Rasal2 cDNA ORF clone, Mus musculus(house mouse)

The following Rasal2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rasal2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu28681 XM_006496750.2
Latest version!
Mus musculus RAS protein activator like 2 (Rasal2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OMu28682 XM_006496751.2
Latest version!
Mus musculus RAS protein activator like 2 (Rasal2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OMu28683 XM_006496752.2
Latest version!
Mus musculus RAS protein activator like 2 (Rasal2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OMu28684 XM_006496753.4
Latest version!
Mus musculus RAS protein activator like 2 (Rasal2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OMu28686 XM_006496755.3
Latest version!
Mus musculus RAS protein activator like 2 (Rasal2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu28687 XM_006496756.4
Latest version!
Mus musculus RAS protein activator like 2 (Rasal2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu12878 NM_177644.5
Latest version!
Mus musculus RAS protein activator like 2 (Rasal2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OMu28685 XM_006496754.3
Latest version!
Mus musculus RAS protein activator like 2 (Rasal2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
View Old Accession Versions >>
OMu28681 XM_006496750.1 Mus musculus RAS protein activator like 2 (Rasal2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OMu28682 XM_006496751.1 Mus musculus RAS protein activator like 2 (Rasal2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OMu28683 XM_006496752.1 Mus musculus RAS protein activator like 2 (Rasal2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OMu28684 XM_006496753.3 Mus musculus RAS protein activator like 2 (Rasal2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OMu28686 XM_006496755.2 Mus musculus RAS protein activator like 2 (Rasal2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OMu28687 XM_006496756.3 Mus musculus RAS protein activator like 2 (Rasal2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu28681
    Clone ID Related Accession (Same CDS sequence) XM_006496750.1 , XM_006496750.2
    Accession Version XM_006496750.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3870bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product ras GTPase-activating protein nGAP isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006496750.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCTGT CTCCGTCGTC CGGAGGAGCC GCGGAGGCGC TGTCGTGGCC GGAGATGTTC 
    ACGGGGCTGG AGTCCGACTC GCCGCTGCCC CCGGAGGATC TGGACGCGGT CGTCCCAGTC
    AGCGGAGCAG TGGCCGGTGG CATGCTGGAT CGGATCCTTC TGGAGTCAGT GTGCCAGCAG
    CAGAGCTGGG TCCGGGTGTT CGATGTAAAA GGCCCACCAA CCCACCGTCT GTCTTGTGGT
    CAGTCACCCT ATACTGAGAC GACATCATGG GAGAGGAAGT ACTGCATCCT CACAGACAGC
    CAGTTGGTGT TGCTCAACAA GGAGAAGGAG ATGCCTGTGG AAGGACAGGA TCAACAGACA
    GATTCCACCA AAGGGCGCTG TCTGAGGAGA ACTGTCAGTG TTCCTTCAGA GGGTCAGTTT
    CCGGAGTACC CACAGGAGGG CACCACTAAA CTAGAGGTGC CAGCAGAAAG GTCCCCCCGC
    AGACGGAGCA TCTCAGGGAC CAGCACATCA GAGAAACCCA ACTCCATGGA CACTGCGAAT
    ACCTCTCCTT TTAAAGTACC AGGATTCTTC AGCAAGCGCC TGAAAGGTTC CATCAAGAGG
    ACCAAAAGCC AGTCGAAGCT TGACAGAAAC ACAAGCTTTC GGCTTCCATC CCTTCGCAAT
    GCAGATGACA GGTCTCGAGG TCTGCCTAAA CTGAAAGAGT CGCGTTCCCA TGAGTCCCTG
    CTGAGCCCAT GCAGTGCAGT GGAGTGTCTG GATCTAGGTA GAGGAGAGCC AGTCTCAGTG
    AAGCCACTGC ACAGTAGCAT CCTCGGACAG GACTTCTGCT TCGAGGTCAC CTACTTAAGT
    GGAAGTAAAT GCTTTAGCTG TAGCTCTGCC TCCGAGAGAG ATAAGTGGAT GGAAAACCTT
    CGAAGGACCG TTCAGCCAAA TAAGGACAAT TGTAGACGAG CTGAAAATGT TCTCCGTTTA
    TGGATCATTG AAGCCAAGGA CCTTGCCCCT AAAAAAAAAT ATTTCTGCGA ACTGTGCCTT
    GATGATACCC TCTTTGCTCG TACAACCAGC AAGACCAAAG CAGACAATAT TTTTTGGGGT
    GAACATTTTG AATTCTATAG CCTTCCACCT CTTCATAGCA TCACGGTTCA TATTTATAAG
    GATGTGGAAA AAAAGAAAAA AAAGGACAAG AATAATTATG TAGGGCTAGT CAATATCCCC
    ACTGCCAGTG TGACTGGTCG CCAGTTTGTA GAAAAGTGGT ACCCAGTGAG TACACCCACA
    CCCAACAAAG GAAAGACAGG AGGACCTTCT ATTCGGATTA AGTCACGTTT CCAAACTATC
    ACCATTCTGC CTATGGAGCA ATACAAAGAG TTTGCAGAAT TCATCACCAG CAACTACACC
    ATGCTGTGTT CTGTTCTTGA GCCAGTAATT AGTGTGAGGA ATAAAGAGGA GTTGGCTTGT
    GCCTTAGTAC ACATTCTTCA AAGTACTGGC AGAGCCAAGG ATTTTCTGAC TGACTTGGTG
    ATGTCAGAGG TAGATCGTTG TGGAGAACAT GATGTCTTGA TCTTCAGAGA AAACACCATT
    GCCACTAAAT CCATTGAGGA ATACCTCAAG TTGGTGGGAC AACAGTACCT TCATGATGCA
    CTCGGGGAGT TCATCAAAGC CTTGTATGAA TCAGATGAGA ACTGTGAAGT GGATCCCAGC
    AAGTGTTCAT CTAGTGAACT GATGGACCAT CAGAGCAACC TGAAAATGTG CTGTGAGTTG
    GCTTTCTGCA AGATCATCAA CTCATACTGT GTCTTCCCGC GTGAGTTGAA AGAAGTGTTT
    GCATCATGGA AGCAGCAGTG CCTGAACCGA GGCAAGCAAG ACATCAGTGA GCGGCTCATC
    AGTGCCTCAT TGTTTCTCCG TTTTCTTTGC CCAGCCATTA TGTCTCCCAG TCTTTTCAAC
    CTGATGCAGG AATATCCTGA TGACCGCACA TCTCGGACAC TAACTCTTAT TGCCAAGGTC
    ATTCAGAACC TGGCCAACTT TGCCAAGTTT GGTAACAAAG AGGAGTACAT GGCATTCATG
    AATGATTTTT TAGAACATGA ATGGGGTGGA ATGAAGCGGT TCCTTTTGGA GATCTCTAAT
    CCTGACACCA TCTCAAACAC CCCAGGCTTC GATGGTTACA TTGATTTGGG CAGAGAGCTG
    TCAGTTTTGC ATTCCTTACT ATGGGAAGTA GTTTCCCAAC TTGATAAGGG TGAAAATTCC
    TTTCTACAGG CAACTGTGGC AAAACTGGGA CCTCTCCCTC GTGTTCTTGC TGACATTACC
    AAGTCTCTGA CTAATCCTAC GCCAATACAG CAGCAGCTAA GACGTTTCGC TGAGCACAGC
    TCCAGTCCAA ACGTCAGCGG AAGCCTCTCC TCTGGGCTGC AGAGAATATG TGAAGACCCC
    ACTGACAGTG ATTTGCATAA ACTAAAGTCT CCAAGCCAGG ATAACACAGA CAGCTATTTC
    AGAGGAAAGA CGTTATTGTT GGTTCAGCAA GCCTCCTCCC AGAGCATGAC GTACTCTGAA
    AAGGATGAAA AGGAAAACAG CCTTCCTAAC GGTCGGAGCA TCTCCCTCAT GGACCTCCAG
    GACACTCATG CTGCTCAGGC GGAGCATGCA TCTGTCATGC TTGATGTGCC TATGCGCCTT
    GCAGGAAGCC AGCTTTCTAT AACCCAGGTA GCTAGCATCA AACAGCTGCG GGAAACCCAG
    AGCACTCCCC AGAGTGCACC TCAAGTGAGA AGACCCCTGC ACCCAGCCTT GAACCAGCCA
    GGAAGTCTCC AGCCCTTGTC ATTCCAGAAT CCTGTCTATC ACCTCAACAA CCCAGTTCCA
    GCAATGCCAA AGGCCTCTGC GGATTCCAGT TTGGAGAACC TAAGCACTGC CAGTTCTAGA
    AGCCAAAGCA ATAGTGAAGA CTTCAAACTC AGTGGACCCA GCAATAGCAG CATGGAAGAT
    TTCACCAAAA GAAGCTCCCA CAGTGAAGAC TTCTCCAGGC GCCACACTGT ACCAGACAGA
    CACATACCTC TTGCTCTACC ACGACAAAAT AGTACGGGGC AGTCCCAAAT CCGAAAACTG
    GACCATTCTG GGTTAGGTGC CCGAGCCAAA GCACCCCCAT CTCTGCCACA CAGTGCTTCC
    TTGCGTAGCA CAGGGAGCAT GTCAGTGGCG TCTGCAGCCC TAATGGCTGA ACCTGTGCAG
    AACGGGAGCC GGTCCAGGCA GCAGTCCTCT TCCTCCAGAG AGAGCCCTGT TCCCAAAGTG
    AGAGCAATCC AGAGACAACA GACACAACAG GTTCAGTCAC CTGTGGACTC CGCTACAATG
    TCCCCAGTAG AAAGGACAGC AGCCTGGGTT CTGAACAATG GACAGTATGA AGAGGATGTG
    GAAGAAACTG AACAAAATCA AGATGAAGCC AAGCATGCTG AAAAGTATGA ACAAGAAATT
    ACCAAGCTGA AGGAGCGCCT ACGAGTGTCC AGCCGGCGGT TGGAGGAGTA TGAGCGCCGG
    CTGCTGGTGC AGGAGCAGCA GATGCAGAAG CTGCTGCTGG AGTACAAGGC CCGGCTGGAG
    GATAGTGAGG AGCGGCTGCG CAGACAGCAG GAGGAGAAGG ACAGCCAGAT GAAAAGCATC
    ATCAGCAGGC TAATGGCTGT GGAAGAAGAA TTAAAGAAAG ATCATGCTGA GATGCAAGCA
    GTTATTGATG CAAAGCAGAA AATAATTGAT GCACAGGTCA TAAATGGAAA TGAGATTATT
    CAAACAGGAA AAGCGGATCG TGTCCCTGGA TTCAGCCAAC ACGAGACTGA TGAGTGCGCT
    GACACAAGTG AAGGAGCGGT ACAGCATGCA GGTCCGCAAT GGCATCTCTC CCACCAACCC
    CACCAAGCTT TCCATCACGG AGAATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006496813.1
    CDS635..4504
    Translation

    Target ORF information:

    RefSeq Version XM_006496750.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus RAS protein activator like 2 (Rasal2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006496750.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCTGT CTCCGTCGTC CGGAGGAGCC GCGGAGGCGC TGTCGTGGCC GGAGATGTTC 
    ACGGGGCTGG AGTCCGACTC GCCGCTGCCC CCGGAGGATC TGGACGCGGT CGTCCCAGTC
    AGCGGAGCAG TGGCCGGTGG CATGCTGGAT CGGATCCTTC TGGAGTCAGT GTGCCAGCAG
    CAGAGCTGGG TCCGGGTGTT CGATGTAAAA GGCCCACCAA CCCACCGTCT GTCTTGTGGT
    CAGTCACCCT ATACTGAGAC GACATCATGG GAGAGGAAGT ACTGCATCCT CACAGACAGC
    CAGTTGGTGT TGCTCAACAA GGAGAAGGAG ATGCCTGTGG AAGGACAGGA TCAACAGACA
    GATTCCACCA AAGGGCGCTG TCTGAGGAGA ACTGTCAGTG TTCCTTCAGA GGGTCAGTTT
    CCGGAGTACC CACAGGAGGG CACCACTAAA CTAGAGGTGC CAGCAGAAAG GTCCCCCCGC
    AGACGGAGCA TCTCAGGGAC CAGCACATCA GAGAAACCCA ACTCCATGGA CACTGCGAAT
    ACCTCTCCTT TTAAAGTACC AGGATTCTTC AGCAAGCGCC TGAAAGGTTC CATCAAGAGG
    ACCAAAAGCC AGTCGAAGCT TGACAGAAAC ACAAGCTTTC GGCTTCCATC CCTTCGCAAT
    GCAGATGACA GGTCTCGAGG TCTGCCTAAA CTGAAAGAGT CGCGTTCCCA TGAGTCCCTG
    CTGAGCCCAT GCAGTGCAGT GGAGTGTCTG GATCTAGGTA GAGGAGAGCC AGTCTCAGTG
    AAGCCACTGC ACAGTAGCAT CCTCGGACAG GACTTCTGCT TCGAGGTCAC CTACTTAAGT
    GGAAGTAAAT GCTTTAGCTG TAGCTCTGCC TCCGAGAGAG ATAAGTGGAT GGAAAACCTT
    CGAAGGACCG TTCAGCCAAA TAAGGACAAT TGTAGACGAG CTGAAAATGT TCTCCGTTTA
    TGGATCATTG AAGCCAAGGA CCTTGCCCCT AAAAAAAAAT ATTTCTGCGA ACTGTGCCTT
    GATGATACCC TCTTTGCTCG TACAACCAGC AAGACCAAAG CAGACAATAT TTTTTGGGGT
    GAACATTTTG AATTCTATAG CCTTCCACCT CTTCATAGCA TCACGGTTCA TATTTATAAG
    GATGTGGAAA AAAAGAAAAA AAAGGACAAG AATAATTATG TAGGGCTAGT CAATATCCCC
    ACTGCCAGTG TGACTGGTCG CCAGTTTGTA GAAAAGTGGT ACCCAGTGAG TACACCCACA
    CCCAACAAAG GAAAGACAGG AGGACCTTCT ATTCGGATTA AGTCACGTTT CCAAACTATC
    ACCATTCTGC CTATGGAGCA ATACAAAGAG TTTGCAGAAT TCATCACCAG CAACTACACC
    ATGCTGTGTT CTGTTCTTGA GCCAGTAATT AGTGTGAGGA ATAAAGAGGA GTTGGCTTGT
    GCCTTAGTAC ACATTCTTCA AAGTACTGGC AGAGCCAAGG ATTTTCTGAC TGACTTGGTG
    ATGTCAGAGG TAGATCGTTG TGGAGAACAT GATGTCTTGA TCTTCAGAGA AAACACCATT
    GCCACTAAAT CCATTGAGGA ATACCTCAAG TTGGTGGGAC AACAGTACCT TCATGATGCA
    CTCGGGGAGT TCATCAAAGC CTTGTATGAA TCAGATGAGA ACTGTGAAGT GGATCCCAGC
    AAGTGTTCAT CTAGTGAACT GATGGACCAT CAGAGCAACC TGAAAATGTG CTGTGAGTTG
    GCTTTCTGCA AGATCATCAA CTCATACTGT GTCTTCCCGC GTGAGTTGAA AGAAGTGTTT
    GCATCATGGA AGCAGCAGTG CCTGAACCGA GGCAAGCAAG ACATCAGTGA GCGGCTCATC
    AGTGCCTCAT TGTTTCTCCG TTTTCTTTGC CCAGCCATTA TGTCTCCCAG TCTTTTCAAC
    CTGATGCAGG AATATCCTGA TGACCGCACA TCTCGGACAC TAACTCTTAT TGCCAAGGTC
    ATTCAGAACC TGGCCAACTT TGCCAAGTTT GGTAACAAAG AGGAGTACAT GGCATTCATG
    AATGATTTTT TAGAACATGA ATGGGGTGGA ATGAAGCGGT TCCTTTTGGA GATCTCTAAT
    CCTGACACCA TCTCAAACAC CCCAGGCTTC GATGGTTACA TTGATTTGGG CAGAGAGCTG
    TCAGTTTTGC ATTCCTTACT ATGGGAAGTA GTTTCCCAAC TTGATAAGGG TGAAAATTCC
    TTTCTACAGG CAACTGTGGC AAAACTGGGA CCTCTCCCTC GTGTTCTTGC TGACATTACC
    AAGTCTCTGA CTAATCCTAC GCCAATACAG CAGCAGCTAA GACGTTTCGC TGAGCACAGC
    TCCAGTCCAA ACGTCAGCGG AAGCCTCTCC TCTGGGCTGC AGAGAATATG TGAAGACCCC
    ACTGACAGTG ATTTGCATAA ACTAAAGTCT CCAAGCCAGG ATAACACAGA CAGCTATTTC
    AGAGGAAAGA CGTTATTGTT GGTTCAGCAA GCCTCCTCCC AGAGCATGAC GTACTCTGAA
    AAGGATGAAA AGGAAAACAG CCTTCCTAAC GGTCGGAGCA TCTCCCTCAT GGACCTCCAG
    GACACTCATG CTGCTCAGGC GGAGCATGCA TCTGTCATGC TTGATGTGCC TATGCGCCTT
    GCAGGAAGCC AGCTTTCTAT AACCCAGGTA GCTAGCATCA AACAGCTGCG GGAAACCCAG
    AGCACTCCCC AGAGTGCACC TCAAGTGAGA AGACCCCTGC ACCCAGCCTT GAACCAGCCA
    GGAAGTCTCC AGCCCTTGTC ATTCCAGAAT CCTGTCTATC ACCTCAACAA CCCAGTTCCA
    GCAATGCCAA AGGCCTCTGC GGATTCCAGT TTGGAGAACC TAAGCACTGC CAGTTCTAGA
    AGCCAAAGCA ATAGTGAAGA CTTCAAACTC AGTGGACCCA GCAATAGCAG CATGGAAGAT
    TTCACCAAAA GAAGCTCCCA CAGTGAAGAC TTCTCCAGGC GCCACACTGT ACCAGACAGA
    CACATACCTC TTGCTCTACC ACGACAAAAT AGTACGGGGC AGTCCCAAAT CCGAAAACTG
    GACCATTCTG GGTTAGGTGC CCGAGCCAAA GCACCCCCAT CTCTGCCACA CAGTGCTTCC
    TTGCGTAGCA CAGGGAGCAT GTCAGTGGCG TCTGCAGCCC TAATGGCTGA ACCTGTGCAG
    AACGGGAGCC GGTCCAGGCA GCAGTCCTCT TCCTCCAGAG AGAGCCCTGT TCCCAAAGTG
    AGAGCAATCC AGAGACAACA GACACAACAG GTTCAGTCAC CTGTGGACTC CGCTACAATG
    TCCCCAGTAG AAAGGACAGC AGCCTGGGTT CTGAACAATG GACAGTATGA AGAGGATGTG
    GAAGAAACTG AACAAAATCA AGATGAAGCC AAGCATGCTG AAAAGTATGA ACAAGAAATT
    ACCAAGCTGA AGGAGCGCCT ACGAGTGTCC AGCCGGCGGT TGGAGGAGTA TGAGCGCCGG
    CTGCTGGTGC AGGAGCAGCA GATGCAGAAG CTGCTGCTGG AGTACAAGGC CCGGCTGGAG
    GATAGTGAGG AGCGGCTGCG CAGACAGCAG GAGGAGAAGG ACAGCCAGAT GAAAAGCATC
    ATCAGCAGGC TAATGGCTGT GGAAGAAGAA TTAAAGAAAG ATCATGCTGA GATGCAAGCA
    GTTATTGATG CAAAGCAGAA AATAATTGAT GCACAGGTCA TAAATGGAAA TGAGATTATT
    CAAACAGGAA AAGCGGATCG TGTCCCTGGA TTCAGCCAAC ACGAGACTGA TGAGTGCGCT
    GACACAAGTG AAGGAGCGGT ACAGCATGCA GGTCCGCAAT GGCATCTCTC CCACCAACCC
    CACCAAGCTT TCCATCACGG AGAATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu28682
    Clone ID Related Accession (Same CDS sequence) XM_006496751.2 , XM_006496751.1
    Accession Version XM_006496751.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3849bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product ras GTPase-activating protein nGAP isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006496751.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCTGT CTCCGTCGTC CGGAGGAGCC GCGGAGGCGC TGTCGTGGCC GGAGATGTTC 
    ACGGGGCTGG AGTCCGACTC GCCGCTGCCC CCGGAGGATC TGGACGCGGT CGTCCCAGTC
    AGCGGAGCAG TGGCCGGTGG CATGCTGGAT CGGATCCTTC TGGAGTCAGT GTGCCAGCAG
    CAGAGCTGGG TCCGGGTGTT CGATGTAAAA GGCCCACCAA CCCACCGTCT GTCTTGTGGT
    CAGTCACCCT ATACTGAGAC GACATCATGG GAGAGGAAGT ACTGCATCCT CACAGACAGC
    CAGTTGGTGT TGCTCAACAA GGAGAAGGAG ATGCCTGTGG AAGGACAGGA TCAACAGACA
    GATTCCACCA AAGGGCGCTG TCTGAGGAGA ACTGTCAGTG TTCCTTCAGA GGGTCAGTTT
    CCGGAGTACC CACAGGAGGG CACCACTAAA CTAGAGGTGC CAGCAGAAAG GTCCCCCCGC
    AGACGGAGCA TCTCAGGGAC CAGCACATCA GAGAAACCCA ACTCCATGGA CACTGCGAAT
    ACCTCTCCTT TTAAAGTACC AGGATTCTTC AGCAAGCGCC TGAAAGGTTC CATCAAGAGG
    ACCAAAAGCC AGTCGAAGCT TGACAGAAAC ACAAGCTTTC GGCTTCCATC CCTTCGCAAT
    GCAGATGACA GGTCTCGAGG TCTGCCTAAA CTGAAAGAGT CGCGTTCCCA TGAGTCCCTG
    CTGAGCCCAT GCAGTGCAGT GGAGTGTCTG GATCTAGGTA GAGGAGAGCC AGTCTCAGTG
    AAGCCACTGC ACAGTAGCAT CCTCGGACAG GACTTCTGCT TCGAGGTCAC CTACTTAAGT
    GGAAGTAAAT GCTTTAGCTG TAGCTCTGCC TCCGAGAGAG ATAAGTGGAT GGAAAACCTT
    CGAAGGACCG TTCAGCCAAA TAAGGACAAT TGTAGACGAG CTGAAAATGT TCTCCGTTTA
    TGGATCATTG AAGCCAAGGA CCTTGCCCCT AAAAAAAAAT ATTTCTGCGA ACTGTGCCTT
    GATGATACCC TCTTTGCTCG TACAACCAGC AAGACCAAAG CAGACAATAT TTTTTGGGGT
    GAACATTTTG AATTCTATAG CCTTCCACCT CTTCATAGCA TCACGGTTCA TATTTATAAG
    GATGTGGAAA AAAAGAAAAA AAAGGACAAG AATAATTATG TAGGGCTAGT CAATATCCCC
    ACTGCCAGTG TGACTGGTCG CCAGTTTGTA GAAAAGTGGT ACCCAGTGAG TACACCCACA
    CCCAACAAAG GAAAGACAGG AGGACCTTCT ATTCGGATTA AGTCACGTTT CCAAACTATC
    ACCATTCTGC CTATGGAGCA ATACAAAGAG TTTGCAGAAT TCATCACCAG CAACTACACC
    ATGCTGTGTT CTGTTCTTGA GCCAGTAATT AGTGTGAGGA ATAAAGAGGA GTTGGCTTGT
    GCCTTAGTAC ACATTCTTCA AAGTACTGGC AGAGCCAAGG ATTTTCTGAC TGACTTGGTG
    ATGTCAGAGG TAGATCGTTG TGGAGAACAT GATGTCTTGA TCTTCAGAGA AAACACCATT
    GCCACTAAAT CCATTGAGGA ATACCTCAAG TTGGTGGGAC AACAGTACCT TCATGATGCA
    CTCGGGGAGT TCATCAAAGC CTTGTATGAA TCAGATGAGA ACTGTGAAGT GGATCCCAGC
    AAGTGTTCAT CTAGTGAACT GATGGACCAT CAGAGCAACC TGAAAATGTG CTGTGAGTTG
    GCTTTCTGCA AGATCATCAA CTCATACTGT GTCTTCCCGC GTGAGTTGAA AGAAGTGTTT
    GCATCATGGA AGCAGCAGTG CCTGAACCGA GGCAAGCAAG ACATCAGTGA GCGGCTCATC
    AGTGCCTCAT TGTTTCTCCG TTTTCTTTGC CCAGCCATTA TGTCTCCCAG TCTTTTCAAC
    CTGATGCAGG AATATCCTGA TGACCGCACA TCTCGGACAC TAACTCTTAT TGCCAAGGTC
    ATTCAGAACC TGGCCAACTT TGCCAAGTTT GGTAACAAAG AGGAGTACAT GGCATTCATG
    AATGATTTTT TAGAACATGA ATGGGGTGGA ATGAAGCGGT TCCTTTTGGA GATCTCTAAT
    CCTGACACCA TCTCAAACAC CCCAGGCTTC GATGGTTACA TTGATTTGGG CAGAGAGCTG
    TCAGTTTTGC ATTCCTTACT ATGGGAAGTA GTTTCCCAAC TTGATAAGGC AACTGTGGCA
    AAACTGGGAC CTCTCCCTCG TGTTCTTGCT GACATTACCA AGTCTCTGAC TAATCCTACG
    CCAATACAGC AGCAGCTAAG ACGTTTCGCT GAGCACAGCT CCAGTCCAAA CGTCAGCGGA
    AGCCTCTCCT CTGGGCTGCA GAGAATATGT GAAGACCCCA CTGACAGTGA TTTGCATAAA
    CTAAAGTCTC CAAGCCAGGA TAACACAGAC AGCTATTTCA GAGGAAAGAC GTTATTGTTG
    GTTCAGCAAG CCTCCTCCCA GAGCATGACG TACTCTGAAA AGGATGAAAA GGAAAACAGC
    CTTCCTAACG GTCGGAGCAT CTCCCTCATG GACCTCCAGG ACACTCATGC TGCTCAGGCG
    GAGCATGCAT CTGTCATGCT TGATGTGCCT ATGCGCCTTG CAGGAAGCCA GCTTTCTATA
    ACCCAGGTAG CTAGCATCAA ACAGCTGCGG GAAACCCAGA GCACTCCCCA GAGTGCACCT
    CAAGTGAGAA GACCCCTGCA CCCAGCCTTG AACCAGCCAG GAAGTCTCCA GCCCTTGTCA
    TTCCAGAATC CTGTCTATCA CCTCAACAAC CCAGTTCCAG CAATGCCAAA GGCCTCTGCG
    GATTCCAGTT TGGAGAACCT AAGCACTGCC AGTTCTAGAA GCCAAAGCAA TAGTGAAGAC
    TTCAAACTCA GTGGACCCAG CAATAGCAGC ATGGAAGATT TCACCAAAAG AAGCTCCCAC
    AGTGAAGACT TCTCCAGGCG CCACACTGTA CCAGACAGAC ACATACCTCT TGCTCTACCA
    CGACAAAATA GTACGGGGCA GTCCCAAATC CGAAAACTGG ACCATTCTGG GTTAGGTGCC
    CGAGCCAAAG CACCCCCATC TCTGCCACAC AGTGCTTCCT TGCGTAGCAC AGGGAGCATG
    TCAGTGGCGT CTGCAGCCCT AATGGCTGAA CCTGTGCAGA ACGGGAGCCG GTCCAGGCAG
    CAGTCCTCTT CCTCCAGAGA GAGCCCTGTT CCCAAAGTGA GAGCAATCCA GAGACAACAG
    ACACAACAGG TTCAGTCACC TGTGGACTCC GCTACAATGT CCCCAGTAGA AAGGACAGCA
    GCCTGGGTTC TGAACAATGG ACAGTATGAA GAGGATGTGG AAGAAACTGA ACAAAATCAA
    GATGAAGCCA AGCATGCTGA AAAGTATGAA CAAGAAATTA CCAAGCTGAA GGAGCGCCTA
    CGAGTGTCCA GCCGGCGGTT GGAGGAGTAT GAGCGCCGGC TGCTGGTGCA GGAGCAGCAG
    ATGCAGAAGC TGCTGCTGGA GTACAAGGCC CGGCTGGAGG ATAGTGAGGA GCGGCTGCGC
    AGACAGCAGG AGGAGAAGGA CAGCCAGATG AAAAGCATCA TCAGCAGGCT AATGGCTGTG
    GAAGAAGAAT TAAAGAAAGA TCATGCTGAG ATGCAAGCAG TTATTGATGC AAAGCAGAAA
    ATAATTGATG CACAGGTCAT AAATGGAAAT GAGATTATTC AAACAGGAAA AGCGGATCGT
    GTCCCTGGAT TCAGCCAACA CGAGACTGAT GAGTGCGCTG ACACAAGTGA AGGAGCGGTA
    CAGCATGCAG GTCCGCAATG GCATCTCTCC CACCAACCCC ACCAAGCTTT CCATCACGGA
    GAATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006496814.1
    CDS635..4483
    Translation

    Target ORF information:

    RefSeq Version XM_006496751.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus RAS protein activator like 2 (Rasal2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006496751.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCTGT CTCCGTCGTC CGGAGGAGCC GCGGAGGCGC TGTCGTGGCC GGAGATGTTC 
    ACGGGGCTGG AGTCCGACTC GCCGCTGCCC CCGGAGGATC TGGACGCGGT CGTCCCAGTC
    AGCGGAGCAG TGGCCGGTGG CATGCTGGAT CGGATCCTTC TGGAGTCAGT GTGCCAGCAG
    CAGAGCTGGG TCCGGGTGTT CGATGTAAAA GGCCCACCAA CCCACCGTCT GTCTTGTGGT
    CAGTCACCCT ATACTGAGAC GACATCATGG GAGAGGAAGT ACTGCATCCT CACAGACAGC
    CAGTTGGTGT TGCTCAACAA GGAGAAGGAG ATGCCTGTGG AAGGACAGGA TCAACAGACA
    GATTCCACCA AAGGGCGCTG TCTGAGGAGA ACTGTCAGTG TTCCTTCAGA GGGTCAGTTT
    CCGGAGTACC CACAGGAGGG CACCACTAAA CTAGAGGTGC CAGCAGAAAG GTCCCCCCGC
    AGACGGAGCA TCTCAGGGAC CAGCACATCA GAGAAACCCA ACTCCATGGA CACTGCGAAT
    ACCTCTCCTT TTAAAGTACC AGGATTCTTC AGCAAGCGCC TGAAAGGTTC CATCAAGAGG
    ACCAAAAGCC AGTCGAAGCT TGACAGAAAC ACAAGCTTTC GGCTTCCATC CCTTCGCAAT
    GCAGATGACA GGTCTCGAGG TCTGCCTAAA CTGAAAGAGT CGCGTTCCCA TGAGTCCCTG
    CTGAGCCCAT GCAGTGCAGT GGAGTGTCTG GATCTAGGTA GAGGAGAGCC AGTCTCAGTG
    AAGCCACTGC ACAGTAGCAT CCTCGGACAG GACTTCTGCT TCGAGGTCAC CTACTTAAGT
    GGAAGTAAAT GCTTTAGCTG TAGCTCTGCC TCCGAGAGAG ATAAGTGGAT GGAAAACCTT
    CGAAGGACCG TTCAGCCAAA TAAGGACAAT TGTAGACGAG CTGAAAATGT TCTCCGTTTA
    TGGATCATTG AAGCCAAGGA CCTTGCCCCT AAAAAAAAAT ATTTCTGCGA ACTGTGCCTT
    GATGATACCC TCTTTGCTCG TACAACCAGC AAGACCAAAG CAGACAATAT TTTTTGGGGT
    GAACATTTTG AATTCTATAG CCTTCCACCT CTTCATAGCA TCACGGTTCA TATTTATAAG
    GATGTGGAAA AAAAGAAAAA AAAGGACAAG AATAATTATG TAGGGCTAGT CAATATCCCC
    ACTGCCAGTG TGACTGGTCG CCAGTTTGTA GAAAAGTGGT ACCCAGTGAG TACACCCACA
    CCCAACAAAG GAAAGACAGG AGGACCTTCT ATTCGGATTA AGTCACGTTT CCAAACTATC
    ACCATTCTGC CTATGGAGCA ATACAAAGAG TTTGCAGAAT TCATCACCAG CAACTACACC
    ATGCTGTGTT CTGTTCTTGA GCCAGTAATT AGTGTGAGGA ATAAAGAGGA GTTGGCTTGT
    GCCTTAGTAC ACATTCTTCA AAGTACTGGC AGAGCCAAGG ATTTTCTGAC TGACTTGGTG
    ATGTCAGAGG TAGATCGTTG TGGAGAACAT GATGTCTTGA TCTTCAGAGA AAACACCATT
    GCCACTAAAT CCATTGAGGA ATACCTCAAG TTGGTGGGAC AACAGTACCT TCATGATGCA
    CTCGGGGAGT TCATCAAAGC CTTGTATGAA TCAGATGAGA ACTGTGAAGT GGATCCCAGC
    AAGTGTTCAT CTAGTGAACT GATGGACCAT CAGAGCAACC TGAAAATGTG CTGTGAGTTG
    GCTTTCTGCA AGATCATCAA CTCATACTGT GTCTTCCCGC GTGAGTTGAA AGAAGTGTTT
    GCATCATGGA AGCAGCAGTG CCTGAACCGA GGCAAGCAAG ACATCAGTGA GCGGCTCATC
    AGTGCCTCAT TGTTTCTCCG TTTTCTTTGC CCAGCCATTA TGTCTCCCAG TCTTTTCAAC
    CTGATGCAGG AATATCCTGA TGACCGCACA TCTCGGACAC TAACTCTTAT TGCCAAGGTC
    ATTCAGAACC TGGCCAACTT TGCCAAGTTT GGTAACAAAG AGGAGTACAT GGCATTCATG
    AATGATTTTT TAGAACATGA ATGGGGTGGA ATGAAGCGGT TCCTTTTGGA GATCTCTAAT
    CCTGACACCA TCTCAAACAC CCCAGGCTTC GATGGTTACA TTGATTTGGG CAGAGAGCTG
    TCAGTTTTGC ATTCCTTACT ATGGGAAGTA GTTTCCCAAC TTGATAAGGC AACTGTGGCA
    AAACTGGGAC CTCTCCCTCG TGTTCTTGCT GACATTACCA AGTCTCTGAC TAATCCTACG
    CCAATACAGC AGCAGCTAAG ACGTTTCGCT GAGCACAGCT CCAGTCCAAA CGTCAGCGGA
    AGCCTCTCCT CTGGGCTGCA GAGAATATGT GAAGACCCCA CTGACAGTGA TTTGCATAAA
    CTAAAGTCTC CAAGCCAGGA TAACACAGAC AGCTATTTCA GAGGAAAGAC GTTATTGTTG
    GTTCAGCAAG CCTCCTCCCA GAGCATGACG TACTCTGAAA AGGATGAAAA GGAAAACAGC
    CTTCCTAACG GTCGGAGCAT CTCCCTCATG GACCTCCAGG ACACTCATGC TGCTCAGGCG
    GAGCATGCAT CTGTCATGCT TGATGTGCCT ATGCGCCTTG CAGGAAGCCA GCTTTCTATA
    ACCCAGGTAG CTAGCATCAA ACAGCTGCGG GAAACCCAGA GCACTCCCCA GAGTGCACCT
    CAAGTGAGAA GACCCCTGCA CCCAGCCTTG AACCAGCCAG GAAGTCTCCA GCCCTTGTCA
    TTCCAGAATC CTGTCTATCA CCTCAACAAC CCAGTTCCAG CAATGCCAAA GGCCTCTGCG
    GATTCCAGTT TGGAGAACCT AAGCACTGCC AGTTCTAGAA GCCAAAGCAA TAGTGAAGAC
    TTCAAACTCA GTGGACCCAG CAATAGCAGC ATGGAAGATT TCACCAAAAG AAGCTCCCAC
    AGTGAAGACT TCTCCAGGCG CCACACTGTA CCAGACAGAC ACATACCTCT TGCTCTACCA
    CGACAAAATA GTACGGGGCA GTCCCAAATC CGAAAACTGG ACCATTCTGG GTTAGGTGCC
    CGAGCCAAAG CACCCCCATC TCTGCCACAC AGTGCTTCCT TGCGTAGCAC AGGGAGCATG
    TCAGTGGCGT CTGCAGCCCT AATGGCTGAA CCTGTGCAGA ACGGGAGCCG GTCCAGGCAG
    CAGTCCTCTT CCTCCAGAGA GAGCCCTGTT CCCAAAGTGA GAGCAATCCA GAGACAACAG
    ACACAACAGG TTCAGTCACC TGTGGACTCC GCTACAATGT CCCCAGTAGA AAGGACAGCA
    GCCTGGGTTC TGAACAATGG ACAGTATGAA GAGGATGTGG AAGAAACTGA ACAAAATCAA
    GATGAAGCCA AGCATGCTGA AAAGTATGAA CAAGAAATTA CCAAGCTGAA GGAGCGCCTA
    CGAGTGTCCA GCCGGCGGTT GGAGGAGTAT GAGCGCCGGC TGCTGGTGCA GGAGCAGCAG
    ATGCAGAAGC TGCTGCTGGA GTACAAGGCC CGGCTGGAGG ATAGTGAGGA GCGGCTGCGC
    AGACAGCAGG AGGAGAAGGA CAGCCAGATG AAAAGCATCA TCAGCAGGCT AATGGCTGTG
    GAAGAAGAAT TAAAGAAAGA TCATGCTGAG ATGCAAGCAG TTATTGATGC AAAGCAGAAA
    ATAATTGATG CACAGGTCAT AAATGGAAAT GAGATTATTC AAACAGGAAA AGCGGATCGT
    GTCCCTGGAT TCAGCCAACA CGAGACTGAT GAGTGCGCTG ACACAAGTGA AGGAGCGGTA
    CAGCATGCAG GTCCGCAATG GCATCTCTCC CACCAACCCC ACCAAGCTTT CCATCACGGA
    GAATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu28683
    Clone ID Related Accession (Same CDS sequence) XM_006496752.1 , XM_006496752.2
    Accession Version XM_006496752.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3840bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product ras GTPase-activating protein nGAP isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006496752.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGCTGT CTCCGTCGTC CGGAGGAGCC GCGGAGGCGC TGTCGTGGCC GGAGATGTTC 
    ACGGGGCTGG AGTCCGACTC GCCGCTGCCC CCGGAGGATC TGGACGCGGT CGTCCCAGTC
    AGCGGAGCAG TGGCCGGTGG CATGCTGGAT CGGATCCTTC TGGAGTCAGT GTGCCAGCAG
    CAGAGCTGGG TCCGGGTGTT CGATGTAAAA GGCCCACCAA CCCACCGTCT GTCTTGTGGT
    CAGTCACCCT ATACTGAGAC GACATCATGG GAGAGGAAGT ACTGCATCCT CACAGACAGC
    CAGTTGGTGT TGCTCAACAA GGAGAAGGAG ATGCCTGTGG AAGGACAGGA TCAACAGACA
    GATTCCACCA AAGGGCGCTG TCTGAGGAGA ACTGTCAGTG TTCCTTCAGA GGGTCAGTTT
    CCGGAGTACC CACAGGAGGG CACCACTAAA CTAGAGGTGC CAGCAGAAAG GTCCCCCCGC
    AGACGGAGCA TCTCAGGGAC CAGCACATCA GAGAAACCCA ACTCCATGGA CACTGCGAAT
    ACCTCTCCTT TTAAAGTACC AGGATTCTTC AGCAAGCGCC TGAAAGGTTC CATCAAGAGG
    ACCAAAAGCC AGTCGAAGCT TGACAGAAAC ACAAGCTTTC GGCTTCCATC CCTTCGCAAT
    GCAGATGACA GGTCTCGAGG TCTGCCTAAA CTGAAAGAGT CGCGTTCCCA TGAGTCCCTG
    CTGAGCCCAT GCAGTGCAGT GGAGTGTCTG GATCTAGGTA GAGGAGAGCC AGTCTCAGTG
    AAGCCACTGC ACAGTAGCAT CCTCGGACAG GACTTCTGCT TCGAGGTCAC CTACTTAAGT
    GGAAGTAAAT GCTTTAGCTG TAGCTCTGCC TCCGAGAGAG ATAAGTGGAT GGAAAACCTT
    CGAAGGACCG TTCAGCCAAA TAAGGACAAT TGTAGACGAG CTGAAAATGT TCTCCGTTTA
    TGGATCATTG AAGCCAAGGA CCTTGCCCCT AAAAAAAAAT ATTTCTGCGA ACTGTGCCTT
    GATGATACCC TCTTTGCTCG TACAACCAGC AAGACCAAAG CAGACAATAT TTTTTGGGGT
    GAACATTTTG AATTCTATAG CCTTCCACCT CTTCATAGCA TCACGGTTCA TATTTATAAG
    GATGTGGAAA AAAAGAAAAA AAAGGACAAG AATAATTATG TAGGGCTAGT CAATATCCCC
    ACTGCCAGTG TGACTGGTCG CCAGTTTGTA GAAAAGTGGT ACCCAGTGAG TACACCCACA
    CCCAACAAAG GAAAGACAGG AGGACCTTCT ATTCGGATTA AGTCACGTTT CCAAACTATC
    ACCATTCTGC CTATGGAGCA ATACAAAGAG TTTGCAGAAT TCATCACCAG CAACTACACC
    ATGCTGTGTT CTGTTCTTGA GCCAGTAATT AGTGTGAGGA ATAAAGAGGA GTTGGCTTGT
    GCCTTAGTAC ACATTCTTCA AAGTACTGGC AGAGCCAAGG ATTTTCTGAC TGACTTGGTG
    ATGTCAGAGG TAGATCGTTG TGGAGAACAT GATGTCTTGA TCTTCAGAGA AAACACCATT
    GCCACTAAAT CCATTGAGGA ATACCTCAAG TTGGTGGGAC AACAGTACCT TCATGATGCA
    CTCGGGGAGT TCATCAAAGC CTTGTATGAA TCAGATGAGA ACTGTGAAGT GGATCCCAGC
    AAGTGTTCAT CTAGTGAACT GATGGACCAT CAGAGCAACC TGAAAATGTG CTGTGAGTTG
    GCTTTCTGCA AGATCATCAA CTCATACTGT GTCTTCCCGC GTGAGTTGAA AGAAGTGTTT
    GCATCATGGA AGCAGCAGTG CCTGAACCGA GGCAAGCAAG ACATCAGTGA GCGGCTCATC
    AGTGCCTCAT TGTTTCTCCG TTTTCTTTGC CCAGCCATTA TGTCTCCCAG TCTTTTCAAC
    CTGATGCAGG AATATCCTGA TGACCGCACA TCTCGGACAC TAACTCTTAT TGCCAAGGTC
    ATTCAGAACC TGGCCAACTT TGCCAAGTTT GGTAACAAAG AGGAGTACAT GGCATTCATG
    AATGATTTTT TAGAACATGA ATGGGGTGGA ATGAAGCGGT TCCTTTTGGA GATCTCTAAT
    CCTGACACCA TCTCAAACAC CCCAGGCTTC GATGGTTACA TTGATTTGGG CAGAGAGCTG
    TCAGTTTTGC ATTCCTTACT ATGGGAAGTA GTTTCCCAAC TTGATAAGGC AACTGTGGCA
    AAACTGGGAC CTCTCCCTCG TGTTCTTGCT GACATTACCA AGTCTCTGAC TAATCCTACG
    CCAATACAGC AGCAGCTAAG ACGTTTCGCT GAGCACAGCT CCAGTCCAAA CGTCAGCGGA
    AGCCTCTCCT CTGGGCTGCA GAGAATATGT GAAGACCCCA CTGACAGTGA TTTGCATAAA
    CTAAAGTCTC CAAGCCAGGA TAACACAGAC AGCTATTTCA GAGGAAAGAC GTTATTGTTG
    GTTCAGCAAG CCTCCTCCCA GAGCATGACG TACTCTGAAA AGGATGAAAA GGAAAACAGC
    CTTCCTAACG GTCGGAGCAT CTCCCTCATG GACCTCCAGG ACACTCATGC TGCTCAGGCG
    GAGCATGCAT CTGTCATGCT TGATGTGCCT ATGCGCCTTG CAGGAAGCCA GCTTTCTATA
    ACCCAGGTAG CTAGCATCAA ACAGCTGCGG GAAACCCAGA GCACTCCCCA GAGTGCACCT
    CAAGTGAGAA GACCCCTGCA CCCAGCCTTG AACCAGCCAG GAAGTCTCCA GCCCTTGTCA
    TTCCAGAATC CTGTCTATCA CCTCAACAAC CCAGTTCCAG CAATGCCAAA GGCCTCTGCG
    GATTCCAGTT TGGAGAACCT AAGCACTGCC AGTTCTAGAA GCCAAAGCAA TAGTGAAGAC
    TTCAAACTCA GTGGACCCAG CAATAGCAGC ATGGAAGATT TCACCAAAAG AAGCTCCCAC
    AGTGAAGACT TCTCCAGGCG CCACACTGTA CCAGACAGAC ACATACCTCT TGCTCTACCA
    CGACAAAATA GTACGGGGCA GTCCCAAATC CGAAAACTGG ACCATTCTGG GTTAGGTGCC
    CGAGCCAAAG CACCCCCATC TCTGCCACAC AGTGCTTCCT TGCGTAGCAC AGGGAGCATG
    TCAGTGGCGT CTGCAGCCCT AATGGCTGAA CCTGTGCAGA ACGGGAGCCG GTCCAGGCAG
    CAGTCCTCTT CCTCCAGAGA GAGCCCTGTT CCCAAAGTGA GAGCAATCCA GAGACAACAG
    ACACAACAGG TTCAGTCACC TGTGGACTCC GCTACAATGT CCCCAGTAGA AAGGACAGCA
    GCCTGGGTTC TGAACAATGG ACAGTATGAA GAGGATGTGG AAGAAACTGA ACAAAATCAA
    GATGAAGCCA AGCATGCTGA AAAGTATGAA CAAGAAATTA CCAAGCTGAA GGAGCGCCTA
    CGAGTGTCCA GCCGGCGGTT GGAGGAGTAT GAGCGCCGGC TGCTGGTGCA GGAGCAGCAG
    ATGCAGAAGC TGCTGCTGGA GTACAAGGCC CGGCTGGAGG ATAGTGAGGA GCGGCTGCGC
    AGACAGCAGG AGGAGAAGGA CAGCCAGATG AAAAGCATCA TCAGCAGGCT AATGGCTGTG
    GAAGAAGAAT TAAAGAAAGA TCATGCTGAG ATGCAAGCAG TTATTGATGC AAAGCAGAAA
    ATAATTGATG CACAGGAAAA GCGGATCGTG TCCCTGGATT CAGCCAACAC GAGACTGATG
    AGTGCGCTGA CACAAGTGAA GGAGCGGTAC AGCATGCAGG TCCGCAATGG CATCTCTCCC
    ACCAACCCCA CCAAGCTTTC CATCACGGAG AATGGTGAAT TCAAAAATAG CAGCTGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006496815.1
    CDS635..4474
    Translation

    Target ORF information:

    RefSeq Version XM_006496752.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus RAS protein activator like 2 (Rasal2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006496752.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGCTGT CTCCGTCGTC CGGAGGAGCC GCGGAGGCGC TGTCGTGGCC GGAGATGTTC 
    ACGGGGCTGG AGTCCGACTC GCCGCTGCCC CCGGAGGATC TGGACGCGGT CGTCCCAGTC
    AGCGGAGCAG TGGCCGGTGG CATGCTGGAT CGGATCCTTC TGGAGTCAGT GTGCCAGCAG
    CAGAGCTGGG TCCGGGTGTT CGATGTAAAA GGCCCACCAA CCCACCGTCT GTCTTGTGGT
    CAGTCACCCT ATACTGAGAC GACATCATGG GAGAGGAAGT ACTGCATCCT CACAGACAGC
    CAGTTGGTGT TGCTCAACAA GGAGAAGGAG ATGCCTGTGG AAGGACAGGA TCAACAGACA
    GATTCCACCA AAGGGCGCTG TCTGAGGAGA ACTGTCAGTG TTCCTTCAGA GGGTCAGTTT
    CCGGAGTACC CACAGGAGGG CACCACTAAA CTAGAGGTGC CAGCAGAAAG GTCCCCCCGC
    AGACGGAGCA TCTCAGGGAC CAGCACATCA GAGAAACCCA ACTCCATGGA CACTGCGAAT
    ACCTCTCCTT TTAAAGTACC AGGATTCTTC AGCAAGCGCC TGAAAGGTTC CATCAAGAGG
    ACCAAAAGCC AGTCGAAGCT TGACAGAAAC ACAAGCTTTC GGCTTCCATC CCTTCGCAAT
    GCAGATGACA GGTCTCGAGG TCTGCCTAAA CTGAAAGAGT CGCGTTCCCA TGAGTCCCTG
    CTGAGCCCAT GCAGTGCAGT GGAGTGTCTG GATCTAGGTA GAGGAGAGCC AGTCTCAGTG
    AAGCCACTGC ACAGTAGCAT CCTCGGACAG GACTTCTGCT TCGAGGTCAC CTACTTAAGT
    GGAAGTAAAT GCTTTAGCTG TAGCTCTGCC TCCGAGAGAG ATAAGTGGAT GGAAAACCTT
    CGAAGGACCG TTCAGCCAAA TAAGGACAAT TGTAGACGAG CTGAAAATGT TCTCCGTTTA
    TGGATCATTG AAGCCAAGGA CCTTGCCCCT AAAAAAAAAT ATTTCTGCGA ACTGTGCCTT
    GATGATACCC TCTTTGCTCG TACAACCAGC AAGACCAAAG CAGACAATAT TTTTTGGGGT
    GAACATTTTG AATTCTATAG CCTTCCACCT CTTCATAGCA TCACGGTTCA TATTTATAAG
    GATGTGGAAA AAAAGAAAAA AAAGGACAAG AATAATTATG TAGGGCTAGT CAATATCCCC
    ACTGCCAGTG TGACTGGTCG CCAGTTTGTA GAAAAGTGGT ACCCAGTGAG TACACCCACA
    CCCAACAAAG GAAAGACAGG AGGACCTTCT ATTCGGATTA AGTCACGTTT CCAAACTATC
    ACCATTCTGC CTATGGAGCA ATACAAAGAG TTTGCAGAAT TCATCACCAG CAACTACACC
    ATGCTGTGTT CTGTTCTTGA GCCAGTAATT AGTGTGAGGA ATAAAGAGGA GTTGGCTTGT
    GCCTTAGTAC ACATTCTTCA AAGTACTGGC AGAGCCAAGG ATTTTCTGAC TGACTTGGTG
    ATGTCAGAGG TAGATCGTTG TGGAGAACAT GATGTCTTGA TCTTCAGAGA AAACACCATT
    GCCACTAAAT CCATTGAGGA ATACCTCAAG TTGGTGGGAC AACAGTACCT TCATGATGCA
    CTCGGGGAGT TCATCAAAGC CTTGTATGAA TCAGATGAGA ACTGTGAAGT GGATCCCAGC
    AAGTGTTCAT CTAGTGAACT GATGGACCAT CAGAGCAACC TGAAAATGTG CTGTGAGTTG
    GCTTTCTGCA AGATCATCAA CTCATACTGT GTCTTCCCGC GTGAGTTGAA AGAAGTGTTT
    GCATCATGGA AGCAGCAGTG CCTGAACCGA GGCAAGCAAG ACATCAGTGA GCGGCTCATC
    AGTGCCTCAT TGTTTCTCCG TTTTCTTTGC CCAGCCATTA TGTCTCCCAG TCTTTTCAAC
    CTGATGCAGG AATATCCTGA TGACCGCACA TCTCGGACAC TAACTCTTAT TGCCAAGGTC
    ATTCAGAACC TGGCCAACTT TGCCAAGTTT GGTAACAAAG AGGAGTACAT GGCATTCATG
    AATGATTTTT TAGAACATGA ATGGGGTGGA ATGAAGCGGT TCCTTTTGGA GATCTCTAAT
    CCTGACACCA TCTCAAACAC CCCAGGCTTC GATGGTTACA TTGATTTGGG CAGAGAGCTG
    TCAGTTTTGC ATTCCTTACT ATGGGAAGTA GTTTCCCAAC TTGATAAGGC AACTGTGGCA
    AAACTGGGAC CTCTCCCTCG TGTTCTTGCT GACATTACCA AGTCTCTGAC TAATCCTACG
    CCAATACAGC AGCAGCTAAG ACGTTTCGCT GAGCACAGCT CCAGTCCAAA CGTCAGCGGA
    AGCCTCTCCT CTGGGCTGCA GAGAATATGT GAAGACCCCA CTGACAGTGA TTTGCATAAA
    CTAAAGTCTC CAAGCCAGGA TAACACAGAC AGCTATTTCA GAGGAAAGAC GTTATTGTTG
    GTTCAGCAAG CCTCCTCCCA GAGCATGACG TACTCTGAAA AGGATGAAAA GGAAAACAGC
    CTTCCTAACG GTCGGAGCAT CTCCCTCATG GACCTCCAGG ACACTCATGC TGCTCAGGCG
    GAGCATGCAT CTGTCATGCT TGATGTGCCT ATGCGCCTTG CAGGAAGCCA GCTTTCTATA
    ACCCAGGTAG CTAGCATCAA ACAGCTGCGG GAAACCCAGA GCACTCCCCA GAGTGCACCT
    CAAGTGAGAA GACCCCTGCA CCCAGCCTTG AACCAGCCAG GAAGTCTCCA GCCCTTGTCA
    TTCCAGAATC CTGTCTATCA CCTCAACAAC CCAGTTCCAG CAATGCCAAA GGCCTCTGCG
    GATTCCAGTT TGGAGAACCT AAGCACTGCC AGTTCTAGAA GCCAAAGCAA TAGTGAAGAC
    TTCAAACTCA GTGGACCCAG CAATAGCAGC ATGGAAGATT TCACCAAAAG AAGCTCCCAC
    AGTGAAGACT TCTCCAGGCG CCACACTGTA CCAGACAGAC ACATACCTCT TGCTCTACCA
    CGACAAAATA GTACGGGGCA GTCCCAAATC CGAAAACTGG ACCATTCTGG GTTAGGTGCC
    CGAGCCAAAG CACCCCCATC TCTGCCACAC AGTGCTTCCT TGCGTAGCAC AGGGAGCATG
    TCAGTGGCGT CTGCAGCCCT AATGGCTGAA CCTGTGCAGA ACGGGAGCCG GTCCAGGCAG
    CAGTCCTCTT CCTCCAGAGA GAGCCCTGTT CCCAAAGTGA GAGCAATCCA GAGACAACAG
    ACACAACAGG TTCAGTCACC TGTGGACTCC GCTACAATGT CCCCAGTAGA AAGGACAGCA
    GCCTGGGTTC TGAACAATGG ACAGTATGAA GAGGATGTGG AAGAAACTGA ACAAAATCAA
    GATGAAGCCA AGCATGCTGA AAAGTATGAA CAAGAAATTA CCAAGCTGAA GGAGCGCCTA
    CGAGTGTCCA GCCGGCGGTT GGAGGAGTAT GAGCGCCGGC TGCTGGTGCA GGAGCAGCAG
    ATGCAGAAGC TGCTGCTGGA GTACAAGGCC CGGCTGGAGG ATAGTGAGGA GCGGCTGCGC
    AGACAGCAGG AGGAGAAGGA CAGCCAGATG AAAAGCATCA TCAGCAGGCT AATGGCTGTG
    GAAGAAGAAT TAAAGAAAGA TCATGCTGAG ATGCAAGCAG TTATTGATGC AAAGCAGAAA
    ATAATTGATG CACAGGAAAA GCGGATCGTG TCCCTGGATT CAGCCAACAC GAGACTGATG
    AGTGCGCTGA CACAAGTGAA GGAGCGGTAC AGCATGCAGG TCCGCAATGG CATCTCTCCC
    ACCAACCCCA CCAAGCTTTC CATCACGGAG AATGGTGAAT TCAAAAATAG CAGCTGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu28684
    Clone ID Related Accession (Same CDS sequence) XM_006496753.4 , XM_006496753.3
    Accession Version XM_006496753.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3678bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product ras GTPase-activating protein nGAP isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006496753.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGTACAGA GACAGAATTC AGAGTGGAGG GAGGATCACA TTTTCTTTCA TCATTACAGC 
    CTAATCCACA ATTTTAGTAC TGAACCCCGC CTCCTCCAAG AATCTAGTCT CTTTATCCAT
    TTCTTCCCAC TTCATCATTC AGTAATTGTA TTCTGTATGG AAAAGTTTTC TGGCTGGAGA
    ATGAGTCTCC TTTCCCCTGG TGATGTGTCG GAGTGGTTGG ATAGTAGCAC CGAAGAGGAG
    AAGAGCCTTC TGTCATTTCT GGAGGTGCCA GCAGAAAGGT CCCCCCGCAG ACGGAGCATC
    TCAGGGACCA GCACATCAGA GAAACCCAAC TCCATGGACA CTGCGAATAC CTCTCCTTTT
    AAAGTACCAG GATTCTTCAG CAAGCGCCTG AAAGGTTCCA TCAAGAGGAC CAAAAGCCAG
    TCGAAGCTTG ACAGAAACAC AAGCTTTCGG CTTCCATCCC TTCGCAATGC AGATGACAGG
    TCTCGAGGTC TGCCTAAACT GAAAGAGTCG CGTTCCCATG AGTCCCTGCT GAGCCCATGC
    AGTGCAGTGG AGTGTCTGGA TCTAGGTAGA GGAGAGCCAG TCTCAGTGAA GCCACTGCAC
    AGTAGCATCC TCGGACAGGA CTTCTGCTTC GAGGTCACCT ACTTAAGTGG AAGTAAATGC
    TTTAGCTGTA GCTCTGCCTC CGAGAGAGAT AAGTGGATGG AAAACCTTCG AAGGACCGTT
    CAGCCAAATA AGGACAATTG TAGACGAGCT GAAAATGTTC TCCGTTTATG GATCATTGAA
    GCCAAGGACC TTGCCCCTAA AAAAAAATAT TTCTGCGAAC TGTGCCTTGA TGATACCCTC
    TTTGCTCGTA CAACCAGCAA GACCAAAGCA GACAATATTT TTTGGGGTGA ACATTTTGAA
    TTCTATAGCC TTCCACCTCT TCATAGCATC ACGGTTCATA TTTATAAGGA TGTGGAAAAA
    AAGAAAAAAA AGGACAAGAA TAATTATGTA GGGCTAGTCA ATATCCCCAC TGCCAGTGTG
    ACTGGTCGCC AGTTTGTAGA AAAGTGGTAC CCAGTGAGTA CACCCACACC CAACAAAGGA
    AAGACAGGAG GACCTTCTAT TCGGATTAAG TCACGTTTCC AAACTATCAC CATTCTGCCT
    ATGGAGCAAT ACAAAGAGTT TGCAGAATTC ATCACCAGCA ACTACACCAT GCTGTGTTCT
    GTTCTTGAGC CAGTAATTAG TGTGAGGAAT AAAGAGGAGT TGGCTTGTGC CTTAGTACAC
    ATTCTTCAAA GTACTGGCAG AGCCAAGGAT TTTCTGACTG ACTTGGTGAT GTCAGAGGTA
    GATCGTTGTG GAGAACATGA TGTCTTGATC TTCAGAGAAA ACACCATTGC CACTAAATCC
    ATTGAGGAAT ACCTCAAGTT GGTGGGACAA CAGTACCTTC ATGATGCACT CGGGGAGTTC
    ATCAAAGCCT TGTATGAATC AGATGAGAAC TGTGAAGTGG ATCCCAGCAA GTGTTCATCT
    AGTGAACTGA TGGACCATCA GAGCAACCTG AAAATGTGCT GTGAGTTGGC TTTCTGCAAG
    ATCATCAACT CATACTGTGT CTTCCCGCGT GAGTTGAAAG AAGTGTTTGC ATCATGGAAG
    CAGCAGTGCC TGAACCGAGG CAAGCAAGAC ATCAGTGAGC GGCTCATCAG TGCCTCATTG
    TTTCTCCGTT TTCTTTGCCC AGCCATTATG TCTCCCAGTC TTTTCAACCT GATGCAGGAA
    TATCCTGATG ACCGCACATC TCGGACACTA ACTCTTATTG CCAAGGTCAT TCAGAACCTG
    GCCAACTTTG CCAAGTTTGG TAACAAAGAG GAGTACATGG CATTCATGAA TGATTTTTTA
    GAACATGAAT GGGGTGGAAT GAAGCGGTTC CTTTTGGAGA TCTCTAATCC TGACACCATC
    TCAAACACCC CAGGCTTCGA TGGTTACATT GATTTGGGCA GAGAGCTGTC AGTTTTGCAT
    TCCTTACTAT GGGAAGTAGT TTCCCAACTT GATAAGGGTG AAAATTCCTT TCTACAGGCA
    ACTGTGGCAA AACTGGGACC TCTCCCTCGT GTTCTTGCTG ACATTACCAA GTCTCTGACT
    AATCCTACGC CAATACAGCA GCAGCTAAGA CGTTTCGCTG AGCACAGCTC CAGTCCAAAC
    GTCAGCGGAA GCCTCTCCTC TGGGCTGCAG AGAATATGTG AAGACCCCAC TGACAGTGAT
    TTGCATAAAC TAAAGTCTCC AAGCCAGGAT AACACAGACA GCTATTTCAG AGGAAAGACG
    TTATTGTTGG TTCAGCAAGC CTCCTCCCAG AGCATGACGT ACTCTGAAAA GGATGAAAAG
    GAAAACAGCC TTCCTAACGG TCGGAGCATC TCCCTCATGG ACCTCCAGGA CACTCATGCT
    GCTCAGGCGG AGCATGCATC TGTCATGCTT GATGTGCCTA TGCGCCTTGC AGGAAGCCAG
    CTTTCTATAA CCCAGGTAGC TAGCATCAAA CAGCTGCGGG AAACCCAGAG CACTCCCCAG
    AGTGCACCTC AAGTGAGAAG ACCCCTGCAC CCAGCCTTGA ACCAGCCAGG AAGTCTCCAG
    CCCTTGTCAT TCCAGAATCC TGTCTATCAC CTCAACAACC CAGTTCCAGC AATGCCAAAG
    GCCTCTGCGG ATTCCAGTTT GGAGAACCTA AGCACTGCCA GTTCTAGAAG CCAAAGCAAT
    AGTGAAGACT TCAAACTCAG TGGACCCAGC AATAGCAGCA TGGAAGATTT CACCAAAAGA
    AGCTCCCACA GTGAAGACTT CTCCAGGCGC CACACTGTAC CAGACAGACA CATACCTCTT
    GCTCTACCAC GACAAAATAG TACGGGGCAG TCCCAAATCC GAAAACTGGA CCATTCTGGG
    TTAGGTGCCC GAGCCAAAGC ACCCCCATCT CTGCCACACA GTGCTTCCTT GCGTAGCACA
    GGGAGCATGT CAGTGGCGTC TGCAGCCCTA ATGGCTGAAC CTGTGCAGAA CGGGAGCCGG
    TCCAGGCAGC AGTCCTCTTC CTCCAGAGAG AGCCCTGTTC CCAAAGTGAG AGCAATCCAG
    AGACAACAGA CACAACAGGT TCAGTCACCT GTGGACTCCG CTACAATGTC CCCAGTAGAA
    AGGACAGCAG CCTGGGTTCT GAACAATGGA CAGTATGAAG AGGATGTGGA AGAAACTGAA
    CAAAATCAAG ATGAAGCCAA GCATGCTGAA AAGTATGAAC AAGAAATTAC CAAGCTGAAG
    GAGCGCCTAC GAGTGTCCAG CCGGCGGTTG GAGGAGTATG AGCGCCGGCT GCTGGTGCAG
    GAGCAGCAGA TGCAGAAGCT GCTGCTGGAG TACAAGGCCC GGCTGGAGGA TAGTGAGGAG
    CGGCTGCGCA GACAGCAGGA GGAGAAGGAC AGCCAGATGA AAAGCATCAT CAGCAGGCTA
    ATGGCTGTGG AAGAAGAATT AAAGAAAGAT CATGCTGAGA TGCAAGCAGT TATTGATGCA
    AAGCAGAAAA TAATTGATGC ACAGGTCATA AATGGAAATG AGATTATTCA AACAGGAAAA
    GCGGATCGTG TCCCTGGATT CAGCCAACAC GAGACTGATG AGTGCGCTGA CACAAGTGAA
    GGAGCGGTAC AGCATGCAGG TCCGCAATGG CATCTCTCCC ACCAACCCCA CCAAGCTTTC
    CATCACGGAG AATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006496816.1
    CDS2433..6110
    Translation

    Target ORF information:

    RefSeq Version XM_006496753.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus RAS protein activator like 2 (Rasal2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006496753.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGTACAGA GACAGAATTC AGAGTGGAGG GAGGATCACA TTTTCTTTCA TCATTACAGC 
    CTAATCCACA ATTTTAGTAC TGAACCCCGC CTCCTCCAAG AATCTAGTCT CTTTATCCAT
    TTCTTCCCAC TTCATCATTC AGTAATTGTA TTCTGTATGG AAAAGTTTTC TGGCTGGAGA
    ATGAGTCTCC TTTCCCCTGG TGATGTGTCG GAGTGGTTGG ATAGTAGCAC CGAAGAGGAG
    AAGAGCCTTC TGTCATTTCT GGAGGTGCCA GCAGAAAGGT CCCCCCGCAG ACGGAGCATC
    TCAGGGACCA GCACATCAGA GAAACCCAAC TCCATGGACA CTGCGAATAC CTCTCCTTTT
    AAAGTACCAG GATTCTTCAG CAAGCGCCTG AAAGGTTCCA TCAAGAGGAC CAAAAGCCAG
    TCGAAGCTTG ACAGAAACAC AAGCTTTCGG CTTCCATCCC TTCGCAATGC AGATGACAGG
    TCTCGAGGTC TGCCTAAACT GAAAGAGTCG CGTTCCCATG AGTCCCTGCT GAGCCCATGC
    AGTGCAGTGG AGTGTCTGGA TCTAGGTAGA GGAGAGCCAG TCTCAGTGAA GCCACTGCAC
    AGTAGCATCC TCGGACAGGA CTTCTGCTTC GAGGTCACCT ACTTAAGTGG AAGTAAATGC
    TTTAGCTGTA GCTCTGCCTC CGAGAGAGAT AAGTGGATGG AAAACCTTCG AAGGACCGTT
    CAGCCAAATA AGGACAATTG TAGACGAGCT GAAAATGTTC TCCGTTTATG GATCATTGAA
    GCCAAGGACC TTGCCCCTAA AAAAAAATAT TTCTGCGAAC TGTGCCTTGA TGATACCCTC
    TTTGCTCGTA CAACCAGCAA GACCAAAGCA GACAATATTT TTTGGGGTGA ACATTTTGAA
    TTCTATAGCC TTCCACCTCT TCATAGCATC ACGGTTCATA TTTATAAGGA TGTGGAAAAA
    AAGAAAAAAA AGGACAAGAA TAATTATGTA GGGCTAGTCA ATATCCCCAC TGCCAGTGTG
    ACTGGTCGCC AGTTTGTAGA AAAGTGGTAC CCAGTGAGTA CACCCACACC CAACAAAGGA
    AAGACAGGAG GACCTTCTAT TCGGATTAAG TCACGTTTCC AAACTATCAC CATTCTGCCT
    ATGGAGCAAT ACAAAGAGTT TGCAGAATTC ATCACCAGCA ACTACACCAT GCTGTGTTCT
    GTTCTTGAGC CAGTAATTAG TGTGAGGAAT AAAGAGGAGT TGGCTTGTGC CTTAGTACAC
    ATTCTTCAAA GTACTGGCAG AGCCAAGGAT TTTCTGACTG ACTTGGTGAT GTCAGAGGTA
    GATCGTTGTG GAGAACATGA TGTCTTGATC TTCAGAGAAA ACACCATTGC CACTAAATCC
    ATTGAGGAAT ACCTCAAGTT GGTGGGACAA CAGTACCTTC ATGATGCACT CGGGGAGTTC
    ATCAAAGCCT TGTATGAATC AGATGAGAAC TGTGAAGTGG ATCCCAGCAA GTGTTCATCT
    AGTGAACTGA TGGACCATCA GAGCAACCTG AAAATGTGCT GTGAGTTGGC TTTCTGCAAG
    ATCATCAACT CATACTGTGT CTTCCCGCGT GAGTTGAAAG AAGTGTTTGC ATCATGGAAG
    CAGCAGTGCC TGAACCGAGG CAAGCAAGAC ATCAGTGAGC GGCTCATCAG TGCCTCATTG
    TTTCTCCGTT TTCTTTGCCC AGCCATTATG TCTCCCAGTC TTTTCAACCT GATGCAGGAA
    TATCCTGATG ACCGCACATC TCGGACACTA ACTCTTATTG CCAAGGTCAT TCAGAACCTG
    GCCAACTTTG CCAAGTTTGG TAACAAAGAG GAGTACATGG CATTCATGAA TGATTTTTTA
    GAACATGAAT GGGGTGGAAT GAAGCGGTTC CTTTTGGAGA TCTCTAATCC TGACACCATC
    TCAAACACCC CAGGCTTCGA TGGTTACATT GATTTGGGCA GAGAGCTGTC AGTTTTGCAT
    TCCTTACTAT GGGAAGTAGT TTCCCAACTT GATAAGGGTG AAAATTCCTT TCTACAGGCA
    ACTGTGGCAA AACTGGGACC TCTCCCTCGT GTTCTTGCTG ACATTACCAA GTCTCTGACT
    AATCCTACGC CAATACAGCA GCAGCTAAGA CGTTTCGCTG AGCACAGCTC CAGTCCAAAC
    GTCAGCGGAA GCCTCTCCTC TGGGCTGCAG AGAATATGTG AAGACCCCAC TGACAGTGAT
    TTGCATAAAC TAAAGTCTCC AAGCCAGGAT AACACAGACA GCTATTTCAG AGGAAAGACG
    TTATTGTTGG TTCAGCAAGC CTCCTCCCAG AGCATGACGT ACTCTGAAAA GGATGAAAAG
    GAAAACAGCC TTCCTAACGG TCGGAGCATC TCCCTCATGG ACCTCCAGGA CACTCATGCT
    GCTCAGGCGG AGCATGCATC TGTCATGCTT GATGTGCCTA TGCGCCTTGC AGGAAGCCAG
    CTTTCTATAA CCCAGGTAGC TAGCATCAAA CAGCTGCGGG AAACCCAGAG CACTCCCCAG
    AGTGCACCTC AAGTGAGAAG ACCCCTGCAC CCAGCCTTGA ACCAGCCAGG AAGTCTCCAG
    CCCTTGTCAT TCCAGAATCC TGTCTATCAC CTCAACAACC CAGTTCCAGC AATGCCAAAG
    GCCTCTGCGG ATTCCAGTTT GGAGAACCTA AGCACTGCCA GTTCTAGAAG CCAAAGCAAT
    AGTGAAGACT TCAAACTCAG TGGACCCAGC AATAGCAGCA TGGAAGATTT CACCAAAAGA
    AGCTCCCACA GTGAAGACTT CTCCAGGCGC CACACTGTAC CAGACAGACA CATACCTCTT
    GCTCTACCAC GACAAAATAG TACGGGGCAG TCCCAAATCC GAAAACTGGA CCATTCTGGG
    TTAGGTGCCC GAGCCAAAGC ACCCCCATCT CTGCCACACA GTGCTTCCTT GCGTAGCACA
    GGGAGCATGT CAGTGGCGTC TGCAGCCCTA ATGGCTGAAC CTGTGCAGAA CGGGAGCCGG
    TCCAGGCAGC AGTCCTCTTC CTCCAGAGAG AGCCCTGTTC CCAAAGTGAG AGCAATCCAG
    AGACAACAGA CACAACAGGT TCAGTCACCT GTGGACTCCG CTACAATGTC CCCAGTAGAA
    AGGACAGCAG CCTGGGTTCT GAACAATGGA CAGTATGAAG AGGATGTGGA AGAAACTGAA
    CAAAATCAAG ATGAAGCCAA GCATGCTGAA AAGTATGAAC AAGAAATTAC CAAGCTGAAG
    GAGCGCCTAC GAGTGTCCAG CCGGCGGTTG GAGGAGTATG AGCGCCGGCT GCTGGTGCAG
    GAGCAGCAGA TGCAGAAGCT GCTGCTGGAG TACAAGGCCC GGCTGGAGGA TAGTGAGGAG
    CGGCTGCGCA GACAGCAGGA GGAGAAGGAC AGCCAGATGA AAAGCATCAT CAGCAGGCTA
    ATGGCTGTGG AAGAAGAATT AAAGAAAGAT CATGCTGAGA TGCAAGCAGT TATTGATGCA
    AAGCAGAAAA TAATTGATGC ACAGGTCATA AATGGAAATG AGATTATTCA AACAGGAAAA
    GCGGATCGTG TCCCTGGATT CAGCCAACAC GAGACTGATG AGTGCGCTGA CACAAGTGAA
    GGAGCGGTAC AGCATGCAGG TCCGCAATGG CATCTCTCCC ACCAACCCCA CCAAGCTTTC
    CATCACGGAG AATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu28686
    Clone ID Related Accession (Same CDS sequence) XM_006496755.3 , XM_006496755.2
    Accession Version XM_006496755.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3429bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product ras GTPase-activating protein nGAP isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006496755.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCAGACCC CAGAGGTGCC AGCAGAAAGG TCCCCCCGCA GACGGAGCAT CTCAGGGACC 
    AGCACATCAG AGAAACCCAA CTCCATGGAC ACTGCGAATA CCTCTCCTTT TAAAGTACCA
    GGATTCTTCA GCAAGCGCCT GAAAGGTTCC ATCAAGAGGA CCAAAAGCCA GTCGAAGCTT
    GACAGAAACA CAAGCTTTCG GCTTCCATCC CTTCGCAATG CAGATGACAG GTCTCGAGGT
    CTGCCTAAAC TGAAAGAGTC GCGTTCCCAT GAGTCCCTGC TGAGCCCATG CAGTGCAGTG
    GAGTGTCTGG ATCTAGGTAG AGGAGAGCCA GTCTCAGTGA AGCCACTGCA CAGTAGCATC
    CTCGGACAGG ACTTCTGCTT CGAGGTCACC TACTTAAGTG GAAGTAAATG CTTTAGCTGT
    AGCTCTGCCT CCGAGAGAGA TAAGTGGATG GAAAACCTTC GAAGGACCGT TCAGCCAAAT
    AAGGACAATT GTAGACGAGC TGAAAATGTT CTCCGTTTAT GGATCATTGA AGCCAAGGAC
    CTTGCCCCTA AAAAAAAATA TTTCTGCGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT
    ACAACCAGCA AGACCAAAGC AGACAATATT TTTTGGGGTG AACATTTTGA ATTCTATAGC
    CTTCCACCTC TTCATAGCAT CACGGTTCAT ATTTATAAGG ATGTGGAAAA AAAGAAAAAA
    AAGGACAAGA ATAATTATGT AGGGCTAGTC AATATCCCCA CTGCCAGTGT GACTGGTCGC
    CAGTTTGTAG AAAAGTGGTA CCCAGTGAGT ACACCCACAC CCAACAAAGG AAAGACAGGA
    GGACCTTCTA TTCGGATTAA GTCACGTTTC CAAACTATCA CCATTCTGCC TATGGAGCAA
    TACAAAGAGT TTGCAGAATT CATCACCAGC AACTACACCA TGCTGTGTTC TGTTCTTGAG
    CCAGTAATTA GTGTGAGGAA TAAAGAGGAG TTGGCTTGTG CCTTAGTACA CATTCTTCAA
    AGTACTGGCA GAGCCAAGGA TTTTCTGACT GACTTGGTGA TGTCAGAGGT AGATCGTTGT
    GGAGAACATG ATGTCTTGAT CTTCAGAGAA AACACCATTG CCACTAAATC CATTGAGGAA
    TACCTCAAGT TGGTGGGACA ACAGTACCTT CATGATGCAC TCGGGGAGTT CATCAAAGCC
    TTGTATGAAT CAGATGAGAA CTGTGAAGTG GATCCCAGCA AGTGTTCATC TAGTGAACTG
    ATGGACCATC AGAGCAACCT GAAAATGTGC TGTGAGTTGG CTTTCTGCAA GATCATCAAC
    TCATACTGTG TCTTCCCGCG TGAGTTGAAA GAAGTGTTTG CATCATGGAA GCAGCAGTGC
    CTGAACCGAG GCAAGCAAGA CATCAGTGAG CGGCTCATCA GTGCCTCATT GTTTCTCCGT
    TTTCTTTGCC CAGCCATTAT GTCTCCCAGT CTTTTCAACC TGATGCAGGA ATATCCTGAT
    GACCGCACAT CTCGGACACT AACTCTTATT GCCAAGGTCA TTCAGAACCT GGCCAACTTT
    GCCAAGTTTG GTAACAAAGA GGAGTACATG GCATTCATGA ATGATTTTTT AGAACATGAA
    TGGGGTGGAA TGAAGCGGTT CCTTTTGGAG ATCTCTAATC CTGACACCAT CTCAAACACC
    CCAGGCTTCG ATGGTTACAT TGATTTGGGC AGAGAGCTGT CAGTTTTGCA TTCCTTACTA
    TGGGAAGTAG TTTCCCAACT TGATAAGGGT GAAAATTCCT TTCTACAGGC AACTGTGGCA
    AAACTGGGAC CTCTCCCTCG TGTTCTTGCT GACATTACCA AGTCTCTGAC TAATCCTACG
    CCAATACAGC AGCAGCTAAG ACGTTTCGCT GAGCACAGCT CCAGTCCAAA CGTCAGCGGA
    AGCCTCTCCT CTGGGCTGCA GAGAATATGT GAAGACCCCA CTGACAGTGA TTTGCATAAA
    CTAAAGTCTC CAAGCCAGGA TAACACAGAC AGCTATTTCA GAGGAAAGAC GTTATTGTTG
    GTTCAGCAAG CCTCCTCCCA GAGCATGACG TACTCTGAAA AGGATGAAAA GGAAAACAGC
    CTTCCTAACG GTCGGAGCAT CTCCCTCATG GACCTCCAGG ACACTCATGC TGCTCAGGCG
    GAGCATGCAT CTGTCATGCT TGATGTGCCT ATGCGCCTTG CAGGAAGCCA GCTTTCTATA
    ACCCAGGTAG CTAGCATCAA ACAGCTGCGG GAAACCCAGA GCACTCCCCA GAGTGCACCT
    CAAGTGAGAA GACCCCTGCA CCCAGCCTTG AACCAGCCAG GAAGTCTCCA GCCCTTGTCA
    TTCCAGAATC CTGTCTATCA CCTCAACAAC CCAGTTCCAG CAATGCCAAA GGCCTCTGCG
    GATTCCAGTT TGGAGAACCT AAGCACTGCC AGTTCTAGAA GCCAAAGCAA TAGTGAAGAC
    TTCAAACTCA GTGGACCCAG CAATAGCAGC ATGGAAGATT TCACCAAAAG AAGCTCCCAC
    AGTGAAGACT TCTCCAGGCG CCACACTGTA CCAGACAGAC ACATACCTCT TGCTCTACCA
    CGACAAAATA GTACGGGGCA GTCCCAAATC CGAAAACTGG ACCATTCTGG GTTAGGTGCC
    CGAGCCAAAG CACCCCCATC TCTGCCACAC AGTGCTTCCT TGCGTAGCAC AGGGAGCATG
    TCAGTGGCGT CTGCAGCCCT AATGGCTGAA CCTGTGCAGA ACGGGAGCCG GTCCAGGCAG
    CAGTCCTCTT CCTCCAGAGA GAGCCCTGTT CCCAAAGTGA GAGCAATCCA GAGACAACAG
    ACACAACAGG TTCAGTCACC TGTGGACTCC GCTACAATGT CCCCAGTAGA AAGGACAGCA
    GCCTGGGTTC TGAACAATGG ACAGTATGAA GAGGATGTGG AAGAAACTGA ACAAAATCAA
    GATGAAGCCA AGCATGCTGA AAAGTATGAA CAAGAAATTA CCAAGCTGAA GGAGCGCCTA
    CGAGTGTCCA GCCGGCGGTT GGAGGAGTAT GAGCGCCGGC TGCTGGTGCA GGAGCAGCAG
    ATGCAGAAGC TGCTGCTGGA GTACAAGGCC CGGCTGGAGG ATAGTGAGGA GCGGCTGCGC
    AGACAGCAGG AGGAGAAGGA CAGCCAGATG AAAAGCATCA TCAGCAGGCT AATGGCTGTG
    GAAGAAGAAT TAAAGAAAGA TCATGCTGAG ATGCAAGCAG TTATTGATGC AAAGCAGAAA
    ATAATTGATG CACAGGTCAT AAATGGAAAT GAGATTATTC AAACAGGAAA AGCGGATCGT
    GTCCCTGGAT TCAGCCAACA CGAGACTGAT GAGTGCGCTG ACACAAGTGA AGGAGCGGTA
    CAGCATGCAG GTCCGCAATG GCATCTCTCC CACCAACCCC ACCAAGCTTT CCATCACGGA
    GAATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006496818.1
    CDS182..3610
    Translation

    Target ORF information:

    RefSeq Version XM_006496755.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus RAS protein activator like 2 (Rasal2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006496755.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCAGACCC CAGAGGTGCC AGCAGAAAGG TCCCCCCGCA GACGGAGCAT CTCAGGGACC 
    AGCACATCAG AGAAACCCAA CTCCATGGAC ACTGCGAATA CCTCTCCTTT TAAAGTACCA
    GGATTCTTCA GCAAGCGCCT GAAAGGTTCC ATCAAGAGGA CCAAAAGCCA GTCGAAGCTT
    GACAGAAACA CAAGCTTTCG GCTTCCATCC CTTCGCAATG CAGATGACAG GTCTCGAGGT
    CTGCCTAAAC TGAAAGAGTC GCGTTCCCAT GAGTCCCTGC TGAGCCCATG CAGTGCAGTG
    GAGTGTCTGG ATCTAGGTAG AGGAGAGCCA GTCTCAGTGA AGCCACTGCA CAGTAGCATC
    CTCGGACAGG ACTTCTGCTT CGAGGTCACC TACTTAAGTG GAAGTAAATG CTTTAGCTGT
    AGCTCTGCCT CCGAGAGAGA TAAGTGGATG GAAAACCTTC GAAGGACCGT TCAGCCAAAT
    AAGGACAATT GTAGACGAGC TGAAAATGTT CTCCGTTTAT GGATCATTGA AGCCAAGGAC
    CTTGCCCCTA AAAAAAAATA TTTCTGCGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT
    ACAACCAGCA AGACCAAAGC AGACAATATT TTTTGGGGTG AACATTTTGA ATTCTATAGC
    CTTCCACCTC TTCATAGCAT CACGGTTCAT ATTTATAAGG ATGTGGAAAA AAAGAAAAAA
    AAGGACAAGA ATAATTATGT AGGGCTAGTC AATATCCCCA CTGCCAGTGT GACTGGTCGC
    CAGTTTGTAG AAAAGTGGTA CCCAGTGAGT ACACCCACAC CCAACAAAGG AAAGACAGGA
    GGACCTTCTA TTCGGATTAA GTCACGTTTC CAAACTATCA CCATTCTGCC TATGGAGCAA
    TACAAAGAGT TTGCAGAATT CATCACCAGC AACTACACCA TGCTGTGTTC TGTTCTTGAG
    CCAGTAATTA GTGTGAGGAA TAAAGAGGAG TTGGCTTGTG CCTTAGTACA CATTCTTCAA
    AGTACTGGCA GAGCCAAGGA TTTTCTGACT GACTTGGTGA TGTCAGAGGT AGATCGTTGT
    GGAGAACATG ATGTCTTGAT CTTCAGAGAA AACACCATTG CCACTAAATC CATTGAGGAA
    TACCTCAAGT TGGTGGGACA ACAGTACCTT CATGATGCAC TCGGGGAGTT CATCAAAGCC
    TTGTATGAAT CAGATGAGAA CTGTGAAGTG GATCCCAGCA AGTGTTCATC TAGTGAACTG
    ATGGACCATC AGAGCAACCT GAAAATGTGC TGTGAGTTGG CTTTCTGCAA GATCATCAAC
    TCATACTGTG TCTTCCCGCG TGAGTTGAAA GAAGTGTTTG CATCATGGAA GCAGCAGTGC
    CTGAACCGAG GCAAGCAAGA CATCAGTGAG CGGCTCATCA GTGCCTCATT GTTTCTCCGT
    TTTCTTTGCC CAGCCATTAT GTCTCCCAGT CTTTTCAACC TGATGCAGGA ATATCCTGAT
    GACCGCACAT CTCGGACACT AACTCTTATT GCCAAGGTCA TTCAGAACCT GGCCAACTTT
    GCCAAGTTTG GTAACAAAGA GGAGTACATG GCATTCATGA ATGATTTTTT AGAACATGAA
    TGGGGTGGAA TGAAGCGGTT CCTTTTGGAG ATCTCTAATC CTGACACCAT CTCAAACACC
    CCAGGCTTCG ATGGTTACAT TGATTTGGGC AGAGAGCTGT CAGTTTTGCA TTCCTTACTA
    TGGGAAGTAG TTTCCCAACT TGATAAGGGT GAAAATTCCT TTCTACAGGC AACTGTGGCA
    AAACTGGGAC CTCTCCCTCG TGTTCTTGCT GACATTACCA AGTCTCTGAC TAATCCTACG
    CCAATACAGC AGCAGCTAAG ACGTTTCGCT GAGCACAGCT CCAGTCCAAA CGTCAGCGGA
    AGCCTCTCCT CTGGGCTGCA GAGAATATGT GAAGACCCCA CTGACAGTGA TTTGCATAAA
    CTAAAGTCTC CAAGCCAGGA TAACACAGAC AGCTATTTCA GAGGAAAGAC GTTATTGTTG
    GTTCAGCAAG CCTCCTCCCA GAGCATGACG TACTCTGAAA AGGATGAAAA GGAAAACAGC
    CTTCCTAACG GTCGGAGCAT CTCCCTCATG GACCTCCAGG ACACTCATGC TGCTCAGGCG
    GAGCATGCAT CTGTCATGCT TGATGTGCCT ATGCGCCTTG CAGGAAGCCA GCTTTCTATA
    ACCCAGGTAG CTAGCATCAA ACAGCTGCGG GAAACCCAGA GCACTCCCCA GAGTGCACCT
    CAAGTGAGAA GACCCCTGCA CCCAGCCTTG AACCAGCCAG GAAGTCTCCA GCCCTTGTCA
    TTCCAGAATC CTGTCTATCA CCTCAACAAC CCAGTTCCAG CAATGCCAAA GGCCTCTGCG
    GATTCCAGTT TGGAGAACCT AAGCACTGCC AGTTCTAGAA GCCAAAGCAA TAGTGAAGAC
    TTCAAACTCA GTGGACCCAG CAATAGCAGC ATGGAAGATT TCACCAAAAG AAGCTCCCAC
    AGTGAAGACT TCTCCAGGCG CCACACTGTA CCAGACAGAC ACATACCTCT TGCTCTACCA
    CGACAAAATA GTACGGGGCA GTCCCAAATC CGAAAACTGG ACCATTCTGG GTTAGGTGCC
    CGAGCCAAAG CACCCCCATC TCTGCCACAC AGTGCTTCCT TGCGTAGCAC AGGGAGCATG
    TCAGTGGCGT CTGCAGCCCT AATGGCTGAA CCTGTGCAGA ACGGGAGCCG GTCCAGGCAG
    CAGTCCTCTT CCTCCAGAGA GAGCCCTGTT CCCAAAGTGA GAGCAATCCA GAGACAACAG
    ACACAACAGG TTCAGTCACC TGTGGACTCC GCTACAATGT CCCCAGTAGA AAGGACAGCA
    GCCTGGGTTC TGAACAATGG ACAGTATGAA GAGGATGTGG AAGAAACTGA ACAAAATCAA
    GATGAAGCCA AGCATGCTGA AAAGTATGAA CAAGAAATTA CCAAGCTGAA GGAGCGCCTA
    CGAGTGTCCA GCCGGCGGTT GGAGGAGTAT GAGCGCCGGC TGCTGGTGCA GGAGCAGCAG
    ATGCAGAAGC TGCTGCTGGA GTACAAGGCC CGGCTGGAGG ATAGTGAGGA GCGGCTGCGC
    AGACAGCAGG AGGAGAAGGA CAGCCAGATG AAAAGCATCA TCAGCAGGCT AATGGCTGTG
    GAAGAAGAAT TAAAGAAAGA TCATGCTGAG ATGCAAGCAG TTATTGATGC AAAGCAGAAA
    ATAATTGATG CACAGGTCAT AAATGGAAAT GAGATTATTC AAACAGGAAA AGCGGATCGT
    GTCCCTGGAT TCAGCCAACA CGAGACTGAT GAGTGCGCTG ACACAAGTGA AGGAGCGGTA
    CAGCATGCAG GTCCGCAATG GCATCTCTCC CACCAACCCC ACCAAGCTTT CCATCACGGA
    GAATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu28687
    Clone ID Related Accession (Same CDS sequence) XM_006496756.3 , XM_006496756.4
    Accession Version XM_006496756.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3345bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product ras GTPase-activating protein nGAP isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006496756.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGACACTG CGAATACCTC TCCTTTTAAA GTACCAGGAT TCTTCAGCAA GCGCCTGAAA 
    GGTTCCATCA AGAGGACCAA AAGCCAGTCG AAGCTTGACA GAAACACAAG CTTTCGGCTT
    CCATCCCTTC GCAATGCAGA TGACAGGTCT CGAGGTCTGC CTAAACTGAA AGAGTCGCGT
    TCCCATGAGT CCCTGCTGAG CCCATGCAGT GCAGTGGAGT GTCTGGATCT AGGTAGAGGA
    GAGCCAGTCT CAGTGAAGCC ACTGCACAGT AGCATCCTCG GACAGGACTT CTGCTTCGAG
    GTCACCTACT TAAGTGGAAG TAAATGCTTT AGCTGTAGCT CTGCCTCCGA GAGAGATAAG
    TGGATGGAAA ACCTTCGAAG GACCGTTCAG CCAAATAAGG ACAATTGTAG ACGAGCTGAA
    AATGTTCTCC GTTTATGGAT CATTGAAGCC AAGGACCTTG CCCCTAAAAA AAAATATTTC
    TGCGAACTGT GCCTTGATGA TACCCTCTTT GCTCGTACAA CCAGCAAGAC CAAAGCAGAC
    AATATTTTTT GGGGTGAACA TTTTGAATTC TATAGCCTTC CACCTCTTCA TAGCATCACG
    GTTCATATTT ATAAGGATGT GGAAAAAAAG AAAAAAAAGG ACAAGAATAA TTATGTAGGG
    CTAGTCAATA TCCCCACTGC CAGTGTGACT GGTCGCCAGT TTGTAGAAAA GTGGTACCCA
    GTGAGTACAC CCACACCCAA CAAAGGAAAG ACAGGAGGAC CTTCTATTCG GATTAAGTCA
    CGTTTCCAAA CTATCACCAT TCTGCCTATG GAGCAATACA AAGAGTTTGC AGAATTCATC
    ACCAGCAACT ACACCATGCT GTGTTCTGTT CTTGAGCCAG TAATTAGTGT GAGGAATAAA
    GAGGAGTTGG CTTGTGCCTT AGTACACATT CTTCAAAGTA CTGGCAGAGC CAAGGATTTT
    CTGACTGACT TGGTGATGTC AGAGGTAGAT CGTTGTGGAG AACATGATGT CTTGATCTTC
    AGAGAAAACA CCATTGCCAC TAAATCCATT GAGGAATACC TCAAGTTGGT GGGACAACAG
    TACCTTCATG ATGCACTCGG GGAGTTCATC AAAGCCTTGT ATGAATCAGA TGAGAACTGT
    GAAGTGGATC CCAGCAAGTG TTCATCTAGT GAACTGATGG ACCATCAGAG CAACCTGAAA
    ATGTGCTGTG AGTTGGCTTT CTGCAAGATC ATCAACTCAT ACTGTGTCTT CCCGCGTGAG
    TTGAAAGAAG TGTTTGCATC ATGGAAGCAG CAGTGCCTGA ACCGAGGCAA GCAAGACATC
    AGTGAGCGGC TCATCAGTGC CTCATTGTTT CTCCGTTTTC TTTGCCCAGC CATTATGTCT
    CCCAGTCTTT TCAACCTGAT GCAGGAATAT CCTGATGACC GCACATCTCG GACACTAACT
    CTTATTGCCA AGGTCATTCA GAACCTGGCC AACTTTGCCA AGTTTGGTAA CAAAGAGGAG
    TACATGGCAT TCATGAATGA TTTTTTAGAA CATGAATGGG GTGGAATGAA GCGGTTCCTT
    TTGGAGATCT CTAATCCTGA CACCATCTCA AACACCCCAG GCTTCGATGG TTACATTGAT
    TTGGGCAGAG AGCTGTCAGT TTTGCATTCC TTACTATGGG AAGTAGTTTC CCAACTTGAT
    AAGGGTGAAA ATTCCTTTCT ACAGGCAACT GTGGCAAAAC TGGGACCTCT CCCTCGTGTT
    CTTGCTGACA TTACCAAGTC TCTGACTAAT CCTACGCCAA TACAGCAGCA GCTAAGACGT
    TTCGCTGAGC ACAGCTCCAG TCCAAACGTC AGCGGAAGCC TCTCCTCTGG GCTGCAGAGA
    ATATGTGAAG ACCCCACTGA CAGTGATTTG CATAAACTAA AGTCTCCAAG CCAGGATAAC
    ACAGACAGCT ATTTCAGAGG AAAGACGTTA TTGTTGGTTC AGCAAGCCTC CTCCCAGAGC
    ATGACGTACT CTGAAAAGGA TGAAAAGGAA AACAGCCTTC CTAACGGTCG GAGCATCTCC
    CTCATGGACC TCCAGGACAC TCATGCTGCT CAGGCGGAGC ATGCATCTGT CATGCTTGAT
    GTGCCTATGC GCCTTGCAGG AAGCCAGCTT TCTATAACCC AGGTAGCTAG CATCAAACAG
    CTGCGGGAAA CCCAGAGCAC TCCCCAGAGT GCACCTCAAG TGAGAAGACC CCTGCACCCA
    GCCTTGAACC AGCCAGGAAG TCTCCAGCCC TTGTCATTCC AGAATCCTGT CTATCACCTC
    AACAACCCAG TTCCAGCAAT GCCAAAGGCC TCTGCGGATT CCAGTTTGGA GAACCTAAGC
    ACTGCCAGTT CTAGAAGCCA AAGCAATAGT GAAGACTTCA AACTCAGTGG ACCCAGCAAT
    AGCAGCATGG AAGATTTCAC CAAAAGAAGC TCCCACAGTG AAGACTTCTC CAGGCGCCAC
    ACTGTACCAG ACAGACACAT ACCTCTTGCT CTACCACGAC AAAATAGTAC GGGGCAGTCC
    CAAATCCGAA AACTGGACCA TTCTGGGTTA GGTGCCCGAG CCAAAGCACC CCCATCTCTG
    CCACACAGTG CTTCCTTGCG TAGCACAGGG AGCATGTCAG TGGCGTCTGC AGCCCTAATG
    GCTGAACCTG TGCAGAACGG GAGCCGGTCC AGGCAGCAGT CCTCTTCCTC CAGAGAGAGC
    CCTGTTCCCA AAGTGAGAGC AATCCAGAGA CAACAGACAC AACAGGTTCA GTCACCTGTG
    GACTCCGCTA CAATGTCCCC AGTAGAAAGG ACAGCAGCCT GGGTTCTGAA CAATGGACAG
    TATGAAGAGG ATGTGGAAGA AACTGAACAA AATCAAGATG AAGCCAAGCA TGCTGAAAAG
    TATGAACAAG AAATTACCAA GCTGAAGGAG CGCCTACGAG TGTCCAGCCG GCGGTTGGAG
    GAGTATGAGC GCCGGCTGCT GGTGCAGGAG CAGCAGATGC AGAAGCTGCT GCTGGAGTAC
    AAGGCCCGGC TGGAGGATAG TGAGGAGCGG CTGCGCAGAC AGCAGGAGGA GAAGGACAGC
    CAGATGAAAA GCATCATCAG CAGGCTAATG GCTGTGGAAG AAGAATTAAA GAAAGATCAT
    GCTGAGATGC AAGCAGTTAT TGATGCAAAG CAGAAAATAA TTGATGCACA GGTCATAAAT
    GGAAATGAGA TTATTCAAAC AGGAAAAGCG GATCGTGTCC CTGGATTCAG CCAACACGAG
    ACTGATGAGT GCGCTGACAC AAGTGAAGGA GCGGTACAGC ATGCAGGTCC GCAATGGCAT
    CTCTCCCACC AACCCCACCA AGCTTTCCAT CACGGAGAAT GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006496819.1
    CDS2769..6113
    Translation

    Target ORF information:

    RefSeq Version XM_006496756.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus RAS protein activator like 2 (Rasal2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006496756.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGACACTG CGAATACCTC TCCTTTTAAA GTACCAGGAT TCTTCAGCAA GCGCCTGAAA 
    GGTTCCATCA AGAGGACCAA AAGCCAGTCG AAGCTTGACA GAAACACAAG CTTTCGGCTT
    CCATCCCTTC GCAATGCAGA TGACAGGTCT CGAGGTCTGC CTAAACTGAA AGAGTCGCGT
    TCCCATGAGT CCCTGCTGAG CCCATGCAGT GCAGTGGAGT GTCTGGATCT AGGTAGAGGA
    GAGCCAGTCT CAGTGAAGCC ACTGCACAGT AGCATCCTCG GACAGGACTT CTGCTTCGAG
    GTCACCTACT TAAGTGGAAG TAAATGCTTT AGCTGTAGCT CTGCCTCCGA GAGAGATAAG
    TGGATGGAAA ACCTTCGAAG GACCGTTCAG CCAAATAAGG ACAATTGTAG ACGAGCTGAA
    AATGTTCTCC GTTTATGGAT CATTGAAGCC AAGGACCTTG CCCCTAAAAA AAAATATTTC
    TGCGAACTGT GCCTTGATGA TACCCTCTTT GCTCGTACAA CCAGCAAGAC CAAAGCAGAC
    AATATTTTTT GGGGTGAACA TTTTGAATTC TATAGCCTTC CACCTCTTCA TAGCATCACG
    GTTCATATTT ATAAGGATGT GGAAAAAAAG AAAAAAAAGG ACAAGAATAA TTATGTAGGG
    CTAGTCAATA TCCCCACTGC CAGTGTGACT GGTCGCCAGT TTGTAGAAAA GTGGTACCCA
    GTGAGTACAC CCACACCCAA CAAAGGAAAG ACAGGAGGAC CTTCTATTCG GATTAAGTCA
    CGTTTCCAAA CTATCACCAT TCTGCCTATG GAGCAATACA AAGAGTTTGC AGAATTCATC
    ACCAGCAACT ACACCATGCT GTGTTCTGTT CTTGAGCCAG TAATTAGTGT GAGGAATAAA
    GAGGAGTTGG CTTGTGCCTT AGTACACATT CTTCAAAGTA CTGGCAGAGC CAAGGATTTT
    CTGACTGACT TGGTGATGTC AGAGGTAGAT CGTTGTGGAG AACATGATGT CTTGATCTTC
    AGAGAAAACA CCATTGCCAC TAAATCCATT GAGGAATACC TCAAGTTGGT GGGACAACAG
    TACCTTCATG ATGCACTCGG GGAGTTCATC AAAGCCTTGT ATGAATCAGA TGAGAACTGT
    GAAGTGGATC CCAGCAAGTG TTCATCTAGT GAACTGATGG ACCATCAGAG CAACCTGAAA
    ATGTGCTGTG AGTTGGCTTT CTGCAAGATC ATCAACTCAT ACTGTGTCTT CCCGCGTGAG
    TTGAAAGAAG TGTTTGCATC ATGGAAGCAG CAGTGCCTGA ACCGAGGCAA GCAAGACATC
    AGTGAGCGGC TCATCAGTGC CTCATTGTTT CTCCGTTTTC TTTGCCCAGC CATTATGTCT
    CCCAGTCTTT TCAACCTGAT GCAGGAATAT CCTGATGACC GCACATCTCG GACACTAACT
    CTTATTGCCA AGGTCATTCA GAACCTGGCC AACTTTGCCA AGTTTGGTAA CAAAGAGGAG
    TACATGGCAT TCATGAATGA TTTTTTAGAA CATGAATGGG GTGGAATGAA GCGGTTCCTT
    TTGGAGATCT CTAATCCTGA CACCATCTCA AACACCCCAG GCTTCGATGG TTACATTGAT
    TTGGGCAGAG AGCTGTCAGT TTTGCATTCC TTACTATGGG AAGTAGTTTC CCAACTTGAT
    AAGGGTGAAA ATTCCTTTCT ACAGGCAACT GTGGCAAAAC TGGGACCTCT CCCTCGTGTT
    CTTGCTGACA TTACCAAGTC TCTGACTAAT CCTACGCCAA TACAGCAGCA GCTAAGACGT
    TTCGCTGAGC ACAGCTCCAG TCCAAACGTC AGCGGAAGCC TCTCCTCTGG GCTGCAGAGA
    ATATGTGAAG ACCCCACTGA CAGTGATTTG CATAAACTAA AGTCTCCAAG CCAGGATAAC
    ACAGACAGCT ATTTCAGAGG AAAGACGTTA TTGTTGGTTC AGCAAGCCTC CTCCCAGAGC
    ATGACGTACT CTGAAAAGGA TGAAAAGGAA AACAGCCTTC CTAACGGTCG GAGCATCTCC
    CTCATGGACC TCCAGGACAC TCATGCTGCT CAGGCGGAGC ATGCATCTGT CATGCTTGAT
    GTGCCTATGC GCCTTGCAGG AAGCCAGCTT TCTATAACCC AGGTAGCTAG CATCAAACAG
    CTGCGGGAAA CCCAGAGCAC TCCCCAGAGT GCACCTCAAG TGAGAAGACC CCTGCACCCA
    GCCTTGAACC AGCCAGGAAG TCTCCAGCCC TTGTCATTCC AGAATCCTGT CTATCACCTC
    AACAACCCAG TTCCAGCAAT GCCAAAGGCC TCTGCGGATT CCAGTTTGGA GAACCTAAGC
    ACTGCCAGTT CTAGAAGCCA AAGCAATAGT GAAGACTTCA AACTCAGTGG ACCCAGCAAT
    AGCAGCATGG AAGATTTCAC CAAAAGAAGC TCCCACAGTG AAGACTTCTC CAGGCGCCAC
    ACTGTACCAG ACAGACACAT ACCTCTTGCT CTACCACGAC AAAATAGTAC GGGGCAGTCC
    CAAATCCGAA AACTGGACCA TTCTGGGTTA GGTGCCCGAG CCAAAGCACC CCCATCTCTG
    CCACACAGTG CTTCCTTGCG TAGCACAGGG AGCATGTCAG TGGCGTCTGC AGCCCTAATG
    GCTGAACCTG TGCAGAACGG GAGCCGGTCC AGGCAGCAGT CCTCTTCCTC CAGAGAGAGC
    CCTGTTCCCA AAGTGAGAGC AATCCAGAGA CAACAGACAC AACAGGTTCA GTCACCTGTG
    GACTCCGCTA CAATGTCCCC AGTAGAAAGG ACAGCAGCCT GGGTTCTGAA CAATGGACAG
    TATGAAGAGG ATGTGGAAGA AACTGAACAA AATCAAGATG AAGCCAAGCA TGCTGAAAAG
    TATGAACAAG AAATTACCAA GCTGAAGGAG CGCCTACGAG TGTCCAGCCG GCGGTTGGAG
    GAGTATGAGC GCCGGCTGCT GGTGCAGGAG CAGCAGATGC AGAAGCTGCT GCTGGAGTAC
    AAGGCCCGGC TGGAGGATAG TGAGGAGCGG CTGCGCAGAC AGCAGGAGGA GAAGGACAGC
    CAGATGAAAA GCATCATCAG CAGGCTAATG GCTGTGGAAG AAGAATTAAA GAAAGATCAT
    GCTGAGATGC AAGCAGTTAT TGATGCAAAG CAGAAAATAA TTGATGCACA GGTCATAAAT
    GGAAATGAGA TTATTCAAAC AGGAAAAGCG GATCGTGTCC CTGGATTCAG CCAACACGAG
    ACTGATGAGT GCGCTGACAC AAGTGAAGGA GCGGTACAGC ATGCAGGTCC GCAATGGCAT
    CTCTCCCACC AACCCCACCA AGCTTTCCAT CACGGAGAAT GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu12878
    Clone ID Related Accession (Same CDS sequence) NM_177644.5
    Accession Version NM_177644.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3861bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-12-22
    Organism Mus musculus(house mouse)
    Product ras GTPase-activating protein nGAP
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK039584.1, BC120902.2, AC119220.11 and CA322995.1. On Apr 18, 2009 this sequence version replaced NM_177644.4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC120902.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164138 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCTGT CTCCGTCGTC CGGAGGAGCC GCGGAGGCGC TGTCGTGGCC GGAGATGTTC 
    ACGGGGCTGG AGTCCGACTC GCCGCTGCCC CCGGAGGATC TGGACGCGGT CGTCCCAGTC
    AGCGGAGCAG TGGCCGGTGG CATGCTGGAT CGGATCCTTC TGGAGTCAGT GTGCCAGCAG
    CAGAGCTGGG TCCGGGTGTT CGATGTAAAA GGCCCACCAA CCCACCGTCT GTCTTGTGGT
    CAGTCACCCT ATACTGAGAC GACATCATGG GAGAGGAAGT ACTGCATCCT CACAGACAGC
    CAGTTGGTGT TGCTCAACAA GGAGAAGGAG ATGCCTGTGG AAGGACAGGA TCAACAGACA
    GATTCCACCA AAGGGCGCTG TCTGAGGAGA ACTGTCAGTG TTCCTTCAGA GGGTCAGTTT
    CCGGAGTACC CACAGGAGGG CACCACTAAA CTAGAGGTGC CAGCAGAAAG GTCCCCCCGC
    AGACGGAGCA TCTCAGGGAC CAGCACATCA GAGAAACCCA ACTCCATGGA CACTGCGAAT
    ACCTCTCCTT TTAAAGTACC AGGATTCTTC AGCAAGCGCC TGAAAGGTTC CATCAAGAGG
    ACCAAAAGCC AGTCGAAGCT TGACAGAAAC ACAAGCTTTC GGCTTCCATC CCTTCGCAAT
    GCAGATGACA GGTCTCGAGG TCTGCCTAAA CTGAAAGAGT CGCGTTCCCA TGAGTCCCTG
    CTGAGCCCAT GCAGTGCAGT GGAGTGTCTG GATCTAGGTA GAGGAGAGCC AGTCTCAGTG
    AAGCCACTGC ACAGTAGCAT CCTCGGACAG GACTTCTGCT TCGAGGTCAC CTACTTAAGT
    GGAAGTAAAT GCTTTAGCTG TAGCTCTGCC TCCGAGAGAG ATAAGTGGAT GGAAAACCTT
    CGAAGGACCG TTCAGCCAAA TAAGGACAAT TGTAGACGAG CTGAAAATGT TCTCCGTTTA
    TGGATCATTG AAGCCAAGGA CCTTGCCCCT AAAAAAAAAT ATTTCTGCGA ACTGTGCCTT
    GATGATACCC TCTTTGCTCG TACAACCAGC AAGACCAAAG CAGACAATAT TTTTTGGGGT
    GAACATTTTG AATTCTATAG CCTTCCACCT CTTCATAGCA TCACGGTTCA TATTTATAAG
    GATGTGGAAA AAAAGAAAAA AAAGGACAAG AATAATTATG TAGGGCTAGT CAATATCCCC
    ACTGCCAGTG TGACTGGTCG CCAGTTTGTA GAAAAGTGGT ACCCAGTGAG TACACCCACA
    CCCAACAAAG GAAAGACAGG AGGACCTTCT ATTCGGATTA AGTCACGTTT CCAAACTATC
    ACCATTCTGC CTATGGAGCA ATACAAAGAG TTTGCAGAAT TCATCACCAG CAACTACACC
    ATGCTGTGTT CTGTTCTTGA GCCAGTAATT AGTGTGAGGA ATAAAGAGGA GTTGGCTTGT
    GCCTTAGTAC ACATTCTTCA AAGTACTGGC AGAGCCAAGG ATTTTCTGAC TGACTTGGTG
    ATGTCAGAGG TAGATCGTTG TGGAGAACAT GATGTCTTGA TCTTCAGAGA AAACACCATT
    GCCACTAAAT CCATTGAGGA ATACCTCAAG TTGGTGGGAC AACAGTACCT TCATGATGCA
    CTCGGGGAGT TCATCAAAGC CTTGTATGAA TCAGATGAGA ACTGTGAAGT GGATCCCAGC
    AAGTGTTCAT CTAGTGAACT GATGGACCAT CAGAGCAACC TGAAAATGTG CTGTGAGTTG
    GCTTTCTGCA AGATCATCAA CTCATACTGT GTCTTCCCGC GTGAGTTGAA AGAAGTGTTT
    GCATCATGGA AGCAGCAGTG CCTGAACCGA GGCAAGCAAG ACATCAGTGA GCGGCTCATC
    AGTGCCTCAT TGTTTCTCCG TTTTCTTTGC CCAGCCATTA TGTCTCCCAG TCTTTTCAAC
    CTGATGCAGG AATATCCTGA TGACCGCACA TCTCGGACAC TAACTCTTAT TGCCAAGGTC
    ATTCAGAACC TGGCCAACTT TGCCAAGTTT GGTAACAAAG AGGAGTACAT GGCATTCATG
    AATGATTTTT TAGAACATGA ATGGGGTGGA ATGAAGCGGT TCCTTTTGGA GATCTCTAAT
    CCTGACACCA TCTCAAACAC CCCAGGCTTC GATGGTTACA TTGATTTGGG CAGAGAGCTG
    TCAGTTTTGC ATTCCTTACT ATGGGAAGTA GTTTCCCAAC TTGATAAGGG TGAAAATTCC
    TTTCTACAGG CAACTGTGGC AAAACTGGGA CCTCTCCCTC GTGTTCTTGC TGACATTACC
    AAGTCTCTGA CTAATCCTAC GCCAATACAG CAGCAGCTAA GACGTTTCGC TGAGCACAGC
    TCCAGTCCAA ACGTCAGCGG AAGCCTCTCC TCTGGGCTGC AGAGAATATG TGAAGACCCC
    ACTGACAGTG ATTTGCATAA ACTAAAGTCT CCAAGCCAGG ATAACACAGA CAGCTATTTC
    AGAGGAAAGA CGTTATTGTT GGTTCAGCAA GCCTCCTCCC AGAGCATGAC GTACTCTGAA
    AAGGATGAAA AGGAAAACAG CCTTCCTAAC GGTCGGAGCA TCTCCCTCAT GGACCTCCAG
    GACACTCATG CTGCTCAGGC GGAGCATGCA TCTGTCATGC TTGATGTGCC TATGCGCCTT
    GCAGGAAGCC AGCTTTCTAT AACCCAGGTA GCTAGCATCA AACAGCTGCG GGAAACCCAG
    AGCACTCCCC AGAGTGCACC TCAAGTGAGA AGACCCCTGC ACCCAGCCTT GAACCAGCCA
    GGAAGTCTCC AGCCCTTGTC ATTCCAGAAT CCTGTCTATC ACCTCAACAA CCCAGTTCCA
    GCAATGCCAA AGGCCTCTGC GGATTCCAGT TTGGAGAACC TAAGCACTGC CAGTTCTAGA
    AGCCAAAGCA ATAGTGAAGA CTTCAAACTC AGTGGACCCA GCAATAGCAG CATGGAAGAT
    TTCACCAAAA GAAGCTCCCA CAGTGAAGAC TTCTCCAGGC GCCACACTGT ACCAGACAGA
    CACATACCTC TTGCTCTACC ACGACAAAAT AGTACGGGGC AGTCCCAAAT CCGAAAACTG
    GACCATTCTG GGTTAGGTGC CCGAGCCAAA GCACCCCCAT CTCTGCCACA CAGTGCTTCC
    TTGCGTAGCA CAGGGAGCAT GTCAGTGGCG TCTGCAGCCC TAATGGCTGA ACCTGTGCAG
    AACGGGAGCC GGTCCAGGCA GCAGTCCTCT TCCTCCAGAG AGAGCCCTGT TCCCAAAGTG
    AGAGCAATCC AGAGACAACA GACACAACAG GTTCAGTCAC CTGTGGACTC CGCTACAATG
    TCCCCAGTAG AAAGGACAGC AGCCTGGGTT CTGAACAATG GACAGTATGA AGAGGATGTG
    GAAGAAACTG AACAAAATCA AGATGAAGCC AAGCATGCTG AAAAGTATGA ACAAGAAATT
    ACCAAGCTGA AGGAGCGCCT ACGAGTGTCC AGCCGGCGGT TGGAGGAGTA TGAGCGCCGG
    CTGCTGGTGC AGGAGCAGCA GATGCAGAAG CTGCTGCTGG AGTACAAGGC CCGGCTGGAG
    GATAGTGAGG AGCGGCTGCG CAGACAGCAG GAGGAGAAGG ACAGCCAGAT GAAAAGCATC
    ATCAGCAGGC TAATGGCTGT GGAAGAAGAA TTAAAGAAAG ATCATGCTGA GATGCAAGCA
    GTTATTGATG CAAAGCAGAA AATAATTGAT GCACAGGAAA AGCGGATCGT GTCCCTGGAT
    TCAGCCAACA CGAGACTGAT GAGTGCGCTG ACACAAGTGA AGGAGCGGTA CAGCATGCAG
    GTCCGCAATG GCATCTCTCC CACCAACCCC ACCAAGCTTT CCATCACGGA GAATGGTGAA
    TTCAAAAATA GCAGCTGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_808312.3
    CDS658..4518
    Translation

    Target ORF information:

    RefSeq Version NM_177644.5
    Organism Mus musculus(house mouse)
    Definition Mus musculus RAS protein activator like 2 (Rasal2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_177644.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCTGT CTCCGTCGTC CGGAGGAGCC GCGGAGGCGC TGTCGTGGCC GGAGATGTTC 
    ACGGGGCTGG AGTCCGACTC GCCGCTGCCC CCGGAGGATC TGGACGCGGT CGTCCCAGTC
    AGCGGAGCAG TGGCCGGTGG CATGCTGGAT CGGATCCTTC TGGAGTCAGT GTGCCAGCAG
    CAGAGCTGGG TCCGGGTGTT CGATGTAAAA GGCCCACCAA CCCACCGTCT GTCTTGTGGT
    CAGTCACCCT ATACTGAGAC GACATCATGG GAGAGGAAGT ACTGCATCCT CACAGACAGC
    CAGTTGGTGT TGCTCAACAA GGAGAAGGAG ATGCCTGTGG AAGGACAGGA TCAACAGACA
    GATTCCACCA AAGGGCGCTG TCTGAGGAGA ACTGTCAGTG TTCCTTCAGA GGGTCAGTTT
    CCGGAGTACC CACAGGAGGG CACCACTAAA CTAGAGGTGC CAGCAGAAAG GTCCCCCCGC
    AGACGGAGCA TCTCAGGGAC CAGCACATCA GAGAAACCCA ACTCCATGGA CACTGCGAAT
    ACCTCTCCTT TTAAAGTACC AGGATTCTTC AGCAAGCGCC TGAAAGGTTC CATCAAGAGG
    ACCAAAAGCC AGTCGAAGCT TGACAGAAAC ACAAGCTTTC GGCTTCCATC CCTTCGCAAT
    GCAGATGACA GGTCTCGAGG TCTGCCTAAA CTGAAAGAGT CGCGTTCCCA TGAGTCCCTG
    CTGAGCCCAT GCAGTGCAGT GGAGTGTCTG GATCTAGGTA GAGGAGAGCC AGTCTCAGTG
    AAGCCACTGC ACAGTAGCAT CCTCGGACAG GACTTCTGCT TCGAGGTCAC CTACTTAAGT
    GGAAGTAAAT GCTTTAGCTG TAGCTCTGCC TCCGAGAGAG ATAAGTGGAT GGAAAACCTT
    CGAAGGACCG TTCAGCCAAA TAAGGACAAT TGTAGACGAG CTGAAAATGT TCTCCGTTTA
    TGGATCATTG AAGCCAAGGA CCTTGCCCCT AAAAAAAAAT ATTTCTGCGA ACTGTGCCTT
    GATGATACCC TCTTTGCTCG TACAACCAGC AAGACCAAAG CAGACAATAT TTTTTGGGGT
    GAACATTTTG AATTCTATAG CCTTCCACCT CTTCATAGCA TCACGGTTCA TATTTATAAG
    GATGTGGAAA AAAAGAAAAA AAAGGACAAG AATAATTATG TAGGGCTAGT CAATATCCCC
    ACTGCCAGTG TGACTGGTCG CCAGTTTGTA GAAAAGTGGT ACCCAGTGAG TACACCCACA
    CCCAACAAAG GAAAGACAGG AGGACCTTCT ATTCGGATTA AGTCACGTTT CCAAACTATC
    ACCATTCTGC CTATGGAGCA ATACAAAGAG TTTGCAGAAT TCATCACCAG CAACTACACC
    ATGCTGTGTT CTGTTCTTGA GCCAGTAATT AGTGTGAGGA ATAAAGAGGA GTTGGCTTGT
    GCCTTAGTAC ACATTCTTCA AAGTACTGGC AGAGCCAAGG ATTTTCTGAC TGACTTGGTG
    ATGTCAGAGG TAGATCGTTG TGGAGAACAT GATGTCTTGA TCTTCAGAGA AAACACCATT
    GCCACTAAAT CCATTGAGGA ATACCTCAAG TTGGTGGGAC AACAGTACCT TCATGATGCA
    CTCGGGGAGT TCATCAAAGC CTTGTATGAA TCAGATGAGA ACTGTGAAGT GGATCCCAGC
    AAGTGTTCAT CTAGTGAACT GATGGACCAT CAGAGCAACC TGAAAATGTG CTGTGAGTTG
    GCTTTCTGCA AGATCATCAA CTCATACTGT GTCTTCCCGC GTGAGTTGAA AGAAGTGTTT
    GCATCATGGA AGCAGCAGTG CCTGAACCGA GGCAAGCAAG ACATCAGTGA GCGGCTCATC
    AGTGCCTCAT TGTTTCTCCG TTTTCTTTGC CCAGCCATTA TGTCTCCCAG TCTTTTCAAC
    CTGATGCAGG AATATCCTGA TGACCGCACA TCTCGGACAC TAACTCTTAT TGCCAAGGTC
    ATTCAGAACC TGGCCAACTT TGCCAAGTTT GGTAACAAAG AGGAGTACAT GGCATTCATG
    AATGATTTTT TAGAACATGA ATGGGGTGGA ATGAAGCGGT TCCTTTTGGA GATCTCTAAT
    CCTGACACCA TCTCAAACAC CCCAGGCTTC GATGGTTACA TTGATTTGGG CAGAGAGCTG
    TCAGTTTTGC ATTCCTTACT ATGGGAAGTA GTTTCCCAAC TTGATAAGGG TGAAAATTCC
    TTTCTACAGG CAACTGTGGC AAAACTGGGA CCTCTCCCTC GTGTTCTTGC TGACATTACC
    AAGTCTCTGA CTAATCCTAC GCCAATACAG CAGCAGCTAA GACGTTTCGC TGAGCACAGC
    TCCAGTCCAA ACGTCAGCGG AAGCCTCTCC TCTGGGCTGC AGAGAATATG TGAAGACCCC
    ACTGACAGTG ATTTGCATAA ACTAAAGTCT CCAAGCCAGG ATAACACAGA CAGCTATTTC
    AGAGGAAAGA CGTTATTGTT GGTTCAGCAA GCCTCCTCCC AGAGCATGAC GTACTCTGAA
    AAGGATGAAA AGGAAAACAG CCTTCCTAAC GGTCGGAGCA TCTCCCTCAT GGACCTCCAG
    GACACTCATG CTGCTCAGGC GGAGCATGCA TCTGTCATGC TTGATGTGCC TATGCGCCTT
    GCAGGAAGCC AGCTTTCTAT AACCCAGGTA GCTAGCATCA AACAGCTGCG GGAAACCCAG
    AGCACTCCCC AGAGTGCACC TCAAGTGAGA AGACCCCTGC ACCCAGCCTT GAACCAGCCA
    GGAAGTCTCC AGCCCTTGTC ATTCCAGAAT CCTGTCTATC ACCTCAACAA CCCAGTTCCA
    GCAATGCCAA AGGCCTCTGC GGATTCCAGT TTGGAGAACC TAAGCACTGC CAGTTCTAGA
    AGCCAAAGCA ATAGTGAAGA CTTCAAACTC AGTGGACCCA GCAATAGCAG CATGGAAGAT
    TTCACCAAAA GAAGCTCCCA CAGTGAAGAC TTCTCCAGGC GCCACACTGT ACCAGACAGA
    CACATACCTC TTGCTCTACC ACGACAAAAT AGTACGGGGC AGTCCCAAAT CCGAAAACTG
    GACCATTCTG GGTTAGGTGC CCGAGCCAAA GCACCCCCAT CTCTGCCACA CAGTGCTTCC
    TTGCGTAGCA CAGGGAGCAT GTCAGTGGCG TCTGCAGCCC TAATGGCTGA ACCTGTGCAG
    AACGGGAGCC GGTCCAGGCA GCAGTCCTCT TCCTCCAGAG AGAGCCCTGT TCCCAAAGTG
    AGAGCAATCC AGAGACAACA GACACAACAG GTTCAGTCAC CTGTGGACTC CGCTACAATG
    TCCCCAGTAG AAAGGACAGC AGCCTGGGTT CTGAACAATG GACAGTATGA AGAGGATGTG
    GAAGAAACTG AACAAAATCA AGATGAAGCC AAGCATGCTG AAAAGTATGA ACAAGAAATT
    ACCAAGCTGA AGGAGCGCCT ACGAGTGTCC AGCCGGCGGT TGGAGGAGTA TGAGCGCCGG
    CTGCTGGTGC AGGAGCAGCA GATGCAGAAG CTGCTGCTGG AGTACAAGGC CCGGCTGGAG
    GATAGTGAGG AGCGGCTGCG CAGACAGCAG GAGGAGAAGG ACAGCCAGAT GAAAAGCATC
    ATCAGCAGGC TAATGGCTGT GGAAGAAGAA TTAAAGAAAG ATCATGCTGA GATGCAAGCA
    GTTATTGATG CAAAGCAGAA AATAATTGAT GCACAGGAAA AGCGGATCGT GTCCCTGGAT
    TCAGCCAACA CGAGACTGAT GAGTGCGCTG ACACAAGTGA AGGAGCGGTA CAGCATGCAG
    GTCCGCAATG GCATCTCTCC CACCAACCCC ACCAAGCTTT CCATCACGGA GAATGGTGAA
    TTCAAAAATA GCAGCTGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu28681
    Clone ID Related Accession (Same CDS sequence) XM_006496750.1 , XM_006496750.2
    Accession Version XM_006496750.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3870bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product ras GTPase-activating protein nGAP isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCTGT CTCCGTCGTC CGGAGGAGCC GCGGAGGCGC TGTCGTGGCC GGAGATGTTC 
    ACGGGGCTGG AGTCCGACTC GCCGCTGCCC CCGGAGGATC TGGACGCGGT CGTCCCAGTC
    AGCGGAGCAG TGGCCGGTGG CATGCTGGAT CGGATCCTTC TGGAGTCAGT GTGCCAGCAG
    CAGAGCTGGG TCCGGGTGTT CGATGTAAAA GGCCCACCAA CCCACCGTCT GTCTTGTGGT
    CAGTCACCCT ATACTGAGAC GACATCATGG GAGAGGAAGT ACTGCATCCT CACAGACAGC
    CAGTTGGTGT TGCTCAACAA GGAGAAGGAG ATGCCTGTGG AAGGACAGGA TCAACAGACA
    GATTCCACCA AAGGGCGCTG TCTGAGGAGA ACTGTCAGTG TTCCTTCAGA GGGTCAGTTT
    CCGGAGTACC CACAGGAGGG CACCACTAAA CTAGAGGTGC CAGCAGAAAG GTCCCCCCGC
    AGACGGAGCA TCTCAGGGAC CAGCACATCA GAGAAACCCA ACTCCATGGA CACTGCGAAT
    ACCTCTCCTT TTAAAGTACC AGGATTCTTC AGCAAGCGCC TGAAAGGTTC CATCAAGAGG
    ACCAAAAGCC AGTCGAAGCT TGACAGAAAC ACAAGCTTTC GGCTTCCATC CCTTCGCAAT
    GCAGATGACA GGTCTCGAGG TCTGCCTAAA CTGAAAGAGT CGCGTTCCCA TGAGTCCCTG
    CTGAGCCCAT GCAGTGCAGT GGAGTGTCTG GATCTAGGTA GAGGAGAGCC AGTCTCAGTG
    AAGCCACTGC ACAGTAGCAT CCTCGGACAG GACTTCTGCT TCGAGGTCAC CTACTTAAGT
    GGAAGTAAAT GCTTTAGCTG TAGCTCTGCC TCCGAGAGAG ATAAGTGGAT GGAAAACCTT
    CGAAGGACCG TTCAGCCAAA TAAGGACAAT TGTAGACGAG CTGAAAATGT TCTCCGTTTA
    TGGATCATTG AAGCCAAGGA CCTTGCCCCT AAAAAAAAAT ATTTCTGCGA ACTGTGCCTT
    GATGATACCC TCTTTGCTCG TACAACCAGC AAGACCAAAG CAGACAATAT TTTTTGGGGT
    GAACATTTTG AATTCTATAG CCTTCCACCT CTTCATAGCA TCACGGTTCA TATTTATAAG
    GATGTGGAAA AAAAGAAAAA AAAGGACAAG AATAATTATG TAGGGCTAGT CAATATCCCC
    ACTGCCAGTG TGACTGGTCG CCAGTTTGTA GAAAAGTGGT ACCCAGTGAG TACACCCACA
    CCCAACAAAG GAAAGACAGG AGGACCTTCT ATTCGGATTA AGTCACGTTT CCAAACTATC
    ACCATTCTGC CTATGGAGCA ATACAAAGAG TTTGCAGAAT TCATCACCAG CAACTACACC
    ATGCTGTGTT CTGTTCTTGA GCCAGTAATT AGTGTGAGGA ATAAAGAGGA GTTGGCTTGT
    GCCTTAGTAC ACATTCTTCA AAGTACTGGC AGAGCCAAGG ATTTTCTGAC TGACTTGGTG
    ATGTCAGAGG TAGATCGTTG TGGAGAACAT GATGTCTTGA TCTTCAGAGA AAACACCATT
    GCCACTAAAT CCATTGAGGA ATACCTCAAG TTGGTGGGAC AACAGTACCT TCATGATGCA
    CTCGGGGAGT TCATCAAAGC CTTGTATGAA TCAGATGAGA ACTGTGAAGT GGATCCCAGC
    AAGTGTTCAT CTAGTGAACT GATGGACCAT CAGAGCAACC TGAAAATGTG CTGTGAGTTG
    GCTTTCTGCA AGATCATCAA CTCATACTGT GTCTTCCCGC GTGAGTTGAA AGAAGTGTTT
    GCATCATGGA AGCAGCAGTG CCTGAACCGA GGCAAGCAAG ACATCAGTGA GCGGCTCATC
    AGTGCCTCAT TGTTTCTCCG TTTTCTTTGC CCAGCCATTA TGTCTCCCAG TCTTTTCAAC
    CTGATGCAGG AATATCCTGA TGACCGCACA TCTCGGACAC TAACTCTTAT TGCCAAGGTC
    ATTCAGAACC TGGCCAACTT TGCCAAGTTT GGTAACAAAG AGGAGTACAT GGCATTCATG
    AATGATTTTT TAGAACATGA ATGGGGTGGA ATGAAGCGGT TCCTTTTGGA GATCTCTAAT
    CCTGACACCA TCTCAAACAC CCCAGGCTTC GATGGTTACA TTGATTTGGG CAGAGAGCTG
    TCAGTTTTGC ATTCCTTACT ATGGGAAGTA GTTTCCCAAC TTGATAAGGG TGAAAATTCC
    TTTCTACAGG CAACTGTGGC AAAACTGGGA CCTCTCCCTC GTGTTCTTGC TGACATTACC
    AAGTCTCTGA CTAATCCTAC GCCAATACAG CAGCAGCTAA GACGTTTCGC TGAGCACAGC
    TCCAGTCCAA ACGTCAGCGG AAGCCTCTCC TCTGGGCTGC AGAGAATATG TGAAGACCCC
    ACTGACAGTG ATTTGCATAA ACTAAAGTCT CCAAGCCAGG ATAACACAGA CAGCTATTTC
    AGAGGAAAGA CGTTATTGTT GGTTCAGCAA GCCTCCTCCC AGAGCATGAC GTACTCTGAA
    AAGGATGAAA AGGAAAACAG CCTTCCTAAC GGTCGGAGCA TCTCCCTCAT GGACCTCCAG
    GACACTCATG CTGCTCAGGC GGAGCATGCA TCTGTCATGC TTGATGTGCC TATGCGCCTT
    GCAGGAAGCC AGCTTTCTAT AACCCAGGTA GCTAGCATCA AACAGCTGCG GGAAACCCAG
    AGCACTCCCC AGAGTGCACC TCAAGTGAGA AGACCCCTGC ACCCAGCCTT GAACCAGCCA
    GGAAGTCTCC AGCCCTTGTC ATTCCAGAAT CCTGTCTATC ACCTCAACAA CCCAGTTCCA
    GCAATGCCAA AGGCCTCTGC GGATTCCAGT TTGGAGAACC TAAGCACTGC CAGTTCTAGA
    AGCCAAAGCA ATAGTGAAGA CTTCAAACTC AGTGGACCCA GCAATAGCAG CATGGAAGAT
    TTCACCAAAA GAAGCTCCCA CAGTGAAGAC TTCTCCAGGC GCCACACTGT ACCAGACAGA
    CACATACCTC TTGCTCTACC ACGACAAAAT AGTACGGGGC AGTCCCAAAT CCGAAAACTG
    GACCATTCTG GGTTAGGTGC CCGAGCCAAA GCACCCCCAT CTCTGCCACA CAGTGCTTCC
    TTGCGTAGCA CAGGGAGCAT GTCAGTGGCG TCTGCAGCCC TAATGGCTGA ACCTGTGCAG
    AACGGGAGCC GGTCCAGGCA GCAGTCCTCT TCCTCCAGAG AGAGCCCTGT TCCCAAAGTG
    AGAGCAATCC AGAGACAACA GACACAACAG GTTCAGTCAC CTGTGGACTC CGCTACAATG
    TCCCCAGTAG AAAGGACAGC AGCCTGGGTT CTGAACAATG GACAGTATGA AGAGGATGTG
    GAAGAAACTG AACAAAATCA AGATGAAGCC AAGCATGCTG AAAAGTATGA ACAAGAAATT
    ACCAAGCTGA AGGAGCGCCT ACGAGTGTCC AGCCGGCGGT TGGAGGAGTA TGAGCGCCGG
    CTGCTGGTGC AGGAGCAGCA GATGCAGAAG CTGCTGCTGG AGTACAAGGC CCGGCTGGAG
    GATAGTGAGG AGCGGCTGCG CAGACAGCAG GAGGAGAAGG ACAGCCAGAT GAAAAGCATC
    ATCAGCAGGC TAATGGCTGT GGAAGAAGAA TTAAAGAAAG ATCATGCTGA GATGCAAGCA
    GTTATTGATG CAAAGCAGAA AATAATTGAT GCACAGGTCA TAAATGGAAA TGAGATTATT
    CAAACAGGAA AAGCGGATCG TGTCCCTGGA TTCAGCCAAC ACGAGACTGA TGAGTGCGCT
    GACACAAGTG AAGGAGCGGT ACAGCATGCA GGTCCGCAAT GGCATCTCTC CCACCAACCC
    CACCAAGCTT TCCATCACGG AGAATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006496813.1
    CDS284..4153
    Translation

    Target ORF information:

    RefSeq Version XM_006496750.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus RAS protein activator like 2 (Rasal2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006496750.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCTGT CTCCGTCGTC CGGAGGAGCC GCGGAGGCGC TGTCGTGGCC GGAGATGTTC 
    ACGGGGCTGG AGTCCGACTC GCCGCTGCCC CCGGAGGATC TGGACGCGGT CGTCCCAGTC
    AGCGGAGCAG TGGCCGGTGG CATGCTGGAT CGGATCCTTC TGGAGTCAGT GTGCCAGCAG
    CAGAGCTGGG TCCGGGTGTT CGATGTAAAA GGCCCACCAA CCCACCGTCT GTCTTGTGGT
    CAGTCACCCT ATACTGAGAC GACATCATGG GAGAGGAAGT ACTGCATCCT CACAGACAGC
    CAGTTGGTGT TGCTCAACAA GGAGAAGGAG ATGCCTGTGG AAGGACAGGA TCAACAGACA
    GATTCCACCA AAGGGCGCTG TCTGAGGAGA ACTGTCAGTG TTCCTTCAGA GGGTCAGTTT
    CCGGAGTACC CACAGGAGGG CACCACTAAA CTAGAGGTGC CAGCAGAAAG GTCCCCCCGC
    AGACGGAGCA TCTCAGGGAC CAGCACATCA GAGAAACCCA ACTCCATGGA CACTGCGAAT
    ACCTCTCCTT TTAAAGTACC AGGATTCTTC AGCAAGCGCC TGAAAGGTTC CATCAAGAGG
    ACCAAAAGCC AGTCGAAGCT TGACAGAAAC ACAAGCTTTC GGCTTCCATC CCTTCGCAAT
    GCAGATGACA GGTCTCGAGG TCTGCCTAAA CTGAAAGAGT CGCGTTCCCA TGAGTCCCTG
    CTGAGCCCAT GCAGTGCAGT GGAGTGTCTG GATCTAGGTA GAGGAGAGCC AGTCTCAGTG
    AAGCCACTGC ACAGTAGCAT CCTCGGACAG GACTTCTGCT TCGAGGTCAC CTACTTAAGT
    GGAAGTAAAT GCTTTAGCTG TAGCTCTGCC TCCGAGAGAG ATAAGTGGAT GGAAAACCTT
    CGAAGGACCG TTCAGCCAAA TAAGGACAAT TGTAGACGAG CTGAAAATGT TCTCCGTTTA
    TGGATCATTG AAGCCAAGGA CCTTGCCCCT AAAAAAAAAT ATTTCTGCGA ACTGTGCCTT
    GATGATACCC TCTTTGCTCG TACAACCAGC AAGACCAAAG CAGACAATAT TTTTTGGGGT
    GAACATTTTG AATTCTATAG CCTTCCACCT CTTCATAGCA TCACGGTTCA TATTTATAAG
    GATGTGGAAA AAAAGAAAAA AAAGGACAAG AATAATTATG TAGGGCTAGT CAATATCCCC
    ACTGCCAGTG TGACTGGTCG CCAGTTTGTA GAAAAGTGGT ACCCAGTGAG TACACCCACA
    CCCAACAAAG GAAAGACAGG AGGACCTTCT ATTCGGATTA AGTCACGTTT CCAAACTATC
    ACCATTCTGC CTATGGAGCA ATACAAAGAG TTTGCAGAAT TCATCACCAG CAACTACACC
    ATGCTGTGTT CTGTTCTTGA GCCAGTAATT AGTGTGAGGA ATAAAGAGGA GTTGGCTTGT
    GCCTTAGTAC ACATTCTTCA AAGTACTGGC AGAGCCAAGG ATTTTCTGAC TGACTTGGTG
    ATGTCAGAGG TAGATCGTTG TGGAGAACAT GATGTCTTGA TCTTCAGAGA AAACACCATT
    GCCACTAAAT CCATTGAGGA ATACCTCAAG TTGGTGGGAC AACAGTACCT TCATGATGCA
    CTCGGGGAGT TCATCAAAGC CTTGTATGAA TCAGATGAGA ACTGTGAAGT GGATCCCAGC
    AAGTGTTCAT CTAGTGAACT GATGGACCAT CAGAGCAACC TGAAAATGTG CTGTGAGTTG
    GCTTTCTGCA AGATCATCAA CTCATACTGT GTCTTCCCGC GTGAGTTGAA AGAAGTGTTT
    GCATCATGGA AGCAGCAGTG CCTGAACCGA GGCAAGCAAG ACATCAGTGA GCGGCTCATC
    AGTGCCTCAT TGTTTCTCCG TTTTCTTTGC CCAGCCATTA TGTCTCCCAG TCTTTTCAAC
    CTGATGCAGG AATATCCTGA TGACCGCACA TCTCGGACAC TAACTCTTAT TGCCAAGGTC
    ATTCAGAACC TGGCCAACTT TGCCAAGTTT GGTAACAAAG AGGAGTACAT GGCATTCATG
    AATGATTTTT TAGAACATGA ATGGGGTGGA ATGAAGCGGT TCCTTTTGGA GATCTCTAAT
    CCTGACACCA TCTCAAACAC CCCAGGCTTC GATGGTTACA TTGATTTGGG CAGAGAGCTG
    TCAGTTTTGC ATTCCTTACT ATGGGAAGTA GTTTCCCAAC TTGATAAGGG TGAAAATTCC
    TTTCTACAGG CAACTGTGGC AAAACTGGGA CCTCTCCCTC GTGTTCTTGC TGACATTACC
    AAGTCTCTGA CTAATCCTAC GCCAATACAG CAGCAGCTAA GACGTTTCGC TGAGCACAGC
    TCCAGTCCAA ACGTCAGCGG AAGCCTCTCC TCTGGGCTGC AGAGAATATG TGAAGACCCC
    ACTGACAGTG ATTTGCATAA ACTAAAGTCT CCAAGCCAGG ATAACACAGA CAGCTATTTC
    AGAGGAAAGA CGTTATTGTT GGTTCAGCAA GCCTCCTCCC AGAGCATGAC GTACTCTGAA
    AAGGATGAAA AGGAAAACAG CCTTCCTAAC GGTCGGAGCA TCTCCCTCAT GGACCTCCAG
    GACACTCATG CTGCTCAGGC GGAGCATGCA TCTGTCATGC TTGATGTGCC TATGCGCCTT
    GCAGGAAGCC AGCTTTCTAT AACCCAGGTA GCTAGCATCA AACAGCTGCG GGAAACCCAG
    AGCACTCCCC AGAGTGCACC TCAAGTGAGA AGACCCCTGC ACCCAGCCTT GAACCAGCCA
    GGAAGTCTCC AGCCCTTGTC ATTCCAGAAT CCTGTCTATC ACCTCAACAA CCCAGTTCCA
    GCAATGCCAA AGGCCTCTGC GGATTCCAGT TTGGAGAACC TAAGCACTGC CAGTTCTAGA
    AGCCAAAGCA ATAGTGAAGA CTTCAAACTC AGTGGACCCA GCAATAGCAG CATGGAAGAT
    TTCACCAAAA GAAGCTCCCA CAGTGAAGAC TTCTCCAGGC GCCACACTGT ACCAGACAGA
    CACATACCTC TTGCTCTACC ACGACAAAAT AGTACGGGGC AGTCCCAAAT CCGAAAACTG
    GACCATTCTG GGTTAGGTGC CCGAGCCAAA GCACCCCCAT CTCTGCCACA CAGTGCTTCC
    TTGCGTAGCA CAGGGAGCAT GTCAGTGGCG TCTGCAGCCC TAATGGCTGA ACCTGTGCAG
    AACGGGAGCC GGTCCAGGCA GCAGTCCTCT TCCTCCAGAG AGAGCCCTGT TCCCAAAGTG
    AGAGCAATCC AGAGACAACA GACACAACAG GTTCAGTCAC CTGTGGACTC CGCTACAATG
    TCCCCAGTAG AAAGGACAGC AGCCTGGGTT CTGAACAATG GACAGTATGA AGAGGATGTG
    GAAGAAACTG AACAAAATCA AGATGAAGCC AAGCATGCTG AAAAGTATGA ACAAGAAATT
    ACCAAGCTGA AGGAGCGCCT ACGAGTGTCC AGCCGGCGGT TGGAGGAGTA TGAGCGCCGG
    CTGCTGGTGC AGGAGCAGCA GATGCAGAAG CTGCTGCTGG AGTACAAGGC CCGGCTGGAG
    GATAGTGAGG AGCGGCTGCG CAGACAGCAG GAGGAGAAGG ACAGCCAGAT GAAAAGCATC
    ATCAGCAGGC TAATGGCTGT GGAAGAAGAA TTAAAGAAAG ATCATGCTGA GATGCAAGCA
    GTTATTGATG CAAAGCAGAA AATAATTGAT GCACAGGTCA TAAATGGAAA TGAGATTATT
    CAAACAGGAA AAGCGGATCG TGTCCCTGGA TTCAGCCAAC ACGAGACTGA TGAGTGCGCT
    GACACAAGTG AAGGAGCGGT ACAGCATGCA GGTCCGCAAT GGCATCTCTC CCACCAACCC
    CACCAAGCTT TCCATCACGG AGAATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu28682
    Clone ID Related Accession (Same CDS sequence) XM_006496751.2 , XM_006496751.1
    Accession Version XM_006496751.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3849bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product ras GTPase-activating protein nGAP isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCTGT CTCCGTCGTC CGGAGGAGCC GCGGAGGCGC TGTCGTGGCC GGAGATGTTC 
    ACGGGGCTGG AGTCCGACTC GCCGCTGCCC CCGGAGGATC TGGACGCGGT CGTCCCAGTC
    AGCGGAGCAG TGGCCGGTGG CATGCTGGAT CGGATCCTTC TGGAGTCAGT GTGCCAGCAG
    CAGAGCTGGG TCCGGGTGTT CGATGTAAAA GGCCCACCAA CCCACCGTCT GTCTTGTGGT
    CAGTCACCCT ATACTGAGAC GACATCATGG GAGAGGAAGT ACTGCATCCT CACAGACAGC
    CAGTTGGTGT TGCTCAACAA GGAGAAGGAG ATGCCTGTGG AAGGACAGGA TCAACAGACA
    GATTCCACCA AAGGGCGCTG TCTGAGGAGA ACTGTCAGTG TTCCTTCAGA GGGTCAGTTT
    CCGGAGTACC CACAGGAGGG CACCACTAAA CTAGAGGTGC CAGCAGAAAG GTCCCCCCGC
    AGACGGAGCA TCTCAGGGAC CAGCACATCA GAGAAACCCA ACTCCATGGA CACTGCGAAT
    ACCTCTCCTT TTAAAGTACC AGGATTCTTC AGCAAGCGCC TGAAAGGTTC CATCAAGAGG
    ACCAAAAGCC AGTCGAAGCT TGACAGAAAC ACAAGCTTTC GGCTTCCATC CCTTCGCAAT
    GCAGATGACA GGTCTCGAGG TCTGCCTAAA CTGAAAGAGT CGCGTTCCCA TGAGTCCCTG
    CTGAGCCCAT GCAGTGCAGT GGAGTGTCTG GATCTAGGTA GAGGAGAGCC AGTCTCAGTG
    AAGCCACTGC ACAGTAGCAT CCTCGGACAG GACTTCTGCT TCGAGGTCAC CTACTTAAGT
    GGAAGTAAAT GCTTTAGCTG TAGCTCTGCC TCCGAGAGAG ATAAGTGGAT GGAAAACCTT
    CGAAGGACCG TTCAGCCAAA TAAGGACAAT TGTAGACGAG CTGAAAATGT TCTCCGTTTA
    TGGATCATTG AAGCCAAGGA CCTTGCCCCT AAAAAAAAAT ATTTCTGCGA ACTGTGCCTT
    GATGATACCC TCTTTGCTCG TACAACCAGC AAGACCAAAG CAGACAATAT TTTTTGGGGT
    GAACATTTTG AATTCTATAG CCTTCCACCT CTTCATAGCA TCACGGTTCA TATTTATAAG
    GATGTGGAAA AAAAGAAAAA AAAGGACAAG AATAATTATG TAGGGCTAGT CAATATCCCC
    ACTGCCAGTG TGACTGGTCG CCAGTTTGTA GAAAAGTGGT ACCCAGTGAG TACACCCACA
    CCCAACAAAG GAAAGACAGG AGGACCTTCT ATTCGGATTA AGTCACGTTT CCAAACTATC
    ACCATTCTGC CTATGGAGCA ATACAAAGAG TTTGCAGAAT TCATCACCAG CAACTACACC
    ATGCTGTGTT CTGTTCTTGA GCCAGTAATT AGTGTGAGGA ATAAAGAGGA GTTGGCTTGT
    GCCTTAGTAC ACATTCTTCA AAGTACTGGC AGAGCCAAGG ATTTTCTGAC TGACTTGGTG
    ATGTCAGAGG TAGATCGTTG TGGAGAACAT GATGTCTTGA TCTTCAGAGA AAACACCATT
    GCCACTAAAT CCATTGAGGA ATACCTCAAG TTGGTGGGAC AACAGTACCT TCATGATGCA
    CTCGGGGAGT TCATCAAAGC CTTGTATGAA TCAGATGAGA ACTGTGAAGT GGATCCCAGC
    AAGTGTTCAT CTAGTGAACT GATGGACCAT CAGAGCAACC TGAAAATGTG CTGTGAGTTG
    GCTTTCTGCA AGATCATCAA CTCATACTGT GTCTTCCCGC GTGAGTTGAA AGAAGTGTTT
    GCATCATGGA AGCAGCAGTG CCTGAACCGA GGCAAGCAAG ACATCAGTGA GCGGCTCATC
    AGTGCCTCAT TGTTTCTCCG TTTTCTTTGC CCAGCCATTA TGTCTCCCAG TCTTTTCAAC
    CTGATGCAGG AATATCCTGA TGACCGCACA TCTCGGACAC TAACTCTTAT TGCCAAGGTC
    ATTCAGAACC TGGCCAACTT TGCCAAGTTT GGTAACAAAG AGGAGTACAT GGCATTCATG
    AATGATTTTT TAGAACATGA ATGGGGTGGA ATGAAGCGGT TCCTTTTGGA GATCTCTAAT
    CCTGACACCA TCTCAAACAC CCCAGGCTTC GATGGTTACA TTGATTTGGG CAGAGAGCTG
    TCAGTTTTGC ATTCCTTACT ATGGGAAGTA GTTTCCCAAC TTGATAAGGC AACTGTGGCA
    AAACTGGGAC CTCTCCCTCG TGTTCTTGCT GACATTACCA AGTCTCTGAC TAATCCTACG
    CCAATACAGC AGCAGCTAAG ACGTTTCGCT GAGCACAGCT CCAGTCCAAA CGTCAGCGGA
    AGCCTCTCCT CTGGGCTGCA GAGAATATGT GAAGACCCCA CTGACAGTGA TTTGCATAAA
    CTAAAGTCTC CAAGCCAGGA TAACACAGAC AGCTATTTCA GAGGAAAGAC GTTATTGTTG
    GTTCAGCAAG CCTCCTCCCA GAGCATGACG TACTCTGAAA AGGATGAAAA GGAAAACAGC
    CTTCCTAACG GTCGGAGCAT CTCCCTCATG GACCTCCAGG ACACTCATGC TGCTCAGGCG
    GAGCATGCAT CTGTCATGCT TGATGTGCCT ATGCGCCTTG CAGGAAGCCA GCTTTCTATA
    ACCCAGGTAG CTAGCATCAA ACAGCTGCGG GAAACCCAGA GCACTCCCCA GAGTGCACCT
    CAAGTGAGAA GACCCCTGCA CCCAGCCTTG AACCAGCCAG GAAGTCTCCA GCCCTTGTCA
    TTCCAGAATC CTGTCTATCA CCTCAACAAC CCAGTTCCAG CAATGCCAAA GGCCTCTGCG
    GATTCCAGTT TGGAGAACCT AAGCACTGCC AGTTCTAGAA GCCAAAGCAA TAGTGAAGAC
    TTCAAACTCA GTGGACCCAG CAATAGCAGC ATGGAAGATT TCACCAAAAG AAGCTCCCAC
    AGTGAAGACT TCTCCAGGCG CCACACTGTA CCAGACAGAC ACATACCTCT TGCTCTACCA
    CGACAAAATA GTACGGGGCA GTCCCAAATC CGAAAACTGG ACCATTCTGG GTTAGGTGCC
    CGAGCCAAAG CACCCCCATC TCTGCCACAC AGTGCTTCCT TGCGTAGCAC AGGGAGCATG
    TCAGTGGCGT CTGCAGCCCT AATGGCTGAA CCTGTGCAGA ACGGGAGCCG GTCCAGGCAG
    CAGTCCTCTT CCTCCAGAGA GAGCCCTGTT CCCAAAGTGA GAGCAATCCA GAGACAACAG
    ACACAACAGG TTCAGTCACC TGTGGACTCC GCTACAATGT CCCCAGTAGA AAGGACAGCA
    GCCTGGGTTC TGAACAATGG ACAGTATGAA GAGGATGTGG AAGAAACTGA ACAAAATCAA
    GATGAAGCCA AGCATGCTGA AAAGTATGAA CAAGAAATTA CCAAGCTGAA GGAGCGCCTA
    CGAGTGTCCA GCCGGCGGTT GGAGGAGTAT GAGCGCCGGC TGCTGGTGCA GGAGCAGCAG
    ATGCAGAAGC TGCTGCTGGA GTACAAGGCC CGGCTGGAGG ATAGTGAGGA GCGGCTGCGC
    AGACAGCAGG AGGAGAAGGA CAGCCAGATG AAAAGCATCA TCAGCAGGCT AATGGCTGTG
    GAAGAAGAAT TAAAGAAAGA TCATGCTGAG ATGCAAGCAG TTATTGATGC AAAGCAGAAA
    ATAATTGATG CACAGGTCAT AAATGGAAAT GAGATTATTC AAACAGGAAA AGCGGATCGT
    GTCCCTGGAT TCAGCCAACA CGAGACTGAT GAGTGCGCTG ACACAAGTGA AGGAGCGGTA
    CAGCATGCAG GTCCGCAATG GCATCTCTCC CACCAACCCC ACCAAGCTTT CCATCACGGA
    GAATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006496814.1
    CDS284..4132
    Translation

    Target ORF information:

    RefSeq Version XM_006496751.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus RAS protein activator like 2 (Rasal2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006496751.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCTGT CTCCGTCGTC CGGAGGAGCC GCGGAGGCGC TGTCGTGGCC GGAGATGTTC 
    ACGGGGCTGG AGTCCGACTC GCCGCTGCCC CCGGAGGATC TGGACGCGGT CGTCCCAGTC
    AGCGGAGCAG TGGCCGGTGG CATGCTGGAT CGGATCCTTC TGGAGTCAGT GTGCCAGCAG
    CAGAGCTGGG TCCGGGTGTT CGATGTAAAA GGCCCACCAA CCCACCGTCT GTCTTGTGGT
    CAGTCACCCT ATACTGAGAC GACATCATGG GAGAGGAAGT ACTGCATCCT CACAGACAGC
    CAGTTGGTGT TGCTCAACAA GGAGAAGGAG ATGCCTGTGG AAGGACAGGA TCAACAGACA
    GATTCCACCA AAGGGCGCTG TCTGAGGAGA ACTGTCAGTG TTCCTTCAGA GGGTCAGTTT
    CCGGAGTACC CACAGGAGGG CACCACTAAA CTAGAGGTGC CAGCAGAAAG GTCCCCCCGC
    AGACGGAGCA TCTCAGGGAC CAGCACATCA GAGAAACCCA ACTCCATGGA CACTGCGAAT
    ACCTCTCCTT TTAAAGTACC AGGATTCTTC AGCAAGCGCC TGAAAGGTTC CATCAAGAGG
    ACCAAAAGCC AGTCGAAGCT TGACAGAAAC ACAAGCTTTC GGCTTCCATC CCTTCGCAAT
    GCAGATGACA GGTCTCGAGG TCTGCCTAAA CTGAAAGAGT CGCGTTCCCA TGAGTCCCTG
    CTGAGCCCAT GCAGTGCAGT GGAGTGTCTG GATCTAGGTA GAGGAGAGCC AGTCTCAGTG
    AAGCCACTGC ACAGTAGCAT CCTCGGACAG GACTTCTGCT TCGAGGTCAC CTACTTAAGT
    GGAAGTAAAT GCTTTAGCTG TAGCTCTGCC TCCGAGAGAG ATAAGTGGAT GGAAAACCTT
    CGAAGGACCG TTCAGCCAAA TAAGGACAAT TGTAGACGAG CTGAAAATGT TCTCCGTTTA
    TGGATCATTG AAGCCAAGGA CCTTGCCCCT AAAAAAAAAT ATTTCTGCGA ACTGTGCCTT
    GATGATACCC TCTTTGCTCG TACAACCAGC AAGACCAAAG CAGACAATAT TTTTTGGGGT
    GAACATTTTG AATTCTATAG CCTTCCACCT CTTCATAGCA TCACGGTTCA TATTTATAAG
    GATGTGGAAA AAAAGAAAAA AAAGGACAAG AATAATTATG TAGGGCTAGT CAATATCCCC
    ACTGCCAGTG TGACTGGTCG CCAGTTTGTA GAAAAGTGGT ACCCAGTGAG TACACCCACA
    CCCAACAAAG GAAAGACAGG AGGACCTTCT ATTCGGATTA AGTCACGTTT CCAAACTATC
    ACCATTCTGC CTATGGAGCA ATACAAAGAG TTTGCAGAAT TCATCACCAG CAACTACACC
    ATGCTGTGTT CTGTTCTTGA GCCAGTAATT AGTGTGAGGA ATAAAGAGGA GTTGGCTTGT
    GCCTTAGTAC ACATTCTTCA AAGTACTGGC AGAGCCAAGG ATTTTCTGAC TGACTTGGTG
    ATGTCAGAGG TAGATCGTTG TGGAGAACAT GATGTCTTGA TCTTCAGAGA AAACACCATT
    GCCACTAAAT CCATTGAGGA ATACCTCAAG TTGGTGGGAC AACAGTACCT TCATGATGCA
    CTCGGGGAGT TCATCAAAGC CTTGTATGAA TCAGATGAGA ACTGTGAAGT GGATCCCAGC
    AAGTGTTCAT CTAGTGAACT GATGGACCAT CAGAGCAACC TGAAAATGTG CTGTGAGTTG
    GCTTTCTGCA AGATCATCAA CTCATACTGT GTCTTCCCGC GTGAGTTGAA AGAAGTGTTT
    GCATCATGGA AGCAGCAGTG CCTGAACCGA GGCAAGCAAG ACATCAGTGA GCGGCTCATC
    AGTGCCTCAT TGTTTCTCCG TTTTCTTTGC CCAGCCATTA TGTCTCCCAG TCTTTTCAAC
    CTGATGCAGG AATATCCTGA TGACCGCACA TCTCGGACAC TAACTCTTAT TGCCAAGGTC
    ATTCAGAACC TGGCCAACTT TGCCAAGTTT GGTAACAAAG AGGAGTACAT GGCATTCATG
    AATGATTTTT TAGAACATGA ATGGGGTGGA ATGAAGCGGT TCCTTTTGGA GATCTCTAAT
    CCTGACACCA TCTCAAACAC CCCAGGCTTC GATGGTTACA TTGATTTGGG CAGAGAGCTG
    TCAGTTTTGC ATTCCTTACT ATGGGAAGTA GTTTCCCAAC TTGATAAGGC AACTGTGGCA
    AAACTGGGAC CTCTCCCTCG TGTTCTTGCT GACATTACCA AGTCTCTGAC TAATCCTACG
    CCAATACAGC AGCAGCTAAG ACGTTTCGCT GAGCACAGCT CCAGTCCAAA CGTCAGCGGA
    AGCCTCTCCT CTGGGCTGCA GAGAATATGT GAAGACCCCA CTGACAGTGA TTTGCATAAA
    CTAAAGTCTC CAAGCCAGGA TAACACAGAC AGCTATTTCA GAGGAAAGAC GTTATTGTTG
    GTTCAGCAAG CCTCCTCCCA GAGCATGACG TACTCTGAAA AGGATGAAAA GGAAAACAGC
    CTTCCTAACG GTCGGAGCAT CTCCCTCATG GACCTCCAGG ACACTCATGC TGCTCAGGCG
    GAGCATGCAT CTGTCATGCT TGATGTGCCT ATGCGCCTTG CAGGAAGCCA GCTTTCTATA
    ACCCAGGTAG CTAGCATCAA ACAGCTGCGG GAAACCCAGA GCACTCCCCA GAGTGCACCT
    CAAGTGAGAA GACCCCTGCA CCCAGCCTTG AACCAGCCAG GAAGTCTCCA GCCCTTGTCA
    TTCCAGAATC CTGTCTATCA CCTCAACAAC CCAGTTCCAG CAATGCCAAA GGCCTCTGCG
    GATTCCAGTT TGGAGAACCT AAGCACTGCC AGTTCTAGAA GCCAAAGCAA TAGTGAAGAC
    TTCAAACTCA GTGGACCCAG CAATAGCAGC ATGGAAGATT TCACCAAAAG AAGCTCCCAC
    AGTGAAGACT TCTCCAGGCG CCACACTGTA CCAGACAGAC ACATACCTCT TGCTCTACCA
    CGACAAAATA GTACGGGGCA GTCCCAAATC CGAAAACTGG ACCATTCTGG GTTAGGTGCC
    CGAGCCAAAG CACCCCCATC TCTGCCACAC AGTGCTTCCT TGCGTAGCAC AGGGAGCATG
    TCAGTGGCGT CTGCAGCCCT AATGGCTGAA CCTGTGCAGA ACGGGAGCCG GTCCAGGCAG
    CAGTCCTCTT CCTCCAGAGA GAGCCCTGTT CCCAAAGTGA GAGCAATCCA GAGACAACAG
    ACACAACAGG TTCAGTCACC TGTGGACTCC GCTACAATGT CCCCAGTAGA AAGGACAGCA
    GCCTGGGTTC TGAACAATGG ACAGTATGAA GAGGATGTGG AAGAAACTGA ACAAAATCAA
    GATGAAGCCA AGCATGCTGA AAAGTATGAA CAAGAAATTA CCAAGCTGAA GGAGCGCCTA
    CGAGTGTCCA GCCGGCGGTT GGAGGAGTAT GAGCGCCGGC TGCTGGTGCA GGAGCAGCAG
    ATGCAGAAGC TGCTGCTGGA GTACAAGGCC CGGCTGGAGG ATAGTGAGGA GCGGCTGCGC
    AGACAGCAGG AGGAGAAGGA CAGCCAGATG AAAAGCATCA TCAGCAGGCT AATGGCTGTG
    GAAGAAGAAT TAAAGAAAGA TCATGCTGAG ATGCAAGCAG TTATTGATGC AAAGCAGAAA
    ATAATTGATG CACAGGTCAT AAATGGAAAT GAGATTATTC AAACAGGAAA AGCGGATCGT
    GTCCCTGGAT TCAGCCAACA CGAGACTGAT GAGTGCGCTG ACACAAGTGA AGGAGCGGTA
    CAGCATGCAG GTCCGCAATG GCATCTCTCC CACCAACCCC ACCAAGCTTT CCATCACGGA
    GAATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu28683
    Clone ID Related Accession (Same CDS sequence) XM_006496752.1 , XM_006496752.2
    Accession Version XM_006496752.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3840bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product ras GTPase-activating protein nGAP isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGCTGT CTCCGTCGTC CGGAGGAGCC GCGGAGGCGC TGTCGTGGCC GGAGATGTTC 
    ACGGGGCTGG AGTCCGACTC GCCGCTGCCC CCGGAGGATC TGGACGCGGT CGTCCCAGTC
    AGCGGAGCAG TGGCCGGTGG CATGCTGGAT CGGATCCTTC TGGAGTCAGT GTGCCAGCAG
    CAGAGCTGGG TCCGGGTGTT CGATGTAAAA GGCCCACCAA CCCACCGTCT GTCTTGTGGT
    CAGTCACCCT ATACTGAGAC GACATCATGG GAGAGGAAGT ACTGCATCCT CACAGACAGC
    CAGTTGGTGT TGCTCAACAA GGAGAAGGAG ATGCCTGTGG AAGGACAGGA TCAACAGACA
    GATTCCACCA AAGGGCGCTG TCTGAGGAGA ACTGTCAGTG TTCCTTCAGA GGGTCAGTTT
    CCGGAGTACC CACAGGAGGG CACCACTAAA CTAGAGGTGC CAGCAGAAAG GTCCCCCCGC
    AGACGGAGCA TCTCAGGGAC CAGCACATCA GAGAAACCCA ACTCCATGGA CACTGCGAAT
    ACCTCTCCTT TTAAAGTACC AGGATTCTTC AGCAAGCGCC TGAAAGGTTC CATCAAGAGG
    ACCAAAAGCC AGTCGAAGCT TGACAGAAAC ACAAGCTTTC GGCTTCCATC CCTTCGCAAT
    GCAGATGACA GGTCTCGAGG TCTGCCTAAA CTGAAAGAGT CGCGTTCCCA TGAGTCCCTG
    CTGAGCCCAT GCAGTGCAGT GGAGTGTCTG GATCTAGGTA GAGGAGAGCC AGTCTCAGTG
    AAGCCACTGC ACAGTAGCAT CCTCGGACAG GACTTCTGCT TCGAGGTCAC CTACTTAAGT
    GGAAGTAAAT GCTTTAGCTG TAGCTCTGCC TCCGAGAGAG ATAAGTGGAT GGAAAACCTT
    CGAAGGACCG TTCAGCCAAA TAAGGACAAT TGTAGACGAG CTGAAAATGT TCTCCGTTTA
    TGGATCATTG AAGCCAAGGA CCTTGCCCCT AAAAAAAAAT ATTTCTGCGA ACTGTGCCTT
    GATGATACCC TCTTTGCTCG TACAACCAGC AAGACCAAAG CAGACAATAT TTTTTGGGGT
    GAACATTTTG AATTCTATAG CCTTCCACCT CTTCATAGCA TCACGGTTCA TATTTATAAG
    GATGTGGAAA AAAAGAAAAA AAAGGACAAG AATAATTATG TAGGGCTAGT CAATATCCCC
    ACTGCCAGTG TGACTGGTCG CCAGTTTGTA GAAAAGTGGT ACCCAGTGAG TACACCCACA
    CCCAACAAAG GAAAGACAGG AGGACCTTCT ATTCGGATTA AGTCACGTTT CCAAACTATC
    ACCATTCTGC CTATGGAGCA ATACAAAGAG TTTGCAGAAT TCATCACCAG CAACTACACC
    ATGCTGTGTT CTGTTCTTGA GCCAGTAATT AGTGTGAGGA ATAAAGAGGA GTTGGCTTGT
    GCCTTAGTAC ACATTCTTCA AAGTACTGGC AGAGCCAAGG ATTTTCTGAC TGACTTGGTG
    ATGTCAGAGG TAGATCGTTG TGGAGAACAT GATGTCTTGA TCTTCAGAGA AAACACCATT
    GCCACTAAAT CCATTGAGGA ATACCTCAAG TTGGTGGGAC AACAGTACCT TCATGATGCA
    CTCGGGGAGT TCATCAAAGC CTTGTATGAA TCAGATGAGA ACTGTGAAGT GGATCCCAGC
    AAGTGTTCAT CTAGTGAACT GATGGACCAT CAGAGCAACC TGAAAATGTG CTGTGAGTTG
    GCTTTCTGCA AGATCATCAA CTCATACTGT GTCTTCCCGC GTGAGTTGAA AGAAGTGTTT
    GCATCATGGA AGCAGCAGTG CCTGAACCGA GGCAAGCAAG ACATCAGTGA GCGGCTCATC
    AGTGCCTCAT TGTTTCTCCG TTTTCTTTGC CCAGCCATTA TGTCTCCCAG TCTTTTCAAC
    CTGATGCAGG AATATCCTGA TGACCGCACA TCTCGGACAC TAACTCTTAT TGCCAAGGTC
    ATTCAGAACC TGGCCAACTT TGCCAAGTTT GGTAACAAAG AGGAGTACAT GGCATTCATG
    AATGATTTTT TAGAACATGA ATGGGGTGGA ATGAAGCGGT TCCTTTTGGA GATCTCTAAT
    CCTGACACCA TCTCAAACAC CCCAGGCTTC GATGGTTACA TTGATTTGGG CAGAGAGCTG
    TCAGTTTTGC ATTCCTTACT ATGGGAAGTA GTTTCCCAAC TTGATAAGGC AACTGTGGCA
    AAACTGGGAC CTCTCCCTCG TGTTCTTGCT GACATTACCA AGTCTCTGAC TAATCCTACG
    CCAATACAGC AGCAGCTAAG ACGTTTCGCT GAGCACAGCT CCAGTCCAAA CGTCAGCGGA
    AGCCTCTCCT CTGGGCTGCA GAGAATATGT GAAGACCCCA CTGACAGTGA TTTGCATAAA
    CTAAAGTCTC CAAGCCAGGA TAACACAGAC AGCTATTTCA GAGGAAAGAC GTTATTGTTG
    GTTCAGCAAG CCTCCTCCCA GAGCATGACG TACTCTGAAA AGGATGAAAA GGAAAACAGC
    CTTCCTAACG GTCGGAGCAT CTCCCTCATG GACCTCCAGG ACACTCATGC TGCTCAGGCG
    GAGCATGCAT CTGTCATGCT TGATGTGCCT ATGCGCCTTG CAGGAAGCCA GCTTTCTATA
    ACCCAGGTAG CTAGCATCAA ACAGCTGCGG GAAACCCAGA GCACTCCCCA GAGTGCACCT
    CAAGTGAGAA GACCCCTGCA CCCAGCCTTG AACCAGCCAG GAAGTCTCCA GCCCTTGTCA
    TTCCAGAATC CTGTCTATCA CCTCAACAAC CCAGTTCCAG CAATGCCAAA GGCCTCTGCG
    GATTCCAGTT TGGAGAACCT AAGCACTGCC AGTTCTAGAA GCCAAAGCAA TAGTGAAGAC
    TTCAAACTCA GTGGACCCAG CAATAGCAGC ATGGAAGATT TCACCAAAAG AAGCTCCCAC
    AGTGAAGACT TCTCCAGGCG CCACACTGTA CCAGACAGAC ACATACCTCT TGCTCTACCA
    CGACAAAATA GTACGGGGCA GTCCCAAATC CGAAAACTGG ACCATTCTGG GTTAGGTGCC
    CGAGCCAAAG CACCCCCATC TCTGCCACAC AGTGCTTCCT TGCGTAGCAC AGGGAGCATG
    TCAGTGGCGT CTGCAGCCCT AATGGCTGAA CCTGTGCAGA ACGGGAGCCG GTCCAGGCAG
    CAGTCCTCTT CCTCCAGAGA GAGCCCTGTT CCCAAAGTGA GAGCAATCCA GAGACAACAG
    ACACAACAGG TTCAGTCACC TGTGGACTCC GCTACAATGT CCCCAGTAGA AAGGACAGCA
    GCCTGGGTTC TGAACAATGG ACAGTATGAA GAGGATGTGG AAGAAACTGA ACAAAATCAA
    GATGAAGCCA AGCATGCTGA AAAGTATGAA CAAGAAATTA CCAAGCTGAA GGAGCGCCTA
    CGAGTGTCCA GCCGGCGGTT GGAGGAGTAT GAGCGCCGGC TGCTGGTGCA GGAGCAGCAG
    ATGCAGAAGC TGCTGCTGGA GTACAAGGCC CGGCTGGAGG ATAGTGAGGA GCGGCTGCGC
    AGACAGCAGG AGGAGAAGGA CAGCCAGATG AAAAGCATCA TCAGCAGGCT AATGGCTGTG
    GAAGAAGAAT TAAAGAAAGA TCATGCTGAG ATGCAAGCAG TTATTGATGC AAAGCAGAAA
    ATAATTGATG CACAGGAAAA GCGGATCGTG TCCCTGGATT CAGCCAACAC GAGACTGATG
    AGTGCGCTGA CACAAGTGAA GGAGCGGTAC AGCATGCAGG TCCGCAATGG CATCTCTCCC
    ACCAACCCCA CCAAGCTTTC CATCACGGAG AATGGTGAAT TCAAAAATAG CAGCTGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006496815.1
    CDS284..4123
    Translation

    Target ORF information:

    RefSeq Version XM_006496752.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus RAS protein activator like 2 (Rasal2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006496752.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGCTGT CTCCGTCGTC CGGAGGAGCC GCGGAGGCGC TGTCGTGGCC GGAGATGTTC 
    ACGGGGCTGG AGTCCGACTC GCCGCTGCCC CCGGAGGATC TGGACGCGGT CGTCCCAGTC
    AGCGGAGCAG TGGCCGGTGG CATGCTGGAT CGGATCCTTC TGGAGTCAGT GTGCCAGCAG
    CAGAGCTGGG TCCGGGTGTT CGATGTAAAA GGCCCACCAA CCCACCGTCT GTCTTGTGGT
    CAGTCACCCT ATACTGAGAC GACATCATGG GAGAGGAAGT ACTGCATCCT CACAGACAGC
    CAGTTGGTGT TGCTCAACAA GGAGAAGGAG ATGCCTGTGG AAGGACAGGA TCAACAGACA
    GATTCCACCA AAGGGCGCTG TCTGAGGAGA ACTGTCAGTG TTCCTTCAGA GGGTCAGTTT
    CCGGAGTACC CACAGGAGGG CACCACTAAA CTAGAGGTGC CAGCAGAAAG GTCCCCCCGC
    AGACGGAGCA TCTCAGGGAC CAGCACATCA GAGAAACCCA ACTCCATGGA CACTGCGAAT
    ACCTCTCCTT TTAAAGTACC AGGATTCTTC AGCAAGCGCC TGAAAGGTTC CATCAAGAGG
    ACCAAAAGCC AGTCGAAGCT TGACAGAAAC ACAAGCTTTC GGCTTCCATC CCTTCGCAAT
    GCAGATGACA GGTCTCGAGG TCTGCCTAAA CTGAAAGAGT CGCGTTCCCA TGAGTCCCTG
    CTGAGCCCAT GCAGTGCAGT GGAGTGTCTG GATCTAGGTA GAGGAGAGCC AGTCTCAGTG
    AAGCCACTGC ACAGTAGCAT CCTCGGACAG GACTTCTGCT TCGAGGTCAC CTACTTAAGT
    GGAAGTAAAT GCTTTAGCTG TAGCTCTGCC TCCGAGAGAG ATAAGTGGAT GGAAAACCTT
    CGAAGGACCG TTCAGCCAAA TAAGGACAAT TGTAGACGAG CTGAAAATGT TCTCCGTTTA
    TGGATCATTG AAGCCAAGGA CCTTGCCCCT AAAAAAAAAT ATTTCTGCGA ACTGTGCCTT
    GATGATACCC TCTTTGCTCG TACAACCAGC AAGACCAAAG CAGACAATAT TTTTTGGGGT
    GAACATTTTG AATTCTATAG CCTTCCACCT CTTCATAGCA TCACGGTTCA TATTTATAAG
    GATGTGGAAA AAAAGAAAAA AAAGGACAAG AATAATTATG TAGGGCTAGT CAATATCCCC
    ACTGCCAGTG TGACTGGTCG CCAGTTTGTA GAAAAGTGGT ACCCAGTGAG TACACCCACA
    CCCAACAAAG GAAAGACAGG AGGACCTTCT ATTCGGATTA AGTCACGTTT CCAAACTATC
    ACCATTCTGC CTATGGAGCA ATACAAAGAG TTTGCAGAAT TCATCACCAG CAACTACACC
    ATGCTGTGTT CTGTTCTTGA GCCAGTAATT AGTGTGAGGA ATAAAGAGGA GTTGGCTTGT
    GCCTTAGTAC ACATTCTTCA AAGTACTGGC AGAGCCAAGG ATTTTCTGAC TGACTTGGTG
    ATGTCAGAGG TAGATCGTTG TGGAGAACAT GATGTCTTGA TCTTCAGAGA AAACACCATT
    GCCACTAAAT CCATTGAGGA ATACCTCAAG TTGGTGGGAC AACAGTACCT TCATGATGCA
    CTCGGGGAGT TCATCAAAGC CTTGTATGAA TCAGATGAGA ACTGTGAAGT GGATCCCAGC
    AAGTGTTCAT CTAGTGAACT GATGGACCAT CAGAGCAACC TGAAAATGTG CTGTGAGTTG
    GCTTTCTGCA AGATCATCAA CTCATACTGT GTCTTCCCGC GTGAGTTGAA AGAAGTGTTT
    GCATCATGGA AGCAGCAGTG CCTGAACCGA GGCAAGCAAG ACATCAGTGA GCGGCTCATC
    AGTGCCTCAT TGTTTCTCCG TTTTCTTTGC CCAGCCATTA TGTCTCCCAG TCTTTTCAAC
    CTGATGCAGG AATATCCTGA TGACCGCACA TCTCGGACAC TAACTCTTAT TGCCAAGGTC
    ATTCAGAACC TGGCCAACTT TGCCAAGTTT GGTAACAAAG AGGAGTACAT GGCATTCATG
    AATGATTTTT TAGAACATGA ATGGGGTGGA ATGAAGCGGT TCCTTTTGGA GATCTCTAAT
    CCTGACACCA TCTCAAACAC CCCAGGCTTC GATGGTTACA TTGATTTGGG CAGAGAGCTG
    TCAGTTTTGC ATTCCTTACT ATGGGAAGTA GTTTCCCAAC TTGATAAGGC AACTGTGGCA
    AAACTGGGAC CTCTCCCTCG TGTTCTTGCT GACATTACCA AGTCTCTGAC TAATCCTACG
    CCAATACAGC AGCAGCTAAG ACGTTTCGCT GAGCACAGCT CCAGTCCAAA CGTCAGCGGA
    AGCCTCTCCT CTGGGCTGCA GAGAATATGT GAAGACCCCA CTGACAGTGA TTTGCATAAA
    CTAAAGTCTC CAAGCCAGGA TAACACAGAC AGCTATTTCA GAGGAAAGAC GTTATTGTTG
    GTTCAGCAAG CCTCCTCCCA GAGCATGACG TACTCTGAAA AGGATGAAAA GGAAAACAGC
    CTTCCTAACG GTCGGAGCAT CTCCCTCATG GACCTCCAGG ACACTCATGC TGCTCAGGCG
    GAGCATGCAT CTGTCATGCT TGATGTGCCT ATGCGCCTTG CAGGAAGCCA GCTTTCTATA
    ACCCAGGTAG CTAGCATCAA ACAGCTGCGG GAAACCCAGA GCACTCCCCA GAGTGCACCT
    CAAGTGAGAA GACCCCTGCA CCCAGCCTTG AACCAGCCAG GAAGTCTCCA GCCCTTGTCA
    TTCCAGAATC CTGTCTATCA CCTCAACAAC CCAGTTCCAG CAATGCCAAA GGCCTCTGCG
    GATTCCAGTT TGGAGAACCT AAGCACTGCC AGTTCTAGAA GCCAAAGCAA TAGTGAAGAC
    TTCAAACTCA GTGGACCCAG CAATAGCAGC ATGGAAGATT TCACCAAAAG AAGCTCCCAC
    AGTGAAGACT TCTCCAGGCG CCACACTGTA CCAGACAGAC ACATACCTCT TGCTCTACCA
    CGACAAAATA GTACGGGGCA GTCCCAAATC CGAAAACTGG ACCATTCTGG GTTAGGTGCC
    CGAGCCAAAG CACCCCCATC TCTGCCACAC AGTGCTTCCT TGCGTAGCAC AGGGAGCATG
    TCAGTGGCGT CTGCAGCCCT AATGGCTGAA CCTGTGCAGA ACGGGAGCCG GTCCAGGCAG
    CAGTCCTCTT CCTCCAGAGA GAGCCCTGTT CCCAAAGTGA GAGCAATCCA GAGACAACAG
    ACACAACAGG TTCAGTCACC TGTGGACTCC GCTACAATGT CCCCAGTAGA AAGGACAGCA
    GCCTGGGTTC TGAACAATGG ACAGTATGAA GAGGATGTGG AAGAAACTGA ACAAAATCAA
    GATGAAGCCA AGCATGCTGA AAAGTATGAA CAAGAAATTA CCAAGCTGAA GGAGCGCCTA
    CGAGTGTCCA GCCGGCGGTT GGAGGAGTAT GAGCGCCGGC TGCTGGTGCA GGAGCAGCAG
    ATGCAGAAGC TGCTGCTGGA GTACAAGGCC CGGCTGGAGG ATAGTGAGGA GCGGCTGCGC
    AGACAGCAGG AGGAGAAGGA CAGCCAGATG AAAAGCATCA TCAGCAGGCT AATGGCTGTG
    GAAGAAGAAT TAAAGAAAGA TCATGCTGAG ATGCAAGCAG TTATTGATGC AAAGCAGAAA
    ATAATTGATG CACAGGAAAA GCGGATCGTG TCCCTGGATT CAGCCAACAC GAGACTGATG
    AGTGCGCTGA CACAAGTGAA GGAGCGGTAC AGCATGCAGG TCCGCAATGG CATCTCTCCC
    ACCAACCCCA CCAAGCTTTC CATCACGGAG AATGGTGAAT TCAAAAATAG CAGCTGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu28684
    Clone ID Related Accession (Same CDS sequence) XM_006496753.4 , XM_006496753.3
    Accession Version XM_006496753.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3678bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product ras GTPase-activating protein nGAP isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006496753.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGTACAGA GACAGAATTC AGAGTGGAGG GAGGATCACA TTTTCTTTCA TCATTACAGC 
    CTAATCCACA ATTTTAGTAC TGAACCCCGC CTCCTCCAAG AATCTAGTCT CTTTATCCAT
    TTCTTCCCAC TTCATCATTC AGTAATTGTA TTCTGTATGG AAAAGTTTTC TGGCTGGAGA
    ATGAGTCTCC TTTCCCCTGG TGATGTGTCG GAGTGGTTGG ATAGTAGCAC CGAAGAGGAG
    AAGAGCCTTC TGTCATTTCT GGAGGTGCCA GCAGAAAGGT CCCCCCGCAG ACGGAGCATC
    TCAGGGACCA GCACATCAGA GAAACCCAAC TCCATGGACA CTGCGAATAC CTCTCCTTTT
    AAAGTACCAG GATTCTTCAG CAAGCGCCTG AAAGGTTCCA TCAAGAGGAC CAAAAGCCAG
    TCGAAGCTTG ACAGAAACAC AAGCTTTCGG CTTCCATCCC TTCGCAATGC AGATGACAGG
    TCTCGAGGTC TGCCTAAACT GAAAGAGTCG CGTTCCCATG AGTCCCTGCT GAGCCCATGC
    AGTGCAGTGG AGTGTCTGGA TCTAGGTAGA GGAGAGCCAG TCTCAGTGAA GCCACTGCAC
    AGTAGCATCC TCGGACAGGA CTTCTGCTTC GAGGTCACCT ACTTAAGTGG AAGTAAATGC
    TTTAGCTGTA GCTCTGCCTC CGAGAGAGAT AAGTGGATGG AAAACCTTCG AAGGACCGTT
    CAGCCAAATA AGGACAATTG TAGACGAGCT GAAAATGTTC TCCGTTTATG GATCATTGAA
    GCCAAGGACC TTGCCCCTAA AAAAAAATAT TTCTGCGAAC TGTGCCTTGA TGATACCCTC
    TTTGCTCGTA CAACCAGCAA GACCAAAGCA GACAATATTT TTTGGGGTGA ACATTTTGAA
    TTCTATAGCC TTCCACCTCT TCATAGCATC ACGGTTCATA TTTATAAGGA TGTGGAAAAA
    AAGAAAAAAA AGGACAAGAA TAATTATGTA GGGCTAGTCA ATATCCCCAC TGCCAGTGTG
    ACTGGTCGCC AGTTTGTAGA AAAGTGGTAC CCAGTGAGTA CACCCACACC CAACAAAGGA
    AAGACAGGAG GACCTTCTAT TCGGATTAAG TCACGTTTCC AAACTATCAC CATTCTGCCT
    ATGGAGCAAT ACAAAGAGTT TGCAGAATTC ATCACCAGCA ACTACACCAT GCTGTGTTCT
    GTTCTTGAGC CAGTAATTAG TGTGAGGAAT AAAGAGGAGT TGGCTTGTGC CTTAGTACAC
    ATTCTTCAAA GTACTGGCAG AGCCAAGGAT TTTCTGACTG ACTTGGTGAT GTCAGAGGTA
    GATCGTTGTG GAGAACATGA TGTCTTGATC TTCAGAGAAA ACACCATTGC CACTAAATCC
    ATTGAGGAAT ACCTCAAGTT GGTGGGACAA CAGTACCTTC ATGATGCACT CGGGGAGTTC
    ATCAAAGCCT TGTATGAATC AGATGAGAAC TGTGAAGTGG ATCCCAGCAA GTGTTCATCT
    AGTGAACTGA TGGACCATCA GAGCAACCTG AAAATGTGCT GTGAGTTGGC TTTCTGCAAG
    ATCATCAACT CATACTGTGT CTTCCCGCGT GAGTTGAAAG AAGTGTTTGC ATCATGGAAG
    CAGCAGTGCC TGAACCGAGG CAAGCAAGAC ATCAGTGAGC GGCTCATCAG TGCCTCATTG
    TTTCTCCGTT TTCTTTGCCC AGCCATTATG TCTCCCAGTC TTTTCAACCT GATGCAGGAA
    TATCCTGATG ACCGCACATC TCGGACACTA ACTCTTATTG CCAAGGTCAT TCAGAACCTG
    GCCAACTTTG CCAAGTTTGG TAACAAAGAG GAGTACATGG CATTCATGAA TGATTTTTTA
    GAACATGAAT GGGGTGGAAT GAAGCGGTTC CTTTTGGAGA TCTCTAATCC TGACACCATC
    TCAAACACCC CAGGCTTCGA TGGTTACATT GATTTGGGCA GAGAGCTGTC AGTTTTGCAT
    TCCTTACTAT GGGAAGTAGT TTCCCAACTT GATAAGGGTG AAAATTCCTT TCTACAGGCA
    ACTGTGGCAA AACTGGGACC TCTCCCTCGT GTTCTTGCTG ACATTACCAA GTCTCTGACT
    AATCCTACGC CAATACAGCA GCAGCTAAGA CGTTTCGCTG AGCACAGCTC CAGTCCAAAC
    GTCAGCGGAA GCCTCTCCTC TGGGCTGCAG AGAATATGTG AAGACCCCAC TGACAGTGAT
    TTGCATAAAC TAAAGTCTCC AAGCCAGGAT AACACAGACA GCTATTTCAG AGGAAAGACG
    TTATTGTTGG TTCAGCAAGC CTCCTCCCAG AGCATGACGT ACTCTGAAAA GGATGAAAAG
    GAAAACAGCC TTCCTAACGG TCGGAGCATC TCCCTCATGG ACCTCCAGGA CACTCATGCT
    GCTCAGGCGG AGCATGCATC TGTCATGCTT GATGTGCCTA TGCGCCTTGC AGGAAGCCAG
    CTTTCTATAA CCCAGGTAGC TAGCATCAAA CAGCTGCGGG AAACCCAGAG CACTCCCCAG
    AGTGCACCTC AAGTGAGAAG ACCCCTGCAC CCAGCCTTGA ACCAGCCAGG AAGTCTCCAG
    CCCTTGTCAT TCCAGAATCC TGTCTATCAC CTCAACAACC CAGTTCCAGC AATGCCAAAG
    GCCTCTGCGG ATTCCAGTTT GGAGAACCTA AGCACTGCCA GTTCTAGAAG CCAAAGCAAT
    AGTGAAGACT TCAAACTCAG TGGACCCAGC AATAGCAGCA TGGAAGATTT CACCAAAAGA
    AGCTCCCACA GTGAAGACTT CTCCAGGCGC CACACTGTAC CAGACAGACA CATACCTCTT
    GCTCTACCAC GACAAAATAG TACGGGGCAG TCCCAAATCC GAAAACTGGA CCATTCTGGG
    TTAGGTGCCC GAGCCAAAGC ACCCCCATCT CTGCCACACA GTGCTTCCTT GCGTAGCACA
    GGGAGCATGT CAGTGGCGTC TGCAGCCCTA ATGGCTGAAC CTGTGCAGAA CGGGAGCCGG
    TCCAGGCAGC AGTCCTCTTC CTCCAGAGAG AGCCCTGTTC CCAAAGTGAG AGCAATCCAG
    AGACAACAGA CACAACAGGT TCAGTCACCT GTGGACTCCG CTACAATGTC CCCAGTAGAA
    AGGACAGCAG CCTGGGTTCT GAACAATGGA CAGTATGAAG AGGATGTGGA AGAAACTGAA
    CAAAATCAAG ATGAAGCCAA GCATGCTGAA AAGTATGAAC AAGAAATTAC CAAGCTGAAG
    GAGCGCCTAC GAGTGTCCAG CCGGCGGTTG GAGGAGTATG AGCGCCGGCT GCTGGTGCAG
    GAGCAGCAGA TGCAGAAGCT GCTGCTGGAG TACAAGGCCC GGCTGGAGGA TAGTGAGGAG
    CGGCTGCGCA GACAGCAGGA GGAGAAGGAC AGCCAGATGA AAAGCATCAT CAGCAGGCTA
    ATGGCTGTGG AAGAAGAATT AAAGAAAGAT CATGCTGAGA TGCAAGCAGT TATTGATGCA
    AAGCAGAAAA TAATTGATGC ACAGGTCATA AATGGAAATG AGATTATTCA AACAGGAAAA
    GCGGATCGTG TCCCTGGATT CAGCCAACAC GAGACTGATG AGTGCGCTGA CACAAGTGAA
    GGAGCGGTAC AGCATGCAGG TCCGCAATGG CATCTCTCCC ACCAACCCCA CCAAGCTTTC
    CATCACGGAG AATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006496816.1
    CDS1051..4728
    Translation

    Target ORF information:

    RefSeq Version XM_006496753.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus RAS protein activator like 2 (Rasal2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006496753.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGTACAGA GACAGAATTC AGAGTGGAGG GAGGATCACA TTTTCTTTCA TCATTACAGC 
    CTAATCCACA ATTTTAGTAC TGAACCCCGC CTCCTCCAAG AATCTAGTCT CTTTATCCAT
    TTCTTCCCAC TTCATCATTC AGTAATTGTA TTCTGTATGG AAAAGTTTTC TGGCTGGAGA
    ATGAGTCTCC TTTCCCCTGG TGATGTGTCG GAGTGGTTGG ATAGTAGCAC CGAAGAGGAG
    AAGAGCCTTC TGTCATTTCT GGAGGTGCCA GCAGAAAGGT CCCCCCGCAG ACGGAGCATC
    TCAGGGACCA GCACATCAGA GAAACCCAAC TCCATGGACA CTGCGAATAC CTCTCCTTTT
    AAAGTACCAG GATTCTTCAG CAAGCGCCTG AAAGGTTCCA TCAAGAGGAC CAAAAGCCAG
    TCGAAGCTTG ACAGAAACAC AAGCTTTCGG CTTCCATCCC TTCGCAATGC AGATGACAGG
    TCTCGAGGTC TGCCTAAACT GAAAGAGTCG CGTTCCCATG AGTCCCTGCT GAGCCCATGC
    AGTGCAGTGG AGTGTCTGGA TCTAGGTAGA GGAGAGCCAG TCTCAGTGAA GCCACTGCAC
    AGTAGCATCC TCGGACAGGA CTTCTGCTTC GAGGTCACCT ACTTAAGTGG AAGTAAATGC
    TTTAGCTGTA GCTCTGCCTC CGAGAGAGAT AAGTGGATGG AAAACCTTCG AAGGACCGTT
    CAGCCAAATA AGGACAATTG TAGACGAGCT GAAAATGTTC TCCGTTTATG GATCATTGAA
    GCCAAGGACC TTGCCCCTAA AAAAAAATAT TTCTGCGAAC TGTGCCTTGA TGATACCCTC
    TTTGCTCGTA CAACCAGCAA GACCAAAGCA GACAATATTT TTTGGGGTGA ACATTTTGAA
    TTCTATAGCC TTCCACCTCT TCATAGCATC ACGGTTCATA TTTATAAGGA TGTGGAAAAA
    AAGAAAAAAA AGGACAAGAA TAATTATGTA GGGCTAGTCA ATATCCCCAC TGCCAGTGTG
    ACTGGTCGCC AGTTTGTAGA AAAGTGGTAC CCAGTGAGTA CACCCACACC CAACAAAGGA
    AAGACAGGAG GACCTTCTAT TCGGATTAAG TCACGTTTCC AAACTATCAC CATTCTGCCT
    ATGGAGCAAT ACAAAGAGTT TGCAGAATTC ATCACCAGCA ACTACACCAT GCTGTGTTCT
    GTTCTTGAGC CAGTAATTAG TGTGAGGAAT AAAGAGGAGT TGGCTTGTGC CTTAGTACAC
    ATTCTTCAAA GTACTGGCAG AGCCAAGGAT TTTCTGACTG ACTTGGTGAT GTCAGAGGTA
    GATCGTTGTG GAGAACATGA TGTCTTGATC TTCAGAGAAA ACACCATTGC CACTAAATCC
    ATTGAGGAAT ACCTCAAGTT GGTGGGACAA CAGTACCTTC ATGATGCACT CGGGGAGTTC
    ATCAAAGCCT TGTATGAATC AGATGAGAAC TGTGAAGTGG ATCCCAGCAA GTGTTCATCT
    AGTGAACTGA TGGACCATCA GAGCAACCTG AAAATGTGCT GTGAGTTGGC TTTCTGCAAG
    ATCATCAACT CATACTGTGT CTTCCCGCGT GAGTTGAAAG AAGTGTTTGC ATCATGGAAG
    CAGCAGTGCC TGAACCGAGG CAAGCAAGAC ATCAGTGAGC GGCTCATCAG TGCCTCATTG
    TTTCTCCGTT TTCTTTGCCC AGCCATTATG TCTCCCAGTC TTTTCAACCT GATGCAGGAA
    TATCCTGATG ACCGCACATC TCGGACACTA ACTCTTATTG CCAAGGTCAT TCAGAACCTG
    GCCAACTTTG CCAAGTTTGG TAACAAAGAG GAGTACATGG CATTCATGAA TGATTTTTTA
    GAACATGAAT GGGGTGGAAT GAAGCGGTTC CTTTTGGAGA TCTCTAATCC TGACACCATC
    TCAAACACCC CAGGCTTCGA TGGTTACATT GATTTGGGCA GAGAGCTGTC AGTTTTGCAT
    TCCTTACTAT GGGAAGTAGT TTCCCAACTT GATAAGGGTG AAAATTCCTT TCTACAGGCA
    ACTGTGGCAA AACTGGGACC TCTCCCTCGT GTTCTTGCTG ACATTACCAA GTCTCTGACT
    AATCCTACGC CAATACAGCA GCAGCTAAGA CGTTTCGCTG AGCACAGCTC CAGTCCAAAC
    GTCAGCGGAA GCCTCTCCTC TGGGCTGCAG AGAATATGTG AAGACCCCAC TGACAGTGAT
    TTGCATAAAC TAAAGTCTCC AAGCCAGGAT AACACAGACA GCTATTTCAG AGGAAAGACG
    TTATTGTTGG TTCAGCAAGC CTCCTCCCAG AGCATGACGT ACTCTGAAAA GGATGAAAAG
    GAAAACAGCC TTCCTAACGG TCGGAGCATC TCCCTCATGG ACCTCCAGGA CACTCATGCT
    GCTCAGGCGG AGCATGCATC TGTCATGCTT GATGTGCCTA TGCGCCTTGC AGGAAGCCAG
    CTTTCTATAA CCCAGGTAGC TAGCATCAAA CAGCTGCGGG AAACCCAGAG CACTCCCCAG
    AGTGCACCTC AAGTGAGAAG ACCCCTGCAC CCAGCCTTGA ACCAGCCAGG AAGTCTCCAG
    CCCTTGTCAT TCCAGAATCC TGTCTATCAC CTCAACAACC CAGTTCCAGC AATGCCAAAG
    GCCTCTGCGG ATTCCAGTTT GGAGAACCTA AGCACTGCCA GTTCTAGAAG CCAAAGCAAT
    AGTGAAGACT TCAAACTCAG TGGACCCAGC AATAGCAGCA TGGAAGATTT CACCAAAAGA
    AGCTCCCACA GTGAAGACTT CTCCAGGCGC CACACTGTAC CAGACAGACA CATACCTCTT
    GCTCTACCAC GACAAAATAG TACGGGGCAG TCCCAAATCC GAAAACTGGA CCATTCTGGG
    TTAGGTGCCC GAGCCAAAGC ACCCCCATCT CTGCCACACA GTGCTTCCTT GCGTAGCACA
    GGGAGCATGT CAGTGGCGTC TGCAGCCCTA ATGGCTGAAC CTGTGCAGAA CGGGAGCCGG
    TCCAGGCAGC AGTCCTCTTC CTCCAGAGAG AGCCCTGTTC CCAAAGTGAG AGCAATCCAG
    AGACAACAGA CACAACAGGT TCAGTCACCT GTGGACTCCG CTACAATGTC CCCAGTAGAA
    AGGACAGCAG CCTGGGTTCT GAACAATGGA CAGTATGAAG AGGATGTGGA AGAAACTGAA
    CAAAATCAAG ATGAAGCCAA GCATGCTGAA AAGTATGAAC AAGAAATTAC CAAGCTGAAG
    GAGCGCCTAC GAGTGTCCAG CCGGCGGTTG GAGGAGTATG AGCGCCGGCT GCTGGTGCAG
    GAGCAGCAGA TGCAGAAGCT GCTGCTGGAG TACAAGGCCC GGCTGGAGGA TAGTGAGGAG
    CGGCTGCGCA GACAGCAGGA GGAGAAGGAC AGCCAGATGA AAAGCATCAT CAGCAGGCTA
    ATGGCTGTGG AAGAAGAATT AAAGAAAGAT CATGCTGAGA TGCAAGCAGT TATTGATGCA
    AAGCAGAAAA TAATTGATGC ACAGGTCATA AATGGAAATG AGATTATTCA AACAGGAAAA
    GCGGATCGTG TCCCTGGATT CAGCCAACAC GAGACTGATG AGTGCGCTGA CACAAGTGAA
    GGAGCGGTAC AGCATGCAGG TCCGCAATGG CATCTCTCCC ACCAACCCCA CCAAGCTTTC
    CATCACGGAG AATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu28685
    Clone ID Related Accession (Same CDS sequence) XM_006496754.3
    Accession Version XM_006496754.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3486bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product ras GTPase-activating protein nGAP isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006496754.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTTATCTG AGAAGCTGGA TTCTTATCTG TCTGTCAGTG AAGTGGAGAT GGGAAGCAGT 
    TGTGATAAAG AGGTGCCAGC AGAAAGGTCC CCCCGCAGAC GGAGCATCTC AGGGACCAGC
    ACATCAGAGA AACCCAACTC CATGGACACT GCGAATACCT CTCCTTTTAA AGTACCAGGA
    TTCTTCAGCA AGCGCCTGAA AGGTTCCATC AAGAGGACCA AAAGCCAGTC GAAGCTTGAC
    AGAAACACAA GCTTTCGGCT TCCATCCCTT CGCAATGCAG ATGACAGGTC TCGAGGTCTG
    CCTAAACTGA AAGAGTCGCG TTCCCATGAG TCCCTGCTGA GCCCATGCAG TGCAGTGGAG
    TGTCTGGATC TAGGTAGAGG AGAGCCAGTC TCAGTGAAGC CACTGCACAG TAGCATCCTC
    GGACAGGACT TCTGCTTCGA GGTCACCTAC TTAAGTGGAA GTAAATGCTT TAGCTGTAGC
    TCTGCCTCCG AGAGAGATAA GTGGATGGAA AACCTTCGAA GGACCGTTCA GCCAAATAAG
    GACAATTGTA GACGAGCTGA AAATGTTCTC CGTTTATGGA TCATTGAAGC CAAGGACCTT
    GCCCCTAAAA AAAAATATTT CTGCGAACTG TGCCTTGATG ATACCCTCTT TGCTCGTACA
    ACCAGCAAGA CCAAAGCAGA CAATATTTTT TGGGGTGAAC ATTTTGAATT CTATAGCCTT
    CCACCTCTTC ATAGCATCAC GGTTCATATT TATAAGGATG TGGAAAAAAA GAAAAAAAAG
    GACAAGAATA ATTATGTAGG GCTAGTCAAT ATCCCCACTG CCAGTGTGAC TGGTCGCCAG
    TTTGTAGAAA AGTGGTACCC AGTGAGTACA CCCACACCCA ACAAAGGAAA GACAGGAGGA
    CCTTCTATTC GGATTAAGTC ACGTTTCCAA ACTATCACCA TTCTGCCTAT GGAGCAATAC
    AAAGAGTTTG CAGAATTCAT CACCAGCAAC TACACCATGC TGTGTTCTGT TCTTGAGCCA
    GTAATTAGTG TGAGGAATAA AGAGGAGTTG GCTTGTGCCT TAGTACACAT TCTTCAAAGT
    ACTGGCAGAG CCAAGGATTT TCTGACTGAC TTGGTGATGT CAGAGGTAGA TCGTTGTGGA
    GAACATGATG TCTTGATCTT CAGAGAAAAC ACCATTGCCA CTAAATCCAT TGAGGAATAC
    CTCAAGTTGG TGGGACAACA GTACCTTCAT GATGCACTCG GGGAGTTCAT CAAAGCCTTG
    TATGAATCAG ATGAGAACTG TGAAGTGGAT CCCAGCAAGT GTTCATCTAG TGAACTGATG
    GACCATCAGA GCAACCTGAA AATGTGCTGT GAGTTGGCTT TCTGCAAGAT CATCAACTCA
    TACTGTGTCT TCCCGCGTGA GTTGAAAGAA GTGTTTGCAT CATGGAAGCA GCAGTGCCTG
    AACCGAGGCA AGCAAGACAT CAGTGAGCGG CTCATCAGTG CCTCATTGTT TCTCCGTTTT
    CTTTGCCCAG CCATTATGTC TCCCAGTCTT TTCAACCTGA TGCAGGAATA TCCTGATGAC
    CGCACATCTC GGACACTAAC TCTTATTGCC AAGGTCATTC AGAACCTGGC CAACTTTGCC
    AAGTTTGGTA ACAAAGAGGA GTACATGGCA TTCATGAATG ATTTTTTAGA ACATGAATGG
    GGTGGAATGA AGCGGTTCCT TTTGGAGATC TCTAATCCTG ACACCATCTC AAACACCCCA
    GGCTTCGATG GTTACATTGA TTTGGGCAGA GAGCTGTCAG TTTTGCATTC CTTACTATGG
    GAAGTAGTTT CCCAACTTGA TAAGGGTGAA AATTCCTTTC TACAGGCAAC TGTGGCAAAA
    CTGGGACCTC TCCCTCGTGT TCTTGCTGAC ATTACCAAGT CTCTGACTAA TCCTACGCCA
    ATACAGCAGC AGCTAAGACG TTTCGCTGAG CACAGCTCCA GTCCAAACGT CAGCGGAAGC
    CTCTCCTCTG GGCTGCAGAG AATATGTGAA GACCCCACTG ACAGTGATTT GCATAAACTA
    AAGTCTCCAA GCCAGGATAA CACAGACAGC TATTTCAGAG GAAAGACGTT ATTGTTGGTT
    CAGCAAGCCT CCTCCCAGAG CATGACGTAC TCTGAAAAGG ATGAAAAGGA AAACAGCCTT
    CCTAACGGTC GGAGCATCTC CCTCATGGAC CTCCAGGACA CTCATGCTGC TCAGGCGGAG
    CATGCATCTG TCATGCTTGA TGTGCCTATG CGCCTTGCAG GAAGCCAGCT TTCTATAACC
    CAGGTAGCTA GCATCAAACA GCTGCGGGAA ACCCAGAGCA CTCCCCAGAG TGCACCTCAA
    GTGAGAAGAC CCCTGCACCC AGCCTTGAAC CAGCCAGGAA GTCTCCAGCC CTTGTCATTC
    CAGAATCCTG TCTATCACCT CAACAACCCA GTTCCAGCAA TGCCAAAGGC CTCTGCGGAT
    TCCAGTTTGG AGAACCTAAG CACTGCCAGT TCTAGAAGCC AAAGCAATAG TGAAGACTTC
    AAACTCAGTG GACCCAGCAA TAGCAGCATG GAAGATTTCA CCAAAAGAAG CTCCCACAGT
    GAAGACTTCT CCAGGCGCCA CACTGTACCA GACAGACACA TACCTCTTGC TCTACCACGA
    CAAAATAGTA CGGGGCAGTC CCAAATCCGA AAACTGGACC ATTCTGGGTT AGGTGCCCGA
    GCCAAAGCAC CCCCATCTCT GCCACACAGT GCTTCCTTGC GTAGCACAGG GAGCATGTCA
    GTGGCGTCTG CAGCCCTAAT GGCTGAACCT GTGCAGAACG GGAGCCGGTC CAGGCAGCAG
    TCCTCTTCCT CCAGAGAGAG CCCTGTTCCC AAAGTGAGAG CAATCCAGAG ACAACAGACA
    CAACAGGTTC AGTCACCTGT GGACTCCGCT ACAATGTCCC CAGTAGAAAG GACAGCAGCC
    TGGGTTCTGA ACAATGGACA GTATGAAGAG GATGTGGAAG AAACTGAACA AAATCAAGAT
    GAAGCCAAGC ATGCTGAAAA GTATGAACAA GAAATTACCA AGCTGAAGGA GCGCCTACGA
    GTGTCCAGCC GGCGGTTGGA GGAGTATGAG CGCCGGCTGC TGGTGCAGGA GCAGCAGATG
    CAGAAGCTGC TGCTGGAGTA CAAGGCCCGG CTGGAGGATA GTGAGGAGCG GCTGCGCAGA
    CAGCAGGAGG AGAAGGACAG CCAGATGAAA AGCATCATCA GCAGGCTAAT GGCTGTGGAA
    GAAGAATTAA AGAAAGATCA TGCTGAGATG CAAGCAGTTA TTGATGCAAA GCAGAAAATA
    ATTGATGCAC AGGTCATAAA TGGAAATGAG ATTATTCAAA CAGGAAAAGC GGATCGTGTC
    CCTGGATTCA GCCAACACGA GACTGATGAG TGCGCTGACA CAAGTGAAGG AGCGGTACAG
    CATGCAGGTC CGCAATGGCA TCTCTCCCAC CAACCCCACC AAGCTTTCCA TCACGGAGAA
    TGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006496817.1
    CDS363..3848
    Translation

    Target ORF information:

    RefSeq Version XM_006496754.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus RAS protein activator like 2 (Rasal2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006496754.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTTATCTG AGAAGCTGGA TTCTTATCTG TCTGTCAGTG AAGTGGAGAT GGGAAGCAGT 
    TGTGATAAAG AGGTGCCAGC AGAAAGGTCC CCCCGCAGAC GGAGCATCTC AGGGACCAGC
    ACATCAGAGA AACCCAACTC CATGGACACT GCGAATACCT CTCCTTTTAA AGTACCAGGA
    TTCTTCAGCA AGCGCCTGAA AGGTTCCATC AAGAGGACCA AAAGCCAGTC GAAGCTTGAC
    AGAAACACAA GCTTTCGGCT TCCATCCCTT CGCAATGCAG ATGACAGGTC TCGAGGTCTG
    CCTAAACTGA AAGAGTCGCG TTCCCATGAG TCCCTGCTGA GCCCATGCAG TGCAGTGGAG
    TGTCTGGATC TAGGTAGAGG AGAGCCAGTC TCAGTGAAGC CACTGCACAG TAGCATCCTC
    GGACAGGACT TCTGCTTCGA GGTCACCTAC TTAAGTGGAA GTAAATGCTT TAGCTGTAGC
    TCTGCCTCCG AGAGAGATAA GTGGATGGAA AACCTTCGAA GGACCGTTCA GCCAAATAAG
    GACAATTGTA GACGAGCTGA AAATGTTCTC CGTTTATGGA TCATTGAAGC CAAGGACCTT
    GCCCCTAAAA AAAAATATTT CTGCGAACTG TGCCTTGATG ATACCCTCTT TGCTCGTACA
    ACCAGCAAGA CCAAAGCAGA CAATATTTTT TGGGGTGAAC ATTTTGAATT CTATAGCCTT
    CCACCTCTTC ATAGCATCAC GGTTCATATT TATAAGGATG TGGAAAAAAA GAAAAAAAAG
    GACAAGAATA ATTATGTAGG GCTAGTCAAT ATCCCCACTG CCAGTGTGAC TGGTCGCCAG
    TTTGTAGAAA AGTGGTACCC AGTGAGTACA CCCACACCCA ACAAAGGAAA GACAGGAGGA
    CCTTCTATTC GGATTAAGTC ACGTTTCCAA ACTATCACCA TTCTGCCTAT GGAGCAATAC
    AAAGAGTTTG CAGAATTCAT CACCAGCAAC TACACCATGC TGTGTTCTGT TCTTGAGCCA
    GTAATTAGTG TGAGGAATAA AGAGGAGTTG GCTTGTGCCT TAGTACACAT TCTTCAAAGT
    ACTGGCAGAG CCAAGGATTT TCTGACTGAC TTGGTGATGT CAGAGGTAGA TCGTTGTGGA
    GAACATGATG TCTTGATCTT CAGAGAAAAC ACCATTGCCA CTAAATCCAT TGAGGAATAC
    CTCAAGTTGG TGGGACAACA GTACCTTCAT GATGCACTCG GGGAGTTCAT CAAAGCCTTG
    TATGAATCAG ATGAGAACTG TGAAGTGGAT CCCAGCAAGT GTTCATCTAG TGAACTGATG
    GACCATCAGA GCAACCTGAA AATGTGCTGT GAGTTGGCTT TCTGCAAGAT CATCAACTCA
    TACTGTGTCT TCCCGCGTGA GTTGAAAGAA GTGTTTGCAT CATGGAAGCA GCAGTGCCTG
    AACCGAGGCA AGCAAGACAT CAGTGAGCGG CTCATCAGTG CCTCATTGTT TCTCCGTTTT
    CTTTGCCCAG CCATTATGTC TCCCAGTCTT TTCAACCTGA TGCAGGAATA TCCTGATGAC
    CGCACATCTC GGACACTAAC TCTTATTGCC AAGGTCATTC AGAACCTGGC CAACTTTGCC
    AAGTTTGGTA ACAAAGAGGA GTACATGGCA TTCATGAATG ATTTTTTAGA ACATGAATGG
    GGTGGAATGA AGCGGTTCCT TTTGGAGATC TCTAATCCTG ACACCATCTC AAACACCCCA
    GGCTTCGATG GTTACATTGA TTTGGGCAGA GAGCTGTCAG TTTTGCATTC CTTACTATGG
    GAAGTAGTTT CCCAACTTGA TAAGGGTGAA AATTCCTTTC TACAGGCAAC TGTGGCAAAA
    CTGGGACCTC TCCCTCGTGT TCTTGCTGAC ATTACCAAGT CTCTGACTAA TCCTACGCCA
    ATACAGCAGC AGCTAAGACG TTTCGCTGAG CACAGCTCCA GTCCAAACGT CAGCGGAAGC
    CTCTCCTCTG GGCTGCAGAG AATATGTGAA GACCCCACTG ACAGTGATTT GCATAAACTA
    AAGTCTCCAA GCCAGGATAA CACAGACAGC TATTTCAGAG GAAAGACGTT ATTGTTGGTT
    CAGCAAGCCT CCTCCCAGAG CATGACGTAC TCTGAAAAGG ATGAAAAGGA AAACAGCCTT
    CCTAACGGTC GGAGCATCTC CCTCATGGAC CTCCAGGACA CTCATGCTGC TCAGGCGGAG
    CATGCATCTG TCATGCTTGA TGTGCCTATG CGCCTTGCAG GAAGCCAGCT TTCTATAACC
    CAGGTAGCTA GCATCAAACA GCTGCGGGAA ACCCAGAGCA CTCCCCAGAG TGCACCTCAA
    GTGAGAAGAC CCCTGCACCC AGCCTTGAAC CAGCCAGGAA GTCTCCAGCC CTTGTCATTC
    CAGAATCCTG TCTATCACCT CAACAACCCA GTTCCAGCAA TGCCAAAGGC CTCTGCGGAT
    TCCAGTTTGG AGAACCTAAG CACTGCCAGT TCTAGAAGCC AAAGCAATAG TGAAGACTTC
    AAACTCAGTG GACCCAGCAA TAGCAGCATG GAAGATTTCA CCAAAAGAAG CTCCCACAGT
    GAAGACTTCT CCAGGCGCCA CACTGTACCA GACAGACACA TACCTCTTGC TCTACCACGA
    CAAAATAGTA CGGGGCAGTC CCAAATCCGA AAACTGGACC ATTCTGGGTT AGGTGCCCGA
    GCCAAAGCAC CCCCATCTCT GCCACACAGT GCTTCCTTGC GTAGCACAGG GAGCATGTCA
    GTGGCGTCTG CAGCCCTAAT GGCTGAACCT GTGCAGAACG GGAGCCGGTC CAGGCAGCAG
    TCCTCTTCCT CCAGAGAGAG CCCTGTTCCC AAAGTGAGAG CAATCCAGAG ACAACAGACA
    CAACAGGTTC AGTCACCTGT GGACTCCGCT ACAATGTCCC CAGTAGAAAG GACAGCAGCC
    TGGGTTCTGA ACAATGGACA GTATGAAGAG GATGTGGAAG AAACTGAACA AAATCAAGAT
    GAAGCCAAGC ATGCTGAAAA GTATGAACAA GAAATTACCA AGCTGAAGGA GCGCCTACGA
    GTGTCCAGCC GGCGGTTGGA GGAGTATGAG CGCCGGCTGC TGGTGCAGGA GCAGCAGATG
    CAGAAGCTGC TGCTGGAGTA CAAGGCCCGG CTGGAGGATA GTGAGGAGCG GCTGCGCAGA
    CAGCAGGAGG AGAAGGACAG CCAGATGAAA AGCATCATCA GCAGGCTAAT GGCTGTGGAA
    GAAGAATTAA AGAAAGATCA TGCTGAGATG CAAGCAGTTA TTGATGCAAA GCAGAAAATA
    ATTGATGCAC AGGTCATAAA TGGAAATGAG ATTATTCAAA CAGGAAAAGC GGATCGTGTC
    CCTGGATTCA GCCAACACGA GACTGATGAG TGCGCTGACA CAAGTGAAGG AGCGGTACAG
    CATGCAGGTC CGCAATGGCA TCTCTCCCAC CAACCCCACC AAGCTTTCCA TCACGGAGAA
    TGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu28686
    Clone ID Related Accession (Same CDS sequence) XM_006496755.3 , XM_006496755.2
    Accession Version XM_006496755.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3429bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product ras GTPase-activating protein nGAP isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006496755.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCAGACCC CAGAGGTGCC AGCAGAAAGG TCCCCCCGCA GACGGAGCAT CTCAGGGACC 
    AGCACATCAG AGAAACCCAA CTCCATGGAC ACTGCGAATA CCTCTCCTTT TAAAGTACCA
    GGATTCTTCA GCAAGCGCCT GAAAGGTTCC ATCAAGAGGA CCAAAAGCCA GTCGAAGCTT
    GACAGAAACA CAAGCTTTCG GCTTCCATCC CTTCGCAATG CAGATGACAG GTCTCGAGGT
    CTGCCTAAAC TGAAAGAGTC GCGTTCCCAT GAGTCCCTGC TGAGCCCATG CAGTGCAGTG
    GAGTGTCTGG ATCTAGGTAG AGGAGAGCCA GTCTCAGTGA AGCCACTGCA CAGTAGCATC
    CTCGGACAGG ACTTCTGCTT CGAGGTCACC TACTTAAGTG GAAGTAAATG CTTTAGCTGT
    AGCTCTGCCT CCGAGAGAGA TAAGTGGATG GAAAACCTTC GAAGGACCGT TCAGCCAAAT
    AAGGACAATT GTAGACGAGC TGAAAATGTT CTCCGTTTAT GGATCATTGA AGCCAAGGAC
    CTTGCCCCTA AAAAAAAATA TTTCTGCGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT
    ACAACCAGCA AGACCAAAGC AGACAATATT TTTTGGGGTG AACATTTTGA ATTCTATAGC
    CTTCCACCTC TTCATAGCAT CACGGTTCAT ATTTATAAGG ATGTGGAAAA AAAGAAAAAA
    AAGGACAAGA ATAATTATGT AGGGCTAGTC AATATCCCCA CTGCCAGTGT GACTGGTCGC
    CAGTTTGTAG AAAAGTGGTA CCCAGTGAGT ACACCCACAC CCAACAAAGG AAAGACAGGA
    GGACCTTCTA TTCGGATTAA GTCACGTTTC CAAACTATCA CCATTCTGCC TATGGAGCAA
    TACAAAGAGT TTGCAGAATT CATCACCAGC AACTACACCA TGCTGTGTTC TGTTCTTGAG
    CCAGTAATTA GTGTGAGGAA TAAAGAGGAG TTGGCTTGTG CCTTAGTACA CATTCTTCAA
    AGTACTGGCA GAGCCAAGGA TTTTCTGACT GACTTGGTGA TGTCAGAGGT AGATCGTTGT
    GGAGAACATG ATGTCTTGAT CTTCAGAGAA AACACCATTG CCACTAAATC CATTGAGGAA
    TACCTCAAGT TGGTGGGACA ACAGTACCTT CATGATGCAC TCGGGGAGTT CATCAAAGCC
    TTGTATGAAT CAGATGAGAA CTGTGAAGTG GATCCCAGCA AGTGTTCATC TAGTGAACTG
    ATGGACCATC AGAGCAACCT GAAAATGTGC TGTGAGTTGG CTTTCTGCAA GATCATCAAC
    TCATACTGTG TCTTCCCGCG TGAGTTGAAA GAAGTGTTTG CATCATGGAA GCAGCAGTGC
    CTGAACCGAG GCAAGCAAGA CATCAGTGAG CGGCTCATCA GTGCCTCATT GTTTCTCCGT
    TTTCTTTGCC CAGCCATTAT GTCTCCCAGT CTTTTCAACC TGATGCAGGA ATATCCTGAT
    GACCGCACAT CTCGGACACT AACTCTTATT GCCAAGGTCA TTCAGAACCT GGCCAACTTT
    GCCAAGTTTG GTAACAAAGA GGAGTACATG GCATTCATGA ATGATTTTTT AGAACATGAA
    TGGGGTGGAA TGAAGCGGTT CCTTTTGGAG ATCTCTAATC CTGACACCAT CTCAAACACC
    CCAGGCTTCG ATGGTTACAT TGATTTGGGC AGAGAGCTGT CAGTTTTGCA TTCCTTACTA
    TGGGAAGTAG TTTCCCAACT TGATAAGGGT GAAAATTCCT TTCTACAGGC AACTGTGGCA
    AAACTGGGAC CTCTCCCTCG TGTTCTTGCT GACATTACCA AGTCTCTGAC TAATCCTACG
    CCAATACAGC AGCAGCTAAG ACGTTTCGCT GAGCACAGCT CCAGTCCAAA CGTCAGCGGA
    AGCCTCTCCT CTGGGCTGCA GAGAATATGT GAAGACCCCA CTGACAGTGA TTTGCATAAA
    CTAAAGTCTC CAAGCCAGGA TAACACAGAC AGCTATTTCA GAGGAAAGAC GTTATTGTTG
    GTTCAGCAAG CCTCCTCCCA GAGCATGACG TACTCTGAAA AGGATGAAAA GGAAAACAGC
    CTTCCTAACG GTCGGAGCAT CTCCCTCATG GACCTCCAGG ACACTCATGC TGCTCAGGCG
    GAGCATGCAT CTGTCATGCT TGATGTGCCT ATGCGCCTTG CAGGAAGCCA GCTTTCTATA
    ACCCAGGTAG CTAGCATCAA ACAGCTGCGG GAAACCCAGA GCACTCCCCA GAGTGCACCT
    CAAGTGAGAA GACCCCTGCA CCCAGCCTTG AACCAGCCAG GAAGTCTCCA GCCCTTGTCA
    TTCCAGAATC CTGTCTATCA CCTCAACAAC CCAGTTCCAG CAATGCCAAA GGCCTCTGCG
    GATTCCAGTT TGGAGAACCT AAGCACTGCC AGTTCTAGAA GCCAAAGCAA TAGTGAAGAC
    TTCAAACTCA GTGGACCCAG CAATAGCAGC ATGGAAGATT TCACCAAAAG AAGCTCCCAC
    AGTGAAGACT TCTCCAGGCG CCACACTGTA CCAGACAGAC ACATACCTCT TGCTCTACCA
    CGACAAAATA GTACGGGGCA GTCCCAAATC CGAAAACTGG ACCATTCTGG GTTAGGTGCC
    CGAGCCAAAG CACCCCCATC TCTGCCACAC AGTGCTTCCT TGCGTAGCAC AGGGAGCATG
    TCAGTGGCGT CTGCAGCCCT AATGGCTGAA CCTGTGCAGA ACGGGAGCCG GTCCAGGCAG
    CAGTCCTCTT CCTCCAGAGA GAGCCCTGTT CCCAAAGTGA GAGCAATCCA GAGACAACAG
    ACACAACAGG TTCAGTCACC TGTGGACTCC GCTACAATGT CCCCAGTAGA AAGGACAGCA
    GCCTGGGTTC TGAACAATGG ACAGTATGAA GAGGATGTGG AAGAAACTGA ACAAAATCAA
    GATGAAGCCA AGCATGCTGA AAAGTATGAA CAAGAAATTA CCAAGCTGAA GGAGCGCCTA
    CGAGTGTCCA GCCGGCGGTT GGAGGAGTAT GAGCGCCGGC TGCTGGTGCA GGAGCAGCAG
    ATGCAGAAGC TGCTGCTGGA GTACAAGGCC CGGCTGGAGG ATAGTGAGGA GCGGCTGCGC
    AGACAGCAGG AGGAGAAGGA CAGCCAGATG AAAAGCATCA TCAGCAGGCT AATGGCTGTG
    GAAGAAGAAT TAAAGAAAGA TCATGCTGAG ATGCAAGCAG TTATTGATGC AAAGCAGAAA
    ATAATTGATG CACAGGTCAT AAATGGAAAT GAGATTATTC AAACAGGAAA AGCGGATCGT
    GTCCCTGGAT TCAGCCAACA CGAGACTGAT GAGTGCGCTG ACACAAGTGA AGGAGCGGTA
    CAGCATGCAG GTCCGCAATG GCATCTCTCC CACCAACCCC ACCAAGCTTT CCATCACGGA
    GAATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006496818.1
    CDS123..3551
    Translation

    Target ORF information:

    RefSeq Version XM_006496755.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus RAS protein activator like 2 (Rasal2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006496755.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCAGACCC CAGAGGTGCC AGCAGAAAGG TCCCCCCGCA GACGGAGCAT CTCAGGGACC 
    AGCACATCAG AGAAACCCAA CTCCATGGAC ACTGCGAATA CCTCTCCTTT TAAAGTACCA
    GGATTCTTCA GCAAGCGCCT GAAAGGTTCC ATCAAGAGGA CCAAAAGCCA GTCGAAGCTT
    GACAGAAACA CAAGCTTTCG GCTTCCATCC CTTCGCAATG CAGATGACAG GTCTCGAGGT
    CTGCCTAAAC TGAAAGAGTC GCGTTCCCAT GAGTCCCTGC TGAGCCCATG CAGTGCAGTG
    GAGTGTCTGG ATCTAGGTAG AGGAGAGCCA GTCTCAGTGA AGCCACTGCA CAGTAGCATC
    CTCGGACAGG ACTTCTGCTT CGAGGTCACC TACTTAAGTG GAAGTAAATG CTTTAGCTGT
    AGCTCTGCCT CCGAGAGAGA TAAGTGGATG GAAAACCTTC GAAGGACCGT TCAGCCAAAT
    AAGGACAATT GTAGACGAGC TGAAAATGTT CTCCGTTTAT GGATCATTGA AGCCAAGGAC
    CTTGCCCCTA AAAAAAAATA TTTCTGCGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT
    ACAACCAGCA AGACCAAAGC AGACAATATT TTTTGGGGTG AACATTTTGA ATTCTATAGC
    CTTCCACCTC TTCATAGCAT CACGGTTCAT ATTTATAAGG ATGTGGAAAA AAAGAAAAAA
    AAGGACAAGA ATAATTATGT AGGGCTAGTC AATATCCCCA CTGCCAGTGT GACTGGTCGC
    CAGTTTGTAG AAAAGTGGTA CCCAGTGAGT ACACCCACAC CCAACAAAGG AAAGACAGGA
    GGACCTTCTA TTCGGATTAA GTCACGTTTC CAAACTATCA CCATTCTGCC TATGGAGCAA
    TACAAAGAGT TTGCAGAATT CATCACCAGC AACTACACCA TGCTGTGTTC TGTTCTTGAG
    CCAGTAATTA GTGTGAGGAA TAAAGAGGAG TTGGCTTGTG CCTTAGTACA CATTCTTCAA
    AGTACTGGCA GAGCCAAGGA TTTTCTGACT GACTTGGTGA TGTCAGAGGT AGATCGTTGT
    GGAGAACATG ATGTCTTGAT CTTCAGAGAA AACACCATTG CCACTAAATC CATTGAGGAA
    TACCTCAAGT TGGTGGGACA ACAGTACCTT CATGATGCAC TCGGGGAGTT CATCAAAGCC
    TTGTATGAAT CAGATGAGAA CTGTGAAGTG GATCCCAGCA AGTGTTCATC TAGTGAACTG
    ATGGACCATC AGAGCAACCT GAAAATGTGC TGTGAGTTGG CTTTCTGCAA GATCATCAAC
    TCATACTGTG TCTTCCCGCG TGAGTTGAAA GAAGTGTTTG CATCATGGAA GCAGCAGTGC
    CTGAACCGAG GCAAGCAAGA CATCAGTGAG CGGCTCATCA GTGCCTCATT GTTTCTCCGT
    TTTCTTTGCC CAGCCATTAT GTCTCCCAGT CTTTTCAACC TGATGCAGGA ATATCCTGAT
    GACCGCACAT CTCGGACACT AACTCTTATT GCCAAGGTCA TTCAGAACCT GGCCAACTTT
    GCCAAGTTTG GTAACAAAGA GGAGTACATG GCATTCATGA ATGATTTTTT AGAACATGAA
    TGGGGTGGAA TGAAGCGGTT CCTTTTGGAG ATCTCTAATC CTGACACCAT CTCAAACACC
    CCAGGCTTCG ATGGTTACAT TGATTTGGGC AGAGAGCTGT CAGTTTTGCA TTCCTTACTA
    TGGGAAGTAG TTTCCCAACT TGATAAGGGT GAAAATTCCT TTCTACAGGC AACTGTGGCA
    AAACTGGGAC CTCTCCCTCG TGTTCTTGCT GACATTACCA AGTCTCTGAC TAATCCTACG
    CCAATACAGC AGCAGCTAAG ACGTTTCGCT GAGCACAGCT CCAGTCCAAA CGTCAGCGGA
    AGCCTCTCCT CTGGGCTGCA GAGAATATGT GAAGACCCCA CTGACAGTGA TTTGCATAAA
    CTAAAGTCTC CAAGCCAGGA TAACACAGAC AGCTATTTCA GAGGAAAGAC GTTATTGTTG
    GTTCAGCAAG CCTCCTCCCA GAGCATGACG TACTCTGAAA AGGATGAAAA GGAAAACAGC
    CTTCCTAACG GTCGGAGCAT CTCCCTCATG GACCTCCAGG ACACTCATGC TGCTCAGGCG
    GAGCATGCAT CTGTCATGCT TGATGTGCCT ATGCGCCTTG CAGGAAGCCA GCTTTCTATA
    ACCCAGGTAG CTAGCATCAA ACAGCTGCGG GAAACCCAGA GCACTCCCCA GAGTGCACCT
    CAAGTGAGAA GACCCCTGCA CCCAGCCTTG AACCAGCCAG GAAGTCTCCA GCCCTTGTCA
    TTCCAGAATC CTGTCTATCA CCTCAACAAC CCAGTTCCAG CAATGCCAAA GGCCTCTGCG
    GATTCCAGTT TGGAGAACCT AAGCACTGCC AGTTCTAGAA GCCAAAGCAA TAGTGAAGAC
    TTCAAACTCA GTGGACCCAG CAATAGCAGC ATGGAAGATT TCACCAAAAG AAGCTCCCAC
    AGTGAAGACT TCTCCAGGCG CCACACTGTA CCAGACAGAC ACATACCTCT TGCTCTACCA
    CGACAAAATA GTACGGGGCA GTCCCAAATC CGAAAACTGG ACCATTCTGG GTTAGGTGCC
    CGAGCCAAAG CACCCCCATC TCTGCCACAC AGTGCTTCCT TGCGTAGCAC AGGGAGCATG
    TCAGTGGCGT CTGCAGCCCT AATGGCTGAA CCTGTGCAGA ACGGGAGCCG GTCCAGGCAG
    CAGTCCTCTT CCTCCAGAGA GAGCCCTGTT CCCAAAGTGA GAGCAATCCA GAGACAACAG
    ACACAACAGG TTCAGTCACC TGTGGACTCC GCTACAATGT CCCCAGTAGA AAGGACAGCA
    GCCTGGGTTC TGAACAATGG ACAGTATGAA GAGGATGTGG AAGAAACTGA ACAAAATCAA
    GATGAAGCCA AGCATGCTGA AAAGTATGAA CAAGAAATTA CCAAGCTGAA GGAGCGCCTA
    CGAGTGTCCA GCCGGCGGTT GGAGGAGTAT GAGCGCCGGC TGCTGGTGCA GGAGCAGCAG
    ATGCAGAAGC TGCTGCTGGA GTACAAGGCC CGGCTGGAGG ATAGTGAGGA GCGGCTGCGC
    AGACAGCAGG AGGAGAAGGA CAGCCAGATG AAAAGCATCA TCAGCAGGCT AATGGCTGTG
    GAAGAAGAAT TAAAGAAAGA TCATGCTGAG ATGCAAGCAG TTATTGATGC AAAGCAGAAA
    ATAATTGATG CACAGGTCAT AAATGGAAAT GAGATTATTC AAACAGGAAA AGCGGATCGT
    GTCCCTGGAT TCAGCCAACA CGAGACTGAT GAGTGCGCTG ACACAAGTGA AGGAGCGGTA
    CAGCATGCAG GTCCGCAATG GCATCTCTCC CACCAACCCC ACCAAGCTTT CCATCACGGA
    GAATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu28687
    Clone ID Related Accession (Same CDS sequence) XM_006496756.3 , XM_006496756.4
    Accession Version XM_006496756.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3345bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product ras GTPase-activating protein nGAP isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006496756.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGACACTG CGAATACCTC TCCTTTTAAA GTACCAGGAT TCTTCAGCAA GCGCCTGAAA 
    GGTTCCATCA AGAGGACCAA AAGCCAGTCG AAGCTTGACA GAAACACAAG CTTTCGGCTT
    CCATCCCTTC GCAATGCAGA TGACAGGTCT CGAGGTCTGC CTAAACTGAA AGAGTCGCGT
    TCCCATGAGT CCCTGCTGAG CCCATGCAGT GCAGTGGAGT GTCTGGATCT AGGTAGAGGA
    GAGCCAGTCT CAGTGAAGCC ACTGCACAGT AGCATCCTCG GACAGGACTT CTGCTTCGAG
    GTCACCTACT TAAGTGGAAG TAAATGCTTT AGCTGTAGCT CTGCCTCCGA GAGAGATAAG
    TGGATGGAAA ACCTTCGAAG GACCGTTCAG CCAAATAAGG ACAATTGTAG ACGAGCTGAA
    AATGTTCTCC GTTTATGGAT CATTGAAGCC AAGGACCTTG CCCCTAAAAA AAAATATTTC
    TGCGAACTGT GCCTTGATGA TACCCTCTTT GCTCGTACAA CCAGCAAGAC CAAAGCAGAC
    AATATTTTTT GGGGTGAACA TTTTGAATTC TATAGCCTTC CACCTCTTCA TAGCATCACG
    GTTCATATTT ATAAGGATGT GGAAAAAAAG AAAAAAAAGG ACAAGAATAA TTATGTAGGG
    CTAGTCAATA TCCCCACTGC CAGTGTGACT GGTCGCCAGT TTGTAGAAAA GTGGTACCCA
    GTGAGTACAC CCACACCCAA CAAAGGAAAG ACAGGAGGAC CTTCTATTCG GATTAAGTCA
    CGTTTCCAAA CTATCACCAT TCTGCCTATG GAGCAATACA AAGAGTTTGC AGAATTCATC
    ACCAGCAACT ACACCATGCT GTGTTCTGTT CTTGAGCCAG TAATTAGTGT GAGGAATAAA
    GAGGAGTTGG CTTGTGCCTT AGTACACATT CTTCAAAGTA CTGGCAGAGC CAAGGATTTT
    CTGACTGACT TGGTGATGTC AGAGGTAGAT CGTTGTGGAG AACATGATGT CTTGATCTTC
    AGAGAAAACA CCATTGCCAC TAAATCCATT GAGGAATACC TCAAGTTGGT GGGACAACAG
    TACCTTCATG ATGCACTCGG GGAGTTCATC AAAGCCTTGT ATGAATCAGA TGAGAACTGT
    GAAGTGGATC CCAGCAAGTG TTCATCTAGT GAACTGATGG ACCATCAGAG CAACCTGAAA
    ATGTGCTGTG AGTTGGCTTT CTGCAAGATC ATCAACTCAT ACTGTGTCTT CCCGCGTGAG
    TTGAAAGAAG TGTTTGCATC ATGGAAGCAG CAGTGCCTGA ACCGAGGCAA GCAAGACATC
    AGTGAGCGGC TCATCAGTGC CTCATTGTTT CTCCGTTTTC TTTGCCCAGC CATTATGTCT
    CCCAGTCTTT TCAACCTGAT GCAGGAATAT CCTGATGACC GCACATCTCG GACACTAACT
    CTTATTGCCA AGGTCATTCA GAACCTGGCC AACTTTGCCA AGTTTGGTAA CAAAGAGGAG
    TACATGGCAT TCATGAATGA TTTTTTAGAA CATGAATGGG GTGGAATGAA GCGGTTCCTT
    TTGGAGATCT CTAATCCTGA CACCATCTCA AACACCCCAG GCTTCGATGG TTACATTGAT
    TTGGGCAGAG AGCTGTCAGT TTTGCATTCC TTACTATGGG AAGTAGTTTC CCAACTTGAT
    AAGGGTGAAA ATTCCTTTCT ACAGGCAACT GTGGCAAAAC TGGGACCTCT CCCTCGTGTT
    CTTGCTGACA TTACCAAGTC TCTGACTAAT CCTACGCCAA TACAGCAGCA GCTAAGACGT
    TTCGCTGAGC ACAGCTCCAG TCCAAACGTC AGCGGAAGCC TCTCCTCTGG GCTGCAGAGA
    ATATGTGAAG ACCCCACTGA CAGTGATTTG CATAAACTAA AGTCTCCAAG CCAGGATAAC
    ACAGACAGCT ATTTCAGAGG AAAGACGTTA TTGTTGGTTC AGCAAGCCTC CTCCCAGAGC
    ATGACGTACT CTGAAAAGGA TGAAAAGGAA AACAGCCTTC CTAACGGTCG GAGCATCTCC
    CTCATGGACC TCCAGGACAC TCATGCTGCT CAGGCGGAGC ATGCATCTGT CATGCTTGAT
    GTGCCTATGC GCCTTGCAGG AAGCCAGCTT TCTATAACCC AGGTAGCTAG CATCAAACAG
    CTGCGGGAAA CCCAGAGCAC TCCCCAGAGT GCACCTCAAG TGAGAAGACC CCTGCACCCA
    GCCTTGAACC AGCCAGGAAG TCTCCAGCCC TTGTCATTCC AGAATCCTGT CTATCACCTC
    AACAACCCAG TTCCAGCAAT GCCAAAGGCC TCTGCGGATT CCAGTTTGGA GAACCTAAGC
    ACTGCCAGTT CTAGAAGCCA AAGCAATAGT GAAGACTTCA AACTCAGTGG ACCCAGCAAT
    AGCAGCATGG AAGATTTCAC CAAAAGAAGC TCCCACAGTG AAGACTTCTC CAGGCGCCAC
    ACTGTACCAG ACAGACACAT ACCTCTTGCT CTACCACGAC AAAATAGTAC GGGGCAGTCC
    CAAATCCGAA AACTGGACCA TTCTGGGTTA GGTGCCCGAG CCAAAGCACC CCCATCTCTG
    CCACACAGTG CTTCCTTGCG TAGCACAGGG AGCATGTCAG TGGCGTCTGC AGCCCTAATG
    GCTGAACCTG TGCAGAACGG GAGCCGGTCC AGGCAGCAGT CCTCTTCCTC CAGAGAGAGC
    CCTGTTCCCA AAGTGAGAGC AATCCAGAGA CAACAGACAC AACAGGTTCA GTCACCTGTG
    GACTCCGCTA CAATGTCCCC AGTAGAAAGG ACAGCAGCCT GGGTTCTGAA CAATGGACAG
    TATGAAGAGG ATGTGGAAGA AACTGAACAA AATCAAGATG AAGCCAAGCA TGCTGAAAAG
    TATGAACAAG AAATTACCAA GCTGAAGGAG CGCCTACGAG TGTCCAGCCG GCGGTTGGAG
    GAGTATGAGC GCCGGCTGCT GGTGCAGGAG CAGCAGATGC AGAAGCTGCT GCTGGAGTAC
    AAGGCCCGGC TGGAGGATAG TGAGGAGCGG CTGCGCAGAC AGCAGGAGGA GAAGGACAGC
    CAGATGAAAA GCATCATCAG CAGGCTAATG GCTGTGGAAG AAGAATTAAA GAAAGATCAT
    GCTGAGATGC AAGCAGTTAT TGATGCAAAG CAGAAAATAA TTGATGCACA GGTCATAAAT
    GGAAATGAGA TTATTCAAAC AGGAAAAGCG GATCGTGTCC CTGGATTCAG CCAACACGAG
    ACTGATGAGT GCGCTGACAC AAGTGAAGGA GCGGTACAGC ATGCAGGTCC GCAATGGCAT
    CTCTCCCACC AACCCCACCA AGCTTTCCAT CACGGAGAAT GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006496819.1
    CDS1385..4729
    Translation

    Target ORF information:

    RefSeq Version XM_006496756.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus RAS protein activator like 2 (Rasal2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006496756.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGACACTG CGAATACCTC TCCTTTTAAA GTACCAGGAT TCTTCAGCAA GCGCCTGAAA 
    GGTTCCATCA AGAGGACCAA AAGCCAGTCG AAGCTTGACA GAAACACAAG CTTTCGGCTT
    CCATCCCTTC GCAATGCAGA TGACAGGTCT CGAGGTCTGC CTAAACTGAA AGAGTCGCGT
    TCCCATGAGT CCCTGCTGAG CCCATGCAGT GCAGTGGAGT GTCTGGATCT AGGTAGAGGA
    GAGCCAGTCT CAGTGAAGCC ACTGCACAGT AGCATCCTCG GACAGGACTT CTGCTTCGAG
    GTCACCTACT TAAGTGGAAG TAAATGCTTT AGCTGTAGCT CTGCCTCCGA GAGAGATAAG
    TGGATGGAAA ACCTTCGAAG GACCGTTCAG CCAAATAAGG ACAATTGTAG ACGAGCTGAA
    AATGTTCTCC GTTTATGGAT CATTGAAGCC AAGGACCTTG CCCCTAAAAA AAAATATTTC
    TGCGAACTGT GCCTTGATGA TACCCTCTTT GCTCGTACAA CCAGCAAGAC CAAAGCAGAC
    AATATTTTTT GGGGTGAACA TTTTGAATTC TATAGCCTTC CACCTCTTCA TAGCATCACG
    GTTCATATTT ATAAGGATGT GGAAAAAAAG AAAAAAAAGG ACAAGAATAA TTATGTAGGG
    CTAGTCAATA TCCCCACTGC CAGTGTGACT GGTCGCCAGT TTGTAGAAAA GTGGTACCCA
    GTGAGTACAC CCACACCCAA CAAAGGAAAG ACAGGAGGAC CTTCTATTCG GATTAAGTCA
    CGTTTCCAAA CTATCACCAT TCTGCCTATG GAGCAATACA AAGAGTTTGC AGAATTCATC
    ACCAGCAACT ACACCATGCT GTGTTCTGTT CTTGAGCCAG TAATTAGTGT GAGGAATAAA
    GAGGAGTTGG CTTGTGCCTT AGTACACATT CTTCAAAGTA CTGGCAGAGC CAAGGATTTT
    CTGACTGACT TGGTGATGTC AGAGGTAGAT CGTTGTGGAG AACATGATGT CTTGATCTTC
    AGAGAAAACA CCATTGCCAC TAAATCCATT GAGGAATACC TCAAGTTGGT GGGACAACAG
    TACCTTCATG ATGCACTCGG GGAGTTCATC AAAGCCTTGT ATGAATCAGA TGAGAACTGT
    GAAGTGGATC CCAGCAAGTG TTCATCTAGT GAACTGATGG ACCATCAGAG CAACCTGAAA
    ATGTGCTGTG AGTTGGCTTT CTGCAAGATC ATCAACTCAT ACTGTGTCTT CCCGCGTGAG
    TTGAAAGAAG TGTTTGCATC ATGGAAGCAG CAGTGCCTGA ACCGAGGCAA GCAAGACATC
    AGTGAGCGGC TCATCAGTGC CTCATTGTTT CTCCGTTTTC TTTGCCCAGC CATTATGTCT
    CCCAGTCTTT TCAACCTGAT GCAGGAATAT CCTGATGACC GCACATCTCG GACACTAACT
    CTTATTGCCA AGGTCATTCA GAACCTGGCC AACTTTGCCA AGTTTGGTAA CAAAGAGGAG
    TACATGGCAT TCATGAATGA TTTTTTAGAA CATGAATGGG GTGGAATGAA GCGGTTCCTT
    TTGGAGATCT CTAATCCTGA CACCATCTCA AACACCCCAG GCTTCGATGG TTACATTGAT
    TTGGGCAGAG AGCTGTCAGT TTTGCATTCC TTACTATGGG AAGTAGTTTC CCAACTTGAT
    AAGGGTGAAA ATTCCTTTCT ACAGGCAACT GTGGCAAAAC TGGGACCTCT CCCTCGTGTT
    CTTGCTGACA TTACCAAGTC TCTGACTAAT CCTACGCCAA TACAGCAGCA GCTAAGACGT
    TTCGCTGAGC ACAGCTCCAG TCCAAACGTC AGCGGAAGCC TCTCCTCTGG GCTGCAGAGA
    ATATGTGAAG ACCCCACTGA CAGTGATTTG CATAAACTAA AGTCTCCAAG CCAGGATAAC
    ACAGACAGCT ATTTCAGAGG AAAGACGTTA TTGTTGGTTC AGCAAGCCTC CTCCCAGAGC
    ATGACGTACT CTGAAAAGGA TGAAAAGGAA AACAGCCTTC CTAACGGTCG GAGCATCTCC
    CTCATGGACC TCCAGGACAC TCATGCTGCT CAGGCGGAGC ATGCATCTGT CATGCTTGAT
    GTGCCTATGC GCCTTGCAGG AAGCCAGCTT TCTATAACCC AGGTAGCTAG CATCAAACAG
    CTGCGGGAAA CCCAGAGCAC TCCCCAGAGT GCACCTCAAG TGAGAAGACC CCTGCACCCA
    GCCTTGAACC AGCCAGGAAG TCTCCAGCCC TTGTCATTCC AGAATCCTGT CTATCACCTC
    AACAACCCAG TTCCAGCAAT GCCAAAGGCC TCTGCGGATT CCAGTTTGGA GAACCTAAGC
    ACTGCCAGTT CTAGAAGCCA AAGCAATAGT GAAGACTTCA AACTCAGTGG ACCCAGCAAT
    AGCAGCATGG AAGATTTCAC CAAAAGAAGC TCCCACAGTG AAGACTTCTC CAGGCGCCAC
    ACTGTACCAG ACAGACACAT ACCTCTTGCT CTACCACGAC AAAATAGTAC GGGGCAGTCC
    CAAATCCGAA AACTGGACCA TTCTGGGTTA GGTGCCCGAG CCAAAGCACC CCCATCTCTG
    CCACACAGTG CTTCCTTGCG TAGCACAGGG AGCATGTCAG TGGCGTCTGC AGCCCTAATG
    GCTGAACCTG TGCAGAACGG GAGCCGGTCC AGGCAGCAGT CCTCTTCCTC CAGAGAGAGC
    CCTGTTCCCA AAGTGAGAGC AATCCAGAGA CAACAGACAC AACAGGTTCA GTCACCTGTG
    GACTCCGCTA CAATGTCCCC AGTAGAAAGG ACAGCAGCCT GGGTTCTGAA CAATGGACAG
    TATGAAGAGG ATGTGGAAGA AACTGAACAA AATCAAGATG AAGCCAAGCA TGCTGAAAAG
    TATGAACAAG AAATTACCAA GCTGAAGGAG CGCCTACGAG TGTCCAGCCG GCGGTTGGAG
    GAGTATGAGC GCCGGCTGCT GGTGCAGGAG CAGCAGATGC AGAAGCTGCT GCTGGAGTAC
    AAGGCCCGGC TGGAGGATAG TGAGGAGCGG CTGCGCAGAC AGCAGGAGGA GAAGGACAGC
    CAGATGAAAA GCATCATCAG CAGGCTAATG GCTGTGGAAG AAGAATTAAA GAAAGATCAT
    GCTGAGATGC AAGCAGTTAT TGATGCAAAG CAGAAAATAA TTGATGCACA GGTCATAAAT
    GGAAATGAGA TTATTCAAAC AGGAAAAGCG GATCGTGTCC CTGGATTCAG CCAACACGAG
    ACTGATGAGT GCGCTGACAC AAGTGAAGGA GCGGTACAGC ATGCAGGTCC GCAATGGCAT
    CTCTCCCACC AACCCCACCA AGCTTTCCAT CACGGAGAAT GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.