGCAG AGGACGCCGG CGTATTCATC
    AGCCGGATGA GGGATGACAA CCGATACCAT GAGGATATTT TTGGAGTCAC CCTGCGAACG
    TACGAAGTGA CCAACCGCCT TAGATCTGAG TCCATTGCCT TCATTGAAGA GAGCAAAAAA
    GACACCGATG AGGTTTTCAG CTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016779817.1
    CDS712..5118
    Translation

    Target ORF information:

    RefSeq Version XM_016924328.1
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes nitric oxide synthase 1 (NOS1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_016924328.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAGGATC ACATGTTCGG TGTTCAGCAA ATCCAGCCCA ATGTCATTTC TGTTCGTCTC 
    TTCAAGCGCA AAGTTGGGGG CCTGGGATTC CTGGTGAAGG AGCGGGTCAG TAAGCCGCCC
    GTGATTATCT CTGACCTGAT TCGTGGGGGC GCCGCAGAGC AGAGTGGCCT CATCCAGGCC
    GGAGACATCA TTCTTGCGGT CAACGGCCGG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC
    CTGGAGGTAC TCAGAGGCAT TGCCTCCGAG ACCCACGTGG TCCTCATTCT GAGGGGTCCT
    GAAGGTTTCA CCACGCACCT GGAGACCACC TTTACAGGTG ATGGGACCCC CAAGACCATC
    CGGGTGACAC AGCCCCTGGG TCCCCCCACC AAAGCCGTGG ATCTGTCCCA CCAGCCATCG
    GCCGGCAAAG AACAGCCGCT GGCAGTGGAT GGGGCCTCGG GTCCCGGGAA TGGGCCTCAG
    CATGCCTACG ATGACGGGCA GGAGGCTGGC TCACTCCCCC ATGCCAACGG CCTGGCCCCC
    AGGCCCCCAG GCCAGGACCC TGCGAAGAAA GCAACCAGAG TCAGCCTCCA AGGCAGAGGG
    GAGAACAATG AACTGCTCAA GGAGATAGAG CCTGTGCTGA GCCTTCTCAC CAGTGGGAGC
    AGAGGGGTCA AGGGAGGGGC ACCTGCCAAG GCAGAGATGA AAGATATGGG AATCCAGGTG
    GACAGAGATT TGGACGGCAA GTCACACAAA CCTCTGCCCC TCGCCGTGGA GAATGACCGA
    GTCTTCAATG ACCTATGGGG GAAGGGCAAT GTGCCTGTCG TCCTCAACAA CCCATATTCA
    GAGAAGGAGC AGCCCCCTGC CTCAGGAAAA CAGTCCCCCA CAAAGAATGG CAGCCCCTCC
    AAGTGTCCAC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG AGGTGGTTCT CACTGACACC
    CTCCACCTTA AGAGCACATT GGAAACGGGA TGCACTGAGT ACATCTGCAT GGGCTCCATC
    ATGCATCCTT CTCAGCATGC AAGGAGGCCT GAAGATGTCC GCACAAAAGA ACAGCTCTTC
    CCTCTCGCCA AAGAGTTTAT TGATCAATAC TATTCATCAA TTAAAAGATT TGGCTCCAAA
    GCCCACATGG AAAGGCTGGA AGAGGTGAAC AAAGAGATCG AAACCACTAG CACCTACCAG
    CTCAAGGACA CAGAGCTCAT CTATGGGGCC AAGCACGCCT GGCGGAATGC CTCGCGCTGT
    GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTATTCGATG CCCGTGACTG CACCACGGCC
    CACGGGATGT TCAACTACAT CTGTAACCAC GTCAAGTATG CCACCAACAA AGGGAACCTC
    AGGTCCGCCA TCACCATATT CCCCCAGAGG ACAGACGGCA AGCACGACTT CCGAGTCTGG
    AACTCCCAGC TCATCCGCTA CGCTGGCTAC AAGCAGCCCG ACGGCTCCAC CCTGGGGGAC
    CCAGCCAATG TGCAGTTCAC AGAGATATGC ATACAGCAGG GCTGGAAACC GCCTAGAGGC
    CGCTTCGATG TCCTGCCGCT CCTGCTTCAG GCCAACGGCA ATGACCCTGA GCTCTTCCAG
    ATTCCTCCAG AGCTGGTGTT GGAAGTTCCC ATCAGGCACC CCAAGTTTGA GTGGTTCAAG
    GACCTGGGGC TGAAGTGGTA CGGCCTCCCC GCCGTGTCCA ACATGCTCCT AGAGATTGGC
    GGCCTGGAGT TCAGCGCCTG TCCCTTCAGT GGCTGGTACA TGGGCACAGA GATTGGTGTC
    CGCGACTACT GTGACAACTC CCGCTACAAT ATCCTGGAGG AAGTGGCCAA GAAGATGAAC
    TTAGACATGA GGAAGATGTC CTCCCTGTGG AAGGACCAGG CGCTGGTGGA GATCAATATC
    GCGGTTCTCT ACAGCTTCCA GAGTGACAAA GTGACCATTG TTGACCATCA CTCCGCCACC
    GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGGGGCTG CCCTGCCGAC
    TGGGTGTGGA TCGTGCCCCC CATGTCCGGA AGCATCACCC CTGTGTTCCA CCAGGAGATG
    CTCAACTACC GGCTCACCCC CTCCTTCGAA TACCAGCCTG ATCCCTGGAA CACGCATGTC
    TGGAAAGGCA CCAACGGGAC CCCCACAAAG CGGCGAGCCA TCGGCTTCAA GAAGCTAGCA
    GAAGCTGTCA AGTTCTCGGC CAAGCTGATG GGGCAGGCTA TGGCCAAGAG GGTCAAAGCG
    ACCATCCTCT ATGCCACAGA GACAGGCAAA TCGCAAGCTT ATGCCAAGAC CTTGTGTGAG
    ATCTTCAAAC ACGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATCGTGCAC
    CTGGAACATG AAACTCTGGT CCTCGTGGTC ACCAGCACCT TTGGCAATGG AGATCCCCCT
    GAGAATGGGG AGAAATTCGG CTGTGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAG
    GAAGAAAGGA AGTACCCGGA ACCCTTGCGT TTCTTTCCCC GTAAAGGGCC TCCCCTCCCC
    AATGGTGACA CAGAAGTCCA CGGTCTGGCT GCAGCCCGTG ACAGCCAGCA CAGGAGCTAC
    AAGGTCCGAT TCAACAGCGT CTCCTCCTAC TCTGACTCCC AAAAATCATC AGGCGATGGG
    CCCGACCTCA GAGACAACTT TGAGAGTGCT GGACCCCTGG CCAATGTGAG GTTCTCAGTT
    TTTGGCCTCG GCTCACGAGC ATACCCTCAC TTTTGTGCCT TCGGACACGC TGTGGACACC
    CTCCTGGAAG AACTGGGAGG GGAGAGGATC CTGAAGATGA GGGAAGGGGA TGAGCTCTGT
    GGGCAGGAAG AGGCTTTCAG GACCTGGGCC AAGAAGGTCT TCAAGGCAGC CTGTGATGTC
    TTCTGTGTGG GAGATGATGT CAACATTGAA AAGGCCAACA ATTCCCTCAT CAGCAATGAT
    CGCAGCTGGA AGAGAAACAA GTTCCGCCTC ACCTTTGTGG CCGAAGCTCC AGAACTCACA
    CAAGGTCTAT CCAATGTCCA CAAAAAGCGA GTCTCAGCTG CCCGGCTCCT TAGCCGTCAA
    AACCTCCAGA GTCCTAAATC CAGTCGGTCA ACTATCTTCG TGCGTCTCCA CACCAACGGG
    AACCAGGAGC TGCAGTACCA GCCTGGGGAC CACCTGGGTG TCTTCCCTGG CAACCACGAG
    GACCTCGTGA ATGCCCTGAT CGAGCGGCTG GAGGACGCGC CCCCTGTCAA CCAGATGGTG
    AAAGTGGAAC TGCTGGAGGA GCGGAACACG GCTTTAGGTG TCATCAGTAA CTGGACAGAC
    GAGCTCCGCC TCCCGCCCTG CACCATCTTC CAGGCCTTCA AGTACTACCT GGACATCACC
    ACGCCACCAA CGCCCCTGCA GCTGCAGCAG TTTGCCTCCC TAGCTACCAG CGAGAAGGAG
    AAGCAGCGTC TGCTGGTCCT CAGCAAGGGT TTGCAGGAGT ACGAGGAATG GAAATGGGGC
    AAGAACCCCA CCATCGTGGA GGTGCTGGAG GAATTCCCAT CTATCCAGAT GCCGGCCACC
    CTGCTCCTGA CCCAGCTGTC CCTGCTGCAG CCCCGCTACT ATTCCATCAG CTCCTCCCCA
    GACATGTACC CTGATGAAGT GCACCTCACT GTGGCCATCG TTTCCTACCG CACTCGAGAT
    GGAGAAGGAC CAATTCACCA CGGCGTATGC TCCTCCTGGC TCAACCGGAT ACAGGCTGAC
    GAACTGGTCC CCTGTTTCGT GAGAGGAGCA CCCAGCTTCC ACCTGCCCCG GAACCCCCAA
    GTCCCCTGCA TCCTCGTTGG ACCAGGCACC GGCATTGCCC CTTTCCGAAG CTTCTGGCAA
    CAGCGGCAAT TTGATATCCA ACACAAAGGA ATGAACCCCT GCCCCATGGT CCTGGTCTTC
    GGGTGCCGGC AATCCAAGAT AGATCATATC TACAGGGAAG AGACCCTGCA GGCCAAGAAC
    AAGGGGGTCT TCAGAGAGCT GTACACGGCT TACTCCCGGG AGCCAGACAA ACCAAAGAAG
    TATGTGCAGG ACATCCTGCA GGAGCAGCTG GCGGAGTCTG TGTACCGAGC CCTGAAGGAG
    CAAGGGGGCC ACATATACGT CTGTGGGGAC GTCACCATGG CTGCTGATGT CCTCAAAGCC
    ATCCAGCGCA TCATGACCCA GCAGGGGAAG CTCTCGGCAG AGGACGCCGG CGTATTCATC
    AGCCGGATGA GGGATGACAA CCGATACCAT GAGGATATTT TTGGAGTCAC CCTGCGAACG
    TACGAAGTGA CCAACCGCCT TAGATCTGAG TCCATTGCCT TCATTGAAGA GAGCAAAAAA
    GACACCGATG AGGTTTTCAG CTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc66615
    Clone ID Related Accession (Same CDS sequence) XM_016924331.1 , XM_016924327.2 , XM_016924331.2 , XM_016924328.1 , XM_016924332.2 , XM_016924327.1 , XM_016924332.1 , XM_016924328.2 , XM_016924329.2 , XM_016924329.1
    Accession Version XM_016924332.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4407bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-19
    Organism Pan troglodytes(chimpanzee)
    Product nitric oxide synthase, brain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036891.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016924332.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAGGATC ACATGTTCGG TGTTCAGCAA ATCCAGCCCA ATGTCATTTC TGTTCGTCTC 
    TTCAAGCGCA AAGTTGGGGG CCTGGGATTC CTGGTGAAGG AGCGGGTCAG TAAGCCGCCC
    GTGATTATCT CTGACCTGAT TCGTGGGGGC GCCGCAGAGC AGAGTGGCCT CATCCAGGCC
    GGAGACATCA TTCTTGCGGT CAACGGCCGG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC
    CTGGAGGTAC TCAGAGGCAT TGCCTCCGAG ACCCACGTGG TCCTCATTCT GAGGGGTCCT
    GAAGGTTTCA CCACGCACCT GGAGACCACC TTTACAGGTG ATGGGACCCC CAAGACCATC
    CGGGTGACAC AGCCCCTGGG TCCCCCCACC AAAGCCGTGG ATCTGTCCCA CCAGCCATCG
    GCCGGCAAAG AACAGCCGCT GGCAGTGGAT GGGGCCTCGG GTCCCGGGAA TGGGCCTCAG
    CATGCCTACG ATGACGGGCA GGAGGCTGGC TCACTCCCCC ATGCCAACGG CCTGGCCCCC
    AGGCCCCCAG GCCAGGACCC TGCGAAGAAA GCAACCAGAG TCAGCCTCCA AGGCAGAGGG
    GAGAACAATG AACTGCTCAA GGAGATAGAG CCTGTGCTGA GCCTTCTCAC CAGTGGGAGC
    AGAGGGGTCA AGGGAGGGGC ACCTGCCAAG GCAGAGATGA AAGATATGGG AATCCAGGTG
    GACAGAGATT TGGACGGCAA GTCACACAAA CCTCTGCCCC TCGCCGTGGA GAATGACCGA
    GTCTTCAATG ACCTATGGGG GAAGGGCAAT GTGCCTGTCG TCCTCAACAA CCCATATTCA
    GAGAAGGAGC AGCCCCCTGC CTCAGGAAAA CAGTCCCCCA CAAAGAATGG CAGCCCCTCC
    AAGTGTCCAC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG AGGTGGTTCT CACTGACACC
    CTCCACCTTA AGAGCACATT GGAAACGGGA TGCACTGAGT ACATCTGCAT GGGCTCCATC
    ATGCATCCTT CTCAGCATGC AAGGAGGCCT GAAGATGTCC GCACAAAAGA ACAGCTCTTC
    CCTCTCGCCA AAGAGTTTAT TGATCAATAC TATTCATCAA TTAAAAGATT TGGCTCCAAA
    GCCCACATGG AAAGGCTGGA AGAGGTGAAC AAAGAGATCG AAACCACTAG CACCTACCAG
    CTCAAGGACA CAGAGCTCAT CTATGGGGCC AAGCACGCCT GGCGGAATGC CTCGCGCTGT
    GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTATTCGATG CCCGTGACTG CACCACGGCC
    CACGGGATGT TCAACTACAT CTGTAACCAC GTCAAGTATG CCACCAACAA AGGGAACCTC
    AGGTCCGCCA TCACCATATT CCCCCAGAGG ACAGACGGCA AGCACGACTT CCGAGTCTGG
    AACTCCCAGC TCATCCGCTA CGCTGGCTAC AAGCAGCCCG ACGGCTCCAC CCTGGGGGAC
    CCAGCCAATG TGCAGTTCAC AGAGATATGC ATACAGCAGG GCTGGAAACC GCCTAGAGGC
    CGCTTCGATG TCCTGCCGCT CCTGCTTCAG GCCAACGGCA ATGACCCTGA GCTCTTCCAG
    ATTCCTCCAG AGCTGGTGTT GGAAGTTCCC ATCAGGCACC CCAAGTTTGA GTGGTTCAAG
    GACCTGGGGC TGAAGTGGTA CGGCCTCCCC GCCGTGTCCA ACATGCTCCT AGAGATTGGC
    GGCCTGGAGT TCAGCGCCTG TCCCTTCAGT GGCTGGTACA TGGGCACAGA GATTGGTGTC
    CGCGACTACT GTGACAACTC CCGCTACAAT ATCCTGGAGG AAGTGGCCAA GAAGATGAAC
    TTAGACATGA GGAAGATGTC CTCCCTGTGG AAGGACCAGG CGCTGGTGGA GATCAATATC
    GCGGTTCTCT ACAGCTTCCA GAGTGACAAA GTGACCATTG TTGACCATCA CTCCGCCACC
    GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGGGGCTG CCCTGCCGAC
    TGGGTGTGGA TCGTGCCCCC CATGTCCGGA AGCATCACCC CTGTGTTCCA CCAGGAGATG
    CTCAACTACC GGCTCACCCC CTCCTTCGAA TACCAGCCTG ATCCCTGGAA CACGCATGTC
    TGGAAAGGCA CCAACGGGAC CCCCACAAAG CGGCGAGCCA TCGGCTTCAA GAAGCTAGCA
    GAAGCTGTCA AGTTCTCGGC CAAGCTGATG GGGCAGGCTA TGGCCAAGAG GGTCAAAGCG
    ACCATCCTCT ATGCCACAGA GACAGGCAAA TCGCAAGCTT ATGCCAAGAC CTTGTGTGAG
    ATCTTCAAAC ACGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATCGTGCAC
    CTGGAACATG AAACTCTGGT CCTCGTGGTC ACCAGCACCT TTGGCAATGG AGATCCCCCT
    GAGAATGGGG AGAAATTCGG CTGTGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAG
    GAAGAAAGGA AGTACCCGGA ACCCTTGCGT TTCTTTCCCC GTAAAGGGCC TCCCCTCCCC
    AATGGTGACA CAGAAGTCCA CGGTCTGGCT GCAGCCCGTG ACAGCCAGCA CAGGAGCTAC
    AAGGTCCGAT TCAACAGCGT CTCCTCCTAC TCTGACTCCC AAAAATCATC AGGCGATGGG
    CCCGACCTCA GAGACAACTT TGAGAGTGCT GGACCCCTGG CCAATGTGAG GTTCTCAGTT
    TTTGGCCTCG GCTCACGAGC ATACCCTCAC TTTTGTGCCT TCGGACACGC TGTGGACACC
    CTCCTGGAAG AACTGGGAGG GGAGAGGATC CTGAAGATGA GGGAAGGGGA TGAGCTCTGT
    GGGCAGGAAG AGGCTTTCAG GACCTGGGCC AAGAAGGTCT TCAAGGCAGC CTGTGATGTC
    TTCTGTGTGG GAGATGATGT CAACATTGAA AAGGCCAACA ATTCCCTCAT CAGCAATGAT
    CGCAGCTGGA AGAGAAACAA GTTCCGCCTC ACCTTTGTGG CCGAAGCTCC AGAACTCACA
    CAAGGTCTAT CCAATGTCCA CAAAAAGCGA GTCTCAGCTG CCCGGCTCCT TAGCCGTCAA
    AACCTCCAGA GTCCTAAATC CAGTCGGTCA ACTATCTTCG TGCGTCTCCA CACCAACGGG
    AACCAGGAGC TGCAGTACCA GCCTGGGGAC CACCTGGGTG TCTTCCCTGG CAACCACGAG
    GACCTCGTGA ATGCCCTGAT CGAGCGGCTG GAGGACGCGC CCCCTGTCAA CCAGATGGTG
    AAAGTGGAAC TGCTGGAGGA GCGGAACACG GCTTTAGGTG TCATCAGTAA CTGGACAGAC
    GAGCTCCGCC TCCCGCCCTG CACCATCTTC CAGGCCTTCA AGTACTACCT GGACATCACC
    ACGCCACCAA CGCCCCTGCA GCTGCAGCAG TTTGCCTCCC TAGCTACCAG CGAGAAGGAG
    AAGCAGCGTC TGCTGGTCCT CAGCAAGGGT TTGCAGGAGT ACGAGGAATG GAAATGGGGC
    AAGAACCCCA CCATCGTGGA GGTGCTGGAG GAATTCCCAT CTATCCAGAT GCCGGCCACC
    CTGCTCCTGA CCCAGCTGTC CCTGCTGCAG CCCCGCTACT ATTCCATCAG CTCCTCCCCA
    GACATGTACC CTGATGAAGT GCACCTCACT GTGGCCATCG TTTCCTACCG CACTCGAGAT
    GGAGAAGGAC CAATTCACCA CGGCGTATGC TCCTCCTGGC TCAACCGGAT ACAGGCTGAC
    GAACTGGTCC CCTGTTTCGT GAGAGGAGCA CCCAGCTTCC ACCTGCCCCG GAACCCCCAA
    GTCCCCTGCA TCCTCGTTGG ACCAGGCACC GGCATTGCCC CTTTCCGAAG CTTCTGGCAA
    CAGCGGCAAT TTGATATCCA ACACAAAGGA ATGAACCCCT GCCCCATGGT CCTGGTCTTC
    GGGTGCCGGC AATCCAAGAT AGATCATATC TACAGGGAAG AGACCCTGCA GGCCAAGAAC
    AAGGGGGTCT TCAGAGAGCT GTACACGGCT TACTCCCGGG AGCCAGACAA ACCAAAGAAG
    TATGTGCAGG ACATCCTGCA GGAGCAGCTG GCGGAGTCTG TGTACCGAGC CCTGAAGGAG
    CAAGGGGGCC ACATATACGT CTGTGGGGAC GTCACCATGG CTGCTGATGT CCTCAAAGCC
    ATCCAGCGCA TCATGACCCA GCAGGGGAAG CTCTCGGCAG AGGACGCCGG CGTATTCATC
    AGCCGGATGA GGGATGACAA CCGATACCAT GAGGATATTT TTGGAGTCAC CCTGCGAACG
    TACGAAGTGA CCAACCGCCT TAGATCTGAG TCCATTGCCT TCATTGAAGA GAGCAAAAAA
    GACACCGATG AGGTTTTCAG CTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016779821.1
    CDS506..4912
    Translation

    Target ORF information:

    RefSeq Version XM_016924332.2
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes nitric oxide synthase 1 (NOS1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_016924332.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAGGATC ACATGTTCGG TGTTCAGCAA ATCCAGCCCA ATGTCATTTC TGTTCGTCTC 
    TTCAAGCGCA AAGTTGGGGG CCTGGGATTC CTGGTGAAGG AGCGGGTCAG TAAGCCGCCC
    GTGATTATCT CTGACCTGAT TCGTGGGGGC GCCGCAGAGC AGAGTGGCCT CATCCAGGCC
    GGAGACATCA TTCTTGCGGT CAACGGCCGG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC
    CTGGAGGTAC TCAGAGGCAT TGCCTCCGAG ACCCACGTGG TCCTCATTCT GAGGGGTCCT
    GAAGGTTTCA CCACGCACCT GGAGACCACC TTTACAGGTG ATGGGACCCC CAAGACCATC
    CGGGTGACAC AGCCCCTGGG TCCCCCCACC AAAGCCGTGG ATCTGTCCCA CCAGCCATCG
    GCCGGCAAAG AACAGCCGCT GGCAGTGGAT GGGGCCTCGG GTCCCGGGAA TGGGCCTCAG
    CATGCCTACG ATGACGGGCA GGAGGCTGGC TCACTCCCCC ATGCCAACGG CCTGGCCCCC
    AGGCCCCCAG GCCAGGACCC TGCGAAGAAA GCAACCAGAG TCAGCCTCCA AGGCAGAGGG
    GAGAACAATG AACTGCTCAA GGAGATAGAG CCTGTGCTGA GCCTTCTCAC CAGTGGGAGC
    AGAGGGGTCA AGGGAGGGGC ACCTGCCAAG GCAGAGATGA AAGATATGGG AATCCAGGTG
    GACAGAGATT TGGACGGCAA GTCACACAAA CCTCTGCCCC TCGCCGTGGA GAATGACCGA
    GTCTTCAATG ACCTATGGGG GAAGGGCAAT GTGCCTGTCG TCCTCAACAA CCCATATTCA
    GAGAAGGAGC AGCCCCCTGC CTCAGGAAAA CAGTCCCCCA CAAAGAATGG CAGCCCCTCC
    AAGTGTCCAC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG AGGTGGTTCT CACTGACACC
    CTCCACCTTA AGAGCACATT GGAAACGGGA TGCACTGAGT ACATCTGCAT GGGCTCCATC
    ATGCATCCTT CTCAGCATGC AAGGAGGCCT GAAGATGTCC GCACAAAAGA ACAGCTCTTC
    CCTCTCGCCA AAGAGTTTAT TGATCAATAC TATTCATCAA TTAAAAGATT TGGCTCCAAA
    GCCCACATGG AAAGGCTGGA AGAGGTGAAC AAAGAGATCG AAACCACTAG CACCTACCAG
    CTCAAGGACA CAGAGCTCAT CTATGGGGCC AAGCACGCCT GGCGGAATGC CTCGCGCTGT
    GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTATTCGATG CCCGTGACTG CACCACGGCC
    CACGGGATGT TCAACTACAT CTGTAACCAC GTCAAGTATG CCACCAACAA AGGGAACCTC
    AGGTCCGCCA TCACCATATT CCCCCAGAGG ACAGACGGCA AGCACGACTT CCGAGTCTGG
    AACTCCCAGC TCATCCGCTA CGCTGGCTAC AAGCAGCCCG ACGGCTCCAC CCTGGGGGAC
    CCAGCCAATG TGCAGTTCAC AGAGATATGC ATACAGCAGG GCTGGAAACC GCCTAGAGGC
    CGCTTCGATG TCCTGCCGCT CCTGCTTCAG GCCAACGGCA ATGACCCTGA GCTCTTCCAG
    ATTCCTCCAG AGCTGGTGTT GGAAGTTCCC ATCAGGCACC CCAAGTTTGA GTGGTTCAAG
    GACCTGGGGC TGAAGTGGTA CGGCCTCCCC GCCGTGTCCA ACATGCTCCT AGAGATTGGC
    GGCCTGGAGT TCAGCGCCTG TCCCTTCAGT GGCTGGTACA TGGGCACAGA GATTGGTGTC
    CGCGACTACT GTGACAACTC CCGCTACAAT ATCCTGGAGG AAGTGGCCAA GAAGATGAAC
    TTAGACATGA GGAAGATGTC CTCCCTGTGG AAGGACCAGG CGCTGGTGGA GATCAATATC
    GCGGTTCTCT ACAGCTTCCA GAGTGACAAA GTGACCATTG TTGACCATCA CTCCGCCACC
    GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGGGGCTG CCCTGCCGAC
    TGGGTGTGGA TCGTGCCCCC CATGTCCGGA AGCATCACCC CTGTGTTCCA CCAGGAGATG
    CTCAACTACC GGCTCACCCC CTCCTTCGAA TACCAGCCTG ATCCCTGGAA CACGCATGTC
    TGGAAAGGCA CCAACGGGAC CCCCACAAAG CGGCGAGCCA TCGGCTTCAA GAAGCTAGCA
    GAAGCTGTCA AGTTCTCGGC CAAGCTGATG GGGCAGGCTA TGGCCAAGAG GGTCAAAGCG
    ACCATCCTCT ATGCCACAGA GACAGGCAAA TCGCAAGCTT ATGCCAAGAC CTTGTGTGAG
    ATCTTCAAAC ACGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATCGTGCAC
    CTGGAACATG AAACTCTGGT CCTCGTGGTC ACCAGCACCT TTGGCAATGG AGATCCCCCT
    GAGAATGGGG AGAAATTCGG CTGTGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAG
    GAAGAAAGGA AGTACCCGGA ACCCTTGCGT TTCTTTCCCC GTAAAGGGCC TCCCCTCCCC
    AATGGTGACA CAGAAGTCCA CGGTCTGGCT GCAGCCCGTG ACAGCCAGCA CAGGAGCTAC
    AAGGTCCGAT TCAACAGCGT CTCCTCCTAC TCTGACTCCC AAAAATCATC AGGCGATGGG
    CCCGACCTCA GAGACAACTT TGAGAGTGCT GGACCCCTGG CCAATGTGAG GTTCTCAGTT
    TTTGGCCTCG GCTCACGAGC ATACCCTCAC TTTTGTGCCT TCGGACACGC TGTGGACACC
    CTCCTGGAAG AACTGGGAGG GGAGAGGATC CTGAAGATGA GGGAAGGGGA TGAGCTCTGT
    GGGCAGGAAG AGGCTTTCAG GACCTGGGCC AAGAAGGTCT TCAAGGCAGC CTGTGATGTC
    TTCTGTGTGG GAGATGATGT CAACATTGAA AAGGCCAACA ATTCCCTCAT CAGCAATGAT
    CGCAGCTGGA AGAGAAACAA GTTCCGCCTC ACCTTTGTGG CCGAAGCTCC AGAACTCACA
    CAAGGTCTAT CCAATGTCCA CAAAAAGCGA GTCTCAGCTG CCCGGCTCCT TAGCCGTCAA
    AACCTCCAGA GTCCTAAATC CAGTCGGTCA ACTATCTTCG TGCGTCTCCA CACCAACGGG
    AACCAGGAGC TGCAGTACCA GCCTGGGGAC CACCTGGGTG TCTTCCCTGG CAACCACGAG
    GACCTCGTGA ATGCCCTGAT CGAGCGGCTG GAGGACGCGC CCCCTGTCAA CCAGATGGTG
    AAAGTGGAAC TGCTGGAGGA GCGGAACACG GCTTTAGGTG TCATCAGTAA CTGGACAGAC
    GAGCTCCGCC TCCCGCCCTG CACCATCTTC CAGGCCTTCA AGTACTACCT GGACATCACC
    ACGCCACCAA CGCCCCTGCA GCTGCAGCAG TTTGCCTCCC TAGCTACCAG CGAGAAGGAG
    AAGCAGCGTC TGCTGGTCCT CAGCAAGGGT TTGCAGGAGT ACGAGGAATG GAAATGGGGC
    AAGAACCCCA CCATCGTGGA GGTGCTGGAG GAATTCCCAT CTATCCAGAT GCCGGCCACC
    CTGCTCCTGA CCCAGCTGTC CCTGCTGCAG CCCCGCTACT ATTCCATCAG CTCCTCCCCA
    GACATGTACC CTGATGAAGT GCACCTCACT GTGGCCATCG TTTCCTACCG CACTCGAGAT
    GGAGAAGGAC CAATTCACCA CGGCGTATGC TCCTCCTGGC TCAACCGGAT ACAGGCTGAC
    GAACTGGTCC CCTGTTTCGT GAGAGGAGCA CCCAGCTTCC ACCTGCCCCG GAACCCCCAA
    GTCCCCTGCA TCCTCGTTGG ACCAGGCACC GGCATTGCCC CTTTCCGAAG CTTCTGGCAA
    CAGCGGCAAT TTGATATCCA ACACAAAGGA ATGAACCCCT GCCCCATGGT CCTGGTCTTC
    GGGTGCCGGC AATCCAAGAT AGATCATATC TACAGGGAAG AGACCCTGCA GGCCAAGAAC
    AAGGGGGTCT TCAGAGAGCT GTACACGGCT TACTCCCGGG AGCCAGACAA ACCAAAGAAG
    TATGTGCAGG ACATCCTGCA GGAGCAGCTG GCGGAGTCTG TGTACCGAGC CCTGAAGGAG
    CAAGGGGGCC ACATATACGT CTGTGGGGAC GTCACCATGG CTGCTGATGT CCTCAAAGCC
    ATCCAGCGCA TCATGACCCA GCAGGGGAAG CTCTCGGCAG AGGACGCCGG CGTATTCATC
    AGCCGGATGA GGGATGACAA CCGATACCAT GAGGATATTT TTGGAGTCAC CCTGCGAACG
    TACGAAGTGA CCAACCGCCT TAGATCTGAG TCCATTGCCT TCATTGAAGA GAGCAAAAAA
    GACACCGATG AGGTTTTCAG CTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc66615
    Clone ID Related Accession (Same CDS sequence) XM_016924331.1 , XM_016924327.2 , XM_016924331.2 , XM_016924328.1 , XM_016924332.2 , XM_016924327.1 , XM_016924332.1 , XM_016924328.2 , XM_016924329.2 , XM_016924329.1
    Accession Version XM_016924327.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4407bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-01
    Organism Pan troglodytes(chimpanzee)
    Product nitric oxide synthase, brain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006479.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAGGATC ACATGTTCGG TGTTCAGCAA ATCCAGCCCA ATGTCATTTC TGTTCGTCTC 
    TTCAAGCGCA AAGTTGGGGG CCTGGGATTC CTGGTGAAGG AGCGGGTCAG TAAGCCGCCC
    GTGATTATCT CTGACCTGAT TCGTGGGGGC GCCGCAGAGC AGAGTGGCCT CATCCAGGCC
    GGAGACATCA TTCTTGCGGT CAACGGCCGG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC
    CTGGAGGTAC TCAGAGGCAT TGCCTCCGAG ACCCACGTGG TCCTCATTCT GAGGGGTCCT
    GAAGGTTTCA CCACGCACCT GGAGACCACC TTTACAGGTG ATGGGACCCC CAAGACCATC
    CGGGTGACAC AGCCCCTGGG TCCCCCCACC AAAGCCGTGG ATCTGTCCCA CCAGCCATCG
    GCCGGCAAAG AACAGCCGCT GGCAGTGGAT GGGGCCTCGG GTCCCGGGAA TGGGCCTCAG
    CATGCCTACG ATGACGGGCA GGAGGCTGGC TCACTCCCCC ATGCCAACGG CCTGGCCCCC
    AGGCCCCCAG GCCAGGACCC TGCGAAGAAA GCAACCAGAG TCAGCCTCCA AGGCAGAGGG
    GAGAACAATG AACTGCTCAA GGAGATAGAG CCTGTGCTGA GCCTTCTCAC CAGTGGGAGC
    AGAGGGGTCA AGGGAGGGGC ACCTGCCAAG GCAGAGATGA AAGATATGGG AATCCAGGTG
    GACAGAGATT TGGACGGCAA GTCACACAAA CCTCTGCCCC TCGCCGTGGA GAATGACCGA
    GTCTTCAATG ACCTATGGGG GAAGGGCAAT GTGCCTGTCG TCCTCAACAA CCCATATTCA
    GAGAAGGAGC AGCCCCCTGC CTCAGGAAAA CAGTCCCCCA CAAAGAATGG CAGCCCCTCC
    AAGTGTCCAC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG AGGTGGTTCT CACTGACACC
    CTCCACCTTA AGAGCACATT GGAAACGGGA TGCACTGAGT ACATCTGCAT GGGCTCCATC
    ATGCATCCTT CTCAGCATGC AAGGAGGCCT GAAGATGTCC GCACAAAAGA ACAGCTCTTC
    CCTCTCGCCA AAGAGTTTAT TGATCAATAC TATTCATCAA TTAAAAGATT TGGCTCCAAA
    GCCCACATGG AAAGGCTGGA AGAGGTGAAC AAAGAGATCG AAACCACTAG CACCTACCAG
    CTCAAGGACA CAGAGCTCAT CTATGGGGCC AAGCACGCCT GGCGGAATGC CTCGCGCTGT
    GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTATTCGATG CCCGTGACTG CACCACGGCC
    CACGGGATGT TCAACTACAT CTGTAACCAC GTCAAGTATG CCACCAACAA AGGGAACCTC
    AGGTCCGCCA TCACCATATT CCCCCAGAGG ACAGACGGCA AGCACGACTT CCGAGTCTGG
    AACTCCCAGC TCATCCGCTA CGCTGGCTAC AAGCAGCCCG ACGGCTCCAC CCTGGGGGAC
    CCAGCCAATG TGCAGTTCAC AGAGATATGC ATACAGCAGG GCTGGAAACC GCCTAGAGGC
    CGCTTCGATG TCCTGCCGCT CCTGCTTCAG GCCAACGGCA ATGACCCTGA GCTCTTCCAG
    ATTCCTCCAG AGCTGGTGTT GGAAGTTCCC ATCAGGCACC CCAAGTTTGA GTGGTTCAAG
    GACCTGGGGC TGAAGTGGTA CGGCCTCCCC GCCGTGTCCA ACATGCTCCT AGAGATTGGC
    GGCCTGGAGT TCAGCGCCTG TCCCTTCAGT GGCTGGTACA TGGGCACAGA GATTGGTGTC
    CGCGACTACT GTGACAACTC CCGCTACAAT ATCCTGGAGG AAGTGGCCAA GAAGATGAAC
    TTAGACATGA GGAAGATGTC CTCCCTGTGG AAGGACCAGG CGCTGGTGGA GATCAATATC
    GCGGTTCTCT ACAGCTTCCA GAGTGACAAA GTGACCATTG TTGACCATCA CTCCGCCACC
    GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGGGGCTG CCCTGCCGAC
    TGGGTGTGGA TCGTGCCCCC CATGTCCGGA AGCATCACCC CTGTGTTCCA CCAGGAGATG
    CTCAACTACC GGCTCACCCC CTCCTTCGAA TACCAGCCTG ATCCCTGGAA CACGCATGTC
    TGGAAAGGCA CCAACGGGAC CCCCACAAAG CGGCGAGCCA TCGGCTTCAA GAAGCTAGCA
    GAAGCTGTCA AGTTCTCGGC CAAGCTGATG GGGCAGGCTA TGGCCAAGAG GGTCAAAGCG
    ACCATCCTCT ATGCCACAGA GACAGGCAAA TCGCAAGCTT ATGCCAAGAC CTTGTGTGAG
    ATCTTCAAAC ACGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATCGTGCAC
    CTGGAACATG AAACTCTGGT CCTCGTGGTC ACCAGCACCT TTGGCAATGG AGATCCCCCT
    GAGAATGGGG AGAAATTCGG CTGTGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAG
    GAAGAAAGGA AGTACCCGGA ACCCTTGCGT TTCTTTCCCC GTAAAGGGCC TCCCCTCCCC
    AATGGTGACA CAGAAGTCCA CGGTCTGGCT GCAGCCCGTG ACAGCCAGCA CAGGAGCTAC
    AAGGTCCGAT TCAACAGCGT CTCCTCCTAC TCTGACTCCC AAAAATCATC AGGCGATGGG
    CCCGACCTCA GAGACAACTT TGAGAGTGCT GGACCCCTGG CCAATGTGAG GTTCTCAGTT
    TTTGGCCTCG GCTCACGAGC ATACCCTCAC TTTTGTGCCT TCGGACACGC TGTGGACACC
    CTCCTGGAAG AACTGGGAGG GGAGAGGATC CTGAAGATGA GGGAAGGGGA TGAGCTCTGT
    GGGCAGGAAG AGGCTTTCAG GACCTGGGCC AAGAAGGTCT TCAAGGCAGC CTGTGATGTC
    TTCTGTGTGG GAGATGATGT CAACATTGAA AAGGCCAACA ATTCCCTCAT CAGCAATGAT
    CGCAGCTGGA AGAGAAACAA GTTCCGCCTC ACCTTTGTGG CCGAAGCTCC AGAACTCACA
    CAAGGTCTAT CCAATGTCCA CAAAAAGCGA GTCTCAGCTG CCCGGCTCCT TAGCCGTCAA
    AACCTCCAGA GTCCTAAATC CAGTCGGTCA ACTATCTTCG TGCGTCTCCA CACCAACGGG
    AACCAGGAGC TGCAGTACCA GCCTGGGGAC CACCTGGGTG TCTTCCCTGG CAACCACGAG
    GACCTCGTGA ATGCCCTGAT CGAGCGGCTG GAGGACGCGC CCCCTGTCAA CCAGATGGTG
    AAAGTGGAAC TGCTGGAGGA GCGGAACACG GCTTTAGGTG TCATCAGTAA CTGGACAGAC
    GAGCTCCGCC TCCCGCCCTG CACCATCTTC CAGGCCTTCA AGTACTACCT GGACATCACC
    ACGCCACCAA CGCCCCTGCA GCTGCAGCAG TTTGCCTCCC TAGCTACCAG CGAGAAGGAG
    AAGCAGCGTC TGCTGGTCCT CAGCAAGGGT TTGCAGGAGT ACGAGGAATG GAAATGGGGC
    AAGAACCCCA CCATCGTGGA GGTGCTGGAG GAATTCCCAT CTATCCAGAT GCCGGCCACC
    CTGCTCCTGA CCCAGCTGTC CCTGCTGCAG CCCCGCTACT ATTCCATCAG CTCCTCCCCA
    GACATGTACC CTGATGAAGT GCACCTCACT GTGGCCATCG TTTCCTACCG CACTCGAGAT
    GGAGAAGGAC CAATTCACCA CGGCGTATGC TCCTCCTGGC TCAACCGGAT ACAGGCTGAC
    GAACTGGTCC CCTGTTTCGT GAGAGGAGCA CCCAGCTTCC ACCTGCCCCG GAACCCCCAA
    GTCCCCTGCA TCCTCGTTGG ACCAGGCACC GGCATTGCCC CTTTCCGAAG CTTCTGGCAA
    CAGCGGCAAT TTGATATCCA ACACAAAGGA ATGAACCCCT GCCCCATGGT CCTGGTCTTC
    GGGTGCCGGC AATCCAAGAT AGATCATATC TACAGGGAAG AGACCCTGCA GGCCAAGAAC
    AAGGGGGTCT TCAGAGAGCT GTACACGGCT TACTCCCGGG AGCCAGACAA ACCAAAGAAG
    TATGTGCAGG ACATCCTGCA GGAGCAGCTG GCGGAGTCTG TGTACCGAGC CCTGAAGGAG
    CAAGGGGGCC ACATATACGT CTGTGGGGAC GTCACCATGG CTGCTGATGT CCTCAAAGCC
    ATCCAGCGCA TCATGACCCA GCAGGGGAAG CTCTCGGCAG AGGACGCCGG CGTATTCATC
    AGCCGGATGA GGGATGACAA CCGATACCAT GAGGATATTT TTGGAGTCAC CCTGCGAACG
    TACGAAGTGA CCAACCGCCT TAGATCTGAG TCCATTGCCT TCATTGAAGA GAGCAAAAAA
    GACACCGATG AGGTTTTCAG CTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016779816.1
    CDS2028..6434
    Translation

    Target ORF information:

    RefSeq Version XM_016924327.1
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes nitric oxide synthase 1 (NOS1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_016924327.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAGGATC ACATGTTCGG TGTTCAGCAA ATCCAGCCCA ATGTCATTTC TGTTCGTCTC 
    TTCAAGCGCA AAGTTGGGGG CCTGGGATTC CTGGTGAAGG AGCGGGTCAG TAAGCCGCCC
    GTGATTATCT CTGACCTGAT TCGTGGGGGC GCCGCAGAGC AGAGTGGCCT CATCCAGGCC
    GGAGACATCA TTCTTGCGGT CAACGGCCGG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC
    CTGGAGGTAC TCAGAGGCAT TGCCTCCGAG ACCCACGTGG TCCTCATTCT GAGGGGTCCT
    GAAGGTTTCA CCACGCACCT GGAGACCACC TTTACAGGTG ATGGGACCCC CAAGACCATC
    CGGGTGACAC AGCCCCTGGG TCCCCCCACC AAAGCCGTGG ATCTGTCCCA CCAGCCATCG
    GCCGGCAAAG AACAGCCGCT GGCAGTGGAT GGGGCCTCGG GTCCCGGGAA TGGGCCTCAG
    CATGCCTACG ATGACGGGCA GGAGGCTGGC TCACTCCCCC ATGCCAACGG CCTGGCCCCC
    AGGCCCCCAG GCCAGGACCC TGCGAAGAAA GCAACCAGAG TCAGCCTCCA AGGCAGAGGG
    GAGAACAATG AACTGCTCAA GGAGATAGAG CCTGTGCTGA GCCTTCTCAC CAGTGGGAGC
    AGAGGGGTCA AGGGAGGGGC ACCTGCCAAG GCAGAGATGA AAGATATGGG AATCCAGGTG
    GACAGAGATT TGGACGGCAA GTCACACAAA CCTCTGCCCC TCGCCGTGGA GAATGACCGA
    GTCTTCAATG ACCTATGGGG GAAGGGCAAT GTGCCTGTCG TCCTCAACAA CCCATATTCA
    GAGAAGGAGC AGCCCCCTGC CTCAGGAAAA CAGTCCCCCA CAAAGAATGG CAGCCCCTCC
    AAGTGTCCAC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG AGGTGGTTCT CACTGACACC
    CTCCACCTTA AGAGCACATT GGAAACGGGA TGCACTGAGT ACATCTGCAT GGGCTCCATC
    ATGCATCCTT CTCAGCATGC AAGGAGGCCT GAAGATGTCC GCACAAAAGA ACAGCTCTTC
    CCTCTCGCCA AAGAGTTTAT TGATCAATAC TATTCATCAA TTAAAAGATT TGGCTCCAAA
    GCCCACATGG AAAGGCTGGA AGAGGTGAAC AAAGAGATCG AAACCACTAG CACCTACCAG
    CTCAAGGACA CAGAGCTCAT CTATGGGGCC AAGCACGCCT GGCGGAATGC CTCGCGCTGT
    GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTATTCGATG CCCGTGACTG CACCACGGCC
    CACGGGATGT TCAACTACAT CTGTAACCAC GTCAAGTATG CCACCAACAA AGGGAACCTC
    AGGTCCGCCA TCACCATATT CCCCCAGAGG ACAGACGGCA AGCACGACTT CCGAGTCTGG
    AACTCCCAGC TCATCCGCTA CGCTGGCTAC AAGCAGCCCG ACGGCTCCAC CCTGGGGGAC
    CCAGCCAATG TGCAGTTCAC AGAGATATGC ATACAGCAGG GCTGGAAACC GCCTAGAGGC
    CGCTTCGATG TCCTGCCGCT CCTGCTTCAG GCCAACGGCA ATGACCCTGA GCTCTTCCAG
    ATTCCTCCAG AGCTGGTGTT GGAAGTTCCC ATCAGGCACC CCAAGTTTGA GTGGTTCAAG
    GACCTGGGGC TGAAGTGGTA CGGCCTCCCC GCCGTGTCCA ACATGCTCCT AGAGATTGGC
    GGCCTGGAGT TCAGCGCCTG TCCCTTCAGT GGCTGGTACA TGGGCACAGA GATTGGTGTC
    CGCGACTACT GTGACAACTC CCGCTACAAT ATCCTGGAGG AAGTGGCCAA GAAGATGAAC
    TTAGACATGA GGAAGATGTC CTCCCTGTGG AAGGACCAGG CGCTGGTGGA GATCAATATC
    GCGGTTCTCT ACAGCTTCCA GAGTGACAAA GTGACCATTG TTGACCATCA CTCCGCCACC
    GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGGGGCTG CCCTGCCGAC
    TGGGTGTGGA TCGTGCCCCC CATGTCCGGA AGCATCACCC CTGTGTTCCA CCAGGAGATG
    CTCAACTACC GGCTCACCCC CTCCTTCGAA TACCAGCCTG ATCCCTGGAA CACGCATGTC
    TGGAAAGGCA CCAACGGGAC CCCCACAAAG CGGCGAGCCA TCGGCTTCAA GAAGCTAGCA
    GAAGCTGTCA AGTTCTCGGC CAAGCTGATG GGGCAGGCTA TGGCCAAGAG GGTCAAAGCG
    ACCATCCTCT ATGCCACAGA GACAGGCAAA TCGCAAGCTT ATGCCAAGAC CTTGTGTGAG
    ATCTTCAAAC ACGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATCGTGCAC
    CTGGAACATG AAACTCTGGT CCTCGTGGTC ACCAGCACCT TTGGCAATGG AGATCCCCCT
    GAGAATGGGG AGAAATTCGG CTGTGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAG
    GAAGAAAGGA AGTACCCGGA ACCCTTGCGT TTCTTTCCCC GTAAAGGGCC TCCCCTCCCC
    AATGGTGACA CAGAAGTCCA CGGTCTGGCT GCAGCCCGTG ACAGCCAGCA CAGGAGCTAC
    AAGGTCCGAT TCAACAGCGT CTCCTCCTAC TCTGACTCCC AAAAATCATC AGGCGATGGG
    CCCGACCTCA GAGACAACTT TGAGAGTGCT GGACCCCTGG CCAATGTGAG GTTCTCAGTT
    TTTGGCCTCG GCTCACGAGC ATACCCTCAC TTTTGTGCCT TCGGACACGC TGTGGACACC
    CTCCTGGAAG AACTGGGAGG GGAGAGGATC CTGAAGATGA GGGAAGGGGA TGAGCTCTGT
    GGGCAGGAAG AGGCTTTCAG GACCTGGGCC AAGAAGGTCT TCAAGGCAGC CTGTGATGTC
    TTCTGTGTGG GAGATGATGT CAACATTGAA AAGGCCAACA ATTCCCTCAT CAGCAATGAT
    CGCAGCTGGA AGAGAAACAA GTTCCGCCTC ACCTTTGTGG CCGAAGCTCC AGAACTCACA
    CAAGGTCTAT CCAATGTCCA CAAAAAGCGA GTCTCAGCTG CCCGGCTCCT TAGCCGTCAA
    AACCTCCAGA GTCCTAAATC CAGTCGGTCA ACTATCTTCG TGCGTCTCCA CACCAACGGG
    AACCAGGAGC TGCAGTACCA GCCTGGGGAC CACCTGGGTG TCTTCCCTGG CAACCACGAG
    GACCTCGTGA ATGCCCTGAT CGAGCGGCTG GAGGACGCGC CCCCTGTCAA CCAGATGGTG
    AAAGTGGAAC TGCTGGAGGA GCGGAACACG GCTTTAGGTG TCATCAGTAA CTGGACAGAC
    GAGCTCCGCC TCCCGCCCTG CACCATCTTC CAGGCCTTCA AGTACTACCT GGACATCACC
    ACGCCACCAA CGCCCCTGCA GCTGCAGCAG TTTGCCTCCC TAGCTACCAG CGAGAAGGAG
    AAGCAGCGTC TGCTGGTCCT CAGCAAGGGT TTGCAGGAGT ACGAGGAATG GAAATGGGGC
    AAGAACCCCA CCATCGTGGA GGTGCTGGAG GAATTCCCAT CTATCCAGAT GCCGGCCACC
    CTGCTCCTGA CCCAGCTGTC CCTGCTGCAG CCCCGCTACT ATTCCATCAG CTCCTCCCCA
    GACATGTACC CTGATGAAGT GCACCTCACT GTGGCCATCG TTTCCTACCG CACTCGAGAT
    GGAGAAGGAC CAATTCACCA CGGCGTATGC TCCTCCTGGC TCAACCGGAT ACAGGCTGAC
    GAACTGGTCC CCTGTTTCGT GAGAGGAGCA CCCAGCTTCC ACCTGCCCCG GAACCCCCAA
    GTCCCCTGCA TCCTCGTTGG ACCAGGCACC GGCATTGCCC CTTTCCGAAG CTTCTGGCAA
    CAGCGGCAAT TTGATATCCA ACACAAAGGA ATGAACCCCT GCCCCATGGT CCTGGTCTTC
    GGGTGCCGGC AATCCAAGAT AGATCATATC TACAGGGAAG AGACCCTGCA GGCCAAGAAC
    AAGGGGGTCT TCAGAGAGCT GTACACGGCT TACTCCCGGG AGCCAGACAA ACCAAAGAAG
    TATGTGCAGG ACATCCTGCA GGAGCAGCTG GCGGAGTCTG TGTACCGAGC CCTGAAGGAG
    CAAGGGGGCC ACATATACGT CTGTGGGGAC GTCACCATGG CTGCTGATGT CCTCAAAGCC
    ATCCAGCGCA TCATGACCCA GCAGGGGAAG CTCTCGGCAG AGGACGCCGG CGTATTCATC
    AGCCGGATGA GGGATGACAA CCGATACCAT GAGGATATTT TTGGAGTCAC CCTGCGAACG
    TACGAAGTGA CCAACCGCCT TAGATCTGAG TCCATTGCCT TCATTGAAGA GAGCAAAAAA
    GACACCGATG AGGTTTTCAG CTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc66615
    Clone ID Related Accession (Same CDS sequence) XM_016924331.1 , XM_016924327.2 , XM_016924331.2 , XM_016924328.1 , XM_016924332.2 , XM_016924327.1 , XM_016924332.1 , XM_016924328.2 , XM_016924329.2 , XM_016924329.1
    Accession Version XM_016924332.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4407bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-01
    Organism Pan troglodytes(chimpanzee)
    Product nitric oxide synthase, brain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006479.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAGGATC ACATGTTCGG TGTTCAGCAA ATCCAGCCCA ATGTCATTTC TGTTCGTCTC 
    TTCAAGCGCA AAGTTGGGGG CCTGGGATTC CTGGTGAAGG AGCGGGTCAG TAAGCCGCCC
    GTGATTATCT CTGACCTGAT TCGTGGGGGC GCCGCAGAGC AGAGTGGCCT CATCCAGGCC
    GGAGACATCA TTCTTGCGGT CAACGGCCGG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC
    CTGGAGGTAC TCAGAGGCAT TGCCTCCGAG ACCCACGTGG TCCTCATTCT GAGGGGTCCT
    GAAGGTTTCA CCACGCACCT GGAGACCACC TTTACAGGTG ATGGGACCCC CAAGACCATC
    CGGGTGACAC AGCCCCTGGG TCCCCCCACC AAAGCCGTGG ATCTGTCCCA CCAGCCATCG
    GCCGGCAAAG AACAGCCGCT GGCAGTGGAT GGGGCCTCGG GTCCCGGGAA TGGGCCTCAG
    CATGCCTACG ATGACGGGCA GGAGGCTGGC TCACTCCCCC ATGCCAACGG CCTGGCCCCC
    AGGCCCCCAG GCCAGGACCC TGCGAAGAAA GCAACCAGAG TCAGCCTCCA AGGCAGAGGG
    GAGAACAATG AACTGCTCAA GGAGATAGAG CCTGTGCTGA GCCTTCTCAC CAGTGGGAGC
    AGAGGGGTCA AGGGAGGGGC ACCTGCCAAG GCAGAGATGA AAGATATGGG AATCCAGGTG
    GACAGAGATT TGGACGGCAA GTCACACAAA CCTCTGCCCC TCGCCGTGGA GAATGACCGA
    GTCTTCAATG ACCTATGGGG GAAGGGCAAT GTGCCTGTCG TCCTCAACAA CCCATATTCA
    GAGAAGGAGC AGCCCCCTGC CTCAGGAAAA CAGTCCCCCA CAAAGAATGG CAGCCCCTCC
    AAGTGTCCAC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG AGGTGGTTCT CACTGACACC
    CTCCACCTTA AGAGCACATT GGAAACGGGA TGCACTGAGT ACATCTGCAT GGGCTCCATC
    ATGCATCCTT CTCAGCATGC AAGGAGGCCT GAAGATGTCC GCACAAAAGA ACAGCTCTTC
    CCTCTCGCCA AAGAGTTTAT TGATCAATAC TATTCATCAA TTAAAAGATT TGGCTCCAAA
    GCCCACATGG AAAGGCTGGA AGAGGTGAAC AAAGAGATCG AAACCACTAG CACCTACCAG
    CTCAAGGACA CAGAGCTCAT CTATGGGGCC AAGCACGCCT GGCGGAATGC CTCGCGCTGT
    GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTATTCGATG CCCGTGACTG CACCACGGCC
    CACGGGATGT TCAACTACAT CTGTAACCAC GTCAAGTATG CCACCAACAA AGGGAACCTC
    AGGTCCGCCA TCACCATATT CCCCCAGAGG ACAGACGGCA AGCACGACTT CCGAGTCTGG
    AACTCCCAGC TCATCCGCTA CGCTGGCTAC AAGCAGCCCG ACGGCTCCAC CCTGGGGGAC
    CCAGCCAATG TGCAGTTCAC AGAGATATGC ATACAGCAGG GCTGGAAACC GCCTAGAGGC
    CGCTTCGATG TCCTGCCGCT CCTGCTTCAG GCCAACGGCA ATGACCCTGA GCTCTTCCAG
    ATTCCTCCAG AGCTGGTGTT GGAAGTTCCC ATCAGGCACC CCAAGTTTGA GTGGTTCAAG
    GACCTGGGGC TGAAGTGGTA CGGCCTCCCC GCCGTGTCCA ACATGCTCCT AGAGATTGGC
    GGCCTGGAGT TCAGCGCCTG TCCCTTCAGT GGCTGGTACA TGGGCACAGA GATTGGTGTC
    CGCGACTACT GTGACAACTC CCGCTACAAT ATCCTGGAGG AAGTGGCCAA GAAGATGAAC
    TTAGACATGA GGAAGATGTC CTCCCTGTGG AAGGACCAGG CGCTGGTGGA GATCAATATC
    GCGGTTCTCT ACAGCTTCCA GAGTGACAAA GTGACCATTG TTGACCATCA CTCCGCCACC
    GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGGGGCTG CCCTGCCGAC
    TGGGTGTGGA TCGTGCCCCC CATGTCCGGA AGCATCACCC CTGTGTTCCA CCAGGAGATG
    CTCAACTACC GGCTCACCCC CTCCTTCGAA TACCAGCCTG ATCCCTGGAA CACGCATGTC
    TGGAAAGGCA CCAACGGGAC CCCCACAAAG CGGCGAGCCA TCGGCTTCAA GAAGCTAGCA
    GAAGCTGTCA AGTTCTCGGC CAAGCTGATG GGGCAGGCTA TGGCCAAGAG GGTCAAAGCG
    ACCATCCTCT ATGCCACAGA GACAGGCAAA TCGCAAGCTT ATGCCAAGAC CTTGTGTGAG
    ATCTTCAAAC ACGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATCGTGCAC
    CTGGAACATG AAACTCTGGT CCTCGTGGTC ACCAGCACCT TTGGCAATGG AGATCCCCCT
    GAGAATGGGG AGAAATTCGG CTGTGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAG
    GAAGAAAGGA AGTACCCGGA ACCCTTGCGT TTCTTTCCCC GTAAAGGGCC TCCCCTCCCC
    AATGGTGACA CAGAAGTCCA CGGTCTGGCT GCAGCCCGTG ACAGCCAGCA CAGGAGCTAC
    AAGGTCCGAT TCAACAGCGT CTCCTCCTAC TCTGACTCCC AAAAATCATC AGGCGATGGG
    CCCGACCTCA GAGACAACTT TGAGAGTGCT GGACCCCTGG CCAATGTGAG GTTCTCAGTT
    TTTGGCCTCG GCTCACGAGC ATACCCTCAC TTTTGTGCCT TCGGACACGC TGTGGACACC
    CTCCTGGAAG AACTGGGAGG GGAGAGGATC CTGAAGATGA GGGAAGGGGA TGAGCTCTGT
    GGGCAGGAAG AGGCTTTCAG GACCTGGGCC AAGAAGGTCT TCAAGGCAGC CTGTGATGTC
    TTCTGTGTGG GAGATGATGT CAACATTGAA AAGGCCAACA ATTCCCTCAT CAGCAATGAT
    CGCAGCTGGA AGAGAAACAA GTTCCGCCTC ACCTTTGTGG CCGAAGCTCC AGAACTCACA
    CAAGGTCTAT CCAATGTCCA CAAAAAGCGA GTCTCAGCTG CCCGGCTCCT TAGCCGTCAA
    AACCTCCAGA GTCCTAAATC CAGTCGGTCA ACTATCTTCG TGCGTCTCCA CACCAACGGG
    AACCAGGAGC TGCAGTACCA GCCTGGGGAC CACCTGGGTG TCTTCCCTGG CAACCACGAG
    GACCTCGTGA ATGCCCTGAT CGAGCGGCTG GAGGACGCGC CCCCTGTCAA CCAGATGGTG
    AAAGTGGAAC TGCTGGAGGA GCGGAACACG GCTTTAGGTG TCATCAGTAA CTGGACAGAC
    GAGCTCCGCC TCCCGCCCTG CACCATCTTC CAGGCCTTCA AGTACTACCT GGACATCACC
    ACGCCACCAA CGCCCCTGCA GCTGCAGCAG TTTGCCTCCC TAGCTACCAG CGAGAAGGAG
    AAGCAGCGTC TGCTGGTCCT CAGCAAGGGT TTGCAGGAGT ACGAGGAATG GAAATGGGGC
    AAGAACCCCA CCATCGTGGA GGTGCTGGAG GAATTCCCAT CTATCCAGAT GCCGGCCACC
    CTGCTCCTGA CCCAGCTGTC CCTGCTGCAG CCCCGCTACT ATTCCATCAG CTCCTCCCCA
    GACATGTACC CTGATGAAGT GCACCTCACT GTGGCCATCG TTTCCTACCG CACTCGAGAT
    GGAGAAGGAC CAATTCACCA CGGCGTATGC TCCTCCTGGC TCAACCGGAT ACAGGCTGAC
    GAACTGGTCC CCTGTTTCGT GAGAGGAGCA CCCAGCTTCC ACCTGCCCCG GAACCCCCAA
    GTCCCCTGCA TCCTCGTTGG ACCAGGCACC GGCATTGCCC CTTTCCGAAG CTTCTGGCAA
    CAGCGGCAAT TTGATATCCA ACACAAAGGA ATGAACCCCT GCCCCATGGT CCTGGTCTTC
    GGGTGCCGGC AATCCAAGAT AGATCATATC TACAGGGAAG AGACCCTGCA GGCCAAGAAC
    AAGGGGGTCT TCAGAGAGCT GTACACGGCT TACTCCCGGG AGCCAGACAA ACCAAAGAAG
    TATGTGCAGG ACATCCTGCA GGAGCAGCTG GCGGAGTCTG TGTACCGAGC CCTGAAGGAG
    CAAGGGGGCC ACATATACGT CTGTGGGGAC GTCACCATGG CTGCTGATGT CCTCAAAGCC
    ATCCAGCGCA TCATGACCCA GCAGGGGAAG CTCTCGGCAG AGGACGCCGG CGTATTCATC
    AGCCGGATGA GGGATGACAA CCGATACCAT GAGGATATTT TTGGAGTCAC CCTGCGAACG
    TACGAAGTGA CCAACCGCCT TAGATCTGAG TCCATTGCCT TCATTGAAGA GAGCAAAAAA
    GACACCGATG AGGTTTTCAG CTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016779821.1
    CDS506..4912
    Translation

    Target ORF information:

    RefSeq Version XM_016924332.1
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes nitric oxide synthase 1 (NOS1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_016924332.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAGGATC ACATGTTCGG TGTTCAGCAA ATCCAGCCCA ATGTCATTTC TGTTCGTCTC 
    TTCAAGCGCA AAGTTGGGGG CCTGGGATTC CTGGTGAAGG AGCGGGTCAG TAAGCCGCCC
    GTGATTATCT CTGACCTGAT TCGTGGGGGC GCCGCAGAGC AGAGTGGCCT CATCCAGGCC
    GGAGACATCA TTCTTGCGGT CAACGGCCGG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC
    CTGGAGGTAC TCAGAGGCAT TGCCTCCGAG ACCCACGTGG TCCTCATTCT GAGGGGTCCT
    GAAGGTTTCA CCACGCACCT GGAGACCACC TTTACAGGTG ATGGGACCCC CAAGACCATC
    CGGGTGACAC AGCCCCTGGG TCCCCCCACC AAAGCCGTGG ATCTGTCCCA CCAGCCATCG
    GCCGGCAAAG AACAGCCGCT GGCAGTGGAT GGGGCCTCGG GTCCCGGGAA TGGGCCTCAG
    CATGCCTACG ATGACGGGCA GGAGGCTGGC TCACTCCCCC ATGCCAACGG CCTGGCCCCC
    AGGCCCCCAG GCCAGGACCC TGCGAAGAAA GCAACCAGAG TCAGCCTCCA AGGCAGAGGG
    GAGAACAATG AACTGCTCAA GGAGATAGAG CCTGTGCTGA GCCTTCTCAC CAGTGGGAGC
    AGAGGGGTCA AGGGAGGGGC ACCTGCCAAG GCAGAGATGA AAGATATGGG AATCCAGGTG
    GACAGAGATT TGGACGGCAA GTCACACAAA CCTCTGCCCC TCGCCGTGGA GAATGACCGA
    GTCTTCAATG ACCTATGGGG GAAGGGCAAT GTGCCTGTCG TCCTCAACAA CCCATATTCA
    GAGAAGGAGC AGCCCCCTGC CTCAGGAAAA CAGTCCCCCA CAAAGAATGG CAGCCCCTCC
    AAGTGTCCAC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG AGGTGGTTCT CACTGACACC
    CTCCACCTTA AGAGCACATT GGAAACGGGA TGCACTGAGT ACATCTGCAT GGGCTCCATC
    ATGCATCCTT CTCAGCATGC AAGGAGGCCT GAAGATGTCC GCACAAAAGA ACAGCTCTTC
    CCTCTCGCCA AAGAGTTTAT TGATCAATAC TATTCATCAA TTAAAAGATT TGGCTCCAAA
    GCCCACATGG AAAGGCTGGA AGAGGTGAAC AAAGAGATCG AAACCACTAG CACCTACCAG
    CTCAAGGACA CAGAGCTCAT CTATGGGGCC AAGCACGCCT GGCGGAATGC CTCGCGCTGT
    GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTATTCGATG CCCGTGACTG CACCACGGCC
    CACGGGATGT TCAACTACAT CTGTAACCAC GTCAAGTATG CCACCAACAA AGGGAACCTC
    AGGTCCGCCA TCACCATATT CCCCCAGAGG ACAGACGGCA AGCACGACTT CCGAGTCTGG
    AACTCCCAGC TCATCCGCTA CGCTGGCTAC AAGCAGCCCG ACGGCTCCAC CCTGGGGGAC
    CCAGCCAATG TGCAGTTCAC AGAGATATGC ATACAGCAGG GCTGGAAACC GCCTAGAGGC
    CGCTTCGATG TCCTGCCGCT CCTGCTTCAG GCCAACGGCA ATGACCCTGA GCTCTTCCAG
    ATTCCTCCAG AGCTGGTGTT GGAAGTTCCC ATCAGGCACC CCAAGTTTGA GTGGTTCAAG
    GACCTGGGGC TGAAGTGGTA CGGCCTCCCC GCCGTGTCCA ACATGCTCCT AGAGATTGGC
    GGCCTGGAGT TCAGCGCCTG TCCCTTCAGT GGCTGGTACA TGGGCACAGA GATTGGTGTC
    CGCGACTACT GTGACAACTC CCGCTACAAT ATCCTGGAGG AAGTGGCCAA GAAGATGAAC
    TTAGACATGA GGAAGATGTC CTCCCTGTGG AAGGACCAGG CGCTGGTGGA GATCAATATC
    GCGGTTCTCT ACAGCTTCCA GAGTGACAAA GTGACCATTG TTGACCATCA CTCCGCCACC
    GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGGGGCTG CCCTGCCGAC
    TGGGTGTGGA TCGTGCCCCC CATGTCCGGA AGCATCACCC CTGTGTTCCA CCAGGAGATG
    CTCAACTACC GGCTCACCCC CTCCTTCGAA TACCAGCCTG ATCCCTGGAA CACGCATGTC
    TGGAAAGGCA CCAACGGGAC CCCCACAAAG CGGCGAGCCA TCGGCTTCAA GAAGCTAGCA
    GAAGCTGTCA AGTTCTCGGC CAAGCTGATG GGGCAGGCTA TGGCCAAGAG GGTCAAAGCG
    ACCATCCTCT ATGCCACAGA GACAGGCAAA TCGCAAGCTT ATGCCAAGAC CTTGTGTGAG
    ATCTTCAAAC ACGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATCGTGCAC
    CTGGAACATG AAACTCTGGT CCTCGTGGTC ACCAGCACCT TTGGCAATGG AGATCCCCCT
    GAGAATGGGG AGAAATTCGG CTGTGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAG
    GAAGAAAGGA AGTACCCGGA ACCCTTGCGT TTCTTTCCCC GTAAAGGGCC TCCCCTCCCC
    AATGGTGACA CAGAAGTCCA CGGTCTGGCT GCAGCCCGTG ACAGCCAGCA CAGGAGCTAC
    AAGGTCCGAT TCAACAGCGT CTCCTCCTAC TCTGACTCCC AAAAATCATC AGGCGATGGG
    CCCGACCTCA GAGACAACTT TGAGAGTGCT GGACCCCTGG CCAATGTGAG GTTCTCAGTT
    TTTGGCCTCG GCTCACGAGC ATACCCTCAC TTTTGTGCCT TCGGACACGC TGTGGACACC
    CTCCTGGAAG AACTGGGAGG GGAGAGGATC CTGAAGATGA GGGAAGGGGA TGAGCTCTGT
    GGGCAGGAAG AGGCTTTCAG GACCTGGGCC AAGAAGGTCT TCAAGGCAGC CTGTGATGTC
    TTCTGTGTGG GAGATGATGT CAACATTGAA AAGGCCAACA ATTCCCTCAT CAGCAATGAT
    CGCAGCTGGA AGAGAAACAA GTTCCGCCTC ACCTTTGTGG CCGAAGCTCC AGAACTCACA
    CAAGGTCTAT CCAATGTCCA CAAAAAGCGA GTCTCAGCTG CCCGGCTCCT TAGCCGTCAA
    AACCTCCAGA GTCCTAAATC CAGTCGGTCA ACTATCTTCG TGCGTCTCCA CACCAACGGG
    AACCAGGAGC TGCAGTACCA GCCTGGGGAC CACCTGGGTG TCTTCCCTGG CAACCACGAG
    GACCTCGTGA ATGCCCTGAT CGAGCGGCTG GAGGACGCGC CCCCTGTCAA CCAGATGGTG
    AAAGTGGAAC TGCTGGAGGA GCGGAACACG GCTTTAGGTG TCATCAGTAA CTGGACAGAC
    GAGCTCCGCC TCCCGCCCTG CACCATCTTC CAGGCCTTCA AGTACTACCT GGACATCACC
    ACGCCACCAA CGCCCCTGCA GCTGCAGCAG TTTGCCTCCC TAGCTACCAG CGAGAAGGAG
    AAGCAGCGTC TGCTGGTCCT CAGCAAGGGT TTGCAGGAGT ACGAGGAATG GAAATGGGGC
    AAGAACCCCA CCATCGTGGA GGTGCTGGAG GAATTCCCAT CTATCCAGAT GCCGGCCACC
    CTGCTCCTGA CCCAGCTGTC CCTGCTGCAG CCCCGCTACT ATTCCATCAG CTCCTCCCCA
    GACATGTACC CTGATGAAGT GCACCTCACT GTGGCCATCG TTTCCTACCG CACTCGAGAT
    GGAGAAGGAC CAATTCACCA CGGCGTATGC TCCTCCTGGC TCAACCGGAT ACAGGCTGAC
    GAACTGGTCC CCTGTTTCGT GAGAGGAGCA CCCAGCTTCC ACCTGCCCCG GAACCCCCAA
    GTCCCCTGCA TCCTCGTTGG ACCAGGCACC GGCATTGCCC CTTTCCGAAG CTTCTGGCAA
    CAGCGGCAAT TTGATATCCA ACACAAAGGA ATGAACCCCT GCCCCATGGT CCTGGTCTTC
    GGGTGCCGGC AATCCAAGAT AGATCATATC TACAGGGAAG AGACCCTGCA GGCCAAGAAC
    AAGGGGGTCT TCAGAGAGCT GTACACGGCT TACTCCCGGG AGCCAGACAA ACCAAAGAAG
    TATGTGCAGG ACATCCTGCA GGAGCAGCTG GCGGAGTCTG TGTACCGAGC CCTGAAGGAG
    CAAGGGGGCC ACATATACGT CTGTGGGGAC GTCACCATGG CTGCTGATGT CCTCAAAGCC
    ATCCAGCGCA TCATGACCCA GCAGGGGAAG CTCTCGGCAG AGGACGCCGG CGTATTCATC
    AGCCGGATGA GGGATGACAA CCGATACCAT GAGGATATTT TTGGAGTCAC CCTGCGAACG
    TACGAAGTGA CCAACCGCCT TAGATCTGAG TCCATTGCCT TCATTGAAGA GAGCAAAAAA
    GACACCGATG AGGTTTTCAG CTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc66615
    Clone ID Related Accession (Same CDS sequence) XM_016924331.1 , XM_016924327.2 , XM_016924331.2 , XM_016924328.1 , XM_016924332.2 , XM_016924327.1 , XM_016924332.1 , XM_016924328.2 , XM_016924329.2 , XM_016924329.1
    Accession Version XM_016924328.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4407bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-19
    Organism Pan troglodytes(chimpanzee)
    Product nitric oxide synthase, brain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036891.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016924328.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAGGATC ACATGTTCGG TGTTCAGCAA ATCCAGCCCA ATGTCATTTC TGTTCGTCTC 
    TTCAAGCGCA AAGTTGGGGG CCTGGGATTC CTGGTGAAGG AGCGGGTCAG TAAGCCGCCC
    GTGATTATCT CTGACCTGAT TCGTGGGGGC GCCGCAGAGC AGAGTGGCCT CATCCAGGCC
    GGAGACATCA TTCTTGCGGT CAACGGCCGG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC
    CTGGAGGTAC TCAGAGGCAT TGCCTCCGAG ACCCACGTGG TCCTCATTCT GAGGGGTCCT
    GAAGGTTTCA CCACGCACCT GGAGACCACC TTTACAGGTG ATGGGACCCC CAAGACCATC
    CGGGTGACAC AGCCCCTGGG TCCCCCCACC AAAGCCGTGG ATCTGTCCCA CCAGCCATCG
    GCCGGCAAAG AACAGCCGCT GGCAGTGGAT GGGGCCTCGG GTCCCGGGAA TGGGCCTCAG
    CATGCCTACG ATGACGGGCA GGAGGCTGGC TCACTCCCCC ATGCCAACGG CCTGGCCCCC
    AGGCCCCCAG GCCAGGACCC TGCGAAGAAA GCAACCAGAG TCAGCCTCCA AGGCAGAGGG
    GAGAACAATG AACTGCTCAA GGAGATAGAG CCTGTGCTGA GCCTTCTCAC CAGTGGGAGC
    AGAGGGGTCA AGGGAGGGGC ACCTGCCAAG GCAGAGATGA AAGATATGGG AATCCAGGTG
    GACAGAGATT TGGACGGCAA GTCACACAAA CCTCTGCCCC TCGCCGTGGA GAATGACCGA
    GTCTTCAATG ACCTATGGGG GAAGGGCAAT GTGCCTGTCG TCCTCAACAA CCCATATTCA
    GAGAAGGAGC AGCCCCCTGC CTCAGGAAAA CAGTCCCCCA CAAAGAATGG CAGCCCCTCC
    AAGTGTCCAC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG AGGTGGTTCT CACTGACACC
    CTCCACCTTA AGAGCACATT GGAAACGGGA TGCACTGAGT ACATCTGCAT GGGCTCCATC
    ATGCATCCTT CTCAGCATGC AAGGAGGCCT GAAGATGTCC GCACAAAAGA ACAGCTCTTC
    CCTCTCGCCA AAGAGTTTAT TGATCAATAC TATTCATCAA TTAAAAGATT TGGCTCCAAA
    GCCCACATGG AAAGGCTGGA AGAGGTGAAC AAAGAGATCG AAACCACTAG CACCTACCAG
    CTCAAGGACA CAGAGCTCAT CTATGGGGCC AAGCACGCCT GGCGGAATGC CTCGCGCTGT
    GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTATTCGATG CCCGTGACTG CACCACGGCC
    CACGGGATGT TCAACTACAT CTGTAACCAC GTCAAGTATG CCACCAACAA AGGGAACCTC
    AGGTCCGCCA TCACCATATT CCCCCAGAGG ACAGACGGCA AGCACGACTT CCGAGTCTGG
    AACTCCCAGC TCATCCGCTA CGCTGGCTAC AAGCAGCCCG ACGGCTCCAC CCTGGGGGAC
    CCAGCCAATG TGCAGTTCAC AGAGATATGC ATACAGCAGG GCTGGAAACC GCCTAGAGGC
    CGCTTCGATG TCCTGCCGCT CCTGCTTCAG GCCAACGGCA ATGACCCTGA GCTCTTCCAG
    ATTCCTCCAG AGCTGGTGTT GGAAGTTCCC ATCAGGCACC CCAAGTTTGA GTGGTTCAAG
    GACCTGGGGC TGAAGTGGTA CGGCCTCCCC GCCGTGTCCA ACATGCTCCT AGAGATTGGC
    GGCCTGGAGT TCAGCGCCTG TCCCTTCAGT GGCTGGTACA TGGGCACAGA GATTGGTGTC
    CGCGACTACT GTGACAACTC CCGCTACAAT ATCCTGGAGG AAGTGGCCAA GAAGATGAAC
    TTAGACATGA GGAAGATGTC CTCCCTGTGG AAGGACCAGG CGCTGGTGGA GATCAATATC
    GCGGTTCTCT ACAGCTTCCA GAGTGACAAA GTGACCATTG TTGACCATCA CTCCGCCACC
    GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGGGGCTG CCCTGCCGAC
    TGGGTGTGGA TCGTGCCCCC CATGTCCGGA AGCATCACCC CTGTGTTCCA CCAGGAGATG
    CTCAACTACC GGCTCACCCC CTCCTTCGAA TACCAGCCTG ATCCCTGGAA CACGCATGTC
    TGGAAAGGCA CCAACGGGAC CCCCACAAAG CGGCGAGCCA TCGGCTTCAA GAAGCTAGCA
    GAAGCTGTCA AGTTCTCGGC CAAGCTGATG GGGCAGGCTA TGGCCAAGAG GGTCAAAGCG
    ACCATCCTCT ATGCCACAGA GACAGGCAAA TCGCAAGCTT ATGCCAAGAC CTTGTGTGAG
    ATCTTCAAAC ACGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATCGTGCAC
    CTGGAACATG AAACTCTGGT CCTCGTGGTC ACCAGCACCT TTGGCAATGG AGATCCCCCT
    GAGAATGGGG AGAAATTCGG CTGTGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAG
    GAAGAAAGGA AGTACCCGGA ACCCTTGCGT TTCTTTCCCC GTAAAGGGCC TCCCCTCCCC
    AATGGTGACA CAGAAGTCCA CGGTCTGGCT GCAGCCCGTG ACAGCCAGCA CAGGAGCTAC
    AAGGTCCGAT TCAACAGCGT CTCCTCCTAC TCTGACTCCC AAAAATCATC AGGCGATGGG
    CCCGACCTCA GAGACAACTT TGAGAGTGCT GGACCCCTGG CCAATGTGAG GTTCTCAGTT
    TTTGGCCTCG GCTCACGAGC ATACCCTCAC TTTTGTGCCT TCGGACACGC TGTGGACACC
    CTCCTGGAAG AACTGGGAGG GGAGAGGATC CTGAAGATGA GGGAAGGGGA TGAGCTCTGT
    GGGCAGGAAG AGGCTTTCAG GACCTGGGCC AAGAAGGTCT TCAAGGCAGC CTGTGATGTC
    TTCTGTGTGG GAGATGATGT CAACATTGAA AAGGCCAACA ATTCCCTCAT CAGCAATGAT
    CGCAGCTGGA AGAGAAACAA GTTCCGCCTC ACCTTTGTGG CCGAAGCTCC AGAACTCACA
    CAAGGTCTAT CCAATGTCCA CAAAAAGCGA GTCTCAGCTG CCCGGCTCCT TAGCCGTCAA
    AACCTCCAGA GTCCTAAATC CAGTCGGTCA ACTATCTTCG TGCGTCTCCA CACCAACGGG
    AACCAGGAGC TGCAGTACCA GCCTGGGGAC CACCTGGGTG TCTTCCCTGG CAACCACGAG
    GACCTCGTGA ATGCCCTGAT CGAGCGGCTG GAGGACGCGC CCCCTGTCAA CCAGATGGTG
    AAAGTGGAAC TGCTGGAGGA GCGGAACACG GCTTTAGGTG TCATCAGTAA CTGGACAGAC
    GAGCTCCGCC TCCCGCCCTG CACCATCTTC CAGGCCTTCA AGTACTACCT GGACATCACC
    ACGCCACCAA CGCCCCTGCA GCTGCAGCAG TTTGCCTCCC TAGCTACCAG CGAGAAGGAG
    AAGCAGCGTC TGCTGGTCCT CAGCAAGGGT TTGCAGGAGT ACGAGGAATG GAAATGGGGC
    AAGAACCCCA CCATCGTGGA GGTGCTGGAG GAATTCCCAT CTATCCAGAT GCCGGCCACC
    CTGCTCCTGA CCCAGCTGTC CCTGCTGCAG CCCCGCTACT ATTCCATCAG CTCCTCCCCA
    GACATGTACC CTGATGAAGT GCACCTCACT GTGGCCATCG TTTCCTACCG CACTCGAGAT
    GGAGAAGGAC CAATTCACCA CGGCGTATGC TCCTCCTGGC TCAACCGGAT ACAGGCTGAC
    GAACTGGTCC CCTGTTTCGT GAGAGGAGCA CCCAGCTTCC ACCTGCCCCG GAACCCCCAA
    GTCCCCTGCA TCCTCGTTGG ACCAGGCACC GGCATTGCCC CTTTCCGAAG CTTCTGGCAA
    CAGCGGCAAT TTGATATCCA ACACAAAGGA ATGAACCCCT GCCCCATGGT CCTGGTCTTC
    GGGTGCCGGC AATCCAAGAT AGATCATATC TACAGGGAAG AGACCCTGCA GGCCAAGAAC
    AAGGGGGTCT TCAGAGAGCT GTACACGGCT TACTCCCGGG AGCCAGACAA ACCAAAGAAG
    TATGTGCAGG ACATCCTGCA GGAGCAGCTG GCGGAGTCTG TGTACCGAGC CCTGAAGGAG
    CAAGGGGGCC ACATATACGT CTGTGGGGAC GTCACCATGG CTGCTGATGT CCTCAAAGCC
    ATCCAGCGCA TCATGACCCA GCAGGGGAAG CTCTCGGCAG AGGACGCCGG CGTATTCATC
    AGCCGGATGA GGGATGACAA CCGATACCAT GAGGATATTT TTGGAGTCAC CCTGCGAACG
    TACGAAGTGA CCAACCGCCT TAGATCTGAG TCCATTGCCT TCATTGAAGA GAGCAAAAAA
    GACACCGATG AGGTTTTCAG CTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016779817.1
    CDS712..5118
    Translation

    Target ORF information:

    RefSeq Version XM_016924328.2
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes nitric oxide synthase 1 (NOS1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_016924328.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAGGATC ACATGTTCGG TGTTCAGCAA ATCCAGCCCA ATGTCATTTC TGTTCGTCTC 
    TTCAAGCGCA AAGTTGGGGG CCTGGGATTC CTGGTGAAGG AGCGGGTCAG TAAGCCGCCC
    GTGATTATCT CTGACCTGAT TCGTGGGGGC GCCGCAGAGC AGAGTGGCCT CATCCAGGCC
    GGAGACATCA TTCTTGCGGT CAACGGCCGG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC
    CTGGAGGTAC TCAGAGGCAT TGCCTCCGAG ACCCACGTGG TCCTCATTCT GAGGGGTCCT
    GAAGGTTTCA CCACGCACCT GGAGACCACC TTTACAGGTG ATGGGACCCC CAAGACCATC
    CGGGTGACAC AGCCCCTGGG TCCCCCCACC AAAGCCGTGG ATCTGTCCCA CCAGCCATCG
    GCCGGCAAAG AACAGCCGCT GGCAGTGGAT GGGGCCTCGG GTCCCGGGAA TGGGCCTCAG
    CATGCCTACG ATGACGGGCA GGAGGCTGGC TCACTCCCCC ATGCCAACGG CCTGGCCCCC
    AGGCCCCCAG GCCAGGACCC TGCGAAGAAA GCAACCAGAG TCAGCCTCCA AGGCAGAGGG
    GAGAACAATG AACTGCTCAA GGAGATAGAG CCTGTGCTGA GCCTTCTCAC CAGTGGGAGC
    AGAGGGGTCA AGGGAGGGGC ACCTGCCAAG GCAGAGATGA AAGATATGGG AATCCAGGTG
    GACAGAGATT TGGACGGCAA GTCACACAAA CCTCTGCCCC TCGCCGTGGA GAATGACCGA
    GTCTTCAATG ACCTATGGGG GAAGGGCAAT GTGCCTGTCG TCCTCAACAA CCCATATTCA
    GAGAAGGAGC AGCCCCCTGC CTCAGGAAAA CAGTCCCCCA CAAAGAATGG CAGCCCCTCC
    AAGTGTCCAC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG AGGTGGTTCT CACTGACACC
    CTCCACCTTA AGAGCACATT GGAAACGGGA TGCACTGAGT ACATCTGCAT GGGCTCCATC
    ATGCATCCTT CTCAGCATGC AAGGAGGCCT GAAGATGTCC GCACAAAAGA ACAGCTCTTC
    CCTCTCGCCA AAGAGTTTAT TGATCAATAC TATTCATCAA TTAAAAGATT TGGCTCCAAA
    GCCCACATGG AAAGGCTGGA AGAGGTGAAC AAAGAGATCG AAACCACTAG CACCTACCAG
    CTCAAGGACA CAGAGCTCAT CTATGGGGCC AAGCACGCCT GGCGGAATGC CTCGCGCTGT
    GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTATTCGATG CCCGTGACTG CACCACGGCC
    CACGGGATGT TCAACTACAT CTGTAACCAC GTCAAGTATG CCACCAACAA AGGGAACCTC
    AGGTCCGCCA TCACCATATT CCCCCAGAGG ACAGACGGCA AGCACGACTT CCGAGTCTGG
    AACTCCCAGC TCATCCGCTA CGCTGGCTAC AAGCAGCCCG ACGGCTCCAC CCTGGGGGAC
    CCAGCCAATG TGCAGTTCAC AGAGATATGC ATACAGCAGG GCTGGAAACC GCCTAGAGGC
    CGCTTCGATG TCCTGCCGCT CCTGCTTCAG GCCAACGGCA ATGACCCTGA GCTCTTCCAG
    ATTCCTCCAG AGCTGGTGTT GGAAGTTCCC ATCAGGCACC CCAAGTTTGA GTGGTTCAAG
    GACCTGGGGC TGAAGTGGTA CGGCCTCCCC GCCGTGTCCA ACATGCTCCT AGAGATTGGC
    GGCCTGGAGT TCAGCGCCTG TCCCTTCAGT GGCTGGTACA TGGGCACAGA GATTGGTGTC
    CGCGACTACT GTGACAACTC CCGCTACAAT ATCCTGGAGG AAGTGGCCAA GAAGATGAAC
    TTAGACATGA GGAAGATGTC CTCCCTGTGG AAGGACCAGG CGCTGGTGGA GATCAATATC
    GCGGTTCTCT ACAGCTTCCA GAGTGACAAA GTGACCATTG TTGACCATCA CTCCGCCACC
    GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGGGGCTG CCCTGCCGAC
    TGGGTGTGGA TCGTGCCCCC CATGTCCGGA AGCATCACCC CTGTGTTCCA CCAGGAGATG
    CTCAACTACC GGCTCACCCC CTCCTTCGAA TACCAGCCTG ATCCCTGGAA CACGCATGTC
    TGGAAAGGCA CCAACGGGAC CCCCACAAAG CGGCGAGCCA TCGGCTTCAA GAAGCTAGCA
    GAAGCTGTCA AGTTCTCGGC CAAGCTGATG GGGCAGGCTA TGGCCAAGAG GGTCAAAGCG
    ACCATCCTCT ATGCCACAGA GACAGGCAAA TCGCAAGCTT ATGCCAAGAC CTTGTGTGAG
    ATCTTCAAAC ACGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATCGTGCAC
    CTGGAACATG AAACTCTGGT CCTCGTGGTC ACCAGCACCT TTGGCAATGG AGATCCCCCT
    GAGAATGGGG AGAAATTCGG CTGTGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAG
    GAAGAAAGGA AGTACCCGGA ACCCTTGCGT TTCTTTCCCC GTAAAGGGCC TCCCCTCCCC
    AATGGTGACA CAGAAGTCCA CGGTCTGGCT GCAGCCCGTG ACAGCCAGCA CAGGAGCTAC
    AAGGTCCGAT TCAACAGCGT CTCCTCCTAC TCTGACTCCC AAAAATCATC AGGCGATGGG
    CCCGACCTCA GAGACAACTT TGAGAGTGCT GGACCCCTGG CCAATGTGAG GTTCTCAGTT
    TTTGGCCTCG GCTCACGAGC ATACCCTCAC TTTTGTGCCT TCGGACACGC TGTGGACACC
    CTCCTGGAAG AACTGGGAGG GGAGAGGATC CTGAAGATGA GGGAAGGGGA TGAGCTCTGT
    GGGCAGGAAG AGGCTTTCAG GACCTGGGCC AAGAAGGTCT TCAAGGCAGC CTGTGATGTC
    TTCTGTGTGG GAGATGATGT CAACATTGAA AAGGCCAACA ATTCCCTCAT CAGCAATGAT
    CGCAGCTGGA AGAGAAACAA GTTCCGCCTC ACCTTTGTGG CCGAAGCTCC AGAACTCACA
    CAAGGTCTAT CCAATGTCCA CAAAAAGCGA GTCTCAGCTG CCCGGCTCCT TAGCCGTCAA
    AACCTCCAGA GTCCTAAATC CAGTCGGTCA ACTATCTTCG TGCGTCTCCA CACCAACGGG
    AACCAGGAGC TGCAGTACCA GCCTGGGGAC CACCTGGGTG TCTTCCCTGG CAACCACGAG
    GACCTCGTGA ATGCCCTGAT CGAGCGGCTG GAGGACGCGC CCCCTGTCAA CCAGATGGTG
    AAAGTGGAAC TGCTGGAGGA GCGGAACACG GCTTTAGGTG TCATCAGTAA CTGGACAGAC
    GAGCTCCGCC TCCCGCCCTG CACCATCTTC CAGGCCTTCA AGTACTACCT GGACATCACC
    ACGCCACCAA CGCCCCTGCA GCTGCAGCAG TTTGCCTCCC TAGCTACCAG CGAGAAGGAG
    AAGCAGCGTC TGCTGGTCCT CAGCAAGGGT TTGCAGGAGT ACGAGGAATG GAAATGGGGC
    AAGAACCCCA CCATCGTGGA GGTGCTGGAG GAATTCCCAT CTATCCAGAT GCCGGCCACC
    CTGCTCCTGA CCCAGCTGTC CCTGCTGCAG CCCCGCTACT ATTCCATCAG CTCCTCCCCA
    GACATGTACC CTGATGAAGT GCACCTCACT GTGGCCATCG TTTCCTACCG CACTCGAGAT
    GGAGAAGGAC CAATTCACCA CGGCGTATGC TCCTCCTGGC TCAACCGGAT ACAGGCTGAC
    GAACTGGTCC CCTGTTTCGT GAGAGGAGCA CCCAGCTTCC ACCTGCCCCG GAACCCCCAA
    GTCCCCTGCA TCCTCGTTGG ACCAGGCACC GGCATTGCCC CTTTCCGAAG CTTCTGGCAA
    CAGCGGCAAT TTGATATCCA ACACAAAGGA ATGAACCCCT GCCCCATGGT CCTGGTCTTC
    GGGTGCCGGC AATCCAAGAT AGATCATATC TACAGGGAAG AGACCCTGCA GGCCAAGAAC
    AAGGGGGTCT TCAGAGAGCT GTACACGGCT TACTCCCGGG AGCCAGACAA ACCAAAGAAG
    TATGTGCAGG ACATCCTGCA GGAGCAGCTG GCGGAGTCTG TGTACCGAGC CCTGAAGGAG
    CAAGGGGGCC ACATATACGT CTGTGGGGAC GTCACCATGG CTGCTGATGT CCTCAAAGCC
    ATCCAGCGCA TCATGACCCA GCAGGGGAAG CTCTCGGCAG AGGACGCCGG CGTATTCATC
    AGCCGGATGA GGGATGACAA CCGATACCAT GAGGATATTT TTGGAGTCAC CCTGCGAACG
    TACGAAGTGA CCAACCGCCT TAGATCTGAG TCCATTGCCT TCATTGAAGA GAGCAAAAAA
    GACACCGATG AGGTTTTCAG CTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc66615
    Clone ID Related Accession (Same CDS sequence) XM_016924331.1 , XM_016924327.2 , XM_016924331.2 , XM_016924328.1 , XM_016924332.2 , XM_016924327.1 , XM_016924332.1 , XM_016924328.2 , XM_016924329.2 , XM_016924329.1
    Accession Version XM_016924329.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4407bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-19
    Organism Pan troglodytes(chimpanzee)
    Product nitric oxide synthase, brain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036891.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016924329.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAGGATC ACATGTTCGG TGTTCAGCAA ATCCAGCCCA ATGTCATTTC TGTTCGTCTC 
    TTCAAGCGCA AAGTTGGGGG CCTGGGATTC CTGGTGAAGG AGCGGGTCAG TAAGCCGCCC
    GTGATTATCT CTGACCTGAT TCGTGGGGGC GCCGCAGAGC AGAGTGGCCT CATCCAGGCC
    GGAGACATCA TTCTTGCGGT CAACGGCCGG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC
    CTGGAGGTAC TCAGAGGCAT TGCCTCCGAG ACCCACGTGG TCCTCATTCT GAGGGGTCCT
    GAAGGTTTCA CCACGCACCT GGAGACCACC TTTACAGGTG ATGGGACCCC CAAGACCATC
    CGGGTGACAC AGCCCCTGGG TCCCCCCACC AAAGCCGTGG ATCTGTCCCA CCAGCCATCG
    GCCGGCAAAG AACAGCCGCT GGCAGTGGAT GGGGCCTCGG GTCCCGGGAA TGGGCCTCAG
    CATGCCTACG ATGACGGGCA GGAGGCTGGC TCACTCCCCC ATGCCAACGG CCTGGCCCCC
    AGGCCCCCAG GCCAGGACCC TGCGAAGAAA GCAACCAGAG TCAGCCTCCA AGGCAGAGGG
    GAGAACAATG AACTGCTCAA GGAGATAGAG CCTGTGCTGA GCCTTCTCAC CAGTGGGAGC
    AGAGGGGTCA AGGGAGGGGC ACCTGCCAAG GCAGAGATGA AAGATATGGG AATCCAGGTG
    GACAGAGATT TGGACGGCAA GTCACACAAA CCTCTGCCCC TCGCCGTGGA GAATGACCGA
    GTCTTCAATG ACCTATGGGG GAAGGGCAAT GTGCCTGTCG TCCTCAACAA CCCATATTCA
    GAGAAGGAGC AGCCCCCTGC CTCAGGAAAA CAGTCCCCCA CAAAGAATGG CAGCCCCTCC
    AAGTGTCCAC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG AGGTGGTTCT CACTGACACC
    CTCCACCTTA AGAGCACATT GGAAACGGGA TGCACTGAGT ACATCTGCAT GGGCTCCATC
    ATGCATCCTT CTCAGCATGC AAGGAGGCCT GAAGATGTCC GCACAAAAGA ACAGCTCTTC
    CCTCTCGCCA AAGAGTTTAT TGATCAATAC TATTCATCAA TTAAAAGATT TGGCTCCAAA
    GCCCACATGG AAAGGCTGGA AGAGGTGAAC AAAGAGATCG AAACCACTAG CACCTACCAG
    CTCAAGGACA CAGAGCTCAT CTATGGGGCC AAGCACGCCT GGCGGAATGC CTCGCGCTGT
    GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTATTCGATG CCCGTGACTG CACCACGGCC
    CACGGGATGT TCAACTACAT CTGTAACCAC GTCAAGTATG CCACCAACAA AGGGAACCTC
    AGGTCCGCCA TCACCATATT CCCCCAGAGG ACAGACGGCA AGCACGACTT CCGAGTCTGG
    AACTCCCAGC TCATCCGCTA CGCTGGCTAC AAGCAGCCCG ACGGCTCCAC CCTGGGGGAC
    CCAGCCAATG TGCAGTTCAC AGAGATATGC ATACAGCAGG GCTGGAAACC GCCTAGAGGC
    CGCTTCGATG TCCTGCCGCT CCTGCTTCAG GCCAACGGCA ATGACCCTGA GCTCTTCCAG
    ATTCCTCCAG AGCTGGTGTT GGAAGTTCCC ATCAGGCACC CCAAGTTTGA GTGGTTCAAG
    GACCTGGGGC TGAAGTGGTA CGGCCTCCCC GCCGTGTCCA ACATGCTCCT AGAGATTGGC
    GGCCTGGAGT TCAGCGCCTG TCCCTTCAGT GGCTGGTACA TGGGCACAGA GATTGGTGTC
    CGCGACTACT GTGACAACTC CCGCTACAAT ATCCTGGAGG AAGTGGCCAA GAAGATGAAC
    TTAGACATGA GGAAGATGTC CTCCCTGTGG AAGGACCAGG CGCTGGTGGA GATCAATATC
    GCGGTTCTCT ACAGCTTCCA GAGTGACAAA GTGACCATTG TTGACCATCA CTCCGCCACC
    GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGGGGCTG CCCTGCCGAC
    TGGGTGTGGA TCGTGCCCCC CATGTCCGGA AGCATCACCC CTGTGTTCCA CCAGGAGATG
    CTCAACTACC GGCTCACCCC CTCCTTCGAA TACCAGCCTG ATCCCTGGAA CACGCATGTC
    TGGAAAGGCA CCAACGGGAC CCCCACAAAG CGGCGAGCCA TCGGCTTCAA GAAGCTAGCA
    GAAGCTGTCA AGTTCTCGGC CAAGCTGATG GGGCAGGCTA TGGCCAAGAG GGTCAAAGCG
    ACCATCCTCT ATGCCACAGA GACAGGCAAA TCGCAAGCTT ATGCCAAGAC CTTGTGTGAG
    ATCTTCAAAC ACGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATCGTGCAC
    CTGGAACATG AAACTCTGGT CCTCGTGGTC ACCAGCACCT TTGGCAATGG AGATCCCCCT
    GAGAATGGGG AGAAATTCGG CTGTGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAG
    GAAGAAAGGA AGTACCCGGA ACCCTTGCGT TTCTTTCCCC GTAAAGGGCC TCCCCTCCCC
    AATGGTGACA CAGAAGTCCA CGGTCTGGCT GCAGCCCGTG ACAGCCAGCA CAGGAGCTAC
    AAGGTCCGAT TCAACAGCGT CTCCTCCTAC TCTGACTCCC AAAAATCATC AGGCGATGGG
    CCCGACCTCA GAGACAACTT TGAGAGTGCT GGACCCCTGG CCAATGTGAG GTTCTCAGTT
    TTTGGCCTCG GCTCACGAGC ATACCCTCAC TTTTGTGCCT TCGGACACGC TGTGGACACC
    CTCCTGGAAG AACTGGGAGG GGAGAGGATC CTGAAGATGA GGGAAGGGGA TGAGCTCTGT
    GGGCAGGAAG AGGCTTTCAG GACCTGGGCC AAGAAGGTCT TCAAGGCAGC CTGTGATGTC
    TTCTGTGTGG GAGATGATGT CAACATTGAA AAGGCCAACA ATTCCCTCAT CAGCAATGAT
    CGCAGCTGGA AGAGAAACAA GTTCCGCCTC ACCTTTGTGG CCGAAGCTCC AGAACTCACA
    CAAGGTCTAT CCAATGTCCA CAAAAAGCGA GTCTCAGCTG CCCGGCTCCT TAGCCGTCAA
    AACCTCCAGA GTCCTAAATC CAGTCGGTCA ACTATCTTCG TGCGTCTCCA CACCAACGGG
    AACCAGGAGC TGCAGTACCA GCCTGGGGAC CACCTGGGTG TCTTCCCTGG CAACCACGAG
    GACCTCGTGA ATGCCCTGAT CGAGCGGCTG GAGGACGCGC CCCCTGTCAA CCAGATGGTG
    AAAGTGGAAC TGCTGGAGGA GCGGAACACG GCTTTAGGTG TCATCAGTAA CTGGACAGAC
    GAGCTCCGCC TCCCGCCCTG CACCATCTTC CAGGCCTTCA AGTACTACCT GGACATCACC
    ACGCCACCAA CGCCCCTGCA GCTGCAGCAG TTTGCCTCCC TAGCTACCAG CGAGAAGGAG
    AAGCAGCGTC TGCTGGTCCT CAGCAAGGGT TTGCAGGAGT ACGAGGAATG GAAATGGGGC
    AAGAACCCCA CCATCGTGGA GGTGCTGGAG GAATTCCCAT CTATCCAGAT GCCGGCCACC
    CTGCTCCTGA CCCAGCTGTC CCTGCTGCAG CCCCGCTACT ATTCCATCAG CTCCTCCCCA
    GACATGTACC CTGATGAAGT GCACCTCACT GTGGCCATCG TTTCCTACCG CACTCGAGAT
    GGAGAAGGAC CAATTCACCA CGGCGTATGC TCCTCCTGGC TCAACCGGAT ACAGGCTGAC
    GAACTGGTCC CCTGTTTCGT GAGAGGAGCA CCCAGCTTCC ACCTGCCCCG GAACCCCCAA
    GTCCCCTGCA TCCTCGTTGG ACCAGGCACC GGCATTGCCC CTTTCCGAAG CTTCTGGCAA
    CAGCGGCAAT TTGATATCCA ACACAAAGGA ATGAACCCCT GCCCCATGGT CCTGGTCTTC
    GGGTGCCGGC AATCCAAGAT AGATCATATC TACAGGGAAG AGACCCTGCA GGCCAAGAAC
    AAGGGGGTCT TCAGAGAGCT GTACACGGCT TACTCCCGGG AGCCAGACAA ACCAAAGAAG
    TATGTGCAGG ACATCCTGCA GGAGCAGCTG GCGGAGTCTG TGTACCGAGC CCTGAAGGAG
    CAAGGGGGCC ACATATACGT CTGTGGGGAC GTCACCATGG CTGCTGATGT CCTCAAAGCC
    ATCCAGCGCA TCATGACCCA GCAGGGGAAG CTCTCGGCAG AGGACGCCGG CGTATTCATC
    AGCCGGATGA GGGATGACAA CCGATACCAT GAGGATATTT TTGGAGTCAC CCTGCGAACG
    TACGAAGTGA CCAACCGCCT TAGATCTGAG TCCATTGCCT TCATTGAAGA GAGCAAAAAA
    GACACCGATG AGGTTTTCAG CTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016779818.1
    CDS2124..6530
    Translation

    Target ORF information:

    RefSeq Version XM_016924329.2
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes nitric oxide synthase 1 (NOS1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_016924329.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAGGATC ACATGTTCGG TGTTCAGCAA ATCCAGCCCA ATGTCATTTC TGTTCGTCTC 
    TTCAAGCGCA AAGTTGGGGG CCTGGGATTC CTGGTGAAGG AGCGGGTCAG TAAGCCGCCC
    GTGATTATCT CTGACCTGAT TCGTGGGGGC GCCGCAGAGC AGAGTGGCCT CATCCAGGCC
    GGAGACATCA TTCTTGCGGT CAACGGCCGG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC
    CTGGAGGTAC TCAGAGGCAT TGCCTCCGAG ACCCACGTGG TCCTCATTCT GAGGGGTCCT
    GAAGGTTTCA CCACGCACCT GGAGACCACC TTTACAGGTG ATGGGACCCC CAAGACCATC
    CGGGTGACAC AGCCCCTGGG TCCCCCCACC AAAGCCGTGG ATCTGTCCCA CCAGCCATCG
    GCCGGCAAAG AACAGCCGCT GGCAGTGGAT GGGGCCTCGG GTCCCGGGAA TGGGCCTCAG
    CATGCCTACG ATGACGGGCA GGAGGCTGGC TCACTCCCCC ATGCCAACGG CCTGGCCCCC
    AGGCCCCCAG GCCAGGACCC TGCGAAGAAA GCAACCAGAG TCAGCCTCCA AGGCAGAGGG
    GAGAACAATG AACTGCTCAA GGAGATAGAG CCTGTGCTGA GCCTTCTCAC CAGTGGGAGC
    AGAGGGGTCA AGGGAGGGGC ACCTGCCAAG GCAGAGATGA AAGATATGGG AATCCAGGTG
    GACAGAGATT TGGACGGCAA GTCACACAAA CCTCTGCCCC TCGCCGTGGA GAATGACCGA
    GTCTTCAATG ACCTATGGGG GAAGGGCAAT GTGCCTGTCG TCCTCAACAA CCCATATTCA
    GAGAAGGAGC AGCCCCCTGC CTCAGGAAAA CAGTCCCCCA CAAAGAATGG CAGCCCCTCC
    AAGTGTCCAC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG AGGTGGTTCT CACTGACACC
    CTCCACCTTA AGAGCACATT GGAAACGGGA TGCACTGAGT ACATCTGCAT GGGCTCCATC
    ATGCATCCTT CTCAGCATGC AAGGAGGCCT GAAGATGTCC GCACAAAAGA ACAGCTCTTC
    CCTCTCGCCA AAGAGTTTAT TGATCAATAC TATTCATCAA TTAAAAGATT TGGCTCCAAA
    GCCCACATGG AAAGGCTGGA AGAGGTGAAC AAAGAGATCG AAACCACTAG CACCTACCAG
    CTCAAGGACA CAGAGCTCAT CTATGGGGCC AAGCACGCCT GGCGGAATGC CTCGCGCTGT
    GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTATTCGATG CCCGTGACTG CACCACGGCC
    CACGGGATGT TCAACTACAT CTGTAACCAC GTCAAGTATG CCACCAACAA AGGGAACCTC
    AGGTCCGCCA TCACCATATT CCCCCAGAGG ACAGACGGCA AGCACGACTT CCGAGTCTGG
    AACTCCCAGC TCATCCGCTA CGCTGGCTAC AAGCAGCCCG ACGGCTCCAC CCTGGGGGAC
    CCAGCCAATG TGCAGTTCAC AGAGATATGC ATACAGCAGG GCTGGAAACC GCCTAGAGGC
    CGCTTCGATG TCCTGCCGCT CCTGCTTCAG GCCAACGGCA ATGACCCTGA GCTCTTCCAG
    ATTCCTCCAG AGCTGGTGTT GGAAGTTCCC ATCAGGCACC CCAAGTTTGA GTGGTTCAAG
    GACCTGGGGC TGAAGTGGTA CGGCCTCCCC GCCGTGTCCA ACATGCTCCT AGAGATTGGC
    GGCCTGGAGT TCAGCGCCTG TCCCTTCAGT GGCTGGTACA TGGGCACAGA GATTGGTGTC
    CGCGACTACT GTGACAACTC CCGCTACAAT ATCCTGGAGG AAGTGGCCAA GAAGATGAAC
    TTAGACATGA GGAAGATGTC CTCCCTGTGG AAGGACCAGG CGCTGGTGGA GATCAATATC
    GCGGTTCTCT ACAGCTTCCA GAGTGACAAA GTGACCATTG TTGACCATCA CTCCGCCACC
    GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGGGGCTG CCCTGCCGAC
    TGGGTGTGGA TCGTGCCCCC CATGTCCGGA AGCATCACCC CTGTGTTCCA CCAGGAGATG
    CTCAACTACC GGCTCACCCC CTCCTTCGAA TACCAGCCTG ATCCCTGGAA CACGCATGTC
    TGGAAAGGCA CCAACGGGAC CCCCACAAAG CGGCGAGCCA TCGGCTTCAA GAAGCTAGCA
    GAAGCTGTCA AGTTCTCGGC CAAGCTGATG GGGCAGGCTA TGGCCAAGAG GGTCAAAGCG
    ACCATCCTCT ATGCCACAGA GACAGGCAAA TCGCAAGCTT ATGCCAAGAC CTTGTGTGAG
    ATCTTCAAAC ACGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATCGTGCAC
    CTGGAACATG AAACTCTGGT CCTCGTGGTC ACCAGCACCT TTGGCAATGG AGATCCCCCT
    GAGAATGGGG AGAAATTCGG CTGTGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAG
    GAAGAAAGGA AGTACCCGGA ACCCTTGCGT TTCTTTCCCC GTAAAGGGCC TCCCCTCCCC
    AATGGTGACA CAGAAGTCCA CGGTCTGGCT GCAGCCCGTG ACAGCCAGCA CAGGAGCTAC
    AAGGTCCGAT TCAACAGCGT CTCCTCCTAC TCTGACTCCC AAAAATCATC AGGCGATGGG
    CCCGACCTCA GAGACAACTT TGAGAGTGCT GGACCCCTGG CCAATGTGAG GTTCTCAGTT
    TTTGGCCTCG GCTCACGAGC ATACCCTCAC TTTTGTGCCT TCGGACACGC TGTGGACACC
    CTCCTGGAAG AACTGGGAGG GGAGAGGATC CTGAAGATGA GGGAAGGGGA TGAGCTCTGT
    GGGCAGGAAG AGGCTTTCAG GACCTGGGCC AAGAAGGTCT TCAAGGCAGC CTGTGATGTC
    TTCTGTGTGG GAGATGATGT CAACATTGAA AAGGCCAACA ATTCCCTCAT CAGCAATGAT
    CGCAGCTGGA AGAGAAACAA GTTCCGCCTC ACCTTTGTGG CCGAAGCTCC AGAACTCACA
    CAAGGTCTAT CCAATGTCCA CAAAAAGCGA GTCTCAGCTG CCCGGCTCCT TAGCCGTCAA
    AACCTCCAGA GTCCTAAATC CAGTCGGTCA ACTATCTTCG TGCGTCTCCA CACCAACGGG
    AACCAGGAGC TGCAGTACCA GCCTGGGGAC CACCTGGGTG TCTTCCCTGG CAACCACGAG
    GACCTCGTGA ATGCCCTGAT CGAGCGGCTG GAGGACGCGC CCCCTGTCAA CCAGATGGTG
    AAAGTGGAAC TGCTGGAGGA GCGGAACACG GCTTTAGGTG TCATCAGTAA CTGGACAGAC
    GAGCTCCGCC TCCCGCCCTG CACCATCTTC CAGGCCTTCA AGTACTACCT GGACATCACC
    ACGCCACCAA CGCCCCTGCA GCTGCAGCAG TTTGCCTCCC TAGCTACCAG CGAGAAGGAG
    AAGCAGCGTC TGCTGGTCCT CAGCAAGGGT TTGCAGGAGT ACGAGGAATG GAAATGGGGC
    AAGAACCCCA CCATCGTGGA GGTGCTGGAG GAATTCCCAT CTATCCAGAT GCCGGCCACC
    CTGCTCCTGA CCCAGCTGTC CCTGCTGCAG CCCCGCTACT ATTCCATCAG CTCCTCCCCA
    GACATGTACC CTGATGAAGT GCACCTCACT GTGGCCATCG TTTCCTACCG CACTCGAGAT
    GGAGAAGGAC CAATTCACCA CGGCGTATGC TCCTCCTGGC TCAACCGGAT ACAGGCTGAC
    GAACTGGTCC CCTGTTTCGT GAGAGGAGCA CCCAGCTTCC ACCTGCCCCG GAACCCCCAA
    GTCCCCTGCA TCCTCGTTGG ACCAGGCACC GGCATTGCCC CTTTCCGAAG CTTCTGGCAA
    CAGCGGCAAT TTGATATCCA ACACAAAGGA ATGAACCCCT GCCCCATGGT CCTGGTCTTC
    GGGTGCCGGC AATCCAAGAT AGATCATATC TACAGGGAAG AGACCCTGCA GGCCAAGAAC
    AAGGGGGTCT TCAGAGAGCT GTACACGGCT TACTCCCGGG AGCCAGACAA ACCAAAGAAG
    TATGTGCAGG ACATCCTGCA GGAGCAGCTG GCGGAGTCTG TGTACCGAGC CCTGAAGGAG
    CAAGGGGGCC ACATATACGT CTGTGGGGAC GTCACCATGG CTGCTGATGT CCTCAAAGCC
    ATCCAGCGCA TCATGACCCA GCAGGGGAAG CTCTCGGCAG AGGACGCCGG CGTATTCATC
    AGCCGGATGA GGGATGACAA CCGATACCAT GAGGATATTT TTGGAGTCAC CCTGCGAACG
    TACGAAGTGA CCAACCGCCT TAGATCTGAG TCCATTGCCT TCATTGAAGA GAGCAAAAAA
    GACACCGATG AGGTTTTCAG CTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc66615
    Clone ID Related Accession (Same CDS sequence) XM_016924331.1 , XM_016924327.2 , XM_016924331.2 , XM_016924328.1 , XM_016924332.2 , XM_016924327.1 , XM_016924332.1 , XM_016924328.2 , XM_016924329.2 , XM_016924329.1
    Accession Version XM_016924329.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4407bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-01
    Organism Pan troglodytes(chimpanzee)
    Product nitric oxide synthase, brain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006479.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAGGATC ACATGTTCGG TGTTCAGCAA ATCCAGCCCA ATGTCATTTC TGTTCGTCTC 
    TTCAAGCGCA AAGTTGGGGG CCTGGGATTC CTGGTGAAGG AGCGGGTCAG TAAGCCGCCC
    GTGATTATCT CTGACCTGAT TCGTGGGGGC GCCGCAGAGC AGAGTGGCCT CATCCAGGCC
    GGAGACATCA TTCTTGCGGT CAACGGCCGG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC
    CTGGAGGTAC TCAGAGGCAT TGCCTCCGAG ACCCACGTGG TCCTCATTCT GAGGGGTCCT
    GAAGGTTTCA CCACGCACCT GGAGACCACC TTTACAGGTG ATGGGACCCC CAAGACCATC
    CGGGTGACAC AGCCCCTGGG TCCCCCCACC AAAGCCGTGG ATCTGTCCCA CCAGCCATCG
    GCCGGCAAAG AACAGCCGCT GGCAGTGGAT GGGGCCTCGG GTCCCGGGAA TGGGCCTCAG
    CATGCCTACG ATGACGGGCA GGAGGCTGGC TCACTCCCCC ATGCCAACGG CCTGGCCCCC
    AGGCCCCCAG GCCAGGACCC TGCGAAGAAA GCAACCAGAG TCAGCCTCCA AGGCAGAGGG
    GAGAACAATG AACTGCTCAA GGAGATAGAG CCTGTGCTGA GCCTTCTCAC CAGTGGGAGC
    AGAGGGGTCA AGGGAGGGGC ACCTGCCAAG GCAGAGATGA AAGATATGGG AATCCAGGTG
    GACAGAGATT TGGACGGCAA GTCACACAAA CCTCTGCCCC TCGCCGTGGA GAATGACCGA
    GTCTTCAATG ACCTATGGGG GAAGGGCAAT GTGCCTGTCG TCCTCAACAA CCCATATTCA
    GAGAAGGAGC AGCCCCCTGC CTCAGGAAAA CAGTCCCCCA CAAAGAATGG CAGCCCCTCC
    AAGTGTCCAC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG AGGTGGTTCT CACTGACACC
    CTCCACCTTA AGAGCACATT GGAAACGGGA TGCACTGAGT ACATCTGCAT GGGCTCCATC
    ATGCATCCTT CTCAGCATGC AAGGAGGCCT GAAGATGTCC GCACAAAAGA ACAGCTCTTC
    CCTCTCGCCA AAGAGTTTAT TGATCAATAC TATTCATCAA TTAAAAGATT TGGCTCCAAA
    GCCCACATGG AAAGGCTGGA AGAGGTGAAC AAAGAGATCG AAACCACTAG CACCTACCAG
    CTCAAGGACA CAGAGCTCAT CTATGGGGCC AAGCACGCCT GGCGGAATGC CTCGCGCTGT
    GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTATTCGATG CCCGTGACTG CACCACGGCC
    CACGGGATGT TCAACTACAT CTGTAACCAC GTCAAGTATG CCACCAACAA AGGGAACCTC
    AGGTCCGCCA TCACCATATT CCCCCAGAGG ACAGACGGCA AGCACGACTT CCGAGTCTGG
    AACTCCCAGC TCATCCGCTA CGCTGGCTAC AAGCAGCCCG ACGGCTCCAC CCTGGGGGAC
    CCAGCCAATG TGCAGTTCAC AGAGATATGC ATACAGCAGG GCTGGAAACC GCCTAGAGGC
    CGCTTCGATG TCCTGCCGCT CCTGCTTCAG GCCAACGGCA ATGACCCTGA GCTCTTCCAG
    ATTCCTCCAG AGCTGGTGTT GGAAGTTCCC ATCAGGCACC CCAAGTTTGA GTGGTTCAAG
    GACCTGGGGC TGAAGTGGTA CGGCCTCCCC GCCGTGTCCA ACATGCTCCT AGAGATTGGC
    GGCCTGGAGT TCAGCGCCTG TCCCTTCAGT GGCTGGTACA TGGGCACAGA GATTGGTGTC
    CGCGACTACT GTGACAACTC CCGCTACAAT ATCCTGGAGG AAGTGGCCAA GAAGATGAAC
    TTAGACATGA GGAAGATGTC CTCCCTGTGG AAGGACCAGG CGCTGGTGGA GATCAATATC
    GCGGTTCTCT ACAGCTTCCA GAGTGACAAA GTGACCATTG TTGACCATCA CTCCGCCACC
    GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGGGGCTG CCCTGCCGAC
    TGGGTGTGGA TCGTGCCCCC CATGTCCGGA AGCATCACCC CTGTGTTCCA CCAGGAGATG
    CTCAACTACC GGCTCACCCC CTCCTTCGAA TACCAGCCTG ATCCCTGGAA CACGCATGTC
    TGGAAAGGCA CCAACGGGAC CCCCACAAAG CGGCGAGCCA TCGGCTTCAA GAAGCTAGCA
    GAAGCTGTCA AGTTCTCGGC CAAGCTGATG GGGCAGGCTA TGGCCAAGAG GGTCAAAGCG
    ACCATCCTCT ATGCCACAGA GACAGGCAAA TCGCAAGCTT ATGCCAAGAC CTTGTGTGAG
    ATCTTCAAAC ACGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATCGTGCAC
    CTGGAACATG AAACTCTGGT CCTCGTGGTC ACCAGCACCT TTGGCAATGG AGATCCCCCT
    GAGAATGGGG AGAAATTCGG CTGTGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAG
    GAAGAAAGGA AGTACCCGGA ACCCTTGCGT TTCTTTCCCC GTAAAGGGCC TCCCCTCCCC
    AATGGTGACA CAGAAGTCCA CGGTCTGGCT GCAGCCCGTG ACAGCCAGCA CAGGAGCTAC
    AAGGTCCGAT TCAACAGCGT CTCCTCCTAC TCTGACTCCC AAAAATCATC AGGCGATGGG
    CCCGACCTCA GAGACAACTT TGAGAGTGCT GGACCCCTGG CCAATGTGAG GTTCTCAGTT
    TTTGGCCTCG GCTCACGAGC ATACCCTCAC TTTTGTGCCT TCGGACACGC TGTGGACACC
    CTCCTGGAAG AACTGGGAGG GGAGAGGATC CTGAAGATGA GGGAAGGGGA TGAGCTCTGT
    GGGCAGGAAG AGGCTTTCAG GACCTGGGCC AAGAAGGTCT TCAAGGCAGC CTGTGATGTC
    TTCTGTGTGG GAGATGATGT CAACATTGAA AAGGCCAACA ATTCCCTCAT CAGCAATGAT
    CGCAGCTGGA AGAGAAACAA GTTCCGCCTC ACCTTTGTGG CCGAAGCTCC AGAACTCACA
    CAAGGTCTAT CCAATGTCCA CAAAAAGCGA GTCTCAGCTG CCCGGCTCCT TAGCCGTCAA
    AACCTCCAGA GTCCTAAATC CAGTCGGTCA ACTATCTTCG TGCGTCTCCA CACCAACGGG
    AACCAGGAGC TGCAGTACCA GCCTGGGGAC CACCTGGGTG TCTTCCCTGG CAACCACGAG
    GACCTCGTGA ATGCCCTGAT CGAGCGGCTG GAGGACGCGC CCCCTGTCAA CCAGATGGTG
    AAAGTGGAAC TGCTGGAGGA GCGGAACACG GCTTTAGGTG TCATCAGTAA CTGGACAGAC
    GAGCTCCGCC TCCCGCCCTG CACCATCTTC CAGGCCTTCA AGTACTACCT GGACATCACC
    ACGCCACCAA CGCCCCTGCA GCTGCAGCAG TTTGCCTCCC TAGCTACCAG CGAGAAGGAG
    AAGCAGCGTC TGCTGGTCCT CAGCAAGGGT TTGCAGGAGT ACGAGGAATG GAAATGGGGC
    AAGAACCCCA CCATCGTGGA GGTGCTGGAG GAATTCCCAT CTATCCAGAT GCCGGCCACC
    CTGCTCCTGA CCCAGCTGTC CCTGCTGCAG CCCCGCTACT ATTCCATCAG CTCCTCCCCA
    GACATGTACC CTGATGAAGT GCACCTCACT GTGGCCATCG TTTCCTACCG CACTCGAGAT
    GGAGAAGGAC CAATTCACCA CGGCGTATGC TCCTCCTGGC TCAACCGGAT ACAGGCTGAC
    GAACTGGTCC CCTGTTTCGT GAGAGGAGCA CCCAGCTTCC ACCTGCCCCG GAACCCCCAA
    GTCCCCTGCA TCCTCGTTGG ACCAGGCACC GGCATTGCCC CTTTCCGAAG CTTCTGGCAA
    CAGCGGCAAT TTGATATCCA ACACAAAGGA ATGAACCCCT GCCCCATGGT CCTGGTCTTC
    GGGTGCCGGC AATCCAAGAT AGATCATATC TACAGGGAAG AGACCCTGCA GGCCAAGAAC
    AAGGGGGTCT TCAGAGAGCT GTACACGGCT TACTCCCGGG AGCCAGACAA ACCAAAGAAG
    TATGTGCAGG ACATCCTGCA GGAGCAGCTG GCGGAGTCTG TGTACCGAGC CCTGAAGGAG
    CAAGGGGGCC ACATATACGT CTGTGGGGAC GTCACCATGG CTGCTGATGT CCTCAAAGCC
    ATCCAGCGCA TCATGACCCA GCAGGGGAAG CTCTCGGCAG AGGACGCCGG CGTATTCATC
    AGCCGGATGA GGGATGACAA CCGATACCAT GAGGATATTT TTGGAGTCAC CCTGCGAACG
    TACGAAGTGA CCAACCGCCT TAGATCTGAG TCCATTGCCT TCATTGAAGA GAGCAAAAAA
    GACACCGATG AGGTTTTCAG CTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016779818.1
    CDS2124..6530
    Translation

    Target ORF information:

    RefSeq Version XM_016924329.1
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes nitric oxide synthase 1 (NOS1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_016924329.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAGGATC ACATGTTCGG TGTTCAGCAA ATCCAGCCCA ATGTCATTTC TGTTCGTCTC 
    TTCAAGCGCA AAGTTGGGGG CCTGGGATTC CTGGTGAAGG AGCGGGTCAG TAAGCCGCCC
    GTGATTATCT CTGACCTGAT TCGTGGGGGC GCCGCAGAGC AGAGTGGCCT CATCCAGGCC
    GGAGACATCA TTCTTGCGGT CAACGGCCGG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC
    CTGGAGGTAC TCAGAGGCAT TGCCTCCGAG ACCCACGTGG TCCTCATTCT GAGGGGTCCT
    GAAGGTTTCA CCACGCACCT GGAGACCACC TTTACAGGTG ATGGGACCCC CAAGACCATC
    CGGGTGACAC AGCCCCTGGG TCCCCCCACC AAAGCCGTGG ATCTGTCCCA CCAGCCATCG
    GCCGGCAAAG AACAGCCGCT GGCAGTGGAT GGGGCCTCGG GTCCCGGGAA TGGGCCTCAG
    CATGCCTACG ATGACGGGCA GGAGGCTGGC TCACTCCCCC ATGCCAACGG CCTGGCCCCC
    AGGCCCCCAG GCCAGGACCC TGCGAAGAAA GCAACCAGAG TCAGCCTCCA AGGCAGAGGG
    GAGAACAATG AACTGCTCAA GGAGATAGAG CCTGTGCTGA GCCTTCTCAC CAGTGGGAGC
    AGAGGGGTCA AGGGAGGGGC ACCTGCCAAG GCAGAGATGA AAGATATGGG AATCCAGGTG
    GACAGAGATT TGGACGGCAA GTCACACAAA CCTCTGCCCC TCGCCGTGGA GAATGACCGA
    GTCTTCAATG ACCTATGGGG GAAGGGCAAT GTGCCTGTCG TCCTCAACAA CCCATATTCA
    GAGAAGGAGC AGCCCCCTGC CTCAGGAAAA CAGTCCCCCA CAAAGAATGG CAGCCCCTCC
    AAGTGTCCAC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG AGGTGGTTCT CACTGACACC
    CTCCACCTTA AGAGCACATT GGAAACGGGA TGCACTGAGT ACATCTGCAT GGGCTCCATC
    ATGCATCCTT CTCAGCATGC AAGGAGGCCT GAAGATGTCC GCACAAAAGA ACAGCTCTTC
    CCTCTCGCCA AAGAGTTTAT TGATCAATAC TATTCATCAA TTAAAAGATT TGGCTCCAAA
    GCCCACATGG AAAGGCTGGA AGAGGTGAAC AAAGAGATCG AAACCACTAG CACCTACCAG
    CTCAAGGACA CAGAGCTCAT CTATGGGGCC AAGCACGCCT GGCGGAATGC CTCGCGCTGT
    GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTATTCGATG CCCGTGACTG CACCACGGCC
    CACGGGATGT TCAACTACAT CTGTAACCAC GTCAAGTATG CCACCAACAA AGGGAACCTC
    AGGTCCGCCA TCACCATATT CCCCCAGAGG ACAGACGGCA AGCACGACTT CCGAGTCTGG
    AACTCCCAGC TCATCCGCTA CGCTGGCTAC AAGCAGCCCG ACGGCTCCAC CCTGGGGGAC
    CCAGCCAATG TGCAGTTCAC AGAGATATGC ATACAGCAGG GCTGGAAACC GCCTAGAGGC
    CGCTTCGATG TCCTGCCGCT CCTGCTTCAG GCCAACGGCA ATGACCCTGA GCTCTTCCAG
    ATTCCTCCAG AGCTGGTGTT GGAAGTTCCC ATCAGGCACC CCAAGTTTGA GTGGTTCAAG
    GACCTGGGGC TGAAGTGGTA CGGCCTCCCC GCCGTGTCCA ACATGCTCCT AGAGATTGGC
    GGCCTGGAGT TCAGCGCCTG TCCCTTCAGT GGCTGGTACA TGGGCACAGA GATTGGTGTC
    CGCGACTACT GTGACAACTC CCGCTACAAT ATCCTGGAGG AAGTGGCCAA GAAGATGAAC
    TTAGACATGA GGAAGATGTC CTCCCTGTGG AAGGACCAGG CGCTGGTGGA GATCAATATC
    GCGGTTCTCT ACAGCTTCCA GAGTGACAAA GTGACCATTG TTGACCATCA CTCCGCCACC
    GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGGGGCTG CCCTGCCGAC
    TGGGTGTGGA TCGTGCCCCC CATGTCCGGA AGCATCACCC CTGTGTTCCA CCAGGAGATG
    CTCAACTACC GGCTCACCCC CTCCTTCGAA TACCAGCCTG ATCCCTGGAA CACGCATGTC
    TGGAAAGGCA CCAACGGGAC CCCCACAAAG CGGCGAGCCA TCGGCTTCAA GAAGCTAGCA
    GAAGCTGTCA AGTTCTCGGC CAAGCTGATG GGGCAGGCTA TGGCCAAGAG GGTCAAAGCG
    ACCATCCTCT ATGCCACAGA GACAGGCAAA TCGCAAGCTT ATGCCAAGAC CTTGTGTGAG
    ATCTTCAAAC ACGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATCGTGCAC
    CTGGAACATG AAACTCTGGT CCTCGTGGTC ACCAGCACCT TTGGCAATGG AGATCCCCCT
    GAGAATGGGG AGAAATTCGG CTGTGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAG
    GAAGAAAGGA AGTACCCGGA ACCCTTGCGT TTCTTTCCCC GTAAAGGGCC TCCCCTCCCC
    AATGGTGACA CAGAAGTCCA CGGTCTGGCT GCAGCCCGTG ACAGCCAGCA CAGGAGCTAC
    AAGGTCCGAT TCAACAGCGT CTCCTCCTAC TCTGACTCCC AAAAATCATC AGGCGATGGG
    CCCGACCTCA GAGACAACTT TGAGAGTGCT GGACCCCTGG CCAATGTGAG GTTCTCAGTT
    TTTGGCCTCG GCTCACGAGC ATACCCTCAC TTTTGTGCCT TCGGACACGC TGTGGACACC
    CTCCTGGAAG AACTGGGAGG GGAGAGGATC CTGAAGATGA GGGAAGGGGA TGAGCTCTGT
    GGGCAGGAAG AGGCTTTCAG GACCTGGGCC AAGAAGGTCT TCAAGGCAGC CTGTGATGTC
    TTCTGTGTGG GAGATGATGT CAACATTGAA AAGGCCAACA ATTCCCTCAT CAGCAATGAT
    CGCAGCTGGA AGAGAAACAA GTTCCGCCTC ACCTTTGTGG CCGAAGCTCC AGAACTCACA
    CAAGGTCTAT CCAATGTCCA CAAAAAGCGA GTCTCAGCTG CCCGGCTCCT TAGCCGTCAA
    AACCTCCAGA GTCCTAAATC CAGTCGGTCA ACTATCTTCG TGCGTCTCCA CACCAACGGG
    AACCAGGAGC TGCAGTACCA GCCTGGGGAC CACCTGGGTG TCTTCCCTGG CAACCACGAG
    GACCTCGTGA ATGCCCTGAT CGAGCGGCTG GAGGACGCGC CCCCTGTCAA CCAGATGGTG
    AAAGTGGAAC TGCTGGAGGA GCGGAACACG GCTTTAGGTG TCATCAGTAA CTGGACAGAC
    GAGCTCCGCC TCCCGCCCTG CACCATCTTC CAGGCCTTCA AGTACTACCT GGACATCACC
    ACGCCACCAA CGCCCCTGCA GCTGCAGCAG TTTGCCTCCC TAGCTACCAG CGAGAAGGAG
    AAGCAGCGTC TGCTGGTCCT CAGCAAGGGT TTGCAGGAGT ACGAGGAATG GAAATGGGGC
    AAGAACCCCA CCATCGTGGA GGTGCTGGAG GAATTCCCAT CTATCCAGAT GCCGGCCACC
    CTGCTCCTGA CCCAGCTGTC CCTGCTGCAG CCCCGCTACT ATTCCATCAG CTCCTCCCCA
    GACATGTACC CTGATGAAGT GCACCTCACT GTGGCCATCG TTTCCTACCG CACTCGAGAT
    GGAGAAGGAC CAATTCACCA CGGCGTATGC TCCTCCTGGC TCAACCGGAT ACAGGCTGAC
    GAACTGGTCC CCTGTTTCGT GAGAGGAGCA CCCAGCTTCC ACCTGCCCCG GAACCCCCAA
    GTCCCCTGCA TCCTCGTTGG ACCAGGCACC GGCATTGCCC CTTTCCGAAG CTTCTGGCAA
    CAGCGGCAAT TTGATATCCA ACACAAAGGA ATGAACCCCT GCCCCATGGT CCTGGTCTTC
    GGGTGCCGGC AATCCAAGAT AGATCATATC TACAGGGAAG AGACCCTGCA GGCCAAGAAC
    AAGGGGGTCT TCAGAGAGCT GTACACGGCT TACTCCCGGG AGCCAGACAA ACCAAAGAAG
    TATGTGCAGG ACATCCTGCA GGAGCAGCTG GCGGAGTCTG TGTACCGAGC CCTGAAGGAG
    CAAGGGGGCC ACATATACGT CTGTGGGGAC GTCACCATGG CTGCTGATGT CCTCAAAGCC
    ATCCAGCGCA TCATGACCCA GCAGGGGAAG CTCTCGGCAG AGGACGCCGG CGTATTCATC
    AGCCGGATGA GGGATGACAA CCGATACCAT GAGGATATTT TTGGAGTCAC CCTGCGAACG
    TACGAAGTGA CCAACCGCCT TAGATCTGAG TCCATTGCCT TCATTGAAGA GAGCAAAAAA
    GACACCGATG AGGTTTTCAG CTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc66616
    Clone ID Related Accession (Same CDS sequence) XM_009426346.3 , XM_009426346.2
    Accession Version XM_009426346.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4305bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-19
    Organism Pan troglodytes(chimpanzee)
    Product nitric oxide synthase, brain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036891.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009426346.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAGGATC ACATGTTCGG TGTTCAGCAA ATCCAGCCCA ATGTCATTTC TGTTCGTCTC 
    TTCAAGCGCA AAGTTGGGGG CCTGGGATTC CTGGTGAAGG AGCGGGTCAG TAAGCCGCCC
    GTGATTATCT CTGACCTGAT TCGTGGGGGC GCCGCAGAGC AGAGTGGCCT CATCCAGGCC
    GGAGACATCA TTCTTGCGGT CAACGGCCGG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC
    CTGGAGGTAC TCAGAGGCAT TGCCTCCGAG ACCCACGTGG TCCTCATTCT GAGGGGTCCT
    GAAGGTTTCA CCACGCACCT GGAGACCACC TTTACAGGTG ATGGGACCCC CAAGACCATC
    CGGGTGACAC AGCCCCTGGG TCCCCCCACC AAAGCCGTGG ATCTGTCCCA CCAGCCATCG
    GCCGGCAAAG AACAGCCGCT GGCAGTGGAT GGGGCCTCGG GTCCCGGGAA TGGGCCTCAG
    CATGCCTACG ATGACGGGCA GGAGGCTGGC TCACTCCCCC ATGCCAACGG CCTGGCCCCC
    AGGCCCCCAG GCCAGGACCC TGCGAAGAAA GCAACCAGAG TCAGCCTCCA AGGCAGAGGG
    GAGAACAATG AACTGCTCAA GGAGATAGAG CCTGTGCTGA GCCTTCTCAC CAGTGGGAGC
    AGAGGGGTCA AGGGAGGGGC ACCTGCCAAG GCAGAGATGA AAGATATGGG AATCCAGGTG
    GACAGAGATT TGGACGGCAA GTCACACAAA CCTCTGCCCC TCGCCGTGGA GAATGACCGA
    GTCTTCAATG ACCTATGGGG GAAGGGCAAT GTGCCTGTCG TCCTCAACAA CCCATATTCA
    GAGAAGGAGC AGCCCCCTGC CTCAGGAAAA CAGTCCCCCA CAAAGAATGG CAGCCCCTCC
    AAGTGTCCAC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG AGGTGGTTCT CACTGACACC
    CTCCACCTTA AGAGCACATT GGAAACGGGA TGCACTGAGT ACATCTGCAT GGGCTCCATC
    ATGCATCCTT CTCAGCATGC AAGGAGGCCT GAAGATGTCC GCACAAAAGA ACAGCTCTTC
    CCTCTCGCCA AAGAGTTTAT TGATCAATAC TATTCATCAA TTAAAAGATT TGGCTCCAAA
    GCCCACATGG AAAGGCTGGA AGAGGTGAAC AAAGAGATCG AAACCACTAG CACCTACCAG
    CTCAAGGACA CAGAGCTCAT CTATGGGGCC AAGCACGCCT GGCGGAATGC CTCGCGCTGT
    GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTATTCGATG CCCGTGACTG CACCACGGCC
    CACGGGATGT TCAACTACAT CTGTAACCAC GTCAAGTATG CCACCAACAA AGGGAACCTC
    AGGTCCGCCA TCACCATATT CCCCCAGAGG ACAGACGGCA AGCACGACTT CCGAGTCTGG
    AACTCCCAGC TCATCCGCTA CGCTGGCTAC AAGCAGCCCG ACGGCTCCAC CCTGGGGGAC
    CCAGCCAATG TGCAGTTCAC AGAGATATGC ATACAGCAGG GCTGGAAACC GCCTAGAGGC
    CGCTTCGATG TCCTGCCGCT CCTGCTTCAG GCCAACGGCA ATGACCCTGA GCTCTTCCAG
    ATTCCTCCAG AGCTGGTGTT GGAAGTTCCC ATCAGGCACC CCAAGTTTGA GTGGTTCAAG
    GACCTGGGGC TGAAGTGGTA CGGCCTCCCC GCCGTGTCCA ACATGCTCCT AGAGATTGGC
    GGCCTGGAGT TCAGCGCCTG TCCCTTCAGT GGCTGGTACA TGGGCACAGA GATTGGTGTC
    CGCGACTACT GTGACAACTC CCGCTACAAT ATCCTGGAGG AAGTGGCCAA GAAGATGAAC
    TTAGACATGA GGAAGATGTC CTCCCTGTGG AAGGACCAGG CGCTGGTGGA GATCAATATC
    GCGGTTCTCT ACAGCTTCCA GAGTGACAAA GTGACCATTG TTGACCATCA CTCCGCCACC
    GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGGGGCTG CCCTGCCGAC
    TGGGTGTGGA TCGTGCCCCC CATGTCCGGA AGCATCACCC CTGTGTTCCA CCAGGAGATG
    CTCAACTACC GGCTCACCCC CTCCTTCGAA TACCAGCCTG ATCCCTGGAA CACGCATGTC
    TGGAAAGGCA CCAACGGGAC CCCCACAAAG CGGCGAGCCA TCGGCTTCAA GAAGCTAGCA
    GAAGCTGTCA AGTTCTCGGC CAAGCTGATG GGGCAGGCTA TGGCCAAGAG GGTCAAAGCG
    ACCATCCTCT ATGCCACAGA GACAGGCAAA TCGCAAGCTT ATGCCAAGAC CTTGTGTGAG
    ATCTTCAAAC ACGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATCGTGCAC
    CTGGAACATG AAACTCTGGT CCTCGTGGTC ACCAGCACCT TTGGCAATGG AGATCCCCCT
    GAGAATGGGG AGAAATTCGG CTGTGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAG
    GAAGAAAGGA AGAGCTACAA GGTCCGATTC AACAGCGTCT CCTCCTACTC TGACTCCCAA
    AAATCATCAG GCGATGGGCC CGACCTCAGA GACAACTTTG AGAGTGCTGG ACCCCTGGCC
    AATGTGAGGT TCTCAGTTTT TGGCCTCGGC TCACGAGCAT ACCCTCACTT TTGTGCCTTC
    GGACACGCTG TGGACACCCT CCTGGAAGAA CTGGGAGGGG AGAGGATCCT GAAGATGAGG
    GAAGGGGATG AGCTCTGTGG GCAGGAAGAG GCTTTCAGGA CCTGGGCCAA GAAGGTCTTC
    AAGGCAGCCT GTGATGTCTT CTGTGTGGGA GATGATGTCA ACATTGAAAA GGCCAACAAT
    TCCCTCATCA GCAATGATCG CAGCTGGAAG AGAAACAAGT TCCGCCTCAC CTTTGTGGCC
    GAAGCTCCAG AACTCACACA AGGTCTATCC AATGTCCACA AAAAGCGAGT CTCAGCTGCC
    CGGCTCCTTA GCCGTCAAAA CCTCCAGAGT CCTAAATCCA GTCGGTCAAC TATCTTCGTG
    CGTCTCCACA CCAACGGGAA CCAGGAGCTG CAGTACCAGC CTGGGGACCA CCTGGGTGTC
    TTCCCTGGCA ACCACGAGGA CCTCGTGAAT GCCCTGATCG AGCGGCTGGA GGACGCGCCC
    CCTGTCAACC AGATGGTGAA AGTGGAACTG CTGGAGGAGC GGAACACGGC TTTAGGTGTC
    ATCAGTAACT GGACAGACGA GCTCCGCCTC CCGCCCTGCA CCATCTTCCA GGCCTTCAAG
    TACTACCTGG ACATCACCAC GCCACCAACG CCCCTGCAGC TGCAGCAGTT TGCCTCCCTA
    GCTACCAGCG AGAAGGAGAA GCAGCGTCTG CTGGTCCTCA GCAAGGGTTT GCAGGAGTAC
    GAGGAATGGA AATGGGGCAA GAACCCCACC ATCGTGGAGG TGCTGGAGGA ATTCCCATCT
    ATCCAGATGC CGGCCACCCT GCTCCTGACC CAGCTGTCCC TGCTGCAGCC CCGCTACTAT
    TCCATCAGCT CCTCCCCAGA CATGTACCCT GATGAAGTGC ACCTCACTGT GGCCATCGTT
    TCCTACCGCA CTCGAGATGG AGAAGGACCA ATTCACCACG GCGTATGCTC CTCCTGGCTC
    AACCGGATAC AGGCTGACGA ACTGGTCCCC TGTTTCGTGA GAGGAGCACC CAGCTTCCAC
    CTGCCCCGGA ACCCCCAAGT CCCCTGCATC CTCGTTGGAC CAGGCACCGG CATTGCCCCT
    TTCCGAAGCT TCTGGCAACA GCGGCAATTT GATATCCAAC ACAAAGGAAT GAACCCCTGC
    CCCATGGTCC TGGTCTTCGG GTGCCGGCAA TCCAAGATAG ATCATATCTA CAGGGAAGAG
    ACCCTGCAGG CCAAGAACAA GGGGGTCTTC AGAGAGCTGT ACACGGCTTA CTCCCGGGAG
    CCAGACAAAC CAAAGAAGTA TGTGCAGGAC ATCCTGCAGG AGCAGCTGGC GGAGTCTGTG
    TACCGAGCCC TGAAGGAGCA AGGGGGCCAC ATATACGTCT GTGGGGACGT CACCATGGCT
    GCTGATGTCC TCAAAGCCAT CCAGCGCATC ATGACCCAGC AGGGGAAGCT CTCGGCAGAG
    GACGCCGGCG TATTCATCAG CCGGATGAGG GATGACAACC GATACCATGA GGATATTTTT
    GGAGTCACCC TGCGAACGTA CGAAGTGACC AACCGCCTTA GATCTGAGTC CATTGCCTTC
    ATTGAAGAGA GCAAAAAAGA CACCGATGAG GTTTTCAGCT CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009424621.2
    CDS712..5016
    Translation

    Target ORF information:

    RefSeq Version XM_009426346.3
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes nitric oxide synthase 1 (NOS1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009426346.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAGGATC ACATGTTCGG TGTTCAGCAA ATCCAGCCCA ATGTCATTTC TGTTCGTCTC 
    TTCAAGCGCA AAGTTGGGGG CCTGGGATTC CTGGTGAAGG AGCGGGTCAG TAAGCCGCCC
    GTGATTATCT CTGACCTGAT TCGTGGGGGC GCCGCAGAGC AGAGTGGCCT CATCCAGGCC
    GGAGACATCA TTCTTGCGGT CAACGGCCGG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC
    CTGGAGGTAC TCAGAGGCAT TGCCTCCGAG ACCCACGTGG TCCTCATTCT GAGGGGTCCT
    GAAGGTTTCA CCACGCACCT GGAGACCACC TTTACAGGTG ATGGGACCCC CAAGACCATC
    CGGGTGACAC AGCCCCTGGG TCCCCCCACC AAAGCCGTGG ATCTGTCCCA CCAGCCATCG
    GCCGGCAAAG AACAGCCGCT GGCAGTGGAT GGGGCCTCGG GTCCCGGGAA TGGGCCTCAG
    CATGCCTACG ATGACGGGCA GGAGGCTGGC TCACTCCCCC ATGCCAACGG CCTGGCCCCC
    AGGCCCCCAG GCCAGGACCC TGCGAAGAAA GCAACCAGAG TCAGCCTCCA AGGCAGAGGG
    GAGAACAATG AACTGCTCAA GGAGATAGAG CCTGTGCTGA GCCTTCTCAC CAGTGGGAGC
    AGAGGGGTCA AGGGAGGGGC ACCTGCCAAG GCAGAGATGA AAGATATGGG AATCCAGGTG
    GACAGAGATT TGGACGGCAA GTCACACAAA CCTCTGCCCC TCGCCGTGGA GAATGACCGA
    GTCTTCAATG ACCTATGGGG GAAGGGCAAT GTGCCTGTCG TCCTCAACAA CCCATATTCA
    GAGAAGGAGC AGCCCCCTGC CTCAGGAAAA CAGTCCCCCA CAAAGAATGG CAGCCCCTCC
    AAGTGTCCAC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG AGGTGGTTCT CACTGACACC
    CTCCACCTTA AGAGCACATT GGAAACGGGA TGCACTGAGT ACATCTGCAT GGGCTCCATC
    ATGCATCCTT CTCAGCATGC AAGGAGGCCT GAAGATGTCC GCACAAAAGA ACAGCTCTTC
    CCTCTCGCCA AAGAGTTTAT TGATCAATAC TATTCATCAA TTAAAAGATT TGGCTCCAAA
    GCCCACATGG AAAGGCTGGA AGAGGTGAAC AAAGAGATCG AAACCACTAG CACCTACCAG
    CTCAAGGACA CAGAGCTCAT CTATGGGGCC AAGCACGCCT GGCGGAATGC CTCGCGCTGT
    GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTATTCGATG CCCGTGACTG CACCACGGCC
    CACGGGATGT TCAACTACAT CTGTAACCAC GTCAAGTATG CCACCAACAA AGGGAACCTC
    AGGTCCGCCA TCACCATATT CCCCCAGAGG ACAGACGGCA AGCACGACTT CCGAGTCTGG
    AACTCCCAGC TCATCCGCTA CGCTGGCTAC AAGCAGCCCG ACGGCTCCAC CCTGGGGGAC
    CCAGCCAATG TGCAGTTCAC AGAGATATGC ATACAGCAGG GCTGGAAACC GCCTAGAGGC
    CGCTTCGATG TCCTGCCGCT CCTGCTTCAG GCCAACGGCA ATGACCCTGA GCTCTTCCAG
    ATTCCTCCAG AGCTGGTGTT GGAAGTTCCC ATCAGGCACC CCAAGTTTGA GTGGTTCAAG
    GACCTGGGGC TGAAGTGGTA CGGCCTCCCC GCCGTGTCCA ACATGCTCCT AGAGATTGGC
    GGCCTGGAGT TCAGCGCCTG TCCCTTCAGT GGCTGGTACA TGGGCACAGA GATTGGTGTC
    CGCGACTACT GTGACAACTC CCGCTACAAT ATCCTGGAGG AAGTGGCCAA GAAGATGAAC
    TTAGACATGA GGAAGATGTC CTCCCTGTGG AAGGACCAGG CGCTGGTGGA GATCAATATC
    GCGGTTCTCT ACAGCTTCCA GAGTGACAAA GTGACCATTG TTGACCATCA CTCCGCCACC
    GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGGGGCTG CCCTGCCGAC
    TGGGTGTGGA TCGTGCCCCC CATGTCCGGA AGCATCACCC CTGTGTTCCA CCAGGAGATG
    CTCAACTACC GGCTCACCCC CTCCTTCGAA TACCAGCCTG ATCCCTGGAA CACGCATGTC
    TGGAAAGGCA CCAACGGGAC CCCCACAAAG CGGCGAGCCA TCGGCTTCAA GAAGCTAGCA
    GAAGCTGTCA AGTTCTCGGC CAAGCTGATG GGGCAGGCTA TGGCCAAGAG GGTCAAAGCG
    ACCATCCTCT ATGCCACAGA GACAGGCAAA TCGCAAGCTT ATGCCAAGAC CTTGTGTGAG
    ATCTTCAAAC ACGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATCGTGCAC
    CTGGAACATG AAACTCTGGT CCTCGTGGTC ACCAGCACCT TTGGCAATGG AGATCCCCCT
    GAGAATGGGG AGAAATTCGG CTGTGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAG
    GAAGAAAGGA AGAGCTACAA GGTCCGATTC AACAGCGTCT CCTCCTACTC TGACTCCCAA
    AAATCATCAG GCGATGGGCC CGACCTCAGA GACAACTTTG AGAGTGCTGG ACCCCTGGCC
    AATGTGAGGT TCTCAGTTTT TGGCCTCGGC TCACGAGCAT ACCCTCACTT TTGTGCCTTC
    GGACACGCTG TGGACACCCT CCTGGAAGAA CTGGGAGGGG AGAGGATCCT GAAGATGAGG
    GAAGGGGATG AGCTCTGTGG GCAGGAAGAG GCTTTCAGGA CCTGGGCCAA GAAGGTCTTC
    AAGGCAGCCT GTGATGTCTT CTGTGTGGGA GATGATGTCA ACATTGAAAA GGCCAACAAT
    TCCCTCATCA GCAATGATCG CAGCTGGAAG AGAAACAAGT TCCGCCTCAC CTTTGTGGCC
    GAAGCTCCAG AACTCACACA AGGTCTATCC AATGTCCACA AAAAGCGAGT CTCAGCTGCC
    CGGCTCCTTA GCCGTCAAAA CCTCCAGAGT CCTAAATCCA GTCGGTCAAC TATCTTCGTG
    CGTCTCCACA CCAACGGGAA CCAGGAGCTG CAGTACCAGC CTGGGGACCA CCTGGGTGTC
    TTCCCTGGCA ACCACGAGGA CCTCGTGAAT GCCCTGATCG AGCGGCTGGA GGACGCGCCC
    CCTGTCAACC AGATGGTGAA AGTGGAACTG CTGGAGGAGC GGAACACGGC TTTAGGTGTC
    ATCAGTAACT GGACAGACGA GCTCCGCCTC CCGCCCTGCA CCATCTTCCA GGCCTTCAAG
    TACTACCTGG ACATCACCAC GCCACCAACG CCCCTGCAGC TGCAGCAGTT TGCCTCCCTA
    GCTACCAGCG AGAAGGAGAA GCAGCGTCTG CTGGTCCTCA GCAAGGGTTT GCAGGAGTAC
    GAGGAATGGA AATGGGGCAA GAACCCCACC ATCGTGGAGG TGCTGGAGGA ATTCCCATCT
    ATCCAGATGC CGGCCACCCT GCTCCTGACC CAGCTGTCCC TGCTGCAGCC CCGCTACTAT
    TCCATCAGCT CCTCCCCAGA CATGTACCCT GATGAAGTGC ACCTCACTGT GGCCATCGTT
    TCCTACCGCA CTCGAGATGG AGAAGGACCA ATTCACCACG GCGTATGCTC CTCCTGGCTC
    AACCGGATAC AGGCTGACGA ACTGGTCCCC TGTTTCGTGA GAGGAGCACC CAGCTTCCAC
    CTGCCCCGGA ACCCCCAAGT CCCCTGCATC CTCGTTGGAC CAGGCACCGG CATTGCCCCT
    TTCCGAAGCT TCTGGCAACA GCGGCAATTT GATATCCAAC ACAAAGGAAT GAACCCCTGC
    CCCATGGTCC TGGTCTTCGG GTGCCGGCAA TCCAAGATAG ATCATATCTA CAGGGAAGAG
    ACCCTGCAGG CCAAGAACAA GGGGGTCTTC AGAGAGCTGT ACACGGCTTA CTCCCGGGAG
    CCAGACAAAC CAAAGAAGTA TGTGCAGGAC ATCCTGCAGG AGCAGCTGGC GGAGTCTGTG
    TACCGAGCCC TGAAGGAGCA AGGGGGCCAC ATATACGTCT GTGGGGACGT CACCATGGCT
    GCTGATGTCC TCAAAGCCAT CCAGCGCATC ATGACCCAGC AGGGGAAGCT CTCGGCAGAG
    GACGCCGGCG TATTCATCAG CCGGATGAGG GATGACAACC GATACCATGA GGATATTTTT
    GGAGTCACCC TGCGAACGTA CGAAGTGACC AACCGCCTTA GATCTGAGTC CATTGCCTTC
    ATTGAAGAGA GCAAAAAAGA CACCGATGAG GTTTTCAGCT CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc66616
    Clone ID Related Accession (Same CDS sequence) XM_009426346.3 , XM_009426346.2
    Accession Version XM_009426346.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4305bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-01
    Organism Pan troglodytes(chimpanzee)
    Product nitric oxide synthase, brain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006479.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009426346.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAGGATC ACATGTTCGG TGTTCAGCAA ATCCAGCCCA ATGTCATTTC TGTTCGTCTC 
    TTCAAGCGCA AAGTTGGGGG CCTGGGATTC CTGGTGAAGG AGCGGGTCAG TAAGCCGCCC
    GTGATTATCT CTGACCTGAT TCGTGGGGGC GCCGCAGAGC AGAGTGGCCT CATCCAGGCC
    GGAGACATCA TTCTTGCGGT CAACGGCCGG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC
    CTGGAGGTAC TCAGAGGCAT TGCCTCCGAG ACCCACGTGG TCCTCATTCT GAGGGGTCCT
    GAAGGTTTCA CCACGCACCT GGAGACCACC TTTACAGGTG ATGGGACCCC CAAGACCATC
    CGGGTGACAC AGCCCCTGGG TCCCCCCACC AAAGCCGTGG ATCTGTCCCA CCAGCCATCG
    GCCGGCAAAG AACAGCCGCT GGCAGTGGAT GGGGCCTCGG GTCCCGGGAA TGGGCCTCAG
    CATGCCTACG ATGACGGGCA GGAGGCTGGC TCACTCCCCC ATGCCAACGG CCTGGCCCCC
    AGGCCCCCAG GCCAGGACCC TGCGAAGAAA GCAACCAGAG TCAGCCTCCA AGGCAGAGGG
    GAGAACAATG AACTGCTCAA GGAGATAGAG CCTGTGCTGA GCCTTCTCAC CAGTGGGAGC
    AGAGGGGTCA AGGGAGGGGC ACCTGCCAAG GCAGAGATGA AAGATATGGG AATCCAGGTG
    GACAGAGATT TGGACGGCAA GTCACACAAA CCTCTGCCCC TCGCCGTGGA GAATGACCGA
    GTCTTCAATG ACCTATGGGG GAAGGGCAAT GTGCCTGTCG TCCTCAACAA CCCATATTCA
    GAGAAGGAGC AGCCCCCTGC CTCAGGAAAA CAGTCCCCCA CAAAGAATGG CAGCCCCTCC
    AAGTGTCCAC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG AGGTGGTTCT CACTGACACC
    CTCCACCTTA AGAGCACATT GGAAACGGGA TGCACTGAGT ACATCTGCAT GGGCTCCATC
    ATGCATCCTT CTCAGCATGC AAGGAGGCCT GAAGATGTCC GCACAAAAGA ACAGCTCTTC
    CCTCTCGCCA AAGAGTTTAT TGATCAATAC TATTCATCAA TTAAAAGATT TGGCTCCAAA
    GCCCACATGG AAAGGCTGGA AGAGGTGAAC AAAGAGATCG AAACCACTAG CACCTACCAG
    CTCAAGGACA CAGAGCTCAT CTATGGGGCC AAGCACGCCT GGCGGAATGC CTCGCGCTGT
    GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTATTCGATG CCCGTGACTG CACCACGGCC
    CACGGGATGT TCAACTACAT CTGTAACCAC GTCAAGTATG CCACCAACAA AGGGAACCTC
    AGGTCCGCCA TCACCATATT CCCCCAGAGG ACAGACGGCA AGCACGACTT CCGAGTCTGG
    AACTCCCAGC TCATCCGCTA CGCTGGCTAC AAGCAGCCCG ACGGCTCCAC CCTGGGGGAC
    CCAGCCAATG TGCAGTTCAC AGAGATATGC ATACAGCAGG GCTGGAAACC GCCTAGAGGC
    CGCTTCGATG TCCTGCCGCT CCTGCTTCAG GCCAACGGCA ATGACCCTGA GCTCTTCCAG
    ATTCCTCCAG AGCTGGTGTT GGAAGTTCCC ATCAGGCACC CCAAGTTTGA GTGGTTCAAG
    GACCTGGGGC TGAAGTGGTA CGGCCTCCCC GCCGTGTCCA ACATGCTCCT AGAGATTGGC
    GGCCTGGAGT TCAGCGCCTG TCCCTTCAGT GGCTGGTACA TGGGCACAGA GATTGGTGTC
    CGCGACTACT GTGACAACTC CCGCTACAAT ATCCTGGAGG AAGTGGCCAA GAAGATGAAC
    TTAGACATGA GGAAGATGTC CTCCCTGTGG AAGGACCAGG CGCTGGTGGA GATCAATATC
    GCGGTTCTCT ACAGCTTCCA GAGTGACAAA GTGACCATTG TTGACCATCA CTCCGCCACC
    GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGGGGCTG CCCTGCCGAC
    TGGGTGTGGA TCGTGCCCCC CATGTCCGGA AGCATCACCC CTGTGTTCCA CCAGGAGATG
    CTCAACTACC GGCTCACCCC CTCCTTCGAA TACCAGCCTG ATCCCTGGAA CACGCATGTC
    TGGAAAGGCA CCAACGGGAC CCCCACAAAG CGGCGAGCCA TCGGCTTCAA GAAGCTAGCA
    GAAGCTGTCA AGTTCTCGGC CAAGCTGATG GGGCAGGCTA TGGCCAAGAG GGTCAAAGCG
    ACCATCCTCT ATGCCACAGA GACAGGCAAA TCGCAAGCTT ATGCCAAGAC CTTGTGTGAG
    ATCTTCAAAC ACGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATCGTGCAC
    CTGGAACATG AAACTCTGGT CCTCGTGGTC ACCAGCACCT TTGGCAATGG AGATCCCCCT
    GAGAATGGGG AGAAATTCGG CTGTGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAG
    GAAGAAAGGA AGAGCTACAA GGTCCGATTC AACAGCGTCT CCTCCTACTC TGACTCCCAA
    AAATCATCAG GCGATGGGCC CGACCTCAGA GACAACTTTG AGAGTGCTGG ACCCCTGGCC
    AATGTGAGGT TCTCAGTTTT TGGCCTCGGC TCACGAGCAT ACCCTCACTT TTGTGCCTTC
    GGACACGCTG TGGACACCCT CCTGGAAGAA CTGGGAGGGG AGAGGATCCT GAAGATGAGG
    GAAGGGGATG AGCTCTGTGG GCAGGAAGAG GCTTTCAGGA CCTGGGCCAA GAAGGTCTTC
    AAGGCAGCCT GTGATGTCTT CTGTGTGGGA GATGATGTCA ACATTGAAAA GGCCAACAAT
    TCCCTCATCA GCAATGATCG CAGCTGGAAG AGAAACAAGT TCCGCCTCAC CTTTGTGGCC
    GAAGCTCCAG AACTCACACA AGGTCTATCC AATGTCCACA AAAAGCGAGT CTCAGCTGCC
    CGGCTCCTTA GCCGTCAAAA CCTCCAGAGT CCTAAATCCA GTCGGTCAAC TATCTTCGTG
    CGTCTCCACA CCAACGGGAA CCAGGAGCTG CAGTACCAGC CTGGGGACCA CCTGGGTGTC
    TTCCCTGGCA ACCACGAGGA CCTCGTGAAT GCCCTGATCG AGCGGCTGGA GGACGCGCCC
    CCTGTCAACC AGATGGTGAA AGTGGAACTG CTGGAGGAGC GGAACACGGC TTTAGGTGTC
    ATCAGTAACT GGACAGACGA GCTCCGCCTC CCGCCCTGCA CCATCTTCCA GGCCTTCAAG
    TACTACCTGG ACATCACCAC GCCACCAACG CCCCTGCAGC TGCAGCAGTT TGCCTCCCTA
    GCTACCAGCG AGAAGGAGAA GCAGCGTCTG CTGGTCCTCA GCAAGGGTTT GCAGGAGTAC
    GAGGAATGGA AATGGGGCAA GAACCCCACC ATCGTGGAGG TGCTGGAGGA ATTCCCATCT
    ATCCAGATGC CGGCCACCCT GCTCCTGACC CAGCTGTCCC TGCTGCAGCC CCGCTACTAT
    TCCATCAGCT CCTCCCCAGA CATGTACCCT GATGAAGTGC ACCTCACTGT GGCCATCGTT
    TCCTACCGCA CTCGAGATGG AGAAGGACCA ATTCACCACG GCGTATGCTC CTCCTGGCTC
    AACCGGATAC AGGCTGACGA ACTGGTCCCC TGTTTCGTGA GAGGAGCACC CAGCTTCCAC
    CTGCCCCGGA ACCCCCAAGT CCCCTGCATC CTCGTTGGAC CAGGCACCGG CATTGCCCCT
    TTCCGAAGCT TCTGGCAACA GCGGCAATTT GATATCCAAC ACAAAGGAAT GAACCCCTGC
    CCCATGGTCC TGGTCTTCGG GTGCCGGCAA TCCAAGATAG ATCATATCTA CAGGGAAGAG
    ACCCTGCAGG CCAAGAACAA GGGGGTCTTC AGAGAGCTGT ACACGGCTTA CTCCCGGGAG
    CCAGACAAAC CAAAGAAGTA TGTGCAGGAC ATCCTGCAGG AGCAGCTGGC GGAGTCTGTG
    TACCGAGCCC TGAAGGAGCA AGGGGGCCAC ATATACGTCT GTGGGGACGT CACCATGGCT
    GCTGATGTCC TCAAAGCCAT CCAGCGCATC ATGACCCAGC AGGGGAAGCT CTCGGCAGAG
    GACGCCGGCG TATTCATCAG CCGGATGAGG GATGACAACC GATACCATGA GGATATTTTT
    GGAGTCACCC TGCGAACGTA CGAAGTGACC AACCGCCTTA GATCTGAGTC CATTGCCTTC
    ATTGAAGAGA GCAAAAAAGA CACCGATGAG GTTTTCAGCT CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009424621.2
    CDS712..5016
    Translation

    Target ORF information:

    RefSeq Version XM_009426346.2
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes nitric oxide synthase 1 (NOS1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009426346.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAGGATC ACATGTTCGG TGTTCAGCAA ATCCAGCCCA ATGTCATTTC TGTTCGTCTC 
    TTCAAGCGCA AAGTTGGGGG CCTGGGATTC CTGGTGAAGG AGCGGGTCAG TAAGCCGCCC
    GTGATTATCT CTGACCTGAT TCGTGGGGGC GCCGCAGAGC AGAGTGGCCT CATCCAGGCC
    GGAGACATCA TTCTTGCGGT CAACGGCCGG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC
    CTGGAGGTAC TCAGAGGCAT TGCCTCCGAG ACCCACGTGG TCCTCATTCT GAGGGGTCCT
    GAAGGTTTCA CCACGCACCT GGAGACCACC TTTACAGGTG ATGGGACCCC CAAGACCATC
    CGGGTGACAC AGCCCCTGGG TCCCCCCACC AAAGCCGTGG ATCTGTCCCA CCAGCCATCG
    GCCGGCAAAG AACAGCCGCT GGCAGTGGAT GGGGCCTCGG GTCCCGGGAA TGGGCCTCAG
    CATGCCTACG ATGACGGGCA GGAGGCTGGC TCACTCCCCC ATGCCAACGG CCTGGCCCCC
    AGGCCCCCAG GCCAGGACCC TGCGAAGAAA GCAACCAGAG TCAGCCTCCA AGGCAGAGGG
    GAGAACAATG AACTGCTCAA GGAGATAGAG CCTGTGCTGA GCCTTCTCAC CAGTGGGAGC
    AGAGGGGTCA AGGGAGGGGC ACCTGCCAAG GCAGAGATGA AAGATATGGG AATCCAGGTG
    GACAGAGATT TGGACGGCAA GTCACACAAA CCTCTGCCCC TCGCCGTGGA GAATGACCGA
    GTCTTCAATG ACCTATGGGG GAAGGGCAAT GTGCCTGTCG TCCTCAACAA CCCATATTCA
    GAGAAGGAGC AGCCCCCTGC CTCAGGAAAA CAGTCCCCCA CAAAGAATGG CAGCCCCTCC
    AAGTGTCCAC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG AGGTGGTTCT CACTGACACC
    CTCCACCTTA AGAGCACATT GGAAACGGGA TGCACTGAGT ACATCTGCAT GGGCTCCATC
    ATGCATCCTT CTCAGCATGC AAGGAGGCCT GAAGATGTCC GCACAAAAGA ACAGCTCTTC
    CCTCTCGCCA AAGAGTTTAT TGATCAATAC TATTCATCAA TTAAAAGATT TGGCTCCAAA
    GCCCACATGG AAAGGCTGGA AGAGGTGAAC AAAGAGATCG AAACCACTAG CACCTACCAG
    CTCAAGGACA CAGAGCTCAT CTATGGGGCC AAGCACGCCT GGCGGAATGC CTCGCGCTGT
    GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTATTCGATG CCCGTGACTG CACCACGGCC
    CACGGGATGT TCAACTACAT CTGTAACCAC GTCAAGTATG CCACCAACAA AGGGAACCTC
    AGGTCCGCCA TCACCATATT CCCCCAGAGG ACAGACGGCA AGCACGACTT CCGAGTCTGG
    AACTCCCAGC TCATCCGCTA CGCTGGCTAC AAGCAGCCCG ACGGCTCCAC CCTGGGGGAC
    CCAGCCAATG TGCAGTTCAC AGAGATATGC ATACAGCAGG GCTGGAAACC GCCTAGAGGC
    CGCTTCGATG TCCTGCCGCT CCTGCTTCAG GCCAACGGCA ATGACCCTGA GCTCTTCCAG
    ATTCCTCCAG AGCTGGTGTT GGAAGTTCCC ATCAGGCACC CCAAGTTTGA GTGGTTCAAG
    GACCTGGGGC TGAAGTGGTA CGGCCTCCCC GCCGTGTCCA ACATGCTCCT AGAGATTGGC
    GGCCTGGAGT TCAGCGCCTG TCCCTTCAGT GGCTGGTACA TGGGCACAGA GATTGGTGTC
    CGCGACTACT GTGACAACTC CCGCTACAAT ATCCTGGAGG AAGTGGCCAA GAAGATGAAC
    TTAGACATGA GGAAGATGTC CTCCCTGTGG AAGGACCAGG CGCTGGTGGA GATCAATATC
    GCGGTTCTCT ACAGCTTCCA GAGTGACAAA GTGACCATTG TTGACCATCA CTCCGCCACC
    GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGGGGCTG CCCTGCCGAC
    TGGGTGTGGA TCGTGCCCCC CATGTCCGGA AGCATCACCC CTGTGTTCCA CCAGGAGATG
    CTCAACTACC GGCTCACCCC CTCCTTCGAA TACCAGCCTG ATCCCTGGAA CACGCATGTC
    TGGAAAGGCA CCAACGGGAC CCCCACAAAG CGGCGAGCCA TCGGCTTCAA GAAGCTAGCA
    GAAGCTGTCA AGTTCTCGGC CAAGCTGATG GGGCAGGCTA TGGCCAAGAG GGTCAAAGCG
    ACCATCCTCT ATGCCACAGA GACAGGCAAA TCGCAAGCTT ATGCCAAGAC CTTGTGTGAG
    ATCTTCAAAC ACGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATCGTGCAC
    CTGGAACATG AAACTCTGGT CCTCGTGGTC ACCAGCACCT TTGGCAATGG AGATCCCCCT
    GAGAATGGGG AGAAATTCGG CTGTGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAG
    GAAGAAAGGA AGAGCTACAA GGTCCGATTC AACAGCGTCT CCTCCTACTC TGACTCCCAA
    AAATCATCAG GCGATGGGCC CGACCTCAGA GACAACTTTG AGAGTGCTGG ACCCCTGGCC
    AATGTGAGGT TCTCAGTTTT TGGCCTCGGC TCACGAGCAT ACCCTCACTT TTGTGCCTTC
    GGACACGCTG TGGACACCCT CCTGGAAGAA CTGGGAGGGG AGAGGATCCT GAAGATGAGG
    GAAGGGGATG AGCTCTGTGG GCAGGAAGAG GCTTTCAGGA CCTGGGCCAA GAAGGTCTTC
    AAGGCAGCCT GTGATGTCTT CTGTGTGGGA GATGATGTCA ACATTGAAAA GGCCAACAAT
    TCCCTCATCA GCAATGATCG CAGCTGGAAG AGAAACAAGT TCCGCCTCAC CTTTGTGGCC
    GAAGCTCCAG AACTCACACA AGGTCTATCC AATGTCCACA AAAAGCGAGT CTCAGCTGCC
    CGGCTCCTTA GCCGTCAAAA CCTCCAGAGT CCTAAATCCA GTCGGTCAAC TATCTTCGTG
    CGTCTCCACA CCAACGGGAA CCAGGAGCTG CAGTACCAGC CTGGGGACCA CCTGGGTGTC
    TTCCCTGGCA ACCACGAGGA CCTCGTGAAT GCCCTGATCG AGCGGCTGGA GGACGCGCCC
    CCTGTCAACC AGATGGTGAA AGTGGAACTG CTGGAGGAGC GGAACACGGC TTTAGGTGTC
    ATCAGTAACT GGACAGACGA GCTCCGCCTC CCGCCCTGCA CCATCTTCCA GGCCTTCAAG
    TACTACCTGG ACATCACCAC GCCACCAACG CCCCTGCAGC TGCAGCAGTT TGCCTCCCTA
    GCTACCAGCG AGAAGGAGAA GCAGCGTCTG CTGGTCCTCA GCAAGGGTTT GCAGGAGTAC
    GAGGAATGGA AATGGGGCAA GAACCCCACC ATCGTGGAGG TGCTGGAGGA ATTCCCATCT
    ATCCAGATGC CGGCCACCCT GCTCCTGACC CAGCTGTCCC TGCTGCAGCC CCGCTACTAT
    TCCATCAGCT CCTCCCCAGA CATGTACCCT GATGAAGTGC ACCTCACTGT GGCCATCGTT
    TCCTACCGCA CTCGAGATGG AGAAGGACCA ATTCACCACG GCGTATGCTC CTCCTGGCTC
    AACCGGATAC AGGCTGACGA ACTGGTCCCC TGTTTCGTGA GAGGAGCACC CAGCTTCCAC
    CTGCCCCGGA ACCCCCAAGT CCCCTGCATC CTCGTTGGAC CAGGCACCGG CATTGCCCCT
    TTCCGAAGCT TCTGGCAACA GCGGCAATTT GATATCCAAC ACAAAGGAAT GAACCCCTGC
    CCCATGGTCC TGGTCTTCGG GTGCCGGCAA TCCAAGATAG ATCATATCTA CAGGGAAGAG
    ACCCTGCAGG CCAAGAACAA GGGGGTCTTC AGAGAGCTGT ACACGGCTTA CTCCCGGGAG
    CCAGACAAAC CAAAGAAGTA TGTGCAGGAC ATCCTGCAGG AGCAGCTGGC GGAGTCTGTG
    TACCGAGCCC TGAAGGAGCA AGGGGGCCAC ATATACGTCT GTGGGGACGT CACCATGGCT
    GCTGATGTCC TCAAAGCCAT CCAGCGCATC ATGACCCAGC AGGGGAAGCT CTCGGCAGAG
    GACGCCGGCG TATTCATCAG CCGGATGAGG GATGACAACC GATACCATGA GGATATTTTT
    GGAGTCACCC TGCGAACGTA CGAAGTGACC AACCGCCTTA GATCTGAGTC CATTGCCTTC
    ATTGAAGAGA GCAAAAAAGA CACCGATGAG GTTTTCAGCT CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.