RASAL2 cDNA ORF clone, Gallus gallus(chicken)

The following RASAL2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RASAL2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa152361 XM_015290510.2
Latest version!
Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OGa152362 XM_004943245.3
Latest version!
Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OGa152363 XM_015290511.2
Latest version!
Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OGa152364 XM_004943246.3
Latest version!
Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OGa152365 XM_015290512.2
Latest version!
Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OGa152366 XM_015290513.2
Latest version!
Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OGa152367 XM_015290514.2
Latest version!
Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OGa152368 XM_015290515.2
Latest version!
Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OGa152369 XM_015290516.2
Latest version!
Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OGa152370 XM_004943254.3
Latest version!
Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OGa152371 XM_015290519.2
Latest version!
Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OGa152372 XM_015290520.2
Latest version!
Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OGa39039 XM_015290510.1
Latest version!
Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa39040 XM_004943245.2
Latest version!
Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa39041 XM_015290511.1
Latest version!
Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa39042 XM_004943246.2
Latest version!
Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa39043 XM_015290512.1
Latest version!
Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa39047 XM_015290515.1
Latest version!
Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa39048 XM_015290516.1
Latest version!
Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa39049 XM_004943254.2
Latest version!
Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa39050 XM_015290519.1
Latest version!
Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa39051 XM_015290520.1
Latest version!
Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa11742 XM_004943250.3
Latest version!
Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OGa39044 XM_015290513.1
Latest version!
Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OGa39045 XM_004943250.2
Latest version!
Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OGa39046 XM_015290514.1
Latest version!
Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa152361
    Clone ID Related Accession (Same CDS sequence) XM_015290510.2
    Accession Version XM_015290510.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4296bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product ras GTPase-activating protein nGAP isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006095.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015290510.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAGTGCTT TTAAAAAGTA TTACAGTCTC TCTCCCAGGA CCTTTAGGAG GTCTTTGTCG 
    GAGCAAGATG CTGGCACCGC TGCCTTAAAG GACTATAATT GGTACCACCA AATGAGAAGC
    AAGAAGGGGA GCAAACAAAC AGCTTTGGAC CGAGAGAAAG CACTCGGAGC GTACAGGTGG
    CAAACACATC CCCGTCTTCC CTGGAGGACA AGCAGAAGAG CACACTCTTG CAGCCTCAGA
    GGGGAAGGTA ATGATTCGAG TCTTGTCAAA AGTTCTGCAA GCATGGATGC TACCAGCGCC
    GACGATCTCC ACCAAGACTC AACGGAGATG CTCTTCCAGG CAAGGACCAG ATCACCGCGG
    GTTCCCTTCA ACCGCTCCCT GTCTGAACCT GTGCCGCACT CTGCAAACAG GAATGCCAAG
    CCCTTTCTGC AGGCGGTGCG CTCTGTGGAT TGTGAGCAGC ACAGCTATTT TGTCCGTGGC
    ATCTTCTCAA CTCTCTCAAG TGGCTTTTCA AAGATAATAA ATCGAAGGGG TGAGCAGCCC
    CCCACTGAGC CACTGGAGGA GTGGAAACCA GCCGGTAGTC AAATTGACCC GTGCCCAGGT
    AATGGACTCT GTTCTGTTTG TGTTACAGAT GTAAAAGGAC CACCTACGCA CAGGCTGTCC
    TGCGGCCAGT CCCCCTACAC CGAGACGACA ACATGGGAGA GGAAGTACTG CATCCTGACT
    GACAGCCAGC TGGTGCTGCT CAACAGGGAG AAGGAGGTGC CCACCGAGGG GTTGCAGGAG
    CCGCAGGATG CAACCAAAGG CCGCTGTCTG CGCCGCACGG TGAGCGTGCC CTCCGAGGGC
    CAGTTCCCCG AGTACCCACC AGATGGGGCC GCCAAGCTGG AGGTCCCGGC AGAGAGATCT
    CCTCGCAGAC GGAGCATCTC TGGGACCAGC ACATCTGAGA AAACCAACTC CATGGATGCT
    GCAAATACCT CTCCTTTCAA AGTATCGGGC TTCTTCAGTA AACGTCTGAA AGGGTCCATC
    AAGAGGACAA AGAGTCAGTC AAAGCTGGAC AGGAACACAA GTTTCCGCCT CCCATCCCTC
    CGCAATGCTG ATGACAGGTC ACGTGGGCTG CCGAAGCTGA AGGAGTCCCG CTCTCATGAG
    TCCCTGCTGA GCCCCGGCAG CGCAGTGGAA GCCCTGGACC TTGGGGCAGA AGAAAAGGTC
    TTTGTAAAGC CACTGCACAG CAGCATCCTC GGACAGGACT TCTGCTTTGA GGTAACCTAC
    TCCAGCGGCA GTAAATGCTT CAGCTGTTCC TCTGCTGCAG AGAGGGATAA GTGGATGGAA
    AACCTACGCA GAACAGTGCA GCCGAATAAG GACAACTGCC GTCGAGCTGA AAATGTGCTC
    CGTCTCTGGA TCATTGAGGC AAAGGACCTG GCCCCCAAGA AGAAATACTT CTGTGAGCTG
    TGCCTTGATG ACACTCTCTT TGCCCGCACC ACCAGCAAAA CGAAAGCAGA TAACATTTTC
    TGGGGGGAGC ACTTTGAGTT CTACGGCCTC CCAGCTCTTC ACAGCATCAC GGTTCACATC
    TACAAGGATG TGGAGAAGAA AAAGAAAAAG GACAAAAACA ATTACGTCGG CCTGGTCAAC
    ATTCCCATGG CCACTGTAAC GGGGCGCCAG TTTGTGGAAA AATGGTACCC AGTCAGCACA
    CCTACACCCA ACAAAGGGAA ATCAGGAGGC CCTTCCATTC GCATCAAGTC GCGTTATCAG
    ACCATCACCA TCTTACCCAT GGAGCAATAC AAGGAGTTTG CAGAGTTTGT TACCAGCAAC
    TACACGATGC TGTGCTCTGT GCTGGAGCCA GTCATCAGCG TAAGGAATAA GGAAGAGATG
    GCTTGTGCAA TGGTGCACAT TCTTCAGAGC ACGGGGAGAG CCAAGGACTT CCTAACGGAT
    CTGGTGATGT CTGAGGTGGA TCGCTGCGGG GAACACGATG TCTTGATCTT CAGGGAGAAC
    ACACTTGCTA CCAAAGCTAT TGAGGAATAC CTGAAGTTGG TAGGGCAGAA GTATCTCCAT
    GATGCGCTAG GGGAGTTTAT CAAGGCTTTG TATGAGTCAG ATGAAAACTG TGAAGTGGAC
    CCCAGCAAAT GTTCATCCAG TGAATTAACA GACCATCAGA GCAACCTGAA AATGTGTTGT
    GAGCTGGCCT TCTGCAAGAT TATCAATTCC TACTGCGTGT TCCCTCGTGA GCTGAAGGAG
    GTGTTTGCAT CTTGGAAGCA GCAGTGCCTG AGCAGGGGCA AGCAGGACAT CAGCGAGCGG
    CTGATCAGTG CCTCCCTGTT CCTCCGCTTC CTGTGCCCCG CCATCATGTC CCCCAGCCTC
    TTCAGCCTCA TGCAGGAGTA TCCTGACGAC CGGACATCGC GCACGCTCAC ACTCATTGCT
    AAAGTCATTC AGAATCTCGC CAATTTTGCT AAGTTTGGTA ACAAGGAAGA GTACATGGCT
    TTCATGAACG ACTTTCTGGA ACATGAGTGG GGGGGAATGA AGCGATTTCT CCTGGAGATC
    TCTAATCCAG ATACCATCTC AAACATGCCT GGATTTGAGG GCTATATCGA CCTGGGCAGA
    GAGCTCTCAG TTTTGCATTC CCTCTTATGG GAGGTTGTGT CCCAACTTGA TAAGGGTGAA
    AATTCCTTCC TACAGGCGAC CGTGGCAAAA CTGGGACCTC TTCCTCGTAT TCTTGCCGAC
    ATCACCAAAT CAATGACCAA CCCTACGCCA ATACAGCAGC AGCTGAGGCG TTTCACTGAG
    CACAGCTCTA GTCCAAACGT CAGTGGCAGT CTCTCCTCAG GGCTTCAGAA GATATTCGAG
    GACCCCACTG ATGGTGACTT GCATAAACTG AAGTCTCCAA CCCAGGAAAA CATAGATGGC
    TATTTCAGGG GCAAGACCTT GCTGTTGGTT CAGCAGGCGT CCACCCAGAG CATGACTTAC
    TCAGATAAGG ACGAAAGAGA GAGCATCTTG CCCAACGGAC GTAGCATCTC CCTCATGGAC
    CTGCAGGATC CCCATACCGC TCACAGCGAC CACGCTTCCA TGATGCACGA CGTGCCTTTG
    CGCTTGGCCG GCAGCCAGCT CTCCATCACC CAGGTGGCCA ACATCAAGCA GCTGTGGGAG
    ACCCAGAGCA CGCCCCAAAG CGCTCCCCAG GTGAGGAGGC CGCTGCACCC GGCTTTGAAC
    CAGCAGGGCA GCCTCCAGCC TCTGTCATTC CAGAACCCGG TGTACCACCT CAACAACCCA
    CCTGCGCCGT TGCCCAAGGC CTCCGTGGAC TCCAGCCTGG AGAACCTGAG CACCGCCAGC
    TCCCAGAGCC GAAGCAACAG CGAAGACTTC AAGCTCAGCG GGCCCAGCAA CAGCAGCATG
    GAGGACTTCA CCAAGCGCAG CACGCAGAGC GAGGACTTCT CTAGGCGGCA CACGGTCCCG
    GACAAGCACG TCCCCATCGC GTTGCCCCGC CAGAACAGCA GCAGCCAGGC ACAGATCCGC
    AAAATGGACC AGGCCGGGTT AGGGGCCAGG GCCAAAGCGC CGCAGTCGCT GCCGCACAGC
    GCGTCCCTGC GCAGCACGGG CAGCATGTCG GCCGTGTCGG GGGCCGTGGT GACTGAGCCC
    GTCCAGAACG GCAGCCGCTC CCGGCAGCAG TCGTCCTCCT CCAGGGAGAG CCCTGTGCCG
    AAGGTGAGGG CCATTCAGAG GCAGCAAACG CAGCAGGTCC AGTCTCCTGT GGACTCAGCC
    ACAATGTCAC CAGTGGAAAG GACAGCAGCG TGGGTTCTCA ATAACGGGCA GTACGAGGAG
    GTCGAGGAGG ACACAGAGCA GAACCCAGAT GAAGCCAAGC ATGCTGAGAA GTACGAACAA
    GAGATAGCAA AGCTGAAGGA ACGCTTGAAA GTGTCGAGCC GCCGGCTGGA GGAGTACGAG
    CGGCGGCTCC TGGTGCAGGA GCAGCAAATG CAGAAGCTGC TGATGGAGTA CAAGTCCAGG
    CTGGAGGACA GCGAGGAGAG ACTGCGCAGG CAACAGGAGG AGAAGGACAG CCAGATGAAA
    AGCATTATCA GCAGGCTCAT GGCAGTTGAA GAGGAACTGA AGAAAGATCA TGCAGAGATG
    CAAGCTGTCA TCGATGCAAA GCAGAAAATT ATTGATGCAC AGGTCATTAA TGGGAATGAG
    ATAATTCAAA CAGGAGAAGC GGATCGTGTC CCTGGACTCT GCCAACACGC GGCTGATGAG
    TGCCCTGACG CAGGTGAAGG AGCGCTACAG CATGCAAGTC CGCAATGGCA TCTCCCCCAC
    CAACCCCACC AAGCTTTCCA TCACGGAGAA TGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015145996.1
    CDS637..4932
    Translation

    Target ORF information:

    RefSeq Version XM_015290510.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015290510.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAGTGCTT TTAAAAAGTA TTACAGTCTC TCTCCCAGGA CCTTTAGGAG GTCTTTGTCG 
    GAGCAAGATG CTGGCACCGC TGCCTTAAAG GACTATAATT GGTACCACCA AATGAGAAGC
    AAGAAGGGGA GCAAACAAAC AGCTTTGGAC CGAGAGAAAG CACTCGGAGC GTACAGGTGG
    CAAACACATC CCCGTCTTCC CTGGAGGACA AGCAGAAGAG CACACTCTTG CAGCCTCAGA
    GGGGAAGGTA ATGATTCGAG TCTTGTCAAA AGTTCTGCAA GCATGGATGC TACCAGCGCC
    GACGATCTCC ACCAAGACTC AACGGAGATG CTCTTCCAGG CAAGGACCAG ATCACCGCGG
    GTTCCCTTCA ACCGCTCCCT GTCTGAACCT GTGCCGCACT CTGCAAACAG GAATGCCAAG
    CCCTTTCTGC AGGCGGTGCG CTCTGTGGAT TGTGAGCAGC ACAGCTATTT TGTCCGTGGC
    ATCTTCTCAA CTCTCTCAAG TGGCTTTTCA AAGATAATAA ATCGAAGGGG TGAGCAGCCC
    CCCACTGAGC CACTGGAGGA GTGGAAACCA GCCGGTAGTC AAATTGACCC GTGCCCAGGT
    AATGGACTCT GTTCTGTTTG TGTTACAGAT GTAAAAGGAC CACCTACGCA CAGGCTGTCC
    TGCGGCCAGT CCCCCTACAC CGAGACGACA ACATGGGAGA GGAAGTACTG CATCCTGACT
    GACAGCCAGC TGGTGCTGCT CAACAGGGAG AAGGAGGTGC CCACCGAGGG GTTGCAGGAG
    CCGCAGGATG CAACCAAAGG CCGCTGTCTG CGCCGCACGG TGAGCGTGCC CTCCGAGGGC
    CAGTTCCCCG AGTACCCACC AGATGGGGCC GCCAAGCTGG AGGTCCCGGC AGAGAGATCT
    CCTCGCAGAC GGAGCATCTC TGGGACCAGC ACATCTGAGA AAACCAACTC CATGGATGCT
    GCAAATACCT CTCCTTTCAA AGTATCGGGC TTCTTCAGTA AACGTCTGAA AGGGTCCATC
    AAGAGGACAA AGAGTCAGTC AAAGCTGGAC AGGAACACAA GTTTCCGCCT CCCATCCCTC
    CGCAATGCTG ATGACAGGTC ACGTGGGCTG CCGAAGCTGA AGGAGTCCCG CTCTCATGAG
    TCCCTGCTGA GCCCCGGCAG CGCAGTGGAA GCCCTGGACC TTGGGGCAGA AGAAAAGGTC
    TTTGTAAAGC CACTGCACAG CAGCATCCTC GGACAGGACT TCTGCTTTGA GGTAACCTAC
    TCCAGCGGCA GTAAATGCTT CAGCTGTTCC TCTGCTGCAG AGAGGGATAA GTGGATGGAA
    AACCTACGCA GAACAGTGCA GCCGAATAAG GACAACTGCC GTCGAGCTGA AAATGTGCTC
    CGTCTCTGGA TCATTGAGGC AAAGGACCTG GCCCCCAAGA AGAAATACTT CTGTGAGCTG
    TGCCTTGATG ACACTCTCTT TGCCCGCACC ACCAGCAAAA CGAAAGCAGA TAACATTTTC
    TGGGGGGAGC ACTTTGAGTT CTACGGCCTC CCAGCTCTTC ACAGCATCAC GGTTCACATC
    TACAAGGATG TGGAGAAGAA AAAGAAAAAG GACAAAAACA ATTACGTCGG CCTGGTCAAC
    ATTCCCATGG CCACTGTAAC GGGGCGCCAG TTTGTGGAAA AATGGTACCC AGTCAGCACA
    CCTACACCCA ACAAAGGGAA ATCAGGAGGC CCTTCCATTC GCATCAAGTC GCGTTATCAG
    ACCATCACCA TCTTACCCAT GGAGCAATAC AAGGAGTTTG CAGAGTTTGT TACCAGCAAC
    TACACGATGC TGTGCTCTGT GCTGGAGCCA GTCATCAGCG TAAGGAATAA GGAAGAGATG
    GCTTGTGCAA TGGTGCACAT TCTTCAGAGC ACGGGGAGAG CCAAGGACTT CCTAACGGAT
    CTGGTGATGT CTGAGGTGGA TCGCTGCGGG GAACACGATG TCTTGATCTT CAGGGAGAAC
    ACACTTGCTA CCAAAGCTAT TGAGGAATAC CTGAAGTTGG TAGGGCAGAA GTATCTCCAT
    GATGCGCTAG GGGAGTTTAT CAAGGCTTTG TATGAGTCAG ATGAAAACTG TGAAGTGGAC
    CCCAGCAAAT GTTCATCCAG TGAATTAACA GACCATCAGA GCAACCTGAA AATGTGTTGT
    GAGCTGGCCT TCTGCAAGAT TATCAATTCC TACTGCGTGT TCCCTCGTGA GCTGAAGGAG
    GTGTTTGCAT CTTGGAAGCA GCAGTGCCTG AGCAGGGGCA AGCAGGACAT CAGCGAGCGG
    CTGATCAGTG CCTCCCTGTT CCTCCGCTTC CTGTGCCCCG CCATCATGTC CCCCAGCCTC
    TTCAGCCTCA TGCAGGAGTA TCCTGACGAC CGGACATCGC GCACGCTCAC ACTCATTGCT
    AAAGTCATTC AGAATCTCGC CAATTTTGCT AAGTTTGGTA ACAAGGAAGA GTACATGGCT
    TTCATGAACG ACTTTCTGGA ACATGAGTGG GGGGGAATGA AGCGATTTCT CCTGGAGATC
    TCTAATCCAG ATACCATCTC AAACATGCCT GGATTTGAGG GCTATATCGA CCTGGGCAGA
    GAGCTCTCAG TTTTGCATTC CCTCTTATGG GAGGTTGTGT CCCAACTTGA TAAGGGTGAA
    AATTCCTTCC TACAGGCGAC CGTGGCAAAA CTGGGACCTC TTCCTCGTAT TCTTGCCGAC
    ATCACCAAAT CAATGACCAA CCCTACGCCA ATACAGCAGC AGCTGAGGCG TTTCACTGAG
    CACAGCTCTA GTCCAAACGT CAGTGGCAGT CTCTCCTCAG GGCTTCAGAA GATATTCGAG
    GACCCCACTG ATGGTGACTT GCATAAACTG AAGTCTCCAA CCCAGGAAAA CATAGATGGC
    TATTTCAGGG GCAAGACCTT GCTGTTGGTT CAGCAGGCGT CCACCCAGAG CATGACTTAC
    TCAGATAAGG ACGAAAGAGA GAGCATCTTG CCCAACGGAC GTAGCATCTC CCTCATGGAC
    CTGCAGGATC CCCATACCGC TCACAGCGAC CACGCTTCCA TGATGCACGA CGTGCCTTTG
    CGCTTGGCCG GCAGCCAGCT CTCCATCACC CAGGTGGCCA ACATCAAGCA GCTGTGGGAG
    ACCCAGAGCA CGCCCCAAAG CGCTCCCCAG GTGAGGAGGC CGCTGCACCC GGCTTTGAAC
    CAGCAGGGCA GCCTCCAGCC TCTGTCATTC CAGAACCCGG TGTACCACCT CAACAACCCA
    CCTGCGCCGT TGCCCAAGGC CTCCGTGGAC TCCAGCCTGG AGAACCTGAG CACCGCCAGC
    TCCCAGAGCC GAAGCAACAG CGAAGACTTC AAGCTCAGCG GGCCCAGCAA CAGCAGCATG
    GAGGACTTCA CCAAGCGCAG CACGCAGAGC GAGGACTTCT CTAGGCGGCA CACGGTCCCG
    GACAAGCACG TCCCCATCGC GTTGCCCCGC CAGAACAGCA GCAGCCAGGC ACAGATCCGC
    AAAATGGACC AGGCCGGGTT AGGGGCCAGG GCCAAAGCGC CGCAGTCGCT GCCGCACAGC
    GCGTCCCTGC GCAGCACGGG CAGCATGTCG GCCGTGTCGG GGGCCGTGGT GACTGAGCCC
    GTCCAGAACG GCAGCCGCTC CCGGCAGCAG TCGTCCTCCT CCAGGGAGAG CCCTGTGCCG
    AAGGTGAGGG CCATTCAGAG GCAGCAAACG CAGCAGGTCC AGTCTCCTGT GGACTCAGCC
    ACAATGTCAC CAGTGGAAAG GACAGCAGCG TGGGTTCTCA ATAACGGGCA GTACGAGGAG
    GTCGAGGAGG ACACAGAGCA GAACCCAGAT GAAGCCAAGC ATGCTGAGAA GTACGAACAA
    GAGATAGCAA AGCTGAAGGA ACGCTTGAAA GTGTCGAGCC GCCGGCTGGA GGAGTACGAG
    CGGCGGCTCC TGGTGCAGGA GCAGCAAATG CAGAAGCTGC TGATGGAGTA CAAGTCCAGG
    CTGGAGGACA GCGAGGAGAG ACTGCGCAGG CAACAGGAGG AGAAGGACAG CCAGATGAAA
    AGCATTATCA GCAGGCTCAT GGCAGTTGAA GAGGAACTGA AGAAAGATCA TGCAGAGATG
    CAAGCTGTCA TCGATGCAAA GCAGAAAATT ATTGATGCAC AGGTCATTAA TGGGAATGAG
    ATAATTCAAA CAGGAGAAGC GGATCGTGTC CCTGGACTCT GCCAACACGC GGCTGATGAG
    TGCCCTGACG CAGGTGAAGG AGCGCTACAG CATGCAAGTC CGCAATGGCA TCTCCCCCAC
    CAACCCCACC AAGCTTTCCA TCACGGAGAA TGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa152362
    Clone ID Related Accession (Same CDS sequence) XM_004943245.3
    Accession Version XM_004943245.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4287bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product ras GTPase-activating protein nGAP isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006095.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004943245.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAGTGCTT TTAAAAAGTA TTACAGTCTC TCTCCCAGGA CCTTTAGGAG GTCTTTGTCG 
    GAGCAAGATG CTGGCACCGC TGCCTTAAAG GACTATAATT GGTACCACCA AATGAGAAGC
    AAGAAGGGGA GCAAACAAAC AGCTTTGGAC CGAGAGAAAG CACTCGGAGC GTACAGGTGG
    CAAACACATC CCCGTCTTCC CTGGAGGACA AGCAGAAGAG CACACTCTTG CAGCCTCAGA
    GGGGAAGGTA ATGATTCGAG TCTTGTCAAA AGTTCTGCAA GCATGGATGC TACCAGCGCC
    GACGATCTCC ACCAAGACTC AACGGAGATG CTCTTCCAGG CAAGGACCAG ATCACCGCGG
    GTTCCCTTCA ACCGCTCCCT GTCTGAACCT GTGCCGCACT CTGCAAACAG GAATGCCAAG
    CCCTTTCTGC AGGCGGTGCG CTCTGTGGAT TGTGAGCAGC ACAGCTATTT TGTCCGTGGC
    ATCTTCTCAA CTCTCTCAAG TGGCTTTTCA AAGATAATAA ATCGAAGGGG TGAGCAGCCC
    CCCACTGAGC CACTGGAGGA GTGGAAACCA GCCGGTAGTC AAATTGACCC GTGCCCAGGT
    AATGGACTCT GTTCTGTTTG TGTTACAGAT GTAAAAGGAC CACCTACGCA CAGGCTGTCC
    TGCGGCCAGT CCCCCTACAC CGAGACGACA ACATGGGAGA GGAAGTACTG CATCCTGACT
    GACAGCCAGC TGGTGCTGCT CAACAGGGAG AAGGAGGTGC CCACCGAGGG GTTGCAGGAG
    CCGCAGGATG CAACCAAAGG CCGCTGTCTG CGCCGCACGG TGAGCGTGCC CTCCGAGGGC
    CAGTTCCCCG AGTACCCACC AGATGGGGCC GCCAAGCTGG AGGTCCCGGC AGAGAGATCT
    CCTCGCAGAC GGAGCATCTC TGGGACCAGC ACATCTGAGA AAACCAACTC CATGGATGCT
    GCAAATACCT CTCCTTTCAA AGTATCGGGC TTCTTCAGTA AACGTCTGAA AGGGTCCATC
    AAGAGGACAA AGAGTCAGTC AAAGCTGGAC AGGAACACAA GTTTCCGCCT CCCATCCCTC
    CGCAATGCTG ATGACAGGTC ACGTGGGCTG CCGAAGCTGA AGGAGTCCCG CTCTCATGAG
    TCCCTGCTGA GCCCCGGCAG CGCAGTGGAA GCCCTGGACC TTGGGGCAGA AGAAAAGGTC
    TTTGTAAAGC CACTGCACAG CAGCATCCTC GGACAGGACT TCTGCTTTGA GGTAACCTAC
    TCCAGCGGCA GTAAATGCTT CAGCTGTTCC TCTGCTGCAG AGAGGGATAA GTGGATGGAA
    AACCTACGCA GAACAGTGCA GCCGAATAAG GACAACTGCC GTCGAGCTGA AAATGTGCTC
    CGTCTCTGGA TCATTGAGGC AAAGGACCTG GCCCCCAAGA AGAAATACTT CTGTGAGCTG
    TGCCTTGATG ACACTCTCTT TGCCCGCACC ACCAGCAAAA CGAAAGCAGA TAACATTTTC
    TGGGGGGAGC ACTTTGAGTT CTACGGCCTC CCAGCTCTTC ACAGCATCAC GGTTCACATC
    TACAAGGATG TGGAGAAGAA AAAGAAAAAG GACAAAAACA ATTACGTCGG CCTGGTCAAC
    ATTCCCATGG CCACTGTAAC GGGGCGCCAG TTTGTGGAAA AATGGTACCC AGTCAGCACA
    CCTACACCCA ACAAAGGGAA ATCAGGAGGC CCTTCCATTC GCATCAAGTC GCGTTATCAG
    ACCATCACCA TCTTACCCAT GGAGCAATAC AAGGAGTTTG CAGAGTTTGT TACCAGCAAC
    TACACGATGC TGTGCTCTGT GCTGGAGCCA GTCATCAGCG TAAGGAATAA GGAAGAGATG
    GCTTGTGCAA TGGTGCACAT TCTTCAGAGC ACGGGGAGAG CCAAGGACTT CCTAACGGAT
    CTGGTGATGT CTGAGGTGGA TCGCTGCGGG GAACACGATG TCTTGATCTT CAGGGAGAAC
    ACACTTGCTA CCAAAGCTAT TGAGGAATAC CTGAAGTTGG TAGGGCAGAA GTATCTCCAT
    GATGCGCTAG GGGAGTTTAT CAAGGCTTTG TATGAGTCAG ATGAAAACTG TGAAGTGGAC
    CCCAGCAAAT GTTCATCCAG TGAATTAACA GACCATCAGA GCAACCTGAA AATGTGTTGT
    GAGCTGGCCT TCTGCAAGAT TATCAATTCC TACTGCGTGT TCCCTCGTGA GCTGAAGGAG
    GTGTTTGCAT CTTGGAAGCA GCAGTGCCTG AGCAGGGGCA AGCAGGACAT CAGCGAGCGG
    CTGATCAGTG CCTCCCTGTT CCTCCGCTTC CTGTGCCCCG CCATCATGTC CCCCAGCCTC
    TTCAGCCTCA TGCAGGAGTA TCCTGACGAC CGGACATCGC GCACGCTCAC ACTCATTGCT
    AAAGTCATTC AGAATCTCGC CAATTTTGCT AAGTTTGGTA ACAAGGAAGA GTACATGGCT
    TTCATGAACG ACTTTCTGGA ACATGAGTGG GGGGGAATGA AGCGATTTCT CCTGGAGATC
    TCTAATCCAG ATACCATCTC AAACATGCCT GGATTTGAGG GCTATATCGA CCTGGGCAGA
    GAGCTCTCAG TTTTGCATTC CCTCTTATGG GAGGTTGTGT CCCAACTTGA TAAGGGTGAA
    AATTCCTTCC TACAGGCGAC CGTGGCAAAA CTGGGACCTC TTCCTCGTAT TCTTGCCGAC
    ATCACCAAAT CAATGACCAA CCCTACGCCA ATACAGCAGC AGCTGAGGCG TTTCACTGAG
    CACAGCTCTA GTCCAAACGT CAGTGGCAGT CTCTCCTCAG GGCTTCAGAA GATATTCGAG
    GACCCCACTG ATGGTGACTT GCATAAACTG AAGTCTCCAA CCCAGGAAAA CATAGATGGC
    TATTTCAGGG GCAAGACCTT GCTGTTGGTT CAGCAGGCGT CCACCCAGAG CATGACTTAC
    TCAGATAAGG ACGAAAGAGA GAGCATCTTG CCCAACGGAC GTAGCATCTC CCTCATGGAC
    CTGCAGGATC CCCATACCGC TCACAGCGAC CACGCTTCCA TGATGCACGA CGTGCCTTTG
    CGCTTGGCCG GCAGCCAGCT CTCCATCACC CAGGTGGCCA ACATCAAGCA GCTGTGGGAG
    ACCCAGAGCA CGCCCCAAAG CGCTCCCCAG GTGAGGAGGC CGCTGCACCC GGCTTTGAAC
    CAGCAGGGCA GCCTCCAGCC TCTGTCATTC CAGAACCCGG TGTACCACCT CAACAACCCA
    CCTGCGCCGT TGCCCAAGGC CTCCGTGGAC TCCAGCCTGG AGAACCTGAG CACCGCCAGC
    TCCCAGAGCC GAAGCAACAG CGAAGACTTC AAGCTCAGCG GGCCCAGCAA CAGCAGCATG
    GAGGACTTCA CCAAGCGCAG CACGCAGAGC GAGGACTTCT CTAGGCGGCA CACGGTCCCG
    GACAAGCACG TCCCCATCGC GTTGCCCCGC CAGAACAGCA GCAGCCAGGC ACAGATCCGC
    AAAATGGACC AGGCCGGGTT AGGGGCCAGG GCCAAAGCGC CGCAGTCGCT GCCGCACAGC
    GCGTCCCTGC GCAGCACGGG CAGCATGTCG GCCGTGTCGG GGGCCGTGGT GACTGAGCCC
    GTCCAGAACG GCAGCCGCTC CCGGCAGCAG TCGTCCTCCT CCAGGGAGAG CCCTGTGCCG
    AAGGTGAGGG CCATTCAGAG GCAGCAAACG CAGCAGGTCC AGTCTCCTGT GGACTCAGCC
    ACAATGTCAC CAGTGGAAAG GACAGCAGCG TGGGTTCTCA ATAACGGGCA GTACGAGGAG
    GTCGAGGAGG ACACAGAGCA GAACCCAGAT GAAGCCAAGC ATGCTGAGAA GTACGAACAA
    GAGATAGCAA AGCTGAAGGA ACGCTTGAAA GTGTCGAGCC GCCGGCTGGA GGAGTACGAG
    CGGCGGCTCC TGGTGCAGGA GCAGCAAATG CAGAAGCTGC TGATGGAGTA CAAGTCCAGG
    CTGGAGGACA GCGAGGAGAG ACTGCGCAGG CAACAGGAGG AGAAGGACAG CCAGATGAAA
    AGCATTATCA GCAGGCTCAT GGCAGTTGAA GAGGAACTGA AGAAAGATCA TGCAGAGATG
    CAAGCTGTCA TCGATGCAAA GCAGAAAATT ATTGATGCAC AGGAGAAGCG GATCGTGTCC
    CTGGACTCTG CCAACACGCG GCTGATGAGT GCCCTGACGC AGGTGAAGGA GCGCTACAGC
    ATGCAAGTCC GCAATGGCAT CTCCCCCACC AACCCCACCA AGCTTTCCAT CACGGAGAAT
    GGTGAATTCA AAAACAGCAG CTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004943302.2
    CDS632..4918
    Translation

    Target ORF information:

    RefSeq Version XM_004943245.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004943245.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAGTGCTT TTAAAAAGTA TTACAGTCTC TCTCCCAGGA CCTTTAGGAG GTCTTTGTCG 
    GAGCAAGATG CTGGCACCGC TGCCTTAAAG GACTATAATT GGTACCACCA AATGAGAAGC
    AAGAAGGGGA GCAAACAAAC AGCTTTGGAC CGAGAGAAAG CACTCGGAGC GTACAGGTGG
    CAAACACATC CCCGTCTTCC CTGGAGGACA AGCAGAAGAG CACACTCTTG CAGCCTCAGA
    GGGGAAGGTA ATGATTCGAG TCTTGTCAAA AGTTCTGCAA GCATGGATGC TACCAGCGCC
    GACGATCTCC ACCAAGACTC AACGGAGATG CTCTTCCAGG CAAGGACCAG ATCACCGCGG
    GTTCCCTTCA ACCGCTCCCT GTCTGAACCT GTGCCGCACT CTGCAAACAG GAATGCCAAG
    CCCTTTCTGC AGGCGGTGCG CTCTGTGGAT TGTGAGCAGC ACAGCTATTT TGTCCGTGGC
    ATCTTCTCAA CTCTCTCAAG TGGCTTTTCA AAGATAATAA ATCGAAGGGG TGAGCAGCCC
    CCCACTGAGC CACTGGAGGA GTGGAAACCA GCCGGTAGTC AAATTGACCC GTGCCCAGGT
    AATGGACTCT GTTCTGTTTG TGTTACAGAT GTAAAAGGAC CACCTACGCA CAGGCTGTCC
    TGCGGCCAGT CCCCCTACAC CGAGACGACA ACATGGGAGA GGAAGTACTG CATCCTGACT
    GACAGCCAGC TGGTGCTGCT CAACAGGGAG AAGGAGGTGC CCACCGAGGG GTTGCAGGAG
    CCGCAGGATG CAACCAAAGG CCGCTGTCTG CGCCGCACGG TGAGCGTGCC CTCCGAGGGC
    CAGTTCCCCG AGTACCCACC AGATGGGGCC GCCAAGCTGG AGGTCCCGGC AGAGAGATCT
    CCTCGCAGAC GGAGCATCTC TGGGACCAGC ACATCTGAGA AAACCAACTC CATGGATGCT
    GCAAATACCT CTCCTTTCAA AGTATCGGGC TTCTTCAGTA AACGTCTGAA AGGGTCCATC
    AAGAGGACAA AGAGTCAGTC AAAGCTGGAC AGGAACACAA GTTTCCGCCT CCCATCCCTC
    CGCAATGCTG ATGACAGGTC ACGTGGGCTG CCGAAGCTGA AGGAGTCCCG CTCTCATGAG
    TCCCTGCTGA GCCCCGGCAG CGCAGTGGAA GCCCTGGACC TTGGGGCAGA AGAAAAGGTC
    TTTGTAAAGC CACTGCACAG CAGCATCCTC GGACAGGACT TCTGCTTTGA GGTAACCTAC
    TCCAGCGGCA GTAAATGCTT CAGCTGTTCC TCTGCTGCAG AGAGGGATAA GTGGATGGAA
    AACCTACGCA GAACAGTGCA GCCGAATAAG GACAACTGCC GTCGAGCTGA AAATGTGCTC
    CGTCTCTGGA TCATTGAGGC AAAGGACCTG GCCCCCAAGA AGAAATACTT CTGTGAGCTG
    TGCCTTGATG ACACTCTCTT TGCCCGCACC ACCAGCAAAA CGAAAGCAGA TAACATTTTC
    TGGGGGGAGC ACTTTGAGTT CTACGGCCTC CCAGCTCTTC ACAGCATCAC GGTTCACATC
    TACAAGGATG TGGAGAAGAA AAAGAAAAAG GACAAAAACA ATTACGTCGG CCTGGTCAAC
    ATTCCCATGG CCACTGTAAC GGGGCGCCAG TTTGTGGAAA AATGGTACCC AGTCAGCACA
    CCTACACCCA ACAAAGGGAA ATCAGGAGGC CCTTCCATTC GCATCAAGTC GCGTTATCAG
    ACCATCACCA TCTTACCCAT GGAGCAATAC AAGGAGTTTG CAGAGTTTGT TACCAGCAAC
    TACACGATGC TGTGCTCTGT GCTGGAGCCA GTCATCAGCG TAAGGAATAA GGAAGAGATG
    GCTTGTGCAA TGGTGCACAT TCTTCAGAGC ACGGGGAGAG CCAAGGACTT CCTAACGGAT
    CTGGTGATGT CTGAGGTGGA TCGCTGCGGG GAACACGATG TCTTGATCTT CAGGGAGAAC
    ACACTTGCTA CCAAAGCTAT TGAGGAATAC CTGAAGTTGG TAGGGCAGAA GTATCTCCAT
    GATGCGCTAG GGGAGTTTAT CAAGGCTTTG TATGAGTCAG ATGAAAACTG TGAAGTGGAC
    CCCAGCAAAT GTTCATCCAG TGAATTAACA GACCATCAGA GCAACCTGAA AATGTGTTGT
    GAGCTGGCCT TCTGCAAGAT TATCAATTCC TACTGCGTGT TCCCTCGTGA GCTGAAGGAG
    GTGTTTGCAT CTTGGAAGCA GCAGTGCCTG AGCAGGGGCA AGCAGGACAT CAGCGAGCGG
    CTGATCAGTG CCTCCCTGTT CCTCCGCTTC CTGTGCCCCG CCATCATGTC CCCCAGCCTC
    TTCAGCCTCA TGCAGGAGTA TCCTGACGAC CGGACATCGC GCACGCTCAC ACTCATTGCT
    AAAGTCATTC AGAATCTCGC CAATTTTGCT AAGTTTGGTA ACAAGGAAGA GTACATGGCT
    TTCATGAACG ACTTTCTGGA ACATGAGTGG GGGGGAATGA AGCGATTTCT CCTGGAGATC
    TCTAATCCAG ATACCATCTC AAACATGCCT GGATTTGAGG GCTATATCGA CCTGGGCAGA
    GAGCTCTCAG TTTTGCATTC CCTCTTATGG GAGGTTGTGT CCCAACTTGA TAAGGGTGAA
    AATTCCTTCC TACAGGCGAC CGTGGCAAAA CTGGGACCTC TTCCTCGTAT TCTTGCCGAC
    ATCACCAAAT CAATGACCAA CCCTACGCCA ATACAGCAGC AGCTGAGGCG TTTCACTGAG
    CACAGCTCTA GTCCAAACGT CAGTGGCAGT CTCTCCTCAG GGCTTCAGAA GATATTCGAG
    GACCCCACTG ATGGTGACTT GCATAAACTG AAGTCTCCAA CCCAGGAAAA CATAGATGGC
    TATTTCAGGG GCAAGACCTT GCTGTTGGTT CAGCAGGCGT CCACCCAGAG CATGACTTAC
    TCAGATAAGG ACGAAAGAGA GAGCATCTTG CCCAACGGAC GTAGCATCTC CCTCATGGAC
    CTGCAGGATC CCCATACCGC TCACAGCGAC CACGCTTCCA TGATGCACGA CGTGCCTTTG
    CGCTTGGCCG GCAGCCAGCT CTCCATCACC CAGGTGGCCA ACATCAAGCA GCTGTGGGAG
    ACCCAGAGCA CGCCCCAAAG CGCTCCCCAG GTGAGGAGGC CGCTGCACCC GGCTTTGAAC
    CAGCAGGGCA GCCTCCAGCC TCTGTCATTC CAGAACCCGG TGTACCACCT CAACAACCCA
    CCTGCGCCGT TGCCCAAGGC CTCCGTGGAC TCCAGCCTGG AGAACCTGAG CACCGCCAGC
    TCCCAGAGCC GAAGCAACAG CGAAGACTTC AAGCTCAGCG GGCCCAGCAA CAGCAGCATG
    GAGGACTTCA CCAAGCGCAG CACGCAGAGC GAGGACTTCT CTAGGCGGCA CACGGTCCCG
    GACAAGCACG TCCCCATCGC GTTGCCCCGC CAGAACAGCA GCAGCCAGGC ACAGATCCGC
    AAAATGGACC AGGCCGGGTT AGGGGCCAGG GCCAAAGCGC CGCAGTCGCT GCCGCACAGC
    GCGTCCCTGC GCAGCACGGG CAGCATGTCG GCCGTGTCGG GGGCCGTGGT GACTGAGCCC
    GTCCAGAACG GCAGCCGCTC CCGGCAGCAG TCGTCCTCCT CCAGGGAGAG CCCTGTGCCG
    AAGGTGAGGG CCATTCAGAG GCAGCAAACG CAGCAGGTCC AGTCTCCTGT GGACTCAGCC
    ACAATGTCAC CAGTGGAAAG GACAGCAGCG TGGGTTCTCA ATAACGGGCA GTACGAGGAG
    GTCGAGGAGG ACACAGAGCA GAACCCAGAT GAAGCCAAGC ATGCTGAGAA GTACGAACAA
    GAGATAGCAA AGCTGAAGGA ACGCTTGAAA GTGTCGAGCC GCCGGCTGGA GGAGTACGAG
    CGGCGGCTCC TGGTGCAGGA GCAGCAAATG CAGAAGCTGC TGATGGAGTA CAAGTCCAGG
    CTGGAGGACA GCGAGGAGAG ACTGCGCAGG CAACAGGAGG AGAAGGACAG CCAGATGAAA
    AGCATTATCA GCAGGCTCAT GGCAGTTGAA GAGGAACTGA AGAAAGATCA TGCAGAGATG
    CAAGCTGTCA TCGATGCAAA GCAGAAAATT ATTGATGCAC AGGAGAAGCG GATCGTGTCC
    CTGGACTCTG CCAACACGCG GCTGATGAGT GCCCTGACGC AGGTGAAGGA GCGCTACAGC
    ATGCAAGTCC GCAATGGCAT CTCCCCCACC AACCCCACCA AGCTTTCCAT CACGGAGAAT
    GGTGAATTCA AAAACAGCAG CTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa152363
    Clone ID Related Accession (Same CDS sequence) XM_015290511.2
    Accession Version XM_015290511.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4275bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product ras GTPase-activating protein nGAP isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006095.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015290511.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAGTGCTT TTAAAAAGTA TTACAGTCTC TCTCCCAGGA CCTTTAGGAG GTCTTTGTCG 
    GAGCAAGATG CTGGCACCGC TGCCTTAAAG GACTATAATT GGTACCACCA AATGAGAAGC
    AAGAAGGGGA GCAAACAAAC AGCTTTGGAC CGAGAGAAAG CACTCGGAGC GTACAGGTGG
    CAAACACATC CCCGTCTTCC CTGGAGGACA AGCAGAAGAG CACACTCTTG CAGCCTCAGA
    GGGGAAGGTA ATGATTCGAG TCTTGTCAAA AGTTCTGCAA GCATGGATGC TACCAGCGCC
    GACGATCTCC ACCAAGACTC AACGGAGATG CTCTTCCAGG CAAGGACCAG ATCACCGCGG
    GTTCCCTTCA ACCGCTCCCT GTCTGAACCT GTGCCGCACT CTGCAAACAG GAATGCCAAG
    CCCTTTCTGC AGGCGGTGCG CTCTGTGGAT TGTGAGCAGC ACAGCTATTT TGTCCGTGGC
    ATCTTCTCAA CTCTCTCAAG TGGCTTTTCA AAGATAATAA ATCGAAGGGG TGAGCAGCCC
    CCCACTGAGC CACTGGAGGA GTGGAAACCA GCCGGTAGTC AAATTGACCC GTGCCCAGGT
    AATGGACTCT GTTCTGTTTG TGTTACAGAT GTAAAAGGAC CACCTACGCA CAGGCTGTCC
    TGCGGCCAGT CCCCCTACAC CGAGACGACA ACATGGGAGA GGAAGTACTG CATCCTGACT
    GACAGCCAGC TGGTGCTGCT CAACAGGGAG AAGGAGGTGC CCACCGAGGG GTTGCAGGAG
    CCGCAGGATG CAACCAAAGG CCGCTGTCTG CGCCGCACGG TGAGCGTGCC CTCCGAGGGC
    CAGTTCCCCG AGTACCCACC AGATGGGGCC GCCAAGCTGG AGGTCCCGGC AGAGAGATCT
    CCTCGCAGAC GGAGCATCTC TGGGACCAGC ACATCTGAGA AAACCAACTC CATGGATGCT
    GCAAATACCT CTCCTTTCAA AGTATCGGGC TTCTTCAGTA AACGTCTGAA AGGGTCCATC
    AAGAGGACAA AGAGTCAGTC AAAGCTGGAC AGGAACACAA GTTTCCGCCT CCCATCCCTC
    CGCAATGCTG ATGACAGGTC ACGTGGGCTG CCGAAGCTGA AGGAGTCCCG CTCTCATGAG
    TCCCTGCTGA GCCCCGGCAG CGCAGTGGAA GCCCTGGACC TTGGGGCAGA AGAAAAGGTC
    TTTGTAAAGC CACTGCACAG CAGCATCCTC GGACAGGACT TCTGCTTTGA GGTAACCTAC
    TCCAGCGGCA GTAAATGCTT CAGCTGTTCC TCTGCTGCAG AGAGGGATAA GTGGATGGAA
    AACCTACGCA GAACAGTGCA GCCGAATAAG GACAACTGCC GTCGAGCTGA AAATGTGCTC
    CGTCTCTGGA TCATTGAGGC AAAGGACCTG GCCCCCAAGA AGAAATACTT CTGTGAGCTG
    TGCCTTGATG ACACTCTCTT TGCCCGCACC ACCAGCAAAA CGAAAGCAGA TAACATTTTC
    TGGGGGGAGC ACTTTGAGTT CTACGGCCTC CCAGCTCTTC ACAGCATCAC GGTTCACATC
    TACAAGGATG TGGAGAAGAA AAAGAAAAAG GACAAAAACA ATTACGTCGG CCTGGTCAAC
    ATTCCCATGG CCACTGTAAC GGGGCGCCAG TTTGTGGAAA AATGGTACCC AGTCAGCACA
    CCTACACCCA ACAAAGGGAA ATCAGGAGGC CCTTCCATTC GCATCAAGTC GCGTTATCAG
    ACCATCACCA TCTTACCCAT GGAGCAATAC AAGGAGTTTG CAGAGTTTGT TACCAGCAAC
    TACACGATGC TGTGCTCTGT GCTGGAGCCA GTCATCAGCG TAAGGAATAA GGAAGAGATG
    GCTTGTGCAA TGGTGCACAT TCTTCAGAGC ACGGGGAGAG CCAAGGACTT CCTAACGGAT
    CTGGTGATGT CTGAGGTGGA TCGCTGCGGG GAACACGATG TCTTGATCTT CAGGGAGAAC
    ACACTTGCTA CCAAAGCTAT TGAGGAATAC CTGAAGTTGG TAGGGCAGAA GTATCTCCAT
    GATGCGCTAG GGGAGTTTAT CAAGGCTTTG TATGAGTCAG ATGAAAACTG TGAAGTGGAC
    CCCAGCAAAT GTTCATCCAG TGAATTAACA GACCATCAGA GCAACCTGAA AATGTGTTGT
    GAGCTGGCCT TCTGCAAGAT TATCAATTCC TACTGCGTGT TCCCTCGTGA GCTGAAGGAG
    GTGTTTGCAT CTTGGAAGCA GCAGTGCCTG AGCAGGGGCA AGCAGGACAT CAGCGAGCGG
    CTGATCAGTG CCTCCCTGTT CCTCCGCTTC CTGTGCCCCG CCATCATGTC CCCCAGCCTC
    TTCAGCCTCA TGCAGGAGTA TCCTGACGAC CGGACATCGC GCACGCTCAC ACTCATTGCT
    AAAGTCATTC AGAATCTCGC CAATTTTGCT AAGTTTGGTA ACAAGGAAGA GTACATGGCT
    TTCATGAACG ACTTTCTGGA ACATGAGTGG GGGGGAATGA AGCGATTTCT CCTGGAGATC
    TCTAATCCAG ATACCATCTC AAACATGCCT GGATTTGAGG GCTATATCGA CCTGGGCAGA
    GAGCTCTCAG TTTTGCATTC CCTCTTATGG GAGGTTGTGT CCCAACTTGA TAAGGCGACC
    GTGGCAAAAC TGGGACCTCT TCCTCGTATT CTTGCCGACA TCACCAAATC AATGACCAAC
    CCTACGCCAA TACAGCAGCA GCTGAGGCGT TTCACTGAGC ACAGCTCTAG TCCAAACGTC
    AGTGGCAGTC TCTCCTCAGG GCTTCAGAAG ATATTCGAGG ACCCCACTGA TGGTGACTTG
    CATAAACTGA AGTCTCCAAC CCAGGAAAAC ATAGATGGCT ATTTCAGGGG CAAGACCTTG
    CTGTTGGTTC AGCAGGCGTC CACCCAGAGC ATGACTTACT CAGATAAGGA CGAAAGAGAG
    AGCATCTTGC CCAACGGACG TAGCATCTCC CTCATGGACC TGCAGGATCC CCATACCGCT
    CACAGCGACC ACGCTTCCAT GATGCACGAC GTGCCTTTGC GCTTGGCCGG CAGCCAGCTC
    TCCATCACCC AGGTGGCCAA CATCAAGCAG CTGTGGGAGA CCCAGAGCAC GCCCCAAAGC
    GCTCCCCAGG TGAGGAGGCC GCTGCACCCG GCTTTGAACC AGCAGGGCAG CCTCCAGCCT
    CTGTCATTCC AGAACCCGGT GTACCACCTC AACAACCCAC CTGCGCCGTT GCCCAAGGCC
    TCCGTGGACT CCAGCCTGGA GAACCTGAGC ACCGCCAGCT CCCAGAGCCG AAGCAACAGC
    GAAGACTTCA AGCTCAGCGG GCCCAGCAAC AGCAGCATGG AGGACTTCAC CAAGCGCAGC
    ACGCAGAGCG AGGACTTCTC TAGGCGGCAC ACGGTCCCGG ACAAGCACGT CCCCATCGCG
    TTGCCCCGCC AGAACAGCAG CAGCCAGGCA CAGATCCGCA AAATGGACCA GGCCGGGTTA
    GGGGCCAGGG CCAAAGCGCC GCAGTCGCTG CCGCACAGCG CGTCCCTGCG CAGCACGGGC
    AGCATGTCGG CCGTGTCGGG GGCCGTGGTG ACTGAGCCCG TCCAGAACGG CAGCCGCTCC
    CGGCAGCAGT CGTCCTCCTC CAGGGAGAGC CCTGTGCCGA AGGTGAGGGC CATTCAGAGG
    CAGCAAACGC AGCAGGTCCA GTCTCCTGTG GACTCAGCCA CAATGTCACC AGTGGAAAGG
    ACAGCAGCGT GGGTTCTCAA TAACGGGCAG TACGAGGAGG TCGAGGAGGA CACAGAGCAG
    AACCCAGATG AAGCCAAGCA TGCTGAGAAG TACGAACAAG AGATAGCAAA GCTGAAGGAA
    CGCTTGAAAG TGTCGAGCCG CCGGCTGGAG GAGTACGAGC GGCGGCTCCT GGTGCAGGAG
    CAGCAAATGC AGAAGCTGCT GATGGAGTAC AAGTCCAGGC TGGAGGACAG CGAGGAGAGA
    CTGCGCAGGC AACAGGAGGA GAAGGACAGC CAGATGAAAA GCATTATCAG CAGGCTCATG
    GCAGTTGAAG AGGAACTGAA GAAAGATCAT GCAGAGATGC AAGCTGTCAT CGATGCAAAG
    CAGAAAATTA TTGATGCACA GGTCATTAAT GGGAATGAGA TAATTCAAAC AGGAGAAGCG
    GATCGTGTCC CTGGACTCTG CCAACACGCG GCTGATGAGT GCCCTGACGC AGGTGAAGGA
    GCGCTACAGC ATGCAAGTCC GCAATGGCAT CTCCCCCACC AACCCCACCA AGCTTTCCAT
    CACGGAGAAT GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015145997.1
    CDS637..4911
    Translation

    Target ORF information:

    RefSeq Version XM_015290511.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015290511.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAGTGCTT TTAAAAAGTA TTACAGTCTC TCTCCCAGGA CCTTTAGGAG GTCTTTGTCG 
    GAGCAAGATG CTGGCACCGC TGCCTTAAAG GACTATAATT GGTACCACCA AATGAGAAGC
    AAGAAGGGGA GCAAACAAAC AGCTTTGGAC CGAGAGAAAG CACTCGGAGC GTACAGGTGG
    CAAACACATC CCCGTCTTCC CTGGAGGACA AGCAGAAGAG CACACTCTTG CAGCCTCAGA
    GGGGAAGGTA ATGATTCGAG TCTTGTCAAA AGTTCTGCAA GCATGGATGC TACCAGCGCC
    GACGATCTCC ACCAAGACTC AACGGAGATG CTCTTCCAGG CAAGGACCAG ATCACCGCGG
    GTTCCCTTCA ACCGCTCCCT GTCTGAACCT GTGCCGCACT CTGCAAACAG GAATGCCAAG
    CCCTTTCTGC AGGCGGTGCG CTCTGTGGAT TGTGAGCAGC ACAGCTATTT TGTCCGTGGC
    ATCTTCTCAA CTCTCTCAAG TGGCTTTTCA AAGATAATAA ATCGAAGGGG TGAGCAGCCC
    CCCACTGAGC CACTGGAGGA GTGGAAACCA GCCGGTAGTC AAATTGACCC GTGCCCAGGT
    AATGGACTCT GTTCTGTTTG TGTTACAGAT GTAAAAGGAC CACCTACGCA CAGGCTGTCC
    TGCGGCCAGT CCCCCTACAC CGAGACGACA ACATGGGAGA GGAAGTACTG CATCCTGACT
    GACAGCCAGC TGGTGCTGCT CAACAGGGAG AAGGAGGTGC CCACCGAGGG GTTGCAGGAG
    CCGCAGGATG CAACCAAAGG CCGCTGTCTG CGCCGCACGG TGAGCGTGCC CTCCGAGGGC
    CAGTTCCCCG AGTACCCACC AGATGGGGCC GCCAAGCTGG AGGTCCCGGC AGAGAGATCT
    CCTCGCAGAC GGAGCATCTC TGGGACCAGC ACATCTGAGA AAACCAACTC CATGGATGCT
    GCAAATACCT CTCCTTTCAA AGTATCGGGC TTCTTCAGTA AACGTCTGAA AGGGTCCATC
    AAGAGGACAA AGAGTCAGTC AAAGCTGGAC AGGAACACAA GTTTCCGCCT CCCATCCCTC
    CGCAATGCTG ATGACAGGTC ACGTGGGCTG CCGAAGCTGA AGGAGTCCCG CTCTCATGAG
    TCCCTGCTGA GCCCCGGCAG CGCAGTGGAA GCCCTGGACC TTGGGGCAGA AGAAAAGGTC
    TTTGTAAAGC CACTGCACAG CAGCATCCTC GGACAGGACT TCTGCTTTGA GGTAACCTAC
    TCCAGCGGCA GTAAATGCTT CAGCTGTTCC TCTGCTGCAG AGAGGGATAA GTGGATGGAA
    AACCTACGCA GAACAGTGCA GCCGAATAAG GACAACTGCC GTCGAGCTGA AAATGTGCTC
    CGTCTCTGGA TCATTGAGGC AAAGGACCTG GCCCCCAAGA AGAAATACTT CTGTGAGCTG
    TGCCTTGATG ACACTCTCTT TGCCCGCACC ACCAGCAAAA CGAAAGCAGA TAACATTTTC
    TGGGGGGAGC ACTTTGAGTT CTACGGCCTC CCAGCTCTTC ACAGCATCAC GGTTCACATC
    TACAAGGATG TGGAGAAGAA AAAGAAAAAG GACAAAAACA ATTACGTCGG CCTGGTCAAC
    ATTCCCATGG CCACTGTAAC GGGGCGCCAG TTTGTGGAAA AATGGTACCC AGTCAGCACA
    CCTACACCCA ACAAAGGGAA ATCAGGAGGC CCTTCCATTC GCATCAAGTC GCGTTATCAG
    ACCATCACCA TCTTACCCAT GGAGCAATAC AAGGAGTTTG CAGAGTTTGT TACCAGCAAC
    TACACGATGC TGTGCTCTGT GCTGGAGCCA GTCATCAGCG TAAGGAATAA GGAAGAGATG
    GCTTGTGCAA TGGTGCACAT TCTTCAGAGC ACGGGGAGAG CCAAGGACTT CCTAACGGAT
    CTGGTGATGT CTGAGGTGGA TCGCTGCGGG GAACACGATG TCTTGATCTT CAGGGAGAAC
    ACACTTGCTA CCAAAGCTAT TGAGGAATAC CTGAAGTTGG TAGGGCAGAA GTATCTCCAT
    GATGCGCTAG GGGAGTTTAT CAAGGCTTTG TATGAGTCAG ATGAAAACTG TGAAGTGGAC
    CCCAGCAAAT GTTCATCCAG TGAATTAACA GACCATCAGA GCAACCTGAA AATGTGTTGT
    GAGCTGGCCT TCTGCAAGAT TATCAATTCC TACTGCGTGT TCCCTCGTGA GCTGAAGGAG
    GTGTTTGCAT CTTGGAAGCA GCAGTGCCTG AGCAGGGGCA AGCAGGACAT CAGCGAGCGG
    CTGATCAGTG CCTCCCTGTT CCTCCGCTTC CTGTGCCCCG CCATCATGTC CCCCAGCCTC
    TTCAGCCTCA TGCAGGAGTA TCCTGACGAC CGGACATCGC GCACGCTCAC ACTCATTGCT
    AAAGTCATTC AGAATCTCGC CAATTTTGCT AAGTTTGGTA ACAAGGAAGA GTACATGGCT
    TTCATGAACG ACTTTCTGGA ACATGAGTGG GGGGGAATGA AGCGATTTCT CCTGGAGATC
    TCTAATCCAG ATACCATCTC AAACATGCCT GGATTTGAGG GCTATATCGA CCTGGGCAGA
    GAGCTCTCAG TTTTGCATTC CCTCTTATGG GAGGTTGTGT CCCAACTTGA TAAGGCGACC
    GTGGCAAAAC TGGGACCTCT TCCTCGTATT CTTGCCGACA TCACCAAATC AATGACCAAC
    CCTACGCCAA TACAGCAGCA GCTGAGGCGT TTCACTGAGC ACAGCTCTAG TCCAAACGTC
    AGTGGCAGTC TCTCCTCAGG GCTTCAGAAG ATATTCGAGG ACCCCACTGA TGGTGACTTG
    CATAAACTGA AGTCTCCAAC CCAGGAAAAC ATAGATGGCT ATTTCAGGGG CAAGACCTTG
    CTGTTGGTTC AGCAGGCGTC CACCCAGAGC ATGACTTACT CAGATAAGGA CGAAAGAGAG
    AGCATCTTGC CCAACGGACG TAGCATCTCC CTCATGGACC TGCAGGATCC CCATACCGCT
    CACAGCGACC ACGCTTCCAT GATGCACGAC GTGCCTTTGC GCTTGGCCGG CAGCCAGCTC
    TCCATCACCC AGGTGGCCAA CATCAAGCAG CTGTGGGAGA CCCAGAGCAC GCCCCAAAGC
    GCTCCCCAGG TGAGGAGGCC GCTGCACCCG GCTTTGAACC AGCAGGGCAG CCTCCAGCCT
    CTGTCATTCC AGAACCCGGT GTACCACCTC AACAACCCAC CTGCGCCGTT GCCCAAGGCC
    TCCGTGGACT CCAGCCTGGA GAACCTGAGC ACCGCCAGCT CCCAGAGCCG AAGCAACAGC
    GAAGACTTCA AGCTCAGCGG GCCCAGCAAC AGCAGCATGG AGGACTTCAC CAAGCGCAGC
    ACGCAGAGCG AGGACTTCTC TAGGCGGCAC ACGGTCCCGG ACAAGCACGT CCCCATCGCG
    TTGCCCCGCC AGAACAGCAG CAGCCAGGCA CAGATCCGCA AAATGGACCA GGCCGGGTTA
    GGGGCCAGGG CCAAAGCGCC GCAGTCGCTG CCGCACAGCG CGTCCCTGCG CAGCACGGGC
    AGCATGTCGG CCGTGTCGGG GGCCGTGGTG ACTGAGCCCG TCCAGAACGG CAGCCGCTCC
    CGGCAGCAGT CGTCCTCCTC CAGGGAGAGC CCTGTGCCGA AGGTGAGGGC CATTCAGAGG
    CAGCAAACGC AGCAGGTCCA GTCTCCTGTG GACTCAGCCA CAATGTCACC AGTGGAAAGG
    ACAGCAGCGT GGGTTCTCAA TAACGGGCAG TACGAGGAGG TCGAGGAGGA CACAGAGCAG
    AACCCAGATG AAGCCAAGCA TGCTGAGAAG TACGAACAAG AGATAGCAAA GCTGAAGGAA
    CGCTTGAAAG TGTCGAGCCG CCGGCTGGAG GAGTACGAGC GGCGGCTCCT GGTGCAGGAG
    CAGCAAATGC AGAAGCTGCT GATGGAGTAC AAGTCCAGGC TGGAGGACAG CGAGGAGAGA
    CTGCGCAGGC AACAGGAGGA GAAGGACAGC CAGATGAAAA GCATTATCAG CAGGCTCATG
    GCAGTTGAAG AGGAACTGAA GAAAGATCAT GCAGAGATGC AAGCTGTCAT CGATGCAAAG
    CAGAAAATTA TTGATGCACA GGTCATTAAT GGGAATGAGA TAATTCAAAC AGGAGAAGCG
    GATCGTGTCC CTGGACTCTG CCAACACGCG GCTGATGAGT GCCCTGACGC AGGTGAAGGA
    GCGCTACAGC ATGCAAGTCC GCAATGGCAT CTCCCCCACC AACCCCACCA AGCTTTCCAT
    CACGGAGAAT GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa152364
    Clone ID Related Accession (Same CDS sequence) XM_004943246.3
    Accession Version XM_004943246.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4266bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product ras GTPase-activating protein nGAP isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006095.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004943246.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAGTGCTT TTAAAAAGTA TTACAGTCTC TCTCCCAGGA CCTTTAGGAG GTCTTTGTCG 
    GAGCAAGATG CTGGCACCGC TGCCTTAAAG GACTATAATT GGTACCACCA AATGAGAAGC
    AAGAAGGGGA GCAAACAAAC AGCTTTGGAC CGAGAGAAAG CACTCGGAGC GTACAGGTGG
    CAAACACATC CCCGTCTTCC CTGGAGGACA AGCAGAAGAG CACACTCTTG CAGCCTCAGA
    GGGGAAGGTA ATGATTCGAG TCTTGTCAAA AGTTCTGCAA GCATGGATGC TACCAGCGCC
    GACGATCTCC ACCAAGACTC AACGGAGATG CTCTTCCAGG CAAGGACCAG ATCACCGCGG
    GTTCCCTTCA ACCGCTCCCT GTCTGAACCT GTGCCGCACT CTGCAAACAG GAATGCCAAG
    CCCTTTCTGC AGGCGGTGCG CTCTGTGGAT TGTGAGCAGC ACAGCTATTT TGTCCGTGGC
    ATCTTCTCAA CTCTCTCAAG TGGCTTTTCA AAGATAATAA ATCGAAGGGG TGAGCAGCCC
    CCCACTGAGC CACTGGAGGA GTGGAAACCA GCCGGTAGTC AAATTGACCC GTGCCCAGGT
    AATGGACTCT GTTCTGTTTG TGTTACAGAT GTAAAAGGAC CACCTACGCA CAGGCTGTCC
    TGCGGCCAGT CCCCCTACAC CGAGACGACA ACATGGGAGA GGAAGTACTG CATCCTGACT
    GACAGCCAGC TGGTGCTGCT CAACAGGGAG AAGGAGGTGC CCACCGAGGG GTTGCAGGAG
    CCGCAGGATG CAACCAAAGG CCGCTGTCTG CGCCGCACGG TGAGCGTGCC CTCCGAGGGC
    CAGTTCCCCG AGTACCCACC AGATGGGGCC GCCAAGCTGG AGGTCCCGGC AGAGAGATCT
    CCTCGCAGAC GGAGCATCTC TGGGACCAGC ACATCTGAGA AAACCAACTC CATGGATGCT
    GCAAATACCT CTCCTTTCAA AGTATCGGGC TTCTTCAGTA AACGTCTGAA AGGGTCCATC
    AAGAGGACAA AGAGTCAGTC AAAGCTGGAC AGGAACACAA GTTTCCGCCT CCCATCCCTC
    CGCAATGCTG ATGACAGGTC ACGTGGGCTG CCGAAGCTGA AGGAGTCCCG CTCTCATGAG
    TCCCTGCTGA GCCCCGGCAG CGCAGTGGAA GCCCTGGACC TTGGGGCAGA AGAAAAGGTC
    TTTGTAAAGC CACTGCACAG CAGCATCCTC GGACAGGACT TCTGCTTTGA GGTAACCTAC
    TCCAGCGGCA GTAAATGCTT CAGCTGTTCC TCTGCTGCAG AGAGGGATAA GTGGATGGAA
    AACCTACGCA GAACAGTGCA GCCGAATAAG GACAACTGCC GTCGAGCTGA AAATGTGCTC
    CGTCTCTGGA TCATTGAGGC AAAGGACCTG GCCCCCAAGA AGAAATACTT CTGTGAGCTG
    TGCCTTGATG ACACTCTCTT TGCCCGCACC ACCAGCAAAA CGAAAGCAGA TAACATTTTC
    TGGGGGGAGC ACTTTGAGTT CTACGGCCTC CCAGCTCTTC ACAGCATCAC GGTTCACATC
    TACAAGGATG TGGAGAAGAA AAAGAAAAAG GACAAAAACA ATTACGTCGG CCTGGTCAAC
    ATTCCCATGG CCACTGTAAC GGGGCGCCAG TTTGTGGAAA AATGGTACCC AGTCAGCACA
    CCTACACCCA ACAAAGGGAA ATCAGGAGGC CCTTCCATTC GCATCAAGTC GCGTTATCAG
    ACCATCACCA TCTTACCCAT GGAGCAATAC AAGGAGTTTG CAGAGTTTGT TACCAGCAAC
    TACACGATGC TGTGCTCTGT GCTGGAGCCA GTCATCAGCG TAAGGAATAA GGAAGAGATG
    GCTTGTGCAA TGGTGCACAT TCTTCAGAGC ACGGGGAGAG CCAAGGACTT CCTAACGGAT
    CTGGTGATGT CTGAGGTGGA TCGCTGCGGG GAACACGATG TCTTGATCTT CAGGGAGAAC
    ACACTTGCTA CCAAAGCTAT TGAGGAATAC CTGAAGTTGG TAGGGCAGAA GTATCTCCAT
    GATGCGCTAG GGGAGTTTAT CAAGGCTTTG TATGAGTCAG ATGAAAACTG TGAAGTGGAC
    CCCAGCAAAT GTTCATCCAG TGAATTAACA GACCATCAGA GCAACCTGAA AATGTGTTGT
    GAGCTGGCCT TCTGCAAGAT TATCAATTCC TACTGCGTGT TCCCTCGTGA GCTGAAGGAG
    GTGTTTGCAT CTTGGAAGCA GCAGTGCCTG AGCAGGGGCA AGCAGGACAT CAGCGAGCGG
    CTGATCAGTG CCTCCCTGTT CCTCCGCTTC CTGTGCCCCG CCATCATGTC CCCCAGCCTC
    TTCAGCCTCA TGCAGGAGTA TCCTGACGAC CGGACATCGC GCACGCTCAC ACTCATTGCT
    AAAGTCATTC AGAATCTCGC CAATTTTGCT AAGTTTGGTA ACAAGGAAGA GTACATGGCT
    TTCATGAACG ACTTTCTGGA ACATGAGTGG GGGGGAATGA AGCGATTTCT CCTGGAGATC
    TCTAATCCAG ATACCATCTC AAACATGCCT GGATTTGAGG GCTATATCGA CCTGGGCAGA
    GAGCTCTCAG TTTTGCATTC CCTCTTATGG GAGGTTGTGT CCCAACTTGA TAAGGCGACC
    GTGGCAAAAC TGGGACCTCT TCCTCGTATT CTTGCCGACA TCACCAAATC AATGACCAAC
    CCTACGCCAA TACAGCAGCA GCTGAGGCGT TTCACTGAGC ACAGCTCTAG TCCAAACGTC
    AGTGGCAGTC TCTCCTCAGG GCTTCAGAAG ATATTCGAGG ACCCCACTGA TGGTGACTTG
    CATAAACTGA AGTCTCCAAC CCAGGAAAAC ATAGATGGCT ATTTCAGGGG CAAGACCTTG
    CTGTTGGTTC AGCAGGCGTC CACCCAGAGC ATGACTTACT CAGATAAGGA CGAAAGAGAG
    AGCATCTTGC CCAACGGACG TAGCATCTCC CTCATGGACC TGCAGGATCC CCATACCGCT
    CACAGCGACC ACGCTTCCAT GATGCACGAC GTGCCTTTGC GCTTGGCCGG CAGCCAGCTC
    TCCATCACCC AGGTGGCCAA CATCAAGCAG CTGTGGGAGA CCCAGAGCAC GCCCCAAAGC
    GCTCCCCAGG TGAGGAGGCC GCTGCACCCG GCTTTGAACC AGCAGGGCAG CCTCCAGCCT
    CTGTCATTCC AGAACCCGGT GTACCACCTC AACAACCCAC CTGCGCCGTT GCCCAAGGCC
    TCCGTGGACT CCAGCCTGGA GAACCTGAGC ACCGCCAGCT CCCAGAGCCG AAGCAACAGC
    GAAGACTTCA AGCTCAGCGG GCCCAGCAAC AGCAGCATGG AGGACTTCAC CAAGCGCAGC
    ACGCAGAGCG AGGACTTCTC TAGGCGGCAC ACGGTCCCGG ACAAGCACGT CCCCATCGCG
    TTGCCCCGCC AGAACAGCAG CAGCCAGGCA CAGATCCGCA AAATGGACCA GGCCGGGTTA
    GGGGCCAGGG CCAAAGCGCC GCAGTCGCTG CCGCACAGCG CGTCCCTGCG CAGCACGGGC
    AGCATGTCGG CCGTGTCGGG GGCCGTGGTG ACTGAGCCCG TCCAGAACGG CAGCCGCTCC
    CGGCAGCAGT CGTCCTCCTC CAGGGAGAGC CCTGTGCCGA AGGTGAGGGC CATTCAGAGG
    CAGCAAACGC AGCAGGTCCA GTCTCCTGTG GACTCAGCCA CAATGTCACC AGTGGAAAGG
    ACAGCAGCGT GGGTTCTCAA TAACGGGCAG TACGAGGAGG TCGAGGAGGA CACAGAGCAG
    AACCCAGATG AAGCCAAGCA TGCTGAGAAG TACGAACAAG AGATAGCAAA GCTGAAGGAA
    CGCTTGAAAG TGTCGAGCCG CCGGCTGGAG GAGTACGAGC GGCGGCTCCT GGTGCAGGAG
    CAGCAAATGC AGAAGCTGCT GATGGAGTAC AAGTCCAGGC TGGAGGACAG CGAGGAGAGA
    CTGCGCAGGC AACAGGAGGA GAAGGACAGC CAGATGAAAA GCATTATCAG CAGGCTCATG
    GCAGTTGAAG AGGAACTGAA GAAAGATCAT GCAGAGATGC AAGCTGTCAT CGATGCAAAG
    CAGAAAATTA TTGATGCACA GGAGAAGCGG ATCGTGTCCC TGGACTCTGC CAACACGCGG
    CTGATGAGTG CCCTGACGCA GGTGAAGGAG CGCTACAGCA TGCAAGTCCG CAATGGCATC
    TCCCCCACCA ACCCCACCAA GCTTTCCATC ACGGAGAATG GTGAATTCAA AAACAGCAGC
    TGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004943303.2
    CDS632..4897
    Translation

    Target ORF information:

    RefSeq Version XM_004943246.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004943246.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAGTGCTT TTAAAAAGTA TTACAGTCTC TCTCCCAGGA CCTTTAGGAG GTCTTTGTCG 
    GAGCAAGATG CTGGCACCGC TGCCTTAAAG GACTATAATT GGTACCACCA AATGAGAAGC
    AAGAAGGGGA GCAAACAAAC AGCTTTGGAC CGAGAGAAAG CACTCGGAGC GTACAGGTGG
    CAAACACATC CCCGTCTTCC CTGGAGGACA AGCAGAAGAG CACACTCTTG CAGCCTCAGA
    GGGGAAGGTA ATGATTCGAG TCTTGTCAAA AGTTCTGCAA GCATGGATGC TACCAGCGCC
    GACGATCTCC ACCAAGACTC AACGGAGATG CTCTTCCAGG CAAGGACCAG ATCACCGCGG
    GTTCCCTTCA ACCGCTCCCT GTCTGAACCT GTGCCGCACT CTGCAAACAG GAATGCCAAG
    CCCTTTCTGC AGGCGGTGCG CTCTGTGGAT TGTGAGCAGC ACAGCTATTT TGTCCGTGGC
    ATCTTCTCAA CTCTCTCAAG TGGCTTTTCA AAGATAATAA ATCGAAGGGG TGAGCAGCCC
    CCCACTGAGC CACTGGAGGA GTGGAAACCA GCCGGTAGTC AAATTGACCC GTGCCCAGGT
    AATGGACTCT GTTCTGTTTG TGTTACAGAT GTAAAAGGAC CACCTACGCA CAGGCTGTCC
    TGCGGCCAGT CCCCCTACAC CGAGACGACA ACATGGGAGA GGAAGTACTG CATCCTGACT
    GACAGCCAGC TGGTGCTGCT CAACAGGGAG AAGGAGGTGC CCACCGAGGG GTTGCAGGAG
    CCGCAGGATG CAACCAAAGG CCGCTGTCTG CGCCGCACGG TGAGCGTGCC CTCCGAGGGC
    CAGTTCCCCG AGTACCCACC AGATGGGGCC GCCAAGCTGG AGGTCCCGGC AGAGAGATCT
    CCTCGCAGAC GGAGCATCTC TGGGACCAGC ACATCTGAGA AAACCAACTC CATGGATGCT
    GCAAATACCT CTCCTTTCAA AGTATCGGGC TTCTTCAGTA AACGTCTGAA AGGGTCCATC
    AAGAGGACAA AGAGTCAGTC AAAGCTGGAC AGGAACACAA GTTTCCGCCT CCCATCCCTC
    CGCAATGCTG ATGACAGGTC ACGTGGGCTG CCGAAGCTGA AGGAGTCCCG CTCTCATGAG
    TCCCTGCTGA GCCCCGGCAG CGCAGTGGAA GCCCTGGACC TTGGGGCAGA AGAAAAGGTC
    TTTGTAAAGC CACTGCACAG CAGCATCCTC GGACAGGACT TCTGCTTTGA GGTAACCTAC
    TCCAGCGGCA GTAAATGCTT CAGCTGTTCC TCTGCTGCAG AGAGGGATAA GTGGATGGAA
    AACCTACGCA GAACAGTGCA GCCGAATAAG GACAACTGCC GTCGAGCTGA AAATGTGCTC
    CGTCTCTGGA TCATTGAGGC AAAGGACCTG GCCCCCAAGA AGAAATACTT CTGTGAGCTG
    TGCCTTGATG ACACTCTCTT TGCCCGCACC ACCAGCAAAA CGAAAGCAGA TAACATTTTC
    TGGGGGGAGC ACTTTGAGTT CTACGGCCTC CCAGCTCTTC ACAGCATCAC GGTTCACATC
    TACAAGGATG TGGAGAAGAA AAAGAAAAAG GACAAAAACA ATTACGTCGG CCTGGTCAAC
    ATTCCCATGG CCACTGTAAC GGGGCGCCAG TTTGTGGAAA AATGGTACCC AGTCAGCACA
    CCTACACCCA ACAAAGGGAA ATCAGGAGGC CCTTCCATTC GCATCAAGTC GCGTTATCAG
    ACCATCACCA TCTTACCCAT GGAGCAATAC AAGGAGTTTG CAGAGTTTGT TACCAGCAAC
    TACACGATGC TGTGCTCTGT GCTGGAGCCA GTCATCAGCG TAAGGAATAA GGAAGAGATG
    GCTTGTGCAA TGGTGCACAT TCTTCAGAGC ACGGGGAGAG CCAAGGACTT CCTAACGGAT
    CTGGTGATGT CTGAGGTGGA TCGCTGCGGG GAACACGATG TCTTGATCTT CAGGGAGAAC
    ACACTTGCTA CCAAAGCTAT TGAGGAATAC CTGAAGTTGG TAGGGCAGAA GTATCTCCAT
    GATGCGCTAG GGGAGTTTAT CAAGGCTTTG TATGAGTCAG ATGAAAACTG TGAAGTGGAC
    CCCAGCAAAT GTTCATCCAG TGAATTAACA GACCATCAGA GCAACCTGAA AATGTGTTGT
    GAGCTGGCCT TCTGCAAGAT TATCAATTCC TACTGCGTGT TCCCTCGTGA GCTGAAGGAG
    GTGTTTGCAT CTTGGAAGCA GCAGTGCCTG AGCAGGGGCA AGCAGGACAT CAGCGAGCGG
    CTGATCAGTG CCTCCCTGTT CCTCCGCTTC CTGTGCCCCG CCATCATGTC CCCCAGCCTC
    TTCAGCCTCA TGCAGGAGTA TCCTGACGAC CGGACATCGC GCACGCTCAC ACTCATTGCT
    AAAGTCATTC AGAATCTCGC CAATTTTGCT AAGTTTGGTA ACAAGGAAGA GTACATGGCT
    TTCATGAACG ACTTTCTGGA ACATGAGTGG GGGGGAATGA AGCGATTTCT CCTGGAGATC
    TCTAATCCAG ATACCATCTC AAACATGCCT GGATTTGAGG GCTATATCGA CCTGGGCAGA
    GAGCTCTCAG TTTTGCATTC CCTCTTATGG GAGGTTGTGT CCCAACTTGA TAAGGCGACC
    GTGGCAAAAC TGGGACCTCT TCCTCGTATT CTTGCCGACA TCACCAAATC AATGACCAAC
    CCTACGCCAA TACAGCAGCA GCTGAGGCGT TTCACTGAGC ACAGCTCTAG TCCAAACGTC
    AGTGGCAGTC TCTCCTCAGG GCTTCAGAAG ATATTCGAGG ACCCCACTGA TGGTGACTTG
    CATAAACTGA AGTCTCCAAC CCAGGAAAAC ATAGATGGCT ATTTCAGGGG CAAGACCTTG
    CTGTTGGTTC AGCAGGCGTC CACCCAGAGC ATGACTTACT CAGATAAGGA CGAAAGAGAG
    AGCATCTTGC CCAACGGACG TAGCATCTCC CTCATGGACC TGCAGGATCC CCATACCGCT
    CACAGCGACC ACGCTTCCAT GATGCACGAC GTGCCTTTGC GCTTGGCCGG CAGCCAGCTC
    TCCATCACCC AGGTGGCCAA CATCAAGCAG CTGTGGGAGA CCCAGAGCAC GCCCCAAAGC
    GCTCCCCAGG TGAGGAGGCC GCTGCACCCG GCTTTGAACC AGCAGGGCAG CCTCCAGCCT
    CTGTCATTCC AGAACCCGGT GTACCACCTC AACAACCCAC CTGCGCCGTT GCCCAAGGCC
    TCCGTGGACT CCAGCCTGGA GAACCTGAGC ACCGCCAGCT CCCAGAGCCG AAGCAACAGC
    GAAGACTTCA AGCTCAGCGG GCCCAGCAAC AGCAGCATGG AGGACTTCAC CAAGCGCAGC
    ACGCAGAGCG AGGACTTCTC TAGGCGGCAC ACGGTCCCGG ACAAGCACGT CCCCATCGCG
    TTGCCCCGCC AGAACAGCAG CAGCCAGGCA CAGATCCGCA AAATGGACCA GGCCGGGTTA
    GGGGCCAGGG CCAAAGCGCC GCAGTCGCTG CCGCACAGCG CGTCCCTGCG CAGCACGGGC
    AGCATGTCGG CCGTGTCGGG GGCCGTGGTG ACTGAGCCCG TCCAGAACGG CAGCCGCTCC
    CGGCAGCAGT CGTCCTCCTC CAGGGAGAGC CCTGTGCCGA AGGTGAGGGC CATTCAGAGG
    CAGCAAACGC AGCAGGTCCA GTCTCCTGTG GACTCAGCCA CAATGTCACC AGTGGAAAGG
    ACAGCAGCGT GGGTTCTCAA TAACGGGCAG TACGAGGAGG TCGAGGAGGA CACAGAGCAG
    AACCCAGATG AAGCCAAGCA TGCTGAGAAG TACGAACAAG AGATAGCAAA GCTGAAGGAA
    CGCTTGAAAG TGTCGAGCCG CCGGCTGGAG GAGTACGAGC GGCGGCTCCT GGTGCAGGAG
    CAGCAAATGC AGAAGCTGCT GATGGAGTAC AAGTCCAGGC TGGAGGACAG CGAGGAGAGA
    CTGCGCAGGC AACAGGAGGA GAAGGACAGC CAGATGAAAA GCATTATCAG CAGGCTCATG
    GCAGTTGAAG AGGAACTGAA GAAAGATCAT GCAGAGATGC AAGCTGTCAT CGATGCAAAG
    CAGAAAATTA TTGATGCACA GGAGAAGCGG ATCGTGTCCC TGGACTCTGC CAACACGCGG
    CTGATGAGTG CCCTGACGCA GGTGAAGGAG CGCTACAGCA TGCAAGTCCG CAATGGCATC
    TCCCCCACCA ACCCCACCAA GCTTTCCATC ACGGAGAATG GTGAATTCAA AAACAGCAGC
    TGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa152365
    Clone ID Related Accession (Same CDS sequence) XM_015290512.2
    Accession Version XM_015290512.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4266bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product ras GTPase-activating protein nGAP isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006095.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015290512.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAGTGCTT TTAAAAAGTA TTACAGTCTC TCTCCCAGGA CCTTTAGGAG GTCTTTGTCG 
    GAGCAAGATG CTGGCACCGC TGCCTTAAAG GACTATAATT GGTACCACCA AATGAGAAGC
    AAGAAGGGGA GCAAACAAAC AGCTTTGGAC CGAGAGAAAG CACTCGGAGC GTACAGGTGG
    CAAACACATC CCCGTCTTCC CTGGAGGACA AGCAGAAGAG CACACTCTTG CAGCCTCAGA
    GGGGAAGGTA ATGATTCGAG TCTTGTCAAA AGTTCTGCAA GCATGGATGC TACCAGCGCC
    GACGATCTCC ACCAAGACTC AACGGAGATG CTCTTCCAGG CAAGGACCAG ATCACCGCGG
    GTTCCCTTCA ACCGCTCCCT GTCTGAACCT GTGCCGCACT CTGCAAACAG GAATGCCAAG
    CCCTTTCTGC AGGCGGTGCG CTCTGTGGAT TGTGAGCAGC ACAGCTATTT TGTCCGTGGC
    ATCTTCTCAA CTCTCTCAAG TGGCTTTTCA AAGATAATAA ATCGAAGGGG TGAGCAGCCC
    CCCACTGAGC CACTGGAGGA GTGGAAACCA GCCGGTAGTC AAATTGACCC GTGCCCAGAT
    GTAAAAGGAC CACCTACGCA CAGGCTGTCC TGCGGCCAGT CCCCCTACAC CGAGACGACA
    ACATGGGAGA GGAAGTACTG CATCCTGACT GACAGCCAGC TGGTGCTGCT CAACAGGGAG
    AAGGAGGTGC CCACCGAGGG GTTGCAGGAG CCGCAGGATG CAACCAAAGG CCGCTGTCTG
    CGCCGCACGG TGAGCGTGCC CTCCGAGGGC CAGTTCCCCG AGTACCCACC AGATGGGGCC
    GCCAAGCTGG AGGTCCCGGC AGAGAGATCT CCTCGCAGAC GGAGCATCTC TGGGACCAGC
    ACATCTGAGA AAACCAACTC CATGGATGCT GCAAATACCT CTCCTTTCAA AGTATCGGGC
    TTCTTCAGTA AACGTCTGAA AGGGTCCATC AAGAGGACAA AGAGTCAGTC AAAGCTGGAC
    AGGAACACAA GTTTCCGCCT CCCATCCCTC CGCAATGCTG ATGACAGGTC ACGTGGGCTG
    CCGAAGCTGA AGGAGTCCCG CTCTCATGAG TCCCTGCTGA GCCCCGGCAG CGCAGTGGAA
    GCCCTGGACC TTGGGGCAGA AGAAAAGGTC TTTGTAAAGC CACTGCACAG CAGCATCCTC
    GGACAGGACT TCTGCTTTGA GGTAACCTAC TCCAGCGGCA GTAAATGCTT CAGCTGTTCC
    TCTGCTGCAG AGAGGGATAA GTGGATGGAA AACCTACGCA GAACAGTGCA GCCGAATAAG
    GACAACTGCC GTCGAGCTGA AAATGTGCTC CGTCTCTGGA TCATTGAGGC AAAGGACCTG
    GCCCCCAAGA AGAAATACTT CTGTGAGCTG TGCCTTGATG ACACTCTCTT TGCCCGCACC
    ACCAGCAAAA CGAAAGCAGA TAACATTTTC TGGGGGGAGC ACTTTGAGTT CTACGGCCTC
    CCAGCTCTTC ACAGCATCAC GGTTCACATC TACAAGGATG TGGAGAAGAA AAAGAAAAAG
    GACAAAAACA ATTACGTCGG CCTGGTCAAC ATTCCCATGG CCACTGTAAC GGGGCGCCAG
    TTTGTGGAAA AATGGTACCC AGTCAGCACA CCTACACCCA ACAAAGGGAA ATCAGGAGGC
    CCTTCCATTC GCATCAAGTC GCGTTATCAG ACCATCACCA TCTTACCCAT GGAGCAATAC
    AAGGAGTTTG CAGAGTTTGT TACCAGCAAC TACACGATGC TGTGCTCTGT GCTGGAGCCA
    GTCATCAGCG TAAGGAATAA GGAAGAGATG GCTTGTGCAA TGGTGCACAT TCTTCAGAGC
    ACGGGGAGAG CCAAGGACTT CCTAACGGAT CTGGTGATGT CTGAGGTGGA TCGCTGCGGG
    GAACACGATG TCTTGATCTT CAGGGAGAAC ACACTTGCTA CCAAAGCTAT TGAGGAATAC
    CTGAAGTTGG TAGGGCAGAA GTATCTCCAT GATGCGCTAG GGGAGTTTAT CAAGGCTTTG
    TATGAGTCAG ATGAAAACTG TGAAGTGGAC CCCAGCAAAT GTTCATCCAG TGAATTAACA
    GACCATCAGA GCAACCTGAA AATGTGTTGT GAGCTGGCCT TCTGCAAGAT TATCAATTCC
    TACTGCGTGT TCCCTCGTGA GCTGAAGGAG GTGTTTGCAT CTTGGAAGCA GCAGTGCCTG
    AGCAGGGGCA AGCAGGACAT CAGCGAGCGG CTGATCAGTG CCTCCCTGTT CCTCCGCTTC
    CTGTGCCCCG CCATCATGTC CCCCAGCCTC TTCAGCCTCA TGCAGGAGTA TCCTGACGAC
    CGGACATCGC GCACGCTCAC ACTCATTGCT AAAGTCATTC AGAATCTCGC CAATTTTGCT
    AAGTTTGGTA ACAAGGAAGA GTACATGGCT TTCATGAACG ACTTTCTGGA ACATGAGTGG
    GGGGGAATGA AGCGATTTCT CCTGGAGATC TCTAATCCAG ATACCATCTC AAACATGCCT
    GGATTTGAGG GCTATATCGA CCTGGGCAGA GAGCTCTCAG TTTTGCATTC CCTCTTATGG
    GAGGTTGTGT CCCAACTTGA TAAGGGTGAA AATTCCTTCC TACAGGCGAC CGTGGCAAAA
    CTGGGACCTC TTCCTCGTAT TCTTGCCGAC ATCACCAAAT CAATGACCAA CCCTACGCCA
    ATACAGCAGC AGCTGAGGCG TTTCACTGAG CACAGCTCTA GTCCAAACGT CAGTGGCAGT
    CTCTCCTCAG GGCTTCAGAA GATATTCGAG GACCCCACTG ATGGTGACTT GCATAAACTG
    AAGTCTCCAA CCCAGGAAAA CATAGATGGC TATTTCAGGG GCAAGACCTT GCTGTTGGTT
    CAGCAGGCGT CCACCCAGAG CATGACTTAC TCAGATAAGG ACGAAAGAGA GAGCATCTTG
    CCCAACGGAC GTAGCATCTC CCTCATGGAC CTGCAGGATC CCCATACCGC TCACAGCGAC
    CACGCTTCCA TGATGCACGA CGTGCCTTTG CGCTTGGCCG GCAGCCAGCT CTCCATCACC
    CAGGTGGCCA ACATCAAGCA GCTGTGGGAG ACCCAGAGCA CGCCCCAAAG CGCTCCCCAG
    GTGAGGAGGC CGCTGCACCC GGCTTTGAAC CAGCAGGGCA GCCTCCAGCC TCTGTCATTC
    CAGAACCCGG TGTACCACCT CAACAACCCA CCTGCGCCGT TGCCCAAGGC CTCCGTGGAC
    TCCAGCCTGG AGAACCTGAG CACCGCCAGC TCCCAGAGCC GAAGCAACAG CGAAGACTTC
    AAGCTCAGCG GGCCCAGCAA CAGCAGCATG GAGGACTTCA CCAAGCGCAG CACGCAGAGC
    GAGGACTTCT CTAGGCGGCA CACGGTCCCG GACAAGCACG TCCCCATCGC GTTGCCCCGC
    CAGAACAGCA GCAGCCAGGC ACAGATCCGC AAAATGGACC AGGCCGGGTT AGGGGCCAGG
    GCCAAAGCGC CGCAGTCGCT GCCGCACAGC GCGTCCCTGC GCAGCACGGG CAGCATGTCG
    GCCGTGTCGG GGGCCGTGGT GACTGAGCCC GTCCAGAACG GCAGCCGCTC CCGGCAGCAG
    TCGTCCTCCT CCAGGGAGAG CCCTGTGCCG AAGGTGAGGG CCATTCAGAG GCAGCAAACG
    CAGCAGGTCC AGTCTCCTGT GGACTCAGCC ACAATGTCAC CAGTGGAAAG GACAGCAGCG
    TGGGTTCTCA ATAACGGGCA GTACGAGGAG GTCGAGGAGG ACACAGAGCA GAACCCAGAT
    GAAGCCAAGC ATGCTGAGAA GTACGAACAA GAGATAGCAA AGCTGAAGGA ACGCTTGAAA
    GTGTCGAGCC GCCGGCTGGA GGAGTACGAG CGGCGGCTCC TGGTGCAGGA GCAGCAAATG
    CAGAAGCTGC TGATGGAGTA CAAGTCCAGG CTGGAGGACA GCGAGGAGAG ACTGCGCAGG
    CAACAGGAGG AGAAGGACAG CCAGATGAAA AGCATTATCA GCAGGCTCAT GGCAGTTGAA
    GAGGAACTGA AGAAAGATCA TGCAGAGATG CAAGCTGTCA TCGATGCAAA GCAGAAAATT
    ATTGATGCAC AGGTCATTAA TGGGAATGAG ATAATTCAAA CAGGAGAAGC GGATCGTGTC
    CCTGGACTCT GCCAACACGC GGCTGATGAG TGCCCTGACG CAGGTGAAGG AGCGCTACAG
    CATGCAAGTC CGCAATGGCA TCTCCCCCAC CAACCCCACC AAGCTTTCCA TCACGGAGAA
    TGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015145998.1
    CDS635..4900
    Translation

    Target ORF information:

    RefSeq Version XM_015290512.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015290512.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAGTGCTT TTAAAAAGTA TTACAGTCTC TCTCCCAGGA CCTTTAGGAG GTCTTTGTCG 
    GAGCAAGATG CTGGCACCGC TGCCTTAAAG GACTATAATT GGTACCACCA AATGAGAAGC
    AAGAAGGGGA GCAAACAAAC AGCTTTGGAC CGAGAGAAAG CACTCGGAGC GTACAGGTGG
    CAAACACATC CCCGTCTTCC CTGGAGGACA AGCAGAAGAG CACACTCTTG CAGCCTCAGA
    GGGGAAGGTA ATGATTCGAG TCTTGTCAAA AGTTCTGCAA GCATGGATGC TACCAGCGCC
    GACGATCTCC ACCAAGACTC AACGGAGATG CTCTTCCAGG CAAGGACCAG ATCACCGCGG
    GTTCCCTTCA ACCGCTCCCT GTCTGAACCT GTGCCGCACT CTGCAAACAG GAATGCCAAG
    CCCTTTCTGC AGGCGGTGCG CTCTGTGGAT TGTGAGCAGC ACAGCTATTT TGTCCGTGGC
    ATCTTCTCAA CTCTCTCAAG TGGCTTTTCA AAGATAATAA ATCGAAGGGG TGAGCAGCCC
    CCCACTGAGC CACTGGAGGA GTGGAAACCA GCCGGTAGTC AAATTGACCC GTGCCCAGAT
    GTAAAAGGAC CACCTACGCA CAGGCTGTCC TGCGGCCAGT CCCCCTACAC CGAGACGACA
    ACATGGGAGA GGAAGTACTG CATCCTGACT GACAGCCAGC TGGTGCTGCT CAACAGGGAG
    AAGGAGGTGC CCACCGAGGG GTTGCAGGAG CCGCAGGATG CAACCAAAGG CCGCTGTCTG
    CGCCGCACGG TGAGCGTGCC CTCCGAGGGC CAGTTCCCCG AGTACCCACC AGATGGGGCC
    GCCAAGCTGG AGGTCCCGGC AGAGAGATCT CCTCGCAGAC GGAGCATCTC TGGGACCAGC
    ACATCTGAGA AAACCAACTC CATGGATGCT GCAAATACCT CTCCTTTCAA AGTATCGGGC
    TTCTTCAGTA AACGTCTGAA AGGGTCCATC AAGAGGACAA AGAGTCAGTC AAAGCTGGAC
    AGGAACACAA GTTTCCGCCT CCCATCCCTC CGCAATGCTG ATGACAGGTC ACGTGGGCTG
    CCGAAGCTGA AGGAGTCCCG CTCTCATGAG TCCCTGCTGA GCCCCGGCAG CGCAGTGGAA
    GCCCTGGACC TTGGGGCAGA AGAAAAGGTC TTTGTAAAGC CACTGCACAG CAGCATCCTC
    GGACAGGACT TCTGCTTTGA GGTAACCTAC TCCAGCGGCA GTAAATGCTT CAGCTGTTCC
    TCTGCTGCAG AGAGGGATAA GTGGATGGAA AACCTACGCA GAACAGTGCA GCCGAATAAG
    GACAACTGCC GTCGAGCTGA AAATGTGCTC CGTCTCTGGA TCATTGAGGC AAAGGACCTG
    GCCCCCAAGA AGAAATACTT CTGTGAGCTG TGCCTTGATG ACACTCTCTT TGCCCGCACC
    ACCAGCAAAA CGAAAGCAGA TAACATTTTC TGGGGGGAGC ACTTTGAGTT CTACGGCCTC
    CCAGCTCTTC ACAGCATCAC GGTTCACATC TACAAGGATG TGGAGAAGAA AAAGAAAAAG
    GACAAAAACA ATTACGTCGG CCTGGTCAAC ATTCCCATGG CCACTGTAAC GGGGCGCCAG
    TTTGTGGAAA AATGGTACCC AGTCAGCACA CCTACACCCA ACAAAGGGAA ATCAGGAGGC
    CCTTCCATTC GCATCAAGTC GCGTTATCAG ACCATCACCA TCTTACCCAT GGAGCAATAC
    AAGGAGTTTG CAGAGTTTGT TACCAGCAAC TACACGATGC TGTGCTCTGT GCTGGAGCCA
    GTCATCAGCG TAAGGAATAA GGAAGAGATG GCTTGTGCAA TGGTGCACAT TCTTCAGAGC
    ACGGGGAGAG CCAAGGACTT CCTAACGGAT CTGGTGATGT CTGAGGTGGA TCGCTGCGGG
    GAACACGATG TCTTGATCTT CAGGGAGAAC ACACTTGCTA CCAAAGCTAT TGAGGAATAC
    CTGAAGTTGG TAGGGCAGAA GTATCTCCAT GATGCGCTAG GGGAGTTTAT CAAGGCTTTG
    TATGAGTCAG ATGAAAACTG TGAAGTGGAC CCCAGCAAAT GTTCATCCAG TGAATTAACA
    GACCATCAGA GCAACCTGAA AATGTGTTGT GAGCTGGCCT TCTGCAAGAT TATCAATTCC
    TACTGCGTGT TCCCTCGTGA GCTGAAGGAG GTGTTTGCAT CTTGGAAGCA GCAGTGCCTG
    AGCAGGGGCA AGCAGGACAT CAGCGAGCGG CTGATCAGTG CCTCCCTGTT CCTCCGCTTC
    CTGTGCCCCG CCATCATGTC CCCCAGCCTC TTCAGCCTCA TGCAGGAGTA TCCTGACGAC
    CGGACATCGC GCACGCTCAC ACTCATTGCT AAAGTCATTC AGAATCTCGC CAATTTTGCT
    AAGTTTGGTA ACAAGGAAGA GTACATGGCT TTCATGAACG ACTTTCTGGA ACATGAGTGG
    GGGGGAATGA AGCGATTTCT CCTGGAGATC TCTAATCCAG ATACCATCTC AAACATGCCT
    GGATTTGAGG GCTATATCGA CCTGGGCAGA GAGCTCTCAG TTTTGCATTC CCTCTTATGG
    GAGGTTGTGT CCCAACTTGA TAAGGGTGAA AATTCCTTCC TACAGGCGAC CGTGGCAAAA
    CTGGGACCTC TTCCTCGTAT TCTTGCCGAC ATCACCAAAT CAATGACCAA CCCTACGCCA
    ATACAGCAGC AGCTGAGGCG TTTCACTGAG CACAGCTCTA GTCCAAACGT CAGTGGCAGT
    CTCTCCTCAG GGCTTCAGAA GATATTCGAG GACCCCACTG ATGGTGACTT GCATAAACTG
    AAGTCTCCAA CCCAGGAAAA CATAGATGGC TATTTCAGGG GCAAGACCTT GCTGTTGGTT
    CAGCAGGCGT CCACCCAGAG CATGACTTAC TCAGATAAGG ACGAAAGAGA GAGCATCTTG
    CCCAACGGAC GTAGCATCTC CCTCATGGAC CTGCAGGATC CCCATACCGC TCACAGCGAC
    CACGCTTCCA TGATGCACGA CGTGCCTTTG CGCTTGGCCG GCAGCCAGCT CTCCATCACC
    CAGGTGGCCA ACATCAAGCA GCTGTGGGAG ACCCAGAGCA CGCCCCAAAG CGCTCCCCAG
    GTGAGGAGGC CGCTGCACCC GGCTTTGAAC CAGCAGGGCA GCCTCCAGCC TCTGTCATTC
    CAGAACCCGG TGTACCACCT CAACAACCCA CCTGCGCCGT TGCCCAAGGC CTCCGTGGAC
    TCCAGCCTGG AGAACCTGAG CACCGCCAGC TCCCAGAGCC GAAGCAACAG CGAAGACTTC
    AAGCTCAGCG GGCCCAGCAA CAGCAGCATG GAGGACTTCA CCAAGCGCAG CACGCAGAGC
    GAGGACTTCT CTAGGCGGCA CACGGTCCCG GACAAGCACG TCCCCATCGC GTTGCCCCGC
    CAGAACAGCA GCAGCCAGGC ACAGATCCGC AAAATGGACC AGGCCGGGTT AGGGGCCAGG
    GCCAAAGCGC CGCAGTCGCT GCCGCACAGC GCGTCCCTGC GCAGCACGGG CAGCATGTCG
    GCCGTGTCGG GGGCCGTGGT GACTGAGCCC GTCCAGAACG GCAGCCGCTC CCGGCAGCAG
    TCGTCCTCCT CCAGGGAGAG CCCTGTGCCG AAGGTGAGGG CCATTCAGAG GCAGCAAACG
    CAGCAGGTCC AGTCTCCTGT GGACTCAGCC ACAATGTCAC CAGTGGAAAG GACAGCAGCG
    TGGGTTCTCA ATAACGGGCA GTACGAGGAG GTCGAGGAGG ACACAGAGCA GAACCCAGAT
    GAAGCCAAGC ATGCTGAGAA GTACGAACAA GAGATAGCAA AGCTGAAGGA ACGCTTGAAA
    GTGTCGAGCC GCCGGCTGGA GGAGTACGAG CGGCGGCTCC TGGTGCAGGA GCAGCAAATG
    CAGAAGCTGC TGATGGAGTA CAAGTCCAGG CTGGAGGACA GCGAGGAGAG ACTGCGCAGG
    CAACAGGAGG AGAAGGACAG CCAGATGAAA AGCATTATCA GCAGGCTCAT GGCAGTTGAA
    GAGGAACTGA AGAAAGATCA TGCAGAGATG CAAGCTGTCA TCGATGCAAA GCAGAAAATT
    ATTGATGCAC AGGTCATTAA TGGGAATGAG ATAATTCAAA CAGGAGAAGC GGATCGTGTC
    CCTGGACTCT GCCAACACGC GGCTGATGAG TGCCCTGACG CAGGTGAAGG AGCGCTACAG
    CATGCAAGTC CGCAATGGCA TCTCCCCCAC CAACCCCACC AAGCTTTCCA TCACGGAGAA
    TGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa152366
    Clone ID Related Accession (Same CDS sequence) XM_015290513.2
    Accession Version XM_015290513.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3828bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product ras GTPase-activating protein nGAP isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006095.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015290513.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGGCGG CCCCCGCGGC GAGCGAGCCG GCGGCCTGGG CGGCCGAGGC GCTGGCGGAG 
    CCGGAGCTGG GCTCGGACGA GCTGGAGGCG GCGGGCGGAG CGCTGGACCG CGTCCTGCTG
    GACTCGGTGT GCCAGCAGCA GGGCTGGGTC CGCGTCTACG ATGTAAAAGG ACCACCTACG
    CACAGGCTGT CCTGCGGCCA GTCCCCCTAC ACCGAGACGA CAACATGGGA GAGGAAGTAC
    TGCATCCTGA CTGACAGCCA GCTGGTGCTG CTCAACAGGG AGAAGGAGGT GCCCACCGAG
    GGGTTGCAGG AGCCGCAGGA TGCAACCAAA GGCCGCTGTC TGCGCCGCAC GGTGAGCGTG
    CCCTCCGAGG GCCAGTTCCC CGAGTACCCA CCAGATGGGG CCGCCAAGCT GGAGGTCCCG
    GCAGAGAGAT CTCCTCGCAG ACGGAGCATC TCTGGGACCA GCACATCTGA GAAAACCAAC
    TCCATGGATG CTGCAAATAC CTCTCCTTTC AAAGTATCGG GCTTCTTCAG TAAACGTCTG
    AAAGGGTCCA TCAAGAGGAC AAAGAGTCAG TCAAAGCTGG ACAGGAACAC AAGTTTCCGC
    CTCCCATCCC TCCGCAATGC TGATGACAGG TCACGTGGGC TGCCGAAGCT GAAGGAGTCC
    CGCTCTCATG AGTCCCTGCT GAGCCCCGGC AGCGCAGTGG AAGCCCTGGA CCTTGGGGCA
    GAAGAAAAGG TCTTTGTAAA GCCACTGCAC AGCAGCATCC TCGGACAGGA CTTCTGCTTT
    GAGGTAACCT ACTCCAGCGG CAGTAAATGC TTCAGCTGTT CCTCTGCTGC AGAGAGGGAT
    AAGTGGATGG AAAACCTACG CAGAACAGTG CAGCCGAATA AGGACAACTG CCGTCGAGCT
    GAAAATGTGC TCCGTCTCTG GATCATTGAG GCAAAGGACC TGGCCCCCAA GAAGAAATAC
    TTCTGTGAGC TGTGCCTTGA TGACACTCTC TTTGCCCGCA CCACCAGCAA AACGAAAGCA
    GATAACATTT TCTGGGGGGA GCACTTTGAG TTCTACGGCC TCCCAGCTCT TCACAGCATC
    ACGGTTCACA TCTACAAGGA TGTGGAGAAG AAAAAGAAAA AGGACAAAAA CAATTACGTC
    GGCCTGGTCA ACATTCCCAT GGCCACTGTA ACGGGGCGCC AGTTTGTGGA AAAATGGTAC
    CCAGTCAGCA CACCTACACC CAACAAAGGG AAATCAGGAG GCCCTTCCAT TCGCATCAAG
    TCGCGTTATC AGACCATCAC CATCTTACCC ATGGAGCAAT ACAAGGAGTT TGCAGAGTTT
    GTTACCAGCA ACTACACGAT GCTGTGCTCT GTGCTGGAGC CAGTCATCAG CGTAAGGAAT
    AAGGAAGAGA TGGCTTGTGC AATGGTGCAC ATTCTTCAGA GCACGGGGAG AGCCAAGGAC
    TTCCTAACGG ATCTGGTGAT GTCTGAGGTG GATCGCTGCG GGGAACACGA TGTCTTGATC
    TTCAGGGAGA ACACACTTGC TACCAAAGCT ATTGAGGAAT ACCTGAAGTT GGTAGGGCAG
    AAGTATCTCC ATGATGCGCT AGGGGAGTTT ATCAAGGCTT TGTATGAGTC AGATGAAAAC
    TGTGAAGTGG ACCCCAGCAA ATGTTCATCC AGTGAATTAA CAGACCATCA GAGCAACCTG
    AAAATGTGTT GTGAGCTGGC CTTCTGCAAG ATTATCAATT CCTACTGCGT GTTCCCTCGT
    GAGCTGAAGG AGGTGTTTGC ATCTTGGAAG CAGCAGTGCC TGAGCAGGGG CAAGCAGGAC
    ATCAGCGAGC GGCTGATCAG TGCCTCCCTG TTCCTCCGCT TCCTGTGCCC CGCCATCATG
    TCCCCCAGCC TCTTCAGCCT CATGCAGGAG TATCCTGACG ACCGGACATC GCGCACGCTC
    ACACTCATTG CTAAAGTCAT TCAGAATCTC GCCAATTTTG CTAAGTTTGG TAACAAGGAA
    GAGTACATGG CTTTCATGAA CGACTTTCTG GAACATGAGT GGGGGGGAAT GAAGCGATTT
    CTCCTGGAGA TCTCTAATCC AGATACCATC TCAAACATGC CTGGATTTGA GGGCTATATC
    GACCTGGGCA GAGAGCTCTC AGTTTTGCAT TCCCTCTTAT GGGAGGTTGT GTCCCAACTT
    GATAAGGGTG AAAATTCCTT CCTACAGGCG ACCGTGGCAA AACTGGGACC TCTTCCTCGT
    ATTCTTGCCG ACATCACCAA ATCAATGACC AACCCTACGC CAATACAGCA GCAGCTGAGG
    CGTTTCACTG AGCACAGCTC TAGTCCAAAC GTCAGTGGCA GTCTCTCCTC AGGGCTTCAG
    AAGATATTCG AGGACCCCAC TGATGGTGAC TTGCATAAAC TGAAGTCTCC AACCCAGGAA
    AACATAGATG GCTATTTCAG GGGCAAGACC TTGCTGTTGG TTCAGCAGGC GTCCACCCAG
    AGCATGACTT ACTCAGATAA GGACGAAAGA GAGAGCATCT TGCCCAACGG ACGTAGCATC
    TCCCTCATGG ACCTGCAGGA TCCCCATACC GCTCACAGCG ACCACGCTTC CATGATGCAC
    GACGTGCCTT TGCGCTTGGC CGGCAGCCAG CTCTCCATCA CCCAGGTGGC CAACATCAAG
    CAGCTGTGGG AGACCCAGAG CACGCCCCAA AGCGCTCCCC AGGTGAGGAG GCCGCTGCAC
    CCGGCTTTGA ACCAGCAGGG CAGCCTCCAG CCTCTGTCAT TCCAGAACCC GGTGTACCAC
    CTCAACAACC CACCTGCGCC GTTGCCCAAG GCCTCCGTGG ACTCCAGCCT GGAGAACCTG
    AGCACCGCCA GCTCCCAGAG CCGAAGCAAC AGCGAAGACT TCAAGCTCAG CGGGCCCAGC
    AACAGCAGCA TGGAGGACTT CACCAAGCGC AGCACGCAGA GCGAGGACTT CTCTAGGCGG
    CACACGGTCC CGGACAAGCA CGTCCCCATC GCGTTGCCCC GCCAGAACAG CAGCAGCCAG
    GCACAGATCC GCAAAATGGA CCAGGCCGGG TTAGGGGCCA GGGCCAAAGC GCCGCAGTCG
    CTGCCGCACA GCGCGTCCCT GCGCAGCACG GGCAGCATGT CGGCCGTGTC GGGGGCCGTG
    GTGACTGAGC CCGTCCAGAA CGGCAGCCGC TCCCGGCAGC AGTCGTCCTC CTCCAGGGAG
    AGCCCTGTGC CGAAGGTGAG GGCCATTCAG AGGCAGCAAA CGCAGCAGGT CCAGTCTCCT
    GTGGACTCAG CCACAATGTC ACCAGTGGAA AGGACAGCAG CGTGGGTTCT CAATAACGGG
    CAGTACGAGG AGGTCGAGGA GGACACAGAG CAGAACCCAG ATGAAGCCAA GCATGCTGAG
    AAGTACGAAC AAGAGATAGC AAAGCTGAAG GAACGCTTGA AAGTGTCGAG CCGCCGGCTG
    GAGGAGTACG AGCGGCGGCT CCTGGTGCAG GAGCAGCAAA TGCAGAAGCT GCTGATGGAG
    TACAAGTCCA GGCTGGAGGA CAGCGAGGAG AGACTGCGCA GGCAACAGGA GGAGAAGGAC
    AGCCAGATGA AAAGCATTAT CAGCAGGCTC ATGGCAGTTG AAGAGGAACT GAAGAAAGAT
    CATGCAGAGA TGCAAGCTGT CATCGATGCA AAGCAGAAAA TTATTGATGC ACAGGTCATT
    AATGGGAATG AGATAATTCA AACAGGAGAA GCGGATCGTG TCCCTGGACT CTGCCAACAC
    GCGGCTGATG AGTGCCCTGA CGCAGGTGAA GGAGCGCTAC AGCATGCAAG TCCGCAATGG
    CATCTCCCCC ACCAACCCCA CCAAGCTTTC CATCACGGAG AATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015145999.1
    CDS75..3902
    Translation

    Target ORF information:

    RefSeq Version XM_015290513.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015290513.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGGCGG CCCCCGCGGC GAGCGAGCCG GCGGCCTGGG CGGCCGAGGC GCTGGCGGAG 
    CCGGAGCTGG GCTCGGACGA GCTGGAGGCG GCGGGCGGAG CGCTGGACCG CGTCCTGCTG
    GACTCGGTGT GCCAGCAGCA GGGCTGGGTC CGCGTCTACG ATGTAAAAGG ACCACCTACG
    CACAGGCTGT CCTGCGGCCA GTCCCCCTAC ACCGAGACGA CAACATGGGA GAGGAAGTAC
    TGCATCCTGA CTGACAGCCA GCTGGTGCTG CTCAACAGGG AGAAGGAGGT GCCCACCGAG
    GGGTTGCAGG AGCCGCAGGA TGCAACCAAA GGCCGCTGTC TGCGCCGCAC GGTGAGCGTG
    CCCTCCGAGG GCCAGTTCCC CGAGTACCCA CCAGATGGGG CCGCCAAGCT GGAGGTCCCG
    GCAGAGAGAT CTCCTCGCAG ACGGAGCATC TCTGGGACCA GCACATCTGA GAAAACCAAC
    TCCATGGATG CTGCAAATAC CTCTCCTTTC AAAGTATCGG GCTTCTTCAG TAAACGTCTG
    AAAGGGTCCA TCAAGAGGAC AAAGAGTCAG TCAAAGCTGG ACAGGAACAC AAGTTTCCGC
    CTCCCATCCC TCCGCAATGC TGATGACAGG TCACGTGGGC TGCCGAAGCT GAAGGAGTCC
    CGCTCTCATG AGTCCCTGCT GAGCCCCGGC AGCGCAGTGG AAGCCCTGGA CCTTGGGGCA
    GAAGAAAAGG TCTTTGTAAA GCCACTGCAC AGCAGCATCC TCGGACAGGA CTTCTGCTTT
    GAGGTAACCT ACTCCAGCGG CAGTAAATGC TTCAGCTGTT CCTCTGCTGC AGAGAGGGAT
    AAGTGGATGG AAAACCTACG CAGAACAGTG CAGCCGAATA AGGACAACTG CCGTCGAGCT
    GAAAATGTGC TCCGTCTCTG GATCATTGAG GCAAAGGACC TGGCCCCCAA GAAGAAATAC
    TTCTGTGAGC TGTGCCTTGA TGACACTCTC TTTGCCCGCA CCACCAGCAA AACGAAAGCA
    GATAACATTT TCTGGGGGGA GCACTTTGAG TTCTACGGCC TCCCAGCTCT TCACAGCATC
    ACGGTTCACA TCTACAAGGA TGTGGAGAAG AAAAAGAAAA AGGACAAAAA CAATTACGTC
    GGCCTGGTCA ACATTCCCAT GGCCACTGTA ACGGGGCGCC AGTTTGTGGA AAAATGGTAC
    CCAGTCAGCA CACCTACACC CAACAAAGGG AAATCAGGAG GCCCTTCCAT TCGCATCAAG
    TCGCGTTATC AGACCATCAC CATCTTACCC ATGGAGCAAT ACAAGGAGTT TGCAGAGTTT
    GTTACCAGCA ACTACACGAT GCTGTGCTCT GTGCTGGAGC CAGTCATCAG CGTAAGGAAT
    AAGGAAGAGA TGGCTTGTGC AATGGTGCAC ATTCTTCAGA GCACGGGGAG AGCCAAGGAC
    TTCCTAACGG ATCTGGTGAT GTCTGAGGTG GATCGCTGCG GGGAACACGA TGTCTTGATC
    TTCAGGGAGA ACACACTTGC TACCAAAGCT ATTGAGGAAT ACCTGAAGTT GGTAGGGCAG
    AAGTATCTCC ATGATGCGCT AGGGGAGTTT ATCAAGGCTT TGTATGAGTC AGATGAAAAC
    TGTGAAGTGG ACCCCAGCAA ATGTTCATCC AGTGAATTAA CAGACCATCA GAGCAACCTG
    AAAATGTGTT GTGAGCTGGC CTTCTGCAAG ATTATCAATT CCTACTGCGT GTTCCCTCGT
    GAGCTGAAGG AGGTGTTTGC ATCTTGGAAG CAGCAGTGCC TGAGCAGGGG CAAGCAGGAC
    ATCAGCGAGC GGCTGATCAG TGCCTCCCTG TTCCTCCGCT TCCTGTGCCC CGCCATCATG
    TCCCCCAGCC TCTTCAGCCT CATGCAGGAG TATCCTGACG ACCGGACATC GCGCACGCTC
    ACACTCATTG CTAAAGTCAT TCAGAATCTC GCCAATTTTG CTAAGTTTGG TAACAAGGAA
    GAGTACATGG CTTTCATGAA CGACTTTCTG GAACATGAGT GGGGGGGAAT GAAGCGATTT
    CTCCTGGAGA TCTCTAATCC AGATACCATC TCAAACATGC CTGGATTTGA GGGCTATATC
    GACCTGGGCA GAGAGCTCTC AGTTTTGCAT TCCCTCTTAT GGGAGGTTGT GTCCCAACTT
    GATAAGGGTG AAAATTCCTT CCTACAGGCG ACCGTGGCAA AACTGGGACC TCTTCCTCGT
    ATTCTTGCCG ACATCACCAA ATCAATGACC AACCCTACGC CAATACAGCA GCAGCTGAGG
    CGTTTCACTG AGCACAGCTC TAGTCCAAAC GTCAGTGGCA GTCTCTCCTC AGGGCTTCAG
    AAGATATTCG AGGACCCCAC TGATGGTGAC TTGCATAAAC TGAAGTCTCC AACCCAGGAA
    AACATAGATG GCTATTTCAG GGGCAAGACC TTGCTGTTGG TTCAGCAGGC GTCCACCCAG
    AGCATGACTT ACTCAGATAA GGACGAAAGA GAGAGCATCT TGCCCAACGG ACGTAGCATC
    TCCCTCATGG ACCTGCAGGA TCCCCATACC GCTCACAGCG ACCACGCTTC CATGATGCAC
    GACGTGCCTT TGCGCTTGGC CGGCAGCCAG CTCTCCATCA CCCAGGTGGC CAACATCAAG
    CAGCTGTGGG AGACCCAGAG CACGCCCCAA AGCGCTCCCC AGGTGAGGAG GCCGCTGCAC
    CCGGCTTTGA ACCAGCAGGG CAGCCTCCAG CCTCTGTCAT TCCAGAACCC GGTGTACCAC
    CTCAACAACC CACCTGCGCC GTTGCCCAAG GCCTCCGTGG ACTCCAGCCT GGAGAACCTG
    AGCACCGCCA GCTCCCAGAG CCGAAGCAAC AGCGAAGACT TCAAGCTCAG CGGGCCCAGC
    AACAGCAGCA TGGAGGACTT CACCAAGCGC AGCACGCAGA GCGAGGACTT CTCTAGGCGG
    CACACGGTCC CGGACAAGCA CGTCCCCATC GCGTTGCCCC GCCAGAACAG CAGCAGCCAG
    GCACAGATCC GCAAAATGGA CCAGGCCGGG TTAGGGGCCA GGGCCAAAGC GCCGCAGTCG
    CTGCCGCACA GCGCGTCCCT GCGCAGCACG GGCAGCATGT CGGCCGTGTC GGGGGCCGTG
    GTGACTGAGC CCGTCCAGAA CGGCAGCCGC TCCCGGCAGC AGTCGTCCTC CTCCAGGGAG
    AGCCCTGTGC CGAAGGTGAG GGCCATTCAG AGGCAGCAAA CGCAGCAGGT CCAGTCTCCT
    GTGGACTCAG CCACAATGTC ACCAGTGGAA AGGACAGCAG CGTGGGTTCT CAATAACGGG
    CAGTACGAGG AGGTCGAGGA GGACACAGAG CAGAACCCAG ATGAAGCCAA GCATGCTGAG
    AAGTACGAAC AAGAGATAGC AAAGCTGAAG GAACGCTTGA AAGTGTCGAG CCGCCGGCTG
    GAGGAGTACG AGCGGCGGCT CCTGGTGCAG GAGCAGCAAA TGCAGAAGCT GCTGATGGAG
    TACAAGTCCA GGCTGGAGGA CAGCGAGGAG AGACTGCGCA GGCAACAGGA GGAGAAGGAC
    AGCCAGATGA AAAGCATTAT CAGCAGGCTC ATGGCAGTTG AAGAGGAACT GAAGAAAGAT
    CATGCAGAGA TGCAAGCTGT CATCGATGCA AAGCAGAAAA TTATTGATGC ACAGGTCATT
    AATGGGAATG AGATAATTCA AACAGGAGAA GCGGATCGTG TCCCTGGACT CTGCCAACAC
    GCGGCTGATG AGTGCCCTGA CGCAGGTGAA GGAGCGCTAC AGCATGCAAG TCCGCAATGG
    CATCTCCCCC ACCAACCCCA CCAAGCTTTC CATCACGGAG AATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa152367
    Clone ID Related Accession (Same CDS sequence) XM_015290514.2
    Accession Version XM_015290514.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3798bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product ras GTPase-activating protein nGAP isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006095.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015290514.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGGCGG CCCCCGCGGC GAGCGAGCCG GCGGCCTGGG CGGCCGAGGC GCTGGCGGAG 
    CCGGAGCTGG GCTCGGACGA GCTGGAGGCG GCGGGCGGAG CGCTGGACCG CGTCCTGCTG
    GACTCGGTGT GCCAGCAGCA GGGCTGGGTC CGCGTCTACG ATGTAAAAGG ACCACCTACG
    CACAGGCTGT CCTGCGGCCA GTCCCCCTAC ACCGAGACGA CAACATGGGA GAGGAAGTAC
    TGCATCCTGA CTGACAGCCA GCTGGTGCTG CTCAACAGGG AGAAGGAGGT GCCCACCGAG
    GGGTTGCAGG AGCCGCAGGA TGCAACCAAA GGCCGCTGTC TGCGCCGCAC GGTGAGCGTG
    CCCTCCGAGG GCCAGTTCCC CGAGTACCCA CCAGATGGGG CCGCCAAGCT GGAGGTCCCG
    GCAGAGAGAT CTCCTCGCAG ACGGAGCATC TCTGGGACCA GCACATCTGA GAAAACCAAC
    TCCATGGATG CTGCAAATAC CTCTCCTTTC AAAGTATCGG GCTTCTTCAG TAAACGTCTG
    AAAGGGTCCA TCAAGAGGAC AAAGAGTCAG TCAAAGCTGG ACAGGAACAC AAGTTTCCGC
    CTCCCATCCC TCCGCAATGC TGATGACAGG TCACGTGGGC TGCCGAAGCT GAAGGAGTCC
    CGCTCTCATG AGTCCCTGCT GAGCCCCGGC AGCGCAGTGG AAGCCCTGGA CCTTGGGGCA
    GAAGAAAAGG TCTTTGTAAA GCCACTGCAC AGCAGCATCC TCGGACAGGA CTTCTGCTTT
    GAGGTAACCT ACTCCAGCGG CAGTAAATGC TTCAGCTGTT CCTCTGCTGC AGAGAGGGAT
    AAGTGGATGG AAAACCTACG CAGAACAGTG CAGCCGAATA AGGACAACTG CCGTCGAGCT
    GAAAATGTGC TCCGTCTCTG GATCATTGAG GCAAAGGACC TGGCCCCCAA GAAGAAATAC
    TTCTGTGAGC TGTGCCTTGA TGACACTCTC TTTGCCCGCA CCACCAGCAA AACGAAAGCA
    GATAACATTT TCTGGGGGGA GCACTTTGAG TTCTACGGCC TCCCAGCTCT TCACAGCATC
    ACGGTTCACA TCTACAAGGA TGTGGAGAAG AAAAAGAAAA AGGACAAAAA CAATTACGTC
    GGCCTGGTCA ACATTCCCAT GGCCACTGTA ACGGGGCGCC AGTTTGTGGA AAAATGGTAC
    CCAGTCAGCA CACCTACACC CAACAAAGGG AAATCAGGAG GCCCTTCCAT TCGCATCAAG
    TCGCGTTATC AGACCATCAC CATCTTACCC ATGGAGCAAT ACAAGGAGTT TGCAGAGTTT
    GTTACCAGCA ACTACACGAT GCTGTGCTCT GTGCTGGAGC CAGTCATCAG CGTAAGGAAT
    AAGGAAGAGA TGGCTTGTGC AATGGTGCAC ATTCTTCAGA GCACGGGGAG AGCCAAGGAC
    TTCCTAACGG ATCTGGTGAT GTCTGAGGTG GATCGCTGCG GGGAACACGA TGTCTTGATC
    TTCAGGGAGA ACACACTTGC TACCAAAGCT ATTGAGGAAT ACCTGAAGTT GGTAGGGCAG
    AAGTATCTCC ATGATGCGCT AGGGGAGTTT ATCAAGGCTT TGTATGAGTC AGATGAAAAC
    TGTGAAGTGG ACCCCAGCAA ATGTTCATCC AGTGAATTAA CAGACCATCA GAGCAACCTG
    AAAATGTGTT GTGAGCTGGC CTTCTGCAAG ATTATCAATT CCTACTGCGT GTTCCCTCGT
    GAGCTGAAGG AGGTGTTTGC ATCTTGGAAG CAGCAGTGCC TGAGCAGGGG CAAGCAGGAC
    ATCAGCGAGC GGCTGATCAG TGCCTCCCTG TTCCTCCGCT TCCTGTGCCC CGCCATCATG
    TCCCCCAGCC TCTTCAGCCT CATGCAGGAG TATCCTGACG ACCGGACATC GCGCACGCTC
    ACACTCATTG CTAAAGTCAT TCAGAATCTC GCCAATTTTG CTAAGTTTGG TAACAAGGAA
    GAGTACATGG CTTTCATGAA CGACTTTCTG GAACATGAGT GGGGGGGAAT GAAGCGATTT
    CTCCTGGAGA TCTCTAATCC AGATACCATC TCAAACATGC CTGGATTTGA GGGCTATATC
    GACCTGGGCA GAGAGCTCTC AGTTTTGCAT TCCCTCTTAT GGGAGGTTGT GTCCCAACTT
    GATAAGGCGA CCGTGGCAAA ACTGGGACCT CTTCCTCGTA TTCTTGCCGA CATCACCAAA
    TCAATGACCA ACCCTACGCC AATACAGCAG CAGCTGAGGC GTTTCACTGA GCACAGCTCT
    AGTCCAAACG TCAGTGGCAG TCTCTCCTCA GGGCTTCAGA AGATATTCGA GGACCCCACT
    GATGGTGACT TGCATAAACT GAAGTCTCCA ACCCAGGAAA ACATAGATGG CTATTTCAGG
    GGCAAGACCT TGCTGTTGGT TCAGCAGGCG TCCACCCAGA GCATGACTTA CTCAGATAAG
    GACGAAAGAG AGAGCATCTT GCCCAACGGA CGTAGCATCT CCCTCATGGA CCTGCAGGAT
    CCCCATACCG CTCACAGCGA CCACGCTTCC ATGATGCACG ACGTGCCTTT GCGCTTGGCC
    GGCAGCCAGC TCTCCATCAC CCAGGTGGCC AACATCAAGC AGCTGTGGGA GACCCAGAGC
    ACGCCCCAAA GCGCTCCCCA GGTGAGGAGG CCGCTGCACC CGGCTTTGAA CCAGCAGGGC
    AGCCTCCAGC CTCTGTCATT CCAGAACCCG GTGTACCACC TCAACAACCC ACCTGCGCCG
    TTGCCCAAGG CCTCCGTGGA CTCCAGCCTG GAGAACCTGA GCACCGCCAG CTCCCAGAGC
    CGAAGCAACA GCGAAGACTT CAAGCTCAGC GGGCCCAGCA ACAGCAGCAT GGAGGACTTC
    ACCAAGCGCA GCACGCAGAG CGAGGACTTC TCTAGGCGGC ACACGGTCCC GGACAAGCAC
    GTCCCCATCG CGTTGCCCCG CCAGAACAGC AGCAGCCAGG CACAGATCCG CAAAATGGAC
    CAGGCCGGGT TAGGGGCCAG GGCCAAAGCG CCGCAGTCGC TGCCGCACAG CGCGTCCCTG
    CGCAGCACGG GCAGCATGTC GGCCGTGTCG GGGGCCGTGG TGACTGAGCC CGTCCAGAAC
    GGCAGCCGCT CCCGGCAGCA GTCGTCCTCC TCCAGGGAGA GCCCTGTGCC GAAGGTGAGG
    GCCATTCAGA GGCAGCAAAC GCAGCAGGTC CAGTCTCCTG TGGACTCAGC CACAATGTCA
    CCAGTGGAAA GGACAGCAGC GTGGGTTCTC AATAACGGGC AGTACGAGGA GGTCGAGGAG
    GACACAGAGC AGAACCCAGA TGAAGCCAAG CATGCTGAGA AGTACGAACA AGAGATAGCA
    AAGCTGAAGG AACGCTTGAA AGTGTCGAGC CGCCGGCTGG AGGAGTACGA GCGGCGGCTC
    CTGGTGCAGG AGCAGCAAAT GCAGAAGCTG CTGATGGAGT ACAAGTCCAG GCTGGAGGAC
    AGCGAGGAGA GACTGCGCAG GCAACAGGAG GAGAAGGACA GCCAGATGAA AAGCATTATC
    AGCAGGCTCA TGGCAGTTGA AGAGGAACTG AAGAAAGATC ATGCAGAGAT GCAAGCTGTC
    ATCGATGCAA AGCAGAAAAT TATTGATGCA CAGGAGAAGC GGATCGTGTC CCTGGACTCT
    GCCAACACGC GGCTGATGAG TGCCCTGACG CAGGTGAAGG AGCGCTACAG CATGCAAGTC
    CGCAATGGCA TCTCCCCCAC CAACCCCACC AAGCTTTCCA TCACGGAGAA TGGTGAATTC
    AAAAACAGCA GCTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015146000.1
    CDS74..3871
    Translation

    Target ORF information:

    RefSeq Version XM_015290514.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015290514.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGGCGG CCCCCGCGGC GAGCGAGCCG GCGGCCTGGG CGGCCGAGGC GCTGGCGGAG 
    CCGGAGCTGG GCTCGGACGA GCTGGAGGCG GCGGGCGGAG CGCTGGACCG CGTCCTGCTG
    GACTCGGTGT GCCAGCAGCA GGGCTGGGTC CGCGTCTACG ATGTAAAAGG ACCACCTACG
    CACAGGCTGT CCTGCGGCCA GTCCCCCTAC ACCGAGACGA CAACATGGGA GAGGAAGTAC
    TGCATCCTGA CTGACAGCCA GCTGGTGCTG CTCAACAGGG AGAAGGAGGT GCCCACCGAG
    GGGTTGCAGG AGCCGCAGGA TGCAACCAAA GGCCGCTGTC TGCGCCGCAC GGTGAGCGTG
    CCCTCCGAGG GCCAGTTCCC CGAGTACCCA CCAGATGGGG CCGCCAAGCT GGAGGTCCCG
    GCAGAGAGAT CTCCTCGCAG ACGGAGCATC TCTGGGACCA GCACATCTGA GAAAACCAAC
    TCCATGGATG CTGCAAATAC CTCTCCTTTC AAAGTATCGG GCTTCTTCAG TAAACGTCTG
    AAAGGGTCCA TCAAGAGGAC AAAGAGTCAG TCAAAGCTGG ACAGGAACAC AAGTTTCCGC
    CTCCCATCCC TCCGCAATGC TGATGACAGG TCACGTGGGC TGCCGAAGCT GAAGGAGTCC
    CGCTCTCATG AGTCCCTGCT GAGCCCCGGC AGCGCAGTGG AAGCCCTGGA CCTTGGGGCA
    GAAGAAAAGG TCTTTGTAAA GCCACTGCAC AGCAGCATCC TCGGACAGGA CTTCTGCTTT
    GAGGTAACCT ACTCCAGCGG CAGTAAATGC TTCAGCTGTT CCTCTGCTGC AGAGAGGGAT
    AAGTGGATGG AAAACCTACG CAGAACAGTG CAGCCGAATA AGGACAACTG CCGTCGAGCT
    GAAAATGTGC TCCGTCTCTG GATCATTGAG GCAAAGGACC TGGCCCCCAA GAAGAAATAC
    TTCTGTGAGC TGTGCCTTGA TGACACTCTC TTTGCCCGCA CCACCAGCAA AACGAAAGCA
    GATAACATTT TCTGGGGGGA GCACTTTGAG TTCTACGGCC TCCCAGCTCT TCACAGCATC
    ACGGTTCACA TCTACAAGGA TGTGGAGAAG AAAAAGAAAA AGGACAAAAA CAATTACGTC
    GGCCTGGTCA ACATTCCCAT GGCCACTGTA ACGGGGCGCC AGTTTGTGGA AAAATGGTAC
    CCAGTCAGCA CACCTACACC CAACAAAGGG AAATCAGGAG GCCCTTCCAT TCGCATCAAG
    TCGCGTTATC AGACCATCAC CATCTTACCC ATGGAGCAAT ACAAGGAGTT TGCAGAGTTT
    GTTACCAGCA ACTACACGAT GCTGTGCTCT GTGCTGGAGC CAGTCATCAG CGTAAGGAAT
    AAGGAAGAGA TGGCTTGTGC AATGGTGCAC ATTCTTCAGA GCACGGGGAG AGCCAAGGAC
    TTCCTAACGG ATCTGGTGAT GTCTGAGGTG GATCGCTGCG GGGAACACGA TGTCTTGATC
    TTCAGGGAGA ACACACTTGC TACCAAAGCT ATTGAGGAAT ACCTGAAGTT GGTAGGGCAG
    AAGTATCTCC ATGATGCGCT AGGGGAGTTT ATCAAGGCTT TGTATGAGTC AGATGAAAAC
    TGTGAAGTGG ACCCCAGCAA ATGTTCATCC AGTGAATTAA CAGACCATCA GAGCAACCTG
    AAAATGTGTT GTGAGCTGGC CTTCTGCAAG ATTATCAATT CCTACTGCGT GTTCCCTCGT
    GAGCTGAAGG AGGTGTTTGC ATCTTGGAAG CAGCAGTGCC TGAGCAGGGG CAAGCAGGAC
    ATCAGCGAGC GGCTGATCAG TGCCTCCCTG TTCCTCCGCT TCCTGTGCCC CGCCATCATG
    TCCCCCAGCC TCTTCAGCCT CATGCAGGAG TATCCTGACG ACCGGACATC GCGCACGCTC
    ACACTCATTG CTAAAGTCAT TCAGAATCTC GCCAATTTTG CTAAGTTTGG TAACAAGGAA
    GAGTACATGG CTTTCATGAA CGACTTTCTG GAACATGAGT GGGGGGGAAT GAAGCGATTT
    CTCCTGGAGA TCTCTAATCC AGATACCATC TCAAACATGC CTGGATTTGA GGGCTATATC
    GACCTGGGCA GAGAGCTCTC AGTTTTGCAT TCCCTCTTAT GGGAGGTTGT GTCCCAACTT
    GATAAGGCGA CCGTGGCAAA ACTGGGACCT CTTCCTCGTA TTCTTGCCGA CATCACCAAA
    TCAATGACCA ACCCTACGCC AATACAGCAG CAGCTGAGGC GTTTCACTGA GCACAGCTCT
    AGTCCAAACG TCAGTGGCAG TCTCTCCTCA GGGCTTCAGA AGATATTCGA GGACCCCACT
    GATGGTGACT TGCATAAACT GAAGTCTCCA ACCCAGGAAA ACATAGATGG CTATTTCAGG
    GGCAAGACCT TGCTGTTGGT TCAGCAGGCG TCCACCCAGA GCATGACTTA CTCAGATAAG
    GACGAAAGAG AGAGCATCTT GCCCAACGGA CGTAGCATCT CCCTCATGGA CCTGCAGGAT
    CCCCATACCG CTCACAGCGA CCACGCTTCC ATGATGCACG ACGTGCCTTT GCGCTTGGCC
    GGCAGCCAGC TCTCCATCAC CCAGGTGGCC AACATCAAGC AGCTGTGGGA GACCCAGAGC
    ACGCCCCAAA GCGCTCCCCA GGTGAGGAGG CCGCTGCACC CGGCTTTGAA CCAGCAGGGC
    AGCCTCCAGC CTCTGTCATT CCAGAACCCG GTGTACCACC TCAACAACCC ACCTGCGCCG
    TTGCCCAAGG CCTCCGTGGA CTCCAGCCTG GAGAACCTGA GCACCGCCAG CTCCCAGAGC
    CGAAGCAACA GCGAAGACTT CAAGCTCAGC GGGCCCAGCA ACAGCAGCAT GGAGGACTTC
    ACCAAGCGCA GCACGCAGAG CGAGGACTTC TCTAGGCGGC ACACGGTCCC GGACAAGCAC
    GTCCCCATCG CGTTGCCCCG CCAGAACAGC AGCAGCCAGG CACAGATCCG CAAAATGGAC
    CAGGCCGGGT TAGGGGCCAG GGCCAAAGCG CCGCAGTCGC TGCCGCACAG CGCGTCCCTG
    CGCAGCACGG GCAGCATGTC GGCCGTGTCG GGGGCCGTGG TGACTGAGCC CGTCCAGAAC
    GGCAGCCGCT CCCGGCAGCA GTCGTCCTCC TCCAGGGAGA GCCCTGTGCC GAAGGTGAGG
    GCCATTCAGA GGCAGCAAAC GCAGCAGGTC CAGTCTCCTG TGGACTCAGC CACAATGTCA
    CCAGTGGAAA GGACAGCAGC GTGGGTTCTC AATAACGGGC AGTACGAGGA GGTCGAGGAG
    GACACAGAGC AGAACCCAGA TGAAGCCAAG CATGCTGAGA AGTACGAACA AGAGATAGCA
    AAGCTGAAGG AACGCTTGAA AGTGTCGAGC CGCCGGCTGG AGGAGTACGA GCGGCGGCTC
    CTGGTGCAGG AGCAGCAAAT GCAGAAGCTG CTGATGGAGT ACAAGTCCAG GCTGGAGGAC
    AGCGAGGAGA GACTGCGCAG GCAACAGGAG GAGAAGGACA GCCAGATGAA AAGCATTATC
    AGCAGGCTCA TGGCAGTTGA AGAGGAACTG AAGAAAGATC ATGCAGAGAT GCAAGCTGTC
    ATCGATGCAA AGCAGAAAAT TATTGATGCA CAGGAGAAGC GGATCGTGTC CCTGGACTCT
    GCCAACACGC GGCTGATGAG TGCCCTGACG CAGGTGAAGG AGCGCTACAG CATGCAAGTC
    CGCAATGGCA TCTCCCCCAC CAACCCCACC AAGCTTTCCA TCACGGAGAA TGGTGAATTC
    AAAAACAGCA GCTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa152368
    Clone ID Related Accession (Same CDS sequence) XM_015290515.2
    Accession Version XM_015290515.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3717bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product ras GTPase-activating protein nGAP isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006095.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015290515.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCAAGCC TGGTGAGGAA GATGCTAGTG CAGACAAAGC CATGGAGCGA TGTAAAAGGA 
    CCACCTACGC ACAGGCTGTC CTGCGGCCAG TCCCCCTACA CCGAGACGAC AACATGGGAG
    AGGAAGTACT GCATCCTGAC TGACAGCCAG CTGGTGCTGC TCAACAGGGA GAAGGAGGTG
    CCCACCGAGG GGTTGCAGGA GCCGCAGGAT GCAACCAAAG GCCGCTGTCT GCGCCGCACG
    GTGAGCGTGC CCTCCGAGGG CCAGTTCCCC GAGTACCCAC CAGATGGGGC CGCCAAGCTG
    GAGGTCCCGG CAGAGAGATC TCCTCGCAGA CGGAGCATCT CTGGGACCAG CACATCTGAG
    AAAACCAACT CCATGGATGC TGCAAATACC TCTCCTTTCA AAGTATCGGG CTTCTTCAGT
    AAACGTCTGA AAGGGTCCAT CAAGAGGACA AAGAGTCAGT CAAAGCTGGA CAGGAACACA
    AGTTTCCGCC TCCCATCCCT CCGCAATGCT GATGACAGGT CACGTGGGCT GCCGAAGCTG
    AAGGAGTCCC GCTCTCATGA GTCCCTGCTG AGCCCCGGCA GCGCAGTGGA AGCCCTGGAC
    CTTGGGGCAG AAGAAAAGGT CTTTGTAAAG CCACTGCACA GCAGCATCCT CGGACAGGAC
    TTCTGCTTTG AGGTAACCTA CTCCAGCGGC AGTAAATGCT TCAGCTGTTC CTCTGCTGCA
    GAGAGGGATA AGTGGATGGA AAACCTACGC AGAACAGTGC AGCCGAATAA GGACAACTGC
    CGTCGAGCTG AAAATGTGCT CCGTCTCTGG ATCATTGAGG CAAAGGACCT GGCCCCCAAG
    AAGAAATACT TCTGTGAGCT GTGCCTTGAT GACACTCTCT TTGCCCGCAC CACCAGCAAA
    ACGAAAGCAG ATAACATTTT CTGGGGGGAG CACTTTGAGT TCTACGGCCT CCCAGCTCTT
    CACAGCATCA CGGTTCACAT CTACAAGGAT GTGGAGAAGA AAAAGAAAAA GGACAAAAAC
    AATTACGTCG GCCTGGTCAA CATTCCCATG GCCACTGTAA CGGGGCGCCA GTTTGTGGAA
    AAATGGTACC CAGTCAGCAC ACCTACACCC AACAAAGGGA AATCAGGAGG CCCTTCCATT
    CGCATCAAGT CGCGTTATCA GACCATCACC ATCTTACCCA TGGAGCAATA CAAGGAGTTT
    GCAGAGTTTG TTACCAGCAA CTACACGATG CTGTGCTCTG TGCTGGAGCC AGTCATCAGC
    GTAAGGAATA AGGAAGAGAT GGCTTGTGCA ATGGTGCACA TTCTTCAGAG CACGGGGAGA
    GCCAAGGACT TCCTAACGGA TCTGGTGATG TCTGAGGTGG ATCGCTGCGG GGAACACGAT
    GTCTTGATCT TCAGGGAGAA CACACTTGCT ACCAAAGCTA TTGAGGAATA CCTGAAGTTG
    GTAGGGCAGA AGTATCTCCA TGATGCGCTA GGGGAGTTTA TCAAGGCTTT GTATGAGTCA
    GATGAAAACT GTGAAGTGGA CCCCAGCAAA TGTTCATCCA GTGAATTAAC AGACCATCAG
    AGCAACCTGA AAATGTGTTG TGAGCTGGCC TTCTGCAAGA TTATCAATTC CTACTGCGTG
    TTCCCTCGTG AGCTGAAGGA GGTGTTTGCA TCTTGGAAGC AGCAGTGCCT GAGCAGGGGC
    AAGCAGGACA TCAGCGAGCG GCTGATCAGT GCCTCCCTGT TCCTCCGCTT CCTGTGCCCC
    GCCATCATGT CCCCCAGCCT CTTCAGCCTC ATGCAGGAGT ATCCTGACGA CCGGACATCG
    CGCACGCTCA CACTCATTGC TAAAGTCATT CAGAATCTCG CCAATTTTGC TAAGTTTGGT
    AACAAGGAAG AGTACATGGC TTTCATGAAC GACTTTCTGG AACATGAGTG GGGGGGAATG
    AAGCGATTTC TCCTGGAGAT CTCTAATCCA GATACCATCT CAAACATGCC TGGATTTGAG
    GGCTATATCG ACCTGGGCAG AGAGCTCTCA GTTTTGCATT CCCTCTTATG GGAGGTTGTG
    TCCCAACTTG ATAAGGGTGA AAATTCCTTC CTACAGGCGA CCGTGGCAAA ACTGGGACCT
    CTTCCTCGTA TTCTTGCCGA CATCACCAAA TCAATGACCA ACCCTACGCC AATACAGCAG
    CAGCTGAGGC GTTTCACTGA GCACAGCTCT AGTCCAAACG TCAGTGGCAG TCTCTCCTCA
    GGGCTTCAGA AGATATTCGA GGACCCCACT GATGGTGACT TGCATAAACT GAAGTCTCCA
    ACCCAGGAAA ACATAGATGG CTATTTCAGG GGCAAGACCT TGCTGTTGGT TCAGCAGGCG
    TCCACCCAGA GCATGACTTA CTCAGATAAG GACGAAAGAG AGAGCATCTT GCCCAACGGA
    CGTAGCATCT CCCTCATGGA CCTGCAGGAT CCCCATACCG CTCACAGCGA CCACGCTTCC
    ATGATGCACG ACGTGCCTTT GCGCTTGGCC GGCAGCCAGC TCTCCATCAC CCAGGTGGCC
    AACATCAAGC AGCTGTGGGA GACCCAGAGC ACGCCCCAAA GCGCTCCCCA GGTGAGGAGG
    CCGCTGCACC CGGCTTTGAA CCAGCAGGGC AGCCTCCAGC CTCTGTCATT CCAGAACCCG
    GTGTACCACC TCAACAACCC ACCTGCGCCG TTGCCCAAGG CCTCCGTGGA CTCCAGCCTG
    GAGAACCTGA GCACCGCCAG CTCCCAGAGC CGAAGCAACA GCGAAGACTT CAAGCTCAGC
    GGGCCCAGCA ACAGCAGCAT GGAGGACTTC ACCAAGCGCA GCACGCAGAG CGAGGACTTC
    TCTAGGCGGC ACACGGTCCC GGACAAGCAC GTCCCCATCG CGTTGCCCCG CCAGAACAGC
    AGCAGCCAGG CACAGATCCG CAAAATGGAC CAGGCCGGGT TAGGGGCCAG GGCCAAAGCG
    CCGCAGTCGC TGCCGCACAG CGCGTCCCTG CGCAGCACGG GCAGCATGTC GGCCGTGTCG
    GGGGCCGTGG TGACTGAGCC CGTCCAGAAC GGCAGCCGCT CCCGGCAGCA GTCGTCCTCC
    TCCAGGGAGA GCCCTGTGCC GAAGGTGAGG GCCATTCAGA GGCAGCAAAC GCAGCAGGTC
    CAGTCTCCTG TGGACTCAGC CACAATGTCA CCAGTGGAAA GGACAGCAGC GTGGGTTCTC
    AATAACGGGC AGTACGAGGA GGTCGAGGAG GACACAGAGC AGAACCCAGA TGAAGCCAAG
    CATGCTGAGA AGTACGAACA AGAGATAGCA AAGCTGAAGG AACGCTTGAA AGTGTCGAGC
    CGCCGGCTGG AGGAGTACGA GCGGCGGCTC CTGGTGCAGG AGCAGCAAAT GCAGAAGCTG
    CTGATGGAGT ACAAGTCCAG GCTGGAGGAC AGCGAGGAGA GACTGCGCAG GCAACAGGAG
    GAGAAGGACA GCCAGATGAA AAGCATTATC AGCAGGCTCA TGGCAGTTGA AGAGGAACTG
    AAGAAAGATC ATGCAGAGAT GCAAGCTGTC ATCGATGCAA AGCAGAAAAT TATTGATGCA
    CAGGTCATTA ATGGGAATGA GATAATTCAA ACAGGAGAAG CGGATCGTGT CCCTGGACTC
    TGCCAACACG CGGCTGATGA GTGCCCTGAC GCAGGTGAAG GAGCGCTACA GCATGCAAGT
    CCGCAATGGC ATCTCCCCCA CCAACCCCAC CAAGCTTTCC ATCACGGAGA ATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015146001.1
    CDS17170..20886
    Translation

    Target ORF information:

    RefSeq Version XM_015290515.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015290515.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCAAGCC TGGTGAGGAA GATGCTAGTG CAGACAAAGC CATGGAGCGA TGTAAAAGGA 
    CCACCTACGC ACAGGCTGTC CTGCGGCCAG TCCCCCTACA CCGAGACGAC AACATGGGAG
    AGGAAGTACT GCATCCTGAC TGACAGCCAG CTGGTGCTGC TCAACAGGGA GAAGGAGGTG
    CCCACCGAGG GGTTGCAGGA GCCGCAGGAT GCAACCAAAG GCCGCTGTCT GCGCCGCACG
    GTGAGCGTGC CCTCCGAGGG CCAGTTCCCC GAGTACCCAC CAGATGGGGC CGCCAAGCTG
    GAGGTCCCGG CAGAGAGATC TCCTCGCAGA CGGAGCATCT CTGGGACCAG CACATCTGAG
    AAAACCAACT CCATGGATGC TGCAAATACC TCTCCTTTCA AAGTATCGGG CTTCTTCAGT
    AAACGTCTGA AAGGGTCCAT CAAGAGGACA AAGAGTCAGT CAAAGCTGGA CAGGAACACA
    AGTTTCCGCC TCCCATCCCT CCGCAATGCT GATGACAGGT CACGTGGGCT GCCGAAGCTG
    AAGGAGTCCC GCTCTCATGA GTCCCTGCTG AGCCCCGGCA GCGCAGTGGA AGCCCTGGAC
    CTTGGGGCAG AAGAAAAGGT CTTTGTAAAG CCACTGCACA GCAGCATCCT CGGACAGGAC
    TTCTGCTTTG AGGTAACCTA CTCCAGCGGC AGTAAATGCT TCAGCTGTTC CTCTGCTGCA
    GAGAGGGATA AGTGGATGGA AAACCTACGC AGAACAGTGC AGCCGAATAA GGACAACTGC
    CGTCGAGCTG AAAATGTGCT CCGTCTCTGG ATCATTGAGG CAAAGGACCT GGCCCCCAAG
    AAGAAATACT TCTGTGAGCT GTGCCTTGAT GACACTCTCT TTGCCCGCAC CACCAGCAAA
    ACGAAAGCAG ATAACATTTT CTGGGGGGAG CACTTTGAGT TCTACGGCCT CCCAGCTCTT
    CACAGCATCA CGGTTCACAT CTACAAGGAT GTGGAGAAGA AAAAGAAAAA GGACAAAAAC
    AATTACGTCG GCCTGGTCAA CATTCCCATG GCCACTGTAA CGGGGCGCCA GTTTGTGGAA
    AAATGGTACC CAGTCAGCAC ACCTACACCC AACAAAGGGA AATCAGGAGG CCCTTCCATT
    CGCATCAAGT CGCGTTATCA GACCATCACC ATCTTACCCA TGGAGCAATA CAAGGAGTTT
    GCAGAGTTTG TTACCAGCAA CTACACGATG CTGTGCTCTG TGCTGGAGCC AGTCATCAGC
    GTAAGGAATA AGGAAGAGAT GGCTTGTGCA ATGGTGCACA TTCTTCAGAG CACGGGGAGA
    GCCAAGGACT TCCTAACGGA TCTGGTGATG TCTGAGGTGG ATCGCTGCGG GGAACACGAT
    GTCTTGATCT TCAGGGAGAA CACACTTGCT ACCAAAGCTA TTGAGGAATA CCTGAAGTTG
    GTAGGGCAGA AGTATCTCCA TGATGCGCTA GGGGAGTTTA TCAAGGCTTT GTATGAGTCA
    GATGAAAACT GTGAAGTGGA CCCCAGCAAA TGTTCATCCA GTGAATTAAC AGACCATCAG
    AGCAACCTGA AAATGTGTTG TGAGCTGGCC TTCTGCAAGA TTATCAATTC CTACTGCGTG
    TTCCCTCGTG AGCTGAAGGA GGTGTTTGCA TCTTGGAAGC AGCAGTGCCT GAGCAGGGGC
    AAGCAGGACA TCAGCGAGCG GCTGATCAGT GCCTCCCTGT TCCTCCGCTT CCTGTGCCCC
    GCCATCATGT CCCCCAGCCT CTTCAGCCTC ATGCAGGAGT ATCCTGACGA CCGGACATCG
    CGCACGCTCA CACTCATTGC TAAAGTCATT CAGAATCTCG CCAATTTTGC TAAGTTTGGT
    AACAAGGAAG AGTACATGGC TTTCATGAAC GACTTTCTGG AACATGAGTG GGGGGGAATG
    AAGCGATTTC TCCTGGAGAT CTCTAATCCA GATACCATCT CAAACATGCC TGGATTTGAG
    GGCTATATCG ACCTGGGCAG AGAGCTCTCA GTTTTGCATT CCCTCTTATG GGAGGTTGTG
    TCCCAACTTG ATAAGGGTGA AAATTCCTTC CTACAGGCGA CCGTGGCAAA ACTGGGACCT
    CTTCCTCGTA TTCTTGCCGA CATCACCAAA TCAATGACCA ACCCTACGCC AATACAGCAG
    CAGCTGAGGC GTTTCACTGA GCACAGCTCT AGTCCAAACG TCAGTGGCAG TCTCTCCTCA
    GGGCTTCAGA AGATATTCGA GGACCCCACT GATGGTGACT TGCATAAACT GAAGTCTCCA
    ACCCAGGAAA ACATAGATGG CTATTTCAGG GGCAAGACCT TGCTGTTGGT TCAGCAGGCG
    TCCACCCAGA GCATGACTTA CTCAGATAAG GACGAAAGAG AGAGCATCTT GCCCAACGGA
    CGTAGCATCT CCCTCATGGA CCTGCAGGAT CCCCATACCG CTCACAGCGA CCACGCTTCC
    ATGATGCACG ACGTGCCTTT GCGCTTGGCC GGCAGCCAGC TCTCCATCAC CCAGGTGGCC
    AACATCAAGC AGCTGTGGGA GACCCAGAGC ACGCCCCAAA GCGCTCCCCA GGTGAGGAGG
    CCGCTGCACC CGGCTTTGAA CCAGCAGGGC AGCCTCCAGC CTCTGTCATT CCAGAACCCG
    GTGTACCACC TCAACAACCC ACCTGCGCCG TTGCCCAAGG CCTCCGTGGA CTCCAGCCTG
    GAGAACCTGA GCACCGCCAG CTCCCAGAGC CGAAGCAACA GCGAAGACTT CAAGCTCAGC
    GGGCCCAGCA ACAGCAGCAT GGAGGACTTC ACCAAGCGCA GCACGCAGAG CGAGGACTTC
    TCTAGGCGGC ACACGGTCCC GGACAAGCAC GTCCCCATCG CGTTGCCCCG CCAGAACAGC
    AGCAGCCAGG CACAGATCCG CAAAATGGAC CAGGCCGGGT TAGGGGCCAG GGCCAAAGCG
    CCGCAGTCGC TGCCGCACAG CGCGTCCCTG CGCAGCACGG GCAGCATGTC GGCCGTGTCG
    GGGGCCGTGG TGACTGAGCC CGTCCAGAAC GGCAGCCGCT CCCGGCAGCA GTCGTCCTCC
    TCCAGGGAGA GCCCTGTGCC GAAGGTGAGG GCCATTCAGA GGCAGCAAAC GCAGCAGGTC
    CAGTCTCCTG TGGACTCAGC CACAATGTCA CCAGTGGAAA GGACAGCAGC GTGGGTTCTC
    AATAACGGGC AGTACGAGGA GGTCGAGGAG GACACAGAGC AGAACCCAGA TGAAGCCAAG
    CATGCTGAGA AGTACGAACA AGAGATAGCA AAGCTGAAGG AACGCTTGAA AGTGTCGAGC
    CGCCGGCTGG AGGAGTACGA GCGGCGGCTC CTGGTGCAGG AGCAGCAAAT GCAGAAGCTG
    CTGATGGAGT ACAAGTCCAG GCTGGAGGAC AGCGAGGAGA GACTGCGCAG GCAACAGGAG
    GAGAAGGACA GCCAGATGAA AAGCATTATC AGCAGGCTCA TGGCAGTTGA AGAGGAACTG
    AAGAAAGATC ATGCAGAGAT GCAAGCTGTC ATCGATGCAA AGCAGAAAAT TATTGATGCA
    CAGGTCATTA ATGGGAATGA GATAATTCAA ACAGGAGAAG CGGATCGTGT CCCTGGACTC
    TGCCAACACG CGGCTGATGA GTGCCCTGAC GCAGGTGAAG GAGCGCTACA GCATGCAAGT
    CCGCAATGGC ATCTCCCCCA CCAACCCCAC CAAGCTTTCC ATCACGGAGA ATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa152369
    Clone ID Related Accession (Same CDS sequence) XM_015290516.2
    Accession Version XM_015290516.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3498bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product ras GTPase-activating protein nGAP isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006095.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015290516.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGTCTCC TTTCTCCCGG CGATGTGGCC GAGTGGCTGG ATAGCAGCAC AGAAGATGAG 
    AAAAGCCTTC TGTCGTTTCT CGAGGTCCCG GCAGAGAGAT CTCCTCGCAG ACGGAGCATC
    TCTGGGACCA GCACATCTGA GAAAACCAAC TCCATGGATG CTGCAAATAC CTCTCCTTTC
    AAAGTATCGG GCTTCTTCAG TAAACGTCTG AAAGGGTCCA TCAAGAGGAC AAAGAGTCAG
    TCAAAGCTGG ACAGGAACAC AAGTTTCCGC CTCCCATCCC TCCGCAATGC TGATGACAGG
    TCACGTGGGC TGCCGAAGCT GAAGGAGTCC CGCTCTCATG AGTCCCTGCT GAGCCCCGGC
    AGCGCAGTGG AAGCCCTGGA CCTTGGGGCA GAAGAAAAGG TCTTTGTAAA GCCACTGCAC
    AGCAGCATCC TCGGACAGGA CTTCTGCTTT GAGGTAACCT ACTCCAGCGG CAGTAAATGC
    TTCAGCTGTT CCTCTGCTGC AGAGAGGGAT AAGTGGATGG AAAACCTACG CAGAACAGTG
    CAGCCGAATA AGGACAACTG CCGTCGAGCT GAAAATGTGC TCCGTCTCTG GATCATTGAG
    GCAAAGGACC TGGCCCCCAA GAAGAAATAC TTCTGTGAGC TGTGCCTTGA TGACACTCTC
    TTTGCCCGCA CCACCAGCAA AACGAAAGCA GATAACATTT TCTGGGGGGA GCACTTTGAG
    TTCTACGGCC TCCCAGCTCT TCACAGCATC ACGGTTCACA TCTACAAGGA TGTGGAGAAG
    AAAAAGAAAA AGGACAAAAA CAATTACGTC GGCCTGGTCA ACATTCCCAT GGCCACTGTA
    ACGGGGCGCC AGTTTGTGGA AAAATGGTAC CCAGTCAGCA CACCTACACC CAACAAAGGG
    AAATCAGGAG GCCCTTCCAT TCGCATCAAG TCGCGTTATC AGACCATCAC CATCTTACCC
    ATGGAGCAAT ACAAGGAGTT TGCAGAGTTT GTTACCAGCA ACTACACGAT GCTGTGCTCT
    GTGCTGGAGC CAGTCATCAG CGTAAGGAAT AAGGAAGAGA TGGCTTGTGC AATGGTGCAC
    ATTCTTCAGA GCACGGGGAG AGCCAAGGAC TTCCTAACGG ATCTGGTGAT GTCTGAGGTG
    GATCGCTGCG GGGAACACGA TGTCTTGATC TTCAGGGAGA ACACACTTGC TACCAAAGCT
    ATTGAGGAAT ACCTGAAGTT GGTAGGGCAG AAGTATCTCC ATGATGCGCT AGGGGAGTTT
    ATCAAGGCTT TGTATGAGTC AGATGAAAAC TGTGAAGTGG ACCCCAGCAA ATGTTCATCC
    AGTGAATTAA CAGACCATCA GAGCAACCTG AAAATGTGTT GTGAGCTGGC CTTCTGCAAG
    ATTATCAATT CCTACTGCGT GTTCCCTCGT GAGCTGAAGG AGGTGTTTGC ATCTTGGAAG
    CAGCAGTGCC TGAGCAGGGG CAAGCAGGAC ATCAGCGAGC GGCTGATCAG TGCCTCCCTG
    TTCCTCCGCT TCCTGTGCCC CGCCATCATG TCCCCCAGCC TCTTCAGCCT CATGCAGGAG
    TATCCTGACG ACCGGACATC GCGCACGCTC ACACTCATTG CTAAAGTCAT TCAGAATCTC
    GCCAATTTTG CTAAGTTTGG TAACAAGGAA GAGTACATGG CTTTCATGAA CGACTTTCTG
    GAACATGAGT GGGGGGGAAT GAAGCGATTT CTCCTGGAGA TCTCTAATCC AGATACCATC
    TCAAACATGC CTGGATTTGA GGGCTATATC GACCTGGGCA GAGAGCTCTC AGTTTTGCAT
    TCCCTCTTAT GGGAGGTTGT GTCCCAACTT GATAAGGGTG AAAATTCCTT CCTACAGGCG
    ACCGTGGCAA AACTGGGACC TCTTCCTCGT ATTCTTGCCG ACATCACCAA ATCAATGACC
    AACCCTACGC CAATACAGCA GCAGCTGAGG CGTTTCACTG AGCACAGCTC TAGTCCAAAC
    GTCAGTGGCA GTCTCTCCTC AGGGCTTCAG AAGATATTCG AGGACCCCAC TGATGGTGAC
    TTGCATAAAC TGAAGTCTCC AACCCAGGAA AACATAGATG GCTATTTCAG GGGCAAGACC
    TTGCTGTTGG TTCAGCAGGC GTCCACCCAG AGCATGACTT ACTCAGATAA GGACGAAAGA
    GAGAGCATCT TGCCCAACGG ACGTAGCATC TCCCTCATGG ACCTGCAGGA TCCCCATACC
    GCTCACAGCG ACCACGCTTC CATGATGCAC GACGTGCCTT TGCGCTTGGC CGGCAGCCAG
    CTCTCCATCA CCCAGGTGGC CAACATCAAG CAGCTGTGGG AGACCCAGAG CACGCCCCAA
    AGCGCTCCCC AGGTGAGGAG GCCGCTGCAC CCGGCTTTGA ACCAGCAGGG CAGCCTCCAG
    CCTCTGTCAT TCCAGAACCC GGTGTACCAC CTCAACAACC CACCTGCGCC GTTGCCCAAG
    GCCTCCGTGG ACTCCAGCCT GGAGAACCTG AGCACCGCCA GCTCCCAGAG CCGAAGCAAC
    AGCGAAGACT TCAAGCTCAG CGGGCCCAGC AACAGCAGCA TGGAGGACTT CACCAAGCGC
    AGCACGCAGA GCGAGGACTT CTCTAGGCGG CACACGGTCC CGGACAAGCA CGTCCCCATC
    GCGTTGCCCC GCCAGAACAG CAGCAGCCAG GCACAGATCC GCAAAATGGA CCAGGCCGGG
    TTAGGGGCCA GGGCCAAAGC GCCGCAGTCG CTGCCGCACA GCGCGTCCCT GCGCAGCACG
    GGCAGCATGT CGGCCGTGTC GGGGGCCGTG GTGACTGAGC CCGTCCAGAA CGGCAGCCGC
    TCCCGGCAGC AGTCGTCCTC CTCCAGGGAG AGCCCTGTGC CGAAGGTGAG GGCCATTCAG
    AGGCAGCAAA CGCAGCAGGT CCAGTCTCCT GTGGACTCAG CCACAATGTC ACCAGTGGAA
    AGGACAGCAG CGTGGGTTCT CAATAACGGG CAGTACGAGG AGGTCGAGGA GGACACAGAG
    CAGAACCCAG ATGAAGCCAA GCATGCTGAG AAGTACGAAC AAGAGATAGC AAAGCTGAAG
    GAACGCTTGA AAGTGTCGAG CCGCCGGCTG GAGGAGTACG AGCGGCGGCT CCTGGTGCAG
    GAGCAGCAAA TGCAGAAGCT GCTGATGGAG TACAAGTCCA GGCTGGAGGA CAGCGAGGAG
    AGACTGCGCA GGCAACAGGA GGAGAAGGAC AGCCAGATGA AAAGCATTAT CAGCAGGCTC
    ATGGCAGTTG AAGAGGAACT GAAGAAAGAT CATGCAGAGA TGCAAGCTGT CATCGATGCA
    AAGCAGAAAA TTATTGATGC ACAGGTCATT AATGGGAATG AGATAATTCA AACAGGAGAA
    GCGGATCGTG TCCCTGGACT CTGCCAACAC GCGGCTGATG AGTGCCCTGA CGCAGGTGAA
    GGAGCGCTAC AGCATGCAAG TCCGCAATGG CATCTCCCCC ACCAACCCCA CCAAGCTTTC
    CATCACGGAG AATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015146002.1
    CDS16..3513
    Translation

    Target ORF information:

    RefSeq Version XM_015290516.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015290516.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGTCTCC TTTCTCCCGG CGATGTGGCC GAGTGGCTGG ATAGCAGCAC AGAAGATGAG 
    AAAAGCCTTC TGTCGTTTCT CGAGGTCCCG GCAGAGAGAT CTCCTCGCAG ACGGAGCATC
    TCTGGGACCA GCACATCTGA GAAAACCAAC TCCATGGATG CTGCAAATAC CTCTCCTTTC
    AAAGTATCGG GCTTCTTCAG TAAACGTCTG AAAGGGTCCA TCAAGAGGAC AAAGAGTCAG
    TCAAAGCTGG ACAGGAACAC AAGTTTCCGC CTCCCATCCC TCCGCAATGC TGATGACAGG
    TCACGTGGGC TGCCGAAGCT GAAGGAGTCC CGCTCTCATG AGTCCCTGCT GAGCCCCGGC
    AGCGCAGTGG AAGCCCTGGA CCTTGGGGCA GAAGAAAAGG TCTTTGTAAA GCCACTGCAC
    AGCAGCATCC TCGGACAGGA CTTCTGCTTT GAGGTAACCT ACTCCAGCGG CAGTAAATGC
    TTCAGCTGTT CCTCTGCTGC AGAGAGGGAT AAGTGGATGG AAAACCTACG CAGAACAGTG
    CAGCCGAATA AGGACAACTG CCGTCGAGCT GAAAATGTGC TCCGTCTCTG GATCATTGAG
    GCAAAGGACC TGGCCCCCAA GAAGAAATAC TTCTGTGAGC TGTGCCTTGA TGACACTCTC
    TTTGCCCGCA CCACCAGCAA AACGAAAGCA GATAACATTT TCTGGGGGGA GCACTTTGAG
    TTCTACGGCC TCCCAGCTCT TCACAGCATC ACGGTTCACA TCTACAAGGA TGTGGAGAAG
    AAAAAGAAAA AGGACAAAAA CAATTACGTC GGCCTGGTCA ACATTCCCAT GGCCACTGTA
    ACGGGGCGCC AGTTTGTGGA AAAATGGTAC CCAGTCAGCA CACCTACACC CAACAAAGGG
    AAATCAGGAG GCCCTTCCAT TCGCATCAAG TCGCGTTATC AGACCATCAC CATCTTACCC
    ATGGAGCAAT ACAAGGAGTT TGCAGAGTTT GTTACCAGCA ACTACACGAT GCTGTGCTCT
    GTGCTGGAGC CAGTCATCAG CGTAAGGAAT AAGGAAGAGA TGGCTTGTGC AATGGTGCAC
    ATTCTTCAGA GCACGGGGAG AGCCAAGGAC TTCCTAACGG ATCTGGTGAT GTCTGAGGTG
    GATCGCTGCG GGGAACACGA TGTCTTGATC TTCAGGGAGA ACACACTTGC TACCAAAGCT
    ATTGAGGAAT ACCTGAAGTT GGTAGGGCAG AAGTATCTCC ATGATGCGCT AGGGGAGTTT
    ATCAAGGCTT TGTATGAGTC AGATGAAAAC TGTGAAGTGG ACCCCAGCAA ATGTTCATCC
    AGTGAATTAA CAGACCATCA GAGCAACCTG AAAATGTGTT GTGAGCTGGC CTTCTGCAAG
    ATTATCAATT CCTACTGCGT GTTCCCTCGT GAGCTGAAGG AGGTGTTTGC ATCTTGGAAG
    CAGCAGTGCC TGAGCAGGGG CAAGCAGGAC ATCAGCGAGC GGCTGATCAG TGCCTCCCTG
    TTCCTCCGCT TCCTGTGCCC CGCCATCATG TCCCCCAGCC TCTTCAGCCT CATGCAGGAG
    TATCCTGACG ACCGGACATC GCGCACGCTC ACACTCATTG CTAAAGTCAT TCAGAATCTC
    GCCAATTTTG CTAAGTTTGG TAACAAGGAA GAGTACATGG CTTTCATGAA CGACTTTCTG
    GAACATGAGT GGGGGGGAAT GAAGCGATTT CTCCTGGAGA TCTCTAATCC AGATACCATC
    TCAAACATGC CTGGATTTGA GGGCTATATC GACCTGGGCA GAGAGCTCTC AGTTTTGCAT
    TCCCTCTTAT GGGAGGTTGT GTCCCAACTT GATAAGGGTG AAAATTCCTT CCTACAGGCG
    ACCGTGGCAA AACTGGGACC TCTTCCTCGT ATTCTTGCCG ACATCACCAA ATCAATGACC
    AACCCTACGC CAATACAGCA GCAGCTGAGG CGTTTCACTG AGCACAGCTC TAGTCCAAAC
    GTCAGTGGCA GTCTCTCCTC AGGGCTTCAG AAGATATTCG AGGACCCCAC TGATGGTGAC
    TTGCATAAAC TGAAGTCTCC AACCCAGGAA AACATAGATG GCTATTTCAG GGGCAAGACC
    TTGCTGTTGG TTCAGCAGGC GTCCACCCAG AGCATGACTT ACTCAGATAA GGACGAAAGA
    GAGAGCATCT TGCCCAACGG ACGTAGCATC TCCCTCATGG ACCTGCAGGA TCCCCATACC
    GCTCACAGCG ACCACGCTTC CATGATGCAC GACGTGCCTT TGCGCTTGGC CGGCAGCCAG
    CTCTCCATCA CCCAGGTGGC CAACATCAAG CAGCTGTGGG AGACCCAGAG CACGCCCCAA
    AGCGCTCCCC AGGTGAGGAG GCCGCTGCAC CCGGCTTTGA ACCAGCAGGG CAGCCTCCAG
    CCTCTGTCAT TCCAGAACCC GGTGTACCAC CTCAACAACC CACCTGCGCC GTTGCCCAAG
    GCCTCCGTGG ACTCCAGCCT GGAGAACCTG AGCACCGCCA GCTCCCAGAG CCGAAGCAAC
    AGCGAAGACT TCAAGCTCAG CGGGCCCAGC AACAGCAGCA TGGAGGACTT CACCAAGCGC
    AGCACGCAGA GCGAGGACTT CTCTAGGCGG CACACGGTCC CGGACAAGCA CGTCCCCATC
    GCGTTGCCCC GCCAGAACAG CAGCAGCCAG GCACAGATCC GCAAAATGGA CCAGGCCGGG
    TTAGGGGCCA GGGCCAAAGC GCCGCAGTCG CTGCCGCACA GCGCGTCCCT GCGCAGCACG
    GGCAGCATGT CGGCCGTGTC GGGGGCCGTG GTGACTGAGC CCGTCCAGAA CGGCAGCCGC
    TCCCGGCAGC AGTCGTCCTC CTCCAGGGAG AGCCCTGTGC CGAAGGTGAG GGCCATTCAG
    AGGCAGCAAA CGCAGCAGGT CCAGTCTCCT GTGGACTCAG CCACAATGTC ACCAGTGGAA
    AGGACAGCAG CGTGGGTTCT CAATAACGGG CAGTACGAGG AGGTCGAGGA GGACACAGAG
    CAGAACCCAG ATGAAGCCAA GCATGCTGAG AAGTACGAAC AAGAGATAGC AAAGCTGAAG
    GAACGCTTGA AAGTGTCGAG CCGCCGGCTG GAGGAGTACG AGCGGCGGCT CCTGGTGCAG
    GAGCAGCAAA TGCAGAAGCT GCTGATGGAG TACAAGTCCA GGCTGGAGGA CAGCGAGGAG
    AGACTGCGCA GGCAACAGGA GGAGAAGGAC AGCCAGATGA AAAGCATTAT CAGCAGGCTC
    ATGGCAGTTG AAGAGGAACT GAAGAAAGAT CATGCAGAGA TGCAAGCTGT CATCGATGCA
    AAGCAGAAAA TTATTGATGC ACAGGTCATT AATGGGAATG AGATAATTCA AACAGGAGAA
    GCGGATCGTG TCCCTGGACT CTGCCAACAC GCGGCTGATG AGTGCCCTGA CGCAGGTGAA
    GGAGCGCTAC AGCATGCAAG TCCGCAATGG CATCTCCCCC ACCAACCCCA CCAAGCTTTC
    CATCACGGAG AATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa152370
    Clone ID Related Accession (Same CDS sequence) XM_004943254.3
    Accession Version XM_004943254.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3438bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product ras GTPase-activating protein nGAP isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006095.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004943254.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGGCAGCA CTTGTGACAA AGAGGTCCCG GCAGAGAGAT CTCCTCGCAG ACGGAGCATC 
    TCTGGGACCA GCACATCTGA GAAAACCAAC TCCATGGATG CTGCAAATAC CTCTCCTTTC
    AAAGTATCGG GCTTCTTCAG TAAACGTCTG AAAGGGTCCA TCAAGAGGAC AAAGAGTCAG
    TCAAAGCTGG ACAGGAACAC AAGTTTCCGC CTCCCATCCC TCCGCAATGC TGATGACAGG
    TCACGTGGGC TGCCGAAGCT GAAGGAGTCC CGCTCTCATG AGTCCCTGCT GAGCCCCGGC
    AGCGCAGTGG AAGCCCTGGA CCTTGGGGCA GAAGAAAAGG TCTTTGTAAA GCCACTGCAC
    AGCAGCATCC TCGGACAGGA CTTCTGCTTT GAGGTAACCT ACTCCAGCGG CAGTAAATGC
    TTCAGCTGTT CCTCTGCTGC AGAGAGGGAT AAGTGGATGG AAAACCTACG CAGAACAGTG
    CAGCCGAATA AGGACAACTG CCGTCGAGCT GAAAATGTGC TCCGTCTCTG GATCATTGAG
    GCAAAGGACC TGGCCCCCAA GAAGAAATAC TTCTGTGAGC TGTGCCTTGA TGACACTCTC
    TTTGCCCGCA CCACCAGCAA AACGAAAGCA GATAACATTT TCTGGGGGGA GCACTTTGAG
    TTCTACGGCC TCCCAGCTCT TCACAGCATC ACGGTTCACA TCTACAAGGA TGTGGAGAAG
    AAAAAGAAAA AGGACAAAAA CAATTACGTC GGCCTGGTCA ACATTCCCAT GGCCACTGTA
    ACGGGGCGCC AGTTTGTGGA AAAATGGTAC CCAGTCAGCA CACCTACACC CAACAAAGGG
    AAATCAGGAG GCCCTTCCAT TCGCATCAAG TCGCGTTATC AGACCATCAC CATCTTACCC
    ATGGAGCAAT ACAAGGAGTT TGCAGAGTTT GTTACCAGCA ACTACACGAT GCTGTGCTCT
    GTGCTGGAGC CAGTCATCAG CGTAAGGAAT AAGGAAGAGA TGGCTTGTGC AATGGTGCAC
    ATTCTTCAGA GCACGGGGAG AGCCAAGGAC TTCCTAACGG ATCTGGTGAT GTCTGAGGTG
    GATCGCTGCG GGGAACACGA TGTCTTGATC TTCAGGGAGA ACACACTTGC TACCAAAGCT
    ATTGAGGAAT ACCTGAAGTT GGTAGGGCAG AAGTATCTCC ATGATGCGCT AGGGGAGTTT
    ATCAAGGCTT TGTATGAGTC AGATGAAAAC TGTGAAGTGG ACCCCAGCAA ATGTTCATCC
    AGTGAATTAA CAGACCATCA GAGCAACCTG AAAATGTGTT GTGAGCTGGC CTTCTGCAAG
    ATTATCAATT CCTACTGCGT GTTCCCTCGT GAGCTGAAGG AGGTGTTTGC ATCTTGGAAG
    CAGCAGTGCC TGAGCAGGGG CAAGCAGGAC ATCAGCGAGC GGCTGATCAG TGCCTCCCTG
    TTCCTCCGCT TCCTGTGCCC CGCCATCATG TCCCCCAGCC TCTTCAGCCT CATGCAGGAG
    TATCCTGACG ACCGGACATC GCGCACGCTC ACACTCATTG CTAAAGTCAT TCAGAATCTC
    GCCAATTTTG CTAAGTTTGG TAACAAGGAA GAGTACATGG CTTTCATGAA CGACTTTCTG
    GAACATGAGT GGGGGGGAAT GAAGCGATTT CTCCTGGAGA TCTCTAATCC AGATACCATC
    TCAAACATGC CTGGATTTGA GGGCTATATC GACCTGGGCA GAGAGCTCTC AGTTTTGCAT
    TCCCTCTTAT GGGAGGTTGT GTCCCAACTT GATAAGGGTG AAAATTCCTT CCTACAGGCG
    ACCGTGGCAA AACTGGGACC TCTTCCTCGT ATTCTTGCCG ACATCACCAA ATCAATGACC
    AACCCTACGC CAATACAGCA GCAGCTGAGG CGTTTCACTG AGCACAGCTC TAGTCCAAAC
    GTCAGTGGCA GTCTCTCCTC AGGGCTTCAG AAGATATTCG AGGACCCCAC TGATGGTGAC
    TTGCATAAAC TGAAGTCTCC AACCCAGGAA AACATAGATG GCTATTTCAG GGGCAAGACC
    TTGCTGTTGG TTCAGCAGGC GTCCACCCAG AGCATGACTT ACTCAGATAA GGACGAAAGA
    GAGAGCATCT TGCCCAACGG ACGTAGCATC TCCCTCATGG ACCTGCAGGA TCCCCATACC
    GCTCACAGCG ACCACGCTTC CATGATGCAC GACGTGCCTT TGCGCTTGGC CGGCAGCCAG
    CTCTCCATCA CCCAGGTGGC CAACATCAAG CAGCTGTGGG AGACCCAGAG CACGCCCCAA
    AGCGCTCCCC AGGTGAGGAG GCCGCTGCAC CCGGCTTTGA ACCAGCAGGG CAGCCTCCAG
    CCTCTGTCAT TCCAGAACCC GGTGTACCAC CTCAACAACC CACCTGCGCC GTTGCCCAAG
    GCCTCCGTGG ACTCCAGCCT GGAGAACCTG AGCACCGCCA GCTCCCAGAG CCGAAGCAAC
    AGCGAAGACT TCAAGCTCAG CGGGCCCAGC AACAGCAGCA TGGAGGACTT CACCAAGCGC
    AGCACGCAGA GCGAGGACTT CTCTAGGCGG CACACGGTCC CGGACAAGCA CGTCCCCATC
    GCGTTGCCCC GCCAGAACAG CAGCAGCCAG GCACAGATCC GCAAAATGGA CCAGGCCGGG
    TTAGGGGCCA GGGCCAAAGC GCCGCAGTCG CTGCCGCACA GCGCGTCCCT GCGCAGCACG
    GGCAGCATGT CGGCCGTGTC GGGGGCCGTG GTGACTGAGC CCGTCCAGAA CGGCAGCCGC
    TCCCGGCAGC AGTCGTCCTC CTCCAGGGAG AGCCCTGTGC CGAAGGTGAG GGCCATTCAG
    AGGCAGCAAA CGCAGCAGGT CCAGTCTCCT GTGGACTCAG CCACAATGTC ACCAGTGGAA
    AGGACAGCAG CGTGGGTTCT CAATAACGGG CAGTACGAGG AGGTCGAGGA GGACACAGAG
    CAGAACCCAG ATGAAGCCAA GCATGCTGAG AAGTACGAAC AAGAGATAGC AAAGCTGAAG
    GAACGCTTGA AAGTGTCGAG CCGCCGGCTG GAGGAGTACG AGCGGCGGCT CCTGGTGCAG
    GAGCAGCAAA TGCAGAAGCT GCTGATGGAG TACAAGTCCA GGCTGGAGGA CAGCGAGGAG
    AGACTGCGCA GGCAACAGGA GGAGAAGGAC AGCCAGATGA AAAGCATTAT CAGCAGGCTC
    ATGGCAGTTG AAGAGGAACT GAAGAAAGAT CATGCAGAGA TGCAAGCTGT CATCGATGCA
    AAGCAGAAAA TTATTGATGC ACAGGTCATT AATGGGAATG AGATAATTCA AACAGGAGAA
    GCGGATCGTG TCCCTGGACT CTGCCAACAC GCGGCTGATG AGTGCCCTGA CGCAGGTGAA
    GGAGCGCTAC AGCATGCAAG TCCGCAATGG CATCTCCCCC ACCAACCCCA CCAAGCTTTC
    CATCACGGAG AATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004943311.2
    CDS757..4194
    Translation

    Target ORF information:

    RefSeq Version XM_004943254.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004943254.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGGCAGCA CTTGTGACAA AGAGGTCCCG GCAGAGAGAT CTCCTCGCAG ACGGAGCATC 
    TCTGGGACCA GCACATCTGA GAAAACCAAC TCCATGGATG CTGCAAATAC CTCTCCTTTC
    AAAGTATCGG GCTTCTTCAG TAAACGTCTG AAAGGGTCCA TCAAGAGGAC AAAGAGTCAG
    TCAAAGCTGG ACAGGAACAC AAGTTTCCGC CTCCCATCCC TCCGCAATGC TGATGACAGG
    TCACGTGGGC TGCCGAAGCT GAAGGAGTCC CGCTCTCATG AGTCCCTGCT GAGCCCCGGC
    AGCGCAGTGG AAGCCCTGGA CCTTGGGGCA GAAGAAAAGG TCTTTGTAAA GCCACTGCAC
    AGCAGCATCC TCGGACAGGA CTTCTGCTTT GAGGTAACCT ACTCCAGCGG CAGTAAATGC
    TTCAGCTGTT CCTCTGCTGC AGAGAGGGAT AAGTGGATGG AAAACCTACG CAGAACAGTG
    CAGCCGAATA AGGACAACTG CCGTCGAGCT GAAAATGTGC TCCGTCTCTG GATCATTGAG
    GCAAAGGACC TGGCCCCCAA GAAGAAATAC TTCTGTGAGC TGTGCCTTGA TGACACTCTC
    TTTGCCCGCA CCACCAGCAA AACGAAAGCA GATAACATTT TCTGGGGGGA GCACTTTGAG
    TTCTACGGCC TCCCAGCTCT TCACAGCATC ACGGTTCACA TCTACAAGGA TGTGGAGAAG
    AAAAAGAAAA AGGACAAAAA CAATTACGTC GGCCTGGTCA ACATTCCCAT GGCCACTGTA
    ACGGGGCGCC AGTTTGTGGA AAAATGGTAC CCAGTCAGCA CACCTACACC CAACAAAGGG
    AAATCAGGAG GCCCTTCCAT TCGCATCAAG TCGCGTTATC AGACCATCAC CATCTTACCC
    ATGGAGCAAT ACAAGGAGTT TGCAGAGTTT GTTACCAGCA ACTACACGAT GCTGTGCTCT
    GTGCTGGAGC CAGTCATCAG CGTAAGGAAT AAGGAAGAGA TGGCTTGTGC AATGGTGCAC
    ATTCTTCAGA GCACGGGGAG AGCCAAGGAC TTCCTAACGG ATCTGGTGAT GTCTGAGGTG
    GATCGCTGCG GGGAACACGA TGTCTTGATC TTCAGGGAGA ACACACTTGC TACCAAAGCT
    ATTGAGGAAT ACCTGAAGTT GGTAGGGCAG AAGTATCTCC ATGATGCGCT AGGGGAGTTT
    ATCAAGGCTT TGTATGAGTC AGATGAAAAC TGTGAAGTGG ACCCCAGCAA ATGTTCATCC
    AGTGAATTAA CAGACCATCA GAGCAACCTG AAAATGTGTT GTGAGCTGGC CTTCTGCAAG
    ATTATCAATT CCTACTGCGT GTTCCCTCGT GAGCTGAAGG AGGTGTTTGC ATCTTGGAAG
    CAGCAGTGCC TGAGCAGGGG CAAGCAGGAC ATCAGCGAGC GGCTGATCAG TGCCTCCCTG
    TTCCTCCGCT TCCTGTGCCC CGCCATCATG TCCCCCAGCC TCTTCAGCCT CATGCAGGAG
    TATCCTGACG ACCGGACATC GCGCACGCTC ACACTCATTG CTAAAGTCAT TCAGAATCTC
    GCCAATTTTG CTAAGTTTGG TAACAAGGAA GAGTACATGG CTTTCATGAA CGACTTTCTG
    GAACATGAGT GGGGGGGAAT GAAGCGATTT CTCCTGGAGA TCTCTAATCC AGATACCATC
    TCAAACATGC CTGGATTTGA GGGCTATATC GACCTGGGCA GAGAGCTCTC AGTTTTGCAT
    TCCCTCTTAT GGGAGGTTGT GTCCCAACTT GATAAGGGTG AAAATTCCTT CCTACAGGCG
    ACCGTGGCAA AACTGGGACC TCTTCCTCGT ATTCTTGCCG ACATCACCAA ATCAATGACC
    AACCCTACGC CAATACAGCA GCAGCTGAGG CGTTTCACTG AGCACAGCTC TAGTCCAAAC
    GTCAGTGGCA GTCTCTCCTC AGGGCTTCAG AAGATATTCG AGGACCCCAC TGATGGTGAC
    TTGCATAAAC TGAAGTCTCC AACCCAGGAA AACATAGATG GCTATTTCAG GGGCAAGACC
    TTGCTGTTGG TTCAGCAGGC GTCCACCCAG AGCATGACTT ACTCAGATAA GGACGAAAGA
    GAGAGCATCT TGCCCAACGG ACGTAGCATC TCCCTCATGG ACCTGCAGGA TCCCCATACC
    GCTCACAGCG ACCACGCTTC CATGATGCAC GACGTGCCTT TGCGCTTGGC CGGCAGCCAG
    CTCTCCATCA CCCAGGTGGC CAACATCAAG CAGCTGTGGG AGACCCAGAG CACGCCCCAA
    AGCGCTCCCC AGGTGAGGAG GCCGCTGCAC CCGGCTTTGA ACCAGCAGGG CAGCCTCCAG
    CCTCTGTCAT TCCAGAACCC GGTGTACCAC CTCAACAACC CACCTGCGCC GTTGCCCAAG
    GCCTCCGTGG ACTCCAGCCT GGAGAACCTG AGCACCGCCA GCTCCCAGAG CCGAAGCAAC
    AGCGAAGACT TCAAGCTCAG CGGGCCCAGC AACAGCAGCA TGGAGGACTT CACCAAGCGC
    AGCACGCAGA GCGAGGACTT CTCTAGGCGG CACACGGTCC CGGACAAGCA CGTCCCCATC
    GCGTTGCCCC GCCAGAACAG CAGCAGCCAG GCACAGATCC GCAAAATGGA CCAGGCCGGG
    TTAGGGGCCA GGGCCAAAGC GCCGCAGTCG CTGCCGCACA GCGCGTCCCT GCGCAGCACG
    GGCAGCATGT CGGCCGTGTC GGGGGCCGTG GTGACTGAGC CCGTCCAGAA CGGCAGCCGC
    TCCCGGCAGC AGTCGTCCTC CTCCAGGGAG AGCCCTGTGC CGAAGGTGAG GGCCATTCAG
    AGGCAGCAAA CGCAGCAGGT CCAGTCTCCT GTGGACTCAG CCACAATGTC ACCAGTGGAA
    AGGACAGCAG CGTGGGTTCT CAATAACGGG CAGTACGAGG AGGTCGAGGA GGACACAGAG
    CAGAACCCAG ATGAAGCCAA GCATGCTGAG AAGTACGAAC AAGAGATAGC AAAGCTGAAG
    GAACGCTTGA AAGTGTCGAG CCGCCGGCTG GAGGAGTACG AGCGGCGGCT CCTGGTGCAG
    GAGCAGCAAA TGCAGAAGCT GCTGATGGAG TACAAGTCCA GGCTGGAGGA CAGCGAGGAG
    AGACTGCGCA GGCAACAGGA GGAGAAGGAC AGCCAGATGA AAAGCATTAT CAGCAGGCTC
    ATGGCAGTTG AAGAGGAACT GAAGAAAGAT CATGCAGAGA TGCAAGCTGT CATCGATGCA
    AAGCAGAAAA TTATTGATGC ACAGGTCATT AATGGGAATG AGATAATTCA AACAGGAGAA
    GCGGATCGTG TCCCTGGACT CTGCCAACAC GCGGCTGATG AGTGCCCTGA CGCAGGTGAA
    GGAGCGCTAC AGCATGCAAG TCCGCAATGG CATCTCCCCC ACCAACCCCA CCAAGCTTTC
    CATCACGGAG AATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa152371
    Clone ID Related Accession (Same CDS sequence) XM_015290519.2
    Accession Version XM_015290519.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3429bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product ras GTPase-activating protein nGAP isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006095.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015290519.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCAGCTCG CAGAGGTCCC GGCAGAGAGA TCTCCTCGCA GACGGAGCAT CTCTGGGACC 
    AGCACATCTG AGAAAACCAA CTCCATGGAT GCTGCAAATA CCTCTCCTTT CAAAGTATCG
    GGCTTCTTCA GTAAACGTCT GAAAGGGTCC ATCAAGAGGA CAAAGAGTCA GTCAAAGCTG
    GACAGGAACA CAAGTTTCCG CCTCCCATCC CTCCGCAATG CTGATGACAG GTCACGTGGG
    CTGCCGAAGC TGAAGGAGTC CCGCTCTCAT GAGTCCCTGC TGAGCCCCGG CAGCGCAGTG
    GAAGCCCTGG ACCTTGGGGC AGAAGAAAAG GTCTTTGTAA AGCCACTGCA CAGCAGCATC
    CTCGGACAGG ACTTCTGCTT TGAGGTAACC TACTCCAGCG GCAGTAAATG CTTCAGCTGT
    TCCTCTGCTG CAGAGAGGGA TAAGTGGATG GAAAACCTAC GCAGAACAGT GCAGCCGAAT
    AAGGACAACT GCCGTCGAGC TGAAAATGTG CTCCGTCTCT GGATCATTGA GGCAAAGGAC
    CTGGCCCCCA AGAAGAAATA CTTCTGTGAG CTGTGCCTTG ATGACACTCT CTTTGCCCGC
    ACCACCAGCA AAACGAAAGC AGATAACATT TTCTGGGGGG AGCACTTTGA GTTCTACGGC
    CTCCCAGCTC TTCACAGCAT CACGGTTCAC ATCTACAAGG ATGTGGAGAA GAAAAAGAAA
    AAGGACAAAA ACAATTACGT CGGCCTGGTC AACATTCCCA TGGCCACTGT AACGGGGCGC
    CAGTTTGTGG AAAAATGGTA CCCAGTCAGC ACACCTACAC CCAACAAAGG GAAATCAGGA
    GGCCCTTCCA TTCGCATCAA GTCGCGTTAT CAGACCATCA CCATCTTACC CATGGAGCAA
    TACAAGGAGT TTGCAGAGTT TGTTACCAGC AACTACACGA TGCTGTGCTC TGTGCTGGAG
    CCAGTCATCA GCGTAAGGAA TAAGGAAGAG ATGGCTTGTG CAATGGTGCA CATTCTTCAG
    AGCACGGGGA GAGCCAAGGA CTTCCTAACG GATCTGGTGA TGTCTGAGGT GGATCGCTGC
    GGGGAACACG ATGTCTTGAT CTTCAGGGAG AACACACTTG CTACCAAAGC TATTGAGGAA
    TACCTGAAGT TGGTAGGGCA GAAGTATCTC CATGATGCGC TAGGGGAGTT TATCAAGGCT
    TTGTATGAGT CAGATGAAAA CTGTGAAGTG GACCCCAGCA AATGTTCATC CAGTGAATTA
    ACAGACCATC AGAGCAACCT GAAAATGTGT TGTGAGCTGG CCTTCTGCAA GATTATCAAT
    TCCTACTGCG TGTTCCCTCG TGAGCTGAAG GAGGTGTTTG CATCTTGGAA GCAGCAGTGC
    CTGAGCAGGG GCAAGCAGGA CATCAGCGAG CGGCTGATCA GTGCCTCCCT GTTCCTCCGC
    TTCCTGTGCC CCGCCATCAT GTCCCCCAGC CTCTTCAGCC TCATGCAGGA GTATCCTGAC
    GACCGGACAT CGCGCACGCT CACACTCATT GCTAAAGTCA TTCAGAATCT CGCCAATTTT
    GCTAAGTTTG GTAACAAGGA AGAGTACATG GCTTTCATGA ACGACTTTCT GGAACATGAG
    TGGGGGGGAA TGAAGCGATT TCTCCTGGAG ATCTCTAATC CAGATACCAT CTCAAACATG
    CCTGGATTTG AGGGCTATAT CGACCTGGGC AGAGAGCTCT CAGTTTTGCA TTCCCTCTTA
    TGGGAGGTTG TGTCCCAACT TGATAAGGGT GAAAATTCCT TCCTACAGGC GACCGTGGCA
    AAACTGGGAC CTCTTCCTCG TATTCTTGCC GACATCACCA AATCAATGAC CAACCCTACG
    CCAATACAGC AGCAGCTGAG GCGTTTCACT GAGCACAGCT CTAGTCCAAA CGTCAGTGGC
    AGTCTCTCCT CAGGGCTTCA GAAGATATTC GAGGACCCCA CTGATGGTGA CTTGCATAAA
    CTGAAGTCTC CAACCCAGGA AAACATAGAT GGCTATTTCA GGGGCAAGAC CTTGCTGTTG
    GTTCAGCAGG CGTCCACCCA GAGCATGACT TACTCAGATA AGGACGAAAG AGAGAGCATC
    TTGCCCAACG GACGTAGCAT CTCCCTCATG GACCTGCAGG ATCCCCATAC CGCTCACAGC
    GACCACGCTT CCATGATGCA CGACGTGCCT TTGCGCTTGG CCGGCAGCCA GCTCTCCATC
    ACCCAGGTGG CCAACATCAA GCAGCTGTGG GAGACCCAGA GCACGCCCCA AAGCGCTCCC
    CAGGTGAGGA GGCCGCTGCA CCCGGCTTTG AACCAGCAGG GCAGCCTCCA GCCTCTGTCA
    TTCCAGAACC CGGTGTACCA CCTCAACAAC CCACCTGCGC CGTTGCCCAA GGCCTCCGTG
    GACTCCAGCC TGGAGAACCT GAGCACCGCC AGCTCCCAGA GCCGAAGCAA CAGCGAAGAC
    TTCAAGCTCA GCGGGCCCAG CAACAGCAGC ATGGAGGACT TCACCAAGCG CAGCACGCAG
    AGCGAGGACT TCTCTAGGCG GCACACGGTC CCGGACAAGC ACGTCCCCAT CGCGTTGCCC
    CGCCAGAACA GCAGCAGCCA GGCACAGATC CGCAAAATGG ACCAGGCCGG GTTAGGGGCC
    AGGGCCAAAG CGCCGCAGTC GCTGCCGCAC AGCGCGTCCC TGCGCAGCAC GGGCAGCATG
    TCGGCCGTGT CGGGGGCCGT GGTGACTGAG CCCGTCCAGA ACGGCAGCCG CTCCCGGCAG
    CAGTCGTCCT CCTCCAGGGA GAGCCCTGTG CCGAAGGTGA GGGCCATTCA GAGGCAGCAA
    ACGCAGCAGG TCCAGTCTCC TGTGGACTCA GCCACAATGT CACCAGTGGA AAGGACAGCA
    GCGTGGGTTC TCAATAACGG GCAGTACGAG GAGGTCGAGG AGGACACAGA GCAGAACCCA
    GATGAAGCCA AGCATGCTGA GAAGTACGAA CAAGAGATAG CAAAGCTGAA GGAACGCTTG
    AAAGTGTCGA GCCGCCGGCT GGAGGAGTAC GAGCGGCGGC TCCTGGTGCA GGAGCAGCAA
    ATGCAGAAGC TGCTGATGGA GTACAAGTCC AGGCTGGAGG ACAGCGAGGA GAGACTGCGC
    AGGCAACAGG AGGAGAAGGA CAGCCAGATG AAAAGCATTA TCAGCAGGCT CATGGCAGTT
    GAAGAGGAAC TGAAGAAAGA TCATGCAGAG ATGCAAGCTG TCATCGATGC AAAGCAGAAA
    ATTATTGATG CACAGGTCAT TAATGGGAAT GAGATAATTC AAACAGGAGA AGCGGATCGT
    GTCCCTGGAC TCTGCCAACA CGCGGCTGAT GAGTGCCCTG ACGCAGGTGA AGGAGCGCTA
    CAGCATGCAA GTCCGCAATG GCATCTCCCC CACCAACCCC ACCAAGCTTT CCATCACGGA
    GAATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015146005.1
    CDS238..3666
    Translation

    Target ORF information:

    RefSeq Version XM_015290519.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015290519.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCAGCTCG CAGAGGTCCC GGCAGAGAGA TCTCCTCGCA GACGGAGCAT CTCTGGGACC 
    AGCACATCTG AGAAAACCAA CTCCATGGAT GCTGCAAATA CCTCTCCTTT CAAAGTATCG
    GGCTTCTTCA GTAAACGTCT GAAAGGGTCC ATCAAGAGGA CAAAGAGTCA GTCAAAGCTG
    GACAGGAACA CAAGTTTCCG CCTCCCATCC CTCCGCAATG CTGATGACAG GTCACGTGGG
    CTGCCGAAGC TGAAGGAGTC CCGCTCTCAT GAGTCCCTGC TGAGCCCCGG CAGCGCAGTG
    GAAGCCCTGG ACCTTGGGGC AGAAGAAAAG GTCTTTGTAA AGCCACTGCA CAGCAGCATC
    CTCGGACAGG ACTTCTGCTT TGAGGTAACC TACTCCAGCG GCAGTAAATG CTTCAGCTGT
    TCCTCTGCTG CAGAGAGGGA TAAGTGGATG GAAAACCTAC GCAGAACAGT GCAGCCGAAT
    AAGGACAACT GCCGTCGAGC TGAAAATGTG CTCCGTCTCT GGATCATTGA GGCAAAGGAC
    CTGGCCCCCA AGAAGAAATA CTTCTGTGAG CTGTGCCTTG ATGACACTCT CTTTGCCCGC
    ACCACCAGCA AAACGAAAGC AGATAACATT TTCTGGGGGG AGCACTTTGA GTTCTACGGC
    CTCCCAGCTC TTCACAGCAT CACGGTTCAC ATCTACAAGG ATGTGGAGAA GAAAAAGAAA
    AAGGACAAAA ACAATTACGT CGGCCTGGTC AACATTCCCA TGGCCACTGT AACGGGGCGC
    CAGTTTGTGG AAAAATGGTA CCCAGTCAGC ACACCTACAC CCAACAAAGG GAAATCAGGA
    GGCCCTTCCA TTCGCATCAA GTCGCGTTAT CAGACCATCA CCATCTTACC CATGGAGCAA
    TACAAGGAGT TTGCAGAGTT TGTTACCAGC AACTACACGA TGCTGTGCTC TGTGCTGGAG
    CCAGTCATCA GCGTAAGGAA TAAGGAAGAG ATGGCTTGTG CAATGGTGCA CATTCTTCAG
    AGCACGGGGA GAGCCAAGGA CTTCCTAACG GATCTGGTGA TGTCTGAGGT GGATCGCTGC
    GGGGAACACG ATGTCTTGAT CTTCAGGGAG AACACACTTG CTACCAAAGC TATTGAGGAA
    TACCTGAAGT TGGTAGGGCA GAAGTATCTC CATGATGCGC TAGGGGAGTT TATCAAGGCT
    TTGTATGAGT CAGATGAAAA CTGTGAAGTG GACCCCAGCA AATGTTCATC CAGTGAATTA
    ACAGACCATC AGAGCAACCT GAAAATGTGT TGTGAGCTGG CCTTCTGCAA GATTATCAAT
    TCCTACTGCG TGTTCCCTCG TGAGCTGAAG GAGGTGTTTG CATCTTGGAA GCAGCAGTGC
    CTGAGCAGGG GCAAGCAGGA CATCAGCGAG CGGCTGATCA GTGCCTCCCT GTTCCTCCGC
    TTCCTGTGCC CCGCCATCAT GTCCCCCAGC CTCTTCAGCC TCATGCAGGA GTATCCTGAC
    GACCGGACAT CGCGCACGCT CACACTCATT GCTAAAGTCA TTCAGAATCT CGCCAATTTT
    GCTAAGTTTG GTAACAAGGA AGAGTACATG GCTTTCATGA ACGACTTTCT GGAACATGAG
    TGGGGGGGAA TGAAGCGATT TCTCCTGGAG ATCTCTAATC CAGATACCAT CTCAAACATG
    CCTGGATTTG AGGGCTATAT CGACCTGGGC AGAGAGCTCT CAGTTTTGCA TTCCCTCTTA
    TGGGAGGTTG TGTCCCAACT TGATAAGGGT GAAAATTCCT TCCTACAGGC GACCGTGGCA
    AAACTGGGAC CTCTTCCTCG TATTCTTGCC GACATCACCA AATCAATGAC CAACCCTACG
    CCAATACAGC AGCAGCTGAG GCGTTTCACT GAGCACAGCT CTAGTCCAAA CGTCAGTGGC
    AGTCTCTCCT CAGGGCTTCA GAAGATATTC GAGGACCCCA CTGATGGTGA CTTGCATAAA
    CTGAAGTCTC CAACCCAGGA AAACATAGAT GGCTATTTCA GGGGCAAGAC CTTGCTGTTG
    GTTCAGCAGG CGTCCACCCA GAGCATGACT TACTCAGATA AGGACGAAAG AGAGAGCATC
    TTGCCCAACG GACGTAGCAT CTCCCTCATG GACCTGCAGG ATCCCCATAC CGCTCACAGC
    GACCACGCTT CCATGATGCA CGACGTGCCT TTGCGCTTGG CCGGCAGCCA GCTCTCCATC
    ACCCAGGTGG CCAACATCAA GCAGCTGTGG GAGACCCAGA GCACGCCCCA AAGCGCTCCC
    CAGGTGAGGA GGCCGCTGCA CCCGGCTTTG AACCAGCAGG GCAGCCTCCA GCCTCTGTCA
    TTCCAGAACC CGGTGTACCA CCTCAACAAC CCACCTGCGC CGTTGCCCAA GGCCTCCGTG
    GACTCCAGCC TGGAGAACCT GAGCACCGCC AGCTCCCAGA GCCGAAGCAA CAGCGAAGAC
    TTCAAGCTCA GCGGGCCCAG CAACAGCAGC ATGGAGGACT TCACCAAGCG CAGCACGCAG
    AGCGAGGACT TCTCTAGGCG GCACACGGTC CCGGACAAGC ACGTCCCCAT CGCGTTGCCC
    CGCCAGAACA GCAGCAGCCA GGCACAGATC CGCAAAATGG ACCAGGCCGG GTTAGGGGCC
    AGGGCCAAAG CGCCGCAGTC GCTGCCGCAC AGCGCGTCCC TGCGCAGCAC GGGCAGCATG
    TCGGCCGTGT CGGGGGCCGT GGTGACTGAG CCCGTCCAGA ACGGCAGCCG CTCCCGGCAG
    CAGTCGTCCT CCTCCAGGGA GAGCCCTGTG CCGAAGGTGA GGGCCATTCA GAGGCAGCAA
    ACGCAGCAGG TCCAGTCTCC TGTGGACTCA GCCACAATGT CACCAGTGGA AAGGACAGCA
    GCGTGGGTTC TCAATAACGG GCAGTACGAG GAGGTCGAGG AGGACACAGA GCAGAACCCA
    GATGAAGCCA AGCATGCTGA GAAGTACGAA CAAGAGATAG CAAAGCTGAA GGAACGCTTG
    AAAGTGTCGA GCCGCCGGCT GGAGGAGTAC GAGCGGCGGC TCCTGGTGCA GGAGCAGCAA
    ATGCAGAAGC TGCTGATGGA GTACAAGTCC AGGCTGGAGG ACAGCGAGGA GAGACTGCGC
    AGGCAACAGG AGGAGAAGGA CAGCCAGATG AAAAGCATTA TCAGCAGGCT CATGGCAGTT
    GAAGAGGAAC TGAAGAAAGA TCATGCAGAG ATGCAAGCTG TCATCGATGC AAAGCAGAAA
    ATTATTGATG CACAGGTCAT TAATGGGAAT GAGATAATTC AAACAGGAGA AGCGGATCGT
    GTCCCTGGAC TCTGCCAACA CGCGGCTGAT GAGTGCCCTG ACGCAGGTGA AGGAGCGCTA
    CAGCATGCAA GTCCGCAATG GCATCTCCCC CACCAACCCC ACCAAGCTTT CCATCACGGA
    GAATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa152372
    Clone ID Related Accession (Same CDS sequence) XM_015290520.2
    Accession Version XM_015290520.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3327bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product ras GTPase-activating protein nGAP isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006095.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015290520.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGGGCCGC CAAGCTGGGG CTTCTTCAGT AAACGTCTGA AAGGGTCCAT CAAGAGGACA 
    AAGAGTCAGT CAAAGCTGGA CAGGAACACA AGTTTCCGCC TCCCATCCCT CCGCAATGCT
    GATGACAGGT CACGTGGGCT GCCGAAGCTG AAGGAGTCCC GCTCTCATGA GTCCCTGCTG
    AGCCCCGGCA GCGCAGTGGA AGCCCTGGAC CTTGGGGCAG AAGAAAAGGT CTTTGTAAAG
    CCACTGCACA GCAGCATCCT CGGACAGGAC TTCTGCTTTG AGGTAACCTA CTCCAGCGGC
    AGTAAATGCT TCAGCTGTTC CTCTGCTGCA GAGAGGGATA AGTGGATGGA AAACCTACGC
    AGAACAGTGC AGCCGAATAA GGACAACTGC CGTCGAGCTG AAAATGTGCT CCGTCTCTGG
    ATCATTGAGG CAAAGGACCT GGCCCCCAAG AAGAAATACT TCTGTGAGCT GTGCCTTGAT
    GACACTCTCT TTGCCCGCAC CACCAGCAAA ACGAAAGCAG ATAACATTTT CTGGGGGGAG
    CACTTTGAGT TCTACGGCCT CCCAGCTCTT CACAGCATCA CGGTTCACAT CTACAAGGAT
    GTGGAGAAGA AAAAGAAAAA GGACAAAAAC AATTACGTCG GCCTGGTCAA CATTCCCATG
    GCCACTGTAA CGGGGCGCCA GTTTGTGGAA AAATGGTACC CAGTCAGCAC ACCTACACCC
    AACAAAGGGA AATCAGGAGG CCCTTCCATT CGCATCAAGT CGCGTTATCA GACCATCACC
    ATCTTACCCA TGGAGCAATA CAAGGAGTTT GCAGAGTTTG TTACCAGCAA CTACACGATG
    CTGTGCTCTG TGCTGGAGCC AGTCATCAGC GTAAGGAATA AGGAAGAGAT GGCTTGTGCA
    ATGGTGCACA TTCTTCAGAG CACGGGGAGA GCCAAGGACT TCCTAACGGA TCTGGTGATG
    TCTGAGGTGG ATCGCTGCGG GGAACACGAT GTCTTGATCT TCAGGGAGAA CACACTTGCT
    ACCAAAGCTA TTGAGGAATA CCTGAAGTTG GTAGGGCAGA AGTATCTCCA TGATGCGCTA
    GGGGAGTTTA TCAAGGCTTT GTATGAGTCA GATGAAAACT GTGAAGTGGA CCCCAGCAAA
    TGTTCATCCA GTGAATTAAC AGACCATCAG AGCAACCTGA AAATGTGTTG TGAGCTGGCC
    TTCTGCAAGA TTATCAATTC CTACTGCGTG TTCCCTCGTG AGCTGAAGGA GGTGTTTGCA
    TCTTGGAAGC AGCAGTGCCT GAGCAGGGGC AAGCAGGACA TCAGCGAGCG GCTGATCAGT
    GCCTCCCTGT TCCTCCGCTT CCTGTGCCCC GCCATCATGT CCCCCAGCCT CTTCAGCCTC
    ATGCAGGAGT ATCCTGACGA CCGGACATCG CGCACGCTCA CACTCATTGC TAAAGTCATT
    CAGAATCTCG CCAATTTTGC TAAGTTTGGT AACAAGGAAG AGTACATGGC TTTCATGAAC
    GACTTTCTGG AACATGAGTG GGGGGGAATG AAGCGATTTC TCCTGGAGAT CTCTAATCCA
    GATACCATCT CAAACATGCC TGGATTTGAG GGCTATATCG ACCTGGGCAG AGAGCTCTCA
    GTTTTGCATT CCCTCTTATG GGAGGTTGTG TCCCAACTTG ATAAGGGTGA AAATTCCTTC
    CTACAGGCGA CCGTGGCAAA ACTGGGACCT CTTCCTCGTA TTCTTGCCGA CATCACCAAA
    TCAATGACCA ACCCTACGCC AATACAGCAG CAGCTGAGGC GTTTCACTGA GCACAGCTCT
    AGTCCAAACG TCAGTGGCAG TCTCTCCTCA GGGCTTCAGA AGATATTCGA GGACCCCACT
    GATGGTGACT TGCATAAACT GAAGTCTCCA ACCCAGGAAA ACATAGATGG CTATTTCAGG
    GGCAAGACCT TGCTGTTGGT TCAGCAGGCG TCCACCCAGA GCATGACTTA CTCAGATAAG
    GACGAAAGAG AGAGCATCTT GCCCAACGGA CGTAGCATCT CCCTCATGGA CCTGCAGGAT
    CCCCATACCG CTCACAGCGA CCACGCTTCC ATGATGCACG ACGTGCCTTT GCGCTTGGCC
    GGCAGCCAGC TCTCCATCAC CCAGGTGGCC AACATCAAGC AGCTGTGGGA GACCCAGAGC
    ACGCCCCAAA GCGCTCCCCA GGTGAGGAGG CCGCTGCACC CGGCTTTGAA CCAGCAGGGC
    AGCCTCCAGC CTCTGTCATT CCAGAACCCG GTGTACCACC TCAACAACCC ACCTGCGCCG
    TTGCCCAAGG CCTCCGTGGA CTCCAGCCTG GAGAACCTGA GCACCGCCAG CTCCCAGAGC
    CGAAGCAACA GCGAAGACTT CAAGCTCAGC GGGCCCAGCA ACAGCAGCAT GGAGGACTTC
    ACCAAGCGCA GCACGCAGAG CGAGGACTTC TCTAGGCGGC ACACGGTCCC GGACAAGCAC
    GTCCCCATCG CGTTGCCCCG CCAGAACAGC AGCAGCCAGG CACAGATCCG CAAAATGGAC
    CAGGCCGGGT TAGGGGCCAG GGCCAAAGCG CCGCAGTCGC TGCCGCACAG CGCGTCCCTG
    CGCAGCACGG GCAGCATGTC GGCCGTGTCG GGGGCCGTGG TGACTGAGCC CGTCCAGAAC
    GGCAGCCGCT CCCGGCAGCA GTCGTCCTCC TCCAGGGAGA GCCCTGTGCC GAAGGTGAGG
    GCCATTCAGA GGCAGCAAAC GCAGCAGGTC CAGTCTCCTG TGGACTCAGC CACAATGTCA
    CCAGTGGAAA GGACAGCAGC GTGGGTTCTC AATAACGGGC AGTACGAGGA GGTCGAGGAG
    GACACAGAGC AGAACCCAGA TGAAGCCAAG CATGCTGAGA AGTACGAACA AGAGATAGCA
    AAGCTGAAGG AACGCTTGAA AGTGTCGAGC CGCCGGCTGG AGGAGTACGA GCGGCGGCTC
    CTGGTGCAGG AGCAGCAAAT GCAGAAGCTG CTGATGGAGT ACAAGTCCAG GCTGGAGGAC
    AGCGAGGAGA GACTGCGCAG GCAACAGGAG GAGAAGGACA GCCAGATGAA AAGCATTATC
    AGCAGGCTCA TGGCAGTTGA AGAGGAACTG AAGAAAGATC ATGCAGAGAT GCAAGCTGTC
    ATCGATGCAA AGCAGAAAAT TATTGATGCA CAGGTCATTA ATGGGAATGA GATAATTCAA
    ACAGGAGAAG CGGATCGTGT CCCTGGACTC TGCCAACACG CGGCTGATGA GTGCCCTGAC
    GCAGGTGAAG GAGCGCTACA GCATGCAAGT CCGCAATGGC ATCTCCCCCA CCAACCCCAC
    CAAGCTTTCC ATCACGGAGA ATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015146006.1
    CDS253..3579
    Translation

    Target ORF information:

    RefSeq Version XM_015290520.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015290520.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGGGCCGC CAAGCTGGGG CTTCTTCAGT AAACGTCTGA AAGGGTCCAT CAAGAGGACA 
    AAGAGTCAGT CAAAGCTGGA CAGGAACACA AGTTTCCGCC TCCCATCCCT CCGCAATGCT
    GATGACAGGT CACGTGGGCT GCCGAAGCTG AAGGAGTCCC GCTCTCATGA GTCCCTGCTG
    AGCCCCGGCA GCGCAGTGGA AGCCCTGGAC CTTGGGGCAG AAGAAAAGGT CTTTGTAAAG
    CCACTGCACA GCAGCATCCT CGGACAGGAC TTCTGCTTTG AGGTAACCTA CTCCAGCGGC
    AGTAAATGCT TCAGCTGTTC CTCTGCTGCA GAGAGGGATA AGTGGATGGA AAACCTACGC
    AGAACAGTGC AGCCGAATAA GGACAACTGC CGTCGAGCTG AAAATGTGCT CCGTCTCTGG
    ATCATTGAGG CAAAGGACCT GGCCCCCAAG AAGAAATACT TCTGTGAGCT GTGCCTTGAT
    GACACTCTCT TTGCCCGCAC CACCAGCAAA ACGAAAGCAG ATAACATTTT CTGGGGGGAG
    CACTTTGAGT TCTACGGCCT CCCAGCTCTT CACAGCATCA CGGTTCACAT CTACAAGGAT
    GTGGAGAAGA AAAAGAAAAA GGACAAAAAC AATTACGTCG GCCTGGTCAA CATTCCCATG
    GCCACTGTAA CGGGGCGCCA GTTTGTGGAA AAATGGTACC CAGTCAGCAC ACCTACACCC
    AACAAAGGGA AATCAGGAGG CCCTTCCATT CGCATCAAGT CGCGTTATCA GACCATCACC
    ATCTTACCCA TGGAGCAATA CAAGGAGTTT GCAGAGTTTG TTACCAGCAA CTACACGATG
    CTGTGCTCTG TGCTGGAGCC AGTCATCAGC GTAAGGAATA AGGAAGAGAT GGCTTGTGCA
    ATGGTGCACA TTCTTCAGAG CACGGGGAGA GCCAAGGACT TCCTAACGGA TCTGGTGATG
    TCTGAGGTGG ATCGCTGCGG GGAACACGAT GTCTTGATCT TCAGGGAGAA CACACTTGCT
    ACCAAAGCTA TTGAGGAATA CCTGAAGTTG GTAGGGCAGA AGTATCTCCA TGATGCGCTA
    GGGGAGTTTA TCAAGGCTTT GTATGAGTCA GATGAAAACT GTGAAGTGGA CCCCAGCAAA
    TGTTCATCCA GTGAATTAAC AGACCATCAG AGCAACCTGA AAATGTGTTG TGAGCTGGCC
    TTCTGCAAGA TTATCAATTC CTACTGCGTG TTCCCTCGTG AGCTGAAGGA GGTGTTTGCA
    TCTTGGAAGC AGCAGTGCCT GAGCAGGGGC AAGCAGGACA TCAGCGAGCG GCTGATCAGT
    GCCTCCCTGT TCCTCCGCTT CCTGTGCCCC GCCATCATGT CCCCCAGCCT CTTCAGCCTC
    ATGCAGGAGT ATCCTGACGA CCGGACATCG CGCACGCTCA CACTCATTGC TAAAGTCATT
    CAGAATCTCG CCAATTTTGC TAAGTTTGGT AACAAGGAAG AGTACATGGC TTTCATGAAC
    GACTTTCTGG AACATGAGTG GGGGGGAATG AAGCGATTTC TCCTGGAGAT CTCTAATCCA
    GATACCATCT CAAACATGCC TGGATTTGAG GGCTATATCG ACCTGGGCAG AGAGCTCTCA
    GTTTTGCATT CCCTCTTATG GGAGGTTGTG TCCCAACTTG ATAAGGGTGA AAATTCCTTC
    CTACAGGCGA CCGTGGCAAA ACTGGGACCT CTTCCTCGTA TTCTTGCCGA CATCACCAAA
    TCAATGACCA ACCCTACGCC AATACAGCAG CAGCTGAGGC GTTTCACTGA GCACAGCTCT
    AGTCCAAACG TCAGTGGCAG TCTCTCCTCA GGGCTTCAGA AGATATTCGA GGACCCCACT
    GATGGTGACT TGCATAAACT GAAGTCTCCA ACCCAGGAAA ACATAGATGG CTATTTCAGG
    GGCAAGACCT TGCTGTTGGT TCAGCAGGCG TCCACCCAGA GCATGACTTA CTCAGATAAG
    GACGAAAGAG AGAGCATCTT GCCCAACGGA CGTAGCATCT CCCTCATGGA CCTGCAGGAT
    CCCCATACCG CTCACAGCGA CCACGCTTCC ATGATGCACG ACGTGCCTTT GCGCTTGGCC
    GGCAGCCAGC TCTCCATCAC CCAGGTGGCC AACATCAAGC AGCTGTGGGA GACCCAGAGC
    ACGCCCCAAA GCGCTCCCCA GGTGAGGAGG CCGCTGCACC CGGCTTTGAA CCAGCAGGGC
    AGCCTCCAGC CTCTGTCATT CCAGAACCCG GTGTACCACC TCAACAACCC ACCTGCGCCG
    TTGCCCAAGG CCTCCGTGGA CTCCAGCCTG GAGAACCTGA GCACCGCCAG CTCCCAGAGC
    CGAAGCAACA GCGAAGACTT CAAGCTCAGC GGGCCCAGCA ACAGCAGCAT GGAGGACTTC
    ACCAAGCGCA GCACGCAGAG CGAGGACTTC TCTAGGCGGC ACACGGTCCC GGACAAGCAC
    GTCCCCATCG CGTTGCCCCG CCAGAACAGC AGCAGCCAGG CACAGATCCG CAAAATGGAC
    CAGGCCGGGT TAGGGGCCAG GGCCAAAGCG CCGCAGTCGC TGCCGCACAG CGCGTCCCTG
    CGCAGCACGG GCAGCATGTC GGCCGTGTCG GGGGCCGTGG TGACTGAGCC CGTCCAGAAC
    GGCAGCCGCT CCCGGCAGCA GTCGTCCTCC TCCAGGGAGA GCCCTGTGCC GAAGGTGAGG
    GCCATTCAGA GGCAGCAAAC GCAGCAGGTC CAGTCTCCTG TGGACTCAGC CACAATGTCA
    CCAGTGGAAA GGACAGCAGC GTGGGTTCTC AATAACGGGC AGTACGAGGA GGTCGAGGAG
    GACACAGAGC AGAACCCAGA TGAAGCCAAG CATGCTGAGA AGTACGAACA AGAGATAGCA
    AAGCTGAAGG AACGCTTGAA AGTGTCGAGC CGCCGGCTGG AGGAGTACGA GCGGCGGCTC
    CTGGTGCAGG AGCAGCAAAT GCAGAAGCTG CTGATGGAGT ACAAGTCCAG GCTGGAGGAC
    AGCGAGGAGA GACTGCGCAG GCAACAGGAG GAGAAGGACA GCCAGATGAA AAGCATTATC
    AGCAGGCTCA TGGCAGTTGA AGAGGAACTG AAGAAAGATC ATGCAGAGAT GCAAGCTGTC
    ATCGATGCAA AGCAGAAAAT TATTGATGCA CAGGTCATTA ATGGGAATGA GATAATTCAA
    ACAGGAGAAG CGGATCGTGT CCCTGGACTC TGCCAACACG CGGCTGATGA GTGCCCTGAC
    GCAGGTGAAG GAGCGCTACA GCATGCAAGT CCGCAATGGC ATCTCCCCCA CCAACCCCAC
    CAAGCTTTCC ATCACGGAGA ATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39039
    Clone ID Related Accession (Same CDS sequence) XM_015290510.1
    Accession Version XM_015290510.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4296bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product ras GTPase-activating protein nGAP isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAGTGCTT TTAAAAAGTA TTACAGTCTC TCTCCCAGGA CCTTTAGGAG GTCTTTGTCG 
    GAGCAAGATG CTGGCACCGC TGCCTTAAAG GACTATAATT GGTACCACCA AATGAGAAGC
    AAGAAGGGGA GCAAACAAAC AGCTTTGGAC CGAGAGAAAG CACTCGGAGC GTACAGGTGG
    CAAACACATC CCCGTCTTCC CTGGAGGACA AGCAGAAGAG CACACTCTTG CAGCCTCAGA
    GGGGAAGGTA ATGATTCGAG TCTTGTCAAA AGTTCTGCAA GCATGGATGC TACCAGCGCC
    GACGATCTCC ACCAAGACTC AACGGAGATG CTCTTCCAGG CAAGGACCAG ATCACCGCGG
    GTTCCCTTCA ACCGCTCCCT GTCTGAACCT GTGCCGCACT CTGCAAACAG GAATGCCAAG
    CCCTTTCTGC AGGCGGTGCG CTCTGTGGAT TGTGAGCAGC ACAGCTATTT TGTCCGTGGC
    ATCTTCTCAA CTCTCTCAAG TGGCTTTTCA AAGATAATAA ATCGAAGGGG TGAGCAGCCC
    CCCACTGAGC CACTGGAGGA GTGGAAACCA GCCGGTAGTC AAATTGACCC GTGCCCAGGT
    AATGGACTCT GTTCTGTTTG TGTTACAGAT GTAAAAGGAC CACCTACGCA CAGGCTGTCC
    TGCGGCCAGT CCCCCTACAC CGAGACGACA ACATGGGAGA GGAAGTACTG CATCCTGACT
    GACAGCCAGC TGGTGCTGCT CAACAGGGAG AAGGAGGTGC CCACCGAGGG GTTGCAGGAG
    CCGCAGGATG CAACCAAAGG CCGCTGTCTG CGCCGCACGG TGAGCGTGCC CTCCGAGGGC
    CAGTTCCCCG AGTACCCACC AGATGGGGCC GCCAAGCTGG AGGTCCCGGC AGAGAGATCT
    CCTCGCAGAC GGAGCATCTC TGGGACCAGC ACATCTGAGA AAACCAACTC CATGGATGCT
    GCAAATACCT CTCCTTTCAA AGTATCGGGC TTCTTCAGTA AACGTCTGAA AGGGTCCATC
    AAGAGGACAA AGAGTCAGTC AAAGCTGGAC AGGAACACAA GTTTCCGCCT CCCATCCCTC
    CGCAATGCTG ATGACAGGTC ACGTGGGCTG CCGAAGCTGA AGGAGTCCCG CTCTCATGAG
    TCCCTGCTGA GCCCCGGCAG CGCAGTGGAA GCCCTGGACC TTGGGGCAGA AGAAAAGGTC
    TTTGTAAAGC CACTGCACAG CAGCATCCTC GGACAGGACT TCTGCTTTGA GGTAACCTAC
    TCCAGCGGCA GTAAATGCTT CAGCTGTTCC TCTGCTGCAG AGAGGGATAA GTGGATGGAA
    AACCTACGCA GAACAGTGCA GCCGAATAAG GACAACTGCC GTCGAGCTGA AAATGTGCTC
    CGTCTCTGGA TCATTGAGGC AAAGGACCTG GCCCCCAAGA AGAAATACTT CTGTGAGCTG
    TGCCTTGATG ACACTCTCTT TGCCCGCACC ACCAGCAAAA CGAAAGCAGA TAACATTTTC
    TGGGGGGAGC ACTTTGAGTT CTACGGCCTC CCAGCTCTTC ACAGCATCAC GGTTCACATC
    TACAAGGATG TGGAGAAGAA AAAGAAAAAG GACAAAAACA ATTACGTCGG CCTGGTCAAC
    ATTCCCATGG CCACTGTAAC GGGGCGCCAG TTTGTGGAAA AATGGTACCC AGTCAGCACA
    CCTACACCCA ACAAAGGGAA ATCAGGAGGC CCTTCCATTC GCATCAAGTC GCGTTATCAG
    ACCATCACCA TCTTACCCAT GGAGCAATAC AAGGAGTTTG CAGAGTTTGT TACCAGCAAC
    TACACGATGC TGTGCTCTGT GCTGGAGCCA GTCATCAGCG TAAGGAATAA GGAAGAGATG
    GCTTGTGCAA TGGTGCACAT TCTTCAGAGC ACGGGGAGAG CCAAGGACTT CCTAACGGAT
    CTGGTGATGT CTGAGGTGGA TCGCTGCGGG GAACACGATG TCTTGATCTT CAGGGAGAAC
    ACACTTGCTA CCAAAGCTAT TGAGGAATAC CTGAAGTTGG TAGGGCAGAA GTATCTCCAT
    GATGCGCTAG GGGAGTTTAT CAAGGCTTTG TATGAGTCAG ATGAAAACTG TGAAGTGGAC
    CCCAGCAAAT GTTCATCCAG TGAATTAACA GACCATCAGA GCAACCTGAA AATGTGTTGT
    GAGCTGGCCT TCTGCAAGAT TATCAATTCC TACTGCGTGT TCCCTCGTGA GCTGAAGGAG
    GTGTTTGCAT CTTGGAAGCA GCAGTGCCTG AGCAGGGGCA AGCAGGACAT CAGCGAGCGG
    CTGATCAGTG CCTCCCTGTT CCTCCGCTTC CTCTGCCCCG CCATCATGTC CCCCAGCCTC
    TTCAGCCTCA TGCAGGAGTA TCCTGACGAC CGGACATCGC GCACGCTCAC ACTCATTGCT
    AAAGTCATTC AGAATCTCGC CAATTTTGCT AAGTTTGGTA ACAAGGAAGA GTACATGGCT
    TTCATGAACG ACTTTCTGGA ACATGAGTGG GGGGGAATGA AGCGATTTCT CCTGGAGATC
    TCTAATCCAG ATACCATCTC AAACATGCCT GGATTTGAGG GCTATATCGA CCTGGGCAGA
    GAGCTCTCAG TTTTGCATTC CCTCTTATGG GAGGTTGTGT CCCAACTTGA TAAGGGTGAA
    AATTCCTTCC TACAGGCGAC CGTGGCAAAA CTGGGACCTC TTCCTCGTAT TCTTGCCGAC
    ATCACCAAAT CAATGACCAA CCCTACGCCA ATACAGCAGC AGCTGAGGCG TTTCACTGAG
    CACAGCTCTA GTCCAAACGT CAGTGGCAGT CTCTCCTCAG GGCTTCAGAA GATATTCGAG
    GACCCCACTG ATGGTGACTT GCATAAACTG AAGTCTCCAA CCCAGGAAAA CATAGATGGC
    TATTTCAGGG GCAAGACCTT GCTGTTGGTT CAGCAGGCGT CCACCCAGAG CATGACTTAC
    TCAGATAAGG ACGAAAGAGA GAGCATCTTG CCCAACGGAC GTAGCATCTC CCTCATGGAC
    CTGCAGGATC CCCATACCGC TCACAGCGAC CACGCTTCCA TGATGCACGA CGTGCCTTTG
    CGCTTGGCCG GCAGCCAGCT CTCCATCACC CAGGTGGCCA ACATCAAGCA GCTGTGGGAG
    ACCCAGAGCA CGCCCCAAAG CGCTCCCCAG GTGAGGAGGC CGCTGCACCC GGCTTTGAAC
    CAGCAGGGCA GCCTCCAGCC TCTGTCATTC CAGAACCCGG TGTACCACCT CAACAACCCA
    CCTGCGCCGT TGCCCAAGGC CTCCGTGGAC TCCAGCCTGG AGAACCTGAG CACCGCCAGC
    TCCCAGAGCC GAAGCAACAG CGAAGACTTC AAGCTCAGCG GGCCCAGCAA CAGCAGCATG
    GAGGACTTCA CCAAGCGCAG CACGCAGAGC GAGGACTTCT CTAGGCGGCA CACGGTCCCG
    GACAAGCACG TCCCCATCGC GTTGCCCCGC CAGAACAGCA GCAGCCAGGC ACAGATCCGC
    AAAATGGACC AGGCCGGGTT AGGGGCCAGG GCCAAAGCGC CGCAGTCGCT GCCGCACAGC
    GCGTCCCTGC GCAGCACGGG CAGCATGTCG GCCGTGTCGG GGGCCGTGGT GACTGAGCCC
    GTCCAGAACG GCAGCCGCTC CCGGCAGCAG TCGTCCTCCT CCAGGGAGAG CCCTGTGCCG
    AAGGTGAGGG CCATTCAGAG GCAGCAAACG CAGCAGGTCC AGTCTCCTGT GGACTCAGCC
    ACAATGTCAC CAGTGGAAAG GACAGCAGCG TGGGTTCTCA ATAACGGGCA GTACGAGGAG
    GTCGAGGAGG ACACAGAGCA GAACCCAGAT GAAGCCAAGC ATGCTGAGAA GTACGAACAA
    GAGATAGCAA AGCTGAAGGA ACGCTTGAAA GTGTCGAGCC GCCGGCTGGA GGAGTACGAG
    CGGCGGCTCC TGGTGCAGGA GCAGCAAATG CAGAAGCTGC TGATGGAGTA CAAGTCCAGG
    CTGGAGGACA GCGAGGAGAG ACTGCGCAGG CAACAGGAGG AGAAGGACAG CCAGATGAAA
    AGCATTATCA GCAGGCTCAT GGCAGTTGAA GAGGAACTGA AGAAAGATCA TGCAGAGATG
    CAAGCTGTCA TCGATGCAAA GCAGAAAATT ATTGATGCAC AGGTCATTAA TGGGAATGAG
    ATAATTCAAA CAGGAGAAGC GGATCGTGTC CCTGGACTCT GCCAACACGC GGCTGATGAG
    TGCCCTGACG CAGGTGAAGG AGCGCTACAG CATGCAAGTC CGCAATGGCA TCTCCCCCAC
    CAACCCCACC AAGCTTTCCA TCACGGAGAA TGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015145996.1
    CDS638..4933
    Translation

    Target ORF information:

    RefSeq Version XM_015290510.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015290510.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAGTGCTT TTAAAAAGTA TTACAGTCTC TCTCCCAGGA CCTTTAGGAG GTCTTTGTCG 
    GAGCAAGATG CTGGCACCGC TGCCTTAAAG GACTATAATT GGTACCACCA AATGAGAAGC
    AAGAAGGGGA GCAAACAAAC AGCTTTGGAC CGAGAGAAAG CACTCGGAGC GTACAGGTGG
    CAAACACATC CCCGTCTTCC CTGGAGGACA AGCAGAAGAG CACACTCTTG CAGCCTCAGA
    GGGGAAGGTA ATGATTCGAG TCTTGTCAAA AGTTCTGCAA GCATGGATGC TACCAGCGCC
    GACGATCTCC ACCAAGACTC AACGGAGATG CTCTTCCAGG CAAGGACCAG ATCACCGCGG
    GTTCCCTTCA ACCGCTCCCT GTCTGAACCT GTGCCGCACT CTGCAAACAG GAATGCCAAG
    CCCTTTCTGC AGGCGGTGCG CTCTGTGGAT TGTGAGCAGC ACAGCTATTT TGTCCGTGGC
    ATCTTCTCAA CTCTCTCAAG TGGCTTTTCA AAGATAATAA ATCGAAGGGG TGAGCAGCCC
    CCCACTGAGC CACTGGAGGA GTGGAAACCA GCCGGTAGTC AAATTGACCC GTGCCCAGGT
    AATGGACTCT GTTCTGTTTG TGTTACAGAT GTAAAAGGAC CACCTACGCA CAGGCTGTCC
    TGCGGCCAGT CCCCCTACAC CGAGACGACA ACATGGGAGA GGAAGTACTG CATCCTGACT
    GACAGCCAGC TGGTGCTGCT CAACAGGGAG AAGGAGGTGC CCACCGAGGG GTTGCAGGAG
    CCGCAGGATG CAACCAAAGG CCGCTGTCTG CGCCGCACGG TGAGCGTGCC CTCCGAGGGC
    CAGTTCCCCG AGTACCCACC AGATGGGGCC GCCAAGCTGG AGGTCCCGGC AGAGAGATCT
    CCTCGCAGAC GGAGCATCTC TGGGACCAGC ACATCTGAGA AAACCAACTC CATGGATGCT
    GCAAATACCT CTCCTTTCAA AGTATCGGGC TTCTTCAGTA AACGTCTGAA AGGGTCCATC
    AAGAGGACAA AGAGTCAGTC AAAGCTGGAC AGGAACACAA GTTTCCGCCT CCCATCCCTC
    CGCAATGCTG ATGACAGGTC ACGTGGGCTG CCGAAGCTGA AGGAGTCCCG CTCTCATGAG
    TCCCTGCTGA GCCCCGGCAG CGCAGTGGAA GCCCTGGACC TTGGGGCAGA AGAAAAGGTC
    TTTGTAAAGC CACTGCACAG CAGCATCCTC GGACAGGACT TCTGCTTTGA GGTAACCTAC
    TCCAGCGGCA GTAAATGCTT CAGCTGTTCC TCTGCTGCAG AGAGGGATAA GTGGATGGAA
    AACCTACGCA GAACAGTGCA GCCGAATAAG GACAACTGCC GTCGAGCTGA AAATGTGCTC
    CGTCTCTGGA TCATTGAGGC AAAGGACCTG GCCCCCAAGA AGAAATACTT CTGTGAGCTG
    TGCCTTGATG ACACTCTCTT TGCCCGCACC ACCAGCAAAA CGAAAGCAGA TAACATTTTC
    TGGGGGGAGC ACTTTGAGTT CTACGGCCTC CCAGCTCTTC ACAGCATCAC GGTTCACATC
    TACAAGGATG TGGAGAAGAA AAAGAAAAAG GACAAAAACA ATTACGTCGG CCTGGTCAAC
    ATTCCCATGG CCACTGTAAC GGGGCGCCAG TTTGTGGAAA AATGGTACCC AGTCAGCACA
    CCTACACCCA ACAAAGGGAA ATCAGGAGGC CCTTCCATTC GCATCAAGTC GCGTTATCAG
    ACCATCACCA TCTTACCCAT GGAGCAATAC AAGGAGTTTG CAGAGTTTGT TACCAGCAAC
    TACACGATGC TGTGCTCTGT GCTGGAGCCA GTCATCAGCG TAAGGAATAA GGAAGAGATG
    GCTTGTGCAA TGGTGCACAT TCTTCAGAGC ACGGGGAGAG CCAAGGACTT CCTAACGGAT
    CTGGTGATGT CTGAGGTGGA TCGCTGCGGG GAACACGATG TCTTGATCTT CAGGGAGAAC
    ACACTTGCTA CCAAAGCTAT TGAGGAATAC CTGAAGTTGG TAGGGCAGAA GTATCTCCAT
    GATGCGCTAG GGGAGTTTAT CAAGGCTTTG TATGAGTCAG ATGAAAACTG TGAAGTGGAC
    CCCAGCAAAT GTTCATCCAG TGAATTAACA GACCATCAGA GCAACCTGAA AATGTGTTGT
    GAGCTGGCCT TCTGCAAGAT TATCAATTCC TACTGCGTGT TCCCTCGTGA GCTGAAGGAG
    GTGTTTGCAT CTTGGAAGCA GCAGTGCCTG AGCAGGGGCA AGCAGGACAT CAGCGAGCGG
    CTGATCAGTG CCTCCCTGTT CCTCCGCTTC CTCTGCCCCG CCATCATGTC CCCCAGCCTC
    TTCAGCCTCA TGCAGGAGTA TCCTGACGAC CGGACATCGC GCACGCTCAC ACTCATTGCT
    AAAGTCATTC AGAATCTCGC CAATTTTGCT AAGTTTGGTA ACAAGGAAGA GTACATGGCT
    TTCATGAACG ACTTTCTGGA ACATGAGTGG GGGGGAATGA AGCGATTTCT CCTGGAGATC
    TCTAATCCAG ATACCATCTC AAACATGCCT GGATTTGAGG GCTATATCGA CCTGGGCAGA
    GAGCTCTCAG TTTTGCATTC CCTCTTATGG GAGGTTGTGT CCCAACTTGA TAAGGGTGAA
    AATTCCTTCC TACAGGCGAC CGTGGCAAAA CTGGGACCTC TTCCTCGTAT TCTTGCCGAC
    ATCACCAAAT CAATGACCAA CCCTACGCCA ATACAGCAGC AGCTGAGGCG TTTCACTGAG
    CACAGCTCTA GTCCAAACGT CAGTGGCAGT CTCTCCTCAG GGCTTCAGAA GATATTCGAG
    GACCCCACTG ATGGTGACTT GCATAAACTG AAGTCTCCAA CCCAGGAAAA CATAGATGGC
    TATTTCAGGG GCAAGACCTT GCTGTTGGTT CAGCAGGCGT CCACCCAGAG CATGACTTAC
    TCAGATAAGG ACGAAAGAGA GAGCATCTTG CCCAACGGAC GTAGCATCTC CCTCATGGAC
    CTGCAGGATC CCCATACCGC TCACAGCGAC CACGCTTCCA TGATGCACGA CGTGCCTTTG
    CGCTTGGCCG GCAGCCAGCT CTCCATCACC CAGGTGGCCA ACATCAAGCA GCTGTGGGAG
    ACCCAGAGCA CGCCCCAAAG CGCTCCCCAG GTGAGGAGGC CGCTGCACCC GGCTTTGAAC
    CAGCAGGGCA GCCTCCAGCC TCTGTCATTC CAGAACCCGG TGTACCACCT CAACAACCCA
    CCTGCGCCGT TGCCCAAGGC CTCCGTGGAC TCCAGCCTGG AGAACCTGAG CACCGCCAGC
    TCCCAGAGCC GAAGCAACAG CGAAGACTTC AAGCTCAGCG GGCCCAGCAA CAGCAGCATG
    GAGGACTTCA CCAAGCGCAG CACGCAGAGC GAGGACTTCT CTAGGCGGCA CACGGTCCCG
    GACAAGCACG TCCCCATCGC GTTGCCCCGC CAGAACAGCA GCAGCCAGGC ACAGATCCGC
    AAAATGGACC AGGCCGGGTT AGGGGCCAGG GCCAAAGCGC CGCAGTCGCT GCCGCACAGC
    GCGTCCCTGC GCAGCACGGG CAGCATGTCG GCCGTGTCGG GGGCCGTGGT GACTGAGCCC
    GTCCAGAACG GCAGCCGCTC CCGGCAGCAG TCGTCCTCCT CCAGGGAGAG CCCTGTGCCG
    AAGGTGAGGG CCATTCAGAG GCAGCAAACG CAGCAGGTCC AGTCTCCTGT GGACTCAGCC
    ACAATGTCAC CAGTGGAAAG GACAGCAGCG TGGGTTCTCA ATAACGGGCA GTACGAGGAG
    GTCGAGGAGG ACACAGAGCA GAACCCAGAT GAAGCCAAGC ATGCTGAGAA GTACGAACAA
    GAGATAGCAA AGCTGAAGGA ACGCTTGAAA GTGTCGAGCC GCCGGCTGGA GGAGTACGAG
    CGGCGGCTCC TGGTGCAGGA GCAGCAAATG CAGAAGCTGC TGATGGAGTA CAAGTCCAGG
    CTGGAGGACA GCGAGGAGAG ACTGCGCAGG CAACAGGAGG AGAAGGACAG CCAGATGAAA
    AGCATTATCA GCAGGCTCAT GGCAGTTGAA GAGGAACTGA AGAAAGATCA TGCAGAGATG
    CAAGCTGTCA TCGATGCAAA GCAGAAAATT ATTGATGCAC AGGTCATTAA TGGGAATGAG
    ATAATTCAAA CAGGAGAAGC GGATCGTGTC CCTGGACTCT GCCAACACGC GGCTGATGAG
    TGCCCTGACG CAGGTGAAGG AGCGCTACAG CATGCAAGTC CGCAATGGCA TCTCCCCCAC
    CAACCCCACC AAGCTTTCCA TCACGGAGAA TGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39040
    Clone ID Related Accession (Same CDS sequence) XM_004943245.2
    Accession Version XM_004943245.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4287bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product ras GTPase-activating protein nGAP isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004943245.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAGTGCTT TTAAAAAGTA TTACAGTCTC TCTCCCAGGA CCTTTAGGAG GTCTTTGTCG 
    GAGCAAGATG CTGGCACCGC TGCCTTAAAG GACTATAATT GGTACCACCA AATGAGAAGC
    AAGAAGGGGA GCAAACAAAC AGCTTTGGAC CGAGAGAAAG CACTCGGAGC GTACAGGTGG
    CAAACACATC CCCGTCTTCC CTGGAGGACA AGCAGAAGAG CACACTCTTG CAGCCTCAGA
    GGGGAAGGTA ATGATTCGAG TCTTGTCAAA AGTTCTGCAA GCATGGATGC TACCAGCGCC
    GACGATCTCC ACCAAGACTC AACGGAGATG CTCTTCCAGG CAAGGACCAG ATCACCGCGG
    GTTCCCTTCA ACCGCTCCCT GTCTGAACCT GTGCCGCACT CTGCAAACAG GAATGCCAAG
    CCCTTTCTGC AGGCGGTGCG CTCTGTGGAT TGTGAGCAGC ACAGCTATTT TGTCCGTGGC
    ATCTTCTCAA CTCTCTCAAG TGGCTTTTCA AAGATAATAA ATCGAAGGGG TGAGCAGCCC
    CCCACTGAGC CACTGGAGGA GTGGAAACCA GCCGGTAGTC AAATTGACCC GTGCCCAGGT
    AATGGACTCT GTTCTGTTTG TGTTACAGAT GTAAAAGGAC CACCTACGCA CAGGCTGTCC
    TGCGGCCAGT CCCCCTACAC CGAGACGACA ACATGGGAGA GGAAGTACTG CATCCTGACT
    GACAGCCAGC TGGTGCTGCT CAACAGGGAG AAGGAGGTGC CCACCGAGGG GTTGCAGGAG
    CCGCAGGATG CAACCAAAGG CCGCTGTCTG CGCCGCACGG TGAGCGTGCC CTCCGAGGGC
    CAGTTCCCCG AGTACCCACC AGATGGGGCC GCCAAGCTGG AGGTCCCGGC AGAGAGATCT
    CCTCGCAGAC GGAGCATCTC TGGGACCAGC ACATCTGAGA AAACCAACTC CATGGATGCT
    GCAAATACCT CTCCTTTCAA AGTATCGGGC TTCTTCAGTA AACGTCTGAA AGGGTCCATC
    AAGAGGACAA AGAGTCAGTC AAAGCTGGAC AGGAACACAA GTTTCCGCCT CCCATCCCTC
    CGCAATGCTG ATGACAGGTC ACGTGGGCTG CCGAAGCTGA AGGAGTCCCG CTCTCATGAG
    TCCCTGCTGA GCCCCGGCAG CGCAGTGGAA GCCCTGGACC TTGGGGCAGA AGAAAAGGTC
    TTTGTAAAGC CACTGCACAG CAGCATCCTC GGACAGGACT TCTGCTTTGA GGTAACCTAC
    TCCAGCGGCA GTAAATGCTT CAGCTGTTCC TCTGCTGCAG AGAGGGATAA GTGGATGGAA
    AACCTACGCA GAACAGTGCA GCCGAATAAG GACAACTGCC GTCGAGCTGA AAATGTGCTC
    CGTCTCTGGA TCATTGAGGC AAAGGACCTG GCCCCCAAGA AGAAATACTT CTGTGAGCTG
    TGCCTTGATG ACACTCTCTT TGCCCGCACC ACCAGCAAAA CGAAAGCAGA TAACATTTTC
    TGGGGGGAGC ACTTTGAGTT CTACGGCCTC CCAGCTCTTC ACAGCATCAC GGTTCACATC
    TACAAGGATG TGGAGAAGAA AAAGAAAAAG GACAAAAACA ATTACGTCGG CCTGGTCAAC
    ATTCCCATGG CCACTGTAAC GGGGCGCCAG TTTGTGGAAA AATGGTACCC AGTCAGCACA
    CCTACACCCA ACAAAGGGAA ATCAGGAGGC CCTTCCATTC GCATCAAGTC GCGTTATCAG
    ACCATCACCA TCTTACCCAT GGAGCAATAC AAGGAGTTTG CAGAGTTTGT TACCAGCAAC
    TACACGATGC TGTGCTCTGT GCTGGAGCCA GTCATCAGCG TAAGGAATAA GGAAGAGATG
    GCTTGTGCAA TGGTGCACAT TCTTCAGAGC ACGGGGAGAG CCAAGGACTT CCTAACGGAT
    CTGGTGATGT CTGAGGTGGA TCGCTGCGGG GAACACGATG TCTTGATCTT CAGGGAGAAC
    ACACTTGCTA CCAAAGCTAT TGAGGAATAC CTGAAGTTGG TAGGGCAGAA GTATCTCCAT
    GATGCGCTAG GGGAGTTTAT CAAGGCTTTG TATGAGTCAG ATGAAAACTG TGAAGTGGAC
    CCCAGCAAAT GTTCATCCAG TGAATTAACA GACCATCAGA GCAACCTGAA AATGTGTTGT
    GAGCTGGCCT TCTGCAAGAT TATCAATTCC TACTGCGTGT TCCCTCGTGA GCTGAAGGAG
    GTGTTTGCAT CTTGGAAGCA GCAGTGCCTG AGCAGGGGCA AGCAGGACAT CAGCGAGCGG
    CTGATCAGTG CCTCCCTGTT CCTCCGCTTC CTCTGCCCCG CCATCATGTC CCCCAGCCTC
    TTCAGCCTCA TGCAGGAGTA TCCTGACGAC CGGACATCGC GCACGCTCAC ACTCATTGCT
    AAAGTCATTC AGAATCTCGC CAATTTTGCT AAGTTTGGTA ACAAGGAAGA GTACATGGCT
    TTCATGAACG ACTTTCTGGA ACATGAGTGG GGGGGAATGA AGCGATTTCT CCTGGAGATC
    TCTAATCCAG ATACCATCTC AAACATGCCT GGATTTGAGG GCTATATCGA CCTGGGCAGA
    GAGCTCTCAG TTTTGCATTC CCTCTTATGG GAGGTTGTGT CCCAACTTGA TAAGGGTGAA
    AATTCCTTCC TACAGGCGAC CGTGGCAAAA CTGGGACCTC TTCCTCGTAT TCTTGCCGAC
    ATCACCAAAT CAATGACCAA CCCTACGCCA ATACAGCAGC AGCTGAGGCG TTTCACTGAG
    CACAGCTCTA GTCCAAACGT CAGTGGCAGT CTCTCCTCAG GGCTTCAGAA GATATTCGAG
    GACCCCACTG ATGGTGACTT GCATAAACTG AAGTCTCCAA CCCAGGAAAA CATAGATGGC
    TATTTCAGGG GCAAGACCTT GCTGTTGGTT CAGCAGGCGT CCACCCAGAG CATGACTTAC
    TCAGATAAGG ACGAAAGAGA GAGCATCTTG CCCAACGGAC GTAGCATCTC CCTCATGGAC
    CTGCAGGATC CCCATACCGC TCACAGCGAC CACGCTTCCA TGATGCACGA CGTGCCTTTG
    CGCTTGGCCG GCAGCCAGCT CTCCATCACC CAGGTGGCCA ACATCAAGCA GCTGTGGGAG
    ACCCAGAGCA CGCCCCAAAG CGCTCCCCAG GTGAGGAGGC CGCTGCACCC GGCTTTGAAC
    CAGCAGGGCA GCCTCCAGCC TCTGTCATTC CAGAACCCGG TGTACCACCT CAACAACCCA
    CCTGCGCCGT TGCCCAAGGC CTCCGTGGAC TCCAGCCTGG AGAACCTGAG CACCGCCAGC
    TCCCAGAGCC GAAGCAACAG CGAAGACTTC AAGCTCAGCG GGCCCAGCAA CAGCAGCATG
    GAGGACTTCA CCAAGCGCAG CACGCAGAGC GAGGACTTCT CTAGGCGGCA CACGGTCCCG
    GACAAGCACG TCCCCATCGC GTTGCCCCGC CAGAACAGCA GCAGCCAGGC ACAGATCCGC
    AAAATGGACC AGGCCGGGTT AGGGGCCAGG GCCAAAGCGC CGCAGTCGCT GCCGCACAGC
    GCGTCCCTGC GCAGCACGGG CAGCATGTCG GCCGTGTCGG GGGCCGTGGT GACTGAGCCC
    GTCCAGAACG GCAGCCGCTC CCGGCAGCAG TCGTCCTCCT CCAGGGAGAG CCCTGTGCCG
    AAGGTGAGGG CCATTCAGAG GCAGCAAACG CAGCAGGTCC AGTCTCCTGT GGACTCAGCC
    ACAATGTCAC CAGTGGAAAG GACAGCAGCG TGGGTTCTCA ATAACGGGCA GTACGAGGAG
    GTCGAGGAGG ACACAGAGCA GAACCCAGAT GAAGCCAAGC ATGCTGAGAA GTACGAACAA
    GAGATAGCAA AGCTGAAGGA ACGCTTGAAA GTGTCGAGCC GCCGGCTGGA GGAGTACGAG
    CGGCGGCTCC TGGTGCAGGA GCAGCAAATG CAGAAGCTGC TGATGGAGTA CAAGTCCAGG
    CTGGAGGACA GCGAGGAGAG ACTGCGCAGG CAACAGGAGG AGAAGGACAG CCAGATGAAA
    AGCATTATCA GCAGGCTCAT GGCAGTTGAA GAGGAACTGA AGAAAGATCA TGCAGAGATG
    CAAGCTGTCA TCGATGCAAA GCAGAAAATT ATTGATGCAC AGGAGAAGCG GATCGTGTCC
    CTGGACTCTG CCAACACGCG GCTGATGAGT GCCCTGACGC AGGTGAAGGA GCGCTACAGC
    ATGCAAGTCC GCAATGGCAT CTCCCCCACC AACCCCACCA AGCTTTCCAT CACGGAGAAT
    GGTGAATTCA AAAACAGCAG CTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004943302.2
    CDS633..4919
    Translation

    Target ORF information:

    RefSeq Version XM_004943245.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004943245.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAGTGCTT TTAAAAAGTA TTACAGTCTC TCTCCCAGGA CCTTTAGGAG GTCTTTGTCG 
    GAGCAAGATG CTGGCACCGC TGCCTTAAAG GACTATAATT GGTACCACCA AATGAGAAGC
    AAGAAGGGGA GCAAACAAAC AGCTTTGGAC CGAGAGAAAG CACTCGGAGC GTACAGGTGG
    CAAACACATC CCCGTCTTCC CTGGAGGACA AGCAGAAGAG CACACTCTTG CAGCCTCAGA
    GGGGAAGGTA ATGATTCGAG TCTTGTCAAA AGTTCTGCAA GCATGGATGC TACCAGCGCC
    GACGATCTCC ACCAAGACTC AACGGAGATG CTCTTCCAGG CAAGGACCAG ATCACCGCGG
    GTTCCCTTCA ACCGCTCCCT GTCTGAACCT GTGCCGCACT CTGCAAACAG GAATGCCAAG
    CCCTTTCTGC AGGCGGTGCG CTCTGTGGAT TGTGAGCAGC ACAGCTATTT TGTCCGTGGC
    ATCTTCTCAA CTCTCTCAAG TGGCTTTTCA AAGATAATAA ATCGAAGGGG TGAGCAGCCC
    CCCACTGAGC CACTGGAGGA GTGGAAACCA GCCGGTAGTC AAATTGACCC GTGCCCAGGT
    AATGGACTCT GTTCTGTTTG TGTTACAGAT GTAAAAGGAC CACCTACGCA CAGGCTGTCC
    TGCGGCCAGT CCCCCTACAC CGAGACGACA ACATGGGAGA GGAAGTACTG CATCCTGACT
    GACAGCCAGC TGGTGCTGCT CAACAGGGAG AAGGAGGTGC CCACCGAGGG GTTGCAGGAG
    CCGCAGGATG CAACCAAAGG CCGCTGTCTG CGCCGCACGG TGAGCGTGCC CTCCGAGGGC
    CAGTTCCCCG AGTACCCACC AGATGGGGCC GCCAAGCTGG AGGTCCCGGC AGAGAGATCT
    CCTCGCAGAC GGAGCATCTC TGGGACCAGC ACATCTGAGA AAACCAACTC CATGGATGCT
    GCAAATACCT CTCCTTTCAA AGTATCGGGC TTCTTCAGTA AACGTCTGAA AGGGTCCATC
    AAGAGGACAA AGAGTCAGTC AAAGCTGGAC AGGAACACAA GTTTCCGCCT CCCATCCCTC
    CGCAATGCTG ATGACAGGTC ACGTGGGCTG CCGAAGCTGA AGGAGTCCCG CTCTCATGAG
    TCCCTGCTGA GCCCCGGCAG CGCAGTGGAA GCCCTGGACC TTGGGGCAGA AGAAAAGGTC
    TTTGTAAAGC CACTGCACAG CAGCATCCTC GGACAGGACT TCTGCTTTGA GGTAACCTAC
    TCCAGCGGCA GTAAATGCTT CAGCTGTTCC TCTGCTGCAG AGAGGGATAA GTGGATGGAA
    AACCTACGCA GAACAGTGCA GCCGAATAAG GACAACTGCC GTCGAGCTGA AAATGTGCTC
    CGTCTCTGGA TCATTGAGGC AAAGGACCTG GCCCCCAAGA AGAAATACTT CTGTGAGCTG
    TGCCTTGATG ACACTCTCTT TGCCCGCACC ACCAGCAAAA CGAAAGCAGA TAACATTTTC
    TGGGGGGAGC ACTTTGAGTT CTACGGCCTC CCAGCTCTTC ACAGCATCAC GGTTCACATC
    TACAAGGATG TGGAGAAGAA AAAGAAAAAG GACAAAAACA ATTACGTCGG CCTGGTCAAC
    ATTCCCATGG CCACTGTAAC GGGGCGCCAG TTTGTGGAAA AATGGTACCC AGTCAGCACA
    CCTACACCCA ACAAAGGGAA ATCAGGAGGC CCTTCCATTC GCATCAAGTC GCGTTATCAG
    ACCATCACCA TCTTACCCAT GGAGCAATAC AAGGAGTTTG CAGAGTTTGT TACCAGCAAC
    TACACGATGC TGTGCTCTGT GCTGGAGCCA GTCATCAGCG TAAGGAATAA GGAAGAGATG
    GCTTGTGCAA TGGTGCACAT TCTTCAGAGC ACGGGGAGAG CCAAGGACTT CCTAACGGAT
    CTGGTGATGT CTGAGGTGGA TCGCTGCGGG GAACACGATG TCTTGATCTT CAGGGAGAAC
    ACACTTGCTA CCAAAGCTAT TGAGGAATAC CTGAAGTTGG TAGGGCAGAA GTATCTCCAT
    GATGCGCTAG GGGAGTTTAT CAAGGCTTTG TATGAGTCAG ATGAAAACTG TGAAGTGGAC
    CCCAGCAAAT GTTCATCCAG TGAATTAACA GACCATCAGA GCAACCTGAA AATGTGTTGT
    GAGCTGGCCT TCTGCAAGAT TATCAATTCC TACTGCGTGT TCCCTCGTGA GCTGAAGGAG
    GTGTTTGCAT CTTGGAAGCA GCAGTGCCTG AGCAGGGGCA AGCAGGACAT CAGCGAGCGG
    CTGATCAGTG CCTCCCTGTT CCTCCGCTTC CTCTGCCCCG CCATCATGTC CCCCAGCCTC
    TTCAGCCTCA TGCAGGAGTA TCCTGACGAC CGGACATCGC GCACGCTCAC ACTCATTGCT
    AAAGTCATTC AGAATCTCGC CAATTTTGCT AAGTTTGGTA ACAAGGAAGA GTACATGGCT
    TTCATGAACG ACTTTCTGGA ACATGAGTGG GGGGGAATGA AGCGATTTCT CCTGGAGATC
    TCTAATCCAG ATACCATCTC AAACATGCCT GGATTTGAGG GCTATATCGA CCTGGGCAGA
    GAGCTCTCAG TTTTGCATTC CCTCTTATGG GAGGTTGTGT CCCAACTTGA TAAGGGTGAA
    AATTCCTTCC TACAGGCGAC CGTGGCAAAA CTGGGACCTC TTCCTCGTAT TCTTGCCGAC
    ATCACCAAAT CAATGACCAA CCCTACGCCA ATACAGCAGC AGCTGAGGCG TTTCACTGAG
    CACAGCTCTA GTCCAAACGT CAGTGGCAGT CTCTCCTCAG GGCTTCAGAA GATATTCGAG
    GACCCCACTG ATGGTGACTT GCATAAACTG AAGTCTCCAA CCCAGGAAAA CATAGATGGC
    TATTTCAGGG GCAAGACCTT GCTGTTGGTT CAGCAGGCGT CCACCCAGAG CATGACTTAC
    TCAGATAAGG ACGAAAGAGA GAGCATCTTG CCCAACGGAC GTAGCATCTC CCTCATGGAC
    CTGCAGGATC CCCATACCGC TCACAGCGAC CACGCTTCCA TGATGCACGA CGTGCCTTTG
    CGCTTGGCCG GCAGCCAGCT CTCCATCACC CAGGTGGCCA ACATCAAGCA GCTGTGGGAG
    ACCCAGAGCA CGCCCCAAAG CGCTCCCCAG GTGAGGAGGC CGCTGCACCC GGCTTTGAAC
    CAGCAGGGCA GCCTCCAGCC TCTGTCATTC CAGAACCCGG TGTACCACCT CAACAACCCA
    CCTGCGCCGT TGCCCAAGGC CTCCGTGGAC TCCAGCCTGG AGAACCTGAG CACCGCCAGC
    TCCCAGAGCC GAAGCAACAG CGAAGACTTC AAGCTCAGCG GGCCCAGCAA CAGCAGCATG
    GAGGACTTCA CCAAGCGCAG CACGCAGAGC GAGGACTTCT CTAGGCGGCA CACGGTCCCG
    GACAAGCACG TCCCCATCGC GTTGCCCCGC CAGAACAGCA GCAGCCAGGC ACAGATCCGC
    AAAATGGACC AGGCCGGGTT AGGGGCCAGG GCCAAAGCGC CGCAGTCGCT GCCGCACAGC
    GCGTCCCTGC GCAGCACGGG CAGCATGTCG GCCGTGTCGG GGGCCGTGGT GACTGAGCCC
    GTCCAGAACG GCAGCCGCTC CCGGCAGCAG TCGTCCTCCT CCAGGGAGAG CCCTGTGCCG
    AAGGTGAGGG CCATTCAGAG GCAGCAAACG CAGCAGGTCC AGTCTCCTGT GGACTCAGCC
    ACAATGTCAC CAGTGGAAAG GACAGCAGCG TGGGTTCTCA ATAACGGGCA GTACGAGGAG
    GTCGAGGAGG ACACAGAGCA GAACCCAGAT GAAGCCAAGC ATGCTGAGAA GTACGAACAA
    GAGATAGCAA AGCTGAAGGA ACGCTTGAAA GTGTCGAGCC GCCGGCTGGA GGAGTACGAG
    CGGCGGCTCC TGGTGCAGGA GCAGCAAATG CAGAAGCTGC TGATGGAGTA CAAGTCCAGG
    CTGGAGGACA GCGAGGAGAG ACTGCGCAGG CAACAGGAGG AGAAGGACAG CCAGATGAAA
    AGCATTATCA GCAGGCTCAT GGCAGTTGAA GAGGAACTGA AGAAAGATCA TGCAGAGATG
    CAAGCTGTCA TCGATGCAAA GCAGAAAATT ATTGATGCAC AGGAGAAGCG GATCGTGTCC
    CTGGACTCTG CCAACACGCG GCTGATGAGT GCCCTGACGC AGGTGAAGGA GCGCTACAGC
    ATGCAAGTCC GCAATGGCAT CTCCCCCACC AACCCCACCA AGCTTTCCAT CACGGAGAAT
    GGTGAATTCA AAAACAGCAG CTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39041
    Clone ID Related Accession (Same CDS sequence) XM_015290511.1
    Accession Version XM_015290511.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4275bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product ras GTPase-activating protein nGAP isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAGTGCTT TTAAAAAGTA TTACAGTCTC TCTCCCAGGA CCTTTAGGAG GTCTTTGTCG 
    GAGCAAGATG CTGGCACCGC TGCCTTAAAG GACTATAATT GGTACCACCA AATGAGAAGC
    AAGAAGGGGA GCAAACAAAC AGCTTTGGAC CGAGAGAAAG CACTCGGAGC GTACAGGTGG
    CAAACACATC CCCGTCTTCC CTGGAGGACA AGCAGAAGAG CACACTCTTG CAGCCTCAGA
    GGGGAAGGTA ATGATTCGAG TCTTGTCAAA AGTTCTGCAA GCATGGATGC TACCAGCGCC
    GACGATCTCC ACCAAGACTC AACGGAGATG CTCTTCCAGG CAAGGACCAG ATCACCGCGG
    GTTCCCTTCA ACCGCTCCCT GTCTGAACCT GTGCCGCACT CTGCAAACAG GAATGCCAAG
    CCCTTTCTGC AGGCGGTGCG CTCTGTGGAT TGTGAGCAGC ACAGCTATTT TGTCCGTGGC
    ATCTTCTCAA CTCTCTCAAG TGGCTTTTCA AAGATAATAA ATCGAAGGGG TGAGCAGCCC
    CCCACTGAGC CACTGGAGGA GTGGAAACCA GCCGGTAGTC AAATTGACCC GTGCCCAGGT
    AATGGACTCT GTTCTGTTTG TGTTACAGAT GTAAAAGGAC CACCTACGCA CAGGCTGTCC
    TGCGGCCAGT CCCCCTACAC CGAGACGACA ACATGGGAGA GGAAGTACTG CATCCTGACT
    GACAGCCAGC TGGTGCTGCT CAACAGGGAG AAGGAGGTGC CCACCGAGGG GTTGCAGGAG
    CCGCAGGATG CAACCAAAGG CCGCTGTCTG CGCCGCACGG TGAGCGTGCC CTCCGAGGGC
    CAGTTCCCCG AGTACCCACC AGATGGGGCC GCCAAGCTGG AGGTCCCGGC AGAGAGATCT
    CCTCGCAGAC GGAGCATCTC TGGGACCAGC ACATCTGAGA AAACCAACTC CATGGATGCT
    GCAAATACCT CTCCTTTCAA AGTATCGGGC TTCTTCAGTA AACGTCTGAA AGGGTCCATC
    AAGAGGACAA AGAGTCAGTC AAAGCTGGAC AGGAACACAA GTTTCCGCCT CCCATCCCTC
    CGCAATGCTG ATGACAGGTC ACGTGGGCTG CCGAAGCTGA AGGAGTCCCG CTCTCATGAG
    TCCCTGCTGA GCCCCGGCAG CGCAGTGGAA GCCCTGGACC TTGGGGCAGA AGAAAAGGTC
    TTTGTAAAGC CACTGCACAG CAGCATCCTC GGACAGGACT TCTGCTTTGA GGTAACCTAC
    TCCAGCGGCA GTAAATGCTT CAGCTGTTCC TCTGCTGCAG AGAGGGATAA GTGGATGGAA
    AACCTACGCA GAACAGTGCA GCCGAATAAG GACAACTGCC GTCGAGCTGA AAATGTGCTC
    CGTCTCTGGA TCATTGAGGC AAAGGACCTG GCCCCCAAGA AGAAATACTT CTGTGAGCTG
    TGCCTTGATG ACACTCTCTT TGCCCGCACC ACCAGCAAAA CGAAAGCAGA TAACATTTTC
    TGGGGGGAGC ACTTTGAGTT CTACGGCCTC CCAGCTCTTC ACAGCATCAC GGTTCACATC
    TACAAGGATG TGGAGAAGAA AAAGAAAAAG GACAAAAACA ATTACGTCGG CCTGGTCAAC
    ATTCCCATGG CCACTGTAAC GGGGCGCCAG TTTGTGGAAA AATGGTACCC AGTCAGCACA
    CCTACACCCA ACAAAGGGAA ATCAGGAGGC CCTTCCATTC GCATCAAGTC GCGTTATCAG
    ACCATCACCA TCTTACCCAT GGAGCAATAC AAGGAGTTTG CAGAGTTTGT TACCAGCAAC
    TACACGATGC TGTGCTCTGT GCTGGAGCCA GTCATCAGCG TAAGGAATAA GGAAGAGATG
    GCTTGTGCAA TGGTGCACAT TCTTCAGAGC ACGGGGAGAG CCAAGGACTT CCTAACGGAT
    CTGGTGATGT CTGAGGTGGA TCGCTGCGGG GAACACGATG TCTTGATCTT CAGGGAGAAC
    ACACTTGCTA CCAAAGCTAT TGAGGAATAC CTGAAGTTGG TAGGGCAGAA GTATCTCCAT
    GATGCGCTAG GGGAGTTTAT CAAGGCTTTG TATGAGTCAG ATGAAAACTG TGAAGTGGAC
    CCCAGCAAAT GTTCATCCAG TGAATTAACA GACCATCAGA GCAACCTGAA AATGTGTTGT
    GAGCTGGCCT TCTGCAAGAT TATCAATTCC TACTGCGTGT TCCCTCGTGA GCTGAAGGAG
    GTGTTTGCAT CTTGGAAGCA GCAGTGCCTG AGCAGGGGCA AGCAGGACAT CAGCGAGCGG
    CTGATCAGTG CCTCCCTGTT CCTCCGCTTC CTCTGCCCCG CCATCATGTC CCCCAGCCTC
    TTCAGCCTCA TGCAGGAGTA TCCTGACGAC CGGACATCGC GCACGCTCAC ACTCATTGCT
    AAAGTCATTC AGAATCTCGC CAATTTTGCT AAGTTTGGTA ACAAGGAAGA GTACATGGCT
    TTCATGAACG ACTTTCTGGA ACATGAGTGG GGGGGAATGA AGCGATTTCT CCTGGAGATC
    TCTAATCCAG ATACCATCTC AAACATGCCT GGATTTGAGG GCTATATCGA CCTGGGCAGA
    GAGCTCTCAG TTTTGCATTC CCTCTTATGG GAGGTTGTGT CCCAACTTGA TAAGGCGACC
    GTGGCAAAAC TGGGACCTCT TCCTCGTATT CTTGCCGACA TCACCAAATC AATGACCAAC
    CCTACGCCAA TACAGCAGCA GCTGAGGCGT TTCACTGAGC ACAGCTCTAG TCCAAACGTC
    AGTGGCAGTC TCTCCTCAGG GCTTCAGAAG ATATTCGAGG ACCCCACTGA TGGTGACTTG
    CATAAACTGA AGTCTCCAAC CCAGGAAAAC ATAGATGGCT ATTTCAGGGG CAAGACCTTG
    CTGTTGGTTC AGCAGGCGTC CACCCAGAGC ATGACTTACT CAGATAAGGA CGAAAGAGAG
    AGCATCTTGC CCAACGGACG TAGCATCTCC CTCATGGACC TGCAGGATCC CCATACCGCT
    CACAGCGACC ACGCTTCCAT GATGCACGAC GTGCCTTTGC GCTTGGCCGG CAGCCAGCTC
    TCCATCACCC AGGTGGCCAA CATCAAGCAG CTGTGGGAGA CCCAGAGCAC GCCCCAAAGC
    GCTCCCCAGG TGAGGAGGCC GCTGCACCCG GCTTTGAACC AGCAGGGCAG CCTCCAGCCT
    CTGTCATTCC AGAACCCGGT GTACCACCTC AACAACCCAC CTGCGCCGTT GCCCAAGGCC
    TCCGTGGACT CCAGCCTGGA GAACCTGAGC ACCGCCAGCT CCCAGAGCCG AAGCAACAGC
    GAAGACTTCA AGCTCAGCGG GCCCAGCAAC AGCAGCATGG AGGACTTCAC CAAGCGCAGC
    ACGCAGAGCG AGGACTTCTC TAGGCGGCAC ACGGTCCCGG ACAAGCACGT CCCCATCGCG
    TTGCCCCGCC AGAACAGCAG CAGCCAGGCA CAGATCCGCA AAATGGACCA GGCCGGGTTA
    GGGGCCAGGG CCAAAGCGCC GCAGTCGCTG CCGCACAGCG CGTCCCTGCG CAGCACGGGC
    AGCATGTCGG CCGTGTCGGG GGCCGTGGTG ACTGAGCCCG TCCAGAACGG CAGCCGCTCC
    CGGCAGCAGT CGTCCTCCTC CAGGGAGAGC CCTGTGCCGA AGGTGAGGGC CATTCAGAGG
    CAGCAAACGC AGCAGGTCCA GTCTCCTGTG GACTCAGCCA CAATGTCACC AGTGGAAAGG
    ACAGCAGCGT GGGTTCTCAA TAACGGGCAG TACGAGGAGG TCGAGGAGGA CACAGAGCAG
    AACCCAGATG AAGCCAAGCA TGCTGAGAAG TACGAACAAG AGATAGCAAA GCTGAAGGAA
    CGCTTGAAAG TGTCGAGCCG CCGGCTGGAG GAGTACGAGC GGCGGCTCCT GGTGCAGGAG
    CAGCAAATGC AGAAGCTGCT GATGGAGTAC AAGTCCAGGC TGGAGGACAG CGAGGAGAGA
    CTGCGCAGGC AACAGGAGGA GAAGGACAGC CAGATGAAAA GCATTATCAG CAGGCTCATG
    GCAGTTGAAG AGGAACTGAA GAAAGATCAT GCAGAGATGC AAGCTGTCAT CGATGCAAAG
    CAGAAAATTA TTGATGCACA GGTCATTAAT GGGAATGAGA TAATTCAAAC AGGAGAAGCG
    GATCGTGTCC CTGGACTCTG CCAACACGCG GCTGATGAGT GCCCTGACGC AGGTGAAGGA
    GCGCTACAGC ATGCAAGTCC GCAATGGCAT CTCCCCCACC AACCCCACCA AGCTTTCCAT
    CACGGAGAAT GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015145997.1
    CDS638..4912
    Translation

    Target ORF information:

    RefSeq Version XM_015290511.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015290511.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAGTGCTT TTAAAAAGTA TTACAGTCTC TCTCCCAGGA CCTTTAGGAG GTCTTTGTCG 
    GAGCAAGATG CTGGCACCGC TGCCTTAAAG GACTATAATT GGTACCACCA AATGAGAAGC
    AAGAAGGGGA GCAAACAAAC AGCTTTGGAC CGAGAGAAAG CACTCGGAGC GTACAGGTGG
    CAAACACATC CCCGTCTTCC CTGGAGGACA AGCAGAAGAG CACACTCTTG CAGCCTCAGA
    GGGGAAGGTA ATGATTCGAG TCTTGTCAAA AGTTCTGCAA GCATGGATGC TACCAGCGCC
    GACGATCTCC ACCAAGACTC AACGGAGATG CTCTTCCAGG CAAGGACCAG ATCACCGCGG
    GTTCCCTTCA ACCGCTCCCT GTCTGAACCT GTGCCGCACT CTGCAAACAG GAATGCCAAG
    CCCTTTCTGC AGGCGGTGCG CTCTGTGGAT TGTGAGCAGC ACAGCTATTT TGTCCGTGGC
    ATCTTCTCAA CTCTCTCAAG TGGCTTTTCA AAGATAATAA ATCGAAGGGG TGAGCAGCCC
    CCCACTGAGC CACTGGAGGA GTGGAAACCA GCCGGTAGTC AAATTGACCC GTGCCCAGGT
    AATGGACTCT GTTCTGTTTG TGTTACAGAT GTAAAAGGAC CACCTACGCA CAGGCTGTCC
    TGCGGCCAGT CCCCCTACAC CGAGACGACA ACATGGGAGA GGAAGTACTG CATCCTGACT
    GACAGCCAGC TGGTGCTGCT CAACAGGGAG AAGGAGGTGC CCACCGAGGG GTTGCAGGAG
    CCGCAGGATG CAACCAAAGG CCGCTGTCTG CGCCGCACGG TGAGCGTGCC CTCCGAGGGC
    CAGTTCCCCG AGTACCCACC AGATGGGGCC GCCAAGCTGG AGGTCCCGGC AGAGAGATCT
    CCTCGCAGAC GGAGCATCTC TGGGACCAGC ACATCTGAGA AAACCAACTC CATGGATGCT
    GCAAATACCT CTCCTTTCAA AGTATCGGGC TTCTTCAGTA AACGTCTGAA AGGGTCCATC
    AAGAGGACAA AGAGTCAGTC AAAGCTGGAC AGGAACACAA GTTTCCGCCT CCCATCCCTC
    CGCAATGCTG ATGACAGGTC ACGTGGGCTG CCGAAGCTGA AGGAGTCCCG CTCTCATGAG
    TCCCTGCTGA GCCCCGGCAG CGCAGTGGAA GCCCTGGACC TTGGGGCAGA AGAAAAGGTC
    TTTGTAAAGC CACTGCACAG CAGCATCCTC GGACAGGACT TCTGCTTTGA GGTAACCTAC
    TCCAGCGGCA GTAAATGCTT CAGCTGTTCC TCTGCTGCAG AGAGGGATAA GTGGATGGAA
    AACCTACGCA GAACAGTGCA GCCGAATAAG GACAACTGCC GTCGAGCTGA AAATGTGCTC
    CGTCTCTGGA TCATTGAGGC AAAGGACCTG GCCCCCAAGA AGAAATACTT CTGTGAGCTG
    TGCCTTGATG ACACTCTCTT TGCCCGCACC ACCAGCAAAA CGAAAGCAGA TAACATTTTC
    TGGGGGGAGC ACTTTGAGTT CTACGGCCTC CCAGCTCTTC ACAGCATCAC GGTTCACATC
    TACAAGGATG TGGAGAAGAA AAAGAAAAAG GACAAAAACA ATTACGTCGG CCTGGTCAAC
    ATTCCCATGG CCACTGTAAC GGGGCGCCAG TTTGTGGAAA AATGGTACCC AGTCAGCACA
    CCTACACCCA ACAAAGGGAA ATCAGGAGGC CCTTCCATTC GCATCAAGTC GCGTTATCAG
    ACCATCACCA TCTTACCCAT GGAGCAATAC AAGGAGTTTG CAGAGTTTGT TACCAGCAAC
    TACACGATGC TGTGCTCTGT GCTGGAGCCA GTCATCAGCG TAAGGAATAA GGAAGAGATG
    GCTTGTGCAA TGGTGCACAT TCTTCAGAGC ACGGGGAGAG CCAAGGACTT CCTAACGGAT
    CTGGTGATGT CTGAGGTGGA TCGCTGCGGG GAACACGATG TCTTGATCTT CAGGGAGAAC
    ACACTTGCTA CCAAAGCTAT TGAGGAATAC CTGAAGTTGG TAGGGCAGAA GTATCTCCAT
    GATGCGCTAG GGGAGTTTAT CAAGGCTTTG TATGAGTCAG ATGAAAACTG TGAAGTGGAC
    CCCAGCAAAT GTTCATCCAG TGAATTAACA GACCATCAGA GCAACCTGAA AATGTGTTGT
    GAGCTGGCCT TCTGCAAGAT TATCAATTCC TACTGCGTGT TCCCTCGTGA GCTGAAGGAG
    GTGTTTGCAT CTTGGAAGCA GCAGTGCCTG AGCAGGGGCA AGCAGGACAT CAGCGAGCGG
    CTGATCAGTG CCTCCCTGTT CCTCCGCTTC CTCTGCCCCG CCATCATGTC CCCCAGCCTC
    TTCAGCCTCA TGCAGGAGTA TCCTGACGAC CGGACATCGC GCACGCTCAC ACTCATTGCT
    AAAGTCATTC AGAATCTCGC CAATTTTGCT AAGTTTGGTA ACAAGGAAGA GTACATGGCT
    TTCATGAACG ACTTTCTGGA ACATGAGTGG GGGGGAATGA AGCGATTTCT CCTGGAGATC
    TCTAATCCAG ATACCATCTC AAACATGCCT GGATTTGAGG GCTATATCGA CCTGGGCAGA
    GAGCTCTCAG TTTTGCATTC CCTCTTATGG GAGGTTGTGT CCCAACTTGA TAAGGCGACC
    GTGGCAAAAC TGGGACCTCT TCCTCGTATT CTTGCCGACA TCACCAAATC AATGACCAAC
    CCTACGCCAA TACAGCAGCA GCTGAGGCGT TTCACTGAGC ACAGCTCTAG TCCAAACGTC
    AGTGGCAGTC TCTCCTCAGG GCTTCAGAAG ATATTCGAGG ACCCCACTGA TGGTGACTTG
    CATAAACTGA AGTCTCCAAC CCAGGAAAAC ATAGATGGCT ATTTCAGGGG CAAGACCTTG
    CTGTTGGTTC AGCAGGCGTC CACCCAGAGC ATGACTTACT CAGATAAGGA CGAAAGAGAG
    AGCATCTTGC CCAACGGACG TAGCATCTCC CTCATGGACC TGCAGGATCC CCATACCGCT
    CACAGCGACC ACGCTTCCAT GATGCACGAC GTGCCTTTGC GCTTGGCCGG CAGCCAGCTC
    TCCATCACCC AGGTGGCCAA CATCAAGCAG CTGTGGGAGA CCCAGAGCAC GCCCCAAAGC
    GCTCCCCAGG TGAGGAGGCC GCTGCACCCG GCTTTGAACC AGCAGGGCAG CCTCCAGCCT
    CTGTCATTCC AGAACCCGGT GTACCACCTC AACAACCCAC CTGCGCCGTT GCCCAAGGCC
    TCCGTGGACT CCAGCCTGGA GAACCTGAGC ACCGCCAGCT CCCAGAGCCG AAGCAACAGC
    GAAGACTTCA AGCTCAGCGG GCCCAGCAAC AGCAGCATGG AGGACTTCAC CAAGCGCAGC
    ACGCAGAGCG AGGACTTCTC TAGGCGGCAC ACGGTCCCGG ACAAGCACGT CCCCATCGCG
    TTGCCCCGCC AGAACAGCAG CAGCCAGGCA CAGATCCGCA AAATGGACCA GGCCGGGTTA
    GGGGCCAGGG CCAAAGCGCC GCAGTCGCTG CCGCACAGCG CGTCCCTGCG CAGCACGGGC
    AGCATGTCGG CCGTGTCGGG GGCCGTGGTG ACTGAGCCCG TCCAGAACGG CAGCCGCTCC
    CGGCAGCAGT CGTCCTCCTC CAGGGAGAGC CCTGTGCCGA AGGTGAGGGC CATTCAGAGG
    CAGCAAACGC AGCAGGTCCA GTCTCCTGTG GACTCAGCCA CAATGTCACC AGTGGAAAGG
    ACAGCAGCGT GGGTTCTCAA TAACGGGCAG TACGAGGAGG TCGAGGAGGA CACAGAGCAG
    AACCCAGATG AAGCCAAGCA TGCTGAGAAG TACGAACAAG AGATAGCAAA GCTGAAGGAA
    CGCTTGAAAG TGTCGAGCCG CCGGCTGGAG GAGTACGAGC GGCGGCTCCT GGTGCAGGAG
    CAGCAAATGC AGAAGCTGCT GATGGAGTAC AAGTCCAGGC TGGAGGACAG CGAGGAGAGA
    CTGCGCAGGC AACAGGAGGA GAAGGACAGC CAGATGAAAA GCATTATCAG CAGGCTCATG
    GCAGTTGAAG AGGAACTGAA GAAAGATCAT GCAGAGATGC AAGCTGTCAT CGATGCAAAG
    CAGAAAATTA TTGATGCACA GGTCATTAAT GGGAATGAGA TAATTCAAAC AGGAGAAGCG
    GATCGTGTCC CTGGACTCTG CCAACACGCG GCTGATGAGT GCCCTGACGC AGGTGAAGGA
    GCGCTACAGC ATGCAAGTCC GCAATGGCAT CTCCCCCACC AACCCCACCA AGCTTTCCAT
    CACGGAGAAT GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39042
    Clone ID Related Accession (Same CDS sequence) XM_004943246.2
    Accession Version XM_004943246.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4266bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product ras GTPase-activating protein nGAP isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004943246.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAGTGCTT TTAAAAAGTA TTACAGTCTC TCTCCCAGGA CCTTTAGGAG GTCTTTGTCG 
    GAGCAAGATG CTGGCACCGC TGCCTTAAAG GACTATAATT GGTACCACCA AATGAGAAGC
    AAGAAGGGGA GCAAACAAAC AGCTTTGGAC CGAGAGAAAG CACTCGGAGC GTACAGGTGG
    CAAACACATC CCCGTCTTCC CTGGAGGACA AGCAGAAGAG CACACTCTTG CAGCCTCAGA
    GGGGAAGGTA ATGATTCGAG TCTTGTCAAA AGTTCTGCAA GCATGGATGC TACCAGCGCC
    GACGATCTCC ACCAAGACTC AACGGAGATG CTCTTCCAGG CAAGGACCAG ATCACCGCGG
    GTTCCCTTCA ACCGCTCCCT GTCTGAACCT GTGCCGCACT CTGCAAACAG GAATGCCAAG
    CCCTTTCTGC AGGCGGTGCG CTCTGTGGAT TGTGAGCAGC ACAGCTATTT TGTCCGTGGC
    ATCTTCTCAA CTCTCTCAAG TGGCTTTTCA AAGATAATAA ATCGAAGGGG TGAGCAGCCC
    CCCACTGAGC CACTGGAGGA GTGGAAACCA GCCGGTAGTC AAATTGACCC GTGCCCAGGT
    AATGGACTCT GTTCTGTTTG TGTTACAGAT GTAAAAGGAC CACCTACGCA CAGGCTGTCC
    TGCGGCCAGT CCCCCTACAC CGAGACGACA ACATGGGAGA GGAAGTACTG CATCCTGACT
    GACAGCCAGC TGGTGCTGCT CAACAGGGAG AAGGAGGTGC CCACCGAGGG GTTGCAGGAG
    CCGCAGGATG CAACCAAAGG CCGCTGTCTG CGCCGCACGG TGAGCGTGCC CTCCGAGGGC
    CAGTTCCCCG AGTACCCACC AGATGGGGCC GCCAAGCTGG AGGTCCCGGC AGAGAGATCT
    CCTCGCAGAC GGAGCATCTC TGGGACCAGC ACATCTGAGA AAACCAACTC CATGGATGCT
    GCAAATACCT CTCCTTTCAA AGTATCGGGC TTCTTCAGTA AACGTCTGAA AGGGTCCATC
    AAGAGGACAA AGAGTCAGTC AAAGCTGGAC AGGAACACAA GTTTCCGCCT CCCATCCCTC
    CGCAATGCTG ATGACAGGTC ACGTGGGCTG CCGAAGCTGA AGGAGTCCCG CTCTCATGAG
    TCCCTGCTGA GCCCCGGCAG CGCAGTGGAA GCCCTGGACC TTGGGGCAGA AGAAAAGGTC
    TTTGTAAAGC CACTGCACAG CAGCATCCTC GGACAGGACT TCTGCTTTGA GGTAACCTAC
    TCCAGCGGCA GTAAATGCTT CAGCTGTTCC TCTGCTGCAG AGAGGGATAA GTGGATGGAA
    AACCTACGCA GAACAGTGCA GCCGAATAAG GACAACTGCC GTCGAGCTGA AAATGTGCTC
    CGTCTCTGGA TCATTGAGGC AAAGGACCTG GCCCCCAAGA AGAAATACTT CTGTGAGCTG
    TGCCTTGATG ACACTCTCTT TGCCCGCACC ACCAGCAAAA CGAAAGCAGA TAACATTTTC
    TGGGGGGAGC ACTTTGAGTT CTACGGCCTC CCAGCTCTTC ACAGCATCAC GGTTCACATC
    TACAAGGATG TGGAGAAGAA AAAGAAAAAG GACAAAAACA ATTACGTCGG CCTGGTCAAC
    ATTCCCATGG CCACTGTAAC GGGGCGCCAG TTTGTGGAAA AATGGTACCC AGTCAGCACA
    CCTACACCCA ACAAAGGGAA ATCAGGAGGC CCTTCCATTC GCATCAAGTC GCGTTATCAG
    ACCATCACCA TCTTACCCAT GGAGCAATAC AAGGAGTTTG CAGAGTTTGT TACCAGCAAC
    TACACGATGC TGTGCTCTGT GCTGGAGCCA GTCATCAGCG TAAGGAATAA GGAAGAGATG
    GCTTGTGCAA TGGTGCACAT TCTTCAGAGC ACGGGGAGAG CCAAGGACTT CCTAACGGAT
    CTGGTGATGT CTGAGGTGGA TCGCTGCGGG GAACACGATG TCTTGATCTT CAGGGAGAAC
    ACACTTGCTA CCAAAGCTAT TGAGGAATAC CTGAAGTTGG TAGGGCAGAA GTATCTCCAT
    GATGCGCTAG GGGAGTTTAT CAAGGCTTTG TATGAGTCAG ATGAAAACTG TGAAGTGGAC
    CCCAGCAAAT GTTCATCCAG TGAATTAACA GACCATCAGA GCAACCTGAA AATGTGTTGT
    GAGCTGGCCT TCTGCAAGAT TATCAATTCC TACTGCGTGT TCCCTCGTGA GCTGAAGGAG
    GTGTTTGCAT CTTGGAAGCA GCAGTGCCTG AGCAGGGGCA AGCAGGACAT CAGCGAGCGG
    CTGATCAGTG CCTCCCTGTT CCTCCGCTTC CTCTGCCCCG CCATCATGTC CCCCAGCCTC
    TTCAGCCTCA TGCAGGAGTA TCCTGACGAC CGGACATCGC GCACGCTCAC ACTCATTGCT
    AAAGTCATTC AGAATCTCGC CAATTTTGCT AAGTTTGGTA ACAAGGAAGA GTACATGGCT
    TTCATGAACG ACTTTCTGGA ACATGAGTGG GGGGGAATGA AGCGATTTCT CCTGGAGATC
    TCTAATCCAG ATACCATCTC AAACATGCCT GGATTTGAGG GCTATATCGA CCTGGGCAGA
    GAGCTCTCAG TTTTGCATTC CCTCTTATGG GAGGTTGTGT CCCAACTTGA TAAGGCGACC
    GTGGCAAAAC TGGGACCTCT TCCTCGTATT CTTGCCGACA TCACCAAATC AATGACCAAC
    CCTACGCCAA TACAGCAGCA GCTGAGGCGT TTCACTGAGC ACAGCTCTAG TCCAAACGTC
    AGTGGCAGTC TCTCCTCAGG GCTTCAGAAG ATATTCGAGG ACCCCACTGA TGGTGACTTG
    CATAAACTGA AGTCTCCAAC CCAGGAAAAC ATAGATGGCT ATTTCAGGGG CAAGACCTTG
    CTGTTGGTTC AGCAGGCGTC CACCCAGAGC ATGACTTACT CAGATAAGGA CGAAAGAGAG
    AGCATCTTGC CCAACGGACG TAGCATCTCC CTCATGGACC TGCAGGATCC CCATACCGCT
    CACAGCGACC ACGCTTCCAT GATGCACGAC GTGCCTTTGC GCTTGGCCGG CAGCCAGCTC
    TCCATCACCC AGGTGGCCAA CATCAAGCAG CTGTGGGAGA CCCAGAGCAC GCCCCAAAGC
    GCTCCCCAGG TGAGGAGGCC GCTGCACCCG GCTTTGAACC AGCAGGGCAG CCTCCAGCCT
    CTGTCATTCC AGAACCCGGT GTACCACCTC AACAACCCAC CTGCGCCGTT GCCCAAGGCC
    TCCGTGGACT CCAGCCTGGA GAACCTGAGC ACCGCCAGCT CCCAGAGCCG AAGCAACAGC
    GAAGACTTCA AGCTCAGCGG GCCCAGCAAC AGCAGCATGG AGGACTTCAC CAAGCGCAGC
    ACGCAGAGCG AGGACTTCTC TAGGCGGCAC ACGGTCCCGG ACAAGCACGT CCCCATCGCG
    TTGCCCCGCC AGAACAGCAG CAGCCAGGCA CAGATCCGCA AAATGGACCA GGCCGGGTTA
    GGGGCCAGGG CCAAAGCGCC GCAGTCGCTG CCGCACAGCG CGTCCCTGCG CAGCACGGGC
    AGCATGTCGG CCGTGTCGGG GGCCGTGGTG ACTGAGCCCG TCCAGAACGG CAGCCGCTCC
    CGGCAGCAGT CGTCCTCCTC CAGGGAGAGC CCTGTGCCGA AGGTGAGGGC CATTCAGAGG
    CAGCAAACGC AGCAGGTCCA GTCTCCTGTG GACTCAGCCA CAATGTCACC AGTGGAAAGG
    ACAGCAGCGT GGGTTCTCAA TAACGGGCAG TACGAGGAGG TCGAGGAGGA CACAGAGCAG
    AACCCAGATG AAGCCAAGCA TGCTGAGAAG TACGAACAAG AGATAGCAAA GCTGAAGGAA
    CGCTTGAAAG TGTCGAGCCG CCGGCTGGAG GAGTACGAGC GGCGGCTCCT GGTGCAGGAG
    CAGCAAATGC AGAAGCTGCT GATGGAGTAC AAGTCCAGGC TGGAGGACAG CGAGGAGAGA
    CTGCGCAGGC AACAGGAGGA GAAGGACAGC CAGATGAAAA GCATTATCAG CAGGCTCATG
    GCAGTTGAAG AGGAACTGAA GAAAGATCAT GCAGAGATGC AAGCTGTCAT CGATGCAAAG
    CAGAAAATTA TTGATGCACA GGAGAAGCGG ATCGTGTCCC TGGACTCTGC CAACACGCGG
    CTGATGAGTG CCCTGACGCA GGTGAAGGAG CGCTACAGCA TGCAAGTCCG CAATGGCATC
    TCCCCCACCA ACCCCACCAA GCTTTCCATC ACGGAGAATG GTGAATTCAA AAACAGCAGC
    TGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004943303.2
    CDS632..4897
    Translation

    Target ORF information:

    RefSeq Version XM_004943246.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004943246.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAGTGCTT TTAAAAAGTA TTACAGTCTC TCTCCCAGGA CCTTTAGGAG GTCTTTGTCG 
    GAGCAAGATG CTGGCACCGC TGCCTTAAAG GACTATAATT GGTACCACCA AATGAGAAGC
    AAGAAGGGGA GCAAACAAAC AGCTTTGGAC CGAGAGAAAG CACTCGGAGC GTACAGGTGG
    CAAACACATC CCCGTCTTCC CTGGAGGACA AGCAGAAGAG CACACTCTTG CAGCCTCAGA
    GGGGAAGGTA ATGATTCGAG TCTTGTCAAA AGTTCTGCAA GCATGGATGC TACCAGCGCC
    GACGATCTCC ACCAAGACTC AACGGAGATG CTCTTCCAGG CAAGGACCAG ATCACCGCGG
    GTTCCCTTCA ACCGCTCCCT GTCTGAACCT GTGCCGCACT CTGCAAACAG GAATGCCAAG
    CCCTTTCTGC AGGCGGTGCG CTCTGTGGAT TGTGAGCAGC ACAGCTATTT TGTCCGTGGC
    ATCTTCTCAA CTCTCTCAAG TGGCTTTTCA AAGATAATAA ATCGAAGGGG TGAGCAGCCC
    CCCACTGAGC CACTGGAGGA GTGGAAACCA GCCGGTAGTC AAATTGACCC GTGCCCAGGT
    AATGGACTCT GTTCTGTTTG TGTTACAGAT GTAAAAGGAC CACCTACGCA CAGGCTGTCC
    TGCGGCCAGT CCCCCTACAC CGAGACGACA ACATGGGAGA GGAAGTACTG CATCCTGACT
    GACAGCCAGC TGGTGCTGCT CAACAGGGAG AAGGAGGTGC CCACCGAGGG GTTGCAGGAG
    CCGCAGGATG CAACCAAAGG CCGCTGTCTG CGCCGCACGG TGAGCGTGCC CTCCGAGGGC
    CAGTTCCCCG AGTACCCACC AGATGGGGCC GCCAAGCTGG AGGTCCCGGC AGAGAGATCT
    CCTCGCAGAC GGAGCATCTC TGGGACCAGC ACATCTGAGA AAACCAACTC CATGGATGCT
    GCAAATACCT CTCCTTTCAA AGTATCGGGC TTCTTCAGTA AACGTCTGAA AGGGTCCATC
    AAGAGGACAA AGAGTCAGTC AAAGCTGGAC AGGAACACAA GTTTCCGCCT CCCATCCCTC
    CGCAATGCTG ATGACAGGTC ACGTGGGCTG CCGAAGCTGA AGGAGTCCCG CTCTCATGAG
    TCCCTGCTGA GCCCCGGCAG CGCAGTGGAA GCCCTGGACC TTGGGGCAGA AGAAAAGGTC
    TTTGTAAAGC CACTGCACAG CAGCATCCTC GGACAGGACT TCTGCTTTGA GGTAACCTAC
    TCCAGCGGCA GTAAATGCTT CAGCTGTTCC TCTGCTGCAG AGAGGGATAA GTGGATGGAA
    AACCTACGCA GAACAGTGCA GCCGAATAAG GACAACTGCC GTCGAGCTGA AAATGTGCTC
    CGTCTCTGGA TCATTGAGGC AAAGGACCTG GCCCCCAAGA AGAAATACTT CTGTGAGCTG
    TGCCTTGATG ACACTCTCTT TGCCCGCACC ACCAGCAAAA CGAAAGCAGA TAACATTTTC
    TGGGGGGAGC ACTTTGAGTT CTACGGCCTC CCAGCTCTTC ACAGCATCAC GGTTCACATC
    TACAAGGATG TGGAGAAGAA AAAGAAAAAG GACAAAAACA ATTACGTCGG CCTGGTCAAC
    ATTCCCATGG CCACTGTAAC GGGGCGCCAG TTTGTGGAAA AATGGTACCC AGTCAGCACA
    CCTACACCCA ACAAAGGGAA ATCAGGAGGC CCTTCCATTC GCATCAAGTC GCGTTATCAG
    ACCATCACCA TCTTACCCAT GGAGCAATAC AAGGAGTTTG CAGAGTTTGT TACCAGCAAC
    TACACGATGC TGTGCTCTGT GCTGGAGCCA GTCATCAGCG TAAGGAATAA GGAAGAGATG
    GCTTGTGCAA TGGTGCACAT TCTTCAGAGC ACGGGGAGAG CCAAGGACTT CCTAACGGAT
    CTGGTGATGT CTGAGGTGGA TCGCTGCGGG GAACACGATG TCTTGATCTT CAGGGAGAAC
    ACACTTGCTA CCAAAGCTAT TGAGGAATAC CTGAAGTTGG TAGGGCAGAA GTATCTCCAT
    GATGCGCTAG GGGAGTTTAT CAAGGCTTTG TATGAGTCAG ATGAAAACTG TGAAGTGGAC
    CCCAGCAAAT GTTCATCCAG TGAATTAACA GACCATCAGA GCAACCTGAA AATGTGTTGT
    GAGCTGGCCT TCTGCAAGAT TATCAATTCC TACTGCGTGT TCCCTCGTGA GCTGAAGGAG
    GTGTTTGCAT CTTGGAAGCA GCAGTGCCTG AGCAGGGGCA AGCAGGACAT CAGCGAGCGG
    CTGATCAGTG CCTCCCTGTT CCTCCGCTTC CTCTGCCCCG CCATCATGTC CCCCAGCCTC
    TTCAGCCTCA TGCAGGAGTA TCCTGACGAC CGGACATCGC GCACGCTCAC ACTCATTGCT
    AAAGTCATTC AGAATCTCGC CAATTTTGCT AAGTTTGGTA ACAAGGAAGA GTACATGGCT
    TTCATGAACG ACTTTCTGGA ACATGAGTGG GGGGGAATGA AGCGATTTCT CCTGGAGATC
    TCTAATCCAG ATACCATCTC AAACATGCCT GGATTTGAGG GCTATATCGA CCTGGGCAGA
    GAGCTCTCAG TTTTGCATTC CCTCTTATGG GAGGTTGTGT CCCAACTTGA TAAGGCGACC
    GTGGCAAAAC TGGGACCTCT TCCTCGTATT CTTGCCGACA TCACCAAATC AATGACCAAC
    CCTACGCCAA TACAGCAGCA GCTGAGGCGT TTCACTGAGC ACAGCTCTAG TCCAAACGTC
    AGTGGCAGTC TCTCCTCAGG GCTTCAGAAG ATATTCGAGG ACCCCACTGA TGGTGACTTG
    CATAAACTGA AGTCTCCAAC CCAGGAAAAC ATAGATGGCT ATTTCAGGGG CAAGACCTTG
    CTGTTGGTTC AGCAGGCGTC CACCCAGAGC ATGACTTACT CAGATAAGGA CGAAAGAGAG
    AGCATCTTGC CCAACGGACG TAGCATCTCC CTCATGGACC TGCAGGATCC CCATACCGCT
    CACAGCGACC ACGCTTCCAT GATGCACGAC GTGCCTTTGC GCTTGGCCGG CAGCCAGCTC
    TCCATCACCC AGGTGGCCAA CATCAAGCAG CTGTGGGAGA CCCAGAGCAC GCCCCAAAGC
    GCTCCCCAGG TGAGGAGGCC GCTGCACCCG GCTTTGAACC AGCAGGGCAG CCTCCAGCCT
    CTGTCATTCC AGAACCCGGT GTACCACCTC AACAACCCAC CTGCGCCGTT GCCCAAGGCC
    TCCGTGGACT CCAGCCTGGA GAACCTGAGC ACCGCCAGCT CCCAGAGCCG AAGCAACAGC
    GAAGACTTCA AGCTCAGCGG GCCCAGCAAC AGCAGCATGG AGGACTTCAC CAAGCGCAGC
    ACGCAGAGCG AGGACTTCTC TAGGCGGCAC ACGGTCCCGG ACAAGCACGT CCCCATCGCG
    TTGCCCCGCC AGAACAGCAG CAGCCAGGCA CAGATCCGCA AAATGGACCA GGCCGGGTTA
    GGGGCCAGGG CCAAAGCGCC GCAGTCGCTG CCGCACAGCG CGTCCCTGCG CAGCACGGGC
    AGCATGTCGG CCGTGTCGGG GGCCGTGGTG ACTGAGCCCG TCCAGAACGG CAGCCGCTCC
    CGGCAGCAGT CGTCCTCCTC CAGGGAGAGC CCTGTGCCGA AGGTGAGGGC CATTCAGAGG
    CAGCAAACGC AGCAGGTCCA GTCTCCTGTG GACTCAGCCA CAATGTCACC AGTGGAAAGG
    ACAGCAGCGT GGGTTCTCAA TAACGGGCAG TACGAGGAGG TCGAGGAGGA CACAGAGCAG
    AACCCAGATG AAGCCAAGCA TGCTGAGAAG TACGAACAAG AGATAGCAAA GCTGAAGGAA
    CGCTTGAAAG TGTCGAGCCG CCGGCTGGAG GAGTACGAGC GGCGGCTCCT GGTGCAGGAG
    CAGCAAATGC AGAAGCTGCT GATGGAGTAC AAGTCCAGGC TGGAGGACAG CGAGGAGAGA
    CTGCGCAGGC AACAGGAGGA GAAGGACAGC CAGATGAAAA GCATTATCAG CAGGCTCATG
    GCAGTTGAAG AGGAACTGAA GAAAGATCAT GCAGAGATGC AAGCTGTCAT CGATGCAAAG
    CAGAAAATTA TTGATGCACA GGAGAAGCGG ATCGTGTCCC TGGACTCTGC CAACACGCGG
    CTGATGAGTG CCCTGACGCA GGTGAAGGAG CGCTACAGCA TGCAAGTCCG CAATGGCATC
    TCCCCCACCA ACCCCACCAA GCTTTCCATC ACGGAGAATG GTGAATTCAA AAACAGCAGC
    TGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39043
    Clone ID Related Accession (Same CDS sequence) XM_015290512.1
    Accession Version XM_015290512.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4266bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product ras GTPase-activating protein nGAP isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAGTGCTT TTAAAAAGTA TTACAGTCTC TCTCCCAGGA CCTTTAGGAG GTCTTTGTCG 
    GAGCAAGATG CTGGCACCGC TGCCTTAAAG GACTATAATT GGTACCACCA AATGAGAAGC
    AAGAAGGGGA GCAAACAAAC AGCTTTGGAC CGAGAGAAAG CACTCGGAGC GTACAGGTGG
    CAAACACATC CCCGTCTTCC CTGGAGGACA AGCAGAAGAG CACACTCTTG CAGCCTCAGA
    GGGGAAGGTA ATGATTCGAG TCTTGTCAAA AGTTCTGCAA GCATGGATGC TACCAGCGCC
    GACGATCTCC ACCAAGACTC AACGGAGATG CTCTTCCAGG CAAGGACCAG ATCACCGCGG
    GTTCCCTTCA ACCGCTCCCT GTCTGAACCT GTGCCGCACT CTGCAAACAG GAATGCCAAG
    CCCTTTCTGC AGGCGGTGCG CTCTGTGGAT TGTGAGCAGC ACAGCTATTT TGTCCGTGGC
    ATCTTCTCAA CTCTCTCAAG TGGCTTTTCA AAGATAATAA ATCGAAGGGG TGAGCAGCCC
    CCCACTGAGC CACTGGAGGA GTGGAAACCA GCCGGTAGTC AAATTGACCC GTGCCCAGAT
    GTAAAAGGAC CACCTACGCA CAGGCTGTCC TGCGGCCAGT CCCCCTACAC CGAGACGACA
    ACATGGGAGA GGAAGTACTG CATCCTGACT GACAGCCAGC TGGTGCTGCT CAACAGGGAG
    AAGGAGGTGC CCACCGAGGG GTTGCAGGAG CCGCAGGATG CAACCAAAGG CCGCTGTCTG
    CGCCGCACGG TGAGCGTGCC CTCCGAGGGC CAGTTCCCCG AGTACCCACC AGATGGGGCC
    GCCAAGCTGG AGGTCCCGGC AGAGAGATCT CCTCGCAGAC GGAGCATCTC TGGGACCAGC
    ACATCTGAGA AAACCAACTC CATGGATGCT GCAAATACCT CTCCTTTCAA AGTATCGGGC
    TTCTTCAGTA AACGTCTGAA AGGGTCCATC AAGAGGACAA AGAGTCAGTC AAAGCTGGAC
    AGGAACACAA GTTTCCGCCT CCCATCCCTC CGCAATGCTG ATGACAGGTC ACGTGGGCTG
    CCGAAGCTGA AGGAGTCCCG CTCTCATGAG TCCCTGCTGA GCCCCGGCAG CGCAGTGGAA
    GCCCTGGACC TTGGGGCAGA AGAAAAGGTC TTTGTAAAGC CACTGCACAG CAGCATCCTC
    GGACAGGACT TCTGCTTTGA GGTAACCTAC TCCAGCGGCA GTAAATGCTT CAGCTGTTCC
    TCTGCTGCAG AGAGGGATAA GTGGATGGAA AACCTACGCA GAACAGTGCA GCCGAATAAG
    GACAACTGCC GTCGAGCTGA AAATGTGCTC CGTCTCTGGA TCATTGAGGC AAAGGACCTG
    GCCCCCAAGA AGAAATACTT CTGTGAGCTG TGCCTTGATG ACACTCTCTT TGCCCGCACC
    ACCAGCAAAA CGAAAGCAGA TAACATTTTC TGGGGGGAGC ACTTTGAGTT CTACGGCCTC
    CCAGCTCTTC ACAGCATCAC GGTTCACATC TACAAGGATG TGGAGAAGAA AAAGAAAAAG
    GACAAAAACA ATTACGTCGG CCTGGTCAAC ATTCCCATGG CCACTGTAAC GGGGCGCCAG
    TTTGTGGAAA AATGGTACCC AGTCAGCACA CCTACACCCA ACAAAGGGAA ATCAGGAGGC
    CCTTCCATTC GCATCAAGTC GCGTTATCAG ACCATCACCA TCTTACCCAT GGAGCAATAC
    AAGGAGTTTG CAGAGTTTGT TACCAGCAAC TACACGATGC TGTGCTCTGT GCTGGAGCCA
    GTCATCAGCG TAAGGAATAA GGAAGAGATG GCTTGTGCAA TGGTGCACAT TCTTCAGAGC
    ACGGGGAGAG CCAAGGACTT CCTAACGGAT CTGGTGATGT CTGAGGTGGA TCGCTGCGGG
    GAACACGATG TCTTGATCTT CAGGGAGAAC ACACTTGCTA CCAAAGCTAT TGAGGAATAC
    CTGAAGTTGG TAGGGCAGAA GTATCTCCAT GATGCGCTAG GGGAGTTTAT CAAGGCTTTG
    TATGAGTCAG ATGAAAACTG TGAAGTGGAC CCCAGCAAAT GTTCATCCAG TGAATTAACA
    GACCATCAGA GCAACCTGAA AATGTGTTGT GAGCTGGCCT TCTGCAAGAT TATCAATTCC
    TACTGCGTGT TCCCTCGTGA GCTGAAGGAG GTGTTTGCAT CTTGGAAGCA GCAGTGCCTG
    AGCAGGGGCA AGCAGGACAT CAGCGAGCGG CTGATCAGTG CCTCCCTGTT CCTCCGCTTC
    CTCTGCCCCG CCATCATGTC CCCCAGCCTC TTCAGCCTCA TGCAGGAGTA TCCTGACGAC
    CGGACATCGC GCACGCTCAC ACTCATTGCT AAAGTCATTC AGAATCTCGC CAATTTTGCT
    AAGTTTGGTA ACAAGGAAGA GTACATGGCT TTCATGAACG ACTTTCTGGA ACATGAGTGG
    GGGGGAATGA AGCGATTTCT CCTGGAGATC TCTAATCCAG ATACCATCTC AAACATGCCT
    GGATTTGAGG GCTATATCGA CCTGGGCAGA GAGCTCTCAG TTTTGCATTC CCTCTTATGG
    GAGGTTGTGT CCCAACTTGA TAAGGGTGAA AATTCCTTCC TACAGGCGAC CGTGGCAAAA
    CTGGGACCTC TTCCTCGTAT TCTTGCCGAC ATCACCAAAT CAATGACCAA CCCTACGCCA
    ATACAGCAGC AGCTGAGGCG TTTCACTGAG CACAGCTCTA GTCCAAACGT CAGTGGCAGT
    CTCTCCTCAG GGCTTCAGAA GATATTCGAG GACCCCACTG ATGGTGACTT GCATAAACTG
    AAGTCTCCAA CCCAGGAAAA CATAGATGGC TATTTCAGGG GCAAGACCTT GCTGTTGGTT
    CAGCAGGCGT CCACCCAGAG CATGACTTAC TCAGATAAGG ACGAAAGAGA GAGCATCTTG
    CCCAACGGAC GTAGCATCTC CCTCATGGAC CTGCAGGATC CCCATACCGC TCACAGCGAC
    CACGCTTCCA TGATGCACGA CGTGCCTTTG CGCTTGGCCG GCAGCCAGCT CTCCATCACC
    CAGGTGGCCA ACATCAAGCA GCTGTGGGAG ACCCAGAGCA CGCCCCAAAG CGCTCCCCAG
    GTGAGGAGGC CGCTGCACCC GGCTTTGAAC CAGCAGGGCA GCCTCCAGCC TCTGTCATTC
    CAGAACCCGG TGTACCACCT CAACAACCCA CCTGCGCCGT TGCCCAAGGC CTCCGTGGAC
    TCCAGCCTGG AGAACCTGAG CACCGCCAGC TCCCAGAGCC GAAGCAACAG CGAAGACTTC
    AAGCTCAGCG GGCCCAGCAA CAGCAGCATG GAGGACTTCA CCAAGCGCAG CACGCAGAGC
    GAGGACTTCT CTAGGCGGCA CACGGTCCCG GACAAGCACG TCCCCATCGC GTTGCCCCGC
    CAGAACAGCA GCAGCCAGGC ACAGATCCGC AAAATGGACC AGGCCGGGTT AGGGGCCAGG
    GCCAAAGCGC CGCAGTCGCT GCCGCACAGC GCGTCCCTGC GCAGCACGGG CAGCATGTCG
    GCCGTGTCGG GGGCCGTGGT GACTGAGCCC GTCCAGAACG GCAGCCGCTC CCGGCAGCAG
    TCGTCCTCCT CCAGGGAGAG CCCTGTGCCG AAGGTGAGGG CCATTCAGAG GCAGCAAACG
    CAGCAGGTCC AGTCTCCTGT GGACTCAGCC ACAATGTCAC CAGTGGAAAG GACAGCAGCG
    TGGGTTCTCA ATAACGGGCA GTACGAGGAG GTCGAGGAGG ACACAGAGCA GAACCCAGAT
    GAAGCCAAGC ATGCTGAGAA GTACGAACAA GAGATAGCAA AGCTGAAGGA ACGCTTGAAA
    GTGTCGAGCC GCCGGCTGGA GGAGTACGAG CGGCGGCTCC TGGTGCAGGA GCAGCAAATG
    CAGAAGCTGC TGATGGAGTA CAAGTCCAGG CTGGAGGACA GCGAGGAGAG ACTGCGCAGG
    CAACAGGAGG AGAAGGACAG CCAGATGAAA AGCATTATCA GCAGGCTCAT GGCAGTTGAA
    GAGGAACTGA AGAAAGATCA TGCAGAGATG CAAGCTGTCA TCGATGCAAA GCAGAAAATT
    ATTGATGCAC AGGTCATTAA TGGGAATGAG ATAATTCAAA CAGGAGAAGC GGATCGTGTC
    CCTGGACTCT GCCAACACGC GGCTGATGAG TGCCCTGACG CAGGTGAAGG AGCGCTACAG
    CATGCAAGTC CGCAATGGCA TCTCCCCCAC CAACCCCACC AAGCTTTCCA TCACGGAGAA
    TGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015145998.1
    CDS637..4902
    Translation

    Target ORF information:

    RefSeq Version XM_015290512.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015290512.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAGTGCTT TTAAAAAGTA TTACAGTCTC TCTCCCAGGA CCTTTAGGAG GTCTTTGTCG 
    GAGCAAGATG CTGGCACCGC TGCCTTAAAG GACTATAATT GGTACCACCA AATGAGAAGC
    AAGAAGGGGA GCAAACAAAC AGCTTTGGAC CGAGAGAAAG CACTCGGAGC GTACAGGTGG
    CAAACACATC CCCGTCTTCC CTGGAGGACA AGCAGAAGAG CACACTCTTG CAGCCTCAGA
    GGGGAAGGTA ATGATTCGAG TCTTGTCAAA AGTTCTGCAA GCATGGATGC TACCAGCGCC
    GACGATCTCC ACCAAGACTC AACGGAGATG CTCTTCCAGG CAAGGACCAG ATCACCGCGG
    GTTCCCTTCA ACCGCTCCCT GTCTGAACCT GTGCCGCACT CTGCAAACAG GAATGCCAAG
    CCCTTTCTGC AGGCGGTGCG CTCTGTGGAT TGTGAGCAGC ACAGCTATTT TGTCCGTGGC
    ATCTTCTCAA CTCTCTCAAG TGGCTTTTCA AAGATAATAA ATCGAAGGGG TGAGCAGCCC
    CCCACTGAGC CACTGGAGGA GTGGAAACCA GCCGGTAGTC AAATTGACCC GTGCCCAGAT
    GTAAAAGGAC CACCTACGCA CAGGCTGTCC TGCGGCCAGT CCCCCTACAC CGAGACGACA
    ACATGGGAGA GGAAGTACTG CATCCTGACT GACAGCCAGC TGGTGCTGCT CAACAGGGAG
    AAGGAGGTGC CCACCGAGGG GTTGCAGGAG CCGCAGGATG CAACCAAAGG CCGCTGTCTG
    CGCCGCACGG TGAGCGTGCC CTCCGAGGGC CAGTTCCCCG AGTACCCACC AGATGGGGCC
    GCCAAGCTGG AGGTCCCGGC AGAGAGATCT CCTCGCAGAC GGAGCATCTC TGGGACCAGC
    ACATCTGAGA AAACCAACTC CATGGATGCT GCAAATACCT CTCCTTTCAA AGTATCGGGC
    TTCTTCAGTA AACGTCTGAA AGGGTCCATC AAGAGGACAA AGAGTCAGTC AAAGCTGGAC
    AGGAACACAA GTTTCCGCCT CCCATCCCTC CGCAATGCTG ATGACAGGTC ACGTGGGCTG
    CCGAAGCTGA AGGAGTCCCG CTCTCATGAG TCCCTGCTGA GCCCCGGCAG CGCAGTGGAA
    GCCCTGGACC TTGGGGCAGA AGAAAAGGTC TTTGTAAAGC CACTGCACAG CAGCATCCTC
    GGACAGGACT TCTGCTTTGA GGTAACCTAC TCCAGCGGCA GTAAATGCTT CAGCTGTTCC
    TCTGCTGCAG AGAGGGATAA GTGGATGGAA AACCTACGCA GAACAGTGCA GCCGAATAAG
    GACAACTGCC GTCGAGCTGA AAATGTGCTC CGTCTCTGGA TCATTGAGGC AAAGGACCTG
    GCCCCCAAGA AGAAATACTT CTGTGAGCTG TGCCTTGATG ACACTCTCTT TGCCCGCACC
    ACCAGCAAAA CGAAAGCAGA TAACATTTTC TGGGGGGAGC ACTTTGAGTT CTACGGCCTC
    CCAGCTCTTC ACAGCATCAC GGTTCACATC TACAAGGATG TGGAGAAGAA AAAGAAAAAG
    GACAAAAACA ATTACGTCGG CCTGGTCAAC ATTCCCATGG CCACTGTAAC GGGGCGCCAG
    TTTGTGGAAA AATGGTACCC AGTCAGCACA CCTACACCCA ACAAAGGGAA ATCAGGAGGC
    CCTTCCATTC GCATCAAGTC GCGTTATCAG ACCATCACCA TCTTACCCAT GGAGCAATAC
    AAGGAGTTTG CAGAGTTTGT TACCAGCAAC TACACGATGC TGTGCTCTGT GCTGGAGCCA
    GTCATCAGCG TAAGGAATAA GGAAGAGATG GCTTGTGCAA TGGTGCACAT TCTTCAGAGC
    ACGGGGAGAG CCAAGGACTT CCTAACGGAT CTGGTGATGT CTGAGGTGGA TCGCTGCGGG
    GAACACGATG TCTTGATCTT CAGGGAGAAC ACACTTGCTA CCAAAGCTAT TGAGGAATAC
    CTGAAGTTGG TAGGGCAGAA GTATCTCCAT GATGCGCTAG GGGAGTTTAT CAAGGCTTTG
    TATGAGTCAG ATGAAAACTG TGAAGTGGAC CCCAGCAAAT GTTCATCCAG TGAATTAACA
    GACCATCAGA GCAACCTGAA AATGTGTTGT GAGCTGGCCT TCTGCAAGAT TATCAATTCC
    TACTGCGTGT TCCCTCGTGA GCTGAAGGAG GTGTTTGCAT CTTGGAAGCA GCAGTGCCTG
    AGCAGGGGCA AGCAGGACAT CAGCGAGCGG CTGATCAGTG CCTCCCTGTT CCTCCGCTTC
    CTCTGCCCCG CCATCATGTC CCCCAGCCTC TTCAGCCTCA TGCAGGAGTA TCCTGACGAC
    CGGACATCGC GCACGCTCAC ACTCATTGCT AAAGTCATTC AGAATCTCGC CAATTTTGCT
    AAGTTTGGTA ACAAGGAAGA GTACATGGCT TTCATGAACG ACTTTCTGGA ACATGAGTGG
    GGGGGAATGA AGCGATTTCT CCTGGAGATC TCTAATCCAG ATACCATCTC AAACATGCCT
    GGATTTGAGG GCTATATCGA CCTGGGCAGA GAGCTCTCAG TTTTGCATTC CCTCTTATGG
    GAGGTTGTGT CCCAACTTGA TAAGGGTGAA AATTCCTTCC TACAGGCGAC CGTGGCAAAA
    CTGGGACCTC TTCCTCGTAT TCTTGCCGAC ATCACCAAAT CAATGACCAA CCCTACGCCA
    ATACAGCAGC AGCTGAGGCG TTTCACTGAG CACAGCTCTA GTCCAAACGT CAGTGGCAGT
    CTCTCCTCAG GGCTTCAGAA GATATTCGAG GACCCCACTG ATGGTGACTT GCATAAACTG
    AAGTCTCCAA CCCAGGAAAA CATAGATGGC TATTTCAGGG GCAAGACCTT GCTGTTGGTT
    CAGCAGGCGT CCACCCAGAG CATGACTTAC TCAGATAAGG ACGAAAGAGA GAGCATCTTG
    CCCAACGGAC GTAGCATCTC CCTCATGGAC CTGCAGGATC CCCATACCGC TCACAGCGAC
    CACGCTTCCA TGATGCACGA CGTGCCTTTG CGCTTGGCCG GCAGCCAGCT CTCCATCACC
    CAGGTGGCCA ACATCAAGCA GCTGTGGGAG ACCCAGAGCA CGCCCCAAAG CGCTCCCCAG
    GTGAGGAGGC CGCTGCACCC GGCTTTGAAC CAGCAGGGCA GCCTCCAGCC TCTGTCATTC
    CAGAACCCGG TGTACCACCT CAACAACCCA CCTGCGCCGT TGCCCAAGGC CTCCGTGGAC
    TCCAGCCTGG AGAACCTGAG CACCGCCAGC TCCCAGAGCC GAAGCAACAG CGAAGACTTC
    AAGCTCAGCG GGCCCAGCAA CAGCAGCATG GAGGACTTCA CCAAGCGCAG CACGCAGAGC
    GAGGACTTCT CTAGGCGGCA CACGGTCCCG GACAAGCACG TCCCCATCGC GTTGCCCCGC
    CAGAACAGCA GCAGCCAGGC ACAGATCCGC AAAATGGACC AGGCCGGGTT AGGGGCCAGG
    GCCAAAGCGC CGCAGTCGCT GCCGCACAGC GCGTCCCTGC GCAGCACGGG CAGCATGTCG
    GCCGTGTCGG GGGCCGTGGT GACTGAGCCC GTCCAGAACG GCAGCCGCTC CCGGCAGCAG
    TCGTCCTCCT CCAGGGAGAG CCCTGTGCCG AAGGTGAGGG CCATTCAGAG GCAGCAAACG
    CAGCAGGTCC AGTCTCCTGT GGACTCAGCC ACAATGTCAC CAGTGGAAAG GACAGCAGCG
    TGGGTTCTCA ATAACGGGCA GTACGAGGAG GTCGAGGAGG ACACAGAGCA GAACCCAGAT
    GAAGCCAAGC ATGCTGAGAA GTACGAACAA GAGATAGCAA AGCTGAAGGA ACGCTTGAAA
    GTGTCGAGCC GCCGGCTGGA GGAGTACGAG CGGCGGCTCC TGGTGCAGGA GCAGCAAATG
    CAGAAGCTGC TGATGGAGTA CAAGTCCAGG CTGGAGGACA GCGAGGAGAG ACTGCGCAGG
    CAACAGGAGG AGAAGGACAG CCAGATGAAA AGCATTATCA GCAGGCTCAT GGCAGTTGAA
    GAGGAACTGA AGAAAGATCA TGCAGAGATG CAAGCTGTCA TCGATGCAAA GCAGAAAATT
    ATTGATGCAC AGGTCATTAA TGGGAATGAG ATAATTCAAA CAGGAGAAGC GGATCGTGTC
    CCTGGACTCT GCCAACACGC GGCTGATGAG TGCCCTGACG CAGGTGAAGG AGCGCTACAG
    CATGCAAGTC CGCAATGGCA TCTCCCCCAC CAACCCCACC AAGCTTTCCA TCACGGAGAA
    TGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39047
    Clone ID Related Accession (Same CDS sequence) XM_015290515.1
    Accession Version XM_015290515.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3717bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product ras GTPase-activating protein nGAP isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCAAGCC TGGTGAGGAA GATGCTAGTG CAGACAAAGC CATGGAGCGA TGTAAAAGGA 
    CCACCTACGC ACAGGCTGTC CTGCGGCCAG TCCCCCTACA CCGAGACGAC AACATGGGAG
    AGGAAGTACT GCATCCTGAC TGACAGCCAG CTGGTGCTGC TCAACAGGGA GAAGGAGGTG
    CCCACCGAGG GGTTGCAGGA GCCGCAGGAT GCAACCAAAG GCCGCTGTCT GCGCCGCACG
    GTGAGCGTGC CCTCCGAGGG CCAGTTCCCC GAGTACCCAC CAGATGGGGC CGCCAAGCTG
    GAGGTCCCGG CAGAGAGATC TCCTCGCAGA CGGAGCATCT CTGGGACCAG CACATCTGAG
    AAAACCAACT CCATGGATGC TGCAAATACC TCTCCTTTCA AAGTATCGGG CTTCTTCAGT
    AAACGTCTGA AAGGGTCCAT CAAGAGGACA AAGAGTCAGT CAAAGCTGGA CAGGAACACA
    AGTTTCCGCC TCCCATCCCT CCGCAATGCT GATGACAGGT CACGTGGGCT GCCGAAGCTG
    AAGGAGTCCC GCTCTCATGA GTCCCTGCTG AGCCCCGGCA GCGCAGTGGA AGCCCTGGAC
    CTTGGGGCAG AAGAAAAGGT CTTTGTAAAG CCACTGCACA GCAGCATCCT CGGACAGGAC
    TTCTGCTTTG AGGTAACCTA CTCCAGCGGC AGTAAATGCT TCAGCTGTTC CTCTGCTGCA
    GAGAGGGATA AGTGGATGGA AAACCTACGC AGAACAGTGC AGCCGAATAA GGACAACTGC
    CGTCGAGCTG AAAATGTGCT CCGTCTCTGG ATCATTGAGG CAAAGGACCT GGCCCCCAAG
    AAGAAATACT TCTGTGAGCT GTGCCTTGAT GACACTCTCT TTGCCCGCAC CACCAGCAAA
    ACGAAAGCAG ATAACATTTT CTGGGGGGAG CACTTTGAGT TCTACGGCCT CCCAGCTCTT
    CACAGCATCA CGGTTCACAT CTACAAGGAT GTGGAGAAGA AAAAGAAAAA GGACAAAAAC
    AATTACGTCG GCCTGGTCAA CATTCCCATG GCCACTGTAA CGGGGCGCCA GTTTGTGGAA
    AAATGGTACC CAGTCAGCAC ACCTACACCC AACAAAGGGA AATCAGGAGG CCCTTCCATT
    CGCATCAAGT CGCGTTATCA GACCATCACC ATCTTACCCA TGGAGCAATA CAAGGAGTTT
    GCAGAGTTTG TTACCAGCAA CTACACGATG CTGTGCTCTG TGCTGGAGCC AGTCATCAGC
    GTAAGGAATA AGGAAGAGAT GGCTTGTGCA ATGGTGCACA TTCTTCAGAG CACGGGGAGA
    GCCAAGGACT TCCTAACGGA TCTGGTGATG TCTGAGGTGG ATCGCTGCGG GGAACACGAT
    GTCTTGATCT TCAGGGAGAA CACACTTGCT ACCAAAGCTA TTGAGGAATA CCTGAAGTTG
    GTAGGGCAGA AGTATCTCCA TGATGCGCTA GGGGAGTTTA TCAAGGCTTT GTATGAGTCA
    GATGAAAACT GTGAAGTGGA CCCCAGCAAA TGTTCATCCA GTGAATTAAC AGACCATCAG
    AGCAACCTGA AAATGTGTTG TGAGCTGGCC TTCTGCAAGA TTATCAATTC CTACTGCGTG
    TTCCCTCGTG AGCTGAAGGA GGTGTTTGCA TCTTGGAAGC AGCAGTGCCT GAGCAGGGGC
    AAGCAGGACA TCAGCGAGCG GCTGATCAGT GCCTCCCTGT TCCTCCGCTT CCTCTGCCCC
    GCCATCATGT CCCCCAGCCT CTTCAGCCTC ATGCAGGAGT ATCCTGACGA CCGGACATCG
    CGCACGCTCA CACTCATTGC TAAAGTCATT CAGAATCTCG CCAATTTTGC TAAGTTTGGT
    AACAAGGAAG AGTACATGGC TTTCATGAAC GACTTTCTGG AACATGAGTG GGGGGGAATG
    AAGCGATTTC TCCTGGAGAT CTCTAATCCA GATACCATCT CAAACATGCC TGGATTTGAG
    GGCTATATCG ACCTGGGCAG AGAGCTCTCA GTTTTGCATT CCCTCTTATG GGAGGTTGTG
    TCCCAACTTG ATAAGGGTGA AAATTCCTTC CTACAGGCGA CCGTGGCAAA ACTGGGACCT
    CTTCCTCGTA TTCTTGCCGA CATCACCAAA TCAATGACCA ACCCTACGCC AATACAGCAG
    CAGCTGAGGC GTTTCACTGA GCACAGCTCT AGTCCAAACG TCAGTGGCAG TCTCTCCTCA
    GGGCTTCAGA AGATATTCGA GGACCCCACT GATGGTGACT TGCATAAACT GAAGTCTCCA
    ACCCAGGAAA ACATAGATGG CTATTTCAGG GGCAAGACCT TGCTGTTGGT TCAGCAGGCG
    TCCACCCAGA GCATGACTTA CTCAGATAAG GACGAAAGAG AGAGCATCTT GCCCAACGGA
    CGTAGCATCT CCCTCATGGA CCTGCAGGAT CCCCATACCG CTCACAGCGA CCACGCTTCC
    ATGATGCACG ACGTGCCTTT GCGCTTGGCC GGCAGCCAGC TCTCCATCAC CCAGGTGGCC
    AACATCAAGC AGCTGTGGGA GACCCAGAGC ACGCCCCAAA GCGCTCCCCA GGTGAGGAGG
    CCGCTGCACC CGGCTTTGAA CCAGCAGGGC AGCCTCCAGC CTCTGTCATT CCAGAACCCG
    GTGTACCACC TCAACAACCC ACCTGCGCCG TTGCCCAAGG CCTCCGTGGA CTCCAGCCTG
    GAGAACCTGA GCACCGCCAG CTCCCAGAGC CGAAGCAACA GCGAAGACTT CAAGCTCAGC
    GGGCCCAGCA ACAGCAGCAT GGAGGACTTC ACCAAGCGCA GCACGCAGAG CGAGGACTTC
    TCTAGGCGGC ACACGGTCCC GGACAAGCAC GTCCCCATCG CGTTGCCCCG CCAGAACAGC
    AGCAGCCAGG CACAGATCCG CAAAATGGAC CAGGCCGGGT TAGGGGCCAG GGCCAAAGCG
    CCGCAGTCGC TGCCGCACAG CGCGTCCCTG CGCAGCACGG GCAGCATGTC GGCCGTGTCG
    GGGGCCGTGG TGACTGAGCC CGTCCAGAAC GGCAGCCGCT CCCGGCAGCA GTCGTCCTCC
    TCCAGGGAGA GCCCTGTGCC GAAGGTGAGG GCCATTCAGA GGCAGCAAAC GCAGCAGGTC
    CAGTCTCCTG TGGACTCAGC CACAATGTCA CCAGTGGAAA GGACAGCAGC GTGGGTTCTC
    AATAACGGGC AGTACGAGGA GGTCGAGGAG GACACAGAGC AGAACCCAGA TGAAGCCAAG
    CATGCTGAGA AGTACGAACA AGAGATAGCA AAGCTGAAGG AACGCTTGAA AGTGTCGAGC
    CGCCGGCTGG AGGAGTACGA GCGGCGGCTC CTGGTGCAGG AGCAGCAAAT GCAGAAGCTG
    CTGATGGAGT ACAAGTCCAG GCTGGAGGAC AGCGAGGAGA GACTGCGCAG GCAACAGGAG
    GAGAAGGACA GCCAGATGAA AAGCATTATC AGCAGGCTCA TGGCAGTTGA AGAGGAACTG
    AAGAAAGATC ATGCAGAGAT GCAAGCTGTC ATCGATGCAA AGCAGAAAAT TATTGATGCA
    CAGGTCATTA ATGGGAATGA GATAATTCAA ACAGGAGAAG CGGATCGTGT CCCTGGACTC
    TGCCAACACG CGGCTGATGA GTGCCCTGAC GCAGGTGAAG GAGCGCTACA GCATGCAAGT
    CCGCAATGGC ATCTCCCCCA CCAACCCCAC CAAGCTTTCC ATCACGGAGA ATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015146001.1
    CDS151..3867
    Translation

    Target ORF information:

    RefSeq Version XM_015290515.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015290515.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCAAGCC TGGTGAGGAA GATGCTAGTG CAGACAAAGC CATGGAGCGA TGTAAAAGGA 
    CCACCTACGC ACAGGCTGTC CTGCGGCCAG TCCCCCTACA CCGAGACGAC AACATGGGAG
    AGGAAGTACT GCATCCTGAC TGACAGCCAG CTGGTGCTGC TCAACAGGGA GAAGGAGGTG
    CCCACCGAGG GGTTGCAGGA GCCGCAGGAT GCAACCAAAG GCCGCTGTCT GCGCCGCACG
    GTGAGCGTGC CCTCCGAGGG CCAGTTCCCC GAGTACCCAC CAGATGGGGC CGCCAAGCTG
    GAGGTCCCGG CAGAGAGATC TCCTCGCAGA CGGAGCATCT CTGGGACCAG CACATCTGAG
    AAAACCAACT CCATGGATGC TGCAAATACC TCTCCTTTCA AAGTATCGGG CTTCTTCAGT
    AAACGTCTGA AAGGGTCCAT CAAGAGGACA AAGAGTCAGT CAAAGCTGGA CAGGAACACA
    AGTTTCCGCC TCCCATCCCT CCGCAATGCT GATGACAGGT CACGTGGGCT GCCGAAGCTG
    AAGGAGTCCC GCTCTCATGA GTCCCTGCTG AGCCCCGGCA GCGCAGTGGA AGCCCTGGAC
    CTTGGGGCAG AAGAAAAGGT CTTTGTAAAG CCACTGCACA GCAGCATCCT CGGACAGGAC
    TTCTGCTTTG AGGTAACCTA CTCCAGCGGC AGTAAATGCT TCAGCTGTTC CTCTGCTGCA
    GAGAGGGATA AGTGGATGGA AAACCTACGC AGAACAGTGC AGCCGAATAA GGACAACTGC
    CGTCGAGCTG AAAATGTGCT CCGTCTCTGG ATCATTGAGG CAAAGGACCT GGCCCCCAAG
    AAGAAATACT TCTGTGAGCT GTGCCTTGAT GACACTCTCT TTGCCCGCAC CACCAGCAAA
    ACGAAAGCAG ATAACATTTT CTGGGGGGAG CACTTTGAGT TCTACGGCCT CCCAGCTCTT
    CACAGCATCA CGGTTCACAT CTACAAGGAT GTGGAGAAGA AAAAGAAAAA GGACAAAAAC
    AATTACGTCG GCCTGGTCAA CATTCCCATG GCCACTGTAA CGGGGCGCCA GTTTGTGGAA
    AAATGGTACC CAGTCAGCAC ACCTACACCC AACAAAGGGA AATCAGGAGG CCCTTCCATT
    CGCATCAAGT CGCGTTATCA GACCATCACC ATCTTACCCA TGGAGCAATA CAAGGAGTTT
    GCAGAGTTTG TTACCAGCAA CTACACGATG CTGTGCTCTG TGCTGGAGCC AGTCATCAGC
    GTAAGGAATA AGGAAGAGAT GGCTTGTGCA ATGGTGCACA TTCTTCAGAG CACGGGGAGA
    GCCAAGGACT TCCTAACGGA TCTGGTGATG TCTGAGGTGG ATCGCTGCGG GGAACACGAT
    GTCTTGATCT TCAGGGAGAA CACACTTGCT ACCAAAGCTA TTGAGGAATA CCTGAAGTTG
    GTAGGGCAGA AGTATCTCCA TGATGCGCTA GGGGAGTTTA TCAAGGCTTT GTATGAGTCA
    GATGAAAACT GTGAAGTGGA CCCCAGCAAA TGTTCATCCA GTGAATTAAC AGACCATCAG
    AGCAACCTGA AAATGTGTTG TGAGCTGGCC TTCTGCAAGA TTATCAATTC CTACTGCGTG
    TTCCCTCGTG AGCTGAAGGA GGTGTTTGCA TCTTGGAAGC AGCAGTGCCT GAGCAGGGGC
    AAGCAGGACA TCAGCGAGCG GCTGATCAGT GCCTCCCTGT TCCTCCGCTT CCTCTGCCCC
    GCCATCATGT CCCCCAGCCT CTTCAGCCTC ATGCAGGAGT ATCCTGACGA CCGGACATCG
    CGCACGCTCA CACTCATTGC TAAAGTCATT CAGAATCTCG CCAATTTTGC TAAGTTTGGT
    AACAAGGAAG AGTACATGGC TTTCATGAAC GACTTTCTGG AACATGAGTG GGGGGGAATG
    AAGCGATTTC TCCTGGAGAT CTCTAATCCA GATACCATCT CAAACATGCC TGGATTTGAG
    GGCTATATCG ACCTGGGCAG AGAGCTCTCA GTTTTGCATT CCCTCTTATG GGAGGTTGTG
    TCCCAACTTG ATAAGGGTGA AAATTCCTTC CTACAGGCGA CCGTGGCAAA ACTGGGACCT
    CTTCCTCGTA TTCTTGCCGA CATCACCAAA TCAATGACCA ACCCTACGCC AATACAGCAG
    CAGCTGAGGC GTTTCACTGA GCACAGCTCT AGTCCAAACG TCAGTGGCAG TCTCTCCTCA
    GGGCTTCAGA AGATATTCGA GGACCCCACT GATGGTGACT TGCATAAACT GAAGTCTCCA
    ACCCAGGAAA ACATAGATGG CTATTTCAGG GGCAAGACCT TGCTGTTGGT TCAGCAGGCG
    TCCACCCAGA GCATGACTTA CTCAGATAAG GACGAAAGAG AGAGCATCTT GCCCAACGGA
    CGTAGCATCT CCCTCATGGA CCTGCAGGAT CCCCATACCG CTCACAGCGA CCACGCTTCC
    ATGATGCACG ACGTGCCTTT GCGCTTGGCC GGCAGCCAGC TCTCCATCAC CCAGGTGGCC
    AACATCAAGC AGCTGTGGGA GACCCAGAGC ACGCCCCAAA GCGCTCCCCA GGTGAGGAGG
    CCGCTGCACC CGGCTTTGAA CCAGCAGGGC AGCCTCCAGC CTCTGTCATT CCAGAACCCG
    GTGTACCACC TCAACAACCC ACCTGCGCCG TTGCCCAAGG CCTCCGTGGA CTCCAGCCTG
    GAGAACCTGA GCACCGCCAG CTCCCAGAGC CGAAGCAACA GCGAAGACTT CAAGCTCAGC
    GGGCCCAGCA ACAGCAGCAT GGAGGACTTC ACCAAGCGCA GCACGCAGAG CGAGGACTTC
    TCTAGGCGGC ACACGGTCCC GGACAAGCAC GTCCCCATCG CGTTGCCCCG CCAGAACAGC
    AGCAGCCAGG CACAGATCCG CAAAATGGAC CAGGCCGGGT TAGGGGCCAG GGCCAAAGCG
    CCGCAGTCGC TGCCGCACAG CGCGTCCCTG CGCAGCACGG GCAGCATGTC GGCCGTGTCG
    GGGGCCGTGG TGACTGAGCC CGTCCAGAAC GGCAGCCGCT CCCGGCAGCA GTCGTCCTCC
    TCCAGGGAGA GCCCTGTGCC GAAGGTGAGG GCCATTCAGA GGCAGCAAAC GCAGCAGGTC
    CAGTCTCCTG TGGACTCAGC CACAATGTCA CCAGTGGAAA GGACAGCAGC GTGGGTTCTC
    AATAACGGGC AGTACGAGGA GGTCGAGGAG GACACAGAGC AGAACCCAGA TGAAGCCAAG
    CATGCTGAGA AGTACGAACA AGAGATAGCA AAGCTGAAGG AACGCTTGAA AGTGTCGAGC
    CGCCGGCTGG AGGAGTACGA GCGGCGGCTC CTGGTGCAGG AGCAGCAAAT GCAGAAGCTG
    CTGATGGAGT ACAAGTCCAG GCTGGAGGAC AGCGAGGAGA GACTGCGCAG GCAACAGGAG
    GAGAAGGACA GCCAGATGAA AAGCATTATC AGCAGGCTCA TGGCAGTTGA AGAGGAACTG
    AAGAAAGATC ATGCAGAGAT GCAAGCTGTC ATCGATGCAA AGCAGAAAAT TATTGATGCA
    CAGGTCATTA ATGGGAATGA GATAATTCAA ACAGGAGAAG CGGATCGTGT CCCTGGACTC
    TGCCAACACG CGGCTGATGA GTGCCCTGAC GCAGGTGAAG GAGCGCTACA GCATGCAAGT
    CCGCAATGGC ATCTCCCCCA CCAACCCCAC CAAGCTTTCC ATCACGGAGA ATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39048
    Clone ID Related Accession (Same CDS sequence) XM_015290516.1
    Accession Version XM_015290516.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3498bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product ras GTPase-activating protein nGAP isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGTCTCC TTTCTCCCGG CGATGTGGCC GAGTGGCTGG ATAGCAGCAC AGAAGATGAG 
    AAAAGCCTTC TGTCGTTTCT CGAGGTCCCG GCAGAGAGAT CTCCTCGCAG ACGGAGCATC
    TCTGGGACCA GCACATCTGA GAAAACCAAC TCCATGGATG CTGCAAATAC CTCTCCTTTC
    AAAGTATCGG GCTTCTTCAG TAAACGTCTG AAAGGGTCCA TCAAGAGGAC AAAGAGTCAG
    TCAAAGCTGG ACAGGAACAC AAGTTTCCGC CTCCCATCCC TCCGCAATGC TGATGACAGG
    TCACGTGGGC TGCCGAAGCT GAAGGAGTCC CGCTCTCATG AGTCCCTGCT GAGCCCCGGC
    AGCGCAGTGG AAGCCCTGGA CCTTGGGGCA GAAGAAAAGG TCTTTGTAAA GCCACTGCAC
    AGCAGCATCC TCGGACAGGA CTTCTGCTTT GAGGTAACCT ACTCCAGCGG CAGTAAATGC
    TTCAGCTGTT CCTCTGCTGC AGAGAGGGAT AAGTGGATGG AAAACCTACG CAGAACAGTG
    CAGCCGAATA AGGACAACTG CCGTCGAGCT GAAAATGTGC TCCGTCTCTG GATCATTGAG
    GCAAAGGACC TGGCCCCCAA GAAGAAATAC TTCTGTGAGC TGTGCCTTGA TGACACTCTC
    TTTGCCCGCA CCACCAGCAA AACGAAAGCA GATAACATTT TCTGGGGGGA GCACTTTGAG
    TTCTACGGCC TCCCAGCTCT TCACAGCATC ACGGTTCACA TCTACAAGGA TGTGGAGAAG
    AAAAAGAAAA AGGACAAAAA CAATTACGTC GGCCTGGTCA ACATTCCCAT GGCCACTGTA
    ACGGGGCGCC AGTTTGTGGA AAAATGGTAC CCAGTCAGCA CACCTACACC CAACAAAGGG
    AAATCAGGAG GCCCTTCCAT TCGCATCAAG TCGCGTTATC AGACCATCAC CATCTTACCC
    ATGGAGCAAT ACAAGGAGTT TGCAGAGTTT GTTACCAGCA ACTACACGAT GCTGTGCTCT
    GTGCTGGAGC CAGTCATCAG CGTAAGGAAT AAGGAAGAGA TGGCTTGTGC AATGGTGCAC
    ATTCTTCAGA GCACGGGGAG AGCCAAGGAC TTCCTAACGG ATCTGGTGAT GTCTGAGGTG
    GATCGCTGCG GGGAACACGA TGTCTTGATC TTCAGGGAGA ACACACTTGC TACCAAAGCT
    ATTGAGGAAT ACCTGAAGTT GGTAGGGCAG AAGTATCTCC ATGATGCGCT AGGGGAGTTT
    ATCAAGGCTT TGTATGAGTC AGATGAAAAC TGTGAAGTGG ACCCCAGCAA ATGTTCATCC
    AGTGAATTAA CAGACCATCA GAGCAACCTG AAAATGTGTT GTGAGCTGGC CTTCTGCAAG
    ATTATCAATT CCTACTGCGT GTTCCCTCGT GAGCTGAAGG AGGTGTTTGC ATCTTGGAAG
    CAGCAGTGCC TGAGCAGGGG CAAGCAGGAC ATCAGCGAGC GGCTGATCAG TGCCTCCCTG
    TTCCTCCGCT TCCTCTGCCC CGCCATCATG TCCCCCAGCC TCTTCAGCCT CATGCAGGAG
    TATCCTGACG ACCGGACATC GCGCACGCTC ACACTCATTG CTAAAGTCAT TCAGAATCTC
    GCCAATTTTG CTAAGTTTGG TAACAAGGAA GAGTACATGG CTTTCATGAA CGACTTTCTG
    GAACATGAGT GGGGGGGAAT GAAGCGATTT CTCCTGGAGA TCTCTAATCC AGATACCATC
    TCAAACATGC CTGGATTTGA GGGCTATATC GACCTGGGCA GAGAGCTCTC AGTTTTGCAT
    TCCCTCTTAT GGGAGGTTGT GTCCCAACTT GATAAGGGTG AAAATTCCTT CCTACAGGCG
    ACCGTGGCAA AACTGGGACC TCTTCCTCGT ATTCTTGCCG ACATCACCAA ATCAATGACC
    AACCCTACGC CAATACAGCA GCAGCTGAGG CGTTTCACTG AGCACAGCTC TAGTCCAAAC
    GTCAGTGGCA GTCTCTCCTC AGGGCTTCAG AAGATATTCG AGGACCCCAC TGATGGTGAC
    TTGCATAAAC TGAAGTCTCC AACCCAGGAA AACATAGATG GCTATTTCAG GGGCAAGACC
    TTGCTGTTGG TTCAGCAGGC GTCCACCCAG AGCATGACTT ACTCAGATAA GGACGAAAGA
    GAGAGCATCT TGCCCAACGG ACGTAGCATC TCCCTCATGG ACCTGCAGGA TCCCCATACC
    GCTCACAGCG ACCACGCTTC CATGATGCAC GACGTGCCTT TGCGCTTGGC CGGCAGCCAG
    CTCTCCATCA CCCAGGTGGC CAACATCAAG CAGCTGTGGG AGACCCAGAG CACGCCCCAA
    AGCGCTCCCC AGGTGAGGAG GCCGCTGCAC CCGGCTTTGA ACCAGCAGGG CAGCCTCCAG
    CCTCTGTCAT TCCAGAACCC GGTGTACCAC CTCAACAACC CACCTGCGCC GTTGCCCAAG
    GCCTCCGTGG ACTCCAGCCT GGAGAACCTG AGCACCGCCA GCTCCCAGAG CCGAAGCAAC
    AGCGAAGACT TCAAGCTCAG CGGGCCCAGC AACAGCAGCA TGGAGGACTT CACCAAGCGC
    AGCACGCAGA GCGAGGACTT CTCTAGGCGG CACACGGTCC CGGACAAGCA CGTCCCCATC
    GCGTTGCCCC GCCAGAACAG CAGCAGCCAG GCACAGATCC GCAAAATGGA CCAGGCCGGG
    TTAGGGGCCA GGGCCAAAGC GCCGCAGTCG CTGCCGCACA GCGCGTCCCT GCGCAGCACG
    GGCAGCATGT CGGCCGTGTC GGGGGCCGTG GTGACTGAGC CCGTCCAGAA CGGCAGCCGC
    TCCCGGCAGC AGTCGTCCTC CTCCAGGGAG AGCCCTGTGC CGAAGGTGAG GGCCATTCAG
    AGGCAGCAAA CGCAGCAGGT CCAGTCTCCT GTGGACTCAG CCACAATGTC ACCAGTGGAA
    AGGACAGCAG CGTGGGTTCT CAATAACGGG CAGTACGAGG AGGTCGAGGA GGACACAGAG
    CAGAACCCAG ATGAAGCCAA GCATGCTGAG AAGTACGAAC AAGAGATAGC AAAGCTGAAG
    GAACGCTTGA AAGTGTCGAG CCGCCGGCTG GAGGAGTACG AGCGGCGGCT CCTGGTGCAG
    GAGCAGCAAA TGCAGAAGCT GCTGATGGAG TACAAGTCCA GGCTGGAGGA CAGCGAGGAG
    AGACTGCGCA GGCAACAGGA GGAGAAGGAC AGCCAGATGA AAAGCATTAT CAGCAGGCTC
    ATGGCAGTTG AAGAGGAACT GAAGAAAGAT CATGCAGAGA TGCAAGCTGT CATCGATGCA
    AAGCAGAAAA TTATTGATGC ACAGGTCATT AATGGGAATG AGATAATTCA AACAGGAGAA
    GCGGATCGTG TCCCTGGACT CTGCCAACAC GCGGCTGATG AGTGCCCTGA CGCAGGTGAA
    GGAGCGCTAC AGCATGCAAG TCCGCAATGG CATCTCCCCC ACCAACCCCA CCAAGCTTTC
    CATCACGGAG AATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015146002.1
    CDS16..3513
    Translation

    Target ORF information:

    RefSeq Version XM_015290516.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015290516.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGTCTCC TTTCTCCCGG CGATGTGGCC GAGTGGCTGG ATAGCAGCAC AGAAGATGAG 
    AAAAGCCTTC TGTCGTTTCT CGAGGTCCCG GCAGAGAGAT CTCCTCGCAG ACGGAGCATC
    TCTGGGACCA GCACATCTGA GAAAACCAAC TCCATGGATG CTGCAAATAC CTCTCCTTTC
    AAAGTATCGG GCTTCTTCAG TAAACGTCTG AAAGGGTCCA TCAAGAGGAC AAAGAGTCAG
    TCAAAGCTGG ACAGGAACAC AAGTTTCCGC CTCCCATCCC TCCGCAATGC TGATGACAGG
    TCACGTGGGC TGCCGAAGCT GAAGGAGTCC CGCTCTCATG AGTCCCTGCT GAGCCCCGGC
    AGCGCAGTGG AAGCCCTGGA CCTTGGGGCA GAAGAAAAGG TCTTTGTAAA GCCACTGCAC
    AGCAGCATCC TCGGACAGGA CTTCTGCTTT GAGGTAACCT ACTCCAGCGG CAGTAAATGC
    TTCAGCTGTT CCTCTGCTGC AGAGAGGGAT AAGTGGATGG AAAACCTACG CAGAACAGTG
    CAGCCGAATA AGGACAACTG CCGTCGAGCT GAAAATGTGC TCCGTCTCTG GATCATTGAG
    GCAAAGGACC TGGCCCCCAA GAAGAAATAC TTCTGTGAGC TGTGCCTTGA TGACACTCTC
    TTTGCCCGCA CCACCAGCAA AACGAAAGCA GATAACATTT TCTGGGGGGA GCACTTTGAG
    TTCTACGGCC TCCCAGCTCT TCACAGCATC ACGGTTCACA TCTACAAGGA TGTGGAGAAG
    AAAAAGAAAA AGGACAAAAA CAATTACGTC GGCCTGGTCA ACATTCCCAT GGCCACTGTA
    ACGGGGCGCC AGTTTGTGGA AAAATGGTAC CCAGTCAGCA CACCTACACC CAACAAAGGG
    AAATCAGGAG GCCCTTCCAT TCGCATCAAG TCGCGTTATC AGACCATCAC CATCTTACCC
    ATGGAGCAAT ACAAGGAGTT TGCAGAGTTT GTTACCAGCA ACTACACGAT GCTGTGCTCT
    GTGCTGGAGC CAGTCATCAG CGTAAGGAAT AAGGAAGAGA TGGCTTGTGC AATGGTGCAC
    ATTCTTCAGA GCACGGGGAG AGCCAAGGAC TTCCTAACGG ATCTGGTGAT GTCTGAGGTG
    GATCGCTGCG GGGAACACGA TGTCTTGATC TTCAGGGAGA ACACACTTGC TACCAAAGCT
    ATTGAGGAAT ACCTGAAGTT GGTAGGGCAG AAGTATCTCC ATGATGCGCT AGGGGAGTTT
    ATCAAGGCTT TGTATGAGTC AGATGAAAAC TGTGAAGTGG ACCCCAGCAA ATGTTCATCC
    AGTGAATTAA CAGACCATCA GAGCAACCTG AAAATGTGTT GTGAGCTGGC CTTCTGCAAG
    ATTATCAATT CCTACTGCGT GTTCCCTCGT GAGCTGAAGG AGGTGTTTGC ATCTTGGAAG
    CAGCAGTGCC TGAGCAGGGG CAAGCAGGAC ATCAGCGAGC GGCTGATCAG TGCCTCCCTG
    TTCCTCCGCT TCCTCTGCCC CGCCATCATG TCCCCCAGCC TCTTCAGCCT CATGCAGGAG
    TATCCTGACG ACCGGACATC GCGCACGCTC ACACTCATTG CTAAAGTCAT TCAGAATCTC
    GCCAATTTTG CTAAGTTTGG TAACAAGGAA GAGTACATGG CTTTCATGAA CGACTTTCTG
    GAACATGAGT GGGGGGGAAT GAAGCGATTT CTCCTGGAGA TCTCTAATCC AGATACCATC
    TCAAACATGC CTGGATTTGA GGGCTATATC GACCTGGGCA GAGAGCTCTC AGTTTTGCAT
    TCCCTCTTAT GGGAGGTTGT GTCCCAACTT GATAAGGGTG AAAATTCCTT CCTACAGGCG
    ACCGTGGCAA AACTGGGACC TCTTCCTCGT ATTCTTGCCG ACATCACCAA ATCAATGACC
    AACCCTACGC CAATACAGCA GCAGCTGAGG CGTTTCACTG AGCACAGCTC TAGTCCAAAC
    GTCAGTGGCA GTCTCTCCTC AGGGCTTCAG AAGATATTCG AGGACCCCAC TGATGGTGAC
    TTGCATAAAC TGAAGTCTCC AACCCAGGAA AACATAGATG GCTATTTCAG GGGCAAGACC
    TTGCTGTTGG TTCAGCAGGC GTCCACCCAG AGCATGACTT ACTCAGATAA GGACGAAAGA
    GAGAGCATCT TGCCCAACGG ACGTAGCATC TCCCTCATGG ACCTGCAGGA TCCCCATACC
    GCTCACAGCG ACCACGCTTC CATGATGCAC GACGTGCCTT TGCGCTTGGC CGGCAGCCAG
    CTCTCCATCA CCCAGGTGGC CAACATCAAG CAGCTGTGGG AGACCCAGAG CACGCCCCAA
    AGCGCTCCCC AGGTGAGGAG GCCGCTGCAC CCGGCTTTGA ACCAGCAGGG CAGCCTCCAG
    CCTCTGTCAT TCCAGAACCC GGTGTACCAC CTCAACAACC CACCTGCGCC GTTGCCCAAG
    GCCTCCGTGG ACTCCAGCCT GGAGAACCTG AGCACCGCCA GCTCCCAGAG CCGAAGCAAC
    AGCGAAGACT TCAAGCTCAG CGGGCCCAGC AACAGCAGCA TGGAGGACTT CACCAAGCGC
    AGCACGCAGA GCGAGGACTT CTCTAGGCGG CACACGGTCC CGGACAAGCA CGTCCCCATC
    GCGTTGCCCC GCCAGAACAG CAGCAGCCAG GCACAGATCC GCAAAATGGA CCAGGCCGGG
    TTAGGGGCCA GGGCCAAAGC GCCGCAGTCG CTGCCGCACA GCGCGTCCCT GCGCAGCACG
    GGCAGCATGT CGGCCGTGTC GGGGGCCGTG GTGACTGAGC CCGTCCAGAA CGGCAGCCGC
    TCCCGGCAGC AGTCGTCCTC CTCCAGGGAG AGCCCTGTGC CGAAGGTGAG GGCCATTCAG
    AGGCAGCAAA CGCAGCAGGT CCAGTCTCCT GTGGACTCAG CCACAATGTC ACCAGTGGAA
    AGGACAGCAG CGTGGGTTCT CAATAACGGG CAGTACGAGG AGGTCGAGGA GGACACAGAG
    CAGAACCCAG ATGAAGCCAA GCATGCTGAG AAGTACGAAC AAGAGATAGC AAAGCTGAAG
    GAACGCTTGA AAGTGTCGAG CCGCCGGCTG GAGGAGTACG AGCGGCGGCT CCTGGTGCAG
    GAGCAGCAAA TGCAGAAGCT GCTGATGGAG TACAAGTCCA GGCTGGAGGA CAGCGAGGAG
    AGACTGCGCA GGCAACAGGA GGAGAAGGAC AGCCAGATGA AAAGCATTAT CAGCAGGCTC
    ATGGCAGTTG AAGAGGAACT GAAGAAAGAT CATGCAGAGA TGCAAGCTGT CATCGATGCA
    AAGCAGAAAA TTATTGATGC ACAGGTCATT AATGGGAATG AGATAATTCA AACAGGAGAA
    GCGGATCGTG TCCCTGGACT CTGCCAACAC GCGGCTGATG AGTGCCCTGA CGCAGGTGAA
    GGAGCGCTAC AGCATGCAAG TCCGCAATGG CATCTCCCCC ACCAACCCCA CCAAGCTTTC
    CATCACGGAG AATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39049
    Clone ID Related Accession (Same CDS sequence) XM_004943254.2
    Accession Version XM_004943254.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3438bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product ras GTPase-activating protein nGAP isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004943254.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGGCAGCA CTTGTGACAA AGAGGTCCCG GCAGAGAGAT CTCCTCGCAG ACGGAGCATC 
    TCTGGGACCA GCACATCTGA GAAAACCAAC TCCATGGATG CTGCAAATAC CTCTCCTTTC
    AAAGTATCGG GCTTCTTCAG TAAACGTCTG AAAGGGTCCA TCAAGAGGAC AAAGAGTCAG
    TCAAAGCTGG ACAGGAACAC AAGTTTCCGC CTCCCATCCC TCCGCAATGC TGATGACAGG
    TCACGTGGGC TGCCGAAGCT GAAGGAGTCC CGCTCTCATG AGTCCCTGCT GAGCCCCGGC
    AGCGCAGTGG AAGCCCTGGA CCTTGGGGCA GAAGAAAAGG TCTTTGTAAA GCCACTGCAC
    AGCAGCATCC TCGGACAGGA CTTCTGCTTT GAGGTAACCT ACTCCAGCGG CAGTAAATGC
    TTCAGCTGTT CCTCTGCTGC AGAGAGGGAT AAGTGGATGG AAAACCTACG CAGAACAGTG
    CAGCCGAATA AGGACAACTG CCGTCGAGCT GAAAATGTGC TCCGTCTCTG GATCATTGAG
    GCAAAGGACC TGGCCCCCAA GAAGAAATAC TTCTGTGAGC TGTGCCTTGA TGACACTCTC
    TTTGCCCGCA CCACCAGCAA AACGAAAGCA GATAACATTT TCTGGGGGGA GCACTTTGAG
    TTCTACGGCC TCCCAGCTCT TCACAGCATC ACGGTTCACA TCTACAAGGA TGTGGAGAAG
    AAAAAGAAAA AGGACAAAAA CAATTACGTC GGCCTGGTCA ACATTCCCAT GGCCACTGTA
    ACGGGGCGCC AGTTTGTGGA AAAATGGTAC CCAGTCAGCA CACCTACACC CAACAAAGGG
    AAATCAGGAG GCCCTTCCAT TCGCATCAAG TCGCGTTATC AGACCATCAC CATCTTACCC
    ATGGAGCAAT ACAAGGAGTT TGCAGAGTTT GTTACCAGCA ACTACACGAT GCTGTGCTCT
    GTGCTGGAGC CAGTCATCAG CGTAAGGAAT AAGGAAGAGA TGGCTTGTGC AATGGTGCAC
    ATTCTTCAGA GCACGGGGAG AGCCAAGGAC TTCCTAACGG ATCTGGTGAT GTCTGAGGTG
    GATCGCTGCG GGGAACACGA TGTCTTGATC TTCAGGGAGA ACACACTTGC TACCAAAGCT
    ATTGAGGAAT ACCTGAAGTT GGTAGGGCAG AAGTATCTCC ATGATGCGCT AGGGGAGTTT
    ATCAAGGCTT TGTATGAGTC AGATGAAAAC TGTGAAGTGG ACCCCAGCAA ATGTTCATCC
    AGTGAATTAA CAGACCATCA GAGCAACCTG AAAATGTGTT GTGAGCTGGC CTTCTGCAAG
    ATTATCAATT CCTACTGCGT GTTCCCTCGT GAGCTGAAGG AGGTGTTTGC ATCTTGGAAG
    CAGCAGTGCC TGAGCAGGGG CAAGCAGGAC ATCAGCGAGC GGCTGATCAG TGCCTCCCTG
    TTCCTCCGCT TCCTCTGCCC CGCCATCATG TCCCCCAGCC TCTTCAGCCT CATGCAGGAG
    TATCCTGACG ACCGGACATC GCGCACGCTC ACACTCATTG CTAAAGTCAT TCAGAATCTC
    GCCAATTTTG CTAAGTTTGG TAACAAGGAA GAGTACATGG CTTTCATGAA CGACTTTCTG
    GAACATGAGT GGGGGGGAAT GAAGCGATTT CTCCTGGAGA TCTCTAATCC AGATACCATC
    TCAAACATGC CTGGATTTGA GGGCTATATC GACCTGGGCA GAGAGCTCTC AGTTTTGCAT
    TCCCTCTTAT GGGAGGTTGT GTCCCAACTT GATAAGGGTG AAAATTCCTT CCTACAGGCG
    ACCGTGGCAA AACTGGGACC TCTTCCTCGT ATTCTTGCCG ACATCACCAA ATCAATGACC
    AACCCTACGC CAATACAGCA GCAGCTGAGG CGTTTCACTG AGCACAGCTC TAGTCCAAAC
    GTCAGTGGCA GTCTCTCCTC AGGGCTTCAG AAGATATTCG AGGACCCCAC TGATGGTGAC
    TTGCATAAAC TGAAGTCTCC AACCCAGGAA AACATAGATG GCTATTTCAG GGGCAAGACC
    TTGCTGTTGG TTCAGCAGGC GTCCACCCAG AGCATGACTT ACTCAGATAA GGACGAAAGA
    GAGAGCATCT TGCCCAACGG ACGTAGCATC TCCCTCATGG ACCTGCAGGA TCCCCATACC
    GCTCACAGCG ACCACGCTTC CATGATGCAC GACGTGCCTT TGCGCTTGGC CGGCAGCCAG
    CTCTCCATCA CCCAGGTGGC CAACATCAAG CAGCTGTGGG AGACCCAGAG CACGCCCCAA
    AGCGCTCCCC AGGTGAGGAG GCCGCTGCAC CCGGCTTTGA ACCAGCAGGG CAGCCTCCAG
    CCTCTGTCAT TCCAGAACCC GGTGTACCAC CTCAACAACC CACCTGCGCC GTTGCCCAAG
    GCCTCCGTGG ACTCCAGCCT GGAGAACCTG AGCACCGCCA GCTCCCAGAG CCGAAGCAAC
    AGCGAAGACT TCAAGCTCAG CGGGCCCAGC AACAGCAGCA TGGAGGACTT CACCAAGCGC
    AGCACGCAGA GCGAGGACTT CTCTAGGCGG CACACGGTCC CGGACAAGCA CGTCCCCATC
    GCGTTGCCCC GCCAGAACAG CAGCAGCCAG GCACAGATCC GCAAAATGGA CCAGGCCGGG
    TTAGGGGCCA GGGCCAAAGC GCCGCAGTCG CTGCCGCACA GCGCGTCCCT GCGCAGCACG
    GGCAGCATGT CGGCCGTGTC GGGGGCCGTG GTGACTGAGC CCGTCCAGAA CGGCAGCCGC
    TCCCGGCAGC AGTCGTCCTC CTCCAGGGAG AGCCCTGTGC CGAAGGTGAG GGCCATTCAG
    AGGCAGCAAA CGCAGCAGGT CCAGTCTCCT GTGGACTCAG CCACAATGTC ACCAGTGGAA
    AGGACAGCAG CGTGGGTTCT CAATAACGGG CAGTACGAGG AGGTCGAGGA GGACACAGAG
    CAGAACCCAG ATGAAGCCAA GCATGCTGAG AAGTACGAAC AAGAGATAGC AAAGCTGAAG
    GAACGCTTGA AAGTGTCGAG CCGCCGGCTG GAGGAGTACG AGCGGCGGCT CCTGGTGCAG
    GAGCAGCAAA TGCAGAAGCT GCTGATGGAG TACAAGTCCA GGCTGGAGGA CAGCGAGGAG
    AGACTGCGCA GGCAACAGGA GGAGAAGGAC AGCCAGATGA AAAGCATTAT CAGCAGGCTC
    ATGGCAGTTG AAGAGGAACT GAAGAAAGAT CATGCAGAGA TGCAAGCTGT CATCGATGCA
    AAGCAGAAAA TTATTGATGC ACAGGTCATT AATGGGAATG AGATAATTCA AACAGGAGAA
    GCGGATCGTG TCCCTGGACT CTGCCAACAC GCGGCTGATG AGTGCCCTGA CGCAGGTGAA
    GGAGCGCTAC AGCATGCAAG TCCGCAATGG CATCTCCCCC ACCAACCCCA CCAAGCTTTC
    CATCACGGAG AATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004943311.2
    CDS694..4131
    Translation

    Target ORF information:

    RefSeq Version XM_004943254.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004943254.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGGCAGCA CTTGTGACAA AGAGGTCCCG GCAGAGAGAT CTCCTCGCAG ACGGAGCATC 
    TCTGGGACCA GCACATCTGA GAAAACCAAC TCCATGGATG CTGCAAATAC CTCTCCTTTC
    AAAGTATCGG GCTTCTTCAG TAAACGTCTG AAAGGGTCCA TCAAGAGGAC AAAGAGTCAG
    TCAAAGCTGG ACAGGAACAC AAGTTTCCGC CTCCCATCCC TCCGCAATGC TGATGACAGG
    TCACGTGGGC TGCCGAAGCT GAAGGAGTCC CGCTCTCATG AGTCCCTGCT GAGCCCCGGC
    AGCGCAGTGG AAGCCCTGGA CCTTGGGGCA GAAGAAAAGG TCTTTGTAAA GCCACTGCAC
    AGCAGCATCC TCGGACAGGA CTTCTGCTTT GAGGTAACCT ACTCCAGCGG CAGTAAATGC
    TTCAGCTGTT CCTCTGCTGC AGAGAGGGAT AAGTGGATGG AAAACCTACG CAGAACAGTG
    CAGCCGAATA AGGACAACTG CCGTCGAGCT GAAAATGTGC TCCGTCTCTG GATCATTGAG
    GCAAAGGACC TGGCCCCCAA GAAGAAATAC TTCTGTGAGC TGTGCCTTGA TGACACTCTC
    TTTGCCCGCA CCACCAGCAA AACGAAAGCA GATAACATTT TCTGGGGGGA GCACTTTGAG
    TTCTACGGCC TCCCAGCTCT TCACAGCATC ACGGTTCACA TCTACAAGGA TGTGGAGAAG
    AAAAAGAAAA AGGACAAAAA CAATTACGTC GGCCTGGTCA ACATTCCCAT GGCCACTGTA
    ACGGGGCGCC AGTTTGTGGA AAAATGGTAC CCAGTCAGCA CACCTACACC CAACAAAGGG
    AAATCAGGAG GCCCTTCCAT TCGCATCAAG TCGCGTTATC AGACCATCAC CATCTTACCC
    ATGGAGCAAT ACAAGGAGTT TGCAGAGTTT GTTACCAGCA ACTACACGAT GCTGTGCTCT
    GTGCTGGAGC CAGTCATCAG CGTAAGGAAT AAGGAAGAGA TGGCTTGTGC AATGGTGCAC
    ATTCTTCAGA GCACGGGGAG AGCCAAGGAC TTCCTAACGG ATCTGGTGAT GTCTGAGGTG
    GATCGCTGCG GGGAACACGA TGTCTTGATC TTCAGGGAGA ACACACTTGC TACCAAAGCT
    ATTGAGGAAT ACCTGAAGTT GGTAGGGCAG AAGTATCTCC ATGATGCGCT AGGGGAGTTT
    ATCAAGGCTT TGTATGAGTC AGATGAAAAC TGTGAAGTGG ACCCCAGCAA ATGTTCATCC
    AGTGAATTAA CAGACCATCA GAGCAACCTG AAAATGTGTT GTGAGCTGGC CTTCTGCAAG
    ATTATCAATT CCTACTGCGT GTTCCCTCGT GAGCTGAAGG AGGTGTTTGC ATCTTGGAAG
    CAGCAGTGCC TGAGCAGGGG CAAGCAGGAC ATCAGCGAGC GGCTGATCAG TGCCTCCCTG
    TTCCTCCGCT TCCTCTGCCC CGCCATCATG TCCCCCAGCC TCTTCAGCCT CATGCAGGAG
    TATCCTGACG ACCGGACATC GCGCACGCTC ACACTCATTG CTAAAGTCAT TCAGAATCTC
    GCCAATTTTG CTAAGTTTGG TAACAAGGAA GAGTACATGG CTTTCATGAA CGACTTTCTG
    GAACATGAGT GGGGGGGAAT GAAGCGATTT CTCCTGGAGA TCTCTAATCC AGATACCATC
    TCAAACATGC CTGGATTTGA GGGCTATATC GACCTGGGCA GAGAGCTCTC AGTTTTGCAT
    TCCCTCTTAT GGGAGGTTGT GTCCCAACTT GATAAGGGTG AAAATTCCTT CCTACAGGCG
    ACCGTGGCAA AACTGGGACC TCTTCCTCGT ATTCTTGCCG ACATCACCAA ATCAATGACC
    AACCCTACGC CAATACAGCA GCAGCTGAGG CGTTTCACTG AGCACAGCTC TAGTCCAAAC
    GTCAGTGGCA GTCTCTCCTC AGGGCTTCAG AAGATATTCG AGGACCCCAC TGATGGTGAC
    TTGCATAAAC TGAAGTCTCC AACCCAGGAA AACATAGATG GCTATTTCAG GGGCAAGACC
    TTGCTGTTGG TTCAGCAGGC GTCCACCCAG AGCATGACTT ACTCAGATAA GGACGAAAGA
    GAGAGCATCT TGCCCAACGG ACGTAGCATC TCCCTCATGG ACCTGCAGGA TCCCCATACC
    GCTCACAGCG ACCACGCTTC CATGATGCAC GACGTGCCTT TGCGCTTGGC CGGCAGCCAG
    CTCTCCATCA CCCAGGTGGC CAACATCAAG CAGCTGTGGG AGACCCAGAG CACGCCCCAA
    AGCGCTCCCC AGGTGAGGAG GCCGCTGCAC CCGGCTTTGA ACCAGCAGGG CAGCCTCCAG
    CCTCTGTCAT TCCAGAACCC GGTGTACCAC CTCAACAACC CACCTGCGCC GTTGCCCAAG
    GCCTCCGTGG ACTCCAGCCT GGAGAACCTG AGCACCGCCA GCTCCCAGAG CCGAAGCAAC
    AGCGAAGACT TCAAGCTCAG CGGGCCCAGC AACAGCAGCA TGGAGGACTT CACCAAGCGC
    AGCACGCAGA GCGAGGACTT CTCTAGGCGG CACACGGTCC CGGACAAGCA CGTCCCCATC
    GCGTTGCCCC GCCAGAACAG CAGCAGCCAG GCACAGATCC GCAAAATGGA CCAGGCCGGG
    TTAGGGGCCA GGGCCAAAGC GCCGCAGTCG CTGCCGCACA GCGCGTCCCT GCGCAGCACG
    GGCAGCATGT CGGCCGTGTC GGGGGCCGTG GTGACTGAGC CCGTCCAGAA CGGCAGCCGC
    TCCCGGCAGC AGTCGTCCTC CTCCAGGGAG AGCCCTGTGC CGAAGGTGAG GGCCATTCAG
    AGGCAGCAAA CGCAGCAGGT CCAGTCTCCT GTGGACTCAG CCACAATGTC ACCAGTGGAA
    AGGACAGCAG CGTGGGTTCT CAATAACGGG CAGTACGAGG AGGTCGAGGA GGACACAGAG
    CAGAACCCAG ATGAAGCCAA GCATGCTGAG AAGTACGAAC AAGAGATAGC AAAGCTGAAG
    GAACGCTTGA AAGTGTCGAG CCGCCGGCTG GAGGAGTACG AGCGGCGGCT CCTGGTGCAG
    GAGCAGCAAA TGCAGAAGCT GCTGATGGAG TACAAGTCCA GGCTGGAGGA CAGCGAGGAG
    AGACTGCGCA GGCAACAGGA GGAGAAGGAC AGCCAGATGA AAAGCATTAT CAGCAGGCTC
    ATGGCAGTTG AAGAGGAACT GAAGAAAGAT CATGCAGAGA TGCAAGCTGT CATCGATGCA
    AAGCAGAAAA TTATTGATGC ACAGGTCATT AATGGGAATG AGATAATTCA AACAGGAGAA
    GCGGATCGTG TCCCTGGACT CTGCCAACAC GCGGCTGATG AGTGCCCTGA CGCAGGTGAA
    GGAGCGCTAC AGCATGCAAG TCCGCAATGG CATCTCCCCC ACCAACCCCA CCAAGCTTTC
    CATCACGGAG AATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39050
    Clone ID Related Accession (Same CDS sequence) XM_015290519.1
    Accession Version XM_015290519.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3429bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product ras GTPase-activating protein nGAP isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCAGCTCG CAGAGGTCCC GGCAGAGAGA TCTCCTCGCA GACGGAGCAT CTCTGGGACC 
    AGCACATCTG AGAAAACCAA CTCCATGGAT GCTGCAAATA CCTCTCCTTT CAAAGTATCG
    GGCTTCTTCA GTAAACGTCT GAAAGGGTCC ATCAAGAGGA CAAAGAGTCA GTCAAAGCTG
    GACAGGAACA CAAGTTTCCG CCTCCCATCC CTCCGCAATG CTGATGACAG GTCACGTGGG
    CTGCCGAAGC TGAAGGAGTC CCGCTCTCAT GAGTCCCTGC TGAGCCCCGG CAGCGCAGTG
    GAAGCCCTGG ACCTTGGGGC AGAAGAAAAG GTCTTTGTAA AGCCACTGCA CAGCAGCATC
    CTCGGACAGG ACTTCTGCTT TGAGGTAACC TACTCCAGCG GCAGTAAATG CTTCAGCTGT
    TCCTCTGCTG CAGAGAGGGA TAAGTGGATG GAAAACCTAC GCAGAACAGT GCAGCCGAAT
    AAGGACAACT GCCGTCGAGC TGAAAATGTG CTCCGTCTCT GGATCATTGA GGCAAAGGAC
    CTGGCCCCCA AGAAGAAATA CTTCTGTGAG CTGTGCCTTG ATGACACTCT CTTTGCCCGC
    ACCACCAGCA AAACGAAAGC AGATAACATT TTCTGGGGGG AGCACTTTGA GTTCTACGGC
    CTCCCAGCTC TTCACAGCAT CACGGTTCAC ATCTACAAGG ATGTGGAGAA GAAAAAGAAA
    AAGGACAAAA ACAATTACGT CGGCCTGGTC AACATTCCCA TGGCCACTGT AACGGGGCGC
    CAGTTTGTGG AAAAATGGTA CCCAGTCAGC ACACCTACAC CCAACAAAGG GAAATCAGGA
    GGCCCTTCCA TTCGCATCAA GTCGCGTTAT CAGACCATCA CCATCTTACC CATGGAGCAA
    TACAAGGAGT TTGCAGAGTT TGTTACCAGC AACTACACGA TGCTGTGCTC TGTGCTGGAG
    CCAGTCATCA GCGTAAGGAA TAAGGAAGAG ATGGCTTGTG CAATGGTGCA CATTCTTCAG
    AGCACGGGGA GAGCCAAGGA CTTCCTAACG GATCTGGTGA TGTCTGAGGT GGATCGCTGC
    GGGGAACACG ATGTCTTGAT CTTCAGGGAG AACACACTTG CTACCAAAGC TATTGAGGAA
    TACCTGAAGT TGGTAGGGCA GAAGTATCTC CATGATGCGC TAGGGGAGTT TATCAAGGCT
    TTGTATGAGT CAGATGAAAA CTGTGAAGTG GACCCCAGCA AATGTTCATC CAGTGAATTA
    ACAGACCATC AGAGCAACCT GAAAATGTGT TGTGAGCTGG CCTTCTGCAA GATTATCAAT
    TCCTACTGCG TGTTCCCTCG TGAGCTGAAG GAGGTGTTTG CATCTTGGAA GCAGCAGTGC
    CTGAGCAGGG GCAAGCAGGA CATCAGCGAG CGGCTGATCA GTGCCTCCCT GTTCCTCCGC
    TTCCTCTGCC CCGCCATCAT GTCCCCCAGC CTCTTCAGCC TCATGCAGGA GTATCCTGAC
    GACCGGACAT CGCGCACGCT CACACTCATT GCTAAAGTCA TTCAGAATCT CGCCAATTTT
    GCTAAGTTTG GTAACAAGGA AGAGTACATG GCTTTCATGA ACGACTTTCT GGAACATGAG
    TGGGGGGGAA TGAAGCGATT TCTCCTGGAG ATCTCTAATC CAGATACCAT CTCAAACATG
    CCTGGATTTG AGGGCTATAT CGACCTGGGC AGAGAGCTCT CAGTTTTGCA TTCCCTCTTA
    TGGGAGGTTG TGTCCCAACT TGATAAGGGT GAAAATTCCT TCCTACAGGC GACCGTGGCA
    AAACTGGGAC CTCTTCCTCG TATTCTTGCC GACATCACCA AATCAATGAC CAACCCTACG
    CCAATACAGC AGCAGCTGAG GCGTTTCACT GAGCACAGCT CTAGTCCAAA CGTCAGTGGC
    AGTCTCTCCT CAGGGCTTCA GAAGATATTC GAGGACCCCA CTGATGGTGA CTTGCATAAA
    CTGAAGTCTC CAACCCAGGA AAACATAGAT GGCTATTTCA GGGGCAAGAC CTTGCTGTTG
    GTTCAGCAGG CGTCCACCCA GAGCATGACT TACTCAGATA AGGACGAAAG AGAGAGCATC
    TTGCCCAACG GACGTAGCAT CTCCCTCATG GACCTGCAGG ATCCCCATAC CGCTCACAGC
    GACCACGCTT CCATGATGCA CGACGTGCCT TTGCGCTTGG CCGGCAGCCA GCTCTCCATC
    ACCCAGGTGG CCAACATCAA GCAGCTGTGG GAGACCCAGA GCACGCCCCA AAGCGCTCCC
    CAGGTGAGGA GGCCGCTGCA CCCGGCTTTG AACCAGCAGG GCAGCCTCCA GCCTCTGTCA
    TTCCAGAACC CGGTGTACCA CCTCAACAAC CCACCTGCGC CGTTGCCCAA GGCCTCCGTG
    GACTCCAGCC TGGAGAACCT GAGCACCGCC AGCTCCCAGA GCCGAAGCAA CAGCGAAGAC
    TTCAAGCTCA GCGGGCCCAG CAACAGCAGC ATGGAGGACT TCACCAAGCG CAGCACGCAG
    AGCGAGGACT TCTCTAGGCG GCACACGGTC CCGGACAAGC ACGTCCCCAT CGCGTTGCCC
    CGCCAGAACA GCAGCAGCCA GGCACAGATC CGCAAAATGG ACCAGGCCGG GTTAGGGGCC
    AGGGCCAAAG CGCCGCAGTC GCTGCCGCAC AGCGCGTCCC TGCGCAGCAC GGGCAGCATG
    TCGGCCGTGT CGGGGGCCGT GGTGACTGAG CCCGTCCAGA ACGGCAGCCG CTCCCGGCAG
    CAGTCGTCCT CCTCCAGGGA GAGCCCTGTG CCGAAGGTGA GGGCCATTCA GAGGCAGCAA
    ACGCAGCAGG TCCAGTCTCC TGTGGACTCA GCCACAATGT CACCAGTGGA AAGGACAGCA
    GCGTGGGTTC TCAATAACGG GCAGTACGAG GAGGTCGAGG AGGACACAGA GCAGAACCCA
    GATGAAGCCA AGCATGCTGA GAAGTACGAA CAAGAGATAG CAAAGCTGAA GGAACGCTTG
    AAAGTGTCGA GCCGCCGGCT GGAGGAGTAC GAGCGGCGGC TCCTGGTGCA GGAGCAGCAA
    ATGCAGAAGC TGCTGATGGA GTACAAGTCC AGGCTGGAGG ACAGCGAGGA GAGACTGCGC
    AGGCAACAGG AGGAGAAGGA CAGCCAGATG AAAAGCATTA TCAGCAGGCT CATGGCAGTT
    GAAGAGGAAC TGAAGAAAGA TCATGCAGAG ATGCAAGCTG TCATCGATGC AAAGCAGAAA
    ATTATTGATG CACAGGTCAT TAATGGGAAT GAGATAATTC AAACAGGAGA AGCGGATCGT
    GTCCCTGGAC TCTGCCAACA CGCGGCTGAT GAGTGCCCTG ACGCAGGTGA AGGAGCGCTA
    CAGCATGCAA GTCCGCAATG GCATCTCCCC CACCAACCCC ACCAAGCTTT CCATCACGGA
    GAATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015146005.1
    CDS237..3665
    Translation

    Target ORF information:

    RefSeq Version XM_015290519.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015290519.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCAGCTCG CAGAGGTCCC GGCAGAGAGA TCTCCTCGCA GACGGAGCAT CTCTGGGACC 
    AGCACATCTG AGAAAACCAA CTCCATGGAT GCTGCAAATA CCTCTCCTTT CAAAGTATCG
    GGCTTCTTCA GTAAACGTCT GAAAGGGTCC ATCAAGAGGA CAAAGAGTCA GTCAAAGCTG
    GACAGGAACA CAAGTTTCCG CCTCCCATCC CTCCGCAATG CTGATGACAG GTCACGTGGG
    CTGCCGAAGC TGAAGGAGTC CCGCTCTCAT GAGTCCCTGC TGAGCCCCGG CAGCGCAGTG
    GAAGCCCTGG ACCTTGGGGC AGAAGAAAAG GTCTTTGTAA AGCCACTGCA CAGCAGCATC
    CTCGGACAGG ACTTCTGCTT TGAGGTAACC TACTCCAGCG GCAGTAAATG CTTCAGCTGT
    TCCTCTGCTG CAGAGAGGGA TAAGTGGATG GAAAACCTAC GCAGAACAGT GCAGCCGAAT
    AAGGACAACT GCCGTCGAGC TGAAAATGTG CTCCGTCTCT GGATCATTGA GGCAAAGGAC
    CTGGCCCCCA AGAAGAAATA CTTCTGTGAG CTGTGCCTTG ATGACACTCT CTTTGCCCGC
    ACCACCAGCA AAACGAAAGC AGATAACATT TTCTGGGGGG AGCACTTTGA GTTCTACGGC
    CTCCCAGCTC TTCACAGCAT CACGGTTCAC ATCTACAAGG ATGTGGAGAA GAAAAAGAAA
    AAGGACAAAA ACAATTACGT CGGCCTGGTC AACATTCCCA TGGCCACTGT AACGGGGCGC
    CAGTTTGTGG AAAAATGGTA CCCAGTCAGC ACACCTACAC CCAACAAAGG GAAATCAGGA
    GGCCCTTCCA TTCGCATCAA GTCGCGTTAT CAGACCATCA CCATCTTACC CATGGAGCAA
    TACAAGGAGT TTGCAGAGTT TGTTACCAGC AACTACACGA TGCTGTGCTC TGTGCTGGAG
    CCAGTCATCA GCGTAAGGAA TAAGGAAGAG ATGGCTTGTG CAATGGTGCA CATTCTTCAG
    AGCACGGGGA GAGCCAAGGA CTTCCTAACG GATCTGGTGA TGTCTGAGGT GGATCGCTGC
    GGGGAACACG ATGTCTTGAT CTTCAGGGAG AACACACTTG CTACCAAAGC TATTGAGGAA
    TACCTGAAGT TGGTAGGGCA GAAGTATCTC CATGATGCGC TAGGGGAGTT TATCAAGGCT
    TTGTATGAGT CAGATGAAAA CTGTGAAGTG GACCCCAGCA AATGTTCATC CAGTGAATTA
    ACAGACCATC AGAGCAACCT GAAAATGTGT TGTGAGCTGG CCTTCTGCAA GATTATCAAT
    TCCTACTGCG TGTTCCCTCG TGAGCTGAAG GAGGTGTTTG CATCTTGGAA GCAGCAGTGC
    CTGAGCAGGG GCAAGCAGGA CATCAGCGAG CGGCTGATCA GTGCCTCCCT GTTCCTCCGC
    TTCCTCTGCC CCGCCATCAT GTCCCCCAGC CTCTTCAGCC TCATGCAGGA GTATCCTGAC
    GACCGGACAT CGCGCACGCT CACACTCATT GCTAAAGTCA TTCAGAATCT CGCCAATTTT
    GCTAAGTTTG GTAACAAGGA AGAGTACATG GCTTTCATGA ACGACTTTCT GGAACATGAG
    TGGGGGGGAA TGAAGCGATT TCTCCTGGAG ATCTCTAATC CAGATACCAT CTCAAACATG
    CCTGGATTTG AGGGCTATAT CGACCTGGGC AGAGAGCTCT CAGTTTTGCA TTCCCTCTTA
    TGGGAGGTTG TGTCCCAACT TGATAAGGGT GAAAATTCCT TCCTACAGGC GACCGTGGCA
    AAACTGGGAC CTCTTCCTCG TATTCTTGCC GACATCACCA AATCAATGAC CAACCCTACG
    CCAATACAGC AGCAGCTGAG GCGTTTCACT GAGCACAGCT CTAGTCCAAA CGTCAGTGGC
    AGTCTCTCCT CAGGGCTTCA GAAGATATTC GAGGACCCCA CTGATGGTGA CTTGCATAAA
    CTGAAGTCTC CAACCCAGGA AAACATAGAT GGCTATTTCA GGGGCAAGAC CTTGCTGTTG
    GTTCAGCAGG CGTCCACCCA GAGCATGACT TACTCAGATA AGGACGAAAG AGAGAGCATC
    TTGCCCAACG GACGTAGCAT CTCCCTCATG GACCTGCAGG ATCCCCATAC CGCTCACAGC
    GACCACGCTT CCATGATGCA CGACGTGCCT TTGCGCTTGG CCGGCAGCCA GCTCTCCATC
    ACCCAGGTGG CCAACATCAA GCAGCTGTGG GAGACCCAGA GCACGCCCCA AAGCGCTCCC
    CAGGTGAGGA GGCCGCTGCA CCCGGCTTTG AACCAGCAGG GCAGCCTCCA GCCTCTGTCA
    TTCCAGAACC CGGTGTACCA CCTCAACAAC CCACCTGCGC CGTTGCCCAA GGCCTCCGTG
    GACTCCAGCC TGGAGAACCT GAGCACCGCC AGCTCCCAGA GCCGAAGCAA CAGCGAAGAC
    TTCAAGCTCA GCGGGCCCAG CAACAGCAGC ATGGAGGACT TCACCAAGCG CAGCACGCAG
    AGCGAGGACT TCTCTAGGCG GCACACGGTC CCGGACAAGC ACGTCCCCAT CGCGTTGCCC
    CGCCAGAACA GCAGCAGCCA GGCACAGATC CGCAAAATGG ACCAGGCCGG GTTAGGGGCC
    AGGGCCAAAG CGCCGCAGTC GCTGCCGCAC AGCGCGTCCC TGCGCAGCAC GGGCAGCATG
    TCGGCCGTGT CGGGGGCCGT GGTGACTGAG CCCGTCCAGA ACGGCAGCCG CTCCCGGCAG
    CAGTCGTCCT CCTCCAGGGA GAGCCCTGTG CCGAAGGTGA GGGCCATTCA GAGGCAGCAA
    ACGCAGCAGG TCCAGTCTCC TGTGGACTCA GCCACAATGT CACCAGTGGA AAGGACAGCA
    GCGTGGGTTC TCAATAACGG GCAGTACGAG GAGGTCGAGG AGGACACAGA GCAGAACCCA
    GATGAAGCCA AGCATGCTGA GAAGTACGAA CAAGAGATAG CAAAGCTGAA GGAACGCTTG
    AAAGTGTCGA GCCGCCGGCT GGAGGAGTAC GAGCGGCGGC TCCTGGTGCA GGAGCAGCAA
    ATGCAGAAGC TGCTGATGGA GTACAAGTCC AGGCTGGAGG ACAGCGAGGA GAGACTGCGC
    AGGCAACAGG AGGAGAAGGA CAGCCAGATG AAAAGCATTA TCAGCAGGCT CATGGCAGTT
    GAAGAGGAAC TGAAGAAAGA TCATGCAGAG ATGCAAGCTG TCATCGATGC AAAGCAGAAA
    ATTATTGATG CACAGGTCAT TAATGGGAAT GAGATAATTC AAACAGGAGA AGCGGATCGT
    GTCCCTGGAC TCTGCCAACA CGCGGCTGAT GAGTGCCCTG ACGCAGGTGA AGGAGCGCTA
    CAGCATGCAA GTCCGCAATG GCATCTCCCC CACCAACCCC ACCAAGCTTT CCATCACGGA
    GAATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39051
    Clone ID Related Accession (Same CDS sequence) XM_015290520.1
    Accession Version XM_015290520.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3327bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product ras GTPase-activating protein nGAP isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGGGCCGC CAAGCTGGGG CTTCTTCAGT AAACGTCTGA AAGGGTCCAT CAAGAGGACA 
    AAGAGTCAGT CAAAGCTGGA CAGGAACACA AGTTTCCGCC TCCCATCCCT CCGCAATGCT
    GATGACAGGT CACGTGGGCT GCCGAAGCTG AAGGAGTCCC GCTCTCATGA GTCCCTGCTG
    AGCCCCGGCA GCGCAGTGGA AGCCCTGGAC CTTGGGGCAG AAGAAAAGGT CTTTGTAAAG
    CCACTGCACA GCAGCATCCT CGGACAGGAC TTCTGCTTTG AGGTAACCTA CTCCAGCGGC
    AGTAAATGCT TCAGCTGTTC CTCTGCTGCA GAGAGGGATA AGTGGATGGA AAACCTACGC
    AGAACAGTGC AGCCGAATAA GGACAACTGC CGTCGAGCTG AAAATGTGCT CCGTCTCTGG
    ATCATTGAGG CAAAGGACCT GGCCCCCAAG AAGAAATACT TCTGTGAGCT GTGCCTTGAT
    GACACTCTCT TTGCCCGCAC CACCAGCAAA ACGAAAGCAG ATAACATTTT CTGGGGGGAG
    CACTTTGAGT TCTACGGCCT CCCAGCTCTT CACAGCATCA CGGTTCACAT CTACAAGGAT
    GTGGAGAAGA AAAAGAAAAA GGACAAAAAC AATTACGTCG GCCTGGTCAA CATTCCCATG
    GCCACTGTAA CGGGGCGCCA GTTTGTGGAA AAATGGTACC CAGTCAGCAC ACCTACACCC
    AACAAAGGGA AATCAGGAGG CCCTTCCATT CGCATCAAGT CGCGTTATCA GACCATCACC
    ATCTTACCCA TGGAGCAATA CAAGGAGTTT GCAGAGTTTG TTACCAGCAA CTACACGATG
    CTGTGCTCTG TGCTGGAGCC AGTCATCAGC GTAAGGAATA AGGAAGAGAT GGCTTGTGCA
    ATGGTGCACA TTCTTCAGAG CACGGGGAGA GCCAAGGACT TCCTAACGGA TCTGGTGATG
    TCTGAGGTGG ATCGCTGCGG GGAACACGAT GTCTTGATCT TCAGGGAGAA CACACTTGCT
    ACCAAAGCTA TTGAGGAATA CCTGAAGTTG GTAGGGCAGA AGTATCTCCA TGATGCGCTA
    GGGGAGTTTA TCAAGGCTTT GTATGAGTCA GATGAAAACT GTGAAGTGGA CCCCAGCAAA
    TGTTCATCCA GTGAATTAAC AGACCATCAG AGCAACCTGA AAATGTGTTG TGAGCTGGCC
    TTCTGCAAGA TTATCAATTC CTACTGCGTG TTCCCTCGTG AGCTGAAGGA GGTGTTTGCA
    TCTTGGAAGC AGCAGTGCCT GAGCAGGGGC AAGCAGGACA TCAGCGAGCG GCTGATCAGT
    GCCTCCCTGT TCCTCCGCTT CCTCTGCCCC GCCATCATGT CCCCCAGCCT CTTCAGCCTC
    ATGCAGGAGT ATCCTGACGA CCGGACATCG CGCACGCTCA CACTCATTGC TAAAGTCATT
    CAGAATCTCG CCAATTTTGC TAAGTTTGGT AACAAGGAAG AGTACATGGC TTTCATGAAC
    GACTTTCTGG AACATGAGTG GGGGGGAATG AAGCGATTTC TCCTGGAGAT CTCTAATCCA
    GATACCATCT CAAACATGCC TGGATTTGAG GGCTATATCG ACCTGGGCAG AGAGCTCTCA
    GTTTTGCATT CCCTCTTATG GGAGGTTGTG TCCCAACTTG ATAAGGGTGA AAATTCCTTC
    CTACAGGCGA CCGTGGCAAA ACTGGGACCT CTTCCTCGTA TTCTTGCCGA CATCACCAAA
    TCAATGACCA ACCCTACGCC AATACAGCAG CAGCTGAGGC GTTTCACTGA GCACAGCTCT
    AGTCCAAACG TCAGTGGCAG TCTCTCCTCA GGGCTTCAGA AGATATTCGA GGACCCCACT
    GATGGTGACT TGCATAAACT GAAGTCTCCA ACCCAGGAAA ACATAGATGG CTATTTCAGG
    GGCAAGACCT TGCTGTTGGT TCAGCAGGCG TCCACCCAGA GCATGACTTA CTCAGATAAG
    GACGAAAGAG AGAGCATCTT GCCCAACGGA CGTAGCATCT CCCTCATGGA CCTGCAGGAT
    CCCCATACCG CTCACAGCGA CCACGCTTCC ATGATGCACG ACGTGCCTTT GCGCTTGGCC
    GGCAGCCAGC TCTCCATCAC CCAGGTGGCC AACATCAAGC AGCTGTGGGA GACCCAGAGC
    ACGCCCCAAA GCGCTCCCCA GGTGAGGAGG CCGCTGCACC CGGCTTTGAA CCAGCAGGGC
    AGCCTCCAGC CTCTGTCATT CCAGAACCCG GTGTACCACC TCAACAACCC ACCTGCGCCG
    TTGCCCAAGG CCTCCGTGGA CTCCAGCCTG GAGAACCTGA GCACCGCCAG CTCCCAGAGC
    CGAAGCAACA GCGAAGACTT CAAGCTCAGC GGGCCCAGCA ACAGCAGCAT GGAGGACTTC
    ACCAAGCGCA GCACGCAGAG CGAGGACTTC TCTAGGCGGC ACACGGTCCC GGACAAGCAC
    GTCCCCATCG CGTTGCCCCG CCAGAACAGC AGCAGCCAGG CACAGATCCG CAAAATGGAC
    CAGGCCGGGT TAGGGGCCAG GGCCAAAGCG CCGCAGTCGC TGCCGCACAG CGCGTCCCTG
    CGCAGCACGG GCAGCATGTC GGCCGTGTCG GGGGCCGTGG TGACTGAGCC CGTCCAGAAC
    GGCAGCCGCT CCCGGCAGCA GTCGTCCTCC TCCAGGGAGA GCCCTGTGCC GAAGGTGAGG
    GCCATTCAGA GGCAGCAAAC GCAGCAGGTC CAGTCTCCTG TGGACTCAGC CACAATGTCA
    CCAGTGGAAA GGACAGCAGC GTGGGTTCTC AATAACGGGC AGTACGAGGA GGTCGAGGAG
    GACACAGAGC AGAACCCAGA TGAAGCCAAG CATGCTGAGA AGTACGAACA AGAGATAGCA
    AAGCTGAAGG AACGCTTGAA AGTGTCGAGC CGCCGGCTGG AGGAGTACGA GCGGCGGCTC
    CTGGTGCAGG AGCAGCAAAT GCAGAAGCTG CTGATGGAGT ACAAGTCCAG GCTGGAGGAC
    AGCGAGGAGA GACTGCGCAG GCAACAGGAG GAGAAGGACA GCCAGATGAA AAGCATTATC
    AGCAGGCTCA TGGCAGTTGA AGAGGAACTG AAGAAAGATC ATGCAGAGAT GCAAGCTGTC
    ATCGATGCAA AGCAGAAAAT TATTGATGCA CAGGTCATTA ATGGGAATGA GATAATTCAA
    ACAGGAGAAG CGGATCGTGT CCCTGGACTC TGCCAACACG CGGCTGATGA GTGCCCTGAC
    GCAGGTGAAG GAGCGCTACA GCATGCAAGT CCGCAATGGC ATCTCCCCCA CCAACCCCAC
    CAAGCTTTCC ATCACGGAGA ATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015146006.1
    CDS88..3414
    Translation

    Target ORF information:

    RefSeq Version XM_015290520.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015290520.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGGGCCGC CAAGCTGGGG CTTCTTCAGT AAACGTCTGA AAGGGTCCAT CAAGAGGACA 
    AAGAGTCAGT CAAAGCTGGA CAGGAACACA AGTTTCCGCC TCCCATCCCT CCGCAATGCT
    GATGACAGGT CACGTGGGCT GCCGAAGCTG AAGGAGTCCC GCTCTCATGA GTCCCTGCTG
    AGCCCCGGCA GCGCAGTGGA AGCCCTGGAC CTTGGGGCAG AAGAAAAGGT CTTTGTAAAG
    CCACTGCACA GCAGCATCCT CGGACAGGAC TTCTGCTTTG AGGTAACCTA CTCCAGCGGC
    AGTAAATGCT TCAGCTGTTC CTCTGCTGCA GAGAGGGATA AGTGGATGGA AAACCTACGC
    AGAACAGTGC AGCCGAATAA GGACAACTGC CGTCGAGCTG AAAATGTGCT CCGTCTCTGG
    ATCATTGAGG CAAAGGACCT GGCCCCCAAG AAGAAATACT TCTGTGAGCT GTGCCTTGAT
    GACACTCTCT TTGCCCGCAC CACCAGCAAA ACGAAAGCAG ATAACATTTT CTGGGGGGAG
    CACTTTGAGT TCTACGGCCT CCCAGCTCTT CACAGCATCA CGGTTCACAT CTACAAGGAT
    GTGGAGAAGA AAAAGAAAAA GGACAAAAAC AATTACGTCG GCCTGGTCAA CATTCCCATG
    GCCACTGTAA CGGGGCGCCA GTTTGTGGAA AAATGGTACC CAGTCAGCAC ACCTACACCC
    AACAAAGGGA AATCAGGAGG CCCTTCCATT CGCATCAAGT CGCGTTATCA GACCATCACC
    ATCTTACCCA TGGAGCAATA CAAGGAGTTT GCAGAGTTTG TTACCAGCAA CTACACGATG
    CTGTGCTCTG TGCTGGAGCC AGTCATCAGC GTAAGGAATA AGGAAGAGAT GGCTTGTGCA
    ATGGTGCACA TTCTTCAGAG CACGGGGAGA GCCAAGGACT TCCTAACGGA TCTGGTGATG
    TCTGAGGTGG ATCGCTGCGG GGAACACGAT GTCTTGATCT TCAGGGAGAA CACACTTGCT
    ACCAAAGCTA TTGAGGAATA CCTGAAGTTG GTAGGGCAGA AGTATCTCCA TGATGCGCTA
    GGGGAGTTTA TCAAGGCTTT GTATGAGTCA GATGAAAACT GTGAAGTGGA CCCCAGCAAA
    TGTTCATCCA GTGAATTAAC AGACCATCAG AGCAACCTGA AAATGTGTTG TGAGCTGGCC
    TTCTGCAAGA TTATCAATTC CTACTGCGTG TTCCCTCGTG AGCTGAAGGA GGTGTTTGCA
    TCTTGGAAGC AGCAGTGCCT GAGCAGGGGC AAGCAGGACA TCAGCGAGCG GCTGATCAGT
    GCCTCCCTGT TCCTCCGCTT CCTCTGCCCC GCCATCATGT CCCCCAGCCT CTTCAGCCTC
    ATGCAGGAGT ATCCTGACGA CCGGACATCG CGCACGCTCA CACTCATTGC TAAAGTCATT
    CAGAATCTCG CCAATTTTGC TAAGTTTGGT AACAAGGAAG AGTACATGGC TTTCATGAAC
    GACTTTCTGG AACATGAGTG GGGGGGAATG AAGCGATTTC TCCTGGAGAT CTCTAATCCA
    GATACCATCT CAAACATGCC TGGATTTGAG GGCTATATCG ACCTGGGCAG AGAGCTCTCA
    GTTTTGCATT CCCTCTTATG GGAGGTTGTG TCCCAACTTG ATAAGGGTGA AAATTCCTTC
    CTACAGGCGA CCGTGGCAAA ACTGGGACCT CTTCCTCGTA TTCTTGCCGA CATCACCAAA
    TCAATGACCA ACCCTACGCC AATACAGCAG CAGCTGAGGC GTTTCACTGA GCACAGCTCT
    AGTCCAAACG TCAGTGGCAG TCTCTCCTCA GGGCTTCAGA AGATATTCGA GGACCCCACT
    GATGGTGACT TGCATAAACT GAAGTCTCCA ACCCAGGAAA ACATAGATGG CTATTTCAGG
    GGCAAGACCT TGCTGTTGGT TCAGCAGGCG TCCACCCAGA GCATGACTTA CTCAGATAAG
    GACGAAAGAG AGAGCATCTT GCCCAACGGA CGTAGCATCT CCCTCATGGA CCTGCAGGAT
    CCCCATACCG CTCACAGCGA CCACGCTTCC ATGATGCACG ACGTGCCTTT GCGCTTGGCC
    GGCAGCCAGC TCTCCATCAC CCAGGTGGCC AACATCAAGC AGCTGTGGGA GACCCAGAGC
    ACGCCCCAAA GCGCTCCCCA GGTGAGGAGG CCGCTGCACC CGGCTTTGAA CCAGCAGGGC
    AGCCTCCAGC CTCTGTCATT CCAGAACCCG GTGTACCACC TCAACAACCC ACCTGCGCCG
    TTGCCCAAGG CCTCCGTGGA CTCCAGCCTG GAGAACCTGA GCACCGCCAG CTCCCAGAGC
    CGAAGCAACA GCGAAGACTT CAAGCTCAGC GGGCCCAGCA ACAGCAGCAT GGAGGACTTC
    ACCAAGCGCA GCACGCAGAG CGAGGACTTC TCTAGGCGGC ACACGGTCCC GGACAAGCAC
    GTCCCCATCG CGTTGCCCCG CCAGAACAGC AGCAGCCAGG CACAGATCCG CAAAATGGAC
    CAGGCCGGGT TAGGGGCCAG GGCCAAAGCG CCGCAGTCGC TGCCGCACAG CGCGTCCCTG
    CGCAGCACGG GCAGCATGTC GGCCGTGTCG GGGGCCGTGG TGACTGAGCC CGTCCAGAAC
    GGCAGCCGCT CCCGGCAGCA GTCGTCCTCC TCCAGGGAGA GCCCTGTGCC GAAGGTGAGG
    GCCATTCAGA GGCAGCAAAC GCAGCAGGTC CAGTCTCCTG TGGACTCAGC CACAATGTCA
    CCAGTGGAAA GGACAGCAGC GTGGGTTCTC AATAACGGGC AGTACGAGGA GGTCGAGGAG
    GACACAGAGC AGAACCCAGA TGAAGCCAAG CATGCTGAGA AGTACGAACA AGAGATAGCA
    AAGCTGAAGG AACGCTTGAA AGTGTCGAGC CGCCGGCTGG AGGAGTACGA GCGGCGGCTC
    CTGGTGCAGG AGCAGCAAAT GCAGAAGCTG CTGATGGAGT ACAAGTCCAG GCTGGAGGAC
    AGCGAGGAGA GACTGCGCAG GCAACAGGAG GAGAAGGACA GCCAGATGAA AAGCATTATC
    AGCAGGCTCA TGGCAGTTGA AGAGGAACTG AAGAAAGATC ATGCAGAGAT GCAAGCTGTC
    ATCGATGCAA AGCAGAAAAT TATTGATGCA CAGGTCATTA ATGGGAATGA GATAATTCAA
    ACAGGAGAAG CGGATCGTGT CCCTGGACTC TGCCAACACG CGGCTGATGA GTGCCCTGAC
    GCAGGTGAAG GAGCGCTACA GCATGCAAGT CCGCAATGGC ATCTCCCCCA CCAACCCCAC
    CAAGCTTTCC ATCACGGAGA ATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa11742
    Clone ID Related Accession (Same CDS sequence) XM_004943250.3
    Accession Version XM_004943250.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3819bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product ras GTPase-activating protein nGAP isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006095.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004943250.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGGCGG CCCCCGCGGC GAGCGAGCCG GCGGCCTGGG CGGCCGAGGC GCTGGCGGAG 
    CCGGAGCTGG GCTCGGACGA GCTGGAGGCG GCGGGCGGAG CGCTGGACCG CGTCCTGCTG
    GACTCGGTGT GCCAGCAGCA GGGCTGGGTC CGCGTCTACG ATGTAAAAGG ACCACCTACG
    CACAGGCTGT CCTGCGGCCA GTCCCCCTAC ACCGAGACGA CAACATGGGA GAGGAAGTAC
    TGCATCCTGA CTGACAGCCA GCTGGTGCTG CTCAACAGGG AGAAGGAGGT GCCCACCGAG
    GGGTTGCAGG AGCCGCAGGA TGCAACCAAA GGCCGCTGTC TGCGCCGCAC GGTGAGCGTG
    CCCTCCGAGG GCCAGTTCCC CGAGTACCCA CCAGATGGGG CCGCCAAGCT GGAGGTCCCG
    GCAGAGAGAT CTCCTCGCAG ACGGAGCATC TCTGGGACCA GCACATCTGA GAAAACCAAC
    TCCATGGATG CTGCAAATAC CTCTCCTTTC AAAGTATCGG GCTTCTTCAG TAAACGTCTG
    AAAGGGTCCA TCAAGAGGAC AAAGAGTCAG TCAAAGCTGG ACAGGAACAC AAGTTTCCGC
    CTCCCATCCC TCCGCAATGC TGATGACAGG TCACGTGGGC TGCCGAAGCT GAAGGAGTCC
    CGCTCTCATG AGTCCCTGCT GAGCCCCGGC AGCGCAGTGG AAGCCCTGGA CCTTGGGGCA
    GAAGAAAAGG TCTTTGTAAA GCCACTGCAC AGCAGCATCC TCGGACAGGA CTTCTGCTTT
    GAGGTAACCT ACTCCAGCGG CAGTAAATGC TTCAGCTGTT CCTCTGCTGC AGAGAGGGAT
    AAGTGGATGG AAAACCTACG CAGAACAGTG CAGCCGAATA AGGACAACTG CCGTCGAGCT
    GAAAATGTGC TCCGTCTCTG GATCATTGAG GCAAAGGACC TGGCCCCCAA GAAGAAATAC
    TTCTGTGAGC TGTGCCTTGA TGACACTCTC TTTGCCCGCA CCACCAGCAA AACGAAAGCA
    GATAACATTT TCTGGGGGGA GCACTTTGAG TTCTACGGCC TCCCAGCTCT TCACAGCATC
    ACGGTTCACA TCTACAAGGA TGTGGAGAAG AAAAAGAAAA AGGACAAAAA CAATTACGTC
    GGCCTGGTCA ACATTCCCAT GGCCACTGTA ACGGGGCGCC AGTTTGTGGA AAAATGGTAC
    CCAGTCAGCA CACCTACACC CAACAAAGGG AAATCAGGAG GCCCTTCCAT TCGCATCAAG
    TCGCGTTATC AGACCATCAC CATCTTACCC ATGGAGCAAT ACAAGGAGTT TGCAGAGTTT
    GTTACCAGCA ACTACACGAT GCTGTGCTCT GTGCTGGAGC CAGTCATCAG CGTAAGGAAT
    AAGGAAGAGA TGGCTTGTGC AATGGTGCAC ATTCTTCAGA GCACGGGGAG AGCCAAGGAC
    TTCCTAACGG ATCTGGTGAT GTCTGAGGTG GATCGCTGCG GGGAACACGA TGTCTTGATC
    TTCAGGGAGA ACACACTTGC TACCAAAGCT ATTGAGGAAT ACCTGAAGTT GGTAGGGCAG
    AAGTATCTCC ATGATGCGCT AGGGGAGTTT ATCAAGGCTT TGTATGAGTC AGATGAAAAC
    TGTGAAGTGG ACCCCAGCAA ATGTTCATCC AGTGAATTAA CAGACCATCA GAGCAACCTG
    AAAATGTGTT GTGAGCTGGC CTTCTGCAAG ATTATCAATT CCTACTGCGT GTTCCCTCGT
    GAGCTGAAGG AGGTGTTTGC ATCTTGGAAG CAGCAGTGCC TGAGCAGGGG CAAGCAGGAC
    ATCAGCGAGC GGCTGATCAG TGCCTCCCTG TTCCTCCGCT TCCTGTGCCC CGCCATCATG
    TCCCCCAGCC TCTTCAGCCT CATGCAGGAG TATCCTGACG ACCGGACATC GCGCACGCTC
    ACACTCATTG CTAAAGTCAT TCAGAATCTC GCCAATTTTG CTAAGTTTGG TAACAAGGAA
    GAGTACATGG CTTTCATGAA CGACTTTCTG GAACATGAGT GGGGGGGAAT GAAGCGATTT
    CTCCTGGAGA TCTCTAATCC AGATACCATC TCAAACATGC CTGGATTTGA GGGCTATATC
    GACCTGGGCA GAGAGCTCTC AGTTTTGCAT TCCCTCTTAT GGGAGGTTGT GTCCCAACTT
    GATAAGGGTG AAAATTCCTT CCTACAGGCG ACCGTGGCAA AACTGGGACC TCTTCCTCGT
    ATTCTTGCCG ACATCACCAA ATCAATGACC AACCCTACGC CAATACAGCA GCAGCTGAGG
    CGTTTCACTG AGCACAGCTC TAGTCCAAAC GTCAGTGGCA GTCTCTCCTC AGGGCTTCAG
    AAGATATTCG AGGACCCCAC TGATGGTGAC TTGCATAAAC TGAAGTCTCC AACCCAGGAA
    AACATAGATG GCTATTTCAG GGGCAAGACC TTGCTGTTGG TTCAGCAGGC GTCCACCCAG
    AGCATGACTT ACTCAGATAA GGACGAAAGA GAGAGCATCT TGCCCAACGG ACGTAGCATC
    TCCCTCATGG ACCTGCAGGA TCCCCATACC GCTCACAGCG ACCACGCTTC CATGATGCAC
    GACGTGCCTT TGCGCTTGGC CGGCAGCCAG CTCTCCATCA CCCAGGTGGC CAACATCAAG
    CAGCTGTGGG AGACCCAGAG CACGCCCCAA AGCGCTCCCC AGGTGAGGAG GCCGCTGCAC
    CCGGCTTTGA ACCAGCAGGG CAGCCTCCAG CCTCTGTCAT TCCAGAACCC GGTGTACCAC
    CTCAACAACC CACCTGCGCC GTTGCCCAAG GCCTCCGTGG ACTCCAGCCT GGAGAACCTG
    AGCACCGCCA GCTCCCAGAG CCGAAGCAAC AGCGAAGACT TCAAGCTCAG CGGGCCCAGC
    AACAGCAGCA TGGAGGACTT CACCAAGCGC AGCACGCAGA GCGAGGACTT CTCTAGGCGG
    CACACGGTCC CGGACAAGCA CGTCCCCATC GCGTTGCCCC GCCAGAACAG CAGCAGCCAG
    GCACAGATCC GCAAAATGGA CCAGGCCGGG TTAGGGGCCA GGGCCAAAGC GCCGCAGTCG
    CTGCCGCACA GCGCGTCCCT GCGCAGCACG GGCAGCATGT CGGCCGTGTC GGGGGCCGTG
    GTGACTGAGC CCGTCCAGAA CGGCAGCCGC TCCCGGCAGC AGTCGTCCTC CTCCAGGGAG
    AGCCCTGTGC CGAAGGTGAG GGCCATTCAG AGGCAGCAAA CGCAGCAGGT CCAGTCTCCT
    GTGGACTCAG CCACAATGTC ACCAGTGGAA AGGACAGCAG CGTGGGTTCT CAATAACGGG
    CAGTACGAGG AGGTCGAGGA GGACACAGAG CAGAACCCAG ATGAAGCCAA GCATGCTGAG
    AAGTACGAAC AAGAGATAGC AAAGCTGAAG GAACGCTTGA AAGTGTCGAG CCGCCGGCTG
    GAGGAGTACG AGCGGCGGCT CCTGGTGCAG GAGCAGCAAA TGCAGAAGCT GCTGATGGAG
    TACAAGTCCA GGCTGGAGGA CAGCGAGGAG AGACTGCGCA GGCAACAGGA GGAGAAGGAC
    AGCCAGATGA AAAGCATTAT CAGCAGGCTC ATGGCAGTTG AAGAGGAACT GAAGAAAGAT
    CATGCAGAGA TGCAAGCTGT CATCGATGCA AAGCAGAAAA TTATTGATGC ACAGGAGAAG
    CGGATCGTGT CCCTGGACTC TGCCAACACG CGGCTGATGA GTGCCCTGAC GCAGGTGAAG
    GAGCGCTACA GCATGCAAGT CCGCAATGGC ATCTCCCCCA CCAACCCCAC CAAGCTTTCC
    ATCACGGAGA ATGGTGAATT CAAAAACAGC AGCTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004943307.1
    CDS74..3892
    Translation

    Target ORF information:

    RefSeq Version XM_004943250.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004943250.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGGCGG CCCCCGCGGC GAGCGAGCCG GCGGCCTGGG CGGCCGAGGC GCTGGCGGAG 
    CCGGAGCTGG GCTCGGACGA GCTGGAGGCG GCGGGCGGAG CGCTGGACCG CGTCCTGCTG
    GACTCGGTGT GCCAGCAGCA GGGCTGGGTC CGCGTCTACG ATGTAAAAGG ACCACCTACG
    CACAGGCTGT CCTGCGGCCA GTCCCCCTAC ACCGAGACGA CAACATGGGA GAGGAAGTAC
    TGCATCCTGA CTGACAGCCA GCTGGTGCTG CTCAACAGGG AGAAGGAGGT GCCCACCGAG
    GGGTTGCAGG AGCCGCAGGA TGCAACCAAA GGCCGCTGTC TGCGCCGCAC GGTGAGCGTG
    CCCTCCGAGG GCCAGTTCCC CGAGTACCCA CCAGATGGGG CCGCCAAGCT GGAGGTCCCG
    GCAGAGAGAT CTCCTCGCAG ACGGAGCATC TCTGGGACCA GCACATCTGA GAAAACCAAC
    TCCATGGATG CTGCAAATAC CTCTCCTTTC AAAGTATCGG GCTTCTTCAG TAAACGTCTG
    AAAGGGTCCA TCAAGAGGAC AAAGAGTCAG TCAAAGCTGG ACAGGAACAC AAGTTTCCGC
    CTCCCATCCC TCCGCAATGC TGATGACAGG TCACGTGGGC TGCCGAAGCT GAAGGAGTCC
    CGCTCTCATG AGTCCCTGCT GAGCCCCGGC AGCGCAGTGG AAGCCCTGGA CCTTGGGGCA
    GAAGAAAAGG TCTTTGTAAA GCCACTGCAC AGCAGCATCC TCGGACAGGA CTTCTGCTTT
    GAGGTAACCT ACTCCAGCGG CAGTAAATGC TTCAGCTGTT CCTCTGCTGC AGAGAGGGAT
    AAGTGGATGG AAAACCTACG CAGAACAGTG CAGCCGAATA AGGACAACTG CCGTCGAGCT
    GAAAATGTGC TCCGTCTCTG GATCATTGAG GCAAAGGACC TGGCCCCCAA GAAGAAATAC
    TTCTGTGAGC TGTGCCTTGA TGACACTCTC TTTGCCCGCA CCACCAGCAA AACGAAAGCA
    GATAACATTT TCTGGGGGGA GCACTTTGAG TTCTACGGCC TCCCAGCTCT TCACAGCATC
    ACGGTTCACA TCTACAAGGA TGTGGAGAAG AAAAAGAAAA AGGACAAAAA CAATTACGTC
    GGCCTGGTCA ACATTCCCAT GGCCACTGTA ACGGGGCGCC AGTTTGTGGA AAAATGGTAC
    CCAGTCAGCA CACCTACACC CAACAAAGGG AAATCAGGAG GCCCTTCCAT TCGCATCAAG
    TCGCGTTATC AGACCATCAC CATCTTACCC ATGGAGCAAT ACAAGGAGTT TGCAGAGTTT
    GTTACCAGCA ACTACACGAT GCTGTGCTCT GTGCTGGAGC CAGTCATCAG CGTAAGGAAT
    AAGGAAGAGA TGGCTTGTGC AATGGTGCAC ATTCTTCAGA GCACGGGGAG AGCCAAGGAC
    TTCCTAACGG ATCTGGTGAT GTCTGAGGTG GATCGCTGCG GGGAACACGA TGTCTTGATC
    TTCAGGGAGA ACACACTTGC TACCAAAGCT ATTGAGGAAT ACCTGAAGTT GGTAGGGCAG
    AAGTATCTCC ATGATGCGCT AGGGGAGTTT ATCAAGGCTT TGTATGAGTC AGATGAAAAC
    TGTGAAGTGG ACCCCAGCAA ATGTTCATCC AGTGAATTAA CAGACCATCA GAGCAACCTG
    AAAATGTGTT GTGAGCTGGC CTTCTGCAAG ATTATCAATT CCTACTGCGT GTTCCCTCGT
    GAGCTGAAGG AGGTGTTTGC ATCTTGGAAG CAGCAGTGCC TGAGCAGGGG CAAGCAGGAC
    ATCAGCGAGC GGCTGATCAG TGCCTCCCTG TTCCTCCGCT TCCTGTGCCC CGCCATCATG
    TCCCCCAGCC TCTTCAGCCT CATGCAGGAG TATCCTGACG ACCGGACATC GCGCACGCTC
    ACACTCATTG CTAAAGTCAT TCAGAATCTC GCCAATTTTG CTAAGTTTGG TAACAAGGAA
    GAGTACATGG CTTTCATGAA CGACTTTCTG GAACATGAGT GGGGGGGAAT GAAGCGATTT
    CTCCTGGAGA TCTCTAATCC AGATACCATC TCAAACATGC CTGGATTTGA GGGCTATATC
    GACCTGGGCA GAGAGCTCTC AGTTTTGCAT TCCCTCTTAT GGGAGGTTGT GTCCCAACTT
    GATAAGGGTG AAAATTCCTT CCTACAGGCG ACCGTGGCAA AACTGGGACC TCTTCCTCGT
    ATTCTTGCCG ACATCACCAA ATCAATGACC AACCCTACGC CAATACAGCA GCAGCTGAGG
    CGTTTCACTG AGCACAGCTC TAGTCCAAAC GTCAGTGGCA GTCTCTCCTC AGGGCTTCAG
    AAGATATTCG AGGACCCCAC TGATGGTGAC TTGCATAAAC TGAAGTCTCC AACCCAGGAA
    AACATAGATG GCTATTTCAG GGGCAAGACC TTGCTGTTGG TTCAGCAGGC GTCCACCCAG
    AGCATGACTT ACTCAGATAA GGACGAAAGA GAGAGCATCT TGCCCAACGG ACGTAGCATC
    TCCCTCATGG ACCTGCAGGA TCCCCATACC GCTCACAGCG ACCACGCTTC CATGATGCAC
    GACGTGCCTT TGCGCTTGGC CGGCAGCCAG CTCTCCATCA CCCAGGTGGC CAACATCAAG
    CAGCTGTGGG AGACCCAGAG CACGCCCCAA AGCGCTCCCC AGGTGAGGAG GCCGCTGCAC
    CCGGCTTTGA ACCAGCAGGG CAGCCTCCAG CCTCTGTCAT TCCAGAACCC GGTGTACCAC
    CTCAACAACC CACCTGCGCC GTTGCCCAAG GCCTCCGTGG ACTCCAGCCT GGAGAACCTG
    AGCACCGCCA GCTCCCAGAG CCGAAGCAAC AGCGAAGACT TCAAGCTCAG CGGGCCCAGC
    AACAGCAGCA TGGAGGACTT CACCAAGCGC AGCACGCAGA GCGAGGACTT CTCTAGGCGG
    CACACGGTCC CGGACAAGCA CGTCCCCATC GCGTTGCCCC GCCAGAACAG CAGCAGCCAG
    GCACAGATCC GCAAAATGGA CCAGGCCGGG TTAGGGGCCA GGGCCAAAGC GCCGCAGTCG
    CTGCCGCACA GCGCGTCCCT GCGCAGCACG GGCAGCATGT CGGCCGTGTC GGGGGCCGTG
    GTGACTGAGC CCGTCCAGAA CGGCAGCCGC TCCCGGCAGC AGTCGTCCTC CTCCAGGGAG
    AGCCCTGTGC CGAAGGTGAG GGCCATTCAG AGGCAGCAAA CGCAGCAGGT CCAGTCTCCT
    GTGGACTCAG CCACAATGTC ACCAGTGGAA AGGACAGCAG CGTGGGTTCT CAATAACGGG
    CAGTACGAGG AGGTCGAGGA GGACACAGAG CAGAACCCAG ATGAAGCCAA GCATGCTGAG
    AAGTACGAAC AAGAGATAGC AAAGCTGAAG GAACGCTTGA AAGTGTCGAG CCGCCGGCTG
    GAGGAGTACG AGCGGCGGCT CCTGGTGCAG GAGCAGCAAA TGCAGAAGCT GCTGATGGAG
    TACAAGTCCA GGCTGGAGGA CAGCGAGGAG AGACTGCGCA GGCAACAGGA GGAGAAGGAC
    AGCCAGATGA AAAGCATTAT CAGCAGGCTC ATGGCAGTTG AAGAGGAACT GAAGAAAGAT
    CATGCAGAGA TGCAAGCTGT CATCGATGCA AAGCAGAAAA TTATTGATGC ACAGGAGAAG
    CGGATCGTGT CCCTGGACTC TGCCAACACG CGGCTGATGA GTGCCCTGAC GCAGGTGAAG
    GAGCGCTACA GCATGCAAGT CCGCAATGGC ATCTCCCCCA CCAACCCCAC CAAGCTTTCC
    ATCACGGAGA ATGGTGAATT CAAAAACAGC AGCTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39044
    Clone ID Related Accession (Same CDS sequence) XM_015290513.1
    Accession Version XM_015290513.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3828bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product ras GTPase-activating protein nGAP isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGGCGG CCCCCGCGGC GAGCGAGCCG GCGGCCTGGG CGGCCGAGGC GCTGGCGGAG 
    CCGGAGCTGG GCTCGGACGA GCTGGAGGCG GCGGGCGGAG CGCTGGACCG CGTCCTGCTG
    GACTCGGTGT GCCAGCAGCA GGGCTGGGTC CGCGTCTACG ATGTAAAAGG ACCACCTACG
    CACAGGCTGT CCTGCGGCCA GTCCCCCTAC ACCGAGACGA CAACATGGGA GAGGAAGTAC
    TGCATCCTGA CTGACAGCCA GCTGGTGCTG CTCAACAGGG AGAAGGAGGT GCCCACCGAG
    GGGTTGCAGG AGCCGCAGGA TGCAACCAAA GGCCGCTGTC TGCGCCGCAC GGTGAGCGTG
    CCCTCCGAGG GCCAGTTCCC CGAGTACCCA CCAGATGGGG CCGCCAAGCT GGAGGTCCCG
    GCAGAGAGAT CTCCTCGCAG ACGGAGCATC TCTGGGACCA GCACATCTGA GAAAACCAAC
    TCCATGGATG CTGCAAATAC CTCTCCTTTC AAAGTATCGG GCTTCTTCAG TAAACGTCTG
    AAAGGGTCCA TCAAGAGGAC AAAGAGTCAG TCAAAGCTGG ACAGGAACAC AAGTTTCCGC
    CTCCCATCCC TCCGCAATGC TGATGACAGG TCACGTGGGC TGCCGAAGCT GAAGGAGTCC
    CGCTCTCATG AGTCCCTGCT GAGCCCCGGC AGCGCAGTGG AAGCCCTGGA CCTTGGGGCA
    GAAGAAAAGG TCTTTGTAAA GCCACTGCAC AGCAGCATCC TCGGACAGGA CTTCTGCTTT
    GAGGTAACCT ACTCCAGCGG CAGTAAATGC TTCAGCTGTT CCTCTGCTGC AGAGAGGGAT
    AAGTGGATGG AAAACCTACG CAGAACAGTG CAGCCGAATA AGGACAACTG CCGTCGAGCT
    GAAAATGTGC TCCGTCTCTG GATCATTGAG GCAAAGGACC TGGCCCCCAA GAAGAAATAC
    TTCTGTGAGC TGTGCCTTGA TGACACTCTC TTTGCCCGCA CCACCAGCAA AACGAAAGCA
    GATAACATTT TCTGGGGGGA GCACTTTGAG TTCTACGGCC TCCCAGCTCT TCACAGCATC
    ACGGTTCACA TCTACAAGGA TGTGGAGAAG AAAAAGAAAA AGGACAAAAA CAATTACGTC
    GGCCTGGTCA ACATTCCCAT GGCCACTGTA ACGGGGCGCC AGTTTGTGGA AAAATGGTAC
    CCAGTCAGCA CACCTACACC CAACAAAGGG AAATCAGGAG GCCCTTCCAT TCGCATCAAG
    TCGCGTTATC AGACCATCAC CATCTTACCC ATGGAGCAAT ACAAGGAGTT TGCAGAGTTT
    GTTACCAGCA ACTACACGAT GCTGTGCTCT GTGCTGGAGC CAGTCATCAG CGTAAGGAAT
    AAGGAAGAGA TGGCTTGTGC AATGGTGCAC ATTCTTCAGA GCACGGGGAG AGCCAAGGAC
    TTCCTAACGG ATCTGGTGAT GTCTGAGGTG GATCGCTGCG GGGAACACGA TGTCTTGATC
    TTCAGGGAGA ACACACTTGC TACCAAAGCT ATTGAGGAAT ACCTGAAGTT GGTAGGGCAG
    AAGTATCTCC ATGATGCGCT AGGGGAGTTT ATCAAGGCTT TGTATGAGTC AGATGAAAAC
    TGTGAAGTGG ACCCCAGCAA ATGTTCATCC AGTGAATTAA CAGACCATCA GAGCAACCTG
    AAAATGTGTT GTGAGCTGGC CTTCTGCAAG ATTATCAATT CCTACTGCGT GTTCCCTCGT
    GAGCTGAAGG AGGTGTTTGC ATCTTGGAAG CAGCAGTGCC TGAGCAGGGG CAAGCAGGAC
    ATCAGCGAGC GGCTGATCAG TGCCTCCCTG TTCCTCCGCT TCCTCTGCCC CGCCATCATG
    TCCCCCAGCC TCTTCAGCCT CATGCAGGAG TATCCTGACG ACCGGACATC GCGCACGCTC
    ACACTCATTG CTAAAGTCAT TCAGAATCTC GCCAATTTTG CTAAGTTTGG TAACAAGGAA
    GAGTACATGG CTTTCATGAA CGACTTTCTG GAACATGAGT GGGGGGGAAT GAAGCGATTT
    CTCCTGGAGA TCTCTAATCC AGATACCATC TCAAACATGC CTGGATTTGA GGGCTATATC
    GACCTGGGCA GAGAGCTCTC AGTTTTGCAT TCCCTCTTAT GGGAGGTTGT GTCCCAACTT
    GATAAGGGTG AAAATTCCTT CCTACAGGCG ACCGTGGCAA AACTGGGACC TCTTCCTCGT
    ATTCTTGCCG ACATCACCAA ATCAATGACC AACCCTACGC CAATACAGCA GCAGCTGAGG
    CGTTTCACTG AGCACAGCTC TAGTCCAAAC GTCAGTGGCA GTCTCTCCTC AGGGCTTCAG
    AAGATATTCG AGGACCCCAC TGATGGTGAC TTGCATAAAC TGAAGTCTCC AACCCAGGAA
    AACATAGATG GCTATTTCAG GGGCAAGACC TTGCTGTTGG TTCAGCAGGC GTCCACCCAG
    AGCATGACTT ACTCAGATAA GGACGAAAGA GAGAGCATCT TGCCCAACGG ACGTAGCATC
    TCCCTCATGG ACCTGCAGGA TCCCCATACC GCTCACAGCG ACCACGCTTC CATGATGCAC
    GACGTGCCTT TGCGCTTGGC CGGCAGCCAG CTCTCCATCA CCCAGGTGGC CAACATCAAG
    CAGCTGTGGG AGACCCAGAG CACGCCCCAA AGCGCTCCCC AGGTGAGGAG GCCGCTGCAC
    CCGGCTTTGA ACCAGCAGGG CAGCCTCCAG CCTCTGTCAT TCCAGAACCC GGTGTACCAC
    CTCAACAACC CACCTGCGCC GTTGCCCAAG GCCTCCGTGG ACTCCAGCCT GGAGAACCTG
    AGCACCGCCA GCTCCCAGAG CCGAAGCAAC AGCGAAGACT TCAAGCTCAG CGGGCCCAGC
    AACAGCAGCA TGGAGGACTT CACCAAGCGC AGCACGCAGA GCGAGGACTT CTCTAGGCGG
    CACACGGTCC CGGACAAGCA CGTCCCCATC GCGTTGCCCC GCCAGAACAG CAGCAGCCAG
    GCACAGATCC GCAAAATGGA CCAGGCCGGG TTAGGGGCCA GGGCCAAAGC GCCGCAGTCG
    CTGCCGCACA GCGCGTCCCT GCGCAGCACG GGCAGCATGT CGGCCGTGTC GGGGGCCGTG
    GTGACTGAGC CCGTCCAGAA CGGCAGCCGC TCCCGGCAGC AGTCGTCCTC CTCCAGGGAG
    AGCCCTGTGC CGAAGGTGAG GGCCATTCAG AGGCAGCAAA CGCAGCAGGT CCAGTCTCCT
    GTGGACTCAG CCACAATGTC ACCAGTGGAA AGGACAGCAG CGTGGGTTCT CAATAACGGG
    CAGTACGAGG AGGTCGAGGA GGACACAGAG CAGAACCCAG ATGAAGCCAA GCATGCTGAG
    AAGTACGAAC AAGAGATAGC AAAGCTGAAG GAACGCTTGA AAGTGTCGAG CCGCCGGCTG
    GAGGAGTACG AGCGGCGGCT CCTGGTGCAG GAGCAGCAAA TGCAGAAGCT GCTGATGGAG
    TACAAGTCCA GGCTGGAGGA CAGCGAGGAG AGACTGCGCA GGCAACAGGA GGAGAAGGAC
    AGCCAGATGA AAAGCATTAT CAGCAGGCTC ATGGCAGTTG AAGAGGAACT GAAGAAAGAT
    CATGCAGAGA TGCAAGCTGT CATCGATGCA AAGCAGAAAA TTATTGATGC ACAGGTCATT
    AATGGGAATG AGATAATTCA AACAGGAGAA GCGGATCGTG TCCCTGGACT CTGCCAACAC
    GCGGCTGATG AGTGCCCTGA CGCAGGTGAA GGAGCGCTAC AGCATGCAAG TCCGCAATGG
    CATCTCCCCC ACCAACCCCA CCAAGCTTTC CATCACGGAG AATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015145999.1
    CDS78..3905
    Translation

    Target ORF information:

    RefSeq Version XM_015290513.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015290513.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGGCGG CCCCCGCGGC GAGCGAGCCG GCGGCCTGGG CGGCCGAGGC GCTGGCGGAG 
    CCGGAGCTGG GCTCGGACGA GCTGGAGGCG GCGGGCGGAG CGCTGGACCG CGTCCTGCTG
    GACTCGGTGT GCCAGCAGCA GGGCTGGGTC CGCGTCTACG ATGTAAAAGG ACCACCTACG
    CACAGGCTGT CCTGCGGCCA GTCCCCCTAC ACCGAGACGA CAACATGGGA GAGGAAGTAC
    TGCATCCTGA CTGACAGCCA GCTGGTGCTG CTCAACAGGG AGAAGGAGGT GCCCACCGAG
    GGGTTGCAGG AGCCGCAGGA TGCAACCAAA GGCCGCTGTC TGCGCCGCAC GGTGAGCGTG
    CCCTCCGAGG GCCAGTTCCC CGAGTACCCA CCAGATGGGG CCGCCAAGCT GGAGGTCCCG
    GCAGAGAGAT CTCCTCGCAG ACGGAGCATC TCTGGGACCA GCACATCTGA GAAAACCAAC
    TCCATGGATG CTGCAAATAC CTCTCCTTTC AAAGTATCGG GCTTCTTCAG TAAACGTCTG
    AAAGGGTCCA TCAAGAGGAC AAAGAGTCAG TCAAAGCTGG ACAGGAACAC AAGTTTCCGC
    CTCCCATCCC TCCGCAATGC TGATGACAGG TCACGTGGGC TGCCGAAGCT GAAGGAGTCC
    CGCTCTCATG AGTCCCTGCT GAGCCCCGGC AGCGCAGTGG AAGCCCTGGA CCTTGGGGCA
    GAAGAAAAGG TCTTTGTAAA GCCACTGCAC AGCAGCATCC TCGGACAGGA CTTCTGCTTT
    GAGGTAACCT ACTCCAGCGG CAGTAAATGC TTCAGCTGTT CCTCTGCTGC AGAGAGGGAT
    AAGTGGATGG AAAACCTACG CAGAACAGTG CAGCCGAATA AGGACAACTG CCGTCGAGCT
    GAAAATGTGC TCCGTCTCTG GATCATTGAG GCAAAGGACC TGGCCCCCAA GAAGAAATAC
    TTCTGTGAGC TGTGCCTTGA TGACACTCTC TTTGCCCGCA CCACCAGCAA AACGAAAGCA
    GATAACATTT TCTGGGGGGA GCACTTTGAG TTCTACGGCC TCCCAGCTCT TCACAGCATC
    ACGGTTCACA TCTACAAGGA TGTGGAGAAG AAAAAGAAAA AGGACAAAAA CAATTACGTC
    GGCCTGGTCA ACATTCCCAT GGCCACTGTA ACGGGGCGCC AGTTTGTGGA AAAATGGTAC
    CCAGTCAGCA CACCTACACC CAACAAAGGG AAATCAGGAG GCCCTTCCAT TCGCATCAAG
    TCGCGTTATC AGACCATCAC CATCTTACCC ATGGAGCAAT ACAAGGAGTT TGCAGAGTTT
    GTTACCAGCA ACTACACGAT GCTGTGCTCT GTGCTGGAGC CAGTCATCAG CGTAAGGAAT
    AAGGAAGAGA TGGCTTGTGC AATGGTGCAC ATTCTTCAGA GCACGGGGAG AGCCAAGGAC
    TTCCTAACGG ATCTGGTGAT GTCTGAGGTG GATCGCTGCG GGGAACACGA TGTCTTGATC
    TTCAGGGAGA ACACACTTGC TACCAAAGCT ATTGAGGAAT ACCTGAAGTT GGTAGGGCAG
    AAGTATCTCC ATGATGCGCT AGGGGAGTTT ATCAAGGCTT TGTATGAGTC AGATGAAAAC
    TGTGAAGTGG ACCCCAGCAA ATGTTCATCC AGTGAATTAA CAGACCATCA GAGCAACCTG
    AAAATGTGTT GTGAGCTGGC CTTCTGCAAG ATTATCAATT CCTACTGCGT GTTCCCTCGT
    GAGCTGAAGG AGGTGTTTGC ATCTTGGAAG CAGCAGTGCC TGAGCAGGGG CAAGCAGGAC
    ATCAGCGAGC GGCTGATCAG TGCCTCCCTG TTCCTCCGCT TCCTCTGCCC CGCCATCATG
    TCCCCCAGCC TCTTCAGCCT CATGCAGGAG TATCCTGACG ACCGGACATC GCGCACGCTC
    ACACTCATTG CTAAAGTCAT TCAGAATCTC GCCAATTTTG CTAAGTTTGG TAACAAGGAA
    GAGTACATGG CTTTCATGAA CGACTTTCTG GAACATGAGT GGGGGGGAAT GAAGCGATTT
    CTCCTGGAGA TCTCTAATCC AGATACCATC TCAAACATGC CTGGATTTGA GGGCTATATC
    GACCTGGGCA GAGAGCTCTC AGTTTTGCAT TCCCTCTTAT GGGAGGTTGT GTCCCAACTT
    GATAAGGGTG AAAATTCCTT CCTACAGGCG ACCGTGGCAA AACTGGGACC TCTTCCTCGT
    ATTCTTGCCG ACATCACCAA ATCAATGACC AACCCTACGC CAATACAGCA GCAGCTGAGG
    CGTTTCACTG AGCACAGCTC TAGTCCAAAC GTCAGTGGCA GTCTCTCCTC AGGGCTTCAG
    AAGATATTCG AGGACCCCAC TGATGGTGAC TTGCATAAAC TGAAGTCTCC AACCCAGGAA
    AACATAGATG GCTATTTCAG GGGCAAGACC TTGCTGTTGG TTCAGCAGGC GTCCACCCAG
    AGCATGACTT ACTCAGATAA GGACGAAAGA GAGAGCATCT TGCCCAACGG ACGTAGCATC
    TCCCTCATGG ACCTGCAGGA TCCCCATACC GCTCACAGCG ACCACGCTTC CATGATGCAC
    GACGTGCCTT TGCGCTTGGC CGGCAGCCAG CTCTCCATCA CCCAGGTGGC CAACATCAAG
    CAGCTGTGGG AGACCCAGAG CACGCCCCAA AGCGCTCCCC AGGTGAGGAG GCCGCTGCAC
    CCGGCTTTGA ACCAGCAGGG CAGCCTCCAG CCTCTGTCAT TCCAGAACCC GGTGTACCAC
    CTCAACAACC CACCTGCGCC GTTGCCCAAG GCCTCCGTGG ACTCCAGCCT GGAGAACCTG
    AGCACCGCCA GCTCCCAGAG CCGAAGCAAC AGCGAAGACT TCAAGCTCAG CGGGCCCAGC
    AACAGCAGCA TGGAGGACTT CACCAAGCGC AGCACGCAGA GCGAGGACTT CTCTAGGCGG
    CACACGGTCC CGGACAAGCA CGTCCCCATC GCGTTGCCCC GCCAGAACAG CAGCAGCCAG
    GCACAGATCC GCAAAATGGA CCAGGCCGGG TTAGGGGCCA GGGCCAAAGC GCCGCAGTCG
    CTGCCGCACA GCGCGTCCCT GCGCAGCACG GGCAGCATGT CGGCCGTGTC GGGGGCCGTG
    GTGACTGAGC CCGTCCAGAA CGGCAGCCGC TCCCGGCAGC AGTCGTCCTC CTCCAGGGAG
    AGCCCTGTGC CGAAGGTGAG GGCCATTCAG AGGCAGCAAA CGCAGCAGGT CCAGTCTCCT
    GTGGACTCAG CCACAATGTC ACCAGTGGAA AGGACAGCAG CGTGGGTTCT CAATAACGGG
    CAGTACGAGG AGGTCGAGGA GGACACAGAG CAGAACCCAG ATGAAGCCAA GCATGCTGAG
    AAGTACGAAC AAGAGATAGC AAAGCTGAAG GAACGCTTGA AAGTGTCGAG CCGCCGGCTG
    GAGGAGTACG AGCGGCGGCT CCTGGTGCAG GAGCAGCAAA TGCAGAAGCT GCTGATGGAG
    TACAAGTCCA GGCTGGAGGA CAGCGAGGAG AGACTGCGCA GGCAACAGGA GGAGAAGGAC
    AGCCAGATGA AAAGCATTAT CAGCAGGCTC ATGGCAGTTG AAGAGGAACT GAAGAAAGAT
    CATGCAGAGA TGCAAGCTGT CATCGATGCA AAGCAGAAAA TTATTGATGC ACAGGTCATT
    AATGGGAATG AGATAATTCA AACAGGAGAA GCGGATCGTG TCCCTGGACT CTGCCAACAC
    GCGGCTGATG AGTGCCCTGA CGCAGGTGAA GGAGCGCTAC AGCATGCAAG TCCGCAATGG
    CATCTCCCCC ACCAACCCCA CCAAGCTTTC CATCACGGAG AATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39045
    Clone ID Related Accession (Same CDS sequence) XM_004943250.2
    Accession Version XM_004943250.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3819bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product ras GTPase-activating protein nGAP isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004943250.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGGCGG CCCCCGCGGC GAGCGAGCCG GCGGCCTGGG CGGCCGAGGC GCTGGCGGAG 
    CCGGAGCTGG GCTCGGACGA GCTGGAGGCG GCGGGCGGAG CGCTGGACCG CGTCCTGCTG
    GACTCGGTGT GCCAGCAGCA GGGCTGGGTC CGCGTCTACG ATGTAAAAGG ACCACCTACG
    CACAGGCTGT CCTGCGGCCA GTCCCCCTAC ACCGAGACGA CAACATGGGA GAGGAAGTAC
    TGCATCCTGA CTGACAGCCA GCTGGTGCTG CTCAACAGGG AGAAGGAGGT GCCCACCGAG
    GGGTTGCAGG AGCCGCAGGA TGCAACCAAA GGCCGCTGTC TGCGCCGCAC GGTGAGCGTG
    CCCTCCGAGG GCCAGTTCCC CGAGTACCCA CCAGATGGGG CCGCCAAGCT GGAGGTCCCG
    GCAGAGAGAT CTCCTCGCAG ACGGAGCATC TCTGGGACCA GCACATCTGA GAAAACCAAC
    TCCATGGATG CTGCAAATAC CTCTCCTTTC AAAGTATCGG GCTTCTTCAG TAAACGTCTG
    AAAGGGTCCA TCAAGAGGAC AAAGAGTCAG TCAAAGCTGG ACAGGAACAC AAGTTTCCGC
    CTCCCATCCC TCCGCAATGC TGATGACAGG TCACGTGGGC TGCCGAAGCT GAAGGAGTCC
    CGCTCTCATG AGTCCCTGCT GAGCCCCGGC AGCGCAGTGG AAGCCCTGGA CCTTGGGGCA
    GAAGAAAAGG TCTTTGTAAA GCCACTGCAC AGCAGCATCC TCGGACAGGA CTTCTGCTTT
    GAGGTAACCT ACTCCAGCGG CAGTAAATGC TTCAGCTGTT CCTCTGCTGC AGAGAGGGAT
    AAGTGGATGG AAAACCTACG CAGAACAGTG CAGCCGAATA AGGACAACTG CCGTCGAGCT
    GAAAATGTGC TCCGTCTCTG GATCATTGAG GCAAAGGACC TGGCCCCCAA GAAGAAATAC
    TTCTGTGAGC TGTGCCTTGA TGACACTCTC TTTGCCCGCA CCACCAGCAA AACGAAAGCA
    GATAACATTT TCTGGGGGGA GCACTTTGAG TTCTACGGCC TCCCAGCTCT TCACAGCATC
    ACGGTTCACA TCTACAAGGA TGTGGAGAAG AAAAAGAAAA AGGACAAAAA CAATTACGTC
    GGCCTGGTCA ACATTCCCAT GGCCACTGTA ACGGGGCGCC AGTTTGTGGA AAAATGGTAC
    CCAGTCAGCA CACCTACACC CAACAAAGGG AAATCAGGAG GCCCTTCCAT TCGCATCAAG
    TCGCGTTATC AGACCATCAC CATCTTACCC ATGGAGCAAT ACAAGGAGTT TGCAGAGTTT
    GTTACCAGCA ACTACACGAT GCTGTGCTCT GTGCTGGAGC CAGTCATCAG CGTAAGGAAT
    AAGGAAGAGA TGGCTTGTGC AATGGTGCAC ATTCTTCAGA GCACGGGGAG AGCCAAGGAC
    TTCCTAACGG ATCTGGTGAT GTCTGAGGTG GATCGCTGCG GGGAACACGA TGTCTTGATC
    TTCAGGGAGA ACACACTTGC TACCAAAGCT ATTGAGGAAT ACCTGAAGTT GGTAGGGCAG
    AAGTATCTCC ATGATGCGCT AGGGGAGTTT ATCAAGGCTT TGTATGAGTC AGATGAAAAC
    TGTGAAGTGG ACCCCAGCAA ATGTTCATCC AGTGAATTAA CAGACCATCA GAGCAACCTG
    AAAATGTGTT GTGAGCTGGC CTTCTGCAAG ATTATCAATT CCTACTGCGT GTTCCCTCGT
    GAGCTGAAGG AGGTGTTTGC ATCTTGGAAG CAGCAGTGCC TGAGCAGGGG CAAGCAGGAC
    ATCAGCGAGC GGCTGATCAG TGCCTCCCTG TTCCTCCGCT TCCTCTGCCC CGCCATCATG
    TCCCCCAGCC TCTTCAGCCT CATGCAGGAG TATCCTGACG ACCGGACATC GCGCACGCTC
    ACACTCATTG CTAAAGTCAT TCAGAATCTC GCCAATTTTG CTAAGTTTGG TAACAAGGAA
    GAGTACATGG CTTTCATGAA CGACTTTCTG GAACATGAGT GGGGGGGAAT GAAGCGATTT
    CTCCTGGAGA TCTCTAATCC AGATACCATC TCAAACATGC CTGGATTTGA GGGCTATATC
    GACCTGGGCA GAGAGCTCTC AGTTTTGCAT TCCCTCTTAT GGGAGGTTGT GTCCCAACTT
    GATAAGGGTG AAAATTCCTT CCTACAGGCG ACCGTGGCAA AACTGGGACC TCTTCCTCGT
    ATTCTTGCCG ACATCACCAA ATCAATGACC AACCCTACGC CAATACAGCA GCAGCTGAGG
    CGTTTCACTG AGCACAGCTC TAGTCCAAAC GTCAGTGGCA GTCTCTCCTC AGGGCTTCAG
    AAGATATTCG AGGACCCCAC TGATGGTGAC TTGCATAAAC TGAAGTCTCC AACCCAGGAA
    AACATAGATG GCTATTTCAG GGGCAAGACC TTGCTGTTGG TTCAGCAGGC GTCCACCCAG
    AGCATGACTT ACTCAGATAA GGACGAAAGA GAGAGCATCT TGCCCAACGG ACGTAGCATC
    TCCCTCATGG ACCTGCAGGA TCCCCATACC GCTCACAGCG ACCACGCTTC CATGATGCAC
    GACGTGCCTT TGCGCTTGGC CGGCAGCCAG CTCTCCATCA CCCAGGTGGC CAACATCAAG
    CAGCTGTGGG AGACCCAGAG CACGCCCCAA AGCGCTCCCC AGGTGAGGAG GCCGCTGCAC
    CCGGCTTTGA ACCAGCAGGG CAGCCTCCAG CCTCTGTCAT TCCAGAACCC GGTGTACCAC
    CTCAACAACC CACCTGCGCC GTTGCCCAAG GCCTCCGTGG ACTCCAGCCT GGAGAACCTG
    AGCACCGCCA GCTCCCAGAG CCGAAGCAAC AGCGAAGACT TCAAGCTCAG CGGGCCCAGC
    AACAGCAGCA TGGAGGACTT CACCAAGCGC AGCACGCAGA GCGAGGACTT CTCTAGGCGG
    CACACGGTCC CGGACAAGCA CGTCCCCATC GCGTTGCCCC GCCAGAACAG CAGCAGCCAG
    GCACAGATCC GCAAAATGGA CCAGGCCGGG TTAGGGGCCA GGGCCAAAGC GCCGCAGTCG
    CTGCCGCACA GCGCGTCCCT GCGCAGCACG GGCAGCATGT CGGCCGTGTC GGGGGCCGTG
    GTGACTGAGC CCGTCCAGAA CGGCAGCCGC TCCCGGCAGC AGTCGTCCTC CTCCAGGGAG
    AGCCCTGTGC CGAAGGTGAG GGCCATTCAG AGGCAGCAAA CGCAGCAGGT CCAGTCTCCT
    GTGGACTCAG CCACAATGTC ACCAGTGGAA AGGACAGCAG CGTGGGTTCT CAATAACGGG
    CAGTACGAGG AGGTCGAGGA GGACACAGAG CAGAACCCAG ATGAAGCCAA GCATGCTGAG
    AAGTACGAAC AAGAGATAGC AAAGCTGAAG GAACGCTTGA AAGTGTCGAG CCGCCGGCTG
    GAGGAGTACG AGCGGCGGCT CCTGGTGCAG GAGCAGCAAA TGCAGAAGCT GCTGATGGAG
    TACAAGTCCA GGCTGGAGGA CAGCGAGGAG AGACTGCGCA GGCAACAGGA GGAGAAGGAC
    AGCCAGATGA AAAGCATTAT CAGCAGGCTC ATGGCAGTTG AAGAGGAACT GAAGAAAGAT
    CATGCAGAGA TGCAAGCTGT CATCGATGCA AAGCAGAAAA TTATTGATGC ACAGGAGAAG
    CGGATCGTGT CCCTGGACTC TGCCAACACG CGGCTGATGA GTGCCCTGAC GCAGGTGAAG
    GAGCGCTACA GCATGCAAGT CCGCAATGGC ATCTCCCCCA CCAACCCCAC CAAGCTTTCC
    ATCACGGAGA ATGGTGAATT CAAAAACAGC AGCTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004943307.1
    CDS77..3895
    Translation

    Target ORF information:

    RefSeq Version XM_004943250.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004943250.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGGCGG CCCCCGCGGC GAGCGAGCCG GCGGCCTGGG CGGCCGAGGC GCTGGCGGAG 
    CCGGAGCTGG GCTCGGACGA GCTGGAGGCG GCGGGCGGAG CGCTGGACCG CGTCCTGCTG
    GACTCGGTGT GCCAGCAGCA GGGCTGGGTC CGCGTCTACG ATGTAAAAGG ACCACCTACG
    CACAGGCTGT CCTGCGGCCA GTCCCCCTAC ACCGAGACGA CAACATGGGA GAGGAAGTAC
    TGCATCCTGA CTGACAGCCA GCTGGTGCTG CTCAACAGGG AGAAGGAGGT GCCCACCGAG
    GGGTTGCAGG AGCCGCAGGA TGCAACCAAA GGCCGCTGTC TGCGCCGCAC GGTGAGCGTG
    CCCTCCGAGG GCCAGTTCCC CGAGTACCCA CCAGATGGGG CCGCCAAGCT GGAGGTCCCG
    GCAGAGAGAT CTCCTCGCAG ACGGAGCATC TCTGGGACCA GCACATCTGA GAAAACCAAC
    TCCATGGATG CTGCAAATAC CTCTCCTTTC AAAGTATCGG GCTTCTTCAG TAAACGTCTG
    AAAGGGTCCA TCAAGAGGAC AAAGAGTCAG TCAAAGCTGG ACAGGAACAC AAGTTTCCGC
    CTCCCATCCC TCCGCAATGC TGATGACAGG TCACGTGGGC TGCCGAAGCT GAAGGAGTCC
    CGCTCTCATG AGTCCCTGCT GAGCCCCGGC AGCGCAGTGG AAGCCCTGGA CCTTGGGGCA
    GAAGAAAAGG TCTTTGTAAA GCCACTGCAC AGCAGCATCC TCGGACAGGA CTTCTGCTTT
    GAGGTAACCT ACTCCAGCGG CAGTAAATGC TTCAGCTGTT CCTCTGCTGC AGAGAGGGAT
    AAGTGGATGG AAAACCTACG CAGAACAGTG CAGCCGAATA AGGACAACTG CCGTCGAGCT
    GAAAATGTGC TCCGTCTCTG GATCATTGAG GCAAAGGACC TGGCCCCCAA GAAGAAATAC
    TTCTGTGAGC TGTGCCTTGA TGACACTCTC TTTGCCCGCA CCACCAGCAA AACGAAAGCA
    GATAACATTT TCTGGGGGGA GCACTTTGAG TTCTACGGCC TCCCAGCTCT TCACAGCATC
    ACGGTTCACA TCTACAAGGA TGTGGAGAAG AAAAAGAAAA AGGACAAAAA CAATTACGTC
    GGCCTGGTCA ACATTCCCAT GGCCACTGTA ACGGGGCGCC AGTTTGTGGA AAAATGGTAC
    CCAGTCAGCA CACCTACACC CAACAAAGGG AAATCAGGAG GCCCTTCCAT TCGCATCAAG
    TCGCGTTATC AGACCATCAC CATCTTACCC ATGGAGCAAT ACAAGGAGTT TGCAGAGTTT
    GTTACCAGCA ACTACACGAT GCTGTGCTCT GTGCTGGAGC CAGTCATCAG CGTAAGGAAT
    AAGGAAGAGA TGGCTTGTGC AATGGTGCAC ATTCTTCAGA GCACGGGGAG AGCCAAGGAC
    TTCCTAACGG ATCTGGTGAT GTCTGAGGTG GATCGCTGCG GGGAACACGA TGTCTTGATC
    TTCAGGGAGA ACACACTTGC TACCAAAGCT ATTGAGGAAT ACCTGAAGTT GGTAGGGCAG
    AAGTATCTCC ATGATGCGCT AGGGGAGTTT ATCAAGGCTT TGTATGAGTC AGATGAAAAC
    TGTGAAGTGG ACCCCAGCAA ATGTTCATCC AGTGAATTAA CAGACCATCA GAGCAACCTG
    AAAATGTGTT GTGAGCTGGC CTTCTGCAAG ATTATCAATT CCTACTGCGT GTTCCCTCGT
    GAGCTGAAGG AGGTGTTTGC ATCTTGGAAG CAGCAGTGCC TGAGCAGGGG CAAGCAGGAC
    ATCAGCGAGC GGCTGATCAG TGCCTCCCTG TTCCTCCGCT TCCTCTGCCC CGCCATCATG
    TCCCCCAGCC TCTTCAGCCT CATGCAGGAG TATCCTGACG ACCGGACATC GCGCACGCTC
    ACACTCATTG CTAAAGTCAT TCAGAATCTC GCCAATTTTG CTAAGTTTGG TAACAAGGAA
    GAGTACATGG CTTTCATGAA CGACTTTCTG GAACATGAGT GGGGGGGAAT GAAGCGATTT
    CTCCTGGAGA TCTCTAATCC AGATACCATC TCAAACATGC CTGGATTTGA GGGCTATATC
    GACCTGGGCA GAGAGCTCTC AGTTTTGCAT TCCCTCTTAT GGGAGGTTGT GTCCCAACTT
    GATAAGGGTG AAAATTCCTT CCTACAGGCG ACCGTGGCAA AACTGGGACC TCTTCCTCGT
    ATTCTTGCCG ACATCACCAA ATCAATGACC AACCCTACGC CAATACAGCA GCAGCTGAGG
    CGTTTCACTG AGCACAGCTC TAGTCCAAAC GTCAGTGGCA GTCTCTCCTC AGGGCTTCAG
    AAGATATTCG AGGACCCCAC TGATGGTGAC TTGCATAAAC TGAAGTCTCC AACCCAGGAA
    AACATAGATG GCTATTTCAG GGGCAAGACC TTGCTGTTGG TTCAGCAGGC GTCCACCCAG
    AGCATGACTT ACTCAGATAA GGACGAAAGA GAGAGCATCT TGCCCAACGG ACGTAGCATC
    TCCCTCATGG ACCTGCAGGA TCCCCATACC GCTCACAGCG ACCACGCTTC CATGATGCAC
    GACGTGCCTT TGCGCTTGGC CGGCAGCCAG CTCTCCATCA CCCAGGTGGC CAACATCAAG
    CAGCTGTGGG AGACCCAGAG CACGCCCCAA AGCGCTCCCC AGGTGAGGAG GCCGCTGCAC
    CCGGCTTTGA ACCAGCAGGG CAGCCTCCAG CCTCTGTCAT TCCAGAACCC GGTGTACCAC
    CTCAACAACC CACCTGCGCC GTTGCCCAAG GCCTCCGTGG ACTCCAGCCT GGAGAACCTG
    AGCACCGCCA GCTCCCAGAG CCGAAGCAAC AGCGAAGACT TCAAGCTCAG CGGGCCCAGC
    AACAGCAGCA TGGAGGACTT CACCAAGCGC AGCACGCAGA GCGAGGACTT CTCTAGGCGG
    CACACGGTCC CGGACAAGCA CGTCCCCATC GCGTTGCCCC GCCAGAACAG CAGCAGCCAG
    GCACAGATCC GCAAAATGGA CCAGGCCGGG TTAGGGGCCA GGGCCAAAGC GCCGCAGTCG
    CTGCCGCACA GCGCGTCCCT GCGCAGCACG GGCAGCATGT CGGCCGTGTC GGGGGCCGTG
    GTGACTGAGC CCGTCCAGAA CGGCAGCCGC TCCCGGCAGC AGTCGTCCTC CTCCAGGGAG
    AGCCCTGTGC CGAAGGTGAG GGCCATTCAG AGGCAGCAAA CGCAGCAGGT CCAGTCTCCT
    GTGGACTCAG CCACAATGTC ACCAGTGGAA AGGACAGCAG CGTGGGTTCT CAATAACGGG
    CAGTACGAGG AGGTCGAGGA GGACACAGAG CAGAACCCAG ATGAAGCCAA GCATGCTGAG
    AAGTACGAAC AAGAGATAGC AAAGCTGAAG GAACGCTTGA AAGTGTCGAG CCGCCGGCTG
    GAGGAGTACG AGCGGCGGCT CCTGGTGCAG GAGCAGCAAA TGCAGAAGCT GCTGATGGAG
    TACAAGTCCA GGCTGGAGGA CAGCGAGGAG AGACTGCGCA GGCAACAGGA GGAGAAGGAC
    AGCCAGATGA AAAGCATTAT CAGCAGGCTC ATGGCAGTTG AAGAGGAACT GAAGAAAGAT
    CATGCAGAGA TGCAAGCTGT CATCGATGCA AAGCAGAAAA TTATTGATGC ACAGGAGAAG
    CGGATCGTGT CCCTGGACTC TGCCAACACG CGGCTGATGA GTGCCCTGAC GCAGGTGAAG
    GAGCGCTACA GCATGCAAGT CCGCAATGGC ATCTCCCCCA CCAACCCCAC CAAGCTTTCC
    ATCACGGAGA ATGGTGAATT CAAAAACAGC AGCTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39046
    Clone ID Related Accession (Same CDS sequence) XM_015290514.1
    Accession Version XM_015290514.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3798bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product ras GTPase-activating protein nGAP isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGGCGG CCCCCGCGGC GAGCGAGCCG GCGGCCTGGG CGGCCGAGGC GCTGGCGGAG 
    CCGGAGCTGG GCTCGGACGA GCTGGAGGCG GCGGGCGGAG CGCTGGACCG CGTCCTGCTG
    GACTCGGTGT GCCAGCAGCA GGGCTGGGTC CGCGTCTACG ATGTAAAAGG ACCACCTACG
    CACAGGCTGT CCTGCGGCCA GTCCCCCTAC ACCGAGACGA CAACATGGGA GAGGAAGTAC
    TGCATCCTGA CTGACAGCCA GCTGGTGCTG CTCAACAGGG AGAAGGAGGT GCCCACCGAG
    GGGTTGCAGG AGCCGCAGGA TGCAACCAAA GGCCGCTGTC TGCGCCGCAC GGTGAGCGTG
    CCCTCCGAGG GCCAGTTCCC CGAGTACCCA CCAGATGGGG CCGCCAAGCT GGAGGTCCCG
    GCAGAGAGAT CTCCTCGCAG ACGGAGCATC TCTGGGACCA GCACATCTGA GAAAACCAAC
    TCCATGGATG CTGCAAATAC CTCTCCTTTC AAAGTATCGG GCTTCTTCAG TAAACGTCTG
    AAAGGGTCCA TCAAGAGGAC AAAGAGTCAG TCAAAGCTGG ACAGGAACAC AAGTTTCCGC
    CTCCCATCCC TCCGCAATGC TGATGACAGG TCACGTGGGC TGCCGAAGCT GAAGGAGTCC
    CGCTCTCATG AGTCCCTGCT GAGCCCCGGC AGCGCAGTGG AAGCCCTGGA CCTTGGGGCA
    GAAGAAAAGG TCTTTGTAAA GCCACTGCAC AGCAGCATCC TCGGACAGGA CTTCTGCTTT
    GAGGTAACCT ACTCCAGCGG CAGTAAATGC TTCAGCTGTT CCTCTGCTGC AGAGAGGGAT
    AAGTGGATGG AAAACCTACG CAGAACAGTG CAGCCGAATA AGGACAACTG CCGTCGAGCT
    GAAAATGTGC TCCGTCTCTG GATCATTGAG GCAAAGGACC TGGCCCCCAA GAAGAAATAC
    TTCTGTGAGC TGTGCCTTGA TGACACTCTC TTTGCCCGCA CCACCAGCAA AACGAAAGCA
    GATAACATTT TCTGGGGGGA GCACTTTGAG TTCTACGGCC TCCCAGCTCT TCACAGCATC
    ACGGTTCACA TCTACAAGGA TGTGGAGAAG AAAAAGAAAA AGGACAAAAA CAATTACGTC
    GGCCTGGTCA ACATTCCCAT GGCCACTGTA ACGGGGCGCC AGTTTGTGGA AAAATGGTAC
    CCAGTCAGCA CACCTACACC CAACAAAGGG AAATCAGGAG GCCCTTCCAT TCGCATCAAG
    TCGCGTTATC AGACCATCAC CATCTTACCC ATGGAGCAAT ACAAGGAGTT TGCAGAGTTT
    GTTACCAGCA ACTACACGAT GCTGTGCTCT GTGCTGGAGC CAGTCATCAG CGTAAGGAAT
    AAGGAAGAGA TGGCTTGTGC AATGGTGCAC ATTCTTCAGA GCACGGGGAG AGCCAAGGAC
    TTCCTAACGG ATCTGGTGAT GTCTGAGGTG GATCGCTGCG GGGAACACGA TGTCTTGATC
    TTCAGGGAGA ACACACTTGC TACCAAAGCT ATTGAGGAAT ACCTGAAGTT GGTAGGGCAG
    AAGTATCTCC ATGATGCGCT AGGGGAGTTT ATCAAGGCTT TGTATGAGTC AGATGAAAAC
    TGTGAAGTGG ACCCCAGCAA ATGTTCATCC AGTGAATTAA CAGACCATCA GAGCAACCTG
    AAAATGTGTT GTGAGCTGGC CTTCTGCAAG ATTATCAATT CCTACTGCGT GTTCCCTCGT
    GAGCTGAAGG AGGTGTTTGC ATCTTGGAAG CAGCAGTGCC TGAGCAGGGG CAAGCAGGAC
    ATCAGCGAGC GGCTGATCAG TGCCTCCCTG TTCCTCCGCT TCCTCTGCCC CGCCATCATG
    TCCCCCAGCC TCTTCAGCCT CATGCAGGAG TATCCTGACG ACCGGACATC GCGCACGCTC
    ACACTCATTG CTAAAGTCAT TCAGAATCTC GCCAATTTTG CTAAGTTTGG TAACAAGGAA
    GAGTACATGG CTTTCATGAA CGACTTTCTG GAACATGAGT GGGGGGGAAT GAAGCGATTT
    CTCCTGGAGA TCTCTAATCC AGATACCATC TCAAACATGC CTGGATTTGA GGGCTATATC
    GACCTGGGCA GAGAGCTCTC AGTTTTGCAT TCCCTCTTAT GGGAGGTTGT GTCCCAACTT
    GATAAGGCGA CCGTGGCAAA ACTGGGACCT CTTCCTCGTA TTCTTGCCGA CATCACCAAA
    TCAATGACCA ACCCTACGCC AATACAGCAG CAGCTGAGGC GTTTCACTGA GCACAGCTCT
    AGTCCAAACG TCAGTGGCAG TCTCTCCTCA GGGCTTCAGA AGATATTCGA GGACCCCACT
    GATGGTGACT TGCATAAACT GAAGTCTCCA ACCCAGGAAA ACATAGATGG CTATTTCAGG
    GGCAAGACCT TGCTGTTGGT TCAGCAGGCG TCCACCCAGA GCATGACTTA CTCAGATAAG
    GACGAAAGAG AGAGCATCTT GCCCAACGGA CGTAGCATCT CCCTCATGGA CCTGCAGGAT
    CCCCATACCG CTCACAGCGA CCACGCTTCC ATGATGCACG ACGTGCCTTT GCGCTTGGCC
    GGCAGCCAGC TCTCCATCAC CCAGGTGGCC AACATCAAGC AGCTGTGGGA GACCCAGAGC
    ACGCCCCAAA GCGCTCCCCA GGTGAGGAGG CCGCTGCACC CGGCTTTGAA CCAGCAGGGC
    AGCCTCCAGC CTCTGTCATT CCAGAACCCG GTGTACCACC TCAACAACCC ACCTGCGCCG
    TTGCCCAAGG CCTCCGTGGA CTCCAGCCTG GAGAACCTGA GCACCGCCAG CTCCCAGAGC
    CGAAGCAACA GCGAAGACTT CAAGCTCAGC GGGCCCAGCA ACAGCAGCAT GGAGGACTTC
    ACCAAGCGCA GCACGCAGAG CGAGGACTTC TCTAGGCGGC ACACGGTCCC GGACAAGCAC
    GTCCCCATCG CGTTGCCCCG CCAGAACAGC AGCAGCCAGG CACAGATCCG CAAAATGGAC
    CAGGCCGGGT TAGGGGCCAG GGCCAAAGCG CCGCAGTCGC TGCCGCACAG CGCGTCCCTG
    CGCAGCACGG GCAGCATGTC GGCCGTGTCG GGGGCCGTGG TGACTGAGCC CGTCCAGAAC
    GGCAGCCGCT CCCGGCAGCA GTCGTCCTCC TCCAGGGAGA GCCCTGTGCC GAAGGTGAGG
    GCCATTCAGA GGCAGCAAAC GCAGCAGGTC CAGTCTCCTG TGGACTCAGC CACAATGTCA
    CCAGTGGAAA GGACAGCAGC GTGGGTTCTC AATAACGGGC AGTACGAGGA GGTCGAGGAG
    GACACAGAGC AGAACCCAGA TGAAGCCAAG CATGCTGAGA AGTACGAACA AGAGATAGCA
    AAGCTGAAGG AACGCTTGAA AGTGTCGAGC CGCCGGCTGG AGGAGTACGA GCGGCGGCTC
    CTGGTGCAGG AGCAGCAAAT GCAGAAGCTG CTGATGGAGT ACAAGTCCAG GCTGGAGGAC
    AGCGAGGAGA GACTGCGCAG GCAACAGGAG GAGAAGGACA GCCAGATGAA AAGCATTATC
    AGCAGGCTCA TGGCAGTTGA AGAGGAACTG AAGAAAGATC ATGCAGAGAT GCAAGCTGTC
    ATCGATGCAA AGCAGAAAAT TATTGATGCA CAGGAGAAGC GGATCGTGTC CCTGGACTCT
    GCCAACACGC GGCTGATGAG TGCCCTGACG CAGGTGAAGG AGCGCTACAG CATGCAAGTC
    CGCAATGGCA TCTCCCCCAC CAACCCCACC AAGCTTTCCA TCACGGAGAA TGGTGAATTC
    AAAAACAGCA GCTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015146000.1
    CDS77..3874
    Translation

    Target ORF information:

    RefSeq Version XM_015290514.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus RAS protein activator like 2 (RASAL2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015290514.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGGCGG CCCCCGCGGC GAGCGAGCCG GCGGCCTGGG CGGCCGAGGC GCTGGCGGAG 
    CCGGAGCTGG GCTCGGACGA GCTGGAGGCG GCGGGCGGAG CGCTGGACCG CGTCCTGCTG
    GACTCGGTGT GCCAGCAGCA GGGCTGGGTC CGCGTCTACG ATGTAAAAGG ACCACCTACG
    CACAGGCTGT CCTGCGGCCA GTCCCCCTAC ACCGAGACGA CAACATGGGA GAGGAAGTAC
    TGCATCCTGA CTGACAGCCA GCTGGTGCTG CTCAACAGGG AGAAGGAGGT GCCCACCGAG
    GGGTTGCAGG AGCCGCAGGA TGCAACCAAA GGCCGCTGTC TGCGCCGCAC GGTGAGCGTG
    CCCTCCGAGG GCCAGTTCCC CGAGTACCCA CCAGATGGGG CCGCCAAGCT GGAGGTCCCG
    GCAGAGAGAT CTCCTCGCAG ACGGAGCATC TCTGGGACCA GCACATCTGA GAAAACCAAC
    TCCATGGATG CTGCAAATAC CTCTCCTTTC AAAGTATCGG GCTTCTTCAG TAAACGTCTG
    AAAGGGTCCA TCAAGAGGAC AAAGAGTCAG TCAAAGCTGG ACAGGAACAC AAGTTTCCGC
    CTCCCATCCC TCCGCAATGC TGATGACAGG TCACGTGGGC TGCCGAAGCT GAAGGAGTCC
    CGCTCTCATG AGTCCCTGCT GAGCCCCGGC AGCGCAGTGG AAGCCCTGGA CCTTGGGGCA
    GAAGAAAAGG TCTTTGTAAA GCCACTGCAC AGCAGCATCC TCGGACAGGA CTTCTGCTTT
    GAGGTAACCT ACTCCAGCGG CAGTAAATGC TTCAGCTGTT CCTCTGCTGC AGAGAGGGAT
    AAGTGGATGG AAAACCTACG CAGAACAGTG CAGCCGAATA AGGACAACTG CCGTCGAGCT
    GAAAATGTGC TCCGTCTCTG GATCATTGAG GCAAAGGACC TGGCCCCCAA GAAGAAATAC
    TTCTGTGAGC TGTGCCTTGA TGACACTCTC TTTGCCCGCA CCACCAGCAA AACGAAAGCA
    GATAACATTT TCTGGGGGGA GCACTTTGAG TTCTACGGCC TCCCAGCTCT TCACAGCATC
    ACGGTTCACA TCTACAAGGA TGTGGAGAAG AAAAAGAAAA AGGACAAAAA CAATTACGTC
    GGCCTGGTCA ACATTCCCAT GGCCACTGTA ACGGGGCGCC AGTTTGTGGA AAAATGGTAC
    CCAGTCAGCA CACCTACACC CAACAAAGGG AAATCAGGAG GCCCTTCCAT TCGCATCAAG
    TCGCGTTATC AGACCATCAC CATCTTACCC ATGGAGCAAT ACAAGGAGTT TGCAGAGTTT
    GTTACCAGCA ACTACACGAT GCTGTGCTCT GTGCTGGAGC CAGTCATCAG CGTAAGGAAT
    AAGGAAGAGA TGGCTTGTGC AATGGTGCAC ATTCTTCAGA GCACGGGGAG AGCCAAGGAC
    TTCCTAACGG ATCTGGTGAT GTCTGAGGTG GATCGCTGCG GGGAACACGA TGTCTTGATC
    TTCAGGGAGA ACACACTTGC TACCAAAGCT ATTGAGGAAT ACCTGAAGTT GGTAGGGCAG
    AAGTATCTCC ATGATGCGCT AGGGGAGTTT ATCAAGGCTT TGTATGAGTC AGATGAAAAC
    TGTGAAGTGG ACCCCAGCAA ATGTTCATCC AGTGAATTAA CAGACCATCA GAGCAACCTG
    AAAATGTGTT GTGAGCTGGC CTTCTGCAAG ATTATCAATT CCTACTGCGT GTTCCCTCGT
    GAGCTGAAGG AGGTGTTTGC ATCTTGGAAG CAGCAGTGCC TGAGCAGGGG CAAGCAGGAC
    ATCAGCGAGC GGCTGATCAG TGCCTCCCTG TTCCTCCGCT TCCTCTGCCC CGCCATCATG
    TCCCCCAGCC TCTTCAGCCT CATGCAGGAG TATCCTGACG ACCGGACATC GCGCACGCTC
    ACACTCATTG CTAAAGTCAT TCAGAATCTC GCCAATTTTG CTAAGTTTGG TAACAAGGAA
    GAGTACATGG CTTTCATGAA CGACTTTCTG GAACATGAGT GGGGGGGAAT GAAGCGATTT
    CTCCTGGAGA TCTCTAATCC AGATACCATC TCAAACATGC CTGGATTTGA GGGCTATATC
    GACCTGGGCA GAGAGCTCTC AGTTTTGCAT TCCCTCTTAT GGGAGGTTGT GTCCCAACTT
    GATAAGGCGA CCGTGGCAAA ACTGGGACCT CTTCCTCGTA TTCTTGCCGA CATCACCAAA
    TCAATGACCA ACCCTACGCC AATACAGCAG CAGCTGAGGC GTTTCACTGA GCACAGCTCT
    AGTCCAAACG TCAGTGGCAG TCTCTCCTCA GGGCTTCAGA AGATATTCGA GGACCCCACT
    GATGGTGACT TGCATAAACT GAAGTCTCCA ACCCAGGAAA ACATAGATGG CTATTTCAGG
    GGCAAGACCT TGCTGTTGGT TCAGCAGGCG TCCACCCAGA GCATGACTTA CTCAGATAAG
    GACGAAAGAG AGAGCATCTT GCCCAACGGA CGTAGCATCT CCCTCATGGA CCTGCAGGAT
    CCCCATACCG CTCACAGCGA CCACGCTTCC ATGATGCACG ACGTGCCTTT GCGCTTGGCC
    GGCAGCCAGC TCTCCATCAC CCAGGTGGCC AACATCAAGC AGCTGTGGGA GACCCAGAGC
    ACGCCCCAAA GCGCTCCCCA GGTGAGGAGG CCGCTGCACC CGGCTTTGAA CCAGCAGGGC
    AGCCTCCAGC CTCTGTCATT CCAGAACCCG GTGTACCACC TCAACAACCC ACCTGCGCCG
    TTGCCCAAGG CCTCCGTGGA CTCCAGCCTG GAGAACCTGA GCACCGCCAG CTCCCAGAGC
    CGAAGCAACA GCGAAGACTT CAAGCTCAGC GGGCCCAGCA ACAGCAGCAT GGAGGACTTC
    ACCAAGCGCA GCACGCAGAG CGAGGACTTC TCTAGGCGGC ACACGGTCCC GGACAAGCAC
    GTCCCCATCG CGTTGCCCCG CCAGAACAGC AGCAGCCAGG CACAGATCCG CAAAATGGAC
    CAGGCCGGGT TAGGGGCCAG GGCCAAAGCG CCGCAGTCGC TGCCGCACAG CGCGTCCCTG
    CGCAGCACGG GCAGCATGTC GGCCGTGTCG GGGGCCGTGG TGACTGAGCC CGTCCAGAAC
    GGCAGCCGCT CCCGGCAGCA GTCGTCCTCC TCCAGGGAGA GCCCTGTGCC GAAGGTGAGG
    GCCATTCAGA GGCAGCAAAC GCAGCAGGTC CAGTCTCCTG TGGACTCAGC CACAATGTCA
    CCAGTGGAAA GGACAGCAGC GTGGGTTCTC AATAACGGGC AGTACGAGGA GGTCGAGGAG
    GACACAGAGC AGAACCCAGA TGAAGCCAAG CATGCTGAGA AGTACGAACA AGAGATAGCA
    AAGCTGAAGG AACGCTTGAA AGTGTCGAGC CGCCGGCTGG AGGAGTACGA GCGGCGGCTC
    CTGGTGCAGG AGCAGCAAAT GCAGAAGCTG CTGATGGAGT ACAAGTCCAG GCTGGAGGAC
    AGCGAGGAGA GACTGCGCAG GCAACAGGAG GAGAAGGACA GCCAGATGAA AAGCATTATC
    AGCAGGCTCA TGGCAGTTGA AGAGGAACTG AAGAAAGATC ATGCAGAGAT GCAAGCTGTC
    ATCGATGCAA AGCAGAAAAT TATTGATGCA CAGGAGAAGC GGATCGTGTC CCTGGACTCT
    GCCAACACGC GGCTGATGAG TGCCCTGACG CAGGTGAAGG AGCGCTACAG CATGCAAGTC
    CGCAATGGCA TCTCCCCCAC CAACCCCACC AAGCTTTCCA TCACGGAGAA TGGTGAATTC
    AAAAACAGCA GCTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.