RASAL2 gene cDNA ORF clone, Gallus gallus(chicken)

mRNA	Protein	Name
XM_004943254.2	XP_004943311.2	ras GTPase-activating protein nGAP isoform X11
XM_015290513.1	XP_015145999.1	ras GTPase-activating protein nGAP isoform X6
XM_004943250.2	XP_004943307.1	ras GTPase-activating protein nGAP isoform X7
XM_015290514.1	XP_015146000.1	ras GTPase-activating protein nGAP isoform X8
XM_015290512.2	XP_015145998.1	ras GTPase-activating protein nGAP isoform X5
XM_015290514.2	XP_015146000.1	ras GTPase-activating protein nGAP isoform X8
XM_015290516.1	XP_015146002.1	ras GTPase-activating protein nGAP isoform X10
XM_015290510.1	XP_015145996.1	ras GTPase-activating protein nGAP isoform X1
XM_004943245.2	XP_004943302.2	ras GTPase-activating protein nGAP isoform X2
XM_015290512.1	XP_015145998.1	ras GTPase-activating protein nGAP isoform X5
XM_015290515.1	XP_015146001.1	ras GTPase-activating protein nGAP isoform X9
XM_015290520.1	XP_015146006.1	ras GTPase-activating protein nGAP isoform X13
XM_015290515.2	XP_015146001.1	ras GTPase-activating protein nGAP isoform X9
XM_015290520.2	XP_015146006.1	ras GTPase-activating protein nGAP isoform X13
XM_015290519.1	XP_015146005.1	ras GTPase-activating protein nGAP isoform X12
XM_015290511.2	XP_015145997.1	ras GTPase-activating protein nGAP isoform X3
XM_015290516.2	XP_015146002.1	ras GTPase-activating protein nGAP isoform X10
XM_004943246.2	XP_004943303.2	ras GTPase-activating protein nGAP isoform X4
XM_004943245.3	XP_004943302.2	ras GTPase-activating protein nGAP isoform X2
XM_004943246.3	XP_004943303.2	ras GTPase-activating protein nGAP isoform X4
XM_004943250.3	XP_004943307.1	ras GTPase-activating protein nGAP isoform X7
XM_015290511.1	XP_015145997.1	ras GTPase-activating protein nGAP isoform X3
XM_015290510.2	XP_015145996.1	ras GTPase-activating protein nGAP isoform X1
XM_015290513.2	XP_015145999.1	ras GTPase-activating protein nGAP isoform X6
XM_004943254.3	XP_004943311.2	ras GTPase-activating protein nGAP isoform X11
XM_015290519.2	XP_015146005.1	ras GTPase-activating protein nGAP isoform X12

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa152361	XM_015290510.2 Latest version!	Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OGa152362	XM_004943245.3 Latest version!	Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OGa152363	XM_015290511.2 Latest version!	Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OGa152364	XM_004943246.3 Latest version!	Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OGa152365	XM_015290512.2 Latest version!	Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OGa152366	XM_015290513.2 Latest version!	Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OGa152367	XM_015290514.2 Latest version!	Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OGa152368	XM_015290515.2 Latest version!	Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OGa152369	XM_015290516.2 Latest version!	Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OGa152370	XM_004943254.3 Latest version!	Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OGa152371	XM_015290519.2 Latest version!	Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OGa152372	XM_015290520.2 Latest version!	Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OGa39039	XM_015290510.1 Latest version!	Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OGa39040	XM_004943245.2 Latest version!	Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OGa39041	XM_015290511.1 Latest version!	Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OGa39042	XM_004943246.2 Latest version!	Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OGa39043	XM_015290512.1 Latest version!	Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OGa39047	XM_015290515.1 Latest version!	Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OGa39048	XM_015290516.1 Latest version!	Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OGa39049	XM_004943254.2 Latest version!	Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OGa39050	XM_015290519.1 Latest version!	Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OGa39051	XM_015290520.1 Latest version!	Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OGa11742	XM_004943250.3 Latest version!	Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OGa39044	XM_015290513.1 Latest version!	Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OGa39045	XM_004943250.2 Latest version!	Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OGa39046	XM_015290514.1 Latest version!	Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00

RASAL2 ( XM_015290510.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015145996.1 Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa152361
Clone ID Related Accession (Same CDS sequence)	XM_015290510.2
Accession Version	XM_015290510.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	ras GTPase-activating protein nGAP isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006095.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015290510.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGCTT TTAAAAAGTA TTACAGTCTC TCTCCCAGGA CCTTTAGGAG GTCTTTGTCG 
GAGCAAGATG CTGGCACCGC TGCCTTAAAG GACTATAATT GGTACCACCA AATGAGAAGC 
AAGAAGGGGA GCAAACAAAC AGCTTTGGAC CGAGAGAAAG CACTCGGAGC GTACAGGTGG 
CAAACACATC CCCGTCTTCC CTGGAGGACA AGCAGAAGAG CACACTCTTG CAGCCTCAGA 
GGGGAAGGTA ATGATTCGAG TCTTGTCAAA AGTTCTGCAA GCATGGATGC TACCAGCGCC 
GACGATCTCC ACCAAGACTC AACGGAGATG CTCTTCCAGG CAAGGACCAG ATCACCGCGG 
GTTCCCTTCA ACCGCTCCCT GTCTGAACCT GTGCCGCACT CTGCAAACAG GAATGCCAAG 
CCCTTTCTGC AGGCGGTGCG CTCTGTGGAT TGTGAGCAGC ACAGCTATTT TGTCCGTGGC 
ATCTTCTCAA CTCTCTCAAG TGGCTTTTCA AAGATAATAA ATCGAAGGGG TGAGCAGCCC 
CCCACTGAGC CACTGGAGGA GTGGAAACCA GCCGGTAGTC AAATTGACCC GTGCCCAGGT 
AATGGACTCT GTTCTGTTTG TGTTACAGAT GTAAAAGGAC CACCTACGCA CAGGCTGTCC 
TGCGGCCAGT CCCCCTACAC CGAGACGACA ACATGGGAGA GGAAGTACTG CATCCTGACT 
GACAGCCAGC TGGTGCTGCT CAACAGGGAG AAGGAGGTGC CCACCGAGGG GTTGCAGGAG 
CCGCAGGATG CAACCAAAGG CCGCTGTCTG CGCCGCACGG TGAGCGTGCC CTCCGAGGGC 
CAGTTCCCCG AGTACCCACC AGATGGGGCC GCCAAGCTGG AGGTCCCGGC AGAGAGATCT 
CCTCGCAGAC GGAGCATCTC TGGGACCAGC ACATCTGAGA AAACCAACTC CATGGATGCT 
GCAAATACCT CTCCTTTCAA AGTATCGGGC TTCTTCAGTA AACGTCTGAA AGGGTCCATC 
AAGAGGACAA AGAGTCAGTC AAAGCTGGAC AGGAACACAA GTTTCCGCCT CCCATCCCTC 
CGCAATGCTG ATGACAGGTC ACGTGGGCTG CCGAAGCTGA AGGAGTCCCG CTCTCATGAG 
TCCCTGCTGA GCCCCGGCAG CGCAGTGGAA GCCCTGGACC TTGGGGCAGA AGAAAAGGTC 
TTTGTAAAGC CACTGCACAG CAGCATCCTC GGACAGGACT TCTGCTTTGA GGTAACCTAC 
TCCAGCGGCA GTAAATGCTT CAGCTGTTCC TCTGCTGCAG AGAGGGATAA GTGGATGGAA 
AACCTACGCA GAACAGTGCA GCCGAATAAG GACAACTGCC GTCGAGCTGA AAATGTGCTC 
CGTCTCTGGA TCATTGAGGC AAAGGACCTG GCCCCCAAGA AGAAATACTT CTGTGAGCTG 
TGCCTTGATG ACACTCTCTT TGCCCGCACC ACCAGCAAAA CGAAAGCAGA TAACATTTTC 
TGGGGGGAGC ACTTTGAGTT CTACGGCCTC CCAGCTCTTC ACAGCATCAC GGTTCACATC 
TACAAGGATG TGGAGAAGAA AAAGAAAAAG GACAAAAACA ATTACGTCGG CCTGGTCAAC 
ATTCCCATGG CCACTGTAAC GGGGCGCCAG TTTGTGGAAA AATGGTACCC AGTCAGCACA 
CCTACACCCA ACAAAGGGAA ATCAGGAGGC CCTTCCATTC GCATCAAGTC GCGTTATCAG 
ACCATCACCA TCTTACCCAT GGAGCAATAC AAGGAGTTTG CAGAGTTTGT TACCAGCAAC 
TACACGATGC TGTGCTCTGT GCTGGAGCCA GTCATCAGCG TAAGGAATAA GGAAGAGATG 
GCTTGTGCAA TGGTGCACAT TCTTCAGAGC ACGGGGAGAG CCAAGGACTT CCTAACGGAT 
CTGGTGATGT CTGAGGTGGA TCGCTGCGGG GAACACGATG TCTTGATCTT CAGGGAGAAC 
ACACTTGCTA CCAAAGCTAT TGAGGAATAC CTGAAGTTGG TAGGGCAGAA GTATCTCCAT 
GATGCGCTAG GGGAGTTTAT CAAGGCTTTG TATGAGTCAG ATGAAAACTG TGAAGTGGAC 
CCCAGCAAAT GTTCATCCAG TGAATTAACA GACCATCAGA GCAACCTGAA AATGTGTTGT 
GAGCTGGCCT TCTGCAAGAT TATCAATTCC TACTGCGTGT TCCCTCGTGA GCTGAAGGAG 
GTGTTTGCAT CTTGGAAGCA GCAGTGCCTG AGCAGGGGCA AGCAGGACAT CAGCGAGCGG 
CTGATCAGTG CCTCCCTGTT CCTCCGCTTC CTGTGCCCCG CCATCATGTC CCCCAGCCTC 
TTCAGCCTCA TGCAGGAGTA TCCTGACGAC CGGACATCGC GCACGCTCAC ACTCATTGCT 
AAAGTCATTC AGAATCTCGC CAATTTTGCT AAGTTTGGTA ACAAGGAAGA GTACATGGCT 
TTCATGAACG ACTTTCTGGA ACATGAGTGG GGGGGAATGA AGCGATTTCT CCTGGAGATC 
TCTAATCCAG ATACCATCTC AAACATGCCT GGATTTGAGG GCTATATCGA CCTGGGCAGA 
GAGCTCTCAG TTTTGCATTC CCTCTTATGG GAGGTTGTGT CCCAACTTGA TAAGGGTGAA 
AATTCCTTCC TACAGGCGAC CGTGGCAAAA CTGGGACCTC TTCCTCGTAT TCTTGCCGAC 
ATCACCAAAT CAATGACCAA CCCTACGCCA ATACAGCAGC AGCTGAGGCG TTTCACTGAG 
CACAGCTCTA GTCCAAACGT CAGTGGCAGT CTCTCCTCAG GGCTTCAGAA GATATTCGAG 
GACCCCACTG ATGGTGACTT GCATAAACTG AAGTCTCCAA CCCAGGAAAA CATAGATGGC 
TATTTCAGGG GCAAGACCTT GCTGTTGGTT CAGCAGGCGT CCACCCAGAG CATGACTTAC 
TCAGATAAGG ACGAAAGAGA GAGCATCTTG CCCAACGGAC GTAGCATCTC CCTCATGGAC 
CTGCAGGATC CCCATACCGC TCACAGCGAC CACGCTTCCA TGATGCACGA CGTGCCTTTG 
CGCTTGGCCG GCAGCCAGCT CTCCATCACC CAGGTGGCCA ACATCAAGCA GCTGTGGGAG 
ACCCAGAGCA CGCCCCAAAG CGCTCCCCAG GTGAGGAGGC CGCTGCACCC GGCTTTGAAC 
CAGCAGGGCA GCCTCCAGCC TCTGTCATTC CAGAACCCGG TGTACCACCT CAACAACCCA 
CCTGCGCCGT TGCCCAAGGC CTCCGTGGAC TCCAGCCTGG AGAACCTGAG CACCGCCAGC 
TCCCAGAGCC GAAGCAACAG CGAAGACTTC AAGCTCAGCG GGCCCAGCAA CAGCAGCATG 
GAGGACTTCA CCAAGCGCAG CACGCAGAGC GAGGACTTCT CTAGGCGGCA CACGGTCCCG 
GACAAGCACG TCCCCATCGC GTTGCCCCGC CAGAACAGCA GCAGCCAGGC ACAGATCCGC 
AAAATGGACC AGGCCGGGTT AGGGGCCAGG GCCAAAGCGC CGCAGTCGCT GCCGCACAGC 
GCGTCCCTGC GCAGCACGGG CAGCATGTCG GCCGTGTCGG GGGCCGTGGT GACTGAGCCC 
GTCCAGAACG GCAGCCGCTC CCGGCAGCAG TCGTCCTCCT CCAGGGAGAG CCCTGTGCCG 
AAGGTGAGGG CCATTCAGAG GCAGCAAACG CAGCAGGTCC AGTCTCCTGT GGACTCAGCC 
ACAATGTCAC CAGTGGAAAG GACAGCAGCG TGGGTTCTCA ATAACGGGCA GTACGAGGAG 
GTCGAGGAGG ACACAGAGCA GAACCCAGAT GAAGCCAAGC ATGCTGAGAA GTACGAACAA 
GAGATAGCAA AGCTGAAGGA ACGCTTGAAA GTGTCGAGCC GCCGGCTGGA GGAGTACGAG 
CGGCGGCTCC TGGTGCAGGA GCAGCAAATG CAGAAGCTGC TGATGGAGTA CAAGTCCAGG 
CTGGAGGACA GCGAGGAGAG ACTGCGCAGG CAACAGGAGG AGAAGGACAG CCAGATGAAA 
AGCATTATCA GCAGGCTCAT GGCAGTTGAA GAGGAACTGA AGAAAGATCA TGCAGAGATG 
CAAGCTGTCA TCGATGCAAA GCAGAAAATT ATTGATGCAC AGGTCATTAA TGGGAATGAG 
ATAATTCAAA CAGGAGAAGC GGATCGTGTC CCTGGACTCT GCCAACACGC GGCTGATGAG 
TGCCCTGACG CAGGTGAAGG AGCGCTACAG CATGCAAGTC CGCAATGGCA TCTCCCCCAC 
CAACCCCACC AAGCTTTCCA TCACGGAGAA TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015145996.1
CDS	637..4932
Translation	MSAFKKYYSLSPRTFRRSLSEQDAGTAALKDYNWYHQMRSKKGSKQTALDREKALGAYRWQTHPRLPWRTSRRAHSCSLRGEGNDSSLVKSSASMDATSADDLHQDSTEMLFQARTRSPRVPFNRSLSEPVPHSANRNAKPFLQAVRSVDCEQHSYFVRGIFSTLSSGFSKIINRRGEQPPTEPLEEWKPAGSQIDPCPGNGLCSVCVTDVKGPPTHRLSCGQSPYTETTTWERKYCILTDSQLVLLNREKEVPTEGLQEPQDATKGRCLRRTVSVPSEGQFPEYPPDGAAKLEVPAERSPRRRSISGTSTSEKTNSMDAANTSPFKVSGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNADDRSRGLPKLKESRSHESLLSPGSAVEALDLGAEEKVFVKPLHSSILGQDFCFEVTYSSGSKCFSCSSAAERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYGLPALHSITVHIYKDVEKKKKKDKNNYVGLVNIPMATVTGRQFVEKWYPVSTPTPNKGKSGGPSIRIKSRYQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEEMACAMVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTLATKAIEEYLKLVGQKYLHDALGEFIKALYESDENCEVDPSKCSSSELTDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCLSRGKQDISERLISASLFLRFLCPAIMSPSLFSLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNMPGFEGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVAKLGPLPRILADITKSMTNPTPIQQQLRRFTEHSSSPNVSGSLSSGLQKIFEDPTDGDLHKLKSPTQENIDGYFRGKTLLLVQQASTQSMTYSDKDERESILPNGRSISLMDLQDPHTAHSDHASMMHDVPLRLAGSQLSITQVANIKQLWETQSTPQSAPQVRRPLHPALNQQGSLQPLSFQNPVYHLNNPPAPLPKASVDSSLENLSTASSQSRSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTVPDKHVPIALPRQNSSSQAQIRKMDQAGLGARAKAPQSLPHSASLRSTGSMSAVSGAVVTEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEVEEDTEQNPDEAKHAEKYEQEIAKLKERLKVSSRRLEEYERRLLVQEQQMQKLLMEYKSRLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQVINGNEIIQTGEADRVPGLCQHAADECPDAGEGALQHASPQWHLPHQPHQAFHHGEW

Target ORF information:

RefSeq Version	XM_015290510.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015290510.2

ORF Insert Sequence:

ATGAGTGCTT TTAAAAAGTA TTACAGTCTC TCTCCCAGGA CCTTTAGGAG GTCTTTGTCG 
GAGCAAGATG CTGGCACCGC TGCCTTAAAG GACTATAATT GGTACCACCA AATGAGAAGC 
AAGAAGGGGA GCAAACAAAC AGCTTTGGAC CGAGAGAAAG CACTCGGAGC GTACAGGTGG 
CAAACACATC CCCGTCTTCC CTGGAGGACA AGCAGAAGAG CACACTCTTG CAGCCTCAGA 
GGGGAAGGTA ATGATTCGAG TCTTGTCAAA AGTTCTGCAA GCATGGATGC TACCAGCGCC 
GACGATCTCC ACCAAGACTC AACGGAGATG CTCTTCCAGG CAAGGACCAG ATCACCGCGG 
GTTCCCTTCA ACCGCTCCCT GTCTGAACCT GTGCCGCACT CTGCAAACAG GAATGCCAAG 
CCCTTTCTGC AGGCGGTGCG CTCTGTGGAT TGTGAGCAGC ACAGCTATTT TGTCCGTGGC 
ATCTTCTCAA CTCTCTCAAG TGGCTTTTCA AAGATAATAA ATCGAAGGGG TGAGCAGCCC 
CCCACTGAGC CACTGGAGGA GTGGAAACCA GCCGGTAGTC AAATTGACCC GTGCCCAGGT 
AATGGACTCT GTTCTGTTTG TGTTACAGAT GTAAAAGGAC CACCTACGCA CAGGCTGTCC 
TGCGGCCAGT CCCCCTACAC CGAGACGACA ACATGGGAGA GGAAGTACTG CATCCTGACT 
GACAGCCAGC TGGTGCTGCT CAACAGGGAG AAGGAGGTGC CCACCGAGGG GTTGCAGGAG 
CCGCAGGATG CAACCAAAGG CCGCTGTCTG CGCCGCACGG TGAGCGTGCC CTCCGAGGGC 
CAGTTCCCCG AGTACCCACC AGATGGGGCC GCCAAGCTGG AGGTCCCGGC AGAGAGATCT 
CCTCGCAGAC GGAGCATCTC TGGGACCAGC ACATCTGAGA AAACCAACTC CATGGATGCT 
GCAAATACCT CTCCTTTCAA AGTATCGGGC TTCTTCAGTA AACGTCTGAA AGGGTCCATC 
AAGAGGACAA AGAGTCAGTC AAAGCTGGAC AGGAACACAA GTTTCCGCCT CCCATCCCTC 
CGCAATGCTG ATGACAGGTC ACGTGGGCTG CCGAAGCTGA AGGAGTCCCG CTCTCATGAG 
TCCCTGCTGA GCCCCGGCAG CGCAGTGGAA GCCCTGGACC TTGGGGCAGA AGAAAAGGTC 
TTTGTAAAGC CACTGCACAG CAGCATCCTC GGACAGGACT TCTGCTTTGA GGTAACCTAC 
TCCAGCGGCA GTAAATGCTT CAGCTGTTCC TCTGCTGCAG AGAGGGATAA GTGGATGGAA 
AACCTACGCA GAACAGTGCA GCCGAATAAG GACAACTGCC GTCGAGCTGA AAATGTGCTC 
CGTCTCTGGA TCATTGAGGC AAAGGACCTG GCCCCCAAGA AGAAATACTT CTGTGAGCTG 
TGCCTTGATG ACACTCTCTT TGCCCGCACC ACCAGCAAAA CGAAAGCAGA TAACATTTTC 
TGGGGGGAGC ACTTTGAGTT CTACGGCCTC CCAGCTCTTC ACAGCATCAC GGTTCACATC 
TACAAGGATG TGGAGAAGAA AAAGAAAAAG GACAAAAACA ATTACGTCGG CCTGGTCAAC 
ATTCCCATGG CCACTGTAAC GGGGCGCCAG TTTGTGGAAA AATGGTACCC AGTCAGCACA 
CCTACACCCA ACAAAGGGAA ATCAGGAGGC CCTTCCATTC GCATCAAGTC GCGTTATCAG 
ACCATCACCA TCTTACCCAT GGAGCAATAC AAGGAGTTTG CAGAGTTTGT TACCAGCAAC 
TACACGATGC TGTGCTCTGT GCTGGAGCCA GTCATCAGCG TAAGGAATAA GGAAGAGATG 
GCTTGTGCAA TGGTGCACAT TCTTCAGAGC ACGGGGAGAG CCAAGGACTT CCTAACGGAT 
CTGGTGATGT CTGAGGTGGA TCGCTGCGGG GAACACGATG TCTTGATCTT CAGGGAGAAC 
ACACTTGCTA CCAAAGCTAT TGAGGAATAC CTGAAGTTGG TAGGGCAGAA GTATCTCCAT 
GATGCGCTAG GGGAGTTTAT CAAGGCTTTG TATGAGTCAG ATGAAAACTG TGAAGTGGAC 
CCCAGCAAAT GTTCATCCAG TGAATTAACA GACCATCAGA GCAACCTGAA AATGTGTTGT 
GAGCTGGCCT TCTGCAAGAT TATCAATTCC TACTGCGTGT TCCCTCGTGA GCTGAAGGAG 
GTGTTTGCAT CTTGGAAGCA GCAGTGCCTG AGCAGGGGCA AGCAGGACAT CAGCGAGCGG 
CTGATCAGTG CCTCCCTGTT CCTCCGCTTC CTGTGCCCCG CCATCATGTC CCCCAGCCTC 
TTCAGCCTCA TGCAGGAGTA TCCTGACGAC CGGACATCGC GCACGCTCAC ACTCATTGCT 
AAAGTCATTC AGAATCTCGC CAATTTTGCT AAGTTTGGTA ACAAGGAAGA GTACATGGCT 
TTCATGAACG ACTTTCTGGA ACATGAGTGG GGGGGAATGA AGCGATTTCT CCTGGAGATC 
TCTAATCCAG ATACCATCTC AAACATGCCT GGATTTGAGG GCTATATCGA CCTGGGCAGA 
GAGCTCTCAG TTTTGCATTC CCTCTTATGG GAGGTTGTGT CCCAACTTGA TAAGGGTGAA 
AATTCCTTCC TACAGGCGAC CGTGGCAAAA CTGGGACCTC TTCCTCGTAT TCTTGCCGAC 
ATCACCAAAT CAATGACCAA CCCTACGCCA ATACAGCAGC AGCTGAGGCG TTTCACTGAG 
CACAGCTCTA GTCCAAACGT CAGTGGCAGT CTCTCCTCAG GGCTTCAGAA GATATTCGAG 
GACCCCACTG ATGGTGACTT GCATAAACTG AAGTCTCCAA CCCAGGAAAA CATAGATGGC 
TATTTCAGGG GCAAGACCTT GCTGTTGGTT CAGCAGGCGT CCACCCAGAG CATGACTTAC 
TCAGATAAGG ACGAAAGAGA GAGCATCTTG CCCAACGGAC GTAGCATCTC CCTCATGGAC 
CTGCAGGATC CCCATACCGC TCACAGCGAC CACGCTTCCA TGATGCACGA CGTGCCTTTG 
CGCTTGGCCG GCAGCCAGCT CTCCATCACC CAGGTGGCCA ACATCAAGCA GCTGTGGGAG 
ACCCAGAGCA CGCCCCAAAG CGCTCCCCAG GTGAGGAGGC CGCTGCACCC GGCTTTGAAC 
CAGCAGGGCA GCCTCCAGCC TCTGTCATTC CAGAACCCGG TGTACCACCT CAACAACCCA 
CCTGCGCCGT TGCCCAAGGC CTCCGTGGAC TCCAGCCTGG AGAACCTGAG CACCGCCAGC 
TCCCAGAGCC GAAGCAACAG CGAAGACTTC AAGCTCAGCG GGCCCAGCAA CAGCAGCATG 
GAGGACTTCA CCAAGCGCAG CACGCAGAGC GAGGACTTCT CTAGGCGGCA CACGGTCCCG 
GACAAGCACG TCCCCATCGC GTTGCCCCGC CAGAACAGCA GCAGCCAGGC ACAGATCCGC 
AAAATGGACC AGGCCGGGTT AGGGGCCAGG GCCAAAGCGC CGCAGTCGCT GCCGCACAGC 
GCGTCCCTGC GCAGCACGGG CAGCATGTCG GCCGTGTCGG GGGCCGTGGT GACTGAGCCC 
GTCCAGAACG GCAGCCGCTC CCGGCAGCAG TCGTCCTCCT CCAGGGAGAG CCCTGTGCCG 
AAGGTGAGGG CCATTCAGAG GCAGCAAACG CAGCAGGTCC AGTCTCCTGT GGACTCAGCC 
ACAATGTCAC CAGTGGAAAG GACAGCAGCG TGGGTTCTCA ATAACGGGCA GTACGAGGAG 
GTCGAGGAGG ACACAGAGCA GAACCCAGAT GAAGCCAAGC ATGCTGAGAA GTACGAACAA 
GAGATAGCAA AGCTGAAGGA ACGCTTGAAA GTGTCGAGCC GCCGGCTGGA GGAGTACGAG 
CGGCGGCTCC TGGTGCAGGA GCAGCAAATG CAGAAGCTGC TGATGGAGTA CAAGTCCAGG 
CTGGAGGACA GCGAGGAGAG ACTGCGCAGG CAACAGGAGG AGAAGGACAG CCAGATGAAA 
AGCATTATCA GCAGGCTCAT GGCAGTTGAA GAGGAACTGA AGAAAGATCA TGCAGAGATG 
CAAGCTGTCA TCGATGCAAA GCAGAAAATT ATTGATGCAC AGGTCATTAA TGGGAATGAG 
ATAATTCAAA CAGGAGAAGC GGATCGTGTC CCTGGACTCT GCCAACACGC GGCTGATGAG 
TGCCCTGACG CAGGTGAAGG AGCGCTACAG CATGCAAGTC CGCAATGGCA TCTCCCCCAC 
CAACCCCACC AAGCTTTCCA TCACGGAGAA TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_004943245.3 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004943302.2 Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OGa152362
Clone ID Related Accession (Same CDS sequence)	XM_004943245.3
Accession Version	XM_004943245.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4287bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	ras GTPase-activating protein nGAP isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006095.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004943245.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGCTT TTAAAAAGTA TTACAGTCTC TCTCCCAGGA CCTTTAGGAG GTCTTTGTCG 
GAGCAAGATG CTGGCACCGC TGCCTTAAAG GACTATAATT GGTACCACCA AATGAGAAGC 
AAGAAGGGGA GCAAACAAAC AGCTTTGGAC CGAGAGAAAG CACTCGGAGC GTACAGGTGG 
CAAACACATC CCCGTCTTCC CTGGAGGACA AGCAGAAGAG CACACTCTTG CAGCCTCAGA 
GGGGAAGGTA ATGATTCGAG TCTTGTCAAA AGTTCTGCAA GCATGGATGC TACCAGCGCC 
GACGATCTCC ACCAAGACTC AACGGAGATG CTCTTCCAGG CAAGGACCAG ATCACCGCGG 
GTTCCCTTCA ACCGCTCCCT GTCTGAACCT GTGCCGCACT CTGCAAACAG GAATGCCAAG 
CCCTTTCTGC AGGCGGTGCG CTCTGTGGAT TGTGAGCAGC ACAGCTATTT TGTCCGTGGC 
ATCTTCTCAA CTCTCTCAAG TGGCTTTTCA AAGATAATAA ATCGAAGGGG TGAGCAGCCC 
CCCACTGAGC CACTGGAGGA GTGGAAACCA GCCGGTAGTC AAATTGACCC GTGCCCAGGT 
AATGGACTCT GTTCTGTTTG TGTTACAGAT GTAAAAGGAC CACCTACGCA CAGGCTGTCC 
TGCGGCCAGT CCCCCTACAC CGAGACGACA ACATGGGAGA GGAAGTACTG CATCCTGACT 
GACAGCCAGC TGGTGCTGCT CAACAGGGAG AAGGAGGTGC CCACCGAGGG GTTGCAGGAG 
CCGCAGGATG CAACCAAAGG CCGCTGTCTG CGCCGCACGG TGAGCGTGCC CTCCGAGGGC 
CAGTTCCCCG AGTACCCACC AGATGGGGCC GCCAAGCTGG AGGTCCCGGC AGAGAGATCT 
CCTCGCAGAC GGAGCATCTC TGGGACCAGC ACATCTGAGA AAACCAACTC CATGGATGCT 
GCAAATACCT CTCCTTTCAA AGTATCGGGC TTCTTCAGTA AACGTCTGAA AGGGTCCATC 
AAGAGGACAA AGAGTCAGTC AAAGCTGGAC AGGAACACAA GTTTCCGCCT CCCATCCCTC 
CGCAATGCTG ATGACAGGTC ACGTGGGCTG CCGAAGCTGA AGGAGTCCCG CTCTCATGAG 
TCCCTGCTGA GCCCCGGCAG CGCAGTGGAA GCCCTGGACC TTGGGGCAGA AGAAAAGGTC 
TTTGTAAAGC CACTGCACAG CAGCATCCTC GGACAGGACT TCTGCTTTGA GGTAACCTAC 
TCCAGCGGCA GTAAATGCTT CAGCTGTTCC TCTGCTGCAG AGAGGGATAA GTGGATGGAA 
AACCTACGCA GAACAGTGCA GCCGAATAAG GACAACTGCC GTCGAGCTGA AAATGTGCTC 
CGTCTCTGGA TCATTGAGGC AAAGGACCTG GCCCCCAAGA AGAAATACTT CTGTGAGCTG 
TGCCTTGATG ACACTCTCTT TGCCCGCACC ACCAGCAAAA CGAAAGCAGA TAACATTTTC 
TGGGGGGAGC ACTTTGAGTT CTACGGCCTC CCAGCTCTTC ACAGCATCAC GGTTCACATC 
TACAAGGATG TGGAGAAGAA AAAGAAAAAG GACAAAAACA ATTACGTCGG CCTGGTCAAC 
ATTCCCATGG CCACTGTAAC GGGGCGCCAG TTTGTGGAAA AATGGTACCC AGTCAGCACA 
CCTACACCCA ACAAAGGGAA ATCAGGAGGC CCTTCCATTC GCATCAAGTC GCGTTATCAG 
ACCATCACCA TCTTACCCAT GGAGCAATAC AAGGAGTTTG CAGAGTTTGT TACCAGCAAC 
TACACGATGC TGTGCTCTGT GCTGGAGCCA GTCATCAGCG TAAGGAATAA GGAAGAGATG 
GCTTGTGCAA TGGTGCACAT TCTTCAGAGC ACGGGGAGAG CCAAGGACTT CCTAACGGAT 
CTGGTGATGT CTGAGGTGGA TCGCTGCGGG GAACACGATG TCTTGATCTT CAGGGAGAAC 
ACACTTGCTA CCAAAGCTAT TGAGGAATAC CTGAAGTTGG TAGGGCAGAA GTATCTCCAT 
GATGCGCTAG GGGAGTTTAT CAAGGCTTTG TATGAGTCAG ATGAAAACTG TGAAGTGGAC 
CCCAGCAAAT GTTCATCCAG TGAATTAACA GACCATCAGA GCAACCTGAA AATGTGTTGT 
GAGCTGGCCT TCTGCAAGAT TATCAATTCC TACTGCGTGT TCCCTCGTGA GCTGAAGGAG 
GTGTTTGCAT CTTGGAAGCA GCAGTGCCTG AGCAGGGGCA AGCAGGACAT CAGCGAGCGG 
CTGATCAGTG CCTCCCTGTT CCTCCGCTTC CTGTGCCCCG CCATCATGTC CCCCAGCCTC 
TTCAGCCTCA TGCAGGAGTA TCCTGACGAC CGGACATCGC GCACGCTCAC ACTCATTGCT 
AAAGTCATTC AGAATCTCGC CAATTTTGCT AAGTTTGGTA ACAAGGAAGA GTACATGGCT 
TTCATGAACG ACTTTCTGGA ACATGAGTGG GGGGGAATGA AGCGATTTCT CCTGGAGATC 
TCTAATCCAG ATACCATCTC AAACATGCCT GGATTTGAGG GCTATATCGA CCTGGGCAGA 
GAGCTCTCAG TTTTGCATTC CCTCTTATGG GAGGTTGTGT CCCAACTTGA TAAGGGTGAA 
AATTCCTTCC TACAGGCGAC CGTGGCAAAA CTGGGACCTC TTCCTCGTAT TCTTGCCGAC 
ATCACCAAAT CAATGACCAA CCCTACGCCA ATACAGCAGC AGCTGAGGCG TTTCACTGAG 
CACAGCTCTA GTCCAAACGT CAGTGGCAGT CTCTCCTCAG GGCTTCAGAA GATATTCGAG 
GACCCCACTG ATGGTGACTT GCATAAACTG AAGTCTCCAA CCCAGGAAAA CATAGATGGC 
TATTTCAGGG GCAAGACCTT GCTGTTGGTT CAGCAGGCGT CCACCCAGAG CATGACTTAC 
TCAGATAAGG ACGAAAGAGA GAGCATCTTG CCCAACGGAC GTAGCATCTC CCTCATGGAC 
CTGCAGGATC CCCATACCGC TCACAGCGAC CACGCTTCCA TGATGCACGA CGTGCCTTTG 
CGCTTGGCCG GCAGCCAGCT CTCCATCACC CAGGTGGCCA ACATCAAGCA GCTGTGGGAG 
ACCCAGAGCA CGCCCCAAAG CGCTCCCCAG GTGAGGAGGC CGCTGCACCC GGCTTTGAAC 
CAGCAGGGCA GCCTCCAGCC TCTGTCATTC CAGAACCCGG TGTACCACCT CAACAACCCA 
CCTGCGCCGT TGCCCAAGGC CTCCGTGGAC TCCAGCCTGG AGAACCTGAG CACCGCCAGC 
TCCCAGAGCC GAAGCAACAG CGAAGACTTC AAGCTCAGCG GGCCCAGCAA CAGCAGCATG 
GAGGACTTCA CCAAGCGCAG CACGCAGAGC GAGGACTTCT CTAGGCGGCA CACGGTCCCG 
GACAAGCACG TCCCCATCGC GTTGCCCCGC CAGAACAGCA GCAGCCAGGC ACAGATCCGC 
AAAATGGACC AGGCCGGGTT AGGGGCCAGG GCCAAAGCGC CGCAGTCGCT GCCGCACAGC 
GCGTCCCTGC GCAGCACGGG CAGCATGTCG GCCGTGTCGG GGGCCGTGGT GACTGAGCCC 
GTCCAGAACG GCAGCCGCTC CCGGCAGCAG TCGTCCTCCT CCAGGGAGAG CCCTGTGCCG 
AAGGTGAGGG CCATTCAGAG GCAGCAAACG CAGCAGGTCC AGTCTCCTGT GGACTCAGCC 
ACAATGTCAC CAGTGGAAAG GACAGCAGCG TGGGTTCTCA ATAACGGGCA GTACGAGGAG 
GTCGAGGAGG ACACAGAGCA GAACCCAGAT GAAGCCAAGC ATGCTGAGAA GTACGAACAA 
GAGATAGCAA AGCTGAAGGA ACGCTTGAAA GTGTCGAGCC GCCGGCTGGA GGAGTACGAG 
CGGCGGCTCC TGGTGCAGGA GCAGCAAATG CAGAAGCTGC TGATGGAGTA CAAGTCCAGG 
CTGGAGGACA GCGAGGAGAG ACTGCGCAGG CAACAGGAGG AGAAGGACAG CCAGATGAAA 
AGCATTATCA GCAGGCTCAT GGCAGTTGAA GAGGAACTGA AGAAAGATCA TGCAGAGATG 
CAAGCTGTCA TCGATGCAAA GCAGAAAATT ATTGATGCAC AGGAGAAGCG GATCGTGTCC 
CTGGACTCTG CCAACACGCG GCTGATGAGT GCCCTGACGC AGGTGAAGGA GCGCTACAGC 
ATGCAAGTCC GCAATGGCAT CTCCCCCACC AACCCCACCA AGCTTTCCAT CACGGAGAAT 
GGTGAATTCA AAAACAGCAG CTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004943302.2
CDS	632..4918
Translation	MSAFKKYYSLSPRTFRRSLSEQDAGTAALKDYNWYHQMRSKKGSKQTALDREKALGAYRWQTHPRLPWRTSRRAHSCSLRGEGNDSSLVKSSASMDATSADDLHQDSTEMLFQARTRSPRVPFNRSLSEPVPHSANRNAKPFLQAVRSVDCEQHSYFVRGIFSTLSSGFSKIINRRGEQPPTEPLEEWKPAGSQIDPCPGNGLCSVCVTDVKGPPTHRLSCGQSPYTETTTWERKYCILTDSQLVLLNREKEVPTEGLQEPQDATKGRCLRRTVSVPSEGQFPEYPPDGAAKLEVPAERSPRRRSISGTSTSEKTNSMDAANTSPFKVSGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNADDRSRGLPKLKESRSHESLLSPGSAVEALDLGAEEKVFVKPLHSSILGQDFCFEVTYSSGSKCFSCSSAAERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYGLPALHSITVHIYKDVEKKKKKDKNNYVGLVNIPMATVTGRQFVEKWYPVSTPTPNKGKSGGPSIRIKSRYQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEEMACAMVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTLATKAIEEYLKLVGQKYLHDALGEFIKALYESDENCEVDPSKCSSSELTDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCLSRGKQDISERLISASLFLRFLCPAIMSPSLFSLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNMPGFEGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVAKLGPLPRILADITKSMTNPTPIQQQLRRFTEHSSSPNVSGSLSSGLQKIFEDPTDGDLHKLKSPTQENIDGYFRGKTLLLVQQASTQSMTYSDKDERESILPNGRSISLMDLQDPHTAHSDHASMMHDVPLRLAGSQLSITQVANIKQLWETQSTPQSAPQVRRPLHPALNQQGSLQPLSFQNPVYHLNNPPAPLPKASVDSSLENLSTASSQSRSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTVPDKHVPIALPRQNSSSQAQIRKMDQAGLGARAKAPQSLPHSASLRSTGSMSAVSGAVVTEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEVEEDTEQNPDEAKHAEKYEQEIAKLKERLKVSSRRLEEYERRLLVQEQQMQKLLMEYKSRLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQEKRIVSLDSANTRLMSALTQVKERYSMQVRNGISPTNPTKLSITENGEFKNSSC

Target ORF information:

RefSeq Version	XM_004943245.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004943245.3

ORF Insert Sequence:

ATGAGTGCTT TTAAAAAGTA TTACAGTCTC TCTCCCAGGA CCTTTAGGAG GTCTTTGTCG 
GAGCAAGATG CTGGCACCGC TGCCTTAAAG GACTATAATT GGTACCACCA AATGAGAAGC 
AAGAAGGGGA GCAAACAAAC AGCTTTGGAC CGAGAGAAAG CACTCGGAGC GTACAGGTGG 
CAAACACATC CCCGTCTTCC CTGGAGGACA AGCAGAAGAG CACACTCTTG CAGCCTCAGA 
GGGGAAGGTA ATGATTCGAG TCTTGTCAAA AGTTCTGCAA GCATGGATGC TACCAGCGCC 
GACGATCTCC ACCAAGACTC AACGGAGATG CTCTTCCAGG CAAGGACCAG ATCACCGCGG 
GTTCCCTTCA ACCGCTCCCT GTCTGAACCT GTGCCGCACT CTGCAAACAG GAATGCCAAG 
CCCTTTCTGC AGGCGGTGCG CTCTGTGGAT TGTGAGCAGC ACAGCTATTT TGTCCGTGGC 
ATCTTCTCAA CTCTCTCAAG TGGCTTTTCA AAGATAATAA ATCGAAGGGG TGAGCAGCCC 
CCCACTGAGC CACTGGAGGA GTGGAAACCA GCCGGTAGTC AAATTGACCC GTGCCCAGGT 
AATGGACTCT GTTCTGTTTG TGTTACAGAT GTAAAAGGAC CACCTACGCA CAGGCTGTCC 
TGCGGCCAGT CCCCCTACAC CGAGACGACA ACATGGGAGA GGAAGTACTG CATCCTGACT 
GACAGCCAGC TGGTGCTGCT CAACAGGGAG AAGGAGGTGC CCACCGAGGG GTTGCAGGAG 
CCGCAGGATG CAACCAAAGG CCGCTGTCTG CGCCGCACGG TGAGCGTGCC CTCCGAGGGC 
CAGTTCCCCG AGTACCCACC AGATGGGGCC GCCAAGCTGG AGGTCCCGGC AGAGAGATCT 
CCTCGCAGAC GGAGCATCTC TGGGACCAGC ACATCTGAGA AAACCAACTC CATGGATGCT 
GCAAATACCT CTCCTTTCAA AGTATCGGGC TTCTTCAGTA AACGTCTGAA AGGGTCCATC 
AAGAGGACAA AGAGTCAGTC AAAGCTGGAC AGGAACACAA GTTTCCGCCT CCCATCCCTC 
CGCAATGCTG ATGACAGGTC ACGTGGGCTG CCGAAGCTGA AGGAGTCCCG CTCTCATGAG 
TCCCTGCTGA GCCCCGGCAG CGCAGTGGAA GCCCTGGACC TTGGGGCAGA AGAAAAGGTC 
TTTGTAAAGC CACTGCACAG CAGCATCCTC GGACAGGACT TCTGCTTTGA GGTAACCTAC 
TCCAGCGGCA GTAAATGCTT CAGCTGTTCC TCTGCTGCAG AGAGGGATAA GTGGATGGAA 
AACCTACGCA GAACAGTGCA GCCGAATAAG GACAACTGCC GTCGAGCTGA AAATGTGCTC 
CGTCTCTGGA TCATTGAGGC AAAGGACCTG GCCCCCAAGA AGAAATACTT CTGTGAGCTG 
TGCCTTGATG ACACTCTCTT TGCCCGCACC ACCAGCAAAA CGAAAGCAGA TAACATTTTC 
TGGGGGGAGC ACTTTGAGTT CTACGGCCTC CCAGCTCTTC ACAGCATCAC GGTTCACATC 
TACAAGGATG TGGAGAAGAA AAAGAAAAAG GACAAAAACA ATTACGTCGG CCTGGTCAAC 
ATTCCCATGG CCACTGTAAC GGGGCGCCAG TTTGTGGAAA AATGGTACCC AGTCAGCACA 
CCTACACCCA ACAAAGGGAA ATCAGGAGGC CCTTCCATTC GCATCAAGTC GCGTTATCAG 
ACCATCACCA TCTTACCCAT GGAGCAATAC AAGGAGTTTG CAGAGTTTGT TACCAGCAAC 
TACACGATGC TGTGCTCTGT GCTGGAGCCA GTCATCAGCG TAAGGAATAA GGAAGAGATG 
GCTTGTGCAA TGGTGCACAT TCTTCAGAGC ACGGGGAGAG CCAAGGACTT CCTAACGGAT 
CTGGTGATGT CTGAGGTGGA TCGCTGCGGG GAACACGATG TCTTGATCTT CAGGGAGAAC 
ACACTTGCTA CCAAAGCTAT TGAGGAATAC CTGAAGTTGG TAGGGCAGAA GTATCTCCAT 
GATGCGCTAG GGGAGTTTAT CAAGGCTTTG TATGAGTCAG ATGAAAACTG TGAAGTGGAC 
CCCAGCAAAT GTTCATCCAG TGAATTAACA GACCATCAGA GCAACCTGAA AATGTGTTGT 
GAGCTGGCCT TCTGCAAGAT TATCAATTCC TACTGCGTGT TCCCTCGTGA GCTGAAGGAG 
GTGTTTGCAT CTTGGAAGCA GCAGTGCCTG AGCAGGGGCA AGCAGGACAT CAGCGAGCGG 
CTGATCAGTG CCTCCCTGTT CCTCCGCTTC CTGTGCCCCG CCATCATGTC CCCCAGCCTC 
TTCAGCCTCA TGCAGGAGTA TCCTGACGAC CGGACATCGC GCACGCTCAC ACTCATTGCT 
AAAGTCATTC AGAATCTCGC CAATTTTGCT AAGTTTGGTA ACAAGGAAGA GTACATGGCT 
TTCATGAACG ACTTTCTGGA ACATGAGTGG GGGGGAATGA AGCGATTTCT CCTGGAGATC 
TCTAATCCAG ATACCATCTC AAACATGCCT GGATTTGAGG GCTATATCGA CCTGGGCAGA 
GAGCTCTCAG TTTTGCATTC CCTCTTATGG GAGGTTGTGT CCCAACTTGA TAAGGGTGAA 
AATTCCTTCC TACAGGCGAC CGTGGCAAAA CTGGGACCTC TTCCTCGTAT TCTTGCCGAC 
ATCACCAAAT CAATGACCAA CCCTACGCCA ATACAGCAGC AGCTGAGGCG TTTCACTGAG 
CACAGCTCTA GTCCAAACGT CAGTGGCAGT CTCTCCTCAG GGCTTCAGAA GATATTCGAG 
GACCCCACTG ATGGTGACTT GCATAAACTG AAGTCTCCAA CCCAGGAAAA CATAGATGGC 
TATTTCAGGG GCAAGACCTT GCTGTTGGTT CAGCAGGCGT CCACCCAGAG CATGACTTAC 
TCAGATAAGG ACGAAAGAGA GAGCATCTTG CCCAACGGAC GTAGCATCTC CCTCATGGAC 
CTGCAGGATC CCCATACCGC TCACAGCGAC CACGCTTCCA TGATGCACGA CGTGCCTTTG 
CGCTTGGCCG GCAGCCAGCT CTCCATCACC CAGGTGGCCA ACATCAAGCA GCTGTGGGAG 
ACCCAGAGCA CGCCCCAAAG CGCTCCCCAG GTGAGGAGGC CGCTGCACCC GGCTTTGAAC 
CAGCAGGGCA GCCTCCAGCC TCTGTCATTC CAGAACCCGG TGTACCACCT CAACAACCCA 
CCTGCGCCGT TGCCCAAGGC CTCCGTGGAC TCCAGCCTGG AGAACCTGAG CACCGCCAGC 
TCCCAGAGCC GAAGCAACAG CGAAGACTTC AAGCTCAGCG GGCCCAGCAA CAGCAGCATG 
GAGGACTTCA CCAAGCGCAG CACGCAGAGC GAGGACTTCT CTAGGCGGCA CACGGTCCCG 
GACAAGCACG TCCCCATCGC GTTGCCCCGC CAGAACAGCA GCAGCCAGGC ACAGATCCGC 
AAAATGGACC AGGCCGGGTT AGGGGCCAGG GCCAAAGCGC CGCAGTCGCT GCCGCACAGC 
GCGTCCCTGC GCAGCACGGG CAGCATGTCG GCCGTGTCGG GGGCCGTGGT GACTGAGCCC 
GTCCAGAACG GCAGCCGCTC CCGGCAGCAG TCGTCCTCCT CCAGGGAGAG CCCTGTGCCG 
AAGGTGAGGG CCATTCAGAG GCAGCAAACG CAGCAGGTCC AGTCTCCTGT GGACTCAGCC 
ACAATGTCAC CAGTGGAAAG GACAGCAGCG TGGGTTCTCA ATAACGGGCA GTACGAGGAG 
GTCGAGGAGG ACACAGAGCA GAACCCAGAT GAAGCCAAGC ATGCTGAGAA GTACGAACAA 
GAGATAGCAA AGCTGAAGGA ACGCTTGAAA GTGTCGAGCC GCCGGCTGGA GGAGTACGAG 
CGGCGGCTCC TGGTGCAGGA GCAGCAAATG CAGAAGCTGC TGATGGAGTA CAAGTCCAGG 
CTGGAGGACA GCGAGGAGAG ACTGCGCAGG CAACAGGAGG AGAAGGACAG CCAGATGAAA 
AGCATTATCA GCAGGCTCAT GGCAGTTGAA GAGGAACTGA AGAAAGATCA TGCAGAGATG 
CAAGCTGTCA TCGATGCAAA GCAGAAAATT ATTGATGCAC AGGAGAAGCG GATCGTGTCC 
CTGGACTCTG CCAACACGCG GCTGATGAGT GCCCTGACGC AGGTGAAGGA GCGCTACAGC 
ATGCAAGTCC GCAATGGCAT CTCCCCCACC AACCCCACCA AGCTTTCCAT CACGGAGAAT 
GGTGAATTCA AAAACAGCAG CTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_015290511.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015145997.1 Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OGa152363
Clone ID Related Accession (Same CDS sequence)	XM_015290511.2
Accession Version	XM_015290511.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4275bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	ras GTPase-activating protein nGAP isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006095.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015290511.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGCTT TTAAAAAGTA TTACAGTCTC TCTCCCAGGA CCTTTAGGAG GTCTTTGTCG 
GAGCAAGATG CTGGCACCGC TGCCTTAAAG GACTATAATT GGTACCACCA AATGAGAAGC 
AAGAAGGGGA GCAAACAAAC AGCTTTGGAC CGAGAGAAAG CACTCGGAGC GTACAGGTGG 
CAAACACATC CCCGTCTTCC CTGGAGGACA AGCAGAAGAG CACACTCTTG CAGCCTCAGA 
GGGGAAGGTA ATGATTCGAG TCTTGTCAAA AGTTCTGCAA GCATGGATGC TACCAGCGCC 
GACGATCTCC ACCAAGACTC AACGGAGATG CTCTTCCAGG CAAGGACCAG ATCACCGCGG 
GTTCCCTTCA ACCGCTCCCT GTCTGAACCT GTGCCGCACT CTGCAAACAG GAATGCCAAG 
CCCTTTCTGC AGGCGGTGCG CTCTGTGGAT TGTGAGCAGC ACAGCTATTT TGTCCGTGGC 
ATCTTCTCAA CTCTCTCAAG TGGCTTTTCA AAGATAATAA ATCGAAGGGG TGAGCAGCCC 
CCCACTGAGC CACTGGAGGA GTGGAAACCA GCCGGTAGTC AAATTGACCC GTGCCCAGGT 
AATGGACTCT GTTCTGTTTG TGTTACAGAT GTAAAAGGAC CACCTACGCA CAGGCTGTCC 
TGCGGCCAGT CCCCCTACAC CGAGACGACA ACATGGGAGA GGAAGTACTG CATCCTGACT 
GACAGCCAGC TGGTGCTGCT CAACAGGGAG AAGGAGGTGC CCACCGAGGG GTTGCAGGAG 
CCGCAGGATG CAACCAAAGG CCGCTGTCTG CGCCGCACGG TGAGCGTGCC CTCCGAGGGC 
CAGTTCCCCG AGTACCCACC AGATGGGGCC GCCAAGCTGG AGGTCCCGGC AGAGAGATCT 
CCTCGCAGAC GGAGCATCTC TGGGACCAGC ACATCTGAGA AAACCAACTC CATGGATGCT 
GCAAATACCT CTCCTTTCAA AGTATCGGGC TTCTTCAGTA AACGTCTGAA AGGGTCCATC 
AAGAGGACAA AGAGTCAGTC AAAGCTGGAC AGGAACACAA GTTTCCGCCT CCCATCCCTC 
CGCAATGCTG ATGACAGGTC ACGTGGGCTG CCGAAGCTGA AGGAGTCCCG CTCTCATGAG 
TCCCTGCTGA GCCCCGGCAG CGCAGTGGAA GCCCTGGACC TTGGGGCAGA AGAAAAGGTC 
TTTGTAAAGC CACTGCACAG CAGCATCCTC GGACAGGACT TCTGCTTTGA GGTAACCTAC 
TCCAGCGGCA GTAAATGCTT CAGCTGTTCC TCTGCTGCAG AGAGGGATAA GTGGATGGAA 
AACCTACGCA GAACAGTGCA GCCGAATAAG GACAACTGCC GTCGAGCTGA AAATGTGCTC 
CGTCTCTGGA TCATTGAGGC AAAGGACCTG GCCCCCAAGA AGAAATACTT CTGTGAGCTG 
TGCCTTGATG ACACTCTCTT TGCCCGCACC ACCAGCAAAA CGAAAGCAGA TAACATTTTC 
TGGGGGGAGC ACTTTGAGTT CTACGGCCTC CCAGCTCTTC ACAGCATCAC GGTTCACATC 
TACAAGGATG TGGAGAAGAA AAAGAAAAAG GACAAAAACA ATTACGTCGG CCTGGTCAAC 
ATTCCCATGG CCACTGTAAC GGGGCGCCAG TTTGTGGAAA AATGGTACCC AGTCAGCACA 
CCTACACCCA ACAAAGGGAA ATCAGGAGGC CCTTCCATTC GCATCAAGTC GCGTTATCAG 
ACCATCACCA TCTTACCCAT GGAGCAATAC AAGGAGTTTG CAGAGTTTGT TACCAGCAAC 
TACACGATGC TGTGCTCTGT GCTGGAGCCA GTCATCAGCG TAAGGAATAA GGAAGAGATG 
GCTTGTGCAA TGGTGCACAT TCTTCAGAGC ACGGGGAGAG CCAAGGACTT CCTAACGGAT 
CTGGTGATGT CTGAGGTGGA TCGCTGCGGG GAACACGATG TCTTGATCTT CAGGGAGAAC 
ACACTTGCTA CCAAAGCTAT TGAGGAATAC CTGAAGTTGG TAGGGCAGAA GTATCTCCAT 
GATGCGCTAG GGGAGTTTAT CAAGGCTTTG TATGAGTCAG ATGAAAACTG TGAAGTGGAC 
CCCAGCAAAT GTTCATCCAG TGAATTAACA GACCATCAGA GCAACCTGAA AATGTGTTGT 
GAGCTGGCCT TCTGCAAGAT TATCAATTCC TACTGCGTGT TCCCTCGTGA GCTGAAGGAG 
GTGTTTGCAT CTTGGAAGCA GCAGTGCCTG AGCAGGGGCA AGCAGGACAT CAGCGAGCGG 
CTGATCAGTG CCTCCCTGTT CCTCCGCTTC CTGTGCCCCG CCATCATGTC CCCCAGCCTC 
TTCAGCCTCA TGCAGGAGTA TCCTGACGAC CGGACATCGC GCACGCTCAC ACTCATTGCT 
AAAGTCATTC AGAATCTCGC CAATTTTGCT AAGTTTGGTA ACAAGGAAGA GTACATGGCT 
TTCATGAACG ACTTTCTGGA ACATGAGTGG GGGGGAATGA AGCGATTTCT CCTGGAGATC 
TCTAATCCAG ATACCATCTC AAACATGCCT GGATTTGAGG GCTATATCGA CCTGGGCAGA 
GAGCTCTCAG TTTTGCATTC CCTCTTATGG GAGGTTGTGT CCCAACTTGA TAAGGCGACC 
GTGGCAAAAC TGGGACCTCT TCCTCGTATT CTTGCCGACA TCACCAAATC AATGACCAAC 
CCTACGCCAA TACAGCAGCA GCTGAGGCGT TTCACTGAGC ACAGCTCTAG TCCAAACGTC 
AGTGGCAGTC TCTCCTCAGG GCTTCAGAAG ATATTCGAGG ACCCCACTGA TGGTGACTTG 
CATAAACTGA AGTCTCCAAC CCAGGAAAAC ATAGATGGCT ATTTCAGGGG CAAGACCTTG 
CTGTTGGTTC AGCAGGCGTC CACCCAGAGC ATGACTTACT CAGATAAGGA CGAAAGAGAG 
AGCATCTTGC CCAACGGACG TAGCATCTCC CTCATGGACC TGCAGGATCC CCATACCGCT 
CACAGCGACC ACGCTTCCAT GATGCACGAC GTGCCTTTGC GCTTGGCCGG CAGCCAGCTC 
TCCATCACCC AGGTGGCCAA CATCAAGCAG CTGTGGGAGA CCCAGAGCAC GCCCCAAAGC 
GCTCCCCAGG TGAGGAGGCC GCTGCACCCG GCTTTGAACC AGCAGGGCAG CCTCCAGCCT 
CTGTCATTCC AGAACCCGGT GTACCACCTC AACAACCCAC CTGCGCCGTT GCCCAAGGCC 
TCCGTGGACT CCAGCCTGGA GAACCTGAGC ACCGCCAGCT CCCAGAGCCG AAGCAACAGC 
GAAGACTTCA AGCTCAGCGG GCCCAGCAAC AGCAGCATGG AGGACTTCAC CAAGCGCAGC 
ACGCAGAGCG AGGACTTCTC TAGGCGGCAC ACGGTCCCGG ACAAGCACGT CCCCATCGCG 
TTGCCCCGCC AGAACAGCAG CAGCCAGGCA CAGATCCGCA AAATGGACCA GGCCGGGTTA 
GGGGCCAGGG CCAAAGCGCC GCAGTCGCTG CCGCACAGCG CGTCCCTGCG CAGCACGGGC 
AGCATGTCGG CCGTGTCGGG GGCCGTGGTG ACTGAGCCCG TCCAGAACGG CAGCCGCTCC 
CGGCAGCAGT CGTCCTCCTC CAGGGAGAGC CCTGTGCCGA AGGTGAGGGC CATTCAGAGG 
CAGCAAACGC AGCAGGTCCA GTCTCCTGTG GACTCAGCCA CAATGTCACC AGTGGAAAGG 
ACAGCAGCGT GGGTTCTCAA TAACGGGCAG TACGAGGAGG TCGAGGAGGA CACAGAGCAG 
AACCCAGATG AAGCCAAGCA TGCTGAGAAG TACGAACAAG AGATAGCAAA GCTGAAGGAA 
CGCTTGAAAG TGTCGAGCCG CCGGCTGGAG GAGTACGAGC GGCGGCTCCT GGTGCAGGAG 
CAGCAAATGC AGAAGCTGCT GATGGAGTAC AAGTCCAGGC TGGAGGACAG CGAGGAGAGA 
CTGCGCAGGC AACAGGAGGA GAAGGACAGC CAGATGAAAA GCATTATCAG CAGGCTCATG 
GCAGTTGAAG AGGAACTGAA GAAAGATCAT GCAGAGATGC AAGCTGTCAT CGATGCAAAG 
CAGAAAATTA TTGATGCACA GGTCATTAAT GGGAATGAGA TAATTCAAAC AGGAGAAGCG 
GATCGTGTCC CTGGACTCTG CCAACACGCG GCTGATGAGT GCCCTGACGC AGGTGAAGGA 
GCGCTACAGC ATGCAAGTCC GCAATGGCAT CTCCCCCACC AACCCCACCA AGCTTTCCAT 
CACGGAGAAT GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015145997.1
CDS	637..4911
Translation	MSAFKKYYSLSPRTFRRSLSEQDAGTAALKDYNWYHQMRSKKGSKQTALDREKALGAYRWQTHPRLPWRTSRRAHSCSLRGEGNDSSLVKSSASMDATSADDLHQDSTEMLFQARTRSPRVPFNRSLSEPVPHSANRNAKPFLQAVRSVDCEQHSYFVRGIFSTLSSGFSKIINRRGEQPPTEPLEEWKPAGSQIDPCPGNGLCSVCVTDVKGPPTHRLSCGQSPYTETTTWERKYCILTDSQLVLLNREKEVPTEGLQEPQDATKGRCLRRTVSVPSEGQFPEYPPDGAAKLEVPAERSPRRRSISGTSTSEKTNSMDAANTSPFKVSGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNADDRSRGLPKLKESRSHESLLSPGSAVEALDLGAEEKVFVKPLHSSILGQDFCFEVTYSSGSKCFSCSSAAERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYGLPALHSITVHIYKDVEKKKKKDKNNYVGLVNIPMATVTGRQFVEKWYPVSTPTPNKGKSGGPSIRIKSRYQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEEMACAMVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTLATKAIEEYLKLVGQKYLHDALGEFIKALYESDENCEVDPSKCSSSELTDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCLSRGKQDISERLISASLFLRFLCPAIMSPSLFSLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNMPGFEGYIDLGRELSVLHSLLWEVVSQLDKATVAKLGPLPRILADITKSMTNPTPIQQQLRRFTEHSSSPNVSGSLSSGLQKIFEDPTDGDLHKLKSPTQENIDGYFRGKTLLLVQQASTQSMTYSDKDERESILPNGRSISLMDLQDPHTAHSDHASMMHDVPLRLAGSQLSITQVANIKQLWETQSTPQSAPQVRRPLHPALNQQGSLQPLSFQNPVYHLNNPPAPLPKASVDSSLENLSTASSQSRSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTVPDKHVPIALPRQNSSSQAQIRKMDQAGLGARAKAPQSLPHSASLRSTGSMSAVSGAVVTEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEVEEDTEQNPDEAKHAEKYEQEIAKLKERLKVSSRRLEEYERRLLVQEQQMQKLLMEYKSRLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQVINGNEIIQTGEADRVPGLCQHAADECPDAGEGALQHASPQWHLPHQPHQAFHHGEW

Target ORF information:

RefSeq Version	XM_015290511.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015290511.2

ORF Insert Sequence:

ATGAGTGCTT TTAAAAAGTA TTACAGTCTC TCTCCCAGGA CCTTTAGGAG GTCTTTGTCG 
GAGCAAGATG CTGGCACCGC TGCCTTAAAG GACTATAATT GGTACCACCA AATGAGAAGC 
AAGAAGGGGA GCAAACAAAC AGCTTTGGAC CGAGAGAAAG CACTCGGAGC GTACAGGTGG 
CAAACACATC CCCGTCTTCC CTGGAGGACA AGCAGAAGAG CACACTCTTG CAGCCTCAGA 
GGGGAAGGTA ATGATTCGAG TCTTGTCAAA AGTTCTGCAA GCATGGATGC TACCAGCGCC 
GACGATCTCC ACCAAGACTC AACGGAGATG CTCTTCCAGG CAAGGACCAG ATCACCGCGG 
GTTCCCTTCA ACCGCTCCCT GTCTGAACCT GTGCCGCACT CTGCAAACAG GAATGCCAAG 
CCCTTTCTGC AGGCGGTGCG CTCTGTGGAT TGTGAGCAGC ACAGCTATTT TGTCCGTGGC 
ATCTTCTCAA CTCTCTCAAG TGGCTTTTCA AAGATAATAA ATCGAAGGGG TGAGCAGCCC 
CCCACTGAGC CACTGGAGGA GTGGAAACCA GCCGGTAGTC AAATTGACCC GTGCCCAGGT 
AATGGACTCT GTTCTGTTTG TGTTACAGAT GTAAAAGGAC CACCTACGCA CAGGCTGTCC 
TGCGGCCAGT CCCCCTACAC CGAGACGACA ACATGGGAGA GGAAGTACTG CATCCTGACT 
GACAGCCAGC TGGTGCTGCT CAACAGGGAG AAGGAGGTGC CCACCGAGGG GTTGCAGGAG 
CCGCAGGATG CAACCAAAGG CCGCTGTCTG CGCCGCACGG TGAGCGTGCC CTCCGAGGGC 
CAGTTCCCCG AGTACCCACC AGATGGGGCC GCCAAGCTGG AGGTCCCGGC AGAGAGATCT 
CCTCGCAGAC GGAGCATCTC TGGGACCAGC ACATCTGAGA AAACCAACTC CATGGATGCT 
GCAAATACCT CTCCTTTCAA AGTATCGGGC TTCTTCAGTA AACGTCTGAA AGGGTCCATC 
AAGAGGACAA AGAGTCAGTC AAAGCTGGAC AGGAACACAA GTTTCCGCCT CCCATCCCTC 
CGCAATGCTG ATGACAGGTC ACGTGGGCTG CCGAAGCTGA AGGAGTCCCG CTCTCATGAG 
TCCCTGCTGA GCCCCGGCAG CGCAGTGGAA GCCCTGGACC TTGGGGCAGA AGAAAAGGTC 
TTTGTAAAGC CACTGCACAG CAGCATCCTC GGACAGGACT TCTGCTTTGA GGTAACCTAC 
TCCAGCGGCA GTAAATGCTT CAGCTGTTCC TCTGCTGCAG AGAGGGATAA GTGGATGGAA 
AACCTACGCA GAACAGTGCA GCCGAATAAG GACAACTGCC GTCGAGCTGA AAATGTGCTC 
CGTCTCTGGA TCATTGAGGC AAAGGACCTG GCCCCCAAGA AGAAATACTT CTGTGAGCTG 
TGCCTTGATG ACACTCTCTT TGCCCGCACC ACCAGCAAAA CGAAAGCAGA TAACATTTTC 
TGGGGGGAGC ACTTTGAGTT CTACGGCCTC CCAGCTCTTC ACAGCATCAC GGTTCACATC 
TACAAGGATG TGGAGAAGAA AAAGAAAAAG GACAAAAACA ATTACGTCGG CCTGGTCAAC 
ATTCCCATGG CCACTGTAAC GGGGCGCCAG TTTGTGGAAA AATGGTACCC AGTCAGCACA 
CCTACACCCA ACAAAGGGAA ATCAGGAGGC CCTTCCATTC GCATCAAGTC GCGTTATCAG 
ACCATCACCA TCTTACCCAT GGAGCAATAC AAGGAGTTTG CAGAGTTTGT TACCAGCAAC 
TACACGATGC TGTGCTCTGT GCTGGAGCCA GTCATCAGCG TAAGGAATAA GGAAGAGATG 
GCTTGTGCAA TGGTGCACAT TCTTCAGAGC ACGGGGAGAG CCAAGGACTT CCTAACGGAT 
CTGGTGATGT CTGAGGTGGA TCGCTGCGGG GAACACGATG TCTTGATCTT CAGGGAGAAC 
ACACTTGCTA CCAAAGCTAT TGAGGAATAC CTGAAGTTGG TAGGGCAGAA GTATCTCCAT 
GATGCGCTAG GGGAGTTTAT CAAGGCTTTG TATGAGTCAG ATGAAAACTG TGAAGTGGAC 
CCCAGCAAAT GTTCATCCAG TGAATTAACA GACCATCAGA GCAACCTGAA AATGTGTTGT 
GAGCTGGCCT TCTGCAAGAT TATCAATTCC TACTGCGTGT TCCCTCGTGA GCTGAAGGAG 
GTGTTTGCAT CTTGGAAGCA GCAGTGCCTG AGCAGGGGCA AGCAGGACAT CAGCGAGCGG 
CTGATCAGTG CCTCCCTGTT CCTCCGCTTC CTGTGCCCCG CCATCATGTC CCCCAGCCTC 
TTCAGCCTCA TGCAGGAGTA TCCTGACGAC CGGACATCGC GCACGCTCAC ACTCATTGCT 
AAAGTCATTC AGAATCTCGC CAATTTTGCT AAGTTTGGTA ACAAGGAAGA GTACATGGCT 
TTCATGAACG ACTTTCTGGA ACATGAGTGG GGGGGAATGA AGCGATTTCT CCTGGAGATC 
TCTAATCCAG ATACCATCTC AAACATGCCT GGATTTGAGG GCTATATCGA CCTGGGCAGA 
GAGCTCTCAG TTTTGCATTC CCTCTTATGG GAGGTTGTGT CCCAACTTGA TAAGGCGACC 
GTGGCAAAAC TGGGACCTCT TCCTCGTATT CTTGCCGACA TCACCAAATC AATGACCAAC 
CCTACGCCAA TACAGCAGCA GCTGAGGCGT TTCACTGAGC ACAGCTCTAG TCCAAACGTC 
AGTGGCAGTC TCTCCTCAGG GCTTCAGAAG ATATTCGAGG ACCCCACTGA TGGTGACTTG 
CATAAACTGA AGTCTCCAAC CCAGGAAAAC ATAGATGGCT ATTTCAGGGG CAAGACCTTG 
CTGTTGGTTC AGCAGGCGTC CACCCAGAGC ATGACTTACT CAGATAAGGA CGAAAGAGAG 
AGCATCTTGC CCAACGGACG TAGCATCTCC CTCATGGACC TGCAGGATCC CCATACCGCT 
CACAGCGACC ACGCTTCCAT GATGCACGAC GTGCCTTTGC GCTTGGCCGG CAGCCAGCTC 
TCCATCACCC AGGTGGCCAA CATCAAGCAG CTGTGGGAGA CCCAGAGCAC GCCCCAAAGC 
GCTCCCCAGG TGAGGAGGCC GCTGCACCCG GCTTTGAACC AGCAGGGCAG CCTCCAGCCT 
CTGTCATTCC AGAACCCGGT GTACCACCTC AACAACCCAC CTGCGCCGTT GCCCAAGGCC 
TCCGTGGACT CCAGCCTGGA GAACCTGAGC ACCGCCAGCT CCCAGAGCCG AAGCAACAGC 
GAAGACTTCA AGCTCAGCGG GCCCAGCAAC AGCAGCATGG AGGACTTCAC CAAGCGCAGC 
ACGCAGAGCG AGGACTTCTC TAGGCGGCAC ACGGTCCCGG ACAAGCACGT CCCCATCGCG 
TTGCCCCGCC AGAACAGCAG CAGCCAGGCA CAGATCCGCA AAATGGACCA GGCCGGGTTA 
GGGGCCAGGG CCAAAGCGCC GCAGTCGCTG CCGCACAGCG CGTCCCTGCG CAGCACGGGC 
AGCATGTCGG CCGTGTCGGG GGCCGTGGTG ACTGAGCCCG TCCAGAACGG CAGCCGCTCC 
CGGCAGCAGT CGTCCTCCTC CAGGGAGAGC CCTGTGCCGA AGGTGAGGGC CATTCAGAGG 
CAGCAAACGC AGCAGGTCCA GTCTCCTGTG GACTCAGCCA CAATGTCACC AGTGGAAAGG 
ACAGCAGCGT GGGTTCTCAA TAACGGGCAG TACGAGGAGG TCGAGGAGGA CACAGAGCAG 
AACCCAGATG AAGCCAAGCA TGCTGAGAAG TACGAACAAG AGATAGCAAA GCTGAAGGAA 
CGCTTGAAAG TGTCGAGCCG CCGGCTGGAG GAGTACGAGC GGCGGCTCCT GGTGCAGGAG 
CAGCAAATGC AGAAGCTGCT GATGGAGTAC AAGTCCAGGC TGGAGGACAG CGAGGAGAGA 
CTGCGCAGGC AACAGGAGGA GAAGGACAGC CAGATGAAAA GCATTATCAG CAGGCTCATG 
GCAGTTGAAG AGGAACTGAA GAAAGATCAT GCAGAGATGC AAGCTGTCAT CGATGCAAAG 
CAGAAAATTA TTGATGCACA GGTCATTAAT GGGAATGAGA TAATTCAAAC AGGAGAAGCG 
GATCGTGTCC CTGGACTCTG CCAACACGCG GCTGATGAGT GCCCTGACGC AGGTGAAGGA 
GCGCTACAGC ATGCAAGTCC GCAATGGCAT CTCCCCCACC AACCCCACCA AGCTTTCCAT 
CACGGAGAAT GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_004943246.3 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004943303.2 Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X5, mRNA.

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CloneID	OGa152364
Clone ID Related Accession (Same CDS sequence)	XM_004943246.3
Accession Version	XM_004943246.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4266bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	ras GTPase-activating protein nGAP isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006095.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004943246.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGCTT TTAAAAAGTA TTACAGTCTC TCTCCCAGGA CCTTTAGGAG GTCTTTGTCG 
GAGCAAGATG CTGGCACCGC TGCCTTAAAG GACTATAATT GGTACCACCA AATGAGAAGC 
AAGAAGGGGA GCAAACAAAC AGCTTTGGAC CGAGAGAAAG CACTCGGAGC GTACAGGTGG 
CAAACACATC CCCGTCTTCC CTGGAGGACA AGCAGAAGAG CACACTCTTG CAGCCTCAGA 
GGGGAAGGTA ATGATTCGAG TCTTGTCAAA AGTTCTGCAA GCATGGATGC TACCAGCGCC 
GACGATCTCC ACCAAGACTC AACGGAGATG CTCTTCCAGG CAAGGACCAG ATCACCGCGG 
GTTCCCTTCA ACCGCTCCCT GTCTGAACCT GTGCCGCACT CTGCAAACAG GAATGCCAAG 
CCCTTTCTGC AGGCGGTGCG CTCTGTGGAT TGTGAGCAGC ACAGCTATTT TGTCCGTGGC 
ATCTTCTCAA CTCTCTCAAG TGGCTTTTCA AAGATAATAA ATCGAAGGGG TGAGCAGCCC 
CCCACTGAGC CACTGGAGGA GTGGAAACCA GCCGGTAGTC AAATTGACCC GTGCCCAGGT 
AATGGACTCT GTTCTGTTTG TGTTACAGAT GTAAAAGGAC CACCTACGCA CAGGCTGTCC 
TGCGGCCAGT CCCCCTACAC CGAGACGACA ACATGGGAGA GGAAGTACTG CATCCTGACT 
GACAGCCAGC TGGTGCTGCT CAACAGGGAG AAGGAGGTGC CCACCGAGGG GTTGCAGGAG 
CCGCAGGATG CAACCAAAGG CCGCTGTCTG CGCCGCACGG TGAGCGTGCC CTCCGAGGGC 
CAGTTCCCCG AGTACCCACC AGATGGGGCC GCCAAGCTGG AGGTCCCGGC AGAGAGATCT 
CCTCGCAGAC GGAGCATCTC TGGGACCAGC ACATCTGAGA AAACCAACTC CATGGATGCT 
GCAAATACCT CTCCTTTCAA AGTATCGGGC TTCTTCAGTA AACGTCTGAA AGGGTCCATC 
AAGAGGACAA AGAGTCAGTC AAAGCTGGAC AGGAACACAA GTTTCCGCCT CCCATCCCTC 
CGCAATGCTG ATGACAGGTC ACGTGGGCTG CCGAAGCTGA AGGAGTCCCG CTCTCATGAG 
TCCCTGCTGA GCCCCGGCAG CGCAGTGGAA GCCCTGGACC TTGGGGCAGA AGAAAAGGTC 
TTTGTAAAGC CACTGCACAG CAGCATCCTC GGACAGGACT TCTGCTTTGA GGTAACCTAC 
TCCAGCGGCA GTAAATGCTT CAGCTGTTCC TCTGCTGCAG AGAGGGATAA GTGGATGGAA 
AACCTACGCA GAACAGTGCA GCCGAATAAG GACAACTGCC GTCGAGCTGA AAATGTGCTC 
CGTCTCTGGA TCATTGAGGC AAAGGACCTG GCCCCCAAGA AGAAATACTT CTGTGAGCTG 
TGCCTTGATG ACACTCTCTT TGCCCGCACC ACCAGCAAAA CGAAAGCAGA TAACATTTTC 
TGGGGGGAGC ACTTTGAGTT CTACGGCCTC CCAGCTCTTC ACAGCATCAC GGTTCACATC 
TACAAGGATG TGGAGAAGAA AAAGAAAAAG GACAAAAACA ATTACGTCGG CCTGGTCAAC 
ATTCCCATGG CCACTGTAAC GGGGCGCCAG TTTGTGGAAA AATGGTACCC AGTCAGCACA 
CCTACACCCA ACAAAGGGAA ATCAGGAGGC CCTTCCATTC GCATCAAGTC GCGTTATCAG 
ACCATCACCA TCTTACCCAT GGAGCAATAC AAGGAGTTTG CAGAGTTTGT TACCAGCAAC 
TACACGATGC TGTGCTCTGT GCTGGAGCCA GTCATCAGCG TAAGGAATAA GGAAGAGATG 
GCTTGTGCAA TGGTGCACAT TCTTCAGAGC ACGGGGAGAG CCAAGGACTT CCTAACGGAT 
CTGGTGATGT CTGAGGTGGA TCGCTGCGGG GAACACGATG TCTTGATCTT CAGGGAGAAC 
ACACTTGCTA CCAAAGCTAT TGAGGAATAC CTGAAGTTGG TAGGGCAGAA GTATCTCCAT 
GATGCGCTAG GGGAGTTTAT CAAGGCTTTG TATGAGTCAG ATGAAAACTG TGAAGTGGAC 
CCCAGCAAAT GTTCATCCAG TGAATTAACA GACCATCAGA GCAACCTGAA AATGTGTTGT 
GAGCTGGCCT TCTGCAAGAT TATCAATTCC TACTGCGTGT TCCCTCGTGA GCTGAAGGAG 
GTGTTTGCAT CTTGGAAGCA GCAGTGCCTG AGCAGGGGCA AGCAGGACAT CAGCGAGCGG 
CTGATCAGTG CCTCCCTGTT CCTCCGCTTC CTGTGCCCCG CCATCATGTC CCCCAGCCTC 
TTCAGCCTCA TGCAGGAGTA TCCTGACGAC CGGACATCGC GCACGCTCAC ACTCATTGCT 
AAAGTCATTC AGAATCTCGC CAATTTTGCT AAGTTTGGTA ACAAGGAAGA GTACATGGCT 
TTCATGAACG ACTTTCTGGA ACATGAGTGG GGGGGAATGA AGCGATTTCT CCTGGAGATC 
TCTAATCCAG ATACCATCTC AAACATGCCT GGATTTGAGG GCTATATCGA CCTGGGCAGA 
GAGCTCTCAG TTTTGCATTC CCTCTTATGG GAGGTTGTGT CCCAACTTGA TAAGGCGACC 
GTGGCAAAAC TGGGACCTCT TCCTCGTATT CTTGCCGACA TCACCAAATC AATGACCAAC 
CCTACGCCAA TACAGCAGCA GCTGAGGCGT TTCACTGAGC ACAGCTCTAG TCCAAACGTC 
AGTGGCAGTC TCTCCTCAGG GCTTCAGAAG ATATTCGAGG ACCCCACTGA TGGTGACTTG 
CATAAACTGA AGTCTCCAAC CCAGGAAAAC ATAGATGGCT ATTTCAGGGG CAAGACCTTG 
CTGTTGGTTC AGCAGGCGTC CACCCAGAGC ATGACTTACT CAGATAAGGA CGAAAGAGAG 
AGCATCTTGC CCAACGGACG TAGCATCTCC CTCATGGACC TGCAGGATCC CCATACCGCT 
CACAGCGACC ACGCTTCCAT GATGCACGAC GTGCCTTTGC GCTTGGCCGG CAGCCAGCTC 
TCCATCACCC AGGTGGCCAA CATCAAGCAG CTGTGGGAGA CCCAGAGCAC GCCCCAAAGC 
GCTCCCCAGG TGAGGAGGCC GCTGCACCCG GCTTTGAACC AGCAGGGCAG CCTCCAGCCT 
CTGTCATTCC AGAACCCGGT GTACCACCTC AACAACCCAC CTGCGCCGTT GCCCAAGGCC 
TCCGTGGACT CCAGCCTGGA GAACCTGAGC ACCGCCAGCT CCCAGAGCCG AAGCAACAGC 
GAAGACTTCA AGCTCAGCGG GCCCAGCAAC AGCAGCATGG AGGACTTCAC CAAGCGCAGC 
ACGCAGAGCG AGGACTTCTC TAGGCGGCAC ACGGTCCCGG ACAAGCACGT CCCCATCGCG 
TTGCCCCGCC AGAACAGCAG CAGCCAGGCA CAGATCCGCA AAATGGACCA GGCCGGGTTA 
GGGGCCAGGG CCAAAGCGCC GCAGTCGCTG CCGCACAGCG CGTCCCTGCG CAGCACGGGC 
AGCATGTCGG CCGTGTCGGG GGCCGTGGTG ACTGAGCCCG TCCAGAACGG CAGCCGCTCC 
CGGCAGCAGT CGTCCTCCTC CAGGGAGAGC CCTGTGCCGA AGGTGAGGGC CATTCAGAGG 
CAGCAAACGC AGCAGGTCCA GTCTCCTGTG GACTCAGCCA CAATGTCACC AGTGGAAAGG 
ACAGCAGCGT GGGTTCTCAA TAACGGGCAG TACGAGGAGG TCGAGGAGGA CACAGAGCAG 
AACCCAGATG AAGCCAAGCA TGCTGAGAAG TACGAACAAG AGATAGCAAA GCTGAAGGAA 
CGCTTGAAAG TGTCGAGCCG CCGGCTGGAG GAGTACGAGC GGCGGCTCCT GGTGCAGGAG 
CAGCAAATGC AGAAGCTGCT GATGGAGTAC AAGTCCAGGC TGGAGGACAG CGAGGAGAGA 
CTGCGCAGGC AACAGGAGGA GAAGGACAGC CAGATGAAAA GCATTATCAG CAGGCTCATG 
GCAGTTGAAG AGGAACTGAA GAAAGATCAT GCAGAGATGC AAGCTGTCAT CGATGCAAAG 
CAGAAAATTA TTGATGCACA GGAGAAGCGG ATCGTGTCCC TGGACTCTGC CAACACGCGG 
CTGATGAGTG CCCTGACGCA GGTGAAGGAG CGCTACAGCA TGCAAGTCCG CAATGGCATC 
TCCCCCACCA ACCCCACCAA GCTTTCCATC ACGGAGAATG GTGAATTCAA AAACAGCAGC 
TGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004943303.2
CDS	632..4897
Translation	MSAFKKYYSLSPRTFRRSLSEQDAGTAALKDYNWYHQMRSKKGSKQTALDREKALGAYRWQTHPRLPWRTSRRAHSCSLRGEGNDSSLVKSSASMDATSADDLHQDSTEMLFQARTRSPRVPFNRSLSEPVPHSANRNAKPFLQAVRSVDCEQHSYFVRGIFSTLSSGFSKIINRRGEQPPTEPLEEWKPAGSQIDPCPGNGLCSVCVTDVKGPPTHRLSCGQSPYTETTTWERKYCILTDSQLVLLNREKEVPTEGLQEPQDATKGRCLRRTVSVPSEGQFPEYPPDGAAKLEVPAERSPRRRSISGTSTSEKTNSMDAANTSPFKVSGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNADDRSRGLPKLKESRSHESLLSPGSAVEALDLGAEEKVFVKPLHSSILGQDFCFEVTYSSGSKCFSCSSAAERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYGLPALHSITVHIYKDVEKKKKKDKNNYVGLVNIPMATVTGRQFVEKWYPVSTPTPNKGKSGGPSIRIKSRYQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEEMACAMVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTLATKAIEEYLKLVGQKYLHDALGEFIKALYESDENCEVDPSKCSSSELTDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCLSRGKQDISERLISASLFLRFLCPAIMSPSLFSLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNMPGFEGYIDLGRELSVLHSLLWEVVSQLDKATVAKLGPLPRILADITKSMTNPTPIQQQLRRFTEHSSSPNVSGSLSSGLQKIFEDPTDGDLHKLKSPTQENIDGYFRGKTLLLVQQASTQSMTYSDKDERESILPNGRSISLMDLQDPHTAHSDHASMMHDVPLRLAGSQLSITQVANIKQLWETQSTPQSAPQVRRPLHPALNQQGSLQPLSFQNPVYHLNNPPAPLPKASVDSSLENLSTASSQSRSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTVPDKHVPIALPRQNSSSQAQIRKMDQAGLGARAKAPQSLPHSASLRSTGSMSAVSGAVVTEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEVEEDTEQNPDEAKHAEKYEQEIAKLKERLKVSSRRLEEYERRLLVQEQQMQKLLMEYKSRLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQEKRIVSLDSANTRLMSALTQVKERYSMQVRNGISPTNPTKLSITENGEFKNSSC

Target ORF information:

RefSeq Version	XM_004943246.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004943246.3

ORF Insert Sequence:

ATGAGTGCTT TTAAAAAGTA TTACAGTCTC TCTCCCAGGA CCTTTAGGAG GTCTTTGTCG 
GAGCAAGATG CTGGCACCGC TGCCTTAAAG GACTATAATT GGTACCACCA AATGAGAAGC 
AAGAAGGGGA GCAAACAAAC AGCTTTGGAC CGAGAGAAAG CACTCGGAGC GTACAGGTGG 
CAAACACATC CCCGTCTTCC CTGGAGGACA AGCAGAAGAG CACACTCTTG CAGCCTCAGA 
GGGGAAGGTA ATGATTCGAG TCTTGTCAAA AGTTCTGCAA GCATGGATGC TACCAGCGCC 
GACGATCTCC ACCAAGACTC AACGGAGATG CTCTTCCAGG CAAGGACCAG ATCACCGCGG 
GTTCCCTTCA ACCGCTCCCT GTCTGAACCT GTGCCGCACT CTGCAAACAG GAATGCCAAG 
CCCTTTCTGC AGGCGGTGCG CTCTGTGGAT TGTGAGCAGC ACAGCTATTT TGTCCGTGGC 
ATCTTCTCAA CTCTCTCAAG TGGCTTTTCA AAGATAATAA ATCGAAGGGG TGAGCAGCCC 
CCCACTGAGC CACTGGAGGA GTGGAAACCA GCCGGTAGTC AAATTGACCC GTGCCCAGGT 
AATGGACTCT GTTCTGTTTG TGTTACAGAT GTAAAAGGAC CACCTACGCA CAGGCTGTCC 
TGCGGCCAGT CCCCCTACAC CGAGACGACA ACATGGGAGA GGAAGTACTG CATCCTGACT 
GACAGCCAGC TGGTGCTGCT CAACAGGGAG AAGGAGGTGC CCACCGAGGG GTTGCAGGAG 
CCGCAGGATG CAACCAAAGG CCGCTGTCTG CGCCGCACGG TGAGCGTGCC CTCCGAGGGC 
CAGTTCCCCG AGTACCCACC AGATGGGGCC GCCAAGCTGG AGGTCCCGGC AGAGAGATCT 
CCTCGCAGAC GGAGCATCTC TGGGACCAGC ACATCTGAGA AAACCAACTC CATGGATGCT 
GCAAATACCT CTCCTTTCAA AGTATCGGGC TTCTTCAGTA AACGTCTGAA AGGGTCCATC 
AAGAGGACAA AGAGTCAGTC AAAGCTGGAC AGGAACACAA GTTTCCGCCT CCCATCCCTC 
CGCAATGCTG ATGACAGGTC ACGTGGGCTG CCGAAGCTGA AGGAGTCCCG CTCTCATGAG 
TCCCTGCTGA GCCCCGGCAG CGCAGTGGAA GCCCTGGACC TTGGGGCAGA AGAAAAGGTC 
TTTGTAAAGC CACTGCACAG CAGCATCCTC GGACAGGACT TCTGCTTTGA GGTAACCTAC 
TCCAGCGGCA GTAAATGCTT CAGCTGTTCC TCTGCTGCAG AGAGGGATAA GTGGATGGAA 
AACCTACGCA GAACAGTGCA GCCGAATAAG GACAACTGCC GTCGAGCTGA AAATGTGCTC 
CGTCTCTGGA TCATTGAGGC AAAGGACCTG GCCCCCAAGA AGAAATACTT CTGTGAGCTG 
TGCCTTGATG ACACTCTCTT TGCCCGCACC ACCAGCAAAA CGAAAGCAGA TAACATTTTC 
TGGGGGGAGC ACTTTGAGTT CTACGGCCTC CCAGCTCTTC ACAGCATCAC GGTTCACATC 
TACAAGGATG TGGAGAAGAA AAAGAAAAAG GACAAAAACA ATTACGTCGG CCTGGTCAAC 
ATTCCCATGG CCACTGTAAC GGGGCGCCAG TTTGTGGAAA AATGGTACCC AGTCAGCACA 
CCTACACCCA ACAAAGGGAA ATCAGGAGGC CCTTCCATTC GCATCAAGTC GCGTTATCAG 
ACCATCACCA TCTTACCCAT GGAGCAATAC AAGGAGTTTG CAGAGTTTGT TACCAGCAAC 
TACACGATGC TGTGCTCTGT GCTGGAGCCA GTCATCAGCG TAAGGAATAA GGAAGAGATG 
GCTTGTGCAA TGGTGCACAT TCTTCAGAGC ACGGGGAGAG CCAAGGACTT CCTAACGGAT 
CTGGTGATGT CTGAGGTGGA TCGCTGCGGG GAACACGATG TCTTGATCTT CAGGGAGAAC 
ACACTTGCTA CCAAAGCTAT TGAGGAATAC CTGAAGTTGG TAGGGCAGAA GTATCTCCAT 
GATGCGCTAG GGGAGTTTAT CAAGGCTTTG TATGAGTCAG ATGAAAACTG TGAAGTGGAC 
CCCAGCAAAT GTTCATCCAG TGAATTAACA GACCATCAGA GCAACCTGAA AATGTGTTGT 
GAGCTGGCCT TCTGCAAGAT TATCAATTCC TACTGCGTGT TCCCTCGTGA GCTGAAGGAG 
GTGTTTGCAT CTTGGAAGCA GCAGTGCCTG AGCAGGGGCA AGCAGGACAT CAGCGAGCGG 
CTGATCAGTG CCTCCCTGTT CCTCCGCTTC CTGTGCCCCG CCATCATGTC CCCCAGCCTC 
TTCAGCCTCA TGCAGGAGTA TCCTGACGAC CGGACATCGC GCACGCTCAC ACTCATTGCT 
AAAGTCATTC AGAATCTCGC CAATTTTGCT AAGTTTGGTA ACAAGGAAGA GTACATGGCT 
TTCATGAACG ACTTTCTGGA ACATGAGTGG GGGGGAATGA AGCGATTTCT CCTGGAGATC 
TCTAATCCAG ATACCATCTC AAACATGCCT GGATTTGAGG GCTATATCGA CCTGGGCAGA 
GAGCTCTCAG TTTTGCATTC CCTCTTATGG GAGGTTGTGT CCCAACTTGA TAAGGCGACC 
GTGGCAAAAC TGGGACCTCT TCCTCGTATT CTTGCCGACA TCACCAAATC AATGACCAAC 
CCTACGCCAA TACAGCAGCA GCTGAGGCGT TTCACTGAGC ACAGCTCTAG TCCAAACGTC 
AGTGGCAGTC TCTCCTCAGG GCTTCAGAAG ATATTCGAGG ACCCCACTGA TGGTGACTTG 
CATAAACTGA AGTCTCCAAC CCAGGAAAAC ATAGATGGCT ATTTCAGGGG CAAGACCTTG 
CTGTTGGTTC AGCAGGCGTC CACCCAGAGC ATGACTTACT CAGATAAGGA CGAAAGAGAG 
AGCATCTTGC CCAACGGACG TAGCATCTCC CTCATGGACC TGCAGGATCC CCATACCGCT 
CACAGCGACC ACGCTTCCAT GATGCACGAC GTGCCTTTGC GCTTGGCCGG CAGCCAGCTC 
TCCATCACCC AGGTGGCCAA CATCAAGCAG CTGTGGGAGA CCCAGAGCAC GCCCCAAAGC 
GCTCCCCAGG TGAGGAGGCC GCTGCACCCG GCTTTGAACC AGCAGGGCAG CCTCCAGCCT 
CTGTCATTCC AGAACCCGGT GTACCACCTC AACAACCCAC CTGCGCCGTT GCCCAAGGCC 
TCCGTGGACT CCAGCCTGGA GAACCTGAGC ACCGCCAGCT CCCAGAGCCG AAGCAACAGC 
GAAGACTTCA AGCTCAGCGG GCCCAGCAAC AGCAGCATGG AGGACTTCAC CAAGCGCAGC 
ACGCAGAGCG AGGACTTCTC TAGGCGGCAC ACGGTCCCGG ACAAGCACGT CCCCATCGCG 
TTGCCCCGCC AGAACAGCAG CAGCCAGGCA CAGATCCGCA AAATGGACCA GGCCGGGTTA 
GGGGCCAGGG CCAAAGCGCC GCAGTCGCTG CCGCACAGCG CGTCCCTGCG CAGCACGGGC 
AGCATGTCGG CCGTGTCGGG GGCCGTGGTG ACTGAGCCCG TCCAGAACGG CAGCCGCTCC 
CGGCAGCAGT CGTCCTCCTC CAGGGAGAGC CCTGTGCCGA AGGTGAGGGC CATTCAGAGG 
CAGCAAACGC AGCAGGTCCA GTCTCCTGTG GACTCAGCCA CAATGTCACC AGTGGAAAGG 
ACAGCAGCGT GGGTTCTCAA TAACGGGCAG TACGAGGAGG TCGAGGAGGA CACAGAGCAG 
AACCCAGATG AAGCCAAGCA TGCTGAGAAG TACGAACAAG AGATAGCAAA GCTGAAGGAA 
CGCTTGAAAG TGTCGAGCCG CCGGCTGGAG GAGTACGAGC GGCGGCTCCT GGTGCAGGAG 
CAGCAAATGC AGAAGCTGCT GATGGAGTAC AAGTCCAGGC TGGAGGACAG CGAGGAGAGA 
CTGCGCAGGC AACAGGAGGA GAAGGACAGC CAGATGAAAA GCATTATCAG CAGGCTCATG 
GCAGTTGAAG AGGAACTGAA GAAAGATCAT GCAGAGATGC AAGCTGTCAT CGATGCAAAG 
CAGAAAATTA TTGATGCACA GGAGAAGCGG ATCGTGTCCC TGGACTCTGC CAACACGCGG 
CTGATGAGTG CCCTGACGCA GGTGAAGGAG CGCTACAGCA TGCAAGTCCG CAATGGCATC 
TCCCCCACCA ACCCCACCAA GCTTTCCATC ACGGAGAATG GTGAATTCAA AAACAGCAGC 
TGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_015290512.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015145998.1 Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OGa152365
Clone ID Related Accession (Same CDS sequence)	XM_015290512.2
Accession Version	XM_015290512.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4266bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	ras GTPase-activating protein nGAP isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006095.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015290512.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGCTT TTAAAAAGTA TTACAGTCTC TCTCCCAGGA CCTTTAGGAG GTCTTTGTCG 
GAGCAAGATG CTGGCACCGC TGCCTTAAAG GACTATAATT GGTACCACCA AATGAGAAGC 
AAGAAGGGGA GCAAACAAAC AGCTTTGGAC CGAGAGAAAG CACTCGGAGC GTACAGGTGG 
CAAACACATC CCCGTCTTCC CTGGAGGACA AGCAGAAGAG CACACTCTTG CAGCCTCAGA 
GGGGAAGGTA ATGATTCGAG TCTTGTCAAA AGTTCTGCAA GCATGGATGC TACCAGCGCC 
GACGATCTCC ACCAAGACTC AACGGAGATG CTCTTCCAGG CAAGGACCAG ATCACCGCGG 
GTTCCCTTCA ACCGCTCCCT GTCTGAACCT GTGCCGCACT CTGCAAACAG GAATGCCAAG 
CCCTTTCTGC AGGCGGTGCG CTCTGTGGAT TGTGAGCAGC ACAGCTATTT TGTCCGTGGC 
ATCTTCTCAA CTCTCTCAAG TGGCTTTTCA AAGATAATAA ATCGAAGGGG TGAGCAGCCC 
CCCACTGAGC CACTGGAGGA GTGGAAACCA GCCGGTAGTC AAATTGACCC GTGCCCAGAT 
GTAAAAGGAC CACCTACGCA CAGGCTGTCC TGCGGCCAGT CCCCCTACAC CGAGACGACA 
ACATGGGAGA GGAAGTACTG CATCCTGACT GACAGCCAGC TGGTGCTGCT CAACAGGGAG 
AAGGAGGTGC CCACCGAGGG GTTGCAGGAG CCGCAGGATG CAACCAAAGG CCGCTGTCTG 
CGCCGCACGG TGAGCGTGCC CTCCGAGGGC CAGTTCCCCG AGTACCCACC AGATGGGGCC 
GCCAAGCTGG AGGTCCCGGC AGAGAGATCT CCTCGCAGAC GGAGCATCTC TGGGACCAGC 
ACATCTGAGA AAACCAACTC CATGGATGCT GCAAATACCT CTCCTTTCAA AGTATCGGGC 
TTCTTCAGTA AACGTCTGAA AGGGTCCATC AAGAGGACAA AGAGTCAGTC AAAGCTGGAC 
AGGAACACAA GTTTCCGCCT CCCATCCCTC CGCAATGCTG ATGACAGGTC ACGTGGGCTG 
CCGAAGCTGA AGGAGTCCCG CTCTCATGAG TCCCTGCTGA GCCCCGGCAG CGCAGTGGAA 
GCCCTGGACC TTGGGGCAGA AGAAAAGGTC TTTGTAAAGC CACTGCACAG CAGCATCCTC 
GGACAGGACT TCTGCTTTGA GGTAACCTAC TCCAGCGGCA GTAAATGCTT CAGCTGTTCC 
TCTGCTGCAG AGAGGGATAA GTGGATGGAA AACCTACGCA GAACAGTGCA GCCGAATAAG 
GACAACTGCC GTCGAGCTGA AAATGTGCTC CGTCTCTGGA TCATTGAGGC AAAGGACCTG 
GCCCCCAAGA AGAAATACTT CTGTGAGCTG TGCCTTGATG ACACTCTCTT TGCCCGCACC 
ACCAGCAAAA CGAAAGCAGA TAACATTTTC TGGGGGGAGC ACTTTGAGTT CTACGGCCTC 
CCAGCTCTTC ACAGCATCAC GGTTCACATC TACAAGGATG TGGAGAAGAA AAAGAAAAAG 
GACAAAAACA ATTACGTCGG CCTGGTCAAC ATTCCCATGG CCACTGTAAC GGGGCGCCAG 
TTTGTGGAAA AATGGTACCC AGTCAGCACA CCTACACCCA ACAAAGGGAA ATCAGGAGGC 
CCTTCCATTC GCATCAAGTC GCGTTATCAG ACCATCACCA TCTTACCCAT GGAGCAATAC 
AAGGAGTTTG CAGAGTTTGT TACCAGCAAC TACACGATGC TGTGCTCTGT GCTGGAGCCA 
GTCATCAGCG TAAGGAATAA GGAAGAGATG GCTTGTGCAA TGGTGCACAT TCTTCAGAGC 
ACGGGGAGAG CCAAGGACTT CCTAACGGAT CTGGTGATGT CTGAGGTGGA TCGCTGCGGG 
GAACACGATG TCTTGATCTT CAGGGAGAAC ACACTTGCTA CCAAAGCTAT TGAGGAATAC 
CTGAAGTTGG TAGGGCAGAA GTATCTCCAT GATGCGCTAG GGGAGTTTAT CAAGGCTTTG 
TATGAGTCAG ATGAAAACTG TGAAGTGGAC CCCAGCAAAT GTTCATCCAG TGAATTAACA 
GACCATCAGA GCAACCTGAA AATGTGTTGT GAGCTGGCCT TCTGCAAGAT TATCAATTCC 
TACTGCGTGT TCCCTCGTGA GCTGAAGGAG GTGTTTGCAT CTTGGAAGCA GCAGTGCCTG 
AGCAGGGGCA AGCAGGACAT CAGCGAGCGG CTGATCAGTG CCTCCCTGTT CCTCCGCTTC 
CTGTGCCCCG CCATCATGTC CCCCAGCCTC TTCAGCCTCA TGCAGGAGTA TCCTGACGAC 
CGGACATCGC GCACGCTCAC ACTCATTGCT AAAGTCATTC AGAATCTCGC CAATTTTGCT 
AAGTTTGGTA ACAAGGAAGA GTACATGGCT TTCATGAACG ACTTTCTGGA ACATGAGTGG 
GGGGGAATGA AGCGATTTCT CCTGGAGATC TCTAATCCAG ATACCATCTC AAACATGCCT 
GGATTTGAGG GCTATATCGA CCTGGGCAGA GAGCTCTCAG TTTTGCATTC CCTCTTATGG 
GAGGTTGTGT CCCAACTTGA TAAGGGTGAA AATTCCTTCC TACAGGCGAC CGTGGCAAAA 
CTGGGACCTC TTCCTCGTAT TCTTGCCGAC ATCACCAAAT CAATGACCAA CCCTACGCCA 
ATACAGCAGC AGCTGAGGCG TTTCACTGAG CACAGCTCTA GTCCAAACGT CAGTGGCAGT 
CTCTCCTCAG GGCTTCAGAA GATATTCGAG GACCCCACTG ATGGTGACTT GCATAAACTG 
AAGTCTCCAA CCCAGGAAAA CATAGATGGC TATTTCAGGG GCAAGACCTT GCTGTTGGTT 
CAGCAGGCGT CCACCCAGAG CATGACTTAC TCAGATAAGG ACGAAAGAGA GAGCATCTTG 
CCCAACGGAC GTAGCATCTC CCTCATGGAC CTGCAGGATC CCCATACCGC TCACAGCGAC 
CACGCTTCCA TGATGCACGA CGTGCCTTTG CGCTTGGCCG GCAGCCAGCT CTCCATCACC 
CAGGTGGCCA ACATCAAGCA GCTGTGGGAG ACCCAGAGCA CGCCCCAAAG CGCTCCCCAG 
GTGAGGAGGC CGCTGCACCC GGCTTTGAAC CAGCAGGGCA GCCTCCAGCC TCTGTCATTC 
CAGAACCCGG TGTACCACCT CAACAACCCA CCTGCGCCGT TGCCCAAGGC CTCCGTGGAC 
TCCAGCCTGG AGAACCTGAG CACCGCCAGC TCCCAGAGCC GAAGCAACAG CGAAGACTTC 
AAGCTCAGCG GGCCCAGCAA CAGCAGCATG GAGGACTTCA CCAAGCGCAG CACGCAGAGC 
GAGGACTTCT CTAGGCGGCA CACGGTCCCG GACAAGCACG TCCCCATCGC GTTGCCCCGC 
CAGAACAGCA GCAGCCAGGC ACAGATCCGC AAAATGGACC AGGCCGGGTT AGGGGCCAGG 
GCCAAAGCGC CGCAGTCGCT GCCGCACAGC GCGTCCCTGC GCAGCACGGG CAGCATGTCG 
GCCGTGTCGG GGGCCGTGGT GACTGAGCCC GTCCAGAACG GCAGCCGCTC CCGGCAGCAG 
TCGTCCTCCT CCAGGGAGAG CCCTGTGCCG AAGGTGAGGG CCATTCAGAG GCAGCAAACG 
CAGCAGGTCC AGTCTCCTGT GGACTCAGCC ACAATGTCAC CAGTGGAAAG GACAGCAGCG 
TGGGTTCTCA ATAACGGGCA GTACGAGGAG GTCGAGGAGG ACACAGAGCA GAACCCAGAT 
GAAGCCAAGC ATGCTGAGAA GTACGAACAA GAGATAGCAA AGCTGAAGGA ACGCTTGAAA 
GTGTCGAGCC GCCGGCTGGA GGAGTACGAG CGGCGGCTCC TGGTGCAGGA GCAGCAAATG 
CAGAAGCTGC TGATGGAGTA CAAGTCCAGG CTGGAGGACA GCGAGGAGAG ACTGCGCAGG 
CAACAGGAGG AGAAGGACAG CCAGATGAAA AGCATTATCA GCAGGCTCAT GGCAGTTGAA 
GAGGAACTGA AGAAAGATCA TGCAGAGATG CAAGCTGTCA TCGATGCAAA GCAGAAAATT 
ATTGATGCAC AGGTCATTAA TGGGAATGAG ATAATTCAAA CAGGAGAAGC GGATCGTGTC 
CCTGGACTCT GCCAACACGC GGCTGATGAG TGCCCTGACG CAGGTGAAGG AGCGCTACAG 
CATGCAAGTC CGCAATGGCA TCTCCCCCAC CAACCCCACC AAGCTTTCCA TCACGGAGAA 
TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015145998.1
CDS	635..4900
Translation	MSAFKKYYSLSPRTFRRSLSEQDAGTAALKDYNWYHQMRSKKGSKQTALDREKALGAYRWQTHPRLPWRTSRRAHSCSLRGEGNDSSLVKSSASMDATSADDLHQDSTEMLFQARTRSPRVPFNRSLSEPVPHSANRNAKPFLQAVRSVDCEQHSYFVRGIFSTLSSGFSKIINRRGEQPPTEPLEEWKPAGSQIDPCPDVKGPPTHRLSCGQSPYTETTTWERKYCILTDSQLVLLNREKEVPTEGLQEPQDATKGRCLRRTVSVPSEGQFPEYPPDGAAKLEVPAERSPRRRSISGTSTSEKTNSMDAANTSPFKVSGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNADDRSRGLPKLKESRSHESLLSPGSAVEALDLGAEEKVFVKPLHSSILGQDFCFEVTYSSGSKCFSCSSAAERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYGLPALHSITVHIYKDVEKKKKKDKNNYVGLVNIPMATVTGRQFVEKWYPVSTPTPNKGKSGGPSIRIKSRYQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEEMACAMVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTLATKAIEEYLKLVGQKYLHDALGEFIKALYESDENCEVDPSKCSSSELTDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCLSRGKQDISERLISASLFLRFLCPAIMSPSLFSLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNMPGFEGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVAKLGPLPRILADITKSMTNPTPIQQQLRRFTEHSSSPNVSGSLSSGLQKIFEDPTDGDLHKLKSPTQENIDGYFRGKTLLLVQQASTQSMTYSDKDERESILPNGRSISLMDLQDPHTAHSDHASMMHDVPLRLAGSQLSITQVANIKQLWETQSTPQSAPQVRRPLHPALNQQGSLQPLSFQNPVYHLNNPPAPLPKASVDSSLENLSTASSQSRSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTVPDKHVPIALPRQNSSSQAQIRKMDQAGLGARAKAPQSLPHSASLRSTGSMSAVSGAVVTEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEVEEDTEQNPDEAKHAEKYEQEIAKLKERLKVSSRRLEEYERRLLVQEQQMQKLLMEYKSRLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQVINGNEIIQTGEADRVPGLCQHAADECPDAGEGALQHASPQWHLPHQPHQAFHHGEW

Target ORF information:

RefSeq Version	XM_015290512.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015290512.2

ORF Insert Sequence:

ATGAGTGCTT TTAAAAAGTA TTACAGTCTC TCTCCCAGGA CCTTTAGGAG GTCTTTGTCG 
GAGCAAGATG CTGGCACCGC TGCCTTAAAG GACTATAATT GGTACCACCA AATGAGAAGC 
AAGAAGGGGA GCAAACAAAC AGCTTTGGAC CGAGAGAAAG CACTCGGAGC GTACAGGTGG 
CAAACACATC CCCGTCTTCC CTGGAGGACA AGCAGAAGAG CACACTCTTG CAGCCTCAGA 
GGGGAAGGTA ATGATTCGAG TCTTGTCAAA AGTTCTGCAA GCATGGATGC TACCAGCGCC 
GACGATCTCC ACCAAGACTC AACGGAGATG CTCTTCCAGG CAAGGACCAG ATCACCGCGG 
GTTCCCTTCA ACCGCTCCCT GTCTGAACCT GTGCCGCACT CTGCAAACAG GAATGCCAAG 
CCCTTTCTGC AGGCGGTGCG CTCTGTGGAT TGTGAGCAGC ACAGCTATTT TGTCCGTGGC 
ATCTTCTCAA CTCTCTCAAG TGGCTTTTCA AAGATAATAA ATCGAAGGGG TGAGCAGCCC 
CCCACTGAGC CACTGGAGGA GTGGAAACCA GCCGGTAGTC AAATTGACCC GTGCCCAGAT 
GTAAAAGGAC CACCTACGCA CAGGCTGTCC TGCGGCCAGT CCCCCTACAC CGAGACGACA 
ACATGGGAGA GGAAGTACTG CATCCTGACT GACAGCCAGC TGGTGCTGCT CAACAGGGAG 
AAGGAGGTGC CCACCGAGGG GTTGCAGGAG CCGCAGGATG CAACCAAAGG CCGCTGTCTG 
CGCCGCACGG TGAGCGTGCC CTCCGAGGGC CAGTTCCCCG AGTACCCACC AGATGGGGCC 
GCCAAGCTGG AGGTCCCGGC AGAGAGATCT CCTCGCAGAC GGAGCATCTC TGGGACCAGC 
ACATCTGAGA AAACCAACTC CATGGATGCT GCAAATACCT CTCCTTTCAA AGTATCGGGC 
TTCTTCAGTA AACGTCTGAA AGGGTCCATC AAGAGGACAA AGAGTCAGTC AAAGCTGGAC 
AGGAACACAA GTTTCCGCCT CCCATCCCTC CGCAATGCTG ATGACAGGTC ACGTGGGCTG 
CCGAAGCTGA AGGAGTCCCG CTCTCATGAG TCCCTGCTGA GCCCCGGCAG CGCAGTGGAA 
GCCCTGGACC TTGGGGCAGA AGAAAAGGTC TTTGTAAAGC CACTGCACAG CAGCATCCTC 
GGACAGGACT TCTGCTTTGA GGTAACCTAC TCCAGCGGCA GTAAATGCTT CAGCTGTTCC 
TCTGCTGCAG AGAGGGATAA GTGGATGGAA AACCTACGCA GAACAGTGCA GCCGAATAAG 
GACAACTGCC GTCGAGCTGA AAATGTGCTC CGTCTCTGGA TCATTGAGGC AAAGGACCTG 
GCCCCCAAGA AGAAATACTT CTGTGAGCTG TGCCTTGATG ACACTCTCTT TGCCCGCACC 
ACCAGCAAAA CGAAAGCAGA TAACATTTTC TGGGGGGAGC ACTTTGAGTT CTACGGCCTC 
CCAGCTCTTC ACAGCATCAC GGTTCACATC TACAAGGATG TGGAGAAGAA AAAGAAAAAG 
GACAAAAACA ATTACGTCGG CCTGGTCAAC ATTCCCATGG CCACTGTAAC GGGGCGCCAG 
TTTGTGGAAA AATGGTACCC AGTCAGCACA CCTACACCCA ACAAAGGGAA ATCAGGAGGC 
CCTTCCATTC GCATCAAGTC GCGTTATCAG ACCATCACCA TCTTACCCAT GGAGCAATAC 
AAGGAGTTTG CAGAGTTTGT TACCAGCAAC TACACGATGC TGTGCTCTGT GCTGGAGCCA 
GTCATCAGCG TAAGGAATAA GGAAGAGATG GCTTGTGCAA TGGTGCACAT TCTTCAGAGC 
ACGGGGAGAG CCAAGGACTT CCTAACGGAT CTGGTGATGT CTGAGGTGGA TCGCTGCGGG 
GAACACGATG TCTTGATCTT CAGGGAGAAC ACACTTGCTA CCAAAGCTAT TGAGGAATAC 
CTGAAGTTGG TAGGGCAGAA GTATCTCCAT GATGCGCTAG GGGAGTTTAT CAAGGCTTTG 
TATGAGTCAG ATGAAAACTG TGAAGTGGAC CCCAGCAAAT GTTCATCCAG TGAATTAACA 
GACCATCAGA GCAACCTGAA AATGTGTTGT GAGCTGGCCT TCTGCAAGAT TATCAATTCC 
TACTGCGTGT TCCCTCGTGA GCTGAAGGAG GTGTTTGCAT CTTGGAAGCA GCAGTGCCTG 
AGCAGGGGCA AGCAGGACAT CAGCGAGCGG CTGATCAGTG CCTCCCTGTT CCTCCGCTTC 
CTGTGCCCCG CCATCATGTC CCCCAGCCTC TTCAGCCTCA TGCAGGAGTA TCCTGACGAC 
CGGACATCGC GCACGCTCAC ACTCATTGCT AAAGTCATTC AGAATCTCGC CAATTTTGCT 
AAGTTTGGTA ACAAGGAAGA GTACATGGCT TTCATGAACG ACTTTCTGGA ACATGAGTGG 
GGGGGAATGA AGCGATTTCT CCTGGAGATC TCTAATCCAG ATACCATCTC AAACATGCCT 
GGATTTGAGG GCTATATCGA CCTGGGCAGA GAGCTCTCAG TTTTGCATTC CCTCTTATGG 
GAGGTTGTGT CCCAACTTGA TAAGGGTGAA AATTCCTTCC TACAGGCGAC CGTGGCAAAA 
CTGGGACCTC TTCCTCGTAT TCTTGCCGAC ATCACCAAAT CAATGACCAA CCCTACGCCA 
ATACAGCAGC AGCTGAGGCG TTTCACTGAG CACAGCTCTA GTCCAAACGT CAGTGGCAGT 
CTCTCCTCAG GGCTTCAGAA GATATTCGAG GACCCCACTG ATGGTGACTT GCATAAACTG 
AAGTCTCCAA CCCAGGAAAA CATAGATGGC TATTTCAGGG GCAAGACCTT GCTGTTGGTT 
CAGCAGGCGT CCACCCAGAG CATGACTTAC TCAGATAAGG ACGAAAGAGA GAGCATCTTG 
CCCAACGGAC GTAGCATCTC CCTCATGGAC CTGCAGGATC CCCATACCGC TCACAGCGAC 
CACGCTTCCA TGATGCACGA CGTGCCTTTG CGCTTGGCCG GCAGCCAGCT CTCCATCACC 
CAGGTGGCCA ACATCAAGCA GCTGTGGGAG ACCCAGAGCA CGCCCCAAAG CGCTCCCCAG 
GTGAGGAGGC CGCTGCACCC GGCTTTGAAC CAGCAGGGCA GCCTCCAGCC TCTGTCATTC 
CAGAACCCGG TGTACCACCT CAACAACCCA CCTGCGCCGT TGCCCAAGGC CTCCGTGGAC 
TCCAGCCTGG AGAACCTGAG CACCGCCAGC TCCCAGAGCC GAAGCAACAG CGAAGACTTC 
AAGCTCAGCG GGCCCAGCAA CAGCAGCATG GAGGACTTCA CCAAGCGCAG CACGCAGAGC 
GAGGACTTCT CTAGGCGGCA CACGGTCCCG GACAAGCACG TCCCCATCGC GTTGCCCCGC 
CAGAACAGCA GCAGCCAGGC ACAGATCCGC AAAATGGACC AGGCCGGGTT AGGGGCCAGG 
GCCAAAGCGC CGCAGTCGCT GCCGCACAGC GCGTCCCTGC GCAGCACGGG CAGCATGTCG 
GCCGTGTCGG GGGCCGTGGT GACTGAGCCC GTCCAGAACG GCAGCCGCTC CCGGCAGCAG 
TCGTCCTCCT CCAGGGAGAG CCCTGTGCCG AAGGTGAGGG CCATTCAGAG GCAGCAAACG 
CAGCAGGTCC AGTCTCCTGT GGACTCAGCC ACAATGTCAC CAGTGGAAAG GACAGCAGCG 
TGGGTTCTCA ATAACGGGCA GTACGAGGAG GTCGAGGAGG ACACAGAGCA GAACCCAGAT 
GAAGCCAAGC ATGCTGAGAA GTACGAACAA GAGATAGCAA AGCTGAAGGA ACGCTTGAAA 
GTGTCGAGCC GCCGGCTGGA GGAGTACGAG CGGCGGCTCC TGGTGCAGGA GCAGCAAATG 
CAGAAGCTGC TGATGGAGTA CAAGTCCAGG CTGGAGGACA GCGAGGAGAG ACTGCGCAGG 
CAACAGGAGG AGAAGGACAG CCAGATGAAA AGCATTATCA GCAGGCTCAT GGCAGTTGAA 
GAGGAACTGA AGAAAGATCA TGCAGAGATG CAAGCTGTCA TCGATGCAAA GCAGAAAATT 
ATTGATGCAC AGGTCATTAA TGGGAATGAG ATAATTCAAA CAGGAGAAGC GGATCGTGTC 
CCTGGACTCT GCCAACACGC GGCTGATGAG TGCCCTGACG CAGGTGAAGG AGCGCTACAG 
CATGCAAGTC CGCAATGGCA TCTCCCCCAC CAACCCCACC AAGCTTTCCA TCACGGAGAA 
TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_015290513.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015145999.1 Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OGa152366
Clone ID Related Accession (Same CDS sequence)	XM_015290513.2
Accession Version	XM_015290513.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3828bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	ras GTPase-activating protein nGAP isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006095.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015290513.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCGG CCCCCGCGGC GAGCGAGCCG GCGGCCTGGG CGGCCGAGGC GCTGGCGGAG 
CCGGAGCTGG GCTCGGACGA GCTGGAGGCG GCGGGCGGAG CGCTGGACCG CGTCCTGCTG 
GACTCGGTGT GCCAGCAGCA GGGCTGGGTC CGCGTCTACG ATGTAAAAGG ACCACCTACG 
CACAGGCTGT CCTGCGGCCA GTCCCCCTAC ACCGAGACGA CAACATGGGA GAGGAAGTAC 
TGCATCCTGA CTGACAGCCA GCTGGTGCTG CTCAACAGGG AGAAGGAGGT GCCCACCGAG 
GGGTTGCAGG AGCCGCAGGA TGCAACCAAA GGCCGCTGTC TGCGCCGCAC GGTGAGCGTG 
CCCTCCGAGG GCCAGTTCCC CGAGTACCCA CCAGATGGGG CCGCCAAGCT GGAGGTCCCG 
GCAGAGAGAT CTCCTCGCAG ACGGAGCATC TCTGGGACCA GCACATCTGA GAAAACCAAC 
TCCATGGATG CTGCAAATAC CTCTCCTTTC AAAGTATCGG GCTTCTTCAG TAAACGTCTG 
AAAGGGTCCA TCAAGAGGAC AAAGAGTCAG TCAAAGCTGG ACAGGAACAC AAGTTTCCGC 
CTCCCATCCC TCCGCAATGC TGATGACAGG TCACGTGGGC TGCCGAAGCT GAAGGAGTCC 
CGCTCTCATG AGTCCCTGCT GAGCCCCGGC AGCGCAGTGG AAGCCCTGGA CCTTGGGGCA 
GAAGAAAAGG TCTTTGTAAA GCCACTGCAC AGCAGCATCC TCGGACAGGA CTTCTGCTTT 
GAGGTAACCT ACTCCAGCGG CAGTAAATGC TTCAGCTGTT CCTCTGCTGC AGAGAGGGAT 
AAGTGGATGG AAAACCTACG CAGAACAGTG CAGCCGAATA AGGACAACTG CCGTCGAGCT 
GAAAATGTGC TCCGTCTCTG GATCATTGAG GCAAAGGACC TGGCCCCCAA GAAGAAATAC 
TTCTGTGAGC TGTGCCTTGA TGACACTCTC TTTGCCCGCA CCACCAGCAA AACGAAAGCA 
GATAACATTT TCTGGGGGGA GCACTTTGAG TTCTACGGCC TCCCAGCTCT TCACAGCATC 
ACGGTTCACA TCTACAAGGA TGTGGAGAAG AAAAAGAAAA AGGACAAAAA CAATTACGTC 
GGCCTGGTCA ACATTCCCAT GGCCACTGTA ACGGGGCGCC AGTTTGTGGA AAAATGGTAC 
CCAGTCAGCA CACCTACACC CAACAAAGGG AAATCAGGAG GCCCTTCCAT TCGCATCAAG 
TCGCGTTATC AGACCATCAC CATCTTACCC ATGGAGCAAT ACAAGGAGTT TGCAGAGTTT 
GTTACCAGCA ACTACACGAT GCTGTGCTCT GTGCTGGAGC CAGTCATCAG CGTAAGGAAT 
AAGGAAGAGA TGGCTTGTGC AATGGTGCAC ATTCTTCAGA GCACGGGGAG AGCCAAGGAC 
TTCCTAACGG ATCTGGTGAT GTCTGAGGTG GATCGCTGCG GGGAACACGA TGTCTTGATC 
TTCAGGGAGA ACACACTTGC TACCAAAGCT ATTGAGGAAT ACCTGAAGTT GGTAGGGCAG 
AAGTATCTCC ATGATGCGCT AGGGGAGTTT ATCAAGGCTT TGTATGAGTC AGATGAAAAC 
TGTGAAGTGG ACCCCAGCAA ATGTTCATCC AGTGAATTAA CAGACCATCA GAGCAACCTG 
AAAATGTGTT GTGAGCTGGC CTTCTGCAAG ATTATCAATT CCTACTGCGT GTTCCCTCGT 
GAGCTGAAGG AGGTGTTTGC ATCTTGGAAG CAGCAGTGCC TGAGCAGGGG CAAGCAGGAC 
ATCAGCGAGC GGCTGATCAG TGCCTCCCTG TTCCTCCGCT TCCTGTGCCC CGCCATCATG 
TCCCCCAGCC TCTTCAGCCT CATGCAGGAG TATCCTGACG ACCGGACATC GCGCACGCTC 
ACACTCATTG CTAAAGTCAT TCAGAATCTC GCCAATTTTG CTAAGTTTGG TAACAAGGAA 
GAGTACATGG CTTTCATGAA CGACTTTCTG GAACATGAGT GGGGGGGAAT GAAGCGATTT 
CTCCTGGAGA TCTCTAATCC AGATACCATC TCAAACATGC CTGGATTTGA GGGCTATATC 
GACCTGGGCA GAGAGCTCTC AGTTTTGCAT TCCCTCTTAT GGGAGGTTGT GTCCCAACTT 
GATAAGGGTG AAAATTCCTT CCTACAGGCG ACCGTGGCAA AACTGGGACC TCTTCCTCGT 
ATTCTTGCCG ACATCACCAA ATCAATGACC AACCCTACGC CAATACAGCA GCAGCTGAGG 
CGTTTCACTG AGCACAGCTC TAGTCCAAAC GTCAGTGGCA GTCTCTCCTC AGGGCTTCAG 
AAGATATTCG AGGACCCCAC TGATGGTGAC TTGCATAAAC TGAAGTCTCC AACCCAGGAA 
AACATAGATG GCTATTTCAG GGGCAAGACC TTGCTGTTGG TTCAGCAGGC GTCCACCCAG 
AGCATGACTT ACTCAGATAA GGACGAAAGA GAGAGCATCT TGCCCAACGG ACGTAGCATC 
TCCCTCATGG ACCTGCAGGA TCCCCATACC GCTCACAGCG ACCACGCTTC CATGATGCAC 
GACGTGCCTT TGCGCTTGGC CGGCAGCCAG CTCTCCATCA CCCAGGTGGC CAACATCAAG 
CAGCTGTGGG AGACCCAGAG CACGCCCCAA AGCGCTCCCC AGGTGAGGAG GCCGCTGCAC 
CCGGCTTTGA ACCAGCAGGG CAGCCTCCAG CCTCTGTCAT TCCAGAACCC GGTGTACCAC 
CTCAACAACC CACCTGCGCC GTTGCCCAAG GCCTCCGTGG ACTCCAGCCT GGAGAACCTG 
AGCACCGCCA GCTCCCAGAG CCGAAGCAAC AGCGAAGACT TCAAGCTCAG CGGGCCCAGC 
AACAGCAGCA TGGAGGACTT CACCAAGCGC AGCACGCAGA GCGAGGACTT CTCTAGGCGG 
CACACGGTCC CGGACAAGCA CGTCCCCATC GCGTTGCCCC GCCAGAACAG CAGCAGCCAG 
GCACAGATCC GCAAAATGGA CCAGGCCGGG TTAGGGGCCA GGGCCAAAGC GCCGCAGTCG 
CTGCCGCACA GCGCGTCCCT GCGCAGCACG GGCAGCATGT CGGCCGTGTC GGGGGCCGTG 
GTGACTGAGC CCGTCCAGAA CGGCAGCCGC TCCCGGCAGC AGTCGTCCTC CTCCAGGGAG 
AGCCCTGTGC CGAAGGTGAG GGCCATTCAG AGGCAGCAAA CGCAGCAGGT CCAGTCTCCT 
GTGGACTCAG CCACAATGTC ACCAGTGGAA AGGACAGCAG CGTGGGTTCT CAATAACGGG 
CAGTACGAGG AGGTCGAGGA GGACACAGAG CAGAACCCAG ATGAAGCCAA GCATGCTGAG 
AAGTACGAAC AAGAGATAGC AAAGCTGAAG GAACGCTTGA AAGTGTCGAG CCGCCGGCTG 
GAGGAGTACG AGCGGCGGCT CCTGGTGCAG GAGCAGCAAA TGCAGAAGCT GCTGATGGAG 
TACAAGTCCA GGCTGGAGGA CAGCGAGGAG AGACTGCGCA GGCAACAGGA GGAGAAGGAC 
AGCCAGATGA AAAGCATTAT CAGCAGGCTC ATGGCAGTTG AAGAGGAACT GAAGAAAGAT 
CATGCAGAGA TGCAAGCTGT CATCGATGCA AAGCAGAAAA TTATTGATGC ACAGGTCATT 
AATGGGAATG AGATAATTCA AACAGGAGAA GCGGATCGTG TCCCTGGACT CTGCCAACAC 
GCGGCTGATG AGTGCCCTGA CGCAGGTGAA GGAGCGCTAC AGCATGCAAG TCCGCAATGG 
CATCTCCCCC ACCAACCCCA CCAAGCTTTC CATCACGGAG AATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015145999.1
CDS	75..3902
Translation	MEAAPAASEPAAWAAEALAEPELGSDELEAAGGALDRVLLDSVCQQQGWVRVYDVKGPPTHRLSCGQSPYTETTTWERKYCILTDSQLVLLNREKEVPTEGLQEPQDATKGRCLRRTVSVPSEGQFPEYPPDGAAKLEVPAERSPRRRSISGTSTSEKTNSMDAANTSPFKVSGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNADDRSRGLPKLKESRSHESLLSPGSAVEALDLGAEEKVFVKPLHSSILGQDFCFEVTYSSGSKCFSCSSAAERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYGLPALHSITVHIYKDVEKKKKKDKNNYVGLVNIPMATVTGRQFVEKWYPVSTPTPNKGKSGGPSIRIKSRYQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEEMACAMVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTLATKAIEEYLKLVGQKYLHDALGEFIKALYESDENCEVDPSKCSSSELTDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCLSRGKQDISERLISASLFLRFLCPAIMSPSLFSLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNMPGFEGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVAKLGPLPRILADITKSMTNPTPIQQQLRRFTEHSSSPNVSGSLSSGLQKIFEDPTDGDLHKLKSPTQENIDGYFRGKTLLLVQQASTQSMTYSDKDERESILPNGRSISLMDLQDPHTAHSDHASMMHDVPLRLAGSQLSITQVANIKQLWETQSTPQSAPQVRRPLHPALNQQGSLQPLSFQNPVYHLNNPPAPLPKASVDSSLENLSTASSQSRSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTVPDKHVPIALPRQNSSSQAQIRKMDQAGLGARAKAPQSLPHSASLRSTGSMSAVSGAVVTEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEVEEDTEQNPDEAKHAEKYEQEIAKLKERLKVSSRRLEEYERRLLVQEQQMQKLLMEYKSRLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQVINGNEIIQTGEADRVPGLCQHAADECPDAGEGALQHASPQWHLPHQPHQAFHHGEW

Target ORF information:

RefSeq Version	XM_015290513.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015290513.2

ORF Insert Sequence:

ATGGAGGCGG CCCCCGCGGC GAGCGAGCCG GCGGCCTGGG CGGCCGAGGC GCTGGCGGAG 
CCGGAGCTGG GCTCGGACGA GCTGGAGGCG GCGGGCGGAG CGCTGGACCG CGTCCTGCTG 
GACTCGGTGT GCCAGCAGCA GGGCTGGGTC CGCGTCTACG ATGTAAAAGG ACCACCTACG 
CACAGGCTGT CCTGCGGCCA GTCCCCCTAC ACCGAGACGA CAACATGGGA GAGGAAGTAC 
TGCATCCTGA CTGACAGCCA GCTGGTGCTG CTCAACAGGG AGAAGGAGGT GCCCACCGAG 
GGGTTGCAGG AGCCGCAGGA TGCAACCAAA GGCCGCTGTC TGCGCCGCAC GGTGAGCGTG 
CCCTCCGAGG GCCAGTTCCC CGAGTACCCA CCAGATGGGG CCGCCAAGCT GGAGGTCCCG 
GCAGAGAGAT CTCCTCGCAG ACGGAGCATC TCTGGGACCA GCACATCTGA GAAAACCAAC 
TCCATGGATG CTGCAAATAC CTCTCCTTTC AAAGTATCGG GCTTCTTCAG TAAACGTCTG 
AAAGGGTCCA TCAAGAGGAC AAAGAGTCAG TCAAAGCTGG ACAGGAACAC AAGTTTCCGC 
CTCCCATCCC TCCGCAATGC TGATGACAGG TCACGTGGGC TGCCGAAGCT GAAGGAGTCC 
CGCTCTCATG AGTCCCTGCT GAGCCCCGGC AGCGCAGTGG AAGCCCTGGA CCTTGGGGCA 
GAAGAAAAGG TCTTTGTAAA GCCACTGCAC AGCAGCATCC TCGGACAGGA CTTCTGCTTT 
GAGGTAACCT ACTCCAGCGG CAGTAAATGC TTCAGCTGTT CCTCTGCTGC AGAGAGGGAT 
AAGTGGATGG AAAACCTACG CAGAACAGTG CAGCCGAATA AGGACAACTG CCGTCGAGCT 
GAAAATGTGC TCCGTCTCTG GATCATTGAG GCAAAGGACC TGGCCCCCAA GAAGAAATAC 
TTCTGTGAGC TGTGCCTTGA TGACACTCTC TTTGCCCGCA CCACCAGCAA AACGAAAGCA 
GATAACATTT TCTGGGGGGA GCACTTTGAG TTCTACGGCC TCCCAGCTCT TCACAGCATC 
ACGGTTCACA TCTACAAGGA TGTGGAGAAG AAAAAGAAAA AGGACAAAAA CAATTACGTC 
GGCCTGGTCA ACATTCCCAT GGCCACTGTA ACGGGGCGCC AGTTTGTGGA AAAATGGTAC 
CCAGTCAGCA CACCTACACC CAACAAAGGG AAATCAGGAG GCCCTTCCAT TCGCATCAAG 
TCGCGTTATC AGACCATCAC CATCTTACCC ATGGAGCAAT ACAAGGAGTT TGCAGAGTTT 
GTTACCAGCA ACTACACGAT GCTGTGCTCT GTGCTGGAGC CAGTCATCAG CGTAAGGAAT 
AAGGAAGAGA TGGCTTGTGC AATGGTGCAC ATTCTTCAGA GCACGGGGAG AGCCAAGGAC 
TTCCTAACGG ATCTGGTGAT GTCTGAGGTG GATCGCTGCG GGGAACACGA TGTCTTGATC 
TTCAGGGAGA ACACACTTGC TACCAAAGCT ATTGAGGAAT ACCTGAAGTT GGTAGGGCAG 
AAGTATCTCC ATGATGCGCT AGGGGAGTTT ATCAAGGCTT TGTATGAGTC AGATGAAAAC 
TGTGAAGTGG ACCCCAGCAA ATGTTCATCC AGTGAATTAA CAGACCATCA GAGCAACCTG 
AAAATGTGTT GTGAGCTGGC CTTCTGCAAG ATTATCAATT CCTACTGCGT GTTCCCTCGT 
GAGCTGAAGG AGGTGTTTGC ATCTTGGAAG CAGCAGTGCC TGAGCAGGGG CAAGCAGGAC 
ATCAGCGAGC GGCTGATCAG TGCCTCCCTG TTCCTCCGCT TCCTGTGCCC CGCCATCATG 
TCCCCCAGCC TCTTCAGCCT CATGCAGGAG TATCCTGACG ACCGGACATC GCGCACGCTC 
ACACTCATTG CTAAAGTCAT TCAGAATCTC GCCAATTTTG CTAAGTTTGG TAACAAGGAA 
GAGTACATGG CTTTCATGAA CGACTTTCTG GAACATGAGT GGGGGGGAAT GAAGCGATTT 
CTCCTGGAGA TCTCTAATCC AGATACCATC TCAAACATGC CTGGATTTGA GGGCTATATC 
GACCTGGGCA GAGAGCTCTC AGTTTTGCAT TCCCTCTTAT GGGAGGTTGT GTCCCAACTT 
GATAAGGGTG AAAATTCCTT CCTACAGGCG ACCGTGGCAA AACTGGGACC TCTTCCTCGT 
ATTCTTGCCG ACATCACCAA ATCAATGACC AACCCTACGC CAATACAGCA GCAGCTGAGG 
CGTTTCACTG AGCACAGCTC TAGTCCAAAC GTCAGTGGCA GTCTCTCCTC AGGGCTTCAG 
AAGATATTCG AGGACCCCAC TGATGGTGAC TTGCATAAAC TGAAGTCTCC AACCCAGGAA 
AACATAGATG GCTATTTCAG GGGCAAGACC TTGCTGTTGG TTCAGCAGGC GTCCACCCAG 
AGCATGACTT ACTCAGATAA GGACGAAAGA GAGAGCATCT TGCCCAACGG ACGTAGCATC 
TCCCTCATGG ACCTGCAGGA TCCCCATACC GCTCACAGCG ACCACGCTTC CATGATGCAC 
GACGTGCCTT TGCGCTTGGC CGGCAGCCAG CTCTCCATCA CCCAGGTGGC CAACATCAAG 
CAGCTGTGGG AGACCCAGAG CACGCCCCAA AGCGCTCCCC AGGTGAGGAG GCCGCTGCAC 
CCGGCTTTGA ACCAGCAGGG CAGCCTCCAG CCTCTGTCAT TCCAGAACCC GGTGTACCAC 
CTCAACAACC CACCTGCGCC GTTGCCCAAG GCCTCCGTGG ACTCCAGCCT GGAGAACCTG 
AGCACCGCCA GCTCCCAGAG CCGAAGCAAC AGCGAAGACT TCAAGCTCAG CGGGCCCAGC 
AACAGCAGCA TGGAGGACTT CACCAAGCGC AGCACGCAGA GCGAGGACTT CTCTAGGCGG 
CACACGGTCC CGGACAAGCA CGTCCCCATC GCGTTGCCCC GCCAGAACAG CAGCAGCCAG 
GCACAGATCC GCAAAATGGA CCAGGCCGGG TTAGGGGCCA GGGCCAAAGC GCCGCAGTCG 
CTGCCGCACA GCGCGTCCCT GCGCAGCACG GGCAGCATGT CGGCCGTGTC GGGGGCCGTG 
GTGACTGAGC CCGTCCAGAA CGGCAGCCGC TCCCGGCAGC AGTCGTCCTC CTCCAGGGAG 
AGCCCTGTGC CGAAGGTGAG GGCCATTCAG AGGCAGCAAA CGCAGCAGGT CCAGTCTCCT 
GTGGACTCAG CCACAATGTC ACCAGTGGAA AGGACAGCAG CGTGGGTTCT CAATAACGGG 
CAGTACGAGG AGGTCGAGGA GGACACAGAG CAGAACCCAG ATGAAGCCAA GCATGCTGAG 
AAGTACGAAC AAGAGATAGC AAAGCTGAAG GAACGCTTGA AAGTGTCGAG CCGCCGGCTG 
GAGGAGTACG AGCGGCGGCT CCTGGTGCAG GAGCAGCAAA TGCAGAAGCT GCTGATGGAG 
TACAAGTCCA GGCTGGAGGA CAGCGAGGAG AGACTGCGCA GGCAACAGGA GGAGAAGGAC 
AGCCAGATGA AAAGCATTAT CAGCAGGCTC ATGGCAGTTG AAGAGGAACT GAAGAAAGAT 
CATGCAGAGA TGCAAGCTGT CATCGATGCA AAGCAGAAAA TTATTGATGC ACAGGTCATT 
AATGGGAATG AGATAATTCA AACAGGAGAA GCGGATCGTG TCCCTGGACT CTGCCAACAC 
GCGGCTGATG AGTGCCCTGA CGCAGGTGAA GGAGCGCTAC AGCATGCAAG TCCGCAATGG 
CATCTCCCCC ACCAACCCCA CCAAGCTTTC CATCACGGAG AATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_015290514.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015146000.1 Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X9, mRNA.

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CloneID	OGa152367
Clone ID Related Accession (Same CDS sequence)	XM_015290514.2
Accession Version	XM_015290514.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3798bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	ras GTPase-activating protein nGAP isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006095.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015290514.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCGG CCCCCGCGGC GAGCGAGCCG GCGGCCTGGG CGGCCGAGGC GCTGGCGGAG 
CCGGAGCTGG GCTCGGACGA GCTGGAGGCG GCGGGCGGAG CGCTGGACCG CGTCCTGCTG 
GACTCGGTGT GCCAGCAGCA GGGCTGGGTC CGCGTCTACG ATGTAAAAGG ACCACCTACG 
CACAGGCTGT CCTGCGGCCA GTCCCCCTAC ACCGAGACGA CAACATGGGA GAGGAAGTAC 
TGCATCCTGA CTGACAGCCA GCTGGTGCTG CTCAACAGGG AGAAGGAGGT GCCCACCGAG 
GGGTTGCAGG AGCCGCAGGA TGCAACCAAA GGCCGCTGTC TGCGCCGCAC GGTGAGCGTG 
CCCTCCGAGG GCCAGTTCCC CGAGTACCCA CCAGATGGGG CCGCCAAGCT GGAGGTCCCG 
GCAGAGAGAT CTCCTCGCAG ACGGAGCATC TCTGGGACCA GCACATCTGA GAAAACCAAC 
TCCATGGATG CTGCAAATAC CTCTCCTTTC AAAGTATCGG GCTTCTTCAG TAAACGTCTG 
AAAGGGTCCA TCAAGAGGAC AAAGAGTCAG TCAAAGCTGG ACAGGAACAC AAGTTTCCGC 
CTCCCATCCC TCCGCAATGC TGATGACAGG TCACGTGGGC TGCCGAAGCT GAAGGAGTCC 
CGCTCTCATG AGTCCCTGCT GAGCCCCGGC AGCGCAGTGG AAGCCCTGGA CCTTGGGGCA 
GAAGAAAAGG TCTTTGTAAA GCCACTGCAC AGCAGCATCC TCGGACAGGA CTTCTGCTTT 
GAGGTAACCT ACTCCAGCGG CAGTAAATGC TTCAGCTGTT CCTCTGCTGC AGAGAGGGAT 
AAGTGGATGG AAAACCTACG CAGAACAGTG CAGCCGAATA AGGACAACTG CCGTCGAGCT 
GAAAATGTGC TCCGTCTCTG GATCATTGAG GCAAAGGACC TGGCCCCCAA GAAGAAATAC 
TTCTGTGAGC TGTGCCTTGA TGACACTCTC TTTGCCCGCA CCACCAGCAA AACGAAAGCA 
GATAACATTT TCTGGGGGGA GCACTTTGAG TTCTACGGCC TCCCAGCTCT TCACAGCATC 
ACGGTTCACA TCTACAAGGA TGTGGAGAAG AAAAAGAAAA AGGACAAAAA CAATTACGTC 
GGCCTGGTCA ACATTCCCAT GGCCACTGTA ACGGGGCGCC AGTTTGTGGA AAAATGGTAC 
CCAGTCAGCA CACCTACACC CAACAAAGGG AAATCAGGAG GCCCTTCCAT TCGCATCAAG 
TCGCGTTATC AGACCATCAC CATCTTACCC ATGGAGCAAT ACAAGGAGTT TGCAGAGTTT 
GTTACCAGCA ACTACACGAT GCTGTGCTCT GTGCTGGAGC CAGTCATCAG CGTAAGGAAT 
AAGGAAGAGA TGGCTTGTGC AATGGTGCAC ATTCTTCAGA GCACGGGGAG AGCCAAGGAC 
TTCCTAACGG ATCTGGTGAT GTCTGAGGTG GATCGCTGCG GGGAACACGA TGTCTTGATC 
TTCAGGGAGA ACACACTTGC TACCAAAGCT ATTGAGGAAT ACCTGAAGTT GGTAGGGCAG 
AAGTATCTCC ATGATGCGCT AGGGGAGTTT ATCAAGGCTT TGTATGAGTC AGATGAAAAC 
TGTGAAGTGG ACCCCAGCAA ATGTTCATCC AGTGAATTAA CAGACCATCA GAGCAACCTG 
AAAATGTGTT GTGAGCTGGC CTTCTGCAAG ATTATCAATT CCTACTGCGT GTTCCCTCGT 
GAGCTGAAGG AGGTGTTTGC ATCTTGGAAG CAGCAGTGCC TGAGCAGGGG CAAGCAGGAC 
ATCAGCGAGC GGCTGATCAG TGCCTCCCTG TTCCTCCGCT TCCTGTGCCC CGCCATCATG 
TCCCCCAGCC TCTTCAGCCT CATGCAGGAG TATCCTGACG ACCGGACATC GCGCACGCTC 
ACACTCATTG CTAAAGTCAT TCAGAATCTC GCCAATTTTG CTAAGTTTGG TAACAAGGAA 
GAGTACATGG CTTTCATGAA CGACTTTCTG GAACATGAGT GGGGGGGAAT GAAGCGATTT 
CTCCTGGAGA TCTCTAATCC AGATACCATC TCAAACATGC CTGGATTTGA GGGCTATATC 
GACCTGGGCA GAGAGCTCTC AGTTTTGCAT TCCCTCTTAT GGGAGGTTGT GTCCCAACTT 
GATAAGGCGA CCGTGGCAAA ACTGGGACCT CTTCCTCGTA TTCTTGCCGA CATCACCAAA 
TCAATGACCA ACCCTACGCC AATACAGCAG CAGCTGAGGC GTTTCACTGA GCACAGCTCT 
AGTCCAAACG TCAGTGGCAG TCTCTCCTCA GGGCTTCAGA AGATATTCGA GGACCCCACT 
GATGGTGACT TGCATAAACT GAAGTCTCCA ACCCAGGAAA ACATAGATGG CTATTTCAGG 
GGCAAGACCT TGCTGTTGGT TCAGCAGGCG TCCACCCAGA GCATGACTTA CTCAGATAAG 
GACGAAAGAG AGAGCATCTT GCCCAACGGA CGTAGCATCT CCCTCATGGA CCTGCAGGAT 
CCCCATACCG CTCACAGCGA CCACGCTTCC ATGATGCACG ACGTGCCTTT GCGCTTGGCC 
GGCAGCCAGC TCTCCATCAC CCAGGTGGCC AACATCAAGC AGCTGTGGGA GACCCAGAGC 
ACGCCCCAAA GCGCTCCCCA GGTGAGGAGG CCGCTGCACC CGGCTTTGAA CCAGCAGGGC 
AGCCTCCAGC CTCTGTCATT CCAGAACCCG GTGTACCACC TCAACAACCC ACCTGCGCCG 
TTGCCCAAGG CCTCCGTGGA CTCCAGCCTG GAGAACCTGA GCACCGCCAG CTCCCAGAGC 
CGAAGCAACA GCGAAGACTT CAAGCTCAGC GGGCCCAGCA ACAGCAGCAT GGAGGACTTC 
ACCAAGCGCA GCACGCAGAG CGAGGACTTC TCTAGGCGGC ACACGGTCCC GGACAAGCAC 
GTCCCCATCG CGTTGCCCCG CCAGAACAGC AGCAGCCAGG CACAGATCCG CAAAATGGAC 
CAGGCCGGGT TAGGGGCCAG GGCCAAAGCG CCGCAGTCGC TGCCGCACAG CGCGTCCCTG 
CGCAGCACGG GCAGCATGTC GGCCGTGTCG GGGGCCGTGG TGACTGAGCC CGTCCAGAAC 
GGCAGCCGCT CCCGGCAGCA GTCGTCCTCC TCCAGGGAGA GCCCTGTGCC GAAGGTGAGG 
GCCATTCAGA GGCAGCAAAC GCAGCAGGTC CAGTCTCCTG TGGACTCAGC CACAATGTCA 
CCAGTGGAAA GGACAGCAGC GTGGGTTCTC AATAACGGGC AGTACGAGGA GGTCGAGGAG 
GACACAGAGC AGAACCCAGA TGAAGCCAAG CATGCTGAGA AGTACGAACA AGAGATAGCA 
AAGCTGAAGG AACGCTTGAA AGTGTCGAGC CGCCGGCTGG AGGAGTACGA GCGGCGGCTC 
CTGGTGCAGG AGCAGCAAAT GCAGAAGCTG CTGATGGAGT ACAAGTCCAG GCTGGAGGAC 
AGCGAGGAGA GACTGCGCAG GCAACAGGAG GAGAAGGACA GCCAGATGAA AAGCATTATC 
AGCAGGCTCA TGGCAGTTGA AGAGGAACTG AAGAAAGATC ATGCAGAGAT GCAAGCTGTC 
ATCGATGCAA AGCAGAAAAT TATTGATGCA CAGGAGAAGC GGATCGTGTC CCTGGACTCT 
GCCAACACGC GGCTGATGAG TGCCCTGACG CAGGTGAAGG AGCGCTACAG CATGCAAGTC 
CGCAATGGCA TCTCCCCCAC CAACCCCACC AAGCTTTCCA TCACGGAGAA TGGTGAATTC 
AAAAACAGCA GCTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015146000.1
CDS	74..3871
Translation	MEAAPAASEPAAWAAEALAEPELGSDELEAAGGALDRVLLDSVCQQQGWVRVYDVKGPPTHRLSCGQSPYTETTTWERKYCILTDSQLVLLNREKEVPTEGLQEPQDATKGRCLRRTVSVPSEGQFPEYPPDGAAKLEVPAERSPRRRSISGTSTSEKTNSMDAANTSPFKVSGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNADDRSRGLPKLKESRSHESLLSPGSAVEALDLGAEEKVFVKPLHSSILGQDFCFEVTYSSGSKCFSCSSAAERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYGLPALHSITVHIYKDVEKKKKKDKNNYVGLVNIPMATVTGRQFVEKWYPVSTPTPNKGKSGGPSIRIKSRYQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEEMACAMVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTLATKAIEEYLKLVGQKYLHDALGEFIKALYESDENCEVDPSKCSSSELTDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCLSRGKQDISERLISASLFLRFLCPAIMSPSLFSLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNMPGFEGYIDLGRELSVLHSLLWEVVSQLDKATVAKLGPLPRILADITKSMTNPTPIQQQLRRFTEHSSSPNVSGSLSSGLQKIFEDPTDGDLHKLKSPTQENIDGYFRGKTLLLVQQASTQSMTYSDKDERESILPNGRSISLMDLQDPHTAHSDHASMMHDVPLRLAGSQLSITQVANIKQLWETQSTPQSAPQVRRPLHPALNQQGSLQPLSFQNPVYHLNNPPAPLPKASVDSSLENLSTASSQSRSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTVPDKHVPIALPRQNSSSQAQIRKMDQAGLGARAKAPQSLPHSASLRSTGSMSAVSGAVVTEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEVEEDTEQNPDEAKHAEKYEQEIAKLKERLKVSSRRLEEYERRLLVQEQQMQKLLMEYKSRLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQEKRIVSLDSANTRLMSALTQVKERYSMQVRNGISPTNPTKLSITENGEFKNSSC

Target ORF information:

RefSeq Version	XM_015290514.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015290514.2

ORF Insert Sequence:

ATGGAGGCGG CCCCCGCGGC GAGCGAGCCG GCGGCCTGGG CGGCCGAGGC GCTGGCGGAG 
CCGGAGCTGG GCTCGGACGA GCTGGAGGCG GCGGGCGGAG CGCTGGACCG CGTCCTGCTG 
GACTCGGTGT GCCAGCAGCA GGGCTGGGTC CGCGTCTACG ATGTAAAAGG ACCACCTACG 
CACAGGCTGT CCTGCGGCCA GTCCCCCTAC ACCGAGACGA CAACATGGGA GAGGAAGTAC 
TGCATCCTGA CTGACAGCCA GCTGGTGCTG CTCAACAGGG AGAAGGAGGT GCCCACCGAG 
GGGTTGCAGG AGCCGCAGGA TGCAACCAAA GGCCGCTGTC TGCGCCGCAC GGTGAGCGTG 
CCCTCCGAGG GCCAGTTCCC CGAGTACCCA CCAGATGGGG CCGCCAAGCT GGAGGTCCCG 
GCAGAGAGAT CTCCTCGCAG ACGGAGCATC TCTGGGACCA GCACATCTGA GAAAACCAAC 
TCCATGGATG CTGCAAATAC CTCTCCTTTC AAAGTATCGG GCTTCTTCAG TAAACGTCTG 
AAAGGGTCCA TCAAGAGGAC AAAGAGTCAG TCAAAGCTGG ACAGGAACAC AAGTTTCCGC 
CTCCCATCCC TCCGCAATGC TGATGACAGG TCACGTGGGC TGCCGAAGCT GAAGGAGTCC 
CGCTCTCATG AGTCCCTGCT GAGCCCCGGC AGCGCAGTGG AAGCCCTGGA CCTTGGGGCA 
GAAGAAAAGG TCTTTGTAAA GCCACTGCAC AGCAGCATCC TCGGACAGGA CTTCTGCTTT 
GAGGTAACCT ACTCCAGCGG CAGTAAATGC TTCAGCTGTT CCTCTGCTGC AGAGAGGGAT 
AAGTGGATGG AAAACCTACG CAGAACAGTG CAGCCGAATA AGGACAACTG CCGTCGAGCT 
GAAAATGTGC TCCGTCTCTG GATCATTGAG GCAAAGGACC TGGCCCCCAA GAAGAAATAC 
TTCTGTGAGC TGTGCCTTGA TGACACTCTC TTTGCCCGCA CCACCAGCAA AACGAAAGCA 
GATAACATTT TCTGGGGGGA GCACTTTGAG TTCTACGGCC TCCCAGCTCT TCACAGCATC 
ACGGTTCACA TCTACAAGGA TGTGGAGAAG AAAAAGAAAA AGGACAAAAA CAATTACGTC 
GGCCTGGTCA ACATTCCCAT GGCCACTGTA ACGGGGCGCC AGTTTGTGGA AAAATGGTAC 
CCAGTCAGCA CACCTACACC CAACAAAGGG AAATCAGGAG GCCCTTCCAT TCGCATCAAG 
TCGCGTTATC AGACCATCAC CATCTTACCC ATGGAGCAAT ACAAGGAGTT TGCAGAGTTT 
GTTACCAGCA ACTACACGAT GCTGTGCTCT GTGCTGGAGC CAGTCATCAG CGTAAGGAAT 
AAGGAAGAGA TGGCTTGTGC AATGGTGCAC ATTCTTCAGA GCACGGGGAG AGCCAAGGAC 
TTCCTAACGG ATCTGGTGAT GTCTGAGGTG GATCGCTGCG GGGAACACGA TGTCTTGATC 
TTCAGGGAGA ACACACTTGC TACCAAAGCT ATTGAGGAAT ACCTGAAGTT GGTAGGGCAG 
AAGTATCTCC ATGATGCGCT AGGGGAGTTT ATCAAGGCTT TGTATGAGTC AGATGAAAAC 
TGTGAAGTGG ACCCCAGCAA ATGTTCATCC AGTGAATTAA CAGACCATCA GAGCAACCTG 
AAAATGTGTT GTGAGCTGGC CTTCTGCAAG ATTATCAATT CCTACTGCGT GTTCCCTCGT 
GAGCTGAAGG AGGTGTTTGC ATCTTGGAAG CAGCAGTGCC TGAGCAGGGG CAAGCAGGAC 
ATCAGCGAGC GGCTGATCAG TGCCTCCCTG TTCCTCCGCT TCCTGTGCCC CGCCATCATG 
TCCCCCAGCC TCTTCAGCCT CATGCAGGAG TATCCTGACG ACCGGACATC GCGCACGCTC 
ACACTCATTG CTAAAGTCAT TCAGAATCTC GCCAATTTTG CTAAGTTTGG TAACAAGGAA 
GAGTACATGG CTTTCATGAA CGACTTTCTG GAACATGAGT GGGGGGGAAT GAAGCGATTT 
CTCCTGGAGA TCTCTAATCC AGATACCATC TCAAACATGC CTGGATTTGA GGGCTATATC 
GACCTGGGCA GAGAGCTCTC AGTTTTGCAT TCCCTCTTAT GGGAGGTTGT GTCCCAACTT 
GATAAGGCGA CCGTGGCAAA ACTGGGACCT CTTCCTCGTA TTCTTGCCGA CATCACCAAA 
TCAATGACCA ACCCTACGCC AATACAGCAG CAGCTGAGGC GTTTCACTGA GCACAGCTCT 
AGTCCAAACG TCAGTGGCAG TCTCTCCTCA GGGCTTCAGA AGATATTCGA GGACCCCACT 
GATGGTGACT TGCATAAACT GAAGTCTCCA ACCCAGGAAA ACATAGATGG CTATTTCAGG 
GGCAAGACCT TGCTGTTGGT TCAGCAGGCG TCCACCCAGA GCATGACTTA CTCAGATAAG 
GACGAAAGAG AGAGCATCTT GCCCAACGGA CGTAGCATCT CCCTCATGGA CCTGCAGGAT 
CCCCATACCG CTCACAGCGA CCACGCTTCC ATGATGCACG ACGTGCCTTT GCGCTTGGCC 
GGCAGCCAGC TCTCCATCAC CCAGGTGGCC AACATCAAGC AGCTGTGGGA GACCCAGAGC 
ACGCCCCAAA GCGCTCCCCA GGTGAGGAGG CCGCTGCACC CGGCTTTGAA CCAGCAGGGC 
AGCCTCCAGC CTCTGTCATT CCAGAACCCG GTGTACCACC TCAACAACCC ACCTGCGCCG 
TTGCCCAAGG CCTCCGTGGA CTCCAGCCTG GAGAACCTGA GCACCGCCAG CTCCCAGAGC 
CGAAGCAACA GCGAAGACTT CAAGCTCAGC GGGCCCAGCA ACAGCAGCAT GGAGGACTTC 
ACCAAGCGCA GCACGCAGAG CGAGGACTTC TCTAGGCGGC ACACGGTCCC GGACAAGCAC 
GTCCCCATCG CGTTGCCCCG CCAGAACAGC AGCAGCCAGG CACAGATCCG CAAAATGGAC 
CAGGCCGGGT TAGGGGCCAG GGCCAAAGCG CCGCAGTCGC TGCCGCACAG CGCGTCCCTG 
CGCAGCACGG GCAGCATGTC GGCCGTGTCG GGGGCCGTGG TGACTGAGCC CGTCCAGAAC 
GGCAGCCGCT CCCGGCAGCA GTCGTCCTCC TCCAGGGAGA GCCCTGTGCC GAAGGTGAGG 
GCCATTCAGA GGCAGCAAAC GCAGCAGGTC CAGTCTCCTG TGGACTCAGC CACAATGTCA 
CCAGTGGAAA GGACAGCAGC GTGGGTTCTC AATAACGGGC AGTACGAGGA GGTCGAGGAG 
GACACAGAGC AGAACCCAGA TGAAGCCAAG CATGCTGAGA AGTACGAACA AGAGATAGCA 
AAGCTGAAGG AACGCTTGAA AGTGTCGAGC CGCCGGCTGG AGGAGTACGA GCGGCGGCTC 
CTGGTGCAGG AGCAGCAAAT GCAGAAGCTG CTGATGGAGT ACAAGTCCAG GCTGGAGGAC 
AGCGAGGAGA GACTGCGCAG GCAACAGGAG GAGAAGGACA GCCAGATGAA AAGCATTATC 
AGCAGGCTCA TGGCAGTTGA AGAGGAACTG AAGAAAGATC ATGCAGAGAT GCAAGCTGTC 
ATCGATGCAA AGCAGAAAAT TATTGATGCA CAGGAGAAGC GGATCGTGTC CCTGGACTCT 
GCCAACACGC GGCTGATGAG TGCCCTGACG CAGGTGAAGG AGCGCTACAG CATGCAAGTC 
CGCAATGGCA TCTCCCCCAC CAACCCCACC AAGCTTTCCA TCACGGAGAA TGGTGAATTC 
AAAAACAGCA GCTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_015290515.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015146001.1 Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X10, mRNA.

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CloneID	OGa152368
Clone ID Related Accession (Same CDS sequence)	XM_015290515.2
Accession Version	XM_015290515.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	ras GTPase-activating protein nGAP isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006095.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015290515.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAGCC TGGTGAGGAA GATGCTAGTG CAGACAAAGC CATGGAGCGA TGTAAAAGGA 
CCACCTACGC ACAGGCTGTC CTGCGGCCAG TCCCCCTACA CCGAGACGAC AACATGGGAG 
AGGAAGTACT GCATCCTGAC TGACAGCCAG CTGGTGCTGC TCAACAGGGA GAAGGAGGTG 
CCCACCGAGG GGTTGCAGGA GCCGCAGGAT GCAACCAAAG GCCGCTGTCT GCGCCGCACG 
GTGAGCGTGC CCTCCGAGGG CCAGTTCCCC GAGTACCCAC CAGATGGGGC CGCCAAGCTG 
GAGGTCCCGG CAGAGAGATC TCCTCGCAGA CGGAGCATCT CTGGGACCAG CACATCTGAG 
AAAACCAACT CCATGGATGC TGCAAATACC TCTCCTTTCA AAGTATCGGG CTTCTTCAGT 
AAACGTCTGA AAGGGTCCAT CAAGAGGACA AAGAGTCAGT CAAAGCTGGA CAGGAACACA 
AGTTTCCGCC TCCCATCCCT CCGCAATGCT GATGACAGGT CACGTGGGCT GCCGAAGCTG 
AAGGAGTCCC GCTCTCATGA GTCCCTGCTG AGCCCCGGCA GCGCAGTGGA AGCCCTGGAC 
CTTGGGGCAG AAGAAAAGGT CTTTGTAAAG CCACTGCACA GCAGCATCCT CGGACAGGAC 
TTCTGCTTTG AGGTAACCTA CTCCAGCGGC AGTAAATGCT TCAGCTGTTC CTCTGCTGCA 
GAGAGGGATA AGTGGATGGA AAACCTACGC AGAACAGTGC AGCCGAATAA GGACAACTGC 
CGTCGAGCTG AAAATGTGCT CCGTCTCTGG ATCATTGAGG CAAAGGACCT GGCCCCCAAG 
AAGAAATACT TCTGTGAGCT GTGCCTTGAT GACACTCTCT TTGCCCGCAC CACCAGCAAA 
ACGAAAGCAG ATAACATTTT CTGGGGGGAG CACTTTGAGT TCTACGGCCT CCCAGCTCTT 
CACAGCATCA CGGTTCACAT CTACAAGGAT GTGGAGAAGA AAAAGAAAAA GGACAAAAAC 
AATTACGTCG GCCTGGTCAA CATTCCCATG GCCACTGTAA CGGGGCGCCA GTTTGTGGAA 
AAATGGTACC CAGTCAGCAC ACCTACACCC AACAAAGGGA AATCAGGAGG CCCTTCCATT 
CGCATCAAGT CGCGTTATCA GACCATCACC ATCTTACCCA TGGAGCAATA CAAGGAGTTT 
GCAGAGTTTG TTACCAGCAA CTACACGATG CTGTGCTCTG TGCTGGAGCC AGTCATCAGC 
GTAAGGAATA AGGAAGAGAT GGCTTGTGCA ATGGTGCACA TTCTTCAGAG CACGGGGAGA 
GCCAAGGACT TCCTAACGGA TCTGGTGATG TCTGAGGTGG ATCGCTGCGG GGAACACGAT 
GTCTTGATCT TCAGGGAGAA CACACTTGCT ACCAAAGCTA TTGAGGAATA CCTGAAGTTG 
GTAGGGCAGA AGTATCTCCA TGATGCGCTA GGGGAGTTTA TCAAGGCTTT GTATGAGTCA 
GATGAAAACT GTGAAGTGGA CCCCAGCAAA TGTTCATCCA GTGAATTAAC AGACCATCAG 
AGCAACCTGA AAATGTGTTG TGAGCTGGCC TTCTGCAAGA TTATCAATTC CTACTGCGTG 
TTCCCTCGTG AGCTGAAGGA GGTGTTTGCA TCTTGGAAGC AGCAGTGCCT GAGCAGGGGC 
AAGCAGGACA TCAGCGAGCG GCTGATCAGT GCCTCCCTGT TCCTCCGCTT CCTGTGCCCC 
GCCATCATGT CCCCCAGCCT CTTCAGCCTC ATGCAGGAGT ATCCTGACGA CCGGACATCG 
CGCACGCTCA CACTCATTGC TAAAGTCATT CAGAATCTCG CCAATTTTGC TAAGTTTGGT 
AACAAGGAAG AGTACATGGC TTTCATGAAC GACTTTCTGG AACATGAGTG GGGGGGAATG 
AAGCGATTTC TCCTGGAGAT CTCTAATCCA GATACCATCT CAAACATGCC TGGATTTGAG 
GGCTATATCG ACCTGGGCAG AGAGCTCTCA GTTTTGCATT CCCTCTTATG GGAGGTTGTG 
TCCCAACTTG ATAAGGGTGA AAATTCCTTC CTACAGGCGA CCGTGGCAAA ACTGGGACCT 
CTTCCTCGTA TTCTTGCCGA CATCACCAAA TCAATGACCA ACCCTACGCC AATACAGCAG 
CAGCTGAGGC GTTTCACTGA GCACAGCTCT AGTCCAAACG TCAGTGGCAG TCTCTCCTCA 
GGGCTTCAGA AGATATTCGA GGACCCCACT GATGGTGACT TGCATAAACT GAAGTCTCCA 
ACCCAGGAAA ACATAGATGG CTATTTCAGG GGCAAGACCT TGCTGTTGGT TCAGCAGGCG 
TCCACCCAGA GCATGACTTA CTCAGATAAG GACGAAAGAG AGAGCATCTT GCCCAACGGA 
CGTAGCATCT CCCTCATGGA CCTGCAGGAT CCCCATACCG CTCACAGCGA CCACGCTTCC 
ATGATGCACG ACGTGCCTTT GCGCTTGGCC GGCAGCCAGC TCTCCATCAC CCAGGTGGCC 
AACATCAAGC AGCTGTGGGA GACCCAGAGC ACGCCCCAAA GCGCTCCCCA GGTGAGGAGG 
CCGCTGCACC CGGCTTTGAA CCAGCAGGGC AGCCTCCAGC CTCTGTCATT CCAGAACCCG 
GTGTACCACC TCAACAACCC ACCTGCGCCG TTGCCCAAGG CCTCCGTGGA CTCCAGCCTG 
GAGAACCTGA GCACCGCCAG CTCCCAGAGC CGAAGCAACA GCGAAGACTT CAAGCTCAGC 
GGGCCCAGCA ACAGCAGCAT GGAGGACTTC ACCAAGCGCA GCACGCAGAG CGAGGACTTC 
TCTAGGCGGC ACACGGTCCC GGACAAGCAC GTCCCCATCG CGTTGCCCCG CCAGAACAGC 
AGCAGCCAGG CACAGATCCG CAAAATGGAC CAGGCCGGGT TAGGGGCCAG GGCCAAAGCG 
CCGCAGTCGC TGCCGCACAG CGCGTCCCTG CGCAGCACGG GCAGCATGTC GGCCGTGTCG 
GGGGCCGTGG TGACTGAGCC CGTCCAGAAC GGCAGCCGCT CCCGGCAGCA GTCGTCCTCC 
TCCAGGGAGA GCCCTGTGCC GAAGGTGAGG GCCATTCAGA GGCAGCAAAC GCAGCAGGTC 
CAGTCTCCTG TGGACTCAGC CACAATGTCA CCAGTGGAAA GGACAGCAGC GTGGGTTCTC 
AATAACGGGC AGTACGAGGA GGTCGAGGAG GACACAGAGC AGAACCCAGA TGAAGCCAAG 
CATGCTGAGA AGTACGAACA AGAGATAGCA AAGCTGAAGG AACGCTTGAA AGTGTCGAGC 
CGCCGGCTGG AGGAGTACGA GCGGCGGCTC CTGGTGCAGG AGCAGCAAAT GCAGAAGCTG 
CTGATGGAGT ACAAGTCCAG GCTGGAGGAC AGCGAGGAGA GACTGCGCAG GCAACAGGAG 
GAGAAGGACA GCCAGATGAA AAGCATTATC AGCAGGCTCA TGGCAGTTGA AGAGGAACTG 
AAGAAAGATC ATGCAGAGAT GCAAGCTGTC ATCGATGCAA AGCAGAAAAT TATTGATGCA 
CAGGTCATTA ATGGGAATGA GATAATTCAA ACAGGAGAAG CGGATCGTGT CCCTGGACTC 
TGCCAACACG CGGCTGATGA GTGCCCTGAC GCAGGTGAAG GAGCGCTACA GCATGCAAGT 
CCGCAATGGC ATCTCCCCCA CCAACCCCAC CAAGCTTTCC ATCACGGAGA ATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015146001.1
CDS	17170..20886
Translation	MASLVRKMLVQTKPWSDVKGPPTHRLSCGQSPYTETTTWERKYCILTDSQLVLLNREKEVPTEGLQEPQDATKGRCLRRTVSVPSEGQFPEYPPDGAAKLEVPAERSPRRRSISGTSTSEKTNSMDAANTSPFKVSGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNADDRSRGLPKLKESRSHESLLSPGSAVEALDLGAEEKVFVKPLHSSILGQDFCFEVTYSSGSKCFSCSSAAERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYGLPALHSITVHIYKDVEKKKKKDKNNYVGLVNIPMATVTGRQFVEKWYPVSTPTPNKGKSGGPSIRIKSRYQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEEMACAMVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTLATKAIEEYLKLVGQKYLHDALGEFIKALYESDENCEVDPSKCSSSELTDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCLSRGKQDISERLISASLFLRFLCPAIMSPSLFSLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNMPGFEGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVAKLGPLPRILADITKSMTNPTPIQQQLRRFTEHSSSPNVSGSLSSGLQKIFEDPTDGDLHKLKSPTQENIDGYFRGKTLLLVQQASTQSMTYSDKDERESILPNGRSISLMDLQDPHTAHSDHASMMHDVPLRLAGSQLSITQVANIKQLWETQSTPQSAPQVRRPLHPALNQQGSLQPLSFQNPVYHLNNPPAPLPKASVDSSLENLSTASSQSRSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTVPDKHVPIALPRQNSSSQAQIRKMDQAGLGARAKAPQSLPHSASLRSTGSMSAVSGAVVTEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEVEEDTEQNPDEAKHAEKYEQEIAKLKERLKVSSRRLEEYERRLLVQEQQMQKLLMEYKSRLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQVINGNEIIQTGEADRVPGLCQHAADECPDAGEGALQHASPQWHLPHQPHQAFHHGEW

Target ORF information:

RefSeq Version	XM_015290515.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015290515.2

ORF Insert Sequence:

ATGGCAAGCC TGGTGAGGAA GATGCTAGTG CAGACAAAGC CATGGAGCGA TGTAAAAGGA 
CCACCTACGC ACAGGCTGTC CTGCGGCCAG TCCCCCTACA CCGAGACGAC AACATGGGAG 
AGGAAGTACT GCATCCTGAC TGACAGCCAG CTGGTGCTGC TCAACAGGGA GAAGGAGGTG 
CCCACCGAGG GGTTGCAGGA GCCGCAGGAT GCAACCAAAG GCCGCTGTCT GCGCCGCACG 
GTGAGCGTGC CCTCCGAGGG CCAGTTCCCC GAGTACCCAC CAGATGGGGC CGCCAAGCTG 
GAGGTCCCGG CAGAGAGATC TCCTCGCAGA CGGAGCATCT CTGGGACCAG CACATCTGAG 
AAAACCAACT CCATGGATGC TGCAAATACC TCTCCTTTCA AAGTATCGGG CTTCTTCAGT 
AAACGTCTGA AAGGGTCCAT CAAGAGGACA AAGAGTCAGT CAAAGCTGGA CAGGAACACA 
AGTTTCCGCC TCCCATCCCT CCGCAATGCT GATGACAGGT CACGTGGGCT GCCGAAGCTG 
AAGGAGTCCC GCTCTCATGA GTCCCTGCTG AGCCCCGGCA GCGCAGTGGA AGCCCTGGAC 
CTTGGGGCAG AAGAAAAGGT CTTTGTAAAG CCACTGCACA GCAGCATCCT CGGACAGGAC 
TTCTGCTTTG AGGTAACCTA CTCCAGCGGC AGTAAATGCT TCAGCTGTTC CTCTGCTGCA 
GAGAGGGATA AGTGGATGGA AAACCTACGC AGAACAGTGC AGCCGAATAA GGACAACTGC 
CGTCGAGCTG AAAATGTGCT CCGTCTCTGG ATCATTGAGG CAAAGGACCT GGCCCCCAAG 
AAGAAATACT TCTGTGAGCT GTGCCTTGAT GACACTCTCT TTGCCCGCAC CACCAGCAAA 
ACGAAAGCAG ATAACATTTT CTGGGGGGAG CACTTTGAGT TCTACGGCCT CCCAGCTCTT 
CACAGCATCA CGGTTCACAT CTACAAGGAT GTGGAGAAGA AAAAGAAAAA GGACAAAAAC 
AATTACGTCG GCCTGGTCAA CATTCCCATG GCCACTGTAA CGGGGCGCCA GTTTGTGGAA 
AAATGGTACC CAGTCAGCAC ACCTACACCC AACAAAGGGA AATCAGGAGG CCCTTCCATT 
CGCATCAAGT CGCGTTATCA GACCATCACC ATCTTACCCA TGGAGCAATA CAAGGAGTTT 
GCAGAGTTTG TTACCAGCAA CTACACGATG CTGTGCTCTG TGCTGGAGCC AGTCATCAGC 
GTAAGGAATA AGGAAGAGAT GGCTTGTGCA ATGGTGCACA TTCTTCAGAG CACGGGGAGA 
GCCAAGGACT TCCTAACGGA TCTGGTGATG TCTGAGGTGG ATCGCTGCGG GGAACACGAT 
GTCTTGATCT TCAGGGAGAA CACACTTGCT ACCAAAGCTA TTGAGGAATA CCTGAAGTTG 
GTAGGGCAGA AGTATCTCCA TGATGCGCTA GGGGAGTTTA TCAAGGCTTT GTATGAGTCA 
GATGAAAACT GTGAAGTGGA CCCCAGCAAA TGTTCATCCA GTGAATTAAC AGACCATCAG 
AGCAACCTGA AAATGTGTTG TGAGCTGGCC TTCTGCAAGA TTATCAATTC CTACTGCGTG 
TTCCCTCGTG AGCTGAAGGA GGTGTTTGCA TCTTGGAAGC AGCAGTGCCT GAGCAGGGGC 
AAGCAGGACA TCAGCGAGCG GCTGATCAGT GCCTCCCTGT TCCTCCGCTT CCTGTGCCCC 
GCCATCATGT CCCCCAGCCT CTTCAGCCTC ATGCAGGAGT ATCCTGACGA CCGGACATCG 
CGCACGCTCA CACTCATTGC TAAAGTCATT CAGAATCTCG CCAATTTTGC TAAGTTTGGT 
AACAAGGAAG AGTACATGGC TTTCATGAAC GACTTTCTGG AACATGAGTG GGGGGGAATG 
AAGCGATTTC TCCTGGAGAT CTCTAATCCA GATACCATCT CAAACATGCC TGGATTTGAG 
GGCTATATCG ACCTGGGCAG AGAGCTCTCA GTTTTGCATT CCCTCTTATG GGAGGTTGTG 
TCCCAACTTG ATAAGGGTGA AAATTCCTTC CTACAGGCGA CCGTGGCAAA ACTGGGACCT 
CTTCCTCGTA TTCTTGCCGA CATCACCAAA TCAATGACCA ACCCTACGCC AATACAGCAG 
CAGCTGAGGC GTTTCACTGA GCACAGCTCT AGTCCAAACG TCAGTGGCAG TCTCTCCTCA 
GGGCTTCAGA AGATATTCGA GGACCCCACT GATGGTGACT TGCATAAACT GAAGTCTCCA 
ACCCAGGAAA ACATAGATGG CTATTTCAGG GGCAAGACCT TGCTGTTGGT TCAGCAGGCG 
TCCACCCAGA GCATGACTTA CTCAGATAAG GACGAAAGAG AGAGCATCTT GCCCAACGGA 
CGTAGCATCT CCCTCATGGA CCTGCAGGAT CCCCATACCG CTCACAGCGA CCACGCTTCC 
ATGATGCACG ACGTGCCTTT GCGCTTGGCC GGCAGCCAGC TCTCCATCAC CCAGGTGGCC 
AACATCAAGC AGCTGTGGGA GACCCAGAGC ACGCCCCAAA GCGCTCCCCA GGTGAGGAGG 
CCGCTGCACC CGGCTTTGAA CCAGCAGGGC AGCCTCCAGC CTCTGTCATT CCAGAACCCG 
GTGTACCACC TCAACAACCC ACCTGCGCCG TTGCCCAAGG CCTCCGTGGA CTCCAGCCTG 
GAGAACCTGA GCACCGCCAG CTCCCAGAGC CGAAGCAACA GCGAAGACTT CAAGCTCAGC 
GGGCCCAGCA ACAGCAGCAT GGAGGACTTC ACCAAGCGCA GCACGCAGAG CGAGGACTTC 
TCTAGGCGGC ACACGGTCCC GGACAAGCAC GTCCCCATCG CGTTGCCCCG CCAGAACAGC 
AGCAGCCAGG CACAGATCCG CAAAATGGAC CAGGCCGGGT TAGGGGCCAG GGCCAAAGCG 
CCGCAGTCGC TGCCGCACAG CGCGTCCCTG CGCAGCACGG GCAGCATGTC GGCCGTGTCG 
GGGGCCGTGG TGACTGAGCC CGTCCAGAAC GGCAGCCGCT CCCGGCAGCA GTCGTCCTCC 
TCCAGGGAGA GCCCTGTGCC GAAGGTGAGG GCCATTCAGA GGCAGCAAAC GCAGCAGGTC 
CAGTCTCCTG TGGACTCAGC CACAATGTCA CCAGTGGAAA GGACAGCAGC GTGGGTTCTC 
AATAACGGGC AGTACGAGGA GGTCGAGGAG GACACAGAGC AGAACCCAGA TGAAGCCAAG 
CATGCTGAGA AGTACGAACA AGAGATAGCA AAGCTGAAGG AACGCTTGAA AGTGTCGAGC 
CGCCGGCTGG AGGAGTACGA GCGGCGGCTC CTGGTGCAGG AGCAGCAAAT GCAGAAGCTG 
CTGATGGAGT ACAAGTCCAG GCTGGAGGAC AGCGAGGAGA GACTGCGCAG GCAACAGGAG 
GAGAAGGACA GCCAGATGAA AAGCATTATC AGCAGGCTCA TGGCAGTTGA AGAGGAACTG 
AAGAAAGATC ATGCAGAGAT GCAAGCTGTC ATCGATGCAA AGCAGAAAAT TATTGATGCA 
CAGGTCATTA ATGGGAATGA GATAATTCAA ACAGGAGAAG CGGATCGTGT CCCTGGACTC 
TGCCAACACG CGGCTGATGA GTGCCCTGAC GCAGGTGAAG GAGCGCTACA GCATGCAAGT 
CCGCAATGGC ATCTCCCCCA CCAACCCCAC CAAGCTTTCC ATCACGGAGA ATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_015290516.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015146002.1 Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X11, mRNA.

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CloneID	OGa152369
Clone ID Related Accession (Same CDS sequence)	XM_015290516.2
Accession Version	XM_015290516.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	ras GTPase-activating protein nGAP isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006095.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015290516.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCTCC TTTCTCCCGG CGATGTGGCC GAGTGGCTGG ATAGCAGCAC AGAAGATGAG 
AAAAGCCTTC TGTCGTTTCT CGAGGTCCCG GCAGAGAGAT CTCCTCGCAG ACGGAGCATC 
TCTGGGACCA GCACATCTGA GAAAACCAAC TCCATGGATG CTGCAAATAC CTCTCCTTTC 
AAAGTATCGG GCTTCTTCAG TAAACGTCTG AAAGGGTCCA TCAAGAGGAC AAAGAGTCAG 
TCAAAGCTGG ACAGGAACAC AAGTTTCCGC CTCCCATCCC TCCGCAATGC TGATGACAGG 
TCACGTGGGC TGCCGAAGCT GAAGGAGTCC CGCTCTCATG AGTCCCTGCT GAGCCCCGGC 
AGCGCAGTGG AAGCCCTGGA CCTTGGGGCA GAAGAAAAGG TCTTTGTAAA GCCACTGCAC 
AGCAGCATCC TCGGACAGGA CTTCTGCTTT GAGGTAACCT ACTCCAGCGG CAGTAAATGC 
TTCAGCTGTT CCTCTGCTGC AGAGAGGGAT AAGTGGATGG AAAACCTACG CAGAACAGTG 
CAGCCGAATA AGGACAACTG CCGTCGAGCT GAAAATGTGC TCCGTCTCTG GATCATTGAG 
GCAAAGGACC TGGCCCCCAA GAAGAAATAC TTCTGTGAGC TGTGCCTTGA TGACACTCTC 
TTTGCCCGCA CCACCAGCAA AACGAAAGCA GATAACATTT TCTGGGGGGA GCACTTTGAG 
TTCTACGGCC TCCCAGCTCT TCACAGCATC ACGGTTCACA TCTACAAGGA TGTGGAGAAG 
AAAAAGAAAA AGGACAAAAA CAATTACGTC GGCCTGGTCA ACATTCCCAT GGCCACTGTA 
ACGGGGCGCC AGTTTGTGGA AAAATGGTAC CCAGTCAGCA CACCTACACC CAACAAAGGG 
AAATCAGGAG GCCCTTCCAT TCGCATCAAG TCGCGTTATC AGACCATCAC CATCTTACCC 
ATGGAGCAAT ACAAGGAGTT TGCAGAGTTT GTTACCAGCA ACTACACGAT GCTGTGCTCT 
GTGCTGGAGC CAGTCATCAG CGTAAGGAAT AAGGAAGAGA TGGCTTGTGC AATGGTGCAC 
ATTCTTCAGA GCACGGGGAG AGCCAAGGAC TTCCTAACGG ATCTGGTGAT GTCTGAGGTG 
GATCGCTGCG GGGAACACGA TGTCTTGATC TTCAGGGAGA ACACACTTGC TACCAAAGCT 
ATTGAGGAAT ACCTGAAGTT GGTAGGGCAG AAGTATCTCC ATGATGCGCT AGGGGAGTTT 
ATCAAGGCTT TGTATGAGTC AGATGAAAAC TGTGAAGTGG ACCCCAGCAA ATGTTCATCC 
AGTGAATTAA CAGACCATCA GAGCAACCTG AAAATGTGTT GTGAGCTGGC CTTCTGCAAG 
ATTATCAATT CCTACTGCGT GTTCCCTCGT GAGCTGAAGG AGGTGTTTGC ATCTTGGAAG 
CAGCAGTGCC TGAGCAGGGG CAAGCAGGAC ATCAGCGAGC GGCTGATCAG TGCCTCCCTG 
TTCCTCCGCT TCCTGTGCCC CGCCATCATG TCCCCCAGCC TCTTCAGCCT CATGCAGGAG 
TATCCTGACG ACCGGACATC GCGCACGCTC ACACTCATTG CTAAAGTCAT TCAGAATCTC 
GCCAATTTTG CTAAGTTTGG TAACAAGGAA GAGTACATGG CTTTCATGAA CGACTTTCTG 
GAACATGAGT GGGGGGGAAT GAAGCGATTT CTCCTGGAGA TCTCTAATCC AGATACCATC 
TCAAACATGC CTGGATTTGA GGGCTATATC GACCTGGGCA GAGAGCTCTC AGTTTTGCAT 
TCCCTCTTAT GGGAGGTTGT GTCCCAACTT GATAAGGGTG AAAATTCCTT CCTACAGGCG 
ACCGTGGCAA AACTGGGACC TCTTCCTCGT ATTCTTGCCG ACATCACCAA ATCAATGACC 
AACCCTACGC CAATACAGCA GCAGCTGAGG CGTTTCACTG AGCACAGCTC TAGTCCAAAC 
GTCAGTGGCA GTCTCTCCTC AGGGCTTCAG AAGATATTCG AGGACCCCAC TGATGGTGAC 
TTGCATAAAC TGAAGTCTCC AACCCAGGAA AACATAGATG GCTATTTCAG GGGCAAGACC 
TTGCTGTTGG TTCAGCAGGC GTCCACCCAG AGCATGACTT ACTCAGATAA GGACGAAAGA 
GAGAGCATCT TGCCCAACGG ACGTAGCATC TCCCTCATGG ACCTGCAGGA TCCCCATACC 
GCTCACAGCG ACCACGCTTC CATGATGCAC GACGTGCCTT TGCGCTTGGC CGGCAGCCAG 
CTCTCCATCA CCCAGGTGGC CAACATCAAG CAGCTGTGGG AGACCCAGAG CACGCCCCAA 
AGCGCTCCCC AGGTGAGGAG GCCGCTGCAC CCGGCTTTGA ACCAGCAGGG CAGCCTCCAG 
CCTCTGTCAT TCCAGAACCC GGTGTACCAC CTCAACAACC CACCTGCGCC GTTGCCCAAG 
GCCTCCGTGG ACTCCAGCCT GGAGAACCTG AGCACCGCCA GCTCCCAGAG CCGAAGCAAC 
AGCGAAGACT TCAAGCTCAG CGGGCCCAGC AACAGCAGCA TGGAGGACTT CACCAAGCGC 
AGCACGCAGA GCGAGGACTT CTCTAGGCGG CACACGGTCC CGGACAAGCA CGTCCCCATC 
GCGTTGCCCC GCCAGAACAG CAGCAGCCAG GCACAGATCC GCAAAATGGA CCAGGCCGGG 
TTAGGGGCCA GGGCCAAAGC GCCGCAGTCG CTGCCGCACA GCGCGTCCCT GCGCAGCACG 
GGCAGCATGT CGGCCGTGTC GGGGGCCGTG GTGACTGAGC CCGTCCAGAA CGGCAGCCGC 
TCCCGGCAGC AGTCGTCCTC CTCCAGGGAG AGCCCTGTGC CGAAGGTGAG GGCCATTCAG 
AGGCAGCAAA CGCAGCAGGT CCAGTCTCCT GTGGACTCAG CCACAATGTC ACCAGTGGAA 
AGGACAGCAG CGTGGGTTCT CAATAACGGG CAGTACGAGG AGGTCGAGGA GGACACAGAG 
CAGAACCCAG ATGAAGCCAA GCATGCTGAG AAGTACGAAC AAGAGATAGC AAAGCTGAAG 
GAACGCTTGA AAGTGTCGAG CCGCCGGCTG GAGGAGTACG AGCGGCGGCT CCTGGTGCAG 
GAGCAGCAAA TGCAGAAGCT GCTGATGGAG TACAAGTCCA GGCTGGAGGA CAGCGAGGAG 
AGACTGCGCA GGCAACAGGA GGAGAAGGAC AGCCAGATGA AAAGCATTAT CAGCAGGCTC 
ATGGCAGTTG AAGAGGAACT GAAGAAAGAT CATGCAGAGA TGCAAGCTGT CATCGATGCA 
AAGCAGAAAA TTATTGATGC ACAGGTCATT AATGGGAATG AGATAATTCA AACAGGAGAA 
GCGGATCGTG TCCCTGGACT CTGCCAACAC GCGGCTGATG AGTGCCCTGA CGCAGGTGAA 
GGAGCGCTAC AGCATGCAAG TCCGCAATGG CATCTCCCCC ACCAACCCCA CCAAGCTTTC 
CATCACGGAG AATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015146002.1
CDS	16..3513
Translation	MSLLSPGDVAEWLDSSTEDEKSLLSFLEVPAERSPRRRSISGTSTSEKTNSMDAANTSPFKVSGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNADDRSRGLPKLKESRSHESLLSPGSAVEALDLGAEEKVFVKPLHSSILGQDFCFEVTYSSGSKCFSCSSAAERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYGLPALHSITVHIYKDVEKKKKKDKNNYVGLVNIPMATVTGRQFVEKWYPVSTPTPNKGKSGGPSIRIKSRYQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEEMACAMVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTLATKAIEEYLKLVGQKYLHDALGEFIKALYESDENCEVDPSKCSSSELTDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCLSRGKQDISERLISASLFLRFLCPAIMSPSLFSLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNMPGFEGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVAKLGPLPRILADITKSMTNPTPIQQQLRRFTEHSSSPNVSGSLSSGLQKIFEDPTDGDLHKLKSPTQENIDGYFRGKTLLLVQQASTQSMTYSDKDERESILPNGRSISLMDLQDPHTAHSDHASMMHDVPLRLAGSQLSITQVANIKQLWETQSTPQSAPQVRRPLHPALNQQGSLQPLSFQNPVYHLNNPPAPLPKASVDSSLENLSTASSQSRSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTVPDKHVPIALPRQNSSSQAQIRKMDQAGLGARAKAPQSLPHSASLRSTGSMSAVSGAVVTEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEVEEDTEQNPDEAKHAEKYEQEIAKLKERLKVSSRRLEEYERRLLVQEQQMQKLLMEYKSRLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQVINGNEIIQTGEADRVPGLCQHAADECPDAGEGALQHASPQWHLPHQPHQAFHHGEW

Target ORF information:

RefSeq Version	XM_015290516.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015290516.2

ORF Insert Sequence:

ATGAGTCTCC TTTCTCCCGG CGATGTGGCC GAGTGGCTGG ATAGCAGCAC AGAAGATGAG 
AAAAGCCTTC TGTCGTTTCT CGAGGTCCCG GCAGAGAGAT CTCCTCGCAG ACGGAGCATC 
TCTGGGACCA GCACATCTGA GAAAACCAAC TCCATGGATG CTGCAAATAC CTCTCCTTTC 
AAAGTATCGG GCTTCTTCAG TAAACGTCTG AAAGGGTCCA TCAAGAGGAC AAAGAGTCAG 
TCAAAGCTGG ACAGGAACAC AAGTTTCCGC CTCCCATCCC TCCGCAATGC TGATGACAGG 
TCACGTGGGC TGCCGAAGCT GAAGGAGTCC CGCTCTCATG AGTCCCTGCT GAGCCCCGGC 
AGCGCAGTGG AAGCCCTGGA CCTTGGGGCA GAAGAAAAGG TCTTTGTAAA GCCACTGCAC 
AGCAGCATCC TCGGACAGGA CTTCTGCTTT GAGGTAACCT ACTCCAGCGG CAGTAAATGC 
TTCAGCTGTT CCTCTGCTGC AGAGAGGGAT AAGTGGATGG AAAACCTACG CAGAACAGTG 
CAGCCGAATA AGGACAACTG CCGTCGAGCT GAAAATGTGC TCCGTCTCTG GATCATTGAG 
GCAAAGGACC TGGCCCCCAA GAAGAAATAC TTCTGTGAGC TGTGCCTTGA TGACACTCTC 
TTTGCCCGCA CCACCAGCAA AACGAAAGCA GATAACATTT TCTGGGGGGA GCACTTTGAG 
TTCTACGGCC TCCCAGCTCT TCACAGCATC ACGGTTCACA TCTACAAGGA TGTGGAGAAG 
AAAAAGAAAA AGGACAAAAA CAATTACGTC GGCCTGGTCA ACATTCCCAT GGCCACTGTA 
ACGGGGCGCC AGTTTGTGGA AAAATGGTAC CCAGTCAGCA CACCTACACC CAACAAAGGG 
AAATCAGGAG GCCCTTCCAT TCGCATCAAG TCGCGTTATC AGACCATCAC CATCTTACCC 
ATGGAGCAAT ACAAGGAGTT TGCAGAGTTT GTTACCAGCA ACTACACGAT GCTGTGCTCT 
GTGCTGGAGC CAGTCATCAG CGTAAGGAAT AAGGAAGAGA TGGCTTGTGC AATGGTGCAC 
ATTCTTCAGA GCACGGGGAG AGCCAAGGAC TTCCTAACGG ATCTGGTGAT GTCTGAGGTG 
GATCGCTGCG GGGAACACGA TGTCTTGATC TTCAGGGAGA ACACACTTGC TACCAAAGCT 
ATTGAGGAAT ACCTGAAGTT GGTAGGGCAG AAGTATCTCC ATGATGCGCT AGGGGAGTTT 
ATCAAGGCTT TGTATGAGTC AGATGAAAAC TGTGAAGTGG ACCCCAGCAA ATGTTCATCC 
AGTGAATTAA CAGACCATCA GAGCAACCTG AAAATGTGTT GTGAGCTGGC CTTCTGCAAG 
ATTATCAATT CCTACTGCGT GTTCCCTCGT GAGCTGAAGG AGGTGTTTGC ATCTTGGAAG 
CAGCAGTGCC TGAGCAGGGG CAAGCAGGAC ATCAGCGAGC GGCTGATCAG TGCCTCCCTG 
TTCCTCCGCT TCCTGTGCCC CGCCATCATG TCCCCCAGCC TCTTCAGCCT CATGCAGGAG 
TATCCTGACG ACCGGACATC GCGCACGCTC ACACTCATTG CTAAAGTCAT TCAGAATCTC 
GCCAATTTTG CTAAGTTTGG TAACAAGGAA GAGTACATGG CTTTCATGAA CGACTTTCTG 
GAACATGAGT GGGGGGGAAT GAAGCGATTT CTCCTGGAGA TCTCTAATCC AGATACCATC 
TCAAACATGC CTGGATTTGA GGGCTATATC GACCTGGGCA GAGAGCTCTC AGTTTTGCAT 
TCCCTCTTAT GGGAGGTTGT GTCCCAACTT GATAAGGGTG AAAATTCCTT CCTACAGGCG 
ACCGTGGCAA AACTGGGACC TCTTCCTCGT ATTCTTGCCG ACATCACCAA ATCAATGACC 
AACCCTACGC CAATACAGCA GCAGCTGAGG CGTTTCACTG AGCACAGCTC TAGTCCAAAC 
GTCAGTGGCA GTCTCTCCTC AGGGCTTCAG AAGATATTCG AGGACCCCAC TGATGGTGAC 
TTGCATAAAC TGAAGTCTCC AACCCAGGAA AACATAGATG GCTATTTCAG GGGCAAGACC 
TTGCTGTTGG TTCAGCAGGC GTCCACCCAG AGCATGACTT ACTCAGATAA GGACGAAAGA 
GAGAGCATCT TGCCCAACGG ACGTAGCATC TCCCTCATGG ACCTGCAGGA TCCCCATACC 
GCTCACAGCG ACCACGCTTC CATGATGCAC GACGTGCCTT TGCGCTTGGC CGGCAGCCAG 
CTCTCCATCA CCCAGGTGGC CAACATCAAG CAGCTGTGGG AGACCCAGAG CACGCCCCAA 
AGCGCTCCCC AGGTGAGGAG GCCGCTGCAC CCGGCTTTGA ACCAGCAGGG CAGCCTCCAG 
CCTCTGTCAT TCCAGAACCC GGTGTACCAC CTCAACAACC CACCTGCGCC GTTGCCCAAG 
GCCTCCGTGG ACTCCAGCCT GGAGAACCTG AGCACCGCCA GCTCCCAGAG CCGAAGCAAC 
AGCGAAGACT TCAAGCTCAG CGGGCCCAGC AACAGCAGCA TGGAGGACTT CACCAAGCGC 
AGCACGCAGA GCGAGGACTT CTCTAGGCGG CACACGGTCC CGGACAAGCA CGTCCCCATC 
GCGTTGCCCC GCCAGAACAG CAGCAGCCAG GCACAGATCC GCAAAATGGA CCAGGCCGGG 
TTAGGGGCCA GGGCCAAAGC GCCGCAGTCG CTGCCGCACA GCGCGTCCCT GCGCAGCACG 
GGCAGCATGT CGGCCGTGTC GGGGGCCGTG GTGACTGAGC CCGTCCAGAA CGGCAGCCGC 
TCCCGGCAGC AGTCGTCCTC CTCCAGGGAG AGCCCTGTGC CGAAGGTGAG GGCCATTCAG 
AGGCAGCAAA CGCAGCAGGT CCAGTCTCCT GTGGACTCAG CCACAATGTC ACCAGTGGAA 
AGGACAGCAG CGTGGGTTCT CAATAACGGG CAGTACGAGG AGGTCGAGGA GGACACAGAG 
CAGAACCCAG ATGAAGCCAA GCATGCTGAG AAGTACGAAC AAGAGATAGC AAAGCTGAAG 
GAACGCTTGA AAGTGTCGAG CCGCCGGCTG GAGGAGTACG AGCGGCGGCT CCTGGTGCAG 
GAGCAGCAAA TGCAGAAGCT GCTGATGGAG TACAAGTCCA GGCTGGAGGA CAGCGAGGAG 
AGACTGCGCA GGCAACAGGA GGAGAAGGAC AGCCAGATGA AAAGCATTAT CAGCAGGCTC 
ATGGCAGTTG AAGAGGAACT GAAGAAAGAT CATGCAGAGA TGCAAGCTGT CATCGATGCA 
AAGCAGAAAA TTATTGATGC ACAGGTCATT AATGGGAATG AGATAATTCA AACAGGAGAA 
GCGGATCGTG TCCCTGGACT CTGCCAACAC GCGGCTGATG AGTGCCCTGA CGCAGGTGAA 
GGAGCGCTAC AGCATGCAAG TCCGCAATGG CATCTCCCCC ACCAACCCCA CCAAGCTTTC 
CATCACGGAG AATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_004943254.3 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004943311.2 Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X12, mRNA.

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CloneID	OGa152370
Clone ID Related Accession (Same CDS sequence)	XM_004943254.3
Accession Version	XM_004943254.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	ras GTPase-activating protein nGAP isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006095.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004943254.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAGCA CTTGTGACAA AGAGGTCCCG GCAGAGAGAT CTCCTCGCAG ACGGAGCATC 
TCTGGGACCA GCACATCTGA GAAAACCAAC TCCATGGATG CTGCAAATAC CTCTCCTTTC 
AAAGTATCGG GCTTCTTCAG TAAACGTCTG AAAGGGTCCA TCAAGAGGAC AAAGAGTCAG 
TCAAAGCTGG ACAGGAACAC AAGTTTCCGC CTCCCATCCC TCCGCAATGC TGATGACAGG 
TCACGTGGGC TGCCGAAGCT GAAGGAGTCC CGCTCTCATG AGTCCCTGCT GAGCCCCGGC 
AGCGCAGTGG AAGCCCTGGA CCTTGGGGCA GAAGAAAAGG TCTTTGTAAA GCCACTGCAC 
AGCAGCATCC TCGGACAGGA CTTCTGCTTT GAGGTAACCT ACTCCAGCGG CAGTAAATGC 
TTCAGCTGTT CCTCTGCTGC AGAGAGGGAT AAGTGGATGG AAAACCTACG CAGAACAGTG 
CAGCCGAATA AGGACAACTG CCGTCGAGCT GAAAATGTGC TCCGTCTCTG GATCATTGAG 
GCAAAGGACC TGGCCCCCAA GAAGAAATAC TTCTGTGAGC TGTGCCTTGA TGACACTCTC 
TTTGCCCGCA CCACCAGCAA AACGAAAGCA GATAACATTT TCTGGGGGGA GCACTTTGAG 
TTCTACGGCC TCCCAGCTCT TCACAGCATC ACGGTTCACA TCTACAAGGA TGTGGAGAAG 
AAAAAGAAAA AGGACAAAAA CAATTACGTC GGCCTGGTCA ACATTCCCAT GGCCACTGTA 
ACGGGGCGCC AGTTTGTGGA AAAATGGTAC CCAGTCAGCA CACCTACACC CAACAAAGGG 
AAATCAGGAG GCCCTTCCAT TCGCATCAAG TCGCGTTATC AGACCATCAC CATCTTACCC 
ATGGAGCAAT ACAAGGAGTT TGCAGAGTTT GTTACCAGCA ACTACACGAT GCTGTGCTCT 
GTGCTGGAGC CAGTCATCAG CGTAAGGAAT AAGGAAGAGA TGGCTTGTGC AATGGTGCAC 
ATTCTTCAGA GCACGGGGAG AGCCAAGGAC TTCCTAACGG ATCTGGTGAT GTCTGAGGTG 
GATCGCTGCG GGGAACACGA TGTCTTGATC TTCAGGGAGA ACACACTTGC TACCAAAGCT 
ATTGAGGAAT ACCTGAAGTT GGTAGGGCAG AAGTATCTCC ATGATGCGCT AGGGGAGTTT 
ATCAAGGCTT TGTATGAGTC AGATGAAAAC TGTGAAGTGG ACCCCAGCAA ATGTTCATCC 
AGTGAATTAA CAGACCATCA GAGCAACCTG AAAATGTGTT GTGAGCTGGC CTTCTGCAAG 
ATTATCAATT CCTACTGCGT GTTCCCTCGT GAGCTGAAGG AGGTGTTTGC ATCTTGGAAG 
CAGCAGTGCC TGAGCAGGGG CAAGCAGGAC ATCAGCGAGC GGCTGATCAG TGCCTCCCTG 
TTCCTCCGCT TCCTGTGCCC CGCCATCATG TCCCCCAGCC TCTTCAGCCT CATGCAGGAG 
TATCCTGACG ACCGGACATC GCGCACGCTC ACACTCATTG CTAAAGTCAT TCAGAATCTC 
GCCAATTTTG CTAAGTTTGG TAACAAGGAA GAGTACATGG CTTTCATGAA CGACTTTCTG 
GAACATGAGT GGGGGGGAAT GAAGCGATTT CTCCTGGAGA TCTCTAATCC AGATACCATC 
TCAAACATGC CTGGATTTGA GGGCTATATC GACCTGGGCA GAGAGCTCTC AGTTTTGCAT 
TCCCTCTTAT GGGAGGTTGT GTCCCAACTT GATAAGGGTG AAAATTCCTT CCTACAGGCG 
ACCGTGGCAA AACTGGGACC TCTTCCTCGT ATTCTTGCCG ACATCACCAA ATCAATGACC 
AACCCTACGC CAATACAGCA GCAGCTGAGG CGTTTCACTG AGCACAGCTC TAGTCCAAAC 
GTCAGTGGCA GTCTCTCCTC AGGGCTTCAG AAGATATTCG AGGACCCCAC TGATGGTGAC 
TTGCATAAAC TGAAGTCTCC AACCCAGGAA AACATAGATG GCTATTTCAG GGGCAAGACC 
TTGCTGTTGG TTCAGCAGGC GTCCACCCAG AGCATGACTT ACTCAGATAA GGACGAAAGA 
GAGAGCATCT TGCCCAACGG ACGTAGCATC TCCCTCATGG ACCTGCAGGA TCCCCATACC 
GCTCACAGCG ACCACGCTTC CATGATGCAC GACGTGCCTT TGCGCTTGGC CGGCAGCCAG 
CTCTCCATCA CCCAGGTGGC CAACATCAAG CAGCTGTGGG AGACCCAGAG CACGCCCCAA 
AGCGCTCCCC AGGTGAGGAG GCCGCTGCAC CCGGCTTTGA ACCAGCAGGG CAGCCTCCAG 
CCTCTGTCAT TCCAGAACCC GGTGTACCAC CTCAACAACC CACCTGCGCC GTTGCCCAAG 
GCCTCCGTGG ACTCCAGCCT GGAGAACCTG AGCACCGCCA GCTCCCAGAG CCGAAGCAAC 
AGCGAAGACT TCAAGCTCAG CGGGCCCAGC AACAGCAGCA TGGAGGACTT CACCAAGCGC 
AGCACGCAGA GCGAGGACTT CTCTAGGCGG CACACGGTCC CGGACAAGCA CGTCCCCATC 
GCGTTGCCCC GCCAGAACAG CAGCAGCCAG GCACAGATCC GCAAAATGGA CCAGGCCGGG 
TTAGGGGCCA GGGCCAAAGC GCCGCAGTCG CTGCCGCACA GCGCGTCCCT GCGCAGCACG 
GGCAGCATGT CGGCCGTGTC GGGGGCCGTG GTGACTGAGC CCGTCCAGAA CGGCAGCCGC 
TCCCGGCAGC AGTCGTCCTC CTCCAGGGAG AGCCCTGTGC CGAAGGTGAG GGCCATTCAG 
AGGCAGCAAA CGCAGCAGGT CCAGTCTCCT GTGGACTCAG CCACAATGTC ACCAGTGGAA 
AGGACAGCAG CGTGGGTTCT CAATAACGGG CAGTACGAGG AGGTCGAGGA GGACACAGAG 
CAGAACCCAG ATGAAGCCAA GCATGCTGAG AAGTACGAAC AAGAGATAGC AAAGCTGAAG 
GAACGCTTGA AAGTGTCGAG CCGCCGGCTG GAGGAGTACG AGCGGCGGCT CCTGGTGCAG 
GAGCAGCAAA TGCAGAAGCT GCTGATGGAG TACAAGTCCA GGCTGGAGGA CAGCGAGGAG 
AGACTGCGCA GGCAACAGGA GGAGAAGGAC AGCCAGATGA AAAGCATTAT CAGCAGGCTC 
ATGGCAGTTG AAGAGGAACT GAAGAAAGAT CATGCAGAGA TGCAAGCTGT CATCGATGCA 
AAGCAGAAAA TTATTGATGC ACAGGTCATT AATGGGAATG AGATAATTCA AACAGGAGAA 
GCGGATCGTG TCCCTGGACT CTGCCAACAC GCGGCTGATG AGTGCCCTGA CGCAGGTGAA 
GGAGCGCTAC AGCATGCAAG TCCGCAATGG CATCTCCCCC ACCAACCCCA CCAAGCTTTC 
CATCACGGAG AATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004943311.2
CDS	757..4194
Translation	MGSTCDKEVPAERSPRRRSISGTSTSEKTNSMDAANTSPFKVSGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNADDRSRGLPKLKESRSHESLLSPGSAVEALDLGAEEKVFVKPLHSSILGQDFCFEVTYSSGSKCFSCSSAAERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYGLPALHSITVHIYKDVEKKKKKDKNNYVGLVNIPMATVTGRQFVEKWYPVSTPTPNKGKSGGPSIRIKSRYQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEEMACAMVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTLATKAIEEYLKLVGQKYLHDALGEFIKALYESDENCEVDPSKCSSSELTDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCLSRGKQDISERLISASLFLRFLCPAIMSPSLFSLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNMPGFEGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVAKLGPLPRILADITKSMTNPTPIQQQLRRFTEHSSSPNVSGSLSSGLQKIFEDPTDGDLHKLKSPTQENIDGYFRGKTLLLVQQASTQSMTYSDKDERESILPNGRSISLMDLQDPHTAHSDHASMMHDVPLRLAGSQLSITQVANIKQLWETQSTPQSAPQVRRPLHPALNQQGSLQPLSFQNPVYHLNNPPAPLPKASVDSSLENLSTASSQSRSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTVPDKHVPIALPRQNSSSQAQIRKMDQAGLGARAKAPQSLPHSASLRSTGSMSAVSGAVVTEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEVEEDTEQNPDEAKHAEKYEQEIAKLKERLKVSSRRLEEYERRLLVQEQQMQKLLMEYKSRLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQVINGNEIIQTGEADRVPGLCQHAADECPDAGEGALQHASPQWHLPHQPHQAFHHGEW

Target ORF information:

RefSeq Version	XM_004943254.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004943254.3

ORF Insert Sequence:

ATGGGCAGCA CTTGTGACAA AGAGGTCCCG GCAGAGAGAT CTCCTCGCAG ACGGAGCATC 
TCTGGGACCA GCACATCTGA GAAAACCAAC TCCATGGATG CTGCAAATAC CTCTCCTTTC 
AAAGTATCGG GCTTCTTCAG TAAACGTCTG AAAGGGTCCA TCAAGAGGAC AAAGAGTCAG 
TCAAAGCTGG ACAGGAACAC AAGTTTCCGC CTCCCATCCC TCCGCAATGC TGATGACAGG 
TCACGTGGGC TGCCGAAGCT GAAGGAGTCC CGCTCTCATG AGTCCCTGCT GAGCCCCGGC 
AGCGCAGTGG AAGCCCTGGA CCTTGGGGCA GAAGAAAAGG TCTTTGTAAA GCCACTGCAC 
AGCAGCATCC TCGGACAGGA CTTCTGCTTT GAGGTAACCT ACTCCAGCGG CAGTAAATGC 
TTCAGCTGTT CCTCTGCTGC AGAGAGGGAT AAGTGGATGG AAAACCTACG CAGAACAGTG 
CAGCCGAATA AGGACAACTG CCGTCGAGCT GAAAATGTGC TCCGTCTCTG GATCATTGAG 
GCAAAGGACC TGGCCCCCAA GAAGAAATAC TTCTGTGAGC TGTGCCTTGA TGACACTCTC 
TTTGCCCGCA CCACCAGCAA AACGAAAGCA GATAACATTT TCTGGGGGGA GCACTTTGAG 
TTCTACGGCC TCCCAGCTCT TCACAGCATC ACGGTTCACA TCTACAAGGA TGTGGAGAAG 
AAAAAGAAAA AGGACAAAAA CAATTACGTC GGCCTGGTCA ACATTCCCAT GGCCACTGTA 
ACGGGGCGCC AGTTTGTGGA AAAATGGTAC CCAGTCAGCA CACCTACACC CAACAAAGGG 
AAATCAGGAG GCCCTTCCAT TCGCATCAAG TCGCGTTATC AGACCATCAC CATCTTACCC 
ATGGAGCAAT ACAAGGAGTT TGCAGAGTTT GTTACCAGCA ACTACACGAT GCTGTGCTCT 
GTGCTGGAGC CAGTCATCAG CGTAAGGAAT AAGGAAGAGA TGGCTTGTGC AATGGTGCAC 
ATTCTTCAGA GCACGGGGAG AGCCAAGGAC TTCCTAACGG ATCTGGTGAT GTCTGAGGTG 
GATCGCTGCG GGGAACACGA TGTCTTGATC TTCAGGGAGA ACACACTTGC TACCAAAGCT 
ATTGAGGAAT ACCTGAAGTT GGTAGGGCAG AAGTATCTCC ATGATGCGCT AGGGGAGTTT 
ATCAAGGCTT TGTATGAGTC AGATGAAAAC TGTGAAGTGG ACCCCAGCAA ATGTTCATCC 
AGTGAATTAA CAGACCATCA GAGCAACCTG AAAATGTGTT GTGAGCTGGC CTTCTGCAAG 
ATTATCAATT CCTACTGCGT GTTCCCTCGT GAGCTGAAGG AGGTGTTTGC ATCTTGGAAG 
CAGCAGTGCC TGAGCAGGGG CAAGCAGGAC ATCAGCGAGC GGCTGATCAG TGCCTCCCTG 
TTCCTCCGCT TCCTGTGCCC CGCCATCATG TCCCCCAGCC TCTTCAGCCT CATGCAGGAG 
TATCCTGACG ACCGGACATC GCGCACGCTC ACACTCATTG CTAAAGTCAT TCAGAATCTC 
GCCAATTTTG CTAAGTTTGG TAACAAGGAA GAGTACATGG CTTTCATGAA CGACTTTCTG 
GAACATGAGT GGGGGGGAAT GAAGCGATTT CTCCTGGAGA TCTCTAATCC AGATACCATC 
TCAAACATGC CTGGATTTGA GGGCTATATC GACCTGGGCA GAGAGCTCTC AGTTTTGCAT 
TCCCTCTTAT GGGAGGTTGT GTCCCAACTT GATAAGGGTG AAAATTCCTT CCTACAGGCG 
ACCGTGGCAA AACTGGGACC TCTTCCTCGT ATTCTTGCCG ACATCACCAA ATCAATGACC 
AACCCTACGC CAATACAGCA GCAGCTGAGG CGTTTCACTG AGCACAGCTC TAGTCCAAAC 
GTCAGTGGCA GTCTCTCCTC AGGGCTTCAG AAGATATTCG AGGACCCCAC TGATGGTGAC 
TTGCATAAAC TGAAGTCTCC AACCCAGGAA AACATAGATG GCTATTTCAG GGGCAAGACC 
TTGCTGTTGG TTCAGCAGGC GTCCACCCAG AGCATGACTT ACTCAGATAA GGACGAAAGA 
GAGAGCATCT TGCCCAACGG ACGTAGCATC TCCCTCATGG ACCTGCAGGA TCCCCATACC 
GCTCACAGCG ACCACGCTTC CATGATGCAC GACGTGCCTT TGCGCTTGGC CGGCAGCCAG 
CTCTCCATCA CCCAGGTGGC CAACATCAAG CAGCTGTGGG AGACCCAGAG CACGCCCCAA 
AGCGCTCCCC AGGTGAGGAG GCCGCTGCAC CCGGCTTTGA ACCAGCAGGG CAGCCTCCAG 
CCTCTGTCAT TCCAGAACCC GGTGTACCAC CTCAACAACC CACCTGCGCC GTTGCCCAAG 
GCCTCCGTGG ACTCCAGCCT GGAGAACCTG AGCACCGCCA GCTCCCAGAG CCGAAGCAAC 
AGCGAAGACT TCAAGCTCAG CGGGCCCAGC AACAGCAGCA TGGAGGACTT CACCAAGCGC 
AGCACGCAGA GCGAGGACTT CTCTAGGCGG CACACGGTCC CGGACAAGCA CGTCCCCATC 
GCGTTGCCCC GCCAGAACAG CAGCAGCCAG GCACAGATCC GCAAAATGGA CCAGGCCGGG 
TTAGGGGCCA GGGCCAAAGC GCCGCAGTCG CTGCCGCACA GCGCGTCCCT GCGCAGCACG 
GGCAGCATGT CGGCCGTGTC GGGGGCCGTG GTGACTGAGC CCGTCCAGAA CGGCAGCCGC 
TCCCGGCAGC AGTCGTCCTC CTCCAGGGAG AGCCCTGTGC CGAAGGTGAG GGCCATTCAG 
AGGCAGCAAA CGCAGCAGGT CCAGTCTCCT GTGGACTCAG CCACAATGTC ACCAGTGGAA 
AGGACAGCAG CGTGGGTTCT CAATAACGGG CAGTACGAGG AGGTCGAGGA GGACACAGAG 
CAGAACCCAG ATGAAGCCAA GCATGCTGAG AAGTACGAAC AAGAGATAGC AAAGCTGAAG 
GAACGCTTGA AAGTGTCGAG CCGCCGGCTG GAGGAGTACG AGCGGCGGCT CCTGGTGCAG 
GAGCAGCAAA TGCAGAAGCT GCTGATGGAG TACAAGTCCA GGCTGGAGGA CAGCGAGGAG 
AGACTGCGCA GGCAACAGGA GGAGAAGGAC AGCCAGATGA AAAGCATTAT CAGCAGGCTC 
ATGGCAGTTG AAGAGGAACT GAAGAAAGAT CATGCAGAGA TGCAAGCTGT CATCGATGCA 
AAGCAGAAAA TTATTGATGC ACAGGTCATT AATGGGAATG AGATAATTCA AACAGGAGAA 
GCGGATCGTG TCCCTGGACT CTGCCAACAC GCGGCTGATG AGTGCCCTGA CGCAGGTGAA 
GGAGCGCTAC AGCATGCAAG TCCGCAATGG CATCTCCCCC ACCAACCCCA CCAAGCTTTC 
CATCACGGAG AATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_015290519.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015146005.1 Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X13, mRNA.

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CloneID	OGa152371
Clone ID Related Accession (Same CDS sequence)	XM_015290519.2
Accession Version	XM_015290519.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3429bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	ras GTPase-activating protein nGAP isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006095.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015290519.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCTCG CAGAGGTCCC GGCAGAGAGA TCTCCTCGCA GACGGAGCAT CTCTGGGACC 
AGCACATCTG AGAAAACCAA CTCCATGGAT GCTGCAAATA CCTCTCCTTT CAAAGTATCG 
GGCTTCTTCA GTAAACGTCT GAAAGGGTCC ATCAAGAGGA CAAAGAGTCA GTCAAAGCTG 
GACAGGAACA CAAGTTTCCG CCTCCCATCC CTCCGCAATG CTGATGACAG GTCACGTGGG 
CTGCCGAAGC TGAAGGAGTC CCGCTCTCAT GAGTCCCTGC TGAGCCCCGG CAGCGCAGTG 
GAAGCCCTGG ACCTTGGGGC AGAAGAAAAG GTCTTTGTAA AGCCACTGCA CAGCAGCATC 
CTCGGACAGG ACTTCTGCTT TGAGGTAACC TACTCCAGCG GCAGTAAATG CTTCAGCTGT 
TCCTCTGCTG CAGAGAGGGA TAAGTGGATG GAAAACCTAC GCAGAACAGT GCAGCCGAAT 
AAGGACAACT GCCGTCGAGC TGAAAATGTG CTCCGTCTCT GGATCATTGA GGCAAAGGAC 
CTGGCCCCCA AGAAGAAATA CTTCTGTGAG CTGTGCCTTG ATGACACTCT CTTTGCCCGC 
ACCACCAGCA AAACGAAAGC AGATAACATT TTCTGGGGGG AGCACTTTGA GTTCTACGGC 
CTCCCAGCTC TTCACAGCAT CACGGTTCAC ATCTACAAGG ATGTGGAGAA GAAAAAGAAA 
AAGGACAAAA ACAATTACGT CGGCCTGGTC AACATTCCCA TGGCCACTGT AACGGGGCGC 
CAGTTTGTGG AAAAATGGTA CCCAGTCAGC ACACCTACAC CCAACAAAGG GAAATCAGGA 
GGCCCTTCCA TTCGCATCAA GTCGCGTTAT CAGACCATCA CCATCTTACC CATGGAGCAA 
TACAAGGAGT TTGCAGAGTT TGTTACCAGC AACTACACGA TGCTGTGCTC TGTGCTGGAG 
CCAGTCATCA GCGTAAGGAA TAAGGAAGAG ATGGCTTGTG CAATGGTGCA CATTCTTCAG 
AGCACGGGGA GAGCCAAGGA CTTCCTAACG GATCTGGTGA TGTCTGAGGT GGATCGCTGC 
GGGGAACACG ATGTCTTGAT CTTCAGGGAG AACACACTTG CTACCAAAGC TATTGAGGAA 
TACCTGAAGT TGGTAGGGCA GAAGTATCTC CATGATGCGC TAGGGGAGTT TATCAAGGCT 
TTGTATGAGT CAGATGAAAA CTGTGAAGTG GACCCCAGCA AATGTTCATC CAGTGAATTA 
ACAGACCATC AGAGCAACCT GAAAATGTGT TGTGAGCTGG CCTTCTGCAA GATTATCAAT 
TCCTACTGCG TGTTCCCTCG TGAGCTGAAG GAGGTGTTTG CATCTTGGAA GCAGCAGTGC 
CTGAGCAGGG GCAAGCAGGA CATCAGCGAG CGGCTGATCA GTGCCTCCCT GTTCCTCCGC 
TTCCTGTGCC CCGCCATCAT GTCCCCCAGC CTCTTCAGCC TCATGCAGGA GTATCCTGAC 
GACCGGACAT CGCGCACGCT CACACTCATT GCTAAAGTCA TTCAGAATCT CGCCAATTTT 
GCTAAGTTTG GTAACAAGGA AGAGTACATG GCTTTCATGA ACGACTTTCT GGAACATGAG 
TGGGGGGGAA TGAAGCGATT TCTCCTGGAG ATCTCTAATC CAGATACCAT CTCAAACATG 
CCTGGATTTG AGGGCTATAT CGACCTGGGC AGAGAGCTCT CAGTTTTGCA TTCCCTCTTA 
TGGGAGGTTG TGTCCCAACT TGATAAGGGT GAAAATTCCT TCCTACAGGC GACCGTGGCA 
AAACTGGGAC CTCTTCCTCG TATTCTTGCC GACATCACCA AATCAATGAC CAACCCTACG 
CCAATACAGC AGCAGCTGAG GCGTTTCACT GAGCACAGCT CTAGTCCAAA CGTCAGTGGC 
AGTCTCTCCT CAGGGCTTCA GAAGATATTC GAGGACCCCA CTGATGGTGA CTTGCATAAA 
CTGAAGTCTC CAACCCAGGA AAACATAGAT GGCTATTTCA GGGGCAAGAC CTTGCTGTTG 
GTTCAGCAGG CGTCCACCCA GAGCATGACT TACTCAGATA AGGACGAAAG AGAGAGCATC 
TTGCCCAACG GACGTAGCAT CTCCCTCATG GACCTGCAGG ATCCCCATAC CGCTCACAGC 
GACCACGCTT CCATGATGCA CGACGTGCCT TTGCGCTTGG CCGGCAGCCA GCTCTCCATC 
ACCCAGGTGG CCAACATCAA GCAGCTGTGG GAGACCCAGA GCACGCCCCA AAGCGCTCCC 
CAGGTGAGGA GGCCGCTGCA CCCGGCTTTG AACCAGCAGG GCAGCCTCCA GCCTCTGTCA 
TTCCAGAACC CGGTGTACCA CCTCAACAAC CCACCTGCGC CGTTGCCCAA GGCCTCCGTG 
GACTCCAGCC TGGAGAACCT GAGCACCGCC AGCTCCCAGA GCCGAAGCAA CAGCGAAGAC 
TTCAAGCTCA GCGGGCCCAG CAACAGCAGC ATGGAGGACT TCACCAAGCG CAGCACGCAG 
AGCGAGGACT TCTCTAGGCG GCACACGGTC CCGGACAAGC ACGTCCCCAT CGCGTTGCCC 
CGCCAGAACA GCAGCAGCCA GGCACAGATC CGCAAAATGG ACCAGGCCGG GTTAGGGGCC 
AGGGCCAAAG CGCCGCAGTC GCTGCCGCAC AGCGCGTCCC TGCGCAGCAC GGGCAGCATG 
TCGGCCGTGT CGGGGGCCGT GGTGACTGAG CCCGTCCAGA ACGGCAGCCG CTCCCGGCAG 
CAGTCGTCCT CCTCCAGGGA GAGCCCTGTG CCGAAGGTGA GGGCCATTCA GAGGCAGCAA 
ACGCAGCAGG TCCAGTCTCC TGTGGACTCA GCCACAATGT CACCAGTGGA AAGGACAGCA 
GCGTGGGTTC TCAATAACGG GCAGTACGAG GAGGTCGAGG AGGACACAGA GCAGAACCCA 
GATGAAGCCA AGCATGCTGA GAAGTACGAA CAAGAGATAG CAAAGCTGAA GGAACGCTTG 
AAAGTGTCGA GCCGCCGGCT GGAGGAGTAC GAGCGGCGGC TCCTGGTGCA GGAGCAGCAA 
ATGCAGAAGC TGCTGATGGA GTACAAGTCC AGGCTGGAGG ACAGCGAGGA GAGACTGCGC 
AGGCAACAGG AGGAGAAGGA CAGCCAGATG AAAAGCATTA TCAGCAGGCT CATGGCAGTT 
GAAGAGGAAC TGAAGAAAGA TCATGCAGAG ATGCAAGCTG TCATCGATGC AAAGCAGAAA 
ATTATTGATG CACAGGTCAT TAATGGGAAT GAGATAATTC AAACAGGAGA AGCGGATCGT 
GTCCCTGGAC TCTGCCAACA CGCGGCTGAT GAGTGCCCTG ACGCAGGTGA AGGAGCGCTA 
CAGCATGCAA GTCCGCAATG GCATCTCCCC CACCAACCCC ACCAAGCTTT CCATCACGGA 
GAATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015146005.1
CDS	238..3666
Translation	MQLAEVPAERSPRRRSISGTSTSEKTNSMDAANTSPFKVSGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNADDRSRGLPKLKESRSHESLLSPGSAVEALDLGAEEKVFVKPLHSSILGQDFCFEVTYSSGSKCFSCSSAAERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYGLPALHSITVHIYKDVEKKKKKDKNNYVGLVNIPMATVTGRQFVEKWYPVSTPTPNKGKSGGPSIRIKSRYQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEEMACAMVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTLATKAIEEYLKLVGQKYLHDALGEFIKALYESDENCEVDPSKCSSSELTDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCLSRGKQDISERLISASLFLRFLCPAIMSPSLFSLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNMPGFEGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVAKLGPLPRILADITKSMTNPTPIQQQLRRFTEHSSSPNVSGSLSSGLQKIFEDPTDGDLHKLKSPTQENIDGYFRGKTLLLVQQASTQSMTYSDKDERESILPNGRSISLMDLQDPHTAHSDHASMMHDVPLRLAGSQLSITQVANIKQLWETQSTPQSAPQVRRPLHPALNQQGSLQPLSFQNPVYHLNNPPAPLPKASVDSSLENLSTASSQSRSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTVPDKHVPIALPRQNSSSQAQIRKMDQAGLGARAKAPQSLPHSASLRSTGSMSAVSGAVVTEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEVEEDTEQNPDEAKHAEKYEQEIAKLKERLKVSSRRLEEYERRLLVQEQQMQKLLMEYKSRLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQVINGNEIIQTGEADRVPGLCQHAADECPDAGEGALQHASPQWHLPHQPHQAFHHGEW

Target ORF information:

RefSeq Version	XM_015290519.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015290519.2

ORF Insert Sequence:

ATGCAGCTCG CAGAGGTCCC GGCAGAGAGA TCTCCTCGCA GACGGAGCAT CTCTGGGACC 
AGCACATCTG AGAAAACCAA CTCCATGGAT GCTGCAAATA CCTCTCCTTT CAAAGTATCG 
GGCTTCTTCA GTAAACGTCT GAAAGGGTCC ATCAAGAGGA CAAAGAGTCA GTCAAAGCTG 
GACAGGAACA CAAGTTTCCG CCTCCCATCC CTCCGCAATG CTGATGACAG GTCACGTGGG 
CTGCCGAAGC TGAAGGAGTC CCGCTCTCAT GAGTCCCTGC TGAGCCCCGG CAGCGCAGTG 
GAAGCCCTGG ACCTTGGGGC AGAAGAAAAG GTCTTTGTAA AGCCACTGCA CAGCAGCATC 
CTCGGACAGG ACTTCTGCTT TGAGGTAACC TACTCCAGCG GCAGTAAATG CTTCAGCTGT 
TCCTCTGCTG CAGAGAGGGA TAAGTGGATG GAAAACCTAC GCAGAACAGT GCAGCCGAAT 
AAGGACAACT GCCGTCGAGC TGAAAATGTG CTCCGTCTCT GGATCATTGA GGCAAAGGAC 
CTGGCCCCCA AGAAGAAATA CTTCTGTGAG CTGTGCCTTG ATGACACTCT CTTTGCCCGC 
ACCACCAGCA AAACGAAAGC AGATAACATT TTCTGGGGGG AGCACTTTGA GTTCTACGGC 
CTCCCAGCTC TTCACAGCAT CACGGTTCAC ATCTACAAGG ATGTGGAGAA GAAAAAGAAA 
AAGGACAAAA ACAATTACGT CGGCCTGGTC AACATTCCCA TGGCCACTGT AACGGGGCGC 
CAGTTTGTGG AAAAATGGTA CCCAGTCAGC ACACCTACAC CCAACAAAGG GAAATCAGGA 
GGCCCTTCCA TTCGCATCAA GTCGCGTTAT CAGACCATCA CCATCTTACC CATGGAGCAA 
TACAAGGAGT TTGCAGAGTT TGTTACCAGC AACTACACGA TGCTGTGCTC TGTGCTGGAG 
CCAGTCATCA GCGTAAGGAA TAAGGAAGAG ATGGCTTGTG CAATGGTGCA CATTCTTCAG 
AGCACGGGGA GAGCCAAGGA CTTCCTAACG GATCTGGTGA TGTCTGAGGT GGATCGCTGC 
GGGGAACACG ATGTCTTGAT CTTCAGGGAG AACACACTTG CTACCAAAGC TATTGAGGAA 
TACCTGAAGT TGGTAGGGCA GAAGTATCTC CATGATGCGC TAGGGGAGTT TATCAAGGCT 
TTGTATGAGT CAGATGAAAA CTGTGAAGTG GACCCCAGCA AATGTTCATC CAGTGAATTA 
ACAGACCATC AGAGCAACCT GAAAATGTGT TGTGAGCTGG CCTTCTGCAA GATTATCAAT 
TCCTACTGCG TGTTCCCTCG TGAGCTGAAG GAGGTGTTTG CATCTTGGAA GCAGCAGTGC 
CTGAGCAGGG GCAAGCAGGA CATCAGCGAG CGGCTGATCA GTGCCTCCCT GTTCCTCCGC 
TTCCTGTGCC CCGCCATCAT GTCCCCCAGC CTCTTCAGCC TCATGCAGGA GTATCCTGAC 
GACCGGACAT CGCGCACGCT CACACTCATT GCTAAAGTCA TTCAGAATCT CGCCAATTTT 
GCTAAGTTTG GTAACAAGGA AGAGTACATG GCTTTCATGA ACGACTTTCT GGAACATGAG 
TGGGGGGGAA TGAAGCGATT TCTCCTGGAG ATCTCTAATC CAGATACCAT CTCAAACATG 
CCTGGATTTG AGGGCTATAT CGACCTGGGC AGAGAGCTCT CAGTTTTGCA TTCCCTCTTA 
TGGGAGGTTG TGTCCCAACT TGATAAGGGT GAAAATTCCT TCCTACAGGC GACCGTGGCA 
AAACTGGGAC CTCTTCCTCG TATTCTTGCC GACATCACCA AATCAATGAC CAACCCTACG 
CCAATACAGC AGCAGCTGAG GCGTTTCACT GAGCACAGCT CTAGTCCAAA CGTCAGTGGC 
AGTCTCTCCT CAGGGCTTCA GAAGATATTC GAGGACCCCA CTGATGGTGA CTTGCATAAA 
CTGAAGTCTC CAACCCAGGA AAACATAGAT GGCTATTTCA GGGGCAAGAC CTTGCTGTTG 
GTTCAGCAGG CGTCCACCCA GAGCATGACT TACTCAGATA AGGACGAAAG AGAGAGCATC 
TTGCCCAACG GACGTAGCAT CTCCCTCATG GACCTGCAGG ATCCCCATAC CGCTCACAGC 
GACCACGCTT CCATGATGCA CGACGTGCCT TTGCGCTTGG CCGGCAGCCA GCTCTCCATC 
ACCCAGGTGG CCAACATCAA GCAGCTGTGG GAGACCCAGA GCACGCCCCA AAGCGCTCCC 
CAGGTGAGGA GGCCGCTGCA CCCGGCTTTG AACCAGCAGG GCAGCCTCCA GCCTCTGTCA 
TTCCAGAACC CGGTGTACCA CCTCAACAAC CCACCTGCGC CGTTGCCCAA GGCCTCCGTG 
GACTCCAGCC TGGAGAACCT GAGCACCGCC AGCTCCCAGA GCCGAAGCAA CAGCGAAGAC 
TTCAAGCTCA GCGGGCCCAG CAACAGCAGC ATGGAGGACT TCACCAAGCG CAGCACGCAG 
AGCGAGGACT TCTCTAGGCG GCACACGGTC CCGGACAAGC ACGTCCCCAT CGCGTTGCCC 
CGCCAGAACA GCAGCAGCCA GGCACAGATC CGCAAAATGG ACCAGGCCGG GTTAGGGGCC 
AGGGCCAAAG CGCCGCAGTC GCTGCCGCAC AGCGCGTCCC TGCGCAGCAC GGGCAGCATG 
TCGGCCGTGT CGGGGGCCGT GGTGACTGAG CCCGTCCAGA ACGGCAGCCG CTCCCGGCAG 
CAGTCGTCCT CCTCCAGGGA GAGCCCTGTG CCGAAGGTGA GGGCCATTCA GAGGCAGCAA 
ACGCAGCAGG TCCAGTCTCC TGTGGACTCA GCCACAATGT CACCAGTGGA AAGGACAGCA 
GCGTGGGTTC TCAATAACGG GCAGTACGAG GAGGTCGAGG AGGACACAGA GCAGAACCCA 
GATGAAGCCA AGCATGCTGA GAAGTACGAA CAAGAGATAG CAAAGCTGAA GGAACGCTTG 
AAAGTGTCGA GCCGCCGGCT GGAGGAGTAC GAGCGGCGGC TCCTGGTGCA GGAGCAGCAA 
ATGCAGAAGC TGCTGATGGA GTACAAGTCC AGGCTGGAGG ACAGCGAGGA GAGACTGCGC 
AGGCAACAGG AGGAGAAGGA CAGCCAGATG AAAAGCATTA TCAGCAGGCT CATGGCAGTT 
GAAGAGGAAC TGAAGAAAGA TCATGCAGAG ATGCAAGCTG TCATCGATGC AAAGCAGAAA 
ATTATTGATG CACAGGTCAT TAATGGGAAT GAGATAATTC AAACAGGAGA AGCGGATCGT 
GTCCCTGGAC TCTGCCAACA CGCGGCTGAT GAGTGCCCTG ACGCAGGTGA AGGAGCGCTA 
CAGCATGCAA GTCCGCAATG GCATCTCCCC CACCAACCCC ACCAAGCTTT CCATCACGGA 
GAATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_015290520.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015146006.1 Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OGa152372
Clone ID Related Accession (Same CDS sequence)	XM_015290520.2
Accession Version	XM_015290520.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	ras GTPase-activating protein nGAP isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006095.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015290520.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCCGC CAAGCTGGGG CTTCTTCAGT AAACGTCTGA AAGGGTCCAT CAAGAGGACA 
AAGAGTCAGT CAAAGCTGGA CAGGAACACA AGTTTCCGCC TCCCATCCCT CCGCAATGCT 
GATGACAGGT CACGTGGGCT GCCGAAGCTG AAGGAGTCCC GCTCTCATGA GTCCCTGCTG 
AGCCCCGGCA GCGCAGTGGA AGCCCTGGAC CTTGGGGCAG AAGAAAAGGT CTTTGTAAAG 
CCACTGCACA GCAGCATCCT CGGACAGGAC TTCTGCTTTG AGGTAACCTA CTCCAGCGGC 
AGTAAATGCT TCAGCTGTTC CTCTGCTGCA GAGAGGGATA AGTGGATGGA AAACCTACGC 
AGAACAGTGC AGCCGAATAA GGACAACTGC CGTCGAGCTG AAAATGTGCT CCGTCTCTGG 
ATCATTGAGG CAAAGGACCT GGCCCCCAAG AAGAAATACT TCTGTGAGCT GTGCCTTGAT 
GACACTCTCT TTGCCCGCAC CACCAGCAAA ACGAAAGCAG ATAACATTTT CTGGGGGGAG 
CACTTTGAGT TCTACGGCCT CCCAGCTCTT CACAGCATCA CGGTTCACAT CTACAAGGAT 
GTGGAGAAGA AAAAGAAAAA GGACAAAAAC AATTACGTCG GCCTGGTCAA CATTCCCATG 
GCCACTGTAA CGGGGCGCCA GTTTGTGGAA AAATGGTACC CAGTCAGCAC ACCTACACCC 
AACAAAGGGA AATCAGGAGG CCCTTCCATT CGCATCAAGT CGCGTTATCA GACCATCACC 
ATCTTACCCA TGGAGCAATA CAAGGAGTTT GCAGAGTTTG TTACCAGCAA CTACACGATG 
CTGTGCTCTG TGCTGGAGCC AGTCATCAGC GTAAGGAATA AGGAAGAGAT GGCTTGTGCA 
ATGGTGCACA TTCTTCAGAG CACGGGGAGA GCCAAGGACT TCCTAACGGA TCTGGTGATG 
TCTGAGGTGG ATCGCTGCGG GGAACACGAT GTCTTGATCT TCAGGGAGAA CACACTTGCT 
ACCAAAGCTA TTGAGGAATA CCTGAAGTTG GTAGGGCAGA AGTATCTCCA TGATGCGCTA 
GGGGAGTTTA TCAAGGCTTT GTATGAGTCA GATGAAAACT GTGAAGTGGA CCCCAGCAAA 
TGTTCATCCA GTGAATTAAC AGACCATCAG AGCAACCTGA AAATGTGTTG TGAGCTGGCC 
TTCTGCAAGA TTATCAATTC CTACTGCGTG TTCCCTCGTG AGCTGAAGGA GGTGTTTGCA 
TCTTGGAAGC AGCAGTGCCT GAGCAGGGGC AAGCAGGACA TCAGCGAGCG GCTGATCAGT 
GCCTCCCTGT TCCTCCGCTT CCTGTGCCCC GCCATCATGT CCCCCAGCCT CTTCAGCCTC 
ATGCAGGAGT ATCCTGACGA CCGGACATCG CGCACGCTCA CACTCATTGC TAAAGTCATT 
CAGAATCTCG CCAATTTTGC TAAGTTTGGT AACAAGGAAG AGTACATGGC TTTCATGAAC 
GACTTTCTGG AACATGAGTG GGGGGGAATG AAGCGATTTC TCCTGGAGAT CTCTAATCCA 
GATACCATCT CAAACATGCC TGGATTTGAG GGCTATATCG ACCTGGGCAG AGAGCTCTCA 
GTTTTGCATT CCCTCTTATG GGAGGTTGTG TCCCAACTTG ATAAGGGTGA AAATTCCTTC 
CTACAGGCGA CCGTGGCAAA ACTGGGACCT CTTCCTCGTA TTCTTGCCGA CATCACCAAA 
TCAATGACCA ACCCTACGCC AATACAGCAG CAGCTGAGGC GTTTCACTGA GCACAGCTCT 
AGTCCAAACG TCAGTGGCAG TCTCTCCTCA GGGCTTCAGA AGATATTCGA GGACCCCACT 
GATGGTGACT TGCATAAACT GAAGTCTCCA ACCCAGGAAA ACATAGATGG CTATTTCAGG 
GGCAAGACCT TGCTGTTGGT TCAGCAGGCG TCCACCCAGA GCATGACTTA CTCAGATAAG 
GACGAAAGAG AGAGCATCTT GCCCAACGGA CGTAGCATCT CCCTCATGGA CCTGCAGGAT 
CCCCATACCG CTCACAGCGA CCACGCTTCC ATGATGCACG ACGTGCCTTT GCGCTTGGCC 
GGCAGCCAGC TCTCCATCAC CCAGGTGGCC AACATCAAGC AGCTGTGGGA GACCCAGAGC 
ACGCCCCAAA GCGCTCCCCA GGTGAGGAGG CCGCTGCACC CGGCTTTGAA CCAGCAGGGC 
AGCCTCCAGC CTCTGTCATT CCAGAACCCG GTGTACCACC TCAACAACCC ACCTGCGCCG 
TTGCCCAAGG CCTCCGTGGA CTCCAGCCTG GAGAACCTGA GCACCGCCAG CTCCCAGAGC 
CGAAGCAACA GCGAAGACTT CAAGCTCAGC GGGCCCAGCA ACAGCAGCAT GGAGGACTTC 
ACCAAGCGCA GCACGCAGAG CGAGGACTTC TCTAGGCGGC ACACGGTCCC GGACAAGCAC 
GTCCCCATCG CGTTGCCCCG CCAGAACAGC AGCAGCCAGG CACAGATCCG CAAAATGGAC 
CAGGCCGGGT TAGGGGCCAG GGCCAAAGCG CCGCAGTCGC TGCCGCACAG CGCGTCCCTG 
CGCAGCACGG GCAGCATGTC GGCCGTGTCG GGGGCCGTGG TGACTGAGCC CGTCCAGAAC 
GGCAGCCGCT CCCGGCAGCA GTCGTCCTCC TCCAGGGAGA GCCCTGTGCC GAAGGTGAGG 
GCCATTCAGA GGCAGCAAAC GCAGCAGGTC CAGTCTCCTG TGGACTCAGC CACAATGTCA 
CCAGTGGAAA GGACAGCAGC GTGGGTTCTC AATAACGGGC AGTACGAGGA GGTCGAGGAG 
GACACAGAGC AGAACCCAGA TGAAGCCAAG CATGCTGAGA AGTACGAACA AGAGATAGCA 
AAGCTGAAGG AACGCTTGAA AGTGTCGAGC CGCCGGCTGG AGGAGTACGA GCGGCGGCTC 
CTGGTGCAGG AGCAGCAAAT GCAGAAGCTG CTGATGGAGT ACAAGTCCAG GCTGGAGGAC 
AGCGAGGAGA GACTGCGCAG GCAACAGGAG GAGAAGGACA GCCAGATGAA AAGCATTATC 
AGCAGGCTCA TGGCAGTTGA AGAGGAACTG AAGAAAGATC ATGCAGAGAT GCAAGCTGTC 
ATCGATGCAA AGCAGAAAAT TATTGATGCA CAGGTCATTA ATGGGAATGA GATAATTCAA 
ACAGGAGAAG CGGATCGTGT CCCTGGACTC TGCCAACACG CGGCTGATGA GTGCCCTGAC 
GCAGGTGAAG GAGCGCTACA GCATGCAAGT CCGCAATGGC ATCTCCCCCA CCAACCCCAC 
CAAGCTTTCC ATCACGGAGA ATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015146006.1
CDS	253..3579
Translation	MGPPSWGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNADDRSRGLPKLKESRSHESLLSPGSAVEALDLGAEEKVFVKPLHSSILGQDFCFEVTYSSGSKCFSCSSAAERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYGLPALHSITVHIYKDVEKKKKKDKNNYVGLVNIPMATVTGRQFVEKWYPVSTPTPNKGKSGGPSIRIKSRYQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEEMACAMVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTLATKAIEEYLKLVGQKYLHDALGEFIKALYESDENCEVDPSKCSSSELTDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCLSRGKQDISERLISASLFLRFLCPAIMSPSLFSLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNMPGFEGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVAKLGPLPRILADITKSMTNPTPIQQQLRRFTEHSSSPNVSGSLSSGLQKIFEDPTDGDLHKLKSPTQENIDGYFRGKTLLLVQQASTQSMTYSDKDERESILPNGRSISLMDLQDPHTAHSDHASMMHDVPLRLAGSQLSITQVANIKQLWETQSTPQSAPQVRRPLHPALNQQGSLQPLSFQNPVYHLNNPPAPLPKASVDSSLENLSTASSQSRSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTVPDKHVPIALPRQNSSSQAQIRKMDQAGLGARAKAPQSLPHSASLRSTGSMSAVSGAVVTEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEVEEDTEQNPDEAKHAEKYEQEIAKLKERLKVSSRRLEEYERRLLVQEQQMQKLLMEYKSRLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQVINGNEIIQTGEADRVPGLCQHAADECPDAGEGALQHASPQWHLPHQPHQAFHHGEW

Target ORF information:

RefSeq Version	XM_015290520.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015290520.2

ORF Insert Sequence:

ATGGGGCCGC CAAGCTGGGG CTTCTTCAGT AAACGTCTGA AAGGGTCCAT CAAGAGGACA 
AAGAGTCAGT CAAAGCTGGA CAGGAACACA AGTTTCCGCC TCCCATCCCT CCGCAATGCT 
GATGACAGGT CACGTGGGCT GCCGAAGCTG AAGGAGTCCC GCTCTCATGA GTCCCTGCTG 
AGCCCCGGCA GCGCAGTGGA AGCCCTGGAC CTTGGGGCAG AAGAAAAGGT CTTTGTAAAG 
CCACTGCACA GCAGCATCCT CGGACAGGAC TTCTGCTTTG AGGTAACCTA CTCCAGCGGC 
AGTAAATGCT TCAGCTGTTC CTCTGCTGCA GAGAGGGATA AGTGGATGGA AAACCTACGC 
AGAACAGTGC AGCCGAATAA GGACAACTGC CGTCGAGCTG AAAATGTGCT CCGTCTCTGG 
ATCATTGAGG CAAAGGACCT GGCCCCCAAG AAGAAATACT TCTGTGAGCT GTGCCTTGAT 
GACACTCTCT TTGCCCGCAC CACCAGCAAA ACGAAAGCAG ATAACATTTT CTGGGGGGAG 
CACTTTGAGT TCTACGGCCT CCCAGCTCTT CACAGCATCA CGGTTCACAT CTACAAGGAT 
GTGGAGAAGA AAAAGAAAAA GGACAAAAAC AATTACGTCG GCCTGGTCAA CATTCCCATG 
GCCACTGTAA CGGGGCGCCA GTTTGTGGAA AAATGGTACC CAGTCAGCAC ACCTACACCC 
AACAAAGGGA AATCAGGAGG CCCTTCCATT CGCATCAAGT CGCGTTATCA GACCATCACC 
ATCTTACCCA TGGAGCAATA CAAGGAGTTT GCAGAGTTTG TTACCAGCAA CTACACGATG 
CTGTGCTCTG TGCTGGAGCC AGTCATCAGC GTAAGGAATA AGGAAGAGAT GGCTTGTGCA 
ATGGTGCACA TTCTTCAGAG CACGGGGAGA GCCAAGGACT TCCTAACGGA TCTGGTGATG 
TCTGAGGTGG ATCGCTGCGG GGAACACGAT GTCTTGATCT TCAGGGAGAA CACACTTGCT 
ACCAAAGCTA TTGAGGAATA CCTGAAGTTG GTAGGGCAGA AGTATCTCCA TGATGCGCTA 
GGGGAGTTTA TCAAGGCTTT GTATGAGTCA GATGAAAACT GTGAAGTGGA CCCCAGCAAA 
TGTTCATCCA GTGAATTAAC AGACCATCAG AGCAACCTGA AAATGTGTTG TGAGCTGGCC 
TTCTGCAAGA TTATCAATTC CTACTGCGTG TTCCCTCGTG AGCTGAAGGA GGTGTTTGCA 
TCTTGGAAGC AGCAGTGCCT GAGCAGGGGC AAGCAGGACA TCAGCGAGCG GCTGATCAGT 
GCCTCCCTGT TCCTCCGCTT CCTGTGCCCC GCCATCATGT CCCCCAGCCT CTTCAGCCTC 
ATGCAGGAGT ATCCTGACGA CCGGACATCG CGCACGCTCA CACTCATTGC TAAAGTCATT 
CAGAATCTCG CCAATTTTGC TAAGTTTGGT AACAAGGAAG AGTACATGGC TTTCATGAAC 
GACTTTCTGG AACATGAGTG GGGGGGAATG AAGCGATTTC TCCTGGAGAT CTCTAATCCA 
GATACCATCT CAAACATGCC TGGATTTGAG GGCTATATCG ACCTGGGCAG AGAGCTCTCA 
GTTTTGCATT CCCTCTTATG GGAGGTTGTG TCCCAACTTG ATAAGGGTGA AAATTCCTTC 
CTACAGGCGA CCGTGGCAAA ACTGGGACCT CTTCCTCGTA TTCTTGCCGA CATCACCAAA 
TCAATGACCA ACCCTACGCC AATACAGCAG CAGCTGAGGC GTTTCACTGA GCACAGCTCT 
AGTCCAAACG TCAGTGGCAG TCTCTCCTCA GGGCTTCAGA AGATATTCGA GGACCCCACT 
GATGGTGACT TGCATAAACT GAAGTCTCCA ACCCAGGAAA ACATAGATGG CTATTTCAGG 
GGCAAGACCT TGCTGTTGGT TCAGCAGGCG TCCACCCAGA GCATGACTTA CTCAGATAAG 
GACGAAAGAG AGAGCATCTT GCCCAACGGA CGTAGCATCT CCCTCATGGA CCTGCAGGAT 
CCCCATACCG CTCACAGCGA CCACGCTTCC ATGATGCACG ACGTGCCTTT GCGCTTGGCC 
GGCAGCCAGC TCTCCATCAC CCAGGTGGCC AACATCAAGC AGCTGTGGGA GACCCAGAGC 
ACGCCCCAAA GCGCTCCCCA GGTGAGGAGG CCGCTGCACC CGGCTTTGAA CCAGCAGGGC 
AGCCTCCAGC CTCTGTCATT CCAGAACCCG GTGTACCACC TCAACAACCC ACCTGCGCCG 
TTGCCCAAGG CCTCCGTGGA CTCCAGCCTG GAGAACCTGA GCACCGCCAG CTCCCAGAGC 
CGAAGCAACA GCGAAGACTT CAAGCTCAGC GGGCCCAGCA ACAGCAGCAT GGAGGACTTC 
ACCAAGCGCA GCACGCAGAG CGAGGACTTC TCTAGGCGGC ACACGGTCCC GGACAAGCAC 
GTCCCCATCG CGTTGCCCCG CCAGAACAGC AGCAGCCAGG CACAGATCCG CAAAATGGAC 
CAGGCCGGGT TAGGGGCCAG GGCCAAAGCG CCGCAGTCGC TGCCGCACAG CGCGTCCCTG 
CGCAGCACGG GCAGCATGTC GGCCGTGTCG GGGGCCGTGG TGACTGAGCC CGTCCAGAAC 
GGCAGCCGCT CCCGGCAGCA GTCGTCCTCC TCCAGGGAGA GCCCTGTGCC GAAGGTGAGG 
GCCATTCAGA GGCAGCAAAC GCAGCAGGTC CAGTCTCCTG TGGACTCAGC CACAATGTCA 
CCAGTGGAAA GGACAGCAGC GTGGGTTCTC AATAACGGGC AGTACGAGGA GGTCGAGGAG 
GACACAGAGC AGAACCCAGA TGAAGCCAAG CATGCTGAGA AGTACGAACA AGAGATAGCA 
AAGCTGAAGG AACGCTTGAA AGTGTCGAGC CGCCGGCTGG AGGAGTACGA GCGGCGGCTC 
CTGGTGCAGG AGCAGCAAAT GCAGAAGCTG CTGATGGAGT ACAAGTCCAG GCTGGAGGAC 
AGCGAGGAGA GACTGCGCAG GCAACAGGAG GAGAAGGACA GCCAGATGAA AAGCATTATC 
AGCAGGCTCA TGGCAGTTGA AGAGGAACTG AAGAAAGATC ATGCAGAGAT GCAAGCTGTC 
ATCGATGCAA AGCAGAAAAT TATTGATGCA CAGGTCATTA ATGGGAATGA GATAATTCAA 
ACAGGAGAAG CGGATCGTGT CCCTGGACTC TGCCAACACG CGGCTGATGA GTGCCCTGAC 
GCAGGTGAAG GAGCGCTACA GCATGCAAGT CCGCAATGGC ATCTCCCCCA CCAACCCCAC 
CAAGCTTTCC ATCACGGAGA ATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_015290510.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015145996.1 Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X1, mRNA.

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CloneID	OGa39039
Clone ID Related Accession (Same CDS sequence)	XM_015290510.1
Accession Version	XM_015290510.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	ras GTPase-activating protein nGAP isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGCTT TTAAAAAGTA TTACAGTCTC TCTCCCAGGA CCTTTAGGAG GTCTTTGTCG 
GAGCAAGATG CTGGCACCGC TGCCTTAAAG GACTATAATT GGTACCACCA AATGAGAAGC 
AAGAAGGGGA GCAAACAAAC AGCTTTGGAC CGAGAGAAAG CACTCGGAGC GTACAGGTGG 
CAAACACATC CCCGTCTTCC CTGGAGGACA AGCAGAAGAG CACACTCTTG CAGCCTCAGA 
GGGGAAGGTA ATGATTCGAG TCTTGTCAAA AGTTCTGCAA GCATGGATGC TACCAGCGCC 
GACGATCTCC ACCAAGACTC AACGGAGATG CTCTTCCAGG CAAGGACCAG ATCACCGCGG 
GTTCCCTTCA ACCGCTCCCT GTCTGAACCT GTGCCGCACT CTGCAAACAG GAATGCCAAG 
CCCTTTCTGC AGGCGGTGCG CTCTGTGGAT TGTGAGCAGC ACAGCTATTT TGTCCGTGGC 
ATCTTCTCAA CTCTCTCAAG TGGCTTTTCA AAGATAATAA ATCGAAGGGG TGAGCAGCCC 
CCCACTGAGC CACTGGAGGA GTGGAAACCA GCCGGTAGTC AAATTGACCC GTGCCCAGGT 
AATGGACTCT GTTCTGTTTG TGTTACAGAT GTAAAAGGAC CACCTACGCA CAGGCTGTCC 
TGCGGCCAGT CCCCCTACAC CGAGACGACA ACATGGGAGA GGAAGTACTG CATCCTGACT 
GACAGCCAGC TGGTGCTGCT CAACAGGGAG AAGGAGGTGC CCACCGAGGG GTTGCAGGAG 
CCGCAGGATG CAACCAAAGG CCGCTGTCTG CGCCGCACGG TGAGCGTGCC CTCCGAGGGC 
CAGTTCCCCG AGTACCCACC AGATGGGGCC GCCAAGCTGG AGGTCCCGGC AGAGAGATCT 
CCTCGCAGAC GGAGCATCTC TGGGACCAGC ACATCTGAGA AAACCAACTC CATGGATGCT 
GCAAATACCT CTCCTTTCAA AGTATCGGGC TTCTTCAGTA AACGTCTGAA AGGGTCCATC 
AAGAGGACAA AGAGTCAGTC AAAGCTGGAC AGGAACACAA GTTTCCGCCT CCCATCCCTC 
CGCAATGCTG ATGACAGGTC ACGTGGGCTG CCGAAGCTGA AGGAGTCCCG CTCTCATGAG 
TCCCTGCTGA GCCCCGGCAG CGCAGTGGAA GCCCTGGACC TTGGGGCAGA AGAAAAGGTC 
TTTGTAAAGC CACTGCACAG CAGCATCCTC GGACAGGACT TCTGCTTTGA GGTAACCTAC 
TCCAGCGGCA GTAAATGCTT CAGCTGTTCC TCTGCTGCAG AGAGGGATAA GTGGATGGAA 
AACCTACGCA GAACAGTGCA GCCGAATAAG GACAACTGCC GTCGAGCTGA AAATGTGCTC 
CGTCTCTGGA TCATTGAGGC AAAGGACCTG GCCCCCAAGA AGAAATACTT CTGTGAGCTG 
TGCCTTGATG ACACTCTCTT TGCCCGCACC ACCAGCAAAA CGAAAGCAGA TAACATTTTC 
TGGGGGGAGC ACTTTGAGTT CTACGGCCTC CCAGCTCTTC ACAGCATCAC GGTTCACATC 
TACAAGGATG TGGAGAAGAA AAAGAAAAAG GACAAAAACA ATTACGTCGG CCTGGTCAAC 
ATTCCCATGG CCACTGTAAC GGGGCGCCAG TTTGTGGAAA AATGGTACCC AGTCAGCACA 
CCTACACCCA ACAAAGGGAA ATCAGGAGGC CCTTCCATTC GCATCAAGTC GCGTTATCAG 
ACCATCACCA TCTTACCCAT GGAGCAATAC AAGGAGTTTG CAGAGTTTGT TACCAGCAAC 
TACACGATGC TGTGCTCTGT GCTGGAGCCA GTCATCAGCG TAAGGAATAA GGAAGAGATG 
GCTTGTGCAA TGGTGCACAT TCTTCAGAGC ACGGGGAGAG CCAAGGACTT CCTAACGGAT 
CTGGTGATGT CTGAGGTGGA TCGCTGCGGG GAACACGATG TCTTGATCTT CAGGGAGAAC 
ACACTTGCTA CCAAAGCTAT TGAGGAATAC CTGAAGTTGG TAGGGCAGAA GTATCTCCAT 
GATGCGCTAG GGGAGTTTAT CAAGGCTTTG TATGAGTCAG ATGAAAACTG TGAAGTGGAC 
CCCAGCAAAT GTTCATCCAG TGAATTAACA GACCATCAGA GCAACCTGAA AATGTGTTGT 
GAGCTGGCCT TCTGCAAGAT TATCAATTCC TACTGCGTGT TCCCTCGTGA GCTGAAGGAG 
GTGTTTGCAT CTTGGAAGCA GCAGTGCCTG AGCAGGGGCA AGCAGGACAT CAGCGAGCGG 
CTGATCAGTG CCTCCCTGTT CCTCCGCTTC CTCTGCCCCG CCATCATGTC CCCCAGCCTC 
TTCAGCCTCA TGCAGGAGTA TCCTGACGAC CGGACATCGC GCACGCTCAC ACTCATTGCT 
AAAGTCATTC AGAATCTCGC CAATTTTGCT AAGTTTGGTA ACAAGGAAGA GTACATGGCT 
TTCATGAACG ACTTTCTGGA ACATGAGTGG GGGGGAATGA AGCGATTTCT CCTGGAGATC 
TCTAATCCAG ATACCATCTC AAACATGCCT GGATTTGAGG GCTATATCGA CCTGGGCAGA 
GAGCTCTCAG TTTTGCATTC CCTCTTATGG GAGGTTGTGT CCCAACTTGA TAAGGGTGAA 
AATTCCTTCC TACAGGCGAC CGTGGCAAAA CTGGGACCTC TTCCTCGTAT TCTTGCCGAC 
ATCACCAAAT CAATGACCAA CCCTACGCCA ATACAGCAGC AGCTGAGGCG TTTCACTGAG 
CACAGCTCTA GTCCAAACGT CAGTGGCAGT CTCTCCTCAG GGCTTCAGAA GATATTCGAG 
GACCCCACTG ATGGTGACTT GCATAAACTG AAGTCTCCAA CCCAGGAAAA CATAGATGGC 
TATTTCAGGG GCAAGACCTT GCTGTTGGTT CAGCAGGCGT CCACCCAGAG CATGACTTAC 
TCAGATAAGG ACGAAAGAGA GAGCATCTTG CCCAACGGAC GTAGCATCTC CCTCATGGAC 
CTGCAGGATC CCCATACCGC TCACAGCGAC CACGCTTCCA TGATGCACGA CGTGCCTTTG 
CGCTTGGCCG GCAGCCAGCT CTCCATCACC CAGGTGGCCA ACATCAAGCA GCTGTGGGAG 
ACCCAGAGCA CGCCCCAAAG CGCTCCCCAG GTGAGGAGGC CGCTGCACCC GGCTTTGAAC 
CAGCAGGGCA GCCTCCAGCC TCTGTCATTC CAGAACCCGG TGTACCACCT CAACAACCCA 
CCTGCGCCGT TGCCCAAGGC CTCCGTGGAC TCCAGCCTGG AGAACCTGAG CACCGCCAGC 
TCCCAGAGCC GAAGCAACAG CGAAGACTTC AAGCTCAGCG GGCCCAGCAA CAGCAGCATG 
GAGGACTTCA CCAAGCGCAG CACGCAGAGC GAGGACTTCT CTAGGCGGCA CACGGTCCCG 
GACAAGCACG TCCCCATCGC GTTGCCCCGC CAGAACAGCA GCAGCCAGGC ACAGATCCGC 
AAAATGGACC AGGCCGGGTT AGGGGCCAGG GCCAAAGCGC CGCAGTCGCT GCCGCACAGC 
GCGTCCCTGC GCAGCACGGG CAGCATGTCG GCCGTGTCGG GGGCCGTGGT GACTGAGCCC 
GTCCAGAACG GCAGCCGCTC CCGGCAGCAG TCGTCCTCCT CCAGGGAGAG CCCTGTGCCG 
AAGGTGAGGG CCATTCAGAG GCAGCAAACG CAGCAGGTCC AGTCTCCTGT GGACTCAGCC 
ACAATGTCAC CAGTGGAAAG GACAGCAGCG TGGGTTCTCA ATAACGGGCA GTACGAGGAG 
GTCGAGGAGG ACACAGAGCA GAACCCAGAT GAAGCCAAGC ATGCTGAGAA GTACGAACAA 
GAGATAGCAA AGCTGAAGGA ACGCTTGAAA GTGTCGAGCC GCCGGCTGGA GGAGTACGAG 
CGGCGGCTCC TGGTGCAGGA GCAGCAAATG CAGAAGCTGC TGATGGAGTA CAAGTCCAGG 
CTGGAGGACA GCGAGGAGAG ACTGCGCAGG CAACAGGAGG AGAAGGACAG CCAGATGAAA 
AGCATTATCA GCAGGCTCAT GGCAGTTGAA GAGGAACTGA AGAAAGATCA TGCAGAGATG 
CAAGCTGTCA TCGATGCAAA GCAGAAAATT ATTGATGCAC AGGTCATTAA TGGGAATGAG 
ATAATTCAAA CAGGAGAAGC GGATCGTGTC CCTGGACTCT GCCAACACGC GGCTGATGAG 
TGCCCTGACG CAGGTGAAGG AGCGCTACAG CATGCAAGTC CGCAATGGCA TCTCCCCCAC 
CAACCCCACC AAGCTTTCCA TCACGGAGAA TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015145996.1
CDS	638..4933
Translation	MSAFKKYYSLSPRTFRRSLSEQDAGTAALKDYNWYHQMRSKKGSKQTALDREKALGAYRWQTHPRLPWRTSRRAHSCSLRGEGNDSSLVKSSASMDATSADDLHQDSTEMLFQARTRSPRVPFNRSLSEPVPHSANRNAKPFLQAVRSVDCEQHSYFVRGIFSTLSSGFSKIINRRGEQPPTEPLEEWKPAGSQIDPCPGNGLCSVCVTDVKGPPTHRLSCGQSPYTETTTWERKYCILTDSQLVLLNREKEVPTEGLQEPQDATKGRCLRRTVSVPSEGQFPEYPPDGAAKLEVPAERSPRRRSISGTSTSEKTNSMDAANTSPFKVSGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNADDRSRGLPKLKESRSHESLLSPGSAVEALDLGAEEKVFVKPLHSSILGQDFCFEVTYSSGSKCFSCSSAAERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYGLPALHSITVHIYKDVEKKKKKDKNNYVGLVNIPMATVTGRQFVEKWYPVSTPTPNKGKSGGPSIRIKSRYQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEEMACAMVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTLATKAIEEYLKLVGQKYLHDALGEFIKALYESDENCEVDPSKCSSSELTDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCLSRGKQDISERLISASLFLRFLCPAIMSPSLFSLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNMPGFEGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVAKLGPLPRILADITKSMTNPTPIQQQLRRFTEHSSSPNVSGSLSSGLQKIFEDPTDGDLHKLKSPTQENIDGYFRGKTLLLVQQASTQSMTYSDKDERESILPNGRSISLMDLQDPHTAHSDHASMMHDVPLRLAGSQLSITQVANIKQLWETQSTPQSAPQVRRPLHPALNQQGSLQPLSFQNPVYHLNNPPAPLPKASVDSSLENLSTASSQSRSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTVPDKHVPIALPRQNSSSQAQIRKMDQAGLGARAKAPQSLPHSASLRSTGSMSAVSGAVVTEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEVEEDTEQNPDEAKHAEKYEQEIAKLKERLKVSSRRLEEYERRLLVQEQQMQKLLMEYKSRLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQVINGNEIIQTGEADRVPGLCQHAADECPDAGEGALQHASPQWHLPHQPHQAFHHGEW

Target ORF information:

RefSeq Version	XM_015290510.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015290510.1

ORF Insert Sequence:

ATGAGTGCTT TTAAAAAGTA TTACAGTCTC TCTCCCAGGA CCTTTAGGAG GTCTTTGTCG 
GAGCAAGATG CTGGCACCGC TGCCTTAAAG GACTATAATT GGTACCACCA AATGAGAAGC 
AAGAAGGGGA GCAAACAAAC AGCTTTGGAC CGAGAGAAAG CACTCGGAGC GTACAGGTGG 
CAAACACATC CCCGTCTTCC CTGGAGGACA AGCAGAAGAG CACACTCTTG CAGCCTCAGA 
GGGGAAGGTA ATGATTCGAG TCTTGTCAAA AGTTCTGCAA GCATGGATGC TACCAGCGCC 
GACGATCTCC ACCAAGACTC AACGGAGATG CTCTTCCAGG CAAGGACCAG ATCACCGCGG 
GTTCCCTTCA ACCGCTCCCT GTCTGAACCT GTGCCGCACT CTGCAAACAG GAATGCCAAG 
CCCTTTCTGC AGGCGGTGCG CTCTGTGGAT TGTGAGCAGC ACAGCTATTT TGTCCGTGGC 
ATCTTCTCAA CTCTCTCAAG TGGCTTTTCA AAGATAATAA ATCGAAGGGG TGAGCAGCCC 
CCCACTGAGC CACTGGAGGA GTGGAAACCA GCCGGTAGTC AAATTGACCC GTGCCCAGGT 
AATGGACTCT GTTCTGTTTG TGTTACAGAT GTAAAAGGAC CACCTACGCA CAGGCTGTCC 
TGCGGCCAGT CCCCCTACAC CGAGACGACA ACATGGGAGA GGAAGTACTG CATCCTGACT 
GACAGCCAGC TGGTGCTGCT CAACAGGGAG AAGGAGGTGC CCACCGAGGG GTTGCAGGAG 
CCGCAGGATG CAACCAAAGG CCGCTGTCTG CGCCGCACGG TGAGCGTGCC CTCCGAGGGC 
CAGTTCCCCG AGTACCCACC AGATGGGGCC GCCAAGCTGG AGGTCCCGGC AGAGAGATCT 
CCTCGCAGAC GGAGCATCTC TGGGACCAGC ACATCTGAGA AAACCAACTC CATGGATGCT 
GCAAATACCT CTCCTTTCAA AGTATCGGGC TTCTTCAGTA AACGTCTGAA AGGGTCCATC 
AAGAGGACAA AGAGTCAGTC AAAGCTGGAC AGGAACACAA GTTTCCGCCT CCCATCCCTC 
CGCAATGCTG ATGACAGGTC ACGTGGGCTG CCGAAGCTGA AGGAGTCCCG CTCTCATGAG 
TCCCTGCTGA GCCCCGGCAG CGCAGTGGAA GCCCTGGACC TTGGGGCAGA AGAAAAGGTC 
TTTGTAAAGC CACTGCACAG CAGCATCCTC GGACAGGACT TCTGCTTTGA GGTAACCTAC 
TCCAGCGGCA GTAAATGCTT CAGCTGTTCC TCTGCTGCAG AGAGGGATAA GTGGATGGAA 
AACCTACGCA GAACAGTGCA GCCGAATAAG GACAACTGCC GTCGAGCTGA AAATGTGCTC 
CGTCTCTGGA TCATTGAGGC AAAGGACCTG GCCCCCAAGA AGAAATACTT CTGTGAGCTG 
TGCCTTGATG ACACTCTCTT TGCCCGCACC ACCAGCAAAA CGAAAGCAGA TAACATTTTC 
TGGGGGGAGC ACTTTGAGTT CTACGGCCTC CCAGCTCTTC ACAGCATCAC GGTTCACATC 
TACAAGGATG TGGAGAAGAA AAAGAAAAAG GACAAAAACA ATTACGTCGG CCTGGTCAAC 
ATTCCCATGG CCACTGTAAC GGGGCGCCAG TTTGTGGAAA AATGGTACCC AGTCAGCACA 
CCTACACCCA ACAAAGGGAA ATCAGGAGGC CCTTCCATTC GCATCAAGTC GCGTTATCAG 
ACCATCACCA TCTTACCCAT GGAGCAATAC AAGGAGTTTG CAGAGTTTGT TACCAGCAAC 
TACACGATGC TGTGCTCTGT GCTGGAGCCA GTCATCAGCG TAAGGAATAA GGAAGAGATG 
GCTTGTGCAA TGGTGCACAT TCTTCAGAGC ACGGGGAGAG CCAAGGACTT CCTAACGGAT 
CTGGTGATGT CTGAGGTGGA TCGCTGCGGG GAACACGATG TCTTGATCTT CAGGGAGAAC 
ACACTTGCTA CCAAAGCTAT TGAGGAATAC CTGAAGTTGG TAGGGCAGAA GTATCTCCAT 
GATGCGCTAG GGGAGTTTAT CAAGGCTTTG TATGAGTCAG ATGAAAACTG TGAAGTGGAC 
CCCAGCAAAT GTTCATCCAG TGAATTAACA GACCATCAGA GCAACCTGAA AATGTGTTGT 
GAGCTGGCCT TCTGCAAGAT TATCAATTCC TACTGCGTGT TCCCTCGTGA GCTGAAGGAG 
GTGTTTGCAT CTTGGAAGCA GCAGTGCCTG AGCAGGGGCA AGCAGGACAT CAGCGAGCGG 
CTGATCAGTG CCTCCCTGTT CCTCCGCTTC CTCTGCCCCG CCATCATGTC CCCCAGCCTC 
TTCAGCCTCA TGCAGGAGTA TCCTGACGAC CGGACATCGC GCACGCTCAC ACTCATTGCT 
AAAGTCATTC AGAATCTCGC CAATTTTGCT AAGTTTGGTA ACAAGGAAGA GTACATGGCT 
TTCATGAACG ACTTTCTGGA ACATGAGTGG GGGGGAATGA AGCGATTTCT CCTGGAGATC 
TCTAATCCAG ATACCATCTC AAACATGCCT GGATTTGAGG GCTATATCGA CCTGGGCAGA 
GAGCTCTCAG TTTTGCATTC CCTCTTATGG GAGGTTGTGT CCCAACTTGA TAAGGGTGAA 
AATTCCTTCC TACAGGCGAC CGTGGCAAAA CTGGGACCTC TTCCTCGTAT TCTTGCCGAC 
ATCACCAAAT CAATGACCAA CCCTACGCCA ATACAGCAGC AGCTGAGGCG TTTCACTGAG 
CACAGCTCTA GTCCAAACGT CAGTGGCAGT CTCTCCTCAG GGCTTCAGAA GATATTCGAG 
GACCCCACTG ATGGTGACTT GCATAAACTG AAGTCTCCAA CCCAGGAAAA CATAGATGGC 
TATTTCAGGG GCAAGACCTT GCTGTTGGTT CAGCAGGCGT CCACCCAGAG CATGACTTAC 
TCAGATAAGG ACGAAAGAGA GAGCATCTTG CCCAACGGAC GTAGCATCTC CCTCATGGAC 
CTGCAGGATC CCCATACCGC TCACAGCGAC CACGCTTCCA TGATGCACGA CGTGCCTTTG 
CGCTTGGCCG GCAGCCAGCT CTCCATCACC CAGGTGGCCA ACATCAAGCA GCTGTGGGAG 
ACCCAGAGCA CGCCCCAAAG CGCTCCCCAG GTGAGGAGGC CGCTGCACCC GGCTTTGAAC 
CAGCAGGGCA GCCTCCAGCC TCTGTCATTC CAGAACCCGG TGTACCACCT CAACAACCCA 
CCTGCGCCGT TGCCCAAGGC CTCCGTGGAC TCCAGCCTGG AGAACCTGAG CACCGCCAGC 
TCCCAGAGCC GAAGCAACAG CGAAGACTTC AAGCTCAGCG GGCCCAGCAA CAGCAGCATG 
GAGGACTTCA CCAAGCGCAG CACGCAGAGC GAGGACTTCT CTAGGCGGCA CACGGTCCCG 
GACAAGCACG TCCCCATCGC GTTGCCCCGC CAGAACAGCA GCAGCCAGGC ACAGATCCGC 
AAAATGGACC AGGCCGGGTT AGGGGCCAGG GCCAAAGCGC CGCAGTCGCT GCCGCACAGC 
GCGTCCCTGC GCAGCACGGG CAGCATGTCG GCCGTGTCGG GGGCCGTGGT GACTGAGCCC 
GTCCAGAACG GCAGCCGCTC CCGGCAGCAG TCGTCCTCCT CCAGGGAGAG CCCTGTGCCG 
AAGGTGAGGG CCATTCAGAG GCAGCAAACG CAGCAGGTCC AGTCTCCTGT GGACTCAGCC 
ACAATGTCAC CAGTGGAAAG GACAGCAGCG TGGGTTCTCA ATAACGGGCA GTACGAGGAG 
GTCGAGGAGG ACACAGAGCA GAACCCAGAT GAAGCCAAGC ATGCTGAGAA GTACGAACAA 
GAGATAGCAA AGCTGAAGGA ACGCTTGAAA GTGTCGAGCC GCCGGCTGGA GGAGTACGAG 
CGGCGGCTCC TGGTGCAGGA GCAGCAAATG CAGAAGCTGC TGATGGAGTA CAAGTCCAGG 
CTGGAGGACA GCGAGGAGAG ACTGCGCAGG CAACAGGAGG AGAAGGACAG CCAGATGAAA 
AGCATTATCA GCAGGCTCAT GGCAGTTGAA GAGGAACTGA AGAAAGATCA TGCAGAGATG 
CAAGCTGTCA TCGATGCAAA GCAGAAAATT ATTGATGCAC AGGTCATTAA TGGGAATGAG 
ATAATTCAAA CAGGAGAAGC GGATCGTGTC CCTGGACTCT GCCAACACGC GGCTGATGAG 
TGCCCTGACG CAGGTGAAGG AGCGCTACAG CATGCAAGTC CGCAATGGCA TCTCCCCCAC 
CAACCCCACC AAGCTTTCCA TCACGGAGAA TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_004943245.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004943302.2 Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa39040
Clone ID Related Accession (Same CDS sequence)	XM_004943245.2
Accession Version	XM_004943245.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4287bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	ras GTPase-activating protein nGAP isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004943245.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGCTT TTAAAAAGTA TTACAGTCTC TCTCCCAGGA CCTTTAGGAG GTCTTTGTCG 
GAGCAAGATG CTGGCACCGC TGCCTTAAAG GACTATAATT GGTACCACCA AATGAGAAGC 
AAGAAGGGGA GCAAACAAAC AGCTTTGGAC CGAGAGAAAG CACTCGGAGC GTACAGGTGG 
CAAACACATC CCCGTCTTCC CTGGAGGACA AGCAGAAGAG CACACTCTTG CAGCCTCAGA 
GGGGAAGGTA ATGATTCGAG TCTTGTCAAA AGTTCTGCAA GCATGGATGC TACCAGCGCC 
GACGATCTCC ACCAAGACTC AACGGAGATG CTCTTCCAGG CAAGGACCAG ATCACCGCGG 
GTTCCCTTCA ACCGCTCCCT GTCTGAACCT GTGCCGCACT CTGCAAACAG GAATGCCAAG 
CCCTTTCTGC AGGCGGTGCG CTCTGTGGAT TGTGAGCAGC ACAGCTATTT TGTCCGTGGC 
ATCTTCTCAA CTCTCTCAAG TGGCTTTTCA AAGATAATAA ATCGAAGGGG TGAGCAGCCC 
CCCACTGAGC CACTGGAGGA GTGGAAACCA GCCGGTAGTC AAATTGACCC GTGCCCAGGT 
AATGGACTCT GTTCTGTTTG TGTTACAGAT GTAAAAGGAC CACCTACGCA CAGGCTGTCC 
TGCGGCCAGT CCCCCTACAC CGAGACGACA ACATGGGAGA GGAAGTACTG CATCCTGACT 
GACAGCCAGC TGGTGCTGCT CAACAGGGAG AAGGAGGTGC CCACCGAGGG GTTGCAGGAG 
CCGCAGGATG CAACCAAAGG CCGCTGTCTG CGCCGCACGG TGAGCGTGCC CTCCGAGGGC 
CAGTTCCCCG AGTACCCACC AGATGGGGCC GCCAAGCTGG AGGTCCCGGC AGAGAGATCT 
CCTCGCAGAC GGAGCATCTC TGGGACCAGC ACATCTGAGA AAACCAACTC CATGGATGCT 
GCAAATACCT CTCCTTTCAA AGTATCGGGC TTCTTCAGTA AACGTCTGAA AGGGTCCATC 
AAGAGGACAA AGAGTCAGTC AAAGCTGGAC AGGAACACAA GTTTCCGCCT CCCATCCCTC 
CGCAATGCTG ATGACAGGTC ACGTGGGCTG CCGAAGCTGA AGGAGTCCCG CTCTCATGAG 
TCCCTGCTGA GCCCCGGCAG CGCAGTGGAA GCCCTGGACC TTGGGGCAGA AGAAAAGGTC 
TTTGTAAAGC CACTGCACAG CAGCATCCTC GGACAGGACT TCTGCTTTGA GGTAACCTAC 
TCCAGCGGCA GTAAATGCTT CAGCTGTTCC TCTGCTGCAG AGAGGGATAA GTGGATGGAA 
AACCTACGCA GAACAGTGCA GCCGAATAAG GACAACTGCC GTCGAGCTGA AAATGTGCTC 
CGTCTCTGGA TCATTGAGGC AAAGGACCTG GCCCCCAAGA AGAAATACTT CTGTGAGCTG 
TGCCTTGATG ACACTCTCTT TGCCCGCACC ACCAGCAAAA CGAAAGCAGA TAACATTTTC 
TGGGGGGAGC ACTTTGAGTT CTACGGCCTC CCAGCTCTTC ACAGCATCAC GGTTCACATC 
TACAAGGATG TGGAGAAGAA AAAGAAAAAG GACAAAAACA ATTACGTCGG CCTGGTCAAC 
ATTCCCATGG CCACTGTAAC GGGGCGCCAG TTTGTGGAAA AATGGTACCC AGTCAGCACA 
CCTACACCCA ACAAAGGGAA ATCAGGAGGC CCTTCCATTC GCATCAAGTC GCGTTATCAG 
ACCATCACCA TCTTACCCAT GGAGCAATAC AAGGAGTTTG CAGAGTTTGT TACCAGCAAC 
TACACGATGC TGTGCTCTGT GCTGGAGCCA GTCATCAGCG TAAGGAATAA GGAAGAGATG 
GCTTGTGCAA TGGTGCACAT TCTTCAGAGC ACGGGGAGAG CCAAGGACTT CCTAACGGAT 
CTGGTGATGT CTGAGGTGGA TCGCTGCGGG GAACACGATG TCTTGATCTT CAGGGAGAAC 
ACACTTGCTA CCAAAGCTAT TGAGGAATAC CTGAAGTTGG TAGGGCAGAA GTATCTCCAT 
GATGCGCTAG GGGAGTTTAT CAAGGCTTTG TATGAGTCAG ATGAAAACTG TGAAGTGGAC 
CCCAGCAAAT GTTCATCCAG TGAATTAACA GACCATCAGA GCAACCTGAA AATGTGTTGT 
GAGCTGGCCT TCTGCAAGAT TATCAATTCC TACTGCGTGT TCCCTCGTGA GCTGAAGGAG 
GTGTTTGCAT CTTGGAAGCA GCAGTGCCTG AGCAGGGGCA AGCAGGACAT CAGCGAGCGG 
CTGATCAGTG CCTCCCTGTT CCTCCGCTTC CTCTGCCCCG CCATCATGTC CCCCAGCCTC 
TTCAGCCTCA TGCAGGAGTA TCCTGACGAC CGGACATCGC GCACGCTCAC ACTCATTGCT 
AAAGTCATTC AGAATCTCGC CAATTTTGCT AAGTTTGGTA ACAAGGAAGA GTACATGGCT 
TTCATGAACG ACTTTCTGGA ACATGAGTGG GGGGGAATGA AGCGATTTCT CCTGGAGATC 
TCTAATCCAG ATACCATCTC AAACATGCCT GGATTTGAGG GCTATATCGA CCTGGGCAGA 
GAGCTCTCAG TTTTGCATTC CCTCTTATGG GAGGTTGTGT CCCAACTTGA TAAGGGTGAA 
AATTCCTTCC TACAGGCGAC CGTGGCAAAA CTGGGACCTC TTCCTCGTAT TCTTGCCGAC 
ATCACCAAAT CAATGACCAA CCCTACGCCA ATACAGCAGC AGCTGAGGCG TTTCACTGAG 
CACAGCTCTA GTCCAAACGT CAGTGGCAGT CTCTCCTCAG GGCTTCAGAA GATATTCGAG 
GACCCCACTG ATGGTGACTT GCATAAACTG AAGTCTCCAA CCCAGGAAAA CATAGATGGC 
TATTTCAGGG GCAAGACCTT GCTGTTGGTT CAGCAGGCGT CCACCCAGAG CATGACTTAC 
TCAGATAAGG ACGAAAGAGA GAGCATCTTG CCCAACGGAC GTAGCATCTC CCTCATGGAC 
CTGCAGGATC CCCATACCGC TCACAGCGAC CACGCTTCCA TGATGCACGA CGTGCCTTTG 
CGCTTGGCCG GCAGCCAGCT CTCCATCACC CAGGTGGCCA ACATCAAGCA GCTGTGGGAG 
ACCCAGAGCA CGCCCCAAAG CGCTCCCCAG GTGAGGAGGC CGCTGCACCC GGCTTTGAAC 
CAGCAGGGCA GCCTCCAGCC TCTGTCATTC CAGAACCCGG TGTACCACCT CAACAACCCA 
CCTGCGCCGT TGCCCAAGGC CTCCGTGGAC TCCAGCCTGG AGAACCTGAG CACCGCCAGC 
TCCCAGAGCC GAAGCAACAG CGAAGACTTC AAGCTCAGCG GGCCCAGCAA CAGCAGCATG 
GAGGACTTCA CCAAGCGCAG CACGCAGAGC GAGGACTTCT CTAGGCGGCA CACGGTCCCG 
GACAAGCACG TCCCCATCGC GTTGCCCCGC CAGAACAGCA GCAGCCAGGC ACAGATCCGC 
AAAATGGACC AGGCCGGGTT AGGGGCCAGG GCCAAAGCGC CGCAGTCGCT GCCGCACAGC 
GCGTCCCTGC GCAGCACGGG CAGCATGTCG GCCGTGTCGG GGGCCGTGGT GACTGAGCCC 
GTCCAGAACG GCAGCCGCTC CCGGCAGCAG TCGTCCTCCT CCAGGGAGAG CCCTGTGCCG 
AAGGTGAGGG CCATTCAGAG GCAGCAAACG CAGCAGGTCC AGTCTCCTGT GGACTCAGCC 
ACAATGTCAC CAGTGGAAAG GACAGCAGCG TGGGTTCTCA ATAACGGGCA GTACGAGGAG 
GTCGAGGAGG ACACAGAGCA GAACCCAGAT GAAGCCAAGC ATGCTGAGAA GTACGAACAA 
GAGATAGCAA AGCTGAAGGA ACGCTTGAAA GTGTCGAGCC GCCGGCTGGA GGAGTACGAG 
CGGCGGCTCC TGGTGCAGGA GCAGCAAATG CAGAAGCTGC TGATGGAGTA CAAGTCCAGG 
CTGGAGGACA GCGAGGAGAG ACTGCGCAGG CAACAGGAGG AGAAGGACAG CCAGATGAAA 
AGCATTATCA GCAGGCTCAT GGCAGTTGAA GAGGAACTGA AGAAAGATCA TGCAGAGATG 
CAAGCTGTCA TCGATGCAAA GCAGAAAATT ATTGATGCAC AGGAGAAGCG GATCGTGTCC 
CTGGACTCTG CCAACACGCG GCTGATGAGT GCCCTGACGC AGGTGAAGGA GCGCTACAGC 
ATGCAAGTCC GCAATGGCAT CTCCCCCACC AACCCCACCA AGCTTTCCAT CACGGAGAAT 
GGTGAATTCA AAAACAGCAG CTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004943302.2
CDS	633..4919
Translation	MSAFKKYYSLSPRTFRRSLSEQDAGTAALKDYNWYHQMRSKKGSKQTALDREKALGAYRWQTHPRLPWRTSRRAHSCSLRGEGNDSSLVKSSASMDATSADDLHQDSTEMLFQARTRSPRVPFNRSLSEPVPHSANRNAKPFLQAVRSVDCEQHSYFVRGIFSTLSSGFSKIINRRGEQPPTEPLEEWKPAGSQIDPCPGNGLCSVCVTDVKGPPTHRLSCGQSPYTETTTWERKYCILTDSQLVLLNREKEVPTEGLQEPQDATKGRCLRRTVSVPSEGQFPEYPPDGAAKLEVPAERSPRRRSISGTSTSEKTNSMDAANTSPFKVSGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNADDRSRGLPKLKESRSHESLLSPGSAVEALDLGAEEKVFVKPLHSSILGQDFCFEVTYSSGSKCFSCSSAAERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYGLPALHSITVHIYKDVEKKKKKDKNNYVGLVNIPMATVTGRQFVEKWYPVSTPTPNKGKSGGPSIRIKSRYQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEEMACAMVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTLATKAIEEYLKLVGQKYLHDALGEFIKALYESDENCEVDPSKCSSSELTDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCLSRGKQDISERLISASLFLRFLCPAIMSPSLFSLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNMPGFEGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVAKLGPLPRILADITKSMTNPTPIQQQLRRFTEHSSSPNVSGSLSSGLQKIFEDPTDGDLHKLKSPTQENIDGYFRGKTLLLVQQASTQSMTYSDKDERESILPNGRSISLMDLQDPHTAHSDHASMMHDVPLRLAGSQLSITQVANIKQLWETQSTPQSAPQVRRPLHPALNQQGSLQPLSFQNPVYHLNNPPAPLPKASVDSSLENLSTASSQSRSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTVPDKHVPIALPRQNSSSQAQIRKMDQAGLGARAKAPQSLPHSASLRSTGSMSAVSGAVVTEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEVEEDTEQNPDEAKHAEKYEQEIAKLKERLKVSSRRLEEYERRLLVQEQQMQKLLMEYKSRLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQEKRIVSLDSANTRLMSALTQVKERYSMQVRNGISPTNPTKLSITENGEFKNSSC

Target ORF information:

RefSeq Version	XM_004943245.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004943245.2

ORF Insert Sequence:

ATGAGTGCTT TTAAAAAGTA TTACAGTCTC TCTCCCAGGA CCTTTAGGAG GTCTTTGTCG 
GAGCAAGATG CTGGCACCGC TGCCTTAAAG GACTATAATT GGTACCACCA AATGAGAAGC 
AAGAAGGGGA GCAAACAAAC AGCTTTGGAC CGAGAGAAAG CACTCGGAGC GTACAGGTGG 
CAAACACATC CCCGTCTTCC CTGGAGGACA AGCAGAAGAG CACACTCTTG CAGCCTCAGA 
GGGGAAGGTA ATGATTCGAG TCTTGTCAAA AGTTCTGCAA GCATGGATGC TACCAGCGCC 
GACGATCTCC ACCAAGACTC AACGGAGATG CTCTTCCAGG CAAGGACCAG ATCACCGCGG 
GTTCCCTTCA ACCGCTCCCT GTCTGAACCT GTGCCGCACT CTGCAAACAG GAATGCCAAG 
CCCTTTCTGC AGGCGGTGCG CTCTGTGGAT TGTGAGCAGC ACAGCTATTT TGTCCGTGGC 
ATCTTCTCAA CTCTCTCAAG TGGCTTTTCA AAGATAATAA ATCGAAGGGG TGAGCAGCCC 
CCCACTGAGC CACTGGAGGA GTGGAAACCA GCCGGTAGTC AAATTGACCC GTGCCCAGGT 
AATGGACTCT GTTCTGTTTG TGTTACAGAT GTAAAAGGAC CACCTACGCA CAGGCTGTCC 
TGCGGCCAGT CCCCCTACAC CGAGACGACA ACATGGGAGA GGAAGTACTG CATCCTGACT 
GACAGCCAGC TGGTGCTGCT CAACAGGGAG AAGGAGGTGC CCACCGAGGG GTTGCAGGAG 
CCGCAGGATG CAACCAAAGG CCGCTGTCTG CGCCGCACGG TGAGCGTGCC CTCCGAGGGC 
CAGTTCCCCG AGTACCCACC AGATGGGGCC GCCAAGCTGG AGGTCCCGGC AGAGAGATCT 
CCTCGCAGAC GGAGCATCTC TGGGACCAGC ACATCTGAGA AAACCAACTC CATGGATGCT 
GCAAATACCT CTCCTTTCAA AGTATCGGGC TTCTTCAGTA AACGTCTGAA AGGGTCCATC 
AAGAGGACAA AGAGTCAGTC AAAGCTGGAC AGGAACACAA GTTTCCGCCT CCCATCCCTC 
CGCAATGCTG ATGACAGGTC ACGTGGGCTG CCGAAGCTGA AGGAGTCCCG CTCTCATGAG 
TCCCTGCTGA GCCCCGGCAG CGCAGTGGAA GCCCTGGACC TTGGGGCAGA AGAAAAGGTC 
TTTGTAAAGC CACTGCACAG CAGCATCCTC GGACAGGACT TCTGCTTTGA GGTAACCTAC 
TCCAGCGGCA GTAAATGCTT CAGCTGTTCC TCTGCTGCAG AGAGGGATAA GTGGATGGAA 
AACCTACGCA GAACAGTGCA GCCGAATAAG GACAACTGCC GTCGAGCTGA AAATGTGCTC 
CGTCTCTGGA TCATTGAGGC AAAGGACCTG GCCCCCAAGA AGAAATACTT CTGTGAGCTG 
TGCCTTGATG ACACTCTCTT TGCCCGCACC ACCAGCAAAA CGAAAGCAGA TAACATTTTC 
TGGGGGGAGC ACTTTGAGTT CTACGGCCTC CCAGCTCTTC ACAGCATCAC GGTTCACATC 
TACAAGGATG TGGAGAAGAA AAAGAAAAAG GACAAAAACA ATTACGTCGG CCTGGTCAAC 
ATTCCCATGG CCACTGTAAC GGGGCGCCAG TTTGTGGAAA AATGGTACCC AGTCAGCACA 
CCTACACCCA ACAAAGGGAA ATCAGGAGGC CCTTCCATTC GCATCAAGTC GCGTTATCAG 
ACCATCACCA TCTTACCCAT GGAGCAATAC AAGGAGTTTG CAGAGTTTGT TACCAGCAAC 
TACACGATGC TGTGCTCTGT GCTGGAGCCA GTCATCAGCG TAAGGAATAA GGAAGAGATG 
GCTTGTGCAA TGGTGCACAT TCTTCAGAGC ACGGGGAGAG CCAAGGACTT CCTAACGGAT 
CTGGTGATGT CTGAGGTGGA TCGCTGCGGG GAACACGATG TCTTGATCTT CAGGGAGAAC 
ACACTTGCTA CCAAAGCTAT TGAGGAATAC CTGAAGTTGG TAGGGCAGAA GTATCTCCAT 
GATGCGCTAG GGGAGTTTAT CAAGGCTTTG TATGAGTCAG ATGAAAACTG TGAAGTGGAC 
CCCAGCAAAT GTTCATCCAG TGAATTAACA GACCATCAGA GCAACCTGAA AATGTGTTGT 
GAGCTGGCCT TCTGCAAGAT TATCAATTCC TACTGCGTGT TCCCTCGTGA GCTGAAGGAG 
GTGTTTGCAT CTTGGAAGCA GCAGTGCCTG AGCAGGGGCA AGCAGGACAT CAGCGAGCGG 
CTGATCAGTG CCTCCCTGTT CCTCCGCTTC CTCTGCCCCG CCATCATGTC CCCCAGCCTC 
TTCAGCCTCA TGCAGGAGTA TCCTGACGAC CGGACATCGC GCACGCTCAC ACTCATTGCT 
AAAGTCATTC AGAATCTCGC CAATTTTGCT AAGTTTGGTA ACAAGGAAGA GTACATGGCT 
TTCATGAACG ACTTTCTGGA ACATGAGTGG GGGGGAATGA AGCGATTTCT CCTGGAGATC 
TCTAATCCAG ATACCATCTC AAACATGCCT GGATTTGAGG GCTATATCGA CCTGGGCAGA 
GAGCTCTCAG TTTTGCATTC CCTCTTATGG GAGGTTGTGT CCCAACTTGA TAAGGGTGAA 
AATTCCTTCC TACAGGCGAC CGTGGCAAAA CTGGGACCTC TTCCTCGTAT TCTTGCCGAC 
ATCACCAAAT CAATGACCAA CCCTACGCCA ATACAGCAGC AGCTGAGGCG TTTCACTGAG 
CACAGCTCTA GTCCAAACGT CAGTGGCAGT CTCTCCTCAG GGCTTCAGAA GATATTCGAG 
GACCCCACTG ATGGTGACTT GCATAAACTG AAGTCTCCAA CCCAGGAAAA CATAGATGGC 
TATTTCAGGG GCAAGACCTT GCTGTTGGTT CAGCAGGCGT CCACCCAGAG CATGACTTAC 
TCAGATAAGG ACGAAAGAGA GAGCATCTTG CCCAACGGAC GTAGCATCTC CCTCATGGAC 
CTGCAGGATC CCCATACCGC TCACAGCGAC CACGCTTCCA TGATGCACGA CGTGCCTTTG 
CGCTTGGCCG GCAGCCAGCT CTCCATCACC CAGGTGGCCA ACATCAAGCA GCTGTGGGAG 
ACCCAGAGCA CGCCCCAAAG CGCTCCCCAG GTGAGGAGGC CGCTGCACCC GGCTTTGAAC 
CAGCAGGGCA GCCTCCAGCC TCTGTCATTC CAGAACCCGG TGTACCACCT CAACAACCCA 
CCTGCGCCGT TGCCCAAGGC CTCCGTGGAC TCCAGCCTGG AGAACCTGAG CACCGCCAGC 
TCCCAGAGCC GAAGCAACAG CGAAGACTTC AAGCTCAGCG GGCCCAGCAA CAGCAGCATG 
GAGGACTTCA CCAAGCGCAG CACGCAGAGC GAGGACTTCT CTAGGCGGCA CACGGTCCCG 
GACAAGCACG TCCCCATCGC GTTGCCCCGC CAGAACAGCA GCAGCCAGGC ACAGATCCGC 
AAAATGGACC AGGCCGGGTT AGGGGCCAGG GCCAAAGCGC CGCAGTCGCT GCCGCACAGC 
GCGTCCCTGC GCAGCACGGG CAGCATGTCG GCCGTGTCGG GGGCCGTGGT GACTGAGCCC 
GTCCAGAACG GCAGCCGCTC CCGGCAGCAG TCGTCCTCCT CCAGGGAGAG CCCTGTGCCG 
AAGGTGAGGG CCATTCAGAG GCAGCAAACG CAGCAGGTCC AGTCTCCTGT GGACTCAGCC 
ACAATGTCAC CAGTGGAAAG GACAGCAGCG TGGGTTCTCA ATAACGGGCA GTACGAGGAG 
GTCGAGGAGG ACACAGAGCA GAACCCAGAT GAAGCCAAGC ATGCTGAGAA GTACGAACAA 
GAGATAGCAA AGCTGAAGGA ACGCTTGAAA GTGTCGAGCC GCCGGCTGGA GGAGTACGAG 
CGGCGGCTCC TGGTGCAGGA GCAGCAAATG CAGAAGCTGC TGATGGAGTA CAAGTCCAGG 
CTGGAGGACA GCGAGGAGAG ACTGCGCAGG CAACAGGAGG AGAAGGACAG CCAGATGAAA 
AGCATTATCA GCAGGCTCAT GGCAGTTGAA GAGGAACTGA AGAAAGATCA TGCAGAGATG 
CAAGCTGTCA TCGATGCAAA GCAGAAAATT ATTGATGCAC AGGAGAAGCG GATCGTGTCC 
CTGGACTCTG CCAACACGCG GCTGATGAGT GCCCTGACGC AGGTGAAGGA GCGCTACAGC 
ATGCAAGTCC GCAATGGCAT CTCCCCCACC AACCCCACCA AGCTTTCCAT CACGGAGAAT 
GGTGAATTCA AAAACAGCAG CTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_015290511.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015145997.1 Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OGa39041
Clone ID Related Accession (Same CDS sequence)	XM_015290511.1
Accession Version	XM_015290511.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4275bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	ras GTPase-activating protein nGAP isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGCTT TTAAAAAGTA TTACAGTCTC TCTCCCAGGA CCTTTAGGAG GTCTTTGTCG 
GAGCAAGATG CTGGCACCGC TGCCTTAAAG GACTATAATT GGTACCACCA AATGAGAAGC 
AAGAAGGGGA GCAAACAAAC AGCTTTGGAC CGAGAGAAAG CACTCGGAGC GTACAGGTGG 
CAAACACATC CCCGTCTTCC CTGGAGGACA AGCAGAAGAG CACACTCTTG CAGCCTCAGA 
GGGGAAGGTA ATGATTCGAG TCTTGTCAAA AGTTCTGCAA GCATGGATGC TACCAGCGCC 
GACGATCTCC ACCAAGACTC AACGGAGATG CTCTTCCAGG CAAGGACCAG ATCACCGCGG 
GTTCCCTTCA ACCGCTCCCT GTCTGAACCT GTGCCGCACT CTGCAAACAG GAATGCCAAG 
CCCTTTCTGC AGGCGGTGCG CTCTGTGGAT TGTGAGCAGC ACAGCTATTT TGTCCGTGGC 
ATCTTCTCAA CTCTCTCAAG TGGCTTTTCA AAGATAATAA ATCGAAGGGG TGAGCAGCCC 
CCCACTGAGC CACTGGAGGA GTGGAAACCA GCCGGTAGTC AAATTGACCC GTGCCCAGGT 
AATGGACTCT GTTCTGTTTG TGTTACAGAT GTAAAAGGAC CACCTACGCA CAGGCTGTCC 
TGCGGCCAGT CCCCCTACAC CGAGACGACA ACATGGGAGA GGAAGTACTG CATCCTGACT 
GACAGCCAGC TGGTGCTGCT CAACAGGGAG AAGGAGGTGC CCACCGAGGG GTTGCAGGAG 
CCGCAGGATG CAACCAAAGG CCGCTGTCTG CGCCGCACGG TGAGCGTGCC CTCCGAGGGC 
CAGTTCCCCG AGTACCCACC AGATGGGGCC GCCAAGCTGG AGGTCCCGGC AGAGAGATCT 
CCTCGCAGAC GGAGCATCTC TGGGACCAGC ACATCTGAGA AAACCAACTC CATGGATGCT 
GCAAATACCT CTCCTTTCAA AGTATCGGGC TTCTTCAGTA AACGTCTGAA AGGGTCCATC 
AAGAGGACAA AGAGTCAGTC AAAGCTGGAC AGGAACACAA GTTTCCGCCT CCCATCCCTC 
CGCAATGCTG ATGACAGGTC ACGTGGGCTG CCGAAGCTGA AGGAGTCCCG CTCTCATGAG 
TCCCTGCTGA GCCCCGGCAG CGCAGTGGAA GCCCTGGACC TTGGGGCAGA AGAAAAGGTC 
TTTGTAAAGC CACTGCACAG CAGCATCCTC GGACAGGACT TCTGCTTTGA GGTAACCTAC 
TCCAGCGGCA GTAAATGCTT CAGCTGTTCC TCTGCTGCAG AGAGGGATAA GTGGATGGAA 
AACCTACGCA GAACAGTGCA GCCGAATAAG GACAACTGCC GTCGAGCTGA AAATGTGCTC 
CGTCTCTGGA TCATTGAGGC AAAGGACCTG GCCCCCAAGA AGAAATACTT CTGTGAGCTG 
TGCCTTGATG ACACTCTCTT TGCCCGCACC ACCAGCAAAA CGAAAGCAGA TAACATTTTC 
TGGGGGGAGC ACTTTGAGTT CTACGGCCTC CCAGCTCTTC ACAGCATCAC GGTTCACATC 
TACAAGGATG TGGAGAAGAA AAAGAAAAAG GACAAAAACA ATTACGTCGG CCTGGTCAAC 
ATTCCCATGG CCACTGTAAC GGGGCGCCAG TTTGTGGAAA AATGGTACCC AGTCAGCACA 
CCTACACCCA ACAAAGGGAA ATCAGGAGGC CCTTCCATTC GCATCAAGTC GCGTTATCAG 
ACCATCACCA TCTTACCCAT GGAGCAATAC AAGGAGTTTG CAGAGTTTGT TACCAGCAAC 
TACACGATGC TGTGCTCTGT GCTGGAGCCA GTCATCAGCG TAAGGAATAA GGAAGAGATG 
GCTTGTGCAA TGGTGCACAT TCTTCAGAGC ACGGGGAGAG CCAAGGACTT CCTAACGGAT 
CTGGTGATGT CTGAGGTGGA TCGCTGCGGG GAACACGATG TCTTGATCTT CAGGGAGAAC 
ACACTTGCTA CCAAAGCTAT TGAGGAATAC CTGAAGTTGG TAGGGCAGAA GTATCTCCAT 
GATGCGCTAG GGGAGTTTAT CAAGGCTTTG TATGAGTCAG ATGAAAACTG TGAAGTGGAC 
CCCAGCAAAT GTTCATCCAG TGAATTAACA GACCATCAGA GCAACCTGAA AATGTGTTGT 
GAGCTGGCCT TCTGCAAGAT TATCAATTCC TACTGCGTGT TCCCTCGTGA GCTGAAGGAG 
GTGTTTGCAT CTTGGAAGCA GCAGTGCCTG AGCAGGGGCA AGCAGGACAT CAGCGAGCGG 
CTGATCAGTG CCTCCCTGTT CCTCCGCTTC CTCTGCCCCG CCATCATGTC CCCCAGCCTC 
TTCAGCCTCA TGCAGGAGTA TCCTGACGAC CGGACATCGC GCACGCTCAC ACTCATTGCT 
AAAGTCATTC AGAATCTCGC CAATTTTGCT AAGTTTGGTA ACAAGGAAGA GTACATGGCT 
TTCATGAACG ACTTTCTGGA ACATGAGTGG GGGGGAATGA AGCGATTTCT CCTGGAGATC 
TCTAATCCAG ATACCATCTC AAACATGCCT GGATTTGAGG GCTATATCGA CCTGGGCAGA 
GAGCTCTCAG TTTTGCATTC CCTCTTATGG GAGGTTGTGT CCCAACTTGA TAAGGCGACC 
GTGGCAAAAC TGGGACCTCT TCCTCGTATT CTTGCCGACA TCACCAAATC AATGACCAAC 
CCTACGCCAA TACAGCAGCA GCTGAGGCGT TTCACTGAGC ACAGCTCTAG TCCAAACGTC 
AGTGGCAGTC TCTCCTCAGG GCTTCAGAAG ATATTCGAGG ACCCCACTGA TGGTGACTTG 
CATAAACTGA AGTCTCCAAC CCAGGAAAAC ATAGATGGCT ATTTCAGGGG CAAGACCTTG 
CTGTTGGTTC AGCAGGCGTC CACCCAGAGC ATGACTTACT CAGATAAGGA CGAAAGAGAG 
AGCATCTTGC CCAACGGACG TAGCATCTCC CTCATGGACC TGCAGGATCC CCATACCGCT 
CACAGCGACC ACGCTTCCAT GATGCACGAC GTGCCTTTGC GCTTGGCCGG CAGCCAGCTC 
TCCATCACCC AGGTGGCCAA CATCAAGCAG CTGTGGGAGA CCCAGAGCAC GCCCCAAAGC 
GCTCCCCAGG TGAGGAGGCC GCTGCACCCG GCTTTGAACC AGCAGGGCAG CCTCCAGCCT 
CTGTCATTCC AGAACCCGGT GTACCACCTC AACAACCCAC CTGCGCCGTT GCCCAAGGCC 
TCCGTGGACT CCAGCCTGGA GAACCTGAGC ACCGCCAGCT CCCAGAGCCG AAGCAACAGC 
GAAGACTTCA AGCTCAGCGG GCCCAGCAAC AGCAGCATGG AGGACTTCAC CAAGCGCAGC 
ACGCAGAGCG AGGACTTCTC TAGGCGGCAC ACGGTCCCGG ACAAGCACGT CCCCATCGCG 
TTGCCCCGCC AGAACAGCAG CAGCCAGGCA CAGATCCGCA AAATGGACCA GGCCGGGTTA 
GGGGCCAGGG CCAAAGCGCC GCAGTCGCTG CCGCACAGCG CGTCCCTGCG CAGCACGGGC 
AGCATGTCGG CCGTGTCGGG GGCCGTGGTG ACTGAGCCCG TCCAGAACGG CAGCCGCTCC 
CGGCAGCAGT CGTCCTCCTC CAGGGAGAGC CCTGTGCCGA AGGTGAGGGC CATTCAGAGG 
CAGCAAACGC AGCAGGTCCA GTCTCCTGTG GACTCAGCCA CAATGTCACC AGTGGAAAGG 
ACAGCAGCGT GGGTTCTCAA TAACGGGCAG TACGAGGAGG TCGAGGAGGA CACAGAGCAG 
AACCCAGATG AAGCCAAGCA TGCTGAGAAG TACGAACAAG AGATAGCAAA GCTGAAGGAA 
CGCTTGAAAG TGTCGAGCCG CCGGCTGGAG GAGTACGAGC GGCGGCTCCT GGTGCAGGAG 
CAGCAAATGC AGAAGCTGCT GATGGAGTAC AAGTCCAGGC TGGAGGACAG CGAGGAGAGA 
CTGCGCAGGC AACAGGAGGA GAAGGACAGC CAGATGAAAA GCATTATCAG CAGGCTCATG 
GCAGTTGAAG AGGAACTGAA GAAAGATCAT GCAGAGATGC AAGCTGTCAT CGATGCAAAG 
CAGAAAATTA TTGATGCACA GGTCATTAAT GGGAATGAGA TAATTCAAAC AGGAGAAGCG 
GATCGTGTCC CTGGACTCTG CCAACACGCG GCTGATGAGT GCCCTGACGC AGGTGAAGGA 
GCGCTACAGC ATGCAAGTCC GCAATGGCAT CTCCCCCACC AACCCCACCA AGCTTTCCAT 
CACGGAGAAT GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015145997.1
CDS	638..4912
Translation	MSAFKKYYSLSPRTFRRSLSEQDAGTAALKDYNWYHQMRSKKGSKQTALDREKALGAYRWQTHPRLPWRTSRRAHSCSLRGEGNDSSLVKSSASMDATSADDLHQDSTEMLFQARTRSPRVPFNRSLSEPVPHSANRNAKPFLQAVRSVDCEQHSYFVRGIFSTLSSGFSKIINRRGEQPPTEPLEEWKPAGSQIDPCPGNGLCSVCVTDVKGPPTHRLSCGQSPYTETTTWERKYCILTDSQLVLLNREKEVPTEGLQEPQDATKGRCLRRTVSVPSEGQFPEYPPDGAAKLEVPAERSPRRRSISGTSTSEKTNSMDAANTSPFKVSGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNADDRSRGLPKLKESRSHESLLSPGSAVEALDLGAEEKVFVKPLHSSILGQDFCFEVTYSSGSKCFSCSSAAERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYGLPALHSITVHIYKDVEKKKKKDKNNYVGLVNIPMATVTGRQFVEKWYPVSTPTPNKGKSGGPSIRIKSRYQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEEMACAMVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTLATKAIEEYLKLVGQKYLHDALGEFIKALYESDENCEVDPSKCSSSELTDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCLSRGKQDISERLISASLFLRFLCPAIMSPSLFSLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNMPGFEGYIDLGRELSVLHSLLWEVVSQLDKATVAKLGPLPRILADITKSMTNPTPIQQQLRRFTEHSSSPNVSGSLSSGLQKIFEDPTDGDLHKLKSPTQENIDGYFRGKTLLLVQQASTQSMTYSDKDERESILPNGRSISLMDLQDPHTAHSDHASMMHDVPLRLAGSQLSITQVANIKQLWETQSTPQSAPQVRRPLHPALNQQGSLQPLSFQNPVYHLNNPPAPLPKASVDSSLENLSTASSQSRSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTVPDKHVPIALPRQNSSSQAQIRKMDQAGLGARAKAPQSLPHSASLRSTGSMSAVSGAVVTEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEVEEDTEQNPDEAKHAEKYEQEIAKLKERLKVSSRRLEEYERRLLVQEQQMQKLLMEYKSRLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQVINGNEIIQTGEADRVPGLCQHAADECPDAGEGALQHASPQWHLPHQPHQAFHHGEW

Target ORF information:

RefSeq Version	XM_015290511.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015290511.1

ORF Insert Sequence:

ATGAGTGCTT TTAAAAAGTA TTACAGTCTC TCTCCCAGGA CCTTTAGGAG GTCTTTGTCG 
GAGCAAGATG CTGGCACCGC TGCCTTAAAG GACTATAATT GGTACCACCA AATGAGAAGC 
AAGAAGGGGA GCAAACAAAC AGCTTTGGAC CGAGAGAAAG CACTCGGAGC GTACAGGTGG 
CAAACACATC CCCGTCTTCC CTGGAGGACA AGCAGAAGAG CACACTCTTG CAGCCTCAGA 
GGGGAAGGTA ATGATTCGAG TCTTGTCAAA AGTTCTGCAA GCATGGATGC TACCAGCGCC 
GACGATCTCC ACCAAGACTC AACGGAGATG CTCTTCCAGG CAAGGACCAG ATCACCGCGG 
GTTCCCTTCA ACCGCTCCCT GTCTGAACCT GTGCCGCACT CTGCAAACAG GAATGCCAAG 
CCCTTTCTGC AGGCGGTGCG CTCTGTGGAT TGTGAGCAGC ACAGCTATTT TGTCCGTGGC 
ATCTTCTCAA CTCTCTCAAG TGGCTTTTCA AAGATAATAA ATCGAAGGGG TGAGCAGCCC 
CCCACTGAGC CACTGGAGGA GTGGAAACCA GCCGGTAGTC AAATTGACCC GTGCCCAGGT 
AATGGACTCT GTTCTGTTTG TGTTACAGAT GTAAAAGGAC CACCTACGCA CAGGCTGTCC 
TGCGGCCAGT CCCCCTACAC CGAGACGACA ACATGGGAGA GGAAGTACTG CATCCTGACT 
GACAGCCAGC TGGTGCTGCT CAACAGGGAG AAGGAGGTGC CCACCGAGGG GTTGCAGGAG 
CCGCAGGATG CAACCAAAGG CCGCTGTCTG CGCCGCACGG TGAGCGTGCC CTCCGAGGGC 
CAGTTCCCCG AGTACCCACC AGATGGGGCC GCCAAGCTGG AGGTCCCGGC AGAGAGATCT 
CCTCGCAGAC GGAGCATCTC TGGGACCAGC ACATCTGAGA AAACCAACTC CATGGATGCT 
GCAAATACCT CTCCTTTCAA AGTATCGGGC TTCTTCAGTA AACGTCTGAA AGGGTCCATC 
AAGAGGACAA AGAGTCAGTC AAAGCTGGAC AGGAACACAA GTTTCCGCCT CCCATCCCTC 
CGCAATGCTG ATGACAGGTC ACGTGGGCTG CCGAAGCTGA AGGAGTCCCG CTCTCATGAG 
TCCCTGCTGA GCCCCGGCAG CGCAGTGGAA GCCCTGGACC TTGGGGCAGA AGAAAAGGTC 
TTTGTAAAGC CACTGCACAG CAGCATCCTC GGACAGGACT TCTGCTTTGA GGTAACCTAC 
TCCAGCGGCA GTAAATGCTT CAGCTGTTCC TCTGCTGCAG AGAGGGATAA GTGGATGGAA 
AACCTACGCA GAACAGTGCA GCCGAATAAG GACAACTGCC GTCGAGCTGA AAATGTGCTC 
CGTCTCTGGA TCATTGAGGC AAAGGACCTG GCCCCCAAGA AGAAATACTT CTGTGAGCTG 
TGCCTTGATG ACACTCTCTT TGCCCGCACC ACCAGCAAAA CGAAAGCAGA TAACATTTTC 
TGGGGGGAGC ACTTTGAGTT CTACGGCCTC CCAGCTCTTC ACAGCATCAC GGTTCACATC 
TACAAGGATG TGGAGAAGAA AAAGAAAAAG GACAAAAACA ATTACGTCGG CCTGGTCAAC 
ATTCCCATGG CCACTGTAAC GGGGCGCCAG TTTGTGGAAA AATGGTACCC AGTCAGCACA 
CCTACACCCA ACAAAGGGAA ATCAGGAGGC CCTTCCATTC GCATCAAGTC GCGTTATCAG 
ACCATCACCA TCTTACCCAT GGAGCAATAC AAGGAGTTTG CAGAGTTTGT TACCAGCAAC 
TACACGATGC TGTGCTCTGT GCTGGAGCCA GTCATCAGCG TAAGGAATAA GGAAGAGATG 
GCTTGTGCAA TGGTGCACAT TCTTCAGAGC ACGGGGAGAG CCAAGGACTT CCTAACGGAT 
CTGGTGATGT CTGAGGTGGA TCGCTGCGGG GAACACGATG TCTTGATCTT CAGGGAGAAC 
ACACTTGCTA CCAAAGCTAT TGAGGAATAC CTGAAGTTGG TAGGGCAGAA GTATCTCCAT 
GATGCGCTAG GGGAGTTTAT CAAGGCTTTG TATGAGTCAG ATGAAAACTG TGAAGTGGAC 
CCCAGCAAAT GTTCATCCAG TGAATTAACA GACCATCAGA GCAACCTGAA AATGTGTTGT 
GAGCTGGCCT TCTGCAAGAT TATCAATTCC TACTGCGTGT TCCCTCGTGA GCTGAAGGAG 
GTGTTTGCAT CTTGGAAGCA GCAGTGCCTG AGCAGGGGCA AGCAGGACAT CAGCGAGCGG 
CTGATCAGTG CCTCCCTGTT CCTCCGCTTC CTCTGCCCCG CCATCATGTC CCCCAGCCTC 
TTCAGCCTCA TGCAGGAGTA TCCTGACGAC CGGACATCGC GCACGCTCAC ACTCATTGCT 
AAAGTCATTC AGAATCTCGC CAATTTTGCT AAGTTTGGTA ACAAGGAAGA GTACATGGCT 
TTCATGAACG ACTTTCTGGA ACATGAGTGG GGGGGAATGA AGCGATTTCT CCTGGAGATC 
TCTAATCCAG ATACCATCTC AAACATGCCT GGATTTGAGG GCTATATCGA CCTGGGCAGA 
GAGCTCTCAG TTTTGCATTC CCTCTTATGG GAGGTTGTGT CCCAACTTGA TAAGGCGACC 
GTGGCAAAAC TGGGACCTCT TCCTCGTATT CTTGCCGACA TCACCAAATC AATGACCAAC 
CCTACGCCAA TACAGCAGCA GCTGAGGCGT TTCACTGAGC ACAGCTCTAG TCCAAACGTC 
AGTGGCAGTC TCTCCTCAGG GCTTCAGAAG ATATTCGAGG ACCCCACTGA TGGTGACTTG 
CATAAACTGA AGTCTCCAAC CCAGGAAAAC ATAGATGGCT ATTTCAGGGG CAAGACCTTG 
CTGTTGGTTC AGCAGGCGTC CACCCAGAGC ATGACTTACT CAGATAAGGA CGAAAGAGAG 
AGCATCTTGC CCAACGGACG TAGCATCTCC CTCATGGACC TGCAGGATCC CCATACCGCT 
CACAGCGACC ACGCTTCCAT GATGCACGAC GTGCCTTTGC GCTTGGCCGG CAGCCAGCTC 
TCCATCACCC AGGTGGCCAA CATCAAGCAG CTGTGGGAGA CCCAGAGCAC GCCCCAAAGC 
GCTCCCCAGG TGAGGAGGCC GCTGCACCCG GCTTTGAACC AGCAGGGCAG CCTCCAGCCT 
CTGTCATTCC AGAACCCGGT GTACCACCTC AACAACCCAC CTGCGCCGTT GCCCAAGGCC 
TCCGTGGACT CCAGCCTGGA GAACCTGAGC ACCGCCAGCT CCCAGAGCCG AAGCAACAGC 
GAAGACTTCA AGCTCAGCGG GCCCAGCAAC AGCAGCATGG AGGACTTCAC CAAGCGCAGC 
ACGCAGAGCG AGGACTTCTC TAGGCGGCAC ACGGTCCCGG ACAAGCACGT CCCCATCGCG 
TTGCCCCGCC AGAACAGCAG CAGCCAGGCA CAGATCCGCA AAATGGACCA GGCCGGGTTA 
GGGGCCAGGG CCAAAGCGCC GCAGTCGCTG CCGCACAGCG CGTCCCTGCG CAGCACGGGC 
AGCATGTCGG CCGTGTCGGG GGCCGTGGTG ACTGAGCCCG TCCAGAACGG CAGCCGCTCC 
CGGCAGCAGT CGTCCTCCTC CAGGGAGAGC CCTGTGCCGA AGGTGAGGGC CATTCAGAGG 
CAGCAAACGC AGCAGGTCCA GTCTCCTGTG GACTCAGCCA CAATGTCACC AGTGGAAAGG 
ACAGCAGCGT GGGTTCTCAA TAACGGGCAG TACGAGGAGG TCGAGGAGGA CACAGAGCAG 
AACCCAGATG AAGCCAAGCA TGCTGAGAAG TACGAACAAG AGATAGCAAA GCTGAAGGAA 
CGCTTGAAAG TGTCGAGCCG CCGGCTGGAG GAGTACGAGC GGCGGCTCCT GGTGCAGGAG 
CAGCAAATGC AGAAGCTGCT GATGGAGTAC AAGTCCAGGC TGGAGGACAG CGAGGAGAGA 
CTGCGCAGGC AACAGGAGGA GAAGGACAGC CAGATGAAAA GCATTATCAG CAGGCTCATG 
GCAGTTGAAG AGGAACTGAA GAAAGATCAT GCAGAGATGC AAGCTGTCAT CGATGCAAAG 
CAGAAAATTA TTGATGCACA GGTCATTAAT GGGAATGAGA TAATTCAAAC AGGAGAAGCG 
GATCGTGTCC CTGGACTCTG CCAACACGCG GCTGATGAGT GCCCTGACGC AGGTGAAGGA 
GCGCTACAGC ATGCAAGTCC GCAATGGCAT CTCCCCCACC AACCCCACCA AGCTTTCCAT 
CACGGAGAAT GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_004943246.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004943303.2 Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X4, mRNA.

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CloneID	OGa39042
Clone ID Related Accession (Same CDS sequence)	XM_004943246.2
Accession Version	XM_004943246.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4266bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	ras GTPase-activating protein nGAP isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004943246.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGCTT TTAAAAAGTA TTACAGTCTC TCTCCCAGGA CCTTTAGGAG GTCTTTGTCG 
GAGCAAGATG CTGGCACCGC TGCCTTAAAG GACTATAATT GGTACCACCA AATGAGAAGC 
AAGAAGGGGA GCAAACAAAC AGCTTTGGAC CGAGAGAAAG CACTCGGAGC GTACAGGTGG 
CAAACACATC CCCGTCTTCC CTGGAGGACA AGCAGAAGAG CACACTCTTG CAGCCTCAGA 
GGGGAAGGTA ATGATTCGAG TCTTGTCAAA AGTTCTGCAA GCATGGATGC TACCAGCGCC 
GACGATCTCC ACCAAGACTC AACGGAGATG CTCTTCCAGG CAAGGACCAG ATCACCGCGG 
GTTCCCTTCA ACCGCTCCCT GTCTGAACCT GTGCCGCACT CTGCAAACAG GAATGCCAAG 
CCCTTTCTGC AGGCGGTGCG CTCTGTGGAT TGTGAGCAGC ACAGCTATTT TGTCCGTGGC 
ATCTTCTCAA CTCTCTCAAG TGGCTTTTCA AAGATAATAA ATCGAAGGGG TGAGCAGCCC 
CCCACTGAGC CACTGGAGGA GTGGAAACCA GCCGGTAGTC AAATTGACCC GTGCCCAGGT 
AATGGACTCT GTTCTGTTTG TGTTACAGAT GTAAAAGGAC CACCTACGCA CAGGCTGTCC 
TGCGGCCAGT CCCCCTACAC CGAGACGACA ACATGGGAGA GGAAGTACTG CATCCTGACT 
GACAGCCAGC TGGTGCTGCT CAACAGGGAG AAGGAGGTGC CCACCGAGGG GTTGCAGGAG 
CCGCAGGATG CAACCAAAGG CCGCTGTCTG CGCCGCACGG TGAGCGTGCC CTCCGAGGGC 
CAGTTCCCCG AGTACCCACC AGATGGGGCC GCCAAGCTGG AGGTCCCGGC AGAGAGATCT 
CCTCGCAGAC GGAGCATCTC TGGGACCAGC ACATCTGAGA AAACCAACTC CATGGATGCT 
GCAAATACCT CTCCTTTCAA AGTATCGGGC TTCTTCAGTA AACGTCTGAA AGGGTCCATC 
AAGAGGACAA AGAGTCAGTC AAAGCTGGAC AGGAACACAA GTTTCCGCCT CCCATCCCTC 
CGCAATGCTG ATGACAGGTC ACGTGGGCTG CCGAAGCTGA AGGAGTCCCG CTCTCATGAG 
TCCCTGCTGA GCCCCGGCAG CGCAGTGGAA GCCCTGGACC TTGGGGCAGA AGAAAAGGTC 
TTTGTAAAGC CACTGCACAG CAGCATCCTC GGACAGGACT TCTGCTTTGA GGTAACCTAC 
TCCAGCGGCA GTAAATGCTT CAGCTGTTCC TCTGCTGCAG AGAGGGATAA GTGGATGGAA 
AACCTACGCA GAACAGTGCA GCCGAATAAG GACAACTGCC GTCGAGCTGA AAATGTGCTC 
CGTCTCTGGA TCATTGAGGC AAAGGACCTG GCCCCCAAGA AGAAATACTT CTGTGAGCTG 
TGCCTTGATG ACACTCTCTT TGCCCGCACC ACCAGCAAAA CGAAAGCAGA TAACATTTTC 
TGGGGGGAGC ACTTTGAGTT CTACGGCCTC CCAGCTCTTC ACAGCATCAC GGTTCACATC 
TACAAGGATG TGGAGAAGAA AAAGAAAAAG GACAAAAACA ATTACGTCGG CCTGGTCAAC 
ATTCCCATGG CCACTGTAAC GGGGCGCCAG TTTGTGGAAA AATGGTACCC AGTCAGCACA 
CCTACACCCA ACAAAGGGAA ATCAGGAGGC CCTTCCATTC GCATCAAGTC GCGTTATCAG 
ACCATCACCA TCTTACCCAT GGAGCAATAC AAGGAGTTTG CAGAGTTTGT TACCAGCAAC 
TACACGATGC TGTGCTCTGT GCTGGAGCCA GTCATCAGCG TAAGGAATAA GGAAGAGATG 
GCTTGTGCAA TGGTGCACAT TCTTCAGAGC ACGGGGAGAG CCAAGGACTT CCTAACGGAT 
CTGGTGATGT CTGAGGTGGA TCGCTGCGGG GAACACGATG TCTTGATCTT CAGGGAGAAC 
ACACTTGCTA CCAAAGCTAT TGAGGAATAC CTGAAGTTGG TAGGGCAGAA GTATCTCCAT 
GATGCGCTAG GGGAGTTTAT CAAGGCTTTG TATGAGTCAG ATGAAAACTG TGAAGTGGAC 
CCCAGCAAAT GTTCATCCAG TGAATTAACA GACCATCAGA GCAACCTGAA AATGTGTTGT 
GAGCTGGCCT TCTGCAAGAT TATCAATTCC TACTGCGTGT TCCCTCGTGA GCTGAAGGAG 
GTGTTTGCAT CTTGGAAGCA GCAGTGCCTG AGCAGGGGCA AGCAGGACAT CAGCGAGCGG 
CTGATCAGTG CCTCCCTGTT CCTCCGCTTC CTCTGCCCCG CCATCATGTC CCCCAGCCTC 
TTCAGCCTCA TGCAGGAGTA TCCTGACGAC CGGACATCGC GCACGCTCAC ACTCATTGCT 
AAAGTCATTC AGAATCTCGC CAATTTTGCT AAGTTTGGTA ACAAGGAAGA GTACATGGCT 
TTCATGAACG ACTTTCTGGA ACATGAGTGG GGGGGAATGA AGCGATTTCT CCTGGAGATC 
TCTAATCCAG ATACCATCTC AAACATGCCT GGATTTGAGG GCTATATCGA CCTGGGCAGA 
GAGCTCTCAG TTTTGCATTC CCTCTTATGG GAGGTTGTGT CCCAACTTGA TAAGGCGACC 
GTGGCAAAAC TGGGACCTCT TCCTCGTATT CTTGCCGACA TCACCAAATC AATGACCAAC 
CCTACGCCAA TACAGCAGCA GCTGAGGCGT TTCACTGAGC ACAGCTCTAG TCCAAACGTC 
AGTGGCAGTC TCTCCTCAGG GCTTCAGAAG ATATTCGAGG ACCCCACTGA TGGTGACTTG 
CATAAACTGA AGTCTCCAAC CCAGGAAAAC ATAGATGGCT ATTTCAGGGG CAAGACCTTG 
CTGTTGGTTC AGCAGGCGTC CACCCAGAGC ATGACTTACT CAGATAAGGA CGAAAGAGAG 
AGCATCTTGC CCAACGGACG TAGCATCTCC CTCATGGACC TGCAGGATCC CCATACCGCT 
CACAGCGACC ACGCTTCCAT GATGCACGAC GTGCCTTTGC GCTTGGCCGG CAGCCAGCTC 
TCCATCACCC AGGTGGCCAA CATCAAGCAG CTGTGGGAGA CCCAGAGCAC GCCCCAAAGC 
GCTCCCCAGG TGAGGAGGCC GCTGCACCCG GCTTTGAACC AGCAGGGCAG CCTCCAGCCT 
CTGTCATTCC AGAACCCGGT GTACCACCTC AACAACCCAC CTGCGCCGTT GCCCAAGGCC 
TCCGTGGACT CCAGCCTGGA GAACCTGAGC ACCGCCAGCT CCCAGAGCCG AAGCAACAGC 
GAAGACTTCA AGCTCAGCGG GCCCAGCAAC AGCAGCATGG AGGACTTCAC CAAGCGCAGC 
ACGCAGAGCG AGGACTTCTC TAGGCGGCAC ACGGTCCCGG ACAAGCACGT CCCCATCGCG 
TTGCCCCGCC AGAACAGCAG CAGCCAGGCA CAGATCCGCA AAATGGACCA GGCCGGGTTA 
GGGGCCAGGG CCAAAGCGCC GCAGTCGCTG CCGCACAGCG CGTCCCTGCG CAGCACGGGC 
AGCATGTCGG CCGTGTCGGG GGCCGTGGTG ACTGAGCCCG TCCAGAACGG CAGCCGCTCC 
CGGCAGCAGT CGTCCTCCTC CAGGGAGAGC CCTGTGCCGA AGGTGAGGGC CATTCAGAGG 
CAGCAAACGC AGCAGGTCCA GTCTCCTGTG GACTCAGCCA CAATGTCACC AGTGGAAAGG 
ACAGCAGCGT GGGTTCTCAA TAACGGGCAG TACGAGGAGG TCGAGGAGGA CACAGAGCAG 
AACCCAGATG AAGCCAAGCA TGCTGAGAAG TACGAACAAG AGATAGCAAA GCTGAAGGAA 
CGCTTGAAAG TGTCGAGCCG CCGGCTGGAG GAGTACGAGC GGCGGCTCCT GGTGCAGGAG 
CAGCAAATGC AGAAGCTGCT GATGGAGTAC AAGTCCAGGC TGGAGGACAG CGAGGAGAGA 
CTGCGCAGGC AACAGGAGGA GAAGGACAGC CAGATGAAAA GCATTATCAG CAGGCTCATG 
GCAGTTGAAG AGGAACTGAA GAAAGATCAT GCAGAGATGC AAGCTGTCAT CGATGCAAAG 
CAGAAAATTA TTGATGCACA GGAGAAGCGG ATCGTGTCCC TGGACTCTGC CAACACGCGG 
CTGATGAGTG CCCTGACGCA GGTGAAGGAG CGCTACAGCA TGCAAGTCCG CAATGGCATC 
TCCCCCACCA ACCCCACCAA GCTTTCCATC ACGGAGAATG GTGAATTCAA AAACAGCAGC 
TGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004943303.2
CDS	632..4897
Translation	MSAFKKYYSLSPRTFRRSLSEQDAGTAALKDYNWYHQMRSKKGSKQTALDREKALGAYRWQTHPRLPWRTSRRAHSCSLRGEGNDSSLVKSSASMDATSADDLHQDSTEMLFQARTRSPRVPFNRSLSEPVPHSANRNAKPFLQAVRSVDCEQHSYFVRGIFSTLSSGFSKIINRRGEQPPTEPLEEWKPAGSQIDPCPGNGLCSVCVTDVKGPPTHRLSCGQSPYTETTTWERKYCILTDSQLVLLNREKEVPTEGLQEPQDATKGRCLRRTVSVPSEGQFPEYPPDGAAKLEVPAERSPRRRSISGTSTSEKTNSMDAANTSPFKVSGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNADDRSRGLPKLKESRSHESLLSPGSAVEALDLGAEEKVFVKPLHSSILGQDFCFEVTYSSGSKCFSCSSAAERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYGLPALHSITVHIYKDVEKKKKKDKNNYVGLVNIPMATVTGRQFVEKWYPVSTPTPNKGKSGGPSIRIKSRYQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEEMACAMVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTLATKAIEEYLKLVGQKYLHDALGEFIKALYESDENCEVDPSKCSSSELTDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCLSRGKQDISERLISASLFLRFLCPAIMSPSLFSLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNMPGFEGYIDLGRELSVLHSLLWEVVSQLDKATVAKLGPLPRILADITKSMTNPTPIQQQLRRFTEHSSSPNVSGSLSSGLQKIFEDPTDGDLHKLKSPTQENIDGYFRGKTLLLVQQASTQSMTYSDKDERESILPNGRSISLMDLQDPHTAHSDHASMMHDVPLRLAGSQLSITQVANIKQLWETQSTPQSAPQVRRPLHPALNQQGSLQPLSFQNPVYHLNNPPAPLPKASVDSSLENLSTASSQSRSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTVPDKHVPIALPRQNSSSQAQIRKMDQAGLGARAKAPQSLPHSASLRSTGSMSAVSGAVVTEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEVEEDTEQNPDEAKHAEKYEQEIAKLKERLKVSSRRLEEYERRLLVQEQQMQKLLMEYKSRLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQEKRIVSLDSANTRLMSALTQVKERYSMQVRNGISPTNPTKLSITENGEFKNSSC

Target ORF information:

RefSeq Version	XM_004943246.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004943246.2

ORF Insert Sequence:

ATGAGTGCTT TTAAAAAGTA TTACAGTCTC TCTCCCAGGA CCTTTAGGAG GTCTTTGTCG 
GAGCAAGATG CTGGCACCGC TGCCTTAAAG GACTATAATT GGTACCACCA AATGAGAAGC 
AAGAAGGGGA GCAAACAAAC AGCTTTGGAC CGAGAGAAAG CACTCGGAGC GTACAGGTGG 
CAAACACATC CCCGTCTTCC CTGGAGGACA AGCAGAAGAG CACACTCTTG CAGCCTCAGA 
GGGGAAGGTA ATGATTCGAG TCTTGTCAAA AGTTCTGCAA GCATGGATGC TACCAGCGCC 
GACGATCTCC ACCAAGACTC AACGGAGATG CTCTTCCAGG CAAGGACCAG ATCACCGCGG 
GTTCCCTTCA ACCGCTCCCT GTCTGAACCT GTGCCGCACT CTGCAAACAG GAATGCCAAG 
CCCTTTCTGC AGGCGGTGCG CTCTGTGGAT TGTGAGCAGC ACAGCTATTT TGTCCGTGGC 
ATCTTCTCAA CTCTCTCAAG TGGCTTTTCA AAGATAATAA ATCGAAGGGG TGAGCAGCCC 
CCCACTGAGC CACTGGAGGA GTGGAAACCA GCCGGTAGTC AAATTGACCC GTGCCCAGGT 
AATGGACTCT GTTCTGTTTG TGTTACAGAT GTAAAAGGAC CACCTACGCA CAGGCTGTCC 
TGCGGCCAGT CCCCCTACAC CGAGACGACA ACATGGGAGA GGAAGTACTG CATCCTGACT 
GACAGCCAGC TGGTGCTGCT CAACAGGGAG AAGGAGGTGC CCACCGAGGG GTTGCAGGAG 
CCGCAGGATG CAACCAAAGG CCGCTGTCTG CGCCGCACGG TGAGCGTGCC CTCCGAGGGC 
CAGTTCCCCG AGTACCCACC AGATGGGGCC GCCAAGCTGG AGGTCCCGGC AGAGAGATCT 
CCTCGCAGAC GGAGCATCTC TGGGACCAGC ACATCTGAGA AAACCAACTC CATGGATGCT 
GCAAATACCT CTCCTTTCAA AGTATCGGGC TTCTTCAGTA AACGTCTGAA AGGGTCCATC 
AAGAGGACAA AGAGTCAGTC AAAGCTGGAC AGGAACACAA GTTTCCGCCT CCCATCCCTC 
CGCAATGCTG ATGACAGGTC ACGTGGGCTG CCGAAGCTGA AGGAGTCCCG CTCTCATGAG 
TCCCTGCTGA GCCCCGGCAG CGCAGTGGAA GCCCTGGACC TTGGGGCAGA AGAAAAGGTC 
TTTGTAAAGC CACTGCACAG CAGCATCCTC GGACAGGACT TCTGCTTTGA GGTAACCTAC 
TCCAGCGGCA GTAAATGCTT CAGCTGTTCC TCTGCTGCAG AGAGGGATAA GTGGATGGAA 
AACCTACGCA GAACAGTGCA GCCGAATAAG GACAACTGCC GTCGAGCTGA AAATGTGCTC 
CGTCTCTGGA TCATTGAGGC AAAGGACCTG GCCCCCAAGA AGAAATACTT CTGTGAGCTG 
TGCCTTGATG ACACTCTCTT TGCCCGCACC ACCAGCAAAA CGAAAGCAGA TAACATTTTC 
TGGGGGGAGC ACTTTGAGTT CTACGGCCTC CCAGCTCTTC ACAGCATCAC GGTTCACATC 
TACAAGGATG TGGAGAAGAA AAAGAAAAAG GACAAAAACA ATTACGTCGG CCTGGTCAAC 
ATTCCCATGG CCACTGTAAC GGGGCGCCAG TTTGTGGAAA AATGGTACCC AGTCAGCACA 
CCTACACCCA ACAAAGGGAA ATCAGGAGGC CCTTCCATTC GCATCAAGTC GCGTTATCAG 
ACCATCACCA TCTTACCCAT GGAGCAATAC AAGGAGTTTG CAGAGTTTGT TACCAGCAAC 
TACACGATGC TGTGCTCTGT GCTGGAGCCA GTCATCAGCG TAAGGAATAA GGAAGAGATG 
GCTTGTGCAA TGGTGCACAT TCTTCAGAGC ACGGGGAGAG CCAAGGACTT CCTAACGGAT 
CTGGTGATGT CTGAGGTGGA TCGCTGCGGG GAACACGATG TCTTGATCTT CAGGGAGAAC 
ACACTTGCTA CCAAAGCTAT TGAGGAATAC CTGAAGTTGG TAGGGCAGAA GTATCTCCAT 
GATGCGCTAG GGGAGTTTAT CAAGGCTTTG TATGAGTCAG ATGAAAACTG TGAAGTGGAC 
CCCAGCAAAT GTTCATCCAG TGAATTAACA GACCATCAGA GCAACCTGAA AATGTGTTGT 
GAGCTGGCCT TCTGCAAGAT TATCAATTCC TACTGCGTGT TCCCTCGTGA GCTGAAGGAG 
GTGTTTGCAT CTTGGAAGCA GCAGTGCCTG AGCAGGGGCA AGCAGGACAT CAGCGAGCGG 
CTGATCAGTG CCTCCCTGTT CCTCCGCTTC CTCTGCCCCG CCATCATGTC CCCCAGCCTC 
TTCAGCCTCA TGCAGGAGTA TCCTGACGAC CGGACATCGC GCACGCTCAC ACTCATTGCT 
AAAGTCATTC AGAATCTCGC CAATTTTGCT AAGTTTGGTA ACAAGGAAGA GTACATGGCT 
TTCATGAACG ACTTTCTGGA ACATGAGTGG GGGGGAATGA AGCGATTTCT CCTGGAGATC 
TCTAATCCAG ATACCATCTC AAACATGCCT GGATTTGAGG GCTATATCGA CCTGGGCAGA 
GAGCTCTCAG TTTTGCATTC CCTCTTATGG GAGGTTGTGT CCCAACTTGA TAAGGCGACC 
GTGGCAAAAC TGGGACCTCT TCCTCGTATT CTTGCCGACA TCACCAAATC AATGACCAAC 
CCTACGCCAA TACAGCAGCA GCTGAGGCGT TTCACTGAGC ACAGCTCTAG TCCAAACGTC 
AGTGGCAGTC TCTCCTCAGG GCTTCAGAAG ATATTCGAGG ACCCCACTGA TGGTGACTTG 
CATAAACTGA AGTCTCCAAC CCAGGAAAAC ATAGATGGCT ATTTCAGGGG CAAGACCTTG 
CTGTTGGTTC AGCAGGCGTC CACCCAGAGC ATGACTTACT CAGATAAGGA CGAAAGAGAG 
AGCATCTTGC CCAACGGACG TAGCATCTCC CTCATGGACC TGCAGGATCC CCATACCGCT 
CACAGCGACC ACGCTTCCAT GATGCACGAC GTGCCTTTGC GCTTGGCCGG CAGCCAGCTC 
TCCATCACCC AGGTGGCCAA CATCAAGCAG CTGTGGGAGA CCCAGAGCAC GCCCCAAAGC 
GCTCCCCAGG TGAGGAGGCC GCTGCACCCG GCTTTGAACC AGCAGGGCAG CCTCCAGCCT 
CTGTCATTCC AGAACCCGGT GTACCACCTC AACAACCCAC CTGCGCCGTT GCCCAAGGCC 
TCCGTGGACT CCAGCCTGGA GAACCTGAGC ACCGCCAGCT CCCAGAGCCG AAGCAACAGC 
GAAGACTTCA AGCTCAGCGG GCCCAGCAAC AGCAGCATGG AGGACTTCAC CAAGCGCAGC 
ACGCAGAGCG AGGACTTCTC TAGGCGGCAC ACGGTCCCGG ACAAGCACGT CCCCATCGCG 
TTGCCCCGCC AGAACAGCAG CAGCCAGGCA CAGATCCGCA AAATGGACCA GGCCGGGTTA 
GGGGCCAGGG CCAAAGCGCC GCAGTCGCTG CCGCACAGCG CGTCCCTGCG CAGCACGGGC 
AGCATGTCGG CCGTGTCGGG GGCCGTGGTG ACTGAGCCCG TCCAGAACGG CAGCCGCTCC 
CGGCAGCAGT CGTCCTCCTC CAGGGAGAGC CCTGTGCCGA AGGTGAGGGC CATTCAGAGG 
CAGCAAACGC AGCAGGTCCA GTCTCCTGTG GACTCAGCCA CAATGTCACC AGTGGAAAGG 
ACAGCAGCGT GGGTTCTCAA TAACGGGCAG TACGAGGAGG TCGAGGAGGA CACAGAGCAG 
AACCCAGATG AAGCCAAGCA TGCTGAGAAG TACGAACAAG AGATAGCAAA GCTGAAGGAA 
CGCTTGAAAG TGTCGAGCCG CCGGCTGGAG GAGTACGAGC GGCGGCTCCT GGTGCAGGAG 
CAGCAAATGC AGAAGCTGCT GATGGAGTAC AAGTCCAGGC TGGAGGACAG CGAGGAGAGA 
CTGCGCAGGC AACAGGAGGA GAAGGACAGC CAGATGAAAA GCATTATCAG CAGGCTCATG 
GCAGTTGAAG AGGAACTGAA GAAAGATCAT GCAGAGATGC AAGCTGTCAT CGATGCAAAG 
CAGAAAATTA TTGATGCACA GGAGAAGCGG ATCGTGTCCC TGGACTCTGC CAACACGCGG 
CTGATGAGTG CCCTGACGCA GGTGAAGGAG CGCTACAGCA TGCAAGTCCG CAATGGCATC 
TCCCCCACCA ACCCCACCAA GCTTTCCATC ACGGAGAATG GTGAATTCAA AAACAGCAGC 
TGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_015290512.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015145998.1 Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X5, mRNA.

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CloneID	OGa39043
Clone ID Related Accession (Same CDS sequence)	XM_015290512.1
Accession Version	XM_015290512.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4266bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	ras GTPase-activating protein nGAP isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGCTT TTAAAAAGTA TTACAGTCTC TCTCCCAGGA CCTTTAGGAG GTCTTTGTCG 
GAGCAAGATG CTGGCACCGC TGCCTTAAAG GACTATAATT GGTACCACCA AATGAGAAGC 
AAGAAGGGGA GCAAACAAAC AGCTTTGGAC CGAGAGAAAG CACTCGGAGC GTACAGGTGG 
CAAACACATC CCCGTCTTCC CTGGAGGACA AGCAGAAGAG CACACTCTTG CAGCCTCAGA 
GGGGAAGGTA ATGATTCGAG TCTTGTCAAA AGTTCTGCAA GCATGGATGC TACCAGCGCC 
GACGATCTCC ACCAAGACTC AACGGAGATG CTCTTCCAGG CAAGGACCAG ATCACCGCGG 
GTTCCCTTCA ACCGCTCCCT GTCTGAACCT GTGCCGCACT CTGCAAACAG GAATGCCAAG 
CCCTTTCTGC AGGCGGTGCG CTCTGTGGAT TGTGAGCAGC ACAGCTATTT TGTCCGTGGC 
ATCTTCTCAA CTCTCTCAAG TGGCTTTTCA AAGATAATAA ATCGAAGGGG TGAGCAGCCC 
CCCACTGAGC CACTGGAGGA GTGGAAACCA GCCGGTAGTC AAATTGACCC GTGCCCAGAT 
GTAAAAGGAC CACCTACGCA CAGGCTGTCC TGCGGCCAGT CCCCCTACAC CGAGACGACA 
ACATGGGAGA GGAAGTACTG CATCCTGACT GACAGCCAGC TGGTGCTGCT CAACAGGGAG 
AAGGAGGTGC CCACCGAGGG GTTGCAGGAG CCGCAGGATG CAACCAAAGG CCGCTGTCTG 
CGCCGCACGG TGAGCGTGCC CTCCGAGGGC CAGTTCCCCG AGTACCCACC AGATGGGGCC 
GCCAAGCTGG AGGTCCCGGC AGAGAGATCT CCTCGCAGAC GGAGCATCTC TGGGACCAGC 
ACATCTGAGA AAACCAACTC CATGGATGCT GCAAATACCT CTCCTTTCAA AGTATCGGGC 
TTCTTCAGTA AACGTCTGAA AGGGTCCATC AAGAGGACAA AGAGTCAGTC AAAGCTGGAC 
AGGAACACAA GTTTCCGCCT CCCATCCCTC CGCAATGCTG ATGACAGGTC ACGTGGGCTG 
CCGAAGCTGA AGGAGTCCCG CTCTCATGAG TCCCTGCTGA GCCCCGGCAG CGCAGTGGAA 
GCCCTGGACC TTGGGGCAGA AGAAAAGGTC TTTGTAAAGC CACTGCACAG CAGCATCCTC 
GGACAGGACT TCTGCTTTGA GGTAACCTAC TCCAGCGGCA GTAAATGCTT CAGCTGTTCC 
TCTGCTGCAG AGAGGGATAA GTGGATGGAA AACCTACGCA GAACAGTGCA GCCGAATAAG 
GACAACTGCC GTCGAGCTGA AAATGTGCTC CGTCTCTGGA TCATTGAGGC AAAGGACCTG 
GCCCCCAAGA AGAAATACTT CTGTGAGCTG TGCCTTGATG ACACTCTCTT TGCCCGCACC 
ACCAGCAAAA CGAAAGCAGA TAACATTTTC TGGGGGGAGC ACTTTGAGTT CTACGGCCTC 
CCAGCTCTTC ACAGCATCAC GGTTCACATC TACAAGGATG TGGAGAAGAA AAAGAAAAAG 
GACAAAAACA ATTACGTCGG CCTGGTCAAC ATTCCCATGG CCACTGTAAC GGGGCGCCAG 
TTTGTGGAAA AATGGTACCC AGTCAGCACA CCTACACCCA ACAAAGGGAA ATCAGGAGGC 
CCTTCCATTC GCATCAAGTC GCGTTATCAG ACCATCACCA TCTTACCCAT GGAGCAATAC 
AAGGAGTTTG CAGAGTTTGT TACCAGCAAC TACACGATGC TGTGCTCTGT GCTGGAGCCA 
GTCATCAGCG TAAGGAATAA GGAAGAGATG GCTTGTGCAA TGGTGCACAT TCTTCAGAGC 
ACGGGGAGAG CCAAGGACTT CCTAACGGAT CTGGTGATGT CTGAGGTGGA TCGCTGCGGG 
GAACACGATG TCTTGATCTT CAGGGAGAAC ACACTTGCTA CCAAAGCTAT TGAGGAATAC 
CTGAAGTTGG TAGGGCAGAA GTATCTCCAT GATGCGCTAG GGGAGTTTAT CAAGGCTTTG 
TATGAGTCAG ATGAAAACTG TGAAGTGGAC CCCAGCAAAT GTTCATCCAG TGAATTAACA 
GACCATCAGA GCAACCTGAA AATGTGTTGT GAGCTGGCCT TCTGCAAGAT TATCAATTCC 
TACTGCGTGT TCCCTCGTGA GCTGAAGGAG GTGTTTGCAT CTTGGAAGCA GCAGTGCCTG 
AGCAGGGGCA AGCAGGACAT CAGCGAGCGG CTGATCAGTG CCTCCCTGTT CCTCCGCTTC 
CTCTGCCCCG CCATCATGTC CCCCAGCCTC TTCAGCCTCA TGCAGGAGTA TCCTGACGAC 
CGGACATCGC GCACGCTCAC ACTCATTGCT AAAGTCATTC AGAATCTCGC CAATTTTGCT 
AAGTTTGGTA ACAAGGAAGA GTACATGGCT TTCATGAACG ACTTTCTGGA ACATGAGTGG 
GGGGGAATGA AGCGATTTCT CCTGGAGATC TCTAATCCAG ATACCATCTC AAACATGCCT 
GGATTTGAGG GCTATATCGA CCTGGGCAGA GAGCTCTCAG TTTTGCATTC CCTCTTATGG 
GAGGTTGTGT CCCAACTTGA TAAGGGTGAA AATTCCTTCC TACAGGCGAC CGTGGCAAAA 
CTGGGACCTC TTCCTCGTAT TCTTGCCGAC ATCACCAAAT CAATGACCAA CCCTACGCCA 
ATACAGCAGC AGCTGAGGCG TTTCACTGAG CACAGCTCTA GTCCAAACGT CAGTGGCAGT 
CTCTCCTCAG GGCTTCAGAA GATATTCGAG GACCCCACTG ATGGTGACTT GCATAAACTG 
AAGTCTCCAA CCCAGGAAAA CATAGATGGC TATTTCAGGG GCAAGACCTT GCTGTTGGTT 
CAGCAGGCGT CCACCCAGAG CATGACTTAC TCAGATAAGG ACGAAAGAGA GAGCATCTTG 
CCCAACGGAC GTAGCATCTC CCTCATGGAC CTGCAGGATC CCCATACCGC TCACAGCGAC 
CACGCTTCCA TGATGCACGA CGTGCCTTTG CGCTTGGCCG GCAGCCAGCT CTCCATCACC 
CAGGTGGCCA ACATCAAGCA GCTGTGGGAG ACCCAGAGCA CGCCCCAAAG CGCTCCCCAG 
GTGAGGAGGC CGCTGCACCC GGCTTTGAAC CAGCAGGGCA GCCTCCAGCC TCTGTCATTC 
CAGAACCCGG TGTACCACCT CAACAACCCA CCTGCGCCGT TGCCCAAGGC CTCCGTGGAC 
TCCAGCCTGG AGAACCTGAG CACCGCCAGC TCCCAGAGCC GAAGCAACAG CGAAGACTTC 
AAGCTCAGCG GGCCCAGCAA CAGCAGCATG GAGGACTTCA CCAAGCGCAG CACGCAGAGC 
GAGGACTTCT CTAGGCGGCA CACGGTCCCG GACAAGCACG TCCCCATCGC GTTGCCCCGC 
CAGAACAGCA GCAGCCAGGC ACAGATCCGC AAAATGGACC AGGCCGGGTT AGGGGCCAGG 
GCCAAAGCGC CGCAGTCGCT GCCGCACAGC GCGTCCCTGC GCAGCACGGG CAGCATGTCG 
GCCGTGTCGG GGGCCGTGGT GACTGAGCCC GTCCAGAACG GCAGCCGCTC CCGGCAGCAG 
TCGTCCTCCT CCAGGGAGAG CCCTGTGCCG AAGGTGAGGG CCATTCAGAG GCAGCAAACG 
CAGCAGGTCC AGTCTCCTGT GGACTCAGCC ACAATGTCAC CAGTGGAAAG GACAGCAGCG 
TGGGTTCTCA ATAACGGGCA GTACGAGGAG GTCGAGGAGG ACACAGAGCA GAACCCAGAT 
GAAGCCAAGC ATGCTGAGAA GTACGAACAA GAGATAGCAA AGCTGAAGGA ACGCTTGAAA 
GTGTCGAGCC GCCGGCTGGA GGAGTACGAG CGGCGGCTCC TGGTGCAGGA GCAGCAAATG 
CAGAAGCTGC TGATGGAGTA CAAGTCCAGG CTGGAGGACA GCGAGGAGAG ACTGCGCAGG 
CAACAGGAGG AGAAGGACAG CCAGATGAAA AGCATTATCA GCAGGCTCAT GGCAGTTGAA 
GAGGAACTGA AGAAAGATCA TGCAGAGATG CAAGCTGTCA TCGATGCAAA GCAGAAAATT 
ATTGATGCAC AGGTCATTAA TGGGAATGAG ATAATTCAAA CAGGAGAAGC GGATCGTGTC 
CCTGGACTCT GCCAACACGC GGCTGATGAG TGCCCTGACG CAGGTGAAGG AGCGCTACAG 
CATGCAAGTC CGCAATGGCA TCTCCCCCAC CAACCCCACC AAGCTTTCCA TCACGGAGAA 
TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015145998.1
CDS	637..4902
Translation	MSAFKKYYSLSPRTFRRSLSEQDAGTAALKDYNWYHQMRSKKGSKQTALDREKALGAYRWQTHPRLPWRTSRRAHSCSLRGEGNDSSLVKSSASMDATSADDLHQDSTEMLFQARTRSPRVPFNRSLSEPVPHSANRNAKPFLQAVRSVDCEQHSYFVRGIFSTLSSGFSKIINRRGEQPPTEPLEEWKPAGSQIDPCPDVKGPPTHRLSCGQSPYTETTTWERKYCILTDSQLVLLNREKEVPTEGLQEPQDATKGRCLRRTVSVPSEGQFPEYPPDGAAKLEVPAERSPRRRSISGTSTSEKTNSMDAANTSPFKVSGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNADDRSRGLPKLKESRSHESLLSPGSAVEALDLGAEEKVFVKPLHSSILGQDFCFEVTYSSGSKCFSCSSAAERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYGLPALHSITVHIYKDVEKKKKKDKNNYVGLVNIPMATVTGRQFVEKWYPVSTPTPNKGKSGGPSIRIKSRYQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEEMACAMVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTLATKAIEEYLKLVGQKYLHDALGEFIKALYESDENCEVDPSKCSSSELTDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCLSRGKQDISERLISASLFLRFLCPAIMSPSLFSLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNMPGFEGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVAKLGPLPRILADITKSMTNPTPIQQQLRRFTEHSSSPNVSGSLSSGLQKIFEDPTDGDLHKLKSPTQENIDGYFRGKTLLLVQQASTQSMTYSDKDERESILPNGRSISLMDLQDPHTAHSDHASMMHDVPLRLAGSQLSITQVANIKQLWETQSTPQSAPQVRRPLHPALNQQGSLQPLSFQNPVYHLNNPPAPLPKASVDSSLENLSTASSQSRSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTVPDKHVPIALPRQNSSSQAQIRKMDQAGLGARAKAPQSLPHSASLRSTGSMSAVSGAVVTEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEVEEDTEQNPDEAKHAEKYEQEIAKLKERLKVSSRRLEEYERRLLVQEQQMQKLLMEYKSRLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQVINGNEIIQTGEADRVPGLCQHAADECPDAGEGALQHASPQWHLPHQPHQAFHHGEW

Target ORF information:

RefSeq Version	XM_015290512.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015290512.1

ORF Insert Sequence:

ATGAGTGCTT TTAAAAAGTA TTACAGTCTC TCTCCCAGGA CCTTTAGGAG GTCTTTGTCG 
GAGCAAGATG CTGGCACCGC TGCCTTAAAG GACTATAATT GGTACCACCA AATGAGAAGC 
AAGAAGGGGA GCAAACAAAC AGCTTTGGAC CGAGAGAAAG CACTCGGAGC GTACAGGTGG 
CAAACACATC CCCGTCTTCC CTGGAGGACA AGCAGAAGAG CACACTCTTG CAGCCTCAGA 
GGGGAAGGTA ATGATTCGAG TCTTGTCAAA AGTTCTGCAA GCATGGATGC TACCAGCGCC 
GACGATCTCC ACCAAGACTC AACGGAGATG CTCTTCCAGG CAAGGACCAG ATCACCGCGG 
GTTCCCTTCA ACCGCTCCCT GTCTGAACCT GTGCCGCACT CTGCAAACAG GAATGCCAAG 
CCCTTTCTGC AGGCGGTGCG CTCTGTGGAT TGTGAGCAGC ACAGCTATTT TGTCCGTGGC 
ATCTTCTCAA CTCTCTCAAG TGGCTTTTCA AAGATAATAA ATCGAAGGGG TGAGCAGCCC 
CCCACTGAGC CACTGGAGGA GTGGAAACCA GCCGGTAGTC AAATTGACCC GTGCCCAGAT 
GTAAAAGGAC CACCTACGCA CAGGCTGTCC TGCGGCCAGT CCCCCTACAC CGAGACGACA 
ACATGGGAGA GGAAGTACTG CATCCTGACT GACAGCCAGC TGGTGCTGCT CAACAGGGAG 
AAGGAGGTGC CCACCGAGGG GTTGCAGGAG CCGCAGGATG CAACCAAAGG CCGCTGTCTG 
CGCCGCACGG TGAGCGTGCC CTCCGAGGGC CAGTTCCCCG AGTACCCACC AGATGGGGCC 
GCCAAGCTGG AGGTCCCGGC AGAGAGATCT CCTCGCAGAC GGAGCATCTC TGGGACCAGC 
ACATCTGAGA AAACCAACTC CATGGATGCT GCAAATACCT CTCCTTTCAA AGTATCGGGC 
TTCTTCAGTA AACGTCTGAA AGGGTCCATC AAGAGGACAA AGAGTCAGTC AAAGCTGGAC 
AGGAACACAA GTTTCCGCCT CCCATCCCTC CGCAATGCTG ATGACAGGTC ACGTGGGCTG 
CCGAAGCTGA AGGAGTCCCG CTCTCATGAG TCCCTGCTGA GCCCCGGCAG CGCAGTGGAA 
GCCCTGGACC TTGGGGCAGA AGAAAAGGTC TTTGTAAAGC CACTGCACAG CAGCATCCTC 
GGACAGGACT TCTGCTTTGA GGTAACCTAC TCCAGCGGCA GTAAATGCTT CAGCTGTTCC 
TCTGCTGCAG AGAGGGATAA GTGGATGGAA AACCTACGCA GAACAGTGCA GCCGAATAAG 
GACAACTGCC GTCGAGCTGA AAATGTGCTC CGTCTCTGGA TCATTGAGGC AAAGGACCTG 
GCCCCCAAGA AGAAATACTT CTGTGAGCTG TGCCTTGATG ACACTCTCTT TGCCCGCACC 
ACCAGCAAAA CGAAAGCAGA TAACATTTTC TGGGGGGAGC ACTTTGAGTT CTACGGCCTC 
CCAGCTCTTC ACAGCATCAC GGTTCACATC TACAAGGATG TGGAGAAGAA AAAGAAAAAG 
GACAAAAACA ATTACGTCGG CCTGGTCAAC ATTCCCATGG CCACTGTAAC GGGGCGCCAG 
TTTGTGGAAA AATGGTACCC AGTCAGCACA CCTACACCCA ACAAAGGGAA ATCAGGAGGC 
CCTTCCATTC GCATCAAGTC GCGTTATCAG ACCATCACCA TCTTACCCAT GGAGCAATAC 
AAGGAGTTTG CAGAGTTTGT TACCAGCAAC TACACGATGC TGTGCTCTGT GCTGGAGCCA 
GTCATCAGCG TAAGGAATAA GGAAGAGATG GCTTGTGCAA TGGTGCACAT TCTTCAGAGC 
ACGGGGAGAG CCAAGGACTT CCTAACGGAT CTGGTGATGT CTGAGGTGGA TCGCTGCGGG 
GAACACGATG TCTTGATCTT CAGGGAGAAC ACACTTGCTA CCAAAGCTAT TGAGGAATAC 
CTGAAGTTGG TAGGGCAGAA GTATCTCCAT GATGCGCTAG GGGAGTTTAT CAAGGCTTTG 
TATGAGTCAG ATGAAAACTG TGAAGTGGAC CCCAGCAAAT GTTCATCCAG TGAATTAACA 
GACCATCAGA GCAACCTGAA AATGTGTTGT GAGCTGGCCT TCTGCAAGAT TATCAATTCC 
TACTGCGTGT TCCCTCGTGA GCTGAAGGAG GTGTTTGCAT CTTGGAAGCA GCAGTGCCTG 
AGCAGGGGCA AGCAGGACAT CAGCGAGCGG CTGATCAGTG CCTCCCTGTT CCTCCGCTTC 
CTCTGCCCCG CCATCATGTC CCCCAGCCTC TTCAGCCTCA TGCAGGAGTA TCCTGACGAC 
CGGACATCGC GCACGCTCAC ACTCATTGCT AAAGTCATTC AGAATCTCGC CAATTTTGCT 
AAGTTTGGTA ACAAGGAAGA GTACATGGCT TTCATGAACG ACTTTCTGGA ACATGAGTGG 
GGGGGAATGA AGCGATTTCT CCTGGAGATC TCTAATCCAG ATACCATCTC AAACATGCCT 
GGATTTGAGG GCTATATCGA CCTGGGCAGA GAGCTCTCAG TTTTGCATTC CCTCTTATGG 
GAGGTTGTGT CCCAACTTGA TAAGGGTGAA AATTCCTTCC TACAGGCGAC CGTGGCAAAA 
CTGGGACCTC TTCCTCGTAT TCTTGCCGAC ATCACCAAAT CAATGACCAA CCCTACGCCA 
ATACAGCAGC AGCTGAGGCG TTTCACTGAG CACAGCTCTA GTCCAAACGT CAGTGGCAGT 
CTCTCCTCAG GGCTTCAGAA GATATTCGAG GACCCCACTG ATGGTGACTT GCATAAACTG 
AAGTCTCCAA CCCAGGAAAA CATAGATGGC TATTTCAGGG GCAAGACCTT GCTGTTGGTT 
CAGCAGGCGT CCACCCAGAG CATGACTTAC TCAGATAAGG ACGAAAGAGA GAGCATCTTG 
CCCAACGGAC GTAGCATCTC CCTCATGGAC CTGCAGGATC CCCATACCGC TCACAGCGAC 
CACGCTTCCA TGATGCACGA CGTGCCTTTG CGCTTGGCCG GCAGCCAGCT CTCCATCACC 
CAGGTGGCCA ACATCAAGCA GCTGTGGGAG ACCCAGAGCA CGCCCCAAAG CGCTCCCCAG 
GTGAGGAGGC CGCTGCACCC GGCTTTGAAC CAGCAGGGCA GCCTCCAGCC TCTGTCATTC 
CAGAACCCGG TGTACCACCT CAACAACCCA CCTGCGCCGT TGCCCAAGGC CTCCGTGGAC 
TCCAGCCTGG AGAACCTGAG CACCGCCAGC TCCCAGAGCC GAAGCAACAG CGAAGACTTC 
AAGCTCAGCG GGCCCAGCAA CAGCAGCATG GAGGACTTCA CCAAGCGCAG CACGCAGAGC 
GAGGACTTCT CTAGGCGGCA CACGGTCCCG GACAAGCACG TCCCCATCGC GTTGCCCCGC 
CAGAACAGCA GCAGCCAGGC ACAGATCCGC AAAATGGACC AGGCCGGGTT AGGGGCCAGG 
GCCAAAGCGC CGCAGTCGCT GCCGCACAGC GCGTCCCTGC GCAGCACGGG CAGCATGTCG 
GCCGTGTCGG GGGCCGTGGT GACTGAGCCC GTCCAGAACG GCAGCCGCTC CCGGCAGCAG 
TCGTCCTCCT CCAGGGAGAG CCCTGTGCCG AAGGTGAGGG CCATTCAGAG GCAGCAAACG 
CAGCAGGTCC AGTCTCCTGT GGACTCAGCC ACAATGTCAC CAGTGGAAAG GACAGCAGCG 
TGGGTTCTCA ATAACGGGCA GTACGAGGAG GTCGAGGAGG ACACAGAGCA GAACCCAGAT 
GAAGCCAAGC ATGCTGAGAA GTACGAACAA GAGATAGCAA AGCTGAAGGA ACGCTTGAAA 
GTGTCGAGCC GCCGGCTGGA GGAGTACGAG CGGCGGCTCC TGGTGCAGGA GCAGCAAATG 
CAGAAGCTGC TGATGGAGTA CAAGTCCAGG CTGGAGGACA GCGAGGAGAG ACTGCGCAGG 
CAACAGGAGG AGAAGGACAG CCAGATGAAA AGCATTATCA GCAGGCTCAT GGCAGTTGAA 
GAGGAACTGA AGAAAGATCA TGCAGAGATG CAAGCTGTCA TCGATGCAAA GCAGAAAATT 
ATTGATGCAC AGGTCATTAA TGGGAATGAG ATAATTCAAA CAGGAGAAGC GGATCGTGTC 
CCTGGACTCT GCCAACACGC GGCTGATGAG TGCCCTGACG CAGGTGAAGG AGCGCTACAG 
CATGCAAGTC CGCAATGGCA TCTCCCCCAC CAACCCCACC AAGCTTTCCA TCACGGAGAA 
TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_015290515.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015146001.1 Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OGa39047
Clone ID Related Accession (Same CDS sequence)	XM_015290515.1
Accession Version	XM_015290515.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	ras GTPase-activating protein nGAP isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAGCC TGGTGAGGAA GATGCTAGTG CAGACAAAGC CATGGAGCGA TGTAAAAGGA 
CCACCTACGC ACAGGCTGTC CTGCGGCCAG TCCCCCTACA CCGAGACGAC AACATGGGAG 
AGGAAGTACT GCATCCTGAC TGACAGCCAG CTGGTGCTGC TCAACAGGGA GAAGGAGGTG 
CCCACCGAGG GGTTGCAGGA GCCGCAGGAT GCAACCAAAG GCCGCTGTCT GCGCCGCACG 
GTGAGCGTGC CCTCCGAGGG CCAGTTCCCC GAGTACCCAC CAGATGGGGC CGCCAAGCTG 
GAGGTCCCGG CAGAGAGATC TCCTCGCAGA CGGAGCATCT CTGGGACCAG CACATCTGAG 
AAAACCAACT CCATGGATGC TGCAAATACC TCTCCTTTCA AAGTATCGGG CTTCTTCAGT 
AAACGTCTGA AAGGGTCCAT CAAGAGGACA AAGAGTCAGT CAAAGCTGGA CAGGAACACA 
AGTTTCCGCC TCCCATCCCT CCGCAATGCT GATGACAGGT CACGTGGGCT GCCGAAGCTG 
AAGGAGTCCC GCTCTCATGA GTCCCTGCTG AGCCCCGGCA GCGCAGTGGA AGCCCTGGAC 
CTTGGGGCAG AAGAAAAGGT CTTTGTAAAG CCACTGCACA GCAGCATCCT CGGACAGGAC 
TTCTGCTTTG AGGTAACCTA CTCCAGCGGC AGTAAATGCT TCAGCTGTTC CTCTGCTGCA 
GAGAGGGATA AGTGGATGGA AAACCTACGC AGAACAGTGC AGCCGAATAA GGACAACTGC 
CGTCGAGCTG AAAATGTGCT CCGTCTCTGG ATCATTGAGG CAAAGGACCT GGCCCCCAAG 
AAGAAATACT TCTGTGAGCT GTGCCTTGAT GACACTCTCT TTGCCCGCAC CACCAGCAAA 
ACGAAAGCAG ATAACATTTT CTGGGGGGAG CACTTTGAGT TCTACGGCCT CCCAGCTCTT 
CACAGCATCA CGGTTCACAT CTACAAGGAT GTGGAGAAGA AAAAGAAAAA GGACAAAAAC 
AATTACGTCG GCCTGGTCAA CATTCCCATG GCCACTGTAA CGGGGCGCCA GTTTGTGGAA 
AAATGGTACC CAGTCAGCAC ACCTACACCC AACAAAGGGA AATCAGGAGG CCCTTCCATT 
CGCATCAAGT CGCGTTATCA GACCATCACC ATCTTACCCA TGGAGCAATA CAAGGAGTTT 
GCAGAGTTTG TTACCAGCAA CTACACGATG CTGTGCTCTG TGCTGGAGCC AGTCATCAGC 
GTAAGGAATA AGGAAGAGAT GGCTTGTGCA ATGGTGCACA TTCTTCAGAG CACGGGGAGA 
GCCAAGGACT TCCTAACGGA TCTGGTGATG TCTGAGGTGG ATCGCTGCGG GGAACACGAT 
GTCTTGATCT TCAGGGAGAA CACACTTGCT ACCAAAGCTA TTGAGGAATA CCTGAAGTTG 
GTAGGGCAGA AGTATCTCCA TGATGCGCTA GGGGAGTTTA TCAAGGCTTT GTATGAGTCA 
GATGAAAACT GTGAAGTGGA CCCCAGCAAA TGTTCATCCA GTGAATTAAC AGACCATCAG 
AGCAACCTGA AAATGTGTTG TGAGCTGGCC TTCTGCAAGA TTATCAATTC CTACTGCGTG 
TTCCCTCGTG AGCTGAAGGA GGTGTTTGCA TCTTGGAAGC AGCAGTGCCT GAGCAGGGGC 
AAGCAGGACA TCAGCGAGCG GCTGATCAGT GCCTCCCTGT TCCTCCGCTT CCTCTGCCCC 
GCCATCATGT CCCCCAGCCT CTTCAGCCTC ATGCAGGAGT ATCCTGACGA CCGGACATCG 
CGCACGCTCA CACTCATTGC TAAAGTCATT CAGAATCTCG CCAATTTTGC TAAGTTTGGT 
AACAAGGAAG AGTACATGGC TTTCATGAAC GACTTTCTGG AACATGAGTG GGGGGGAATG 
AAGCGATTTC TCCTGGAGAT CTCTAATCCA GATACCATCT CAAACATGCC TGGATTTGAG 
GGCTATATCG ACCTGGGCAG AGAGCTCTCA GTTTTGCATT CCCTCTTATG GGAGGTTGTG 
TCCCAACTTG ATAAGGGTGA AAATTCCTTC CTACAGGCGA CCGTGGCAAA ACTGGGACCT 
CTTCCTCGTA TTCTTGCCGA CATCACCAAA TCAATGACCA ACCCTACGCC AATACAGCAG 
CAGCTGAGGC GTTTCACTGA GCACAGCTCT AGTCCAAACG TCAGTGGCAG TCTCTCCTCA 
GGGCTTCAGA AGATATTCGA GGACCCCACT GATGGTGACT TGCATAAACT GAAGTCTCCA 
ACCCAGGAAA ACATAGATGG CTATTTCAGG GGCAAGACCT TGCTGTTGGT TCAGCAGGCG 
TCCACCCAGA GCATGACTTA CTCAGATAAG GACGAAAGAG AGAGCATCTT GCCCAACGGA 
CGTAGCATCT CCCTCATGGA CCTGCAGGAT CCCCATACCG CTCACAGCGA CCACGCTTCC 
ATGATGCACG ACGTGCCTTT GCGCTTGGCC GGCAGCCAGC TCTCCATCAC CCAGGTGGCC 
AACATCAAGC AGCTGTGGGA GACCCAGAGC ACGCCCCAAA GCGCTCCCCA GGTGAGGAGG 
CCGCTGCACC CGGCTTTGAA CCAGCAGGGC AGCCTCCAGC CTCTGTCATT CCAGAACCCG 
GTGTACCACC TCAACAACCC ACCTGCGCCG TTGCCCAAGG CCTCCGTGGA CTCCAGCCTG 
GAGAACCTGA GCACCGCCAG CTCCCAGAGC CGAAGCAACA GCGAAGACTT CAAGCTCAGC 
GGGCCCAGCA ACAGCAGCAT GGAGGACTTC ACCAAGCGCA GCACGCAGAG CGAGGACTTC 
TCTAGGCGGC ACACGGTCCC GGACAAGCAC GTCCCCATCG CGTTGCCCCG CCAGAACAGC 
AGCAGCCAGG CACAGATCCG CAAAATGGAC CAGGCCGGGT TAGGGGCCAG GGCCAAAGCG 
CCGCAGTCGC TGCCGCACAG CGCGTCCCTG CGCAGCACGG GCAGCATGTC GGCCGTGTCG 
GGGGCCGTGG TGACTGAGCC CGTCCAGAAC GGCAGCCGCT CCCGGCAGCA GTCGTCCTCC 
TCCAGGGAGA GCCCTGTGCC GAAGGTGAGG GCCATTCAGA GGCAGCAAAC GCAGCAGGTC 
CAGTCTCCTG TGGACTCAGC CACAATGTCA CCAGTGGAAA GGACAGCAGC GTGGGTTCTC 
AATAACGGGC AGTACGAGGA GGTCGAGGAG GACACAGAGC AGAACCCAGA TGAAGCCAAG 
CATGCTGAGA AGTACGAACA AGAGATAGCA AAGCTGAAGG AACGCTTGAA AGTGTCGAGC 
CGCCGGCTGG AGGAGTACGA GCGGCGGCTC CTGGTGCAGG AGCAGCAAAT GCAGAAGCTG 
CTGATGGAGT ACAAGTCCAG GCTGGAGGAC AGCGAGGAGA GACTGCGCAG GCAACAGGAG 
GAGAAGGACA GCCAGATGAA AAGCATTATC AGCAGGCTCA TGGCAGTTGA AGAGGAACTG 
AAGAAAGATC ATGCAGAGAT GCAAGCTGTC ATCGATGCAA AGCAGAAAAT TATTGATGCA 
CAGGTCATTA ATGGGAATGA GATAATTCAA ACAGGAGAAG CGGATCGTGT CCCTGGACTC 
TGCCAACACG CGGCTGATGA GTGCCCTGAC GCAGGTGAAG GAGCGCTACA GCATGCAAGT 
CCGCAATGGC ATCTCCCCCA CCAACCCCAC CAAGCTTTCC ATCACGGAGA ATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015146001.1
CDS	151..3867
Translation	MASLVRKMLVQTKPWSDVKGPPTHRLSCGQSPYTETTTWERKYCILTDSQLVLLNREKEVPTEGLQEPQDATKGRCLRRTVSVPSEGQFPEYPPDGAAKLEVPAERSPRRRSISGTSTSEKTNSMDAANTSPFKVSGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNADDRSRGLPKLKESRSHESLLSPGSAVEALDLGAEEKVFVKPLHSSILGQDFCFEVTYSSGSKCFSCSSAAERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYGLPALHSITVHIYKDVEKKKKKDKNNYVGLVNIPMATVTGRQFVEKWYPVSTPTPNKGKSGGPSIRIKSRYQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEEMACAMVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTLATKAIEEYLKLVGQKYLHDALGEFIKALYESDENCEVDPSKCSSSELTDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCLSRGKQDISERLISASLFLRFLCPAIMSPSLFSLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNMPGFEGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVAKLGPLPRILADITKSMTNPTPIQQQLRRFTEHSSSPNVSGSLSSGLQKIFEDPTDGDLHKLKSPTQENIDGYFRGKTLLLVQQASTQSMTYSDKDERESILPNGRSISLMDLQDPHTAHSDHASMMHDVPLRLAGSQLSITQVANIKQLWETQSTPQSAPQVRRPLHPALNQQGSLQPLSFQNPVYHLNNPPAPLPKASVDSSLENLSTASSQSRSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTVPDKHVPIALPRQNSSSQAQIRKMDQAGLGARAKAPQSLPHSASLRSTGSMSAVSGAVVTEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEVEEDTEQNPDEAKHAEKYEQEIAKLKERLKVSSRRLEEYERRLLVQEQQMQKLLMEYKSRLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQVINGNEIIQTGEADRVPGLCQHAADECPDAGEGALQHASPQWHLPHQPHQAFHHGEW

Target ORF information:

RefSeq Version	XM_015290515.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015290515.1

ORF Insert Sequence:

ATGGCAAGCC TGGTGAGGAA GATGCTAGTG CAGACAAAGC CATGGAGCGA TGTAAAAGGA 
CCACCTACGC ACAGGCTGTC CTGCGGCCAG TCCCCCTACA CCGAGACGAC AACATGGGAG 
AGGAAGTACT GCATCCTGAC TGACAGCCAG CTGGTGCTGC TCAACAGGGA GAAGGAGGTG 
CCCACCGAGG GGTTGCAGGA GCCGCAGGAT GCAACCAAAG GCCGCTGTCT GCGCCGCACG 
GTGAGCGTGC CCTCCGAGGG CCAGTTCCCC GAGTACCCAC CAGATGGGGC CGCCAAGCTG 
GAGGTCCCGG CAGAGAGATC TCCTCGCAGA CGGAGCATCT CTGGGACCAG CACATCTGAG 
AAAACCAACT CCATGGATGC TGCAAATACC TCTCCTTTCA AAGTATCGGG CTTCTTCAGT 
AAACGTCTGA AAGGGTCCAT CAAGAGGACA AAGAGTCAGT CAAAGCTGGA CAGGAACACA 
AGTTTCCGCC TCCCATCCCT CCGCAATGCT GATGACAGGT CACGTGGGCT GCCGAAGCTG 
AAGGAGTCCC GCTCTCATGA GTCCCTGCTG AGCCCCGGCA GCGCAGTGGA AGCCCTGGAC 
CTTGGGGCAG AAGAAAAGGT CTTTGTAAAG CCACTGCACA GCAGCATCCT CGGACAGGAC 
TTCTGCTTTG AGGTAACCTA CTCCAGCGGC AGTAAATGCT TCAGCTGTTC CTCTGCTGCA 
GAGAGGGATA AGTGGATGGA AAACCTACGC AGAACAGTGC AGCCGAATAA GGACAACTGC 
CGTCGAGCTG AAAATGTGCT CCGTCTCTGG ATCATTGAGG CAAAGGACCT GGCCCCCAAG 
AAGAAATACT TCTGTGAGCT GTGCCTTGAT GACACTCTCT TTGCCCGCAC CACCAGCAAA 
ACGAAAGCAG ATAACATTTT CTGGGGGGAG CACTTTGAGT TCTACGGCCT CCCAGCTCTT 
CACAGCATCA CGGTTCACAT CTACAAGGAT GTGGAGAAGA AAAAGAAAAA GGACAAAAAC 
AATTACGTCG GCCTGGTCAA CATTCCCATG GCCACTGTAA CGGGGCGCCA GTTTGTGGAA 
AAATGGTACC CAGTCAGCAC ACCTACACCC AACAAAGGGA AATCAGGAGG CCCTTCCATT 
CGCATCAAGT CGCGTTATCA GACCATCACC ATCTTACCCA TGGAGCAATA CAAGGAGTTT 
GCAGAGTTTG TTACCAGCAA CTACACGATG CTGTGCTCTG TGCTGGAGCC AGTCATCAGC 
GTAAGGAATA AGGAAGAGAT GGCTTGTGCA ATGGTGCACA TTCTTCAGAG CACGGGGAGA 
GCCAAGGACT TCCTAACGGA TCTGGTGATG TCTGAGGTGG ATCGCTGCGG GGAACACGAT 
GTCTTGATCT TCAGGGAGAA CACACTTGCT ACCAAAGCTA TTGAGGAATA CCTGAAGTTG 
GTAGGGCAGA AGTATCTCCA TGATGCGCTA GGGGAGTTTA TCAAGGCTTT GTATGAGTCA 
GATGAAAACT GTGAAGTGGA CCCCAGCAAA TGTTCATCCA GTGAATTAAC AGACCATCAG 
AGCAACCTGA AAATGTGTTG TGAGCTGGCC TTCTGCAAGA TTATCAATTC CTACTGCGTG 
TTCCCTCGTG AGCTGAAGGA GGTGTTTGCA TCTTGGAAGC AGCAGTGCCT GAGCAGGGGC 
AAGCAGGACA TCAGCGAGCG GCTGATCAGT GCCTCCCTGT TCCTCCGCTT CCTCTGCCCC 
GCCATCATGT CCCCCAGCCT CTTCAGCCTC ATGCAGGAGT ATCCTGACGA CCGGACATCG 
CGCACGCTCA CACTCATTGC TAAAGTCATT CAGAATCTCG CCAATTTTGC TAAGTTTGGT 
AACAAGGAAG AGTACATGGC TTTCATGAAC GACTTTCTGG AACATGAGTG GGGGGGAATG 
AAGCGATTTC TCCTGGAGAT CTCTAATCCA GATACCATCT CAAACATGCC TGGATTTGAG 
GGCTATATCG ACCTGGGCAG AGAGCTCTCA GTTTTGCATT CCCTCTTATG GGAGGTTGTG 
TCCCAACTTG ATAAGGGTGA AAATTCCTTC CTACAGGCGA CCGTGGCAAA ACTGGGACCT 
CTTCCTCGTA TTCTTGCCGA CATCACCAAA TCAATGACCA ACCCTACGCC AATACAGCAG 
CAGCTGAGGC GTTTCACTGA GCACAGCTCT AGTCCAAACG TCAGTGGCAG TCTCTCCTCA 
GGGCTTCAGA AGATATTCGA GGACCCCACT GATGGTGACT TGCATAAACT GAAGTCTCCA 
ACCCAGGAAA ACATAGATGG CTATTTCAGG GGCAAGACCT TGCTGTTGGT TCAGCAGGCG 
TCCACCCAGA GCATGACTTA CTCAGATAAG GACGAAAGAG AGAGCATCTT GCCCAACGGA 
CGTAGCATCT CCCTCATGGA CCTGCAGGAT CCCCATACCG CTCACAGCGA CCACGCTTCC 
ATGATGCACG ACGTGCCTTT GCGCTTGGCC GGCAGCCAGC TCTCCATCAC CCAGGTGGCC 
AACATCAAGC AGCTGTGGGA GACCCAGAGC ACGCCCCAAA GCGCTCCCCA GGTGAGGAGG 
CCGCTGCACC CGGCTTTGAA CCAGCAGGGC AGCCTCCAGC CTCTGTCATT CCAGAACCCG 
GTGTACCACC TCAACAACCC ACCTGCGCCG TTGCCCAAGG CCTCCGTGGA CTCCAGCCTG 
GAGAACCTGA GCACCGCCAG CTCCCAGAGC CGAAGCAACA GCGAAGACTT CAAGCTCAGC 
GGGCCCAGCA ACAGCAGCAT GGAGGACTTC ACCAAGCGCA GCACGCAGAG CGAGGACTTC 
TCTAGGCGGC ACACGGTCCC GGACAAGCAC GTCCCCATCG CGTTGCCCCG CCAGAACAGC 
AGCAGCCAGG CACAGATCCG CAAAATGGAC CAGGCCGGGT TAGGGGCCAG GGCCAAAGCG 
CCGCAGTCGC TGCCGCACAG CGCGTCCCTG CGCAGCACGG GCAGCATGTC GGCCGTGTCG 
GGGGCCGTGG TGACTGAGCC CGTCCAGAAC GGCAGCCGCT CCCGGCAGCA GTCGTCCTCC 
TCCAGGGAGA GCCCTGTGCC GAAGGTGAGG GCCATTCAGA GGCAGCAAAC GCAGCAGGTC 
CAGTCTCCTG TGGACTCAGC CACAATGTCA CCAGTGGAAA GGACAGCAGC GTGGGTTCTC 
AATAACGGGC AGTACGAGGA GGTCGAGGAG GACACAGAGC AGAACCCAGA TGAAGCCAAG 
CATGCTGAGA AGTACGAACA AGAGATAGCA AAGCTGAAGG AACGCTTGAA AGTGTCGAGC 
CGCCGGCTGG AGGAGTACGA GCGGCGGCTC CTGGTGCAGG AGCAGCAAAT GCAGAAGCTG 
CTGATGGAGT ACAAGTCCAG GCTGGAGGAC AGCGAGGAGA GACTGCGCAG GCAACAGGAG 
GAGAAGGACA GCCAGATGAA AAGCATTATC AGCAGGCTCA TGGCAGTTGA AGAGGAACTG 
AAGAAAGATC ATGCAGAGAT GCAAGCTGTC ATCGATGCAA AGCAGAAAAT TATTGATGCA 
CAGGTCATTA ATGGGAATGA GATAATTCAA ACAGGAGAAG CGGATCGTGT CCCTGGACTC 
TGCCAACACG CGGCTGATGA GTGCCCTGAC GCAGGTGAAG GAGCGCTACA GCATGCAAGT 
CCGCAATGGC ATCTCCCCCA CCAACCCCAC CAAGCTTTCC ATCACGGAGA ATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_015290516.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015146002.1 Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OGa39048
Clone ID Related Accession (Same CDS sequence)	XM_015290516.1
Accession Version	XM_015290516.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	ras GTPase-activating protein nGAP isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCTCC TTTCTCCCGG CGATGTGGCC GAGTGGCTGG ATAGCAGCAC AGAAGATGAG 
AAAAGCCTTC TGTCGTTTCT CGAGGTCCCG GCAGAGAGAT CTCCTCGCAG ACGGAGCATC 
TCTGGGACCA GCACATCTGA GAAAACCAAC TCCATGGATG CTGCAAATAC CTCTCCTTTC 
AAAGTATCGG GCTTCTTCAG TAAACGTCTG AAAGGGTCCA TCAAGAGGAC AAAGAGTCAG 
TCAAAGCTGG ACAGGAACAC AAGTTTCCGC CTCCCATCCC TCCGCAATGC TGATGACAGG 
TCACGTGGGC TGCCGAAGCT GAAGGAGTCC CGCTCTCATG AGTCCCTGCT GAGCCCCGGC 
AGCGCAGTGG AAGCCCTGGA CCTTGGGGCA GAAGAAAAGG TCTTTGTAAA GCCACTGCAC 
AGCAGCATCC TCGGACAGGA CTTCTGCTTT GAGGTAACCT ACTCCAGCGG CAGTAAATGC 
TTCAGCTGTT CCTCTGCTGC AGAGAGGGAT AAGTGGATGG AAAACCTACG CAGAACAGTG 
CAGCCGAATA AGGACAACTG CCGTCGAGCT GAAAATGTGC TCCGTCTCTG GATCATTGAG 
GCAAAGGACC TGGCCCCCAA GAAGAAATAC TTCTGTGAGC TGTGCCTTGA TGACACTCTC 
TTTGCCCGCA CCACCAGCAA AACGAAAGCA GATAACATTT TCTGGGGGGA GCACTTTGAG 
TTCTACGGCC TCCCAGCTCT TCACAGCATC ACGGTTCACA TCTACAAGGA TGTGGAGAAG 
AAAAAGAAAA AGGACAAAAA CAATTACGTC GGCCTGGTCA ACATTCCCAT GGCCACTGTA 
ACGGGGCGCC AGTTTGTGGA AAAATGGTAC CCAGTCAGCA CACCTACACC CAACAAAGGG 
AAATCAGGAG GCCCTTCCAT TCGCATCAAG TCGCGTTATC AGACCATCAC CATCTTACCC 
ATGGAGCAAT ACAAGGAGTT TGCAGAGTTT GTTACCAGCA ACTACACGAT GCTGTGCTCT 
GTGCTGGAGC CAGTCATCAG CGTAAGGAAT AAGGAAGAGA TGGCTTGTGC AATGGTGCAC 
ATTCTTCAGA GCACGGGGAG AGCCAAGGAC TTCCTAACGG ATCTGGTGAT GTCTGAGGTG 
GATCGCTGCG GGGAACACGA TGTCTTGATC TTCAGGGAGA ACACACTTGC TACCAAAGCT 
ATTGAGGAAT ACCTGAAGTT GGTAGGGCAG AAGTATCTCC ATGATGCGCT AGGGGAGTTT 
ATCAAGGCTT TGTATGAGTC AGATGAAAAC TGTGAAGTGG ACCCCAGCAA ATGTTCATCC 
AGTGAATTAA CAGACCATCA GAGCAACCTG AAAATGTGTT GTGAGCTGGC CTTCTGCAAG 
ATTATCAATT CCTACTGCGT GTTCCCTCGT GAGCTGAAGG AGGTGTTTGC ATCTTGGAAG 
CAGCAGTGCC TGAGCAGGGG CAAGCAGGAC ATCAGCGAGC GGCTGATCAG TGCCTCCCTG 
TTCCTCCGCT TCCTCTGCCC CGCCATCATG TCCCCCAGCC TCTTCAGCCT CATGCAGGAG 
TATCCTGACG ACCGGACATC GCGCACGCTC ACACTCATTG CTAAAGTCAT TCAGAATCTC 
GCCAATTTTG CTAAGTTTGG TAACAAGGAA GAGTACATGG CTTTCATGAA CGACTTTCTG 
GAACATGAGT GGGGGGGAAT GAAGCGATTT CTCCTGGAGA TCTCTAATCC AGATACCATC 
TCAAACATGC CTGGATTTGA GGGCTATATC GACCTGGGCA GAGAGCTCTC AGTTTTGCAT 
TCCCTCTTAT GGGAGGTTGT GTCCCAACTT GATAAGGGTG AAAATTCCTT CCTACAGGCG 
ACCGTGGCAA AACTGGGACC TCTTCCTCGT ATTCTTGCCG ACATCACCAA ATCAATGACC 
AACCCTACGC CAATACAGCA GCAGCTGAGG CGTTTCACTG AGCACAGCTC TAGTCCAAAC 
GTCAGTGGCA GTCTCTCCTC AGGGCTTCAG AAGATATTCG AGGACCCCAC TGATGGTGAC 
TTGCATAAAC TGAAGTCTCC AACCCAGGAA AACATAGATG GCTATTTCAG GGGCAAGACC 
TTGCTGTTGG TTCAGCAGGC GTCCACCCAG AGCATGACTT ACTCAGATAA GGACGAAAGA 
GAGAGCATCT TGCCCAACGG ACGTAGCATC TCCCTCATGG ACCTGCAGGA TCCCCATACC 
GCTCACAGCG ACCACGCTTC CATGATGCAC GACGTGCCTT TGCGCTTGGC CGGCAGCCAG 
CTCTCCATCA CCCAGGTGGC CAACATCAAG CAGCTGTGGG AGACCCAGAG CACGCCCCAA 
AGCGCTCCCC AGGTGAGGAG GCCGCTGCAC CCGGCTTTGA ACCAGCAGGG CAGCCTCCAG 
CCTCTGTCAT TCCAGAACCC GGTGTACCAC CTCAACAACC CACCTGCGCC GTTGCCCAAG 
GCCTCCGTGG ACTCCAGCCT GGAGAACCTG AGCACCGCCA GCTCCCAGAG CCGAAGCAAC 
AGCGAAGACT TCAAGCTCAG CGGGCCCAGC AACAGCAGCA TGGAGGACTT CACCAAGCGC 
AGCACGCAGA GCGAGGACTT CTCTAGGCGG CACACGGTCC CGGACAAGCA CGTCCCCATC 
GCGTTGCCCC GCCAGAACAG CAGCAGCCAG GCACAGATCC GCAAAATGGA CCAGGCCGGG 
TTAGGGGCCA GGGCCAAAGC GCCGCAGTCG CTGCCGCACA GCGCGTCCCT GCGCAGCACG 
GGCAGCATGT CGGCCGTGTC GGGGGCCGTG GTGACTGAGC CCGTCCAGAA CGGCAGCCGC 
TCCCGGCAGC AGTCGTCCTC CTCCAGGGAG AGCCCTGTGC CGAAGGTGAG GGCCATTCAG 
AGGCAGCAAA CGCAGCAGGT CCAGTCTCCT GTGGACTCAG CCACAATGTC ACCAGTGGAA 
AGGACAGCAG CGTGGGTTCT CAATAACGGG CAGTACGAGG AGGTCGAGGA GGACACAGAG 
CAGAACCCAG ATGAAGCCAA GCATGCTGAG AAGTACGAAC AAGAGATAGC AAAGCTGAAG 
GAACGCTTGA AAGTGTCGAG CCGCCGGCTG GAGGAGTACG AGCGGCGGCT CCTGGTGCAG 
GAGCAGCAAA TGCAGAAGCT GCTGATGGAG TACAAGTCCA GGCTGGAGGA CAGCGAGGAG 
AGACTGCGCA GGCAACAGGA GGAGAAGGAC AGCCAGATGA AAAGCATTAT CAGCAGGCTC 
ATGGCAGTTG AAGAGGAACT GAAGAAAGAT CATGCAGAGA TGCAAGCTGT CATCGATGCA 
AAGCAGAAAA TTATTGATGC ACAGGTCATT AATGGGAATG AGATAATTCA AACAGGAGAA 
GCGGATCGTG TCCCTGGACT CTGCCAACAC GCGGCTGATG AGTGCCCTGA CGCAGGTGAA 
GGAGCGCTAC AGCATGCAAG TCCGCAATGG CATCTCCCCC ACCAACCCCA CCAAGCTTTC 
CATCACGGAG AATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015146002.1
CDS	16..3513
Translation	MSLLSPGDVAEWLDSSTEDEKSLLSFLEVPAERSPRRRSISGTSTSEKTNSMDAANTSPFKVSGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNADDRSRGLPKLKESRSHESLLSPGSAVEALDLGAEEKVFVKPLHSSILGQDFCFEVTYSSGSKCFSCSSAAERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYGLPALHSITVHIYKDVEKKKKKDKNNYVGLVNIPMATVTGRQFVEKWYPVSTPTPNKGKSGGPSIRIKSRYQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEEMACAMVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTLATKAIEEYLKLVGQKYLHDALGEFIKALYESDENCEVDPSKCSSSELTDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCLSRGKQDISERLISASLFLRFLCPAIMSPSLFSLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNMPGFEGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVAKLGPLPRILADITKSMTNPTPIQQQLRRFTEHSSSPNVSGSLSSGLQKIFEDPTDGDLHKLKSPTQENIDGYFRGKTLLLVQQASTQSMTYSDKDERESILPNGRSISLMDLQDPHTAHSDHASMMHDVPLRLAGSQLSITQVANIKQLWETQSTPQSAPQVRRPLHPALNQQGSLQPLSFQNPVYHLNNPPAPLPKASVDSSLENLSTASSQSRSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTVPDKHVPIALPRQNSSSQAQIRKMDQAGLGARAKAPQSLPHSASLRSTGSMSAVSGAVVTEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEVEEDTEQNPDEAKHAEKYEQEIAKLKERLKVSSRRLEEYERRLLVQEQQMQKLLMEYKSRLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQVINGNEIIQTGEADRVPGLCQHAADECPDAGEGALQHASPQWHLPHQPHQAFHHGEW

Target ORF information:

RefSeq Version	XM_015290516.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015290516.1

ORF Insert Sequence:

ATGAGTCTCC TTTCTCCCGG CGATGTGGCC GAGTGGCTGG ATAGCAGCAC AGAAGATGAG 
AAAAGCCTTC TGTCGTTTCT CGAGGTCCCG GCAGAGAGAT CTCCTCGCAG ACGGAGCATC 
TCTGGGACCA GCACATCTGA GAAAACCAAC TCCATGGATG CTGCAAATAC CTCTCCTTTC 
AAAGTATCGG GCTTCTTCAG TAAACGTCTG AAAGGGTCCA TCAAGAGGAC AAAGAGTCAG 
TCAAAGCTGG ACAGGAACAC AAGTTTCCGC CTCCCATCCC TCCGCAATGC TGATGACAGG 
TCACGTGGGC TGCCGAAGCT GAAGGAGTCC CGCTCTCATG AGTCCCTGCT GAGCCCCGGC 
AGCGCAGTGG AAGCCCTGGA CCTTGGGGCA GAAGAAAAGG TCTTTGTAAA GCCACTGCAC 
AGCAGCATCC TCGGACAGGA CTTCTGCTTT GAGGTAACCT ACTCCAGCGG CAGTAAATGC 
TTCAGCTGTT CCTCTGCTGC AGAGAGGGAT AAGTGGATGG AAAACCTACG CAGAACAGTG 
CAGCCGAATA AGGACAACTG CCGTCGAGCT GAAAATGTGC TCCGTCTCTG GATCATTGAG 
GCAAAGGACC TGGCCCCCAA GAAGAAATAC TTCTGTGAGC TGTGCCTTGA TGACACTCTC 
TTTGCCCGCA CCACCAGCAA AACGAAAGCA GATAACATTT TCTGGGGGGA GCACTTTGAG 
TTCTACGGCC TCCCAGCTCT TCACAGCATC ACGGTTCACA TCTACAAGGA TGTGGAGAAG 
AAAAAGAAAA AGGACAAAAA CAATTACGTC GGCCTGGTCA ACATTCCCAT GGCCACTGTA 
ACGGGGCGCC AGTTTGTGGA AAAATGGTAC CCAGTCAGCA CACCTACACC CAACAAAGGG 
AAATCAGGAG GCCCTTCCAT TCGCATCAAG TCGCGTTATC AGACCATCAC CATCTTACCC 
ATGGAGCAAT ACAAGGAGTT TGCAGAGTTT GTTACCAGCA ACTACACGAT GCTGTGCTCT 
GTGCTGGAGC CAGTCATCAG CGTAAGGAAT AAGGAAGAGA TGGCTTGTGC AATGGTGCAC 
ATTCTTCAGA GCACGGGGAG AGCCAAGGAC TTCCTAACGG ATCTGGTGAT GTCTGAGGTG 
GATCGCTGCG GGGAACACGA TGTCTTGATC TTCAGGGAGA ACACACTTGC TACCAAAGCT 
ATTGAGGAAT ACCTGAAGTT GGTAGGGCAG AAGTATCTCC ATGATGCGCT AGGGGAGTTT 
ATCAAGGCTT TGTATGAGTC AGATGAAAAC TGTGAAGTGG ACCCCAGCAA ATGTTCATCC 
AGTGAATTAA CAGACCATCA GAGCAACCTG AAAATGTGTT GTGAGCTGGC CTTCTGCAAG 
ATTATCAATT CCTACTGCGT GTTCCCTCGT GAGCTGAAGG AGGTGTTTGC ATCTTGGAAG 
CAGCAGTGCC TGAGCAGGGG CAAGCAGGAC ATCAGCGAGC GGCTGATCAG TGCCTCCCTG 
TTCCTCCGCT TCCTCTGCCC CGCCATCATG TCCCCCAGCC TCTTCAGCCT CATGCAGGAG 
TATCCTGACG ACCGGACATC GCGCACGCTC ACACTCATTG CTAAAGTCAT TCAGAATCTC 
GCCAATTTTG CTAAGTTTGG TAACAAGGAA GAGTACATGG CTTTCATGAA CGACTTTCTG 
GAACATGAGT GGGGGGGAAT GAAGCGATTT CTCCTGGAGA TCTCTAATCC AGATACCATC 
TCAAACATGC CTGGATTTGA GGGCTATATC GACCTGGGCA GAGAGCTCTC AGTTTTGCAT 
TCCCTCTTAT GGGAGGTTGT GTCCCAACTT GATAAGGGTG AAAATTCCTT CCTACAGGCG 
ACCGTGGCAA AACTGGGACC TCTTCCTCGT ATTCTTGCCG ACATCACCAA ATCAATGACC 
AACCCTACGC CAATACAGCA GCAGCTGAGG CGTTTCACTG AGCACAGCTC TAGTCCAAAC 
GTCAGTGGCA GTCTCTCCTC AGGGCTTCAG AAGATATTCG AGGACCCCAC TGATGGTGAC 
TTGCATAAAC TGAAGTCTCC AACCCAGGAA AACATAGATG GCTATTTCAG GGGCAAGACC 
TTGCTGTTGG TTCAGCAGGC GTCCACCCAG AGCATGACTT ACTCAGATAA GGACGAAAGA 
GAGAGCATCT TGCCCAACGG ACGTAGCATC TCCCTCATGG ACCTGCAGGA TCCCCATACC 
GCTCACAGCG ACCACGCTTC CATGATGCAC GACGTGCCTT TGCGCTTGGC CGGCAGCCAG 
CTCTCCATCA CCCAGGTGGC CAACATCAAG CAGCTGTGGG AGACCCAGAG CACGCCCCAA 
AGCGCTCCCC AGGTGAGGAG GCCGCTGCAC CCGGCTTTGA ACCAGCAGGG CAGCCTCCAG 
CCTCTGTCAT TCCAGAACCC GGTGTACCAC CTCAACAACC CACCTGCGCC GTTGCCCAAG 
GCCTCCGTGG ACTCCAGCCT GGAGAACCTG AGCACCGCCA GCTCCCAGAG CCGAAGCAAC 
AGCGAAGACT TCAAGCTCAG CGGGCCCAGC AACAGCAGCA TGGAGGACTT CACCAAGCGC 
AGCACGCAGA GCGAGGACTT CTCTAGGCGG CACACGGTCC CGGACAAGCA CGTCCCCATC 
GCGTTGCCCC GCCAGAACAG CAGCAGCCAG GCACAGATCC GCAAAATGGA CCAGGCCGGG 
TTAGGGGCCA GGGCCAAAGC GCCGCAGTCG CTGCCGCACA GCGCGTCCCT GCGCAGCACG 
GGCAGCATGT CGGCCGTGTC GGGGGCCGTG GTGACTGAGC CCGTCCAGAA CGGCAGCCGC 
TCCCGGCAGC AGTCGTCCTC CTCCAGGGAG AGCCCTGTGC CGAAGGTGAG GGCCATTCAG 
AGGCAGCAAA CGCAGCAGGT CCAGTCTCCT GTGGACTCAG CCACAATGTC ACCAGTGGAA 
AGGACAGCAG CGTGGGTTCT CAATAACGGG CAGTACGAGG AGGTCGAGGA GGACACAGAG 
CAGAACCCAG ATGAAGCCAA GCATGCTGAG AAGTACGAAC AAGAGATAGC AAAGCTGAAG 
GAACGCTTGA AAGTGTCGAG CCGCCGGCTG GAGGAGTACG AGCGGCGGCT CCTGGTGCAG 
GAGCAGCAAA TGCAGAAGCT GCTGATGGAG TACAAGTCCA GGCTGGAGGA CAGCGAGGAG 
AGACTGCGCA GGCAACAGGA GGAGAAGGAC AGCCAGATGA AAAGCATTAT CAGCAGGCTC 
ATGGCAGTTG AAGAGGAACT GAAGAAAGAT CATGCAGAGA TGCAAGCTGT CATCGATGCA 
AAGCAGAAAA TTATTGATGC ACAGGTCATT AATGGGAATG AGATAATTCA AACAGGAGAA 
GCGGATCGTG TCCCTGGACT CTGCCAACAC GCGGCTGATG AGTGCCCTGA CGCAGGTGAA 
GGAGCGCTAC AGCATGCAAG TCCGCAATGG CATCTCCCCC ACCAACCCCA CCAAGCTTTC 
CATCACGGAG AATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_004943254.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004943311.2 Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X11, mRNA.

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CloneID	OGa39049
Clone ID Related Accession (Same CDS sequence)	XM_004943254.2
Accession Version	XM_004943254.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	ras GTPase-activating protein nGAP isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004943254.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAGCA CTTGTGACAA AGAGGTCCCG GCAGAGAGAT CTCCTCGCAG ACGGAGCATC 
TCTGGGACCA GCACATCTGA GAAAACCAAC TCCATGGATG CTGCAAATAC CTCTCCTTTC 
AAAGTATCGG GCTTCTTCAG TAAACGTCTG AAAGGGTCCA TCAAGAGGAC AAAGAGTCAG 
TCAAAGCTGG ACAGGAACAC AAGTTTCCGC CTCCCATCCC TCCGCAATGC TGATGACAGG 
TCACGTGGGC TGCCGAAGCT GAAGGAGTCC CGCTCTCATG AGTCCCTGCT GAGCCCCGGC 
AGCGCAGTGG AAGCCCTGGA CCTTGGGGCA GAAGAAAAGG TCTTTGTAAA GCCACTGCAC 
AGCAGCATCC TCGGACAGGA CTTCTGCTTT GAGGTAACCT ACTCCAGCGG CAGTAAATGC 
TTCAGCTGTT CCTCTGCTGC AGAGAGGGAT AAGTGGATGG AAAACCTACG CAGAACAGTG 
CAGCCGAATA AGGACAACTG CCGTCGAGCT GAAAATGTGC TCCGTCTCTG GATCATTGAG 
GCAAAGGACC TGGCCCCCAA GAAGAAATAC TTCTGTGAGC TGTGCCTTGA TGACACTCTC 
TTTGCCCGCA CCACCAGCAA AACGAAAGCA GATAACATTT TCTGGGGGGA GCACTTTGAG 
TTCTACGGCC TCCCAGCTCT TCACAGCATC ACGGTTCACA TCTACAAGGA TGTGGAGAAG 
AAAAAGAAAA AGGACAAAAA CAATTACGTC GGCCTGGTCA ACATTCCCAT GGCCACTGTA 
ACGGGGCGCC AGTTTGTGGA AAAATGGTAC CCAGTCAGCA CACCTACACC CAACAAAGGG 
AAATCAGGAG GCCCTTCCAT TCGCATCAAG TCGCGTTATC AGACCATCAC CATCTTACCC 
ATGGAGCAAT ACAAGGAGTT TGCAGAGTTT GTTACCAGCA ACTACACGAT GCTGTGCTCT 
GTGCTGGAGC CAGTCATCAG CGTAAGGAAT AAGGAAGAGA TGGCTTGTGC AATGGTGCAC 
ATTCTTCAGA GCACGGGGAG AGCCAAGGAC TTCCTAACGG ATCTGGTGAT GTCTGAGGTG 
GATCGCTGCG GGGAACACGA TGTCTTGATC TTCAGGGAGA ACACACTTGC TACCAAAGCT 
ATTGAGGAAT ACCTGAAGTT GGTAGGGCAG AAGTATCTCC ATGATGCGCT AGGGGAGTTT 
ATCAAGGCTT TGTATGAGTC AGATGAAAAC TGTGAAGTGG ACCCCAGCAA ATGTTCATCC 
AGTGAATTAA CAGACCATCA GAGCAACCTG AAAATGTGTT GTGAGCTGGC CTTCTGCAAG 
ATTATCAATT CCTACTGCGT GTTCCCTCGT GAGCTGAAGG AGGTGTTTGC ATCTTGGAAG 
CAGCAGTGCC TGAGCAGGGG CAAGCAGGAC ATCAGCGAGC GGCTGATCAG TGCCTCCCTG 
TTCCTCCGCT TCCTCTGCCC CGCCATCATG TCCCCCAGCC TCTTCAGCCT CATGCAGGAG 
TATCCTGACG ACCGGACATC GCGCACGCTC ACACTCATTG CTAAAGTCAT TCAGAATCTC 
GCCAATTTTG CTAAGTTTGG TAACAAGGAA GAGTACATGG CTTTCATGAA CGACTTTCTG 
GAACATGAGT GGGGGGGAAT GAAGCGATTT CTCCTGGAGA TCTCTAATCC AGATACCATC 
TCAAACATGC CTGGATTTGA GGGCTATATC GACCTGGGCA GAGAGCTCTC AGTTTTGCAT 
TCCCTCTTAT GGGAGGTTGT GTCCCAACTT GATAAGGGTG AAAATTCCTT CCTACAGGCG 
ACCGTGGCAA AACTGGGACC TCTTCCTCGT ATTCTTGCCG ACATCACCAA ATCAATGACC 
AACCCTACGC CAATACAGCA GCAGCTGAGG CGTTTCACTG AGCACAGCTC TAGTCCAAAC 
GTCAGTGGCA GTCTCTCCTC AGGGCTTCAG AAGATATTCG AGGACCCCAC TGATGGTGAC 
TTGCATAAAC TGAAGTCTCC AACCCAGGAA AACATAGATG GCTATTTCAG GGGCAAGACC 
TTGCTGTTGG TTCAGCAGGC GTCCACCCAG AGCATGACTT ACTCAGATAA GGACGAAAGA 
GAGAGCATCT TGCCCAACGG ACGTAGCATC TCCCTCATGG ACCTGCAGGA TCCCCATACC 
GCTCACAGCG ACCACGCTTC CATGATGCAC GACGTGCCTT TGCGCTTGGC CGGCAGCCAG 
CTCTCCATCA CCCAGGTGGC CAACATCAAG CAGCTGTGGG AGACCCAGAG CACGCCCCAA 
AGCGCTCCCC AGGTGAGGAG GCCGCTGCAC CCGGCTTTGA ACCAGCAGGG CAGCCTCCAG 
CCTCTGTCAT TCCAGAACCC GGTGTACCAC CTCAACAACC CACCTGCGCC GTTGCCCAAG 
GCCTCCGTGG ACTCCAGCCT GGAGAACCTG AGCACCGCCA GCTCCCAGAG CCGAAGCAAC 
AGCGAAGACT TCAAGCTCAG CGGGCCCAGC AACAGCAGCA TGGAGGACTT CACCAAGCGC 
AGCACGCAGA GCGAGGACTT CTCTAGGCGG CACACGGTCC CGGACAAGCA CGTCCCCATC 
GCGTTGCCCC GCCAGAACAG CAGCAGCCAG GCACAGATCC GCAAAATGGA CCAGGCCGGG 
TTAGGGGCCA GGGCCAAAGC GCCGCAGTCG CTGCCGCACA GCGCGTCCCT GCGCAGCACG 
GGCAGCATGT CGGCCGTGTC GGGGGCCGTG GTGACTGAGC CCGTCCAGAA CGGCAGCCGC 
TCCCGGCAGC AGTCGTCCTC CTCCAGGGAG AGCCCTGTGC CGAAGGTGAG GGCCATTCAG 
AGGCAGCAAA CGCAGCAGGT CCAGTCTCCT GTGGACTCAG CCACAATGTC ACCAGTGGAA 
AGGACAGCAG CGTGGGTTCT CAATAACGGG CAGTACGAGG AGGTCGAGGA GGACACAGAG 
CAGAACCCAG ATGAAGCCAA GCATGCTGAG AAGTACGAAC AAGAGATAGC AAAGCTGAAG 
GAACGCTTGA AAGTGTCGAG CCGCCGGCTG GAGGAGTACG AGCGGCGGCT CCTGGTGCAG 
GAGCAGCAAA TGCAGAAGCT GCTGATGGAG TACAAGTCCA GGCTGGAGGA CAGCGAGGAG 
AGACTGCGCA GGCAACAGGA GGAGAAGGAC AGCCAGATGA AAAGCATTAT CAGCAGGCTC 
ATGGCAGTTG AAGAGGAACT GAAGAAAGAT CATGCAGAGA TGCAAGCTGT CATCGATGCA 
AAGCAGAAAA TTATTGATGC ACAGGTCATT AATGGGAATG AGATAATTCA AACAGGAGAA 
GCGGATCGTG TCCCTGGACT CTGCCAACAC GCGGCTGATG AGTGCCCTGA CGCAGGTGAA 
GGAGCGCTAC AGCATGCAAG TCCGCAATGG CATCTCCCCC ACCAACCCCA CCAAGCTTTC 
CATCACGGAG AATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004943311.2
CDS	694..4131
Translation	MGSTCDKEVPAERSPRRRSISGTSTSEKTNSMDAANTSPFKVSGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNADDRSRGLPKLKESRSHESLLSPGSAVEALDLGAEEKVFVKPLHSSILGQDFCFEVTYSSGSKCFSCSSAAERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYGLPALHSITVHIYKDVEKKKKKDKNNYVGLVNIPMATVTGRQFVEKWYPVSTPTPNKGKSGGPSIRIKSRYQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEEMACAMVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTLATKAIEEYLKLVGQKYLHDALGEFIKALYESDENCEVDPSKCSSSELTDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCLSRGKQDISERLISASLFLRFLCPAIMSPSLFSLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNMPGFEGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVAKLGPLPRILADITKSMTNPTPIQQQLRRFTEHSSSPNVSGSLSSGLQKIFEDPTDGDLHKLKSPTQENIDGYFRGKTLLLVQQASTQSMTYSDKDERESILPNGRSISLMDLQDPHTAHSDHASMMHDVPLRLAGSQLSITQVANIKQLWETQSTPQSAPQVRRPLHPALNQQGSLQPLSFQNPVYHLNNPPAPLPKASVDSSLENLSTASSQSRSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTVPDKHVPIALPRQNSSSQAQIRKMDQAGLGARAKAPQSLPHSASLRSTGSMSAVSGAVVTEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEVEEDTEQNPDEAKHAEKYEQEIAKLKERLKVSSRRLEEYERRLLVQEQQMQKLLMEYKSRLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQVINGNEIIQTGEADRVPGLCQHAADECPDAGEGALQHASPQWHLPHQPHQAFHHGEW

Target ORF information:

RefSeq Version	XM_004943254.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004943254.2

ORF Insert Sequence:

ATGGGCAGCA CTTGTGACAA AGAGGTCCCG GCAGAGAGAT CTCCTCGCAG ACGGAGCATC 
TCTGGGACCA GCACATCTGA GAAAACCAAC TCCATGGATG CTGCAAATAC CTCTCCTTTC 
AAAGTATCGG GCTTCTTCAG TAAACGTCTG AAAGGGTCCA TCAAGAGGAC AAAGAGTCAG 
TCAAAGCTGG ACAGGAACAC AAGTTTCCGC CTCCCATCCC TCCGCAATGC TGATGACAGG 
TCACGTGGGC TGCCGAAGCT GAAGGAGTCC CGCTCTCATG AGTCCCTGCT GAGCCCCGGC 
AGCGCAGTGG AAGCCCTGGA CCTTGGGGCA GAAGAAAAGG TCTTTGTAAA GCCACTGCAC 
AGCAGCATCC TCGGACAGGA CTTCTGCTTT GAGGTAACCT ACTCCAGCGG CAGTAAATGC 
TTCAGCTGTT CCTCTGCTGC AGAGAGGGAT AAGTGGATGG AAAACCTACG CAGAACAGTG 
CAGCCGAATA AGGACAACTG CCGTCGAGCT GAAAATGTGC TCCGTCTCTG GATCATTGAG 
GCAAAGGACC TGGCCCCCAA GAAGAAATAC TTCTGTGAGC TGTGCCTTGA TGACACTCTC 
TTTGCCCGCA CCACCAGCAA AACGAAAGCA GATAACATTT TCTGGGGGGA GCACTTTGAG 
TTCTACGGCC TCCCAGCTCT TCACAGCATC ACGGTTCACA TCTACAAGGA TGTGGAGAAG 
AAAAAGAAAA AGGACAAAAA CAATTACGTC GGCCTGGTCA ACATTCCCAT GGCCACTGTA 
ACGGGGCGCC AGTTTGTGGA AAAATGGTAC CCAGTCAGCA CACCTACACC CAACAAAGGG 
AAATCAGGAG GCCCTTCCAT TCGCATCAAG TCGCGTTATC AGACCATCAC CATCTTACCC 
ATGGAGCAAT ACAAGGAGTT TGCAGAGTTT GTTACCAGCA ACTACACGAT GCTGTGCTCT 
GTGCTGGAGC CAGTCATCAG CGTAAGGAAT AAGGAAGAGA TGGCTTGTGC AATGGTGCAC 
ATTCTTCAGA GCACGGGGAG AGCCAAGGAC TTCCTAACGG ATCTGGTGAT GTCTGAGGTG 
GATCGCTGCG GGGAACACGA TGTCTTGATC TTCAGGGAGA ACACACTTGC TACCAAAGCT 
ATTGAGGAAT ACCTGAAGTT GGTAGGGCAG AAGTATCTCC ATGATGCGCT AGGGGAGTTT 
ATCAAGGCTT TGTATGAGTC AGATGAAAAC TGTGAAGTGG ACCCCAGCAA ATGTTCATCC 
AGTGAATTAA CAGACCATCA GAGCAACCTG AAAATGTGTT GTGAGCTGGC CTTCTGCAAG 
ATTATCAATT CCTACTGCGT GTTCCCTCGT GAGCTGAAGG AGGTGTTTGC ATCTTGGAAG 
CAGCAGTGCC TGAGCAGGGG CAAGCAGGAC ATCAGCGAGC GGCTGATCAG TGCCTCCCTG 
TTCCTCCGCT TCCTCTGCCC CGCCATCATG TCCCCCAGCC TCTTCAGCCT CATGCAGGAG 
TATCCTGACG ACCGGACATC GCGCACGCTC ACACTCATTG CTAAAGTCAT TCAGAATCTC 
GCCAATTTTG CTAAGTTTGG TAACAAGGAA GAGTACATGG CTTTCATGAA CGACTTTCTG 
GAACATGAGT GGGGGGGAAT GAAGCGATTT CTCCTGGAGA TCTCTAATCC AGATACCATC 
TCAAACATGC CTGGATTTGA GGGCTATATC GACCTGGGCA GAGAGCTCTC AGTTTTGCAT 
TCCCTCTTAT GGGAGGTTGT GTCCCAACTT GATAAGGGTG AAAATTCCTT CCTACAGGCG 
ACCGTGGCAA AACTGGGACC TCTTCCTCGT ATTCTTGCCG ACATCACCAA ATCAATGACC 
AACCCTACGC CAATACAGCA GCAGCTGAGG CGTTTCACTG AGCACAGCTC TAGTCCAAAC 
GTCAGTGGCA GTCTCTCCTC AGGGCTTCAG AAGATATTCG AGGACCCCAC TGATGGTGAC 
TTGCATAAAC TGAAGTCTCC AACCCAGGAA AACATAGATG GCTATTTCAG GGGCAAGACC 
TTGCTGTTGG TTCAGCAGGC GTCCACCCAG AGCATGACTT ACTCAGATAA GGACGAAAGA 
GAGAGCATCT TGCCCAACGG ACGTAGCATC TCCCTCATGG ACCTGCAGGA TCCCCATACC 
GCTCACAGCG ACCACGCTTC CATGATGCAC GACGTGCCTT TGCGCTTGGC CGGCAGCCAG 
CTCTCCATCA CCCAGGTGGC CAACATCAAG CAGCTGTGGG AGACCCAGAG CACGCCCCAA 
AGCGCTCCCC AGGTGAGGAG GCCGCTGCAC CCGGCTTTGA ACCAGCAGGG CAGCCTCCAG 
CCTCTGTCAT TCCAGAACCC GGTGTACCAC CTCAACAACC CACCTGCGCC GTTGCCCAAG 
GCCTCCGTGG ACTCCAGCCT GGAGAACCTG AGCACCGCCA GCTCCCAGAG CCGAAGCAAC 
AGCGAAGACT TCAAGCTCAG CGGGCCCAGC AACAGCAGCA TGGAGGACTT CACCAAGCGC 
AGCACGCAGA GCGAGGACTT CTCTAGGCGG CACACGGTCC CGGACAAGCA CGTCCCCATC 
GCGTTGCCCC GCCAGAACAG CAGCAGCCAG GCACAGATCC GCAAAATGGA CCAGGCCGGG 
TTAGGGGCCA GGGCCAAAGC GCCGCAGTCG CTGCCGCACA GCGCGTCCCT GCGCAGCACG 
GGCAGCATGT CGGCCGTGTC GGGGGCCGTG GTGACTGAGC CCGTCCAGAA CGGCAGCCGC 
TCCCGGCAGC AGTCGTCCTC CTCCAGGGAG AGCCCTGTGC CGAAGGTGAG GGCCATTCAG 
AGGCAGCAAA CGCAGCAGGT CCAGTCTCCT GTGGACTCAG CCACAATGTC ACCAGTGGAA 
AGGACAGCAG CGTGGGTTCT CAATAACGGG CAGTACGAGG AGGTCGAGGA GGACACAGAG 
CAGAACCCAG ATGAAGCCAA GCATGCTGAG AAGTACGAAC AAGAGATAGC AAAGCTGAAG 
GAACGCTTGA AAGTGTCGAG CCGCCGGCTG GAGGAGTACG AGCGGCGGCT CCTGGTGCAG 
GAGCAGCAAA TGCAGAAGCT GCTGATGGAG TACAAGTCCA GGCTGGAGGA CAGCGAGGAG 
AGACTGCGCA GGCAACAGGA GGAGAAGGAC AGCCAGATGA AAAGCATTAT CAGCAGGCTC 
ATGGCAGTTG AAGAGGAACT GAAGAAAGAT CATGCAGAGA TGCAAGCTGT CATCGATGCA 
AAGCAGAAAA TTATTGATGC ACAGGTCATT AATGGGAATG AGATAATTCA AACAGGAGAA 
GCGGATCGTG TCCCTGGACT CTGCCAACAC GCGGCTGATG AGTGCCCTGA CGCAGGTGAA 
GGAGCGCTAC AGCATGCAAG TCCGCAATGG CATCTCCCCC ACCAACCCCA CCAAGCTTTC 
CATCACGGAG AATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_015290519.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015146005.1 Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OGa39050
Clone ID Related Accession (Same CDS sequence)	XM_015290519.1
Accession Version	XM_015290519.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3429bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	ras GTPase-activating protein nGAP isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCTCG CAGAGGTCCC GGCAGAGAGA TCTCCTCGCA GACGGAGCAT CTCTGGGACC 
AGCACATCTG AGAAAACCAA CTCCATGGAT GCTGCAAATA CCTCTCCTTT CAAAGTATCG 
GGCTTCTTCA GTAAACGTCT GAAAGGGTCC ATCAAGAGGA CAAAGAGTCA GTCAAAGCTG 
GACAGGAACA CAAGTTTCCG CCTCCCATCC CTCCGCAATG CTGATGACAG GTCACGTGGG 
CTGCCGAAGC TGAAGGAGTC CCGCTCTCAT GAGTCCCTGC TGAGCCCCGG CAGCGCAGTG 
GAAGCCCTGG ACCTTGGGGC AGAAGAAAAG GTCTTTGTAA AGCCACTGCA CAGCAGCATC 
CTCGGACAGG ACTTCTGCTT TGAGGTAACC TACTCCAGCG GCAGTAAATG CTTCAGCTGT 
TCCTCTGCTG CAGAGAGGGA TAAGTGGATG GAAAACCTAC GCAGAACAGT GCAGCCGAAT 
AAGGACAACT GCCGTCGAGC TGAAAATGTG CTCCGTCTCT GGATCATTGA GGCAAAGGAC 
CTGGCCCCCA AGAAGAAATA CTTCTGTGAG CTGTGCCTTG ATGACACTCT CTTTGCCCGC 
ACCACCAGCA AAACGAAAGC AGATAACATT TTCTGGGGGG AGCACTTTGA GTTCTACGGC 
CTCCCAGCTC TTCACAGCAT CACGGTTCAC ATCTACAAGG ATGTGGAGAA GAAAAAGAAA 
AAGGACAAAA ACAATTACGT CGGCCTGGTC AACATTCCCA TGGCCACTGT AACGGGGCGC 
CAGTTTGTGG AAAAATGGTA CCCAGTCAGC ACACCTACAC CCAACAAAGG GAAATCAGGA 
GGCCCTTCCA TTCGCATCAA GTCGCGTTAT CAGACCATCA CCATCTTACC CATGGAGCAA 
TACAAGGAGT TTGCAGAGTT TGTTACCAGC AACTACACGA TGCTGTGCTC TGTGCTGGAG 
CCAGTCATCA GCGTAAGGAA TAAGGAAGAG ATGGCTTGTG CAATGGTGCA CATTCTTCAG 
AGCACGGGGA GAGCCAAGGA CTTCCTAACG GATCTGGTGA TGTCTGAGGT GGATCGCTGC 
GGGGAACACG ATGTCTTGAT CTTCAGGGAG AACACACTTG CTACCAAAGC TATTGAGGAA 
TACCTGAAGT TGGTAGGGCA GAAGTATCTC CATGATGCGC TAGGGGAGTT TATCAAGGCT 
TTGTATGAGT CAGATGAAAA CTGTGAAGTG GACCCCAGCA AATGTTCATC CAGTGAATTA 
ACAGACCATC AGAGCAACCT GAAAATGTGT TGTGAGCTGG CCTTCTGCAA GATTATCAAT 
TCCTACTGCG TGTTCCCTCG TGAGCTGAAG GAGGTGTTTG CATCTTGGAA GCAGCAGTGC 
CTGAGCAGGG GCAAGCAGGA CATCAGCGAG CGGCTGATCA GTGCCTCCCT GTTCCTCCGC 
TTCCTCTGCC CCGCCATCAT GTCCCCCAGC CTCTTCAGCC TCATGCAGGA GTATCCTGAC 
GACCGGACAT CGCGCACGCT CACACTCATT GCTAAAGTCA TTCAGAATCT CGCCAATTTT 
GCTAAGTTTG GTAACAAGGA AGAGTACATG GCTTTCATGA ACGACTTTCT GGAACATGAG 
TGGGGGGGAA TGAAGCGATT TCTCCTGGAG ATCTCTAATC CAGATACCAT CTCAAACATG 
CCTGGATTTG AGGGCTATAT CGACCTGGGC AGAGAGCTCT CAGTTTTGCA TTCCCTCTTA 
TGGGAGGTTG TGTCCCAACT TGATAAGGGT GAAAATTCCT TCCTACAGGC GACCGTGGCA 
AAACTGGGAC CTCTTCCTCG TATTCTTGCC GACATCACCA AATCAATGAC CAACCCTACG 
CCAATACAGC AGCAGCTGAG GCGTTTCACT GAGCACAGCT CTAGTCCAAA CGTCAGTGGC 
AGTCTCTCCT CAGGGCTTCA GAAGATATTC GAGGACCCCA CTGATGGTGA CTTGCATAAA 
CTGAAGTCTC CAACCCAGGA AAACATAGAT GGCTATTTCA GGGGCAAGAC CTTGCTGTTG 
GTTCAGCAGG CGTCCACCCA GAGCATGACT TACTCAGATA AGGACGAAAG AGAGAGCATC 
TTGCCCAACG GACGTAGCAT CTCCCTCATG GACCTGCAGG ATCCCCATAC CGCTCACAGC 
GACCACGCTT CCATGATGCA CGACGTGCCT TTGCGCTTGG CCGGCAGCCA GCTCTCCATC 
ACCCAGGTGG CCAACATCAA GCAGCTGTGG GAGACCCAGA GCACGCCCCA AAGCGCTCCC 
CAGGTGAGGA GGCCGCTGCA CCCGGCTTTG AACCAGCAGG GCAGCCTCCA GCCTCTGTCA 
TTCCAGAACC CGGTGTACCA CCTCAACAAC CCACCTGCGC CGTTGCCCAA GGCCTCCGTG 
GACTCCAGCC TGGAGAACCT GAGCACCGCC AGCTCCCAGA GCCGAAGCAA CAGCGAAGAC 
TTCAAGCTCA GCGGGCCCAG CAACAGCAGC ATGGAGGACT TCACCAAGCG CAGCACGCAG 
AGCGAGGACT TCTCTAGGCG GCACACGGTC CCGGACAAGC ACGTCCCCAT CGCGTTGCCC 
CGCCAGAACA GCAGCAGCCA GGCACAGATC CGCAAAATGG ACCAGGCCGG GTTAGGGGCC 
AGGGCCAAAG CGCCGCAGTC GCTGCCGCAC AGCGCGTCCC TGCGCAGCAC GGGCAGCATG 
TCGGCCGTGT CGGGGGCCGT GGTGACTGAG CCCGTCCAGA ACGGCAGCCG CTCCCGGCAG 
CAGTCGTCCT CCTCCAGGGA GAGCCCTGTG CCGAAGGTGA GGGCCATTCA GAGGCAGCAA 
ACGCAGCAGG TCCAGTCTCC TGTGGACTCA GCCACAATGT CACCAGTGGA AAGGACAGCA 
GCGTGGGTTC TCAATAACGG GCAGTACGAG GAGGTCGAGG AGGACACAGA GCAGAACCCA 
GATGAAGCCA AGCATGCTGA GAAGTACGAA CAAGAGATAG CAAAGCTGAA GGAACGCTTG 
AAAGTGTCGA GCCGCCGGCT GGAGGAGTAC GAGCGGCGGC TCCTGGTGCA GGAGCAGCAA 
ATGCAGAAGC TGCTGATGGA GTACAAGTCC AGGCTGGAGG ACAGCGAGGA GAGACTGCGC 
AGGCAACAGG AGGAGAAGGA CAGCCAGATG AAAAGCATTA TCAGCAGGCT CATGGCAGTT 
GAAGAGGAAC TGAAGAAAGA TCATGCAGAG ATGCAAGCTG TCATCGATGC AAAGCAGAAA 
ATTATTGATG CACAGGTCAT TAATGGGAAT GAGATAATTC AAACAGGAGA AGCGGATCGT 
GTCCCTGGAC TCTGCCAACA CGCGGCTGAT GAGTGCCCTG ACGCAGGTGA AGGAGCGCTA 
CAGCATGCAA GTCCGCAATG GCATCTCCCC CACCAACCCC ACCAAGCTTT CCATCACGGA 
GAATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015146005.1
CDS	237..3665
Translation	MQLAEVPAERSPRRRSISGTSTSEKTNSMDAANTSPFKVSGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNADDRSRGLPKLKESRSHESLLSPGSAVEALDLGAEEKVFVKPLHSSILGQDFCFEVTYSSGSKCFSCSSAAERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYGLPALHSITVHIYKDVEKKKKKDKNNYVGLVNIPMATVTGRQFVEKWYPVSTPTPNKGKSGGPSIRIKSRYQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEEMACAMVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTLATKAIEEYLKLVGQKYLHDALGEFIKALYESDENCEVDPSKCSSSELTDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCLSRGKQDISERLISASLFLRFLCPAIMSPSLFSLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNMPGFEGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVAKLGPLPRILADITKSMTNPTPIQQQLRRFTEHSSSPNVSGSLSSGLQKIFEDPTDGDLHKLKSPTQENIDGYFRGKTLLLVQQASTQSMTYSDKDERESILPNGRSISLMDLQDPHTAHSDHASMMHDVPLRLAGSQLSITQVANIKQLWETQSTPQSAPQVRRPLHPALNQQGSLQPLSFQNPVYHLNNPPAPLPKASVDSSLENLSTASSQSRSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTVPDKHVPIALPRQNSSSQAQIRKMDQAGLGARAKAPQSLPHSASLRSTGSMSAVSGAVVTEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEVEEDTEQNPDEAKHAEKYEQEIAKLKERLKVSSRRLEEYERRLLVQEQQMQKLLMEYKSRLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQVINGNEIIQTGEADRVPGLCQHAADECPDAGEGALQHASPQWHLPHQPHQAFHHGEW

Target ORF information:

RefSeq Version	XM_015290519.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015290519.1

ORF Insert Sequence:

ATGCAGCTCG CAGAGGTCCC GGCAGAGAGA TCTCCTCGCA GACGGAGCAT CTCTGGGACC 
AGCACATCTG AGAAAACCAA CTCCATGGAT GCTGCAAATA CCTCTCCTTT CAAAGTATCG 
GGCTTCTTCA GTAAACGTCT GAAAGGGTCC ATCAAGAGGA CAAAGAGTCA GTCAAAGCTG 
GACAGGAACA CAAGTTTCCG CCTCCCATCC CTCCGCAATG CTGATGACAG GTCACGTGGG 
CTGCCGAAGC TGAAGGAGTC CCGCTCTCAT GAGTCCCTGC TGAGCCCCGG CAGCGCAGTG 
GAAGCCCTGG ACCTTGGGGC AGAAGAAAAG GTCTTTGTAA AGCCACTGCA CAGCAGCATC 
CTCGGACAGG ACTTCTGCTT TGAGGTAACC TACTCCAGCG GCAGTAAATG CTTCAGCTGT 
TCCTCTGCTG CAGAGAGGGA TAAGTGGATG GAAAACCTAC GCAGAACAGT GCAGCCGAAT 
AAGGACAACT GCCGTCGAGC TGAAAATGTG CTCCGTCTCT GGATCATTGA GGCAAAGGAC 
CTGGCCCCCA AGAAGAAATA CTTCTGTGAG CTGTGCCTTG ATGACACTCT CTTTGCCCGC 
ACCACCAGCA AAACGAAAGC AGATAACATT TTCTGGGGGG AGCACTTTGA GTTCTACGGC 
CTCCCAGCTC TTCACAGCAT CACGGTTCAC ATCTACAAGG ATGTGGAGAA GAAAAAGAAA 
AAGGACAAAA ACAATTACGT CGGCCTGGTC AACATTCCCA TGGCCACTGT AACGGGGCGC 
CAGTTTGTGG AAAAATGGTA CCCAGTCAGC ACACCTACAC CCAACAAAGG GAAATCAGGA 
GGCCCTTCCA TTCGCATCAA GTCGCGTTAT CAGACCATCA CCATCTTACC CATGGAGCAA 
TACAAGGAGT TTGCAGAGTT TGTTACCAGC AACTACACGA TGCTGTGCTC TGTGCTGGAG 
CCAGTCATCA GCGTAAGGAA TAAGGAAGAG ATGGCTTGTG CAATGGTGCA CATTCTTCAG 
AGCACGGGGA GAGCCAAGGA CTTCCTAACG GATCTGGTGA TGTCTGAGGT GGATCGCTGC 
GGGGAACACG ATGTCTTGAT CTTCAGGGAG AACACACTTG CTACCAAAGC TATTGAGGAA 
TACCTGAAGT TGGTAGGGCA GAAGTATCTC CATGATGCGC TAGGGGAGTT TATCAAGGCT 
TTGTATGAGT CAGATGAAAA CTGTGAAGTG GACCCCAGCA AATGTTCATC CAGTGAATTA 
ACAGACCATC AGAGCAACCT GAAAATGTGT TGTGAGCTGG CCTTCTGCAA GATTATCAAT 
TCCTACTGCG TGTTCCCTCG TGAGCTGAAG GAGGTGTTTG CATCTTGGAA GCAGCAGTGC 
CTGAGCAGGG GCAAGCAGGA CATCAGCGAG CGGCTGATCA GTGCCTCCCT GTTCCTCCGC 
TTCCTCTGCC CCGCCATCAT GTCCCCCAGC CTCTTCAGCC TCATGCAGGA GTATCCTGAC 
GACCGGACAT CGCGCACGCT CACACTCATT GCTAAAGTCA TTCAGAATCT CGCCAATTTT 
GCTAAGTTTG GTAACAAGGA AGAGTACATG GCTTTCATGA ACGACTTTCT GGAACATGAG 
TGGGGGGGAA TGAAGCGATT TCTCCTGGAG ATCTCTAATC CAGATACCAT CTCAAACATG 
CCTGGATTTG AGGGCTATAT CGACCTGGGC AGAGAGCTCT CAGTTTTGCA TTCCCTCTTA 
TGGGAGGTTG TGTCCCAACT TGATAAGGGT GAAAATTCCT TCCTACAGGC GACCGTGGCA 
AAACTGGGAC CTCTTCCTCG TATTCTTGCC GACATCACCA AATCAATGAC CAACCCTACG 
CCAATACAGC AGCAGCTGAG GCGTTTCACT GAGCACAGCT CTAGTCCAAA CGTCAGTGGC 
AGTCTCTCCT CAGGGCTTCA GAAGATATTC GAGGACCCCA CTGATGGTGA CTTGCATAAA 
CTGAAGTCTC CAACCCAGGA AAACATAGAT GGCTATTTCA GGGGCAAGAC CTTGCTGTTG 
GTTCAGCAGG CGTCCACCCA GAGCATGACT TACTCAGATA AGGACGAAAG AGAGAGCATC 
TTGCCCAACG GACGTAGCAT CTCCCTCATG GACCTGCAGG ATCCCCATAC CGCTCACAGC 
GACCACGCTT CCATGATGCA CGACGTGCCT TTGCGCTTGG CCGGCAGCCA GCTCTCCATC 
ACCCAGGTGG CCAACATCAA GCAGCTGTGG GAGACCCAGA GCACGCCCCA AAGCGCTCCC 
CAGGTGAGGA GGCCGCTGCA CCCGGCTTTG AACCAGCAGG GCAGCCTCCA GCCTCTGTCA 
TTCCAGAACC CGGTGTACCA CCTCAACAAC CCACCTGCGC CGTTGCCCAA GGCCTCCGTG 
GACTCCAGCC TGGAGAACCT GAGCACCGCC AGCTCCCAGA GCCGAAGCAA CAGCGAAGAC 
TTCAAGCTCA GCGGGCCCAG CAACAGCAGC ATGGAGGACT TCACCAAGCG CAGCACGCAG 
AGCGAGGACT TCTCTAGGCG GCACACGGTC CCGGACAAGC ACGTCCCCAT CGCGTTGCCC 
CGCCAGAACA GCAGCAGCCA GGCACAGATC CGCAAAATGG ACCAGGCCGG GTTAGGGGCC 
AGGGCCAAAG CGCCGCAGTC GCTGCCGCAC AGCGCGTCCC TGCGCAGCAC GGGCAGCATG 
TCGGCCGTGT CGGGGGCCGT GGTGACTGAG CCCGTCCAGA ACGGCAGCCG CTCCCGGCAG 
CAGTCGTCCT CCTCCAGGGA GAGCCCTGTG CCGAAGGTGA GGGCCATTCA GAGGCAGCAA 
ACGCAGCAGG TCCAGTCTCC TGTGGACTCA GCCACAATGT CACCAGTGGA AAGGACAGCA 
GCGTGGGTTC TCAATAACGG GCAGTACGAG GAGGTCGAGG AGGACACAGA GCAGAACCCA 
GATGAAGCCA AGCATGCTGA GAAGTACGAA CAAGAGATAG CAAAGCTGAA GGAACGCTTG 
AAAGTGTCGA GCCGCCGGCT GGAGGAGTAC GAGCGGCGGC TCCTGGTGCA GGAGCAGCAA 
ATGCAGAAGC TGCTGATGGA GTACAAGTCC AGGCTGGAGG ACAGCGAGGA GAGACTGCGC 
AGGCAACAGG AGGAGAAGGA CAGCCAGATG AAAAGCATTA TCAGCAGGCT CATGGCAGTT 
GAAGAGGAAC TGAAGAAAGA TCATGCAGAG ATGCAAGCTG TCATCGATGC AAAGCAGAAA 
ATTATTGATG CACAGGTCAT TAATGGGAAT GAGATAATTC AAACAGGAGA AGCGGATCGT 
GTCCCTGGAC TCTGCCAACA CGCGGCTGAT GAGTGCCCTG ACGCAGGTGA AGGAGCGCTA 
CAGCATGCAA GTCCGCAATG GCATCTCCCC CACCAACCCC ACCAAGCTTT CCATCACGGA 
GAATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_015290520.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015146006.1 Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X13, mRNA.

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CloneID	OGa39051
Clone ID Related Accession (Same CDS sequence)	XM_015290520.1
Accession Version	XM_015290520.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	ras GTPase-activating protein nGAP isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCCGC CAAGCTGGGG CTTCTTCAGT AAACGTCTGA AAGGGTCCAT CAAGAGGACA 
AAGAGTCAGT CAAAGCTGGA CAGGAACACA AGTTTCCGCC TCCCATCCCT CCGCAATGCT 
GATGACAGGT CACGTGGGCT GCCGAAGCTG AAGGAGTCCC GCTCTCATGA GTCCCTGCTG 
AGCCCCGGCA GCGCAGTGGA AGCCCTGGAC CTTGGGGCAG AAGAAAAGGT CTTTGTAAAG 
CCACTGCACA GCAGCATCCT CGGACAGGAC TTCTGCTTTG AGGTAACCTA CTCCAGCGGC 
AGTAAATGCT TCAGCTGTTC CTCTGCTGCA GAGAGGGATA AGTGGATGGA AAACCTACGC 
AGAACAGTGC AGCCGAATAA GGACAACTGC CGTCGAGCTG AAAATGTGCT CCGTCTCTGG 
ATCATTGAGG CAAAGGACCT GGCCCCCAAG AAGAAATACT TCTGTGAGCT GTGCCTTGAT 
GACACTCTCT TTGCCCGCAC CACCAGCAAA ACGAAAGCAG ATAACATTTT CTGGGGGGAG 
CACTTTGAGT TCTACGGCCT CCCAGCTCTT CACAGCATCA CGGTTCACAT CTACAAGGAT 
GTGGAGAAGA AAAAGAAAAA GGACAAAAAC AATTACGTCG GCCTGGTCAA CATTCCCATG 
GCCACTGTAA CGGGGCGCCA GTTTGTGGAA AAATGGTACC CAGTCAGCAC ACCTACACCC 
AACAAAGGGA AATCAGGAGG CCCTTCCATT CGCATCAAGT CGCGTTATCA GACCATCACC 
ATCTTACCCA TGGAGCAATA CAAGGAGTTT GCAGAGTTTG TTACCAGCAA CTACACGATG 
CTGTGCTCTG TGCTGGAGCC AGTCATCAGC GTAAGGAATA AGGAAGAGAT GGCTTGTGCA 
ATGGTGCACA TTCTTCAGAG CACGGGGAGA GCCAAGGACT TCCTAACGGA TCTGGTGATG 
TCTGAGGTGG ATCGCTGCGG GGAACACGAT GTCTTGATCT TCAGGGAGAA CACACTTGCT 
ACCAAAGCTA TTGAGGAATA CCTGAAGTTG GTAGGGCAGA AGTATCTCCA TGATGCGCTA 
GGGGAGTTTA TCAAGGCTTT GTATGAGTCA GATGAAAACT GTGAAGTGGA CCCCAGCAAA 
TGTTCATCCA GTGAATTAAC AGACCATCAG AGCAACCTGA AAATGTGTTG TGAGCTGGCC 
TTCTGCAAGA TTATCAATTC CTACTGCGTG TTCCCTCGTG AGCTGAAGGA GGTGTTTGCA 
TCTTGGAAGC AGCAGTGCCT GAGCAGGGGC AAGCAGGACA TCAGCGAGCG GCTGATCAGT 
GCCTCCCTGT TCCTCCGCTT CCTCTGCCCC GCCATCATGT CCCCCAGCCT CTTCAGCCTC 
ATGCAGGAGT ATCCTGACGA CCGGACATCG CGCACGCTCA CACTCATTGC TAAAGTCATT 
CAGAATCTCG CCAATTTTGC TAAGTTTGGT AACAAGGAAG AGTACATGGC TTTCATGAAC 
GACTTTCTGG AACATGAGTG GGGGGGAATG AAGCGATTTC TCCTGGAGAT CTCTAATCCA 
GATACCATCT CAAACATGCC TGGATTTGAG GGCTATATCG ACCTGGGCAG AGAGCTCTCA 
GTTTTGCATT CCCTCTTATG GGAGGTTGTG TCCCAACTTG ATAAGGGTGA AAATTCCTTC 
CTACAGGCGA CCGTGGCAAA ACTGGGACCT CTTCCTCGTA TTCTTGCCGA CATCACCAAA 
TCAATGACCA ACCCTACGCC AATACAGCAG CAGCTGAGGC GTTTCACTGA GCACAGCTCT 
AGTCCAAACG TCAGTGGCAG TCTCTCCTCA GGGCTTCAGA AGATATTCGA GGACCCCACT 
GATGGTGACT TGCATAAACT GAAGTCTCCA ACCCAGGAAA ACATAGATGG CTATTTCAGG 
GGCAAGACCT TGCTGTTGGT TCAGCAGGCG TCCACCCAGA GCATGACTTA CTCAGATAAG 
GACGAAAGAG AGAGCATCTT GCCCAACGGA CGTAGCATCT CCCTCATGGA CCTGCAGGAT 
CCCCATACCG CTCACAGCGA CCACGCTTCC ATGATGCACG ACGTGCCTTT GCGCTTGGCC 
GGCAGCCAGC TCTCCATCAC CCAGGTGGCC AACATCAAGC AGCTGTGGGA GACCCAGAGC 
ACGCCCCAAA GCGCTCCCCA GGTGAGGAGG CCGCTGCACC CGGCTTTGAA CCAGCAGGGC 
AGCCTCCAGC CTCTGTCATT CCAGAACCCG GTGTACCACC TCAACAACCC ACCTGCGCCG 
TTGCCCAAGG CCTCCGTGGA CTCCAGCCTG GAGAACCTGA GCACCGCCAG CTCCCAGAGC 
CGAAGCAACA GCGAAGACTT CAAGCTCAGC GGGCCCAGCA ACAGCAGCAT GGAGGACTTC 
ACCAAGCGCA GCACGCAGAG CGAGGACTTC TCTAGGCGGC ACACGGTCCC GGACAAGCAC 
GTCCCCATCG CGTTGCCCCG CCAGAACAGC AGCAGCCAGG CACAGATCCG CAAAATGGAC 
CAGGCCGGGT TAGGGGCCAG GGCCAAAGCG CCGCAGTCGC TGCCGCACAG CGCGTCCCTG 
CGCAGCACGG GCAGCATGTC GGCCGTGTCG GGGGCCGTGG TGACTGAGCC CGTCCAGAAC 
GGCAGCCGCT CCCGGCAGCA GTCGTCCTCC TCCAGGGAGA GCCCTGTGCC GAAGGTGAGG 
GCCATTCAGA GGCAGCAAAC GCAGCAGGTC CAGTCTCCTG TGGACTCAGC CACAATGTCA 
CCAGTGGAAA GGACAGCAGC GTGGGTTCTC AATAACGGGC AGTACGAGGA GGTCGAGGAG 
GACACAGAGC AGAACCCAGA TGAAGCCAAG CATGCTGAGA AGTACGAACA AGAGATAGCA 
AAGCTGAAGG AACGCTTGAA AGTGTCGAGC CGCCGGCTGG AGGAGTACGA GCGGCGGCTC 
CTGGTGCAGG AGCAGCAAAT GCAGAAGCTG CTGATGGAGT ACAAGTCCAG GCTGGAGGAC 
AGCGAGGAGA GACTGCGCAG GCAACAGGAG GAGAAGGACA GCCAGATGAA AAGCATTATC 
AGCAGGCTCA TGGCAGTTGA AGAGGAACTG AAGAAAGATC ATGCAGAGAT GCAAGCTGTC 
ATCGATGCAA AGCAGAAAAT TATTGATGCA CAGGTCATTA ATGGGAATGA GATAATTCAA 
ACAGGAGAAG CGGATCGTGT CCCTGGACTC TGCCAACACG CGGCTGATGA GTGCCCTGAC 
GCAGGTGAAG GAGCGCTACA GCATGCAAGT CCGCAATGGC ATCTCCCCCA CCAACCCCAC 
CAAGCTTTCC ATCACGGAGA ATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015146006.1
CDS	88..3414
Translation	MGPPSWGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNADDRSRGLPKLKESRSHESLLSPGSAVEALDLGAEEKVFVKPLHSSILGQDFCFEVTYSSGSKCFSCSSAAERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYGLPALHSITVHIYKDVEKKKKKDKNNYVGLVNIPMATVTGRQFVEKWYPVSTPTPNKGKSGGPSIRIKSRYQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEEMACAMVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTLATKAIEEYLKLVGQKYLHDALGEFIKALYESDENCEVDPSKCSSSELTDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCLSRGKQDISERLISASLFLRFLCPAIMSPSLFSLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNMPGFEGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVAKLGPLPRILADITKSMTNPTPIQQQLRRFTEHSSSPNVSGSLSSGLQKIFEDPTDGDLHKLKSPTQENIDGYFRGKTLLLVQQASTQSMTYSDKDERESILPNGRSISLMDLQDPHTAHSDHASMMHDVPLRLAGSQLSITQVANIKQLWETQSTPQSAPQVRRPLHPALNQQGSLQPLSFQNPVYHLNNPPAPLPKASVDSSLENLSTASSQSRSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTVPDKHVPIALPRQNSSSQAQIRKMDQAGLGARAKAPQSLPHSASLRSTGSMSAVSGAVVTEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEVEEDTEQNPDEAKHAEKYEQEIAKLKERLKVSSRRLEEYERRLLVQEQQMQKLLMEYKSRLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQVINGNEIIQTGEADRVPGLCQHAADECPDAGEGALQHASPQWHLPHQPHQAFHHGEW

Target ORF information:

RefSeq Version	XM_015290520.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015290520.1

ORF Insert Sequence:

ATGGGGCCGC CAAGCTGGGG CTTCTTCAGT AAACGTCTGA AAGGGTCCAT CAAGAGGACA 
AAGAGTCAGT CAAAGCTGGA CAGGAACACA AGTTTCCGCC TCCCATCCCT CCGCAATGCT 
GATGACAGGT CACGTGGGCT GCCGAAGCTG AAGGAGTCCC GCTCTCATGA GTCCCTGCTG 
AGCCCCGGCA GCGCAGTGGA AGCCCTGGAC CTTGGGGCAG AAGAAAAGGT CTTTGTAAAG 
CCACTGCACA GCAGCATCCT CGGACAGGAC TTCTGCTTTG AGGTAACCTA CTCCAGCGGC 
AGTAAATGCT TCAGCTGTTC CTCTGCTGCA GAGAGGGATA AGTGGATGGA AAACCTACGC 
AGAACAGTGC AGCCGAATAA GGACAACTGC CGTCGAGCTG AAAATGTGCT CCGTCTCTGG 
ATCATTGAGG CAAAGGACCT GGCCCCCAAG AAGAAATACT TCTGTGAGCT GTGCCTTGAT 
GACACTCTCT TTGCCCGCAC CACCAGCAAA ACGAAAGCAG ATAACATTTT CTGGGGGGAG 
CACTTTGAGT TCTACGGCCT CCCAGCTCTT CACAGCATCA CGGTTCACAT CTACAAGGAT 
GTGGAGAAGA AAAAGAAAAA GGACAAAAAC AATTACGTCG GCCTGGTCAA CATTCCCATG 
GCCACTGTAA CGGGGCGCCA GTTTGTGGAA AAATGGTACC CAGTCAGCAC ACCTACACCC 
AACAAAGGGA AATCAGGAGG CCCTTCCATT CGCATCAAGT CGCGTTATCA GACCATCACC 
ATCTTACCCA TGGAGCAATA CAAGGAGTTT GCAGAGTTTG TTACCAGCAA CTACACGATG 
CTGTGCTCTG TGCTGGAGCC AGTCATCAGC GTAAGGAATA AGGAAGAGAT GGCTTGTGCA 
ATGGTGCACA TTCTTCAGAG CACGGGGAGA GCCAAGGACT TCCTAACGGA TCTGGTGATG 
TCTGAGGTGG ATCGCTGCGG GGAACACGAT GTCTTGATCT TCAGGGAGAA CACACTTGCT 
ACCAAAGCTA TTGAGGAATA CCTGAAGTTG GTAGGGCAGA AGTATCTCCA TGATGCGCTA 
GGGGAGTTTA TCAAGGCTTT GTATGAGTCA GATGAAAACT GTGAAGTGGA CCCCAGCAAA 
TGTTCATCCA GTGAATTAAC AGACCATCAG AGCAACCTGA AAATGTGTTG TGAGCTGGCC 
TTCTGCAAGA TTATCAATTC CTACTGCGTG TTCCCTCGTG AGCTGAAGGA GGTGTTTGCA 
TCTTGGAAGC AGCAGTGCCT GAGCAGGGGC AAGCAGGACA TCAGCGAGCG GCTGATCAGT 
GCCTCCCTGT TCCTCCGCTT CCTCTGCCCC GCCATCATGT CCCCCAGCCT CTTCAGCCTC 
ATGCAGGAGT ATCCTGACGA CCGGACATCG CGCACGCTCA CACTCATTGC TAAAGTCATT 
CAGAATCTCG CCAATTTTGC TAAGTTTGGT AACAAGGAAG AGTACATGGC TTTCATGAAC 
GACTTTCTGG AACATGAGTG GGGGGGAATG AAGCGATTTC TCCTGGAGAT CTCTAATCCA 
GATACCATCT CAAACATGCC TGGATTTGAG GGCTATATCG ACCTGGGCAG AGAGCTCTCA 
GTTTTGCATT CCCTCTTATG GGAGGTTGTG TCCCAACTTG ATAAGGGTGA AAATTCCTTC 
CTACAGGCGA CCGTGGCAAA ACTGGGACCT CTTCCTCGTA TTCTTGCCGA CATCACCAAA 
TCAATGACCA ACCCTACGCC AATACAGCAG CAGCTGAGGC GTTTCACTGA GCACAGCTCT 
AGTCCAAACG TCAGTGGCAG TCTCTCCTCA GGGCTTCAGA AGATATTCGA GGACCCCACT 
GATGGTGACT TGCATAAACT GAAGTCTCCA ACCCAGGAAA ACATAGATGG CTATTTCAGG 
GGCAAGACCT TGCTGTTGGT TCAGCAGGCG TCCACCCAGA GCATGACTTA CTCAGATAAG 
GACGAAAGAG AGAGCATCTT GCCCAACGGA CGTAGCATCT CCCTCATGGA CCTGCAGGAT 
CCCCATACCG CTCACAGCGA CCACGCTTCC ATGATGCACG ACGTGCCTTT GCGCTTGGCC 
GGCAGCCAGC TCTCCATCAC CCAGGTGGCC AACATCAAGC AGCTGTGGGA GACCCAGAGC 
ACGCCCCAAA GCGCTCCCCA GGTGAGGAGG CCGCTGCACC CGGCTTTGAA CCAGCAGGGC 
AGCCTCCAGC CTCTGTCATT CCAGAACCCG GTGTACCACC TCAACAACCC ACCTGCGCCG 
TTGCCCAAGG CCTCCGTGGA CTCCAGCCTG GAGAACCTGA GCACCGCCAG CTCCCAGAGC 
CGAAGCAACA GCGAAGACTT CAAGCTCAGC GGGCCCAGCA ACAGCAGCAT GGAGGACTTC 
ACCAAGCGCA GCACGCAGAG CGAGGACTTC TCTAGGCGGC ACACGGTCCC GGACAAGCAC 
GTCCCCATCG CGTTGCCCCG CCAGAACAGC AGCAGCCAGG CACAGATCCG CAAAATGGAC 
CAGGCCGGGT TAGGGGCCAG GGCCAAAGCG CCGCAGTCGC TGCCGCACAG CGCGTCCCTG 
CGCAGCACGG GCAGCATGTC GGCCGTGTCG GGGGCCGTGG TGACTGAGCC CGTCCAGAAC 
GGCAGCCGCT CCCGGCAGCA GTCGTCCTCC TCCAGGGAGA GCCCTGTGCC GAAGGTGAGG 
GCCATTCAGA GGCAGCAAAC GCAGCAGGTC CAGTCTCCTG TGGACTCAGC CACAATGTCA 
CCAGTGGAAA GGACAGCAGC GTGGGTTCTC AATAACGGGC AGTACGAGGA GGTCGAGGAG 
GACACAGAGC AGAACCCAGA TGAAGCCAAG CATGCTGAGA AGTACGAACA AGAGATAGCA 
AAGCTGAAGG AACGCTTGAA AGTGTCGAGC CGCCGGCTGG AGGAGTACGA GCGGCGGCTC 
CTGGTGCAGG AGCAGCAAAT GCAGAAGCTG CTGATGGAGT ACAAGTCCAG GCTGGAGGAC 
AGCGAGGAGA GACTGCGCAG GCAACAGGAG GAGAAGGACA GCCAGATGAA AAGCATTATC 
AGCAGGCTCA TGGCAGTTGA AGAGGAACTG AAGAAAGATC ATGCAGAGAT GCAAGCTGTC 
ATCGATGCAA AGCAGAAAAT TATTGATGCA CAGGTCATTA ATGGGAATGA GATAATTCAA 
ACAGGAGAAG CGGATCGTGT CCCTGGACTC TGCCAACACG CGGCTGATGA GTGCCCTGAC 
GCAGGTGAAG GAGCGCTACA GCATGCAAGT CCGCAATGGC ATCTCCCCCA CCAACCCCAC 
CAAGCTTTCC ATCACGGAGA ATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_004943250.3 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004943307.1 Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OGa11742
Clone ID Related Accession (Same CDS sequence)	XM_004943250.3
Accession Version	XM_004943250.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3819bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	ras GTPase-activating protein nGAP isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006095.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004943250.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCGG CCCCCGCGGC GAGCGAGCCG GCGGCCTGGG CGGCCGAGGC GCTGGCGGAG 
CCGGAGCTGG GCTCGGACGA GCTGGAGGCG GCGGGCGGAG CGCTGGACCG CGTCCTGCTG 
GACTCGGTGT GCCAGCAGCA GGGCTGGGTC CGCGTCTACG ATGTAAAAGG ACCACCTACG 
CACAGGCTGT CCTGCGGCCA GTCCCCCTAC ACCGAGACGA CAACATGGGA GAGGAAGTAC 
TGCATCCTGA CTGACAGCCA GCTGGTGCTG CTCAACAGGG AGAAGGAGGT GCCCACCGAG 
GGGTTGCAGG AGCCGCAGGA TGCAACCAAA GGCCGCTGTC TGCGCCGCAC GGTGAGCGTG 
CCCTCCGAGG GCCAGTTCCC CGAGTACCCA CCAGATGGGG CCGCCAAGCT GGAGGTCCCG 
GCAGAGAGAT CTCCTCGCAG ACGGAGCATC TCTGGGACCA GCACATCTGA GAAAACCAAC 
TCCATGGATG CTGCAAATAC CTCTCCTTTC AAAGTATCGG GCTTCTTCAG TAAACGTCTG 
AAAGGGTCCA TCAAGAGGAC AAAGAGTCAG TCAAAGCTGG ACAGGAACAC AAGTTTCCGC 
CTCCCATCCC TCCGCAATGC TGATGACAGG TCACGTGGGC TGCCGAAGCT GAAGGAGTCC 
CGCTCTCATG AGTCCCTGCT GAGCCCCGGC AGCGCAGTGG AAGCCCTGGA CCTTGGGGCA 
GAAGAAAAGG TCTTTGTAAA GCCACTGCAC AGCAGCATCC TCGGACAGGA CTTCTGCTTT 
GAGGTAACCT ACTCCAGCGG CAGTAAATGC TTCAGCTGTT CCTCTGCTGC AGAGAGGGAT 
AAGTGGATGG AAAACCTACG CAGAACAGTG CAGCCGAATA AGGACAACTG CCGTCGAGCT 
GAAAATGTGC TCCGTCTCTG GATCATTGAG GCAAAGGACC TGGCCCCCAA GAAGAAATAC 
TTCTGTGAGC TGTGCCTTGA TGACACTCTC TTTGCCCGCA CCACCAGCAA AACGAAAGCA 
GATAACATTT TCTGGGGGGA GCACTTTGAG TTCTACGGCC TCCCAGCTCT TCACAGCATC 
ACGGTTCACA TCTACAAGGA TGTGGAGAAG AAAAAGAAAA AGGACAAAAA CAATTACGTC 
GGCCTGGTCA ACATTCCCAT GGCCACTGTA ACGGGGCGCC AGTTTGTGGA AAAATGGTAC 
CCAGTCAGCA CACCTACACC CAACAAAGGG AAATCAGGAG GCCCTTCCAT TCGCATCAAG 
TCGCGTTATC AGACCATCAC CATCTTACCC ATGGAGCAAT ACAAGGAGTT TGCAGAGTTT 
GTTACCAGCA ACTACACGAT GCTGTGCTCT GTGCTGGAGC CAGTCATCAG CGTAAGGAAT 
AAGGAAGAGA TGGCTTGTGC AATGGTGCAC ATTCTTCAGA GCACGGGGAG AGCCAAGGAC 
TTCCTAACGG ATCTGGTGAT GTCTGAGGTG GATCGCTGCG GGGAACACGA TGTCTTGATC 
TTCAGGGAGA ACACACTTGC TACCAAAGCT ATTGAGGAAT ACCTGAAGTT GGTAGGGCAG 
AAGTATCTCC ATGATGCGCT AGGGGAGTTT ATCAAGGCTT TGTATGAGTC AGATGAAAAC 
TGTGAAGTGG ACCCCAGCAA ATGTTCATCC AGTGAATTAA CAGACCATCA GAGCAACCTG 
AAAATGTGTT GTGAGCTGGC CTTCTGCAAG ATTATCAATT CCTACTGCGT GTTCCCTCGT 
GAGCTGAAGG AGGTGTTTGC ATCTTGGAAG CAGCAGTGCC TGAGCAGGGG CAAGCAGGAC 
ATCAGCGAGC GGCTGATCAG TGCCTCCCTG TTCCTCCGCT TCCTGTGCCC CGCCATCATG 
TCCCCCAGCC TCTTCAGCCT CATGCAGGAG TATCCTGACG ACCGGACATC GCGCACGCTC 
ACACTCATTG CTAAAGTCAT TCAGAATCTC GCCAATTTTG CTAAGTTTGG TAACAAGGAA 
GAGTACATGG CTTTCATGAA CGACTTTCTG GAACATGAGT GGGGGGGAAT GAAGCGATTT 
CTCCTGGAGA TCTCTAATCC AGATACCATC TCAAACATGC CTGGATTTGA GGGCTATATC 
GACCTGGGCA GAGAGCTCTC AGTTTTGCAT TCCCTCTTAT GGGAGGTTGT GTCCCAACTT 
GATAAGGGTG AAAATTCCTT CCTACAGGCG ACCGTGGCAA AACTGGGACC TCTTCCTCGT 
ATTCTTGCCG ACATCACCAA ATCAATGACC AACCCTACGC CAATACAGCA GCAGCTGAGG 
CGTTTCACTG AGCACAGCTC TAGTCCAAAC GTCAGTGGCA GTCTCTCCTC AGGGCTTCAG 
AAGATATTCG AGGACCCCAC TGATGGTGAC TTGCATAAAC TGAAGTCTCC AACCCAGGAA 
AACATAGATG GCTATTTCAG GGGCAAGACC TTGCTGTTGG TTCAGCAGGC GTCCACCCAG 
AGCATGACTT ACTCAGATAA GGACGAAAGA GAGAGCATCT TGCCCAACGG ACGTAGCATC 
TCCCTCATGG ACCTGCAGGA TCCCCATACC GCTCACAGCG ACCACGCTTC CATGATGCAC 
GACGTGCCTT TGCGCTTGGC CGGCAGCCAG CTCTCCATCA CCCAGGTGGC CAACATCAAG 
CAGCTGTGGG AGACCCAGAG CACGCCCCAA AGCGCTCCCC AGGTGAGGAG GCCGCTGCAC 
CCGGCTTTGA ACCAGCAGGG CAGCCTCCAG CCTCTGTCAT TCCAGAACCC GGTGTACCAC 
CTCAACAACC CACCTGCGCC GTTGCCCAAG GCCTCCGTGG ACTCCAGCCT GGAGAACCTG 
AGCACCGCCA GCTCCCAGAG CCGAAGCAAC AGCGAAGACT TCAAGCTCAG CGGGCCCAGC 
AACAGCAGCA TGGAGGACTT CACCAAGCGC AGCACGCAGA GCGAGGACTT CTCTAGGCGG 
CACACGGTCC CGGACAAGCA CGTCCCCATC GCGTTGCCCC GCCAGAACAG CAGCAGCCAG 
GCACAGATCC GCAAAATGGA CCAGGCCGGG TTAGGGGCCA GGGCCAAAGC GCCGCAGTCG 
CTGCCGCACA GCGCGTCCCT GCGCAGCACG GGCAGCATGT CGGCCGTGTC GGGGGCCGTG 
GTGACTGAGC CCGTCCAGAA CGGCAGCCGC TCCCGGCAGC AGTCGTCCTC CTCCAGGGAG 
AGCCCTGTGC CGAAGGTGAG GGCCATTCAG AGGCAGCAAA CGCAGCAGGT CCAGTCTCCT 
GTGGACTCAG CCACAATGTC ACCAGTGGAA AGGACAGCAG CGTGGGTTCT CAATAACGGG 
CAGTACGAGG AGGTCGAGGA GGACACAGAG CAGAACCCAG ATGAAGCCAA GCATGCTGAG 
AAGTACGAAC AAGAGATAGC AAAGCTGAAG GAACGCTTGA AAGTGTCGAG CCGCCGGCTG 
GAGGAGTACG AGCGGCGGCT CCTGGTGCAG GAGCAGCAAA TGCAGAAGCT GCTGATGGAG 
TACAAGTCCA GGCTGGAGGA CAGCGAGGAG AGACTGCGCA GGCAACAGGA GGAGAAGGAC 
AGCCAGATGA AAAGCATTAT CAGCAGGCTC ATGGCAGTTG AAGAGGAACT GAAGAAAGAT 
CATGCAGAGA TGCAAGCTGT CATCGATGCA AAGCAGAAAA TTATTGATGC ACAGGAGAAG 
CGGATCGTGT CCCTGGACTC TGCCAACACG CGGCTGATGA GTGCCCTGAC GCAGGTGAAG 
GAGCGCTACA GCATGCAAGT CCGCAATGGC ATCTCCCCCA CCAACCCCAC CAAGCTTTCC 
ATCACGGAGA ATGGTGAATT CAAAAACAGC AGCTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004943307.1
CDS	74..3892
Translation	MEAAPAASEPAAWAAEALAEPELGSDELEAAGGALDRVLLDSVCQQQGWVRVYDVKGPPTHRLSCGQSPYTETTTWERKYCILTDSQLVLLNREKEVPTEGLQEPQDATKGRCLRRTVSVPSEGQFPEYPPDGAAKLEVPAERSPRRRSISGTSTSEKTNSMDAANTSPFKVSGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNADDRSRGLPKLKESRSHESLLSPGSAVEALDLGAEEKVFVKPLHSSILGQDFCFEVTYSSGSKCFSCSSAAERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYGLPALHSITVHIYKDVEKKKKKDKNNYVGLVNIPMATVTGRQFVEKWYPVSTPTPNKGKSGGPSIRIKSRYQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEEMACAMVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTLATKAIEEYLKLVGQKYLHDALGEFIKALYESDENCEVDPSKCSSSELTDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCLSRGKQDISERLISASLFLRFLCPAIMSPSLFSLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNMPGFEGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVAKLGPLPRILADITKSMTNPTPIQQQLRRFTEHSSSPNVSGSLSSGLQKIFEDPTDGDLHKLKSPTQENIDGYFRGKTLLLVQQASTQSMTYSDKDERESILPNGRSISLMDLQDPHTAHSDHASMMHDVPLRLAGSQLSITQVANIKQLWETQSTPQSAPQVRRPLHPALNQQGSLQPLSFQNPVYHLNNPPAPLPKASVDSSLENLSTASSQSRSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTVPDKHVPIALPRQNSSSQAQIRKMDQAGLGARAKAPQSLPHSASLRSTGSMSAVSGAVVTEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEVEEDTEQNPDEAKHAEKYEQEIAKLKERLKVSSRRLEEYERRLLVQEQQMQKLLMEYKSRLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQEKRIVSLDSANTRLMSALTQVKERYSMQVRNGISPTNPTKLSITENGEFKNSSC

Target ORF information:

RefSeq Version	XM_004943250.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004943250.3

ORF Insert Sequence:

ATGGAGGCGG CCCCCGCGGC GAGCGAGCCG GCGGCCTGGG CGGCCGAGGC GCTGGCGGAG 
CCGGAGCTGG GCTCGGACGA GCTGGAGGCG GCGGGCGGAG CGCTGGACCG CGTCCTGCTG 
GACTCGGTGT GCCAGCAGCA GGGCTGGGTC CGCGTCTACG ATGTAAAAGG ACCACCTACG 
CACAGGCTGT CCTGCGGCCA GTCCCCCTAC ACCGAGACGA CAACATGGGA GAGGAAGTAC 
TGCATCCTGA CTGACAGCCA GCTGGTGCTG CTCAACAGGG AGAAGGAGGT GCCCACCGAG 
GGGTTGCAGG AGCCGCAGGA TGCAACCAAA GGCCGCTGTC TGCGCCGCAC GGTGAGCGTG 
CCCTCCGAGG GCCAGTTCCC CGAGTACCCA CCAGATGGGG CCGCCAAGCT GGAGGTCCCG 
GCAGAGAGAT CTCCTCGCAG ACGGAGCATC TCTGGGACCA GCACATCTGA GAAAACCAAC 
TCCATGGATG CTGCAAATAC CTCTCCTTTC AAAGTATCGG GCTTCTTCAG TAAACGTCTG 
AAAGGGTCCA TCAAGAGGAC AAAGAGTCAG TCAAAGCTGG ACAGGAACAC AAGTTTCCGC 
CTCCCATCCC TCCGCAATGC TGATGACAGG TCACGTGGGC TGCCGAAGCT GAAGGAGTCC 
CGCTCTCATG AGTCCCTGCT GAGCCCCGGC AGCGCAGTGG AAGCCCTGGA CCTTGGGGCA 
GAAGAAAAGG TCTTTGTAAA GCCACTGCAC AGCAGCATCC TCGGACAGGA CTTCTGCTTT 
GAGGTAACCT ACTCCAGCGG CAGTAAATGC TTCAGCTGTT CCTCTGCTGC AGAGAGGGAT 
AAGTGGATGG AAAACCTACG CAGAACAGTG CAGCCGAATA AGGACAACTG CCGTCGAGCT 
GAAAATGTGC TCCGTCTCTG GATCATTGAG GCAAAGGACC TGGCCCCCAA GAAGAAATAC 
TTCTGTGAGC TGTGCCTTGA TGACACTCTC TTTGCCCGCA CCACCAGCAA AACGAAAGCA 
GATAACATTT TCTGGGGGGA GCACTTTGAG TTCTACGGCC TCCCAGCTCT TCACAGCATC 
ACGGTTCACA TCTACAAGGA TGTGGAGAAG AAAAAGAAAA AGGACAAAAA CAATTACGTC 
GGCCTGGTCA ACATTCCCAT GGCCACTGTA ACGGGGCGCC AGTTTGTGGA AAAATGGTAC 
CCAGTCAGCA CACCTACACC CAACAAAGGG AAATCAGGAG GCCCTTCCAT TCGCATCAAG 
TCGCGTTATC AGACCATCAC CATCTTACCC ATGGAGCAAT ACAAGGAGTT TGCAGAGTTT 
GTTACCAGCA ACTACACGAT GCTGTGCTCT GTGCTGGAGC CAGTCATCAG CGTAAGGAAT 
AAGGAAGAGA TGGCTTGTGC AATGGTGCAC ATTCTTCAGA GCACGGGGAG AGCCAAGGAC 
TTCCTAACGG ATCTGGTGAT GTCTGAGGTG GATCGCTGCG GGGAACACGA TGTCTTGATC 
TTCAGGGAGA ACACACTTGC TACCAAAGCT ATTGAGGAAT ACCTGAAGTT GGTAGGGCAG 
AAGTATCTCC ATGATGCGCT AGGGGAGTTT ATCAAGGCTT TGTATGAGTC AGATGAAAAC 
TGTGAAGTGG ACCCCAGCAA ATGTTCATCC AGTGAATTAA CAGACCATCA GAGCAACCTG 
AAAATGTGTT GTGAGCTGGC CTTCTGCAAG ATTATCAATT CCTACTGCGT GTTCCCTCGT 
GAGCTGAAGG AGGTGTTTGC ATCTTGGAAG CAGCAGTGCC TGAGCAGGGG CAAGCAGGAC 
ATCAGCGAGC GGCTGATCAG TGCCTCCCTG TTCCTCCGCT TCCTGTGCCC CGCCATCATG 
TCCCCCAGCC TCTTCAGCCT CATGCAGGAG TATCCTGACG ACCGGACATC GCGCACGCTC 
ACACTCATTG CTAAAGTCAT TCAGAATCTC GCCAATTTTG CTAAGTTTGG TAACAAGGAA 
GAGTACATGG CTTTCATGAA CGACTTTCTG GAACATGAGT GGGGGGGAAT GAAGCGATTT 
CTCCTGGAGA TCTCTAATCC AGATACCATC TCAAACATGC CTGGATTTGA GGGCTATATC 
GACCTGGGCA GAGAGCTCTC AGTTTTGCAT TCCCTCTTAT GGGAGGTTGT GTCCCAACTT 
GATAAGGGTG AAAATTCCTT CCTACAGGCG ACCGTGGCAA AACTGGGACC TCTTCCTCGT 
ATTCTTGCCG ACATCACCAA ATCAATGACC AACCCTACGC CAATACAGCA GCAGCTGAGG 
CGTTTCACTG AGCACAGCTC TAGTCCAAAC GTCAGTGGCA GTCTCTCCTC AGGGCTTCAG 
AAGATATTCG AGGACCCCAC TGATGGTGAC TTGCATAAAC TGAAGTCTCC AACCCAGGAA 
AACATAGATG GCTATTTCAG GGGCAAGACC TTGCTGTTGG TTCAGCAGGC GTCCACCCAG 
AGCATGACTT ACTCAGATAA GGACGAAAGA GAGAGCATCT TGCCCAACGG ACGTAGCATC 
TCCCTCATGG ACCTGCAGGA TCCCCATACC GCTCACAGCG ACCACGCTTC CATGATGCAC 
GACGTGCCTT TGCGCTTGGC CGGCAGCCAG CTCTCCATCA CCCAGGTGGC CAACATCAAG 
CAGCTGTGGG AGACCCAGAG CACGCCCCAA AGCGCTCCCC AGGTGAGGAG GCCGCTGCAC 
CCGGCTTTGA ACCAGCAGGG CAGCCTCCAG CCTCTGTCAT TCCAGAACCC GGTGTACCAC 
CTCAACAACC CACCTGCGCC GTTGCCCAAG GCCTCCGTGG ACTCCAGCCT GGAGAACCTG 
AGCACCGCCA GCTCCCAGAG CCGAAGCAAC AGCGAAGACT TCAAGCTCAG CGGGCCCAGC 
AACAGCAGCA TGGAGGACTT CACCAAGCGC AGCACGCAGA GCGAGGACTT CTCTAGGCGG 
CACACGGTCC CGGACAAGCA CGTCCCCATC GCGTTGCCCC GCCAGAACAG CAGCAGCCAG 
GCACAGATCC GCAAAATGGA CCAGGCCGGG TTAGGGGCCA GGGCCAAAGC GCCGCAGTCG 
CTGCCGCACA GCGCGTCCCT GCGCAGCACG GGCAGCATGT CGGCCGTGTC GGGGGCCGTG 
GTGACTGAGC CCGTCCAGAA CGGCAGCCGC TCCCGGCAGC AGTCGTCCTC CTCCAGGGAG 
AGCCCTGTGC CGAAGGTGAG GGCCATTCAG AGGCAGCAAA CGCAGCAGGT CCAGTCTCCT 
GTGGACTCAG CCACAATGTC ACCAGTGGAA AGGACAGCAG CGTGGGTTCT CAATAACGGG 
CAGTACGAGG AGGTCGAGGA GGACACAGAG CAGAACCCAG ATGAAGCCAA GCATGCTGAG 
AAGTACGAAC AAGAGATAGC AAAGCTGAAG GAACGCTTGA AAGTGTCGAG CCGCCGGCTG 
GAGGAGTACG AGCGGCGGCT CCTGGTGCAG GAGCAGCAAA TGCAGAAGCT GCTGATGGAG 
TACAAGTCCA GGCTGGAGGA CAGCGAGGAG AGACTGCGCA GGCAACAGGA GGAGAAGGAC 
AGCCAGATGA AAAGCATTAT CAGCAGGCTC ATGGCAGTTG AAGAGGAACT GAAGAAAGAT 
CATGCAGAGA TGCAAGCTGT CATCGATGCA AAGCAGAAAA TTATTGATGC ACAGGAGAAG 
CGGATCGTGT CCCTGGACTC TGCCAACACG CGGCTGATGA GTGCCCTGAC GCAGGTGAAG 
GAGCGCTACA GCATGCAAGT CCGCAATGGC ATCTCCCCCA CCAACCCCAC CAAGCTTTCC 
ATCACGGAGA ATGGTGAATT CAAAAACAGC AGCTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_015290513.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015145999.1 Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OGa39044
Clone ID Related Accession (Same CDS sequence)	XM_015290513.1
Accession Version	XM_015290513.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3828bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	ras GTPase-activating protein nGAP isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCGG CCCCCGCGGC GAGCGAGCCG GCGGCCTGGG CGGCCGAGGC GCTGGCGGAG 
CCGGAGCTGG GCTCGGACGA GCTGGAGGCG GCGGGCGGAG CGCTGGACCG CGTCCTGCTG 
GACTCGGTGT GCCAGCAGCA GGGCTGGGTC CGCGTCTACG ATGTAAAAGG ACCACCTACG 
CACAGGCTGT CCTGCGGCCA GTCCCCCTAC ACCGAGACGA CAACATGGGA GAGGAAGTAC 
TGCATCCTGA CTGACAGCCA GCTGGTGCTG CTCAACAGGG AGAAGGAGGT GCCCACCGAG 
GGGTTGCAGG AGCCGCAGGA TGCAACCAAA GGCCGCTGTC TGCGCCGCAC GGTGAGCGTG 
CCCTCCGAGG GCCAGTTCCC CGAGTACCCA CCAGATGGGG CCGCCAAGCT GGAGGTCCCG 
GCAGAGAGAT CTCCTCGCAG ACGGAGCATC TCTGGGACCA GCACATCTGA GAAAACCAAC 
TCCATGGATG CTGCAAATAC CTCTCCTTTC AAAGTATCGG GCTTCTTCAG TAAACGTCTG 
AAAGGGTCCA TCAAGAGGAC AAAGAGTCAG TCAAAGCTGG ACAGGAACAC AAGTTTCCGC 
CTCCCATCCC TCCGCAATGC TGATGACAGG TCACGTGGGC TGCCGAAGCT GAAGGAGTCC 
CGCTCTCATG AGTCCCTGCT GAGCCCCGGC AGCGCAGTGG AAGCCCTGGA CCTTGGGGCA 
GAAGAAAAGG TCTTTGTAAA GCCACTGCAC AGCAGCATCC TCGGACAGGA CTTCTGCTTT 
GAGGTAACCT ACTCCAGCGG CAGTAAATGC TTCAGCTGTT CCTCTGCTGC AGAGAGGGAT 
AAGTGGATGG AAAACCTACG CAGAACAGTG CAGCCGAATA AGGACAACTG CCGTCGAGCT 
GAAAATGTGC TCCGTCTCTG GATCATTGAG GCAAAGGACC TGGCCCCCAA GAAGAAATAC 
TTCTGTGAGC TGTGCCTTGA TGACACTCTC TTTGCCCGCA CCACCAGCAA AACGAAAGCA 
GATAACATTT TCTGGGGGGA GCACTTTGAG TTCTACGGCC TCCCAGCTCT TCACAGCATC 
ACGGTTCACA TCTACAAGGA TGTGGAGAAG AAAAAGAAAA AGGACAAAAA CAATTACGTC 
GGCCTGGTCA ACATTCCCAT GGCCACTGTA ACGGGGCGCC AGTTTGTGGA AAAATGGTAC 
CCAGTCAGCA CACCTACACC CAACAAAGGG AAATCAGGAG GCCCTTCCAT TCGCATCAAG 
TCGCGTTATC AGACCATCAC CATCTTACCC ATGGAGCAAT ACAAGGAGTT TGCAGAGTTT 
GTTACCAGCA ACTACACGAT GCTGTGCTCT GTGCTGGAGC CAGTCATCAG CGTAAGGAAT 
AAGGAAGAGA TGGCTTGTGC AATGGTGCAC ATTCTTCAGA GCACGGGGAG AGCCAAGGAC 
TTCCTAACGG ATCTGGTGAT GTCTGAGGTG GATCGCTGCG GGGAACACGA TGTCTTGATC 
TTCAGGGAGA ACACACTTGC TACCAAAGCT ATTGAGGAAT ACCTGAAGTT GGTAGGGCAG 
AAGTATCTCC ATGATGCGCT AGGGGAGTTT ATCAAGGCTT TGTATGAGTC AGATGAAAAC 
TGTGAAGTGG ACCCCAGCAA ATGTTCATCC AGTGAATTAA CAGACCATCA GAGCAACCTG 
AAAATGTGTT GTGAGCTGGC CTTCTGCAAG ATTATCAATT CCTACTGCGT GTTCCCTCGT 
GAGCTGAAGG AGGTGTTTGC ATCTTGGAAG CAGCAGTGCC TGAGCAGGGG CAAGCAGGAC 
ATCAGCGAGC GGCTGATCAG TGCCTCCCTG TTCCTCCGCT TCCTCTGCCC CGCCATCATG 
TCCCCCAGCC TCTTCAGCCT CATGCAGGAG TATCCTGACG ACCGGACATC GCGCACGCTC 
ACACTCATTG CTAAAGTCAT TCAGAATCTC GCCAATTTTG CTAAGTTTGG TAACAAGGAA 
GAGTACATGG CTTTCATGAA CGACTTTCTG GAACATGAGT GGGGGGGAAT GAAGCGATTT 
CTCCTGGAGA TCTCTAATCC AGATACCATC TCAAACATGC CTGGATTTGA GGGCTATATC 
GACCTGGGCA GAGAGCTCTC AGTTTTGCAT TCCCTCTTAT GGGAGGTTGT GTCCCAACTT 
GATAAGGGTG AAAATTCCTT CCTACAGGCG ACCGTGGCAA AACTGGGACC TCTTCCTCGT 
ATTCTTGCCG ACATCACCAA ATCAATGACC AACCCTACGC CAATACAGCA GCAGCTGAGG 
CGTTTCACTG AGCACAGCTC TAGTCCAAAC GTCAGTGGCA GTCTCTCCTC AGGGCTTCAG 
AAGATATTCG AGGACCCCAC TGATGGTGAC TTGCATAAAC TGAAGTCTCC AACCCAGGAA 
AACATAGATG GCTATTTCAG GGGCAAGACC TTGCTGTTGG TTCAGCAGGC GTCCACCCAG 
AGCATGACTT ACTCAGATAA GGACGAAAGA GAGAGCATCT TGCCCAACGG ACGTAGCATC 
TCCCTCATGG ACCTGCAGGA TCCCCATACC GCTCACAGCG ACCACGCTTC CATGATGCAC 
GACGTGCCTT TGCGCTTGGC CGGCAGCCAG CTCTCCATCA CCCAGGTGGC CAACATCAAG 
CAGCTGTGGG AGACCCAGAG CACGCCCCAA AGCGCTCCCC AGGTGAGGAG GCCGCTGCAC 
CCGGCTTTGA ACCAGCAGGG CAGCCTCCAG CCTCTGTCAT TCCAGAACCC GGTGTACCAC 
CTCAACAACC CACCTGCGCC GTTGCCCAAG GCCTCCGTGG ACTCCAGCCT GGAGAACCTG 
AGCACCGCCA GCTCCCAGAG CCGAAGCAAC AGCGAAGACT TCAAGCTCAG CGGGCCCAGC 
AACAGCAGCA TGGAGGACTT CACCAAGCGC AGCACGCAGA GCGAGGACTT CTCTAGGCGG 
CACACGGTCC CGGACAAGCA CGTCCCCATC GCGTTGCCCC GCCAGAACAG CAGCAGCCAG 
GCACAGATCC GCAAAATGGA CCAGGCCGGG TTAGGGGCCA GGGCCAAAGC GCCGCAGTCG 
CTGCCGCACA GCGCGTCCCT GCGCAGCACG GGCAGCATGT CGGCCGTGTC GGGGGCCGTG 
GTGACTGAGC CCGTCCAGAA CGGCAGCCGC TCCCGGCAGC AGTCGTCCTC CTCCAGGGAG 
AGCCCTGTGC CGAAGGTGAG GGCCATTCAG AGGCAGCAAA CGCAGCAGGT CCAGTCTCCT 
GTGGACTCAG CCACAATGTC ACCAGTGGAA AGGACAGCAG CGTGGGTTCT CAATAACGGG 
CAGTACGAGG AGGTCGAGGA GGACACAGAG CAGAACCCAG ATGAAGCCAA GCATGCTGAG 
AAGTACGAAC AAGAGATAGC AAAGCTGAAG GAACGCTTGA AAGTGTCGAG CCGCCGGCTG 
GAGGAGTACG AGCGGCGGCT CCTGGTGCAG GAGCAGCAAA TGCAGAAGCT GCTGATGGAG 
TACAAGTCCA GGCTGGAGGA CAGCGAGGAG AGACTGCGCA GGCAACAGGA GGAGAAGGAC 
AGCCAGATGA AAAGCATTAT CAGCAGGCTC ATGGCAGTTG AAGAGGAACT GAAGAAAGAT 
CATGCAGAGA TGCAAGCTGT CATCGATGCA AAGCAGAAAA TTATTGATGC ACAGGTCATT 
AATGGGAATG AGATAATTCA AACAGGAGAA GCGGATCGTG TCCCTGGACT CTGCCAACAC 
GCGGCTGATG AGTGCCCTGA CGCAGGTGAA GGAGCGCTAC AGCATGCAAG TCCGCAATGG 
CATCTCCCCC ACCAACCCCA CCAAGCTTTC CATCACGGAG AATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015145999.1
CDS	78..3905
Translation	MEAAPAASEPAAWAAEALAEPELGSDELEAAGGALDRVLLDSVCQQQGWVRVYDVKGPPTHRLSCGQSPYTETTTWERKYCILTDSQLVLLNREKEVPTEGLQEPQDATKGRCLRRTVSVPSEGQFPEYPPDGAAKLEVPAERSPRRRSISGTSTSEKTNSMDAANTSPFKVSGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNADDRSRGLPKLKESRSHESLLSPGSAVEALDLGAEEKVFVKPLHSSILGQDFCFEVTYSSGSKCFSCSSAAERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYGLPALHSITVHIYKDVEKKKKKDKNNYVGLVNIPMATVTGRQFVEKWYPVSTPTPNKGKSGGPSIRIKSRYQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEEMACAMVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTLATKAIEEYLKLVGQKYLHDALGEFIKALYESDENCEVDPSKCSSSELTDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCLSRGKQDISERLISASLFLRFLCPAIMSPSLFSLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNMPGFEGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVAKLGPLPRILADITKSMTNPTPIQQQLRRFTEHSSSPNVSGSLSSGLQKIFEDPTDGDLHKLKSPTQENIDGYFRGKTLLLVQQASTQSMTYSDKDERESILPNGRSISLMDLQDPHTAHSDHASMMHDVPLRLAGSQLSITQVANIKQLWETQSTPQSAPQVRRPLHPALNQQGSLQPLSFQNPVYHLNNPPAPLPKASVDSSLENLSTASSQSRSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTVPDKHVPIALPRQNSSSQAQIRKMDQAGLGARAKAPQSLPHSASLRSTGSMSAVSGAVVTEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEVEEDTEQNPDEAKHAEKYEQEIAKLKERLKVSSRRLEEYERRLLVQEQQMQKLLMEYKSRLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQVINGNEIIQTGEADRVPGLCQHAADECPDAGEGALQHASPQWHLPHQPHQAFHHGEW

Target ORF information:

RefSeq Version	XM_015290513.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015290513.1

ORF Insert Sequence:

ATGGAGGCGG CCCCCGCGGC GAGCGAGCCG GCGGCCTGGG CGGCCGAGGC GCTGGCGGAG 
CCGGAGCTGG GCTCGGACGA GCTGGAGGCG GCGGGCGGAG CGCTGGACCG CGTCCTGCTG 
GACTCGGTGT GCCAGCAGCA GGGCTGGGTC CGCGTCTACG ATGTAAAAGG ACCACCTACG 
CACAGGCTGT CCTGCGGCCA GTCCCCCTAC ACCGAGACGA CAACATGGGA GAGGAAGTAC 
TGCATCCTGA CTGACAGCCA GCTGGTGCTG CTCAACAGGG AGAAGGAGGT GCCCACCGAG 
GGGTTGCAGG AGCCGCAGGA TGCAACCAAA GGCCGCTGTC TGCGCCGCAC GGTGAGCGTG 
CCCTCCGAGG GCCAGTTCCC CGAGTACCCA CCAGATGGGG CCGCCAAGCT GGAGGTCCCG 
GCAGAGAGAT CTCCTCGCAG ACGGAGCATC TCTGGGACCA GCACATCTGA GAAAACCAAC 
TCCATGGATG CTGCAAATAC CTCTCCTTTC AAAGTATCGG GCTTCTTCAG TAAACGTCTG 
AAAGGGTCCA TCAAGAGGAC AAAGAGTCAG TCAAAGCTGG ACAGGAACAC AAGTTTCCGC 
CTCCCATCCC TCCGCAATGC TGATGACAGG TCACGTGGGC TGCCGAAGCT GAAGGAGTCC 
CGCTCTCATG AGTCCCTGCT GAGCCCCGGC AGCGCAGTGG AAGCCCTGGA CCTTGGGGCA 
GAAGAAAAGG TCTTTGTAAA GCCACTGCAC AGCAGCATCC TCGGACAGGA CTTCTGCTTT 
GAGGTAACCT ACTCCAGCGG CAGTAAATGC TTCAGCTGTT CCTCTGCTGC AGAGAGGGAT 
AAGTGGATGG AAAACCTACG CAGAACAGTG CAGCCGAATA AGGACAACTG CCGTCGAGCT 
GAAAATGTGC TCCGTCTCTG GATCATTGAG GCAAAGGACC TGGCCCCCAA GAAGAAATAC 
TTCTGTGAGC TGTGCCTTGA TGACACTCTC TTTGCCCGCA CCACCAGCAA AACGAAAGCA 
GATAACATTT TCTGGGGGGA GCACTTTGAG TTCTACGGCC TCCCAGCTCT TCACAGCATC 
ACGGTTCACA TCTACAAGGA TGTGGAGAAG AAAAAGAAAA AGGACAAAAA CAATTACGTC 
GGCCTGGTCA ACATTCCCAT GGCCACTGTA ACGGGGCGCC AGTTTGTGGA AAAATGGTAC 
CCAGTCAGCA CACCTACACC CAACAAAGGG AAATCAGGAG GCCCTTCCAT TCGCATCAAG 
TCGCGTTATC AGACCATCAC CATCTTACCC ATGGAGCAAT ACAAGGAGTT TGCAGAGTTT 
GTTACCAGCA ACTACACGAT GCTGTGCTCT GTGCTGGAGC CAGTCATCAG CGTAAGGAAT 
AAGGAAGAGA TGGCTTGTGC AATGGTGCAC ATTCTTCAGA GCACGGGGAG AGCCAAGGAC 
TTCCTAACGG ATCTGGTGAT GTCTGAGGTG GATCGCTGCG GGGAACACGA TGTCTTGATC 
TTCAGGGAGA ACACACTTGC TACCAAAGCT ATTGAGGAAT ACCTGAAGTT GGTAGGGCAG 
AAGTATCTCC ATGATGCGCT AGGGGAGTTT ATCAAGGCTT TGTATGAGTC AGATGAAAAC 
TGTGAAGTGG ACCCCAGCAA ATGTTCATCC AGTGAATTAA CAGACCATCA GAGCAACCTG 
AAAATGTGTT GTGAGCTGGC CTTCTGCAAG ATTATCAATT CCTACTGCGT GTTCCCTCGT 
GAGCTGAAGG AGGTGTTTGC ATCTTGGAAG CAGCAGTGCC TGAGCAGGGG CAAGCAGGAC 
ATCAGCGAGC GGCTGATCAG TGCCTCCCTG TTCCTCCGCT TCCTCTGCCC CGCCATCATG 
TCCCCCAGCC TCTTCAGCCT CATGCAGGAG TATCCTGACG ACCGGACATC GCGCACGCTC 
ACACTCATTG CTAAAGTCAT TCAGAATCTC GCCAATTTTG CTAAGTTTGG TAACAAGGAA 
GAGTACATGG CTTTCATGAA CGACTTTCTG GAACATGAGT GGGGGGGAAT GAAGCGATTT 
CTCCTGGAGA TCTCTAATCC AGATACCATC TCAAACATGC CTGGATTTGA GGGCTATATC 
GACCTGGGCA GAGAGCTCTC AGTTTTGCAT TCCCTCTTAT GGGAGGTTGT GTCCCAACTT 
GATAAGGGTG AAAATTCCTT CCTACAGGCG ACCGTGGCAA AACTGGGACC TCTTCCTCGT 
ATTCTTGCCG ACATCACCAA ATCAATGACC AACCCTACGC CAATACAGCA GCAGCTGAGG 
CGTTTCACTG AGCACAGCTC TAGTCCAAAC GTCAGTGGCA GTCTCTCCTC AGGGCTTCAG 
AAGATATTCG AGGACCCCAC TGATGGTGAC TTGCATAAAC TGAAGTCTCC AACCCAGGAA 
AACATAGATG GCTATTTCAG GGGCAAGACC TTGCTGTTGG TTCAGCAGGC GTCCACCCAG 
AGCATGACTT ACTCAGATAA GGACGAAAGA GAGAGCATCT TGCCCAACGG ACGTAGCATC 
TCCCTCATGG ACCTGCAGGA TCCCCATACC GCTCACAGCG ACCACGCTTC CATGATGCAC 
GACGTGCCTT TGCGCTTGGC CGGCAGCCAG CTCTCCATCA CCCAGGTGGC CAACATCAAG 
CAGCTGTGGG AGACCCAGAG CACGCCCCAA AGCGCTCCCC AGGTGAGGAG GCCGCTGCAC 
CCGGCTTTGA ACCAGCAGGG CAGCCTCCAG CCTCTGTCAT TCCAGAACCC GGTGTACCAC 
CTCAACAACC CACCTGCGCC GTTGCCCAAG GCCTCCGTGG ACTCCAGCCT GGAGAACCTG 
AGCACCGCCA GCTCCCAGAG CCGAAGCAAC AGCGAAGACT TCAAGCTCAG CGGGCCCAGC 
AACAGCAGCA TGGAGGACTT CACCAAGCGC AGCACGCAGA GCGAGGACTT CTCTAGGCGG 
CACACGGTCC CGGACAAGCA CGTCCCCATC GCGTTGCCCC GCCAGAACAG CAGCAGCCAG 
GCACAGATCC GCAAAATGGA CCAGGCCGGG TTAGGGGCCA GGGCCAAAGC GCCGCAGTCG 
CTGCCGCACA GCGCGTCCCT GCGCAGCACG GGCAGCATGT CGGCCGTGTC GGGGGCCGTG 
GTGACTGAGC CCGTCCAGAA CGGCAGCCGC TCCCGGCAGC AGTCGTCCTC CTCCAGGGAG 
AGCCCTGTGC CGAAGGTGAG GGCCATTCAG AGGCAGCAAA CGCAGCAGGT CCAGTCTCCT 
GTGGACTCAG CCACAATGTC ACCAGTGGAA AGGACAGCAG CGTGGGTTCT CAATAACGGG 
CAGTACGAGG AGGTCGAGGA GGACACAGAG CAGAACCCAG ATGAAGCCAA GCATGCTGAG 
AAGTACGAAC AAGAGATAGC AAAGCTGAAG GAACGCTTGA AAGTGTCGAG CCGCCGGCTG 
GAGGAGTACG AGCGGCGGCT CCTGGTGCAG GAGCAGCAAA TGCAGAAGCT GCTGATGGAG 
TACAAGTCCA GGCTGGAGGA CAGCGAGGAG AGACTGCGCA GGCAACAGGA GGAGAAGGAC 
AGCCAGATGA AAAGCATTAT CAGCAGGCTC ATGGCAGTTG AAGAGGAACT GAAGAAAGAT 
CATGCAGAGA TGCAAGCTGT CATCGATGCA AAGCAGAAAA TTATTGATGC ACAGGTCATT 
AATGGGAATG AGATAATTCA AACAGGAGAA GCGGATCGTG TCCCTGGACT CTGCCAACAC 
GCGGCTGATG AGTGCCCTGA CGCAGGTGAA GGAGCGCTAC AGCATGCAAG TCCGCAATGG 
CATCTCCCCC ACCAACCCCA CCAAGCTTTC CATCACGGAG AATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_004943250.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004943307.1 Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X7, mRNA.

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CloneID	OGa39045
Clone ID Related Accession (Same CDS sequence)	XM_004943250.2
Accession Version	XM_004943250.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3819bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	ras GTPase-activating protein nGAP isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004943250.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCGG CCCCCGCGGC GAGCGAGCCG GCGGCCTGGG CGGCCGAGGC GCTGGCGGAG 
CCGGAGCTGG GCTCGGACGA GCTGGAGGCG GCGGGCGGAG CGCTGGACCG CGTCCTGCTG 
GACTCGGTGT GCCAGCAGCA GGGCTGGGTC CGCGTCTACG ATGTAAAAGG ACCACCTACG 
CACAGGCTGT CCTGCGGCCA GTCCCCCTAC ACCGAGACGA CAACATGGGA GAGGAAGTAC 
TGCATCCTGA CTGACAGCCA GCTGGTGCTG CTCAACAGGG AGAAGGAGGT GCCCACCGAG 
GGGTTGCAGG AGCCGCAGGA TGCAACCAAA GGCCGCTGTC TGCGCCGCAC GGTGAGCGTG 
CCCTCCGAGG GCCAGTTCCC CGAGTACCCA CCAGATGGGG CCGCCAAGCT GGAGGTCCCG 
GCAGAGAGAT CTCCTCGCAG ACGGAGCATC TCTGGGACCA GCACATCTGA GAAAACCAAC 
TCCATGGATG CTGCAAATAC CTCTCCTTTC AAAGTATCGG GCTTCTTCAG TAAACGTCTG 
AAAGGGTCCA TCAAGAGGAC AAAGAGTCAG TCAAAGCTGG ACAGGAACAC AAGTTTCCGC 
CTCCCATCCC TCCGCAATGC TGATGACAGG TCACGTGGGC TGCCGAAGCT GAAGGAGTCC 
CGCTCTCATG AGTCCCTGCT GAGCCCCGGC AGCGCAGTGG AAGCCCTGGA CCTTGGGGCA 
GAAGAAAAGG TCTTTGTAAA GCCACTGCAC AGCAGCATCC TCGGACAGGA CTTCTGCTTT 
GAGGTAACCT ACTCCAGCGG CAGTAAATGC TTCAGCTGTT CCTCTGCTGC AGAGAGGGAT 
AAGTGGATGG AAAACCTACG CAGAACAGTG CAGCCGAATA AGGACAACTG CCGTCGAGCT 
GAAAATGTGC TCCGTCTCTG GATCATTGAG GCAAAGGACC TGGCCCCCAA GAAGAAATAC 
TTCTGTGAGC TGTGCCTTGA TGACACTCTC TTTGCCCGCA CCACCAGCAA AACGAAAGCA 
GATAACATTT TCTGGGGGGA GCACTTTGAG TTCTACGGCC TCCCAGCTCT TCACAGCATC 
ACGGTTCACA TCTACAAGGA TGTGGAGAAG AAAAAGAAAA AGGACAAAAA CAATTACGTC 
GGCCTGGTCA ACATTCCCAT GGCCACTGTA ACGGGGCGCC AGTTTGTGGA AAAATGGTAC 
CCAGTCAGCA CACCTACACC CAACAAAGGG AAATCAGGAG GCCCTTCCAT TCGCATCAAG 
TCGCGTTATC AGACCATCAC CATCTTACCC ATGGAGCAAT ACAAGGAGTT TGCAGAGTTT 
GTTACCAGCA ACTACACGAT GCTGTGCTCT GTGCTGGAGC CAGTCATCAG CGTAAGGAAT 
AAGGAAGAGA TGGCTTGTGC AATGGTGCAC ATTCTTCAGA GCACGGGGAG AGCCAAGGAC 
TTCCTAACGG ATCTGGTGAT GTCTGAGGTG GATCGCTGCG GGGAACACGA TGTCTTGATC 
TTCAGGGAGA ACACACTTGC TACCAAAGCT ATTGAGGAAT ACCTGAAGTT GGTAGGGCAG 
AAGTATCTCC ATGATGCGCT AGGGGAGTTT ATCAAGGCTT TGTATGAGTC AGATGAAAAC 
TGTGAAGTGG ACCCCAGCAA ATGTTCATCC AGTGAATTAA CAGACCATCA GAGCAACCTG 
AAAATGTGTT GTGAGCTGGC CTTCTGCAAG ATTATCAATT CCTACTGCGT GTTCCCTCGT 
GAGCTGAAGG AGGTGTTTGC ATCTTGGAAG CAGCAGTGCC TGAGCAGGGG CAAGCAGGAC 
ATCAGCGAGC GGCTGATCAG TGCCTCCCTG TTCCTCCGCT TCCTCTGCCC CGCCATCATG 
TCCCCCAGCC TCTTCAGCCT CATGCAGGAG TATCCTGACG ACCGGACATC GCGCACGCTC 
ACACTCATTG CTAAAGTCAT TCAGAATCTC GCCAATTTTG CTAAGTTTGG TAACAAGGAA 
GAGTACATGG CTTTCATGAA CGACTTTCTG GAACATGAGT GGGGGGGAAT GAAGCGATTT 
CTCCTGGAGA TCTCTAATCC AGATACCATC TCAAACATGC CTGGATTTGA GGGCTATATC 
GACCTGGGCA GAGAGCTCTC AGTTTTGCAT TCCCTCTTAT GGGAGGTTGT GTCCCAACTT 
GATAAGGGTG AAAATTCCTT CCTACAGGCG ACCGTGGCAA AACTGGGACC TCTTCCTCGT 
ATTCTTGCCG ACATCACCAA ATCAATGACC AACCCTACGC CAATACAGCA GCAGCTGAGG 
CGTTTCACTG AGCACAGCTC TAGTCCAAAC GTCAGTGGCA GTCTCTCCTC AGGGCTTCAG 
AAGATATTCG AGGACCCCAC TGATGGTGAC TTGCATAAAC TGAAGTCTCC AACCCAGGAA 
AACATAGATG GCTATTTCAG GGGCAAGACC TTGCTGTTGG TTCAGCAGGC GTCCACCCAG 
AGCATGACTT ACTCAGATAA GGACGAAAGA GAGAGCATCT TGCCCAACGG ACGTAGCATC 
TCCCTCATGG ACCTGCAGGA TCCCCATACC GCTCACAGCG ACCACGCTTC CATGATGCAC 
GACGTGCCTT TGCGCTTGGC CGGCAGCCAG CTCTCCATCA CCCAGGTGGC CAACATCAAG 
CAGCTGTGGG AGACCCAGAG CACGCCCCAA AGCGCTCCCC AGGTGAGGAG GCCGCTGCAC 
CCGGCTTTGA ACCAGCAGGG CAGCCTCCAG CCTCTGTCAT TCCAGAACCC GGTGTACCAC 
CTCAACAACC CACCTGCGCC GTTGCCCAAG GCCTCCGTGG ACTCCAGCCT GGAGAACCTG 
AGCACCGCCA GCTCCCAGAG CCGAAGCAAC AGCGAAGACT TCAAGCTCAG CGGGCCCAGC 
AACAGCAGCA TGGAGGACTT CACCAAGCGC AGCACGCAGA GCGAGGACTT CTCTAGGCGG 
CACACGGTCC CGGACAAGCA CGTCCCCATC GCGTTGCCCC GCCAGAACAG CAGCAGCCAG 
GCACAGATCC GCAAAATGGA CCAGGCCGGG TTAGGGGCCA GGGCCAAAGC GCCGCAGTCG 
CTGCCGCACA GCGCGTCCCT GCGCAGCACG GGCAGCATGT CGGCCGTGTC GGGGGCCGTG 
GTGACTGAGC CCGTCCAGAA CGGCAGCCGC TCCCGGCAGC AGTCGTCCTC CTCCAGGGAG 
AGCCCTGTGC CGAAGGTGAG GGCCATTCAG AGGCAGCAAA CGCAGCAGGT CCAGTCTCCT 
GTGGACTCAG CCACAATGTC ACCAGTGGAA AGGACAGCAG CGTGGGTTCT CAATAACGGG 
CAGTACGAGG AGGTCGAGGA GGACACAGAG CAGAACCCAG ATGAAGCCAA GCATGCTGAG 
AAGTACGAAC AAGAGATAGC AAAGCTGAAG GAACGCTTGA AAGTGTCGAG CCGCCGGCTG 
GAGGAGTACG AGCGGCGGCT CCTGGTGCAG GAGCAGCAAA TGCAGAAGCT GCTGATGGAG 
TACAAGTCCA GGCTGGAGGA CAGCGAGGAG AGACTGCGCA GGCAACAGGA GGAGAAGGAC 
AGCCAGATGA AAAGCATTAT CAGCAGGCTC ATGGCAGTTG AAGAGGAACT GAAGAAAGAT 
CATGCAGAGA TGCAAGCTGT CATCGATGCA AAGCAGAAAA TTATTGATGC ACAGGAGAAG 
CGGATCGTGT CCCTGGACTC TGCCAACACG CGGCTGATGA GTGCCCTGAC GCAGGTGAAG 
GAGCGCTACA GCATGCAAGT CCGCAATGGC ATCTCCCCCA CCAACCCCAC CAAGCTTTCC 
ATCACGGAGA ATGGTGAATT CAAAAACAGC AGCTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004943307.1
CDS	77..3895
Translation	MEAAPAASEPAAWAAEALAEPELGSDELEAAGGALDRVLLDSVCQQQGWVRVYDVKGPPTHRLSCGQSPYTETTTWERKYCILTDSQLVLLNREKEVPTEGLQEPQDATKGRCLRRTVSVPSEGQFPEYPPDGAAKLEVPAERSPRRRSISGTSTSEKTNSMDAANTSPFKVSGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNADDRSRGLPKLKESRSHESLLSPGSAVEALDLGAEEKVFVKPLHSSILGQDFCFEVTYSSGSKCFSCSSAAERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYGLPALHSITVHIYKDVEKKKKKDKNNYVGLVNIPMATVTGRQFVEKWYPVSTPTPNKGKSGGPSIRIKSRYQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEEMACAMVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTLATKAIEEYLKLVGQKYLHDALGEFIKALYESDENCEVDPSKCSSSELTDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCLSRGKQDISERLISASLFLRFLCPAIMSPSLFSLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNMPGFEGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVAKLGPLPRILADITKSMTNPTPIQQQLRRFTEHSSSPNVSGSLSSGLQKIFEDPTDGDLHKLKSPTQENIDGYFRGKTLLLVQQASTQSMTYSDKDERESILPNGRSISLMDLQDPHTAHSDHASMMHDVPLRLAGSQLSITQVANIKQLWETQSTPQSAPQVRRPLHPALNQQGSLQPLSFQNPVYHLNNPPAPLPKASVDSSLENLSTASSQSRSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTVPDKHVPIALPRQNSSSQAQIRKMDQAGLGARAKAPQSLPHSASLRSTGSMSAVSGAVVTEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEVEEDTEQNPDEAKHAEKYEQEIAKLKERLKVSSRRLEEYERRLLVQEQQMQKLLMEYKSRLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQEKRIVSLDSANTRLMSALTQVKERYSMQVRNGISPTNPTKLSITENGEFKNSSC

Target ORF information:

RefSeq Version	XM_004943250.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004943250.2

ORF Insert Sequence:

ATGGAGGCGG CCCCCGCGGC GAGCGAGCCG GCGGCCTGGG CGGCCGAGGC GCTGGCGGAG 
CCGGAGCTGG GCTCGGACGA GCTGGAGGCG GCGGGCGGAG CGCTGGACCG CGTCCTGCTG 
GACTCGGTGT GCCAGCAGCA GGGCTGGGTC CGCGTCTACG ATGTAAAAGG ACCACCTACG 
CACAGGCTGT CCTGCGGCCA GTCCCCCTAC ACCGAGACGA CAACATGGGA GAGGAAGTAC 
TGCATCCTGA CTGACAGCCA GCTGGTGCTG CTCAACAGGG AGAAGGAGGT GCCCACCGAG 
GGGTTGCAGG AGCCGCAGGA TGCAACCAAA GGCCGCTGTC TGCGCCGCAC GGTGAGCGTG 
CCCTCCGAGG GCCAGTTCCC CGAGTACCCA CCAGATGGGG CCGCCAAGCT GGAGGTCCCG 
GCAGAGAGAT CTCCTCGCAG ACGGAGCATC TCTGGGACCA GCACATCTGA GAAAACCAAC 
TCCATGGATG CTGCAAATAC CTCTCCTTTC AAAGTATCGG GCTTCTTCAG TAAACGTCTG 
AAAGGGTCCA TCAAGAGGAC AAAGAGTCAG TCAAAGCTGG ACAGGAACAC AAGTTTCCGC 
CTCCCATCCC TCCGCAATGC TGATGACAGG TCACGTGGGC TGCCGAAGCT GAAGGAGTCC 
CGCTCTCATG AGTCCCTGCT GAGCCCCGGC AGCGCAGTGG AAGCCCTGGA CCTTGGGGCA 
GAAGAAAAGG TCTTTGTAAA GCCACTGCAC AGCAGCATCC TCGGACAGGA CTTCTGCTTT 
GAGGTAACCT ACTCCAGCGG CAGTAAATGC TTCAGCTGTT CCTCTGCTGC AGAGAGGGAT 
AAGTGGATGG AAAACCTACG CAGAACAGTG CAGCCGAATA AGGACAACTG CCGTCGAGCT 
GAAAATGTGC TCCGTCTCTG GATCATTGAG GCAAAGGACC TGGCCCCCAA GAAGAAATAC 
TTCTGTGAGC TGTGCCTTGA TGACACTCTC TTTGCCCGCA CCACCAGCAA AACGAAAGCA 
GATAACATTT TCTGGGGGGA GCACTTTGAG TTCTACGGCC TCCCAGCTCT TCACAGCATC 
ACGGTTCACA TCTACAAGGA TGTGGAGAAG AAAAAGAAAA AGGACAAAAA CAATTACGTC 
GGCCTGGTCA ACATTCCCAT GGCCACTGTA ACGGGGCGCC AGTTTGTGGA AAAATGGTAC 
CCAGTCAGCA CACCTACACC CAACAAAGGG AAATCAGGAG GCCCTTCCAT TCGCATCAAG 
TCGCGTTATC AGACCATCAC CATCTTACCC ATGGAGCAAT ACAAGGAGTT TGCAGAGTTT 
GTTACCAGCA ACTACACGAT GCTGTGCTCT GTGCTGGAGC CAGTCATCAG CGTAAGGAAT 
AAGGAAGAGA TGGCTTGTGC AATGGTGCAC ATTCTTCAGA GCACGGGGAG AGCCAAGGAC 
TTCCTAACGG ATCTGGTGAT GTCTGAGGTG GATCGCTGCG GGGAACACGA TGTCTTGATC 
TTCAGGGAGA ACACACTTGC TACCAAAGCT ATTGAGGAAT ACCTGAAGTT GGTAGGGCAG 
AAGTATCTCC ATGATGCGCT AGGGGAGTTT ATCAAGGCTT TGTATGAGTC AGATGAAAAC 
TGTGAAGTGG ACCCCAGCAA ATGTTCATCC AGTGAATTAA CAGACCATCA GAGCAACCTG 
AAAATGTGTT GTGAGCTGGC CTTCTGCAAG ATTATCAATT CCTACTGCGT GTTCCCTCGT 
GAGCTGAAGG AGGTGTTTGC ATCTTGGAAG CAGCAGTGCC TGAGCAGGGG CAAGCAGGAC 
ATCAGCGAGC GGCTGATCAG TGCCTCCCTG TTCCTCCGCT TCCTCTGCCC CGCCATCATG 
TCCCCCAGCC TCTTCAGCCT CATGCAGGAG TATCCTGACG ACCGGACATC GCGCACGCTC 
ACACTCATTG CTAAAGTCAT TCAGAATCTC GCCAATTTTG CTAAGTTTGG TAACAAGGAA 
GAGTACATGG CTTTCATGAA CGACTTTCTG GAACATGAGT GGGGGGGAAT GAAGCGATTT 
CTCCTGGAGA TCTCTAATCC AGATACCATC TCAAACATGC CTGGATTTGA GGGCTATATC 
GACCTGGGCA GAGAGCTCTC AGTTTTGCAT TCCCTCTTAT GGGAGGTTGT GTCCCAACTT 
GATAAGGGTG AAAATTCCTT CCTACAGGCG ACCGTGGCAA AACTGGGACC TCTTCCTCGT 
ATTCTTGCCG ACATCACCAA ATCAATGACC AACCCTACGC CAATACAGCA GCAGCTGAGG 
CGTTTCACTG AGCACAGCTC TAGTCCAAAC GTCAGTGGCA GTCTCTCCTC AGGGCTTCAG 
AAGATATTCG AGGACCCCAC TGATGGTGAC TTGCATAAAC TGAAGTCTCC AACCCAGGAA 
AACATAGATG GCTATTTCAG GGGCAAGACC TTGCTGTTGG TTCAGCAGGC GTCCACCCAG 
AGCATGACTT ACTCAGATAA GGACGAAAGA GAGAGCATCT TGCCCAACGG ACGTAGCATC 
TCCCTCATGG ACCTGCAGGA TCCCCATACC GCTCACAGCG ACCACGCTTC CATGATGCAC 
GACGTGCCTT TGCGCTTGGC CGGCAGCCAG CTCTCCATCA CCCAGGTGGC CAACATCAAG 
CAGCTGTGGG AGACCCAGAG CACGCCCCAA AGCGCTCCCC AGGTGAGGAG GCCGCTGCAC 
CCGGCTTTGA ACCAGCAGGG CAGCCTCCAG CCTCTGTCAT TCCAGAACCC GGTGTACCAC 
CTCAACAACC CACCTGCGCC GTTGCCCAAG GCCTCCGTGG ACTCCAGCCT GGAGAACCTG 
AGCACCGCCA GCTCCCAGAG CCGAAGCAAC AGCGAAGACT TCAAGCTCAG CGGGCCCAGC 
AACAGCAGCA TGGAGGACTT CACCAAGCGC AGCACGCAGA GCGAGGACTT CTCTAGGCGG 
CACACGGTCC CGGACAAGCA CGTCCCCATC GCGTTGCCCC GCCAGAACAG CAGCAGCCAG 
GCACAGATCC GCAAAATGGA CCAGGCCGGG TTAGGGGCCA GGGCCAAAGC GCCGCAGTCG 
CTGCCGCACA GCGCGTCCCT GCGCAGCACG GGCAGCATGT CGGCCGTGTC GGGGGCCGTG 
GTGACTGAGC CCGTCCAGAA CGGCAGCCGC TCCCGGCAGC AGTCGTCCTC CTCCAGGGAG 
AGCCCTGTGC CGAAGGTGAG GGCCATTCAG AGGCAGCAAA CGCAGCAGGT CCAGTCTCCT 
GTGGACTCAG CCACAATGTC ACCAGTGGAA AGGACAGCAG CGTGGGTTCT CAATAACGGG 
CAGTACGAGG AGGTCGAGGA GGACACAGAG CAGAACCCAG ATGAAGCCAA GCATGCTGAG 
AAGTACGAAC AAGAGATAGC AAAGCTGAAG GAACGCTTGA AAGTGTCGAG CCGCCGGCTG 
GAGGAGTACG AGCGGCGGCT CCTGGTGCAG GAGCAGCAAA TGCAGAAGCT GCTGATGGAG 
TACAAGTCCA GGCTGGAGGA CAGCGAGGAG AGACTGCGCA GGCAACAGGA GGAGAAGGAC 
AGCCAGATGA AAAGCATTAT CAGCAGGCTC ATGGCAGTTG AAGAGGAACT GAAGAAAGAT 
CATGCAGAGA TGCAAGCTGT CATCGATGCA AAGCAGAAAA TTATTGATGC ACAGGAGAAG 
CGGATCGTGT CCCTGGACTC TGCCAACACG CGGCTGATGA GTGCCCTGAC GCAGGTGAAG 
GAGCGCTACA GCATGCAAGT CCGCAATGGC ATCTCCCCCA CCAACCCCAC CAAGCTTTCC 
ATCACGGAGA ATGGTGAATT CAAAAACAGC AGCTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_015290514.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015146000.1 Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X8, mRNA.

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CloneID	OGa39046
Clone ID Related Accession (Same CDS sequence)	XM_015290514.1
Accession Version	XM_015290514.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3798bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	ras GTPase-activating protein nGAP isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCGG CCCCCGCGGC GAGCGAGCCG GCGGCCTGGG CGGCCGAGGC GCTGGCGGAG 
CCGGAGCTGG GCTCGGACGA GCTGGAGGCG GCGGGCGGAG CGCTGGACCG CGTCCTGCTG 
GACTCGGTGT GCCAGCAGCA GGGCTGGGTC CGCGTCTACG ATGTAAAAGG ACCACCTACG 
CACAGGCTGT CCTGCGGCCA GTCCCCCTAC ACCGAGACGA CAACATGGGA GAGGAAGTAC 
TGCATCCTGA CTGACAGCCA GCTGGTGCTG CTCAACAGGG AGAAGGAGGT GCCCACCGAG 
GGGTTGCAGG AGCCGCAGGA TGCAACCAAA GGCCGCTGTC TGCGCCGCAC GGTGAGCGTG 
CCCTCCGAGG GCCAGTTCCC CGAGTACCCA CCAGATGGGG CCGCCAAGCT GGAGGTCCCG 
GCAGAGAGAT CTCCTCGCAG ACGGAGCATC TCTGGGACCA GCACATCTGA GAAAACCAAC 
TCCATGGATG CTGCAAATAC CTCTCCTTTC AAAGTATCGG GCTTCTTCAG TAAACGTCTG 
AAAGGGTCCA TCAAGAGGAC AAAGAGTCAG TCAAAGCTGG ACAGGAACAC AAGTTTCCGC 
CTCCCATCCC TCCGCAATGC TGATGACAGG TCACGTGGGC TGCCGAAGCT GAAGGAGTCC 
CGCTCTCATG AGTCCCTGCT GAGCCCCGGC AGCGCAGTGG AAGCCCTGGA CCTTGGGGCA 
GAAGAAAAGG TCTTTGTAAA GCCACTGCAC AGCAGCATCC TCGGACAGGA CTTCTGCTTT 
GAGGTAACCT ACTCCAGCGG CAGTAAATGC TTCAGCTGTT CCTCTGCTGC AGAGAGGGAT 
AAGTGGATGG AAAACCTACG CAGAACAGTG CAGCCGAATA AGGACAACTG CCGTCGAGCT 
GAAAATGTGC TCCGTCTCTG GATCATTGAG GCAAAGGACC TGGCCCCCAA GAAGAAATAC 
TTCTGTGAGC TGTGCCTTGA TGACACTCTC TTTGCCCGCA CCACCAGCAA AACGAAAGCA 
GATAACATTT TCTGGGGGGA GCACTTTGAG TTCTACGGCC TCCCAGCTCT TCACAGCATC 
ACGGTTCACA TCTACAAGGA TGTGGAGAAG AAAAAGAAAA AGGACAAAAA CAATTACGTC 
GGCCTGGTCA ACATTCCCAT GGCCACTGTA ACGGGGCGCC AGTTTGTGGA AAAATGGTAC 
CCAGTCAGCA CACCTACACC CAACAAAGGG AAATCAGGAG GCCCTTCCAT TCGCATCAAG 
TCGCGTTATC AGACCATCAC CATCTTACCC ATGGAGCAAT ACAAGGAGTT TGCAGAGTTT 
GTTACCAGCA ACTACACGAT GCTGTGCTCT GTGCTGGAGC CAGTCATCAG CGTAAGGAAT 
AAGGAAGAGA TGGCTTGTGC AATGGTGCAC ATTCTTCAGA GCACGGGGAG AGCCAAGGAC 
TTCCTAACGG ATCTGGTGAT GTCTGAGGTG GATCGCTGCG GGGAACACGA TGTCTTGATC 
TTCAGGGAGA ACACACTTGC TACCAAAGCT ATTGAGGAAT ACCTGAAGTT GGTAGGGCAG 
AAGTATCTCC ATGATGCGCT AGGGGAGTTT ATCAAGGCTT TGTATGAGTC AGATGAAAAC 
TGTGAAGTGG ACCCCAGCAA ATGTTCATCC AGTGAATTAA CAGACCATCA GAGCAACCTG 
AAAATGTGTT GTGAGCTGGC CTTCTGCAAG ATTATCAATT CCTACTGCGT GTTCCCTCGT 
GAGCTGAAGG AGGTGTTTGC ATCTTGGAAG CAGCAGTGCC TGAGCAGGGG CAAGCAGGAC 
ATCAGCGAGC GGCTGATCAG TGCCTCCCTG TTCCTCCGCT TCCTCTGCCC CGCCATCATG 
TCCCCCAGCC TCTTCAGCCT CATGCAGGAG TATCCTGACG ACCGGACATC GCGCACGCTC 
ACACTCATTG CTAAAGTCAT TCAGAATCTC GCCAATTTTG CTAAGTTTGG TAACAAGGAA 
GAGTACATGG CTTTCATGAA CGACTTTCTG GAACATGAGT GGGGGGGAAT GAAGCGATTT 
CTCCTGGAGA TCTCTAATCC AGATACCATC TCAAACATGC CTGGATTTGA GGGCTATATC 
GACCTGGGCA GAGAGCTCTC AGTTTTGCAT TCCCTCTTAT GGGAGGTTGT GTCCCAACTT 
GATAAGGCGA CCGTGGCAAA ACTGGGACCT CTTCCTCGTA TTCTTGCCGA CATCACCAAA 
TCAATGACCA ACCCTACGCC AATACAGCAG CAGCTGAGGC GTTTCACTGA GCACAGCTCT 
AGTCCAAACG TCAGTGGCAG TCTCTCCTCA GGGCTTCAGA AGATATTCGA GGACCCCACT 
GATGGTGACT TGCATAAACT GAAGTCTCCA ACCCAGGAAA ACATAGATGG CTATTTCAGG 
GGCAAGACCT TGCTGTTGGT TCAGCAGGCG TCCACCCAGA GCATGACTTA CTCAGATAAG 
GACGAAAGAG AGAGCATCTT GCCCAACGGA CGTAGCATCT CCCTCATGGA CCTGCAGGAT 
CCCCATACCG CTCACAGCGA CCACGCTTCC ATGATGCACG ACGTGCCTTT GCGCTTGGCC 
GGCAGCCAGC TCTCCATCAC CCAGGTGGCC AACATCAAGC AGCTGTGGGA GACCCAGAGC 
ACGCCCCAAA GCGCTCCCCA GGTGAGGAGG CCGCTGCACC CGGCTTTGAA CCAGCAGGGC 
AGCCTCCAGC CTCTGTCATT CCAGAACCCG GTGTACCACC TCAACAACCC ACCTGCGCCG 
TTGCCCAAGG CCTCCGTGGA CTCCAGCCTG GAGAACCTGA GCACCGCCAG CTCCCAGAGC 
CGAAGCAACA GCGAAGACTT CAAGCTCAGC GGGCCCAGCA ACAGCAGCAT GGAGGACTTC 
ACCAAGCGCA GCACGCAGAG CGAGGACTTC TCTAGGCGGC ACACGGTCCC GGACAAGCAC 
GTCCCCATCG CGTTGCCCCG CCAGAACAGC AGCAGCCAGG CACAGATCCG CAAAATGGAC 
CAGGCCGGGT TAGGGGCCAG GGCCAAAGCG CCGCAGTCGC TGCCGCACAG CGCGTCCCTG 
CGCAGCACGG GCAGCATGTC GGCCGTGTCG GGGGCCGTGG TGACTGAGCC CGTCCAGAAC 
GGCAGCCGCT CCCGGCAGCA GTCGTCCTCC TCCAGGGAGA GCCCTGTGCC GAAGGTGAGG 
GCCATTCAGA GGCAGCAAAC GCAGCAGGTC CAGTCTCCTG TGGACTCAGC CACAATGTCA 
CCAGTGGAAA GGACAGCAGC GTGGGTTCTC AATAACGGGC AGTACGAGGA GGTCGAGGAG 
GACACAGAGC AGAACCCAGA TGAAGCCAAG CATGCTGAGA AGTACGAACA AGAGATAGCA 
AAGCTGAAGG AACGCTTGAA AGTGTCGAGC CGCCGGCTGG AGGAGTACGA GCGGCGGCTC 
CTGGTGCAGG AGCAGCAAAT GCAGAAGCTG CTGATGGAGT ACAAGTCCAG GCTGGAGGAC 
AGCGAGGAGA GACTGCGCAG GCAACAGGAG GAGAAGGACA GCCAGATGAA AAGCATTATC 
AGCAGGCTCA TGGCAGTTGA AGAGGAACTG AAGAAAGATC ATGCAGAGAT GCAAGCTGTC 
ATCGATGCAA AGCAGAAAAT TATTGATGCA CAGGAGAAGC GGATCGTGTC CCTGGACTCT 
GCCAACACGC GGCTGATGAG TGCCCTGACG CAGGTGAAGG AGCGCTACAG CATGCAAGTC 
CGCAATGGCA TCTCCCCCAC CAACCCCACC AAGCTTTCCA TCACGGAGAA TGGTGAATTC 
AAAAACAGCA GCTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015146000.1
CDS	77..3874
Translation	MEAAPAASEPAAWAAEALAEPELGSDELEAAGGALDRVLLDSVCQQQGWVRVYDVKGPPTHRLSCGQSPYTETTTWERKYCILTDSQLVLLNREKEVPTEGLQEPQDATKGRCLRRTVSVPSEGQFPEYPPDGAAKLEVPAERSPRRRSISGTSTSEKTNSMDAANTSPFKVSGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNADDRSRGLPKLKESRSHESLLSPGSAVEALDLGAEEKVFVKPLHSSILGQDFCFEVTYSSGSKCFSCSSAAERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYGLPALHSITVHIYKDVEKKKKKDKNNYVGLVNIPMATVTGRQFVEKWYPVSTPTPNKGKSGGPSIRIKSRYQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEEMACAMVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTLATKAIEEYLKLVGQKYLHDALGEFIKALYESDENCEVDPSKCSSSELTDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCLSRGKQDISERLISASLFLRFLCPAIMSPSLFSLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNMPGFEGYIDLGRELSVLHSLLWEVVSQLDKATVAKLGPLPRILADITKSMTNPTPIQQQLRRFTEHSSSPNVSGSLSSGLQKIFEDPTDGDLHKLKSPTQENIDGYFRGKTLLLVQQASTQSMTYSDKDERESILPNGRSISLMDLQDPHTAHSDHASMMHDVPLRLAGSQLSITQVANIKQLWETQSTPQSAPQVRRPLHPALNQQGSLQPLSFQNPVYHLNNPPAPLPKASVDSSLENLSTASSQSRSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTVPDKHVPIALPRQNSSSQAQIRKMDQAGLGARAKAPQSLPHSASLRSTGSMSAVSGAVVTEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEVEEDTEQNPDEAKHAEKYEQEIAKLKERLKVSSRRLEEYERRLLVQEQQMQKLLMEYKSRLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQEKRIVSLDSANTRLMSALTQVKERYSMQVRNGISPTNPTKLSITENGEFKNSSC

Target ORF information:

RefSeq Version	XM_015290514.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015290514.1

ORF Insert Sequence:

ATGGAGGCGG CCCCCGCGGC GAGCGAGCCG GCGGCCTGGG CGGCCGAGGC GCTGGCGGAG 
CCGGAGCTGG GCTCGGACGA GCTGGAGGCG GCGGGCGGAG CGCTGGACCG CGTCCTGCTG 
GACTCGGTGT GCCAGCAGCA GGGCTGGGTC CGCGTCTACG ATGTAAAAGG ACCACCTACG 
CACAGGCTGT CCTGCGGCCA GTCCCCCTAC ACCGAGACGA CAACATGGGA GAGGAAGTAC 
TGCATCCTGA CTGACAGCCA GCTGGTGCTG CTCAACAGGG AGAAGGAGGT GCCCACCGAG 
GGGTTGCAGG AGCCGCAGGA TGCAACCAAA GGCCGCTGTC TGCGCCGCAC GGTGAGCGTG 
CCCTCCGAGG GCCAGTTCCC CGAGTACCCA CCAGATGGGG CCGCCAAGCT GGAGGTCCCG 
GCAGAGAGAT CTCCTCGCAG ACGGAGCATC TCTGGGACCA GCACATCTGA GAAAACCAAC 
TCCATGGATG CTGCAAATAC CTCTCCTTTC AAAGTATCGG GCTTCTTCAG TAAACGTCTG 
AAAGGGTCCA TCAAGAGGAC AAAGAGTCAG TCAAAGCTGG ACAGGAACAC AAGTTTCCGC 
CTCCCATCCC TCCGCAATGC TGATGACAGG TCACGTGGGC TGCCGAAGCT GAAGGAGTCC 
CGCTCTCATG AGTCCCTGCT GAGCCCCGGC AGCGCAGTGG AAGCCCTGGA CCTTGGGGCA 
GAAGAAAAGG TCTTTGTAAA GCCACTGCAC AGCAGCATCC TCGGACAGGA CTTCTGCTTT 
GAGGTAACCT ACTCCAGCGG CAGTAAATGC TTCAGCTGTT CCTCTGCTGC AGAGAGGGAT 
AAGTGGATGG AAAACCTACG CAGAACAGTG CAGCCGAATA AGGACAACTG CCGTCGAGCT 
GAAAATGTGC TCCGTCTCTG GATCATTGAG GCAAAGGACC TGGCCCCCAA GAAGAAATAC 
TTCTGTGAGC TGTGCCTTGA TGACACTCTC TTTGCCCGCA CCACCAGCAA AACGAAAGCA 
GATAACATTT TCTGGGGGGA GCACTTTGAG TTCTACGGCC TCCCAGCTCT TCACAGCATC 
ACGGTTCACA TCTACAAGGA TGTGGAGAAG AAAAAGAAAA AGGACAAAAA CAATTACGTC 
GGCCTGGTCA ACATTCCCAT GGCCACTGTA ACGGGGCGCC AGTTTGTGGA AAAATGGTAC 
CCAGTCAGCA CACCTACACC CAACAAAGGG AAATCAGGAG GCCCTTCCAT TCGCATCAAG 
TCGCGTTATC AGACCATCAC CATCTTACCC ATGGAGCAAT ACAAGGAGTT TGCAGAGTTT 
GTTACCAGCA ACTACACGAT GCTGTGCTCT GTGCTGGAGC CAGTCATCAG CGTAAGGAAT 
AAGGAAGAGA TGGCTTGTGC AATGGTGCAC ATTCTTCAGA GCACGGGGAG AGCCAAGGAC 
TTCCTAACGG ATCTGGTGAT GTCTGAGGTG GATCGCTGCG GGGAACACGA TGTCTTGATC 
TTCAGGGAGA ACACACTTGC TACCAAAGCT ATTGAGGAAT ACCTGAAGTT GGTAGGGCAG 
AAGTATCTCC ATGATGCGCT AGGGGAGTTT ATCAAGGCTT TGTATGAGTC AGATGAAAAC 
TGTGAAGTGG ACCCCAGCAA ATGTTCATCC AGTGAATTAA CAGACCATCA GAGCAACCTG 
AAAATGTGTT GTGAGCTGGC CTTCTGCAAG ATTATCAATT CCTACTGCGT GTTCCCTCGT 
GAGCTGAAGG AGGTGTTTGC ATCTTGGAAG CAGCAGTGCC TGAGCAGGGG CAAGCAGGAC 
ATCAGCGAGC GGCTGATCAG TGCCTCCCTG TTCCTCCGCT TCCTCTGCCC CGCCATCATG 
TCCCCCAGCC TCTTCAGCCT CATGCAGGAG TATCCTGACG ACCGGACATC GCGCACGCTC 
ACACTCATTG CTAAAGTCAT TCAGAATCTC GCCAATTTTG CTAAGTTTGG TAACAAGGAA 
GAGTACATGG CTTTCATGAA CGACTTTCTG GAACATGAGT GGGGGGGAAT GAAGCGATTT 
CTCCTGGAGA TCTCTAATCC AGATACCATC TCAAACATGC CTGGATTTGA GGGCTATATC 
GACCTGGGCA GAGAGCTCTC AGTTTTGCAT TCCCTCTTAT GGGAGGTTGT GTCCCAACTT 
GATAAGGCGA CCGTGGCAAA ACTGGGACCT CTTCCTCGTA TTCTTGCCGA CATCACCAAA 
TCAATGACCA ACCCTACGCC AATACAGCAG CAGCTGAGGC GTTTCACTGA GCACAGCTCT 
AGTCCAAACG TCAGTGGCAG TCTCTCCTCA GGGCTTCAGA AGATATTCGA GGACCCCACT 
GATGGTGACT TGCATAAACT GAAGTCTCCA ACCCAGGAAA ACATAGATGG CTATTTCAGG 
GGCAAGACCT TGCTGTTGGT TCAGCAGGCG TCCACCCAGA GCATGACTTA CTCAGATAAG 
GACGAAAGAG AGAGCATCTT GCCCAACGGA CGTAGCATCT CCCTCATGGA CCTGCAGGAT 
CCCCATACCG CTCACAGCGA CCACGCTTCC ATGATGCACG ACGTGCCTTT GCGCTTGGCC 
GGCAGCCAGC TCTCCATCAC CCAGGTGGCC AACATCAAGC AGCTGTGGGA GACCCAGAGC 
ACGCCCCAAA GCGCTCCCCA GGTGAGGAGG CCGCTGCACC CGGCTTTGAA CCAGCAGGGC 
AGCCTCCAGC CTCTGTCATT CCAGAACCCG GTGTACCACC TCAACAACCC ACCTGCGCCG 
TTGCCCAAGG CCTCCGTGGA CTCCAGCCTG GAGAACCTGA GCACCGCCAG CTCCCAGAGC 
CGAAGCAACA GCGAAGACTT CAAGCTCAGC GGGCCCAGCA ACAGCAGCAT GGAGGACTTC 
ACCAAGCGCA GCACGCAGAG CGAGGACTTC TCTAGGCGGC ACACGGTCCC GGACAAGCAC 
GTCCCCATCG CGTTGCCCCG CCAGAACAGC AGCAGCCAGG CACAGATCCG CAAAATGGAC 
CAGGCCGGGT TAGGGGCCAG GGCCAAAGCG CCGCAGTCGC TGCCGCACAG CGCGTCCCTG 
CGCAGCACGG GCAGCATGTC GGCCGTGTCG GGGGCCGTGG TGACTGAGCC CGTCCAGAAC 
GGCAGCCGCT CCCGGCAGCA GTCGTCCTCC TCCAGGGAGA GCCCTGTGCC GAAGGTGAGG 
GCCATTCAGA GGCAGCAAAC GCAGCAGGTC CAGTCTCCTG TGGACTCAGC CACAATGTCA 
CCAGTGGAAA GGACAGCAGC GTGGGTTCTC AATAACGGGC AGTACGAGGA GGTCGAGGAG 
GACACAGAGC AGAACCCAGA TGAAGCCAAG CATGCTGAGA AGTACGAACA AGAGATAGCA 
AAGCTGAAGG AACGCTTGAA AGTGTCGAGC CGCCGGCTGG AGGAGTACGA GCGGCGGCTC 
CTGGTGCAGG AGCAGCAAAT GCAGAAGCTG CTGATGGAGT ACAAGTCCAG GCTGGAGGAC 
AGCGAGGAGA GACTGCGCAG GCAACAGGAG GAGAAGGACA GCCAGATGAA AAGCATTATC 
AGCAGGCTCA TGGCAGTTGA AGAGGAACTG AAGAAAGATC ATGCAGAGAT GCAAGCTGTC 
ATCGATGCAA AGCAGAAAAT TATTGATGCA CAGGAGAAGC GGATCGTGTC CCTGGACTCT 
GCCAACACGC GGCTGATGAG TGCCCTGACG CAGGTGAAGG AGCGCTACAG CATGCAAGTC 
CGCAATGGCA TCTCCCCCAC CAACCCCACC AAGCTTTCCA TCACGGAGAA TGGTGAATTC 
AAAAACAGCA GCTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	RASAL2
Entrez Gene ID	429069
Full Name	RAS protein activator like 2
Synonyms	SYNGAP1
General protein information	Preferred Names RAS protein activator like 2 Names ras GTPase-activating protein nGAP synaptic Ras GTPase activating protein 1
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

Danio rerio (zebrafish)	rasal2	XP_001332688.5
Xenopus tropicalis (tropical clawed frog)	rasal2	XP_004914087.1
Homo sapiens (human)	RASAL2	NP_733793.2
Gallus gallus (chicken)	RASAL2	XP_004943302.1
Canis lupus familiaris (dog)	RASAL2	XP_005622535.1
Bos taurus (cattle)	RASAL2	XP_005217296.1
Pan troglodytes (chimpanzee)	RASAL2	XP_003949696.1
Mus musculus (house mouse)	Rasal2	NP_808312.3
	RASAL2	XP_002802086.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

RASAL2 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

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