Nos cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nos
Entrez Gene ID	34495
Full Name	Nitric oxide synthase
Synonyms	CG6713,DNOS,DNOS1,Dmel\CG6713,NOS,dNOS,dNos,drNOSoxy,nNOS,nos
General protein information	Preferred Names Nitric oxide synthase Names CG6713 gene product from transcript CG6713-RA CG6713-PA CG6713-PF CG6713-PK NO synthase Nos-PA Nos-PF Nos-PK nitric oixide synthase nitric oxide synthase nitric oxidesynthase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 32B1-32B1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001032072.2	NP_001027243.2	nitric oxide synthase, isoform K
NM_001032069.2	NP_001027240.1	nitric oxide synthase, isoform F
NM_078817.4	NP_523541.2	nitric oxide synthase, isoform A

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NOS1	XP_003314004.2
Rattus norvegicus (Norway rat)	Nos1	NP_434686.1
Gallus gallus (chicken)	NOS1	XP_004934537.1
Xenopus tropicalis (tropical clawed frog)	nos1	XP_004910558.1
	NOS1	XP_001083352.2
Mus musculus (house mouse)	Nos1	NP_032738.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008255	XP_317213.1
Homo sapiens (human)	NOS1	NP_000611.1
Bos taurus (cattle)	NOS1	XP_002694631.3
Canis lupus familiaris (dog)	NOS1	NP_001182074.1
Danio rerio (zebrafish)	nos1	NP_571735.1
Drosophila melanogaster (fruit fly)	Nos	NP_523541.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Here we demonstrate that the Drosophila relaxin receptor homolog Lgr3, a leucine-rich repeat-containing G-protein-coupled receptor, is required for Dilp8-dependent growth coordination and developmental delay during the regeneration checkpoint; we demonstrate that Lgr3 activity in both the CNS and PG is necessary for NOS activation in the PG following damage
Title: Growth Coordination During Drosophila melanogaster Imaginal Disc Regeneration Is Mediated by Signaling Through the Relaxin Receptor Lgr3 in the Prothoracic Gland.

Nitric Oxide Synthase Regulates Growth Coordination During Drosophila melanogaster Imaginal Disc Regeneration
Title: Nitric Oxide Synthase Regulates Growth Coordination During Drosophila melanogaster Imaginal Disc Regeneration.

Hb9 governs neuronal specification and differentiation and activates expression of Nos and fd59a in the Drosophila CNS
Title: Genome-wide identification of Drosophila Hb9 targets reveals a pivotal role in directing the transcriptome within eight neuronal lineages, including activation of nitric oxide synthase and Fd59a/Fox-D.

Nitric oxide synthase is not essential for Drosophila development.
Title: Nitric oxide synthase is not essential for Drosophila development.

The suppressed enzyme expression was shown to affect the cell polarity and movement as well as the structural pattern of organs during Drosophila development.
Title: [Nitric oxide synthase mediates regulation of cell polarity and movement during Drosophila melanogaster morphogenesis].

The following Nos gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nos cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa21623	NM_001032072.2 Latest version!	Drosophila melanogaster nitric oxide synthase, transcript variant K (Nos), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa14413	NM_001032069.2 Latest version!	Drosophila melanogaster nitric oxide synthase, transcript variant F (Nos), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OFa14414	NM_078817.4 Latest version!	Drosophila melanogaster nitric oxide synthase, transcript variant A (Nos), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nos ( NM_001032072.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001027243.2 Drosophila melanogaster nitric oxide synthase, transcript variant K (Nos), mRNA.

Price & Availability↑

CloneID	OFa21623
Clone ID Related Accession (Same CDS sequence)	NM_001032072.2
Accession Version	NM_001032072.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3297bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nitric oxide synthase, isoform K
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001032072.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACATTG GCAATGCGGC GGTGGAGGCC AGGAAATCCG ATCTGATCCT CGAACACGCC 
AAGGACTTCC TCGAGCAGTA CTTTACATCG ATAAAGCGTA CATCATCTAC CGCCCACGAG 
ACGCGATGGA AACAGGTGCG CCAGAGCATT GAGACCACTG GACATTATCA GCTAACCGAA 
ACGGAACTAA TTTATGGTGC CAAATTGGCC TGGCGCAATT CTTCACGTTG CATTGGCCGA 
ATACAATGGT CGAAGTTGCA GGTCTTTGAC TGTCGGTATG TGACAACAAC AAGTGGCATG 
TTTGAAGCCA TTTGCAATCA CATTAAATAT GCAACAAATA AGGGCAACCT GAGATCGGCC 
ATCACGATAT TTCCACAACG CACAGATGCC AAGCATGATT ATCGCATTTG GAATAACCAA 
TTAATATCTT ATGCCGGCTA CAAGCAGGCG GATGGGAAAA TCATTGGCGA TCCCATGAAT 
GTGGAGTTTA CAGAGGTCTG CACCAAGCTG GGCTGGAAGA GCAAGGGCAG CGAGTGGGAC 
ATACTGCCAT TGGTGGTCTC GGCCAATGGT CACGATCCGG ACTACTTTGA TTACCCGCCC 
GAATTGATAC TGGAAGTTCC GCTGACCCAT CCAAAATTCG AATGGTTCTC GGATCTGGGA 
CTGCGATGGT ACGCCCTGCC CGCCGTATCC AGTATGCTGT TCGATGTGGG CGGCATTCAG 
TTTACGGCCA CCACATTCAG TGGTTGGTAC ATGTCGACAG AGATTGGCAG CCGGAATTTA 
TGCGACACAA ATCGCCGCAA TATGCTGGAG ACGGTGGCGC TGAAGATGCA ACTGGACACC 
CGTACGCCCA CATCCTTGTG GAAGGACAAG GCTGTGGTGG AGATGAACAT TGCCGTGCTC 
CACTCCTACC AGAGTCGCAA CGTGACCATT GTGGATCACC ACACGGCCAG CGAGAGCTTT 
ATGAAGCATT TCGAGAACGA GTCCAAGCTC AGGAATGGGT GTCCCGCTGA TTGGATTTGG 
ATCGTGCCGC CGCTGTCGGG CTCCATAACG CCGGTATTCC ATCAGGAGAT GGCTCTGTAC 
TACCTGAAGC CCTCGTTCGA GTACCAGGAT CCCGCCTGGC GAACCCACGT GTGGAAGAAG 
GGGCGTGGCG AGAGCAAGGG CAAGAAGCCA AGACGTAAAT TCAATTTTAA ACAAATCGCT 
AGGGCTGTGA AATTTACATC GAAACTATTT GGACGCGCCT TATCGAAACG CATAAAGGCA 
ACAGTTCTAT ATGCCACCGA AACTGGCAAA TCGGAGCAAT ATGCGAAGCA ACTTTGTGAA 
CTCCTAGGGC ACGCATTCAA TGCACAGATA TATTGCATGT CCGACTACGA TATATCCTCC 
ATTGAGCACG AGGCATTGTT AATTGTTGTG GCCTCCACCT TTGGCAACGG TGATCCGCCC 
GAAAACGGCG AGCTTTTCTC CCAGGAATTG TATGCGATGC GTGTCCAGGA GTCTTCCGAG 
CATGGATTGC AGGACTCCAG CATTGGCTCG TCAAAGTCCT TCATGAAGGC CAGCTCGCGG 
CAGGAGTTCA TGAAGCTGCC ACTGCAACAG GTGAAGAGAA TCGACCGCTG GGACTCGCTG 
CGGGGCTCCA CCTCGGACAC CTTCACCGAG GAGACCTTTG GCCCCCTCTC CAATGTCCGG 
TTCGCCGTTT TTGCCCTCGG CTCCTCGGCC TATCCAAATT TCTGCGCCTT CGGTCAGTAT 
GTGGACAACA TTCTGGGCGA GCTGGGCGGC GAACGCCTGC TGAGGGTGGC CTACGGCGAC 
GAGATGTGCG GACAGGAGCA GTCGTTCCGG AAGTGGGCGC CCGAGGTATT CAAGTTGGCC 
TGCGAGACCT TCTGCCTGGA TCCAGAGGAG AGCCTTTCGG ATGCCTCGCT AGCCCTGCAG 
AACGATTCGC TGACTGTGAA TACGGTGCGC CTGGTGCCGT CGGCGAATAA GGGATCCCTG 
GACAGCAGTT TATCCAAGTA CCACAACAAG AAGGTGCACT GCTGCAAGGC GAAGGCGAAG 
CCCCACAATT TGACCCGTTT GAGTGAGGGA GCCAAGACAA CGATGCTGCT GGAGATCTGT 
GCACCTGGCT TGGAGTACGA GCCGGGTGAT CATGTGGGCA TCTTTCCGGC GAATCGAACG 
GAACTGGTCG ACGGACTGCT AAATCGACTG GTGGGTGTGG ATAATCCCGA CGAGGTGCTG 
CAGTTGCAAT TGCTAAAGGA AAAGCAGACA TCGAATGGTA TATTCAAGTG CTGGGAGCCG 
CACGACAAAA TACCGCCGGA TACTCTAAGG AATCTTCTGG CCCGATTCTT TGATCTGACC 
ACTCCGCCAT CGCGACAGTT GCTCACCCTG CTGGCAGGAT TCTGTGAGGA TACCGCGGAC 
AAGGAGCGGC TGGAGTTGCT GGTCAACGAT TCGTCGGCCT ACGAGGACTG GCGGCACTGG 
CGGCTGCCCC ATCTGCTGGA CGTTCTCGAG GAGTTCCCCT CGTGCCGACC ACCGGCTCCC 
CTTCTGCTTG CCCAACTAAC GCCGCTGCAG CCTCGCTTCT ATTCCATTTC TTCGTCGCCG 
CGCCGCGTTA GTGACGAAAT CCACCTCACG GTGGCCATCG TGAAGTACCG CTGTGAAGAT 
GGCCAGGGAG ACGAGCGGTA TGGCGTGTGC TCTAACTATC TATCCGGCTT GCGGGCAGAC 
GACGAGCTGT TCATGTTCGT GAGGAGCGCC TTGGGCTTCC ATTTGCCCAG CGATCGGAGT 
CGTCCCATTA TTCTGATTGG TCCTGGCACA GGAATAGCTC CATTCCGCTC CTTTTGGCAG 
GAGTTCCAGG TGCTAAGCGA CCTTGATCCC ACGGCCAAAT TGCCCAAGAT GTGGCTCTTC 
TTTGGCTGCC GTAATCGGGA TGTCGATTTG TACGCCGAGG AGAAGGCAGA GCTACAGAAG 
GATCAAATCC TAGACCGAGT TTTTCTCGCT CTGTCCAGGG AGCAGGCCAT TCCGAAGACA 
TATGTGCAGG ACCTGATTGA GCAGGAATTC GATTCGTTGT ACCAGTTGAT TGTCCAGGAG 
CGGGGCCACA TCTACGTCTG CGGCGATGTC ACAATGGCCG AGCATGTGTA CCAGACCATC 
AGGAAGTGCA TTGCCGGCAA AGAGCAGAAA AGCGAGGCGG AAGTTGAGAC ATTTTTGCTA 
ACACTGCGGG ACGAAAGTCG CTACCACGAG GACATCTTTG GCATCACGCT GCGAACGGCT 
GAGATACACA CAAAGTCAAG GGCCACGGCC AGGATACGAA TGGCCTCCCA GCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001027243.2
CDS	459..3755
Translation	MNIGNAAVEARKSDLILEHAKDFLEQYFTSIKRTSSTAHETRWKQVRQSIETTGHYQLTETELIYGAKLAWRNSSRCIGRIQWSKLQVFDCRYVTTTSGMFEAICNHIKYATNKGNLRSAITIFPQRTDAKHDYRIWNNQLISYAGYKQADGKIIGDPMNVEFTEVCTKLGWKSKGSEWDILPLVVSANGHDPDYFDYPPELILEVPLTHPKFEWFSDLGLRWYALPAVSSMLFDVGGIQFTATTFSGWYMSTEIGSRNLCDTNRRNMLETVALKMQLDTRTPTSLWKDKAVVEMNIAVLHSYQSRNVTIVDHHTASESFMKHFENESKLRNGCPADWIWIVPPLSGSITPVFHQEMALYYLKPSFEYQDPAWRTHVWKKGRGESKGKKPRRKFNFKQIARAVKFTSKLFGRALSKRIKATVLYATETGKSEQYAKQLCELLGHAFNAQIYCMSDYDISSIEHEALLIVVASTFGNGDPPENGELFSQELYAMRVQESSEHGLQDSSIGSSKSFMKASSRQEFMKLPLQQVKRIDRWDSLRGSTSDTFTEETFGPLSNVRFAVFALGSSAYPNFCAFGQYVDNILGELGGERLLRVAYGDEMCGQEQSFRKWAPEVFKLACETFCLDPEESLSDASLALQNDSLTVNTVRLVPSANKGSLDSSLSKYHNKKVHCCKAKAKPHNLTRLSEGAKTTMLLEICAPGLEYEPGDHVGIFPANRTELVDGLLNRLVGVDNPDEVLQLQLLKEKQTSNGIFKCWEPHDKIPPDTLRNLLARFFDLTTPPSRQLLTLLAGFCEDTADKERLELLVNDSSAYEDWRHWRLPHLLDVLEEFPSCRPPAPLLLAQLTPLQPRFYSISSSPRRVSDEIHLTVAIVKYRCEDGQGDERYGVCSNYLSGLRADDELFMFVRSALGFHLPSDRSRPIILIGPGTGIAPFRSFWQEFQVLSDLDPTAKLPKMWLFFGCRNRDVDLYAEEKAELQKDQILDRVFLALSREQAIPKTYVQDLIEQEFDSLYQLIVQERGHIYVCGDVTMAEHVYQTIRKCIAGKEQKSEAEVETFLLTLRDESRYHEDIFGITLRTAEIHTKSRATARIRMASQP

Target ORF information:

RefSeq Version	NM_001032072.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nitric oxide synthase, transcript variant K (Nos), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001032072.2

ORF Insert Sequence:

ATGAACATTG GCAATGCGGC GGTGGAGGCC AGGAAATCCG ATCTGATCCT CGAACACGCC 
AAGGACTTCC TCGAGCAGTA CTTTACATCG ATAAAGCGTA CATCATCTAC CGCCCACGAG 
ACGCGATGGA AACAGGTGCG CCAGAGCATT GAGACCACTG GACATTATCA GCTAACCGAA 
ACGGAACTAA TTTATGGTGC CAAATTGGCC TGGCGCAATT CTTCACGTTG CATTGGCCGA 
ATACAATGGT CGAAGTTGCA GGTCTTTGAC TGTCGGTATG TGACAACAAC AAGTGGCATG 
TTTGAAGCCA TTTGCAATCA CATTAAATAT GCAACAAATA AGGGCAACCT GAGATCGGCC 
ATCACGATAT TTCCACAACG CACAGATGCC AAGCATGATT ATCGCATTTG GAATAACCAA 
TTAATATCTT ATGCCGGCTA CAAGCAGGCG GATGGGAAAA TCATTGGCGA TCCCATGAAT 
GTGGAGTTTA CAGAGGTCTG CACCAAGCTG GGCTGGAAGA GCAAGGGCAG CGAGTGGGAC 
ATACTGCCAT TGGTGGTCTC GGCCAATGGT CACGATCCGG ACTACTTTGA TTACCCGCCC 
GAATTGATAC TGGAAGTTCC GCTGACCCAT CCAAAATTCG AATGGTTCTC GGATCTGGGA 
CTGCGATGGT ACGCCCTGCC CGCCGTATCC AGTATGCTGT TCGATGTGGG CGGCATTCAG 
TTTACGGCCA CCACATTCAG TGGTTGGTAC ATGTCGACAG AGATTGGCAG CCGGAATTTA 
TGCGACACAA ATCGCCGCAA TATGCTGGAG ACGGTGGCGC TGAAGATGCA ACTGGACACC 
CGTACGCCCA CATCCTTGTG GAAGGACAAG GCTGTGGTGG AGATGAACAT TGCCGTGCTC 
CACTCCTACC AGAGTCGCAA CGTGACCATT GTGGATCACC ACACGGCCAG CGAGAGCTTT 
ATGAAGCATT TCGAGAACGA GTCCAAGCTC AGGAATGGGT GTCCCGCTGA TTGGATTTGG 
ATCGTGCCGC CGCTGTCGGG CTCCATAACG CCGGTATTCC ATCAGGAGAT GGCTCTGTAC 
TACCTGAAGC CCTCGTTCGA GTACCAGGAT CCCGCCTGGC GAACCCACGT GTGGAAGAAG 
GGGCGTGGCG AGAGCAAGGG CAAGAAGCCA AGACGTAAAT TCAATTTTAA ACAAATCGCT 
AGGGCTGTGA AATTTACATC GAAACTATTT GGACGCGCCT TATCGAAACG CATAAAGGCA 
ACAGTTCTAT ATGCCACCGA AACTGGCAAA TCGGAGCAAT ATGCGAAGCA ACTTTGTGAA 
CTCCTAGGGC ACGCATTCAA TGCACAGATA TATTGCATGT CCGACTACGA TATATCCTCC 
ATTGAGCACG AGGCATTGTT AATTGTTGTG GCCTCCACCT TTGGCAACGG TGATCCGCCC 
GAAAACGGCG AGCTTTTCTC CCAGGAATTG TATGCGATGC GTGTCCAGGA GTCTTCCGAG 
CATGGATTGC AGGACTCCAG CATTGGCTCG TCAAAGTCCT TCATGAAGGC CAGCTCGCGG 
CAGGAGTTCA TGAAGCTGCC ACTGCAACAG GTGAAGAGAA TCGACCGCTG GGACTCGCTG 
CGGGGCTCCA CCTCGGACAC CTTCACCGAG GAGACCTTTG GCCCCCTCTC CAATGTCCGG 
TTCGCCGTTT TTGCCCTCGG CTCCTCGGCC TATCCAAATT TCTGCGCCTT CGGTCAGTAT 
GTGGACAACA TTCTGGGCGA GCTGGGCGGC GAACGCCTGC TGAGGGTGGC CTACGGCGAC 
GAGATGTGCG GACAGGAGCA GTCGTTCCGG AAGTGGGCGC CCGAGGTATT CAAGTTGGCC 
TGCGAGACCT TCTGCCTGGA TCCAGAGGAG AGCCTTTCGG ATGCCTCGCT AGCCCTGCAG 
AACGATTCGC TGACTGTGAA TACGGTGCGC CTGGTGCCGT CGGCGAATAA GGGATCCCTG 
GACAGCAGTT TATCCAAGTA CCACAACAAG AAGGTGCACT GCTGCAAGGC GAAGGCGAAG 
CCCCACAATT TGACCCGTTT GAGTGAGGGA GCCAAGACAA CGATGCTGCT GGAGATCTGT 
GCACCTGGCT TGGAGTACGA GCCGGGTGAT CATGTGGGCA TCTTTCCGGC GAATCGAACG 
GAACTGGTCG ACGGACTGCT AAATCGACTG GTGGGTGTGG ATAATCCCGA CGAGGTGCTG 
CAGTTGCAAT TGCTAAAGGA AAAGCAGACA TCGAATGGTA TATTCAAGTG CTGGGAGCCG 
CACGACAAAA TACCGCCGGA TACTCTAAGG AATCTTCTGG CCCGATTCTT TGATCTGACC 
ACTCCGCCAT CGCGACAGTT GCTCACCCTG CTGGCAGGAT TCTGTGAGGA TACCGCGGAC 
AAGGAGCGGC TGGAGTTGCT GGTCAACGAT TCGTCGGCCT ACGAGGACTG GCGGCACTGG 
CGGCTGCCCC ATCTGCTGGA CGTTCTCGAG GAGTTCCCCT CGTGCCGACC ACCGGCTCCC 
CTTCTGCTTG CCCAACTAAC GCCGCTGCAG CCTCGCTTCT ATTCCATTTC TTCGTCGCCG 
CGCCGCGTTA GTGACGAAAT CCACCTCACG GTGGCCATCG TGAAGTACCG CTGTGAAGAT 
GGCCAGGGAG ACGAGCGGTA TGGCGTGTGC TCTAACTATC TATCCGGCTT GCGGGCAGAC 
GACGAGCTGT TCATGTTCGT GAGGAGCGCC TTGGGCTTCC ATTTGCCCAG CGATCGGAGT 
CGTCCCATTA TTCTGATTGG TCCTGGCACA GGAATAGCTC CATTCCGCTC CTTTTGGCAG 
GAGTTCCAGG TGCTAAGCGA CCTTGATCCC ACGGCCAAAT TGCCCAAGAT GTGGCTCTTC 
TTTGGCTGCC GTAATCGGGA TGTCGATTTG TACGCCGAGG AGAAGGCAGA GCTACAGAAG 
GATCAAATCC TAGACCGAGT TTTTCTCGCT CTGTCCAGGG AGCAGGCCAT TCCGAAGACA 
TATGTGCAGG ACCTGATTGA GCAGGAATTC GATTCGTTGT ACCAGTTGAT TGTCCAGGAG 
CGGGGCCACA TCTACGTCTG CGGCGATGTC ACAATGGCCG AGCATGTGTA CCAGACCATC 
AGGAAGTGCA TTGCCGGCAA AGAGCAGAAA AGCGAGGCGG AAGTTGAGAC ATTTTTGCTA 
ACACTGCGGG ACGAAAGTCG CTACCACGAG GACATCTTTG GCATCACGCT GCGAACGGCT 
GAGATACACA CAAAGTCAAG GGCCACGGCC AGGATACGAA TGGCCTCCCA GCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nos ( NM_001032069.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001027240.1 Drosophila melanogaster nitric oxide synthase, transcript variant F (Nos), mRNA.

Price & Availability↑

CloneID	OFa14413
Clone ID Related Accession (Same CDS sequence)	NM_001032069.2
Accession Version	NM_001032069.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2271bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nitric oxide synthase, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001032069.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGCAGC ATTTCACATC GATATTTGAG AACCTGCGAT TCGTGACCAT CAAACGTTCG 
ACAAATGCGC AACAGCAACA GCAGCAGCAG CAACAGCAAC TGCAGCAGCA GCAGCAGCAG 
CTGCAGCAAC AGAAGGCACA GACACAGCAA CAAAATAGCA GAAAAATCAA AACTCAAGCA 
ACGCCAACGT TGAATGGCAA TGGGCTCTTG AGCGGCAATC CAAATGGCGG AGGCGGTGAC 
TCCTCGCCCA GCCATGAAGT GGACCATCCG GGTGGAGCAC AAGGAGCTCA AGCAGCAGGA 
GGCTTGCCAT CTTCAAGTGG CACGCCATTG AGGCACCACA AGCGCGCCAG TATCTCCACA 
GCATCGCCTC CAATCCGCGA ACGGCGTGGC ACCAACACCA GCATCGTGGT CGAACTGGAT 
GGCAGTGGCA GCGGGAGTGG GAGTGGCGGT GGTGGCGTTG GCGTTGGTCA GGGTGCGGGT 
TGTCCTCCCT CGGGCAGCTG CACTGCGTCC GGAAAAAGTT CGCGGGAACT ATCGCCGTCG 
CCGAAAAACC AACAGCAGCC CAGAAAGATG TCACAGGATT ATCGGTCGCG TGCCGGCAGT 
TTTATGCACC TGGACGACGA GGGACGCAGT CTGCTGATGC GCAAGCCGAT GAGACTGAAG 
AACATCGAGG GCAGGCCGGA GGTCTACGAC ACGCTGCACT GCAAGGGTCG CGAGATTCTT 
TCCTGCTCGA AGGCCACCTG TACGAGCAGC ATTATGAACA TTGGCAATGC GGCGGTGGAG 
GCCAGGAAAT CCGATCTGAT CCTCGAACAC GCCAAGGACT TCCTCGAGCA GTACTTTACA 
TCGATAAAGC GTACATCATC TACCGCCCAC GAGACGCGAT GGAAACAGGT GCGCCAGAGC 
ATTGAGACCA CTGGACATTA TCAGCTAACC GAAACGGAAC TAATTTATGG TGCCAAATTG 
GCCTGGCGCA ATTCTTCACG TTGCATTGGC CGAATACAAT GGTCGAAGTT GCAGGTCTTT 
GACTGTCGGT ATGTGACAAC AACAAGTGGC ATGTTTGAAG CCATTTGCAA TCACATTAAA 
TATGCAACAA ATAAGGGCAA CCTGAGATCG GCCATCACGA TATTTCCACA ACGCACAGAT 
GCCAAGCATG ATTATCGCAT TTGGAATAAC CAATTAATAT CTTATGCCGG CTACAAGCAG 
GCGGATGGGA AAATCATTGG CGATCCCATG AATGTGGAGT TTACAGAGGT CTGCACCAAG 
CTGGGCTGGA AGAGCAAGGG CAGCGAGTGG GACATACTGC CATTGGTGGT CTCGGCCAAT 
GGTCACGATC CGGACTACTT TGATTACCCG CCCGAATTGA TACTGGAAGT TCCGCTGACC 
CATCCAAAAT TCGAATGGTT CTCGGATCTG GGACTGCGAT GGTACGCCCT GCCCGCCGTA 
TCCAGTATGC TGTTCGATGT GGGCGGCATT CAGTTTACGG CCACCACATT CAGTGGTTGG 
TACATGTCGA CAGAGATTGG CAGCCGGAAT TTATGCGACA CAAATCGCCG CAATATGCTG 
GAGACGGTGG CGCTGAAGAT GCAACTGGAC ACCCGTACGC CCACATCCTT GTGGAAGGAC 
AAGGCTGTGG TGGAGATGAA CATTGCCGTG CTCCACTCCT ACCAGAGTCG CAACGTGACC 
ATTGTGGATC ACCACACGGC CAGCGAGAGC TTTATGAAGC ATTTCGAGAA CGAGTCCAAG 
CTCAGGAATG GGTGTCCCGC TGATTGGATT TGGATCGTGC CGCCGCTGTC GGGCTCCATA 
ACGCCGGTAT TCCATCAGGA GATGGCTCTG TACTACCTGA AGCCCTCGTT CGAGTACCAG 
GATCCCGCCT GGCGAACCCA CGTGTGGAAG AAGGGGCGTG GCGAGAGCAA GGGCAAGAAG 
CCAAGACGTA AATTCAATTT TAAACAAATC GCTAGGGCTG TGAAATTTAC ATCGAAACTA 
TTTGGACGCG CCTTATCGAA ACGCATAAAG GCAACAGTTC TATATGCCAC CGAAACTGGC 
AAATCGGAGC AATATGCGAA GCAACTTTGT GAACTCCTAG GGCACGCATT CAATGCACAG 
ATATATTGCA TGTCCGACTA CGATATATCC TCCATTGAGC ACGAGGCATT GTTAATTGTT 
GTGGCCTCCA CCTTTGGCAA CGGTGATCCG CCCGAAAACG GCGAGGTAAG TACTCCCCCC 
CGCAAGGATC ATACTGAACT AATTAATGGA TTAGGCCCAG CCGCCTTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001027240.1
CDS	402..2672
Translation	MSQHFTSIFENLRFVTIKRSTNAQQQQQQQQQQLQQQQQQLQQQKAQTQQQNSRKIKTQATPTLNGNGLLSGNPNGGGGDSSPSHEVDHPGGAQGAQAAGGLPSSSGTPLRHHKRASISTASPPIRERRGTNTSIVVELDGSGSGSGSGGGGVGVGQGAGCPPSGSCTASGKSSRELSPSPKNQQQPRKMSQDYRSRAGSFMHLDDEGRSLLMRKPMRLKNIEGRPEVYDTLHCKGREILSCSKATCTSSIMNIGNAAVEARKSDLILEHAKDFLEQYFTSIKRTSSTAHETRWKQVRQSIETTGHYQLTETELIYGAKLAWRNSSRCIGRIQWSKLQVFDCRYVTTTSGMFEAICNHIKYATNKGNLRSAITIFPQRTDAKHDYRIWNNQLISYAGYKQADGKIIGDPMNVEFTEVCTKLGWKSKGSEWDILPLVVSANGHDPDYFDYPPELILEVPLTHPKFEWFSDLGLRWYALPAVSSMLFDVGGIQFTATTFSGWYMSTEIGSRNLCDTNRRNMLETVALKMQLDTRTPTSLWKDKAVVEMNIAVLHSYQSRNVTIVDHHTASESFMKHFENESKLRNGCPADWIWIVPPLSGSITPVFHQEMALYYLKPSFEYQDPAWRTHVWKKGRGESKGKKPRRKFNFKQIARAVKFTSKLFGRALSKRIKATVLYATETGKSEQYAKQLCELLGHAFNAQIYCMSDYDISSIEHEALLIVVASTFGNGDPPENGEVSTPPRKDHTELINGLGPAAF

Target ORF information:

RefSeq Version	NM_001032069.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nitric oxide synthase, transcript variant F (Nos), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001032069.2

ORF Insert Sequence:

ATGTCGCAGC ATTTCACATC GATATTTGAG AACCTGCGAT TCGTGACCAT CAAACGTTCG 
ACAAATGCGC AACAGCAACA GCAGCAGCAG CAACAGCAAC TGCAGCAGCA GCAGCAGCAG 
CTGCAGCAAC AGAAGGCACA GACACAGCAA CAAAATAGCA GAAAAATCAA AACTCAAGCA 
ACGCCAACGT TGAATGGCAA TGGGCTCTTG AGCGGCAATC CAAATGGCGG AGGCGGTGAC 
TCCTCGCCCA GCCATGAAGT GGACCATCCG GGTGGAGCAC AAGGAGCTCA AGCAGCAGGA 
GGCTTGCCAT CTTCAAGTGG CACGCCATTG AGGCACCACA AGCGCGCCAG TATCTCCACA 
GCATCGCCTC CAATCCGCGA ACGGCGTGGC ACCAACACCA GCATCGTGGT CGAACTGGAT 
GGCAGTGGCA GCGGGAGTGG GAGTGGCGGT GGTGGCGTTG GCGTTGGTCA GGGTGCGGGT 
TGTCCTCCCT CGGGCAGCTG CACTGCGTCC GGAAAAAGTT CGCGGGAACT ATCGCCGTCG 
CCGAAAAACC AACAGCAGCC CAGAAAGATG TCACAGGATT ATCGGTCGCG TGCCGGCAGT 
TTTATGCACC TGGACGACGA GGGACGCAGT CTGCTGATGC GCAAGCCGAT GAGACTGAAG 
AACATCGAGG GCAGGCCGGA GGTCTACGAC ACGCTGCACT GCAAGGGTCG CGAGATTCTT 
TCCTGCTCGA AGGCCACCTG TACGAGCAGC ATTATGAACA TTGGCAATGC GGCGGTGGAG 
GCCAGGAAAT CCGATCTGAT CCTCGAACAC GCCAAGGACT TCCTCGAGCA GTACTTTACA 
TCGATAAAGC GTACATCATC TACCGCCCAC GAGACGCGAT GGAAACAGGT GCGCCAGAGC 
ATTGAGACCA CTGGACATTA TCAGCTAACC GAAACGGAAC TAATTTATGG TGCCAAATTG 
GCCTGGCGCA ATTCTTCACG TTGCATTGGC CGAATACAAT GGTCGAAGTT GCAGGTCTTT 
GACTGTCGGT ATGTGACAAC AACAAGTGGC ATGTTTGAAG CCATTTGCAA TCACATTAAA 
TATGCAACAA ATAAGGGCAA CCTGAGATCG GCCATCACGA TATTTCCACA ACGCACAGAT 
GCCAAGCATG ATTATCGCAT TTGGAATAAC CAATTAATAT CTTATGCCGG CTACAAGCAG 
GCGGATGGGA AAATCATTGG CGATCCCATG AATGTGGAGT TTACAGAGGT CTGCACCAAG 
CTGGGCTGGA AGAGCAAGGG CAGCGAGTGG GACATACTGC CATTGGTGGT CTCGGCCAAT 
GGTCACGATC CGGACTACTT TGATTACCCG CCCGAATTGA TACTGGAAGT TCCGCTGACC 
CATCCAAAAT TCGAATGGTT CTCGGATCTG GGACTGCGAT GGTACGCCCT GCCCGCCGTA 
TCCAGTATGC TGTTCGATGT GGGCGGCATT CAGTTTACGG CCACCACATT CAGTGGTTGG 
TACATGTCGA CAGAGATTGG CAGCCGGAAT TTATGCGACA CAAATCGCCG CAATATGCTG 
GAGACGGTGG CGCTGAAGAT GCAACTGGAC ACCCGTACGC CCACATCCTT GTGGAAGGAC 
AAGGCTGTGG TGGAGATGAA CATTGCCGTG CTCCACTCCT ACCAGAGTCG CAACGTGACC 
ATTGTGGATC ACCACACGGC CAGCGAGAGC TTTATGAAGC ATTTCGAGAA CGAGTCCAAG 
CTCAGGAATG GGTGTCCCGC TGATTGGATT TGGATCGTGC CGCCGCTGTC GGGCTCCATA 
ACGCCGGTAT TCCATCAGGA GATGGCTCTG TACTACCTGA AGCCCTCGTT CGAGTACCAG 
GATCCCGCCT GGCGAACCCA CGTGTGGAAG AAGGGGCGTG GCGAGAGCAA GGGCAAGAAG 
CCAAGACGTA AATTCAATTT TAAACAAATC GCTAGGGCTG TGAAATTTAC ATCGAAACTA 
TTTGGACGCG CCTTATCGAA ACGCATAAAG GCAACAGTTC TATATGCCAC CGAAACTGGC 
AAATCGGAGC AATATGCGAA GCAACTTTGT GAACTCCTAG GGCACGCATT CAATGCACAG 
ATATATTGCA TGTCCGACTA CGATATATCC TCCATTGAGC ACGAGGCATT GTTAATTGTT 
GTGGCCTCCA CCTTTGGCAA CGGTGATCCG CCCGAAAACG GCGAGGTAAG TACTCCCCCC 
CGCAAGGATC ATACTGAACT AATTAATGGA TTAGGCCCAG CCGCCTTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nos ( NM_078817.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523541.2 Drosophila melanogaster nitric oxide synthase, transcript variant A (Nos), mRNA.

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CloneID	OFa14414
Clone ID Related Accession (Same CDS sequence)	NM_078817.4
Accession Version	NM_078817.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4050bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nitric oxide synthase, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_078817.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGCAGC ATTTCACATC GATATTTGAG AACCTGCGAT TCGTGACCAT CAAACGTTCG 
ACAAATGCGC AACAGCAACA GCAGCAGCAG CAACAGCAAC TGCAGCAGCA GCAGCAGCAG 
CTGCAGCAAC AGAAGGCACA GACACAGCAA CAAAATAGCA GAAAAATCAA AACTCAAGCA 
ACGCCAACGT TGAATGGCAA TGGGCTCTTG AGCGGCAATC CAAATGGCGG AGGCGGTGAC 
TCCTCGCCCA GCCATGAAGT GGACCATCCG GGTGGAGCAC AAGGAGCTCA AGCAGCAGGA 
GGCTTGCCAT CTTCAAGTGG CACGCCATTG AGGCACCACA AGCGCGCCAG TATCTCCACA 
GCATCGCCTC CAATCCGCGA ACGGCGTGGC ACCAACACCA GCATCGTGGT CGAACTGGAT 
GGCAGTGGCA GCGGGAGTGG GAGTGGCGGT GGTGGCGTTG GCGTTGGTCA GGGTGCGGGT 
TGTCCTCCCT CGGGCAGCTG CACTGCGTCC GGAAAAAGTT CGCGGGAACT ATCGCCGTCG 
CCGAAAAACC AACAGCAGCC CAGAAAGATG TCACAGGATT ATCGGTCGCG TGCCGGCAGT 
TTTATGCACC TGGACGACGA GGGACGCAGT CTGCTGATGC GCAAGCCGAT GAGACTGAAG 
AACATCGAGG GCAGGCCGGA GGTCTACGAC ACGCTGCACT GCAAGGGTCG CGAGATTCTT 
TCCTGCTCGA AGGCCACCTG TACGAGCAGC ATTATGAACA TTGGCAATGC GGCGGTGGAG 
GCCAGGAAAT CCGATCTGAT CCTCGAACAC GCCAAGGACT TCCTCGAGCA GTACTTTACA 
TCGATAAAGC GTACATCATC TACCGCCCAC GAGACGCGAT GGAAACAGGT GCGCCAGAGC 
ATTGAGACCA CTGGACATTA TCAGCTAACC GAAACGGAAC TAATTTATGG TGCCAAATTG 
GCCTGGCGCA ATTCTTCACG TTGCATTGGC CGAATACAAT GGTCGAAGTT GCAGGTCTTT 
GACTGTCGGT ATGTGACAAC AACAAGTGGC ATGTTTGAAG CCATTTGCAA TCACATTAAA 
TATGCAACAA ATAAGGGCAA CCTGAGATCG GCCATCACGA TATTTCCACA ACGCACAGAT 
GCCAAGCATG ATTATCGCAT TTGGAATAAC CAATTAATAT CTTATGCCGG CTACAAGCAG 
GCGGATGGGA AAATCATTGG CGATCCCATG AATGTGGAGT TTACAGAGGT CTGCACCAAG 
CTGGGCTGGA AGAGCAAGGG CAGCGAGTGG GACATACTGC CATTGGTGGT CTCGGCCAAT 
GGTCACGATC CGGACTACTT TGATTACCCG CCCGAATTGA TACTGGAAGT TCCGCTGACC 
CATCCAAAAT TCGAATGGTT CTCGGATCTG GGACTGCGAT GGTACGCCCT GCCCGCCGTA 
TCCAGTATGC TGTTCGATGT GGGCGGCATT CAGTTTACGG CCACCACATT CAGTGGTTGG 
TACATGTCGA CAGAGATTGG CAGCCGGAAT TTATGCGACA CAAATCGCCG CAATATGCTG 
GAGACGGTGG CGCTGAAGAT GCAACTGGAC ACCCGTACGC CCACATCCTT GTGGAAGGAC 
AAGGCTGTGG TGGAGATGAA CATTGCCGTG CTCCACTCCT ACCAGAGTCG CAACGTGACC 
ATTGTGGATC ACCACACGGC CAGCGAGAGC TTTATGAAGC ATTTCGAGAA CGAGTCCAAG 
CTCAGGAATG GGTGTCCCGC TGATTGGATT TGGATCGTGC CGCCGCTGTC GGGCTCCATA 
ACGCCGGTAT TCCATCAGGA GATGGCTCTG TACTACCTGA AGCCCTCGTT CGAGTACCAG 
GATCCCGCCT GGCGAACCCA CGTGTGGAAG AAGGGGCGTG GCGAGAGCAA GGGCAAGAAG 
CCAAGACGTA AATTCAATTT TAAACAAATC GCTAGGGCTG TGAAATTTAC ATCGAAACTA 
TTTGGACGCG CCTTATCGAA ACGCATAAAG GCAACAGTTC TATATGCCAC CGAAACTGGC 
AAATCGGAGC AATATGCGAA GCAACTTTGT GAACTCCTAG GGCACGCATT CAATGCACAG 
ATATATTGCA TGTCCGACTA CGATATATCC TCCATTGAGC ACGAGGCATT GTTAATTGTT 
GTGGCCTCCA CCTTTGGCAA CGGTGATCCG CCCGAAAACG GCGAGCTTTT CTCCCAGGAA 
TTGTATGCGA TGCGTGTCCA GGAGTCTTCC GAGCATGGAT TGCAGGACTC CAGCATTGGC 
TCGTCAAAGT CCTTCATGAA GGCCAGCTCG CGGCAGGAGT TCATGAAGCT GCCACTGCAA 
CAGGTGAAGA GAATCGACCG CTGGGACTCG CTGCGGGGCT CCACCTCGGA CACCTTCACC 
GAGGAGACCT TTGGCCCCCT CTCCAATGTC CGGTTCGCCG TTTTTGCCCT CGGCTCCTCG 
GCCTATCCAA ATTTCTGCGC CTTCGGTCAG TATGTGGACA ACATTCTGGG CGAGCTGGGC 
GGCGAACGCC TGCTGAGGGT GGCCTACGGC GACGAGATGT GCGGACAGGA GCAGTCGTTC 
CGGAAGTGGG CGCCCGAGGT ATTCAAGTTG GCCTGCGAGA CCTTCTGCCT GGATCCAGAG 
GAGAGCCTTT CGGATGCCTC GCTAGCCCTG CAGAACGATT CGCTGACTGT GAATACGGTG 
CGCCTGGTGC CGTCGGCGAA TAAGGGATCC CTGGACAGCA GTTTATCCAA GTACCACAAC 
AAGAAGGTGC ACTGCTGCAA GGCGAAGGCG AAGCCCCACA ATTTGACCCG TTTGAGTGAG 
GGAGCCAAGA CAACGATGCT GCTGGAGATC TGTGCACCTG GCTTGGAGTA CGAGCCGGGT 
GATCATGTGG GCATCTTTCC GGCGAATCGA ACGGAACTGG TCGACGGACT GCTAAATCGA 
CTGGTGGGTG TGGATAATCC CGACGAGGTG CTGCAGTTGC AATTGCTAAA GGAAAAGCAG 
ACATCGAATG GTATATTCAA GTGCTGGGAG CCGCACGACA AAATACCGCC GGATACTCTA 
AGGAATCTTC TGGCCCGATT CTTTGATCTG ACCACTCCGC CATCGCGACA GTTGCTCACC 
CTGCTGGCAG GATTCTGTGA GGATACCGCG GACAAGGAGC GGCTGGAGTT GCTGGTCAAC 
GATTCGTCGG CCTACGAGGA CTGGCGGCAC TGGCGGCTGC CCCATCTGCT GGACGTTCTC 
GAGGAGTTCC CCTCGTGCCG ACCACCGGCT CCCCTTCTGC TTGCCCAACT AACGCCGCTG 
CAGCCTCGCT TCTATTCCAT TTCTTCGTCG CCGCGCCGCG TTAGTGACGA AATCCACCTC 
ACGGTGGCCA TCGTGAAGTA CCGCTGTGAA GATGGCCAGG GAGACGAGCG GTATGGCGTG 
TGCTCTAACT ATCTATCCGG CTTGCGGGCA GACGACGAGC TGTTCATGTT CGTGAGGAGC 
GCCTTGGGCT TCCATTTGCC CAGCGATCGG AGTCGTCCCA TTATTCTGAT TGGTCCTGGC 
ACAGGAATAG CTCCATTCCG CTCCTTTTGG CAGGAGTTCC AGGTGCTAAG CGACCTTGAT 
CCCACGGCCA AATTGCCCAA GATGTGGCTC TTCTTTGGCT GCCGTAATCG GGATGTCGAT 
TTGTACGCCG AGGAGAAGGC AGAGCTACAG AAGGATCAAA TCCTAGACCG AGTTTTTCTC 
GCTCTGTCCA GGGAGCAGGC CATTCCGAAG ACATATGTGC AGGACCTGAT TGAGCAGGAA 
TTCGATTCGT TGTACCAGTT GATTGTCCAG GAGCGGGGCC ACATCTACGT CTGCGGCGAT 
GTCACAATGG CCGAGCATGT GTACCAGACC ATCAGGAAGT GCATTGCCGG CAAAGAGCAG 
AAAAGCGAGG CGGAAGTTGA GACATTTTTG CTAACACTGC GGGACGAAAG TCGCTACCAC 
GAGGACATCT TTGGCATCAC GCTGCGAACG GCTGAGATAC ACACAAAGTC AAGGGCCACG 
GCCAGGATAC GAATGGCCTC CCAGCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523541.2
CDS	402..4451
Translation	MSQHFTSIFENLRFVTIKRSTNAQQQQQQQQQQLQQQQQQLQQQKAQTQQQNSRKIKTQATPTLNGNGLLSGNPNGGGGDSSPSHEVDHPGGAQGAQAAGGLPSSSGTPLRHHKRASISTASPPIRERRGTNTSIVVELDGSGSGSGSGGGGVGVGQGAGCPPSGSCTASGKSSRELSPSPKNQQQPRKMSQDYRSRAGSFMHLDDEGRSLLMRKPMRLKNIEGRPEVYDTLHCKGREILSCSKATCTSSIMNIGNAAVEARKSDLILEHAKDFLEQYFTSIKRTSSTAHETRWKQVRQSIETTGHYQLTETELIYGAKLAWRNSSRCIGRIQWSKLQVFDCRYVTTTSGMFEAICNHIKYATNKGNLRSAITIFPQRTDAKHDYRIWNNQLISYAGYKQADGKIIGDPMNVEFTEVCTKLGWKSKGSEWDILPLVVSANGHDPDYFDYPPELILEVPLTHPKFEWFSDLGLRWYALPAVSSMLFDVGGIQFTATTFSGWYMSTEIGSRNLCDTNRRNMLETVALKMQLDTRTPTSLWKDKAVVEMNIAVLHSYQSRNVTIVDHHTASESFMKHFENESKLRNGCPADWIWIVPPLSGSITPVFHQEMALYYLKPSFEYQDPAWRTHVWKKGRGESKGKKPRRKFNFKQIARAVKFTSKLFGRALSKRIKATVLYATETGKSEQYAKQLCELLGHAFNAQIYCMSDYDISSIEHEALLIVVASTFGNGDPPENGELFSQELYAMRVQESSEHGLQDSSIGSSKSFMKASSRQEFMKLPLQQVKRIDRWDSLRGSTSDTFTEETFGPLSNVRFAVFALGSSAYPNFCAFGQYVDNILGELGGERLLRVAYGDEMCGQEQSFRKWAPEVFKLACETFCLDPEESLSDASLALQNDSLTVNTVRLVPSANKGSLDSSLSKYHNKKVHCCKAKAKPHNLTRLSEGAKTTMLLEICAPGLEYEPGDHVGIFPANRTELVDGLLNRLVGVDNPDEVLQLQLLKEKQTSNGIFKCWEPHDKIPPDTLRNLLARFFDLTTPPSRQLLTLLAGFCEDTADKERLELLVNDSSAYEDWRHWRLPHLLDVLEEFPSCRPPAPLLLAQLTPLQPRFYSISSSPRRVSDEIHLTVAIVKYRCEDGQGDERYGVCSNYLSGLRADDELFMFVRSALGFHLPSDRSRPIILIGPGTGIAPFRSFWQEFQVLSDLDPTAKLPKMWLFFGCRNRDVDLYAEEKAELQKDQILDRVFLALSREQAIPKTYVQDLIEQEFDSLYQLIVQERGHIYVCGDVTMAEHVYQTIRKCIAGKEQKSEAEVETFLLTLRDESRYHEDIFGITLRTAEIHTKSRATARIRMASQP

Target ORF information:

RefSeq Version	NM_078817.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nitric oxide synthase, transcript variant A (Nos), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078817.4

ORF Insert Sequence:

ATGTCGCAGC ATTTCACATC GATATTTGAG AACCTGCGAT TCGTGACCAT CAAACGTTCG 
ACAAATGCGC AACAGCAACA GCAGCAGCAG CAACAGCAAC TGCAGCAGCA GCAGCAGCAG 
CTGCAGCAAC AGAAGGCACA GACACAGCAA CAAAATAGCA GAAAAATCAA AACTCAAGCA 
ACGCCAACGT TGAATGGCAA TGGGCTCTTG AGCGGCAATC CAAATGGCGG AGGCGGTGAC 
TCCTCGCCCA GCCATGAAGT GGACCATCCG GGTGGAGCAC AAGGAGCTCA AGCAGCAGGA 
GGCTTGCCAT CTTCAAGTGG CACGCCATTG AGGCACCACA AGCGCGCCAG TATCTCCACA 
GCATCGCCTC CAATCCGCGA ACGGCGTGGC ACCAACACCA GCATCGTGGT CGAACTGGAT 
GGCAGTGGCA GCGGGAGTGG GAGTGGCGGT GGTGGCGTTG GCGTTGGTCA GGGTGCGGGT 
TGTCCTCCCT CGGGCAGCTG CACTGCGTCC GGAAAAAGTT CGCGGGAACT ATCGCCGTCG 
CCGAAAAACC AACAGCAGCC CAGAAAGATG TCACAGGATT ATCGGTCGCG TGCCGGCAGT 
TTTATGCACC TGGACGACGA GGGACGCAGT CTGCTGATGC GCAAGCCGAT GAGACTGAAG 
AACATCGAGG GCAGGCCGGA GGTCTACGAC ACGCTGCACT GCAAGGGTCG CGAGATTCTT 
TCCTGCTCGA AGGCCACCTG TACGAGCAGC ATTATGAACA TTGGCAATGC GGCGGTGGAG 
GCCAGGAAAT CCGATCTGAT CCTCGAACAC GCCAAGGACT TCCTCGAGCA GTACTTTACA 
TCGATAAAGC GTACATCATC TACCGCCCAC GAGACGCGAT GGAAACAGGT GCGCCAGAGC 
ATTGAGACCA CTGGACATTA TCAGCTAACC GAAACGGAAC TAATTTATGG TGCCAAATTG 
GCCTGGCGCA ATTCTTCACG TTGCATTGGC CGAATACAAT GGTCGAAGTT GCAGGTCTTT 
GACTGTCGGT ATGTGACAAC AACAAGTGGC ATGTTTGAAG CCATTTGCAA TCACATTAAA 
TATGCAACAA ATAAGGGCAA CCTGAGATCG GCCATCACGA TATTTCCACA ACGCACAGAT 
GCCAAGCATG ATTATCGCAT TTGGAATAAC CAATTAATAT CTTATGCCGG CTACAAGCAG 
GCGGATGGGA AAATCATTGG CGATCCCATG AATGTGGAGT TTACAGAGGT CTGCACCAAG 
CTGGGCTGGA AGAGCAAGGG CAGCGAGTGG GACATACTGC CATTGGTGGT CTCGGCCAAT 
GGTCACGATC CGGACTACTT TGATTACCCG CCCGAATTGA TACTGGAAGT TCCGCTGACC 
CATCCAAAAT TCGAATGGTT CTCGGATCTG GGACTGCGAT GGTACGCCCT GCCCGCCGTA 
TCCAGTATGC TGTTCGATGT GGGCGGCATT CAGTTTACGG CCACCACATT CAGTGGTTGG 
TACATGTCGA CAGAGATTGG CAGCCGGAAT TTATGCGACA CAAATCGCCG CAATATGCTG 
GAGACGGTGG CGCTGAAGAT GCAACTGGAC ACCCGTACGC CCACATCCTT GTGGAAGGAC 
AAGGCTGTGG TGGAGATGAA CATTGCCGTG CTCCACTCCT ACCAGAGTCG CAACGTGACC 
ATTGTGGATC ACCACACGGC CAGCGAGAGC TTTATGAAGC ATTTCGAGAA CGAGTCCAAG 
CTCAGGAATG GGTGTCCCGC TGATTGGATT TGGATCGTGC CGCCGCTGTC GGGCTCCATA 
ACGCCGGTAT TCCATCAGGA GATGGCTCTG TACTACCTGA AGCCCTCGTT CGAGTACCAG 
GATCCCGCCT GGCGAACCCA CGTGTGGAAG AAGGGGCGTG GCGAGAGCAA GGGCAAGAAG 
CCAAGACGTA AATTCAATTT TAAACAAATC GCTAGGGCTG TGAAATTTAC ATCGAAACTA 
TTTGGACGCG CCTTATCGAA ACGCATAAAG GCAACAGTTC TATATGCCAC CGAAACTGGC 
AAATCGGAGC AATATGCGAA GCAACTTTGT GAACTCCTAG GGCACGCATT CAATGCACAG 
ATATATTGCA TGTCCGACTA CGATATATCC TCCATTGAGC ACGAGGCATT GTTAATTGTT 
GTGGCCTCCA CCTTTGGCAA CGGTGATCCG CCCGAAAACG GCGAGCTTTT CTCCCAGGAA 
TTGTATGCGA TGCGTGTCCA GGAGTCTTCC GAGCATGGAT TGCAGGACTC CAGCATTGGC 
TCGTCAAAGT CCTTCATGAA GGCCAGCTCG CGGCAGGAGT TCATGAAGCT GCCACTGCAA 
CAGGTGAAGA GAATCGACCG CTGGGACTCG CTGCGGGGCT CCACCTCGGA CACCTTCACC 
GAGGAGACCT TTGGCCCCCT CTCCAATGTC CGGTTCGCCG TTTTTGCCCT CGGCTCCTCG 
GCCTATCCAA ATTTCTGCGC CTTCGGTCAG TATGTGGACA ACATTCTGGG CGAGCTGGGC 
GGCGAACGCC TGCTGAGGGT GGCCTACGGC GACGAGATGT GCGGACAGGA GCAGTCGTTC 
CGGAAGTGGG CGCCCGAGGT ATTCAAGTTG GCCTGCGAGA CCTTCTGCCT GGATCCAGAG 
GAGAGCCTTT CGGATGCCTC GCTAGCCCTG CAGAACGATT CGCTGACTGT GAATACGGTG 
CGCCTGGTGC CGTCGGCGAA TAAGGGATCC CTGGACAGCA GTTTATCCAA GTACCACAAC 
AAGAAGGTGC ACTGCTGCAA GGCGAAGGCG AAGCCCCACA ATTTGACCCG TTTGAGTGAG 
GGAGCCAAGA CAACGATGCT GCTGGAGATC TGTGCACCTG GCTTGGAGTA CGAGCCGGGT 
GATCATGTGG GCATCTTTCC GGCGAATCGA ACGGAACTGG TCGACGGACT GCTAAATCGA 
CTGGTGGGTG TGGATAATCC CGACGAGGTG CTGCAGTTGC AATTGCTAAA GGAAAAGCAG 
ACATCGAATG GTATATTCAA GTGCTGGGAG CCGCACGACA AAATACCGCC GGATACTCTA 
AGGAATCTTC TGGCCCGATT CTTTGATCTG ACCACTCCGC CATCGCGACA GTTGCTCACC 
CTGCTGGCAG GATTCTGTGA GGATACCGCG GACAAGGAGC GGCTGGAGTT GCTGGTCAAC 
GATTCGTCGG CCTACGAGGA CTGGCGGCAC TGGCGGCTGC CCCATCTGCT GGACGTTCTC 
GAGGAGTTCC CCTCGTGCCG ACCACCGGCT CCCCTTCTGC TTGCCCAACT AACGCCGCTG 
CAGCCTCGCT TCTATTCCAT TTCTTCGTCG CCGCGCCGCG TTAGTGACGA AATCCACCTC 
ACGGTGGCCA TCGTGAAGTA CCGCTGTGAA GATGGCCAGG GAGACGAGCG GTATGGCGTG 
TGCTCTAACT ATCTATCCGG CTTGCGGGCA GACGACGAGC TGTTCATGTT CGTGAGGAGC 
GCCTTGGGCT TCCATTTGCC CAGCGATCGG AGTCGTCCCA TTATTCTGAT TGGTCCTGGC 
ACAGGAATAG CTCCATTCCG CTCCTTTTGG CAGGAGTTCC AGGTGCTAAG CGACCTTGAT 
CCCACGGCCA AATTGCCCAA GATGTGGCTC TTCTTTGGCT GCCGTAATCG GGATGTCGAT 
TTGTACGCCG AGGAGAAGGC AGAGCTACAG AAGGATCAAA TCCTAGACCG AGTTTTTCTC 
GCTCTGTCCA GGGAGCAGGC CATTCCGAAG ACATATGTGC AGGACCTGAT TGAGCAGGAA 
TTCGATTCGT TGTACCAGTT GATTGTCCAG GAGCGGGGCC ACATCTACGT CTGCGGCGAT 
GTCACAATGG CCGAGCATGT GTACCAGACC ATCAGGAAGT GCATTGCCGG CAAAGAGCAG 
AAAAGCGAGG CGGAAGTTGA GACATTTTTG CTAACACTGC GGGACGAAAG TCGCTACCAC 
GAGGACATCT TTGGCATCAC GCTGCGAACG GCTGAGATAC ACACAAAGTC AAGGGCCACG 
GCCAGGATAC GAATGGCCTC CCAGCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Nos cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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