Nos1 cDNA ORF clone, Mus musculus(house mouse)

The following Nos1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nos1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu20278 NM_008712.3
Latest version!
Mus musculus nitric oxide synthase 1, neuronal (Nos1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $839.30
$1199.00
OMu20278 XM_017320707.1
Latest version!
Mus musculus nitric oxide synthase 1, neuronal (Nos1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $839.30
$1199.00
OMu35811 XM_006530187.3
Latest version!
Mus musculus nitric oxide synthase 1, neuronal (Nos1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OMu35811 XM_006530188.3
Latest version!
Mus musculus nitric oxide synthase 1, neuronal (Nos1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OMu35811 XM_006530190.1
Latest version!
Mus musculus nitric oxide synthase 1, neuronal (Nos1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OMu35811 XM_006530189.3
Latest version!
Mus musculus nitric oxide synthase 1, neuronal (Nos1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OMu35812 XM_006530191.3
Latest version!
Mus musculus nitric oxide synthase 1, neuronal (Nos1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OMu35813 XM_006530192.3
Latest version!
Mus musculus nitric oxide synthase 1, neuronal (Nos1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu20278
    Clone ID Related Accession (Same CDS sequence) XM_017320707.1 , NM_008712.3
    Accession Version NM_008712.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4290bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-28
    Organism Mus musculus(house mouse)
    Product nitric oxide synthase, brain
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC114617.16 and AC114993.18. On May 5, 2015 this sequence version replaced NM_008712.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAAGAGC ACACGTTTGG GGTCCAGCAG ATCCAACCCA ACGTCATTTC TGTCCGTCTC 
    TTCAAACGCA AAGTGGGAGG TCTGGGCTTC CTGGTGAAGG AACGGGTCAG CAAGCCCCCG
    GTGATCATCT CAGATCTGAT CCGAGGAGGT GCCGCGGAGC AGAGCGGCCT TATCCAAGCC
    GGCGACATCA TTCTCGCCGT CAATGATCGG CCCCTGGTAG ACCTCAGCTA TGACAGCGCT
    CTGGAAGTTC TCAGGGGCAT TGCCTCTGAG ACCCATGTGG TCCTCATTCT GAGAGGCCCT
    GAGGGCTTTA CTACACACCT GGAGACCACC TTCACAGGGG ATGGAACCCC CAAGACCATC
    CGTGTGACCC AGCCCCTGGG GACACCCACC AAAGCTGTCG ATCTGTCTCG CCAGCCATCA
    GCCAGCAAAG ACCAGCCATT AGCAGTAGAC AGGGTCCCAG GTCCCAGTAA CGGACCTCAG
    CATGCCCAAG GCCGTGGACA GGGAGCCGGC TCAGTCTCCC AGGCTAATGG TGTGGCCATT
    GACCCCACAA TGAAAAATAC CAAGGCCAAC CTCCAGGACA GCGGAGAACA GGATGAACTG
    CTCAAAGAGA TAGAACCTGT GTTGAGCATC CTCACCGGTG GGGGAAAAGC AGTCAACAGA
    GGGGGACCAG CCAAAGCAGA GATGAAAGAC ACAGGAATCC AGGTGGACAG AGACCTCGAC
    GGCAAACTGC ACAAAGCTCC GCCCCTGGGC GGGGAGAATG ATCGAGTCTT CAATGATCTG
    TGGGGGAAGG GCAACGTCCC TGTGGTCCTC AACAACCCGT ATTCAGAGAA CGAACAGTCT
    CCCGCCTCGG GCAAACAGTC TCCTACCAAG AATGGCAGTC CTTCCAGGTG CCCTCGCTTC
    CTTAAGGTCA AGAACTGGGA GACAGATGTG GTCCTCACTG ACACCCTGCA CCTGAAGAGC
    ACGCTGGAAA CGGGGTGCAC AGAGCAGATC TGCATGGGCT CCATCATGCT GCCATCCCAT
    CACATACGGA AGTCAGAAGA TGTCCGCACC AAGGACCAGC TCTTCCCTCT AGCCAAAGAA
    TTTCTCGACC AATACTACTC CTCCATTAAG AGGTTTGGCT CCAAGGCCCA CATGGACAGG
    CTGGAGGAGG TGAACAAGGA GATCGAGAGC ACCAGCACCT ACCAGCTCAA GGACACGGAG
    CTCATCTATG GCGCCAAGCA TGCCTGGCGG AACGCCTCTC GCTGTGTGGG CAGGATCCAG
    TGGTCCAAGC TGCAGGTGTT CGATGCCCGA GACTGCACCA CAGCCCATGG GATGTTCAAC
    TACATCTGTA ACCACGTCAA GTACGCCACC AACAAAGGGA ACCTCAGGTC GGCCATCACT
    ATATTCCCTC AAAGGACTGA TGGCAAGCAT GACTTCCGAG TGTGGAACTC GCAGCTCATC
    CGCTATGCCG GCTACAAGCA GCCAGATGGC TCTACCTTGG GCGATCCAGC TAATGTGGAG
    TTCACAGAGA TCTGTATACA GCAGGGCTGG AAACCCCCAA GAGGCCGGTT CGATGTGCTG
    CCTCTCCTGC TCCAGGCCAA TGGCAATGAC CCTGAACTCT TTCAGATCCC CCCAGAGCTG
    GTGCTGGAAG TACCCATCAG GCACCCCAAG TTCGACTGGT TTAAGGACCT GGGGCTCAAA
    TGGTATGGCC TCCCCGCTGT GTCCAACATG CTGCTGGAGA TTGGGGGCCT GGAGTTCAGC
    GCCTGTCCCT TTAGTGGCTG GTACATGGGC ACGGAGATTG GCGTTCGTGA TTACTGTGAC
    AACTCTCGAT ACAACATCCT GGAGGAAGTA GCCAAGAAAA TGGATTTGGA CATGAGGAAG
    ACATCCTCCC TCTGGAAGGA CCAAGCCCTG GTGGAGATTA ACATTGCTGT CCTATACAGC
    TTCCAGAGTG ACAAGGTGAC CATTGTGGAC CACCACTCTG CCACCGAGTC CTTCATCAAA
    CACATGGAGA ATGAGTACCG CTGCAGAGGG GGCTGTCCCG CCGACTGGGT GTGGATTGTG
    CCCCCCATGT CGGGCAGCAT CACCCCTGTC TTCCACCAGG AGATGCTCAA CTACCGGCTC
    ACCCCGTCCT TTGAGTACCA GCCTGATCCA TGGAACACCC ACGTGTGGAA GGGCACCAAT
    GGGACCCCCA CGAAGCGGCG AGCCATTGGC TTCAAGAAAT TGGCAGAGGC CGTCAAGTTC
    TCAGCCAAGC TGATGGGACA GGCCATGGCC AAGAGAGTCA AAGCGACCAT TCTCTACGCC
    ACCGAGACAG GCAAATCCCA AGCCTATGCC AAGACCCTGT GCGAGATCTT CAAGCATGCC
    TTCGATGCCA AGGCTATGTC CATGGAGGAA TATGACATCG TGCACCTGGA GCACGAAGCC
    CTGGTCTTGG TGGTCACCAG CACCTTTGGC AATGGAGACC CCCCAGAGAA TGGGGAGAAA
    TTCGGCTGTG CTTTGATGGA GATGAGGCAC CCCAACTCTG TGCAAGAGGA GAGGAAGAGC
    TACAAGGTCC GATTCAACAG CGTCTCCTCC TATTCTGACT CCCGCAAGTC ATCAGGCGAT
    GGACCAGACC TCAGAGACAA CTTTGAAAGT ACTGGACCGC TGGCCAATGT GAGGTTCTCA
    GTCTTCGGCC TGGGCTCTCG GGCATACCCT CACTTCTGTG CCTTTGGGCA CGCAGTGGAC
    ACTCTCCTGG AGGAGCTGGG CGGGGAGAGG ATTCTGAAGA TGAGGGAGGG AGATGAGCTC
    TGCGGACAGG AAGAGGCTTT CAGGACCTGG GCTAAGAAAG TCTTCAAGGC AGCCTGTGAT
    GTGTTCTGTG TGGGGGATGA CGTCAACATT GAGAAGGCGA ACAATTCCCT CATTAGCAAT
    GACCGCAGCT GGAAGAGGAA TAAATTCCGC CTCACTTATG TGGCAGAAGC TCCAGAGCTG
    ACCCAAGGTC TTTCCAATGT TCACAAAAAG CGAGTCTCAG CCGCCCGACT CCTCAGCCGC
    CAAAACCTGC AAAGTCCTAA ATCCAGCCGA TCGACCATCT TCGTGCGTCT CCACACCAAC
    GGGAATCAGG AGTTGCAGTA CCAGCCAGGG GACCACCTGG GTGTCTTCCC TGGCAACCAT
    GAGGACCTCG TGAATGCACT CATTGAACGG CTGGAGGACG CGCCGCCTGC CAACCACGTG
    GTGAAGGTGG AAATGCTGGA GGAAAGGAAC ACGGCTCTGG GTGTCATCAG TAATTGGAAG
    GATGAATCTC GCCTCCCACC CTGCACCATC TTCCAGGCCT TCAAGTACTA CCTGGACATC
    ACCACGCCGC CCACGCCCCT GCAGCTGCAG CAGTTCGCCT CCCTGGCCAC CAATGAGAAA
    GAGAAGCAGC GGCTGCTGGT CCTCAGCAAG GGGCTTCAGG AATATGAGGA ATGGAAATGG
    GGCAAGAACC CCACAATGGT GGAGGTGCTG GAGGAGTTCC CGTCCATCCA GATGCCGGCC
    ACACTTCTCC TCACACAGCT GTCGCTGCTA CAACCTCGCT ACTATTCCAT CAGCTCCTCT
    CCAGACATGT ACCCCGACGA GGTGCACCTC ACCGTGGCCA TCGTGTCCTA CCATACCCGA
    GATGGAGAAG GACCGGTTCA CCACGGGGTG TGCTCCTCCT GGCTCAACCG AATACAGGCT
    GACGATGTCG TCCCCTGCTT CGTGAGAGGT GCCCCTAGCT TCCACCTGCC TCGAAACCCC
    CAGGTGCCTT GCATCCTGGT TGGCCCAGGC ACCGGCATCG CTCCCTTCCG AAGTTTTTGG
    CAACAGCGAC AATTTGACAT CCAACACAAA GGAATGAATC CGTGCCCCAT GGTTCTGGTC
    TTCGGGTGTC GACAATCCAA GATAGATCAT ATCTACAGAG AGGAGACCCT GCAGGCCAAG
    AACAAGGGCG TCTTCAGAGA GCTGTACACT GCCTACTCCC GGGAACCCGA CAGGCCAAAG
    AAATATGTAC AGGACGTGCT GCAGGAACAG CTGGCCGAGT CTGTGTACCG CGCCCTGAAG
    GAGCAAGGAG GCCATATTTA TGTCTGCGGG GACGTTACCA TGGCCGCTGA TGTCCTCAAA
    GCCATCCAGC GCATAATGAC CCAGCAGGGG AAACTCTCGG AGGAGGATGC TGGTGTGTTC
    ATCAGCAGGC TGAGGGATGA CAACCGGTAC CACGAGGACA TCTTTGGGGT CACCCTCAGA
    ACATATGAAG TGACCAACCG CCTTAGATCT GAGTCCATCG CCTTCATTGA AGAGAGCAAA
    AAAGACACAG ATGAGGTTTT CAGCTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_032738.1
    CDS379..4668
    Translation

    Target ORF information:

    RefSeq Version NM_008712.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus nitric oxide synthase 1, neuronal (Nos1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_008712.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAAGAGC ACACGTTTGG GGTCCAGCAG ATCCAACCCA ACGTCATTTC TGTCCGTCTC 
    TTCAAACGCA AAGTGGGAGG TCTGGGCTTC CTGGTGAAGG AACGGGTCAG CAAGCCCCCG
    GTGATCATCT CAGATCTGAT CCGAGGAGGT GCCGCGGAGC AGAGCGGCCT TATCCAAGCC
    GGCGACATCA TTCTCGCCGT CAATGATCGG CCCCTGGTAG ACCTCAGCTA TGACAGCGCT
    CTGGAAGTTC TCAGGGGCAT TGCCTCTGAG ACCCATGTGG TCCTCATTCT GAGAGGCCCT
    GAGGGCTTTA CTACACACCT GGAGACCACC TTCACAGGGG ATGGAACCCC CAAGACCATC
    CGTGTGACCC AGCCCCTGGG GACACCCACC AAAGCTGTCG ATCTGTCTCG CCAGCCATCA
    GCCAGCAAAG ACCAGCCATT AGCAGTAGAC AGGGTCCCAG GTCCCAGTAA CGGACCTCAG
    CATGCCCAAG GCCGTGGACA GGGAGCCGGC TCAGTCTCCC AGGCTAATGG TGTGGCCATT
    GACCCCACAA TGAAAAATAC CAAGGCCAAC CTCCAGGACA GCGGAGAACA GGATGAACTG
    CTCAAAGAGA TAGAACCTGT GTTGAGCATC CTCACCGGTG GGGGAAAAGC AGTCAACAGA
    GGGGGACCAG CCAAAGCAGA GATGAAAGAC ACAGGAATCC AGGTGGACAG AGACCTCGAC
    GGCAAACTGC ACAAAGCTCC GCCCCTGGGC GGGGAGAATG ATCGAGTCTT CAATGATCTG
    TGGGGGAAGG GCAACGTCCC TGTGGTCCTC AACAACCCGT ATTCAGAGAA CGAACAGTCT
    CCCGCCTCGG GCAAACAGTC TCCTACCAAG AATGGCAGTC CTTCCAGGTG CCCTCGCTTC
    CTTAAGGTCA AGAACTGGGA GACAGATGTG GTCCTCACTG ACACCCTGCA CCTGAAGAGC
    ACGCTGGAAA CGGGGTGCAC AGAGCAGATC TGCATGGGCT CCATCATGCT GCCATCCCAT
    CACATACGGA AGTCAGAAGA TGTCCGCACC AAGGACCAGC TCTTCCCTCT AGCCAAAGAA
    TTTCTCGACC AATACTACTC CTCCATTAAG AGGTTTGGCT CCAAGGCCCA CATGGACAGG
    CTGGAGGAGG TGAACAAGGA GATCGAGAGC ACCAGCACCT ACCAGCTCAA GGACACGGAG
    CTCATCTATG GCGCCAAGCA TGCCTGGCGG AACGCCTCTC GCTGTGTGGG CAGGATCCAG
    TGGTCCAAGC TGCAGGTGTT CGATGCCCGA GACTGCACCA CAGCCCATGG GATGTTCAAC
    TACATCTGTA ACCACGTCAA GTACGCCACC AACAAAGGGA ACCTCAGGTC GGCCATCACT
    ATATTCCCTC AAAGGACTGA TGGCAAGCAT GACTTCCGAG TGTGGAACTC GCAGCTCATC
    CGCTATGCCG GCTACAAGCA GCCAGATGGC TCTACCTTGG GCGATCCAGC TAATGTGGAG
    TTCACAGAGA TCTGTATACA GCAGGGCTGG AAACCCCCAA GAGGCCGGTT CGATGTGCTG
    CCTCTCCTGC TCCAGGCCAA TGGCAATGAC CCTGAACTCT TTCAGATCCC CCCAGAGCTG
    GTGCTGGAAG TACCCATCAG GCACCCCAAG TTCGACTGGT TTAAGGACCT GGGGCTCAAA
    TGGTATGGCC TCCCCGCTGT GTCCAACATG CTGCTGGAGA TTGGGGGCCT GGAGTTCAGC
    GCCTGTCCCT TTAGTGGCTG GTACATGGGC ACGGAGATTG GCGTTCGTGA TTACTGTGAC
    AACTCTCGAT ACAACATCCT GGAGGAAGTA GCCAAGAAAA TGGATTTGGA CATGAGGAAG
    ACATCCTCCC TCTGGAAGGA CCAAGCCCTG GTGGAGATTA ACATTGCTGT CCTATACAGC
    TTCCAGAGTG ACAAGGTGAC CATTGTGGAC CACCACTCTG CCACCGAGTC CTTCATCAAA
    CACATGGAGA ATGAGTACCG CTGCAGAGGG GGCTGTCCCG CCGACTGGGT GTGGATTGTG
    CCCCCCATGT CGGGCAGCAT CACCCCTGTC TTCCACCAGG AGATGCTCAA CTACCGGCTC
    ACCCCGTCCT TTGAGTACCA GCCTGATCCA TGGAACACCC ACGTGTGGAA GGGCACCAAT
    GGGACCCCCA CGAAGCGGCG AGCCATTGGC TTCAAGAAAT TGGCAGAGGC CGTCAAGTTC
    TCAGCCAAGC TGATGGGACA GGCCATGGCC AAGAGAGTCA AAGCGACCAT TCTCTACGCC
    ACCGAGACAG GCAAATCCCA AGCCTATGCC AAGACCCTGT GCGAGATCTT CAAGCATGCC
    TTCGATGCCA AGGCTATGTC CATGGAGGAA TATGACATCG TGCACCTGGA GCACGAAGCC
    CTGGTCTTGG TGGTCACCAG CACCTTTGGC AATGGAGACC CCCCAGAGAA TGGGGAGAAA
    TTCGGCTGTG CTTTGATGGA GATGAGGCAC CCCAACTCTG TGCAAGAGGA GAGGAAGAGC
    TACAAGGTCC GATTCAACAG CGTCTCCTCC TATTCTGACT CCCGCAAGTC ATCAGGCGAT
    GGACCAGACC TCAGAGACAA CTTTGAAAGT ACTGGACCGC TGGCCAATGT GAGGTTCTCA
    GTCTTCGGCC TGGGCTCTCG GGCATACCCT CACTTCTGTG CCTTTGGGCA CGCAGTGGAC
    ACTCTCCTGG AGGAGCTGGG CGGGGAGAGG ATTCTGAAGA TGAGGGAGGG AGATGAGCTC
    TGCGGACAGG AAGAGGCTTT CAGGACCTGG GCTAAGAAAG TCTTCAAGGC AGCCTGTGAT
    GTGTTCTGTG TGGGGGATGA CGTCAACATT GAGAAGGCGA ACAATTCCCT CATTAGCAAT
    GACCGCAGCT GGAAGAGGAA TAAATTCCGC CTCACTTATG TGGCAGAAGC TCCAGAGCTG
    ACCCAAGGTC TTTCCAATGT TCACAAAAAG CGAGTCTCAG CCGCCCGACT CCTCAGCCGC
    CAAAACCTGC AAAGTCCTAA ATCCAGCCGA TCGACCATCT TCGTGCGTCT CCACACCAAC
    GGGAATCAGG AGTTGCAGTA CCAGCCAGGG GACCACCTGG GTGTCTTCCC TGGCAACCAT
    GAGGACCTCG TGAATGCACT CATTGAACGG CTGGAGGACG CGCCGCCTGC CAACCACGTG
    GTGAAGGTGG AAATGCTGGA GGAAAGGAAC ACGGCTCTGG GTGTCATCAG TAATTGGAAG
    GATGAATCTC GCCTCCCACC CTGCACCATC TTCCAGGCCT TCAAGTACTA CCTGGACATC
    ACCACGCCGC CCACGCCCCT GCAGCTGCAG CAGTTCGCCT CCCTGGCCAC CAATGAGAAA
    GAGAAGCAGC GGCTGCTGGT CCTCAGCAAG GGGCTTCAGG AATATGAGGA ATGGAAATGG
    GGCAAGAACC CCACAATGGT GGAGGTGCTG GAGGAGTTCC CGTCCATCCA GATGCCGGCC
    ACACTTCTCC TCACACAGCT GTCGCTGCTA CAACCTCGCT ACTATTCCAT CAGCTCCTCT
    CCAGACATGT ACCCCGACGA GGTGCACCTC ACCGTGGCCA TCGTGTCCTA CCATACCCGA
    GATGGAGAAG GACCGGTTCA CCACGGGGTG TGCTCCTCCT GGCTCAACCG AATACAGGCT
    GACGATGTCG TCCCCTGCTT CGTGAGAGGT GCCCCTAGCT TCCACCTGCC TCGAAACCCC
    CAGGTGCCTT GCATCCTGGT TGGCCCAGGC ACCGGCATCG CTCCCTTCCG AAGTTTTTGG
    CAACAGCGAC AATTTGACAT CCAACACAAA GGAATGAATC CGTGCCCCAT GGTTCTGGTC
    TTCGGGTGTC GACAATCCAA GATAGATCAT ATCTACAGAG AGGAGACCCT GCAGGCCAAG
    AACAAGGGCG TCTTCAGAGA GCTGTACACT GCCTACTCCC GGGAACCCGA CAGGCCAAAG
    AAATATGTAC AGGACGTGCT GCAGGAACAG CTGGCCGAGT CTGTGTACCG CGCCCTGAAG
    GAGCAAGGAG GCCATATTTA TGTCTGCGGG GACGTTACCA TGGCCGCTGA TGTCCTCAAA
    GCCATCCAGC GCATAATGAC CCAGCAGGGG AAACTCTCGG AGGAGGATGC TGGTGTGTTC
    ATCAGCAGGC TGAGGGATGA CAACCGGTAC CACGAGGACA TCTTTGGGGT CACCCTCAGA
    ACATATGAAG TGACCAACCG CCTTAGATCT GAGTCCATCG CCTTCATTGA AGAGAGCAAA
    AAAGACACAG ATGAGGTTTT CAGCTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu20278
    Clone ID Related Accession (Same CDS sequence) XM_017320707.1 , NM_008712.3
    Accession Version XM_017320707.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4290bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product nitric oxide synthase, brain isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAAGAGC ACACGTTTGG GGTCCAGCAG ATCCAACCCA ACGTCATTTC TGTCCGTCTC 
    TTCAAACGCA AAGTGGGAGG TCTGGGCTTC CTGGTGAAGG AACGGGTCAG CAAGCCCCCG
    GTGATCATCT CAGATCTGAT CCGAGGAGGT GCCGCGGAGC AGAGCGGCCT TATCCAAGCC
    GGCGACATCA TTCTCGCCGT CAATGATCGG CCCCTGGTAG ACCTCAGCTA TGACAGCGCT
    CTGGAAGTTC TCAGGGGCAT TGCCTCTGAG ACCCATGTGG TCCTCATTCT GAGAGGCCCT
    GAGGGCTTTA CTACACACCT GGAGACCACC TTCACAGGGG ATGGAACCCC CAAGACCATC
    CGTGTGACCC AGCCCCTGGG GACACCCACC AAAGCTGTCG ATCTGTCTCG CCAGCCATCA
    GCCAGCAAAG ACCAGCCATT AGCAGTAGAC AGGGTCCCAG GTCCCAGTAA CGGACCTCAG
    CATGCCCAAG GCCGTGGACA GGGAGCCGGC TCAGTCTCCC AGGCTAATGG TGTGGCCATT
    GACCCCACAA TGAAAAATAC CAAGGCCAAC CTCCAGGACA GCGGAGAACA GGATGAACTG
    CTCAAAGAGA TAGAACCTGT GTTGAGCATC CTCACCGGTG GGGGAAAAGC AGTCAACAGA
    GGGGGACCAG CCAAAGCAGA GATGAAAGAC ACAGGAATCC AGGTGGACAG AGACCTCGAC
    GGCAAACTGC ACAAAGCTCC GCCCCTGGGC GGGGAGAATG ATCGAGTCTT CAATGATCTG
    TGGGGGAAGG GCAACGTCCC TGTGGTCCTC AACAACCCGT ATTCAGAGAA CGAACAGTCT
    CCCGCCTCGG GCAAACAGTC TCCTACCAAG AATGGCAGTC CTTCCAGGTG CCCTCGCTTC
    CTTAAGGTCA AGAACTGGGA GACAGATGTG GTCCTCACTG ACACCCTGCA CCTGAAGAGC
    ACGCTGGAAA CGGGGTGCAC AGAGCAGATC TGCATGGGCT CCATCATGCT GCCATCCCAT
    CACATACGGA AGTCAGAAGA TGTCCGCACC AAGGACCAGC TCTTCCCTCT AGCCAAAGAA
    TTTCTCGACC AATACTACTC CTCCATTAAG AGGTTTGGCT CCAAGGCCCA CATGGACAGG
    CTGGAGGAGG TGAACAAGGA GATCGAGAGC ACCAGCACCT ACCAGCTCAA GGACACGGAG
    CTCATCTATG GCGCCAAGCA TGCCTGGCGG AACGCCTCTC GCTGTGTGGG CAGGATCCAG
    TGGTCCAAGC TGCAGGTGTT CGATGCCCGA GACTGCACCA CAGCCCATGG GATGTTCAAC
    TACATCTGTA ACCACGTCAA GTACGCCACC AACAAAGGGA ACCTCAGGTC GGCCATCACT
    ATATTCCCTC AAAGGACTGA TGGCAAGCAT GACTTCCGAG TGTGGAACTC GCAGCTCATC
    CGCTATGCCG GCTACAAGCA GCCAGATGGC TCTACCTTGG GCGATCCAGC TAATGTGGAG
    TTCACAGAGA TCTGTATACA GCAGGGCTGG AAACCCCCAA GAGGCCGGTT CGATGTGCTG
    CCTCTCCTGC TCCAGGCCAA TGGCAATGAC CCTGAACTCT TTCAGATCCC CCCAGAGCTG
    GTGCTGGAAG TACCCATCAG GCACCCCAAG TTCGACTGGT TTAAGGACCT GGGGCTCAAA
    TGGTATGGCC TCCCCGCTGT GTCCAACATG CTGCTGGAGA TTGGGGGCCT GGAGTTCAGC
    GCCTGTCCCT TTAGTGGCTG GTACATGGGC ACGGAGATTG GCGTTCGTGA TTACTGTGAC
    AACTCTCGAT ACAACATCCT GGAGGAAGTA GCCAAGAAAA TGGATTTGGA CATGAGGAAG
    ACATCCTCCC TCTGGAAGGA CCAAGCCCTG GTGGAGATTA ACATTGCTGT CCTATACAGC
    TTCCAGAGTG ACAAGGTGAC CATTGTGGAC CACCACTCTG CCACCGAGTC CTTCATCAAA
    CACATGGAGA ATGAGTACCG CTGCAGAGGG GGCTGTCCCG CCGACTGGGT GTGGATTGTG
    CCCCCCATGT CGGGCAGCAT CACCCCTGTC TTCCACCAGG AGATGCTCAA CTACCGGCTC
    ACCCCGTCCT TTGAGTACCA GCCTGATCCA TGGAACACCC ACGTGTGGAA GGGCACCAAT
    GGGACCCCCA CGAAGCGGCG AGCCATTGGC TTCAAGAAAT TGGCAGAGGC CGTCAAGTTC
    TCAGCCAAGC TGATGGGACA GGCCATGGCC AAGAGAGTCA AAGCGACCAT TCTCTACGCC
    ACCGAGACAG GCAAATCCCA AGCCTATGCC AAGACCCTGT GCGAGATCTT CAAGCATGCC
    TTCGATGCCA AGGCTATGTC CATGGAGGAA TATGACATCG TGCACCTGGA GCACGAAGCC
    CTGGTCTTGG TGGTCACCAG CACCTTTGGC AATGGAGACC CCCCAGAGAA TGGGGAGAAA
    TTCGGCTGTG CTTTGATGGA GATGAGGCAC CCCAACTCTG TGCAAGAGGA GAGGAAGAGC
    TACAAGGTCC GATTCAACAG CGTCTCCTCC TATTCTGACT CCCGCAAGTC ATCAGGCGAT
    GGACCAGACC TCAGAGACAA CTTTGAAAGT ACTGGACCGC TGGCCAATGT GAGGTTCTCA
    GTCTTCGGCC TGGGCTCTCG GGCATACCCT CACTTCTGTG CCTTTGGGCA CGCAGTGGAC
    ACTCTCCTGG AGGAGCTGGG CGGGGAGAGG ATTCTGAAGA TGAGGGAGGG AGATGAGCTC
    TGCGGACAGG AAGAGGCTTT CAGGACCTGG GCTAAGAAAG TCTTCAAGGC AGCCTGTGAT
    GTGTTCTGTG TGGGGGATGA CGTCAACATT GAGAAGGCGA ACAATTCCCT CATTAGCAAT
    GACCGCAGCT GGAAGAGGAA TAAATTCCGC CTCACTTATG TGGCAGAAGC TCCAGAGCTG
    ACCCAAGGTC TTTCCAATGT TCACAAAAAG CGAGTCTCAG CCGCCCGACT CCTCAGCCGC
    CAAAACCTGC AAAGTCCTAA ATCCAGCCGA TCGACCATCT TCGTGCGTCT CCACACCAAC
    GGGAATCAGG AGTTGCAGTA CCAGCCAGGG GACCACCTGG GTGTCTTCCC TGGCAACCAT
    GAGGACCTCG TGAATGCACT CATTGAACGG CTGGAGGACG CGCCGCCTGC CAACCACGTG
    GTGAAGGTGG AAATGCTGGA GGAAAGGAAC ACGGCTCTGG GTGTCATCAG TAATTGGAAG
    GATGAATCTC GCCTCCCACC CTGCACCATC TTCCAGGCCT TCAAGTACTA CCTGGACATC
    ACCACGCCGC CCACGCCCCT GCAGCTGCAG CAGTTCGCCT CCCTGGCCAC CAATGAGAAA
    GAGAAGCAGC GGCTGCTGGT CCTCAGCAAG GGGCTTCAGG AATATGAGGA ATGGAAATGG
    GGCAAGAACC CCACAATGGT GGAGGTGCTG GAGGAGTTCC CGTCCATCCA GATGCCGGCC
    ACACTTCTCC TCACACAGCT GTCGCTGCTA CAACCTCGCT ACTATTCCAT CAGCTCCTCT
    CCAGACATGT ACCCCGACGA GGTGCACCTC ACCGTGGCCA TCGTGTCCTA CCATACCCGA
    GATGGAGAAG GACCGGTTCA CCACGGGGTG TGCTCCTCCT GGCTCAACCG AATACAGGCT
    GACGATGTCG TCCCCTGCTT CGTGAGAGGT GCCCCTAGCT TCCACCTGCC TCGAAACCCC
    CAGGTGCCTT GCATCCTGGT TGGCCCAGGC ACCGGCATCG CTCCCTTCCG AAGTTTTTGG
    CAACAGCGAC AATTTGACAT CCAACACAAA GGAATGAATC CGTGCCCCAT GGTTCTGGTC
    TTCGGGTGTC GACAATCCAA GATAGATCAT ATCTACAGAG AGGAGACCCT GCAGGCCAAG
    AACAAGGGCG TCTTCAGAGA GCTGTACACT GCCTACTCCC GGGAACCCGA CAGGCCAAAG
    AAATATGTAC AGGACGTGCT GCAGGAACAG CTGGCCGAGT CTGTGTACCG CGCCCTGAAG
    GAGCAAGGAG GCCATATTTA TGTCTGCGGG GACGTTACCA TGGCCGCTGA TGTCCTCAAA
    GCCATCCAGC GCATAATGAC CCAGCAGGGG AAACTCTCGG AGGAGGATGC TGGTGTGTTC
    ATCAGCAGGC TGAGGGATGA CAACCGGTAC CACGAGGACA TCTTTGGGGT CACCCTCAGA
    ACATATGAAG TGACCAACCG CCTTAGATCT GAGTCCATCG CCTTCATTGA AGAGAGCAAA
    AAAGACACAG ATGAGGTTTT CAGCTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017176196.1
    CDS1109..5398
    Translation

    Target ORF information:

    RefSeq Version XM_017320707.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus nitric oxide synthase 1, neuronal (Nos1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017320707.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAAGAGC ACACGTTTGG GGTCCAGCAG ATCCAACCCA ACGTCATTTC TGTCCGTCTC 
    TTCAAACGCA AAGTGGGAGG TCTGGGCTTC CTGGTGAAGG AACGGGTCAG CAAGCCCCCG
    GTGATCATCT CAGATCTGAT CCGAGGAGGT GCCGCGGAGC AGAGCGGCCT TATCCAAGCC
    GGCGACATCA TTCTCGCCGT CAATGATCGG CCCCTGGTAG ACCTCAGCTA TGACAGCGCT
    CTGGAAGTTC TCAGGGGCAT TGCCTCTGAG ACCCATGTGG TCCTCATTCT GAGAGGCCCT
    GAGGGCTTTA CTACACACCT GGAGACCACC TTCACAGGGG ATGGAACCCC CAAGACCATC
    CGTGTGACCC AGCCCCTGGG GACACCCACC AAAGCTGTCG ATCTGTCTCG CCAGCCATCA
    GCCAGCAAAG ACCAGCCATT AGCAGTAGAC AGGGTCCCAG GTCCCAGTAA CGGACCTCAG
    CATGCCCAAG GCCGTGGACA GGGAGCCGGC TCAGTCTCCC AGGCTAATGG TGTGGCCATT
    GACCCCACAA TGAAAAATAC CAAGGCCAAC CTCCAGGACA GCGGAGAACA GGATGAACTG
    CTCAAAGAGA TAGAACCTGT GTTGAGCATC CTCACCGGTG GGGGAAAAGC AGTCAACAGA
    GGGGGACCAG CCAAAGCAGA GATGAAAGAC ACAGGAATCC AGGTGGACAG AGACCTCGAC
    GGCAAACTGC ACAAAGCTCC GCCCCTGGGC GGGGAGAATG ATCGAGTCTT CAATGATCTG
    TGGGGGAAGG GCAACGTCCC TGTGGTCCTC AACAACCCGT ATTCAGAGAA CGAACAGTCT
    CCCGCCTCGG GCAAACAGTC TCCTACCAAG AATGGCAGTC CTTCCAGGTG CCCTCGCTTC
    CTTAAGGTCA AGAACTGGGA GACAGATGTG GTCCTCACTG ACACCCTGCA CCTGAAGAGC
    ACGCTGGAAA CGGGGTGCAC AGAGCAGATC TGCATGGGCT CCATCATGCT GCCATCCCAT
    CACATACGGA AGTCAGAAGA TGTCCGCACC AAGGACCAGC TCTTCCCTCT AGCCAAAGAA
    TTTCTCGACC AATACTACTC CTCCATTAAG AGGTTTGGCT CCAAGGCCCA CATGGACAGG
    CTGGAGGAGG TGAACAAGGA GATCGAGAGC ACCAGCACCT ACCAGCTCAA GGACACGGAG
    CTCATCTATG GCGCCAAGCA TGCCTGGCGG AACGCCTCTC GCTGTGTGGG CAGGATCCAG
    TGGTCCAAGC TGCAGGTGTT CGATGCCCGA GACTGCACCA CAGCCCATGG GATGTTCAAC
    TACATCTGTA ACCACGTCAA GTACGCCACC AACAAAGGGA ACCTCAGGTC GGCCATCACT
    ATATTCCCTC AAAGGACTGA TGGCAAGCAT GACTTCCGAG TGTGGAACTC GCAGCTCATC
    CGCTATGCCG GCTACAAGCA GCCAGATGGC TCTACCTTGG GCGATCCAGC TAATGTGGAG
    TTCACAGAGA TCTGTATACA GCAGGGCTGG AAACCCCCAA GAGGCCGGTT CGATGTGCTG
    CCTCTCCTGC TCCAGGCCAA TGGCAATGAC CCTGAACTCT TTCAGATCCC CCCAGAGCTG
    GTGCTGGAAG TACCCATCAG GCACCCCAAG TTCGACTGGT TTAAGGACCT GGGGCTCAAA
    TGGTATGGCC TCCCCGCTGT GTCCAACATG CTGCTGGAGA TTGGGGGCCT GGAGTTCAGC
    GCCTGTCCCT TTAGTGGCTG GTACATGGGC ACGGAGATTG GCGTTCGTGA TTACTGTGAC
    AACTCTCGAT ACAACATCCT GGAGGAAGTA GCCAAGAAAA TGGATTTGGA CATGAGGAAG
    ACATCCTCCC TCTGGAAGGA CCAAGCCCTG GTGGAGATTA ACATTGCTGT CCTATACAGC
    TTCCAGAGTG ACAAGGTGAC CATTGTGGAC CACCACTCTG CCACCGAGTC CTTCATCAAA
    CACATGGAGA ATGAGTACCG CTGCAGAGGG GGCTGTCCCG CCGACTGGGT GTGGATTGTG
    CCCCCCATGT CGGGCAGCAT CACCCCTGTC TTCCACCAGG AGATGCTCAA CTACCGGCTC
    ACCCCGTCCT TTGAGTACCA GCCTGATCCA TGGAACACCC ACGTGTGGAA GGGCACCAAT
    GGGACCCCCA CGAAGCGGCG AGCCATTGGC TTCAAGAAAT TGGCAGAGGC CGTCAAGTTC
    TCAGCCAAGC TGATGGGACA GGCCATGGCC AAGAGAGTCA AAGCGACCAT TCTCTACGCC
    ACCGAGACAG GCAAATCCCA AGCCTATGCC AAGACCCTGT GCGAGATCTT CAAGCATGCC
    TTCGATGCCA AGGCTATGTC CATGGAGGAA TATGACATCG TGCACCTGGA GCACGAAGCC
    CTGGTCTTGG TGGTCACCAG CACCTTTGGC AATGGAGACC CCCCAGAGAA TGGGGAGAAA
    TTCGGCTGTG CTTTGATGGA GATGAGGCAC CCCAACTCTG TGCAAGAGGA GAGGAAGAGC
    TACAAGGTCC GATTCAACAG CGTCTCCTCC TATTCTGACT CCCGCAAGTC ATCAGGCGAT
    GGACCAGACC TCAGAGACAA CTTTGAAAGT ACTGGACCGC TGGCCAATGT GAGGTTCTCA
    GTCTTCGGCC TGGGCTCTCG GGCATACCCT CACTTCTGTG CCTTTGGGCA CGCAGTGGAC
    ACTCTCCTGG AGGAGCTGGG CGGGGAGAGG ATTCTGAAGA TGAGGGAGGG AGATGAGCTC
    TGCGGACAGG AAGAGGCTTT CAGGACCTGG GCTAAGAAAG TCTTCAAGGC AGCCTGTGAT
    GTGTTCTGTG TGGGGGATGA CGTCAACATT GAGAAGGCGA ACAATTCCCT CATTAGCAAT
    GACCGCAGCT GGAAGAGGAA TAAATTCCGC CTCACTTATG TGGCAGAAGC TCCAGAGCTG
    ACCCAAGGTC TTTCCAATGT TCACAAAAAG CGAGTCTCAG CCGCCCGACT CCTCAGCCGC
    CAAAACCTGC AAAGTCCTAA ATCCAGCCGA TCGACCATCT TCGTGCGTCT CCACACCAAC
    GGGAATCAGG AGTTGCAGTA CCAGCCAGGG GACCACCTGG GTGTCTTCCC TGGCAACCAT
    GAGGACCTCG TGAATGCACT CATTGAACGG CTGGAGGACG CGCCGCCTGC CAACCACGTG
    GTGAAGGTGG AAATGCTGGA GGAAAGGAAC ACGGCTCTGG GTGTCATCAG TAATTGGAAG
    GATGAATCTC GCCTCCCACC CTGCACCATC TTCCAGGCCT TCAAGTACTA CCTGGACATC
    ACCACGCCGC CCACGCCCCT GCAGCTGCAG CAGTTCGCCT CCCTGGCCAC CAATGAGAAA
    GAGAAGCAGC GGCTGCTGGT CCTCAGCAAG GGGCTTCAGG AATATGAGGA ATGGAAATGG
    GGCAAGAACC CCACAATGGT GGAGGTGCTG GAGGAGTTCC CGTCCATCCA GATGCCGGCC
    ACACTTCTCC TCACACAGCT GTCGCTGCTA CAACCTCGCT ACTATTCCAT CAGCTCCTCT
    CCAGACATGT ACCCCGACGA GGTGCACCTC ACCGTGGCCA TCGTGTCCTA CCATACCCGA
    GATGGAGAAG GACCGGTTCA CCACGGGGTG TGCTCCTCCT GGCTCAACCG AATACAGGCT
    GACGATGTCG TCCCCTGCTT CGTGAGAGGT GCCCCTAGCT TCCACCTGCC TCGAAACCCC
    CAGGTGCCTT GCATCCTGGT TGGCCCAGGC ACCGGCATCG CTCCCTTCCG AAGTTTTTGG
    CAACAGCGAC AATTTGACAT CCAACACAAA GGAATGAATC CGTGCCCCAT GGTTCTGGTC
    TTCGGGTGTC GACAATCCAA GATAGATCAT ATCTACAGAG AGGAGACCCT GCAGGCCAAG
    AACAAGGGCG TCTTCAGAGA GCTGTACACT GCCTACTCCC GGGAACCCGA CAGGCCAAAG
    AAATATGTAC AGGACGTGCT GCAGGAACAG CTGGCCGAGT CTGTGTACCG CGCCCTGAAG
    GAGCAAGGAG GCCATATTTA TGTCTGCGGG GACGTTACCA TGGCCGCTGA TGTCCTCAAA
    GCCATCCAGC GCATAATGAC CCAGCAGGGG AAACTCTCGG AGGAGGATGC TGGTGTGTTC
    ATCAGCAGGC TGAGGGATGA CAACCGGTAC CACGAGGACA TCTTTGGGGT CACCCTCAGA
    ACATATGAAG TGACCAACCG CCTTAGATCT GAGTCCATCG CCTTCATTGA AGAGAGCAAA
    AAAGACACAG ATGAGGTTTT CAGCTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu35811
    Clone ID Related Accession (Same CDS sequence) XM_006530189.3 , XM_006530188.3 , XM_006530187.3 , XM_006530190.1
    Accession Version XM_006530189.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4395bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product nitric oxide synthase, brain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006530189.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAAGAGC ACACGTTTGG GGTCCAGCAG ATCCAACCCA ACGTCATTTC TGTCCGTCTC 
    TTCAAACGCA AAGTGGGAGG TCTGGGCTTC CTGGTGAAGG AACGGGTCAG CAAGCCCCCG
    GTGATCATCT CAGATCTGAT CCGAGGAGGT GCCGCGGAGC AGAGCGGCCT TATCCAAGCC
    GGCGACATCA TTCTCGCCGT CAATGATCGG CCCCTGGTAG ACCTCAGCTA TGACAGCGCT
    CTGGAAGTTC TCAGGGGCAT TGCCTCTGAG ACCCATGTGG TCCTCATTCT GAGAGGCCCT
    GAGGGCTTTA CTACACACCT GGAGACCACC TTCACAGGGG ATGGAACCCC CAAGACCATC
    CGTGTGACCC AGCCCCTGGG GACACCCACC AAAGCTGTCG ATCTGTCTCG CCAGCCATCA
    GCCAGCAAAG ACCAGCCATT AGCAGTAGAC AGGGTCCCAG GTCCCAGTAA CGGACCTCAG
    CATGCCCAAG GCCGTGGACA GGGAGCCGGC TCAGTCTCCC AGGCTAATGG TGTGGCCATT
    GACCCCACAA TGAAAAATAC CAAGGCCAAC CTCCAGGACA GCGGAGAACA GGATGAACTG
    CTCAAAGAGA TAGAACCTGT GTTGAGCATC CTCACCGGTG GGGGAAAAGC AGTCAACAGA
    GGGGGACCAG CCAAAGCAGA GATGAAAGAC ACAGGAATCC AGGTGGACAG AGACCTCGAC
    GGCAAACTGC ACAAAGCTCC GCCCCTGGGC GGGGAGAATG ATCGAGTCTT CAATGATCTG
    TGGGGGAAGG GCAACGTCCC TGTGGTCCTC AACAACCCGT ATTCAGAGAA CGAACAGTCT
    CCCGCCTCGG GCAAACAGTC TCCTACCAAG AATGGCAGTC CTTCCAGGTG CCCTCGCTTC
    CTTAAGGTCA AGAACTGGGA GACAGATGTG GTCCTCACTG ACACCCTGCA CCTGAAGAGC
    ACGCTGGAAA CGGGGTGCAC AGAGCAGATC TGCATGGGCT CCATCATGCT GCCATCCCAT
    CACATACGGA AGTCAGAAGA TGTCCGCACC AAGGACCAGC TCTTCCCTCT AGCCAAAGAA
    TTTCTCGACC AATACTACTC CTCCATTAAG AGGTTTGGCT CCAAGGCCCA CATGGACAGG
    CTGGAGGAGG TGAACAAGGA GATCGAGAGC ACCAGCACCT ACCAGCTCAA GGACACGGAG
    CTCATCTATG GCGCCAAGCA TGCCTGGCGG AACGCCTCTC GCTGTGTGGG CAGGATCCAG
    TGGTCCAAGC TGCAGGTGTT CGATGCCCGA GACTGCACCA CAGCCCATGG GATGTTCAAC
    TACATCTGTA ACCACGTCAA GTACGCCACC AACAAAGGGA ACCTCAGGTC GGCCATCACT
    ATATTCCCTC AAAGGACTGA TGGCAAGCAT GACTTCCGAG TGTGGAACTC GCAGCTCATC
    CGCTATGCCG GCTACAAGCA GCCAGATGGC TCTACCTTGG GCGATCCAGC TAATGTGGAG
    TTCACAGAGA TCTGTATACA GCAGGGCTGG AAACCCCCAA GAGGCCGGTT CGATGTGCTG
    CCTCTCCTGC TCCAGGCCAA TGGCAATGAC CCTGAACTCT TTCAGATCCC CCCAGAGCTG
    GTGCTGGAAG TACCCATCAG GCACCCCAAG TTCGACTGGT TTAAGGACCT GGGGCTCAAA
    TGGTATGGCC TCCCCGCTGT GTCCAACATG CTGCTGGAGA TTGGGGGCCT GGAGTTCAGC
    GCCTGTCCCT TTAGTGGCTG GTACATGGGC ACGGAGATTG GCGTTCGTGA TTACTGTGAC
    AACTCTCGAT ACAACATCCT GGAGGAAGTA GCCAAGAAAA TGGATTTGGA CATGAGGAAG
    ACATCCTCCC TCTGGAAGGA CCAAGCCCTG GTGGAGATTA ACATTGCTGT CCTATACAGC
    TTCCAGAGTG ACAAGGTGAC CATTGTGGAC CACCACTCTG CCACCGAGTC CTTCATCAAA
    CACATGGAGA ATGAGTACCG CTGCAGAGGG GGCTGTCCCG CCGACTGGGT GTGGATTGTG
    CCCCCCATGT CGGGCAGCAT CACCCCTGTC TTCCACCAGG AGATGCTCAA CTACCGGCTC
    ACCCCGTCCT TTGAGTACCA GCCTGATCCA TGGAACACCC ACGTGTGGAA GGGCACCAAT
    GGGACCCCCA CGAAGCGGCG AGCCATTGGC TTCAAGAAAT TGGCAGAGGC CGTCAAGTTC
    TCAGCCAAGC TGATGGGACA GGCCATGGCC AAGAGAGTCA AAGCGACCAT TCTCTACGCC
    ACCGAGACAG GCAAATCCCA AGCCTATGCC AAGACCCTGT GCGAGATCTT CAAGCATGCC
    TTCGATGCCA AGGCTATGTC CATGGAGGAA TATGACATCG TGCACCTGGA GCACGAAGCC
    CTGGTCTTGG TGGTCACCAG CACCTTTGGC AATGGAGACC CCCCAGAGAA TGGGGAGAAA
    TTCGGCTGTG CTTTGATGGA GATGAGGCAC CCCAACTCTG TGCAAGAGGA GAGGAAGTAC
    CCGGAACCCT TGCGTTTCTT TCCCCGTAAA GGGCCTTCCC TCTCCCATGC TGACTCTGAA
    GCCCACAGTC TGGTTGCTGC CCGTGACAGC CAGCACAGGA GCTACAAGGT CCGATTCAAC
    AGCGTCTCCT CCTATTCTGA CTCCCGCAAG TCATCAGGCG ATGGACCAGA CCTCAGAGAC
    AACTTTGAAA GTACTGGACC GCTGGCCAAT GTGAGGTTCT CAGTCTTCGG CCTGGGCTCT
    CGGGCATACC CTCACTTCTG TGCCTTTGGG CACGCAGTGG ACACTCTCCT GGAGGAGCTG
    GGCGGGGAGA GGATTCTGAA GATGAGGGAG GGAGATGAGC TCTGCGGACA GGAAGAGGCT
    TTCAGGACCT GGGCTAAGAA AGTCTTCAAG GCAGCCTGTG ATGTGTTCTG TGTGGGGGAT
    GACGTCAACA TTGAGAAGGC GAACAATTCC CTCATTAGCA ATGACCGCAG CTGGAAGAGG
    AATAAATTCC GCCTCACTTA TGTGGCAGAA GCTCCAGAGC TGACCCAAGG TCTTTCCAAT
    GTTCACAAAA AGCGAGTCTC AGCCGCCCGA CTCCTCAGCC GCCAAAACCT GCAAAGTCCT
    AAATCCAGCC GATCGACCAT CTTCGTGCGT CTCCACACCA ACGGGAATCA GGAGTTGCAG
    TACCAGCCAG GGGACCACCT GGGTGTCTTC CCTGGCAACC ATGAGGACCT CGTGAATGCA
    CTCATTGAAC GGCTGGAGGA CGCGCCGCCT GCCAACCACG TGGTGAAGGT GGAAATGCTG
    GAGGAAAGGA ACACGGCTCT GGGTGTCATC AGTAATTGGA AGGATGAATC TCGCCTCCCA
    CCCTGCACCA TCTTCCAGGC CTTCAAGTAC TACCTGGACA TCACCACGCC GCCCACGCCC
    CTGCAGCTGC AGCAGTTCGC CTCCCTGGCC ACCAATGAGA AAGAGAAGCA GCGGCTGCTG
    GTCCTCAGCA AGGGGCTTCA GGAATATGAG GAATGGAAAT GGGGCAAGAA CCCCACAATG
    GTGGAGGTGC TGGAGGAGTT CCCGTCCATC CAGATGCCGG CCACACTTCT CCTCACACAG
    CTGTCGCTGC TACAACCTCG CTACTATTCC ATCAGCTCCT CTCCAGACAT GTACCCCGAC
    GAGGTGCACC TCACCGTGGC CATCGTGTCC TACCATACCC GAGATGGAGA AGGACCGGTT
    CACCACGGGG TGTGCTCCTC CTGGCTCAAC CGAATACAGG CTGACGATGT CGTCCCCTGC
    TTCGTGAGAG GTGCCCCTAG CTTCCACCTG CCTCGAAACC CCCAGGTGCC TTGCATCCTG
    GTTGGCCCAG GCACCGGCAT CGCTCCCTTC CGAAGTTTTT GGCAACAGCG ACAATTTGAC
    ATCCAACACA AAGGAATGAA TCCGTGCCCC ATGGTTCTGG TCTTCGGGTG TCGACAATCC
    AAGATAGATC ATATCTACAG AGAGGAGACC CTGCAGGCCA AGAACAAGGG CGTCTTCAGA
    GAGCTGTACA CTGCCTACTC CCGGGAACCC GACAGGCCAA AGAAATATGT ACAGGACGTG
    CTGCAGGAAC AGCTGGCCGA GTCTGTGTAC CGCGCCCTGA AGGAGCAAGG AGGCCATATT
    TATGTCTGCG GGGACGTTAC CATGGCCGCT GATGTCCTCA AAGCCATCCA GCGCATAATG
    ACCCAGCAGG GGAAACTCTC GGAGGAGGAT GCTGGTGTGT TCATCAGCAG GCTGAGGGAT
    GACAACCGGT ACCACGAGGA CATCTTTGGG GTCACCCTCA GAACATATGA AGTGACCAAC
    CGCCTTAGAT CTGAGTCCAT CGCCTTCATT GAAGAGAGCA AAAAAGACAC AGATGACAGG
    GTTTTCAGCT CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006530252.1
    CDS508..4902
    Translation

    Target ORF information:

    RefSeq Version XM_006530189.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus nitric oxide synthase 1, neuronal (Nos1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006530189.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAAGAGC ACACGTTTGG GGTCCAGCAG ATCCAACCCA ACGTCATTTC TGTCCGTCTC 
    TTCAAACGCA AAGTGGGAGG TCTGGGCTTC CTGGTGAAGG AACGGGTCAG CAAGCCCCCG
    GTGATCATCT CAGATCTGAT CCGAGGAGGT GCCGCGGAGC AGAGCGGCCT TATCCAAGCC
    GGCGACATCA TTCTCGCCGT CAATGATCGG CCCCTGGTAG ACCTCAGCTA TGACAGCGCT
    CTGGAAGTTC TCAGGGGCAT TGCCTCTGAG ACCCATGTGG TCCTCATTCT GAGAGGCCCT
    GAGGGCTTTA CTACACACCT GGAGACCACC TTCACAGGGG ATGGAACCCC CAAGACCATC
    CGTGTGACCC AGCCCCTGGG GACACCCACC AAAGCTGTCG ATCTGTCTCG CCAGCCATCA
    GCCAGCAAAG ACCAGCCATT AGCAGTAGAC AGGGTCCCAG GTCCCAGTAA CGGACCTCAG
    CATGCCCAAG GCCGTGGACA GGGAGCCGGC TCAGTCTCCC AGGCTAATGG TGTGGCCATT
    GACCCCACAA TGAAAAATAC CAAGGCCAAC CTCCAGGACA GCGGAGAACA GGATGAACTG
    CTCAAAGAGA TAGAACCTGT GTTGAGCATC CTCACCGGTG GGGGAAAAGC AGTCAACAGA
    GGGGGACCAG CCAAAGCAGA GATGAAAGAC ACAGGAATCC AGGTGGACAG AGACCTCGAC
    GGCAAACTGC ACAAAGCTCC GCCCCTGGGC GGGGAGAATG ATCGAGTCTT CAATGATCTG
    TGGGGGAAGG GCAACGTCCC TGTGGTCCTC AACAACCCGT ATTCAGAGAA CGAACAGTCT
    CCCGCCTCGG GCAAACAGTC TCCTACCAAG AATGGCAGTC CTTCCAGGTG CCCTCGCTTC
    CTTAAGGTCA AGAACTGGGA GACAGATGTG GTCCTCACTG ACACCCTGCA CCTGAAGAGC
    ACGCTGGAAA CGGGGTGCAC AGAGCAGATC TGCATGGGCT CCATCATGCT GCCATCCCAT
    CACATACGGA AGTCAGAAGA TGTCCGCACC AAGGACCAGC TCTTCCCTCT AGCCAAAGAA
    TTTCTCGACC AATACTACTC CTCCATTAAG AGGTTTGGCT CCAAGGCCCA CATGGACAGG
    CTGGAGGAGG TGAACAAGGA GATCGAGAGC ACCAGCACCT ACCAGCTCAA GGACACGGAG
    CTCATCTATG GCGCCAAGCA TGCCTGGCGG AACGCCTCTC GCTGTGTGGG CAGGATCCAG
    TGGTCCAAGC TGCAGGTGTT CGATGCCCGA GACTGCACCA CAGCCCATGG GATGTTCAAC
    TACATCTGTA ACCACGTCAA GTACGCCACC AACAAAGGGA ACCTCAGGTC GGCCATCACT
    ATATTCCCTC AAAGGACTGA TGGCAAGCAT GACTTCCGAG TGTGGAACTC GCAGCTCATC
    CGCTATGCCG GCTACAAGCA GCCAGATGGC TCTACCTTGG GCGATCCAGC TAATGTGGAG
    TTCACAGAGA TCTGTATACA GCAGGGCTGG AAACCCCCAA GAGGCCGGTT CGATGTGCTG
    CCTCTCCTGC TCCAGGCCAA TGGCAATGAC CCTGAACTCT TTCAGATCCC CCCAGAGCTG
    GTGCTGGAAG TACCCATCAG GCACCCCAAG TTCGACTGGT TTAAGGACCT GGGGCTCAAA
    TGGTATGGCC TCCCCGCTGT GTCCAACATG CTGCTGGAGA TTGGGGGCCT GGAGTTCAGC
    GCCTGTCCCT TTAGTGGCTG GTACATGGGC ACGGAGATTG GCGTTCGTGA TTACTGTGAC
    AACTCTCGAT ACAACATCCT GGAGGAAGTA GCCAAGAAAA TGGATTTGGA CATGAGGAAG
    ACATCCTCCC TCTGGAAGGA CCAAGCCCTG GTGGAGATTA ACATTGCTGT CCTATACAGC
    TTCCAGAGTG ACAAGGTGAC CATTGTGGAC CACCACTCTG CCACCGAGTC CTTCATCAAA
    CACATGGAGA ATGAGTACCG CTGCAGAGGG GGCTGTCCCG CCGACTGGGT GTGGATTGTG
    CCCCCCATGT CGGGCAGCAT CACCCCTGTC TTCCACCAGG AGATGCTCAA CTACCGGCTC
    ACCCCGTCCT TTGAGTACCA GCCTGATCCA TGGAACACCC ACGTGTGGAA GGGCACCAAT
    GGGACCCCCA CGAAGCGGCG AGCCATTGGC TTCAAGAAAT TGGCAGAGGC CGTCAAGTTC
    TCAGCCAAGC TGATGGGACA GGCCATGGCC AAGAGAGTCA AAGCGACCAT TCTCTACGCC
    ACCGAGACAG GCAAATCCCA AGCCTATGCC AAGACCCTGT GCGAGATCTT CAAGCATGCC
    TTCGATGCCA AGGCTATGTC CATGGAGGAA TATGACATCG TGCACCTGGA GCACGAAGCC
    CTGGTCTTGG TGGTCACCAG CACCTTTGGC AATGGAGACC CCCCAGAGAA TGGGGAGAAA
    TTCGGCTGTG CTTTGATGGA GATGAGGCAC CCCAACTCTG TGCAAGAGGA GAGGAAGTAC
    CCGGAACCCT TGCGTTTCTT TCCCCGTAAA GGGCCTTCCC TCTCCCATGC TGACTCTGAA
    GCCCACAGTC TGGTTGCTGC CCGTGACAGC CAGCACAGGA GCTACAAGGT CCGATTCAAC
    AGCGTCTCCT CCTATTCTGA CTCCCGCAAG TCATCAGGCG ATGGACCAGA CCTCAGAGAC
    AACTTTGAAA GTACTGGACC GCTGGCCAAT GTGAGGTTCT CAGTCTTCGG CCTGGGCTCT
    CGGGCATACC CTCACTTCTG TGCCTTTGGG CACGCAGTGG ACACTCTCCT GGAGGAGCTG
    GGCGGGGAGA GGATTCTGAA GATGAGGGAG GGAGATGAGC TCTGCGGACA GGAAGAGGCT
    TTCAGGACCT GGGCTAAGAA AGTCTTCAAG GCAGCCTGTG ATGTGTTCTG TGTGGGGGAT
    GACGTCAACA TTGAGAAGGC GAACAATTCC CTCATTAGCA ATGACCGCAG CTGGAAGAGG
    AATAAATTCC GCCTCACTTA TGTGGCAGAA GCTCCAGAGC TGACCCAAGG TCTTTCCAAT
    GTTCACAAAA AGCGAGTCTC AGCCGCCCGA CTCCTCAGCC GCCAAAACCT GCAAAGTCCT
    AAATCCAGCC GATCGACCAT CTTCGTGCGT CTCCACACCA ACGGGAATCA GGAGTTGCAG
    TACCAGCCAG GGGACCACCT GGGTGTCTTC CCTGGCAACC ATGAGGACCT CGTGAATGCA
    CTCATTGAAC GGCTGGAGGA CGCGCCGCCT GCCAACCACG TGGTGAAGGT GGAAATGCTG
    GAGGAAAGGA ACACGGCTCT GGGTGTCATC AGTAATTGGA AGGATGAATC TCGCCTCCCA
    CCCTGCACCA TCTTCCAGGC CTTCAAGTAC TACCTGGACA TCACCACGCC GCCCACGCCC
    CTGCAGCTGC AGCAGTTCGC CTCCCTGGCC ACCAATGAGA AAGAGAAGCA GCGGCTGCTG
    GTCCTCAGCA AGGGGCTTCA GGAATATGAG GAATGGAAAT GGGGCAAGAA CCCCACAATG
    GTGGAGGTGC TGGAGGAGTT CCCGTCCATC CAGATGCCGG CCACACTTCT CCTCACACAG
    CTGTCGCTGC TACAACCTCG CTACTATTCC ATCAGCTCCT CTCCAGACAT GTACCCCGAC
    GAGGTGCACC TCACCGTGGC CATCGTGTCC TACCATACCC GAGATGGAGA AGGACCGGTT
    CACCACGGGG TGTGCTCCTC CTGGCTCAAC CGAATACAGG CTGACGATGT CGTCCCCTGC
    TTCGTGAGAG GTGCCCCTAG CTTCCACCTG CCTCGAAACC CCCAGGTGCC TTGCATCCTG
    GTTGGCCCAG GCACCGGCAT CGCTCCCTTC CGAAGTTTTT GGCAACAGCG ACAATTTGAC
    ATCCAACACA AAGGAATGAA TCCGTGCCCC ATGGTTCTGG TCTTCGGGTG TCGACAATCC
    AAGATAGATC ATATCTACAG AGAGGAGACC CTGCAGGCCA AGAACAAGGG CGTCTTCAGA
    GAGCTGTACA CTGCCTACTC CCGGGAACCC GACAGGCCAA AGAAATATGT ACAGGACGTG
    CTGCAGGAAC AGCTGGCCGA GTCTGTGTAC CGCGCCCTGA AGGAGCAAGG AGGCCATATT
    TATGTCTGCG GGGACGTTAC CATGGCCGCT GATGTCCTCA AAGCCATCCA GCGCATAATG
    ACCCAGCAGG GGAAACTCTC GGAGGAGGAT GCTGGTGTGT TCATCAGCAG GCTGAGGGAT
    GACAACCGGT ACCACGAGGA CATCTTTGGG GTCACCCTCA GAACATATGA AGTGACCAAC
    CGCCTTAGAT CTGAGTCCAT CGCCTTCATT GAAGAGAGCA AAAAAGACAC AGATGACAGG
    GTTTTCAGCT CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu35811
    Clone ID Related Accession (Same CDS sequence) XM_006530189.3 , XM_006530188.3 , XM_006530187.3 , XM_006530190.1
    Accession Version XM_006530188.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4395bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product nitric oxide synthase, brain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006530188.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAAGAGC ACACGTTTGG GGTCCAGCAG ATCCAACCCA ACGTCATTTC TGTCCGTCTC 
    TTCAAACGCA AAGTGGGAGG TCTGGGCTTC CTGGTGAAGG AACGGGTCAG CAAGCCCCCG
    GTGATCATCT CAGATCTGAT CCGAGGAGGT GCCGCGGAGC AGAGCGGCCT TATCCAAGCC
    GGCGACATCA TTCTCGCCGT CAATGATCGG CCCCTGGTAG ACCTCAGCTA TGACAGCGCT
    CTGGAAGTTC TCAGGGGCAT TGCCTCTGAG ACCCATGTGG TCCTCATTCT GAGAGGCCCT
    GAGGGCTTTA CTACACACCT GGAGACCACC TTCACAGGGG ATGGAACCCC CAAGACCATC
    CGTGTGACCC AGCCCCTGGG GACACCCACC AAAGCTGTCG ATCTGTCTCG CCAGCCATCA
    GCCAGCAAAG ACCAGCCATT AGCAGTAGAC AGGGTCCCAG GTCCCAGTAA CGGACCTCAG
    CATGCCCAAG GCCGTGGACA GGGAGCCGGC TCAGTCTCCC AGGCTAATGG TGTGGCCATT
    GACCCCACAA TGAAAAATAC CAAGGCCAAC CTCCAGGACA GCGGAGAACA GGATGAACTG
    CTCAAAGAGA TAGAACCTGT GTTGAGCATC CTCACCGGTG GGGGAAAAGC AGTCAACAGA
    GGGGGACCAG CCAAAGCAGA GATGAAAGAC ACAGGAATCC AGGTGGACAG AGACCTCGAC
    GGCAAACTGC ACAAAGCTCC GCCCCTGGGC GGGGAGAATG ATCGAGTCTT CAATGATCTG
    TGGGGGAAGG GCAACGTCCC TGTGGTCCTC AACAACCCGT ATTCAGAGAA CGAACAGTCT
    CCCGCCTCGG GCAAACAGTC TCCTACCAAG AATGGCAGTC CTTCCAGGTG CCCTCGCTTC
    CTTAAGGTCA AGAACTGGGA GACAGATGTG GTCCTCACTG ACACCCTGCA CCTGAAGAGC
    ACGCTGGAAA CGGGGTGCAC AGAGCAGATC TGCATGGGCT CCATCATGCT GCCATCCCAT
    CACATACGGA AGTCAGAAGA TGTCCGCACC AAGGACCAGC TCTTCCCTCT AGCCAAAGAA
    TTTCTCGACC AATACTACTC CTCCATTAAG AGGTTTGGCT CCAAGGCCCA CATGGACAGG
    CTGGAGGAGG TGAACAAGGA GATCGAGAGC ACCAGCACCT ACCAGCTCAA GGACACGGAG
    CTCATCTATG GCGCCAAGCA TGCCTGGCGG AACGCCTCTC GCTGTGTGGG CAGGATCCAG
    TGGTCCAAGC TGCAGGTGTT CGATGCCCGA GACTGCACCA CAGCCCATGG GATGTTCAAC
    TACATCTGTA ACCACGTCAA GTACGCCACC AACAAAGGGA ACCTCAGGTC GGCCATCACT
    ATATTCCCTC AAAGGACTGA TGGCAAGCAT GACTTCCGAG TGTGGAACTC GCAGCTCATC
    CGCTATGCCG GCTACAAGCA GCCAGATGGC TCTACCTTGG GCGATCCAGC TAATGTGGAG
    TTCACAGAGA TCTGTATACA GCAGGGCTGG AAACCCCCAA GAGGCCGGTT CGATGTGCTG
    CCTCTCCTGC TCCAGGCCAA TGGCAATGAC CCTGAACTCT TTCAGATCCC CCCAGAGCTG
    GTGCTGGAAG TACCCATCAG GCACCCCAAG TTCGACTGGT TTAAGGACCT GGGGCTCAAA
    TGGTATGGCC TCCCCGCTGT GTCCAACATG CTGCTGGAGA TTGGGGGCCT GGAGTTCAGC
    GCCTGTCCCT TTAGTGGCTG GTACATGGGC ACGGAGATTG GCGTTCGTGA TTACTGTGAC
    AACTCTCGAT ACAACATCCT GGAGGAAGTA GCCAAGAAAA TGGATTTGGA CATGAGGAAG
    ACATCCTCCC TCTGGAAGGA CCAAGCCCTG GTGGAGATTA ACATTGCTGT CCTATACAGC
    TTCCAGAGTG ACAAGGTGAC CATTGTGGAC CACCACTCTG CCACCGAGTC CTTCATCAAA
    CACATGGAGA ATGAGTACCG CTGCAGAGGG GGCTGTCCCG CCGACTGGGT GTGGATTGTG
    CCCCCCATGT CGGGCAGCAT CACCCCTGTC TTCCACCAGG AGATGCTCAA CTACCGGCTC
    ACCCCGTCCT TTGAGTACCA GCCTGATCCA TGGAACACCC ACGTGTGGAA GGGCACCAAT
    GGGACCCCCA CGAAGCGGCG AGCCATTGGC TTCAAGAAAT TGGCAGAGGC CGTCAAGTTC
    TCAGCCAAGC TGATGGGACA GGCCATGGCC AAGAGAGTCA AAGCGACCAT TCTCTACGCC
    ACCGAGACAG GCAAATCCCA AGCCTATGCC AAGACCCTGT GCGAGATCTT CAAGCATGCC
    TTCGATGCCA AGGCTATGTC CATGGAGGAA TATGACATCG TGCACCTGGA GCACGAAGCC
    CTGGTCTTGG TGGTCACCAG CACCTTTGGC AATGGAGACC CCCCAGAGAA TGGGGAGAAA
    TTCGGCTGTG CTTTGATGGA GATGAGGCAC CCCAACTCTG TGCAAGAGGA GAGGAAGTAC
    CCGGAACCCT TGCGTTTCTT TCCCCGTAAA GGGCCTTCCC TCTCCCATGC TGACTCTGAA
    GCCCACAGTC TGGTTGCTGC CCGTGACAGC CAGCACAGGA GCTACAAGGT CCGATTCAAC
    AGCGTCTCCT CCTATTCTGA CTCCCGCAAG TCATCAGGCG ATGGACCAGA CCTCAGAGAC
    AACTTTGAAA GTACTGGACC GCTGGCCAAT GTGAGGTTCT CAGTCTTCGG CCTGGGCTCT
    CGGGCATACC CTCACTTCTG TGCCTTTGGG CACGCAGTGG ACACTCTCCT GGAGGAGCTG
    GGCGGGGAGA GGATTCTGAA GATGAGGGAG GGAGATGAGC TCTGCGGACA GGAAGAGGCT
    TTCAGGACCT GGGCTAAGAA AGTCTTCAAG GCAGCCTGTG ATGTGTTCTG TGTGGGGGAT
    GACGTCAACA TTGAGAAGGC GAACAATTCC CTCATTAGCA ATGACCGCAG CTGGAAGAGG
    AATAAATTCC GCCTCACTTA TGTGGCAGAA GCTCCAGAGC TGACCCAAGG TCTTTCCAAT
    GTTCACAAAA AGCGAGTCTC AGCCGCCCGA CTCCTCAGCC GCCAAAACCT GCAAAGTCCT
    AAATCCAGCC GATCGACCAT CTTCGTGCGT CTCCACACCA ACGGGAATCA GGAGTTGCAG
    TACCAGCCAG GGGACCACCT GGGTGTCTTC CCTGGCAACC ATGAGGACCT CGTGAATGCA
    CTCATTGAAC GGCTGGAGGA CGCGCCGCCT GCCAACCACG TGGTGAAGGT GGAAATGCTG
    GAGGAAAGGA ACACGGCTCT GGGTGTCATC AGTAATTGGA AGGATGAATC TCGCCTCCCA
    CCCTGCACCA TCTTCCAGGC CTTCAAGTAC TACCTGGACA TCACCACGCC GCCCACGCCC
    CTGCAGCTGC AGCAGTTCGC CTCCCTGGCC ACCAATGAGA AAGAGAAGCA GCGGCTGCTG
    GTCCTCAGCA AGGGGCTTCA GGAATATGAG GAATGGAAAT GGGGCAAGAA CCCCACAATG
    GTGGAGGTGC TGGAGGAGTT CCCGTCCATC CAGATGCCGG CCACACTTCT CCTCACACAG
    CTGTCGCTGC TACAACCTCG CTACTATTCC ATCAGCTCCT CTCCAGACAT GTACCCCGAC
    GAGGTGCACC TCACCGTGGC CATCGTGTCC TACCATACCC GAGATGGAGA AGGACCGGTT
    CACCACGGGG TGTGCTCCTC CTGGCTCAAC CGAATACAGG CTGACGATGT CGTCCCCTGC
    TTCGTGAGAG GTGCCCCTAG CTTCCACCTG CCTCGAAACC CCCAGGTGCC TTGCATCCTG
    GTTGGCCCAG GCACCGGCAT CGCTCCCTTC CGAAGTTTTT GGCAACAGCG ACAATTTGAC
    ATCCAACACA AAGGAATGAA TCCGTGCCCC ATGGTTCTGG TCTTCGGGTG TCGACAATCC
    AAGATAGATC ATATCTACAG AGAGGAGACC CTGCAGGCCA AGAACAAGGG CGTCTTCAGA
    GAGCTGTACA CTGCCTACTC CCGGGAACCC GACAGGCCAA AGAAATATGT ACAGGACGTG
    CTGCAGGAAC AGCTGGCCGA GTCTGTGTAC CGCGCCCTGA AGGAGCAAGG AGGCCATATT
    TATGTCTGCG GGGACGTTAC CATGGCCGCT GATGTCCTCA AAGCCATCCA GCGCATAATG
    ACCCAGCAGG GGAAACTCTC GGAGGAGGAT GCTGGTGTGT TCATCAGCAG GCTGAGGGAT
    GACAACCGGT ACCACGAGGA CATCTTTGGG GTCACCCTCA GAACATATGA AGTGACCAAC
    CGCCTTAGAT CTGAGTCCAT CGCCTTCATT GAAGAGAGCA AAAAAGACAC AGATGACAGG
    GTTTTCAGCT CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006530251.1
    CDS541..4935
    Translation

    Target ORF information:

    RefSeq Version XM_006530188.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus nitric oxide synthase 1, neuronal (Nos1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006530188.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAAGAGC ACACGTTTGG GGTCCAGCAG ATCCAACCCA ACGTCATTTC TGTCCGTCTC 
    TTCAAACGCA AAGTGGGAGG TCTGGGCTTC CTGGTGAAGG AACGGGTCAG CAAGCCCCCG
    GTGATCATCT CAGATCTGAT CCGAGGAGGT GCCGCGGAGC AGAGCGGCCT TATCCAAGCC
    GGCGACATCA TTCTCGCCGT CAATGATCGG CCCCTGGTAG ACCTCAGCTA TGACAGCGCT
    CTGGAAGTTC TCAGGGGCAT TGCCTCTGAG ACCCATGTGG TCCTCATTCT GAGAGGCCCT
    GAGGGCTTTA CTACACACCT GGAGACCACC TTCACAGGGG ATGGAACCCC CAAGACCATC
    CGTGTGACCC AGCCCCTGGG GACACCCACC AAAGCTGTCG ATCTGTCTCG CCAGCCATCA
    GCCAGCAAAG ACCAGCCATT AGCAGTAGAC AGGGTCCCAG GTCCCAGTAA CGGACCTCAG
    CATGCCCAAG GCCGTGGACA GGGAGCCGGC TCAGTCTCCC AGGCTAATGG TGTGGCCATT
    GACCCCACAA TGAAAAATAC CAAGGCCAAC CTCCAGGACA GCGGAGAACA GGATGAACTG
    CTCAAAGAGA TAGAACCTGT GTTGAGCATC CTCACCGGTG GGGGAAAAGC AGTCAACAGA
    GGGGGACCAG CCAAAGCAGA GATGAAAGAC ACAGGAATCC AGGTGGACAG AGACCTCGAC
    GGCAAACTGC ACAAAGCTCC GCCCCTGGGC GGGGAGAATG ATCGAGTCTT CAATGATCTG
    TGGGGGAAGG GCAACGTCCC TGTGGTCCTC AACAACCCGT ATTCAGAGAA CGAACAGTCT
    CCCGCCTCGG GCAAACAGTC TCCTACCAAG AATGGCAGTC CTTCCAGGTG CCCTCGCTTC
    CTTAAGGTCA AGAACTGGGA GACAGATGTG GTCCTCACTG ACACCCTGCA CCTGAAGAGC
    ACGCTGGAAA CGGGGTGCAC AGAGCAGATC TGCATGGGCT CCATCATGCT GCCATCCCAT
    CACATACGGA AGTCAGAAGA TGTCCGCACC AAGGACCAGC TCTTCCCTCT AGCCAAAGAA
    TTTCTCGACC AATACTACTC CTCCATTAAG AGGTTTGGCT CCAAGGCCCA CATGGACAGG
    CTGGAGGAGG TGAACAAGGA GATCGAGAGC ACCAGCACCT ACCAGCTCAA GGACACGGAG
    CTCATCTATG GCGCCAAGCA TGCCTGGCGG AACGCCTCTC GCTGTGTGGG CAGGATCCAG
    TGGTCCAAGC TGCAGGTGTT CGATGCCCGA GACTGCACCA CAGCCCATGG GATGTTCAAC
    TACATCTGTA ACCACGTCAA GTACGCCACC AACAAAGGGA ACCTCAGGTC GGCCATCACT
    ATATTCCCTC AAAGGACTGA TGGCAAGCAT GACTTCCGAG TGTGGAACTC GCAGCTCATC
    CGCTATGCCG GCTACAAGCA GCCAGATGGC TCTACCTTGG GCGATCCAGC TAATGTGGAG
    TTCACAGAGA TCTGTATACA GCAGGGCTGG AAACCCCCAA GAGGCCGGTT CGATGTGCTG
    CCTCTCCTGC TCCAGGCCAA TGGCAATGAC CCTGAACTCT TTCAGATCCC CCCAGAGCTG
    GTGCTGGAAG TACCCATCAG GCACCCCAAG TTCGACTGGT TTAAGGACCT GGGGCTCAAA
    TGGTATGGCC TCCCCGCTGT GTCCAACATG CTGCTGGAGA TTGGGGGCCT GGAGTTCAGC
    GCCTGTCCCT TTAGTGGCTG GTACATGGGC ACGGAGATTG GCGTTCGTGA TTACTGTGAC
    AACTCTCGAT ACAACATCCT GGAGGAAGTA GCCAAGAAAA TGGATTTGGA CATGAGGAAG
    ACATCCTCCC TCTGGAAGGA CCAAGCCCTG GTGGAGATTA ACATTGCTGT CCTATACAGC
    TTCCAGAGTG ACAAGGTGAC CATTGTGGAC CACCACTCTG CCACCGAGTC CTTCATCAAA
    CACATGGAGA ATGAGTACCG CTGCAGAGGG GGCTGTCCCG CCGACTGGGT GTGGATTGTG
    CCCCCCATGT CGGGCAGCAT CACCCCTGTC TTCCACCAGG AGATGCTCAA CTACCGGCTC
    ACCCCGTCCT TTGAGTACCA GCCTGATCCA TGGAACACCC ACGTGTGGAA GGGCACCAAT
    GGGACCCCCA CGAAGCGGCG AGCCATTGGC TTCAAGAAAT TGGCAGAGGC CGTCAAGTTC
    TCAGCCAAGC TGATGGGACA GGCCATGGCC AAGAGAGTCA AAGCGACCAT TCTCTACGCC
    ACCGAGACAG GCAAATCCCA AGCCTATGCC AAGACCCTGT GCGAGATCTT CAAGCATGCC
    TTCGATGCCA AGGCTATGTC CATGGAGGAA TATGACATCG TGCACCTGGA GCACGAAGCC
    CTGGTCTTGG TGGTCACCAG CACCTTTGGC AATGGAGACC CCCCAGAGAA TGGGGAGAAA
    TTCGGCTGTG CTTTGATGGA GATGAGGCAC CCCAACTCTG TGCAAGAGGA GAGGAAGTAC
    CCGGAACCCT TGCGTTTCTT TCCCCGTAAA GGGCCTTCCC TCTCCCATGC TGACTCTGAA
    GCCCACAGTC TGGTTGCTGC CCGTGACAGC CAGCACAGGA GCTACAAGGT CCGATTCAAC
    AGCGTCTCCT CCTATTCTGA CTCCCGCAAG TCATCAGGCG ATGGACCAGA CCTCAGAGAC
    AACTTTGAAA GTACTGGACC GCTGGCCAAT GTGAGGTTCT CAGTCTTCGG CCTGGGCTCT
    CGGGCATACC CTCACTTCTG TGCCTTTGGG CACGCAGTGG ACACTCTCCT GGAGGAGCTG
    GGCGGGGAGA GGATTCTGAA GATGAGGGAG GGAGATGAGC TCTGCGGACA GGAAGAGGCT
    TTCAGGACCT GGGCTAAGAA AGTCTTCAAG GCAGCCTGTG ATGTGTTCTG TGTGGGGGAT
    GACGTCAACA TTGAGAAGGC GAACAATTCC CTCATTAGCA ATGACCGCAG CTGGAAGAGG
    AATAAATTCC GCCTCACTTA TGTGGCAGAA GCTCCAGAGC TGACCCAAGG TCTTTCCAAT
    GTTCACAAAA AGCGAGTCTC AGCCGCCCGA CTCCTCAGCC GCCAAAACCT GCAAAGTCCT
    AAATCCAGCC GATCGACCAT CTTCGTGCGT CTCCACACCA ACGGGAATCA GGAGTTGCAG
    TACCAGCCAG GGGACCACCT GGGTGTCTTC CCTGGCAACC ATGAGGACCT CGTGAATGCA
    CTCATTGAAC GGCTGGAGGA CGCGCCGCCT GCCAACCACG TGGTGAAGGT GGAAATGCTG
    GAGGAAAGGA ACACGGCTCT GGGTGTCATC AGTAATTGGA AGGATGAATC TCGCCTCCCA
    CCCTGCACCA TCTTCCAGGC CTTCAAGTAC TACCTGGACA TCACCACGCC GCCCACGCCC
    CTGCAGCTGC AGCAGTTCGC CTCCCTGGCC ACCAATGAGA AAGAGAAGCA GCGGCTGCTG
    GTCCTCAGCA AGGGGCTTCA GGAATATGAG GAATGGAAAT GGGGCAAGAA CCCCACAATG
    GTGGAGGTGC TGGAGGAGTT CCCGTCCATC CAGATGCCGG CCACACTTCT CCTCACACAG
    CTGTCGCTGC TACAACCTCG CTACTATTCC ATCAGCTCCT CTCCAGACAT GTACCCCGAC
    GAGGTGCACC TCACCGTGGC CATCGTGTCC TACCATACCC GAGATGGAGA AGGACCGGTT
    CACCACGGGG TGTGCTCCTC CTGGCTCAAC CGAATACAGG CTGACGATGT CGTCCCCTGC
    TTCGTGAGAG GTGCCCCTAG CTTCCACCTG CCTCGAAACC CCCAGGTGCC TTGCATCCTG
    GTTGGCCCAG GCACCGGCAT CGCTCCCTTC CGAAGTTTTT GGCAACAGCG ACAATTTGAC
    ATCCAACACA AAGGAATGAA TCCGTGCCCC ATGGTTCTGG TCTTCGGGTG TCGACAATCC
    AAGATAGATC ATATCTACAG AGAGGAGACC CTGCAGGCCA AGAACAAGGG CGTCTTCAGA
    GAGCTGTACA CTGCCTACTC CCGGGAACCC GACAGGCCAA AGAAATATGT ACAGGACGTG
    CTGCAGGAAC AGCTGGCCGA GTCTGTGTAC CGCGCCCTGA AGGAGCAAGG AGGCCATATT
    TATGTCTGCG GGGACGTTAC CATGGCCGCT GATGTCCTCA AAGCCATCCA GCGCATAATG
    ACCCAGCAGG GGAAACTCTC GGAGGAGGAT GCTGGTGTGT TCATCAGCAG GCTGAGGGAT
    GACAACCGGT ACCACGAGGA CATCTTTGGG GTCACCCTCA GAACATATGA AGTGACCAAC
    CGCCTTAGAT CTGAGTCCAT CGCCTTCATT GAAGAGAGCA AAAAAGACAC AGATGACAGG
    GTTTTCAGCT CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu35811
    Clone ID Related Accession (Same CDS sequence) XM_006530189.3 , XM_006530188.3 , XM_006530187.3 , XM_006530190.1
    Accession Version XM_006530187.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4395bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product nitric oxide synthase, brain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006530187.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAAGAGC ACACGTTTGG GGTCCAGCAG ATCCAACCCA ACGTCATTTC TGTCCGTCTC 
    TTCAAACGCA AAGTGGGAGG TCTGGGCTTC CTGGTGAAGG AACGGGTCAG CAAGCCCCCG
    GTGATCATCT CAGATCTGAT CCGAGGAGGT GCCGCGGAGC AGAGCGGCCT TATCCAAGCC
    GGCGACATCA TTCTCGCCGT CAATGATCGG CCCCTGGTAG ACCTCAGCTA TGACAGCGCT
    CTGGAAGTTC TCAGGGGCAT TGCCTCTGAG ACCCATGTGG TCCTCATTCT GAGAGGCCCT
    GAGGGCTTTA CTACACACCT GGAGACCACC TTCACAGGGG ATGGAACCCC CAAGACCATC
    CGTGTGACCC AGCCCCTGGG GACACCCACC AAAGCTGTCG ATCTGTCTCG CCAGCCATCA
    GCCAGCAAAG ACCAGCCATT AGCAGTAGAC AGGGTCCCAG GTCCCAGTAA CGGACCTCAG
    CATGCCCAAG GCCGTGGACA GGGAGCCGGC TCAGTCTCCC AGGCTAATGG TGTGGCCATT
    GACCCCACAA TGAAAAATAC CAAGGCCAAC CTCCAGGACA GCGGAGAACA GGATGAACTG
    CTCAAAGAGA TAGAACCTGT GTTGAGCATC CTCACCGGTG GGGGAAAAGC AGTCAACAGA
    GGGGGACCAG CCAAAGCAGA GATGAAAGAC ACAGGAATCC AGGTGGACAG AGACCTCGAC
    GGCAAACTGC ACAAAGCTCC GCCCCTGGGC GGGGAGAATG ATCGAGTCTT CAATGATCTG
    TGGGGGAAGG GCAACGTCCC TGTGGTCCTC AACAACCCGT ATTCAGAGAA CGAACAGTCT
    CCCGCCTCGG GCAAACAGTC TCCTACCAAG AATGGCAGTC CTTCCAGGTG CCCTCGCTTC
    CTTAAGGTCA AGAACTGGGA GACAGATGTG GTCCTCACTG ACACCCTGCA CCTGAAGAGC
    ACGCTGGAAA CGGGGTGCAC AGAGCAGATC TGCATGGGCT CCATCATGCT GCCATCCCAT
    CACATACGGA AGTCAGAAGA TGTCCGCACC AAGGACCAGC TCTTCCCTCT AGCCAAAGAA
    TTTCTCGACC AATACTACTC CTCCATTAAG AGGTTTGGCT CCAAGGCCCA CATGGACAGG
    CTGGAGGAGG TGAACAAGGA GATCGAGAGC ACCAGCACCT ACCAGCTCAA GGACACGGAG
    CTCATCTATG GCGCCAAGCA TGCCTGGCGG AACGCCTCTC GCTGTGTGGG CAGGATCCAG
    TGGTCCAAGC TGCAGGTGTT CGATGCCCGA GACTGCACCA CAGCCCATGG GATGTTCAAC
    TACATCTGTA ACCACGTCAA GTACGCCACC AACAAAGGGA ACCTCAGGTC GGCCATCACT
    ATATTCCCTC AAAGGACTGA TGGCAAGCAT GACTTCCGAG TGTGGAACTC GCAGCTCATC
    CGCTATGCCG GCTACAAGCA GCCAGATGGC TCTACCTTGG GCGATCCAGC TAATGTGGAG
    TTCACAGAGA TCTGTATACA GCAGGGCTGG AAACCCCCAA GAGGCCGGTT CGATGTGCTG
    CCTCTCCTGC TCCAGGCCAA TGGCAATGAC CCTGAACTCT TTCAGATCCC CCCAGAGCTG
    GTGCTGGAAG TACCCATCAG GCACCCCAAG TTCGACTGGT TTAAGGACCT GGGGCTCAAA
    TGGTATGGCC TCCCCGCTGT GTCCAACATG CTGCTGGAGA TTGGGGGCCT GGAGTTCAGC
    GCCTGTCCCT TTAGTGGCTG GTACATGGGC ACGGAGATTG GCGTTCGTGA TTACTGTGAC
    AACTCTCGAT ACAACATCCT GGAGGAAGTA GCCAAGAAAA TGGATTTGGA CATGAGGAAG
    ACATCCTCCC TCTGGAAGGA CCAAGCCCTG GTGGAGATTA ACATTGCTGT CCTATACAGC
    TTCCAGAGTG ACAAGGTGAC CATTGTGGAC CACCACTCTG CCACCGAGTC CTTCATCAAA
    CACATGGAGA ATGAGTACCG CTGCAGAGGG GGCTGTCCCG CCGACTGGGT GTGGATTGTG
    CCCCCCATGT CGGGCAGCAT CACCCCTGTC TTCCACCAGG AGATGCTCAA CTACCGGCTC
    ACCCCGTCCT TTGAGTACCA GCCTGATCCA TGGAACACCC ACGTGTGGAA GGGCACCAAT
    GGGACCCCCA CGAAGCGGCG AGCCATTGGC TTCAAGAAAT TGGCAGAGGC CGTCAAGTTC
    TCAGCCAAGC TGATGGGACA GGCCATGGCC AAGAGAGTCA AAGCGACCAT TCTCTACGCC
    ACCGAGACAG GCAAATCCCA AGCCTATGCC AAGACCCTGT GCGAGATCTT CAAGCATGCC
    TTCGATGCCA AGGCTATGTC CATGGAGGAA TATGACATCG TGCACCTGGA GCACGAAGCC
    CTGGTCTTGG TGGTCACCAG CACCTTTGGC AATGGAGACC CCCCAGAGAA TGGGGAGAAA
    TTCGGCTGTG CTTTGATGGA GATGAGGCAC CCCAACTCTG TGCAAGAGGA GAGGAAGTAC
    CCGGAACCCT TGCGTTTCTT TCCCCGTAAA GGGCCTTCCC TCTCCCATGC TGACTCTGAA
    GCCCACAGTC TGGTTGCTGC CCGTGACAGC CAGCACAGGA GCTACAAGGT CCGATTCAAC
    AGCGTCTCCT CCTATTCTGA CTCCCGCAAG TCATCAGGCG ATGGACCAGA CCTCAGAGAC
    AACTTTGAAA GTACTGGACC GCTGGCCAAT GTGAGGTTCT CAGTCTTCGG CCTGGGCTCT
    CGGGCATACC CTCACTTCTG TGCCTTTGGG CACGCAGTGG ACACTCTCCT GGAGGAGCTG
    GGCGGGGAGA GGATTCTGAA GATGAGGGAG GGAGATGAGC TCTGCGGACA GGAAGAGGCT
    TTCAGGACCT GGGCTAAGAA AGTCTTCAAG GCAGCCTGTG ATGTGTTCTG TGTGGGGGAT
    GACGTCAACA TTGAGAAGGC GAACAATTCC CTCATTAGCA ATGACCGCAG CTGGAAGAGG
    AATAAATTCC GCCTCACTTA TGTGGCAGAA GCTCCAGAGC TGACCCAAGG TCTTTCCAAT
    GTTCACAAAA AGCGAGTCTC AGCCGCCCGA CTCCTCAGCC GCCAAAACCT GCAAAGTCCT
    AAATCCAGCC GATCGACCAT CTTCGTGCGT CTCCACACCA ACGGGAATCA GGAGTTGCAG
    TACCAGCCAG GGGACCACCT GGGTGTCTTC CCTGGCAACC ATGAGGACCT CGTGAATGCA
    CTCATTGAAC GGCTGGAGGA CGCGCCGCCT GCCAACCACG TGGTGAAGGT GGAAATGCTG
    GAGGAAAGGA ACACGGCTCT GGGTGTCATC AGTAATTGGA AGGATGAATC TCGCCTCCCA
    CCCTGCACCA TCTTCCAGGC CTTCAAGTAC TACCTGGACA TCACCACGCC GCCCACGCCC
    CTGCAGCTGC AGCAGTTCGC CTCCCTGGCC ACCAATGAGA AAGAGAAGCA GCGGCTGCTG
    GTCCTCAGCA AGGGGCTTCA GGAATATGAG GAATGGAAAT GGGGCAAGAA CCCCACAATG
    GTGGAGGTGC TGGAGGAGTT CCCGTCCATC CAGATGCCGG CCACACTTCT CCTCACACAG
    CTGTCGCTGC TACAACCTCG CTACTATTCC ATCAGCTCCT CTCCAGACAT GTACCCCGAC
    GAGGTGCACC TCACCGTGGC CATCGTGTCC TACCATACCC GAGATGGAGA AGGACCGGTT
    CACCACGGGG TGTGCTCCTC CTGGCTCAAC CGAATACAGG CTGACGATGT CGTCCCCTGC
    TTCGTGAGAG GTGCCCCTAG CTTCCACCTG CCTCGAAACC CCCAGGTGCC TTGCATCCTG
    GTTGGCCCAG GCACCGGCAT CGCTCCCTTC CGAAGTTTTT GGCAACAGCG ACAATTTGAC
    ATCCAACACA AAGGAATGAA TCCGTGCCCC ATGGTTCTGG TCTTCGGGTG TCGACAATCC
    AAGATAGATC ATATCTACAG AGAGGAGACC CTGCAGGCCA AGAACAAGGG CGTCTTCAGA
    GAGCTGTACA CTGCCTACTC CCGGGAACCC GACAGGCCAA AGAAATATGT ACAGGACGTG
    CTGCAGGAAC AGCTGGCCGA GTCTGTGTAC CGCGCCCTGA AGGAGCAAGG AGGCCATATT
    TATGTCTGCG GGGACGTTAC CATGGCCGCT GATGTCCTCA AAGCCATCCA GCGCATAATG
    ACCCAGCAGG GGAAACTCTC GGAGGAGGAT GCTGGTGTGT TCATCAGCAG GCTGAGGGAT
    GACAACCGGT ACCACGAGGA CATCTTTGGG GTCACCCTCA GAACATATGA AGTGACCAAC
    CGCCTTAGAT CTGAGTCCAT CGCCTTCATT GAAGAGAGCA AAAAAGACAC AGATGACAGG
    GTTTTCAGCT CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006530250.1
    CDS1113..5507
    Translation

    Target ORF information:

    RefSeq Version XM_006530187.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus nitric oxide synthase 1, neuronal (Nos1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006530187.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAAGAGC ACACGTTTGG GGTCCAGCAG ATCCAACCCA ACGTCATTTC TGTCCGTCTC 
    TTCAAACGCA AAGTGGGAGG TCTGGGCTTC CTGGTGAAGG AACGGGTCAG CAAGCCCCCG
    GTGATCATCT CAGATCTGAT CCGAGGAGGT GCCGCGGAGC AGAGCGGCCT TATCCAAGCC
    GGCGACATCA TTCTCGCCGT CAATGATCGG CCCCTGGTAG ACCTCAGCTA TGACAGCGCT
    CTGGAAGTTC TCAGGGGCAT TGCCTCTGAG ACCCATGTGG TCCTCATTCT GAGAGGCCCT
    GAGGGCTTTA CTACACACCT GGAGACCACC TTCACAGGGG ATGGAACCCC CAAGACCATC
    CGTGTGACCC AGCCCCTGGG GACACCCACC AAAGCTGTCG ATCTGTCTCG CCAGCCATCA
    GCCAGCAAAG ACCAGCCATT AGCAGTAGAC AGGGTCCCAG GTCCCAGTAA CGGACCTCAG
    CATGCCCAAG GCCGTGGACA GGGAGCCGGC TCAGTCTCCC AGGCTAATGG TGTGGCCATT
    GACCCCACAA TGAAAAATAC CAAGGCCAAC CTCCAGGACA GCGGAGAACA GGATGAACTG
    CTCAAAGAGA TAGAACCTGT GTTGAGCATC CTCACCGGTG GGGGAAAAGC AGTCAACAGA
    GGGGGACCAG CCAAAGCAGA GATGAAAGAC ACAGGAATCC AGGTGGACAG AGACCTCGAC
    GGCAAACTGC ACAAAGCTCC GCCCCTGGGC GGGGAGAATG ATCGAGTCTT CAATGATCTG
    TGGGGGAAGG GCAACGTCCC TGTGGTCCTC AACAACCCGT ATTCAGAGAA CGAACAGTCT
    CCCGCCTCGG GCAAACAGTC TCCTACCAAG AATGGCAGTC CTTCCAGGTG CCCTCGCTTC
    CTTAAGGTCA AGAACTGGGA GACAGATGTG GTCCTCACTG ACACCCTGCA CCTGAAGAGC
    ACGCTGGAAA CGGGGTGCAC AGAGCAGATC TGCATGGGCT CCATCATGCT GCCATCCCAT
    CACATACGGA AGTCAGAAGA TGTCCGCACC AAGGACCAGC TCTTCCCTCT AGCCAAAGAA
    TTTCTCGACC AATACTACTC CTCCATTAAG AGGTTTGGCT CCAAGGCCCA CATGGACAGG
    CTGGAGGAGG TGAACAAGGA GATCGAGAGC ACCAGCACCT ACCAGCTCAA GGACACGGAG
    CTCATCTATG GCGCCAAGCA TGCCTGGCGG AACGCCTCTC GCTGTGTGGG CAGGATCCAG
    TGGTCCAAGC TGCAGGTGTT CGATGCCCGA GACTGCACCA CAGCCCATGG GATGTTCAAC
    TACATCTGTA ACCACGTCAA GTACGCCACC AACAAAGGGA ACCTCAGGTC GGCCATCACT
    ATATTCCCTC AAAGGACTGA TGGCAAGCAT GACTTCCGAG TGTGGAACTC GCAGCTCATC
    CGCTATGCCG GCTACAAGCA GCCAGATGGC TCTACCTTGG GCGATCCAGC TAATGTGGAG
    TTCACAGAGA TCTGTATACA GCAGGGCTGG AAACCCCCAA GAGGCCGGTT CGATGTGCTG
    CCTCTCCTGC TCCAGGCCAA TGGCAATGAC CCTGAACTCT TTCAGATCCC CCCAGAGCTG
    GTGCTGGAAG TACCCATCAG GCACCCCAAG TTCGACTGGT TTAAGGACCT GGGGCTCAAA
    TGGTATGGCC TCCCCGCTGT GTCCAACATG CTGCTGGAGA TTGGGGGCCT GGAGTTCAGC
    GCCTGTCCCT TTAGTGGCTG GTACATGGGC ACGGAGATTG GCGTTCGTGA TTACTGTGAC
    AACTCTCGAT ACAACATCCT GGAGGAAGTA GCCAAGAAAA TGGATTTGGA CATGAGGAAG
    ACATCCTCCC TCTGGAAGGA CCAAGCCCTG GTGGAGATTA ACATTGCTGT CCTATACAGC
    TTCCAGAGTG ACAAGGTGAC CATTGTGGAC CACCACTCTG CCACCGAGTC CTTCATCAAA
    CACATGGAGA ATGAGTACCG CTGCAGAGGG GGCTGTCCCG CCGACTGGGT GTGGATTGTG
    CCCCCCATGT CGGGCAGCAT CACCCCTGTC TTCCACCAGG AGATGCTCAA CTACCGGCTC
    ACCCCGTCCT TTGAGTACCA GCCTGATCCA TGGAACACCC ACGTGTGGAA GGGCACCAAT
    GGGACCCCCA CGAAGCGGCG AGCCATTGGC TTCAAGAAAT TGGCAGAGGC CGTCAAGTTC
    TCAGCCAAGC TGATGGGACA GGCCATGGCC AAGAGAGTCA AAGCGACCAT TCTCTACGCC
    ACCGAGACAG GCAAATCCCA AGCCTATGCC AAGACCCTGT GCGAGATCTT CAAGCATGCC
    TTCGATGCCA AGGCTATGTC CATGGAGGAA TATGACATCG TGCACCTGGA GCACGAAGCC
    CTGGTCTTGG TGGTCACCAG CACCTTTGGC AATGGAGACC CCCCAGAGAA TGGGGAGAAA
    TTCGGCTGTG CTTTGATGGA GATGAGGCAC CCCAACTCTG TGCAAGAGGA GAGGAAGTAC
    CCGGAACCCT TGCGTTTCTT TCCCCGTAAA GGGCCTTCCC TCTCCCATGC TGACTCTGAA
    GCCCACAGTC TGGTTGCTGC CCGTGACAGC CAGCACAGGA GCTACAAGGT CCGATTCAAC
    AGCGTCTCCT CCTATTCTGA CTCCCGCAAG TCATCAGGCG ATGGACCAGA CCTCAGAGAC
    AACTTTGAAA GTACTGGACC GCTGGCCAAT GTGAGGTTCT CAGTCTTCGG CCTGGGCTCT
    CGGGCATACC CTCACTTCTG TGCCTTTGGG CACGCAGTGG ACACTCTCCT GGAGGAGCTG
    GGCGGGGAGA GGATTCTGAA GATGAGGGAG GGAGATGAGC TCTGCGGACA GGAAGAGGCT
    TTCAGGACCT GGGCTAAGAA AGTCTTCAAG GCAGCCTGTG ATGTGTTCTG TGTGGGGGAT
    GACGTCAACA TTGAGAAGGC GAACAATTCC CTCATTAGCA ATGACCGCAG CTGGAAGAGG
    AATAAATTCC GCCTCACTTA TGTGGCAGAA GCTCCAGAGC TGACCCAAGG TCTTTCCAAT
    GTTCACAAAA AGCGAGTCTC AGCCGCCCGA CTCCTCAGCC GCCAAAACCT GCAAAGTCCT
    AAATCCAGCC GATCGACCAT CTTCGTGCGT CTCCACACCA ACGGGAATCA GGAGTTGCAG
    TACCAGCCAG GGGACCACCT GGGTGTCTTC CCTGGCAACC ATGAGGACCT CGTGAATGCA
    CTCATTGAAC GGCTGGAGGA CGCGCCGCCT GCCAACCACG TGGTGAAGGT GGAAATGCTG
    GAGGAAAGGA ACACGGCTCT GGGTGTCATC AGTAATTGGA AGGATGAATC TCGCCTCCCA
    CCCTGCACCA TCTTCCAGGC CTTCAAGTAC TACCTGGACA TCACCACGCC GCCCACGCCC
    CTGCAGCTGC AGCAGTTCGC CTCCCTGGCC ACCAATGAGA AAGAGAAGCA GCGGCTGCTG
    GTCCTCAGCA AGGGGCTTCA GGAATATGAG GAATGGAAAT GGGGCAAGAA CCCCACAATG
    GTGGAGGTGC TGGAGGAGTT CCCGTCCATC CAGATGCCGG CCACACTTCT CCTCACACAG
    CTGTCGCTGC TACAACCTCG CTACTATTCC ATCAGCTCCT CTCCAGACAT GTACCCCGAC
    GAGGTGCACC TCACCGTGGC CATCGTGTCC TACCATACCC GAGATGGAGA AGGACCGGTT
    CACCACGGGG TGTGCTCCTC CTGGCTCAAC CGAATACAGG CTGACGATGT CGTCCCCTGC
    TTCGTGAGAG GTGCCCCTAG CTTCCACCTG CCTCGAAACC CCCAGGTGCC TTGCATCCTG
    GTTGGCCCAG GCACCGGCAT CGCTCCCTTC CGAAGTTTTT GGCAACAGCG ACAATTTGAC
    ATCCAACACA AAGGAATGAA TCCGTGCCCC ATGGTTCTGG TCTTCGGGTG TCGACAATCC
    AAGATAGATC ATATCTACAG AGAGGAGACC CTGCAGGCCA AGAACAAGGG CGTCTTCAGA
    GAGCTGTACA CTGCCTACTC CCGGGAACCC GACAGGCCAA AGAAATATGT ACAGGACGTG
    CTGCAGGAAC AGCTGGCCGA GTCTGTGTAC CGCGCCCTGA AGGAGCAAGG AGGCCATATT
    TATGTCTGCG GGGACGTTAC CATGGCCGCT GATGTCCTCA AAGCCATCCA GCGCATAATG
    ACCCAGCAGG GGAAACTCTC GGAGGAGGAT GCTGGTGTGT TCATCAGCAG GCTGAGGGAT
    GACAACCGGT ACCACGAGGA CATCTTTGGG GTCACCCTCA GAACATATGA AGTGACCAAC
    CGCCTTAGAT CTGAGTCCAT CGCCTTCATT GAAGAGAGCA AAAAAGACAC AGATGACAGG
    GTTTTCAGCT CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu35811
    Clone ID Related Accession (Same CDS sequence) XM_006530189.3 , XM_006530188.3 , XM_006530187.3 , XM_006530190.1
    Accession Version XM_006530190.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4395bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product nitric oxide synthase, brain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAAGAGC ACACGTTTGG GGTCCAGCAG ATCCAACCCA ACGTCATTTC TGTCCGTCTC 
    TTCAAACGCA AAGTGGGAGG TCTGGGCTTC CTGGTGAAGG AACGGGTCAG CAAGCCCCCG
    GTGATCATCT CAGATCTGAT CCGAGGAGGT GCCGCGGAGC AGAGCGGCCT TATCCAAGCC
    GGCGACATCA TTCTCGCCGT CAATGATCGG CCCCTGGTAG ACCTCAGCTA TGACAGCGCT
    CTGGAAGTTC TCAGGGGCAT TGCCTCTGAG ACCCATGTGG TCCTCATTCT GAGAGGCCCT
    GAGGGCTTTA CTACACACCT GGAGACCACC TTCACAGGGG ATGGAACCCC CAAGACCATC
    CGTGTGACCC AGCCCCTGGG GACACCCACC AAAGCTGTCG ATCTGTCTCG CCAGCCATCA
    GCCAGCAAAG ACCAGCCATT AGCAGTAGAC AGGGTCCCAG GTCCCAGTAA CGGACCTCAG
    CATGCCCAAG GCCGTGGACA GGGAGCCGGC TCAGTCTCCC AGGCTAATGG TGTGGCCATT
    GACCCCACAA TGAAAAATAC CAAGGCCAAC CTCCAGGACA GCGGAGAACA GGATGAACTG
    CTCAAAGAGA TAGAACCTGT GTTGAGCATC CTCACCGGTG GGGGAAAAGC AGTCAACAGA
    GGGGGACCAG CCAAAGCAGA GATGAAAGAC ACAGGAATCC AGGTGGACAG AGACCTCGAC
    GGCAAACTGC ACAAAGCTCC GCCCCTGGGC GGGGAGAATG ATCGAGTCTT CAATGATCTG
    TGGGGGAAGG GCAACGTCCC TGTGGTCCTC AACAACCCGT ATTCAGAGAA CGAACAGTCT
    CCCGCCTCGG GCAAACAGTC TCCTACCAAG AATGGCAGTC CTTCCAGGTG CCCTCGCTTC
    CTTAAGGTCA AGAACTGGGA GACAGATGTG GTCCTCACTG ACACCCTGCA CCTGAAGAGC
    ACGCTGGAAA CGGGGTGCAC AGAGCAGATC TGCATGGGCT CCATCATGCT GCCATCCCAT
    CACATACGGA AGTCAGAAGA TGTCCGCACC AAGGACCAGC TCTTCCCTCT AGCCAAAGAA
    TTTCTCGACC AATACTACTC CTCCATTAAG AGGTTTGGCT CCAAGGCCCA CATGGACAGG
    CTGGAGGAGG TGAACAAGGA GATCGAGAGC ACCAGCACCT ACCAGCTCAA GGACACGGAG
    CTCATCTATG GCGCCAAGCA TGCCTGGCGG AACGCCTCTC GCTGTGTGGG CAGGATCCAG
    TGGTCCAAGC TGCAGGTGTT CGATGCCCGA GACTGCACCA CAGCCCATGG GATGTTCAAC
    TACATCTGTA ACCACGTCAA GTACGCCACC AACAAAGGGA ACCTCAGGTC GGCCATCACT
    ATATTCCCTC AAAGGACTGA TGGCAAGCAT GACTTCCGAG TGTGGAACTC GCAGCTCATC
    CGCTATGCCG GCTACAAGCA GCCAGATGGC TCTACCTTGG GCGATCCAGC TAATGTGGAG
    TTCACAGAGA TCTGTATACA GCAGGGCTGG AAACCCCCAA GAGGCCGGTT CGATGTGCTG
    CCTCTCCTGC TCCAGGCCAA TGGCAATGAC CCTGAACTCT TTCAGATCCC CCCAGAGCTG
    GTGCTGGAAG TACCCATCAG GCACCCCAAG TTCGACTGGT TTAAGGACCT GGGGCTCAAA
    TGGTATGGCC TCCCCGCTGT GTCCAACATG CTGCTGGAGA TTGGGGGCCT GGAGTTCAGC
    GCCTGTCCCT TTAGTGGCTG GTACATGGGC ACGGAGATTG GCGTTCGTGA TTACTGTGAC
    AACTCTCGAT ACAACATCCT GGAGGAAGTA GCCAAGAAAA TGGATTTGGA CATGAGGAAG
    ACATCCTCCC TCTGGAAGGA CCAAGCCCTG GTGGAGATTA ACATTGCTGT CCTATACAGC
    TTCCAGAGTG ACAAGGTGAC CATTGTGGAC CACCACTCTG CCACCGAGTC CTTCATCAAA
    CACATGGAGA ATGAGTACCG CTGCAGAGGG GGCTGTCCCG CCGACTGGGT GTGGATTGTG
    CCCCCCATGT CGGGCAGCAT CACCCCTGTC TTCCACCAGG AGATGCTCAA CTACCGGCTC
    ACCCCGTCCT TTGAGTACCA GCCTGATCCA TGGAACACCC ACGTGTGGAA GGGCACCAAT
    GGGACCCCCA CGAAGCGGCG AGCCATTGGC TTCAAGAAAT TGGCAGAGGC CGTCAAGTTC
    TCAGCCAAGC TGATGGGACA GGCCATGGCC AAGAGAGTCA AAGCGACCAT TCTCTACGCC
    ACCGAGACAG GCAAATCCCA AGCCTATGCC AAGACCCTGT GCGAGATCTT CAAGCATGCC
    TTCGATGCCA AGGCTATGTC CATGGAGGAA TATGACATCG TGCACCTGGA GCACGAAGCC
    CTGGTCTTGG TGGTCACCAG CACCTTTGGC AATGGAGACC CCCCAGAGAA TGGGGAGAAA
    TTCGGCTGTG CTTTGATGGA GATGAGGCAC CCCAACTCTG TGCAAGAGGA GAGGAAGTAC
    CCGGAACCCT TGCGTTTCTT TCCCCGTAAA GGGCCTTCCC TCTCCCATGC TGACTCTGAA
    GCCCACAGTC TGGTTGCTGC CCGTGACAGC CAGCACAGGA GCTACAAGGT CCGATTCAAC
    AGCGTCTCCT CCTATTCTGA CTCCCGCAAG TCATCAGGCG ATGGACCAGA CCTCAGAGAC
    AACTTTGAAA GTACTGGACC GCTGGCCAAT GTGAGGTTCT CAGTCTTCGG CCTGGGCTCT
    CGGGCATACC CTCACTTCTG TGCCTTTGGG CACGCAGTGG ACACTCTCCT GGAGGAGCTG
    GGCGGGGAGA GGATTCTGAA GATGAGGGAG GGAGATGAGC TCTGCGGACA GGAAGAGGCT
    TTCAGGACCT GGGCTAAGAA AGTCTTCAAG GCAGCCTGTG ATGTGTTCTG TGTGGGGGAT
    GACGTCAACA TTGAGAAGGC GAACAATTCC CTCATTAGCA ATGACCGCAG CTGGAAGAGG
    AATAAATTCC GCCTCACTTA TGTGGCAGAA GCTCCAGAGC TGACCCAAGG TCTTTCCAAT
    GTTCACAAAA AGCGAGTCTC AGCCGCCCGA CTCCTCAGCC GCCAAAACCT GCAAAGTCCT
    AAATCCAGCC GATCGACCAT CTTCGTGCGT CTCCACACCA ACGGGAATCA GGAGTTGCAG
    TACCAGCCAG GGGACCACCT GGGTGTCTTC CCTGGCAACC ATGAGGACCT CGTGAATGCA
    CTCATTGAAC GGCTGGAGGA CGCGCCGCCT GCCAACCACG TGGTGAAGGT GGAAATGCTG
    GAGGAAAGGA ACACGGCTCT GGGTGTCATC AGTAATTGGA AGGATGAATC TCGCCTCCCA
    CCCTGCACCA TCTTCCAGGC CTTCAAGTAC TACCTGGACA TCACCACGCC GCCCACGCCC
    CTGCAGCTGC AGCAGTTCGC CTCCCTGGCC ACCAATGAGA AAGAGAAGCA GCGGCTGCTG
    GTCCTCAGCA AGGGGCTTCA GGAATATGAG GAATGGAAAT GGGGCAAGAA CCCCACAATG
    GTGGAGGTGC TGGAGGAGTT CCCGTCCATC CAGATGCCGG CCACACTTCT CCTCACACAG
    CTGTCGCTGC TACAACCTCG CTACTATTCC ATCAGCTCCT CTCCAGACAT GTACCCCGAC
    GAGGTGCACC TCACCGTGGC CATCGTGTCC TACCATACCC GAGATGGAGA AGGACCGGTT
    CACCACGGGG TGTGCTCCTC CTGGCTCAAC CGAATACAGG CTGACGATGT CGTCCCCTGC
    TTCGTGAGAG GTGCCCCTAG CTTCCACCTG CCTCGAAACC CCCAGGTGCC TTGCATCCTG
    GTTGGCCCAG GCACCGGCAT CGCTCCCTTC CGAAGTTTTT GGCAACAGCG ACAATTTGAC
    ATCCAACACA AAGGAATGAA TCCGTGCCCC ATGGTTCTGG TCTTCGGGTG TCGACAATCC
    AAGATAGATC ATATCTACAG AGAGGAGACC CTGCAGGCCA AGAACAAGGG CGTCTTCAGA
    GAGCTGTACA CTGCCTACTC CCGGGAACCC GACAGGCCAA AGAAATATGT ACAGGACGTG
    CTGCAGGAAC AGCTGGCCGA GTCTGTGTAC CGCGCCCTGA AGGAGCAAGG AGGCCATATT
    TATGTCTGCG GGGACGTTAC CATGGCCGCT GATGTCCTCA AAGCCATCCA GCGCATAATG
    ACCCAGCAGG GGAAACTCTC GGAGGAGGAT GCTGGTGTGT TCATCAGCAG GCTGAGGGAT
    GACAACCGGT ACCACGAGGA CATCTTTGGG GTCACCCTCA GAACATATGA AGTGACCAAC
    CGCCTTAGAT CTGAGTCCAT CGCCTTCATT GAAGAGAGCA AAAAAGACAC AGATGACAGG
    GTTTTCAGCT CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006530253.1
    CDS529..4923
    Translation

    Target ORF information:

    RefSeq Version XM_006530190.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus nitric oxide synthase 1, neuronal (Nos1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006530190.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAAGAGC ACACGTTTGG GGTCCAGCAG ATCCAACCCA ACGTCATTTC TGTCCGTCTC 
    TTCAAACGCA AAGTGGGAGG TCTGGGCTTC CTGGTGAAGG AACGGGTCAG CAAGCCCCCG
    GTGATCATCT CAGATCTGAT CCGAGGAGGT GCCGCGGAGC AGAGCGGCCT TATCCAAGCC
    GGCGACATCA TTCTCGCCGT CAATGATCGG CCCCTGGTAG ACCTCAGCTA TGACAGCGCT
    CTGGAAGTTC TCAGGGGCAT TGCCTCTGAG ACCCATGTGG TCCTCATTCT GAGAGGCCCT
    GAGGGCTTTA CTACACACCT GGAGACCACC TTCACAGGGG ATGGAACCCC CAAGACCATC
    CGTGTGACCC AGCCCCTGGG GACACCCACC AAAGCTGTCG ATCTGTCTCG CCAGCCATCA
    GCCAGCAAAG ACCAGCCATT AGCAGTAGAC AGGGTCCCAG GTCCCAGTAA CGGACCTCAG
    CATGCCCAAG GCCGTGGACA GGGAGCCGGC TCAGTCTCCC AGGCTAATGG TGTGGCCATT
    GACCCCACAA TGAAAAATAC CAAGGCCAAC CTCCAGGACA GCGGAGAACA GGATGAACTG
    CTCAAAGAGA TAGAACCTGT GTTGAGCATC CTCACCGGTG GGGGAAAAGC AGTCAACAGA
    GGGGGACCAG CCAAAGCAGA GATGAAAGAC ACAGGAATCC AGGTGGACAG AGACCTCGAC
    GGCAAACTGC ACAAAGCTCC GCCCCTGGGC GGGGAGAATG ATCGAGTCTT CAATGATCTG
    TGGGGGAAGG GCAACGTCCC TGTGGTCCTC AACAACCCGT ATTCAGAGAA CGAACAGTCT
    CCCGCCTCGG GCAAACAGTC TCCTACCAAG AATGGCAGTC CTTCCAGGTG CCCTCGCTTC
    CTTAAGGTCA AGAACTGGGA GACAGATGTG GTCCTCACTG ACACCCTGCA CCTGAAGAGC
    ACGCTGGAAA CGGGGTGCAC AGAGCAGATC TGCATGGGCT CCATCATGCT GCCATCCCAT
    CACATACGGA AGTCAGAAGA TGTCCGCACC AAGGACCAGC TCTTCCCTCT AGCCAAAGAA
    TTTCTCGACC AATACTACTC CTCCATTAAG AGGTTTGGCT CCAAGGCCCA CATGGACAGG
    CTGGAGGAGG TGAACAAGGA GATCGAGAGC ACCAGCACCT ACCAGCTCAA GGACACGGAG
    CTCATCTATG GCGCCAAGCA TGCCTGGCGG AACGCCTCTC GCTGTGTGGG CAGGATCCAG
    TGGTCCAAGC TGCAGGTGTT CGATGCCCGA GACTGCACCA CAGCCCATGG GATGTTCAAC
    TACATCTGTA ACCACGTCAA GTACGCCACC AACAAAGGGA ACCTCAGGTC GGCCATCACT
    ATATTCCCTC AAAGGACTGA TGGCAAGCAT GACTTCCGAG TGTGGAACTC GCAGCTCATC
    CGCTATGCCG GCTACAAGCA GCCAGATGGC TCTACCTTGG GCGATCCAGC TAATGTGGAG
    TTCACAGAGA TCTGTATACA GCAGGGCTGG AAACCCCCAA GAGGCCGGTT CGATGTGCTG
    CCTCTCCTGC TCCAGGCCAA TGGCAATGAC CCTGAACTCT TTCAGATCCC CCCAGAGCTG
    GTGCTGGAAG TACCCATCAG GCACCCCAAG TTCGACTGGT TTAAGGACCT GGGGCTCAAA
    TGGTATGGCC TCCCCGCTGT GTCCAACATG CTGCTGGAGA TTGGGGGCCT GGAGTTCAGC
    GCCTGTCCCT TTAGTGGCTG GTACATGGGC ACGGAGATTG GCGTTCGTGA TTACTGTGAC
    AACTCTCGAT ACAACATCCT GGAGGAAGTA GCCAAGAAAA TGGATTTGGA CATGAGGAAG
    ACATCCTCCC TCTGGAAGGA CCAAGCCCTG GTGGAGATTA ACATTGCTGT CCTATACAGC
    TTCCAGAGTG ACAAGGTGAC CATTGTGGAC CACCACTCTG CCACCGAGTC CTTCATCAAA
    CACATGGAGA ATGAGTACCG CTGCAGAGGG GGCTGTCCCG CCGACTGGGT GTGGATTGTG
    CCCCCCATGT CGGGCAGCAT CACCCCTGTC TTCCACCAGG AGATGCTCAA CTACCGGCTC
    ACCCCGTCCT TTGAGTACCA GCCTGATCCA TGGAACACCC ACGTGTGGAA GGGCACCAAT
    GGGACCCCCA CGAAGCGGCG AGCCATTGGC TTCAAGAAAT TGGCAGAGGC CGTCAAGTTC
    TCAGCCAAGC TGATGGGACA GGCCATGGCC AAGAGAGTCA AAGCGACCAT TCTCTACGCC
    ACCGAGACAG GCAAATCCCA AGCCTATGCC AAGACCCTGT GCGAGATCTT CAAGCATGCC
    TTCGATGCCA AGGCTATGTC CATGGAGGAA TATGACATCG TGCACCTGGA GCACGAAGCC
    CTGGTCTTGG TGGTCACCAG CACCTTTGGC AATGGAGACC CCCCAGAGAA TGGGGAGAAA
    TTCGGCTGTG CTTTGATGGA GATGAGGCAC CCCAACTCTG TGCAAGAGGA GAGGAAGTAC
    CCGGAACCCT TGCGTTTCTT TCCCCGTAAA GGGCCTTCCC TCTCCCATGC TGACTCTGAA
    GCCCACAGTC TGGTTGCTGC CCGTGACAGC CAGCACAGGA GCTACAAGGT CCGATTCAAC
    AGCGTCTCCT CCTATTCTGA CTCCCGCAAG TCATCAGGCG ATGGACCAGA CCTCAGAGAC
    AACTTTGAAA GTACTGGACC GCTGGCCAAT GTGAGGTTCT CAGTCTTCGG CCTGGGCTCT
    CGGGCATACC CTCACTTCTG TGCCTTTGGG CACGCAGTGG ACACTCTCCT GGAGGAGCTG
    GGCGGGGAGA GGATTCTGAA GATGAGGGAG GGAGATGAGC TCTGCGGACA GGAAGAGGCT
    TTCAGGACCT GGGCTAAGAA AGTCTTCAAG GCAGCCTGTG ATGTGTTCTG TGTGGGGGAT
    GACGTCAACA TTGAGAAGGC GAACAATTCC CTCATTAGCA ATGACCGCAG CTGGAAGAGG
    AATAAATTCC GCCTCACTTA TGTGGCAGAA GCTCCAGAGC TGACCCAAGG TCTTTCCAAT
    GTTCACAAAA AGCGAGTCTC AGCCGCCCGA CTCCTCAGCC GCCAAAACCT GCAAAGTCCT
    AAATCCAGCC GATCGACCAT CTTCGTGCGT CTCCACACCA ACGGGAATCA GGAGTTGCAG
    TACCAGCCAG GGGACCACCT GGGTGTCTTC CCTGGCAACC ATGAGGACCT CGTGAATGCA
    CTCATTGAAC GGCTGGAGGA CGCGCCGCCT GCCAACCACG TGGTGAAGGT GGAAATGCTG
    GAGGAAAGGA ACACGGCTCT GGGTGTCATC AGTAATTGGA AGGATGAATC TCGCCTCCCA
    CCCTGCACCA TCTTCCAGGC CTTCAAGTAC TACCTGGACA TCACCACGCC GCCCACGCCC
    CTGCAGCTGC AGCAGTTCGC CTCCCTGGCC ACCAATGAGA AAGAGAAGCA GCGGCTGCTG
    GTCCTCAGCA AGGGGCTTCA GGAATATGAG GAATGGAAAT GGGGCAAGAA CCCCACAATG
    GTGGAGGTGC TGGAGGAGTT CCCGTCCATC CAGATGCCGG CCACACTTCT CCTCACACAG
    CTGTCGCTGC TACAACCTCG CTACTATTCC ATCAGCTCCT CTCCAGACAT GTACCCCGAC
    GAGGTGCACC TCACCGTGGC CATCGTGTCC TACCATACCC GAGATGGAGA AGGACCGGTT
    CACCACGGGG TGTGCTCCTC CTGGCTCAAC CGAATACAGG CTGACGATGT CGTCCCCTGC
    TTCGTGAGAG GTGCCCCTAG CTTCCACCTG CCTCGAAACC CCCAGGTGCC TTGCATCCTG
    GTTGGCCCAG GCACCGGCAT CGCTCCCTTC CGAAGTTTTT GGCAACAGCG ACAATTTGAC
    ATCCAACACA AAGGAATGAA TCCGTGCCCC ATGGTTCTGG TCTTCGGGTG TCGACAATCC
    AAGATAGATC ATATCTACAG AGAGGAGACC CTGCAGGCCA AGAACAAGGG CGTCTTCAGA
    GAGCTGTACA CTGCCTACTC CCGGGAACCC GACAGGCCAA AGAAATATGT ACAGGACGTG
    CTGCAGGAAC AGCTGGCCGA GTCTGTGTAC CGCGCCCTGA AGGAGCAAGG AGGCCATATT
    TATGTCTGCG GGGACGTTAC CATGGCCGCT GATGTCCTCA AAGCCATCCA GCGCATAATG
    ACCCAGCAGG GGAAACTCTC GGAGGAGGAT GCTGGTGTGT TCATCAGCAG GCTGAGGGAT
    GACAACCGGT ACCACGAGGA CATCTTTGGG GTCACCCTCA GAACATATGA AGTGACCAAC
    CGCCTTAGAT CTGAGTCCAT CGCCTTCATT GAAGAGAGCA AAAAAGACAC AGATGACAGG
    GTTTTCAGCT CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu35812
    Clone ID Related Accession (Same CDS sequence) XM_006530191.3
    Accession Version XM_006530191.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4392bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product nitric oxide synthase, brain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006530191.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAAGAGC ACACGTTTGG GGTCCAGCAG ATCCAACCCA ACGTCATTTC TGTCCGTCTC 
    TTCAAACGCA AAGTGGGAGG TCTGGGCTTC CTGGTGAAGG AACGGGTCAG CAAGCCCCCG
    GTGATCATCT CAGATCTGAT CCGAGGAGGT GCCGCGGAGC AGAGCGGCCT TATCCAAGCC
    GGCGACATCA TTCTCGCCGT CAATGATCGG CCCCTGGTAG ACCTCAGCTA TGACAGCGCT
    CTGGAAGTTC TCAGGGGCAT TGCCTCTGAG ACCCATGTGG TCCTCATTCT GAGAGGCCCT
    GAGGGCTTTA CTACACACCT GGAGACCACC TTCACAGGGG ATGGAACCCC CAAGACCATC
    CGTGTGACCC AGCCCCTGGG GACACCCACC AAAGCTGTCG ATCTGTCTCG CCAGCCATCA
    GCCAGCAAAG ACCAGCCATT AGCAGTAGAC AGGGTCCCAG GTCCCAGTAA CGGACCTCAG
    CATGCCCAAG GCCGTGGACA GGGAGCCGGC TCAGTCTCCC AGGCTAATGG TGTGGCCATT
    GACCCCACAA TGAAAAATAC CAAGGCCAAC CTCCAGGACA GCGGAGAACA GGATGAACTG
    CTCAAAGAGA TAGAACCTGT GTTGAGCATC CTCACCGGTG GGGGAAAAGC AGTCAACAGA
    GGGGGACCAG CCAAAGCAGA GATGAAAGAC ACAGGAATCC AGGTGGACAG AGACCTCGAC
    GGCAAACTGC ACAAAGCTCC GCCCCTGGGC GGGGAGAATG ATCGAGTCTT CAATGATCTG
    TGGGGGAAGG GCAACGTCCC TGTGGTCCTC AACAACCCGT ATTCAGAGAA CGAACAGTCT
    CCCGCCTCGG GCAAACAGTC TCCTACCAAG AATGGCAGTC CTTCCAGGTG CCCTCGCTTC
    CTTAAGGTCA AGAACTGGGA GACAGATGTG GTCCTCACTG ACACCCTGCA CCTGAAGAGC
    ACGCTGGAAA CGGGGTGCAC AGAGCAGATC TGCATGGGCT CCATCATGCT GCCATCCCAT
    CACATACGGA AGTCAGAAGA TGTCCGCACC AAGGACCAGC TCTTCCCTCT AGCCAAAGAA
    TTTCTCGACC AATACTACTC CTCCATTAAG AGGTTTGGCT CCAAGGCCCA CATGGACAGG
    CTGGAGGAGG TGAACAAGGA GATCGAGAGC ACCAGCACCT ACCAGCTCAA GGACACGGAG
    CTCATCTATG GCGCCAAGCA TGCCTGGCGG AACGCCTCTC GCTGTGTGGG CAGGATCCAG
    TGGTCCAAGC TGCAGGTGTT CGATGCCCGA GACTGCACCA CAGCCCATGG GATGTTCAAC
    TACATCTGTA ACCACGTCAA GTACGCCACC AACAAAGGGA ACCTCAGGTC GGCCATCACT
    ATATTCCCTC AAAGGACTGA TGGCAAGCAT GACTTCCGAG TGTGGAACTC GCAGCTCATC
    CGCTATGCCG GCTACAAGCA GCCAGATGGC TCTACCTTGG GCGATCCAGC TAATGTGGAG
    TTCACAGAGA TCTGTATACA GCAGGGCTGG AAACCCCCAA GAGGCCGGTT CGATGTGCTG
    CCTCTCCTGC TCCAGGCCAA TGGCAATGAC CCTGAACTCT TTCAGATCCC CCCAGAGCTG
    GTGCTGGAAG TACCCATCAG GCACCCCAAG TTCGACTGGT TTAAGGACCT GGGGCTCAAA
    TGGTATGGCC TCCCCGCTGT GTCCAACATG CTGCTGGAGA TTGGGGGCCT GGAGTTCAGC
    GCCTGTCCCT TTAGTGGCTG GTACATGGGC ACGGAGATTG GCGTTCGTGA TTACTGTGAC
    AACTCTCGAT ACAACATCCT GGAGGAAGTA GCCAAGAAAA TGGATTTGGA CATGAGGAAG
    ACATCCTCCC TCTGGAAGGA CCAAGCCCTG GTGGAGATTA ACATTGCTGT CCTATACAGC
    TTCCAGAGTG ACAAGGTGAC CATTGTGGAC CACCACTCTG CCACCGAGTC CTTCATCAAA
    CACATGGAGA ATGAGTACCG CTGCAGAGGG GGCTGTCCCG CCGACTGGGT GTGGATTGTG
    CCCCCCATGT CGGGCAGCAT CACCCCTGTC TTCCACCAGG AGATGCTCAA CTACCGGCTC
    ACCCCGTCCT TTGAGTACCA GCCTGATCCA TGGAACACCC ACGTGTGGAA GGGCACCAAT
    GGGACCCCCA CGAAGCGGCG AGCCATTGGC TTCAAGAAAT TGGCAGAGGC CGTCAAGTTC
    TCAGCCAAGC TGATGGGACA GGCCATGGCC AAGAGAGTCA AAGCGACCAT TCTCTACGCC
    ACCGAGACAG GCAAATCCCA AGCCTATGCC AAGACCCTGT GCGAGATCTT CAAGCATGCC
    TTCGATGCCA AGGCTATGTC CATGGAGGAA TATGACATCG TGCACCTGGA GCACGAAGCC
    CTGGTCTTGG TGGTCACCAG CACCTTTGGC AATGGAGACC CCCCAGAGAA TGGGGAGAAA
    TTCGGCTGTG CTTTGATGGA GATGAGGCAC CCCAACTCTG TGCAAGAGGA GAGGAAGTAC
    CCGGAACCCT TGCGTTTCTT TCCCCGTAAA GGGCCTTCCC TCTCCCATGC TGACTCTGAA
    GCCCACAGTC TGGTTGCTGC CCGTGACAGC CAGCACAGGA GCTACAAGGT CCGATTCAAC
    AGCGTCTCCT CCTATTCTGA CTCCCGCAAG TCATCAGGCG ATGGACCAGA CCTCAGAGAC
    AACTTTGAAA GTACTGGACC GCTGGCCAAT GTGAGGTTCT CAGTCTTCGG CCTGGGCTCT
    CGGGCATACC CTCACTTCTG TGCCTTTGGG CACGCAGTGG ACACTCTCCT GGAGGAGCTG
    GGCGGGGAGA GGATTCTGAA GATGAGGGAG GGAGATGAGC TCTGCGGACA GGAAGAGGCT
    TTCAGGACCT GGGCTAAGAA AGTCTTCAAG GCAGCCTGTG ATGTGTTCTG TGTGGGGGAT
    GACGTCAACA TTGAGAAGGC GAACAATTCC CTCATTAGCA ATGACCGCAG CTGGAAGAGG
    AATAAATTCC GCCTCACTTA TGTGGCAGAA GCTCCAGAGC TGACCCAAGG TCTTTCCAAT
    GTTCACAAAA AGCGAGTCTC AGCCGCCCGA CTCCTCAGCC GCCAAAACCT GCAAAGTCCT
    AAATCCAGCC GATCGACCAT CTTCGTGCGT CTCCACACCA ACGGGAATCA GGAGTTGCAG
    TACCAGCCAG GGGACCACCT GGGTGTCTTC CCTGGCAACC ATGAGGACCT CGTGAATGCA
    CTCATTGAAC GGCTGGAGGA CGCGCCGCCT GCCAACCACG TGGTGAAGGT GGAAATGCTG
    GAGGAAAGGA ACACGGCTCT GGGTGTCATC AGTAATTGGA AGGATGAATC TCGCCTCCCA
    CCCTGCACCA TCTTCCAGGC CTTCAAGTAC TACCTGGACA TCACCACGCC GCCCACGCCC
    CTGCAGCTGC AGCAGTTCGC CTCCCTGGCC ACCAATGAGA AAGAGAAGCA GCGGCTGCTG
    GTCCTCAGCA AGGGGCTTCA GGAATATGAG GAATGGAAAT GGGGCAAGAA CCCCACAATG
    GTGGAGGTGC TGGAGGAGTT CCCGTCCATC CAGATGCCGG CCACACTTCT CCTCACACAG
    CTGTCGCTGC TACAACCTCG CTACTATTCC ATCAGCTCCT CTCCAGACAT GTACCCCGAC
    GAGGTGCACC TCACCGTGGC CATCGTGTCC TACCATACCC GAGATGGAGA AGGACCGGTT
    CACCACGGGG TGTGCTCCTC CTGGCTCAAC CGAATACAGG CTGACGATGT CGTCCCCTGC
    TTCGTGAGAG GTGCCCCTAG CTTCCACCTG CCTCGAAACC CCCAGGTGCC TTGCATCCTG
    GTTGGCCCAG GCACCGGCAT CGCTCCCTTC CGAAGTTTTT GGCAACAGCG ACAATTTGAC
    ATCCAACACA AAGGAATGAA TCCGTGCCCC ATGGTTCTGG TCTTCGGGTG TCGACAATCC
    AAGATAGATC ATATCTACAG AGAGGAGACC CTGCAGGCCA AGAACAAGGG CGTCTTCAGA
    GAGCTGTACA CTGCCTACTC CCGGGAACCC GACAGGCCAA AGAAATATGT ACAGGACGTG
    CTGCAGGAAC AGCTGGCCGA GTCTGTGTAC CGCGCCCTGA AGGAGCAAGG AGGCCATATT
    TATGTCTGCG GGGACGTTAC CATGGCCGCT GATGTCCTCA AAGCCATCCA GCGCATAATG
    ACCCAGCAGG GGAAACTCTC GGAGGAGGAT GCTGGTGTGT TCATCAGCAG GCTGAGGGAT
    GACAACCGGT ACCACGAGGA CATCTTTGGG GTCACCCTCA GAACATATGA AGTGACCAAC
    CGCCTTAGAT CTGAGTCCAT CGCCTTCATT GAAGAGAGCA AAAAAGACAC AGATGAGGTT
    TTCAGCTCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006530254.1
    CDS1112..5503
    Translation

    Target ORF information:

    RefSeq Version XM_006530191.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus nitric oxide synthase 1, neuronal (Nos1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006530191.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAAGAGC ACACGTTTGG GGTCCAGCAG ATCCAACCCA ACGTCATTTC TGTCCGTCTC 
    TTCAAACGCA AAGTGGGAGG TCTGGGCTTC CTGGTGAAGG AACGGGTCAG CAAGCCCCCG
    GTGATCATCT CAGATCTGAT CCGAGGAGGT GCCGCGGAGC AGAGCGGCCT TATCCAAGCC
    GGCGACATCA TTCTCGCCGT CAATGATCGG CCCCTGGTAG ACCTCAGCTA TGACAGCGCT
    CTGGAAGTTC TCAGGGGCAT TGCCTCTGAG ACCCATGTGG TCCTCATTCT GAGAGGCCCT
    GAGGGCTTTA CTACACACCT GGAGACCACC TTCACAGGGG ATGGAACCCC CAAGACCATC
    CGTGTGACCC AGCCCCTGGG GACACCCACC AAAGCTGTCG ATCTGTCTCG CCAGCCATCA
    GCCAGCAAAG ACCAGCCATT AGCAGTAGAC AGGGTCCCAG GTCCCAGTAA CGGACCTCAG
    CATGCCCAAG GCCGTGGACA GGGAGCCGGC TCAGTCTCCC AGGCTAATGG TGTGGCCATT
    GACCCCACAA TGAAAAATAC CAAGGCCAAC CTCCAGGACA GCGGAGAACA GGATGAACTG
    CTCAAAGAGA TAGAACCTGT GTTGAGCATC CTCACCGGTG GGGGAAAAGC AGTCAACAGA
    GGGGGACCAG CCAAAGCAGA GATGAAAGAC ACAGGAATCC AGGTGGACAG AGACCTCGAC
    GGCAAACTGC ACAAAGCTCC GCCCCTGGGC GGGGAGAATG ATCGAGTCTT CAATGATCTG
    TGGGGGAAGG GCAACGTCCC TGTGGTCCTC AACAACCCGT ATTCAGAGAA CGAACAGTCT
    CCCGCCTCGG GCAAACAGTC TCCTACCAAG AATGGCAGTC CTTCCAGGTG CCCTCGCTTC
    CTTAAGGTCA AGAACTGGGA GACAGATGTG GTCCTCACTG ACACCCTGCA CCTGAAGAGC
    ACGCTGGAAA CGGGGTGCAC AGAGCAGATC TGCATGGGCT CCATCATGCT GCCATCCCAT
    CACATACGGA AGTCAGAAGA TGTCCGCACC AAGGACCAGC TCTTCCCTCT AGCCAAAGAA
    TTTCTCGACC AATACTACTC CTCCATTAAG AGGTTTGGCT CCAAGGCCCA CATGGACAGG
    CTGGAGGAGG TGAACAAGGA GATCGAGAGC ACCAGCACCT ACCAGCTCAA GGACACGGAG
    CTCATCTATG GCGCCAAGCA TGCCTGGCGG AACGCCTCTC GCTGTGTGGG CAGGATCCAG
    TGGTCCAAGC TGCAGGTGTT CGATGCCCGA GACTGCACCA CAGCCCATGG GATGTTCAAC
    TACATCTGTA ACCACGTCAA GTACGCCACC AACAAAGGGA ACCTCAGGTC GGCCATCACT
    ATATTCCCTC AAAGGACTGA TGGCAAGCAT GACTTCCGAG TGTGGAACTC GCAGCTCATC
    CGCTATGCCG GCTACAAGCA GCCAGATGGC TCTACCTTGG GCGATCCAGC TAATGTGGAG
    TTCACAGAGA TCTGTATACA GCAGGGCTGG AAACCCCCAA GAGGCCGGTT CGATGTGCTG
    CCTCTCCTGC TCCAGGCCAA TGGCAATGAC CCTGAACTCT TTCAGATCCC CCCAGAGCTG
    GTGCTGGAAG TACCCATCAG GCACCCCAAG TTCGACTGGT TTAAGGACCT GGGGCTCAAA
    TGGTATGGCC TCCCCGCTGT GTCCAACATG CTGCTGGAGA TTGGGGGCCT GGAGTTCAGC
    GCCTGTCCCT TTAGTGGCTG GTACATGGGC ACGGAGATTG GCGTTCGTGA TTACTGTGAC
    AACTCTCGAT ACAACATCCT GGAGGAAGTA GCCAAGAAAA TGGATTTGGA CATGAGGAAG
    ACATCCTCCC TCTGGAAGGA CCAAGCCCTG GTGGAGATTA ACATTGCTGT CCTATACAGC
    TTCCAGAGTG ACAAGGTGAC CATTGTGGAC CACCACTCTG CCACCGAGTC CTTCATCAAA
    CACATGGAGA ATGAGTACCG CTGCAGAGGG GGCTGTCCCG CCGACTGGGT GTGGATTGTG
    CCCCCCATGT CGGGCAGCAT CACCCCTGTC TTCCACCAGG AGATGCTCAA CTACCGGCTC
    ACCCCGTCCT TTGAGTACCA GCCTGATCCA TGGAACACCC ACGTGTGGAA GGGCACCAAT
    GGGACCCCCA CGAAGCGGCG AGCCATTGGC TTCAAGAAAT TGGCAGAGGC CGTCAAGTTC
    TCAGCCAAGC TGATGGGACA GGCCATGGCC AAGAGAGTCA AAGCGACCAT TCTCTACGCC
    ACCGAGACAG GCAAATCCCA AGCCTATGCC AAGACCCTGT GCGAGATCTT CAAGCATGCC
    TTCGATGCCA AGGCTATGTC CATGGAGGAA TATGACATCG TGCACCTGGA GCACGAAGCC
    CTGGTCTTGG TGGTCACCAG CACCTTTGGC AATGGAGACC CCCCAGAGAA TGGGGAGAAA
    TTCGGCTGTG CTTTGATGGA GATGAGGCAC CCCAACTCTG TGCAAGAGGA GAGGAAGTAC
    CCGGAACCCT TGCGTTTCTT TCCCCGTAAA GGGCCTTCCC TCTCCCATGC TGACTCTGAA
    GCCCACAGTC TGGTTGCTGC CCGTGACAGC CAGCACAGGA GCTACAAGGT CCGATTCAAC
    AGCGTCTCCT CCTATTCTGA CTCCCGCAAG TCATCAGGCG ATGGACCAGA CCTCAGAGAC
    AACTTTGAAA GTACTGGACC GCTGGCCAAT GTGAGGTTCT CAGTCTTCGG CCTGGGCTCT
    CGGGCATACC CTCACTTCTG TGCCTTTGGG CACGCAGTGG ACACTCTCCT GGAGGAGCTG
    GGCGGGGAGA GGATTCTGAA GATGAGGGAG GGAGATGAGC TCTGCGGACA GGAAGAGGCT
    TTCAGGACCT GGGCTAAGAA AGTCTTCAAG GCAGCCTGTG ATGTGTTCTG TGTGGGGGAT
    GACGTCAACA TTGAGAAGGC GAACAATTCC CTCATTAGCA ATGACCGCAG CTGGAAGAGG
    AATAAATTCC GCCTCACTTA TGTGGCAGAA GCTCCAGAGC TGACCCAAGG TCTTTCCAAT
    GTTCACAAAA AGCGAGTCTC AGCCGCCCGA CTCCTCAGCC GCCAAAACCT GCAAAGTCCT
    AAATCCAGCC GATCGACCAT CTTCGTGCGT CTCCACACCA ACGGGAATCA GGAGTTGCAG
    TACCAGCCAG GGGACCACCT GGGTGTCTTC CCTGGCAACC ATGAGGACCT CGTGAATGCA
    CTCATTGAAC GGCTGGAGGA CGCGCCGCCT GCCAACCACG TGGTGAAGGT GGAAATGCTG
    GAGGAAAGGA ACACGGCTCT GGGTGTCATC AGTAATTGGA AGGATGAATC TCGCCTCCCA
    CCCTGCACCA TCTTCCAGGC CTTCAAGTAC TACCTGGACA TCACCACGCC GCCCACGCCC
    CTGCAGCTGC AGCAGTTCGC CTCCCTGGCC ACCAATGAGA AAGAGAAGCA GCGGCTGCTG
    GTCCTCAGCA AGGGGCTTCA GGAATATGAG GAATGGAAAT GGGGCAAGAA CCCCACAATG
    GTGGAGGTGC TGGAGGAGTT CCCGTCCATC CAGATGCCGG CCACACTTCT CCTCACACAG
    CTGTCGCTGC TACAACCTCG CTACTATTCC ATCAGCTCCT CTCCAGACAT GTACCCCGAC
    GAGGTGCACC TCACCGTGGC CATCGTGTCC TACCATACCC GAGATGGAGA AGGACCGGTT
    CACCACGGGG TGTGCTCCTC CTGGCTCAAC CGAATACAGG CTGACGATGT CGTCCCCTGC
    TTCGTGAGAG GTGCCCCTAG CTTCCACCTG CCTCGAAACC CCCAGGTGCC TTGCATCCTG
    GTTGGCCCAG GCACCGGCAT CGCTCCCTTC CGAAGTTTTT GGCAACAGCG ACAATTTGAC
    ATCCAACACA AAGGAATGAA TCCGTGCCCC ATGGTTCTGG TCTTCGGGTG TCGACAATCC
    AAGATAGATC ATATCTACAG AGAGGAGACC CTGCAGGCCA AGAACAAGGG CGTCTTCAGA
    GAGCTGTACA CTGCCTACTC CCGGGAACCC GACAGGCCAA AGAAATATGT ACAGGACGTG
    CTGCAGGAAC AGCTGGCCGA GTCTGTGTAC CGCGCCCTGA AGGAGCAAGG AGGCCATATT
    TATGTCTGCG GGGACGTTAC CATGGCCGCT GATGTCCTCA AAGCCATCCA GCGCATAATG
    ACCCAGCAGG GGAAACTCTC GGAGGAGGAT GCTGGTGTGT TCATCAGCAG GCTGAGGGAT
    GACAACCGGT ACCACGAGGA CATCTTTGGG GTCACCCTCA GAACATATGA AGTGACCAAC
    CGCCTTAGAT CTGAGTCCAT CGCCTTCATT GAAGAGAGCA AAAAAGACAC AGATGAGGTT
    TTCAGCTCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu35813
    Clone ID Related Accession (Same CDS sequence) XM_006530192.3
    Accession Version XM_006530192.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4293bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product nitric oxide synthase, brain isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006530192.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAAGAGC ACACGTTTGG GGTCCAGCAG ATCCAACCCA ACGTCATTTC TGTCCGTCTC 
    TTCAAACGCA AAGTGGGAGG TCTGGGCTTC CTGGTGAAGG AACGGGTCAG CAAGCCCCCG
    GTGATCATCT CAGATCTGAT CCGAGGAGGT GCCGCGGAGC AGAGCGGCCT TATCCAAGCC
    GGCGACATCA TTCTCGCCGT CAATGATCGG CCCCTGGTAG ACCTCAGCTA TGACAGCGCT
    CTGGAAGTTC TCAGGGGCAT TGCCTCTGAG ACCCATGTGG TCCTCATTCT GAGAGGCCCT
    GAGGGCTTTA CTACACACCT GGAGACCACC TTCACAGGGG ATGGAACCCC CAAGACCATC
    CGTGTGACCC AGCCCCTGGG GACACCCACC AAAGCTGTCG ATCTGTCTCG CCAGCCATCA
    GCCAGCAAAG ACCAGCCATT AGCAGTAGAC AGGGTCCCAG GTCCCAGTAA CGGACCTCAG
    CATGCCCAAG GCCGTGGACA GGGAGCCGGC TCAGTCTCCC AGGCTAATGG TGTGGCCATT
    GACCCCACAA TGAAAAATAC CAAGGCCAAC CTCCAGGACA GCGGAGAACA GGATGAACTG
    CTCAAAGAGA TAGAACCTGT GTTGAGCATC CTCACCGGTG GGGGAAAAGC AGTCAACAGA
    GGGGGACCAG CCAAAGCAGA GATGAAAGAC ACAGGAATCC AGGTGGACAG AGACCTCGAC
    GGCAAACTGC ACAAAGCTCC GCCCCTGGGC GGGGAGAATG ATCGAGTCTT CAATGATCTG
    TGGGGGAAGG GCAACGTCCC TGTGGTCCTC AACAACCCGT ATTCAGAGAA CGAACAGTCT
    CCCGCCTCGG GCAAACAGTC TCCTACCAAG AATGGCAGTC CTTCCAGGTG CCCTCGCTTC
    CTTAAGGTCA AGAACTGGGA GACAGATGTG GTCCTCACTG ACACCCTGCA CCTGAAGAGC
    ACGCTGGAAA CGGGGTGCAC AGAGCAGATC TGCATGGGCT CCATCATGCT GCCATCCCAT
    CACATACGGA AGTCAGAAGA TGTCCGCACC AAGGACCAGC TCTTCCCTCT AGCCAAAGAA
    TTTCTCGACC AATACTACTC CTCCATTAAG AGGTTTGGCT CCAAGGCCCA CATGGACAGG
    CTGGAGGAGG TGAACAAGGA GATCGAGAGC ACCAGCACCT ACCAGCTCAA GGACACGGAG
    CTCATCTATG GCGCCAAGCA TGCCTGGCGG AACGCCTCTC GCTGTGTGGG CAGGATCCAG
    TGGTCCAAGC TGCAGGTGTT CGATGCCCGA GACTGCACCA CAGCCCATGG GATGTTCAAC
    TACATCTGTA ACCACGTCAA GTACGCCACC AACAAAGGGA ACCTCAGGTC GGCCATCACT
    ATATTCCCTC AAAGGACTGA TGGCAAGCAT GACTTCCGAG TGTGGAACTC GCAGCTCATC
    CGCTATGCCG GCTACAAGCA GCCAGATGGC TCTACCTTGG GCGATCCAGC TAATGTGGAG
    TTCACAGAGA TCTGTATACA GCAGGGCTGG AAACCCCCAA GAGGCCGGTT CGATGTGCTG
    CCTCTCCTGC TCCAGGCCAA TGGCAATGAC CCTGAACTCT TTCAGATCCC CCCAGAGCTG
    GTGCTGGAAG TACCCATCAG GCACCCCAAG TTCGACTGGT TTAAGGACCT GGGGCTCAAA
    TGGTATGGCC TCCCCGCTGT GTCCAACATG CTGCTGGAGA TTGGGGGCCT GGAGTTCAGC
    GCCTGTCCCT TTAGTGGCTG GTACATGGGC ACGGAGATTG GCGTTCGTGA TTACTGTGAC
    AACTCTCGAT ACAACATCCT GGAGGAAGTA GCCAAGAAAA TGGATTTGGA CATGAGGAAG
    ACATCCTCCC TCTGGAAGGA CCAAGCCCTG GTGGAGATTA ACATTGCTGT CCTATACAGC
    TTCCAGAGTG ACAAGGTGAC CATTGTGGAC CACCACTCTG CCACCGAGTC CTTCATCAAA
    CACATGGAGA ATGAGTACCG CTGCAGAGGG GGCTGTCCCG CCGACTGGGT GTGGATTGTG
    CCCCCCATGT CGGGCAGCAT CACCCCTGTC TTCCACCAGG AGATGCTCAA CTACCGGCTC
    ACCCCGTCCT TTGAGTACCA GCCTGATCCA TGGAACACCC ACGTGTGGAA GGGCACCAAT
    GGGACCCCCA CGAAGCGGCG AGCCATTGGC TTCAAGAAAT TGGCAGAGGC CGTCAAGTTC
    TCAGCCAAGC TGATGGGACA GGCCATGGCC AAGAGAGTCA AAGCGACCAT TCTCTACGCC
    ACCGAGACAG GCAAATCCCA AGCCTATGCC AAGACCCTGT GCGAGATCTT CAAGCATGCC
    TTCGATGCCA AGGCTATGTC CATGGAGGAA TATGACATCG TGCACCTGGA GCACGAAGCC
    CTGGTCTTGG TGGTCACCAG CACCTTTGGC AATGGAGACC CCCCAGAGAA TGGGGAGAAA
    TTCGGCTGTG CTTTGATGGA GATGAGGCAC CCCAACTCTG TGCAAGAGGA GAGGAAGAGC
    TACAAGGTCC GATTCAACAG CGTCTCCTCC TATTCTGACT CCCGCAAGTC ATCAGGCGAT
    GGACCAGACC TCAGAGACAA CTTTGAAAGT ACTGGACCGC TGGCCAATGT GAGGTTCTCA
    GTCTTCGGCC TGGGCTCTCG GGCATACCCT CACTTCTGTG CCTTTGGGCA CGCAGTGGAC
    ACTCTCCTGG AGGAGCTGGG CGGGGAGAGG ATTCTGAAGA TGAGGGAGGG AGATGAGCTC
    TGCGGACAGG AAGAGGCTTT CAGGACCTGG GCTAAGAAAG TCTTCAAGGC AGCCTGTGAT
    GTGTTCTGTG TGGGGGATGA CGTCAACATT GAGAAGGCGA ACAATTCCCT CATTAGCAAT
    GACCGCAGCT GGAAGAGGAA TAAATTCCGC CTCACTTATG TGGCAGAAGC TCCAGAGCTG
    ACCCAAGGTC TTTCCAATGT TCACAAAAAG CGAGTCTCAG CCGCCCGACT CCTCAGCCGC
    CAAAACCTGC AAAGTCCTAA ATCCAGCCGA TCGACCATCT TCGTGCGTCT CCACACCAAC
    GGGAATCAGG AGTTGCAGTA CCAGCCAGGG GACCACCTGG GTGTCTTCCC TGGCAACCAT
    GAGGACCTCG TGAATGCACT CATTGAACGG CTGGAGGACG CGCCGCCTGC CAACCACGTG
    GTGAAGGTGG AAATGCTGGA GGAAAGGAAC ACGGCTCTGG GTGTCATCAG TAATTGGAAG
    GATGAATCTC GCCTCCCACC CTGCACCATC TTCCAGGCCT TCAAGTACTA CCTGGACATC
    ACCACGCCGC CCACGCCCCT GCAGCTGCAG CAGTTCGCCT CCCTGGCCAC CAATGAGAAA
    GAGAAGCAGC GGCTGCTGGT CCTCAGCAAG GGGCTTCAGG AATATGAGGA ATGGAAATGG
    GGCAAGAACC CCACAATGGT GGAGGTGCTG GAGGAGTTCC CGTCCATCCA GATGCCGGCC
    ACACTTCTCC TCACACAGCT GTCGCTGCTA CAACCTCGCT ACTATTCCAT CAGCTCCTCT
    CCAGACATGT ACCCCGACGA GGTGCACCTC ACCGTGGCCA TCGTGTCCTA CCATACCCGA
    GATGGAGAAG GACCGGTTCA CCACGGGGTG TGCTCCTCCT GGCTCAACCG AATACAGGCT
    GACGATGTCG TCCCCTGCTT CGTGAGAGGT GCCCCTAGCT TCCACCTGCC TCGAAACCCC
    CAGGTGCCTT GCATCCTGGT TGGCCCAGGC ACCGGCATCG CTCCCTTCCG AAGTTTTTGG
    CAACAGCGAC AATTTGACAT CCAACACAAA GGAATGAATC CGTGCCCCAT GGTTCTGGTC
    TTCGGGTGTC GACAATCCAA GATAGATCAT ATCTACAGAG AGGAGACCCT GCAGGCCAAG
    AACAAGGGCG TCTTCAGAGA GCTGTACACT GCCTACTCCC GGGAACCCGA CAGGCCAAAG
    AAATATGTAC AGGACGTGCT GCAGGAACAG CTGGCCGAGT CTGTGTACCG CGCCCTGAAG
    GAGCAAGGAG GCCATATTTA TGTCTGCGGG GACGTTACCA TGGCCGCTGA TGTCCTCAAA
    GCCATCCAGC GCATAATGAC CCAGCAGGGG AAACTCTCGG AGGAGGATGC TGGTGTGTTC
    ATCAGCAGGC TGAGGGATGA CAACCGGTAC CACGAGGACA TCTTTGGGGT CACCCTCAGA
    ACATATGAAG TGACCAACCG CCTTAGATCT GAGTCCATCG CCTTCATTGA AGAGAGCAAA
    AAAGACACAG ATGACAGGGT TTTCAGCTCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006530255.1
    CDS1110..5402
    Translation

    Target ORF information:

    RefSeq Version XM_006530192.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus nitric oxide synthase 1, neuronal (Nos1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006530192.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAAGAGC ACACGTTTGG GGTCCAGCAG ATCCAACCCA ACGTCATTTC TGTCCGTCTC 
    TTCAAACGCA AAGTGGGAGG TCTGGGCTTC CTGGTGAAGG AACGGGTCAG CAAGCCCCCG
    GTGATCATCT CAGATCTGAT CCGAGGAGGT GCCGCGGAGC AGAGCGGCCT TATCCAAGCC
    GGCGACATCA TTCTCGCCGT CAATGATCGG CCCCTGGTAG ACCTCAGCTA TGACAGCGCT
    CTGGAAGTTC TCAGGGGCAT TGCCTCTGAG ACCCATGTGG TCCTCATTCT GAGAGGCCCT
    GAGGGCTTTA CTACACACCT GGAGACCACC TTCACAGGGG ATGGAACCCC CAAGACCATC
    CGTGTGACCC AGCCCCTGGG GACACCCACC AAAGCTGTCG ATCTGTCTCG CCAGCCATCA
    GCCAGCAAAG ACCAGCCATT AGCAGTAGAC AGGGTCCCAG GTCCCAGTAA CGGACCTCAG
    CATGCCCAAG GCCGTGGACA GGGAGCCGGC TCAGTCTCCC AGGCTAATGG TGTGGCCATT
    GACCCCACAA TGAAAAATAC CAAGGCCAAC CTCCAGGACA GCGGAGAACA GGATGAACTG
    CTCAAAGAGA TAGAACCTGT GTTGAGCATC CTCACCGGTG GGGGAAAAGC AGTCAACAGA
    GGGGGACCAG CCAAAGCAGA GATGAAAGAC ACAGGAATCC AGGTGGACAG AGACCTCGAC
    GGCAAACTGC ACAAAGCTCC GCCCCTGGGC GGGGAGAATG ATCGAGTCTT CAATGATCTG
    TGGGGGAAGG GCAACGTCCC TGTGGTCCTC AACAACCCGT ATTCAGAGAA CGAACAGTCT
    CCCGCCTCGG GCAAACAGTC TCCTACCAAG AATGGCAGTC CTTCCAGGTG CCCTCGCTTC
    CTTAAGGTCA AGAACTGGGA GACAGATGTG GTCCTCACTG ACACCCTGCA CCTGAAGAGC
    ACGCTGGAAA CGGGGTGCAC AGAGCAGATC TGCATGGGCT CCATCATGCT GCCATCCCAT
    CACATACGGA AGTCAGAAGA TGTCCGCACC AAGGACCAGC TCTTCCCTCT AGCCAAAGAA
    TTTCTCGACC AATACTACTC CTCCATTAAG AGGTTTGGCT CCAAGGCCCA CATGGACAGG
    CTGGAGGAGG TGAACAAGGA GATCGAGAGC ACCAGCACCT ACCAGCTCAA GGACACGGAG
    CTCATCTATG GCGCCAAGCA TGCCTGGCGG AACGCCTCTC GCTGTGTGGG CAGGATCCAG
    TGGTCCAAGC TGCAGGTGTT CGATGCCCGA GACTGCACCA CAGCCCATGG GATGTTCAAC
    TACATCTGTA ACCACGTCAA GTACGCCACC AACAAAGGGA ACCTCAGGTC GGCCATCACT
    ATATTCCCTC AAAGGACTGA TGGCAAGCAT GACTTCCGAG TGTGGAACTC GCAGCTCATC
    CGCTATGCCG GCTACAAGCA GCCAGATGGC TCTACCTTGG GCGATCCAGC TAATGTGGAG
    TTCACAGAGA TCTGTATACA GCAGGGCTGG AAACCCCCAA GAGGCCGGTT CGATGTGCTG
    CCTCTCCTGC TCCAGGCCAA TGGCAATGAC CCTGAACTCT TTCAGATCCC CCCAGAGCTG
    GTGCTGGAAG TACCCATCAG GCACCCCAAG TTCGACTGGT TTAAGGACCT GGGGCTCAAA
    TGGTATGGCC TCCCCGCTGT GTCCAACATG CTGCTGGAGA TTGGGGGCCT GGAGTTCAGC
    GCCTGTCCCT TTAGTGGCTG GTACATGGGC ACGGAGATTG GCGTTCGTGA TTACTGTGAC
    AACTCTCGAT ACAACATCCT GGAGGAAGTA GCCAAGAAAA TGGATTTGGA CATGAGGAAG
    ACATCCTCCC TCTGGAAGGA CCAAGCCCTG GTGGAGATTA ACATTGCTGT CCTATACAGC
    TTCCAGAGTG ACAAGGTGAC CATTGTGGAC CACCACTCTG CCACCGAGTC CTTCATCAAA
    CACATGGAGA ATGAGTACCG CTGCAGAGGG GGCTGTCCCG CCGACTGGGT GTGGATTGTG
    CCCCCCATGT CGGGCAGCAT CACCCCTGTC TTCCACCAGG AGATGCTCAA CTACCGGCTC
    ACCCCGTCCT TTGAGTACCA GCCTGATCCA TGGAACACCC ACGTGTGGAA GGGCACCAAT
    GGGACCCCCA CGAAGCGGCG AGCCATTGGC TTCAAGAAAT TGGCAGAGGC CGTCAAGTTC
    TCAGCCAAGC TGATGGGACA GGCCATGGCC AAGAGAGTCA AAGCGACCAT TCTCTACGCC
    ACCGAGACAG GCAAATCCCA AGCCTATGCC AAGACCCTGT GCGAGATCTT CAAGCATGCC
    TTCGATGCCA AGGCTATGTC CATGGAGGAA TATGACATCG TGCACCTGGA GCACGAAGCC
    CTGGTCTTGG TGGTCACCAG CACCTTTGGC AATGGAGACC CCCCAGAGAA TGGGGAGAAA
    TTCGGCTGTG CTTTGATGGA GATGAGGCAC CCCAACTCTG TGCAAGAGGA GAGGAAGAGC
    TACAAGGTCC GATTCAACAG CGTCTCCTCC TATTCTGACT CCCGCAAGTC ATCAGGCGAT
    GGACCAGACC TCAGAGACAA CTTTGAAAGT ACTGGACCGC TGGCCAATGT GAGGTTCTCA
    GTCTTCGGCC TGGGCTCTCG GGCATACCCT CACTTCTGTG CCTTTGGGCA CGCAGTGGAC
    ACTCTCCTGG AGGAGCTGGG CGGGGAGAGG ATTCTGAAGA TGAGGGAGGG AGATGAGCTC
    TGCGGACAGG AAGAGGCTTT CAGGACCTGG GCTAAGAAAG TCTTCAAGGC AGCCTGTGAT
    GTGTTCTGTG TGGGGGATGA CGTCAACATT GAGAAGGCGA ACAATTCCCT CATTAGCAAT
    GACCGCAGCT GGAAGAGGAA TAAATTCCGC CTCACTTATG TGGCAGAAGC TCCAGAGCTG
    ACCCAAGGTC TTTCCAATGT TCACAAAAAG CGAGTCTCAG CCGCCCGACT CCTCAGCCGC
    CAAAACCTGC AAAGTCCTAA ATCCAGCCGA TCGACCATCT TCGTGCGTCT CCACACCAAC
    GGGAATCAGG AGTTGCAGTA CCAGCCAGGG GACCACCTGG GTGTCTTCCC TGGCAACCAT
    GAGGACCTCG TGAATGCACT CATTGAACGG CTGGAGGACG CGCCGCCTGC CAACCACGTG
    GTGAAGGTGG AAATGCTGGA GGAAAGGAAC ACGGCTCTGG GTGTCATCAG TAATTGGAAG
    GATGAATCTC GCCTCCCACC CTGCACCATC TTCCAGGCCT TCAAGTACTA CCTGGACATC
    ACCACGCCGC CCACGCCCCT GCAGCTGCAG CAGTTCGCCT CCCTGGCCAC CAATGAGAAA
    GAGAAGCAGC GGCTGCTGGT CCTCAGCAAG GGGCTTCAGG AATATGAGGA ATGGAAATGG
    GGCAAGAACC CCACAATGGT GGAGGTGCTG GAGGAGTTCC CGTCCATCCA GATGCCGGCC
    ACACTTCTCC TCACACAGCT GTCGCTGCTA CAACCTCGCT ACTATTCCAT CAGCTCCTCT
    CCAGACATGT ACCCCGACGA GGTGCACCTC ACCGTGGCCA TCGTGTCCTA CCATACCCGA
    GATGGAGAAG GACCGGTTCA CCACGGGGTG TGCTCCTCCT GGCTCAACCG AATACAGGCT
    GACGATGTCG TCCCCTGCTT CGTGAGAGGT GCCCCTAGCT TCCACCTGCC TCGAAACCCC
    CAGGTGCCTT GCATCCTGGT TGGCCCAGGC ACCGGCATCG CTCCCTTCCG AAGTTTTTGG
    CAACAGCGAC AATTTGACAT CCAACACAAA GGAATGAATC CGTGCCCCAT GGTTCTGGTC
    TTCGGGTGTC GACAATCCAA GATAGATCAT ATCTACAGAG AGGAGACCCT GCAGGCCAAG
    AACAAGGGCG TCTTCAGAGA GCTGTACACT GCCTACTCCC GGGAACCCGA CAGGCCAAAG
    AAATATGTAC AGGACGTGCT GCAGGAACAG CTGGCCGAGT CTGTGTACCG CGCCCTGAAG
    GAGCAAGGAG GCCATATTTA TGTCTGCGGG GACGTTACCA TGGCCGCTGA TGTCCTCAAA
    GCCATCCAGC GCATAATGAC CCAGCAGGGG AAACTCTCGG AGGAGGATGC TGGTGTGTTC
    ATCAGCAGGC TGAGGGATGA CAACCGGTAC CACGAGGACA TCTTTGGGGT CACCCTCAGA
    ACATATGAAG TGACCAACCG CCTTAGATCT GAGTCCATCG CCTTCATTGA AGAGAGCAAA
    AAAGACACAG ATGACAGGGT TTTCAGCTCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.