nos1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	nos1
Entrez Gene ID	100497595
Full Name	nitric oxide synthase 1
Synonyms	bNOS,ihps1,nnos,nos
General protein information	Preferred Names nitric oxide synthase 1 Names nitric oxide synthase, brain neural nitric oxide synthase nitric oxide synthase 1 (neuronal)
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031892799.1	XP_031748659.1	nitric oxide synthase, brain isoform X1
XM_031892791.1	XP_031748651.1	nitric oxide synthase, brain isoform X1
XM_031892798.1	XP_031748658.1	nitric oxide synthase, brain isoform X1
XM_012968764.2	XP_012824218.1	nitric oxide synthase, brain isoform X2
XM_004910501.3	XP_004910558.1	nitric oxide synthase, brain isoform X1
XM_031892807.1	XP_031748667.1	nitric oxide synthase, brain isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NOS1	XP_003314004.2
Rattus norvegicus (Norway rat)	Nos1	NP_434686.1
Gallus gallus (chicken)	NOS1	XP_004934537.1
Xenopus tropicalis (tropical clawed frog)	nos1	XP_004910558.1
	NOS1	XP_001083352.2
Mus musculus (house mouse)	Nos1	NP_032738.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008255	XP_317213.1
Homo sapiens (human)	NOS1	NP_000611.1
Bos taurus (cattle)	NOS1	XP_002694631.3
Canis lupus familiaris (dog)	NOS1	NP_001182074.1
Danio rerio (zebrafish)	nos1	NP_571735.1
Drosophila melanogaster (fruit fly)	Nos	NP_523541.2

Gene Ontology

The following nos1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nos1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa03509	XM_031892799.1 Latest version!	Xenopus tropicalis nitric oxide synthase 1 (nos1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa03509	XM_031892807.1 Latest version!	Xenopus tropicalis nitric oxide synthase 1 (nos1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa03509	XM_031892798.1 Latest version!	Xenopus tropicalis nitric oxide synthase 1 (nos1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa03509	XM_031892791.1 Latest version!	Xenopus tropicalis nitric oxide synthase 1 (nos1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa03509	XM_004910501.3 Latest version!	Xenopus tropicalis nitric oxide synthase 1 (nos1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa03510	XM_012968764.2 Latest version!	Xenopus tropicalis nitric oxide synthase 1 (nos1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nos1 ( XM_031892799.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031748659.1 Xenopus tropicalis nitric oxide synthase 1 (nos1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa03509
Clone ID Related Accession (Same CDS sequence)	XM_031892799.1 , XM_031892791.1 , XM_031892798.1 , XM_004910501.3 , XM_031892807.1
Accession Version	XM_031892799.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4263bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nitric oxide synthase, brain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAAT ATGAATTCAG CGTTAAACAA CTGCAGCCGA ATGTTATATC AGTACGCCTC 
TTTAAACGCA AAGTGGGCGG GCTGGGATTC CTGGTTAAGC AAAGGAGGAA CAAACCACCT 
GTAATTATTT CTGATTTAAT TCGGGGAGGG GCAGCAGAGC AGAGTGGGTT GGTGCAAGTA 
GGGGATATTA TCTTAGCAGT GAATGATAGG CCCCTTGTAG ATGCCAGCTA TGAAAGTGCC 
CTGGAGATAC TGCGTAGCAT TTCCACAGAG ACTTTCGTTG TACTGATCCT TAGAGGTCCA 
GAAGGATTCA CAACCCACTT GGAGACCACC TTTTCTGGAG ATGGGACTCC CAAGACAATT 
CGGGTCACCA GACCTCTGTG TTCTAAGTCC AAGTCAACTG ACTTGACAAG TCAGAGCCTT 
TATAGCAAAG ATCACCAAAA TATGGTGGAC AGTGCCACAA GTTCAGCTGG CTCTTCATGG 
CAGGAGGTTC ATCAGCTTCT TAACTTAAAT GGACTTGATG GTGGTAAAGG TGATGACATC 
AGTATCAGCC CTTGCCTTAA TGGAGGAGAA AAAGCTAATG ACATATTGAA AGAAATTGAG 
CCAGTTGTGA GCCTTCTGAA TGCAGGAATG GACCTAAATG GGGATGGACA TCGAAACGTG 
AAGCAAAAGG ATGCGGAAGT TCAGGTTGAA AGCAACTCGC AGACACAACC AAGCACGCAG 
AAGGACCAAG TCAATGGTAT CTGGAAAAAC AACAACAAGA TGCCTGTGGT TTTAAACAAC 
CCATATCTGG AGTGTGAGCA GGTGACATTA ACTGGTAGAC ATTCCCCTGC AAAGAGCCAA 
GTGAATGGTA GTCCCTCCAA ATGTCCTCGT TATATGAAAA TTCGTAACTG GGAGACAAAC 
ACTTCACTTA ATGACACGCT GCACTGCAAG GCTTCCATGC CAACACCCTG CACACAACAG 
ATATGCATGG GGGCAGTGAT GACGCCACCA CATTATGTTC GCAAACCAGA GGACGTCCGG 
ACCAAGGAGG AGCTTCTTCC CCTTGCTAAG GAATTCATTG ACCAATATTA CTCTTCAATG 
AAAAGATTTG GCTCCAAGGC ACACATGGAC AGACTACAAG AAGTAACAAA GGAGATTGAA 
GCTACAGATA CTTATCAGCT ACGAGATACA GAGCTTATTT ATGGAGCCAA GCATGCCTGG 
AGGAATGCAT CCCGATGTGT GGGGAGAATC CAGTGGTCAA AACTGCAGGT ATTTGATGCT 
CGAGACTGCA CCACTGCTCA TGGGATGTTC AATTACATTT GCAATCACAT TAAATATGCC 
ACCAACAAAG GCAACCTCAG ATCAGCAATC ACTATCTTCC CTCAAAGAAC GGATGGGAAG 
CGTGACTTTA GAATCTGGAA TGCTCAGCTT ATTCGCTATG CAGGATACAA ACAGCCTGAT 
GGCTCAGTGC TGGGAGACCC TGCAAATGTA GAATTGACAG AGATCTGCAT TCAGCAGGGC 
TGGAAGGCCC CTTATGGACG TTTTGATGTA CTACCTCTAC TGCTCCAAGC TGATGGCAAT 
GACCCTGAAT TGTTTGAGAT CCCCCCTGAA CTGATCATGG AAGTCAACAT TAGGCACCCC 
AGGCTAGAGT GGTTTAAAGA CCTGGCTTTG AAGTGGTATG GGCTGCCTGC TGTATCCAAT 
TTGCTGCTAG AAGTTGGAGG CTTGGAATTC AGTGCCTGTC CCTTCAGCGG ATGGTACATG 
GGGACAGAAA TTGGTGTTCG AGATTACTGT GACAACTCCC GCTACAACAT ACTGGAGGAA 
GTAGCCCTGA AAATGAATCT GGACACAAGG AAAACATCCT CTTTGTGGAA GGATCAAGCT 
CTGGTGGAGA TAAACATTGC TGTATTGTAC AGTTTCCAGA GTGATAAAAT AACAATCGTC 
GACCATCACT CAGCTACTGA GTCTTTCATC AAACATATGG AGAATGAATA CCGCTGCCGA 
GGAGGCTGTC CTGCAGACTG GGTTTGGATA GTACCGCCGA TGTCTGGAAG CATTACCCCT 
GTATTTCACC AGGAAATGTT AAACTACCGT CTCACACCTT CATTTGAGTA CCAGCCTGAT 
CCATGGAATA CCCACATATG GAAAGGGGTT AATGGAACAC CAACAAAGAA AAGAGCCATT 
GGATTTAAAA AGCTGGCAAA GGCAGTTAAA TTTTCAGCTA AATTAATGGG ACAGGCCATG 
GCTAAAAGAG TAAAGGCTAC AATTCTCTAC GCAACAGAAA CTGGGAAATC CGAAGTCTAT 
GCAAAAACTT TATGTGAAAT CTTCAAGCAT GCATTTGATG CAAAGGTTAT GTCAATGGAT 
GACTATGACA TTGTGCACCT AGAGCATGAA ACACTTGTCT TAGTAGTGAC TAGTACTTTT 
GGTAATGGTG ATCCACCAGA AAATGGGGAG AAGTTTGGCT GTGCATTAAT GGAAATGAGG 
CATCCCAACT CCAACCTGGA GGAGAGGACA AGCTACAAAG TCCGTTTTAA CAGCGTCTCA 
TCCTCTGCTG ATGCCCAGAA ATCGTCTGCT GATGAGACAG GACCCAGAGA CAACTTTGGT 
AGTGCAGGTC CCCTAGCTAA TGTTAGATTC TCAGCGTTTG GACTTGGCTC CCGAGCTTAC 
CCCCACTTCT GTGCCTTTGC CCGTGCGGTT GACACCCTGC TGGAGGAGCT TGGAGGAGAA 
AGAATACTGA AAATGGGGGA AGGGGATGAA TTGTGCGGTC AGGAAGAATC CTTTAGAACA 
TGGGCCAAAA AAGTGTTTCA GGCTGCATGT GACGTCTTTT GTGTAGGAGA TGATGTAAGT 
ATTGAAAAAG CAAACAACTC ACTGATTAGC AATGACCGCA GCTGGAAAAG AAGCAAGTAC 
CGGATCAGCT ATGTGGCAGA GGCACCAGAA CTTACACAAG CATTATACAG TATTCACAAG 
AAGAAAGTGT ACGGAGCTCG ATTGTTGTCT CGCCAGAACT TGCAAAGCCC AAAATCCAGC 
CGATCCACTA TATTCCTTCA ACTTCACACT AACGAACATA AAGAACTGTG CTACAAACCT 
GGAGACCATT TGGGAATCTT TCCTGGAAAC CATGAAGATC TAGTGAATGC CTTGATAGAG 
CAATTAGAAG ATGCACCTCC TGTAAACCAG CTGGTGAGGG TGGAGATGCT GGAAGAAAGA 
AATACTGCTT TAGGTGTAAT TAGTAACTGG ACAGAGGAGC AGCGAATCCC ACCCTGCACC 
ATCTTTCAAG CATTTAAGTA TTTCTTAGAT ATCACCACAC CCCCTACCCC TCTCCTCATG 
CAGCAAATTG CGCTGCTGGC AACTGATGAG AAAGAAAAGA ATCGGCTGGA GATCTTAAGC 
AAGGGCTTGC AGGAATATGA GCAATGGAAG TGGTACAAGA ATCCTACTAT TGTTGAGGTG 
CTAGAAGAGT TTCCTTCAAT TCAGATGCCT TCATCCCTGC TGCTTACACA ACTACCCTTG 
CTACAGCCTC GATACTACTC CATCAGCTCA TCACCTGAGA TGTATCCGGA AGAAGTGCAC 
CTTACTGTTG CTGTTGTGAC CTACCGGACA AGAGATGGAG ATGGGCCTAT ACATCATGGA 
GTCTGCTCCT CATGGCTCAA CAGAATACAG CCAGATGAAG TTGTGCCCTG CTTTGTCAGA 
GGAGCACCAA GTTTCCAGAT GCCAGAAGAC CCACAGGTCC CCTGCATTCT GATAGGACCA 
GGCACTGGCA TTGCTCCATT TAGGAGTTTT TGGCAGCAAC GTCTCTATGA TATGCAGCAC 
AGAGGACTAA AACCTTGTCC AATGATCTTG GTATTTGGGT GTAGGGAATC CAAAATTGAC 
CACATCTACA AAGAGGAAAC CATGCATGCA ATGAATAAAG GAGTGTTCAA AGAACTCTTT 
ACAGCTTATT CTCGGGAACC TAATAAACCA AAGAAATACG TTCAGGATGT ACTGAGGGAA 
CAGTTATCTG ATGTGACATA CAAAGCTCTG AAGGATCAAG GGGGACACAT ATATATTTGT 
GGTGATGTCA ACATGGCTGG GGATGTCCTG AAGAGTCTTC AGCATATTGT GAAAGAGCGT 
GGAAATCTGA CAGCTGAAGA AGCTGGGGCC TTCATCAGCA AACTAAGAGA TGACAATCGG 
TACCATGAAG ACATTTTTGG AGTCACTCTT AGGACGTATG AAGTTACAAA TCGTCTGAGA 
TCTGAATCTA TTGCATTTAT TGAGGAAAGC AAAAAGGACT CAGATGAGGT TTTCTGCTCA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031748659.1
CDS	435..4697
Translation	MEEYEFSVKQLQPNVISVRLFKRKVGGLGFLVKQRRNKPPVIISDLIRGGAAEQSGLVQVGDIILAVNDRPLVDASYESALEILRSISTETFVVLILRGPEGFTTHLETTFSGDGTPKTIRVTRPLCSKSKSTDLTSQSLYSKDHQNMVDSATSSAGSSWQEVHQLLNLNGLDGGKGDDISISPCLNGGEKANDILKEIEPVVSLLNAGMDLNGDGHRNVKQKDAEVQVESNSQTQPSTQKDQVNGIWKNNNKMPVVLNNPYLECEQVTLTGRHSPAKSQVNGSPSKCPRYMKIRNWETNTSLNDTLHCKASMPTPCTQQICMGAVMTPPHYVRKPEDVRTKEELLPLAKEFIDQYYSSMKRFGSKAHMDRLQEVTKEIEATDTYQLRDTELIYGAKHAWRNASRCVGRIQWSKLQVFDARDCTTAHGMFNYICNHIKYATNKGNLRSAITIFPQRTDGKRDFRIWNAQLIRYAGYKQPDGSVLGDPANVELTEICIQQGWKAPYGRFDVLPLLLQADGNDPELFEIPPELIMEVNIRHPRLEWFKDLALKWYGLPAVSNLLLEVGGLEFSACPFSGWYMGTEIGVRDYCDNSRYNILEEVALKMNLDTRKTSSLWKDQALVEINIAVLYSFQSDKITIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMLNYRLTPSFEYQPDPWNTHIWKGVNGTPTKKRAIGFKKLAKAVKFSAKLMGQAMAKRVKATILYATETGKSEVYAKTLCEIFKHAFDAKVMSMDDYDIVHLEHETLVLVVTSTFGNGDPPENGEKFGCALMEMRHPNSNLEERTSYKVRFNSVSSSADAQKSSADETGPRDNFGSAGPLANVRFSAFGLGSRAYPHFCAFARAVDTLLEELGGERILKMGEGDELCGQEESFRTWAKKVFQAACDVFCVGDDVSIEKANNSLISNDRSWKRSKYRISYVAEAPELTQALYSIHKKKVYGARLLSRQNLQSPKSSRSTIFLQLHTNEHKELCYKPGDHLGIFPGNHEDLVNALIEQLEDAPPVNQLVRVEMLEERNTALGVISNWTEEQRIPPCTIFQAFKYFLDITTPPTPLLMQQIALLATDEKEKNRLEILSKGLQEYEQWKWYKNPTIVEVLEEFPSIQMPSSLLLTQLPLLQPRYYSISSSPEMYPEEVHLTVAVVTYRTRDGDGPIHHGVCSSWLNRIQPDEVVPCFVRGAPSFQMPEDPQVPCILIGPGTGIAPFRSFWQQRLYDMQHRGLKPCPMILVFGCRESKIDHIYKEETMHAMNKGVFKELFTAYSREPNKPKKYVQDVLREQLSDVTYKALKDQGGHIYICGDVNMAGDVLKSLQHIVKERGNLTAEEAGAFISKLRDDNRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDSDEVFCS

Target ORF information:

RefSeq Version	XM_031892799.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nitric oxide synthase 1 (nos1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031892799.1

ORF Insert Sequence:

ATGGAGGAAT ATGAATTCAG CGTTAAACAA CTGCAGCCGA ATGTTATATC AGTACGCCTC 
TTTAAACGCA AAGTGGGCGG GCTGGGATTC CTGGTTAAGC AAAGGAGGAA CAAACCACCT 
GTAATTATTT CTGATTTAAT TCGGGGAGGG GCAGCAGAGC AGAGTGGGTT GGTGCAAGTA 
GGGGATATTA TCTTAGCAGT GAATGATAGG CCCCTTGTAG ATGCCAGCTA TGAAAGTGCC 
CTGGAGATAC TGCGTAGCAT TTCCACAGAG ACTTTCGTTG TACTGATCCT TAGAGGTCCA 
GAAGGATTCA CAACCCACTT GGAGACCACC TTTTCTGGAG ATGGGACTCC CAAGACAATT 
CGGGTCACCA GACCTCTGTG TTCTAAGTCC AAGTCAACTG ACTTGACAAG TCAGAGCCTT 
TATAGCAAAG ATCACCAAAA TATGGTGGAC AGTGCCACAA GTTCAGCTGG CTCTTCATGG 
CAGGAGGTTC ATCAGCTTCT TAACTTAAAT GGACTTGATG GTGGTAAAGG TGATGACATC 
AGTATCAGCC CTTGCCTTAA TGGAGGAGAA AAAGCTAATG ACATATTGAA AGAAATTGAG 
CCAGTTGTGA GCCTTCTGAA TGCAGGAATG GACCTAAATG GGGATGGACA TCGAAACGTG 
AAGCAAAAGG ATGCGGAAGT TCAGGTTGAA AGCAACTCGC AGACACAACC AAGCACGCAG 
AAGGACCAAG TCAATGGTAT CTGGAAAAAC AACAACAAGA TGCCTGTGGT TTTAAACAAC 
CCATATCTGG AGTGTGAGCA GGTGACATTA ACTGGTAGAC ATTCCCCTGC AAAGAGCCAA 
GTGAATGGTA GTCCCTCCAA ATGTCCTCGT TATATGAAAA TTCGTAACTG GGAGACAAAC 
ACTTCACTTA ATGACACGCT GCACTGCAAG GCTTCCATGC CAACACCCTG CACACAACAG 
ATATGCATGG GGGCAGTGAT GACGCCACCA CATTATGTTC GCAAACCAGA GGACGTCCGG 
ACCAAGGAGG AGCTTCTTCC CCTTGCTAAG GAATTCATTG ACCAATATTA CTCTTCAATG 
AAAAGATTTG GCTCCAAGGC ACACATGGAC AGACTACAAG AAGTAACAAA GGAGATTGAA 
GCTACAGATA CTTATCAGCT ACGAGATACA GAGCTTATTT ATGGAGCCAA GCATGCCTGG 
AGGAATGCAT CCCGATGTGT GGGGAGAATC CAGTGGTCAA AACTGCAGGT ATTTGATGCT 
CGAGACTGCA CCACTGCTCA TGGGATGTTC AATTACATTT GCAATCACAT TAAATATGCC 
ACCAACAAAG GCAACCTCAG ATCAGCAATC ACTATCTTCC CTCAAAGAAC GGATGGGAAG 
CGTGACTTTA GAATCTGGAA TGCTCAGCTT ATTCGCTATG CAGGATACAA ACAGCCTGAT 
GGCTCAGTGC TGGGAGACCC TGCAAATGTA GAATTGACAG AGATCTGCAT TCAGCAGGGC 
TGGAAGGCCC CTTATGGACG TTTTGATGTA CTACCTCTAC TGCTCCAAGC TGATGGCAAT 
GACCCTGAAT TGTTTGAGAT CCCCCCTGAA CTGATCATGG AAGTCAACAT TAGGCACCCC 
AGGCTAGAGT GGTTTAAAGA CCTGGCTTTG AAGTGGTATG GGCTGCCTGC TGTATCCAAT 
TTGCTGCTAG AAGTTGGAGG CTTGGAATTC AGTGCCTGTC CCTTCAGCGG ATGGTACATG 
GGGACAGAAA TTGGTGTTCG AGATTACTGT GACAACTCCC GCTACAACAT ACTGGAGGAA 
GTAGCCCTGA AAATGAATCT GGACACAAGG AAAACATCCT CTTTGTGGAA GGATCAAGCT 
CTGGTGGAGA TAAACATTGC TGTATTGTAC AGTTTCCAGA GTGATAAAAT AACAATCGTC 
GACCATCACT CAGCTACTGA GTCTTTCATC AAACATATGG AGAATGAATA CCGCTGCCGA 
GGAGGCTGTC CTGCAGACTG GGTTTGGATA GTACCGCCGA TGTCTGGAAG CATTACCCCT 
GTATTTCACC AGGAAATGTT AAACTACCGT CTCACACCTT CATTTGAGTA CCAGCCTGAT 
CCATGGAATA CCCACATATG GAAAGGGGTT AATGGAACAC CAACAAAGAA AAGAGCCATT 
GGATTTAAAA AGCTGGCAAA GGCAGTTAAA TTTTCAGCTA AATTAATGGG ACAGGCCATG 
GCTAAAAGAG TAAAGGCTAC AATTCTCTAC GCAACAGAAA CTGGGAAATC CGAAGTCTAT 
GCAAAAACTT TATGTGAAAT CTTCAAGCAT GCATTTGATG CAAAGGTTAT GTCAATGGAT 
GACTATGACA TTGTGCACCT AGAGCATGAA ACACTTGTCT TAGTAGTGAC TAGTACTTTT 
GGTAATGGTG ATCCACCAGA AAATGGGGAG AAGTTTGGCT GTGCATTAAT GGAAATGAGG 
CATCCCAACT CCAACCTGGA GGAGAGGACA AGCTACAAAG TCCGTTTTAA CAGCGTCTCA 
TCCTCTGCTG ATGCCCAGAA ATCGTCTGCT GATGAGACAG GACCCAGAGA CAACTTTGGT 
AGTGCAGGTC CCCTAGCTAA TGTTAGATTC TCAGCGTTTG GACTTGGCTC CCGAGCTTAC 
CCCCACTTCT GTGCCTTTGC CCGTGCGGTT GACACCCTGC TGGAGGAGCT TGGAGGAGAA 
AGAATACTGA AAATGGGGGA AGGGGATGAA TTGTGCGGTC AGGAAGAATC CTTTAGAACA 
TGGGCCAAAA AAGTGTTTCA GGCTGCATGT GACGTCTTTT GTGTAGGAGA TGATGTAAGT 
ATTGAAAAAG CAAACAACTC ACTGATTAGC AATGACCGCA GCTGGAAAAG AAGCAAGTAC 
CGGATCAGCT ATGTGGCAGA GGCACCAGAA CTTACACAAG CATTATACAG TATTCACAAG 
AAGAAAGTGT ACGGAGCTCG ATTGTTGTCT CGCCAGAACT TGCAAAGCCC AAAATCCAGC 
CGATCCACTA TATTCCTTCA ACTTCACACT AACGAACATA AAGAACTGTG CTACAAACCT 
GGAGACCATT TGGGAATCTT TCCTGGAAAC CATGAAGATC TAGTGAATGC CTTGATAGAG 
CAATTAGAAG ATGCACCTCC TGTAAACCAG CTGGTGAGGG TGGAGATGCT GGAAGAAAGA 
AATACTGCTT TAGGTGTAAT TAGTAACTGG ACAGAGGAGC AGCGAATCCC ACCCTGCACC 
ATCTTTCAAG CATTTAAGTA TTTCTTAGAT ATCACCACAC CCCCTACCCC TCTCCTCATG 
CAGCAAATTG CGCTGCTGGC AACTGATGAG AAAGAAAAGA ATCGGCTGGA GATCTTAAGC 
AAGGGCTTGC AGGAATATGA GCAATGGAAG TGGTACAAGA ATCCTACTAT TGTTGAGGTG 
CTAGAAGAGT TTCCTTCAAT TCAGATGCCT TCATCCCTGC TGCTTACACA ACTACCCTTG 
CTACAGCCTC GATACTACTC CATCAGCTCA TCACCTGAGA TGTATCCGGA AGAAGTGCAC 
CTTACTGTTG CTGTTGTGAC CTACCGGACA AGAGATGGAG ATGGGCCTAT ACATCATGGA 
GTCTGCTCCT CATGGCTCAA CAGAATACAG CCAGATGAAG TTGTGCCCTG CTTTGTCAGA 
GGAGCACCAA GTTTCCAGAT GCCAGAAGAC CCACAGGTCC CCTGCATTCT GATAGGACCA 
GGCACTGGCA TTGCTCCATT TAGGAGTTTT TGGCAGCAAC GTCTCTATGA TATGCAGCAC 
AGAGGACTAA AACCTTGTCC AATGATCTTG GTATTTGGGT GTAGGGAATC CAAAATTGAC 
CACATCTACA AAGAGGAAAC CATGCATGCA ATGAATAAAG GAGTGTTCAA AGAACTCTTT 
ACAGCTTATT CTCGGGAACC TAATAAACCA AAGAAATACG TTCAGGATGT ACTGAGGGAA 
CAGTTATCTG ATGTGACATA CAAAGCTCTG AAGGATCAAG GGGGACACAT ATATATTTGT 
GGTGATGTCA ACATGGCTGG GGATGTCCTG AAGAGTCTTC AGCATATTGT GAAAGAGCGT 
GGAAATCTGA CAGCTGAAGA AGCTGGGGCC TTCATCAGCA AACTAAGAGA TGACAATCGG 
TACCATGAAG ACATTTTTGG AGTCACTCTT AGGACGTATG AAGTTACAAA TCGTCTGAGA 
TCTGAATCTA TTGCATTTAT TGAGGAAAGC AAAAAGGACT CAGATGAGGT TTTCTGCTCA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nos1 ( XM_031892791.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031748651.1 Xenopus tropicalis nitric oxide synthase 1 (nos1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa03509
Clone ID Related Accession (Same CDS sequence)	XM_031892799.1 , XM_031892791.1 , XM_031892798.1 , XM_004910501.3 , XM_031892807.1
Accession Version	XM_031892791.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4263bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nitric oxide synthase, brain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAAT ATGAATTCAG CGTTAAACAA CTGCAGCCGA ATGTTATATC AGTACGCCTC 
TTTAAACGCA AAGTGGGCGG GCTGGGATTC CTGGTTAAGC AAAGGAGGAA CAAACCACCT 
GTAATTATTT CTGATTTAAT TCGGGGAGGG GCAGCAGAGC AGAGTGGGTT GGTGCAAGTA 
GGGGATATTA TCTTAGCAGT GAATGATAGG CCCCTTGTAG ATGCCAGCTA TGAAAGTGCC 
CTGGAGATAC TGCGTAGCAT TTCCACAGAG ACTTTCGTTG TACTGATCCT TAGAGGTCCA 
GAAGGATTCA CAACCCACTT GGAGACCACC TTTTCTGGAG ATGGGACTCC CAAGACAATT 
CGGGTCACCA GACCTCTGTG TTCTAAGTCC AAGTCAACTG ACTTGACAAG TCAGAGCCTT 
TATAGCAAAG ATCACCAAAA TATGGTGGAC AGTGCCACAA GTTCAGCTGG CTCTTCATGG 
CAGGAGGTTC ATCAGCTTCT TAACTTAAAT GGACTTGATG GTGGTAAAGG TGATGACATC 
AGTATCAGCC CTTGCCTTAA TGGAGGAGAA AAAGCTAATG ACATATTGAA AGAAATTGAG 
CCAGTTGTGA GCCTTCTGAA TGCAGGAATG GACCTAAATG GGGATGGACA TCGAAACGTG 
AAGCAAAAGG ATGCGGAAGT TCAGGTTGAA AGCAACTCGC AGACACAACC AAGCACGCAG 
AAGGACCAAG TCAATGGTAT CTGGAAAAAC AACAACAAGA TGCCTGTGGT TTTAAACAAC 
CCATATCTGG AGTGTGAGCA GGTGACATTA ACTGGTAGAC ATTCCCCTGC AAAGAGCCAA 
GTGAATGGTA GTCCCTCCAA ATGTCCTCGT TATATGAAAA TTCGTAACTG GGAGACAAAC 
ACTTCACTTA ATGACACGCT GCACTGCAAG GCTTCCATGC CAACACCCTG CACACAACAG 
ATATGCATGG GGGCAGTGAT GACGCCACCA CATTATGTTC GCAAACCAGA GGACGTCCGG 
ACCAAGGAGG AGCTTCTTCC CCTTGCTAAG GAATTCATTG ACCAATATTA CTCTTCAATG 
AAAAGATTTG GCTCCAAGGC ACACATGGAC AGACTACAAG AAGTAACAAA GGAGATTGAA 
GCTACAGATA CTTATCAGCT ACGAGATACA GAGCTTATTT ATGGAGCCAA GCATGCCTGG 
AGGAATGCAT CCCGATGTGT GGGGAGAATC CAGTGGTCAA AACTGCAGGT ATTTGATGCT 
CGAGACTGCA CCACTGCTCA TGGGATGTTC AATTACATTT GCAATCACAT TAAATATGCC 
ACCAACAAAG GCAACCTCAG ATCAGCAATC ACTATCTTCC CTCAAAGAAC GGATGGGAAG 
CGTGACTTTA GAATCTGGAA TGCTCAGCTT ATTCGCTATG CAGGATACAA ACAGCCTGAT 
GGCTCAGTGC TGGGAGACCC TGCAAATGTA GAATTGACAG AGATCTGCAT TCAGCAGGGC 
TGGAAGGCCC CTTATGGACG TTTTGATGTA CTACCTCTAC TGCTCCAAGC TGATGGCAAT 
GACCCTGAAT TGTTTGAGAT CCCCCCTGAA CTGATCATGG AAGTCAACAT TAGGCACCCC 
AGGCTAGAGT GGTTTAAAGA CCTGGCTTTG AAGTGGTATG GGCTGCCTGC TGTATCCAAT 
TTGCTGCTAG AAGTTGGAGG CTTGGAATTC AGTGCCTGTC CCTTCAGCGG ATGGTACATG 
GGGACAGAAA TTGGTGTTCG AGATTACTGT GACAACTCCC GCTACAACAT ACTGGAGGAA 
GTAGCCCTGA AAATGAATCT GGACACAAGG AAAACATCCT CTTTGTGGAA GGATCAAGCT 
CTGGTGGAGA TAAACATTGC TGTATTGTAC AGTTTCCAGA GTGATAAAAT AACAATCGTC 
GACCATCACT CAGCTACTGA GTCTTTCATC AAACATATGG AGAATGAATA CCGCTGCCGA 
GGAGGCTGTC CTGCAGACTG GGTTTGGATA GTACCGCCGA TGTCTGGAAG CATTACCCCT 
GTATTTCACC AGGAAATGTT AAACTACCGT CTCACACCTT CATTTGAGTA CCAGCCTGAT 
CCATGGAATA CCCACATATG GAAAGGGGTT AATGGAACAC CAACAAAGAA AAGAGCCATT 
GGATTTAAAA AGCTGGCAAA GGCAGTTAAA TTTTCAGCTA AATTAATGGG ACAGGCCATG 
GCTAAAAGAG TAAAGGCTAC AATTCTCTAC GCAACAGAAA CTGGGAAATC CGAAGTCTAT 
GCAAAAACTT TATGTGAAAT CTTCAAGCAT GCATTTGATG CAAAGGTTAT GTCAATGGAT 
GACTATGACA TTGTGCACCT AGAGCATGAA ACACTTGTCT TAGTAGTGAC TAGTACTTTT 
GGTAATGGTG ATCCACCAGA AAATGGGGAG AAGTTTGGCT GTGCATTAAT GGAAATGAGG 
CATCCCAACT CCAACCTGGA GGAGAGGACA AGCTACAAAG TCCGTTTTAA CAGCGTCTCA 
TCCTCTGCTG ATGCCCAGAA ATCGTCTGCT GATGAGACAG GACCCAGAGA CAACTTTGGT 
AGTGCAGGTC CCCTAGCTAA TGTTAGATTC TCAGCGTTTG GACTTGGCTC CCGAGCTTAC 
CCCCACTTCT GTGCCTTTGC CCGTGCGGTT GACACCCTGC TGGAGGAGCT TGGAGGAGAA 
AGAATACTGA AAATGGGGGA AGGGGATGAA TTGTGCGGTC AGGAAGAATC CTTTAGAACA 
TGGGCCAAAA AAGTGTTTCA GGCTGCATGT GACGTCTTTT GTGTAGGAGA TGATGTAAGT 
ATTGAAAAAG CAAACAACTC ACTGATTAGC AATGACCGCA GCTGGAAAAG AAGCAAGTAC 
CGGATCAGCT ATGTGGCAGA GGCACCAGAA CTTACACAAG CATTATACAG TATTCACAAG 
AAGAAAGTGT ACGGAGCTCG ATTGTTGTCT CGCCAGAACT TGCAAAGCCC AAAATCCAGC 
CGATCCACTA TATTCCTTCA ACTTCACACT AACGAACATA AAGAACTGTG CTACAAACCT 
GGAGACCATT TGGGAATCTT TCCTGGAAAC CATGAAGATC TAGTGAATGC CTTGATAGAG 
CAATTAGAAG ATGCACCTCC TGTAAACCAG CTGGTGAGGG TGGAGATGCT GGAAGAAAGA 
AATACTGCTT TAGGTGTAAT TAGTAACTGG ACAGAGGAGC AGCGAATCCC ACCCTGCACC 
ATCTTTCAAG CATTTAAGTA TTTCTTAGAT ATCACCACAC CCCCTACCCC TCTCCTCATG 
CAGCAAATTG CGCTGCTGGC AACTGATGAG AAAGAAAAGA ATCGGCTGGA GATCTTAAGC 
AAGGGCTTGC AGGAATATGA GCAATGGAAG TGGTACAAGA ATCCTACTAT TGTTGAGGTG 
CTAGAAGAGT TTCCTTCAAT TCAGATGCCT TCATCCCTGC TGCTTACACA ACTACCCTTG 
CTACAGCCTC GATACTACTC CATCAGCTCA TCACCTGAGA TGTATCCGGA AGAAGTGCAC 
CTTACTGTTG CTGTTGTGAC CTACCGGACA AGAGATGGAG ATGGGCCTAT ACATCATGGA 
GTCTGCTCCT CATGGCTCAA CAGAATACAG CCAGATGAAG TTGTGCCCTG CTTTGTCAGA 
GGAGCACCAA GTTTCCAGAT GCCAGAAGAC CCACAGGTCC CCTGCATTCT GATAGGACCA 
GGCACTGGCA TTGCTCCATT TAGGAGTTTT TGGCAGCAAC GTCTCTATGA TATGCAGCAC 
AGAGGACTAA AACCTTGTCC AATGATCTTG GTATTTGGGT GTAGGGAATC CAAAATTGAC 
CACATCTACA AAGAGGAAAC CATGCATGCA ATGAATAAAG GAGTGTTCAA AGAACTCTTT 
ACAGCTTATT CTCGGGAACC TAATAAACCA AAGAAATACG TTCAGGATGT ACTGAGGGAA 
CAGTTATCTG ATGTGACATA CAAAGCTCTG AAGGATCAAG GGGGACACAT ATATATTTGT 
GGTGATGTCA ACATGGCTGG GGATGTCCTG AAGAGTCTTC AGCATATTGT GAAAGAGCGT 
GGAAATCTGA CAGCTGAAGA AGCTGGGGCC TTCATCAGCA AACTAAGAGA TGACAATCGG 
TACCATGAAG ACATTTTTGG AGTCACTCTT AGGACGTATG AAGTTACAAA TCGTCTGAGA 
TCTGAATCTA TTGCATTTAT TGAGGAAAGC AAAAAGGACT CAGATGAGGT TTTCTGCTCA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031748651.1
CDS	825..5087
Translation	MEEYEFSVKQLQPNVISVRLFKRKVGGLGFLVKQRRNKPPVIISDLIRGGAAEQSGLVQVGDIILAVNDRPLVDASYESALEILRSISTETFVVLILRGPEGFTTHLETTFSGDGTPKTIRVTRPLCSKSKSTDLTSQSLYSKDHQNMVDSATSSAGSSWQEVHQLLNLNGLDGGKGDDISISPCLNGGEKANDILKEIEPVVSLLNAGMDLNGDGHRNVKQKDAEVQVESNSQTQPSTQKDQVNGIWKNNNKMPVVLNNPYLECEQVTLTGRHSPAKSQVNGSPSKCPRYMKIRNWETNTSLNDTLHCKASMPTPCTQQICMGAVMTPPHYVRKPEDVRTKEELLPLAKEFIDQYYSSMKRFGSKAHMDRLQEVTKEIEATDTYQLRDTELIYGAKHAWRNASRCVGRIQWSKLQVFDARDCTTAHGMFNYICNHIKYATNKGNLRSAITIFPQRTDGKRDFRIWNAQLIRYAGYKQPDGSVLGDPANVELTEICIQQGWKAPYGRFDVLPLLLQADGNDPELFEIPPELIMEVNIRHPRLEWFKDLALKWYGLPAVSNLLLEVGGLEFSACPFSGWYMGTEIGVRDYCDNSRYNILEEVALKMNLDTRKTSSLWKDQALVEINIAVLYSFQSDKITIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMLNYRLTPSFEYQPDPWNTHIWKGVNGTPTKKRAIGFKKLAKAVKFSAKLMGQAMAKRVKATILYATETGKSEVYAKTLCEIFKHAFDAKVMSMDDYDIVHLEHETLVLVVTSTFGNGDPPENGEKFGCALMEMRHPNSNLEERTSYKVRFNSVSSSADAQKSSADETGPRDNFGSAGPLANVRFSAFGLGSRAYPHFCAFARAVDTLLEELGGERILKMGEGDELCGQEESFRTWAKKVFQAACDVFCVGDDVSIEKANNSLISNDRSWKRSKYRISYVAEAPELTQALYSIHKKKVYGARLLSRQNLQSPKSSRSTIFLQLHTNEHKELCYKPGDHLGIFPGNHEDLVNALIEQLEDAPPVNQLVRVEMLEERNTALGVISNWTEEQRIPPCTIFQAFKYFLDITTPPTPLLMQQIALLATDEKEKNRLEILSKGLQEYEQWKWYKNPTIVEVLEEFPSIQMPSSLLLTQLPLLQPRYYSISSSPEMYPEEVHLTVAVVTYRTRDGDGPIHHGVCSSWLNRIQPDEVVPCFVRGAPSFQMPEDPQVPCILIGPGTGIAPFRSFWQQRLYDMQHRGLKPCPMILVFGCRESKIDHIYKEETMHAMNKGVFKELFTAYSREPNKPKKYVQDVLREQLSDVTYKALKDQGGHIYICGDVNMAGDVLKSLQHIVKERGNLTAEEAGAFISKLRDDNRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDSDEVFCS

Target ORF information:

RefSeq Version	XM_031892791.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nitric oxide synthase 1 (nos1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031892791.1

ORF Insert Sequence:

ATGGAGGAAT ATGAATTCAG CGTTAAACAA CTGCAGCCGA ATGTTATATC AGTACGCCTC 
TTTAAACGCA AAGTGGGCGG GCTGGGATTC CTGGTTAAGC AAAGGAGGAA CAAACCACCT 
GTAATTATTT CTGATTTAAT TCGGGGAGGG GCAGCAGAGC AGAGTGGGTT GGTGCAAGTA 
GGGGATATTA TCTTAGCAGT GAATGATAGG CCCCTTGTAG ATGCCAGCTA TGAAAGTGCC 
CTGGAGATAC TGCGTAGCAT TTCCACAGAG ACTTTCGTTG TACTGATCCT TAGAGGTCCA 
GAAGGATTCA CAACCCACTT GGAGACCACC TTTTCTGGAG ATGGGACTCC CAAGACAATT 
CGGGTCACCA GACCTCTGTG TTCTAAGTCC AAGTCAACTG ACTTGACAAG TCAGAGCCTT 
TATAGCAAAG ATCACCAAAA TATGGTGGAC AGTGCCACAA GTTCAGCTGG CTCTTCATGG 
CAGGAGGTTC ATCAGCTTCT TAACTTAAAT GGACTTGATG GTGGTAAAGG TGATGACATC 
AGTATCAGCC CTTGCCTTAA TGGAGGAGAA AAAGCTAATG ACATATTGAA AGAAATTGAG 
CCAGTTGTGA GCCTTCTGAA TGCAGGAATG GACCTAAATG GGGATGGACA TCGAAACGTG 
AAGCAAAAGG ATGCGGAAGT TCAGGTTGAA AGCAACTCGC AGACACAACC AAGCACGCAG 
AAGGACCAAG TCAATGGTAT CTGGAAAAAC AACAACAAGA TGCCTGTGGT TTTAAACAAC 
CCATATCTGG AGTGTGAGCA GGTGACATTA ACTGGTAGAC ATTCCCCTGC AAAGAGCCAA 
GTGAATGGTA GTCCCTCCAA ATGTCCTCGT TATATGAAAA TTCGTAACTG GGAGACAAAC 
ACTTCACTTA ATGACACGCT GCACTGCAAG GCTTCCATGC CAACACCCTG CACACAACAG 
ATATGCATGG GGGCAGTGAT GACGCCACCA CATTATGTTC GCAAACCAGA GGACGTCCGG 
ACCAAGGAGG AGCTTCTTCC CCTTGCTAAG GAATTCATTG ACCAATATTA CTCTTCAATG 
AAAAGATTTG GCTCCAAGGC ACACATGGAC AGACTACAAG AAGTAACAAA GGAGATTGAA 
GCTACAGATA CTTATCAGCT ACGAGATACA GAGCTTATTT ATGGAGCCAA GCATGCCTGG 
AGGAATGCAT CCCGATGTGT GGGGAGAATC CAGTGGTCAA AACTGCAGGT ATTTGATGCT 
CGAGACTGCA CCACTGCTCA TGGGATGTTC AATTACATTT GCAATCACAT TAAATATGCC 
ACCAACAAAG GCAACCTCAG ATCAGCAATC ACTATCTTCC CTCAAAGAAC GGATGGGAAG 
CGTGACTTTA GAATCTGGAA TGCTCAGCTT ATTCGCTATG CAGGATACAA ACAGCCTGAT 
GGCTCAGTGC TGGGAGACCC TGCAAATGTA GAATTGACAG AGATCTGCAT TCAGCAGGGC 
TGGAAGGCCC CTTATGGACG TTTTGATGTA CTACCTCTAC TGCTCCAAGC TGATGGCAAT 
GACCCTGAAT TGTTTGAGAT CCCCCCTGAA CTGATCATGG AAGTCAACAT TAGGCACCCC 
AGGCTAGAGT GGTTTAAAGA CCTGGCTTTG AAGTGGTATG GGCTGCCTGC TGTATCCAAT 
TTGCTGCTAG AAGTTGGAGG CTTGGAATTC AGTGCCTGTC CCTTCAGCGG ATGGTACATG 
GGGACAGAAA TTGGTGTTCG AGATTACTGT GACAACTCCC GCTACAACAT ACTGGAGGAA 
GTAGCCCTGA AAATGAATCT GGACACAAGG AAAACATCCT CTTTGTGGAA GGATCAAGCT 
CTGGTGGAGA TAAACATTGC TGTATTGTAC AGTTTCCAGA GTGATAAAAT AACAATCGTC 
GACCATCACT CAGCTACTGA GTCTTTCATC AAACATATGG AGAATGAATA CCGCTGCCGA 
GGAGGCTGTC CTGCAGACTG GGTTTGGATA GTACCGCCGA TGTCTGGAAG CATTACCCCT 
GTATTTCACC AGGAAATGTT AAACTACCGT CTCACACCTT CATTTGAGTA CCAGCCTGAT 
CCATGGAATA CCCACATATG GAAAGGGGTT AATGGAACAC CAACAAAGAA AAGAGCCATT 
GGATTTAAAA AGCTGGCAAA GGCAGTTAAA TTTTCAGCTA AATTAATGGG ACAGGCCATG 
GCTAAAAGAG TAAAGGCTAC AATTCTCTAC GCAACAGAAA CTGGGAAATC CGAAGTCTAT 
GCAAAAACTT TATGTGAAAT CTTCAAGCAT GCATTTGATG CAAAGGTTAT GTCAATGGAT 
GACTATGACA TTGTGCACCT AGAGCATGAA ACACTTGTCT TAGTAGTGAC TAGTACTTTT 
GGTAATGGTG ATCCACCAGA AAATGGGGAG AAGTTTGGCT GTGCATTAAT GGAAATGAGG 
CATCCCAACT CCAACCTGGA GGAGAGGACA AGCTACAAAG TCCGTTTTAA CAGCGTCTCA 
TCCTCTGCTG ATGCCCAGAA ATCGTCTGCT GATGAGACAG GACCCAGAGA CAACTTTGGT 
AGTGCAGGTC CCCTAGCTAA TGTTAGATTC TCAGCGTTTG GACTTGGCTC CCGAGCTTAC 
CCCCACTTCT GTGCCTTTGC CCGTGCGGTT GACACCCTGC TGGAGGAGCT TGGAGGAGAA 
AGAATACTGA AAATGGGGGA AGGGGATGAA TTGTGCGGTC AGGAAGAATC CTTTAGAACA 
TGGGCCAAAA AAGTGTTTCA GGCTGCATGT GACGTCTTTT GTGTAGGAGA TGATGTAAGT 
ATTGAAAAAG CAAACAACTC ACTGATTAGC AATGACCGCA GCTGGAAAAG AAGCAAGTAC 
CGGATCAGCT ATGTGGCAGA GGCACCAGAA CTTACACAAG CATTATACAG TATTCACAAG 
AAGAAAGTGT ACGGAGCTCG ATTGTTGTCT CGCCAGAACT TGCAAAGCCC AAAATCCAGC 
CGATCCACTA TATTCCTTCA ACTTCACACT AACGAACATA AAGAACTGTG CTACAAACCT 
GGAGACCATT TGGGAATCTT TCCTGGAAAC CATGAAGATC TAGTGAATGC CTTGATAGAG 
CAATTAGAAG ATGCACCTCC TGTAAACCAG CTGGTGAGGG TGGAGATGCT GGAAGAAAGA 
AATACTGCTT TAGGTGTAAT TAGTAACTGG ACAGAGGAGC AGCGAATCCC ACCCTGCACC 
ATCTTTCAAG CATTTAAGTA TTTCTTAGAT ATCACCACAC CCCCTACCCC TCTCCTCATG 
CAGCAAATTG CGCTGCTGGC AACTGATGAG AAAGAAAAGA ATCGGCTGGA GATCTTAAGC 
AAGGGCTTGC AGGAATATGA GCAATGGAAG TGGTACAAGA ATCCTACTAT TGTTGAGGTG 
CTAGAAGAGT TTCCTTCAAT TCAGATGCCT TCATCCCTGC TGCTTACACA ACTACCCTTG 
CTACAGCCTC GATACTACTC CATCAGCTCA TCACCTGAGA TGTATCCGGA AGAAGTGCAC 
CTTACTGTTG CTGTTGTGAC CTACCGGACA AGAGATGGAG ATGGGCCTAT ACATCATGGA 
GTCTGCTCCT CATGGCTCAA CAGAATACAG CCAGATGAAG TTGTGCCCTG CTTTGTCAGA 
GGAGCACCAA GTTTCCAGAT GCCAGAAGAC CCACAGGTCC CCTGCATTCT GATAGGACCA 
GGCACTGGCA TTGCTCCATT TAGGAGTTTT TGGCAGCAAC GTCTCTATGA TATGCAGCAC 
AGAGGACTAA AACCTTGTCC AATGATCTTG GTATTTGGGT GTAGGGAATC CAAAATTGAC 
CACATCTACA AAGAGGAAAC CATGCATGCA ATGAATAAAG GAGTGTTCAA AGAACTCTTT 
ACAGCTTATT CTCGGGAACC TAATAAACCA AAGAAATACG TTCAGGATGT ACTGAGGGAA 
CAGTTATCTG ATGTGACATA CAAAGCTCTG AAGGATCAAG GGGGACACAT ATATATTTGT 
GGTGATGTCA ACATGGCTGG GGATGTCCTG AAGAGTCTTC AGCATATTGT GAAAGAGCGT 
GGAAATCTGA CAGCTGAAGA AGCTGGGGCC TTCATCAGCA AACTAAGAGA TGACAATCGG 
TACCATGAAG ACATTTTTGG AGTCACTCTT AGGACGTATG AAGTTACAAA TCGTCTGAGA 
TCTGAATCTA TTGCATTTAT TGAGGAAAGC AAAAAGGACT CAGATGAGGT TTTCTGCTCA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nos1 ( XM_031892798.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031748658.1 Xenopus tropicalis nitric oxide synthase 1 (nos1), transcript variant X3, mRNA.

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CloneID	OXa03509
Clone ID Related Accession (Same CDS sequence)	XM_031892799.1 , XM_031892791.1 , XM_031892798.1 , XM_004910501.3 , XM_031892807.1
Accession Version	XM_031892798.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4263bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nitric oxide synthase, brain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAAT ATGAATTCAG CGTTAAACAA CTGCAGCCGA ATGTTATATC AGTACGCCTC 
TTTAAACGCA AAGTGGGCGG GCTGGGATTC CTGGTTAAGC AAAGGAGGAA CAAACCACCT 
GTAATTATTT CTGATTTAAT TCGGGGAGGG GCAGCAGAGC AGAGTGGGTT GGTGCAAGTA 
GGGGATATTA TCTTAGCAGT GAATGATAGG CCCCTTGTAG ATGCCAGCTA TGAAAGTGCC 
CTGGAGATAC TGCGTAGCAT TTCCACAGAG ACTTTCGTTG TACTGATCCT TAGAGGTCCA 
GAAGGATTCA CAACCCACTT GGAGACCACC TTTTCTGGAG ATGGGACTCC CAAGACAATT 
CGGGTCACCA GACCTCTGTG TTCTAAGTCC AAGTCAACTG ACTTGACAAG TCAGAGCCTT 
TATAGCAAAG ATCACCAAAA TATGGTGGAC AGTGCCACAA GTTCAGCTGG CTCTTCATGG 
CAGGAGGTTC ATCAGCTTCT TAACTTAAAT GGACTTGATG GTGGTAAAGG TGATGACATC 
AGTATCAGCC CTTGCCTTAA TGGAGGAGAA AAAGCTAATG ACATATTGAA AGAAATTGAG 
CCAGTTGTGA GCCTTCTGAA TGCAGGAATG GACCTAAATG GGGATGGACA TCGAAACGTG 
AAGCAAAAGG ATGCGGAAGT TCAGGTTGAA AGCAACTCGC AGACACAACC AAGCACGCAG 
AAGGACCAAG TCAATGGTAT CTGGAAAAAC AACAACAAGA TGCCTGTGGT TTTAAACAAC 
CCATATCTGG AGTGTGAGCA GGTGACATTA ACTGGTAGAC ATTCCCCTGC AAAGAGCCAA 
GTGAATGGTA GTCCCTCCAA ATGTCCTCGT TATATGAAAA TTCGTAACTG GGAGACAAAC 
ACTTCACTTA ATGACACGCT GCACTGCAAG GCTTCCATGC CAACACCCTG CACACAACAG 
ATATGCATGG GGGCAGTGAT GACGCCACCA CATTATGTTC GCAAACCAGA GGACGTCCGG 
ACCAAGGAGG AGCTTCTTCC CCTTGCTAAG GAATTCATTG ACCAATATTA CTCTTCAATG 
AAAAGATTTG GCTCCAAGGC ACACATGGAC AGACTACAAG AAGTAACAAA GGAGATTGAA 
GCTACAGATA CTTATCAGCT ACGAGATACA GAGCTTATTT ATGGAGCCAA GCATGCCTGG 
AGGAATGCAT CCCGATGTGT GGGGAGAATC CAGTGGTCAA AACTGCAGGT ATTTGATGCT 
CGAGACTGCA CCACTGCTCA TGGGATGTTC AATTACATTT GCAATCACAT TAAATATGCC 
ACCAACAAAG GCAACCTCAG ATCAGCAATC ACTATCTTCC CTCAAAGAAC GGATGGGAAG 
CGTGACTTTA GAATCTGGAA TGCTCAGCTT ATTCGCTATG CAGGATACAA ACAGCCTGAT 
GGCTCAGTGC TGGGAGACCC TGCAAATGTA GAATTGACAG AGATCTGCAT TCAGCAGGGC 
TGGAAGGCCC CTTATGGACG TTTTGATGTA CTACCTCTAC TGCTCCAAGC TGATGGCAAT 
GACCCTGAAT TGTTTGAGAT CCCCCCTGAA CTGATCATGG AAGTCAACAT TAGGCACCCC 
AGGCTAGAGT GGTTTAAAGA CCTGGCTTTG AAGTGGTATG GGCTGCCTGC TGTATCCAAT 
TTGCTGCTAG AAGTTGGAGG CTTGGAATTC AGTGCCTGTC CCTTCAGCGG ATGGTACATG 
GGGACAGAAA TTGGTGTTCG AGATTACTGT GACAACTCCC GCTACAACAT ACTGGAGGAA 
GTAGCCCTGA AAATGAATCT GGACACAAGG AAAACATCCT CTTTGTGGAA GGATCAAGCT 
CTGGTGGAGA TAAACATTGC TGTATTGTAC AGTTTCCAGA GTGATAAAAT AACAATCGTC 
GACCATCACT CAGCTACTGA GTCTTTCATC AAACATATGG AGAATGAATA CCGCTGCCGA 
GGAGGCTGTC CTGCAGACTG GGTTTGGATA GTACCGCCGA TGTCTGGAAG CATTACCCCT 
GTATTTCACC AGGAAATGTT AAACTACCGT CTCACACCTT CATTTGAGTA CCAGCCTGAT 
CCATGGAATA CCCACATATG GAAAGGGGTT AATGGAACAC CAACAAAGAA AAGAGCCATT 
GGATTTAAAA AGCTGGCAAA GGCAGTTAAA TTTTCAGCTA AATTAATGGG ACAGGCCATG 
GCTAAAAGAG TAAAGGCTAC AATTCTCTAC GCAACAGAAA CTGGGAAATC CGAAGTCTAT 
GCAAAAACTT TATGTGAAAT CTTCAAGCAT GCATTTGATG CAAAGGTTAT GTCAATGGAT 
GACTATGACA TTGTGCACCT AGAGCATGAA ACACTTGTCT TAGTAGTGAC TAGTACTTTT 
GGTAATGGTG ATCCACCAGA AAATGGGGAG AAGTTTGGCT GTGCATTAAT GGAAATGAGG 
CATCCCAACT CCAACCTGGA GGAGAGGACA AGCTACAAAG TCCGTTTTAA CAGCGTCTCA 
TCCTCTGCTG ATGCCCAGAA ATCGTCTGCT GATGAGACAG GACCCAGAGA CAACTTTGGT 
AGTGCAGGTC CCCTAGCTAA TGTTAGATTC TCAGCGTTTG GACTTGGCTC CCGAGCTTAC 
CCCCACTTCT GTGCCTTTGC CCGTGCGGTT GACACCCTGC TGGAGGAGCT TGGAGGAGAA 
AGAATACTGA AAATGGGGGA AGGGGATGAA TTGTGCGGTC AGGAAGAATC CTTTAGAACA 
TGGGCCAAAA AAGTGTTTCA GGCTGCATGT GACGTCTTTT GTGTAGGAGA TGATGTAAGT 
ATTGAAAAAG CAAACAACTC ACTGATTAGC AATGACCGCA GCTGGAAAAG AAGCAAGTAC 
CGGATCAGCT ATGTGGCAGA GGCACCAGAA CTTACACAAG CATTATACAG TATTCACAAG 
AAGAAAGTGT ACGGAGCTCG ATTGTTGTCT CGCCAGAACT TGCAAAGCCC AAAATCCAGC 
CGATCCACTA TATTCCTTCA ACTTCACACT AACGAACATA AAGAACTGTG CTACAAACCT 
GGAGACCATT TGGGAATCTT TCCTGGAAAC CATGAAGATC TAGTGAATGC CTTGATAGAG 
CAATTAGAAG ATGCACCTCC TGTAAACCAG CTGGTGAGGG TGGAGATGCT GGAAGAAAGA 
AATACTGCTT TAGGTGTAAT TAGTAACTGG ACAGAGGAGC AGCGAATCCC ACCCTGCACC 
ATCTTTCAAG CATTTAAGTA TTTCTTAGAT ATCACCACAC CCCCTACCCC TCTCCTCATG 
CAGCAAATTG CGCTGCTGGC AACTGATGAG AAAGAAAAGA ATCGGCTGGA GATCTTAAGC 
AAGGGCTTGC AGGAATATGA GCAATGGAAG TGGTACAAGA ATCCTACTAT TGTTGAGGTG 
CTAGAAGAGT TTCCTTCAAT TCAGATGCCT TCATCCCTGC TGCTTACACA ACTACCCTTG 
CTACAGCCTC GATACTACTC CATCAGCTCA TCACCTGAGA TGTATCCGGA AGAAGTGCAC 
CTTACTGTTG CTGTTGTGAC CTACCGGACA AGAGATGGAG ATGGGCCTAT ACATCATGGA 
GTCTGCTCCT CATGGCTCAA CAGAATACAG CCAGATGAAG TTGTGCCCTG CTTTGTCAGA 
GGAGCACCAA GTTTCCAGAT GCCAGAAGAC CCACAGGTCC CCTGCATTCT GATAGGACCA 
GGCACTGGCA TTGCTCCATT TAGGAGTTTT TGGCAGCAAC GTCTCTATGA TATGCAGCAC 
AGAGGACTAA AACCTTGTCC AATGATCTTG GTATTTGGGT GTAGGGAATC CAAAATTGAC 
CACATCTACA AAGAGGAAAC CATGCATGCA ATGAATAAAG GAGTGTTCAA AGAACTCTTT 
ACAGCTTATT CTCGGGAACC TAATAAACCA AAGAAATACG TTCAGGATGT ACTGAGGGAA 
CAGTTATCTG ATGTGACATA CAAAGCTCTG AAGGATCAAG GGGGACACAT ATATATTTGT 
GGTGATGTCA ACATGGCTGG GGATGTCCTG AAGAGTCTTC AGCATATTGT GAAAGAGCGT 
GGAAATCTGA CAGCTGAAGA AGCTGGGGCC TTCATCAGCA AACTAAGAGA TGACAATCGG 
TACCATGAAG ACATTTTTGG AGTCACTCTT AGGACGTATG AAGTTACAAA TCGTCTGAGA 
TCTGAATCTA TTGCATTTAT TGAGGAAAGC AAAAAGGACT CAGATGAGGT TTTCTGCTCA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031748658.1
CDS	589..4851
Translation	MEEYEFSVKQLQPNVISVRLFKRKVGGLGFLVKQRRNKPPVIISDLIRGGAAEQSGLVQVGDIILAVNDRPLVDASYESALEILRSISTETFVVLILRGPEGFTTHLETTFSGDGTPKTIRVTRPLCSKSKSTDLTSQSLYSKDHQNMVDSATSSAGSSWQEVHQLLNLNGLDGGKGDDISISPCLNGGEKANDILKEIEPVVSLLNAGMDLNGDGHRNVKQKDAEVQVESNSQTQPSTQKDQVNGIWKNNNKMPVVLNNPYLECEQVTLTGRHSPAKSQVNGSPSKCPRYMKIRNWETNTSLNDTLHCKASMPTPCTQQICMGAVMTPPHYVRKPEDVRTKEELLPLAKEFIDQYYSSMKRFGSKAHMDRLQEVTKEIEATDTYQLRDTELIYGAKHAWRNASRCVGRIQWSKLQVFDARDCTTAHGMFNYICNHIKYATNKGNLRSAITIFPQRTDGKRDFRIWNAQLIRYAGYKQPDGSVLGDPANVELTEICIQQGWKAPYGRFDVLPLLLQADGNDPELFEIPPELIMEVNIRHPRLEWFKDLALKWYGLPAVSNLLLEVGGLEFSACPFSGWYMGTEIGVRDYCDNSRYNILEEVALKMNLDTRKTSSLWKDQALVEINIAVLYSFQSDKITIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMLNYRLTPSFEYQPDPWNTHIWKGVNGTPTKKRAIGFKKLAKAVKFSAKLMGQAMAKRVKATILYATETGKSEVYAKTLCEIFKHAFDAKVMSMDDYDIVHLEHETLVLVVTSTFGNGDPPENGEKFGCALMEMRHPNSNLEERTSYKVRFNSVSSSADAQKSSADETGPRDNFGSAGPLANVRFSAFGLGSRAYPHFCAFARAVDTLLEELGGERILKMGEGDELCGQEESFRTWAKKVFQAACDVFCVGDDVSIEKANNSLISNDRSWKRSKYRISYVAEAPELTQALYSIHKKKVYGARLLSRQNLQSPKSSRSTIFLQLHTNEHKELCYKPGDHLGIFPGNHEDLVNALIEQLEDAPPVNQLVRVEMLEERNTALGVISNWTEEQRIPPCTIFQAFKYFLDITTPPTPLLMQQIALLATDEKEKNRLEILSKGLQEYEQWKWYKNPTIVEVLEEFPSIQMPSSLLLTQLPLLQPRYYSISSSPEMYPEEVHLTVAVVTYRTRDGDGPIHHGVCSSWLNRIQPDEVVPCFVRGAPSFQMPEDPQVPCILIGPGTGIAPFRSFWQQRLYDMQHRGLKPCPMILVFGCRESKIDHIYKEETMHAMNKGVFKELFTAYSREPNKPKKYVQDVLREQLSDVTYKALKDQGGHIYICGDVNMAGDVLKSLQHIVKERGNLTAEEAGAFISKLRDDNRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDSDEVFCS

Target ORF information:

RefSeq Version	XM_031892798.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nitric oxide synthase 1 (nos1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031892798.1

ORF Insert Sequence:

ATGGAGGAAT ATGAATTCAG CGTTAAACAA CTGCAGCCGA ATGTTATATC AGTACGCCTC 
TTTAAACGCA AAGTGGGCGG GCTGGGATTC CTGGTTAAGC AAAGGAGGAA CAAACCACCT 
GTAATTATTT CTGATTTAAT TCGGGGAGGG GCAGCAGAGC AGAGTGGGTT GGTGCAAGTA 
GGGGATATTA TCTTAGCAGT GAATGATAGG CCCCTTGTAG ATGCCAGCTA TGAAAGTGCC 
CTGGAGATAC TGCGTAGCAT TTCCACAGAG ACTTTCGTTG TACTGATCCT TAGAGGTCCA 
GAAGGATTCA CAACCCACTT GGAGACCACC TTTTCTGGAG ATGGGACTCC CAAGACAATT 
CGGGTCACCA GACCTCTGTG TTCTAAGTCC AAGTCAACTG ACTTGACAAG TCAGAGCCTT 
TATAGCAAAG ATCACCAAAA TATGGTGGAC AGTGCCACAA GTTCAGCTGG CTCTTCATGG 
CAGGAGGTTC ATCAGCTTCT TAACTTAAAT GGACTTGATG GTGGTAAAGG TGATGACATC 
AGTATCAGCC CTTGCCTTAA TGGAGGAGAA AAAGCTAATG ACATATTGAA AGAAATTGAG 
CCAGTTGTGA GCCTTCTGAA TGCAGGAATG GACCTAAATG GGGATGGACA TCGAAACGTG 
AAGCAAAAGG ATGCGGAAGT TCAGGTTGAA AGCAACTCGC AGACACAACC AAGCACGCAG 
AAGGACCAAG TCAATGGTAT CTGGAAAAAC AACAACAAGA TGCCTGTGGT TTTAAACAAC 
CCATATCTGG AGTGTGAGCA GGTGACATTA ACTGGTAGAC ATTCCCCTGC AAAGAGCCAA 
GTGAATGGTA GTCCCTCCAA ATGTCCTCGT TATATGAAAA TTCGTAACTG GGAGACAAAC 
ACTTCACTTA ATGACACGCT GCACTGCAAG GCTTCCATGC CAACACCCTG CACACAACAG 
ATATGCATGG GGGCAGTGAT GACGCCACCA CATTATGTTC GCAAACCAGA GGACGTCCGG 
ACCAAGGAGG AGCTTCTTCC CCTTGCTAAG GAATTCATTG ACCAATATTA CTCTTCAATG 
AAAAGATTTG GCTCCAAGGC ACACATGGAC AGACTACAAG AAGTAACAAA GGAGATTGAA 
GCTACAGATA CTTATCAGCT ACGAGATACA GAGCTTATTT ATGGAGCCAA GCATGCCTGG 
AGGAATGCAT CCCGATGTGT GGGGAGAATC CAGTGGTCAA AACTGCAGGT ATTTGATGCT 
CGAGACTGCA CCACTGCTCA TGGGATGTTC AATTACATTT GCAATCACAT TAAATATGCC 
ACCAACAAAG GCAACCTCAG ATCAGCAATC ACTATCTTCC CTCAAAGAAC GGATGGGAAG 
CGTGACTTTA GAATCTGGAA TGCTCAGCTT ATTCGCTATG CAGGATACAA ACAGCCTGAT 
GGCTCAGTGC TGGGAGACCC TGCAAATGTA GAATTGACAG AGATCTGCAT TCAGCAGGGC 
TGGAAGGCCC CTTATGGACG TTTTGATGTA CTACCTCTAC TGCTCCAAGC TGATGGCAAT 
GACCCTGAAT TGTTTGAGAT CCCCCCTGAA CTGATCATGG AAGTCAACAT TAGGCACCCC 
AGGCTAGAGT GGTTTAAAGA CCTGGCTTTG AAGTGGTATG GGCTGCCTGC TGTATCCAAT 
TTGCTGCTAG AAGTTGGAGG CTTGGAATTC AGTGCCTGTC CCTTCAGCGG ATGGTACATG 
GGGACAGAAA TTGGTGTTCG AGATTACTGT GACAACTCCC GCTACAACAT ACTGGAGGAA 
GTAGCCCTGA AAATGAATCT GGACACAAGG AAAACATCCT CTTTGTGGAA GGATCAAGCT 
CTGGTGGAGA TAAACATTGC TGTATTGTAC AGTTTCCAGA GTGATAAAAT AACAATCGTC 
GACCATCACT CAGCTACTGA GTCTTTCATC AAACATATGG AGAATGAATA CCGCTGCCGA 
GGAGGCTGTC CTGCAGACTG GGTTTGGATA GTACCGCCGA TGTCTGGAAG CATTACCCCT 
GTATTTCACC AGGAAATGTT AAACTACCGT CTCACACCTT CATTTGAGTA CCAGCCTGAT 
CCATGGAATA CCCACATATG GAAAGGGGTT AATGGAACAC CAACAAAGAA AAGAGCCATT 
GGATTTAAAA AGCTGGCAAA GGCAGTTAAA TTTTCAGCTA AATTAATGGG ACAGGCCATG 
GCTAAAAGAG TAAAGGCTAC AATTCTCTAC GCAACAGAAA CTGGGAAATC CGAAGTCTAT 
GCAAAAACTT TATGTGAAAT CTTCAAGCAT GCATTTGATG CAAAGGTTAT GTCAATGGAT 
GACTATGACA TTGTGCACCT AGAGCATGAA ACACTTGTCT TAGTAGTGAC TAGTACTTTT 
GGTAATGGTG ATCCACCAGA AAATGGGGAG AAGTTTGGCT GTGCATTAAT GGAAATGAGG 
CATCCCAACT CCAACCTGGA GGAGAGGACA AGCTACAAAG TCCGTTTTAA CAGCGTCTCA 
TCCTCTGCTG ATGCCCAGAA ATCGTCTGCT GATGAGACAG GACCCAGAGA CAACTTTGGT 
AGTGCAGGTC CCCTAGCTAA TGTTAGATTC TCAGCGTTTG GACTTGGCTC CCGAGCTTAC 
CCCCACTTCT GTGCCTTTGC CCGTGCGGTT GACACCCTGC TGGAGGAGCT TGGAGGAGAA 
AGAATACTGA AAATGGGGGA AGGGGATGAA TTGTGCGGTC AGGAAGAATC CTTTAGAACA 
TGGGCCAAAA AAGTGTTTCA GGCTGCATGT GACGTCTTTT GTGTAGGAGA TGATGTAAGT 
ATTGAAAAAG CAAACAACTC ACTGATTAGC AATGACCGCA GCTGGAAAAG AAGCAAGTAC 
CGGATCAGCT ATGTGGCAGA GGCACCAGAA CTTACACAAG CATTATACAG TATTCACAAG 
AAGAAAGTGT ACGGAGCTCG ATTGTTGTCT CGCCAGAACT TGCAAAGCCC AAAATCCAGC 
CGATCCACTA TATTCCTTCA ACTTCACACT AACGAACATA AAGAACTGTG CTACAAACCT 
GGAGACCATT TGGGAATCTT TCCTGGAAAC CATGAAGATC TAGTGAATGC CTTGATAGAG 
CAATTAGAAG ATGCACCTCC TGTAAACCAG CTGGTGAGGG TGGAGATGCT GGAAGAAAGA 
AATACTGCTT TAGGTGTAAT TAGTAACTGG ACAGAGGAGC AGCGAATCCC ACCCTGCACC 
ATCTTTCAAG CATTTAAGTA TTTCTTAGAT ATCACCACAC CCCCTACCCC TCTCCTCATG 
CAGCAAATTG CGCTGCTGGC AACTGATGAG AAAGAAAAGA ATCGGCTGGA GATCTTAAGC 
AAGGGCTTGC AGGAATATGA GCAATGGAAG TGGTACAAGA ATCCTACTAT TGTTGAGGTG 
CTAGAAGAGT TTCCTTCAAT TCAGATGCCT TCATCCCTGC TGCTTACACA ACTACCCTTG 
CTACAGCCTC GATACTACTC CATCAGCTCA TCACCTGAGA TGTATCCGGA AGAAGTGCAC 
CTTACTGTTG CTGTTGTGAC CTACCGGACA AGAGATGGAG ATGGGCCTAT ACATCATGGA 
GTCTGCTCCT CATGGCTCAA CAGAATACAG CCAGATGAAG TTGTGCCCTG CTTTGTCAGA 
GGAGCACCAA GTTTCCAGAT GCCAGAAGAC CCACAGGTCC CCTGCATTCT GATAGGACCA 
GGCACTGGCA TTGCTCCATT TAGGAGTTTT TGGCAGCAAC GTCTCTATGA TATGCAGCAC 
AGAGGACTAA AACCTTGTCC AATGATCTTG GTATTTGGGT GTAGGGAATC CAAAATTGAC 
CACATCTACA AAGAGGAAAC CATGCATGCA ATGAATAAAG GAGTGTTCAA AGAACTCTTT 
ACAGCTTATT CTCGGGAACC TAATAAACCA AAGAAATACG TTCAGGATGT ACTGAGGGAA 
CAGTTATCTG ATGTGACATA CAAAGCTCTG AAGGATCAAG GGGGACACAT ATATATTTGT 
GGTGATGTCA ACATGGCTGG GGATGTCCTG AAGAGTCTTC AGCATATTGT GAAAGAGCGT 
GGAAATCTGA CAGCTGAAGA AGCTGGGGCC TTCATCAGCA AACTAAGAGA TGACAATCGG 
TACCATGAAG ACATTTTTGG AGTCACTCTT AGGACGTATG AAGTTACAAA TCGTCTGAGA 
TCTGAATCTA TTGCATTTAT TGAGGAAAGC AAAAAGGACT CAGATGAGGT TTTCTGCTCA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nos1 ( XM_031892807.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031748667.1 Xenopus tropicalis nitric oxide synthase 1 (nos1), transcript variant X5, mRNA.

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CloneID	OXa03509
Clone ID Related Accession (Same CDS sequence)	XM_031892799.1 , XM_031892791.1 , XM_031892798.1 , XM_004910501.3 , XM_031892807.1
Accession Version	XM_031892807.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4263bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nitric oxide synthase, brain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAAT ATGAATTCAG CGTTAAACAA CTGCAGCCGA ATGTTATATC AGTACGCCTC 
TTTAAACGCA AAGTGGGCGG GCTGGGATTC CTGGTTAAGC AAAGGAGGAA CAAACCACCT 
GTAATTATTT CTGATTTAAT TCGGGGAGGG GCAGCAGAGC AGAGTGGGTT GGTGCAAGTA 
GGGGATATTA TCTTAGCAGT GAATGATAGG CCCCTTGTAG ATGCCAGCTA TGAAAGTGCC 
CTGGAGATAC TGCGTAGCAT TTCCACAGAG ACTTTCGTTG TACTGATCCT TAGAGGTCCA 
GAAGGATTCA CAACCCACTT GGAGACCACC TTTTCTGGAG ATGGGACTCC CAAGACAATT 
CGGGTCACCA GACCTCTGTG TTCTAAGTCC AAGTCAACTG ACTTGACAAG TCAGAGCCTT 
TATAGCAAAG ATCACCAAAA TATGGTGGAC AGTGCCACAA GTTCAGCTGG CTCTTCATGG 
CAGGAGGTTC ATCAGCTTCT TAACTTAAAT GGACTTGATG GTGGTAAAGG TGATGACATC 
AGTATCAGCC CTTGCCTTAA TGGAGGAGAA AAAGCTAATG ACATATTGAA AGAAATTGAG 
CCAGTTGTGA GCCTTCTGAA TGCAGGAATG GACCTAAATG GGGATGGACA TCGAAACGTG 
AAGCAAAAGG ATGCGGAAGT TCAGGTTGAA AGCAACTCGC AGACACAACC AAGCACGCAG 
AAGGACCAAG TCAATGGTAT CTGGAAAAAC AACAACAAGA TGCCTGTGGT TTTAAACAAC 
CCATATCTGG AGTGTGAGCA GGTGACATTA ACTGGTAGAC ATTCCCCTGC AAAGAGCCAA 
GTGAATGGTA GTCCCTCCAA ATGTCCTCGT TATATGAAAA TTCGTAACTG GGAGACAAAC 
ACTTCACTTA ATGACACGCT GCACTGCAAG GCTTCCATGC CAACACCCTG CACACAACAG 
ATATGCATGG GGGCAGTGAT GACGCCACCA CATTATGTTC GCAAACCAGA GGACGTCCGG 
ACCAAGGAGG AGCTTCTTCC CCTTGCTAAG GAATTCATTG ACCAATATTA CTCTTCAATG 
AAAAGATTTG GCTCCAAGGC ACACATGGAC AGACTACAAG AAGTAACAAA GGAGATTGAA 
GCTACAGATA CTTATCAGCT ACGAGATACA GAGCTTATTT ATGGAGCCAA GCATGCCTGG 
AGGAATGCAT CCCGATGTGT GGGGAGAATC CAGTGGTCAA AACTGCAGGT ATTTGATGCT 
CGAGACTGCA CCACTGCTCA TGGGATGTTC AATTACATTT GCAATCACAT TAAATATGCC 
ACCAACAAAG GCAACCTCAG ATCAGCAATC ACTATCTTCC CTCAAAGAAC GGATGGGAAG 
CGTGACTTTA GAATCTGGAA TGCTCAGCTT ATTCGCTATG CAGGATACAA ACAGCCTGAT 
GGCTCAGTGC TGGGAGACCC TGCAAATGTA GAATTGACAG AGATCTGCAT TCAGCAGGGC 
TGGAAGGCCC CTTATGGACG TTTTGATGTA CTACCTCTAC TGCTCCAAGC TGATGGCAAT 
GACCCTGAAT TGTTTGAGAT CCCCCCTGAA CTGATCATGG AAGTCAACAT TAGGCACCCC 
AGGCTAGAGT GGTTTAAAGA CCTGGCTTTG AAGTGGTATG GGCTGCCTGC TGTATCCAAT 
TTGCTGCTAG AAGTTGGAGG CTTGGAATTC AGTGCCTGTC CCTTCAGCGG ATGGTACATG 
GGGACAGAAA TTGGTGTTCG AGATTACTGT GACAACTCCC GCTACAACAT ACTGGAGGAA 
GTAGCCCTGA AAATGAATCT GGACACAAGG AAAACATCCT CTTTGTGGAA GGATCAAGCT 
CTGGTGGAGA TAAACATTGC TGTATTGTAC AGTTTCCAGA GTGATAAAAT AACAATCGTC 
GACCATCACT CAGCTACTGA GTCTTTCATC AAACATATGG AGAATGAATA CCGCTGCCGA 
GGAGGCTGTC CTGCAGACTG GGTTTGGATA GTACCGCCGA TGTCTGGAAG CATTACCCCT 
GTATTTCACC AGGAAATGTT AAACTACCGT CTCACACCTT CATTTGAGTA CCAGCCTGAT 
CCATGGAATA CCCACATATG GAAAGGGGTT AATGGAACAC CAACAAAGAA AAGAGCCATT 
GGATTTAAAA AGCTGGCAAA GGCAGTTAAA TTTTCAGCTA AATTAATGGG ACAGGCCATG 
GCTAAAAGAG TAAAGGCTAC AATTCTCTAC GCAACAGAAA CTGGGAAATC CGAAGTCTAT 
GCAAAAACTT TATGTGAAAT CTTCAAGCAT GCATTTGATG CAAAGGTTAT GTCAATGGAT 
GACTATGACA TTGTGCACCT AGAGCATGAA ACACTTGTCT TAGTAGTGAC TAGTACTTTT 
GGTAATGGTG ATCCACCAGA AAATGGGGAG AAGTTTGGCT GTGCATTAAT GGAAATGAGG 
CATCCCAACT CCAACCTGGA GGAGAGGACA AGCTACAAAG TCCGTTTTAA CAGCGTCTCA 
TCCTCTGCTG ATGCCCAGAA ATCGTCTGCT GATGAGACAG GACCCAGAGA CAACTTTGGT 
AGTGCAGGTC CCCTAGCTAA TGTTAGATTC TCAGCGTTTG GACTTGGCTC CCGAGCTTAC 
CCCCACTTCT GTGCCTTTGC CCGTGCGGTT GACACCCTGC TGGAGGAGCT TGGAGGAGAA 
AGAATACTGA AAATGGGGGA AGGGGATGAA TTGTGCGGTC AGGAAGAATC CTTTAGAACA 
TGGGCCAAAA AAGTGTTTCA GGCTGCATGT GACGTCTTTT GTGTAGGAGA TGATGTAAGT 
ATTGAAAAAG CAAACAACTC ACTGATTAGC AATGACCGCA GCTGGAAAAG AAGCAAGTAC 
CGGATCAGCT ATGTGGCAGA GGCACCAGAA CTTACACAAG CATTATACAG TATTCACAAG 
AAGAAAGTGT ACGGAGCTCG ATTGTTGTCT CGCCAGAACT TGCAAAGCCC AAAATCCAGC 
CGATCCACTA TATTCCTTCA ACTTCACACT AACGAACATA AAGAACTGTG CTACAAACCT 
GGAGACCATT TGGGAATCTT TCCTGGAAAC CATGAAGATC TAGTGAATGC CTTGATAGAG 
CAATTAGAAG ATGCACCTCC TGTAAACCAG CTGGTGAGGG TGGAGATGCT GGAAGAAAGA 
AATACTGCTT TAGGTGTAAT TAGTAACTGG ACAGAGGAGC AGCGAATCCC ACCCTGCACC 
ATCTTTCAAG CATTTAAGTA TTTCTTAGAT ATCACCACAC CCCCTACCCC TCTCCTCATG 
CAGCAAATTG CGCTGCTGGC AACTGATGAG AAAGAAAAGA ATCGGCTGGA GATCTTAAGC 
AAGGGCTTGC AGGAATATGA GCAATGGAAG TGGTACAAGA ATCCTACTAT TGTTGAGGTG 
CTAGAAGAGT TTCCTTCAAT TCAGATGCCT TCATCCCTGC TGCTTACACA ACTACCCTTG 
CTACAGCCTC GATACTACTC CATCAGCTCA TCACCTGAGA TGTATCCGGA AGAAGTGCAC 
CTTACTGTTG CTGTTGTGAC CTACCGGACA AGAGATGGAG ATGGGCCTAT ACATCATGGA 
GTCTGCTCCT CATGGCTCAA CAGAATACAG CCAGATGAAG TTGTGCCCTG CTTTGTCAGA 
GGAGCACCAA GTTTCCAGAT GCCAGAAGAC CCACAGGTCC CCTGCATTCT GATAGGACCA 
GGCACTGGCA TTGCTCCATT TAGGAGTTTT TGGCAGCAAC GTCTCTATGA TATGCAGCAC 
AGAGGACTAA AACCTTGTCC AATGATCTTG GTATTTGGGT GTAGGGAATC CAAAATTGAC 
CACATCTACA AAGAGGAAAC CATGCATGCA ATGAATAAAG GAGTGTTCAA AGAACTCTTT 
ACAGCTTATT CTCGGGAACC TAATAAACCA AAGAAATACG TTCAGGATGT ACTGAGGGAA 
CAGTTATCTG ATGTGACATA CAAAGCTCTG AAGGATCAAG GGGGACACAT ATATATTTGT 
GGTGATGTCA ACATGGCTGG GGATGTCCTG AAGAGTCTTC AGCATATTGT GAAAGAGCGT 
GGAAATCTGA CAGCTGAAGA AGCTGGGGCC TTCATCAGCA AACTAAGAGA TGACAATCGG 
TACCATGAAG ACATTTTTGG AGTCACTCTT AGGACGTATG AAGTTACAAA TCGTCTGAGA 
TCTGAATCTA TTGCATTTAT TGAGGAAAGC AAAAAGGACT CAGATGAGGT TTTCTGCTCA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031748667.1
CDS	681..4943
Translation	MEEYEFSVKQLQPNVISVRLFKRKVGGLGFLVKQRRNKPPVIISDLIRGGAAEQSGLVQVGDIILAVNDRPLVDASYESALEILRSISTETFVVLILRGPEGFTTHLETTFSGDGTPKTIRVTRPLCSKSKSTDLTSQSLYSKDHQNMVDSATSSAGSSWQEVHQLLNLNGLDGGKGDDISISPCLNGGEKANDILKEIEPVVSLLNAGMDLNGDGHRNVKQKDAEVQVESNSQTQPSTQKDQVNGIWKNNNKMPVVLNNPYLECEQVTLTGRHSPAKSQVNGSPSKCPRYMKIRNWETNTSLNDTLHCKASMPTPCTQQICMGAVMTPPHYVRKPEDVRTKEELLPLAKEFIDQYYSSMKRFGSKAHMDRLQEVTKEIEATDTYQLRDTELIYGAKHAWRNASRCVGRIQWSKLQVFDARDCTTAHGMFNYICNHIKYATNKGNLRSAITIFPQRTDGKRDFRIWNAQLIRYAGYKQPDGSVLGDPANVELTEICIQQGWKAPYGRFDVLPLLLQADGNDPELFEIPPELIMEVNIRHPRLEWFKDLALKWYGLPAVSNLLLEVGGLEFSACPFSGWYMGTEIGVRDYCDNSRYNILEEVALKMNLDTRKTSSLWKDQALVEINIAVLYSFQSDKITIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMLNYRLTPSFEYQPDPWNTHIWKGVNGTPTKKRAIGFKKLAKAVKFSAKLMGQAMAKRVKATILYATETGKSEVYAKTLCEIFKHAFDAKVMSMDDYDIVHLEHETLVLVVTSTFGNGDPPENGEKFGCALMEMRHPNSNLEERTSYKVRFNSVSSSADAQKSSADETGPRDNFGSAGPLANVRFSAFGLGSRAYPHFCAFARAVDTLLEELGGERILKMGEGDELCGQEESFRTWAKKVFQAACDVFCVGDDVSIEKANNSLISNDRSWKRSKYRISYVAEAPELTQALYSIHKKKVYGARLLSRQNLQSPKSSRSTIFLQLHTNEHKELCYKPGDHLGIFPGNHEDLVNALIEQLEDAPPVNQLVRVEMLEERNTALGVISNWTEEQRIPPCTIFQAFKYFLDITTPPTPLLMQQIALLATDEKEKNRLEILSKGLQEYEQWKWYKNPTIVEVLEEFPSIQMPSSLLLTQLPLLQPRYYSISSSPEMYPEEVHLTVAVVTYRTRDGDGPIHHGVCSSWLNRIQPDEVVPCFVRGAPSFQMPEDPQVPCILIGPGTGIAPFRSFWQQRLYDMQHRGLKPCPMILVFGCRESKIDHIYKEETMHAMNKGVFKELFTAYSREPNKPKKYVQDVLREQLSDVTYKALKDQGGHIYICGDVNMAGDVLKSLQHIVKERGNLTAEEAGAFISKLRDDNRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDSDEVFCS

Target ORF information:

RefSeq Version	XM_031892807.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nitric oxide synthase 1 (nos1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031892807.1

ORF Insert Sequence:

ATGGAGGAAT ATGAATTCAG CGTTAAACAA CTGCAGCCGA ATGTTATATC AGTACGCCTC 
TTTAAACGCA AAGTGGGCGG GCTGGGATTC CTGGTTAAGC AAAGGAGGAA CAAACCACCT 
GTAATTATTT CTGATTTAAT TCGGGGAGGG GCAGCAGAGC AGAGTGGGTT GGTGCAAGTA 
GGGGATATTA TCTTAGCAGT GAATGATAGG CCCCTTGTAG ATGCCAGCTA TGAAAGTGCC 
CTGGAGATAC TGCGTAGCAT TTCCACAGAG ACTTTCGTTG TACTGATCCT TAGAGGTCCA 
GAAGGATTCA CAACCCACTT GGAGACCACC TTTTCTGGAG ATGGGACTCC CAAGACAATT 
CGGGTCACCA GACCTCTGTG TTCTAAGTCC AAGTCAACTG ACTTGACAAG TCAGAGCCTT 
TATAGCAAAG ATCACCAAAA TATGGTGGAC AGTGCCACAA GTTCAGCTGG CTCTTCATGG 
CAGGAGGTTC ATCAGCTTCT TAACTTAAAT GGACTTGATG GTGGTAAAGG TGATGACATC 
AGTATCAGCC CTTGCCTTAA TGGAGGAGAA AAAGCTAATG ACATATTGAA AGAAATTGAG 
CCAGTTGTGA GCCTTCTGAA TGCAGGAATG GACCTAAATG GGGATGGACA TCGAAACGTG 
AAGCAAAAGG ATGCGGAAGT TCAGGTTGAA AGCAACTCGC AGACACAACC AAGCACGCAG 
AAGGACCAAG TCAATGGTAT CTGGAAAAAC AACAACAAGA TGCCTGTGGT TTTAAACAAC 
CCATATCTGG AGTGTGAGCA GGTGACATTA ACTGGTAGAC ATTCCCCTGC AAAGAGCCAA 
GTGAATGGTA GTCCCTCCAA ATGTCCTCGT TATATGAAAA TTCGTAACTG GGAGACAAAC 
ACTTCACTTA ATGACACGCT GCACTGCAAG GCTTCCATGC CAACACCCTG CACACAACAG 
ATATGCATGG GGGCAGTGAT GACGCCACCA CATTATGTTC GCAAACCAGA GGACGTCCGG 
ACCAAGGAGG AGCTTCTTCC CCTTGCTAAG GAATTCATTG ACCAATATTA CTCTTCAATG 
AAAAGATTTG GCTCCAAGGC ACACATGGAC AGACTACAAG AAGTAACAAA GGAGATTGAA 
GCTACAGATA CTTATCAGCT ACGAGATACA GAGCTTATTT ATGGAGCCAA GCATGCCTGG 
AGGAATGCAT CCCGATGTGT GGGGAGAATC CAGTGGTCAA AACTGCAGGT ATTTGATGCT 
CGAGACTGCA CCACTGCTCA TGGGATGTTC AATTACATTT GCAATCACAT TAAATATGCC 
ACCAACAAAG GCAACCTCAG ATCAGCAATC ACTATCTTCC CTCAAAGAAC GGATGGGAAG 
CGTGACTTTA GAATCTGGAA TGCTCAGCTT ATTCGCTATG CAGGATACAA ACAGCCTGAT 
GGCTCAGTGC TGGGAGACCC TGCAAATGTA GAATTGACAG AGATCTGCAT TCAGCAGGGC 
TGGAAGGCCC CTTATGGACG TTTTGATGTA CTACCTCTAC TGCTCCAAGC TGATGGCAAT 
GACCCTGAAT TGTTTGAGAT CCCCCCTGAA CTGATCATGG AAGTCAACAT TAGGCACCCC 
AGGCTAGAGT GGTTTAAAGA CCTGGCTTTG AAGTGGTATG GGCTGCCTGC TGTATCCAAT 
TTGCTGCTAG AAGTTGGAGG CTTGGAATTC AGTGCCTGTC CCTTCAGCGG ATGGTACATG 
GGGACAGAAA TTGGTGTTCG AGATTACTGT GACAACTCCC GCTACAACAT ACTGGAGGAA 
GTAGCCCTGA AAATGAATCT GGACACAAGG AAAACATCCT CTTTGTGGAA GGATCAAGCT 
CTGGTGGAGA TAAACATTGC TGTATTGTAC AGTTTCCAGA GTGATAAAAT AACAATCGTC 
GACCATCACT CAGCTACTGA GTCTTTCATC AAACATATGG AGAATGAATA CCGCTGCCGA 
GGAGGCTGTC CTGCAGACTG GGTTTGGATA GTACCGCCGA TGTCTGGAAG CATTACCCCT 
GTATTTCACC AGGAAATGTT AAACTACCGT CTCACACCTT CATTTGAGTA CCAGCCTGAT 
CCATGGAATA CCCACATATG GAAAGGGGTT AATGGAACAC CAACAAAGAA AAGAGCCATT 
GGATTTAAAA AGCTGGCAAA GGCAGTTAAA TTTTCAGCTA AATTAATGGG ACAGGCCATG 
GCTAAAAGAG TAAAGGCTAC AATTCTCTAC GCAACAGAAA CTGGGAAATC CGAAGTCTAT 
GCAAAAACTT TATGTGAAAT CTTCAAGCAT GCATTTGATG CAAAGGTTAT GTCAATGGAT 
GACTATGACA TTGTGCACCT AGAGCATGAA ACACTTGTCT TAGTAGTGAC TAGTACTTTT 
GGTAATGGTG ATCCACCAGA AAATGGGGAG AAGTTTGGCT GTGCATTAAT GGAAATGAGG 
CATCCCAACT CCAACCTGGA GGAGAGGACA AGCTACAAAG TCCGTTTTAA CAGCGTCTCA 
TCCTCTGCTG ATGCCCAGAA ATCGTCTGCT GATGAGACAG GACCCAGAGA CAACTTTGGT 
AGTGCAGGTC CCCTAGCTAA TGTTAGATTC TCAGCGTTTG GACTTGGCTC CCGAGCTTAC 
CCCCACTTCT GTGCCTTTGC CCGTGCGGTT GACACCCTGC TGGAGGAGCT TGGAGGAGAA 
AGAATACTGA AAATGGGGGA AGGGGATGAA TTGTGCGGTC AGGAAGAATC CTTTAGAACA 
TGGGCCAAAA AAGTGTTTCA GGCTGCATGT GACGTCTTTT GTGTAGGAGA TGATGTAAGT 
ATTGAAAAAG CAAACAACTC ACTGATTAGC AATGACCGCA GCTGGAAAAG AAGCAAGTAC 
CGGATCAGCT ATGTGGCAGA GGCACCAGAA CTTACACAAG CATTATACAG TATTCACAAG 
AAGAAAGTGT ACGGAGCTCG ATTGTTGTCT CGCCAGAACT TGCAAAGCCC AAAATCCAGC 
CGATCCACTA TATTCCTTCA ACTTCACACT AACGAACATA AAGAACTGTG CTACAAACCT 
GGAGACCATT TGGGAATCTT TCCTGGAAAC CATGAAGATC TAGTGAATGC CTTGATAGAG 
CAATTAGAAG ATGCACCTCC TGTAAACCAG CTGGTGAGGG TGGAGATGCT GGAAGAAAGA 
AATACTGCTT TAGGTGTAAT TAGTAACTGG ACAGAGGAGC AGCGAATCCC ACCCTGCACC 
ATCTTTCAAG CATTTAAGTA TTTCTTAGAT ATCACCACAC CCCCTACCCC TCTCCTCATG 
CAGCAAATTG CGCTGCTGGC AACTGATGAG AAAGAAAAGA ATCGGCTGGA GATCTTAAGC 
AAGGGCTTGC AGGAATATGA GCAATGGAAG TGGTACAAGA ATCCTACTAT TGTTGAGGTG 
CTAGAAGAGT TTCCTTCAAT TCAGATGCCT TCATCCCTGC TGCTTACACA ACTACCCTTG 
CTACAGCCTC GATACTACTC CATCAGCTCA TCACCTGAGA TGTATCCGGA AGAAGTGCAC 
CTTACTGTTG CTGTTGTGAC CTACCGGACA AGAGATGGAG ATGGGCCTAT ACATCATGGA 
GTCTGCTCCT CATGGCTCAA CAGAATACAG CCAGATGAAG TTGTGCCCTG CTTTGTCAGA 
GGAGCACCAA GTTTCCAGAT GCCAGAAGAC CCACAGGTCC CCTGCATTCT GATAGGACCA 
GGCACTGGCA TTGCTCCATT TAGGAGTTTT TGGCAGCAAC GTCTCTATGA TATGCAGCAC 
AGAGGACTAA AACCTTGTCC AATGATCTTG GTATTTGGGT GTAGGGAATC CAAAATTGAC 
CACATCTACA AAGAGGAAAC CATGCATGCA ATGAATAAAG GAGTGTTCAA AGAACTCTTT 
ACAGCTTATT CTCGGGAACC TAATAAACCA AAGAAATACG TTCAGGATGT ACTGAGGGAA 
CAGTTATCTG ATGTGACATA CAAAGCTCTG AAGGATCAAG GGGGACACAT ATATATTTGT 
GGTGATGTCA ACATGGCTGG GGATGTCCTG AAGAGTCTTC AGCATATTGT GAAAGAGCGT 
GGAAATCTGA CAGCTGAAGA AGCTGGGGCC TTCATCAGCA AACTAAGAGA TGACAATCGG 
TACCATGAAG ACATTTTTGG AGTCACTCTT AGGACGTATG AAGTTACAAA TCGTCTGAGA 
TCTGAATCTA TTGCATTTAT TGAGGAAAGC AAAAAGGACT CAGATGAGGT TTTCTGCTCA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nos1 ( XM_004910501.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004910558.1 Xenopus tropicalis nitric oxide synthase 1 (nos1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa03509
Clone ID Related Accession (Same CDS sequence)	XM_031892799.1 , XM_031892791.1 , XM_031892798.1 , XM_004910501.3 , XM_031892807.1
Accession Version	XM_004910501.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4263bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nitric oxide synthase, brain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004910501.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAAT ATGAATTCAG CGTTAAACAA CTGCAGCCGA ATGTTATATC AGTACGCCTC 
TTTAAACGCA AAGTGGGCGG GCTGGGATTC CTGGTTAAGC AAAGGAGGAA CAAACCACCT 
GTAATTATTT CTGATTTAAT TCGGGGAGGG GCAGCAGAGC AGAGTGGGTT GGTGCAAGTA 
GGGGATATTA TCTTAGCAGT GAATGATAGG CCCCTTGTAG ATGCCAGCTA TGAAAGTGCC 
CTGGAGATAC TGCGTAGCAT TTCCACAGAG ACTTTCGTTG TACTGATCCT TAGAGGTCCA 
GAAGGATTCA CAACCCACTT GGAGACCACC TTTTCTGGAG ATGGGACTCC CAAGACAATT 
CGGGTCACCA GACCTCTGTG TTCTAAGTCC AAGTCAACTG ACTTGACAAG TCAGAGCCTT 
TATAGCAAAG ATCACCAAAA TATGGTGGAC AGTGCCACAA GTTCAGCTGG CTCTTCATGG 
CAGGAGGTTC ATCAGCTTCT TAACTTAAAT GGACTTGATG GTGGTAAAGG TGATGACATC 
AGTATCAGCC CTTGCCTTAA TGGAGGAGAA AAAGCTAATG ACATATTGAA AGAAATTGAG 
CCAGTTGTGA GCCTTCTGAA TGCAGGAATG GACCTAAATG GGGATGGACA TCGAAACGTG 
AAGCAAAAGG ATGCGGAAGT TCAGGTTGAA AGCAACTCGC AGACACAACC AAGCACGCAG 
AAGGACCAAG TCAATGGTAT CTGGAAAAAC AACAACAAGA TGCCTGTGGT TTTAAACAAC 
CCATATCTGG AGTGTGAGCA GGTGACATTA ACTGGTAGAC ATTCCCCTGC AAAGAGCCAA 
GTGAATGGTA GTCCCTCCAA ATGTCCTCGT TATATGAAAA TTCGTAACTG GGAGACAAAC 
ACTTCACTTA ATGACACGCT GCACTGCAAG GCTTCCATGC CAACACCCTG CACACAACAG 
ATATGCATGG GGGCAGTGAT GACGCCACCA CATTATGTTC GCAAACCAGA GGACGTCCGG 
ACCAAGGAGG AGCTTCTTCC CCTTGCTAAG GAATTCATTG ACCAATATTA CTCTTCAATG 
AAAAGATTTG GCTCCAAGGC ACACATGGAC AGACTACAAG AAGTAACAAA GGAGATTGAA 
GCTACAGATA CTTATCAGCT ACGAGATACA GAGCTTATTT ATGGAGCCAA GCATGCCTGG 
AGGAATGCAT CCCGATGTGT GGGGAGAATC CAGTGGTCAA AACTGCAGGT ATTTGATGCT 
CGAGACTGCA CCACTGCTCA TGGGATGTTC AATTACATTT GCAATCACAT TAAATATGCC 
ACCAACAAAG GCAACCTCAG ATCAGCAATC ACTATCTTCC CTCAAAGAAC GGATGGGAAG 
CGTGACTTTA GAATCTGGAA TGCTCAGCTT ATTCGCTATG CAGGATACAA ACAGCCTGAT 
GGCTCAGTGC TGGGAGACCC TGCAAATGTA GAATTGACAG AGATCTGCAT TCAGCAGGGC 
TGGAAGGCCC CTTATGGACG TTTTGATGTA CTACCTCTAC TGCTCCAAGC TGATGGCAAT 
GACCCTGAAT TGTTTGAGAT CCCCCCTGAA CTGATCATGG AAGTCAACAT TAGGCACCCC 
AGGCTAGAGT GGTTTAAAGA CCTGGCTTTG AAGTGGTATG GGCTGCCTGC TGTATCCAAT 
TTGCTGCTAG AAGTTGGAGG CTTGGAATTC AGTGCCTGTC CCTTCAGCGG ATGGTACATG 
GGGACAGAAA TTGGTGTTCG AGATTACTGT GACAACTCCC GCTACAACAT ACTGGAGGAA 
GTAGCCCTGA AAATGAATCT GGACACAAGG AAAACATCCT CTTTGTGGAA GGATCAAGCT 
CTGGTGGAGA TAAACATTGC TGTATTGTAC AGTTTCCAGA GTGATAAAAT AACAATCGTC 
GACCATCACT CAGCTACTGA GTCTTTCATC AAACATATGG AGAATGAATA CCGCTGCCGA 
GGAGGCTGTC CTGCAGACTG GGTTTGGATA GTACCGCCGA TGTCTGGAAG CATTACCCCT 
GTATTTCACC AGGAAATGTT AAACTACCGT CTCACACCTT CATTTGAGTA CCAGCCTGAT 
CCATGGAATA CCCACATATG GAAAGGGGTT AATGGAACAC CAACAAAGAA AAGAGCCATT 
GGATTTAAAA AGCTGGCAAA GGCAGTTAAA TTTTCAGCTA AATTAATGGG ACAGGCCATG 
GCTAAAAGAG TAAAGGCTAC AATTCTCTAC GCAACAGAAA CTGGGAAATC CGAAGTCTAT 
GCAAAAACTT TATGTGAAAT CTTCAAGCAT GCATTTGATG CAAAGGTTAT GTCAATGGAT 
GACTATGACA TTGTGCACCT AGAGCATGAA ACACTTGTCT TAGTAGTGAC TAGTACTTTT 
GGTAATGGTG ATCCACCAGA AAATGGGGAG AAGTTTGGCT GTGCATTAAT GGAAATGAGG 
CATCCCAACT CCAACCTGGA GGAGAGGACA AGCTACAAAG TCCGTTTTAA CAGCGTCTCA 
TCCTCTGCTG ATGCCCAGAA ATCGTCTGCT GATGAGACAG GACCCAGAGA CAACTTTGGT 
AGTGCAGGTC CCCTAGCTAA TGTTAGATTC TCAGCGTTTG GACTTGGCTC CCGAGCTTAC 
CCCCACTTCT GTGCCTTTGC CCGTGCGGTT GACACCCTGC TGGAGGAGCT TGGAGGAGAA 
AGAATACTGA AAATGGGGGA AGGGGATGAA TTGTGCGGTC AGGAAGAATC CTTTAGAACA 
TGGGCCAAAA AAGTGTTTCA GGCTGCATGT GACGTCTTTT GTGTAGGAGA TGATGTAAGT 
ATTGAAAAAG CAAACAACTC ACTGATTAGC AATGACCGCA GCTGGAAAAG AAGCAAGTAC 
CGGATCAGCT ATGTGGCAGA GGCACCAGAA CTTACACAAG CATTATACAG TATTCACAAG 
AAGAAAGTGT ACGGAGCTCG ATTGTTGTCT CGCCAGAACT TGCAAAGCCC AAAATCCAGC 
CGATCCACTA TATTCCTTCA ACTTCACACT AACGAACATA AAGAACTGTG CTACAAACCT 
GGAGACCATT TGGGAATCTT TCCTGGAAAC CATGAAGATC TAGTGAATGC CTTGATAGAG 
CAATTAGAAG ATGCACCTCC TGTAAACCAG CTGGTGAGGG TGGAGATGCT GGAAGAAAGA 
AATACTGCTT TAGGTGTAAT TAGTAACTGG ACAGAGGAGC AGCGAATCCC ACCCTGCACC 
ATCTTTCAAG CATTTAAGTA TTTCTTAGAT ATCACCACAC CCCCTACCCC TCTCCTCATG 
CAGCAAATTG CGCTGCTGGC AACTGATGAG AAAGAAAAGA ATCGGCTGGA GATCTTAAGC 
AAGGGCTTGC AGGAATATGA GCAATGGAAG TGGTACAAGA ATCCTACTAT TGTTGAGGTG 
CTAGAAGAGT TTCCTTCAAT TCAGATGCCT TCATCCCTGC TGCTTACACA ACTACCCTTG 
CTACAGCCTC GATACTACTC CATCAGCTCA TCACCTGAGA TGTATCCGGA AGAAGTGCAC 
CTTACTGTTG CTGTTGTGAC CTACCGGACA AGAGATGGAG ATGGGCCTAT ACATCATGGA 
GTCTGCTCCT CATGGCTCAA CAGAATACAG CCAGATGAAG TTGTGCCCTG CTTTGTCAGA 
GGAGCACCAA GTTTCCAGAT GCCAGAAGAC CCACAGGTCC CCTGCATTCT GATAGGACCA 
GGCACTGGCA TTGCTCCATT TAGGAGTTTT TGGCAGCAAC GTCTCTATGA TATGCAGCAC 
AGAGGACTAA AACCTTGTCC AATGATCTTG GTATTTGGGT GTAGGGAATC CAAAATTGAC 
CACATCTACA AAGAGGAAAC CATGCATGCA ATGAATAAAG GAGTGTTCAA AGAACTCTTT 
ACAGCTTATT CTCGGGAACC TAATAAACCA AAGAAATACG TTCAGGATGT ACTGAGGGAA 
CAGTTATCTG ATGTGACATA CAAAGCTCTG AAGGATCAAG GGGGACACAT ATATATTTGT 
GGTGATGTCA ACATGGCTGG GGATGTCCTG AAGAGTCTTC AGCATATTGT GAAAGAGCGT 
GGAAATCTGA CAGCTGAAGA AGCTGGGGCC TTCATCAGCA AACTAAGAGA TGACAATCGG 
TACCATGAAG ACATTTTTGG AGTCACTCTT AGGACGTATG AAGTTACAAA TCGTCTGAGA 
TCTGAATCTA TTGCATTTAT TGAGGAAAGC AAAAAGGACT CAGATGAGGT TTTCTGCTCA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004910558.1
CDS	500..4762
Translation	MEEYEFSVKQLQPNVISVRLFKRKVGGLGFLVKQRRNKPPVIISDLIRGGAAEQSGLVQVGDIILAVNDRPLVDASYESALEILRSISTETFVVLILRGPEGFTTHLETTFSGDGTPKTIRVTRPLCSKSKSTDLTSQSLYSKDHQNMVDSATSSAGSSWQEVHQLLNLNGLDGGKGDDISISPCLNGGEKANDILKEIEPVVSLLNAGMDLNGDGHRNVKQKDAEVQVESNSQTQPSTQKDQVNGIWKNNNKMPVVLNNPYLECEQVTLTGRHSPAKSQVNGSPSKCPRYMKIRNWETNTSLNDTLHCKASMPTPCTQQICMGAVMTPPHYVRKPEDVRTKEELLPLAKEFIDQYYSSMKRFGSKAHMDRLQEVTKEIEATDTYQLRDTELIYGAKHAWRNASRCVGRIQWSKLQVFDARDCTTAHGMFNYICNHIKYATNKGNLRSAITIFPQRTDGKRDFRIWNAQLIRYAGYKQPDGSVLGDPANVELTEICIQQGWKAPYGRFDVLPLLLQADGNDPELFEIPPELIMEVNIRHPRLEWFKDLALKWYGLPAVSNLLLEVGGLEFSACPFSGWYMGTEIGVRDYCDNSRYNILEEVALKMNLDTRKTSSLWKDQALVEINIAVLYSFQSDKITIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMLNYRLTPSFEYQPDPWNTHIWKGVNGTPTKKRAIGFKKLAKAVKFSAKLMGQAMAKRVKATILYATETGKSEVYAKTLCEIFKHAFDAKVMSMDDYDIVHLEHETLVLVVTSTFGNGDPPENGEKFGCALMEMRHPNSNLEERTSYKVRFNSVSSSADAQKSSADETGPRDNFGSAGPLANVRFSAFGLGSRAYPHFCAFARAVDTLLEELGGERILKMGEGDELCGQEESFRTWAKKVFQAACDVFCVGDDVSIEKANNSLISNDRSWKRSKYRISYVAEAPELTQALYSIHKKKVYGARLLSRQNLQSPKSSRSTIFLQLHTNEHKELCYKPGDHLGIFPGNHEDLVNALIEQLEDAPPVNQLVRVEMLEERNTALGVISNWTEEQRIPPCTIFQAFKYFLDITTPPTPLLMQQIALLATDEKEKNRLEILSKGLQEYEQWKWYKNPTIVEVLEEFPSIQMPSSLLLTQLPLLQPRYYSISSSPEMYPEEVHLTVAVVTYRTRDGDGPIHHGVCSSWLNRIQPDEVVPCFVRGAPSFQMPEDPQVPCILIGPGTGIAPFRSFWQQRLYDMQHRGLKPCPMILVFGCRESKIDHIYKEETMHAMNKGVFKELFTAYSREPNKPKKYVQDVLREQLSDVTYKALKDQGGHIYICGDVNMAGDVLKSLQHIVKERGNLTAEEAGAFISKLRDDNRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDSDEVFCS

Target ORF information:

RefSeq Version	XM_004910501.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nitric oxide synthase 1 (nos1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004910501.3

ORF Insert Sequence:

ATGGAGGAAT ATGAATTCAG CGTTAAACAA CTGCAGCCGA ATGTTATATC AGTACGCCTC 
TTTAAACGCA AAGTGGGCGG GCTGGGATTC CTGGTTAAGC AAAGGAGGAA CAAACCACCT 
GTAATTATTT CTGATTTAAT TCGGGGAGGG GCAGCAGAGC AGAGTGGGTT GGTGCAAGTA 
GGGGATATTA TCTTAGCAGT GAATGATAGG CCCCTTGTAG ATGCCAGCTA TGAAAGTGCC 
CTGGAGATAC TGCGTAGCAT TTCCACAGAG ACTTTCGTTG TACTGATCCT TAGAGGTCCA 
GAAGGATTCA CAACCCACTT GGAGACCACC TTTTCTGGAG ATGGGACTCC CAAGACAATT 
CGGGTCACCA GACCTCTGTG TTCTAAGTCC AAGTCAACTG ACTTGACAAG TCAGAGCCTT 
TATAGCAAAG ATCACCAAAA TATGGTGGAC AGTGCCACAA GTTCAGCTGG CTCTTCATGG 
CAGGAGGTTC ATCAGCTTCT TAACTTAAAT GGACTTGATG GTGGTAAAGG TGATGACATC 
AGTATCAGCC CTTGCCTTAA TGGAGGAGAA AAAGCTAATG ACATATTGAA AGAAATTGAG 
CCAGTTGTGA GCCTTCTGAA TGCAGGAATG GACCTAAATG GGGATGGACA TCGAAACGTG 
AAGCAAAAGG ATGCGGAAGT TCAGGTTGAA AGCAACTCGC AGACACAACC AAGCACGCAG 
AAGGACCAAG TCAATGGTAT CTGGAAAAAC AACAACAAGA TGCCTGTGGT TTTAAACAAC 
CCATATCTGG AGTGTGAGCA GGTGACATTA ACTGGTAGAC ATTCCCCTGC AAAGAGCCAA 
GTGAATGGTA GTCCCTCCAA ATGTCCTCGT TATATGAAAA TTCGTAACTG GGAGACAAAC 
ACTTCACTTA ATGACACGCT GCACTGCAAG GCTTCCATGC CAACACCCTG CACACAACAG 
ATATGCATGG GGGCAGTGAT GACGCCACCA CATTATGTTC GCAAACCAGA GGACGTCCGG 
ACCAAGGAGG AGCTTCTTCC CCTTGCTAAG GAATTCATTG ACCAATATTA CTCTTCAATG 
AAAAGATTTG GCTCCAAGGC ACACATGGAC AGACTACAAG AAGTAACAAA GGAGATTGAA 
GCTACAGATA CTTATCAGCT ACGAGATACA GAGCTTATTT ATGGAGCCAA GCATGCCTGG 
AGGAATGCAT CCCGATGTGT GGGGAGAATC CAGTGGTCAA AACTGCAGGT ATTTGATGCT 
CGAGACTGCA CCACTGCTCA TGGGATGTTC AATTACATTT GCAATCACAT TAAATATGCC 
ACCAACAAAG GCAACCTCAG ATCAGCAATC ACTATCTTCC CTCAAAGAAC GGATGGGAAG 
CGTGACTTTA GAATCTGGAA TGCTCAGCTT ATTCGCTATG CAGGATACAA ACAGCCTGAT 
GGCTCAGTGC TGGGAGACCC TGCAAATGTA GAATTGACAG AGATCTGCAT TCAGCAGGGC 
TGGAAGGCCC CTTATGGACG TTTTGATGTA CTACCTCTAC TGCTCCAAGC TGATGGCAAT 
GACCCTGAAT TGTTTGAGAT CCCCCCTGAA CTGATCATGG AAGTCAACAT TAGGCACCCC 
AGGCTAGAGT GGTTTAAAGA CCTGGCTTTG AAGTGGTATG GGCTGCCTGC TGTATCCAAT 
TTGCTGCTAG AAGTTGGAGG CTTGGAATTC AGTGCCTGTC CCTTCAGCGG ATGGTACATG 
GGGACAGAAA TTGGTGTTCG AGATTACTGT GACAACTCCC GCTACAACAT ACTGGAGGAA 
GTAGCCCTGA AAATGAATCT GGACACAAGG AAAACATCCT CTTTGTGGAA GGATCAAGCT 
CTGGTGGAGA TAAACATTGC TGTATTGTAC AGTTTCCAGA GTGATAAAAT AACAATCGTC 
GACCATCACT CAGCTACTGA GTCTTTCATC AAACATATGG AGAATGAATA CCGCTGCCGA 
GGAGGCTGTC CTGCAGACTG GGTTTGGATA GTACCGCCGA TGTCTGGAAG CATTACCCCT 
GTATTTCACC AGGAAATGTT AAACTACCGT CTCACACCTT CATTTGAGTA CCAGCCTGAT 
CCATGGAATA CCCACATATG GAAAGGGGTT AATGGAACAC CAACAAAGAA AAGAGCCATT 
GGATTTAAAA AGCTGGCAAA GGCAGTTAAA TTTTCAGCTA AATTAATGGG ACAGGCCATG 
GCTAAAAGAG TAAAGGCTAC AATTCTCTAC GCAACAGAAA CTGGGAAATC CGAAGTCTAT 
GCAAAAACTT TATGTGAAAT CTTCAAGCAT GCATTTGATG CAAAGGTTAT GTCAATGGAT 
GACTATGACA TTGTGCACCT AGAGCATGAA ACACTTGTCT TAGTAGTGAC TAGTACTTTT 
GGTAATGGTG ATCCACCAGA AAATGGGGAG AAGTTTGGCT GTGCATTAAT GGAAATGAGG 
CATCCCAACT CCAACCTGGA GGAGAGGACA AGCTACAAAG TCCGTTTTAA CAGCGTCTCA 
TCCTCTGCTG ATGCCCAGAA ATCGTCTGCT GATGAGACAG GACCCAGAGA CAACTTTGGT 
AGTGCAGGTC CCCTAGCTAA TGTTAGATTC TCAGCGTTTG GACTTGGCTC CCGAGCTTAC 
CCCCACTTCT GTGCCTTTGC CCGTGCGGTT GACACCCTGC TGGAGGAGCT TGGAGGAGAA 
AGAATACTGA AAATGGGGGA AGGGGATGAA TTGTGCGGTC AGGAAGAATC CTTTAGAACA 
TGGGCCAAAA AAGTGTTTCA GGCTGCATGT GACGTCTTTT GTGTAGGAGA TGATGTAAGT 
ATTGAAAAAG CAAACAACTC ACTGATTAGC AATGACCGCA GCTGGAAAAG AAGCAAGTAC 
CGGATCAGCT ATGTGGCAGA GGCACCAGAA CTTACACAAG CATTATACAG TATTCACAAG 
AAGAAAGTGT ACGGAGCTCG ATTGTTGTCT CGCCAGAACT TGCAAAGCCC AAAATCCAGC 
CGATCCACTA TATTCCTTCA ACTTCACACT AACGAACATA AAGAACTGTG CTACAAACCT 
GGAGACCATT TGGGAATCTT TCCTGGAAAC CATGAAGATC TAGTGAATGC CTTGATAGAG 
CAATTAGAAG ATGCACCTCC TGTAAACCAG CTGGTGAGGG TGGAGATGCT GGAAGAAAGA 
AATACTGCTT TAGGTGTAAT TAGTAACTGG ACAGAGGAGC AGCGAATCCC ACCCTGCACC 
ATCTTTCAAG CATTTAAGTA TTTCTTAGAT ATCACCACAC CCCCTACCCC TCTCCTCATG 
CAGCAAATTG CGCTGCTGGC AACTGATGAG AAAGAAAAGA ATCGGCTGGA GATCTTAAGC 
AAGGGCTTGC AGGAATATGA GCAATGGAAG TGGTACAAGA ATCCTACTAT TGTTGAGGTG 
CTAGAAGAGT TTCCTTCAAT TCAGATGCCT TCATCCCTGC TGCTTACACA ACTACCCTTG 
CTACAGCCTC GATACTACTC CATCAGCTCA TCACCTGAGA TGTATCCGGA AGAAGTGCAC 
CTTACTGTTG CTGTTGTGAC CTACCGGACA AGAGATGGAG ATGGGCCTAT ACATCATGGA 
GTCTGCTCCT CATGGCTCAA CAGAATACAG CCAGATGAAG TTGTGCCCTG CTTTGTCAGA 
GGAGCACCAA GTTTCCAGAT GCCAGAAGAC CCACAGGTCC CCTGCATTCT GATAGGACCA 
GGCACTGGCA TTGCTCCATT TAGGAGTTTT TGGCAGCAAC GTCTCTATGA TATGCAGCAC 
AGAGGACTAA AACCTTGTCC AATGATCTTG GTATTTGGGT GTAGGGAATC CAAAATTGAC 
CACATCTACA AAGAGGAAAC CATGCATGCA ATGAATAAAG GAGTGTTCAA AGAACTCTTT 
ACAGCTTATT CTCGGGAACC TAATAAACCA AAGAAATACG TTCAGGATGT ACTGAGGGAA 
CAGTTATCTG ATGTGACATA CAAAGCTCTG AAGGATCAAG GGGGACACAT ATATATTTGT 
GGTGATGTCA ACATGGCTGG GGATGTCCTG AAGAGTCTTC AGCATATTGT GAAAGAGCGT 
GGAAATCTGA CAGCTGAAGA AGCTGGGGCC TTCATCAGCA AACTAAGAGA TGACAATCGG 
TACCATGAAG ACATTTTTGG AGTCACTCTT AGGACGTATG AAGTTACAAA TCGTCTGAGA 
TCTGAATCTA TTGCATTTAT TGAGGAAAGC AAAAAGGACT CAGATGAGGT TTTCTGCTCA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nos1 ( XM_012968764.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012824218.1 Xenopus tropicalis nitric oxide synthase 1 (nos1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OXa03510
Clone ID Related Accession (Same CDS sequence)	XM_012968764.2
Accession Version	XM_012968764.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4251bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nitric oxide synthase, brain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012968764.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAAT ATGAATTCAG CGTTAAACAA CTGCAGCCGA ATGTTATATC AGTACGCCTC 
TTTAAACGCA AAGTGGGCGG GCTGGGATTC CTGGTTAAGC AAAGGAGGAA CAAACCACCT 
GTAATTATTT CTGATTTAAT TCGGGGAGGG GCAGCAGAGC AGAGTGGGTT GGTGCAAGTA 
GGGGATATTA TCTTAGCAGT GAATGATAGG CCCCTTGTAG ATGCCAGCTA TGAAAGTGCC 
CTGGAGATAC TGCGTAGCAT TTCCACAGAG ACTTTCGTTG TACTGATCCT TAGAGGTCCA 
GAAGGATTCA CAACCCACTT GGAGACCACC TTTTCTGGAG ATGGGACTCC CAAGACAATT 
CGGGTCACCA GACCTCTGTG TTCTAAGTCC AAGTCAACTG ACTTGACAAG TCAGAGCCTT 
TATAGCAAAG ATCACCAAAA TATGGTGGAC AGTGCCACAA GTTCAGCTGG CTCTTCATGG 
CAGGAGGTTC ATCAGCTTCT TAACTTAAAT GGACTTGATG GTGGTAAAGG TGATGACATC 
AGTATCAGCC CTTGCCTTAA TGGAGGAGAA AAAGCTAATG ACATATTGAA AGAAATTGAG 
CCAGTTGTGA GCCTTCTGAA TGCAGGAATG GACCTAAATG GGGATGGACA TCGAAACGTG 
AAGCAAAAGG ATGCGGAAGT TCAGGTTGAA AGCAACTCGC AGACACAACC AAGCACGCAG 
AAGGACCAAG TCAATGGTAT CTGGAAAAAC AACAACAAGA TGCCTGTGGT TTTAAACAAC 
CCATATCTGG AGTGTGAGCA GGTGACATTA ACTGGTAGAC ATTCCCCTGC AAAGAGCCAA 
GTGAATGGTA GTCCCTCCAA ATGTCCTCGT TATATGAAAA TTCGTAACTG GGAGACAAAC 
ACTTCACTTA ATGACACGCT GCACTGCAAG GCTTCCATGC CAACACCCTG CACACAACAG 
ATATGCATGG GGGCAGTGAT GACGCCACCA CATTATGTTC GCAAACCAGA GGACGTCCGG 
ACCAAGGAGG AGCTTCTTCC CCTTGCTAAG GAATTCATTG ACCAATATTA CTCTTCAATG 
AAAAGATTTG GCTCCAAGGC ACACATGGAC AGACTACAAG AAGTAACAAA GGAGATTGAA 
GCTACAGATA CTTATCAGCT ACGAGATACA GAGCTTATTT ATGGAGCCAA GCATGCCTGG 
AGGAATGCAT CCCGATGTGT GGGGAGAATC CAGTGGTCAA AACTGCAGGT ATTTGATGCT 
CGAGACTGCA CCACTGCTCA TGGGATGTTC AATTACATTT GCAATCACAT TAAATATGCC 
ACCAACAAAG GCAACCTCAG ATCAGCAATC ACTATCTTCC CTCAAAGAAC GGATGGGAAG 
CGTGACTTTA GAATCTGGAA TGCTCAGCTT ATTCGCTATG CAGGATACAA ACAGCCTGAT 
GGCTCAGTGC TGGGAGACCC TGCAAATGTA GAATTGACAG AGATCTGCAT TCAGCAGGGC 
TGGAAGGCCC CTTATGGACG TTTTGATGTA CTACCTCTAC TGCTCCAAGC TGATGGCAAT 
GACCCTGAAT TGTTTGAGAT CCCCCCTGAA CTGATCATGG AAGTCAACAT TAGGCACCCC 
AGGCTAGAGT GGTTTAAAGA CCTGGCTTTG AAGTGGTATG GGCTGCCTGC TGTATCCAAT 
TTGCTGCTAG AAGTTGGAGG CTTGGAATTC AGTGCCTGTC CCTTCAGCGG ATGGTACATG 
GGGACAGAAA TTGGTGTTCG AGATTACTGT GACAACTCCC GCTACAACAT ACTGGAGGAA 
GTAGCCCTGA AAATGAATCT GGACACAAGG AAAACATCCT CTTTGTGGAA GGATCAAGCT 
CTGGTGGAGA TAAACATTGC TGTATTGTAC AGTTTCCAGA GTGATAAAAT AACAATCGTC 
GACCATCACT CAGCTACTGA GTCTTTCATC AAACATATGG AGAATGAATA CCGCTGCCGA 
GGAGGCTGTC CTGCAGACTG GGTTTGGATA GTACCGCCGA TGTCTGGAAG CATTACCCCT 
GTATTTCACC AGGAAATGTT AAACTACCGT CTCACACCTT CATTTGAGTA CCAGCCTGAT 
CCATGGAATA CCCACATATG GAAAGGGGTT AATGGAACAC CAACAAAGAA AAGAGCCATT 
GGATTTAAAA AGCTGGCAAA GGCAGTTAAA TTTTCAGCTA AATTAATGGG ACAGGCCATG 
GCTAAAAGAG TAAAGGCTAC AATTCTCTAC GCAACAGAAA CTGGGAAATC CGAAGTCTAT 
GCAAAAACTT TATGTGAAAT CTTCAAGCAT GCATTTGATG CAAAGGTTAT GTCAATGGAT 
GACTATGACA TTGTGCACCT AGAGCATGAA ACACTTGTCT TAGTAGTGAC TAGTACTTTT 
GGTAATGGTG ATCCACCAGA AAATGGGGAG AAGTTTGGCT GTGCATTAAT GGAAATGAGG 
CATCCCAACT CCAACCTGGA GGAGAGGACA AGCTACAAAG TCCGTTTTAA CAGCGTCTCA 
TCCTCTGCTG ATGCCCAGAA ATCGTCTGCT GATGAGACAG GACCCAGAGA CAACTTTGGT 
AGTGCAGGTC CCCTAGCTAA TGTTAGATTC TCAGCGTTTG GACTTGGCTC CCGAGCTTAC 
CCCCACTTCT GTGCCTTTGC CCGTGCGGTT GACACCCTGC TGGAGGAGCT TGGAGGAGAA 
AGAATACTGA AAATGGGGGA AGGGGATGAA TTGTGCGGTC AGGAAGAATC CTTTAGAACA 
TGGGCCAAAA AAGTGTTTCA GGCTGCATGT GACGTCTTTT GTGTAGGAGA TGATGTAAGT 
ATTGAAAAAG CAAACAACTC ACTGATTAGC AATGACCGCA GCTGGAAAAG AAGCAAGTAC 
CGGATCAGCT ATGTGGCAGA GGCACCAGAA CTTACACAAG CATTATACAG TATTCACAAG 
AAGAAAGTGT ACGGAGCTCG ATTGTTGTCT CGCCAGAACT TGCAAAGCCC AAAATCCAGC 
CGATCCACTA TATTCCTTCA ACTTCACACT AACGAACATA AAGAACTGTG CTACAAACCT 
GGAGACCATT TGGGAATCTT TCCTGGAAAC CATGAAGATC TAGTGAATGC CTTGATAGAG 
CAATTAGAAG ATGCACCTCC TGTAAACCAG CTGGTGAGGG TGGAGATGCT GGAAGAAAGA 
AATACTGCTT TAGGTGTAAT TAGTAACTGG ACAGAGGAGC AGCGAATCCC ACCCTGCACC 
ATCTTTCAAG CATTTAAGTA TTTCTTAGAT ATCACCACAC CCCCTACCCC TCTCCTCATG 
CAGCAAATTG CGCTGCTGGC AACTGATGAG AAAGAAAAGA ATCGGCTGGA GATCTTAAGC 
AAGGGCTTGC AGGAATATGA GCAATGGAAG TGGTACAAGA ATCCTACTAT TGTTGAGGTG 
CTAGAAGAGT TTCCTTCAAT TCAGATGCCT TCATCCCTGC TGCTTACACA ACTACCCTTG 
CTACAGCCTC GATACTACTC CATCAGCTCA TCACCTGAGA TGTATCCGGA AGAAGTGCAC 
CTTACTGTTG CTGTTGTGAC CTACCGGACA AGAGATGGAG ATGGGCCTAT ACATCATGGA 
GTCTGCTCCT CATGGCTCAA CAGAATACAG CCAGATGAAG TTGTGCCCTG CTTTGTCAGA 
GGAGCACCAA GTTTCCAGAT GCCAGAAGAC CCACAGGTCC CCTGCATTCT GATAGGACCA 
GGCACTGGCA TTGCTCCATT TAGGAGTTTT TGGCAGCAAC GTCTCTATGA TATGCAGCAC 
AGAGGACTAA AACCTTGTCC AATGATCTTG GTATTTGGGT GTAGGGAATC CAAAATTGAC 
CACATCTACA AAGAGGAAAC CATGCATGCA ATGAATAAAG GAGTGTTCAA AGAACTCTTT 
ACAGCTTATT CTCGGGAACC TAATAAACCA AAGAAATACG TTCAGGATGT ACTGAGGGAA 
CAGTTATCTG ATGTGACATA CAAAGCTCTG AAGGATCAAG GGGGACACAT ATATATTTGT 
GGTGATGTCA ACATGGCTGG GGATGTCCTG AAGAGTCTTC AGCATATTGT GAAAGAGCGT 
GGAAATCTGA CAGCTGAAGA AGCTGGGGCC TTCATCAGCA AACTAAGAGA TGACAATCGG 
TACCATGAAG ACATTTTTGG AGTCACTCTT AGGACGTATG AAGTTACAAA TCGTCTGAGA 
TCTGAATCTA TTGCATTTAT TGAGGAAAGC AAAAAGGACT CAGATGAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012824218.1
CDS	500..4750
Translation	MEEYEFSVKQLQPNVISVRLFKRKVGGLGFLVKQRRNKPPVIISDLIRGGAAEQSGLVQVGDIILAVNDRPLVDASYESALEILRSISTETFVVLILRGPEGFTTHLETTFSGDGTPKTIRVTRPLCSKSKSTDLTSQSLYSKDHQNMVDSATSSAGSSWQEVHQLLNLNGLDGGKGDDISISPCLNGGEKANDILKEIEPVVSLLNAGMDLNGDGHRNVKQKDAEVQVESNSQTQPSTQKDQVNGIWKNNNKMPVVLNNPYLECEQVTLTGRHSPAKSQVNGSPSKCPRYMKIRNWETNTSLNDTLHCKASMPTPCTQQICMGAVMTPPHYVRKPEDVRTKEELLPLAKEFIDQYYSSMKRFGSKAHMDRLQEVTKEIEATDTYQLRDTELIYGAKHAWRNASRCVGRIQWSKLQVFDARDCTTAHGMFNYICNHIKYATNKGNLRSAITIFPQRTDGKRDFRIWNAQLIRYAGYKQPDGSVLGDPANVELTEICIQQGWKAPYGRFDVLPLLLQADGNDPELFEIPPELIMEVNIRHPRLEWFKDLALKWYGLPAVSNLLLEVGGLEFSACPFSGWYMGTEIGVRDYCDNSRYNILEEVALKMNLDTRKTSSLWKDQALVEINIAVLYSFQSDKITIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMLNYRLTPSFEYQPDPWNTHIWKGVNGTPTKKRAIGFKKLAKAVKFSAKLMGQAMAKRVKATILYATETGKSEVYAKTLCEIFKHAFDAKVMSMDDYDIVHLEHETLVLVVTSTFGNGDPPENGEKFGCALMEMRHPNSNLEERTSYKVRFNSVSSSADAQKSSADETGPRDNFGSAGPLANVRFSAFGLGSRAYPHFCAFARAVDTLLEELGGERILKMGEGDELCGQEESFRTWAKKVFQAACDVFCVGDDVSIEKANNSLISNDRSWKRSKYRISYVAEAPELTQALYSIHKKKVYGARLLSRQNLQSPKSSRSTIFLQLHTNEHKELCYKPGDHLGIFPGNHEDLVNALIEQLEDAPPVNQLVRVEMLEERNTALGVISNWTEEQRIPPCTIFQAFKYFLDITTPPTPLLMQQIALLATDEKEKNRLEILSKGLQEYEQWKWYKNPTIVEVLEEFPSIQMPSSLLLTQLPLLQPRYYSISSSPEMYPEEVHLTVAVVTYRTRDGDGPIHHGVCSSWLNRIQPDEVVPCFVRGAPSFQMPEDPQVPCILIGPGTGIAPFRSFWQQRLYDMQHRGLKPCPMILVFGCRESKIDHIYKEETMHAMNKGVFKELFTAYSREPNKPKKYVQDVLREQLSDVTYKALKDQGGHIYICGDVNMAGDVLKSLQHIVKERGNLTAEEAGAFISKLRDDNRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDSDE

Target ORF information:

RefSeq Version	XM_012968764.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nitric oxide synthase 1 (nos1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012968764.2

ORF Insert Sequence:

ATGGAGGAAT ATGAATTCAG CGTTAAACAA CTGCAGCCGA ATGTTATATC AGTACGCCTC 
TTTAAACGCA AAGTGGGCGG GCTGGGATTC CTGGTTAAGC AAAGGAGGAA CAAACCACCT 
GTAATTATTT CTGATTTAAT TCGGGGAGGG GCAGCAGAGC AGAGTGGGTT GGTGCAAGTA 
GGGGATATTA TCTTAGCAGT GAATGATAGG CCCCTTGTAG ATGCCAGCTA TGAAAGTGCC 
CTGGAGATAC TGCGTAGCAT TTCCACAGAG ACTTTCGTTG TACTGATCCT TAGAGGTCCA 
GAAGGATTCA CAACCCACTT GGAGACCACC TTTTCTGGAG ATGGGACTCC CAAGACAATT 
CGGGTCACCA GACCTCTGTG TTCTAAGTCC AAGTCAACTG ACTTGACAAG TCAGAGCCTT 
TATAGCAAAG ATCACCAAAA TATGGTGGAC AGTGCCACAA GTTCAGCTGG CTCTTCATGG 
CAGGAGGTTC ATCAGCTTCT TAACTTAAAT GGACTTGATG GTGGTAAAGG TGATGACATC 
AGTATCAGCC CTTGCCTTAA TGGAGGAGAA AAAGCTAATG ACATATTGAA AGAAATTGAG 
CCAGTTGTGA GCCTTCTGAA TGCAGGAATG GACCTAAATG GGGATGGACA TCGAAACGTG 
AAGCAAAAGG ATGCGGAAGT TCAGGTTGAA AGCAACTCGC AGACACAACC AAGCACGCAG 
AAGGACCAAG TCAATGGTAT CTGGAAAAAC AACAACAAGA TGCCTGTGGT TTTAAACAAC 
CCATATCTGG AGTGTGAGCA GGTGACATTA ACTGGTAGAC ATTCCCCTGC AAAGAGCCAA 
GTGAATGGTA GTCCCTCCAA ATGTCCTCGT TATATGAAAA TTCGTAACTG GGAGACAAAC 
ACTTCACTTA ATGACACGCT GCACTGCAAG GCTTCCATGC CAACACCCTG CACACAACAG 
ATATGCATGG GGGCAGTGAT GACGCCACCA CATTATGTTC GCAAACCAGA GGACGTCCGG 
ACCAAGGAGG AGCTTCTTCC CCTTGCTAAG GAATTCATTG ACCAATATTA CTCTTCAATG 
AAAAGATTTG GCTCCAAGGC ACACATGGAC AGACTACAAG AAGTAACAAA GGAGATTGAA 
GCTACAGATA CTTATCAGCT ACGAGATACA GAGCTTATTT ATGGAGCCAA GCATGCCTGG 
AGGAATGCAT CCCGATGTGT GGGGAGAATC CAGTGGTCAA AACTGCAGGT ATTTGATGCT 
CGAGACTGCA CCACTGCTCA TGGGATGTTC AATTACATTT GCAATCACAT TAAATATGCC 
ACCAACAAAG GCAACCTCAG ATCAGCAATC ACTATCTTCC CTCAAAGAAC GGATGGGAAG 
CGTGACTTTA GAATCTGGAA TGCTCAGCTT ATTCGCTATG CAGGATACAA ACAGCCTGAT 
GGCTCAGTGC TGGGAGACCC TGCAAATGTA GAATTGACAG AGATCTGCAT TCAGCAGGGC 
TGGAAGGCCC CTTATGGACG TTTTGATGTA CTACCTCTAC TGCTCCAAGC TGATGGCAAT 
GACCCTGAAT TGTTTGAGAT CCCCCCTGAA CTGATCATGG AAGTCAACAT TAGGCACCCC 
AGGCTAGAGT GGTTTAAAGA CCTGGCTTTG AAGTGGTATG GGCTGCCTGC TGTATCCAAT 
TTGCTGCTAG AAGTTGGAGG CTTGGAATTC AGTGCCTGTC CCTTCAGCGG ATGGTACATG 
GGGACAGAAA TTGGTGTTCG AGATTACTGT GACAACTCCC GCTACAACAT ACTGGAGGAA 
GTAGCCCTGA AAATGAATCT GGACACAAGG AAAACATCCT CTTTGTGGAA GGATCAAGCT 
CTGGTGGAGA TAAACATTGC TGTATTGTAC AGTTTCCAGA GTGATAAAAT AACAATCGTC 
GACCATCACT CAGCTACTGA GTCTTTCATC AAACATATGG AGAATGAATA CCGCTGCCGA 
GGAGGCTGTC CTGCAGACTG GGTTTGGATA GTACCGCCGA TGTCTGGAAG CATTACCCCT 
GTATTTCACC AGGAAATGTT AAACTACCGT CTCACACCTT CATTTGAGTA CCAGCCTGAT 
CCATGGAATA CCCACATATG GAAAGGGGTT AATGGAACAC CAACAAAGAA AAGAGCCATT 
GGATTTAAAA AGCTGGCAAA GGCAGTTAAA TTTTCAGCTA AATTAATGGG ACAGGCCATG 
GCTAAAAGAG TAAAGGCTAC AATTCTCTAC GCAACAGAAA CTGGGAAATC CGAAGTCTAT 
GCAAAAACTT TATGTGAAAT CTTCAAGCAT GCATTTGATG CAAAGGTTAT GTCAATGGAT 
GACTATGACA TTGTGCACCT AGAGCATGAA ACACTTGTCT TAGTAGTGAC TAGTACTTTT 
GGTAATGGTG ATCCACCAGA AAATGGGGAG AAGTTTGGCT GTGCATTAAT GGAAATGAGG 
CATCCCAACT CCAACCTGGA GGAGAGGACA AGCTACAAAG TCCGTTTTAA CAGCGTCTCA 
TCCTCTGCTG ATGCCCAGAA ATCGTCTGCT GATGAGACAG GACCCAGAGA CAACTTTGGT 
AGTGCAGGTC CCCTAGCTAA TGTTAGATTC TCAGCGTTTG GACTTGGCTC CCGAGCTTAC 
CCCCACTTCT GTGCCTTTGC CCGTGCGGTT GACACCCTGC TGGAGGAGCT TGGAGGAGAA 
AGAATACTGA AAATGGGGGA AGGGGATGAA TTGTGCGGTC AGGAAGAATC CTTTAGAACA 
TGGGCCAAAA AAGTGTTTCA GGCTGCATGT GACGTCTTTT GTGTAGGAGA TGATGTAAGT 
ATTGAAAAAG CAAACAACTC ACTGATTAGC AATGACCGCA GCTGGAAAAG AAGCAAGTAC 
CGGATCAGCT ATGTGGCAGA GGCACCAGAA CTTACACAAG CATTATACAG TATTCACAAG 
AAGAAAGTGT ACGGAGCTCG ATTGTTGTCT CGCCAGAACT TGCAAAGCCC AAAATCCAGC 
CGATCCACTA TATTCCTTCA ACTTCACACT AACGAACATA AAGAACTGTG CTACAAACCT 
GGAGACCATT TGGGAATCTT TCCTGGAAAC CATGAAGATC TAGTGAATGC CTTGATAGAG 
CAATTAGAAG ATGCACCTCC TGTAAACCAG CTGGTGAGGG TGGAGATGCT GGAAGAAAGA 
AATACTGCTT TAGGTGTAAT TAGTAACTGG ACAGAGGAGC AGCGAATCCC ACCCTGCACC 
ATCTTTCAAG CATTTAAGTA TTTCTTAGAT ATCACCACAC CCCCTACCCC TCTCCTCATG 
CAGCAAATTG CGCTGCTGGC AACTGATGAG AAAGAAAAGA ATCGGCTGGA GATCTTAAGC 
AAGGGCTTGC AGGAATATGA GCAATGGAAG TGGTACAAGA ATCCTACTAT TGTTGAGGTG 
CTAGAAGAGT TTCCTTCAAT TCAGATGCCT TCATCCCTGC TGCTTACACA ACTACCCTTG 
CTACAGCCTC GATACTACTC CATCAGCTCA TCACCTGAGA TGTATCCGGA AGAAGTGCAC 
CTTACTGTTG CTGTTGTGAC CTACCGGACA AGAGATGGAG ATGGGCCTAT ACATCATGGA 
GTCTGCTCCT CATGGCTCAA CAGAATACAG CCAGATGAAG TTGTGCCCTG CTTTGTCAGA 
GGAGCACCAA GTTTCCAGAT GCCAGAAGAC CCACAGGTCC CCTGCATTCT GATAGGACCA 
GGCACTGGCA TTGCTCCATT TAGGAGTTTT TGGCAGCAAC GTCTCTATGA TATGCAGCAC 
AGAGGACTAA AACCTTGTCC AATGATCTTG GTATTTGGGT GTAGGGAATC CAAAATTGAC 
CACATCTACA AAGAGGAAAC CATGCATGCA ATGAATAAAG GAGTGTTCAA AGAACTCTTT 
ACAGCTTATT CTCGGGAACC TAATAAACCA AAGAAATACG TTCAGGATGT ACTGAGGGAA 
CAGTTATCTG ATGTGACATA CAAAGCTCTG AAGGATCAAG GGGGACACAT ATATATTTGT 
GGTGATGTCA ACATGGCTGG GGATGTCCTG AAGAGTCTTC AGCATATTGT GAAAGAGCGT 
GGAAATCTGA CAGCTGAAGA AGCTGGGGCC TTCATCAGCA AACTAAGAGA TGACAATCGG 
TACCATGAAG ACATTTTTGG AGTCACTCTT AGGACGTATG AAGTTACAAA TCGTCTGAGA 
TCTGAATCTA TTGCATTTAT TGAGGAAAGC AAAAAGGACT CAGATGAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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nos1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

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