NOS1 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
NM_000620.4	NP_000611.1	nitric oxide synthase, brain isoform 1
NM_001204213.1	NP_001191142.1	nitric oxide synthase, brain isoform 3
XM_017019345.1	XP_016874834.1	nitric oxide synthase, brain isoform X1
XM_011538398.2	XP_011536700.1	nitric oxide synthase, brain isoform X1
NM_001204214.1	NP_001191143.1	nitric oxide synthase, brain isoform 3
NM_000620.5	NP_000611.1	nitric oxide synthase, brain isoform 1
XM_017019346.1	XP_016874835.1	nitric oxide synthase, brain isoform X1
XM_017019347.1	XP_016874836.1	nitric oxide synthase, brain isoform X2
NM_001204218.1	NP_001191147.1	nitric oxide synthase, brain isoform 2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu21527	NM_001204214.1 Latest version!	Homo sapiens nitric oxide synthase 1 (NOS1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu21527	NM_001204213.1 Latest version!	Homo sapiens nitric oxide synthase 1 (NOS1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu21020	NM_000620.5 Latest version!	Homo sapiens nitric oxide synthase 1 (NOS1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu21540	NM_001204218.1 Latest version!	Homo sapiens nitric oxide synthase 1 (NOS1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu21020	XM_017019347.1 Latest version!	Homo sapiens nitric oxide synthase 1 (NOS1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu21540	XM_011538398.2 Latest version!	Homo sapiens nitric oxide synthase 1 (NOS1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu21540	XM_017019346.1 Latest version!	Homo sapiens nitric oxide synthase 1 (NOS1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu21540	XM_017019345.1 Latest version!	Homo sapiens nitric oxide synthase 1 (NOS1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
View Old Accession Versions >>
OHu21020	NM_000620.4	Homo sapiens nitric oxide synthase 1 (NOS1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
Hide Old Accession Versions >>

NOS1 ( NM_001204213.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001191142.1 Homo sapiens nitric oxide synthase 1 (NOS1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu21527
Clone ID Related Accession (Same CDS sequence)	NM_001204213.1 , NM_001204214.1
Accession Version	NM_001204213.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3297bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	nitric oxide synthase, brain isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC068799.14, AK294435.1, U17327.1, AK307481.1, BC033208.1, AC026364.36 and BE207961.1. On Aug 31, 2013 this sequence version replaced XM_005253886.1. Transcript Variant: This variant (3, also known as TnNOS) contains 2 unique alternate exons (Tex 1 and Tex 2) at the 5' end compared to variant 1, resulting in translation initiation from a downstream AUG, and an isoform (isoform 3, also known as nNOSgamma) with a shorter N-terminus compared to isoform 1. This variant is specifically expressed in the testis, and the encoded isoform has catalytic activity (PMID:9111048). Variants 3 and 4 encode the same isoform. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03465413 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCTCCA TCATGCATCC TTCTCAGCAT GCAAGGAGGC CTGAAGACGT CCGCACAAAA 
GGACAGCTCT TCCCTCTCGC CAAAGAGTTT ATTGATCAAT ACTATTCATC AATTAAAAGA 
TTTGGCTCCA AAGCCCACAT GGAAAGGCTG GAAGAGGTGA ACAAAGAGAT CGACACCACT 
AGCACTTACC AGCTCAAGGA CACAGAGCTC ATCTATGGGG CCAAGCACGC CTGGCGGAAT 
GCCTCGCGCT GTGTGGGCAG GATCCAGTGG TCCAAGCTGC AGGTATTCGA TGCCCGTGAC 
TGCACCACGG CCCACGGGAT GTTCAACTAC ATCTGTAACC ATGTCAAGTA TGCCACCAAC 
AAAGGGAACC TCAGGTCTGC CATCACCATA TTCCCCCAGA GGACAGACGG CAAGCACGAC 
TTCCGAGTCT GGAACTCCCA GCTCATCCGC TACGCTGGCT ACAAGCAGCC TGACGGCTCC 
ACCCTGGGGG ACCCAGCCAA TGTGCAGTTC ACAGAGATAT GCATACAGCA GGGCTGGAAA 
CCGCCTAGAG GCCGCTTCGA TGTCCTGCCG CTCCTGCTTC AGGCCAACGG CAATGACCCT 
GAGCTCTTCC AGATTCCTCC AGAGCTGGTG TTGGAAGTTC CCATCAGGCA CCCCAAGTTT 
GAGTGGTTCA AGGACCTGGG GCTGAAGTGG TACGGCCTCC CCGCCGTGTC CAACATGCTC 
CTAGAGATTG GCGGCCTGGA GTTCAGCGCC TGTCCCTTCA GTGGCTGGTA CATGGGCACA 
GAGATTGGTG TCCGCGACTA CTGTGACAAC TCCCGCTACA ATATCCTGGA GGAAGTGGCC 
AAGAAGATGA ACTTAGACAT GAGGAAGACG TCCTCCCTGT GGAAGGACCA GGCGCTGGTG 
GAGATCAATA TCGCGGTTCT CTATAGCTTC CAGAGTGACA AAGTGACCAT TGTTGACCAT 
CACTCCGCCA CCGAGTCCTT CATTAAGCAC ATGGAGAATG AGTACCGCTG CCGGGGGGGC 
TGCCCTGCCG ACTGGGTGTG GATCGTGCCC CCCATGTCCG GAAGCATCAC CCCTGTGTTC 
CACCAGGAGA TGCTCAACTA CCGGCTCACC CCCTCCTTCG AATACCAGCC TGATCCCTGG 
AACACGCATG TCTGGAAAGG CACCAACGGG ACCCCCACAA AGCGGCGAGC CATTGGCTTC 
AAGAAGCTAG CAGAAGCTGT CAAGTTCTCG GCCAAGCTGA TGGGGCAGGC TATGGCCAAG 
AGGGTGAAAG CGACCATCCT CTATGCCACA GAGACAGGCA AATCGCAAGC TTATGCCAAG 
ACCTTGTGTG AGATCTTCAA ACACGCCTTT GATGCCAAGG TGATGTCCAT GGAAGAATAT 
GACATTGTGC ACCTGGAACA TGAAACTCTG GTCCTTGTGG TCACCAGCAC CTTTGGCAAT 
GGAGATCCCC CTGAGAATGG GGAGAAATTC GGCTGTGCTT TGATGGAAAT GAGGCACCCC 
AACTCTGTGC AGGAAGAAAG GAAGAGCTAC AAGGTCCGAT TCAACAGCGT CTCCTCCTAC 
TCTGACTCCC AAAAATCATC AGGCGATGGG CCCGACCTCA GAGACAACTT TGAGAGTGCT 
GGACCCCTGG CCAATGTGAG GTTCTCAGTT TTTGGCCTCG GCTCACGAGC ATACCCTCAC 
TTTTGCGCCT TCGGACACGC TGTGGACACC CTCCTGGAAG AACTGGGAGG GGAGAGGATC 
CTGAAGATGA GGGAAGGGGA TGAGCTCTGT GGGCAGGAAG AGGCTTTCAG GACCTGGGCC 
AAGAAGGTCT TCAAGGCAGC CTGTGATGTC TTCTGTGTGG GAGATGATGT CAACATTGAA 
AAGGCCAACA ATTCCCTCAT CAGCAATGAT CGCAGCTGGA AGAGAAACAA GTTCCGCCTC 
ACCTTTGTGG CCGAAGCTCC AGAACTCACA CAAGGTCTAT CCAATGTCCA CAAAAAGCGA 
GTCTCAGCTG CCCGGCTCCT TAGCCGTCAA AACCTCCAGA GCCCTAAATC CAGTCGGTCA 
ACTATCTTCG TGCGTCTCCA CACCAACGGG AGCCAGGAGC TGCAGTACCA GCCTGGGGAC 
CACCTGGGTG TCTTCCCTGG CAACCACGAG GACCTCGTGA ATGCCCTGAT CGAGCGGCTG 
GAGGACGCGC CGCCTGTCAA CCAGATGGTG AAAGTGGAAC TGCTGGAGGA GCGGAACACG 
GCTTTAGGTG TCATCAGTAA CTGGACAGAC GAGCTCCGCC TCCCGCCCTG CACCATCTTC 
CAGGCCTTCA AGTACTACCT GGACATCACC ACGCCACCAA CGCCTCTGCA GCTGCAGCAG 
TTTGCCTCCC TAGCTACCAG CGAGAAGGAG AAGCAGCGTC TGCTGGTCCT CAGCAAGGGT 
TTGCAGGAGT ACGAGGAATG GAAATGGGGC AAGAACCCCA CCATCGTGGA GGTGCTGGAG 
GAGTTCCCAT CTATCCAGAT GCCGGCCACC CTGCTCCTGA CCCAGCTGTC CCTGCTGCAG 
CCCCGCTACT ATTCCATCAG CTCCTCCCCA GACATGTACC CTGATGAAGT GCACCTCACT 
GTGGCCATCG TTTCCTACCG CACTCGAGAT GGAGAAGGAC CAATTCACCA CGGCGTATGC 
TCCTCCTGGC TCAACCGGAT ACAGGCTGAC GAACTGGTCC CCTGTTTCGT GAGAGGAGCA 
CCCAGCTTCC ACCTGCCCCG GAACCCCCAA GTCCCCTGCA TCCTCGTTGG ACCAGGCACC 
GGCATTGCCC CTTTCCGAAG CTTCTGGCAA CAGCGGCAAT TTGATATCCA ACACAAAGGA 
ATGAACCCCT GCCCCATGGT CCTGGTCTTC GGGTGCCGGC AATCCAAGAT AGATCATATC 
TACAGGGAAG AGACCCTGCA GGCCAAGAAC AAGGGGGTCT TCAGAGAGCT GTACACGGCT 
TACTCCCGGG AGCCAGACAA ACCAAAGAAG TACGTGCAGG ACATCCTGCA GGAGCAGCTG 
GCGGAGTCTG TGTACCGAGC CCTGAAGGAG CAAGGGGGCC ACATATACGT CTGTGGGGAC 
GTCACCATGG CTGCTGATGT CCTCAAAGCC ATCCAGCGCA TCATGACCCA GCAGGGGAAG 
CTCTCGGCAG AGGACGCCGG CGTATTCATC AGCCGGATGA GGGATGACAA CCGATACCAT 
GAGGATATTT TTGGAGTCAC CCTGCGAACG TACGAAGTGA CCAACCGCCT TAGATCTGAG 
TCCATTGCCT TCATTGAAGA GAGCAAAAAA GACACCGATG AGGTTTTCAG CTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001191142.1
CDS	312..3608
Translation	MGSIMHPSQHARRPEDVRTKGQLFPLAKEFIDQYYSSIKRFGSKAHMERLEEVNKEIDTTSTYQLKDTELIYGAKHAWRNASRCVGRIQWSKLQVFDARDCTTAHGMFNYICNHVKYATNKGNLRSAITIFPQRTDGKHDFRVWNSQLIRYAGYKQPDGSTLGDPANVQFTEICIQQGWKPPRGRFDVLPLLLQANGNDPELFQIPPELVLEVPIRHPKFEWFKDLGLKWYGLPAVSNMLLEIGGLEFSACPFSGWYMGTEIGVRDYCDNSRYNILEEVAKKMNLDMRKTSSLWKDQALVEINIAVLYSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMLNYRLTPSFEYQPDPWNTHVWKGTNGTPTKRRAIGFKKLAEAVKFSAKLMGQAMAKRVKATILYATETGKSQAYAKTLCEIFKHAFDAKVMSMEEYDIVHLEHETLVLVVTSTFGNGDPPENGEKFGCALMEMRHPNSVQEERKSYKVRFNSVSSYSDSQKSSGDGPDLRDNFESAGPLANVRFSVFGLGSRAYPHFCAFGHAVDTLLEELGGERILKMREGDELCGQEEAFRTWAKKVFKAACDVFCVGDDVNIEKANNSLISNDRSWKRNKFRLTFVAEAPELTQGLSNVHKKRVSAARLLSRQNLQSPKSSRSTIFVRLHTNGSQELQYQPGDHLGVFPGNHEDLVNALIERLEDAPPVNQMVKVELLEERNTALGVISNWTDELRLPPCTIFQAFKYYLDITTPPTPLQLQQFASLATSEKEKQRLLVLSKGLQEYEEWKWGKNPTIVEVLEEFPSIQMPATLLLTQLSLLQPRYYSISSSPDMYPDEVHLTVAIVSYRTRDGEGPIHHGVCSSWLNRIQADELVPCFVRGAPSFHLPRNPQVPCILVGPGTGIAPFRSFWQQRQFDIQHKGMNPCPMVLVFGCRQSKIDHIYREETLQAKNKGVFRELYTAYSREPDKPKKYVQDILQEQLAESVYRALKEQGGHIYVCGDVTMAADVLKAIQRIMTQQGKLSAEDAGVFISRMRDDNRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDTDEVFSS

Target ORF information:

RefSeq Version	NM_001204213.1
Organism	Homo sapiens(human)
Definition	Homo sapiens nitric oxide synthase 1 (NOS1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001204213.1

ORF Insert Sequence:

ATGGGCTCCA TCATGCATCC TTCTCAGCAT GCAAGGAGGC CTGAAGACGT CCGCACAAAA 
GGACAGCTCT TCCCTCTCGC CAAAGAGTTT ATTGATCAAT ACTATTCATC AATTAAAAGA 
TTTGGCTCCA AAGCCCACAT GGAAAGGCTG GAAGAGGTGA ACAAAGAGAT CGACACCACT 
AGCACTTACC AGCTCAAGGA CACAGAGCTC ATCTATGGGG CCAAGCACGC CTGGCGGAAT 
GCCTCGCGCT GTGTGGGCAG GATCCAGTGG TCCAAGCTGC AGGTATTCGA TGCCCGTGAC 
TGCACCACGG CCCACGGGAT GTTCAACTAC ATCTGTAACC ATGTCAAGTA TGCCACCAAC 
AAAGGGAACC TCAGGTCTGC CATCACCATA TTCCCCCAGA GGACAGACGG CAAGCACGAC 
TTCCGAGTCT GGAACTCCCA GCTCATCCGC TACGCTGGCT ACAAGCAGCC TGACGGCTCC 
ACCCTGGGGG ACCCAGCCAA TGTGCAGTTC ACAGAGATAT GCATACAGCA GGGCTGGAAA 
CCGCCTAGAG GCCGCTTCGA TGTCCTGCCG CTCCTGCTTC AGGCCAACGG CAATGACCCT 
GAGCTCTTCC AGATTCCTCC AGAGCTGGTG TTGGAAGTTC CCATCAGGCA CCCCAAGTTT 
GAGTGGTTCA AGGACCTGGG GCTGAAGTGG TACGGCCTCC CCGCCGTGTC CAACATGCTC 
CTAGAGATTG GCGGCCTGGA GTTCAGCGCC TGTCCCTTCA GTGGCTGGTA CATGGGCACA 
GAGATTGGTG TCCGCGACTA CTGTGACAAC TCCCGCTACA ATATCCTGGA GGAAGTGGCC 
AAGAAGATGA ACTTAGACAT GAGGAAGACG TCCTCCCTGT GGAAGGACCA GGCGCTGGTG 
GAGATCAATA TCGCGGTTCT CTATAGCTTC CAGAGTGACA AAGTGACCAT TGTTGACCAT 
CACTCCGCCA CCGAGTCCTT CATTAAGCAC ATGGAGAATG AGTACCGCTG CCGGGGGGGC 
TGCCCTGCCG ACTGGGTGTG GATCGTGCCC CCCATGTCCG GAAGCATCAC CCCTGTGTTC 
CACCAGGAGA TGCTCAACTA CCGGCTCACC CCCTCCTTCG AATACCAGCC TGATCCCTGG 
AACACGCATG TCTGGAAAGG CACCAACGGG ACCCCCACAA AGCGGCGAGC CATTGGCTTC 
AAGAAGCTAG CAGAAGCTGT CAAGTTCTCG GCCAAGCTGA TGGGGCAGGC TATGGCCAAG 
AGGGTGAAAG CGACCATCCT CTATGCCACA GAGACAGGCA AATCGCAAGC TTATGCCAAG 
ACCTTGTGTG AGATCTTCAA ACACGCCTTT GATGCCAAGG TGATGTCCAT GGAAGAATAT 
GACATTGTGC ACCTGGAACA TGAAACTCTG GTCCTTGTGG TCACCAGCAC CTTTGGCAAT 
GGAGATCCCC CTGAGAATGG GGAGAAATTC GGCTGTGCTT TGATGGAAAT GAGGCACCCC 
AACTCTGTGC AGGAAGAAAG GAAGAGCTAC AAGGTCCGAT TCAACAGCGT CTCCTCCTAC 
TCTGACTCCC AAAAATCATC AGGCGATGGG CCCGACCTCA GAGACAACTT TGAGAGTGCT 
GGACCCCTGG CCAATGTGAG GTTCTCAGTT TTTGGCCTCG GCTCACGAGC ATACCCTCAC 
TTTTGCGCCT TCGGACACGC TGTGGACACC CTCCTGGAAG AACTGGGAGG GGAGAGGATC 
CTGAAGATGA GGGAAGGGGA TGAGCTCTGT GGGCAGGAAG AGGCTTTCAG GACCTGGGCC 
AAGAAGGTCT TCAAGGCAGC CTGTGATGTC TTCTGTGTGG GAGATGATGT CAACATTGAA 
AAGGCCAACA ATTCCCTCAT CAGCAATGAT CGCAGCTGGA AGAGAAACAA GTTCCGCCTC 
ACCTTTGTGG CCGAAGCTCC AGAACTCACA CAAGGTCTAT CCAATGTCCA CAAAAAGCGA 
GTCTCAGCTG CCCGGCTCCT TAGCCGTCAA AACCTCCAGA GCCCTAAATC CAGTCGGTCA 
ACTATCTTCG TGCGTCTCCA CACCAACGGG AGCCAGGAGC TGCAGTACCA GCCTGGGGAC 
CACCTGGGTG TCTTCCCTGG CAACCACGAG GACCTCGTGA ATGCCCTGAT CGAGCGGCTG 
GAGGACGCGC CGCCTGTCAA CCAGATGGTG AAAGTGGAAC TGCTGGAGGA GCGGAACACG 
GCTTTAGGTG TCATCAGTAA CTGGACAGAC GAGCTCCGCC TCCCGCCCTG CACCATCTTC 
CAGGCCTTCA AGTACTACCT GGACATCACC ACGCCACCAA CGCCTCTGCA GCTGCAGCAG 
TTTGCCTCCC TAGCTACCAG CGAGAAGGAG AAGCAGCGTC TGCTGGTCCT CAGCAAGGGT 
TTGCAGGAGT ACGAGGAATG GAAATGGGGC AAGAACCCCA CCATCGTGGA GGTGCTGGAG 
GAGTTCCCAT CTATCCAGAT GCCGGCCACC CTGCTCCTGA CCCAGCTGTC CCTGCTGCAG 
CCCCGCTACT ATTCCATCAG CTCCTCCCCA GACATGTACC CTGATGAAGT GCACCTCACT 
GTGGCCATCG TTTCCTACCG CACTCGAGAT GGAGAAGGAC CAATTCACCA CGGCGTATGC 
TCCTCCTGGC TCAACCGGAT ACAGGCTGAC GAACTGGTCC CCTGTTTCGT GAGAGGAGCA 
CCCAGCTTCC ACCTGCCCCG GAACCCCCAA GTCCCCTGCA TCCTCGTTGG ACCAGGCACC 
GGCATTGCCC CTTTCCGAAG CTTCTGGCAA CAGCGGCAAT TTGATATCCA ACACAAAGGA 
ATGAACCCCT GCCCCATGGT CCTGGTCTTC GGGTGCCGGC AATCCAAGAT AGATCATATC 
TACAGGGAAG AGACCCTGCA GGCCAAGAAC AAGGGGGTCT TCAGAGAGCT GTACACGGCT 
TACTCCCGGG AGCCAGACAA ACCAAAGAAG TACGTGCAGG ACATCCTGCA GGAGCAGCTG 
GCGGAGTCTG TGTACCGAGC CCTGAAGGAG CAAGGGGGCC ACATATACGT CTGTGGGGAC 
GTCACCATGG CTGCTGATGT CCTCAAAGCC ATCCAGCGCA TCATGACCCA GCAGGGGAAG 
CTCTCGGCAG AGGACGCCGG CGTATTCATC AGCCGGATGA GGGATGACAA CCGATACCAT 
GAGGATATTT TTGGAGTCAC CCTGCGAACG TACGAAGTGA CCAACCGCCT TAGATCTGAG 
TCCATTGCCT TCATTGAAGA GAGCAAAAAA GACACCGATG AGGTTTTCAG CTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOS1 ( NM_001204214.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001191143.1 Homo sapiens nitric oxide synthase 1 (NOS1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu21527
Clone ID Related Accession (Same CDS sequence)	NM_001204213.1 , NM_001204214.1
Accession Version	NM_001204214.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3297bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	nitric oxide synthase, brain isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC068799.14, AK294435.1, U17327.1, AK307481.1, BC033208.1, AC026364.36 and BE207961.1. Transcript Variant: This variant (4, also known as TnNOSb) contains 2 unique alternate exons (Tex 1b and Tex 2) at the 5' end compared to variant 1, resulting in translation initiation from a downstream AUG, and an isoform (3, also known as nNOSgamma) with a shorter N-terminus compared to isoform 1. This variant is specifically expressed in the testis, and the encoded isoform has catalytic activity (PMID:9111048). Variants 3 and 4 encode the same isoform. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03465413 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCTCCA TCATGCATCC TTCTCAGCAT GCAAGGAGGC CTGAAGACGT CCGCACAAAA 
GGACAGCTCT TCCCTCTCGC CAAAGAGTTT ATTGATCAAT ACTATTCATC AATTAAAAGA 
TTTGGCTCCA AAGCCCACAT GGAAAGGCTG GAAGAGGTGA ACAAAGAGAT CGACACCACT 
AGCACTTACC AGCTCAAGGA CACAGAGCTC ATCTATGGGG CCAAGCACGC CTGGCGGAAT 
GCCTCGCGCT GTGTGGGCAG GATCCAGTGG TCCAAGCTGC AGGTATTCGA TGCCCGTGAC 
TGCACCACGG CCCACGGGAT GTTCAACTAC ATCTGTAACC ATGTCAAGTA TGCCACCAAC 
AAAGGGAACC TCAGGTCTGC CATCACCATA TTCCCCCAGA GGACAGACGG CAAGCACGAC 
TTCCGAGTCT GGAACTCCCA GCTCATCCGC TACGCTGGCT ACAAGCAGCC TGACGGCTCC 
ACCCTGGGGG ACCCAGCCAA TGTGCAGTTC ACAGAGATAT GCATACAGCA GGGCTGGAAA 
CCGCCTAGAG GCCGCTTCGA TGTCCTGCCG CTCCTGCTTC AGGCCAACGG CAATGACCCT 
GAGCTCTTCC AGATTCCTCC AGAGCTGGTG TTGGAAGTTC CCATCAGGCA CCCCAAGTTT 
GAGTGGTTCA AGGACCTGGG GCTGAAGTGG TACGGCCTCC CCGCCGTGTC CAACATGCTC 
CTAGAGATTG GCGGCCTGGA GTTCAGCGCC TGTCCCTTCA GTGGCTGGTA CATGGGCACA 
GAGATTGGTG TCCGCGACTA CTGTGACAAC TCCCGCTACA ATATCCTGGA GGAAGTGGCC 
AAGAAGATGA ACTTAGACAT GAGGAAGACG TCCTCCCTGT GGAAGGACCA GGCGCTGGTG 
GAGATCAATA TCGCGGTTCT CTATAGCTTC CAGAGTGACA AAGTGACCAT TGTTGACCAT 
CACTCCGCCA CCGAGTCCTT CATTAAGCAC ATGGAGAATG AGTACCGCTG CCGGGGGGGC 
TGCCCTGCCG ACTGGGTGTG GATCGTGCCC CCCATGTCCG GAAGCATCAC CCCTGTGTTC 
CACCAGGAGA TGCTCAACTA CCGGCTCACC CCCTCCTTCG AATACCAGCC TGATCCCTGG 
AACACGCATG TCTGGAAAGG CACCAACGGG ACCCCCACAA AGCGGCGAGC CATTGGCTTC 
AAGAAGCTAG CAGAAGCTGT CAAGTTCTCG GCCAAGCTGA TGGGGCAGGC TATGGCCAAG 
AGGGTGAAAG CGACCATCCT CTATGCCACA GAGACAGGCA AATCGCAAGC TTATGCCAAG 
ACCTTGTGTG AGATCTTCAA ACACGCCTTT GATGCCAAGG TGATGTCCAT GGAAGAATAT 
GACATTGTGC ACCTGGAACA TGAAACTCTG GTCCTTGTGG TCACCAGCAC CTTTGGCAAT 
GGAGATCCCC CTGAGAATGG GGAGAAATTC GGCTGTGCTT TGATGGAAAT GAGGCACCCC 
AACTCTGTGC AGGAAGAAAG GAAGAGCTAC AAGGTCCGAT TCAACAGCGT CTCCTCCTAC 
TCTGACTCCC AAAAATCATC AGGCGATGGG CCCGACCTCA GAGACAACTT TGAGAGTGCT 
GGACCCCTGG CCAATGTGAG GTTCTCAGTT TTTGGCCTCG GCTCACGAGC ATACCCTCAC 
TTTTGCGCCT TCGGACACGC TGTGGACACC CTCCTGGAAG AACTGGGAGG GGAGAGGATC 
CTGAAGATGA GGGAAGGGGA TGAGCTCTGT GGGCAGGAAG AGGCTTTCAG GACCTGGGCC 
AAGAAGGTCT TCAAGGCAGC CTGTGATGTC TTCTGTGTGG GAGATGATGT CAACATTGAA 
AAGGCCAACA ATTCCCTCAT CAGCAATGAT CGCAGCTGGA AGAGAAACAA GTTCCGCCTC 
ACCTTTGTGG CCGAAGCTCC AGAACTCACA CAAGGTCTAT CCAATGTCCA CAAAAAGCGA 
GTCTCAGCTG CCCGGCTCCT TAGCCGTCAA AACCTCCAGA GCCCTAAATC CAGTCGGTCA 
ACTATCTTCG TGCGTCTCCA CACCAACGGG AGCCAGGAGC TGCAGTACCA GCCTGGGGAC 
CACCTGGGTG TCTTCCCTGG CAACCACGAG GACCTCGTGA ATGCCCTGAT CGAGCGGCTG 
GAGGACGCGC CGCCTGTCAA CCAGATGGTG AAAGTGGAAC TGCTGGAGGA GCGGAACACG 
GCTTTAGGTG TCATCAGTAA CTGGACAGAC GAGCTCCGCC TCCCGCCCTG CACCATCTTC 
CAGGCCTTCA AGTACTACCT GGACATCACC ACGCCACCAA CGCCTCTGCA GCTGCAGCAG 
TTTGCCTCCC TAGCTACCAG CGAGAAGGAG AAGCAGCGTC TGCTGGTCCT CAGCAAGGGT 
TTGCAGGAGT ACGAGGAATG GAAATGGGGC AAGAACCCCA CCATCGTGGA GGTGCTGGAG 
GAGTTCCCAT CTATCCAGAT GCCGGCCACC CTGCTCCTGA CCCAGCTGTC CCTGCTGCAG 
CCCCGCTACT ATTCCATCAG CTCCTCCCCA GACATGTACC CTGATGAAGT GCACCTCACT 
GTGGCCATCG TTTCCTACCG CACTCGAGAT GGAGAAGGAC CAATTCACCA CGGCGTATGC 
TCCTCCTGGC TCAACCGGAT ACAGGCTGAC GAACTGGTCC CCTGTTTCGT GAGAGGAGCA 
CCCAGCTTCC ACCTGCCCCG GAACCCCCAA GTCCCCTGCA TCCTCGTTGG ACCAGGCACC 
GGCATTGCCC CTTTCCGAAG CTTCTGGCAA CAGCGGCAAT TTGATATCCA ACACAAAGGA 
ATGAACCCCT GCCCCATGGT CCTGGTCTTC GGGTGCCGGC AATCCAAGAT AGATCATATC 
TACAGGGAAG AGACCCTGCA GGCCAAGAAC AAGGGGGTCT TCAGAGAGCT GTACACGGCT 
TACTCCCGGG AGCCAGACAA ACCAAAGAAG TACGTGCAGG ACATCCTGCA GGAGCAGCTG 
GCGGAGTCTG TGTACCGAGC CCTGAAGGAG CAAGGGGGCC ACATATACGT CTGTGGGGAC 
GTCACCATGG CTGCTGATGT CCTCAAAGCC ATCCAGCGCA TCATGACCCA GCAGGGGAAG 
CTCTCGGCAG AGGACGCCGG CGTATTCATC AGCCGGATGA GGGATGACAA CCGATACCAT 
GAGGATATTT TTGGAGTCAC CCTGCGAACG TACGAAGTGA CCAACCGCCT TAGATCTGAG 
TCCATTGCCT TCATTGAAGA GAGCAAAAAA GACACCGATG AGGTTTTCAG CTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001191143.1
CDS	307..3603
Translation	MGSIMHPSQHARRPEDVRTKGQLFPLAKEFIDQYYSSIKRFGSKAHMERLEEVNKEIDTTSTYQLKDTELIYGAKHAWRNASRCVGRIQWSKLQVFDARDCTTAHGMFNYICNHVKYATNKGNLRSAITIFPQRTDGKHDFRVWNSQLIRYAGYKQPDGSTLGDPANVQFTEICIQQGWKPPRGRFDVLPLLLQANGNDPELFQIPPELVLEVPIRHPKFEWFKDLGLKWYGLPAVSNMLLEIGGLEFSACPFSGWYMGTEIGVRDYCDNSRYNILEEVAKKMNLDMRKTSSLWKDQALVEINIAVLYSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMLNYRLTPSFEYQPDPWNTHVWKGTNGTPTKRRAIGFKKLAEAVKFSAKLMGQAMAKRVKATILYATETGKSQAYAKTLCEIFKHAFDAKVMSMEEYDIVHLEHETLVLVVTSTFGNGDPPENGEKFGCALMEMRHPNSVQEERKSYKVRFNSVSSYSDSQKSSGDGPDLRDNFESAGPLANVRFSVFGLGSRAYPHFCAFGHAVDTLLEELGGERILKMREGDELCGQEEAFRTWAKKVFKAACDVFCVGDDVNIEKANNSLISNDRSWKRNKFRLTFVAEAPELTQGLSNVHKKRVSAARLLSRQNLQSPKSSRSTIFVRLHTNGSQELQYQPGDHLGVFPGNHEDLVNALIERLEDAPPVNQMVKVELLEERNTALGVISNWTDELRLPPCTIFQAFKYYLDITTPPTPLQLQQFASLATSEKEKQRLLVLSKGLQEYEEWKWGKNPTIVEVLEEFPSIQMPATLLLTQLSLLQPRYYSISSSPDMYPDEVHLTVAIVSYRTRDGEGPIHHGVCSSWLNRIQADELVPCFVRGAPSFHLPRNPQVPCILVGPGTGIAPFRSFWQQRQFDIQHKGMNPCPMVLVFGCRQSKIDHIYREETLQAKNKGVFRELYTAYSREPDKPKKYVQDILQEQLAESVYRALKEQGGHIYVCGDVTMAADVLKAIQRIMTQQGKLSAEDAGVFISRMRDDNRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDTDEVFSS

Target ORF information:

RefSeq Version	NM_001204214.1
Organism	Homo sapiens(human)
Definition	Homo sapiens nitric oxide synthase 1 (NOS1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001204214.1

ORF Insert Sequence:

ATGGGCTCCA TCATGCATCC TTCTCAGCAT GCAAGGAGGC CTGAAGACGT CCGCACAAAA 
GGACAGCTCT TCCCTCTCGC CAAAGAGTTT ATTGATCAAT ACTATTCATC AATTAAAAGA 
TTTGGCTCCA AAGCCCACAT GGAAAGGCTG GAAGAGGTGA ACAAAGAGAT CGACACCACT 
AGCACTTACC AGCTCAAGGA CACAGAGCTC ATCTATGGGG CCAAGCACGC CTGGCGGAAT 
GCCTCGCGCT GTGTGGGCAG GATCCAGTGG TCCAAGCTGC AGGTATTCGA TGCCCGTGAC 
TGCACCACGG CCCACGGGAT GTTCAACTAC ATCTGTAACC ATGTCAAGTA TGCCACCAAC 
AAAGGGAACC TCAGGTCTGC CATCACCATA TTCCCCCAGA GGACAGACGG CAAGCACGAC 
TTCCGAGTCT GGAACTCCCA GCTCATCCGC TACGCTGGCT ACAAGCAGCC TGACGGCTCC 
ACCCTGGGGG ACCCAGCCAA TGTGCAGTTC ACAGAGATAT GCATACAGCA GGGCTGGAAA 
CCGCCTAGAG GCCGCTTCGA TGTCCTGCCG CTCCTGCTTC AGGCCAACGG CAATGACCCT 
GAGCTCTTCC AGATTCCTCC AGAGCTGGTG TTGGAAGTTC CCATCAGGCA CCCCAAGTTT 
GAGTGGTTCA AGGACCTGGG GCTGAAGTGG TACGGCCTCC CCGCCGTGTC CAACATGCTC 
CTAGAGATTG GCGGCCTGGA GTTCAGCGCC TGTCCCTTCA GTGGCTGGTA CATGGGCACA 
GAGATTGGTG TCCGCGACTA CTGTGACAAC TCCCGCTACA ATATCCTGGA GGAAGTGGCC 
AAGAAGATGA ACTTAGACAT GAGGAAGACG TCCTCCCTGT GGAAGGACCA GGCGCTGGTG 
GAGATCAATA TCGCGGTTCT CTATAGCTTC CAGAGTGACA AAGTGACCAT TGTTGACCAT 
CACTCCGCCA CCGAGTCCTT CATTAAGCAC ATGGAGAATG AGTACCGCTG CCGGGGGGGC 
TGCCCTGCCG ACTGGGTGTG GATCGTGCCC CCCATGTCCG GAAGCATCAC CCCTGTGTTC 
CACCAGGAGA TGCTCAACTA CCGGCTCACC CCCTCCTTCG AATACCAGCC TGATCCCTGG 
AACACGCATG TCTGGAAAGG CACCAACGGG ACCCCCACAA AGCGGCGAGC CATTGGCTTC 
AAGAAGCTAG CAGAAGCTGT CAAGTTCTCG GCCAAGCTGA TGGGGCAGGC TATGGCCAAG 
AGGGTGAAAG CGACCATCCT CTATGCCACA GAGACAGGCA AATCGCAAGC TTATGCCAAG 
ACCTTGTGTG AGATCTTCAA ACACGCCTTT GATGCCAAGG TGATGTCCAT GGAAGAATAT 
GACATTGTGC ACCTGGAACA TGAAACTCTG GTCCTTGTGG TCACCAGCAC CTTTGGCAAT 
GGAGATCCCC CTGAGAATGG GGAGAAATTC GGCTGTGCTT TGATGGAAAT GAGGCACCCC 
AACTCTGTGC AGGAAGAAAG GAAGAGCTAC AAGGTCCGAT TCAACAGCGT CTCCTCCTAC 
TCTGACTCCC AAAAATCATC AGGCGATGGG CCCGACCTCA GAGACAACTT TGAGAGTGCT 
GGACCCCTGG CCAATGTGAG GTTCTCAGTT TTTGGCCTCG GCTCACGAGC ATACCCTCAC 
TTTTGCGCCT TCGGACACGC TGTGGACACC CTCCTGGAAG AACTGGGAGG GGAGAGGATC 
CTGAAGATGA GGGAAGGGGA TGAGCTCTGT GGGCAGGAAG AGGCTTTCAG GACCTGGGCC 
AAGAAGGTCT TCAAGGCAGC CTGTGATGTC TTCTGTGTGG GAGATGATGT CAACATTGAA 
AAGGCCAACA ATTCCCTCAT CAGCAATGAT CGCAGCTGGA AGAGAAACAA GTTCCGCCTC 
ACCTTTGTGG CCGAAGCTCC AGAACTCACA CAAGGTCTAT CCAATGTCCA CAAAAAGCGA 
GTCTCAGCTG CCCGGCTCCT TAGCCGTCAA AACCTCCAGA GCCCTAAATC CAGTCGGTCA 
ACTATCTTCG TGCGTCTCCA CACCAACGGG AGCCAGGAGC TGCAGTACCA GCCTGGGGAC 
CACCTGGGTG TCTTCCCTGG CAACCACGAG GACCTCGTGA ATGCCCTGAT CGAGCGGCTG 
GAGGACGCGC CGCCTGTCAA CCAGATGGTG AAAGTGGAAC TGCTGGAGGA GCGGAACACG 
GCTTTAGGTG TCATCAGTAA CTGGACAGAC GAGCTCCGCC TCCCGCCCTG CACCATCTTC 
CAGGCCTTCA AGTACTACCT GGACATCACC ACGCCACCAA CGCCTCTGCA GCTGCAGCAG 
TTTGCCTCCC TAGCTACCAG CGAGAAGGAG AAGCAGCGTC TGCTGGTCCT CAGCAAGGGT 
TTGCAGGAGT ACGAGGAATG GAAATGGGGC AAGAACCCCA CCATCGTGGA GGTGCTGGAG 
GAGTTCCCAT CTATCCAGAT GCCGGCCACC CTGCTCCTGA CCCAGCTGTC CCTGCTGCAG 
CCCCGCTACT ATTCCATCAG CTCCTCCCCA GACATGTACC CTGATGAAGT GCACCTCACT 
GTGGCCATCG TTTCCTACCG CACTCGAGAT GGAGAAGGAC CAATTCACCA CGGCGTATGC 
TCCTCCTGGC TCAACCGGAT ACAGGCTGAC GAACTGGTCC CCTGTTTCGT GAGAGGAGCA 
CCCAGCTTCC ACCTGCCCCG GAACCCCCAA GTCCCCTGCA TCCTCGTTGG ACCAGGCACC 
GGCATTGCCC CTTTCCGAAG CTTCTGGCAA CAGCGGCAAT TTGATATCCA ACACAAAGGA 
ATGAACCCCT GCCCCATGGT CCTGGTCTTC GGGTGCCGGC AATCCAAGAT AGATCATATC 
TACAGGGAAG AGACCCTGCA GGCCAAGAAC AAGGGGGTCT TCAGAGAGCT GTACACGGCT 
TACTCCCGGG AGCCAGACAA ACCAAAGAAG TACGTGCAGG ACATCCTGCA GGAGCAGCTG 
GCGGAGTCTG TGTACCGAGC CCTGAAGGAG CAAGGGGGCC ACATATACGT CTGTGGGGAC 
GTCACCATGG CTGCTGATGT CCTCAAAGCC ATCCAGCGCA TCATGACCCA GCAGGGGAAG 
CTCTCGGCAG AGGACGCCGG CGTATTCATC AGCCGGATGA GGGATGACAA CCGATACCAT 
GAGGATATTT TTGGAGTCAC CCTGCGAACG TACGAAGTGA CCAACCGCCT TAGATCTGAG 
TCCATTGCCT TCATTGAAGA GAGCAAAAAA GACACCGATG AGGTTTTCAG CTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOS1 ( NM_000620.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000611.1 Homo sapiens nitric oxide synthase 1 (NOS1), transcript variant 1, mRNA.

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CloneID	OHu21020
Clone ID Related Accession (Same CDS sequence)	NM_000620.4 , NM_000620.5 , XM_017019347.1
Accession Version	NM_000620.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4305bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-28
Organism	Homo sapiens(human)
Product	nitric oxide synthase, brain isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK307481.1, U17327.1, AK294435.1, BC033208.1, AC026364.36 and BE207961.1. This sequence is a reference standard in the RefSeqGene project. On Feb 18, 2011 this sequence version replaced NM_000620.3. Transcript Variant: This variant (1) represents the predominant transcript, and encodes isoform 1 (also known as nNOS). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU753241.1, U17327.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03465402, SAMN03465403 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGATC ACATGTTCGG TGTTCAGCAA ATCCAGCCCA ATGTCATTTC TGTTCGTCTC 
TTCAAGCGCA AAGTTGGGGG CCTGGGATTT CTGGTGAAGG AGCGGGTCAG TAAGCCGCCC 
GTGATCATCT CTGACCTGAT TCGTGGGGGC GCCGCAGAGC AGAGTGGCCT CATCCAGGCC 
GGAGACATCA TTCTTGCGGT CAACGGCCGG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC 
CTGGAGGTAC TCAGAGGCAT TGCCTCTGAG ACCCACGTGG TCCTCATTCT GAGGGGCCCT 
GAAGGTTTCA CCACGCACCT GGAGACCACC TTTACAGGTG ATGGGACCCC CAAGACCATC 
CGGGTGACAC AGCCCCTGGG TCCCCCCACC AAAGCCGTGG ATCTGTCCCA CCAGCCACCG 
GCCGGCAAAG AACAGCCCCT GGCAGTGGAT GGGGCCTCGG GTCCCGGGAA TGGGCCTCAG 
CATGCCTACG ATGATGGGCA GGAGGCTGGC TCACTCCCCC ATGCCAACGG CCTGGCCCCC 
AGGCCCCCAG GCCAGGACCC CGCGAAGAAA GCAACCAGAG TCAGCCTCCA AGGCAGAGGG 
GAGAACAATG AACTGCTCAA GGAGATAGAG CCTGTGCTGA GCCTTCTCAC CAGTGGGAGC 
AGAGGGGTCA AGGGAGGGGC ACCTGCCAAG GCAGAGATGA AAGATATGGG AATCCAGGTG 
GACAGAGATT TGGACGGCAA GTCACACAAA CCTCTGCCCC TCGGCGTGGA GAACGACCGA 
GTCTTCAATG ACCTATGGGG GAAGGGCAAT GTGCCTGTCG TCCTCAACAA CCCATATTCA 
GAGAAGGAGC AGCCCCCCAC CTCAGGAAAA CAGTCCCCCA CAAAGAATGG CAGCCCCTCC 
AAGTGTCCAC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG AGGTGGTTCT CACTGACACC 
CTCCACCTTA AGAGCACATT GGAAACGGGA TGCACTGAGT ACATCTGCAT GGGCTCCATC 
ATGCATCCTT CTCAGCATGC AAGGAGGCCT GAAGACGTCC GCACAAAAGG ACAGCTCTTC 
CCTCTCGCCA AAGAGTTTAT TGATCAATAC TATTCATCAA TTAAAAGATT TGGCTCCAAA 
GCCCACATGG AAAGGCTGGA AGAGGTGAAC AAAGAGATCG ACACCACTAG CACTTACCAG 
CTCAAGGACA CAGAGCTCAT CTATGGGGCC AAGCACGCCT GGCGGAATGC CTCGCGCTGT 
GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTATTCGATG CCCGTGACTG CACCACGGCC 
CACGGGATGT TCAACTACAT CTGTAACCAT GTCAAGTATG CCACCAACAA AGGGAACCTC 
AGGTCTGCCA TCACCATATT CCCCCAGAGG ACAGACGGCA AGCACGACTT CCGAGTCTGG 
AACTCCCAGC TCATCCGCTA CGCTGGCTAC AAGCAGCCTG ACGGCTCCAC CCTGGGGGAC 
CCAGCCAATG TGCAGTTCAC AGAGATATGC ATACAGCAGG GCTGGAAACC GCCTAGAGGC 
CGCTTCGATG TCCTGCCGCT CCTGCTTCAG GCCAACGGCA ATGACCCTGA GCTCTTCCAG 
ATTCCTCCAG AGCTGGTGTT GGAAGTTCCC ATCAGGCACC CCAAGTTTGA GTGGTTCAAG 
GACCTGGGGC TGAAGTGGTA CGGCCTCCCC GCCGTGTCCA ACATGCTCCT AGAGATTGGC 
GGCCTGGAGT TCAGCGCCTG TCCCTTCAGT GGCTGGTACA TGGGCACAGA GATTGGTGTC 
CGCGACTACT GTGACAACTC CCGCTACAAT ATCCTGGAGG AAGTGGCCAA GAAGATGAAC 
TTAGACATGA GGAAGACGTC CTCCCTGTGG AAGGACCAGG CGCTGGTGGA GATCAATATC 
GCGGTTCTCT ATAGCTTCCA GAGTGACAAA GTGACCATTG TTGACCATCA CTCCGCCACC 
GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGGGGCTG CCCTGCCGAC 
TGGGTGTGGA TCGTGCCCCC CATGTCCGGA AGCATCACCC CTGTGTTCCA CCAGGAGATG 
CTCAACTACC GGCTCACCCC CTCCTTCGAA TACCAGCCTG ATCCCTGGAA CACGCATGTC 
TGGAAAGGCA CCAACGGGAC CCCCACAAAG CGGCGAGCCA TTGGCTTCAA GAAGCTAGCA 
GAAGCTGTCA AGTTCTCGGC CAAGCTGATG GGGCAGGCTA TGGCCAAGAG GGTGAAAGCG 
ACCATCCTCT ATGCCACAGA GACAGGCAAA TCGCAAGCTT ATGCCAAGAC CTTGTGTGAG 
ATCTTCAAAC ACGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATTGTGCAC 
CTGGAACATG AAACTCTGGT CCTTGTGGTC ACCAGCACCT TTGGCAATGG AGATCCCCCT 
GAGAATGGGG AGAAATTCGG CTGTGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAG 
GAAGAAAGGA AGAGCTACAA GGTCCGATTC AACAGCGTCT CCTCCTACTC TGACTCCCAA 
AAATCATCAG GCGATGGGCC CGACCTCAGA GACAACTTTG AGAGTGCTGG ACCCCTGGCC 
AATGTGAGGT TCTCAGTTTT TGGCCTCGGC TCACGAGCAT ACCCTCACTT TTGCGCCTTC 
GGACACGCTG TGGACACCCT CCTGGAAGAA CTGGGAGGGG AGAGGATCCT GAAGATGAGG 
GAAGGGGATG AGCTCTGTGG GCAGGAAGAG GCTTTCAGGA CCTGGGCCAA GAAGGTCTTC 
AAGGCAGCCT GTGATGTCTT CTGTGTGGGA GATGATGTCA ACATTGAAAA GGCCAACAAT 
TCCCTCATCA GCAATGATCG CAGCTGGAAG AGAAACAAGT TCCGCCTCAC CTTTGTGGCC 
GAAGCTCCAG AACTCACACA AGGTCTATCC AATGTCCACA AAAAGCGAGT CTCAGCTGCC 
CGGCTCCTTA GCCGTCAAAA CCTCCAGAGC CCTAAATCCA GTCGGTCAAC TATCTTCGTG 
CGTCTCCACA CCAACGGGAG CCAGGAGCTG CAGTACCAGC CTGGGGACCA CCTGGGTGTC 
TTCCCTGGCA ACCACGAGGA CCTCGTGAAT GCCCTGATCG AGCGGCTGGA GGACGCGCCG 
CCTGTCAACC AGATGGTGAA AGTGGAACTG CTGGAGGAGC GGAACACGGC TTTAGGTGTC 
ATCAGTAACT GGACAGACGA GCTCCGCCTC CCGCCCTGCA CCATCTTCCA GGCCTTCAAG 
TACTACCTGG ACATCACCAC GCCACCAACG CCTCTGCAGC TGCAGCAGTT TGCCTCCCTA 
GCTACCAGCG AGAAGGAGAA GCAGCGTCTG CTGGTCCTCA GCAAGGGTTT GCAGGAGTAC 
GAGGAATGGA AATGGGGCAA GAACCCCACC ATCGTGGAGG TGCTGGAGGA GTTCCCATCT 
ATCCAGATGC CGGCCACCCT GCTCCTGACC CAGCTGTCCC TGCTGCAGCC CCGCTACTAT 
TCCATCAGCT CCTCCCCAGA CATGTACCCT GATGAAGTGC ACCTCACTGT GGCCATCGTT 
TCCTACCGCA CTCGAGATGG AGAAGGACCA ATTCACCACG GCGTATGCTC CTCCTGGCTC 
AACCGGATAC AGGCTGACGA ACTGGTCCCC TGTTTCGTGA GAGGAGCACC CAGCTTCCAC 
CTGCCCCGGA ACCCCCAAGT CCCCTGCATC CTCGTTGGAC CAGGCACCGG CATTGCCCCT 
TTCCGAAGCT TCTGGCAACA GCGGCAATTT GATATCCAAC ACAAAGGAAT GAACCCCTGC 
CCCATGGTCC TGGTCTTCGG GTGCCGGCAA TCCAAGATAG ATCATATCTA CAGGGAAGAG 
ACCCTGCAGG CCAAGAACAA GGGGGTCTTC AGAGAGCTGT ACACGGCTTA CTCCCGGGAG 
CCAGACAAAC CAAAGAAGTA CGTGCAGGAC ATCCTGCAGG AGCAGCTGGC GGAGTCTGTG 
TACCGAGCCC TGAAGGAGCA AGGGGGCCAC ATATACGTCT GTGGGGACGT CACCATGGCT 
GCTGATGTCC TCAAAGCCAT CCAGCGCATC ATGACCCAGC AGGGGAAGCT CTCGGCAGAG 
GACGCCGGCG TATTCATCAG CCGGATGAGG GATGACAACC GATACCATGA GGATATTTTT 
GGAGTCACCC TGCGAACGTA CGAAGTGACC AACCGCCTTA GATCTGAGTC CATTGCCTTC 
ATTGAAGAGA GCAAAAAAGA CACCGATGAG GTTTTCAGCT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000611.1
CDS	712..5016
Translation	MEDHMFGVQQIQPNVISVRLFKRKVGGLGFLVKERVSKPPVIISDLIRGGAAEQSGLIQAGDIILAVNGRPLVDLSYDSALEVLRGIASETHVVLILRGPEGFTTHLETTFTGDGTPKTIRVTQPLGPPTKAVDLSHQPPAGKEQPLAVDGASGPGNGPQHAYDDGQEAGSLPHANGLAPRPPGQDPAKKATRVSLQGRGENNELLKEIEPVLSLLTSGSRGVKGGAPAKAEMKDMGIQVDRDLDGKSHKPLPLGVENDRVFNDLWGKGNVPVVLNNPYSEKEQPPTSGKQSPTKNGSPSKCPRFLKVKNWETEVVLTDTLHLKSTLETGCTEYICMGSIMHPSQHARRPEDVRTKGQLFPLAKEFIDQYYSSIKRFGSKAHMERLEEVNKEIDTTSTYQLKDTELIYGAKHAWRNASRCVGRIQWSKLQVFDARDCTTAHGMFNYICNHVKYATNKGNLRSAITIFPQRTDGKHDFRVWNSQLIRYAGYKQPDGSTLGDPANVQFTEICIQQGWKPPRGRFDVLPLLLQANGNDPELFQIPPELVLEVPIRHPKFEWFKDLGLKWYGLPAVSNMLLEIGGLEFSACPFSGWYMGTEIGVRDYCDNSRYNILEEVAKKMNLDMRKTSSLWKDQALVEINIAVLYSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMLNYRLTPSFEYQPDPWNTHVWKGTNGTPTKRRAIGFKKLAEAVKFSAKLMGQAMAKRVKATILYATETGKSQAYAKTLCEIFKHAFDAKVMSMEEYDIVHLEHETLVLVVTSTFGNGDPPENGEKFGCALMEMRHPNSVQEERKSYKVRFNSVSSYSDSQKSSGDGPDLRDNFESAGPLANVRFSVFGLGSRAYPHFCAFGHAVDTLLEELGGERILKMREGDELCGQEEAFRTWAKKVFKAACDVFCVGDDVNIEKANNSLISNDRSWKRNKFRLTFVAEAPELTQGLSNVHKKRVSAARLLSRQNLQSPKSSRSTIFVRLHTNGSQELQYQPGDHLGVFPGNHEDLVNALIERLEDAPPVNQMVKVELLEERNTALGVISNWTDELRLPPCTIFQAFKYYLDITTPPTPLQLQQFASLATSEKEKQRLLVLSKGLQEYEEWKWGKNPTIVEVLEEFPSIQMPATLLLTQLSLLQPRYYSISSSPDMYPDEVHLTVAIVSYRTRDGEGPIHHGVCSSWLNRIQADELVPCFVRGAPSFHLPRNPQVPCILVGPGTGIAPFRSFWQQRQFDIQHKGMNPCPMVLVFGCRQSKIDHIYREETLQAKNKGVFRELYTAYSREPDKPKKYVQDILQEQLAESVYRALKEQGGHIYVCGDVTMAADVLKAIQRIMTQQGKLSAEDAGVFISRMRDDNRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDTDEVFSS

Target ORF information:

RefSeq Version	NM_000620.4
Organism	Homo sapiens(human)
Definition	Homo sapiens nitric oxide synthase 1 (NOS1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000620.4

ORF Insert Sequence:

ATGGAGGATC ACATGTTCGG TGTTCAGCAA ATCCAGCCCA ATGTCATTTC TGTTCGTCTC 
TTCAAGCGCA AAGTTGGGGG CCTGGGATTT CTGGTGAAGG AGCGGGTCAG TAAGCCGCCC 
GTGATCATCT CTGACCTGAT TCGTGGGGGC GCCGCAGAGC AGAGTGGCCT CATCCAGGCC 
GGAGACATCA TTCTTGCGGT CAACGGCCGG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC 
CTGGAGGTAC TCAGAGGCAT TGCCTCTGAG ACCCACGTGG TCCTCATTCT GAGGGGCCCT 
GAAGGTTTCA CCACGCACCT GGAGACCACC TTTACAGGTG ATGGGACCCC CAAGACCATC 
CGGGTGACAC AGCCCCTGGG TCCCCCCACC AAAGCCGTGG ATCTGTCCCA CCAGCCACCG 
GCCGGCAAAG AACAGCCCCT GGCAGTGGAT GGGGCCTCGG GTCCCGGGAA TGGGCCTCAG 
CATGCCTACG ATGATGGGCA GGAGGCTGGC TCACTCCCCC ATGCCAACGG CCTGGCCCCC 
AGGCCCCCAG GCCAGGACCC CGCGAAGAAA GCAACCAGAG TCAGCCTCCA AGGCAGAGGG 
GAGAACAATG AACTGCTCAA GGAGATAGAG CCTGTGCTGA GCCTTCTCAC CAGTGGGAGC 
AGAGGGGTCA AGGGAGGGGC ACCTGCCAAG GCAGAGATGA AAGATATGGG AATCCAGGTG 
GACAGAGATT TGGACGGCAA GTCACACAAA CCTCTGCCCC TCGGCGTGGA GAACGACCGA 
GTCTTCAATG ACCTATGGGG GAAGGGCAAT GTGCCTGTCG TCCTCAACAA CCCATATTCA 
GAGAAGGAGC AGCCCCCCAC CTCAGGAAAA CAGTCCCCCA CAAAGAATGG CAGCCCCTCC 
AAGTGTCCAC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG AGGTGGTTCT CACTGACACC 
CTCCACCTTA AGAGCACATT GGAAACGGGA TGCACTGAGT ACATCTGCAT GGGCTCCATC 
ATGCATCCTT CTCAGCATGC AAGGAGGCCT GAAGACGTCC GCACAAAAGG ACAGCTCTTC 
CCTCTCGCCA AAGAGTTTAT TGATCAATAC TATTCATCAA TTAAAAGATT TGGCTCCAAA 
GCCCACATGG AAAGGCTGGA AGAGGTGAAC AAAGAGATCG ACACCACTAG CACTTACCAG 
CTCAAGGACA CAGAGCTCAT CTATGGGGCC AAGCACGCCT GGCGGAATGC CTCGCGCTGT 
GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTATTCGATG CCCGTGACTG CACCACGGCC 
CACGGGATGT TCAACTACAT CTGTAACCAT GTCAAGTATG CCACCAACAA AGGGAACCTC 
AGGTCTGCCA TCACCATATT CCCCCAGAGG ACAGACGGCA AGCACGACTT CCGAGTCTGG 
AACTCCCAGC TCATCCGCTA CGCTGGCTAC AAGCAGCCTG ACGGCTCCAC CCTGGGGGAC 
CCAGCCAATG TGCAGTTCAC AGAGATATGC ATACAGCAGG GCTGGAAACC GCCTAGAGGC 
CGCTTCGATG TCCTGCCGCT CCTGCTTCAG GCCAACGGCA ATGACCCTGA GCTCTTCCAG 
ATTCCTCCAG AGCTGGTGTT GGAAGTTCCC ATCAGGCACC CCAAGTTTGA GTGGTTCAAG 
GACCTGGGGC TGAAGTGGTA CGGCCTCCCC GCCGTGTCCA ACATGCTCCT AGAGATTGGC 
GGCCTGGAGT TCAGCGCCTG TCCCTTCAGT GGCTGGTACA TGGGCACAGA GATTGGTGTC 
CGCGACTACT GTGACAACTC CCGCTACAAT ATCCTGGAGG AAGTGGCCAA GAAGATGAAC 
TTAGACATGA GGAAGACGTC CTCCCTGTGG AAGGACCAGG CGCTGGTGGA GATCAATATC 
GCGGTTCTCT ATAGCTTCCA GAGTGACAAA GTGACCATTG TTGACCATCA CTCCGCCACC 
GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGGGGCTG CCCTGCCGAC 
TGGGTGTGGA TCGTGCCCCC CATGTCCGGA AGCATCACCC CTGTGTTCCA CCAGGAGATG 
CTCAACTACC GGCTCACCCC CTCCTTCGAA TACCAGCCTG ATCCCTGGAA CACGCATGTC 
TGGAAAGGCA CCAACGGGAC CCCCACAAAG CGGCGAGCCA TTGGCTTCAA GAAGCTAGCA 
GAAGCTGTCA AGTTCTCGGC CAAGCTGATG GGGCAGGCTA TGGCCAAGAG GGTGAAAGCG 
ACCATCCTCT ATGCCACAGA GACAGGCAAA TCGCAAGCTT ATGCCAAGAC CTTGTGTGAG 
ATCTTCAAAC ACGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATTGTGCAC 
CTGGAACATG AAACTCTGGT CCTTGTGGTC ACCAGCACCT TTGGCAATGG AGATCCCCCT 
GAGAATGGGG AGAAATTCGG CTGTGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAG 
GAAGAAAGGA AGAGCTACAA GGTCCGATTC AACAGCGTCT CCTCCTACTC TGACTCCCAA 
AAATCATCAG GCGATGGGCC CGACCTCAGA GACAACTTTG AGAGTGCTGG ACCCCTGGCC 
AATGTGAGGT TCTCAGTTTT TGGCCTCGGC TCACGAGCAT ACCCTCACTT TTGCGCCTTC 
GGACACGCTG TGGACACCCT CCTGGAAGAA CTGGGAGGGG AGAGGATCCT GAAGATGAGG 
GAAGGGGATG AGCTCTGTGG GCAGGAAGAG GCTTTCAGGA CCTGGGCCAA GAAGGTCTTC 
AAGGCAGCCT GTGATGTCTT CTGTGTGGGA GATGATGTCA ACATTGAAAA GGCCAACAAT 
TCCCTCATCA GCAATGATCG CAGCTGGAAG AGAAACAAGT TCCGCCTCAC CTTTGTGGCC 
GAAGCTCCAG AACTCACACA AGGTCTATCC AATGTCCACA AAAAGCGAGT CTCAGCTGCC 
CGGCTCCTTA GCCGTCAAAA CCTCCAGAGC CCTAAATCCA GTCGGTCAAC TATCTTCGTG 
CGTCTCCACA CCAACGGGAG CCAGGAGCTG CAGTACCAGC CTGGGGACCA CCTGGGTGTC 
TTCCCTGGCA ACCACGAGGA CCTCGTGAAT GCCCTGATCG AGCGGCTGGA GGACGCGCCG 
CCTGTCAACC AGATGGTGAA AGTGGAACTG CTGGAGGAGC GGAACACGGC TTTAGGTGTC 
ATCAGTAACT GGACAGACGA GCTCCGCCTC CCGCCCTGCA CCATCTTCCA GGCCTTCAAG 
TACTACCTGG ACATCACCAC GCCACCAACG CCTCTGCAGC TGCAGCAGTT TGCCTCCCTA 
GCTACCAGCG AGAAGGAGAA GCAGCGTCTG CTGGTCCTCA GCAAGGGTTT GCAGGAGTAC 
GAGGAATGGA AATGGGGCAA GAACCCCACC ATCGTGGAGG TGCTGGAGGA GTTCCCATCT 
ATCCAGATGC CGGCCACCCT GCTCCTGACC CAGCTGTCCC TGCTGCAGCC CCGCTACTAT 
TCCATCAGCT CCTCCCCAGA CATGTACCCT GATGAAGTGC ACCTCACTGT GGCCATCGTT 
TCCTACCGCA CTCGAGATGG AGAAGGACCA ATTCACCACG GCGTATGCTC CTCCTGGCTC 
AACCGGATAC AGGCTGACGA ACTGGTCCCC TGTTTCGTGA GAGGAGCACC CAGCTTCCAC 
CTGCCCCGGA ACCCCCAAGT CCCCTGCATC CTCGTTGGAC CAGGCACCGG CATTGCCCCT 
TTCCGAAGCT TCTGGCAACA GCGGCAATTT GATATCCAAC ACAAAGGAAT GAACCCCTGC 
CCCATGGTCC TGGTCTTCGG GTGCCGGCAA TCCAAGATAG ATCATATCTA CAGGGAAGAG 
ACCCTGCAGG CCAAGAACAA GGGGGTCTTC AGAGAGCTGT ACACGGCTTA CTCCCGGGAG 
CCAGACAAAC CAAAGAAGTA CGTGCAGGAC ATCCTGCAGG AGCAGCTGGC GGAGTCTGTG 
TACCGAGCCC TGAAGGAGCA AGGGGGCCAC ATATACGTCT GTGGGGACGT CACCATGGCT 
GCTGATGTCC TCAAAGCCAT CCAGCGCATC ATGACCCAGC AGGGGAAGCT CTCGGCAGAG 
GACGCCGGCG TATTCATCAG CCGGATGAGG GATGACAACC GATACCATGA GGATATTTTT 
GGAGTCACCC TGCGAACGTA CGAAGTGACC AACCGCCTTA GATCTGAGTC CATTGCCTTC 
ATTGAAGAGA GCAAAAAAGA CACCGATGAG GTTTTCAGCT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOS1 ( NM_000620.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_000611.1 Homo sapiens nitric oxide synthase 1 (NOS1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu21020
Clone ID Related Accession (Same CDS sequence)	NM_000620.4 , NM_000620.5 , XM_017019347.1
Accession Version	NM_000620.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4305bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Homo sapiens(human)
Product	nitric oxide synthase, brain isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U17327.1, AK294435.1, AK307481.1, BC033208.1, AC026364.36 and BE207961.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_000620.4. Transcript Variant: This variant (1) represents the predominant transcript, and encodes isoform 1 (also known as nNOS). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU753241.1, U17327.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03465402, SAMN03465403 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000317775.11/ ENSP00000320758.6 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGGATC ACATGTTCGG TGTTCAGCAA ATCCAGCCCA ATGTCATTTC TGTTCGTCTC 
TTCAAGCGCA AAGTTGGGGG CCTGGGATTT CTGGTGAAGG AGCGGGTCAG TAAGCCGCCC 
GTGATCATCT CTGACCTGAT TCGTGGGGGC GCCGCAGAGC AGAGTGGCCT CATCCAGGCC 
GGAGACATCA TTCTTGCGGT CAACGGCCGG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC 
CTGGAGGTAC TCAGAGGCAT TGCCTCTGAG ACCCACGTGG TCCTCATTCT GAGGGGCCCT 
GAAGGTTTCA CCACGCACCT GGAGACCACC TTTACAGGTG ATGGGACCCC CAAGACCATC 
CGGGTGACAC AGCCCCTGGG TCCCCCCACC AAAGCCGTGG ATCTGTCCCA CCAGCCACCG 
GCCGGCAAAG AACAGCCCCT GGCAGTGGAT GGGGCCTCGG GTCCCGGGAA TGGGCCTCAG 
CATGCCTACG ATGATGGGCA GGAGGCTGGC TCACTCCCCC ATGCCAACGG CCTGGCCCCC 
AGGCCCCCAG GCCAGGACCC CGCGAAGAAA GCAACCAGAG TCAGCCTCCA AGGCAGAGGG 
GAGAACAATG AACTGCTCAA GGAGATAGAG CCTGTGCTGA GCCTTCTCAC CAGTGGGAGC 
AGAGGGGTCA AGGGAGGGGC ACCTGCCAAG GCAGAGATGA AAGATATGGG AATCCAGGTG 
GACAGAGATT TGGACGGCAA GTCACACAAA CCTCTGCCCC TCGGCGTGGA GAACGACCGA 
GTCTTCAATG ACCTATGGGG GAAGGGCAAT GTGCCTGTCG TCCTCAACAA CCCATATTCA 
GAGAAGGAGC AGCCCCCCAC CTCAGGAAAA CAGTCCCCCA CAAAGAATGG CAGCCCCTCC 
AAGTGTCCAC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG AGGTGGTTCT CACTGACACC 
CTCCACCTTA AGAGCACATT GGAAACGGGA TGCACTGAGT ACATCTGCAT GGGCTCCATC 
ATGCATCCTT CTCAGCATGC AAGGAGGCCT GAAGACGTCC GCACAAAAGG ACAGCTCTTC 
CCTCTCGCCA AAGAGTTTAT TGATCAATAC TATTCATCAA TTAAAAGATT TGGCTCCAAA 
GCCCACATGG AAAGGCTGGA AGAGGTGAAC AAAGAGATCG ACACCACTAG CACTTACCAG 
CTCAAGGACA CAGAGCTCAT CTATGGGGCC AAGCACGCCT GGCGGAATGC CTCGCGCTGT 
GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTATTCGATG CCCGTGACTG CACCACGGCC 
CACGGGATGT TCAACTACAT CTGTAACCAT GTCAAGTATG CCACCAACAA AGGGAACCTC 
AGGTCTGCCA TCACCATATT CCCCCAGAGG ACAGACGGCA AGCACGACTT CCGAGTCTGG 
AACTCCCAGC TCATCCGCTA CGCTGGCTAC AAGCAGCCTG ACGGCTCCAC CCTGGGGGAC 
CCAGCCAATG TGCAGTTCAC AGAGATATGC ATACAGCAGG GCTGGAAACC GCCTAGAGGC 
CGCTTCGATG TCCTGCCGCT CCTGCTTCAG GCCAACGGCA ATGACCCTGA GCTCTTCCAG 
ATTCCTCCAG AGCTGGTGTT GGAAGTTCCC ATCAGGCACC CCAAGTTTGA GTGGTTCAAG 
GACCTGGGGC TGAAGTGGTA CGGCCTCCCC GCCGTGTCCA ACATGCTCCT AGAGATTGGC 
GGCCTGGAGT TCAGCGCCTG TCCCTTCAGT GGCTGGTACA TGGGCACAGA GATTGGTGTC 
CGCGACTACT GTGACAACTC CCGCTACAAT ATCCTGGAGG AAGTGGCCAA GAAGATGAAC 
TTAGACATGA GGAAGACGTC CTCCCTGTGG AAGGACCAGG CGCTGGTGGA GATCAATATC 
GCGGTTCTCT ATAGCTTCCA GAGTGACAAA GTGACCATTG TTGACCATCA CTCCGCCACC 
GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGGGGCTG CCCTGCCGAC 
TGGGTGTGGA TCGTGCCCCC CATGTCCGGA AGCATCACCC CTGTGTTCCA CCAGGAGATG 
CTCAACTACC GGCTCACCCC CTCCTTCGAA TACCAGCCTG ATCCCTGGAA CACGCATGTC 
TGGAAAGGCA CCAACGGGAC CCCCACAAAG CGGCGAGCCA TTGGCTTCAA GAAGCTAGCA 
GAAGCTGTCA AGTTCTCGGC CAAGCTGATG GGGCAGGCTA TGGCCAAGAG GGTGAAAGCG 
ACCATCCTCT ATGCCACAGA GACAGGCAAA TCGCAAGCTT ATGCCAAGAC CTTGTGTGAG 
ATCTTCAAAC ACGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATTGTGCAC 
CTGGAACATG AAACTCTGGT CCTTGTGGTC ACCAGCACCT TTGGCAATGG AGATCCCCCT 
GAGAATGGGG AGAAATTCGG CTGTGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAG 
GAAGAAAGGA AGAGCTACAA GGTCCGATTC AACAGCGTCT CCTCCTACTC TGACTCCCAA 
AAATCATCAG GCGATGGGCC CGACCTCAGA GACAACTTTG AGAGTGCTGG ACCCCTGGCC 
AATGTGAGGT TCTCAGTTTT TGGCCTCGGC TCACGAGCAT ACCCTCACTT TTGCGCCTTC 
GGACACGCTG TGGACACCCT CCTGGAAGAA CTGGGAGGGG AGAGGATCCT GAAGATGAGG 
GAAGGGGATG AGCTCTGTGG GCAGGAAGAG GCTTTCAGGA CCTGGGCCAA GAAGGTCTTC 
AAGGCAGCCT GTGATGTCTT CTGTGTGGGA GATGATGTCA ACATTGAAAA GGCCAACAAT 
TCCCTCATCA GCAATGATCG CAGCTGGAAG AGAAACAAGT TCCGCCTCAC CTTTGTGGCC 
GAAGCTCCAG AACTCACACA AGGTCTATCC AATGTCCACA AAAAGCGAGT CTCAGCTGCC 
CGGCTCCTTA GCCGTCAAAA CCTCCAGAGC CCTAAATCCA GTCGGTCAAC TATCTTCGTG 
CGTCTCCACA CCAACGGGAG CCAGGAGCTG CAGTACCAGC CTGGGGACCA CCTGGGTGTC 
TTCCCTGGCA ACCACGAGGA CCTCGTGAAT GCCCTGATCG AGCGGCTGGA GGACGCGCCG 
CCTGTCAACC AGATGGTGAA AGTGGAACTG CTGGAGGAGC GGAACACGGC TTTAGGTGTC 
ATCAGTAACT GGACAGACGA GCTCCGCCTC CCGCCCTGCA CCATCTTCCA GGCCTTCAAG 
TACTACCTGG ACATCACCAC GCCACCAACG CCTCTGCAGC TGCAGCAGTT TGCCTCCCTA 
GCTACCAGCG AGAAGGAGAA GCAGCGTCTG CTGGTCCTCA GCAAGGGTTT GCAGGAGTAC 
GAGGAATGGA AATGGGGCAA GAACCCCACC ATCGTGGAGG TGCTGGAGGA GTTCCCATCT 
ATCCAGATGC CGGCCACCCT GCTCCTGACC CAGCTGTCCC TGCTGCAGCC CCGCTACTAT 
TCCATCAGCT CCTCCCCAGA CATGTACCCT GATGAAGTGC ACCTCACTGT GGCCATCGTT 
TCCTACCGCA CTCGAGATGG AGAAGGACCA ATTCACCACG GCGTATGCTC CTCCTGGCTC 
AACCGGATAC AGGCTGACGA ACTGGTCCCC TGTTTCGTGA GAGGAGCACC CAGCTTCCAC 
CTGCCCCGGA ACCCCCAAGT CCCCTGCATC CTCGTTGGAC CAGGCACCGG CATTGCCCCT 
TTCCGAAGCT TCTGGCAACA GCGGCAATTT GATATCCAAC ACAAAGGAAT GAACCCCTGC 
CCCATGGTCC TGGTCTTCGG GTGCCGGCAA TCCAAGATAG ATCATATCTA CAGGGAAGAG 
ACCCTGCAGG CCAAGAACAA GGGGGTCTTC AGAGAGCTGT ACACGGCTTA CTCCCGGGAG 
CCAGACAAAC CAAAGAAGTA CGTGCAGGAC ATCCTGCAGG AGCAGCTGGC GGAGTCTGTG 
TACCGAGCCC TGAAGGAGCA AGGGGGCCAC ATATACGTCT GTGGGGACGT CACCATGGCT 
GCTGATGTCC TCAAAGCCAT CCAGCGCATC ATGACCCAGC AGGGGAAGCT CTCGGCAGAG 
GACGCCGGCG TATTCATCAG CCGGATGAGG GATGACAACC GATACCATGA GGATATTTTT 
GGAGTCACCC TGCGAACGTA CGAAGTGACC AACCGCCTTA GATCTGAGTC CATTGCCTTC 
ATTGAAGAGA GCAAAAAAGA CACCGATGAG GTTTTCAGCT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000611.1
CDS	536..4840
Translation	MEDHMFGVQQIQPNVISVRLFKRKVGGLGFLVKERVSKPPVIISDLIRGGAAEQSGLIQAGDIILAVNGRPLVDLSYDSALEVLRGIASETHVVLILRGPEGFTTHLETTFTGDGTPKTIRVTQPLGPPTKAVDLSHQPPAGKEQPLAVDGASGPGNGPQHAYDDGQEAGSLPHANGLAPRPPGQDPAKKATRVSLQGRGENNELLKEIEPVLSLLTSGSRGVKGGAPAKAEMKDMGIQVDRDLDGKSHKPLPLGVENDRVFNDLWGKGNVPVVLNNPYSEKEQPPTSGKQSPTKNGSPSKCPRFLKVKNWETEVVLTDTLHLKSTLETGCTEYICMGSIMHPSQHARRPEDVRTKGQLFPLAKEFIDQYYSSIKRFGSKAHMERLEEVNKEIDTTSTYQLKDTELIYGAKHAWRNASRCVGRIQWSKLQVFDARDCTTAHGMFNYICNHVKYATNKGNLRSAITIFPQRTDGKHDFRVWNSQLIRYAGYKQPDGSTLGDPANVQFTEICIQQGWKPPRGRFDVLPLLLQANGNDPELFQIPPELVLEVPIRHPKFEWFKDLGLKWYGLPAVSNMLLEIGGLEFSACPFSGWYMGTEIGVRDYCDNSRYNILEEVAKKMNLDMRKTSSLWKDQALVEINIAVLYSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMLNYRLTPSFEYQPDPWNTHVWKGTNGTPTKRRAIGFKKLAEAVKFSAKLMGQAMAKRVKATILYATETGKSQAYAKTLCEIFKHAFDAKVMSMEEYDIVHLEHETLVLVVTSTFGNGDPPENGEKFGCALMEMRHPNSVQEERKSYKVRFNSVSSYSDSQKSSGDGPDLRDNFESAGPLANVRFSVFGLGSRAYPHFCAFGHAVDTLLEELGGERILKMREGDELCGQEEAFRTWAKKVFKAACDVFCVGDDVNIEKANNSLISNDRSWKRNKFRLTFVAEAPELTQGLSNVHKKRVSAARLLSRQNLQSPKSSRSTIFVRLHTNGSQELQYQPGDHLGVFPGNHEDLVNALIERLEDAPPVNQMVKVELLEERNTALGVISNWTDELRLPPCTIFQAFKYYLDITTPPTPLQLQQFASLATSEKEKQRLLVLSKGLQEYEEWKWGKNPTIVEVLEEFPSIQMPATLLLTQLSLLQPRYYSISSSPDMYPDEVHLTVAIVSYRTRDGEGPIHHGVCSSWLNRIQADELVPCFVRGAPSFHLPRNPQVPCILVGPGTGIAPFRSFWQQRQFDIQHKGMNPCPMVLVFGCRQSKIDHIYREETLQAKNKGVFRELYTAYSREPDKPKKYVQDILQEQLAESVYRALKEQGGHIYVCGDVTMAADVLKAIQRIMTQQGKLSAEDAGVFISRMRDDNRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDTDEVFSS

Target ORF information:

RefSeq Version	NM_000620.5
Organism	Homo sapiens(human)
Definition	Homo sapiens nitric oxide synthase 1 (NOS1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000620.5

ORF Insert Sequence:

ATGGAGGATC ACATGTTCGG TGTTCAGCAA ATCCAGCCCA ATGTCATTTC TGTTCGTCTC 
TTCAAGCGCA AAGTTGGGGG CCTGGGATTT CTGGTGAAGG AGCGGGTCAG TAAGCCGCCC 
GTGATCATCT CTGACCTGAT TCGTGGGGGC GCCGCAGAGC AGAGTGGCCT CATCCAGGCC 
GGAGACATCA TTCTTGCGGT CAACGGCCGG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC 
CTGGAGGTAC TCAGAGGCAT TGCCTCTGAG ACCCACGTGG TCCTCATTCT GAGGGGCCCT 
GAAGGTTTCA CCACGCACCT GGAGACCACC TTTACAGGTG ATGGGACCCC CAAGACCATC 
CGGGTGACAC AGCCCCTGGG TCCCCCCACC AAAGCCGTGG ATCTGTCCCA CCAGCCACCG 
GCCGGCAAAG AACAGCCCCT GGCAGTGGAT GGGGCCTCGG GTCCCGGGAA TGGGCCTCAG 
CATGCCTACG ATGATGGGCA GGAGGCTGGC TCACTCCCCC ATGCCAACGG CCTGGCCCCC 
AGGCCCCCAG GCCAGGACCC CGCGAAGAAA GCAACCAGAG TCAGCCTCCA AGGCAGAGGG 
GAGAACAATG AACTGCTCAA GGAGATAGAG CCTGTGCTGA GCCTTCTCAC CAGTGGGAGC 
AGAGGGGTCA AGGGAGGGGC ACCTGCCAAG GCAGAGATGA AAGATATGGG AATCCAGGTG 
GACAGAGATT TGGACGGCAA GTCACACAAA CCTCTGCCCC TCGGCGTGGA GAACGACCGA 
GTCTTCAATG ACCTATGGGG GAAGGGCAAT GTGCCTGTCG TCCTCAACAA CCCATATTCA 
GAGAAGGAGC AGCCCCCCAC CTCAGGAAAA CAGTCCCCCA CAAAGAATGG CAGCCCCTCC 
AAGTGTCCAC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG AGGTGGTTCT CACTGACACC 
CTCCACCTTA AGAGCACATT GGAAACGGGA TGCACTGAGT ACATCTGCAT GGGCTCCATC 
ATGCATCCTT CTCAGCATGC AAGGAGGCCT GAAGACGTCC GCACAAAAGG ACAGCTCTTC 
CCTCTCGCCA AAGAGTTTAT TGATCAATAC TATTCATCAA TTAAAAGATT TGGCTCCAAA 
GCCCACATGG AAAGGCTGGA AGAGGTGAAC AAAGAGATCG ACACCACTAG CACTTACCAG 
CTCAAGGACA CAGAGCTCAT CTATGGGGCC AAGCACGCCT GGCGGAATGC CTCGCGCTGT 
GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTATTCGATG CCCGTGACTG CACCACGGCC 
CACGGGATGT TCAACTACAT CTGTAACCAT GTCAAGTATG CCACCAACAA AGGGAACCTC 
AGGTCTGCCA TCACCATATT CCCCCAGAGG ACAGACGGCA AGCACGACTT CCGAGTCTGG 
AACTCCCAGC TCATCCGCTA CGCTGGCTAC AAGCAGCCTG ACGGCTCCAC CCTGGGGGAC 
CCAGCCAATG TGCAGTTCAC AGAGATATGC ATACAGCAGG GCTGGAAACC GCCTAGAGGC 
CGCTTCGATG TCCTGCCGCT CCTGCTTCAG GCCAACGGCA ATGACCCTGA GCTCTTCCAG 
ATTCCTCCAG AGCTGGTGTT GGAAGTTCCC ATCAGGCACC CCAAGTTTGA GTGGTTCAAG 
GACCTGGGGC TGAAGTGGTA CGGCCTCCCC GCCGTGTCCA ACATGCTCCT AGAGATTGGC 
GGCCTGGAGT TCAGCGCCTG TCCCTTCAGT GGCTGGTACA TGGGCACAGA GATTGGTGTC 
CGCGACTACT GTGACAACTC CCGCTACAAT ATCCTGGAGG AAGTGGCCAA GAAGATGAAC 
TTAGACATGA GGAAGACGTC CTCCCTGTGG AAGGACCAGG CGCTGGTGGA GATCAATATC 
GCGGTTCTCT ATAGCTTCCA GAGTGACAAA GTGACCATTG TTGACCATCA CTCCGCCACC 
GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGGGGCTG CCCTGCCGAC 
TGGGTGTGGA TCGTGCCCCC CATGTCCGGA AGCATCACCC CTGTGTTCCA CCAGGAGATG 
CTCAACTACC GGCTCACCCC CTCCTTCGAA TACCAGCCTG ATCCCTGGAA CACGCATGTC 
TGGAAAGGCA CCAACGGGAC CCCCACAAAG CGGCGAGCCA TTGGCTTCAA GAAGCTAGCA 
GAAGCTGTCA AGTTCTCGGC CAAGCTGATG GGGCAGGCTA TGGCCAAGAG GGTGAAAGCG 
ACCATCCTCT ATGCCACAGA GACAGGCAAA TCGCAAGCTT ATGCCAAGAC CTTGTGTGAG 
ATCTTCAAAC ACGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATTGTGCAC 
CTGGAACATG AAACTCTGGT CCTTGTGGTC ACCAGCACCT TTGGCAATGG AGATCCCCCT 
GAGAATGGGG AGAAATTCGG CTGTGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAG 
GAAGAAAGGA AGAGCTACAA GGTCCGATTC AACAGCGTCT CCTCCTACTC TGACTCCCAA 
AAATCATCAG GCGATGGGCC CGACCTCAGA GACAACTTTG AGAGTGCTGG ACCCCTGGCC 
AATGTGAGGT TCTCAGTTTT TGGCCTCGGC TCACGAGCAT ACCCTCACTT TTGCGCCTTC 
GGACACGCTG TGGACACCCT CCTGGAAGAA CTGGGAGGGG AGAGGATCCT GAAGATGAGG 
GAAGGGGATG AGCTCTGTGG GCAGGAAGAG GCTTTCAGGA CCTGGGCCAA GAAGGTCTTC 
AAGGCAGCCT GTGATGTCTT CTGTGTGGGA GATGATGTCA ACATTGAAAA GGCCAACAAT 
TCCCTCATCA GCAATGATCG CAGCTGGAAG AGAAACAAGT TCCGCCTCAC CTTTGTGGCC 
GAAGCTCCAG AACTCACACA AGGTCTATCC AATGTCCACA AAAAGCGAGT CTCAGCTGCC 
CGGCTCCTTA GCCGTCAAAA CCTCCAGAGC CCTAAATCCA GTCGGTCAAC TATCTTCGTG 
CGTCTCCACA CCAACGGGAG CCAGGAGCTG CAGTACCAGC CTGGGGACCA CCTGGGTGTC 
TTCCCTGGCA ACCACGAGGA CCTCGTGAAT GCCCTGATCG AGCGGCTGGA GGACGCGCCG 
CCTGTCAACC AGATGGTGAA AGTGGAACTG CTGGAGGAGC GGAACACGGC TTTAGGTGTC 
ATCAGTAACT GGACAGACGA GCTCCGCCTC CCGCCCTGCA CCATCTTCCA GGCCTTCAAG 
TACTACCTGG ACATCACCAC GCCACCAACG CCTCTGCAGC TGCAGCAGTT TGCCTCCCTA 
GCTACCAGCG AGAAGGAGAA GCAGCGTCTG CTGGTCCTCA GCAAGGGTTT GCAGGAGTAC 
GAGGAATGGA AATGGGGCAA GAACCCCACC ATCGTGGAGG TGCTGGAGGA GTTCCCATCT 
ATCCAGATGC CGGCCACCCT GCTCCTGACC CAGCTGTCCC TGCTGCAGCC CCGCTACTAT 
TCCATCAGCT CCTCCCCAGA CATGTACCCT GATGAAGTGC ACCTCACTGT GGCCATCGTT 
TCCTACCGCA CTCGAGATGG AGAAGGACCA ATTCACCACG GCGTATGCTC CTCCTGGCTC 
AACCGGATAC AGGCTGACGA ACTGGTCCCC TGTTTCGTGA GAGGAGCACC CAGCTTCCAC 
CTGCCCCGGA ACCCCCAAGT CCCCTGCATC CTCGTTGGAC CAGGCACCGG CATTGCCCCT 
TTCCGAAGCT TCTGGCAACA GCGGCAATTT GATATCCAAC ACAAAGGAAT GAACCCCTGC 
CCCATGGTCC TGGTCTTCGG GTGCCGGCAA TCCAAGATAG ATCATATCTA CAGGGAAGAG 
ACCCTGCAGG CCAAGAACAA GGGGGTCTTC AGAGAGCTGT ACACGGCTTA CTCCCGGGAG 
CCAGACAAAC CAAAGAAGTA CGTGCAGGAC ATCCTGCAGG AGCAGCTGGC GGAGTCTGTG 
TACCGAGCCC TGAAGGAGCA AGGGGGCCAC ATATACGTCT GTGGGGACGT CACCATGGCT 
GCTGATGTCC TCAAAGCCAT CCAGCGCATC ATGACCCAGC AGGGGAAGCT CTCGGCAGAG 
GACGCCGGCG TATTCATCAG CCGGATGAGG GATGACAACC GATACCATGA GGATATTTTT 
GGAGTCACCC TGCGAACGTA CGAAGTGACC AACCGCCTTA GATCTGAGTC CATTGCCTTC 
ATTGAAGAGA GCAAAAAAGA CACCGATGAG GTTTTCAGCT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOS1 ( NM_001204218.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001191147.1 Homo sapiens nitric oxide synthase 1 (NOS1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu21540
Clone ID Related Accession (Same CDS sequence)	XM_017019345.1 , XM_011538398.2 , XM_017019346.1 , NM_001204218.1
Accession Version	NM_001204218.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4407bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Homo sapiens(human)
Product	nitric oxide synthase, brain isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK307481.1, U17327.1, AK294435.1, AJ004918.1, BC033208.1, AC026364.36 and BE207961.1. Transcript Variant: This variant (2) contains an additional in-frame coding exon compared to variant 1, resulting in a longer isoform (2, also known as nNOSmu) containing a 34 aa protein segment not found in isoform 1. A similar isoform has also been reported in rat and mouse, and the mouse isoform has been shown to have catalytic activity (PMID:9791007). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGATC ACATGTTCGG TGTTCAGCAA ATCCAGCCCA ATGTCATTTC TGTTCGTCTC 
TTCAAGCGCA AAGTTGGGGG CCTGGGATTT CTGGTGAAGG AGCGGGTCAG TAAGCCGCCC 
GTGATCATCT CTGACCTGAT TCGTGGGGGC GCCGCAGAGC AGAGTGGCCT CATCCAGGCC 
GGAGACATCA TTCTTGCGGT CAACGGCCGG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC 
CTGGAGGTAC TCAGAGGCAT TGCCTCTGAG ACCCACGTGG TCCTCATTCT GAGGGGCCCT 
GAAGGTTTCA CCACGCACCT GGAGACCACC TTTACAGGTG ATGGGACCCC CAAGACCATC 
CGGGTGACAC AGCCCCTGGG TCCCCCCACC AAAGCCGTGG ATCTGTCCCA CCAGCCACCG 
GCCGGCAAAG AACAGCCCCT GGCAGTGGAT GGGGCCTCGG GTCCCGGGAA TGGGCCTCAG 
CATGCCTACG ATGATGGGCA GGAGGCTGGC TCACTCCCCC ATGCCAACGG CCTGGCCCCC 
AGGCCCCCAG GCCAGGACCC CGCGAAGAAA GCAACCAGAG TCAGCCTCCA AGGCAGAGGG 
GAGAACAATG AACTGCTCAA GGAGATAGAG CCTGTGCTGA GCCTTCTCAC CAGTGGGAGC 
AGAGGGGTCA AGGGAGGGGC ACCTGCCAAG GCAGAGATGA AAGATATGGG AATCCAGGTG 
GACAGAGATT TGGACGGCAA GTCACACAAA CCTCTGCCCC TCGGCGTGGA GAACGACCGA 
GTCTTCAATG ACCTATGGGG GAAGGGCAAT GTGCCTGTCG TCCTCAACAA CCCATATTCA 
GAGAAGGAGC AGCCCCCCAC CTCAGGAAAA CAGTCCCCCA CAAAGAATGG CAGCCCCTCC 
AAGTGTCCAC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG AGGTGGTTCT CACTGACACC 
CTCCACCTTA AGAGCACATT GGAAACGGGA TGCACTGAGT ACATCTGCAT GGGCTCCATC 
ATGCATCCTT CTCAGCATGC AAGGAGGCCT GAAGACGTCC GCACAAAAGG ACAGCTCTTC 
CCTCTCGCCA AAGAGTTTAT TGATCAATAC TATTCATCAA TTAAAAGATT TGGCTCCAAA 
GCCCACATGG AAAGGCTGGA AGAGGTGAAC AAAGAGATCG ACACCACTAG CACTTACCAG 
CTCAAGGACA CAGAGCTCAT CTATGGGGCC AAGCACGCCT GGCGGAATGC CTCGCGCTGT 
GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTATTCGATG CCCGTGACTG CACCACGGCC 
CACGGGATGT TCAACTACAT CTGTAACCAT GTCAAGTATG CCACCAACAA AGGGAACCTC 
AGGTCTGCCA TCACCATATT CCCCCAGAGG ACAGACGGCA AGCACGACTT CCGAGTCTGG 
AACTCCCAGC TCATCCGCTA CGCTGGCTAC AAGCAGCCTG ACGGCTCCAC CCTGGGGGAC 
CCAGCCAATG TGCAGTTCAC AGAGATATGC ATACAGCAGG GCTGGAAACC GCCTAGAGGC 
CGCTTCGATG TCCTGCCGCT CCTGCTTCAG GCCAACGGCA ATGACCCTGA GCTCTTCCAG 
ATTCCTCCAG AGCTGGTGTT GGAAGTTCCC ATCAGGCACC CCAAGTTTGA GTGGTTCAAG 
GACCTGGGGC TGAAGTGGTA CGGCCTCCCC GCCGTGTCCA ACATGCTCCT AGAGATTGGC 
GGCCTGGAGT TCAGCGCCTG TCCCTTCAGT GGCTGGTACA TGGGCACAGA GATTGGTGTC 
CGCGACTACT GTGACAACTC CCGCTACAAT ATCCTGGAGG AAGTGGCCAA GAAGATGAAC 
TTAGACATGA GGAAGACGTC CTCCCTGTGG AAGGACCAGG CGCTGGTGGA GATCAATATC 
GCGGTTCTCT ATAGCTTCCA GAGTGACAAA GTGACCATTG TTGACCATCA CTCCGCCACC 
GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGGGGCTG CCCTGCCGAC 
TGGGTGTGGA TCGTGCCCCC CATGTCCGGA AGCATCACCC CTGTGTTCCA CCAGGAGATG 
CTCAACTACC GGCTCACCCC CTCCTTCGAA TACCAGCCTG ATCCCTGGAA CACGCATGTC 
TGGAAAGGCA CCAACGGGAC CCCCACAAAG CGGCGAGCCA TTGGCTTCAA GAAGCTAGCA 
GAAGCTGTCA AGTTCTCGGC CAAGCTGATG GGGCAGGCTA TGGCCAAGAG GGTGAAAGCG 
ACCATCCTCT ATGCCACAGA GACAGGCAAA TCGCAAGCTT ATGCCAAGAC CTTGTGTGAG 
ATCTTCAAAC ACGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATTGTGCAC 
CTGGAACATG AAACTCTGGT CCTTGTGGTC ACCAGCACCT TTGGCAATGG AGATCCCCCT 
GAGAATGGGG AGAAATTCGG CTGTGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAG 
GAAGAAAGGA AGTACCCGGA ACCCTTGCGT TTCTTTCCCC GTAAAGGGCC TCCCCTCCCC 
AATGGTGACA CAGAAGTCCA CGGTCTGGCT GCAGCCCGTG ACAGCCAGCA CAGGAGCTAC 
AAGGTCCGAT TCAACAGCGT CTCCTCCTAC TCTGACTCCC AAAAATCATC AGGCGATGGG 
CCCGACCTCA GAGACAACTT TGAGAGTGCT GGACCCCTGG CCAATGTGAG GTTCTCAGTT 
TTTGGCCTCG GCTCACGAGC ATACCCTCAC TTTTGCGCCT TCGGACACGC TGTGGACACC 
CTCCTGGAAG AACTGGGAGG GGAGAGGATC CTGAAGATGA GGGAAGGGGA TGAGCTCTGT 
GGGCAGGAAG AGGCTTTCAG GACCTGGGCC AAGAAGGTCT TCAAGGCAGC CTGTGATGTC 
TTCTGTGTGG GAGATGATGT CAACATTGAA AAGGCCAACA ATTCCCTCAT CAGCAATGAT 
CGCAGCTGGA AGAGAAACAA GTTCCGCCTC ACCTTTGTGG CCGAAGCTCC AGAACTCACA 
CAAGGTCTAT CCAATGTCCA CAAAAAGCGA GTCTCAGCTG CCCGGCTCCT TAGCCGTCAA 
AACCTCCAGA GCCCTAAATC CAGTCGGTCA ACTATCTTCG TGCGTCTCCA CACCAACGGG 
AGCCAGGAGC TGCAGTACCA GCCTGGGGAC CACCTGGGTG TCTTCCCTGG CAACCACGAG 
GACCTCGTGA ATGCCCTGAT CGAGCGGCTG GAGGACGCGC CGCCTGTCAA CCAGATGGTG 
AAAGTGGAAC TGCTGGAGGA GCGGAACACG GCTTTAGGTG TCATCAGTAA CTGGACAGAC 
GAGCTCCGCC TCCCGCCCTG CACCATCTTC CAGGCCTTCA AGTACTACCT GGACATCACC 
ACGCCACCAA CGCCTCTGCA GCTGCAGCAG TTTGCCTCCC TAGCTACCAG CGAGAAGGAG 
AAGCAGCGTC TGCTGGTCCT CAGCAAGGGT TTGCAGGAGT ACGAGGAATG GAAATGGGGC 
AAGAACCCCA CCATCGTGGA GGTGCTGGAG GAGTTCCCAT CTATCCAGAT GCCGGCCACC 
CTGCTCCTGA CCCAGCTGTC CCTGCTGCAG CCCCGCTACT ATTCCATCAG CTCCTCCCCA 
GACATGTACC CTGATGAAGT GCACCTCACT GTGGCCATCG TTTCCTACCG CACTCGAGAT 
GGAGAAGGAC CAATTCACCA CGGCGTATGC TCCTCCTGGC TCAACCGGAT ACAGGCTGAC 
GAACTGGTCC CCTGTTTCGT GAGAGGAGCA CCCAGCTTCC ACCTGCCCCG GAACCCCCAA 
GTCCCCTGCA TCCTCGTTGG ACCAGGCACC GGCATTGCCC CTTTCCGAAG CTTCTGGCAA 
CAGCGGCAAT TTGATATCCA ACACAAAGGA ATGAACCCCT GCCCCATGGT CCTGGTCTTC 
GGGTGCCGGC AATCCAAGAT AGATCATATC TACAGGGAAG AGACCCTGCA GGCCAAGAAC 
AAGGGGGTCT TCAGAGAGCT GTACACGGCT TACTCCCGGG AGCCAGACAA ACCAAAGAAG 
TACGTGCAGG ACATCCTGCA GGAGCAGCTG GCGGAGTCTG TGTACCGAGC CCTGAAGGAG 
CAAGGGGGCC ACATATACGT CTGTGGGGAC GTCACCATGG CTGCTGATGT CCTCAAAGCC 
ATCCAGCGCA TCATGACCCA GCAGGGGAAG CTCTCGGCAG AGGACGCCGG CGTATTCATC 
AGCCGGATGA GGGATGACAA CCGATACCAT GAGGATATTT TTGGAGTCAC CCTGCGAACG 
TACGAAGTGA CCAACCGCCT TAGATCTGAG TCCATTGCCT TCATTGAAGA GAGCAAAAAA 
GACACCGATG AGGTTTTCAG CTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001191147.1
CDS	712..5118
Translation	MEDHMFGVQQIQPNVISVRLFKRKVGGLGFLVKERVSKPPVIISDLIRGGAAEQSGLIQAGDIILAVNGRPLVDLSYDSALEVLRGIASETHVVLILRGPEGFTTHLETTFTGDGTPKTIRVTQPLGPPTKAVDLSHQPPAGKEQPLAVDGASGPGNGPQHAYDDGQEAGSLPHANGLAPRPPGQDPAKKATRVSLQGRGENNELLKEIEPVLSLLTSGSRGVKGGAPAKAEMKDMGIQVDRDLDGKSHKPLPLGVENDRVFNDLWGKGNVPVVLNNPYSEKEQPPTSGKQSPTKNGSPSKCPRFLKVKNWETEVVLTDTLHLKSTLETGCTEYICMGSIMHPSQHARRPEDVRTKGQLFPLAKEFIDQYYSSIKRFGSKAHMERLEEVNKEIDTTSTYQLKDTELIYGAKHAWRNASRCVGRIQWSKLQVFDARDCTTAHGMFNYICNHVKYATNKGNLRSAITIFPQRTDGKHDFRVWNSQLIRYAGYKQPDGSTLGDPANVQFTEICIQQGWKPPRGRFDVLPLLLQANGNDPELFQIPPELVLEVPIRHPKFEWFKDLGLKWYGLPAVSNMLLEIGGLEFSACPFSGWYMGTEIGVRDYCDNSRYNILEEVAKKMNLDMRKTSSLWKDQALVEINIAVLYSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMLNYRLTPSFEYQPDPWNTHVWKGTNGTPTKRRAIGFKKLAEAVKFSAKLMGQAMAKRVKATILYATETGKSQAYAKTLCEIFKHAFDAKVMSMEEYDIVHLEHETLVLVVTSTFGNGDPPENGEKFGCALMEMRHPNSVQEERKYPEPLRFFPRKGPPLPNGDTEVHGLAAARDSQHRSYKVRFNSVSSYSDSQKSSGDGPDLRDNFESAGPLANVRFSVFGLGSRAYPHFCAFGHAVDTLLEELGGERILKMREGDELCGQEEAFRTWAKKVFKAACDVFCVGDDVNIEKANNSLISNDRSWKRNKFRLTFVAEAPELTQGLSNVHKKRVSAARLLSRQNLQSPKSSRSTIFVRLHTNGSQELQYQPGDHLGVFPGNHEDLVNALIERLEDAPPVNQMVKVELLEERNTALGVISNWTDELRLPPCTIFQAFKYYLDITTPPTPLQLQQFASLATSEKEKQRLLVLSKGLQEYEEWKWGKNPTIVEVLEEFPSIQMPATLLLTQLSLLQPRYYSISSSPDMYPDEVHLTVAIVSYRTRDGEGPIHHGVCSSWLNRIQADELVPCFVRGAPSFHLPRNPQVPCILVGPGTGIAPFRSFWQQRQFDIQHKGMNPCPMVLVFGCRQSKIDHIYREETLQAKNKGVFRELYTAYSREPDKPKKYVQDILQEQLAESVYRALKEQGGHIYVCGDVTMAADVLKAIQRIMTQQGKLSAEDAGVFISRMRDDNRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDTDEVFSS

Target ORF information:

RefSeq Version	NM_001204218.1
Organism	Homo sapiens(human)
Definition	Homo sapiens nitric oxide synthase 1 (NOS1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001204218.1

ORF Insert Sequence:

ATGGAGGATC ACATGTTCGG TGTTCAGCAA ATCCAGCCCA ATGTCATTTC TGTTCGTCTC 
TTCAAGCGCA AAGTTGGGGG CCTGGGATTT CTGGTGAAGG AGCGGGTCAG TAAGCCGCCC 
GTGATCATCT CTGACCTGAT TCGTGGGGGC GCCGCAGAGC AGAGTGGCCT CATCCAGGCC 
GGAGACATCA TTCTTGCGGT CAACGGCCGG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC 
CTGGAGGTAC TCAGAGGCAT TGCCTCTGAG ACCCACGTGG TCCTCATTCT GAGGGGCCCT 
GAAGGTTTCA CCACGCACCT GGAGACCACC TTTACAGGTG ATGGGACCCC CAAGACCATC 
CGGGTGACAC AGCCCCTGGG TCCCCCCACC AAAGCCGTGG ATCTGTCCCA CCAGCCACCG 
GCCGGCAAAG AACAGCCCCT GGCAGTGGAT GGGGCCTCGG GTCCCGGGAA TGGGCCTCAG 
CATGCCTACG ATGATGGGCA GGAGGCTGGC TCACTCCCCC ATGCCAACGG CCTGGCCCCC 
AGGCCCCCAG GCCAGGACCC CGCGAAGAAA GCAACCAGAG TCAGCCTCCA AGGCAGAGGG 
GAGAACAATG AACTGCTCAA GGAGATAGAG CCTGTGCTGA GCCTTCTCAC CAGTGGGAGC 
AGAGGGGTCA AGGGAGGGGC ACCTGCCAAG GCAGAGATGA AAGATATGGG AATCCAGGTG 
GACAGAGATT TGGACGGCAA GTCACACAAA CCTCTGCCCC TCGGCGTGGA GAACGACCGA 
GTCTTCAATG ACCTATGGGG GAAGGGCAAT GTGCCTGTCG TCCTCAACAA CCCATATTCA 
GAGAAGGAGC AGCCCCCCAC CTCAGGAAAA CAGTCCCCCA CAAAGAATGG CAGCCCCTCC 
AAGTGTCCAC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG AGGTGGTTCT CACTGACACC 
CTCCACCTTA AGAGCACATT GGAAACGGGA TGCACTGAGT ACATCTGCAT GGGCTCCATC 
ATGCATCCTT CTCAGCATGC AAGGAGGCCT GAAGACGTCC GCACAAAAGG ACAGCTCTTC 
CCTCTCGCCA AAGAGTTTAT TGATCAATAC TATTCATCAA TTAAAAGATT TGGCTCCAAA 
GCCCACATGG AAAGGCTGGA AGAGGTGAAC AAAGAGATCG ACACCACTAG CACTTACCAG 
CTCAAGGACA CAGAGCTCAT CTATGGGGCC AAGCACGCCT GGCGGAATGC CTCGCGCTGT 
GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTATTCGATG CCCGTGACTG CACCACGGCC 
CACGGGATGT TCAACTACAT CTGTAACCAT GTCAAGTATG CCACCAACAA AGGGAACCTC 
AGGTCTGCCA TCACCATATT CCCCCAGAGG ACAGACGGCA AGCACGACTT CCGAGTCTGG 
AACTCCCAGC TCATCCGCTA CGCTGGCTAC AAGCAGCCTG ACGGCTCCAC CCTGGGGGAC 
CCAGCCAATG TGCAGTTCAC AGAGATATGC ATACAGCAGG GCTGGAAACC GCCTAGAGGC 
CGCTTCGATG TCCTGCCGCT CCTGCTTCAG GCCAACGGCA ATGACCCTGA GCTCTTCCAG 
ATTCCTCCAG AGCTGGTGTT GGAAGTTCCC ATCAGGCACC CCAAGTTTGA GTGGTTCAAG 
GACCTGGGGC TGAAGTGGTA CGGCCTCCCC GCCGTGTCCA ACATGCTCCT AGAGATTGGC 
GGCCTGGAGT TCAGCGCCTG TCCCTTCAGT GGCTGGTACA TGGGCACAGA GATTGGTGTC 
CGCGACTACT GTGACAACTC CCGCTACAAT ATCCTGGAGG AAGTGGCCAA GAAGATGAAC 
TTAGACATGA GGAAGACGTC CTCCCTGTGG AAGGACCAGG CGCTGGTGGA GATCAATATC 
GCGGTTCTCT ATAGCTTCCA GAGTGACAAA GTGACCATTG TTGACCATCA CTCCGCCACC 
GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGGGGCTG CCCTGCCGAC 
TGGGTGTGGA TCGTGCCCCC CATGTCCGGA AGCATCACCC CTGTGTTCCA CCAGGAGATG 
CTCAACTACC GGCTCACCCC CTCCTTCGAA TACCAGCCTG ATCCCTGGAA CACGCATGTC 
TGGAAAGGCA CCAACGGGAC CCCCACAAAG CGGCGAGCCA TTGGCTTCAA GAAGCTAGCA 
GAAGCTGTCA AGTTCTCGGC CAAGCTGATG GGGCAGGCTA TGGCCAAGAG GGTGAAAGCG 
ACCATCCTCT ATGCCACAGA GACAGGCAAA TCGCAAGCTT ATGCCAAGAC CTTGTGTGAG 
ATCTTCAAAC ACGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATTGTGCAC 
CTGGAACATG AAACTCTGGT CCTTGTGGTC ACCAGCACCT TTGGCAATGG AGATCCCCCT 
GAGAATGGGG AGAAATTCGG CTGTGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAG 
GAAGAAAGGA AGTACCCGGA ACCCTTGCGT TTCTTTCCCC GTAAAGGGCC TCCCCTCCCC 
AATGGTGACA CAGAAGTCCA CGGTCTGGCT GCAGCCCGTG ACAGCCAGCA CAGGAGCTAC 
AAGGTCCGAT TCAACAGCGT CTCCTCCTAC TCTGACTCCC AAAAATCATC AGGCGATGGG 
CCCGACCTCA GAGACAACTT TGAGAGTGCT GGACCCCTGG CCAATGTGAG GTTCTCAGTT 
TTTGGCCTCG GCTCACGAGC ATACCCTCAC TTTTGCGCCT TCGGACACGC TGTGGACACC 
CTCCTGGAAG AACTGGGAGG GGAGAGGATC CTGAAGATGA GGGAAGGGGA TGAGCTCTGT 
GGGCAGGAAG AGGCTTTCAG GACCTGGGCC AAGAAGGTCT TCAAGGCAGC CTGTGATGTC 
TTCTGTGTGG GAGATGATGT CAACATTGAA AAGGCCAACA ATTCCCTCAT CAGCAATGAT 
CGCAGCTGGA AGAGAAACAA GTTCCGCCTC ACCTTTGTGG CCGAAGCTCC AGAACTCACA 
CAAGGTCTAT CCAATGTCCA CAAAAAGCGA GTCTCAGCTG CCCGGCTCCT TAGCCGTCAA 
AACCTCCAGA GCCCTAAATC CAGTCGGTCA ACTATCTTCG TGCGTCTCCA CACCAACGGG 
AGCCAGGAGC TGCAGTACCA GCCTGGGGAC CACCTGGGTG TCTTCCCTGG CAACCACGAG 
GACCTCGTGA ATGCCCTGAT CGAGCGGCTG GAGGACGCGC CGCCTGTCAA CCAGATGGTG 
AAAGTGGAAC TGCTGGAGGA GCGGAACACG GCTTTAGGTG TCATCAGTAA CTGGACAGAC 
GAGCTCCGCC TCCCGCCCTG CACCATCTTC CAGGCCTTCA AGTACTACCT GGACATCACC 
ACGCCACCAA CGCCTCTGCA GCTGCAGCAG TTTGCCTCCC TAGCTACCAG CGAGAAGGAG 
AAGCAGCGTC TGCTGGTCCT CAGCAAGGGT TTGCAGGAGT ACGAGGAATG GAAATGGGGC 
AAGAACCCCA CCATCGTGGA GGTGCTGGAG GAGTTCCCAT CTATCCAGAT GCCGGCCACC 
CTGCTCCTGA CCCAGCTGTC CCTGCTGCAG CCCCGCTACT ATTCCATCAG CTCCTCCCCA 
GACATGTACC CTGATGAAGT GCACCTCACT GTGGCCATCG TTTCCTACCG CACTCGAGAT 
GGAGAAGGAC CAATTCACCA CGGCGTATGC TCCTCCTGGC TCAACCGGAT ACAGGCTGAC 
GAACTGGTCC CCTGTTTCGT GAGAGGAGCA CCCAGCTTCC ACCTGCCCCG GAACCCCCAA 
GTCCCCTGCA TCCTCGTTGG ACCAGGCACC GGCATTGCCC CTTTCCGAAG CTTCTGGCAA 
CAGCGGCAAT TTGATATCCA ACACAAAGGA ATGAACCCCT GCCCCATGGT CCTGGTCTTC 
GGGTGCCGGC AATCCAAGAT AGATCATATC TACAGGGAAG AGACCCTGCA GGCCAAGAAC 
AAGGGGGTCT TCAGAGAGCT GTACACGGCT TACTCCCGGG AGCCAGACAA ACCAAAGAAG 
TACGTGCAGG ACATCCTGCA GGAGCAGCTG GCGGAGTCTG TGTACCGAGC CCTGAAGGAG 
CAAGGGGGCC ACATATACGT CTGTGGGGAC GTCACCATGG CTGCTGATGT CCTCAAAGCC 
ATCCAGCGCA TCATGACCCA GCAGGGGAAG CTCTCGGCAG AGGACGCCGG CGTATTCATC 
AGCCGGATGA GGGATGACAA CCGATACCAT GAGGATATTT TTGGAGTCAC CCTGCGAACG 
TACGAAGTGA CCAACCGCCT TAGATCTGAG TCCATTGCCT TCATTGAAGA GAGCAAAAAA 
GACACCGATG AGGTTTTCAG CTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOS1 ( XM_017019347.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016874836.1 Homo sapiens nitric oxide synthase 1 (NOS1), transcript variant X4, mRNA.

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CloneID	OHu21020
Clone ID Related Accession (Same CDS sequence)	NM_000620.4 , NM_000620.5 , XM_017019347.1
Accession Version	XM_017019347.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4305bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	nitric oxide synthase, brain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGATC ACATGTTCGG TGTTCAGCAA ATCCAGCCCA ATGTCATTTC TGTTCGTCTC 
TTCAAGCGCA AAGTTGGGGG CCTGGGATTT CTGGTGAAGG AGCGGGTCAG TAAGCCGCCC 
GTGATCATCT CTGACCTGAT TCGTGGGGGC GCCGCAGAGC AGAGTGGCCT CATCCAGGCC 
GGAGACATCA TTCTTGCGGT CAACGGCCGG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC 
CTGGAGGTAC TCAGAGGCAT TGCCTCTGAG ACCCACGTGG TCCTCATTCT GAGGGGCCCT 
GAAGGTTTCA CCACGCACCT GGAGACCACC TTTACAGGTG ATGGGACCCC CAAGACCATC 
CGGGTGACAC AGCCCCTGGG TCCCCCCACC AAAGCCGTGG ATCTGTCCCA CCAGCCACCG 
GCCGGCAAAG AACAGCCCCT GGCAGTGGAT GGGGCCTCGG GTCCCGGGAA TGGGCCTCAG 
CATGCCTACG ATGATGGGCA GGAGGCTGGC TCACTCCCCC ATGCCAACGG CCTGGCCCCC 
AGGCCCCCAG GCCAGGACCC CGCGAAGAAA GCAACCAGAG TCAGCCTCCA AGGCAGAGGG 
GAGAACAATG AACTGCTCAA GGAGATAGAG CCTGTGCTGA GCCTTCTCAC CAGTGGGAGC 
AGAGGGGTCA AGGGAGGGGC ACCTGCCAAG GCAGAGATGA AAGATATGGG AATCCAGGTG 
GACAGAGATT TGGACGGCAA GTCACACAAA CCTCTGCCCC TCGGCGTGGA GAACGACCGA 
GTCTTCAATG ACCTATGGGG GAAGGGCAAT GTGCCTGTCG TCCTCAACAA CCCATATTCA 
GAGAAGGAGC AGCCCCCCAC CTCAGGAAAA CAGTCCCCCA CAAAGAATGG CAGCCCCTCC 
AAGTGTCCAC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG AGGTGGTTCT CACTGACACC 
CTCCACCTTA AGAGCACATT GGAAACGGGA TGCACTGAGT ACATCTGCAT GGGCTCCATC 
ATGCATCCTT CTCAGCATGC AAGGAGGCCT GAAGACGTCC GCACAAAAGG ACAGCTCTTC 
CCTCTCGCCA AAGAGTTTAT TGATCAATAC TATTCATCAA TTAAAAGATT TGGCTCCAAA 
GCCCACATGG AAAGGCTGGA AGAGGTGAAC AAAGAGATCG ACACCACTAG CACTTACCAG 
CTCAAGGACA CAGAGCTCAT CTATGGGGCC AAGCACGCCT GGCGGAATGC CTCGCGCTGT 
GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTATTCGATG CCCGTGACTG CACCACGGCC 
CACGGGATGT TCAACTACAT CTGTAACCAT GTCAAGTATG CCACCAACAA AGGGAACCTC 
AGGTCTGCCA TCACCATATT CCCCCAGAGG ACAGACGGCA AGCACGACTT CCGAGTCTGG 
AACTCCCAGC TCATCCGCTA CGCTGGCTAC AAGCAGCCTG ACGGCTCCAC CCTGGGGGAC 
CCAGCCAATG TGCAGTTCAC AGAGATATGC ATACAGCAGG GCTGGAAACC GCCTAGAGGC 
CGCTTCGATG TCCTGCCGCT CCTGCTTCAG GCCAACGGCA ATGACCCTGA GCTCTTCCAG 
ATTCCTCCAG AGCTGGTGTT GGAAGTTCCC ATCAGGCACC CCAAGTTTGA GTGGTTCAAG 
GACCTGGGGC TGAAGTGGTA CGGCCTCCCC GCCGTGTCCA ACATGCTCCT AGAGATTGGC 
GGCCTGGAGT TCAGCGCCTG TCCCTTCAGT GGCTGGTACA TGGGCACAGA GATTGGTGTC 
CGCGACTACT GTGACAACTC CCGCTACAAT ATCCTGGAGG AAGTGGCCAA GAAGATGAAC 
TTAGACATGA GGAAGACGTC CTCCCTGTGG AAGGACCAGG CGCTGGTGGA GATCAATATC 
GCGGTTCTCT ATAGCTTCCA GAGTGACAAA GTGACCATTG TTGACCATCA CTCCGCCACC 
GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGGGGCTG CCCTGCCGAC 
TGGGTGTGGA TCGTGCCCCC CATGTCCGGA AGCATCACCC CTGTGTTCCA CCAGGAGATG 
CTCAACTACC GGCTCACCCC CTCCTTCGAA TACCAGCCTG ATCCCTGGAA CACGCATGTC 
TGGAAAGGCA CCAACGGGAC CCCCACAAAG CGGCGAGCCA TTGGCTTCAA GAAGCTAGCA 
GAAGCTGTCA AGTTCTCGGC CAAGCTGATG GGGCAGGCTA TGGCCAAGAG GGTGAAAGCG 
ACCATCCTCT ATGCCACAGA GACAGGCAAA TCGCAAGCTT ATGCCAAGAC CTTGTGTGAG 
ATCTTCAAAC ACGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATTGTGCAC 
CTGGAACATG AAACTCTGGT CCTTGTGGTC ACCAGCACCT TTGGCAATGG AGATCCCCCT 
GAGAATGGGG AGAAATTCGG CTGTGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAG 
GAAGAAAGGA AGAGCTACAA GGTCCGATTC AACAGCGTCT CCTCCTACTC TGACTCCCAA 
AAATCATCAG GCGATGGGCC CGACCTCAGA GACAACTTTG AGAGTGCTGG ACCCCTGGCC 
AATGTGAGGT TCTCAGTTTT TGGCCTCGGC TCACGAGCAT ACCCTCACTT TTGCGCCTTC 
GGACACGCTG TGGACACCCT CCTGGAAGAA CTGGGAGGGG AGAGGATCCT GAAGATGAGG 
GAAGGGGATG AGCTCTGTGG GCAGGAAGAG GCTTTCAGGA CCTGGGCCAA GAAGGTCTTC 
AAGGCAGCCT GTGATGTCTT CTGTGTGGGA GATGATGTCA ACATTGAAAA GGCCAACAAT 
TCCCTCATCA GCAATGATCG CAGCTGGAAG AGAAACAAGT TCCGCCTCAC CTTTGTGGCC 
GAAGCTCCAG AACTCACACA AGGTCTATCC AATGTCCACA AAAAGCGAGT CTCAGCTGCC 
CGGCTCCTTA GCCGTCAAAA CCTCCAGAGC CCTAAATCCA GTCGGTCAAC TATCTTCGTG 
CGTCTCCACA CCAACGGGAG CCAGGAGCTG CAGTACCAGC CTGGGGACCA CCTGGGTGTC 
TTCCCTGGCA ACCACGAGGA CCTCGTGAAT GCCCTGATCG AGCGGCTGGA GGACGCGCCG 
CCTGTCAACC AGATGGTGAA AGTGGAACTG CTGGAGGAGC GGAACACGGC TTTAGGTGTC 
ATCAGTAACT GGACAGACGA GCTCCGCCTC CCGCCCTGCA CCATCTTCCA GGCCTTCAAG 
TACTACCTGG ACATCACCAC GCCACCAACG CCTCTGCAGC TGCAGCAGTT TGCCTCCCTA 
GCTACCAGCG AGAAGGAGAA GCAGCGTCTG CTGGTCCTCA GCAAGGGTTT GCAGGAGTAC 
GAGGAATGGA AATGGGGCAA GAACCCCACC ATCGTGGAGG TGCTGGAGGA GTTCCCATCT 
ATCCAGATGC CGGCCACCCT GCTCCTGACC CAGCTGTCCC TGCTGCAGCC CCGCTACTAT 
TCCATCAGCT CCTCCCCAGA CATGTACCCT GATGAAGTGC ACCTCACTGT GGCCATCGTT 
TCCTACCGCA CTCGAGATGG AGAAGGACCA ATTCACCACG GCGTATGCTC CTCCTGGCTC 
AACCGGATAC AGGCTGACGA ACTGGTCCCC TGTTTCGTGA GAGGAGCACC CAGCTTCCAC 
CTGCCCCGGA ACCCCCAAGT CCCCTGCATC CTCGTTGGAC CAGGCACCGG CATTGCCCCT 
TTCCGAAGCT TCTGGCAACA GCGGCAATTT GATATCCAAC ACAAAGGAAT GAACCCCTGC 
CCCATGGTCC TGGTCTTCGG GTGCCGGCAA TCCAAGATAG ATCATATCTA CAGGGAAGAG 
ACCCTGCAGG CCAAGAACAA GGGGGTCTTC AGAGAGCTGT ACACGGCTTA CTCCCGGGAG 
CCAGACAAAC CAAAGAAGTA CGTGCAGGAC ATCCTGCAGG AGCAGCTGGC GGAGTCTGTG 
TACCGAGCCC TGAAGGAGCA AGGGGGCCAC ATATACGTCT GTGGGGACGT CACCATGGCT 
GCTGATGTCC TCAAAGCCAT CCAGCGCATC ATGACCCAGC AGGGGAAGCT CTCGGCAGAG 
GACGCCGGCG TATTCATCAG CCGGATGAGG GATGACAACC GATACCATGA GGATATTTTT 
GGAGTCACCC TGCGAACGTA CGAAGTGACC AACCGCCTTA GATCTGAGTC CATTGCCTTC 
ATTGAAGAGA GCAAAAAAGA CACCGATGAG GTTTTCAGCT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016874836.1
CDS	622..4926
Translation	MEDHMFGVQQIQPNVISVRLFKRKVGGLGFLVKERVSKPPVIISDLIRGGAAEQSGLIQAGDIILAVNGRPLVDLSYDSALEVLRGIASETHVVLILRGPEGFTTHLETTFTGDGTPKTIRVTQPLGPPTKAVDLSHQPPAGKEQPLAVDGASGPGNGPQHAYDDGQEAGSLPHANGLAPRPPGQDPAKKATRVSLQGRGENNELLKEIEPVLSLLTSGSRGVKGGAPAKAEMKDMGIQVDRDLDGKSHKPLPLGVENDRVFNDLWGKGNVPVVLNNPYSEKEQPPTSGKQSPTKNGSPSKCPRFLKVKNWETEVVLTDTLHLKSTLETGCTEYICMGSIMHPSQHARRPEDVRTKGQLFPLAKEFIDQYYSSIKRFGSKAHMERLEEVNKEIDTTSTYQLKDTELIYGAKHAWRNASRCVGRIQWSKLQVFDARDCTTAHGMFNYICNHVKYATNKGNLRSAITIFPQRTDGKHDFRVWNSQLIRYAGYKQPDGSTLGDPANVQFTEICIQQGWKPPRGRFDVLPLLLQANGNDPELFQIPPELVLEVPIRHPKFEWFKDLGLKWYGLPAVSNMLLEIGGLEFSACPFSGWYMGTEIGVRDYCDNSRYNILEEVAKKMNLDMRKTSSLWKDQALVEINIAVLYSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMLNYRLTPSFEYQPDPWNTHVWKGTNGTPTKRRAIGFKKLAEAVKFSAKLMGQAMAKRVKATILYATETGKSQAYAKTLCEIFKHAFDAKVMSMEEYDIVHLEHETLVLVVTSTFGNGDPPENGEKFGCALMEMRHPNSVQEERKSYKVRFNSVSSYSDSQKSSGDGPDLRDNFESAGPLANVRFSVFGLGSRAYPHFCAFGHAVDTLLEELGGERILKMREGDELCGQEEAFRTWAKKVFKAACDVFCVGDDVNIEKANNSLISNDRSWKRNKFRLTFVAEAPELTQGLSNVHKKRVSAARLLSRQNLQSPKSSRSTIFVRLHTNGSQELQYQPGDHLGVFPGNHEDLVNALIERLEDAPPVNQMVKVELLEERNTALGVISNWTDELRLPPCTIFQAFKYYLDITTPPTPLQLQQFASLATSEKEKQRLLVLSKGLQEYEEWKWGKNPTIVEVLEEFPSIQMPATLLLTQLSLLQPRYYSISSSPDMYPDEVHLTVAIVSYRTRDGEGPIHHGVCSSWLNRIQADELVPCFVRGAPSFHLPRNPQVPCILVGPGTGIAPFRSFWQQRQFDIQHKGMNPCPMVLVFGCRQSKIDHIYREETLQAKNKGVFRELYTAYSREPDKPKKYVQDILQEQLAESVYRALKEQGGHIYVCGDVTMAADVLKAIQRIMTQQGKLSAEDAGVFISRMRDDNRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDTDEVFSS

Target ORF information:

RefSeq Version	XM_017019347.1
Organism	Homo sapiens(human)
Definition	Homo sapiens nitric oxide synthase 1 (NOS1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017019347.1

ORF Insert Sequence:

ATGGAGGATC ACATGTTCGG TGTTCAGCAA ATCCAGCCCA ATGTCATTTC TGTTCGTCTC 
TTCAAGCGCA AAGTTGGGGG CCTGGGATTT CTGGTGAAGG AGCGGGTCAG TAAGCCGCCC 
GTGATCATCT CTGACCTGAT TCGTGGGGGC GCCGCAGAGC AGAGTGGCCT CATCCAGGCC 
GGAGACATCA TTCTTGCGGT CAACGGCCGG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC 
CTGGAGGTAC TCAGAGGCAT TGCCTCTGAG ACCCACGTGG TCCTCATTCT GAGGGGCCCT 
GAAGGTTTCA CCACGCACCT GGAGACCACC TTTACAGGTG ATGGGACCCC CAAGACCATC 
CGGGTGACAC AGCCCCTGGG TCCCCCCACC AAAGCCGTGG ATCTGTCCCA CCAGCCACCG 
GCCGGCAAAG AACAGCCCCT GGCAGTGGAT GGGGCCTCGG GTCCCGGGAA TGGGCCTCAG 
CATGCCTACG ATGATGGGCA GGAGGCTGGC TCACTCCCCC ATGCCAACGG CCTGGCCCCC 
AGGCCCCCAG GCCAGGACCC CGCGAAGAAA GCAACCAGAG TCAGCCTCCA AGGCAGAGGG 
GAGAACAATG AACTGCTCAA GGAGATAGAG CCTGTGCTGA GCCTTCTCAC CAGTGGGAGC 
AGAGGGGTCA AGGGAGGGGC ACCTGCCAAG GCAGAGATGA AAGATATGGG AATCCAGGTG 
GACAGAGATT TGGACGGCAA GTCACACAAA CCTCTGCCCC TCGGCGTGGA GAACGACCGA 
GTCTTCAATG ACCTATGGGG GAAGGGCAAT GTGCCTGTCG TCCTCAACAA CCCATATTCA 
GAGAAGGAGC AGCCCCCCAC CTCAGGAAAA CAGTCCCCCA CAAAGAATGG CAGCCCCTCC 
AAGTGTCCAC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG AGGTGGTTCT CACTGACACC 
CTCCACCTTA AGAGCACATT GGAAACGGGA TGCACTGAGT ACATCTGCAT GGGCTCCATC 
ATGCATCCTT CTCAGCATGC AAGGAGGCCT GAAGACGTCC GCACAAAAGG ACAGCTCTTC 
CCTCTCGCCA AAGAGTTTAT TGATCAATAC TATTCATCAA TTAAAAGATT TGGCTCCAAA 
GCCCACATGG AAAGGCTGGA AGAGGTGAAC AAAGAGATCG ACACCACTAG CACTTACCAG 
CTCAAGGACA CAGAGCTCAT CTATGGGGCC AAGCACGCCT GGCGGAATGC CTCGCGCTGT 
GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTATTCGATG CCCGTGACTG CACCACGGCC 
CACGGGATGT TCAACTACAT CTGTAACCAT GTCAAGTATG CCACCAACAA AGGGAACCTC 
AGGTCTGCCA TCACCATATT CCCCCAGAGG ACAGACGGCA AGCACGACTT CCGAGTCTGG 
AACTCCCAGC TCATCCGCTA CGCTGGCTAC AAGCAGCCTG ACGGCTCCAC CCTGGGGGAC 
CCAGCCAATG TGCAGTTCAC AGAGATATGC ATACAGCAGG GCTGGAAACC GCCTAGAGGC 
CGCTTCGATG TCCTGCCGCT CCTGCTTCAG GCCAACGGCA ATGACCCTGA GCTCTTCCAG 
ATTCCTCCAG AGCTGGTGTT GGAAGTTCCC ATCAGGCACC CCAAGTTTGA GTGGTTCAAG 
GACCTGGGGC TGAAGTGGTA CGGCCTCCCC GCCGTGTCCA ACATGCTCCT AGAGATTGGC 
GGCCTGGAGT TCAGCGCCTG TCCCTTCAGT GGCTGGTACA TGGGCACAGA GATTGGTGTC 
CGCGACTACT GTGACAACTC CCGCTACAAT ATCCTGGAGG AAGTGGCCAA GAAGATGAAC 
TTAGACATGA GGAAGACGTC CTCCCTGTGG AAGGACCAGG CGCTGGTGGA GATCAATATC 
GCGGTTCTCT ATAGCTTCCA GAGTGACAAA GTGACCATTG TTGACCATCA CTCCGCCACC 
GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGGGGCTG CCCTGCCGAC 
TGGGTGTGGA TCGTGCCCCC CATGTCCGGA AGCATCACCC CTGTGTTCCA CCAGGAGATG 
CTCAACTACC GGCTCACCCC CTCCTTCGAA TACCAGCCTG ATCCCTGGAA CACGCATGTC 
TGGAAAGGCA CCAACGGGAC CCCCACAAAG CGGCGAGCCA TTGGCTTCAA GAAGCTAGCA 
GAAGCTGTCA AGTTCTCGGC CAAGCTGATG GGGCAGGCTA TGGCCAAGAG GGTGAAAGCG 
ACCATCCTCT ATGCCACAGA GACAGGCAAA TCGCAAGCTT ATGCCAAGAC CTTGTGTGAG 
ATCTTCAAAC ACGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATTGTGCAC 
CTGGAACATG AAACTCTGGT CCTTGTGGTC ACCAGCACCT TTGGCAATGG AGATCCCCCT 
GAGAATGGGG AGAAATTCGG CTGTGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAG 
GAAGAAAGGA AGAGCTACAA GGTCCGATTC AACAGCGTCT CCTCCTACTC TGACTCCCAA 
AAATCATCAG GCGATGGGCC CGACCTCAGA GACAACTTTG AGAGTGCTGG ACCCCTGGCC 
AATGTGAGGT TCTCAGTTTT TGGCCTCGGC TCACGAGCAT ACCCTCACTT TTGCGCCTTC 
GGACACGCTG TGGACACCCT CCTGGAAGAA CTGGGAGGGG AGAGGATCCT GAAGATGAGG 
GAAGGGGATG AGCTCTGTGG GCAGGAAGAG GCTTTCAGGA CCTGGGCCAA GAAGGTCTTC 
AAGGCAGCCT GTGATGTCTT CTGTGTGGGA GATGATGTCA ACATTGAAAA GGCCAACAAT 
TCCCTCATCA GCAATGATCG CAGCTGGAAG AGAAACAAGT TCCGCCTCAC CTTTGTGGCC 
GAAGCTCCAG AACTCACACA AGGTCTATCC AATGTCCACA AAAAGCGAGT CTCAGCTGCC 
CGGCTCCTTA GCCGTCAAAA CCTCCAGAGC CCTAAATCCA GTCGGTCAAC TATCTTCGTG 
CGTCTCCACA CCAACGGGAG CCAGGAGCTG CAGTACCAGC CTGGGGACCA CCTGGGTGTC 
TTCCCTGGCA ACCACGAGGA CCTCGTGAAT GCCCTGATCG AGCGGCTGGA GGACGCGCCG 
CCTGTCAACC AGATGGTGAA AGTGGAACTG CTGGAGGAGC GGAACACGGC TTTAGGTGTC 
ATCAGTAACT GGACAGACGA GCTCCGCCTC CCGCCCTGCA CCATCTTCCA GGCCTTCAAG 
TACTACCTGG ACATCACCAC GCCACCAACG CCTCTGCAGC TGCAGCAGTT TGCCTCCCTA 
GCTACCAGCG AGAAGGAGAA GCAGCGTCTG CTGGTCCTCA GCAAGGGTTT GCAGGAGTAC 
GAGGAATGGA AATGGGGCAA GAACCCCACC ATCGTGGAGG TGCTGGAGGA GTTCCCATCT 
ATCCAGATGC CGGCCACCCT GCTCCTGACC CAGCTGTCCC TGCTGCAGCC CCGCTACTAT 
TCCATCAGCT CCTCCCCAGA CATGTACCCT GATGAAGTGC ACCTCACTGT GGCCATCGTT 
TCCTACCGCA CTCGAGATGG AGAAGGACCA ATTCACCACG GCGTATGCTC CTCCTGGCTC 
AACCGGATAC AGGCTGACGA ACTGGTCCCC TGTTTCGTGA GAGGAGCACC CAGCTTCCAC 
CTGCCCCGGA ACCCCCAAGT CCCCTGCATC CTCGTTGGAC CAGGCACCGG CATTGCCCCT 
TTCCGAAGCT TCTGGCAACA GCGGCAATTT GATATCCAAC ACAAAGGAAT GAACCCCTGC 
CCCATGGTCC TGGTCTTCGG GTGCCGGCAA TCCAAGATAG ATCATATCTA CAGGGAAGAG 
ACCCTGCAGG CCAAGAACAA GGGGGTCTTC AGAGAGCTGT ACACGGCTTA CTCCCGGGAG 
CCAGACAAAC CAAAGAAGTA CGTGCAGGAC ATCCTGCAGG AGCAGCTGGC GGAGTCTGTG 
TACCGAGCCC TGAAGGAGCA AGGGGGCCAC ATATACGTCT GTGGGGACGT CACCATGGCT 
GCTGATGTCC TCAAAGCCAT CCAGCGCATC ATGACCCAGC AGGGGAAGCT CTCGGCAGAG 
GACGCCGGCG TATTCATCAG CCGGATGAGG GATGACAACC GATACCATGA GGATATTTTT 
GGAGTCACCC TGCGAACGTA CGAAGTGACC AACCGCCTTA GATCTGAGTC CATTGCCTTC 
ATTGAAGAGA GCAAAAAAGA CACCGATGAG GTTTTCAGCT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOS1 ( XM_017019345.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016874834.1 Homo sapiens nitric oxide synthase 1 (NOS1), transcript variant X1, mRNA.

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CloneID	OHu21540
Clone ID Related Accession (Same CDS sequence)	XM_017019345.1 , XM_011538398.2 , XM_017019346.1 , NM_001204218.1
Accession Version	XM_017019345.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4407bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	nitric oxide synthase, brain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGATC ACATGTTCGG TGTTCAGCAA ATCCAGCCCA ATGTCATTTC TGTTCGTCTC 
TTCAAGCGCA AAGTTGGGGG CCTGGGATTT CTGGTGAAGG AGCGGGTCAG TAAGCCGCCC 
GTGATCATCT CTGACCTGAT TCGTGGGGGC GCCGCAGAGC AGAGTGGCCT CATCCAGGCC 
GGAGACATCA TTCTTGCGGT CAACGGCCGG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC 
CTGGAGGTAC TCAGAGGCAT TGCCTCTGAG ACCCACGTGG TCCTCATTCT GAGGGGCCCT 
GAAGGTTTCA CCACGCACCT GGAGACCACC TTTACAGGTG ATGGGACCCC CAAGACCATC 
CGGGTGACAC AGCCCCTGGG TCCCCCCACC AAAGCCGTGG ATCTGTCCCA CCAGCCACCG 
GCCGGCAAAG AACAGCCCCT GGCAGTGGAT GGGGCCTCGG GTCCCGGGAA TGGGCCTCAG 
CATGCCTACG ATGATGGGCA GGAGGCTGGC TCACTCCCCC ATGCCAACGG CCTGGCCCCC 
AGGCCCCCAG GCCAGGACCC CGCGAAGAAA GCAACCAGAG TCAGCCTCCA AGGCAGAGGG 
GAGAACAATG AACTGCTCAA GGAGATAGAG CCTGTGCTGA GCCTTCTCAC CAGTGGGAGC 
AGAGGGGTCA AGGGAGGGGC ACCTGCCAAG GCAGAGATGA AAGATATGGG AATCCAGGTG 
GACAGAGATT TGGACGGCAA GTCACACAAA CCTCTGCCCC TCGGCGTGGA GAACGACCGA 
GTCTTCAATG ACCTATGGGG GAAGGGCAAT GTGCCTGTCG TCCTCAACAA CCCATATTCA 
GAGAAGGAGC AGCCCCCCAC CTCAGGAAAA CAGTCCCCCA CAAAGAATGG CAGCCCCTCC 
AAGTGTCCAC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG AGGTGGTTCT CACTGACACC 
CTCCACCTTA AGAGCACATT GGAAACGGGA TGCACTGAGT ACATCTGCAT GGGCTCCATC 
ATGCATCCTT CTCAGCATGC AAGGAGGCCT GAAGACGTCC GCACAAAAGG ACAGCTCTTC 
CCTCTCGCCA AAGAGTTTAT TGATCAATAC TATTCATCAA TTAAAAGATT TGGCTCCAAA 
GCCCACATGG AAAGGCTGGA AGAGGTGAAC AAAGAGATCG ACACCACTAG CACTTACCAG 
CTCAAGGACA CAGAGCTCAT CTATGGGGCC AAGCACGCCT GGCGGAATGC CTCGCGCTGT 
GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTATTCGATG CCCGTGACTG CACCACGGCC 
CACGGGATGT TCAACTACAT CTGTAACCAT GTCAAGTATG CCACCAACAA AGGGAACCTC 
AGGTCTGCCA TCACCATATT CCCCCAGAGG ACAGACGGCA AGCACGACTT CCGAGTCTGG 
AACTCCCAGC TCATCCGCTA CGCTGGCTAC AAGCAGCCTG ACGGCTCCAC CCTGGGGGAC 
CCAGCCAATG TGCAGTTCAC AGAGATATGC ATACAGCAGG GCTGGAAACC GCCTAGAGGC 
CGCTTCGATG TCCTGCCGCT CCTGCTTCAG GCCAACGGCA ATGACCCTGA GCTCTTCCAG 
ATTCCTCCAG AGCTGGTGTT GGAAGTTCCC ATCAGGCACC CCAAGTTTGA GTGGTTCAAG 
GACCTGGGGC TGAAGTGGTA CGGCCTCCCC GCCGTGTCCA ACATGCTCCT AGAGATTGGC 
GGCCTGGAGT TCAGCGCCTG TCCCTTCAGT GGCTGGTACA TGGGCACAGA GATTGGTGTC 
CGCGACTACT GTGACAACTC CCGCTACAAT ATCCTGGAGG AAGTGGCCAA GAAGATGAAC 
TTAGACATGA GGAAGACGTC CTCCCTGTGG AAGGACCAGG CGCTGGTGGA GATCAATATC 
GCGGTTCTCT ATAGCTTCCA GAGTGACAAA GTGACCATTG TTGACCATCA CTCCGCCACC 
GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGGGGCTG CCCTGCCGAC 
TGGGTGTGGA TCGTGCCCCC CATGTCCGGA AGCATCACCC CTGTGTTCCA CCAGGAGATG 
CTCAACTACC GGCTCACCCC CTCCTTCGAA TACCAGCCTG ATCCCTGGAA CACGCATGTC 
TGGAAAGGCA CCAACGGGAC CCCCACAAAG CGGCGAGCCA TTGGCTTCAA GAAGCTAGCA 
GAAGCTGTCA AGTTCTCGGC CAAGCTGATG GGGCAGGCTA TGGCCAAGAG GGTGAAAGCG 
ACCATCCTCT ATGCCACAGA GACAGGCAAA TCGCAAGCTT ATGCCAAGAC CTTGTGTGAG 
ATCTTCAAAC ACGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATTGTGCAC 
CTGGAACATG AAACTCTGGT CCTTGTGGTC ACCAGCACCT TTGGCAATGG AGATCCCCCT 
GAGAATGGGG AGAAATTCGG CTGTGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAG 
GAAGAAAGGA AGTACCCGGA ACCCTTGCGT TTCTTTCCCC GTAAAGGGCC TCCCCTCCCC 
AATGGTGACA CAGAAGTCCA CGGTCTGGCT GCAGCCCGTG ACAGCCAGCA CAGGAGCTAC 
AAGGTCCGAT TCAACAGCGT CTCCTCCTAC TCTGACTCCC AAAAATCATC AGGCGATGGG 
CCCGACCTCA GAGACAACTT TGAGAGTGCT GGACCCCTGG CCAATGTGAG GTTCTCAGTT 
TTTGGCCTCG GCTCACGAGC ATACCCTCAC TTTTGCGCCT TCGGACACGC TGTGGACACC 
CTCCTGGAAG AACTGGGAGG GGAGAGGATC CTGAAGATGA GGGAAGGGGA TGAGCTCTGT 
GGGCAGGAAG AGGCTTTCAG GACCTGGGCC AAGAAGGTCT TCAAGGCAGC CTGTGATGTC 
TTCTGTGTGG GAGATGATGT CAACATTGAA AAGGCCAACA ATTCCCTCAT CAGCAATGAT 
CGCAGCTGGA AGAGAAACAA GTTCCGCCTC ACCTTTGTGG CCGAAGCTCC AGAACTCACA 
CAAGGTCTAT CCAATGTCCA CAAAAAGCGA GTCTCAGCTG CCCGGCTCCT TAGCCGTCAA 
AACCTCCAGA GCCCTAAATC CAGTCGGTCA ACTATCTTCG TGCGTCTCCA CACCAACGGG 
AGCCAGGAGC TGCAGTACCA GCCTGGGGAC CACCTGGGTG TCTTCCCTGG CAACCACGAG 
GACCTCGTGA ATGCCCTGAT CGAGCGGCTG GAGGACGCGC CGCCTGTCAA CCAGATGGTG 
AAAGTGGAAC TGCTGGAGGA GCGGAACACG GCTTTAGGTG TCATCAGTAA CTGGACAGAC 
GAGCTCCGCC TCCCGCCCTG CACCATCTTC CAGGCCTTCA AGTACTACCT GGACATCACC 
ACGCCACCAA CGCCTCTGCA GCTGCAGCAG TTTGCCTCCC TAGCTACCAG CGAGAAGGAG 
AAGCAGCGTC TGCTGGTCCT CAGCAAGGGT TTGCAGGAGT ACGAGGAATG GAAATGGGGC 
AAGAACCCCA CCATCGTGGA GGTGCTGGAG GAGTTCCCAT CTATCCAGAT GCCGGCCACC 
CTGCTCCTGA CCCAGCTGTC CCTGCTGCAG CCCCGCTACT ATTCCATCAG CTCCTCCCCA 
GACATGTACC CTGATGAAGT GCACCTCACT GTGGCCATCG TTTCCTACCG CACTCGAGAT 
GGAGAAGGAC CAATTCACCA CGGCGTATGC TCCTCCTGGC TCAACCGGAT ACAGGCTGAC 
GAACTGGTCC CCTGTTTCGT GAGAGGAGCA CCCAGCTTCC ACCTGCCCCG GAACCCCCAA 
GTCCCCTGCA TCCTCGTTGG ACCAGGCACC GGCATTGCCC CTTTCCGAAG CTTCTGGCAA 
CAGCGGCAAT TTGATATCCA ACACAAAGGA ATGAACCCCT GCCCCATGGT CCTGGTCTTC 
GGGTGCCGGC AATCCAAGAT AGATCATATC TACAGGGAAG AGACCCTGCA GGCCAAGAAC 
AAGGGGGTCT TCAGAGAGCT GTACACGGCT TACTCCCGGG AGCCAGACAA ACCAAAGAAG 
TACGTGCAGG ACATCCTGCA GGAGCAGCTG GCGGAGTCTG TGTACCGAGC CCTGAAGGAG 
CAAGGGGGCC ACATATACGT CTGTGGGGAC GTCACCATGG CTGCTGATGT CCTCAAAGCC 
ATCCAGCGCA TCATGACCCA GCAGGGGAAG CTCTCGGCAG AGGACGCCGG CGTATTCATC 
AGCCGGATGA GGGATGACAA CCGATACCAT GAGGATATTT TTGGAGTCAC CCTGCGAACG 
TACGAAGTGA CCAACCGCCT TAGATCTGAG TCCATTGCCT TCATTGAAGA GAGCAAAAAA 
GACACCGATG AGGTTTTCAG CTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016874834.1
CDS	719..5125
Translation	MEDHMFGVQQIQPNVISVRLFKRKVGGLGFLVKERVSKPPVIISDLIRGGAAEQSGLIQAGDIILAVNGRPLVDLSYDSALEVLRGIASETHVVLILRGPEGFTTHLETTFTGDGTPKTIRVTQPLGPPTKAVDLSHQPPAGKEQPLAVDGASGPGNGPQHAYDDGQEAGSLPHANGLAPRPPGQDPAKKATRVSLQGRGENNELLKEIEPVLSLLTSGSRGVKGGAPAKAEMKDMGIQVDRDLDGKSHKPLPLGVENDRVFNDLWGKGNVPVVLNNPYSEKEQPPTSGKQSPTKNGSPSKCPRFLKVKNWETEVVLTDTLHLKSTLETGCTEYICMGSIMHPSQHARRPEDVRTKGQLFPLAKEFIDQYYSSIKRFGSKAHMERLEEVNKEIDTTSTYQLKDTELIYGAKHAWRNASRCVGRIQWSKLQVFDARDCTTAHGMFNYICNHVKYATNKGNLRSAITIFPQRTDGKHDFRVWNSQLIRYAGYKQPDGSTLGDPANVQFTEICIQQGWKPPRGRFDVLPLLLQANGNDPELFQIPPELVLEVPIRHPKFEWFKDLGLKWYGLPAVSNMLLEIGGLEFSACPFSGWYMGTEIGVRDYCDNSRYNILEEVAKKMNLDMRKTSSLWKDQALVEINIAVLYSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMLNYRLTPSFEYQPDPWNTHVWKGTNGTPTKRRAIGFKKLAEAVKFSAKLMGQAMAKRVKATILYATETGKSQAYAKTLCEIFKHAFDAKVMSMEEYDIVHLEHETLVLVVTSTFGNGDPPENGEKFGCALMEMRHPNSVQEERKYPEPLRFFPRKGPPLPNGDTEVHGLAAARDSQHRSYKVRFNSVSSYSDSQKSSGDGPDLRDNFESAGPLANVRFSVFGLGSRAYPHFCAFGHAVDTLLEELGGERILKMREGDELCGQEEAFRTWAKKVFKAACDVFCVGDDVNIEKANNSLISNDRSWKRNKFRLTFVAEAPELTQGLSNVHKKRVSAARLLSRQNLQSPKSSRSTIFVRLHTNGSQELQYQPGDHLGVFPGNHEDLVNALIERLEDAPPVNQMVKVELLEERNTALGVISNWTDELRLPPCTIFQAFKYYLDITTPPTPLQLQQFASLATSEKEKQRLLVLSKGLQEYEEWKWGKNPTIVEVLEEFPSIQMPATLLLTQLSLLQPRYYSISSSPDMYPDEVHLTVAIVSYRTRDGEGPIHHGVCSSWLNRIQADELVPCFVRGAPSFHLPRNPQVPCILVGPGTGIAPFRSFWQQRQFDIQHKGMNPCPMVLVFGCRQSKIDHIYREETLQAKNKGVFRELYTAYSREPDKPKKYVQDILQEQLAESVYRALKEQGGHIYVCGDVTMAADVLKAIQRIMTQQGKLSAEDAGVFISRMRDDNRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDTDEVFSS

Target ORF information:

RefSeq Version	XM_017019345.1
Organism	Homo sapiens(human)
Definition	Homo sapiens nitric oxide synthase 1 (NOS1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017019345.1

ORF Insert Sequence:

ATGGAGGATC ACATGTTCGG TGTTCAGCAA ATCCAGCCCA ATGTCATTTC TGTTCGTCTC 
TTCAAGCGCA AAGTTGGGGG CCTGGGATTT CTGGTGAAGG AGCGGGTCAG TAAGCCGCCC 
GTGATCATCT CTGACCTGAT TCGTGGGGGC GCCGCAGAGC AGAGTGGCCT CATCCAGGCC 
GGAGACATCA TTCTTGCGGT CAACGGCCGG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC 
CTGGAGGTAC TCAGAGGCAT TGCCTCTGAG ACCCACGTGG TCCTCATTCT GAGGGGCCCT 
GAAGGTTTCA CCACGCACCT GGAGACCACC TTTACAGGTG ATGGGACCCC CAAGACCATC 
CGGGTGACAC AGCCCCTGGG TCCCCCCACC AAAGCCGTGG ATCTGTCCCA CCAGCCACCG 
GCCGGCAAAG AACAGCCCCT GGCAGTGGAT GGGGCCTCGG GTCCCGGGAA TGGGCCTCAG 
CATGCCTACG ATGATGGGCA GGAGGCTGGC TCACTCCCCC ATGCCAACGG CCTGGCCCCC 
AGGCCCCCAG GCCAGGACCC CGCGAAGAAA GCAACCAGAG TCAGCCTCCA AGGCAGAGGG 
GAGAACAATG AACTGCTCAA GGAGATAGAG CCTGTGCTGA GCCTTCTCAC CAGTGGGAGC 
AGAGGGGTCA AGGGAGGGGC ACCTGCCAAG GCAGAGATGA AAGATATGGG AATCCAGGTG 
GACAGAGATT TGGACGGCAA GTCACACAAA CCTCTGCCCC TCGGCGTGGA GAACGACCGA 
GTCTTCAATG ACCTATGGGG GAAGGGCAAT GTGCCTGTCG TCCTCAACAA CCCATATTCA 
GAGAAGGAGC AGCCCCCCAC CTCAGGAAAA CAGTCCCCCA CAAAGAATGG CAGCCCCTCC 
AAGTGTCCAC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG AGGTGGTTCT CACTGACACC 
CTCCACCTTA AGAGCACATT GGAAACGGGA TGCACTGAGT ACATCTGCAT GGGCTCCATC 
ATGCATCCTT CTCAGCATGC AAGGAGGCCT GAAGACGTCC GCACAAAAGG ACAGCTCTTC 
CCTCTCGCCA AAGAGTTTAT TGATCAATAC TATTCATCAA TTAAAAGATT TGGCTCCAAA 
GCCCACATGG AAAGGCTGGA AGAGGTGAAC AAAGAGATCG ACACCACTAG CACTTACCAG 
CTCAAGGACA CAGAGCTCAT CTATGGGGCC AAGCACGCCT GGCGGAATGC CTCGCGCTGT 
GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTATTCGATG CCCGTGACTG CACCACGGCC 
CACGGGATGT TCAACTACAT CTGTAACCAT GTCAAGTATG CCACCAACAA AGGGAACCTC 
AGGTCTGCCA TCACCATATT CCCCCAGAGG ACAGACGGCA AGCACGACTT CCGAGTCTGG 
AACTCCCAGC TCATCCGCTA CGCTGGCTAC AAGCAGCCTG ACGGCTCCAC CCTGGGGGAC 
CCAGCCAATG TGCAGTTCAC AGAGATATGC ATACAGCAGG GCTGGAAACC GCCTAGAGGC 
CGCTTCGATG TCCTGCCGCT CCTGCTTCAG GCCAACGGCA ATGACCCTGA GCTCTTCCAG 
ATTCCTCCAG AGCTGGTGTT GGAAGTTCCC ATCAGGCACC CCAAGTTTGA GTGGTTCAAG 
GACCTGGGGC TGAAGTGGTA CGGCCTCCCC GCCGTGTCCA ACATGCTCCT AGAGATTGGC 
GGCCTGGAGT TCAGCGCCTG TCCCTTCAGT GGCTGGTACA TGGGCACAGA GATTGGTGTC 
CGCGACTACT GTGACAACTC CCGCTACAAT ATCCTGGAGG AAGTGGCCAA GAAGATGAAC 
TTAGACATGA GGAAGACGTC CTCCCTGTGG AAGGACCAGG CGCTGGTGGA GATCAATATC 
GCGGTTCTCT ATAGCTTCCA GAGTGACAAA GTGACCATTG TTGACCATCA CTCCGCCACC 
GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGGGGCTG CCCTGCCGAC 
TGGGTGTGGA TCGTGCCCCC CATGTCCGGA AGCATCACCC CTGTGTTCCA CCAGGAGATG 
CTCAACTACC GGCTCACCCC CTCCTTCGAA TACCAGCCTG ATCCCTGGAA CACGCATGTC 
TGGAAAGGCA CCAACGGGAC CCCCACAAAG CGGCGAGCCA TTGGCTTCAA GAAGCTAGCA 
GAAGCTGTCA AGTTCTCGGC CAAGCTGATG GGGCAGGCTA TGGCCAAGAG GGTGAAAGCG 
ACCATCCTCT ATGCCACAGA GACAGGCAAA TCGCAAGCTT ATGCCAAGAC CTTGTGTGAG 
ATCTTCAAAC ACGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATTGTGCAC 
CTGGAACATG AAACTCTGGT CCTTGTGGTC ACCAGCACCT TTGGCAATGG AGATCCCCCT 
GAGAATGGGG AGAAATTCGG CTGTGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAG 
GAAGAAAGGA AGTACCCGGA ACCCTTGCGT TTCTTTCCCC GTAAAGGGCC TCCCCTCCCC 
AATGGTGACA CAGAAGTCCA CGGTCTGGCT GCAGCCCGTG ACAGCCAGCA CAGGAGCTAC 
AAGGTCCGAT TCAACAGCGT CTCCTCCTAC TCTGACTCCC AAAAATCATC AGGCGATGGG 
CCCGACCTCA GAGACAACTT TGAGAGTGCT GGACCCCTGG CCAATGTGAG GTTCTCAGTT 
TTTGGCCTCG GCTCACGAGC ATACCCTCAC TTTTGCGCCT TCGGACACGC TGTGGACACC 
CTCCTGGAAG AACTGGGAGG GGAGAGGATC CTGAAGATGA GGGAAGGGGA TGAGCTCTGT 
GGGCAGGAAG AGGCTTTCAG GACCTGGGCC AAGAAGGTCT TCAAGGCAGC CTGTGATGTC 
TTCTGTGTGG GAGATGATGT CAACATTGAA AAGGCCAACA ATTCCCTCAT CAGCAATGAT 
CGCAGCTGGA AGAGAAACAA GTTCCGCCTC ACCTTTGTGG CCGAAGCTCC AGAACTCACA 
CAAGGTCTAT CCAATGTCCA CAAAAAGCGA GTCTCAGCTG CCCGGCTCCT TAGCCGTCAA 
AACCTCCAGA GCCCTAAATC CAGTCGGTCA ACTATCTTCG TGCGTCTCCA CACCAACGGG 
AGCCAGGAGC TGCAGTACCA GCCTGGGGAC CACCTGGGTG TCTTCCCTGG CAACCACGAG 
GACCTCGTGA ATGCCCTGAT CGAGCGGCTG GAGGACGCGC CGCCTGTCAA CCAGATGGTG 
AAAGTGGAAC TGCTGGAGGA GCGGAACACG GCTTTAGGTG TCATCAGTAA CTGGACAGAC 
GAGCTCCGCC TCCCGCCCTG CACCATCTTC CAGGCCTTCA AGTACTACCT GGACATCACC 
ACGCCACCAA CGCCTCTGCA GCTGCAGCAG TTTGCCTCCC TAGCTACCAG CGAGAAGGAG 
AAGCAGCGTC TGCTGGTCCT CAGCAAGGGT TTGCAGGAGT ACGAGGAATG GAAATGGGGC 
AAGAACCCCA CCATCGTGGA GGTGCTGGAG GAGTTCCCAT CTATCCAGAT GCCGGCCACC 
CTGCTCCTGA CCCAGCTGTC CCTGCTGCAG CCCCGCTACT ATTCCATCAG CTCCTCCCCA 
GACATGTACC CTGATGAAGT GCACCTCACT GTGGCCATCG TTTCCTACCG CACTCGAGAT 
GGAGAAGGAC CAATTCACCA CGGCGTATGC TCCTCCTGGC TCAACCGGAT ACAGGCTGAC 
GAACTGGTCC CCTGTTTCGT GAGAGGAGCA CCCAGCTTCC ACCTGCCCCG GAACCCCCAA 
GTCCCCTGCA TCCTCGTTGG ACCAGGCACC GGCATTGCCC CTTTCCGAAG CTTCTGGCAA 
CAGCGGCAAT TTGATATCCA ACACAAAGGA ATGAACCCCT GCCCCATGGT CCTGGTCTTC 
GGGTGCCGGC AATCCAAGAT AGATCATATC TACAGGGAAG AGACCCTGCA GGCCAAGAAC 
AAGGGGGTCT TCAGAGAGCT GTACACGGCT TACTCCCGGG AGCCAGACAA ACCAAAGAAG 
TACGTGCAGG ACATCCTGCA GGAGCAGCTG GCGGAGTCTG TGTACCGAGC CCTGAAGGAG 
CAAGGGGGCC ACATATACGT CTGTGGGGAC GTCACCATGG CTGCTGATGT CCTCAAAGCC 
ATCCAGCGCA TCATGACCCA GCAGGGGAAG CTCTCGGCAG AGGACGCCGG CGTATTCATC 
AGCCGGATGA GGGATGACAA CCGATACCAT GAGGATATTT TTGGAGTCAC CCTGCGAACG 
TACGAAGTGA CCAACCGCCT TAGATCTGAG TCCATTGCCT TCATTGAAGA GAGCAAAAAA 
GACACCGATG AGGTTTTCAG CTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOS1 ( XM_011538398.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011536700.1 Homo sapiens nitric oxide synthase 1 (NOS1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu21540
Clone ID Related Accession (Same CDS sequence)	XM_017019345.1 , XM_011538398.2 , XM_017019346.1 , NM_001204218.1
Accession Version	XM_011538398.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4407bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	nitric oxide synthase, brain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011538398.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGATC ACATGTTCGG TGTTCAGCAA ATCCAGCCCA ATGTCATTTC TGTTCGTCTC 
TTCAAGCGCA AAGTTGGGGG CCTGGGATTT CTGGTGAAGG AGCGGGTCAG TAAGCCGCCC 
GTGATCATCT CTGACCTGAT TCGTGGGGGC GCCGCAGAGC AGAGTGGCCT CATCCAGGCC 
GGAGACATCA TTCTTGCGGT CAACGGCCGG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC 
CTGGAGGTAC TCAGAGGCAT TGCCTCTGAG ACCCACGTGG TCCTCATTCT GAGGGGCCCT 
GAAGGTTTCA CCACGCACCT GGAGACCACC TTTACAGGTG ATGGGACCCC CAAGACCATC 
CGGGTGACAC AGCCCCTGGG TCCCCCCACC AAAGCCGTGG ATCTGTCCCA CCAGCCACCG 
GCCGGCAAAG AACAGCCCCT GGCAGTGGAT GGGGCCTCGG GTCCCGGGAA TGGGCCTCAG 
CATGCCTACG ATGATGGGCA GGAGGCTGGC TCACTCCCCC ATGCCAACGG CCTGGCCCCC 
AGGCCCCCAG GCCAGGACCC CGCGAAGAAA GCAACCAGAG TCAGCCTCCA AGGCAGAGGG 
GAGAACAATG AACTGCTCAA GGAGATAGAG CCTGTGCTGA GCCTTCTCAC CAGTGGGAGC 
AGAGGGGTCA AGGGAGGGGC ACCTGCCAAG GCAGAGATGA AAGATATGGG AATCCAGGTG 
GACAGAGATT TGGACGGCAA GTCACACAAA CCTCTGCCCC TCGGCGTGGA GAACGACCGA 
GTCTTCAATG ACCTATGGGG GAAGGGCAAT GTGCCTGTCG TCCTCAACAA CCCATATTCA 
GAGAAGGAGC AGCCCCCCAC CTCAGGAAAA CAGTCCCCCA CAAAGAATGG CAGCCCCTCC 
AAGTGTCCAC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG AGGTGGTTCT CACTGACACC 
CTCCACCTTA AGAGCACATT GGAAACGGGA TGCACTGAGT ACATCTGCAT GGGCTCCATC 
ATGCATCCTT CTCAGCATGC AAGGAGGCCT GAAGACGTCC GCACAAAAGG ACAGCTCTTC 
CCTCTCGCCA AAGAGTTTAT TGATCAATAC TATTCATCAA TTAAAAGATT TGGCTCCAAA 
GCCCACATGG AAAGGCTGGA AGAGGTGAAC AAAGAGATCG ACACCACTAG CACTTACCAG 
CTCAAGGACA CAGAGCTCAT CTATGGGGCC AAGCACGCCT GGCGGAATGC CTCGCGCTGT 
GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTATTCGATG CCCGTGACTG CACCACGGCC 
CACGGGATGT TCAACTACAT CTGTAACCAT GTCAAGTATG CCACCAACAA AGGGAACCTC 
AGGTCTGCCA TCACCATATT CCCCCAGAGG ACAGACGGCA AGCACGACTT CCGAGTCTGG 
AACTCCCAGC TCATCCGCTA CGCTGGCTAC AAGCAGCCTG ACGGCTCCAC CCTGGGGGAC 
CCAGCCAATG TGCAGTTCAC AGAGATATGC ATACAGCAGG GCTGGAAACC GCCTAGAGGC 
CGCTTCGATG TCCTGCCGCT CCTGCTTCAG GCCAACGGCA ATGACCCTGA GCTCTTCCAG 
ATTCCTCCAG AGCTGGTGTT GGAAGTTCCC ATCAGGCACC CCAAGTTTGA GTGGTTCAAG 
GACCTGGGGC TGAAGTGGTA CGGCCTCCCC GCCGTGTCCA ACATGCTCCT AGAGATTGGC 
GGCCTGGAGT TCAGCGCCTG TCCCTTCAGT GGCTGGTACA TGGGCACAGA GATTGGTGTC 
CGCGACTACT GTGACAACTC CCGCTACAAT ATCCTGGAGG AAGTGGCCAA GAAGATGAAC 
TTAGACATGA GGAAGACGTC CTCCCTGTGG AAGGACCAGG CGCTGGTGGA GATCAATATC 
GCGGTTCTCT ATAGCTTCCA GAGTGACAAA GTGACCATTG TTGACCATCA CTCCGCCACC 
GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGGGGCTG CCCTGCCGAC 
TGGGTGTGGA TCGTGCCCCC CATGTCCGGA AGCATCACCC CTGTGTTCCA CCAGGAGATG 
CTCAACTACC GGCTCACCCC CTCCTTCGAA TACCAGCCTG ATCCCTGGAA CACGCATGTC 
TGGAAAGGCA CCAACGGGAC CCCCACAAAG CGGCGAGCCA TTGGCTTCAA GAAGCTAGCA 
GAAGCTGTCA AGTTCTCGGC CAAGCTGATG GGGCAGGCTA TGGCCAAGAG GGTGAAAGCG 
ACCATCCTCT ATGCCACAGA GACAGGCAAA TCGCAAGCTT ATGCCAAGAC CTTGTGTGAG 
ATCTTCAAAC ACGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATTGTGCAC 
CTGGAACATG AAACTCTGGT CCTTGTGGTC ACCAGCACCT TTGGCAATGG AGATCCCCCT 
GAGAATGGGG AGAAATTCGG CTGTGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAG 
GAAGAAAGGA AGTACCCGGA ACCCTTGCGT TTCTTTCCCC GTAAAGGGCC TCCCCTCCCC 
AATGGTGACA CAGAAGTCCA CGGTCTGGCT GCAGCCCGTG ACAGCCAGCA CAGGAGCTAC 
AAGGTCCGAT TCAACAGCGT CTCCTCCTAC TCTGACTCCC AAAAATCATC AGGCGATGGG 
CCCGACCTCA GAGACAACTT TGAGAGTGCT GGACCCCTGG CCAATGTGAG GTTCTCAGTT 
TTTGGCCTCG GCTCACGAGC ATACCCTCAC TTTTGCGCCT TCGGACACGC TGTGGACACC 
CTCCTGGAAG AACTGGGAGG GGAGAGGATC CTGAAGATGA GGGAAGGGGA TGAGCTCTGT 
GGGCAGGAAG AGGCTTTCAG GACCTGGGCC AAGAAGGTCT TCAAGGCAGC CTGTGATGTC 
TTCTGTGTGG GAGATGATGT CAACATTGAA AAGGCCAACA ATTCCCTCAT CAGCAATGAT 
CGCAGCTGGA AGAGAAACAA GTTCCGCCTC ACCTTTGTGG CCGAAGCTCC AGAACTCACA 
CAAGGTCTAT CCAATGTCCA CAAAAAGCGA GTCTCAGCTG CCCGGCTCCT TAGCCGTCAA 
AACCTCCAGA GCCCTAAATC CAGTCGGTCA ACTATCTTCG TGCGTCTCCA CACCAACGGG 
AGCCAGGAGC TGCAGTACCA GCCTGGGGAC CACCTGGGTG TCTTCCCTGG CAACCACGAG 
GACCTCGTGA ATGCCCTGAT CGAGCGGCTG GAGGACGCGC CGCCTGTCAA CCAGATGGTG 
AAAGTGGAAC TGCTGGAGGA GCGGAACACG GCTTTAGGTG TCATCAGTAA CTGGACAGAC 
GAGCTCCGCC TCCCGCCCTG CACCATCTTC CAGGCCTTCA AGTACTACCT GGACATCACC 
ACGCCACCAA CGCCTCTGCA GCTGCAGCAG TTTGCCTCCC TAGCTACCAG CGAGAAGGAG 
AAGCAGCGTC TGCTGGTCCT CAGCAAGGGT TTGCAGGAGT ACGAGGAATG GAAATGGGGC 
AAGAACCCCA CCATCGTGGA GGTGCTGGAG GAGTTCCCAT CTATCCAGAT GCCGGCCACC 
CTGCTCCTGA CCCAGCTGTC CCTGCTGCAG CCCCGCTACT ATTCCATCAG CTCCTCCCCA 
GACATGTACC CTGATGAAGT GCACCTCACT GTGGCCATCG TTTCCTACCG CACTCGAGAT 
GGAGAAGGAC CAATTCACCA CGGCGTATGC TCCTCCTGGC TCAACCGGAT ACAGGCTGAC 
GAACTGGTCC CCTGTTTCGT GAGAGGAGCA CCCAGCTTCC ACCTGCCCCG GAACCCCCAA 
GTCCCCTGCA TCCTCGTTGG ACCAGGCACC GGCATTGCCC CTTTCCGAAG CTTCTGGCAA 
CAGCGGCAAT TTGATATCCA ACACAAAGGA ATGAACCCCT GCCCCATGGT CCTGGTCTTC 
GGGTGCCGGC AATCCAAGAT AGATCATATC TACAGGGAAG AGACCCTGCA GGCCAAGAAC 
AAGGGGGTCT TCAGAGAGCT GTACACGGCT TACTCCCGGG AGCCAGACAA ACCAAAGAAG 
TACGTGCAGG ACATCCTGCA GGAGCAGCTG GCGGAGTCTG TGTACCGAGC CCTGAAGGAG 
CAAGGGGGCC ACATATACGT CTGTGGGGAC GTCACCATGG CTGCTGATGT CCTCAAAGCC 
ATCCAGCGCA TCATGACCCA GCAGGGGAAG CTCTCGGCAG AGGACGCCGG CGTATTCATC 
AGCCGGATGA GGGATGACAA CCGATACCAT GAGGATATTT TTGGAGTCAC CCTGCGAACG 
TACGAAGTGA CCAACCGCCT TAGATCTGAG TCCATTGCCT TCATTGAAGA GAGCAAAAAA 
GACACCGATG AGGTTTTCAG CTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011536700.1
CDS	583..4989
Translation	MEDHMFGVQQIQPNVISVRLFKRKVGGLGFLVKERVSKPPVIISDLIRGGAAEQSGLIQAGDIILAVNGRPLVDLSYDSALEVLRGIASETHVVLILRGPEGFTTHLETTFTGDGTPKTIRVTQPLGPPTKAVDLSHQPPAGKEQPLAVDGASGPGNGPQHAYDDGQEAGSLPHANGLAPRPPGQDPAKKATRVSLQGRGENNELLKEIEPVLSLLTSGSRGVKGGAPAKAEMKDMGIQVDRDLDGKSHKPLPLGVENDRVFNDLWGKGNVPVVLNNPYSEKEQPPTSGKQSPTKNGSPSKCPRFLKVKNWETEVVLTDTLHLKSTLETGCTEYICMGSIMHPSQHARRPEDVRTKGQLFPLAKEFIDQYYSSIKRFGSKAHMERLEEVNKEIDTTSTYQLKDTELIYGAKHAWRNASRCVGRIQWSKLQVFDARDCTTAHGMFNYICNHVKYATNKGNLRSAITIFPQRTDGKHDFRVWNSQLIRYAGYKQPDGSTLGDPANVQFTEICIQQGWKPPRGRFDVLPLLLQANGNDPELFQIPPELVLEVPIRHPKFEWFKDLGLKWYGLPAVSNMLLEIGGLEFSACPFSGWYMGTEIGVRDYCDNSRYNILEEVAKKMNLDMRKTSSLWKDQALVEINIAVLYSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMLNYRLTPSFEYQPDPWNTHVWKGTNGTPTKRRAIGFKKLAEAVKFSAKLMGQAMAKRVKATILYATETGKSQAYAKTLCEIFKHAFDAKVMSMEEYDIVHLEHETLVLVVTSTFGNGDPPENGEKFGCALMEMRHPNSVQEERKYPEPLRFFPRKGPPLPNGDTEVHGLAAARDSQHRSYKVRFNSVSSYSDSQKSSGDGPDLRDNFESAGPLANVRFSVFGLGSRAYPHFCAFGHAVDTLLEELGGERILKMREGDELCGQEEAFRTWAKKVFKAACDVFCVGDDVNIEKANNSLISNDRSWKRNKFRLTFVAEAPELTQGLSNVHKKRVSAARLLSRQNLQSPKSSRSTIFVRLHTNGSQELQYQPGDHLGVFPGNHEDLVNALIERLEDAPPVNQMVKVELLEERNTALGVISNWTDELRLPPCTIFQAFKYYLDITTPPTPLQLQQFASLATSEKEKQRLLVLSKGLQEYEEWKWGKNPTIVEVLEEFPSIQMPATLLLTQLSLLQPRYYSISSSPDMYPDEVHLTVAIVSYRTRDGEGPIHHGVCSSWLNRIQADELVPCFVRGAPSFHLPRNPQVPCILVGPGTGIAPFRSFWQQRQFDIQHKGMNPCPMVLVFGCRQSKIDHIYREETLQAKNKGVFRELYTAYSREPDKPKKYVQDILQEQLAESVYRALKEQGGHIYVCGDVTMAADVLKAIQRIMTQQGKLSAEDAGVFISRMRDDNRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDTDEVFSS

Target ORF information:

RefSeq Version	XM_011538398.2
Organism	Homo sapiens(human)
Definition	Homo sapiens nitric oxide synthase 1 (NOS1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011538398.2

ORF Insert Sequence:

ATGGAGGATC ACATGTTCGG TGTTCAGCAA ATCCAGCCCA ATGTCATTTC TGTTCGTCTC 
TTCAAGCGCA AAGTTGGGGG CCTGGGATTT CTGGTGAAGG AGCGGGTCAG TAAGCCGCCC 
GTGATCATCT CTGACCTGAT TCGTGGGGGC GCCGCAGAGC AGAGTGGCCT CATCCAGGCC 
GGAGACATCA TTCTTGCGGT CAACGGCCGG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC 
CTGGAGGTAC TCAGAGGCAT TGCCTCTGAG ACCCACGTGG TCCTCATTCT GAGGGGCCCT 
GAAGGTTTCA CCACGCACCT GGAGACCACC TTTACAGGTG ATGGGACCCC CAAGACCATC 
CGGGTGACAC AGCCCCTGGG TCCCCCCACC AAAGCCGTGG ATCTGTCCCA CCAGCCACCG 
GCCGGCAAAG AACAGCCCCT GGCAGTGGAT GGGGCCTCGG GTCCCGGGAA TGGGCCTCAG 
CATGCCTACG ATGATGGGCA GGAGGCTGGC TCACTCCCCC ATGCCAACGG CCTGGCCCCC 
AGGCCCCCAG GCCAGGACCC CGCGAAGAAA GCAACCAGAG TCAGCCTCCA AGGCAGAGGG 
GAGAACAATG AACTGCTCAA GGAGATAGAG CCTGTGCTGA GCCTTCTCAC CAGTGGGAGC 
AGAGGGGTCA AGGGAGGGGC ACCTGCCAAG GCAGAGATGA AAGATATGGG AATCCAGGTG 
GACAGAGATT TGGACGGCAA GTCACACAAA CCTCTGCCCC TCGGCGTGGA GAACGACCGA 
GTCTTCAATG ACCTATGGGG GAAGGGCAAT GTGCCTGTCG TCCTCAACAA CCCATATTCA 
GAGAAGGAGC AGCCCCCCAC CTCAGGAAAA CAGTCCCCCA CAAAGAATGG CAGCCCCTCC 
AAGTGTCCAC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG AGGTGGTTCT CACTGACACC 
CTCCACCTTA AGAGCACATT GGAAACGGGA TGCACTGAGT ACATCTGCAT GGGCTCCATC 
ATGCATCCTT CTCAGCATGC AAGGAGGCCT GAAGACGTCC GCACAAAAGG ACAGCTCTTC 
CCTCTCGCCA AAGAGTTTAT TGATCAATAC TATTCATCAA TTAAAAGATT TGGCTCCAAA 
GCCCACATGG AAAGGCTGGA AGAGGTGAAC AAAGAGATCG ACACCACTAG CACTTACCAG 
CTCAAGGACA CAGAGCTCAT CTATGGGGCC AAGCACGCCT GGCGGAATGC CTCGCGCTGT 
GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTATTCGATG CCCGTGACTG CACCACGGCC 
CACGGGATGT TCAACTACAT CTGTAACCAT GTCAAGTATG CCACCAACAA AGGGAACCTC 
AGGTCTGCCA TCACCATATT CCCCCAGAGG ACAGACGGCA AGCACGACTT CCGAGTCTGG 
AACTCCCAGC TCATCCGCTA CGCTGGCTAC AAGCAGCCTG ACGGCTCCAC CCTGGGGGAC 
CCAGCCAATG TGCAGTTCAC AGAGATATGC ATACAGCAGG GCTGGAAACC GCCTAGAGGC 
CGCTTCGATG TCCTGCCGCT CCTGCTTCAG GCCAACGGCA ATGACCCTGA GCTCTTCCAG 
ATTCCTCCAG AGCTGGTGTT GGAAGTTCCC ATCAGGCACC CCAAGTTTGA GTGGTTCAAG 
GACCTGGGGC TGAAGTGGTA CGGCCTCCCC GCCGTGTCCA ACATGCTCCT AGAGATTGGC 
GGCCTGGAGT TCAGCGCCTG TCCCTTCAGT GGCTGGTACA TGGGCACAGA GATTGGTGTC 
CGCGACTACT GTGACAACTC CCGCTACAAT ATCCTGGAGG AAGTGGCCAA GAAGATGAAC 
TTAGACATGA GGAAGACGTC CTCCCTGTGG AAGGACCAGG CGCTGGTGGA GATCAATATC 
GCGGTTCTCT ATAGCTTCCA GAGTGACAAA GTGACCATTG TTGACCATCA CTCCGCCACC 
GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGGGGCTG CCCTGCCGAC 
TGGGTGTGGA TCGTGCCCCC CATGTCCGGA AGCATCACCC CTGTGTTCCA CCAGGAGATG 
CTCAACTACC GGCTCACCCC CTCCTTCGAA TACCAGCCTG ATCCCTGGAA CACGCATGTC 
TGGAAAGGCA CCAACGGGAC CCCCACAAAG CGGCGAGCCA TTGGCTTCAA GAAGCTAGCA 
GAAGCTGTCA AGTTCTCGGC CAAGCTGATG GGGCAGGCTA TGGCCAAGAG GGTGAAAGCG 
ACCATCCTCT ATGCCACAGA GACAGGCAAA TCGCAAGCTT ATGCCAAGAC CTTGTGTGAG 
ATCTTCAAAC ACGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATTGTGCAC 
CTGGAACATG AAACTCTGGT CCTTGTGGTC ACCAGCACCT TTGGCAATGG AGATCCCCCT 
GAGAATGGGG AGAAATTCGG CTGTGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAG 
GAAGAAAGGA AGTACCCGGA ACCCTTGCGT TTCTTTCCCC GTAAAGGGCC TCCCCTCCCC 
AATGGTGACA CAGAAGTCCA CGGTCTGGCT GCAGCCCGTG ACAGCCAGCA CAGGAGCTAC 
AAGGTCCGAT TCAACAGCGT CTCCTCCTAC TCTGACTCCC AAAAATCATC AGGCGATGGG 
CCCGACCTCA GAGACAACTT TGAGAGTGCT GGACCCCTGG CCAATGTGAG GTTCTCAGTT 
TTTGGCCTCG GCTCACGAGC ATACCCTCAC TTTTGCGCCT TCGGACACGC TGTGGACACC 
CTCCTGGAAG AACTGGGAGG GGAGAGGATC CTGAAGATGA GGGAAGGGGA TGAGCTCTGT 
GGGCAGGAAG AGGCTTTCAG GACCTGGGCC AAGAAGGTCT TCAAGGCAGC CTGTGATGTC 
TTCTGTGTGG GAGATGATGT CAACATTGAA AAGGCCAACA ATTCCCTCAT CAGCAATGAT 
CGCAGCTGGA AGAGAAACAA GTTCCGCCTC ACCTTTGTGG CCGAAGCTCC AGAACTCACA 
CAAGGTCTAT CCAATGTCCA CAAAAAGCGA GTCTCAGCTG CCCGGCTCCT TAGCCGTCAA 
AACCTCCAGA GCCCTAAATC CAGTCGGTCA ACTATCTTCG TGCGTCTCCA CACCAACGGG 
AGCCAGGAGC TGCAGTACCA GCCTGGGGAC CACCTGGGTG TCTTCCCTGG CAACCACGAG 
GACCTCGTGA ATGCCCTGAT CGAGCGGCTG GAGGACGCGC CGCCTGTCAA CCAGATGGTG 
AAAGTGGAAC TGCTGGAGGA GCGGAACACG GCTTTAGGTG TCATCAGTAA CTGGACAGAC 
GAGCTCCGCC TCCCGCCCTG CACCATCTTC CAGGCCTTCA AGTACTACCT GGACATCACC 
ACGCCACCAA CGCCTCTGCA GCTGCAGCAG TTTGCCTCCC TAGCTACCAG CGAGAAGGAG 
AAGCAGCGTC TGCTGGTCCT CAGCAAGGGT TTGCAGGAGT ACGAGGAATG GAAATGGGGC 
AAGAACCCCA CCATCGTGGA GGTGCTGGAG GAGTTCCCAT CTATCCAGAT GCCGGCCACC 
CTGCTCCTGA CCCAGCTGTC CCTGCTGCAG CCCCGCTACT ATTCCATCAG CTCCTCCCCA 
GACATGTACC CTGATGAAGT GCACCTCACT GTGGCCATCG TTTCCTACCG CACTCGAGAT 
GGAGAAGGAC CAATTCACCA CGGCGTATGC TCCTCCTGGC TCAACCGGAT ACAGGCTGAC 
GAACTGGTCC CCTGTTTCGT GAGAGGAGCA CCCAGCTTCC ACCTGCCCCG GAACCCCCAA 
GTCCCCTGCA TCCTCGTTGG ACCAGGCACC GGCATTGCCC CTTTCCGAAG CTTCTGGCAA 
CAGCGGCAAT TTGATATCCA ACACAAAGGA ATGAACCCCT GCCCCATGGT CCTGGTCTTC 
GGGTGCCGGC AATCCAAGAT AGATCATATC TACAGGGAAG AGACCCTGCA GGCCAAGAAC 
AAGGGGGTCT TCAGAGAGCT GTACACGGCT TACTCCCGGG AGCCAGACAA ACCAAAGAAG 
TACGTGCAGG ACATCCTGCA GGAGCAGCTG GCGGAGTCTG TGTACCGAGC CCTGAAGGAG 
CAAGGGGGCC ACATATACGT CTGTGGGGAC GTCACCATGG CTGCTGATGT CCTCAAAGCC 
ATCCAGCGCA TCATGACCCA GCAGGGGAAG CTCTCGGCAG AGGACGCCGG CGTATTCATC 
AGCCGGATGA GGGATGACAA CCGATACCAT GAGGATATTT TTGGAGTCAC CCTGCGAACG 
TACGAAGTGA CCAACCGCCT TAGATCTGAG TCCATTGCCT TCATTGAAGA GAGCAAAAAA 
GACACCGATG AGGTTTTCAG CTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOS1 ( XM_017019346.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016874835.1 Homo sapiens nitric oxide synthase 1 (NOS1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu21540
Clone ID Related Accession (Same CDS sequence)	XM_017019345.1 , XM_011538398.2 , XM_017019346.1 , NM_001204218.1
Accession Version	XM_017019346.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4407bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	nitric oxide synthase, brain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGATC ACATGTTCGG TGTTCAGCAA ATCCAGCCCA ATGTCATTTC TGTTCGTCTC 
TTCAAGCGCA AAGTTGGGGG CCTGGGATTT CTGGTGAAGG AGCGGGTCAG TAAGCCGCCC 
GTGATCATCT CTGACCTGAT TCGTGGGGGC GCCGCAGAGC AGAGTGGCCT CATCCAGGCC 
GGAGACATCA TTCTTGCGGT CAACGGCCGG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC 
CTGGAGGTAC TCAGAGGCAT TGCCTCTGAG ACCCACGTGG TCCTCATTCT GAGGGGCCCT 
GAAGGTTTCA CCACGCACCT GGAGACCACC TTTACAGGTG ATGGGACCCC CAAGACCATC 
CGGGTGACAC AGCCCCTGGG TCCCCCCACC AAAGCCGTGG ATCTGTCCCA CCAGCCACCG 
GCCGGCAAAG AACAGCCCCT GGCAGTGGAT GGGGCCTCGG GTCCCGGGAA TGGGCCTCAG 
CATGCCTACG ATGATGGGCA GGAGGCTGGC TCACTCCCCC ATGCCAACGG CCTGGCCCCC 
AGGCCCCCAG GCCAGGACCC CGCGAAGAAA GCAACCAGAG TCAGCCTCCA AGGCAGAGGG 
GAGAACAATG AACTGCTCAA GGAGATAGAG CCTGTGCTGA GCCTTCTCAC CAGTGGGAGC 
AGAGGGGTCA AGGGAGGGGC ACCTGCCAAG GCAGAGATGA AAGATATGGG AATCCAGGTG 
GACAGAGATT TGGACGGCAA GTCACACAAA CCTCTGCCCC TCGGCGTGGA GAACGACCGA 
GTCTTCAATG ACCTATGGGG GAAGGGCAAT GTGCCTGTCG TCCTCAACAA CCCATATTCA 
GAGAAGGAGC AGCCCCCCAC CTCAGGAAAA CAGTCCCCCA CAAAGAATGG CAGCCCCTCC 
AAGTGTCCAC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG AGGTGGTTCT CACTGACACC 
CTCCACCTTA AGAGCACATT GGAAACGGGA TGCACTGAGT ACATCTGCAT GGGCTCCATC 
ATGCATCCTT CTCAGCATGC AAGGAGGCCT GAAGACGTCC GCACAAAAGG ACAGCTCTTC 
CCTCTCGCCA AAGAGTTTAT TGATCAATAC TATTCATCAA TTAAAAGATT TGGCTCCAAA 
GCCCACATGG AAAGGCTGGA AGAGGTGAAC AAAGAGATCG ACACCACTAG CACTTACCAG 
CTCAAGGACA CAGAGCTCAT CTATGGGGCC AAGCACGCCT GGCGGAATGC CTCGCGCTGT 
GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTATTCGATG CCCGTGACTG CACCACGGCC 
CACGGGATGT TCAACTACAT CTGTAACCAT GTCAAGTATG CCACCAACAA AGGGAACCTC 
AGGTCTGCCA TCACCATATT CCCCCAGAGG ACAGACGGCA AGCACGACTT CCGAGTCTGG 
AACTCCCAGC TCATCCGCTA CGCTGGCTAC AAGCAGCCTG ACGGCTCCAC CCTGGGGGAC 
CCAGCCAATG TGCAGTTCAC AGAGATATGC ATACAGCAGG GCTGGAAACC GCCTAGAGGC 
CGCTTCGATG TCCTGCCGCT CCTGCTTCAG GCCAACGGCA ATGACCCTGA GCTCTTCCAG 
ATTCCTCCAG AGCTGGTGTT GGAAGTTCCC ATCAGGCACC CCAAGTTTGA GTGGTTCAAG 
GACCTGGGGC TGAAGTGGTA CGGCCTCCCC GCCGTGTCCA ACATGCTCCT AGAGATTGGC 
GGCCTGGAGT TCAGCGCCTG TCCCTTCAGT GGCTGGTACA TGGGCACAGA GATTGGTGTC 
CGCGACTACT GTGACAACTC CCGCTACAAT ATCCTGGAGG AAGTGGCCAA GAAGATGAAC 
TTAGACATGA GGAAGACGTC CTCCCTGTGG AAGGACCAGG CGCTGGTGGA GATCAATATC 
GCGGTTCTCT ATAGCTTCCA GAGTGACAAA GTGACCATTG TTGACCATCA CTCCGCCACC 
GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGGGGCTG CCCTGCCGAC 
TGGGTGTGGA TCGTGCCCCC CATGTCCGGA AGCATCACCC CTGTGTTCCA CCAGGAGATG 
CTCAACTACC GGCTCACCCC CTCCTTCGAA TACCAGCCTG ATCCCTGGAA CACGCATGTC 
TGGAAAGGCA CCAACGGGAC CCCCACAAAG CGGCGAGCCA TTGGCTTCAA GAAGCTAGCA 
GAAGCTGTCA AGTTCTCGGC CAAGCTGATG GGGCAGGCTA TGGCCAAGAG GGTGAAAGCG 
ACCATCCTCT ATGCCACAGA GACAGGCAAA TCGCAAGCTT ATGCCAAGAC CTTGTGTGAG 
ATCTTCAAAC ACGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATTGTGCAC 
CTGGAACATG AAACTCTGGT CCTTGTGGTC ACCAGCACCT TTGGCAATGG AGATCCCCCT 
GAGAATGGGG AGAAATTCGG CTGTGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAG 
GAAGAAAGGA AGTACCCGGA ACCCTTGCGT TTCTTTCCCC GTAAAGGGCC TCCCCTCCCC 
AATGGTGACA CAGAAGTCCA CGGTCTGGCT GCAGCCCGTG ACAGCCAGCA CAGGAGCTAC 
AAGGTCCGAT TCAACAGCGT CTCCTCCTAC TCTGACTCCC AAAAATCATC AGGCGATGGG 
CCCGACCTCA GAGACAACTT TGAGAGTGCT GGACCCCTGG CCAATGTGAG GTTCTCAGTT 
TTTGGCCTCG GCTCACGAGC ATACCCTCAC TTTTGCGCCT TCGGACACGC TGTGGACACC 
CTCCTGGAAG AACTGGGAGG GGAGAGGATC CTGAAGATGA GGGAAGGGGA TGAGCTCTGT 
GGGCAGGAAG AGGCTTTCAG GACCTGGGCC AAGAAGGTCT TCAAGGCAGC CTGTGATGTC 
TTCTGTGTGG GAGATGATGT CAACATTGAA AAGGCCAACA ATTCCCTCAT CAGCAATGAT 
CGCAGCTGGA AGAGAAACAA GTTCCGCCTC ACCTTTGTGG CCGAAGCTCC AGAACTCACA 
CAAGGTCTAT CCAATGTCCA CAAAAAGCGA GTCTCAGCTG CCCGGCTCCT TAGCCGTCAA 
AACCTCCAGA GCCCTAAATC CAGTCGGTCA ACTATCTTCG TGCGTCTCCA CACCAACGGG 
AGCCAGGAGC TGCAGTACCA GCCTGGGGAC CACCTGGGTG TCTTCCCTGG CAACCACGAG 
GACCTCGTGA ATGCCCTGAT CGAGCGGCTG GAGGACGCGC CGCCTGTCAA CCAGATGGTG 
AAAGTGGAAC TGCTGGAGGA GCGGAACACG GCTTTAGGTG TCATCAGTAA CTGGACAGAC 
GAGCTCCGCC TCCCGCCCTG CACCATCTTC CAGGCCTTCA AGTACTACCT GGACATCACC 
ACGCCACCAA CGCCTCTGCA GCTGCAGCAG TTTGCCTCCC TAGCTACCAG CGAGAAGGAG 
AAGCAGCGTC TGCTGGTCCT CAGCAAGGGT TTGCAGGAGT ACGAGGAATG GAAATGGGGC 
AAGAACCCCA CCATCGTGGA GGTGCTGGAG GAGTTCCCAT CTATCCAGAT GCCGGCCACC 
CTGCTCCTGA CCCAGCTGTC CCTGCTGCAG CCCCGCTACT ATTCCATCAG CTCCTCCCCA 
GACATGTACC CTGATGAAGT GCACCTCACT GTGGCCATCG TTTCCTACCG CACTCGAGAT 
GGAGAAGGAC CAATTCACCA CGGCGTATGC TCCTCCTGGC TCAACCGGAT ACAGGCTGAC 
GAACTGGTCC CCTGTTTCGT GAGAGGAGCA CCCAGCTTCC ACCTGCCCCG GAACCCCCAA 
GTCCCCTGCA TCCTCGTTGG ACCAGGCACC GGCATTGCCC CTTTCCGAAG CTTCTGGCAA 
CAGCGGCAAT TTGATATCCA ACACAAAGGA ATGAACCCCT GCCCCATGGT CCTGGTCTTC 
GGGTGCCGGC AATCCAAGAT AGATCATATC TACAGGGAAG AGACCCTGCA GGCCAAGAAC 
AAGGGGGTCT TCAGAGAGCT GTACACGGCT TACTCCCGGG AGCCAGACAA ACCAAAGAAG 
TACGTGCAGG ACATCCTGCA GGAGCAGCTG GCGGAGTCTG TGTACCGAGC CCTGAAGGAG 
CAAGGGGGCC ACATATACGT CTGTGGGGAC GTCACCATGG CTGCTGATGT CCTCAAAGCC 
ATCCAGCGCA TCATGACCCA GCAGGGGAAG CTCTCGGCAG AGGACGCCGG CGTATTCATC 
AGCCGGATGA GGGATGACAA CCGATACCAT GAGGATATTT TTGGAGTCAC CCTGCGAACG 
TACGAAGTGA CCAACCGCCT TAGATCTGAG TCCATTGCCT TCATTGAAGA GAGCAAAAAA 
GACACCGATG AGGTTTTCAG CTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016874835.1
CDS	533..4939
Translation	MEDHMFGVQQIQPNVISVRLFKRKVGGLGFLVKERVSKPPVIISDLIRGGAAEQSGLIQAGDIILAVNGRPLVDLSYDSALEVLRGIASETHVVLILRGPEGFTTHLETTFTGDGTPKTIRVTQPLGPPTKAVDLSHQPPAGKEQPLAVDGASGPGNGPQHAYDDGQEAGSLPHANGLAPRPPGQDPAKKATRVSLQGRGENNELLKEIEPVLSLLTSGSRGVKGGAPAKAEMKDMGIQVDRDLDGKSHKPLPLGVENDRVFNDLWGKGNVPVVLNNPYSEKEQPPTSGKQSPTKNGSPSKCPRFLKVKNWETEVVLTDTLHLKSTLETGCTEYICMGSIMHPSQHARRPEDVRTKGQLFPLAKEFIDQYYSSIKRFGSKAHMERLEEVNKEIDTTSTYQLKDTELIYGAKHAWRNASRCVGRIQWSKLQVFDARDCTTAHGMFNYICNHVKYATNKGNLRSAITIFPQRTDGKHDFRVWNSQLIRYAGYKQPDGSTLGDPANVQFTEICIQQGWKPPRGRFDVLPLLLQANGNDPELFQIPPELVLEVPIRHPKFEWFKDLGLKWYGLPAVSNMLLEIGGLEFSACPFSGWYMGTEIGVRDYCDNSRYNILEEVAKKMNLDMRKTSSLWKDQALVEINIAVLYSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMLNYRLTPSFEYQPDPWNTHVWKGTNGTPTKRRAIGFKKLAEAVKFSAKLMGQAMAKRVKATILYATETGKSQAYAKTLCEIFKHAFDAKVMSMEEYDIVHLEHETLVLVVTSTFGNGDPPENGEKFGCALMEMRHPNSVQEERKYPEPLRFFPRKGPPLPNGDTEVHGLAAARDSQHRSYKVRFNSVSSYSDSQKSSGDGPDLRDNFESAGPLANVRFSVFGLGSRAYPHFCAFGHAVDTLLEELGGERILKMREGDELCGQEEAFRTWAKKVFKAACDVFCVGDDVNIEKANNSLISNDRSWKRNKFRLTFVAEAPELTQGLSNVHKKRVSAARLLSRQNLQSPKSSRSTIFVRLHTNGSQELQYQPGDHLGVFPGNHEDLVNALIERLEDAPPVNQMVKVELLEERNTALGVISNWTDELRLPPCTIFQAFKYYLDITTPPTPLQLQQFASLATSEKEKQRLLVLSKGLQEYEEWKWGKNPTIVEVLEEFPSIQMPATLLLTQLSLLQPRYYSISSSPDMYPDEVHLTVAIVSYRTRDGEGPIHHGVCSSWLNRIQADELVPCFVRGAPSFHLPRNPQVPCILVGPGTGIAPFRSFWQQRQFDIQHKGMNPCPMVLVFGCRQSKIDHIYREETLQAKNKGVFRELYTAYSREPDKPKKYVQDILQEQLAESVYRALKEQGGHIYVCGDVTMAADVLKAIQRIMTQQGKLSAEDAGVFISRMRDDNRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDTDEVFSS

Target ORF information:

RefSeq Version	XM_017019346.1
Organism	Homo sapiens(human)
Definition	Homo sapiens nitric oxide synthase 1 (NOS1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017019346.1

ORF Insert Sequence:

ATGGAGGATC ACATGTTCGG TGTTCAGCAA ATCCAGCCCA ATGTCATTTC TGTTCGTCTC 
TTCAAGCGCA AAGTTGGGGG CCTGGGATTT CTGGTGAAGG AGCGGGTCAG TAAGCCGCCC 
GTGATCATCT CTGACCTGAT TCGTGGGGGC GCCGCAGAGC AGAGTGGCCT CATCCAGGCC 
GGAGACATCA TTCTTGCGGT CAACGGCCGG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC 
CTGGAGGTAC TCAGAGGCAT TGCCTCTGAG ACCCACGTGG TCCTCATTCT GAGGGGCCCT 
GAAGGTTTCA CCACGCACCT GGAGACCACC TTTACAGGTG ATGGGACCCC CAAGACCATC 
CGGGTGACAC AGCCCCTGGG TCCCCCCACC AAAGCCGTGG ATCTGTCCCA CCAGCCACCG 
GCCGGCAAAG AACAGCCCCT GGCAGTGGAT GGGGCCTCGG GTCCCGGGAA TGGGCCTCAG 
CATGCCTACG ATGATGGGCA GGAGGCTGGC TCACTCCCCC ATGCCAACGG CCTGGCCCCC 
AGGCCCCCAG GCCAGGACCC CGCGAAGAAA GCAACCAGAG TCAGCCTCCA AGGCAGAGGG 
GAGAACAATG AACTGCTCAA GGAGATAGAG CCTGTGCTGA GCCTTCTCAC CAGTGGGAGC 
AGAGGGGTCA AGGGAGGGGC ACCTGCCAAG GCAGAGATGA AAGATATGGG AATCCAGGTG 
GACAGAGATT TGGACGGCAA GTCACACAAA CCTCTGCCCC TCGGCGTGGA GAACGACCGA 
GTCTTCAATG ACCTATGGGG GAAGGGCAAT GTGCCTGTCG TCCTCAACAA CCCATATTCA 
GAGAAGGAGC AGCCCCCCAC CTCAGGAAAA CAGTCCCCCA CAAAGAATGG CAGCCCCTCC 
AAGTGTCCAC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG AGGTGGTTCT CACTGACACC 
CTCCACCTTA AGAGCACATT GGAAACGGGA TGCACTGAGT ACATCTGCAT GGGCTCCATC 
ATGCATCCTT CTCAGCATGC AAGGAGGCCT GAAGACGTCC GCACAAAAGG ACAGCTCTTC 
CCTCTCGCCA AAGAGTTTAT TGATCAATAC TATTCATCAA TTAAAAGATT TGGCTCCAAA 
GCCCACATGG AAAGGCTGGA AGAGGTGAAC AAAGAGATCG ACACCACTAG CACTTACCAG 
CTCAAGGACA CAGAGCTCAT CTATGGGGCC AAGCACGCCT GGCGGAATGC CTCGCGCTGT 
GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTATTCGATG CCCGTGACTG CACCACGGCC 
CACGGGATGT TCAACTACAT CTGTAACCAT GTCAAGTATG CCACCAACAA AGGGAACCTC 
AGGTCTGCCA TCACCATATT CCCCCAGAGG ACAGACGGCA AGCACGACTT CCGAGTCTGG 
AACTCCCAGC TCATCCGCTA CGCTGGCTAC AAGCAGCCTG ACGGCTCCAC CCTGGGGGAC 
CCAGCCAATG TGCAGTTCAC AGAGATATGC ATACAGCAGG GCTGGAAACC GCCTAGAGGC 
CGCTTCGATG TCCTGCCGCT CCTGCTTCAG GCCAACGGCA ATGACCCTGA GCTCTTCCAG 
ATTCCTCCAG AGCTGGTGTT GGAAGTTCCC ATCAGGCACC CCAAGTTTGA GTGGTTCAAG 
GACCTGGGGC TGAAGTGGTA CGGCCTCCCC GCCGTGTCCA ACATGCTCCT AGAGATTGGC 
GGCCTGGAGT TCAGCGCCTG TCCCTTCAGT GGCTGGTACA TGGGCACAGA GATTGGTGTC 
CGCGACTACT GTGACAACTC CCGCTACAAT ATCCTGGAGG AAGTGGCCAA GAAGATGAAC 
TTAGACATGA GGAAGACGTC CTCCCTGTGG AAGGACCAGG CGCTGGTGGA GATCAATATC 
GCGGTTCTCT ATAGCTTCCA GAGTGACAAA GTGACCATTG TTGACCATCA CTCCGCCACC 
GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGGGGCTG CCCTGCCGAC 
TGGGTGTGGA TCGTGCCCCC CATGTCCGGA AGCATCACCC CTGTGTTCCA CCAGGAGATG 
CTCAACTACC GGCTCACCCC CTCCTTCGAA TACCAGCCTG ATCCCTGGAA CACGCATGTC 
TGGAAAGGCA CCAACGGGAC CCCCACAAAG CGGCGAGCCA TTGGCTTCAA GAAGCTAGCA 
GAAGCTGTCA AGTTCTCGGC CAAGCTGATG GGGCAGGCTA TGGCCAAGAG GGTGAAAGCG 
ACCATCCTCT ATGCCACAGA GACAGGCAAA TCGCAAGCTT ATGCCAAGAC CTTGTGTGAG 
ATCTTCAAAC ACGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATTGTGCAC 
CTGGAACATG AAACTCTGGT CCTTGTGGTC ACCAGCACCT TTGGCAATGG AGATCCCCCT 
GAGAATGGGG AGAAATTCGG CTGTGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAG 
GAAGAAAGGA AGTACCCGGA ACCCTTGCGT TTCTTTCCCC GTAAAGGGCC TCCCCTCCCC 
AATGGTGACA CAGAAGTCCA CGGTCTGGCT GCAGCCCGTG ACAGCCAGCA CAGGAGCTAC 
AAGGTCCGAT TCAACAGCGT CTCCTCCTAC TCTGACTCCC AAAAATCATC AGGCGATGGG 
CCCGACCTCA GAGACAACTT TGAGAGTGCT GGACCCCTGG CCAATGTGAG GTTCTCAGTT 
TTTGGCCTCG GCTCACGAGC ATACCCTCAC TTTTGCGCCT TCGGACACGC TGTGGACACC 
CTCCTGGAAG AACTGGGAGG GGAGAGGATC CTGAAGATGA GGGAAGGGGA TGAGCTCTGT 
GGGCAGGAAG AGGCTTTCAG GACCTGGGCC AAGAAGGTCT TCAAGGCAGC CTGTGATGTC 
TTCTGTGTGG GAGATGATGT CAACATTGAA AAGGCCAACA ATTCCCTCAT CAGCAATGAT 
CGCAGCTGGA AGAGAAACAA GTTCCGCCTC ACCTTTGTGG CCGAAGCTCC AGAACTCACA 
CAAGGTCTAT CCAATGTCCA CAAAAAGCGA GTCTCAGCTG CCCGGCTCCT TAGCCGTCAA 
AACCTCCAGA GCCCTAAATC CAGTCGGTCA ACTATCTTCG TGCGTCTCCA CACCAACGGG 
AGCCAGGAGC TGCAGTACCA GCCTGGGGAC CACCTGGGTG TCTTCCCTGG CAACCACGAG 
GACCTCGTGA ATGCCCTGAT CGAGCGGCTG GAGGACGCGC CGCCTGTCAA CCAGATGGTG 
AAAGTGGAAC TGCTGGAGGA GCGGAACACG GCTTTAGGTG TCATCAGTAA CTGGACAGAC 
GAGCTCCGCC TCCCGCCCTG CACCATCTTC CAGGCCTTCA AGTACTACCT GGACATCACC 
ACGCCACCAA CGCCTCTGCA GCTGCAGCAG TTTGCCTCCC TAGCTACCAG CGAGAAGGAG 
AAGCAGCGTC TGCTGGTCCT CAGCAAGGGT TTGCAGGAGT ACGAGGAATG GAAATGGGGC 
AAGAACCCCA CCATCGTGGA GGTGCTGGAG GAGTTCCCAT CTATCCAGAT GCCGGCCACC 
CTGCTCCTGA CCCAGCTGTC CCTGCTGCAG CCCCGCTACT ATTCCATCAG CTCCTCCCCA 
GACATGTACC CTGATGAAGT GCACCTCACT GTGGCCATCG TTTCCTACCG CACTCGAGAT 
GGAGAAGGAC CAATTCACCA CGGCGTATGC TCCTCCTGGC TCAACCGGAT ACAGGCTGAC 
GAACTGGTCC CCTGTTTCGT GAGAGGAGCA CCCAGCTTCC ACCTGCCCCG GAACCCCCAA 
GTCCCCTGCA TCCTCGTTGG ACCAGGCACC GGCATTGCCC CTTTCCGAAG CTTCTGGCAA 
CAGCGGCAAT TTGATATCCA ACACAAAGGA ATGAACCCCT GCCCCATGGT CCTGGTCTTC 
GGGTGCCGGC AATCCAAGAT AGATCATATC TACAGGGAAG AGACCCTGCA GGCCAAGAAC 
AAGGGGGTCT TCAGAGAGCT GTACACGGCT TACTCCCGGG AGCCAGACAA ACCAAAGAAG 
TACGTGCAGG ACATCCTGCA GGAGCAGCTG GCGGAGTCTG TGTACCGAGC CCTGAAGGAG 
CAAGGGGGCC ACATATACGT CTGTGGGGAC GTCACCATGG CTGCTGATGT CCTCAAAGCC 
ATCCAGCGCA TCATGACCCA GCAGGGGAAG CTCTCGGCAG AGGACGCCGG CGTATTCATC 
AGCCGGATGA GGGATGACAA CCGATACCAT GAGGATATTT TTGGAGTCAC CCTGCGAACG 
TACGAAGTGA CCAACCGCCT TAGATCTGAG TCCATTGCCT TCATTGAAGA GAGCAAAAAA 
GACACCGATG AGGTTTTCAG CTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	NOS1
Entrez Gene ID	4842
Full Name	nitric oxide synthase 1
Synonyms	IHPS1,N-NOS,NC-NOS,NOS,bNOS,nNOS
General protein information	Preferred Names nitric oxide synthase 1 Names nitric oxide synthase, brain NOS type I constitutive NOS neuronal NOS nitric oxide synthase 1 (neuronal) peptidyl-cysteine S-nitrosylase NOS1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q24.22
Summary	The protein encoded by this gene belongs to the family of nitric oxide synthases, which synthesize nitric oxide from L-arginine. Nitric oxide is a reactive free radical, which acts as a biologic mediator in several processes, including neurotransmission, and antimicrobial and antitumoral activities. In the brain and peripheral nervous system, nitric oxide displays many properties of a neurotransmitter, and has been implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. This protein is ubiquitously expressed, with high level of expression in skeletal muscle. Multiple transcript variants that differ in the 5' UTR have been described for this gene but the full-length nature of these transcripts is not known. Additionally, alternatively spliced transcript variants encoding different isoforms (some testis-specific) have been found for this gene.[provided by RefSeq, Feb 2011].
Disorder MIM:	163731

Pan troglodytes (chimpanzee)	NOS1	XP_003314004.2
Rattus norvegicus (Norway rat)	Nos1	NP_434686.1
Gallus gallus (chicken)	NOS1	XP_004934537.1
Xenopus tropicalis (tropical clawed frog)	nos1	XP_004910558.1
	NOS1	XP_001083352.2
Mus musculus (house mouse)	Nos1	NP_032738.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008255	XP_317213.1
Homo sapiens (human)	NOS1	NP_000611.1
Bos taurus (cattle)	NOS1	XP_002694631.3
Canis lupus familiaris (dog)	NOS1	NP_001182074.1
Danio rerio (zebrafish)	nos1	NP_571735.1
Drosophila melanogaster (fruit fly)	Nos	NP_523541.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

NOS1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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