rasal2 gene cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

mRNA	Protein	Name
XM_004914030.4	XP_004914087.1	ras GTPase-activating protein nGAP isoform X1
XM_002937360.5	XP_002937406.1	ras GTPase-activating protein nGAP isoform X2
XM_031900971.1	XP_031756831.1	ras GTPase-activating protein nGAP isoform X3
XM_031900974.1	XP_031756834.1	ras GTPase-activating protein nGAP isoform X5
XM_004914031.4	XP_004914088.1	ras GTPase-activating protein nGAP isoform X6
XM_004914030.3	XP_004914087.1	ras GTPase-activating protein nGAP isoform X1
XM_002937360.4	XP_002937406.1	ras GTPase-activating protein nGAP isoform X2
XM_004914031.3	XP_004914088.1	ras GTPase-activating protein nGAP isoform X3
XM_004914032.3	XP_004914089.1	ras GTPase-activating protein nGAP isoform X4
XM_031900972.1	XP_031756832.1	ras GTPase-activating protein nGAP isoform X4
XM_004914032.4	XP_004914089.1	ras GTPase-activating protein nGAP isoform X7

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa57217	XM_004914030.4 Latest version!	Xenopus tropicalis RAS protein activator like 2 (rasal2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa57218	XM_002937360.5 Latest version!	Xenopus tropicalis RAS protein activator like 2 (rasal2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa57219	XM_031900971.1 Latest version!	Xenopus tropicalis RAS protein activator like 2 (rasal2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa57220	XM_031900972.1 Latest version!	Xenopus tropicalis RAS protein activator like 2 (rasal2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa57221	XM_031900974.1 Latest version!	Xenopus tropicalis RAS protein activator like 2 (rasal2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa57222	XM_004914031.4 Latest version!	Xenopus tropicalis RAS protein activator like 2 (rasal2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa57223	XM_004914032.4 Latest version!	Xenopus tropicalis RAS protein activator like 2 (rasal2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa09553	XM_004914030.3 Latest version!	Xenopus tropicalis RAS protein activator like 2 (rasal2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa09554	XM_002937360.4 Latest version!	Xenopus tropicalis RAS protein activator like 2 (rasal2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa09555	XM_004914031.3 Latest version!	Xenopus tropicalis RAS protein activator like 2 (rasal2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa09556	XM_004914032.3 Latest version!	Xenopus tropicalis RAS protein activator like 2 (rasal2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

rasal2 ( XM_004914030.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004914087.1 Xenopus tropicalis RAS protein activator like 2 (rasal2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa57217
Clone ID Related Accession (Same CDS sequence)	XM_004914030.4
Accession Version	XM_004914030.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3840bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ras GTPase-activating protein nGAP isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004914030.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCATGA TGGATTTATC CCCCGGCGGG GATTCGGTAA CATCCGGACA TGACTTCTTC 
CAGGACAGAG ATTCCCTCCC AGAGGATTTG GACTCTCCCG GGGGAGTGGG GGGGATTCTG 
GACAGGATCC TAATGGAGTC TGTGTGCCAG CAGCAGGGAT GGGTGCGAGT ATACGATGTG 
AAAGGCCCGC CTACACACAG GCTGTCGTGT GGTCAGTCGC CCTACACTGA AAGCACAACA 
TGGGAGCGCA AGTACTGTAT CCTAACAGAA AGCCAACTGG TGCTCCTTAA CCGTGACGAT 
GAGATGCCTG GGGAAACTGC ACAGGACCCA CCTCCAGAAT CCAGCAAAAG CAGAAGCCTG 
CGTAGGACTG TGAGTGTCCC GTCAGAAGGG CAGTTTCCAG ACTACCCCCC GGAGGGTGCT 
GCCAAGCTGG AGGTCCCAGC TGAGAGGTCA CCCCGAAGGA GGAGTATCTC AGGGACTAGC 
ACGTCAGACA AAACCAATGC TATGGATCCT GCAAATACCT CCCCCTTTAA AGTCTCAGGC 
TTCTTTAGTA AACGGTTAAA AGGCTCTATT AAGAGAACGA AGAGTCAATC AAAGCTGGAC 
CGGAATACAA GTTTTCGCCT GCCTTCTCTT CGTCCTGCTG AGAGTGACAG GTCACGTGGG 
TTGCCTAAAC TAAAAGAATC TCGTTCCCAT GAATCTTTGC TGAGCCCTGG TAGTGCAGTT 
GAGGCTCTGG ATTTGGGAAC TGAAGATAAA ATCCTAGTAA AACCGCTCCA CAGCAGCATA 
TTGGGACAAG ATTTCTGCTT TGAGGTCACC TATTCCAGTG GCAGTAAATG TTTCAGCTGC 
TCATCTGCAG CAGAGAGAGA TAAGTGGATG GAAAATCTTC GTCGAACTGT TCAACCAAAC 
AAGGATAACT GTCGCCGAGC AGAGAATGTA CTACGGCTGT GGATCATTGA AGCCAAGGAC 
CTGGCTCCCA AAAAGAAGTA CTTCTGTGAA CTTTGTCTGG ACGATACTCT CTTTGCTCGA 
ACCACAAGCA AGACGAAAGC GGACAATATT TTTTGGGGGG AATATTTTGA GTTTTATGGT 
TTACCACCCC TGCACAACAT CACAGTTCAT ATTTACAAGG ATGTTGAAAA GAAGAAAAAA 
AAAGATAAAA ACAATTATGT TGGTTTAGTG AATATTCCTA TGATTAGTGT AACTGGGAGG 
CAGTTTGTGG AGAAATGGTA CCCAGTTAGC ACCCCCACTG CAAACAAAGG CAAGACTGTT 
GGTCCTTCCA TACGTATAAA GTCCCGTTAC CAAACTATTA CCATTTTACC CATGGAGCAA 
TATAAGGAAT TTGCAGAATT TATCACAATT AATTACACTA TGCTATGCTC AGTCCTGGAA 
CCAGTCATTA GTGTCAGAAA TAAGGAAGAG ATGGCATGTG CTTTAGTGCA CATCCTGCAG 
AGTACTGGCA GAGCCAAGGA CTTTCTAACT GATCTGGTCA TGTCAGAGGT AGATCGTTGT 
GGGGAGCACG ATGTCTTGAT CTTTAGGGAA AATACACTTG CCACTAAAGC CATTGAGGAG 
TATCTAAAGC TGGTTGGACA GAAGTACCTT CATGATGCTC TTGGGGAGTT TATCAAGGCA 
CTGTATGAGT CGGATGAGAA CTGTGAGGTG GATCCCAGCA AATGTTCCTC AAGTGAGCTG 
TCCGACCACC AGAGCAACTT AAAAATGTGC TGCGAGCTGG CCTTCTGCAA GATAATCAAT 
TCATATTGTG TTTTTCCCAG AGAGCTGAAG GAAGTGTTTG CTTCTTGGAA GCAGCAATGC 
ATAAATCGGG GAAAGCAGGA TATCAGCGAA CGACTGATCA GCGCATCACT CTTCCTTCGC 
TTTCTTTGTC CTGCAATTAT GTCTCCCAGC CTCTTTGGGC TAATGCAGGA GTATCCTGAT 
GATCGCACCT CCAGAACTCT CACACTCATT GCCAAGGTCA TCCAGAATCT TGCCAACTTT 
GCCAAGTTTG GTAATAAAGA AGAATACATG GCCTTTATGA ATGACTTTTT GGAGCATGAG 
TGGGGTGGAA TGAAACGATT CCTACAAGAG ATCTCCAATC CAGACACCAT TTCAAACACC 
CCCGGATTTG AGGGTTACAT AGACCTTGGC CGAGAGCTCT CCGTACTGCA CAGTCTTTTG 
TGGGAGGTGG TGTCACAGCT AGATAAGGGT GAAAATTCCT TCCTACAGGC GACCGTTGAG 
AAATTGGGAC CCCTTCCACG CATTCTTGCT GACATCACCA AGTCACTGAC CAACCCTACT 
CCAATCCAAC AGCAGCTAAG ACGTTTCAGT GAACATAGCT CCACACCCAA TGTAAGTGGC 
AGCCTTTCAT CTGGACTTCA GAAGATTTTT GAGGATCCAG CAGACAGTGA TTTGCAGAGG 
TTCAGGTCTC CAACAATTGA AAACCCAGAA AGCTATTTCA GGGGGAAAAC GCTACTGCTT 
GTCCAGCAGG CATCCACACA GAGTATGTCC TTTTCCGACA AAGACGAAAG AGAGAATGTC 
TTGCCCAATG GGCGTAGTGT GTCTTTAATG GATCTGCAAG ACTCCAATGT TAGTCACACT 
GATCGTTCCT CAGTTATCCA AGAACCTCCC TTACGTTTAA CTGGCAGCCA GTTGTCTATT 
GCACATGTAG CTACCATTAA ACAGCTACGG GAAAACCATA GCATGCCTCA GAGTGCCCCA 
CAAGTTAGAA AGCCCTTACA TCCAGCATTG AGCCAGCAAA GCAGCCTGCA GCCTCTGTCC 
TTCCAAAATC CTGTGTATCA TCTGAACAAC CCACCACCGA CAATGCCCAA AGCTTCTGTG 
GACTCCAGCC TTGAAAATTT AAGCACTGCC AGCTCTAGAA GCCAGAGTAA TAGTGAAGAG 
TTCATTCTCA GTGGGCCCAG TAACAGTAGC ATGGAAGATT TCACAAAGAG GAGCACTCAG 
AGTGAGGACT TTACAAGAAG ACACACCATG CCTGAGAGAC CTATTCCCAT AGCACTGCCA 
AGGCAAAACA GCACTGGACA AGCCCAGATC CGTAAAACCG ACCAGGCTGC TCTTGGGGCT 
CGAGCAAAAG CCCCCCAGTC ATTACCACAC AGTGCCTCAC TGCGGAGCGC AGGGAGCATG 
TCTGGGATCT CCAATAATAT GCTTGTTGAA CCAGTGCAGA ATGGAAATAG GTCCAGACAG 
CAATCTACAT CATCTCGTGA AAGCCCTGTA CCCAAACTAA GAGCAATTCA CCGTCAGCAG 
ACACAGCAGG TGCAGTCACC TGTGGACTCC GCTACAATGT CTCCAGTAGA GCGGACTGCA 
GCCTGGGTTC TTAATAACGG TCAATATGAA GATGAAGAAG AAACTGTAGA CCAAAACAAG 
GATGAATCTA AACCTGCAGA GAAGTATGAA CAAGAGATTG CAAAGCTGAA AGAACGATTG 
AAAATTTCAA GTCGGCGCCT GGAGGAATAT GAACGGCGTC TTTTGGTGCA AGAGCAGCAA 
ATGCAGAAGC TCTTAATGGA GTACAAATCT CGACTGGAGG ATAGCGAGGA GAGGTTGCGC 
CGTCAGCAGG AAGAGAAGGA CAGCCAAATG AAAAGTGTCA TCAGCAGGTT AATAGCTGTA 
GAAGAGGAGC TGAAAAAAGA CCATGCTGAG ATGCAGGCTG TCATTGATGC AAAGCAAAAA 
ATTATTGATG CACAGGAGAA GAGGATCATA TCACTGGATG CAGCCAACAC GCGGCTAATG 
AGTGCCCTCA CACAGGTCAA GGAACGCTAT AACTCCCAGG TGCGCAATGG AATCTCTCCG 
ACCAACCCTA CCAAGCTGTC AATCACTGAA AATGGAGAAT TTAAGAACAG CAGTTGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004914087.1
CDS	659..4498
Translation	MLMMDLSPGGDSVTSGHDFFQDRDSLPEDLDSPGGVGGILDRILMESVCQQQGWVRVYDVKGPPTHRLSCGQSPYTESTTWERKYCILTESQLVLLNRDDEMPGETAQDPPPESSKSRSLRRTVSVPSEGQFPDYPPEGAAKLEVPAERSPRRRSISGTSTSDKTNAMDPANTSPFKVSGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRPAESDRSRGLPKLKESRSHESLLSPGSAVEALDLGTEDKILVKPLHSSILGQDFCFEVTYSSGSKCFSCSSAAERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEYFEFYGLPPLHNITVHIYKDVEKKKKKDKNNYVGLVNIPMISVTGRQFVEKWYPVSTPTANKGKTVGPSIRIKSRYQTITILPMEQYKEFAEFITINYTMLCSVLEPVISVRNKEEMACALVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTLATKAIEEYLKLVGQKYLHDALGEFIKALYESDENCEVDPSKCSSSELSDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCINRGKQDISERLISASLFLRFLCPAIMSPSLFGLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLQEISNPDTISNTPGFEGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVEKLGPLPRILADITKSLTNPTPIQQQLRRFSEHSSTPNVSGSLSSGLQKIFEDPADSDLQRFRSPTIENPESYFRGKTLLLVQQASTQSMSFSDKDERENVLPNGRSVSLMDLQDSNVSHTDRSSVIQEPPLRLTGSQLSIAHVATIKQLRENHSMPQSAPQVRKPLHPALSQQSSLQPLSFQNPVYHLNNPPPTMPKASVDSSLENLSTASSRSQSNSEEFILSGPSNSSMEDFTKRSTQSEDFTRRHTMPERPIPIALPRQNSTGQAQIRKTDQAALGARAKAPQSLPHSASLRSAGSMSGISNNMLVEPVQNGNRSRQQSTSSRESPVPKLRAIHRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEDEEETVDQNKDESKPAEKYEQEIAKLKERLKISSRRLEEYERRLLVQEQQMQKLLMEYKSRLEDSEERLRRQQEEKDSQMKSVISRLIAVEEELKKDHAEMQAVIDAKQKIIDAQEKRIISLDAANTRLMSALTQVKERYNSQVRNGISPTNPTKLSITENGEFKNSSC

Target ORF information:

RefSeq Version	XM_004914030.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis RAS protein activator like 2 (rasal2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004914030.4

ORF Insert Sequence:

ATGCTCATGA TGGATTTATC CCCCGGCGGG GATTCGGTAA CATCCGGACA TGACTTCTTC 
CAGGACAGAG ATTCCCTCCC AGAGGATTTG GACTCTCCCG GGGGAGTGGG GGGGATTCTG 
GACAGGATCC TAATGGAGTC TGTGTGCCAG CAGCAGGGAT GGGTGCGAGT ATACGATGTG 
AAAGGCCCGC CTACACACAG GCTGTCGTGT GGTCAGTCGC CCTACACTGA AAGCACAACA 
TGGGAGCGCA AGTACTGTAT CCTAACAGAA AGCCAACTGG TGCTCCTTAA CCGTGACGAT 
GAGATGCCTG GGGAAACTGC ACAGGACCCA CCTCCAGAAT CCAGCAAAAG CAGAAGCCTG 
CGTAGGACTG TGAGTGTCCC GTCAGAAGGG CAGTTTCCAG ACTACCCCCC GGAGGGTGCT 
GCCAAGCTGG AGGTCCCAGC TGAGAGGTCA CCCCGAAGGA GGAGTATCTC AGGGACTAGC 
ACGTCAGACA AAACCAATGC TATGGATCCT GCAAATACCT CCCCCTTTAA AGTCTCAGGC 
TTCTTTAGTA AACGGTTAAA AGGCTCTATT AAGAGAACGA AGAGTCAATC AAAGCTGGAC 
CGGAATACAA GTTTTCGCCT GCCTTCTCTT CGTCCTGCTG AGAGTGACAG GTCACGTGGG 
TTGCCTAAAC TAAAAGAATC TCGTTCCCAT GAATCTTTGC TGAGCCCTGG TAGTGCAGTT 
GAGGCTCTGG ATTTGGGAAC TGAAGATAAA ATCCTAGTAA AACCGCTCCA CAGCAGCATA 
TTGGGACAAG ATTTCTGCTT TGAGGTCACC TATTCCAGTG GCAGTAAATG TTTCAGCTGC 
TCATCTGCAG CAGAGAGAGA TAAGTGGATG GAAAATCTTC GTCGAACTGT TCAACCAAAC 
AAGGATAACT GTCGCCGAGC AGAGAATGTA CTACGGCTGT GGATCATTGA AGCCAAGGAC 
CTGGCTCCCA AAAAGAAGTA CTTCTGTGAA CTTTGTCTGG ACGATACTCT CTTTGCTCGA 
ACCACAAGCA AGACGAAAGC GGACAATATT TTTTGGGGGG AATATTTTGA GTTTTATGGT 
TTACCACCCC TGCACAACAT CACAGTTCAT ATTTACAAGG ATGTTGAAAA GAAGAAAAAA 
AAAGATAAAA ACAATTATGT TGGTTTAGTG AATATTCCTA TGATTAGTGT AACTGGGAGG 
CAGTTTGTGG AGAAATGGTA CCCAGTTAGC ACCCCCACTG CAAACAAAGG CAAGACTGTT 
GGTCCTTCCA TACGTATAAA GTCCCGTTAC CAAACTATTA CCATTTTACC CATGGAGCAA 
TATAAGGAAT TTGCAGAATT TATCACAATT AATTACACTA TGCTATGCTC AGTCCTGGAA 
CCAGTCATTA GTGTCAGAAA TAAGGAAGAG ATGGCATGTG CTTTAGTGCA CATCCTGCAG 
AGTACTGGCA GAGCCAAGGA CTTTCTAACT GATCTGGTCA TGTCAGAGGT AGATCGTTGT 
GGGGAGCACG ATGTCTTGAT CTTTAGGGAA AATACACTTG CCACTAAAGC CATTGAGGAG 
TATCTAAAGC TGGTTGGACA GAAGTACCTT CATGATGCTC TTGGGGAGTT TATCAAGGCA 
CTGTATGAGT CGGATGAGAA CTGTGAGGTG GATCCCAGCA AATGTTCCTC AAGTGAGCTG 
TCCGACCACC AGAGCAACTT AAAAATGTGC TGCGAGCTGG CCTTCTGCAA GATAATCAAT 
TCATATTGTG TTTTTCCCAG AGAGCTGAAG GAAGTGTTTG CTTCTTGGAA GCAGCAATGC 
ATAAATCGGG GAAAGCAGGA TATCAGCGAA CGACTGATCA GCGCATCACT CTTCCTTCGC 
TTTCTTTGTC CTGCAATTAT GTCTCCCAGC CTCTTTGGGC TAATGCAGGA GTATCCTGAT 
GATCGCACCT CCAGAACTCT CACACTCATT GCCAAGGTCA TCCAGAATCT TGCCAACTTT 
GCCAAGTTTG GTAATAAAGA AGAATACATG GCCTTTATGA ATGACTTTTT GGAGCATGAG 
TGGGGTGGAA TGAAACGATT CCTACAAGAG ATCTCCAATC CAGACACCAT TTCAAACACC 
CCCGGATTTG AGGGTTACAT AGACCTTGGC CGAGAGCTCT CCGTACTGCA CAGTCTTTTG 
TGGGAGGTGG TGTCACAGCT AGATAAGGGT GAAAATTCCT TCCTACAGGC GACCGTTGAG 
AAATTGGGAC CCCTTCCACG CATTCTTGCT GACATCACCA AGTCACTGAC CAACCCTACT 
CCAATCCAAC AGCAGCTAAG ACGTTTCAGT GAACATAGCT CCACACCCAA TGTAAGTGGC 
AGCCTTTCAT CTGGACTTCA GAAGATTTTT GAGGATCCAG CAGACAGTGA TTTGCAGAGG 
TTCAGGTCTC CAACAATTGA AAACCCAGAA AGCTATTTCA GGGGGAAAAC GCTACTGCTT 
GTCCAGCAGG CATCCACACA GAGTATGTCC TTTTCCGACA AAGACGAAAG AGAGAATGTC 
TTGCCCAATG GGCGTAGTGT GTCTTTAATG GATCTGCAAG ACTCCAATGT TAGTCACACT 
GATCGTTCCT CAGTTATCCA AGAACCTCCC TTACGTTTAA CTGGCAGCCA GTTGTCTATT 
GCACATGTAG CTACCATTAA ACAGCTACGG GAAAACCATA GCATGCCTCA GAGTGCCCCA 
CAAGTTAGAA AGCCCTTACA TCCAGCATTG AGCCAGCAAA GCAGCCTGCA GCCTCTGTCC 
TTCCAAAATC CTGTGTATCA TCTGAACAAC CCACCACCGA CAATGCCCAA AGCTTCTGTG 
GACTCCAGCC TTGAAAATTT AAGCACTGCC AGCTCTAGAA GCCAGAGTAA TAGTGAAGAG 
TTCATTCTCA GTGGGCCCAG TAACAGTAGC ATGGAAGATT TCACAAAGAG GAGCACTCAG 
AGTGAGGACT TTACAAGAAG ACACACCATG CCTGAGAGAC CTATTCCCAT AGCACTGCCA 
AGGCAAAACA GCACTGGACA AGCCCAGATC CGTAAAACCG ACCAGGCTGC TCTTGGGGCT 
CGAGCAAAAG CCCCCCAGTC ATTACCACAC AGTGCCTCAC TGCGGAGCGC AGGGAGCATG 
TCTGGGATCT CCAATAATAT GCTTGTTGAA CCAGTGCAGA ATGGAAATAG GTCCAGACAG 
CAATCTACAT CATCTCGTGA AAGCCCTGTA CCCAAACTAA GAGCAATTCA CCGTCAGCAG 
ACACAGCAGG TGCAGTCACC TGTGGACTCC GCTACAATGT CTCCAGTAGA GCGGACTGCA 
GCCTGGGTTC TTAATAACGG TCAATATGAA GATGAAGAAG AAACTGTAGA CCAAAACAAG 
GATGAATCTA AACCTGCAGA GAAGTATGAA CAAGAGATTG CAAAGCTGAA AGAACGATTG 
AAAATTTCAA GTCGGCGCCT GGAGGAATAT GAACGGCGTC TTTTGGTGCA AGAGCAGCAA 
ATGCAGAAGC TCTTAATGGA GTACAAATCT CGACTGGAGG ATAGCGAGGA GAGGTTGCGC 
CGTCAGCAGG AAGAGAAGGA CAGCCAAATG AAAAGTGTCA TCAGCAGGTT AATAGCTGTA 
GAAGAGGAGC TGAAAAAAGA CCATGCTGAG ATGCAGGCTG TCATTGATGC AAAGCAAAAA 
ATTATTGATG CACAGGAGAA GAGGATCATA TCACTGGATG CAGCCAACAC GCGGCTAATG 
AGTGCCCTCA CACAGGTCAA GGAACGCTAT AACTCCCAGG TGCGCAATGG AATCTCTCCG 
ACCAACCCTA CCAAGCTGTC AATCACTGAA AATGGAGAAT TTAAGAACAG CAGTTGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rasal2 ( XM_002937360.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002937406.1 Xenopus tropicalis RAS protein activator like 2 (rasal2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa57218
Clone ID Related Accession (Same CDS sequence)	XM_002937360.5
Accession Version	XM_002937360.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3819bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ras GTPase-activating protein nGAP isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002937360.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCATGA TGGATTTATC CCCCGGCGGG GATTCGGTAA CATCCGGACA TGACTTCTTC 
CAGGACAGAG ATTCCCTCCC AGAGGATTTG GACTCTCCCG GGGGAGTGGG GGGGATTCTG 
GACAGGATCC TAATGGAGTC TGTGTGCCAG CAGCAGGGAT GGGTGCGAGT ATACGATGTG 
AAAGGCCCGC CTACACACAG GCTGTCGTGT GGTCAGTCGC CCTACACTGA AAGCACAACA 
TGGGAGCGCA AGTACTGTAT CCTAACAGAA AGCCAACTGG TGCTCCTTAA CCGTGACGAT 
GAGATGCCTG GGGAAACTGC ACAGGACCCA CCTCCAGAAT CCAGCAAAAG CAGAAGCCTG 
CGTAGGACTG TGAGTGTCCC GTCAGAAGGG CAGTTTCCAG ACTACCCCCC GGAGGGTGCT 
GCCAAGCTGG AGGTCCCAGC TGAGAGGTCA CCCCGAAGGA GGAGTATCTC AGGGACTAGC 
ACGTCAGACA AAACCAATGC TATGGATCCT GCAAATACCT CCCCCTTTAA AGTCTCAGGC 
TTCTTTAGTA AACGGTTAAA AGGCTCTATT AAGAGAACGA AGAGTCAATC AAAGCTGGAC 
CGGAATACAA GTTTTCGCCT GCCTTCTCTT CGTCCTGCTG AGAGTGACAG GTCACGTGGG 
TTGCCTAAAC TAAAAGAATC TCGTTCCCAT GAATCTTTGC TGAGCCCTGG TAGTGCAGTT 
GAGGCTCTGG ATTTGGGAAC TGAAGATAAA ATCCTAGTAA AACCGCTCCA CAGCAGCATA 
TTGGGACAAG ATTTCTGCTT TGAGGTCACC TATTCCAGTG GCAGTAAATG TTTCAGCTGC 
TCATCTGCAG CAGAGAGAGA TAAGTGGATG GAAAATCTTC GTCGAACTGT TCAACCAAAC 
AAGGATAACT GTCGCCGAGC AGAGAATGTA CTACGGCTGT GGATCATTGA AGCCAAGGAC 
CTGGCTCCCA AAAAGAAGTA CTTCTGTGAA CTTTGTCTGG ACGATACTCT CTTTGCTCGA 
ACCACAAGCA AGACGAAAGC GGACAATATT TTTTGGGGGG AATATTTTGA GTTTTATGGT 
TTACCACCCC TGCACAACAT CACAGTTCAT ATTTACAAGG ATGTTGAAAA GAAGAAAAAA 
AAAGATAAAA ACAATTATGT TGGTTTAGTG AATATTCCTA TGATTAGTGT AACTGGGAGG 
CAGTTTGTGG AGAAATGGTA CCCAGTTAGC ACCCCCACTG CAAACAAAGG CAAGACTGTT 
GGTCCTTCCA TACGTATAAA GTCCCGTTAC CAAACTATTA CCATTTTACC CATGGAGCAA 
TATAAGGAAT TTGCAGAATT TATCACAATT AATTACACTA TGCTATGCTC AGTCCTGGAA 
CCAGTCATTA GTGTCAGAAA TAAGGAAGAG ATGGCATGTG CTTTAGTGCA CATCCTGCAG 
AGTACTGGCA GAGCCAAGGA CTTTCTAACT GATCTGGTCA TGTCAGAGGT AGATCGTTGT 
GGGGAGCACG ATGTCTTGAT CTTTAGGGAA AATACACTTG CCACTAAAGC CATTGAGGAG 
TATCTAAAGC TGGTTGGACA GAAGTACCTT CATGATGCTC TTGGGGAGTT TATCAAGGCA 
CTGTATGAGT CGGATGAGAA CTGTGAGGTG GATCCCAGCA AATGTTCCTC AAGTGAGCTG 
TCCGACCACC AGAGCAACTT AAAAATGTGC TGCGAGCTGG CCTTCTGCAA GATAATCAAT 
TCATATTGTG TTTTTCCCAG AGAGCTGAAG GAAGTGTTTG CTTCTTGGAA GCAGCAATGC 
ATAAATCGGG GAAAGCAGGA TATCAGCGAA CGACTGATCA GCGCATCACT CTTCCTTCGC 
TTTCTTTGTC CTGCAATTAT GTCTCCCAGC CTCTTTGGGC TAATGCAGGA GTATCCTGAT 
GATCGCACCT CCAGAACTCT CACACTCATT GCCAAGGTCA TCCAGAATCT TGCCAACTTT 
GCCAAGTTTG GTAATAAAGA AGAATACATG GCCTTTATGA ATGACTTTTT GGAGCATGAG 
TGGGGTGGAA TGAAACGATT CCTACAAGAG ATCTCCAATC CAGACACCAT TTCAAACACC 
CCCGGATTTG AGGGTTACAT AGACCTTGGC CGAGAGCTCT CCGTACTGCA CAGTCTTTTG 
TGGGAGGTGG TGTCACAGCT AGATAAGGCG ACCGTTGAGA AATTGGGACC CCTTCCACGC 
ATTCTTGCTG ACATCACCAA GTCACTGACC AACCCTACTC CAATCCAACA GCAGCTAAGA 
CGTTTCAGTG AACATAGCTC CACACCCAAT GTAAGTGGCA GCCTTTCATC TGGACTTCAG 
AAGATTTTTG AGGATCCAGC AGACAGTGAT TTGCAGAGGT TCAGGTCTCC AACAATTGAA 
AACCCAGAAA GCTATTTCAG GGGGAAAACG CTACTGCTTG TCCAGCAGGC ATCCACACAG 
AGTATGTCCT TTTCCGACAA AGACGAAAGA GAGAATGTCT TGCCCAATGG GCGTAGTGTG 
TCTTTAATGG ATCTGCAAGA CTCCAATGTT AGTCACACTG ATCGTTCCTC AGTTATCCAA 
GAACCTCCCT TACGTTTAAC TGGCAGCCAG TTGTCTATTG CACATGTAGC TACCATTAAA 
CAGCTACGGG AAAACCATAG CATGCCTCAG AGTGCCCCAC AAGTTAGAAA GCCCTTACAT 
CCAGCATTGA GCCAGCAAAG CAGCCTGCAG CCTCTGTCCT TCCAAAATCC TGTGTATCAT 
CTGAACAACC CACCACCGAC AATGCCCAAA GCTTCTGTGG ACTCCAGCCT TGAAAATTTA 
AGCACTGCCA GCTCTAGAAG CCAGAGTAAT AGTGAAGAGT TCATTCTCAG TGGGCCCAGT 
AACAGTAGCA TGGAAGATTT CACAAAGAGG AGCACTCAGA GTGAGGACTT TACAAGAAGA 
CACACCATGC CTGAGAGACC TATTCCCATA GCACTGCCAA GGCAAAACAG CACTGGACAA 
GCCCAGATCC GTAAAACCGA CCAGGCTGCT CTTGGGGCTC GAGCAAAAGC CCCCCAGTCA 
TTACCACACA GTGCCTCACT GCGGAGCGCA GGGAGCATGT CTGGGATCTC CAATAATATG 
CTTGTTGAAC CAGTGCAGAA TGGAAATAGG TCCAGACAGC AATCTACATC ATCTCGTGAA 
AGCCCTGTAC CCAAACTAAG AGCAATTCAC CGTCAGCAGA CACAGCAGGT GCAGTCACCT 
GTGGACTCCG CTACAATGTC TCCAGTAGAG CGGACTGCAG CCTGGGTTCT TAATAACGGT 
CAATATGAAG ATGAAGAAGA AACTGTAGAC CAAAACAAGG ATGAATCTAA ACCTGCAGAG 
AAGTATGAAC AAGAGATTGC AAAGCTGAAA GAACGATTGA AAATTTCAAG TCGGCGCCTG 
GAGGAATATG AACGGCGTCT TTTGGTGCAA GAGCAGCAAA TGCAGAAGCT CTTAATGGAG 
TACAAATCTC GACTGGAGGA TAGCGAGGAG AGGTTGCGCC GTCAGCAGGA AGAGAAGGAC 
AGCCAAATGA AAAGTGTCAT CAGCAGGTTA ATAGCTGTAG AAGAGGAGCT GAAAAAAGAC 
CATGCTGAGA TGCAGGCTGT CATTGATGCA AAGCAAAAAA TTATTGATGC ACAGGAGAAG 
AGGATCATAT CACTGGATGC AGCCAACACG CGGCTAATGA GTGCCCTCAC ACAGGTCAAG 
GAACGCTATA ACTCCCAGGT GCGCAATGGA ATCTCTCCGA CCAACCCTAC CAAGCTGTCA 
ATCACTGAAA ATGGAGAATT TAAGAACAGC AGTTGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002937406.1
CDS	659..4477
Translation	MLMMDLSPGGDSVTSGHDFFQDRDSLPEDLDSPGGVGGILDRILMESVCQQQGWVRVYDVKGPPTHRLSCGQSPYTESTTWERKYCILTESQLVLLNRDDEMPGETAQDPPPESSKSRSLRRTVSVPSEGQFPDYPPEGAAKLEVPAERSPRRRSISGTSTSDKTNAMDPANTSPFKVSGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRPAESDRSRGLPKLKESRSHESLLSPGSAVEALDLGTEDKILVKPLHSSILGQDFCFEVTYSSGSKCFSCSSAAERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEYFEFYGLPPLHNITVHIYKDVEKKKKKDKNNYVGLVNIPMISVTGRQFVEKWYPVSTPTANKGKTVGPSIRIKSRYQTITILPMEQYKEFAEFITINYTMLCSVLEPVISVRNKEEMACALVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTLATKAIEEYLKLVGQKYLHDALGEFIKALYESDENCEVDPSKCSSSELSDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCINRGKQDISERLISASLFLRFLCPAIMSPSLFGLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLQEISNPDTISNTPGFEGYIDLGRELSVLHSLLWEVVSQLDKATVEKLGPLPRILADITKSLTNPTPIQQQLRRFSEHSSTPNVSGSLSSGLQKIFEDPADSDLQRFRSPTIENPESYFRGKTLLLVQQASTQSMSFSDKDERENVLPNGRSVSLMDLQDSNVSHTDRSSVIQEPPLRLTGSQLSIAHVATIKQLRENHSMPQSAPQVRKPLHPALSQQSSLQPLSFQNPVYHLNNPPPTMPKASVDSSLENLSTASSRSQSNSEEFILSGPSNSSMEDFTKRSTQSEDFTRRHTMPERPIPIALPRQNSTGQAQIRKTDQAALGARAKAPQSLPHSASLRSAGSMSGISNNMLVEPVQNGNRSRQQSTSSRESPVPKLRAIHRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEDEEETVDQNKDESKPAEKYEQEIAKLKERLKISSRRLEEYERRLLVQEQQMQKLLMEYKSRLEDSEERLRRQQEEKDSQMKSVISRLIAVEEELKKDHAEMQAVIDAKQKIIDAQEKRIISLDAANTRLMSALTQVKERYNSQVRNGISPTNPTKLSITENGEFKNSSC

Target ORF information:

RefSeq Version	XM_002937360.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis RAS protein activator like 2 (rasal2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002937360.5

ORF Insert Sequence:

ATGCTCATGA TGGATTTATC CCCCGGCGGG GATTCGGTAA CATCCGGACA TGACTTCTTC 
CAGGACAGAG ATTCCCTCCC AGAGGATTTG GACTCTCCCG GGGGAGTGGG GGGGATTCTG 
GACAGGATCC TAATGGAGTC TGTGTGCCAG CAGCAGGGAT GGGTGCGAGT ATACGATGTG 
AAAGGCCCGC CTACACACAG GCTGTCGTGT GGTCAGTCGC CCTACACTGA AAGCACAACA 
TGGGAGCGCA AGTACTGTAT CCTAACAGAA AGCCAACTGG TGCTCCTTAA CCGTGACGAT 
GAGATGCCTG GGGAAACTGC ACAGGACCCA CCTCCAGAAT CCAGCAAAAG CAGAAGCCTG 
CGTAGGACTG TGAGTGTCCC GTCAGAAGGG CAGTTTCCAG ACTACCCCCC GGAGGGTGCT 
GCCAAGCTGG AGGTCCCAGC TGAGAGGTCA CCCCGAAGGA GGAGTATCTC AGGGACTAGC 
ACGTCAGACA AAACCAATGC TATGGATCCT GCAAATACCT CCCCCTTTAA AGTCTCAGGC 
TTCTTTAGTA AACGGTTAAA AGGCTCTATT AAGAGAACGA AGAGTCAATC AAAGCTGGAC 
CGGAATACAA GTTTTCGCCT GCCTTCTCTT CGTCCTGCTG AGAGTGACAG GTCACGTGGG 
TTGCCTAAAC TAAAAGAATC TCGTTCCCAT GAATCTTTGC TGAGCCCTGG TAGTGCAGTT 
GAGGCTCTGG ATTTGGGAAC TGAAGATAAA ATCCTAGTAA AACCGCTCCA CAGCAGCATA 
TTGGGACAAG ATTTCTGCTT TGAGGTCACC TATTCCAGTG GCAGTAAATG TTTCAGCTGC 
TCATCTGCAG CAGAGAGAGA TAAGTGGATG GAAAATCTTC GTCGAACTGT TCAACCAAAC 
AAGGATAACT GTCGCCGAGC AGAGAATGTA CTACGGCTGT GGATCATTGA AGCCAAGGAC 
CTGGCTCCCA AAAAGAAGTA CTTCTGTGAA CTTTGTCTGG ACGATACTCT CTTTGCTCGA 
ACCACAAGCA AGACGAAAGC GGACAATATT TTTTGGGGGG AATATTTTGA GTTTTATGGT 
TTACCACCCC TGCACAACAT CACAGTTCAT ATTTACAAGG ATGTTGAAAA GAAGAAAAAA 
AAAGATAAAA ACAATTATGT TGGTTTAGTG AATATTCCTA TGATTAGTGT AACTGGGAGG 
CAGTTTGTGG AGAAATGGTA CCCAGTTAGC ACCCCCACTG CAAACAAAGG CAAGACTGTT 
GGTCCTTCCA TACGTATAAA GTCCCGTTAC CAAACTATTA CCATTTTACC CATGGAGCAA 
TATAAGGAAT TTGCAGAATT TATCACAATT AATTACACTA TGCTATGCTC AGTCCTGGAA 
CCAGTCATTA GTGTCAGAAA TAAGGAAGAG ATGGCATGTG CTTTAGTGCA CATCCTGCAG 
AGTACTGGCA GAGCCAAGGA CTTTCTAACT GATCTGGTCA TGTCAGAGGT AGATCGTTGT 
GGGGAGCACG ATGTCTTGAT CTTTAGGGAA AATACACTTG CCACTAAAGC CATTGAGGAG 
TATCTAAAGC TGGTTGGACA GAAGTACCTT CATGATGCTC TTGGGGAGTT TATCAAGGCA 
CTGTATGAGT CGGATGAGAA CTGTGAGGTG GATCCCAGCA AATGTTCCTC AAGTGAGCTG 
TCCGACCACC AGAGCAACTT AAAAATGTGC TGCGAGCTGG CCTTCTGCAA GATAATCAAT 
TCATATTGTG TTTTTCCCAG AGAGCTGAAG GAAGTGTTTG CTTCTTGGAA GCAGCAATGC 
ATAAATCGGG GAAAGCAGGA TATCAGCGAA CGACTGATCA GCGCATCACT CTTCCTTCGC 
TTTCTTTGTC CTGCAATTAT GTCTCCCAGC CTCTTTGGGC TAATGCAGGA GTATCCTGAT 
GATCGCACCT CCAGAACTCT CACACTCATT GCCAAGGTCA TCCAGAATCT TGCCAACTTT 
GCCAAGTTTG GTAATAAAGA AGAATACATG GCCTTTATGA ATGACTTTTT GGAGCATGAG 
TGGGGTGGAA TGAAACGATT CCTACAAGAG ATCTCCAATC CAGACACCAT TTCAAACACC 
CCCGGATTTG AGGGTTACAT AGACCTTGGC CGAGAGCTCT CCGTACTGCA CAGTCTTTTG 
TGGGAGGTGG TGTCACAGCT AGATAAGGCG ACCGTTGAGA AATTGGGACC CCTTCCACGC 
ATTCTTGCTG ACATCACCAA GTCACTGACC AACCCTACTC CAATCCAACA GCAGCTAAGA 
CGTTTCAGTG AACATAGCTC CACACCCAAT GTAAGTGGCA GCCTTTCATC TGGACTTCAG 
AAGATTTTTG AGGATCCAGC AGACAGTGAT TTGCAGAGGT TCAGGTCTCC AACAATTGAA 
AACCCAGAAA GCTATTTCAG GGGGAAAACG CTACTGCTTG TCCAGCAGGC ATCCACACAG 
AGTATGTCCT TTTCCGACAA AGACGAAAGA GAGAATGTCT TGCCCAATGG GCGTAGTGTG 
TCTTTAATGG ATCTGCAAGA CTCCAATGTT AGTCACACTG ATCGTTCCTC AGTTATCCAA 
GAACCTCCCT TACGTTTAAC TGGCAGCCAG TTGTCTATTG CACATGTAGC TACCATTAAA 
CAGCTACGGG AAAACCATAG CATGCCTCAG AGTGCCCCAC AAGTTAGAAA GCCCTTACAT 
CCAGCATTGA GCCAGCAAAG CAGCCTGCAG CCTCTGTCCT TCCAAAATCC TGTGTATCAT 
CTGAACAACC CACCACCGAC AATGCCCAAA GCTTCTGTGG ACTCCAGCCT TGAAAATTTA 
AGCACTGCCA GCTCTAGAAG CCAGAGTAAT AGTGAAGAGT TCATTCTCAG TGGGCCCAGT 
AACAGTAGCA TGGAAGATTT CACAAAGAGG AGCACTCAGA GTGAGGACTT TACAAGAAGA 
CACACCATGC CTGAGAGACC TATTCCCATA GCACTGCCAA GGCAAAACAG CACTGGACAA 
GCCCAGATCC GTAAAACCGA CCAGGCTGCT CTTGGGGCTC GAGCAAAAGC CCCCCAGTCA 
TTACCACACA GTGCCTCACT GCGGAGCGCA GGGAGCATGT CTGGGATCTC CAATAATATG 
CTTGTTGAAC CAGTGCAGAA TGGAAATAGG TCCAGACAGC AATCTACATC ATCTCGTGAA 
AGCCCTGTAC CCAAACTAAG AGCAATTCAC CGTCAGCAGA CACAGCAGGT GCAGTCACCT 
GTGGACTCCG CTACAATGTC TCCAGTAGAG CGGACTGCAG CCTGGGTTCT TAATAACGGT 
CAATATGAAG ATGAAGAAGA AACTGTAGAC CAAAACAAGG ATGAATCTAA ACCTGCAGAG 
AAGTATGAAC AAGAGATTGC AAAGCTGAAA GAACGATTGA AAATTTCAAG TCGGCGCCTG 
GAGGAATATG AACGGCGTCT TTTGGTGCAA GAGCAGCAAA TGCAGAAGCT CTTAATGGAG 
TACAAATCTC GACTGGAGGA TAGCGAGGAG AGGTTGCGCC GTCAGCAGGA AGAGAAGGAC 
AGCCAAATGA AAAGTGTCAT CAGCAGGTTA ATAGCTGTAG AAGAGGAGCT GAAAAAAGAC 
CATGCTGAGA TGCAGGCTGT CATTGATGCA AAGCAAAAAA TTATTGATGC ACAGGAGAAG 
AGGATCATAT CACTGGATGC AGCCAACACG CGGCTAATGA GTGCCCTCAC ACAGGTCAAG 
GAACGCTATA ACTCCCAGGT GCGCAATGGA ATCTCTCCGA CCAACCCTAC CAAGCTGTCA 
ATCACTGAAA ATGGAGAATT TAAGAACAGC AGTTGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rasal2 ( XM_031900971.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756831.1 Xenopus tropicalis RAS protein activator like 2 (rasal2), transcript variant X3, mRNA.

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CloneID	OXa57219
Clone ID Related Accession (Same CDS sequence)	XM_031900971.1
Accession Version	XM_031900971.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3711bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ras GTPase-activating protein nGAP isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCATGA TGGATTTATC CCCCGGCGGG GATTCGGTAA CATCCGGACA TGACTTCTTC 
CAGGACAGAG ATTCCCTCCC AGAGGATTTG GACTCTCCCG GGGGAGTGGG GGGGATTCTG 
GACAGGATCC TAATGGAGTC TGTGTGCCAG CAGCAGGGAT GGGTGCGAGT ATACGATGTG 
AAAGGCCCGC CTACACACAG GCTGTCGTGT GGTCAGTCGC CCTACACTGA AAGCACAACA 
TGGGAGCGCA AGTACTGTAT CCTAACAGAA AGCCAACTGG TGCTCCTTAA CCGTGACGAT 
GAGATGCCTG GGGAAACTGC ACAGGACCCA CCTCCAGAAT CCAGCAAAAG CAGAAGCCTG 
CGTAGGACTG TGAGTGTCCC GTCAGAAGGG CAGTTTCCAG ACTACCCCCC GGAGGGTGCT 
GCCAAGCTGG AGGTCCCAGC TGAGAGGTCA CCCCGAAGGA GGAGTATCTC AGGGACTAGC 
ACGTCAGACA AAACCAATGC TATGGATCCT GCAAATACCT CCCCCTTTAA AGTCTCAGGC 
TTCTTTAGTA AACGGTTAAA AGGCTCTATT AAGAGAACGA AGAGTCAATC AAAGCTGGAC 
CGGAATACAA GTTTTCGCCT GCCTTCTCTT CGTCCTGCTG AGAGTGACAG GTCACGTGGG 
TTGCCTAAAC TAAAAGAATC TCGTTCCCAT GAATCTTTGC TGAGCCCTGG TAGTGCAGTT 
GAGGCTCTGG ATTTGGGAAC TGAAGATAAA ATCCTAGTAA AACCGCTCCA CAGCAGCATA 
TTGGGACAAG ATTTCTGCTT TGAGGTCACC TATTCCAGTG GCAGTAAATG TTTCAGCTGC 
TCATCTGCAG CAGAGAGAGA TAAGTGGATG GAAAATCTTC GTCGAACTGT TCAACCAAAC 
AAGGATAACT GTCGCCGAGC AGAGAATGTA CTACGGCTGT GGATCATTGA AGCCAAGGAC 
CTGGCTCCCA AAAAGAAGTA CTTCTGTGAA CTTTGTCTGG ACGATACTCT CTTTGCTCGA 
ACCACAAGCA AGACGAAAGC GGACAATATT TTTTGGGGGG AATATTTTGA GTTTTATGGT 
TTACCACCCC TGCACAACAT CACAGTTCAT ATTTACAAGG ATGTTGAAAA GAAGAAAAAA 
AAAGATAAAA ACAATTATGT TGGTTTAGTG AATATTCCTA TGATTAGTGT AACTGGGAGG 
CAGTTTGTGG AGAAATGGTA CCCAGTTAGC ACCCCCACTG CAAACAAAGG CAAGACTGTT 
GGTCCTTCCA TACGTATAAA GTCCCGTTAC CAAACTATTA CCATTTTACC CATGGAGCAA 
TATAAGGAAT TTGCAGAATT TATCACAATT AATTACACTA TGCTATGCTC AGTCCTGGAA 
CCAGTCATTA GTGTCAGAAA TAAGGAAGAG ATGGCATGTG CTTTAGTGCA CATCCTGCAG 
AGTACTGGCA GAGCCAAGGA CTTTCTAACT GATCTGGTCA TGTCAGAGGT AGATCGTTGT 
GGGGAGCACG ATGTCTTGAT CTTTAGGGAA AATACACTTG CCACTAAAGC CATTGAGGAG 
TATCTAAAGC TGGTTGGACA GAAGTACCTT CATGATGCTC TTGGGGAGTT TATCAAGGCA 
CTGTATGAGT CGGATGAGAA CTGTGAGGTG GATCCCAGCA AATGTTCCTC AAGTGAGCTG 
TCCGACCACC AGAGCAACTT AAAAATGTGC TGCGAGCTGG CCTTCTGCAA GATAATCAAT 
TCATATTGTG TTTTTCCCAG AGAGCTGAAG GAAGTGTTTG CTTCTTGGAA GCAGCAATGC 
ATAAATCGGG GAAAGCAGGA TATCAGCGAA CGACTGATCA GCGCATCACT CTTCCTTCGC 
TTTCTTTGTC CTGCAATTAT GTCTCCCAGC CTCTTTGGGC TAATGCAGGA GTATCCTGAT 
GATCGCACCT CCAGAACTCT CACACTCATT GCCAAGGTCA TCCAGAATCT TGCCAACTTT 
GCCAAGTTTG GTAATAAAGA AGAATACATG GCCTTTATGA ATGACTTTTT GGAGCATGAG 
TGGGGTGGAA TGAAACGATT CCTACAAGAG ATCTCCAATC CAGACACCAT TTCAAACACC 
CCCGGATTTG AGGGTTACAT AGACCTTGGC CGAGAGCTCT CCGTACTGCA CAGTCTTTTG 
TGGGAGGTGG TGTCACAGCT AGATAAGGGT GAAAATTCCT TCCTACAGGC GACCGTTGAG 
AAATTGGGAC CCCTTCCACG CATTCTTGCT GACATCACCA AGTCACTGAC CAACCCTACT 
CCAATCCAAC AGCAGCTAAG ACGTTTCAGT GAACATAGCT CCACACCCAA TGTAAGTGGC 
AGCCTTTCAT CTGGACTTCA GAAGATTTTT GAGGATCCAG CAGACAGTGA TTTGCAGAGG 
TTCAGGTCTC CAACAATTGA AAACCCAGAA AGCTATTTCA GGGGGAAAAC GCTACTGCTT 
GTCCAGCAGG CATCCACACA GAGTATGTCC TTTTCCGACA AAGACGAAAG AGAGAATGTC 
TTGCCCAATG GGCGTAGTGT GTCTTTAATG GATCTGCAAG ACTCCAATGT TAGTCACACT 
GATCGTTCCT CAGTTATCCA AGAACCTCCC TTACGTTTAA CTGGCAGCCA GTTGTCTATT 
GCACATGTAG CTACCATTAA ACAGCTACGG GAAAACCATA GCATGCCTCA GAGTGCCCCA 
CAAGTTAGAA AGCCCTTACA TCCAGCATTG AGCCAGCAAA GCAGCCTGCA GCCTCTGTCC 
TTCCAAAATC CTGTGTATCA TCTGAACAAC CCACCACCGA CAATGCCCAA AGCTTCTGTG 
GACTCCAGCC TTGAAAATTT AAGCACTGCC AGCTCTAGAA GCCAGAGTAA TAGTGAAGAG 
TTCATTCTCA GTGGGCCCAG TAACAGTAGC ATGGAAGATT TCACAAAGAG GAGCACTCAG 
AGTGAGGACT TTACAAGAAG ACACACCATG CCTGAGAGAC CTATTCCCAT AGCACTGCCA 
AGGCAAAACA GCACTGGACA AGCCCAGATC CGTAAAACCG ACCAGGCTGC TCTTGGGGCT 
CGAGCAAAAG CCCCCCAGTC ATTACCACAC AGTGCCTCAC TGCGGAGCGC AGGGAGCATG 
TCTGGGATCT CCAATAATAT GCTTGTTGAA CCAGTGCAGA ATGGAAATAG GTCCAGACAG 
CAATCTACAT CATCTCGTGA AAGCCCTGTA CCCAAACTAA GAGCAATTCA CCGTCAGCAG 
ACACAGCAGG TGCAGTCACC TGTGGACTCC GCTACAATGT CTCCAGTAGA GCGGACTGCA 
GCCTGGGTTC TTAATAACGG TCAATATGAA GATGAAGAAG AAACTGTAGA CCAAAACAAG 
GATGAATCTA AACCTGCAGA GAAGTATGAA CAAGAGATTG CAAAGCTGAA AGAACGATTG 
AAAATTTCAA GTCGGCGCCT GGAGGAATAT GAACGGCGTC TTTTGGTGCA AGAGCAGCAA 
ATGCAGAAGC TCTTAATGGA GTACAAATCT CGACTGGAGG ATAGCGAGGA GAGGTTGCGC 
CGTCAGCAGG AAGAGAAGGA CAGCCAAATG AAAAGTGTCA TCAGCAGGTT AATAGCTGTA 
GAAGAGGAGC TGAAAAAAGA CCATGCTGAG ATGCAGGCTG TCATTGATGC AAAGCAAAAA 
ATTATTGATG CACAGGTCAT TAATGGGATT TTAACGCAAA CAGGCAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756831.1
CDS	659..4369
Translation	MLMMDLSPGGDSVTSGHDFFQDRDSLPEDLDSPGGVGGILDRILMESVCQQQGWVRVYDVKGPPTHRLSCGQSPYTESTTWERKYCILTESQLVLLNRDDEMPGETAQDPPPESSKSRSLRRTVSVPSEGQFPDYPPEGAAKLEVPAERSPRRRSISGTSTSDKTNAMDPANTSPFKVSGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRPAESDRSRGLPKLKESRSHESLLSPGSAVEALDLGTEDKILVKPLHSSILGQDFCFEVTYSSGSKCFSCSSAAERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEYFEFYGLPPLHNITVHIYKDVEKKKKKDKNNYVGLVNIPMISVTGRQFVEKWYPVSTPTANKGKTVGPSIRIKSRYQTITILPMEQYKEFAEFITINYTMLCSVLEPVISVRNKEEMACALVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTLATKAIEEYLKLVGQKYLHDALGEFIKALYESDENCEVDPSKCSSSELSDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCINRGKQDISERLISASLFLRFLCPAIMSPSLFGLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLQEISNPDTISNTPGFEGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVEKLGPLPRILADITKSLTNPTPIQQQLRRFSEHSSTPNVSGSLSSGLQKIFEDPADSDLQRFRSPTIENPESYFRGKTLLLVQQASTQSMSFSDKDERENVLPNGRSVSLMDLQDSNVSHTDRSSVIQEPPLRLTGSQLSIAHVATIKQLRENHSMPQSAPQVRKPLHPALSQQSSLQPLSFQNPVYHLNNPPPTMPKASVDSSLENLSTASSRSQSNSEEFILSGPSNSSMEDFTKRSTQSEDFTRRHTMPERPIPIALPRQNSTGQAQIRKTDQAALGARAKAPQSLPHSASLRSAGSMSGISNNMLVEPVQNGNRSRQQSTSSRESPVPKLRAIHRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEDEEETVDQNKDESKPAEKYEQEIAKLKERLKISSRRLEEYERRLLVQEQQMQKLLMEYKSRLEDSEERLRRQQEEKDSQMKSVISRLIAVEEELKKDHAEMQAVIDAKQKIIDAQVINGILTQTGK

Target ORF information:

RefSeq Version	XM_031900971.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis RAS protein activator like 2 (rasal2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031900971.1

ORF Insert Sequence:

ATGCTCATGA TGGATTTATC CCCCGGCGGG GATTCGGTAA CATCCGGACA TGACTTCTTC 
CAGGACAGAG ATTCCCTCCC AGAGGATTTG GACTCTCCCG GGGGAGTGGG GGGGATTCTG 
GACAGGATCC TAATGGAGTC TGTGTGCCAG CAGCAGGGAT GGGTGCGAGT ATACGATGTG 
AAAGGCCCGC CTACACACAG GCTGTCGTGT GGTCAGTCGC CCTACACTGA AAGCACAACA 
TGGGAGCGCA AGTACTGTAT CCTAACAGAA AGCCAACTGG TGCTCCTTAA CCGTGACGAT 
GAGATGCCTG GGGAAACTGC ACAGGACCCA CCTCCAGAAT CCAGCAAAAG CAGAAGCCTG 
CGTAGGACTG TGAGTGTCCC GTCAGAAGGG CAGTTTCCAG ACTACCCCCC GGAGGGTGCT 
GCCAAGCTGG AGGTCCCAGC TGAGAGGTCA CCCCGAAGGA GGAGTATCTC AGGGACTAGC 
ACGTCAGACA AAACCAATGC TATGGATCCT GCAAATACCT CCCCCTTTAA AGTCTCAGGC 
TTCTTTAGTA AACGGTTAAA AGGCTCTATT AAGAGAACGA AGAGTCAATC AAAGCTGGAC 
CGGAATACAA GTTTTCGCCT GCCTTCTCTT CGTCCTGCTG AGAGTGACAG GTCACGTGGG 
TTGCCTAAAC TAAAAGAATC TCGTTCCCAT GAATCTTTGC TGAGCCCTGG TAGTGCAGTT 
GAGGCTCTGG ATTTGGGAAC TGAAGATAAA ATCCTAGTAA AACCGCTCCA CAGCAGCATA 
TTGGGACAAG ATTTCTGCTT TGAGGTCACC TATTCCAGTG GCAGTAAATG TTTCAGCTGC 
TCATCTGCAG CAGAGAGAGA TAAGTGGATG GAAAATCTTC GTCGAACTGT TCAACCAAAC 
AAGGATAACT GTCGCCGAGC AGAGAATGTA CTACGGCTGT GGATCATTGA AGCCAAGGAC 
CTGGCTCCCA AAAAGAAGTA CTTCTGTGAA CTTTGTCTGG ACGATACTCT CTTTGCTCGA 
ACCACAAGCA AGACGAAAGC GGACAATATT TTTTGGGGGG AATATTTTGA GTTTTATGGT 
TTACCACCCC TGCACAACAT CACAGTTCAT ATTTACAAGG ATGTTGAAAA GAAGAAAAAA 
AAAGATAAAA ACAATTATGT TGGTTTAGTG AATATTCCTA TGATTAGTGT AACTGGGAGG 
CAGTTTGTGG AGAAATGGTA CCCAGTTAGC ACCCCCACTG CAAACAAAGG CAAGACTGTT 
GGTCCTTCCA TACGTATAAA GTCCCGTTAC CAAACTATTA CCATTTTACC CATGGAGCAA 
TATAAGGAAT TTGCAGAATT TATCACAATT AATTACACTA TGCTATGCTC AGTCCTGGAA 
CCAGTCATTA GTGTCAGAAA TAAGGAAGAG ATGGCATGTG CTTTAGTGCA CATCCTGCAG 
AGTACTGGCA GAGCCAAGGA CTTTCTAACT GATCTGGTCA TGTCAGAGGT AGATCGTTGT 
GGGGAGCACG ATGTCTTGAT CTTTAGGGAA AATACACTTG CCACTAAAGC CATTGAGGAG 
TATCTAAAGC TGGTTGGACA GAAGTACCTT CATGATGCTC TTGGGGAGTT TATCAAGGCA 
CTGTATGAGT CGGATGAGAA CTGTGAGGTG GATCCCAGCA AATGTTCCTC AAGTGAGCTG 
TCCGACCACC AGAGCAACTT AAAAATGTGC TGCGAGCTGG CCTTCTGCAA GATAATCAAT 
TCATATTGTG TTTTTCCCAG AGAGCTGAAG GAAGTGTTTG CTTCTTGGAA GCAGCAATGC 
ATAAATCGGG GAAAGCAGGA TATCAGCGAA CGACTGATCA GCGCATCACT CTTCCTTCGC 
TTTCTTTGTC CTGCAATTAT GTCTCCCAGC CTCTTTGGGC TAATGCAGGA GTATCCTGAT 
GATCGCACCT CCAGAACTCT CACACTCATT GCCAAGGTCA TCCAGAATCT TGCCAACTTT 
GCCAAGTTTG GTAATAAAGA AGAATACATG GCCTTTATGA ATGACTTTTT GGAGCATGAG 
TGGGGTGGAA TGAAACGATT CCTACAAGAG ATCTCCAATC CAGACACCAT TTCAAACACC 
CCCGGATTTG AGGGTTACAT AGACCTTGGC CGAGAGCTCT CCGTACTGCA CAGTCTTTTG 
TGGGAGGTGG TGTCACAGCT AGATAAGGGT GAAAATTCCT TCCTACAGGC GACCGTTGAG 
AAATTGGGAC CCCTTCCACG CATTCTTGCT GACATCACCA AGTCACTGAC CAACCCTACT 
CCAATCCAAC AGCAGCTAAG ACGTTTCAGT GAACATAGCT CCACACCCAA TGTAAGTGGC 
AGCCTTTCAT CTGGACTTCA GAAGATTTTT GAGGATCCAG CAGACAGTGA TTTGCAGAGG 
TTCAGGTCTC CAACAATTGA AAACCCAGAA AGCTATTTCA GGGGGAAAAC GCTACTGCTT 
GTCCAGCAGG CATCCACACA GAGTATGTCC TTTTCCGACA AAGACGAAAG AGAGAATGTC 
TTGCCCAATG GGCGTAGTGT GTCTTTAATG GATCTGCAAG ACTCCAATGT TAGTCACACT 
GATCGTTCCT CAGTTATCCA AGAACCTCCC TTACGTTTAA CTGGCAGCCA GTTGTCTATT 
GCACATGTAG CTACCATTAA ACAGCTACGG GAAAACCATA GCATGCCTCA GAGTGCCCCA 
CAAGTTAGAA AGCCCTTACA TCCAGCATTG AGCCAGCAAA GCAGCCTGCA GCCTCTGTCC 
TTCCAAAATC CTGTGTATCA TCTGAACAAC CCACCACCGA CAATGCCCAA AGCTTCTGTG 
GACTCCAGCC TTGAAAATTT AAGCACTGCC AGCTCTAGAA GCCAGAGTAA TAGTGAAGAG 
TTCATTCTCA GTGGGCCCAG TAACAGTAGC ATGGAAGATT TCACAAAGAG GAGCACTCAG 
AGTGAGGACT TTACAAGAAG ACACACCATG CCTGAGAGAC CTATTCCCAT AGCACTGCCA 
AGGCAAAACA GCACTGGACA AGCCCAGATC CGTAAAACCG ACCAGGCTGC TCTTGGGGCT 
CGAGCAAAAG CCCCCCAGTC ATTACCACAC AGTGCCTCAC TGCGGAGCGC AGGGAGCATG 
TCTGGGATCT CCAATAATAT GCTTGTTGAA CCAGTGCAGA ATGGAAATAG GTCCAGACAG 
CAATCTACAT CATCTCGTGA AAGCCCTGTA CCCAAACTAA GAGCAATTCA CCGTCAGCAG 
ACACAGCAGG TGCAGTCACC TGTGGACTCC GCTACAATGT CTCCAGTAGA GCGGACTGCA 
GCCTGGGTTC TTAATAACGG TCAATATGAA GATGAAGAAG AAACTGTAGA CCAAAACAAG 
GATGAATCTA AACCTGCAGA GAAGTATGAA CAAGAGATTG CAAAGCTGAA AGAACGATTG 
AAAATTTCAA GTCGGCGCCT GGAGGAATAT GAACGGCGTC TTTTGGTGCA AGAGCAGCAA 
ATGCAGAAGC TCTTAATGGA GTACAAATCT CGACTGGAGG ATAGCGAGGA GAGGTTGCGC 
CGTCAGCAGG AAGAGAAGGA CAGCCAAATG AAAAGTGTCA TCAGCAGGTT AATAGCTGTA 
GAAGAGGAGC TGAAAAAAGA CCATGCTGAG ATGCAGGCTG TCATTGATGC AAAGCAAAAA 
ATTATTGATG CACAGGTCAT TAATGGGATT TTAACGCAAA CAGGCAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rasal2 ( XM_031900972.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756832.1 Xenopus tropicalis RAS protein activator like 2 (rasal2), transcript variant X4, mRNA.

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CloneID	OXa57220
Clone ID Related Accession (Same CDS sequence)	XM_031900972.1
Accession Version	XM_031900972.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ras GTPase-activating protein nGAP isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGGGG AAACTGCACA GGACCCACCT CCAGAATCCA GCAAAAGCAG AAGCCTGCGT 
AGGACTGTGA GTGTCCCGTC AGAAGGGCAG TTTCCAGACT ACCCCCCGGA GGGTGCTGCC 
AAGCTGGAGG TCCCAGCTGA GAGGTCACCC CGAAGGAGGA GTATCTCAGG GACTAGCACG 
TCAGACAAAA CCAATGCTAT GGATCCTGCA AATACCTCCC CCTTTAAAGT CTCAGGCTTC 
TTTAGTAAAC GGTTAAAAGG CTCTATTAAG AGAACGAAGA GTCAATCAAA GCTGGACCGG 
AATACAAGTT TTCGCCTGCC TTCTCTTCGT CCTGCTGAGA GTGACAGGTC ACGTGGGTTG 
CCTAAACTAA AAGAATCTCG TTCCCATGAA TCTTTGCTGA GCCCTGGTAG TGCAGTTGAG 
GCTCTGGATT TGGGAACTGA AGATAAAATC CTAGTAAAAC CGCTCCACAG CAGCATATTG 
GGACAAGATT TCTGCTTTGA GGTCACCTAT TCCAGTGGCA GTAAATGTTT CAGCTGCTCA 
TCTGCAGCAG AGAGAGATAA GTGGATGGAA AATCTTCGTC GAACTGTTCA ACCAAACAAG 
GATAACTGTC GCCGAGCAGA GAATGTACTA CGGCTGTGGA TCATTGAAGC CAAGGACCTG 
GCTCCCAAAA AGAAGTACTT CTGTGAACTT TGTCTGGACG ATACTCTCTT TGCTCGAACC 
ACAAGCAAGA CGAAAGCGGA CAATATTTTT TGGGGGGAAT ATTTTGAGTT TTATGGTTTA 
CCACCCCTGC ACAACATCAC AGTTCATATT TACAAGGATG TTGAAAAGAA GAAAAAAAAA 
GATAAAAACA ATTATGTTGG TTTAGTGAAT ATTCCTATGA TTAGTGTAAC TGGGAGGCAG 
TTTGTGGAGA AATGGTACCC AGTTAGCACC CCCACTGCAA ACAAAGGCAA GACTGTTGGT 
CCTTCCATAC GTATAAAGTC CCGTTACCAA ACTATTACCA TTTTACCCAT GGAGCAATAT 
AAGGAATTTG CAGAATTTAT CACAATTAAT TACACTATGC TATGCTCAGT CCTGGAACCA 
GTCATTAGTG TCAGAAATAA GGAAGAGATG GCATGTGCTT TAGTGCACAT CCTGCAGAGT 
ACTGGCAGAG CCAAGGACTT TCTAACTGAT CTGGTCATGT CAGAGGTAGA TCGTTGTGGG 
GAGCACGATG TCTTGATCTT TAGGGAAAAT ACACTTGCCA CTAAAGCCAT TGAGGAGTAT 
CTAAAGCTGG TTGGACAGAA GTACCTTCAT GATGCTCTTG GGGAGTTTAT CAAGGCACTG 
TATGAGTCGG ATGAGAACTG TGAGGTGGAT CCCAGCAAAT GTTCCTCAAG TGAGCTGTCC 
GACCACCAGA GCAACTTAAA AATGTGCTGC GAGCTGGCCT TCTGCAAGAT AATCAATTCA 
TATTGTGTTT TTCCCAGAGA GCTGAAGGAA GTGTTTGCTT CTTGGAAGCA GCAATGCATA 
AATCGGGGAA AGCAGGATAT CAGCGAACGA CTGATCAGCG CATCACTCTT CCTTCGCTTT 
CTTTGTCCTG CAATTATGTC TCCCAGCCTC TTTGGGCTAA TGCAGGAGTA TCCTGATGAT 
CGCACCTCCA GAACTCTCAC ACTCATTGCC AAGGTCATCC AGAATCTTGC CAACTTTGCC 
AAGTTTGGTA ATAAAGAAGA ATACATGGCC TTTATGAATG ACTTTTTGGA GCATGAGTGG 
GGTGGAATGA AACGATTCCT ACAAGAGATC TCCAATCCAG ACACCATTTC AAACACCCCC 
GGATTTGAGG GTTACATAGA CCTTGGCCGA GAGCTCTCCG TACTGCACAG TCTTTTGTGG 
GAGGTGGTGT CACAGCTAGA TAAGGGTGAA AATTCCTTCC TACAGGCGAC CGTTGAGAAA 
TTGGGACCCC TTCCACGCAT TCTTGCTGAC ATCACCAAGT CACTGACCAA CCCTACTCCA 
ATCCAACAGC AGCTAAGACG TTTCAGTGAA CATAGCTCCA CACCCAATGT AAGTGGCAGC 
CTTTCATCTG GACTTCAGAA GATTTTTGAG GATCCAGCAG ACAGTGATTT GCAGAGGTTC 
AGGTCTCCAA CAATTGAAAA CCCAGAAAGC TATTTCAGGG GGAAAACGCT ACTGCTTGTC 
CAGCAGGCAT CCACACAGAG TATGTCCTTT TCCGACAAAG ACGAAAGAGA GAATGTCTTG 
CCCAATGGGC GTAGTGTGTC TTTAATGGAT CTGCAAGACT CCAATGTTAG TCACACTGAT 
CGTTCCTCAG TTATCCAAGA ACCTCCCTTA CGTTTAACTG GCAGCCAGTT GTCTATTGCA 
CATGTAGCTA CCATTAAACA GCTACGGGAA AACCATAGCA TGCCTCAGAG TGCCCCACAA 
GTTAGAAAGC CCTTACATCC AGCATTGAGC CAGCAAAGCA GCCTGCAGCC TCTGTCCTTC 
CAAAATCCTG TGTATCATCT GAACAACCCA CCACCGACAA TGCCCAAAGC TTCTGTGGAC 
TCCAGCCTTG AAAATTTAAG CACTGCCAGC TCTAGAAGCC AGAGTAATAG TGAAGAGTTC 
ATTCTCAGTG GGCCCAGTAA CAGTAGCATG GAAGATTTCA CAAAGAGGAG CACTCAGAGT 
GAGGACTTTA CAAGAAGACA CACCATGCCT GAGAGACCTA TTCCCATAGC ACTGCCAAGG 
CAAAACAGCA CTGGACAAGC CCAGATCCGT AAAACCGACC AGGCTGCTCT TGGGGCTCGA 
GCAAAAGCCC CCCAGTCATT ACCACACAGT GCCTCACTGC GGAGCGCAGG GAGCATGTCT 
GGGATCTCCA ATAATATGCT TGTTGAACCA GTGCAGAATG GAAATAGGTC CAGACAGCAA 
TCTACATCAT CTCGTGAAAG CCCTGTACCC AAACTAAGAG CAATTCACCG TCAGCAGACA 
CAGCAGGTGC AGTCACCTGT GGACTCCGCT ACAATGTCTC CAGTAGAGCG GACTGCAGCC 
TGGGTTCTTA ATAACGGTCA ATATGAAGAT GAAGAAGAAA CTGTAGACCA AAACAAGGAT 
GAATCTAAAC CTGCAGAGAA GTATGAACAA GAGATTGCAA AGCTGAAAGA ACGATTGAAA 
ATTTCAAGTC GGCGCCTGGA GGAATATGAA CGGCGTCTTT TGGTGCAAGA GCAGCAAATG 
CAGAAGCTCT TAATGGAGTA CAAATCTCGA CTGGAGGATA GCGAGGAGAG GTTGCGCCGT 
CAGCAGGAAG AGAAGGACAG CCAAATGAAA AGTGTCATCA GCAGGTTAAT AGCTGTAGAA 
GAGGAGCTGA AAAAAGACCA TGCTGAGATG CAGGCTGTCA TTGATGCAAA GCAAAAAATT 
ATTGATGCAC AGGAGAAGAG GATCATATCA CTGGATGCAG CCAACACGCG GCTAATGAGT 
GCCCTCACAC AGGTCAAGGA ACGCTATAAC TCCCAGGTGC GCAATGGAAT CTCTCCGACC 
AACCCTACCA AGCTGTCAAT CACTGAAAAT GGAGAATTTA AGAACAGCAG TTGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756832.1
CDS	207..3743
Translation	MPGETAQDPPPESSKSRSLRRTVSVPSEGQFPDYPPEGAAKLEVPAERSPRRRSISGTSTSDKTNAMDPANTSPFKVSGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRPAESDRSRGLPKLKESRSHESLLSPGSAVEALDLGTEDKILVKPLHSSILGQDFCFEVTYSSGSKCFSCSSAAERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEYFEFYGLPPLHNITVHIYKDVEKKKKKDKNNYVGLVNIPMISVTGRQFVEKWYPVSTPTANKGKTVGPSIRIKSRYQTITILPMEQYKEFAEFITINYTMLCSVLEPVISVRNKEEMACALVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTLATKAIEEYLKLVGQKYLHDALGEFIKALYESDENCEVDPSKCSSSELSDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCINRGKQDISERLISASLFLRFLCPAIMSPSLFGLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLQEISNPDTISNTPGFEGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVEKLGPLPRILADITKSLTNPTPIQQQLRRFSEHSSTPNVSGSLSSGLQKIFEDPADSDLQRFRSPTIENPESYFRGKTLLLVQQASTQSMSFSDKDERENVLPNGRSVSLMDLQDSNVSHTDRSSVIQEPPLRLTGSQLSIAHVATIKQLRENHSMPQSAPQVRKPLHPALSQQSSLQPLSFQNPVYHLNNPPPTMPKASVDSSLENLSTASSRSQSNSEEFILSGPSNSSMEDFTKRSTQSEDFTRRHTMPERPIPIALPRQNSTGQAQIRKTDQAALGARAKAPQSLPHSASLRSAGSMSGISNNMLVEPVQNGNRSRQQSTSSRESPVPKLRAIHRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEDEEETVDQNKDESKPAEKYEQEIAKLKERLKISSRRLEEYERRLLVQEQQMQKLLMEYKSRLEDSEERLRRQQEEKDSQMKSVISRLIAVEEELKKDHAEMQAVIDAKQKIIDAQEKRIISLDAANTRLMSALTQVKERYNSQVRNGISPTNPTKLSITENGEFKNSSC

Target ORF information:

RefSeq Version	XM_031900972.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis RAS protein activator like 2 (rasal2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031900972.1

ORF Insert Sequence:

ATGCCTGGGG AAACTGCACA GGACCCACCT CCAGAATCCA GCAAAAGCAG AAGCCTGCGT 
AGGACTGTGA GTGTCCCGTC AGAAGGGCAG TTTCCAGACT ACCCCCCGGA GGGTGCTGCC 
AAGCTGGAGG TCCCAGCTGA GAGGTCACCC CGAAGGAGGA GTATCTCAGG GACTAGCACG 
TCAGACAAAA CCAATGCTAT GGATCCTGCA AATACCTCCC CCTTTAAAGT CTCAGGCTTC 
TTTAGTAAAC GGTTAAAAGG CTCTATTAAG AGAACGAAGA GTCAATCAAA GCTGGACCGG 
AATACAAGTT TTCGCCTGCC TTCTCTTCGT CCTGCTGAGA GTGACAGGTC ACGTGGGTTG 
CCTAAACTAA AAGAATCTCG TTCCCATGAA TCTTTGCTGA GCCCTGGTAG TGCAGTTGAG 
GCTCTGGATT TGGGAACTGA AGATAAAATC CTAGTAAAAC CGCTCCACAG CAGCATATTG 
GGACAAGATT TCTGCTTTGA GGTCACCTAT TCCAGTGGCA GTAAATGTTT CAGCTGCTCA 
TCTGCAGCAG AGAGAGATAA GTGGATGGAA AATCTTCGTC GAACTGTTCA ACCAAACAAG 
GATAACTGTC GCCGAGCAGA GAATGTACTA CGGCTGTGGA TCATTGAAGC CAAGGACCTG 
GCTCCCAAAA AGAAGTACTT CTGTGAACTT TGTCTGGACG ATACTCTCTT TGCTCGAACC 
ACAAGCAAGA CGAAAGCGGA CAATATTTTT TGGGGGGAAT ATTTTGAGTT TTATGGTTTA 
CCACCCCTGC ACAACATCAC AGTTCATATT TACAAGGATG TTGAAAAGAA GAAAAAAAAA 
GATAAAAACA ATTATGTTGG TTTAGTGAAT ATTCCTATGA TTAGTGTAAC TGGGAGGCAG 
TTTGTGGAGA AATGGTACCC AGTTAGCACC CCCACTGCAA ACAAAGGCAA GACTGTTGGT 
CCTTCCATAC GTATAAAGTC CCGTTACCAA ACTATTACCA TTTTACCCAT GGAGCAATAT 
AAGGAATTTG CAGAATTTAT CACAATTAAT TACACTATGC TATGCTCAGT CCTGGAACCA 
GTCATTAGTG TCAGAAATAA GGAAGAGATG GCATGTGCTT TAGTGCACAT CCTGCAGAGT 
ACTGGCAGAG CCAAGGACTT TCTAACTGAT CTGGTCATGT CAGAGGTAGA TCGTTGTGGG 
GAGCACGATG TCTTGATCTT TAGGGAAAAT ACACTTGCCA CTAAAGCCAT TGAGGAGTAT 
CTAAAGCTGG TTGGACAGAA GTACCTTCAT GATGCTCTTG GGGAGTTTAT CAAGGCACTG 
TATGAGTCGG ATGAGAACTG TGAGGTGGAT CCCAGCAAAT GTTCCTCAAG TGAGCTGTCC 
GACCACCAGA GCAACTTAAA AATGTGCTGC GAGCTGGCCT TCTGCAAGAT AATCAATTCA 
TATTGTGTTT TTCCCAGAGA GCTGAAGGAA GTGTTTGCTT CTTGGAAGCA GCAATGCATA 
AATCGGGGAA AGCAGGATAT CAGCGAACGA CTGATCAGCG CATCACTCTT CCTTCGCTTT 
CTTTGTCCTG CAATTATGTC TCCCAGCCTC TTTGGGCTAA TGCAGGAGTA TCCTGATGAT 
CGCACCTCCA GAACTCTCAC ACTCATTGCC AAGGTCATCC AGAATCTTGC CAACTTTGCC 
AAGTTTGGTA ATAAAGAAGA ATACATGGCC TTTATGAATG ACTTTTTGGA GCATGAGTGG 
GGTGGAATGA AACGATTCCT ACAAGAGATC TCCAATCCAG ACACCATTTC AAACACCCCC 
GGATTTGAGG GTTACATAGA CCTTGGCCGA GAGCTCTCCG TACTGCACAG TCTTTTGTGG 
GAGGTGGTGT CACAGCTAGA TAAGGGTGAA AATTCCTTCC TACAGGCGAC CGTTGAGAAA 
TTGGGACCCC TTCCACGCAT TCTTGCTGAC ATCACCAAGT CACTGACCAA CCCTACTCCA 
ATCCAACAGC AGCTAAGACG TTTCAGTGAA CATAGCTCCA CACCCAATGT AAGTGGCAGC 
CTTTCATCTG GACTTCAGAA GATTTTTGAG GATCCAGCAG ACAGTGATTT GCAGAGGTTC 
AGGTCTCCAA CAATTGAAAA CCCAGAAAGC TATTTCAGGG GGAAAACGCT ACTGCTTGTC 
CAGCAGGCAT CCACACAGAG TATGTCCTTT TCCGACAAAG ACGAAAGAGA GAATGTCTTG 
CCCAATGGGC GTAGTGTGTC TTTAATGGAT CTGCAAGACT CCAATGTTAG TCACACTGAT 
CGTTCCTCAG TTATCCAAGA ACCTCCCTTA CGTTTAACTG GCAGCCAGTT GTCTATTGCA 
CATGTAGCTA CCATTAAACA GCTACGGGAA AACCATAGCA TGCCTCAGAG TGCCCCACAA 
GTTAGAAAGC CCTTACATCC AGCATTGAGC CAGCAAAGCA GCCTGCAGCC TCTGTCCTTC 
CAAAATCCTG TGTATCATCT GAACAACCCA CCACCGACAA TGCCCAAAGC TTCTGTGGAC 
TCCAGCCTTG AAAATTTAAG CACTGCCAGC TCTAGAAGCC AGAGTAATAG TGAAGAGTTC 
ATTCTCAGTG GGCCCAGTAA CAGTAGCATG GAAGATTTCA CAAAGAGGAG CACTCAGAGT 
GAGGACTTTA CAAGAAGACA CACCATGCCT GAGAGACCTA TTCCCATAGC ACTGCCAAGG 
CAAAACAGCA CTGGACAAGC CCAGATCCGT AAAACCGACC AGGCTGCTCT TGGGGCTCGA 
GCAAAAGCCC CCCAGTCATT ACCACACAGT GCCTCACTGC GGAGCGCAGG GAGCATGTCT 
GGGATCTCCA ATAATATGCT TGTTGAACCA GTGCAGAATG GAAATAGGTC CAGACAGCAA 
TCTACATCAT CTCGTGAAAG CCCTGTACCC AAACTAAGAG CAATTCACCG TCAGCAGACA 
CAGCAGGTGC AGTCACCTGT GGACTCCGCT ACAATGTCTC CAGTAGAGCG GACTGCAGCC 
TGGGTTCTTA ATAACGGTCA ATATGAAGAT GAAGAAGAAA CTGTAGACCA AAACAAGGAT 
GAATCTAAAC CTGCAGAGAA GTATGAACAA GAGATTGCAA AGCTGAAAGA ACGATTGAAA 
ATTTCAAGTC GGCGCCTGGA GGAATATGAA CGGCGTCTTT TGGTGCAAGA GCAGCAAATG 
CAGAAGCTCT TAATGGAGTA CAAATCTCGA CTGGAGGATA GCGAGGAGAG GTTGCGCCGT 
CAGCAGGAAG AGAAGGACAG CCAAATGAAA AGTGTCATCA GCAGGTTAAT AGCTGTAGAA 
GAGGAGCTGA AAAAAGACCA TGCTGAGATG CAGGCTGTCA TTGATGCAAA GCAAAAAATT 
ATTGATGCAC AGGAGAAGAG GATCATATCA CTGGATGCAG CCAACACGCG GCTAATGAGT 
GCCCTCACAC AGGTCAAGGA ACGCTATAAC TCCCAGGTGC GCAATGGAAT CTCTCCGACC 
AACCCTACCA AGCTGTCAAT CACTGAAAAT GGAGAATTTA AGAACAGCAG TTGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rasal2 ( XM_031900974.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756834.1 Xenopus tropicalis RAS protein activator like 2 (rasal2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXa57221
Clone ID Related Accession (Same CDS sequence)	XM_031900974.1
Accession Version	XM_031900974.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3489bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ras GTPase-activating protein nGAP isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTTTC TGTCGCCAGG GGATGGAGAG TGGTTGGATA GCAGCACAGA GGAAGAGAAG 
AGTCTCCTGA CATTTCTCGA GGTCCCAGCT GAGAGGTCAC CCCGAAGGAG GAGTATCTCA 
GGGACTAGCA CGTCAGACAA AACCAATGCT ATGGATCCTG CAAATACCTC CCCCTTTAAA 
GTCTCAGGCT TCTTTAGTAA ACGGTTAAAA GGCTCTATTA AGAGAACGAA GAGTCAATCA 
AAGCTGGACC GGAATACAAG TTTTCGCCTG CCTTCTCTTC GTCCTGCTGA GAGTGACAGG 
TCACGTGGGT TGCCTAAACT AAAAGAATCT CGTTCCCATG AATCTTTGCT GAGCCCTGGT 
AGTGCAGTTG AGGCTCTGGA TTTGGGAACT GAAGATAAAA TCCTAGTAAA ACCGCTCCAC 
AGCAGCATAT TGGGACAAGA TTTCTGCTTT GAGGTCACCT ATTCCAGTGG CAGTAAATGT 
TTCAGCTGCT CATCTGCAGC AGAGAGAGAT AAGTGGATGG AAAATCTTCG TCGAACTGTT 
CAACCAAACA AGGATAACTG TCGCCGAGCA GAGAATGTAC TACGGCTGTG GATCATTGAA 
GCCAAGGACC TGGCTCCCAA AAAGAAGTAC TTCTGTGAAC TTTGTCTGGA CGATACTCTC 
TTTGCTCGAA CCACAAGCAA GACGAAAGCG GACAATATTT TTTGGGGGGA ATATTTTGAG 
TTTTATGGTT TACCACCCCT GCACAACATC ACAGTTCATA TTTACAAGGA TGTTGAAAAG 
AAGAAAAAAA AAGATAAAAA CAATTATGTT GGTTTAGTGA ATATTCCTAT GATTAGTGTA 
ACTGGGAGGC AGTTTGTGGA GAAATGGTAC CCAGTTAGCA CCCCCACTGC AAACAAAGGC 
AAGACTGTTG GTCCTTCCAT ACGTATAAAG TCCCGTTACC AAACTATTAC CATTTTACCC 
ATGGAGCAAT ATAAGGAATT TGCAGAATTT ATCACAATTA ATTACACTAT GCTATGCTCA 
GTCCTGGAAC CAGTCATTAG TGTCAGAAAT AAGGAAGAGA TGGCATGTGC TTTAGTGCAC 
ATCCTGCAGA GTACTGGCAG AGCCAAGGAC TTTCTAACTG ATCTGGTCAT GTCAGAGGTA 
GATCGTTGTG GGGAGCACGA TGTCTTGATC TTTAGGGAAA ATACACTTGC CACTAAAGCC 
ATTGAGGAGT ATCTAAAGCT GGTTGGACAG AAGTACCTTC ATGATGCTCT TGGGGAGTTT 
ATCAAGGCAC TGTATGAGTC GGATGAGAAC TGTGAGGTGG ATCCCAGCAA ATGTTCCTCA 
AGTGAGCTGT CCGACCACCA GAGCAACTTA AAAATGTGCT GCGAGCTGGC CTTCTGCAAG 
ATAATCAATT CATATTGTGT TTTTCCCAGA GAGCTGAAGG AAGTGTTTGC TTCTTGGAAG 
CAGCAATGCA TAAATCGGGG AAAGCAGGAT ATCAGCGAAC GACTGATCAG CGCATCACTC 
TTCCTTCGCT TTCTTTGTCC TGCAATTATG TCTCCCAGCC TCTTTGGGCT AATGCAGGAG 
TATCCTGATG ATCGCACCTC CAGAACTCTC ACACTCATTG CCAAGGTCAT CCAGAATCTT 
GCCAACTTTG CCAAGTTTGG TAATAAAGAA GAATACATGG CCTTTATGAA TGACTTTTTG 
GAGCATGAGT GGGGTGGAAT GAAACGATTC CTACAAGAGA TCTCCAATCC AGACACCATT 
TCAAACACCC CCGGATTTGA GGGTTACATA GACCTTGGCC GAGAGCTCTC CGTACTGCAC 
AGTCTTTTGT GGGAGGTGGT GTCACAGCTA GATAAGGGTG AAAATTCCTT CCTACAGGCG 
ACCGTTGAGA AATTGGGACC CCTTCCACGC ATTCTTGCTG ACATCACCAA GTCACTGACC 
AACCCTACTC CAATCCAACA GCAGCTAAGA CGTTTCAGTG AACATAGCTC CACACCCAAT 
GTAAGTGGCA GCCTTTCATC TGGACTTCAG AAGATTTTTG AGGATCCAGC AGACAGTGAT 
TTGCAGAGGT TCAGGTCTCC AACAATTGAA AACCCAGAAA GCTATTTCAG GGGGAAAACG 
CTACTGCTTG TCCAGCAGGC ATCCACACAG AGTATGTCCT TTTCCGACAA AGACGAAAGA 
GAGAATGTCT TGCCCAATGG GCGTAGTGTG TCTTTAATGG ATCTGCAAGA CTCCAATGTT 
AGTCACACTG ATCGTTCCTC AGTTATCCAA GAACCTCCCT TACGTTTAAC TGGCAGCCAG 
TTGTCTATTG CACATGTAGC TACCATTAAA CAGCTACGGG AAAACCATAG CATGCCTCAG 
AGTGCCCCAC AAGTTAGAAA GCCCTTACAT CCAGCATTGA GCCAGCAAAG CAGCCTGCAG 
CCTCTGTCCT TCCAAAATCC TGTGTATCAT CTGAACAACC CACCACCGAC AATGCCCAAA 
GCTTCTGTGG ACTCCAGCCT TGAAAATTTA AGCACTGCCA GCTCTAGAAG CCAGAGTAAT 
AGTGAAGAGT TCATTCTCAG TGGGCCCAGT AACAGTAGCA TGGAAGATTT CACAAAGAGG 
AGCACTCAGA GTGAGGACTT TACAAGAAGA CACACCATGC CTGAGAGACC TATTCCCATA 
GCACTGCCAA GGCAAAACAG CACTGGACAA GCCCAGATCC GTAAAACCGA CCAGGCTGCT 
CTTGGGGCTC GAGCAAAAGC CCCCCAGTCA TTACCACACA GTGCCTCACT GCGGAGCGCA 
GGGAGCATGT CTGGGATCTC CAATAATATG CTTGTTGAAC CAGTGCAGAA TGGAAATAGG 
TCCAGACAGC AATCTACATC ATCTCGTGAA AGCCCTGTAC CCAAACTAAG AGCAATTCAC 
CGTCAGCAGA CACAGCAGGT GCAGTCACCT GTGGACTCCG CTACAATGTC TCCAGTAGAG 
CGGACTGCAG CCTGGGTTCT TAATAACGGT CAATATGAAG ATGAAGAAGA AACTGTAGAC 
CAAAACAAGG ATGAATCTAA ACCTGCAGAG AAGTATGAAC AAGAGATTGC AAAGCTGAAA 
GAACGATTGA AAATTTCAAG TCGGCGCCTG GAGGAATATG AACGGCGTCT TTTGGTGCAA 
GAGCAGCAAA TGCAGAAGCT CTTAATGGAG TACAAATCTC GACTGGAGGA TAGCGAGGAG 
AGGTTGCGCC GTCAGCAGGA AGAGAAGGAC AGCCAAATGA AAAGTGTCAT CAGCAGGTTA 
ATAGCTGTAG AAGAGGAGCT GAAAAAAGAC CATGCTGAGA TGCAGGCTGT CATTGATGCA 
AAGCAAAAAA TTATTGATGC ACAGGAGAAG AGGATCATAT CACTGGATGC AGCCAACACG 
CGGCTAATGA GTGCCCTCAC ACAGGTCAAG GAACGCTATA ACTCCCAGGT GCGCAATGGA 
ATCTCTCCGA CCAACCCTAC CAAGCTGTCA ATCACTGAAA ATGGAGAATT TAAGAACAGC 
AGTTGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756834.1
CDS	16..3504
Translation	MSFLSPGDGEWLDSSTEEEKSLLTFLEVPAERSPRRRSISGTSTSDKTNAMDPANTSPFKVSGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRPAESDRSRGLPKLKESRSHESLLSPGSAVEALDLGTEDKILVKPLHSSILGQDFCFEVTYSSGSKCFSCSSAAERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEYFEFYGLPPLHNITVHIYKDVEKKKKKDKNNYVGLVNIPMISVTGRQFVEKWYPVSTPTANKGKTVGPSIRIKSRYQTITILPMEQYKEFAEFITINYTMLCSVLEPVISVRNKEEMACALVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTLATKAIEEYLKLVGQKYLHDALGEFIKALYESDENCEVDPSKCSSSELSDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCINRGKQDISERLISASLFLRFLCPAIMSPSLFGLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLQEISNPDTISNTPGFEGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVEKLGPLPRILADITKSLTNPTPIQQQLRRFSEHSSTPNVSGSLSSGLQKIFEDPADSDLQRFRSPTIENPESYFRGKTLLLVQQASTQSMSFSDKDERENVLPNGRSVSLMDLQDSNVSHTDRSSVIQEPPLRLTGSQLSIAHVATIKQLRENHSMPQSAPQVRKPLHPALSQQSSLQPLSFQNPVYHLNNPPPTMPKASVDSSLENLSTASSRSQSNSEEFILSGPSNSSMEDFTKRSTQSEDFTRRHTMPERPIPIALPRQNSTGQAQIRKTDQAALGARAKAPQSLPHSASLRSAGSMSGISNNMLVEPVQNGNRSRQQSTSSRESPVPKLRAIHRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEDEEETVDQNKDESKPAEKYEQEIAKLKERLKISSRRLEEYERRLLVQEQQMQKLLMEYKSRLEDSEERLRRQQEEKDSQMKSVISRLIAVEEELKKDHAEMQAVIDAKQKIIDAQEKRIISLDAANTRLMSALTQVKERYNSQVRNGISPTNPTKLSITENGEFKNSSC

Target ORF information:

RefSeq Version	XM_031900974.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis RAS protein activator like 2 (rasal2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031900974.1

ORF Insert Sequence:

ATGAGTTTTC TGTCGCCAGG GGATGGAGAG TGGTTGGATA GCAGCACAGA GGAAGAGAAG 
AGTCTCCTGA CATTTCTCGA GGTCCCAGCT GAGAGGTCAC CCCGAAGGAG GAGTATCTCA 
GGGACTAGCA CGTCAGACAA AACCAATGCT ATGGATCCTG CAAATACCTC CCCCTTTAAA 
GTCTCAGGCT TCTTTAGTAA ACGGTTAAAA GGCTCTATTA AGAGAACGAA GAGTCAATCA 
AAGCTGGACC GGAATACAAG TTTTCGCCTG CCTTCTCTTC GTCCTGCTGA GAGTGACAGG 
TCACGTGGGT TGCCTAAACT AAAAGAATCT CGTTCCCATG AATCTTTGCT GAGCCCTGGT 
AGTGCAGTTG AGGCTCTGGA TTTGGGAACT GAAGATAAAA TCCTAGTAAA ACCGCTCCAC 
AGCAGCATAT TGGGACAAGA TTTCTGCTTT GAGGTCACCT ATTCCAGTGG CAGTAAATGT 
TTCAGCTGCT CATCTGCAGC AGAGAGAGAT AAGTGGATGG AAAATCTTCG TCGAACTGTT 
CAACCAAACA AGGATAACTG TCGCCGAGCA GAGAATGTAC TACGGCTGTG GATCATTGAA 
GCCAAGGACC TGGCTCCCAA AAAGAAGTAC TTCTGTGAAC TTTGTCTGGA CGATACTCTC 
TTTGCTCGAA CCACAAGCAA GACGAAAGCG GACAATATTT TTTGGGGGGA ATATTTTGAG 
TTTTATGGTT TACCACCCCT GCACAACATC ACAGTTCATA TTTACAAGGA TGTTGAAAAG 
AAGAAAAAAA AAGATAAAAA CAATTATGTT GGTTTAGTGA ATATTCCTAT GATTAGTGTA 
ACTGGGAGGC AGTTTGTGGA GAAATGGTAC CCAGTTAGCA CCCCCACTGC AAACAAAGGC 
AAGACTGTTG GTCCTTCCAT ACGTATAAAG TCCCGTTACC AAACTATTAC CATTTTACCC 
ATGGAGCAAT ATAAGGAATT TGCAGAATTT ATCACAATTA ATTACACTAT GCTATGCTCA 
GTCCTGGAAC CAGTCATTAG TGTCAGAAAT AAGGAAGAGA TGGCATGTGC TTTAGTGCAC 
ATCCTGCAGA GTACTGGCAG AGCCAAGGAC TTTCTAACTG ATCTGGTCAT GTCAGAGGTA 
GATCGTTGTG GGGAGCACGA TGTCTTGATC TTTAGGGAAA ATACACTTGC CACTAAAGCC 
ATTGAGGAGT ATCTAAAGCT GGTTGGACAG AAGTACCTTC ATGATGCTCT TGGGGAGTTT 
ATCAAGGCAC TGTATGAGTC GGATGAGAAC TGTGAGGTGG ATCCCAGCAA ATGTTCCTCA 
AGTGAGCTGT CCGACCACCA GAGCAACTTA AAAATGTGCT GCGAGCTGGC CTTCTGCAAG 
ATAATCAATT CATATTGTGT TTTTCCCAGA GAGCTGAAGG AAGTGTTTGC TTCTTGGAAG 
CAGCAATGCA TAAATCGGGG AAAGCAGGAT ATCAGCGAAC GACTGATCAG CGCATCACTC 
TTCCTTCGCT TTCTTTGTCC TGCAATTATG TCTCCCAGCC TCTTTGGGCT AATGCAGGAG 
TATCCTGATG ATCGCACCTC CAGAACTCTC ACACTCATTG CCAAGGTCAT CCAGAATCTT 
GCCAACTTTG CCAAGTTTGG TAATAAAGAA GAATACATGG CCTTTATGAA TGACTTTTTG 
GAGCATGAGT GGGGTGGAAT GAAACGATTC CTACAAGAGA TCTCCAATCC AGACACCATT 
TCAAACACCC CCGGATTTGA GGGTTACATA GACCTTGGCC GAGAGCTCTC CGTACTGCAC 
AGTCTTTTGT GGGAGGTGGT GTCACAGCTA GATAAGGGTG AAAATTCCTT CCTACAGGCG 
ACCGTTGAGA AATTGGGACC CCTTCCACGC ATTCTTGCTG ACATCACCAA GTCACTGACC 
AACCCTACTC CAATCCAACA GCAGCTAAGA CGTTTCAGTG AACATAGCTC CACACCCAAT 
GTAAGTGGCA GCCTTTCATC TGGACTTCAG AAGATTTTTG AGGATCCAGC AGACAGTGAT 
TTGCAGAGGT TCAGGTCTCC AACAATTGAA AACCCAGAAA GCTATTTCAG GGGGAAAACG 
CTACTGCTTG TCCAGCAGGC ATCCACACAG AGTATGTCCT TTTCCGACAA AGACGAAAGA 
GAGAATGTCT TGCCCAATGG GCGTAGTGTG TCTTTAATGG ATCTGCAAGA CTCCAATGTT 
AGTCACACTG ATCGTTCCTC AGTTATCCAA GAACCTCCCT TACGTTTAAC TGGCAGCCAG 
TTGTCTATTG CACATGTAGC TACCATTAAA CAGCTACGGG AAAACCATAG CATGCCTCAG 
AGTGCCCCAC AAGTTAGAAA GCCCTTACAT CCAGCATTGA GCCAGCAAAG CAGCCTGCAG 
CCTCTGTCCT TCCAAAATCC TGTGTATCAT CTGAACAACC CACCACCGAC AATGCCCAAA 
GCTTCTGTGG ACTCCAGCCT TGAAAATTTA AGCACTGCCA GCTCTAGAAG CCAGAGTAAT 
AGTGAAGAGT TCATTCTCAG TGGGCCCAGT AACAGTAGCA TGGAAGATTT CACAAAGAGG 
AGCACTCAGA GTGAGGACTT TACAAGAAGA CACACCATGC CTGAGAGACC TATTCCCATA 
GCACTGCCAA GGCAAAACAG CACTGGACAA GCCCAGATCC GTAAAACCGA CCAGGCTGCT 
CTTGGGGCTC GAGCAAAAGC CCCCCAGTCA TTACCACACA GTGCCTCACT GCGGAGCGCA 
GGGAGCATGT CTGGGATCTC CAATAATATG CTTGTTGAAC CAGTGCAGAA TGGAAATAGG 
TCCAGACAGC AATCTACATC ATCTCGTGAA AGCCCTGTAC CCAAACTAAG AGCAATTCAC 
CGTCAGCAGA CACAGCAGGT GCAGTCACCT GTGGACTCCG CTACAATGTC TCCAGTAGAG 
CGGACTGCAG CCTGGGTTCT TAATAACGGT CAATATGAAG ATGAAGAAGA AACTGTAGAC 
CAAAACAAGG ATGAATCTAA ACCTGCAGAG AAGTATGAAC AAGAGATTGC AAAGCTGAAA 
GAACGATTGA AAATTTCAAG TCGGCGCCTG GAGGAATATG AACGGCGTCT TTTGGTGCAA 
GAGCAGCAAA TGCAGAAGCT CTTAATGGAG TACAAATCTC GACTGGAGGA TAGCGAGGAG 
AGGTTGCGCC GTCAGCAGGA AGAGAAGGAC AGCCAAATGA AAAGTGTCAT CAGCAGGTTA 
ATAGCTGTAG AAGAGGAGCT GAAAAAAGAC CATGCTGAGA TGCAGGCTGT CATTGATGCA 
AAGCAAAAAA TTATTGATGC ACAGGAGAAG AGGATCATAT CACTGGATGC AGCCAACACG 
CGGCTAATGA GTGCCCTCAC ACAGGTCAAG GAACGCTATA ACTCCCAGGT GCGCAATGGA 
ATCTCTCCGA CCAACCCTAC CAAGCTGTCA ATCACTGAAA ATGGAGAATT TAAGAACAGC 
AGTTGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rasal2 ( XM_004914031.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004914088.1 Xenopus tropicalis RAS protein activator like 2 (rasal2), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OXa57222
Clone ID Related Accession (Same CDS sequence)	XM_004914031.4
Accession Version	XM_004914031.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3432bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ras GTPase-activating protein nGAP isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004914031.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTAGCA CATGCGATAA AGAGGTCCCA GCTGAGAGGT CACCCCGAAG GAGGAGTATC 
TCAGGGACTA GCACGTCAGA CAAAACCAAT GCTATGGATC CTGCAAATAC CTCCCCCTTT 
AAAGTCTCAG GCTTCTTTAG TAAACGGTTA AAAGGCTCTA TTAAGAGAAC GAAGAGTCAA 
TCAAAGCTGG ACCGGAATAC AAGTTTTCGC CTGCCTTCTC TTCGTCCTGC TGAGAGTGAC 
AGGTCACGTG GGTTGCCTAA ACTAAAAGAA TCTCGTTCCC ATGAATCTTT GCTGAGCCCT 
GGTAGTGCAG TTGAGGCTCT GGATTTGGGA ACTGAAGATA AAATCCTAGT AAAACCGCTC 
CACAGCAGCA TATTGGGACA AGATTTCTGC TTTGAGGTCA CCTATTCCAG TGGCAGTAAA 
TGTTTCAGCT GCTCATCTGC AGCAGAGAGA GATAAGTGGA TGGAAAATCT TCGTCGAACT 
GTTCAACCAA ACAAGGATAA CTGTCGCCGA GCAGAGAATG TACTACGGCT GTGGATCATT 
GAAGCCAAGG ACCTGGCTCC CAAAAAGAAG TACTTCTGTG AACTTTGTCT GGACGATACT 
CTCTTTGCTC GAACCACAAG CAAGACGAAA GCGGACAATA TTTTTTGGGG GGAATATTTT 
GAGTTTTATG GTTTACCACC CCTGCACAAC ATCACAGTTC ATATTTACAA GGATGTTGAA 
AAGAAGAAAA AAAAAGATAA AAACAATTAT GTTGGTTTAG TGAATATTCC TATGATTAGT 
GTAACTGGGA GGCAGTTTGT GGAGAAATGG TACCCAGTTA GCACCCCCAC TGCAAACAAA 
GGCAAGACTG TTGGTCCTTC CATACGTATA AAGTCCCGTT ACCAAACTAT TACCATTTTA 
CCCATGGAGC AATATAAGGA ATTTGCAGAA TTTATCACAA TTAATTACAC TATGCTATGC 
TCAGTCCTGG AACCAGTCAT TAGTGTCAGA AATAAGGAAG AGATGGCATG TGCTTTAGTG 
CACATCCTGC AGAGTACTGG CAGAGCCAAG GACTTTCTAA CTGATCTGGT CATGTCAGAG 
GTAGATCGTT GTGGGGAGCA CGATGTCTTG ATCTTTAGGG AAAATACACT TGCCACTAAA 
GCCATTGAGG AGTATCTAAA GCTGGTTGGA CAGAAGTACC TTCATGATGC TCTTGGGGAG 
TTTATCAAGG CACTGTATGA GTCGGATGAG AACTGTGAGG TGGATCCCAG CAAATGTTCC 
TCAAGTGAGC TGTCCGACCA CCAGAGCAAC TTAAAAATGT GCTGCGAGCT GGCCTTCTGC 
AAGATAATCA ATTCATATTG TGTTTTTCCC AGAGAGCTGA AGGAAGTGTT TGCTTCTTGG 
AAGCAGCAAT GCATAAATCG GGGAAAGCAG GATATCAGCG AACGACTGAT CAGCGCATCA 
CTCTTCCTTC GCTTTCTTTG TCCTGCAATT ATGTCTCCCA GCCTCTTTGG GCTAATGCAG 
GAGTATCCTG ATGATCGCAC CTCCAGAACT CTCACACTCA TTGCCAAGGT CATCCAGAAT 
CTTGCCAACT TTGCCAAGTT TGGTAATAAA GAAGAATACA TGGCCTTTAT GAATGACTTT 
TTGGAGCATG AGTGGGGTGG AATGAAACGA TTCCTACAAG AGATCTCCAA TCCAGACACC 
ATTTCAAACA CCCCCGGATT TGAGGGTTAC ATAGACCTTG GCCGAGAGCT CTCCGTACTG 
CACAGTCTTT TGTGGGAGGT GGTGTCACAG CTAGATAAGG GTGAAAATTC CTTCCTACAG 
GCGACCGTTG AGAAATTGGG ACCCCTTCCA CGCATTCTTG CTGACATCAC CAAGTCACTG 
ACCAACCCTA CTCCAATCCA ACAGCAGCTA AGACGTTTCA GTGAACATAG CTCCACACCC 
AATGTAAGTG GCAGCCTTTC ATCTGGACTT CAGAAGATTT TTGAGGATCC AGCAGACAGT 
GATTTGCAGA GGTTCAGGTC TCCAACAATT GAAAACCCAG AAAGCTATTT CAGGGGGAAA 
ACGCTACTGC TTGTCCAGCA GGCATCCACA CAGAGTATGT CCTTTTCCGA CAAAGACGAA 
AGAGAGAATG TCTTGCCCAA TGGGCGTAGT GTGTCTTTAA TGGATCTGCA AGACTCCAAT 
GTTAGTCACA CTGATCGTTC CTCAGTTATC CAAGAACCTC CCTTACGTTT AACTGGCAGC 
CAGTTGTCTA TTGCACATGT AGCTACCATT AAACAGCTAC GGGAAAACCA TAGCATGCCT 
CAGAGTGCCC CACAAGTTAG AAAGCCCTTA CATCCAGCAT TGAGCCAGCA AAGCAGCCTG 
CAGCCTCTGT CCTTCCAAAA TCCTGTGTAT CATCTGAACA ACCCACCACC GACAATGCCC 
AAAGCTTCTG TGGACTCCAG CCTTGAAAAT TTAAGCACTG CCAGCTCTAG AAGCCAGAGT 
AATAGTGAAG AGTTCATTCT CAGTGGGCCC AGTAACAGTA GCATGGAAGA TTTCACAAAG 
AGGAGCACTC AGAGTGAGGA CTTTACAAGA AGACACACCA TGCCTGAGAG ACCTATTCCC 
ATAGCACTGC CAAGGCAAAA CAGCACTGGA CAAGCCCAGA TCCGTAAAAC CGACCAGGCT 
GCTCTTGGGG CTCGAGCAAA AGCCCCCCAG TCATTACCAC ACAGTGCCTC ACTGCGGAGC 
GCAGGGAGCA TGTCTGGGAT CTCCAATAAT ATGCTTGTTG AACCAGTGCA GAATGGAAAT 
AGGTCCAGAC AGCAATCTAC ATCATCTCGT GAAAGCCCTG TACCCAAACT AAGAGCAATT 
CACCGTCAGC AGACACAGCA GGTGCAGTCA CCTGTGGACT CCGCTACAAT GTCTCCAGTA 
GAGCGGACTG CAGCCTGGGT TCTTAATAAC GGTCAATATG AAGATGAAGA AGAAACTGTA 
GACCAAAACA AGGATGAATC TAAACCTGCA GAGAAGTATG AACAAGAGAT TGCAAAGCTG 
AAAGAACGAT TGAAAATTTC AAGTCGGCGC CTGGAGGAAT ATGAACGGCG TCTTTTGGTG 
CAAGAGCAGC AAATGCAGAA GCTCTTAATG GAGTACAAAT CTCGACTGGA GGATAGCGAG 
GAGAGGTTGC GCCGTCAGCA GGAAGAGAAG GACAGCCAAA TGAAAAGTGT CATCAGCAGG 
TTAATAGCTG TAGAAGAGGA GCTGAAAAAA GACCATGCTG AGATGCAGGC TGTCATTGAT 
GCAAAGCAAA AAATTATTGA TGCACAGGAG AAGAGGATCA TATCACTGGA TGCAGCCAAC 
ACGCGGCTAA TGAGTGCCCT CACACAGGTC AAGGAACGCT ATAACTCCCA GGTGCGCAAT 
GGAATCTCTC CGACCAACCC TACCAAGCTG TCAATCACTG AAAATGGAGA ATTTAAGAAC 
AGCAGTTGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004914088.1
CDS	85..3516
Translation	MGSTCDKEVPAERSPRRRSISGTSTSDKTNAMDPANTSPFKVSGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRPAESDRSRGLPKLKESRSHESLLSPGSAVEALDLGTEDKILVKPLHSSILGQDFCFEVTYSSGSKCFSCSSAAERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEYFEFYGLPPLHNITVHIYKDVEKKKKKDKNNYVGLVNIPMISVTGRQFVEKWYPVSTPTANKGKTVGPSIRIKSRYQTITILPMEQYKEFAEFITINYTMLCSVLEPVISVRNKEEMACALVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTLATKAIEEYLKLVGQKYLHDALGEFIKALYESDENCEVDPSKCSSSELSDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCINRGKQDISERLISASLFLRFLCPAIMSPSLFGLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLQEISNPDTISNTPGFEGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVEKLGPLPRILADITKSLTNPTPIQQQLRRFSEHSSTPNVSGSLSSGLQKIFEDPADSDLQRFRSPTIENPESYFRGKTLLLVQQASTQSMSFSDKDERENVLPNGRSVSLMDLQDSNVSHTDRSSVIQEPPLRLTGSQLSIAHVATIKQLRENHSMPQSAPQVRKPLHPALSQQSSLQPLSFQNPVYHLNNPPPTMPKASVDSSLENLSTASSRSQSNSEEFILSGPSNSSMEDFTKRSTQSEDFTRRHTMPERPIPIALPRQNSTGQAQIRKTDQAALGARAKAPQSLPHSASLRSAGSMSGISNNMLVEPVQNGNRSRQQSTSSRESPVPKLRAIHRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEDEEETVDQNKDESKPAEKYEQEIAKLKERLKISSRRLEEYERRLLVQEQQMQKLLMEYKSRLEDSEERLRRQQEEKDSQMKSVISRLIAVEEELKKDHAEMQAVIDAKQKIIDAQEKRIISLDAANTRLMSALTQVKERYNSQVRNGISPTNPTKLSITENGEFKNSSC

Target ORF information:

RefSeq Version	XM_004914031.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis RAS protein activator like 2 (rasal2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004914031.4

ORF Insert Sequence:

ATGGGTAGCA CATGCGATAA AGAGGTCCCA GCTGAGAGGT CACCCCGAAG GAGGAGTATC 
TCAGGGACTA GCACGTCAGA CAAAACCAAT GCTATGGATC CTGCAAATAC CTCCCCCTTT 
AAAGTCTCAG GCTTCTTTAG TAAACGGTTA AAAGGCTCTA TTAAGAGAAC GAAGAGTCAA 
TCAAAGCTGG ACCGGAATAC AAGTTTTCGC CTGCCTTCTC TTCGTCCTGC TGAGAGTGAC 
AGGTCACGTG GGTTGCCTAA ACTAAAAGAA TCTCGTTCCC ATGAATCTTT GCTGAGCCCT 
GGTAGTGCAG TTGAGGCTCT GGATTTGGGA ACTGAAGATA AAATCCTAGT AAAACCGCTC 
CACAGCAGCA TATTGGGACA AGATTTCTGC TTTGAGGTCA CCTATTCCAG TGGCAGTAAA 
TGTTTCAGCT GCTCATCTGC AGCAGAGAGA GATAAGTGGA TGGAAAATCT TCGTCGAACT 
GTTCAACCAA ACAAGGATAA CTGTCGCCGA GCAGAGAATG TACTACGGCT GTGGATCATT 
GAAGCCAAGG ACCTGGCTCC CAAAAAGAAG TACTTCTGTG AACTTTGTCT GGACGATACT 
CTCTTTGCTC GAACCACAAG CAAGACGAAA GCGGACAATA TTTTTTGGGG GGAATATTTT 
GAGTTTTATG GTTTACCACC CCTGCACAAC ATCACAGTTC ATATTTACAA GGATGTTGAA 
AAGAAGAAAA AAAAAGATAA AAACAATTAT GTTGGTTTAG TGAATATTCC TATGATTAGT 
GTAACTGGGA GGCAGTTTGT GGAGAAATGG TACCCAGTTA GCACCCCCAC TGCAAACAAA 
GGCAAGACTG TTGGTCCTTC CATACGTATA AAGTCCCGTT ACCAAACTAT TACCATTTTA 
CCCATGGAGC AATATAAGGA ATTTGCAGAA TTTATCACAA TTAATTACAC TATGCTATGC 
TCAGTCCTGG AACCAGTCAT TAGTGTCAGA AATAAGGAAG AGATGGCATG TGCTTTAGTG 
CACATCCTGC AGAGTACTGG CAGAGCCAAG GACTTTCTAA CTGATCTGGT CATGTCAGAG 
GTAGATCGTT GTGGGGAGCA CGATGTCTTG ATCTTTAGGG AAAATACACT TGCCACTAAA 
GCCATTGAGG AGTATCTAAA GCTGGTTGGA CAGAAGTACC TTCATGATGC TCTTGGGGAG 
TTTATCAAGG CACTGTATGA GTCGGATGAG AACTGTGAGG TGGATCCCAG CAAATGTTCC 
TCAAGTGAGC TGTCCGACCA CCAGAGCAAC TTAAAAATGT GCTGCGAGCT GGCCTTCTGC 
AAGATAATCA ATTCATATTG TGTTTTTCCC AGAGAGCTGA AGGAAGTGTT TGCTTCTTGG 
AAGCAGCAAT GCATAAATCG GGGAAAGCAG GATATCAGCG AACGACTGAT CAGCGCATCA 
CTCTTCCTTC GCTTTCTTTG TCCTGCAATT ATGTCTCCCA GCCTCTTTGG GCTAATGCAG 
GAGTATCCTG ATGATCGCAC CTCCAGAACT CTCACACTCA TTGCCAAGGT CATCCAGAAT 
CTTGCCAACT TTGCCAAGTT TGGTAATAAA GAAGAATACA TGGCCTTTAT GAATGACTTT 
TTGGAGCATG AGTGGGGTGG AATGAAACGA TTCCTACAAG AGATCTCCAA TCCAGACACC 
ATTTCAAACA CCCCCGGATT TGAGGGTTAC ATAGACCTTG GCCGAGAGCT CTCCGTACTG 
CACAGTCTTT TGTGGGAGGT GGTGTCACAG CTAGATAAGG GTGAAAATTC CTTCCTACAG 
GCGACCGTTG AGAAATTGGG ACCCCTTCCA CGCATTCTTG CTGACATCAC CAAGTCACTG 
ACCAACCCTA CTCCAATCCA ACAGCAGCTA AGACGTTTCA GTGAACATAG CTCCACACCC 
AATGTAAGTG GCAGCCTTTC ATCTGGACTT CAGAAGATTT TTGAGGATCC AGCAGACAGT 
GATTTGCAGA GGTTCAGGTC TCCAACAATT GAAAACCCAG AAAGCTATTT CAGGGGGAAA 
ACGCTACTGC TTGTCCAGCA GGCATCCACA CAGAGTATGT CCTTTTCCGA CAAAGACGAA 
AGAGAGAATG TCTTGCCCAA TGGGCGTAGT GTGTCTTTAA TGGATCTGCA AGACTCCAAT 
GTTAGTCACA CTGATCGTTC CTCAGTTATC CAAGAACCTC CCTTACGTTT AACTGGCAGC 
CAGTTGTCTA TTGCACATGT AGCTACCATT AAACAGCTAC GGGAAAACCA TAGCATGCCT 
CAGAGTGCCC CACAAGTTAG AAAGCCCTTA CATCCAGCAT TGAGCCAGCA AAGCAGCCTG 
CAGCCTCTGT CCTTCCAAAA TCCTGTGTAT CATCTGAACA ACCCACCACC GACAATGCCC 
AAAGCTTCTG TGGACTCCAG CCTTGAAAAT TTAAGCACTG CCAGCTCTAG AAGCCAGAGT 
AATAGTGAAG AGTTCATTCT CAGTGGGCCC AGTAACAGTA GCATGGAAGA TTTCACAAAG 
AGGAGCACTC AGAGTGAGGA CTTTACAAGA AGACACACCA TGCCTGAGAG ACCTATTCCC 
ATAGCACTGC CAAGGCAAAA CAGCACTGGA CAAGCCCAGA TCCGTAAAAC CGACCAGGCT 
GCTCTTGGGG CTCGAGCAAA AGCCCCCCAG TCATTACCAC ACAGTGCCTC ACTGCGGAGC 
GCAGGGAGCA TGTCTGGGAT CTCCAATAAT ATGCTTGTTG AACCAGTGCA GAATGGAAAT 
AGGTCCAGAC AGCAATCTAC ATCATCTCGT GAAAGCCCTG TACCCAAACT AAGAGCAATT 
CACCGTCAGC AGACACAGCA GGTGCAGTCA CCTGTGGACT CCGCTACAAT GTCTCCAGTA 
GAGCGGACTG CAGCCTGGGT TCTTAATAAC GGTCAATATG AAGATGAAGA AGAAACTGTA 
GACCAAAACA AGGATGAATC TAAACCTGCA GAGAAGTATG AACAAGAGAT TGCAAAGCTG 
AAAGAACGAT TGAAAATTTC AAGTCGGCGC CTGGAGGAAT ATGAACGGCG TCTTTTGGTG 
CAAGAGCAGC AAATGCAGAA GCTCTTAATG GAGTACAAAT CTCGACTGGA GGATAGCGAG 
GAGAGGTTGC GCCGTCAGCA GGAAGAGAAG GACAGCCAAA TGAAAAGTGT CATCAGCAGG 
TTAATAGCTG TAGAAGAGGA GCTGAAAAAA GACCATGCTG AGATGCAGGC TGTCATTGAT 
GCAAAGCAAA AAATTATTGA TGCACAGGAG AAGAGGATCA TATCACTGGA TGCAGCCAAC 
ACGCGGCTAA TGAGTGCCCT CACACAGGTC AAGGAACGCT ATAACTCCCA GGTGCGCAAT 
GGAATCTCTC CGACCAACCC TACCAAGCTG TCAATCACTG AAAATGGAGA ATTTAAGAAC 
AGCAGTTGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rasal2 ( XM_004914032.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004914089.1 Xenopus tropicalis RAS protein activator like 2 (rasal2), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OXa57223
Clone ID Related Accession (Same CDS sequence)	XM_004914032.4
Accession Version	XM_004914032.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ras GTPase-activating protein nGAP isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004914032.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCTCA CAGAGGTCCC AGCTGAGAGG TCACCCCGAA GGAGGAGTAT CTCAGGGACT 
AGCACGTCAG ACAAAACCAA TGCTATGGAT CCTGCAAATA CCTCCCCCTT TAAAGTCTCA 
GGCTTCTTTA GTAAACGGTT AAAAGGCTCT ATTAAGAGAA CGAAGAGTCA ATCAAAGCTG 
GACCGGAATA CAAGTTTTCG CCTGCCTTCT CTTCGTCCTG CTGAGAGTGA CAGGTCACGT 
GGGTTGCCTA AACTAAAAGA ATCTCGTTCC CATGAATCTT TGCTGAGCCC TGGTAGTGCA 
GTTGAGGCTC TGGATTTGGG AACTGAAGAT AAAATCCTAG TAAAACCGCT CCACAGCAGC 
ATATTGGGAC AAGATTTCTG CTTTGAGGTC ACCTATTCCA GTGGCAGTAA ATGTTTCAGC 
TGCTCATCTG CAGCAGAGAG AGATAAGTGG ATGGAAAATC TTCGTCGAAC TGTTCAACCA 
AACAAGGATA ACTGTCGCCG AGCAGAGAAT GTACTACGGC TGTGGATCAT TGAAGCCAAG 
GACCTGGCTC CCAAAAAGAA GTACTTCTGT GAACTTTGTC TGGACGATAC TCTCTTTGCT 
CGAACCACAA GCAAGACGAA AGCGGACAAT ATTTTTTGGG GGGAATATTT TGAGTTTTAT 
GGTTTACCAC CCCTGCACAA CATCACAGTT CATATTTACA AGGATGTTGA AAAGAAGAAA 
AAAAAAGATA AAAACAATTA TGTTGGTTTA GTGAATATTC CTATGATTAG TGTAACTGGG 
AGGCAGTTTG TGGAGAAATG GTACCCAGTT AGCACCCCCA CTGCAAACAA AGGCAAGACT 
GTTGGTCCTT CCATACGTAT AAAGTCCCGT TACCAAACTA TTACCATTTT ACCCATGGAG 
CAATATAAGG AATTTGCAGA ATTTATCACA ATTAATTACA CTATGCTATG CTCAGTCCTG 
GAACCAGTCA TTAGTGTCAG AAATAAGGAA GAGATGGCAT GTGCTTTAGT GCACATCCTG 
CAGAGTACTG GCAGAGCCAA GGACTTTCTA ACTGATCTGG TCATGTCAGA GGTAGATCGT 
TGTGGGGAGC ACGATGTCTT GATCTTTAGG GAAAATACAC TTGCCACTAA AGCCATTGAG 
GAGTATCTAA AGCTGGTTGG ACAGAAGTAC CTTCATGATG CTCTTGGGGA GTTTATCAAG 
GCACTGTATG AGTCGGATGA GAACTGTGAG GTGGATCCCA GCAAATGTTC CTCAAGTGAG 
CTGTCCGACC ACCAGAGCAA CTTAAAAATG TGCTGCGAGC TGGCCTTCTG CAAGATAATC 
AATTCATATT GTGTTTTTCC CAGAGAGCTG AAGGAAGTGT TTGCTTCTTG GAAGCAGCAA 
TGCATAAATC GGGGAAAGCA GGATATCAGC GAACGACTGA TCAGCGCATC ACTCTTCCTT 
CGCTTTCTTT GTCCTGCAAT TATGTCTCCC AGCCTCTTTG GGCTAATGCA GGAGTATCCT 
GATGATCGCA CCTCCAGAAC TCTCACACTC ATTGCCAAGG TCATCCAGAA TCTTGCCAAC 
TTTGCCAAGT TTGGTAATAA AGAAGAATAC ATGGCCTTTA TGAATGACTT TTTGGAGCAT 
GAGTGGGGTG GAATGAAACG ATTCCTACAA GAGATCTCCA ATCCAGACAC CATTTCAAAC 
ACCCCCGGAT TTGAGGGTTA CATAGACCTT GGCCGAGAGC TCTCCGTACT GCACAGTCTT 
TTGTGGGAGG TGGTGTCACA GCTAGATAAG GGTGAAAATT CCTTCCTACA GGCGACCGTT 
GAGAAATTGG GACCCCTTCC ACGCATTCTT GCTGACATCA CCAAGTCACT GACCAACCCT 
ACTCCAATCC AACAGCAGCT AAGACGTTTC AGTGAACATA GCTCCACACC CAATGTAAGT 
GGCAGCCTTT CATCTGGACT TCAGAAGATT TTTGAGGATC CAGCAGACAG TGATTTGCAG 
AGGTTCAGGT CTCCAACAAT TGAAAACCCA GAAAGCTATT TCAGGGGGAA AACGCTACTG 
CTTGTCCAGC AGGCATCCAC ACAGAGTATG TCCTTTTCCG ACAAAGACGA AAGAGAGAAT 
GTCTTGCCCA ATGGGCGTAG TGTGTCTTTA ATGGATCTGC AAGACTCCAA TGTTAGTCAC 
ACTGATCGTT CCTCAGTTAT CCAAGAACCT CCCTTACGTT TAACTGGCAG CCAGTTGTCT 
ATTGCACATG TAGCTACCAT TAAACAGCTA CGGGAAAACC ATAGCATGCC TCAGAGTGCC 
CCACAAGTTA GAAAGCCCTT ACATCCAGCA TTGAGCCAGC AAAGCAGCCT GCAGCCTCTG 
TCCTTCCAAA ATCCTGTGTA TCATCTGAAC AACCCACCAC CGACAATGCC CAAAGCTTCT 
GTGGACTCCA GCCTTGAAAA TTTAAGCACT GCCAGCTCTA GAAGCCAGAG TAATAGTGAA 
GAGTTCATTC TCAGTGGGCC CAGTAACAGT AGCATGGAAG ATTTCACAAA GAGGAGCACT 
CAGAGTGAGG ACTTTACAAG AAGACACACC ATGCCTGAGA GACCTATTCC CATAGCACTG 
CCAAGGCAAA ACAGCACTGG ACAAGCCCAG ATCCGTAAAA CCGACCAGGC TGCTCTTGGG 
GCTCGAGCAA AAGCCCCCCA GTCATTACCA CACAGTGCCT CACTGCGGAG CGCAGGGAGC 
ATGTCTGGGA TCTCCAATAA TATGCTTGTT GAACCAGTGC AGAATGGAAA TAGGTCCAGA 
CAGCAATCTA CATCATCTCG TGAAAGCCCT GTACCCAAAC TAAGAGCAAT TCACCGTCAG 
CAGACACAGC AGGTGCAGTC ACCTGTGGAC TCCGCTACAA TGTCTCCAGT AGAGCGGACT 
GCAGCCTGGG TTCTTAATAA CGGTCAATAT GAAGATGAAG AAGAAACTGT AGACCAAAAC 
AAGGATGAAT CTAAACCTGC AGAGAAGTAT GAACAAGAGA TTGCAAAGCT GAAAGAACGA 
TTGAAAATTT CAAGTCGGCG CCTGGAGGAA TATGAACGGC GTCTTTTGGT GCAAGAGCAG 
CAAATGCAGA AGCTCTTAAT GGAGTACAAA TCTCGACTGG AGGATAGCGA GGAGAGGTTG 
CGCCGTCAGC AGGAAGAGAA GGACAGCCAA ATGAAAAGTG TCATCAGCAG GTTAATAGCT 
GTAGAAGAGG AGCTGAAAAA AGACCATGCT GAGATGCAGG CTGTCATTGA TGCAAAGCAA 
AAAATTATTG ATGCACAGGA GAAGAGGATC ATATCACTGG ATGCAGCCAA CACGCGGCTA 
ATGAGTGCCC TCACACAGGT CAAGGAACGC TATAACTCCC AGGTGCGCAA TGGAATCTCT 
CCGACCAACC CTACCAAGCT GTCAATCACT GAAAATGGAG AATTTAAGAA CAGCAGTTGT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004914089.1
CDS	90..3512
Translation	MQLTEVPAERSPRRRSISGTSTSDKTNAMDPANTSPFKVSGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRPAESDRSRGLPKLKESRSHESLLSPGSAVEALDLGTEDKILVKPLHSSILGQDFCFEVTYSSGSKCFSCSSAAERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEYFEFYGLPPLHNITVHIYKDVEKKKKKDKNNYVGLVNIPMISVTGRQFVEKWYPVSTPTANKGKTVGPSIRIKSRYQTITILPMEQYKEFAEFITINYTMLCSVLEPVISVRNKEEMACALVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTLATKAIEEYLKLVGQKYLHDALGEFIKALYESDENCEVDPSKCSSSELSDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCINRGKQDISERLISASLFLRFLCPAIMSPSLFGLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLQEISNPDTISNTPGFEGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVEKLGPLPRILADITKSLTNPTPIQQQLRRFSEHSSTPNVSGSLSSGLQKIFEDPADSDLQRFRSPTIENPESYFRGKTLLLVQQASTQSMSFSDKDERENVLPNGRSVSLMDLQDSNVSHTDRSSVIQEPPLRLTGSQLSIAHVATIKQLRENHSMPQSAPQVRKPLHPALSQQSSLQPLSFQNPVYHLNNPPPTMPKASVDSSLENLSTASSRSQSNSEEFILSGPSNSSMEDFTKRSTQSEDFTRRHTMPERPIPIALPRQNSTGQAQIRKTDQAALGARAKAPQSLPHSASLRSAGSMSGISNNMLVEPVQNGNRSRQQSTSSRESPVPKLRAIHRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEDEEETVDQNKDESKPAEKYEQEIAKLKERLKISSRRLEEYERRLLVQEQQMQKLLMEYKSRLEDSEERLRRQQEEKDSQMKSVISRLIAVEEELKKDHAEMQAVIDAKQKIIDAQEKRIISLDAANTRLMSALTQVKERYNSQVRNGISPTNPTKLSITENGEFKNSSC

Target ORF information:

RefSeq Version	XM_004914032.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis RAS protein activator like 2 (rasal2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004914032.4

ORF Insert Sequence:

ATGCAGCTCA CAGAGGTCCC AGCTGAGAGG TCACCCCGAA GGAGGAGTAT CTCAGGGACT 
AGCACGTCAG ACAAAACCAA TGCTATGGAT CCTGCAAATA CCTCCCCCTT TAAAGTCTCA 
GGCTTCTTTA GTAAACGGTT AAAAGGCTCT ATTAAGAGAA CGAAGAGTCA ATCAAAGCTG 
GACCGGAATA CAAGTTTTCG CCTGCCTTCT CTTCGTCCTG CTGAGAGTGA CAGGTCACGT 
GGGTTGCCTA AACTAAAAGA ATCTCGTTCC CATGAATCTT TGCTGAGCCC TGGTAGTGCA 
GTTGAGGCTC TGGATTTGGG AACTGAAGAT AAAATCCTAG TAAAACCGCT CCACAGCAGC 
ATATTGGGAC AAGATTTCTG CTTTGAGGTC ACCTATTCCA GTGGCAGTAA ATGTTTCAGC 
TGCTCATCTG CAGCAGAGAG AGATAAGTGG ATGGAAAATC TTCGTCGAAC TGTTCAACCA 
AACAAGGATA ACTGTCGCCG AGCAGAGAAT GTACTACGGC TGTGGATCAT TGAAGCCAAG 
GACCTGGCTC CCAAAAAGAA GTACTTCTGT GAACTTTGTC TGGACGATAC TCTCTTTGCT 
CGAACCACAA GCAAGACGAA AGCGGACAAT ATTTTTTGGG GGGAATATTT TGAGTTTTAT 
GGTTTACCAC CCCTGCACAA CATCACAGTT CATATTTACA AGGATGTTGA AAAGAAGAAA 
AAAAAAGATA AAAACAATTA TGTTGGTTTA GTGAATATTC CTATGATTAG TGTAACTGGG 
AGGCAGTTTG TGGAGAAATG GTACCCAGTT AGCACCCCCA CTGCAAACAA AGGCAAGACT 
GTTGGTCCTT CCATACGTAT AAAGTCCCGT TACCAAACTA TTACCATTTT ACCCATGGAG 
CAATATAAGG AATTTGCAGA ATTTATCACA ATTAATTACA CTATGCTATG CTCAGTCCTG 
GAACCAGTCA TTAGTGTCAG AAATAAGGAA GAGATGGCAT GTGCTTTAGT GCACATCCTG 
CAGAGTACTG GCAGAGCCAA GGACTTTCTA ACTGATCTGG TCATGTCAGA GGTAGATCGT 
TGTGGGGAGC ACGATGTCTT GATCTTTAGG GAAAATACAC TTGCCACTAA AGCCATTGAG 
GAGTATCTAA AGCTGGTTGG ACAGAAGTAC CTTCATGATG CTCTTGGGGA GTTTATCAAG 
GCACTGTATG AGTCGGATGA GAACTGTGAG GTGGATCCCA GCAAATGTTC CTCAAGTGAG 
CTGTCCGACC ACCAGAGCAA CTTAAAAATG TGCTGCGAGC TGGCCTTCTG CAAGATAATC 
AATTCATATT GTGTTTTTCC CAGAGAGCTG AAGGAAGTGT TTGCTTCTTG GAAGCAGCAA 
TGCATAAATC GGGGAAAGCA GGATATCAGC GAACGACTGA TCAGCGCATC ACTCTTCCTT 
CGCTTTCTTT GTCCTGCAAT TATGTCTCCC AGCCTCTTTG GGCTAATGCA GGAGTATCCT 
GATGATCGCA CCTCCAGAAC TCTCACACTC ATTGCCAAGG TCATCCAGAA TCTTGCCAAC 
TTTGCCAAGT TTGGTAATAA AGAAGAATAC ATGGCCTTTA TGAATGACTT TTTGGAGCAT 
GAGTGGGGTG GAATGAAACG ATTCCTACAA GAGATCTCCA ATCCAGACAC CATTTCAAAC 
ACCCCCGGAT TTGAGGGTTA CATAGACCTT GGCCGAGAGC TCTCCGTACT GCACAGTCTT 
TTGTGGGAGG TGGTGTCACA GCTAGATAAG GGTGAAAATT CCTTCCTACA GGCGACCGTT 
GAGAAATTGG GACCCCTTCC ACGCATTCTT GCTGACATCA CCAAGTCACT GACCAACCCT 
ACTCCAATCC AACAGCAGCT AAGACGTTTC AGTGAACATA GCTCCACACC CAATGTAAGT 
GGCAGCCTTT CATCTGGACT TCAGAAGATT TTTGAGGATC CAGCAGACAG TGATTTGCAG 
AGGTTCAGGT CTCCAACAAT TGAAAACCCA GAAAGCTATT TCAGGGGGAA AACGCTACTG 
CTTGTCCAGC AGGCATCCAC ACAGAGTATG TCCTTTTCCG ACAAAGACGA AAGAGAGAAT 
GTCTTGCCCA ATGGGCGTAG TGTGTCTTTA ATGGATCTGC AAGACTCCAA TGTTAGTCAC 
ACTGATCGTT CCTCAGTTAT CCAAGAACCT CCCTTACGTT TAACTGGCAG CCAGTTGTCT 
ATTGCACATG TAGCTACCAT TAAACAGCTA CGGGAAAACC ATAGCATGCC TCAGAGTGCC 
CCACAAGTTA GAAAGCCCTT ACATCCAGCA TTGAGCCAGC AAAGCAGCCT GCAGCCTCTG 
TCCTTCCAAA ATCCTGTGTA TCATCTGAAC AACCCACCAC CGACAATGCC CAAAGCTTCT 
GTGGACTCCA GCCTTGAAAA TTTAAGCACT GCCAGCTCTA GAAGCCAGAG TAATAGTGAA 
GAGTTCATTC TCAGTGGGCC CAGTAACAGT AGCATGGAAG ATTTCACAAA GAGGAGCACT 
CAGAGTGAGG ACTTTACAAG AAGACACACC ATGCCTGAGA GACCTATTCC CATAGCACTG 
CCAAGGCAAA ACAGCACTGG ACAAGCCCAG ATCCGTAAAA CCGACCAGGC TGCTCTTGGG 
GCTCGAGCAA AAGCCCCCCA GTCATTACCA CACAGTGCCT CACTGCGGAG CGCAGGGAGC 
ATGTCTGGGA TCTCCAATAA TATGCTTGTT GAACCAGTGC AGAATGGAAA TAGGTCCAGA 
CAGCAATCTA CATCATCTCG TGAAAGCCCT GTACCCAAAC TAAGAGCAAT TCACCGTCAG 
CAGACACAGC AGGTGCAGTC ACCTGTGGAC TCCGCTACAA TGTCTCCAGT AGAGCGGACT 
GCAGCCTGGG TTCTTAATAA CGGTCAATAT GAAGATGAAG AAGAAACTGT AGACCAAAAC 
AAGGATGAAT CTAAACCTGC AGAGAAGTAT GAACAAGAGA TTGCAAAGCT GAAAGAACGA 
TTGAAAATTT CAAGTCGGCG CCTGGAGGAA TATGAACGGC GTCTTTTGGT GCAAGAGCAG 
CAAATGCAGA AGCTCTTAAT GGAGTACAAA TCTCGACTGG AGGATAGCGA GGAGAGGTTG 
CGCCGTCAGC AGGAAGAGAA GGACAGCCAA ATGAAAAGTG TCATCAGCAG GTTAATAGCT 
GTAGAAGAGG AGCTGAAAAA AGACCATGCT GAGATGCAGG CTGTCATTGA TGCAAAGCAA 
AAAATTATTG ATGCACAGGA GAAGAGGATC ATATCACTGG ATGCAGCCAA CACGCGGCTA 
ATGAGTGCCC TCACACAGGT CAAGGAACGC TATAACTCCC AGGTGCGCAA TGGAATCTCT 
CCGACCAACC CTACCAAGCT GTCAATCACT GAAAATGGAG AATTTAAGAA CAGCAGTTGT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rasal2 ( XM_004914030.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004914087.1 Xenopus tropicalis RAS protein activator like 2 (rasal2), transcript variant X1, mRNA.

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CloneID	OXa09553
Clone ID Related Accession (Same CDS sequence)	XM_004914030.3
Accession Version	XM_004914030.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3840bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ras GTPase-activating protein nGAP isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004914030.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCATGA TGGATTTATC CCCCGGCGGG GATTCGGTAA CATCCGGACA TGACTTCTTC 
CAGGACAGAG ATTCCCTCCC AGAGGATTTG GACTCTCCCG GGGGAGTGGG GGGGATTCTG 
GACAGGATCC TAATGGAGTC TGTTTGCCAG CAGCAGGGAT GGGTGCGAGT ATACGATGTG 
AAAGGCCCGC CTACACACAG GCTGTCGTGT GGTCAGTCGC CCTACACTGA AAGCACAACA 
TGGGAGCGCA AGTACTGTAT CCTAACAGAA AGCCAACTGG TGCTCCTTAA CCGTGACGAT 
GAGATGCCTG GGGAAACTGC ACAGGACCCA CCTCCAGAAT CCAGCAAAAG CAGAAGCCTG 
CGTAGGACTG TGAGTGTCCC GTCAGAAGGG CAGTTTCCAG ACTACCCCCC GGAGGGTGCT 
GCCAAGCTGG AGGTCCCAGC TGAGAGGTCA CCCCGAAGGA GGAGTATCTC AGGGACTAGC 
ACGTCAGACA AAACCAATGC TATGGATCCT GCAAATACCT CCCCCTTTAA AGTCTCAGGC 
TTCTTTAGTA AACGGTTAAA AGGCTCTATT AAGAGAACGA AGAGTCAATC AAAGCTGGAC 
CGGAATACAA GTTTTCGCCT GCCTTCTCTT CGTCCTGCTG AGAGTGACCG GTCACGTGGG 
TTGCCTAAAC TAAAAGAATC TCGTTCCCAT GAATCTTTGC TGAGCCCTGG TAGTGCAGTT 
GAGGCTCTGG ATTTGGGAAC TGAAGATAAA ATCCTAGTAA AACCGCTCCA CAGCAGCATA 
TTGGGACAAG ATTTCTGCTT TGAGGTCACC TATTCCAGTG GCAGTAAATG TTTCAGCTGC 
TCATCTGCAG CAGAGAGAGA TAAGTGGATG GAAAATCTTC GTCGAACTGT TCAACCAAAC 
AAGGATAACT GTCGCCGAGC AGAGAATGTA CTACGGCTGT GGATCATTGA AGCCAAGGAC 
CTGGCTCCCA AAAAGAAGTA CTTCTGTGAA CTTTGTCTGG ACGATACTCT CTTTGCTCGA 
ACCACAAGCA AGACGAAAGC GGACAATATT TTTTGGGGGG AATATTTTGA GTTTTATGGT 
TTACCACCCC TGCACAACAT CACAGTTCAT ATTTACAAGG ATGTTGAAAA GAAGAAAAAA 
AAAGATAAAA ACAATTATGT TGGTTTAGTG AATATTCCTA TGATTAGTGT AACTGGGAGG 
CAGTTTGTGG AGAAATGGTA CCCAGTTAGC ACCCCCACTG CAAACAAAGG CAAGACTGTT 
GGTCCTTCCA TACGTATAAA GTCCCGTTAC CAAACTATTA CCATTTTACC CATGGAGCAA 
TATAAGGAAT TTGCAGAATT TATCACAATT AATTACACTA TGCTATGCTC AGTCCTGGAA 
CCAGTCATTA GTGTCAGAAA TAAGGAAGAG ATGGCATGTG CTTTAGTGCA CATCCTGCAG 
AGTACTGGCA GAGCCAAGGA CTTTCTAACT GATCTGGTGA TGTCAGAGGT AGATCGTTGT 
GGGGAGCACG ATGTCTTGAT CTTTAGGGAA AATACACTTG CCACTAAAGC CATTGAGGAG 
TATCTAAAGC TGGTTGGACA GAAGTACCTT CATGATGCTC TTGGGGAGTT TATCAAGGCA 
CTGTATGAGT CGGATGAGAA CTGTGAGGTG GATCCCAGCA AATGTTCCTC AAGTGAGCTG 
TCCGACCACC AGAGCAACTT AAAAATGTGC TGCGAGCTGG CCTTCTGCAA GATAATCAAT 
TCATATTGTG TTTTTCCCAG AGAGCTGAAG GAAGTGTTTG CTTCTTGGAA GCAGCAATGC 
ATAAATCGGG GAAAGCAGGA TATCAGCGAA CGACTGATCA GCGCATCACT CTTCCTTCGC 
TTTCTTTGTC CTGCAATTAT GTCTCCCAGC CTCTTTGGGC TAATGCAGGA GTATCCTGAT 
GATCGCACCT CCAGAACTCT CACACTCATT GCCAAGGTCA TCCAGAATCT TGCCAACTTT 
GCCAAGTTTG GTAATAAAGA AGAATACATG GCCTTTATGA ATGACTTTTT GGAGCATGAG 
TGGGGTGGAA TGAAACGATT CCTACAAGAG ATCTCCAATC CAGACACCAT TTCAAACACC 
CCCGGATTTG AGGGTTACAT AGACCTTGGC CGAGAGCTCT CCGTACTGCA CAGTCTTTTG 
TGGGAGGTGG TGTCACAGCT AGATAAGGGT GAAAATTCCT TCCTACAGGC GACCGTTGAG 
AAATTGGGAC CCCTTCCACG CATTCTTGCT GACATCACCA AGTCACTGAC CAACCCTACT 
CCAATCCAAC AGCAGCTAAG ACGTTTCAGT GAACATAGCT CCACACCCAA TGTAAGTGGC 
AGCCTTTCAT CTGGACTTCA GAAGATTTTT GAGGATCCAG CAGACAGTGA TTTGCAGAGG 
TTCAGGTCTC CAACAATTGA AAACCCAGAA AGCTATTTCA GGGGGAAAAC GCTACTGCTT 
GTCCAGCAGG CATCCACACA GAGTATGTCC TTTTCCGACA AAGACGAAAG AGAGAATGTC 
TTGCCCAATG GGCGTAGTGT GTCTTTAATG GATCTGCAAG ACTCCAATGT TAGTCACACT 
GATCGTTCCT CAGTTATCCA AGAACCTCCC TTACGTTTAA CTGGCAGCCA GTTGTCTATT 
GCACATGTAG CTACCATTAA ACAGCTACGG GAAAACCATA GCATGCCTCA GAGTGCCCCA 
CAAGTTAGAA AGCCCTTACA TCCAGCATTG AGCCAGCAAA GCAGCCTGCA GCCTCTGTCC 
TTCCAAAATC CTGTGTATCA TCTGAACAAC CCACCACCGA CAATGCCCAA AGCTTCTGTG 
GACTCCAGCC TTGAAAATTT AAGCACTGCC AGCTCTAGAA GCCAGAGTAA TAGTGAAGAG 
TTCATTCTCA GTGGGCCCAG TAACAGTAGC ATGGAAGATT TCACAAAGAG GAGCACTCAG 
AGTGAGGACT TTACAAGAAG ACACACCATG CCTGAGAGAC CTATTCCCAT AGCACTGCCA 
AGGCAAAACA GCACTGGACA AGCCCAGATC CGTAAAACCG ACCAGGCTGC TCTTGGGGCT 
CGAGCAAAAG CCCCCCAGTC ATTACCACAC AGTGCCTCAC TGCGGAGCGC AGGGAGCATG 
TCTGGGATCT CCAATAATAT GCTTGTTGAA CCAGTGCAGA ATGGAAATAG GTCCAGACAG 
CAATCTACAT CATCTCGTGA AAGCCCTGTA CCCAAACTAA GAGCAATTCA CCGTCAGCAG 
ACACAGCAGG TGCAGTCACC TGTGGACTCC GCTACAATGT CTCCAGTAGA GCGGACTGCA 
GCCTGGGTTC TTAATAACGG TCAATATGAA GATGAAGAAG AAACTGTAGA CCAAAACAAG 
GATGAATCTA AACCTGCAGA GAAGTATGAA CAAGAGATTG CAAAGCTGAA AGAACGATTG 
AAAATTTCAA GTCGGCGCCT GGAGGAATAT GAACGGCGTC TTTTGGTGCA AGAGCAGCAA 
ATGCAGAAGC TCTTAATGGA GTACAAATCT CGACTGGAGG ATAGCGAGGA GAGGTTGCGC 
CGTCAGCAGG AAGAGAAGGA CAGCCAAATG AAAAGTGTCA TCAGCAGGTT AATAGCTGTA 
GAAGAGGAGC TGAAAAAAGA CCATGCTGAG ATGCAGGCTG TCATTGATGC AAAGCAAAAA 
ATTATTGATG CACAGGAGAA GAGGATCATA TCACTGGATG CAGCCAACAC GCGGCTAATG 
AGTGCCCTCA CACAGGTCAA GGAACGCTAT AACTCCCAGG TGCGCAATGG AATCTCTCCG 
ACCAACCCTA CCAAGCTGTC AATCACTGAA AATGGAGAAT TTAAGAACAG CAGTTGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004914087.1
CDS	659..4498
Translation	MLMMDLSPGGDSVTSGHDFFQDRDSLPEDLDSPGGVGGILDRILMESVCQQQGWVRVYDVKGPPTHRLSCGQSPYTESTTWERKYCILTESQLVLLNRDDEMPGETAQDPPPESSKSRSLRRTVSVPSEGQFPDYPPEGAAKLEVPAERSPRRRSISGTSTSDKTNAMDPANTSPFKVSGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRPAESDRSRGLPKLKESRSHESLLSPGSAVEALDLGTEDKILVKPLHSSILGQDFCFEVTYSSGSKCFSCSSAAERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEYFEFYGLPPLHNITVHIYKDVEKKKKKDKNNYVGLVNIPMISVTGRQFVEKWYPVSTPTANKGKTVGPSIRIKSRYQTITILPMEQYKEFAEFITINYTMLCSVLEPVISVRNKEEMACALVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTLATKAIEEYLKLVGQKYLHDALGEFIKALYESDENCEVDPSKCSSSELSDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCINRGKQDISERLISASLFLRFLCPAIMSPSLFGLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLQEISNPDTISNTPGFEGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVEKLGPLPRILADITKSLTNPTPIQQQLRRFSEHSSTPNVSGSLSSGLQKIFEDPADSDLQRFRSPTIENPESYFRGKTLLLVQQASTQSMSFSDKDERENVLPNGRSVSLMDLQDSNVSHTDRSSVIQEPPLRLTGSQLSIAHVATIKQLRENHSMPQSAPQVRKPLHPALSQQSSLQPLSFQNPVYHLNNPPPTMPKASVDSSLENLSTASSRSQSNSEEFILSGPSNSSMEDFTKRSTQSEDFTRRHTMPERPIPIALPRQNSTGQAQIRKTDQAALGARAKAPQSLPHSASLRSAGSMSGISNNMLVEPVQNGNRSRQQSTSSRESPVPKLRAIHRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEDEEETVDQNKDESKPAEKYEQEIAKLKERLKISSRRLEEYERRLLVQEQQMQKLLMEYKSRLEDSEERLRRQQEEKDSQMKSVISRLIAVEEELKKDHAEMQAVIDAKQKIIDAQEKRIISLDAANTRLMSALTQVKERYNSQVRNGISPTNPTKLSITENGEFKNSSC

Target ORF information:

RefSeq Version	XM_004914030.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis RAS protein activator like 2 (rasal2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004914030.3

ORF Insert Sequence:

ATGCTCATGA TGGATTTATC CCCCGGCGGG GATTCGGTAA CATCCGGACA TGACTTCTTC 
CAGGACAGAG ATTCCCTCCC AGAGGATTTG GACTCTCCCG GGGGAGTGGG GGGGATTCTG 
GACAGGATCC TAATGGAGTC TGTTTGCCAG CAGCAGGGAT GGGTGCGAGT ATACGATGTG 
AAAGGCCCGC CTACACACAG GCTGTCGTGT GGTCAGTCGC CCTACACTGA AAGCACAACA 
TGGGAGCGCA AGTACTGTAT CCTAACAGAA AGCCAACTGG TGCTCCTTAA CCGTGACGAT 
GAGATGCCTG GGGAAACTGC ACAGGACCCA CCTCCAGAAT CCAGCAAAAG CAGAAGCCTG 
CGTAGGACTG TGAGTGTCCC GTCAGAAGGG CAGTTTCCAG ACTACCCCCC GGAGGGTGCT 
GCCAAGCTGG AGGTCCCAGC TGAGAGGTCA CCCCGAAGGA GGAGTATCTC AGGGACTAGC 
ACGTCAGACA AAACCAATGC TATGGATCCT GCAAATACCT CCCCCTTTAA AGTCTCAGGC 
TTCTTTAGTA AACGGTTAAA AGGCTCTATT AAGAGAACGA AGAGTCAATC AAAGCTGGAC 
CGGAATACAA GTTTTCGCCT GCCTTCTCTT CGTCCTGCTG AGAGTGACCG GTCACGTGGG 
TTGCCTAAAC TAAAAGAATC TCGTTCCCAT GAATCTTTGC TGAGCCCTGG TAGTGCAGTT 
GAGGCTCTGG ATTTGGGAAC TGAAGATAAA ATCCTAGTAA AACCGCTCCA CAGCAGCATA 
TTGGGACAAG ATTTCTGCTT TGAGGTCACC TATTCCAGTG GCAGTAAATG TTTCAGCTGC 
TCATCTGCAG CAGAGAGAGA TAAGTGGATG GAAAATCTTC GTCGAACTGT TCAACCAAAC 
AAGGATAACT GTCGCCGAGC AGAGAATGTA CTACGGCTGT GGATCATTGA AGCCAAGGAC 
CTGGCTCCCA AAAAGAAGTA CTTCTGTGAA CTTTGTCTGG ACGATACTCT CTTTGCTCGA 
ACCACAAGCA AGACGAAAGC GGACAATATT TTTTGGGGGG AATATTTTGA GTTTTATGGT 
TTACCACCCC TGCACAACAT CACAGTTCAT ATTTACAAGG ATGTTGAAAA GAAGAAAAAA 
AAAGATAAAA ACAATTATGT TGGTTTAGTG AATATTCCTA TGATTAGTGT AACTGGGAGG 
CAGTTTGTGG AGAAATGGTA CCCAGTTAGC ACCCCCACTG CAAACAAAGG CAAGACTGTT 
GGTCCTTCCA TACGTATAAA GTCCCGTTAC CAAACTATTA CCATTTTACC CATGGAGCAA 
TATAAGGAAT TTGCAGAATT TATCACAATT AATTACACTA TGCTATGCTC AGTCCTGGAA 
CCAGTCATTA GTGTCAGAAA TAAGGAAGAG ATGGCATGTG CTTTAGTGCA CATCCTGCAG 
AGTACTGGCA GAGCCAAGGA CTTTCTAACT GATCTGGTGA TGTCAGAGGT AGATCGTTGT 
GGGGAGCACG ATGTCTTGAT CTTTAGGGAA AATACACTTG CCACTAAAGC CATTGAGGAG 
TATCTAAAGC TGGTTGGACA GAAGTACCTT CATGATGCTC TTGGGGAGTT TATCAAGGCA 
CTGTATGAGT CGGATGAGAA CTGTGAGGTG GATCCCAGCA AATGTTCCTC AAGTGAGCTG 
TCCGACCACC AGAGCAACTT AAAAATGTGC TGCGAGCTGG CCTTCTGCAA GATAATCAAT 
TCATATTGTG TTTTTCCCAG AGAGCTGAAG GAAGTGTTTG CTTCTTGGAA GCAGCAATGC 
ATAAATCGGG GAAAGCAGGA TATCAGCGAA CGACTGATCA GCGCATCACT CTTCCTTCGC 
TTTCTTTGTC CTGCAATTAT GTCTCCCAGC CTCTTTGGGC TAATGCAGGA GTATCCTGAT 
GATCGCACCT CCAGAACTCT CACACTCATT GCCAAGGTCA TCCAGAATCT TGCCAACTTT 
GCCAAGTTTG GTAATAAAGA AGAATACATG GCCTTTATGA ATGACTTTTT GGAGCATGAG 
TGGGGTGGAA TGAAACGATT CCTACAAGAG ATCTCCAATC CAGACACCAT TTCAAACACC 
CCCGGATTTG AGGGTTACAT AGACCTTGGC CGAGAGCTCT CCGTACTGCA CAGTCTTTTG 
TGGGAGGTGG TGTCACAGCT AGATAAGGGT GAAAATTCCT TCCTACAGGC GACCGTTGAG 
AAATTGGGAC CCCTTCCACG CATTCTTGCT GACATCACCA AGTCACTGAC CAACCCTACT 
CCAATCCAAC AGCAGCTAAG ACGTTTCAGT GAACATAGCT CCACACCCAA TGTAAGTGGC 
AGCCTTTCAT CTGGACTTCA GAAGATTTTT GAGGATCCAG CAGACAGTGA TTTGCAGAGG 
TTCAGGTCTC CAACAATTGA AAACCCAGAA AGCTATTTCA GGGGGAAAAC GCTACTGCTT 
GTCCAGCAGG CATCCACACA GAGTATGTCC TTTTCCGACA AAGACGAAAG AGAGAATGTC 
TTGCCCAATG GGCGTAGTGT GTCTTTAATG GATCTGCAAG ACTCCAATGT TAGTCACACT 
GATCGTTCCT CAGTTATCCA AGAACCTCCC TTACGTTTAA CTGGCAGCCA GTTGTCTATT 
GCACATGTAG CTACCATTAA ACAGCTACGG GAAAACCATA GCATGCCTCA GAGTGCCCCA 
CAAGTTAGAA AGCCCTTACA TCCAGCATTG AGCCAGCAAA GCAGCCTGCA GCCTCTGTCC 
TTCCAAAATC CTGTGTATCA TCTGAACAAC CCACCACCGA CAATGCCCAA AGCTTCTGTG 
GACTCCAGCC TTGAAAATTT AAGCACTGCC AGCTCTAGAA GCCAGAGTAA TAGTGAAGAG 
TTCATTCTCA GTGGGCCCAG TAACAGTAGC ATGGAAGATT TCACAAAGAG GAGCACTCAG 
AGTGAGGACT TTACAAGAAG ACACACCATG CCTGAGAGAC CTATTCCCAT AGCACTGCCA 
AGGCAAAACA GCACTGGACA AGCCCAGATC CGTAAAACCG ACCAGGCTGC TCTTGGGGCT 
CGAGCAAAAG CCCCCCAGTC ATTACCACAC AGTGCCTCAC TGCGGAGCGC AGGGAGCATG 
TCTGGGATCT CCAATAATAT GCTTGTTGAA CCAGTGCAGA ATGGAAATAG GTCCAGACAG 
CAATCTACAT CATCTCGTGA AAGCCCTGTA CCCAAACTAA GAGCAATTCA CCGTCAGCAG 
ACACAGCAGG TGCAGTCACC TGTGGACTCC GCTACAATGT CTCCAGTAGA GCGGACTGCA 
GCCTGGGTTC TTAATAACGG TCAATATGAA GATGAAGAAG AAACTGTAGA CCAAAACAAG 
GATGAATCTA AACCTGCAGA GAAGTATGAA CAAGAGATTG CAAAGCTGAA AGAACGATTG 
AAAATTTCAA GTCGGCGCCT GGAGGAATAT GAACGGCGTC TTTTGGTGCA AGAGCAGCAA 
ATGCAGAAGC TCTTAATGGA GTACAAATCT CGACTGGAGG ATAGCGAGGA GAGGTTGCGC 
CGTCAGCAGG AAGAGAAGGA CAGCCAAATG AAAAGTGTCA TCAGCAGGTT AATAGCTGTA 
GAAGAGGAGC TGAAAAAAGA CCATGCTGAG ATGCAGGCTG TCATTGATGC AAAGCAAAAA 
ATTATTGATG CACAGGAGAA GAGGATCATA TCACTGGATG CAGCCAACAC GCGGCTAATG 
AGTGCCCTCA CACAGGTCAA GGAACGCTAT AACTCCCAGG TGCGCAATGG AATCTCTCCG 
ACCAACCCTA CCAAGCTGTC AATCACTGAA AATGGAGAAT TTAAGAACAG CAGTTGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rasal2 ( XM_002937360.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002937406.1 Xenopus tropicalis RAS protein activator like 2 (rasal2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa09554
Clone ID Related Accession (Same CDS sequence)	XM_002937360.4
Accession Version	XM_002937360.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3819bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ras GTPase-activating protein nGAP isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002937360.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCATGA TGGATTTATC CCCCGGCGGG GATTCGGTAA CATCCGGACA TGACTTCTTC 
CAGGACAGAG ATTCCCTCCC AGAGGATTTG GACTCTCCCG GGGGAGTGGG GGGGATTCTG 
GACAGGATCC TAATGGAGTC TGTTTGCCAG CAGCAGGGAT GGGTGCGAGT ATACGATGTG 
AAAGGCCCGC CTACACACAG GCTGTCGTGT GGTCAGTCGC CCTACACTGA AAGCACAACA 
TGGGAGCGCA AGTACTGTAT CCTAACAGAA AGCCAACTGG TGCTCCTTAA CCGTGACGAT 
GAGATGCCTG GGGAAACTGC ACAGGACCCA CCTCCAGAAT CCAGCAAAAG CAGAAGCCTG 
CGTAGGACTG TGAGTGTCCC GTCAGAAGGG CAGTTTCCAG ACTACCCCCC GGAGGGTGCT 
GCCAAGCTGG AGGTCCCAGC TGAGAGGTCA CCCCGAAGGA GGAGTATCTC AGGGACTAGC 
ACGTCAGACA AAACCAATGC TATGGATCCT GCAAATACCT CCCCCTTTAA AGTCTCAGGC 
TTCTTTAGTA AACGGTTAAA AGGCTCTATT AAGAGAACGA AGAGTCAATC AAAGCTGGAC 
CGGAATACAA GTTTTCGCCT GCCTTCTCTT CGTCCTGCTG AGAGTGACCG GTCACGTGGG 
TTGCCTAAAC TAAAAGAATC TCGTTCCCAT GAATCTTTGC TGAGCCCTGG TAGTGCAGTT 
GAGGCTCTGG ATTTGGGAAC TGAAGATAAA ATCCTAGTAA AACCGCTCCA CAGCAGCATA 
TTGGGACAAG ATTTCTGCTT TGAGGTCACC TATTCCAGTG GCAGTAAATG TTTCAGCTGC 
TCATCTGCAG CAGAGAGAGA TAAGTGGATG GAAAATCTTC GTCGAACTGT TCAACCAAAC 
AAGGATAACT GTCGCCGAGC AGAGAATGTA CTACGGCTGT GGATCATTGA AGCCAAGGAC 
CTGGCTCCCA AAAAGAAGTA CTTCTGTGAA CTTTGTCTGG ACGATACTCT CTTTGCTCGA 
ACCACAAGCA AGACGAAAGC GGACAATATT TTTTGGGGGG AATATTTTGA GTTTTATGGT 
TTACCACCCC TGCACAACAT CACAGTTCAT ATTTACAAGG ATGTTGAAAA GAAGAAAAAA 
AAAGATAAAA ACAATTATGT TGGTTTAGTG AATATTCCTA TGATTAGTGT AACTGGGAGG 
CAGTTTGTGG AGAAATGGTA CCCAGTTAGC ACCCCCACTG CAAACAAAGG CAAGACTGTT 
GGTCCTTCCA TACGTATAAA GTCCCGTTAC CAAACTATTA CCATTTTACC CATGGAGCAA 
TATAAGGAAT TTGCAGAATT TATCACAATT AATTACACTA TGCTATGCTC AGTCCTGGAA 
CCAGTCATTA GTGTCAGAAA TAAGGAAGAG ATGGCATGTG CTTTAGTGCA CATCCTGCAG 
AGTACTGGCA GAGCCAAGGA CTTTCTAACT GATCTGGTGA TGTCAGAGGT AGATCGTTGT 
GGGGAGCACG ATGTCTTGAT CTTTAGGGAA AATACACTTG CCACTAAAGC CATTGAGGAG 
TATCTAAAGC TGGTTGGACA GAAGTACCTT CATGATGCTC TTGGGGAGTT TATCAAGGCA 
CTGTATGAGT CGGATGAGAA CTGTGAGGTG GATCCCAGCA AATGTTCCTC AAGTGAGCTG 
TCCGACCACC AGAGCAACTT AAAAATGTGC TGCGAGCTGG CCTTCTGCAA GATAATCAAT 
TCATATTGTG TTTTTCCCAG AGAGCTGAAG GAAGTGTTTG CTTCTTGGAA GCAGCAATGC 
ATAAATCGGG GAAAGCAGGA TATCAGCGAA CGACTGATCA GCGCATCACT CTTCCTTCGC 
TTTCTTTGTC CTGCAATTAT GTCTCCCAGC CTCTTTGGGC TAATGCAGGA GTATCCTGAT 
GATCGCACCT CCAGAACTCT CACACTCATT GCCAAGGTCA TCCAGAATCT TGCCAACTTT 
GCCAAGTTTG GTAATAAAGA AGAATACATG GCCTTTATGA ATGACTTTTT GGAGCATGAG 
TGGGGTGGAA TGAAACGATT CCTACAAGAG ATCTCCAATC CAGACACCAT TTCAAACACC 
CCCGGATTTG AGGGTTACAT AGACCTTGGC CGAGAGCTCT CCGTACTGCA CAGTCTTTTG 
TGGGAGGTGG TGTCACAGCT AGATAAGGCG ACCGTTGAGA AATTGGGACC CCTTCCACGC 
ATTCTTGCTG ACATCACCAA GTCACTGACC AACCCTACTC CAATCCAACA GCAGCTAAGA 
CGTTTCAGTG AACATAGCTC CACACCCAAT GTAAGTGGCA GCCTTTCATC TGGACTTCAG 
AAGATTTTTG AGGATCCAGC AGACAGTGAT TTGCAGAGGT TCAGGTCTCC AACAATTGAA 
AACCCAGAAA GCTATTTCAG GGGGAAAACG CTACTGCTTG TCCAGCAGGC ATCCACACAG 
AGTATGTCCT TTTCCGACAA AGACGAAAGA GAGAATGTCT TGCCCAATGG GCGTAGTGTG 
TCTTTAATGG ATCTGCAAGA CTCCAATGTT AGTCACACTG ATCGTTCCTC AGTTATCCAA 
GAACCTCCCT TACGTTTAAC TGGCAGCCAG TTGTCTATTG CACATGTAGC TACCATTAAA 
CAGCTACGGG AAAACCATAG CATGCCTCAG AGTGCCCCAC AAGTTAGAAA GCCCTTACAT 
CCAGCATTGA GCCAGCAAAG CAGCCTGCAG CCTCTGTCCT TCCAAAATCC TGTGTATCAT 
CTGAACAACC CACCACCGAC AATGCCCAAA GCTTCTGTGG ACTCCAGCCT TGAAAATTTA 
AGCACTGCCA GCTCTAGAAG CCAGAGTAAT AGTGAAGAGT TCATTCTCAG TGGGCCCAGT 
AACAGTAGCA TGGAAGATTT CACAAAGAGG AGCACTCAGA GTGAGGACTT TACAAGAAGA 
CACACCATGC CTGAGAGACC TATTCCCATA GCACTGCCAA GGCAAAACAG CACTGGACAA 
GCCCAGATCC GTAAAACCGA CCAGGCTGCT CTTGGGGCTC GAGCAAAAGC CCCCCAGTCA 
TTACCACACA GTGCCTCACT GCGGAGCGCA GGGAGCATGT CTGGGATCTC CAATAATATG 
CTTGTTGAAC CAGTGCAGAA TGGAAATAGG TCCAGACAGC AATCTACATC ATCTCGTGAA 
AGCCCTGTAC CCAAACTAAG AGCAATTCAC CGTCAGCAGA CACAGCAGGT GCAGTCACCT 
GTGGACTCCG CTACAATGTC TCCAGTAGAG CGGACTGCAG CCTGGGTTCT TAATAACGGT 
CAATATGAAG ATGAAGAAGA AACTGTAGAC CAAAACAAGG ATGAATCTAA ACCTGCAGAG 
AAGTATGAAC AAGAGATTGC AAAGCTGAAA GAACGATTGA AAATTTCAAG TCGGCGCCTG 
GAGGAATATG AACGGCGTCT TTTGGTGCAA GAGCAGCAAA TGCAGAAGCT CTTAATGGAG 
TACAAATCTC GACTGGAGGA TAGCGAGGAG AGGTTGCGCC GTCAGCAGGA AGAGAAGGAC 
AGCCAAATGA AAAGTGTCAT CAGCAGGTTA ATAGCTGTAG AAGAGGAGCT GAAAAAAGAC 
CATGCTGAGA TGCAGGCTGT CATTGATGCA AAGCAAAAAA TTATTGATGC ACAGGAGAAG 
AGGATCATAT CACTGGATGC AGCCAACACG CGGCTAATGA GTGCCCTCAC ACAGGTCAAG 
GAACGCTATA ACTCCCAGGT GCGCAATGGA ATCTCTCCGA CCAACCCTAC CAAGCTGTCA 
ATCACTGAAA ATGGAGAATT TAAGAACAGC AGTTGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002937406.1
CDS	659..4477
Translation	MLMMDLSPGGDSVTSGHDFFQDRDSLPEDLDSPGGVGGILDRILMESVCQQQGWVRVYDVKGPPTHRLSCGQSPYTESTTWERKYCILTESQLVLLNRDDEMPGETAQDPPPESSKSRSLRRTVSVPSEGQFPDYPPEGAAKLEVPAERSPRRRSISGTSTSDKTNAMDPANTSPFKVSGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRPAESDRSRGLPKLKESRSHESLLSPGSAVEALDLGTEDKILVKPLHSSILGQDFCFEVTYSSGSKCFSCSSAAERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEYFEFYGLPPLHNITVHIYKDVEKKKKKDKNNYVGLVNIPMISVTGRQFVEKWYPVSTPTANKGKTVGPSIRIKSRYQTITILPMEQYKEFAEFITINYTMLCSVLEPVISVRNKEEMACALVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTLATKAIEEYLKLVGQKYLHDALGEFIKALYESDENCEVDPSKCSSSELSDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCINRGKQDISERLISASLFLRFLCPAIMSPSLFGLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLQEISNPDTISNTPGFEGYIDLGRELSVLHSLLWEVVSQLDKATVEKLGPLPRILADITKSLTNPTPIQQQLRRFSEHSSTPNVSGSLSSGLQKIFEDPADSDLQRFRSPTIENPESYFRGKTLLLVQQASTQSMSFSDKDERENVLPNGRSVSLMDLQDSNVSHTDRSSVIQEPPLRLTGSQLSIAHVATIKQLRENHSMPQSAPQVRKPLHPALSQQSSLQPLSFQNPVYHLNNPPPTMPKASVDSSLENLSTASSRSQSNSEEFILSGPSNSSMEDFTKRSTQSEDFTRRHTMPERPIPIALPRQNSTGQAQIRKTDQAALGARAKAPQSLPHSASLRSAGSMSGISNNMLVEPVQNGNRSRQQSTSSRESPVPKLRAIHRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEDEEETVDQNKDESKPAEKYEQEIAKLKERLKISSRRLEEYERRLLVQEQQMQKLLMEYKSRLEDSEERLRRQQEEKDSQMKSVISRLIAVEEELKKDHAEMQAVIDAKQKIIDAQEKRIISLDAANTRLMSALTQVKERYNSQVRNGISPTNPTKLSITENGEFKNSSC

Target ORF information:

RefSeq Version	XM_002937360.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis RAS protein activator like 2 (rasal2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002937360.4

ORF Insert Sequence:

ATGCTCATGA TGGATTTATC CCCCGGCGGG GATTCGGTAA CATCCGGACA TGACTTCTTC 
CAGGACAGAG ATTCCCTCCC AGAGGATTTG GACTCTCCCG GGGGAGTGGG GGGGATTCTG 
GACAGGATCC TAATGGAGTC TGTTTGCCAG CAGCAGGGAT GGGTGCGAGT ATACGATGTG 
AAAGGCCCGC CTACACACAG GCTGTCGTGT GGTCAGTCGC CCTACACTGA AAGCACAACA 
TGGGAGCGCA AGTACTGTAT CCTAACAGAA AGCCAACTGG TGCTCCTTAA CCGTGACGAT 
GAGATGCCTG GGGAAACTGC ACAGGACCCA CCTCCAGAAT CCAGCAAAAG CAGAAGCCTG 
CGTAGGACTG TGAGTGTCCC GTCAGAAGGG CAGTTTCCAG ACTACCCCCC GGAGGGTGCT 
GCCAAGCTGG AGGTCCCAGC TGAGAGGTCA CCCCGAAGGA GGAGTATCTC AGGGACTAGC 
ACGTCAGACA AAACCAATGC TATGGATCCT GCAAATACCT CCCCCTTTAA AGTCTCAGGC 
TTCTTTAGTA AACGGTTAAA AGGCTCTATT AAGAGAACGA AGAGTCAATC AAAGCTGGAC 
CGGAATACAA GTTTTCGCCT GCCTTCTCTT CGTCCTGCTG AGAGTGACCG GTCACGTGGG 
TTGCCTAAAC TAAAAGAATC TCGTTCCCAT GAATCTTTGC TGAGCCCTGG TAGTGCAGTT 
GAGGCTCTGG ATTTGGGAAC TGAAGATAAA ATCCTAGTAA AACCGCTCCA CAGCAGCATA 
TTGGGACAAG ATTTCTGCTT TGAGGTCACC TATTCCAGTG GCAGTAAATG TTTCAGCTGC 
TCATCTGCAG CAGAGAGAGA TAAGTGGATG GAAAATCTTC GTCGAACTGT TCAACCAAAC 
AAGGATAACT GTCGCCGAGC AGAGAATGTA CTACGGCTGT GGATCATTGA AGCCAAGGAC 
CTGGCTCCCA AAAAGAAGTA CTTCTGTGAA CTTTGTCTGG ACGATACTCT CTTTGCTCGA 
ACCACAAGCA AGACGAAAGC GGACAATATT TTTTGGGGGG AATATTTTGA GTTTTATGGT 
TTACCACCCC TGCACAACAT CACAGTTCAT ATTTACAAGG ATGTTGAAAA GAAGAAAAAA 
AAAGATAAAA ACAATTATGT TGGTTTAGTG AATATTCCTA TGATTAGTGT AACTGGGAGG 
CAGTTTGTGG AGAAATGGTA CCCAGTTAGC ACCCCCACTG CAAACAAAGG CAAGACTGTT 
GGTCCTTCCA TACGTATAAA GTCCCGTTAC CAAACTATTA CCATTTTACC CATGGAGCAA 
TATAAGGAAT TTGCAGAATT TATCACAATT AATTACACTA TGCTATGCTC AGTCCTGGAA 
CCAGTCATTA GTGTCAGAAA TAAGGAAGAG ATGGCATGTG CTTTAGTGCA CATCCTGCAG 
AGTACTGGCA GAGCCAAGGA CTTTCTAACT GATCTGGTGA TGTCAGAGGT AGATCGTTGT 
GGGGAGCACG ATGTCTTGAT CTTTAGGGAA AATACACTTG CCACTAAAGC CATTGAGGAG 
TATCTAAAGC TGGTTGGACA GAAGTACCTT CATGATGCTC TTGGGGAGTT TATCAAGGCA 
CTGTATGAGT CGGATGAGAA CTGTGAGGTG GATCCCAGCA AATGTTCCTC AAGTGAGCTG 
TCCGACCACC AGAGCAACTT AAAAATGTGC TGCGAGCTGG CCTTCTGCAA GATAATCAAT 
TCATATTGTG TTTTTCCCAG AGAGCTGAAG GAAGTGTTTG CTTCTTGGAA GCAGCAATGC 
ATAAATCGGG GAAAGCAGGA TATCAGCGAA CGACTGATCA GCGCATCACT CTTCCTTCGC 
TTTCTTTGTC CTGCAATTAT GTCTCCCAGC CTCTTTGGGC TAATGCAGGA GTATCCTGAT 
GATCGCACCT CCAGAACTCT CACACTCATT GCCAAGGTCA TCCAGAATCT TGCCAACTTT 
GCCAAGTTTG GTAATAAAGA AGAATACATG GCCTTTATGA ATGACTTTTT GGAGCATGAG 
TGGGGTGGAA TGAAACGATT CCTACAAGAG ATCTCCAATC CAGACACCAT TTCAAACACC 
CCCGGATTTG AGGGTTACAT AGACCTTGGC CGAGAGCTCT CCGTACTGCA CAGTCTTTTG 
TGGGAGGTGG TGTCACAGCT AGATAAGGCG ACCGTTGAGA AATTGGGACC CCTTCCACGC 
ATTCTTGCTG ACATCACCAA GTCACTGACC AACCCTACTC CAATCCAACA GCAGCTAAGA 
CGTTTCAGTG AACATAGCTC CACACCCAAT GTAAGTGGCA GCCTTTCATC TGGACTTCAG 
AAGATTTTTG AGGATCCAGC AGACAGTGAT TTGCAGAGGT TCAGGTCTCC AACAATTGAA 
AACCCAGAAA GCTATTTCAG GGGGAAAACG CTACTGCTTG TCCAGCAGGC ATCCACACAG 
AGTATGTCCT TTTCCGACAA AGACGAAAGA GAGAATGTCT TGCCCAATGG GCGTAGTGTG 
TCTTTAATGG ATCTGCAAGA CTCCAATGTT AGTCACACTG ATCGTTCCTC AGTTATCCAA 
GAACCTCCCT TACGTTTAAC TGGCAGCCAG TTGTCTATTG CACATGTAGC TACCATTAAA 
CAGCTACGGG AAAACCATAG CATGCCTCAG AGTGCCCCAC AAGTTAGAAA GCCCTTACAT 
CCAGCATTGA GCCAGCAAAG CAGCCTGCAG CCTCTGTCCT TCCAAAATCC TGTGTATCAT 
CTGAACAACC CACCACCGAC AATGCCCAAA GCTTCTGTGG ACTCCAGCCT TGAAAATTTA 
AGCACTGCCA GCTCTAGAAG CCAGAGTAAT AGTGAAGAGT TCATTCTCAG TGGGCCCAGT 
AACAGTAGCA TGGAAGATTT CACAAAGAGG AGCACTCAGA GTGAGGACTT TACAAGAAGA 
CACACCATGC CTGAGAGACC TATTCCCATA GCACTGCCAA GGCAAAACAG CACTGGACAA 
GCCCAGATCC GTAAAACCGA CCAGGCTGCT CTTGGGGCTC GAGCAAAAGC CCCCCAGTCA 
TTACCACACA GTGCCTCACT GCGGAGCGCA GGGAGCATGT CTGGGATCTC CAATAATATG 
CTTGTTGAAC CAGTGCAGAA TGGAAATAGG TCCAGACAGC AATCTACATC ATCTCGTGAA 
AGCCCTGTAC CCAAACTAAG AGCAATTCAC CGTCAGCAGA CACAGCAGGT GCAGTCACCT 
GTGGACTCCG CTACAATGTC TCCAGTAGAG CGGACTGCAG CCTGGGTTCT TAATAACGGT 
CAATATGAAG ATGAAGAAGA AACTGTAGAC CAAAACAAGG ATGAATCTAA ACCTGCAGAG 
AAGTATGAAC AAGAGATTGC AAAGCTGAAA GAACGATTGA AAATTTCAAG TCGGCGCCTG 
GAGGAATATG AACGGCGTCT TTTGGTGCAA GAGCAGCAAA TGCAGAAGCT CTTAATGGAG 
TACAAATCTC GACTGGAGGA TAGCGAGGAG AGGTTGCGCC GTCAGCAGGA AGAGAAGGAC 
AGCCAAATGA AAAGTGTCAT CAGCAGGTTA ATAGCTGTAG AAGAGGAGCT GAAAAAAGAC 
CATGCTGAGA TGCAGGCTGT CATTGATGCA AAGCAAAAAA TTATTGATGC ACAGGAGAAG 
AGGATCATAT CACTGGATGC AGCCAACACG CGGCTAATGA GTGCCCTCAC ACAGGTCAAG 
GAACGCTATA ACTCCCAGGT GCGCAATGGA ATCTCTCCGA CCAACCCTAC CAAGCTGTCA 
ATCACTGAAA ATGGAGAATT TAAGAACAGC AGTTGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rasal2 ( XM_004914031.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004914088.1 Xenopus tropicalis RAS protein activator like 2 (rasal2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa09555
Clone ID Related Accession (Same CDS sequence)	XM_004914031.3
Accession Version	XM_004914031.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3432bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ras GTPase-activating protein nGAP isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004914031.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTAGCA CATGCGATAA AGAGGTCCCA GCTGAGAGGT CACCCCGAAG GAGGAGTATC 
TCAGGGACTA GCACGTCAGA CAAAACCAAT GCTATGGATC CTGCAAATAC CTCCCCCTTT 
AAAGTCTCAG GCTTCTTTAG TAAACGGTTA AAAGGCTCTA TTAAGAGAAC GAAGAGTCAA 
TCAAAGCTGG ACCGGAATAC AAGTTTTCGC CTGCCTTCTC TTCGTCCTGC TGAGAGTGAC 
CGGTCACGTG GGTTGCCTAA ACTAAAAGAA TCTCGTTCCC ATGAATCTTT GCTGAGCCCT 
GGTAGTGCAG TTGAGGCTCT GGATTTGGGA ACTGAAGATA AAATCCTAGT AAAACCGCTC 
CACAGCAGCA TATTGGGACA AGATTTCTGC TTTGAGGTCA CCTATTCCAG TGGCAGTAAA 
TGTTTCAGCT GCTCATCTGC AGCAGAGAGA GATAAGTGGA TGGAAAATCT TCGTCGAACT 
GTTCAACCAA ACAAGGATAA CTGTCGCCGA GCAGAGAATG TACTACGGCT GTGGATCATT 
GAAGCCAAGG ACCTGGCTCC CAAAAAGAAG TACTTCTGTG AACTTTGTCT GGACGATACT 
CTCTTTGCTC GAACCACAAG CAAGACGAAA GCGGACAATA TTTTTTGGGG GGAATATTTT 
GAGTTTTATG GTTTACCACC CCTGCACAAC ATCACAGTTC ATATTTACAA GGATGTTGAA 
AAGAAGAAAA AAAAAGATAA AAACAATTAT GTTGGTTTAG TGAATATTCC TATGATTAGT 
GTAACTGGGA GGCAGTTTGT GGAGAAATGG TACCCAGTTA GCACCCCCAC TGCAAACAAA 
GGCAAGACTG TTGGTCCTTC CATACGTATA AAGTCCCGTT ACCAAACTAT TACCATTTTA 
CCCATGGAGC AATATAAGGA ATTTGCAGAA TTTATCACAA TTAATTACAC TATGCTATGC 
TCAGTCCTGG AACCAGTCAT TAGTGTCAGA AATAAGGAAG AGATGGCATG TGCTTTAGTG 
CACATCCTGC AGAGTACTGG CAGAGCCAAG GACTTTCTAA CTGATCTGGT GATGTCAGAG 
GTAGATCGTT GTGGGGAGCA CGATGTCTTG ATCTTTAGGG AAAATACACT TGCCACTAAA 
GCCATTGAGG AGTATCTAAA GCTGGTTGGA CAGAAGTACC TTCATGATGC TCTTGGGGAG 
TTTATCAAGG CACTGTATGA GTCGGATGAG AACTGTGAGG TGGATCCCAG CAAATGTTCC 
TCAAGTGAGC TGTCCGACCA CCAGAGCAAC TTAAAAATGT GCTGCGAGCT GGCCTTCTGC 
AAGATAATCA ATTCATATTG TGTTTTTCCC AGAGAGCTGA AGGAAGTGTT TGCTTCTTGG 
AAGCAGCAAT GCATAAATCG GGGAAAGCAG GATATCAGCG AACGACTGAT CAGCGCATCA 
CTCTTCCTTC GCTTTCTTTG TCCTGCAATT ATGTCTCCCA GCCTCTTTGG GCTAATGCAG 
GAGTATCCTG ATGATCGCAC CTCCAGAACT CTCACACTCA TTGCCAAGGT CATCCAGAAT 
CTTGCCAACT TTGCCAAGTT TGGTAATAAA GAAGAATACA TGGCCTTTAT GAATGACTTT 
TTGGAGCATG AGTGGGGTGG AATGAAACGA TTCCTACAAG AGATCTCCAA TCCAGACACC 
ATTTCAAACA CCCCCGGATT TGAGGGTTAC ATAGACCTTG GCCGAGAGCT CTCCGTACTG 
CACAGTCTTT TGTGGGAGGT GGTGTCACAG CTAGATAAGG GTGAAAATTC CTTCCTACAG 
GCGACCGTTG AGAAATTGGG ACCCCTTCCA CGCATTCTTG CTGACATCAC CAAGTCACTG 
ACCAACCCTA CTCCAATCCA ACAGCAGCTA AGACGTTTCA GTGAACATAG CTCCACACCC 
AATGTAAGTG GCAGCCTTTC ATCTGGACTT CAGAAGATTT TTGAGGATCC AGCAGACAGT 
GATTTGCAGA GGTTCAGGTC TCCAACAATT GAAAACCCAG AAAGCTATTT CAGGGGGAAA 
ACGCTACTGC TTGTCCAGCA GGCATCCACA CAGAGTATGT CCTTTTCCGA CAAAGACGAA 
AGAGAGAATG TCTTGCCCAA TGGGCGTAGT GTGTCTTTAA TGGATCTGCA AGACTCCAAT 
GTTAGTCACA CTGATCGTTC CTCAGTTATC CAAGAACCTC CCTTACGTTT AACTGGCAGC 
CAGTTGTCTA TTGCACATGT AGCTACCATT AAACAGCTAC GGGAAAACCA TAGCATGCCT 
CAGAGTGCCC CACAAGTTAG AAAGCCCTTA CATCCAGCAT TGAGCCAGCA AAGCAGCCTG 
CAGCCTCTGT CCTTCCAAAA TCCTGTGTAT CATCTGAACA ACCCACCACC GACAATGCCC 
AAAGCTTCTG TGGACTCCAG CCTTGAAAAT TTAAGCACTG CCAGCTCTAG AAGCCAGAGT 
AATAGTGAAG AGTTCATTCT CAGTGGGCCC AGTAACAGTA GCATGGAAGA TTTCACAAAG 
AGGAGCACTC AGAGTGAGGA CTTTACAAGA AGACACACCA TGCCTGAGAG ACCTATTCCC 
ATAGCACTGC CAAGGCAAAA CAGCACTGGA CAAGCCCAGA TCCGTAAAAC CGACCAGGCT 
GCTCTTGGGG CTCGAGCAAA AGCCCCCCAG TCATTACCAC ACAGTGCCTC ACTGCGGAGC 
GCAGGGAGCA TGTCTGGGAT CTCCAATAAT ATGCTTGTTG AACCAGTGCA GAATGGAAAT 
AGGTCCAGAC AGCAATCTAC ATCATCTCGT GAAAGCCCTG TACCCAAACT AAGAGCAATT 
CACCGTCAGC AGACACAGCA GGTGCAGTCA CCTGTGGACT CCGCTACAAT GTCTCCAGTA 
GAGCGGACTG CAGCCTGGGT TCTTAATAAC GGTCAATATG AAGATGAAGA AGAAACTGTA 
GACCAAAACA AGGATGAATC TAAACCTGCA GAGAAGTATG AACAAGAGAT TGCAAAGCTG 
AAAGAACGAT TGAAAATTTC AAGTCGGCGC CTGGAGGAAT ATGAACGGCG TCTTTTGGTG 
CAAGAGCAGC AAATGCAGAA GCTCTTAATG GAGTACAAAT CTCGACTGGA GGATAGCGAG 
GAGAGGTTGC GCCGTCAGCA GGAAGAGAAG GACAGCCAAA TGAAAAGTGT CATCAGCAGG 
TTAATAGCTG TAGAAGAGGA GCTGAAAAAA GACCATGCTG AGATGCAGGC TGTCATTGAT 
GCAAAGCAAA AAATTATTGA TGCACAGGAG AAGAGGATCA TATCACTGGA TGCAGCCAAC 
ACGCGGCTAA TGAGTGCCCT CACACAGGTC AAGGAACGCT ATAACTCCCA GGTGCGCAAT 
GGAATCTCTC CGACCAACCC TACCAAGCTG TCAATCACTG AAAATGGAGA ATTTAAGAAC 
AGCAGTTGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004914088.1
CDS	83..3514
Translation	MGSTCDKEVPAERSPRRRSISGTSTSDKTNAMDPANTSPFKVSGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRPAESDRSRGLPKLKESRSHESLLSPGSAVEALDLGTEDKILVKPLHSSILGQDFCFEVTYSSGSKCFSCSSAAERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEYFEFYGLPPLHNITVHIYKDVEKKKKKDKNNYVGLVNIPMISVTGRQFVEKWYPVSTPTANKGKTVGPSIRIKSRYQTITILPMEQYKEFAEFITINYTMLCSVLEPVISVRNKEEMACALVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTLATKAIEEYLKLVGQKYLHDALGEFIKALYESDENCEVDPSKCSSSELSDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCINRGKQDISERLISASLFLRFLCPAIMSPSLFGLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLQEISNPDTISNTPGFEGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVEKLGPLPRILADITKSLTNPTPIQQQLRRFSEHSSTPNVSGSLSSGLQKIFEDPADSDLQRFRSPTIENPESYFRGKTLLLVQQASTQSMSFSDKDERENVLPNGRSVSLMDLQDSNVSHTDRSSVIQEPPLRLTGSQLSIAHVATIKQLRENHSMPQSAPQVRKPLHPALSQQSSLQPLSFQNPVYHLNNPPPTMPKASVDSSLENLSTASSRSQSNSEEFILSGPSNSSMEDFTKRSTQSEDFTRRHTMPERPIPIALPRQNSTGQAQIRKTDQAALGARAKAPQSLPHSASLRSAGSMSGISNNMLVEPVQNGNRSRQQSTSSRESPVPKLRAIHRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEDEEETVDQNKDESKPAEKYEQEIAKLKERLKISSRRLEEYERRLLVQEQQMQKLLMEYKSRLEDSEERLRRQQEEKDSQMKSVISRLIAVEEELKKDHAEMQAVIDAKQKIIDAQEKRIISLDAANTRLMSALTQVKERYNSQVRNGISPTNPTKLSITENGEFKNSSC

Target ORF information:

RefSeq Version	XM_004914031.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis RAS protein activator like 2 (rasal2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004914031.3

ORF Insert Sequence:

ATGGGTAGCA CATGCGATAA AGAGGTCCCA GCTGAGAGGT CACCCCGAAG GAGGAGTATC 
TCAGGGACTA GCACGTCAGA CAAAACCAAT GCTATGGATC CTGCAAATAC CTCCCCCTTT 
AAAGTCTCAG GCTTCTTTAG TAAACGGTTA AAAGGCTCTA TTAAGAGAAC GAAGAGTCAA 
TCAAAGCTGG ACCGGAATAC AAGTTTTCGC CTGCCTTCTC TTCGTCCTGC TGAGAGTGAC 
CGGTCACGTG GGTTGCCTAA ACTAAAAGAA TCTCGTTCCC ATGAATCTTT GCTGAGCCCT 
GGTAGTGCAG TTGAGGCTCT GGATTTGGGA ACTGAAGATA AAATCCTAGT AAAACCGCTC 
CACAGCAGCA TATTGGGACA AGATTTCTGC TTTGAGGTCA CCTATTCCAG TGGCAGTAAA 
TGTTTCAGCT GCTCATCTGC AGCAGAGAGA GATAAGTGGA TGGAAAATCT TCGTCGAACT 
GTTCAACCAA ACAAGGATAA CTGTCGCCGA GCAGAGAATG TACTACGGCT GTGGATCATT 
GAAGCCAAGG ACCTGGCTCC CAAAAAGAAG TACTTCTGTG AACTTTGTCT GGACGATACT 
CTCTTTGCTC GAACCACAAG CAAGACGAAA GCGGACAATA TTTTTTGGGG GGAATATTTT 
GAGTTTTATG GTTTACCACC CCTGCACAAC ATCACAGTTC ATATTTACAA GGATGTTGAA 
AAGAAGAAAA AAAAAGATAA AAACAATTAT GTTGGTTTAG TGAATATTCC TATGATTAGT 
GTAACTGGGA GGCAGTTTGT GGAGAAATGG TACCCAGTTA GCACCCCCAC TGCAAACAAA 
GGCAAGACTG TTGGTCCTTC CATACGTATA AAGTCCCGTT ACCAAACTAT TACCATTTTA 
CCCATGGAGC AATATAAGGA ATTTGCAGAA TTTATCACAA TTAATTACAC TATGCTATGC 
TCAGTCCTGG AACCAGTCAT TAGTGTCAGA AATAAGGAAG AGATGGCATG TGCTTTAGTG 
CACATCCTGC AGAGTACTGG CAGAGCCAAG GACTTTCTAA CTGATCTGGT GATGTCAGAG 
GTAGATCGTT GTGGGGAGCA CGATGTCTTG ATCTTTAGGG AAAATACACT TGCCACTAAA 
GCCATTGAGG AGTATCTAAA GCTGGTTGGA CAGAAGTACC TTCATGATGC TCTTGGGGAG 
TTTATCAAGG CACTGTATGA GTCGGATGAG AACTGTGAGG TGGATCCCAG CAAATGTTCC 
TCAAGTGAGC TGTCCGACCA CCAGAGCAAC TTAAAAATGT GCTGCGAGCT GGCCTTCTGC 
AAGATAATCA ATTCATATTG TGTTTTTCCC AGAGAGCTGA AGGAAGTGTT TGCTTCTTGG 
AAGCAGCAAT GCATAAATCG GGGAAAGCAG GATATCAGCG AACGACTGAT CAGCGCATCA 
CTCTTCCTTC GCTTTCTTTG TCCTGCAATT ATGTCTCCCA GCCTCTTTGG GCTAATGCAG 
GAGTATCCTG ATGATCGCAC CTCCAGAACT CTCACACTCA TTGCCAAGGT CATCCAGAAT 
CTTGCCAACT TTGCCAAGTT TGGTAATAAA GAAGAATACA TGGCCTTTAT GAATGACTTT 
TTGGAGCATG AGTGGGGTGG AATGAAACGA TTCCTACAAG AGATCTCCAA TCCAGACACC 
ATTTCAAACA CCCCCGGATT TGAGGGTTAC ATAGACCTTG GCCGAGAGCT CTCCGTACTG 
CACAGTCTTT TGTGGGAGGT GGTGTCACAG CTAGATAAGG GTGAAAATTC CTTCCTACAG 
GCGACCGTTG AGAAATTGGG ACCCCTTCCA CGCATTCTTG CTGACATCAC CAAGTCACTG 
ACCAACCCTA CTCCAATCCA ACAGCAGCTA AGACGTTTCA GTGAACATAG CTCCACACCC 
AATGTAAGTG GCAGCCTTTC ATCTGGACTT CAGAAGATTT TTGAGGATCC AGCAGACAGT 
GATTTGCAGA GGTTCAGGTC TCCAACAATT GAAAACCCAG AAAGCTATTT CAGGGGGAAA 
ACGCTACTGC TTGTCCAGCA GGCATCCACA CAGAGTATGT CCTTTTCCGA CAAAGACGAA 
AGAGAGAATG TCTTGCCCAA TGGGCGTAGT GTGTCTTTAA TGGATCTGCA AGACTCCAAT 
GTTAGTCACA CTGATCGTTC CTCAGTTATC CAAGAACCTC CCTTACGTTT AACTGGCAGC 
CAGTTGTCTA TTGCACATGT AGCTACCATT AAACAGCTAC GGGAAAACCA TAGCATGCCT 
CAGAGTGCCC CACAAGTTAG AAAGCCCTTA CATCCAGCAT TGAGCCAGCA AAGCAGCCTG 
CAGCCTCTGT CCTTCCAAAA TCCTGTGTAT CATCTGAACA ACCCACCACC GACAATGCCC 
AAAGCTTCTG TGGACTCCAG CCTTGAAAAT TTAAGCACTG CCAGCTCTAG AAGCCAGAGT 
AATAGTGAAG AGTTCATTCT CAGTGGGCCC AGTAACAGTA GCATGGAAGA TTTCACAAAG 
AGGAGCACTC AGAGTGAGGA CTTTACAAGA AGACACACCA TGCCTGAGAG ACCTATTCCC 
ATAGCACTGC CAAGGCAAAA CAGCACTGGA CAAGCCCAGA TCCGTAAAAC CGACCAGGCT 
GCTCTTGGGG CTCGAGCAAA AGCCCCCCAG TCATTACCAC ACAGTGCCTC ACTGCGGAGC 
GCAGGGAGCA TGTCTGGGAT CTCCAATAAT ATGCTTGTTG AACCAGTGCA GAATGGAAAT 
AGGTCCAGAC AGCAATCTAC ATCATCTCGT GAAAGCCCTG TACCCAAACT AAGAGCAATT 
CACCGTCAGC AGACACAGCA GGTGCAGTCA CCTGTGGACT CCGCTACAAT GTCTCCAGTA 
GAGCGGACTG CAGCCTGGGT TCTTAATAAC GGTCAATATG AAGATGAAGA AGAAACTGTA 
GACCAAAACA AGGATGAATC TAAACCTGCA GAGAAGTATG AACAAGAGAT TGCAAAGCTG 
AAAGAACGAT TGAAAATTTC AAGTCGGCGC CTGGAGGAAT ATGAACGGCG TCTTTTGGTG 
CAAGAGCAGC AAATGCAGAA GCTCTTAATG GAGTACAAAT CTCGACTGGA GGATAGCGAG 
GAGAGGTTGC GCCGTCAGCA GGAAGAGAAG GACAGCCAAA TGAAAAGTGT CATCAGCAGG 
TTAATAGCTG TAGAAGAGGA GCTGAAAAAA GACCATGCTG AGATGCAGGC TGTCATTGAT 
GCAAAGCAAA AAATTATTGA TGCACAGGAG AAGAGGATCA TATCACTGGA TGCAGCCAAC 
ACGCGGCTAA TGAGTGCCCT CACACAGGTC AAGGAACGCT ATAACTCCCA GGTGCGCAAT 
GGAATCTCTC CGACCAACCC TACCAAGCTG TCAATCACTG AAAATGGAGA ATTTAAGAAC 
AGCAGTTGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rasal2 ( XM_004914032.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004914089.1 Xenopus tropicalis RAS protein activator like 2 (rasal2), transcript variant X5, mRNA.

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CloneID	OXa09556
Clone ID Related Accession (Same CDS sequence)	XM_004914032.3
Accession Version	XM_004914032.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ras GTPase-activating protein nGAP isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004914032.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCTCA CAGAGGTCCC AGCTGAGAGG TCACCCCGAA GGAGGAGTAT CTCAGGGACT 
AGCACGTCAG ACAAAACCAA TGCTATGGAT CCTGCAAATA CCTCCCCCTT TAAAGTCTCA 
GGCTTCTTTA GTAAACGGTT AAAAGGCTCT ATTAAGAGAA CGAAGAGTCA ATCAAAGCTG 
GACCGGAATA CAAGTTTTCG CCTGCCTTCT CTTCGTCCTG CTGAGAGTGA CCGGTCACGT 
GGGTTGCCTA AACTAAAAGA ATCTCGTTCC CATGAATCTT TGCTGAGCCC TGGTAGTGCA 
GTTGAGGCTC TGGATTTGGG AACTGAAGAT AAAATCCTAG TAAAACCGCT CCACAGCAGC 
ATATTGGGAC AAGATTTCTG CTTTGAGGTC ACCTATTCCA GTGGCAGTAA ATGTTTCAGC 
TGCTCATCTG CAGCAGAGAG AGATAAGTGG ATGGAAAATC TTCGTCGAAC TGTTCAACCA 
AACAAGGATA ACTGTCGCCG AGCAGAGAAT GTACTACGGC TGTGGATCAT TGAAGCCAAG 
GACCTGGCTC CCAAAAAGAA GTACTTCTGT GAACTTTGTC TGGACGATAC TCTCTTTGCT 
CGAACCACAA GCAAGACGAA AGCGGACAAT ATTTTTTGGG GGGAATATTT TGAGTTTTAT 
GGTTTACCAC CCCTGCACAA CATCACAGTT CATATTTACA AGGATGTTGA AAAGAAGAAA 
AAAAAAGATA AAAACAATTA TGTTGGTTTA GTGAATATTC CTATGATTAG TGTAACTGGG 
AGGCAGTTTG TGGAGAAATG GTACCCAGTT AGCACCCCCA CTGCAAACAA AGGCAAGACT 
GTTGGTCCTT CCATACGTAT AAAGTCCCGT TACCAAACTA TTACCATTTT ACCCATGGAG 
CAATATAAGG AATTTGCAGA ATTTATCACA ATTAATTACA CTATGCTATG CTCAGTCCTG 
GAACCAGTCA TTAGTGTCAG AAATAAGGAA GAGATGGCAT GTGCTTTAGT GCACATCCTG 
CAGAGTACTG GCAGAGCCAA GGACTTTCTA ACTGATCTGG TGATGTCAGA GGTAGATCGT 
TGTGGGGAGC ACGATGTCTT GATCTTTAGG GAAAATACAC TTGCCACTAA AGCCATTGAG 
GAGTATCTAA AGCTGGTTGG ACAGAAGTAC CTTCATGATG CTCTTGGGGA GTTTATCAAG 
GCACTGTATG AGTCGGATGA GAACTGTGAG GTGGATCCCA GCAAATGTTC CTCAAGTGAG 
CTGTCCGACC ACCAGAGCAA CTTAAAAATG TGCTGCGAGC TGGCCTTCTG CAAGATAATC 
AATTCATATT GTGTTTTTCC CAGAGAGCTG AAGGAAGTGT TTGCTTCTTG GAAGCAGCAA 
TGCATAAATC GGGGAAAGCA GGATATCAGC GAACGACTGA TCAGCGCATC ACTCTTCCTT 
CGCTTTCTTT GTCCTGCAAT TATGTCTCCC AGCCTCTTTG GGCTAATGCA GGAGTATCCT 
GATGATCGCA CCTCCAGAAC TCTCACACTC ATTGCCAAGG TCATCCAGAA TCTTGCCAAC 
TTTGCCAAGT TTGGTAATAA AGAAGAATAC ATGGCCTTTA TGAATGACTT TTTGGAGCAT 
GAGTGGGGTG GAATGAAACG ATTCCTACAA GAGATCTCCA ATCCAGACAC CATTTCAAAC 
ACCCCCGGAT TTGAGGGTTA CATAGACCTT GGCCGAGAGC TCTCCGTACT GCACAGTCTT 
TTGTGGGAGG TGGTGTCACA GCTAGATAAG GGTGAAAATT CCTTCCTACA GGCGACCGTT 
GAGAAATTGG GACCCCTTCC ACGCATTCTT GCTGACATCA CCAAGTCACT GACCAACCCT 
ACTCCAATCC AACAGCAGCT AAGACGTTTC AGTGAACATA GCTCCACACC CAATGTAAGT 
GGCAGCCTTT CATCTGGACT TCAGAAGATT TTTGAGGATC CAGCAGACAG TGATTTGCAG 
AGGTTCAGGT CTCCAACAAT TGAAAACCCA GAAAGCTATT TCAGGGGGAA AACGCTACTG 
CTTGTCCAGC AGGCATCCAC ACAGAGTATG TCCTTTTCCG ACAAAGACGA AAGAGAGAAT 
GTCTTGCCCA ATGGGCGTAG TGTGTCTTTA ATGGATCTGC AAGACTCCAA TGTTAGTCAC 
ACTGATCGTT CCTCAGTTAT CCAAGAACCT CCCTTACGTT TAACTGGCAG CCAGTTGTCT 
ATTGCACATG TAGCTACCAT TAAACAGCTA CGGGAAAACC ATAGCATGCC TCAGAGTGCC 
CCACAAGTTA GAAAGCCCTT ACATCCAGCA TTGAGCCAGC AAAGCAGCCT GCAGCCTCTG 
TCCTTCCAAA ATCCTGTGTA TCATCTGAAC AACCCACCAC CGACAATGCC CAAAGCTTCT 
GTGGACTCCA GCCTTGAAAA TTTAAGCACT GCCAGCTCTA GAAGCCAGAG TAATAGTGAA 
GAGTTCATTC TCAGTGGGCC CAGTAACAGT AGCATGGAAG ATTTCACAAA GAGGAGCACT 
CAGAGTGAGG ACTTTACAAG AAGACACACC ATGCCTGAGA GACCTATTCC CATAGCACTG 
CCAAGGCAAA ACAGCACTGG ACAAGCCCAG ATCCGTAAAA CCGACCAGGC TGCTCTTGGG 
GCTCGAGCAA AAGCCCCCCA GTCATTACCA CACAGTGCCT CACTGCGGAG CGCAGGGAGC 
ATGTCTGGGA TCTCCAATAA TATGCTTGTT GAACCAGTGC AGAATGGAAA TAGGTCCAGA 
CAGCAATCTA CATCATCTCG TGAAAGCCCT GTACCCAAAC TAAGAGCAAT TCACCGTCAG 
CAGACACAGC AGGTGCAGTC ACCTGTGGAC TCCGCTACAA TGTCTCCAGT AGAGCGGACT 
GCAGCCTGGG TTCTTAATAA CGGTCAATAT GAAGATGAAG AAGAAACTGT AGACCAAAAC 
AAGGATGAAT CTAAACCTGC AGAGAAGTAT GAACAAGAGA TTGCAAAGCT GAAAGAACGA 
TTGAAAATTT CAAGTCGGCG CCTGGAGGAA TATGAACGGC GTCTTTTGGT GCAAGAGCAG 
CAAATGCAGA AGCTCTTAAT GGAGTACAAA TCTCGACTGG AGGATAGCGA GGAGAGGTTG 
CGCCGTCAGC AGGAAGAGAA GGACAGCCAA ATGAAAAGTG TCATCAGCAG GTTAATAGCT 
GTAGAAGAGG AGCTGAAAAA AGACCATGCT GAGATGCAGG CTGTCATTGA TGCAAAGCAA 
AAAATTATTG ATGCACAGGA GAAGAGGATC ATATCACTGG ATGCAGCCAA CACGCGGCTA 
ATGAGTGCCC TCACACAGGT CAAGGAACGC TATAACTCCC AGGTGCGCAA TGGAATCTCT 
CCGACCAACC CTACCAAGCT GTCAATCACT GAAAATGGAG AATTTAAGAA CAGCAGTTGT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004914089.1
CDS	106..3528
Translation	MQLTEVPAERSPRRRSISGTSTSDKTNAMDPANTSPFKVSGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRPAESDRSRGLPKLKESRSHESLLSPGSAVEALDLGTEDKILVKPLHSSILGQDFCFEVTYSSGSKCFSCSSAAERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEYFEFYGLPPLHNITVHIYKDVEKKKKKDKNNYVGLVNIPMISVTGRQFVEKWYPVSTPTANKGKTVGPSIRIKSRYQTITILPMEQYKEFAEFITINYTMLCSVLEPVISVRNKEEMACALVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTLATKAIEEYLKLVGQKYLHDALGEFIKALYESDENCEVDPSKCSSSELSDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCINRGKQDISERLISASLFLRFLCPAIMSPSLFGLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLQEISNPDTISNTPGFEGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVEKLGPLPRILADITKSLTNPTPIQQQLRRFSEHSSTPNVSGSLSSGLQKIFEDPADSDLQRFRSPTIENPESYFRGKTLLLVQQASTQSMSFSDKDERENVLPNGRSVSLMDLQDSNVSHTDRSSVIQEPPLRLTGSQLSIAHVATIKQLRENHSMPQSAPQVRKPLHPALSQQSSLQPLSFQNPVYHLNNPPPTMPKASVDSSLENLSTASSRSQSNSEEFILSGPSNSSMEDFTKRSTQSEDFTRRHTMPERPIPIALPRQNSTGQAQIRKTDQAALGARAKAPQSLPHSASLRSAGSMSGISNNMLVEPVQNGNRSRQQSTSSRESPVPKLRAIHRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEDEEETVDQNKDESKPAEKYEQEIAKLKERLKISSRRLEEYERRLLVQEQQMQKLLMEYKSRLEDSEERLRRQQEEKDSQMKSVISRLIAVEEELKKDHAEMQAVIDAKQKIIDAQEKRIISLDAANTRLMSALTQVKERYNSQVRNGISPTNPTKLSITENGEFKNSSC

Target ORF information:

RefSeq Version	XM_004914032.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis RAS protein activator like 2 (rasal2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004914032.3

ORF Insert Sequence:

ATGCAGCTCA CAGAGGTCCC AGCTGAGAGG TCACCCCGAA GGAGGAGTAT CTCAGGGACT 
AGCACGTCAG ACAAAACCAA TGCTATGGAT CCTGCAAATA CCTCCCCCTT TAAAGTCTCA 
GGCTTCTTTA GTAAACGGTT AAAAGGCTCT ATTAAGAGAA CGAAGAGTCA ATCAAAGCTG 
GACCGGAATA CAAGTTTTCG CCTGCCTTCT CTTCGTCCTG CTGAGAGTGA CCGGTCACGT 
GGGTTGCCTA AACTAAAAGA ATCTCGTTCC CATGAATCTT TGCTGAGCCC TGGTAGTGCA 
GTTGAGGCTC TGGATTTGGG AACTGAAGAT AAAATCCTAG TAAAACCGCT CCACAGCAGC 
ATATTGGGAC AAGATTTCTG CTTTGAGGTC ACCTATTCCA GTGGCAGTAA ATGTTTCAGC 
TGCTCATCTG CAGCAGAGAG AGATAAGTGG ATGGAAAATC TTCGTCGAAC TGTTCAACCA 
AACAAGGATA ACTGTCGCCG AGCAGAGAAT GTACTACGGC TGTGGATCAT TGAAGCCAAG 
GACCTGGCTC CCAAAAAGAA GTACTTCTGT GAACTTTGTC TGGACGATAC TCTCTTTGCT 
CGAACCACAA GCAAGACGAA AGCGGACAAT ATTTTTTGGG GGGAATATTT TGAGTTTTAT 
GGTTTACCAC CCCTGCACAA CATCACAGTT CATATTTACA AGGATGTTGA AAAGAAGAAA 
AAAAAAGATA AAAACAATTA TGTTGGTTTA GTGAATATTC CTATGATTAG TGTAACTGGG 
AGGCAGTTTG TGGAGAAATG GTACCCAGTT AGCACCCCCA CTGCAAACAA AGGCAAGACT 
GTTGGTCCTT CCATACGTAT AAAGTCCCGT TACCAAACTA TTACCATTTT ACCCATGGAG 
CAATATAAGG AATTTGCAGA ATTTATCACA ATTAATTACA CTATGCTATG CTCAGTCCTG 
GAACCAGTCA TTAGTGTCAG AAATAAGGAA GAGATGGCAT GTGCTTTAGT GCACATCCTG 
CAGAGTACTG GCAGAGCCAA GGACTTTCTA ACTGATCTGG TGATGTCAGA GGTAGATCGT 
TGTGGGGAGC ACGATGTCTT GATCTTTAGG GAAAATACAC TTGCCACTAA AGCCATTGAG 
GAGTATCTAA AGCTGGTTGG ACAGAAGTAC CTTCATGATG CTCTTGGGGA GTTTATCAAG 
GCACTGTATG AGTCGGATGA GAACTGTGAG GTGGATCCCA GCAAATGTTC CTCAAGTGAG 
CTGTCCGACC ACCAGAGCAA CTTAAAAATG TGCTGCGAGC TGGCCTTCTG CAAGATAATC 
AATTCATATT GTGTTTTTCC CAGAGAGCTG AAGGAAGTGT TTGCTTCTTG GAAGCAGCAA 
TGCATAAATC GGGGAAAGCA GGATATCAGC GAACGACTGA TCAGCGCATC ACTCTTCCTT 
CGCTTTCTTT GTCCTGCAAT TATGTCTCCC AGCCTCTTTG GGCTAATGCA GGAGTATCCT 
GATGATCGCA CCTCCAGAAC TCTCACACTC ATTGCCAAGG TCATCCAGAA TCTTGCCAAC 
TTTGCCAAGT TTGGTAATAA AGAAGAATAC ATGGCCTTTA TGAATGACTT TTTGGAGCAT 
GAGTGGGGTG GAATGAAACG ATTCCTACAA GAGATCTCCA ATCCAGACAC CATTTCAAAC 
ACCCCCGGAT TTGAGGGTTA CATAGACCTT GGCCGAGAGC TCTCCGTACT GCACAGTCTT 
TTGTGGGAGG TGGTGTCACA GCTAGATAAG GGTGAAAATT CCTTCCTACA GGCGACCGTT 
GAGAAATTGG GACCCCTTCC ACGCATTCTT GCTGACATCA CCAAGTCACT GACCAACCCT 
ACTCCAATCC AACAGCAGCT AAGACGTTTC AGTGAACATA GCTCCACACC CAATGTAAGT 
GGCAGCCTTT CATCTGGACT TCAGAAGATT TTTGAGGATC CAGCAGACAG TGATTTGCAG 
AGGTTCAGGT CTCCAACAAT TGAAAACCCA GAAAGCTATT TCAGGGGGAA AACGCTACTG 
CTTGTCCAGC AGGCATCCAC ACAGAGTATG TCCTTTTCCG ACAAAGACGA AAGAGAGAAT 
GTCTTGCCCA ATGGGCGTAG TGTGTCTTTA ATGGATCTGC AAGACTCCAA TGTTAGTCAC 
ACTGATCGTT CCTCAGTTAT CCAAGAACCT CCCTTACGTT TAACTGGCAG CCAGTTGTCT 
ATTGCACATG TAGCTACCAT TAAACAGCTA CGGGAAAACC ATAGCATGCC TCAGAGTGCC 
CCACAAGTTA GAAAGCCCTT ACATCCAGCA TTGAGCCAGC AAAGCAGCCT GCAGCCTCTG 
TCCTTCCAAA ATCCTGTGTA TCATCTGAAC AACCCACCAC CGACAATGCC CAAAGCTTCT 
GTGGACTCCA GCCTTGAAAA TTTAAGCACT GCCAGCTCTA GAAGCCAGAG TAATAGTGAA 
GAGTTCATTC TCAGTGGGCC CAGTAACAGT AGCATGGAAG ATTTCACAAA GAGGAGCACT 
CAGAGTGAGG ACTTTACAAG AAGACACACC ATGCCTGAGA GACCTATTCC CATAGCACTG 
CCAAGGCAAA ACAGCACTGG ACAAGCCCAG ATCCGTAAAA CCGACCAGGC TGCTCTTGGG 
GCTCGAGCAA AAGCCCCCCA GTCATTACCA CACAGTGCCT CACTGCGGAG CGCAGGGAGC 
ATGTCTGGGA TCTCCAATAA TATGCTTGTT GAACCAGTGC AGAATGGAAA TAGGTCCAGA 
CAGCAATCTA CATCATCTCG TGAAAGCCCT GTACCCAAAC TAAGAGCAAT TCACCGTCAG 
CAGACACAGC AGGTGCAGTC ACCTGTGGAC TCCGCTACAA TGTCTCCAGT AGAGCGGACT 
GCAGCCTGGG TTCTTAATAA CGGTCAATAT GAAGATGAAG AAGAAACTGT AGACCAAAAC 
AAGGATGAAT CTAAACCTGC AGAGAAGTAT GAACAAGAGA TTGCAAAGCT GAAAGAACGA 
TTGAAAATTT CAAGTCGGCG CCTGGAGGAA TATGAACGGC GTCTTTTGGT GCAAGAGCAG 
CAAATGCAGA AGCTCTTAAT GGAGTACAAA TCTCGACTGG AGGATAGCGA GGAGAGGTTG 
CGCCGTCAGC AGGAAGAGAA GGACAGCCAA ATGAAAAGTG TCATCAGCAG GTTAATAGCT 
GTAGAAGAGG AGCTGAAAAA AGACCATGCT GAGATGCAGG CTGTCATTGA TGCAAAGCAA 
AAAATTATTG ATGCACAGGA GAAGAGGATC ATATCACTGG ATGCAGCCAA CACGCGGCTA 
ATGAGTGCCC TCACACAGGT CAAGGAACGC TATAACTCCC AGGTGCGCAA TGGAATCTCT 
CCGACCAACC CTACCAAGCT GTCAATCACT GAAAATGGAG AATTTAAGAA CAGCAGTTGT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	rasal2
Entrez Gene ID	100379974
Full Name	RAS protein activator like 2
General protein information	Preferred Names RAS protein activator like 2 Names RAS protein activator like 2 ras GTPase-activating protein nGAP
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

Danio rerio (zebrafish)	rasal2	XP_001332688.5
Xenopus tropicalis (tropical clawed frog)	rasal2	XP_004914087.1
Homo sapiens (human)	RASAL2	NP_733793.2
Gallus gallus (chicken)	RASAL2	XP_004943302.1
Canis lupus familiaris (dog)	RASAL2	XP_005622535.1
Bos taurus (cattle)	RASAL2	XP_005217296.1
Pan troglodytes (chimpanzee)	RASAL2	XP_003949696.1
Mus musculus (house mouse)	Rasal2	NP_808312.3
	RASAL2	XP_002802086.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

rasal2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

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