RASAL2 gene cDNA ORF clone, Canis lupus familiaris(dog)

mRNA	Protein	Name
XM_014115155.2	XP_013970630.1	ras GTPase-activating protein nGAP isoform X5
XM_014115159.2	XP_013970634.1	ras GTPase-activating protein nGAP isoform X12
XM_005622480.2	XP_005622537.1	ras GTPase-activating protein nGAP isoform X5
XM_014115158.1	XP_013970633.1	ras GTPase-activating protein nGAP isoform X9
XM_014115160.1	XP_013970635.1	ras GTPase-activating protein nGAP isoform X11
XM_014115154.1	XP_013970629.1	ras GTPase-activating protein nGAP isoform X1
XM_005622478.2	XP_005622535.1	ras GTPase-activating protein nGAP isoform X2
XM_005622479.2	XP_005622536.1	ras GTPase-activating protein nGAP isoform X3
XM_014115161.1	XP_013970636.1	ras GTPase-activating protein nGAP isoform X11
XM_014115156.2	XP_013970631.2	ras GTPase-activating protein nGAP isoform X9
XM_014115159.1	XP_013970634.1	ras GTPase-activating protein nGAP isoform X10
XM_022421007.1	XP_022276715.1	ras GTPase-activating protein nGAP isoform X4
XM_022421008.1	XP_022276716.1	ras GTPase-activating protein nGAP isoform X8
XM_022421009.1	XP_022276717.1	ras GTPase-activating protein nGAP isoform X10
XM_014115155.1	XP_013970630.1	ras GTPase-activating protein nGAP isoform X4
XM_003434980.3	XP_003435028.2	ras GTPase-activating protein nGAP isoform X6
XM_014115156.1	XP_013970631.1	ras GTPase-activating protein nGAP isoform X7
XM_014115157.1	XP_013970632.1	ras GTPase-activating protein nGAP isoform X8
XM_014115154.2	XP_013970629.1	ras GTPase-activating protein nGAP isoform X1
XM_005622478.3	XP_005622535.1	ras GTPase-activating protein nGAP isoform X2
XM_005622479.3	XP_005622536.1	ras GTPase-activating protein nGAP isoform X3
XM_005622480.3	XP_005622537.1	ras GTPase-activating protein nGAP isoform X6
XM_014115158.2	XP_013970633.1	ras GTPase-activating protein nGAP isoform X11
XM_003434980.4	XP_003435028.2	ras GTPase-activating protein nGAP isoform X7

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe289530	XM_022421007.1 Latest version!	Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCe289531	XM_022421008.1 Latest version!	Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCe289532	XM_014115156.2 Latest version!	Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCe289533	XM_022421009.1 Latest version!	Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCe37436	XM_014115154.2 Latest version!	Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCe37437	XM_014115155.2 Latest version!	Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCe37438	XM_014115156.1 Latest version!	Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCe37439	XM_014115157.1 Latest version!	Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCe37440	XM_014115158.2 Latest version!	Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCe37441	XM_014115159.2 Latest version!	Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCe37442	XM_014115161.1 Latest version!	Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCe37442	XM_014115160.1 Latest version!	Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCe09201	XM_005622480.3 Latest version!	Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OCe09202	XM_003434980.4 Latest version!	Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OCe09199	XM_005622478.3 Latest version!	Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OCe09200	XM_005622479.3 Latest version!	Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
View Old Accession Versions >>
OCe37441	XM_014115159.1	Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCe37437	XM_014115155.1	Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCe37436	XM_014115154.1	Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCe09199	XM_005622478.2	Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OCe09200	XM_005622479.2	Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OCe09201	XM_005622480.2	Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OCe37440	XM_014115158.1	Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCe09202	XM_003434980.3	Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
Hide Old Accession Versions >>

RASAL2 ( XM_022421007.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022276715.1 Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe289530
Clone ID Related Accession (Same CDS sequence)	XM_022421007.1
Accession Version	XM_022421007.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4110bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	ras GTPase-activating protein nGAP isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTCGT GGAAGATGAG CATCTTTAAG AGACAGACAT CACCTTGTCC TCCAAGCTAT 
CGAAGATCTT TGTCAGAACC TGCTTATGCA AAATCAGAGA TAGGAATAAG CTTAAAGAGT 
TATCCATGGC GTGGTCCAGT GAAAATCAAG AGGAAGAAAA ATGAACATGC TGTTTCCAGT 
CTAGGAAAGC CACTGGGTGC TTATCGGTGG CAAACACATC CCAGCTGTAG GTGGAAAGGG 
GGCAAAAGGG AGAGGTTACA GACTAATAAA AGAGACTTGG ATGAACGGCA ACAAAAGGTC 
CTGCCAGAGA TGGTTGGTAG TAATGCTTCT TCCTCTGCTA TTGAGGTCCT TGGAGCACCA 
ACAGAGAAGC TGAACCTGGT CAACACCCGG TCACTTCGGG TTCCATTCAC CAGATCTATT 
TCAGAGCCAG AACCTCGCTG CAGTATCAGA TTTGTTAGAT CATGGCCGAC TACAGGAAGG 
TCAGAGGAGT CTGAACAGCA GGGATATTTC ATCCGTGGCA TTTTCTCTAC TCTTTCCAAT 
GGCTTCTCCA GGATGCTGAG TCTGAAAGGA GAATCAACCA ATGAGCCAGT AAGAGAAGAT 
GTGAAGGGGC CACCTACCCA TCGTCTGTCT TGTGGACAGT CGCCCTACAC AGAGACGACA 
ACATGGGAGC GGAAGTATTG CATCCTCACC GACAGCCAGT TGGTGTTGCT CAACAAGGAG 
AAGGAGATAC CTGTGGAGGG AGGACAGGAG CAGCAGACAG ATTCCACCAA AGGGCGATGC 
CTGAGGAGAA CCGTCAGTGT CCCTTCCGAG GGCCAGTTTC CTGAGTACCC ACCAGAGGGC 
GCCACCAAAC TGGAGGTGCC GGCAGAAAGG TCCCCACGCA GACGGAGTAT CTCAGGGACC 
AGTACATCAG AGAAACCCAA CTCCATGGAC ACTGTAAATA CCTCACCCTT CAAAGTACCA 
GGGTTCTTCA GCAAGCGCCT GAAAGGCTCC ATCAAGAGGA CCAAAAGCCA ATCAAAGCTT 
GACAGAAACA CGAGCTTTCG GCTACCATCC CTTCGTAATG CAGATGACAG GACTCGCGGT 
CTGCCTAAAC TAAAAGAGTC ACGTTCCCAT GAGTCATTGC TGAGCCCTTG TAGTGCAGTG 
GAATGTCTGG ATCTTGGCAG AGGAGAGCCT GTGTCAGTGA AGCCACTCCA TAGCAGCATC 
CTTGGACAAG ACTTCTGCTT TGAGGTTACG TACTTAAGTG GAAGTAAATG CTTCAGCTGT 
AACTCTGCTT CAGAGAGAGA TAAGTGGATG GAGAACCTTC GTAGGACAGT TCAACCAAAT 
AAGGACAATT GCAGACGAGC TGAAAACGTT CTTCGTTTAT GGATCATTGA AGCCAAGGAC 
CTTGCCCCCA AAAAGAAATA TTTCTGTGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT 
ACAACCAGCA AGACCAAAGC AGATAATATT TTCTGGGGTG AACATTTTGA ATTTTACAGC 
CTTCCACCTC TTCATAGCAT CACAGTTCAC ATTTATAAAG ATGTGGAAAA AAAGAAAAAA 
AAAGACAAGA ATAATTATGT AGGGCTAGTC AACATTCCCA CTGCCAGTGT GACTGGGCGC 
CAATTTGTAG AAAAGTGGTA TCCAGTGAGT ACCCCTACAC CCAACAAAGG AAAGACCGGA 
GGACCTTCTA TTCGGATTAA ATCACGTTTC CAAACTATCA CCATTCTGCC TATGGAGCAA 
TACAAAGAAT TTGCAGAATT TGTCACCAGC AACTATACCA TGCTGTGTTC TGTCCTTGAA 
CCAGTAATTA GTGTGAGAAA TAAAGAGGAG TTGGCCTGTG CCCTAGTGCA CATTCTTCAA 
AGTACTGGCA GAGCCAAGGA TTTTCTGACC GACTTGGTGA TGTCTGAGGT GGATCGCTGT 
GGAGAGCATG ACGTCTTGAT CTTCAGAGAG AACACCATTG CTACCAAATC CATTGAGGAA 
TACCTCAAGT TGGTGGGACA ACAGTATCTT CATGACGCAT TGGGGGAGTT CATCAAAGCT 
TTGTACGAGT CAGATGAGAA CTGTGAAGTG GATCCCAGCA AGTGTTCCTC TAGTGAACTG 
ATAGACCATC AGAGCAACCT GAAGATGTGC TGTGAGCTGG CTTTCTGCAA GATCATCAAC 
TCTTACTGTG TTTTCCCTCG TGAGTTGAAA GAAGTGTTTG CATCGTGGAA GCAGCAGTGC 
CTGAACCGTG GCAAACAGGA CATCAGCGAG CGGCTCATCA GTGCCTCATT GTTTCTCCGT 
TTCCTGTGCC CTGCCATCAT GTCTCCCAGT CTCTTCAACC TTATGCAAGA GTACCCTGAT 
GACCGTACAT CTCGCACCCT GACTCTGATT GCCAAGGTCA TTCAGAACCT GGCTAACTTT 
GCCAAGTTTG GTAACAAAGA AGAATATATG GCATTCATGA ATGATTTTTT AGAACATGAA 
TGGGGTGGAA TGAAGCGCTT TCTTTTGGAA ATCTCTAATC CGGACACCAT CTCAAACACC 
CCAGGCTTTG ATGGTTACAT TGATCTGGGC CGAGAGCTTT CAGTCTTGCA TTCCTTACTA 
TGGGAAGTAG TTTCCCAGCT TGATAAGGGT GAAAATTCCT TCCTACAGGC GACCGTGGCA 
AAATTGGGAC CGCTCCCTCG AGTTCTTGCT GATATTACCA AGTCTCTAAC TAATCCTACA 
CCAATACAAC AGCAGCTGAG ACGCTTCACT GAGCATAACT CCAGTCCAAA TGTCAGTGGA 
AGCCTCTCCT CTGGGCTGCA GAAAATATTT GAAGACCCCA CTGACAGTGA TTTGCATAAA 
CTAAAATCTC CAAGCCAGGA CAACACAGAT GGCTATTTCA GAGGGAAAAC GTTATTGCTG 
GTTCAGCAGG CCTCCTCCCA GAGCATGACC TATTCTGAAA AGGATGAAAA GGAAAGTAGC 
CTTCCTAACG GTCGGAGCAT CTCCCTCATG GACCTTCAAG ACACTCACGC TGCTCAAGTA 
GAGCATGCAT CTATCATGCT CGATGTGCCT ATGCGCTTAA CTGGAAGCCA GCTTTCCATA 
ACCCAGGTGG CCAGTATCAA ACAACTGCGG GAAACCCAGA GCACTCCCCA GAGTGCACCC 
CAAGTGCGAA GGCCCTTGCA CCCAGCCTTG AACCAGCCAG GCAGCCTCCA GCCCCTGTCA 
TTCCAGAACC CTGTCTATCA CCTCAATAAC CCAATCCCAG CAATGCCAAA GGCCTCTGTG 
GATTCTAGTT TGGAGAATCT AAGCACTGCC AGTTCCAGAA GCCAAAGTAA CAGTGAAGAT 
TTCAAACTCA GTGGGCCCAG CAATAGCAGC ATGGAAGATT TCACTAAACG TAGCACGCAG 
AGCGAGGATT TCTCCAGGCG GCACACTGTA CCAGACAGAC ACATACCTCT TGCTCTGCCG 
CGGCAGAATA GTACCGGGCA GGCCCAGATC CGGAAAATGG ACCAGGCTGG GTTAGGTGCC 
CGAGCCAAAG CCCCGCCGTC CTTGCCACAC AGTGCTTCCT TACGTAGCAC GGGGAGCATG 
TCGGTGGCCT CTGCGGCCCT GGTGGCTGAA CCTGTGCAGA ATGGGAGCCG GTCCCGGCAG 
CAGTCCTCTT CCTCCAGAGA GAGCCCCGTT CCCAAAGTGA GAGCGATCCA GAGACAACAG 
ACACAGCAGG TGCAGTCACC TGTGGACTCT GCCACCATGT CCCCAGTAGA GAGGACAGCA 
GCCTGGGTGT TGAACAATGG ACAGTATGAA GAGGATGTGG AAGAGACTGA GCAAAATCCA 
GATGAATCCA AGCATGCTGA GAAGTATGAG CAGGAAATTA CTAAACTGAA GGAGCGCCTG 
AGAGTGTCCA GCCGGCGCCT TGAGGAATAT GAGCGCCGCT TGCTGGTGCA GGAGCAGCAG 
ATGCAGAAGC TGCTGCTGGA ATACAAGGCC AGGCTGGAGG ACAGCGAGGA GCGCCTGCGC 
AGGCAGCAGG AGGAGAAGGA CAGCCAGATG AAAAGCATCA TCAGCAGGCT GATGGCTGTG 
GAAGAGGAAC TGAAGAAGGA TCATGCTGAG ATGCAAGCAG TTATTGATGC AAAGCAAAAA 
ATAATCGATG CACAGAGGAG TTGGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022276715.1
CDS	310..4419
Translation	MESWKMSIFKRQTSPCPPSYRRSLSEPAYAKSEIGISLKSYPWRGPVKIKRKKNEHAVSSLGKPLGAYRWQTHPSCRWKGGKRERLQTNKRDLDERQQKVLPEMVGSNASSSAIEVLGAPTEKLNLVNTRSLRVPFTRSISEPEPRCSIRFVRSWPTTGRSEESEQQGYFIRGIFSTLSNGFSRMLSLKGESTNEPVREDVKGPPTHRLSCGQSPYTETTTWERKYCILTDSQLVLLNKEKEIPVEGGQEQQTDSTKGRCLRRTVSVPSEGQFPEYPPEGATKLEVPAERSPRRRSISGTSTSEKPNSMDTVNTSPFKVPGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNADDRTRGLPKLKESRSHESLLSPCSAVECLDLGRGEPVSVKPLHSSILGQDFCFEVTYLSGSKCFSCNSASERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYSLPPLHSITVHIYKDVEKKKKKDKNNYVGLVNIPTASVTGRQFVEKWYPVSTPTPNKGKTGGPSIRIKSRFQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEELACALVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTIATKSIEEYLKLVGQQYLHDALGEFIKALYESDENCEVDPSKCSSSELIDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCLNRGKQDISERLISASLFLRFLCPAIMSPSLFNLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNTPGFDGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVAKLGPLPRVLADITKSLTNPTPIQQQLRRFTEHNSSPNVSGSLSSGLQKIFEDPTDSDLHKLKSPSQDNTDGYFRGKTLLLVQQASSQSMTYSEKDEKESSLPNGRSISLMDLQDTHAAQVEHASIMLDVPMRLTGSQLSITQVASIKQLRETQSTPQSAPQVRRPLHPALNQPGSLQPLSFQNPVYHLNNPIPAMPKASVDSSLENLSTASSRSQSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTVPDRHIPLALPRQNSTGQAQIRKMDQAGLGARAKAPPSLPHSASLRSTGSMSVASAALVAEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEDVEETEQNPDESKHAEKYEQEITKLKERLRVSSRRLEEYERRLLVQEQQMQKLLLEYKARLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQRSWE

Target ORF information:

RefSeq Version	XM_022421007.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022421007.1

ORF Insert Sequence:

ATGGAGTCGT GGAAGATGAG CATCTTTAAG AGACAGACAT CACCTTGTCC TCCAAGCTAT 
CGAAGATCTT TGTCAGAACC TGCTTATGCA AAATCAGAGA TAGGAATAAG CTTAAAGAGT 
TATCCATGGC GTGGTCCAGT GAAAATCAAG AGGAAGAAAA ATGAACATGC TGTTTCCAGT 
CTAGGAAAGC CACTGGGTGC TTATCGGTGG CAAACACATC CCAGCTGTAG GTGGAAAGGG 
GGCAAAAGGG AGAGGTTACA GACTAATAAA AGAGACTTGG ATGAACGGCA ACAAAAGGTC 
CTGCCAGAGA TGGTTGGTAG TAATGCTTCT TCCTCTGCTA TTGAGGTCCT TGGAGCACCA 
ACAGAGAAGC TGAACCTGGT CAACACCCGG TCACTTCGGG TTCCATTCAC CAGATCTATT 
TCAGAGCCAG AACCTCGCTG CAGTATCAGA TTTGTTAGAT CATGGCCGAC TACAGGAAGG 
TCAGAGGAGT CTGAACAGCA GGGATATTTC ATCCGTGGCA TTTTCTCTAC TCTTTCCAAT 
GGCTTCTCCA GGATGCTGAG TCTGAAAGGA GAATCAACCA ATGAGCCAGT AAGAGAAGAT 
GTGAAGGGGC CACCTACCCA TCGTCTGTCT TGTGGACAGT CGCCCTACAC AGAGACGACA 
ACATGGGAGC GGAAGTATTG CATCCTCACC GACAGCCAGT TGGTGTTGCT CAACAAGGAG 
AAGGAGATAC CTGTGGAGGG AGGACAGGAG CAGCAGACAG ATTCCACCAA AGGGCGATGC 
CTGAGGAGAA CCGTCAGTGT CCCTTCCGAG GGCCAGTTTC CTGAGTACCC ACCAGAGGGC 
GCCACCAAAC TGGAGGTGCC GGCAGAAAGG TCCCCACGCA GACGGAGTAT CTCAGGGACC 
AGTACATCAG AGAAACCCAA CTCCATGGAC ACTGTAAATA CCTCACCCTT CAAAGTACCA 
GGGTTCTTCA GCAAGCGCCT GAAAGGCTCC ATCAAGAGGA CCAAAAGCCA ATCAAAGCTT 
GACAGAAACA CGAGCTTTCG GCTACCATCC CTTCGTAATG CAGATGACAG GACTCGCGGT 
CTGCCTAAAC TAAAAGAGTC ACGTTCCCAT GAGTCATTGC TGAGCCCTTG TAGTGCAGTG 
GAATGTCTGG ATCTTGGCAG AGGAGAGCCT GTGTCAGTGA AGCCACTCCA TAGCAGCATC 
CTTGGACAAG ACTTCTGCTT TGAGGTTACG TACTTAAGTG GAAGTAAATG CTTCAGCTGT 
AACTCTGCTT CAGAGAGAGA TAAGTGGATG GAGAACCTTC GTAGGACAGT TCAACCAAAT 
AAGGACAATT GCAGACGAGC TGAAAACGTT CTTCGTTTAT GGATCATTGA AGCCAAGGAC 
CTTGCCCCCA AAAAGAAATA TTTCTGTGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT 
ACAACCAGCA AGACCAAAGC AGATAATATT TTCTGGGGTG AACATTTTGA ATTTTACAGC 
CTTCCACCTC TTCATAGCAT CACAGTTCAC ATTTATAAAG ATGTGGAAAA AAAGAAAAAA 
AAAGACAAGA ATAATTATGT AGGGCTAGTC AACATTCCCA CTGCCAGTGT GACTGGGCGC 
CAATTTGTAG AAAAGTGGTA TCCAGTGAGT ACCCCTACAC CCAACAAAGG AAAGACCGGA 
GGACCTTCTA TTCGGATTAA ATCACGTTTC CAAACTATCA CCATTCTGCC TATGGAGCAA 
TACAAAGAAT TTGCAGAATT TGTCACCAGC AACTATACCA TGCTGTGTTC TGTCCTTGAA 
CCAGTAATTA GTGTGAGAAA TAAAGAGGAG TTGGCCTGTG CCCTAGTGCA CATTCTTCAA 
AGTACTGGCA GAGCCAAGGA TTTTCTGACC GACTTGGTGA TGTCTGAGGT GGATCGCTGT 
GGAGAGCATG ACGTCTTGAT CTTCAGAGAG AACACCATTG CTACCAAATC CATTGAGGAA 
TACCTCAAGT TGGTGGGACA ACAGTATCTT CATGACGCAT TGGGGGAGTT CATCAAAGCT 
TTGTACGAGT CAGATGAGAA CTGTGAAGTG GATCCCAGCA AGTGTTCCTC TAGTGAACTG 
ATAGACCATC AGAGCAACCT GAAGATGTGC TGTGAGCTGG CTTTCTGCAA GATCATCAAC 
TCTTACTGTG TTTTCCCTCG TGAGTTGAAA GAAGTGTTTG CATCGTGGAA GCAGCAGTGC 
CTGAACCGTG GCAAACAGGA CATCAGCGAG CGGCTCATCA GTGCCTCATT GTTTCTCCGT 
TTCCTGTGCC CTGCCATCAT GTCTCCCAGT CTCTTCAACC TTATGCAAGA GTACCCTGAT 
GACCGTACAT CTCGCACCCT GACTCTGATT GCCAAGGTCA TTCAGAACCT GGCTAACTTT 
GCCAAGTTTG GTAACAAAGA AGAATATATG GCATTCATGA ATGATTTTTT AGAACATGAA 
TGGGGTGGAA TGAAGCGCTT TCTTTTGGAA ATCTCTAATC CGGACACCAT CTCAAACACC 
CCAGGCTTTG ATGGTTACAT TGATCTGGGC CGAGAGCTTT CAGTCTTGCA TTCCTTACTA 
TGGGAAGTAG TTTCCCAGCT TGATAAGGGT GAAAATTCCT TCCTACAGGC GACCGTGGCA 
AAATTGGGAC CGCTCCCTCG AGTTCTTGCT GATATTACCA AGTCTCTAAC TAATCCTACA 
CCAATACAAC AGCAGCTGAG ACGCTTCACT GAGCATAACT CCAGTCCAAA TGTCAGTGGA 
AGCCTCTCCT CTGGGCTGCA GAAAATATTT GAAGACCCCA CTGACAGTGA TTTGCATAAA 
CTAAAATCTC CAAGCCAGGA CAACACAGAT GGCTATTTCA GAGGGAAAAC GTTATTGCTG 
GTTCAGCAGG CCTCCTCCCA GAGCATGACC TATTCTGAAA AGGATGAAAA GGAAAGTAGC 
CTTCCTAACG GTCGGAGCAT CTCCCTCATG GACCTTCAAG ACACTCACGC TGCTCAAGTA 
GAGCATGCAT CTATCATGCT CGATGTGCCT ATGCGCTTAA CTGGAAGCCA GCTTTCCATA 
ACCCAGGTGG CCAGTATCAA ACAACTGCGG GAAACCCAGA GCACTCCCCA GAGTGCACCC 
CAAGTGCGAA GGCCCTTGCA CCCAGCCTTG AACCAGCCAG GCAGCCTCCA GCCCCTGTCA 
TTCCAGAACC CTGTCTATCA CCTCAATAAC CCAATCCCAG CAATGCCAAA GGCCTCTGTG 
GATTCTAGTT TGGAGAATCT AAGCACTGCC AGTTCCAGAA GCCAAAGTAA CAGTGAAGAT 
TTCAAACTCA GTGGGCCCAG CAATAGCAGC ATGGAAGATT TCACTAAACG TAGCACGCAG 
AGCGAGGATT TCTCCAGGCG GCACACTGTA CCAGACAGAC ACATACCTCT TGCTCTGCCG 
CGGCAGAATA GTACCGGGCA GGCCCAGATC CGGAAAATGG ACCAGGCTGG GTTAGGTGCC 
CGAGCCAAAG CCCCGCCGTC CTTGCCACAC AGTGCTTCCT TACGTAGCAC GGGGAGCATG 
TCGGTGGCCT CTGCGGCCCT GGTGGCTGAA CCTGTGCAGA ATGGGAGCCG GTCCCGGCAG 
CAGTCCTCTT CCTCCAGAGA GAGCCCCGTT CCCAAAGTGA GAGCGATCCA GAGACAACAG 
ACACAGCAGG TGCAGTCACC TGTGGACTCT GCCACCATGT CCCCAGTAGA GAGGACAGCA 
GCCTGGGTGT TGAACAATGG ACAGTATGAA GAGGATGTGG AAGAGACTGA GCAAAATCCA 
GATGAATCCA AGCATGCTGA GAAGTATGAG CAGGAAATTA CTAAACTGAA GGAGCGCCTG 
AGAGTGTCCA GCCGGCGCCT TGAGGAATAT GAGCGCCGCT TGCTGGTGCA GGAGCAGCAG 
ATGCAGAAGC TGCTGCTGGA ATACAAGGCC AGGCTGGAGG ACAGCGAGGA GCGCCTGCGC 
AGGCAGCAGG AGGAGAAGGA CAGCCAGATG AAAAGCATCA TCAGCAGGCT GATGGCTGTG 
GAAGAGGAAC TGAAGAAGGA TCATGCTGAG ATGCAAGCAG TTATTGATGC AAAGCAAAAA 
ATAATCGATG CACAGAGGAG TTGGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_022421008.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022276716.1 Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OCe289531
Clone ID Related Accession (Same CDS sequence)	XM_022421008.1
Accession Version	XM_022421008.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3735bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	ras GTPase-activating protein nGAP isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTTCT CCTTGAGTGA GGCGAGGCTG CAAACCGAGC TACTACGGAA TAGCTTAGCA 
GTGGATGTGA AGGGGCCACC TACCCATCGT CTGTCTTGTG GACAGTCGCC CTACACAGAG 
ACGACAACAT GGGAGCGGAA GTATTGCATC CTCACCGACA GCCAGTTGGT GTTGCTCAAC 
AAGGAGAAGG AGATACCTGT GGAGGGAGGA CAGGAGCAGC AGACAGATTC CACCAAAGGG 
CGATGCCTGA GGAGAACCGT CAGTGTCCCT TCCGAGGGCC AGTTTCCTGA GTACCCACCA 
GAGGGCGCCA CCAAACTGGA GGTGCCGGCA GAAAGGTCCC CACGCAGACG GAGTATCTCA 
GGGACCAGTA CATCAGAGAA ACCCAACTCC ATGGACACTG TAAATACCTC ACCCTTCAAA 
GTACCAGGGT TCTTCAGCAA GCGCCTGAAA GGCTCCATCA AGAGGACCAA AAGCCAATCA 
AAGCTTGACA GAAACACGAG CTTTCGGCTA CCATCCCTTC GTAATGCAGA TGACAGGACT 
CGCGGTCTGC CTAAACTAAA AGAGTCACGT TCCCATGAGT CATTGCTGAG CCCTTGTAGT 
GCAGTGGAAT GTCTGGATCT TGGCAGAGGA GAGCCTGTGT CAGTGAAGCC ACTCCATAGC 
AGCATCCTTG GACAAGACTT CTGCTTTGAG GTTACGTACT TAAGTGGAAG TAAATGCTTC 
AGCTGTAACT CTGCTTCAGA GAGAGATAAG TGGATGGAGA ACCTTCGTAG GACAGTTCAA 
CCAAATAAGG ACAATTGCAG ACGAGCTGAA AACGTTCTTC GTTTATGGAT CATTGAAGCC 
AAGGACCTTG CCCCCAAAAA GAAATATTTC TGTGAACTGT GCCTTGATGA TACCCTCTTT 
GCTCGTACAA CCAGCAAGAC CAAAGCAGAT AATATTTTCT GGGGTGAACA TTTTGAATTT 
TACAGCCTTC CACCTCTTCA TAGCATCACA GTTCACATTT ATAAAGATGT GGAAAAAAAG 
AAAAAAAAAG ACAAGAATAA TTATGTAGGG CTAGTCAACA TTCCCACTGC CAGTGTGACT 
GGGCGCCAAT TTGTAGAAAA GTGGTATCCA GTGAGTACCC CTACACCCAA CAAAGGAAAG 
ACCGGAGGAC CTTCTATTCG GATTAAATCA CGTTTCCAAA CTATCACCAT TCTGCCTATG 
GAGCAATACA AAGAATTTGC AGAATTTGTC ACCAGCAACT ATACCATGCT GTGTTCTGTC 
CTTGAACCAG TAATTAGTGT GAGAAATAAA GAGGAGTTGG CCTGTGCCCT AGTGCACATT 
CTTCAAAGTA CTGGCAGAGC CAAGGATTTT CTGACCGACT TGGTGATGTC TGAGGTGGAT 
CGCTGTGGAG AGCATGACGT CTTGATCTTC AGAGAGAACA CCATTGCTAC CAAATCCATT 
GAGGAATACC TCAAGTTGGT GGGACAACAG TATCTTCATG ACGCATTGGG GGAGTTCATC 
AAAGCTTTGT ACGAGTCAGA TGAGAACTGT GAAGTGGATC CCAGCAAGTG TTCCTCTAGT 
GAACTGATAG ACCATCAGAG CAACCTGAAG ATGTGCTGTG AGCTGGCTTT CTGCAAGATC 
ATCAACTCTT ACTGTGTTTT CCCTCGTGAG TTGAAAGAAG TGTTTGCATC GTGGAAGCAG 
CAGTGCCTGA ACCGTGGCAA ACAGGACATC AGCGAGCGGC TCATCAGTGC CTCATTGTTT 
CTCCGTTTCC TGTGCCCTGC CATCATGTCT CCCAGTCTCT TCAACCTTAT GCAAGAGTAC 
CCTGATGACC GTACATCTCG CACCCTGACT CTGATTGCCA AGGTCATTCA GAACCTGGCT 
AACTTTGCCA AGTTTGGTAA CAAAGAAGAA TATATGGCAT TCATGAATGA TTTTTTAGAA 
CATGAATGGG GTGGAATGAA GCGCTTTCTT TTGGAAATCT CTAATCCGGA CACCATCTCA 
AACACCCCAG GCTTTGATGG TTACATTGAT CTGGGCCGAG AGCTTTCAGT CTTGCATTCC 
TTACTATGGG AAGTAGTTTC CCAGCTTGAT AAGGGTGAAA ATTCCTTCCT ACAGGCGACC 
GTGGCAAAAT TGGGACCGCT CCCTCGAGTT CTTGCTGATA TTACCAAGTC TCTAACTAAT 
CCTACACCAA TACAACAGCA GCTGAGACGC TTCACTGAGC ATAACTCCAG TCCAAATGTC 
AGTGGAAGCC TCTCCTCTGG GCTGCAGAAA ATATTTGAAG ACCCCACTGA CAGTGATTTG 
CATAAACTAA AATCTCCAAG CCAGGACAAC ACAGATGGCT ATTTCAGAGG GAAAACGTTA 
TTGCTGGTTC AGCAGGCCTC CTCCCAGAGC ATGACCTATT CTGAAAAGGA TGAAAAGGAA 
AGTAGCCTTC CTAACGGTCG GAGCATCTCC CTCATGGACC TTCAAGACAC TCACGCTGCT 
CAAGTAGAGC ATGCATCTAT CATGCTCGAT GTGCCTATGC GCTTAACTGG AAGCCAGCTT 
TCCATAACCC AGGTGGCCAG TATCAAACAA CTGCGGGAAA CCCAGAGCAC TCCCCAGAGT 
GCACCCCAAG TGCGAAGGCC CTTGCACCCA GCCTTGAACC AGCCAGGCAG CCTCCAGCCC 
CTGTCATTCC AGAACCCTGT CTATCACCTC AATAACCCAA TCCCAGCAAT GCCAAAGGCC 
TCTGTGGATT CTAGTTTGGA GAATCTAAGC ACTGCCAGTT CCAGAAGCCA AAGTAACAGT 
GAAGATTTCA AACTCAGTGG GCCCAGCAAT AGCAGCATGG AAGATTTCAC TAAACGTAGC 
ACGCAGAGCG AGGATTTCTC CAGGCGGCAC ACTGTACCAG ACAGACACAT ACCTCTTGCT 
CTGCCGCGGC AGAATAGTAC CGGGCAGGCC CAGATCCGGA AAATGGACCA GGCTGGGTTA 
GGTGCCCGAG CCAAAGCCCC GCCGTCCTTG CCACACAGTG CTTCCTTACG TAGCACGGGG 
AGCATGTCGG TGGCCTCTGC GGCCCTGGTG GCTGAACCTG TGCAGAATGG GAGCCGGTCC 
CGGCAGCAGT CCTCTTCCTC CAGAGAGAGC CCCGTTCCCA AAGTGAGAGC GATCCAGAGA 
CAACAGACAC AGCAGGTGCA GTCACCTGTG GACTCTGCCA CCATGTCCCC AGTAGAGAGG 
ACAGCAGCCT GGGTGTTGAA CAATGGACAG TATGAAGAGG ATGTGGAAGA GACTGAGCAA 
AATCCAGATG AATCCAAGCA TGCTGAGAAG TATGAGCAGG AAATTACTAA ACTGAAGGAG 
CGCCTGAGAG TGTCCAGCCG GCGCCTTGAG GAATATGAGC GCCGCTTGCT GGTGCAGGAG 
CAGCAGATGC AGAAGCTGCT GCTGGAATAC AAGGCCAGGC TGGAGGACAG CGAGGAGCGC 
CTGCGCAGGC AGCAGGAGGA GAAGGACAGC CAGATGAAAA GCATCATCAG CAGGCTGATG 
GCTGTGGAAG AGGAACTGAA GAAGGATCAT GCTGAGATGC AAGCAGTTAT TGATGCAAAG 
CAAAAAATAA TCGATGCACA GGTCATAAAT GGAGATGAGA TTATTCAAAC AGGAAAAACG 
AATCGTGTCC CTGGATTCGG CCAACACCAG ACTGATGAGC GCACTGACCC AAGTGAAGGA 
GCGGTACAGC ATGCAGGTCC GCAATGGCAT CTCCCCCACC AACCCCACCA AGCTTTCCAT 
CACGGAGAAT GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022276716.1
CDS	109..3843
Translation	MSFSLSEARLQTELLRNSLAVDVKGPPTHRLSCGQSPYTETTTWERKYCILTDSQLVLLNKEKEIPVEGGQEQQTDSTKGRCLRRTVSVPSEGQFPEYPPEGATKLEVPAERSPRRRSISGTSTSEKPNSMDTVNTSPFKVPGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNADDRTRGLPKLKESRSHESLLSPCSAVECLDLGRGEPVSVKPLHSSILGQDFCFEVTYLSGSKCFSCNSASERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYSLPPLHSITVHIYKDVEKKKKKDKNNYVGLVNIPTASVTGRQFVEKWYPVSTPTPNKGKTGGPSIRIKSRFQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEELACALVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTIATKSIEEYLKLVGQQYLHDALGEFIKALYESDENCEVDPSKCSSSELIDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCLNRGKQDISERLISASLFLRFLCPAIMSPSLFNLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNTPGFDGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVAKLGPLPRVLADITKSLTNPTPIQQQLRRFTEHNSSPNVSGSLSSGLQKIFEDPTDSDLHKLKSPSQDNTDGYFRGKTLLLVQQASSQSMTYSEKDEKESSLPNGRSISLMDLQDTHAAQVEHASIMLDVPMRLTGSQLSITQVASIKQLRETQSTPQSAPQVRRPLHPALNQPGSLQPLSFQNPVYHLNNPIPAMPKASVDSSLENLSTASSRSQSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTVPDRHIPLALPRQNSTGQAQIRKMDQAGLGARAKAPPSLPHSASLRSTGSMSVASAALVAEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEDVEETEQNPDESKHAEKYEQEITKLKERLRVSSRRLEEYERRLLVQEQQMQKLLLEYKARLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQVINGDEIIQTGKTNRVPGFGQHQTDERTDPSEGAVQHAGPQWHLPHQPHQAFHHGEW

Target ORF information:

RefSeq Version	XM_022421008.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022421008.1

ORF Insert Sequence:

ATGTCTTTCT CCTTGAGTGA GGCGAGGCTG CAAACCGAGC TACTACGGAA TAGCTTAGCA 
GTGGATGTGA AGGGGCCACC TACCCATCGT CTGTCTTGTG GACAGTCGCC CTACACAGAG 
ACGACAACAT GGGAGCGGAA GTATTGCATC CTCACCGACA GCCAGTTGGT GTTGCTCAAC 
AAGGAGAAGG AGATACCTGT GGAGGGAGGA CAGGAGCAGC AGACAGATTC CACCAAAGGG 
CGATGCCTGA GGAGAACCGT CAGTGTCCCT TCCGAGGGCC AGTTTCCTGA GTACCCACCA 
GAGGGCGCCA CCAAACTGGA GGTGCCGGCA GAAAGGTCCC CACGCAGACG GAGTATCTCA 
GGGACCAGTA CATCAGAGAA ACCCAACTCC ATGGACACTG TAAATACCTC ACCCTTCAAA 
GTACCAGGGT TCTTCAGCAA GCGCCTGAAA GGCTCCATCA AGAGGACCAA AAGCCAATCA 
AAGCTTGACA GAAACACGAG CTTTCGGCTA CCATCCCTTC GTAATGCAGA TGACAGGACT 
CGCGGTCTGC CTAAACTAAA AGAGTCACGT TCCCATGAGT CATTGCTGAG CCCTTGTAGT 
GCAGTGGAAT GTCTGGATCT TGGCAGAGGA GAGCCTGTGT CAGTGAAGCC ACTCCATAGC 
AGCATCCTTG GACAAGACTT CTGCTTTGAG GTTACGTACT TAAGTGGAAG TAAATGCTTC 
AGCTGTAACT CTGCTTCAGA GAGAGATAAG TGGATGGAGA ACCTTCGTAG GACAGTTCAA 
CCAAATAAGG ACAATTGCAG ACGAGCTGAA AACGTTCTTC GTTTATGGAT CATTGAAGCC 
AAGGACCTTG CCCCCAAAAA GAAATATTTC TGTGAACTGT GCCTTGATGA TACCCTCTTT 
GCTCGTACAA CCAGCAAGAC CAAAGCAGAT AATATTTTCT GGGGTGAACA TTTTGAATTT 
TACAGCCTTC CACCTCTTCA TAGCATCACA GTTCACATTT ATAAAGATGT GGAAAAAAAG 
AAAAAAAAAG ACAAGAATAA TTATGTAGGG CTAGTCAACA TTCCCACTGC CAGTGTGACT 
GGGCGCCAAT TTGTAGAAAA GTGGTATCCA GTGAGTACCC CTACACCCAA CAAAGGAAAG 
ACCGGAGGAC CTTCTATTCG GATTAAATCA CGTTTCCAAA CTATCACCAT TCTGCCTATG 
GAGCAATACA AAGAATTTGC AGAATTTGTC ACCAGCAACT ATACCATGCT GTGTTCTGTC 
CTTGAACCAG TAATTAGTGT GAGAAATAAA GAGGAGTTGG CCTGTGCCCT AGTGCACATT 
CTTCAAAGTA CTGGCAGAGC CAAGGATTTT CTGACCGACT TGGTGATGTC TGAGGTGGAT 
CGCTGTGGAG AGCATGACGT CTTGATCTTC AGAGAGAACA CCATTGCTAC CAAATCCATT 
GAGGAATACC TCAAGTTGGT GGGACAACAG TATCTTCATG ACGCATTGGG GGAGTTCATC 
AAAGCTTTGT ACGAGTCAGA TGAGAACTGT GAAGTGGATC CCAGCAAGTG TTCCTCTAGT 
GAACTGATAG ACCATCAGAG CAACCTGAAG ATGTGCTGTG AGCTGGCTTT CTGCAAGATC 
ATCAACTCTT ACTGTGTTTT CCCTCGTGAG TTGAAAGAAG TGTTTGCATC GTGGAAGCAG 
CAGTGCCTGA ACCGTGGCAA ACAGGACATC AGCGAGCGGC TCATCAGTGC CTCATTGTTT 
CTCCGTTTCC TGTGCCCTGC CATCATGTCT CCCAGTCTCT TCAACCTTAT GCAAGAGTAC 
CCTGATGACC GTACATCTCG CACCCTGACT CTGATTGCCA AGGTCATTCA GAACCTGGCT 
AACTTTGCCA AGTTTGGTAA CAAAGAAGAA TATATGGCAT TCATGAATGA TTTTTTAGAA 
CATGAATGGG GTGGAATGAA GCGCTTTCTT TTGGAAATCT CTAATCCGGA CACCATCTCA 
AACACCCCAG GCTTTGATGG TTACATTGAT CTGGGCCGAG AGCTTTCAGT CTTGCATTCC 
TTACTATGGG AAGTAGTTTC CCAGCTTGAT AAGGGTGAAA ATTCCTTCCT ACAGGCGACC 
GTGGCAAAAT TGGGACCGCT CCCTCGAGTT CTTGCTGATA TTACCAAGTC TCTAACTAAT 
CCTACACCAA TACAACAGCA GCTGAGACGC TTCACTGAGC ATAACTCCAG TCCAAATGTC 
AGTGGAAGCC TCTCCTCTGG GCTGCAGAAA ATATTTGAAG ACCCCACTGA CAGTGATTTG 
CATAAACTAA AATCTCCAAG CCAGGACAAC ACAGATGGCT ATTTCAGAGG GAAAACGTTA 
TTGCTGGTTC AGCAGGCCTC CTCCCAGAGC ATGACCTATT CTGAAAAGGA TGAAAAGGAA 
AGTAGCCTTC CTAACGGTCG GAGCATCTCC CTCATGGACC TTCAAGACAC TCACGCTGCT 
CAAGTAGAGC ATGCATCTAT CATGCTCGAT GTGCCTATGC GCTTAACTGG AAGCCAGCTT 
TCCATAACCC AGGTGGCCAG TATCAAACAA CTGCGGGAAA CCCAGAGCAC TCCCCAGAGT 
GCACCCCAAG TGCGAAGGCC CTTGCACCCA GCCTTGAACC AGCCAGGCAG CCTCCAGCCC 
CTGTCATTCC AGAACCCTGT CTATCACCTC AATAACCCAA TCCCAGCAAT GCCAAAGGCC 
TCTGTGGATT CTAGTTTGGA GAATCTAAGC ACTGCCAGTT CCAGAAGCCA AAGTAACAGT 
GAAGATTTCA AACTCAGTGG GCCCAGCAAT AGCAGCATGG AAGATTTCAC TAAACGTAGC 
ACGCAGAGCG AGGATTTCTC CAGGCGGCAC ACTGTACCAG ACAGACACAT ACCTCTTGCT 
CTGCCGCGGC AGAATAGTAC CGGGCAGGCC CAGATCCGGA AAATGGACCA GGCTGGGTTA 
GGTGCCCGAG CCAAAGCCCC GCCGTCCTTG CCACACAGTG CTTCCTTACG TAGCACGGGG 
AGCATGTCGG TGGCCTCTGC GGCCCTGGTG GCTGAACCTG TGCAGAATGG GAGCCGGTCC 
CGGCAGCAGT CCTCTTCCTC CAGAGAGAGC CCCGTTCCCA AAGTGAGAGC GATCCAGAGA 
CAACAGACAC AGCAGGTGCA GTCACCTGTG GACTCTGCCA CCATGTCCCC AGTAGAGAGG 
ACAGCAGCCT GGGTGTTGAA CAATGGACAG TATGAAGAGG ATGTGGAAGA GACTGAGCAA 
AATCCAGATG AATCCAAGCA TGCTGAGAAG TATGAGCAGG AAATTACTAA ACTGAAGGAG 
CGCCTGAGAG TGTCCAGCCG GCGCCTTGAG GAATATGAGC GCCGCTTGCT GGTGCAGGAG 
CAGCAGATGC AGAAGCTGCT GCTGGAATAC AAGGCCAGGC TGGAGGACAG CGAGGAGCGC 
CTGCGCAGGC AGCAGGAGGA GAAGGACAGC CAGATGAAAA GCATCATCAG CAGGCTGATG 
GCTGTGGAAG AGGAACTGAA GAAGGATCAT GCTGAGATGC AAGCAGTTAT TGATGCAAAG 
CAAAAAATAA TCGATGCACA GGTCATAAAT GGAGATGAGA TTATTCAAAC AGGAAAAACG 
AATCGTGTCC CTGGATTCGG CCAACACCAG ACTGATGAGC GCACTGACCC AAGTGAAGGA 
GCGGTACAGC ATGCAGGTCC GCAATGGCAT CTCCCCCACC AACCCCACCA AGCTTTCCAT 
CACGGAGAAT GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_014115156.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013970631.2 Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OCe289532
Clone ID Related Accession (Same CDS sequence)	XM_014115156.2
Accession Version	XM_014115156.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	ras GTPase-activating protein nGAP isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014115156.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAGTAA TTGTGTTCTG TATGGAAAAG TTTTCTGGCT GGAGAATGAG TCTCCTTTCC 
CCCGGTGATG TGGCCGAGTG GCTGGATAGT AGCACGGAAG AGGAGAAGAG CCTTCTGTCA 
TTTCTGGAGG TGCCGGCAGA AAGGTCCCCA CGCAGACGGA GTATCTCAGG GACCAGTACA 
TCAGAGAAAC CCAACTCCAT GGACACTGTA AATACCTCAC CCTTCAAAGT ACCAGGGTTC 
TTCAGCAAGC GCCTGAAAGG CTCCATCAAG AGGACCAAAA GCCAATCAAA GCTTGACAGA 
AACACGAGCT TTCGGCTACC ATCCCTTCGT AATGCAGATG ACAGGACTCG CGGTCTGCCT 
AAACTAAAAG AGTCACGTTC CCATGAGTCA TTGCTGAGCC CTTGTAGTGC AGTGGAATGT 
CTGGATCTTG GCAGAGGAGA GCCTGTGTCA GTGAAGCCAC TCCATAGCAG CATCCTTGGA 
CAAGACTTCT GCTTTGAGGT TACGTACTTA AGTGGAAGTA AATGCTTCAG CTGTAACTCT 
GCTTCAGAGA GAGATAAGTG GATGGAGAAC CTTCGTAGGA CAGTTCAACC AAATAAGGAC 
AATTGCAGAC GAGCTGAAAA CGTTCTTCGT TTATGGATCA TTGAAGCCAA GGACCTTGCC 
CCCAAAAAGA AATATTTCTG TGAACTGTGC CTTGATGATA CCCTCTTTGC TCGTACAACC 
AGCAAGACCA AAGCAGATAA TATTTTCTGG GGTGAACATT TTGAATTTTA CAGCCTTCCA 
CCTCTTCATA GCATCACAGT TCACATTTAT AAAGATGTGG AAAAAAAGAA AAAAAAAGAC 
AAGAATAATT ATGTAGGGCT AGTCAACATT CCCACTGCCA GTGTGACTGG GCGCCAATTT 
GTAGAAAAGT GGTATCCAGT GAGTACCCCT ACACCCAACA AAGGAAAGAC CGGAGGACCT 
TCTATTCGGA TTAAATCACG TTTCCAAACT ATCACCATTC TGCCTATGGA GCAATACAAA 
GAATTTGCAG AATTTGTCAC CAGCAACTAT ACCATGCTGT GTTCTGTCCT TGAACCAGTA 
ATTAGTGTGA GAAATAAAGA GGAGTTGGCC TGTGCCCTAG TGCACATTCT TCAAAGTACT 
GGCAGAGCCA AGGATTTTCT GACCGACTTG GTGATGTCTG AGGTGGATCG CTGTGGAGAG 
CATGACGTCT TGATCTTCAG AGAGAACACC ATTGCTACCA AATCCATTGA GGAATACCTC 
AAGTTGGTGG GACAACAGTA TCTTCATGAC GCATTGGGGG AGTTCATCAA AGCTTTGTAC 
GAGTCAGATG AGAACTGTGA AGTGGATCCC AGCAAGTGTT CCTCTAGTGA ACTGATAGAC 
CATCAGAGCA ACCTGAAGAT GTGCTGTGAG CTGGCTTTCT GCAAGATCAT CAACTCTTAC 
TGTGTTTTCC CTCGTGAGTT GAAAGAAGTG TTTGCATCGT GGAAGCAGCA GTGCCTGAAC 
CGTGGCAAAC AGGACATCAG CGAGCGGCTC ATCAGTGCCT CATTGTTTCT CCGTTTCCTG 
TGCCCTGCCA TCATGTCTCC CAGTCTCTTC AACCTTATGC AAGAGTACCC TGATGACCGT 
ACATCTCGCA CCCTGACTCT GATTGCCAAG GTCATTCAGA ACCTGGCTAA CTTTGCCAAG 
TTTGGTAACA AAGAAGAATA TATGGCATTC ATGAATGATT TTTTAGAACA TGAATGGGGT 
GGAATGAAGC GCTTTCTTTT GGAAATCTCT AATCCGGACA CCATCTCAAA CACCCCAGGC 
TTTGATGGTT ACATTGATCT GGGCCGAGAG CTTTCAGTCT TGCATTCCTT ACTATGGGAA 
GTAGTTTCCC AGCTTGATAA GGGTGAAAAT TCCTTCCTAC AGGCGACCGT GGCAAAATTG 
GGACCGCTCC CTCGAGTTCT TGCTGATATT ACCAAGTCTC TAACTAATCC TACACCAATA 
CAACAGCAGC TGAGACGCTT CACTGAGCAT AACTCCAGTC CAAATGTCAG TGGAAGCCTC 
TCCTCTGGGC TGCAGAAAAT ATTTGAAGAC CCCACTGACA GTGATTTGCA TAAACTAAAA 
TCTCCAAGCC AGGACAACAC AGATGGCTAT TTCAGAGGGA AAACGTTATT GCTGGTTCAG 
CAGGCCTCCT CCCAGAGCAT GACCTATTCT GAAAAGGATG AAAAGGAAAG TAGCCTTCCT 
AACGGTCGGA GCATCTCCCT CATGGACCTT CAAGACACTC ACGCTGCTCA AGTAGAGCAT 
GCATCTATCA TGCTCGATGT GCCTATGCGC TTAACTGGAA GCCAGCTTTC CATAACCCAG 
GTGGCCAGTA TCAAACAACT GCGGGAAACC CAGAGCACTC CCCAGAGTGC ACCCCAAGTG 
CGAAGGCCCT TGCACCCAGC CTTGAACCAG CCAGGCAGCC TCCAGCCCCT GTCATTCCAG 
AACCCTGTCT ATCACCTCAA TAACCCAATC CCAGCAATGC CAAAGGCCTC TGTGGATTCT 
AGTTTGGAGA ATCTAAGCAC TGCCAGTTCC AGAAGCCAAA GTAACAGTGA AGATTTCAAA 
CTCAGTGGGC CCAGCAATAG CAGCATGGAA GATTTCACTA AACGTAGCAC GCAGAGCGAG 
GATTTCTCCA GGCGGCACAC TGTACCAGAC AGACACATAC CTCTTGCTCT GCCGCGGCAG 
AATAGTACCG GGCAGGCCCA GATCCGGAAA ATGGACCAGG CTGGGTTAGG TGCCCGAGCC 
AAAGCCCCGC CGTCCTTGCC ACACAGTGCT TCCTTACGTA GCACGGGGAG CATGTCGGTG 
GCCTCTGCGG CCCTGGTGGC TGAACCTGTG CAGAATGGGA GCCGGTCCCG GCAGCAGTCC 
TCTTCCTCCA GAGAGAGCCC CGTTCCCAAA GTGAGAGCGA TCCAGAGACA ACAGACACAG 
CAGGTGCAGT CACCTGTGGA CTCTGCCACC ATGTCCCCAG TAGAGAGGAC AGCAGCCTGG 
GTGTTGAACA ATGGACAGTA TGAAGAGGAT GTGGAAGAGA CTGAGCAAAA TCCAGATGAA 
TCCAAGCATG CTGAGAAGTA TGAGCAGGAA ATTACTAAAC TGAAGGAGCG CCTGAGAGTG 
TCCAGCCGGC GCCTTGAGGA ATATGAGCGC CGCTTGCTGG TGCAGGAGCA GCAGATGCAG 
AAGCTGCTGC TGGAATACAA GGCCAGGCTG GAGGACAGCG AGGAGCGCCT GCGCAGGCAG 
CAGGAGGAGA AGGACAGCCA GATGAAAAGC ATCATCAGCA GGCTGATGGC TGTGGAAGAG 
GAACTGAAGA AGGATCATGC TGAGATGCAA GCAGTTATTG ATGCAAAGCA AAAAATAATC 
GATGCACAGG TCATAAATGG AGATGAGATT ATTCAAACAG GAAAAACGAA TCGTGTCCCT 
GGATTCGGCC AACACCAGAC TGATGAGCGC ACTGACCCAA GTGAAGGAGC GGTACAGCAT 
GCAGGTCCGC AATGGCATCT CCCCCACCAA CCCCACCAAG CTTTCCATCA CGGAGAATGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013970631.2
CDS	16..3558
Translation	MRVIVFCMEKFSGWRMSLLSPGDVAEWLDSSTEEEKSLLSFLEVPAERSPRRRSISGTSTSEKPNSMDTVNTSPFKVPGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNADDRTRGLPKLKESRSHESLLSPCSAVECLDLGRGEPVSVKPLHSSILGQDFCFEVTYLSGSKCFSCNSASERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYSLPPLHSITVHIYKDVEKKKKKDKNNYVGLVNIPTASVTGRQFVEKWYPVSTPTPNKGKTGGPSIRIKSRFQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEELACALVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTIATKSIEEYLKLVGQQYLHDALGEFIKALYESDENCEVDPSKCSSSELIDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCLNRGKQDISERLISASLFLRFLCPAIMSPSLFNLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNTPGFDGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVAKLGPLPRVLADITKSLTNPTPIQQQLRRFTEHNSSPNVSGSLSSGLQKIFEDPTDSDLHKLKSPSQDNTDGYFRGKTLLLVQQASSQSMTYSEKDEKESSLPNGRSISLMDLQDTHAAQVEHASIMLDVPMRLTGSQLSITQVASIKQLRETQSTPQSAPQVRRPLHPALNQPGSLQPLSFQNPVYHLNNPIPAMPKASVDSSLENLSTASSRSQSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTVPDRHIPLALPRQNSTGQAQIRKMDQAGLGARAKAPPSLPHSASLRSTGSMSVASAALVAEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEDVEETEQNPDESKHAEKYEQEITKLKERLRVSSRRLEEYERRLLVQEQQMQKLLLEYKARLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQVINGDEIIQTGKTNRVPGFGQHQTDERTDPSEGAVQHAGPQWHLPHQPHQAFHHGEW

Target ORF information:

RefSeq Version	XM_014115156.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014115156.2

ORF Insert Sequence:

ATGCGAGTAA TTGTGTTCTG TATGGAAAAG TTTTCTGGCT GGAGAATGAG TCTCCTTTCC 
CCCGGTGATG TGGCCGAGTG GCTGGATAGT AGCACGGAAG AGGAGAAGAG CCTTCTGTCA 
TTTCTGGAGG TGCCGGCAGA AAGGTCCCCA CGCAGACGGA GTATCTCAGG GACCAGTACA 
TCAGAGAAAC CCAACTCCAT GGACACTGTA AATACCTCAC CCTTCAAAGT ACCAGGGTTC 
TTCAGCAAGC GCCTGAAAGG CTCCATCAAG AGGACCAAAA GCCAATCAAA GCTTGACAGA 
AACACGAGCT TTCGGCTACC ATCCCTTCGT AATGCAGATG ACAGGACTCG CGGTCTGCCT 
AAACTAAAAG AGTCACGTTC CCATGAGTCA TTGCTGAGCC CTTGTAGTGC AGTGGAATGT 
CTGGATCTTG GCAGAGGAGA GCCTGTGTCA GTGAAGCCAC TCCATAGCAG CATCCTTGGA 
CAAGACTTCT GCTTTGAGGT TACGTACTTA AGTGGAAGTA AATGCTTCAG CTGTAACTCT 
GCTTCAGAGA GAGATAAGTG GATGGAGAAC CTTCGTAGGA CAGTTCAACC AAATAAGGAC 
AATTGCAGAC GAGCTGAAAA CGTTCTTCGT TTATGGATCA TTGAAGCCAA GGACCTTGCC 
CCCAAAAAGA AATATTTCTG TGAACTGTGC CTTGATGATA CCCTCTTTGC TCGTACAACC 
AGCAAGACCA AAGCAGATAA TATTTTCTGG GGTGAACATT TTGAATTTTA CAGCCTTCCA 
CCTCTTCATA GCATCACAGT TCACATTTAT AAAGATGTGG AAAAAAAGAA AAAAAAAGAC 
AAGAATAATT ATGTAGGGCT AGTCAACATT CCCACTGCCA GTGTGACTGG GCGCCAATTT 
GTAGAAAAGT GGTATCCAGT GAGTACCCCT ACACCCAACA AAGGAAAGAC CGGAGGACCT 
TCTATTCGGA TTAAATCACG TTTCCAAACT ATCACCATTC TGCCTATGGA GCAATACAAA 
GAATTTGCAG AATTTGTCAC CAGCAACTAT ACCATGCTGT GTTCTGTCCT TGAACCAGTA 
ATTAGTGTGA GAAATAAAGA GGAGTTGGCC TGTGCCCTAG TGCACATTCT TCAAAGTACT 
GGCAGAGCCA AGGATTTTCT GACCGACTTG GTGATGTCTG AGGTGGATCG CTGTGGAGAG 
CATGACGTCT TGATCTTCAG AGAGAACACC ATTGCTACCA AATCCATTGA GGAATACCTC 
AAGTTGGTGG GACAACAGTA TCTTCATGAC GCATTGGGGG AGTTCATCAA AGCTTTGTAC 
GAGTCAGATG AGAACTGTGA AGTGGATCCC AGCAAGTGTT CCTCTAGTGA ACTGATAGAC 
CATCAGAGCA ACCTGAAGAT GTGCTGTGAG CTGGCTTTCT GCAAGATCAT CAACTCTTAC 
TGTGTTTTCC CTCGTGAGTT GAAAGAAGTG TTTGCATCGT GGAAGCAGCA GTGCCTGAAC 
CGTGGCAAAC AGGACATCAG CGAGCGGCTC ATCAGTGCCT CATTGTTTCT CCGTTTCCTG 
TGCCCTGCCA TCATGTCTCC CAGTCTCTTC AACCTTATGC AAGAGTACCC TGATGACCGT 
ACATCTCGCA CCCTGACTCT GATTGCCAAG GTCATTCAGA ACCTGGCTAA CTTTGCCAAG 
TTTGGTAACA AAGAAGAATA TATGGCATTC ATGAATGATT TTTTAGAACA TGAATGGGGT 
GGAATGAAGC GCTTTCTTTT GGAAATCTCT AATCCGGACA CCATCTCAAA CACCCCAGGC 
TTTGATGGTT ACATTGATCT GGGCCGAGAG CTTTCAGTCT TGCATTCCTT ACTATGGGAA 
GTAGTTTCCC AGCTTGATAA GGGTGAAAAT TCCTTCCTAC AGGCGACCGT GGCAAAATTG 
GGACCGCTCC CTCGAGTTCT TGCTGATATT ACCAAGTCTC TAACTAATCC TACACCAATA 
CAACAGCAGC TGAGACGCTT CACTGAGCAT AACTCCAGTC CAAATGTCAG TGGAAGCCTC 
TCCTCTGGGC TGCAGAAAAT ATTTGAAGAC CCCACTGACA GTGATTTGCA TAAACTAAAA 
TCTCCAAGCC AGGACAACAC AGATGGCTAT TTCAGAGGGA AAACGTTATT GCTGGTTCAG 
CAGGCCTCCT CCCAGAGCAT GACCTATTCT GAAAAGGATG AAAAGGAAAG TAGCCTTCCT 
AACGGTCGGA GCATCTCCCT CATGGACCTT CAAGACACTC ACGCTGCTCA AGTAGAGCAT 
GCATCTATCA TGCTCGATGT GCCTATGCGC TTAACTGGAA GCCAGCTTTC CATAACCCAG 
GTGGCCAGTA TCAAACAACT GCGGGAAACC CAGAGCACTC CCCAGAGTGC ACCCCAAGTG 
CGAAGGCCCT TGCACCCAGC CTTGAACCAG CCAGGCAGCC TCCAGCCCCT GTCATTCCAG 
AACCCTGTCT ATCACCTCAA TAACCCAATC CCAGCAATGC CAAAGGCCTC TGTGGATTCT 
AGTTTGGAGA ATCTAAGCAC TGCCAGTTCC AGAAGCCAAA GTAACAGTGA AGATTTCAAA 
CTCAGTGGGC CCAGCAATAG CAGCATGGAA GATTTCACTA AACGTAGCAC GCAGAGCGAG 
GATTTCTCCA GGCGGCACAC TGTACCAGAC AGACACATAC CTCTTGCTCT GCCGCGGCAG 
AATAGTACCG GGCAGGCCCA GATCCGGAAA ATGGACCAGG CTGGGTTAGG TGCCCGAGCC 
AAAGCCCCGC CGTCCTTGCC ACACAGTGCT TCCTTACGTA GCACGGGGAG CATGTCGGTG 
GCCTCTGCGG CCCTGGTGGC TGAACCTGTG CAGAATGGGA GCCGGTCCCG GCAGCAGTCC 
TCTTCCTCCA GAGAGAGCCC CGTTCCCAAA GTGAGAGCGA TCCAGAGACA ACAGACACAG 
CAGGTGCAGT CACCTGTGGA CTCTGCCACC ATGTCCCCAG TAGAGAGGAC AGCAGCCTGG 
GTGTTGAACA ATGGACAGTA TGAAGAGGAT GTGGAAGAGA CTGAGCAAAA TCCAGATGAA 
TCCAAGCATG CTGAGAAGTA TGAGCAGGAA ATTACTAAAC TGAAGGAGCG CCTGAGAGTG 
TCCAGCCGGC GCCTTGAGGA ATATGAGCGC CGCTTGCTGG TGCAGGAGCA GCAGATGCAG 
AAGCTGCTGC TGGAATACAA GGCCAGGCTG GAGGACAGCG AGGAGCGCCT GCGCAGGCAG 
CAGGAGGAGA AGGACAGCCA GATGAAAAGC ATCATCAGCA GGCTGATGGC TGTGGAAGAG 
GAACTGAAGA AGGATCATGC TGAGATGCAA GCAGTTATTG ATGCAAAGCA AAAAATAATC 
GATGCACAGG TCATAAATGG AGATGAGATT ATTCAAACAG GAAAAACGAA TCGTGTCCCT 
GGATTCGGCC AACACCAGAC TGATGAGCGC ACTGACCCAA GTGAAGGAGC GGTACAGCAT 
GCAGGTCCGC AATGGCATCT CCCCCACCAA CCCCACCAAG CTTTCCATCA CGGAGAATGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_022421009.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022276717.1 Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OCe289533
Clone ID Related Accession (Same CDS sequence)	XM_022421009.1
Accession Version	XM_022421009.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3489bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	ras GTPase-activating protein nGAP isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAGGA AGAGAGCAAC CAGACAAAAT AATAACAATA ATACATCGAA AGCTGAAAAG 
CAGACGGAAT GTGAGGTGCC GGCAGAAAGG TCCCCACGCA GACGGAGTAT CTCAGGGACC 
AGTACATCAG AGAAACCCAA CTCCATGGAC ACTGTAAATA CCTCACCCTT CAAAGTACCA 
GGGTTCTTCA GCAAGCGCCT GAAAGGCTCC ATCAAGAGGA CCAAAAGCCA ATCAAAGCTT 
GACAGAAACA CGAGCTTTCG GCTACCATCC CTTCGTAATG CAGATGACAG GACTCGCGGT 
CTGCCTAAAC TAAAAGAGTC ACGTTCCCAT GAGTCATTGC TGAGCCCTTG TAGTGCAGTG 
GAATGTCTGG ATCTTGGCAG AGGAGAGCCT GTGTCAGTGA AGCCACTCCA TAGCAGCATC 
CTTGGACAAG ACTTCTGCTT TGAGGTTACG TACTTAAGTG GAAGTAAATG CTTCAGCTGT 
AACTCTGCTT CAGAGAGAGA TAAGTGGATG GAGAACCTTC GTAGGACAGT TCAACCAAAT 
AAGGACAATT GCAGACGAGC TGAAAACGTT CTTCGTTTAT GGATCATTGA AGCCAAGGAC 
CTTGCCCCCA AAAAGAAATA TTTCTGTGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT 
ACAACCAGCA AGACCAAAGC AGATAATATT TTCTGGGGTG AACATTTTGA ATTTTACAGC 
CTTCCACCTC TTCATAGCAT CACAGTTCAC ATTTATAAAG ATGTGGAAAA AAAGAAAAAA 
AAAGACAAGA ATAATTATGT AGGGCTAGTC AACATTCCCA CTGCCAGTGT GACTGGGCGC 
CAATTTGTAG AAAAGTGGTA TCCAGTGAGT ACCCCTACAC CCAACAAAGG AAAGACCGGA 
GGACCTTCTA TTCGGATTAA ATCACGTTTC CAAACTATCA CCATTCTGCC TATGGAGCAA 
TACAAAGAAT TTGCAGAATT TGTCACCAGC AACTATACCA TGCTGTGTTC TGTCCTTGAA 
CCAGTAATTA GTGTGAGAAA TAAAGAGGAG TTGGCCTGTG CCCTAGTGCA CATTCTTCAA 
AGTACTGGCA GAGCCAAGGA TTTTCTGACC GACTTGGTGA TGTCTGAGGT GGATCGCTGT 
GGAGAGCATG ACGTCTTGAT CTTCAGAGAG AACACCATTG CTACCAAATC CATTGAGGAA 
TACCTCAAGT TGGTGGGACA ACAGTATCTT CATGACGCAT TGGGGGAGTT CATCAAAGCT 
TTGTACGAGT CAGATGAGAA CTGTGAAGTG GATCCCAGCA AGTGTTCCTC TAGTGAACTG 
ATAGACCATC AGAGCAACCT GAAGATGTGC TGTGAGCTGG CTTTCTGCAA GATCATCAAC 
TCTTACTGTG TTTTCCCTCG TGAGTTGAAA GAAGTGTTTG CATCGTGGAA GCAGCAGTGC 
CTGAACCGTG GCAAACAGGA CATCAGCGAG CGGCTCATCA GTGCCTCATT GTTTCTCCGT 
TTCCTGTGCC CTGCCATCAT GTCTCCCAGT CTCTTCAACC TTATGCAAGA GTACCCTGAT 
GACCGTACAT CTCGCACCCT GACTCTGATT GCCAAGGTCA TTCAGAACCT GGCTAACTTT 
GCCAAGTTTG GTAACAAAGA AGAATATATG GCATTCATGA ATGATTTTTT AGAACATGAA 
TGGGGTGGAA TGAAGCGCTT TCTTTTGGAA ATCTCTAATC CGGACACCAT CTCAAACACC 
CCAGGCTTTG ATGGTTACAT TGATCTGGGC CGAGAGCTTT CAGTCTTGCA TTCCTTACTA 
TGGGAAGTAG TTTCCCAGCT TGATAAGGGT GAAAATTCCT TCCTACAGGC GACCGTGGCA 
AAATTGGGAC CGCTCCCTCG AGTTCTTGCT GATATTACCA AGTCTCTAAC TAATCCTACA 
CCAATACAAC AGCAGCTGAG ACGCTTCACT GAGCATAACT CCAGTCCAAA TGTCAGTGGA 
AGCCTCTCCT CTGGGCTGCA GAAAATATTT GAAGACCCCA CTGACAGTGA TTTGCATAAA 
CTAAAATCTC CAAGCCAGGA CAACACAGAT GGCTATTTCA GAGGGAAAAC GTTATTGCTG 
GTTCAGCAGG CCTCCTCCCA GAGCATGACC TATTCTGAAA AGGATGAAAA GGAAAGTAGC 
CTTCCTAACG GTCGGAGCAT CTCCCTCATG GACCTTCAAG ACACTCACGC TGCTCAAGTA 
GAGCATGCAT CTATCATGCT CGATGTGCCT ATGCGCTTAA CTGGAAGCCA GCTTTCCATA 
ACCCAGGTGG CCAGTATCAA ACAACTGCGG GAAACCCAGA GCACTCCCCA GAGTGCACCC 
CAAGTGCGAA GGCCCTTGCA CCCAGCCTTG AACCAGCCAG GCAGCCTCCA GCCCCTGTCA 
TTCCAGAACC CTGTCTATCA CCTCAATAAC CCAATCCCAG CAATGCCAAA GGCCTCTGTG 
GATTCTAGTT TGGAGAATCT AAGCACTGCC AGTTCCAGAA GCCAAAGTAA CAGTGAAGAT 
TTCAAACTCA GTGGGCCCAG CAATAGCAGC ATGGAAGATT TCACTAAACG TAGCACGCAG 
AGCGAGGATT TCTCCAGGCG GCACACTGTA CCAGACAGAC ACATACCTCT TGCTCTGCCG 
CGGCAGAATA GTACCGGGCA GGCCCAGATC CGGAAAATGG ACCAGGCTGG GTTAGGTGCC 
CGAGCCAAAG CCCCGCCGTC CTTGCCACAC AGTGCTTCCT TACGTAGCAC GGGGAGCATG 
TCGGTGGCCT CTGCGGCCCT GGTGGCTGAA CCTGTGCAGA ATGGGAGCCG GTCCCGGCAG 
CAGTCCTCTT CCTCCAGAGA GAGCCCCGTT CCCAAAGTGA GAGCGATCCA GAGACAACAG 
ACACAGCAGG TGCAGTCACC TGTGGACTCT GCCACCATGT CCCCAGTAGA GAGGACAGCA 
GCCTGGGTGT TGAACAATGG ACAGTATGAA GAGGATGTGG AAGAGACTGA GCAAAATCCA 
GATGAATCCA AGCATGCTGA GAAGTATGAG CAGGAAATTA CTAAACTGAA GGAGCGCCTG 
AGAGTGTCCA GCCGGCGCCT TGAGGAATAT GAGCGCCGCT TGCTGGTGCA GGAGCAGCAG 
ATGCAGAAGC TGCTGCTGGA ATACAAGGCC AGGCTGGAGG ACAGCGAGGA GCGCCTGCGC 
AGGCAGCAGG AGGAGAAGGA CAGCCAGATG AAAAGCATCA TCAGCAGGCT GATGGCTGTG 
GAAGAGGAAC TGAAGAAGGA TCATGCTGAG ATGCAAGCAG TTATTGATGC AAAGCAAAAA 
ATAATCGATG CACAGGTCAT AAATGGAGAT GAGATTATTC AAACAGGAAA AACGAATCGT 
GTCCCTGGAT TCGGCCAACA CCAGACTGAT GAGCGCACTG ACCCAAGTGA AGGAGCGGTA 
CAGCATGCAG GTCCGCAATG GCATCTCCCC CACCAACCCC ACCAAGCTTT CCATCACGGA 
GAATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022276717.1
CDS	16..3504
Translation	MGRKRATRQNNNNNTSKAEKQTECEVPAERSPRRRSISGTSTSEKPNSMDTVNTSPFKVPGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNADDRTRGLPKLKESRSHESLLSPCSAVECLDLGRGEPVSVKPLHSSILGQDFCFEVTYLSGSKCFSCNSASERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYSLPPLHSITVHIYKDVEKKKKKDKNNYVGLVNIPTASVTGRQFVEKWYPVSTPTPNKGKTGGPSIRIKSRFQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEELACALVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTIATKSIEEYLKLVGQQYLHDALGEFIKALYESDENCEVDPSKCSSSELIDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCLNRGKQDISERLISASLFLRFLCPAIMSPSLFNLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNTPGFDGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVAKLGPLPRVLADITKSLTNPTPIQQQLRRFTEHNSSPNVSGSLSSGLQKIFEDPTDSDLHKLKSPSQDNTDGYFRGKTLLLVQQASSQSMTYSEKDEKESSLPNGRSISLMDLQDTHAAQVEHASIMLDVPMRLTGSQLSITQVASIKQLRETQSTPQSAPQVRRPLHPALNQPGSLQPLSFQNPVYHLNNPIPAMPKASVDSSLENLSTASSRSQSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTVPDRHIPLALPRQNSTGQAQIRKMDQAGLGARAKAPPSLPHSASLRSTGSMSVASAALVAEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEDVEETEQNPDESKHAEKYEQEITKLKERLRVSSRRLEEYERRLLVQEQQMQKLLLEYKARLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQVINGDEIIQTGKTNRVPGFGQHQTDERTDPSEGAVQHAGPQWHLPHQPHQAFHHGEW

Target ORF information:

RefSeq Version	XM_022421009.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022421009.1

ORF Insert Sequence:

ATGGGAAGGA AGAGAGCAAC CAGACAAAAT AATAACAATA ATACATCGAA AGCTGAAAAG 
CAGACGGAAT GTGAGGTGCC GGCAGAAAGG TCCCCACGCA GACGGAGTAT CTCAGGGACC 
AGTACATCAG AGAAACCCAA CTCCATGGAC ACTGTAAATA CCTCACCCTT CAAAGTACCA 
GGGTTCTTCA GCAAGCGCCT GAAAGGCTCC ATCAAGAGGA CCAAAAGCCA ATCAAAGCTT 
GACAGAAACA CGAGCTTTCG GCTACCATCC CTTCGTAATG CAGATGACAG GACTCGCGGT 
CTGCCTAAAC TAAAAGAGTC ACGTTCCCAT GAGTCATTGC TGAGCCCTTG TAGTGCAGTG 
GAATGTCTGG ATCTTGGCAG AGGAGAGCCT GTGTCAGTGA AGCCACTCCA TAGCAGCATC 
CTTGGACAAG ACTTCTGCTT TGAGGTTACG TACTTAAGTG GAAGTAAATG CTTCAGCTGT 
AACTCTGCTT CAGAGAGAGA TAAGTGGATG GAGAACCTTC GTAGGACAGT TCAACCAAAT 
AAGGACAATT GCAGACGAGC TGAAAACGTT CTTCGTTTAT GGATCATTGA AGCCAAGGAC 
CTTGCCCCCA AAAAGAAATA TTTCTGTGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT 
ACAACCAGCA AGACCAAAGC AGATAATATT TTCTGGGGTG AACATTTTGA ATTTTACAGC 
CTTCCACCTC TTCATAGCAT CACAGTTCAC ATTTATAAAG ATGTGGAAAA AAAGAAAAAA 
AAAGACAAGA ATAATTATGT AGGGCTAGTC AACATTCCCA CTGCCAGTGT GACTGGGCGC 
CAATTTGTAG AAAAGTGGTA TCCAGTGAGT ACCCCTACAC CCAACAAAGG AAAGACCGGA 
GGACCTTCTA TTCGGATTAA ATCACGTTTC CAAACTATCA CCATTCTGCC TATGGAGCAA 
TACAAAGAAT TTGCAGAATT TGTCACCAGC AACTATACCA TGCTGTGTTC TGTCCTTGAA 
CCAGTAATTA GTGTGAGAAA TAAAGAGGAG TTGGCCTGTG CCCTAGTGCA CATTCTTCAA 
AGTACTGGCA GAGCCAAGGA TTTTCTGACC GACTTGGTGA TGTCTGAGGT GGATCGCTGT 
GGAGAGCATG ACGTCTTGAT CTTCAGAGAG AACACCATTG CTACCAAATC CATTGAGGAA 
TACCTCAAGT TGGTGGGACA ACAGTATCTT CATGACGCAT TGGGGGAGTT CATCAAAGCT 
TTGTACGAGT CAGATGAGAA CTGTGAAGTG GATCCCAGCA AGTGTTCCTC TAGTGAACTG 
ATAGACCATC AGAGCAACCT GAAGATGTGC TGTGAGCTGG CTTTCTGCAA GATCATCAAC 
TCTTACTGTG TTTTCCCTCG TGAGTTGAAA GAAGTGTTTG CATCGTGGAA GCAGCAGTGC 
CTGAACCGTG GCAAACAGGA CATCAGCGAG CGGCTCATCA GTGCCTCATT GTTTCTCCGT 
TTCCTGTGCC CTGCCATCAT GTCTCCCAGT CTCTTCAACC TTATGCAAGA GTACCCTGAT 
GACCGTACAT CTCGCACCCT GACTCTGATT GCCAAGGTCA TTCAGAACCT GGCTAACTTT 
GCCAAGTTTG GTAACAAAGA AGAATATATG GCATTCATGA ATGATTTTTT AGAACATGAA 
TGGGGTGGAA TGAAGCGCTT TCTTTTGGAA ATCTCTAATC CGGACACCAT CTCAAACACC 
CCAGGCTTTG ATGGTTACAT TGATCTGGGC CGAGAGCTTT CAGTCTTGCA TTCCTTACTA 
TGGGAAGTAG TTTCCCAGCT TGATAAGGGT GAAAATTCCT TCCTACAGGC GACCGTGGCA 
AAATTGGGAC CGCTCCCTCG AGTTCTTGCT GATATTACCA AGTCTCTAAC TAATCCTACA 
CCAATACAAC AGCAGCTGAG ACGCTTCACT GAGCATAACT CCAGTCCAAA TGTCAGTGGA 
AGCCTCTCCT CTGGGCTGCA GAAAATATTT GAAGACCCCA CTGACAGTGA TTTGCATAAA 
CTAAAATCTC CAAGCCAGGA CAACACAGAT GGCTATTTCA GAGGGAAAAC GTTATTGCTG 
GTTCAGCAGG CCTCCTCCCA GAGCATGACC TATTCTGAAA AGGATGAAAA GGAAAGTAGC 
CTTCCTAACG GTCGGAGCAT CTCCCTCATG GACCTTCAAG ACACTCACGC TGCTCAAGTA 
GAGCATGCAT CTATCATGCT CGATGTGCCT ATGCGCTTAA CTGGAAGCCA GCTTTCCATA 
ACCCAGGTGG CCAGTATCAA ACAACTGCGG GAAACCCAGA GCACTCCCCA GAGTGCACCC 
CAAGTGCGAA GGCCCTTGCA CCCAGCCTTG AACCAGCCAG GCAGCCTCCA GCCCCTGTCA 
TTCCAGAACC CTGTCTATCA CCTCAATAAC CCAATCCCAG CAATGCCAAA GGCCTCTGTG 
GATTCTAGTT TGGAGAATCT AAGCACTGCC AGTTCCAGAA GCCAAAGTAA CAGTGAAGAT 
TTCAAACTCA GTGGGCCCAG CAATAGCAGC ATGGAAGATT TCACTAAACG TAGCACGCAG 
AGCGAGGATT TCTCCAGGCG GCACACTGTA CCAGACAGAC ACATACCTCT TGCTCTGCCG 
CGGCAGAATA GTACCGGGCA GGCCCAGATC CGGAAAATGG ACCAGGCTGG GTTAGGTGCC 
CGAGCCAAAG CCCCGCCGTC CTTGCCACAC AGTGCTTCCT TACGTAGCAC GGGGAGCATG 
TCGGTGGCCT CTGCGGCCCT GGTGGCTGAA CCTGTGCAGA ATGGGAGCCG GTCCCGGCAG 
CAGTCCTCTT CCTCCAGAGA GAGCCCCGTT CCCAAAGTGA GAGCGATCCA GAGACAACAG 
ACACAGCAGG TGCAGTCACC TGTGGACTCT GCCACCATGT CCCCAGTAGA GAGGACAGCA 
GCCTGGGTGT TGAACAATGG ACAGTATGAA GAGGATGTGG AAGAGACTGA GCAAAATCCA 
GATGAATCCA AGCATGCTGA GAAGTATGAG CAGGAAATTA CTAAACTGAA GGAGCGCCTG 
AGAGTGTCCA GCCGGCGCCT TGAGGAATAT GAGCGCCGCT TGCTGGTGCA GGAGCAGCAG 
ATGCAGAAGC TGCTGCTGGA ATACAAGGCC AGGCTGGAGG ACAGCGAGGA GCGCCTGCGC 
AGGCAGCAGG AGGAGAAGGA CAGCCAGATG AAAAGCATCA TCAGCAGGCT GATGGCTGTG 
GAAGAGGAAC TGAAGAAGGA TCATGCTGAG ATGCAAGCAG TTATTGATGC AAAGCAAAAA 
ATAATCGATG CACAGGTCAT AAATGGAGAT GAGATTATTC AAACAGGAAA AACGAATCGT 
GTCCCTGGAT TCGGCCAACA CCAGACTGAT GAGCGCACTG ACCCAAGTGA AGGAGCGGTA 
CAGCATGCAG GTCCGCAATG GCATCTCCCC CACCAACCCC ACCAAGCTTT CCATCACGGA 
GAATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_014115154.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013970629.1 Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe37436
Clone ID Related Accession (Same CDS sequence)	XM_014115154.1 , XM_014115154.2
Accession Version	XM_014115154.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4269bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	ras GTPase-activating protein nGAP isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTCGT GGAAGATGAG CATCTTTAAG AGACAGACAT CACCTTGTCC TCCAAGCTAT 
CGAAGATCTT TGTCAGAACC TGCTTATGCA AAATCAGAGA TAGGAATAAG CTTAAAGAGT 
TATCCATGGC GTGGTCCAGT GAAAATCAAG AGGAAGAAAA ATGAACATGC TGTTTCCAGT 
CTAGGAAAGC CACTGGGTGC TTATCGGTGG CAAACACATC CCAGCTGTAG GTGGAAAGGG 
GGCAAAAGGG AGAGGTTACA GACTAATAAA AGAGACTTGG ATGAACGGCA ACAAAAGGTC 
CTGCCAGAGA TGGTTGGTAG TAATGCTTCT TCCTCTGCTA TTGAGGTCCT TGGAGCACCA 
ACAGAGAAGC TGAACCTGGT CAACACCCGG TCACTTCGGG TTCCATTCAC CAGATCTATT 
TCAGAGCCAG AACCTCGCTG CAGTATCAGA TTTGTTAGAT CATGGCCGAC TACAGGAAGG 
TCAGAGGAGT CTGAACAGCA GGGATATTTC ATCCGTGGCA TTTTCTCTAC TCTTTCCAAT 
GGCTTCTCCA GGATGCTGAG TCTGAAAGGA GAATCAACCA ATGAGCCAGT AAGAGAAGAT 
GTGAAGGGGC CACCTACCCA TCGTCTGTCT TGTGGACAGT CGCCCTACAC AGAGACGACA 
ACATGGGAGC GGAAGTATTG CATCCTCACC GACAGCCAGT TGGTGTTGCT CAACAAGGAG 
AAGGAGATAC CTGTGGAGGG AGGACAGGAG CAGCAGACAG ATTCCACCAA AGGGCGATGC 
CTGAGGAGAA CCGTCAGTGT CCCTTCCGAG GGCCAGTTTC CTGAGTACCC ACCAGAGGGC 
GCCACCAAAC TGGAGGTGCC GGCAGAAAGG TCCCCACGCA GACGGAGTAT CTCAGGGACC 
AGTACATCAG AGAAACCCAA CTCCATGGAC ACTGTAAATA CCTCACCCTT CAAAGTACCA 
GGGTTCTTCA GCAAGCGCCT GAAAGGCTCC ATCAAGAGGA CCAAAAGCCA ATCAAAGCTT 
GACAGAAACA CGAGCTTTCG GCTACCATCC CTTCGTAATG CAGATGACAG GACTCGCGGT 
CTGCCTAAAC TAAAAGAGTC ACGTTCCCAT GAGTCATTGC TGAGCCCTTG TAGTGCAGTG 
GAATGTCTGG ATCTTGGCAG AGGAGAGCCT GTGTCAGTGA AGCCACTCCA TAGCAGCATC 
CTTGGACAAG ACTTCTGCTT TGAGGTTACG TACTTAAGTG GAAGTAAATG CTTCAGCTGT 
AACTCTGCTT CAGAGAGAGA TAAGTGGATG GAGAACCTTC GTAGGACAGT TCAACCAAAT 
AAGGACAATT GCAGACGAGC TGAAAACGTT CTTCGTTTAT GGATCATTGA AGCCAAGGAC 
CTTGCCCCCA AAAAGAAATA TTTCTGTGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT 
ACAACCAGCA AGACCAAAGC AGATAATATT TTCTGGGGTG AACATTTTGA ATTTTACAGC 
CTTCCACCTC TTCATAGCAT CACAGTTCAC ATTTATAAAG ATGTGGAAAA AAAGAAAAAA 
AAAGACAAGA ATAATTATGT AGGGCTAGTC AACATTCCCA CTGCCAGTGT GACTGGGCGC 
CAATTTGTAG AAAAGTGGTA TCCAGTGAGT ACCCCTACAC CCAACAAAGG AAAGACCGGA 
GGACCTTCTA TTCGGATTAA ATCACGTTTC CAAACTATCA CCATTCTGCC TATGGAGCAA 
TACAAAGAAT TTGCAGAATT TGTCACCAGC AACTATACCA TGCTGTGTTC TGTCCTTGAA 
CCAGTAATTA GTGTGAGAAA TAAAGAGGAG TTGGCCTGTG CCCTAGTGCA CATTCTTCAA 
AGTACTGGCA GAGCCAAGGA TTTTCTGACC GACTTGGTGA TGTCTGAGGT GGATCGCTGT 
GGAGAGCATG ACGTCTTGAT CTTCAGAGAG AACACCATTG CTACCAAATC CATTGAGGAA 
TACCTCAAGT TGGTGGGACA ACAGTATCTT CATGACGCAT TGGGGGAGTT CATCAAAGCT 
TTGTACGAGT CAGATGAGAA CTGTGAAGTG GATCCCAGCA AGTGTTCCTC TAGTGAACTG 
ATAGACCATC AGAGCAACCT GAAGATGTGC TGTGAGCTGG CTTTCTGCAA GATCATCAAC 
TCTTACTGTG TTTTCCCTCG TGAGTTGAAA GAAGTGTTTG CATCGTGGAA GCAGCAGTGC 
CTGAACCGTG GCAAACAGGA CATCAGCGAG CGGCTCATCA GTGCCTCATT GTTTCTCCGT 
TTCCTGTGCC CTGCCATCAT GTCTCCCAGT CTCTTCAACC TTATGCAAGA GTACCCTGAT 
GACCGTACAT CTCGCACCCT GACTCTGATT GCCAAGGTCA TTCAGAACCT GGCTAACTTT 
GCCAAGTTTG GTAACAAAGA AGAATATATG GCATTCATGA ATGATTTTTT AGAACATGAA 
TGGGGTGGAA TGAAGCGCTT TCTTTTGGAA ATCTCTAATC CGGACACCAT CTCAAACACC 
CCAGGCTTTG ATGGTTACAT TGATCTGGGC CGAGAGCTTT CAGTCTTGCA TTCCTTACTA 
TGGGAAGTAG TTTCCCAGCT TGATAAGGGT GAAAATTCCT TCCTACAGGC GACCGTGGCA 
AAATTGGGAC CGCTCCCTCG AGTTCTTGCT GATATTACCA AGTCTCTAAC TAATCCTACA 
CCAATACAAC AGCAGCTGAG ACGCTTCACT GAGCATAACT CCAGTCCAAA TGTCAGTGGA 
AGCCTCTCCT CTGGGCTGCA GAAAATATTT GAAGACCCCA CTGACAGTGA TTTGCATAAA 
CTAAAATCTC CAAGCCAGGA CAACACAGAT GGCTATTTCA GAGGGAAAAC GTTATTGCTG 
GTTCAGCAGG CCTCCTCCCA GAGCATGACC TATTCTGAAA AGGATGAAAA GGAAAGTAGC 
CTTCCTAACG GTCGGAGCAT CTCCCTCATG GACCTTCAAG ACACTCACGC TGCTCAAGTA 
GAGCATGCAT CTATCATGCT CGATGTGCCT ATGCGCTTAA CTGGAAGCCA GCTTTCCATA 
ACCCAGGTGG CCAGTATCAA ACAACTGCGG GAAACCCAGA GCACTCCCCA GAGTGCACCC 
CAAGTGCGAA GGCCCTTGCA CCCAGCCTTG AACCAGCCAG GCAGCCTCCA GCCCCTGTCA 
TTCCAGAACC CTGTCTATCA CCTCAATAAC CCAATCCCAG CAATGCCAAA GGCCTCTGTG 
GATTCTAGTT TGGAGAATCT AAGCACTGCC AGTTCCAGAA GCCAAAGTAA CAGTGAAGAT 
TTCAAACTCA GTGGGCCCAG CAATAGCAGC ATGGAAGATT TCACTAAACG TAGCACGCAG 
AGCGAGGATT TCTCCAGGCG GCACACTGTA CCAGACAGAC ACATACCTCT TGCTCTGCCG 
CGGCAGAATA GTACCGGGCA GGCCCAGATC CGGAAAATGG ACCAGGCTGG GTTAGGTGCC 
CGAGCCAAAG CCCCGCCGTC CTTGCCACAC AGTGCTTCCT TACGTAGCAC GGGGAGCATG 
TCGGTGGCCT CTGCGGCCCT GGTGGCTGAA CCTGTGCAGA ATGGGAGCCG GTCCCGGCAG 
CAGTCCTCTT CCTCCAGAGA GAGCCCCGTT CCCAAAGTGA GAGCGATCCA GAGACAACAG 
ACACAGCAGG TGCAGTCACC TGTGGACTCT GCCACCATGT CCCCAGTAGA GAGGACAGCA 
GCCTGGGTGT TGAACAATGG ACAGTATGAA GAGGATGTGG AAGAGACTGA GCAAAATCCA 
GATGAATCCA AGCATGCTGA GAAGTATGAG CAGGAAATTA CTAAACTGAA GGAGCGCCTG 
AGAGTGTCCA GCCGGCGCCT TGAGGAATAT GAGCGCCGCT TGCTGGTGCA GGAGCAGCAG 
ATGCAGAAGC TGCTGCTGGA ATACAAGGCC AGGCTGGAGG ACAGCGAGGA GCGCCTGCGC 
AGGCAGCAGG AGGAGAAGGA CAGCCAGATG AAAAGCATCA TCAGCAGGCT GATGGCTGTG 
GAAGAGGAAC TGAAGAAGGA TCATGCTGAG ATGCAAGCAG TTATTGATGC AAAGCAAAAA 
ATAATCGATG CACAGGTCAT AAATGGAGAT GAGATTATTC AAACAGGAAA AACGAATCGT 
GTCCCTGGAT TCGGCCAACA CCAGACTGAT GAGCGCACTG ACCCAAGTGA AGGAGCGGTA 
CAGCATGCAG GTCCGCAATG GCATCTCCCC CACCAACCCC ACCAAGCTTT CCATCACGGA 
GAATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013970629.1
CDS	303..4571
Translation	MESWKMSIFKRQTSPCPPSYRRSLSEPAYAKSEIGISLKSYPWRGPVKIKRKKNEHAVSSLGKPLGAYRWQTHPSCRWKGGKRERLQTNKRDLDERQQKVLPEMVGSNASSSAIEVLGAPTEKLNLVNTRSLRVPFTRSISEPEPRCSIRFVRSWPTTGRSEESEQQGYFIRGIFSTLSNGFSRMLSLKGESTNEPVREDVKGPPTHRLSCGQSPYTETTTWERKYCILTDSQLVLLNKEKEIPVEGGQEQQTDSTKGRCLRRTVSVPSEGQFPEYPPEGATKLEVPAERSPRRRSISGTSTSEKPNSMDTVNTSPFKVPGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNADDRTRGLPKLKESRSHESLLSPCSAVECLDLGRGEPVSVKPLHSSILGQDFCFEVTYLSGSKCFSCNSASERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYSLPPLHSITVHIYKDVEKKKKKDKNNYVGLVNIPTASVTGRQFVEKWYPVSTPTPNKGKTGGPSIRIKSRFQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEELACALVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTIATKSIEEYLKLVGQQYLHDALGEFIKALYESDENCEVDPSKCSSSELIDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCLNRGKQDISERLISASLFLRFLCPAIMSPSLFNLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNTPGFDGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVAKLGPLPRVLADITKSLTNPTPIQQQLRRFTEHNSSPNVSGSLSSGLQKIFEDPTDSDLHKLKSPSQDNTDGYFRGKTLLLVQQASSQSMTYSEKDEKESSLPNGRSISLMDLQDTHAAQVEHASIMLDVPMRLTGSQLSITQVASIKQLRETQSTPQSAPQVRRPLHPALNQPGSLQPLSFQNPVYHLNNPIPAMPKASVDSSLENLSTASSRSQSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTVPDRHIPLALPRQNSTGQAQIRKMDQAGLGARAKAPPSLPHSASLRSTGSMSVASAALVAEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEDVEETEQNPDESKHAEKYEQEITKLKERLRVSSRRLEEYERRLLVQEQQMQKLLLEYKARLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQVINGDEIIQTGKTNRVPGFGQHQTDERTDPSEGAVQHAGPQWHLPHQPHQAFHHGEW

Target ORF information:

RefSeq Version	XM_014115154.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014115154.1

ORF Insert Sequence:

ATGGAGTCGT GGAAGATGAG CATCTTTAAG AGACAGACAT CACCTTGTCC TCCAAGCTAT 
CGAAGATCTT TGTCAGAACC TGCTTATGCA AAATCAGAGA TAGGAATAAG CTTAAAGAGT 
TATCCATGGC GTGGTCCAGT GAAAATCAAG AGGAAGAAAA ATGAACATGC TGTTTCCAGT 
CTAGGAAAGC CACTGGGTGC TTATCGGTGG CAAACACATC CCAGCTGTAG GTGGAAAGGG 
GGCAAAAGGG AGAGGTTACA GACTAATAAA AGAGACTTGG ATGAACGGCA ACAAAAGGTC 
CTGCCAGAGA TGGTTGGTAG TAATGCTTCT TCCTCTGCTA TTGAGGTCCT TGGAGCACCA 
ACAGAGAAGC TGAACCTGGT CAACACCCGG TCACTTCGGG TTCCATTCAC CAGATCTATT 
TCAGAGCCAG AACCTCGCTG CAGTATCAGA TTTGTTAGAT CATGGCCGAC TACAGGAAGG 
TCAGAGGAGT CTGAACAGCA GGGATATTTC ATCCGTGGCA TTTTCTCTAC TCTTTCCAAT 
GGCTTCTCCA GGATGCTGAG TCTGAAAGGA GAATCAACCA ATGAGCCAGT AAGAGAAGAT 
GTGAAGGGGC CACCTACCCA TCGTCTGTCT TGTGGACAGT CGCCCTACAC AGAGACGACA 
ACATGGGAGC GGAAGTATTG CATCCTCACC GACAGCCAGT TGGTGTTGCT CAACAAGGAG 
AAGGAGATAC CTGTGGAGGG AGGACAGGAG CAGCAGACAG ATTCCACCAA AGGGCGATGC 
CTGAGGAGAA CCGTCAGTGT CCCTTCCGAG GGCCAGTTTC CTGAGTACCC ACCAGAGGGC 
GCCACCAAAC TGGAGGTGCC GGCAGAAAGG TCCCCACGCA GACGGAGTAT CTCAGGGACC 
AGTACATCAG AGAAACCCAA CTCCATGGAC ACTGTAAATA CCTCACCCTT CAAAGTACCA 
GGGTTCTTCA GCAAGCGCCT GAAAGGCTCC ATCAAGAGGA CCAAAAGCCA ATCAAAGCTT 
GACAGAAACA CGAGCTTTCG GCTACCATCC CTTCGTAATG CAGATGACAG GACTCGCGGT 
CTGCCTAAAC TAAAAGAGTC ACGTTCCCAT GAGTCATTGC TGAGCCCTTG TAGTGCAGTG 
GAATGTCTGG ATCTTGGCAG AGGAGAGCCT GTGTCAGTGA AGCCACTCCA TAGCAGCATC 
CTTGGACAAG ACTTCTGCTT TGAGGTTACG TACTTAAGTG GAAGTAAATG CTTCAGCTGT 
AACTCTGCTT CAGAGAGAGA TAAGTGGATG GAGAACCTTC GTAGGACAGT TCAACCAAAT 
AAGGACAATT GCAGACGAGC TGAAAACGTT CTTCGTTTAT GGATCATTGA AGCCAAGGAC 
CTTGCCCCCA AAAAGAAATA TTTCTGTGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT 
ACAACCAGCA AGACCAAAGC AGATAATATT TTCTGGGGTG AACATTTTGA ATTTTACAGC 
CTTCCACCTC TTCATAGCAT CACAGTTCAC ATTTATAAAG ATGTGGAAAA AAAGAAAAAA 
AAAGACAAGA ATAATTATGT AGGGCTAGTC AACATTCCCA CTGCCAGTGT GACTGGGCGC 
CAATTTGTAG AAAAGTGGTA TCCAGTGAGT ACCCCTACAC CCAACAAAGG AAAGACCGGA 
GGACCTTCTA TTCGGATTAA ATCACGTTTC CAAACTATCA CCATTCTGCC TATGGAGCAA 
TACAAAGAAT TTGCAGAATT TGTCACCAGC AACTATACCA TGCTGTGTTC TGTCCTTGAA 
CCAGTAATTA GTGTGAGAAA TAAAGAGGAG TTGGCCTGTG CCCTAGTGCA CATTCTTCAA 
AGTACTGGCA GAGCCAAGGA TTTTCTGACC GACTTGGTGA TGTCTGAGGT GGATCGCTGT 
GGAGAGCATG ACGTCTTGAT CTTCAGAGAG AACACCATTG CTACCAAATC CATTGAGGAA 
TACCTCAAGT TGGTGGGACA ACAGTATCTT CATGACGCAT TGGGGGAGTT CATCAAAGCT 
TTGTACGAGT CAGATGAGAA CTGTGAAGTG GATCCCAGCA AGTGTTCCTC TAGTGAACTG 
ATAGACCATC AGAGCAACCT GAAGATGTGC TGTGAGCTGG CTTTCTGCAA GATCATCAAC 
TCTTACTGTG TTTTCCCTCG TGAGTTGAAA GAAGTGTTTG CATCGTGGAA GCAGCAGTGC 
CTGAACCGTG GCAAACAGGA CATCAGCGAG CGGCTCATCA GTGCCTCATT GTTTCTCCGT 
TTCCTGTGCC CTGCCATCAT GTCTCCCAGT CTCTTCAACC TTATGCAAGA GTACCCTGAT 
GACCGTACAT CTCGCACCCT GACTCTGATT GCCAAGGTCA TTCAGAACCT GGCTAACTTT 
GCCAAGTTTG GTAACAAAGA AGAATATATG GCATTCATGA ATGATTTTTT AGAACATGAA 
TGGGGTGGAA TGAAGCGCTT TCTTTTGGAA ATCTCTAATC CGGACACCAT CTCAAACACC 
CCAGGCTTTG ATGGTTACAT TGATCTGGGC CGAGAGCTTT CAGTCTTGCA TTCCTTACTA 
TGGGAAGTAG TTTCCCAGCT TGATAAGGGT GAAAATTCCT TCCTACAGGC GACCGTGGCA 
AAATTGGGAC CGCTCCCTCG AGTTCTTGCT GATATTACCA AGTCTCTAAC TAATCCTACA 
CCAATACAAC AGCAGCTGAG ACGCTTCACT GAGCATAACT CCAGTCCAAA TGTCAGTGGA 
AGCCTCTCCT CTGGGCTGCA GAAAATATTT GAAGACCCCA CTGACAGTGA TTTGCATAAA 
CTAAAATCTC CAAGCCAGGA CAACACAGAT GGCTATTTCA GAGGGAAAAC GTTATTGCTG 
GTTCAGCAGG CCTCCTCCCA GAGCATGACC TATTCTGAAA AGGATGAAAA GGAAAGTAGC 
CTTCCTAACG GTCGGAGCAT CTCCCTCATG GACCTTCAAG ACACTCACGC TGCTCAAGTA 
GAGCATGCAT CTATCATGCT CGATGTGCCT ATGCGCTTAA CTGGAAGCCA GCTTTCCATA 
ACCCAGGTGG CCAGTATCAA ACAACTGCGG GAAACCCAGA GCACTCCCCA GAGTGCACCC 
CAAGTGCGAA GGCCCTTGCA CCCAGCCTTG AACCAGCCAG GCAGCCTCCA GCCCCTGTCA 
TTCCAGAACC CTGTCTATCA CCTCAATAAC CCAATCCCAG CAATGCCAAA GGCCTCTGTG 
GATTCTAGTT TGGAGAATCT AAGCACTGCC AGTTCCAGAA GCCAAAGTAA CAGTGAAGAT 
TTCAAACTCA GTGGGCCCAG CAATAGCAGC ATGGAAGATT TCACTAAACG TAGCACGCAG 
AGCGAGGATT TCTCCAGGCG GCACACTGTA CCAGACAGAC ACATACCTCT TGCTCTGCCG 
CGGCAGAATA GTACCGGGCA GGCCCAGATC CGGAAAATGG ACCAGGCTGG GTTAGGTGCC 
CGAGCCAAAG CCCCGCCGTC CTTGCCACAC AGTGCTTCCT TACGTAGCAC GGGGAGCATG 
TCGGTGGCCT CTGCGGCCCT GGTGGCTGAA CCTGTGCAGA ATGGGAGCCG GTCCCGGCAG 
CAGTCCTCTT CCTCCAGAGA GAGCCCCGTT CCCAAAGTGA GAGCGATCCA GAGACAACAG 
ACACAGCAGG TGCAGTCACC TGTGGACTCT GCCACCATGT CCCCAGTAGA GAGGACAGCA 
GCCTGGGTGT TGAACAATGG ACAGTATGAA GAGGATGTGG AAGAGACTGA GCAAAATCCA 
GATGAATCCA AGCATGCTGA GAAGTATGAG CAGGAAATTA CTAAACTGAA GGAGCGCCTG 
AGAGTGTCCA GCCGGCGCCT TGAGGAATAT GAGCGCCGCT TGCTGGTGCA GGAGCAGCAG 
ATGCAGAAGC TGCTGCTGGA ATACAAGGCC AGGCTGGAGG ACAGCGAGGA GCGCCTGCGC 
AGGCAGCAGG AGGAGAAGGA CAGCCAGATG AAAAGCATCA TCAGCAGGCT GATGGCTGTG 
GAAGAGGAAC TGAAGAAGGA TCATGCTGAG ATGCAAGCAG TTATTGATGC AAAGCAAAAA 
ATAATCGATG CACAGGTCAT AAATGGAGAT GAGATTATTC AAACAGGAAA AACGAATCGT 
GTCCCTGGAT TCGGCCAACA CCAGACTGAT GAGCGCACTG ACCCAAGTGA AGGAGCGGTA 
CAGCATGCAG GTCCGCAATG GCATCTCCCC CACCAACCCC ACCAAGCTTT CCATCACGGA 
GAATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_014115154.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013970629.1 Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X1, mRNA.

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CloneID	OCe37436
Clone ID Related Accession (Same CDS sequence)	XM_014115154.1 , XM_014115154.2
Accession Version	XM_014115154.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4269bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	ras GTPase-activating protein nGAP isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014115154.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTCGT GGAAGATGAG CATCTTTAAG AGACAGACAT CACCTTGTCC TCCAAGCTAT 
CGAAGATCTT TGTCAGAACC TGCTTATGCA AAATCAGAGA TAGGAATAAG CTTAAAGAGT 
TATCCATGGC GTGGTCCAGT GAAAATCAAG AGGAAGAAAA ATGAACATGC TGTTTCCAGT 
CTAGGAAAGC CACTGGGTGC TTATCGGTGG CAAACACATC CCAGCTGTAG GTGGAAAGGG 
GGCAAAAGGG AGAGGTTACA GACTAATAAA AGAGACTTGG ATGAACGGCA ACAAAAGGTC 
CTGCCAGAGA TGGTTGGTAG TAATGCTTCT TCCTCTGCTA TTGAGGTCCT TGGAGCACCA 
ACAGAGAAGC TGAACCTGGT CAACACCCGG TCACTTCGGG TTCCATTCAC CAGATCTATT 
TCAGAGCCAG AACCTCGCTG CAGTATCAGA TTTGTTAGAT CATGGCCGAC TACAGGAAGG 
TCAGAGGAGT CTGAACAGCA GGGATATTTC ATCCGTGGCA TTTTCTCTAC TCTTTCCAAT 
GGCTTCTCCA GGATGCTGAG TCTGAAAGGA GAATCAACCA ATGAGCCAGT AAGAGAAGAT 
GTGAAGGGGC CACCTACCCA TCGTCTGTCT TGTGGACAGT CGCCCTACAC AGAGACGACA 
ACATGGGAGC GGAAGTATTG CATCCTCACC GACAGCCAGT TGGTGTTGCT CAACAAGGAG 
AAGGAGATAC CTGTGGAGGG AGGACAGGAG CAGCAGACAG ATTCCACCAA AGGGCGATGC 
CTGAGGAGAA CCGTCAGTGT CCCTTCCGAG GGCCAGTTTC CTGAGTACCC ACCAGAGGGC 
GCCACCAAAC TGGAGGTGCC GGCAGAAAGG TCCCCACGCA GACGGAGTAT CTCAGGGACC 
AGTACATCAG AGAAACCCAA CTCCATGGAC ACTGTAAATA CCTCACCCTT CAAAGTACCA 
GGGTTCTTCA GCAAGCGCCT GAAAGGCTCC ATCAAGAGGA CCAAAAGCCA ATCAAAGCTT 
GACAGAAACA CGAGCTTTCG GCTACCATCC CTTCGTAATG CAGATGACAG GACTCGCGGT 
CTGCCTAAAC TAAAAGAGTC ACGTTCCCAT GAGTCATTGC TGAGCCCTTG TAGTGCAGTG 
GAATGTCTGG ATCTTGGCAG AGGAGAGCCT GTGTCAGTGA AGCCACTCCA TAGCAGCATC 
CTTGGACAAG ACTTCTGCTT TGAGGTTACG TACTTAAGTG GAAGTAAATG CTTCAGCTGT 
AACTCTGCTT CAGAGAGAGA TAAGTGGATG GAGAACCTTC GTAGGACAGT TCAACCAAAT 
AAGGACAATT GCAGACGAGC TGAAAACGTT CTTCGTTTAT GGATCATTGA AGCCAAGGAC 
CTTGCCCCCA AAAAGAAATA TTTCTGTGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT 
ACAACCAGCA AGACCAAAGC AGATAATATT TTCTGGGGTG AACATTTTGA ATTTTACAGC 
CTTCCACCTC TTCATAGCAT CACAGTTCAC ATTTATAAAG ATGTGGAAAA AAAGAAAAAA 
AAAGACAAGA ATAATTATGT AGGGCTAGTC AACATTCCCA CTGCCAGTGT GACTGGGCGC 
CAATTTGTAG AAAAGTGGTA TCCAGTGAGT ACCCCTACAC CCAACAAAGG AAAGACCGGA 
GGACCTTCTA TTCGGATTAA ATCACGTTTC CAAACTATCA CCATTCTGCC TATGGAGCAA 
TACAAAGAAT TTGCAGAATT TGTCACCAGC AACTATACCA TGCTGTGTTC TGTCCTTGAA 
CCAGTAATTA GTGTGAGAAA TAAAGAGGAG TTGGCCTGTG CCCTAGTGCA CATTCTTCAA 
AGTACTGGCA GAGCCAAGGA TTTTCTGACC GACTTGGTGA TGTCTGAGGT GGATCGCTGT 
GGAGAGCATG ACGTCTTGAT CTTCAGAGAG AACACCATTG CTACCAAATC CATTGAGGAA 
TACCTCAAGT TGGTGGGACA ACAGTATCTT CATGACGCAT TGGGGGAGTT CATCAAAGCT 
TTGTACGAGT CAGATGAGAA CTGTGAAGTG GATCCCAGCA AGTGTTCCTC TAGTGAACTG 
ATAGACCATC AGAGCAACCT GAAGATGTGC TGTGAGCTGG CTTTCTGCAA GATCATCAAC 
TCTTACTGTG TTTTCCCTCG TGAGTTGAAA GAAGTGTTTG CATCGTGGAA GCAGCAGTGC 
CTGAACCGTG GCAAACAGGA CATCAGCGAG CGGCTCATCA GTGCCTCATT GTTTCTCCGT 
TTCCTGTGCC CTGCCATCAT GTCTCCCAGT CTCTTCAACC TTATGCAAGA GTACCCTGAT 
GACCGTACAT CTCGCACCCT GACTCTGATT GCCAAGGTCA TTCAGAACCT GGCTAACTTT 
GCCAAGTTTG GTAACAAAGA AGAATATATG GCATTCATGA ATGATTTTTT AGAACATGAA 
TGGGGTGGAA TGAAGCGCTT TCTTTTGGAA ATCTCTAATC CGGACACCAT CTCAAACACC 
CCAGGCTTTG ATGGTTACAT TGATCTGGGC CGAGAGCTTT CAGTCTTGCA TTCCTTACTA 
TGGGAAGTAG TTTCCCAGCT TGATAAGGGT GAAAATTCCT TCCTACAGGC GACCGTGGCA 
AAATTGGGAC CGCTCCCTCG AGTTCTTGCT GATATTACCA AGTCTCTAAC TAATCCTACA 
CCAATACAAC AGCAGCTGAG ACGCTTCACT GAGCATAACT CCAGTCCAAA TGTCAGTGGA 
AGCCTCTCCT CTGGGCTGCA GAAAATATTT GAAGACCCCA CTGACAGTGA TTTGCATAAA 
CTAAAATCTC CAAGCCAGGA CAACACAGAT GGCTATTTCA GAGGGAAAAC GTTATTGCTG 
GTTCAGCAGG CCTCCTCCCA GAGCATGACC TATTCTGAAA AGGATGAAAA GGAAAGTAGC 
CTTCCTAACG GTCGGAGCAT CTCCCTCATG GACCTTCAAG ACACTCACGC TGCTCAAGTA 
GAGCATGCAT CTATCATGCT CGATGTGCCT ATGCGCTTAA CTGGAAGCCA GCTTTCCATA 
ACCCAGGTGG CCAGTATCAA ACAACTGCGG GAAACCCAGA GCACTCCCCA GAGTGCACCC 
CAAGTGCGAA GGCCCTTGCA CCCAGCCTTG AACCAGCCAG GCAGCCTCCA GCCCCTGTCA 
TTCCAGAACC CTGTCTATCA CCTCAATAAC CCAATCCCAG CAATGCCAAA GGCCTCTGTG 
GATTCTAGTT TGGAGAATCT AAGCACTGCC AGTTCCAGAA GCCAAAGTAA CAGTGAAGAT 
TTCAAACTCA GTGGGCCCAG CAATAGCAGC ATGGAAGATT TCACTAAACG TAGCACGCAG 
AGCGAGGATT TCTCCAGGCG GCACACTGTA CCAGACAGAC ACATACCTCT TGCTCTGCCG 
CGGCAGAATA GTACCGGGCA GGCCCAGATC CGGAAAATGG ACCAGGCTGG GTTAGGTGCC 
CGAGCCAAAG CCCCGCCGTC CTTGCCACAC AGTGCTTCCT TACGTAGCAC GGGGAGCATG 
TCGGTGGCCT CTGCGGCCCT GGTGGCTGAA CCTGTGCAGA ATGGGAGCCG GTCCCGGCAG 
CAGTCCTCTT CCTCCAGAGA GAGCCCCGTT CCCAAAGTGA GAGCGATCCA GAGACAACAG 
ACACAGCAGG TGCAGTCACC TGTGGACTCT GCCACCATGT CCCCAGTAGA GAGGACAGCA 
GCCTGGGTGT TGAACAATGG ACAGTATGAA GAGGATGTGG AAGAGACTGA GCAAAATCCA 
GATGAATCCA AGCATGCTGA GAAGTATGAG CAGGAAATTA CTAAACTGAA GGAGCGCCTG 
AGAGTGTCCA GCCGGCGCCT TGAGGAATAT GAGCGCCGCT TGCTGGTGCA GGAGCAGCAG 
ATGCAGAAGC TGCTGCTGGA ATACAAGGCC AGGCTGGAGG ACAGCGAGGA GCGCCTGCGC 
AGGCAGCAGG AGGAGAAGGA CAGCCAGATG AAAAGCATCA TCAGCAGGCT GATGGCTGTG 
GAAGAGGAAC TGAAGAAGGA TCATGCTGAG ATGCAAGCAG TTATTGATGC AAAGCAAAAA 
ATAATCGATG CACAGGTCAT AAATGGAGAT GAGATTATTC AAACAGGAAA AACGAATCGT 
GTCCCTGGAT TCGGCCAACA CCAGACTGAT GAGCGCACTG ACCCAAGTGA AGGAGCGGTA 
CAGCATGCAG GTCCGCAATG GCATCTCCCC CACCAACCCC ACCAAGCTTT CCATCACGGA 
GAATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013970629.1
CDS	310..4578
Translation	MESWKMSIFKRQTSPCPPSYRRSLSEPAYAKSEIGISLKSYPWRGPVKIKRKKNEHAVSSLGKPLGAYRWQTHPSCRWKGGKRERLQTNKRDLDERQQKVLPEMVGSNASSSAIEVLGAPTEKLNLVNTRSLRVPFTRSISEPEPRCSIRFVRSWPTTGRSEESEQQGYFIRGIFSTLSNGFSRMLSLKGESTNEPVREDVKGPPTHRLSCGQSPYTETTTWERKYCILTDSQLVLLNKEKEIPVEGGQEQQTDSTKGRCLRRTVSVPSEGQFPEYPPEGATKLEVPAERSPRRRSISGTSTSEKPNSMDTVNTSPFKVPGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNADDRTRGLPKLKESRSHESLLSPCSAVECLDLGRGEPVSVKPLHSSILGQDFCFEVTYLSGSKCFSCNSASERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYSLPPLHSITVHIYKDVEKKKKKDKNNYVGLVNIPTASVTGRQFVEKWYPVSTPTPNKGKTGGPSIRIKSRFQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEELACALVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTIATKSIEEYLKLVGQQYLHDALGEFIKALYESDENCEVDPSKCSSSELIDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCLNRGKQDISERLISASLFLRFLCPAIMSPSLFNLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNTPGFDGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVAKLGPLPRVLADITKSLTNPTPIQQQLRRFTEHNSSPNVSGSLSSGLQKIFEDPTDSDLHKLKSPSQDNTDGYFRGKTLLLVQQASSQSMTYSEKDEKESSLPNGRSISLMDLQDTHAAQVEHASIMLDVPMRLTGSQLSITQVASIKQLRETQSTPQSAPQVRRPLHPALNQPGSLQPLSFQNPVYHLNNPIPAMPKASVDSSLENLSTASSRSQSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTVPDRHIPLALPRQNSTGQAQIRKMDQAGLGARAKAPPSLPHSASLRSTGSMSVASAALVAEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEDVEETEQNPDESKHAEKYEQEITKLKERLRVSSRRLEEYERRLLVQEQQMQKLLLEYKARLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQVINGDEIIQTGKTNRVPGFGQHQTDERTDPSEGAVQHAGPQWHLPHQPHQAFHHGEW

Target ORF information:

RefSeq Version	XM_014115154.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014115154.2

ORF Insert Sequence:

ATGGAGTCGT GGAAGATGAG CATCTTTAAG AGACAGACAT CACCTTGTCC TCCAAGCTAT 
CGAAGATCTT TGTCAGAACC TGCTTATGCA AAATCAGAGA TAGGAATAAG CTTAAAGAGT 
TATCCATGGC GTGGTCCAGT GAAAATCAAG AGGAAGAAAA ATGAACATGC TGTTTCCAGT 
CTAGGAAAGC CACTGGGTGC TTATCGGTGG CAAACACATC CCAGCTGTAG GTGGAAAGGG 
GGCAAAAGGG AGAGGTTACA GACTAATAAA AGAGACTTGG ATGAACGGCA ACAAAAGGTC 
CTGCCAGAGA TGGTTGGTAG TAATGCTTCT TCCTCTGCTA TTGAGGTCCT TGGAGCACCA 
ACAGAGAAGC TGAACCTGGT CAACACCCGG TCACTTCGGG TTCCATTCAC CAGATCTATT 
TCAGAGCCAG AACCTCGCTG CAGTATCAGA TTTGTTAGAT CATGGCCGAC TACAGGAAGG 
TCAGAGGAGT CTGAACAGCA GGGATATTTC ATCCGTGGCA TTTTCTCTAC TCTTTCCAAT 
GGCTTCTCCA GGATGCTGAG TCTGAAAGGA GAATCAACCA ATGAGCCAGT AAGAGAAGAT 
GTGAAGGGGC CACCTACCCA TCGTCTGTCT TGTGGACAGT CGCCCTACAC AGAGACGACA 
ACATGGGAGC GGAAGTATTG CATCCTCACC GACAGCCAGT TGGTGTTGCT CAACAAGGAG 
AAGGAGATAC CTGTGGAGGG AGGACAGGAG CAGCAGACAG ATTCCACCAA AGGGCGATGC 
CTGAGGAGAA CCGTCAGTGT CCCTTCCGAG GGCCAGTTTC CTGAGTACCC ACCAGAGGGC 
GCCACCAAAC TGGAGGTGCC GGCAGAAAGG TCCCCACGCA GACGGAGTAT CTCAGGGACC 
AGTACATCAG AGAAACCCAA CTCCATGGAC ACTGTAAATA CCTCACCCTT CAAAGTACCA 
GGGTTCTTCA GCAAGCGCCT GAAAGGCTCC ATCAAGAGGA CCAAAAGCCA ATCAAAGCTT 
GACAGAAACA CGAGCTTTCG GCTACCATCC CTTCGTAATG CAGATGACAG GACTCGCGGT 
CTGCCTAAAC TAAAAGAGTC ACGTTCCCAT GAGTCATTGC TGAGCCCTTG TAGTGCAGTG 
GAATGTCTGG ATCTTGGCAG AGGAGAGCCT GTGTCAGTGA AGCCACTCCA TAGCAGCATC 
CTTGGACAAG ACTTCTGCTT TGAGGTTACG TACTTAAGTG GAAGTAAATG CTTCAGCTGT 
AACTCTGCTT CAGAGAGAGA TAAGTGGATG GAGAACCTTC GTAGGACAGT TCAACCAAAT 
AAGGACAATT GCAGACGAGC TGAAAACGTT CTTCGTTTAT GGATCATTGA AGCCAAGGAC 
CTTGCCCCCA AAAAGAAATA TTTCTGTGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT 
ACAACCAGCA AGACCAAAGC AGATAATATT TTCTGGGGTG AACATTTTGA ATTTTACAGC 
CTTCCACCTC TTCATAGCAT CACAGTTCAC ATTTATAAAG ATGTGGAAAA AAAGAAAAAA 
AAAGACAAGA ATAATTATGT AGGGCTAGTC AACATTCCCA CTGCCAGTGT GACTGGGCGC 
CAATTTGTAG AAAAGTGGTA TCCAGTGAGT ACCCCTACAC CCAACAAAGG AAAGACCGGA 
GGACCTTCTA TTCGGATTAA ATCACGTTTC CAAACTATCA CCATTCTGCC TATGGAGCAA 
TACAAAGAAT TTGCAGAATT TGTCACCAGC AACTATACCA TGCTGTGTTC TGTCCTTGAA 
CCAGTAATTA GTGTGAGAAA TAAAGAGGAG TTGGCCTGTG CCCTAGTGCA CATTCTTCAA 
AGTACTGGCA GAGCCAAGGA TTTTCTGACC GACTTGGTGA TGTCTGAGGT GGATCGCTGT 
GGAGAGCATG ACGTCTTGAT CTTCAGAGAG AACACCATTG CTACCAAATC CATTGAGGAA 
TACCTCAAGT TGGTGGGACA ACAGTATCTT CATGACGCAT TGGGGGAGTT CATCAAAGCT 
TTGTACGAGT CAGATGAGAA CTGTGAAGTG GATCCCAGCA AGTGTTCCTC TAGTGAACTG 
ATAGACCATC AGAGCAACCT GAAGATGTGC TGTGAGCTGG CTTTCTGCAA GATCATCAAC 
TCTTACTGTG TTTTCCCTCG TGAGTTGAAA GAAGTGTTTG CATCGTGGAA GCAGCAGTGC 
CTGAACCGTG GCAAACAGGA CATCAGCGAG CGGCTCATCA GTGCCTCATT GTTTCTCCGT 
TTCCTGTGCC CTGCCATCAT GTCTCCCAGT CTCTTCAACC TTATGCAAGA GTACCCTGAT 
GACCGTACAT CTCGCACCCT GACTCTGATT GCCAAGGTCA TTCAGAACCT GGCTAACTTT 
GCCAAGTTTG GTAACAAAGA AGAATATATG GCATTCATGA ATGATTTTTT AGAACATGAA 
TGGGGTGGAA TGAAGCGCTT TCTTTTGGAA ATCTCTAATC CGGACACCAT CTCAAACACC 
CCAGGCTTTG ATGGTTACAT TGATCTGGGC CGAGAGCTTT CAGTCTTGCA TTCCTTACTA 
TGGGAAGTAG TTTCCCAGCT TGATAAGGGT GAAAATTCCT TCCTACAGGC GACCGTGGCA 
AAATTGGGAC CGCTCCCTCG AGTTCTTGCT GATATTACCA AGTCTCTAAC TAATCCTACA 
CCAATACAAC AGCAGCTGAG ACGCTTCACT GAGCATAACT CCAGTCCAAA TGTCAGTGGA 
AGCCTCTCCT CTGGGCTGCA GAAAATATTT GAAGACCCCA CTGACAGTGA TTTGCATAAA 
CTAAAATCTC CAAGCCAGGA CAACACAGAT GGCTATTTCA GAGGGAAAAC GTTATTGCTG 
GTTCAGCAGG CCTCCTCCCA GAGCATGACC TATTCTGAAA AGGATGAAAA GGAAAGTAGC 
CTTCCTAACG GTCGGAGCAT CTCCCTCATG GACCTTCAAG ACACTCACGC TGCTCAAGTA 
GAGCATGCAT CTATCATGCT CGATGTGCCT ATGCGCTTAA CTGGAAGCCA GCTTTCCATA 
ACCCAGGTGG CCAGTATCAA ACAACTGCGG GAAACCCAGA GCACTCCCCA GAGTGCACCC 
CAAGTGCGAA GGCCCTTGCA CCCAGCCTTG AACCAGCCAG GCAGCCTCCA GCCCCTGTCA 
TTCCAGAACC CTGTCTATCA CCTCAATAAC CCAATCCCAG CAATGCCAAA GGCCTCTGTG 
GATTCTAGTT TGGAGAATCT AAGCACTGCC AGTTCCAGAA GCCAAAGTAA CAGTGAAGAT 
TTCAAACTCA GTGGGCCCAG CAATAGCAGC ATGGAAGATT TCACTAAACG TAGCACGCAG 
AGCGAGGATT TCTCCAGGCG GCACACTGTA CCAGACAGAC ACATACCTCT TGCTCTGCCG 
CGGCAGAATA GTACCGGGCA GGCCCAGATC CGGAAAATGG ACCAGGCTGG GTTAGGTGCC 
CGAGCCAAAG CCCCGCCGTC CTTGCCACAC AGTGCTTCCT TACGTAGCAC GGGGAGCATG 
TCGGTGGCCT CTGCGGCCCT GGTGGCTGAA CCTGTGCAGA ATGGGAGCCG GTCCCGGCAG 
CAGTCCTCTT CCTCCAGAGA GAGCCCCGTT CCCAAAGTGA GAGCGATCCA GAGACAACAG 
ACACAGCAGG TGCAGTCACC TGTGGACTCT GCCACCATGT CCCCAGTAGA GAGGACAGCA 
GCCTGGGTGT TGAACAATGG ACAGTATGAA GAGGATGTGG AAGAGACTGA GCAAAATCCA 
GATGAATCCA AGCATGCTGA GAAGTATGAG CAGGAAATTA CTAAACTGAA GGAGCGCCTG 
AGAGTGTCCA GCCGGCGCCT TGAGGAATAT GAGCGCCGCT TGCTGGTGCA GGAGCAGCAG 
ATGCAGAAGC TGCTGCTGGA ATACAAGGCC AGGCTGGAGG ACAGCGAGGA GCGCCTGCGC 
AGGCAGCAGG AGGAGAAGGA CAGCCAGATG AAAAGCATCA TCAGCAGGCT GATGGCTGTG 
GAAGAGGAAC TGAAGAAGGA TCATGCTGAG ATGCAAGCAG TTATTGATGC AAAGCAAAAA 
ATAATCGATG CACAGGTCAT AAATGGAGAT GAGATTATTC AAACAGGAAA AACGAATCGT 
GTCCCTGGAT TCGGCCAACA CCAGACTGAT GAGCGCACTG ACCCAAGTGA AGGAGCGGTA 
CAGCATGCAG GTCCGCAATG GCATCTCCCC CACCAACCCC ACCAAGCTTT CCATCACGGA 
GAATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_014115155.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013970630.1 Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X5, mRNA.

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CloneID	OCe37437
Clone ID Related Accession (Same CDS sequence)	XM_014115155.2 , XM_014115155.1
Accession Version	XM_014115155.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3873bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	ras GTPase-activating protein nGAP isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014115155.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTCT CCCCGTCGTC CGGAGGAGCC GCCGAGGCGC TGTCCTGGCC GGACATGTTC 
CCGGCGCTGG AGTCCGACTC GCCGATACCG CCGGAGGAGC TGGAAGCGGT TCTCCCCGTC 
GGCGGGGCCG TGGCCGGGGG CATGCTGGAT CGGATCCTTC TGGAGTCGGT GTGCCAGCAG 
CAGAGCTGGG TCCGGGTGTA CGATGTGAAG GGGCCACCTA CCCATCGTCT GTCTTGTGGA 
CAGTCGCCCT ACACAGAGAC GACAACATGG GAGCGGAAGT ATTGCATCCT CACCGACAGC 
CAGTTGGTGT TGCTCAACAA GGAGAAGGAG ATACCTGTGG AGGGAGGACA GGAGCAGCAG 
ACAGATTCCA CCAAAGGGCG ATGCCTGAGG AGAACCGTCA GTGTCCCTTC CGAGGGCCAG 
TTTCCTGAGT ACCCACCAGA GGGCGCCACC AAACTGGAGG TGCCGGCAGA AAGGTCCCCA 
CGCAGACGGA GTATCTCAGG GACCAGTACA TCAGAGAAAC CCAACTCCAT GGACACTGTA 
AATACCTCAC CCTTCAAAGT ACCAGGGTTC TTCAGCAAGC GCCTGAAAGG CTCCATCAAG 
AGGACCAAAA GCCAATCAAA GCTTGACAGA AACACGAGCT TTCGGCTACC ATCCCTTCGT 
AATGCAGATG ACAGGACTCG CGGTCTGCCT AAACTAAAAG AGTCACGTTC CCATGAGTCA 
TTGCTGAGCC CTTGTAGTGC AGTGGAATGT CTGGATCTTG GCAGAGGAGA GCCTGTGTCA 
GTGAAGCCAC TCCATAGCAG CATCCTTGGA CAAGACTTCT GCTTTGAGGT TACGTACTTA 
AGTGGAAGTA AATGCTTCAG CTGTAACTCT GCTTCAGAGA GAGATAAGTG GATGGAGAAC 
CTTCGTAGGA CAGTTCAACC AAATAAGGAC AATTGCAGAC GAGCTGAAAA CGTTCTTCGT 
TTATGGATCA TTGAAGCCAA GGACCTTGCC CCCAAAAAGA AATATTTCTG TGAACTGTGC 
CTTGATGATA CCCTCTTTGC TCGTACAACC AGCAAGACCA AAGCAGATAA TATTTTCTGG 
GGTGAACATT TTGAATTTTA CAGCCTTCCA CCTCTTCATA GCATCACAGT TCACATTTAT 
AAAGATGTGG AAAAAAAGAA AAAAAAAGAC AAGAATAATT ATGTAGGGCT AGTCAACATT 
CCCACTGCCA GTGTGACTGG GCGCCAATTT GTAGAAAAGT GGTATCCAGT GAGTACCCCT 
ACACCCAACA AAGGAAAGAC CGGAGGACCT TCTATTCGGA TTAAATCACG TTTCCAAACT 
ATCACCATTC TGCCTATGGA GCAATACAAA GAATTTGCAG AATTTGTCAC CAGCAACTAT 
ACCATGCTGT GTTCTGTCCT TGAACCAGTA ATTAGTGTGA GAAATAAAGA GGAGTTGGCC 
TGTGCCCTAG TGCACATTCT TCAAAGTACT GGCAGAGCCA AGGATTTTCT GACCGACTTG 
GTGATGTCTG AGGTGGATCG CTGTGGAGAG CATGACGTCT TGATCTTCAG AGAGAACACC 
ATTGCTACCA AATCCATTGA GGAATACCTC AAGTTGGTGG GACAACAGTA TCTTCATGAC 
GCATTGGGGG AGTTCATCAA AGCTTTGTAC GAGTCAGATG AGAACTGTGA AGTGGATCCC 
AGCAAGTGTT CCTCTAGTGA ACTGATAGAC CATCAGAGCA ACCTGAAGAT GTGCTGTGAG 
CTGGCTTTCT GCAAGATCAT CAACTCTTAC TGTGTTTTCC CTCGTGAGTT GAAAGAAGTG 
TTTGCATCGT GGAAGCAGCA GTGCCTGAAC CGTGGCAAAC AGGACATCAG CGAGCGGCTC 
ATCAGTGCCT CATTGTTTCT CCGTTTCCTG TGCCCTGCCA TCATGTCTCC CAGTCTCTTC 
AACCTTATGC AAGAGTACCC TGATGACCGT ACATCTCGCA CCCTGACTCT GATTGCCAAG 
GTCATTCAGA ACCTGGCTAA CTTTGCCAAG TTTGGTAACA AAGAAGAATA TATGGCATTC 
ATGAATGATT TTTTAGAACA TGAATGGGGT GGAATGAAGC GCTTTCTTTT GGAAATCTCT 
AATCCGGACA CCATCTCAAA CACCCCAGGC TTTGATGGTT ACATTGATCT GGGCCGAGAG 
CTTTCAGTCT TGCATTCCTT ACTATGGGAA GTAGTTTCCC AGCTTGATAA GGGTGAAAAT 
TCCTTCCTAC AGGCGACCGT GGCAAAATTG GGACCGCTCC CTCGAGTTCT TGCTGATATT 
ACCAAGTCTC TAACTAATCC TACACCAATA CAACAGCAGC TGAGACGCTT CACTGAGCAT 
AACTCCAGTC CAAATGTCAG TGGAAGCCTC TCCTCTGGGC TGCAGAAAAT ATTTGAAGAC 
CCCACTGACA GTGATTTGCA TAAACTAAAA TCTCCAAGCC AGGACAACAC AGATGGCTAT 
TTCAGAGGGA AAACGTTATT GCTGGTTCAG CAGGCCTCCT CCCAGAGCAT GACCTATTCT 
GAAAAGGATG AAAAGGAAAG TAGCCTTCCT AACGGTCGGA GCATCTCCCT CATGGACCTT 
CAAGACACTC ACGCTGCTCA AGTAGAGCAT GCATCTATCA TGCTCGATGT GCCTATGCGC 
TTAACTGGAA GCCAGCTTTC CATAACCCAG GTGGCCAGTA TCAAACAACT GCGGGAAACC 
CAGAGCACTC CCCAGAGTGC ACCCCAAGTG CGAAGGCCCT TGCACCCAGC CTTGAACCAG 
CCAGGCAGCC TCCAGCCCCT GTCATTCCAG AACCCTGTCT ATCACCTCAA TAACCCAATC 
CCAGCAATGC CAAAGGCCTC TGTGGATTCT AGTTTGGAGA ATCTAAGCAC TGCCAGTTCC 
AGAAGCCAAA GTAACAGTGA AGATTTCAAA CTCAGTGGGC CCAGCAATAG CAGCATGGAA 
GATTTCACTA AACGTAGCAC GCAGAGCGAG GATTTCTCCA GGCGGCACAC TGTACCAGAC 
AGACACATAC CTCTTGCTCT GCCGCGGCAG AATAGTACCG GGCAGGCCCA GATCCGGAAA 
ATGGACCAGG CTGGGTTAGG TGCCCGAGCC AAAGCCCCGC CGTCCTTGCC ACACAGTGCT 
TCCTTACGTA GCACGGGGAG CATGTCGGTG GCCTCTGCGG CCCTGGTGGC TGAACCTGTG 
CAGAATGGGA GCCGGTCCCG GCAGCAGTCC TCTTCCTCCA GAGAGAGCCC CGTTCCCAAA 
GTGAGAGCGA TCCAGAGACA ACAGACACAG CAGGTGCAGT CACCTGTGGA CTCTGCCACC 
ATGTCCCCAG TAGAGAGGAC AGCAGCCTGG GTGTTGAACA ATGGACAGTA TGAAGAGGAT 
GTGGAAGAGA CTGAGCAAAA TCCAGATGAA TCCAAGCATG CTGAGAAGTA TGAGCAGGAA 
ATTACTAAAC TGAAGGAGCG CCTGAGAGTG TCCAGCCGGC GCCTTGAGGA ATATGAGCGC 
CGCTTGCTGG TGCAGGAGCA GCAGATGCAG AAGCTGCTGC TGGAATACAA GGCCAGGCTG 
GAGGACAGCG AGGAGCGCCT GCGCAGGCAG CAGGAGGAGA AGGACAGCCA GATGAAAAGC 
ATCATCAGCA GGCTGATGGC TGTGGAAGAG GAACTGAAGA AGGATCATGC TGAGATGCAA 
GCAGTTATTG ATGCAAAGCA AAAAATAATC GATGCACAGG TCATAAATGG AGATGAGATT 
ATTCAAACAG GAAAAACGAA TCGTGTCCCT GGATTCGGCC AACACCAGAC TGATGAGCGC 
ACTGACCCAA GTGAAGGAGC GGTACAGCAT GCAGGTCCGC AATGGCATCT CCCCCACCAA 
CCCCACCAAG CTTTCCATCA CGGAGAATGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013970630.1
CDS	35..3907
Translation	MELSPSSGGAAEALSWPDMFPALESDSPIPPEELEAVLPVGGAVAGGMLDRILLESVCQQQSWVRVYDVKGPPTHRLSCGQSPYTETTTWERKYCILTDSQLVLLNKEKEIPVEGGQEQQTDSTKGRCLRRTVSVPSEGQFPEYPPEGATKLEVPAERSPRRRSISGTSTSEKPNSMDTVNTSPFKVPGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNADDRTRGLPKLKESRSHESLLSPCSAVECLDLGRGEPVSVKPLHSSILGQDFCFEVTYLSGSKCFSCNSASERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYSLPPLHSITVHIYKDVEKKKKKDKNNYVGLVNIPTASVTGRQFVEKWYPVSTPTPNKGKTGGPSIRIKSRFQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEELACALVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTIATKSIEEYLKLVGQQYLHDALGEFIKALYESDENCEVDPSKCSSSELIDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCLNRGKQDISERLISASLFLRFLCPAIMSPSLFNLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNTPGFDGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVAKLGPLPRVLADITKSLTNPTPIQQQLRRFTEHNSSPNVSGSLSSGLQKIFEDPTDSDLHKLKSPSQDNTDGYFRGKTLLLVQQASSQSMTYSEKDEKESSLPNGRSISLMDLQDTHAAQVEHASIMLDVPMRLTGSQLSITQVASIKQLRETQSTPQSAPQVRRPLHPALNQPGSLQPLSFQNPVYHLNNPIPAMPKASVDSSLENLSTASSRSQSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTVPDRHIPLALPRQNSTGQAQIRKMDQAGLGARAKAPPSLPHSASLRSTGSMSVASAALVAEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEDVEETEQNPDESKHAEKYEQEITKLKERLRVSSRRLEEYERRLLVQEQQMQKLLLEYKARLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQVINGDEIIQTGKTNRVPGFGQHQTDERTDPSEGAVQHAGPQWHLPHQPHQAFHHGEW

Target ORF information:

RefSeq Version	XM_014115155.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014115155.2

ORF Insert Sequence:

ATGGAGCTCT CCCCGTCGTC CGGAGGAGCC GCCGAGGCGC TGTCCTGGCC GGACATGTTC 
CCGGCGCTGG AGTCCGACTC GCCGATACCG CCGGAGGAGC TGGAAGCGGT TCTCCCCGTC 
GGCGGGGCCG TGGCCGGGGG CATGCTGGAT CGGATCCTTC TGGAGTCGGT GTGCCAGCAG 
CAGAGCTGGG TCCGGGTGTA CGATGTGAAG GGGCCACCTA CCCATCGTCT GTCTTGTGGA 
CAGTCGCCCT ACACAGAGAC GACAACATGG GAGCGGAAGT ATTGCATCCT CACCGACAGC 
CAGTTGGTGT TGCTCAACAA GGAGAAGGAG ATACCTGTGG AGGGAGGACA GGAGCAGCAG 
ACAGATTCCA CCAAAGGGCG ATGCCTGAGG AGAACCGTCA GTGTCCCTTC CGAGGGCCAG 
TTTCCTGAGT ACCCACCAGA GGGCGCCACC AAACTGGAGG TGCCGGCAGA AAGGTCCCCA 
CGCAGACGGA GTATCTCAGG GACCAGTACA TCAGAGAAAC CCAACTCCAT GGACACTGTA 
AATACCTCAC CCTTCAAAGT ACCAGGGTTC TTCAGCAAGC GCCTGAAAGG CTCCATCAAG 
AGGACCAAAA GCCAATCAAA GCTTGACAGA AACACGAGCT TTCGGCTACC ATCCCTTCGT 
AATGCAGATG ACAGGACTCG CGGTCTGCCT AAACTAAAAG AGTCACGTTC CCATGAGTCA 
TTGCTGAGCC CTTGTAGTGC AGTGGAATGT CTGGATCTTG GCAGAGGAGA GCCTGTGTCA 
GTGAAGCCAC TCCATAGCAG CATCCTTGGA CAAGACTTCT GCTTTGAGGT TACGTACTTA 
AGTGGAAGTA AATGCTTCAG CTGTAACTCT GCTTCAGAGA GAGATAAGTG GATGGAGAAC 
CTTCGTAGGA CAGTTCAACC AAATAAGGAC AATTGCAGAC GAGCTGAAAA CGTTCTTCGT 
TTATGGATCA TTGAAGCCAA GGACCTTGCC CCCAAAAAGA AATATTTCTG TGAACTGTGC 
CTTGATGATA CCCTCTTTGC TCGTACAACC AGCAAGACCA AAGCAGATAA TATTTTCTGG 
GGTGAACATT TTGAATTTTA CAGCCTTCCA CCTCTTCATA GCATCACAGT TCACATTTAT 
AAAGATGTGG AAAAAAAGAA AAAAAAAGAC AAGAATAATT ATGTAGGGCT AGTCAACATT 
CCCACTGCCA GTGTGACTGG GCGCCAATTT GTAGAAAAGT GGTATCCAGT GAGTACCCCT 
ACACCCAACA AAGGAAAGAC CGGAGGACCT TCTATTCGGA TTAAATCACG TTTCCAAACT 
ATCACCATTC TGCCTATGGA GCAATACAAA GAATTTGCAG AATTTGTCAC CAGCAACTAT 
ACCATGCTGT GTTCTGTCCT TGAACCAGTA ATTAGTGTGA GAAATAAAGA GGAGTTGGCC 
TGTGCCCTAG TGCACATTCT TCAAAGTACT GGCAGAGCCA AGGATTTTCT GACCGACTTG 
GTGATGTCTG AGGTGGATCG CTGTGGAGAG CATGACGTCT TGATCTTCAG AGAGAACACC 
ATTGCTACCA AATCCATTGA GGAATACCTC AAGTTGGTGG GACAACAGTA TCTTCATGAC 
GCATTGGGGG AGTTCATCAA AGCTTTGTAC GAGTCAGATG AGAACTGTGA AGTGGATCCC 
AGCAAGTGTT CCTCTAGTGA ACTGATAGAC CATCAGAGCA ACCTGAAGAT GTGCTGTGAG 
CTGGCTTTCT GCAAGATCAT CAACTCTTAC TGTGTTTTCC CTCGTGAGTT GAAAGAAGTG 
TTTGCATCGT GGAAGCAGCA GTGCCTGAAC CGTGGCAAAC AGGACATCAG CGAGCGGCTC 
ATCAGTGCCT CATTGTTTCT CCGTTTCCTG TGCCCTGCCA TCATGTCTCC CAGTCTCTTC 
AACCTTATGC AAGAGTACCC TGATGACCGT ACATCTCGCA CCCTGACTCT GATTGCCAAG 
GTCATTCAGA ACCTGGCTAA CTTTGCCAAG TTTGGTAACA AAGAAGAATA TATGGCATTC 
ATGAATGATT TTTTAGAACA TGAATGGGGT GGAATGAAGC GCTTTCTTTT GGAAATCTCT 
AATCCGGACA CCATCTCAAA CACCCCAGGC TTTGATGGTT ACATTGATCT GGGCCGAGAG 
CTTTCAGTCT TGCATTCCTT ACTATGGGAA GTAGTTTCCC AGCTTGATAA GGGTGAAAAT 
TCCTTCCTAC AGGCGACCGT GGCAAAATTG GGACCGCTCC CTCGAGTTCT TGCTGATATT 
ACCAAGTCTC TAACTAATCC TACACCAATA CAACAGCAGC TGAGACGCTT CACTGAGCAT 
AACTCCAGTC CAAATGTCAG TGGAAGCCTC TCCTCTGGGC TGCAGAAAAT ATTTGAAGAC 
CCCACTGACA GTGATTTGCA TAAACTAAAA TCTCCAAGCC AGGACAACAC AGATGGCTAT 
TTCAGAGGGA AAACGTTATT GCTGGTTCAG CAGGCCTCCT CCCAGAGCAT GACCTATTCT 
GAAAAGGATG AAAAGGAAAG TAGCCTTCCT AACGGTCGGA GCATCTCCCT CATGGACCTT 
CAAGACACTC ACGCTGCTCA AGTAGAGCAT GCATCTATCA TGCTCGATGT GCCTATGCGC 
TTAACTGGAA GCCAGCTTTC CATAACCCAG GTGGCCAGTA TCAAACAACT GCGGGAAACC 
CAGAGCACTC CCCAGAGTGC ACCCCAAGTG CGAAGGCCCT TGCACCCAGC CTTGAACCAG 
CCAGGCAGCC TCCAGCCCCT GTCATTCCAG AACCCTGTCT ATCACCTCAA TAACCCAATC 
CCAGCAATGC CAAAGGCCTC TGTGGATTCT AGTTTGGAGA ATCTAAGCAC TGCCAGTTCC 
AGAAGCCAAA GTAACAGTGA AGATTTCAAA CTCAGTGGGC CCAGCAATAG CAGCATGGAA 
GATTTCACTA AACGTAGCAC GCAGAGCGAG GATTTCTCCA GGCGGCACAC TGTACCAGAC 
AGACACATAC CTCTTGCTCT GCCGCGGCAG AATAGTACCG GGCAGGCCCA GATCCGGAAA 
ATGGACCAGG CTGGGTTAGG TGCCCGAGCC AAAGCCCCGC CGTCCTTGCC ACACAGTGCT 
TCCTTACGTA GCACGGGGAG CATGTCGGTG GCCTCTGCGG CCCTGGTGGC TGAACCTGTG 
CAGAATGGGA GCCGGTCCCG GCAGCAGTCC TCTTCCTCCA GAGAGAGCCC CGTTCCCAAA 
GTGAGAGCGA TCCAGAGACA ACAGACACAG CAGGTGCAGT CACCTGTGGA CTCTGCCACC 
ATGTCCCCAG TAGAGAGGAC AGCAGCCTGG GTGTTGAACA ATGGACAGTA TGAAGAGGAT 
GTGGAAGAGA CTGAGCAAAA TCCAGATGAA TCCAAGCATG CTGAGAAGTA TGAGCAGGAA 
ATTACTAAAC TGAAGGAGCG CCTGAGAGTG TCCAGCCGGC GCCTTGAGGA ATATGAGCGC 
CGCTTGCTGG TGCAGGAGCA GCAGATGCAG AAGCTGCTGC TGGAATACAA GGCCAGGCTG 
GAGGACAGCG AGGAGCGCCT GCGCAGGCAG CAGGAGGAGA AGGACAGCCA GATGAAAAGC 
ATCATCAGCA GGCTGATGGC TGTGGAAGAG GAACTGAAGA AGGATCATGC TGAGATGCAA 
GCAGTTATTG ATGCAAAGCA AAAAATAATC GATGCACAGG TCATAAATGG AGATGAGATT 
ATTCAAACAG GAAAAACGAA TCGTGTCCCT GGATTCGGCC AACACCAGAC TGATGAGCGC 
ACTGACCCAA GTGAAGGAGC GGTACAGCAT GCAGGTCCGC AATGGCATCT CCCCCACCAA 
CCCCACCAAG CTTTCCATCA CGGAGAATGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_014115155.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013970630.1 Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe37437
Clone ID Related Accession (Same CDS sequence)	XM_014115155.2 , XM_014115155.1
Accession Version	XM_014115155.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3873bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	ras GTPase-activating protein nGAP isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTCT CCCCGTCGTC CGGAGGAGCC GCCGAGGCGC TGTCCTGGCC GGACATGTTC 
CCGGCGCTGG AGTCCGACTC GCCGATACCG CCGGAGGAGC TGGAAGCGGT TCTCCCCGTC 
GGCGGGGCCG TGGCCGGGGG CATGCTGGAT CGGATCCTTC TGGAGTCGGT GTGCCAGCAG 
CAGAGCTGGG TCCGGGTGTA CGATGTGAAG GGGCCACCTA CCCATCGTCT GTCTTGTGGA 
CAGTCGCCCT ACACAGAGAC GACAACATGG GAGCGGAAGT ATTGCATCCT CACCGACAGC 
CAGTTGGTGT TGCTCAACAA GGAGAAGGAG ATACCTGTGG AGGGAGGACA GGAGCAGCAG 
ACAGATTCCA CCAAAGGGCG ATGCCTGAGG AGAACCGTCA GTGTCCCTTC CGAGGGCCAG 
TTTCCTGAGT ACCCACCAGA GGGCGCCACC AAACTGGAGG TGCCGGCAGA AAGGTCCCCA 
CGCAGACGGA GTATCTCAGG GACCAGTACA TCAGAGAAAC CCAACTCCAT GGACACTGTA 
AATACCTCAC CCTTCAAAGT ACCAGGGTTC TTCAGCAAGC GCCTGAAAGG CTCCATCAAG 
AGGACCAAAA GCCAATCAAA GCTTGACAGA AACACGAGCT TTCGGCTACC ATCCCTTCGT 
AATGCAGATG ACAGGACTCG CGGTCTGCCT AAACTAAAAG AGTCACGTTC CCATGAGTCA 
TTGCTGAGCC CTTGTAGTGC AGTGGAATGT CTGGATCTTG GCAGAGGAGA GCCTGTGTCA 
GTGAAGCCAC TCCATAGCAG CATCCTTGGA CAAGACTTCT GCTTTGAGGT TACGTACTTA 
AGTGGAAGTA AATGCTTCAG CTGTAACTCT GCTTCAGAGA GAGATAAGTG GATGGAGAAC 
CTTCGTAGGA CAGTTCAACC AAATAAGGAC AATTGCAGAC GAGCTGAAAA CGTTCTTCGT 
TTATGGATCA TTGAAGCCAA GGACCTTGCC CCCAAAAAGA AATATTTCTG TGAACTGTGC 
CTTGATGATA CCCTCTTTGC TCGTACAACC AGCAAGACCA AAGCAGATAA TATTTTCTGG 
GGTGAACATT TTGAATTTTA CAGCCTTCCA CCTCTTCATA GCATCACAGT TCACATTTAT 
AAAGATGTGG AAAAAAAGAA AAAAAAAGAC AAGAATAATT ATGTAGGGCT AGTCAACATT 
CCCACTGCCA GTGTGACTGG GCGCCAATTT GTAGAAAAGT GGTATCCAGT GAGTACCCCT 
ACACCCAACA AAGGAAAGAC CGGAGGACCT TCTATTCGGA TTAAATCACG TTTCCAAACT 
ATCACCATTC TGCCTATGGA GCAATACAAA GAATTTGCAG AATTTGTCAC CAGCAACTAT 
ACCATGCTGT GTTCTGTCCT TGAACCAGTA ATTAGTGTGA GAAATAAAGA GGAGTTGGCC 
TGTGCCCTAG TGCACATTCT TCAAAGTACT GGCAGAGCCA AGGATTTTCT GACCGACTTG 
GTGATGTCTG AGGTGGATCG CTGTGGAGAG CATGACGTCT TGATCTTCAG AGAGAACACC 
ATTGCTACCA AATCCATTGA GGAATACCTC AAGTTGGTGG GACAACAGTA TCTTCATGAC 
GCATTGGGGG AGTTCATCAA AGCTTTGTAC GAGTCAGATG AGAACTGTGA AGTGGATCCC 
AGCAAGTGTT CCTCTAGTGA ACTGATAGAC CATCAGAGCA ACCTGAAGAT GTGCTGTGAG 
CTGGCTTTCT GCAAGATCAT CAACTCTTAC TGTGTTTTCC CTCGTGAGTT GAAAGAAGTG 
TTTGCATCGT GGAAGCAGCA GTGCCTGAAC CGTGGCAAAC AGGACATCAG CGAGCGGCTC 
ATCAGTGCCT CATTGTTTCT CCGTTTCCTG TGCCCTGCCA TCATGTCTCC CAGTCTCTTC 
AACCTTATGC AAGAGTACCC TGATGACCGT ACATCTCGCA CCCTGACTCT GATTGCCAAG 
GTCATTCAGA ACCTGGCTAA CTTTGCCAAG TTTGGTAACA AAGAAGAATA TATGGCATTC 
ATGAATGATT TTTTAGAACA TGAATGGGGT GGAATGAAGC GCTTTCTTTT GGAAATCTCT 
AATCCGGACA CCATCTCAAA CACCCCAGGC TTTGATGGTT ACATTGATCT GGGCCGAGAG 
CTTTCAGTCT TGCATTCCTT ACTATGGGAA GTAGTTTCCC AGCTTGATAA GGGTGAAAAT 
TCCTTCCTAC AGGCGACCGT GGCAAAATTG GGACCGCTCC CTCGAGTTCT TGCTGATATT 
ACCAAGTCTC TAACTAATCC TACACCAATA CAACAGCAGC TGAGACGCTT CACTGAGCAT 
AACTCCAGTC CAAATGTCAG TGGAAGCCTC TCCTCTGGGC TGCAGAAAAT ATTTGAAGAC 
CCCACTGACA GTGATTTGCA TAAACTAAAA TCTCCAAGCC AGGACAACAC AGATGGCTAT 
TTCAGAGGGA AAACGTTATT GCTGGTTCAG CAGGCCTCCT CCCAGAGCAT GACCTATTCT 
GAAAAGGATG AAAAGGAAAG TAGCCTTCCT AACGGTCGGA GCATCTCCCT CATGGACCTT 
CAAGACACTC ACGCTGCTCA AGTAGAGCAT GCATCTATCA TGCTCGATGT GCCTATGCGC 
TTAACTGGAA GCCAGCTTTC CATAACCCAG GTGGCCAGTA TCAAACAACT GCGGGAAACC 
CAGAGCACTC CCCAGAGTGC ACCCCAAGTG CGAAGGCCCT TGCACCCAGC CTTGAACCAG 
CCAGGCAGCC TCCAGCCCCT GTCATTCCAG AACCCTGTCT ATCACCTCAA TAACCCAATC 
CCAGCAATGC CAAAGGCCTC TGTGGATTCT AGTTTGGAGA ATCTAAGCAC TGCCAGTTCC 
AGAAGCCAAA GTAACAGTGA AGATTTCAAA CTCAGTGGGC CCAGCAATAG CAGCATGGAA 
GATTTCACTA AACGTAGCAC GCAGAGCGAG GATTTCTCCA GGCGGCACAC TGTACCAGAC 
AGACACATAC CTCTTGCTCT GCCGCGGCAG AATAGTACCG GGCAGGCCCA GATCCGGAAA 
ATGGACCAGG CTGGGTTAGG TGCCCGAGCC AAAGCCCCGC CGTCCTTGCC ACACAGTGCT 
TCCTTACGTA GCACGGGGAG CATGTCGGTG GCCTCTGCGG CCCTGGTGGC TGAACCTGTG 
CAGAATGGGA GCCGGTCCCG GCAGCAGTCC TCTTCCTCCA GAGAGAGCCC CGTTCCCAAA 
GTGAGAGCGA TCCAGAGACA ACAGACACAG CAGGTGCAGT CACCTGTGGA CTCTGCCACC 
ATGTCCCCAG TAGAGAGGAC AGCAGCCTGG GTGTTGAACA ATGGACAGTA TGAAGAGGAT 
GTGGAAGAGA CTGAGCAAAA TCCAGATGAA TCCAAGCATG CTGAGAAGTA TGAGCAGGAA 
ATTACTAAAC TGAAGGAGCG CCTGAGAGTG TCCAGCCGGC GCCTTGAGGA ATATGAGCGC 
CGCTTGCTGG TGCAGGAGCA GCAGATGCAG AAGCTGCTGC TGGAATACAA GGCCAGGCTG 
GAGGACAGCG AGGAGCGCCT GCGCAGGCAG CAGGAGGAGA AGGACAGCCA GATGAAAAGC 
ATCATCAGCA GGCTGATGGC TGTGGAAGAG GAACTGAAGA AGGATCATGC TGAGATGCAA 
GCAGTTATTG ATGCAAAGCA AAAAATAATC GATGCACAGG TCATAAATGG AGATGAGATT 
ATTCAAACAG GAAAAACGAA TCGTGTCCCT GGATTCGGCC AACACCAGAC TGATGAGCGC 
ACTGACCCAA GTGAAGGAGC GGTACAGCAT GCAGGTCCGC AATGGCATCT CCCCCACCAA 
CCCCACCAAG CTTTCCATCA CGGAGAATGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013970630.1
CDS	54..3926
Translation	MELSPSSGGAAEALSWPDMFPALESDSPIPPEELEAVLPVGGAVAGGMLDRILLESVCQQQSWVRVYDVKGPPTHRLSCGQSPYTETTTWERKYCILTDSQLVLLNKEKEIPVEGGQEQQTDSTKGRCLRRTVSVPSEGQFPEYPPEGATKLEVPAERSPRRRSISGTSTSEKPNSMDTVNTSPFKVPGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNADDRTRGLPKLKESRSHESLLSPCSAVECLDLGRGEPVSVKPLHSSILGQDFCFEVTYLSGSKCFSCNSASERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYSLPPLHSITVHIYKDVEKKKKKDKNNYVGLVNIPTASVTGRQFVEKWYPVSTPTPNKGKTGGPSIRIKSRFQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEELACALVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTIATKSIEEYLKLVGQQYLHDALGEFIKALYESDENCEVDPSKCSSSELIDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCLNRGKQDISERLISASLFLRFLCPAIMSPSLFNLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNTPGFDGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVAKLGPLPRVLADITKSLTNPTPIQQQLRRFTEHNSSPNVSGSLSSGLQKIFEDPTDSDLHKLKSPSQDNTDGYFRGKTLLLVQQASSQSMTYSEKDEKESSLPNGRSISLMDLQDTHAAQVEHASIMLDVPMRLTGSQLSITQVASIKQLRETQSTPQSAPQVRRPLHPALNQPGSLQPLSFQNPVYHLNNPIPAMPKASVDSSLENLSTASSRSQSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTVPDRHIPLALPRQNSTGQAQIRKMDQAGLGARAKAPPSLPHSASLRSTGSMSVASAALVAEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEDVEETEQNPDESKHAEKYEQEITKLKERLRVSSRRLEEYERRLLVQEQQMQKLLLEYKARLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQVINGDEIIQTGKTNRVPGFGQHQTDERTDPSEGAVQHAGPQWHLPHQPHQAFHHGEW

Target ORF information:

RefSeq Version	XM_014115155.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014115155.1

ORF Insert Sequence:

ATGGAGCTCT CCCCGTCGTC CGGAGGAGCC GCCGAGGCGC TGTCCTGGCC GGACATGTTC 
CCGGCGCTGG AGTCCGACTC GCCGATACCG CCGGAGGAGC TGGAAGCGGT TCTCCCCGTC 
GGCGGGGCCG TGGCCGGGGG CATGCTGGAT CGGATCCTTC TGGAGTCGGT GTGCCAGCAG 
CAGAGCTGGG TCCGGGTGTA CGATGTGAAG GGGCCACCTA CCCATCGTCT GTCTTGTGGA 
CAGTCGCCCT ACACAGAGAC GACAACATGG GAGCGGAAGT ATTGCATCCT CACCGACAGC 
CAGTTGGTGT TGCTCAACAA GGAGAAGGAG ATACCTGTGG AGGGAGGACA GGAGCAGCAG 
ACAGATTCCA CCAAAGGGCG ATGCCTGAGG AGAACCGTCA GTGTCCCTTC CGAGGGCCAG 
TTTCCTGAGT ACCCACCAGA GGGCGCCACC AAACTGGAGG TGCCGGCAGA AAGGTCCCCA 
CGCAGACGGA GTATCTCAGG GACCAGTACA TCAGAGAAAC CCAACTCCAT GGACACTGTA 
AATACCTCAC CCTTCAAAGT ACCAGGGTTC TTCAGCAAGC GCCTGAAAGG CTCCATCAAG 
AGGACCAAAA GCCAATCAAA GCTTGACAGA AACACGAGCT TTCGGCTACC ATCCCTTCGT 
AATGCAGATG ACAGGACTCG CGGTCTGCCT AAACTAAAAG AGTCACGTTC CCATGAGTCA 
TTGCTGAGCC CTTGTAGTGC AGTGGAATGT CTGGATCTTG GCAGAGGAGA GCCTGTGTCA 
GTGAAGCCAC TCCATAGCAG CATCCTTGGA CAAGACTTCT GCTTTGAGGT TACGTACTTA 
AGTGGAAGTA AATGCTTCAG CTGTAACTCT GCTTCAGAGA GAGATAAGTG GATGGAGAAC 
CTTCGTAGGA CAGTTCAACC AAATAAGGAC AATTGCAGAC GAGCTGAAAA CGTTCTTCGT 
TTATGGATCA TTGAAGCCAA GGACCTTGCC CCCAAAAAGA AATATTTCTG TGAACTGTGC 
CTTGATGATA CCCTCTTTGC TCGTACAACC AGCAAGACCA AAGCAGATAA TATTTTCTGG 
GGTGAACATT TTGAATTTTA CAGCCTTCCA CCTCTTCATA GCATCACAGT TCACATTTAT 
AAAGATGTGG AAAAAAAGAA AAAAAAAGAC AAGAATAATT ATGTAGGGCT AGTCAACATT 
CCCACTGCCA GTGTGACTGG GCGCCAATTT GTAGAAAAGT GGTATCCAGT GAGTACCCCT 
ACACCCAACA AAGGAAAGAC CGGAGGACCT TCTATTCGGA TTAAATCACG TTTCCAAACT 
ATCACCATTC TGCCTATGGA GCAATACAAA GAATTTGCAG AATTTGTCAC CAGCAACTAT 
ACCATGCTGT GTTCTGTCCT TGAACCAGTA ATTAGTGTGA GAAATAAAGA GGAGTTGGCC 
TGTGCCCTAG TGCACATTCT TCAAAGTACT GGCAGAGCCA AGGATTTTCT GACCGACTTG 
GTGATGTCTG AGGTGGATCG CTGTGGAGAG CATGACGTCT TGATCTTCAG AGAGAACACC 
ATTGCTACCA AATCCATTGA GGAATACCTC AAGTTGGTGG GACAACAGTA TCTTCATGAC 
GCATTGGGGG AGTTCATCAA AGCTTTGTAC GAGTCAGATG AGAACTGTGA AGTGGATCCC 
AGCAAGTGTT CCTCTAGTGA ACTGATAGAC CATCAGAGCA ACCTGAAGAT GTGCTGTGAG 
CTGGCTTTCT GCAAGATCAT CAACTCTTAC TGTGTTTTCC CTCGTGAGTT GAAAGAAGTG 
TTTGCATCGT GGAAGCAGCA GTGCCTGAAC CGTGGCAAAC AGGACATCAG CGAGCGGCTC 
ATCAGTGCCT CATTGTTTCT CCGTTTCCTG TGCCCTGCCA TCATGTCTCC CAGTCTCTTC 
AACCTTATGC AAGAGTACCC TGATGACCGT ACATCTCGCA CCCTGACTCT GATTGCCAAG 
GTCATTCAGA ACCTGGCTAA CTTTGCCAAG TTTGGTAACA AAGAAGAATA TATGGCATTC 
ATGAATGATT TTTTAGAACA TGAATGGGGT GGAATGAAGC GCTTTCTTTT GGAAATCTCT 
AATCCGGACA CCATCTCAAA CACCCCAGGC TTTGATGGTT ACATTGATCT GGGCCGAGAG 
CTTTCAGTCT TGCATTCCTT ACTATGGGAA GTAGTTTCCC AGCTTGATAA GGGTGAAAAT 
TCCTTCCTAC AGGCGACCGT GGCAAAATTG GGACCGCTCC CTCGAGTTCT TGCTGATATT 
ACCAAGTCTC TAACTAATCC TACACCAATA CAACAGCAGC TGAGACGCTT CACTGAGCAT 
AACTCCAGTC CAAATGTCAG TGGAAGCCTC TCCTCTGGGC TGCAGAAAAT ATTTGAAGAC 
CCCACTGACA GTGATTTGCA TAAACTAAAA TCTCCAAGCC AGGACAACAC AGATGGCTAT 
TTCAGAGGGA AAACGTTATT GCTGGTTCAG CAGGCCTCCT CCCAGAGCAT GACCTATTCT 
GAAAAGGATG AAAAGGAAAG TAGCCTTCCT AACGGTCGGA GCATCTCCCT CATGGACCTT 
CAAGACACTC ACGCTGCTCA AGTAGAGCAT GCATCTATCA TGCTCGATGT GCCTATGCGC 
TTAACTGGAA GCCAGCTTTC CATAACCCAG GTGGCCAGTA TCAAACAACT GCGGGAAACC 
CAGAGCACTC CCCAGAGTGC ACCCCAAGTG CGAAGGCCCT TGCACCCAGC CTTGAACCAG 
CCAGGCAGCC TCCAGCCCCT GTCATTCCAG AACCCTGTCT ATCACCTCAA TAACCCAATC 
CCAGCAATGC CAAAGGCCTC TGTGGATTCT AGTTTGGAGA ATCTAAGCAC TGCCAGTTCC 
AGAAGCCAAA GTAACAGTGA AGATTTCAAA CTCAGTGGGC CCAGCAATAG CAGCATGGAA 
GATTTCACTA AACGTAGCAC GCAGAGCGAG GATTTCTCCA GGCGGCACAC TGTACCAGAC 
AGACACATAC CTCTTGCTCT GCCGCGGCAG AATAGTACCG GGCAGGCCCA GATCCGGAAA 
ATGGACCAGG CTGGGTTAGG TGCCCGAGCC AAAGCCCCGC CGTCCTTGCC ACACAGTGCT 
TCCTTACGTA GCACGGGGAG CATGTCGGTG GCCTCTGCGG CCCTGGTGGC TGAACCTGTG 
CAGAATGGGA GCCGGTCCCG GCAGCAGTCC TCTTCCTCCA GAGAGAGCCC CGTTCCCAAA 
GTGAGAGCGA TCCAGAGACA ACAGACACAG CAGGTGCAGT CACCTGTGGA CTCTGCCACC 
ATGTCCCCAG TAGAGAGGAC AGCAGCCTGG GTGTTGAACA ATGGACAGTA TGAAGAGGAT 
GTGGAAGAGA CTGAGCAAAA TCCAGATGAA TCCAAGCATG CTGAGAAGTA TGAGCAGGAA 
ATTACTAAAC TGAAGGAGCG CCTGAGAGTG TCCAGCCGGC GCCTTGAGGA ATATGAGCGC 
CGCTTGCTGG TGCAGGAGCA GCAGATGCAG AAGCTGCTGC TGGAATACAA GGCCAGGCTG 
GAGGACAGCG AGGAGCGCCT GCGCAGGCAG CAGGAGGAGA AGGACAGCCA GATGAAAAGC 
ATCATCAGCA GGCTGATGGC TGTGGAAGAG GAACTGAAGA AGGATCATGC TGAGATGCAA 
GCAGTTATTG ATGCAAAGCA AAAAATAATC GATGCACAGG TCATAAATGG AGATGAGATT 
ATTCAAACAG GAAAAACGAA TCGTGTCCCT GGATTCGGCC AACACCAGAC TGATGAGCGC 
ACTGACCCAA GTGAAGGAGC GGTACAGCAT GCAGGTCCGC AATGGCATCT CCCCCACCAA 
CCCCACCAAG CTTTCCATCA CGGAGAATGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_014115156.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013970631.1 Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OCe37438
Clone ID Related Accession (Same CDS sequence)	XM_014115156.1
Accession Version	XM_014115156.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	ras GTPase-activating protein nGAP isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTGGA CTTGTAATAT CCTTAATGTT GCTGTTGGCA GTCAGTTACA GATTCCATTA 
TTGTCGATCA ATGCCGTCGC CATGCGAGTA ATTGTGTTCT GTATGGAAAA GTTTTCTGGC 
TGGAGAATGA GTCTCCTTTC CCCCGGTGAT GTGGCCGAGT GGCTGGATAG TAGCACGGAA 
GAGGAGAAGA GCCTTCTGTC ATTTCTGGAG GTGCCGGCAG AAAGGTCCCC ACGCAGACGG 
AGTATCTCAG GGACCAGTAC ATCAGAGAAA CCCAACTCCA TGGACACTGT AAATACCTCA 
CCCTTCAAAG TACCAGGGTT CTTCAGCAAG CGCCTGAAAG GCTCCATCAA GAGGACCAAA 
AGCCAATCAA AGCTTGACAG AAACACGAGC TTTCGGCTAC CATCCCTTCG TAATGCAGAT 
GACAGGACTC GCGGTCTGCC TAAACTAAAA GAGTCACGTT CCCATGAGTC ATTGCTGAGC 
CCTTGTAGTG CAGTGGAATG TCTGGATCTT GGCAGAGGAG AGCCTGTGTC AGTGAAGCCA 
CTCCATAGCA GCATCCTTGG ACAAGACTTC TGCTTTGAGG TTACGTACTT AAGTGGAAGT 
AAATGCTTCA GCTGTAACTC TGCTTCAGAG AGAGATAAGT GGATGGAGAA CCTTCGTAGG 
ACAGTTCAAC CAAATAAGGA CAATTGCAGA CGAGCTGAAA ACGTTCTTCG TTTATGGATC 
ATTGAAGCCA AGGACCTTGC CCCCAAAAAG AAATATTTCT GTGAACTGTG CCTTGATGAT 
ACCCTCTTTG CTCGTACAAC CAGCAAGACC AAAGCAGATA ATATTTTCTG GGGTGAACAT 
TTTGAATTTT ACAGCCTTCC ACCTCTTCAT AGCATCACAG TTCACATTTA TAAAGATGTG 
GAAAAAAAGA AAAAAAAAGA CAAGAATAAT TATGTAGGGC TAGTCAACAT TCCCACTGCC 
AGTGTGACTG GGCGCCAATT TGTAGAAAAG TGGTATCCAG TGAGTACCCC TACACCCAAC 
AAAGGAAAGA CCGGAGGACC TTCTATTCGG ATTAAATCAC GTTTCCAAAC TATCACCATT 
CTGCCTATGG AGCAATACAA AGAATTTGCA GAATTTGTCA CCAGCAACTA TACCATGCTG 
TGTTCTGTCC TTGAACCAGT AATTAGTGTG AGAAATAAAG AGGAGTTGGC CTGTGCCCTA 
GTGCACATTC TTCAAAGTAC TGGCAGAGCC AAGGATTTTC TGACCGACTT GGTGATGTCT 
GAGGTGGATC GCTGTGGAGA GCATGACGTC TTGATCTTCA GAGAGAACAC CATTGCTACC 
AAATCCATTG AGGAATACCT CAAGTTGGTG GGACAACAGT ATCTTCATGA CGCATTGGGG 
GAGTTCATCA AAGCTTTGTA CGAGTCAGAT GAGAACTGTG AAGTGGATCC CAGCAAGTGT 
TCCTCTAGTG AACTGATAGA CCATCAGAGC AACCTGAAGA TGTGCTGTGA GCTGGCTTTC 
TGCAAGATCA TCAACTCTTA CTGTGTTTTC CCTCGTGAGT TGAAAGAAGT GTTTGCATCG 
TGGAAGCAGC AGTGCCTGAA CCGTGGCAAA CAGGACATCA GCGAGCGGCT CATCAGTGCC 
TCATTGTTTC TCCGTTTCCT GTGCCCTGCC ATCATGTCTC CCAGTCTCTT CAACCTTATG 
CAAGAGTACC CTGATGACCG TACATCTCGC ACCCTGACTC TGATTGCCAA GGTCATTCAG 
AACCTGGCTA ACTTTGCCAA GTTTGGTAAC AAAGAAGAAT ATATGGCATT CATGAATGAT 
TTTTTAGAAC ATGAATGGGG TGGAATGAAG CGCTTTCTTT TGGAAATCTC TAATCCGGAC 
ACCATCTCAA ACACCCCAGG CTTTGATGGT TACATTGATC TGGGCCGAGA GCTTTCAGTC 
TTGCATTCCT TACTATGGGA AGTAGTTTCC CAGCTTGATA AGGGTGAAAA TTCCTTCCTA 
CAGGCGACCG TGGCAAAATT GGGACCGCTC CCTCGAGTTC TTGCTGATAT TACCAAGTCT 
CTAACTAATC CTACACCAAT ACAACAGCAG CTGAGACGCT TCACTGAGCA TAACTCCAGT 
CCAAATGTCA GTGGAAGCCT CTCCTCTGGG CTGCAGAAAA TATTTGAAGA CCCCACTGAC 
AGTGATTTGC ATAAACTAAA ATCTCCAAGC CAGGACAACA CAGATGGCTA TTTCAGAGGG 
AAAACGTTAT TGCTGGTTCA GCAGGCCTCC TCCCAGAGCA TGACCTATTC TGAAAAGGAT 
GAAAAGGAAA GTAGCCTTCC TAACGGTCGG AGCATCTCCC TCATGGACCT TCAAGACACT 
CACGCTGCTC AAGTAGAGCA TGCATCTATC ATGCTCGATG TGCCTATGCG CTTAACTGGA 
AGCCAGCTTT CCATAACCCA GGTGGCCAGT ATCAAACAAC TGCGGGAAAC CCAGAGCACT 
CCCCAGAGTG CACCCCAAGT GCGAAGGCCC TTGCACCCAG CCTTGAACCA GCCAGGCAGC 
CTCCAGCCCC TGTCATTCCA GAACCCTGTC TATCACCTCA ATAACCCAAT CCCAGCAATG 
CCAAAGGCCT CTGTGGATTC TAGTTTGGAG AATCTAAGCA CTGCCAGTTC CAGAAGCCAA 
AGTAACAGTG AAGATTTCAA ACTCAGTGGG CCCAGCAATA GCAGCATGGA AGATTTCACT 
AAACGTAGCA CGCAGAGCGA GGATTTCTCC AGGCGGCACA CTGTACCAGA CAGACACATA 
CCTCTTGCTC TGCCGCGGCA GAATAGTACC GGGCAGGCCC AGATCCGGAA AATGGACCAG 
GCTGGGTTAG GTGCCCGAGC CAAAGCCCCG CCGTCCTTGC CACACAGTGC TTCCTTACGT 
AGCACGGGGA GCATGTCGGT GGCCTCTGCG GCCCTGGTGG CTGAACCTGT GCAGAATGGG 
AGCCGGTCCC GGCAGCAGTC CTCTTCCTCC AGAGAGAGCC CCGTTCCCAA AGTGAGAGCG 
ATCCAGAGAC AACAGACACA GCAGGTGCAG TCACCTGTGG ACTCTGCCAC CATGTCCCCA 
GTAGAGAGGA CAGCAGCCTG GGTGTTGAAC AATGGACAGT ATGAAGAGGA TGTGGAAGAG 
ACTGAGCAAA ATCCAGATGA ATCCAAGCAT GCTGAGAAGT ATGAGCAGGA AATTACTAAA 
CTGAAGGAGC GCCTGAGAGT GTCCAGCCGG CGCCTTGAGG AATATGAGCG CCGCTTGCTG 
GTGCAGGAGC AGCAGATGCA GAAGCTGCTG CTGGAATACA AGGCCAGGCT GGAGGACAGC 
GAGGAGCGCC TGCGCAGGCA GCAGGAGGAG AAGGACAGCC AGATGAAAAG CATCATCAGC 
AGGCTGATGG CTGTGGAAGA GGAACTGAAG AAGGATCATG CTGAGATGCA AGCAGTTATT 
GATGCAAAGC AAAAAATAAT CGATGCACAG GTCATAAATG GAGATGAGAT TATTCAAACA 
GGAAAAACGA ATCGTGTCCC TGGATTCGGC CAACACCAGA CTGATGAGCG CACTGACCCA 
AGTGAAGGAG CGGTACAGCA TGCAGGTCCG CAATGGCATC TCCCCCACCA ACCCCACCAA 
GCTTTCCATC ACGGAGAATG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013970631.1
CDS	104..3727
Translation	MKWTCNILNVAVGSQLQIPLLSINAVAMRVIVFCMEKFSGWRMSLLSPGDVAEWLDSSTEEEKSLLSFLEVPAERSPRRRSISGTSTSEKPNSMDTVNTSPFKVPGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNADDRTRGLPKLKESRSHESLLSPCSAVECLDLGRGEPVSVKPLHSSILGQDFCFEVTYLSGSKCFSCNSASERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYSLPPLHSITVHIYKDVEKKKKKDKNNYVGLVNIPTASVTGRQFVEKWYPVSTPTPNKGKTGGPSIRIKSRFQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEELACALVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTIATKSIEEYLKLVGQQYLHDALGEFIKALYESDENCEVDPSKCSSSELIDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCLNRGKQDISERLISASLFLRFLCPAIMSPSLFNLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNTPGFDGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVAKLGPLPRVLADITKSLTNPTPIQQQLRRFTEHNSSPNVSGSLSSGLQKIFEDPTDSDLHKLKSPSQDNTDGYFRGKTLLLVQQASSQSMTYSEKDEKESSLPNGRSISLMDLQDTHAAQVEHASIMLDVPMRLTGSQLSITQVASIKQLRETQSTPQSAPQVRRPLHPALNQPGSLQPLSFQNPVYHLNNPIPAMPKASVDSSLENLSTASSRSQSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTVPDRHIPLALPRQNSTGQAQIRKMDQAGLGARAKAPPSLPHSASLRSTGSMSVASAALVAEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEDVEETEQNPDESKHAEKYEQEITKLKERLRVSSRRLEEYERRLLVQEQQMQKLLLEYKARLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQVINGDEIIQTGKTNRVPGFGQHQTDERTDPSEGAVQHAGPQWHLPHQPHQAFHHGEW

Target ORF information:

RefSeq Version	XM_014115156.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014115156.1

ORF Insert Sequence:

ATGAAGTGGA CTTGTAATAT CCTTAATGTT GCTGTTGGCA GTCAGTTACA GATTCCATTA 
TTGTCGATCA ATGCCGTCGC CATGCGAGTA ATTGTGTTCT GTATGGAAAA GTTTTCTGGC 
TGGAGAATGA GTCTCCTTTC CCCCGGTGAT GTGGCCGAGT GGCTGGATAG TAGCACGGAA 
GAGGAGAAGA GCCTTCTGTC ATTTCTGGAG GTGCCGGCAG AAAGGTCCCC ACGCAGACGG 
AGTATCTCAG GGACCAGTAC ATCAGAGAAA CCCAACTCCA TGGACACTGT AAATACCTCA 
CCCTTCAAAG TACCAGGGTT CTTCAGCAAG CGCCTGAAAG GCTCCATCAA GAGGACCAAA 
AGCCAATCAA AGCTTGACAG AAACACGAGC TTTCGGCTAC CATCCCTTCG TAATGCAGAT 
GACAGGACTC GCGGTCTGCC TAAACTAAAA GAGTCACGTT CCCATGAGTC ATTGCTGAGC 
CCTTGTAGTG CAGTGGAATG TCTGGATCTT GGCAGAGGAG AGCCTGTGTC AGTGAAGCCA 
CTCCATAGCA GCATCCTTGG ACAAGACTTC TGCTTTGAGG TTACGTACTT AAGTGGAAGT 
AAATGCTTCA GCTGTAACTC TGCTTCAGAG AGAGATAAGT GGATGGAGAA CCTTCGTAGG 
ACAGTTCAAC CAAATAAGGA CAATTGCAGA CGAGCTGAAA ACGTTCTTCG TTTATGGATC 
ATTGAAGCCA AGGACCTTGC CCCCAAAAAG AAATATTTCT GTGAACTGTG CCTTGATGAT 
ACCCTCTTTG CTCGTACAAC CAGCAAGACC AAAGCAGATA ATATTTTCTG GGGTGAACAT 
TTTGAATTTT ACAGCCTTCC ACCTCTTCAT AGCATCACAG TTCACATTTA TAAAGATGTG 
GAAAAAAAGA AAAAAAAAGA CAAGAATAAT TATGTAGGGC TAGTCAACAT TCCCACTGCC 
AGTGTGACTG GGCGCCAATT TGTAGAAAAG TGGTATCCAG TGAGTACCCC TACACCCAAC 
AAAGGAAAGA CCGGAGGACC TTCTATTCGG ATTAAATCAC GTTTCCAAAC TATCACCATT 
CTGCCTATGG AGCAATACAA AGAATTTGCA GAATTTGTCA CCAGCAACTA TACCATGCTG 
TGTTCTGTCC TTGAACCAGT AATTAGTGTG AGAAATAAAG AGGAGTTGGC CTGTGCCCTA 
GTGCACATTC TTCAAAGTAC TGGCAGAGCC AAGGATTTTC TGACCGACTT GGTGATGTCT 
GAGGTGGATC GCTGTGGAGA GCATGACGTC TTGATCTTCA GAGAGAACAC CATTGCTACC 
AAATCCATTG AGGAATACCT CAAGTTGGTG GGACAACAGT ATCTTCATGA CGCATTGGGG 
GAGTTCATCA AAGCTTTGTA CGAGTCAGAT GAGAACTGTG AAGTGGATCC CAGCAAGTGT 
TCCTCTAGTG AACTGATAGA CCATCAGAGC AACCTGAAGA TGTGCTGTGA GCTGGCTTTC 
TGCAAGATCA TCAACTCTTA CTGTGTTTTC CCTCGTGAGT TGAAAGAAGT GTTTGCATCG 
TGGAAGCAGC AGTGCCTGAA CCGTGGCAAA CAGGACATCA GCGAGCGGCT CATCAGTGCC 
TCATTGTTTC TCCGTTTCCT GTGCCCTGCC ATCATGTCTC CCAGTCTCTT CAACCTTATG 
CAAGAGTACC CTGATGACCG TACATCTCGC ACCCTGACTC TGATTGCCAA GGTCATTCAG 
AACCTGGCTA ACTTTGCCAA GTTTGGTAAC AAAGAAGAAT ATATGGCATT CATGAATGAT 
TTTTTAGAAC ATGAATGGGG TGGAATGAAG CGCTTTCTTT TGGAAATCTC TAATCCGGAC 
ACCATCTCAA ACACCCCAGG CTTTGATGGT TACATTGATC TGGGCCGAGA GCTTTCAGTC 
TTGCATTCCT TACTATGGGA AGTAGTTTCC CAGCTTGATA AGGGTGAAAA TTCCTTCCTA 
CAGGCGACCG TGGCAAAATT GGGACCGCTC CCTCGAGTTC TTGCTGATAT TACCAAGTCT 
CTAACTAATC CTACACCAAT ACAACAGCAG CTGAGACGCT TCACTGAGCA TAACTCCAGT 
CCAAATGTCA GTGGAAGCCT CTCCTCTGGG CTGCAGAAAA TATTTGAAGA CCCCACTGAC 
AGTGATTTGC ATAAACTAAA ATCTCCAAGC CAGGACAACA CAGATGGCTA TTTCAGAGGG 
AAAACGTTAT TGCTGGTTCA GCAGGCCTCC TCCCAGAGCA TGACCTATTC TGAAAAGGAT 
GAAAAGGAAA GTAGCCTTCC TAACGGTCGG AGCATCTCCC TCATGGACCT TCAAGACACT 
CACGCTGCTC AAGTAGAGCA TGCATCTATC ATGCTCGATG TGCCTATGCG CTTAACTGGA 
AGCCAGCTTT CCATAACCCA GGTGGCCAGT ATCAAACAAC TGCGGGAAAC CCAGAGCACT 
CCCCAGAGTG CACCCCAAGT GCGAAGGCCC TTGCACCCAG CCTTGAACCA GCCAGGCAGC 
CTCCAGCCCC TGTCATTCCA GAACCCTGTC TATCACCTCA ATAACCCAAT CCCAGCAATG 
CCAAAGGCCT CTGTGGATTC TAGTTTGGAG AATCTAAGCA CTGCCAGTTC CAGAAGCCAA 
AGTAACAGTG AAGATTTCAA ACTCAGTGGG CCCAGCAATA GCAGCATGGA AGATTTCACT 
AAACGTAGCA CGCAGAGCGA GGATTTCTCC AGGCGGCACA CTGTACCAGA CAGACACATA 
CCTCTTGCTC TGCCGCGGCA GAATAGTACC GGGCAGGCCC AGATCCGGAA AATGGACCAG 
GCTGGGTTAG GTGCCCGAGC CAAAGCCCCG CCGTCCTTGC CACACAGTGC TTCCTTACGT 
AGCACGGGGA GCATGTCGGT GGCCTCTGCG GCCCTGGTGG CTGAACCTGT GCAGAATGGG 
AGCCGGTCCC GGCAGCAGTC CTCTTCCTCC AGAGAGAGCC CCGTTCCCAA AGTGAGAGCG 
ATCCAGAGAC AACAGACACA GCAGGTGCAG TCACCTGTGG ACTCTGCCAC CATGTCCCCA 
GTAGAGAGGA CAGCAGCCTG GGTGTTGAAC AATGGACAGT ATGAAGAGGA TGTGGAAGAG 
ACTGAGCAAA ATCCAGATGA ATCCAAGCAT GCTGAGAAGT ATGAGCAGGA AATTACTAAA 
CTGAAGGAGC GCCTGAGAGT GTCCAGCCGG CGCCTTGAGG AATATGAGCG CCGCTTGCTG 
GTGCAGGAGC AGCAGATGCA GAAGCTGCTG CTGGAATACA AGGCCAGGCT GGAGGACAGC 
GAGGAGCGCC TGCGCAGGCA GCAGGAGGAG AAGGACAGCC AGATGAAAAG CATCATCAGC 
AGGCTGATGG CTGTGGAAGA GGAACTGAAG AAGGATCATG CTGAGATGCA AGCAGTTATT 
GATGCAAAGC AAAAAATAAT CGATGCACAG GTCATAAATG GAGATGAGAT TATTCAAACA 
GGAAAAACGA ATCGTGTCCC TGGATTCGGC CAACACCAGA CTGATGAGCG CACTGACCCA 
AGTGAAGGAG CGGTACAGCA TGCAGGTCCG CAATGGCATC TCCCCCACCA ACCCCACCAA 
GCTTTCCATC ACGGAGAATG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_014115157.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013970632.1 Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X8, mRNA.

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CloneID	OCe37439
Clone ID Related Accession (Same CDS sequence)	XM_014115157.1
Accession Version	XM_014115157.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	ras GTPase-activating protein nGAP isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTGGA CTTTAATTGT GTTCTGTATG GAAAAGTTTT CTGGCTGGAG AATGAGTCTC 
CTTTCCCCCG GTGATGTGGC CGAGTGGCTG GATAGTAGCA CGGAAGAGGA GAAGAGCCTT 
CTGTCATTTC TGGAGGTGCC GGCAGAAAGG TCCCCACGCA GACGGAGTAT CTCAGGGACC 
AGTACATCAG AGAAACCCAA CTCCATGGAC ACTGTAAATA CCTCACCCTT CAAAGTACCA 
GGGTTCTTCA GCAAGCGCCT GAAAGGCTCC ATCAAGAGGA CCAAAAGCCA ATCAAAGCTT 
GACAGAAACA CGAGCTTTCG GCTACCATCC CTTCGTAATG CAGATGACAG GACTCGCGGT 
CTGCCTAAAC TAAAAGAGTC ACGTTCCCAT GAGTCATTGC TGAGCCCTTG TAGTGCAGTG 
GAATGTCTGG ATCTTGGCAG AGGAGAGCCT GTGTCAGTGA AGCCACTCCA TAGCAGCATC 
CTTGGACAAG ACTTCTGCTT TGAGGTTACG TACTTAAGTG GAAGTAAATG CTTCAGCTGT 
AACTCTGCTT CAGAGAGAGA TAAGTGGATG GAGAACCTTC GTAGGACAGT TCAACCAAAT 
AAGGACAATT GCAGACGAGC TGAAAACGTT CTTCGTTTAT GGATCATTGA AGCCAAGGAC 
CTTGCCCCCA AAAAGAAATA TTTCTGTGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT 
ACAACCAGCA AGACCAAAGC AGATAATATT TTCTGGGGTG AACATTTTGA ATTTTACAGC 
CTTCCACCTC TTCATAGCAT CACAGTTCAC ATTTATAAAG ATGTGGAAAA AAAGAAAAAA 
AAAGACAAGA ATAATTATGT AGGGCTAGTC AACATTCCCA CTGCCAGTGT GACTGGGCGC 
CAATTTGTAG AAAAGTGGTA TCCAGTGAGT ACCCCTACAC CCAACAAAGG AAAGACCGGA 
GGACCTTCTA TTCGGATTAA ATCACGTTTC CAAACTATCA CCATTCTGCC TATGGAGCAA 
TACAAAGAAT TTGCAGAATT TGTCACCAGC AACTATACCA TGCTGTGTTC TGTCCTTGAA 
CCAGTAATTA GTGTGAGAAA TAAAGAGGAG TTGGCCTGTG CCCTAGTGCA CATTCTTCAA 
AGTACTGGCA GAGCCAAGGA TTTTCTGACC GACTTGGTGA TGTCTGAGGT GGATCGCTGT 
GGAGAGCATG ACGTCTTGAT CTTCAGAGAG AACACCATTG CTACCAAATC CATTGAGGAA 
TACCTCAAGT TGGTGGGACA ACAGTATCTT CATGACGCAT TGGGGGAGTT CATCAAAGCT 
TTGTACGAGT CAGATGAGAA CTGTGAAGTG GATCCCAGCA AGTGTTCCTC TAGTGAACTG 
ATAGACCATC AGAGCAACCT GAAGATGTGC TGTGAGCTGG CTTTCTGCAA GATCATCAAC 
TCTTACTGTG TTTTCCCTCG TGAGTTGAAA GAAGTGTTTG CATCGTGGAA GCAGCAGTGC 
CTGAACCGTG GCAAACAGGA CATCAGCGAG CGGCTCATCA GTGCCTCATT GTTTCTCCGT 
TTCCTGTGCC CTGCCATCAT GTCTCCCAGT CTCTTCAACC TTATGCAAGA GTACCCTGAT 
GACCGTACAT CTCGCACCCT GACTCTGATT GCCAAGGTCA TTCAGAACCT GGCTAACTTT 
GCCAAGTTTG GTAACAAAGA AGAATATATG GCATTCATGA ATGATTTTTT AGAACATGAA 
TGGGGTGGAA TGAAGCGCTT TCTTTTGGAA ATCTCTAATC CGGACACCAT CTCAAACACC 
CCAGGCTTTG ATGGTTACAT TGATCTGGGC CGAGAGCTTT CAGTCTTGCA TTCCTTACTA 
TGGGAAGTAG TTTCCCAGCT TGATAAGGGT GAAAATTCCT TCCTACAGGC GACCGTGGCA 
AAATTGGGAC CGCTCCCTCG AGTTCTTGCT GATATTACCA AGTCTCTAAC TAATCCTACA 
CCAATACAAC AGCAGCTGAG ACGCTTCACT GAGCATAACT CCAGTCCAAA TGTCAGTGGA 
AGCCTCTCCT CTGGGCTGCA GAAAATATTT GAAGACCCCA CTGACAGTGA TTTGCATAAA 
CTAAAATCTC CAAGCCAGGA CAACACAGAT GGCTATTTCA GAGGGAAAAC GTTATTGCTG 
GTTCAGCAGG CCTCCTCCCA GAGCATGACC TATTCTGAAA AGGATGAAAA GGAAAGTAGC 
CTTCCTAACG GTCGGAGCAT CTCCCTCATG GACCTTCAAG ACACTCACGC TGCTCAAGTA 
GAGCATGCAT CTATCATGCT CGATGTGCCT ATGCGCTTAA CTGGAAGCCA GCTTTCCATA 
ACCCAGGTGG CCAGTATCAA ACAACTGCGG GAAACCCAGA GCACTCCCCA GAGTGCACCC 
CAAGTGCGAA GGCCCTTGCA CCCAGCCTTG AACCAGCCAG GCAGCCTCCA GCCCCTGTCA 
TTCCAGAACC CTGTCTATCA CCTCAATAAC CCAATCCCAG CAATGCCAAA GGCCTCTGTG 
GATTCTAGTT TGGAGAATCT AAGCACTGCC AGTTCCAGAA GCCAAAGTAA CAGTGAAGAT 
TTCAAACTCA GTGGGCCCAG CAATAGCAGC ATGGAAGATT TCACTAAACG TAGCACGCAG 
AGCGAGGATT TCTCCAGGCG GCACACTGTA CCAGACAGAC ACATACCTCT TGCTCTGCCG 
CGGCAGAATA GTACCGGGCA GGCCCAGATC CGGAAAATGG ACCAGGCTGG GTTAGGTGCC 
CGAGCCAAAG CCCCGCCGTC CTTGCCACAC AGTGCTTCCT TACGTAGCAC GGGGAGCATG 
TCGGTGGCCT CTGCGGCCCT GGTGGCTGAA CCTGTGCAGA ATGGGAGCCG GTCCCGGCAG 
CAGTCCTCTT CCTCCAGAGA GAGCCCCGTT CCCAAAGTGA GAGCGATCCA GAGACAACAG 
ACACAGCAGG TGCAGTCACC TGTGGACTCT GCCACCATGT CCCCAGTAGA GAGGACAGCA 
GCCTGGGTGT TGAACAATGG ACAGTATGAA GAGGATGTGG AAGAGACTGA GCAAAATCCA 
GATGAATCCA AGCATGCTGA GAAGTATGAG CAGGAAATTA CTAAACTGAA GGAGCGCCTG 
AGAGTGTCCA GCCGGCGCCT TGAGGAATAT GAGCGCCGCT TGCTGGTGCA GGAGCAGCAG 
ATGCAGAAGC TGCTGCTGGA ATACAAGGCC AGGCTGGAGG ACAGCGAGGA GCGCCTGCGC 
AGGCAGCAGG AGGAGAAGGA CAGCCAGATG AAAAGCATCA TCAGCAGGCT GATGGCTGTG 
GAAGAGGAAC TGAAGAAGGA TCATGCTGAG ATGCAAGCAG TTATTGATGC AAAGCAAAAA 
ATAATCGATG CACAGGTCAT AAATGGAGAT GAGATTATTC AAACAGGAAA AACGAATCGT 
GTCCCTGGAT TCGGCCAACA CCAGACTGAT GAGCGCACTG ACCCAAGTGA AGGAGCGGTA 
CAGCATGCAG GTCCGCAATG GCATCTCCCC CACCAACCCC ACCAAGCTTT CCATCACGGA 
GAATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013970632.1
CDS	103..3651
Translation	MKWTLIVFCMEKFSGWRMSLLSPGDVAEWLDSSTEEEKSLLSFLEVPAERSPRRRSISGTSTSEKPNSMDTVNTSPFKVPGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNADDRTRGLPKLKESRSHESLLSPCSAVECLDLGRGEPVSVKPLHSSILGQDFCFEVTYLSGSKCFSCNSASERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYSLPPLHSITVHIYKDVEKKKKKDKNNYVGLVNIPTASVTGRQFVEKWYPVSTPTPNKGKTGGPSIRIKSRFQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEELACALVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTIATKSIEEYLKLVGQQYLHDALGEFIKALYESDENCEVDPSKCSSSELIDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCLNRGKQDISERLISASLFLRFLCPAIMSPSLFNLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNTPGFDGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVAKLGPLPRVLADITKSLTNPTPIQQQLRRFTEHNSSPNVSGSLSSGLQKIFEDPTDSDLHKLKSPSQDNTDGYFRGKTLLLVQQASSQSMTYSEKDEKESSLPNGRSISLMDLQDTHAAQVEHASIMLDVPMRLTGSQLSITQVASIKQLRETQSTPQSAPQVRRPLHPALNQPGSLQPLSFQNPVYHLNNPIPAMPKASVDSSLENLSTASSRSQSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTVPDRHIPLALPRQNSTGQAQIRKMDQAGLGARAKAPPSLPHSASLRSTGSMSVASAALVAEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEDVEETEQNPDESKHAEKYEQEITKLKERLRVSSRRLEEYERRLLVQEQQMQKLLLEYKARLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQVINGDEIIQTGKTNRVPGFGQHQTDERTDPSEGAVQHAGPQWHLPHQPHQAFHHGEW

Target ORF information:

RefSeq Version	XM_014115157.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014115157.1

ORF Insert Sequence:

ATGAAGTGGA CTTTAATTGT GTTCTGTATG GAAAAGTTTT CTGGCTGGAG AATGAGTCTC 
CTTTCCCCCG GTGATGTGGC CGAGTGGCTG GATAGTAGCA CGGAAGAGGA GAAGAGCCTT 
CTGTCATTTC TGGAGGTGCC GGCAGAAAGG TCCCCACGCA GACGGAGTAT CTCAGGGACC 
AGTACATCAG AGAAACCCAA CTCCATGGAC ACTGTAAATA CCTCACCCTT CAAAGTACCA 
GGGTTCTTCA GCAAGCGCCT GAAAGGCTCC ATCAAGAGGA CCAAAAGCCA ATCAAAGCTT 
GACAGAAACA CGAGCTTTCG GCTACCATCC CTTCGTAATG CAGATGACAG GACTCGCGGT 
CTGCCTAAAC TAAAAGAGTC ACGTTCCCAT GAGTCATTGC TGAGCCCTTG TAGTGCAGTG 
GAATGTCTGG ATCTTGGCAG AGGAGAGCCT GTGTCAGTGA AGCCACTCCA TAGCAGCATC 
CTTGGACAAG ACTTCTGCTT TGAGGTTACG TACTTAAGTG GAAGTAAATG CTTCAGCTGT 
AACTCTGCTT CAGAGAGAGA TAAGTGGATG GAGAACCTTC GTAGGACAGT TCAACCAAAT 
AAGGACAATT GCAGACGAGC TGAAAACGTT CTTCGTTTAT GGATCATTGA AGCCAAGGAC 
CTTGCCCCCA AAAAGAAATA TTTCTGTGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT 
ACAACCAGCA AGACCAAAGC AGATAATATT TTCTGGGGTG AACATTTTGA ATTTTACAGC 
CTTCCACCTC TTCATAGCAT CACAGTTCAC ATTTATAAAG ATGTGGAAAA AAAGAAAAAA 
AAAGACAAGA ATAATTATGT AGGGCTAGTC AACATTCCCA CTGCCAGTGT GACTGGGCGC 
CAATTTGTAG AAAAGTGGTA TCCAGTGAGT ACCCCTACAC CCAACAAAGG AAAGACCGGA 
GGACCTTCTA TTCGGATTAA ATCACGTTTC CAAACTATCA CCATTCTGCC TATGGAGCAA 
TACAAAGAAT TTGCAGAATT TGTCACCAGC AACTATACCA TGCTGTGTTC TGTCCTTGAA 
CCAGTAATTA GTGTGAGAAA TAAAGAGGAG TTGGCCTGTG CCCTAGTGCA CATTCTTCAA 
AGTACTGGCA GAGCCAAGGA TTTTCTGACC GACTTGGTGA TGTCTGAGGT GGATCGCTGT 
GGAGAGCATG ACGTCTTGAT CTTCAGAGAG AACACCATTG CTACCAAATC CATTGAGGAA 
TACCTCAAGT TGGTGGGACA ACAGTATCTT CATGACGCAT TGGGGGAGTT CATCAAAGCT 
TTGTACGAGT CAGATGAGAA CTGTGAAGTG GATCCCAGCA AGTGTTCCTC TAGTGAACTG 
ATAGACCATC AGAGCAACCT GAAGATGTGC TGTGAGCTGG CTTTCTGCAA GATCATCAAC 
TCTTACTGTG TTTTCCCTCG TGAGTTGAAA GAAGTGTTTG CATCGTGGAA GCAGCAGTGC 
CTGAACCGTG GCAAACAGGA CATCAGCGAG CGGCTCATCA GTGCCTCATT GTTTCTCCGT 
TTCCTGTGCC CTGCCATCAT GTCTCCCAGT CTCTTCAACC TTATGCAAGA GTACCCTGAT 
GACCGTACAT CTCGCACCCT GACTCTGATT GCCAAGGTCA TTCAGAACCT GGCTAACTTT 
GCCAAGTTTG GTAACAAAGA AGAATATATG GCATTCATGA ATGATTTTTT AGAACATGAA 
TGGGGTGGAA TGAAGCGCTT TCTTTTGGAA ATCTCTAATC CGGACACCAT CTCAAACACC 
CCAGGCTTTG ATGGTTACAT TGATCTGGGC CGAGAGCTTT CAGTCTTGCA TTCCTTACTA 
TGGGAAGTAG TTTCCCAGCT TGATAAGGGT GAAAATTCCT TCCTACAGGC GACCGTGGCA 
AAATTGGGAC CGCTCCCTCG AGTTCTTGCT GATATTACCA AGTCTCTAAC TAATCCTACA 
CCAATACAAC AGCAGCTGAG ACGCTTCACT GAGCATAACT CCAGTCCAAA TGTCAGTGGA 
AGCCTCTCCT CTGGGCTGCA GAAAATATTT GAAGACCCCA CTGACAGTGA TTTGCATAAA 
CTAAAATCTC CAAGCCAGGA CAACACAGAT GGCTATTTCA GAGGGAAAAC GTTATTGCTG 
GTTCAGCAGG CCTCCTCCCA GAGCATGACC TATTCTGAAA AGGATGAAAA GGAAAGTAGC 
CTTCCTAACG GTCGGAGCAT CTCCCTCATG GACCTTCAAG ACACTCACGC TGCTCAAGTA 
GAGCATGCAT CTATCATGCT CGATGTGCCT ATGCGCTTAA CTGGAAGCCA GCTTTCCATA 
ACCCAGGTGG CCAGTATCAA ACAACTGCGG GAAACCCAGA GCACTCCCCA GAGTGCACCC 
CAAGTGCGAA GGCCCTTGCA CCCAGCCTTG AACCAGCCAG GCAGCCTCCA GCCCCTGTCA 
TTCCAGAACC CTGTCTATCA CCTCAATAAC CCAATCCCAG CAATGCCAAA GGCCTCTGTG 
GATTCTAGTT TGGAGAATCT AAGCACTGCC AGTTCCAGAA GCCAAAGTAA CAGTGAAGAT 
TTCAAACTCA GTGGGCCCAG CAATAGCAGC ATGGAAGATT TCACTAAACG TAGCACGCAG 
AGCGAGGATT TCTCCAGGCG GCACACTGTA CCAGACAGAC ACATACCTCT TGCTCTGCCG 
CGGCAGAATA GTACCGGGCA GGCCCAGATC CGGAAAATGG ACCAGGCTGG GTTAGGTGCC 
CGAGCCAAAG CCCCGCCGTC CTTGCCACAC AGTGCTTCCT TACGTAGCAC GGGGAGCATG 
TCGGTGGCCT CTGCGGCCCT GGTGGCTGAA CCTGTGCAGA ATGGGAGCCG GTCCCGGCAG 
CAGTCCTCTT CCTCCAGAGA GAGCCCCGTT CCCAAAGTGA GAGCGATCCA GAGACAACAG 
ACACAGCAGG TGCAGTCACC TGTGGACTCT GCCACCATGT CCCCAGTAGA GAGGACAGCA 
GCCTGGGTGT TGAACAATGG ACAGTATGAA GAGGATGTGG AAGAGACTGA GCAAAATCCA 
GATGAATCCA AGCATGCTGA GAAGTATGAG CAGGAAATTA CTAAACTGAA GGAGCGCCTG 
AGAGTGTCCA GCCGGCGCCT TGAGGAATAT GAGCGCCGCT TGCTGGTGCA GGAGCAGCAG 
ATGCAGAAGC TGCTGCTGGA ATACAAGGCC AGGCTGGAGG ACAGCGAGGA GCGCCTGCGC 
AGGCAGCAGG AGGAGAAGGA CAGCCAGATG AAAAGCATCA TCAGCAGGCT GATGGCTGTG 
GAAGAGGAAC TGAAGAAGGA TCATGCTGAG ATGCAAGCAG TTATTGATGC AAAGCAAAAA 
ATAATCGATG CACAGGTCAT AAATGGAGAT GAGATTATTC AAACAGGAAA AACGAATCGT 
GTCCCTGGAT TCGGCCAACA CCAGACTGAT GAGCGCACTG ACCCAAGTGA AGGAGCGGTA 
CAGCATGCAG GTCCGCAATG GCATCTCCCC CACCAACCCC ACCAAGCTTT CCATCACGGA 
GAATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_014115158.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013970633.1 Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OCe37440
Clone ID Related Accession (Same CDS sequence)	XM_014115158.1 , XM_014115158.2
Accession Version	XM_014115158.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	ras GTPase-activating protein nGAP isoform X9
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAGCA GTTGTGATAA AGAGGTGCCG GCAGAAAGGT CCCCACGCAG ACGGAGTATC 
TCAGGGACCA GTACATCAGA GAAACCCAAC TCCATGGACA CTGTAAATAC CTCACCCTTC 
AAAGTACCAG GGTTCTTCAG CAAGCGCCTG AAAGGCTCCA TCAAGAGGAC CAAAAGCCAA 
TCAAAGCTTG ACAGAAACAC GAGCTTTCGG CTACCATCCC TTCGTAATGC AGATGACAGG 
ACTCGCGGTC TGCCTAAACT AAAAGAGTCA CGTTCCCATG AGTCATTGCT GAGCCCTTGT 
AGTGCAGTGG AATGTCTGGA TCTTGGCAGA GGAGAGCCTG TGTCAGTGAA GCCACTCCAT 
AGCAGCATCC TTGGACAAGA CTTCTGCTTT GAGGTTACGT ACTTAAGTGG AAGTAAATGC 
TTCAGCTGTA ACTCTGCTTC AGAGAGAGAT AAGTGGATGG AGAACCTTCG TAGGACAGTT 
CAACCAAATA AGGACAATTG CAGACGAGCT GAAAACGTTC TTCGTTTATG GATCATTGAA 
GCCAAGGACC TTGCCCCCAA AAAGAAATAT TTCTGTGAAC TGTGCCTTGA TGATACCCTC 
TTTGCTCGTA CAACCAGCAA GACCAAAGCA GATAATATTT TCTGGGGTGA ACATTTTGAA 
TTTTACAGCC TTCCACCTCT TCATAGCATC ACAGTTCACA TTTATAAAGA TGTGGAAAAA 
AAGAAAAAAA AAGACAAGAA TAATTATGTA GGGCTAGTCA ACATTCCCAC TGCCAGTGTG 
ACTGGGCGCC AATTTGTAGA AAAGTGGTAT CCAGTGAGTA CCCCTACACC CAACAAAGGA 
AAGACCGGAG GACCTTCTAT TCGGATTAAA TCACGTTTCC AAACTATCAC CATTCTGCCT 
ATGGAGCAAT ACAAAGAATT TGCAGAATTT GTCACCAGCA ACTATACCAT GCTGTGTTCT 
GTCCTTGAAC CAGTAATTAG TGTGAGAAAT AAAGAGGAGT TGGCCTGTGC CCTAGTGCAC 
ATTCTTCAAA GTACTGGCAG AGCCAAGGAT TTTCTGACCG ACTTGGTGAT GTCTGAGGTG 
GATCGCTGTG GAGAGCATGA CGTCTTGATC TTCAGAGAGA ACACCATTGC TACCAAATCC 
ATTGAGGAAT ACCTCAAGTT GGTGGGACAA CAGTATCTTC ATGACGCATT GGGGGAGTTC 
ATCAAAGCTT TGTACGAGTC AGATGAGAAC TGTGAAGTGG ATCCCAGCAA GTGTTCCTCT 
AGTGAACTGA TAGACCATCA GAGCAACCTG AAGATGTGCT GTGAGCTGGC TTTCTGCAAG 
ATCATCAACT CTTACTGTGT TTTCCCTCGT GAGTTGAAAG AAGTGTTTGC ATCGTGGAAG 
CAGCAGTGCC TGAACCGTGG CAAACAGGAC ATCAGCGAGC GGCTCATCAG TGCCTCATTG 
TTTCTCCGTT TCCTGTGCCC TGCCATCATG TCTCCCAGTC TCTTCAACCT TATGCAAGAG 
TACCCTGATG ACCGTACATC TCGCACCCTG ACTCTGATTG CCAAGGTCAT TCAGAACCTG 
GCTAACTTTG CCAAGTTTGG TAACAAAGAA GAATATATGG CATTCATGAA TGATTTTTTA 
GAACATGAAT GGGGTGGAAT GAAGCGCTTT CTTTTGGAAA TCTCTAATCC GGACACCATC 
TCAAACACCC CAGGCTTTGA TGGTTACATT GATCTGGGCC GAGAGCTTTC AGTCTTGCAT 
TCCTTACTAT GGGAAGTAGT TTCCCAGCTT GATAAGGGTG AAAATTCCTT CCTACAGGCG 
ACCGTGGCAA AATTGGGACC GCTCCCTCGA GTTCTTGCTG ATATTACCAA GTCTCTAACT 
AATCCTACAC CAATACAACA GCAGCTGAGA CGCTTCACTG AGCATAACTC CAGTCCAAAT 
GTCAGTGGAA GCCTCTCCTC TGGGCTGCAG AAAATATTTG AAGACCCCAC TGACAGTGAT 
TTGCATAAAC TAAAATCTCC AAGCCAGGAC AACACAGATG GCTATTTCAG AGGGAAAACG 
TTATTGCTGG TTCAGCAGGC CTCCTCCCAG AGCATGACCT ATTCTGAAAA GGATGAAAAG 
GAAAGTAGCC TTCCTAACGG TCGGAGCATC TCCCTCATGG ACCTTCAAGA CACTCACGCT 
GCTCAAGTAG AGCATGCATC TATCATGCTC GATGTGCCTA TGCGCTTAAC TGGAAGCCAG 
CTTTCCATAA CCCAGGTGGC CAGTATCAAA CAACTGCGGG AAACCCAGAG CACTCCCCAG 
AGTGCACCCC AAGTGCGAAG GCCCTTGCAC CCAGCCTTGA ACCAGCCAGG CAGCCTCCAG 
CCCCTGTCAT TCCAGAACCC TGTCTATCAC CTCAATAACC CAATCCCAGC AATGCCAAAG 
GCCTCTGTGG ATTCTAGTTT GGAGAATCTA AGCACTGCCA GTTCCAGAAG CCAAAGTAAC 
AGTGAAGATT TCAAACTCAG TGGGCCCAGC AATAGCAGCA TGGAAGATTT CACTAAACGT 
AGCACGCAGA GCGAGGATTT CTCCAGGCGG CACACTGTAC CAGACAGACA CATACCTCTT 
GCTCTGCCGC GGCAGAATAG TACCGGGCAG GCCCAGATCC GGAAAATGGA CCAGGCTGGG 
TTAGGTGCCC GAGCCAAAGC CCCGCCGTCC TTGCCACACA GTGCTTCCTT ACGTAGCACG 
GGGAGCATGT CGGTGGCCTC TGCGGCCCTG GTGGCTGAAC CTGTGCAGAA TGGGAGCCGG 
TCCCGGCAGC AGTCCTCTTC CTCCAGAGAG AGCCCCGTTC CCAAAGTGAG AGCGATCCAG 
AGACAACAGA CACAGCAGGT GCAGTCACCT GTGGACTCTG CCACCATGTC CCCAGTAGAG 
AGGACAGCAG CCTGGGTGTT GAACAATGGA CAGTATGAAG AGGATGTGGA AGAGACTGAG 
CAAAATCCAG ATGAATCCAA GCATGCTGAG AAGTATGAGC AGGAAATTAC TAAACTGAAG 
GAGCGCCTGA GAGTGTCCAG CCGGCGCCTT GAGGAATATG AGCGCCGCTT GCTGGTGCAG 
GAGCAGCAGA TGCAGAAGCT GCTGCTGGAA TACAAGGCCA GGCTGGAGGA CAGCGAGGAG 
CGCCTGCGCA GGCAGCAGGA GGAGAAGGAC AGCCAGATGA AAAGCATCAT CAGCAGGCTG 
ATGGCTGTGG AAGAGGAACT GAAGAAGGAT CATGCTGAGA TGCAAGCAGT TATTGATGCA 
AAGCAAAAAA TAATCGATGC ACAGGTCATA AATGGAGATG AGATTATTCA AACAGGAAAA 
ACGAATCGTG TCCCTGGATT CGGCCAACAC CAGACTGATG AGCGCACTGA CCCAAGTGAA 
GGAGCGGTAC AGCATGCAGG TCCGCAATGG CATCTCCCCC ACCAACCCCA CCAAGCTTTC 
CATCACGGAG AATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013970633.1
CDS	284..3721
Translation	MGSSCDKEVPAERSPRRRSISGTSTSEKPNSMDTVNTSPFKVPGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNADDRTRGLPKLKESRSHESLLSPCSAVECLDLGRGEPVSVKPLHSSILGQDFCFEVTYLSGSKCFSCNSASERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYSLPPLHSITVHIYKDVEKKKKKDKNNYVGLVNIPTASVTGRQFVEKWYPVSTPTPNKGKTGGPSIRIKSRFQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEELACALVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTIATKSIEEYLKLVGQQYLHDALGEFIKALYESDENCEVDPSKCSSSELIDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCLNRGKQDISERLISASLFLRFLCPAIMSPSLFNLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNTPGFDGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVAKLGPLPRVLADITKSLTNPTPIQQQLRRFTEHNSSPNVSGSLSSGLQKIFEDPTDSDLHKLKSPSQDNTDGYFRGKTLLLVQQASSQSMTYSEKDEKESSLPNGRSISLMDLQDTHAAQVEHASIMLDVPMRLTGSQLSITQVASIKQLRETQSTPQSAPQVRRPLHPALNQPGSLQPLSFQNPVYHLNNPIPAMPKASVDSSLENLSTASSRSQSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTVPDRHIPLALPRQNSTGQAQIRKMDQAGLGARAKAPPSLPHSASLRSTGSMSVASAALVAEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEDVEETEQNPDESKHAEKYEQEITKLKERLRVSSRRLEEYERRLLVQEQQMQKLLLEYKARLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQVINGDEIIQTGKTNRVPGFGQHQTDERTDPSEGAVQHAGPQWHLPHQPHQAFHHGEW

Target ORF information:

RefSeq Version	XM_014115158.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014115158.1

ORF Insert Sequence:

ATGGGAAGCA GTTGTGATAA AGAGGTGCCG GCAGAAAGGT CCCCACGCAG ACGGAGTATC 
TCAGGGACCA GTACATCAGA GAAACCCAAC TCCATGGACA CTGTAAATAC CTCACCCTTC 
AAAGTACCAG GGTTCTTCAG CAAGCGCCTG AAAGGCTCCA TCAAGAGGAC CAAAAGCCAA 
TCAAAGCTTG ACAGAAACAC GAGCTTTCGG CTACCATCCC TTCGTAATGC AGATGACAGG 
ACTCGCGGTC TGCCTAAACT AAAAGAGTCA CGTTCCCATG AGTCATTGCT GAGCCCTTGT 
AGTGCAGTGG AATGTCTGGA TCTTGGCAGA GGAGAGCCTG TGTCAGTGAA GCCACTCCAT 
AGCAGCATCC TTGGACAAGA CTTCTGCTTT GAGGTTACGT ACTTAAGTGG AAGTAAATGC 
TTCAGCTGTA ACTCTGCTTC AGAGAGAGAT AAGTGGATGG AGAACCTTCG TAGGACAGTT 
CAACCAAATA AGGACAATTG CAGACGAGCT GAAAACGTTC TTCGTTTATG GATCATTGAA 
GCCAAGGACC TTGCCCCCAA AAAGAAATAT TTCTGTGAAC TGTGCCTTGA TGATACCCTC 
TTTGCTCGTA CAACCAGCAA GACCAAAGCA GATAATATTT TCTGGGGTGA ACATTTTGAA 
TTTTACAGCC TTCCACCTCT TCATAGCATC ACAGTTCACA TTTATAAAGA TGTGGAAAAA 
AAGAAAAAAA AAGACAAGAA TAATTATGTA GGGCTAGTCA ACATTCCCAC TGCCAGTGTG 
ACTGGGCGCC AATTTGTAGA AAAGTGGTAT CCAGTGAGTA CCCCTACACC CAACAAAGGA 
AAGACCGGAG GACCTTCTAT TCGGATTAAA TCACGTTTCC AAACTATCAC CATTCTGCCT 
ATGGAGCAAT ACAAAGAATT TGCAGAATTT GTCACCAGCA ACTATACCAT GCTGTGTTCT 
GTCCTTGAAC CAGTAATTAG TGTGAGAAAT AAAGAGGAGT TGGCCTGTGC CCTAGTGCAC 
ATTCTTCAAA GTACTGGCAG AGCCAAGGAT TTTCTGACCG ACTTGGTGAT GTCTGAGGTG 
GATCGCTGTG GAGAGCATGA CGTCTTGATC TTCAGAGAGA ACACCATTGC TACCAAATCC 
ATTGAGGAAT ACCTCAAGTT GGTGGGACAA CAGTATCTTC ATGACGCATT GGGGGAGTTC 
ATCAAAGCTT TGTACGAGTC AGATGAGAAC TGTGAAGTGG ATCCCAGCAA GTGTTCCTCT 
AGTGAACTGA TAGACCATCA GAGCAACCTG AAGATGTGCT GTGAGCTGGC TTTCTGCAAG 
ATCATCAACT CTTACTGTGT TTTCCCTCGT GAGTTGAAAG AAGTGTTTGC ATCGTGGAAG 
CAGCAGTGCC TGAACCGTGG CAAACAGGAC ATCAGCGAGC GGCTCATCAG TGCCTCATTG 
TTTCTCCGTT TCCTGTGCCC TGCCATCATG TCTCCCAGTC TCTTCAACCT TATGCAAGAG 
TACCCTGATG ACCGTACATC TCGCACCCTG ACTCTGATTG CCAAGGTCAT TCAGAACCTG 
GCTAACTTTG CCAAGTTTGG TAACAAAGAA GAATATATGG CATTCATGAA TGATTTTTTA 
GAACATGAAT GGGGTGGAAT GAAGCGCTTT CTTTTGGAAA TCTCTAATCC GGACACCATC 
TCAAACACCC CAGGCTTTGA TGGTTACATT GATCTGGGCC GAGAGCTTTC AGTCTTGCAT 
TCCTTACTAT GGGAAGTAGT TTCCCAGCTT GATAAGGGTG AAAATTCCTT CCTACAGGCG 
ACCGTGGCAA AATTGGGACC GCTCCCTCGA GTTCTTGCTG ATATTACCAA GTCTCTAACT 
AATCCTACAC CAATACAACA GCAGCTGAGA CGCTTCACTG AGCATAACTC CAGTCCAAAT 
GTCAGTGGAA GCCTCTCCTC TGGGCTGCAG AAAATATTTG AAGACCCCAC TGACAGTGAT 
TTGCATAAAC TAAAATCTCC AAGCCAGGAC AACACAGATG GCTATTTCAG AGGGAAAACG 
TTATTGCTGG TTCAGCAGGC CTCCTCCCAG AGCATGACCT ATTCTGAAAA GGATGAAAAG 
GAAAGTAGCC TTCCTAACGG TCGGAGCATC TCCCTCATGG ACCTTCAAGA CACTCACGCT 
GCTCAAGTAG AGCATGCATC TATCATGCTC GATGTGCCTA TGCGCTTAAC TGGAAGCCAG 
CTTTCCATAA CCCAGGTGGC CAGTATCAAA CAACTGCGGG AAACCCAGAG CACTCCCCAG 
AGTGCACCCC AAGTGCGAAG GCCCTTGCAC CCAGCCTTGA ACCAGCCAGG CAGCCTCCAG 
CCCCTGTCAT TCCAGAACCC TGTCTATCAC CTCAATAACC CAATCCCAGC AATGCCAAAG 
GCCTCTGTGG ATTCTAGTTT GGAGAATCTA AGCACTGCCA GTTCCAGAAG CCAAAGTAAC 
AGTGAAGATT TCAAACTCAG TGGGCCCAGC AATAGCAGCA TGGAAGATTT CACTAAACGT 
AGCACGCAGA GCGAGGATTT CTCCAGGCGG CACACTGTAC CAGACAGACA CATACCTCTT 
GCTCTGCCGC GGCAGAATAG TACCGGGCAG GCCCAGATCC GGAAAATGGA CCAGGCTGGG 
TTAGGTGCCC GAGCCAAAGC CCCGCCGTCC TTGCCACACA GTGCTTCCTT ACGTAGCACG 
GGGAGCATGT CGGTGGCCTC TGCGGCCCTG GTGGCTGAAC CTGTGCAGAA TGGGAGCCGG 
TCCCGGCAGC AGTCCTCTTC CTCCAGAGAG AGCCCCGTTC CCAAAGTGAG AGCGATCCAG 
AGACAACAGA CACAGCAGGT GCAGTCACCT GTGGACTCTG CCACCATGTC CCCAGTAGAG 
AGGACAGCAG CCTGGGTGTT GAACAATGGA CAGTATGAAG AGGATGTGGA AGAGACTGAG 
CAAAATCCAG ATGAATCCAA GCATGCTGAG AAGTATGAGC AGGAAATTAC TAAACTGAAG 
GAGCGCCTGA GAGTGTCCAG CCGGCGCCTT GAGGAATATG AGCGCCGCTT GCTGGTGCAG 
GAGCAGCAGA TGCAGAAGCT GCTGCTGGAA TACAAGGCCA GGCTGGAGGA CAGCGAGGAG 
CGCCTGCGCA GGCAGCAGGA GGAGAAGGAC AGCCAGATGA AAAGCATCAT CAGCAGGCTG 
ATGGCTGTGG AAGAGGAACT GAAGAAGGAT CATGCTGAGA TGCAAGCAGT TATTGATGCA 
AAGCAAAAAA TAATCGATGC ACAGGTCATA AATGGAGATG AGATTATTCA AACAGGAAAA 
ACGAATCGTG TCCCTGGATT CGGCCAACAC CAGACTGATG AGCGCACTGA CCCAAGTGAA 
GGAGCGGTAC AGCATGCAGG TCCGCAATGG CATCTCCCCC ACCAACCCCA CCAAGCTTTC 
CATCACGGAG AATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_014115158.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013970633.1 Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X11, mRNA.

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CloneID	OCe37440
Clone ID Related Accession (Same CDS sequence)	XM_014115158.1 , XM_014115158.2
Accession Version	XM_014115158.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	ras GTPase-activating protein nGAP isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014115158.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAGCA GTTGTGATAA AGAGGTGCCG GCAGAAAGGT CCCCACGCAG ACGGAGTATC 
TCAGGGACCA GTACATCAGA GAAACCCAAC TCCATGGACA CTGTAAATAC CTCACCCTTC 
AAAGTACCAG GGTTCTTCAG CAAGCGCCTG AAAGGCTCCA TCAAGAGGAC CAAAAGCCAA 
TCAAAGCTTG ACAGAAACAC GAGCTTTCGG CTACCATCCC TTCGTAATGC AGATGACAGG 
ACTCGCGGTC TGCCTAAACT AAAAGAGTCA CGTTCCCATG AGTCATTGCT GAGCCCTTGT 
AGTGCAGTGG AATGTCTGGA TCTTGGCAGA GGAGAGCCTG TGTCAGTGAA GCCACTCCAT 
AGCAGCATCC TTGGACAAGA CTTCTGCTTT GAGGTTACGT ACTTAAGTGG AAGTAAATGC 
TTCAGCTGTA ACTCTGCTTC AGAGAGAGAT AAGTGGATGG AGAACCTTCG TAGGACAGTT 
CAACCAAATA AGGACAATTG CAGACGAGCT GAAAACGTTC TTCGTTTATG GATCATTGAA 
GCCAAGGACC TTGCCCCCAA AAAGAAATAT TTCTGTGAAC TGTGCCTTGA TGATACCCTC 
TTTGCTCGTA CAACCAGCAA GACCAAAGCA GATAATATTT TCTGGGGTGA ACATTTTGAA 
TTTTACAGCC TTCCACCTCT TCATAGCATC ACAGTTCACA TTTATAAAGA TGTGGAAAAA 
AAGAAAAAAA AAGACAAGAA TAATTATGTA GGGCTAGTCA ACATTCCCAC TGCCAGTGTG 
ACTGGGCGCC AATTTGTAGA AAAGTGGTAT CCAGTGAGTA CCCCTACACC CAACAAAGGA 
AAGACCGGAG GACCTTCTAT TCGGATTAAA TCACGTTTCC AAACTATCAC CATTCTGCCT 
ATGGAGCAAT ACAAAGAATT TGCAGAATTT GTCACCAGCA ACTATACCAT GCTGTGTTCT 
GTCCTTGAAC CAGTAATTAG TGTGAGAAAT AAAGAGGAGT TGGCCTGTGC CCTAGTGCAC 
ATTCTTCAAA GTACTGGCAG AGCCAAGGAT TTTCTGACCG ACTTGGTGAT GTCTGAGGTG 
GATCGCTGTG GAGAGCATGA CGTCTTGATC TTCAGAGAGA ACACCATTGC TACCAAATCC 
ATTGAGGAAT ACCTCAAGTT GGTGGGACAA CAGTATCTTC ATGACGCATT GGGGGAGTTC 
ATCAAAGCTT TGTACGAGTC AGATGAGAAC TGTGAAGTGG ATCCCAGCAA GTGTTCCTCT 
AGTGAACTGA TAGACCATCA GAGCAACCTG AAGATGTGCT GTGAGCTGGC TTTCTGCAAG 
ATCATCAACT CTTACTGTGT TTTCCCTCGT GAGTTGAAAG AAGTGTTTGC ATCGTGGAAG 
CAGCAGTGCC TGAACCGTGG CAAACAGGAC ATCAGCGAGC GGCTCATCAG TGCCTCATTG 
TTTCTCCGTT TCCTGTGCCC TGCCATCATG TCTCCCAGTC TCTTCAACCT TATGCAAGAG 
TACCCTGATG ACCGTACATC TCGCACCCTG ACTCTGATTG CCAAGGTCAT TCAGAACCTG 
GCTAACTTTG CCAAGTTTGG TAACAAAGAA GAATATATGG CATTCATGAA TGATTTTTTA 
GAACATGAAT GGGGTGGAAT GAAGCGCTTT CTTTTGGAAA TCTCTAATCC GGACACCATC 
TCAAACACCC CAGGCTTTGA TGGTTACATT GATCTGGGCC GAGAGCTTTC AGTCTTGCAT 
TCCTTACTAT GGGAAGTAGT TTCCCAGCTT GATAAGGGTG AAAATTCCTT CCTACAGGCG 
ACCGTGGCAA AATTGGGACC GCTCCCTCGA GTTCTTGCTG ATATTACCAA GTCTCTAACT 
AATCCTACAC CAATACAACA GCAGCTGAGA CGCTTCACTG AGCATAACTC CAGTCCAAAT 
GTCAGTGGAA GCCTCTCCTC TGGGCTGCAG AAAATATTTG AAGACCCCAC TGACAGTGAT 
TTGCATAAAC TAAAATCTCC AAGCCAGGAC AACACAGATG GCTATTTCAG AGGGAAAACG 
TTATTGCTGG TTCAGCAGGC CTCCTCCCAG AGCATGACCT ATTCTGAAAA GGATGAAAAG 
GAAAGTAGCC TTCCTAACGG TCGGAGCATC TCCCTCATGG ACCTTCAAGA CACTCACGCT 
GCTCAAGTAG AGCATGCATC TATCATGCTC GATGTGCCTA TGCGCTTAAC TGGAAGCCAG 
CTTTCCATAA CCCAGGTGGC CAGTATCAAA CAACTGCGGG AAACCCAGAG CACTCCCCAG 
AGTGCACCCC AAGTGCGAAG GCCCTTGCAC CCAGCCTTGA ACCAGCCAGG CAGCCTCCAG 
CCCCTGTCAT TCCAGAACCC TGTCTATCAC CTCAATAACC CAATCCCAGC AATGCCAAAG 
GCCTCTGTGG ATTCTAGTTT GGAGAATCTA AGCACTGCCA GTTCCAGAAG CCAAAGTAAC 
AGTGAAGATT TCAAACTCAG TGGGCCCAGC AATAGCAGCA TGGAAGATTT CACTAAACGT 
AGCACGCAGA GCGAGGATTT CTCCAGGCGG CACACTGTAC CAGACAGACA CATACCTCTT 
GCTCTGCCGC GGCAGAATAG TACCGGGCAG GCCCAGATCC GGAAAATGGA CCAGGCTGGG 
TTAGGTGCCC GAGCCAAAGC CCCGCCGTCC TTGCCACACA GTGCTTCCTT ACGTAGCACG 
GGGAGCATGT CGGTGGCCTC TGCGGCCCTG GTGGCTGAAC CTGTGCAGAA TGGGAGCCGG 
TCCCGGCAGC AGTCCTCTTC CTCCAGAGAG AGCCCCGTTC CCAAAGTGAG AGCGATCCAG 
AGACAACAGA CACAGCAGGT GCAGTCACCT GTGGACTCTG CCACCATGTC CCCAGTAGAG 
AGGACAGCAG CCTGGGTGTT GAACAATGGA CAGTATGAAG AGGATGTGGA AGAGACTGAG 
CAAAATCCAG ATGAATCCAA GCATGCTGAG AAGTATGAGC AGGAAATTAC TAAACTGAAG 
GAGCGCCTGA GAGTGTCCAG CCGGCGCCTT GAGGAATATG AGCGCCGCTT GCTGGTGCAG 
GAGCAGCAGA TGCAGAAGCT GCTGCTGGAA TACAAGGCCA GGCTGGAGGA CAGCGAGGAG 
CGCCTGCGCA GGCAGCAGGA GGAGAAGGAC AGCCAGATGA AAAGCATCAT CAGCAGGCTG 
ATGGCTGTGG AAGAGGAACT GAAGAAGGAT CATGCTGAGA TGCAAGCAGT TATTGATGCA 
AAGCAAAAAA TAATCGATGC ACAGGTCATA AATGGAGATG AGATTATTCA AACAGGAAAA 
ACGAATCGTG TCCCTGGATT CGGCCAACAC CAGACTGATG AGCGCACTGA CCCAAGTGAA 
GGAGCGGTAC AGCATGCAGG TCCGCAATGG CATCTCCCCC ACCAACCCCA CCAAGCTTTC 
CATCACGGAG AATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013970633.1
CDS	258..3695
Translation	MGSSCDKEVPAERSPRRRSISGTSTSEKPNSMDTVNTSPFKVPGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNADDRTRGLPKLKESRSHESLLSPCSAVECLDLGRGEPVSVKPLHSSILGQDFCFEVTYLSGSKCFSCNSASERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYSLPPLHSITVHIYKDVEKKKKKDKNNYVGLVNIPTASVTGRQFVEKWYPVSTPTPNKGKTGGPSIRIKSRFQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEELACALVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTIATKSIEEYLKLVGQQYLHDALGEFIKALYESDENCEVDPSKCSSSELIDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCLNRGKQDISERLISASLFLRFLCPAIMSPSLFNLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNTPGFDGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVAKLGPLPRVLADITKSLTNPTPIQQQLRRFTEHNSSPNVSGSLSSGLQKIFEDPTDSDLHKLKSPSQDNTDGYFRGKTLLLVQQASSQSMTYSEKDEKESSLPNGRSISLMDLQDTHAAQVEHASIMLDVPMRLTGSQLSITQVASIKQLRETQSTPQSAPQVRRPLHPALNQPGSLQPLSFQNPVYHLNNPIPAMPKASVDSSLENLSTASSRSQSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTVPDRHIPLALPRQNSTGQAQIRKMDQAGLGARAKAPPSLPHSASLRSTGSMSVASAALVAEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEDVEETEQNPDESKHAEKYEQEITKLKERLRVSSRRLEEYERRLLVQEQQMQKLLLEYKARLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQVINGDEIIQTGKTNRVPGFGQHQTDERTDPSEGAVQHAGPQWHLPHQPHQAFHHGEW

Target ORF information:

RefSeq Version	XM_014115158.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014115158.2

ORF Insert Sequence:

ATGGGAAGCA GTTGTGATAA AGAGGTGCCG GCAGAAAGGT CCCCACGCAG ACGGAGTATC 
TCAGGGACCA GTACATCAGA GAAACCCAAC TCCATGGACA CTGTAAATAC CTCACCCTTC 
AAAGTACCAG GGTTCTTCAG CAAGCGCCTG AAAGGCTCCA TCAAGAGGAC CAAAAGCCAA 
TCAAAGCTTG ACAGAAACAC GAGCTTTCGG CTACCATCCC TTCGTAATGC AGATGACAGG 
ACTCGCGGTC TGCCTAAACT AAAAGAGTCA CGTTCCCATG AGTCATTGCT GAGCCCTTGT 
AGTGCAGTGG AATGTCTGGA TCTTGGCAGA GGAGAGCCTG TGTCAGTGAA GCCACTCCAT 
AGCAGCATCC TTGGACAAGA CTTCTGCTTT GAGGTTACGT ACTTAAGTGG AAGTAAATGC 
TTCAGCTGTA ACTCTGCTTC AGAGAGAGAT AAGTGGATGG AGAACCTTCG TAGGACAGTT 
CAACCAAATA AGGACAATTG CAGACGAGCT GAAAACGTTC TTCGTTTATG GATCATTGAA 
GCCAAGGACC TTGCCCCCAA AAAGAAATAT TTCTGTGAAC TGTGCCTTGA TGATACCCTC 
TTTGCTCGTA CAACCAGCAA GACCAAAGCA GATAATATTT TCTGGGGTGA ACATTTTGAA 
TTTTACAGCC TTCCACCTCT TCATAGCATC ACAGTTCACA TTTATAAAGA TGTGGAAAAA 
AAGAAAAAAA AAGACAAGAA TAATTATGTA GGGCTAGTCA ACATTCCCAC TGCCAGTGTG 
ACTGGGCGCC AATTTGTAGA AAAGTGGTAT CCAGTGAGTA CCCCTACACC CAACAAAGGA 
AAGACCGGAG GACCTTCTAT TCGGATTAAA TCACGTTTCC AAACTATCAC CATTCTGCCT 
ATGGAGCAAT ACAAAGAATT TGCAGAATTT GTCACCAGCA ACTATACCAT GCTGTGTTCT 
GTCCTTGAAC CAGTAATTAG TGTGAGAAAT AAAGAGGAGT TGGCCTGTGC CCTAGTGCAC 
ATTCTTCAAA GTACTGGCAG AGCCAAGGAT TTTCTGACCG ACTTGGTGAT GTCTGAGGTG 
GATCGCTGTG GAGAGCATGA CGTCTTGATC TTCAGAGAGA ACACCATTGC TACCAAATCC 
ATTGAGGAAT ACCTCAAGTT GGTGGGACAA CAGTATCTTC ATGACGCATT GGGGGAGTTC 
ATCAAAGCTT TGTACGAGTC AGATGAGAAC TGTGAAGTGG ATCCCAGCAA GTGTTCCTCT 
AGTGAACTGA TAGACCATCA GAGCAACCTG AAGATGTGCT GTGAGCTGGC TTTCTGCAAG 
ATCATCAACT CTTACTGTGT TTTCCCTCGT GAGTTGAAAG AAGTGTTTGC ATCGTGGAAG 
CAGCAGTGCC TGAACCGTGG CAAACAGGAC ATCAGCGAGC GGCTCATCAG TGCCTCATTG 
TTTCTCCGTT TCCTGTGCCC TGCCATCATG TCTCCCAGTC TCTTCAACCT TATGCAAGAG 
TACCCTGATG ACCGTACATC TCGCACCCTG ACTCTGATTG CCAAGGTCAT TCAGAACCTG 
GCTAACTTTG CCAAGTTTGG TAACAAAGAA GAATATATGG CATTCATGAA TGATTTTTTA 
GAACATGAAT GGGGTGGAAT GAAGCGCTTT CTTTTGGAAA TCTCTAATCC GGACACCATC 
TCAAACACCC CAGGCTTTGA TGGTTACATT GATCTGGGCC GAGAGCTTTC AGTCTTGCAT 
TCCTTACTAT GGGAAGTAGT TTCCCAGCTT GATAAGGGTG AAAATTCCTT CCTACAGGCG 
ACCGTGGCAA AATTGGGACC GCTCCCTCGA GTTCTTGCTG ATATTACCAA GTCTCTAACT 
AATCCTACAC CAATACAACA GCAGCTGAGA CGCTTCACTG AGCATAACTC CAGTCCAAAT 
GTCAGTGGAA GCCTCTCCTC TGGGCTGCAG AAAATATTTG AAGACCCCAC TGACAGTGAT 
TTGCATAAAC TAAAATCTCC AAGCCAGGAC AACACAGATG GCTATTTCAG AGGGAAAACG 
TTATTGCTGG TTCAGCAGGC CTCCTCCCAG AGCATGACCT ATTCTGAAAA GGATGAAAAG 
GAAAGTAGCC TTCCTAACGG TCGGAGCATC TCCCTCATGG ACCTTCAAGA CACTCACGCT 
GCTCAAGTAG AGCATGCATC TATCATGCTC GATGTGCCTA TGCGCTTAAC TGGAAGCCAG 
CTTTCCATAA CCCAGGTGGC CAGTATCAAA CAACTGCGGG AAACCCAGAG CACTCCCCAG 
AGTGCACCCC AAGTGCGAAG GCCCTTGCAC CCAGCCTTGA ACCAGCCAGG CAGCCTCCAG 
CCCCTGTCAT TCCAGAACCC TGTCTATCAC CTCAATAACC CAATCCCAGC AATGCCAAAG 
GCCTCTGTGG ATTCTAGTTT GGAGAATCTA AGCACTGCCA GTTCCAGAAG CCAAAGTAAC 
AGTGAAGATT TCAAACTCAG TGGGCCCAGC AATAGCAGCA TGGAAGATTT CACTAAACGT 
AGCACGCAGA GCGAGGATTT CTCCAGGCGG CACACTGTAC CAGACAGACA CATACCTCTT 
GCTCTGCCGC GGCAGAATAG TACCGGGCAG GCCCAGATCC GGAAAATGGA CCAGGCTGGG 
TTAGGTGCCC GAGCCAAAGC CCCGCCGTCC TTGCCACACA GTGCTTCCTT ACGTAGCACG 
GGGAGCATGT CGGTGGCCTC TGCGGCCCTG GTGGCTGAAC CTGTGCAGAA TGGGAGCCGG 
TCCCGGCAGC AGTCCTCTTC CTCCAGAGAG AGCCCCGTTC CCAAAGTGAG AGCGATCCAG 
AGACAACAGA CACAGCAGGT GCAGTCACCT GTGGACTCTG CCACCATGTC CCCAGTAGAG 
AGGACAGCAG CCTGGGTGTT GAACAATGGA CAGTATGAAG AGGATGTGGA AGAGACTGAG 
CAAAATCCAG ATGAATCCAA GCATGCTGAG AAGTATGAGC AGGAAATTAC TAAACTGAAG 
GAGCGCCTGA GAGTGTCCAG CCGGCGCCTT GAGGAATATG AGCGCCGCTT GCTGGTGCAG 
GAGCAGCAGA TGCAGAAGCT GCTGCTGGAA TACAAGGCCA GGCTGGAGGA CAGCGAGGAG 
CGCCTGCGCA GGCAGCAGGA GGAGAAGGAC AGCCAGATGA AAAGCATCAT CAGCAGGCTG 
ATGGCTGTGG AAGAGGAACT GAAGAAGGAT CATGCTGAGA TGCAAGCAGT TATTGATGCA 
AAGCAAAAAA TAATCGATGC ACAGGTCATA AATGGAGATG AGATTATTCA AACAGGAAAA 
ACGAATCGTG TCCCTGGATT CGGCCAACAC CAGACTGATG AGCGCACTGA CCCAAGTGAA 
GGAGCGGTAC AGCATGCAGG TCCGCAATGG CATCTCCCCC ACCAACCCCA CCAAGCTTTC 
CATCACGGAG AATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_014115159.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013970634.1 Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X12, mRNA.

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CloneID	OCe37441
Clone ID Related Accession (Same CDS sequence)	XM_014115159.2 , XM_014115159.1
Accession Version	XM_014115159.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3429bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	ras GTPase-activating protein nGAP isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014115159.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGACCC CAGAGGTGCC GGCAGAAAGG TCCCCACGCA GACGGAGTAT CTCAGGGACC 
AGTACATCAG AGAAACCCAA CTCCATGGAC ACTGTAAATA CCTCACCCTT CAAAGTACCA 
GGGTTCTTCA GCAAGCGCCT GAAAGGCTCC ATCAAGAGGA CCAAAAGCCA ATCAAAGCTT 
GACAGAAACA CGAGCTTTCG GCTACCATCC CTTCGTAATG CAGATGACAG GACTCGCGGT 
CTGCCTAAAC TAAAAGAGTC ACGTTCCCAT GAGTCATTGC TGAGCCCTTG TAGTGCAGTG 
GAATGTCTGG ATCTTGGCAG AGGAGAGCCT GTGTCAGTGA AGCCACTCCA TAGCAGCATC 
CTTGGACAAG ACTTCTGCTT TGAGGTTACG TACTTAAGTG GAAGTAAATG CTTCAGCTGT 
AACTCTGCTT CAGAGAGAGA TAAGTGGATG GAGAACCTTC GTAGGACAGT TCAACCAAAT 
AAGGACAATT GCAGACGAGC TGAAAACGTT CTTCGTTTAT GGATCATTGA AGCCAAGGAC 
CTTGCCCCCA AAAAGAAATA TTTCTGTGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT 
ACAACCAGCA AGACCAAAGC AGATAATATT TTCTGGGGTG AACATTTTGA ATTTTACAGC 
CTTCCACCTC TTCATAGCAT CACAGTTCAC ATTTATAAAG ATGTGGAAAA AAAGAAAAAA 
AAAGACAAGA ATAATTATGT AGGGCTAGTC AACATTCCCA CTGCCAGTGT GACTGGGCGC 
CAATTTGTAG AAAAGTGGTA TCCAGTGAGT ACCCCTACAC CCAACAAAGG AAAGACCGGA 
GGACCTTCTA TTCGGATTAA ATCACGTTTC CAAACTATCA CCATTCTGCC TATGGAGCAA 
TACAAAGAAT TTGCAGAATT TGTCACCAGC AACTATACCA TGCTGTGTTC TGTCCTTGAA 
CCAGTAATTA GTGTGAGAAA TAAAGAGGAG TTGGCCTGTG CCCTAGTGCA CATTCTTCAA 
AGTACTGGCA GAGCCAAGGA TTTTCTGACC GACTTGGTGA TGTCTGAGGT GGATCGCTGT 
GGAGAGCATG ACGTCTTGAT CTTCAGAGAG AACACCATTG CTACCAAATC CATTGAGGAA 
TACCTCAAGT TGGTGGGACA ACAGTATCTT CATGACGCAT TGGGGGAGTT CATCAAAGCT 
TTGTACGAGT CAGATGAGAA CTGTGAAGTG GATCCCAGCA AGTGTTCCTC TAGTGAACTG 
ATAGACCATC AGAGCAACCT GAAGATGTGC TGTGAGCTGG CTTTCTGCAA GATCATCAAC 
TCTTACTGTG TTTTCCCTCG TGAGTTGAAA GAAGTGTTTG CATCGTGGAA GCAGCAGTGC 
CTGAACCGTG GCAAACAGGA CATCAGCGAG CGGCTCATCA GTGCCTCATT GTTTCTCCGT 
TTCCTGTGCC CTGCCATCAT GTCTCCCAGT CTCTTCAACC TTATGCAAGA GTACCCTGAT 
GACCGTACAT CTCGCACCCT GACTCTGATT GCCAAGGTCA TTCAGAACCT GGCTAACTTT 
GCCAAGTTTG GTAACAAAGA AGAATATATG GCATTCATGA ATGATTTTTT AGAACATGAA 
TGGGGTGGAA TGAAGCGCTT TCTTTTGGAA ATCTCTAATC CGGACACCAT CTCAAACACC 
CCAGGCTTTG ATGGTTACAT TGATCTGGGC CGAGAGCTTT CAGTCTTGCA TTCCTTACTA 
TGGGAAGTAG TTTCCCAGCT TGATAAGGGT GAAAATTCCT TCCTACAGGC GACCGTGGCA 
AAATTGGGAC CGCTCCCTCG AGTTCTTGCT GATATTACCA AGTCTCTAAC TAATCCTACA 
CCAATACAAC AGCAGCTGAG ACGCTTCACT GAGCATAACT CCAGTCCAAA TGTCAGTGGA 
AGCCTCTCCT CTGGGCTGCA GAAAATATTT GAAGACCCCA CTGACAGTGA TTTGCATAAA 
CTAAAATCTC CAAGCCAGGA CAACACAGAT GGCTATTTCA GAGGGAAAAC GTTATTGCTG 
GTTCAGCAGG CCTCCTCCCA GAGCATGACC TATTCTGAAA AGGATGAAAA GGAAAGTAGC 
CTTCCTAACG GTCGGAGCAT CTCCCTCATG GACCTTCAAG ACACTCACGC TGCTCAAGTA 
GAGCATGCAT CTATCATGCT CGATGTGCCT ATGCGCTTAA CTGGAAGCCA GCTTTCCATA 
ACCCAGGTGG CCAGTATCAA ACAACTGCGG GAAACCCAGA GCACTCCCCA GAGTGCACCC 
CAAGTGCGAA GGCCCTTGCA CCCAGCCTTG AACCAGCCAG GCAGCCTCCA GCCCCTGTCA 
TTCCAGAACC CTGTCTATCA CCTCAATAAC CCAATCCCAG CAATGCCAAA GGCCTCTGTG 
GATTCTAGTT TGGAGAATCT AAGCACTGCC AGTTCCAGAA GCCAAAGTAA CAGTGAAGAT 
TTCAAACTCA GTGGGCCCAG CAATAGCAGC ATGGAAGATT TCACTAAACG TAGCACGCAG 
AGCGAGGATT TCTCCAGGCG GCACACTGTA CCAGACAGAC ACATACCTCT TGCTCTGCCG 
CGGCAGAATA GTACCGGGCA GGCCCAGATC CGGAAAATGG ACCAGGCTGG GTTAGGTGCC 
CGAGCCAAAG CCCCGCCGTC CTTGCCACAC AGTGCTTCCT TACGTAGCAC GGGGAGCATG 
TCGGTGGCCT CTGCGGCCCT GGTGGCTGAA CCTGTGCAGA ATGGGAGCCG GTCCCGGCAG 
CAGTCCTCTT CCTCCAGAGA GAGCCCCGTT CCCAAAGTGA GAGCGATCCA GAGACAACAG 
ACACAGCAGG TGCAGTCACC TGTGGACTCT GCCACCATGT CCCCAGTAGA GAGGACAGCA 
GCCTGGGTGT TGAACAATGG ACAGTATGAA GAGGATGTGG AAGAGACTGA GCAAAATCCA 
GATGAATCCA AGCATGCTGA GAAGTATGAG CAGGAAATTA CTAAACTGAA GGAGCGCCTG 
AGAGTGTCCA GCCGGCGCCT TGAGGAATAT GAGCGCCGCT TGCTGGTGCA GGAGCAGCAG 
ATGCAGAAGC TGCTGCTGGA ATACAAGGCC AGGCTGGAGG ACAGCGAGGA GCGCCTGCGC 
AGGCAGCAGG AGGAGAAGGA CAGCCAGATG AAAAGCATCA TCAGCAGGCT GATGGCTGTG 
GAAGAGGAAC TGAAGAAGGA TCATGCTGAG ATGCAAGCAG TTATTGATGC AAAGCAAAAA 
ATAATCGATG CACAGGTCAT AAATGGAGAT GAGATTATTC AAACAGGAAA AACGAATCGT 
GTCCCTGGAT TCGGCCAACA CCAGACTGAT GAGCGCACTG ACCCAAGTGA AGGAGCGGTA 
CAGCATGCAG GTCCGCAATG GCATCTCCCC CACCAACCCC ACCAAGCTTT CCATCACGGA 
GAATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013970634.1
CDS	137..3565
Translation	MQTPEVPAERSPRRRSISGTSTSEKPNSMDTVNTSPFKVPGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNADDRTRGLPKLKESRSHESLLSPCSAVECLDLGRGEPVSVKPLHSSILGQDFCFEVTYLSGSKCFSCNSASERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYSLPPLHSITVHIYKDVEKKKKKDKNNYVGLVNIPTASVTGRQFVEKWYPVSTPTPNKGKTGGPSIRIKSRFQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEELACALVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTIATKSIEEYLKLVGQQYLHDALGEFIKALYESDENCEVDPSKCSSSELIDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCLNRGKQDISERLISASLFLRFLCPAIMSPSLFNLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNTPGFDGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVAKLGPLPRVLADITKSLTNPTPIQQQLRRFTEHNSSPNVSGSLSSGLQKIFEDPTDSDLHKLKSPSQDNTDGYFRGKTLLLVQQASSQSMTYSEKDEKESSLPNGRSISLMDLQDTHAAQVEHASIMLDVPMRLTGSQLSITQVASIKQLRETQSTPQSAPQVRRPLHPALNQPGSLQPLSFQNPVYHLNNPIPAMPKASVDSSLENLSTASSRSQSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTVPDRHIPLALPRQNSTGQAQIRKMDQAGLGARAKAPPSLPHSASLRSTGSMSVASAALVAEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEDVEETEQNPDESKHAEKYEQEITKLKERLRVSSRRLEEYERRLLVQEQQMQKLLLEYKARLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQVINGDEIIQTGKTNRVPGFGQHQTDERTDPSEGAVQHAGPQWHLPHQPHQAFHHGEW

Target ORF information:

RefSeq Version	XM_014115159.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014115159.2

ORF Insert Sequence:

ATGCAGACCC CAGAGGTGCC GGCAGAAAGG TCCCCACGCA GACGGAGTAT CTCAGGGACC 
AGTACATCAG AGAAACCCAA CTCCATGGAC ACTGTAAATA CCTCACCCTT CAAAGTACCA 
GGGTTCTTCA GCAAGCGCCT GAAAGGCTCC ATCAAGAGGA CCAAAAGCCA ATCAAAGCTT 
GACAGAAACA CGAGCTTTCG GCTACCATCC CTTCGTAATG CAGATGACAG GACTCGCGGT 
CTGCCTAAAC TAAAAGAGTC ACGTTCCCAT GAGTCATTGC TGAGCCCTTG TAGTGCAGTG 
GAATGTCTGG ATCTTGGCAG AGGAGAGCCT GTGTCAGTGA AGCCACTCCA TAGCAGCATC 
CTTGGACAAG ACTTCTGCTT TGAGGTTACG TACTTAAGTG GAAGTAAATG CTTCAGCTGT 
AACTCTGCTT CAGAGAGAGA TAAGTGGATG GAGAACCTTC GTAGGACAGT TCAACCAAAT 
AAGGACAATT GCAGACGAGC TGAAAACGTT CTTCGTTTAT GGATCATTGA AGCCAAGGAC 
CTTGCCCCCA AAAAGAAATA TTTCTGTGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT 
ACAACCAGCA AGACCAAAGC AGATAATATT TTCTGGGGTG AACATTTTGA ATTTTACAGC 
CTTCCACCTC TTCATAGCAT CACAGTTCAC ATTTATAAAG ATGTGGAAAA AAAGAAAAAA 
AAAGACAAGA ATAATTATGT AGGGCTAGTC AACATTCCCA CTGCCAGTGT GACTGGGCGC 
CAATTTGTAG AAAAGTGGTA TCCAGTGAGT ACCCCTACAC CCAACAAAGG AAAGACCGGA 
GGACCTTCTA TTCGGATTAA ATCACGTTTC CAAACTATCA CCATTCTGCC TATGGAGCAA 
TACAAAGAAT TTGCAGAATT TGTCACCAGC AACTATACCA TGCTGTGTTC TGTCCTTGAA 
CCAGTAATTA GTGTGAGAAA TAAAGAGGAG TTGGCCTGTG CCCTAGTGCA CATTCTTCAA 
AGTACTGGCA GAGCCAAGGA TTTTCTGACC GACTTGGTGA TGTCTGAGGT GGATCGCTGT 
GGAGAGCATG ACGTCTTGAT CTTCAGAGAG AACACCATTG CTACCAAATC CATTGAGGAA 
TACCTCAAGT TGGTGGGACA ACAGTATCTT CATGACGCAT TGGGGGAGTT CATCAAAGCT 
TTGTACGAGT CAGATGAGAA CTGTGAAGTG GATCCCAGCA AGTGTTCCTC TAGTGAACTG 
ATAGACCATC AGAGCAACCT GAAGATGTGC TGTGAGCTGG CTTTCTGCAA GATCATCAAC 
TCTTACTGTG TTTTCCCTCG TGAGTTGAAA GAAGTGTTTG CATCGTGGAA GCAGCAGTGC 
CTGAACCGTG GCAAACAGGA CATCAGCGAG CGGCTCATCA GTGCCTCATT GTTTCTCCGT 
TTCCTGTGCC CTGCCATCAT GTCTCCCAGT CTCTTCAACC TTATGCAAGA GTACCCTGAT 
GACCGTACAT CTCGCACCCT GACTCTGATT GCCAAGGTCA TTCAGAACCT GGCTAACTTT 
GCCAAGTTTG GTAACAAAGA AGAATATATG GCATTCATGA ATGATTTTTT AGAACATGAA 
TGGGGTGGAA TGAAGCGCTT TCTTTTGGAA ATCTCTAATC CGGACACCAT CTCAAACACC 
CCAGGCTTTG ATGGTTACAT TGATCTGGGC CGAGAGCTTT CAGTCTTGCA TTCCTTACTA 
TGGGAAGTAG TTTCCCAGCT TGATAAGGGT GAAAATTCCT TCCTACAGGC GACCGTGGCA 
AAATTGGGAC CGCTCCCTCG AGTTCTTGCT GATATTACCA AGTCTCTAAC TAATCCTACA 
CCAATACAAC AGCAGCTGAG ACGCTTCACT GAGCATAACT CCAGTCCAAA TGTCAGTGGA 
AGCCTCTCCT CTGGGCTGCA GAAAATATTT GAAGACCCCA CTGACAGTGA TTTGCATAAA 
CTAAAATCTC CAAGCCAGGA CAACACAGAT GGCTATTTCA GAGGGAAAAC GTTATTGCTG 
GTTCAGCAGG CCTCCTCCCA GAGCATGACC TATTCTGAAA AGGATGAAAA GGAAAGTAGC 
CTTCCTAACG GTCGGAGCAT CTCCCTCATG GACCTTCAAG ACACTCACGC TGCTCAAGTA 
GAGCATGCAT CTATCATGCT CGATGTGCCT ATGCGCTTAA CTGGAAGCCA GCTTTCCATA 
ACCCAGGTGG CCAGTATCAA ACAACTGCGG GAAACCCAGA GCACTCCCCA GAGTGCACCC 
CAAGTGCGAA GGCCCTTGCA CCCAGCCTTG AACCAGCCAG GCAGCCTCCA GCCCCTGTCA 
TTCCAGAACC CTGTCTATCA CCTCAATAAC CCAATCCCAG CAATGCCAAA GGCCTCTGTG 
GATTCTAGTT TGGAGAATCT AAGCACTGCC AGTTCCAGAA GCCAAAGTAA CAGTGAAGAT 
TTCAAACTCA GTGGGCCCAG CAATAGCAGC ATGGAAGATT TCACTAAACG TAGCACGCAG 
AGCGAGGATT TCTCCAGGCG GCACACTGTA CCAGACAGAC ACATACCTCT TGCTCTGCCG 
CGGCAGAATA GTACCGGGCA GGCCCAGATC CGGAAAATGG ACCAGGCTGG GTTAGGTGCC 
CGAGCCAAAG CCCCGCCGTC CTTGCCACAC AGTGCTTCCT TACGTAGCAC GGGGAGCATG 
TCGGTGGCCT CTGCGGCCCT GGTGGCTGAA CCTGTGCAGA ATGGGAGCCG GTCCCGGCAG 
CAGTCCTCTT CCTCCAGAGA GAGCCCCGTT CCCAAAGTGA GAGCGATCCA GAGACAACAG 
ACACAGCAGG TGCAGTCACC TGTGGACTCT GCCACCATGT CCCCAGTAGA GAGGACAGCA 
GCCTGGGTGT TGAACAATGG ACAGTATGAA GAGGATGTGG AAGAGACTGA GCAAAATCCA 
GATGAATCCA AGCATGCTGA GAAGTATGAG CAGGAAATTA CTAAACTGAA GGAGCGCCTG 
AGAGTGTCCA GCCGGCGCCT TGAGGAATAT GAGCGCCGCT TGCTGGTGCA GGAGCAGCAG 
ATGCAGAAGC TGCTGCTGGA ATACAAGGCC AGGCTGGAGG ACAGCGAGGA GCGCCTGCGC 
AGGCAGCAGG AGGAGAAGGA CAGCCAGATG AAAAGCATCA TCAGCAGGCT GATGGCTGTG 
GAAGAGGAAC TGAAGAAGGA TCATGCTGAG ATGCAAGCAG TTATTGATGC AAAGCAAAAA 
ATAATCGATG CACAGGTCAT AAATGGAGAT GAGATTATTC AAACAGGAAA AACGAATCGT 
GTCCCTGGAT TCGGCCAACA CCAGACTGAT GAGCGCACTG ACCCAAGTGA AGGAGCGGTA 
CAGCATGCAG GTCCGCAATG GCATCTCCCC CACCAACCCC ACCAAGCTTT CCATCACGGA 
GAATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_014115159.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013970634.1 Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OCe37441
Clone ID Related Accession (Same CDS sequence)	XM_014115159.2 , XM_014115159.1
Accession Version	XM_014115159.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3429bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	ras GTPase-activating protein nGAP isoform X10
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGACCC CAGAGGTGCC GGCAGAAAGG TCCCCACGCA GACGGAGTAT CTCAGGGACC 
AGTACATCAG AGAAACCCAA CTCCATGGAC ACTGTAAATA CCTCACCCTT CAAAGTACCA 
GGGTTCTTCA GCAAGCGCCT GAAAGGCTCC ATCAAGAGGA CCAAAAGCCA ATCAAAGCTT 
GACAGAAACA CGAGCTTTCG GCTACCATCC CTTCGTAATG CAGATGACAG GACTCGCGGT 
CTGCCTAAAC TAAAAGAGTC ACGTTCCCAT GAGTCATTGC TGAGCCCTTG TAGTGCAGTG 
GAATGTCTGG ATCTTGGCAG AGGAGAGCCT GTGTCAGTGA AGCCACTCCA TAGCAGCATC 
CTTGGACAAG ACTTCTGCTT TGAGGTTACG TACTTAAGTG GAAGTAAATG CTTCAGCTGT 
AACTCTGCTT CAGAGAGAGA TAAGTGGATG GAGAACCTTC GTAGGACAGT TCAACCAAAT 
AAGGACAATT GCAGACGAGC TGAAAACGTT CTTCGTTTAT GGATCATTGA AGCCAAGGAC 
CTTGCCCCCA AAAAGAAATA TTTCTGTGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT 
ACAACCAGCA AGACCAAAGC AGATAATATT TTCTGGGGTG AACATTTTGA ATTTTACAGC 
CTTCCACCTC TTCATAGCAT CACAGTTCAC ATTTATAAAG ATGTGGAAAA AAAGAAAAAA 
AAAGACAAGA ATAATTATGT AGGGCTAGTC AACATTCCCA CTGCCAGTGT GACTGGGCGC 
CAATTTGTAG AAAAGTGGTA TCCAGTGAGT ACCCCTACAC CCAACAAAGG AAAGACCGGA 
GGACCTTCTA TTCGGATTAA ATCACGTTTC CAAACTATCA CCATTCTGCC TATGGAGCAA 
TACAAAGAAT TTGCAGAATT TGTCACCAGC AACTATACCA TGCTGTGTTC TGTCCTTGAA 
CCAGTAATTA GTGTGAGAAA TAAAGAGGAG TTGGCCTGTG CCCTAGTGCA CATTCTTCAA 
AGTACTGGCA GAGCCAAGGA TTTTCTGACC GACTTGGTGA TGTCTGAGGT GGATCGCTGT 
GGAGAGCATG ACGTCTTGAT CTTCAGAGAG AACACCATTG CTACCAAATC CATTGAGGAA 
TACCTCAAGT TGGTGGGACA ACAGTATCTT CATGACGCAT TGGGGGAGTT CATCAAAGCT 
TTGTACGAGT CAGATGAGAA CTGTGAAGTG GATCCCAGCA AGTGTTCCTC TAGTGAACTG 
ATAGACCATC AGAGCAACCT GAAGATGTGC TGTGAGCTGG CTTTCTGCAA GATCATCAAC 
TCTTACTGTG TTTTCCCTCG TGAGTTGAAA GAAGTGTTTG CATCGTGGAA GCAGCAGTGC 
CTGAACCGTG GCAAACAGGA CATCAGCGAG CGGCTCATCA GTGCCTCATT GTTTCTCCGT 
TTCCTGTGCC CTGCCATCAT GTCTCCCAGT CTCTTCAACC TTATGCAAGA GTACCCTGAT 
GACCGTACAT CTCGCACCCT GACTCTGATT GCCAAGGTCA TTCAGAACCT GGCTAACTTT 
GCCAAGTTTG GTAACAAAGA AGAATATATG GCATTCATGA ATGATTTTTT AGAACATGAA 
TGGGGTGGAA TGAAGCGCTT TCTTTTGGAA ATCTCTAATC CGGACACCAT CTCAAACACC 
CCAGGCTTTG ATGGTTACAT TGATCTGGGC CGAGAGCTTT CAGTCTTGCA TTCCTTACTA 
TGGGAAGTAG TTTCCCAGCT TGATAAGGGT GAAAATTCCT TCCTACAGGC GACCGTGGCA 
AAATTGGGAC CGCTCCCTCG AGTTCTTGCT GATATTACCA AGTCTCTAAC TAATCCTACA 
CCAATACAAC AGCAGCTGAG ACGCTTCACT GAGCATAACT CCAGTCCAAA TGTCAGTGGA 
AGCCTCTCCT CTGGGCTGCA GAAAATATTT GAAGACCCCA CTGACAGTGA TTTGCATAAA 
CTAAAATCTC CAAGCCAGGA CAACACAGAT GGCTATTTCA GAGGGAAAAC GTTATTGCTG 
GTTCAGCAGG CCTCCTCCCA GAGCATGACC TATTCTGAAA AGGATGAAAA GGAAAGTAGC 
CTTCCTAACG GTCGGAGCAT CTCCCTCATG GACCTTCAAG ACACTCACGC TGCTCAAGTA 
GAGCATGCAT CTATCATGCT CGATGTGCCT ATGCGCTTAA CTGGAAGCCA GCTTTCCATA 
ACCCAGGTGG CCAGTATCAA ACAACTGCGG GAAACCCAGA GCACTCCCCA GAGTGCACCC 
CAAGTGCGAA GGCCCTTGCA CCCAGCCTTG AACCAGCCAG GCAGCCTCCA GCCCCTGTCA 
TTCCAGAACC CTGTCTATCA CCTCAATAAC CCAATCCCAG CAATGCCAAA GGCCTCTGTG 
GATTCTAGTT TGGAGAATCT AAGCACTGCC AGTTCCAGAA GCCAAAGTAA CAGTGAAGAT 
TTCAAACTCA GTGGGCCCAG CAATAGCAGC ATGGAAGATT TCACTAAACG TAGCACGCAG 
AGCGAGGATT TCTCCAGGCG GCACACTGTA CCAGACAGAC ACATACCTCT TGCTCTGCCG 
CGGCAGAATA GTACCGGGCA GGCCCAGATC CGGAAAATGG ACCAGGCTGG GTTAGGTGCC 
CGAGCCAAAG CCCCGCCGTC CTTGCCACAC AGTGCTTCCT TACGTAGCAC GGGGAGCATG 
TCGGTGGCCT CTGCGGCCCT GGTGGCTGAA CCTGTGCAGA ATGGGAGCCG GTCCCGGCAG 
CAGTCCTCTT CCTCCAGAGA GAGCCCCGTT CCCAAAGTGA GAGCGATCCA GAGACAACAG 
ACACAGCAGG TGCAGTCACC TGTGGACTCT GCCACCATGT CCCCAGTAGA GAGGACAGCA 
GCCTGGGTGT TGAACAATGG ACAGTATGAA GAGGATGTGG AAGAGACTGA GCAAAATCCA 
GATGAATCCA AGCATGCTGA GAAGTATGAG CAGGAAATTA CTAAACTGAA GGAGCGCCTG 
AGAGTGTCCA GCCGGCGCCT TGAGGAATAT GAGCGCCGCT TGCTGGTGCA GGAGCAGCAG 
ATGCAGAAGC TGCTGCTGGA ATACAAGGCC AGGCTGGAGG ACAGCGAGGA GCGCCTGCGC 
AGGCAGCAGG AGGAGAAGGA CAGCCAGATG AAAAGCATCA TCAGCAGGCT GATGGCTGTG 
GAAGAGGAAC TGAAGAAGGA TCATGCTGAG ATGCAAGCAG TTATTGATGC AAAGCAAAAA 
ATAATCGATG CACAGGTCAT AAATGGAGAT GAGATTATTC AAACAGGAAA AACGAATCGT 
GTCCCTGGAT TCGGCCAACA CCAGACTGAT GAGCGCACTG ACCCAAGTGA AGGAGCGGTA 
CAGCATGCAG GTCCGCAATG GCATCTCCCC CACCAACCCC ACCAAGCTTT CCATCACGGA 
GAATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013970634.1
CDS	236..3664
Translation	MQTPEVPAERSPRRRSISGTSTSEKPNSMDTVNTSPFKVPGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNADDRTRGLPKLKESRSHESLLSPCSAVECLDLGRGEPVSVKPLHSSILGQDFCFEVTYLSGSKCFSCNSASERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYSLPPLHSITVHIYKDVEKKKKKDKNNYVGLVNIPTASVTGRQFVEKWYPVSTPTPNKGKTGGPSIRIKSRFQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEELACALVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTIATKSIEEYLKLVGQQYLHDALGEFIKALYESDENCEVDPSKCSSSELIDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCLNRGKQDISERLISASLFLRFLCPAIMSPSLFNLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNTPGFDGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVAKLGPLPRVLADITKSLTNPTPIQQQLRRFTEHNSSPNVSGSLSSGLQKIFEDPTDSDLHKLKSPSQDNTDGYFRGKTLLLVQQASSQSMTYSEKDEKESSLPNGRSISLMDLQDTHAAQVEHASIMLDVPMRLTGSQLSITQVASIKQLRETQSTPQSAPQVRRPLHPALNQPGSLQPLSFQNPVYHLNNPIPAMPKASVDSSLENLSTASSRSQSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTVPDRHIPLALPRQNSTGQAQIRKMDQAGLGARAKAPPSLPHSASLRSTGSMSVASAALVAEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEDVEETEQNPDESKHAEKYEQEITKLKERLRVSSRRLEEYERRLLVQEQQMQKLLLEYKARLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQVINGDEIIQTGKTNRVPGFGQHQTDERTDPSEGAVQHAGPQWHLPHQPHQAFHHGEW

Target ORF information:

RefSeq Version	XM_014115159.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014115159.1

ORF Insert Sequence:

ATGCAGACCC CAGAGGTGCC GGCAGAAAGG TCCCCACGCA GACGGAGTAT CTCAGGGACC 
AGTACATCAG AGAAACCCAA CTCCATGGAC ACTGTAAATA CCTCACCCTT CAAAGTACCA 
GGGTTCTTCA GCAAGCGCCT GAAAGGCTCC ATCAAGAGGA CCAAAAGCCA ATCAAAGCTT 
GACAGAAACA CGAGCTTTCG GCTACCATCC CTTCGTAATG CAGATGACAG GACTCGCGGT 
CTGCCTAAAC TAAAAGAGTC ACGTTCCCAT GAGTCATTGC TGAGCCCTTG TAGTGCAGTG 
GAATGTCTGG ATCTTGGCAG AGGAGAGCCT GTGTCAGTGA AGCCACTCCA TAGCAGCATC 
CTTGGACAAG ACTTCTGCTT TGAGGTTACG TACTTAAGTG GAAGTAAATG CTTCAGCTGT 
AACTCTGCTT CAGAGAGAGA TAAGTGGATG GAGAACCTTC GTAGGACAGT TCAACCAAAT 
AAGGACAATT GCAGACGAGC TGAAAACGTT CTTCGTTTAT GGATCATTGA AGCCAAGGAC 
CTTGCCCCCA AAAAGAAATA TTTCTGTGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT 
ACAACCAGCA AGACCAAAGC AGATAATATT TTCTGGGGTG AACATTTTGA ATTTTACAGC 
CTTCCACCTC TTCATAGCAT CACAGTTCAC ATTTATAAAG ATGTGGAAAA AAAGAAAAAA 
AAAGACAAGA ATAATTATGT AGGGCTAGTC AACATTCCCA CTGCCAGTGT GACTGGGCGC 
CAATTTGTAG AAAAGTGGTA TCCAGTGAGT ACCCCTACAC CCAACAAAGG AAAGACCGGA 
GGACCTTCTA TTCGGATTAA ATCACGTTTC CAAACTATCA CCATTCTGCC TATGGAGCAA 
TACAAAGAAT TTGCAGAATT TGTCACCAGC AACTATACCA TGCTGTGTTC TGTCCTTGAA 
CCAGTAATTA GTGTGAGAAA TAAAGAGGAG TTGGCCTGTG CCCTAGTGCA CATTCTTCAA 
AGTACTGGCA GAGCCAAGGA TTTTCTGACC GACTTGGTGA TGTCTGAGGT GGATCGCTGT 
GGAGAGCATG ACGTCTTGAT CTTCAGAGAG AACACCATTG CTACCAAATC CATTGAGGAA 
TACCTCAAGT TGGTGGGACA ACAGTATCTT CATGACGCAT TGGGGGAGTT CATCAAAGCT 
TTGTACGAGT CAGATGAGAA CTGTGAAGTG GATCCCAGCA AGTGTTCCTC TAGTGAACTG 
ATAGACCATC AGAGCAACCT GAAGATGTGC TGTGAGCTGG CTTTCTGCAA GATCATCAAC 
TCTTACTGTG TTTTCCCTCG TGAGTTGAAA GAAGTGTTTG CATCGTGGAA GCAGCAGTGC 
CTGAACCGTG GCAAACAGGA CATCAGCGAG CGGCTCATCA GTGCCTCATT GTTTCTCCGT 
TTCCTGTGCC CTGCCATCAT GTCTCCCAGT CTCTTCAACC TTATGCAAGA GTACCCTGAT 
GACCGTACAT CTCGCACCCT GACTCTGATT GCCAAGGTCA TTCAGAACCT GGCTAACTTT 
GCCAAGTTTG GTAACAAAGA AGAATATATG GCATTCATGA ATGATTTTTT AGAACATGAA 
TGGGGTGGAA TGAAGCGCTT TCTTTTGGAA ATCTCTAATC CGGACACCAT CTCAAACACC 
CCAGGCTTTG ATGGTTACAT TGATCTGGGC CGAGAGCTTT CAGTCTTGCA TTCCTTACTA 
TGGGAAGTAG TTTCCCAGCT TGATAAGGGT GAAAATTCCT TCCTACAGGC GACCGTGGCA 
AAATTGGGAC CGCTCCCTCG AGTTCTTGCT GATATTACCA AGTCTCTAAC TAATCCTACA 
CCAATACAAC AGCAGCTGAG ACGCTTCACT GAGCATAACT CCAGTCCAAA TGTCAGTGGA 
AGCCTCTCCT CTGGGCTGCA GAAAATATTT GAAGACCCCA CTGACAGTGA TTTGCATAAA 
CTAAAATCTC CAAGCCAGGA CAACACAGAT GGCTATTTCA GAGGGAAAAC GTTATTGCTG 
GTTCAGCAGG CCTCCTCCCA GAGCATGACC TATTCTGAAA AGGATGAAAA GGAAAGTAGC 
CTTCCTAACG GTCGGAGCAT CTCCCTCATG GACCTTCAAG ACACTCACGC TGCTCAAGTA 
GAGCATGCAT CTATCATGCT CGATGTGCCT ATGCGCTTAA CTGGAAGCCA GCTTTCCATA 
ACCCAGGTGG CCAGTATCAA ACAACTGCGG GAAACCCAGA GCACTCCCCA GAGTGCACCC 
CAAGTGCGAA GGCCCTTGCA CCCAGCCTTG AACCAGCCAG GCAGCCTCCA GCCCCTGTCA 
TTCCAGAACC CTGTCTATCA CCTCAATAAC CCAATCCCAG CAATGCCAAA GGCCTCTGTG 
GATTCTAGTT TGGAGAATCT AAGCACTGCC AGTTCCAGAA GCCAAAGTAA CAGTGAAGAT 
TTCAAACTCA GTGGGCCCAG CAATAGCAGC ATGGAAGATT TCACTAAACG TAGCACGCAG 
AGCGAGGATT TCTCCAGGCG GCACACTGTA CCAGACAGAC ACATACCTCT TGCTCTGCCG 
CGGCAGAATA GTACCGGGCA GGCCCAGATC CGGAAAATGG ACCAGGCTGG GTTAGGTGCC 
CGAGCCAAAG CCCCGCCGTC CTTGCCACAC AGTGCTTCCT TACGTAGCAC GGGGAGCATG 
TCGGTGGCCT CTGCGGCCCT GGTGGCTGAA CCTGTGCAGA ATGGGAGCCG GTCCCGGCAG 
CAGTCCTCTT CCTCCAGAGA GAGCCCCGTT CCCAAAGTGA GAGCGATCCA GAGACAACAG 
ACACAGCAGG TGCAGTCACC TGTGGACTCT GCCACCATGT CCCCAGTAGA GAGGACAGCA 
GCCTGGGTGT TGAACAATGG ACAGTATGAA GAGGATGTGG AAGAGACTGA GCAAAATCCA 
GATGAATCCA AGCATGCTGA GAAGTATGAG CAGGAAATTA CTAAACTGAA GGAGCGCCTG 
AGAGTGTCCA GCCGGCGCCT TGAGGAATAT GAGCGCCGCT TGCTGGTGCA GGAGCAGCAG 
ATGCAGAAGC TGCTGCTGGA ATACAAGGCC AGGCTGGAGG ACAGCGAGGA GCGCCTGCGC 
AGGCAGCAGG AGGAGAAGGA CAGCCAGATG AAAAGCATCA TCAGCAGGCT GATGGCTGTG 
GAAGAGGAAC TGAAGAAGGA TCATGCTGAG ATGCAAGCAG TTATTGATGC AAAGCAAAAA 
ATAATCGATG CACAGGTCAT AAATGGAGAT GAGATTATTC AAACAGGAAA AACGAATCGT 
GTCCCTGGAT TCGGCCAACA CCAGACTGAT GAGCGCACTG ACCCAAGTGA AGGAGCGGTA 
CAGCATGCAG GTCCGCAATG GCATCTCCCC CACCAACCCC ACCAAGCTTT CCATCACGGA 
GAATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_014115160.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013970635.1 Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OCe37442
Clone ID Related Accession (Same CDS sequence)	XM_014115160.1 , XM_014115161.1
Accession Version	XM_014115160.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3345bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	ras GTPase-activating protein nGAP isoform X11
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACTG TAAATACCTC ACCCTTCAAA GTACCAGGGT TCTTCAGCAA GCGCCTGAAA 
GGCTCCATCA AGAGGACCAA AAGCCAATCA AAGCTTGACA GAAACACGAG CTTTCGGCTA 
CCATCCCTTC GTAATGCAGA TGACAGGACT CGCGGTCTGC CTAAACTAAA AGAGTCACGT 
TCCCATGAGT CATTGCTGAG CCCTTGTAGT GCAGTGGAAT GTCTGGATCT TGGCAGAGGA 
GAGCCTGTGT CAGTGAAGCC ACTCCATAGC AGCATCCTTG GACAAGACTT CTGCTTTGAG 
GTTACGTACT TAAGTGGAAG TAAATGCTTC AGCTGTAACT CTGCTTCAGA GAGAGATAAG 
TGGATGGAGA ACCTTCGTAG GACAGTTCAA CCAAATAAGG ACAATTGCAG ACGAGCTGAA 
AACGTTCTTC GTTTATGGAT CATTGAAGCC AAGGACCTTG CCCCCAAAAA GAAATATTTC 
TGTGAACTGT GCCTTGATGA TACCCTCTTT GCTCGTACAA CCAGCAAGAC CAAAGCAGAT 
AATATTTTCT GGGGTGAACA TTTTGAATTT TACAGCCTTC CACCTCTTCA TAGCATCACA 
GTTCACATTT ATAAAGATGT GGAAAAAAAG AAAAAAAAAG ACAAGAATAA TTATGTAGGG 
CTAGTCAACA TTCCCACTGC CAGTGTGACT GGGCGCCAAT TTGTAGAAAA GTGGTATCCA 
GTGAGTACCC CTACACCCAA CAAAGGAAAG ACCGGAGGAC CTTCTATTCG GATTAAATCA 
CGTTTCCAAA CTATCACCAT TCTGCCTATG GAGCAATACA AAGAATTTGC AGAATTTGTC 
ACCAGCAACT ATACCATGCT GTGTTCTGTC CTTGAACCAG TAATTAGTGT GAGAAATAAA 
GAGGAGTTGG CCTGTGCCCT AGTGCACATT CTTCAAAGTA CTGGCAGAGC CAAGGATTTT 
CTGACCGACT TGGTGATGTC TGAGGTGGAT CGCTGTGGAG AGCATGACGT CTTGATCTTC 
AGAGAGAACA CCATTGCTAC CAAATCCATT GAGGAATACC TCAAGTTGGT GGGACAACAG 
TATCTTCATG ACGCATTGGG GGAGTTCATC AAAGCTTTGT ACGAGTCAGA TGAGAACTGT 
GAAGTGGATC CCAGCAAGTG TTCCTCTAGT GAACTGATAG ACCATCAGAG CAACCTGAAG 
ATGTGCTGTG AGCTGGCTTT CTGCAAGATC ATCAACTCTT ACTGTGTTTT CCCTCGTGAG 
TTGAAAGAAG TGTTTGCATC GTGGAAGCAG CAGTGCCTGA ACCGTGGCAA ACAGGACATC 
AGCGAGCGGC TCATCAGTGC CTCATTGTTT CTCCGTTTCC TGTGCCCTGC CATCATGTCT 
CCCAGTCTCT TCAACCTTAT GCAAGAGTAC CCTGATGACC GTACATCTCG CACCCTGACT 
CTGATTGCCA AGGTCATTCA GAACCTGGCT AACTTTGCCA AGTTTGGTAA CAAAGAAGAA 
TATATGGCAT TCATGAATGA TTTTTTAGAA CATGAATGGG GTGGAATGAA GCGCTTTCTT 
TTGGAAATCT CTAATCCGGA CACCATCTCA AACACCCCAG GCTTTGATGG TTACATTGAT 
CTGGGCCGAG AGCTTTCAGT CTTGCATTCC TTACTATGGG AAGTAGTTTC CCAGCTTGAT 
AAGGGTGAAA ATTCCTTCCT ACAGGCGACC GTGGCAAAAT TGGGACCGCT CCCTCGAGTT 
CTTGCTGATA TTACCAAGTC TCTAACTAAT CCTACACCAA TACAACAGCA GCTGAGACGC 
TTCACTGAGC ATAACTCCAG TCCAAATGTC AGTGGAAGCC TCTCCTCTGG GCTGCAGAAA 
ATATTTGAAG ACCCCACTGA CAGTGATTTG CATAAACTAA AATCTCCAAG CCAGGACAAC 
ACAGATGGCT ATTTCAGAGG GAAAACGTTA TTGCTGGTTC AGCAGGCCTC CTCCCAGAGC 
ATGACCTATT CTGAAAAGGA TGAAAAGGAA AGTAGCCTTC CTAACGGTCG GAGCATCTCC 
CTCATGGACC TTCAAGACAC TCACGCTGCT CAAGTAGAGC ATGCATCTAT CATGCTCGAT 
GTGCCTATGC GCTTAACTGG AAGCCAGCTT TCCATAACCC AGGTGGCCAG TATCAAACAA 
CTGCGGGAAA CCCAGAGCAC TCCCCAGAGT GCACCCCAAG TGCGAAGGCC CTTGCACCCA 
GCCTTGAACC AGCCAGGCAG CCTCCAGCCC CTGTCATTCC AGAACCCTGT CTATCACCTC 
AATAACCCAA TCCCAGCAAT GCCAAAGGCC TCTGTGGATT CTAGTTTGGA GAATCTAAGC 
ACTGCCAGTT CCAGAAGCCA AAGTAACAGT GAAGATTTCA AACTCAGTGG GCCCAGCAAT 
AGCAGCATGG AAGATTTCAC TAAACGTAGC ACGCAGAGCG AGGATTTCTC CAGGCGGCAC 
ACTGTACCAG ACAGACACAT ACCTCTTGCT CTGCCGCGGC AGAATAGTAC CGGGCAGGCC 
CAGATCCGGA AAATGGACCA GGCTGGGTTA GGTGCCCGAG CCAAAGCCCC GCCGTCCTTG 
CCACACAGTG CTTCCTTACG TAGCACGGGG AGCATGTCGG TGGCCTCTGC GGCCCTGGTG 
GCTGAACCTG TGCAGAATGG GAGCCGGTCC CGGCAGCAGT CCTCTTCCTC CAGAGAGAGC 
CCCGTTCCCA AAGTGAGAGC GATCCAGAGA CAACAGACAC AGCAGGTGCA GTCACCTGTG 
GACTCTGCCA CCATGTCCCC AGTAGAGAGG ACAGCAGCCT GGGTGTTGAA CAATGGACAG 
TATGAAGAGG ATGTGGAAGA GACTGAGCAA AATCCAGATG AATCCAAGCA TGCTGAGAAG 
TATGAGCAGG AAATTACTAA ACTGAAGGAG CGCCTGAGAG TGTCCAGCCG GCGCCTTGAG 
GAATATGAGC GCCGCTTGCT GGTGCAGGAG CAGCAGATGC AGAAGCTGCT GCTGGAATAC 
AAGGCCAGGC TGGAGGACAG CGAGGAGCGC CTGCGCAGGC AGCAGGAGGA GAAGGACAGC 
CAGATGAAAA GCATCATCAG CAGGCTGATG GCTGTGGAAG AGGAACTGAA GAAGGATCAT 
GCTGAGATGC AAGCAGTTAT TGATGCAAAG CAAAAAATAA TCGATGCACA GGTCATAAAT 
GGAGATGAGA TTATTCAAAC AGGAAAAACG AATCGTGTCC CTGGATTCGG CCAACACCAG 
ACTGATGAGC GCACTGACCC AAGTGAAGGA GCGGTACAGC ATGCAGGTCC GCAATGGCAT 
CTCCCCCACC AACCCCACCA AGCTTTCCAT CACGGAGAAT GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013970635.1
CDS	93..3437
Translation	MDTVNTSPFKVPGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNADDRTRGLPKLKESRSHESLLSPCSAVECLDLGRGEPVSVKPLHSSILGQDFCFEVTYLSGSKCFSCNSASERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYSLPPLHSITVHIYKDVEKKKKKDKNNYVGLVNIPTASVTGRQFVEKWYPVSTPTPNKGKTGGPSIRIKSRFQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEELACALVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTIATKSIEEYLKLVGQQYLHDALGEFIKALYESDENCEVDPSKCSSSELIDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCLNRGKQDISERLISASLFLRFLCPAIMSPSLFNLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNTPGFDGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVAKLGPLPRVLADITKSLTNPTPIQQQLRRFTEHNSSPNVSGSLSSGLQKIFEDPTDSDLHKLKSPSQDNTDGYFRGKTLLLVQQASSQSMTYSEKDEKESSLPNGRSISLMDLQDTHAAQVEHASIMLDVPMRLTGSQLSITQVASIKQLRETQSTPQSAPQVRRPLHPALNQPGSLQPLSFQNPVYHLNNPIPAMPKASVDSSLENLSTASSRSQSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTVPDRHIPLALPRQNSTGQAQIRKMDQAGLGARAKAPPSLPHSASLRSTGSMSVASAALVAEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEDVEETEQNPDESKHAEKYEQEITKLKERLRVSSRRLEEYERRLLVQEQQMQKLLLEYKARLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQVINGDEIIQTGKTNRVPGFGQHQTDERTDPSEGAVQHAGPQWHLPHQPHQAFHHGEW

Target ORF information:

RefSeq Version	XM_014115160.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014115160.1

ORF Insert Sequence:

ATGGACACTG TAAATACCTC ACCCTTCAAA GTACCAGGGT TCTTCAGCAA GCGCCTGAAA 
GGCTCCATCA AGAGGACCAA AAGCCAATCA AAGCTTGACA GAAACACGAG CTTTCGGCTA 
CCATCCCTTC GTAATGCAGA TGACAGGACT CGCGGTCTGC CTAAACTAAA AGAGTCACGT 
TCCCATGAGT CATTGCTGAG CCCTTGTAGT GCAGTGGAAT GTCTGGATCT TGGCAGAGGA 
GAGCCTGTGT CAGTGAAGCC ACTCCATAGC AGCATCCTTG GACAAGACTT CTGCTTTGAG 
GTTACGTACT TAAGTGGAAG TAAATGCTTC AGCTGTAACT CTGCTTCAGA GAGAGATAAG 
TGGATGGAGA ACCTTCGTAG GACAGTTCAA CCAAATAAGG ACAATTGCAG ACGAGCTGAA 
AACGTTCTTC GTTTATGGAT CATTGAAGCC AAGGACCTTG CCCCCAAAAA GAAATATTTC 
TGTGAACTGT GCCTTGATGA TACCCTCTTT GCTCGTACAA CCAGCAAGAC CAAAGCAGAT 
AATATTTTCT GGGGTGAACA TTTTGAATTT TACAGCCTTC CACCTCTTCA TAGCATCACA 
GTTCACATTT ATAAAGATGT GGAAAAAAAG AAAAAAAAAG ACAAGAATAA TTATGTAGGG 
CTAGTCAACA TTCCCACTGC CAGTGTGACT GGGCGCCAAT TTGTAGAAAA GTGGTATCCA 
GTGAGTACCC CTACACCCAA CAAAGGAAAG ACCGGAGGAC CTTCTATTCG GATTAAATCA 
CGTTTCCAAA CTATCACCAT TCTGCCTATG GAGCAATACA AAGAATTTGC AGAATTTGTC 
ACCAGCAACT ATACCATGCT GTGTTCTGTC CTTGAACCAG TAATTAGTGT GAGAAATAAA 
GAGGAGTTGG CCTGTGCCCT AGTGCACATT CTTCAAAGTA CTGGCAGAGC CAAGGATTTT 
CTGACCGACT TGGTGATGTC TGAGGTGGAT CGCTGTGGAG AGCATGACGT CTTGATCTTC 
AGAGAGAACA CCATTGCTAC CAAATCCATT GAGGAATACC TCAAGTTGGT GGGACAACAG 
TATCTTCATG ACGCATTGGG GGAGTTCATC AAAGCTTTGT ACGAGTCAGA TGAGAACTGT 
GAAGTGGATC CCAGCAAGTG TTCCTCTAGT GAACTGATAG ACCATCAGAG CAACCTGAAG 
ATGTGCTGTG AGCTGGCTTT CTGCAAGATC ATCAACTCTT ACTGTGTTTT CCCTCGTGAG 
TTGAAAGAAG TGTTTGCATC GTGGAAGCAG CAGTGCCTGA ACCGTGGCAA ACAGGACATC 
AGCGAGCGGC TCATCAGTGC CTCATTGTTT CTCCGTTTCC TGTGCCCTGC CATCATGTCT 
CCCAGTCTCT TCAACCTTAT GCAAGAGTAC CCTGATGACC GTACATCTCG CACCCTGACT 
CTGATTGCCA AGGTCATTCA GAACCTGGCT AACTTTGCCA AGTTTGGTAA CAAAGAAGAA 
TATATGGCAT TCATGAATGA TTTTTTAGAA CATGAATGGG GTGGAATGAA GCGCTTTCTT 
TTGGAAATCT CTAATCCGGA CACCATCTCA AACACCCCAG GCTTTGATGG TTACATTGAT 
CTGGGCCGAG AGCTTTCAGT CTTGCATTCC TTACTATGGG AAGTAGTTTC CCAGCTTGAT 
AAGGGTGAAA ATTCCTTCCT ACAGGCGACC GTGGCAAAAT TGGGACCGCT CCCTCGAGTT 
CTTGCTGATA TTACCAAGTC TCTAACTAAT CCTACACCAA TACAACAGCA GCTGAGACGC 
TTCACTGAGC ATAACTCCAG TCCAAATGTC AGTGGAAGCC TCTCCTCTGG GCTGCAGAAA 
ATATTTGAAG ACCCCACTGA CAGTGATTTG CATAAACTAA AATCTCCAAG CCAGGACAAC 
ACAGATGGCT ATTTCAGAGG GAAAACGTTA TTGCTGGTTC AGCAGGCCTC CTCCCAGAGC 
ATGACCTATT CTGAAAAGGA TGAAAAGGAA AGTAGCCTTC CTAACGGTCG GAGCATCTCC 
CTCATGGACC TTCAAGACAC TCACGCTGCT CAAGTAGAGC ATGCATCTAT CATGCTCGAT 
GTGCCTATGC GCTTAACTGG AAGCCAGCTT TCCATAACCC AGGTGGCCAG TATCAAACAA 
CTGCGGGAAA CCCAGAGCAC TCCCCAGAGT GCACCCCAAG TGCGAAGGCC CTTGCACCCA 
GCCTTGAACC AGCCAGGCAG CCTCCAGCCC CTGTCATTCC AGAACCCTGT CTATCACCTC 
AATAACCCAA TCCCAGCAAT GCCAAAGGCC TCTGTGGATT CTAGTTTGGA GAATCTAAGC 
ACTGCCAGTT CCAGAAGCCA AAGTAACAGT GAAGATTTCA AACTCAGTGG GCCCAGCAAT 
AGCAGCATGG AAGATTTCAC TAAACGTAGC ACGCAGAGCG AGGATTTCTC CAGGCGGCAC 
ACTGTACCAG ACAGACACAT ACCTCTTGCT CTGCCGCGGC AGAATAGTAC CGGGCAGGCC 
CAGATCCGGA AAATGGACCA GGCTGGGTTA GGTGCCCGAG CCAAAGCCCC GCCGTCCTTG 
CCACACAGTG CTTCCTTACG TAGCACGGGG AGCATGTCGG TGGCCTCTGC GGCCCTGGTG 
GCTGAACCTG TGCAGAATGG GAGCCGGTCC CGGCAGCAGT CCTCTTCCTC CAGAGAGAGC 
CCCGTTCCCA AAGTGAGAGC GATCCAGAGA CAACAGACAC AGCAGGTGCA GTCACCTGTG 
GACTCTGCCA CCATGTCCCC AGTAGAGAGG ACAGCAGCCT GGGTGTTGAA CAATGGACAG 
TATGAAGAGG ATGTGGAAGA GACTGAGCAA AATCCAGATG AATCCAAGCA TGCTGAGAAG 
TATGAGCAGG AAATTACTAA ACTGAAGGAG CGCCTGAGAG TGTCCAGCCG GCGCCTTGAG 
GAATATGAGC GCCGCTTGCT GGTGCAGGAG CAGCAGATGC AGAAGCTGCT GCTGGAATAC 
AAGGCCAGGC TGGAGGACAG CGAGGAGCGC CTGCGCAGGC AGCAGGAGGA GAAGGACAGC 
CAGATGAAAA GCATCATCAG CAGGCTGATG GCTGTGGAAG AGGAACTGAA GAAGGATCAT 
GCTGAGATGC AAGCAGTTAT TGATGCAAAG CAAAAAATAA TCGATGCACA GGTCATAAAT 
GGAGATGAGA TTATTCAAAC AGGAAAAACG AATCGTGTCC CTGGATTCGG CCAACACCAG 
ACTGATGAGC GCACTGACCC AAGTGAAGGA GCGGTACAGC ATGCAGGTCC GCAATGGCAT 
CTCCCCCACC AACCCCACCA AGCTTTCCAT CACGGAGAAT GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_014115161.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013970636.1 Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OCe37442
Clone ID Related Accession (Same CDS sequence)	XM_014115160.1 , XM_014115161.1
Accession Version	XM_014115161.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3345bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	ras GTPase-activating protein nGAP isoform X11
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACTG TAAATACCTC ACCCTTCAAA GTACCAGGGT TCTTCAGCAA GCGCCTGAAA 
GGCTCCATCA AGAGGACCAA AAGCCAATCA AAGCTTGACA GAAACACGAG CTTTCGGCTA 
CCATCCCTTC GTAATGCAGA TGACAGGACT CGCGGTCTGC CTAAACTAAA AGAGTCACGT 
TCCCATGAGT CATTGCTGAG CCCTTGTAGT GCAGTGGAAT GTCTGGATCT TGGCAGAGGA 
GAGCCTGTGT CAGTGAAGCC ACTCCATAGC AGCATCCTTG GACAAGACTT CTGCTTTGAG 
GTTACGTACT TAAGTGGAAG TAAATGCTTC AGCTGTAACT CTGCTTCAGA GAGAGATAAG 
TGGATGGAGA ACCTTCGTAG GACAGTTCAA CCAAATAAGG ACAATTGCAG ACGAGCTGAA 
AACGTTCTTC GTTTATGGAT CATTGAAGCC AAGGACCTTG CCCCCAAAAA GAAATATTTC 
TGTGAACTGT GCCTTGATGA TACCCTCTTT GCTCGTACAA CCAGCAAGAC CAAAGCAGAT 
AATATTTTCT GGGGTGAACA TTTTGAATTT TACAGCCTTC CACCTCTTCA TAGCATCACA 
GTTCACATTT ATAAAGATGT GGAAAAAAAG AAAAAAAAAG ACAAGAATAA TTATGTAGGG 
CTAGTCAACA TTCCCACTGC CAGTGTGACT GGGCGCCAAT TTGTAGAAAA GTGGTATCCA 
GTGAGTACCC CTACACCCAA CAAAGGAAAG ACCGGAGGAC CTTCTATTCG GATTAAATCA 
CGTTTCCAAA CTATCACCAT TCTGCCTATG GAGCAATACA AAGAATTTGC AGAATTTGTC 
ACCAGCAACT ATACCATGCT GTGTTCTGTC CTTGAACCAG TAATTAGTGT GAGAAATAAA 
GAGGAGTTGG CCTGTGCCCT AGTGCACATT CTTCAAAGTA CTGGCAGAGC CAAGGATTTT 
CTGACCGACT TGGTGATGTC TGAGGTGGAT CGCTGTGGAG AGCATGACGT CTTGATCTTC 
AGAGAGAACA CCATTGCTAC CAAATCCATT GAGGAATACC TCAAGTTGGT GGGACAACAG 
TATCTTCATG ACGCATTGGG GGAGTTCATC AAAGCTTTGT ACGAGTCAGA TGAGAACTGT 
GAAGTGGATC CCAGCAAGTG TTCCTCTAGT GAACTGATAG ACCATCAGAG CAACCTGAAG 
ATGTGCTGTG AGCTGGCTTT CTGCAAGATC ATCAACTCTT ACTGTGTTTT CCCTCGTGAG 
TTGAAAGAAG TGTTTGCATC GTGGAAGCAG CAGTGCCTGA ACCGTGGCAA ACAGGACATC 
AGCGAGCGGC TCATCAGTGC CTCATTGTTT CTCCGTTTCC TGTGCCCTGC CATCATGTCT 
CCCAGTCTCT TCAACCTTAT GCAAGAGTAC CCTGATGACC GTACATCTCG CACCCTGACT 
CTGATTGCCA AGGTCATTCA GAACCTGGCT AACTTTGCCA AGTTTGGTAA CAAAGAAGAA 
TATATGGCAT TCATGAATGA TTTTTTAGAA CATGAATGGG GTGGAATGAA GCGCTTTCTT 
TTGGAAATCT CTAATCCGGA CACCATCTCA AACACCCCAG GCTTTGATGG TTACATTGAT 
CTGGGCCGAG AGCTTTCAGT CTTGCATTCC TTACTATGGG AAGTAGTTTC CCAGCTTGAT 
AAGGGTGAAA ATTCCTTCCT ACAGGCGACC GTGGCAAAAT TGGGACCGCT CCCTCGAGTT 
CTTGCTGATA TTACCAAGTC TCTAACTAAT CCTACACCAA TACAACAGCA GCTGAGACGC 
TTCACTGAGC ATAACTCCAG TCCAAATGTC AGTGGAAGCC TCTCCTCTGG GCTGCAGAAA 
ATATTTGAAG ACCCCACTGA CAGTGATTTG CATAAACTAA AATCTCCAAG CCAGGACAAC 
ACAGATGGCT ATTTCAGAGG GAAAACGTTA TTGCTGGTTC AGCAGGCCTC CTCCCAGAGC 
ATGACCTATT CTGAAAAGGA TGAAAAGGAA AGTAGCCTTC CTAACGGTCG GAGCATCTCC 
CTCATGGACC TTCAAGACAC TCACGCTGCT CAAGTAGAGC ATGCATCTAT CATGCTCGAT 
GTGCCTATGC GCTTAACTGG AAGCCAGCTT TCCATAACCC AGGTGGCCAG TATCAAACAA 
CTGCGGGAAA CCCAGAGCAC TCCCCAGAGT GCACCCCAAG TGCGAAGGCC CTTGCACCCA 
GCCTTGAACC AGCCAGGCAG CCTCCAGCCC CTGTCATTCC AGAACCCTGT CTATCACCTC 
AATAACCCAA TCCCAGCAAT GCCAAAGGCC TCTGTGGATT CTAGTTTGGA GAATCTAAGC 
ACTGCCAGTT CCAGAAGCCA AAGTAACAGT GAAGATTTCA AACTCAGTGG GCCCAGCAAT 
AGCAGCATGG AAGATTTCAC TAAACGTAGC ACGCAGAGCG AGGATTTCTC CAGGCGGCAC 
ACTGTACCAG ACAGACACAT ACCTCTTGCT CTGCCGCGGC AGAATAGTAC CGGGCAGGCC 
CAGATCCGGA AAATGGACCA GGCTGGGTTA GGTGCCCGAG CCAAAGCCCC GCCGTCCTTG 
CCACACAGTG CTTCCTTACG TAGCACGGGG AGCATGTCGG TGGCCTCTGC GGCCCTGGTG 
GCTGAACCTG TGCAGAATGG GAGCCGGTCC CGGCAGCAGT CCTCTTCCTC CAGAGAGAGC 
CCCGTTCCCA AAGTGAGAGC GATCCAGAGA CAACAGACAC AGCAGGTGCA GTCACCTGTG 
GACTCTGCCA CCATGTCCCC AGTAGAGAGG ACAGCAGCCT GGGTGTTGAA CAATGGACAG 
TATGAAGAGG ATGTGGAAGA GACTGAGCAA AATCCAGATG AATCCAAGCA TGCTGAGAAG 
TATGAGCAGG AAATTACTAA ACTGAAGGAG CGCCTGAGAG TGTCCAGCCG GCGCCTTGAG 
GAATATGAGC GCCGCTTGCT GGTGCAGGAG CAGCAGATGC AGAAGCTGCT GCTGGAATAC 
AAGGCCAGGC TGGAGGACAG CGAGGAGCGC CTGCGCAGGC AGCAGGAGGA GAAGGACAGC 
CAGATGAAAA GCATCATCAG CAGGCTGATG GCTGTGGAAG AGGAACTGAA GAAGGATCAT 
GCTGAGATGC AAGCAGTTAT TGATGCAAAG CAAAAAATAA TCGATGCACA GGTCATAAAT 
GGAGATGAGA TTATTCAAAC AGGAAAAACG AATCGTGTCC CTGGATTCGG CCAACACCAG 
ACTGATGAGC GCACTGACCC AAGTGAAGGA GCGGTACAGC ATGCAGGTCC GCAATGGCAT 
CTCCCCCACC AACCCCACCA AGCTTTCCAT CACGGAGAAT GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013970636.1
CDS	94..3438
Translation	MDTVNTSPFKVPGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNADDRTRGLPKLKESRSHESLLSPCSAVECLDLGRGEPVSVKPLHSSILGQDFCFEVTYLSGSKCFSCNSASERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYSLPPLHSITVHIYKDVEKKKKKDKNNYVGLVNIPTASVTGRQFVEKWYPVSTPTPNKGKTGGPSIRIKSRFQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEELACALVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTIATKSIEEYLKLVGQQYLHDALGEFIKALYESDENCEVDPSKCSSSELIDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCLNRGKQDISERLISASLFLRFLCPAIMSPSLFNLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNTPGFDGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVAKLGPLPRVLADITKSLTNPTPIQQQLRRFTEHNSSPNVSGSLSSGLQKIFEDPTDSDLHKLKSPSQDNTDGYFRGKTLLLVQQASSQSMTYSEKDEKESSLPNGRSISLMDLQDTHAAQVEHASIMLDVPMRLTGSQLSITQVASIKQLRETQSTPQSAPQVRRPLHPALNQPGSLQPLSFQNPVYHLNNPIPAMPKASVDSSLENLSTASSRSQSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTVPDRHIPLALPRQNSTGQAQIRKMDQAGLGARAKAPPSLPHSASLRSTGSMSVASAALVAEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEDVEETEQNPDESKHAEKYEQEITKLKERLRVSSRRLEEYERRLLVQEQQMQKLLLEYKARLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQVINGDEIIQTGKTNRVPGFGQHQTDERTDPSEGAVQHAGPQWHLPHQPHQAFHHGEW

Target ORF information:

RefSeq Version	XM_014115161.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014115161.1

ORF Insert Sequence:

ATGGACACTG TAAATACCTC ACCCTTCAAA GTACCAGGGT TCTTCAGCAA GCGCCTGAAA 
GGCTCCATCA AGAGGACCAA AAGCCAATCA AAGCTTGACA GAAACACGAG CTTTCGGCTA 
CCATCCCTTC GTAATGCAGA TGACAGGACT CGCGGTCTGC CTAAACTAAA AGAGTCACGT 
TCCCATGAGT CATTGCTGAG CCCTTGTAGT GCAGTGGAAT GTCTGGATCT TGGCAGAGGA 
GAGCCTGTGT CAGTGAAGCC ACTCCATAGC AGCATCCTTG GACAAGACTT CTGCTTTGAG 
GTTACGTACT TAAGTGGAAG TAAATGCTTC AGCTGTAACT CTGCTTCAGA GAGAGATAAG 
TGGATGGAGA ACCTTCGTAG GACAGTTCAA CCAAATAAGG ACAATTGCAG ACGAGCTGAA 
AACGTTCTTC GTTTATGGAT CATTGAAGCC AAGGACCTTG CCCCCAAAAA GAAATATTTC 
TGTGAACTGT GCCTTGATGA TACCCTCTTT GCTCGTACAA CCAGCAAGAC CAAAGCAGAT 
AATATTTTCT GGGGTGAACA TTTTGAATTT TACAGCCTTC CACCTCTTCA TAGCATCACA 
GTTCACATTT ATAAAGATGT GGAAAAAAAG AAAAAAAAAG ACAAGAATAA TTATGTAGGG 
CTAGTCAACA TTCCCACTGC CAGTGTGACT GGGCGCCAAT TTGTAGAAAA GTGGTATCCA 
GTGAGTACCC CTACACCCAA CAAAGGAAAG ACCGGAGGAC CTTCTATTCG GATTAAATCA 
CGTTTCCAAA CTATCACCAT TCTGCCTATG GAGCAATACA AAGAATTTGC AGAATTTGTC 
ACCAGCAACT ATACCATGCT GTGTTCTGTC CTTGAACCAG TAATTAGTGT GAGAAATAAA 
GAGGAGTTGG CCTGTGCCCT AGTGCACATT CTTCAAAGTA CTGGCAGAGC CAAGGATTTT 
CTGACCGACT TGGTGATGTC TGAGGTGGAT CGCTGTGGAG AGCATGACGT CTTGATCTTC 
AGAGAGAACA CCATTGCTAC CAAATCCATT GAGGAATACC TCAAGTTGGT GGGACAACAG 
TATCTTCATG ACGCATTGGG GGAGTTCATC AAAGCTTTGT ACGAGTCAGA TGAGAACTGT 
GAAGTGGATC CCAGCAAGTG TTCCTCTAGT GAACTGATAG ACCATCAGAG CAACCTGAAG 
ATGTGCTGTG AGCTGGCTTT CTGCAAGATC ATCAACTCTT ACTGTGTTTT CCCTCGTGAG 
TTGAAAGAAG TGTTTGCATC GTGGAAGCAG CAGTGCCTGA ACCGTGGCAA ACAGGACATC 
AGCGAGCGGC TCATCAGTGC CTCATTGTTT CTCCGTTTCC TGTGCCCTGC CATCATGTCT 
CCCAGTCTCT TCAACCTTAT GCAAGAGTAC CCTGATGACC GTACATCTCG CACCCTGACT 
CTGATTGCCA AGGTCATTCA GAACCTGGCT AACTTTGCCA AGTTTGGTAA CAAAGAAGAA 
TATATGGCAT TCATGAATGA TTTTTTAGAA CATGAATGGG GTGGAATGAA GCGCTTTCTT 
TTGGAAATCT CTAATCCGGA CACCATCTCA AACACCCCAG GCTTTGATGG TTACATTGAT 
CTGGGCCGAG AGCTTTCAGT CTTGCATTCC TTACTATGGG AAGTAGTTTC CCAGCTTGAT 
AAGGGTGAAA ATTCCTTCCT ACAGGCGACC GTGGCAAAAT TGGGACCGCT CCCTCGAGTT 
CTTGCTGATA TTACCAAGTC TCTAACTAAT CCTACACCAA TACAACAGCA GCTGAGACGC 
TTCACTGAGC ATAACTCCAG TCCAAATGTC AGTGGAAGCC TCTCCTCTGG GCTGCAGAAA 
ATATTTGAAG ACCCCACTGA CAGTGATTTG CATAAACTAA AATCTCCAAG CCAGGACAAC 
ACAGATGGCT ATTTCAGAGG GAAAACGTTA TTGCTGGTTC AGCAGGCCTC CTCCCAGAGC 
ATGACCTATT CTGAAAAGGA TGAAAAGGAA AGTAGCCTTC CTAACGGTCG GAGCATCTCC 
CTCATGGACC TTCAAGACAC TCACGCTGCT CAAGTAGAGC ATGCATCTAT CATGCTCGAT 
GTGCCTATGC GCTTAACTGG AAGCCAGCTT TCCATAACCC AGGTGGCCAG TATCAAACAA 
CTGCGGGAAA CCCAGAGCAC TCCCCAGAGT GCACCCCAAG TGCGAAGGCC CTTGCACCCA 
GCCTTGAACC AGCCAGGCAG CCTCCAGCCC CTGTCATTCC AGAACCCTGT CTATCACCTC 
AATAACCCAA TCCCAGCAAT GCCAAAGGCC TCTGTGGATT CTAGTTTGGA GAATCTAAGC 
ACTGCCAGTT CCAGAAGCCA AAGTAACAGT GAAGATTTCA AACTCAGTGG GCCCAGCAAT 
AGCAGCATGG AAGATTTCAC TAAACGTAGC ACGCAGAGCG AGGATTTCTC CAGGCGGCAC 
ACTGTACCAG ACAGACACAT ACCTCTTGCT CTGCCGCGGC AGAATAGTAC CGGGCAGGCC 
CAGATCCGGA AAATGGACCA GGCTGGGTTA GGTGCCCGAG CCAAAGCCCC GCCGTCCTTG 
CCACACAGTG CTTCCTTACG TAGCACGGGG AGCATGTCGG TGGCCTCTGC GGCCCTGGTG 
GCTGAACCTG TGCAGAATGG GAGCCGGTCC CGGCAGCAGT CCTCTTCCTC CAGAGAGAGC 
CCCGTTCCCA AAGTGAGAGC GATCCAGAGA CAACAGACAC AGCAGGTGCA GTCACCTGTG 
GACTCTGCCA CCATGTCCCC AGTAGAGAGG ACAGCAGCCT GGGTGTTGAA CAATGGACAG 
TATGAAGAGG ATGTGGAAGA GACTGAGCAA AATCCAGATG AATCCAAGCA TGCTGAGAAG 
TATGAGCAGG AAATTACTAA ACTGAAGGAG CGCCTGAGAG TGTCCAGCCG GCGCCTTGAG 
GAATATGAGC GCCGCTTGCT GGTGCAGGAG CAGCAGATGC AGAAGCTGCT GCTGGAATAC 
AAGGCCAGGC TGGAGGACAG CGAGGAGCGC CTGCGCAGGC AGCAGGAGGA GAAGGACAGC 
CAGATGAAAA GCATCATCAG CAGGCTGATG GCTGTGGAAG AGGAACTGAA GAAGGATCAT 
GCTGAGATGC AAGCAGTTAT TGATGCAAAG CAAAAAATAA TCGATGCACA GGTCATAAAT 
GGAGATGAGA TTATTCAAAC AGGAAAAACG AATCGTGTCC CTGGATTCGG CCAACACCAG 
ACTGATGAGC GCACTGACCC AAGTGAAGGA GCGGTACAGC ATGCAGGTCC GCAATGGCAT 
CTCCCCCACC AACCCCACCA AGCTTTCCAT CACGGAGAAT GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_005622480.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005622537.1 Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X5, mRNA.

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CloneID	OCe09201
Clone ID Related Accession (Same CDS sequence)	XM_005622480.2 , XM_005622480.3
Accession Version	XM_005622480.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3864bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	ras GTPase-activating protein nGAP isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005622480.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTCT CCCCGTCGTC CGGAGGAGCC GCCGAGGCGC TGTCCTGGCC GGACATGTTC 
CCGGCGCTGG AGTCCGACTC GCCGATACCG CCGGAGGAGC TGGAAGCGGT TCTCCCCGTC 
GGCGGGGCCG TGGCCGGGGG CATGCTGGAT CGGATCCTTC TGGAGTCGGT GTGCCAGCAG 
CAGAGCTGGG TCCGGGTGTA CGATGTGAAG GGGCCACCTA CCCATCGTCT GTCTTGTGGA 
CAGTCGCCCT ACACAGAGAC GACAACATGG GAGCGGAAGT ATTGCATCCT CACCGACAGC 
CAGTTGGTGT TGCTCAACAA GGAGAAGGAG ATACCTGTGG AGGGAGGACA GGAGCAGCAG 
ACAGATTCCA CCAAAGGGCG ATGCCTGAGG AGAACCGTCA GTGTCCCTTC CGAGGGCCAG 
TTTCCTGAGT ACCCACCAGA GGGCGCCACC AAACTGGAGG TGCCGGCAGA AAGGTCCCCA 
CGCAGACGGA GTATCTCAGG GACCAGTACA TCAGAGAAAC CCAACTCCAT GGACACTGTA 
AATACCTCAC CCTTCAAAGT ACCAGGGTTC TTCAGCAAGC GCCTGAAAGG CTCCATCAAG 
AGGACCAAAA GCCAATCAAA GCTTGACAGA AACACGAGCT TTCGGCTACC ATCCCTTCGT 
AATGCAGATG ACAGGACTCG CGGTCTGCCT AAACTAAAAG AGTCACGTTC CCATGAGTCA 
TTGCTGAGCC CTTGTAGTGC AGTGGAATGT CTGGATCTTG GCAGAGGAGA GCCTGTGTCA 
GTGAAGCCAC TCCATAGCAG CATCCTTGGA CAAGACTTCT GCTTTGAGGT TACGTACTTA 
AGTGGAAGTA AATGCTTCAG CTGTAACTCT GCTTCAGAGA GAGATAAGTG GATGGAGAAC 
CTTCGTAGGA CAGTTCAACC AAATAAGGAC AATTGCAGAC GAGCTGAAAA CGTTCTTCGT 
TTATGGATCA TTGAAGCCAA GGACCTTGCC CCCAAAAAGA AATATTTCTG TGAACTGTGC 
CTTGATGATA CCCTCTTTGC TCGTACAACC AGCAAGACCA AAGCAGATAA TATTTTCTGG 
GGTGAACATT TTGAATTTTA CAGCCTTCCA CCTCTTCATA GCATCACAGT TCACATTTAT 
AAAGATGTGG AAAAAAAGAA AAAAAAAGAC AAGAATAATT ATGTAGGGCT AGTCAACATT 
CCCACTGCCA GTGTGACTGG GCGCCAATTT GTAGAAAAGT GGTATCCAGT GAGTACCCCT 
ACACCCAACA AAGGAAAGAC CGGAGGACCT TCTATTCGGA TTAAATCACG TTTCCAAACT 
ATCACCATTC TGCCTATGGA GCAATACAAA GAATTTGCAG AATTTGTCAC CAGCAACTAT 
ACCATGCTGT GTTCTGTCCT TGAACCAGTA ATTAGTGTGA GAAATAAAGA GGAGTTGGCC 
TGTGCCCTAG TGCACATTCT TCAAAGTACT GGCAGAGCCA AGGATTTTCT GACCGACTTG 
GTGATGTCTG AGGTGGATCG CTGTGGAGAG CATGACGTCT TGATCTTCAG AGAGAACACC 
ATTGCTACCA AATCCATTGA GGAATACCTC AAGTTGGTGG GACAACAGTA TCTTCATGAC 
GCATTGGGGG AGTTCATCAA AGCTTTGTAC GAGTCAGATG AGAACTGTGA AGTGGATCCC 
AGCAAGTGTT CCTCTAGTGA ACTGATAGAC CATCAGAGCA ACCTGAAGAT GTGCTGTGAG 
CTGGCTTTCT GCAAGATCAT CAACTCTTAC TGTGTTTTCC CTCGTGAGTT GAAAGAAGTG 
TTTGCATCGT GGAAGCAGCA GTGCCTGAAC CGTGGCAAAC AGGACATCAG CGAGCGGCTC 
ATCAGTGCCT CATTGTTTCT CCGTTTCCTG TGCCCTGCCA TCATGTCTCC CAGTCTCTTC 
AACCTTATGC AAGAGTACCC TGATGACCGT ACATCTCGCA CCCTGACTCT GATTGCCAAG 
GTCATTCAGA ACCTGGCTAA CTTTGCCAAG TTTGGTAACA AAGAAGAATA TATGGCATTC 
ATGAATGATT TTTTAGAACA TGAATGGGGT GGAATGAAGC GCTTTCTTTT GGAAATCTCT 
AATCCGGACA CCATCTCAAA CACCCCAGGC TTTGATGGTT ACATTGATCT GGGCCGAGAG 
CTTTCAGTCT TGCATTCCTT ACTATGGGAA GTAGTTTCCC AGCTTGATAA GGGTGAAAAT 
TCCTTCCTAC AGGCGACCGT GGCAAAATTG GGACCGCTCC CTCGAGTTCT TGCTGATATT 
ACCAAGTCTC TAACTAATCC TACACCAATA CAACAGCAGC TGAGACGCTT CACTGAGCAT 
AACTCCAGTC CAAATGTCAG TGGAAGCCTC TCCTCTGGGC TGCAGAAAAT ATTTGAAGAC 
CCCACTGACA GTGATTTGCA TAAACTAAAA TCTCCAAGCC AGGACAACAC AGATGGCTAT 
TTCAGAGGGA AAACGTTATT GCTGGTTCAG CAGGCCTCCT CCCAGAGCAT GACCTATTCT 
GAAAAGGATG AAAAGGAAAG TAGCCTTCCT AACGGTCGGA GCATCTCCCT CATGGACCTT 
CAAGACACTC ACGCTGCTCA AGTAGAGCAT GCATCTATCA TGCTCGATGT GCCTATGCGC 
TTAACTGGAA GCCAGCTTTC CATAACCCAG GTGGCCAGTA TCAAACAACT GCGGGAAACC 
CAGAGCACTC CCCAGAGTGC ACCCCAAGTG CGAAGGCCCT TGCACCCAGC CTTGAACCAG 
CCAGGCAGCC TCCAGCCCCT GTCATTCCAG AACCCTGTCT ATCACCTCAA TAACCCAATC 
CCAGCAATGC CAAAGGCCTC TGTGGATTCT AGTTTGGAGA ATCTAAGCAC TGCCAGTTCC 
AGAAGCCAAA GTAACAGTGA AGATTTCAAA CTCAGTGGGC CCAGCAATAG CAGCATGGAA 
GATTTCACTA AACGTAGCAC GCAGAGCGAG GATTTCTCCA GGCGGCACAC TGTACCAGAC 
AGACACATAC CTCTTGCTCT GCCGCGGCAG AATAGTACCG GGCAGGCCCA GATCCGGAAA 
ATGGACCAGG CTGGGTTAGG TGCCCGAGCC AAAGCCCCGC CGTCCTTGCC ACACAGTGCT 
TCCTTACGTA GCACGGGGAG CATGTCGGTG GCCTCTGCGG CCCTGGTGGC TGAACCTGTG 
CAGAATGGGA GCCGGTCCCG GCAGCAGTCC TCTTCCTCCA GAGAGAGCCC CGTTCCCAAA 
GTGAGAGCGA TCCAGAGACA ACAGACACAG CAGGTGCAGT CACCTGTGGA CTCTGCCACC 
ATGTCCCCAG TAGAGAGGAC AGCAGCCTGG GTGTTGAACA ATGGACAGTA TGAAGAGGAT 
GTGGAAGAGA CTGAGCAAAA TCCAGATGAA TCCAAGCATG CTGAGAAGTA TGAGCAGGAA 
ATTACTAAAC TGAAGGAGCG CCTGAGAGTG TCCAGCCGGC GCCTTGAGGA ATATGAGCGC 
CGCTTGCTGG TGCAGGAGCA GCAGATGCAG AAGCTGCTGC TGGAATACAA GGCCAGGCTG 
GAGGACAGCG AGGAGCGCCT GCGCAGGCAG CAGGAGGAGA AGGACAGCCA GATGAAAAGC 
ATCATCAGCA GGCTGATGGC TGTGGAAGAG GAACTGAAGA AGGATCATGC TGAGATGCAA 
GCAGTTATTG ATGCAAAGCA AAAAATAATC GATGCACAGG AAAAACGAAT CGTGTCCCTG 
GATTCGGCCA ACACCAGACT GATGAGCGCA CTGACCCAAG TGAAGGAGCG GTACAGCATG 
CAGGTCCGCA ATGGCATCTC CCCCACCAAC CCCACCAAGC TTTCCATCAC GGAGAATGGT 
GAATTCAAAA ACAGCAGCTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005622537.1
CDS	54..3917
Translation	MELSPSSGGAAEALSWPDMFPALESDSPIPPEELEAVLPVGGAVAGGMLDRILLESVCQQQSWVRVYDVKGPPTHRLSCGQSPYTETTTWERKYCILTDSQLVLLNKEKEIPVEGGQEQQTDSTKGRCLRRTVSVPSEGQFPEYPPEGATKLEVPAERSPRRRSISGTSTSEKPNSMDTVNTSPFKVPGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNADDRTRGLPKLKESRSHESLLSPCSAVECLDLGRGEPVSVKPLHSSILGQDFCFEVTYLSGSKCFSCNSASERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYSLPPLHSITVHIYKDVEKKKKKDKNNYVGLVNIPTASVTGRQFVEKWYPVSTPTPNKGKTGGPSIRIKSRFQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEELACALVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTIATKSIEEYLKLVGQQYLHDALGEFIKALYESDENCEVDPSKCSSSELIDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCLNRGKQDISERLISASLFLRFLCPAIMSPSLFNLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNTPGFDGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVAKLGPLPRVLADITKSLTNPTPIQQQLRRFTEHNSSPNVSGSLSSGLQKIFEDPTDSDLHKLKSPSQDNTDGYFRGKTLLLVQQASSQSMTYSEKDEKESSLPNGRSISLMDLQDTHAAQVEHASIMLDVPMRLTGSQLSITQVASIKQLRETQSTPQSAPQVRRPLHPALNQPGSLQPLSFQNPVYHLNNPIPAMPKASVDSSLENLSTASSRSQSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTVPDRHIPLALPRQNSTGQAQIRKMDQAGLGARAKAPPSLPHSASLRSTGSMSVASAALVAEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEDVEETEQNPDESKHAEKYEQEITKLKERLRVSSRRLEEYERRLLVQEQQMQKLLLEYKARLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQEKRIVSLDSANTRLMSALTQVKERYSMQVRNGISPTNPTKLSITENGEFKNSSC

Target ORF information:

RefSeq Version	XM_005622480.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005622480.2

ORF Insert Sequence:

ATGGAGCTCT CCCCGTCGTC CGGAGGAGCC GCCGAGGCGC TGTCCTGGCC GGACATGTTC 
CCGGCGCTGG AGTCCGACTC GCCGATACCG CCGGAGGAGC TGGAAGCGGT TCTCCCCGTC 
GGCGGGGCCG TGGCCGGGGG CATGCTGGAT CGGATCCTTC TGGAGTCGGT GTGCCAGCAG 
CAGAGCTGGG TCCGGGTGTA CGATGTGAAG GGGCCACCTA CCCATCGTCT GTCTTGTGGA 
CAGTCGCCCT ACACAGAGAC GACAACATGG GAGCGGAAGT ATTGCATCCT CACCGACAGC 
CAGTTGGTGT TGCTCAACAA GGAGAAGGAG ATACCTGTGG AGGGAGGACA GGAGCAGCAG 
ACAGATTCCA CCAAAGGGCG ATGCCTGAGG AGAACCGTCA GTGTCCCTTC CGAGGGCCAG 
TTTCCTGAGT ACCCACCAGA GGGCGCCACC AAACTGGAGG TGCCGGCAGA AAGGTCCCCA 
CGCAGACGGA GTATCTCAGG GACCAGTACA TCAGAGAAAC CCAACTCCAT GGACACTGTA 
AATACCTCAC CCTTCAAAGT ACCAGGGTTC TTCAGCAAGC GCCTGAAAGG CTCCATCAAG 
AGGACCAAAA GCCAATCAAA GCTTGACAGA AACACGAGCT TTCGGCTACC ATCCCTTCGT 
AATGCAGATG ACAGGACTCG CGGTCTGCCT AAACTAAAAG AGTCACGTTC CCATGAGTCA 
TTGCTGAGCC CTTGTAGTGC AGTGGAATGT CTGGATCTTG GCAGAGGAGA GCCTGTGTCA 
GTGAAGCCAC TCCATAGCAG CATCCTTGGA CAAGACTTCT GCTTTGAGGT TACGTACTTA 
AGTGGAAGTA AATGCTTCAG CTGTAACTCT GCTTCAGAGA GAGATAAGTG GATGGAGAAC 
CTTCGTAGGA CAGTTCAACC AAATAAGGAC AATTGCAGAC GAGCTGAAAA CGTTCTTCGT 
TTATGGATCA TTGAAGCCAA GGACCTTGCC CCCAAAAAGA AATATTTCTG TGAACTGTGC 
CTTGATGATA CCCTCTTTGC TCGTACAACC AGCAAGACCA AAGCAGATAA TATTTTCTGG 
GGTGAACATT TTGAATTTTA CAGCCTTCCA CCTCTTCATA GCATCACAGT TCACATTTAT 
AAAGATGTGG AAAAAAAGAA AAAAAAAGAC AAGAATAATT ATGTAGGGCT AGTCAACATT 
CCCACTGCCA GTGTGACTGG GCGCCAATTT GTAGAAAAGT GGTATCCAGT GAGTACCCCT 
ACACCCAACA AAGGAAAGAC CGGAGGACCT TCTATTCGGA TTAAATCACG TTTCCAAACT 
ATCACCATTC TGCCTATGGA GCAATACAAA GAATTTGCAG AATTTGTCAC CAGCAACTAT 
ACCATGCTGT GTTCTGTCCT TGAACCAGTA ATTAGTGTGA GAAATAAAGA GGAGTTGGCC 
TGTGCCCTAG TGCACATTCT TCAAAGTACT GGCAGAGCCA AGGATTTTCT GACCGACTTG 
GTGATGTCTG AGGTGGATCG CTGTGGAGAG CATGACGTCT TGATCTTCAG AGAGAACACC 
ATTGCTACCA AATCCATTGA GGAATACCTC AAGTTGGTGG GACAACAGTA TCTTCATGAC 
GCATTGGGGG AGTTCATCAA AGCTTTGTAC GAGTCAGATG AGAACTGTGA AGTGGATCCC 
AGCAAGTGTT CCTCTAGTGA ACTGATAGAC CATCAGAGCA ACCTGAAGAT GTGCTGTGAG 
CTGGCTTTCT GCAAGATCAT CAACTCTTAC TGTGTTTTCC CTCGTGAGTT GAAAGAAGTG 
TTTGCATCGT GGAAGCAGCA GTGCCTGAAC CGTGGCAAAC AGGACATCAG CGAGCGGCTC 
ATCAGTGCCT CATTGTTTCT CCGTTTCCTG TGCCCTGCCA TCATGTCTCC CAGTCTCTTC 
AACCTTATGC AAGAGTACCC TGATGACCGT ACATCTCGCA CCCTGACTCT GATTGCCAAG 
GTCATTCAGA ACCTGGCTAA CTTTGCCAAG TTTGGTAACA AAGAAGAATA TATGGCATTC 
ATGAATGATT TTTTAGAACA TGAATGGGGT GGAATGAAGC GCTTTCTTTT GGAAATCTCT 
AATCCGGACA CCATCTCAAA CACCCCAGGC TTTGATGGTT ACATTGATCT GGGCCGAGAG 
CTTTCAGTCT TGCATTCCTT ACTATGGGAA GTAGTTTCCC AGCTTGATAA GGGTGAAAAT 
TCCTTCCTAC AGGCGACCGT GGCAAAATTG GGACCGCTCC CTCGAGTTCT TGCTGATATT 
ACCAAGTCTC TAACTAATCC TACACCAATA CAACAGCAGC TGAGACGCTT CACTGAGCAT 
AACTCCAGTC CAAATGTCAG TGGAAGCCTC TCCTCTGGGC TGCAGAAAAT ATTTGAAGAC 
CCCACTGACA GTGATTTGCA TAAACTAAAA TCTCCAAGCC AGGACAACAC AGATGGCTAT 
TTCAGAGGGA AAACGTTATT GCTGGTTCAG CAGGCCTCCT CCCAGAGCAT GACCTATTCT 
GAAAAGGATG AAAAGGAAAG TAGCCTTCCT AACGGTCGGA GCATCTCCCT CATGGACCTT 
CAAGACACTC ACGCTGCTCA AGTAGAGCAT GCATCTATCA TGCTCGATGT GCCTATGCGC 
TTAACTGGAA GCCAGCTTTC CATAACCCAG GTGGCCAGTA TCAAACAACT GCGGGAAACC 
CAGAGCACTC CCCAGAGTGC ACCCCAAGTG CGAAGGCCCT TGCACCCAGC CTTGAACCAG 
CCAGGCAGCC TCCAGCCCCT GTCATTCCAG AACCCTGTCT ATCACCTCAA TAACCCAATC 
CCAGCAATGC CAAAGGCCTC TGTGGATTCT AGTTTGGAGA ATCTAAGCAC TGCCAGTTCC 
AGAAGCCAAA GTAACAGTGA AGATTTCAAA CTCAGTGGGC CCAGCAATAG CAGCATGGAA 
GATTTCACTA AACGTAGCAC GCAGAGCGAG GATTTCTCCA GGCGGCACAC TGTACCAGAC 
AGACACATAC CTCTTGCTCT GCCGCGGCAG AATAGTACCG GGCAGGCCCA GATCCGGAAA 
ATGGACCAGG CTGGGTTAGG TGCCCGAGCC AAAGCCCCGC CGTCCTTGCC ACACAGTGCT 
TCCTTACGTA GCACGGGGAG CATGTCGGTG GCCTCTGCGG CCCTGGTGGC TGAACCTGTG 
CAGAATGGGA GCCGGTCCCG GCAGCAGTCC TCTTCCTCCA GAGAGAGCCC CGTTCCCAAA 
GTGAGAGCGA TCCAGAGACA ACAGACACAG CAGGTGCAGT CACCTGTGGA CTCTGCCACC 
ATGTCCCCAG TAGAGAGGAC AGCAGCCTGG GTGTTGAACA ATGGACAGTA TGAAGAGGAT 
GTGGAAGAGA CTGAGCAAAA TCCAGATGAA TCCAAGCATG CTGAGAAGTA TGAGCAGGAA 
ATTACTAAAC TGAAGGAGCG CCTGAGAGTG TCCAGCCGGC GCCTTGAGGA ATATGAGCGC 
CGCTTGCTGG TGCAGGAGCA GCAGATGCAG AAGCTGCTGC TGGAATACAA GGCCAGGCTG 
GAGGACAGCG AGGAGCGCCT GCGCAGGCAG CAGGAGGAGA AGGACAGCCA GATGAAAAGC 
ATCATCAGCA GGCTGATGGC TGTGGAAGAG GAACTGAAGA AGGATCATGC TGAGATGCAA 
GCAGTTATTG ATGCAAAGCA AAAAATAATC GATGCACAGG AAAAACGAAT CGTGTCCCTG 
GATTCGGCCA ACACCAGACT GATGAGCGCA CTGACCCAAG TGAAGGAGCG GTACAGCATG 
CAGGTCCGCA ATGGCATCTC CCCCACCAAC CCCACCAAGC TTTCCATCAC GGAGAATGGT 
GAATTCAAAA ACAGCAGCTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_005622480.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005622537.1 Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OCe09201
Clone ID Related Accession (Same CDS sequence)	XM_005622480.2 , XM_005622480.3
Accession Version	XM_005622480.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3864bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	ras GTPase-activating protein nGAP isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005622480.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTCT CCCCGTCGTC CGGAGGAGCC GCCGAGGCGC TGTCCTGGCC GGACATGTTC 
CCGGCGCTGG AGTCCGACTC GCCGATACCG CCGGAGGAGC TGGAAGCGGT TCTCCCCGTC 
GGCGGGGCCG TGGCCGGGGG CATGCTGGAT CGGATCCTTC TGGAGTCGGT GTGCCAGCAG 
CAGAGCTGGG TCCGGGTGTA CGATGTGAAG GGGCCACCTA CCCATCGTCT GTCTTGTGGA 
CAGTCGCCCT ACACAGAGAC GACAACATGG GAGCGGAAGT ATTGCATCCT CACCGACAGC 
CAGTTGGTGT TGCTCAACAA GGAGAAGGAG ATACCTGTGG AGGGAGGACA GGAGCAGCAG 
ACAGATTCCA CCAAAGGGCG ATGCCTGAGG AGAACCGTCA GTGTCCCTTC CGAGGGCCAG 
TTTCCTGAGT ACCCACCAGA GGGCGCCACC AAACTGGAGG TGCCGGCAGA AAGGTCCCCA 
CGCAGACGGA GTATCTCAGG GACCAGTACA TCAGAGAAAC CCAACTCCAT GGACACTGTA 
AATACCTCAC CCTTCAAAGT ACCAGGGTTC TTCAGCAAGC GCCTGAAAGG CTCCATCAAG 
AGGACCAAAA GCCAATCAAA GCTTGACAGA AACACGAGCT TTCGGCTACC ATCCCTTCGT 
AATGCAGATG ACAGGACTCG CGGTCTGCCT AAACTAAAAG AGTCACGTTC CCATGAGTCA 
TTGCTGAGCC CTTGTAGTGC AGTGGAATGT CTGGATCTTG GCAGAGGAGA GCCTGTGTCA 
GTGAAGCCAC TCCATAGCAG CATCCTTGGA CAAGACTTCT GCTTTGAGGT TACGTACTTA 
AGTGGAAGTA AATGCTTCAG CTGTAACTCT GCTTCAGAGA GAGATAAGTG GATGGAGAAC 
CTTCGTAGGA CAGTTCAACC AAATAAGGAC AATTGCAGAC GAGCTGAAAA CGTTCTTCGT 
TTATGGATCA TTGAAGCCAA GGACCTTGCC CCCAAAAAGA AATATTTCTG TGAACTGTGC 
CTTGATGATA CCCTCTTTGC TCGTACAACC AGCAAGACCA AAGCAGATAA TATTTTCTGG 
GGTGAACATT TTGAATTTTA CAGCCTTCCA CCTCTTCATA GCATCACAGT TCACATTTAT 
AAAGATGTGG AAAAAAAGAA AAAAAAAGAC AAGAATAATT ATGTAGGGCT AGTCAACATT 
CCCACTGCCA GTGTGACTGG GCGCCAATTT GTAGAAAAGT GGTATCCAGT GAGTACCCCT 
ACACCCAACA AAGGAAAGAC CGGAGGACCT TCTATTCGGA TTAAATCACG TTTCCAAACT 
ATCACCATTC TGCCTATGGA GCAATACAAA GAATTTGCAG AATTTGTCAC CAGCAACTAT 
ACCATGCTGT GTTCTGTCCT TGAACCAGTA ATTAGTGTGA GAAATAAAGA GGAGTTGGCC 
TGTGCCCTAG TGCACATTCT TCAAAGTACT GGCAGAGCCA AGGATTTTCT GACCGACTTG 
GTGATGTCTG AGGTGGATCG CTGTGGAGAG CATGACGTCT TGATCTTCAG AGAGAACACC 
ATTGCTACCA AATCCATTGA GGAATACCTC AAGTTGGTGG GACAACAGTA TCTTCATGAC 
GCATTGGGGG AGTTCATCAA AGCTTTGTAC GAGTCAGATG AGAACTGTGA AGTGGATCCC 
AGCAAGTGTT CCTCTAGTGA ACTGATAGAC CATCAGAGCA ACCTGAAGAT GTGCTGTGAG 
CTGGCTTTCT GCAAGATCAT CAACTCTTAC TGTGTTTTCC CTCGTGAGTT GAAAGAAGTG 
TTTGCATCGT GGAAGCAGCA GTGCCTGAAC CGTGGCAAAC AGGACATCAG CGAGCGGCTC 
ATCAGTGCCT CATTGTTTCT CCGTTTCCTG TGCCCTGCCA TCATGTCTCC CAGTCTCTTC 
AACCTTATGC AAGAGTACCC TGATGACCGT ACATCTCGCA CCCTGACTCT GATTGCCAAG 
GTCATTCAGA ACCTGGCTAA CTTTGCCAAG TTTGGTAACA AAGAAGAATA TATGGCATTC 
ATGAATGATT TTTTAGAACA TGAATGGGGT GGAATGAAGC GCTTTCTTTT GGAAATCTCT 
AATCCGGACA CCATCTCAAA CACCCCAGGC TTTGATGGTT ACATTGATCT GGGCCGAGAG 
CTTTCAGTCT TGCATTCCTT ACTATGGGAA GTAGTTTCCC AGCTTGATAA GGGTGAAAAT 
TCCTTCCTAC AGGCGACCGT GGCAAAATTG GGACCGCTCC CTCGAGTTCT TGCTGATATT 
ACCAAGTCTC TAACTAATCC TACACCAATA CAACAGCAGC TGAGACGCTT CACTGAGCAT 
AACTCCAGTC CAAATGTCAG TGGAAGCCTC TCCTCTGGGC TGCAGAAAAT ATTTGAAGAC 
CCCACTGACA GTGATTTGCA TAAACTAAAA TCTCCAAGCC AGGACAACAC AGATGGCTAT 
TTCAGAGGGA AAACGTTATT GCTGGTTCAG CAGGCCTCCT CCCAGAGCAT GACCTATTCT 
GAAAAGGATG AAAAGGAAAG TAGCCTTCCT AACGGTCGGA GCATCTCCCT CATGGACCTT 
CAAGACACTC ACGCTGCTCA AGTAGAGCAT GCATCTATCA TGCTCGATGT GCCTATGCGC 
TTAACTGGAA GCCAGCTTTC CATAACCCAG GTGGCCAGTA TCAAACAACT GCGGGAAACC 
CAGAGCACTC CCCAGAGTGC ACCCCAAGTG CGAAGGCCCT TGCACCCAGC CTTGAACCAG 
CCAGGCAGCC TCCAGCCCCT GTCATTCCAG AACCCTGTCT ATCACCTCAA TAACCCAATC 
CCAGCAATGC CAAAGGCCTC TGTGGATTCT AGTTTGGAGA ATCTAAGCAC TGCCAGTTCC 
AGAAGCCAAA GTAACAGTGA AGATTTCAAA CTCAGTGGGC CCAGCAATAG CAGCATGGAA 
GATTTCACTA AACGTAGCAC GCAGAGCGAG GATTTCTCCA GGCGGCACAC TGTACCAGAC 
AGACACATAC CTCTTGCTCT GCCGCGGCAG AATAGTACCG GGCAGGCCCA GATCCGGAAA 
ATGGACCAGG CTGGGTTAGG TGCCCGAGCC AAAGCCCCGC CGTCCTTGCC ACACAGTGCT 
TCCTTACGTA GCACGGGGAG CATGTCGGTG GCCTCTGCGG CCCTGGTGGC TGAACCTGTG 
CAGAATGGGA GCCGGTCCCG GCAGCAGTCC TCTTCCTCCA GAGAGAGCCC CGTTCCCAAA 
GTGAGAGCGA TCCAGAGACA ACAGACACAG CAGGTGCAGT CACCTGTGGA CTCTGCCACC 
ATGTCCCCAG TAGAGAGGAC AGCAGCCTGG GTGTTGAACA ATGGACAGTA TGAAGAGGAT 
GTGGAAGAGA CTGAGCAAAA TCCAGATGAA TCCAAGCATG CTGAGAAGTA TGAGCAGGAA 
ATTACTAAAC TGAAGGAGCG CCTGAGAGTG TCCAGCCGGC GCCTTGAGGA ATATGAGCGC 
CGCTTGCTGG TGCAGGAGCA GCAGATGCAG AAGCTGCTGC TGGAATACAA GGCCAGGCTG 
GAGGACAGCG AGGAGCGCCT GCGCAGGCAG CAGGAGGAGA AGGACAGCCA GATGAAAAGC 
ATCATCAGCA GGCTGATGGC TGTGGAAGAG GAACTGAAGA AGGATCATGC TGAGATGCAA 
GCAGTTATTG ATGCAAAGCA AAAAATAATC GATGCACAGG AAAAACGAAT CGTGTCCCTG 
GATTCGGCCA ACACCAGACT GATGAGCGCA CTGACCCAAG TGAAGGAGCG GTACAGCATG 
CAGGTCCGCA ATGGCATCTC CCCCACCAAC CCCACCAAGC TTTCCATCAC GGAGAATGGT 
GAATTCAAAA ACAGCAGCTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005622537.1
CDS	34..3897
Translation	MELSPSSGGAAEALSWPDMFPALESDSPIPPEELEAVLPVGGAVAGGMLDRILLESVCQQQSWVRVYDVKGPPTHRLSCGQSPYTETTTWERKYCILTDSQLVLLNKEKEIPVEGGQEQQTDSTKGRCLRRTVSVPSEGQFPEYPPEGATKLEVPAERSPRRRSISGTSTSEKPNSMDTVNTSPFKVPGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNADDRTRGLPKLKESRSHESLLSPCSAVECLDLGRGEPVSVKPLHSSILGQDFCFEVTYLSGSKCFSCNSASERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYSLPPLHSITVHIYKDVEKKKKKDKNNYVGLVNIPTASVTGRQFVEKWYPVSTPTPNKGKTGGPSIRIKSRFQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEELACALVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTIATKSIEEYLKLVGQQYLHDALGEFIKALYESDENCEVDPSKCSSSELIDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCLNRGKQDISERLISASLFLRFLCPAIMSPSLFNLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNTPGFDGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVAKLGPLPRVLADITKSLTNPTPIQQQLRRFTEHNSSPNVSGSLSSGLQKIFEDPTDSDLHKLKSPSQDNTDGYFRGKTLLLVQQASSQSMTYSEKDEKESSLPNGRSISLMDLQDTHAAQVEHASIMLDVPMRLTGSQLSITQVASIKQLRETQSTPQSAPQVRRPLHPALNQPGSLQPLSFQNPVYHLNNPIPAMPKASVDSSLENLSTASSRSQSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTVPDRHIPLALPRQNSTGQAQIRKMDQAGLGARAKAPPSLPHSASLRSTGSMSVASAALVAEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEDVEETEQNPDESKHAEKYEQEITKLKERLRVSSRRLEEYERRLLVQEQQMQKLLLEYKARLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQEKRIVSLDSANTRLMSALTQVKERYSMQVRNGISPTNPTKLSITENGEFKNSSC

Target ORF information:

RefSeq Version	XM_005622480.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005622480.3

ORF Insert Sequence:

ATGGAGCTCT CCCCGTCGTC CGGAGGAGCC GCCGAGGCGC TGTCCTGGCC GGACATGTTC 
CCGGCGCTGG AGTCCGACTC GCCGATACCG CCGGAGGAGC TGGAAGCGGT TCTCCCCGTC 
GGCGGGGCCG TGGCCGGGGG CATGCTGGAT CGGATCCTTC TGGAGTCGGT GTGCCAGCAG 
CAGAGCTGGG TCCGGGTGTA CGATGTGAAG GGGCCACCTA CCCATCGTCT GTCTTGTGGA 
CAGTCGCCCT ACACAGAGAC GACAACATGG GAGCGGAAGT ATTGCATCCT CACCGACAGC 
CAGTTGGTGT TGCTCAACAA GGAGAAGGAG ATACCTGTGG AGGGAGGACA GGAGCAGCAG 
ACAGATTCCA CCAAAGGGCG ATGCCTGAGG AGAACCGTCA GTGTCCCTTC CGAGGGCCAG 
TTTCCTGAGT ACCCACCAGA GGGCGCCACC AAACTGGAGG TGCCGGCAGA AAGGTCCCCA 
CGCAGACGGA GTATCTCAGG GACCAGTACA TCAGAGAAAC CCAACTCCAT GGACACTGTA 
AATACCTCAC CCTTCAAAGT ACCAGGGTTC TTCAGCAAGC GCCTGAAAGG CTCCATCAAG 
AGGACCAAAA GCCAATCAAA GCTTGACAGA AACACGAGCT TTCGGCTACC ATCCCTTCGT 
AATGCAGATG ACAGGACTCG CGGTCTGCCT AAACTAAAAG AGTCACGTTC CCATGAGTCA 
TTGCTGAGCC CTTGTAGTGC AGTGGAATGT CTGGATCTTG GCAGAGGAGA GCCTGTGTCA 
GTGAAGCCAC TCCATAGCAG CATCCTTGGA CAAGACTTCT GCTTTGAGGT TACGTACTTA 
AGTGGAAGTA AATGCTTCAG CTGTAACTCT GCTTCAGAGA GAGATAAGTG GATGGAGAAC 
CTTCGTAGGA CAGTTCAACC AAATAAGGAC AATTGCAGAC GAGCTGAAAA CGTTCTTCGT 
TTATGGATCA TTGAAGCCAA GGACCTTGCC CCCAAAAAGA AATATTTCTG TGAACTGTGC 
CTTGATGATA CCCTCTTTGC TCGTACAACC AGCAAGACCA AAGCAGATAA TATTTTCTGG 
GGTGAACATT TTGAATTTTA CAGCCTTCCA CCTCTTCATA GCATCACAGT TCACATTTAT 
AAAGATGTGG AAAAAAAGAA AAAAAAAGAC AAGAATAATT ATGTAGGGCT AGTCAACATT 
CCCACTGCCA GTGTGACTGG GCGCCAATTT GTAGAAAAGT GGTATCCAGT GAGTACCCCT 
ACACCCAACA AAGGAAAGAC CGGAGGACCT TCTATTCGGA TTAAATCACG TTTCCAAACT 
ATCACCATTC TGCCTATGGA GCAATACAAA GAATTTGCAG AATTTGTCAC CAGCAACTAT 
ACCATGCTGT GTTCTGTCCT TGAACCAGTA ATTAGTGTGA GAAATAAAGA GGAGTTGGCC 
TGTGCCCTAG TGCACATTCT TCAAAGTACT GGCAGAGCCA AGGATTTTCT GACCGACTTG 
GTGATGTCTG AGGTGGATCG CTGTGGAGAG CATGACGTCT TGATCTTCAG AGAGAACACC 
ATTGCTACCA AATCCATTGA GGAATACCTC AAGTTGGTGG GACAACAGTA TCTTCATGAC 
GCATTGGGGG AGTTCATCAA AGCTTTGTAC GAGTCAGATG AGAACTGTGA AGTGGATCCC 
AGCAAGTGTT CCTCTAGTGA ACTGATAGAC CATCAGAGCA ACCTGAAGAT GTGCTGTGAG 
CTGGCTTTCT GCAAGATCAT CAACTCTTAC TGTGTTTTCC CTCGTGAGTT GAAAGAAGTG 
TTTGCATCGT GGAAGCAGCA GTGCCTGAAC CGTGGCAAAC AGGACATCAG CGAGCGGCTC 
ATCAGTGCCT CATTGTTTCT CCGTTTCCTG TGCCCTGCCA TCATGTCTCC CAGTCTCTTC 
AACCTTATGC AAGAGTACCC TGATGACCGT ACATCTCGCA CCCTGACTCT GATTGCCAAG 
GTCATTCAGA ACCTGGCTAA CTTTGCCAAG TTTGGTAACA AAGAAGAATA TATGGCATTC 
ATGAATGATT TTTTAGAACA TGAATGGGGT GGAATGAAGC GCTTTCTTTT GGAAATCTCT 
AATCCGGACA CCATCTCAAA CACCCCAGGC TTTGATGGTT ACATTGATCT GGGCCGAGAG 
CTTTCAGTCT TGCATTCCTT ACTATGGGAA GTAGTTTCCC AGCTTGATAA GGGTGAAAAT 
TCCTTCCTAC AGGCGACCGT GGCAAAATTG GGACCGCTCC CTCGAGTTCT TGCTGATATT 
ACCAAGTCTC TAACTAATCC TACACCAATA CAACAGCAGC TGAGACGCTT CACTGAGCAT 
AACTCCAGTC CAAATGTCAG TGGAAGCCTC TCCTCTGGGC TGCAGAAAAT ATTTGAAGAC 
CCCACTGACA GTGATTTGCA TAAACTAAAA TCTCCAAGCC AGGACAACAC AGATGGCTAT 
TTCAGAGGGA AAACGTTATT GCTGGTTCAG CAGGCCTCCT CCCAGAGCAT GACCTATTCT 
GAAAAGGATG AAAAGGAAAG TAGCCTTCCT AACGGTCGGA GCATCTCCCT CATGGACCTT 
CAAGACACTC ACGCTGCTCA AGTAGAGCAT GCATCTATCA TGCTCGATGT GCCTATGCGC 
TTAACTGGAA GCCAGCTTTC CATAACCCAG GTGGCCAGTA TCAAACAACT GCGGGAAACC 
CAGAGCACTC CCCAGAGTGC ACCCCAAGTG CGAAGGCCCT TGCACCCAGC CTTGAACCAG 
CCAGGCAGCC TCCAGCCCCT GTCATTCCAG AACCCTGTCT ATCACCTCAA TAACCCAATC 
CCAGCAATGC CAAAGGCCTC TGTGGATTCT AGTTTGGAGA ATCTAAGCAC TGCCAGTTCC 
AGAAGCCAAA GTAACAGTGA AGATTTCAAA CTCAGTGGGC CCAGCAATAG CAGCATGGAA 
GATTTCACTA AACGTAGCAC GCAGAGCGAG GATTTCTCCA GGCGGCACAC TGTACCAGAC 
AGACACATAC CTCTTGCTCT GCCGCGGCAG AATAGTACCG GGCAGGCCCA GATCCGGAAA 
ATGGACCAGG CTGGGTTAGG TGCCCGAGCC AAAGCCCCGC CGTCCTTGCC ACACAGTGCT 
TCCTTACGTA GCACGGGGAG CATGTCGGTG GCCTCTGCGG CCCTGGTGGC TGAACCTGTG 
CAGAATGGGA GCCGGTCCCG GCAGCAGTCC TCTTCCTCCA GAGAGAGCCC CGTTCCCAAA 
GTGAGAGCGA TCCAGAGACA ACAGACACAG CAGGTGCAGT CACCTGTGGA CTCTGCCACC 
ATGTCCCCAG TAGAGAGGAC AGCAGCCTGG GTGTTGAACA ATGGACAGTA TGAAGAGGAT 
GTGGAAGAGA CTGAGCAAAA TCCAGATGAA TCCAAGCATG CTGAGAAGTA TGAGCAGGAA 
ATTACTAAAC TGAAGGAGCG CCTGAGAGTG TCCAGCCGGC GCCTTGAGGA ATATGAGCGC 
CGCTTGCTGG TGCAGGAGCA GCAGATGCAG AAGCTGCTGC TGGAATACAA GGCCAGGCTG 
GAGGACAGCG AGGAGCGCCT GCGCAGGCAG CAGGAGGAGA AGGACAGCCA GATGAAAAGC 
ATCATCAGCA GGCTGATGGC TGTGGAAGAG GAACTGAAGA AGGATCATGC TGAGATGCAA 
GCAGTTATTG ATGCAAAGCA AAAAATAATC GATGCACAGG AAAAACGAAT CGTGTCCCTG 
GATTCGGCCA ACACCAGACT GATGAGCGCA CTGACCCAAG TGAAGGAGCG GTACAGCATG 
CAGGTCCGCA ATGGCATCTC CCCCACCAAC CCCACCAAGC TTTCCATCAC GGAGAATGGT 
GAATTCAAAA ACAGCAGCTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_003434980.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_003435028.2 Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OCe09202
Clone ID Related Accession (Same CDS sequence)	XM_003434980.3 , XM_003434980.4
Accession Version	XM_003434980.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3843bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	ras GTPase-activating protein nGAP isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_003434980.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTCT CCCCGTCGTC CGGAGGAGCC GCCGAGGCGC TGTCCTGGCC GGACATGTTC 
CCGGCGCTGG AGTCCGACTC GCCGATACCG CCGGAGGAGC TGGAAGCGGT TCTCCCCGTC 
GGCGGGGCCG TGGCCGGGGG CATGCTGGAT CGGATCCTTC TGGAGTCGGT GTGCCAGCAG 
CAGAGCTGGG TCCGGGTGTA CGATGTGAAG GGGCCACCTA CCCATCGTCT GTCTTGTGGA 
CAGTCGCCCT ACACAGAGAC GACAACATGG GAGCGGAAGT ATTGCATCCT CACCGACAGC 
CAGTTGGTGT TGCTCAACAA GGAGAAGGAG ATACCTGTGG AGGGAGGACA GGAGCAGCAG 
ACAGATTCCA CCAAAGGGCG ATGCCTGAGG AGAACCGTCA GTGTCCCTTC CGAGGGCCAG 
TTTCCTGAGT ACCCACCAGA GGGCGCCACC AAACTGGAGG TGCCGGCAGA AAGGTCCCCA 
CGCAGACGGA GTATCTCAGG GACCAGTACA TCAGAGAAAC CCAACTCCAT GGACACTGTA 
AATACCTCAC CCTTCAAAGT ACCAGGGTTC TTCAGCAAGC GCCTGAAAGG CTCCATCAAG 
AGGACCAAAA GCCAATCAAA GCTTGACAGA AACACGAGCT TTCGGCTACC ATCCCTTCGT 
AATGCAGATG ACAGGACTCG CGGTCTGCCT AAACTAAAAG AGTCACGTTC CCATGAGTCA 
TTGCTGAGCC CTTGTAGTGC AGTGGAATGT CTGGATCTTG GCAGAGGAGA GCCTGTGTCA 
GTGAAGCCAC TCCATAGCAG CATCCTTGGA CAAGACTTCT GCTTTGAGGT TACGTACTTA 
AGTGGAAGTA AATGCTTCAG CTGTAACTCT GCTTCAGAGA GAGATAAGTG GATGGAGAAC 
CTTCGTAGGA CAGTTCAACC AAATAAGGAC AATTGCAGAC GAGCTGAAAA CGTTCTTCGT 
TTATGGATCA TTGAAGCCAA GGACCTTGCC CCCAAAAAGA AATATTTCTG TGAACTGTGC 
CTTGATGATA CCCTCTTTGC TCGTACAACC AGCAAGACCA AAGCAGATAA TATTTTCTGG 
GGTGAACATT TTGAATTTTA CAGCCTTCCA CCTCTTCATA GCATCACAGT TCACATTTAT 
AAAGATGTGG AAAAAAAGAA AAAAAAAGAC AAGAATAATT ATGTAGGGCT AGTCAACATT 
CCCACTGCCA GTGTGACTGG GCGCCAATTT GTAGAAAAGT GGTATCCAGT GAGTACCCCT 
ACACCCAACA AAGGAAAGAC CGGAGGACCT TCTATTCGGA TTAAATCACG TTTCCAAACT 
ATCACCATTC TGCCTATGGA GCAATACAAA GAATTTGCAG AATTTGTCAC CAGCAACTAT 
ACCATGCTGT GTTCTGTCCT TGAACCAGTA ATTAGTGTGA GAAATAAAGA GGAGTTGGCC 
TGTGCCCTAG TGCACATTCT TCAAAGTACT GGCAGAGCCA AGGATTTTCT GACCGACTTG 
GTGATGTCTG AGGTGGATCG CTGTGGAGAG CATGACGTCT TGATCTTCAG AGAGAACACC 
ATTGCTACCA AATCCATTGA GGAATACCTC AAGTTGGTGG GACAACAGTA TCTTCATGAC 
GCATTGGGGG AGTTCATCAA AGCTTTGTAC GAGTCAGATG AGAACTGTGA AGTGGATCCC 
AGCAAGTGTT CCTCTAGTGA ACTGATAGAC CATCAGAGCA ACCTGAAGAT GTGCTGTGAG 
CTGGCTTTCT GCAAGATCAT CAACTCTTAC TGTGTTTTCC CTCGTGAGTT GAAAGAAGTG 
TTTGCATCGT GGAAGCAGCA GTGCCTGAAC CGTGGCAAAC AGGACATCAG CGAGCGGCTC 
ATCAGTGCCT CATTGTTTCT CCGTTTCCTG TGCCCTGCCA TCATGTCTCC CAGTCTCTTC 
AACCTTATGC AAGAGTACCC TGATGACCGT ACATCTCGCA CCCTGACTCT GATTGCCAAG 
GTCATTCAGA ACCTGGCTAA CTTTGCCAAG TTTGGTAACA AAGAAGAATA TATGGCATTC 
ATGAATGATT TTTTAGAACA TGAATGGGGT GGAATGAAGC GCTTTCTTTT GGAAATCTCT 
AATCCGGACA CCATCTCAAA CACCCCAGGC TTTGATGGTT ACATTGATCT GGGCCGAGAG 
CTTTCAGTCT TGCATTCCTT ACTATGGGAA GTAGTTTCCC AGCTTGATAA GGCGACCGTG 
GCAAAATTGG GACCGCTCCC TCGAGTTCTT GCTGATATTA CCAAGTCTCT AACTAATCCT 
ACACCAATAC AACAGCAGCT GAGACGCTTC ACTGAGCATA ACTCCAGTCC AAATGTCAGT 
GGAAGCCTCT CCTCTGGGCT GCAGAAAATA TTTGAAGACC CCACTGACAG TGATTTGCAT 
AAACTAAAAT CTCCAAGCCA GGACAACACA GATGGCTATT TCAGAGGGAA AACGTTATTG 
CTGGTTCAGC AGGCCTCCTC CCAGAGCATG ACCTATTCTG AAAAGGATGA AAAGGAAAGT 
AGCCTTCCTA ACGGTCGGAG CATCTCCCTC ATGGACCTTC AAGACACTCA CGCTGCTCAA 
GTAGAGCATG CATCTATCAT GCTCGATGTG CCTATGCGCT TAACTGGAAG CCAGCTTTCC 
ATAACCCAGG TGGCCAGTAT CAAACAACTG CGGGAAACCC AGAGCACTCC CCAGAGTGCA 
CCCCAAGTGC GAAGGCCCTT GCACCCAGCC TTGAACCAGC CAGGCAGCCT CCAGCCCCTG 
TCATTCCAGA ACCCTGTCTA TCACCTCAAT AACCCAATCC CAGCAATGCC AAAGGCCTCT 
GTGGATTCTA GTTTGGAGAA TCTAAGCACT GCCAGTTCCA GAAGCCAAAG TAACAGTGAA 
GATTTCAAAC TCAGTGGGCC CAGCAATAGC AGCATGGAAG ATTTCACTAA ACGTAGCACG 
CAGAGCGAGG ATTTCTCCAG GCGGCACACT GTACCAGACA GACACATACC TCTTGCTCTG 
CCGCGGCAGA ATAGTACCGG GCAGGCCCAG ATCCGGAAAA TGGACCAGGC TGGGTTAGGT 
GCCCGAGCCA AAGCCCCGCC GTCCTTGCCA CACAGTGCTT CCTTACGTAG CACGGGGAGC 
ATGTCGGTGG CCTCTGCGGC CCTGGTGGCT GAACCTGTGC AGAATGGGAG CCGGTCCCGG 
CAGCAGTCCT CTTCCTCCAG AGAGAGCCCC GTTCCCAAAG TGAGAGCGAT CCAGAGACAA 
CAGACACAGC AGGTGCAGTC ACCTGTGGAC TCTGCCACCA TGTCCCCAGT AGAGAGGACA 
GCAGCCTGGG TGTTGAACAA TGGACAGTAT GAAGAGGATG TGGAAGAGAC TGAGCAAAAT 
CCAGATGAAT CCAAGCATGC TGAGAAGTAT GAGCAGGAAA TTACTAAACT GAAGGAGCGC 
CTGAGAGTGT CCAGCCGGCG CCTTGAGGAA TATGAGCGCC GCTTGCTGGT GCAGGAGCAG 
CAGATGCAGA AGCTGCTGCT GGAATACAAG GCCAGGCTGG AGGACAGCGA GGAGCGCCTG 
CGCAGGCAGC AGGAGGAGAA GGACAGCCAG ATGAAAAGCA TCATCAGCAG GCTGATGGCT 
GTGGAAGAGG AACTGAAGAA GGATCATGCT GAGATGCAAG CAGTTATTGA TGCAAAGCAA 
AAAATAATCG ATGCACAGGA AAAACGAATC GTGTCCCTGG ATTCGGCCAA CACCAGACTG 
ATGAGCGCAC TGACCCAAGT GAAGGAGCGG TACAGCATGC AGGTCCGCAA TGGCATCTCC 
CCCACCAACC CCACCAAGCT TTCCATCACG GAGAATGGTG AATTCAAAAA CAGCAGCTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003435028.2
CDS	54..3896
Translation	MELSPSSGGAAEALSWPDMFPALESDSPIPPEELEAVLPVGGAVAGGMLDRILLESVCQQQSWVRVYDVKGPPTHRLSCGQSPYTETTTWERKYCILTDSQLVLLNKEKEIPVEGGQEQQTDSTKGRCLRRTVSVPSEGQFPEYPPEGATKLEVPAERSPRRRSISGTSTSEKPNSMDTVNTSPFKVPGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNADDRTRGLPKLKESRSHESLLSPCSAVECLDLGRGEPVSVKPLHSSILGQDFCFEVTYLSGSKCFSCNSASERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYSLPPLHSITVHIYKDVEKKKKKDKNNYVGLVNIPTASVTGRQFVEKWYPVSTPTPNKGKTGGPSIRIKSRFQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEELACALVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTIATKSIEEYLKLVGQQYLHDALGEFIKALYESDENCEVDPSKCSSSELIDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCLNRGKQDISERLISASLFLRFLCPAIMSPSLFNLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNTPGFDGYIDLGRELSVLHSLLWEVVSQLDKATVAKLGPLPRVLADITKSLTNPTPIQQQLRRFTEHNSSPNVSGSLSSGLQKIFEDPTDSDLHKLKSPSQDNTDGYFRGKTLLLVQQASSQSMTYSEKDEKESSLPNGRSISLMDLQDTHAAQVEHASIMLDVPMRLTGSQLSITQVASIKQLRETQSTPQSAPQVRRPLHPALNQPGSLQPLSFQNPVYHLNNPIPAMPKASVDSSLENLSTASSRSQSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTVPDRHIPLALPRQNSTGQAQIRKMDQAGLGARAKAPPSLPHSASLRSTGSMSVASAALVAEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEDVEETEQNPDESKHAEKYEQEITKLKERLRVSSRRLEEYERRLLVQEQQMQKLLLEYKARLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQEKRIVSLDSANTRLMSALTQVKERYSMQVRNGISPTNPTKLSITENGEFKNSSC

Target ORF information:

RefSeq Version	XM_003434980.3
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003434980.3

ORF Insert Sequence:

ATGGAGCTCT CCCCGTCGTC CGGAGGAGCC GCCGAGGCGC TGTCCTGGCC GGACATGTTC 
CCGGCGCTGG AGTCCGACTC GCCGATACCG CCGGAGGAGC TGGAAGCGGT TCTCCCCGTC 
GGCGGGGCCG TGGCCGGGGG CATGCTGGAT CGGATCCTTC TGGAGTCGGT GTGCCAGCAG 
CAGAGCTGGG TCCGGGTGTA CGATGTGAAG GGGCCACCTA CCCATCGTCT GTCTTGTGGA 
CAGTCGCCCT ACACAGAGAC GACAACATGG GAGCGGAAGT ATTGCATCCT CACCGACAGC 
CAGTTGGTGT TGCTCAACAA GGAGAAGGAG ATACCTGTGG AGGGAGGACA GGAGCAGCAG 
ACAGATTCCA CCAAAGGGCG ATGCCTGAGG AGAACCGTCA GTGTCCCTTC CGAGGGCCAG 
TTTCCTGAGT ACCCACCAGA GGGCGCCACC AAACTGGAGG TGCCGGCAGA AAGGTCCCCA 
CGCAGACGGA GTATCTCAGG GACCAGTACA TCAGAGAAAC CCAACTCCAT GGACACTGTA 
AATACCTCAC CCTTCAAAGT ACCAGGGTTC TTCAGCAAGC GCCTGAAAGG CTCCATCAAG 
AGGACCAAAA GCCAATCAAA GCTTGACAGA AACACGAGCT TTCGGCTACC ATCCCTTCGT 
AATGCAGATG ACAGGACTCG CGGTCTGCCT AAACTAAAAG AGTCACGTTC CCATGAGTCA 
TTGCTGAGCC CTTGTAGTGC AGTGGAATGT CTGGATCTTG GCAGAGGAGA GCCTGTGTCA 
GTGAAGCCAC TCCATAGCAG CATCCTTGGA CAAGACTTCT GCTTTGAGGT TACGTACTTA 
AGTGGAAGTA AATGCTTCAG CTGTAACTCT GCTTCAGAGA GAGATAAGTG GATGGAGAAC 
CTTCGTAGGA CAGTTCAACC AAATAAGGAC AATTGCAGAC GAGCTGAAAA CGTTCTTCGT 
TTATGGATCA TTGAAGCCAA GGACCTTGCC CCCAAAAAGA AATATTTCTG TGAACTGTGC 
CTTGATGATA CCCTCTTTGC TCGTACAACC AGCAAGACCA AAGCAGATAA TATTTTCTGG 
GGTGAACATT TTGAATTTTA CAGCCTTCCA CCTCTTCATA GCATCACAGT TCACATTTAT 
AAAGATGTGG AAAAAAAGAA AAAAAAAGAC AAGAATAATT ATGTAGGGCT AGTCAACATT 
CCCACTGCCA GTGTGACTGG GCGCCAATTT GTAGAAAAGT GGTATCCAGT GAGTACCCCT 
ACACCCAACA AAGGAAAGAC CGGAGGACCT TCTATTCGGA TTAAATCACG TTTCCAAACT 
ATCACCATTC TGCCTATGGA GCAATACAAA GAATTTGCAG AATTTGTCAC CAGCAACTAT 
ACCATGCTGT GTTCTGTCCT TGAACCAGTA ATTAGTGTGA GAAATAAAGA GGAGTTGGCC 
TGTGCCCTAG TGCACATTCT TCAAAGTACT GGCAGAGCCA AGGATTTTCT GACCGACTTG 
GTGATGTCTG AGGTGGATCG CTGTGGAGAG CATGACGTCT TGATCTTCAG AGAGAACACC 
ATTGCTACCA AATCCATTGA GGAATACCTC AAGTTGGTGG GACAACAGTA TCTTCATGAC 
GCATTGGGGG AGTTCATCAA AGCTTTGTAC GAGTCAGATG AGAACTGTGA AGTGGATCCC 
AGCAAGTGTT CCTCTAGTGA ACTGATAGAC CATCAGAGCA ACCTGAAGAT GTGCTGTGAG 
CTGGCTTTCT GCAAGATCAT CAACTCTTAC TGTGTTTTCC CTCGTGAGTT GAAAGAAGTG 
TTTGCATCGT GGAAGCAGCA GTGCCTGAAC CGTGGCAAAC AGGACATCAG CGAGCGGCTC 
ATCAGTGCCT CATTGTTTCT CCGTTTCCTG TGCCCTGCCA TCATGTCTCC CAGTCTCTTC 
AACCTTATGC AAGAGTACCC TGATGACCGT ACATCTCGCA CCCTGACTCT GATTGCCAAG 
GTCATTCAGA ACCTGGCTAA CTTTGCCAAG TTTGGTAACA AAGAAGAATA TATGGCATTC 
ATGAATGATT TTTTAGAACA TGAATGGGGT GGAATGAAGC GCTTTCTTTT GGAAATCTCT 
AATCCGGACA CCATCTCAAA CACCCCAGGC TTTGATGGTT ACATTGATCT GGGCCGAGAG 
CTTTCAGTCT TGCATTCCTT ACTATGGGAA GTAGTTTCCC AGCTTGATAA GGCGACCGTG 
GCAAAATTGG GACCGCTCCC TCGAGTTCTT GCTGATATTA CCAAGTCTCT AACTAATCCT 
ACACCAATAC AACAGCAGCT GAGACGCTTC ACTGAGCATA ACTCCAGTCC AAATGTCAGT 
GGAAGCCTCT CCTCTGGGCT GCAGAAAATA TTTGAAGACC CCACTGACAG TGATTTGCAT 
AAACTAAAAT CTCCAAGCCA GGACAACACA GATGGCTATT TCAGAGGGAA AACGTTATTG 
CTGGTTCAGC AGGCCTCCTC CCAGAGCATG ACCTATTCTG AAAAGGATGA AAAGGAAAGT 
AGCCTTCCTA ACGGTCGGAG CATCTCCCTC ATGGACCTTC AAGACACTCA CGCTGCTCAA 
GTAGAGCATG CATCTATCAT GCTCGATGTG CCTATGCGCT TAACTGGAAG CCAGCTTTCC 
ATAACCCAGG TGGCCAGTAT CAAACAACTG CGGGAAACCC AGAGCACTCC CCAGAGTGCA 
CCCCAAGTGC GAAGGCCCTT GCACCCAGCC TTGAACCAGC CAGGCAGCCT CCAGCCCCTG 
TCATTCCAGA ACCCTGTCTA TCACCTCAAT AACCCAATCC CAGCAATGCC AAAGGCCTCT 
GTGGATTCTA GTTTGGAGAA TCTAAGCACT GCCAGTTCCA GAAGCCAAAG TAACAGTGAA 
GATTTCAAAC TCAGTGGGCC CAGCAATAGC AGCATGGAAG ATTTCACTAA ACGTAGCACG 
CAGAGCGAGG ATTTCTCCAG GCGGCACACT GTACCAGACA GACACATACC TCTTGCTCTG 
CCGCGGCAGA ATAGTACCGG GCAGGCCCAG ATCCGGAAAA TGGACCAGGC TGGGTTAGGT 
GCCCGAGCCA AAGCCCCGCC GTCCTTGCCA CACAGTGCTT CCTTACGTAG CACGGGGAGC 
ATGTCGGTGG CCTCTGCGGC CCTGGTGGCT GAACCTGTGC AGAATGGGAG CCGGTCCCGG 
CAGCAGTCCT CTTCCTCCAG AGAGAGCCCC GTTCCCAAAG TGAGAGCGAT CCAGAGACAA 
CAGACACAGC AGGTGCAGTC ACCTGTGGAC TCTGCCACCA TGTCCCCAGT AGAGAGGACA 
GCAGCCTGGG TGTTGAACAA TGGACAGTAT GAAGAGGATG TGGAAGAGAC TGAGCAAAAT 
CCAGATGAAT CCAAGCATGC TGAGAAGTAT GAGCAGGAAA TTACTAAACT GAAGGAGCGC 
CTGAGAGTGT CCAGCCGGCG CCTTGAGGAA TATGAGCGCC GCTTGCTGGT GCAGGAGCAG 
CAGATGCAGA AGCTGCTGCT GGAATACAAG GCCAGGCTGG AGGACAGCGA GGAGCGCCTG 
CGCAGGCAGC AGGAGGAGAA GGACAGCCAG ATGAAAAGCA TCATCAGCAG GCTGATGGCT 
GTGGAAGAGG AACTGAAGAA GGATCATGCT GAGATGCAAG CAGTTATTGA TGCAAAGCAA 
AAAATAATCG ATGCACAGGA AAAACGAATC GTGTCCCTGG ATTCGGCCAA CACCAGACTG 
ATGAGCGCAC TGACCCAAGT GAAGGAGCGG TACAGCATGC AGGTCCGCAA TGGCATCTCC 
CCCACCAACC CCACCAAGCT TTCCATCACG GAGAATGGTG AATTCAAAAA CAGCAGCTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_003434980.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_003435028.2 Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X7, mRNA.

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CloneID	OCe09202
Clone ID Related Accession (Same CDS sequence)	XM_003434980.3 , XM_003434980.4
Accession Version	XM_003434980.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3843bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	ras GTPase-activating protein nGAP isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_003434980.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTCT CCCCGTCGTC CGGAGGAGCC GCCGAGGCGC TGTCCTGGCC GGACATGTTC 
CCGGCGCTGG AGTCCGACTC GCCGATACCG CCGGAGGAGC TGGAAGCGGT TCTCCCCGTC 
GGCGGGGCCG TGGCCGGGGG CATGCTGGAT CGGATCCTTC TGGAGTCGGT GTGCCAGCAG 
CAGAGCTGGG TCCGGGTGTA CGATGTGAAG GGGCCACCTA CCCATCGTCT GTCTTGTGGA 
CAGTCGCCCT ACACAGAGAC GACAACATGG GAGCGGAAGT ATTGCATCCT CACCGACAGC 
CAGTTGGTGT TGCTCAACAA GGAGAAGGAG ATACCTGTGG AGGGAGGACA GGAGCAGCAG 
ACAGATTCCA CCAAAGGGCG ATGCCTGAGG AGAACCGTCA GTGTCCCTTC CGAGGGCCAG 
TTTCCTGAGT ACCCACCAGA GGGCGCCACC AAACTGGAGG TGCCGGCAGA AAGGTCCCCA 
CGCAGACGGA GTATCTCAGG GACCAGTACA TCAGAGAAAC CCAACTCCAT GGACACTGTA 
AATACCTCAC CCTTCAAAGT ACCAGGGTTC TTCAGCAAGC GCCTGAAAGG CTCCATCAAG 
AGGACCAAAA GCCAATCAAA GCTTGACAGA AACACGAGCT TTCGGCTACC ATCCCTTCGT 
AATGCAGATG ACAGGACTCG CGGTCTGCCT AAACTAAAAG AGTCACGTTC CCATGAGTCA 
TTGCTGAGCC CTTGTAGTGC AGTGGAATGT CTGGATCTTG GCAGAGGAGA GCCTGTGTCA 
GTGAAGCCAC TCCATAGCAG CATCCTTGGA CAAGACTTCT GCTTTGAGGT TACGTACTTA 
AGTGGAAGTA AATGCTTCAG CTGTAACTCT GCTTCAGAGA GAGATAAGTG GATGGAGAAC 
CTTCGTAGGA CAGTTCAACC AAATAAGGAC AATTGCAGAC GAGCTGAAAA CGTTCTTCGT 
TTATGGATCA TTGAAGCCAA GGACCTTGCC CCCAAAAAGA AATATTTCTG TGAACTGTGC 
CTTGATGATA CCCTCTTTGC TCGTACAACC AGCAAGACCA AAGCAGATAA TATTTTCTGG 
GGTGAACATT TTGAATTTTA CAGCCTTCCA CCTCTTCATA GCATCACAGT TCACATTTAT 
AAAGATGTGG AAAAAAAGAA AAAAAAAGAC AAGAATAATT ATGTAGGGCT AGTCAACATT 
CCCACTGCCA GTGTGACTGG GCGCCAATTT GTAGAAAAGT GGTATCCAGT GAGTACCCCT 
ACACCCAACA AAGGAAAGAC CGGAGGACCT TCTATTCGGA TTAAATCACG TTTCCAAACT 
ATCACCATTC TGCCTATGGA GCAATACAAA GAATTTGCAG AATTTGTCAC CAGCAACTAT 
ACCATGCTGT GTTCTGTCCT TGAACCAGTA ATTAGTGTGA GAAATAAAGA GGAGTTGGCC 
TGTGCCCTAG TGCACATTCT TCAAAGTACT GGCAGAGCCA AGGATTTTCT GACCGACTTG 
GTGATGTCTG AGGTGGATCG CTGTGGAGAG CATGACGTCT TGATCTTCAG AGAGAACACC 
ATTGCTACCA AATCCATTGA GGAATACCTC AAGTTGGTGG GACAACAGTA TCTTCATGAC 
GCATTGGGGG AGTTCATCAA AGCTTTGTAC GAGTCAGATG AGAACTGTGA AGTGGATCCC 
AGCAAGTGTT CCTCTAGTGA ACTGATAGAC CATCAGAGCA ACCTGAAGAT GTGCTGTGAG 
CTGGCTTTCT GCAAGATCAT CAACTCTTAC TGTGTTTTCC CTCGTGAGTT GAAAGAAGTG 
TTTGCATCGT GGAAGCAGCA GTGCCTGAAC CGTGGCAAAC AGGACATCAG CGAGCGGCTC 
ATCAGTGCCT CATTGTTTCT CCGTTTCCTG TGCCCTGCCA TCATGTCTCC CAGTCTCTTC 
AACCTTATGC AAGAGTACCC TGATGACCGT ACATCTCGCA CCCTGACTCT GATTGCCAAG 
GTCATTCAGA ACCTGGCTAA CTTTGCCAAG TTTGGTAACA AAGAAGAATA TATGGCATTC 
ATGAATGATT TTTTAGAACA TGAATGGGGT GGAATGAAGC GCTTTCTTTT GGAAATCTCT 
AATCCGGACA CCATCTCAAA CACCCCAGGC TTTGATGGTT ACATTGATCT GGGCCGAGAG 
CTTTCAGTCT TGCATTCCTT ACTATGGGAA GTAGTTTCCC AGCTTGATAA GGCGACCGTG 
GCAAAATTGG GACCGCTCCC TCGAGTTCTT GCTGATATTA CCAAGTCTCT AACTAATCCT 
ACACCAATAC AACAGCAGCT GAGACGCTTC ACTGAGCATA ACTCCAGTCC AAATGTCAGT 
GGAAGCCTCT CCTCTGGGCT GCAGAAAATA TTTGAAGACC CCACTGACAG TGATTTGCAT 
AAACTAAAAT CTCCAAGCCA GGACAACACA GATGGCTATT TCAGAGGGAA AACGTTATTG 
CTGGTTCAGC AGGCCTCCTC CCAGAGCATG ACCTATTCTG AAAAGGATGA AAAGGAAAGT 
AGCCTTCCTA ACGGTCGGAG CATCTCCCTC ATGGACCTTC AAGACACTCA CGCTGCTCAA 
GTAGAGCATG CATCTATCAT GCTCGATGTG CCTATGCGCT TAACTGGAAG CCAGCTTTCC 
ATAACCCAGG TGGCCAGTAT CAAACAACTG CGGGAAACCC AGAGCACTCC CCAGAGTGCA 
CCCCAAGTGC GAAGGCCCTT GCACCCAGCC TTGAACCAGC CAGGCAGCCT CCAGCCCCTG 
TCATTCCAGA ACCCTGTCTA TCACCTCAAT AACCCAATCC CAGCAATGCC AAAGGCCTCT 
GTGGATTCTA GTTTGGAGAA TCTAAGCACT GCCAGTTCCA GAAGCCAAAG TAACAGTGAA 
GATTTCAAAC TCAGTGGGCC CAGCAATAGC AGCATGGAAG ATTTCACTAA ACGTAGCACG 
CAGAGCGAGG ATTTCTCCAG GCGGCACACT GTACCAGACA GACACATACC TCTTGCTCTG 
CCGCGGCAGA ATAGTACCGG GCAGGCCCAG ATCCGGAAAA TGGACCAGGC TGGGTTAGGT 
GCCCGAGCCA AAGCCCCGCC GTCCTTGCCA CACAGTGCTT CCTTACGTAG CACGGGGAGC 
ATGTCGGTGG CCTCTGCGGC CCTGGTGGCT GAACCTGTGC AGAATGGGAG CCGGTCCCGG 
CAGCAGTCCT CTTCCTCCAG AGAGAGCCCC GTTCCCAAAG TGAGAGCGAT CCAGAGACAA 
CAGACACAGC AGGTGCAGTC ACCTGTGGAC TCTGCCACCA TGTCCCCAGT AGAGAGGACA 
GCAGCCTGGG TGTTGAACAA TGGACAGTAT GAAGAGGATG TGGAAGAGAC TGAGCAAAAT 
CCAGATGAAT CCAAGCATGC TGAGAAGTAT GAGCAGGAAA TTACTAAACT GAAGGAGCGC 
CTGAGAGTGT CCAGCCGGCG CCTTGAGGAA TATGAGCGCC GCTTGCTGGT GCAGGAGCAG 
CAGATGCAGA AGCTGCTGCT GGAATACAAG GCCAGGCTGG AGGACAGCGA GGAGCGCCTG 
CGCAGGCAGC AGGAGGAGAA GGACAGCCAG ATGAAAAGCA TCATCAGCAG GCTGATGGCT 
GTGGAAGAGG AACTGAAGAA GGATCATGCT GAGATGCAAG CAGTTATTGA TGCAAAGCAA 
AAAATAATCG ATGCACAGGA AAAACGAATC GTGTCCCTGG ATTCGGCCAA CACCAGACTG 
ATGAGCGCAC TGACCCAAGT GAAGGAGCGG TACAGCATGC AGGTCCGCAA TGGCATCTCC 
CCCACCAACC CCACCAAGCT TTCCATCACG GAGAATGGTG AATTCAAAAA CAGCAGCTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003435028.2
CDS	34..3876
Translation	MELSPSSGGAAEALSWPDMFPALESDSPIPPEELEAVLPVGGAVAGGMLDRILLESVCQQQSWVRVYDVKGPPTHRLSCGQSPYTETTTWERKYCILTDSQLVLLNKEKEIPVEGGQEQQTDSTKGRCLRRTVSVPSEGQFPEYPPEGATKLEVPAERSPRRRSISGTSTSEKPNSMDTVNTSPFKVPGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNADDRTRGLPKLKESRSHESLLSPCSAVECLDLGRGEPVSVKPLHSSILGQDFCFEVTYLSGSKCFSCNSASERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYSLPPLHSITVHIYKDVEKKKKKDKNNYVGLVNIPTASVTGRQFVEKWYPVSTPTPNKGKTGGPSIRIKSRFQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEELACALVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTIATKSIEEYLKLVGQQYLHDALGEFIKALYESDENCEVDPSKCSSSELIDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCLNRGKQDISERLISASLFLRFLCPAIMSPSLFNLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNTPGFDGYIDLGRELSVLHSLLWEVVSQLDKATVAKLGPLPRVLADITKSLTNPTPIQQQLRRFTEHNSSPNVSGSLSSGLQKIFEDPTDSDLHKLKSPSQDNTDGYFRGKTLLLVQQASSQSMTYSEKDEKESSLPNGRSISLMDLQDTHAAQVEHASIMLDVPMRLTGSQLSITQVASIKQLRETQSTPQSAPQVRRPLHPALNQPGSLQPLSFQNPVYHLNNPIPAMPKASVDSSLENLSTASSRSQSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTVPDRHIPLALPRQNSTGQAQIRKMDQAGLGARAKAPPSLPHSASLRSTGSMSVASAALVAEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEDVEETEQNPDESKHAEKYEQEITKLKERLRVSSRRLEEYERRLLVQEQQMQKLLLEYKARLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQEKRIVSLDSANTRLMSALTQVKERYSMQVRNGISPTNPTKLSITENGEFKNSSC

Target ORF information:

RefSeq Version	XM_003434980.4
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003434980.4

ORF Insert Sequence:

ATGGAGCTCT CCCCGTCGTC CGGAGGAGCC GCCGAGGCGC TGTCCTGGCC GGACATGTTC 
CCGGCGCTGG AGTCCGACTC GCCGATACCG CCGGAGGAGC TGGAAGCGGT TCTCCCCGTC 
GGCGGGGCCG TGGCCGGGGG CATGCTGGAT CGGATCCTTC TGGAGTCGGT GTGCCAGCAG 
CAGAGCTGGG TCCGGGTGTA CGATGTGAAG GGGCCACCTA CCCATCGTCT GTCTTGTGGA 
CAGTCGCCCT ACACAGAGAC GACAACATGG GAGCGGAAGT ATTGCATCCT CACCGACAGC 
CAGTTGGTGT TGCTCAACAA GGAGAAGGAG ATACCTGTGG AGGGAGGACA GGAGCAGCAG 
ACAGATTCCA CCAAAGGGCG ATGCCTGAGG AGAACCGTCA GTGTCCCTTC CGAGGGCCAG 
TTTCCTGAGT ACCCACCAGA GGGCGCCACC AAACTGGAGG TGCCGGCAGA AAGGTCCCCA 
CGCAGACGGA GTATCTCAGG GACCAGTACA TCAGAGAAAC CCAACTCCAT GGACACTGTA 
AATACCTCAC CCTTCAAAGT ACCAGGGTTC TTCAGCAAGC GCCTGAAAGG CTCCATCAAG 
AGGACCAAAA GCCAATCAAA GCTTGACAGA AACACGAGCT TTCGGCTACC ATCCCTTCGT 
AATGCAGATG ACAGGACTCG CGGTCTGCCT AAACTAAAAG AGTCACGTTC CCATGAGTCA 
TTGCTGAGCC CTTGTAGTGC AGTGGAATGT CTGGATCTTG GCAGAGGAGA GCCTGTGTCA 
GTGAAGCCAC TCCATAGCAG CATCCTTGGA CAAGACTTCT GCTTTGAGGT TACGTACTTA 
AGTGGAAGTA AATGCTTCAG CTGTAACTCT GCTTCAGAGA GAGATAAGTG GATGGAGAAC 
CTTCGTAGGA CAGTTCAACC AAATAAGGAC AATTGCAGAC GAGCTGAAAA CGTTCTTCGT 
TTATGGATCA TTGAAGCCAA GGACCTTGCC CCCAAAAAGA AATATTTCTG TGAACTGTGC 
CTTGATGATA CCCTCTTTGC TCGTACAACC AGCAAGACCA AAGCAGATAA TATTTTCTGG 
GGTGAACATT TTGAATTTTA CAGCCTTCCA CCTCTTCATA GCATCACAGT TCACATTTAT 
AAAGATGTGG AAAAAAAGAA AAAAAAAGAC AAGAATAATT ATGTAGGGCT AGTCAACATT 
CCCACTGCCA GTGTGACTGG GCGCCAATTT GTAGAAAAGT GGTATCCAGT GAGTACCCCT 
ACACCCAACA AAGGAAAGAC CGGAGGACCT TCTATTCGGA TTAAATCACG TTTCCAAACT 
ATCACCATTC TGCCTATGGA GCAATACAAA GAATTTGCAG AATTTGTCAC CAGCAACTAT 
ACCATGCTGT GTTCTGTCCT TGAACCAGTA ATTAGTGTGA GAAATAAAGA GGAGTTGGCC 
TGTGCCCTAG TGCACATTCT TCAAAGTACT GGCAGAGCCA AGGATTTTCT GACCGACTTG 
GTGATGTCTG AGGTGGATCG CTGTGGAGAG CATGACGTCT TGATCTTCAG AGAGAACACC 
ATTGCTACCA AATCCATTGA GGAATACCTC AAGTTGGTGG GACAACAGTA TCTTCATGAC 
GCATTGGGGG AGTTCATCAA AGCTTTGTAC GAGTCAGATG AGAACTGTGA AGTGGATCCC 
AGCAAGTGTT CCTCTAGTGA ACTGATAGAC CATCAGAGCA ACCTGAAGAT GTGCTGTGAG 
CTGGCTTTCT GCAAGATCAT CAACTCTTAC TGTGTTTTCC CTCGTGAGTT GAAAGAAGTG 
TTTGCATCGT GGAAGCAGCA GTGCCTGAAC CGTGGCAAAC AGGACATCAG CGAGCGGCTC 
ATCAGTGCCT CATTGTTTCT CCGTTTCCTG TGCCCTGCCA TCATGTCTCC CAGTCTCTTC 
AACCTTATGC AAGAGTACCC TGATGACCGT ACATCTCGCA CCCTGACTCT GATTGCCAAG 
GTCATTCAGA ACCTGGCTAA CTTTGCCAAG TTTGGTAACA AAGAAGAATA TATGGCATTC 
ATGAATGATT TTTTAGAACA TGAATGGGGT GGAATGAAGC GCTTTCTTTT GGAAATCTCT 
AATCCGGACA CCATCTCAAA CACCCCAGGC TTTGATGGTT ACATTGATCT GGGCCGAGAG 
CTTTCAGTCT TGCATTCCTT ACTATGGGAA GTAGTTTCCC AGCTTGATAA GGCGACCGTG 
GCAAAATTGG GACCGCTCCC TCGAGTTCTT GCTGATATTA CCAAGTCTCT AACTAATCCT 
ACACCAATAC AACAGCAGCT GAGACGCTTC ACTGAGCATA ACTCCAGTCC AAATGTCAGT 
GGAAGCCTCT CCTCTGGGCT GCAGAAAATA TTTGAAGACC CCACTGACAG TGATTTGCAT 
AAACTAAAAT CTCCAAGCCA GGACAACACA GATGGCTATT TCAGAGGGAA AACGTTATTG 
CTGGTTCAGC AGGCCTCCTC CCAGAGCATG ACCTATTCTG AAAAGGATGA AAAGGAAAGT 
AGCCTTCCTA ACGGTCGGAG CATCTCCCTC ATGGACCTTC AAGACACTCA CGCTGCTCAA 
GTAGAGCATG CATCTATCAT GCTCGATGTG CCTATGCGCT TAACTGGAAG CCAGCTTTCC 
ATAACCCAGG TGGCCAGTAT CAAACAACTG CGGGAAACCC AGAGCACTCC CCAGAGTGCA 
CCCCAAGTGC GAAGGCCCTT GCACCCAGCC TTGAACCAGC CAGGCAGCCT CCAGCCCCTG 
TCATTCCAGA ACCCTGTCTA TCACCTCAAT AACCCAATCC CAGCAATGCC AAAGGCCTCT 
GTGGATTCTA GTTTGGAGAA TCTAAGCACT GCCAGTTCCA GAAGCCAAAG TAACAGTGAA 
GATTTCAAAC TCAGTGGGCC CAGCAATAGC AGCATGGAAG ATTTCACTAA ACGTAGCACG 
CAGAGCGAGG ATTTCTCCAG GCGGCACACT GTACCAGACA GACACATACC TCTTGCTCTG 
CCGCGGCAGA ATAGTACCGG GCAGGCCCAG ATCCGGAAAA TGGACCAGGC TGGGTTAGGT 
GCCCGAGCCA AAGCCCCGCC GTCCTTGCCA CACAGTGCTT CCTTACGTAG CACGGGGAGC 
ATGTCGGTGG CCTCTGCGGC CCTGGTGGCT GAACCTGTGC AGAATGGGAG CCGGTCCCGG 
CAGCAGTCCT CTTCCTCCAG AGAGAGCCCC GTTCCCAAAG TGAGAGCGAT CCAGAGACAA 
CAGACACAGC AGGTGCAGTC ACCTGTGGAC TCTGCCACCA TGTCCCCAGT AGAGAGGACA 
GCAGCCTGGG TGTTGAACAA TGGACAGTAT GAAGAGGATG TGGAAGAGAC TGAGCAAAAT 
CCAGATGAAT CCAAGCATGC TGAGAAGTAT GAGCAGGAAA TTACTAAACT GAAGGAGCGC 
CTGAGAGTGT CCAGCCGGCG CCTTGAGGAA TATGAGCGCC GCTTGCTGGT GCAGGAGCAG 
CAGATGCAGA AGCTGCTGCT GGAATACAAG GCCAGGCTGG AGGACAGCGA GGAGCGCCTG 
CGCAGGCAGC AGGAGGAGAA GGACAGCCAG ATGAAAAGCA TCATCAGCAG GCTGATGGCT 
GTGGAAGAGG AACTGAAGAA GGATCATGCT GAGATGCAAG CAGTTATTGA TGCAAAGCAA 
AAAATAATCG ATGCACAGGA AAAACGAATC GTGTCCCTGG ATTCGGCCAA CACCAGACTG 
ATGAGCGCAC TGACCCAAGT GAAGGAGCGG TACAGCATGC AGGTCCGCAA TGGCATCTCC 
CCCACCAACC CCACCAAGCT TTCCATCACG GAGAATGGTG AATTCAAAAA CAGCAGCTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_005622478.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005622535.1 Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe09199
Clone ID Related Accession (Same CDS sequence)	XM_005622478.2 , XM_005622478.3
Accession Version	XM_005622478.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	ras GTPase-activating protein nGAP isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005622478.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTCGT GGAAGATGAG CATCTTTAAG AGACAGACAT CACCTTGTCC TCCAAGCTAT 
CGAAGATCTT TGTCAGAACC TGCTTATGCA AAATCAGAGA TAGGAATAAG CTTAAAGAGT 
TATCCATGGC GTGGTCCAGT GAAAATCAAG AGGAAGAAAA ATGAACATGC TGTTTCCAGT 
CTAGGAAAGC CACTGGGTGC TTATCGGTGG CAAACACATC CCAGCTGTAG GTGGAAAGGG 
GGCAAAAGGG AGAGGTTACA GACTAATAAA AGAGACTTGG ATGAACGGCA ACAAAAGGTC 
CTGCCAGAGA TGGTTGGTAG TAATGCTTCT TCCTCTGCTA TTGAGGTCCT TGGAGCACCA 
ACAGAGAAGC TGAACCTGGT CAACACCCGG TCACTTCGGG TTCCATTCAC CAGATCTATT 
TCAGAGCCAG AACCTCGCTG CAGTATCAGA TTTGTTAGAT CATGGCCGAC TACAGGAAGG 
TCAGAGGAGT CTGAACAGCA GGGATATTTC ATCCGTGGCA TTTTCTCTAC TCTTTCCAAT 
GGCTTCTCCA GGATGCTGAG TCTGAAAGGA GAATCAACCA ATGAGCCAGT AAGAGAAGAT 
GTGAAGGGGC CACCTACCCA TCGTCTGTCT TGTGGACAGT CGCCCTACAC AGAGACGACA 
ACATGGGAGC GGAAGTATTG CATCCTCACC GACAGCCAGT TGGTGTTGCT CAACAAGGAG 
AAGGAGATAC CTGTGGAGGG AGGACAGGAG CAGCAGACAG ATTCCACCAA AGGGCGATGC 
CTGAGGAGAA CCGTCAGTGT CCCTTCCGAG GGCCAGTTTC CTGAGTACCC ACCAGAGGGC 
GCCACCAAAC TGGAGGTGCC GGCAGAAAGG TCCCCACGCA GACGGAGTAT CTCAGGGACC 
AGTACATCAG AGAAACCCAA CTCCATGGAC ACTGTAAATA CCTCACCCTT CAAAGTACCA 
GGGTTCTTCA GCAAGCGCCT GAAAGGCTCC ATCAAGAGGA CCAAAAGCCA ATCAAAGCTT 
GACAGAAACA CGAGCTTTCG GCTACCATCC CTTCGTAATG CAGATGACAG GACTCGCGGT 
CTGCCTAAAC TAAAAGAGTC ACGTTCCCAT GAGTCATTGC TGAGCCCTTG TAGTGCAGTG 
GAATGTCTGG ATCTTGGCAG AGGAGAGCCT GTGTCAGTGA AGCCACTCCA TAGCAGCATC 
CTTGGACAAG ACTTCTGCTT TGAGGTTACG TACTTAAGTG GAAGTAAATG CTTCAGCTGT 
AACTCTGCTT CAGAGAGAGA TAAGTGGATG GAGAACCTTC GTAGGACAGT TCAACCAAAT 
AAGGACAATT GCAGACGAGC TGAAAACGTT CTTCGTTTAT GGATCATTGA AGCCAAGGAC 
CTTGCCCCCA AAAAGAAATA TTTCTGTGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT 
ACAACCAGCA AGACCAAAGC AGATAATATT TTCTGGGGTG AACATTTTGA ATTTTACAGC 
CTTCCACCTC TTCATAGCAT CACAGTTCAC ATTTATAAAG ATGTGGAAAA AAAGAAAAAA 
AAAGACAAGA ATAATTATGT AGGGCTAGTC AACATTCCCA CTGCCAGTGT GACTGGGCGC 
CAATTTGTAG AAAAGTGGTA TCCAGTGAGT ACCCCTACAC CCAACAAAGG AAAGACCGGA 
GGACCTTCTA TTCGGATTAA ATCACGTTTC CAAACTATCA CCATTCTGCC TATGGAGCAA 
TACAAAGAAT TTGCAGAATT TGTCACCAGC AACTATACCA TGCTGTGTTC TGTCCTTGAA 
CCAGTAATTA GTGTGAGAAA TAAAGAGGAG TTGGCCTGTG CCCTAGTGCA CATTCTTCAA 
AGTACTGGCA GAGCCAAGGA TTTTCTGACC GACTTGGTGA TGTCTGAGGT GGATCGCTGT 
GGAGAGCATG ACGTCTTGAT CTTCAGAGAG AACACCATTG CTACCAAATC CATTGAGGAA 
TACCTCAAGT TGGTGGGACA ACAGTATCTT CATGACGCAT TGGGGGAGTT CATCAAAGCT 
TTGTACGAGT CAGATGAGAA CTGTGAAGTG GATCCCAGCA AGTGTTCCTC TAGTGAACTG 
ATAGACCATC AGAGCAACCT GAAGATGTGC TGTGAGCTGG CTTTCTGCAA GATCATCAAC 
TCTTACTGTG TTTTCCCTCG TGAGTTGAAA GAAGTGTTTG CATCGTGGAA GCAGCAGTGC 
CTGAACCGTG GCAAACAGGA CATCAGCGAG CGGCTCATCA GTGCCTCATT GTTTCTCCGT 
TTCCTGTGCC CTGCCATCAT GTCTCCCAGT CTCTTCAACC TTATGCAAGA GTACCCTGAT 
GACCGTACAT CTCGCACCCT GACTCTGATT GCCAAGGTCA TTCAGAACCT GGCTAACTTT 
GCCAAGTTTG GTAACAAAGA AGAATATATG GCATTCATGA ATGATTTTTT AGAACATGAA 
TGGGGTGGAA TGAAGCGCTT TCTTTTGGAA ATCTCTAATC CGGACACCAT CTCAAACACC 
CCAGGCTTTG ATGGTTACAT TGATCTGGGC CGAGAGCTTT CAGTCTTGCA TTCCTTACTA 
TGGGAAGTAG TTTCCCAGCT TGATAAGGGT GAAAATTCCT TCCTACAGGC GACCGTGGCA 
AAATTGGGAC CGCTCCCTCG AGTTCTTGCT GATATTACCA AGTCTCTAAC TAATCCTACA 
CCAATACAAC AGCAGCTGAG ACGCTTCACT GAGCATAACT CCAGTCCAAA TGTCAGTGGA 
AGCCTCTCCT CTGGGCTGCA GAAAATATTT GAAGACCCCA CTGACAGTGA TTTGCATAAA 
CTAAAATCTC CAAGCCAGGA CAACACAGAT GGCTATTTCA GAGGGAAAAC GTTATTGCTG 
GTTCAGCAGG CCTCCTCCCA GAGCATGACC TATTCTGAAA AGGATGAAAA GGAAAGTAGC 
CTTCCTAACG GTCGGAGCAT CTCCCTCATG GACCTTCAAG ACACTCACGC TGCTCAAGTA 
GAGCATGCAT CTATCATGCT CGATGTGCCT ATGCGCTTAA CTGGAAGCCA GCTTTCCATA 
ACCCAGGTGG CCAGTATCAA ACAACTGCGG GAAACCCAGA GCACTCCCCA GAGTGCACCC 
CAAGTGCGAA GGCCCTTGCA CCCAGCCTTG AACCAGCCAG GCAGCCTCCA GCCCCTGTCA 
TTCCAGAACC CTGTCTATCA CCTCAATAAC CCAATCCCAG CAATGCCAAA GGCCTCTGTG 
GATTCTAGTT TGGAGAATCT AAGCACTGCC AGTTCCAGAA GCCAAAGTAA CAGTGAAGAT 
TTCAAACTCA GTGGGCCCAG CAATAGCAGC ATGGAAGATT TCACTAAACG TAGCACGCAG 
AGCGAGGATT TCTCCAGGCG GCACACTGTA CCAGACAGAC ACATACCTCT TGCTCTGCCG 
CGGCAGAATA GTACCGGGCA GGCCCAGATC CGGAAAATGG ACCAGGCTGG GTTAGGTGCC 
CGAGCCAAAG CCCCGCCGTC CTTGCCACAC AGTGCTTCCT TACGTAGCAC GGGGAGCATG 
TCGGTGGCCT CTGCGGCCCT GGTGGCTGAA CCTGTGCAGA ATGGGAGCCG GTCCCGGCAG 
CAGTCCTCTT CCTCCAGAGA GAGCCCCGTT CCCAAAGTGA GAGCGATCCA GAGACAACAG 
ACACAGCAGG TGCAGTCACC TGTGGACTCT GCCACCATGT CCCCAGTAGA GAGGACAGCA 
GCCTGGGTGT TGAACAATGG ACAGTATGAA GAGGATGTGG AAGAGACTGA GCAAAATCCA 
GATGAATCCA AGCATGCTGA GAAGTATGAG CAGGAAATTA CTAAACTGAA GGAGCGCCTG 
AGAGTGTCCA GCCGGCGCCT TGAGGAATAT GAGCGCCGCT TGCTGGTGCA GGAGCAGCAG 
ATGCAGAAGC TGCTGCTGGA ATACAAGGCC AGGCTGGAGG ACAGCGAGGA GCGCCTGCGC 
AGGCAGCAGG AGGAGAAGGA CAGCCAGATG AAAAGCATCA TCAGCAGGCT GATGGCTGTG 
GAAGAGGAAC TGAAGAAGGA TCATGCTGAG ATGCAAGCAG TTATTGATGC AAAGCAAAAA 
ATAATCGATG CACAGGAAAA ACGAATCGTG TCCCTGGATT CGGCCAACAC CAGACTGATG 
AGCGCACTGA CCCAAGTGAA GGAGCGGTAC AGCATGCAGG TCCGCAATGG CATCTCCCCC 
ACCAACCCCA CCAAGCTTTC CATCACGGAG AATGGTGAAT TCAAAAACAG CAGCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005622535.1
CDS	301..4560
Translation	MESWKMSIFKRQTSPCPPSYRRSLSEPAYAKSEIGISLKSYPWRGPVKIKRKKNEHAVSSLGKPLGAYRWQTHPSCRWKGGKRERLQTNKRDLDERQQKVLPEMVGSNASSSAIEVLGAPTEKLNLVNTRSLRVPFTRSISEPEPRCSIRFVRSWPTTGRSEESEQQGYFIRGIFSTLSNGFSRMLSLKGESTNEPVREDVKGPPTHRLSCGQSPYTETTTWERKYCILTDSQLVLLNKEKEIPVEGGQEQQTDSTKGRCLRRTVSVPSEGQFPEYPPEGATKLEVPAERSPRRRSISGTSTSEKPNSMDTVNTSPFKVPGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNADDRTRGLPKLKESRSHESLLSPCSAVECLDLGRGEPVSVKPLHSSILGQDFCFEVTYLSGSKCFSCNSASERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYSLPPLHSITVHIYKDVEKKKKKDKNNYVGLVNIPTASVTGRQFVEKWYPVSTPTPNKGKTGGPSIRIKSRFQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEELACALVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTIATKSIEEYLKLVGQQYLHDALGEFIKALYESDENCEVDPSKCSSSELIDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCLNRGKQDISERLISASLFLRFLCPAIMSPSLFNLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNTPGFDGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVAKLGPLPRVLADITKSLTNPTPIQQQLRRFTEHNSSPNVSGSLSSGLQKIFEDPTDSDLHKLKSPSQDNTDGYFRGKTLLLVQQASSQSMTYSEKDEKESSLPNGRSISLMDLQDTHAAQVEHASIMLDVPMRLTGSQLSITQVASIKQLRETQSTPQSAPQVRRPLHPALNQPGSLQPLSFQNPVYHLNNPIPAMPKASVDSSLENLSTASSRSQSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTVPDRHIPLALPRQNSTGQAQIRKMDQAGLGARAKAPPSLPHSASLRSTGSMSVASAALVAEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEDVEETEQNPDESKHAEKYEQEITKLKERLRVSSRRLEEYERRLLVQEQQMQKLLLEYKARLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQEKRIVSLDSANTRLMSALTQVKERYSMQVRNGISPTNPTKLSITENGEFKNSSC

Target ORF information:

RefSeq Version	XM_005622478.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005622478.2

ORF Insert Sequence:

ATGGAGTCGT GGAAGATGAG CATCTTTAAG AGACAGACAT CACCTTGTCC TCCAAGCTAT 
CGAAGATCTT TGTCAGAACC TGCTTATGCA AAATCAGAGA TAGGAATAAG CTTAAAGAGT 
TATCCATGGC GTGGTCCAGT GAAAATCAAG AGGAAGAAAA ATGAACATGC TGTTTCCAGT 
CTAGGAAAGC CACTGGGTGC TTATCGGTGG CAAACACATC CCAGCTGTAG GTGGAAAGGG 
GGCAAAAGGG AGAGGTTACA GACTAATAAA AGAGACTTGG ATGAACGGCA ACAAAAGGTC 
CTGCCAGAGA TGGTTGGTAG TAATGCTTCT TCCTCTGCTA TTGAGGTCCT TGGAGCACCA 
ACAGAGAAGC TGAACCTGGT CAACACCCGG TCACTTCGGG TTCCATTCAC CAGATCTATT 
TCAGAGCCAG AACCTCGCTG CAGTATCAGA TTTGTTAGAT CATGGCCGAC TACAGGAAGG 
TCAGAGGAGT CTGAACAGCA GGGATATTTC ATCCGTGGCA TTTTCTCTAC TCTTTCCAAT 
GGCTTCTCCA GGATGCTGAG TCTGAAAGGA GAATCAACCA ATGAGCCAGT AAGAGAAGAT 
GTGAAGGGGC CACCTACCCA TCGTCTGTCT TGTGGACAGT CGCCCTACAC AGAGACGACA 
ACATGGGAGC GGAAGTATTG CATCCTCACC GACAGCCAGT TGGTGTTGCT CAACAAGGAG 
AAGGAGATAC CTGTGGAGGG AGGACAGGAG CAGCAGACAG ATTCCACCAA AGGGCGATGC 
CTGAGGAGAA CCGTCAGTGT CCCTTCCGAG GGCCAGTTTC CTGAGTACCC ACCAGAGGGC 
GCCACCAAAC TGGAGGTGCC GGCAGAAAGG TCCCCACGCA GACGGAGTAT CTCAGGGACC 
AGTACATCAG AGAAACCCAA CTCCATGGAC ACTGTAAATA CCTCACCCTT CAAAGTACCA 
GGGTTCTTCA GCAAGCGCCT GAAAGGCTCC ATCAAGAGGA CCAAAAGCCA ATCAAAGCTT 
GACAGAAACA CGAGCTTTCG GCTACCATCC CTTCGTAATG CAGATGACAG GACTCGCGGT 
CTGCCTAAAC TAAAAGAGTC ACGTTCCCAT GAGTCATTGC TGAGCCCTTG TAGTGCAGTG 
GAATGTCTGG ATCTTGGCAG AGGAGAGCCT GTGTCAGTGA AGCCACTCCA TAGCAGCATC 
CTTGGACAAG ACTTCTGCTT TGAGGTTACG TACTTAAGTG GAAGTAAATG CTTCAGCTGT 
AACTCTGCTT CAGAGAGAGA TAAGTGGATG GAGAACCTTC GTAGGACAGT TCAACCAAAT 
AAGGACAATT GCAGACGAGC TGAAAACGTT CTTCGTTTAT GGATCATTGA AGCCAAGGAC 
CTTGCCCCCA AAAAGAAATA TTTCTGTGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT 
ACAACCAGCA AGACCAAAGC AGATAATATT TTCTGGGGTG AACATTTTGA ATTTTACAGC 
CTTCCACCTC TTCATAGCAT CACAGTTCAC ATTTATAAAG ATGTGGAAAA AAAGAAAAAA 
AAAGACAAGA ATAATTATGT AGGGCTAGTC AACATTCCCA CTGCCAGTGT GACTGGGCGC 
CAATTTGTAG AAAAGTGGTA TCCAGTGAGT ACCCCTACAC CCAACAAAGG AAAGACCGGA 
GGACCTTCTA TTCGGATTAA ATCACGTTTC CAAACTATCA CCATTCTGCC TATGGAGCAA 
TACAAAGAAT TTGCAGAATT TGTCACCAGC AACTATACCA TGCTGTGTTC TGTCCTTGAA 
CCAGTAATTA GTGTGAGAAA TAAAGAGGAG TTGGCCTGTG CCCTAGTGCA CATTCTTCAA 
AGTACTGGCA GAGCCAAGGA TTTTCTGACC GACTTGGTGA TGTCTGAGGT GGATCGCTGT 
GGAGAGCATG ACGTCTTGAT CTTCAGAGAG AACACCATTG CTACCAAATC CATTGAGGAA 
TACCTCAAGT TGGTGGGACA ACAGTATCTT CATGACGCAT TGGGGGAGTT CATCAAAGCT 
TTGTACGAGT CAGATGAGAA CTGTGAAGTG GATCCCAGCA AGTGTTCCTC TAGTGAACTG 
ATAGACCATC AGAGCAACCT GAAGATGTGC TGTGAGCTGG CTTTCTGCAA GATCATCAAC 
TCTTACTGTG TTTTCCCTCG TGAGTTGAAA GAAGTGTTTG CATCGTGGAA GCAGCAGTGC 
CTGAACCGTG GCAAACAGGA CATCAGCGAG CGGCTCATCA GTGCCTCATT GTTTCTCCGT 
TTCCTGTGCC CTGCCATCAT GTCTCCCAGT CTCTTCAACC TTATGCAAGA GTACCCTGAT 
GACCGTACAT CTCGCACCCT GACTCTGATT GCCAAGGTCA TTCAGAACCT GGCTAACTTT 
GCCAAGTTTG GTAACAAAGA AGAATATATG GCATTCATGA ATGATTTTTT AGAACATGAA 
TGGGGTGGAA TGAAGCGCTT TCTTTTGGAA ATCTCTAATC CGGACACCAT CTCAAACACC 
CCAGGCTTTG ATGGTTACAT TGATCTGGGC CGAGAGCTTT CAGTCTTGCA TTCCTTACTA 
TGGGAAGTAG TTTCCCAGCT TGATAAGGGT GAAAATTCCT TCCTACAGGC GACCGTGGCA 
AAATTGGGAC CGCTCCCTCG AGTTCTTGCT GATATTACCA AGTCTCTAAC TAATCCTACA 
CCAATACAAC AGCAGCTGAG ACGCTTCACT GAGCATAACT CCAGTCCAAA TGTCAGTGGA 
AGCCTCTCCT CTGGGCTGCA GAAAATATTT GAAGACCCCA CTGACAGTGA TTTGCATAAA 
CTAAAATCTC CAAGCCAGGA CAACACAGAT GGCTATTTCA GAGGGAAAAC GTTATTGCTG 
GTTCAGCAGG CCTCCTCCCA GAGCATGACC TATTCTGAAA AGGATGAAAA GGAAAGTAGC 
CTTCCTAACG GTCGGAGCAT CTCCCTCATG GACCTTCAAG ACACTCACGC TGCTCAAGTA 
GAGCATGCAT CTATCATGCT CGATGTGCCT ATGCGCTTAA CTGGAAGCCA GCTTTCCATA 
ACCCAGGTGG CCAGTATCAA ACAACTGCGG GAAACCCAGA GCACTCCCCA GAGTGCACCC 
CAAGTGCGAA GGCCCTTGCA CCCAGCCTTG AACCAGCCAG GCAGCCTCCA GCCCCTGTCA 
TTCCAGAACC CTGTCTATCA CCTCAATAAC CCAATCCCAG CAATGCCAAA GGCCTCTGTG 
GATTCTAGTT TGGAGAATCT AAGCACTGCC AGTTCCAGAA GCCAAAGTAA CAGTGAAGAT 
TTCAAACTCA GTGGGCCCAG CAATAGCAGC ATGGAAGATT TCACTAAACG TAGCACGCAG 
AGCGAGGATT TCTCCAGGCG GCACACTGTA CCAGACAGAC ACATACCTCT TGCTCTGCCG 
CGGCAGAATA GTACCGGGCA GGCCCAGATC CGGAAAATGG ACCAGGCTGG GTTAGGTGCC 
CGAGCCAAAG CCCCGCCGTC CTTGCCACAC AGTGCTTCCT TACGTAGCAC GGGGAGCATG 
TCGGTGGCCT CTGCGGCCCT GGTGGCTGAA CCTGTGCAGA ATGGGAGCCG GTCCCGGCAG 
CAGTCCTCTT CCTCCAGAGA GAGCCCCGTT CCCAAAGTGA GAGCGATCCA GAGACAACAG 
ACACAGCAGG TGCAGTCACC TGTGGACTCT GCCACCATGT CCCCAGTAGA GAGGACAGCA 
GCCTGGGTGT TGAACAATGG ACAGTATGAA GAGGATGTGG AAGAGACTGA GCAAAATCCA 
GATGAATCCA AGCATGCTGA GAAGTATGAG CAGGAAATTA CTAAACTGAA GGAGCGCCTG 
AGAGTGTCCA GCCGGCGCCT TGAGGAATAT GAGCGCCGCT TGCTGGTGCA GGAGCAGCAG 
ATGCAGAAGC TGCTGCTGGA ATACAAGGCC AGGCTGGAGG ACAGCGAGGA GCGCCTGCGC 
AGGCAGCAGG AGGAGAAGGA CAGCCAGATG AAAAGCATCA TCAGCAGGCT GATGGCTGTG 
GAAGAGGAAC TGAAGAAGGA TCATGCTGAG ATGCAAGCAG TTATTGATGC AAAGCAAAAA 
ATAATCGATG CACAGGAAAA ACGAATCGTG TCCCTGGATT CGGCCAACAC CAGACTGATG 
AGCGCACTGA CCCAAGTGAA GGAGCGGTAC AGCATGCAGG TCCGCAATGG CATCTCCCCC 
ACCAACCCCA CCAAGCTTTC CATCACGGAG AATGGTGAAT TCAAAAACAG CAGCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_005622478.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005622535.1 Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe09199
Clone ID Related Accession (Same CDS sequence)	XM_005622478.2 , XM_005622478.3
Accession Version	XM_005622478.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	ras GTPase-activating protein nGAP isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005622478.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTCGT GGAAGATGAG CATCTTTAAG AGACAGACAT CACCTTGTCC TCCAAGCTAT 
CGAAGATCTT TGTCAGAACC TGCTTATGCA AAATCAGAGA TAGGAATAAG CTTAAAGAGT 
TATCCATGGC GTGGTCCAGT GAAAATCAAG AGGAAGAAAA ATGAACATGC TGTTTCCAGT 
CTAGGAAAGC CACTGGGTGC TTATCGGTGG CAAACACATC CCAGCTGTAG GTGGAAAGGG 
GGCAAAAGGG AGAGGTTACA GACTAATAAA AGAGACTTGG ATGAACGGCA ACAAAAGGTC 
CTGCCAGAGA TGGTTGGTAG TAATGCTTCT TCCTCTGCTA TTGAGGTCCT TGGAGCACCA 
ACAGAGAAGC TGAACCTGGT CAACACCCGG TCACTTCGGG TTCCATTCAC CAGATCTATT 
TCAGAGCCAG AACCTCGCTG CAGTATCAGA TTTGTTAGAT CATGGCCGAC TACAGGAAGG 
TCAGAGGAGT CTGAACAGCA GGGATATTTC ATCCGTGGCA TTTTCTCTAC TCTTTCCAAT 
GGCTTCTCCA GGATGCTGAG TCTGAAAGGA GAATCAACCA ATGAGCCAGT AAGAGAAGAT 
GTGAAGGGGC CACCTACCCA TCGTCTGTCT TGTGGACAGT CGCCCTACAC AGAGACGACA 
ACATGGGAGC GGAAGTATTG CATCCTCACC GACAGCCAGT TGGTGTTGCT CAACAAGGAG 
AAGGAGATAC CTGTGGAGGG AGGACAGGAG CAGCAGACAG ATTCCACCAA AGGGCGATGC 
CTGAGGAGAA CCGTCAGTGT CCCTTCCGAG GGCCAGTTTC CTGAGTACCC ACCAGAGGGC 
GCCACCAAAC TGGAGGTGCC GGCAGAAAGG TCCCCACGCA GACGGAGTAT CTCAGGGACC 
AGTACATCAG AGAAACCCAA CTCCATGGAC ACTGTAAATA CCTCACCCTT CAAAGTACCA 
GGGTTCTTCA GCAAGCGCCT GAAAGGCTCC ATCAAGAGGA CCAAAAGCCA ATCAAAGCTT 
GACAGAAACA CGAGCTTTCG GCTACCATCC CTTCGTAATG CAGATGACAG GACTCGCGGT 
CTGCCTAAAC TAAAAGAGTC ACGTTCCCAT GAGTCATTGC TGAGCCCTTG TAGTGCAGTG 
GAATGTCTGG ATCTTGGCAG AGGAGAGCCT GTGTCAGTGA AGCCACTCCA TAGCAGCATC 
CTTGGACAAG ACTTCTGCTT TGAGGTTACG TACTTAAGTG GAAGTAAATG CTTCAGCTGT 
AACTCTGCTT CAGAGAGAGA TAAGTGGATG GAGAACCTTC GTAGGACAGT TCAACCAAAT 
AAGGACAATT GCAGACGAGC TGAAAACGTT CTTCGTTTAT GGATCATTGA AGCCAAGGAC 
CTTGCCCCCA AAAAGAAATA TTTCTGTGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT 
ACAACCAGCA AGACCAAAGC AGATAATATT TTCTGGGGTG AACATTTTGA ATTTTACAGC 
CTTCCACCTC TTCATAGCAT CACAGTTCAC ATTTATAAAG ATGTGGAAAA AAAGAAAAAA 
AAAGACAAGA ATAATTATGT AGGGCTAGTC AACATTCCCA CTGCCAGTGT GACTGGGCGC 
CAATTTGTAG AAAAGTGGTA TCCAGTGAGT ACCCCTACAC CCAACAAAGG AAAGACCGGA 
GGACCTTCTA TTCGGATTAA ATCACGTTTC CAAACTATCA CCATTCTGCC TATGGAGCAA 
TACAAAGAAT TTGCAGAATT TGTCACCAGC AACTATACCA TGCTGTGTTC TGTCCTTGAA 
CCAGTAATTA GTGTGAGAAA TAAAGAGGAG TTGGCCTGTG CCCTAGTGCA CATTCTTCAA 
AGTACTGGCA GAGCCAAGGA TTTTCTGACC GACTTGGTGA TGTCTGAGGT GGATCGCTGT 
GGAGAGCATG ACGTCTTGAT CTTCAGAGAG AACACCATTG CTACCAAATC CATTGAGGAA 
TACCTCAAGT TGGTGGGACA ACAGTATCTT CATGACGCAT TGGGGGAGTT CATCAAAGCT 
TTGTACGAGT CAGATGAGAA CTGTGAAGTG GATCCCAGCA AGTGTTCCTC TAGTGAACTG 
ATAGACCATC AGAGCAACCT GAAGATGTGC TGTGAGCTGG CTTTCTGCAA GATCATCAAC 
TCTTACTGTG TTTTCCCTCG TGAGTTGAAA GAAGTGTTTG CATCGTGGAA GCAGCAGTGC 
CTGAACCGTG GCAAACAGGA CATCAGCGAG CGGCTCATCA GTGCCTCATT GTTTCTCCGT 
TTCCTGTGCC CTGCCATCAT GTCTCCCAGT CTCTTCAACC TTATGCAAGA GTACCCTGAT 
GACCGTACAT CTCGCACCCT GACTCTGATT GCCAAGGTCA TTCAGAACCT GGCTAACTTT 
GCCAAGTTTG GTAACAAAGA AGAATATATG GCATTCATGA ATGATTTTTT AGAACATGAA 
TGGGGTGGAA TGAAGCGCTT TCTTTTGGAA ATCTCTAATC CGGACACCAT CTCAAACACC 
CCAGGCTTTG ATGGTTACAT TGATCTGGGC CGAGAGCTTT CAGTCTTGCA TTCCTTACTA 
TGGGAAGTAG TTTCCCAGCT TGATAAGGGT GAAAATTCCT TCCTACAGGC GACCGTGGCA 
AAATTGGGAC CGCTCCCTCG AGTTCTTGCT GATATTACCA AGTCTCTAAC TAATCCTACA 
CCAATACAAC AGCAGCTGAG ACGCTTCACT GAGCATAACT CCAGTCCAAA TGTCAGTGGA 
AGCCTCTCCT CTGGGCTGCA GAAAATATTT GAAGACCCCA CTGACAGTGA TTTGCATAAA 
CTAAAATCTC CAAGCCAGGA CAACACAGAT GGCTATTTCA GAGGGAAAAC GTTATTGCTG 
GTTCAGCAGG CCTCCTCCCA GAGCATGACC TATTCTGAAA AGGATGAAAA GGAAAGTAGC 
CTTCCTAACG GTCGGAGCAT CTCCCTCATG GACCTTCAAG ACACTCACGC TGCTCAAGTA 
GAGCATGCAT CTATCATGCT CGATGTGCCT ATGCGCTTAA CTGGAAGCCA GCTTTCCATA 
ACCCAGGTGG CCAGTATCAA ACAACTGCGG GAAACCCAGA GCACTCCCCA GAGTGCACCC 
CAAGTGCGAA GGCCCTTGCA CCCAGCCTTG AACCAGCCAG GCAGCCTCCA GCCCCTGTCA 
TTCCAGAACC CTGTCTATCA CCTCAATAAC CCAATCCCAG CAATGCCAAA GGCCTCTGTG 
GATTCTAGTT TGGAGAATCT AAGCACTGCC AGTTCCAGAA GCCAAAGTAA CAGTGAAGAT 
TTCAAACTCA GTGGGCCCAG CAATAGCAGC ATGGAAGATT TCACTAAACG TAGCACGCAG 
AGCGAGGATT TCTCCAGGCG GCACACTGTA CCAGACAGAC ACATACCTCT TGCTCTGCCG 
CGGCAGAATA GTACCGGGCA GGCCCAGATC CGGAAAATGG ACCAGGCTGG GTTAGGTGCC 
CGAGCCAAAG CCCCGCCGTC CTTGCCACAC AGTGCTTCCT TACGTAGCAC GGGGAGCATG 
TCGGTGGCCT CTGCGGCCCT GGTGGCTGAA CCTGTGCAGA ATGGGAGCCG GTCCCGGCAG 
CAGTCCTCTT CCTCCAGAGA GAGCCCCGTT CCCAAAGTGA GAGCGATCCA GAGACAACAG 
ACACAGCAGG TGCAGTCACC TGTGGACTCT GCCACCATGT CCCCAGTAGA GAGGACAGCA 
GCCTGGGTGT TGAACAATGG ACAGTATGAA GAGGATGTGG AAGAGACTGA GCAAAATCCA 
GATGAATCCA AGCATGCTGA GAAGTATGAG CAGGAAATTA CTAAACTGAA GGAGCGCCTG 
AGAGTGTCCA GCCGGCGCCT TGAGGAATAT GAGCGCCGCT TGCTGGTGCA GGAGCAGCAG 
ATGCAGAAGC TGCTGCTGGA ATACAAGGCC AGGCTGGAGG ACAGCGAGGA GCGCCTGCGC 
AGGCAGCAGG AGGAGAAGGA CAGCCAGATG AAAAGCATCA TCAGCAGGCT GATGGCTGTG 
GAAGAGGAAC TGAAGAAGGA TCATGCTGAG ATGCAAGCAG TTATTGATGC AAAGCAAAAA 
ATAATCGATG CACAGGAAAA ACGAATCGTG TCCCTGGATT CGGCCAACAC CAGACTGATG 
AGCGCACTGA CCCAAGTGAA GGAGCGGTAC AGCATGCAGG TCCGCAATGG CATCTCCCCC 
ACCAACCCCA CCAAGCTTTC CATCACGGAG AATGGTGAAT TCAAAAACAG CAGCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005622535.1
CDS	309..4568
Translation	MESWKMSIFKRQTSPCPPSYRRSLSEPAYAKSEIGISLKSYPWRGPVKIKRKKNEHAVSSLGKPLGAYRWQTHPSCRWKGGKRERLQTNKRDLDERQQKVLPEMVGSNASSSAIEVLGAPTEKLNLVNTRSLRVPFTRSISEPEPRCSIRFVRSWPTTGRSEESEQQGYFIRGIFSTLSNGFSRMLSLKGESTNEPVREDVKGPPTHRLSCGQSPYTETTTWERKYCILTDSQLVLLNKEKEIPVEGGQEQQTDSTKGRCLRRTVSVPSEGQFPEYPPEGATKLEVPAERSPRRRSISGTSTSEKPNSMDTVNTSPFKVPGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNADDRTRGLPKLKESRSHESLLSPCSAVECLDLGRGEPVSVKPLHSSILGQDFCFEVTYLSGSKCFSCNSASERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYSLPPLHSITVHIYKDVEKKKKKDKNNYVGLVNIPTASVTGRQFVEKWYPVSTPTPNKGKTGGPSIRIKSRFQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEELACALVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTIATKSIEEYLKLVGQQYLHDALGEFIKALYESDENCEVDPSKCSSSELIDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCLNRGKQDISERLISASLFLRFLCPAIMSPSLFNLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNTPGFDGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVAKLGPLPRVLADITKSLTNPTPIQQQLRRFTEHNSSPNVSGSLSSGLQKIFEDPTDSDLHKLKSPSQDNTDGYFRGKTLLLVQQASSQSMTYSEKDEKESSLPNGRSISLMDLQDTHAAQVEHASIMLDVPMRLTGSQLSITQVASIKQLRETQSTPQSAPQVRRPLHPALNQPGSLQPLSFQNPVYHLNNPIPAMPKASVDSSLENLSTASSRSQSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTVPDRHIPLALPRQNSTGQAQIRKMDQAGLGARAKAPPSLPHSASLRSTGSMSVASAALVAEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEDVEETEQNPDESKHAEKYEQEITKLKERLRVSSRRLEEYERRLLVQEQQMQKLLLEYKARLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQEKRIVSLDSANTRLMSALTQVKERYSMQVRNGISPTNPTKLSITENGEFKNSSC

Target ORF information:

RefSeq Version	XM_005622478.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005622478.3

ORF Insert Sequence:

ATGGAGTCGT GGAAGATGAG CATCTTTAAG AGACAGACAT CACCTTGTCC TCCAAGCTAT 
CGAAGATCTT TGTCAGAACC TGCTTATGCA AAATCAGAGA TAGGAATAAG CTTAAAGAGT 
TATCCATGGC GTGGTCCAGT GAAAATCAAG AGGAAGAAAA ATGAACATGC TGTTTCCAGT 
CTAGGAAAGC CACTGGGTGC TTATCGGTGG CAAACACATC CCAGCTGTAG GTGGAAAGGG 
GGCAAAAGGG AGAGGTTACA GACTAATAAA AGAGACTTGG ATGAACGGCA ACAAAAGGTC 
CTGCCAGAGA TGGTTGGTAG TAATGCTTCT TCCTCTGCTA TTGAGGTCCT TGGAGCACCA 
ACAGAGAAGC TGAACCTGGT CAACACCCGG TCACTTCGGG TTCCATTCAC CAGATCTATT 
TCAGAGCCAG AACCTCGCTG CAGTATCAGA TTTGTTAGAT CATGGCCGAC TACAGGAAGG 
TCAGAGGAGT CTGAACAGCA GGGATATTTC ATCCGTGGCA TTTTCTCTAC TCTTTCCAAT 
GGCTTCTCCA GGATGCTGAG TCTGAAAGGA GAATCAACCA ATGAGCCAGT AAGAGAAGAT 
GTGAAGGGGC CACCTACCCA TCGTCTGTCT TGTGGACAGT CGCCCTACAC AGAGACGACA 
ACATGGGAGC GGAAGTATTG CATCCTCACC GACAGCCAGT TGGTGTTGCT CAACAAGGAG 
AAGGAGATAC CTGTGGAGGG AGGACAGGAG CAGCAGACAG ATTCCACCAA AGGGCGATGC 
CTGAGGAGAA CCGTCAGTGT CCCTTCCGAG GGCCAGTTTC CTGAGTACCC ACCAGAGGGC 
GCCACCAAAC TGGAGGTGCC GGCAGAAAGG TCCCCACGCA GACGGAGTAT CTCAGGGACC 
AGTACATCAG AGAAACCCAA CTCCATGGAC ACTGTAAATA CCTCACCCTT CAAAGTACCA 
GGGTTCTTCA GCAAGCGCCT GAAAGGCTCC ATCAAGAGGA CCAAAAGCCA ATCAAAGCTT 
GACAGAAACA CGAGCTTTCG GCTACCATCC CTTCGTAATG CAGATGACAG GACTCGCGGT 
CTGCCTAAAC TAAAAGAGTC ACGTTCCCAT GAGTCATTGC TGAGCCCTTG TAGTGCAGTG 
GAATGTCTGG ATCTTGGCAG AGGAGAGCCT GTGTCAGTGA AGCCACTCCA TAGCAGCATC 
CTTGGACAAG ACTTCTGCTT TGAGGTTACG TACTTAAGTG GAAGTAAATG CTTCAGCTGT 
AACTCTGCTT CAGAGAGAGA TAAGTGGATG GAGAACCTTC GTAGGACAGT TCAACCAAAT 
AAGGACAATT GCAGACGAGC TGAAAACGTT CTTCGTTTAT GGATCATTGA AGCCAAGGAC 
CTTGCCCCCA AAAAGAAATA TTTCTGTGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT 
ACAACCAGCA AGACCAAAGC AGATAATATT TTCTGGGGTG AACATTTTGA ATTTTACAGC 
CTTCCACCTC TTCATAGCAT CACAGTTCAC ATTTATAAAG ATGTGGAAAA AAAGAAAAAA 
AAAGACAAGA ATAATTATGT AGGGCTAGTC AACATTCCCA CTGCCAGTGT GACTGGGCGC 
CAATTTGTAG AAAAGTGGTA TCCAGTGAGT ACCCCTACAC CCAACAAAGG AAAGACCGGA 
GGACCTTCTA TTCGGATTAA ATCACGTTTC CAAACTATCA CCATTCTGCC TATGGAGCAA 
TACAAAGAAT TTGCAGAATT TGTCACCAGC AACTATACCA TGCTGTGTTC TGTCCTTGAA 
CCAGTAATTA GTGTGAGAAA TAAAGAGGAG TTGGCCTGTG CCCTAGTGCA CATTCTTCAA 
AGTACTGGCA GAGCCAAGGA TTTTCTGACC GACTTGGTGA TGTCTGAGGT GGATCGCTGT 
GGAGAGCATG ACGTCTTGAT CTTCAGAGAG AACACCATTG CTACCAAATC CATTGAGGAA 
TACCTCAAGT TGGTGGGACA ACAGTATCTT CATGACGCAT TGGGGGAGTT CATCAAAGCT 
TTGTACGAGT CAGATGAGAA CTGTGAAGTG GATCCCAGCA AGTGTTCCTC TAGTGAACTG 
ATAGACCATC AGAGCAACCT GAAGATGTGC TGTGAGCTGG CTTTCTGCAA GATCATCAAC 
TCTTACTGTG TTTTCCCTCG TGAGTTGAAA GAAGTGTTTG CATCGTGGAA GCAGCAGTGC 
CTGAACCGTG GCAAACAGGA CATCAGCGAG CGGCTCATCA GTGCCTCATT GTTTCTCCGT 
TTCCTGTGCC CTGCCATCAT GTCTCCCAGT CTCTTCAACC TTATGCAAGA GTACCCTGAT 
GACCGTACAT CTCGCACCCT GACTCTGATT GCCAAGGTCA TTCAGAACCT GGCTAACTTT 
GCCAAGTTTG GTAACAAAGA AGAATATATG GCATTCATGA ATGATTTTTT AGAACATGAA 
TGGGGTGGAA TGAAGCGCTT TCTTTTGGAA ATCTCTAATC CGGACACCAT CTCAAACACC 
CCAGGCTTTG ATGGTTACAT TGATCTGGGC CGAGAGCTTT CAGTCTTGCA TTCCTTACTA 
TGGGAAGTAG TTTCCCAGCT TGATAAGGGT GAAAATTCCT TCCTACAGGC GACCGTGGCA 
AAATTGGGAC CGCTCCCTCG AGTTCTTGCT GATATTACCA AGTCTCTAAC TAATCCTACA 
CCAATACAAC AGCAGCTGAG ACGCTTCACT GAGCATAACT CCAGTCCAAA TGTCAGTGGA 
AGCCTCTCCT CTGGGCTGCA GAAAATATTT GAAGACCCCA CTGACAGTGA TTTGCATAAA 
CTAAAATCTC CAAGCCAGGA CAACACAGAT GGCTATTTCA GAGGGAAAAC GTTATTGCTG 
GTTCAGCAGG CCTCCTCCCA GAGCATGACC TATTCTGAAA AGGATGAAAA GGAAAGTAGC 
CTTCCTAACG GTCGGAGCAT CTCCCTCATG GACCTTCAAG ACACTCACGC TGCTCAAGTA 
GAGCATGCAT CTATCATGCT CGATGTGCCT ATGCGCTTAA CTGGAAGCCA GCTTTCCATA 
ACCCAGGTGG CCAGTATCAA ACAACTGCGG GAAACCCAGA GCACTCCCCA GAGTGCACCC 
CAAGTGCGAA GGCCCTTGCA CCCAGCCTTG AACCAGCCAG GCAGCCTCCA GCCCCTGTCA 
TTCCAGAACC CTGTCTATCA CCTCAATAAC CCAATCCCAG CAATGCCAAA GGCCTCTGTG 
GATTCTAGTT TGGAGAATCT AAGCACTGCC AGTTCCAGAA GCCAAAGTAA CAGTGAAGAT 
TTCAAACTCA GTGGGCCCAG CAATAGCAGC ATGGAAGATT TCACTAAACG TAGCACGCAG 
AGCGAGGATT TCTCCAGGCG GCACACTGTA CCAGACAGAC ACATACCTCT TGCTCTGCCG 
CGGCAGAATA GTACCGGGCA GGCCCAGATC CGGAAAATGG ACCAGGCTGG GTTAGGTGCC 
CGAGCCAAAG CCCCGCCGTC CTTGCCACAC AGTGCTTCCT TACGTAGCAC GGGGAGCATG 
TCGGTGGCCT CTGCGGCCCT GGTGGCTGAA CCTGTGCAGA ATGGGAGCCG GTCCCGGCAG 
CAGTCCTCTT CCTCCAGAGA GAGCCCCGTT CCCAAAGTGA GAGCGATCCA GAGACAACAG 
ACACAGCAGG TGCAGTCACC TGTGGACTCT GCCACCATGT CCCCAGTAGA GAGGACAGCA 
GCCTGGGTGT TGAACAATGG ACAGTATGAA GAGGATGTGG AAGAGACTGA GCAAAATCCA 
GATGAATCCA AGCATGCTGA GAAGTATGAG CAGGAAATTA CTAAACTGAA GGAGCGCCTG 
AGAGTGTCCA GCCGGCGCCT TGAGGAATAT GAGCGCCGCT TGCTGGTGCA GGAGCAGCAG 
ATGCAGAAGC TGCTGCTGGA ATACAAGGCC AGGCTGGAGG ACAGCGAGGA GCGCCTGCGC 
AGGCAGCAGG AGGAGAAGGA CAGCCAGATG AAAAGCATCA TCAGCAGGCT GATGGCTGTG 
GAAGAGGAAC TGAAGAAGGA TCATGCTGAG ATGCAAGCAG TTATTGATGC AAAGCAAAAA 
ATAATCGATG CACAGGAAAA ACGAATCGTG TCCCTGGATT CGGCCAACAC CAGACTGATG 
AGCGCACTGA CCCAAGTGAA GGAGCGGTAC AGCATGCAGG TCCGCAATGG CATCTCCCCC 
ACCAACCCCA CCAAGCTTTC CATCACGGAG AATGGTGAAT TCAAAAACAG CAGCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_005622479.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005622536.1 Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X3, mRNA.

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CloneID	OCe09200
Clone ID Related Accession (Same CDS sequence)	XM_005622479.2 , XM_005622479.3
Accession Version	XM_005622479.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4248bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	ras GTPase-activating protein nGAP isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005622479.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTCGT GGAAGATGAG CATCTTTAAG AGACAGACAT CACCTTGTCC TCCAAGCTAT 
CGAAGATCTT TGTCAGAACC TGCTTATGCA AAATCAGAGA TAGGAATAAG CTTAAAGAGT 
TATCCATGGC GTGGTCCAGT GAAAATCAAG AGGAAGAAAA ATGAACATGC TGTTTCCAGT 
CTAGGAAAGC CACTGGGTGC TTATCGGTGG CAAACACATC CCAGCTGTAG GTGGAAAGGG 
GGCAAAAGGG AGAGGTTACA GACTAATAAA AGAGACTTGG ATGAACGGCA ACAAAAGGTC 
CTGCCAGAGA TGGTTGGTAG TAATGCTTCT TCCTCTGCTA TTGAGGTCCT TGGAGCACCA 
ACAGAGAAGC TGAACCTGGT CAACACCCGG TCACTTCGGG TTCCATTCAC CAGATCTATT 
TCAGAGCCAG AACCTCGCTG CAGTATCAGA TTTGTTAGAT CATGGCCGAC TACAGGAAGG 
TCAGAGGAGT CTGAACAGCA GGGATATTTC ATCCGTGGCA TTTTCTCTAC TCTTTCCAAT 
GGCTTCTCCA GGATGCTGAG TCTGAAAGGA GAATCAACCA ATGAGCCAGT AAGAGAAGAT 
GTGAAGGGGC CACCTACCCA TCGTCTGTCT TGTGGACAGT CGCCCTACAC AGAGACGACA 
ACATGGGAGC GGAAGTATTG CATCCTCACC GACAGCCAGT TGGTGTTGCT CAACAAGGAG 
AAGGAGATAC CTGTGGAGGG AGGACAGGAG CAGCAGACAG ATTCCACCAA AGGGCGATGC 
CTGAGGAGAA CCGTCAGTGT CCCTTCCGAG GGCCAGTTTC CTGAGTACCC ACCAGAGGGC 
GCCACCAAAC TGGAGGTGCC GGCAGAAAGG TCCCCACGCA GACGGAGTAT CTCAGGGACC 
AGTACATCAG AGAAACCCAA CTCCATGGAC ACTGTAAATA CCTCACCCTT CAAAGTACCA 
GGGTTCTTCA GCAAGCGCCT GAAAGGCTCC ATCAAGAGGA CCAAAAGCCA ATCAAAGCTT 
GACAGAAACA CGAGCTTTCG GCTACCATCC CTTCGTAATG CAGATGACAG GACTCGCGGT 
CTGCCTAAAC TAAAAGAGTC ACGTTCCCAT GAGTCATTGC TGAGCCCTTG TAGTGCAGTG 
GAATGTCTGG ATCTTGGCAG AGGAGAGCCT GTGTCAGTGA AGCCACTCCA TAGCAGCATC 
CTTGGACAAG ACTTCTGCTT TGAGGTTACG TACTTAAGTG GAAGTAAATG CTTCAGCTGT 
AACTCTGCTT CAGAGAGAGA TAAGTGGATG GAGAACCTTC GTAGGACAGT TCAACCAAAT 
AAGGACAATT GCAGACGAGC TGAAAACGTT CTTCGTTTAT GGATCATTGA AGCCAAGGAC 
CTTGCCCCCA AAAAGAAATA TTTCTGTGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT 
ACAACCAGCA AGACCAAAGC AGATAATATT TTCTGGGGTG AACATTTTGA ATTTTACAGC 
CTTCCACCTC TTCATAGCAT CACAGTTCAC ATTTATAAAG ATGTGGAAAA AAAGAAAAAA 
AAAGACAAGA ATAATTATGT AGGGCTAGTC AACATTCCCA CTGCCAGTGT GACTGGGCGC 
CAATTTGTAG AAAAGTGGTA TCCAGTGAGT ACCCCTACAC CCAACAAAGG AAAGACCGGA 
GGACCTTCTA TTCGGATTAA ATCACGTTTC CAAACTATCA CCATTCTGCC TATGGAGCAA 
TACAAAGAAT TTGCAGAATT TGTCACCAGC AACTATACCA TGCTGTGTTC TGTCCTTGAA 
CCAGTAATTA GTGTGAGAAA TAAAGAGGAG TTGGCCTGTG CCCTAGTGCA CATTCTTCAA 
AGTACTGGCA GAGCCAAGGA TTTTCTGACC GACTTGGTGA TGTCTGAGGT GGATCGCTGT 
GGAGAGCATG ACGTCTTGAT CTTCAGAGAG AACACCATTG CTACCAAATC CATTGAGGAA 
TACCTCAAGT TGGTGGGACA ACAGTATCTT CATGACGCAT TGGGGGAGTT CATCAAAGCT 
TTGTACGAGT CAGATGAGAA CTGTGAAGTG GATCCCAGCA AGTGTTCCTC TAGTGAACTG 
ATAGACCATC AGAGCAACCT GAAGATGTGC TGTGAGCTGG CTTTCTGCAA GATCATCAAC 
TCTTACTGTG TTTTCCCTCG TGAGTTGAAA GAAGTGTTTG CATCGTGGAA GCAGCAGTGC 
CTGAACCGTG GCAAACAGGA CATCAGCGAG CGGCTCATCA GTGCCTCATT GTTTCTCCGT 
TTCCTGTGCC CTGCCATCAT GTCTCCCAGT CTCTTCAACC TTATGCAAGA GTACCCTGAT 
GACCGTACAT CTCGCACCCT GACTCTGATT GCCAAGGTCA TTCAGAACCT GGCTAACTTT 
GCCAAGTTTG GTAACAAAGA AGAATATATG GCATTCATGA ATGATTTTTT AGAACATGAA 
TGGGGTGGAA TGAAGCGCTT TCTTTTGGAA ATCTCTAATC CGGACACCAT CTCAAACACC 
CCAGGCTTTG ATGGTTACAT TGATCTGGGC CGAGAGCTTT CAGTCTTGCA TTCCTTACTA 
TGGGAAGTAG TTTCCCAGCT TGATAAGGCG ACCGTGGCAA AATTGGGACC GCTCCCTCGA 
GTTCTTGCTG ATATTACCAA GTCTCTAACT AATCCTACAC CAATACAACA GCAGCTGAGA 
CGCTTCACTG AGCATAACTC CAGTCCAAAT GTCAGTGGAA GCCTCTCCTC TGGGCTGCAG 
AAAATATTTG AAGACCCCAC TGACAGTGAT TTGCATAAAC TAAAATCTCC AAGCCAGGAC 
AACACAGATG GCTATTTCAG AGGGAAAACG TTATTGCTGG TTCAGCAGGC CTCCTCCCAG 
AGCATGACCT ATTCTGAAAA GGATGAAAAG GAAAGTAGCC TTCCTAACGG TCGGAGCATC 
TCCCTCATGG ACCTTCAAGA CACTCACGCT GCTCAAGTAG AGCATGCATC TATCATGCTC 
GATGTGCCTA TGCGCTTAAC TGGAAGCCAG CTTTCCATAA CCCAGGTGGC CAGTATCAAA 
CAACTGCGGG AAACCCAGAG CACTCCCCAG AGTGCACCCC AAGTGCGAAG GCCCTTGCAC 
CCAGCCTTGA ACCAGCCAGG CAGCCTCCAG CCCCTGTCAT TCCAGAACCC TGTCTATCAC 
CTCAATAACC CAATCCCAGC AATGCCAAAG GCCTCTGTGG ATTCTAGTTT GGAGAATCTA 
AGCACTGCCA GTTCCAGAAG CCAAAGTAAC AGTGAAGATT TCAAACTCAG TGGGCCCAGC 
AATAGCAGCA TGGAAGATTT CACTAAACGT AGCACGCAGA GCGAGGATTT CTCCAGGCGG 
CACACTGTAC CAGACAGACA CATACCTCTT GCTCTGCCGC GGCAGAATAG TACCGGGCAG 
GCCCAGATCC GGAAAATGGA CCAGGCTGGG TTAGGTGCCC GAGCCAAAGC CCCGCCGTCC 
TTGCCACACA GTGCTTCCTT ACGTAGCACG GGGAGCATGT CGGTGGCCTC TGCGGCCCTG 
GTGGCTGAAC CTGTGCAGAA TGGGAGCCGG TCCCGGCAGC AGTCCTCTTC CTCCAGAGAG 
AGCCCCGTTC CCAAAGTGAG AGCGATCCAG AGACAACAGA CACAGCAGGT GCAGTCACCT 
GTGGACTCTG CCACCATGTC CCCAGTAGAG AGGACAGCAG CCTGGGTGTT GAACAATGGA 
CAGTATGAAG AGGATGTGGA AGAGACTGAG CAAAATCCAG ATGAATCCAA GCATGCTGAG 
AAGTATGAGC AGGAAATTAC TAAACTGAAG GAGCGCCTGA GAGTGTCCAG CCGGCGCCTT 
GAGGAATATG AGCGCCGCTT GCTGGTGCAG GAGCAGCAGA TGCAGAAGCT GCTGCTGGAA 
TACAAGGCCA GGCTGGAGGA CAGCGAGGAG CGCCTGCGCA GGCAGCAGGA GGAGAAGGAC 
AGCCAGATGA AAAGCATCAT CAGCAGGCTG ATGGCTGTGG AAGAGGAACT GAAGAAGGAT 
CATGCTGAGA TGCAAGCAGT TATTGATGCA AAGCAAAAAA TAATCGATGC ACAGGTCATA 
AATGGAGATG AGATTATTCA AACAGGAAAA ACGAATCGTG TCCCTGGATT CGGCCAACAC 
CAGACTGATG AGCGCACTGA CCCAAGTGAA GGAGCGGTAC AGCATGCAGG TCCGCAATGG 
CATCTCCCCC ACCAACCCCA CCAAGCTTTC CATCACGGAG AATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005622536.1
CDS	303..4550
Translation	MESWKMSIFKRQTSPCPPSYRRSLSEPAYAKSEIGISLKSYPWRGPVKIKRKKNEHAVSSLGKPLGAYRWQTHPSCRWKGGKRERLQTNKRDLDERQQKVLPEMVGSNASSSAIEVLGAPTEKLNLVNTRSLRVPFTRSISEPEPRCSIRFVRSWPTTGRSEESEQQGYFIRGIFSTLSNGFSRMLSLKGESTNEPVREDVKGPPTHRLSCGQSPYTETTTWERKYCILTDSQLVLLNKEKEIPVEGGQEQQTDSTKGRCLRRTVSVPSEGQFPEYPPEGATKLEVPAERSPRRRSISGTSTSEKPNSMDTVNTSPFKVPGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNADDRTRGLPKLKESRSHESLLSPCSAVECLDLGRGEPVSVKPLHSSILGQDFCFEVTYLSGSKCFSCNSASERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYSLPPLHSITVHIYKDVEKKKKKDKNNYVGLVNIPTASVTGRQFVEKWYPVSTPTPNKGKTGGPSIRIKSRFQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEELACALVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTIATKSIEEYLKLVGQQYLHDALGEFIKALYESDENCEVDPSKCSSSELIDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCLNRGKQDISERLISASLFLRFLCPAIMSPSLFNLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNTPGFDGYIDLGRELSVLHSLLWEVVSQLDKATVAKLGPLPRVLADITKSLTNPTPIQQQLRRFTEHNSSPNVSGSLSSGLQKIFEDPTDSDLHKLKSPSQDNTDGYFRGKTLLLVQQASSQSMTYSEKDEKESSLPNGRSISLMDLQDTHAAQVEHASIMLDVPMRLTGSQLSITQVASIKQLRETQSTPQSAPQVRRPLHPALNQPGSLQPLSFQNPVYHLNNPIPAMPKASVDSSLENLSTASSRSQSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTVPDRHIPLALPRQNSTGQAQIRKMDQAGLGARAKAPPSLPHSASLRSTGSMSVASAALVAEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEDVEETEQNPDESKHAEKYEQEITKLKERLRVSSRRLEEYERRLLVQEQQMQKLLLEYKARLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQVINGDEIIQTGKTNRVPGFGQHQTDERTDPSEGAVQHAGPQWHLPHQPHQAFHHGEW

Target ORF information:

RefSeq Version	XM_005622479.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005622479.2

ORF Insert Sequence:

ATGGAGTCGT GGAAGATGAG CATCTTTAAG AGACAGACAT CACCTTGTCC TCCAAGCTAT 
CGAAGATCTT TGTCAGAACC TGCTTATGCA AAATCAGAGA TAGGAATAAG CTTAAAGAGT 
TATCCATGGC GTGGTCCAGT GAAAATCAAG AGGAAGAAAA ATGAACATGC TGTTTCCAGT 
CTAGGAAAGC CACTGGGTGC TTATCGGTGG CAAACACATC CCAGCTGTAG GTGGAAAGGG 
GGCAAAAGGG AGAGGTTACA GACTAATAAA AGAGACTTGG ATGAACGGCA ACAAAAGGTC 
CTGCCAGAGA TGGTTGGTAG TAATGCTTCT TCCTCTGCTA TTGAGGTCCT TGGAGCACCA 
ACAGAGAAGC TGAACCTGGT CAACACCCGG TCACTTCGGG TTCCATTCAC CAGATCTATT 
TCAGAGCCAG AACCTCGCTG CAGTATCAGA TTTGTTAGAT CATGGCCGAC TACAGGAAGG 
TCAGAGGAGT CTGAACAGCA GGGATATTTC ATCCGTGGCA TTTTCTCTAC TCTTTCCAAT 
GGCTTCTCCA GGATGCTGAG TCTGAAAGGA GAATCAACCA ATGAGCCAGT AAGAGAAGAT 
GTGAAGGGGC CACCTACCCA TCGTCTGTCT TGTGGACAGT CGCCCTACAC AGAGACGACA 
ACATGGGAGC GGAAGTATTG CATCCTCACC GACAGCCAGT TGGTGTTGCT CAACAAGGAG 
AAGGAGATAC CTGTGGAGGG AGGACAGGAG CAGCAGACAG ATTCCACCAA AGGGCGATGC 
CTGAGGAGAA CCGTCAGTGT CCCTTCCGAG GGCCAGTTTC CTGAGTACCC ACCAGAGGGC 
GCCACCAAAC TGGAGGTGCC GGCAGAAAGG TCCCCACGCA GACGGAGTAT CTCAGGGACC 
AGTACATCAG AGAAACCCAA CTCCATGGAC ACTGTAAATA CCTCACCCTT CAAAGTACCA 
GGGTTCTTCA GCAAGCGCCT GAAAGGCTCC ATCAAGAGGA CCAAAAGCCA ATCAAAGCTT 
GACAGAAACA CGAGCTTTCG GCTACCATCC CTTCGTAATG CAGATGACAG GACTCGCGGT 
CTGCCTAAAC TAAAAGAGTC ACGTTCCCAT GAGTCATTGC TGAGCCCTTG TAGTGCAGTG 
GAATGTCTGG ATCTTGGCAG AGGAGAGCCT GTGTCAGTGA AGCCACTCCA TAGCAGCATC 
CTTGGACAAG ACTTCTGCTT TGAGGTTACG TACTTAAGTG GAAGTAAATG CTTCAGCTGT 
AACTCTGCTT CAGAGAGAGA TAAGTGGATG GAGAACCTTC GTAGGACAGT TCAACCAAAT 
AAGGACAATT GCAGACGAGC TGAAAACGTT CTTCGTTTAT GGATCATTGA AGCCAAGGAC 
CTTGCCCCCA AAAAGAAATA TTTCTGTGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT 
ACAACCAGCA AGACCAAAGC AGATAATATT TTCTGGGGTG AACATTTTGA ATTTTACAGC 
CTTCCACCTC TTCATAGCAT CACAGTTCAC ATTTATAAAG ATGTGGAAAA AAAGAAAAAA 
AAAGACAAGA ATAATTATGT AGGGCTAGTC AACATTCCCA CTGCCAGTGT GACTGGGCGC 
CAATTTGTAG AAAAGTGGTA TCCAGTGAGT ACCCCTACAC CCAACAAAGG AAAGACCGGA 
GGACCTTCTA TTCGGATTAA ATCACGTTTC CAAACTATCA CCATTCTGCC TATGGAGCAA 
TACAAAGAAT TTGCAGAATT TGTCACCAGC AACTATACCA TGCTGTGTTC TGTCCTTGAA 
CCAGTAATTA GTGTGAGAAA TAAAGAGGAG TTGGCCTGTG CCCTAGTGCA CATTCTTCAA 
AGTACTGGCA GAGCCAAGGA TTTTCTGACC GACTTGGTGA TGTCTGAGGT GGATCGCTGT 
GGAGAGCATG ACGTCTTGAT CTTCAGAGAG AACACCATTG CTACCAAATC CATTGAGGAA 
TACCTCAAGT TGGTGGGACA ACAGTATCTT CATGACGCAT TGGGGGAGTT CATCAAAGCT 
TTGTACGAGT CAGATGAGAA CTGTGAAGTG GATCCCAGCA AGTGTTCCTC TAGTGAACTG 
ATAGACCATC AGAGCAACCT GAAGATGTGC TGTGAGCTGG CTTTCTGCAA GATCATCAAC 
TCTTACTGTG TTTTCCCTCG TGAGTTGAAA GAAGTGTTTG CATCGTGGAA GCAGCAGTGC 
CTGAACCGTG GCAAACAGGA CATCAGCGAG CGGCTCATCA GTGCCTCATT GTTTCTCCGT 
TTCCTGTGCC CTGCCATCAT GTCTCCCAGT CTCTTCAACC TTATGCAAGA GTACCCTGAT 
GACCGTACAT CTCGCACCCT GACTCTGATT GCCAAGGTCA TTCAGAACCT GGCTAACTTT 
GCCAAGTTTG GTAACAAAGA AGAATATATG GCATTCATGA ATGATTTTTT AGAACATGAA 
TGGGGTGGAA TGAAGCGCTT TCTTTTGGAA ATCTCTAATC CGGACACCAT CTCAAACACC 
CCAGGCTTTG ATGGTTACAT TGATCTGGGC CGAGAGCTTT CAGTCTTGCA TTCCTTACTA 
TGGGAAGTAG TTTCCCAGCT TGATAAGGCG ACCGTGGCAA AATTGGGACC GCTCCCTCGA 
GTTCTTGCTG ATATTACCAA GTCTCTAACT AATCCTACAC CAATACAACA GCAGCTGAGA 
CGCTTCACTG AGCATAACTC CAGTCCAAAT GTCAGTGGAA GCCTCTCCTC TGGGCTGCAG 
AAAATATTTG AAGACCCCAC TGACAGTGAT TTGCATAAAC TAAAATCTCC AAGCCAGGAC 
AACACAGATG GCTATTTCAG AGGGAAAACG TTATTGCTGG TTCAGCAGGC CTCCTCCCAG 
AGCATGACCT ATTCTGAAAA GGATGAAAAG GAAAGTAGCC TTCCTAACGG TCGGAGCATC 
TCCCTCATGG ACCTTCAAGA CACTCACGCT GCTCAAGTAG AGCATGCATC TATCATGCTC 
GATGTGCCTA TGCGCTTAAC TGGAAGCCAG CTTTCCATAA CCCAGGTGGC CAGTATCAAA 
CAACTGCGGG AAACCCAGAG CACTCCCCAG AGTGCACCCC AAGTGCGAAG GCCCTTGCAC 
CCAGCCTTGA ACCAGCCAGG CAGCCTCCAG CCCCTGTCAT TCCAGAACCC TGTCTATCAC 
CTCAATAACC CAATCCCAGC AATGCCAAAG GCCTCTGTGG ATTCTAGTTT GGAGAATCTA 
AGCACTGCCA GTTCCAGAAG CCAAAGTAAC AGTGAAGATT TCAAACTCAG TGGGCCCAGC 
AATAGCAGCA TGGAAGATTT CACTAAACGT AGCACGCAGA GCGAGGATTT CTCCAGGCGG 
CACACTGTAC CAGACAGACA CATACCTCTT GCTCTGCCGC GGCAGAATAG TACCGGGCAG 
GCCCAGATCC GGAAAATGGA CCAGGCTGGG TTAGGTGCCC GAGCCAAAGC CCCGCCGTCC 
TTGCCACACA GTGCTTCCTT ACGTAGCACG GGGAGCATGT CGGTGGCCTC TGCGGCCCTG 
GTGGCTGAAC CTGTGCAGAA TGGGAGCCGG TCCCGGCAGC AGTCCTCTTC CTCCAGAGAG 
AGCCCCGTTC CCAAAGTGAG AGCGATCCAG AGACAACAGA CACAGCAGGT GCAGTCACCT 
GTGGACTCTG CCACCATGTC CCCAGTAGAG AGGACAGCAG CCTGGGTGTT GAACAATGGA 
CAGTATGAAG AGGATGTGGA AGAGACTGAG CAAAATCCAG ATGAATCCAA GCATGCTGAG 
AAGTATGAGC AGGAAATTAC TAAACTGAAG GAGCGCCTGA GAGTGTCCAG CCGGCGCCTT 
GAGGAATATG AGCGCCGCTT GCTGGTGCAG GAGCAGCAGA TGCAGAAGCT GCTGCTGGAA 
TACAAGGCCA GGCTGGAGGA CAGCGAGGAG CGCCTGCGCA GGCAGCAGGA GGAGAAGGAC 
AGCCAGATGA AAAGCATCAT CAGCAGGCTG ATGGCTGTGG AAGAGGAACT GAAGAAGGAT 
CATGCTGAGA TGCAAGCAGT TATTGATGCA AAGCAAAAAA TAATCGATGC ACAGGTCATA 
AATGGAGATG AGATTATTCA AACAGGAAAA ACGAATCGTG TCCCTGGATT CGGCCAACAC 
CAGACTGATG AGCGCACTGA CCCAAGTGAA GGAGCGGTAC AGCATGCAGG TCCGCAATGG 
CATCTCCCCC ACCAACCCCA CCAAGCTTTC CATCACGGAG AATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_005622479.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005622536.1 Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X3, mRNA.

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CloneID	OCe09200
Clone ID Related Accession (Same CDS sequence)	XM_005622479.2 , XM_005622479.3
Accession Version	XM_005622479.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4248bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	ras GTPase-activating protein nGAP isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005622479.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTCGT GGAAGATGAG CATCTTTAAG AGACAGACAT CACCTTGTCC TCCAAGCTAT 
CGAAGATCTT TGTCAGAACC TGCTTATGCA AAATCAGAGA TAGGAATAAG CTTAAAGAGT 
TATCCATGGC GTGGTCCAGT GAAAATCAAG AGGAAGAAAA ATGAACATGC TGTTTCCAGT 
CTAGGAAAGC CACTGGGTGC TTATCGGTGG CAAACACATC CCAGCTGTAG GTGGAAAGGG 
GGCAAAAGGG AGAGGTTACA GACTAATAAA AGAGACTTGG ATGAACGGCA ACAAAAGGTC 
CTGCCAGAGA TGGTTGGTAG TAATGCTTCT TCCTCTGCTA TTGAGGTCCT TGGAGCACCA 
ACAGAGAAGC TGAACCTGGT CAACACCCGG TCACTTCGGG TTCCATTCAC CAGATCTATT 
TCAGAGCCAG AACCTCGCTG CAGTATCAGA TTTGTTAGAT CATGGCCGAC TACAGGAAGG 
TCAGAGGAGT CTGAACAGCA GGGATATTTC ATCCGTGGCA TTTTCTCTAC TCTTTCCAAT 
GGCTTCTCCA GGATGCTGAG TCTGAAAGGA GAATCAACCA ATGAGCCAGT AAGAGAAGAT 
GTGAAGGGGC CACCTACCCA TCGTCTGTCT TGTGGACAGT CGCCCTACAC AGAGACGACA 
ACATGGGAGC GGAAGTATTG CATCCTCACC GACAGCCAGT TGGTGTTGCT CAACAAGGAG 
AAGGAGATAC CTGTGGAGGG AGGACAGGAG CAGCAGACAG ATTCCACCAA AGGGCGATGC 
CTGAGGAGAA CCGTCAGTGT CCCTTCCGAG GGCCAGTTTC CTGAGTACCC ACCAGAGGGC 
GCCACCAAAC TGGAGGTGCC GGCAGAAAGG TCCCCACGCA GACGGAGTAT CTCAGGGACC 
AGTACATCAG AGAAACCCAA CTCCATGGAC ACTGTAAATA CCTCACCCTT CAAAGTACCA 
GGGTTCTTCA GCAAGCGCCT GAAAGGCTCC ATCAAGAGGA CCAAAAGCCA ATCAAAGCTT 
GACAGAAACA CGAGCTTTCG GCTACCATCC CTTCGTAATG CAGATGACAG GACTCGCGGT 
CTGCCTAAAC TAAAAGAGTC ACGTTCCCAT GAGTCATTGC TGAGCCCTTG TAGTGCAGTG 
GAATGTCTGG ATCTTGGCAG AGGAGAGCCT GTGTCAGTGA AGCCACTCCA TAGCAGCATC 
CTTGGACAAG ACTTCTGCTT TGAGGTTACG TACTTAAGTG GAAGTAAATG CTTCAGCTGT 
AACTCTGCTT CAGAGAGAGA TAAGTGGATG GAGAACCTTC GTAGGACAGT TCAACCAAAT 
AAGGACAATT GCAGACGAGC TGAAAACGTT CTTCGTTTAT GGATCATTGA AGCCAAGGAC 
CTTGCCCCCA AAAAGAAATA TTTCTGTGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT 
ACAACCAGCA AGACCAAAGC AGATAATATT TTCTGGGGTG AACATTTTGA ATTTTACAGC 
CTTCCACCTC TTCATAGCAT CACAGTTCAC ATTTATAAAG ATGTGGAAAA AAAGAAAAAA 
AAAGACAAGA ATAATTATGT AGGGCTAGTC AACATTCCCA CTGCCAGTGT GACTGGGCGC 
CAATTTGTAG AAAAGTGGTA TCCAGTGAGT ACCCCTACAC CCAACAAAGG AAAGACCGGA 
GGACCTTCTA TTCGGATTAA ATCACGTTTC CAAACTATCA CCATTCTGCC TATGGAGCAA 
TACAAAGAAT TTGCAGAATT TGTCACCAGC AACTATACCA TGCTGTGTTC TGTCCTTGAA 
CCAGTAATTA GTGTGAGAAA TAAAGAGGAG TTGGCCTGTG CCCTAGTGCA CATTCTTCAA 
AGTACTGGCA GAGCCAAGGA TTTTCTGACC GACTTGGTGA TGTCTGAGGT GGATCGCTGT 
GGAGAGCATG ACGTCTTGAT CTTCAGAGAG AACACCATTG CTACCAAATC CATTGAGGAA 
TACCTCAAGT TGGTGGGACA ACAGTATCTT CATGACGCAT TGGGGGAGTT CATCAAAGCT 
TTGTACGAGT CAGATGAGAA CTGTGAAGTG GATCCCAGCA AGTGTTCCTC TAGTGAACTG 
ATAGACCATC AGAGCAACCT GAAGATGTGC TGTGAGCTGG CTTTCTGCAA GATCATCAAC 
TCTTACTGTG TTTTCCCTCG TGAGTTGAAA GAAGTGTTTG CATCGTGGAA GCAGCAGTGC 
CTGAACCGTG GCAAACAGGA CATCAGCGAG CGGCTCATCA GTGCCTCATT GTTTCTCCGT 
TTCCTGTGCC CTGCCATCAT GTCTCCCAGT CTCTTCAACC TTATGCAAGA GTACCCTGAT 
GACCGTACAT CTCGCACCCT GACTCTGATT GCCAAGGTCA TTCAGAACCT GGCTAACTTT 
GCCAAGTTTG GTAACAAAGA AGAATATATG GCATTCATGA ATGATTTTTT AGAACATGAA 
TGGGGTGGAA TGAAGCGCTT TCTTTTGGAA ATCTCTAATC CGGACACCAT CTCAAACACC 
CCAGGCTTTG ATGGTTACAT TGATCTGGGC CGAGAGCTTT CAGTCTTGCA TTCCTTACTA 
TGGGAAGTAG TTTCCCAGCT TGATAAGGCG ACCGTGGCAA AATTGGGACC GCTCCCTCGA 
GTTCTTGCTG ATATTACCAA GTCTCTAACT AATCCTACAC CAATACAACA GCAGCTGAGA 
CGCTTCACTG AGCATAACTC CAGTCCAAAT GTCAGTGGAA GCCTCTCCTC TGGGCTGCAG 
AAAATATTTG AAGACCCCAC TGACAGTGAT TTGCATAAAC TAAAATCTCC AAGCCAGGAC 
AACACAGATG GCTATTTCAG AGGGAAAACG TTATTGCTGG TTCAGCAGGC CTCCTCCCAG 
AGCATGACCT ATTCTGAAAA GGATGAAAAG GAAAGTAGCC TTCCTAACGG TCGGAGCATC 
TCCCTCATGG ACCTTCAAGA CACTCACGCT GCTCAAGTAG AGCATGCATC TATCATGCTC 
GATGTGCCTA TGCGCTTAAC TGGAAGCCAG CTTTCCATAA CCCAGGTGGC CAGTATCAAA 
CAACTGCGGG AAACCCAGAG CACTCCCCAG AGTGCACCCC AAGTGCGAAG GCCCTTGCAC 
CCAGCCTTGA ACCAGCCAGG CAGCCTCCAG CCCCTGTCAT TCCAGAACCC TGTCTATCAC 
CTCAATAACC CAATCCCAGC AATGCCAAAG GCCTCTGTGG ATTCTAGTTT GGAGAATCTA 
AGCACTGCCA GTTCCAGAAG CCAAAGTAAC AGTGAAGATT TCAAACTCAG TGGGCCCAGC 
AATAGCAGCA TGGAAGATTT CACTAAACGT AGCACGCAGA GCGAGGATTT CTCCAGGCGG 
CACACTGTAC CAGACAGACA CATACCTCTT GCTCTGCCGC GGCAGAATAG TACCGGGCAG 
GCCCAGATCC GGAAAATGGA CCAGGCTGGG TTAGGTGCCC GAGCCAAAGC CCCGCCGTCC 
TTGCCACACA GTGCTTCCTT ACGTAGCACG GGGAGCATGT CGGTGGCCTC TGCGGCCCTG 
GTGGCTGAAC CTGTGCAGAA TGGGAGCCGG TCCCGGCAGC AGTCCTCTTC CTCCAGAGAG 
AGCCCCGTTC CCAAAGTGAG AGCGATCCAG AGACAACAGA CACAGCAGGT GCAGTCACCT 
GTGGACTCTG CCACCATGTC CCCAGTAGAG AGGACAGCAG CCTGGGTGTT GAACAATGGA 
CAGTATGAAG AGGATGTGGA AGAGACTGAG CAAAATCCAG ATGAATCCAA GCATGCTGAG 
AAGTATGAGC AGGAAATTAC TAAACTGAAG GAGCGCCTGA GAGTGTCCAG CCGGCGCCTT 
GAGGAATATG AGCGCCGCTT GCTGGTGCAG GAGCAGCAGA TGCAGAAGCT GCTGCTGGAA 
TACAAGGCCA GGCTGGAGGA CAGCGAGGAG CGCCTGCGCA GGCAGCAGGA GGAGAAGGAC 
AGCCAGATGA AAAGCATCAT CAGCAGGCTG ATGGCTGTGG AAGAGGAACT GAAGAAGGAT 
CATGCTGAGA TGCAAGCAGT TATTGATGCA AAGCAAAAAA TAATCGATGC ACAGGTCATA 
AATGGAGATG AGATTATTCA AACAGGAAAA ACGAATCGTG TCCCTGGATT CGGCCAACAC 
CAGACTGATG AGCGCACTGA CCCAAGTGAA GGAGCGGTAC AGCATGCAGG TCCGCAATGG 
CATCTCCCCC ACCAACCCCA CCAAGCTTTC CATCACGGAG AATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005622536.1
CDS	311..4558
Translation	MESWKMSIFKRQTSPCPPSYRRSLSEPAYAKSEIGISLKSYPWRGPVKIKRKKNEHAVSSLGKPLGAYRWQTHPSCRWKGGKRERLQTNKRDLDERQQKVLPEMVGSNASSSAIEVLGAPTEKLNLVNTRSLRVPFTRSISEPEPRCSIRFVRSWPTTGRSEESEQQGYFIRGIFSTLSNGFSRMLSLKGESTNEPVREDVKGPPTHRLSCGQSPYTETTTWERKYCILTDSQLVLLNKEKEIPVEGGQEQQTDSTKGRCLRRTVSVPSEGQFPEYPPEGATKLEVPAERSPRRRSISGTSTSEKPNSMDTVNTSPFKVPGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNADDRTRGLPKLKESRSHESLLSPCSAVECLDLGRGEPVSVKPLHSSILGQDFCFEVTYLSGSKCFSCNSASERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYSLPPLHSITVHIYKDVEKKKKKDKNNYVGLVNIPTASVTGRQFVEKWYPVSTPTPNKGKTGGPSIRIKSRFQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEELACALVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTIATKSIEEYLKLVGQQYLHDALGEFIKALYESDENCEVDPSKCSSSELIDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCLNRGKQDISERLISASLFLRFLCPAIMSPSLFNLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNTPGFDGYIDLGRELSVLHSLLWEVVSQLDKATVAKLGPLPRVLADITKSLTNPTPIQQQLRRFTEHNSSPNVSGSLSSGLQKIFEDPTDSDLHKLKSPSQDNTDGYFRGKTLLLVQQASSQSMTYSEKDEKESSLPNGRSISLMDLQDTHAAQVEHASIMLDVPMRLTGSQLSITQVASIKQLRETQSTPQSAPQVRRPLHPALNQPGSLQPLSFQNPVYHLNNPIPAMPKASVDSSLENLSTASSRSQSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTVPDRHIPLALPRQNSTGQAQIRKMDQAGLGARAKAPPSLPHSASLRSTGSMSVASAALVAEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEDVEETEQNPDESKHAEKYEQEITKLKERLRVSSRRLEEYERRLLVQEQQMQKLLLEYKARLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQVINGDEIIQTGKTNRVPGFGQHQTDERTDPSEGAVQHAGPQWHLPHQPHQAFHHGEW

Target ORF information:

RefSeq Version	XM_005622479.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris RAS protein activator like 2 (RASAL2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005622479.3

ORF Insert Sequence:

ATGGAGTCGT GGAAGATGAG CATCTTTAAG AGACAGACAT CACCTTGTCC TCCAAGCTAT 
CGAAGATCTT TGTCAGAACC TGCTTATGCA AAATCAGAGA TAGGAATAAG CTTAAAGAGT 
TATCCATGGC GTGGTCCAGT GAAAATCAAG AGGAAGAAAA ATGAACATGC TGTTTCCAGT 
CTAGGAAAGC CACTGGGTGC TTATCGGTGG CAAACACATC CCAGCTGTAG GTGGAAAGGG 
GGCAAAAGGG AGAGGTTACA GACTAATAAA AGAGACTTGG ATGAACGGCA ACAAAAGGTC 
CTGCCAGAGA TGGTTGGTAG TAATGCTTCT TCCTCTGCTA TTGAGGTCCT TGGAGCACCA 
ACAGAGAAGC TGAACCTGGT CAACACCCGG TCACTTCGGG TTCCATTCAC CAGATCTATT 
TCAGAGCCAG AACCTCGCTG CAGTATCAGA TTTGTTAGAT CATGGCCGAC TACAGGAAGG 
TCAGAGGAGT CTGAACAGCA GGGATATTTC ATCCGTGGCA TTTTCTCTAC TCTTTCCAAT 
GGCTTCTCCA GGATGCTGAG TCTGAAAGGA GAATCAACCA ATGAGCCAGT AAGAGAAGAT 
GTGAAGGGGC CACCTACCCA TCGTCTGTCT TGTGGACAGT CGCCCTACAC AGAGACGACA 
ACATGGGAGC GGAAGTATTG CATCCTCACC GACAGCCAGT TGGTGTTGCT CAACAAGGAG 
AAGGAGATAC CTGTGGAGGG AGGACAGGAG CAGCAGACAG ATTCCACCAA AGGGCGATGC 
CTGAGGAGAA CCGTCAGTGT CCCTTCCGAG GGCCAGTTTC CTGAGTACCC ACCAGAGGGC 
GCCACCAAAC TGGAGGTGCC GGCAGAAAGG TCCCCACGCA GACGGAGTAT CTCAGGGACC 
AGTACATCAG AGAAACCCAA CTCCATGGAC ACTGTAAATA CCTCACCCTT CAAAGTACCA 
GGGTTCTTCA GCAAGCGCCT GAAAGGCTCC ATCAAGAGGA CCAAAAGCCA ATCAAAGCTT 
GACAGAAACA CGAGCTTTCG GCTACCATCC CTTCGTAATG CAGATGACAG GACTCGCGGT 
CTGCCTAAAC TAAAAGAGTC ACGTTCCCAT GAGTCATTGC TGAGCCCTTG TAGTGCAGTG 
GAATGTCTGG ATCTTGGCAG AGGAGAGCCT GTGTCAGTGA AGCCACTCCA TAGCAGCATC 
CTTGGACAAG ACTTCTGCTT TGAGGTTACG TACTTAAGTG GAAGTAAATG CTTCAGCTGT 
AACTCTGCTT CAGAGAGAGA TAAGTGGATG GAGAACCTTC GTAGGACAGT TCAACCAAAT 
AAGGACAATT GCAGACGAGC TGAAAACGTT CTTCGTTTAT GGATCATTGA AGCCAAGGAC 
CTTGCCCCCA AAAAGAAATA TTTCTGTGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT 
ACAACCAGCA AGACCAAAGC AGATAATATT TTCTGGGGTG AACATTTTGA ATTTTACAGC 
CTTCCACCTC TTCATAGCAT CACAGTTCAC ATTTATAAAG ATGTGGAAAA AAAGAAAAAA 
AAAGACAAGA ATAATTATGT AGGGCTAGTC AACATTCCCA CTGCCAGTGT GACTGGGCGC 
CAATTTGTAG AAAAGTGGTA TCCAGTGAGT ACCCCTACAC CCAACAAAGG AAAGACCGGA 
GGACCTTCTA TTCGGATTAA ATCACGTTTC CAAACTATCA CCATTCTGCC TATGGAGCAA 
TACAAAGAAT TTGCAGAATT TGTCACCAGC AACTATACCA TGCTGTGTTC TGTCCTTGAA 
CCAGTAATTA GTGTGAGAAA TAAAGAGGAG TTGGCCTGTG CCCTAGTGCA CATTCTTCAA 
AGTACTGGCA GAGCCAAGGA TTTTCTGACC GACTTGGTGA TGTCTGAGGT GGATCGCTGT 
GGAGAGCATG ACGTCTTGAT CTTCAGAGAG AACACCATTG CTACCAAATC CATTGAGGAA 
TACCTCAAGT TGGTGGGACA ACAGTATCTT CATGACGCAT TGGGGGAGTT CATCAAAGCT 
TTGTACGAGT CAGATGAGAA CTGTGAAGTG GATCCCAGCA AGTGTTCCTC TAGTGAACTG 
ATAGACCATC AGAGCAACCT GAAGATGTGC TGTGAGCTGG CTTTCTGCAA GATCATCAAC 
TCTTACTGTG TTTTCCCTCG TGAGTTGAAA GAAGTGTTTG CATCGTGGAA GCAGCAGTGC 
CTGAACCGTG GCAAACAGGA CATCAGCGAG CGGCTCATCA GTGCCTCATT GTTTCTCCGT 
TTCCTGTGCC CTGCCATCAT GTCTCCCAGT CTCTTCAACC TTATGCAAGA GTACCCTGAT 
GACCGTACAT CTCGCACCCT GACTCTGATT GCCAAGGTCA TTCAGAACCT GGCTAACTTT 
GCCAAGTTTG GTAACAAAGA AGAATATATG GCATTCATGA ATGATTTTTT AGAACATGAA 
TGGGGTGGAA TGAAGCGCTT TCTTTTGGAA ATCTCTAATC CGGACACCAT CTCAAACACC 
CCAGGCTTTG ATGGTTACAT TGATCTGGGC CGAGAGCTTT CAGTCTTGCA TTCCTTACTA 
TGGGAAGTAG TTTCCCAGCT TGATAAGGCG ACCGTGGCAA AATTGGGACC GCTCCCTCGA 
GTTCTTGCTG ATATTACCAA GTCTCTAACT AATCCTACAC CAATACAACA GCAGCTGAGA 
CGCTTCACTG AGCATAACTC CAGTCCAAAT GTCAGTGGAA GCCTCTCCTC TGGGCTGCAG 
AAAATATTTG AAGACCCCAC TGACAGTGAT TTGCATAAAC TAAAATCTCC AAGCCAGGAC 
AACACAGATG GCTATTTCAG AGGGAAAACG TTATTGCTGG TTCAGCAGGC CTCCTCCCAG 
AGCATGACCT ATTCTGAAAA GGATGAAAAG GAAAGTAGCC TTCCTAACGG TCGGAGCATC 
TCCCTCATGG ACCTTCAAGA CACTCACGCT GCTCAAGTAG AGCATGCATC TATCATGCTC 
GATGTGCCTA TGCGCTTAAC TGGAAGCCAG CTTTCCATAA CCCAGGTGGC CAGTATCAAA 
CAACTGCGGG AAACCCAGAG CACTCCCCAG AGTGCACCCC AAGTGCGAAG GCCCTTGCAC 
CCAGCCTTGA ACCAGCCAGG CAGCCTCCAG CCCCTGTCAT TCCAGAACCC TGTCTATCAC 
CTCAATAACC CAATCCCAGC AATGCCAAAG GCCTCTGTGG ATTCTAGTTT GGAGAATCTA 
AGCACTGCCA GTTCCAGAAG CCAAAGTAAC AGTGAAGATT TCAAACTCAG TGGGCCCAGC 
AATAGCAGCA TGGAAGATTT CACTAAACGT AGCACGCAGA GCGAGGATTT CTCCAGGCGG 
CACACTGTAC CAGACAGACA CATACCTCTT GCTCTGCCGC GGCAGAATAG TACCGGGCAG 
GCCCAGATCC GGAAAATGGA CCAGGCTGGG TTAGGTGCCC GAGCCAAAGC CCCGCCGTCC 
TTGCCACACA GTGCTTCCTT ACGTAGCACG GGGAGCATGT CGGTGGCCTC TGCGGCCCTG 
GTGGCTGAAC CTGTGCAGAA TGGGAGCCGG TCCCGGCAGC AGTCCTCTTC CTCCAGAGAG 
AGCCCCGTTC CCAAAGTGAG AGCGATCCAG AGACAACAGA CACAGCAGGT GCAGTCACCT 
GTGGACTCTG CCACCATGTC CCCAGTAGAG AGGACAGCAG CCTGGGTGTT GAACAATGGA 
CAGTATGAAG AGGATGTGGA AGAGACTGAG CAAAATCCAG ATGAATCCAA GCATGCTGAG 
AAGTATGAGC AGGAAATTAC TAAACTGAAG GAGCGCCTGA GAGTGTCCAG CCGGCGCCTT 
GAGGAATATG AGCGCCGCTT GCTGGTGCAG GAGCAGCAGA TGCAGAAGCT GCTGCTGGAA 
TACAAGGCCA GGCTGGAGGA CAGCGAGGAG CGCCTGCGCA GGCAGCAGGA GGAGAAGGAC 
AGCCAGATGA AAAGCATCAT CAGCAGGCTG ATGGCTGTGG AAGAGGAACT GAAGAAGGAT 
CATGCTGAGA TGCAAGCAGT TATTGATGCA AAGCAAAAAA TAATCGATGC ACAGGTCATA 
AATGGAGATG AGATTATTCA AACAGGAAAA ACGAATCGTG TCCCTGGATT CGGCCAACAC 
CAGACTGATG AGCGCACTGA CCCAAGTGAA GGAGCGGTAC AGCATGCAGG TCCGCAATGG 
CATCTCCCCC ACCAACCCCA CCAAGCTTTC CATCACGGAG AATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	RASAL2
Entrez Gene ID	480056
Full Name	RAS protein activator like 2
General protein information	Preferred Names RAS protein activator like 2 Names ras GTPase-activating protein nGAP
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

Danio rerio (zebrafish)	rasal2	XP_001332688.5
Xenopus tropicalis (tropical clawed frog)	rasal2	XP_004914087.1
Homo sapiens (human)	RASAL2	NP_733793.2
Gallus gallus (chicken)	RASAL2	XP_004943302.1
Canis lupus familiaris (dog)	RASAL2	XP_005622535.1
Bos taurus (cattle)	RASAL2	XP_005217296.1
Pan troglodytes (chimpanzee)	RASAL2	XP_003949696.1
Mus musculus (house mouse)	Rasal2	NP_808312.3
	RASAL2	XP_002802086.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

RASAL2 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

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