RASAL2 cDNA ORF clone, Bos taurus(cattle)

The following RASAL2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RASAL2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa08809 XM_005217238.4
Latest version!
Bos taurus RAS protein activator like 2 (RASAL2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OBa08810 XM_005217239.4
Latest version!
Bos taurus RAS protein activator like 2 (RASAL2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OBa08811 XM_005217240.4
Latest version!
Bos taurus RAS protein activator like 2 (RASAL2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OBa08812 XM_024976866.1
Latest version!
Bos taurus RAS protein activator like 2 (RASAL2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OBa224038 XM_005217241.4
Latest version!
Bos taurus RAS protein activator like 2 (RASAL2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa224039 XM_002694157.6
Latest version!
Bos taurus RAS protein activator like 2 (RASAL2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa224040 XM_003587091.5
Latest version!
Bos taurus RAS protein activator like 2 (RASAL2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa224041 XM_024976868.1
Latest version!
Bos taurus RAS protein activator like 2 (RASAL2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa224042 XM_024976869.1
Latest version!
Bos taurus RAS protein activator like 2 (RASAL2), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa93019 XM_015466925.1
Latest version!
Bos taurus RAS protein activator like 2 (RASAL2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa93019 XM_015475217.1
Latest version!
Bos taurus RAS protein activator like 2 (RASAL2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa93020 XM_015475218.2
Latest version!
Bos taurus RAS protein activator like 2 (RASAL2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa93020 XM_015466926.1
Latest version!
Bos taurus RAS protein activator like 2 (RASAL2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa08813 XM_005217242.4
Latest version!
Bos taurus RAS protein activator like 2 (RASAL2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa08813 XM_612667.8
Latest version!
Bos taurus RAS protein activator like 2 (RASAL2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa27407 XM_015466922.1
Latest version!
Bos taurus RAS protein activator like 2 (RASAL2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa27407 XM_005217241.3
Latest version!
Bos taurus RAS protein activator like 2 (RASAL2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa27408 XM_015466923.1
Latest version!
Bos taurus RAS protein activator like 2 (RASAL2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa27408 XM_002694157.5
Latest version!
Bos taurus RAS protein activator like 2 (RASAL2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa27409 XM_003587091.4
Latest version!
Bos taurus RAS protein activator like 2 (RASAL2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa27409 XM_015466924.1
Latest version!
Bos taurus RAS protein activator like 2 (RASAL2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa27404 XM_005194886.3
Latest version!
Bos taurus RAS protein activator like 2 (RASAL2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa27404 XM_005217238.3
Latest version!
Bos taurus RAS protein activator like 2 (RASAL2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa27405 XM_005194887.3
Latest version!
Bos taurus RAS protein activator like 2 (RASAL2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa27405 XM_005217239.3
Latest version!
Bos taurus RAS protein activator like 2 (RASAL2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa27406 XM_005194888.3
Latest version!
Bos taurus RAS protein activator like 2 (RASAL2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa27406 XM_005217240.3
Latest version!
Bos taurus RAS protein activator like 2 (RASAL2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
OBa93020 XM_015475218.1 Bos taurus RAS protein activator like 2 (RASAL2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa08813 XM_005217242.3 Bos taurus RAS protein activator like 2 (RASAL2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa08809
    Clone ID Related Accession (Same CDS sequence) XM_005217238.4
    Accession Version XM_005217238.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4272bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ras GTPase-activating protein nGAP isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005217238.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAGTCCT GGAAGATGAG CATCTTTAAG AGACAGACAT CACCGTGTCC TCCAAGCTAT 
    CGAAGATCTT TGTCAGAACC TGCTTATACA CACTCAGAGA TAGGAACAAG CTTAAAGAGT
    TATCCATGGC GTGGTCCAGT GAAAAGCAAG AGGAAGAAAA ATGAACAGGC TGTTTCCAGT
    CTAGGAAAGC CACTGGGTGC TTATCGGTGG CAAACACACC ACAGCTTAAG GTTGAAAGGG
    GACAAAAAGG AGCAATTGGA AAATAATAAA GGAGACTTGG ATGAACCACC ACAACAAGCA
    GTCCTGCCAA AGATGGTTGG GGATAATATT TCTTCCTCTG GCAATGAAGT CCTTGGGACA
    TCAATGGAGA AGCAGAACCT GGTAAACACT CAGTCACTTC GGGTTCCATT CAGCAGATCT
    CTTTCAGAGC CAGAACCCGG CTCCAGTGTC AGATTTGTTA GACGGTGGCC AACTTCAGTG
    GGGTCAGTGG AGTCTGATCA GCAGGGATAT TTCATCCGTG GCATTTTCTC TACTCTTTCC
    AATGGCTTCT CCAGGATGCT GAGTCTAAAA GGAGGGTCAA CCAGTGAGCC AGTAAGAGAA
    GATGTGAAAG GGCCACCTAC CCACCGTCTG TCTTGTGGCC AGTCGCCCTA CACCGACACA
    ACAACATGGG AGCGGAAGTA CTGCATCCTC ACAGACAGCC AGCTGGTGTT GCTCAACAAG
    GAGAAGGAGA TACCTGTGGA CGGAGGACAA GAACAGCAGG CGGATTCCAC CAAAGGGCGA
    TGCCTGCGGA GAACTGTCAG TGTTCCTTCC GAGGGTCAGT TTCCTGAATA CCCACCAGAG
    GGCGCCACTA AACTGGAGGT GCCAGCAGAA AGGTCCCCAC GCAGACGGAG TATCTCAGGG
    ACCACTACAT CTGAGAAACC CAACTCTATG GACACTGCGA ATACCTCACC CTTCAAAGTA
    CCAGGGTTCT TCAGCAAGCG CCTAAAAGGC TCCATCAAGA GGACCAAAAG CCAGTCAAAG
    CTTGACCGAA ACACGAGCTT TCGGCTTCCC TCTCTTCGCA ATACCGATGA CAGGTCTCGT
    GGGCTGCCTA AACTAAAAGA GTCCCGTTCT CATGAATCTT TGCTGAGCCC ATGCAGTGCC
    GTGGAATGTC TGGATCTCGG TAGAGGGGAG CCTGTGTCGG TAAAGCCACT CCATAGCAGC
    ATCCTTGGAC AAGACTTCTG CTTTGAGGTT ACCTACTTAA GTGGAAGTAA ATGCTTCAGT
    TGTAACTCTG CTTCAGAGAG AGATAAGTGG ATGGAAAACC TGCGCAGGAC AGTTCAACCA
    AATAAGGACA ATTGTAGACG AGCTGAAAAT GTTCTCCGTT TATGGATTAT TGAAGCCAAG
    GACCTTGCCC CTAAAAAGAA ATATTTCTGT GAACTGTGCC TTGATGATAC CCTCTTTGCT
    CGTACAACTA GCAAGACCAA AGCAGACAAT ATTTTCTGGG GTGAACATTT TGAGTTCTAC
    AGCCTTCCAC CTCTTCATAG CATCACAGTT CACATTTATA AAGATGTGGA AAAAAAGAAG
    AAAAAAGACA AGAATAATTA TGTAGGGCTG GTCAACATCC CCACTGCCAG TGTGACTGGT
    CGCCAGTTTG TAGAAAAGTG GTACCCAGTG AGCACACCTA CACCCAACAA AGGAAAGACA
    GGAGGACCTT CTATTCGGAT TAAGTCGCGT TTCCAAACCA TCACCATTCT GCCTATGGAG
    CAATACAAAG AGTTTGCAGA ATTTGTCACC AGCAACTATA CCATGTTATG TTCTGTTCTT
    GAACCAGTAA TCAGTGTGAG AAATAAAGAA GAGCTAGCCT GTGCCTTAGT GCACATTCTT
    CAAAGCACTG GAAGGGCCAA GGATTTTCTG ACGGACTTGG TGATGTCTGA GGTGGATCGT
    TGTGGAGAAC ATGATGTCTT GATCTTCAGA GAGAACACCA TTGCCACCAA ATCCATTGAG
    GAATACCTCA AGTTGGTGGG ACAACAGTAT CTTCACGATG CACTGGGGGA GTTCATCAAA
    GCGTTGTATG AGTCAGATGA AAACTGTGAA GTGGATCCCA GCAAATGTTC ATCCAGTGAA
    CTTCTGGACC ATCAGAGCAA CCTGAAAATG TGCTGTGAGC TGGCTTTCTG CAAGATCATC
    AACTCTTACT GTGTTTTTCC TCGTGAGCTG AAAGAAGTGT TTGCATCCTG GAAGCAGCAG
    TGCCTGAACC GCGGCAAGCA GGACATCAGC GAGAGGCTCA TCAGCGCCTC GCTGTTTCTC
    CGTTTTCTGT GCCCGGCCAT CATGTCTCCC AGTCTCTTCA ACCTCATGCA GGAGTATCCT
    GATGACCGCA CATCTCGGAC TCTGACTCTC ATTGCCAAGG TCATCCAGAA CCTGGCCAAC
    TTTGCCAAAT TTGGTAACAA GGAGGAATAC ATGGCATTCA TGAATGATTT TTTAGAACAT
    GAATGGGGTG GAATGAAGCG CTTTCTTTTG GAGATCTCTA ATCCAGACAC CATCTCAAAC
    ACCCCAGGCT TTGATGGTTA CATTGATCTG GGCCGAGAGC TTTCAGTTTT GCATTCCTTA
    CTATGGGAAG TAGTTTCCCA ACTTGATAAG GGTGAAAATT CCTTCCTACA GGCAACCGTG
    GCAAAATTGG GACCTCTCCC TCGTGTTCTT GCTGATATTA CCAAATCTCT GACTAATCCT
    ACACCAATAC AACAGCAACT GAGACGCTTC ACTGAGCATA ACTCCAGTCC AAATGTCAGT
    GGAAGCCTCT CCTCTGGGCT GCAGAAAATA TTTGAAGACC CTACTGACAG TGATTTGCAC
    AAACTAAAAT CCCCAAGCCA GGACAACACA GACAGTTATT TCAGAGGGAA GACATTATTG
    CTGGTCCAGC AAGCCTCCTC CCAGAGCATG ACTTACTCGG AAAAGGATGA AAAGGAAAGT
    AGCCTCCCCA ATGGCCGCAG CATCTCCCTC ATGGACCTTC AGGAGACGCA CGCAGCCCAA
    GCAGAGCATC CATCTGTGAT ACTCGACGTG CCCATGCGCT TGACCGGAAG CCAGCTCTCC
    ATAACTCAGG TGGCCAGCAT CAAGCAACTG CGGGAGACCC AGAGCACCCC CCAGAGTGCA
    CCCCAAGTGC GAAGGCCCCT GCACCCAGCC TTGAACCAGC CAGGCAGCCT CCAGCCCCTG
    TCCTTCCAGA ACCCTGTCTA TCACCTCAGT AACCCCATTC CTGCAATGCC AAAGGCCTCT
    GTGGACTCCA GTTTGGAGAA CCTAAGCACT GCCAGTTCCA GGAGCCAAAG TAACAGTGAA
    GACTTCAAAC TCAGCGGACC CAGCAACAGC AGCATGGAGG ACTTCACCAA ACGGAGCACG
    CAGAGTGAGG ACTTCTCCAG GCGCCACACC ATGCCCGACA GACACATACC TCTCGCCCTG
    CCGCGGCAGA ACAGCACGGG GCAGACCCAG CTCCGGAAGA TGGACCCGGC GGGGCTGGGC
    GCCCGAGCCA AAGCCCCGCC GTCCCTGCCG CACAGCGCCT CCCTGCGGAG CACCGGGAGC
    ATGTCGGTGG CATCAGCGGC TCTGGTGGCC GAGCCCCTGC AGAACGGGAG CCGGTCCCGA
    CAGCAGTCCT CTTCCTCCAG AGAGAGCCCG GTCCCCAAAG TGAGAGCCAT CCAGAGGCAG
    CAGACCCAGC AGGTTCAGTC ACCTGTGGAC TCCGCCACAA TGTCCCCAGT CGAGAGGACA
    GCAGCCTGGG TCCTGAACAA TGGGCAGTAT GAAGAGGACG TGGAAGAGAC TGAGCAAAAT
    CAGGACGAAG CCAAGCATGC TGAGAAGTAC GAACAGGAAA TCACCAAACT GAAGGAGCGC
    CTGCGCGTGT CCAGCCGGCG ACTCGAGGAG TATGAGCGCC GGTTGCTAGT GCAGGAGCAG
    CAGATGCAGA AGCTGCTGCT GGAGTACAAG GCCCGGCTGG AGGACAGCGA GGAGCGGCTG
    CGCAGGCAGC AGGAGGAGAA GGACAGCCAG ATGAAGAGCA TCATCAGCAG GCTAATGGCT
    GTGGAAGAGG AACTGAAGAA GGATCATGCT GAGATGCAGG CAGTTATTGA TGCAAAGCAA
    AAAATAATCG ATGCACAGGT CATAAATGGA GATGAGATTA TGCAAACAGG AAAAACGGAT
    CGTGTCGCTG GATTCGGCCA ACACCAGACT GATGAGTGCG CTGACCCAGG TGAAGGAACG
    GTACAGCATG CAGGTCCGAA ATGGCATCTC CCCCACCAAC CCCACCAAGC TTTCCATCAC
    GGAGAATGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005217295.1
    CDS285..4556
    Translation

    Target ORF information:

    RefSeq Version XM_005217238.4
    Organism Bos taurus(cattle)
    Definition Bos taurus RAS protein activator like 2 (RASAL2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005217238.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAGTCCT GGAAGATGAG CATCTTTAAG AGACAGACAT CACCGTGTCC TCCAAGCTAT 
    CGAAGATCTT TGTCAGAACC TGCTTATACA CACTCAGAGA TAGGAACAAG CTTAAAGAGT
    TATCCATGGC GTGGTCCAGT GAAAAGCAAG AGGAAGAAAA ATGAACAGGC TGTTTCCAGT
    CTAGGAAAGC CACTGGGTGC TTATCGGTGG CAAACACACC ACAGCTTAAG GTTGAAAGGG
    GACAAAAAGG AGCAATTGGA AAATAATAAA GGAGACTTGG ATGAACCACC ACAACAAGCA
    GTCCTGCCAA AGATGGTTGG GGATAATATT TCTTCCTCTG GCAATGAAGT CCTTGGGACA
    TCAATGGAGA AGCAGAACCT GGTAAACACT CAGTCACTTC GGGTTCCATT CAGCAGATCT
    CTTTCAGAGC CAGAACCCGG CTCCAGTGTC AGATTTGTTA GACGGTGGCC AACTTCAGTG
    GGGTCAGTGG AGTCTGATCA GCAGGGATAT TTCATCCGTG GCATTTTCTC TACTCTTTCC
    AATGGCTTCT CCAGGATGCT GAGTCTAAAA GGAGGGTCAA CCAGTGAGCC AGTAAGAGAA
    GATGTGAAAG GGCCACCTAC CCACCGTCTG TCTTGTGGCC AGTCGCCCTA CACCGACACA
    ACAACATGGG AGCGGAAGTA CTGCATCCTC ACAGACAGCC AGCTGGTGTT GCTCAACAAG
    GAGAAGGAGA TACCTGTGGA CGGAGGACAA GAACAGCAGG CGGATTCCAC CAAAGGGCGA
    TGCCTGCGGA GAACTGTCAG TGTTCCTTCC GAGGGTCAGT TTCCTGAATA CCCACCAGAG
    GGCGCCACTA AACTGGAGGT GCCAGCAGAA AGGTCCCCAC GCAGACGGAG TATCTCAGGG
    ACCACTACAT CTGAGAAACC CAACTCTATG GACACTGCGA ATACCTCACC CTTCAAAGTA
    CCAGGGTTCT TCAGCAAGCG CCTAAAAGGC TCCATCAAGA GGACCAAAAG CCAGTCAAAG
    CTTGACCGAA ACACGAGCTT TCGGCTTCCC TCTCTTCGCA ATACCGATGA CAGGTCTCGT
    GGGCTGCCTA AACTAAAAGA GTCCCGTTCT CATGAATCTT TGCTGAGCCC ATGCAGTGCC
    GTGGAATGTC TGGATCTCGG TAGAGGGGAG CCTGTGTCGG TAAAGCCACT CCATAGCAGC
    ATCCTTGGAC AAGACTTCTG CTTTGAGGTT ACCTACTTAA GTGGAAGTAA ATGCTTCAGT
    TGTAACTCTG CTTCAGAGAG AGATAAGTGG ATGGAAAACC TGCGCAGGAC AGTTCAACCA
    AATAAGGACA ATTGTAGACG AGCTGAAAAT GTTCTCCGTT TATGGATTAT TGAAGCCAAG
    GACCTTGCCC CTAAAAAGAA ATATTTCTGT GAACTGTGCC TTGATGATAC CCTCTTTGCT
    CGTACAACTA GCAAGACCAA AGCAGACAAT ATTTTCTGGG GTGAACATTT TGAGTTCTAC
    AGCCTTCCAC CTCTTCATAG CATCACAGTT CACATTTATA AAGATGTGGA AAAAAAGAAG
    AAAAAAGACA AGAATAATTA TGTAGGGCTG GTCAACATCC CCACTGCCAG TGTGACTGGT
    CGCCAGTTTG TAGAAAAGTG GTACCCAGTG AGCACACCTA CACCCAACAA AGGAAAGACA
    GGAGGACCTT CTATTCGGAT TAAGTCGCGT TTCCAAACCA TCACCATTCT GCCTATGGAG
    CAATACAAAG AGTTTGCAGA ATTTGTCACC AGCAACTATA CCATGTTATG TTCTGTTCTT
    GAACCAGTAA TCAGTGTGAG AAATAAAGAA GAGCTAGCCT GTGCCTTAGT GCACATTCTT
    CAAAGCACTG GAAGGGCCAA GGATTTTCTG ACGGACTTGG TGATGTCTGA GGTGGATCGT
    TGTGGAGAAC ATGATGTCTT GATCTTCAGA GAGAACACCA TTGCCACCAA ATCCATTGAG
    GAATACCTCA AGTTGGTGGG ACAACAGTAT CTTCACGATG CACTGGGGGA GTTCATCAAA
    GCGTTGTATG AGTCAGATGA AAACTGTGAA GTGGATCCCA GCAAATGTTC ATCCAGTGAA
    CTTCTGGACC ATCAGAGCAA CCTGAAAATG TGCTGTGAGC TGGCTTTCTG CAAGATCATC
    AACTCTTACT GTGTTTTTCC TCGTGAGCTG AAAGAAGTGT TTGCATCCTG GAAGCAGCAG
    TGCCTGAACC GCGGCAAGCA GGACATCAGC GAGAGGCTCA TCAGCGCCTC GCTGTTTCTC
    CGTTTTCTGT GCCCGGCCAT CATGTCTCCC AGTCTCTTCA ACCTCATGCA GGAGTATCCT
    GATGACCGCA CATCTCGGAC TCTGACTCTC ATTGCCAAGG TCATCCAGAA CCTGGCCAAC
    TTTGCCAAAT TTGGTAACAA GGAGGAATAC ATGGCATTCA TGAATGATTT TTTAGAACAT
    GAATGGGGTG GAATGAAGCG CTTTCTTTTG GAGATCTCTA ATCCAGACAC CATCTCAAAC
    ACCCCAGGCT TTGATGGTTA CATTGATCTG GGCCGAGAGC TTTCAGTTTT GCATTCCTTA
    CTATGGGAAG TAGTTTCCCA ACTTGATAAG GGTGAAAATT CCTTCCTACA GGCAACCGTG
    GCAAAATTGG GACCTCTCCC TCGTGTTCTT GCTGATATTA CCAAATCTCT GACTAATCCT
    ACACCAATAC AACAGCAACT GAGACGCTTC ACTGAGCATA ACTCCAGTCC AAATGTCAGT
    GGAAGCCTCT CCTCTGGGCT GCAGAAAATA TTTGAAGACC CTACTGACAG TGATTTGCAC
    AAACTAAAAT CCCCAAGCCA GGACAACACA GACAGTTATT TCAGAGGGAA GACATTATTG
    CTGGTCCAGC AAGCCTCCTC CCAGAGCATG ACTTACTCGG AAAAGGATGA AAAGGAAAGT
    AGCCTCCCCA ATGGCCGCAG CATCTCCCTC ATGGACCTTC AGGAGACGCA CGCAGCCCAA
    GCAGAGCATC CATCTGTGAT ACTCGACGTG CCCATGCGCT TGACCGGAAG CCAGCTCTCC
    ATAACTCAGG TGGCCAGCAT CAAGCAACTG CGGGAGACCC AGAGCACCCC CCAGAGTGCA
    CCCCAAGTGC GAAGGCCCCT GCACCCAGCC TTGAACCAGC CAGGCAGCCT CCAGCCCCTG
    TCCTTCCAGA ACCCTGTCTA TCACCTCAGT AACCCCATTC CTGCAATGCC AAAGGCCTCT
    GTGGACTCCA GTTTGGAGAA CCTAAGCACT GCCAGTTCCA GGAGCCAAAG TAACAGTGAA
    GACTTCAAAC TCAGCGGACC CAGCAACAGC AGCATGGAGG ACTTCACCAA ACGGAGCACG
    CAGAGTGAGG ACTTCTCCAG GCGCCACACC ATGCCCGACA GACACATACC TCTCGCCCTG
    CCGCGGCAGA ACAGCACGGG GCAGACCCAG CTCCGGAAGA TGGACCCGGC GGGGCTGGGC
    GCCCGAGCCA AAGCCCCGCC GTCCCTGCCG CACAGCGCCT CCCTGCGGAG CACCGGGAGC
    ATGTCGGTGG CATCAGCGGC TCTGGTGGCC GAGCCCCTGC AGAACGGGAG CCGGTCCCGA
    CAGCAGTCCT CTTCCTCCAG AGAGAGCCCG GTCCCCAAAG TGAGAGCCAT CCAGAGGCAG
    CAGACCCAGC AGGTTCAGTC ACCTGTGGAC TCCGCCACAA TGTCCCCAGT CGAGAGGACA
    GCAGCCTGGG TCCTGAACAA TGGGCAGTAT GAAGAGGACG TGGAAGAGAC TGAGCAAAAT
    CAGGACGAAG CCAAGCATGC TGAGAAGTAC GAACAGGAAA TCACCAAACT GAAGGAGCGC
    CTGCGCGTGT CCAGCCGGCG ACTCGAGGAG TATGAGCGCC GGTTGCTAGT GCAGGAGCAG
    CAGATGCAGA AGCTGCTGCT GGAGTACAAG GCCCGGCTGG AGGACAGCGA GGAGCGGCTG
    CGCAGGCAGC AGGAGGAGAA GGACAGCCAG ATGAAGAGCA TCATCAGCAG GCTAATGGCT
    GTGGAAGAGG AACTGAAGAA GGATCATGCT GAGATGCAGG CAGTTATTGA TGCAAAGCAA
    AAAATAATCG ATGCACAGGT CATAAATGGA GATGAGATTA TGCAAACAGG AAAAACGGAT
    CGTGTCGCTG GATTCGGCCA ACACCAGACT GATGAGTGCG CTGACCCAGG TGAAGGAACG
    GTACAGCATG CAGGTCCGAA ATGGCATCTC CCCCACCAAC CCCACCAAGC TTTCCATCAC
    GGAGAATGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa08810
    Clone ID Related Accession (Same CDS sequence) XM_005217239.4
    Accession Version XM_005217239.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4263bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ras GTPase-activating protein nGAP isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005217239.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAGTCCT GGAAGATGAG CATCTTTAAG AGACAGACAT CACCGTGTCC TCCAAGCTAT 
    CGAAGATCTT TGTCAGAACC TGCTTATACA CACTCAGAGA TAGGAACAAG CTTAAAGAGT
    TATCCATGGC GTGGTCCAGT GAAAAGCAAG AGGAAGAAAA ATGAACAGGC TGTTTCCAGT
    CTAGGAAAGC CACTGGGTGC TTATCGGTGG CAAACACACC ACAGCTTAAG GTTGAAAGGG
    GACAAAAAGG AGCAATTGGA AAATAATAAA GGAGACTTGG ATGAACCACC ACAACAAGCA
    GTCCTGCCAA AGATGGTTGG GGATAATATT TCTTCCTCTG GCAATGAAGT CCTTGGGACA
    TCAATGGAGA AGCAGAACCT GGTAAACACT CAGTCACTTC GGGTTCCATT CAGCAGATCT
    CTTTCAGAGC CAGAACCCGG CTCCAGTGTC AGATTTGTTA GACGGTGGCC AACTTCAGTG
    GGGTCAGTGG AGTCTGATCA GCAGGGATAT TTCATCCGTG GCATTTTCTC TACTCTTTCC
    AATGGCTTCT CCAGGATGCT GAGTCTAAAA GGAGGGTCAA CCAGTGAGCC AGTAAGAGAA
    GATGTGAAAG GGCCACCTAC CCACCGTCTG TCTTGTGGCC AGTCGCCCTA CACCGACACA
    ACAACATGGG AGCGGAAGTA CTGCATCCTC ACAGACAGCC AGCTGGTGTT GCTCAACAAG
    GAGAAGGAGA TACCTGTGGA CGGAGGACAA GAACAGCAGG CGGATTCCAC CAAAGGGCGA
    TGCCTGCGGA GAACTGTCAG TGTTCCTTCC GAGGGTCAGT TTCCTGAATA CCCACCAGAG
    GGCGCCACTA AACTGGAGGT GCCAGCAGAA AGGTCCCCAC GCAGACGGAG TATCTCAGGG
    ACCACTACAT CTGAGAAACC CAACTCTATG GACACTGCGA ATACCTCACC CTTCAAAGTA
    CCAGGGTTCT TCAGCAAGCG CCTAAAAGGC TCCATCAAGA GGACCAAAAG CCAGTCAAAG
    CTTGACCGAA ACACGAGCTT TCGGCTTCCC TCTCTTCGCA ATACCGATGA CAGGTCTCGT
    GGGCTGCCTA AACTAAAAGA GTCCCGTTCT CATGAATCTT TGCTGAGCCC ATGCAGTGCC
    GTGGAATGTC TGGATCTCGG TAGAGGGGAG CCTGTGTCGG TAAAGCCACT CCATAGCAGC
    ATCCTTGGAC AAGACTTCTG CTTTGAGGTT ACCTACTTAA GTGGAAGTAA ATGCTTCAGT
    TGTAACTCTG CTTCAGAGAG AGATAAGTGG ATGGAAAACC TGCGCAGGAC AGTTCAACCA
    AATAAGGACA ATTGTAGACG AGCTGAAAAT GTTCTCCGTT TATGGATTAT TGAAGCCAAG
    GACCTTGCCC CTAAAAAGAA ATATTTCTGT GAACTGTGCC TTGATGATAC CCTCTTTGCT
    CGTACAACTA GCAAGACCAA AGCAGACAAT ATTTTCTGGG GTGAACATTT TGAGTTCTAC
    AGCCTTCCAC CTCTTCATAG CATCACAGTT CACATTTATA AAGATGTGGA AAAAAAGAAG
    AAAAAAGACA AGAATAATTA TGTAGGGCTG GTCAACATCC CCACTGCCAG TGTGACTGGT
    CGCCAGTTTG TAGAAAAGTG GTACCCAGTG AGCACACCTA CACCCAACAA AGGAAAGACA
    GGAGGACCTT CTATTCGGAT TAAGTCGCGT TTCCAAACCA TCACCATTCT GCCTATGGAG
    CAATACAAAG AGTTTGCAGA ATTTGTCACC AGCAACTATA CCATGTTATG TTCTGTTCTT
    GAACCAGTAA TCAGTGTGAG AAATAAAGAA GAGCTAGCCT GTGCCTTAGT GCACATTCTT
    CAAAGCACTG GAAGGGCCAA GGATTTTCTG ACGGACTTGG TGATGTCTGA GGTGGATCGT
    TGTGGAGAAC ATGATGTCTT GATCTTCAGA GAGAACACCA TTGCCACCAA ATCCATTGAG
    GAATACCTCA AGTTGGTGGG ACAACAGTAT CTTCACGATG CACTGGGGGA GTTCATCAAA
    GCGTTGTATG AGTCAGATGA AAACTGTGAA GTGGATCCCA GCAAATGTTC ATCCAGTGAA
    CTTCTGGACC ATCAGAGCAA CCTGAAAATG TGCTGTGAGC TGGCTTTCTG CAAGATCATC
    AACTCTTACT GTGTTTTTCC TCGTGAGCTG AAAGAAGTGT TTGCATCCTG GAAGCAGCAG
    TGCCTGAACC GCGGCAAGCA GGACATCAGC GAGAGGCTCA TCAGCGCCTC GCTGTTTCTC
    CGTTTTCTGT GCCCGGCCAT CATGTCTCCC AGTCTCTTCA ACCTCATGCA GGAGTATCCT
    GATGACCGCA CATCTCGGAC TCTGACTCTC ATTGCCAAGG TCATCCAGAA CCTGGCCAAC
    TTTGCCAAAT TTGGTAACAA GGAGGAATAC ATGGCATTCA TGAATGATTT TTTAGAACAT
    GAATGGGGTG GAATGAAGCG CTTTCTTTTG GAGATCTCTA ATCCAGACAC CATCTCAAAC
    ACCCCAGGCT TTGATGGTTA CATTGATCTG GGCCGAGAGC TTTCAGTTTT GCATTCCTTA
    CTATGGGAAG TAGTTTCCCA ACTTGATAAG GGTGAAAATT CCTTCCTACA GGCAACCGTG
    GCAAAATTGG GACCTCTCCC TCGTGTTCTT GCTGATATTA CCAAATCTCT GACTAATCCT
    ACACCAATAC AACAGCAACT GAGACGCTTC ACTGAGCATA ACTCCAGTCC AAATGTCAGT
    GGAAGCCTCT CCTCTGGGCT GCAGAAAATA TTTGAAGACC CTACTGACAG TGATTTGCAC
    AAACTAAAAT CCCCAAGCCA GGACAACACA GACAGTTATT TCAGAGGGAA GACATTATTG
    CTGGTCCAGC AAGCCTCCTC CCAGAGCATG ACTTACTCGG AAAAGGATGA AAAGGAAAGT
    AGCCTCCCCA ATGGCCGCAG CATCTCCCTC ATGGACCTTC AGGAGACGCA CGCAGCCCAA
    GCAGAGCATC CATCTGTGAT ACTCGACGTG CCCATGCGCT TGACCGGAAG CCAGCTCTCC
    ATAACTCAGG TGGCCAGCAT CAAGCAACTG CGGGAGACCC AGAGCACCCC CCAGAGTGCA
    CCCCAAGTGC GAAGGCCCCT GCACCCAGCC TTGAACCAGC CAGGCAGCCT CCAGCCCCTG
    TCCTTCCAGA ACCCTGTCTA TCACCTCAGT AACCCCATTC CTGCAATGCC AAAGGCCTCT
    GTGGACTCCA GTTTGGAGAA CCTAAGCACT GCCAGTTCCA GGAGCCAAAG TAACAGTGAA
    GACTTCAAAC TCAGCGGACC CAGCAACAGC AGCATGGAGG ACTTCACCAA ACGGAGCACG
    CAGAGTGAGG ACTTCTCCAG GCGCCACACC ATGCCCGACA GACACATACC TCTCGCCCTG
    CCGCGGCAGA ACAGCACGGG GCAGACCCAG CTCCGGAAGA TGGACCCGGC GGGGCTGGGC
    GCCCGAGCCA AAGCCCCGCC GTCCCTGCCG CACAGCGCCT CCCTGCGGAG CACCGGGAGC
    ATGTCGGTGG CATCAGCGGC TCTGGTGGCC GAGCCCCTGC AGAACGGGAG CCGGTCCCGA
    CAGCAGTCCT CTTCCTCCAG AGAGAGCCCG GTCCCCAAAG TGAGAGCCAT CCAGAGGCAG
    CAGACCCAGC AGGTTCAGTC ACCTGTGGAC TCCGCCACAA TGTCCCCAGT CGAGAGGACA
    GCAGCCTGGG TCCTGAACAA TGGGCAGTAT GAAGAGGACG TGGAAGAGAC TGAGCAAAAT
    CAGGACGAAG CCAAGCATGC TGAGAAGTAC GAACAGGAAA TCACCAAACT GAAGGAGCGC
    CTGCGCGTGT CCAGCCGGCG ACTCGAGGAG TATGAGCGCC GGTTGCTAGT GCAGGAGCAG
    CAGATGCAGA AGCTGCTGCT GGAGTACAAG GCCCGGCTGG AGGACAGCGA GGAGCGGCTG
    CGCAGGCAGC AGGAGGAGAA GGACAGCCAG ATGAAGAGCA TCATCAGCAG GCTAATGGCT
    GTGGAAGAGG AACTGAAGAA GGATCATGCT GAGATGCAGG CAGTTATTGA TGCAAAGCAA
    AAAATAATCG ATGCACAGGA AAAACGGATC GTGTCGCTGG ATTCGGCCAA CACCAGACTG
    ATGAGTGCGC TGACCCAGGT GAAGGAACGG TACAGCATGC AGGTCCGAAA TGGCATCTCC
    CCCACCAACC CCACCAAGCT TTCCATCACG GAGAATGGTG AATTCAAAAA CAGCAGCTGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005217296.1
    CDS284..4546
    Translation

    Target ORF information:

    RefSeq Version XM_005217239.4
    Organism Bos taurus(cattle)
    Definition Bos taurus RAS protein activator like 2 (RASAL2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005217239.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAGTCCT GGAAGATGAG CATCTTTAAG AGACAGACAT CACCGTGTCC TCCAAGCTAT 
    CGAAGATCTT TGTCAGAACC TGCTTATACA CACTCAGAGA TAGGAACAAG CTTAAAGAGT
    TATCCATGGC GTGGTCCAGT GAAAAGCAAG AGGAAGAAAA ATGAACAGGC TGTTTCCAGT
    CTAGGAAAGC CACTGGGTGC TTATCGGTGG CAAACACACC ACAGCTTAAG GTTGAAAGGG
    GACAAAAAGG AGCAATTGGA AAATAATAAA GGAGACTTGG ATGAACCACC ACAACAAGCA
    GTCCTGCCAA AGATGGTTGG GGATAATATT TCTTCCTCTG GCAATGAAGT CCTTGGGACA
    TCAATGGAGA AGCAGAACCT GGTAAACACT CAGTCACTTC GGGTTCCATT CAGCAGATCT
    CTTTCAGAGC CAGAACCCGG CTCCAGTGTC AGATTTGTTA GACGGTGGCC AACTTCAGTG
    GGGTCAGTGG AGTCTGATCA GCAGGGATAT TTCATCCGTG GCATTTTCTC TACTCTTTCC
    AATGGCTTCT CCAGGATGCT GAGTCTAAAA GGAGGGTCAA CCAGTGAGCC AGTAAGAGAA
    GATGTGAAAG GGCCACCTAC CCACCGTCTG TCTTGTGGCC AGTCGCCCTA CACCGACACA
    ACAACATGGG AGCGGAAGTA CTGCATCCTC ACAGACAGCC AGCTGGTGTT GCTCAACAAG
    GAGAAGGAGA TACCTGTGGA CGGAGGACAA GAACAGCAGG CGGATTCCAC CAAAGGGCGA
    TGCCTGCGGA GAACTGTCAG TGTTCCTTCC GAGGGTCAGT TTCCTGAATA CCCACCAGAG
    GGCGCCACTA AACTGGAGGT GCCAGCAGAA AGGTCCCCAC GCAGACGGAG TATCTCAGGG
    ACCACTACAT CTGAGAAACC CAACTCTATG GACACTGCGA ATACCTCACC CTTCAAAGTA
    CCAGGGTTCT TCAGCAAGCG CCTAAAAGGC TCCATCAAGA GGACCAAAAG CCAGTCAAAG
    CTTGACCGAA ACACGAGCTT TCGGCTTCCC TCTCTTCGCA ATACCGATGA CAGGTCTCGT
    GGGCTGCCTA AACTAAAAGA GTCCCGTTCT CATGAATCTT TGCTGAGCCC ATGCAGTGCC
    GTGGAATGTC TGGATCTCGG TAGAGGGGAG CCTGTGTCGG TAAAGCCACT CCATAGCAGC
    ATCCTTGGAC AAGACTTCTG CTTTGAGGTT ACCTACTTAA GTGGAAGTAA ATGCTTCAGT
    TGTAACTCTG CTTCAGAGAG AGATAAGTGG ATGGAAAACC TGCGCAGGAC AGTTCAACCA
    AATAAGGACA ATTGTAGACG AGCTGAAAAT GTTCTCCGTT TATGGATTAT TGAAGCCAAG
    GACCTTGCCC CTAAAAAGAA ATATTTCTGT GAACTGTGCC TTGATGATAC CCTCTTTGCT
    CGTACAACTA GCAAGACCAA AGCAGACAAT ATTTTCTGGG GTGAACATTT TGAGTTCTAC
    AGCCTTCCAC CTCTTCATAG CATCACAGTT CACATTTATA AAGATGTGGA AAAAAAGAAG
    AAAAAAGACA AGAATAATTA TGTAGGGCTG GTCAACATCC CCACTGCCAG TGTGACTGGT
    CGCCAGTTTG TAGAAAAGTG GTACCCAGTG AGCACACCTA CACCCAACAA AGGAAAGACA
    GGAGGACCTT CTATTCGGAT TAAGTCGCGT TTCCAAACCA TCACCATTCT GCCTATGGAG
    CAATACAAAG AGTTTGCAGA ATTTGTCACC AGCAACTATA CCATGTTATG TTCTGTTCTT
    GAACCAGTAA TCAGTGTGAG AAATAAAGAA GAGCTAGCCT GTGCCTTAGT GCACATTCTT
    CAAAGCACTG GAAGGGCCAA GGATTTTCTG ACGGACTTGG TGATGTCTGA GGTGGATCGT
    TGTGGAGAAC ATGATGTCTT GATCTTCAGA GAGAACACCA TTGCCACCAA ATCCATTGAG
    GAATACCTCA AGTTGGTGGG ACAACAGTAT CTTCACGATG CACTGGGGGA GTTCATCAAA
    GCGTTGTATG AGTCAGATGA AAACTGTGAA GTGGATCCCA GCAAATGTTC ATCCAGTGAA
    CTTCTGGACC ATCAGAGCAA CCTGAAAATG TGCTGTGAGC TGGCTTTCTG CAAGATCATC
    AACTCTTACT GTGTTTTTCC TCGTGAGCTG AAAGAAGTGT TTGCATCCTG GAAGCAGCAG
    TGCCTGAACC GCGGCAAGCA GGACATCAGC GAGAGGCTCA TCAGCGCCTC GCTGTTTCTC
    CGTTTTCTGT GCCCGGCCAT CATGTCTCCC AGTCTCTTCA ACCTCATGCA GGAGTATCCT
    GATGACCGCA CATCTCGGAC TCTGACTCTC ATTGCCAAGG TCATCCAGAA CCTGGCCAAC
    TTTGCCAAAT TTGGTAACAA GGAGGAATAC ATGGCATTCA TGAATGATTT TTTAGAACAT
    GAATGGGGTG GAATGAAGCG CTTTCTTTTG GAGATCTCTA ATCCAGACAC CATCTCAAAC
    ACCCCAGGCT TTGATGGTTA CATTGATCTG GGCCGAGAGC TTTCAGTTTT GCATTCCTTA
    CTATGGGAAG TAGTTTCCCA ACTTGATAAG GGTGAAAATT CCTTCCTACA GGCAACCGTG
    GCAAAATTGG GACCTCTCCC TCGTGTTCTT GCTGATATTA CCAAATCTCT GACTAATCCT
    ACACCAATAC AACAGCAACT GAGACGCTTC ACTGAGCATA ACTCCAGTCC AAATGTCAGT
    GGAAGCCTCT CCTCTGGGCT GCAGAAAATA TTTGAAGACC CTACTGACAG TGATTTGCAC
    AAACTAAAAT CCCCAAGCCA GGACAACACA GACAGTTATT TCAGAGGGAA GACATTATTG
    CTGGTCCAGC AAGCCTCCTC CCAGAGCATG ACTTACTCGG AAAAGGATGA AAAGGAAAGT
    AGCCTCCCCA ATGGCCGCAG CATCTCCCTC ATGGACCTTC AGGAGACGCA CGCAGCCCAA
    GCAGAGCATC CATCTGTGAT ACTCGACGTG CCCATGCGCT TGACCGGAAG CCAGCTCTCC
    ATAACTCAGG TGGCCAGCAT CAAGCAACTG CGGGAGACCC AGAGCACCCC CCAGAGTGCA
    CCCCAAGTGC GAAGGCCCCT GCACCCAGCC TTGAACCAGC CAGGCAGCCT CCAGCCCCTG
    TCCTTCCAGA ACCCTGTCTA TCACCTCAGT AACCCCATTC CTGCAATGCC AAAGGCCTCT
    GTGGACTCCA GTTTGGAGAA CCTAAGCACT GCCAGTTCCA GGAGCCAAAG TAACAGTGAA
    GACTTCAAAC TCAGCGGACC CAGCAACAGC AGCATGGAGG ACTTCACCAA ACGGAGCACG
    CAGAGTGAGG ACTTCTCCAG GCGCCACACC ATGCCCGACA GACACATACC TCTCGCCCTG
    CCGCGGCAGA ACAGCACGGG GCAGACCCAG CTCCGGAAGA TGGACCCGGC GGGGCTGGGC
    GCCCGAGCCA AAGCCCCGCC GTCCCTGCCG CACAGCGCCT CCCTGCGGAG CACCGGGAGC
    ATGTCGGTGG CATCAGCGGC TCTGGTGGCC GAGCCCCTGC AGAACGGGAG CCGGTCCCGA
    CAGCAGTCCT CTTCCTCCAG AGAGAGCCCG GTCCCCAAAG TGAGAGCCAT CCAGAGGCAG
    CAGACCCAGC AGGTTCAGTC ACCTGTGGAC TCCGCCACAA TGTCCCCAGT CGAGAGGACA
    GCAGCCTGGG TCCTGAACAA TGGGCAGTAT GAAGAGGACG TGGAAGAGAC TGAGCAAAAT
    CAGGACGAAG CCAAGCATGC TGAGAAGTAC GAACAGGAAA TCACCAAACT GAAGGAGCGC
    CTGCGCGTGT CCAGCCGGCG ACTCGAGGAG TATGAGCGCC GGTTGCTAGT GCAGGAGCAG
    CAGATGCAGA AGCTGCTGCT GGAGTACAAG GCCCGGCTGG AGGACAGCGA GGAGCGGCTG
    CGCAGGCAGC AGGAGGAGAA GGACAGCCAG ATGAAGAGCA TCATCAGCAG GCTAATGGCT
    GTGGAAGAGG AACTGAAGAA GGATCATGCT GAGATGCAGG CAGTTATTGA TGCAAAGCAA
    AAAATAATCG ATGCACAGGA AAAACGGATC GTGTCGCTGG ATTCGGCCAA CACCAGACTG
    ATGAGTGCGC TGACCCAGGT GAAGGAACGG TACAGCATGC AGGTCCGAAA TGGCATCTCC
    CCCACCAACC CCACCAAGCT TTCCATCACG GAGAATGGTG AATTCAAAAA CAGCAGCTGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa08811
    Clone ID Related Accession (Same CDS sequence) XM_005217240.4
    Accession Version XM_005217240.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4251bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ras GTPase-activating protein nGAP isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005217240.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGAGTCCT GGAAGATGAG CATCTTTAAG AGACAGACAT CACCGTGTCC TCCAAGCTAT 
    CGAAGATCTT TGTCAGAACC TGCTTATACA CACTCAGAGA TAGGAACAAG CTTAAAGAGT
    TATCCATGGC GTGGTCCAGT GAAAAGCAAG AGGAAGAAAA ATGAACAGGC TGTTTCCAGT
    CTAGGAAAGC CACTGGGTGC TTATCGGTGG CAAACACACC ACAGCTTAAG GTTGAAAGGG
    GACAAAAAGG AGCAATTGGA AAATAATAAA GGAGACTTGG ATGAACCACC ACAACAAGCA
    GTCCTGCCAA AGATGGTTGG GGATAATATT TCTTCCTCTG GCAATGAAGT CCTTGGGACA
    TCAATGGAGA AGCAGAACCT GGTAAACACT CAGTCACTTC GGGTTCCATT CAGCAGATCT
    CTTTCAGAGC CAGAACCCGG CTCCAGTGTC AGATTTGTTA GACGGTGGCC AACTTCAGTG
    GGGTCAGTGG AGTCTGATCA GCAGGGATAT TTCATCCGTG GCATTTTCTC TACTCTTTCC
    AATGGCTTCT CCAGGATGCT GAGTCTAAAA GGAGGGTCAA CCAGTGAGCC AGTAAGAGAA
    GATGTGAAAG GGCCACCTAC CCACCGTCTG TCTTGTGGCC AGTCGCCCTA CACCGACACA
    ACAACATGGG AGCGGAAGTA CTGCATCCTC ACAGACAGCC AGCTGGTGTT GCTCAACAAG
    GAGAAGGAGA TACCTGTGGA CGGAGGACAA GAACAGCAGG CGGATTCCAC CAAAGGGCGA
    TGCCTGCGGA GAACTGTCAG TGTTCCTTCC GAGGGTCAGT TTCCTGAATA CCCACCAGAG
    GGCGCCACTA AACTGGAGGT GCCAGCAGAA AGGTCCCCAC GCAGACGGAG TATCTCAGGG
    ACCACTACAT CTGAGAAACC CAACTCTATG GACACTGCGA ATACCTCACC CTTCAAAGTA
    CCAGGGTTCT TCAGCAAGCG CCTAAAAGGC TCCATCAAGA GGACCAAAAG CCAGTCAAAG
    CTTGACCGAA ACACGAGCTT TCGGCTTCCC TCTCTTCGCA ATACCGATGA CAGGTCTCGT
    GGGCTGCCTA AACTAAAAGA GTCCCGTTCT CATGAATCTT TGCTGAGCCC ATGCAGTGCC
    GTGGAATGTC TGGATCTCGG TAGAGGGGAG CCTGTGTCGG TAAAGCCACT CCATAGCAGC
    ATCCTTGGAC AAGACTTCTG CTTTGAGGTT ACCTACTTAA GTGGAAGTAA ATGCTTCAGT
    TGTAACTCTG CTTCAGAGAG AGATAAGTGG ATGGAAAACC TGCGCAGGAC AGTTCAACCA
    AATAAGGACA ATTGTAGACG AGCTGAAAAT GTTCTCCGTT TATGGATTAT TGAAGCCAAG
    GACCTTGCCC CTAAAAAGAA ATATTTCTGT GAACTGTGCC TTGATGATAC CCTCTTTGCT
    CGTACAACTA GCAAGACCAA AGCAGACAAT ATTTTCTGGG GTGAACATTT TGAGTTCTAC
    AGCCTTCCAC CTCTTCATAG CATCACAGTT CACATTTATA AAGATGTGGA AAAAAAGAAG
    AAAAAAGACA AGAATAATTA TGTAGGGCTG GTCAACATCC CCACTGCCAG TGTGACTGGT
    CGCCAGTTTG TAGAAAAGTG GTACCCAGTG AGCACACCTA CACCCAACAA AGGAAAGACA
    GGAGGACCTT CTATTCGGAT TAAGTCGCGT TTCCAAACCA TCACCATTCT GCCTATGGAG
    CAATACAAAG AGTTTGCAGA ATTTGTCACC AGCAACTATA CCATGTTATG TTCTGTTCTT
    GAACCAGTAA TCAGTGTGAG AAATAAAGAA GAGCTAGCCT GTGCCTTAGT GCACATTCTT
    CAAAGCACTG GAAGGGCCAA GGATTTTCTG ACGGACTTGG TGATGTCTGA GGTGGATCGT
    TGTGGAGAAC ATGATGTCTT GATCTTCAGA GAGAACACCA TTGCCACCAA ATCCATTGAG
    GAATACCTCA AGTTGGTGGG ACAACAGTAT CTTCACGATG CACTGGGGGA GTTCATCAAA
    GCGTTGTATG AGTCAGATGA AAACTGTGAA GTGGATCCCA GCAAATGTTC ATCCAGTGAA
    CTTCTGGACC ATCAGAGCAA CCTGAAAATG TGCTGTGAGC TGGCTTTCTG CAAGATCATC
    AACTCTTACT GTGTTTTTCC TCGTGAGCTG AAAGAAGTGT TTGCATCCTG GAAGCAGCAG
    TGCCTGAACC GCGGCAAGCA GGACATCAGC GAGAGGCTCA TCAGCGCCTC GCTGTTTCTC
    CGTTTTCTGT GCCCGGCCAT CATGTCTCCC AGTCTCTTCA ACCTCATGCA GGAGTATCCT
    GATGACCGCA CATCTCGGAC TCTGACTCTC ATTGCCAAGG TCATCCAGAA CCTGGCCAAC
    TTTGCCAAAT TTGGTAACAA GGAGGAATAC ATGGCATTCA TGAATGATTT TTTAGAACAT
    GAATGGGGTG GAATGAAGCG CTTTCTTTTG GAGATCTCTA ATCCAGACAC CATCTCAAAC
    ACCCCAGGCT TTGATGGTTA CATTGATCTG GGCCGAGAGC TTTCAGTTTT GCATTCCTTA
    CTATGGGAAG TAGTTTCCCA ACTTGATAAG GCAACCGTGG CAAAATTGGG ACCTCTCCCT
    CGTGTTCTTG CTGATATTAC CAAATCTCTG ACTAATCCTA CACCAATACA ACAGCAACTG
    AGACGCTTCA CTGAGCATAA CTCCAGTCCA AATGTCAGTG GAAGCCTCTC CTCTGGGCTG
    CAGAAAATAT TTGAAGACCC TACTGACAGT GATTTGCACA AACTAAAATC CCCAAGCCAG
    GACAACACAG ACAGTTATTT CAGAGGGAAG ACATTATTGC TGGTCCAGCA AGCCTCCTCC
    CAGAGCATGA CTTACTCGGA AAAGGATGAA AAGGAAAGTA GCCTCCCCAA TGGCCGCAGC
    ATCTCCCTCA TGGACCTTCA GGAGACGCAC GCAGCCCAAG CAGAGCATCC ATCTGTGATA
    CTCGACGTGC CCATGCGCTT GACCGGAAGC CAGCTCTCCA TAACTCAGGT GGCCAGCATC
    AAGCAACTGC GGGAGACCCA GAGCACCCCC CAGAGTGCAC CCCAAGTGCG AAGGCCCCTG
    CACCCAGCCT TGAACCAGCC AGGCAGCCTC CAGCCCCTGT CCTTCCAGAA CCCTGTCTAT
    CACCTCAGTA ACCCCATTCC TGCAATGCCA AAGGCCTCTG TGGACTCCAG TTTGGAGAAC
    CTAAGCACTG CCAGTTCCAG GAGCCAAAGT AACAGTGAAG ACTTCAAACT CAGCGGACCC
    AGCAACAGCA GCATGGAGGA CTTCACCAAA CGGAGCACGC AGAGTGAGGA CTTCTCCAGG
    CGCCACACCA TGCCCGACAG ACACATACCT CTCGCCCTGC CGCGGCAGAA CAGCACGGGG
    CAGACCCAGC TCCGGAAGAT GGACCCGGCG GGGCTGGGCG CCCGAGCCAA AGCCCCGCCG
    TCCCTGCCGC ACAGCGCCTC CCTGCGGAGC ACCGGGAGCA TGTCGGTGGC ATCAGCGGCT
    CTGGTGGCCG AGCCCCTGCA GAACGGGAGC CGGTCCCGAC AGCAGTCCTC TTCCTCCAGA
    GAGAGCCCGG TCCCCAAAGT GAGAGCCATC CAGAGGCAGC AGACCCAGCA GGTTCAGTCA
    CCTGTGGACT CCGCCACAAT GTCCCCAGTC GAGAGGACAG CAGCCTGGGT CCTGAACAAT
    GGGCAGTATG AAGAGGACGT GGAAGAGACT GAGCAAAATC AGGACGAAGC CAAGCATGCT
    GAGAAGTACG AACAGGAAAT CACCAAACTG AAGGAGCGCC TGCGCGTGTC CAGCCGGCGA
    CTCGAGGAGT ATGAGCGCCG GTTGCTAGTG CAGGAGCAGC AGATGCAGAA GCTGCTGCTG
    GAGTACAAGG CCCGGCTGGA GGACAGCGAG GAGCGGCTGC GCAGGCAGCA GGAGGAGAAG
    GACAGCCAGA TGAAGAGCAT CATCAGCAGG CTAATGGCTG TGGAAGAGGA ACTGAAGAAG
    GATCATGCTG AGATGCAGGC AGTTATTGAT GCAAAGCAAA AAATAATCGA TGCACAGGTC
    ATAAATGGAG ATGAGATTAT GCAAACAGGA AAAACGGATC GTGTCGCTGG ATTCGGCCAA
    CACCAGACTG ATGAGTGCGC TGACCCAGGT GAAGGAACGG TACAGCATGC AGGTCCGAAA
    TGGCATCTCC CCCACCAACC CCACCAAGCT TTCCATCACG GAGAATGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005217297.1
    CDS287..4537
    Translation

    Target ORF information:

    RefSeq Version XM_005217240.4
    Organism Bos taurus(cattle)
    Definition Bos taurus RAS protein activator like 2 (RASAL2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005217240.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGAGTCCT GGAAGATGAG CATCTTTAAG AGACAGACAT CACCGTGTCC TCCAAGCTAT 
    CGAAGATCTT TGTCAGAACC TGCTTATACA CACTCAGAGA TAGGAACAAG CTTAAAGAGT
    TATCCATGGC GTGGTCCAGT GAAAAGCAAG AGGAAGAAAA ATGAACAGGC TGTTTCCAGT
    CTAGGAAAGC CACTGGGTGC TTATCGGTGG CAAACACACC ACAGCTTAAG GTTGAAAGGG
    GACAAAAAGG AGCAATTGGA AAATAATAAA GGAGACTTGG ATGAACCACC ACAACAAGCA
    GTCCTGCCAA AGATGGTTGG GGATAATATT TCTTCCTCTG GCAATGAAGT CCTTGGGACA
    TCAATGGAGA AGCAGAACCT GGTAAACACT CAGTCACTTC GGGTTCCATT CAGCAGATCT
    CTTTCAGAGC CAGAACCCGG CTCCAGTGTC AGATTTGTTA GACGGTGGCC AACTTCAGTG
    GGGTCAGTGG AGTCTGATCA GCAGGGATAT TTCATCCGTG GCATTTTCTC TACTCTTTCC
    AATGGCTTCT CCAGGATGCT GAGTCTAAAA GGAGGGTCAA CCAGTGAGCC AGTAAGAGAA
    GATGTGAAAG GGCCACCTAC CCACCGTCTG TCTTGTGGCC AGTCGCCCTA CACCGACACA
    ACAACATGGG AGCGGAAGTA CTGCATCCTC ACAGACAGCC AGCTGGTGTT GCTCAACAAG
    GAGAAGGAGA TACCTGTGGA CGGAGGACAA GAACAGCAGG CGGATTCCAC CAAAGGGCGA
    TGCCTGCGGA GAACTGTCAG TGTTCCTTCC GAGGGTCAGT TTCCTGAATA CCCACCAGAG
    GGCGCCACTA AACTGGAGGT GCCAGCAGAA AGGTCCCCAC GCAGACGGAG TATCTCAGGG
    ACCACTACAT CTGAGAAACC CAACTCTATG GACACTGCGA ATACCTCACC CTTCAAAGTA
    CCAGGGTTCT TCAGCAAGCG CCTAAAAGGC TCCATCAAGA GGACCAAAAG CCAGTCAAAG
    CTTGACCGAA ACACGAGCTT TCGGCTTCCC TCTCTTCGCA ATACCGATGA CAGGTCTCGT
    GGGCTGCCTA AACTAAAAGA GTCCCGTTCT CATGAATCTT TGCTGAGCCC ATGCAGTGCC
    GTGGAATGTC TGGATCTCGG TAGAGGGGAG CCTGTGTCGG TAAAGCCACT CCATAGCAGC
    ATCCTTGGAC AAGACTTCTG CTTTGAGGTT ACCTACTTAA GTGGAAGTAA ATGCTTCAGT
    TGTAACTCTG CTTCAGAGAG AGATAAGTGG ATGGAAAACC TGCGCAGGAC AGTTCAACCA
    AATAAGGACA ATTGTAGACG AGCTGAAAAT GTTCTCCGTT TATGGATTAT TGAAGCCAAG
    GACCTTGCCC CTAAAAAGAA ATATTTCTGT GAACTGTGCC TTGATGATAC CCTCTTTGCT
    CGTACAACTA GCAAGACCAA AGCAGACAAT ATTTTCTGGG GTGAACATTT TGAGTTCTAC
    AGCCTTCCAC CTCTTCATAG CATCACAGTT CACATTTATA AAGATGTGGA AAAAAAGAAG
    AAAAAAGACA AGAATAATTA TGTAGGGCTG GTCAACATCC CCACTGCCAG TGTGACTGGT
    CGCCAGTTTG TAGAAAAGTG GTACCCAGTG AGCACACCTA CACCCAACAA AGGAAAGACA
    GGAGGACCTT CTATTCGGAT TAAGTCGCGT TTCCAAACCA TCACCATTCT GCCTATGGAG
    CAATACAAAG AGTTTGCAGA ATTTGTCACC AGCAACTATA CCATGTTATG TTCTGTTCTT
    GAACCAGTAA TCAGTGTGAG AAATAAAGAA GAGCTAGCCT GTGCCTTAGT GCACATTCTT
    CAAAGCACTG GAAGGGCCAA GGATTTTCTG ACGGACTTGG TGATGTCTGA GGTGGATCGT
    TGTGGAGAAC ATGATGTCTT GATCTTCAGA GAGAACACCA TTGCCACCAA ATCCATTGAG
    GAATACCTCA AGTTGGTGGG ACAACAGTAT CTTCACGATG CACTGGGGGA GTTCATCAAA
    GCGTTGTATG AGTCAGATGA AAACTGTGAA GTGGATCCCA GCAAATGTTC ATCCAGTGAA
    CTTCTGGACC ATCAGAGCAA CCTGAAAATG TGCTGTGAGC TGGCTTTCTG CAAGATCATC
    AACTCTTACT GTGTTTTTCC TCGTGAGCTG AAAGAAGTGT TTGCATCCTG GAAGCAGCAG
    TGCCTGAACC GCGGCAAGCA GGACATCAGC GAGAGGCTCA TCAGCGCCTC GCTGTTTCTC
    CGTTTTCTGT GCCCGGCCAT CATGTCTCCC AGTCTCTTCA ACCTCATGCA GGAGTATCCT
    GATGACCGCA CATCTCGGAC TCTGACTCTC ATTGCCAAGG TCATCCAGAA CCTGGCCAAC
    TTTGCCAAAT TTGGTAACAA GGAGGAATAC ATGGCATTCA TGAATGATTT TTTAGAACAT
    GAATGGGGTG GAATGAAGCG CTTTCTTTTG GAGATCTCTA ATCCAGACAC CATCTCAAAC
    ACCCCAGGCT TTGATGGTTA CATTGATCTG GGCCGAGAGC TTTCAGTTTT GCATTCCTTA
    CTATGGGAAG TAGTTTCCCA ACTTGATAAG GCAACCGTGG CAAAATTGGG ACCTCTCCCT
    CGTGTTCTTG CTGATATTAC CAAATCTCTG ACTAATCCTA CACCAATACA ACAGCAACTG
    AGACGCTTCA CTGAGCATAA CTCCAGTCCA AATGTCAGTG GAAGCCTCTC CTCTGGGCTG
    CAGAAAATAT TTGAAGACCC TACTGACAGT GATTTGCACA AACTAAAATC CCCAAGCCAG
    GACAACACAG ACAGTTATTT CAGAGGGAAG ACATTATTGC TGGTCCAGCA AGCCTCCTCC
    CAGAGCATGA CTTACTCGGA AAAGGATGAA AAGGAAAGTA GCCTCCCCAA TGGCCGCAGC
    ATCTCCCTCA TGGACCTTCA GGAGACGCAC GCAGCCCAAG CAGAGCATCC ATCTGTGATA
    CTCGACGTGC CCATGCGCTT GACCGGAAGC CAGCTCTCCA TAACTCAGGT GGCCAGCATC
    AAGCAACTGC GGGAGACCCA GAGCACCCCC CAGAGTGCAC CCCAAGTGCG AAGGCCCCTG
    CACCCAGCCT TGAACCAGCC AGGCAGCCTC CAGCCCCTGT CCTTCCAGAA CCCTGTCTAT
    CACCTCAGTA ACCCCATTCC TGCAATGCCA AAGGCCTCTG TGGACTCCAG TTTGGAGAAC
    CTAAGCACTG CCAGTTCCAG GAGCCAAAGT AACAGTGAAG ACTTCAAACT CAGCGGACCC
    AGCAACAGCA GCATGGAGGA CTTCACCAAA CGGAGCACGC AGAGTGAGGA CTTCTCCAGG
    CGCCACACCA TGCCCGACAG ACACATACCT CTCGCCCTGC CGCGGCAGAA CAGCACGGGG
    CAGACCCAGC TCCGGAAGAT GGACCCGGCG GGGCTGGGCG CCCGAGCCAA AGCCCCGCCG
    TCCCTGCCGC ACAGCGCCTC CCTGCGGAGC ACCGGGAGCA TGTCGGTGGC ATCAGCGGCT
    CTGGTGGCCG AGCCCCTGCA GAACGGGAGC CGGTCCCGAC AGCAGTCCTC TTCCTCCAGA
    GAGAGCCCGG TCCCCAAAGT GAGAGCCATC CAGAGGCAGC AGACCCAGCA GGTTCAGTCA
    CCTGTGGACT CCGCCACAAT GTCCCCAGTC GAGAGGACAG CAGCCTGGGT CCTGAACAAT
    GGGCAGTATG AAGAGGACGT GGAAGAGACT GAGCAAAATC AGGACGAAGC CAAGCATGCT
    GAGAAGTACG AACAGGAAAT CACCAAACTG AAGGAGCGCC TGCGCGTGTC CAGCCGGCGA
    CTCGAGGAGT ATGAGCGCCG GTTGCTAGTG CAGGAGCAGC AGATGCAGAA GCTGCTGCTG
    GAGTACAAGG CCCGGCTGGA GGACAGCGAG GAGCGGCTGC GCAGGCAGCA GGAGGAGAAG
    GACAGCCAGA TGAAGAGCAT CATCAGCAGG CTAATGGCTG TGGAAGAGGA ACTGAAGAAG
    GATCATGCTG AGATGCAGGC AGTTATTGAT GCAAAGCAAA AAATAATCGA TGCACAGGTC
    ATAAATGGAG ATGAGATTAT GCAAACAGGA AAAACGGATC GTGTCGCTGG ATTCGGCCAA
    CACCAGACTG ATGAGTGCGC TGACCCAGGT GAAGGAACGG TACAGCATGC AGGTCCGAAA
    TGGCATCTCC CCCACCAACC CCACCAAGCT TTCCATCACG GAGAATGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa08812
    Clone ID Related Accession (Same CDS sequence) XM_024976866.1
    Accession Version XM_024976866.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4242bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ras GTPase-activating protein nGAP isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGAGTCCT GGAAGATGAG CATCTTTAAG AGACAGACAT CACCGTGTCC TCCAAGCTAT 
    CGAAGATCTT TGTCAGAACC TGCTTATACA CACTCAGAGA TAGGAACAAG CTTAAAGAGT
    TATCCATGGC GTGGTCCAGT GAAAAGCAAG AGGAAGAAAA ATGAACAGGC TGTTTCCAGT
    CTAGGAAAGC CACTGGGTGC TTATCGGTGG CAAACACACC ACAGCTTAAG GTTGAAAGGG
    GACAAAAAGG AGCAATTGGA AAATAATAAA GGAGACTTGG ATGAACCACC ACAACAAGCA
    GTCCTGCCAA AGATGGTTGG GGATAATATT TCTTCCTCTG GCAATGAAGT CCTTGGGACA
    TCAATGGAGA AGCAGAACCT GGTAAACACT CAGTCACTTC GGGTTCCATT CAGCAGATCT
    CTTTCAGAGC CAGAACCCGG CTCCAGTGTC AGATTTGTTA GACGGTGGCC AACTTCAGTG
    GGGTCAGTGG AGTCTGATCA GCAGGGATAT TTCATCCGTG GCATTTTCTC TACTCTTTCC
    AATGGCTTCT CCAGGATGCT GAGTCTAAAA GGAGGGTCAA CCAGTGAGCC AGTAAGAGAA
    GATGTGAAAG GGCCACCTAC CCACCGTCTG TCTTGTGGCC AGTCGCCCTA CACCGACACA
    ACAACATGGG AGCGGAAGTA CTGCATCCTC ACAGACAGCC AGCTGGTGTT GCTCAACAAG
    GAGAAGGAGA TACCTGTGGA CGGAGGACAA GAACAGCAGG CGGATTCCAC CAAAGGGCGA
    TGCCTGCGGA GAACTGTCAG TGTTCCTTCC GAGGGTCAGT TTCCTGAATA CCCACCAGAG
    GGCGCCACTA AACTGGAGGT GCCAGCAGAA AGGTCCCCAC GCAGACGGAG TATCTCAGGG
    ACCACTACAT CTGAGAAACC CAACTCTATG GACACTGCGA ATACCTCACC CTTCAAAGTA
    CCAGGGTTCT TCAGCAAGCG CCTAAAAGGC TCCATCAAGA GGACCAAAAG CCAGTCAAAG
    CTTGACCGAA ACACGAGCTT TCGGCTTCCC TCTCTTCGCA ATACCGATGA CAGGTCTCGT
    GGGCTGCCTA AACTAAAAGA GTCCCGTTCT CATGAATCTT TGCTGAGCCC ATGCAGTGCC
    GTGGAATGTC TGGATCTCGG TAGAGGGGAG CCTGTGTCGG TAAAGCCACT CCATAGCAGC
    ATCCTTGGAC AAGACTTCTG CTTTGAGGTT ACCTACTTAA GTGGAAGTAA ATGCTTCAGT
    TGTAACTCTG CTTCAGAGAG AGATAAGTGG ATGGAAAACC TGCGCAGGAC AGTTCAACCA
    AATAAGGACA ATTGTAGACG AGCTGAAAAT GTTCTCCGTT TATGGATTAT TGAAGCCAAG
    GACCTTGCCC CTAAAAAGAA ATATTTCTGT GAACTGTGCC TTGATGATAC CCTCTTTGCT
    CGTACAACTA GCAAGACCAA AGCAGACAAT ATTTTCTGGG GTGAACATTT TGAGTTCTAC
    AGCCTTCCAC CTCTTCATAG CATCACAGTT CACATTTATA AAGATGTGGA AAAAAAGAAG
    AAAAAAGACA AGAATAATTA TGTAGGGCTG GTCAACATCC CCACTGCCAG TGTGACTGGT
    CGCCAGTTTG TAGAAAAGTG GTACCCAGTG AGCACACCTA CACCCAACAA AGGAAAGACA
    GGAGGACCTT CTATTCGGAT TAAGTCGCGT TTCCAAACCA TCACCATTCT GCCTATGGAG
    CAATACAAAG AGTTTGCAGA ATTTGTCACC AGCAACTATA CCATGTTATG TTCTGTTCTT
    GAACCAGTAA TCAGTGTGAG AAATAAAGAA GAGCTAGCCT GTGCCTTAGT GCACATTCTT
    CAAAGCACTG GAAGGGCCAA GGATTTTCTG ACGGACTTGG TGATGTCTGA GGTGGATCGT
    TGTGGAGAAC ATGATGTCTT GATCTTCAGA GAGAACACCA TTGCCACCAA ATCCATTGAG
    GAATACCTCA AGTTGGTGGG ACAACAGTAT CTTCACGATG CACTGGGGGA GTTCATCAAA
    GCGTTGTATG AGTCAGATGA AAACTGTGAA GTGGATCCCA GCAAATGTTC ATCCAGTGAA
    CTTCTGGACC ATCAGAGCAA CCTGAAAATG TGCTGTGAGC TGGCTTTCTG CAAGATCATC
    AACTCTTACT GTGTTTTTCC TCGTGAGCTG AAAGAAGTGT TTGCATCCTG GAAGCAGCAG
    TGCCTGAACC GCGGCAAGCA GGACATCAGC GAGAGGCTCA TCAGCGCCTC GCTGTTTCTC
    CGTTTTCTGT GCCCGGCCAT CATGTCTCCC AGTCTCTTCA ACCTCATGCA GGAGTATCCT
    GATGACCGCA CATCTCGGAC TCTGACTCTC ATTGCCAAGG TCATCCAGAA CCTGGCCAAC
    TTTGCCAAAT TTGGTAACAA GGAGGAATAC ATGGCATTCA TGAATGATTT TTTAGAACAT
    GAATGGGGTG GAATGAAGCG CTTTCTTTTG GAGATCTCTA ATCCAGACAC CATCTCAAAC
    ACCCCAGGCT TTGATGGTTA CATTGATCTG GGCCGAGAGC TTTCAGTTTT GCATTCCTTA
    CTATGGGAAG TAGTTTCCCA ACTTGATAAG GCAACCGTGG CAAAATTGGG ACCTCTCCCT
    CGTGTTCTTG CTGATATTAC CAAATCTCTG ACTAATCCTA CACCAATACA ACAGCAACTG
    AGACGCTTCA CTGAGCATAA CTCCAGTCCA AATGTCAGTG GAAGCCTCTC CTCTGGGCTG
    CAGAAAATAT TTGAAGACCC TACTGACAGT GATTTGCACA AACTAAAATC CCCAAGCCAG
    GACAACACAG ACAGTTATTT CAGAGGGAAG ACATTATTGC TGGTCCAGCA AGCCTCCTCC
    CAGAGCATGA CTTACTCGGA AAAGGATGAA AAGGAAAGTA GCCTCCCCAA TGGCCGCAGC
    ATCTCCCTCA TGGACCTTCA GGAGACGCAC GCAGCCCAAG CAGAGCATCC ATCTGTGATA
    CTCGACGTGC CCATGCGCTT GACCGGAAGC CAGCTCTCCA TAACTCAGGT GGCCAGCATC
    AAGCAACTGC GGGAGACCCA GAGCACCCCC CAGAGTGCAC CCCAAGTGCG AAGGCCCCTG
    CACCCAGCCT TGAACCAGCC AGGCAGCCTC CAGCCCCTGT CCTTCCAGAA CCCTGTCTAT
    CACCTCAGTA ACCCCATTCC TGCAATGCCA AAGGCCTCTG TGGACTCCAG TTTGGAGAAC
    CTAAGCACTG CCAGTTCCAG GAGCCAAAGT AACAGTGAAG ACTTCAAACT CAGCGGACCC
    AGCAACAGCA GCATGGAGGA CTTCACCAAA CGGAGCACGC AGAGTGAGGA CTTCTCCAGG
    CGCCACACCA TGCCCGACAG ACACATACCT CTCGCCCTGC CGCGGCAGAA CAGCACGGGG
    CAGACCCAGC TCCGGAAGAT GGACCCGGCG GGGCTGGGCG CCCGAGCCAA AGCCCCGCCG
    TCCCTGCCGC ACAGCGCCTC CCTGCGGAGC ACCGGGAGCA TGTCGGTGGC ATCAGCGGCT
    CTGGTGGCCG AGCCCCTGCA GAACGGGAGC CGGTCCCGAC AGCAGTCCTC TTCCTCCAGA
    GAGAGCCCGG TCCCCAAAGT GAGAGCCATC CAGAGGCAGC AGACCCAGCA GGTTCAGTCA
    CCTGTGGACT CCGCCACAAT GTCCCCAGTC GAGAGGACAG CAGCCTGGGT CCTGAACAAT
    GGGCAGTATG AAGAGGACGT GGAAGAGACT GAGCAAAATC AGGACGAAGC CAAGCATGCT
    GAGAAGTACG AACAGGAAAT CACCAAACTG AAGGAGCGCC TGCGCGTGTC CAGCCGGCGA
    CTCGAGGAGT ATGAGCGCCG GTTGCTAGTG CAGGAGCAGC AGATGCAGAA GCTGCTGCTG
    GAGTACAAGG CCCGGCTGGA GGACAGCGAG GAGCGGCTGC GCAGGCAGCA GGAGGAGAAG
    GACAGCCAGA TGAAGAGCAT CATCAGCAGG CTAATGGCTG TGGAAGAGGA ACTGAAGAAG
    GATCATGCTG AGATGCAGGC AGTTATTGAT GCAAAGCAAA AAATAATCGA TGCACAGGAA
    AAACGGATCG TGTCGCTGGA TTCGGCCAAC ACCAGACTGA TGAGTGCGCT GACCCAGGTG
    AAGGAACGGT ACAGCATGCA GGTCCGAAAT GGCATCTCCC CCACCAACCC CACCAAGCTT
    TCCATCACGG AGAATGGTGA ATTCAAAAAC AGCAGCTGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832634.1
    CDS285..4526
    Translation

    Target ORF information:

    RefSeq Version XM_024976866.1
    Organism Bos taurus(cattle)
    Definition Bos taurus RAS protein activator like 2 (RASAL2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976866.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGAGTCCT GGAAGATGAG CATCTTTAAG AGACAGACAT CACCGTGTCC TCCAAGCTAT 
    CGAAGATCTT TGTCAGAACC TGCTTATACA CACTCAGAGA TAGGAACAAG CTTAAAGAGT
    TATCCATGGC GTGGTCCAGT GAAAAGCAAG AGGAAGAAAA ATGAACAGGC TGTTTCCAGT
    CTAGGAAAGC CACTGGGTGC TTATCGGTGG CAAACACACC ACAGCTTAAG GTTGAAAGGG
    GACAAAAAGG AGCAATTGGA AAATAATAAA GGAGACTTGG ATGAACCACC ACAACAAGCA
    GTCCTGCCAA AGATGGTTGG GGATAATATT TCTTCCTCTG GCAATGAAGT CCTTGGGACA
    TCAATGGAGA AGCAGAACCT GGTAAACACT CAGTCACTTC GGGTTCCATT CAGCAGATCT
    CTTTCAGAGC CAGAACCCGG CTCCAGTGTC AGATTTGTTA GACGGTGGCC AACTTCAGTG
    GGGTCAGTGG AGTCTGATCA GCAGGGATAT TTCATCCGTG GCATTTTCTC TACTCTTTCC
    AATGGCTTCT CCAGGATGCT GAGTCTAAAA GGAGGGTCAA CCAGTGAGCC AGTAAGAGAA
    GATGTGAAAG GGCCACCTAC CCACCGTCTG TCTTGTGGCC AGTCGCCCTA CACCGACACA
    ACAACATGGG AGCGGAAGTA CTGCATCCTC ACAGACAGCC AGCTGGTGTT GCTCAACAAG
    GAGAAGGAGA TACCTGTGGA CGGAGGACAA GAACAGCAGG CGGATTCCAC CAAAGGGCGA
    TGCCTGCGGA GAACTGTCAG TGTTCCTTCC GAGGGTCAGT TTCCTGAATA CCCACCAGAG
    GGCGCCACTA AACTGGAGGT GCCAGCAGAA AGGTCCCCAC GCAGACGGAG TATCTCAGGG
    ACCACTACAT CTGAGAAACC CAACTCTATG GACACTGCGA ATACCTCACC CTTCAAAGTA
    CCAGGGTTCT TCAGCAAGCG CCTAAAAGGC TCCATCAAGA GGACCAAAAG CCAGTCAAAG
    CTTGACCGAA ACACGAGCTT TCGGCTTCCC TCTCTTCGCA ATACCGATGA CAGGTCTCGT
    GGGCTGCCTA AACTAAAAGA GTCCCGTTCT CATGAATCTT TGCTGAGCCC ATGCAGTGCC
    GTGGAATGTC TGGATCTCGG TAGAGGGGAG CCTGTGTCGG TAAAGCCACT CCATAGCAGC
    ATCCTTGGAC AAGACTTCTG CTTTGAGGTT ACCTACTTAA GTGGAAGTAA ATGCTTCAGT
    TGTAACTCTG CTTCAGAGAG AGATAAGTGG ATGGAAAACC TGCGCAGGAC AGTTCAACCA
    AATAAGGACA ATTGTAGACG AGCTGAAAAT GTTCTCCGTT TATGGATTAT TGAAGCCAAG
    GACCTTGCCC CTAAAAAGAA ATATTTCTGT GAACTGTGCC TTGATGATAC CCTCTTTGCT
    CGTACAACTA GCAAGACCAA AGCAGACAAT ATTTTCTGGG GTGAACATTT TGAGTTCTAC
    AGCCTTCCAC CTCTTCATAG CATCACAGTT CACATTTATA AAGATGTGGA AAAAAAGAAG
    AAAAAAGACA AGAATAATTA TGTAGGGCTG GTCAACATCC CCACTGCCAG TGTGACTGGT
    CGCCAGTTTG TAGAAAAGTG GTACCCAGTG AGCACACCTA CACCCAACAA AGGAAAGACA
    GGAGGACCTT CTATTCGGAT TAAGTCGCGT TTCCAAACCA TCACCATTCT GCCTATGGAG
    CAATACAAAG AGTTTGCAGA ATTTGTCACC AGCAACTATA CCATGTTATG TTCTGTTCTT
    GAACCAGTAA TCAGTGTGAG AAATAAAGAA GAGCTAGCCT GTGCCTTAGT GCACATTCTT
    CAAAGCACTG GAAGGGCCAA GGATTTTCTG ACGGACTTGG TGATGTCTGA GGTGGATCGT
    TGTGGAGAAC ATGATGTCTT GATCTTCAGA GAGAACACCA TTGCCACCAA ATCCATTGAG
    GAATACCTCA AGTTGGTGGG ACAACAGTAT CTTCACGATG CACTGGGGGA GTTCATCAAA
    GCGTTGTATG AGTCAGATGA AAACTGTGAA GTGGATCCCA GCAAATGTTC ATCCAGTGAA
    CTTCTGGACC ATCAGAGCAA CCTGAAAATG TGCTGTGAGC TGGCTTTCTG CAAGATCATC
    AACTCTTACT GTGTTTTTCC TCGTGAGCTG AAAGAAGTGT TTGCATCCTG GAAGCAGCAG
    TGCCTGAACC GCGGCAAGCA GGACATCAGC GAGAGGCTCA TCAGCGCCTC GCTGTTTCTC
    CGTTTTCTGT GCCCGGCCAT CATGTCTCCC AGTCTCTTCA ACCTCATGCA GGAGTATCCT
    GATGACCGCA CATCTCGGAC TCTGACTCTC ATTGCCAAGG TCATCCAGAA CCTGGCCAAC
    TTTGCCAAAT TTGGTAACAA GGAGGAATAC ATGGCATTCA TGAATGATTT TTTAGAACAT
    GAATGGGGTG GAATGAAGCG CTTTCTTTTG GAGATCTCTA ATCCAGACAC CATCTCAAAC
    ACCCCAGGCT TTGATGGTTA CATTGATCTG GGCCGAGAGC TTTCAGTTTT GCATTCCTTA
    CTATGGGAAG TAGTTTCCCA ACTTGATAAG GCAACCGTGG CAAAATTGGG ACCTCTCCCT
    CGTGTTCTTG CTGATATTAC CAAATCTCTG ACTAATCCTA CACCAATACA ACAGCAACTG
    AGACGCTTCA CTGAGCATAA CTCCAGTCCA AATGTCAGTG GAAGCCTCTC CTCTGGGCTG
    CAGAAAATAT TTGAAGACCC TACTGACAGT GATTTGCACA AACTAAAATC CCCAAGCCAG
    GACAACACAG ACAGTTATTT CAGAGGGAAG ACATTATTGC TGGTCCAGCA AGCCTCCTCC
    CAGAGCATGA CTTACTCGGA AAAGGATGAA AAGGAAAGTA GCCTCCCCAA TGGCCGCAGC
    ATCTCCCTCA TGGACCTTCA GGAGACGCAC GCAGCCCAAG CAGAGCATCC ATCTGTGATA
    CTCGACGTGC CCATGCGCTT GACCGGAAGC CAGCTCTCCA TAACTCAGGT GGCCAGCATC
    AAGCAACTGC GGGAGACCCA GAGCACCCCC CAGAGTGCAC CCCAAGTGCG AAGGCCCCTG
    CACCCAGCCT TGAACCAGCC AGGCAGCCTC CAGCCCCTGT CCTTCCAGAA CCCTGTCTAT
    CACCTCAGTA ACCCCATTCC TGCAATGCCA AAGGCCTCTG TGGACTCCAG TTTGGAGAAC
    CTAAGCACTG CCAGTTCCAG GAGCCAAAGT AACAGTGAAG ACTTCAAACT CAGCGGACCC
    AGCAACAGCA GCATGGAGGA CTTCACCAAA CGGAGCACGC AGAGTGAGGA CTTCTCCAGG
    CGCCACACCA TGCCCGACAG ACACATACCT CTCGCCCTGC CGCGGCAGAA CAGCACGGGG
    CAGACCCAGC TCCGGAAGAT GGACCCGGCG GGGCTGGGCG CCCGAGCCAA AGCCCCGCCG
    TCCCTGCCGC ACAGCGCCTC CCTGCGGAGC ACCGGGAGCA TGTCGGTGGC ATCAGCGGCT
    CTGGTGGCCG AGCCCCTGCA GAACGGGAGC CGGTCCCGAC AGCAGTCCTC TTCCTCCAGA
    GAGAGCCCGG TCCCCAAAGT GAGAGCCATC CAGAGGCAGC AGACCCAGCA GGTTCAGTCA
    CCTGTGGACT CCGCCACAAT GTCCCCAGTC GAGAGGACAG CAGCCTGGGT CCTGAACAAT
    GGGCAGTATG AAGAGGACGT GGAAGAGACT GAGCAAAATC AGGACGAAGC CAAGCATGCT
    GAGAAGTACG AACAGGAAAT CACCAAACTG AAGGAGCGCC TGCGCGTGTC CAGCCGGCGA
    CTCGAGGAGT ATGAGCGCCG GTTGCTAGTG CAGGAGCAGC AGATGCAGAA GCTGCTGCTG
    GAGTACAAGG CCCGGCTGGA GGACAGCGAG GAGCGGCTGC GCAGGCAGCA GGAGGAGAAG
    GACAGCCAGA TGAAGAGCAT CATCAGCAGG CTAATGGCTG TGGAAGAGGA ACTGAAGAAG
    GATCATGCTG AGATGCAGGC AGTTATTGAT GCAAAGCAAA AAATAATCGA TGCACAGGAA
    AAACGGATCG TGTCGCTGGA TTCGGCCAAC ACCAGACTGA TGAGTGCGCT GACCCAGGTG
    AAGGAACGGT ACAGCATGCA GGTCCGAAAT GGCATCTCCC CCACCAACCC CACCAAGCTT
    TCCATCACGG AGAATGGTGA ATTCAAAAAC AGCAGCTGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa224038
    Clone ID Related Accession (Same CDS sequence) XM_005217241.4
    Accession Version XM_005217241.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3873bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ras GTPase-activating protein nGAP isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005217241.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCTCT CCCTGTCGTC CGGAGGAGCC GCGGAGGCGC TGTCCTGGCC AGAGATGTTC 
    CCGGGATTGG AGTCCGACTC GCCGCTCCCC CCGGAGGAGC TGGACGCGGT TGTCCCGGTC
    AGTGGGGCCA TGGCCGGTGG CATGTTGGAT CGGATTCTTC TGGAGTCCGT GTGCCAGCAA
    CAGAGCTGGG TCCGCGTGTA CGATGTGAAA GGGCCACCTA CCCACCGTCT GTCTTGTGGC
    CAGTCGCCCT ACACCGACAC AACAACATGG GAGCGGAAGT ACTGCATCCT CACAGACAGC
    CAGCTGGTGT TGCTCAACAA GGAGAAGGAG ATACCTGTGG ACGGAGGACA AGAACAGCAG
    GCGGATTCCA CCAAAGGGCG ATGCCTGCGG AGAACTGTCA GTGTTCCTTC CGAGGGTCAG
    TTTCCTGAAT ACCCACCAGA GGGCGCCACT AAACTGGAGG TGCCAGCAGA AAGGTCCCCA
    CGCAGACGGA GTATCTCAGG GACCACTACA TCTGAGAAAC CCAACTCTAT GGACACTGCG
    AATACCTCAC CCTTCAAAGT ACCAGGGTTC TTCAGCAAGC GCCTAAAAGG CTCCATCAAG
    AGGACCAAAA GCCAGTCAAA GCTTGACCGA AACACGAGCT TTCGGCTTCC CTCTCTTCGC
    AATACCGATG ACAGGTCTCG TGGGCTGCCT AAACTAAAAG AGTCCCGTTC TCATGAATCT
    TTGCTGAGCC CATGCAGTGC CGTGGAATGT CTGGATCTCG GTAGAGGGGA GCCTGTGTCG
    GTAAAGCCAC TCCATAGCAG CATCCTTGGA CAAGACTTCT GCTTTGAGGT TACCTACTTA
    AGTGGAAGTA AATGCTTCAG TTGTAACTCT GCTTCAGAGA GAGATAAGTG GATGGAAAAC
    CTGCGCAGGA CAGTTCAACC AAATAAGGAC AATTGTAGAC GAGCTGAAAA TGTTCTCCGT
    TTATGGATTA TTGAAGCCAA GGACCTTGCC CCTAAAAAGA AATATTTCTG TGAACTGTGC
    CTTGATGATA CCCTCTTTGC TCGTACAACT AGCAAGACCA AAGCAGACAA TATTTTCTGG
    GGTGAACATT TTGAGTTCTA CAGCCTTCCA CCTCTTCATA GCATCACAGT TCACATTTAT
    AAAGATGTGG AAAAAAAGAA GAAAAAAGAC AAGAATAATT ATGTAGGGCT GGTCAACATC
    CCCACTGCCA GTGTGACTGG TCGCCAGTTT GTAGAAAAGT GGTACCCAGT GAGCACACCT
    ACACCCAACA AAGGAAAGAC AGGAGGACCT TCTATTCGGA TTAAGTCGCG TTTCCAAACC
    ATCACCATTC TGCCTATGGA GCAATACAAA GAGTTTGCAG AATTTGTCAC CAGCAACTAT
    ACCATGTTAT GTTCTGTTCT TGAACCAGTA ATCAGTGTGA GAAATAAAGA AGAGCTAGCC
    TGTGCCTTAG TGCACATTCT TCAAAGCACT GGAAGGGCCA AGGATTTTCT GACGGACTTG
    GTGATGTCTG AGGTGGATCG TTGTGGAGAA CATGATGTCT TGATCTTCAG AGAGAACACC
    ATTGCCACCA AATCCATTGA GGAATACCTC AAGTTGGTGG GACAACAGTA TCTTCACGAT
    GCACTGGGGG AGTTCATCAA AGCGTTGTAT GAGTCAGATG AAAACTGTGA AGTGGATCCC
    AGCAAATGTT CATCCAGTGA ACTTCTGGAC CATCAGAGCA ACCTGAAAAT GTGCTGTGAG
    CTGGCTTTCT GCAAGATCAT CAACTCTTAC TGTGTTTTTC CTCGTGAGCT GAAAGAAGTG
    TTTGCATCCT GGAAGCAGCA GTGCCTGAAC CGCGGCAAGC AGGACATCAG CGAGAGGCTC
    ATCAGCGCCT CGCTGTTTCT CCGTTTTCTG TGCCCGGCCA TCATGTCTCC CAGTCTCTTC
    AACCTCATGC AGGAGTATCC TGATGACCGC ACATCTCGGA CTCTGACTCT CATTGCCAAG
    GTCATCCAGA ACCTGGCCAA CTTTGCCAAA TTTGGTAACA AGGAGGAATA CATGGCATTC
    ATGAATGATT TTTTAGAACA TGAATGGGGT GGAATGAAGC GCTTTCTTTT GGAGATCTCT
    AATCCAGACA CCATCTCAAA CACCCCAGGC TTTGATGGTT ACATTGATCT GGGCCGAGAG
    CTTTCAGTTT TGCATTCCTT ACTATGGGAA GTAGTTTCCC AACTTGATAA GGGTGAAAAT
    TCCTTCCTAC AGGCAACCGT GGCAAAATTG GGACCTCTCC CTCGTGTTCT TGCTGATATT
    ACCAAATCTC TGACTAATCC TACACCAATA CAACAGCAAC TGAGACGCTT CACTGAGCAT
    AACTCCAGTC CAAATGTCAG TGGAAGCCTC TCCTCTGGGC TGCAGAAAAT ATTTGAAGAC
    CCTACTGACA GTGATTTGCA CAAACTAAAA TCCCCAAGCC AGGACAACAC AGACAGTTAT
    TTCAGAGGGA AGACATTATT GCTGGTCCAG CAAGCCTCCT CCCAGAGCAT GACTTACTCG
    GAAAAGGATG AAAAGGAAAG TAGCCTCCCC AATGGCCGCA GCATCTCCCT CATGGACCTT
    CAGGAGACGC ACGCAGCCCA AGCAGAGCAT CCATCTGTGA TACTCGACGT GCCCATGCGC
    TTGACCGGAA GCCAGCTCTC CATAACTCAG GTGGCCAGCA TCAAGCAACT GCGGGAGACC
    CAGAGCACCC CCCAGAGTGC ACCCCAAGTG CGAAGGCCCC TGCACCCAGC CTTGAACCAG
    CCAGGCAGCC TCCAGCCCCT GTCCTTCCAG AACCCTGTCT ATCACCTCAG TAACCCCATT
    CCTGCAATGC CAAAGGCCTC TGTGGACTCC AGTTTGGAGA ACCTAAGCAC TGCCAGTTCC
    AGGAGCCAAA GTAACAGTGA AGACTTCAAA CTCAGCGGAC CCAGCAACAG CAGCATGGAG
    GACTTCACCA AACGGAGCAC GCAGAGTGAG GACTTCTCCA GGCGCCACAC CATGCCCGAC
    AGACACATAC CTCTCGCCCT GCCGCGGCAG AACAGCACGG GGCAGACCCA GCTCCGGAAG
    ATGGACCCGG CGGGGCTGGG CGCCCGAGCC AAAGCCCCGC CGTCCCTGCC GCACAGCGCC
    TCCCTGCGGA GCACCGGGAG CATGTCGGTG GCATCAGCGG CTCTGGTGGC CGAGCCCCTG
    CAGAACGGGA GCCGGTCCCG ACAGCAGTCC TCTTCCTCCA GAGAGAGCCC GGTCCCCAAA
    GTGAGAGCCA TCCAGAGGCA GCAGACCCAG CAGGTTCAGT CACCTGTGGA CTCCGCCACA
    ATGTCCCCAG TCGAGAGGAC AGCAGCCTGG GTCCTGAACA ATGGGCAGTA TGAAGAGGAC
    GTGGAAGAGA CTGAGCAAAA TCAGGACGAA GCCAAGCATG CTGAGAAGTA CGAACAGGAA
    ATCACCAAAC TGAAGGAGCG CCTGCGCGTG TCCAGCCGGC GACTCGAGGA GTATGAGCGC
    CGGTTGCTAG TGCAGGAGCA GCAGATGCAG AAGCTGCTGC TGGAGTACAA GGCCCGGCTG
    GAGGACAGCG AGGAGCGGCT GCGCAGGCAG CAGGAGGAGA AGGACAGCCA GATGAAGAGC
    ATCATCAGCA GGCTAATGGC TGTGGAAGAG GAACTGAAGA AGGATCATGC TGAGATGCAG
    GCAGTTATTG ATGCAAAGCA AAAAATAATC GATGCACAGG TCATAAATGG AGATGAGATT
    ATGCAAACAG GAAAAACGGA TCGTGTCGCT GGATTCGGCC AACACCAGAC TGATGAGTGC
    GCTGACCCAG GTGAAGGAAC GGTACAGCAT GCAGGTCCGA AATGGCATCT CCCCCACCAA
    CCCCACCAAG CTTTCCATCA CGGAGAATGG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005217298.1
    CDS696..4568
    Translation

    Target ORF information:

    RefSeq Version XM_005217241.4
    Organism Bos taurus(cattle)
    Definition Bos taurus RAS protein activator like 2 (RASAL2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005217241.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCTCT CCCTGTCGTC CGGAGGAGCC GCGGAGGCGC TGTCCTGGCC AGAGATGTTC 
    CCGGGATTGG AGTCCGACTC GCCGCTCCCC CCGGAGGAGC TGGACGCGGT TGTCCCGGTC
    AGTGGGGCCA TGGCCGGTGG CATGTTGGAT CGGATTCTTC TGGAGTCCGT GTGCCAGCAA
    CAGAGCTGGG TCCGCGTGTA CGATGTGAAA GGGCCACCTA CCCACCGTCT GTCTTGTGGC
    CAGTCGCCCT ACACCGACAC AACAACATGG GAGCGGAAGT ACTGCATCCT CACAGACAGC
    CAGCTGGTGT TGCTCAACAA GGAGAAGGAG ATACCTGTGG ACGGAGGACA AGAACAGCAG
    GCGGATTCCA CCAAAGGGCG ATGCCTGCGG AGAACTGTCA GTGTTCCTTC CGAGGGTCAG
    TTTCCTGAAT ACCCACCAGA GGGCGCCACT AAACTGGAGG TGCCAGCAGA AAGGTCCCCA
    CGCAGACGGA GTATCTCAGG GACCACTACA TCTGAGAAAC CCAACTCTAT GGACACTGCG
    AATACCTCAC CCTTCAAAGT ACCAGGGTTC TTCAGCAAGC GCCTAAAAGG CTCCATCAAG
    AGGACCAAAA GCCAGTCAAA GCTTGACCGA AACACGAGCT TTCGGCTTCC CTCTCTTCGC
    AATACCGATG ACAGGTCTCG TGGGCTGCCT AAACTAAAAG AGTCCCGTTC TCATGAATCT
    TTGCTGAGCC CATGCAGTGC CGTGGAATGT CTGGATCTCG GTAGAGGGGA GCCTGTGTCG
    GTAAAGCCAC TCCATAGCAG CATCCTTGGA CAAGACTTCT GCTTTGAGGT TACCTACTTA
    AGTGGAAGTA AATGCTTCAG TTGTAACTCT GCTTCAGAGA GAGATAAGTG GATGGAAAAC
    CTGCGCAGGA CAGTTCAACC AAATAAGGAC AATTGTAGAC GAGCTGAAAA TGTTCTCCGT
    TTATGGATTA TTGAAGCCAA GGACCTTGCC CCTAAAAAGA AATATTTCTG TGAACTGTGC
    CTTGATGATA CCCTCTTTGC TCGTACAACT AGCAAGACCA AAGCAGACAA TATTTTCTGG
    GGTGAACATT TTGAGTTCTA CAGCCTTCCA CCTCTTCATA GCATCACAGT TCACATTTAT
    AAAGATGTGG AAAAAAAGAA GAAAAAAGAC AAGAATAATT ATGTAGGGCT GGTCAACATC
    CCCACTGCCA GTGTGACTGG TCGCCAGTTT GTAGAAAAGT GGTACCCAGT GAGCACACCT
    ACACCCAACA AAGGAAAGAC AGGAGGACCT TCTATTCGGA TTAAGTCGCG TTTCCAAACC
    ATCACCATTC TGCCTATGGA GCAATACAAA GAGTTTGCAG AATTTGTCAC CAGCAACTAT
    ACCATGTTAT GTTCTGTTCT TGAACCAGTA ATCAGTGTGA GAAATAAAGA AGAGCTAGCC
    TGTGCCTTAG TGCACATTCT TCAAAGCACT GGAAGGGCCA AGGATTTTCT GACGGACTTG
    GTGATGTCTG AGGTGGATCG TTGTGGAGAA CATGATGTCT TGATCTTCAG AGAGAACACC
    ATTGCCACCA AATCCATTGA GGAATACCTC AAGTTGGTGG GACAACAGTA TCTTCACGAT
    GCACTGGGGG AGTTCATCAA AGCGTTGTAT GAGTCAGATG AAAACTGTGA AGTGGATCCC
    AGCAAATGTT CATCCAGTGA ACTTCTGGAC CATCAGAGCA ACCTGAAAAT GTGCTGTGAG
    CTGGCTTTCT GCAAGATCAT CAACTCTTAC TGTGTTTTTC CTCGTGAGCT GAAAGAAGTG
    TTTGCATCCT GGAAGCAGCA GTGCCTGAAC CGCGGCAAGC AGGACATCAG CGAGAGGCTC
    ATCAGCGCCT CGCTGTTTCT CCGTTTTCTG TGCCCGGCCA TCATGTCTCC CAGTCTCTTC
    AACCTCATGC AGGAGTATCC TGATGACCGC ACATCTCGGA CTCTGACTCT CATTGCCAAG
    GTCATCCAGA ACCTGGCCAA CTTTGCCAAA TTTGGTAACA AGGAGGAATA CATGGCATTC
    ATGAATGATT TTTTAGAACA TGAATGGGGT GGAATGAAGC GCTTTCTTTT GGAGATCTCT
    AATCCAGACA CCATCTCAAA CACCCCAGGC TTTGATGGTT ACATTGATCT GGGCCGAGAG
    CTTTCAGTTT TGCATTCCTT ACTATGGGAA GTAGTTTCCC AACTTGATAA GGGTGAAAAT
    TCCTTCCTAC AGGCAACCGT GGCAAAATTG GGACCTCTCC CTCGTGTTCT TGCTGATATT
    ACCAAATCTC TGACTAATCC TACACCAATA CAACAGCAAC TGAGACGCTT CACTGAGCAT
    AACTCCAGTC CAAATGTCAG TGGAAGCCTC TCCTCTGGGC TGCAGAAAAT ATTTGAAGAC
    CCTACTGACA GTGATTTGCA CAAACTAAAA TCCCCAAGCC AGGACAACAC AGACAGTTAT
    TTCAGAGGGA AGACATTATT GCTGGTCCAG CAAGCCTCCT CCCAGAGCAT GACTTACTCG
    GAAAAGGATG AAAAGGAAAG TAGCCTCCCC AATGGCCGCA GCATCTCCCT CATGGACCTT
    CAGGAGACGC ACGCAGCCCA AGCAGAGCAT CCATCTGTGA TACTCGACGT GCCCATGCGC
    TTGACCGGAA GCCAGCTCTC CATAACTCAG GTGGCCAGCA TCAAGCAACT GCGGGAGACC
    CAGAGCACCC CCCAGAGTGC ACCCCAAGTG CGAAGGCCCC TGCACCCAGC CTTGAACCAG
    CCAGGCAGCC TCCAGCCCCT GTCCTTCCAG AACCCTGTCT ATCACCTCAG TAACCCCATT
    CCTGCAATGC CAAAGGCCTC TGTGGACTCC AGTTTGGAGA ACCTAAGCAC TGCCAGTTCC
    AGGAGCCAAA GTAACAGTGA AGACTTCAAA CTCAGCGGAC CCAGCAACAG CAGCATGGAG
    GACTTCACCA AACGGAGCAC GCAGAGTGAG GACTTCTCCA GGCGCCACAC CATGCCCGAC
    AGACACATAC CTCTCGCCCT GCCGCGGCAG AACAGCACGG GGCAGACCCA GCTCCGGAAG
    ATGGACCCGG CGGGGCTGGG CGCCCGAGCC AAAGCCCCGC CGTCCCTGCC GCACAGCGCC
    TCCCTGCGGA GCACCGGGAG CATGTCGGTG GCATCAGCGG CTCTGGTGGC CGAGCCCCTG
    CAGAACGGGA GCCGGTCCCG ACAGCAGTCC TCTTCCTCCA GAGAGAGCCC GGTCCCCAAA
    GTGAGAGCCA TCCAGAGGCA GCAGACCCAG CAGGTTCAGT CACCTGTGGA CTCCGCCACA
    ATGTCCCCAG TCGAGAGGAC AGCAGCCTGG GTCCTGAACA ATGGGCAGTA TGAAGAGGAC
    GTGGAAGAGA CTGAGCAAAA TCAGGACGAA GCCAAGCATG CTGAGAAGTA CGAACAGGAA
    ATCACCAAAC TGAAGGAGCG CCTGCGCGTG TCCAGCCGGC GACTCGAGGA GTATGAGCGC
    CGGTTGCTAG TGCAGGAGCA GCAGATGCAG AAGCTGCTGC TGGAGTACAA GGCCCGGCTG
    GAGGACAGCG AGGAGCGGCT GCGCAGGCAG CAGGAGGAGA AGGACAGCCA GATGAAGAGC
    ATCATCAGCA GGCTAATGGC TGTGGAAGAG GAACTGAAGA AGGATCATGC TGAGATGCAG
    GCAGTTATTG ATGCAAAGCA AAAAATAATC GATGCACAGG TCATAAATGG AGATGAGATT
    ATGCAAACAG GAAAAACGGA TCGTGTCGCT GGATTCGGCC AACACCAGAC TGATGAGTGC
    GCTGACCCAG GTGAAGGAAC GGTACAGCAT GCAGGTCCGA AATGGCATCT CCCCCACCAA
    CCCCACCAAG CTTTCCATCA CGGAGAATGG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa224039
    Clone ID Related Accession (Same CDS sequence) XM_002694157.6
    Accession Version XM_002694157.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3864bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ras GTPase-activating protein nGAP isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002694157.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCTCT CCCTGTCGTC CGGAGGAGCC GCGGAGGCGC TGTCCTGGCC AGAGATGTTC 
    CCGGGATTGG AGTCCGACTC GCCGCTCCCC CCGGAGGAGC TGGACGCGGT TGTCCCGGTC
    AGTGGGGCCA TGGCCGGTGG CATGTTGGAT CGGATTCTTC TGGAGTCCGT GTGCCAGCAA
    CAGAGCTGGG TCCGCGTGTA CGATGTGAAA GGGCCACCTA CCCACCGTCT GTCTTGTGGC
    CAGTCGCCCT ACACCGACAC AACAACATGG GAGCGGAAGT ACTGCATCCT CACAGACAGC
    CAGCTGGTGT TGCTCAACAA GGAGAAGGAG ATACCTGTGG ACGGAGGACA AGAACAGCAG
    GCGGATTCCA CCAAAGGGCG ATGCCTGCGG AGAACTGTCA GTGTTCCTTC CGAGGGTCAG
    TTTCCTGAAT ACCCACCAGA GGGCGCCACT AAACTGGAGG TGCCAGCAGA AAGGTCCCCA
    CGCAGACGGA GTATCTCAGG GACCACTACA TCTGAGAAAC CCAACTCTAT GGACACTGCG
    AATACCTCAC CCTTCAAAGT ACCAGGGTTC TTCAGCAAGC GCCTAAAAGG CTCCATCAAG
    AGGACCAAAA GCCAGTCAAA GCTTGACCGA AACACGAGCT TTCGGCTTCC CTCTCTTCGC
    AATACCGATG ACAGGTCTCG TGGGCTGCCT AAACTAAAAG AGTCCCGTTC TCATGAATCT
    TTGCTGAGCC CATGCAGTGC CGTGGAATGT CTGGATCTCG GTAGAGGGGA GCCTGTGTCG
    GTAAAGCCAC TCCATAGCAG CATCCTTGGA CAAGACTTCT GCTTTGAGGT TACCTACTTA
    AGTGGAAGTA AATGCTTCAG TTGTAACTCT GCTTCAGAGA GAGATAAGTG GATGGAAAAC
    CTGCGCAGGA CAGTTCAACC AAATAAGGAC AATTGTAGAC GAGCTGAAAA TGTTCTCCGT
    TTATGGATTA TTGAAGCCAA GGACCTTGCC CCTAAAAAGA AATATTTCTG TGAACTGTGC
    CTTGATGATA CCCTCTTTGC TCGTACAACT AGCAAGACCA AAGCAGACAA TATTTTCTGG
    GGTGAACATT TTGAGTTCTA CAGCCTTCCA CCTCTTCATA GCATCACAGT TCACATTTAT
    AAAGATGTGG AAAAAAAGAA GAAAAAAGAC AAGAATAATT ATGTAGGGCT GGTCAACATC
    CCCACTGCCA GTGTGACTGG TCGCCAGTTT GTAGAAAAGT GGTACCCAGT GAGCACACCT
    ACACCCAACA AAGGAAAGAC AGGAGGACCT TCTATTCGGA TTAAGTCGCG TTTCCAAACC
    ATCACCATTC TGCCTATGGA GCAATACAAA GAGTTTGCAG AATTTGTCAC CAGCAACTAT
    ACCATGTTAT GTTCTGTTCT TGAACCAGTA ATCAGTGTGA GAAATAAAGA AGAGCTAGCC
    TGTGCCTTAG TGCACATTCT TCAAAGCACT GGAAGGGCCA AGGATTTTCT GACGGACTTG
    GTGATGTCTG AGGTGGATCG TTGTGGAGAA CATGATGTCT TGATCTTCAG AGAGAACACC
    ATTGCCACCA AATCCATTGA GGAATACCTC AAGTTGGTGG GACAACAGTA TCTTCACGAT
    GCACTGGGGG AGTTCATCAA AGCGTTGTAT GAGTCAGATG AAAACTGTGA AGTGGATCCC
    AGCAAATGTT CATCCAGTGA ACTTCTGGAC CATCAGAGCA ACCTGAAAAT GTGCTGTGAG
    CTGGCTTTCT GCAAGATCAT CAACTCTTAC TGTGTTTTTC CTCGTGAGCT GAAAGAAGTG
    TTTGCATCCT GGAAGCAGCA GTGCCTGAAC CGCGGCAAGC AGGACATCAG CGAGAGGCTC
    ATCAGCGCCT CGCTGTTTCT CCGTTTTCTG TGCCCGGCCA TCATGTCTCC CAGTCTCTTC
    AACCTCATGC AGGAGTATCC TGATGACCGC ACATCTCGGA CTCTGACTCT CATTGCCAAG
    GTCATCCAGA ACCTGGCCAA CTTTGCCAAA TTTGGTAACA AGGAGGAATA CATGGCATTC
    ATGAATGATT TTTTAGAACA TGAATGGGGT GGAATGAAGC GCTTTCTTTT GGAGATCTCT
    AATCCAGACA CCATCTCAAA CACCCCAGGC TTTGATGGTT ACATTGATCT GGGCCGAGAG
    CTTTCAGTTT TGCATTCCTT ACTATGGGAA GTAGTTTCCC AACTTGATAA GGGTGAAAAT
    TCCTTCCTAC AGGCAACCGT GGCAAAATTG GGACCTCTCC CTCGTGTTCT TGCTGATATT
    ACCAAATCTC TGACTAATCC TACACCAATA CAACAGCAAC TGAGACGCTT CACTGAGCAT
    AACTCCAGTC CAAATGTCAG TGGAAGCCTC TCCTCTGGGC TGCAGAAAAT ATTTGAAGAC
    CCTACTGACA GTGATTTGCA CAAACTAAAA TCCCCAAGCC AGGACAACAC AGACAGTTAT
    TTCAGAGGGA AGACATTATT GCTGGTCCAG CAAGCCTCCT CCCAGAGCAT GACTTACTCG
    GAAAAGGATG AAAAGGAAAG TAGCCTCCCC AATGGCCGCA GCATCTCCCT CATGGACCTT
    CAGGAGACGC ACGCAGCCCA AGCAGAGCAT CCATCTGTGA TACTCGACGT GCCCATGCGC
    TTGACCGGAA GCCAGCTCTC CATAACTCAG GTGGCCAGCA TCAAGCAACT GCGGGAGACC
    CAGAGCACCC CCCAGAGTGC ACCCCAAGTG CGAAGGCCCC TGCACCCAGC CTTGAACCAG
    CCAGGCAGCC TCCAGCCCCT GTCCTTCCAG AACCCTGTCT ATCACCTCAG TAACCCCATT
    CCTGCAATGC CAAAGGCCTC TGTGGACTCC AGTTTGGAGA ACCTAAGCAC TGCCAGTTCC
    AGGAGCCAAA GTAACAGTGA AGACTTCAAA CTCAGCGGAC CCAGCAACAG CAGCATGGAG
    GACTTCACCA AACGGAGCAC GCAGAGTGAG GACTTCTCCA GGCGCCACAC CATGCCCGAC
    AGACACATAC CTCTCGCCCT GCCGCGGCAG AACAGCACGG GGCAGACCCA GCTCCGGAAG
    ATGGACCCGG CGGGGCTGGG CGCCCGAGCC AAAGCCCCGC CGTCCCTGCC GCACAGCGCC
    TCCCTGCGGA GCACCGGGAG CATGTCGGTG GCATCAGCGG CTCTGGTGGC CGAGCCCCTG
    CAGAACGGGA GCCGGTCCCG ACAGCAGTCC TCTTCCTCCA GAGAGAGCCC GGTCCCCAAA
    GTGAGAGCCA TCCAGAGGCA GCAGACCCAG CAGGTTCAGT CACCTGTGGA CTCCGCCACA
    ATGTCCCCAG TCGAGAGGAC AGCAGCCTGG GTCCTGAACA ATGGGCAGTA TGAAGAGGAC
    GTGGAAGAGA CTGAGCAAAA TCAGGACGAA GCCAAGCATG CTGAGAAGTA CGAACAGGAA
    ATCACCAAAC TGAAGGAGCG CCTGCGCGTG TCCAGCCGGC GACTCGAGGA GTATGAGCGC
    CGGTTGCTAG TGCAGGAGCA GCAGATGCAG AAGCTGCTGC TGGAGTACAA GGCCCGGCTG
    GAGGACAGCG AGGAGCGGCT GCGCAGGCAG CAGGAGGAGA AGGACAGCCA GATGAAGAGC
    ATCATCAGCA GGCTAATGGC TGTGGAAGAG GAACTGAAGA AGGATCATGC TGAGATGCAG
    GCAGTTATTG ATGCAAAGCA AAAAATAATC GATGCACAGG AAAAACGGAT CGTGTCGCTG
    GATTCGGCCA ACACCAGACT GATGAGTGCG CTGACCCAGG TGAAGGAACG GTACAGCATG
    CAGGTCCGAA ATGGCATCTC CCCCACCAAC CCCACCAAGC TTTCCATCAC GGAGAATGGT
    GAATTCAAAA ACAGCAGCTG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002694203.1
    CDS696..4559
    Translation

    Target ORF information:

    RefSeq Version XM_002694157.6
    Organism Bos taurus(cattle)
    Definition Bos taurus RAS protein activator like 2 (RASAL2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002694157.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCTCT CCCTGTCGTC CGGAGGAGCC GCGGAGGCGC TGTCCTGGCC AGAGATGTTC 
    CCGGGATTGG AGTCCGACTC GCCGCTCCCC CCGGAGGAGC TGGACGCGGT TGTCCCGGTC
    AGTGGGGCCA TGGCCGGTGG CATGTTGGAT CGGATTCTTC TGGAGTCCGT GTGCCAGCAA
    CAGAGCTGGG TCCGCGTGTA CGATGTGAAA GGGCCACCTA CCCACCGTCT GTCTTGTGGC
    CAGTCGCCCT ACACCGACAC AACAACATGG GAGCGGAAGT ACTGCATCCT CACAGACAGC
    CAGCTGGTGT TGCTCAACAA GGAGAAGGAG ATACCTGTGG ACGGAGGACA AGAACAGCAG
    GCGGATTCCA CCAAAGGGCG ATGCCTGCGG AGAACTGTCA GTGTTCCTTC CGAGGGTCAG
    TTTCCTGAAT ACCCACCAGA GGGCGCCACT AAACTGGAGG TGCCAGCAGA AAGGTCCCCA
    CGCAGACGGA GTATCTCAGG GACCACTACA TCTGAGAAAC CCAACTCTAT GGACACTGCG
    AATACCTCAC CCTTCAAAGT ACCAGGGTTC TTCAGCAAGC GCCTAAAAGG CTCCATCAAG
    AGGACCAAAA GCCAGTCAAA GCTTGACCGA AACACGAGCT TTCGGCTTCC CTCTCTTCGC
    AATACCGATG ACAGGTCTCG TGGGCTGCCT AAACTAAAAG AGTCCCGTTC TCATGAATCT
    TTGCTGAGCC CATGCAGTGC CGTGGAATGT CTGGATCTCG GTAGAGGGGA GCCTGTGTCG
    GTAAAGCCAC TCCATAGCAG CATCCTTGGA CAAGACTTCT GCTTTGAGGT TACCTACTTA
    AGTGGAAGTA AATGCTTCAG TTGTAACTCT GCTTCAGAGA GAGATAAGTG GATGGAAAAC
    CTGCGCAGGA CAGTTCAACC AAATAAGGAC AATTGTAGAC GAGCTGAAAA TGTTCTCCGT
    TTATGGATTA TTGAAGCCAA GGACCTTGCC CCTAAAAAGA AATATTTCTG TGAACTGTGC
    CTTGATGATA CCCTCTTTGC TCGTACAACT AGCAAGACCA AAGCAGACAA TATTTTCTGG
    GGTGAACATT TTGAGTTCTA CAGCCTTCCA CCTCTTCATA GCATCACAGT TCACATTTAT
    AAAGATGTGG AAAAAAAGAA GAAAAAAGAC AAGAATAATT ATGTAGGGCT GGTCAACATC
    CCCACTGCCA GTGTGACTGG TCGCCAGTTT GTAGAAAAGT GGTACCCAGT GAGCACACCT
    ACACCCAACA AAGGAAAGAC AGGAGGACCT TCTATTCGGA TTAAGTCGCG TTTCCAAACC
    ATCACCATTC TGCCTATGGA GCAATACAAA GAGTTTGCAG AATTTGTCAC CAGCAACTAT
    ACCATGTTAT GTTCTGTTCT TGAACCAGTA ATCAGTGTGA GAAATAAAGA AGAGCTAGCC
    TGTGCCTTAG TGCACATTCT TCAAAGCACT GGAAGGGCCA AGGATTTTCT GACGGACTTG
    GTGATGTCTG AGGTGGATCG TTGTGGAGAA CATGATGTCT TGATCTTCAG AGAGAACACC
    ATTGCCACCA AATCCATTGA GGAATACCTC AAGTTGGTGG GACAACAGTA TCTTCACGAT
    GCACTGGGGG AGTTCATCAA AGCGTTGTAT GAGTCAGATG AAAACTGTGA AGTGGATCCC
    AGCAAATGTT CATCCAGTGA ACTTCTGGAC CATCAGAGCA ACCTGAAAAT GTGCTGTGAG
    CTGGCTTTCT GCAAGATCAT CAACTCTTAC TGTGTTTTTC CTCGTGAGCT GAAAGAAGTG
    TTTGCATCCT GGAAGCAGCA GTGCCTGAAC CGCGGCAAGC AGGACATCAG CGAGAGGCTC
    ATCAGCGCCT CGCTGTTTCT CCGTTTTCTG TGCCCGGCCA TCATGTCTCC CAGTCTCTTC
    AACCTCATGC AGGAGTATCC TGATGACCGC ACATCTCGGA CTCTGACTCT CATTGCCAAG
    GTCATCCAGA ACCTGGCCAA CTTTGCCAAA TTTGGTAACA AGGAGGAATA CATGGCATTC
    ATGAATGATT TTTTAGAACA TGAATGGGGT GGAATGAAGC GCTTTCTTTT GGAGATCTCT
    AATCCAGACA CCATCTCAAA CACCCCAGGC TTTGATGGTT ACATTGATCT GGGCCGAGAG
    CTTTCAGTTT TGCATTCCTT ACTATGGGAA GTAGTTTCCC AACTTGATAA GGGTGAAAAT
    TCCTTCCTAC AGGCAACCGT GGCAAAATTG GGACCTCTCC CTCGTGTTCT TGCTGATATT
    ACCAAATCTC TGACTAATCC TACACCAATA CAACAGCAAC TGAGACGCTT CACTGAGCAT
    AACTCCAGTC CAAATGTCAG TGGAAGCCTC TCCTCTGGGC TGCAGAAAAT ATTTGAAGAC
    CCTACTGACA GTGATTTGCA CAAACTAAAA TCCCCAAGCC AGGACAACAC AGACAGTTAT
    TTCAGAGGGA AGACATTATT GCTGGTCCAG CAAGCCTCCT CCCAGAGCAT GACTTACTCG
    GAAAAGGATG AAAAGGAAAG TAGCCTCCCC AATGGCCGCA GCATCTCCCT CATGGACCTT
    CAGGAGACGC ACGCAGCCCA AGCAGAGCAT CCATCTGTGA TACTCGACGT GCCCATGCGC
    TTGACCGGAA GCCAGCTCTC CATAACTCAG GTGGCCAGCA TCAAGCAACT GCGGGAGACC
    CAGAGCACCC CCCAGAGTGC ACCCCAAGTG CGAAGGCCCC TGCACCCAGC CTTGAACCAG
    CCAGGCAGCC TCCAGCCCCT GTCCTTCCAG AACCCTGTCT ATCACCTCAG TAACCCCATT
    CCTGCAATGC CAAAGGCCTC TGTGGACTCC AGTTTGGAGA ACCTAAGCAC TGCCAGTTCC
    AGGAGCCAAA GTAACAGTGA AGACTTCAAA CTCAGCGGAC CCAGCAACAG CAGCATGGAG
    GACTTCACCA AACGGAGCAC GCAGAGTGAG GACTTCTCCA GGCGCCACAC CATGCCCGAC
    AGACACATAC CTCTCGCCCT GCCGCGGCAG AACAGCACGG GGCAGACCCA GCTCCGGAAG
    ATGGACCCGG CGGGGCTGGG CGCCCGAGCC AAAGCCCCGC CGTCCCTGCC GCACAGCGCC
    TCCCTGCGGA GCACCGGGAG CATGTCGGTG GCATCAGCGG CTCTGGTGGC CGAGCCCCTG
    CAGAACGGGA GCCGGTCCCG ACAGCAGTCC TCTTCCTCCA GAGAGAGCCC GGTCCCCAAA
    GTGAGAGCCA TCCAGAGGCA GCAGACCCAG CAGGTTCAGT CACCTGTGGA CTCCGCCACA
    ATGTCCCCAG TCGAGAGGAC AGCAGCCTGG GTCCTGAACA ATGGGCAGTA TGAAGAGGAC
    GTGGAAGAGA CTGAGCAAAA TCAGGACGAA GCCAAGCATG CTGAGAAGTA CGAACAGGAA
    ATCACCAAAC TGAAGGAGCG CCTGCGCGTG TCCAGCCGGC GACTCGAGGA GTATGAGCGC
    CGGTTGCTAG TGCAGGAGCA GCAGATGCAG AAGCTGCTGC TGGAGTACAA GGCCCGGCTG
    GAGGACAGCG AGGAGCGGCT GCGCAGGCAG CAGGAGGAGA AGGACAGCCA GATGAAGAGC
    ATCATCAGCA GGCTAATGGC TGTGGAAGAG GAACTGAAGA AGGATCATGC TGAGATGCAG
    GCAGTTATTG ATGCAAAGCA AAAAATAATC GATGCACAGG AAAAACGGAT CGTGTCGCTG
    GATTCGGCCA ACACCAGACT GATGAGTGCG CTGACCCAGG TGAAGGAACG GTACAGCATG
    CAGGTCCGAA ATGGCATCTC CCCCACCAAC CCCACCAAGC TTTCCATCAC GGAGAATGGT
    GAATTCAAAA ACAGCAGCTG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa224040
    Clone ID Related Accession (Same CDS sequence) XM_003587091.5
    Accession Version XM_003587091.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3843bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ras GTPase-activating protein nGAP isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_003587091.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCTCT CCCTGTCGTC CGGAGGAGCC GCGGAGGCGC TGTCCTGGCC AGAGATGTTC 
    CCGGGATTGG AGTCCGACTC GCCGCTCCCC CCGGAGGAGC TGGACGCGGT TGTCCCGGTC
    AGTGGGGCCA TGGCCGGTGG CATGTTGGAT CGGATTCTTC TGGAGTCCGT GTGCCAGCAA
    CAGAGCTGGG TCCGCGTGTA CGATGTGAAA GGGCCACCTA CCCACCGTCT GTCTTGTGGC
    CAGTCGCCCT ACACCGACAC AACAACATGG GAGCGGAAGT ACTGCATCCT CACAGACAGC
    CAGCTGGTGT TGCTCAACAA GGAGAAGGAG ATACCTGTGG ACGGAGGACA AGAACAGCAG
    GCGGATTCCA CCAAAGGGCG ATGCCTGCGG AGAACTGTCA GTGTTCCTTC CGAGGGTCAG
    TTTCCTGAAT ACCCACCAGA GGGCGCCACT AAACTGGAGG TGCCAGCAGA AAGGTCCCCA
    CGCAGACGGA GTATCTCAGG GACCACTACA TCTGAGAAAC CCAACTCTAT GGACACTGCG
    AATACCTCAC CCTTCAAAGT ACCAGGGTTC TTCAGCAAGC GCCTAAAAGG CTCCATCAAG
    AGGACCAAAA GCCAGTCAAA GCTTGACCGA AACACGAGCT TTCGGCTTCC CTCTCTTCGC
    AATACCGATG ACAGGTCTCG TGGGCTGCCT AAACTAAAAG AGTCCCGTTC TCATGAATCT
    TTGCTGAGCC CATGCAGTGC CGTGGAATGT CTGGATCTCG GTAGAGGGGA GCCTGTGTCG
    GTAAAGCCAC TCCATAGCAG CATCCTTGGA CAAGACTTCT GCTTTGAGGT TACCTACTTA
    AGTGGAAGTA AATGCTTCAG TTGTAACTCT GCTTCAGAGA GAGATAAGTG GATGGAAAAC
    CTGCGCAGGA CAGTTCAACC AAATAAGGAC AATTGTAGAC GAGCTGAAAA TGTTCTCCGT
    TTATGGATTA TTGAAGCCAA GGACCTTGCC CCTAAAAAGA AATATTTCTG TGAACTGTGC
    CTTGATGATA CCCTCTTTGC TCGTACAACT AGCAAGACCA AAGCAGACAA TATTTTCTGG
    GGTGAACATT TTGAGTTCTA CAGCCTTCCA CCTCTTCATA GCATCACAGT TCACATTTAT
    AAAGATGTGG AAAAAAAGAA GAAAAAAGAC AAGAATAATT ATGTAGGGCT GGTCAACATC
    CCCACTGCCA GTGTGACTGG TCGCCAGTTT GTAGAAAAGT GGTACCCAGT GAGCACACCT
    ACACCCAACA AAGGAAAGAC AGGAGGACCT TCTATTCGGA TTAAGTCGCG TTTCCAAACC
    ATCACCATTC TGCCTATGGA GCAATACAAA GAGTTTGCAG AATTTGTCAC CAGCAACTAT
    ACCATGTTAT GTTCTGTTCT TGAACCAGTA ATCAGTGTGA GAAATAAAGA AGAGCTAGCC
    TGTGCCTTAG TGCACATTCT TCAAAGCACT GGAAGGGCCA AGGATTTTCT GACGGACTTG
    GTGATGTCTG AGGTGGATCG TTGTGGAGAA CATGATGTCT TGATCTTCAG AGAGAACACC
    ATTGCCACCA AATCCATTGA GGAATACCTC AAGTTGGTGG GACAACAGTA TCTTCACGAT
    GCACTGGGGG AGTTCATCAA AGCGTTGTAT GAGTCAGATG AAAACTGTGA AGTGGATCCC
    AGCAAATGTT CATCCAGTGA ACTTCTGGAC CATCAGAGCA ACCTGAAAAT GTGCTGTGAG
    CTGGCTTTCT GCAAGATCAT CAACTCTTAC TGTGTTTTTC CTCGTGAGCT GAAAGAAGTG
    TTTGCATCCT GGAAGCAGCA GTGCCTGAAC CGCGGCAAGC AGGACATCAG CGAGAGGCTC
    ATCAGCGCCT CGCTGTTTCT CCGTTTTCTG TGCCCGGCCA TCATGTCTCC CAGTCTCTTC
    AACCTCATGC AGGAGTATCC TGATGACCGC ACATCTCGGA CTCTGACTCT CATTGCCAAG
    GTCATCCAGA ACCTGGCCAA CTTTGCCAAA TTTGGTAACA AGGAGGAATA CATGGCATTC
    ATGAATGATT TTTTAGAACA TGAATGGGGT GGAATGAAGC GCTTTCTTTT GGAGATCTCT
    AATCCAGACA CCATCTCAAA CACCCCAGGC TTTGATGGTT ACATTGATCT GGGCCGAGAG
    CTTTCAGTTT TGCATTCCTT ACTATGGGAA GTAGTTTCCC AACTTGATAA GGCAACCGTG
    GCAAAATTGG GACCTCTCCC TCGTGTTCTT GCTGATATTA CCAAATCTCT GACTAATCCT
    ACACCAATAC AACAGCAACT GAGACGCTTC ACTGAGCATA ACTCCAGTCC AAATGTCAGT
    GGAAGCCTCT CCTCTGGGCT GCAGAAAATA TTTGAAGACC CTACTGACAG TGATTTGCAC
    AAACTAAAAT CCCCAAGCCA GGACAACACA GACAGTTATT TCAGAGGGAA GACATTATTG
    CTGGTCCAGC AAGCCTCCTC CCAGAGCATG ACTTACTCGG AAAAGGATGA AAAGGAAAGT
    AGCCTCCCCA ATGGCCGCAG CATCTCCCTC ATGGACCTTC AGGAGACGCA CGCAGCCCAA
    GCAGAGCATC CATCTGTGAT ACTCGACGTG CCCATGCGCT TGACCGGAAG CCAGCTCTCC
    ATAACTCAGG TGGCCAGCAT CAAGCAACTG CGGGAGACCC AGAGCACCCC CCAGAGTGCA
    CCCCAAGTGC GAAGGCCCCT GCACCCAGCC TTGAACCAGC CAGGCAGCCT CCAGCCCCTG
    TCCTTCCAGA ACCCTGTCTA TCACCTCAGT AACCCCATTC CTGCAATGCC AAAGGCCTCT
    GTGGACTCCA GTTTGGAGAA CCTAAGCACT GCCAGTTCCA GGAGCCAAAG TAACAGTGAA
    GACTTCAAAC TCAGCGGACC CAGCAACAGC AGCATGGAGG ACTTCACCAA ACGGAGCACG
    CAGAGTGAGG ACTTCTCCAG GCGCCACACC ATGCCCGACA GACACATACC TCTCGCCCTG
    CCGCGGCAGA ACAGCACGGG GCAGACCCAG CTCCGGAAGA TGGACCCGGC GGGGCTGGGC
    GCCCGAGCCA AAGCCCCGCC GTCCCTGCCG CACAGCGCCT CCCTGCGGAG CACCGGGAGC
    ATGTCGGTGG CATCAGCGGC TCTGGTGGCC GAGCCCCTGC AGAACGGGAG CCGGTCCCGA
    CAGCAGTCCT CTTCCTCCAG AGAGAGCCCG GTCCCCAAAG TGAGAGCCAT CCAGAGGCAG
    CAGACCCAGC AGGTTCAGTC ACCTGTGGAC TCCGCCACAA TGTCCCCAGT CGAGAGGACA
    GCAGCCTGGG TCCTGAACAA TGGGCAGTAT GAAGAGGACG TGGAAGAGAC TGAGCAAAAT
    CAGGACGAAG CCAAGCATGC TGAGAAGTAC GAACAGGAAA TCACCAAACT GAAGGAGCGC
    CTGCGCGTGT CCAGCCGGCG ACTCGAGGAG TATGAGCGCC GGTTGCTAGT GCAGGAGCAG
    CAGATGCAGA AGCTGCTGCT GGAGTACAAG GCCCGGCTGG AGGACAGCGA GGAGCGGCTG
    CGCAGGCAGC AGGAGGAGAA GGACAGCCAG ATGAAGAGCA TCATCAGCAG GCTAATGGCT
    GTGGAAGAGG AACTGAAGAA GGATCATGCT GAGATGCAGG CAGTTATTGA TGCAAAGCAA
    AAAATAATCG ATGCACAGGA AAAACGGATC GTGTCGCTGG ATTCGGCCAA CACCAGACTG
    ATGAGTGCGC TGACCCAGGT GAAGGAACGG TACAGCATGC AGGTCCGAAA TGGCATCTCC
    CCCACCAACC CCACCAAGCT TTCCATCACG GAGAATGGTG AATTCAAAAA CAGCAGCTGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003587139.2
    CDS696..4538
    Translation

    Target ORF information:

    RefSeq Version XM_003587091.5
    Organism Bos taurus(cattle)
    Definition Bos taurus RAS protein activator like 2 (RASAL2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003587091.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCTCT CCCTGTCGTC CGGAGGAGCC GCGGAGGCGC TGTCCTGGCC AGAGATGTTC 
    CCGGGATTGG AGTCCGACTC GCCGCTCCCC CCGGAGGAGC TGGACGCGGT TGTCCCGGTC
    AGTGGGGCCA TGGCCGGTGG CATGTTGGAT CGGATTCTTC TGGAGTCCGT GTGCCAGCAA
    CAGAGCTGGG TCCGCGTGTA CGATGTGAAA GGGCCACCTA CCCACCGTCT GTCTTGTGGC
    CAGTCGCCCT ACACCGACAC AACAACATGG GAGCGGAAGT ACTGCATCCT CACAGACAGC
    CAGCTGGTGT TGCTCAACAA GGAGAAGGAG ATACCTGTGG ACGGAGGACA AGAACAGCAG
    GCGGATTCCA CCAAAGGGCG ATGCCTGCGG AGAACTGTCA GTGTTCCTTC CGAGGGTCAG
    TTTCCTGAAT ACCCACCAGA GGGCGCCACT AAACTGGAGG TGCCAGCAGA AAGGTCCCCA
    CGCAGACGGA GTATCTCAGG GACCACTACA TCTGAGAAAC CCAACTCTAT GGACACTGCG
    AATACCTCAC CCTTCAAAGT ACCAGGGTTC TTCAGCAAGC GCCTAAAAGG CTCCATCAAG
    AGGACCAAAA GCCAGTCAAA GCTTGACCGA AACACGAGCT TTCGGCTTCC CTCTCTTCGC
    AATACCGATG ACAGGTCTCG TGGGCTGCCT AAACTAAAAG AGTCCCGTTC TCATGAATCT
    TTGCTGAGCC CATGCAGTGC CGTGGAATGT CTGGATCTCG GTAGAGGGGA GCCTGTGTCG
    GTAAAGCCAC TCCATAGCAG CATCCTTGGA CAAGACTTCT GCTTTGAGGT TACCTACTTA
    AGTGGAAGTA AATGCTTCAG TTGTAACTCT GCTTCAGAGA GAGATAAGTG GATGGAAAAC
    CTGCGCAGGA CAGTTCAACC AAATAAGGAC AATTGTAGAC GAGCTGAAAA TGTTCTCCGT
    TTATGGATTA TTGAAGCCAA GGACCTTGCC CCTAAAAAGA AATATTTCTG TGAACTGTGC
    CTTGATGATA CCCTCTTTGC TCGTACAACT AGCAAGACCA AAGCAGACAA TATTTTCTGG
    GGTGAACATT TTGAGTTCTA CAGCCTTCCA CCTCTTCATA GCATCACAGT TCACATTTAT
    AAAGATGTGG AAAAAAAGAA GAAAAAAGAC AAGAATAATT ATGTAGGGCT GGTCAACATC
    CCCACTGCCA GTGTGACTGG TCGCCAGTTT GTAGAAAAGT GGTACCCAGT GAGCACACCT
    ACACCCAACA AAGGAAAGAC AGGAGGACCT TCTATTCGGA TTAAGTCGCG TTTCCAAACC
    ATCACCATTC TGCCTATGGA GCAATACAAA GAGTTTGCAG AATTTGTCAC CAGCAACTAT
    ACCATGTTAT GTTCTGTTCT TGAACCAGTA ATCAGTGTGA GAAATAAAGA AGAGCTAGCC
    TGTGCCTTAG TGCACATTCT TCAAAGCACT GGAAGGGCCA AGGATTTTCT GACGGACTTG
    GTGATGTCTG AGGTGGATCG TTGTGGAGAA CATGATGTCT TGATCTTCAG AGAGAACACC
    ATTGCCACCA AATCCATTGA GGAATACCTC AAGTTGGTGG GACAACAGTA TCTTCACGAT
    GCACTGGGGG AGTTCATCAA AGCGTTGTAT GAGTCAGATG AAAACTGTGA AGTGGATCCC
    AGCAAATGTT CATCCAGTGA ACTTCTGGAC CATCAGAGCA ACCTGAAAAT GTGCTGTGAG
    CTGGCTTTCT GCAAGATCAT CAACTCTTAC TGTGTTTTTC CTCGTGAGCT GAAAGAAGTG
    TTTGCATCCT GGAAGCAGCA GTGCCTGAAC CGCGGCAAGC AGGACATCAG CGAGAGGCTC
    ATCAGCGCCT CGCTGTTTCT CCGTTTTCTG TGCCCGGCCA TCATGTCTCC CAGTCTCTTC
    AACCTCATGC AGGAGTATCC TGATGACCGC ACATCTCGGA CTCTGACTCT CATTGCCAAG
    GTCATCCAGA ACCTGGCCAA CTTTGCCAAA TTTGGTAACA AGGAGGAATA CATGGCATTC
    ATGAATGATT TTTTAGAACA TGAATGGGGT GGAATGAAGC GCTTTCTTTT GGAGATCTCT
    AATCCAGACA CCATCTCAAA CACCCCAGGC TTTGATGGTT ACATTGATCT GGGCCGAGAG
    CTTTCAGTTT TGCATTCCTT ACTATGGGAA GTAGTTTCCC AACTTGATAA GGCAACCGTG
    GCAAAATTGG GACCTCTCCC TCGTGTTCTT GCTGATATTA CCAAATCTCT GACTAATCCT
    ACACCAATAC AACAGCAACT GAGACGCTTC ACTGAGCATA ACTCCAGTCC AAATGTCAGT
    GGAAGCCTCT CCTCTGGGCT GCAGAAAATA TTTGAAGACC CTACTGACAG TGATTTGCAC
    AAACTAAAAT CCCCAAGCCA GGACAACACA GACAGTTATT TCAGAGGGAA GACATTATTG
    CTGGTCCAGC AAGCCTCCTC CCAGAGCATG ACTTACTCGG AAAAGGATGA AAAGGAAAGT
    AGCCTCCCCA ATGGCCGCAG CATCTCCCTC ATGGACCTTC AGGAGACGCA CGCAGCCCAA
    GCAGAGCATC CATCTGTGAT ACTCGACGTG CCCATGCGCT TGACCGGAAG CCAGCTCTCC
    ATAACTCAGG TGGCCAGCAT CAAGCAACTG CGGGAGACCC AGAGCACCCC CCAGAGTGCA
    CCCCAAGTGC GAAGGCCCCT GCACCCAGCC TTGAACCAGC CAGGCAGCCT CCAGCCCCTG
    TCCTTCCAGA ACCCTGTCTA TCACCTCAGT AACCCCATTC CTGCAATGCC AAAGGCCTCT
    GTGGACTCCA GTTTGGAGAA CCTAAGCACT GCCAGTTCCA GGAGCCAAAG TAACAGTGAA
    GACTTCAAAC TCAGCGGACC CAGCAACAGC AGCATGGAGG ACTTCACCAA ACGGAGCACG
    CAGAGTGAGG ACTTCTCCAG GCGCCACACC ATGCCCGACA GACACATACC TCTCGCCCTG
    CCGCGGCAGA ACAGCACGGG GCAGACCCAG CTCCGGAAGA TGGACCCGGC GGGGCTGGGC
    GCCCGAGCCA AAGCCCCGCC GTCCCTGCCG CACAGCGCCT CCCTGCGGAG CACCGGGAGC
    ATGTCGGTGG CATCAGCGGC TCTGGTGGCC GAGCCCCTGC AGAACGGGAG CCGGTCCCGA
    CAGCAGTCCT CTTCCTCCAG AGAGAGCCCG GTCCCCAAAG TGAGAGCCAT CCAGAGGCAG
    CAGACCCAGC AGGTTCAGTC ACCTGTGGAC TCCGCCACAA TGTCCCCAGT CGAGAGGACA
    GCAGCCTGGG TCCTGAACAA TGGGCAGTAT GAAGAGGACG TGGAAGAGAC TGAGCAAAAT
    CAGGACGAAG CCAAGCATGC TGAGAAGTAC GAACAGGAAA TCACCAAACT GAAGGAGCGC
    CTGCGCGTGT CCAGCCGGCG ACTCGAGGAG TATGAGCGCC GGTTGCTAGT GCAGGAGCAG
    CAGATGCAGA AGCTGCTGCT GGAGTACAAG GCCCGGCTGG AGGACAGCGA GGAGCGGCTG
    CGCAGGCAGC AGGAGGAGAA GGACAGCCAG ATGAAGAGCA TCATCAGCAG GCTAATGGCT
    GTGGAAGAGG AACTGAAGAA GGATCATGCT GAGATGCAGG CAGTTATTGA TGCAAAGCAA
    AAAATAATCG ATGCACAGGA AAAACGGATC GTGTCGCTGG ATTCGGCCAA CACCAGACTG
    ATGAGTGCGC TGACCCAGGT GAAGGAACGG TACAGCATGC AGGTCCGAAA TGGCATCTCC
    CCCACCAACC CCACCAAGCT TTCCATCACG GAGAATGGTG AATTCAAAAA CAGCAGCTGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa224041
    Clone ID Related Accession (Same CDS sequence) XM_024976868.1
    Accession Version XM_024976868.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3420bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ras GTPase-activating protein nGAP isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCAGACCC CAGAGGTGCC AGCAGAAAGG TCCCCACGCA GACGGAGTAT CTCAGGGACC 
    ACTACATCTG AGAAACCCAA CTCTATGGAC ACTGCGAATA CCTCACCCTT CAAAGTACCA
    GGGTTCTTCA GCAAGCGCCT AAAAGGCTCC ATCAAGAGGA CCAAAAGCCA GTCAAAGCTT
    GACCGAAACA CGAGCTTTCG GCTTCCCTCT CTTCGCAATA CCGATGACAG GTCTCGTGGG
    CTGCCTAAAC TAAAAGAGTC CCGTTCTCAT GAATCTTTGC TGAGCCCATG CAGTGCCGTG
    GAATGTCTGG ATCTCGGTAG AGGGGAGCCT GTGTCGGTAA AGCCACTCCA TAGCAGCATC
    CTTGGACAAG ACTTCTGCTT TGAGGTTACC TACTTAAGTG GAAGTAAATG CTTCAGTTGT
    AACTCTGCTT CAGAGAGAGA TAAGTGGATG GAAAACCTGC GCAGGACAGT TCAACCAAAT
    AAGGACAATT GTAGACGAGC TGAAAATGTT CTCCGTTTAT GGATTATTGA AGCCAAGGAC
    CTTGCCCCTA AAAAGAAATA TTTCTGTGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT
    ACAACTAGCA AGACCAAAGC AGACAATATT TTCTGGGGTG AACATTTTGA GTTCTACAGC
    CTTCCACCTC TTCATAGCAT CACAGTTCAC ATTTATAAAG ATGTGGAAAA AAAGAAGAAA
    AAAGACAAGA ATAATTATGT AGGGCTGGTC AACATCCCCA CTGCCAGTGT GACTGGTCGC
    CAGTTTGTAG AAAAGTGGTA CCCAGTGAGC ACACCTACAC CCAACAAAGG AAAGACAGGA
    GGACCTTCTA TTCGGATTAA GTCGCGTTTC CAAACCATCA CCATTCTGCC TATGGAGCAA
    TACAAAGAGT TTGCAGAATT TGTCACCAGC AACTATACCA TGTTATGTTC TGTTCTTGAA
    CCAGTAATCA GTGTGAGAAA TAAAGAAGAG CTAGCCTGTG CCTTAGTGCA CATTCTTCAA
    AGCACTGGAA GGGCCAAGGA TTTTCTGACG GACTTGGTGA TGTCTGAGGT GGATCGTTGT
    GGAGAACATG ATGTCTTGAT CTTCAGAGAG AACACCATTG CCACCAAATC CATTGAGGAA
    TACCTCAAGT TGGTGGGACA ACAGTATCTT CACGATGCAC TGGGGGAGTT CATCAAAGCG
    TTGTATGAGT CAGATGAAAA CTGTGAAGTG GATCCCAGCA AATGTTCATC CAGTGAACTT
    CTGGACCATC AGAGCAACCT GAAAATGTGC TGTGAGCTGG CTTTCTGCAA GATCATCAAC
    TCTTACTGTG TTTTTCCTCG TGAGCTGAAA GAAGTGTTTG CATCCTGGAA GCAGCAGTGC
    CTGAACCGCG GCAAGCAGGA CATCAGCGAG AGGCTCATCA GCGCCTCGCT GTTTCTCCGT
    TTTCTGTGCC CGGCCATCAT GTCTCCCAGT CTCTTCAACC TCATGCAGGA GTATCCTGAT
    GACCGCACAT CTCGGACTCT GACTCTCATT GCCAAGGTCA TCCAGAACCT GGCCAACTTT
    GCCAAATTTG GTAACAAGGA GGAATACATG GCATTCATGA ATGATTTTTT AGAACATGAA
    TGGGGTGGAA TGAAGCGCTT TCTTTTGGAG ATCTCTAATC CAGACACCAT CTCAAACACC
    CCAGGCTTTG ATGGTTACAT TGATCTGGGC CGAGAGCTTT CAGTTTTGCA TTCCTTACTA
    TGGGAAGTAG TTTCCCAACT TGATAAGGGT GAAAATTCCT TCCTACAGGC AACCGTGGCA
    AAATTGGGAC CTCTCCCTCG TGTTCTTGCT GATATTACCA AATCTCTGAC TAATCCTACA
    CCAATACAAC AGCAACTGAG ACGCTTCACT GAGCATAACT CCAGTCCAAA TGTCAGTGGA
    AGCCTCTCCT CTGGGCTGCA GAAAATATTT GAAGACCCTA CTGACAGTGA TTTGCACAAA
    CTAAAATCCC CAAGCCAGGA CAACACAGAC AGTTATTTCA GAGGGAAGAC ATTATTGCTG
    GTCCAGCAAG CCTCCTCCCA GAGCATGACT TACTCGGAAA AGGATGAAAA GGAAAGTAGC
    CTCCCCAATG GCCGCAGCAT CTCCCTCATG GACCTTCAGG AGACGCACGC AGCCCAAGCA
    GAGCATCCAT CTGTGATACT CGACGTGCCC ATGCGCTTGA CCGGAAGCCA GCTCTCCATA
    ACTCAGGTGG CCAGCATCAA GCAACTGCGG GAGACCCAGA GCACCCCCCA GAGTGCACCC
    CAAGTGCGAA GGCCCCTGCA CCCAGCCTTG AACCAGCCAG GCAGCCTCCA GCCCCTGTCC
    TTCCAGAACC CTGTCTATCA CCTCAGTAAC CCCATTCCTG CAATGCCAAA GGCCTCTGTG
    GACTCCAGTT TGGAGAACCT AAGCACTGCC AGTTCCAGGA GCCAAAGTAA CAGTGAAGAC
    TTCAAACTCA GCGGACCCAG CAACAGCAGC ATGGAGGACT TCACCAAACG GAGCACGCAG
    AGTGAGGACT TCTCCAGGCG CCACACCATG CCCGACAGAC ACATACCTCT CGCCCTGCCG
    CGGCAGAACA GCACGGGGCA GACCCAGCTC CGGAAGATGG ACCCGGCGGG GCTGGGCGCC
    CGAGCCAAAG CCCCGCCGTC CCTGCCGCAC AGCGCCTCCC TGCGGAGCAC CGGGAGCATG
    TCGGTGGCAT CAGCGGCTCT GGTGGCCGAG CCCCTGCAGA ACGGGAGCCG GTCCCGACAG
    CAGTCCTCTT CCTCCAGAGA GAGCCCGGTC CCCAAAGTGA GAGCCATCCA GAGGCAGCAG
    ACCCAGCAGG TTCAGTCACC TGTGGACTCC GCCACAATGT CCCCAGTCGA GAGGACAGCA
    GCCTGGGTCC TGAACAATGG GCAGTATGAA GAGGACGTGG AAGAGACTGA GCAAAATCAG
    GACGAAGCCA AGCATGCTGA GAAGTACGAA CAGGAAATCA CCAAACTGAA GGAGCGCCTG
    CGCGTGTCCA GCCGGCGACT CGAGGAGTAT GAGCGCCGGT TGCTAGTGCA GGAGCAGCAG
    ATGCAGAAGC TGCTGCTGGA GTACAAGGCC CGGCTGGAGG ACAGCGAGGA GCGGCTGCGC
    AGGCAGCAGG AGGAGAAGGA CAGCCAGATG AAGAGCATCA TCAGCAGGCT AATGGCTGTG
    GAAGAGGAAC TGAAGAAGGA TCATGCTGAG ATGCAGGCAG TTATTGATGC AAAGCAAAAA
    ATAATCGATG CACAGGAAAA ACGGATCGTG TCGCTGGATT CGGCCAACAC CAGACTGATG
    AGTGCGCTGA CCCAGGTGAA GGAACGGTAC AGCATGCAGG TCCGAAATGG CATCTCCCCC
    ACCAACCCCA CCAAGCTTTC CATCACGGAG AATGGTGAAT TCAAAAACAG CAGCTGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832636.1
    CDS142..3561
    Translation

    Target ORF information:

    RefSeq Version XM_024976868.1
    Organism Bos taurus(cattle)
    Definition Bos taurus RAS protein activator like 2 (RASAL2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976868.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCAGACCC CAGAGGTGCC AGCAGAAAGG TCCCCACGCA GACGGAGTAT CTCAGGGACC 
    ACTACATCTG AGAAACCCAA CTCTATGGAC ACTGCGAATA CCTCACCCTT CAAAGTACCA
    GGGTTCTTCA GCAAGCGCCT AAAAGGCTCC ATCAAGAGGA CCAAAAGCCA GTCAAAGCTT
    GACCGAAACA CGAGCTTTCG GCTTCCCTCT CTTCGCAATA CCGATGACAG GTCTCGTGGG
    CTGCCTAAAC TAAAAGAGTC CCGTTCTCAT GAATCTTTGC TGAGCCCATG CAGTGCCGTG
    GAATGTCTGG ATCTCGGTAG AGGGGAGCCT GTGTCGGTAA AGCCACTCCA TAGCAGCATC
    CTTGGACAAG ACTTCTGCTT TGAGGTTACC TACTTAAGTG GAAGTAAATG CTTCAGTTGT
    AACTCTGCTT CAGAGAGAGA TAAGTGGATG GAAAACCTGC GCAGGACAGT TCAACCAAAT
    AAGGACAATT GTAGACGAGC TGAAAATGTT CTCCGTTTAT GGATTATTGA AGCCAAGGAC
    CTTGCCCCTA AAAAGAAATA TTTCTGTGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT
    ACAACTAGCA AGACCAAAGC AGACAATATT TTCTGGGGTG AACATTTTGA GTTCTACAGC
    CTTCCACCTC TTCATAGCAT CACAGTTCAC ATTTATAAAG ATGTGGAAAA AAAGAAGAAA
    AAAGACAAGA ATAATTATGT AGGGCTGGTC AACATCCCCA CTGCCAGTGT GACTGGTCGC
    CAGTTTGTAG AAAAGTGGTA CCCAGTGAGC ACACCTACAC CCAACAAAGG AAAGACAGGA
    GGACCTTCTA TTCGGATTAA GTCGCGTTTC CAAACCATCA CCATTCTGCC TATGGAGCAA
    TACAAAGAGT TTGCAGAATT TGTCACCAGC AACTATACCA TGTTATGTTC TGTTCTTGAA
    CCAGTAATCA GTGTGAGAAA TAAAGAAGAG CTAGCCTGTG CCTTAGTGCA CATTCTTCAA
    AGCACTGGAA GGGCCAAGGA TTTTCTGACG GACTTGGTGA TGTCTGAGGT GGATCGTTGT
    GGAGAACATG ATGTCTTGAT CTTCAGAGAG AACACCATTG CCACCAAATC CATTGAGGAA
    TACCTCAAGT TGGTGGGACA ACAGTATCTT CACGATGCAC TGGGGGAGTT CATCAAAGCG
    TTGTATGAGT CAGATGAAAA CTGTGAAGTG GATCCCAGCA AATGTTCATC CAGTGAACTT
    CTGGACCATC AGAGCAACCT GAAAATGTGC TGTGAGCTGG CTTTCTGCAA GATCATCAAC
    TCTTACTGTG TTTTTCCTCG TGAGCTGAAA GAAGTGTTTG CATCCTGGAA GCAGCAGTGC
    CTGAACCGCG GCAAGCAGGA CATCAGCGAG AGGCTCATCA GCGCCTCGCT GTTTCTCCGT
    TTTCTGTGCC CGGCCATCAT GTCTCCCAGT CTCTTCAACC TCATGCAGGA GTATCCTGAT
    GACCGCACAT CTCGGACTCT GACTCTCATT GCCAAGGTCA TCCAGAACCT GGCCAACTTT
    GCCAAATTTG GTAACAAGGA GGAATACATG GCATTCATGA ATGATTTTTT AGAACATGAA
    TGGGGTGGAA TGAAGCGCTT TCTTTTGGAG ATCTCTAATC CAGACACCAT CTCAAACACC
    CCAGGCTTTG ATGGTTACAT TGATCTGGGC CGAGAGCTTT CAGTTTTGCA TTCCTTACTA
    TGGGAAGTAG TTTCCCAACT TGATAAGGGT GAAAATTCCT TCCTACAGGC AACCGTGGCA
    AAATTGGGAC CTCTCCCTCG TGTTCTTGCT GATATTACCA AATCTCTGAC TAATCCTACA
    CCAATACAAC AGCAACTGAG ACGCTTCACT GAGCATAACT CCAGTCCAAA TGTCAGTGGA
    AGCCTCTCCT CTGGGCTGCA GAAAATATTT GAAGACCCTA CTGACAGTGA TTTGCACAAA
    CTAAAATCCC CAAGCCAGGA CAACACAGAC AGTTATTTCA GAGGGAAGAC ATTATTGCTG
    GTCCAGCAAG CCTCCTCCCA GAGCATGACT TACTCGGAAA AGGATGAAAA GGAAAGTAGC
    CTCCCCAATG GCCGCAGCAT CTCCCTCATG GACCTTCAGG AGACGCACGC AGCCCAAGCA
    GAGCATCCAT CTGTGATACT CGACGTGCCC ATGCGCTTGA CCGGAAGCCA GCTCTCCATA
    ACTCAGGTGG CCAGCATCAA GCAACTGCGG GAGACCCAGA GCACCCCCCA GAGTGCACCC
    CAAGTGCGAA GGCCCCTGCA CCCAGCCTTG AACCAGCCAG GCAGCCTCCA GCCCCTGTCC
    TTCCAGAACC CTGTCTATCA CCTCAGTAAC CCCATTCCTG CAATGCCAAA GGCCTCTGTG
    GACTCCAGTT TGGAGAACCT AAGCACTGCC AGTTCCAGGA GCCAAAGTAA CAGTGAAGAC
    TTCAAACTCA GCGGACCCAG CAACAGCAGC ATGGAGGACT TCACCAAACG GAGCACGCAG
    AGTGAGGACT TCTCCAGGCG CCACACCATG CCCGACAGAC ACATACCTCT CGCCCTGCCG
    CGGCAGAACA GCACGGGGCA GACCCAGCTC CGGAAGATGG ACCCGGCGGG GCTGGGCGCC
    CGAGCCAAAG CCCCGCCGTC CCTGCCGCAC AGCGCCTCCC TGCGGAGCAC CGGGAGCATG
    TCGGTGGCAT CAGCGGCTCT GGTGGCCGAG CCCCTGCAGA ACGGGAGCCG GTCCCGACAG
    CAGTCCTCTT CCTCCAGAGA GAGCCCGGTC CCCAAAGTGA GAGCCATCCA GAGGCAGCAG
    ACCCAGCAGG TTCAGTCACC TGTGGACTCC GCCACAATGT CCCCAGTCGA GAGGACAGCA
    GCCTGGGTCC TGAACAATGG GCAGTATGAA GAGGACGTGG AAGAGACTGA GCAAAATCAG
    GACGAAGCCA AGCATGCTGA GAAGTACGAA CAGGAAATCA CCAAACTGAA GGAGCGCCTG
    CGCGTGTCCA GCCGGCGACT CGAGGAGTAT GAGCGCCGGT TGCTAGTGCA GGAGCAGCAG
    ATGCAGAAGC TGCTGCTGGA GTACAAGGCC CGGCTGGAGG ACAGCGAGGA GCGGCTGCGC
    AGGCAGCAGG AGGAGAAGGA CAGCCAGATG AAGAGCATCA TCAGCAGGCT AATGGCTGTG
    GAAGAGGAAC TGAAGAAGGA TCATGCTGAG ATGCAGGCAG TTATTGATGC AAAGCAAAAA
    ATAATCGATG CACAGGAAAA ACGGATCGTG TCGCTGGATT CGGCCAACAC CAGACTGATG
    AGTGCGCTGA CCCAGGTGAA GGAACGGTAC AGCATGCAGG TCCGAAATGG CATCTCCCCC
    ACCAACCCCA CCAAGCTTTC CATCACGGAG AATGGTGAAT TCAAAAACAG CAGCTGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa224042
    Clone ID Related Accession (Same CDS sequence) XM_024976869.1
    Accession Version XM_024976869.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3348bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ras GTPase-activating protein nGAP isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTTAGCAA CACCCAGAAA CTTCACGGAA TGCAGACCCC AGAGGTGCCA GCAGAAAGGT 
    CCCCACGCAG ACGGAGTATC TCAGGGACCA CTACATCTGA GAAACCCAAC TCTATGGACA
    CTGCGAATAC CTCACCCTTC AAAGTACCAG TCTCGTGGGC TGCCTAAACT AAAAGAGTCC
    CGTTCTCATG AATCTTTGCT GAGCCCATGC AGTGCCGTGG AATGTCTGGA TCTCGGTAGA
    GGGGAGCCTG TGTCGGTAAA GCCACTCCAT AGCAGCATCC TTGGACAAGA CTTCTGCTTT
    GAGGTTACCT ACTTAAGTGG AAGTAAATGC TTCAGTTGTA ACTCTGCTTC AGAGAGAGAT
    AAGTGGATGG AAAACCTGCG CAGGACAGTT CAACCAAATA AGGACAATTG TAGACGAGCT
    GAAAATGTTC TCCGTTTATG GATTATTGAA GCCAAGGACC TTGCCCCTAA AAAGAAATAT
    TTCTGTGAAC TGTGCCTTGA TGATACCCTC TTTGCTCGTA CAACTAGCAA GACCAAAGCA
    GACAATATTT TCTGGGGTGA ACATTTTGAG TTCTACAGCC TTCCACCTCT TCATAGCATC
    ACAGTTCACA TTTATAAAGA TGTGGAAAAA AAGAAGAAAA AAGACAAGAA TAATTATGTA
    GGGCTGGTCA ACATCCCCAC TGCCAGTGTG ACTGGTCGCC AGTTTGTAGA AAAGTGGTAC
    CCAGTGAGCA CACCTACACC CAACAAAGGA AAGACAGGAG GACCTTCTAT TCGGATTAAG
    TCGCGTTTCC AAACCATCAC CATTCTGCCT ATGGAGCAAT ACAAAGAGTT TGCAGAATTT
    GTCACCAGCA ACTATACCAT GTTATGTTCT GTTCTTGAAC CAGTAATCAG TGTGAGAAAT
    AAAGAAGAGC TAGCCTGTGC CTTAGTGCAC ATTCTTCAAA GCACTGGAAG GGCCAAGGAT
    TTTCTGACGG ACTTGGTGAT GTCTGAGGTG GATCGTTGTG GAGAACATGA TGTCTTGATC
    TTCAGAGAGA ACACCATTGC CACCAAATCC ATTGAGGAAT ACCTCAAGTT GGTGGGACAA
    CAGTATCTTC ACGATGCACT GGGGGAGTTC ATCAAAGCGT TGTATGAGTC AGATGAAAAC
    TGTGAAGTGG ATCCCAGCAA ATGTTCATCC AGTGAACTTC TGGACCATCA GAGCAACCTG
    AAAATGTGCT GTGAGCTGGC TTTCTGCAAG ATCATCAACT CTTACTGTGT TTTTCCTCGT
    GAGCTGAAAG AAGTGTTTGC ATCCTGGAAG CAGCAGTGCC TGAACCGCGG CAAGCAGGAC
    ATCAGCGAGA GGCTCATCAG CGCCTCGCTG TTTCTCCGTT TTCTGTGCCC GGCCATCATG
    TCTCCCAGTC TCTTCAACCT CATGCAGGAG TATCCTGATG ACCGCACATC TCGGACTCTG
    ACTCTCATTG CCAAGGTCAT CCAGAACCTG GCCAACTTTG CCAAATTTGG TAACAAGGAG
    GAATACATGG CATTCATGAA TGATTTTTTA GAACATGAAT GGGGTGGAAT GAAGCGCTTT
    CTTTTGGAGA TCTCTAATCC AGACACCATC TCAAACACCC CAGGCTTTGA TGGTTACATT
    GATCTGGGCC GAGAGCTTTC AGTTTTGCAT TCCTTACTAT GGGAAGTAGT TTCCCAACTT
    GATAAGGGTG AAAATTCCTT CCTACAGGCA ACCGTGGCAA AATTGGGACC TCTCCCTCGT
    GTTCTTGCTG ATATTACCAA ATCTCTGACT AATCCTACAC CAATACAACA GCAACTGAGA
    CGCTTCACTG AGCATAACTC CAGTCCAAAT GTCAGTGGAA GCCTCTCCTC TGGGCTGCAG
    AAAATATTTG AAGACCCTAC TGACAGTGAT TTGCACAAAC TAAAATCCCC AAGCCAGGAC
    AACACAGACA GTTATTTCAG AGGGAAGACA TTATTGCTGG TCCAGCAAGC CTCCTCCCAG
    AGCATGACTT ACTCGGAAAA GGATGAAAAG GAAAGTAGCC TCCCCAATGG CCGCAGCATC
    TCCCTCATGG ACCTTCAGGA GACGCACGCA GCCCAAGCAG AGCATCCATC TGTGATACTC
    GACGTGCCCA TGCGCTTGAC CGGAAGCCAG CTCTCCATAA CTCAGGTGGC CAGCATCAAG
    CAACTGCGGG AGACCCAGAG CACCCCCCAG AGTGCACCCC AAGTGCGAAG GCCCCTGCAC
    CCAGCCTTGA ACCAGCCAGG CAGCCTCCAG CCCCTGTCCT TCCAGAACCC TGTCTATCAC
    CTCAGTAACC CCATTCCTGC AATGCCAAAG GCCTCTGTGG ACTCCAGTTT GGAGAACCTA
    AGCACTGCCA GTTCCAGGAG CCAAAGTAAC AGTGAAGACT TCAAACTCAG CGGACCCAGC
    AACAGCAGCA TGGAGGACTT CACCAAACGG AGCACGCAGA GTGAGGACTT CTCCAGGCGC
    CACACCATGC CCGACAGACA CATACCTCTC GCCCTGCCGC GGCAGAACAG CACGGGGCAG
    ACCCAGCTCC GGAAGATGGA CCCGGCGGGG CTGGGCGCCC GAGCCAAAGC CCCGCCGTCC
    CTGCCGCACA GCGCCTCCCT GCGGAGCACC GGGAGCATGT CGGTGGCATC AGCGGCTCTG
    GTGGCCGAGC CCCTGCAGAA CGGGAGCCGG TCCCGACAGC AGTCCTCTTC CTCCAGAGAG
    AGCCCGGTCC CCAAAGTGAG AGCCATCCAG AGGCAGCAGA CCCAGCAGGT TCAGTCACCT
    GTGGACTCCG CCACAATGTC CCCAGTCGAG AGGACAGCAG CCTGGGTCCT GAACAATGGG
    CAGTATGAAG AGGACGTGGA AGAGACTGAG CAAAATCAGG ACGAAGCCAA GCATGCTGAG
    AAGTACGAAC AGGAAATCAC CAAACTGAAG GAGCGCCTGC GCGTGTCCAG CCGGCGACTC
    GAGGAGTATG AGCGCCGGTT GCTAGTGCAG GAGCAGCAGA TGCAGAAGCT GCTGCTGGAG
    TACAAGGCCC GGCTGGAGGA CAGCGAGGAG CGGCTGCGCA GGCAGCAGGA GGAGAAGGAC
    AGCCAGATGA AGAGCATCAT CAGCAGGCTA ATGGCTGTGG AAGAGGAACT GAAGAAGGAT
    CATGCTGAGA TGCAGGCAGT TATTGATGCA AAGCAAAAAA TAATCGATGC ACAGGTCATA
    AATGGAGATG AGATTATGCA AACAGGAAAA ACGGATCGTG TCGCTGGATT CGGCCAACAC
    CAGACTGATG AGTGCGCTGA CCCAGGTGAA GGAACGGTAC AGCATGCAGG TCCGAAATGG
    CATCTCCCCC ACCAACCCCA CCAAGCTTTC CATCACGGAG AATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832637.1
    CDS121..3468
    Translation

    Target ORF information:

    RefSeq Version XM_024976869.1
    Organism Bos taurus(cattle)
    Definition Bos taurus RAS protein activator like 2 (RASAL2), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976869.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTTAGCAA CACCCAGAAA CTTCACGGAA TGCAGACCCC AGAGGTGCCA GCAGAAAGGT 
    CCCCACGCAG ACGGAGTATC TCAGGGACCA CTACATCTGA GAAACCCAAC TCTATGGACA
    CTGCGAATAC CTCACCCTTC AAAGTACCAG TCTCGTGGGC TGCCTAAACT AAAAGAGTCC
    CGTTCTCATG AATCTTTGCT GAGCCCATGC AGTGCCGTGG AATGTCTGGA TCTCGGTAGA
    GGGGAGCCTG TGTCGGTAAA GCCACTCCAT AGCAGCATCC TTGGACAAGA CTTCTGCTTT
    GAGGTTACCT ACTTAAGTGG AAGTAAATGC TTCAGTTGTA ACTCTGCTTC AGAGAGAGAT
    AAGTGGATGG AAAACCTGCG CAGGACAGTT CAACCAAATA AGGACAATTG TAGACGAGCT
    GAAAATGTTC TCCGTTTATG GATTATTGAA GCCAAGGACC TTGCCCCTAA AAAGAAATAT
    TTCTGTGAAC TGTGCCTTGA TGATACCCTC TTTGCTCGTA CAACTAGCAA GACCAAAGCA
    GACAATATTT TCTGGGGTGA ACATTTTGAG TTCTACAGCC TTCCACCTCT TCATAGCATC
    ACAGTTCACA TTTATAAAGA TGTGGAAAAA AAGAAGAAAA AAGACAAGAA TAATTATGTA
    GGGCTGGTCA ACATCCCCAC TGCCAGTGTG ACTGGTCGCC AGTTTGTAGA AAAGTGGTAC
    CCAGTGAGCA CACCTACACC CAACAAAGGA AAGACAGGAG GACCTTCTAT TCGGATTAAG
    TCGCGTTTCC AAACCATCAC CATTCTGCCT ATGGAGCAAT ACAAAGAGTT TGCAGAATTT
    GTCACCAGCA ACTATACCAT GTTATGTTCT GTTCTTGAAC CAGTAATCAG TGTGAGAAAT
    AAAGAAGAGC TAGCCTGTGC CTTAGTGCAC ATTCTTCAAA GCACTGGAAG GGCCAAGGAT
    TTTCTGACGG ACTTGGTGAT GTCTGAGGTG GATCGTTGTG GAGAACATGA TGTCTTGATC
    TTCAGAGAGA ACACCATTGC CACCAAATCC ATTGAGGAAT ACCTCAAGTT GGTGGGACAA
    CAGTATCTTC ACGATGCACT GGGGGAGTTC ATCAAAGCGT TGTATGAGTC AGATGAAAAC
    TGTGAAGTGG ATCCCAGCAA ATGTTCATCC AGTGAACTTC TGGACCATCA GAGCAACCTG
    AAAATGTGCT GTGAGCTGGC TTTCTGCAAG ATCATCAACT CTTACTGTGT TTTTCCTCGT
    GAGCTGAAAG AAGTGTTTGC ATCCTGGAAG CAGCAGTGCC TGAACCGCGG CAAGCAGGAC
    ATCAGCGAGA GGCTCATCAG CGCCTCGCTG TTTCTCCGTT TTCTGTGCCC GGCCATCATG
    TCTCCCAGTC TCTTCAACCT CATGCAGGAG TATCCTGATG ACCGCACATC TCGGACTCTG
    ACTCTCATTG CCAAGGTCAT CCAGAACCTG GCCAACTTTG CCAAATTTGG TAACAAGGAG
    GAATACATGG CATTCATGAA TGATTTTTTA GAACATGAAT GGGGTGGAAT GAAGCGCTTT
    CTTTTGGAGA TCTCTAATCC AGACACCATC TCAAACACCC CAGGCTTTGA TGGTTACATT
    GATCTGGGCC GAGAGCTTTC AGTTTTGCAT TCCTTACTAT GGGAAGTAGT TTCCCAACTT
    GATAAGGGTG AAAATTCCTT CCTACAGGCA ACCGTGGCAA AATTGGGACC TCTCCCTCGT
    GTTCTTGCTG ATATTACCAA ATCTCTGACT AATCCTACAC CAATACAACA GCAACTGAGA
    CGCTTCACTG AGCATAACTC CAGTCCAAAT GTCAGTGGAA GCCTCTCCTC TGGGCTGCAG
    AAAATATTTG AAGACCCTAC TGACAGTGAT TTGCACAAAC TAAAATCCCC AAGCCAGGAC
    AACACAGACA GTTATTTCAG AGGGAAGACA TTATTGCTGG TCCAGCAAGC CTCCTCCCAG
    AGCATGACTT ACTCGGAAAA GGATGAAAAG GAAAGTAGCC TCCCCAATGG CCGCAGCATC
    TCCCTCATGG ACCTTCAGGA GACGCACGCA GCCCAAGCAG AGCATCCATC TGTGATACTC
    GACGTGCCCA TGCGCTTGAC CGGAAGCCAG CTCTCCATAA CTCAGGTGGC CAGCATCAAG
    CAACTGCGGG AGACCCAGAG CACCCCCCAG AGTGCACCCC AAGTGCGAAG GCCCCTGCAC
    CCAGCCTTGA ACCAGCCAGG CAGCCTCCAG CCCCTGTCCT TCCAGAACCC TGTCTATCAC
    CTCAGTAACC CCATTCCTGC AATGCCAAAG GCCTCTGTGG ACTCCAGTTT GGAGAACCTA
    AGCACTGCCA GTTCCAGGAG CCAAAGTAAC AGTGAAGACT TCAAACTCAG CGGACCCAGC
    AACAGCAGCA TGGAGGACTT CACCAAACGG AGCACGCAGA GTGAGGACTT CTCCAGGCGC
    CACACCATGC CCGACAGACA CATACCTCTC GCCCTGCCGC GGCAGAACAG CACGGGGCAG
    ACCCAGCTCC GGAAGATGGA CCCGGCGGGG CTGGGCGCCC GAGCCAAAGC CCCGCCGTCC
    CTGCCGCACA GCGCCTCCCT GCGGAGCACC GGGAGCATGT CGGTGGCATC AGCGGCTCTG
    GTGGCCGAGC CCCTGCAGAA CGGGAGCCGG TCCCGACAGC AGTCCTCTTC CTCCAGAGAG
    AGCCCGGTCC CCAAAGTGAG AGCCATCCAG AGGCAGCAGA CCCAGCAGGT TCAGTCACCT
    GTGGACTCCG CCACAATGTC CCCAGTCGAG AGGACAGCAG CCTGGGTCCT GAACAATGGG
    CAGTATGAAG AGGACGTGGA AGAGACTGAG CAAAATCAGG ACGAAGCCAA GCATGCTGAG
    AAGTACGAAC AGGAAATCAC CAAACTGAAG GAGCGCCTGC GCGTGTCCAG CCGGCGACTC
    GAGGAGTATG AGCGCCGGTT GCTAGTGCAG GAGCAGCAGA TGCAGAAGCT GCTGCTGGAG
    TACAAGGCCC GGCTGGAGGA CAGCGAGGAG CGGCTGCGCA GGCAGCAGGA GGAGAAGGAC
    AGCCAGATGA AGAGCATCAT CAGCAGGCTA ATGGCTGTGG AAGAGGAACT GAAGAAGGAT
    CATGCTGAGA TGCAGGCAGT TATTGATGCA AAGCAAAAAA TAATCGATGC ACAGGTCATA
    AATGGAGATG AGATTATGCA AACAGGAAAA ACGGATCGTG TCGCTGGATT CGGCCAACAC
    CAGACTGATG AGTGCGCTGA CCCAGGTGAA GGAACGGTAC AGCATGCAGG TCCGAAATGG
    CATCTCCCCC ACCAACCCCA CCAAGCTTTC CATCACGGAG AATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93019
    Clone ID Related Accession (Same CDS sequence) XM_015475217.1 , XM_015466925.1
    Accession Version XM_015475217.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3522bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ras GTPase-activating protein nGAP isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104439.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAAAAGT TTTCTGGCTG GAGAATGAGT CTCCTTTCCC CCGGTGATGT GGCCGAGTGG 
    TTGGATAGTA GCACGGAAGA GGAGAAGAGC CTTCTGTCAT TTCTGGAGGT GCCAGCAGAA
    AGGTCCCCAC GCAGACGGAG TATCTCAGGG ACCACTACAT CTGAGAAACC CAACTCTATG
    GACACTGCGA ATACCTCACC CTTCAAAGTA CCAGGGTTCT TCAGCAAGCG CCTAAAAGGC
    TCCATCAAGA GGACCAAAAG CCAGTCAAAG CTTGACCGAA ACACGAGCTT TCGGCTTCCC
    TCTCTTCGCA ATACCGATGA CAGGTCTCGT GGGCTGCCTA AACTAAAAGA GTCCCGTTCT
    CATGAATCTT TGCTGAGCCC ATGCAGTGCC GTGGAATGTC TGGATCTCGG TAGAGGGGAG
    CCTGTGTCGG TAAAGCCACT CCATAGCAGC ATCCTTGGAC AAGACTTCTG CTTTGAGGTT
    ACCTACTTAA GTGGAAGTAA ATGCTTCAGT TGTAACTCTG CTTCAGAGAG AGATAAGTGG
    ATGGAAAACC TGCGCAGGAC AGTTCAACCA AATAAGGACA ATTGTAGACG AGCTGAAAAT
    GTTCTCCGTT TATGGATTAT TGAAGCCAAG GACCTTGCCC CTAAAAAGAA ATATTTCTGT
    GAACTGTGCC TTGATGATAC CCTCTTTGCT CGTACAACTA GCAAGACCAA AGCAGACAAT
    ATTTTCTGGG GTGAACATTT TGAGTTCTAC AGCCTTCCAC CTCTTCATAG CATCACAGTT
    CACATTTATA AAGATGTGGA AAAAAAGAAG AAAAAAGACA AGAATAATTA TGTAGGGCTG
    GTCAACATCC CCACTGCCAG TGTGACTGGT CGCCAGTTTG TAGAAAAGTG GTACCCAGTG
    AGCACACCTA CACCCAACAA AGGAAAGACA GGAGGACCTT CTATTCGGAT TAAGTCGCGT
    TTCCAAACCA TCACCATTCT GCCTATGGAG CAATACAAAG AGTTTGCAGA ATTTGTCACC
    AGCAACTATA CCATGTTATG TTCTGTTCTT GAACCAGTAA TCAGTGTGAG AAATAAAGAA
    GAGCTAGCCT GTGCCTTAGT GCACATTCTT CAAAGCACTG GAAGGGCCAA GGATTTTCTG
    ACGGACTTGG TGATGTCTGA GGTGGATCGT TGTGGAGAAC ATGATGTCTT GATCTTCAGA
    GAGAACACCA TTGCCACCAA ATCCATTGAG GAATACCTCA AGTTGGTGGG ACAACAGTAT
    CTTCACGATG CACTGGGGGA GTTCATCAAA GCGTTGTATG AGTCAGATGA AAACTGTGAA
    GTGGATCCCA GCAAATGTTC ATCCAGTGAA CTTCTGGACC ATCAGAGCAA CCTGAAAATG
    TGCTGTGAGC TGGCTTTCTG CAAGATCATC AACTCTTACT GTGTTTTTCC TCGTGAGCTG
    AAAGAAGTGT TTGCATCCTG GAAGCAGCAG TGCCTGAACC GCGGCAAGCA GGACATCAGC
    GAGAGGCTCA TCAGCGCCTC GCTGTTTCTC CGTTTTCTGT GCCCGGCCAT CATGTCTCCC
    AGTCTCTTCA ACCTCATGCA GGAGTATCCT GATGACCGCA CATCTCGGAC TCTGACTCTC
    ATTGCCAAGG TCATCCAGAA CCTGGCCAAC TTTGCCAAAT TTGGTAACAA GGAGGAATAC
    ATGGCATTCA TGAATGATTT TTTAGAACAT GAATGGGGTG GAATGAAGCG CTTTCTTTTG
    GAGATCTCTA ATCCAGACAC CATCTCAAAC ACCCCAGGCT TTGATGGTTA CATTGATCTG
    GGCCGAGAGC TTTCAGTTTT GCATTCCTTA CTATGGGAAG TAGTTTCCCA ACTTGATAAG
    GGTGAAAATT CCTTCCTACA GGCAACCGTG GCAAAATTGG GACCTCTCCC TCGTGTTCTT
    GCTGATATTA CCAAATCTCT GACTAATCCT ACACCAATAC AACAGCAACT GAGACGCTTC
    ACTGAGCATA ACTCCAGTCC AAATGTCAGT GGAAGCCTCT CCTCTGGGCT GCAGAAAATA
    TTTGAAGACC CTACTGACAG TGATTTGCAC AAACTAAAAT CCCCAAGCCA GGACAACACA
    GACAGTTATT TCAGAGGGAA GACATTATTG CTGGTCCAGC AAGCCTCCTC CCAGAGCATG
    ACTTACTCGG AAAAGGATGA AAAGGAAAGT AGCCTCCCCA ATGGCCGCAG CATCTCCCTC
    ATGGACCTTC AGGAGACGCA CGCAGCCCAA GCAGAGCATC CATCTGTGAT ACTCGACGTG
    CCCATGCGCT TGACCGGAAG CCAGCTCTCC ATAACTCAGG TGGCCAGCAT CAAGCAACTG
    CGGGAGACCC AGAGCACCCC CCAGAGTGCA CCCCAAGTGC GAAGGCCCCT GCACCCAGCC
    TTGAACCAGC CAGGCAGCCT CCAGCCCCTG TCCTTCCAGA ACCCTGTCTA TCACCTCAGT
    AACCCCATTC CTGCAATGCC AAAGGCCTCT GTGGACTCCA GTTTGGAGAA CCTAAGCACT
    GCCAGTTCCA GGAGCCAAAG TAACAGTGAA GACTTCAAAC TCAGCGGACC CAGCAACAGC
    AGCATGGAGG ACTTCACCAA ACGGAGCACG CAGAGTGAGG ACTTCTCCAG GCGCCACACC
    ATGCCCGACA GACACATACC TCTCGCCCTG CCGCGGCAGA ACAGCACGGG GCAGACCCAG
    CTCCGGAAGA TGGACCCGGC GGGGCTGGGC GCCCGAGCCA AAGCCCCGCC GTCCCTGCCG
    CACAGCGCCT CCCTGCGGAG CACCGGGAGC ATGTCGGTGG CATCAGCGGC TCTGGTGGCC
    GAGCCCCTGC AGAACGGGAG CCGGTCCCGA CAGCAGTCCT CTTCCTCCAG AGAGAGCCCG
    GTCCCCAAAG TGAGAGCCAT CCAGAGGCAG CAGACCCAGC AGGTTCAGTC ACCTGTGGAC
    TCCGCCACAA TGTCCCCAGT CGAGAGGACA GCAGCCTGGG TCCTGAACAA TGGGCAGTAT
    GAAGAGGACG TGGAAGAGAC TGAGCAAAAT CAGGACGAAG CCAAGCATGC TGAGAAGTAC
    GAACAGGAAA TCACCAAACT GAAGGAGCGC CTGCGCGTGT CCAGCCGGCG ACTCGAGGAG
    TATGAGCGCC GGTTGCTAGT GCAGGAGCAG CAGATGCAGA AGCTGCTGCT GGAGTACAAG
    GCCCGGCTGG AGGACAGCGA GGAGCGGCTG CGCAGGCAGC AGGAGGAGAA GGACAGCCAG
    ATGAAGAGCA TCATCAGCAG GCTAATGGCT GTGGAAGAGG AACTGAAGAA GGATCATGCT
    GAGATGCAGG CAGTTATTGA TGCAAAGCAA AAAATAATCG ATGCACAGGT CATAAATGGA
    GATGAGATTA TGCAAACAGG AAAAACGGAT CGTGTCGCTG GATTCGGCCA ACACCAGACT
    GATGAGTGCG CTGACCCAGG TGAAGGAACG GTACAGCATG CAGGTCCGAA ATGGCATCTC
    CCCCACCAAC CCCACCAAGC TTTCCATCAC GGAGAATGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330703.1
    CDS1497..5018
    Translation

    Target ORF information:

    RefSeq Version XM_015475217.1
    Organism Bos taurus(cattle)
    Definition Bos taurus RAS protein activator like 2 (RASAL2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475217.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAAAAGT TTTCTGGCTG GAGAATGAGT CTCCTTTCCC CCGGTGATGT GGCCGAGTGG 
    TTGGATAGTA GCACGGAAGA GGAGAAGAGC CTTCTGTCAT TTCTGGAGGT GCCAGCAGAA
    AGGTCCCCAC GCAGACGGAG TATCTCAGGG ACCACTACAT CTGAGAAACC CAACTCTATG
    GACACTGCGA ATACCTCACC CTTCAAAGTA CCAGGGTTCT TCAGCAAGCG CCTAAAAGGC
    TCCATCAAGA GGACCAAAAG CCAGTCAAAG CTTGACCGAA ACACGAGCTT TCGGCTTCCC
    TCTCTTCGCA ATACCGATGA CAGGTCTCGT GGGCTGCCTA AACTAAAAGA GTCCCGTTCT
    CATGAATCTT TGCTGAGCCC ATGCAGTGCC GTGGAATGTC TGGATCTCGG TAGAGGGGAG
    CCTGTGTCGG TAAAGCCACT CCATAGCAGC ATCCTTGGAC AAGACTTCTG CTTTGAGGTT
    ACCTACTTAA GTGGAAGTAA ATGCTTCAGT TGTAACTCTG CTTCAGAGAG AGATAAGTGG
    ATGGAAAACC TGCGCAGGAC AGTTCAACCA AATAAGGACA ATTGTAGACG AGCTGAAAAT
    GTTCTCCGTT TATGGATTAT TGAAGCCAAG GACCTTGCCC CTAAAAAGAA ATATTTCTGT
    GAACTGTGCC TTGATGATAC CCTCTTTGCT CGTACAACTA GCAAGACCAA AGCAGACAAT
    ATTTTCTGGG GTGAACATTT TGAGTTCTAC AGCCTTCCAC CTCTTCATAG CATCACAGTT
    CACATTTATA AAGATGTGGA AAAAAAGAAG AAAAAAGACA AGAATAATTA TGTAGGGCTG
    GTCAACATCC CCACTGCCAG TGTGACTGGT CGCCAGTTTG TAGAAAAGTG GTACCCAGTG
    AGCACACCTA CACCCAACAA AGGAAAGACA GGAGGACCTT CTATTCGGAT TAAGTCGCGT
    TTCCAAACCA TCACCATTCT GCCTATGGAG CAATACAAAG AGTTTGCAGA ATTTGTCACC
    AGCAACTATA CCATGTTATG TTCTGTTCTT GAACCAGTAA TCAGTGTGAG AAATAAAGAA
    GAGCTAGCCT GTGCCTTAGT GCACATTCTT CAAAGCACTG GAAGGGCCAA GGATTTTCTG
    ACGGACTTGG TGATGTCTGA GGTGGATCGT TGTGGAGAAC ATGATGTCTT GATCTTCAGA
    GAGAACACCA TTGCCACCAA ATCCATTGAG GAATACCTCA AGTTGGTGGG ACAACAGTAT
    CTTCACGATG CACTGGGGGA GTTCATCAAA GCGTTGTATG AGTCAGATGA AAACTGTGAA
    GTGGATCCCA GCAAATGTTC ATCCAGTGAA CTTCTGGACC ATCAGAGCAA CCTGAAAATG
    TGCTGTGAGC TGGCTTTCTG CAAGATCATC AACTCTTACT GTGTTTTTCC TCGTGAGCTG
    AAAGAAGTGT TTGCATCCTG GAAGCAGCAG TGCCTGAACC GCGGCAAGCA GGACATCAGC
    GAGAGGCTCA TCAGCGCCTC GCTGTTTCTC CGTTTTCTGT GCCCGGCCAT CATGTCTCCC
    AGTCTCTTCA ACCTCATGCA GGAGTATCCT GATGACCGCA CATCTCGGAC TCTGACTCTC
    ATTGCCAAGG TCATCCAGAA CCTGGCCAAC TTTGCCAAAT TTGGTAACAA GGAGGAATAC
    ATGGCATTCA TGAATGATTT TTTAGAACAT GAATGGGGTG GAATGAAGCG CTTTCTTTTG
    GAGATCTCTA ATCCAGACAC CATCTCAAAC ACCCCAGGCT TTGATGGTTA CATTGATCTG
    GGCCGAGAGC TTTCAGTTTT GCATTCCTTA CTATGGGAAG TAGTTTCCCA ACTTGATAAG
    GGTGAAAATT CCTTCCTACA GGCAACCGTG GCAAAATTGG GACCTCTCCC TCGTGTTCTT
    GCTGATATTA CCAAATCTCT GACTAATCCT ACACCAATAC AACAGCAACT GAGACGCTTC
    ACTGAGCATA ACTCCAGTCC AAATGTCAGT GGAAGCCTCT CCTCTGGGCT GCAGAAAATA
    TTTGAAGACC CTACTGACAG TGATTTGCAC AAACTAAAAT CCCCAAGCCA GGACAACACA
    GACAGTTATT TCAGAGGGAA GACATTATTG CTGGTCCAGC AAGCCTCCTC CCAGAGCATG
    ACTTACTCGG AAAAGGATGA AAAGGAAAGT AGCCTCCCCA ATGGCCGCAG CATCTCCCTC
    ATGGACCTTC AGGAGACGCA CGCAGCCCAA GCAGAGCATC CATCTGTGAT ACTCGACGTG
    CCCATGCGCT TGACCGGAAG CCAGCTCTCC ATAACTCAGG TGGCCAGCAT CAAGCAACTG
    CGGGAGACCC AGAGCACCCC CCAGAGTGCA CCCCAAGTGC GAAGGCCCCT GCACCCAGCC
    TTGAACCAGC CAGGCAGCCT CCAGCCCCTG TCCTTCCAGA ACCCTGTCTA TCACCTCAGT
    AACCCCATTC CTGCAATGCC AAAGGCCTCT GTGGACTCCA GTTTGGAGAA CCTAAGCACT
    GCCAGTTCCA GGAGCCAAAG TAACAGTGAA GACTTCAAAC TCAGCGGACC CAGCAACAGC
    AGCATGGAGG ACTTCACCAA ACGGAGCACG CAGAGTGAGG ACTTCTCCAG GCGCCACACC
    ATGCCCGACA GACACATACC TCTCGCCCTG CCGCGGCAGA ACAGCACGGG GCAGACCCAG
    CTCCGGAAGA TGGACCCGGC GGGGCTGGGC GCCCGAGCCA AAGCCCCGCC GTCCCTGCCG
    CACAGCGCCT CCCTGCGGAG CACCGGGAGC ATGTCGGTGG CATCAGCGGC TCTGGTGGCC
    GAGCCCCTGC AGAACGGGAG CCGGTCCCGA CAGCAGTCCT CTTCCTCCAG AGAGAGCCCG
    GTCCCCAAAG TGAGAGCCAT CCAGAGGCAG CAGACCCAGC AGGTTCAGTC ACCTGTGGAC
    TCCGCCACAA TGTCCCCAGT CGAGAGGACA GCAGCCTGGG TCCTGAACAA TGGGCAGTAT
    GAAGAGGACG TGGAAGAGAC TGAGCAAAAT CAGGACGAAG CCAAGCATGC TGAGAAGTAC
    GAACAGGAAA TCACCAAACT GAAGGAGCGC CTGCGCGTGT CCAGCCGGCG ACTCGAGGAG
    TATGAGCGCC GGTTGCTAGT GCAGGAGCAG CAGATGCAGA AGCTGCTGCT GGAGTACAAG
    GCCCGGCTGG AGGACAGCGA GGAGCGGCTG CGCAGGCAGC AGGAGGAGAA GGACAGCCAG
    ATGAAGAGCA TCATCAGCAG GCTAATGGCT GTGGAAGAGG AACTGAAGAA GGATCATGCT
    GAGATGCAGG CAGTTATTGA TGCAAAGCAA AAAATAATCG ATGCACAGGT CATAAATGGA
    GATGAGATTA TGCAAACAGG AAAAACGGAT CGTGTCGCTG GATTCGGCCA ACACCAGACT
    GATGAGTGCG CTGACCCAGG TGAAGGAACG GTACAGCATG CAGGTCCGAA ATGGCATCTC
    CCCCACCAAC CCCACCAAGC TTTCCATCAC GGAGAATGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93019
    Clone ID Related Accession (Same CDS sequence) XM_015475217.1 , XM_015466925.1
    Accession Version XM_015466925.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3522bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ras GTPase-activating protein nGAP isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646324.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAAAAGT TTTCTGGCTG GAGAATGAGT CTCCTTTCCC CCGGTGATGT GGCCGAGTGG 
    TTGGATAGTA GCACGGAAGA GGAGAAGAGC CTTCTGTCAT TTCTGGAGGT GCCAGCAGAA
    AGGTCCCCAC GCAGACGGAG TATCTCAGGG ACCACTACAT CTGAGAAACC CAACTCTATG
    GACACTGCGA ATACCTCACC CTTCAAAGTA CCAGGGTTCT TCAGCAAGCG CCTAAAAGGC
    TCCATCAAGA GGACCAAAAG CCAGTCAAAG CTTGACCGAA ACACGAGCTT TCGGCTTCCC
    TCTCTTCGCA ATACCGATGA CAGGTCTCGT GGGCTGCCTA AACTAAAAGA GTCCCGTTCT
    CATGAATCTT TGCTGAGCCC ATGCAGTGCC GTGGAATGTC TGGATCTCGG TAGAGGGGAG
    CCTGTGTCGG TAAAGCCACT CCATAGCAGC ATCCTTGGAC AAGACTTCTG CTTTGAGGTT
    ACCTACTTAA GTGGAAGTAA ATGCTTCAGT TGTAACTCTG CTTCAGAGAG AGATAAGTGG
    ATGGAAAACC TGCGCAGGAC AGTTCAACCA AATAAGGACA ATTGTAGACG AGCTGAAAAT
    GTTCTCCGTT TATGGATTAT TGAAGCCAAG GACCTTGCCC CTAAAAAGAA ATATTTCTGT
    GAACTGTGCC TTGATGATAC CCTCTTTGCT CGTACAACTA GCAAGACCAA AGCAGACAAT
    ATTTTCTGGG GTGAACATTT TGAGTTCTAC AGCCTTCCAC CTCTTCATAG CATCACAGTT
    CACATTTATA AAGATGTGGA AAAAAAGAAG AAAAAAGACA AGAATAATTA TGTAGGGCTG
    GTCAACATCC CCACTGCCAG TGTGACTGGT CGCCAGTTTG TAGAAAAGTG GTACCCAGTG
    AGCACACCTA CACCCAACAA AGGAAAGACA GGAGGACCTT CTATTCGGAT TAAGTCGCGT
    TTCCAAACCA TCACCATTCT GCCTATGGAG CAATACAAAG AGTTTGCAGA ATTTGTCACC
    AGCAACTATA CCATGTTATG TTCTGTTCTT GAACCAGTAA TCAGTGTGAG AAATAAAGAA
    GAGCTAGCCT GTGCCTTAGT GCACATTCTT CAAAGCACTG GAAGGGCCAA GGATTTTCTG
    ACGGACTTGG TGATGTCTGA GGTGGATCGT TGTGGAGAAC ATGATGTCTT GATCTTCAGA
    GAGAACACCA TTGCCACCAA ATCCATTGAG GAATACCTCA AGTTGGTGGG ACAACAGTAT
    CTTCACGATG CACTGGGGGA GTTCATCAAA GCGTTGTATG AGTCAGATGA AAACTGTGAA
    GTGGATCCCA GCAAATGTTC ATCCAGTGAA CTTCTGGACC ATCAGAGCAA CCTGAAAATG
    TGCTGTGAGC TGGCTTTCTG CAAGATCATC AACTCTTACT GTGTTTTTCC TCGTGAGCTG
    AAAGAAGTGT TTGCATCCTG GAAGCAGCAG TGCCTGAACC GCGGCAAGCA GGACATCAGC
    GAGAGGCTCA TCAGCGCCTC GCTGTTTCTC CGTTTTCTGT GCCCGGCCAT CATGTCTCCC
    AGTCTCTTCA ACCTCATGCA GGAGTATCCT GATGACCGCA CATCTCGGAC TCTGACTCTC
    ATTGCCAAGG TCATCCAGAA CCTGGCCAAC TTTGCCAAAT TTGGTAACAA GGAGGAATAC
    ATGGCATTCA TGAATGATTT TTTAGAACAT GAATGGGGTG GAATGAAGCG CTTTCTTTTG
    GAGATCTCTA ATCCAGACAC CATCTCAAAC ACCCCAGGCT TTGATGGTTA CATTGATCTG
    GGCCGAGAGC TTTCAGTTTT GCATTCCTTA CTATGGGAAG TAGTTTCCCA ACTTGATAAG
    GGTGAAAATT CCTTCCTACA GGCAACCGTG GCAAAATTGG GACCTCTCCC TCGTGTTCTT
    GCTGATATTA CCAAATCTCT GACTAATCCT ACACCAATAC AACAGCAACT GAGACGCTTC
    ACTGAGCATA ACTCCAGTCC AAATGTCAGT GGAAGCCTCT CCTCTGGGCT GCAGAAAATA
    TTTGAAGACC CTACTGACAG TGATTTGCAC AAACTAAAAT CCCCAAGCCA GGACAACACA
    GACAGTTATT TCAGAGGGAA GACATTATTG CTGGTCCAGC AAGCCTCCTC CCAGAGCATG
    ACTTACTCGG AAAAGGATGA AAAGGAAAGT AGCCTCCCCA ATGGCCGCAG CATCTCCCTC
    ATGGACCTTC AGGAGACGCA CGCAGCCCAA GCAGAGCATC CATCTGTGAT ACTCGACGTG
    CCCATGCGCT TGACCGGAAG CCAGCTCTCC ATAACTCAGG TGGCCAGCAT CAAGCAACTG
    CGGGAGACCC AGAGCACCCC CCAGAGTGCA CCCCAAGTGC GAAGGCCCCT GCACCCAGCC
    TTGAACCAGC CAGGCAGCCT CCAGCCCCTG TCCTTCCAGA ACCCTGTCTA TCACCTCAGT
    AACCCCATTC CTGCAATGCC AAAGGCCTCT GTGGACTCCA GTTTGGAGAA CCTAAGCACT
    GCCAGTTCCA GGAGCCAAAG TAACAGTGAA GACTTCAAAC TCAGCGGACC CAGCAACAGC
    AGCATGGAGG ACTTCACCAA ACGGAGCACG CAGAGTGAGG ACTTCTCCAG GCGCCACACC
    ATGCCCGACA GACACATACC TCTCGCCCTG CCGCGGCAGA ACAGCACGGG GCAGACCCAG
    CTCCGGAAGA TGGACCCGGC GGGGCTGGGC GCCCGAGCCA AAGCCCCGCC GTCCCTGCCG
    CACAGCGCCT CCCTGCGGAG CACCGGGAGC ATGTCGGTGG CATCAGCGGC TCTGGTGGCC
    GAGCCCCTGC AGAACGGGAG CCGGTCCCGA CAGCAGTCCT CTTCCTCCAG AGAGAGCCCG
    GTCCCCAAAG TGAGAGCCAT CCAGAGGCAG CAGACCCAGC AGGTTCAGTC ACCTGTGGAC
    TCCGCCACAA TGTCCCCAGT CGAGAGGACA GCAGCCTGGG TCCTGAACAA TGGGCAGTAT
    GAAGAGGACG TGGAAGAGAC TGAGCAAAAT CAGGACGAAG CCAAGCATGC TGAGAAGTAC
    GAACAGGAAA TCACCAAACT GAAGGAGCGC CTGCGCGTGT CCAGCCGGCG ACTCGAGGAG
    TATGAGCGCC GGTTGCTAGT GCAGGAGCAG CAGATGCAGA AGCTGCTGCT GGAGTACAAG
    GCCCGGCTGG AGGACAGCGA GGAGCGGCTG CGCAGGCAGC AGGAGGAGAA GGACAGCCAG
    ATGAAGAGCA TCATCAGCAG GCTAATGGCT GTGGAAGAGG AACTGAAGAA GGATCATGCT
    GAGATGCAGG CAGTTATTGA TGCAAAGCAA AAAATAATCG ATGCACAGGT CATAAATGGA
    GATGAGATTA TGCAAACAGG AAAAACGGAT CGTGTCGCTG GATTCGGCCA ACACCAGACT
    GATGAGTGCG CTGACCCAGG TGAAGGAACG GTACAGCATG CAGGTCCGAA ATGGCATCTC
    CCCCACCAAC CCCACCAAGC TTTCCATCAC GGAGAATGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322411.1
    CDS1501..5022
    Translation

    Target ORF information:

    RefSeq Version XM_015466925.1
    Organism Bos taurus(cattle)
    Definition Bos taurus RAS protein activator like 2 (RASAL2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015466925.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAAAAGT TTTCTGGCTG GAGAATGAGT CTCCTTTCCC CCGGTGATGT GGCCGAGTGG 
    TTGGATAGTA GCACGGAAGA GGAGAAGAGC CTTCTGTCAT TTCTGGAGGT GCCAGCAGAA
    AGGTCCCCAC GCAGACGGAG TATCTCAGGG ACCACTACAT CTGAGAAACC CAACTCTATG
    GACACTGCGA ATACCTCACC CTTCAAAGTA CCAGGGTTCT TCAGCAAGCG CCTAAAAGGC
    TCCATCAAGA GGACCAAAAG CCAGTCAAAG CTTGACCGAA ACACGAGCTT TCGGCTTCCC
    TCTCTTCGCA ATACCGATGA CAGGTCTCGT GGGCTGCCTA AACTAAAAGA GTCCCGTTCT
    CATGAATCTT TGCTGAGCCC ATGCAGTGCC GTGGAATGTC TGGATCTCGG TAGAGGGGAG
    CCTGTGTCGG TAAAGCCACT CCATAGCAGC ATCCTTGGAC AAGACTTCTG CTTTGAGGTT
    ACCTACTTAA GTGGAAGTAA ATGCTTCAGT TGTAACTCTG CTTCAGAGAG AGATAAGTGG
    ATGGAAAACC TGCGCAGGAC AGTTCAACCA AATAAGGACA ATTGTAGACG AGCTGAAAAT
    GTTCTCCGTT TATGGATTAT TGAAGCCAAG GACCTTGCCC CTAAAAAGAA ATATTTCTGT
    GAACTGTGCC TTGATGATAC CCTCTTTGCT CGTACAACTA GCAAGACCAA AGCAGACAAT
    ATTTTCTGGG GTGAACATTT TGAGTTCTAC AGCCTTCCAC CTCTTCATAG CATCACAGTT
    CACATTTATA AAGATGTGGA AAAAAAGAAG AAAAAAGACA AGAATAATTA TGTAGGGCTG
    GTCAACATCC CCACTGCCAG TGTGACTGGT CGCCAGTTTG TAGAAAAGTG GTACCCAGTG
    AGCACACCTA CACCCAACAA AGGAAAGACA GGAGGACCTT CTATTCGGAT TAAGTCGCGT
    TTCCAAACCA TCACCATTCT GCCTATGGAG CAATACAAAG AGTTTGCAGA ATTTGTCACC
    AGCAACTATA CCATGTTATG TTCTGTTCTT GAACCAGTAA TCAGTGTGAG AAATAAAGAA
    GAGCTAGCCT GTGCCTTAGT GCACATTCTT CAAAGCACTG GAAGGGCCAA GGATTTTCTG
    ACGGACTTGG TGATGTCTGA GGTGGATCGT TGTGGAGAAC ATGATGTCTT GATCTTCAGA
    GAGAACACCA TTGCCACCAA ATCCATTGAG GAATACCTCA AGTTGGTGGG ACAACAGTAT
    CTTCACGATG CACTGGGGGA GTTCATCAAA GCGTTGTATG AGTCAGATGA AAACTGTGAA
    GTGGATCCCA GCAAATGTTC ATCCAGTGAA CTTCTGGACC ATCAGAGCAA CCTGAAAATG
    TGCTGTGAGC TGGCTTTCTG CAAGATCATC AACTCTTACT GTGTTTTTCC TCGTGAGCTG
    AAAGAAGTGT TTGCATCCTG GAAGCAGCAG TGCCTGAACC GCGGCAAGCA GGACATCAGC
    GAGAGGCTCA TCAGCGCCTC GCTGTTTCTC CGTTTTCTGT GCCCGGCCAT CATGTCTCCC
    AGTCTCTTCA ACCTCATGCA GGAGTATCCT GATGACCGCA CATCTCGGAC TCTGACTCTC
    ATTGCCAAGG TCATCCAGAA CCTGGCCAAC TTTGCCAAAT TTGGTAACAA GGAGGAATAC
    ATGGCATTCA TGAATGATTT TTTAGAACAT GAATGGGGTG GAATGAAGCG CTTTCTTTTG
    GAGATCTCTA ATCCAGACAC CATCTCAAAC ACCCCAGGCT TTGATGGTTA CATTGATCTG
    GGCCGAGAGC TTTCAGTTTT GCATTCCTTA CTATGGGAAG TAGTTTCCCA ACTTGATAAG
    GGTGAAAATT CCTTCCTACA GGCAACCGTG GCAAAATTGG GACCTCTCCC TCGTGTTCTT
    GCTGATATTA CCAAATCTCT GACTAATCCT ACACCAATAC AACAGCAACT GAGACGCTTC
    ACTGAGCATA ACTCCAGTCC AAATGTCAGT GGAAGCCTCT CCTCTGGGCT GCAGAAAATA
    TTTGAAGACC CTACTGACAG TGATTTGCAC AAACTAAAAT CCCCAAGCCA GGACAACACA
    GACAGTTATT TCAGAGGGAA GACATTATTG CTGGTCCAGC AAGCCTCCTC CCAGAGCATG
    ACTTACTCGG AAAAGGATGA AAAGGAAAGT AGCCTCCCCA ATGGCCGCAG CATCTCCCTC
    ATGGACCTTC AGGAGACGCA CGCAGCCCAA GCAGAGCATC CATCTGTGAT ACTCGACGTG
    CCCATGCGCT TGACCGGAAG CCAGCTCTCC ATAACTCAGG TGGCCAGCAT CAAGCAACTG
    CGGGAGACCC AGAGCACCCC CCAGAGTGCA CCCCAAGTGC GAAGGCCCCT GCACCCAGCC
    TTGAACCAGC CAGGCAGCCT CCAGCCCCTG TCCTTCCAGA ACCCTGTCTA TCACCTCAGT
    AACCCCATTC CTGCAATGCC AAAGGCCTCT GTGGACTCCA GTTTGGAGAA CCTAAGCACT
    GCCAGTTCCA GGAGCCAAAG TAACAGTGAA GACTTCAAAC TCAGCGGACC CAGCAACAGC
    AGCATGGAGG ACTTCACCAA ACGGAGCACG CAGAGTGAGG ACTTCTCCAG GCGCCACACC
    ATGCCCGACA GACACATACC TCTCGCCCTG CCGCGGCAGA ACAGCACGGG GCAGACCCAG
    CTCCGGAAGA TGGACCCGGC GGGGCTGGGC GCCCGAGCCA AAGCCCCGCC GTCCCTGCCG
    CACAGCGCCT CCCTGCGGAG CACCGGGAGC ATGTCGGTGG CATCAGCGGC TCTGGTGGCC
    GAGCCCCTGC AGAACGGGAG CCGGTCCCGA CAGCAGTCCT CTTCCTCCAG AGAGAGCCCG
    GTCCCCAAAG TGAGAGCCAT CCAGAGGCAG CAGACCCAGC AGGTTCAGTC ACCTGTGGAC
    TCCGCCACAA TGTCCCCAGT CGAGAGGACA GCAGCCTGGG TCCTGAACAA TGGGCAGTAT
    GAAGAGGACG TGGAAGAGAC TGAGCAAAAT CAGGACGAAG CCAAGCATGC TGAGAAGTAC
    GAACAGGAAA TCACCAAACT GAAGGAGCGC CTGCGCGTGT CCAGCCGGCG ACTCGAGGAG
    TATGAGCGCC GGTTGCTAGT GCAGGAGCAG CAGATGCAGA AGCTGCTGCT GGAGTACAAG
    GCCCGGCTGG AGGACAGCGA GGAGCGGCTG CGCAGGCAGC AGGAGGAGAA GGACAGCCAG
    ATGAAGAGCA TCATCAGCAG GCTAATGGCT GTGGAAGAGG AACTGAAGAA GGATCATGCT
    GAGATGCAGG CAGTTATTGA TGCAAAGCAA AAAATAATCG ATGCACAGGT CATAAATGGA
    GATGAGATTA TGCAAACAGG AAAAACGGAT CGTGTCGCTG GATTCGGCCA ACACCAGACT
    GATGAGTGCG CTGACCCAGG TGAAGGAACG GTACAGCATG CAGGTCCGAA ATGGCATCTC
    CCCCACCAAC CCCACCAAGC TTTCCATCAC GGAGAATGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93020
    Clone ID Related Accession (Same CDS sequence) XM_015466926.1 , XM_015475218.2 , XM_015475218.1
    Accession Version XM_015466926.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3438bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ras GTPase-activating protein nGAP isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646324.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGGAAGCA GTTGTGATAA AGAGGTGCCA GCAGAAAGGT CCCCACGCAG ACGGAGTATC 
    TCAGGGACCA CTACATCTGA GAAACCCAAC TCTATGGACA CTGCGAATAC CTCACCCTTC
    AAAGTACCAG GGTTCTTCAG CAAGCGCCTA AAAGGCTCCA TCAAGAGGAC CAAAAGCCAG
    TCAAAGCTTG ACCGAAACAC GAGCTTTCGG CTTCCCTCTC TTCGCAATAC CGATGACAGG
    TCTCGTGGGC TGCCTAAACT AAAAGAGTCC CGTTCTCATG AATCTTTGCT GAGCCCATGC
    AGTGCCGTGG AATGTCTGGA TCTCGGTAGA GGGGAGCCTG TGTCGGTAAA GCCACTCCAT
    AGCAGCATCC TTGGACAAGA CTTCTGCTTT GAGGTTACCT ACTTAAGTGG AAGTAAATGC
    TTCAGTTGTA ACTCTGCTTC AGAGAGAGAT AAGTGGATGG AAAACCTGCG CAGGACAGTT
    CAACCAAATA AGGACAATTG TAGACGAGCT GAAAATGTTC TCCGTTTATG GATTATTGAA
    GCCAAGGACC TTGCCCCTAA AAAGAAATAT TTCTGTGAAC TGTGCCTTGA TGATACCCTC
    TTTGCTCGTA CAACTAGCAA GACCAAAGCA GACAATATTT TCTGGGGTGA ACATTTTGAG
    TTCTACAGCC TTCCACCTCT TCATAGCATC ACAGTTCACA TTTATAAAGA TGTGGAAAAA
    AAGAAGAAAA AAGACAAGAA TAATTATGTA GGGCTGGTCA ACATCCCCAC TGCCAGTGTG
    ACTGGTCGCC AGTTTGTAGA AAAGTGGTAC CCAGTGAGCA CACCTACACC CAACAAAGGA
    AAGACAGGAG GACCTTCTAT TCGGATTAAG TCGCGTTTCC AAACCATCAC CATTCTGCCT
    ATGGAGCAAT ACAAAGAGTT TGCAGAATTT GTCACCAGCA ACTATACCAT GTTATGTTCT
    GTTCTTGAAC CAGTAATCAG TGTGAGAAAT AAAGAAGAGC TAGCCTGTGC CTTAGTGCAC
    ATTCTTCAAA GCACTGGAAG GGCCAAGGAT TTTCTGACGG ACTTGGTGAT GTCTGAGGTG
    GATCGTTGTG GAGAACATGA TGTCTTGATC TTCAGAGAGA ACACCATTGC CACCAAATCC
    ATTGAGGAAT ACCTCAAGTT GGTGGGACAA CAGTATCTTC ACGATGCACT GGGGGAGTTC
    ATCAAAGCGT TGTATGAGTC AGATGAAAAC TGTGAAGTGG ATCCCAGCAA ATGTTCATCC
    AGTGAACTTC TGGACCATCA GAGCAACCTG AAAATGTGCT GTGAGCTGGC TTTCTGCAAG
    ATCATCAACT CTTACTGTGT TTTTCCTCGT GAGCTGAAAG AAGTGTTTGC ATCCTGGAAG
    CAGCAGTGCC TGAACCGCGG CAAGCAGGAC ATCAGCGAGA GGCTCATCAG CGCCTCGCTG
    TTTCTCCGTT TTCTGTGCCC GGCCATCATG TCTCCCAGTC TCTTCAACCT CATGCAGGAG
    TATCCTGATG ACCGCACATC TCGGACTCTG ACTCTCATTG CCAAGGTCAT CCAGAACCTG
    GCCAACTTTG CCAAATTTGG TAACAAGGAG GAATACATGG CATTCATGAA TGATTTTTTA
    GAACATGAAT GGGGTGGAAT GAAGCGCTTT CTTTTGGAGA TCTCTAATCC AGACACCATC
    TCAAACACCC CAGGCTTTGA TGGTTACATT GATCTGGGCC GAGAGCTTTC AGTTTTGCAT
    TCCTTACTAT GGGAAGTAGT TTCCCAACTT GATAAGGGTG AAAATTCCTT CCTACAGGCA
    ACCGTGGCAA AATTGGGACC TCTCCCTCGT GTTCTTGCTG ATATTACCAA ATCTCTGACT
    AATCCTACAC CAATACAACA GCAACTGAGA CGCTTCACTG AGCATAACTC CAGTCCAAAT
    GTCAGTGGAA GCCTCTCCTC TGGGCTGCAG AAAATATTTG AAGACCCTAC TGACAGTGAT
    TTGCACAAAC TAAAATCCCC AAGCCAGGAC AACACAGACA GTTATTTCAG AGGGAAGACA
    TTATTGCTGG TCCAGCAAGC CTCCTCCCAG AGCATGACTT ACTCGGAAAA GGATGAAAAG
    GAAAGTAGCC TCCCCAATGG CCGCAGCATC TCCCTCATGG ACCTTCAGGA GACGCACGCA
    GCCCAAGCAG AGCATCCATC TGTGATACTC GACGTGCCCA TGCGCTTGAC CGGAAGCCAG
    CTCTCCATAA CTCAGGTGGC CAGCATCAAG CAACTGCGGG AGACCCAGAG CACCCCCCAG
    AGTGCACCCC AAGTGCGAAG GCCCCTGCAC CCAGCCTTGA ACCAGCCAGG CAGCCTCCAG
    CCCCTGTCCT TCCAGAACCC TGTCTATCAC CTCAGTAACC CCATTCCTGC AATGCCAAAG
    GCCTCTGTGG ACTCCAGTTT GGAGAACCTA AGCACTGCCA GTTCCAGGAG CCAAAGTAAC
    AGTGAAGACT TCAAACTCAG CGGACCCAGC AACAGCAGCA TGGAGGACTT CACCAAACGG
    AGCACGCAGA GTGAGGACTT CTCCAGGCGC CACACCATGC CCGACAGACA CATACCTCTC
    GCCCTGCCGC GGCAGAACAG CACGGGGCAG ACCCAGCTCC GGAAGATGGA CCCGGCGGGG
    CTGGGCGCCC GAGCCAAAGC CCCGCCGTCC CTGCCGCACA GCGCCTCCCT GCGGAGCACC
    GGGAGCATGT CGGTGGCATC AGCGGCTCTG GTGGCCGAGC CCCTGCAGAA CGGGAGCCGG
    TCCCGACAGC AGTCCTCTTC CTCCAGAGAG AGCCCGGTCC CCAAAGTGAG AGCCATCCAG
    AGGCAGCAGA CCCAGCAGGT TCAGTCACCT GTGGACTCCG CCACAATGTC CCCAGTCGAG
    AGGACAGCAG CCTGGGTCCT GAACAATGGG CAGTATGAAG AGGACGTGGA AGAGACTGAG
    CAAAATCAGG ACGAAGCCAA GCATGCTGAG AAGTACGAAC AGGAAATCAC CAAACTGAAG
    GAGCGCCTGC GCGTGTCCAG CCGGCGACTC GAGGAGTATG AGCGCCGGTT GCTAGTGCAG
    GAGCAGCAGA TGCAGAAGCT GCTGCTGGAG TACAAGGCCC GGCTGGAGGA CAGCGAGGAG
    CGGCTGCGCA GGCAGCAGGA GGAGAAGGAC AGCCAGATGA AGAGCATCAT CAGCAGGCTA
    ATGGCTGTGG AAGAGGAACT GAAGAAGGAT CATGCTGAGA TGCAGGCAGT TATTGATGCA
    AAGCAAAAAA TAATCGATGC ACAGGTCATA AATGGAGATG AGATTATGCA AACAGGAAAA
    ACGGATCGTG TCGCTGGATT CGGCCAACAC CAGACTGATG AGTGCGCTGA CCCAGGTGAA
    GGAACGGTAC AGCATGCAGG TCCGAAATGG CATCTCCCCC ACCAACCCCA CCAAGCTTTC
    CATCACGGAG AATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322412.1
    CDS70..3507
    Translation

    Target ORF information:

    RefSeq Version XM_015466926.1
    Organism Bos taurus(cattle)
    Definition Bos taurus RAS protein activator like 2 (RASAL2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015466926.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGGAAGCA GTTGTGATAA AGAGGTGCCA GCAGAAAGGT CCCCACGCAG ACGGAGTATC 
    TCAGGGACCA CTACATCTGA GAAACCCAAC TCTATGGACA CTGCGAATAC CTCACCCTTC
    AAAGTACCAG GGTTCTTCAG CAAGCGCCTA AAAGGCTCCA TCAAGAGGAC CAAAAGCCAG
    TCAAAGCTTG ACCGAAACAC GAGCTTTCGG CTTCCCTCTC TTCGCAATAC CGATGACAGG
    TCTCGTGGGC TGCCTAAACT AAAAGAGTCC CGTTCTCATG AATCTTTGCT GAGCCCATGC
    AGTGCCGTGG AATGTCTGGA TCTCGGTAGA GGGGAGCCTG TGTCGGTAAA GCCACTCCAT
    AGCAGCATCC TTGGACAAGA CTTCTGCTTT GAGGTTACCT ACTTAAGTGG AAGTAAATGC
    TTCAGTTGTA ACTCTGCTTC AGAGAGAGAT AAGTGGATGG AAAACCTGCG CAGGACAGTT
    CAACCAAATA AGGACAATTG TAGACGAGCT GAAAATGTTC TCCGTTTATG GATTATTGAA
    GCCAAGGACC TTGCCCCTAA AAAGAAATAT TTCTGTGAAC TGTGCCTTGA TGATACCCTC
    TTTGCTCGTA CAACTAGCAA GACCAAAGCA GACAATATTT TCTGGGGTGA ACATTTTGAG
    TTCTACAGCC TTCCACCTCT TCATAGCATC ACAGTTCACA TTTATAAAGA TGTGGAAAAA
    AAGAAGAAAA AAGACAAGAA TAATTATGTA GGGCTGGTCA ACATCCCCAC TGCCAGTGTG
    ACTGGTCGCC AGTTTGTAGA AAAGTGGTAC CCAGTGAGCA CACCTACACC CAACAAAGGA
    AAGACAGGAG GACCTTCTAT TCGGATTAAG TCGCGTTTCC AAACCATCAC CATTCTGCCT
    ATGGAGCAAT ACAAAGAGTT TGCAGAATTT GTCACCAGCA ACTATACCAT GTTATGTTCT
    GTTCTTGAAC CAGTAATCAG TGTGAGAAAT AAAGAAGAGC TAGCCTGTGC CTTAGTGCAC
    ATTCTTCAAA GCACTGGAAG GGCCAAGGAT TTTCTGACGG ACTTGGTGAT GTCTGAGGTG
    GATCGTTGTG GAGAACATGA TGTCTTGATC TTCAGAGAGA ACACCATTGC CACCAAATCC
    ATTGAGGAAT ACCTCAAGTT GGTGGGACAA CAGTATCTTC ACGATGCACT GGGGGAGTTC
    ATCAAAGCGT TGTATGAGTC AGATGAAAAC TGTGAAGTGG ATCCCAGCAA ATGTTCATCC
    AGTGAACTTC TGGACCATCA GAGCAACCTG AAAATGTGCT GTGAGCTGGC TTTCTGCAAG
    ATCATCAACT CTTACTGTGT TTTTCCTCGT GAGCTGAAAG AAGTGTTTGC ATCCTGGAAG
    CAGCAGTGCC TGAACCGCGG CAAGCAGGAC ATCAGCGAGA GGCTCATCAG CGCCTCGCTG
    TTTCTCCGTT TTCTGTGCCC GGCCATCATG TCTCCCAGTC TCTTCAACCT CATGCAGGAG
    TATCCTGATG ACCGCACATC TCGGACTCTG ACTCTCATTG CCAAGGTCAT CCAGAACCTG
    GCCAACTTTG CCAAATTTGG TAACAAGGAG GAATACATGG CATTCATGAA TGATTTTTTA
    GAACATGAAT GGGGTGGAAT GAAGCGCTTT CTTTTGGAGA TCTCTAATCC AGACACCATC
    TCAAACACCC CAGGCTTTGA TGGTTACATT GATCTGGGCC GAGAGCTTTC AGTTTTGCAT
    TCCTTACTAT GGGAAGTAGT TTCCCAACTT GATAAGGGTG AAAATTCCTT CCTACAGGCA
    ACCGTGGCAA AATTGGGACC TCTCCCTCGT GTTCTTGCTG ATATTACCAA ATCTCTGACT
    AATCCTACAC CAATACAACA GCAACTGAGA CGCTTCACTG AGCATAACTC CAGTCCAAAT
    GTCAGTGGAA GCCTCTCCTC TGGGCTGCAG AAAATATTTG AAGACCCTAC TGACAGTGAT
    TTGCACAAAC TAAAATCCCC AAGCCAGGAC AACACAGACA GTTATTTCAG AGGGAAGACA
    TTATTGCTGG TCCAGCAAGC CTCCTCCCAG AGCATGACTT ACTCGGAAAA GGATGAAAAG
    GAAAGTAGCC TCCCCAATGG CCGCAGCATC TCCCTCATGG ACCTTCAGGA GACGCACGCA
    GCCCAAGCAG AGCATCCATC TGTGATACTC GACGTGCCCA TGCGCTTGAC CGGAAGCCAG
    CTCTCCATAA CTCAGGTGGC CAGCATCAAG CAACTGCGGG AGACCCAGAG CACCCCCCAG
    AGTGCACCCC AAGTGCGAAG GCCCCTGCAC CCAGCCTTGA ACCAGCCAGG CAGCCTCCAG
    CCCCTGTCCT TCCAGAACCC TGTCTATCAC CTCAGTAACC CCATTCCTGC AATGCCAAAG
    GCCTCTGTGG ACTCCAGTTT GGAGAACCTA AGCACTGCCA GTTCCAGGAG CCAAAGTAAC
    AGTGAAGACT TCAAACTCAG CGGACCCAGC AACAGCAGCA TGGAGGACTT CACCAAACGG
    AGCACGCAGA GTGAGGACTT CTCCAGGCGC CACACCATGC CCGACAGACA CATACCTCTC
    GCCCTGCCGC GGCAGAACAG CACGGGGCAG ACCCAGCTCC GGAAGATGGA CCCGGCGGGG
    CTGGGCGCCC GAGCCAAAGC CCCGCCGTCC CTGCCGCACA GCGCCTCCCT GCGGAGCACC
    GGGAGCATGT CGGTGGCATC AGCGGCTCTG GTGGCCGAGC CCCTGCAGAA CGGGAGCCGG
    TCCCGACAGC AGTCCTCTTC CTCCAGAGAG AGCCCGGTCC CCAAAGTGAG AGCCATCCAG
    AGGCAGCAGA CCCAGCAGGT TCAGTCACCT GTGGACTCCG CCACAATGTC CCCAGTCGAG
    AGGACAGCAG CCTGGGTCCT GAACAATGGG CAGTATGAAG AGGACGTGGA AGAGACTGAG
    CAAAATCAGG ACGAAGCCAA GCATGCTGAG AAGTACGAAC AGGAAATCAC CAAACTGAAG
    GAGCGCCTGC GCGTGTCCAG CCGGCGACTC GAGGAGTATG AGCGCCGGTT GCTAGTGCAG
    GAGCAGCAGA TGCAGAAGCT GCTGCTGGAG TACAAGGCCC GGCTGGAGGA CAGCGAGGAG
    CGGCTGCGCA GGCAGCAGGA GGAGAAGGAC AGCCAGATGA AGAGCATCAT CAGCAGGCTA
    ATGGCTGTGG AAGAGGAACT GAAGAAGGAT CATGCTGAGA TGCAGGCAGT TATTGATGCA
    AAGCAAAAAA TAATCGATGC ACAGGTCATA AATGGAGATG AGATTATGCA AACAGGAAAA
    ACGGATCGTG TCGCTGGATT CGGCCAACAC CAGACTGATG AGTGCGCTGA CCCAGGTGAA
    GGAACGGTAC AGCATGCAGG TCCGAAATGG CATCTCCCCC ACCAACCCCA CCAAGCTTTC
    CATCACGGAG AATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93020
    Clone ID Related Accession (Same CDS sequence) XM_015466926.1 , XM_015475218.2 , XM_015475218.1
    Accession Version XM_015475218.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3438bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ras GTPase-activating protein nGAP isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015475218.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGGAAGCA GTTGTGATAA AGAGGTGCCA GCAGAAAGGT CCCCACGCAG ACGGAGTATC 
    TCAGGGACCA CTACATCTGA GAAACCCAAC TCTATGGACA CTGCGAATAC CTCACCCTTC
    AAAGTACCAG GGTTCTTCAG CAAGCGCCTA AAAGGCTCCA TCAAGAGGAC CAAAAGCCAG
    TCAAAGCTTG ACCGAAACAC GAGCTTTCGG CTTCCCTCTC TTCGCAATAC CGATGACAGG
    TCTCGTGGGC TGCCTAAACT AAAAGAGTCC CGTTCTCATG AATCTTTGCT GAGCCCATGC
    AGTGCCGTGG AATGTCTGGA TCTCGGTAGA GGGGAGCCTG TGTCGGTAAA GCCACTCCAT
    AGCAGCATCC TTGGACAAGA CTTCTGCTTT GAGGTTACCT ACTTAAGTGG AAGTAAATGC
    TTCAGTTGTA ACTCTGCTTC AGAGAGAGAT AAGTGGATGG AAAACCTGCG CAGGACAGTT
    CAACCAAATA AGGACAATTG TAGACGAGCT GAAAATGTTC TCCGTTTATG GATTATTGAA
    GCCAAGGACC TTGCCCCTAA AAAGAAATAT TTCTGTGAAC TGTGCCTTGA TGATACCCTC
    TTTGCTCGTA CAACTAGCAA GACCAAAGCA GACAATATTT TCTGGGGTGA ACATTTTGAG
    TTCTACAGCC TTCCACCTCT TCATAGCATC ACAGTTCACA TTTATAAAGA TGTGGAAAAA
    AAGAAGAAAA AAGACAAGAA TAATTATGTA GGGCTGGTCA ACATCCCCAC TGCCAGTGTG
    ACTGGTCGCC AGTTTGTAGA AAAGTGGTAC CCAGTGAGCA CACCTACACC CAACAAAGGA
    AAGACAGGAG GACCTTCTAT TCGGATTAAG TCGCGTTTCC AAACCATCAC CATTCTGCCT
    ATGGAGCAAT ACAAAGAGTT TGCAGAATTT GTCACCAGCA ACTATACCAT GTTATGTTCT
    GTTCTTGAAC CAGTAATCAG TGTGAGAAAT AAAGAAGAGC TAGCCTGTGC CTTAGTGCAC
    ATTCTTCAAA GCACTGGAAG GGCCAAGGAT TTTCTGACGG ACTTGGTGAT GTCTGAGGTG
    GATCGTTGTG GAGAACATGA TGTCTTGATC TTCAGAGAGA ACACCATTGC CACCAAATCC
    ATTGAGGAAT ACCTCAAGTT GGTGGGACAA CAGTATCTTC ACGATGCACT GGGGGAGTTC
    ATCAAAGCGT TGTATGAGTC AGATGAAAAC TGTGAAGTGG ATCCCAGCAA ATGTTCATCC
    AGTGAACTTC TGGACCATCA GAGCAACCTG AAAATGTGCT GTGAGCTGGC TTTCTGCAAG
    ATCATCAACT CTTACTGTGT TTTTCCTCGT GAGCTGAAAG AAGTGTTTGC ATCCTGGAAG
    CAGCAGTGCC TGAACCGCGG CAAGCAGGAC ATCAGCGAGA GGCTCATCAG CGCCTCGCTG
    TTTCTCCGTT TTCTGTGCCC GGCCATCATG TCTCCCAGTC TCTTCAACCT CATGCAGGAG
    TATCCTGATG ACCGCACATC TCGGACTCTG ACTCTCATTG CCAAGGTCAT CCAGAACCTG
    GCCAACTTTG CCAAATTTGG TAACAAGGAG GAATACATGG CATTCATGAA TGATTTTTTA
    GAACATGAAT GGGGTGGAAT GAAGCGCTTT CTTTTGGAGA TCTCTAATCC AGACACCATC
    TCAAACACCC CAGGCTTTGA TGGTTACATT GATCTGGGCC GAGAGCTTTC AGTTTTGCAT
    TCCTTACTAT GGGAAGTAGT TTCCCAACTT GATAAGGGTG AAAATTCCTT CCTACAGGCA
    ACCGTGGCAA AATTGGGACC TCTCCCTCGT GTTCTTGCTG ATATTACCAA ATCTCTGACT
    AATCCTACAC CAATACAACA GCAACTGAGA CGCTTCACTG AGCATAACTC CAGTCCAAAT
    GTCAGTGGAA GCCTCTCCTC TGGGCTGCAG AAAATATTTG AAGACCCTAC TGACAGTGAT
    TTGCACAAAC TAAAATCCCC AAGCCAGGAC AACACAGACA GTTATTTCAG AGGGAAGACA
    TTATTGCTGG TCCAGCAAGC CTCCTCCCAG AGCATGACTT ACTCGGAAAA GGATGAAAAG
    GAAAGTAGCC TCCCCAATGG CCGCAGCATC TCCCTCATGG ACCTTCAGGA GACGCACGCA
    GCCCAAGCAG AGCATCCATC TGTGATACTC GACGTGCCCA TGCGCTTGAC CGGAAGCCAG
    CTCTCCATAA CTCAGGTGGC CAGCATCAAG CAACTGCGGG AGACCCAGAG CACCCCCCAG
    AGTGCACCCC AAGTGCGAAG GCCCCTGCAC CCAGCCTTGA ACCAGCCAGG CAGCCTCCAG
    CCCCTGTCCT TCCAGAACCC TGTCTATCAC CTCAGTAACC CCATTCCTGC AATGCCAAAG
    GCCTCTGTGG ACTCCAGTTT GGAGAACCTA AGCACTGCCA GTTCCAGGAG CCAAAGTAAC
    AGTGAAGACT TCAAACTCAG CGGACCCAGC AACAGCAGCA TGGAGGACTT CACCAAACGG
    AGCACGCAGA GTGAGGACTT CTCCAGGCGC CACACCATGC CCGACAGACA CATACCTCTC
    GCCCTGCCGC GGCAGAACAG CACGGGGCAG ACCCAGCTCC GGAAGATGGA CCCGGCGGGG
    CTGGGCGCCC GAGCCAAAGC CCCGCCGTCC CTGCCGCACA GCGCCTCCCT GCGGAGCACC
    GGGAGCATGT CGGTGGCATC AGCGGCTCTG GTGGCCGAGC CCCTGCAGAA CGGGAGCCGG
    TCCCGACAGC AGTCCTCTTC CTCCAGAGAG AGCCCGGTCC CCAAAGTGAG AGCCATCCAG
    AGGCAGCAGA CCCAGCAGGT TCAGTCACCT GTGGACTCCG CCACAATGTC CCCAGTCGAG
    AGGACAGCAG CCTGGGTCCT GAACAATGGG CAGTATGAAG AGGACGTGGA AGAGACTGAG
    CAAAATCAGG ACGAAGCCAA GCATGCTGAG AAGTACGAAC AGGAAATCAC CAAACTGAAG
    GAGCGCCTGC GCGTGTCCAG CCGGCGACTC GAGGAGTATG AGCGCCGGTT GCTAGTGCAG
    GAGCAGCAGA TGCAGAAGCT GCTGCTGGAG TACAAGGCCC GGCTGGAGGA CAGCGAGGAG
    CGGCTGCGCA GGCAGCAGGA GGAGAAGGAC AGCCAGATGA AGAGCATCAT CAGCAGGCTA
    ATGGCTGTGG AAGAGGAACT GAAGAAGGAT CATGCTGAGA TGCAGGCAGT TATTGATGCA
    AAGCAAAAAA TAATCGATGC ACAGGTCATA AATGGAGATG AGATTATGCA AACAGGAAAA
    ACGGATCGTG TCGCTGGATT CGGCCAACAC CAGACTGATG AGTGCGCTGA CCCAGGTGAA
    GGAACGGTAC AGCATGCAGG TCCGAAATGG CATCTCCCCC ACCAACCCCA CCAAGCTTTC
    CATCACGGAG AATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330704.1
    CDS388..3825
    Translation

    Target ORF information:

    RefSeq Version XM_015475218.2
    Organism Bos taurus(cattle)
    Definition Bos taurus RAS protein activator like 2 (RASAL2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475218.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGGAAGCA GTTGTGATAA AGAGGTGCCA GCAGAAAGGT CCCCACGCAG ACGGAGTATC 
    TCAGGGACCA CTACATCTGA GAAACCCAAC TCTATGGACA CTGCGAATAC CTCACCCTTC
    AAAGTACCAG GGTTCTTCAG CAAGCGCCTA AAAGGCTCCA TCAAGAGGAC CAAAAGCCAG
    TCAAAGCTTG ACCGAAACAC GAGCTTTCGG CTTCCCTCTC TTCGCAATAC CGATGACAGG
    TCTCGTGGGC TGCCTAAACT AAAAGAGTCC CGTTCTCATG AATCTTTGCT GAGCCCATGC
    AGTGCCGTGG AATGTCTGGA TCTCGGTAGA GGGGAGCCTG TGTCGGTAAA GCCACTCCAT
    AGCAGCATCC TTGGACAAGA CTTCTGCTTT GAGGTTACCT ACTTAAGTGG AAGTAAATGC
    TTCAGTTGTA ACTCTGCTTC AGAGAGAGAT AAGTGGATGG AAAACCTGCG CAGGACAGTT
    CAACCAAATA AGGACAATTG TAGACGAGCT GAAAATGTTC TCCGTTTATG GATTATTGAA
    GCCAAGGACC TTGCCCCTAA AAAGAAATAT TTCTGTGAAC TGTGCCTTGA TGATACCCTC
    TTTGCTCGTA CAACTAGCAA GACCAAAGCA GACAATATTT TCTGGGGTGA ACATTTTGAG
    TTCTACAGCC TTCCACCTCT TCATAGCATC ACAGTTCACA TTTATAAAGA TGTGGAAAAA
    AAGAAGAAAA AAGACAAGAA TAATTATGTA GGGCTGGTCA ACATCCCCAC TGCCAGTGTG
    ACTGGTCGCC AGTTTGTAGA AAAGTGGTAC CCAGTGAGCA CACCTACACC CAACAAAGGA
    AAGACAGGAG GACCTTCTAT TCGGATTAAG TCGCGTTTCC AAACCATCAC CATTCTGCCT
    ATGGAGCAAT ACAAAGAGTT TGCAGAATTT GTCACCAGCA ACTATACCAT GTTATGTTCT
    GTTCTTGAAC CAGTAATCAG TGTGAGAAAT AAAGAAGAGC TAGCCTGTGC CTTAGTGCAC
    ATTCTTCAAA GCACTGGAAG GGCCAAGGAT TTTCTGACGG ACTTGGTGAT GTCTGAGGTG
    GATCGTTGTG GAGAACATGA TGTCTTGATC TTCAGAGAGA ACACCATTGC CACCAAATCC
    ATTGAGGAAT ACCTCAAGTT GGTGGGACAA CAGTATCTTC ACGATGCACT GGGGGAGTTC
    ATCAAAGCGT TGTATGAGTC AGATGAAAAC TGTGAAGTGG ATCCCAGCAA ATGTTCATCC
    AGTGAACTTC TGGACCATCA GAGCAACCTG AAAATGTGCT GTGAGCTGGC TTTCTGCAAG
    ATCATCAACT CTTACTGTGT TTTTCCTCGT GAGCTGAAAG AAGTGTTTGC ATCCTGGAAG
    CAGCAGTGCC TGAACCGCGG CAAGCAGGAC ATCAGCGAGA GGCTCATCAG CGCCTCGCTG
    TTTCTCCGTT TTCTGTGCCC GGCCATCATG TCTCCCAGTC TCTTCAACCT CATGCAGGAG
    TATCCTGATG ACCGCACATC TCGGACTCTG ACTCTCATTG CCAAGGTCAT CCAGAACCTG
    GCCAACTTTG CCAAATTTGG TAACAAGGAG GAATACATGG CATTCATGAA TGATTTTTTA
    GAACATGAAT GGGGTGGAAT GAAGCGCTTT CTTTTGGAGA TCTCTAATCC AGACACCATC
    TCAAACACCC CAGGCTTTGA TGGTTACATT GATCTGGGCC GAGAGCTTTC AGTTTTGCAT
    TCCTTACTAT GGGAAGTAGT TTCCCAACTT GATAAGGGTG AAAATTCCTT CCTACAGGCA
    ACCGTGGCAA AATTGGGACC TCTCCCTCGT GTTCTTGCTG ATATTACCAA ATCTCTGACT
    AATCCTACAC CAATACAACA GCAACTGAGA CGCTTCACTG AGCATAACTC CAGTCCAAAT
    GTCAGTGGAA GCCTCTCCTC TGGGCTGCAG AAAATATTTG AAGACCCTAC TGACAGTGAT
    TTGCACAAAC TAAAATCCCC AAGCCAGGAC AACACAGACA GTTATTTCAG AGGGAAGACA
    TTATTGCTGG TCCAGCAAGC CTCCTCCCAG AGCATGACTT ACTCGGAAAA GGATGAAAAG
    GAAAGTAGCC TCCCCAATGG CCGCAGCATC TCCCTCATGG ACCTTCAGGA GACGCACGCA
    GCCCAAGCAG AGCATCCATC TGTGATACTC GACGTGCCCA TGCGCTTGAC CGGAAGCCAG
    CTCTCCATAA CTCAGGTGGC CAGCATCAAG CAACTGCGGG AGACCCAGAG CACCCCCCAG
    AGTGCACCCC AAGTGCGAAG GCCCCTGCAC CCAGCCTTGA ACCAGCCAGG CAGCCTCCAG
    CCCCTGTCCT TCCAGAACCC TGTCTATCAC CTCAGTAACC CCATTCCTGC AATGCCAAAG
    GCCTCTGTGG ACTCCAGTTT GGAGAACCTA AGCACTGCCA GTTCCAGGAG CCAAAGTAAC
    AGTGAAGACT TCAAACTCAG CGGACCCAGC AACAGCAGCA TGGAGGACTT CACCAAACGG
    AGCACGCAGA GTGAGGACTT CTCCAGGCGC CACACCATGC CCGACAGACA CATACCTCTC
    GCCCTGCCGC GGCAGAACAG CACGGGGCAG ACCCAGCTCC GGAAGATGGA CCCGGCGGGG
    CTGGGCGCCC GAGCCAAAGC CCCGCCGTCC CTGCCGCACA GCGCCTCCCT GCGGAGCACC
    GGGAGCATGT CGGTGGCATC AGCGGCTCTG GTGGCCGAGC CCCTGCAGAA CGGGAGCCGG
    TCCCGACAGC AGTCCTCTTC CTCCAGAGAG AGCCCGGTCC CCAAAGTGAG AGCCATCCAG
    AGGCAGCAGA CCCAGCAGGT TCAGTCACCT GTGGACTCCG CCACAATGTC CCCAGTCGAG
    AGGACAGCAG CCTGGGTCCT GAACAATGGG CAGTATGAAG AGGACGTGGA AGAGACTGAG
    CAAAATCAGG ACGAAGCCAA GCATGCTGAG AAGTACGAAC AGGAAATCAC CAAACTGAAG
    GAGCGCCTGC GCGTGTCCAG CCGGCGACTC GAGGAGTATG AGCGCCGGTT GCTAGTGCAG
    GAGCAGCAGA TGCAGAAGCT GCTGCTGGAG TACAAGGCCC GGCTGGAGGA CAGCGAGGAG
    CGGCTGCGCA GGCAGCAGGA GGAGAAGGAC AGCCAGATGA AGAGCATCAT CAGCAGGCTA
    ATGGCTGTGG AAGAGGAACT GAAGAAGGAT CATGCTGAGA TGCAGGCAGT TATTGATGCA
    AAGCAAAAAA TAATCGATGC ACAGGTCATA AATGGAGATG AGATTATGCA AACAGGAAAA
    ACGGATCGTG TCGCTGGATT CGGCCAACAC CAGACTGATG AGTGCGCTGA CCCAGGTGAA
    GGAACGGTAC AGCATGCAGG TCCGAAATGG CATCTCCCCC ACCAACCCCA CCAAGCTTTC
    CATCACGGAG AATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93020
    Clone ID Related Accession (Same CDS sequence) XM_015466926.1 , XM_015475218.2 , XM_015475218.1
    Accession Version XM_015475218.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3438bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ras GTPase-activating protein nGAP isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104439.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGGAAGCA GTTGTGATAA AGAGGTGCCA GCAGAAAGGT CCCCACGCAG ACGGAGTATC 
    TCAGGGACCA CTACATCTGA GAAACCCAAC TCTATGGACA CTGCGAATAC CTCACCCTTC
    AAAGTACCAG GGTTCTTCAG CAAGCGCCTA AAAGGCTCCA TCAAGAGGAC CAAAAGCCAG
    TCAAAGCTTG ACCGAAACAC GAGCTTTCGG CTTCCCTCTC TTCGCAATAC CGATGACAGG
    TCTCGTGGGC TGCCTAAACT AAAAGAGTCC CGTTCTCATG AATCTTTGCT GAGCCCATGC
    AGTGCCGTGG AATGTCTGGA TCTCGGTAGA GGGGAGCCTG TGTCGGTAAA GCCACTCCAT
    AGCAGCATCC TTGGACAAGA CTTCTGCTTT GAGGTTACCT ACTTAAGTGG AAGTAAATGC
    TTCAGTTGTA ACTCTGCTTC AGAGAGAGAT AAGTGGATGG AAAACCTGCG CAGGACAGTT
    CAACCAAATA AGGACAATTG TAGACGAGCT GAAAATGTTC TCCGTTTATG GATTATTGAA
    GCCAAGGACC TTGCCCCTAA AAAGAAATAT TTCTGTGAAC TGTGCCTTGA TGATACCCTC
    TTTGCTCGTA CAACTAGCAA GACCAAAGCA GACAATATTT TCTGGGGTGA ACATTTTGAG
    TTCTACAGCC TTCCACCTCT TCATAGCATC ACAGTTCACA TTTATAAAGA TGTGGAAAAA
    AAGAAGAAAA AAGACAAGAA TAATTATGTA GGGCTGGTCA ACATCCCCAC TGCCAGTGTG
    ACTGGTCGCC AGTTTGTAGA AAAGTGGTAC CCAGTGAGCA CACCTACACC CAACAAAGGA
    AAGACAGGAG GACCTTCTAT TCGGATTAAG TCGCGTTTCC AAACCATCAC CATTCTGCCT
    ATGGAGCAAT ACAAAGAGTT TGCAGAATTT GTCACCAGCA ACTATACCAT GTTATGTTCT
    GTTCTTGAAC CAGTAATCAG TGTGAGAAAT AAAGAAGAGC TAGCCTGTGC CTTAGTGCAC
    ATTCTTCAAA GCACTGGAAG GGCCAAGGAT TTTCTGACGG ACTTGGTGAT GTCTGAGGTG
    GATCGTTGTG GAGAACATGA TGTCTTGATC TTCAGAGAGA ACACCATTGC CACCAAATCC
    ATTGAGGAAT ACCTCAAGTT GGTGGGACAA CAGTATCTTC ACGATGCACT GGGGGAGTTC
    ATCAAAGCGT TGTATGAGTC AGATGAAAAC TGTGAAGTGG ATCCCAGCAA ATGTTCATCC
    AGTGAACTTC TGGACCATCA GAGCAACCTG AAAATGTGCT GTGAGCTGGC TTTCTGCAAG
    ATCATCAACT CTTACTGTGT TTTTCCTCGT GAGCTGAAAG AAGTGTTTGC ATCCTGGAAG
    CAGCAGTGCC TGAACCGCGG CAAGCAGGAC ATCAGCGAGA GGCTCATCAG CGCCTCGCTG
    TTTCTCCGTT TTCTGTGCCC GGCCATCATG TCTCCCAGTC TCTTCAACCT CATGCAGGAG
    TATCCTGATG ACCGCACATC TCGGACTCTG ACTCTCATTG CCAAGGTCAT CCAGAACCTG
    GCCAACTTTG CCAAATTTGG TAACAAGGAG GAATACATGG CATTCATGAA TGATTTTTTA
    GAACATGAAT GGGGTGGAAT GAAGCGCTTT CTTTTGGAGA TCTCTAATCC AGACACCATC
    TCAAACACCC CAGGCTTTGA TGGTTACATT GATCTGGGCC GAGAGCTTTC AGTTTTGCAT
    TCCTTACTAT GGGAAGTAGT TTCCCAACTT GATAAGGGTG AAAATTCCTT CCTACAGGCA
    ACCGTGGCAA AATTGGGACC TCTCCCTCGT GTTCTTGCTG ATATTACCAA ATCTCTGACT
    AATCCTACAC CAATACAACA GCAACTGAGA CGCTTCACTG AGCATAACTC CAGTCCAAAT
    GTCAGTGGAA GCCTCTCCTC TGGGCTGCAG AAAATATTTG AAGACCCTAC TGACAGTGAT
    TTGCACAAAC TAAAATCCCC AAGCCAGGAC AACACAGACA GTTATTTCAG AGGGAAGACA
    TTATTGCTGG TCCAGCAAGC CTCCTCCCAG AGCATGACTT ACTCGGAAAA GGATGAAAAG
    GAAAGTAGCC TCCCCAATGG CCGCAGCATC TCCCTCATGG ACCTTCAGGA GACGCACGCA
    GCCCAAGCAG AGCATCCATC TGTGATACTC GACGTGCCCA TGCGCTTGAC CGGAAGCCAG
    CTCTCCATAA CTCAGGTGGC CAGCATCAAG CAACTGCGGG AGACCCAGAG CACCCCCCAG
    AGTGCACCCC AAGTGCGAAG GCCCCTGCAC CCAGCCTTGA ACCAGCCAGG CAGCCTCCAG
    CCCCTGTCCT TCCAGAACCC TGTCTATCAC CTCAGTAACC CCATTCCTGC AATGCCAAAG
    GCCTCTGTGG ACTCCAGTTT GGAGAACCTA AGCACTGCCA GTTCCAGGAG CCAAAGTAAC
    AGTGAAGACT TCAAACTCAG CGGACCCAGC AACAGCAGCA TGGAGGACTT CACCAAACGG
    AGCACGCAGA GTGAGGACTT CTCCAGGCGC CACACCATGC CCGACAGACA CATACCTCTC
    GCCCTGCCGC GGCAGAACAG CACGGGGCAG ACCCAGCTCC GGAAGATGGA CCCGGCGGGG
    CTGGGCGCCC GAGCCAAAGC CCCGCCGTCC CTGCCGCACA GCGCCTCCCT GCGGAGCACC
    GGGAGCATGT CGGTGGCATC AGCGGCTCTG GTGGCCGAGC CCCTGCAGAA CGGGAGCCGG
    TCCCGACAGC AGTCCTCTTC CTCCAGAGAG AGCCCGGTCC CCAAAGTGAG AGCCATCCAG
    AGGCAGCAGA CCCAGCAGGT TCAGTCACCT GTGGACTCCG CCACAATGTC CCCAGTCGAG
    AGGACAGCAG CCTGGGTCCT GAACAATGGG CAGTATGAAG AGGACGTGGA AGAGACTGAG
    CAAAATCAGG ACGAAGCCAA GCATGCTGAG AAGTACGAAC AGGAAATCAC CAAACTGAAG
    GAGCGCCTGC GCGTGTCCAG CCGGCGACTC GAGGAGTATG AGCGCCGGTT GCTAGTGCAG
    GAGCAGCAGA TGCAGAAGCT GCTGCTGGAG TACAAGGCCC GGCTGGAGGA CAGCGAGGAG
    CGGCTGCGCA GGCAGCAGGA GGAGAAGGAC AGCCAGATGA AGAGCATCAT CAGCAGGCTA
    ATGGCTGTGG AAGAGGAACT GAAGAAGGAT CATGCTGAGA TGCAGGCAGT TATTGATGCA
    AAGCAAAAAA TAATCGATGC ACAGGTCATA AATGGAGATG AGATTATGCA AACAGGAAAA
    ACGGATCGTG TCGCTGGATT CGGCCAACAC CAGACTGATG AGTGCGCTGA CCCAGGTGAA
    GGAACGGTAC AGCATGCAGG TCCGAAATGG CATCTCCCCC ACCAACCCCA CCAAGCTTTC
    CATCACGGAG AATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330704.1
    CDS68..3505
    Translation

    Target ORF information:

    RefSeq Version XM_015475218.1
    Organism Bos taurus(cattle)
    Definition Bos taurus RAS protein activator like 2 (RASAL2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475218.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGGAAGCA GTTGTGATAA AGAGGTGCCA GCAGAAAGGT CCCCACGCAG ACGGAGTATC 
    TCAGGGACCA CTACATCTGA GAAACCCAAC TCTATGGACA CTGCGAATAC CTCACCCTTC
    AAAGTACCAG GGTTCTTCAG CAAGCGCCTA AAAGGCTCCA TCAAGAGGAC CAAAAGCCAG
    TCAAAGCTTG ACCGAAACAC GAGCTTTCGG CTTCCCTCTC TTCGCAATAC CGATGACAGG
    TCTCGTGGGC TGCCTAAACT AAAAGAGTCC CGTTCTCATG AATCTTTGCT GAGCCCATGC
    AGTGCCGTGG AATGTCTGGA TCTCGGTAGA GGGGAGCCTG TGTCGGTAAA GCCACTCCAT
    AGCAGCATCC TTGGACAAGA CTTCTGCTTT GAGGTTACCT ACTTAAGTGG AAGTAAATGC
    TTCAGTTGTA ACTCTGCTTC AGAGAGAGAT AAGTGGATGG AAAACCTGCG CAGGACAGTT
    CAACCAAATA AGGACAATTG TAGACGAGCT GAAAATGTTC TCCGTTTATG GATTATTGAA
    GCCAAGGACC TTGCCCCTAA AAAGAAATAT TTCTGTGAAC TGTGCCTTGA TGATACCCTC
    TTTGCTCGTA CAACTAGCAA GACCAAAGCA GACAATATTT TCTGGGGTGA ACATTTTGAG
    TTCTACAGCC TTCCACCTCT TCATAGCATC ACAGTTCACA TTTATAAAGA TGTGGAAAAA
    AAGAAGAAAA AAGACAAGAA TAATTATGTA GGGCTGGTCA ACATCCCCAC TGCCAGTGTG
    ACTGGTCGCC AGTTTGTAGA AAAGTGGTAC CCAGTGAGCA CACCTACACC CAACAAAGGA
    AAGACAGGAG GACCTTCTAT TCGGATTAAG TCGCGTTTCC AAACCATCAC CATTCTGCCT
    ATGGAGCAAT ACAAAGAGTT TGCAGAATTT GTCACCAGCA ACTATACCAT GTTATGTTCT
    GTTCTTGAAC CAGTAATCAG TGTGAGAAAT AAAGAAGAGC TAGCCTGTGC CTTAGTGCAC
    ATTCTTCAAA GCACTGGAAG GGCCAAGGAT TTTCTGACGG ACTTGGTGAT GTCTGAGGTG
    GATCGTTGTG GAGAACATGA TGTCTTGATC TTCAGAGAGA ACACCATTGC CACCAAATCC
    ATTGAGGAAT ACCTCAAGTT GGTGGGACAA CAGTATCTTC ACGATGCACT GGGGGAGTTC
    ATCAAAGCGT TGTATGAGTC AGATGAAAAC TGTGAAGTGG ATCCCAGCAA ATGTTCATCC
    AGTGAACTTC TGGACCATCA GAGCAACCTG AAAATGTGCT GTGAGCTGGC TTTCTGCAAG
    ATCATCAACT CTTACTGTGT TTTTCCTCGT GAGCTGAAAG AAGTGTTTGC ATCCTGGAAG
    CAGCAGTGCC TGAACCGCGG CAAGCAGGAC ATCAGCGAGA GGCTCATCAG CGCCTCGCTG
    TTTCTCCGTT TTCTGTGCCC GGCCATCATG TCTCCCAGTC TCTTCAACCT CATGCAGGAG
    TATCCTGATG ACCGCACATC TCGGACTCTG ACTCTCATTG CCAAGGTCAT CCAGAACCTG
    GCCAACTTTG CCAAATTTGG TAACAAGGAG GAATACATGG CATTCATGAA TGATTTTTTA
    GAACATGAAT GGGGTGGAAT GAAGCGCTTT CTTTTGGAGA TCTCTAATCC AGACACCATC
    TCAAACACCC CAGGCTTTGA TGGTTACATT GATCTGGGCC GAGAGCTTTC AGTTTTGCAT
    TCCTTACTAT GGGAAGTAGT TTCCCAACTT GATAAGGGTG AAAATTCCTT CCTACAGGCA
    ACCGTGGCAA AATTGGGACC TCTCCCTCGT GTTCTTGCTG ATATTACCAA ATCTCTGACT
    AATCCTACAC CAATACAACA GCAACTGAGA CGCTTCACTG AGCATAACTC CAGTCCAAAT
    GTCAGTGGAA GCCTCTCCTC TGGGCTGCAG AAAATATTTG AAGACCCTAC TGACAGTGAT
    TTGCACAAAC TAAAATCCCC AAGCCAGGAC AACACAGACA GTTATTTCAG AGGGAAGACA
    TTATTGCTGG TCCAGCAAGC CTCCTCCCAG AGCATGACTT ACTCGGAAAA GGATGAAAAG
    GAAAGTAGCC TCCCCAATGG CCGCAGCATC TCCCTCATGG ACCTTCAGGA GACGCACGCA
    GCCCAAGCAG AGCATCCATC TGTGATACTC GACGTGCCCA TGCGCTTGAC CGGAAGCCAG
    CTCTCCATAA CTCAGGTGGC CAGCATCAAG CAACTGCGGG AGACCCAGAG CACCCCCCAG
    AGTGCACCCC AAGTGCGAAG GCCCCTGCAC CCAGCCTTGA ACCAGCCAGG CAGCCTCCAG
    CCCCTGTCCT TCCAGAACCC TGTCTATCAC CTCAGTAACC CCATTCCTGC AATGCCAAAG
    GCCTCTGTGG ACTCCAGTTT GGAGAACCTA AGCACTGCCA GTTCCAGGAG CCAAAGTAAC
    AGTGAAGACT TCAAACTCAG CGGACCCAGC AACAGCAGCA TGGAGGACTT CACCAAACGG
    AGCACGCAGA GTGAGGACTT CTCCAGGCGC CACACCATGC CCGACAGACA CATACCTCTC
    GCCCTGCCGC GGCAGAACAG CACGGGGCAG ACCCAGCTCC GGAAGATGGA CCCGGCGGGG
    CTGGGCGCCC GAGCCAAAGC CCCGCCGTCC CTGCCGCACA GCGCCTCCCT GCGGAGCACC
    GGGAGCATGT CGGTGGCATC AGCGGCTCTG GTGGCCGAGC CCCTGCAGAA CGGGAGCCGG
    TCCCGACAGC AGTCCTCTTC CTCCAGAGAG AGCCCGGTCC CCAAAGTGAG AGCCATCCAG
    AGGCAGCAGA CCCAGCAGGT TCAGTCACCT GTGGACTCCG CCACAATGTC CCCAGTCGAG
    AGGACAGCAG CCTGGGTCCT GAACAATGGG CAGTATGAAG AGGACGTGGA AGAGACTGAG
    CAAAATCAGG ACGAAGCCAA GCATGCTGAG AAGTACGAAC AGGAAATCAC CAAACTGAAG
    GAGCGCCTGC GCGTGTCCAG CCGGCGACTC GAGGAGTATG AGCGCCGGTT GCTAGTGCAG
    GAGCAGCAGA TGCAGAAGCT GCTGCTGGAG TACAAGGCCC GGCTGGAGGA CAGCGAGGAG
    CGGCTGCGCA GGCAGCAGGA GGAGAAGGAC AGCCAGATGA AGAGCATCAT CAGCAGGCTA
    ATGGCTGTGG AAGAGGAACT GAAGAAGGAT CATGCTGAGA TGCAGGCAGT TATTGATGCA
    AAGCAAAAAA TAATCGATGC ACAGGTCATA AATGGAGATG AGATTATGCA AACAGGAAAA
    ACGGATCGTG TCGCTGGATT CGGCCAACAC CAGACTGATG AGTGCGCTGA CCCAGGTGAA
    GGAACGGTAC AGCATGCAGG TCCGAAATGG CATCTCCCCC ACCAACCCCA CCAAGCTTTC
    CATCACGGAG AATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa08813
    Clone ID Related Accession (Same CDS sequence) XM_005217242.3 , XM_612667.8 , XM_005217242.4
    Accession Version XM_005217242.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3429bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ras GTPase-activating protein nGAP isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104439.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005217242.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCAGACCC CAGAGGTGCC AGCAGAAAGG TCCCCACGCA GACGGAGTAT CTCAGGGACC 
    ACTACATCTG AGAAACCCAA CTCTATGGAC ACTGCGAATA CCTCACCCTT CAAAGTACCA
    GGGTTCTTCA GCAAGCGCCT AAAAGGCTCC ATCAAGAGGA CCAAAAGCCA GTCAAAGCTT
    GACCGAAACA CGAGCTTTCG GCTTCCCTCT CTTCGCAATA CCGATGACAG GTCTCGTGGG
    CTGCCTAAAC TAAAAGAGTC CCGTTCTCAT GAATCTTTGC TGAGCCCATG CAGTGCCGTG
    GAATGTCTGG ATCTCGGTAG AGGGGAGCCT GTGTCGGTAA AGCCACTCCA TAGCAGCATC
    CTTGGACAAG ACTTCTGCTT TGAGGTTACC TACTTAAGTG GAAGTAAATG CTTCAGTTGT
    AACTCTGCTT CAGAGAGAGA TAAGTGGATG GAAAACCTGC GCAGGACAGT TCAACCAAAT
    AAGGACAATT GTAGACGAGC TGAAAATGTT CTCCGTTTAT GGATTATTGA AGCCAAGGAC
    CTTGCCCCTA AAAAGAAATA TTTCTGTGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT
    ACAACTAGCA AGACCAAAGC AGACAATATT TTCTGGGGTG AACATTTTGA GTTCTACAGC
    CTTCCACCTC TTCATAGCAT CACAGTTCAC ATTTATAAAG ATGTGGAAAA AAAGAAGAAA
    AAAGACAAGA ATAATTATGT AGGGCTGGTC AACATCCCCA CTGCCAGTGT GACTGGTCGC
    CAGTTTGTAG AAAAGTGGTA CCCAGTGAGC ACACCTACAC CCAACAAAGG AAAGACAGGA
    GGACCTTCTA TTCGGATTAA GTCGCGTTTC CAAACCATCA CCATTCTGCC TATGGAGCAA
    TACAAAGAGT TTGCAGAATT TGTCACCAGC AACTATACCA TGTTATGTTC TGTTCTTGAA
    CCAGTAATCA GTGTGAGAAA TAAAGAAGAG CTAGCCTGTG CCTTAGTGCA CATTCTTCAA
    AGCACTGGAA GGGCCAAGGA TTTTCTGACG GACTTGGTGA TGTCTGAGGT GGATCGTTGT
    GGAGAACATG ATGTCTTGAT CTTCAGAGAG AACACCATTG CCACCAAATC CATTGAGGAA
    TACCTCAAGT TGGTGGGACA ACAGTATCTT CACGATGCAC TGGGGGAGTT CATCAAAGCG
    TTGTATGAGT CAGATGAAAA CTGTGAAGTG GATCCCAGCA AATGTTCATC CAGTGAACTT
    CTGGACCATC AGAGCAACCT GAAAATGTGC TGTGAGCTGG CTTTCTGCAA GATCATCAAC
    TCTTACTGTG TTTTTCCTCG TGAGCTGAAA GAAGTGTTTG CATCCTGGAA GCAGCAGTGC
    CTGAACCGCG GCAAGCAGGA CATCAGCGAG AGGCTCATCA GCGCCTCGCT GTTTCTCCGT
    TTTCTGTGCC CGGCCATCAT GTCTCCCAGT CTCTTCAACC TCATGCAGGA GTATCCTGAT
    GACCGCACAT CTCGGACTCT GACTCTCATT GCCAAGGTCA TCCAGAACCT GGCCAACTTT
    GCCAAATTTG GTAACAAGGA GGAATACATG GCATTCATGA ATGATTTTTT AGAACATGAA
    TGGGGTGGAA TGAAGCGCTT TCTTTTGGAG ATCTCTAATC CAGACACCAT CTCAAACACC
    CCAGGCTTTG ATGGTTACAT TGATCTGGGC CGAGAGCTTT CAGTTTTGCA TTCCTTACTA
    TGGGAAGTAG TTTCCCAACT TGATAAGGGT GAAAATTCCT TCCTACAGGC AACCGTGGCA
    AAATTGGGAC CTCTCCCTCG TGTTCTTGCT GATATTACCA AATCTCTGAC TAATCCTACA
    CCAATACAAC AGCAACTGAG ACGCTTCACT GAGCATAACT CCAGTCCAAA TGTCAGTGGA
    AGCCTCTCCT CTGGGCTGCA GAAAATATTT GAAGACCCTA CTGACAGTGA TTTGCACAAA
    CTAAAATCCC CAAGCCAGGA CAACACAGAC AGTTATTTCA GAGGGAAGAC ATTATTGCTG
    GTCCAGCAAG CCTCCTCCCA GAGCATGACT TACTCGGAAA AGGATGAAAA GGAAAGTAGC
    CTCCCCAATG GCCGCAGCAT CTCCCTCATG GACCTTCAGG AGACGCACGC AGCCCAAGCA
    GAGCATCCAT CTGTGATACT CGACGTGCCC ATGCGCTTGA CCGGAAGCCA GCTCTCCATA
    ACTCAGGTGG CCAGCATCAA GCAACTGCGG GAGACCCAGA GCACCCCCCA GAGTGCACCC
    CAAGTGCGAA GGCCCCTGCA CCCAGCCTTG AACCAGCCAG GCAGCCTCCA GCCCCTGTCC
    TTCCAGAACC CTGTCTATCA CCTCAGTAAC CCCATTCCTG CAATGCCAAA GGCCTCTGTG
    GACTCCAGTT TGGAGAACCT AAGCACTGCC AGTTCCAGGA GCCAAAGTAA CAGTGAAGAC
    TTCAAACTCA GCGGACCCAG CAACAGCAGC ATGGAGGACT TCACCAAACG GAGCACGCAG
    AGTGAGGACT TCTCCAGGCG CCACACCATG CCCGACAGAC ACATACCTCT CGCCCTGCCG
    CGGCAGAACA GCACGGGGCA GACCCAGCTC CGGAAGATGG ACCCGGCGGG GCTGGGCGCC
    CGAGCCAAAG CCCCGCCGTC CCTGCCGCAC AGCGCCTCCC TGCGGAGCAC CGGGAGCATG
    TCGGTGGCAT CAGCGGCTCT GGTGGCCGAG CCCCTGCAGA ACGGGAGCCG GTCCCGACAG
    CAGTCCTCTT CCTCCAGAGA GAGCCCGGTC CCCAAAGTGA GAGCCATCCA GAGGCAGCAG
    ACCCAGCAGG TTCAGTCACC TGTGGACTCC GCCACAATGT CCCCAGTCGA GAGGACAGCA
    GCCTGGGTCC TGAACAATGG GCAGTATGAA GAGGACGTGG AAGAGACTGA GCAAAATCAG
    GACGAAGCCA AGCATGCTGA GAAGTACGAA CAGGAAATCA CCAAACTGAA GGAGCGCCTG
    CGCGTGTCCA GCCGGCGACT CGAGGAGTAT GAGCGCCGGT TGCTAGTGCA GGAGCAGCAG
    ATGCAGAAGC TGCTGCTGGA GTACAAGGCC CGGCTGGAGG ACAGCGAGGA GCGGCTGCGC
    AGGCAGCAGG AGGAGAAGGA CAGCCAGATG AAGAGCATCA TCAGCAGGCT AATGGCTGTG
    GAAGAGGAAC TGAAGAAGGA TCATGCTGAG ATGCAGGCAG TTATTGATGC AAAGCAAAAA
    ATAATCGATG CACAGGTCAT AAATGGAGAT GAGATTATGC AAACAGGAAA AACGGATCGT
    GTCGCTGGAT TCGGCCAACA CCAGACTGAT GAGTGCGCTG ACCCAGGTGA AGGAACGGTA
    CAGCATGCAG GTCCGAAATG GCATCTCCCC CACCAACCCC ACCAAGCTTT CCATCACGGA
    GAATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005217299.1
    CDS163..3591
    Translation

    Target ORF information:

    RefSeq Version XM_005217242.3
    Organism Bos taurus(cattle)
    Definition Bos taurus RAS protein activator like 2 (RASAL2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005217242.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCAGACCC CAGAGGTGCC AGCAGAAAGG TCCCCACGCA GACGGAGTAT CTCAGGGACC 
    ACTACATCTG AGAAACCCAA CTCTATGGAC ACTGCGAATA CCTCACCCTT CAAAGTACCA
    GGGTTCTTCA GCAAGCGCCT AAAAGGCTCC ATCAAGAGGA CCAAAAGCCA GTCAAAGCTT
    GACCGAAACA CGAGCTTTCG GCTTCCCTCT CTTCGCAATA CCGATGACAG GTCTCGTGGG
    CTGCCTAAAC TAAAAGAGTC CCGTTCTCAT GAATCTTTGC TGAGCCCATG CAGTGCCGTG
    GAATGTCTGG ATCTCGGTAG AGGGGAGCCT GTGTCGGTAA AGCCACTCCA TAGCAGCATC
    CTTGGACAAG ACTTCTGCTT TGAGGTTACC TACTTAAGTG GAAGTAAATG CTTCAGTTGT
    AACTCTGCTT CAGAGAGAGA TAAGTGGATG GAAAACCTGC GCAGGACAGT TCAACCAAAT
    AAGGACAATT GTAGACGAGC TGAAAATGTT CTCCGTTTAT GGATTATTGA AGCCAAGGAC
    CTTGCCCCTA AAAAGAAATA TTTCTGTGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT
    ACAACTAGCA AGACCAAAGC AGACAATATT TTCTGGGGTG AACATTTTGA GTTCTACAGC
    CTTCCACCTC TTCATAGCAT CACAGTTCAC ATTTATAAAG ATGTGGAAAA AAAGAAGAAA
    AAAGACAAGA ATAATTATGT AGGGCTGGTC AACATCCCCA CTGCCAGTGT GACTGGTCGC
    CAGTTTGTAG AAAAGTGGTA CCCAGTGAGC ACACCTACAC CCAACAAAGG AAAGACAGGA
    GGACCTTCTA TTCGGATTAA GTCGCGTTTC CAAACCATCA CCATTCTGCC TATGGAGCAA
    TACAAAGAGT TTGCAGAATT TGTCACCAGC AACTATACCA TGTTATGTTC TGTTCTTGAA
    CCAGTAATCA GTGTGAGAAA TAAAGAAGAG CTAGCCTGTG CCTTAGTGCA CATTCTTCAA
    AGCACTGGAA GGGCCAAGGA TTTTCTGACG GACTTGGTGA TGTCTGAGGT GGATCGTTGT
    GGAGAACATG ATGTCTTGAT CTTCAGAGAG AACACCATTG CCACCAAATC CATTGAGGAA
    TACCTCAAGT TGGTGGGACA ACAGTATCTT CACGATGCAC TGGGGGAGTT CATCAAAGCG
    TTGTATGAGT CAGATGAAAA CTGTGAAGTG GATCCCAGCA AATGTTCATC CAGTGAACTT
    CTGGACCATC AGAGCAACCT GAAAATGTGC TGTGAGCTGG CTTTCTGCAA GATCATCAAC
    TCTTACTGTG TTTTTCCTCG TGAGCTGAAA GAAGTGTTTG CATCCTGGAA GCAGCAGTGC
    CTGAACCGCG GCAAGCAGGA CATCAGCGAG AGGCTCATCA GCGCCTCGCT GTTTCTCCGT
    TTTCTGTGCC CGGCCATCAT GTCTCCCAGT CTCTTCAACC TCATGCAGGA GTATCCTGAT
    GACCGCACAT CTCGGACTCT GACTCTCATT GCCAAGGTCA TCCAGAACCT GGCCAACTTT
    GCCAAATTTG GTAACAAGGA GGAATACATG GCATTCATGA ATGATTTTTT AGAACATGAA
    TGGGGTGGAA TGAAGCGCTT TCTTTTGGAG ATCTCTAATC CAGACACCAT CTCAAACACC
    CCAGGCTTTG ATGGTTACAT TGATCTGGGC CGAGAGCTTT CAGTTTTGCA TTCCTTACTA
    TGGGAAGTAG TTTCCCAACT TGATAAGGGT GAAAATTCCT TCCTACAGGC AACCGTGGCA
    AAATTGGGAC CTCTCCCTCG TGTTCTTGCT GATATTACCA AATCTCTGAC TAATCCTACA
    CCAATACAAC AGCAACTGAG ACGCTTCACT GAGCATAACT CCAGTCCAAA TGTCAGTGGA
    AGCCTCTCCT CTGGGCTGCA GAAAATATTT GAAGACCCTA CTGACAGTGA TTTGCACAAA
    CTAAAATCCC CAAGCCAGGA CAACACAGAC AGTTATTTCA GAGGGAAGAC ATTATTGCTG
    GTCCAGCAAG CCTCCTCCCA GAGCATGACT TACTCGGAAA AGGATGAAAA GGAAAGTAGC
    CTCCCCAATG GCCGCAGCAT CTCCCTCATG GACCTTCAGG AGACGCACGC AGCCCAAGCA
    GAGCATCCAT CTGTGATACT CGACGTGCCC ATGCGCTTGA CCGGAAGCCA GCTCTCCATA
    ACTCAGGTGG CCAGCATCAA GCAACTGCGG GAGACCCAGA GCACCCCCCA GAGTGCACCC
    CAAGTGCGAA GGCCCCTGCA CCCAGCCTTG AACCAGCCAG GCAGCCTCCA GCCCCTGTCC
    TTCCAGAACC CTGTCTATCA CCTCAGTAAC CCCATTCCTG CAATGCCAAA GGCCTCTGTG
    GACTCCAGTT TGGAGAACCT AAGCACTGCC AGTTCCAGGA GCCAAAGTAA CAGTGAAGAC
    TTCAAACTCA GCGGACCCAG CAACAGCAGC ATGGAGGACT TCACCAAACG GAGCACGCAG
    AGTGAGGACT TCTCCAGGCG CCACACCATG CCCGACAGAC ACATACCTCT CGCCCTGCCG
    CGGCAGAACA GCACGGGGCA GACCCAGCTC CGGAAGATGG ACCCGGCGGG GCTGGGCGCC
    CGAGCCAAAG CCCCGCCGTC CCTGCCGCAC AGCGCCTCCC TGCGGAGCAC CGGGAGCATG
    TCGGTGGCAT CAGCGGCTCT GGTGGCCGAG CCCCTGCAGA ACGGGAGCCG GTCCCGACAG
    CAGTCCTCTT CCTCCAGAGA GAGCCCGGTC CCCAAAGTGA GAGCCATCCA GAGGCAGCAG
    ACCCAGCAGG TTCAGTCACC TGTGGACTCC GCCACAATGT CCCCAGTCGA GAGGACAGCA
    GCCTGGGTCC TGAACAATGG GCAGTATGAA GAGGACGTGG AAGAGACTGA GCAAAATCAG
    GACGAAGCCA AGCATGCTGA GAAGTACGAA CAGGAAATCA CCAAACTGAA GGAGCGCCTG
    CGCGTGTCCA GCCGGCGACT CGAGGAGTAT GAGCGCCGGT TGCTAGTGCA GGAGCAGCAG
    ATGCAGAAGC TGCTGCTGGA GTACAAGGCC CGGCTGGAGG ACAGCGAGGA GCGGCTGCGC
    AGGCAGCAGG AGGAGAAGGA CAGCCAGATG AAGAGCATCA TCAGCAGGCT AATGGCTGTG
    GAAGAGGAAC TGAAGAAGGA TCATGCTGAG ATGCAGGCAG TTATTGATGC AAAGCAAAAA
    ATAATCGATG CACAGGTCAT AAATGGAGAT GAGATTATGC AAACAGGAAA AACGGATCGT
    GTCGCTGGAT TCGGCCAACA CCAGACTGAT GAGTGCGCTG ACCCAGGTGA AGGAACGGTA
    CAGCATGCAG GTCCGAAATG GCATCTCCCC CACCAACCCC ACCAAGCTTT CCATCACGGA
    GAATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa08813
    Clone ID Related Accession (Same CDS sequence) XM_005217242.3 , XM_612667.8 , XM_005217242.4
    Accession Version XM_612667.8 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3429bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ras GTPase-activating protein nGAP isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646324.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_612667.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCAGACCC CAGAGGTGCC AGCAGAAAGG TCCCCACGCA GACGGAGTAT CTCAGGGACC 
    ACTACATCTG AGAAACCCAA CTCTATGGAC ACTGCGAATA CCTCACCCTT CAAAGTACCA
    GGGTTCTTCA GCAAGCGCCT AAAAGGCTCC ATCAAGAGGA CCAAAAGCCA GTCAAAGCTT
    GACCGAAACA CGAGCTTTCG GCTTCCCTCT CTTCGCAATA CCGATGACAG GTCTCGTGGG
    CTGCCTAAAC TAAAAGAGTC CCGTTCTCAT GAATCTTTGC TGAGCCCATG CAGTGCCGTG
    GAATGTCTGG ATCTCGGTAG AGGGGAGCCT GTGTCGGTAA AGCCACTCCA TAGCAGCATC
    CTTGGACAAG ACTTCTGCTT TGAGGTTACC TACTTAAGTG GAAGTAAATG CTTCAGTTGT
    AACTCTGCTT CAGAGAGAGA TAAGTGGATG GAAAACCTGC GCAGGACAGT TCAACCAAAT
    AAGGACAATT GTAGACGAGC TGAAAATGTT CTCCGTTTAT GGATTATTGA AGCCAAGGAC
    CTTGCCCCTA AAAAGAAATA TTTCTGTGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT
    ACAACTAGCA AGACCAAAGC AGACAATATT TTCTGGGGTG AACATTTTGA GTTCTACAGC
    CTTCCACCTC TTCATAGCAT CACAGTTCAC ATTTATAAAG ATGTGGAAAA AAAGAAGAAA
    AAAGACAAGA ATAATTATGT AGGGCTGGTC AACATCCCCA CTGCCAGTGT GACTGGTCGC
    CAGTTTGTAG AAAAGTGGTA CCCAGTGAGC ACACCTACAC CCAACAAAGG AAAGACAGGA
    GGACCTTCTA TTCGGATTAA GTCGCGTTTC CAAACCATCA CCATTCTGCC TATGGAGCAA
    TACAAAGAGT TTGCAGAATT TGTCACCAGC AACTATACCA TGTTATGTTC TGTTCTTGAA
    CCAGTAATCA GTGTGAGAAA TAAAGAAGAG CTAGCCTGTG CCTTAGTGCA CATTCTTCAA
    AGCACTGGAA GGGCCAAGGA TTTTCTGACG GACTTGGTGA TGTCTGAGGT GGATCGTTGT
    GGAGAACATG ATGTCTTGAT CTTCAGAGAG AACACCATTG CCACCAAATC CATTGAGGAA
    TACCTCAAGT TGGTGGGACA ACAGTATCTT CACGATGCAC TGGGGGAGTT CATCAAAGCG
    TTGTATGAGT CAGATGAAAA CTGTGAAGTG GATCCCAGCA AATGTTCATC CAGTGAACTT
    CTGGACCATC AGAGCAACCT GAAAATGTGC TGTGAGCTGG CTTTCTGCAA GATCATCAAC
    TCTTACTGTG TTTTTCCTCG TGAGCTGAAA GAAGTGTTTG CATCCTGGAA GCAGCAGTGC
    CTGAACCGCG GCAAGCAGGA CATCAGCGAG AGGCTCATCA GCGCCTCGCT GTTTCTCCGT
    TTTCTGTGCC CGGCCATCAT GTCTCCCAGT CTCTTCAACC TCATGCAGGA GTATCCTGAT
    GACCGCACAT CTCGGACTCT GACTCTCATT GCCAAGGTCA TCCAGAACCT GGCCAACTTT
    GCCAAATTTG GTAACAAGGA GGAATACATG GCATTCATGA ATGATTTTTT AGAACATGAA
    TGGGGTGGAA TGAAGCGCTT TCTTTTGGAG ATCTCTAATC CAGACACCAT CTCAAACACC
    CCAGGCTTTG ATGGTTACAT TGATCTGGGC CGAGAGCTTT CAGTTTTGCA TTCCTTACTA
    TGGGAAGTAG TTTCCCAACT TGATAAGGGT GAAAATTCCT TCCTACAGGC AACCGTGGCA
    AAATTGGGAC CTCTCCCTCG TGTTCTTGCT GATATTACCA AATCTCTGAC TAATCCTACA
    CCAATACAAC AGCAACTGAG ACGCTTCACT GAGCATAACT CCAGTCCAAA TGTCAGTGGA
    AGCCTCTCCT CTGGGCTGCA GAAAATATTT GAAGACCCTA CTGACAGTGA TTTGCACAAA
    CTAAAATCCC CAAGCCAGGA CAACACAGAC AGTTATTTCA GAGGGAAGAC ATTATTGCTG
    GTCCAGCAAG CCTCCTCCCA GAGCATGACT TACTCGGAAA AGGATGAAAA GGAAAGTAGC
    CTCCCCAATG GCCGCAGCAT CTCCCTCATG GACCTTCAGG AGACGCACGC AGCCCAAGCA
    GAGCATCCAT CTGTGATACT CGACGTGCCC ATGCGCTTGA CCGGAAGCCA GCTCTCCATA
    ACTCAGGTGG CCAGCATCAA GCAACTGCGG GAGACCCAGA GCACCCCCCA GAGTGCACCC
    CAAGTGCGAA GGCCCCTGCA CCCAGCCTTG AACCAGCCAG GCAGCCTCCA GCCCCTGTCC
    TTCCAGAACC CTGTCTATCA CCTCAGTAAC CCCATTCCTG CAATGCCAAA GGCCTCTGTG
    GACTCCAGTT TGGAGAACCT AAGCACTGCC AGTTCCAGGA GCCAAAGTAA CAGTGAAGAC
    TTCAAACTCA GCGGACCCAG CAACAGCAGC ATGGAGGACT TCACCAAACG GAGCACGCAG
    AGTGAGGACT TCTCCAGGCG CCACACCATG CCCGACAGAC ACATACCTCT CGCCCTGCCG
    CGGCAGAACA GCACGGGGCA GACCCAGCTC CGGAAGATGG ACCCGGCGGG GCTGGGCGCC
    CGAGCCAAAG CCCCGCCGTC CCTGCCGCAC AGCGCCTCCC TGCGGAGCAC CGGGAGCATG
    TCGGTGGCAT CAGCGGCTCT GGTGGCCGAG CCCCTGCAGA ACGGGAGCCG GTCCCGACAG
    CAGTCCTCTT CCTCCAGAGA GAGCCCGGTC CCCAAAGTGA GAGCCATCCA GAGGCAGCAG
    ACCCAGCAGG TTCAGTCACC TGTGGACTCC GCCACAATGT CCCCAGTCGA GAGGACAGCA
    GCCTGGGTCC TGAACAATGG GCAGTATGAA GAGGACGTGG AAGAGACTGA GCAAAATCAG
    GACGAAGCCA AGCATGCTGA GAAGTACGAA CAGGAAATCA CCAAACTGAA GGAGCGCCTG
    CGCGTGTCCA GCCGGCGACT CGAGGAGTAT GAGCGCCGGT TGCTAGTGCA GGAGCAGCAG
    ATGCAGAAGC TGCTGCTGGA GTACAAGGCC CGGCTGGAGG ACAGCGAGGA GCGGCTGCGC
    AGGCAGCAGG AGGAGAAGGA CAGCCAGATG AAGAGCATCA TCAGCAGGCT AATGGCTGTG
    GAAGAGGAAC TGAAGAAGGA TCATGCTGAG ATGCAGGCAG TTATTGATGC AAAGCAAAAA
    ATAATCGATG CACAGGTCAT AAATGGAGAT GAGATTATGC AAACAGGAAA AACGGATCGT
    GTCGCTGGAT TCGGCCAACA CCAGACTGAT GAGTGCGCTG ACCCAGGTGA AGGAACGGTA
    CAGCATGCAG GTCCGAAATG GCATCTCCCC CACCAACCCC ACCAAGCTTT CCATCACGGA
    GAATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_612667.6
    CDS164..3592
    Translation

    Target ORF information:

    RefSeq Version XM_612667.8
    Organism Bos taurus(cattle)
    Definition Bos taurus RAS protein activator like 2 (RASAL2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_612667.8

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCAGACCC CAGAGGTGCC AGCAGAAAGG TCCCCACGCA GACGGAGTAT CTCAGGGACC 
    ACTACATCTG AGAAACCCAA CTCTATGGAC ACTGCGAATA CCTCACCCTT CAAAGTACCA
    GGGTTCTTCA GCAAGCGCCT AAAAGGCTCC ATCAAGAGGA CCAAAAGCCA GTCAAAGCTT
    GACCGAAACA CGAGCTTTCG GCTTCCCTCT CTTCGCAATA CCGATGACAG GTCTCGTGGG
    CTGCCTAAAC TAAAAGAGTC CCGTTCTCAT GAATCTTTGC TGAGCCCATG CAGTGCCGTG
    GAATGTCTGG ATCTCGGTAG AGGGGAGCCT GTGTCGGTAA AGCCACTCCA TAGCAGCATC
    CTTGGACAAG ACTTCTGCTT TGAGGTTACC TACTTAAGTG GAAGTAAATG CTTCAGTTGT
    AACTCTGCTT CAGAGAGAGA TAAGTGGATG GAAAACCTGC GCAGGACAGT TCAACCAAAT
    AAGGACAATT GTAGACGAGC TGAAAATGTT CTCCGTTTAT GGATTATTGA AGCCAAGGAC
    CTTGCCCCTA AAAAGAAATA TTTCTGTGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT
    ACAACTAGCA AGACCAAAGC AGACAATATT TTCTGGGGTG AACATTTTGA GTTCTACAGC
    CTTCCACCTC TTCATAGCAT CACAGTTCAC ATTTATAAAG ATGTGGAAAA AAAGAAGAAA
    AAAGACAAGA ATAATTATGT AGGGCTGGTC AACATCCCCA CTGCCAGTGT GACTGGTCGC
    CAGTTTGTAG AAAAGTGGTA CCCAGTGAGC ACACCTACAC CCAACAAAGG AAAGACAGGA
    GGACCTTCTA TTCGGATTAA GTCGCGTTTC CAAACCATCA CCATTCTGCC TATGGAGCAA
    TACAAAGAGT TTGCAGAATT TGTCACCAGC AACTATACCA TGTTATGTTC TGTTCTTGAA
    CCAGTAATCA GTGTGAGAAA TAAAGAAGAG CTAGCCTGTG CCTTAGTGCA CATTCTTCAA
    AGCACTGGAA GGGCCAAGGA TTTTCTGACG GACTTGGTGA TGTCTGAGGT GGATCGTTGT
    GGAGAACATG ATGTCTTGAT CTTCAGAGAG AACACCATTG CCACCAAATC CATTGAGGAA
    TACCTCAAGT TGGTGGGACA ACAGTATCTT CACGATGCAC TGGGGGAGTT CATCAAAGCG
    TTGTATGAGT CAGATGAAAA CTGTGAAGTG GATCCCAGCA AATGTTCATC CAGTGAACTT
    CTGGACCATC AGAGCAACCT GAAAATGTGC TGTGAGCTGG CTTTCTGCAA GATCATCAAC
    TCTTACTGTG TTTTTCCTCG TGAGCTGAAA GAAGTGTTTG CATCCTGGAA GCAGCAGTGC
    CTGAACCGCG GCAAGCAGGA CATCAGCGAG AGGCTCATCA GCGCCTCGCT GTTTCTCCGT
    TTTCTGTGCC CGGCCATCAT GTCTCCCAGT CTCTTCAACC TCATGCAGGA GTATCCTGAT
    GACCGCACAT CTCGGACTCT GACTCTCATT GCCAAGGTCA TCCAGAACCT GGCCAACTTT
    GCCAAATTTG GTAACAAGGA GGAATACATG GCATTCATGA ATGATTTTTT AGAACATGAA
    TGGGGTGGAA TGAAGCGCTT TCTTTTGGAG ATCTCTAATC CAGACACCAT CTCAAACACC
    CCAGGCTTTG ATGGTTACAT TGATCTGGGC CGAGAGCTTT CAGTTTTGCA TTCCTTACTA
    TGGGAAGTAG TTTCCCAACT TGATAAGGGT GAAAATTCCT TCCTACAGGC AACCGTGGCA
    AAATTGGGAC CTCTCCCTCG TGTTCTTGCT GATATTACCA AATCTCTGAC TAATCCTACA
    CCAATACAAC AGCAACTGAG ACGCTTCACT GAGCATAACT CCAGTCCAAA TGTCAGTGGA
    AGCCTCTCCT CTGGGCTGCA GAAAATATTT GAAGACCCTA CTGACAGTGA TTTGCACAAA
    CTAAAATCCC CAAGCCAGGA CAACACAGAC AGTTATTTCA GAGGGAAGAC ATTATTGCTG
    GTCCAGCAAG CCTCCTCCCA GAGCATGACT TACTCGGAAA AGGATGAAAA GGAAAGTAGC
    CTCCCCAATG GCCGCAGCAT CTCCCTCATG GACCTTCAGG AGACGCACGC AGCCCAAGCA
    GAGCATCCAT CTGTGATACT CGACGTGCCC ATGCGCTTGA CCGGAAGCCA GCTCTCCATA
    ACTCAGGTGG CCAGCATCAA GCAACTGCGG GAGACCCAGA GCACCCCCCA GAGTGCACCC
    CAAGTGCGAA GGCCCCTGCA CCCAGCCTTG AACCAGCCAG GCAGCCTCCA GCCCCTGTCC
    TTCCAGAACC CTGTCTATCA CCTCAGTAAC CCCATTCCTG CAATGCCAAA GGCCTCTGTG
    GACTCCAGTT TGGAGAACCT AAGCACTGCC AGTTCCAGGA GCCAAAGTAA CAGTGAAGAC
    TTCAAACTCA GCGGACCCAG CAACAGCAGC ATGGAGGACT TCACCAAACG GAGCACGCAG
    AGTGAGGACT TCTCCAGGCG CCACACCATG CCCGACAGAC ACATACCTCT CGCCCTGCCG
    CGGCAGAACA GCACGGGGCA GACCCAGCTC CGGAAGATGG ACCCGGCGGG GCTGGGCGCC
    CGAGCCAAAG CCCCGCCGTC CCTGCCGCAC AGCGCCTCCC TGCGGAGCAC CGGGAGCATG
    TCGGTGGCAT CAGCGGCTCT GGTGGCCGAG CCCCTGCAGA ACGGGAGCCG GTCCCGACAG
    CAGTCCTCTT CCTCCAGAGA GAGCCCGGTC CCCAAAGTGA GAGCCATCCA GAGGCAGCAG
    ACCCAGCAGG TTCAGTCACC TGTGGACTCC GCCACAATGT CCCCAGTCGA GAGGACAGCA
    GCCTGGGTCC TGAACAATGG GCAGTATGAA GAGGACGTGG AAGAGACTGA GCAAAATCAG
    GACGAAGCCA AGCATGCTGA GAAGTACGAA CAGGAAATCA CCAAACTGAA GGAGCGCCTG
    CGCGTGTCCA GCCGGCGACT CGAGGAGTAT GAGCGCCGGT TGCTAGTGCA GGAGCAGCAG
    ATGCAGAAGC TGCTGCTGGA GTACAAGGCC CGGCTGGAGG ACAGCGAGGA GCGGCTGCGC
    AGGCAGCAGG AGGAGAAGGA CAGCCAGATG AAGAGCATCA TCAGCAGGCT AATGGCTGTG
    GAAGAGGAAC TGAAGAAGGA TCATGCTGAG ATGCAGGCAG TTATTGATGC AAAGCAAAAA
    ATAATCGATG CACAGGTCAT AAATGGAGAT GAGATTATGC AAACAGGAAA AACGGATCGT
    GTCGCTGGAT TCGGCCAACA CCAGACTGAT GAGTGCGCTG ACCCAGGTGA AGGAACGGTA
    CAGCATGCAG GTCCGAAATG GCATCTCCCC CACCAACCCC ACCAAGCTTT CCATCACGGA
    GAATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa08813
    Clone ID Related Accession (Same CDS sequence) XM_005217242.3 , XM_612667.8 , XM_005217242.4
    Accession Version XM_005217242.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3429bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ras GTPase-activating protein nGAP isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005217242.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCAGACCC CAGAGGTGCC AGCAGAAAGG TCCCCACGCA GACGGAGTAT CTCAGGGACC 
    ACTACATCTG AGAAACCCAA CTCTATGGAC ACTGCGAATA CCTCACCCTT CAAAGTACCA
    GGGTTCTTCA GCAAGCGCCT AAAAGGCTCC ATCAAGAGGA CCAAAAGCCA GTCAAAGCTT
    GACCGAAACA CGAGCTTTCG GCTTCCCTCT CTTCGCAATA CCGATGACAG GTCTCGTGGG
    CTGCCTAAAC TAAAAGAGTC CCGTTCTCAT GAATCTTTGC TGAGCCCATG CAGTGCCGTG
    GAATGTCTGG ATCTCGGTAG AGGGGAGCCT GTGTCGGTAA AGCCACTCCA TAGCAGCATC
    CTTGGACAAG ACTTCTGCTT TGAGGTTACC TACTTAAGTG GAAGTAAATG CTTCAGTTGT
    AACTCTGCTT CAGAGAGAGA TAAGTGGATG GAAAACCTGC GCAGGACAGT TCAACCAAAT
    AAGGACAATT GTAGACGAGC TGAAAATGTT CTCCGTTTAT GGATTATTGA AGCCAAGGAC
    CTTGCCCCTA AAAAGAAATA TTTCTGTGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT
    ACAACTAGCA AGACCAAAGC AGACAATATT TTCTGGGGTG AACATTTTGA GTTCTACAGC
    CTTCCACCTC TTCATAGCAT CACAGTTCAC ATTTATAAAG ATGTGGAAAA AAAGAAGAAA
    AAAGACAAGA ATAATTATGT AGGGCTGGTC AACATCCCCA CTGCCAGTGT GACTGGTCGC
    CAGTTTGTAG AAAAGTGGTA CCCAGTGAGC ACACCTACAC CCAACAAAGG AAAGACAGGA
    GGACCTTCTA TTCGGATTAA GTCGCGTTTC CAAACCATCA CCATTCTGCC TATGGAGCAA
    TACAAAGAGT TTGCAGAATT TGTCACCAGC AACTATACCA TGTTATGTTC TGTTCTTGAA
    CCAGTAATCA GTGTGAGAAA TAAAGAAGAG CTAGCCTGTG CCTTAGTGCA CATTCTTCAA
    AGCACTGGAA GGGCCAAGGA TTTTCTGACG GACTTGGTGA TGTCTGAGGT GGATCGTTGT
    GGAGAACATG ATGTCTTGAT CTTCAGAGAG AACACCATTG CCACCAAATC CATTGAGGAA
    TACCTCAAGT TGGTGGGACA ACAGTATCTT CACGATGCAC TGGGGGAGTT CATCAAAGCG
    TTGTATGAGT CAGATGAAAA CTGTGAAGTG GATCCCAGCA AATGTTCATC CAGTGAACTT
    CTGGACCATC AGAGCAACCT GAAAATGTGC TGTGAGCTGG CTTTCTGCAA GATCATCAAC
    TCTTACTGTG TTTTTCCTCG TGAGCTGAAA GAAGTGTTTG CATCCTGGAA GCAGCAGTGC
    CTGAACCGCG GCAAGCAGGA CATCAGCGAG AGGCTCATCA GCGCCTCGCT GTTTCTCCGT
    TTTCTGTGCC CGGCCATCAT GTCTCCCAGT CTCTTCAACC TCATGCAGGA GTATCCTGAT
    GACCGCACAT CTCGGACTCT GACTCTCATT GCCAAGGTCA TCCAGAACCT GGCCAACTTT
    GCCAAATTTG GTAACAAGGA GGAATACATG GCATTCATGA ATGATTTTTT AGAACATGAA
    TGGGGTGGAA TGAAGCGCTT TCTTTTGGAG ATCTCTAATC CAGACACCAT CTCAAACACC
    CCAGGCTTTG ATGGTTACAT TGATCTGGGC CGAGAGCTTT CAGTTTTGCA TTCCTTACTA
    TGGGAAGTAG TTTCCCAACT TGATAAGGGT GAAAATTCCT TCCTACAGGC AACCGTGGCA
    AAATTGGGAC CTCTCCCTCG TGTTCTTGCT GATATTACCA AATCTCTGAC TAATCCTACA
    CCAATACAAC AGCAACTGAG ACGCTTCACT GAGCATAACT CCAGTCCAAA TGTCAGTGGA
    AGCCTCTCCT CTGGGCTGCA GAAAATATTT GAAGACCCTA CTGACAGTGA TTTGCACAAA
    CTAAAATCCC CAAGCCAGGA CAACACAGAC AGTTATTTCA GAGGGAAGAC ATTATTGCTG
    GTCCAGCAAG CCTCCTCCCA GAGCATGACT TACTCGGAAA AGGATGAAAA GGAAAGTAGC
    CTCCCCAATG GCCGCAGCAT CTCCCTCATG GACCTTCAGG AGACGCACGC AGCCCAAGCA
    GAGCATCCAT CTGTGATACT CGACGTGCCC ATGCGCTTGA CCGGAAGCCA GCTCTCCATA
    ACTCAGGTGG CCAGCATCAA GCAACTGCGG GAGACCCAGA GCACCCCCCA GAGTGCACCC
    CAAGTGCGAA GGCCCCTGCA CCCAGCCTTG AACCAGCCAG GCAGCCTCCA GCCCCTGTCC
    TTCCAGAACC CTGTCTATCA CCTCAGTAAC CCCATTCCTG CAATGCCAAA GGCCTCTGTG
    GACTCCAGTT TGGAGAACCT AAGCACTGCC AGTTCCAGGA GCCAAAGTAA CAGTGAAGAC
    TTCAAACTCA GCGGACCCAG CAACAGCAGC ATGGAGGACT TCACCAAACG GAGCACGCAG
    AGTGAGGACT TCTCCAGGCG CCACACCATG CCCGACAGAC ACATACCTCT CGCCCTGCCG
    CGGCAGAACA GCACGGGGCA GACCCAGCTC CGGAAGATGG ACCCGGCGGG GCTGGGCGCC
    CGAGCCAAAG CCCCGCCGTC CCTGCCGCAC AGCGCCTCCC TGCGGAGCAC CGGGAGCATG
    TCGGTGGCAT CAGCGGCTCT GGTGGCCGAG CCCCTGCAGA ACGGGAGCCG GTCCCGACAG
    CAGTCCTCTT CCTCCAGAGA GAGCCCGGTC CCCAAAGTGA GAGCCATCCA GAGGCAGCAG
    ACCCAGCAGG TTCAGTCACC TGTGGACTCC GCCACAATGT CCCCAGTCGA GAGGACAGCA
    GCCTGGGTCC TGAACAATGG GCAGTATGAA GAGGACGTGG AAGAGACTGA GCAAAATCAG
    GACGAAGCCA AGCATGCTGA GAAGTACGAA CAGGAAATCA CCAAACTGAA GGAGCGCCTG
    CGCGTGTCCA GCCGGCGACT CGAGGAGTAT GAGCGCCGGT TGCTAGTGCA GGAGCAGCAG
    ATGCAGAAGC TGCTGCTGGA GTACAAGGCC CGGCTGGAGG ACAGCGAGGA GCGGCTGCGC
    AGGCAGCAGG AGGAGAAGGA CAGCCAGATG AAGAGCATCA TCAGCAGGCT AATGGCTGTG
    GAAGAGGAAC TGAAGAAGGA TCATGCTGAG ATGCAGGCAG TTATTGATGC AAAGCAAAAA
    ATAATCGATG CACAGGTCAT AAATGGAGAT GAGATTATGC AAACAGGAAA AACGGATCGT
    GTCGCTGGAT TCGGCCAACA CCAGACTGAT GAGTGCGCTG ACCCAGGTGA AGGAACGGTA
    CAGCATGCAG GTCCGAAATG GCATCTCCCC CACCAACCCC ACCAAGCTTT CCATCACGGA
    GAATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005217299.1
    CDS142..3570
    Translation

    Target ORF information:

    RefSeq Version XM_005217242.4
    Organism Bos taurus(cattle)
    Definition Bos taurus RAS protein activator like 2 (RASAL2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005217242.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCAGACCC CAGAGGTGCC AGCAGAAAGG TCCCCACGCA GACGGAGTAT CTCAGGGACC 
    ACTACATCTG AGAAACCCAA CTCTATGGAC ACTGCGAATA CCTCACCCTT CAAAGTACCA
    GGGTTCTTCA GCAAGCGCCT AAAAGGCTCC ATCAAGAGGA CCAAAAGCCA GTCAAAGCTT
    GACCGAAACA CGAGCTTTCG GCTTCCCTCT CTTCGCAATA CCGATGACAG GTCTCGTGGG
    CTGCCTAAAC TAAAAGAGTC CCGTTCTCAT GAATCTTTGC TGAGCCCATG CAGTGCCGTG
    GAATGTCTGG ATCTCGGTAG AGGGGAGCCT GTGTCGGTAA AGCCACTCCA TAGCAGCATC
    CTTGGACAAG ACTTCTGCTT TGAGGTTACC TACTTAAGTG GAAGTAAATG CTTCAGTTGT
    AACTCTGCTT CAGAGAGAGA TAAGTGGATG GAAAACCTGC GCAGGACAGT TCAACCAAAT
    AAGGACAATT GTAGACGAGC TGAAAATGTT CTCCGTTTAT GGATTATTGA AGCCAAGGAC
    CTTGCCCCTA AAAAGAAATA TTTCTGTGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT
    ACAACTAGCA AGACCAAAGC AGACAATATT TTCTGGGGTG AACATTTTGA GTTCTACAGC
    CTTCCACCTC TTCATAGCAT CACAGTTCAC ATTTATAAAG ATGTGGAAAA AAAGAAGAAA
    AAAGACAAGA ATAATTATGT AGGGCTGGTC AACATCCCCA CTGCCAGTGT GACTGGTCGC
    CAGTTTGTAG AAAAGTGGTA CCCAGTGAGC ACACCTACAC CCAACAAAGG AAAGACAGGA
    GGACCTTCTA TTCGGATTAA GTCGCGTTTC CAAACCATCA CCATTCTGCC TATGGAGCAA
    TACAAAGAGT TTGCAGAATT TGTCACCAGC AACTATACCA TGTTATGTTC TGTTCTTGAA
    CCAGTAATCA GTGTGAGAAA TAAAGAAGAG CTAGCCTGTG CCTTAGTGCA CATTCTTCAA
    AGCACTGGAA GGGCCAAGGA TTTTCTGACG GACTTGGTGA TGTCTGAGGT GGATCGTTGT
    GGAGAACATG ATGTCTTGAT CTTCAGAGAG AACACCATTG CCACCAAATC CATTGAGGAA
    TACCTCAAGT TGGTGGGACA ACAGTATCTT CACGATGCAC TGGGGGAGTT CATCAAAGCG
    TTGTATGAGT CAGATGAAAA CTGTGAAGTG GATCCCAGCA AATGTTCATC CAGTGAACTT
    CTGGACCATC AGAGCAACCT GAAAATGTGC TGTGAGCTGG CTTTCTGCAA GATCATCAAC
    TCTTACTGTG TTTTTCCTCG TGAGCTGAAA GAAGTGTTTG CATCCTGGAA GCAGCAGTGC
    CTGAACCGCG GCAAGCAGGA CATCAGCGAG AGGCTCATCA GCGCCTCGCT GTTTCTCCGT
    TTTCTGTGCC CGGCCATCAT GTCTCCCAGT CTCTTCAACC TCATGCAGGA GTATCCTGAT
    GACCGCACAT CTCGGACTCT GACTCTCATT GCCAAGGTCA TCCAGAACCT GGCCAACTTT
    GCCAAATTTG GTAACAAGGA GGAATACATG GCATTCATGA ATGATTTTTT AGAACATGAA
    TGGGGTGGAA TGAAGCGCTT TCTTTTGGAG ATCTCTAATC CAGACACCAT CTCAAACACC
    CCAGGCTTTG ATGGTTACAT TGATCTGGGC CGAGAGCTTT CAGTTTTGCA TTCCTTACTA
    TGGGAAGTAG TTTCCCAACT TGATAAGGGT GAAAATTCCT TCCTACAGGC AACCGTGGCA
    AAATTGGGAC CTCTCCCTCG TGTTCTTGCT GATATTACCA AATCTCTGAC TAATCCTACA
    CCAATACAAC AGCAACTGAG ACGCTTCACT GAGCATAACT CCAGTCCAAA TGTCAGTGGA
    AGCCTCTCCT CTGGGCTGCA GAAAATATTT GAAGACCCTA CTGACAGTGA TTTGCACAAA
    CTAAAATCCC CAAGCCAGGA CAACACAGAC AGTTATTTCA GAGGGAAGAC ATTATTGCTG
    GTCCAGCAAG CCTCCTCCCA GAGCATGACT TACTCGGAAA AGGATGAAAA GGAAAGTAGC
    CTCCCCAATG GCCGCAGCAT CTCCCTCATG GACCTTCAGG AGACGCACGC AGCCCAAGCA
    GAGCATCCAT CTGTGATACT CGACGTGCCC ATGCGCTTGA CCGGAAGCCA GCTCTCCATA
    ACTCAGGTGG CCAGCATCAA GCAACTGCGG GAGACCCAGA GCACCCCCCA GAGTGCACCC
    CAAGTGCGAA GGCCCCTGCA CCCAGCCTTG AACCAGCCAG GCAGCCTCCA GCCCCTGTCC
    TTCCAGAACC CTGTCTATCA CCTCAGTAAC CCCATTCCTG CAATGCCAAA GGCCTCTGTG
    GACTCCAGTT TGGAGAACCT AAGCACTGCC AGTTCCAGGA GCCAAAGTAA CAGTGAAGAC
    TTCAAACTCA GCGGACCCAG CAACAGCAGC ATGGAGGACT TCACCAAACG GAGCACGCAG
    AGTGAGGACT TCTCCAGGCG CCACACCATG CCCGACAGAC ACATACCTCT CGCCCTGCCG
    CGGCAGAACA GCACGGGGCA GACCCAGCTC CGGAAGATGG ACCCGGCGGG GCTGGGCGCC
    CGAGCCAAAG CCCCGCCGTC CCTGCCGCAC AGCGCCTCCC TGCGGAGCAC CGGGAGCATG
    TCGGTGGCAT CAGCGGCTCT GGTGGCCGAG CCCCTGCAGA ACGGGAGCCG GTCCCGACAG
    CAGTCCTCTT CCTCCAGAGA GAGCCCGGTC CCCAAAGTGA GAGCCATCCA GAGGCAGCAG
    ACCCAGCAGG TTCAGTCACC TGTGGACTCC GCCACAATGT CCCCAGTCGA GAGGACAGCA
    GCCTGGGTCC TGAACAATGG GCAGTATGAA GAGGACGTGG AAGAGACTGA GCAAAATCAG
    GACGAAGCCA AGCATGCTGA GAAGTACGAA CAGGAAATCA CCAAACTGAA GGAGCGCCTG
    CGCGTGTCCA GCCGGCGACT CGAGGAGTAT GAGCGCCGGT TGCTAGTGCA GGAGCAGCAG
    ATGCAGAAGC TGCTGCTGGA GTACAAGGCC CGGCTGGAGG ACAGCGAGGA GCGGCTGCGC
    AGGCAGCAGG AGGAGAAGGA CAGCCAGATG AAGAGCATCA TCAGCAGGCT AATGGCTGTG
    GAAGAGGAAC TGAAGAAGGA TCATGCTGAG ATGCAGGCAG TTATTGATGC AAAGCAAAAA
    ATAATCGATG CACAGGTCAT AAATGGAGAT GAGATTATGC AAACAGGAAA AACGGATCGT
    GTCGCTGGAT TCGGCCAACA CCAGACTGAT GAGTGCGCTG ACCCAGGTGA AGGAACGGTA
    CAGCATGCAG GTCCGAAATG GCATCTCCCC CACCAACCCC ACCAAGCTTT CCATCACGGA
    GAATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27407
    Clone ID Related Accession (Same CDS sequence) XM_005217241.3 , XM_015466922.1
    Accession Version XM_005217241.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3873bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ras GTPase-activating protein nGAP isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104439.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005217241.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCTCT CCCTGTCGTC CGGAGGAGCC GCGGAGGCGC TGTCCTGGCC AGAGATGTTC 
    CCGGGATTGG AGTCCGACTC GCCGCTCCCC CCGGAGGAGC TGGACGCGGT TGTCCCGGTC
    AGTGGGGCCA TGGCCGGTGG CATGTTGGAT CGGATTCTTC TGGAGTCCGT GTGCCAGCAA
    CAGAGCTGGG TCCGCGTGTA CGATGTGAAA GGGCCACCTA CCCACCGTCT GTCTTGTGGC
    CAGTCGCCCT ACACCGACAC AACAACATGG GAGCGGAAGT ACTGCATCCT CACAGACAGC
    CAGCTGGTGT TGCTCAACAA GGAGAAGGAG ATACCTGTGG ACGGAGGACA AGAACAGCAG
    GCGGATTCCA CCAAAGGGCG ATGCCTACGG AGAACTGTCA GTGTTCCTTC CGAGGGTCAG
    TTTCCTGAAT ACCCACCAGA GGGCGCCACT AAACTGGAGG TGCCAGCAGA AAGGTCCCCA
    CGCAGACGGA GTATCTCAGG GACCACTACA TCTGAGAAAC CCAACTCTAT GGACACTGCG
    AATACCTCAC CCTTCAAAGT ACCAGGGTTC TTCAGCAAGC GCCTAAAAGG CTCCATCAAG
    AGGACCAAAA GCCAGTCAAA GCTTGACCGA AACACGAGCT TTCGGCTTCC CTCTCTTCGC
    AATACCGATG ACAGGTCTCG TGGGCTGCCT AAACTAAAAG AGTCCCGTTC TCATGAATCT
    TTGCTGAGCC CATGCAGTGC CGTGGAATGT CTGGATCTCG GTAGAGGGGA GCCTGTGTCG
    GTAAAGCCAC TCCATAGCAG CATCCTTGGA CAAGACTTCT GCTTTGAGGT TACCTACTTA
    AGTGGAAGTA AATGCTTCAG TTGTAACTCT GCTTCAGAGA GAGATAAGTG GATGGAAAAC
    CTGCGCAGGA CAGTTCAACC AAATAAGGAC AATTGTAGAC GAGCTGAAAA TGTTCTCCGT
    TTATGGATTA TTGAAGCCAA GGACCTTGCC CCTAAAAAGA AATATTTCTG TGAACTGTGC
    CTTGATGATA CCCTCTTTGC TCGTACAACT AGCAAGACCA AAGCAGACAA TATTTTCTGG
    GGTGAACATT TTGAGTTCTA CAGCCTTCCA CCTCTTCATA GCATCACAGT TCACATTTAT
    AAAGATGTGG AAAAAAAGAA GAAAAAAGAC AAGAATAATT ATGTAGGGCT GGTCAACATC
    CCCACTGCCA GTGTGACTGG TCGCCAGTTT GTAGAAAAGT GGTACCCAGT GAGCACACCT
    ACACCCAACA AAGGAAAGAC AGGAGGACCT TCTATTCGGA TTAAGTCGCG TTTCCAAACC
    ATCACCATTC TGCCTATGGA GCAATACAAA GAGTTTGCAG AATTTGTCAC CAGCAACTAT
    ACCATGTTAT GTTCTGTTCT TGAACCAGTA ATCAGTGTGA GAAATAAAGA AGAGCTAGCC
    TGTGCCTTAG TGCACATTCT TCAAAGCACT GGAAGGGCCA AGGATTTTCT GACGGACTTG
    GTGATGTCTG AGGTGGATCG TTGTGGAGAA CATGATGTCT TGATCTTCAG AGAGAACACC
    ATTGCCACCA AATCCATTGA GGAATACCTC AAGTTGGTGG GACAACAGTA TCTTCACGAT
    GCACTGGGGG AGTTCATCAA AGCGTTGTAT GAGTCAGATG AAAACTGTGA AGTGGATCCC
    AGCAAATGTT CATCCAGTGA ACTTCTGGAC CATCAGAGCA ACCTGAAAAT GTGCTGTGAG
    CTGGCTTTCT GCAAGATCAT CAACTCTTAC TGTGTTTTTC CTCGTGAGCT GAAAGAAGTG
    TTTGCATCCT GGAAGCAGCA GTGCCTGAAC CGCGGCAAGC AGGACATCAG CGAGAGGCTC
    ATCAGCGCCT CGCTGTTTCT CCGTTTTCTG TGCCCGGCCA TCATGTCTCC CAGTCTCTTC
    AACCTCATGC AGGAGTATCC TGATGACCGC ACATCTCGGA CTCTGACTCT CATTGCCAAG
    GTCATCCAGA ACCTGGCCAA CTTTGCCAAA TTTGGTAACA AGGAGGAATA CATGGCATTC
    ATGAATGATT TTTTAGAACA TGAATGGGGT GGAATGAAGC GCTTTCTTTT GGAGATCTCT
    AATCCAGACA CCATCTCAAA CACCCCAGGC TTTGATGGTT ACATTGATCT GGGCCGAGAG
    CTTTCAGTTT TGCATTCCTT ACTATGGGAA GTAGTTTCCC AACTTGATAA GGGTGAAAAT
    TCCTTCCTAC AGGCAACCGT GGCAAAATTG GGACCTCTCC CTCGTGTTCT TGCTGATATT
    ACCAAATCTC TGACTAATCC TACACCAATA CAACAGCAAC TGAGACGCTT CACTGAGCAT
    AACTCCAGTC CAAATGTCAG TGGAAGCCTC TCCTCTGGGC TGCAGAAAAT ATTTGAAGAC
    CCTACTGACA GTGATTTGCA CAAACTAAAA TCCCCAAGCC AGGACAACAC AGACAGTTAT
    TTCAGAGGGA AGACATTATT GCTGGTCCAG CAAGCCTCCT CCCAGAGCAT GACTTACTCG
    GAAAAGGATG AAAAGGAAAG TAGCCTCCCC AATGGCCGCA GCATCTCCCT CATGGACCTT
    CAGGAGACGC ACGCAGCCCA AGCAGAGCAT CCATCTGTGA TACTCGACGT GCCCATGCGC
    TTGACCGGAA GCCAGCTCTC CATAACTCAG GTGGCCAGCA TCAAGCAACT GCGGGAGACC
    CAGAGCACCC CCCAGAGTGC ACCCCAAGTG CGAAGGCCCC TGCACCCAGC CTTGAACCAG
    CCAGGCAGCC TCCAGCCCCT GTCCTTCCAG AACCCTGTCT ATCACCTCAG TAACCCCATT
    CCTGCAATGC CAAAGGCCTC TGTGGACTCC AGTTTGGAGA ACCTAAGCAC TGCCAGTTCC
    AGGAGCCAAA GTAACAGTGA AGACTTCAAA CTCAGCGGAC CCAGCAACAG CAGCATGGAG
    GACTTCACCA AACGGAGCAC GCAGAGTGAG GACTTCTCCA GGCGCCACAC CATGCCCGAC
    AGACACATAC CTCTCGCCCT GCCGCGGCAG AACAGCACGG GGCAGACCCA GCTCCGGAAG
    ATGGACCCGG CGGGGCTGGG CGCCCGAGCC AAAGCCCCGC CGTCCCTGCC GCACAGCGCC
    TCCCTGCGGA GCACCGGGAG CATGTCGGTG GCATCAGCGG CTCTGGTGGC CGAGCCCCTG
    CAGAACGGGA GCCGGTCCCG ACAGCAGTCC TCTTCCTCCA GAGAGAGCCC GGTCCCCAAA
    GTGAGAGCCA TCCAGAGGCA GCAGACCCAG CAGGTTCAGT CACCTGTGGA CTCCGCCACA
    ATGTCCCCAG TCGAGAGGAC AGCAGCCTGG GTCCTGAACA ATGGGCAGTA TGAAGAGGAC
    GTGGAAGAGA CTGAGCAAAA TCAGGACGAA GCCAAGCATG CTGAGAAGTA CGAACAGGAA
    ATCACCAAAC TGAAGGAGCG CCTGCGCGTG TCCAGCCGGC GACTCGAGGA GTATGAGCGC
    CGGTTGCTAG TGCAGGAGCA GCAGATGCAG AAGCTGCTGC TGGAGTACAA GGCCCGGCTG
    GAGGACAGCG AGGAGCGGCT GCGCAGGCAG CAGGAGGAGA AGGACAGCCA GATGAAGAGC
    ATCATCAGCA GGCTAATGGC TGTGGAAGAG GAACTGAAGA AGGATCATGC TGAGATGCAG
    GCAGTTATTG ATGCAAAGCA AAAAATAATC GATGCACAGG TCATAAATGG AGATGAGATT
    ATGCAAACAG GAAAAACGGA TCGTGTCGCT GGATTCGGCC AACACCAGAC TGATGAGTGC
    GCTGACCCAG GTGAAGGAAC GGTACAGCAT GCAGGTCCGA AATGGCATCT CCCCCACCAA
    CCCCACCAAG CTTTCCATCA CGGAGAATGG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005217298.1
    CDS97..3969
    Translation

    Target ORF information:

    RefSeq Version XM_005217241.3
    Organism Bos taurus(cattle)
    Definition Bos taurus RAS protein activator like 2 (RASAL2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005217241.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCTCT CCCTGTCGTC CGGAGGAGCC GCGGAGGCGC TGTCCTGGCC AGAGATGTTC 
    CCGGGATTGG AGTCCGACTC GCCGCTCCCC CCGGAGGAGC TGGACGCGGT TGTCCCGGTC
    AGTGGGGCCA TGGCCGGTGG CATGTTGGAT CGGATTCTTC TGGAGTCCGT GTGCCAGCAA
    CAGAGCTGGG TCCGCGTGTA CGATGTGAAA GGGCCACCTA CCCACCGTCT GTCTTGTGGC
    CAGTCGCCCT ACACCGACAC AACAACATGG GAGCGGAAGT ACTGCATCCT CACAGACAGC
    CAGCTGGTGT TGCTCAACAA GGAGAAGGAG ATACCTGTGG ACGGAGGACA AGAACAGCAG
    GCGGATTCCA CCAAAGGGCG ATGCCTACGG AGAACTGTCA GTGTTCCTTC CGAGGGTCAG
    TTTCCTGAAT ACCCACCAGA GGGCGCCACT AAACTGGAGG TGCCAGCAGA AAGGTCCCCA
    CGCAGACGGA GTATCTCAGG GACCACTACA TCTGAGAAAC CCAACTCTAT GGACACTGCG
    AATACCTCAC CCTTCAAAGT ACCAGGGTTC TTCAGCAAGC GCCTAAAAGG CTCCATCAAG
    AGGACCAAAA GCCAGTCAAA GCTTGACCGA AACACGAGCT TTCGGCTTCC CTCTCTTCGC
    AATACCGATG ACAGGTCTCG TGGGCTGCCT AAACTAAAAG AGTCCCGTTC TCATGAATCT
    TTGCTGAGCC CATGCAGTGC CGTGGAATGT CTGGATCTCG GTAGAGGGGA GCCTGTGTCG
    GTAAAGCCAC TCCATAGCAG CATCCTTGGA CAAGACTTCT GCTTTGAGGT TACCTACTTA
    AGTGGAAGTA AATGCTTCAG TTGTAACTCT GCTTCAGAGA GAGATAAGTG GATGGAAAAC
    CTGCGCAGGA CAGTTCAACC AAATAAGGAC AATTGTAGAC GAGCTGAAAA TGTTCTCCGT
    TTATGGATTA TTGAAGCCAA GGACCTTGCC CCTAAAAAGA AATATTTCTG TGAACTGTGC
    CTTGATGATA CCCTCTTTGC TCGTACAACT AGCAAGACCA AAGCAGACAA TATTTTCTGG
    GGTGAACATT TTGAGTTCTA CAGCCTTCCA CCTCTTCATA GCATCACAGT TCACATTTAT
    AAAGATGTGG AAAAAAAGAA GAAAAAAGAC AAGAATAATT ATGTAGGGCT GGTCAACATC
    CCCACTGCCA GTGTGACTGG TCGCCAGTTT GTAGAAAAGT GGTACCCAGT GAGCACACCT
    ACACCCAACA AAGGAAAGAC AGGAGGACCT TCTATTCGGA TTAAGTCGCG TTTCCAAACC
    ATCACCATTC TGCCTATGGA GCAATACAAA GAGTTTGCAG AATTTGTCAC CAGCAACTAT
    ACCATGTTAT GTTCTGTTCT TGAACCAGTA ATCAGTGTGA GAAATAAAGA AGAGCTAGCC
    TGTGCCTTAG TGCACATTCT TCAAAGCACT GGAAGGGCCA AGGATTTTCT GACGGACTTG
    GTGATGTCTG AGGTGGATCG TTGTGGAGAA CATGATGTCT TGATCTTCAG AGAGAACACC
    ATTGCCACCA AATCCATTGA GGAATACCTC AAGTTGGTGG GACAACAGTA TCTTCACGAT
    GCACTGGGGG AGTTCATCAA AGCGTTGTAT GAGTCAGATG AAAACTGTGA AGTGGATCCC
    AGCAAATGTT CATCCAGTGA ACTTCTGGAC CATCAGAGCA ACCTGAAAAT GTGCTGTGAG
    CTGGCTTTCT GCAAGATCAT CAACTCTTAC TGTGTTTTTC CTCGTGAGCT GAAAGAAGTG
    TTTGCATCCT GGAAGCAGCA GTGCCTGAAC CGCGGCAAGC AGGACATCAG CGAGAGGCTC
    ATCAGCGCCT CGCTGTTTCT CCGTTTTCTG TGCCCGGCCA TCATGTCTCC CAGTCTCTTC
    AACCTCATGC AGGAGTATCC TGATGACCGC ACATCTCGGA CTCTGACTCT CATTGCCAAG
    GTCATCCAGA ACCTGGCCAA CTTTGCCAAA TTTGGTAACA AGGAGGAATA CATGGCATTC
    ATGAATGATT TTTTAGAACA TGAATGGGGT GGAATGAAGC GCTTTCTTTT GGAGATCTCT
    AATCCAGACA CCATCTCAAA CACCCCAGGC TTTGATGGTT ACATTGATCT GGGCCGAGAG
    CTTTCAGTTT TGCATTCCTT ACTATGGGAA GTAGTTTCCC AACTTGATAA GGGTGAAAAT
    TCCTTCCTAC AGGCAACCGT GGCAAAATTG GGACCTCTCC CTCGTGTTCT TGCTGATATT
    ACCAAATCTC TGACTAATCC TACACCAATA CAACAGCAAC TGAGACGCTT CACTGAGCAT
    AACTCCAGTC CAAATGTCAG TGGAAGCCTC TCCTCTGGGC TGCAGAAAAT ATTTGAAGAC
    CCTACTGACA GTGATTTGCA CAAACTAAAA TCCCCAAGCC AGGACAACAC AGACAGTTAT
    TTCAGAGGGA AGACATTATT GCTGGTCCAG CAAGCCTCCT CCCAGAGCAT GACTTACTCG
    GAAAAGGATG AAAAGGAAAG TAGCCTCCCC AATGGCCGCA GCATCTCCCT CATGGACCTT
    CAGGAGACGC ACGCAGCCCA AGCAGAGCAT CCATCTGTGA TACTCGACGT GCCCATGCGC
    TTGACCGGAA GCCAGCTCTC CATAACTCAG GTGGCCAGCA TCAAGCAACT GCGGGAGACC
    CAGAGCACCC CCCAGAGTGC ACCCCAAGTG CGAAGGCCCC TGCACCCAGC CTTGAACCAG
    CCAGGCAGCC TCCAGCCCCT GTCCTTCCAG AACCCTGTCT ATCACCTCAG TAACCCCATT
    CCTGCAATGC CAAAGGCCTC TGTGGACTCC AGTTTGGAGA ACCTAAGCAC TGCCAGTTCC
    AGGAGCCAAA GTAACAGTGA AGACTTCAAA CTCAGCGGAC CCAGCAACAG CAGCATGGAG
    GACTTCACCA AACGGAGCAC GCAGAGTGAG GACTTCTCCA GGCGCCACAC CATGCCCGAC
    AGACACATAC CTCTCGCCCT GCCGCGGCAG AACAGCACGG GGCAGACCCA GCTCCGGAAG
    ATGGACCCGG CGGGGCTGGG CGCCCGAGCC AAAGCCCCGC CGTCCCTGCC GCACAGCGCC
    TCCCTGCGGA GCACCGGGAG CATGTCGGTG GCATCAGCGG CTCTGGTGGC CGAGCCCCTG
    CAGAACGGGA GCCGGTCCCG ACAGCAGTCC TCTTCCTCCA GAGAGAGCCC GGTCCCCAAA
    GTGAGAGCCA TCCAGAGGCA GCAGACCCAG CAGGTTCAGT CACCTGTGGA CTCCGCCACA
    ATGTCCCCAG TCGAGAGGAC AGCAGCCTGG GTCCTGAACA ATGGGCAGTA TGAAGAGGAC
    GTGGAAGAGA CTGAGCAAAA TCAGGACGAA GCCAAGCATG CTGAGAAGTA CGAACAGGAA
    ATCACCAAAC TGAAGGAGCG CCTGCGCGTG TCCAGCCGGC GACTCGAGGA GTATGAGCGC
    CGGTTGCTAG TGCAGGAGCA GCAGATGCAG AAGCTGCTGC TGGAGTACAA GGCCCGGCTG
    GAGGACAGCG AGGAGCGGCT GCGCAGGCAG CAGGAGGAGA AGGACAGCCA GATGAAGAGC
    ATCATCAGCA GGCTAATGGC TGTGGAAGAG GAACTGAAGA AGGATCATGC TGAGATGCAG
    GCAGTTATTG ATGCAAAGCA AAAAATAATC GATGCACAGG TCATAAATGG AGATGAGATT
    ATGCAAACAG GAAAAACGGA TCGTGTCGCT GGATTCGGCC AACACCAGAC TGATGAGTGC
    GCTGACCCAG GTGAAGGAAC GGTACAGCAT GCAGGTCCGA AATGGCATCT CCCCCACCAA
    CCCCACCAAG CTTTCCATCA CGGAGAATGG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27407
    Clone ID Related Accession (Same CDS sequence) XM_005217241.3 , XM_015466922.1
    Accession Version XM_015466922.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3873bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ras GTPase-activating protein nGAP isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646324.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCTCT CCCTGTCGTC CGGAGGAGCC GCGGAGGCGC TGTCCTGGCC AGAGATGTTC 
    CCGGGATTGG AGTCCGACTC GCCGCTCCCC CCGGAGGAGC TGGACGCGGT TGTCCCGGTC
    AGTGGGGCCA TGGCCGGTGG CATGTTGGAT CGGATTCTTC TGGAGTCCGT GTGCCAGCAA
    CAGAGCTGGG TCCGCGTGTA CGATGTGAAA GGGCCACCTA CCCACCGTCT GTCTTGTGGC
    CAGTCGCCCT ACACCGACAC AACAACATGG GAGCGGAAGT ACTGCATCCT CACAGACAGC
    CAGCTGGTGT TGCTCAACAA GGAGAAGGAG ATACCTGTGG ACGGAGGACA AGAACAGCAG
    GCGGATTCCA CCAAAGGGCG ATGCCTACGG AGAACTGTCA GTGTTCCTTC CGAGGGTCAG
    TTTCCTGAAT ACCCACCAGA GGGCGCCACT AAACTGGAGG TGCCAGCAGA AAGGTCCCCA
    CGCAGACGGA GTATCTCAGG GACCACTACA TCTGAGAAAC CCAACTCTAT GGACACTGCG
    AATACCTCAC CCTTCAAAGT ACCAGGGTTC TTCAGCAAGC GCCTAAAAGG CTCCATCAAG
    AGGACCAAAA GCCAGTCAAA GCTTGACCGA AACACGAGCT TTCGGCTTCC CTCTCTTCGC
    AATACCGATG ACAGGTCTCG TGGGCTGCCT AAACTAAAAG AGTCCCGTTC TCATGAATCT
    TTGCTGAGCC CATGCAGTGC CGTGGAATGT CTGGATCTCG GTAGAGGGGA GCCTGTGTCG
    GTAAAGCCAC TCCATAGCAG CATCCTTGGA CAAGACTTCT GCTTTGAGGT TACCTACTTA
    AGTGGAAGTA AATGCTTCAG TTGTAACTCT GCTTCAGAGA GAGATAAGTG GATGGAAAAC
    CTGCGCAGGA CAGTTCAACC AAATAAGGAC AATTGTAGAC GAGCTGAAAA TGTTCTCCGT
    TTATGGATTA TTGAAGCCAA GGACCTTGCC CCTAAAAAGA AATATTTCTG TGAACTGTGC
    CTTGATGATA CCCTCTTTGC TCGTACAACT AGCAAGACCA AAGCAGACAA TATTTTCTGG
    GGTGAACATT TTGAGTTCTA CAGCCTTCCA CCTCTTCATA GCATCACAGT TCACATTTAT
    AAAGATGTGG AAAAAAAGAA GAAAAAAGAC AAGAATAATT ATGTAGGGCT GGTCAACATC
    CCCACTGCCA GTGTGACTGG TCGCCAGTTT GTAGAAAAGT GGTACCCAGT GAGCACACCT
    ACACCCAACA AAGGAAAGAC AGGAGGACCT TCTATTCGGA TTAAGTCGCG TTTCCAAACC
    ATCACCATTC TGCCTATGGA GCAATACAAA GAGTTTGCAG AATTTGTCAC CAGCAACTAT
    ACCATGTTAT GTTCTGTTCT TGAACCAGTA ATCAGTGTGA GAAATAAAGA AGAGCTAGCC
    TGTGCCTTAG TGCACATTCT TCAAAGCACT GGAAGGGCCA AGGATTTTCT GACGGACTTG
    GTGATGTCTG AGGTGGATCG TTGTGGAGAA CATGATGTCT TGATCTTCAG AGAGAACACC
    ATTGCCACCA AATCCATTGA GGAATACCTC AAGTTGGTGG GACAACAGTA TCTTCACGAT
    GCACTGGGGG AGTTCATCAA AGCGTTGTAT GAGTCAGATG AAAACTGTGA AGTGGATCCC
    AGCAAATGTT CATCCAGTGA ACTTCTGGAC CATCAGAGCA ACCTGAAAAT GTGCTGTGAG
    CTGGCTTTCT GCAAGATCAT CAACTCTTAC TGTGTTTTTC CTCGTGAGCT GAAAGAAGTG
    TTTGCATCCT GGAAGCAGCA GTGCCTGAAC CGCGGCAAGC AGGACATCAG CGAGAGGCTC
    ATCAGCGCCT CGCTGTTTCT CCGTTTTCTG TGCCCGGCCA TCATGTCTCC CAGTCTCTTC
    AACCTCATGC AGGAGTATCC TGATGACCGC ACATCTCGGA CTCTGACTCT CATTGCCAAG
    GTCATCCAGA ACCTGGCCAA CTTTGCCAAA TTTGGTAACA AGGAGGAATA CATGGCATTC
    ATGAATGATT TTTTAGAACA TGAATGGGGT GGAATGAAGC GCTTTCTTTT GGAGATCTCT
    AATCCAGACA CCATCTCAAA CACCCCAGGC TTTGATGGTT ACATTGATCT GGGCCGAGAG
    CTTTCAGTTT TGCATTCCTT ACTATGGGAA GTAGTTTCCC AACTTGATAA GGGTGAAAAT
    TCCTTCCTAC AGGCAACCGT GGCAAAATTG GGACCTCTCC CTCGTGTTCT TGCTGATATT
    ACCAAATCTC TGACTAATCC TACACCAATA CAACAGCAAC TGAGACGCTT CACTGAGCAT
    AACTCCAGTC CAAATGTCAG TGGAAGCCTC TCCTCTGGGC TGCAGAAAAT ATTTGAAGAC
    CCTACTGACA GTGATTTGCA CAAACTAAAA TCCCCAAGCC AGGACAACAC AGACAGTTAT
    TTCAGAGGGA AGACATTATT GCTGGTCCAG CAAGCCTCCT CCCAGAGCAT GACTTACTCG
    GAAAAGGATG AAAAGGAAAG TAGCCTCCCC AATGGCCGCA GCATCTCCCT CATGGACCTT
    CAGGAGACGC ACGCAGCCCA AGCAGAGCAT CCATCTGTGA TACTCGACGT GCCCATGCGC
    TTGACCGGAA GCCAGCTCTC CATAACTCAG GTGGCCAGCA TCAAGCAACT GCGGGAGACC
    CAGAGCACCC CCCAGAGTGC ACCCCAAGTG CGAAGGCCCC TGCACCCAGC CTTGAACCAG
    CCAGGCAGCC TCCAGCCCCT GTCCTTCCAG AACCCTGTCT ATCACCTCAG TAACCCCATT
    CCTGCAATGC CAAAGGCCTC TGTGGACTCC AGTTTGGAGA ACCTAAGCAC TGCCAGTTCC
    AGGAGCCAAA GTAACAGTGA AGACTTCAAA CTCAGCGGAC CCAGCAACAG CAGCATGGAG
    GACTTCACCA AACGGAGCAC GCAGAGTGAG GACTTCTCCA GGCGCCACAC CATGCCCGAC
    AGACACATAC CTCTCGCCCT GCCGCGGCAG AACAGCACGG GGCAGACCCA GCTCCGGAAG
    ATGGACCCGG CGGGGCTGGG CGCCCGAGCC AAAGCCCCGC CGTCCCTGCC GCACAGCGCC
    TCCCTGCGGA GCACCGGGAG CATGTCGGTG GCATCAGCGG CTCTGGTGGC CGAGCCCCTG
    CAGAACGGGA GCCGGTCCCG ACAGCAGTCC TCTTCCTCCA GAGAGAGCCC GGTCCCCAAA
    GTGAGAGCCA TCCAGAGGCA GCAGACCCAG CAGGTTCAGT CACCTGTGGA CTCCGCCACA
    ATGTCCCCAG TCGAGAGGAC AGCAGCCTGG GTCCTGAACA ATGGGCAGTA TGAAGAGGAC
    GTGGAAGAGA CTGAGCAAAA TCAGGACGAA GCCAAGCATG CTGAGAAGTA CGAACAGGAA
    ATCACCAAAC TGAAGGAGCG CCTGCGCGTG TCCAGCCGGC GACTCGAGGA GTATGAGCGC
    CGGTTGCTAG TGCAGGAGCA GCAGATGCAG AAGCTGCTGC TGGAGTACAA GGCCCGGCTG
    GAGGACAGCG AGGAGCGGCT GCGCAGGCAG CAGGAGGAGA AGGACAGCCA GATGAAGAGC
    ATCATCAGCA GGCTAATGGC TGTGGAAGAG GAACTGAAGA AGGATCATGC TGAGATGCAG
    GCAGTTATTG ATGCAAAGCA AAAAATAATC GATGCACAGG TCATAAATGG AGATGAGATT
    ATGCAAACAG GAAAAACGGA TCGTGTCGCT GGATTCGGCC AACACCAGAC TGATGAGTGC
    GCTGACCCAG GTGAAGGAAC GGTACAGCAT GCAGGTCCGA AATGGCATCT CCCCCACCAA
    CCCCACCAAG CTTTCCATCA CGGAGAATGG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322408.1
    CDS97..3969
    Translation

    Target ORF information:

    RefSeq Version XM_015466922.1
    Organism Bos taurus(cattle)
    Definition Bos taurus RAS protein activator like 2 (RASAL2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015466922.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCTCT CCCTGTCGTC CGGAGGAGCC GCGGAGGCGC TGTCCTGGCC AGAGATGTTC 
    CCGGGATTGG AGTCCGACTC GCCGCTCCCC CCGGAGGAGC TGGACGCGGT TGTCCCGGTC
    AGTGGGGCCA TGGCCGGTGG CATGTTGGAT CGGATTCTTC TGGAGTCCGT GTGCCAGCAA
    CAGAGCTGGG TCCGCGTGTA CGATGTGAAA GGGCCACCTA CCCACCGTCT GTCTTGTGGC
    CAGTCGCCCT ACACCGACAC AACAACATGG GAGCGGAAGT ACTGCATCCT CACAGACAGC
    CAGCTGGTGT TGCTCAACAA GGAGAAGGAG ATACCTGTGG ACGGAGGACA AGAACAGCAG
    GCGGATTCCA CCAAAGGGCG ATGCCTACGG AGAACTGTCA GTGTTCCTTC CGAGGGTCAG
    TTTCCTGAAT ACCCACCAGA GGGCGCCACT AAACTGGAGG TGCCAGCAGA AAGGTCCCCA
    CGCAGACGGA GTATCTCAGG GACCACTACA TCTGAGAAAC CCAACTCTAT GGACACTGCG
    AATACCTCAC CCTTCAAAGT ACCAGGGTTC TTCAGCAAGC GCCTAAAAGG CTCCATCAAG
    AGGACCAAAA GCCAGTCAAA GCTTGACCGA AACACGAGCT TTCGGCTTCC CTCTCTTCGC
    AATACCGATG ACAGGTCTCG TGGGCTGCCT AAACTAAAAG AGTCCCGTTC TCATGAATCT
    TTGCTGAGCC CATGCAGTGC CGTGGAATGT CTGGATCTCG GTAGAGGGGA GCCTGTGTCG
    GTAAAGCCAC TCCATAGCAG CATCCTTGGA CAAGACTTCT GCTTTGAGGT TACCTACTTA
    AGTGGAAGTA AATGCTTCAG TTGTAACTCT GCTTCAGAGA GAGATAAGTG GATGGAAAAC
    CTGCGCAGGA CAGTTCAACC AAATAAGGAC AATTGTAGAC GAGCTGAAAA TGTTCTCCGT
    TTATGGATTA TTGAAGCCAA GGACCTTGCC CCTAAAAAGA AATATTTCTG TGAACTGTGC
    CTTGATGATA CCCTCTTTGC TCGTACAACT AGCAAGACCA AAGCAGACAA TATTTTCTGG
    GGTGAACATT TTGAGTTCTA CAGCCTTCCA CCTCTTCATA GCATCACAGT TCACATTTAT
    AAAGATGTGG AAAAAAAGAA GAAAAAAGAC AAGAATAATT ATGTAGGGCT GGTCAACATC
    CCCACTGCCA GTGTGACTGG TCGCCAGTTT GTAGAAAAGT GGTACCCAGT GAGCACACCT
    ACACCCAACA AAGGAAAGAC AGGAGGACCT TCTATTCGGA TTAAGTCGCG TTTCCAAACC
    ATCACCATTC TGCCTATGGA GCAATACAAA GAGTTTGCAG AATTTGTCAC CAGCAACTAT
    ACCATGTTAT GTTCTGTTCT TGAACCAGTA ATCAGTGTGA GAAATAAAGA AGAGCTAGCC
    TGTGCCTTAG TGCACATTCT TCAAAGCACT GGAAGGGCCA AGGATTTTCT GACGGACTTG
    GTGATGTCTG AGGTGGATCG TTGTGGAGAA CATGATGTCT TGATCTTCAG AGAGAACACC
    ATTGCCACCA AATCCATTGA GGAATACCTC AAGTTGGTGG GACAACAGTA TCTTCACGAT
    GCACTGGGGG AGTTCATCAA AGCGTTGTAT GAGTCAGATG AAAACTGTGA AGTGGATCCC
    AGCAAATGTT CATCCAGTGA ACTTCTGGAC CATCAGAGCA ACCTGAAAAT GTGCTGTGAG
    CTGGCTTTCT GCAAGATCAT CAACTCTTAC TGTGTTTTTC CTCGTGAGCT GAAAGAAGTG
    TTTGCATCCT GGAAGCAGCA GTGCCTGAAC CGCGGCAAGC AGGACATCAG CGAGAGGCTC
    ATCAGCGCCT CGCTGTTTCT CCGTTTTCTG TGCCCGGCCA TCATGTCTCC CAGTCTCTTC
    AACCTCATGC AGGAGTATCC TGATGACCGC ACATCTCGGA CTCTGACTCT CATTGCCAAG
    GTCATCCAGA ACCTGGCCAA CTTTGCCAAA TTTGGTAACA AGGAGGAATA CATGGCATTC
    ATGAATGATT TTTTAGAACA TGAATGGGGT GGAATGAAGC GCTTTCTTTT GGAGATCTCT
    AATCCAGACA CCATCTCAAA CACCCCAGGC TTTGATGGTT ACATTGATCT GGGCCGAGAG
    CTTTCAGTTT TGCATTCCTT ACTATGGGAA GTAGTTTCCC AACTTGATAA GGGTGAAAAT
    TCCTTCCTAC AGGCAACCGT GGCAAAATTG GGACCTCTCC CTCGTGTTCT TGCTGATATT
    ACCAAATCTC TGACTAATCC TACACCAATA CAACAGCAAC TGAGACGCTT CACTGAGCAT
    AACTCCAGTC CAAATGTCAG TGGAAGCCTC TCCTCTGGGC TGCAGAAAAT ATTTGAAGAC
    CCTACTGACA GTGATTTGCA CAAACTAAAA TCCCCAAGCC AGGACAACAC AGACAGTTAT
    TTCAGAGGGA AGACATTATT GCTGGTCCAG CAAGCCTCCT CCCAGAGCAT GACTTACTCG
    GAAAAGGATG AAAAGGAAAG TAGCCTCCCC AATGGCCGCA GCATCTCCCT CATGGACCTT
    CAGGAGACGC ACGCAGCCCA AGCAGAGCAT CCATCTGTGA TACTCGACGT GCCCATGCGC
    TTGACCGGAA GCCAGCTCTC CATAACTCAG GTGGCCAGCA TCAAGCAACT GCGGGAGACC
    CAGAGCACCC CCCAGAGTGC ACCCCAAGTG CGAAGGCCCC TGCACCCAGC CTTGAACCAG
    CCAGGCAGCC TCCAGCCCCT GTCCTTCCAG AACCCTGTCT ATCACCTCAG TAACCCCATT
    CCTGCAATGC CAAAGGCCTC TGTGGACTCC AGTTTGGAGA ACCTAAGCAC TGCCAGTTCC
    AGGAGCCAAA GTAACAGTGA AGACTTCAAA CTCAGCGGAC CCAGCAACAG CAGCATGGAG
    GACTTCACCA AACGGAGCAC GCAGAGTGAG GACTTCTCCA GGCGCCACAC CATGCCCGAC
    AGACACATAC CTCTCGCCCT GCCGCGGCAG AACAGCACGG GGCAGACCCA GCTCCGGAAG
    ATGGACCCGG CGGGGCTGGG CGCCCGAGCC AAAGCCCCGC CGTCCCTGCC GCACAGCGCC
    TCCCTGCGGA GCACCGGGAG CATGTCGGTG GCATCAGCGG CTCTGGTGGC CGAGCCCCTG
    CAGAACGGGA GCCGGTCCCG ACAGCAGTCC TCTTCCTCCA GAGAGAGCCC GGTCCCCAAA
    GTGAGAGCCA TCCAGAGGCA GCAGACCCAG CAGGTTCAGT CACCTGTGGA CTCCGCCACA
    ATGTCCCCAG TCGAGAGGAC AGCAGCCTGG GTCCTGAACA ATGGGCAGTA TGAAGAGGAC
    GTGGAAGAGA CTGAGCAAAA TCAGGACGAA GCCAAGCATG CTGAGAAGTA CGAACAGGAA
    ATCACCAAAC TGAAGGAGCG CCTGCGCGTG TCCAGCCGGC GACTCGAGGA GTATGAGCGC
    CGGTTGCTAG TGCAGGAGCA GCAGATGCAG AAGCTGCTGC TGGAGTACAA GGCCCGGCTG
    GAGGACAGCG AGGAGCGGCT GCGCAGGCAG CAGGAGGAGA AGGACAGCCA GATGAAGAGC
    ATCATCAGCA GGCTAATGGC TGTGGAAGAG GAACTGAAGA AGGATCATGC TGAGATGCAG
    GCAGTTATTG ATGCAAAGCA AAAAATAATC GATGCACAGG TCATAAATGG AGATGAGATT
    ATGCAAACAG GAAAAACGGA TCGTGTCGCT GGATTCGGCC AACACCAGAC TGATGAGTGC
    GCTGACCCAG GTGAAGGAAC GGTACAGCAT GCAGGTCCGA AATGGCATCT CCCCCACCAA
    CCCCACCAAG CTTTCCATCA CGGAGAATGG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27408
    Clone ID Related Accession (Same CDS sequence) XM_002694157.5 , XM_015466923.1
    Accession Version XM_002694157.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3864bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ras GTPase-activating protein nGAP isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104439.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002694157.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCTCT CCCTGTCGTC CGGAGGAGCC GCGGAGGCGC TGTCCTGGCC AGAGATGTTC 
    CCGGGATTGG AGTCCGACTC GCCGCTCCCC CCGGAGGAGC TGGACGCGGT TGTCCCGGTC
    AGTGGGGCCA TGGCCGGTGG CATGTTGGAT CGGATTCTTC TGGAGTCCGT GTGCCAGCAA
    CAGAGCTGGG TCCGCGTGTA CGATGTGAAA GGGCCACCTA CCCACCGTCT GTCTTGTGGC
    CAGTCGCCCT ACACCGACAC AACAACATGG GAGCGGAAGT ACTGCATCCT CACAGACAGC
    CAGCTGGTGT TGCTCAACAA GGAGAAGGAG ATACCTGTGG ACGGAGGACA AGAACAGCAG
    GCGGATTCCA CCAAAGGGCG ATGCCTACGG AGAACTGTCA GTGTTCCTTC CGAGGGTCAG
    TTTCCTGAAT ACCCACCAGA GGGCGCCACT AAACTGGAGG TGCCAGCAGA AAGGTCCCCA
    CGCAGACGGA GTATCTCAGG GACCACTACA TCTGAGAAAC CCAACTCTAT GGACACTGCG
    AATACCTCAC CCTTCAAAGT ACCAGGGTTC TTCAGCAAGC GCCTAAAAGG CTCCATCAAG
    AGGACCAAAA GCCAGTCAAA GCTTGACCGA AACACGAGCT TTCGGCTTCC CTCTCTTCGC
    AATACCGATG ACAGGTCTCG TGGGCTGCCT AAACTAAAAG AGTCCCGTTC TCATGAATCT
    TTGCTGAGCC CATGCAGTGC CGTGGAATGT CTGGATCTCG GTAGAGGGGA GCCTGTGTCG
    GTAAAGCCAC TCCATAGCAG CATCCTTGGA CAAGACTTCT GCTTTGAGGT TACCTACTTA
    AGTGGAAGTA AATGCTTCAG TTGTAACTCT GCTTCAGAGA GAGATAAGTG GATGGAAAAC
    CTGCGCAGGA CAGTTCAACC AAATAAGGAC AATTGTAGAC GAGCTGAAAA TGTTCTCCGT
    TTATGGATTA TTGAAGCCAA GGACCTTGCC CCTAAAAAGA AATATTTCTG TGAACTGTGC
    CTTGATGATA CCCTCTTTGC TCGTACAACT AGCAAGACCA AAGCAGACAA TATTTTCTGG
    GGTGAACATT TTGAGTTCTA CAGCCTTCCA CCTCTTCATA GCATCACAGT TCACATTTAT
    AAAGATGTGG AAAAAAAGAA GAAAAAAGAC AAGAATAATT ATGTAGGGCT GGTCAACATC
    CCCACTGCCA GTGTGACTGG TCGCCAGTTT GTAGAAAAGT GGTACCCAGT GAGCACACCT
    ACACCCAACA AAGGAAAGAC AGGAGGACCT TCTATTCGGA TTAAGTCGCG TTTCCAAACC
    ATCACCATTC TGCCTATGGA GCAATACAAA GAGTTTGCAG AATTTGTCAC CAGCAACTAT
    ACCATGTTAT GTTCTGTTCT TGAACCAGTA ATCAGTGTGA GAAATAAAGA AGAGCTAGCC
    TGTGCCTTAG TGCACATTCT TCAAAGCACT GGAAGGGCCA AGGATTTTCT GACGGACTTG
    GTGATGTCTG AGGTGGATCG TTGTGGAGAA CATGATGTCT TGATCTTCAG AGAGAACACC
    ATTGCCACCA AATCCATTGA GGAATACCTC AAGTTGGTGG GACAACAGTA TCTTCACGAT
    GCACTGGGGG AGTTCATCAA AGCGTTGTAT GAGTCAGATG AAAACTGTGA AGTGGATCCC
    AGCAAATGTT CATCCAGTGA ACTTCTGGAC CATCAGAGCA ACCTGAAAAT GTGCTGTGAG
    CTGGCTTTCT GCAAGATCAT CAACTCTTAC TGTGTTTTTC CTCGTGAGCT GAAAGAAGTG
    TTTGCATCCT GGAAGCAGCA GTGCCTGAAC CGCGGCAAGC AGGACATCAG CGAGAGGCTC
    ATCAGCGCCT CGCTGTTTCT CCGTTTTCTG TGCCCGGCCA TCATGTCTCC CAGTCTCTTC
    AACCTCATGC AGGAGTATCC TGATGACCGC ACATCTCGGA CTCTGACTCT CATTGCCAAG
    GTCATCCAGA ACCTGGCCAA CTTTGCCAAA TTTGGTAACA AGGAGGAATA CATGGCATTC
    ATGAATGATT TTTTAGAACA TGAATGGGGT GGAATGAAGC GCTTTCTTTT GGAGATCTCT
    AATCCAGACA CCATCTCAAA CACCCCAGGC TTTGATGGTT ACATTGATCT GGGCCGAGAG
    CTTTCAGTTT TGCATTCCTT ACTATGGGAA GTAGTTTCCC AACTTGATAA GGGTGAAAAT
    TCCTTCCTAC AGGCAACCGT GGCAAAATTG GGACCTCTCC CTCGTGTTCT TGCTGATATT
    ACCAAATCTC TGACTAATCC TACACCAATA CAACAGCAAC TGAGACGCTT CACTGAGCAT
    AACTCCAGTC CAAATGTCAG TGGAAGCCTC TCCTCTGGGC TGCAGAAAAT ATTTGAAGAC
    CCTACTGACA GTGATTTGCA CAAACTAAAA TCCCCAAGCC AGGACAACAC AGACAGTTAT
    TTCAGAGGGA AGACATTATT GCTGGTCCAG CAAGCCTCCT CCCAGAGCAT GACTTACTCG
    GAAAAGGATG AAAAGGAAAG TAGCCTCCCC AATGGCCGCA GCATCTCCCT CATGGACCTT
    CAGGAGACGC ACGCAGCCCA AGCAGAGCAT CCATCTGTGA TACTCGACGT GCCCATGCGC
    TTGACCGGAA GCCAGCTCTC CATAACTCAG GTGGCCAGCA TCAAGCAACT GCGGGAGACC
    CAGAGCACCC CCCAGAGTGC ACCCCAAGTG CGAAGGCCCC TGCACCCAGC CTTGAACCAG
    CCAGGCAGCC TCCAGCCCCT GTCCTTCCAG AACCCTGTCT ATCACCTCAG TAACCCCATT
    CCTGCAATGC CAAAGGCCTC TGTGGACTCC AGTTTGGAGA ACCTAAGCAC TGCCAGTTCC
    AGGAGCCAAA GTAACAGTGA AGACTTCAAA CTCAGCGGAC CCAGCAACAG CAGCATGGAG
    GACTTCACCA AACGGAGCAC GCAGAGTGAG GACTTCTCCA GGCGCCACAC CATGCCCGAC
    AGACACATAC CTCTCGCCCT GCCGCGGCAG AACAGCACGG GGCAGACCCA GCTCCGGAAG
    ATGGACCCGG CGGGGCTGGG CGCCCGAGCC AAAGCCCCGC CGTCCCTGCC GCACAGCGCC
    TCCCTGCGGA GCACCGGGAG CATGTCGGTG GCATCAGCGG CTCTGGTGGC CGAGCCCCTG
    CAGAACGGGA GCCGGTCCCG ACAGCAGTCC TCTTCCTCCA GAGAGAGCCC GGTCCCCAAA
    GTGAGAGCCA TCCAGAGGCA GCAGACCCAG CAGGTTCAGT CACCTGTGGA CTCCGCCACA
    ATGTCCCCAG TCGAGAGGAC AGCAGCCTGG GTCCTGAACA ATGGGCAGTA TGAAGAGGAC
    GTGGAAGAGA CTGAGCAAAA TCAGGACGAA GCCAAGCATG CTGAGAAGTA CGAACAGGAA
    ATCACCAAAC TGAAGGAGCG CCTGCGCGTG TCCAGCCGGC GACTCGAGGA GTATGAGCGC
    CGGTTGCTAG TGCAGGAGCA GCAGATGCAG AAGCTGCTGC TGGAGTACAA GGCCCGGCTG
    GAGGACAGCG AGGAGCGGCT GCGCAGGCAG CAGGAGGAGA AGGACAGCCA GATGAAGAGC
    ATCATCAGCA GGCTAATGGC TGTGGAAGAG GAACTGAAGA AGGATCATGC TGAGATGCAG
    GCAGTTATTG ATGCAAAGCA AAAAATAATC GATGCACAGG AAAAACGGAT CGTGTCGCTG
    GATTCGGCCA ACACCAGACT GATGAGTGCG CTGACCCAGG TGAAGGAACG GTACAGCATG
    CAGGTCCGAA ATGGCATCTC CCCCACCAAC CCCACCAAGC TTTCCATCAC GGAGAATGGT
    GAATTCAAAA ACAGCAGCTG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002694203.1
    CDS96..3959
    Translation

    Target ORF information:

    RefSeq Version XM_002694157.5
    Organism Bos taurus(cattle)
    Definition Bos taurus RAS protein activator like 2 (RASAL2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002694157.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCTCT CCCTGTCGTC CGGAGGAGCC GCGGAGGCGC TGTCCTGGCC AGAGATGTTC 
    CCGGGATTGG AGTCCGACTC GCCGCTCCCC CCGGAGGAGC TGGACGCGGT TGTCCCGGTC
    AGTGGGGCCA TGGCCGGTGG CATGTTGGAT CGGATTCTTC TGGAGTCCGT GTGCCAGCAA
    CAGAGCTGGG TCCGCGTGTA CGATGTGAAA GGGCCACCTA CCCACCGTCT GTCTTGTGGC
    CAGTCGCCCT ACACCGACAC AACAACATGG GAGCGGAAGT ACTGCATCCT CACAGACAGC
    CAGCTGGTGT TGCTCAACAA GGAGAAGGAG ATACCTGTGG ACGGAGGACA AGAACAGCAG
    GCGGATTCCA CCAAAGGGCG ATGCCTACGG AGAACTGTCA GTGTTCCTTC CGAGGGTCAG
    TTTCCTGAAT ACCCACCAGA GGGCGCCACT AAACTGGAGG TGCCAGCAGA AAGGTCCCCA
    CGCAGACGGA GTATCTCAGG GACCACTACA TCTGAGAAAC CCAACTCTAT GGACACTGCG
    AATACCTCAC CCTTCAAAGT ACCAGGGTTC TTCAGCAAGC GCCTAAAAGG CTCCATCAAG
    AGGACCAAAA GCCAGTCAAA GCTTGACCGA AACACGAGCT TTCGGCTTCC CTCTCTTCGC
    AATACCGATG ACAGGTCTCG TGGGCTGCCT AAACTAAAAG AGTCCCGTTC TCATGAATCT
    TTGCTGAGCC CATGCAGTGC CGTGGAATGT CTGGATCTCG GTAGAGGGGA GCCTGTGTCG
    GTAAAGCCAC TCCATAGCAG CATCCTTGGA CAAGACTTCT GCTTTGAGGT TACCTACTTA
    AGTGGAAGTA AATGCTTCAG TTGTAACTCT GCTTCAGAGA GAGATAAGTG GATGGAAAAC
    CTGCGCAGGA CAGTTCAACC AAATAAGGAC AATTGTAGAC GAGCTGAAAA TGTTCTCCGT
    TTATGGATTA TTGAAGCCAA GGACCTTGCC CCTAAAAAGA AATATTTCTG TGAACTGTGC
    CTTGATGATA CCCTCTTTGC TCGTACAACT AGCAAGACCA AAGCAGACAA TATTTTCTGG
    GGTGAACATT TTGAGTTCTA CAGCCTTCCA CCTCTTCATA GCATCACAGT TCACATTTAT
    AAAGATGTGG AAAAAAAGAA GAAAAAAGAC AAGAATAATT ATGTAGGGCT GGTCAACATC
    CCCACTGCCA GTGTGACTGG TCGCCAGTTT GTAGAAAAGT GGTACCCAGT GAGCACACCT
    ACACCCAACA AAGGAAAGAC AGGAGGACCT TCTATTCGGA TTAAGTCGCG TTTCCAAACC
    ATCACCATTC TGCCTATGGA GCAATACAAA GAGTTTGCAG AATTTGTCAC CAGCAACTAT
    ACCATGTTAT GTTCTGTTCT TGAACCAGTA ATCAGTGTGA GAAATAAAGA AGAGCTAGCC
    TGTGCCTTAG TGCACATTCT TCAAAGCACT GGAAGGGCCA AGGATTTTCT GACGGACTTG
    GTGATGTCTG AGGTGGATCG TTGTGGAGAA CATGATGTCT TGATCTTCAG AGAGAACACC
    ATTGCCACCA AATCCATTGA GGAATACCTC AAGTTGGTGG GACAACAGTA TCTTCACGAT
    GCACTGGGGG AGTTCATCAA AGCGTTGTAT GAGTCAGATG AAAACTGTGA AGTGGATCCC
    AGCAAATGTT CATCCAGTGA ACTTCTGGAC CATCAGAGCA ACCTGAAAAT GTGCTGTGAG
    CTGGCTTTCT GCAAGATCAT CAACTCTTAC TGTGTTTTTC CTCGTGAGCT GAAAGAAGTG
    TTTGCATCCT GGAAGCAGCA GTGCCTGAAC CGCGGCAAGC AGGACATCAG CGAGAGGCTC
    ATCAGCGCCT CGCTGTTTCT CCGTTTTCTG TGCCCGGCCA TCATGTCTCC CAGTCTCTTC
    AACCTCATGC AGGAGTATCC TGATGACCGC ACATCTCGGA CTCTGACTCT CATTGCCAAG
    GTCATCCAGA ACCTGGCCAA CTTTGCCAAA TTTGGTAACA AGGAGGAATA CATGGCATTC
    ATGAATGATT TTTTAGAACA TGAATGGGGT GGAATGAAGC GCTTTCTTTT GGAGATCTCT
    AATCCAGACA CCATCTCAAA CACCCCAGGC TTTGATGGTT ACATTGATCT GGGCCGAGAG
    CTTTCAGTTT TGCATTCCTT ACTATGGGAA GTAGTTTCCC AACTTGATAA GGGTGAAAAT
    TCCTTCCTAC AGGCAACCGT GGCAAAATTG GGACCTCTCC CTCGTGTTCT TGCTGATATT
    ACCAAATCTC TGACTAATCC TACACCAATA CAACAGCAAC TGAGACGCTT CACTGAGCAT
    AACTCCAGTC CAAATGTCAG TGGAAGCCTC TCCTCTGGGC TGCAGAAAAT ATTTGAAGAC
    CCTACTGACA GTGATTTGCA CAAACTAAAA TCCCCAAGCC AGGACAACAC AGACAGTTAT
    TTCAGAGGGA AGACATTATT GCTGGTCCAG CAAGCCTCCT CCCAGAGCAT GACTTACTCG
    GAAAAGGATG AAAAGGAAAG TAGCCTCCCC AATGGCCGCA GCATCTCCCT CATGGACCTT
    CAGGAGACGC ACGCAGCCCA AGCAGAGCAT CCATCTGTGA TACTCGACGT GCCCATGCGC
    TTGACCGGAA GCCAGCTCTC CATAACTCAG GTGGCCAGCA TCAAGCAACT GCGGGAGACC
    CAGAGCACCC CCCAGAGTGC ACCCCAAGTG CGAAGGCCCC TGCACCCAGC CTTGAACCAG
    CCAGGCAGCC TCCAGCCCCT GTCCTTCCAG AACCCTGTCT ATCACCTCAG TAACCCCATT
    CCTGCAATGC CAAAGGCCTC TGTGGACTCC AGTTTGGAGA ACCTAAGCAC TGCCAGTTCC
    AGGAGCCAAA GTAACAGTGA AGACTTCAAA CTCAGCGGAC CCAGCAACAG CAGCATGGAG
    GACTTCACCA AACGGAGCAC GCAGAGTGAG GACTTCTCCA GGCGCCACAC CATGCCCGAC
    AGACACATAC CTCTCGCCCT GCCGCGGCAG AACAGCACGG GGCAGACCCA GCTCCGGAAG
    ATGGACCCGG CGGGGCTGGG CGCCCGAGCC AAAGCCCCGC CGTCCCTGCC GCACAGCGCC
    TCCCTGCGGA GCACCGGGAG CATGTCGGTG GCATCAGCGG CTCTGGTGGC CGAGCCCCTG
    CAGAACGGGA GCCGGTCCCG ACAGCAGTCC TCTTCCTCCA GAGAGAGCCC GGTCCCCAAA
    GTGAGAGCCA TCCAGAGGCA GCAGACCCAG CAGGTTCAGT CACCTGTGGA CTCCGCCACA
    ATGTCCCCAG TCGAGAGGAC AGCAGCCTGG GTCCTGAACA ATGGGCAGTA TGAAGAGGAC
    GTGGAAGAGA CTGAGCAAAA TCAGGACGAA GCCAAGCATG CTGAGAAGTA CGAACAGGAA
    ATCACCAAAC TGAAGGAGCG CCTGCGCGTG TCCAGCCGGC GACTCGAGGA GTATGAGCGC
    CGGTTGCTAG TGCAGGAGCA GCAGATGCAG AAGCTGCTGC TGGAGTACAA GGCCCGGCTG
    GAGGACAGCG AGGAGCGGCT GCGCAGGCAG CAGGAGGAGA AGGACAGCCA GATGAAGAGC
    ATCATCAGCA GGCTAATGGC TGTGGAAGAG GAACTGAAGA AGGATCATGC TGAGATGCAG
    GCAGTTATTG ATGCAAAGCA AAAAATAATC GATGCACAGG AAAAACGGAT CGTGTCGCTG
    GATTCGGCCA ACACCAGACT GATGAGTGCG CTGACCCAGG TGAAGGAACG GTACAGCATG
    CAGGTCCGAA ATGGCATCTC CCCCACCAAC CCCACCAAGC TTTCCATCAC GGAGAATGGT
    GAATTCAAAA ACAGCAGCTG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27408
    Clone ID Related Accession (Same CDS sequence) XM_002694157.5 , XM_015466923.1
    Accession Version XM_015466923.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3864bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ras GTPase-activating protein nGAP isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646324.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCTCT CCCTGTCGTC CGGAGGAGCC GCGGAGGCGC TGTCCTGGCC AGAGATGTTC 
    CCGGGATTGG AGTCCGACTC GCCGCTCCCC CCGGAGGAGC TGGACGCGGT TGTCCCGGTC
    AGTGGGGCCA TGGCCGGTGG CATGTTGGAT CGGATTCTTC TGGAGTCCGT GTGCCAGCAA
    CAGAGCTGGG TCCGCGTGTA CGATGTGAAA GGGCCACCTA CCCACCGTCT GTCTTGTGGC
    CAGTCGCCCT ACACCGACAC AACAACATGG GAGCGGAAGT ACTGCATCCT CACAGACAGC
    CAGCTGGTGT TGCTCAACAA GGAGAAGGAG ATACCTGTGG ACGGAGGACA AGAACAGCAG
    GCGGATTCCA CCAAAGGGCG ATGCCTACGG AGAACTGTCA GTGTTCCTTC CGAGGGTCAG
    TTTCCTGAAT ACCCACCAGA GGGCGCCACT AAACTGGAGG TGCCAGCAGA AAGGTCCCCA
    CGCAGACGGA GTATCTCAGG GACCACTACA TCTGAGAAAC CCAACTCTAT GGACACTGCG
    AATACCTCAC CCTTCAAAGT ACCAGGGTTC TTCAGCAAGC GCCTAAAAGG CTCCATCAAG
    AGGACCAAAA GCCAGTCAAA GCTTGACCGA AACACGAGCT TTCGGCTTCC CTCTCTTCGC
    AATACCGATG ACAGGTCTCG TGGGCTGCCT AAACTAAAAG AGTCCCGTTC TCATGAATCT
    TTGCTGAGCC CATGCAGTGC CGTGGAATGT CTGGATCTCG GTAGAGGGGA GCCTGTGTCG
    GTAAAGCCAC TCCATAGCAG CATCCTTGGA CAAGACTTCT GCTTTGAGGT TACCTACTTA
    AGTGGAAGTA AATGCTTCAG TTGTAACTCT GCTTCAGAGA GAGATAAGTG GATGGAAAAC
    CTGCGCAGGA CAGTTCAACC AAATAAGGAC AATTGTAGAC GAGCTGAAAA TGTTCTCCGT
    TTATGGATTA TTGAAGCCAA GGACCTTGCC CCTAAAAAGA AATATTTCTG TGAACTGTGC
    CTTGATGATA CCCTCTTTGC TCGTACAACT AGCAAGACCA AAGCAGACAA TATTTTCTGG
    GGTGAACATT TTGAGTTCTA CAGCCTTCCA CCTCTTCATA GCATCACAGT TCACATTTAT
    AAAGATGTGG AAAAAAAGAA GAAAAAAGAC AAGAATAATT ATGTAGGGCT GGTCAACATC
    CCCACTGCCA GTGTGACTGG TCGCCAGTTT GTAGAAAAGT GGTACCCAGT GAGCACACCT
    ACACCCAACA AAGGAAAGAC AGGAGGACCT TCTATTCGGA TTAAGTCGCG TTTCCAAACC
    ATCACCATTC TGCCTATGGA GCAATACAAA GAGTTTGCAG AATTTGTCAC CAGCAACTAT
    ACCATGTTAT GTTCTGTTCT TGAACCAGTA ATCAGTGTGA GAAATAAAGA AGAGCTAGCC
    TGTGCCTTAG TGCACATTCT TCAAAGCACT GGAAGGGCCA AGGATTTTCT GACGGACTTG
    GTGATGTCTG AGGTGGATCG TTGTGGAGAA CATGATGTCT TGATCTTCAG AGAGAACACC
    ATTGCCACCA AATCCATTGA GGAATACCTC AAGTTGGTGG GACAACAGTA TCTTCACGAT
    GCACTGGGGG AGTTCATCAA AGCGTTGTAT GAGTCAGATG AAAACTGTGA AGTGGATCCC
    AGCAAATGTT CATCCAGTGA ACTTCTGGAC CATCAGAGCA ACCTGAAAAT GTGCTGTGAG
    CTGGCTTTCT GCAAGATCAT CAACTCTTAC TGTGTTTTTC CTCGTGAGCT GAAAGAAGTG
    TTTGCATCCT GGAAGCAGCA GTGCCTGAAC CGCGGCAAGC AGGACATCAG CGAGAGGCTC
    ATCAGCGCCT CGCTGTTTCT CCGTTTTCTG TGCCCGGCCA TCATGTCTCC CAGTCTCTTC
    AACCTCATGC AGGAGTATCC TGATGACCGC ACATCTCGGA CTCTGACTCT CATTGCCAAG
    GTCATCCAGA ACCTGGCCAA CTTTGCCAAA TTTGGTAACA AGGAGGAATA CATGGCATTC
    ATGAATGATT TTTTAGAACA TGAATGGGGT GGAATGAAGC GCTTTCTTTT GGAGATCTCT
    AATCCAGACA CCATCTCAAA CACCCCAGGC TTTGATGGTT ACATTGATCT GGGCCGAGAG
    CTTTCAGTTT TGCATTCCTT ACTATGGGAA GTAGTTTCCC AACTTGATAA GGGTGAAAAT
    TCCTTCCTAC AGGCAACCGT GGCAAAATTG GGACCTCTCC CTCGTGTTCT TGCTGATATT
    ACCAAATCTC TGACTAATCC TACACCAATA CAACAGCAAC TGAGACGCTT CACTGAGCAT
    AACTCCAGTC CAAATGTCAG TGGAAGCCTC TCCTCTGGGC TGCAGAAAAT ATTTGAAGAC
    CCTACTGACA GTGATTTGCA CAAACTAAAA TCCCCAAGCC AGGACAACAC AGACAGTTAT
    TTCAGAGGGA AGACATTATT GCTGGTCCAG CAAGCCTCCT CCCAGAGCAT GACTTACTCG
    GAAAAGGATG AAAAGGAAAG TAGCCTCCCC AATGGCCGCA GCATCTCCCT CATGGACCTT
    CAGGAGACGC ACGCAGCCCA AGCAGAGCAT CCATCTGTGA TACTCGACGT GCCCATGCGC
    TTGACCGGAA GCCAGCTCTC CATAACTCAG GTGGCCAGCA TCAAGCAACT GCGGGAGACC
    CAGAGCACCC CCCAGAGTGC ACCCCAAGTG CGAAGGCCCC TGCACCCAGC CTTGAACCAG
    CCAGGCAGCC TCCAGCCCCT GTCCTTCCAG AACCCTGTCT ATCACCTCAG TAACCCCATT
    CCTGCAATGC CAAAGGCCTC TGTGGACTCC AGTTTGGAGA ACCTAAGCAC TGCCAGTTCC
    AGGAGCCAAA GTAACAGTGA AGACTTCAAA CTCAGCGGAC CCAGCAACAG CAGCATGGAG
    GACTTCACCA AACGGAGCAC GCAGAGTGAG GACTTCTCCA GGCGCCACAC CATGCCCGAC
    AGACACATAC CTCTCGCCCT GCCGCGGCAG AACAGCACGG GGCAGACCCA GCTCCGGAAG
    ATGGACCCGG CGGGGCTGGG CGCCCGAGCC AAAGCCCCGC CGTCCCTGCC GCACAGCGCC
    TCCCTGCGGA GCACCGGGAG CATGTCGGTG GCATCAGCGG CTCTGGTGGC CGAGCCCCTG
    CAGAACGGGA GCCGGTCCCG ACAGCAGTCC TCTTCCTCCA GAGAGAGCCC GGTCCCCAAA
    GTGAGAGCCA TCCAGAGGCA GCAGACCCAG CAGGTTCAGT CACCTGTGGA CTCCGCCACA
    ATGTCCCCAG TCGAGAGGAC AGCAGCCTGG GTCCTGAACA ATGGGCAGTA TGAAGAGGAC
    GTGGAAGAGA CTGAGCAAAA TCAGGACGAA GCCAAGCATG CTGAGAAGTA CGAACAGGAA
    ATCACCAAAC TGAAGGAGCG CCTGCGCGTG TCCAGCCGGC GACTCGAGGA GTATGAGCGC
    CGGTTGCTAG TGCAGGAGCA GCAGATGCAG AAGCTGCTGC TGGAGTACAA GGCCCGGCTG
    GAGGACAGCG AGGAGCGGCT GCGCAGGCAG CAGGAGGAGA AGGACAGCCA GATGAAGAGC
    ATCATCAGCA GGCTAATGGC TGTGGAAGAG GAACTGAAGA AGGATCATGC TGAGATGCAG
    GCAGTTATTG ATGCAAAGCA AAAAATAATC GATGCACAGG AAAAACGGAT CGTGTCGCTG
    GATTCGGCCA ACACCAGACT GATGAGTGCG CTGACCCAGG TGAAGGAACG GTACAGCATG
    CAGGTCCGAA ATGGCATCTC CCCCACCAAC CCCACCAAGC TTTCCATCAC GGAGAATGGT
    GAATTCAAAA ACAGCAGCTG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322409.1
    CDS97..3960
    Translation

    Target ORF information:

    RefSeq Version XM_015466923.1
    Organism Bos taurus(cattle)
    Definition Bos taurus RAS protein activator like 2 (RASAL2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015466923.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCTCT CCCTGTCGTC CGGAGGAGCC GCGGAGGCGC TGTCCTGGCC AGAGATGTTC 
    CCGGGATTGG AGTCCGACTC GCCGCTCCCC CCGGAGGAGC TGGACGCGGT TGTCCCGGTC
    AGTGGGGCCA TGGCCGGTGG CATGTTGGAT CGGATTCTTC TGGAGTCCGT GTGCCAGCAA
    CAGAGCTGGG TCCGCGTGTA CGATGTGAAA GGGCCACCTA CCCACCGTCT GTCTTGTGGC
    CAGTCGCCCT ACACCGACAC AACAACATGG GAGCGGAAGT ACTGCATCCT CACAGACAGC
    CAGCTGGTGT TGCTCAACAA GGAGAAGGAG ATACCTGTGG ACGGAGGACA AGAACAGCAG
    GCGGATTCCA CCAAAGGGCG ATGCCTACGG AGAACTGTCA GTGTTCCTTC CGAGGGTCAG
    TTTCCTGAAT ACCCACCAGA GGGCGCCACT AAACTGGAGG TGCCAGCAGA AAGGTCCCCA
    CGCAGACGGA GTATCTCAGG GACCACTACA TCTGAGAAAC CCAACTCTAT GGACACTGCG
    AATACCTCAC CCTTCAAAGT ACCAGGGTTC TTCAGCAAGC GCCTAAAAGG CTCCATCAAG
    AGGACCAAAA GCCAGTCAAA GCTTGACCGA AACACGAGCT TTCGGCTTCC CTCTCTTCGC
    AATACCGATG ACAGGTCTCG TGGGCTGCCT AAACTAAAAG AGTCCCGTTC TCATGAATCT
    TTGCTGAGCC CATGCAGTGC CGTGGAATGT CTGGATCTCG GTAGAGGGGA GCCTGTGTCG
    GTAAAGCCAC TCCATAGCAG CATCCTTGGA CAAGACTTCT GCTTTGAGGT TACCTACTTA
    AGTGGAAGTA AATGCTTCAG TTGTAACTCT GCTTCAGAGA GAGATAAGTG GATGGAAAAC
    CTGCGCAGGA CAGTTCAACC AAATAAGGAC AATTGTAGAC GAGCTGAAAA TGTTCTCCGT
    TTATGGATTA TTGAAGCCAA GGACCTTGCC CCTAAAAAGA AATATTTCTG TGAACTGTGC
    CTTGATGATA CCCTCTTTGC TCGTACAACT AGCAAGACCA AAGCAGACAA TATTTTCTGG
    GGTGAACATT TTGAGTTCTA CAGCCTTCCA CCTCTTCATA GCATCACAGT TCACATTTAT
    AAAGATGTGG AAAAAAAGAA GAAAAAAGAC AAGAATAATT ATGTAGGGCT GGTCAACATC
    CCCACTGCCA GTGTGACTGG TCGCCAGTTT GTAGAAAAGT GGTACCCAGT GAGCACACCT
    ACACCCAACA AAGGAAAGAC AGGAGGACCT TCTATTCGGA TTAAGTCGCG TTTCCAAACC
    ATCACCATTC TGCCTATGGA GCAATACAAA GAGTTTGCAG AATTTGTCAC CAGCAACTAT
    ACCATGTTAT GTTCTGTTCT TGAACCAGTA ATCAGTGTGA GAAATAAAGA AGAGCTAGCC
    TGTGCCTTAG TGCACATTCT TCAAAGCACT GGAAGGGCCA AGGATTTTCT GACGGACTTG
    GTGATGTCTG AGGTGGATCG TTGTGGAGAA CATGATGTCT TGATCTTCAG AGAGAACACC
    ATTGCCACCA AATCCATTGA GGAATACCTC AAGTTGGTGG GACAACAGTA TCTTCACGAT
    GCACTGGGGG AGTTCATCAA AGCGTTGTAT GAGTCAGATG AAAACTGTGA AGTGGATCCC
    AGCAAATGTT CATCCAGTGA ACTTCTGGAC CATCAGAGCA ACCTGAAAAT GTGCTGTGAG
    CTGGCTTTCT GCAAGATCAT CAACTCTTAC TGTGTTTTTC CTCGTGAGCT GAAAGAAGTG
    TTTGCATCCT GGAAGCAGCA GTGCCTGAAC CGCGGCAAGC AGGACATCAG CGAGAGGCTC
    ATCAGCGCCT CGCTGTTTCT CCGTTTTCTG TGCCCGGCCA TCATGTCTCC CAGTCTCTTC
    AACCTCATGC AGGAGTATCC TGATGACCGC ACATCTCGGA CTCTGACTCT CATTGCCAAG
    GTCATCCAGA ACCTGGCCAA CTTTGCCAAA TTTGGTAACA AGGAGGAATA CATGGCATTC
    ATGAATGATT TTTTAGAACA TGAATGGGGT GGAATGAAGC GCTTTCTTTT GGAGATCTCT
    AATCCAGACA CCATCTCAAA CACCCCAGGC TTTGATGGTT ACATTGATCT GGGCCGAGAG
    CTTTCAGTTT TGCATTCCTT ACTATGGGAA GTAGTTTCCC AACTTGATAA GGGTGAAAAT
    TCCTTCCTAC AGGCAACCGT GGCAAAATTG GGACCTCTCC CTCGTGTTCT TGCTGATATT
    ACCAAATCTC TGACTAATCC TACACCAATA CAACAGCAAC TGAGACGCTT CACTGAGCAT
    AACTCCAGTC CAAATGTCAG TGGAAGCCTC TCCTCTGGGC TGCAGAAAAT ATTTGAAGAC
    CCTACTGACA GTGATTTGCA CAAACTAAAA TCCCCAAGCC AGGACAACAC AGACAGTTAT
    TTCAGAGGGA AGACATTATT GCTGGTCCAG CAAGCCTCCT CCCAGAGCAT GACTTACTCG
    GAAAAGGATG AAAAGGAAAG TAGCCTCCCC AATGGCCGCA GCATCTCCCT CATGGACCTT
    CAGGAGACGC ACGCAGCCCA AGCAGAGCAT CCATCTGTGA TACTCGACGT GCCCATGCGC
    TTGACCGGAA GCCAGCTCTC CATAACTCAG GTGGCCAGCA TCAAGCAACT GCGGGAGACC
    CAGAGCACCC CCCAGAGTGC ACCCCAAGTG CGAAGGCCCC TGCACCCAGC CTTGAACCAG
    CCAGGCAGCC TCCAGCCCCT GTCCTTCCAG AACCCTGTCT ATCACCTCAG TAACCCCATT
    CCTGCAATGC CAAAGGCCTC TGTGGACTCC AGTTTGGAGA ACCTAAGCAC TGCCAGTTCC
    AGGAGCCAAA GTAACAGTGA AGACTTCAAA CTCAGCGGAC CCAGCAACAG CAGCATGGAG
    GACTTCACCA AACGGAGCAC GCAGAGTGAG GACTTCTCCA GGCGCCACAC CATGCCCGAC
    AGACACATAC CTCTCGCCCT GCCGCGGCAG AACAGCACGG GGCAGACCCA GCTCCGGAAG
    ATGGACCCGG CGGGGCTGGG CGCCCGAGCC AAAGCCCCGC CGTCCCTGCC GCACAGCGCC
    TCCCTGCGGA GCACCGGGAG CATGTCGGTG GCATCAGCGG CTCTGGTGGC CGAGCCCCTG
    CAGAACGGGA GCCGGTCCCG ACAGCAGTCC TCTTCCTCCA GAGAGAGCCC GGTCCCCAAA
    GTGAGAGCCA TCCAGAGGCA GCAGACCCAG CAGGTTCAGT CACCTGTGGA CTCCGCCACA
    ATGTCCCCAG TCGAGAGGAC AGCAGCCTGG GTCCTGAACA ATGGGCAGTA TGAAGAGGAC
    GTGGAAGAGA CTGAGCAAAA TCAGGACGAA GCCAAGCATG CTGAGAAGTA CGAACAGGAA
    ATCACCAAAC TGAAGGAGCG CCTGCGCGTG TCCAGCCGGC GACTCGAGGA GTATGAGCGC
    CGGTTGCTAG TGCAGGAGCA GCAGATGCAG AAGCTGCTGC TGGAGTACAA GGCCCGGCTG
    GAGGACAGCG AGGAGCGGCT GCGCAGGCAG CAGGAGGAGA AGGACAGCCA GATGAAGAGC
    ATCATCAGCA GGCTAATGGC TGTGGAAGAG GAACTGAAGA AGGATCATGC TGAGATGCAG
    GCAGTTATTG ATGCAAAGCA AAAAATAATC GATGCACAGG AAAAACGGAT CGTGTCGCTG
    GATTCGGCCA ACACCAGACT GATGAGTGCG CTGACCCAGG TGAAGGAACG GTACAGCATG
    CAGGTCCGAA ATGGCATCTC CCCCACCAAC CCCACCAAGC TTTCCATCAC GGAGAATGGT
    GAATTCAAAA ACAGCAGCTG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27409
    Clone ID Related Accession (Same CDS sequence) XM_015466924.1 , XM_003587091.4
    Accession Version XM_015466924.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3843bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ras GTPase-activating protein nGAP isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646324.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCTCT CCCTGTCGTC CGGAGGAGCC GCGGAGGCGC TGTCCTGGCC AGAGATGTTC 
    CCGGGATTGG AGTCCGACTC GCCGCTCCCC CCGGAGGAGC TGGACGCGGT TGTCCCGGTC
    AGTGGGGCCA TGGCCGGTGG CATGTTGGAT CGGATTCTTC TGGAGTCCGT GTGCCAGCAA
    CAGAGCTGGG TCCGCGTGTA CGATGTGAAA GGGCCACCTA CCCACCGTCT GTCTTGTGGC
    CAGTCGCCCT ACACCGACAC AACAACATGG GAGCGGAAGT ACTGCATCCT CACAGACAGC
    CAGCTGGTGT TGCTCAACAA GGAGAAGGAG ATACCTGTGG ACGGAGGACA AGAACAGCAG
    GCGGATTCCA CCAAAGGGCG ATGCCTACGG AGAACTGTCA GTGTTCCTTC CGAGGGTCAG
    TTTCCTGAAT ACCCACCAGA GGGCGCCACT AAACTGGAGG TGCCAGCAGA AAGGTCCCCA
    CGCAGACGGA GTATCTCAGG GACCACTACA TCTGAGAAAC CCAACTCTAT GGACACTGCG
    AATACCTCAC CCTTCAAAGT ACCAGGGTTC TTCAGCAAGC GCCTAAAAGG CTCCATCAAG
    AGGACCAAAA GCCAGTCAAA GCTTGACCGA AACACGAGCT TTCGGCTTCC CTCTCTTCGC
    AATACCGATG ACAGGTCTCG TGGGCTGCCT AAACTAAAAG AGTCCCGTTC TCATGAATCT
    TTGCTGAGCC CATGCAGTGC CGTGGAATGT CTGGATCTCG GTAGAGGGGA GCCTGTGTCG
    GTAAAGCCAC TCCATAGCAG CATCCTTGGA CAAGACTTCT GCTTTGAGGT TACCTACTTA
    AGTGGAAGTA AATGCTTCAG TTGTAACTCT GCTTCAGAGA GAGATAAGTG GATGGAAAAC
    CTGCGCAGGA CAGTTCAACC AAATAAGGAC AATTGTAGAC GAGCTGAAAA TGTTCTCCGT
    TTATGGATTA TTGAAGCCAA GGACCTTGCC CCTAAAAAGA AATATTTCTG TGAACTGTGC
    CTTGATGATA CCCTCTTTGC TCGTACAACT AGCAAGACCA AAGCAGACAA TATTTTCTGG
    GGTGAACATT TTGAGTTCTA CAGCCTTCCA CCTCTTCATA GCATCACAGT TCACATTTAT
    AAAGATGTGG AAAAAAAGAA GAAAAAAGAC AAGAATAATT ATGTAGGGCT GGTCAACATC
    CCCACTGCCA GTGTGACTGG TCGCCAGTTT GTAGAAAAGT GGTACCCAGT GAGCACACCT
    ACACCCAACA AAGGAAAGAC AGGAGGACCT TCTATTCGGA TTAAGTCGCG TTTCCAAACC
    ATCACCATTC TGCCTATGGA GCAATACAAA GAGTTTGCAG AATTTGTCAC CAGCAACTAT
    ACCATGTTAT GTTCTGTTCT TGAACCAGTA ATCAGTGTGA GAAATAAAGA AGAGCTAGCC
    TGTGCCTTAG TGCACATTCT TCAAAGCACT GGAAGGGCCA AGGATTTTCT GACGGACTTG
    GTGATGTCTG AGGTGGATCG TTGTGGAGAA CATGATGTCT TGATCTTCAG AGAGAACACC
    ATTGCCACCA AATCCATTGA GGAATACCTC AAGTTGGTGG GACAACAGTA TCTTCACGAT
    GCACTGGGGG AGTTCATCAA AGCGTTGTAT GAGTCAGATG AAAACTGTGA AGTGGATCCC
    AGCAAATGTT CATCCAGTGA ACTTCTGGAC CATCAGAGCA ACCTGAAAAT GTGCTGTGAG
    CTGGCTTTCT GCAAGATCAT CAACTCTTAC TGTGTTTTTC CTCGTGAGCT GAAAGAAGTG
    TTTGCATCCT GGAAGCAGCA GTGCCTGAAC CGCGGCAAGC AGGACATCAG CGAGAGGCTC
    ATCAGCGCCT CGCTGTTTCT CCGTTTTCTG TGCCCGGCCA TCATGTCTCC CAGTCTCTTC
    AACCTCATGC AGGAGTATCC TGATGACCGC ACATCTCGGA CTCTGACTCT CATTGCCAAG
    GTCATCCAGA ACCTGGCCAA CTTTGCCAAA TTTGGTAACA AGGAGGAATA CATGGCATTC
    ATGAATGATT TTTTAGAACA TGAATGGGGT GGAATGAAGC GCTTTCTTTT GGAGATCTCT
    AATCCAGACA CCATCTCAAA CACCCCAGGC TTTGATGGTT ACATTGATCT GGGCCGAGAG
    CTTTCAGTTT TGCATTCCTT ACTATGGGAA GTAGTTTCCC AACTTGATAA GGCAACCGTG
    GCAAAATTGG GACCTCTCCC TCGTGTTCTT GCTGATATTA CCAAATCTCT GACTAATCCT
    ACACCAATAC AACAGCAACT GAGACGCTTC ACTGAGCATA ACTCCAGTCC AAATGTCAGT
    GGAAGCCTCT CCTCTGGGCT GCAGAAAATA TTTGAAGACC CTACTGACAG TGATTTGCAC
    AAACTAAAAT CCCCAAGCCA GGACAACACA GACAGTTATT TCAGAGGGAA GACATTATTG
    CTGGTCCAGC AAGCCTCCTC CCAGAGCATG ACTTACTCGG AAAAGGATGA AAAGGAAAGT
    AGCCTCCCCA ATGGCCGCAG CATCTCCCTC ATGGACCTTC AGGAGACGCA CGCAGCCCAA
    GCAGAGCATC CATCTGTGAT ACTCGACGTG CCCATGCGCT TGACCGGAAG CCAGCTCTCC
    ATAACTCAGG TGGCCAGCAT CAAGCAACTG CGGGAGACCC AGAGCACCCC CCAGAGTGCA
    CCCCAAGTGC GAAGGCCCCT GCACCCAGCC TTGAACCAGC CAGGCAGCCT CCAGCCCCTG
    TCCTTCCAGA ACCCTGTCTA TCACCTCAGT AACCCCATTC CTGCAATGCC AAAGGCCTCT
    GTGGACTCCA GTTTGGAGAA CCTAAGCACT GCCAGTTCCA GGAGCCAAAG TAACAGTGAA
    GACTTCAAAC TCAGCGGACC CAGCAACAGC AGCATGGAGG ACTTCACCAA ACGGAGCACG
    CAGAGTGAGG ACTTCTCCAG GCGCCACACC ATGCCCGACA GACACATACC TCTCGCCCTG
    CCGCGGCAGA ACAGCACGGG GCAGACCCAG CTCCGGAAGA TGGACCCGGC GGGGCTGGGC
    GCCCGAGCCA AAGCCCCGCC GTCCCTGCCG CACAGCGCCT CCCTGCGGAG CACCGGGAGC
    ATGTCGGTGG CATCAGCGGC TCTGGTGGCC GAGCCCCTGC AGAACGGGAG CCGGTCCCGA
    CAGCAGTCCT CTTCCTCCAG AGAGAGCCCG GTCCCCAAAG TGAGAGCCAT CCAGAGGCAG
    CAGACCCAGC AGGTTCAGTC ACCTGTGGAC TCCGCCACAA TGTCCCCAGT CGAGAGGACA
    GCAGCCTGGG TCCTGAACAA TGGGCAGTAT GAAGAGGACG TGGAAGAGAC TGAGCAAAAT
    CAGGACGAAG CCAAGCATGC TGAGAAGTAC GAACAGGAAA TCACCAAACT GAAGGAGCGC
    CTGCGCGTGT CCAGCCGGCG ACTCGAGGAG TATGAGCGCC GGTTGCTAGT GCAGGAGCAG
    CAGATGCAGA AGCTGCTGCT GGAGTACAAG GCCCGGCTGG AGGACAGCGA GGAGCGGCTG
    CGCAGGCAGC AGGAGGAGAA GGACAGCCAG ATGAAGAGCA TCATCAGCAG GCTAATGGCT
    GTGGAAGAGG AACTGAAGAA GGATCATGCT GAGATGCAGG CAGTTATTGA TGCAAAGCAA
    AAAATAATCG ATGCACAGGA AAAACGGATC GTGTCGCTGG ATTCGGCCAA CACCAGACTG
    ATGAGTGCGC TGACCCAGGT GAAGGAACGG TACAGCATGC AGGTCCGAAA TGGCATCTCC
    CCCACCAACC CCACCAAGCT TTCCATCACG GAGAATGGTG AATTCAAAAA CAGCAGCTGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322410.1
    CDS97..3939
    Translation

    Target ORF information:

    RefSeq Version XM_015466924.1
    Organism Bos taurus(cattle)
    Definition Bos taurus RAS protein activator like 2 (RASAL2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015466924.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCTCT CCCTGTCGTC CGGAGGAGCC GCGGAGGCGC TGTCCTGGCC AGAGATGTTC 
    CCGGGATTGG AGTCCGACTC GCCGCTCCCC CCGGAGGAGC TGGACGCGGT TGTCCCGGTC
    AGTGGGGCCA TGGCCGGTGG CATGTTGGAT CGGATTCTTC TGGAGTCCGT GTGCCAGCAA
    CAGAGCTGGG TCCGCGTGTA CGATGTGAAA GGGCCACCTA CCCACCGTCT GTCTTGTGGC
    CAGTCGCCCT ACACCGACAC AACAACATGG GAGCGGAAGT ACTGCATCCT CACAGACAGC
    CAGCTGGTGT TGCTCAACAA GGAGAAGGAG ATACCTGTGG ACGGAGGACA AGAACAGCAG
    GCGGATTCCA CCAAAGGGCG ATGCCTACGG AGAACTGTCA GTGTTCCTTC CGAGGGTCAG
    TTTCCTGAAT ACCCACCAGA GGGCGCCACT AAACTGGAGG TGCCAGCAGA AAGGTCCCCA
    CGCAGACGGA GTATCTCAGG GACCACTACA TCTGAGAAAC CCAACTCTAT GGACACTGCG
    AATACCTCAC CCTTCAAAGT ACCAGGGTTC TTCAGCAAGC GCCTAAAAGG CTCCATCAAG
    AGGACCAAAA GCCAGTCAAA GCTTGACCGA AACACGAGCT TTCGGCTTCC CTCTCTTCGC
    AATACCGATG ACAGGTCTCG TGGGCTGCCT AAACTAAAAG AGTCCCGTTC TCATGAATCT
    TTGCTGAGCC CATGCAGTGC CGTGGAATGT CTGGATCTCG GTAGAGGGGA GCCTGTGTCG
    GTAAAGCCAC TCCATAGCAG CATCCTTGGA CAAGACTTCT GCTTTGAGGT TACCTACTTA
    AGTGGAAGTA AATGCTTCAG TTGTAACTCT GCTTCAGAGA GAGATAAGTG GATGGAAAAC
    CTGCGCAGGA CAGTTCAACC AAATAAGGAC AATTGTAGAC GAGCTGAAAA TGTTCTCCGT
    TTATGGATTA TTGAAGCCAA GGACCTTGCC CCTAAAAAGA AATATTTCTG TGAACTGTGC
    CTTGATGATA CCCTCTTTGC TCGTACAACT AGCAAGACCA AAGCAGACAA TATTTTCTGG
    GGTGAACATT TTGAGTTCTA CAGCCTTCCA CCTCTTCATA GCATCACAGT TCACATTTAT
    AAAGATGTGG AAAAAAAGAA GAAAAAAGAC AAGAATAATT ATGTAGGGCT GGTCAACATC
    CCCACTGCCA GTGTGACTGG TCGCCAGTTT GTAGAAAAGT GGTACCCAGT GAGCACACCT
    ACACCCAACA AAGGAAAGAC AGGAGGACCT TCTATTCGGA TTAAGTCGCG TTTCCAAACC
    ATCACCATTC TGCCTATGGA GCAATACAAA GAGTTTGCAG AATTTGTCAC CAGCAACTAT
    ACCATGTTAT GTTCTGTTCT TGAACCAGTA ATCAGTGTGA GAAATAAAGA AGAGCTAGCC
    TGTGCCTTAG TGCACATTCT TCAAAGCACT GGAAGGGCCA AGGATTTTCT GACGGACTTG
    GTGATGTCTG AGGTGGATCG TTGTGGAGAA CATGATGTCT TGATCTTCAG AGAGAACACC
    ATTGCCACCA AATCCATTGA GGAATACCTC AAGTTGGTGG GACAACAGTA TCTTCACGAT
    GCACTGGGGG AGTTCATCAA AGCGTTGTAT GAGTCAGATG AAAACTGTGA AGTGGATCCC
    AGCAAATGTT CATCCAGTGA ACTTCTGGAC CATCAGAGCA ACCTGAAAAT GTGCTGTGAG
    CTGGCTTTCT GCAAGATCAT CAACTCTTAC TGTGTTTTTC CTCGTGAGCT GAAAGAAGTG
    TTTGCATCCT GGAAGCAGCA GTGCCTGAAC CGCGGCAAGC AGGACATCAG CGAGAGGCTC
    ATCAGCGCCT CGCTGTTTCT CCGTTTTCTG TGCCCGGCCA TCATGTCTCC CAGTCTCTTC
    AACCTCATGC AGGAGTATCC TGATGACCGC ACATCTCGGA CTCTGACTCT CATTGCCAAG
    GTCATCCAGA ACCTGGCCAA CTTTGCCAAA TTTGGTAACA AGGAGGAATA CATGGCATTC
    ATGAATGATT TTTTAGAACA TGAATGGGGT GGAATGAAGC GCTTTCTTTT GGAGATCTCT
    AATCCAGACA CCATCTCAAA CACCCCAGGC TTTGATGGTT ACATTGATCT GGGCCGAGAG
    CTTTCAGTTT TGCATTCCTT ACTATGGGAA GTAGTTTCCC AACTTGATAA GGCAACCGTG
    GCAAAATTGG GACCTCTCCC TCGTGTTCTT GCTGATATTA CCAAATCTCT GACTAATCCT
    ACACCAATAC AACAGCAACT GAGACGCTTC ACTGAGCATA ACTCCAGTCC AAATGTCAGT
    GGAAGCCTCT CCTCTGGGCT GCAGAAAATA TTTGAAGACC CTACTGACAG TGATTTGCAC
    AAACTAAAAT CCCCAAGCCA GGACAACACA GACAGTTATT TCAGAGGGAA GACATTATTG
    CTGGTCCAGC AAGCCTCCTC CCAGAGCATG ACTTACTCGG AAAAGGATGA AAAGGAAAGT
    AGCCTCCCCA ATGGCCGCAG CATCTCCCTC ATGGACCTTC AGGAGACGCA CGCAGCCCAA
    GCAGAGCATC CATCTGTGAT ACTCGACGTG CCCATGCGCT TGACCGGAAG CCAGCTCTCC
    ATAACTCAGG TGGCCAGCAT CAAGCAACTG CGGGAGACCC AGAGCACCCC CCAGAGTGCA
    CCCCAAGTGC GAAGGCCCCT GCACCCAGCC TTGAACCAGC CAGGCAGCCT CCAGCCCCTG
    TCCTTCCAGA ACCCTGTCTA TCACCTCAGT AACCCCATTC CTGCAATGCC AAAGGCCTCT
    GTGGACTCCA GTTTGGAGAA CCTAAGCACT GCCAGTTCCA GGAGCCAAAG TAACAGTGAA
    GACTTCAAAC TCAGCGGACC CAGCAACAGC AGCATGGAGG ACTTCACCAA ACGGAGCACG
    CAGAGTGAGG ACTTCTCCAG GCGCCACACC ATGCCCGACA GACACATACC TCTCGCCCTG
    CCGCGGCAGA ACAGCACGGG GCAGACCCAG CTCCGGAAGA TGGACCCGGC GGGGCTGGGC
    GCCCGAGCCA AAGCCCCGCC GTCCCTGCCG CACAGCGCCT CCCTGCGGAG CACCGGGAGC
    ATGTCGGTGG CATCAGCGGC TCTGGTGGCC GAGCCCCTGC AGAACGGGAG CCGGTCCCGA
    CAGCAGTCCT CTTCCTCCAG AGAGAGCCCG GTCCCCAAAG TGAGAGCCAT CCAGAGGCAG
    CAGACCCAGC AGGTTCAGTC ACCTGTGGAC TCCGCCACAA TGTCCCCAGT CGAGAGGACA
    GCAGCCTGGG TCCTGAACAA TGGGCAGTAT GAAGAGGACG TGGAAGAGAC TGAGCAAAAT
    CAGGACGAAG CCAAGCATGC TGAGAAGTAC GAACAGGAAA TCACCAAACT GAAGGAGCGC
    CTGCGCGTGT CCAGCCGGCG ACTCGAGGAG TATGAGCGCC GGTTGCTAGT GCAGGAGCAG
    CAGATGCAGA AGCTGCTGCT GGAGTACAAG GCCCGGCTGG AGGACAGCGA GGAGCGGCTG
    CGCAGGCAGC AGGAGGAGAA GGACAGCCAG ATGAAGAGCA TCATCAGCAG GCTAATGGCT
    GTGGAAGAGG AACTGAAGAA GGATCATGCT GAGATGCAGG CAGTTATTGA TGCAAAGCAA
    AAAATAATCG ATGCACAGGA AAAACGGATC GTGTCGCTGG ATTCGGCCAA CACCAGACTG
    ATGAGTGCGC TGACCCAGGT GAAGGAACGG TACAGCATGC AGGTCCGAAA TGGCATCTCC
    CCCACCAACC CCACCAAGCT TTCCATCACG GAGAATGGTG AATTCAAAAA CAGCAGCTGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27409
    Clone ID Related Accession (Same CDS sequence) XM_015466924.1 , XM_003587091.4
    Accession Version XM_003587091.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3843bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ras GTPase-activating protein nGAP isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104439.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003587091.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCTCT CCCTGTCGTC CGGAGGAGCC GCGGAGGCGC TGTCCTGGCC AGAGATGTTC 
    CCGGGATTGG AGTCCGACTC GCCGCTCCCC CCGGAGGAGC TGGACGCGGT TGTCCCGGTC
    AGTGGGGCCA TGGCCGGTGG CATGTTGGAT CGGATTCTTC TGGAGTCCGT GTGCCAGCAA
    CAGAGCTGGG TCCGCGTGTA CGATGTGAAA GGGCCACCTA CCCACCGTCT GTCTTGTGGC
    CAGTCGCCCT ACACCGACAC AACAACATGG GAGCGGAAGT ACTGCATCCT CACAGACAGC
    CAGCTGGTGT TGCTCAACAA GGAGAAGGAG ATACCTGTGG ACGGAGGACA AGAACAGCAG
    GCGGATTCCA CCAAAGGGCG ATGCCTACGG AGAACTGTCA GTGTTCCTTC CGAGGGTCAG
    TTTCCTGAAT ACCCACCAGA GGGCGCCACT AAACTGGAGG TGCCAGCAGA AAGGTCCCCA
    CGCAGACGGA GTATCTCAGG GACCACTACA TCTGAGAAAC CCAACTCTAT GGACACTGCG
    AATACCTCAC CCTTCAAAGT ACCAGGGTTC TTCAGCAAGC GCCTAAAAGG CTCCATCAAG
    AGGACCAAAA GCCAGTCAAA GCTTGACCGA AACACGAGCT TTCGGCTTCC CTCTCTTCGC
    AATACCGATG ACAGGTCTCG TGGGCTGCCT AAACTAAAAG AGTCCCGTTC TCATGAATCT
    TTGCTGAGCC CATGCAGTGC CGTGGAATGT CTGGATCTCG GTAGAGGGGA GCCTGTGTCG
    GTAAAGCCAC TCCATAGCAG CATCCTTGGA CAAGACTTCT GCTTTGAGGT TACCTACTTA
    AGTGGAAGTA AATGCTTCAG TTGTAACTCT GCTTCAGAGA GAGATAAGTG GATGGAAAAC
    CTGCGCAGGA CAGTTCAACC AAATAAGGAC AATTGTAGAC GAGCTGAAAA TGTTCTCCGT
    TTATGGATTA TTGAAGCCAA GGACCTTGCC CCTAAAAAGA AATATTTCTG TGAACTGTGC
    CTTGATGATA CCCTCTTTGC TCGTACAACT AGCAAGACCA AAGCAGACAA TATTTTCTGG
    GGTGAACATT TTGAGTTCTA CAGCCTTCCA CCTCTTCATA GCATCACAGT TCACATTTAT
    AAAGATGTGG AAAAAAAGAA GAAAAAAGAC AAGAATAATT ATGTAGGGCT GGTCAACATC
    CCCACTGCCA GTGTGACTGG TCGCCAGTTT GTAGAAAAGT GGTACCCAGT GAGCACACCT
    ACACCCAACA AAGGAAAGAC AGGAGGACCT TCTATTCGGA TTAAGTCGCG TTTCCAAACC
    ATCACCATTC TGCCTATGGA GCAATACAAA GAGTTTGCAG AATTTGTCAC CAGCAACTAT
    ACCATGTTAT GTTCTGTTCT TGAACCAGTA ATCAGTGTGA GAAATAAAGA AGAGCTAGCC
    TGTGCCTTAG TGCACATTCT TCAAAGCACT GGAAGGGCCA AGGATTTTCT GACGGACTTG
    GTGATGTCTG AGGTGGATCG TTGTGGAGAA CATGATGTCT TGATCTTCAG AGAGAACACC
    ATTGCCACCA AATCCATTGA GGAATACCTC AAGTTGGTGG GACAACAGTA TCTTCACGAT
    GCACTGGGGG AGTTCATCAA AGCGTTGTAT GAGTCAGATG AAAACTGTGA AGTGGATCCC
    AGCAAATGTT CATCCAGTGA ACTTCTGGAC CATCAGAGCA ACCTGAAAAT GTGCTGTGAG
    CTGGCTTTCT GCAAGATCAT CAACTCTTAC TGTGTTTTTC CTCGTGAGCT GAAAGAAGTG
    TTTGCATCCT GGAAGCAGCA GTGCCTGAAC CGCGGCAAGC AGGACATCAG CGAGAGGCTC
    ATCAGCGCCT CGCTGTTTCT CCGTTTTCTG TGCCCGGCCA TCATGTCTCC CAGTCTCTTC
    AACCTCATGC AGGAGTATCC TGATGACCGC ACATCTCGGA CTCTGACTCT CATTGCCAAG
    GTCATCCAGA ACCTGGCCAA CTTTGCCAAA TTTGGTAACA AGGAGGAATA CATGGCATTC
    ATGAATGATT TTTTAGAACA TGAATGGGGT GGAATGAAGC GCTTTCTTTT GGAGATCTCT
    AATCCAGACA CCATCTCAAA CACCCCAGGC TTTGATGGTT ACATTGATCT GGGCCGAGAG
    CTTTCAGTTT TGCATTCCTT ACTATGGGAA GTAGTTTCCC AACTTGATAA GGCAACCGTG
    GCAAAATTGG GACCTCTCCC TCGTGTTCTT GCTGATATTA CCAAATCTCT GACTAATCCT
    ACACCAATAC AACAGCAACT GAGACGCTTC ACTGAGCATA ACTCCAGTCC AAATGTCAGT
    GGAAGCCTCT CCTCTGGGCT GCAGAAAATA TTTGAAGACC CTACTGACAG TGATTTGCAC
    AAACTAAAAT CCCCAAGCCA GGACAACACA GACAGTTATT TCAGAGGGAA GACATTATTG
    CTGGTCCAGC AAGCCTCCTC CCAGAGCATG ACTTACTCGG AAAAGGATGA AAAGGAAAGT
    AGCCTCCCCA ATGGCCGCAG CATCTCCCTC ATGGACCTTC AGGAGACGCA CGCAGCCCAA
    GCAGAGCATC CATCTGTGAT ACTCGACGTG CCCATGCGCT TGACCGGAAG CCAGCTCTCC
    ATAACTCAGG TGGCCAGCAT CAAGCAACTG CGGGAGACCC AGAGCACCCC CCAGAGTGCA
    CCCCAAGTGC GAAGGCCCCT GCACCCAGCC TTGAACCAGC CAGGCAGCCT CCAGCCCCTG
    TCCTTCCAGA ACCCTGTCTA TCACCTCAGT AACCCCATTC CTGCAATGCC AAAGGCCTCT
    GTGGACTCCA GTTTGGAGAA CCTAAGCACT GCCAGTTCCA GGAGCCAAAG TAACAGTGAA
    GACTTCAAAC TCAGCGGACC CAGCAACAGC AGCATGGAGG ACTTCACCAA ACGGAGCACG
    CAGAGTGAGG ACTTCTCCAG GCGCCACACC ATGCCCGACA GACACATACC TCTCGCCCTG
    CCGCGGCAGA ACAGCACGGG GCAGACCCAG CTCCGGAAGA TGGACCCGGC GGGGCTGGGC
    GCCCGAGCCA AAGCCCCGCC GTCCCTGCCG CACAGCGCCT CCCTGCGGAG CACCGGGAGC
    ATGTCGGTGG CATCAGCGGC TCTGGTGGCC GAGCCCCTGC AGAACGGGAG CCGGTCCCGA
    CAGCAGTCCT CTTCCTCCAG AGAGAGCCCG GTCCCCAAAG TGAGAGCCAT CCAGAGGCAG
    CAGACCCAGC AGGTTCAGTC ACCTGTGGAC TCCGCCACAA TGTCCCCAGT CGAGAGGACA
    GCAGCCTGGG TCCTGAACAA TGGGCAGTAT GAAGAGGACG TGGAAGAGAC TGAGCAAAAT
    CAGGACGAAG CCAAGCATGC TGAGAAGTAC GAACAGGAAA TCACCAAACT GAAGGAGCGC
    CTGCGCGTGT CCAGCCGGCG ACTCGAGGAG TATGAGCGCC GGTTGCTAGT GCAGGAGCAG
    CAGATGCAGA AGCTGCTGCT GGAGTACAAG GCCCGGCTGG AGGACAGCGA GGAGCGGCTG
    CGCAGGCAGC AGGAGGAGAA GGACAGCCAG ATGAAGAGCA TCATCAGCAG GCTAATGGCT
    GTGGAAGAGG AACTGAAGAA GGATCATGCT GAGATGCAGG CAGTTATTGA TGCAAAGCAA
    AAAATAATCG ATGCACAGGA AAAACGGATC GTGTCGCTGG ATTCGGCCAA CACCAGACTG
    ATGAGTGCGC TGACCCAGGT GAAGGAACGG TACAGCATGC AGGTCCGAAA TGGCATCTCC
    CCCACCAACC CCACCAAGCT TTCCATCACG GAGAATGGTG AATTCAAAAA CAGCAGCTGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003587139.2
    CDS96..3938
    Translation

    Target ORF information:

    RefSeq Version XM_003587091.4
    Organism Bos taurus(cattle)
    Definition Bos taurus RAS protein activator like 2 (RASAL2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003587091.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCTCT CCCTGTCGTC CGGAGGAGCC GCGGAGGCGC TGTCCTGGCC AGAGATGTTC 
    CCGGGATTGG AGTCCGACTC GCCGCTCCCC CCGGAGGAGC TGGACGCGGT TGTCCCGGTC
    AGTGGGGCCA TGGCCGGTGG CATGTTGGAT CGGATTCTTC TGGAGTCCGT GTGCCAGCAA
    CAGAGCTGGG TCCGCGTGTA CGATGTGAAA GGGCCACCTA CCCACCGTCT GTCTTGTGGC
    CAGTCGCCCT ACACCGACAC AACAACATGG GAGCGGAAGT ACTGCATCCT CACAGACAGC
    CAGCTGGTGT TGCTCAACAA GGAGAAGGAG ATACCTGTGG ACGGAGGACA AGAACAGCAG
    GCGGATTCCA CCAAAGGGCG ATGCCTACGG AGAACTGTCA GTGTTCCTTC CGAGGGTCAG
    TTTCCTGAAT ACCCACCAGA GGGCGCCACT AAACTGGAGG TGCCAGCAGA AAGGTCCCCA
    CGCAGACGGA GTATCTCAGG GACCACTACA TCTGAGAAAC CCAACTCTAT GGACACTGCG
    AATACCTCAC CCTTCAAAGT ACCAGGGTTC TTCAGCAAGC GCCTAAAAGG CTCCATCAAG
    AGGACCAAAA GCCAGTCAAA GCTTGACCGA AACACGAGCT TTCGGCTTCC CTCTCTTCGC
    AATACCGATG ACAGGTCTCG TGGGCTGCCT AAACTAAAAG AGTCCCGTTC TCATGAATCT
    TTGCTGAGCC CATGCAGTGC CGTGGAATGT CTGGATCTCG GTAGAGGGGA GCCTGTGTCG
    GTAAAGCCAC TCCATAGCAG CATCCTTGGA CAAGACTTCT GCTTTGAGGT TACCTACTTA
    AGTGGAAGTA AATGCTTCAG TTGTAACTCT GCTTCAGAGA GAGATAAGTG GATGGAAAAC
    CTGCGCAGGA CAGTTCAACC AAATAAGGAC AATTGTAGAC GAGCTGAAAA TGTTCTCCGT
    TTATGGATTA TTGAAGCCAA GGACCTTGCC CCTAAAAAGA AATATTTCTG TGAACTGTGC
    CTTGATGATA CCCTCTTTGC TCGTACAACT AGCAAGACCA AAGCAGACAA TATTTTCTGG
    GGTGAACATT TTGAGTTCTA CAGCCTTCCA CCTCTTCATA GCATCACAGT TCACATTTAT
    AAAGATGTGG AAAAAAAGAA GAAAAAAGAC AAGAATAATT ATGTAGGGCT GGTCAACATC
    CCCACTGCCA GTGTGACTGG TCGCCAGTTT GTAGAAAAGT GGTACCCAGT GAGCACACCT
    ACACCCAACA AAGGAAAGAC AGGAGGACCT TCTATTCGGA TTAAGTCGCG TTTCCAAACC
    ATCACCATTC TGCCTATGGA GCAATACAAA GAGTTTGCAG AATTTGTCAC CAGCAACTAT
    ACCATGTTAT GTTCTGTTCT TGAACCAGTA ATCAGTGTGA GAAATAAAGA AGAGCTAGCC
    TGTGCCTTAG TGCACATTCT TCAAAGCACT GGAAGGGCCA AGGATTTTCT GACGGACTTG
    GTGATGTCTG AGGTGGATCG TTGTGGAGAA CATGATGTCT TGATCTTCAG AGAGAACACC
    ATTGCCACCA AATCCATTGA GGAATACCTC AAGTTGGTGG GACAACAGTA TCTTCACGAT
    GCACTGGGGG AGTTCATCAA AGCGTTGTAT GAGTCAGATG AAAACTGTGA AGTGGATCCC
    AGCAAATGTT CATCCAGTGA ACTTCTGGAC CATCAGAGCA ACCTGAAAAT GTGCTGTGAG
    CTGGCTTTCT GCAAGATCAT CAACTCTTAC TGTGTTTTTC CTCGTGAGCT GAAAGAAGTG
    TTTGCATCCT GGAAGCAGCA GTGCCTGAAC CGCGGCAAGC AGGACATCAG CGAGAGGCTC
    ATCAGCGCCT CGCTGTTTCT CCGTTTTCTG TGCCCGGCCA TCATGTCTCC CAGTCTCTTC
    AACCTCATGC AGGAGTATCC TGATGACCGC ACATCTCGGA CTCTGACTCT CATTGCCAAG
    GTCATCCAGA ACCTGGCCAA CTTTGCCAAA TTTGGTAACA AGGAGGAATA CATGGCATTC
    ATGAATGATT TTTTAGAACA TGAATGGGGT GGAATGAAGC GCTTTCTTTT GGAGATCTCT
    AATCCAGACA CCATCTCAAA CACCCCAGGC TTTGATGGTT ACATTGATCT GGGCCGAGAG
    CTTTCAGTTT TGCATTCCTT ACTATGGGAA GTAGTTTCCC AACTTGATAA GGCAACCGTG
    GCAAAATTGG GACCTCTCCC TCGTGTTCTT GCTGATATTA CCAAATCTCT GACTAATCCT
    ACACCAATAC AACAGCAACT GAGACGCTTC ACTGAGCATA ACTCCAGTCC AAATGTCAGT
    GGAAGCCTCT CCTCTGGGCT GCAGAAAATA TTTGAAGACC CTACTGACAG TGATTTGCAC
    AAACTAAAAT CCCCAAGCCA GGACAACACA GACAGTTATT TCAGAGGGAA GACATTATTG
    CTGGTCCAGC AAGCCTCCTC CCAGAGCATG ACTTACTCGG AAAAGGATGA AAAGGAAAGT
    AGCCTCCCCA ATGGCCGCAG CATCTCCCTC ATGGACCTTC AGGAGACGCA CGCAGCCCAA
    GCAGAGCATC CATCTGTGAT ACTCGACGTG CCCATGCGCT TGACCGGAAG CCAGCTCTCC
    ATAACTCAGG TGGCCAGCAT CAAGCAACTG CGGGAGACCC AGAGCACCCC CCAGAGTGCA
    CCCCAAGTGC GAAGGCCCCT GCACCCAGCC TTGAACCAGC CAGGCAGCCT CCAGCCCCTG
    TCCTTCCAGA ACCCTGTCTA TCACCTCAGT AACCCCATTC CTGCAATGCC AAAGGCCTCT
    GTGGACTCCA GTTTGGAGAA CCTAAGCACT GCCAGTTCCA GGAGCCAAAG TAACAGTGAA
    GACTTCAAAC TCAGCGGACC CAGCAACAGC AGCATGGAGG ACTTCACCAA ACGGAGCACG
    CAGAGTGAGG ACTTCTCCAG GCGCCACACC ATGCCCGACA GACACATACC TCTCGCCCTG
    CCGCGGCAGA ACAGCACGGG GCAGACCCAG CTCCGGAAGA TGGACCCGGC GGGGCTGGGC
    GCCCGAGCCA AAGCCCCGCC GTCCCTGCCG CACAGCGCCT CCCTGCGGAG CACCGGGAGC
    ATGTCGGTGG CATCAGCGGC TCTGGTGGCC GAGCCCCTGC AGAACGGGAG CCGGTCCCGA
    CAGCAGTCCT CTTCCTCCAG AGAGAGCCCG GTCCCCAAAG TGAGAGCCAT CCAGAGGCAG
    CAGACCCAGC AGGTTCAGTC ACCTGTGGAC TCCGCCACAA TGTCCCCAGT CGAGAGGACA
    GCAGCCTGGG TCCTGAACAA TGGGCAGTAT GAAGAGGACG TGGAAGAGAC TGAGCAAAAT
    CAGGACGAAG CCAAGCATGC TGAGAAGTAC GAACAGGAAA TCACCAAACT GAAGGAGCGC
    CTGCGCGTGT CCAGCCGGCG ACTCGAGGAG TATGAGCGCC GGTTGCTAGT GCAGGAGCAG
    CAGATGCAGA AGCTGCTGCT GGAGTACAAG GCCCGGCTGG AGGACAGCGA GGAGCGGCTG
    CGCAGGCAGC AGGAGGAGAA GGACAGCCAG ATGAAGAGCA TCATCAGCAG GCTAATGGCT
    GTGGAAGAGG AACTGAAGAA GGATCATGCT GAGATGCAGG CAGTTATTGA TGCAAAGCAA
    AAAATAATCG ATGCACAGGA AAAACGGATC GTGTCGCTGG ATTCGGCCAA CACCAGACTG
    ATGAGTGCGC TGACCCAGGT GAAGGAACGG TACAGCATGC AGGTCCGAAA TGGCATCTCC
    CCCACCAACC CCACCAAGCT TTCCATCACG GAGAATGGTG AATTCAAAAA CAGCAGCTGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27404
    Clone ID Related Accession (Same CDS sequence) XM_005217238.3 , XM_005194886.3
    Accession Version XM_005217238.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4272bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ras GTPase-activating protein nGAP isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104439.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005217238.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAGTCCT GGAAGATGAG CATCTTTAAG AGACAGACAT CACCGTGTCC TCCAAGCTAT 
    CGAAGATCTT TGTCAGAACC TGCTTATACA CACTCAGAGA TAGGAACAAG CTTAAAGAGT
    TATCCATGGC GTGGTCCAGT GAAAAGCAAG AGGAAGAAAA ATGAACAGGC TGTTTCCAGT
    CTAGGAAAGC CACTGGGTGC TTATCGGTGG CAAACACACC ACAGCTTAAG GTTGAAAGGG
    GACAAAAAGG AGCAATTGGA AAATAATAAA GGAGACTTGG ATGAACCACC ACAACAAGCA
    GTCCTGCCAA AGATGGTTGG GGATAATATT TCTTCCTCTG GCAATGAAGT CCTTGGGACA
    TCAATGGAGA AGCAGAACCT GGTAAACACT CAGTCACTTC GGGTTCCATT CAGCAGATCT
    CTTTCAGAGC CAGAACCCGG CTCCAGTGTC AGATTTGTTA GACGGTGGCC AACTTCAGTG
    GGGTCAGTGG AGTCTGATCA GCAGGGATAT TTCATCCGTG GCATTTTCTC TACTCTTTCC
    AATGGCTTCT CCAGGATGCT GAGTCTAAAA GGAGGGTCAA CCAGTGAGCC AGTAAGAGAA
    GATGTGAAAG GGCCACCTAC CCACCGTCTG TCTTGTGGCC AGTCGCCCTA CACCGACACA
    ACAACATGGG AGCGGAAGTA CTGCATCCTC ACAGACAGCC AGCTGGTGTT GCTCAACAAG
    GAGAAGGAGA TACCTGTGGA CGGAGGACAA GAACAGCAGG CGGATTCCAC CAAAGGGCGA
    TGCCTACGGA GAACTGTCAG TGTTCCTTCC GAGGGTCAGT TTCCTGAATA CCCACCAGAG
    GGCGCCACTA AACTGGAGGT GCCAGCAGAA AGGTCCCCAC GCAGACGGAG TATCTCAGGG
    ACCACTACAT CTGAGAAACC CAACTCTATG GACACTGCGA ATACCTCACC CTTCAAAGTA
    CCAGGGTTCT TCAGCAAGCG CCTAAAAGGC TCCATCAAGA GGACCAAAAG CCAGTCAAAG
    CTTGACCGAA ACACGAGCTT TCGGCTTCCC TCTCTTCGCA ATACCGATGA CAGGTCTCGT
    GGGCTGCCTA AACTAAAAGA GTCCCGTTCT CATGAATCTT TGCTGAGCCC ATGCAGTGCC
    GTGGAATGTC TGGATCTCGG TAGAGGGGAG CCTGTGTCGG TAAAGCCACT CCATAGCAGC
    ATCCTTGGAC AAGACTTCTG CTTTGAGGTT ACCTACTTAA GTGGAAGTAA ATGCTTCAGT
    TGTAACTCTG CTTCAGAGAG AGATAAGTGG ATGGAAAACC TGCGCAGGAC AGTTCAACCA
    AATAAGGACA ATTGTAGACG AGCTGAAAAT GTTCTCCGTT TATGGATTAT TGAAGCCAAG
    GACCTTGCCC CTAAAAAGAA ATATTTCTGT GAACTGTGCC TTGATGATAC CCTCTTTGCT
    CGTACAACTA GCAAGACCAA AGCAGACAAT ATTTTCTGGG GTGAACATTT TGAGTTCTAC
    AGCCTTCCAC CTCTTCATAG CATCACAGTT CACATTTATA AAGATGTGGA AAAAAAGAAG
    AAAAAAGACA AGAATAATTA TGTAGGGCTG GTCAACATCC CCACTGCCAG TGTGACTGGT
    CGCCAGTTTG TAGAAAAGTG GTACCCAGTG AGCACACCTA CACCCAACAA AGGAAAGACA
    GGAGGACCTT CTATTCGGAT TAAGTCGCGT TTCCAAACCA TCACCATTCT GCCTATGGAG
    CAATACAAAG AGTTTGCAGA ATTTGTCACC AGCAACTATA CCATGTTATG TTCTGTTCTT
    GAACCAGTAA TCAGTGTGAG AAATAAAGAA GAGCTAGCCT GTGCCTTAGT GCACATTCTT
    CAAAGCACTG GAAGGGCCAA GGATTTTCTG ACGGACTTGG TGATGTCTGA GGTGGATCGT
    TGTGGAGAAC ATGATGTCTT GATCTTCAGA GAGAACACCA TTGCCACCAA ATCCATTGAG
    GAATACCTCA AGTTGGTGGG ACAACAGTAT CTTCACGATG CACTGGGGGA GTTCATCAAA
    GCGTTGTATG AGTCAGATGA AAACTGTGAA GTGGATCCCA GCAAATGTTC ATCCAGTGAA
    CTTCTGGACC ATCAGAGCAA CCTGAAAATG TGCTGTGAGC TGGCTTTCTG CAAGATCATC
    AACTCTTACT GTGTTTTTCC TCGTGAGCTG AAAGAAGTGT TTGCATCCTG GAAGCAGCAG
    TGCCTGAACC GCGGCAAGCA GGACATCAGC GAGAGGCTCA TCAGCGCCTC GCTGTTTCTC
    CGTTTTCTGT GCCCGGCCAT CATGTCTCCC AGTCTCTTCA ACCTCATGCA GGAGTATCCT
    GATGACCGCA CATCTCGGAC TCTGACTCTC ATTGCCAAGG TCATCCAGAA CCTGGCCAAC
    TTTGCCAAAT TTGGTAACAA GGAGGAATAC ATGGCATTCA TGAATGATTT TTTAGAACAT
    GAATGGGGTG GAATGAAGCG CTTTCTTTTG GAGATCTCTA ATCCAGACAC CATCTCAAAC
    ACCCCAGGCT TTGATGGTTA CATTGATCTG GGCCGAGAGC TTTCAGTTTT GCATTCCTTA
    CTATGGGAAG TAGTTTCCCA ACTTGATAAG GGTGAAAATT CCTTCCTACA GGCAACCGTG
    GCAAAATTGG GACCTCTCCC TCGTGTTCTT GCTGATATTA CCAAATCTCT GACTAATCCT
    ACACCAATAC AACAGCAACT GAGACGCTTC ACTGAGCATA ACTCCAGTCC AAATGTCAGT
    GGAAGCCTCT CCTCTGGGCT GCAGAAAATA TTTGAAGACC CTACTGACAG TGATTTGCAC
    AAACTAAAAT CCCCAAGCCA GGACAACACA GACAGTTATT TCAGAGGGAA GACATTATTG
    CTGGTCCAGC AAGCCTCCTC CCAGAGCATG ACTTACTCGG AAAAGGATGA AAAGGAAAGT
    AGCCTCCCCA ATGGCCGCAG CATCTCCCTC ATGGACCTTC AGGAGACGCA CGCAGCCCAA
    GCAGAGCATC CATCTGTGAT ACTCGACGTG CCCATGCGCT TGACCGGAAG CCAGCTCTCC
    ATAACTCAGG TGGCCAGCAT CAAGCAACTG CGGGAGACCC AGAGCACCCC CCAGAGTGCA
    CCCCAAGTGC GAAGGCCCCT GCACCCAGCC TTGAACCAGC CAGGCAGCCT CCAGCCCCTG
    TCCTTCCAGA ACCCTGTCTA TCACCTCAGT AACCCCATTC CTGCAATGCC AAAGGCCTCT
    GTGGACTCCA GTTTGGAGAA CCTAAGCACT GCCAGTTCCA GGAGCCAAAG TAACAGTGAA
    GACTTCAAAC TCAGCGGACC CAGCAACAGC AGCATGGAGG ACTTCACCAA ACGGAGCACG
    CAGAGTGAGG ACTTCTCCAG GCGCCACACC ATGCCCGACA GACACATACC TCTCGCCCTG
    CCGCGGCAGA ACAGCACGGG GCAGACCCAG CTCCGGAAGA TGGACCCGGC GGGGCTGGGC
    GCCCGAGCCA AAGCCCCGCC GTCCCTGCCG CACAGCGCCT CCCTGCGGAG CACCGGGAGC
    ATGTCGGTGG CATCAGCGGC TCTGGTGGCC GAGCCCCTGC AGAACGGGAG CCGGTCCCGA
    CAGCAGTCCT CTTCCTCCAG AGAGAGCCCG GTCCCCAAAG TGAGAGCCAT CCAGAGGCAG
    CAGACCCAGC AGGTTCAGTC ACCTGTGGAC TCCGCCACAA TGTCCCCAGT CGAGAGGACA
    GCAGCCTGGG TCCTGAACAA TGGGCAGTAT GAAGAGGACG TGGAAGAGAC TGAGCAAAAT
    CAGGACGAAG CCAAGCATGC TGAGAAGTAC GAACAGGAAA TCACCAAACT GAAGGAGCGC
    CTGCGCGTGT CCAGCCGGCG ACTCGAGGAG TATGAGCGCC GGTTGCTAGT GCAGGAGCAG
    CAGATGCAGA AGCTGCTGCT GGAGTACAAG GCCCGGCTGG AGGACAGCGA GGAGCGGCTG
    CGCAGGCAGC AGGAGGAGAA GGACAGCCAG ATGAAGAGCA TCATCAGCAG GCTAATGGCT
    GTGGAAGAGG AACTGAAGAA GGATCATGCT GAGATGCAGG CAGTTATTGA TGCAAAGCAA
    AAAATAATCG ATGCACAGGT CATAAATGGA GATGAGATTA TGCAAACAGG AAAAACGGAT
    CGTGTCGCTG GATTCGGCCA ACACCAGACT GATGAGTGCG CTGACCCAGG TGAAGGAACG
    GTACAGCATG CAGGTCCGAA ATGGCATCTC CCCCACCAAC CCCACCAAGC TTTCCATCAC
    GGAGAATGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005217295.1
    CDS297..4568
    Translation

    Target ORF information:

    RefSeq Version XM_005217238.3
    Organism Bos taurus(cattle)
    Definition Bos taurus RAS protein activator like 2 (RASAL2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005217238.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAGTCCT GGAAGATGAG CATCTTTAAG AGACAGACAT CACCGTGTCC TCCAAGCTAT 
    CGAAGATCTT TGTCAGAACC TGCTTATACA CACTCAGAGA TAGGAACAAG CTTAAAGAGT
    TATCCATGGC GTGGTCCAGT GAAAAGCAAG AGGAAGAAAA ATGAACAGGC TGTTTCCAGT
    CTAGGAAAGC CACTGGGTGC TTATCGGTGG CAAACACACC ACAGCTTAAG GTTGAAAGGG
    GACAAAAAGG AGCAATTGGA AAATAATAAA GGAGACTTGG ATGAACCACC ACAACAAGCA
    GTCCTGCCAA AGATGGTTGG GGATAATATT TCTTCCTCTG GCAATGAAGT CCTTGGGACA
    TCAATGGAGA AGCAGAACCT GGTAAACACT CAGTCACTTC GGGTTCCATT CAGCAGATCT
    CTTTCAGAGC CAGAACCCGG CTCCAGTGTC AGATTTGTTA GACGGTGGCC AACTTCAGTG
    GGGTCAGTGG AGTCTGATCA GCAGGGATAT TTCATCCGTG GCATTTTCTC TACTCTTTCC
    AATGGCTTCT CCAGGATGCT GAGTCTAAAA GGAGGGTCAA CCAGTGAGCC AGTAAGAGAA
    GATGTGAAAG GGCCACCTAC CCACCGTCTG TCTTGTGGCC AGTCGCCCTA CACCGACACA
    ACAACATGGG AGCGGAAGTA CTGCATCCTC ACAGACAGCC AGCTGGTGTT GCTCAACAAG
    GAGAAGGAGA TACCTGTGGA CGGAGGACAA GAACAGCAGG CGGATTCCAC CAAAGGGCGA
    TGCCTACGGA GAACTGTCAG TGTTCCTTCC GAGGGTCAGT TTCCTGAATA CCCACCAGAG
    GGCGCCACTA AACTGGAGGT GCCAGCAGAA AGGTCCCCAC GCAGACGGAG TATCTCAGGG
    ACCACTACAT CTGAGAAACC CAACTCTATG GACACTGCGA ATACCTCACC CTTCAAAGTA
    CCAGGGTTCT TCAGCAAGCG CCTAAAAGGC TCCATCAAGA GGACCAAAAG CCAGTCAAAG
    CTTGACCGAA ACACGAGCTT TCGGCTTCCC TCTCTTCGCA ATACCGATGA CAGGTCTCGT
    GGGCTGCCTA AACTAAAAGA GTCCCGTTCT CATGAATCTT TGCTGAGCCC ATGCAGTGCC
    GTGGAATGTC TGGATCTCGG TAGAGGGGAG CCTGTGTCGG TAAAGCCACT CCATAGCAGC
    ATCCTTGGAC AAGACTTCTG CTTTGAGGTT ACCTACTTAA GTGGAAGTAA ATGCTTCAGT
    TGTAACTCTG CTTCAGAGAG AGATAAGTGG ATGGAAAACC TGCGCAGGAC AGTTCAACCA
    AATAAGGACA ATTGTAGACG AGCTGAAAAT GTTCTCCGTT TATGGATTAT TGAAGCCAAG
    GACCTTGCCC CTAAAAAGAA ATATTTCTGT GAACTGTGCC TTGATGATAC CCTCTTTGCT
    CGTACAACTA GCAAGACCAA AGCAGACAAT ATTTTCTGGG GTGAACATTT TGAGTTCTAC
    AGCCTTCCAC CTCTTCATAG CATCACAGTT CACATTTATA AAGATGTGGA AAAAAAGAAG
    AAAAAAGACA AGAATAATTA TGTAGGGCTG GTCAACATCC CCACTGCCAG TGTGACTGGT
    CGCCAGTTTG TAGAAAAGTG GTACCCAGTG AGCACACCTA CACCCAACAA AGGAAAGACA
    GGAGGACCTT CTATTCGGAT TAAGTCGCGT TTCCAAACCA TCACCATTCT GCCTATGGAG
    CAATACAAAG AGTTTGCAGA ATTTGTCACC AGCAACTATA CCATGTTATG TTCTGTTCTT
    GAACCAGTAA TCAGTGTGAG AAATAAAGAA GAGCTAGCCT GTGCCTTAGT GCACATTCTT
    CAAAGCACTG GAAGGGCCAA GGATTTTCTG ACGGACTTGG TGATGTCTGA GGTGGATCGT
    TGTGGAGAAC ATGATGTCTT GATCTTCAGA GAGAACACCA TTGCCACCAA ATCCATTGAG
    GAATACCTCA AGTTGGTGGG ACAACAGTAT CTTCACGATG CACTGGGGGA GTTCATCAAA
    GCGTTGTATG AGTCAGATGA AAACTGTGAA GTGGATCCCA GCAAATGTTC ATCCAGTGAA
    CTTCTGGACC ATCAGAGCAA CCTGAAAATG TGCTGTGAGC TGGCTTTCTG CAAGATCATC
    AACTCTTACT GTGTTTTTCC TCGTGAGCTG AAAGAAGTGT TTGCATCCTG GAAGCAGCAG
    TGCCTGAACC GCGGCAAGCA GGACATCAGC GAGAGGCTCA TCAGCGCCTC GCTGTTTCTC
    CGTTTTCTGT GCCCGGCCAT CATGTCTCCC AGTCTCTTCA ACCTCATGCA GGAGTATCCT
    GATGACCGCA CATCTCGGAC TCTGACTCTC ATTGCCAAGG TCATCCAGAA CCTGGCCAAC
    TTTGCCAAAT TTGGTAACAA GGAGGAATAC ATGGCATTCA TGAATGATTT TTTAGAACAT
    GAATGGGGTG GAATGAAGCG CTTTCTTTTG GAGATCTCTA ATCCAGACAC CATCTCAAAC
    ACCCCAGGCT TTGATGGTTA CATTGATCTG GGCCGAGAGC TTTCAGTTTT GCATTCCTTA
    CTATGGGAAG TAGTTTCCCA ACTTGATAAG GGTGAAAATT CCTTCCTACA GGCAACCGTG
    GCAAAATTGG GACCTCTCCC TCGTGTTCTT GCTGATATTA CCAAATCTCT GACTAATCCT
    ACACCAATAC AACAGCAACT GAGACGCTTC ACTGAGCATA ACTCCAGTCC AAATGTCAGT
    GGAAGCCTCT CCTCTGGGCT GCAGAAAATA TTTGAAGACC CTACTGACAG TGATTTGCAC
    AAACTAAAAT CCCCAAGCCA GGACAACACA GACAGTTATT TCAGAGGGAA GACATTATTG
    CTGGTCCAGC AAGCCTCCTC CCAGAGCATG ACTTACTCGG AAAAGGATGA AAAGGAAAGT
    AGCCTCCCCA ATGGCCGCAG CATCTCCCTC ATGGACCTTC AGGAGACGCA CGCAGCCCAA
    GCAGAGCATC CATCTGTGAT ACTCGACGTG CCCATGCGCT TGACCGGAAG CCAGCTCTCC
    ATAACTCAGG TGGCCAGCAT CAAGCAACTG CGGGAGACCC AGAGCACCCC CCAGAGTGCA
    CCCCAAGTGC GAAGGCCCCT GCACCCAGCC TTGAACCAGC CAGGCAGCCT CCAGCCCCTG
    TCCTTCCAGA ACCCTGTCTA TCACCTCAGT AACCCCATTC CTGCAATGCC AAAGGCCTCT
    GTGGACTCCA GTTTGGAGAA CCTAAGCACT GCCAGTTCCA GGAGCCAAAG TAACAGTGAA
    GACTTCAAAC TCAGCGGACC CAGCAACAGC AGCATGGAGG ACTTCACCAA ACGGAGCACG
    CAGAGTGAGG ACTTCTCCAG GCGCCACACC ATGCCCGACA GACACATACC TCTCGCCCTG
    CCGCGGCAGA ACAGCACGGG GCAGACCCAG CTCCGGAAGA TGGACCCGGC GGGGCTGGGC
    GCCCGAGCCA AAGCCCCGCC GTCCCTGCCG CACAGCGCCT CCCTGCGGAG CACCGGGAGC
    ATGTCGGTGG CATCAGCGGC TCTGGTGGCC GAGCCCCTGC AGAACGGGAG CCGGTCCCGA
    CAGCAGTCCT CTTCCTCCAG AGAGAGCCCG GTCCCCAAAG TGAGAGCCAT CCAGAGGCAG
    CAGACCCAGC AGGTTCAGTC ACCTGTGGAC TCCGCCACAA TGTCCCCAGT CGAGAGGACA
    GCAGCCTGGG TCCTGAACAA TGGGCAGTAT GAAGAGGACG TGGAAGAGAC TGAGCAAAAT
    CAGGACGAAG CCAAGCATGC TGAGAAGTAC GAACAGGAAA TCACCAAACT GAAGGAGCGC
    CTGCGCGTGT CCAGCCGGCG ACTCGAGGAG TATGAGCGCC GGTTGCTAGT GCAGGAGCAG
    CAGATGCAGA AGCTGCTGCT GGAGTACAAG GCCCGGCTGG AGGACAGCGA GGAGCGGCTG
    CGCAGGCAGC AGGAGGAGAA GGACAGCCAG ATGAAGAGCA TCATCAGCAG GCTAATGGCT
    GTGGAAGAGG AACTGAAGAA GGATCATGCT GAGATGCAGG CAGTTATTGA TGCAAAGCAA
    AAAATAATCG ATGCACAGGT CATAAATGGA GATGAGATTA TGCAAACAGG AAAAACGGAT
    CGTGTCGCTG GATTCGGCCA ACACCAGACT GATGAGTGCG CTGACCCAGG TGAAGGAACG
    GTACAGCATG CAGGTCCGAA ATGGCATCTC CCCCACCAAC CCCACCAAGC TTTCCATCAC
    GGAGAATGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27404
    Clone ID Related Accession (Same CDS sequence) XM_005217238.3 , XM_005194886.3
    Accession Version XM_005194886.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4272bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ras GTPase-activating protein nGAP isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646324.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005194886.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAGTCCT GGAAGATGAG CATCTTTAAG AGACAGACAT CACCGTGTCC TCCAAGCTAT 
    CGAAGATCTT TGTCAGAACC TGCTTATACA CACTCAGAGA TAGGAACAAG CTTAAAGAGT
    TATCCATGGC GTGGTCCAGT GAAAAGCAAG AGGAAGAAAA ATGAACAGGC TGTTTCCAGT
    CTAGGAAAGC CACTGGGTGC TTATCGGTGG CAAACACACC ACAGCTTAAG GTTGAAAGGG
    GACAAAAAGG AGCAATTGGA AAATAATAAA GGAGACTTGG ATGAACCACC ACAACAAGCA
    GTCCTGCCAA AGATGGTTGG GGATAATATT TCTTCCTCTG GCAATGAAGT CCTTGGGACA
    TCAATGGAGA AGCAGAACCT GGTAAACACT CAGTCACTTC GGGTTCCATT CAGCAGATCT
    CTTTCAGAGC CAGAACCCGG CTCCAGTGTC AGATTTGTTA GACGGTGGCC AACTTCAGTG
    GGGTCAGTGG AGTCTGATCA GCAGGGATAT TTCATCCGTG GCATTTTCTC TACTCTTTCC
    AATGGCTTCT CCAGGATGCT GAGTCTAAAA GGAGGGTCAA CCAGTGAGCC AGTAAGAGAA
    GATGTGAAAG GGCCACCTAC CCACCGTCTG TCTTGTGGCC AGTCGCCCTA CACCGACACA
    ACAACATGGG AGCGGAAGTA CTGCATCCTC ACAGACAGCC AGCTGGTGTT GCTCAACAAG
    GAGAAGGAGA TACCTGTGGA CGGAGGACAA GAACAGCAGG CGGATTCCAC CAAAGGGCGA
    TGCCTACGGA GAACTGTCAG TGTTCCTTCC GAGGGTCAGT TTCCTGAATA CCCACCAGAG
    GGCGCCACTA AACTGGAGGT GCCAGCAGAA AGGTCCCCAC GCAGACGGAG TATCTCAGGG
    ACCACTACAT CTGAGAAACC CAACTCTATG GACACTGCGA ATACCTCACC CTTCAAAGTA
    CCAGGGTTCT TCAGCAAGCG CCTAAAAGGC TCCATCAAGA GGACCAAAAG CCAGTCAAAG
    CTTGACCGAA ACACGAGCTT TCGGCTTCCC TCTCTTCGCA ATACCGATGA CAGGTCTCGT
    GGGCTGCCTA AACTAAAAGA GTCCCGTTCT CATGAATCTT TGCTGAGCCC ATGCAGTGCC
    GTGGAATGTC TGGATCTCGG TAGAGGGGAG CCTGTGTCGG TAAAGCCACT CCATAGCAGC
    ATCCTTGGAC AAGACTTCTG CTTTGAGGTT ACCTACTTAA GTGGAAGTAA ATGCTTCAGT
    TGTAACTCTG CTTCAGAGAG AGATAAGTGG ATGGAAAACC TGCGCAGGAC AGTTCAACCA
    AATAAGGACA ATTGTAGACG AGCTGAAAAT GTTCTCCGTT TATGGATTAT TGAAGCCAAG
    GACCTTGCCC CTAAAAAGAA ATATTTCTGT GAACTGTGCC TTGATGATAC CCTCTTTGCT
    CGTACAACTA GCAAGACCAA AGCAGACAAT ATTTTCTGGG GTGAACATTT TGAGTTCTAC
    AGCCTTCCAC CTCTTCATAG CATCACAGTT CACATTTATA AAGATGTGGA AAAAAAGAAG
    AAAAAAGACA AGAATAATTA TGTAGGGCTG GTCAACATCC CCACTGCCAG TGTGACTGGT
    CGCCAGTTTG TAGAAAAGTG GTACCCAGTG AGCACACCTA CACCCAACAA AGGAAAGACA
    GGAGGACCTT CTATTCGGAT TAAGTCGCGT TTCCAAACCA TCACCATTCT GCCTATGGAG
    CAATACAAAG AGTTTGCAGA ATTTGTCACC AGCAACTATA CCATGTTATG TTCTGTTCTT
    GAACCAGTAA TCAGTGTGAG AAATAAAGAA GAGCTAGCCT GTGCCTTAGT GCACATTCTT
    CAAAGCACTG GAAGGGCCAA GGATTTTCTG ACGGACTTGG TGATGTCTGA GGTGGATCGT
    TGTGGAGAAC ATGATGTCTT GATCTTCAGA GAGAACACCA TTGCCACCAA ATCCATTGAG
    GAATACCTCA AGTTGGTGGG ACAACAGTAT CTTCACGATG CACTGGGGGA GTTCATCAAA
    GCGTTGTATG AGTCAGATGA AAACTGTGAA GTGGATCCCA GCAAATGTTC ATCCAGTGAA
    CTTCTGGACC ATCAGAGCAA CCTGAAAATG TGCTGTGAGC TGGCTTTCTG CAAGATCATC
    AACTCTTACT GTGTTTTTCC TCGTGAGCTG AAAGAAGTGT TTGCATCCTG GAAGCAGCAG
    TGCCTGAACC GCGGCAAGCA GGACATCAGC GAGAGGCTCA TCAGCGCCTC GCTGTTTCTC
    CGTTTTCTGT GCCCGGCCAT CATGTCTCCC AGTCTCTTCA ACCTCATGCA GGAGTATCCT
    GATGACCGCA CATCTCGGAC TCTGACTCTC ATTGCCAAGG TCATCCAGAA CCTGGCCAAC
    TTTGCCAAAT TTGGTAACAA GGAGGAATAC ATGGCATTCA TGAATGATTT TTTAGAACAT
    GAATGGGGTG GAATGAAGCG CTTTCTTTTG GAGATCTCTA ATCCAGACAC CATCTCAAAC
    ACCCCAGGCT TTGATGGTTA CATTGATCTG GGCCGAGAGC TTTCAGTTTT GCATTCCTTA
    CTATGGGAAG TAGTTTCCCA ACTTGATAAG GGTGAAAATT CCTTCCTACA GGCAACCGTG
    GCAAAATTGG GACCTCTCCC TCGTGTTCTT GCTGATATTA CCAAATCTCT GACTAATCCT
    ACACCAATAC AACAGCAACT GAGACGCTTC ACTGAGCATA ACTCCAGTCC AAATGTCAGT
    GGAAGCCTCT CCTCTGGGCT GCAGAAAATA TTTGAAGACC CTACTGACAG TGATTTGCAC
    AAACTAAAAT CCCCAAGCCA GGACAACACA GACAGTTATT TCAGAGGGAA GACATTATTG
    CTGGTCCAGC AAGCCTCCTC CCAGAGCATG ACTTACTCGG AAAAGGATGA AAAGGAAAGT
    AGCCTCCCCA ATGGCCGCAG CATCTCCCTC ATGGACCTTC AGGAGACGCA CGCAGCCCAA
    GCAGAGCATC CATCTGTGAT ACTCGACGTG CCCATGCGCT TGACCGGAAG CCAGCTCTCC
    ATAACTCAGG TGGCCAGCAT CAAGCAACTG CGGGAGACCC AGAGCACCCC CCAGAGTGCA
    CCCCAAGTGC GAAGGCCCCT GCACCCAGCC TTGAACCAGC CAGGCAGCCT CCAGCCCCTG
    TCCTTCCAGA ACCCTGTCTA TCACCTCAGT AACCCCATTC CTGCAATGCC AAAGGCCTCT
    GTGGACTCCA GTTTGGAGAA CCTAAGCACT GCCAGTTCCA GGAGCCAAAG TAACAGTGAA
    GACTTCAAAC TCAGCGGACC CAGCAACAGC AGCATGGAGG ACTTCACCAA ACGGAGCACG
    CAGAGTGAGG ACTTCTCCAG GCGCCACACC ATGCCCGACA GACACATACC TCTCGCCCTG
    CCGCGGCAGA ACAGCACGGG GCAGACCCAG CTCCGGAAGA TGGACCCGGC GGGGCTGGGC
    GCCCGAGCCA AAGCCCCGCC GTCCCTGCCG CACAGCGCCT CCCTGCGGAG CACCGGGAGC
    ATGTCGGTGG CATCAGCGGC TCTGGTGGCC GAGCCCCTGC AGAACGGGAG CCGGTCCCGA
    CAGCAGTCCT CTTCCTCCAG AGAGAGCCCG GTCCCCAAAG TGAGAGCCAT CCAGAGGCAG
    CAGACCCAGC AGGTTCAGTC ACCTGTGGAC TCCGCCACAA TGTCCCCAGT CGAGAGGACA
    GCAGCCTGGG TCCTGAACAA TGGGCAGTAT GAAGAGGACG TGGAAGAGAC TGAGCAAAAT
    CAGGACGAAG CCAAGCATGC TGAGAAGTAC GAACAGGAAA TCACCAAACT GAAGGAGCGC
    CTGCGCGTGT CCAGCCGGCG ACTCGAGGAG TATGAGCGCC GGTTGCTAGT GCAGGAGCAG
    CAGATGCAGA AGCTGCTGCT GGAGTACAAG GCCCGGCTGG AGGACAGCGA GGAGCGGCTG
    CGCAGGCAGC AGGAGGAGAA GGACAGCCAG ATGAAGAGCA TCATCAGCAG GCTAATGGCT
    GTGGAAGAGG AACTGAAGAA GGATCATGCT GAGATGCAGG CAGTTATTGA TGCAAAGCAA
    AAAATAATCG ATGCACAGGT CATAAATGGA GATGAGATTA TGCAAACAGG AAAAACGGAT
    CGTGTCGCTG GATTCGGCCA ACACCAGACT GATGAGTGCG CTGACCCAGG TGAAGGAACG
    GTACAGCATG CAGGTCCGAA ATGGCATCTC CCCCACCAAC CCCACCAAGC TTTCCATCAC
    GGAGAATGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005194943.1
    CDS299..4570
    Translation

    Target ORF information:

    RefSeq Version XM_005194886.3
    Organism Bos taurus(cattle)
    Definition Bos taurus RAS protein activator like 2 (RASAL2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005194886.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAGTCCT GGAAGATGAG CATCTTTAAG AGACAGACAT CACCGTGTCC TCCAAGCTAT 
    CGAAGATCTT TGTCAGAACC TGCTTATACA CACTCAGAGA TAGGAACAAG CTTAAAGAGT
    TATCCATGGC GTGGTCCAGT GAAAAGCAAG AGGAAGAAAA ATGAACAGGC TGTTTCCAGT
    CTAGGAAAGC CACTGGGTGC TTATCGGTGG CAAACACACC ACAGCTTAAG GTTGAAAGGG
    GACAAAAAGG AGCAATTGGA AAATAATAAA GGAGACTTGG ATGAACCACC ACAACAAGCA
    GTCCTGCCAA AGATGGTTGG GGATAATATT TCTTCCTCTG GCAATGAAGT CCTTGGGACA
    TCAATGGAGA AGCAGAACCT GGTAAACACT CAGTCACTTC GGGTTCCATT CAGCAGATCT
    CTTTCAGAGC CAGAACCCGG CTCCAGTGTC AGATTTGTTA GACGGTGGCC AACTTCAGTG
    GGGTCAGTGG AGTCTGATCA GCAGGGATAT TTCATCCGTG GCATTTTCTC TACTCTTTCC
    AATGGCTTCT CCAGGATGCT GAGTCTAAAA GGAGGGTCAA CCAGTGAGCC AGTAAGAGAA
    GATGTGAAAG GGCCACCTAC CCACCGTCTG TCTTGTGGCC AGTCGCCCTA CACCGACACA
    ACAACATGGG AGCGGAAGTA CTGCATCCTC ACAGACAGCC AGCTGGTGTT GCTCAACAAG
    GAGAAGGAGA TACCTGTGGA CGGAGGACAA GAACAGCAGG CGGATTCCAC CAAAGGGCGA
    TGCCTACGGA GAACTGTCAG TGTTCCTTCC GAGGGTCAGT TTCCTGAATA CCCACCAGAG
    GGCGCCACTA AACTGGAGGT GCCAGCAGAA AGGTCCCCAC GCAGACGGAG TATCTCAGGG
    ACCACTACAT CTGAGAAACC CAACTCTATG GACACTGCGA ATACCTCACC CTTCAAAGTA
    CCAGGGTTCT TCAGCAAGCG CCTAAAAGGC TCCATCAAGA GGACCAAAAG CCAGTCAAAG
    CTTGACCGAA ACACGAGCTT TCGGCTTCCC TCTCTTCGCA ATACCGATGA CAGGTCTCGT
    GGGCTGCCTA AACTAAAAGA GTCCCGTTCT CATGAATCTT TGCTGAGCCC ATGCAGTGCC
    GTGGAATGTC TGGATCTCGG TAGAGGGGAG CCTGTGTCGG TAAAGCCACT CCATAGCAGC
    ATCCTTGGAC AAGACTTCTG CTTTGAGGTT ACCTACTTAA GTGGAAGTAA ATGCTTCAGT
    TGTAACTCTG CTTCAGAGAG AGATAAGTGG ATGGAAAACC TGCGCAGGAC AGTTCAACCA
    AATAAGGACA ATTGTAGACG AGCTGAAAAT GTTCTCCGTT TATGGATTAT TGAAGCCAAG
    GACCTTGCCC CTAAAAAGAA ATATTTCTGT GAACTGTGCC TTGATGATAC CCTCTTTGCT
    CGTACAACTA GCAAGACCAA AGCAGACAAT ATTTTCTGGG GTGAACATTT TGAGTTCTAC
    AGCCTTCCAC CTCTTCATAG CATCACAGTT CACATTTATA AAGATGTGGA AAAAAAGAAG
    AAAAAAGACA AGAATAATTA TGTAGGGCTG GTCAACATCC CCACTGCCAG TGTGACTGGT
    CGCCAGTTTG TAGAAAAGTG GTACCCAGTG AGCACACCTA CACCCAACAA AGGAAAGACA
    GGAGGACCTT CTATTCGGAT TAAGTCGCGT TTCCAAACCA TCACCATTCT GCCTATGGAG
    CAATACAAAG AGTTTGCAGA ATTTGTCACC AGCAACTATA CCATGTTATG TTCTGTTCTT
    GAACCAGTAA TCAGTGTGAG AAATAAAGAA GAGCTAGCCT GTGCCTTAGT GCACATTCTT
    CAAAGCACTG GAAGGGCCAA GGATTTTCTG ACGGACTTGG TGATGTCTGA GGTGGATCGT
    TGTGGAGAAC ATGATGTCTT GATCTTCAGA GAGAACACCA TTGCCACCAA ATCCATTGAG
    GAATACCTCA AGTTGGTGGG ACAACAGTAT CTTCACGATG CACTGGGGGA GTTCATCAAA
    GCGTTGTATG AGTCAGATGA AAACTGTGAA GTGGATCCCA GCAAATGTTC ATCCAGTGAA
    CTTCTGGACC ATCAGAGCAA CCTGAAAATG TGCTGTGAGC TGGCTTTCTG CAAGATCATC
    AACTCTTACT GTGTTTTTCC TCGTGAGCTG AAAGAAGTGT TTGCATCCTG GAAGCAGCAG
    TGCCTGAACC GCGGCAAGCA GGACATCAGC GAGAGGCTCA TCAGCGCCTC GCTGTTTCTC
    CGTTTTCTGT GCCCGGCCAT CATGTCTCCC AGTCTCTTCA ACCTCATGCA GGAGTATCCT
    GATGACCGCA CATCTCGGAC TCTGACTCTC ATTGCCAAGG TCATCCAGAA CCTGGCCAAC
    TTTGCCAAAT TTGGTAACAA GGAGGAATAC ATGGCATTCA TGAATGATTT TTTAGAACAT
    GAATGGGGTG GAATGAAGCG CTTTCTTTTG GAGATCTCTA ATCCAGACAC CATCTCAAAC
    ACCCCAGGCT TTGATGGTTA CATTGATCTG GGCCGAGAGC TTTCAGTTTT GCATTCCTTA
    CTATGGGAAG TAGTTTCCCA ACTTGATAAG GGTGAAAATT CCTTCCTACA GGCAACCGTG
    GCAAAATTGG GACCTCTCCC TCGTGTTCTT GCTGATATTA CCAAATCTCT GACTAATCCT
    ACACCAATAC AACAGCAACT GAGACGCTTC ACTGAGCATA ACTCCAGTCC AAATGTCAGT
    GGAAGCCTCT CCTCTGGGCT GCAGAAAATA TTTGAAGACC CTACTGACAG TGATTTGCAC
    AAACTAAAAT CCCCAAGCCA GGACAACACA GACAGTTATT TCAGAGGGAA GACATTATTG
    CTGGTCCAGC AAGCCTCCTC CCAGAGCATG ACTTACTCGG AAAAGGATGA AAAGGAAAGT
    AGCCTCCCCA ATGGCCGCAG CATCTCCCTC ATGGACCTTC AGGAGACGCA CGCAGCCCAA
    GCAGAGCATC CATCTGTGAT ACTCGACGTG CCCATGCGCT TGACCGGAAG CCAGCTCTCC
    ATAACTCAGG TGGCCAGCAT CAAGCAACTG CGGGAGACCC AGAGCACCCC CCAGAGTGCA
    CCCCAAGTGC GAAGGCCCCT GCACCCAGCC TTGAACCAGC CAGGCAGCCT CCAGCCCCTG
    TCCTTCCAGA ACCCTGTCTA TCACCTCAGT AACCCCATTC CTGCAATGCC AAAGGCCTCT
    GTGGACTCCA GTTTGGAGAA CCTAAGCACT GCCAGTTCCA GGAGCCAAAG TAACAGTGAA
    GACTTCAAAC TCAGCGGACC CAGCAACAGC AGCATGGAGG ACTTCACCAA ACGGAGCACG
    CAGAGTGAGG ACTTCTCCAG GCGCCACACC ATGCCCGACA GACACATACC TCTCGCCCTG
    CCGCGGCAGA ACAGCACGGG GCAGACCCAG CTCCGGAAGA TGGACCCGGC GGGGCTGGGC
    GCCCGAGCCA AAGCCCCGCC GTCCCTGCCG CACAGCGCCT CCCTGCGGAG CACCGGGAGC
    ATGTCGGTGG CATCAGCGGC TCTGGTGGCC GAGCCCCTGC AGAACGGGAG CCGGTCCCGA
    CAGCAGTCCT CTTCCTCCAG AGAGAGCCCG GTCCCCAAAG TGAGAGCCAT CCAGAGGCAG
    CAGACCCAGC AGGTTCAGTC ACCTGTGGAC TCCGCCACAA TGTCCCCAGT CGAGAGGACA
    GCAGCCTGGG TCCTGAACAA TGGGCAGTAT GAAGAGGACG TGGAAGAGAC TGAGCAAAAT
    CAGGACGAAG CCAAGCATGC TGAGAAGTAC GAACAGGAAA TCACCAAACT GAAGGAGCGC
    CTGCGCGTGT CCAGCCGGCG ACTCGAGGAG TATGAGCGCC GGTTGCTAGT GCAGGAGCAG
    CAGATGCAGA AGCTGCTGCT GGAGTACAAG GCCCGGCTGG AGGACAGCGA GGAGCGGCTG
    CGCAGGCAGC AGGAGGAGAA GGACAGCCAG ATGAAGAGCA TCATCAGCAG GCTAATGGCT
    GTGGAAGAGG AACTGAAGAA GGATCATGCT GAGATGCAGG CAGTTATTGA TGCAAAGCAA
    AAAATAATCG ATGCACAGGT CATAAATGGA GATGAGATTA TGCAAACAGG AAAAACGGAT
    CGTGTCGCTG GATTCGGCCA ACACCAGACT GATGAGTGCG CTGACCCAGG TGAAGGAACG
    GTACAGCATG CAGGTCCGAA ATGGCATCTC CCCCACCAAC CCCACCAAGC TTTCCATCAC
    GGAGAATGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27405
    Clone ID Related Accession (Same CDS sequence) XM_005194887.3 , XM_005217239.3
    Accession Version XM_005194887.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4263bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ras GTPase-activating protein nGAP isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646324.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005194887.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAGTCCT GGAAGATGAG CATCTTTAAG AGACAGACAT CACCGTGTCC TCCAAGCTAT 
    CGAAGATCTT TGTCAGAACC TGCTTATACA CACTCAGAGA TAGGAACAAG CTTAAAGAGT
    TATCCATGGC GTGGTCCAGT GAAAAGCAAG AGGAAGAAAA ATGAACAGGC TGTTTCCAGT
    CTAGGAAAGC CACTGGGTGC TTATCGGTGG CAAACACACC ACAGCTTAAG GTTGAAAGGG
    GACAAAAAGG AGCAATTGGA AAATAATAAA GGAGACTTGG ATGAACCACC ACAACAAGCA
    GTCCTGCCAA AGATGGTTGG GGATAATATT TCTTCCTCTG GCAATGAAGT CCTTGGGACA
    TCAATGGAGA AGCAGAACCT GGTAAACACT CAGTCACTTC GGGTTCCATT CAGCAGATCT
    CTTTCAGAGC CAGAACCCGG CTCCAGTGTC AGATTTGTTA GACGGTGGCC AACTTCAGTG
    GGGTCAGTGG AGTCTGATCA GCAGGGATAT TTCATCCGTG GCATTTTCTC TACTCTTTCC
    AATGGCTTCT CCAGGATGCT GAGTCTAAAA GGAGGGTCAA CCAGTGAGCC AGTAAGAGAA
    GATGTGAAAG GGCCACCTAC CCACCGTCTG TCTTGTGGCC AGTCGCCCTA CACCGACACA
    ACAACATGGG AGCGGAAGTA CTGCATCCTC ACAGACAGCC AGCTGGTGTT GCTCAACAAG
    GAGAAGGAGA TACCTGTGGA CGGAGGACAA GAACAGCAGG CGGATTCCAC CAAAGGGCGA
    TGCCTACGGA GAACTGTCAG TGTTCCTTCC GAGGGTCAGT TTCCTGAATA CCCACCAGAG
    GGCGCCACTA AACTGGAGGT GCCAGCAGAA AGGTCCCCAC GCAGACGGAG TATCTCAGGG
    ACCACTACAT CTGAGAAACC CAACTCTATG GACACTGCGA ATACCTCACC CTTCAAAGTA
    CCAGGGTTCT TCAGCAAGCG CCTAAAAGGC TCCATCAAGA GGACCAAAAG CCAGTCAAAG
    CTTGACCGAA ACACGAGCTT TCGGCTTCCC TCTCTTCGCA ATACCGATGA CAGGTCTCGT
    GGGCTGCCTA AACTAAAAGA GTCCCGTTCT CATGAATCTT TGCTGAGCCC ATGCAGTGCC
    GTGGAATGTC TGGATCTCGG TAGAGGGGAG CCTGTGTCGG TAAAGCCACT CCATAGCAGC
    ATCCTTGGAC AAGACTTCTG CTTTGAGGTT ACCTACTTAA GTGGAAGTAA ATGCTTCAGT
    TGTAACTCTG CTTCAGAGAG AGATAAGTGG ATGGAAAACC TGCGCAGGAC AGTTCAACCA
    AATAAGGACA ATTGTAGACG AGCTGAAAAT GTTCTCCGTT TATGGATTAT TGAAGCCAAG
    GACCTTGCCC CTAAAAAGAA ATATTTCTGT GAACTGTGCC TTGATGATAC CCTCTTTGCT
    CGTACAACTA GCAAGACCAA AGCAGACAAT ATTTTCTGGG GTGAACATTT TGAGTTCTAC
    AGCCTTCCAC CTCTTCATAG CATCACAGTT CACATTTATA AAGATGTGGA AAAAAAGAAG
    AAAAAAGACA AGAATAATTA TGTAGGGCTG GTCAACATCC CCACTGCCAG TGTGACTGGT
    CGCCAGTTTG TAGAAAAGTG GTACCCAGTG AGCACACCTA CACCCAACAA AGGAAAGACA
    GGAGGACCTT CTATTCGGAT TAAGTCGCGT TTCCAAACCA TCACCATTCT GCCTATGGAG
    CAATACAAAG AGTTTGCAGA ATTTGTCACC AGCAACTATA CCATGTTATG TTCTGTTCTT
    GAACCAGTAA TCAGTGTGAG AAATAAAGAA GAGCTAGCCT GTGCCTTAGT GCACATTCTT
    CAAAGCACTG GAAGGGCCAA GGATTTTCTG ACGGACTTGG TGATGTCTGA GGTGGATCGT
    TGTGGAGAAC ATGATGTCTT GATCTTCAGA GAGAACACCA TTGCCACCAA ATCCATTGAG
    GAATACCTCA AGTTGGTGGG ACAACAGTAT CTTCACGATG CACTGGGGGA GTTCATCAAA
    GCGTTGTATG AGTCAGATGA AAACTGTGAA GTGGATCCCA GCAAATGTTC ATCCAGTGAA
    CTTCTGGACC ATCAGAGCAA CCTGAAAATG TGCTGTGAGC TGGCTTTCTG CAAGATCATC
    AACTCTTACT GTGTTTTTCC TCGTGAGCTG AAAGAAGTGT TTGCATCCTG GAAGCAGCAG
    TGCCTGAACC GCGGCAAGCA GGACATCAGC GAGAGGCTCA TCAGCGCCTC GCTGTTTCTC
    CGTTTTCTGT GCCCGGCCAT CATGTCTCCC AGTCTCTTCA ACCTCATGCA GGAGTATCCT
    GATGACCGCA CATCTCGGAC TCTGACTCTC ATTGCCAAGG TCATCCAGAA CCTGGCCAAC
    TTTGCCAAAT TTGGTAACAA GGAGGAATAC ATGGCATTCA TGAATGATTT TTTAGAACAT
    GAATGGGGTG GAATGAAGCG CTTTCTTTTG GAGATCTCTA ATCCAGACAC CATCTCAAAC
    ACCCCAGGCT TTGATGGTTA CATTGATCTG GGCCGAGAGC TTTCAGTTTT GCATTCCTTA
    CTATGGGAAG TAGTTTCCCA ACTTGATAAG GGTGAAAATT CCTTCCTACA GGCAACCGTG
    GCAAAATTGG GACCTCTCCC TCGTGTTCTT GCTGATATTA CCAAATCTCT GACTAATCCT
    ACACCAATAC AACAGCAACT GAGACGCTTC ACTGAGCATA ACTCCAGTCC AAATGTCAGT
    GGAAGCCTCT CCTCTGGGCT GCAGAAAATA TTTGAAGACC CTACTGACAG TGATTTGCAC
    AAACTAAAAT CCCCAAGCCA GGACAACACA GACAGTTATT TCAGAGGGAA GACATTATTG
    CTGGTCCAGC AAGCCTCCTC CCAGAGCATG ACTTACTCGG AAAAGGATGA AAAGGAAAGT
    AGCCTCCCCA ATGGCCGCAG CATCTCCCTC ATGGACCTTC AGGAGACGCA CGCAGCCCAA
    GCAGAGCATC CATCTGTGAT ACTCGACGTG CCCATGCGCT TGACCGGAAG CCAGCTCTCC
    ATAACTCAGG TGGCCAGCAT CAAGCAACTG CGGGAGACCC AGAGCACCCC CCAGAGTGCA
    CCCCAAGTGC GAAGGCCCCT GCACCCAGCC TTGAACCAGC CAGGCAGCCT CCAGCCCCTG
    TCCTTCCAGA ACCCTGTCTA TCACCTCAGT AACCCCATTC CTGCAATGCC AAAGGCCTCT
    GTGGACTCCA GTTTGGAGAA CCTAAGCACT GCCAGTTCCA GGAGCCAAAG TAACAGTGAA
    GACTTCAAAC TCAGCGGACC CAGCAACAGC AGCATGGAGG ACTTCACCAA ACGGAGCACG
    CAGAGTGAGG ACTTCTCCAG GCGCCACACC ATGCCCGACA GACACATACC TCTCGCCCTG
    CCGCGGCAGA ACAGCACGGG GCAGACCCAG CTCCGGAAGA TGGACCCGGC GGGGCTGGGC
    GCCCGAGCCA AAGCCCCGCC GTCCCTGCCG CACAGCGCCT CCCTGCGGAG CACCGGGAGC
    ATGTCGGTGG CATCAGCGGC TCTGGTGGCC GAGCCCCTGC AGAACGGGAG CCGGTCCCGA
    CAGCAGTCCT CTTCCTCCAG AGAGAGCCCG GTCCCCAAAG TGAGAGCCAT CCAGAGGCAG
    CAGACCCAGC AGGTTCAGTC ACCTGTGGAC TCCGCCACAA TGTCCCCAGT CGAGAGGACA
    GCAGCCTGGG TCCTGAACAA TGGGCAGTAT GAAGAGGACG TGGAAGAGAC TGAGCAAAAT
    CAGGACGAAG CCAAGCATGC TGAGAAGTAC GAACAGGAAA TCACCAAACT GAAGGAGCGC
    CTGCGCGTGT CCAGCCGGCG ACTCGAGGAG TATGAGCGCC GGTTGCTAGT GCAGGAGCAG
    CAGATGCAGA AGCTGCTGCT GGAGTACAAG GCCCGGCTGG AGGACAGCGA GGAGCGGCTG
    CGCAGGCAGC AGGAGGAGAA GGACAGCCAG ATGAAGAGCA TCATCAGCAG GCTAATGGCT
    GTGGAAGAGG AACTGAAGAA GGATCATGCT GAGATGCAGG CAGTTATTGA TGCAAAGCAA
    AAAATAATCG ATGCACAGGA AAAACGGATC GTGTCGCTGG ATTCGGCCAA CACCAGACTG
    ATGAGTGCGC TGACCCAGGT GAAGGAACGG TACAGCATGC AGGTCCGAAA TGGCATCTCC
    CCCACCAACC CCACCAAGCT TTCCATCACG GAGAATGGTG AATTCAAAAA CAGCAGCTGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005194944.1
    CDS296..4558
    Translation

    Target ORF information:

    RefSeq Version XM_005194887.3
    Organism Bos taurus(cattle)
    Definition Bos taurus RAS protein activator like 2 (RASAL2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005194887.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAGTCCT GGAAGATGAG CATCTTTAAG AGACAGACAT CACCGTGTCC TCCAAGCTAT 
    CGAAGATCTT TGTCAGAACC TGCTTATACA CACTCAGAGA TAGGAACAAG CTTAAAGAGT
    TATCCATGGC GTGGTCCAGT GAAAAGCAAG AGGAAGAAAA ATGAACAGGC TGTTTCCAGT
    CTAGGAAAGC CACTGGGTGC TTATCGGTGG CAAACACACC ACAGCTTAAG GTTGAAAGGG
    GACAAAAAGG AGCAATTGGA AAATAATAAA GGAGACTTGG ATGAACCACC ACAACAAGCA
    GTCCTGCCAA AGATGGTTGG GGATAATATT TCTTCCTCTG GCAATGAAGT CCTTGGGACA
    TCAATGGAGA AGCAGAACCT GGTAAACACT CAGTCACTTC GGGTTCCATT CAGCAGATCT
    CTTTCAGAGC CAGAACCCGG CTCCAGTGTC AGATTTGTTA GACGGTGGCC AACTTCAGTG
    GGGTCAGTGG AGTCTGATCA GCAGGGATAT TTCATCCGTG GCATTTTCTC TACTCTTTCC
    AATGGCTTCT CCAGGATGCT GAGTCTAAAA GGAGGGTCAA CCAGTGAGCC AGTAAGAGAA
    GATGTGAAAG GGCCACCTAC CCACCGTCTG TCTTGTGGCC AGTCGCCCTA CACCGACACA
    ACAACATGGG AGCGGAAGTA CTGCATCCTC ACAGACAGCC AGCTGGTGTT GCTCAACAAG
    GAGAAGGAGA TACCTGTGGA CGGAGGACAA GAACAGCAGG CGGATTCCAC CAAAGGGCGA
    TGCCTACGGA GAACTGTCAG TGTTCCTTCC GAGGGTCAGT TTCCTGAATA CCCACCAGAG
    GGCGCCACTA AACTGGAGGT GCCAGCAGAA AGGTCCCCAC GCAGACGGAG TATCTCAGGG
    ACCACTACAT CTGAGAAACC CAACTCTATG GACACTGCGA ATACCTCACC CTTCAAAGTA
    CCAGGGTTCT TCAGCAAGCG CCTAAAAGGC TCCATCAAGA GGACCAAAAG CCAGTCAAAG
    CTTGACCGAA ACACGAGCTT TCGGCTTCCC TCTCTTCGCA ATACCGATGA CAGGTCTCGT
    GGGCTGCCTA AACTAAAAGA GTCCCGTTCT CATGAATCTT TGCTGAGCCC ATGCAGTGCC
    GTGGAATGTC TGGATCTCGG TAGAGGGGAG CCTGTGTCGG TAAAGCCACT CCATAGCAGC
    ATCCTTGGAC AAGACTTCTG CTTTGAGGTT ACCTACTTAA GTGGAAGTAA ATGCTTCAGT
    TGTAACTCTG CTTCAGAGAG AGATAAGTGG ATGGAAAACC TGCGCAGGAC AGTTCAACCA
    AATAAGGACA ATTGTAGACG AGCTGAAAAT GTTCTCCGTT TATGGATTAT TGAAGCCAAG
    GACCTTGCCC CTAAAAAGAA ATATTTCTGT GAACTGTGCC TTGATGATAC CCTCTTTGCT
    CGTACAACTA GCAAGACCAA AGCAGACAAT ATTTTCTGGG GTGAACATTT TGAGTTCTAC
    AGCCTTCCAC CTCTTCATAG CATCACAGTT CACATTTATA AAGATGTGGA AAAAAAGAAG
    AAAAAAGACA AGAATAATTA TGTAGGGCTG GTCAACATCC CCACTGCCAG TGTGACTGGT
    CGCCAGTTTG TAGAAAAGTG GTACCCAGTG AGCACACCTA CACCCAACAA AGGAAAGACA
    GGAGGACCTT CTATTCGGAT TAAGTCGCGT TTCCAAACCA TCACCATTCT GCCTATGGAG
    CAATACAAAG AGTTTGCAGA ATTTGTCACC AGCAACTATA CCATGTTATG TTCTGTTCTT
    GAACCAGTAA TCAGTGTGAG AAATAAAGAA GAGCTAGCCT GTGCCTTAGT GCACATTCTT
    CAAAGCACTG GAAGGGCCAA GGATTTTCTG ACGGACTTGG TGATGTCTGA GGTGGATCGT
    TGTGGAGAAC ATGATGTCTT GATCTTCAGA GAGAACACCA TTGCCACCAA ATCCATTGAG
    GAATACCTCA AGTTGGTGGG ACAACAGTAT CTTCACGATG CACTGGGGGA GTTCATCAAA
    GCGTTGTATG AGTCAGATGA AAACTGTGAA GTGGATCCCA GCAAATGTTC ATCCAGTGAA
    CTTCTGGACC ATCAGAGCAA CCTGAAAATG TGCTGTGAGC TGGCTTTCTG CAAGATCATC
    AACTCTTACT GTGTTTTTCC TCGTGAGCTG AAAGAAGTGT TTGCATCCTG GAAGCAGCAG
    TGCCTGAACC GCGGCAAGCA GGACATCAGC GAGAGGCTCA TCAGCGCCTC GCTGTTTCTC
    CGTTTTCTGT GCCCGGCCAT CATGTCTCCC AGTCTCTTCA ACCTCATGCA GGAGTATCCT
    GATGACCGCA CATCTCGGAC TCTGACTCTC ATTGCCAAGG TCATCCAGAA CCTGGCCAAC
    TTTGCCAAAT TTGGTAACAA GGAGGAATAC ATGGCATTCA TGAATGATTT TTTAGAACAT
    GAATGGGGTG GAATGAAGCG CTTTCTTTTG GAGATCTCTA ATCCAGACAC CATCTCAAAC
    ACCCCAGGCT TTGATGGTTA CATTGATCTG GGCCGAGAGC TTTCAGTTTT GCATTCCTTA
    CTATGGGAAG TAGTTTCCCA ACTTGATAAG GGTGAAAATT CCTTCCTACA GGCAACCGTG
    GCAAAATTGG GACCTCTCCC TCGTGTTCTT GCTGATATTA CCAAATCTCT GACTAATCCT
    ACACCAATAC AACAGCAACT GAGACGCTTC ACTGAGCATA ACTCCAGTCC AAATGTCAGT
    GGAAGCCTCT CCTCTGGGCT GCAGAAAATA TTTGAAGACC CTACTGACAG TGATTTGCAC
    AAACTAAAAT CCCCAAGCCA GGACAACACA GACAGTTATT TCAGAGGGAA GACATTATTG
    CTGGTCCAGC AAGCCTCCTC CCAGAGCATG ACTTACTCGG AAAAGGATGA AAAGGAAAGT
    AGCCTCCCCA ATGGCCGCAG CATCTCCCTC ATGGACCTTC AGGAGACGCA CGCAGCCCAA
    GCAGAGCATC CATCTGTGAT ACTCGACGTG CCCATGCGCT TGACCGGAAG CCAGCTCTCC
    ATAACTCAGG TGGCCAGCAT CAAGCAACTG CGGGAGACCC AGAGCACCCC CCAGAGTGCA
    CCCCAAGTGC GAAGGCCCCT GCACCCAGCC TTGAACCAGC CAGGCAGCCT CCAGCCCCTG
    TCCTTCCAGA ACCCTGTCTA TCACCTCAGT AACCCCATTC CTGCAATGCC AAAGGCCTCT
    GTGGACTCCA GTTTGGAGAA CCTAAGCACT GCCAGTTCCA GGAGCCAAAG TAACAGTGAA
    GACTTCAAAC TCAGCGGACC CAGCAACAGC AGCATGGAGG ACTTCACCAA ACGGAGCACG
    CAGAGTGAGG ACTTCTCCAG GCGCCACACC ATGCCCGACA GACACATACC TCTCGCCCTG
    CCGCGGCAGA ACAGCACGGG GCAGACCCAG CTCCGGAAGA TGGACCCGGC GGGGCTGGGC
    GCCCGAGCCA AAGCCCCGCC GTCCCTGCCG CACAGCGCCT CCCTGCGGAG CACCGGGAGC
    ATGTCGGTGG CATCAGCGGC TCTGGTGGCC GAGCCCCTGC AGAACGGGAG CCGGTCCCGA
    CAGCAGTCCT CTTCCTCCAG AGAGAGCCCG GTCCCCAAAG TGAGAGCCAT CCAGAGGCAG
    CAGACCCAGC AGGTTCAGTC ACCTGTGGAC TCCGCCACAA TGTCCCCAGT CGAGAGGACA
    GCAGCCTGGG TCCTGAACAA TGGGCAGTAT GAAGAGGACG TGGAAGAGAC TGAGCAAAAT
    CAGGACGAAG CCAAGCATGC TGAGAAGTAC GAACAGGAAA TCACCAAACT GAAGGAGCGC
    CTGCGCGTGT CCAGCCGGCG ACTCGAGGAG TATGAGCGCC GGTTGCTAGT GCAGGAGCAG
    CAGATGCAGA AGCTGCTGCT GGAGTACAAG GCCCGGCTGG AGGACAGCGA GGAGCGGCTG
    CGCAGGCAGC AGGAGGAGAA GGACAGCCAG ATGAAGAGCA TCATCAGCAG GCTAATGGCT
    GTGGAAGAGG AACTGAAGAA GGATCATGCT GAGATGCAGG CAGTTATTGA TGCAAAGCAA
    AAAATAATCG ATGCACAGGA AAAACGGATC GTGTCGCTGG ATTCGGCCAA CACCAGACTG
    ATGAGTGCGC TGACCCAGGT GAAGGAACGG TACAGCATGC AGGTCCGAAA TGGCATCTCC
    CCCACCAACC CCACCAAGCT TTCCATCACG GAGAATGGTG AATTCAAAAA CAGCAGCTGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27405
    Clone ID Related Accession (Same CDS sequence) XM_005194887.3 , XM_005217239.3
    Accession Version XM_005217239.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4263bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ras GTPase-activating protein nGAP isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104439.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005217239.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAGTCCT GGAAGATGAG CATCTTTAAG AGACAGACAT CACCGTGTCC TCCAAGCTAT 
    CGAAGATCTT TGTCAGAACC TGCTTATACA CACTCAGAGA TAGGAACAAG CTTAAAGAGT
    TATCCATGGC GTGGTCCAGT GAAAAGCAAG AGGAAGAAAA ATGAACAGGC TGTTTCCAGT
    CTAGGAAAGC CACTGGGTGC TTATCGGTGG CAAACACACC ACAGCTTAAG GTTGAAAGGG
    GACAAAAAGG AGCAATTGGA AAATAATAAA GGAGACTTGG ATGAACCACC ACAACAAGCA
    GTCCTGCCAA AGATGGTTGG GGATAATATT TCTTCCTCTG GCAATGAAGT CCTTGGGACA
    TCAATGGAGA AGCAGAACCT GGTAAACACT CAGTCACTTC GGGTTCCATT CAGCAGATCT
    CTTTCAGAGC CAGAACCCGG CTCCAGTGTC AGATTTGTTA GACGGTGGCC AACTTCAGTG
    GGGTCAGTGG AGTCTGATCA GCAGGGATAT TTCATCCGTG GCATTTTCTC TACTCTTTCC
    AATGGCTTCT CCAGGATGCT GAGTCTAAAA GGAGGGTCAA CCAGTGAGCC AGTAAGAGAA
    GATGTGAAAG GGCCACCTAC CCACCGTCTG TCTTGTGGCC AGTCGCCCTA CACCGACACA
    ACAACATGGG AGCGGAAGTA CTGCATCCTC ACAGACAGCC AGCTGGTGTT GCTCAACAAG
    GAGAAGGAGA TACCTGTGGA CGGAGGACAA GAACAGCAGG CGGATTCCAC CAAAGGGCGA
    TGCCTACGGA GAACTGTCAG TGTTCCTTCC GAGGGTCAGT TTCCTGAATA CCCACCAGAG
    GGCGCCACTA AACTGGAGGT GCCAGCAGAA AGGTCCCCAC GCAGACGGAG TATCTCAGGG
    ACCACTACAT CTGAGAAACC CAACTCTATG GACACTGCGA ATACCTCACC CTTCAAAGTA
    CCAGGGTTCT TCAGCAAGCG CCTAAAAGGC TCCATCAAGA GGACCAAAAG CCAGTCAAAG
    CTTGACCGAA ACACGAGCTT TCGGCTTCCC TCTCTTCGCA ATACCGATGA CAGGTCTCGT
    GGGCTGCCTA AACTAAAAGA GTCCCGTTCT CATGAATCTT TGCTGAGCCC ATGCAGTGCC
    GTGGAATGTC TGGATCTCGG TAGAGGGGAG CCTGTGTCGG TAAAGCCACT CCATAGCAGC
    ATCCTTGGAC AAGACTTCTG CTTTGAGGTT ACCTACTTAA GTGGAAGTAA ATGCTTCAGT
    TGTAACTCTG CTTCAGAGAG AGATAAGTGG ATGGAAAACC TGCGCAGGAC AGTTCAACCA
    AATAAGGACA ATTGTAGACG AGCTGAAAAT GTTCTCCGTT TATGGATTAT TGAAGCCAAG
    GACCTTGCCC CTAAAAAGAA ATATTTCTGT GAACTGTGCC TTGATGATAC CCTCTTTGCT
    CGTACAACTA GCAAGACCAA AGCAGACAAT ATTTTCTGGG GTGAACATTT TGAGTTCTAC
    AGCCTTCCAC CTCTTCATAG CATCACAGTT CACATTTATA AAGATGTGGA AAAAAAGAAG
    AAAAAAGACA AGAATAATTA TGTAGGGCTG GTCAACATCC CCACTGCCAG TGTGACTGGT
    CGCCAGTTTG TAGAAAAGTG GTACCCAGTG AGCACACCTA CACCCAACAA AGGAAAGACA
    GGAGGACCTT CTATTCGGAT TAAGTCGCGT TTCCAAACCA TCACCATTCT GCCTATGGAG
    CAATACAAAG AGTTTGCAGA ATTTGTCACC AGCAACTATA CCATGTTATG TTCTGTTCTT
    GAACCAGTAA TCAGTGTGAG AAATAAAGAA GAGCTAGCCT GTGCCTTAGT GCACATTCTT
    CAAAGCACTG GAAGGGCCAA GGATTTTCTG ACGGACTTGG TGATGTCTGA GGTGGATCGT
    TGTGGAGAAC ATGATGTCTT GATCTTCAGA GAGAACACCA TTGCCACCAA ATCCATTGAG
    GAATACCTCA AGTTGGTGGG ACAACAGTAT CTTCACGATG CACTGGGGGA GTTCATCAAA
    GCGTTGTATG AGTCAGATGA AAACTGTGAA GTGGATCCCA GCAAATGTTC ATCCAGTGAA
    CTTCTGGACC ATCAGAGCAA CCTGAAAATG TGCTGTGAGC TGGCTTTCTG CAAGATCATC
    AACTCTTACT GTGTTTTTCC TCGTGAGCTG AAAGAAGTGT TTGCATCCTG GAAGCAGCAG
    TGCCTGAACC GCGGCAAGCA GGACATCAGC GAGAGGCTCA TCAGCGCCTC GCTGTTTCTC
    CGTTTTCTGT GCCCGGCCAT CATGTCTCCC AGTCTCTTCA ACCTCATGCA GGAGTATCCT
    GATGACCGCA CATCTCGGAC TCTGACTCTC ATTGCCAAGG TCATCCAGAA CCTGGCCAAC
    TTTGCCAAAT TTGGTAACAA GGAGGAATAC ATGGCATTCA TGAATGATTT TTTAGAACAT
    GAATGGGGTG GAATGAAGCG CTTTCTTTTG GAGATCTCTA ATCCAGACAC CATCTCAAAC
    ACCCCAGGCT TTGATGGTTA CATTGATCTG GGCCGAGAGC TTTCAGTTTT GCATTCCTTA
    CTATGGGAAG TAGTTTCCCA ACTTGATAAG GGTGAAAATT CCTTCCTACA GGCAACCGTG
    GCAAAATTGG GACCTCTCCC TCGTGTTCTT GCTGATATTA CCAAATCTCT GACTAATCCT
    ACACCAATAC AACAGCAACT GAGACGCTTC ACTGAGCATA ACTCCAGTCC AAATGTCAGT
    GGAAGCCTCT CCTCTGGGCT GCAGAAAATA TTTGAAGACC CTACTGACAG TGATTTGCAC
    AAACTAAAAT CCCCAAGCCA GGACAACACA GACAGTTATT TCAGAGGGAA GACATTATTG
    CTGGTCCAGC AAGCCTCCTC CCAGAGCATG ACTTACTCGG AAAAGGATGA AAAGGAAAGT
    AGCCTCCCCA ATGGCCGCAG CATCTCCCTC ATGGACCTTC AGGAGACGCA CGCAGCCCAA
    GCAGAGCATC CATCTGTGAT ACTCGACGTG CCCATGCGCT TGACCGGAAG CCAGCTCTCC
    ATAACTCAGG TGGCCAGCAT CAAGCAACTG CGGGAGACCC AGAGCACCCC CCAGAGTGCA
    CCCCAAGTGC GAAGGCCCCT GCACCCAGCC TTGAACCAGC CAGGCAGCCT CCAGCCCCTG
    TCCTTCCAGA ACCCTGTCTA TCACCTCAGT AACCCCATTC CTGCAATGCC AAAGGCCTCT
    GTGGACTCCA GTTTGGAGAA CCTAAGCACT GCCAGTTCCA GGAGCCAAAG TAACAGTGAA
    GACTTCAAAC TCAGCGGACC CAGCAACAGC AGCATGGAGG ACTTCACCAA ACGGAGCACG
    CAGAGTGAGG ACTTCTCCAG GCGCCACACC ATGCCCGACA GACACATACC TCTCGCCCTG
    CCGCGGCAGA ACAGCACGGG GCAGACCCAG CTCCGGAAGA TGGACCCGGC GGGGCTGGGC
    GCCCGAGCCA AAGCCCCGCC GTCCCTGCCG CACAGCGCCT CCCTGCGGAG CACCGGGAGC
    ATGTCGGTGG CATCAGCGGC TCTGGTGGCC GAGCCCCTGC AGAACGGGAG CCGGTCCCGA
    CAGCAGTCCT CTTCCTCCAG AGAGAGCCCG GTCCCCAAAG TGAGAGCCAT CCAGAGGCAG
    CAGACCCAGC AGGTTCAGTC ACCTGTGGAC TCCGCCACAA TGTCCCCAGT CGAGAGGACA
    GCAGCCTGGG TCCTGAACAA TGGGCAGTAT GAAGAGGACG TGGAAGAGAC TGAGCAAAAT
    CAGGACGAAG CCAAGCATGC TGAGAAGTAC GAACAGGAAA TCACCAAACT GAAGGAGCGC
    CTGCGCGTGT CCAGCCGGCG ACTCGAGGAG TATGAGCGCC GGTTGCTAGT GCAGGAGCAG
    CAGATGCAGA AGCTGCTGCT GGAGTACAAG GCCCGGCTGG AGGACAGCGA GGAGCGGCTG
    CGCAGGCAGC AGGAGGAGAA GGACAGCCAG ATGAAGAGCA TCATCAGCAG GCTAATGGCT
    GTGGAAGAGG AACTGAAGAA GGATCATGCT GAGATGCAGG CAGTTATTGA TGCAAAGCAA
    AAAATAATCG ATGCACAGGA AAAACGGATC GTGTCGCTGG ATTCGGCCAA CACCAGACTG
    ATGAGTGCGC TGACCCAGGT GAAGGAACGG TACAGCATGC AGGTCCGAAA TGGCATCTCC
    CCCACCAACC CCACCAAGCT TTCCATCACG GAGAATGGTG AATTCAAAAA CAGCAGCTGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005217296.1
    CDS296..4558
    Translation

    Target ORF information:

    RefSeq Version XM_005217239.3
    Organism Bos taurus(cattle)
    Definition Bos taurus RAS protein activator like 2 (RASAL2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005217239.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAGTCCT GGAAGATGAG CATCTTTAAG AGACAGACAT CACCGTGTCC TCCAAGCTAT 
    CGAAGATCTT TGTCAGAACC TGCTTATACA CACTCAGAGA TAGGAACAAG CTTAAAGAGT
    TATCCATGGC GTGGTCCAGT GAAAAGCAAG AGGAAGAAAA ATGAACAGGC TGTTTCCAGT
    CTAGGAAAGC CACTGGGTGC TTATCGGTGG CAAACACACC ACAGCTTAAG GTTGAAAGGG
    GACAAAAAGG AGCAATTGGA AAATAATAAA GGAGACTTGG ATGAACCACC ACAACAAGCA
    GTCCTGCCAA AGATGGTTGG GGATAATATT TCTTCCTCTG GCAATGAAGT CCTTGGGACA
    TCAATGGAGA AGCAGAACCT GGTAAACACT CAGTCACTTC GGGTTCCATT CAGCAGATCT
    CTTTCAGAGC CAGAACCCGG CTCCAGTGTC AGATTTGTTA GACGGTGGCC AACTTCAGTG
    GGGTCAGTGG AGTCTGATCA GCAGGGATAT TTCATCCGTG GCATTTTCTC TACTCTTTCC
    AATGGCTTCT CCAGGATGCT GAGTCTAAAA GGAGGGTCAA CCAGTGAGCC AGTAAGAGAA
    GATGTGAAAG GGCCACCTAC CCACCGTCTG TCTTGTGGCC AGTCGCCCTA CACCGACACA
    ACAACATGGG AGCGGAAGTA CTGCATCCTC ACAGACAGCC AGCTGGTGTT GCTCAACAAG
    GAGAAGGAGA TACCTGTGGA CGGAGGACAA GAACAGCAGG CGGATTCCAC CAAAGGGCGA
    TGCCTACGGA GAACTGTCAG TGTTCCTTCC GAGGGTCAGT TTCCTGAATA CCCACCAGAG
    GGCGCCACTA AACTGGAGGT GCCAGCAGAA AGGTCCCCAC GCAGACGGAG TATCTCAGGG
    ACCACTACAT CTGAGAAACC CAACTCTATG GACACTGCGA ATACCTCACC CTTCAAAGTA
    CCAGGGTTCT TCAGCAAGCG CCTAAAAGGC TCCATCAAGA GGACCAAAAG CCAGTCAAAG
    CTTGACCGAA ACACGAGCTT TCGGCTTCCC TCTCTTCGCA ATACCGATGA CAGGTCTCGT
    GGGCTGCCTA AACTAAAAGA GTCCCGTTCT CATGAATCTT TGCTGAGCCC ATGCAGTGCC
    GTGGAATGTC TGGATCTCGG TAGAGGGGAG CCTGTGTCGG TAAAGCCACT CCATAGCAGC
    ATCCTTGGAC AAGACTTCTG CTTTGAGGTT ACCTACTTAA GTGGAAGTAA ATGCTTCAGT
    TGTAACTCTG CTTCAGAGAG AGATAAGTGG ATGGAAAACC TGCGCAGGAC AGTTCAACCA
    AATAAGGACA ATTGTAGACG AGCTGAAAAT GTTCTCCGTT TATGGATTAT TGAAGCCAAG
    GACCTTGCCC CTAAAAAGAA ATATTTCTGT GAACTGTGCC TTGATGATAC CCTCTTTGCT
    CGTACAACTA GCAAGACCAA AGCAGACAAT ATTTTCTGGG GTGAACATTT TGAGTTCTAC
    AGCCTTCCAC CTCTTCATAG CATCACAGTT CACATTTATA AAGATGTGGA AAAAAAGAAG
    AAAAAAGACA AGAATAATTA TGTAGGGCTG GTCAACATCC CCACTGCCAG TGTGACTGGT
    CGCCAGTTTG TAGAAAAGTG GTACCCAGTG AGCACACCTA CACCCAACAA AGGAAAGACA
    GGAGGACCTT CTATTCGGAT TAAGTCGCGT TTCCAAACCA TCACCATTCT GCCTATGGAG
    CAATACAAAG AGTTTGCAGA ATTTGTCACC AGCAACTATA CCATGTTATG TTCTGTTCTT
    GAACCAGTAA TCAGTGTGAG AAATAAAGAA GAGCTAGCCT GTGCCTTAGT GCACATTCTT
    CAAAGCACTG GAAGGGCCAA GGATTTTCTG ACGGACTTGG TGATGTCTGA GGTGGATCGT
    TGTGGAGAAC ATGATGTCTT GATCTTCAGA GAGAACACCA TTGCCACCAA ATCCATTGAG
    GAATACCTCA AGTTGGTGGG ACAACAGTAT CTTCACGATG CACTGGGGGA GTTCATCAAA
    GCGTTGTATG AGTCAGATGA AAACTGTGAA GTGGATCCCA GCAAATGTTC ATCCAGTGAA
    CTTCTGGACC ATCAGAGCAA CCTGAAAATG TGCTGTGAGC TGGCTTTCTG CAAGATCATC
    AACTCTTACT GTGTTTTTCC TCGTGAGCTG AAAGAAGTGT TTGCATCCTG GAAGCAGCAG
    TGCCTGAACC GCGGCAAGCA GGACATCAGC GAGAGGCTCA TCAGCGCCTC GCTGTTTCTC
    CGTTTTCTGT GCCCGGCCAT CATGTCTCCC AGTCTCTTCA ACCTCATGCA GGAGTATCCT
    GATGACCGCA CATCTCGGAC TCTGACTCTC ATTGCCAAGG TCATCCAGAA CCTGGCCAAC
    TTTGCCAAAT TTGGTAACAA GGAGGAATAC ATGGCATTCA TGAATGATTT TTTAGAACAT
    GAATGGGGTG GAATGAAGCG CTTTCTTTTG GAGATCTCTA ATCCAGACAC CATCTCAAAC
    ACCCCAGGCT TTGATGGTTA CATTGATCTG GGCCGAGAGC TTTCAGTTTT GCATTCCTTA
    CTATGGGAAG TAGTTTCCCA ACTTGATAAG GGTGAAAATT CCTTCCTACA GGCAACCGTG
    GCAAAATTGG GACCTCTCCC TCGTGTTCTT GCTGATATTA CCAAATCTCT GACTAATCCT
    ACACCAATAC AACAGCAACT GAGACGCTTC ACTGAGCATA ACTCCAGTCC AAATGTCAGT
    GGAAGCCTCT CCTCTGGGCT GCAGAAAATA TTTGAAGACC CTACTGACAG TGATTTGCAC
    AAACTAAAAT CCCCAAGCCA GGACAACACA GACAGTTATT TCAGAGGGAA GACATTATTG
    CTGGTCCAGC AAGCCTCCTC CCAGAGCATG ACTTACTCGG AAAAGGATGA AAAGGAAAGT
    AGCCTCCCCA ATGGCCGCAG CATCTCCCTC ATGGACCTTC AGGAGACGCA CGCAGCCCAA
    GCAGAGCATC CATCTGTGAT ACTCGACGTG CCCATGCGCT TGACCGGAAG CCAGCTCTCC
    ATAACTCAGG TGGCCAGCAT CAAGCAACTG CGGGAGACCC AGAGCACCCC CCAGAGTGCA
    CCCCAAGTGC GAAGGCCCCT GCACCCAGCC TTGAACCAGC CAGGCAGCCT CCAGCCCCTG
    TCCTTCCAGA ACCCTGTCTA TCACCTCAGT AACCCCATTC CTGCAATGCC AAAGGCCTCT
    GTGGACTCCA GTTTGGAGAA CCTAAGCACT GCCAGTTCCA GGAGCCAAAG TAACAGTGAA
    GACTTCAAAC TCAGCGGACC CAGCAACAGC AGCATGGAGG ACTTCACCAA ACGGAGCACG
    CAGAGTGAGG ACTTCTCCAG GCGCCACACC ATGCCCGACA GACACATACC TCTCGCCCTG
    CCGCGGCAGA ACAGCACGGG GCAGACCCAG CTCCGGAAGA TGGACCCGGC GGGGCTGGGC
    GCCCGAGCCA AAGCCCCGCC GTCCCTGCCG CACAGCGCCT CCCTGCGGAG CACCGGGAGC
    ATGTCGGTGG CATCAGCGGC TCTGGTGGCC GAGCCCCTGC AGAACGGGAG CCGGTCCCGA
    CAGCAGTCCT CTTCCTCCAG AGAGAGCCCG GTCCCCAAAG TGAGAGCCAT CCAGAGGCAG
    CAGACCCAGC AGGTTCAGTC ACCTGTGGAC TCCGCCACAA TGTCCCCAGT CGAGAGGACA
    GCAGCCTGGG TCCTGAACAA TGGGCAGTAT GAAGAGGACG TGGAAGAGAC TGAGCAAAAT
    CAGGACGAAG CCAAGCATGC TGAGAAGTAC GAACAGGAAA TCACCAAACT GAAGGAGCGC
    CTGCGCGTGT CCAGCCGGCG ACTCGAGGAG TATGAGCGCC GGTTGCTAGT GCAGGAGCAG
    CAGATGCAGA AGCTGCTGCT GGAGTACAAG GCCCGGCTGG AGGACAGCGA GGAGCGGCTG
    CGCAGGCAGC AGGAGGAGAA GGACAGCCAG ATGAAGAGCA TCATCAGCAG GCTAATGGCT
    GTGGAAGAGG AACTGAAGAA GGATCATGCT GAGATGCAGG CAGTTATTGA TGCAAAGCAA
    AAAATAATCG ATGCACAGGA AAAACGGATC GTGTCGCTGG ATTCGGCCAA CACCAGACTG
    ATGAGTGCGC TGACCCAGGT GAAGGAACGG TACAGCATGC AGGTCCGAAA TGGCATCTCC
    CCCACCAACC CCACCAAGCT TTCCATCACG GAGAATGGTG AATTCAAAAA CAGCAGCTGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27406
    Clone ID Related Accession (Same CDS sequence) XM_005217240.3 , XM_005194888.3
    Accession Version XM_005217240.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4251bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ras GTPase-activating protein nGAP isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104439.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005217240.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGAGTCCT GGAAGATGAG CATCTTTAAG AGACAGACAT CACCGTGTCC TCCAAGCTAT 
    CGAAGATCTT TGTCAGAACC TGCTTATACA CACTCAGAGA TAGGAACAAG CTTAAAGAGT
    TATCCATGGC GTGGTCCAGT GAAAAGCAAG AGGAAGAAAA ATGAACAGGC TGTTTCCAGT
    CTAGGAAAGC CACTGGGTGC TTATCGGTGG CAAACACACC ACAGCTTAAG GTTGAAAGGG
    GACAAAAAGG AGCAATTGGA AAATAATAAA GGAGACTTGG ATGAACCACC ACAACAAGCA
    GTCCTGCCAA AGATGGTTGG GGATAATATT TCTTCCTCTG GCAATGAAGT CCTTGGGACA
    TCAATGGAGA AGCAGAACCT GGTAAACACT CAGTCACTTC GGGTTCCATT CAGCAGATCT
    CTTTCAGAGC CAGAACCCGG CTCCAGTGTC AGATTTGTTA GACGGTGGCC AACTTCAGTG
    GGGTCAGTGG AGTCTGATCA GCAGGGATAT TTCATCCGTG GCATTTTCTC TACTCTTTCC
    AATGGCTTCT CCAGGATGCT GAGTCTAAAA GGAGGGTCAA CCAGTGAGCC AGTAAGAGAA
    GATGTGAAAG GGCCACCTAC CCACCGTCTG TCTTGTGGCC AGTCGCCCTA CACCGACACA
    ACAACATGGG AGCGGAAGTA CTGCATCCTC ACAGACAGCC AGCTGGTGTT GCTCAACAAG
    GAGAAGGAGA TACCTGTGGA CGGAGGACAA GAACAGCAGG CGGATTCCAC CAAAGGGCGA
    TGCCTACGGA GAACTGTCAG TGTTCCTTCC GAGGGTCAGT TTCCTGAATA CCCACCAGAG
    GGCGCCACTA AACTGGAGGT GCCAGCAGAA AGGTCCCCAC GCAGACGGAG TATCTCAGGG
    ACCACTACAT CTGAGAAACC CAACTCTATG GACACTGCGA ATACCTCACC CTTCAAAGTA
    CCAGGGTTCT TCAGCAAGCG CCTAAAAGGC TCCATCAAGA GGACCAAAAG CCAGTCAAAG
    CTTGACCGAA ACACGAGCTT TCGGCTTCCC TCTCTTCGCA ATACCGATGA CAGGTCTCGT
    GGGCTGCCTA AACTAAAAGA GTCCCGTTCT CATGAATCTT TGCTGAGCCC ATGCAGTGCC
    GTGGAATGTC TGGATCTCGG TAGAGGGGAG CCTGTGTCGG TAAAGCCACT CCATAGCAGC
    ATCCTTGGAC AAGACTTCTG CTTTGAGGTT ACCTACTTAA GTGGAAGTAA ATGCTTCAGT
    TGTAACTCTG CTTCAGAGAG AGATAAGTGG ATGGAAAACC TGCGCAGGAC AGTTCAACCA
    AATAAGGACA ATTGTAGACG AGCTGAAAAT GTTCTCCGTT TATGGATTAT TGAAGCCAAG
    GACCTTGCCC CTAAAAAGAA ATATTTCTGT GAACTGTGCC TTGATGATAC CCTCTTTGCT
    CGTACAACTA GCAAGACCAA AGCAGACAAT ATTTTCTGGG GTGAACATTT TGAGTTCTAC
    AGCCTTCCAC CTCTTCATAG CATCACAGTT CACATTTATA AAGATGTGGA AAAAAAGAAG
    AAAAAAGACA AGAATAATTA TGTAGGGCTG GTCAACATCC CCACTGCCAG TGTGACTGGT
    CGCCAGTTTG TAGAAAAGTG GTACCCAGTG AGCACACCTA CACCCAACAA AGGAAAGACA
    GGAGGACCTT CTATTCGGAT TAAGTCGCGT TTCCAAACCA TCACCATTCT GCCTATGGAG
    CAATACAAAG AGTTTGCAGA ATTTGTCACC AGCAACTATA CCATGTTATG TTCTGTTCTT
    GAACCAGTAA TCAGTGTGAG AAATAAAGAA GAGCTAGCCT GTGCCTTAGT GCACATTCTT
    CAAAGCACTG GAAGGGCCAA GGATTTTCTG ACGGACTTGG TGATGTCTGA GGTGGATCGT
    TGTGGAGAAC ATGATGTCTT GATCTTCAGA GAGAACACCA TTGCCACCAA ATCCATTGAG
    GAATACCTCA AGTTGGTGGG ACAACAGTAT CTTCACGATG CACTGGGGGA GTTCATCAAA
    GCGTTGTATG AGTCAGATGA AAACTGTGAA GTGGATCCCA GCAAATGTTC ATCCAGTGAA
    CTTCTGGACC ATCAGAGCAA CCTGAAAATG TGCTGTGAGC TGGCTTTCTG CAAGATCATC
    AACTCTTACT GTGTTTTTCC TCGTGAGCTG AAAGAAGTGT TTGCATCCTG GAAGCAGCAG
    TGCCTGAACC GCGGCAAGCA GGACATCAGC GAGAGGCTCA TCAGCGCCTC GCTGTTTCTC
    CGTTTTCTGT GCCCGGCCAT CATGTCTCCC AGTCTCTTCA ACCTCATGCA GGAGTATCCT
    GATGACCGCA CATCTCGGAC TCTGACTCTC ATTGCCAAGG TCATCCAGAA CCTGGCCAAC
    TTTGCCAAAT TTGGTAACAA GGAGGAATAC ATGGCATTCA TGAATGATTT TTTAGAACAT
    GAATGGGGTG GAATGAAGCG CTTTCTTTTG GAGATCTCTA ATCCAGACAC CATCTCAAAC
    ACCCCAGGCT TTGATGGTTA CATTGATCTG GGCCGAGAGC TTTCAGTTTT GCATTCCTTA
    CTATGGGAAG TAGTTTCCCA ACTTGATAAG GCAACCGTGG CAAAATTGGG ACCTCTCCCT
    CGTGTTCTTG CTGATATTAC CAAATCTCTG ACTAATCCTA CACCAATACA ACAGCAACTG
    AGACGCTTCA CTGAGCATAA CTCCAGTCCA AATGTCAGTG GAAGCCTCTC CTCTGGGCTG
    CAGAAAATAT TTGAAGACCC TACTGACAGT GATTTGCACA AACTAAAATC CCCAAGCCAG
    GACAACACAG ACAGTTATTT CAGAGGGAAG ACATTATTGC TGGTCCAGCA AGCCTCCTCC
    CAGAGCATGA CTTACTCGGA AAAGGATGAA AAGGAAAGTA GCCTCCCCAA TGGCCGCAGC
    ATCTCCCTCA TGGACCTTCA GGAGACGCAC GCAGCCCAAG CAGAGCATCC ATCTGTGATA
    CTCGACGTGC CCATGCGCTT GACCGGAAGC CAGCTCTCCA TAACTCAGGT GGCCAGCATC
    AAGCAACTGC GGGAGACCCA GAGCACCCCC CAGAGTGCAC CCCAAGTGCG AAGGCCCCTG
    CACCCAGCCT TGAACCAGCC AGGCAGCCTC CAGCCCCTGT CCTTCCAGAA CCCTGTCTAT
    CACCTCAGTA ACCCCATTCC TGCAATGCCA AAGGCCTCTG TGGACTCCAG TTTGGAGAAC
    CTAAGCACTG CCAGTTCCAG GAGCCAAAGT AACAGTGAAG ACTTCAAACT CAGCGGACCC
    AGCAACAGCA GCATGGAGGA CTTCACCAAA CGGAGCACGC AGAGTGAGGA CTTCTCCAGG
    CGCCACACCA TGCCCGACAG ACACATACCT CTCGCCCTGC CGCGGCAGAA CAGCACGGGG
    CAGACCCAGC TCCGGAAGAT GGACCCGGCG GGGCTGGGCG CCCGAGCCAA AGCCCCGCCG
    TCCCTGCCGC ACAGCGCCTC CCTGCGGAGC ACCGGGAGCA TGTCGGTGGC ATCAGCGGCT
    CTGGTGGCCG AGCCCCTGCA GAACGGGAGC CGGTCCCGAC AGCAGTCCTC TTCCTCCAGA
    GAGAGCCCGG TCCCCAAAGT GAGAGCCATC CAGAGGCAGC AGACCCAGCA GGTTCAGTCA
    CCTGTGGACT CCGCCACAAT GTCCCCAGTC GAGAGGACAG CAGCCTGGGT CCTGAACAAT
    GGGCAGTATG AAGAGGACGT GGAAGAGACT GAGCAAAATC AGGACGAAGC CAAGCATGCT
    GAGAAGTACG AACAGGAAAT CACCAAACTG AAGGAGCGCC TGCGCGTGTC CAGCCGGCGA
    CTCGAGGAGT ATGAGCGCCG GTTGCTAGTG CAGGAGCAGC AGATGCAGAA GCTGCTGCTG
    GAGTACAAGG CCCGGCTGGA GGACAGCGAG GAGCGGCTGC GCAGGCAGCA GGAGGAGAAG
    GACAGCCAGA TGAAGAGCAT CATCAGCAGG CTAATGGCTG TGGAAGAGGA ACTGAAGAAG
    GATCATGCTG AGATGCAGGC AGTTATTGAT GCAAAGCAAA AAATAATCGA TGCACAGGTC
    ATAAATGGAG ATGAGATTAT GCAAACAGGA AAAACGGATC GTGTCGCTGG ATTCGGCCAA
    CACCAGACTG ATGAGTGCGC TGACCCAGGT GAAGGAACGG TACAGCATGC AGGTCCGAAA
    TGGCATCTCC CCCACCAACC CCACCAAGCT TTCCATCACG GAGAATGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005217297.1
    CDS298..4548
    Translation

    Target ORF information:

    RefSeq Version XM_005217240.3
    Organism Bos taurus(cattle)
    Definition Bos taurus RAS protein activator like 2 (RASAL2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005217240.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGAGTCCT GGAAGATGAG CATCTTTAAG AGACAGACAT CACCGTGTCC TCCAAGCTAT 
    CGAAGATCTT TGTCAGAACC TGCTTATACA CACTCAGAGA TAGGAACAAG CTTAAAGAGT
    TATCCATGGC GTGGTCCAGT GAAAAGCAAG AGGAAGAAAA ATGAACAGGC TGTTTCCAGT
    CTAGGAAAGC CACTGGGTGC TTATCGGTGG CAAACACACC ACAGCTTAAG GTTGAAAGGG
    GACAAAAAGG AGCAATTGGA AAATAATAAA GGAGACTTGG ATGAACCACC ACAACAAGCA
    GTCCTGCCAA AGATGGTTGG GGATAATATT TCTTCCTCTG GCAATGAAGT CCTTGGGACA
    TCAATGGAGA AGCAGAACCT GGTAAACACT CAGTCACTTC GGGTTCCATT CAGCAGATCT
    CTTTCAGAGC CAGAACCCGG CTCCAGTGTC AGATTTGTTA GACGGTGGCC AACTTCAGTG
    GGGTCAGTGG AGTCTGATCA GCAGGGATAT TTCATCCGTG GCATTTTCTC TACTCTTTCC
    AATGGCTTCT CCAGGATGCT GAGTCTAAAA GGAGGGTCAA CCAGTGAGCC AGTAAGAGAA
    GATGTGAAAG GGCCACCTAC CCACCGTCTG TCTTGTGGCC AGTCGCCCTA CACCGACACA
    ACAACATGGG AGCGGAAGTA CTGCATCCTC ACAGACAGCC AGCTGGTGTT GCTCAACAAG
    GAGAAGGAGA TACCTGTGGA CGGAGGACAA GAACAGCAGG CGGATTCCAC CAAAGGGCGA
    TGCCTACGGA GAACTGTCAG TGTTCCTTCC GAGGGTCAGT TTCCTGAATA CCCACCAGAG
    GGCGCCACTA AACTGGAGGT GCCAGCAGAA AGGTCCCCAC GCAGACGGAG TATCTCAGGG
    ACCACTACAT CTGAGAAACC CAACTCTATG GACACTGCGA ATACCTCACC CTTCAAAGTA
    CCAGGGTTCT TCAGCAAGCG CCTAAAAGGC TCCATCAAGA GGACCAAAAG CCAGTCAAAG
    CTTGACCGAA ACACGAGCTT TCGGCTTCCC TCTCTTCGCA ATACCGATGA CAGGTCTCGT
    GGGCTGCCTA AACTAAAAGA GTCCCGTTCT CATGAATCTT TGCTGAGCCC ATGCAGTGCC
    GTGGAATGTC TGGATCTCGG TAGAGGGGAG CCTGTGTCGG TAAAGCCACT CCATAGCAGC
    ATCCTTGGAC AAGACTTCTG CTTTGAGGTT ACCTACTTAA GTGGAAGTAA ATGCTTCAGT
    TGTAACTCTG CTTCAGAGAG AGATAAGTGG ATGGAAAACC TGCGCAGGAC AGTTCAACCA
    AATAAGGACA ATTGTAGACG AGCTGAAAAT GTTCTCCGTT TATGGATTAT TGAAGCCAAG
    GACCTTGCCC CTAAAAAGAA ATATTTCTGT GAACTGTGCC TTGATGATAC CCTCTTTGCT
    CGTACAACTA GCAAGACCAA AGCAGACAAT ATTTTCTGGG GTGAACATTT TGAGTTCTAC
    AGCCTTCCAC CTCTTCATAG CATCACAGTT CACATTTATA AAGATGTGGA AAAAAAGAAG
    AAAAAAGACA AGAATAATTA TGTAGGGCTG GTCAACATCC CCACTGCCAG TGTGACTGGT
    CGCCAGTTTG TAGAAAAGTG GTACCCAGTG AGCACACCTA CACCCAACAA AGGAAAGACA
    GGAGGACCTT CTATTCGGAT TAAGTCGCGT TTCCAAACCA TCACCATTCT GCCTATGGAG
    CAATACAAAG AGTTTGCAGA ATTTGTCACC AGCAACTATA CCATGTTATG TTCTGTTCTT
    GAACCAGTAA TCAGTGTGAG AAATAAAGAA GAGCTAGCCT GTGCCTTAGT GCACATTCTT
    CAAAGCACTG GAAGGGCCAA GGATTTTCTG ACGGACTTGG TGATGTCTGA GGTGGATCGT
    TGTGGAGAAC ATGATGTCTT GATCTTCAGA GAGAACACCA TTGCCACCAA ATCCATTGAG
    GAATACCTCA AGTTGGTGGG ACAACAGTAT CTTCACGATG CACTGGGGGA GTTCATCAAA
    GCGTTGTATG AGTCAGATGA AAACTGTGAA GTGGATCCCA GCAAATGTTC ATCCAGTGAA
    CTTCTGGACC ATCAGAGCAA CCTGAAAATG TGCTGTGAGC TGGCTTTCTG CAAGATCATC
    AACTCTTACT GTGTTTTTCC TCGTGAGCTG AAAGAAGTGT TTGCATCCTG GAAGCAGCAG
    TGCCTGAACC GCGGCAAGCA GGACATCAGC GAGAGGCTCA TCAGCGCCTC GCTGTTTCTC
    CGTTTTCTGT GCCCGGCCAT CATGTCTCCC AGTCTCTTCA ACCTCATGCA GGAGTATCCT
    GATGACCGCA CATCTCGGAC TCTGACTCTC ATTGCCAAGG TCATCCAGAA CCTGGCCAAC
    TTTGCCAAAT TTGGTAACAA GGAGGAATAC ATGGCATTCA TGAATGATTT TTTAGAACAT
    GAATGGGGTG GAATGAAGCG CTTTCTTTTG GAGATCTCTA ATCCAGACAC CATCTCAAAC
    ACCCCAGGCT TTGATGGTTA CATTGATCTG GGCCGAGAGC TTTCAGTTTT GCATTCCTTA
    CTATGGGAAG TAGTTTCCCA ACTTGATAAG GCAACCGTGG CAAAATTGGG ACCTCTCCCT
    CGTGTTCTTG CTGATATTAC CAAATCTCTG ACTAATCCTA CACCAATACA ACAGCAACTG
    AGACGCTTCA CTGAGCATAA CTCCAGTCCA AATGTCAGTG GAAGCCTCTC CTCTGGGCTG
    CAGAAAATAT TTGAAGACCC TACTGACAGT GATTTGCACA AACTAAAATC CCCAAGCCAG
    GACAACACAG ACAGTTATTT CAGAGGGAAG ACATTATTGC TGGTCCAGCA AGCCTCCTCC
    CAGAGCATGA CTTACTCGGA AAAGGATGAA AAGGAAAGTA GCCTCCCCAA TGGCCGCAGC
    ATCTCCCTCA TGGACCTTCA GGAGACGCAC GCAGCCCAAG CAGAGCATCC ATCTGTGATA
    CTCGACGTGC CCATGCGCTT GACCGGAAGC CAGCTCTCCA TAACTCAGGT GGCCAGCATC
    AAGCAACTGC GGGAGACCCA GAGCACCCCC CAGAGTGCAC CCCAAGTGCG AAGGCCCCTG
    CACCCAGCCT TGAACCAGCC AGGCAGCCTC CAGCCCCTGT CCTTCCAGAA CCCTGTCTAT
    CACCTCAGTA ACCCCATTCC TGCAATGCCA AAGGCCTCTG TGGACTCCAG TTTGGAGAAC
    CTAAGCACTG CCAGTTCCAG GAGCCAAAGT AACAGTGAAG ACTTCAAACT CAGCGGACCC
    AGCAACAGCA GCATGGAGGA CTTCACCAAA CGGAGCACGC AGAGTGAGGA CTTCTCCAGG
    CGCCACACCA TGCCCGACAG ACACATACCT CTCGCCCTGC CGCGGCAGAA CAGCACGGGG
    CAGACCCAGC TCCGGAAGAT GGACCCGGCG GGGCTGGGCG CCCGAGCCAA AGCCCCGCCG
    TCCCTGCCGC ACAGCGCCTC CCTGCGGAGC ACCGGGAGCA TGTCGGTGGC ATCAGCGGCT
    CTGGTGGCCG AGCCCCTGCA GAACGGGAGC CGGTCCCGAC AGCAGTCCTC TTCCTCCAGA
    GAGAGCCCGG TCCCCAAAGT GAGAGCCATC CAGAGGCAGC AGACCCAGCA GGTTCAGTCA
    CCTGTGGACT CCGCCACAAT GTCCCCAGTC GAGAGGACAG CAGCCTGGGT CCTGAACAAT
    GGGCAGTATG AAGAGGACGT GGAAGAGACT GAGCAAAATC AGGACGAAGC CAAGCATGCT
    GAGAAGTACG AACAGGAAAT CACCAAACTG AAGGAGCGCC TGCGCGTGTC CAGCCGGCGA
    CTCGAGGAGT ATGAGCGCCG GTTGCTAGTG CAGGAGCAGC AGATGCAGAA GCTGCTGCTG
    GAGTACAAGG CCCGGCTGGA GGACAGCGAG GAGCGGCTGC GCAGGCAGCA GGAGGAGAAG
    GACAGCCAGA TGAAGAGCAT CATCAGCAGG CTAATGGCTG TGGAAGAGGA ACTGAAGAAG
    GATCATGCTG AGATGCAGGC AGTTATTGAT GCAAAGCAAA AAATAATCGA TGCACAGGTC
    ATAAATGGAG ATGAGATTAT GCAAACAGGA AAAACGGATC GTGTCGCTGG ATTCGGCCAA
    CACCAGACTG ATGAGTGCGC TGACCCAGGT GAAGGAACGG TACAGCATGC AGGTCCGAAA
    TGGCATCTCC CCCACCAACC CCACCAAGCT TTCCATCACG GAGAATGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27406
    Clone ID Related Accession (Same CDS sequence) XM_005217240.3 , XM_005194888.3
    Accession Version XM_005194888.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4251bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ras GTPase-activating protein nGAP isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646324.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005194888.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGAGTCCT GGAAGATGAG CATCTTTAAG AGACAGACAT CACCGTGTCC TCCAAGCTAT 
    CGAAGATCTT TGTCAGAACC TGCTTATACA CACTCAGAGA TAGGAACAAG CTTAAAGAGT
    TATCCATGGC GTGGTCCAGT GAAAAGCAAG AGGAAGAAAA ATGAACAGGC TGTTTCCAGT
    CTAGGAAAGC CACTGGGTGC TTATCGGTGG CAAACACACC ACAGCTTAAG GTTGAAAGGG
    GACAAAAAGG AGCAATTGGA AAATAATAAA GGAGACTTGG ATGAACCACC ACAACAAGCA
    GTCCTGCCAA AGATGGTTGG GGATAATATT TCTTCCTCTG GCAATGAAGT CCTTGGGACA
    TCAATGGAGA AGCAGAACCT GGTAAACACT CAGTCACTTC GGGTTCCATT CAGCAGATCT
    CTTTCAGAGC CAGAACCCGG CTCCAGTGTC AGATTTGTTA GACGGTGGCC AACTTCAGTG
    GGGTCAGTGG AGTCTGATCA GCAGGGATAT TTCATCCGTG GCATTTTCTC TACTCTTTCC
    AATGGCTTCT CCAGGATGCT GAGTCTAAAA GGAGGGTCAA CCAGTGAGCC AGTAAGAGAA
    GATGTGAAAG GGCCACCTAC CCACCGTCTG TCTTGTGGCC AGTCGCCCTA CACCGACACA
    ACAACATGGG AGCGGAAGTA CTGCATCCTC ACAGACAGCC AGCTGGTGTT GCTCAACAAG
    GAGAAGGAGA TACCTGTGGA CGGAGGACAA GAACAGCAGG CGGATTCCAC CAAAGGGCGA
    TGCCTACGGA GAACTGTCAG TGTTCCTTCC GAGGGTCAGT TTCCTGAATA CCCACCAGAG
    GGCGCCACTA AACTGGAGGT GCCAGCAGAA AGGTCCCCAC GCAGACGGAG TATCTCAGGG
    ACCACTACAT CTGAGAAACC CAACTCTATG GACACTGCGA ATACCTCACC CTTCAAAGTA
    CCAGGGTTCT TCAGCAAGCG CCTAAAAGGC TCCATCAAGA GGACCAAAAG CCAGTCAAAG
    CTTGACCGAA ACACGAGCTT TCGGCTTCCC TCTCTTCGCA ATACCGATGA CAGGTCTCGT
    GGGCTGCCTA AACTAAAAGA GTCCCGTTCT CATGAATCTT TGCTGAGCCC ATGCAGTGCC
    GTGGAATGTC TGGATCTCGG TAGAGGGGAG CCTGTGTCGG TAAAGCCACT CCATAGCAGC
    ATCCTTGGAC AAGACTTCTG CTTTGAGGTT ACCTACTTAA GTGGAAGTAA ATGCTTCAGT
    TGTAACTCTG CTTCAGAGAG AGATAAGTGG ATGGAAAACC TGCGCAGGAC AGTTCAACCA
    AATAAGGACA ATTGTAGACG AGCTGAAAAT GTTCTCCGTT TATGGATTAT TGAAGCCAAG
    GACCTTGCCC CTAAAAAGAA ATATTTCTGT GAACTGTGCC TTGATGATAC CCTCTTTGCT
    CGTACAACTA GCAAGACCAA AGCAGACAAT ATTTTCTGGG GTGAACATTT TGAGTTCTAC
    AGCCTTCCAC CTCTTCATAG CATCACAGTT CACATTTATA AAGATGTGGA AAAAAAGAAG
    AAAAAAGACA AGAATAATTA TGTAGGGCTG GTCAACATCC CCACTGCCAG TGTGACTGGT
    CGCCAGTTTG TAGAAAAGTG GTACCCAGTG AGCACACCTA CACCCAACAA AGGAAAGACA
    GGAGGACCTT CTATTCGGAT TAAGTCGCGT TTCCAAACCA TCACCATTCT GCCTATGGAG
    CAATACAAAG AGTTTGCAGA ATTTGTCACC AGCAACTATA CCATGTTATG TTCTGTTCTT
    GAACCAGTAA TCAGTGTGAG AAATAAAGAA GAGCTAGCCT GTGCCTTAGT GCACATTCTT
    CAAAGCACTG GAAGGGCCAA GGATTTTCTG ACGGACTTGG TGATGTCTGA GGTGGATCGT
    TGTGGAGAAC ATGATGTCTT GATCTTCAGA GAGAACACCA TTGCCACCAA ATCCATTGAG
    GAATACCTCA AGTTGGTGGG ACAACAGTAT CTTCACGATG CACTGGGGGA GTTCATCAAA
    GCGTTGTATG AGTCAGATGA AAACTGTGAA GTGGATCCCA GCAAATGTTC ATCCAGTGAA
    CTTCTGGACC ATCAGAGCAA CCTGAAAATG TGCTGTGAGC TGGCTTTCTG CAAGATCATC
    AACTCTTACT GTGTTTTTCC TCGTGAGCTG AAAGAAGTGT TTGCATCCTG GAAGCAGCAG
    TGCCTGAACC GCGGCAAGCA GGACATCAGC GAGAGGCTCA TCAGCGCCTC GCTGTTTCTC
    CGTTTTCTGT GCCCGGCCAT CATGTCTCCC AGTCTCTTCA ACCTCATGCA GGAGTATCCT
    GATGACCGCA CATCTCGGAC TCTGACTCTC ATTGCCAAGG TCATCCAGAA CCTGGCCAAC
    TTTGCCAAAT TTGGTAACAA GGAGGAATAC ATGGCATTCA TGAATGATTT TTTAGAACAT
    GAATGGGGTG GAATGAAGCG CTTTCTTTTG GAGATCTCTA ATCCAGACAC CATCTCAAAC
    ACCCCAGGCT TTGATGGTTA CATTGATCTG GGCCGAGAGC TTTCAGTTTT GCATTCCTTA
    CTATGGGAAG TAGTTTCCCA ACTTGATAAG GCAACCGTGG CAAAATTGGG ACCTCTCCCT
    CGTGTTCTTG CTGATATTAC CAAATCTCTG ACTAATCCTA CACCAATACA ACAGCAACTG
    AGACGCTTCA CTGAGCATAA CTCCAGTCCA AATGTCAGTG GAAGCCTCTC CTCTGGGCTG
    CAGAAAATAT TTGAAGACCC TACTGACAGT GATTTGCACA AACTAAAATC CCCAAGCCAG
    GACAACACAG ACAGTTATTT CAGAGGGAAG ACATTATTGC TGGTCCAGCA AGCCTCCTCC
    CAGAGCATGA CTTACTCGGA AAAGGATGAA AAGGAAAGTA GCCTCCCCAA TGGCCGCAGC
    ATCTCCCTCA TGGACCTTCA GGAGACGCAC GCAGCCCAAG CAGAGCATCC ATCTGTGATA
    CTCGACGTGC CCATGCGCTT GACCGGAAGC CAGCTCTCCA TAACTCAGGT GGCCAGCATC
    AAGCAACTGC GGGAGACCCA GAGCACCCCC CAGAGTGCAC CCCAAGTGCG AAGGCCCCTG
    CACCCAGCCT TGAACCAGCC AGGCAGCCTC CAGCCCCTGT CCTTCCAGAA CCCTGTCTAT
    CACCTCAGTA ACCCCATTCC TGCAATGCCA AAGGCCTCTG TGGACTCCAG TTTGGAGAAC
    CTAAGCACTG CCAGTTCCAG GAGCCAAAGT AACAGTGAAG ACTTCAAACT CAGCGGACCC
    AGCAACAGCA GCATGGAGGA CTTCACCAAA CGGAGCACGC AGAGTGAGGA CTTCTCCAGG
    CGCCACACCA TGCCCGACAG ACACATACCT CTCGCCCTGC CGCGGCAGAA CAGCACGGGG
    CAGACCCAGC TCCGGAAGAT GGACCCGGCG GGGCTGGGCG CCCGAGCCAA AGCCCCGCCG
    TCCCTGCCGC ACAGCGCCTC CCTGCGGAGC ACCGGGAGCA TGTCGGTGGC ATCAGCGGCT
    CTGGTGGCCG AGCCCCTGCA GAACGGGAGC CGGTCCCGAC AGCAGTCCTC TTCCTCCAGA
    GAGAGCCCGG TCCCCAAAGT GAGAGCCATC CAGAGGCAGC AGACCCAGCA GGTTCAGTCA
    CCTGTGGACT CCGCCACAAT GTCCCCAGTC GAGAGGACAG CAGCCTGGGT CCTGAACAAT
    GGGCAGTATG AAGAGGACGT GGAAGAGACT GAGCAAAATC AGGACGAAGC CAAGCATGCT
    GAGAAGTACG AACAGGAAAT CACCAAACTG AAGGAGCGCC TGCGCGTGTC CAGCCGGCGA
    CTCGAGGAGT ATGAGCGCCG GTTGCTAGTG CAGGAGCAGC AGATGCAGAA GCTGCTGCTG
    GAGTACAAGG CCCGGCTGGA GGACAGCGAG GAGCGGCTGC GCAGGCAGCA GGAGGAGAAG
    GACAGCCAGA TGAAGAGCAT CATCAGCAGG CTAATGGCTG TGGAAGAGGA ACTGAAGAAG
    GATCATGCTG AGATGCAGGC AGTTATTGAT GCAAAGCAAA AAATAATCGA TGCACAGGTC
    ATAAATGGAG ATGAGATTAT GCAAACAGGA AAAACGGATC GTGTCGCTGG ATTCGGCCAA
    CACCAGACTG ATGAGTGCGC TGACCCAGGT GAAGGAACGG TACAGCATGC AGGTCCGAAA
    TGGCATCTCC CCCACCAACC CCACCAAGCT TTCCATCACG GAGAATGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005194945.1
    CDS299..4549
    Translation

    Target ORF information:

    RefSeq Version XM_005194888.3
    Organism Bos taurus(cattle)
    Definition Bos taurus RAS protein activator like 2 (RASAL2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005194888.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGAGTCCT GGAAGATGAG CATCTTTAAG AGACAGACAT CACCGTGTCC TCCAAGCTAT 
    CGAAGATCTT TGTCAGAACC TGCTTATACA CACTCAGAGA TAGGAACAAG CTTAAAGAGT
    TATCCATGGC GTGGTCCAGT GAAAAGCAAG AGGAAGAAAA ATGAACAGGC TGTTTCCAGT
    CTAGGAAAGC CACTGGGTGC TTATCGGTGG CAAACACACC ACAGCTTAAG GTTGAAAGGG
    GACAAAAAGG AGCAATTGGA AAATAATAAA GGAGACTTGG ATGAACCACC ACAACAAGCA
    GTCCTGCCAA AGATGGTTGG GGATAATATT TCTTCCTCTG GCAATGAAGT CCTTGGGACA
    TCAATGGAGA AGCAGAACCT GGTAAACACT CAGTCACTTC GGGTTCCATT CAGCAGATCT
    CTTTCAGAGC CAGAACCCGG CTCCAGTGTC AGATTTGTTA GACGGTGGCC AACTTCAGTG
    GGGTCAGTGG AGTCTGATCA GCAGGGATAT TTCATCCGTG GCATTTTCTC TACTCTTTCC
    AATGGCTTCT CCAGGATGCT GAGTCTAAAA GGAGGGTCAA CCAGTGAGCC AGTAAGAGAA
    GATGTGAAAG GGCCACCTAC CCACCGTCTG TCTTGTGGCC AGTCGCCCTA CACCGACACA
    ACAACATGGG AGCGGAAGTA CTGCATCCTC ACAGACAGCC AGCTGGTGTT GCTCAACAAG
    GAGAAGGAGA TACCTGTGGA CGGAGGACAA GAACAGCAGG CGGATTCCAC CAAAGGGCGA
    TGCCTACGGA GAACTGTCAG TGTTCCTTCC GAGGGTCAGT TTCCTGAATA CCCACCAGAG
    GGCGCCACTA AACTGGAGGT GCCAGCAGAA AGGTCCCCAC GCAGACGGAG TATCTCAGGG
    ACCACTACAT CTGAGAAACC CAACTCTATG GACACTGCGA ATACCTCACC CTTCAAAGTA
    CCAGGGTTCT TCAGCAAGCG CCTAAAAGGC TCCATCAAGA GGACCAAAAG CCAGTCAAAG
    CTTGACCGAA ACACGAGCTT TCGGCTTCCC TCTCTTCGCA ATACCGATGA CAGGTCTCGT
    GGGCTGCCTA AACTAAAAGA GTCCCGTTCT CATGAATCTT TGCTGAGCCC ATGCAGTGCC
    GTGGAATGTC TGGATCTCGG TAGAGGGGAG CCTGTGTCGG TAAAGCCACT CCATAGCAGC
    ATCCTTGGAC AAGACTTCTG CTTTGAGGTT ACCTACTTAA GTGGAAGTAA ATGCTTCAGT
    TGTAACTCTG CTTCAGAGAG AGATAAGTGG ATGGAAAACC TGCGCAGGAC AGTTCAACCA
    AATAAGGACA ATTGTAGACG AGCTGAAAAT GTTCTCCGTT TATGGATTAT TGAAGCCAAG
    GACCTTGCCC CTAAAAAGAA ATATTTCTGT GAACTGTGCC TTGATGATAC CCTCTTTGCT
    CGTACAACTA GCAAGACCAA AGCAGACAAT ATTTTCTGGG GTGAACATTT TGAGTTCTAC
    AGCCTTCCAC CTCTTCATAG CATCACAGTT CACATTTATA AAGATGTGGA AAAAAAGAAG
    AAAAAAGACA AGAATAATTA TGTAGGGCTG GTCAACATCC CCACTGCCAG TGTGACTGGT
    CGCCAGTTTG TAGAAAAGTG GTACCCAGTG AGCACACCTA CACCCAACAA AGGAAAGACA
    GGAGGACCTT CTATTCGGAT TAAGTCGCGT TTCCAAACCA TCACCATTCT GCCTATGGAG
    CAATACAAAG AGTTTGCAGA ATTTGTCACC AGCAACTATA CCATGTTATG TTCTGTTCTT
    GAACCAGTAA TCAGTGTGAG AAATAAAGAA GAGCTAGCCT GTGCCTTAGT GCACATTCTT
    CAAAGCACTG GAAGGGCCAA GGATTTTCTG ACGGACTTGG TGATGTCTGA GGTGGATCGT
    TGTGGAGAAC ATGATGTCTT GATCTTCAGA GAGAACACCA TTGCCACCAA ATCCATTGAG
    GAATACCTCA AGTTGGTGGG ACAACAGTAT CTTCACGATG CACTGGGGGA GTTCATCAAA
    GCGTTGTATG AGTCAGATGA AAACTGTGAA GTGGATCCCA GCAAATGTTC ATCCAGTGAA
    CTTCTGGACC ATCAGAGCAA CCTGAAAATG TGCTGTGAGC TGGCTTTCTG CAAGATCATC
    AACTCTTACT GTGTTTTTCC TCGTGAGCTG AAAGAAGTGT TTGCATCCTG GAAGCAGCAG
    TGCCTGAACC GCGGCAAGCA GGACATCAGC GAGAGGCTCA TCAGCGCCTC GCTGTTTCTC
    CGTTTTCTGT GCCCGGCCAT CATGTCTCCC AGTCTCTTCA ACCTCATGCA GGAGTATCCT
    GATGACCGCA CATCTCGGAC TCTGACTCTC ATTGCCAAGG TCATCCAGAA CCTGGCCAAC
    TTTGCCAAAT TTGGTAACAA GGAGGAATAC ATGGCATTCA TGAATGATTT TTTAGAACAT
    GAATGGGGTG GAATGAAGCG CTTTCTTTTG GAGATCTCTA ATCCAGACAC CATCTCAAAC
    ACCCCAGGCT TTGATGGTTA CATTGATCTG GGCCGAGAGC TTTCAGTTTT GCATTCCTTA
    CTATGGGAAG TAGTTTCCCA ACTTGATAAG GCAACCGTGG CAAAATTGGG ACCTCTCCCT
    CGTGTTCTTG CTGATATTAC CAAATCTCTG ACTAATCCTA CACCAATACA ACAGCAACTG
    AGACGCTTCA CTGAGCATAA CTCCAGTCCA AATGTCAGTG GAAGCCTCTC CTCTGGGCTG
    CAGAAAATAT TTGAAGACCC TACTGACAGT GATTTGCACA AACTAAAATC CCCAAGCCAG
    GACAACACAG ACAGTTATTT CAGAGGGAAG ACATTATTGC TGGTCCAGCA AGCCTCCTCC
    CAGAGCATGA CTTACTCGGA AAAGGATGAA AAGGAAAGTA GCCTCCCCAA TGGCCGCAGC
    ATCTCCCTCA TGGACCTTCA GGAGACGCAC GCAGCCCAAG CAGAGCATCC ATCTGTGATA
    CTCGACGTGC CCATGCGCTT GACCGGAAGC CAGCTCTCCA TAACTCAGGT GGCCAGCATC
    AAGCAACTGC GGGAGACCCA GAGCACCCCC CAGAGTGCAC CCCAAGTGCG AAGGCCCCTG
    CACCCAGCCT TGAACCAGCC AGGCAGCCTC CAGCCCCTGT CCTTCCAGAA CCCTGTCTAT
    CACCTCAGTA ACCCCATTCC TGCAATGCCA AAGGCCTCTG TGGACTCCAG TTTGGAGAAC
    CTAAGCACTG CCAGTTCCAG GAGCCAAAGT AACAGTGAAG ACTTCAAACT CAGCGGACCC
    AGCAACAGCA GCATGGAGGA CTTCACCAAA CGGAGCACGC AGAGTGAGGA CTTCTCCAGG
    CGCCACACCA TGCCCGACAG ACACATACCT CTCGCCCTGC CGCGGCAGAA CAGCACGGGG
    CAGACCCAGC TCCGGAAGAT GGACCCGGCG GGGCTGGGCG CCCGAGCCAA AGCCCCGCCG
    TCCCTGCCGC ACAGCGCCTC CCTGCGGAGC ACCGGGAGCA TGTCGGTGGC ATCAGCGGCT
    CTGGTGGCCG AGCCCCTGCA GAACGGGAGC CGGTCCCGAC AGCAGTCCTC TTCCTCCAGA
    GAGAGCCCGG TCCCCAAAGT GAGAGCCATC CAGAGGCAGC AGACCCAGCA GGTTCAGTCA
    CCTGTGGACT CCGCCACAAT GTCCCCAGTC GAGAGGACAG CAGCCTGGGT CCTGAACAAT
    GGGCAGTATG AAGAGGACGT GGAAGAGACT GAGCAAAATC AGGACGAAGC CAAGCATGCT
    GAGAAGTACG AACAGGAAAT CACCAAACTG AAGGAGCGCC TGCGCGTGTC CAGCCGGCGA
    CTCGAGGAGT ATGAGCGCCG GTTGCTAGTG CAGGAGCAGC AGATGCAGAA GCTGCTGCTG
    GAGTACAAGG CCCGGCTGGA GGACAGCGAG GAGCGGCTGC GCAGGCAGCA GGAGGAGAAG
    GACAGCCAGA TGAAGAGCAT CATCAGCAGG CTAATGGCTG TGGAAGAGGA ACTGAAGAAG
    GATCATGCTG AGATGCAGGC AGTTATTGAT GCAAAGCAAA AAATAATCGA TGCACAGGTC
    ATAAATGGAG ATGAGATTAT GCAAACAGGA AAAACGGATC GTGTCGCTGG ATTCGGCCAA
    CACCAGACTG ATGAGTGCGC TGACCCAGGT GAAGGAACGG TACAGCATGC AGGTCCGAAA
    TGGCATCTCC CCCACCAACC CCACCAAGCT TTCCATCACG GAGAATGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.