SPTAN1 cDNA ORF clone, Gallus gallus(chicken)

The following SPTAN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPTAN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa43308 XM_015279282.1
Latest version!
Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OGa43309 XM_015279283.1
Latest version!
Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OGa43310 XM_015279284.2
Latest version!
Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OGa43311 XM_015279285.2
Latest version!
Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OGa43312 XM_015279286.2
Latest version!
Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OGa43313 XM_015279287.2
Latest version!
Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OGa43314 XM_015279288.2
Latest version!
Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OGa43315 XM_015279289.2
Latest version!
Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OGa43316 XM_015279290.2
Latest version!
Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OGa43317 XM_015279291.2
Latest version!
Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OGa43318 XM_015279292.2
Latest version!
Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OGa43319 XM_015279293.2
Latest version!
Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OGa43320 XM_015279294.2
Latest version!
Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OGa27423 NM_001042538.1
Latest version!
Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
View Old Accession Versions >>
OGa43313 XM_015279287.1 Gallus gallus spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OGa43315 XM_015279289.1 Gallus gallus spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OGa43311 XM_015279285.1 Gallus gallus spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OGa43317 XM_015279291.1 Gallus gallus spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OGa43310 XM_015279284.1 Gallus gallus spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OGa43314 XM_015279288.1 Gallus gallus spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OGa43319 XM_015279293.1 Gallus gallus spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OGa43320 XM_015279294.1 Gallus gallus spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OGa43316 XM_015279290.1 Gallus gallus spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OGa43318 XM_015279292.1 Gallus gallus spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OGa43312 XM_015279286.1 Gallus gallus spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa43308
    Clone ID Related Accession (Same CDS sequence) XM_015279282.1
    Accession Version XM_015279282.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7602bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
    TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG
    TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC
    TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT
    GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT
    GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG
    ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAT CTTTCACGCC TTACAGGACT
    GCACAGCGCC CAATAGTAAC AGCCACCCAA GTTGGGTCCC ACATACGAAC TCGTCTACAG
    GAGCTGCACC GACTATGGGA GTTACTGCTG GAAAAAATGA GAGAGAAGGG AGTGAAGCTG
    TTGCAAGCAC AGAAGCTGGT GCAATTCTTA CGGGAATGTG AAGATGTCAT GGACTGGATC
    AATGACAAGG AAGCAATAGT GACCTCAGAA GAGCTTGGAC AGGACTTAGA GCATGTTGAG
    GTTTTGCAGA AGAAATTTGA AGAGTTCCAA ACAGATCTGG CAGCTCATGA GGAGAGAGTG
    AATGAAGTGA ACCAGTTCGC TGGCAAACTT ATCCAGGAAC AGCACCCTGA GGAGGAACTT
    ATAAAGTCCA AACAGGATGA AGTAAATGCA AGCTGGCAGC GTCTTAAGGG GCTTGCCCAA
    CAGAGGCAAG GAAAACTCTT TGGGGCAGCT GAAGTTCAGC GCTTCAACAG GGATGTGGAT
    GAAACTATCA GCTGGATTAA GGAGAAAGGG CAGTTGATGG CCTCAGATGA CTTTGGCAGA
    GACTTGGCCA GCGTGCAAGC TTTACTACGT AAGCATGAAG GCCTGGAAAG AGATCTTGCA
    GCTTTGGAGG ATAAGGTTAA GGCCCTCTGT GCAGAAGCTG ACCGTTTGCA GCAGTCTCAC
    CCTATAAATG CTTCTCAAAT TCAAGTGAAA CGGGAGGAAC TGATTGCCAA CTGGGAGCAG
    ATCCGAACTC TGGCAGCAGA GAGGCATGCT CGCCTTAATG ACTCCTACAG GTTGCAGCGC
    TTTCTTGCAG ATTTCCGGGA CCTCACTAGC TGGGTAACTG AGATGAAGGC TCTGATAAAT
    GCTGATGAGC TTGCCAATGA TGTGGCTGGA GCAGAAGCCC TTCTAGATAG ACATCAGGAA
    CATAAGGGAG AAATTGATGC TCATGAGGAT AGCTTCAGAT CTGCTGATGA GTCTGGGCAG
    GCTTTGCTTG CAGCTGGGCA CTATGCTTCT GATGAAGTTA AAGAAAAGCT GACTATCCTC
    TCAGATGAAA GATCAGCCTT GCTGGAACTA TGGGAGCTTC GCAGGCAACA GTATGAGCAG
    TGCATGGATC TGCAGCTTTT CTACAGAGAT ACTGAACAAG TTGACAACTG GATGAGCAAA
    CAAGAAGCTT TTCTGCTGAA CGAAGACCTT GGTGATTCTC TGGATAGCGT GGAGGCTCTT
    CTAAAGAAGC ATGAAGATTT TGAGAAATCC CTAAGTGCTC AAGAGGAGAA GATCACAGCA
    CTGGATGAGT TTGCTACTAA ACTGATTCAA AATAACCATT ATGCCATGGA TGACGTTGCT
    ACGCGCAGAG ATGCTCTTCT GAGTCGCCGA AATGCTCTTC ATGAAAGAGC CATGAAGCGC
    CGTGCCCAAC TGGCAGACTC TTTCCATCTC CAGCAGTTTT TCAGGGACTC TGATGAACTG
    AAGAGTTGGG TTAATGAAAA AATGAAAACT GCTACCGATG AGGCTTACAA GGATCCATCC
    AACTTGCAAG GTAAAGTTCA GAAGCATCAG GCTTTTGAAG CAGAGCTTTC TGCTAATCAG
    AGTCGTATTG ATGCACTGGA GAAAGCTGGC CAGAAGTTGA TTGATGTCAA TCACTATGCA
    TCTGATGAGG TGGCAGCTCG CATGAATGAA GTCATCAGCT TGTGGAAGAA ACTTCTGGAA
    GCCACCGAGC TCAAAGGTAT AAAGCTGCGT GAAGCCAATC AACAGCAGCA ATTTAATCGC
    AATGTAGAAG ACATTGAGTT GTGGCTGTAT GAAGTGGAGG GTCACTTGGC TTCTGATGAT
    TATGGAAAAG ATCTTACCAG CGTTCAGAAT CTTCAGAAGA AACATGCCCT GCTAGAGGCA
    GATGTTGCTG CCCATCAGGA TCGGATAGAT GGCATTACCA TCCAGGCACG CCAGTTCCAA
    GATGCTGGGC ACTTTGATGC TGACAATATC AAGAAGAAAC AAGAAGCTTT AGTAGCTCGT
    TATGAAGCTC TGAAGGATCC TATGGTGGCT CGCAAGCAGA AACTTGCAGA TTCTCTTCGC
    CTGCAGCAGC TTTTCCGTGA CATCGAGGAT GAAGAGACCT GGATCAGGGA AAAGGAACCT
    ATTGCAGCCT CAACAAACCG AGGCAAGGAC TTAATTGGTG TCCAGAATCT GCTAAAGAAG
    CACCAGGCTT TGCAGGCAGA AATTGCAGGC CATGAGCCTC GCATTAAAGC AGTCACACAA
    AAAGGAAATG CGATGGTGGA AGAGGGACAT TTTGCTGCTG AAGATGTGAA GATCAAATTG
    AATGAACTAA ACCAAAAATG GGACTCTCTG AAAGCCAAGG CATCTCAACG GCGACAGGAT
    CTAGAGGATT CTCTGCAAGC TCAGCAGTAT TTTGCTGATG CTAATGAGGC AGAATCATGG
    ATGAGGGAAA AGGAGCCCAT TGTAGGCAGC ACGGATTATG GAAAGGATGA AGACTCTGCT
    GAGGCTCTCC TGAAGAAGCA TGAAGCTTTG ATGTCTGATC TTTCTGCTTA CGGCAGTAGC
    ATACAGGCAT TAAGGGAACA GGCCCAGTCC TGCAGGCAAC AAGTTGCTCC CACTGATGAT
    GAAACTGGAA AAGAGCTTGT GCTAGCACTC TATGATTACC AAGAGAAGAG TCCTCGGGAG
    GTGACTATGA AGAAAGGAGA TATTCTAACC CTGCTCAACA GCACCAACAA GGACTGGTGG
    AAGGTTGAAG TTAACGATCG TCAGGGCTTT GTACCAGCTG CCTATGTGAA AAAACTAGAT
    CCTGCCCAGT CTGCATCCCG AGAGAATCTT CTAGAAGAGC AAGGCAGCAT AGCATTGCGA
    CAGGAGCAAA TTGACAACCA GACTCTCATT ACTAAGGAAG TCGGCAGTGT ATCTCTGCGT
    ATGAAACAGG TCGAAGAACT GTATCATTCC CTTCTTGAGC TGGGAGAAAA ACGTAAAGGC
    ATGCTAGAAA AAAGCTGCAA GAAGTTTATG CTTTTCCGTG AGGCTAATGA GCTTCAACAG
    TGGATCAATG AGAAGGAAGC TGCGCTCACT AATGAGGAAG TGGGTGCTGA TTTGGAGCAG
    GTGGAGGTGC TACAGAAGAA GTTTGATGAT TTTCAGAAGG ATTTAAAAGC TAATGAGTCA
    CGACTGAAGG ACATAAACAA GGTTGCAAAT GACCTGGAGT CTGAAGGGCT GATGGCAGAG
    GAAGTGCAAG CAGTAGAGCA CCAGGAAGTC TATGGAATGA TGCCCAGAGA TGAAACTGAT
    TCTAAGACAG CCTCTCCTTG GAAGTCTGCA CGTATGATGG TACACACAGT GGCAACGTTT
    AACTCAATCA AGGAACTGAA TGAACGCTGG AGATCCCTGC AGCAGTTGGC AGAGGAGCGA
    AGCCAGCTGT TGGGCAGCGC TCATGAAGTC CAGAGGTTCC ACAGAGATGC TGATGAAACA
    AAAGAATGGA TAGAAGAGAA GAATCAAGCA TTAAATACCG ACAACTATGG ACATGACTTG
    GCCAGTGTGC AGGCTCTGCA GCGCAAGCAT GAAGGCTTTG AGAGAGACTT GGCAGCTCTT
    GGAGACAAGG TGAATTCTCT TGGTGAAACT GCCCAGCGTC TGATCCAGTC ACATCCAGAA
    TCAGCTGAAG ATCTTCAAGA AAAATGTACT GAGTTGAACC AAGCCTGGAG TAGTCTGGGA
    AAACGTGCTG ACCAACGCAA AGAGAAGCTT GGAGATTCTC ATGACCTGCA GCGTTTCCTC
    AGTGATTTTA GAGACCTTAT GTCTTGGATC AATGGAATCC GGGGTCTGGT CTCCTCAGAT
    GAACTTGCAA AAGATGTGAC TGGAGCTGAA GCTCTGTTGG AAAGGCATCA GGAGCACCGT
    ACTGAAATAG ATGCACGGGC TGGCACTTTT CAGGCATTTG AACAGTTTGG ACAGCAGCTT
    CTGGCTCGTG GACACTATGC CAGCCCAGAG ATTAAGGAGA AACTGGATAT TCTAGATCAA
    GAACGGACAG ACCTAGAGAA GGCCTGGGTC CAGCGCAGAA TGATGCTAGA CCAGTGCTTA
    GAACTACAGC TGTTTCATCG GGATTGTGAA CAAGCTGAAA ACTGGATGGC TGCCCGAGAG
    GCGTTCCTAA ATACAGAAGA CAAAGGAGAC TCCTTAGACA GCGTGGAGGC ACTCATCAAG
    AAGCATGAAG ATTTCGATAA AGCAATCAAT GTCCAGGAAG AGAAAATTGC TGTCTTGCAG
    TCTTTTGCTG ACCAACTGAT TGCTGCTGAT CATTATGCAA AAGGAGTCAT TGCTAACAGA
    CGCAATGAGG TTCTGGACAG GTGGCGTCGT CTGAAGGCTC AGATGATTGA GAAGAGATCT
    AAGCTGGGAG AATCTCAGAC CCTCCAACAG TTCAGTCGTG ATGTTGATGA AATAGAAGCT
    TGGATCAGTG AAAAGCTTCA AACTGCAAGT GATGAGTCAT ATAAGGATCC CACAAACATC
    CAGCTTTCCA AACTGCTGAG CAAGCACCAG AAGCACCAAG CCTTTGAAGC TGAGCTCCAC
    GCCAACGCAG ATCGGATTCG TGGAGTCATT GAAATGGGGA ACTCTCTTAT TGAAAGAGGA
    GCGTGTGCTG GCAGCGAGGA TGCTGTGAAG GCTCGGCTGG CTGCCCTGGC TGACCAATGG
    GAGTTCCTGG TCCAGAAGTC ATCAGAGAAG AGTCAGAAAC TGAAAGAAGC AAATAAACAG
    CAGAATTTCA ATACCGGCAT CAAGGACTTT GATTTCTGGC TTTCAGAGGT GGAAGCTTTG
    TTGGCATCTG AAGACTATGG GAAGGACTTG GCATCAGTGA ACAACCTTCT GAAGAAGCAC
    CAATTACTGG AAGCTGATAT ATCTGCTCAT GAGGATCGGC TGAAGGACCT GAACAGCCAG
    GCTGACAGTT TGATGACCAG CAGTGCTTTC GATACCTCCC AAGTAAAGGA TAAACGTGAA
    ACTATAAATG GGCGCTTCCA GAGAATCAAG AGCATGGCAG CTGCCCGCCG TGCGAAGCTC
    AACGAGTCGC ACCGCTTGCA TCAGTTCTTC CGTGACATGG ATGATGAGGA GTCCTGGATC
    AAAGAGAAGA AACTGTTGGT GAGCTCAGAG GACTATGGCA GAGACCTGAC TGGTGTGCAG
    AACCTGAGGA AGAAACATAA GCGCTTGGAA GCAGAATTAG CTGCCCATGA ACCTGCTATC
    CAGGGTGTTC TAGACACTGG CAAGAAGCTT TCAGATGATA ACACAATTGG GAAGGAGGAG
    ATACAGCAAA GACTGGCTCA GTTTGTGGAC CACTGGAAAG AGTTAAAACA GCTGGCAGCT
    GCTCGGGGGC AGCGTCTGGA GGAGTCCCTG GAGTACCAAC AGTTTGTAGC AAATGTTGAG
    GAAGAGGAGG CATGGATCAA CGAGAAAATG ACATTGGTAG CCAGTGAGGA TTATGGAGAC
    ACACTTGCTG CTATCCAGGG CTTGCTGAAG AAGCATGAAG CATTCGAGAC TGACTTTACT
    GTCCACAAAG ACAGAGTGAA TGATGTTTGC GCTAATGGAG AGGATCTCAT TAAAAAGAAT
    AATCACCATG TGGAGAACAT TACTGCTAAG ATGAAGGGCC TCAAGGGCAA GGTATCAGAT
    CTGGAGAAAG CCGCAGCTCA GAGGAAAGCC AAACTGGATG AGAACTCTGC CTTCCTCCAG
    TTCAACTGGA AAGCAGATGT GGTGGAGTCA TGGATAGGTG AGAAGGAAAA CAGTCTGAAG
    ACAGATGATT ATGGCCGTGA CCTCTCCTCT GTGCAAACAC TACTCACCAA ACAGGAAACC
    TTTGATGCTG GACTTCAGGC TTTCCAGCAG GAGGGAATTG CAAACATCAC TGCTCTGAAA
    GACCAGCTAC TGGCAGCCAA ACATATCCAA TCAAAGGCCA TTGAGGTTCG TCACGCTTCC
    TTGATGAAAC GCTGGAATCA GCTGCTTGCT AATTCTGCAG CCAGGAAAAA GAAACTCTTG
    GAGGCTCAGG AGCACTTCAG AAAGGTTGAA GATCTCTTCT TGACTTTTGC CAAGAAGGCC
    TCTGCCTTCA ACAGTTGGTT TGAGAATGCT GAGGAGGACC TGACGGATCC CGTGCGCTGC
    AATTCCCTGG AAGAAATCAA AGCACTGCGA GAAGCCCACG ATGCGTTCCG TTCCTCACTT
    AGCTCTGCCC AAGCTGACTT CAACCAGCTG GCAGAGCTTG ATCGGCAGAT CAAGAGCTTC
    CGTGTAGCCT CCAACCCGTA CACTTGGTTT ACTATGGAGG CTCTTGAAGA AACTTGGAGG
    AATCTGCAGA AAATTATCAA GGAGCGTGAA TTGGAGTTGC AGAAGGAGCA GCGGAGGCAG
    GAAGAAAATG ACAAGTTGCG CCAGGAGTTT GCTCAGCATG CTAATGCCTT CCACCAGTGG
    ATTCAGGAGA CAAGGACTTA CCTGCTAGAT GGCATAGCCT ATCGTCGTGT CATTCGTGTC
    TATCAGTATG AAGTTGGGGA TGATCTATCT GGAAGGTCCT GCATGGTGGA GGAGTCGGGA
    ACACTGGAAT CCCAGCTGGA AGCTACTAAA CGCAAGCACC AGGAGATCCG GGCTATGAGG
    AGCCAGCTGA AGAAGATTGA GGACCTTGGA GCAGCCATGG AAGAGGCACT TATCTTGGAC
    AACAAATACA CAGAGCACAG CACCGTGGGG CTGGCGCAGC AGTGGGACCA GCTTGACCAG
    CTGGGAATGA GAATGCAGCA CAATCTGGAA CAGCAGATTC AAGCTCGAAA CACAACTGGA
    GTCACAGAGG AGGCCCTGAA GGAGTTCAGC ATGATGTTCA AGCACTTTGA CAAGGACAAA
    TCTGGACGTC TTAATCACCA GGAGTTTAAG TCTTGCTTGC GCTCCCTTGG CTATGACCTG
    CCCATGGTTG AGGAAGGAGA GCCTGACCCC GAATTTGAGT CTATTCTTGA CACTGTTGAT
    CCCAACAGGG ATGGCCACGT CTCGCTGCAG GAGTACATGG CGTTCATGAT CAGCAGGGAA
    ACAGAGAACG TGAAATCCAG CGAGGAGATT GAGAGTGCTT TCCGTGCCCT CAGCTCGGAG
    GGGAAGCCTT ACGTGACCAA GGAGGAGCTC TACCAGAACC TGACCCGGGA GCAGGCCGAC
    TACTGCATTT CTCACATGAA GCCCTACATG GATGGCAAGG GCAGAGAACT TCCTTCTGCC
    TACGACTACA TAGAATTTAC ACGTTCACTC TTTGTGAATT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015134768.1
    CDS19..7620
    Translation

    Target ORF information:

    RefSeq Version XM_015279282.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015279282.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
    TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG
    TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC
    TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT
    GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT
    GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG
    ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAT CTTTCACGCC TTACAGGACT
    GCACAGCGCC CAATAGTAAC AGCCACCCAA GTTGGGTCCC ACATACGAAC TCGTCTACAG
    GAGCTGCACC GACTATGGGA GTTACTGCTG GAAAAAATGA GAGAGAAGGG AGTGAAGCTG
    TTGCAAGCAC AGAAGCTGGT GCAATTCTTA CGGGAATGTG AAGATGTCAT GGACTGGATC
    AATGACAAGG AAGCAATAGT GACCTCAGAA GAGCTTGGAC AGGACTTAGA GCATGTTGAG
    GTTTTGCAGA AGAAATTTGA AGAGTTCCAA ACAGATCTGG CAGCTCATGA GGAGAGAGTG
    AATGAAGTGA ACCAGTTCGC TGGCAAACTT ATCCAGGAAC AGCACCCTGA GGAGGAACTT
    ATAAAGTCCA AACAGGATGA AGTAAATGCA AGCTGGCAGC GTCTTAAGGG GCTTGCCCAA
    CAGAGGCAAG GAAAACTCTT TGGGGCAGCT GAAGTTCAGC GCTTCAACAG GGATGTGGAT
    GAAACTATCA GCTGGATTAA GGAGAAAGGG CAGTTGATGG CCTCAGATGA CTTTGGCAGA
    GACTTGGCCA GCGTGCAAGC TTTACTACGT AAGCATGAAG GCCTGGAAAG AGATCTTGCA
    GCTTTGGAGG ATAAGGTTAA GGCCCTCTGT GCAGAAGCTG ACCGTTTGCA GCAGTCTCAC
    CCTATAAATG CTTCTCAAAT TCAAGTGAAA CGGGAGGAAC TGATTGCCAA CTGGGAGCAG
    ATCCGAACTC TGGCAGCAGA GAGGCATGCT CGCCTTAATG ACTCCTACAG GTTGCAGCGC
    TTTCTTGCAG ATTTCCGGGA CCTCACTAGC TGGGTAACTG AGATGAAGGC TCTGATAAAT
    GCTGATGAGC TTGCCAATGA TGTGGCTGGA GCAGAAGCCC TTCTAGATAG ACATCAGGAA
    CATAAGGGAG AAATTGATGC TCATGAGGAT AGCTTCAGAT CTGCTGATGA GTCTGGGCAG
    GCTTTGCTTG CAGCTGGGCA CTATGCTTCT GATGAAGTTA AAGAAAAGCT GACTATCCTC
    TCAGATGAAA GATCAGCCTT GCTGGAACTA TGGGAGCTTC GCAGGCAACA GTATGAGCAG
    TGCATGGATC TGCAGCTTTT CTACAGAGAT ACTGAACAAG TTGACAACTG GATGAGCAAA
    CAAGAAGCTT TTCTGCTGAA CGAAGACCTT GGTGATTCTC TGGATAGCGT GGAGGCTCTT
    CTAAAGAAGC ATGAAGATTT TGAGAAATCC CTAAGTGCTC AAGAGGAGAA GATCACAGCA
    CTGGATGAGT TTGCTACTAA ACTGATTCAA AATAACCATT ATGCCATGGA TGACGTTGCT
    ACGCGCAGAG ATGCTCTTCT GAGTCGCCGA AATGCTCTTC ATGAAAGAGC CATGAAGCGC
    CGTGCCCAAC TGGCAGACTC TTTCCATCTC CAGCAGTTTT TCAGGGACTC TGATGAACTG
    AAGAGTTGGG TTAATGAAAA AATGAAAACT GCTACCGATG AGGCTTACAA GGATCCATCC
    AACTTGCAAG GTAAAGTTCA GAAGCATCAG GCTTTTGAAG CAGAGCTTTC TGCTAATCAG
    AGTCGTATTG ATGCACTGGA GAAAGCTGGC CAGAAGTTGA TTGATGTCAA TCACTATGCA
    TCTGATGAGG TGGCAGCTCG CATGAATGAA GTCATCAGCT TGTGGAAGAA ACTTCTGGAA
    GCCACCGAGC TCAAAGGTAT AAAGCTGCGT GAAGCCAATC AACAGCAGCA ATTTAATCGC
    AATGTAGAAG ACATTGAGTT GTGGCTGTAT GAAGTGGAGG GTCACTTGGC TTCTGATGAT
    TATGGAAAAG ATCTTACCAG CGTTCAGAAT CTTCAGAAGA AACATGCCCT GCTAGAGGCA
    GATGTTGCTG CCCATCAGGA TCGGATAGAT GGCATTACCA TCCAGGCACG CCAGTTCCAA
    GATGCTGGGC ACTTTGATGC TGACAATATC AAGAAGAAAC AAGAAGCTTT AGTAGCTCGT
    TATGAAGCTC TGAAGGATCC TATGGTGGCT CGCAAGCAGA AACTTGCAGA TTCTCTTCGC
    CTGCAGCAGC TTTTCCGTGA CATCGAGGAT GAAGAGACCT GGATCAGGGA AAAGGAACCT
    ATTGCAGCCT CAACAAACCG AGGCAAGGAC TTAATTGGTG TCCAGAATCT GCTAAAGAAG
    CACCAGGCTT TGCAGGCAGA AATTGCAGGC CATGAGCCTC GCATTAAAGC AGTCACACAA
    AAAGGAAATG CGATGGTGGA AGAGGGACAT TTTGCTGCTG AAGATGTGAA GATCAAATTG
    AATGAACTAA ACCAAAAATG GGACTCTCTG AAAGCCAAGG CATCTCAACG GCGACAGGAT
    CTAGAGGATT CTCTGCAAGC TCAGCAGTAT TTTGCTGATG CTAATGAGGC AGAATCATGG
    ATGAGGGAAA AGGAGCCCAT TGTAGGCAGC ACGGATTATG GAAAGGATGA AGACTCTGCT
    GAGGCTCTCC TGAAGAAGCA TGAAGCTTTG ATGTCTGATC TTTCTGCTTA CGGCAGTAGC
    ATACAGGCAT TAAGGGAACA GGCCCAGTCC TGCAGGCAAC AAGTTGCTCC CACTGATGAT
    GAAACTGGAA AAGAGCTTGT GCTAGCACTC TATGATTACC AAGAGAAGAG TCCTCGGGAG
    GTGACTATGA AGAAAGGAGA TATTCTAACC CTGCTCAACA GCACCAACAA GGACTGGTGG
    AAGGTTGAAG TTAACGATCG TCAGGGCTTT GTACCAGCTG CCTATGTGAA AAAACTAGAT
    CCTGCCCAGT CTGCATCCCG AGAGAATCTT CTAGAAGAGC AAGGCAGCAT AGCATTGCGA
    CAGGAGCAAA TTGACAACCA GACTCTCATT ACTAAGGAAG TCGGCAGTGT ATCTCTGCGT
    ATGAAACAGG TCGAAGAACT GTATCATTCC CTTCTTGAGC TGGGAGAAAA ACGTAAAGGC
    ATGCTAGAAA AAAGCTGCAA GAAGTTTATG CTTTTCCGTG AGGCTAATGA GCTTCAACAG
    TGGATCAATG AGAAGGAAGC TGCGCTCACT AATGAGGAAG TGGGTGCTGA TTTGGAGCAG
    GTGGAGGTGC TACAGAAGAA GTTTGATGAT TTTCAGAAGG ATTTAAAAGC TAATGAGTCA
    CGACTGAAGG ACATAAACAA GGTTGCAAAT GACCTGGAGT CTGAAGGGCT GATGGCAGAG
    GAAGTGCAAG CAGTAGAGCA CCAGGAAGTC TATGGAATGA TGCCCAGAGA TGAAACTGAT
    TCTAAGACAG CCTCTCCTTG GAAGTCTGCA CGTATGATGG TACACACAGT GGCAACGTTT
    AACTCAATCA AGGAACTGAA TGAACGCTGG AGATCCCTGC AGCAGTTGGC AGAGGAGCGA
    AGCCAGCTGT TGGGCAGCGC TCATGAAGTC CAGAGGTTCC ACAGAGATGC TGATGAAACA
    AAAGAATGGA TAGAAGAGAA GAATCAAGCA TTAAATACCG ACAACTATGG ACATGACTTG
    GCCAGTGTGC AGGCTCTGCA GCGCAAGCAT GAAGGCTTTG AGAGAGACTT GGCAGCTCTT
    GGAGACAAGG TGAATTCTCT TGGTGAAACT GCCCAGCGTC TGATCCAGTC ACATCCAGAA
    TCAGCTGAAG ATCTTCAAGA AAAATGTACT GAGTTGAACC AAGCCTGGAG TAGTCTGGGA
    AAACGTGCTG ACCAACGCAA AGAGAAGCTT GGAGATTCTC ATGACCTGCA GCGTTTCCTC
    AGTGATTTTA GAGACCTTAT GTCTTGGATC AATGGAATCC GGGGTCTGGT CTCCTCAGAT
    GAACTTGCAA AAGATGTGAC TGGAGCTGAA GCTCTGTTGG AAAGGCATCA GGAGCACCGT
    ACTGAAATAG ATGCACGGGC TGGCACTTTT CAGGCATTTG AACAGTTTGG ACAGCAGCTT
    CTGGCTCGTG GACACTATGC CAGCCCAGAG ATTAAGGAGA AACTGGATAT TCTAGATCAA
    GAACGGACAG ACCTAGAGAA GGCCTGGGTC CAGCGCAGAA TGATGCTAGA CCAGTGCTTA
    GAACTACAGC TGTTTCATCG GGATTGTGAA CAAGCTGAAA ACTGGATGGC TGCCCGAGAG
    GCGTTCCTAA ATACAGAAGA CAAAGGAGAC TCCTTAGACA GCGTGGAGGC ACTCATCAAG
    AAGCATGAAG ATTTCGATAA AGCAATCAAT GTCCAGGAAG AGAAAATTGC TGTCTTGCAG
    TCTTTTGCTG ACCAACTGAT TGCTGCTGAT CATTATGCAA AAGGAGTCAT TGCTAACAGA
    CGCAATGAGG TTCTGGACAG GTGGCGTCGT CTGAAGGCTC AGATGATTGA GAAGAGATCT
    AAGCTGGGAG AATCTCAGAC CCTCCAACAG TTCAGTCGTG ATGTTGATGA AATAGAAGCT
    TGGATCAGTG AAAAGCTTCA AACTGCAAGT GATGAGTCAT ATAAGGATCC CACAAACATC
    CAGCTTTCCA AACTGCTGAG CAAGCACCAG AAGCACCAAG CCTTTGAAGC TGAGCTCCAC
    GCCAACGCAG ATCGGATTCG TGGAGTCATT GAAATGGGGA ACTCTCTTAT TGAAAGAGGA
    GCGTGTGCTG GCAGCGAGGA TGCTGTGAAG GCTCGGCTGG CTGCCCTGGC TGACCAATGG
    GAGTTCCTGG TCCAGAAGTC ATCAGAGAAG AGTCAGAAAC TGAAAGAAGC AAATAAACAG
    CAGAATTTCA ATACCGGCAT CAAGGACTTT GATTTCTGGC TTTCAGAGGT GGAAGCTTTG
    TTGGCATCTG AAGACTATGG GAAGGACTTG GCATCAGTGA ACAACCTTCT GAAGAAGCAC
    CAATTACTGG AAGCTGATAT ATCTGCTCAT GAGGATCGGC TGAAGGACCT GAACAGCCAG
    GCTGACAGTT TGATGACCAG CAGTGCTTTC GATACCTCCC AAGTAAAGGA TAAACGTGAA
    ACTATAAATG GGCGCTTCCA GAGAATCAAG AGCATGGCAG CTGCCCGCCG TGCGAAGCTC
    AACGAGTCGC ACCGCTTGCA TCAGTTCTTC CGTGACATGG ATGATGAGGA GTCCTGGATC
    AAAGAGAAGA AACTGTTGGT GAGCTCAGAG GACTATGGCA GAGACCTGAC TGGTGTGCAG
    AACCTGAGGA AGAAACATAA GCGCTTGGAA GCAGAATTAG CTGCCCATGA ACCTGCTATC
    CAGGGTGTTC TAGACACTGG CAAGAAGCTT TCAGATGATA ACACAATTGG GAAGGAGGAG
    ATACAGCAAA GACTGGCTCA GTTTGTGGAC CACTGGAAAG AGTTAAAACA GCTGGCAGCT
    GCTCGGGGGC AGCGTCTGGA GGAGTCCCTG GAGTACCAAC AGTTTGTAGC AAATGTTGAG
    GAAGAGGAGG CATGGATCAA CGAGAAAATG ACATTGGTAG CCAGTGAGGA TTATGGAGAC
    ACACTTGCTG CTATCCAGGG CTTGCTGAAG AAGCATGAAG CATTCGAGAC TGACTTTACT
    GTCCACAAAG ACAGAGTGAA TGATGTTTGC GCTAATGGAG AGGATCTCAT TAAAAAGAAT
    AATCACCATG TGGAGAACAT TACTGCTAAG ATGAAGGGCC TCAAGGGCAA GGTATCAGAT
    CTGGAGAAAG CCGCAGCTCA GAGGAAAGCC AAACTGGATG AGAACTCTGC CTTCCTCCAG
    TTCAACTGGA AAGCAGATGT GGTGGAGTCA TGGATAGGTG AGAAGGAAAA CAGTCTGAAG
    ACAGATGATT ATGGCCGTGA CCTCTCCTCT GTGCAAACAC TACTCACCAA ACAGGAAACC
    TTTGATGCTG GACTTCAGGC TTTCCAGCAG GAGGGAATTG CAAACATCAC TGCTCTGAAA
    GACCAGCTAC TGGCAGCCAA ACATATCCAA TCAAAGGCCA TTGAGGTTCG TCACGCTTCC
    TTGATGAAAC GCTGGAATCA GCTGCTTGCT AATTCTGCAG CCAGGAAAAA GAAACTCTTG
    GAGGCTCAGG AGCACTTCAG AAAGGTTGAA GATCTCTTCT TGACTTTTGC CAAGAAGGCC
    TCTGCCTTCA ACAGTTGGTT TGAGAATGCT GAGGAGGACC TGACGGATCC CGTGCGCTGC
    AATTCCCTGG AAGAAATCAA AGCACTGCGA GAAGCCCACG ATGCGTTCCG TTCCTCACTT
    AGCTCTGCCC AAGCTGACTT CAACCAGCTG GCAGAGCTTG ATCGGCAGAT CAAGAGCTTC
    CGTGTAGCCT CCAACCCGTA CACTTGGTTT ACTATGGAGG CTCTTGAAGA AACTTGGAGG
    AATCTGCAGA AAATTATCAA GGAGCGTGAA TTGGAGTTGC AGAAGGAGCA GCGGAGGCAG
    GAAGAAAATG ACAAGTTGCG CCAGGAGTTT GCTCAGCATG CTAATGCCTT CCACCAGTGG
    ATTCAGGAGA CAAGGACTTA CCTGCTAGAT GGCATAGCCT ATCGTCGTGT CATTCGTGTC
    TATCAGTATG AAGTTGGGGA TGATCTATCT GGAAGGTCCT GCATGGTGGA GGAGTCGGGA
    ACACTGGAAT CCCAGCTGGA AGCTACTAAA CGCAAGCACC AGGAGATCCG GGCTATGAGG
    AGCCAGCTGA AGAAGATTGA GGACCTTGGA GCAGCCATGG AAGAGGCACT TATCTTGGAC
    AACAAATACA CAGAGCACAG CACCGTGGGG CTGGCGCAGC AGTGGGACCA GCTTGACCAG
    CTGGGAATGA GAATGCAGCA CAATCTGGAA CAGCAGATTC AAGCTCGAAA CACAACTGGA
    GTCACAGAGG AGGCCCTGAA GGAGTTCAGC ATGATGTTCA AGCACTTTGA CAAGGACAAA
    TCTGGACGTC TTAATCACCA GGAGTTTAAG TCTTGCTTGC GCTCCCTTGG CTATGACCTG
    CCCATGGTTG AGGAAGGAGA GCCTGACCCC GAATTTGAGT CTATTCTTGA CACTGTTGAT
    CCCAACAGGG ATGGCCACGT CTCGCTGCAG GAGTACATGG CGTTCATGAT CAGCAGGGAA
    ACAGAGAACG TGAAATCCAG CGAGGAGATT GAGAGTGCTT TCCGTGCCCT CAGCTCGGAG
    GGGAAGCCTT ACGTGACCAA GGAGGAGCTC TACCAGAACC TGACCCGGGA GCAGGCCGAC
    TACTGCATTT CTCACATGAA GCCCTACATG GATGGCAAGG GCAGAGAACT TCCTTCTGCC
    TACGACTACA TAGAATTTAC ACGTTCACTC TTTGTGAATT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa43309
    Clone ID Related Accession (Same CDS sequence) XM_015279283.1
    Accession Version XM_015279283.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7587bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
    TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG
    TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC
    TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT
    GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT
    GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG
    ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAT CTTTCACGCC TTACAGGACT
    GCACAGCGCC CAATAGTAAC AGCCACCCAA GTTGGGTCCC ACATACGAAC TCGTCTACAG
    GAGCTGCACC GACTATGGGA GTTACTGCTG GAAAAAATGA GAGAGAAGGG AGTGAAGCTG
    TTGCAAGCAC AGAAGCTGGT GCAATTCTTA CGGGAATGTG AAGATGTCAT GGACTGGATC
    AATGACAAGG AAGCAATAGT GACCTCAGAA GAGCTTGGAC AGGACTTAGA GCATGTTGAG
    GTTTTGCAGA AGAAATTTGA AGAGTTCCAA ACAGATCTGG CAGCTCATGA GGAGAGAGTG
    AATGAAGTGA ACCAGTTCGC TGGCAAACTT ATCCAGGAAC AGCACCCTGA GGAGGAACTT
    ATAAAGTCCA AACAGGATGA AGTAAATGCA AGCTGGCAGC GTCTTAAGGG GCTTGCCCAA
    CAGAGGCAAG GAAAACTCTT TGGGGCAGCT GAAGTTCAGC GCTTCAACAG GGATGTGGAT
    GAAACTATCA GCTGGATTAA GGAGAAAGGG CAGTTGATGG CCTCAGATGA CTTTGGCAGA
    GACTTGGCCA GCGTGCAAGC TTTACTACGT AAGCATGAAG GCCTGGAAAG AGATCTTGCA
    GCTTTGGAGG ATAAGGTTAA GGCCCTCTGT GCAGAAGCTG ACCGTTTGCA GCAGTCTCAC
    CCTATAAATG CTTCTCAAAT TCAAGTGAAA CGGGAGGAAC TGATTGCCAA CTGGGAGCAG
    ATCCGAACTC TGGCAGCAGA GAGGCATGCT CGCCTTAATG ACTCCTACAG GTTGCAGCGC
    TTTCTTGCAG ATTTCCGGGA CCTCACTAGC TGGGTAACTG AGATGAAGGC TCTGATAAAT
    GCTGATGAGC TTGCCAATGA TGTGGCTGGA GCAGAAGCCC TTCTAGATAG ACATCAGGAA
    CATAAGGGAG AAATTGATGC TCATGAGGAT AGCTTCAGAT CTGCTGATGA GTCTGGGCAG
    GCTTTGCTTG CAGCTGGGCA CTATGCTTCT GATGAAGTTA AAGAAAAGCT GACTATCCTC
    TCAGATGAAA GATCAGCCTT GCTGGAACTA TGGGAGCTTC GCAGGCAACA GTATGAGCAG
    TGCATGGATC TGCAGCTTTT CTACAGAGAT ACTGAACAAG TTGACAACTG GATGAGCAAA
    CAAGAAGCTT TTCTGCTGAA CGAAGACCTT GGTGATTCTC TGGATAGCGT GGAGGCTCTT
    CTAAAGAAGC ATGAAGATTT TGAGAAATCC CTAAGTGCTC AAGAGGAGAA GATCACAGCA
    CTGGATGAGT TTGCTACTAA ACTGATTCAA AATAACCATT ATGCCATGGA TGACGTTGCT
    ACGCGCAGAG ATGCTCTTCT GAGTCGCCGA AATGCTCTTC ATGAAAGAGC CATGAAGCGC
    CGTGCCCAAC TGGCAGACTC TTTCCATCTC CAGCAGTTTT TCAGGGACTC TGATGAACTG
    AAGAGTTGGG TTAATGAAAA AATGAAAACT GCTACCGATG AGGCTTACAA GGATCCATCC
    AACTTGCAAG GTAAAGTTCA GAAGCATCAG GCTTTTGAAG CAGAGCTTTC TGCTAATCAG
    AGTCGTATTG ATGCACTGGA GAAAGCTGGC CAGAAGTTGA TTGATGTCAA TCACTATGCA
    TCTGATGAGG TGGCAGCTCG CATGAATGAA GTCATCAGCT TGTGGAAGAA ACTTCTGGAA
    GCCACCGAGC TCAAAGGTAT AAAGCTGCGT GAAGCCAATC AACAGCAGCA ATTTAATCGC
    AATGTAGAAG ACATTGAGTT GTGGCTGTAT GAAGTGGAGG GTCACTTGGC TTCTGATGAT
    TATGGAAAAG ATCTTACCAG CGTTCAGAAT CTTCAGAAGA AACATGCCCT GCTAGAGGCA
    GATGTTGCTG CCCATCAGGA TCGGATAGAT GGCATTACCA TCCAGGCACG CCAGTTCCAA
    GATGCTGGGC ACTTTGATGC TGACAATATC AAGAAGAAAC AAGAAGCTTT AGTAGCTCGT
    TATGAAGCTC TGAAGGATCC TATGGTGGCT CGCAAGCAGA AACTTGCAGA TTCTCTTCGC
    CTGCAGCAGC TTTTCCGTGA CATCGAGGAT GAAGAGACCT GGATCAGGGA AAAGGAACCT
    ATTGCAGCCT CAACAAACCG AGGCAAGGAC TTAATTGGTG TCCAGAATCT GCTAAAGAAG
    CACCAGGCTT TGCAGGCAGA AATTGCAGGC CATGAGCCTC GCATTAAAGC AGTCACACAA
    AAAGGAAATG CGATGGTGGA AGAGGGACAT TTTGCTGCTG AAGATGTGAA GATCAAATTG
    AATGAACTAA ACCAAAAATG GGACTCTCTG AAAGCCAAGG CATCTCAACG GCGACAGGAT
    CTAGAGGATT CTCTGCAAGC TCAGCAGTAT TTTGCTGATG CTAATGAGGC AGAATCATGG
    ATGAGGGAAA AGGAGCCCAT TGTAGGCAGC ACGGATTATG GAAAGGATGA AGACTCTGCT
    GAGGCTCTCC TGAAGAAGCA TGAAGCTTTG ATGTCTGATC TTTCTGCTTA CGGCAGTAGC
    ATACAGGCAT TAAGGGAACA GGCCCAGTCC TGCAGGCAAC AAGTTGCTCC CACTGATGAT
    GAAACTGGAA AAGAGCTTGT GCTAGCACTC TATGATTACC AAGAGAAGAG TCCTCGGGAG
    GTGACTATGA AGAAAGGAGA TATTCTAACC CTGCTCAACA GCACCAACAA GGACTGGTGG
    AAGGTTGAAG TTAACGATCG TCAGGGCTTT GTACCAGCTG CCTATGTGAA AAAACTAGAT
    CCTGCCCAGT CTGCATCCCG AGAGAATCTT CTAGAAGAGC AAGGCAGCAT AGCATTGCGA
    CAGGAGCAAA TTGACAACCA GACTCTCATT ACTAAGGAAG TCGGCAGTGT ATCTCTGCGT
    ATGAAACAGG TCGAAGAACT GTATCATTCC CTTCTTGAGC TGGGAGAAAA ACGTAAAGGC
    ATGCTAGAAA AAAGCTGCAA GAAGTTTATG CTTTTCCGTG AGGCTAATGA GCTTCAACAG
    TGGATCAATG AGAAGGAAGC TGCGCTCACT AATGAGGAAG TGGGTGCTGA TTTGGAGCAG
    GTGGAGGTGC TACAGAAGAA GTTTGATGAT TTTCAGAAGG ATTTAAAAGC TAATGAGTCA
    CGACTGAAGG ACATAAACAA GGTTGCAAAT GACCTGGAGT CTGAAGGGCT GATGGCAGAG
    GAAGTGCAAG CAGTAGAGCA CCAGGAAGTC TATGGAATGA TGCCCAGAGA TGAAACTGAT
    TCTAAGACAG CCTCTCCTTG GAAGTCTGCA CGTATGATGG TACACACAGT GGCAACGTTT
    AACTCAATCA AGGAACTGAA TGAACGCTGG AGATCCCTGC AGCAGTTGGC AGAGGAGCGA
    AGCCAGCTGT TGGGCAGCGC TCATGAAGTC CAGAGGTTCC ACAGAGATGC TGATGAAACA
    AAAGAATGGA TAGAAGAGAA GAATCAAGCA TTAAATACCG ACAACTATGG ACATGACTTG
    GCCAGTGTGC AGGCTCTGCA GCGCAAGCAT GAAGGCTTTG AGAGAGACTT GGCAGCTCTT
    GGAGACAAGG TGAATTCTCT TGGTGAAACT GCCCAGCGTC TGATCCAGTC ACATCCAGAA
    TCAGCTGAAG ATCTTCAAGA AAAATGTACT GAGTTGAACC AAGCCTGGAG TAGTCTGGGA
    AAACGTGCTG ACCAACGCAA AGAGAAGCTT GGAGATTCTC ATGACCTGCA GCGTTTCCTC
    AGTGATTTTA GAGACCTTAT GTCTTGGATC AATGGAATCC GGGGTCTGGT CTCCTCAGAT
    GAACTTGCAA AAGATGTGAC TGGAGCTGAA GCTCTGTTGG AAAGGCATCA GGAGCACCGT
    ACTGAAATAG ATGCACGGGC TGGCACTTTT CAGGCATTTG AACAGTTTGG ACAGCAGCTT
    CTGGCTCGTG GACACTATGC CAGCCCAGAG ATTAAGGAGA AACTGGATAT TCTAGATCAA
    GAACGGACAG ACCTAGAGAA GGCCTGGGTC CAGCGCAGAA TGATGCTAGA CCAGTGCTTA
    GAACTACAGC TGTTTCATCG GGATTGTGAA CAAGCTGAAA ACTGGATGGC TGCCCGAGAG
    GCGTTCCTAA ATACAGAAGA CAAAGGAGAC TCCTTAGACA GCGTGGAGGC ACTCATCAAG
    AAGCATGAAG ATTTCGATAA AGCAATCAAT GTCCAGGAAG AGAAAATTGC TGTCTTGCAG
    TCTTTTGCTG ACCAACTGAT TGCTGCTGAT CATTATGCAA AAGGAGTCAT TGCTAACAGA
    CGCAATGAGG TTCTGGACAG GTGGCGTCGT CTGAAGGCTC AGATGATTGA GAAGAGATCT
    AAGCTGGGAG AATCTCAGAC CCTCCAACAG TTCAGTCGTG ATGTTGATGA AATAGAAGCT
    TGGATCAGTG AAAAGCTTCA AACTGCAAGT GATGAGTCAT ATAAGGATCC CACAAACATC
    CAGAGCAAGC ACCAGAAGCA CCAAGCCTTT GAAGCTGAGC TCCACGCCAA CGCAGATCGG
    ATTCGTGGAG TCATTGAAAT GGGGAACTCT CTTATTGAAA GAGGAGCGTG TGCTGGCAGC
    GAGGATGCTG TGAAGGCTCG GCTGGCTGCC CTGGCTGACC AATGGGAGTT CCTGGTCCAG
    AAGTCATCAG AGAAGAGTCA GAAACTGAAA GAAGCAAATA AACAGCAGAA TTTCAATACC
    GGCATCAAGG ACTTTGATTT CTGGCTTTCA GAGGTGGAAG CTTTGTTGGC ATCTGAAGAC
    TATGGGAAGG ACTTGGCATC AGTGAACAAC CTTCTGAAGA AGCACCAATT ACTGGAAGCT
    GATATATCTG CTCATGAGGA TCGGCTGAAG GACCTGAACA GCCAGGCTGA CAGTTTGATG
    ACCAGCAGTG CTTTCGATAC CTCCCAAGTA AAGGATAAAC GTGAAACTAT AAATGGGCGC
    TTCCAGAGAA TCAAGAGCAT GGCAGCTGCC CGCCGTGCGA AGCTCAACGA GTCGCACCGC
    TTGCATCAGT TCTTCCGTGA CATGGATGAT GAGGAGTCCT GGATCAAAGA GAAGAAACTG
    TTGGTGAGCT CAGAGGACTA TGGCAGAGAC CTGACTGGTG TGCAGAACCT GAGGAAGAAA
    CATAAGCGCT TGGAAGCAGA ATTAGCTGCC CATGAACCTG CTATCCAGGG TGTTCTAGAC
    ACTGGCAAGA AGCTTTCAGA TGATAACACA ATTGGGAAGG AGGAGATACA GCAAAGACTG
    GCTCAGTTTG TGGACCACTG GAAAGAGTTA AAACAGCTGG CAGCTGCTCG GGGGCAGCGT
    CTGGAGGAGT CCCTGGAGTA CCAACAGTTT GTAGCAAATG TTGAGGAAGA GGAGGCATGG
    ATCAACGAGA AAATGACATT GGTAGCCAGT GAGGATTATG GAGACACACT TGCTGCTATC
    CAGGGCTTGC TGAAGAAGCA TGAAGCATTC GAGACTGACT TTACTGTCCA CAAAGACAGA
    GTGAATGATG TTTGCGCTAA TGGAGAGGAT CTCATTAAAA AGAATAATCA CCATGTGGAG
    AACATTACTG CTAAGATGAA GGGCCTCAAG GGCAAGGTAT CAGATCTGGA GAAAGCCGCA
    GCTCAGAGGA AAGCCAAACT GGATGAGAAC TCTGCCTTCC TCCAGTTCAA CTGGAAAGCA
    GATGTGGTGG AGTCATGGAT AGGTGAGAAG GAAAACAGTC TGAAGACAGA TGATTATGGC
    CGTGACCTCT CCTCTGTGCA AACACTACTC ACCAAACAGG AAACCTTTGA TGCTGGACTT
    CAGGCTTTCC AGCAGGAGGG AATTGCAAAC ATCACTGCTC TGAAAGACCA GCTACTGGCA
    GCCAAACATA TCCAATCAAA GGCCATTGAG GTTCGTCACG CTTCCTTGAT GAAACGCTGG
    AATCAGCTGC TTGCTAATTC TGCAGCCAGG AAAAAGAAAC TCTTGGAGGC TCAGGAGCAC
    TTCAGAAAGG TTGAAGATCT CTTCTTGACT TTTGCCAAGA AGGCCTCTGC CTTCAACAGT
    TGGTTTGAGA ATGCTGAGGA GGACCTGACG GATCCCGTGC GCTGCAATTC CCTGGAAGAA
    ATCAAAGCAC TGCGAGAAGC CCACGATGCG TTCCGTTCCT CACTTAGCTC TGCCCAAGCT
    GACTTCAACC AGCTGGCAGA GCTTGATCGG CAGATCAAGA GCTTCCGTGT AGCCTCCAAC
    CCGTACACTT GGTTTACTAT GGAGGCTCTT GAAGAAACTT GGAGGAATCT GCAGAAAATT
    ATCAAGGAGC GTGAATTGGA GTTGCAGAAG GAGCAGCGGA GGCAGGAAGA AAATGACAAG
    TTGCGCCAGG AGTTTGCTCA GCATGCTAAT GCCTTCCACC AGTGGATTCA GGAGACAAGG
    ACTTACCTGC TAGATGGCAT AGCCTATCGT CGTGTCATTC GTGTCTATCA GTATGAAGTT
    GGGGATGATC TATCTGGAAG GTCCTGCATG GTGGAGGAGT CGGGAACACT GGAATCCCAG
    CTGGAAGCTA CTAAACGCAA GCACCAGGAG ATCCGGGCTA TGAGGAGCCA GCTGAAGAAG
    ATTGAGGACC TTGGAGCAGC CATGGAAGAG GCACTTATCT TGGACAACAA ATACACAGAG
    CACAGCACCG TGGGGCTGGC GCAGCAGTGG GACCAGCTTG ACCAGCTGGG AATGAGAATG
    CAGCACAATC TGGAACAGCA GATTCAAGCT CGAAACACAA CTGGAGTCAC AGAGGAGGCC
    CTGAAGGAGT TCAGCATGAT GTTCAAGCAC TTTGACAAGG ACAAATCTGG ACGTCTTAAT
    CACCAGGAGT TTAAGTCTTG CTTGCGCTCC CTTGGCTATG ACCTGCCCAT GGTTGAGGAA
    GGAGAGCCTG ACCCCGAATT TGAGTCTATT CTTGACACTG TTGATCCCAA CAGGGATGGC
    CACGTCTCGC TGCAGGAGTA CATGGCGTTC ATGATCAGCA GGGAAACAGA GAACGTGAAA
    TCCAGCGAGG AGATTGAGAG TGCTTTCCGT GCCCTCAGCT CGGAGGGGAA GCCTTACGTG
    ACCAAGGAGG AGCTCTACCA GAACCTGACC CGGGAGCAGG CCGACTACTG CATTTCTCAC
    ATGAAGCCCT ACATGGATGG CAAGGGCAGA GAACTTCCTT CTGCCTACGA CTACATAGAA
    TTTACACGTT CACTCTTTGT GAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015134769.1
    CDS18..7604
    Translation

    Target ORF information:

    RefSeq Version XM_015279283.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015279283.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
    TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG
    TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC
    TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT
    GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT
    GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG
    ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAT CTTTCACGCC TTACAGGACT
    GCACAGCGCC CAATAGTAAC AGCCACCCAA GTTGGGTCCC ACATACGAAC TCGTCTACAG
    GAGCTGCACC GACTATGGGA GTTACTGCTG GAAAAAATGA GAGAGAAGGG AGTGAAGCTG
    TTGCAAGCAC AGAAGCTGGT GCAATTCTTA CGGGAATGTG AAGATGTCAT GGACTGGATC
    AATGACAAGG AAGCAATAGT GACCTCAGAA GAGCTTGGAC AGGACTTAGA GCATGTTGAG
    GTTTTGCAGA AGAAATTTGA AGAGTTCCAA ACAGATCTGG CAGCTCATGA GGAGAGAGTG
    AATGAAGTGA ACCAGTTCGC TGGCAAACTT ATCCAGGAAC AGCACCCTGA GGAGGAACTT
    ATAAAGTCCA AACAGGATGA AGTAAATGCA AGCTGGCAGC GTCTTAAGGG GCTTGCCCAA
    CAGAGGCAAG GAAAACTCTT TGGGGCAGCT GAAGTTCAGC GCTTCAACAG GGATGTGGAT
    GAAACTATCA GCTGGATTAA GGAGAAAGGG CAGTTGATGG CCTCAGATGA CTTTGGCAGA
    GACTTGGCCA GCGTGCAAGC TTTACTACGT AAGCATGAAG GCCTGGAAAG AGATCTTGCA
    GCTTTGGAGG ATAAGGTTAA GGCCCTCTGT GCAGAAGCTG ACCGTTTGCA GCAGTCTCAC
    CCTATAAATG CTTCTCAAAT TCAAGTGAAA CGGGAGGAAC TGATTGCCAA CTGGGAGCAG
    ATCCGAACTC TGGCAGCAGA GAGGCATGCT CGCCTTAATG ACTCCTACAG GTTGCAGCGC
    TTTCTTGCAG ATTTCCGGGA CCTCACTAGC TGGGTAACTG AGATGAAGGC TCTGATAAAT
    GCTGATGAGC TTGCCAATGA TGTGGCTGGA GCAGAAGCCC TTCTAGATAG ACATCAGGAA
    CATAAGGGAG AAATTGATGC TCATGAGGAT AGCTTCAGAT CTGCTGATGA GTCTGGGCAG
    GCTTTGCTTG CAGCTGGGCA CTATGCTTCT GATGAAGTTA AAGAAAAGCT GACTATCCTC
    TCAGATGAAA GATCAGCCTT GCTGGAACTA TGGGAGCTTC GCAGGCAACA GTATGAGCAG
    TGCATGGATC TGCAGCTTTT CTACAGAGAT ACTGAACAAG TTGACAACTG GATGAGCAAA
    CAAGAAGCTT TTCTGCTGAA CGAAGACCTT GGTGATTCTC TGGATAGCGT GGAGGCTCTT
    CTAAAGAAGC ATGAAGATTT TGAGAAATCC CTAAGTGCTC AAGAGGAGAA GATCACAGCA
    CTGGATGAGT TTGCTACTAA ACTGATTCAA AATAACCATT ATGCCATGGA TGACGTTGCT
    ACGCGCAGAG ATGCTCTTCT GAGTCGCCGA AATGCTCTTC ATGAAAGAGC CATGAAGCGC
    CGTGCCCAAC TGGCAGACTC TTTCCATCTC CAGCAGTTTT TCAGGGACTC TGATGAACTG
    AAGAGTTGGG TTAATGAAAA AATGAAAACT GCTACCGATG AGGCTTACAA GGATCCATCC
    AACTTGCAAG GTAAAGTTCA GAAGCATCAG GCTTTTGAAG CAGAGCTTTC TGCTAATCAG
    AGTCGTATTG ATGCACTGGA GAAAGCTGGC CAGAAGTTGA TTGATGTCAA TCACTATGCA
    TCTGATGAGG TGGCAGCTCG CATGAATGAA GTCATCAGCT TGTGGAAGAA ACTTCTGGAA
    GCCACCGAGC TCAAAGGTAT AAAGCTGCGT GAAGCCAATC AACAGCAGCA ATTTAATCGC
    AATGTAGAAG ACATTGAGTT GTGGCTGTAT GAAGTGGAGG GTCACTTGGC TTCTGATGAT
    TATGGAAAAG ATCTTACCAG CGTTCAGAAT CTTCAGAAGA AACATGCCCT GCTAGAGGCA
    GATGTTGCTG CCCATCAGGA TCGGATAGAT GGCATTACCA TCCAGGCACG CCAGTTCCAA
    GATGCTGGGC ACTTTGATGC TGACAATATC AAGAAGAAAC AAGAAGCTTT AGTAGCTCGT
    TATGAAGCTC TGAAGGATCC TATGGTGGCT CGCAAGCAGA AACTTGCAGA TTCTCTTCGC
    CTGCAGCAGC TTTTCCGTGA CATCGAGGAT GAAGAGACCT GGATCAGGGA AAAGGAACCT
    ATTGCAGCCT CAACAAACCG AGGCAAGGAC TTAATTGGTG TCCAGAATCT GCTAAAGAAG
    CACCAGGCTT TGCAGGCAGA AATTGCAGGC CATGAGCCTC GCATTAAAGC AGTCACACAA
    AAAGGAAATG CGATGGTGGA AGAGGGACAT TTTGCTGCTG AAGATGTGAA GATCAAATTG
    AATGAACTAA ACCAAAAATG GGACTCTCTG AAAGCCAAGG CATCTCAACG GCGACAGGAT
    CTAGAGGATT CTCTGCAAGC TCAGCAGTAT TTTGCTGATG CTAATGAGGC AGAATCATGG
    ATGAGGGAAA AGGAGCCCAT TGTAGGCAGC ACGGATTATG GAAAGGATGA AGACTCTGCT
    GAGGCTCTCC TGAAGAAGCA TGAAGCTTTG ATGTCTGATC TTTCTGCTTA CGGCAGTAGC
    ATACAGGCAT TAAGGGAACA GGCCCAGTCC TGCAGGCAAC AAGTTGCTCC CACTGATGAT
    GAAACTGGAA AAGAGCTTGT GCTAGCACTC TATGATTACC AAGAGAAGAG TCCTCGGGAG
    GTGACTATGA AGAAAGGAGA TATTCTAACC CTGCTCAACA GCACCAACAA GGACTGGTGG
    AAGGTTGAAG TTAACGATCG TCAGGGCTTT GTACCAGCTG CCTATGTGAA AAAACTAGAT
    CCTGCCCAGT CTGCATCCCG AGAGAATCTT CTAGAAGAGC AAGGCAGCAT AGCATTGCGA
    CAGGAGCAAA TTGACAACCA GACTCTCATT ACTAAGGAAG TCGGCAGTGT ATCTCTGCGT
    ATGAAACAGG TCGAAGAACT GTATCATTCC CTTCTTGAGC TGGGAGAAAA ACGTAAAGGC
    ATGCTAGAAA AAAGCTGCAA GAAGTTTATG CTTTTCCGTG AGGCTAATGA GCTTCAACAG
    TGGATCAATG AGAAGGAAGC TGCGCTCACT AATGAGGAAG TGGGTGCTGA TTTGGAGCAG
    GTGGAGGTGC TACAGAAGAA GTTTGATGAT TTTCAGAAGG ATTTAAAAGC TAATGAGTCA
    CGACTGAAGG ACATAAACAA GGTTGCAAAT GACCTGGAGT CTGAAGGGCT GATGGCAGAG
    GAAGTGCAAG CAGTAGAGCA CCAGGAAGTC TATGGAATGA TGCCCAGAGA TGAAACTGAT
    TCTAAGACAG CCTCTCCTTG GAAGTCTGCA CGTATGATGG TACACACAGT GGCAACGTTT
    AACTCAATCA AGGAACTGAA TGAACGCTGG AGATCCCTGC AGCAGTTGGC AGAGGAGCGA
    AGCCAGCTGT TGGGCAGCGC TCATGAAGTC CAGAGGTTCC ACAGAGATGC TGATGAAACA
    AAAGAATGGA TAGAAGAGAA GAATCAAGCA TTAAATACCG ACAACTATGG ACATGACTTG
    GCCAGTGTGC AGGCTCTGCA GCGCAAGCAT GAAGGCTTTG AGAGAGACTT GGCAGCTCTT
    GGAGACAAGG TGAATTCTCT TGGTGAAACT GCCCAGCGTC TGATCCAGTC ACATCCAGAA
    TCAGCTGAAG ATCTTCAAGA AAAATGTACT GAGTTGAACC AAGCCTGGAG TAGTCTGGGA
    AAACGTGCTG ACCAACGCAA AGAGAAGCTT GGAGATTCTC ATGACCTGCA GCGTTTCCTC
    AGTGATTTTA GAGACCTTAT GTCTTGGATC AATGGAATCC GGGGTCTGGT CTCCTCAGAT
    GAACTTGCAA AAGATGTGAC TGGAGCTGAA GCTCTGTTGG AAAGGCATCA GGAGCACCGT
    ACTGAAATAG ATGCACGGGC TGGCACTTTT CAGGCATTTG AACAGTTTGG ACAGCAGCTT
    CTGGCTCGTG GACACTATGC CAGCCCAGAG ATTAAGGAGA AACTGGATAT TCTAGATCAA
    GAACGGACAG ACCTAGAGAA GGCCTGGGTC CAGCGCAGAA TGATGCTAGA CCAGTGCTTA
    GAACTACAGC TGTTTCATCG GGATTGTGAA CAAGCTGAAA ACTGGATGGC TGCCCGAGAG
    GCGTTCCTAA ATACAGAAGA CAAAGGAGAC TCCTTAGACA GCGTGGAGGC ACTCATCAAG
    AAGCATGAAG ATTTCGATAA AGCAATCAAT GTCCAGGAAG AGAAAATTGC TGTCTTGCAG
    TCTTTTGCTG ACCAACTGAT TGCTGCTGAT CATTATGCAA AAGGAGTCAT TGCTAACAGA
    CGCAATGAGG TTCTGGACAG GTGGCGTCGT CTGAAGGCTC AGATGATTGA GAAGAGATCT
    AAGCTGGGAG AATCTCAGAC CCTCCAACAG TTCAGTCGTG ATGTTGATGA AATAGAAGCT
    TGGATCAGTG AAAAGCTTCA AACTGCAAGT GATGAGTCAT ATAAGGATCC CACAAACATC
    CAGAGCAAGC ACCAGAAGCA CCAAGCCTTT GAAGCTGAGC TCCACGCCAA CGCAGATCGG
    ATTCGTGGAG TCATTGAAAT GGGGAACTCT CTTATTGAAA GAGGAGCGTG TGCTGGCAGC
    GAGGATGCTG TGAAGGCTCG GCTGGCTGCC CTGGCTGACC AATGGGAGTT CCTGGTCCAG
    AAGTCATCAG AGAAGAGTCA GAAACTGAAA GAAGCAAATA AACAGCAGAA TTTCAATACC
    GGCATCAAGG ACTTTGATTT CTGGCTTTCA GAGGTGGAAG CTTTGTTGGC ATCTGAAGAC
    TATGGGAAGG ACTTGGCATC AGTGAACAAC CTTCTGAAGA AGCACCAATT ACTGGAAGCT
    GATATATCTG CTCATGAGGA TCGGCTGAAG GACCTGAACA GCCAGGCTGA CAGTTTGATG
    ACCAGCAGTG CTTTCGATAC CTCCCAAGTA AAGGATAAAC GTGAAACTAT AAATGGGCGC
    TTCCAGAGAA TCAAGAGCAT GGCAGCTGCC CGCCGTGCGA AGCTCAACGA GTCGCACCGC
    TTGCATCAGT TCTTCCGTGA CATGGATGAT GAGGAGTCCT GGATCAAAGA GAAGAAACTG
    TTGGTGAGCT CAGAGGACTA TGGCAGAGAC CTGACTGGTG TGCAGAACCT GAGGAAGAAA
    CATAAGCGCT TGGAAGCAGA ATTAGCTGCC CATGAACCTG CTATCCAGGG TGTTCTAGAC
    ACTGGCAAGA AGCTTTCAGA TGATAACACA ATTGGGAAGG AGGAGATACA GCAAAGACTG
    GCTCAGTTTG TGGACCACTG GAAAGAGTTA AAACAGCTGG CAGCTGCTCG GGGGCAGCGT
    CTGGAGGAGT CCCTGGAGTA CCAACAGTTT GTAGCAAATG TTGAGGAAGA GGAGGCATGG
    ATCAACGAGA AAATGACATT GGTAGCCAGT GAGGATTATG GAGACACACT TGCTGCTATC
    CAGGGCTTGC TGAAGAAGCA TGAAGCATTC GAGACTGACT TTACTGTCCA CAAAGACAGA
    GTGAATGATG TTTGCGCTAA TGGAGAGGAT CTCATTAAAA AGAATAATCA CCATGTGGAG
    AACATTACTG CTAAGATGAA GGGCCTCAAG GGCAAGGTAT CAGATCTGGA GAAAGCCGCA
    GCTCAGAGGA AAGCCAAACT GGATGAGAAC TCTGCCTTCC TCCAGTTCAA CTGGAAAGCA
    GATGTGGTGG AGTCATGGAT AGGTGAGAAG GAAAACAGTC TGAAGACAGA TGATTATGGC
    CGTGACCTCT CCTCTGTGCA AACACTACTC ACCAAACAGG AAACCTTTGA TGCTGGACTT
    CAGGCTTTCC AGCAGGAGGG AATTGCAAAC ATCACTGCTC TGAAAGACCA GCTACTGGCA
    GCCAAACATA TCCAATCAAA GGCCATTGAG GTTCGTCACG CTTCCTTGAT GAAACGCTGG
    AATCAGCTGC TTGCTAATTC TGCAGCCAGG AAAAAGAAAC TCTTGGAGGC TCAGGAGCAC
    TTCAGAAAGG TTGAAGATCT CTTCTTGACT TTTGCCAAGA AGGCCTCTGC CTTCAACAGT
    TGGTTTGAGA ATGCTGAGGA GGACCTGACG GATCCCGTGC GCTGCAATTC CCTGGAAGAA
    ATCAAAGCAC TGCGAGAAGC CCACGATGCG TTCCGTTCCT CACTTAGCTC TGCCCAAGCT
    GACTTCAACC AGCTGGCAGA GCTTGATCGG CAGATCAAGA GCTTCCGTGT AGCCTCCAAC
    CCGTACACTT GGTTTACTAT GGAGGCTCTT GAAGAAACTT GGAGGAATCT GCAGAAAATT
    ATCAAGGAGC GTGAATTGGA GTTGCAGAAG GAGCAGCGGA GGCAGGAAGA AAATGACAAG
    TTGCGCCAGG AGTTTGCTCA GCATGCTAAT GCCTTCCACC AGTGGATTCA GGAGACAAGG
    ACTTACCTGC TAGATGGCAT AGCCTATCGT CGTGTCATTC GTGTCTATCA GTATGAAGTT
    GGGGATGATC TATCTGGAAG GTCCTGCATG GTGGAGGAGT CGGGAACACT GGAATCCCAG
    CTGGAAGCTA CTAAACGCAA GCACCAGGAG ATCCGGGCTA TGAGGAGCCA GCTGAAGAAG
    ATTGAGGACC TTGGAGCAGC CATGGAAGAG GCACTTATCT TGGACAACAA ATACACAGAG
    CACAGCACCG TGGGGCTGGC GCAGCAGTGG GACCAGCTTG ACCAGCTGGG AATGAGAATG
    CAGCACAATC TGGAACAGCA GATTCAAGCT CGAAACACAA CTGGAGTCAC AGAGGAGGCC
    CTGAAGGAGT TCAGCATGAT GTTCAAGCAC TTTGACAAGG ACAAATCTGG ACGTCTTAAT
    CACCAGGAGT TTAAGTCTTG CTTGCGCTCC CTTGGCTATG ACCTGCCCAT GGTTGAGGAA
    GGAGAGCCTG ACCCCGAATT TGAGTCTATT CTTGACACTG TTGATCCCAA CAGGGATGGC
    CACGTCTCGC TGCAGGAGTA CATGGCGTTC ATGATCAGCA GGGAAACAGA GAACGTGAAA
    TCCAGCGAGG AGATTGAGAG TGCTTTCCGT GCCCTCAGCT CGGAGGGGAA GCCTTACGTG
    ACCAAGGAGG AGCTCTACCA GAACCTGACC CGGGAGCAGG CCGACTACTG CATTTCTCAC
    ATGAAGCCCT ACATGGATGG CAAGGGCAGA GAACTTCCTT CTGCCTACGA CTACATAGAA
    TTTACACGTT CACTCTTTGT GAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa43310
    Clone ID Related Accession (Same CDS sequence) XM_015279284.1 , XM_015279284.2
    Accession Version XM_015279284.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7566bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    ATGGATCCAA GTGGGGTAAA AGTGTTGGAA ACAGCAGAAG ATATCCAAGA ACGGCGTCAG 
    CAGGTTTTGG ACCGTTACCA CAGGTTCAAG GAGCTGTCTT CTCTGAGGCG CCAAAAACTC
    GAAGATTCCT ATCGGTTCCA GTTCTTCCAG CGCGATGCAG ATGAGCTGGA AAAATGGATC
    CAAGAGAAAC TCCAGATAGC ATCTGATGAA AATTACAAAG ACCCAAGCAA TTTACAGGGG
    AAGCTGCAGA AGCACCAGGC CTTTGAAGCT GAGGTGCAGG CCAATTCAGG GGCTATCGTT
    AAGCTGGATG AGACTGGAAA TCAGATGATC AATGAAGGCC ATTTTGCATC TGAAACCATA
    AGATCTTTCA CGCCTTACAG GACTGCACAG CGCCCAATAG TAACAGCCAC CCAAGTTGGG
    TCCCACATAC GAACTCGTCT ACAGGAGCTG CACCGACTAT GGGAGTTACT GCTGGAAAAA
    ATGAGAGAGA AGGGAGTGAA GCTGTTGCAA GCACAGAAGC TGGTGCAATT CTTACGGGAA
    TGTGAAGATG TCATGGACTG GATCAATGAC AAGGAAGCAA TAGTGACCTC AGAAGAGCTT
    GGACAGGACT TAGAGCATGT TGAGGTTTTG CAGAAGAAAT TTGAAGAGTT CCAAACAGAT
    CTGGCAGCTC ATGAGGAGAG AGTGAATGAA GTGAACCAGT TCGCTGGCAA ACTTATCCAG
    GAACAGCACC CTGAGGAGGA ACTTATAAAG TCCAAACAGG ATGAAGTAAA TGCAAGCTGG
    CAGCGTCTTA AGGGGCTTGC CCAACAGAGG CAAGGAAAAC TCTTTGGGGC AGCTGAAGTT
    CAGCGCTTCA ACAGGGATGT GGATGAAACT ATCAGCTGGA TTAAGGAGAA AGGGCAGTTG
    ATGGCCTCAG ATGACTTTGG CAGAGACTTG GCCAGCGTGC AAGCTTTACT ACGTAAGCAT
    GAAGGCCTGG AAAGAGATCT TGCAGCTTTG GAGGATAAGG TTAAGGCCCT CTGTGCAGAA
    GCTGACCGTT TGCAGCAGTC TCACCCTATA AATGCTTCTC AAATTCAAGT GAAACGGGAG
    GAACTGATTG CCAACTGGGA GCAGATCCGA ACTCTGGCAG CAGAGAGGCA TGCTCGCCTT
    AATGACTCCT ACAGGTTGCA GCGCTTTCTT GCAGATTTCC GGGACCTCAC TAGCTGGGTA
    ACTGAGATGA AGGCTCTGAT AAATGCTGAT GAGCTTGCCA ATGATGTGGC TGGAGCAGAA
    GCCCTTCTAG ATAGACATCA GGAACATAAG GGAGAAATTG ATGCTCATGA GGATAGCTTC
    AGATCTGCTG ATGAGTCTGG GCAGGCTTTG CTTGCAGCTG GGCACTATGC TTCTGATGAA
    GTTAAAGAAA AGCTGACTAT CCTCTCAGAT GAAAGATCAG CCTTGCTGGA ACTATGGGAG
    CTTCGCAGGC AACAGTATGA GCAGTGCATG GATCTGCAGC TTTTCTACAG AGATACTGAA
    CAAGTTGACA ACTGGATGAG CAAACAAGAA GCTTTTCTGC TGAACGAAGA CCTTGGTGAT
    TCTCTGGATA GCGTGGAGGC TCTTCTAAAG AAGCATGAAG ATTTTGAGAA ATCCCTAAGT
    GCTCAAGAGG AGAAGATCAC AGCACTGGAT GAGTTTGCTA CTAAACTGAT TCAAAATAAC
    CATTATGCCA TGGATGACGT TGCTACGCGC AGAGATGCTC TTCTGAGTCG CCGAAATGCT
    CTTCATGAAA GAGCCATGAA GCGCCGTGCC CAACTGGCAG ACTCTTTCCA TCTCCAGCAG
    TTTTTCAGGG ACTCTGATGA ACTGAAGAGT TGGGTTAATG AAAAAATGAA AACTGCTACC
    GATGAGGCTT ACAAGGATCC ATCCAACTTG CAAGGTAAAG TTCAGAAGCA TCAGGCTTTT
    GAAGCAGAGC TTTCTGCTAA TCAGAGTCGT ATTGATGCAC TGGAGAAAGC TGGCCAGAAG
    TTGATTGATG TCAATCACTA TGCATCTGAT GAGGTGGCAG CTCGCATGAA TGAAGTCATC
    AGCTTGTGGA AGAAACTTCT GGAAGCCACC GAGCTCAAAG GTATAAAGCT GCGTGAAGCC
    AATCAACAGC AGCAATTTAA TCGCAATGTA GAAGACATTG AGTTGTGGCT GTATGAAGTG
    GAGGGTCACT TGGCTTCTGA TGATTATGGA AAAGATCTTA CCAGCGTTCA GAATCTTCAG
    AAGAAACATG CCCTGCTAGA GGCAGATGTT GCTGCCCATC AGGATCGGAT AGATGGCATT
    ACCATCCAGG CACGCCAGTT CCAAGATGCT GGGCACTTTG ATGCTGACAA TATCAAGAAG
    AAACAAGAAG CTTTAGTAGC TCGTTATGAA GCTCTGAAGG ATCCTATGGT GGCTCGCAAG
    CAGAAACTTG CAGATTCTCT TCGCCTGCAG CAGCTTTTCC GTGACATCGA GGATGAAGAG
    ACCTGGATCA GGGAAAAGGA ACCTATTGCA GCCTCAACAA ACCGAGGCAA GGACTTAATT
    GGTGTCCAGA ATCTGCTAAA GAAGCACCAG GCTTTGCAGG CAGAAATTGC AGGCCATGAG
    CCTCGCATTA AAGCAGTCAC ACAAAAAGGA AATGCGATGG TGGAAGAGGG ACATTTTGCT
    GCTGAAGATG TGAAGATCAA ATTGAATGAA CTAAACCAAA AATGGGACTC TCTGAAAGCC
    AAGGCATCTC AACGGCGACA GGATCTAGAG GATTCTCTGC AAGCTCAGCA GTATTTTGCT
    GATGCTAATG AGGCAGAATC ATGGATGAGG GAAAAGGAGC CCATTGTAGG CAGCACGGAT
    TATGGAAAGG ATGAAGACTC TGCTGAGGCT CTCCTGAAGA AGCATGAAGC TTTGATGTCT
    GATCTTTCTG CTTACGGCAG TAGCATACAG GCATTAAGGG AACAGGCCCA GTCCTGCAGG
    CAACAAGTTG CTCCCACTGA TGATGAAACT GGAAAAGAGC TTGTGCTAGC ACTCTATGAT
    TACCAAGAGA AGAGTCCTCG GGAGGTGACT ATGAAGAAAG GAGATATTCT AACCCTGCTC
    AACAGCACCA ACAAGGACTG GTGGAAGGTT GAAGTTAACG ATCGTCAGGG CTTTGTACCA
    GCTGCCTATG TGAAAAAACT AGATCCTGCC CAGTCTGCAT CCCGAGAGAA TCTTCTAGAA
    GAGCAAGGCA GCATAGCATT GCGACAGGAG CAAATTGACA ACCAGACTCT CATTACTAAG
    GAAGTCGGCA GTGTATCTCT GCGTATGAAA CAGGTCGAAG AACTGTATCA TTCCCTTCTT
    GAGCTGGGAG AAAAACGTAA AGGCATGCTA GAAAAAAGCT GCAAGAAGTT TATGCTTTTC
    CGTGAGGCTA ATGAGCTTCA ACAGTGGATC AATGAGAAGG AAGCTGCGCT CACTAATGAG
    GAAGTGGGTG CTGATTTGGA GCAGGTGGAG GTGCTACAGA AGAAGTTTGA TGATTTTCAG
    AAGGATTTAA AAGCTAATGA GTCACGACTG AAGGACATAA ACAAGGTTGC AAATGACCTG
    GAGTCTGAAG GGCTGATGGC AGAGGAAGTG CAAGCAGTAG AGCACCAGGA AGTCTATGGA
    ATGATGCCCA GAGATGAAAC TGATTCTAAG ACAGCCTCTC CTTGGAAGTC TGCACGTATG
    ATGGTACACA CAGTGGCAAC GTTTAACTCA ATCAAGGAAC TGAATGAACG CTGGAGATCC
    CTGCAGCAGT TGGCAGAGGA GCGAAGCCAG CTGTTGGGCA GCGCTCATGA AGTCCAGAGG
    TTCCACAGAG ATGCTGATGA AACAAAAGAA TGGATAGAAG AGAAGAATCA AGCATTAAAT
    ACCGACAACT ATGGACATGA CTTGGCCAGT GTGCAGGCTC TGCAGCGCAA GCATGAAGGC
    TTTGAGAGAG ACTTGGCAGC TCTTGGAGAC AAGGTGAATT CTCTTGGTGA AACTGCCCAG
    CGTCTGATCC AGTCACATCC AGAATCAGCT GAAGATCTTC AAGAAAAATG TACTGAGTTG
    AACCAAGCCT GGAGTAGTCT GGGAAAACGT GCTGACCAAC GCAAAGAGAA GCTTGGAGAT
    TCTCATGACC TGCAGCGTTT CCTCAGTGAT TTTAGAGACC TTATGTCTTG GATCAATGGA
    ATCCGGGGTC TGGTCTCCTC AGATGAACTT GCAAAAGATG TGACTGGAGC TGAAGCTCTG
    TTGGAAAGGC ATCAGGAGCA CCGTACTGAA ATAGATGCAC GGGCTGGCAC TTTTCAGGCA
    TTTGAACAGT TTGGACAGCA GCTTCTGGCT CGTGGACACT ATGCCAGCCC AGAGATTAAG
    GAGAAACTGG ATATTCTAGA TCAAGAACGG ACAGACCTAG AGAAGGCCTG GGTCCAGCGC
    AGAATGATGC TAGACCAGTG CTTAGAACTA CAGCTGTTTC ATCGGGATTG TGAACAAGCT
    GAAAACTGGA TGGCTGCCCG AGAGGCGTTC CTAAATACAG AAGACAAAGG AGACTCCTTA
    GACAGCGTGG AGGCACTCAT CAAGAAGCAT GAAGATTTCG ATAAAGCAAT CAATGTCCAG
    GAAGAGAAAA TTGCTGTCTT GCAGTCTTTT GCTGACCAAC TGATTGCTGC TGATCATTAT
    GCAAAAGGAG TCATTGCTAA CAGACGCAAT GAGGTTCTGG ACAGGTGGCG TCGTCTGAAG
    GCTCAGATGA TTGAGAAGAG ATCTAAGCTG GGAGAATCTC AGACCCTCCA ACAGTTCAGT
    CGTGATGTTG ATGAAATAGA AGCTTGGATC AGTGAAAAGC TTCAAACTGC AAGTGATGAG
    TCATATAAGG ATCCCACAAA CATCCAGCTT TCCAAACTGC TGAGCAAGCA CCAGAAGCAC
    CAAGCCTTTG AAGCTGAGCT CCACGCCAAC GCAGATCGGA TTCGTGGAGT CATTGAAATG
    GGGAACTCTC TTATTGAAAG AGGAGCGTGT GCTGGCAGCG AGGATGCTGT GAAGGCTCGG
    CTGGCTGCCC TGGCTGACCA ATGGGAGTTC CTGGTCCAGA AGTCATCAGA GAAGAGTCAG
    AAACTGAAAG AAGCAAATAA ACAGCAGAAT TTCAATACCG GCATCAAGGA CTTTGATTTC
    TGGCTTTCAG AGGTGGAAGC TTTGTTGGCA TCTGAAGACT ATGGGAAGGA CTTGGCATCA
    GTGAACAACC TTCTGAAGAA GCACCAATTA CTGGAAGCTG ATATATCTGC TCATGAGGAT
    CGGCTGAAGG ACCTGAACAG CCAGGCTGAC AGTTTGATGA CCAGCAGTGC TTTCGATACC
    TCCCAAGTAA AGGATAAACG TGAAACTATA AATGGGCGCT TCCAGAGAAT CAAGAGCATG
    GCAGCTGCCC GCCGTGCGAA GCTCAACGAG TCGCACCGCT TGCATCAGTT CTTCCGTGAC
    ATGGATGATG AGGAGTCCTG GATCAAAGAG AAGAAACTGT TGGTGAGCTC AGAGGACTAT
    GGCAGAGACC TGACTGGTGT GCAGAACCTG AGGAAGAAAC ATAAGCGCTT GGAAGCAGAA
    TTAGCTGCCC ATGAACCTGC TATCCAGGGT GTTCTAGACA CTGGCAAGAA GCTTTCAGAT
    GATAACACAA TTGGGAAGGA GGAGATACAG CAAAGACTGG CTCAGTTTGT GGACCACTGG
    AAAGAGTTAA AACAGCTGGC AGCTGCTCGG GGGCAGCGTC TGGAGGAGTC CCTGGAGTAC
    CAACAGTTTG TAGCAAATGT TGAGGAAGAG GAGGCATGGA TCAACGAGAA AATGACATTG
    GTAGCCAGTG AGGATTATGG AGACACACTT GCTGCTATCC AGGGCTTGCT GAAGAAGCAT
    GAAGCATTCG AGACTGACTT TACTGTCCAC AAAGACAGAG TGAATGATGT TTGCGCTAAT
    GGAGAGGATC TCATTAAAAA GAATAATCAC CATGTGGAGA ACATTACTGC TAAGATGAAG
    GGCCTCAAGG GCAAGGTATC AGATCTGGAG AAAGCCGCAG CTCAGAGGAA AGCCAAACTG
    GATGAGAACT CTGCCTTCCT CCAGTTCAAC TGGAAAGCAG ATGTGGTGGA GTCATGGATA
    GGTGAGAAGG AAAACAGTCT GAAGACAGAT GATTATGGCC GTGACCTCTC CTCTGTGCAA
    ACACTACTCA CCAAACAGGA AACCTTTGAT GCTGGACTTC AGGCTTTCCA GCAGGAGGGA
    ATTGCAAACA TCACTGCTCT GAAAGACCAG CTACTGGCAG CCAAACATAT CCAATCAAAG
    GCCATTGAGG TTCGTCACGC TTCCTTGATG AAACGCTGGA ATCAGCTGCT TGCTAATTCT
    GCAGCCAGGA AAAAGAAACT CTTGGAGGCT CAGGAGCACT TCAGAAAGGT TGAAGATCTC
    TTCTTGACTT TTGCCAAGAA GGCCTCTGCC TTCAACAGTT GGTTTGAGAA TGCTGAGGAG
    GACCTGACGG ATCCCGTGCG CTGCAATTCC CTGGAAGAAA TCAAAGCACT GCGAGAAGCC
    CACGATGCGT TCCGTTCCTC ACTTAGCTCT GCCCAAGCTG ACTTCAACCA GCTGGCAGAG
    CTTGATCGGC AGATCAAGAG CTTCCGTGTA GCCTCCAACC CGTACACTTG GTTTACTATG
    GAGGCTCTTG AAGAAACTTG GAGGAATCTG CAGAAAATTA TCAAGGAGCG TGAATTGGAG
    TTGCAGAAGG AGCAGCGGAG GCAGGAAGAA AATGACAAGT TGCGCCAGGA GTTTGCTCAG
    CATGCTAATG CCTTCCACCA GTGGATTCAG GAGACAAGGA CTTACCTGCT AGATGGCATA
    GCCTATCGTC GTGTCATTCG TGTCTATCAG TATGAAGTTG GGGATGATCT ATCTGGAAGG
    TCCTGCATGG TGGAGGAGTC GGGAACACTG GAATCCCAGC TGGAAGCTAC TAAACGCAAG
    CACCAGGAGA TCCGGGCTAT GAGGAGCCAG CTGAAGAAGA TTGAGGACCT TGGAGCAGCC
    ATGGAAGAGG CACTTATCTT GGACAACAAA TACACAGAGC ACAGCACCGT GGGGCTGGCG
    CAGCAGTGGG ACCAGCTTGA CCAGCTGGGA ATGAGAATGC AGCACAATCT GGAACAGCAG
    ATTCAAGCTC GAAACACAAC TGGAGTCACA GAGGAGGCCC TGAAGGAGTT CAGCATGATG
    TTCAAGCACT TTGACAAGGA CAAATCTGGA CGTCTTAATC ACCAGGAGTT TAAGTCTTGC
    TTGCGCTCCC TTGGCTATGA CCTGCCCATG GTTGAGGAAG GAGAGCCTGA CCCCGAATTT
    GAGTCTATTC TTGACACTGT TGATCCCAAC AGGGATGGCC ACGTCTCGCT GCAGGAGTAC
    ATGGCGTTCA TGATCAGCAG GGAAACAGAG AACGTGAAAT CCAGCGAGGA GATTGAGAGT
    GCTTTCCGTG CCCTCAGCTC GGAGGGGAAG CCTTACGTGA CCAAGGAGGA GCTCTACCAG
    AACCTGACCC GGGAGCAGGC CGACTACTGC ATTTCTCACA TGAAGCCCTA CATGGATGGC
    AAGGGCAGAG AACTTCCTTC TGCCTACGAC TACATAGAAT TTACACGTTC ACTCTTTGTG
    AATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015134770.1
    CDS110..7675
    Translation

    Target ORF information:

    RefSeq Version XM_015279284.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015279284.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    ATGGATCCAA GTGGGGTAAA AGTGTTGGAA ACAGCAGAAG ATATCCAAGA ACGGCGTCAG 
    CAGGTTTTGG ACCGTTACCA CAGGTTCAAG GAGCTGTCTT CTCTGAGGCG CCAAAAACTC
    GAAGATTCCT ATCGGTTCCA GTTCTTCCAG CGCGATGCAG ATGAGCTGGA AAAATGGATC
    CAAGAGAAAC TCCAGATAGC ATCTGATGAA AATTACAAAG ACCCAAGCAA TTTACAGGGG
    AAGCTGCAGA AGCACCAGGC CTTTGAAGCT GAGGTGCAGG CCAATTCAGG GGCTATCGTT
    AAGCTGGATG AGACTGGAAA TCAGATGATC AATGAAGGCC ATTTTGCATC TGAAACCATA
    AGATCTTTCA CGCCTTACAG GACTGCACAG CGCCCAATAG TAACAGCCAC CCAAGTTGGG
    TCCCACATAC GAACTCGTCT ACAGGAGCTG CACCGACTAT GGGAGTTACT GCTGGAAAAA
    ATGAGAGAGA AGGGAGTGAA GCTGTTGCAA GCACAGAAGC TGGTGCAATT CTTACGGGAA
    TGTGAAGATG TCATGGACTG GATCAATGAC AAGGAAGCAA TAGTGACCTC AGAAGAGCTT
    GGACAGGACT TAGAGCATGT TGAGGTTTTG CAGAAGAAAT TTGAAGAGTT CCAAACAGAT
    CTGGCAGCTC ATGAGGAGAG AGTGAATGAA GTGAACCAGT TCGCTGGCAA ACTTATCCAG
    GAACAGCACC CTGAGGAGGA ACTTATAAAG TCCAAACAGG ATGAAGTAAA TGCAAGCTGG
    CAGCGTCTTA AGGGGCTTGC CCAACAGAGG CAAGGAAAAC TCTTTGGGGC AGCTGAAGTT
    CAGCGCTTCA ACAGGGATGT GGATGAAACT ATCAGCTGGA TTAAGGAGAA AGGGCAGTTG
    ATGGCCTCAG ATGACTTTGG CAGAGACTTG GCCAGCGTGC AAGCTTTACT ACGTAAGCAT
    GAAGGCCTGG AAAGAGATCT TGCAGCTTTG GAGGATAAGG TTAAGGCCCT CTGTGCAGAA
    GCTGACCGTT TGCAGCAGTC TCACCCTATA AATGCTTCTC AAATTCAAGT GAAACGGGAG
    GAACTGATTG CCAACTGGGA GCAGATCCGA ACTCTGGCAG CAGAGAGGCA TGCTCGCCTT
    AATGACTCCT ACAGGTTGCA GCGCTTTCTT GCAGATTTCC GGGACCTCAC TAGCTGGGTA
    ACTGAGATGA AGGCTCTGAT AAATGCTGAT GAGCTTGCCA ATGATGTGGC TGGAGCAGAA
    GCCCTTCTAG ATAGACATCA GGAACATAAG GGAGAAATTG ATGCTCATGA GGATAGCTTC
    AGATCTGCTG ATGAGTCTGG GCAGGCTTTG CTTGCAGCTG GGCACTATGC TTCTGATGAA
    GTTAAAGAAA AGCTGACTAT CCTCTCAGAT GAAAGATCAG CCTTGCTGGA ACTATGGGAG
    CTTCGCAGGC AACAGTATGA GCAGTGCATG GATCTGCAGC TTTTCTACAG AGATACTGAA
    CAAGTTGACA ACTGGATGAG CAAACAAGAA GCTTTTCTGC TGAACGAAGA CCTTGGTGAT
    TCTCTGGATA GCGTGGAGGC TCTTCTAAAG AAGCATGAAG ATTTTGAGAA ATCCCTAAGT
    GCTCAAGAGG AGAAGATCAC AGCACTGGAT GAGTTTGCTA CTAAACTGAT TCAAAATAAC
    CATTATGCCA TGGATGACGT TGCTACGCGC AGAGATGCTC TTCTGAGTCG CCGAAATGCT
    CTTCATGAAA GAGCCATGAA GCGCCGTGCC CAACTGGCAG ACTCTTTCCA TCTCCAGCAG
    TTTTTCAGGG ACTCTGATGA ACTGAAGAGT TGGGTTAATG AAAAAATGAA AACTGCTACC
    GATGAGGCTT ACAAGGATCC ATCCAACTTG CAAGGTAAAG TTCAGAAGCA TCAGGCTTTT
    GAAGCAGAGC TTTCTGCTAA TCAGAGTCGT ATTGATGCAC TGGAGAAAGC TGGCCAGAAG
    TTGATTGATG TCAATCACTA TGCATCTGAT GAGGTGGCAG CTCGCATGAA TGAAGTCATC
    AGCTTGTGGA AGAAACTTCT GGAAGCCACC GAGCTCAAAG GTATAAAGCT GCGTGAAGCC
    AATCAACAGC AGCAATTTAA TCGCAATGTA GAAGACATTG AGTTGTGGCT GTATGAAGTG
    GAGGGTCACT TGGCTTCTGA TGATTATGGA AAAGATCTTA CCAGCGTTCA GAATCTTCAG
    AAGAAACATG CCCTGCTAGA GGCAGATGTT GCTGCCCATC AGGATCGGAT AGATGGCATT
    ACCATCCAGG CACGCCAGTT CCAAGATGCT GGGCACTTTG ATGCTGACAA TATCAAGAAG
    AAACAAGAAG CTTTAGTAGC TCGTTATGAA GCTCTGAAGG ATCCTATGGT GGCTCGCAAG
    CAGAAACTTG CAGATTCTCT TCGCCTGCAG CAGCTTTTCC GTGACATCGA GGATGAAGAG
    ACCTGGATCA GGGAAAAGGA ACCTATTGCA GCCTCAACAA ACCGAGGCAA GGACTTAATT
    GGTGTCCAGA ATCTGCTAAA GAAGCACCAG GCTTTGCAGG CAGAAATTGC AGGCCATGAG
    CCTCGCATTA AAGCAGTCAC ACAAAAAGGA AATGCGATGG TGGAAGAGGG ACATTTTGCT
    GCTGAAGATG TGAAGATCAA ATTGAATGAA CTAAACCAAA AATGGGACTC TCTGAAAGCC
    AAGGCATCTC AACGGCGACA GGATCTAGAG GATTCTCTGC AAGCTCAGCA GTATTTTGCT
    GATGCTAATG AGGCAGAATC ATGGATGAGG GAAAAGGAGC CCATTGTAGG CAGCACGGAT
    TATGGAAAGG ATGAAGACTC TGCTGAGGCT CTCCTGAAGA AGCATGAAGC TTTGATGTCT
    GATCTTTCTG CTTACGGCAG TAGCATACAG GCATTAAGGG AACAGGCCCA GTCCTGCAGG
    CAACAAGTTG CTCCCACTGA TGATGAAACT GGAAAAGAGC TTGTGCTAGC ACTCTATGAT
    TACCAAGAGA AGAGTCCTCG GGAGGTGACT ATGAAGAAAG GAGATATTCT AACCCTGCTC
    AACAGCACCA ACAAGGACTG GTGGAAGGTT GAAGTTAACG ATCGTCAGGG CTTTGTACCA
    GCTGCCTATG TGAAAAAACT AGATCCTGCC CAGTCTGCAT CCCGAGAGAA TCTTCTAGAA
    GAGCAAGGCA GCATAGCATT GCGACAGGAG CAAATTGACA ACCAGACTCT CATTACTAAG
    GAAGTCGGCA GTGTATCTCT GCGTATGAAA CAGGTCGAAG AACTGTATCA TTCCCTTCTT
    GAGCTGGGAG AAAAACGTAA AGGCATGCTA GAAAAAAGCT GCAAGAAGTT TATGCTTTTC
    CGTGAGGCTA ATGAGCTTCA ACAGTGGATC AATGAGAAGG AAGCTGCGCT CACTAATGAG
    GAAGTGGGTG CTGATTTGGA GCAGGTGGAG GTGCTACAGA AGAAGTTTGA TGATTTTCAG
    AAGGATTTAA AAGCTAATGA GTCACGACTG AAGGACATAA ACAAGGTTGC AAATGACCTG
    GAGTCTGAAG GGCTGATGGC AGAGGAAGTG CAAGCAGTAG AGCACCAGGA AGTCTATGGA
    ATGATGCCCA GAGATGAAAC TGATTCTAAG ACAGCCTCTC CTTGGAAGTC TGCACGTATG
    ATGGTACACA CAGTGGCAAC GTTTAACTCA ATCAAGGAAC TGAATGAACG CTGGAGATCC
    CTGCAGCAGT TGGCAGAGGA GCGAAGCCAG CTGTTGGGCA GCGCTCATGA AGTCCAGAGG
    TTCCACAGAG ATGCTGATGA AACAAAAGAA TGGATAGAAG AGAAGAATCA AGCATTAAAT
    ACCGACAACT ATGGACATGA CTTGGCCAGT GTGCAGGCTC TGCAGCGCAA GCATGAAGGC
    TTTGAGAGAG ACTTGGCAGC TCTTGGAGAC AAGGTGAATT CTCTTGGTGA AACTGCCCAG
    CGTCTGATCC AGTCACATCC AGAATCAGCT GAAGATCTTC AAGAAAAATG TACTGAGTTG
    AACCAAGCCT GGAGTAGTCT GGGAAAACGT GCTGACCAAC GCAAAGAGAA GCTTGGAGAT
    TCTCATGACC TGCAGCGTTT CCTCAGTGAT TTTAGAGACC TTATGTCTTG GATCAATGGA
    ATCCGGGGTC TGGTCTCCTC AGATGAACTT GCAAAAGATG TGACTGGAGC TGAAGCTCTG
    TTGGAAAGGC ATCAGGAGCA CCGTACTGAA ATAGATGCAC GGGCTGGCAC TTTTCAGGCA
    TTTGAACAGT TTGGACAGCA GCTTCTGGCT CGTGGACACT ATGCCAGCCC AGAGATTAAG
    GAGAAACTGG ATATTCTAGA TCAAGAACGG ACAGACCTAG AGAAGGCCTG GGTCCAGCGC
    AGAATGATGC TAGACCAGTG CTTAGAACTA CAGCTGTTTC ATCGGGATTG TGAACAAGCT
    GAAAACTGGA TGGCTGCCCG AGAGGCGTTC CTAAATACAG AAGACAAAGG AGACTCCTTA
    GACAGCGTGG AGGCACTCAT CAAGAAGCAT GAAGATTTCG ATAAAGCAAT CAATGTCCAG
    GAAGAGAAAA TTGCTGTCTT GCAGTCTTTT GCTGACCAAC TGATTGCTGC TGATCATTAT
    GCAAAAGGAG TCATTGCTAA CAGACGCAAT GAGGTTCTGG ACAGGTGGCG TCGTCTGAAG
    GCTCAGATGA TTGAGAAGAG ATCTAAGCTG GGAGAATCTC AGACCCTCCA ACAGTTCAGT
    CGTGATGTTG ATGAAATAGA AGCTTGGATC AGTGAAAAGC TTCAAACTGC AAGTGATGAG
    TCATATAAGG ATCCCACAAA CATCCAGCTT TCCAAACTGC TGAGCAAGCA CCAGAAGCAC
    CAAGCCTTTG AAGCTGAGCT CCACGCCAAC GCAGATCGGA TTCGTGGAGT CATTGAAATG
    GGGAACTCTC TTATTGAAAG AGGAGCGTGT GCTGGCAGCG AGGATGCTGT GAAGGCTCGG
    CTGGCTGCCC TGGCTGACCA ATGGGAGTTC CTGGTCCAGA AGTCATCAGA GAAGAGTCAG
    AAACTGAAAG AAGCAAATAA ACAGCAGAAT TTCAATACCG GCATCAAGGA CTTTGATTTC
    TGGCTTTCAG AGGTGGAAGC TTTGTTGGCA TCTGAAGACT ATGGGAAGGA CTTGGCATCA
    GTGAACAACC TTCTGAAGAA GCACCAATTA CTGGAAGCTG ATATATCTGC TCATGAGGAT
    CGGCTGAAGG ACCTGAACAG CCAGGCTGAC AGTTTGATGA CCAGCAGTGC TTTCGATACC
    TCCCAAGTAA AGGATAAACG TGAAACTATA AATGGGCGCT TCCAGAGAAT CAAGAGCATG
    GCAGCTGCCC GCCGTGCGAA GCTCAACGAG TCGCACCGCT TGCATCAGTT CTTCCGTGAC
    ATGGATGATG AGGAGTCCTG GATCAAAGAG AAGAAACTGT TGGTGAGCTC AGAGGACTAT
    GGCAGAGACC TGACTGGTGT GCAGAACCTG AGGAAGAAAC ATAAGCGCTT GGAAGCAGAA
    TTAGCTGCCC ATGAACCTGC TATCCAGGGT GTTCTAGACA CTGGCAAGAA GCTTTCAGAT
    GATAACACAA TTGGGAAGGA GGAGATACAG CAAAGACTGG CTCAGTTTGT GGACCACTGG
    AAAGAGTTAA AACAGCTGGC AGCTGCTCGG GGGCAGCGTC TGGAGGAGTC CCTGGAGTAC
    CAACAGTTTG TAGCAAATGT TGAGGAAGAG GAGGCATGGA TCAACGAGAA AATGACATTG
    GTAGCCAGTG AGGATTATGG AGACACACTT GCTGCTATCC AGGGCTTGCT GAAGAAGCAT
    GAAGCATTCG AGACTGACTT TACTGTCCAC AAAGACAGAG TGAATGATGT TTGCGCTAAT
    GGAGAGGATC TCATTAAAAA GAATAATCAC CATGTGGAGA ACATTACTGC TAAGATGAAG
    GGCCTCAAGG GCAAGGTATC AGATCTGGAG AAAGCCGCAG CTCAGAGGAA AGCCAAACTG
    GATGAGAACT CTGCCTTCCT CCAGTTCAAC TGGAAAGCAG ATGTGGTGGA GTCATGGATA
    GGTGAGAAGG AAAACAGTCT GAAGACAGAT GATTATGGCC GTGACCTCTC CTCTGTGCAA
    ACACTACTCA CCAAACAGGA AACCTTTGAT GCTGGACTTC AGGCTTTCCA GCAGGAGGGA
    ATTGCAAACA TCACTGCTCT GAAAGACCAG CTACTGGCAG CCAAACATAT CCAATCAAAG
    GCCATTGAGG TTCGTCACGC TTCCTTGATG AAACGCTGGA ATCAGCTGCT TGCTAATTCT
    GCAGCCAGGA AAAAGAAACT CTTGGAGGCT CAGGAGCACT TCAGAAAGGT TGAAGATCTC
    TTCTTGACTT TTGCCAAGAA GGCCTCTGCC TTCAACAGTT GGTTTGAGAA TGCTGAGGAG
    GACCTGACGG ATCCCGTGCG CTGCAATTCC CTGGAAGAAA TCAAAGCACT GCGAGAAGCC
    CACGATGCGT TCCGTTCCTC ACTTAGCTCT GCCCAAGCTG ACTTCAACCA GCTGGCAGAG
    CTTGATCGGC AGATCAAGAG CTTCCGTGTA GCCTCCAACC CGTACACTTG GTTTACTATG
    GAGGCTCTTG AAGAAACTTG GAGGAATCTG CAGAAAATTA TCAAGGAGCG TGAATTGGAG
    TTGCAGAAGG AGCAGCGGAG GCAGGAAGAA AATGACAAGT TGCGCCAGGA GTTTGCTCAG
    CATGCTAATG CCTTCCACCA GTGGATTCAG GAGACAAGGA CTTACCTGCT AGATGGCATA
    GCCTATCGTC GTGTCATTCG TGTCTATCAG TATGAAGTTG GGGATGATCT ATCTGGAAGG
    TCCTGCATGG TGGAGGAGTC GGGAACACTG GAATCCCAGC TGGAAGCTAC TAAACGCAAG
    CACCAGGAGA TCCGGGCTAT GAGGAGCCAG CTGAAGAAGA TTGAGGACCT TGGAGCAGCC
    ATGGAAGAGG CACTTATCTT GGACAACAAA TACACAGAGC ACAGCACCGT GGGGCTGGCG
    CAGCAGTGGG ACCAGCTTGA CCAGCTGGGA ATGAGAATGC AGCACAATCT GGAACAGCAG
    ATTCAAGCTC GAAACACAAC TGGAGTCACA GAGGAGGCCC TGAAGGAGTT CAGCATGATG
    TTCAAGCACT TTGACAAGGA CAAATCTGGA CGTCTTAATC ACCAGGAGTT TAAGTCTTGC
    TTGCGCTCCC TTGGCTATGA CCTGCCCATG GTTGAGGAAG GAGAGCCTGA CCCCGAATTT
    GAGTCTATTC TTGACACTGT TGATCCCAAC AGGGATGGCC ACGTCTCGCT GCAGGAGTAC
    ATGGCGTTCA TGATCAGCAG GGAAACAGAG AACGTGAAAT CCAGCGAGGA GATTGAGAGT
    GCTTTCCGTG CCCTCAGCTC GGAGGGGAAG CCTTACGTGA CCAAGGAGGA GCTCTACCAG
    AACCTGACCC GGGAGCAGGC CGACTACTGC ATTTCTCACA TGAAGCCCTA CATGGATGGC
    AAGGGCAGAG AACTTCCTTC TGCCTACGAC TACATAGAAT TTACACGTTC ACTCTTTGTG
    AATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa43310
    Clone ID Related Accession (Same CDS sequence) XM_015279284.1 , XM_015279284.2
    Accession Version XM_015279284.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7566bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015279284.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    ATGGATCCAA GTGGGGTAAA AGTGTTGGAA ACAGCAGAAG ATATCCAAGA ACGGCGTCAG 
    CAGGTTTTGG ACCGTTACCA CAGGTTCAAG GAGCTGTCTT CTCTGAGGCG CCAAAAACTC
    GAAGATTCCT ATCGGTTCCA GTTCTTCCAG CGCGATGCAG ATGAGCTGGA AAAATGGATC
    CAAGAGAAAC TCCAGATAGC ATCTGATGAA AATTACAAAG ACCCAAGCAA TTTACAGGGG
    AAGCTGCAGA AGCACCAGGC CTTTGAAGCT GAGGTGCAGG CCAATTCAGG GGCTATCGTT
    AAGCTGGATG AGACTGGAAA TCAGATGATC AATGAAGGCC ATTTTGCATC TGAAACCATA
    AGATCTTTCA CGCCTTACAG GACTGCACAG CGCCCAATAG TAACAGCCAC CCAAGTTGGG
    TCCCACATAC GAACTCGTCT ACAGGAGCTG CACCGACTAT GGGAGTTACT GCTGGAAAAA
    ATGAGAGAGA AGGGAGTGAA GCTGTTGCAA GCACAGAAGC TGGTGCAATT CTTACGGGAA
    TGTGAAGATG TCATGGACTG GATCAATGAC AAGGAAGCAA TAGTGACCTC AGAAGAGCTT
    GGACAGGACT TAGAGCATGT TGAGGTTTTG CAGAAGAAAT TTGAAGAGTT CCAAACAGAT
    CTGGCAGCTC ATGAGGAGAG AGTGAATGAA GTGAACCAGT TCGCTGGCAA ACTTATCCAG
    GAACAGCACC CTGAGGAGGA ACTTATAAAG TCCAAACAGG ATGAAGTAAA TGCAAGCTGG
    CAGCGTCTTA AGGGGCTTGC CCAACAGAGG CAAGGAAAAC TCTTTGGGGC AGCTGAAGTT
    CAGCGCTTCA ACAGGGATGT GGATGAAACT ATCAGCTGGA TTAAGGAGAA AGGGCAGTTG
    ATGGCCTCAG ATGACTTTGG CAGAGACTTG GCCAGCGTGC AAGCTTTACT ACGTAAGCAT
    GAAGGCCTGG AAAGAGATCT TGCAGCTTTG GAGGATAAGG TTAAGGCCCT CTGTGCAGAA
    GCTGACCGTT TGCAGCAGTC TCACCCTATA AATGCTTCTC AAATTCAAGT GAAACGGGAG
    GAACTGATTG CCAACTGGGA GCAGATCCGA ACTCTGGCAG CAGAGAGGCA TGCTCGCCTT
    AATGACTCCT ACAGGTTGCA GCGCTTTCTT GCAGATTTCC GGGACCTCAC TAGCTGGGTA
    ACTGAGATGA AGGCTCTGAT AAATGCTGAT GAGCTTGCCA ATGATGTGGC TGGAGCAGAA
    GCCCTTCTAG ATAGACATCA GGAACATAAG GGAGAAATTG ATGCTCATGA GGATAGCTTC
    AGATCTGCTG ATGAGTCTGG GCAGGCTTTG CTTGCAGCTG GGCACTATGC TTCTGATGAA
    GTTAAAGAAA AGCTGACTAT CCTCTCAGAT GAAAGATCAG CCTTGCTGGA ACTATGGGAG
    CTTCGCAGGC AACAGTATGA GCAGTGCATG GATCTGCAGC TTTTCTACAG AGATACTGAA
    CAAGTTGACA ACTGGATGAG CAAACAAGAA GCTTTTCTGC TGAACGAAGA CCTTGGTGAT
    TCTCTGGATA GCGTGGAGGC TCTTCTAAAG AAGCATGAAG ATTTTGAGAA ATCCCTAAGT
    GCTCAAGAGG AGAAGATCAC AGCACTGGAT GAGTTTGCTA CTAAACTGAT TCAAAATAAC
    CATTATGCCA TGGATGACGT TGCTACGCGC AGAGATGCTC TTCTGAGTCG CCGAAATGCT
    CTTCATGAAA GAGCCATGAA GCGCCGTGCC CAACTGGCAG ACTCTTTCCA TCTCCAGCAG
    TTTTTCAGGG ACTCTGATGA ACTGAAGAGT TGGGTTAATG AAAAAATGAA AACTGCTACC
    GATGAGGCTT ACAAGGATCC ATCCAACTTG CAAGGTAAAG TTCAGAAGCA TCAGGCTTTT
    GAAGCAGAGC TTTCTGCTAA TCAGAGTCGT ATTGATGCAC TGGAGAAAGC TGGCCAGAAG
    TTGATTGATG TCAATCACTA TGCATCTGAT GAGGTGGCAG CTCGCATGAA TGAAGTCATC
    AGCTTGTGGA AGAAACTTCT GGAAGCCACC GAGCTCAAAG GTATAAAGCT GCGTGAAGCC
    AATCAACAGC AGCAATTTAA TCGCAATGTA GAAGACATTG AGTTGTGGCT GTATGAAGTG
    GAGGGTCACT TGGCTTCTGA TGATTATGGA AAAGATCTTA CCAGCGTTCA GAATCTTCAG
    AAGAAACATG CCCTGCTAGA GGCAGATGTT GCTGCCCATC AGGATCGGAT AGATGGCATT
    ACCATCCAGG CACGCCAGTT CCAAGATGCT GGGCACTTTG ATGCTGACAA TATCAAGAAG
    AAACAAGAAG CTTTAGTAGC TCGTTATGAA GCTCTGAAGG ATCCTATGGT GGCTCGCAAG
    CAGAAACTTG CAGATTCTCT TCGCCTGCAG CAGCTTTTCC GTGACATCGA GGATGAAGAG
    ACCTGGATCA GGGAAAAGGA ACCTATTGCA GCCTCAACAA ACCGAGGCAA GGACTTAATT
    GGTGTCCAGA ATCTGCTAAA GAAGCACCAG GCTTTGCAGG CAGAAATTGC AGGCCATGAG
    CCTCGCATTA AAGCAGTCAC ACAAAAAGGA AATGCGATGG TGGAAGAGGG ACATTTTGCT
    GCTGAAGATG TGAAGATCAA ATTGAATGAA CTAAACCAAA AATGGGACTC TCTGAAAGCC
    AAGGCATCTC AACGGCGACA GGATCTAGAG GATTCTCTGC AAGCTCAGCA GTATTTTGCT
    GATGCTAATG AGGCAGAATC ATGGATGAGG GAAAAGGAGC CCATTGTAGG CAGCACGGAT
    TATGGAAAGG ATGAAGACTC TGCTGAGGCT CTCCTGAAGA AGCATGAAGC TTTGATGTCT
    GATCTTTCTG CTTACGGCAG TAGCATACAG GCATTAAGGG AACAGGCCCA GTCCTGCAGG
    CAACAAGTTG CTCCCACTGA TGATGAAACT GGAAAAGAGC TTGTGCTAGC ACTCTATGAT
    TACCAAGAGA AGAGTCCTCG GGAGGTGACT ATGAAGAAAG GAGATATTCT AACCCTGCTC
    AACAGCACCA ACAAGGACTG GTGGAAGGTT GAAGTTAACG ATCGTCAGGG CTTTGTACCA
    GCTGCCTATG TGAAAAAACT AGATCCTGCC CAGTCTGCAT CCCGAGAGAA TCTTCTAGAA
    GAGCAAGGCA GCATAGCATT GCGACAGGAG CAAATTGACA ACCAGACTCT CATTACTAAG
    GAAGTCGGCA GTGTATCTCT GCGTATGAAA CAGGTCGAAG AACTGTATCA TTCCCTTCTT
    GAGCTGGGAG AAAAACGTAA AGGCATGCTA GAAAAAAGCT GCAAGAAGTT TATGCTTTTC
    CGTGAGGCTA ATGAGCTTCA ACAGTGGATC AATGAGAAGG AAGCTGCGCT CACTAATGAG
    GAAGTGGGTG CTGATTTGGA GCAGGTGGAG GTGCTACAGA AGAAGTTTGA TGATTTTCAG
    AAGGATTTAA AAGCTAATGA GTCACGACTG AAGGACATAA ACAAGGTTGC AAATGACCTG
    GAGTCTGAAG GGCTGATGGC AGAGGAAGTG CAAGCAGTAG AGCACCAGGA AGTCTATGGA
    ATGATGCCCA GAGATGAAAC TGATTCTAAG ACAGCCTCTC CTTGGAAGTC TGCACGTATG
    ATGGTACACA CAGTGGCAAC GTTTAACTCA ATCAAGGAAC TGAATGAACG CTGGAGATCC
    CTGCAGCAGT TGGCAGAGGA GCGAAGCCAG CTGTTGGGCA GCGCTCATGA AGTCCAGAGG
    TTCCACAGAG ATGCTGATGA AACAAAAGAA TGGATAGAAG AGAAGAATCA AGCATTAAAT
    ACCGACAACT ATGGACATGA CTTGGCCAGT GTGCAGGCTC TGCAGCGCAA GCATGAAGGC
    TTTGAGAGAG ACTTGGCAGC TCTTGGAGAC AAGGTGAATT CTCTTGGTGA AACTGCCCAG
    CGTCTGATCC AGTCACATCC AGAATCAGCT GAAGATCTTC AAGAAAAATG TACTGAGTTG
    AACCAAGCCT GGAGTAGTCT GGGAAAACGT GCTGACCAAC GCAAAGAGAA GCTTGGAGAT
    TCTCATGACC TGCAGCGTTT CCTCAGTGAT TTTAGAGACC TTATGTCTTG GATCAATGGA
    ATCCGGGGTC TGGTCTCCTC AGATGAACTT GCAAAAGATG TGACTGGAGC TGAAGCTCTG
    TTGGAAAGGC ATCAGGAGCA CCGTACTGAA ATAGATGCAC GGGCTGGCAC TTTTCAGGCA
    TTTGAACAGT TTGGACAGCA GCTTCTGGCT CGTGGACACT ATGCCAGCCC AGAGATTAAG
    GAGAAACTGG ATATTCTAGA TCAAGAACGG ACAGACCTAG AGAAGGCCTG GGTCCAGCGC
    AGAATGATGC TAGACCAGTG CTTAGAACTA CAGCTGTTTC ATCGGGATTG TGAACAAGCT
    GAAAACTGGA TGGCTGCCCG AGAGGCGTTC CTAAATACAG AAGACAAAGG AGACTCCTTA
    GACAGCGTGG AGGCACTCAT CAAGAAGCAT GAAGATTTCG ATAAAGCAAT CAATGTCCAG
    GAAGAGAAAA TTGCTGTCTT GCAGTCTTTT GCTGACCAAC TGATTGCTGC TGATCATTAT
    GCAAAAGGAG TCATTGCTAA CAGACGCAAT GAGGTTCTGG ACAGGTGGCG TCGTCTGAAG
    GCTCAGATGA TTGAGAAGAG ATCTAAGCTG GGAGAATCTC AGACCCTCCA ACAGTTCAGT
    CGTGATGTTG ATGAAATAGA AGCTTGGATC AGTGAAAAGC TTCAAACTGC AAGTGATGAG
    TCATATAAGG ATCCCACAAA CATCCAGCTT TCCAAACTGC TGAGCAAGCA CCAGAAGCAC
    CAAGCCTTTG AAGCTGAGCT CCACGCCAAC GCAGATCGGA TTCGTGGAGT CATTGAAATG
    GGGAACTCTC TTATTGAAAG AGGAGCGTGT GCTGGCAGCG AGGATGCTGT GAAGGCTCGG
    CTGGCTGCCC TGGCTGACCA ATGGGAGTTC CTGGTCCAGA AGTCATCAGA GAAGAGTCAG
    AAACTGAAAG AAGCAAATAA ACAGCAGAAT TTCAATACCG GCATCAAGGA CTTTGATTTC
    TGGCTTTCAG AGGTGGAAGC TTTGTTGGCA TCTGAAGACT ATGGGAAGGA CTTGGCATCA
    GTGAACAACC TTCTGAAGAA GCACCAATTA CTGGAAGCTG ATATATCTGC TCATGAGGAT
    CGGCTGAAGG ACCTGAACAG CCAGGCTGAC AGTTTGATGA CCAGCAGTGC TTTCGATACC
    TCCCAAGTAA AGGATAAACG TGAAACTATA AATGGGCGCT TCCAGAGAAT CAAGAGCATG
    GCAGCTGCCC GCCGTGCGAA GCTCAACGAG TCGCACCGCT TGCATCAGTT CTTCCGTGAC
    ATGGATGATG AGGAGTCCTG GATCAAAGAG AAGAAACTGT TGGTGAGCTC AGAGGACTAT
    GGCAGAGACC TGACTGGTGT GCAGAACCTG AGGAAGAAAC ATAAGCGCTT GGAAGCAGAA
    TTAGCTGCCC ATGAACCTGC TATCCAGGGT GTTCTAGACA CTGGCAAGAA GCTTTCAGAT
    GATAACACAA TTGGGAAGGA GGAGATACAG CAAAGACTGG CTCAGTTTGT GGACCACTGG
    AAAGAGTTAA AACAGCTGGC AGCTGCTCGG GGGCAGCGTC TGGAGGAGTC CCTGGAGTAC
    CAACAGTTTG TAGCAAATGT TGAGGAAGAG GAGGCATGGA TCAACGAGAA AATGACATTG
    GTAGCCAGTG AGGATTATGG AGACACACTT GCTGCTATCC AGGGCTTGCT GAAGAAGCAT
    GAAGCATTCG AGACTGACTT TACTGTCCAC AAAGACAGAG TGAATGATGT TTGCGCTAAT
    GGAGAGGATC TCATTAAAAA GAATAATCAC CATGTGGAGA ACATTACTGC TAAGATGAAG
    GGCCTCAAGG GCAAGGTATC AGATCTGGAG AAAGCCGCAG CTCAGAGGAA AGCCAAACTG
    GATGAGAACT CTGCCTTCCT CCAGTTCAAC TGGAAAGCAG ATGTGGTGGA GTCATGGATA
    GGTGAGAAGG AAAACAGTCT GAAGACAGAT GATTATGGCC GTGACCTCTC CTCTGTGCAA
    ACACTACTCA CCAAACAGGA AACCTTTGAT GCTGGACTTC AGGCTTTCCA GCAGGAGGGA
    ATTGCAAACA TCACTGCTCT GAAAGACCAG CTACTGGCAG CCAAACATAT CCAATCAAAG
    GCCATTGAGG TTCGTCACGC TTCCTTGATG AAACGCTGGA ATCAGCTGCT TGCTAATTCT
    GCAGCCAGGA AAAAGAAACT CTTGGAGGCT CAGGAGCACT TCAGAAAGGT TGAAGATCTC
    TTCTTGACTT TTGCCAAGAA GGCCTCTGCC TTCAACAGTT GGTTTGAGAA TGCTGAGGAG
    GACCTGACGG ATCCCGTGCG CTGCAATTCC CTGGAAGAAA TCAAAGCACT GCGAGAAGCC
    CACGATGCGT TCCGTTCCTC ACTTAGCTCT GCCCAAGCTG ACTTCAACCA GCTGGCAGAG
    CTTGATCGGC AGATCAAGAG CTTCCGTGTA GCCTCCAACC CGTACACTTG GTTTACTATG
    GAGGCTCTTG AAGAAACTTG GAGGAATCTG CAGAAAATTA TCAAGGAGCG TGAATTGGAG
    TTGCAGAAGG AGCAGCGGAG GCAGGAAGAA AATGACAAGT TGCGCCAGGA GTTTGCTCAG
    CATGCTAATG CCTTCCACCA GTGGATTCAG GAGACAAGGA CTTACCTGCT AGATGGCATA
    GCCTATCGTC GTGTCATTCG TGTCTATCAG TATGAAGTTG GGGATGATCT ATCTGGAAGG
    TCCTGCATGG TGGAGGAGTC GGGAACACTG GAATCCCAGC TGGAAGCTAC TAAACGCAAG
    CACCAGGAGA TCCGGGCTAT GAGGAGCCAG CTGAAGAAGA TTGAGGACCT TGGAGCAGCC
    ATGGAAGAGG CACTTATCTT GGACAACAAA TACACAGAGC ACAGCACCGT GGGGCTGGCG
    CAGCAGTGGG ACCAGCTTGA CCAGCTGGGA ATGAGAATGC AGCACAATCT GGAACAGCAG
    ATTCAAGCTC GAAACACAAC TGGAGTCACA GAGGAGGCCC TGAAGGAGTT CAGCATGATG
    TTCAAGCACT TTGACAAGGA CAAATCTGGA CGTCTTAATC ACCAGGAGTT TAAGTCTTGC
    TTGCGCTCCC TTGGCTATGA CCTGCCCATG GTTGAGGAAG GAGAGCCTGA CCCCGAATTT
    GAGTCTATTC TTGACACTGT TGATCCCAAC AGGGATGGCC ACGTCTCGCT GCAGGAGTAC
    ATGGCGTTCA TGATCAGCAG GGAAACAGAG AACGTGAAAT CCAGCGAGGA GATTGAGAGT
    GCTTTCCGTG CCCTCAGCTC GGAGGGGAAG CCTTACGTGA CCAAGGAGGA GCTCTACCAG
    AACCTGACCC GGGAGCAGGC CGACTACTGC ATTTCTCACA TGAAGCCCTA CATGGATGGC
    AAGGGCAGAG AACTTCCTTC TGCCTACGAC TACATAGAAT TTACACGTTC ACTCTTTGTG
    AATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015134770.1
    CDS159..7724
    Translation

    Target ORF information:

    RefSeq Version XM_015279284.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015279284.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    ATGGATCCAA GTGGGGTAAA AGTGTTGGAA ACAGCAGAAG ATATCCAAGA ACGGCGTCAG 
    CAGGTTTTGG ACCGTTACCA CAGGTTCAAG GAGCTGTCTT CTCTGAGGCG CCAAAAACTC
    GAAGATTCCT ATCGGTTCCA GTTCTTCCAG CGCGATGCAG ATGAGCTGGA AAAATGGATC
    CAAGAGAAAC TCCAGATAGC ATCTGATGAA AATTACAAAG ACCCAAGCAA TTTACAGGGG
    AAGCTGCAGA AGCACCAGGC CTTTGAAGCT GAGGTGCAGG CCAATTCAGG GGCTATCGTT
    AAGCTGGATG AGACTGGAAA TCAGATGATC AATGAAGGCC ATTTTGCATC TGAAACCATA
    AGATCTTTCA CGCCTTACAG GACTGCACAG CGCCCAATAG TAACAGCCAC CCAAGTTGGG
    TCCCACATAC GAACTCGTCT ACAGGAGCTG CACCGACTAT GGGAGTTACT GCTGGAAAAA
    ATGAGAGAGA AGGGAGTGAA GCTGTTGCAA GCACAGAAGC TGGTGCAATT CTTACGGGAA
    TGTGAAGATG TCATGGACTG GATCAATGAC AAGGAAGCAA TAGTGACCTC AGAAGAGCTT
    GGACAGGACT TAGAGCATGT TGAGGTTTTG CAGAAGAAAT TTGAAGAGTT CCAAACAGAT
    CTGGCAGCTC ATGAGGAGAG AGTGAATGAA GTGAACCAGT TCGCTGGCAA ACTTATCCAG
    GAACAGCACC CTGAGGAGGA ACTTATAAAG TCCAAACAGG ATGAAGTAAA TGCAAGCTGG
    CAGCGTCTTA AGGGGCTTGC CCAACAGAGG CAAGGAAAAC TCTTTGGGGC AGCTGAAGTT
    CAGCGCTTCA ACAGGGATGT GGATGAAACT ATCAGCTGGA TTAAGGAGAA AGGGCAGTTG
    ATGGCCTCAG ATGACTTTGG CAGAGACTTG GCCAGCGTGC AAGCTTTACT ACGTAAGCAT
    GAAGGCCTGG AAAGAGATCT TGCAGCTTTG GAGGATAAGG TTAAGGCCCT CTGTGCAGAA
    GCTGACCGTT TGCAGCAGTC TCACCCTATA AATGCTTCTC AAATTCAAGT GAAACGGGAG
    GAACTGATTG CCAACTGGGA GCAGATCCGA ACTCTGGCAG CAGAGAGGCA TGCTCGCCTT
    AATGACTCCT ACAGGTTGCA GCGCTTTCTT GCAGATTTCC GGGACCTCAC TAGCTGGGTA
    ACTGAGATGA AGGCTCTGAT AAATGCTGAT GAGCTTGCCA ATGATGTGGC TGGAGCAGAA
    GCCCTTCTAG ATAGACATCA GGAACATAAG GGAGAAATTG ATGCTCATGA GGATAGCTTC
    AGATCTGCTG ATGAGTCTGG GCAGGCTTTG CTTGCAGCTG GGCACTATGC TTCTGATGAA
    GTTAAAGAAA AGCTGACTAT CCTCTCAGAT GAAAGATCAG CCTTGCTGGA ACTATGGGAG
    CTTCGCAGGC AACAGTATGA GCAGTGCATG GATCTGCAGC TTTTCTACAG AGATACTGAA
    CAAGTTGACA ACTGGATGAG CAAACAAGAA GCTTTTCTGC TGAACGAAGA CCTTGGTGAT
    TCTCTGGATA GCGTGGAGGC TCTTCTAAAG AAGCATGAAG ATTTTGAGAA ATCCCTAAGT
    GCTCAAGAGG AGAAGATCAC AGCACTGGAT GAGTTTGCTA CTAAACTGAT TCAAAATAAC
    CATTATGCCA TGGATGACGT TGCTACGCGC AGAGATGCTC TTCTGAGTCG CCGAAATGCT
    CTTCATGAAA GAGCCATGAA GCGCCGTGCC CAACTGGCAG ACTCTTTCCA TCTCCAGCAG
    TTTTTCAGGG ACTCTGATGA ACTGAAGAGT TGGGTTAATG AAAAAATGAA AACTGCTACC
    GATGAGGCTT ACAAGGATCC ATCCAACTTG CAAGGTAAAG TTCAGAAGCA TCAGGCTTTT
    GAAGCAGAGC TTTCTGCTAA TCAGAGTCGT ATTGATGCAC TGGAGAAAGC TGGCCAGAAG
    TTGATTGATG TCAATCACTA TGCATCTGAT GAGGTGGCAG CTCGCATGAA TGAAGTCATC
    AGCTTGTGGA AGAAACTTCT GGAAGCCACC GAGCTCAAAG GTATAAAGCT GCGTGAAGCC
    AATCAACAGC AGCAATTTAA TCGCAATGTA GAAGACATTG AGTTGTGGCT GTATGAAGTG
    GAGGGTCACT TGGCTTCTGA TGATTATGGA AAAGATCTTA CCAGCGTTCA GAATCTTCAG
    AAGAAACATG CCCTGCTAGA GGCAGATGTT GCTGCCCATC AGGATCGGAT AGATGGCATT
    ACCATCCAGG CACGCCAGTT CCAAGATGCT GGGCACTTTG ATGCTGACAA TATCAAGAAG
    AAACAAGAAG CTTTAGTAGC TCGTTATGAA GCTCTGAAGG ATCCTATGGT GGCTCGCAAG
    CAGAAACTTG CAGATTCTCT TCGCCTGCAG CAGCTTTTCC GTGACATCGA GGATGAAGAG
    ACCTGGATCA GGGAAAAGGA ACCTATTGCA GCCTCAACAA ACCGAGGCAA GGACTTAATT
    GGTGTCCAGA ATCTGCTAAA GAAGCACCAG GCTTTGCAGG CAGAAATTGC AGGCCATGAG
    CCTCGCATTA AAGCAGTCAC ACAAAAAGGA AATGCGATGG TGGAAGAGGG ACATTTTGCT
    GCTGAAGATG TGAAGATCAA ATTGAATGAA CTAAACCAAA AATGGGACTC TCTGAAAGCC
    AAGGCATCTC AACGGCGACA GGATCTAGAG GATTCTCTGC AAGCTCAGCA GTATTTTGCT
    GATGCTAATG AGGCAGAATC ATGGATGAGG GAAAAGGAGC CCATTGTAGG CAGCACGGAT
    TATGGAAAGG ATGAAGACTC TGCTGAGGCT CTCCTGAAGA AGCATGAAGC TTTGATGTCT
    GATCTTTCTG CTTACGGCAG TAGCATACAG GCATTAAGGG AACAGGCCCA GTCCTGCAGG
    CAACAAGTTG CTCCCACTGA TGATGAAACT GGAAAAGAGC TTGTGCTAGC ACTCTATGAT
    TACCAAGAGA AGAGTCCTCG GGAGGTGACT ATGAAGAAAG GAGATATTCT AACCCTGCTC
    AACAGCACCA ACAAGGACTG GTGGAAGGTT GAAGTTAACG ATCGTCAGGG CTTTGTACCA
    GCTGCCTATG TGAAAAAACT AGATCCTGCC CAGTCTGCAT CCCGAGAGAA TCTTCTAGAA
    GAGCAAGGCA GCATAGCATT GCGACAGGAG CAAATTGACA ACCAGACTCT CATTACTAAG
    GAAGTCGGCA GTGTATCTCT GCGTATGAAA CAGGTCGAAG AACTGTATCA TTCCCTTCTT
    GAGCTGGGAG AAAAACGTAA AGGCATGCTA GAAAAAAGCT GCAAGAAGTT TATGCTTTTC
    CGTGAGGCTA ATGAGCTTCA ACAGTGGATC AATGAGAAGG AAGCTGCGCT CACTAATGAG
    GAAGTGGGTG CTGATTTGGA GCAGGTGGAG GTGCTACAGA AGAAGTTTGA TGATTTTCAG
    AAGGATTTAA AAGCTAATGA GTCACGACTG AAGGACATAA ACAAGGTTGC AAATGACCTG
    GAGTCTGAAG GGCTGATGGC AGAGGAAGTG CAAGCAGTAG AGCACCAGGA AGTCTATGGA
    ATGATGCCCA GAGATGAAAC TGATTCTAAG ACAGCCTCTC CTTGGAAGTC TGCACGTATG
    ATGGTACACA CAGTGGCAAC GTTTAACTCA ATCAAGGAAC TGAATGAACG CTGGAGATCC
    CTGCAGCAGT TGGCAGAGGA GCGAAGCCAG CTGTTGGGCA GCGCTCATGA AGTCCAGAGG
    TTCCACAGAG ATGCTGATGA AACAAAAGAA TGGATAGAAG AGAAGAATCA AGCATTAAAT
    ACCGACAACT ATGGACATGA CTTGGCCAGT GTGCAGGCTC TGCAGCGCAA GCATGAAGGC
    TTTGAGAGAG ACTTGGCAGC TCTTGGAGAC AAGGTGAATT CTCTTGGTGA AACTGCCCAG
    CGTCTGATCC AGTCACATCC AGAATCAGCT GAAGATCTTC AAGAAAAATG TACTGAGTTG
    AACCAAGCCT GGAGTAGTCT GGGAAAACGT GCTGACCAAC GCAAAGAGAA GCTTGGAGAT
    TCTCATGACC TGCAGCGTTT CCTCAGTGAT TTTAGAGACC TTATGTCTTG GATCAATGGA
    ATCCGGGGTC TGGTCTCCTC AGATGAACTT GCAAAAGATG TGACTGGAGC TGAAGCTCTG
    TTGGAAAGGC ATCAGGAGCA CCGTACTGAA ATAGATGCAC GGGCTGGCAC TTTTCAGGCA
    TTTGAACAGT TTGGACAGCA GCTTCTGGCT CGTGGACACT ATGCCAGCCC AGAGATTAAG
    GAGAAACTGG ATATTCTAGA TCAAGAACGG ACAGACCTAG AGAAGGCCTG GGTCCAGCGC
    AGAATGATGC TAGACCAGTG CTTAGAACTA CAGCTGTTTC ATCGGGATTG TGAACAAGCT
    GAAAACTGGA TGGCTGCCCG AGAGGCGTTC CTAAATACAG AAGACAAAGG AGACTCCTTA
    GACAGCGTGG AGGCACTCAT CAAGAAGCAT GAAGATTTCG ATAAAGCAAT CAATGTCCAG
    GAAGAGAAAA TTGCTGTCTT GCAGTCTTTT GCTGACCAAC TGATTGCTGC TGATCATTAT
    GCAAAAGGAG TCATTGCTAA CAGACGCAAT GAGGTTCTGG ACAGGTGGCG TCGTCTGAAG
    GCTCAGATGA TTGAGAAGAG ATCTAAGCTG GGAGAATCTC AGACCCTCCA ACAGTTCAGT
    CGTGATGTTG ATGAAATAGA AGCTTGGATC AGTGAAAAGC TTCAAACTGC AAGTGATGAG
    TCATATAAGG ATCCCACAAA CATCCAGCTT TCCAAACTGC TGAGCAAGCA CCAGAAGCAC
    CAAGCCTTTG AAGCTGAGCT CCACGCCAAC GCAGATCGGA TTCGTGGAGT CATTGAAATG
    GGGAACTCTC TTATTGAAAG AGGAGCGTGT GCTGGCAGCG AGGATGCTGT GAAGGCTCGG
    CTGGCTGCCC TGGCTGACCA ATGGGAGTTC CTGGTCCAGA AGTCATCAGA GAAGAGTCAG
    AAACTGAAAG AAGCAAATAA ACAGCAGAAT TTCAATACCG GCATCAAGGA CTTTGATTTC
    TGGCTTTCAG AGGTGGAAGC TTTGTTGGCA TCTGAAGACT ATGGGAAGGA CTTGGCATCA
    GTGAACAACC TTCTGAAGAA GCACCAATTA CTGGAAGCTG ATATATCTGC TCATGAGGAT
    CGGCTGAAGG ACCTGAACAG CCAGGCTGAC AGTTTGATGA CCAGCAGTGC TTTCGATACC
    TCCCAAGTAA AGGATAAACG TGAAACTATA AATGGGCGCT TCCAGAGAAT CAAGAGCATG
    GCAGCTGCCC GCCGTGCGAA GCTCAACGAG TCGCACCGCT TGCATCAGTT CTTCCGTGAC
    ATGGATGATG AGGAGTCCTG GATCAAAGAG AAGAAACTGT TGGTGAGCTC AGAGGACTAT
    GGCAGAGACC TGACTGGTGT GCAGAACCTG AGGAAGAAAC ATAAGCGCTT GGAAGCAGAA
    TTAGCTGCCC ATGAACCTGC TATCCAGGGT GTTCTAGACA CTGGCAAGAA GCTTTCAGAT
    GATAACACAA TTGGGAAGGA GGAGATACAG CAAAGACTGG CTCAGTTTGT GGACCACTGG
    AAAGAGTTAA AACAGCTGGC AGCTGCTCGG GGGCAGCGTC TGGAGGAGTC CCTGGAGTAC
    CAACAGTTTG TAGCAAATGT TGAGGAAGAG GAGGCATGGA TCAACGAGAA AATGACATTG
    GTAGCCAGTG AGGATTATGG AGACACACTT GCTGCTATCC AGGGCTTGCT GAAGAAGCAT
    GAAGCATTCG AGACTGACTT TACTGTCCAC AAAGACAGAG TGAATGATGT TTGCGCTAAT
    GGAGAGGATC TCATTAAAAA GAATAATCAC CATGTGGAGA ACATTACTGC TAAGATGAAG
    GGCCTCAAGG GCAAGGTATC AGATCTGGAG AAAGCCGCAG CTCAGAGGAA AGCCAAACTG
    GATGAGAACT CTGCCTTCCT CCAGTTCAAC TGGAAAGCAG ATGTGGTGGA GTCATGGATA
    GGTGAGAAGG AAAACAGTCT GAAGACAGAT GATTATGGCC GTGACCTCTC CTCTGTGCAA
    ACACTACTCA CCAAACAGGA AACCTTTGAT GCTGGACTTC AGGCTTTCCA GCAGGAGGGA
    ATTGCAAACA TCACTGCTCT GAAAGACCAG CTACTGGCAG CCAAACATAT CCAATCAAAG
    GCCATTGAGG TTCGTCACGC TTCCTTGATG AAACGCTGGA ATCAGCTGCT TGCTAATTCT
    GCAGCCAGGA AAAAGAAACT CTTGGAGGCT CAGGAGCACT TCAGAAAGGT TGAAGATCTC
    TTCTTGACTT TTGCCAAGAA GGCCTCTGCC TTCAACAGTT GGTTTGAGAA TGCTGAGGAG
    GACCTGACGG ATCCCGTGCG CTGCAATTCC CTGGAAGAAA TCAAAGCACT GCGAGAAGCC
    CACGATGCGT TCCGTTCCTC ACTTAGCTCT GCCCAAGCTG ACTTCAACCA GCTGGCAGAG
    CTTGATCGGC AGATCAAGAG CTTCCGTGTA GCCTCCAACC CGTACACTTG GTTTACTATG
    GAGGCTCTTG AAGAAACTTG GAGGAATCTG CAGAAAATTA TCAAGGAGCG TGAATTGGAG
    TTGCAGAAGG AGCAGCGGAG GCAGGAAGAA AATGACAAGT TGCGCCAGGA GTTTGCTCAG
    CATGCTAATG CCTTCCACCA GTGGATTCAG GAGACAAGGA CTTACCTGCT AGATGGCATA
    GCCTATCGTC GTGTCATTCG TGTCTATCAG TATGAAGTTG GGGATGATCT ATCTGGAAGG
    TCCTGCATGG TGGAGGAGTC GGGAACACTG GAATCCCAGC TGGAAGCTAC TAAACGCAAG
    CACCAGGAGA TCCGGGCTAT GAGGAGCCAG CTGAAGAAGA TTGAGGACCT TGGAGCAGCC
    ATGGAAGAGG CACTTATCTT GGACAACAAA TACACAGAGC ACAGCACCGT GGGGCTGGCG
    CAGCAGTGGG ACCAGCTTGA CCAGCTGGGA ATGAGAATGC AGCACAATCT GGAACAGCAG
    ATTCAAGCTC GAAACACAAC TGGAGTCACA GAGGAGGCCC TGAAGGAGTT CAGCATGATG
    TTCAAGCACT TTGACAAGGA CAAATCTGGA CGTCTTAATC ACCAGGAGTT TAAGTCTTGC
    TTGCGCTCCC TTGGCTATGA CCTGCCCATG GTTGAGGAAG GAGAGCCTGA CCCCGAATTT
    GAGTCTATTC TTGACACTGT TGATCCCAAC AGGGATGGCC ACGTCTCGCT GCAGGAGTAC
    ATGGCGTTCA TGATCAGCAG GGAAACAGAG AACGTGAAAT CCAGCGAGGA GATTGAGAGT
    GCTTTCCGTG CCCTCAGCTC GGAGGGGAAG CCTTACGTGA CCAAGGAGGA GCTCTACCAG
    AACCTGACCC GGGAGCAGGC CGACTACTGC ATTTCTCACA TGAAGCCCTA CATGGATGGC
    AAGGGCAGAG AACTTCCTTC TGCCTACGAC TACATAGAAT TTACACGTTC ACTCTTTGTG
    AATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa43311
    Clone ID Related Accession (Same CDS sequence) XM_015279285.2 , XM_015279285.1
    Accession Version XM_015279285.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7542bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015279285.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
    TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG
    TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC
    TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT
    GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT
    GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG
    ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAT CTTTCACGCC TTACAGGACT
    GCACAGCGCC CAATAGTAAC AGCCACCCAA GTTGGGTCCC ACATACGAAC TCGTCTACAG
    GAGCTGCACC GACTATGGGA GTTACTGCTG GAAAAAATGA GAGAGAAGGG AGTGAAGCTG
    TTGCAAGCAC AGAAGCTGGT GCAATTCTTA CGGGAATGTG AAGATGTCAT GGACTGGATC
    AATGACAAGG AAGCAATAGT GACCTCAGAA GAGCTTGGAC AGGACTTAGA GCATGTTGAG
    GTTTTGCAGA AGAAATTTGA AGAGTTCCAA ACAGATCTGG CAGCTCATGA GGAGAGAGTG
    AATGAAGTGA ACCAGTTCGC TGGCAAACTT ATCCAGGAAC AGCACCCTGA GGAGGAACTT
    ATAAAGTCCA AACAGGATGA AGTAAATGCA AGCTGGCAGC GTCTTAAGGG GCTTGCCCAA
    CAGAGGCAAG GAAAACTCTT TGGGGCAGCT GAAGTTCAGC GCTTCAACAG GGATGTGGAT
    GAAACTATCA GCTGGATTAA GGAGAAAGGG CAGTTGATGG CCTCAGATGA CTTTGGCAGA
    GACTTGGCCA GCGTGCAAGC TTTACTACGT AAGCATGAAG GCCTGGAAAG AGATCTTGCA
    GCTTTGGAGG ATAAGGTTAA GGCCCTCTGT GCAGAAGCTG ACCGTTTGCA GCAGTCTCAC
    CCTATAAATG CTTCTCAAAT TCAAGTGAAA CGGGAGGAAC TGATTGCCAA CTGGGAGCAG
    ATCCGAACTC TGGCAGCAGA GAGGCATGCT CGCCTTAATG ACTCCTACAG GTTGCAGCGC
    TTTCTTGCAG ATTTCCGGGA CCTCACTAGC TGGGTAACTG AGATGAAGGC TCTGATAAAT
    GCTGATGAGC TTGCCAATGA TGTGGCTGGA GCAGAAGCCC TTCTAGATAG ACATCAGGAA
    CATAAGGGAG AAATTGATGC TCATGAGGAT AGCTTCAGAT CTGCTGATGA GTCTGGGCAG
    GCTTTGCTTG CAGCTGGGCA CTATGCTTCT GATGAAGTTA AAGAAAAGCT GACTATCCTC
    TCAGATGAAA GATCAGCCTT GCTGGAACTA TGGGAGCTTC GCAGGCAACA GTATGAGCAG
    TGCATGGATC TGCAGCTTTT CTACAGAGAT ACTGAACAAG TTGACAACTG GATGAGCAAA
    CAAGAAGCTT TTCTGCTGAA CGAAGACCTT GGTGATTCTC TGGATAGCGT GGAGGCTCTT
    CTAAAGAAGC ATGAAGATTT TGAGAAATCC CTAAGTGCTC AAGAGGAGAA GATCACAGCA
    CTGGATGAGT TTGCTACTAA ACTGATTCAA AATAACCATT ATGCCATGGA TGACGTTGCT
    ACGCGCAGAG ATGCTCTTCT GAGTCGCCGA AATGCTCTTC ATGAAAGAGC CATGAAGCGC
    CGTGCCCAAC TGGCAGACTC TTTCCATCTC CAGCAGTTTT TCAGGGACTC TGATGAACTG
    AAGAGTTGGG TTAATGAAAA AATGAAAACT GCTACCGATG AGGCTTACAA GGATCCATCC
    AACTTGCAAG GTAAAGTTCA GAAGCATCAG GCTTTTGAAG CAGAGCTTTC TGCTAATCAG
    AGTCGTATTG ATGCACTGGA GAAAGCTGGC CAGAAGTTGA TTGATGTCAA TCACTATGCA
    TCTGATGAGG TGGCAGCTCG CATGAATGAA GTCATCAGCT TGTGGAAGAA ACTTCTGGAA
    GCCACCGAGC TCAAAGGTAT AAAGCTGCGT GAAGCCAATC AACAGCAGCA ATTTAATCGC
    AATGTAGAAG ACATTGAGTT GTGGCTGTAT GAAGTGGAGG GTCACTTGGC TTCTGATGAT
    TATGGAAAAG ATCTTACCAG CGTTCAGAAT CTTCAGAAGA AACATGCCCT GCTAGAGGCA
    GATGTTGCTG CCCATCAGGA TCGGATAGAT GGCATTACCA TCCAGGCACG CCAGTTCCAA
    GATGCTGGGC ACTTTGATGC TGACAATATC AAGAAGAAAC AAGAAGCTTT AGTAGCTCGT
    TATGAAGCTC TGAAGGATCC TATGGTGGCT CGCAAGCAGA AACTTGCAGA TTCTCTTCGC
    CTGCAGCAGC TTTTCCGTGA CATCGAGGAT GAAGAGACCT GGATCAGGGA AAAGGAACCT
    ATTGCAGCCT CAACAAACCG AGGCAAGGAC TTAATTGGTG TCCAGAATCT GCTAAAGAAG
    CACCAGGCTT TGCAGGCAGA AATTGCAGGC CATGAGCCTC GCATTAAAGC AGTCACACAA
    AAAGGAAATG CGATGGTGGA AGAGGGACAT TTTGCTGCTG AAGATGTGAA GATCAAATTG
    AATGAACTAA ACCAAAAATG GGACTCTCTG AAAGCCAAGG CATCTCAACG GCGACAGGAT
    CTAGAGGATT CTCTGCAAGC TCAGCAGTAT TTTGCTGATG CTAATGAGGC AGAATCATGG
    ATGAGGGAAA AGGAGCCCAT TGTAGGCAGC ACGGATTATG GAAAGGATGA AGACTCTGCT
    GAGGCTCTCC TGAAGAAGCA TGAAGCTTTG ATGTCTGATC TTTCTGCTTA CGGCAGTAGC
    ATACAGGCAT TAAGGGAACA GGCCCAGTCC TGCAGGCAAC AAGTTGCTCC CACTGATGAT
    GAAACTGGAA AAGAGCTTGT GCTAGCACTC TATGATTACC AAGAGAAGAG TCCTCGGGAG
    GTGACTATGA AGAAAGGAGA TATTCTAACC CTGCTCAACA GCACCAACAA GGACTGGTGG
    AAGGTTGAAG TTAACGATCG TCAGGGCTTT GTACCAGCTG CCTATGTGAA AAAACTAGAT
    CCTGCCCAGT CTGCATCCCG AGAGAATCTT CTAGAAGAGC AAGGCAGCAT AGCATTGCGA
    CAGGAGCAAA TTGACAACCA GTATCATTCC CTTCTTGAGC TGGGAGAAAA ACGTAAAGGC
    ATGCTAGAAA AAAGCTGCAA GAAGTTTATG CTTTTCCGTG AGGCTAATGA GCTTCAACAG
    TGGATCAATG AGAAGGAAGC TGCGCTCACT AATGAGGAAG TGGGTGCTGA TTTGGAGCAG
    GTGGAGGTGC TACAGAAGAA GTTTGATGAT TTTCAGAAGG ATTTAAAAGC TAATGAGTCA
    CGACTGAAGG ACATAAACAA GGTTGCAAAT GACCTGGAGT CTGAAGGGCT GATGGCAGAG
    GAAGTGCAAG CAGTAGAGCA CCAGGAAGTC TATGGAATGA TGCCCAGAGA TGAAACTGAT
    TCTAAGACAG CCTCTCCTTG GAAGTCTGCA CGTATGATGG TACACACAGT GGCAACGTTT
    AACTCAATCA AGGAACTGAA TGAACGCTGG AGATCCCTGC AGCAGTTGGC AGAGGAGCGA
    AGCCAGCTGT TGGGCAGCGC TCATGAAGTC CAGAGGTTCC ACAGAGATGC TGATGAAACA
    AAAGAATGGA TAGAAGAGAA GAATCAAGCA TTAAATACCG ACAACTATGG ACATGACTTG
    GCCAGTGTGC AGGCTCTGCA GCGCAAGCAT GAAGGCTTTG AGAGAGACTT GGCAGCTCTT
    GGAGACAAGG TGAATTCTCT TGGTGAAACT GCCCAGCGTC TGATCCAGTC ACATCCAGAA
    TCAGCTGAAG ATCTTCAAGA AAAATGTACT GAGTTGAACC AAGCCTGGAG TAGTCTGGGA
    AAACGTGCTG ACCAACGCAA AGAGAAGCTT GGAGATTCTC ATGACCTGCA GCGTTTCCTC
    AGTGATTTTA GAGACCTTAT GTCTTGGATC AATGGAATCC GGGGTCTGGT CTCCTCAGAT
    GAACTTGCAA AAGATGTGAC TGGAGCTGAA GCTCTGTTGG AAAGGCATCA GGAGCACCGT
    ACTGAAATAG ATGCACGGGC TGGCACTTTT CAGGCATTTG AACAGTTTGG ACAGCAGCTT
    CTGGCTCGTG GACACTATGC CAGCCCAGAG ATTAAGGAGA AACTGGATAT TCTAGATCAA
    GAACGGACAG ACCTAGAGAA GGCCTGGGTC CAGCGCAGAA TGATGCTAGA CCAGTGCTTA
    GAACTACAGC TGTTTCATCG GGATTGTGAA CAAGCTGAAA ACTGGATGGC TGCCCGAGAG
    GCGTTCCTAA ATACAGAAGA CAAAGGAGAC TCCTTAGACA GCGTGGAGGC ACTCATCAAG
    AAGCATGAAG ATTTCGATAA AGCAATCAAT GTCCAGGAAG AGAAAATTGC TGTCTTGCAG
    TCTTTTGCTG ACCAACTGAT TGCTGCTGAT CATTATGCAA AAGGAGTCAT TGCTAACAGA
    CGCAATGAGG TTCTGGACAG GTGGCGTCGT CTGAAGGCTC AGATGATTGA GAAGAGATCT
    AAGCTGGGAG AATCTCAGAC CCTCCAACAG TTCAGTCGTG ATGTTGATGA AATAGAAGCT
    TGGATCAGTG AAAAGCTTCA AACTGCAAGT GATGAGTCAT ATAAGGATCC CACAAACATC
    CAGCTTTCCA AACTGCTGAG CAAGCACCAG AAGCACCAAG CCTTTGAAGC TGAGCTCCAC
    GCCAACGCAG ATCGGATTCG TGGAGTCATT GAAATGGGGA ACTCTCTTAT TGAAAGAGGA
    GCGTGTGCTG GCAGCGAGGA TGCTGTGAAG GCTCGGCTGG CTGCCCTGGC TGACCAATGG
    GAGTTCCTGG TCCAGAAGTC ATCAGAGAAG AGTCAGAAAC TGAAAGAAGC AAATAAACAG
    CAGAATTTCA ATACCGGCAT CAAGGACTTT GATTTCTGGC TTTCAGAGGT GGAAGCTTTG
    TTGGCATCTG AAGACTATGG GAAGGACTTG GCATCAGTGA ACAACCTTCT GAAGAAGCAC
    CAATTACTGG AAGCTGATAT ATCTGCTCAT GAGGATCGGC TGAAGGACCT GAACAGCCAG
    GCTGACAGTT TGATGACCAG CAGTGCTTTC GATACCTCCC AAGTAAAGGA TAAACGTGAA
    ACTATAAATG GGCGCTTCCA GAGAATCAAG AGCATGGCAG CTGCCCGCCG TGCGAAGCTC
    AACGAGTCGC ACCGCTTGCA TCAGTTCTTC CGTGACATGG ATGATGAGGA GTCCTGGATC
    AAAGAGAAGA AACTGTTGGT GAGCTCAGAG GACTATGGCA GAGACCTGAC TGGTGTGCAG
    AACCTGAGGA AGAAACATAA GCGCTTGGAA GCAGAATTAG CTGCCCATGA ACCTGCTATC
    CAGGGTGTTC TAGACACTGG CAAGAAGCTT TCAGATGATA ACACAATTGG GAAGGAGGAG
    ATACAGCAAA GACTGGCTCA GTTTGTGGAC CACTGGAAAG AGTTAAAACA GCTGGCAGCT
    GCTCGGGGGC AGCGTCTGGA GGAGTCCCTG GAGTACCAAC AGTTTGTAGC AAATGTTGAG
    GAAGAGGAGG CATGGATCAA CGAGAAAATG ACATTGGTAG CCAGTGAGGA TTATGGAGAC
    ACACTTGCTG CTATCCAGGG CTTGCTGAAG AAGCATGAAG CATTCGAGAC TGACTTTACT
    GTCCACAAAG ACAGAGTGAA TGATGTTTGC GCTAATGGAG AGGATCTCAT TAAAAAGAAT
    AATCACCATG TGGAGAACAT TACTGCTAAG ATGAAGGGCC TCAAGGGCAA GGTATCAGAT
    CTGGAGAAAG CCGCAGCTCA GAGGAAAGCC AAACTGGATG AGAACTCTGC CTTCCTCCAG
    TTCAACTGGA AAGCAGATGT GGTGGAGTCA TGGATAGGTG AGAAGGAAAA CAGTCTGAAG
    ACAGATGATT ATGGCCGTGA CCTCTCCTCT GTGCAAACAC TACTCACCAA ACAGGAAACC
    TTTGATGCTG GACTTCAGGC TTTCCAGCAG GAGGGAATTG CAAACATCAC TGCTCTGAAA
    GACCAGCTAC TGGCAGCCAA ACATATCCAA TCAAAGGCCA TTGAGGTTCG TCACGCTTCC
    TTGATGAAAC GCTGGAATCA GCTGCTTGCT AATTCTGCAG CCAGGAAAAA GAAACTCTTG
    GAGGCTCAGG AGCACTTCAG AAAGGTTGAA GATCTCTTCT TGACTTTTGC CAAGAAGGCC
    TCTGCCTTCA ACAGTTGGTT TGAGAATGCT GAGGAGGACC TGACGGATCC CGTGCGCTGC
    AATTCCCTGG AAGAAATCAA AGCACTGCGA GAAGCCCACG ATGCGTTCCG TTCCTCACTT
    AGCTCTGCCC AAGCTGACTT CAACCAGCTG GCAGAGCTTG ATCGGCAGAT CAAGAGCTTC
    CGTGTAGCCT CCAACCCGTA CACTTGGTTT ACTATGGAGG CTCTTGAAGA AACTTGGAGG
    AATCTGCAGA AAATTATCAA GGAGCGTGAA TTGGAGTTGC AGAAGGAGCA GCGGAGGCAG
    GAAGAAAATG ACAAGTTGCG CCAGGAGTTT GCTCAGCATG CTAATGCCTT CCACCAGTGG
    ATTCAGGAGA CAAGGACTTA CCTGCTAGAT GGCATAGCCT ATCGTCGTGT CATTCGTGTC
    TATCAGTATG AAGTTGGGGA TGATCTATCT GGAAGGTCCT GCATGGTGGA GGAGTCGGGA
    ACACTGGAAT CCCAGCTGGA AGCTACTAAA CGCAAGCACC AGGAGATCCG GGCTATGAGG
    AGCCAGCTGA AGAAGATTGA GGACCTTGGA GCAGCCATGG AAGAGGCACT TATCTTGGAC
    AACAAATACA CAGAGCACAG CACCGTGGGG CTGGCGCAGC AGTGGGACCA GCTTGACCAG
    CTGGGAATGA GAATGCAGCA CAATCTGGAA CAGCAGATTC AAGCTCGAAA CACAACTGGA
    GTCACAGAGG AGGCCCTGAA GGAGTTCAGC ATGATGTTCA AGCACTTTGA CAAGGACAAA
    TCTGGACGTC TTAATCACCA GGAGTTTAAG TCTTGCTTGC GCTCCCTTGG CTATGACCTG
    CCCATGGTTG AGGAAGGAGA GCCTGACCCC GAATTTGAGT CTATTCTTGA CACTGTTGAT
    CCCAACAGGG ATGGCCACGT CTCGCTGCAG GAGTACATGG CGTTCATGAT CAGCAGGGAA
    ACAGAGAACG TGAAATCCAG CGAGGAGATT GAGAGTGCTT TCCGTGCCCT CAGCTCGGAG
    GGGAAGCCTT ACGTGACCAA GGAGGAGCTC TACCAGAACC TGACCCGGGA GCAGGCCGAC
    TACTGCATTT CTCACATGAA GCCCTACATG GATGGCAAGG GCAGAGAACT TCCTTCTGCC
    TACGACTACA TAGAATTTAC ACGTTCACTC TTTGTGAATT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015134771.1
    CDS18..7559
    Translation

    Target ORF information:

    RefSeq Version XM_015279285.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015279285.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
    TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG
    TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC
    TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT
    GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT
    GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG
    ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAT CTTTCACGCC TTACAGGACT
    GCACAGCGCC CAATAGTAAC AGCCACCCAA GTTGGGTCCC ACATACGAAC TCGTCTACAG
    GAGCTGCACC GACTATGGGA GTTACTGCTG GAAAAAATGA GAGAGAAGGG AGTGAAGCTG
    TTGCAAGCAC AGAAGCTGGT GCAATTCTTA CGGGAATGTG AAGATGTCAT GGACTGGATC
    AATGACAAGG AAGCAATAGT GACCTCAGAA GAGCTTGGAC AGGACTTAGA GCATGTTGAG
    GTTTTGCAGA AGAAATTTGA AGAGTTCCAA ACAGATCTGG CAGCTCATGA GGAGAGAGTG
    AATGAAGTGA ACCAGTTCGC TGGCAAACTT ATCCAGGAAC AGCACCCTGA GGAGGAACTT
    ATAAAGTCCA AACAGGATGA AGTAAATGCA AGCTGGCAGC GTCTTAAGGG GCTTGCCCAA
    CAGAGGCAAG GAAAACTCTT TGGGGCAGCT GAAGTTCAGC GCTTCAACAG GGATGTGGAT
    GAAACTATCA GCTGGATTAA GGAGAAAGGG CAGTTGATGG CCTCAGATGA CTTTGGCAGA
    GACTTGGCCA GCGTGCAAGC TTTACTACGT AAGCATGAAG GCCTGGAAAG AGATCTTGCA
    GCTTTGGAGG ATAAGGTTAA GGCCCTCTGT GCAGAAGCTG ACCGTTTGCA GCAGTCTCAC
    CCTATAAATG CTTCTCAAAT TCAAGTGAAA CGGGAGGAAC TGATTGCCAA CTGGGAGCAG
    ATCCGAACTC TGGCAGCAGA GAGGCATGCT CGCCTTAATG ACTCCTACAG GTTGCAGCGC
    TTTCTTGCAG ATTTCCGGGA CCTCACTAGC TGGGTAACTG AGATGAAGGC TCTGATAAAT
    GCTGATGAGC TTGCCAATGA TGTGGCTGGA GCAGAAGCCC TTCTAGATAG ACATCAGGAA
    CATAAGGGAG AAATTGATGC TCATGAGGAT AGCTTCAGAT CTGCTGATGA GTCTGGGCAG
    GCTTTGCTTG CAGCTGGGCA CTATGCTTCT GATGAAGTTA AAGAAAAGCT GACTATCCTC
    TCAGATGAAA GATCAGCCTT GCTGGAACTA TGGGAGCTTC GCAGGCAACA GTATGAGCAG
    TGCATGGATC TGCAGCTTTT CTACAGAGAT ACTGAACAAG TTGACAACTG GATGAGCAAA
    CAAGAAGCTT TTCTGCTGAA CGAAGACCTT GGTGATTCTC TGGATAGCGT GGAGGCTCTT
    CTAAAGAAGC ATGAAGATTT TGAGAAATCC CTAAGTGCTC AAGAGGAGAA GATCACAGCA
    CTGGATGAGT TTGCTACTAA ACTGATTCAA AATAACCATT ATGCCATGGA TGACGTTGCT
    ACGCGCAGAG ATGCTCTTCT GAGTCGCCGA AATGCTCTTC ATGAAAGAGC CATGAAGCGC
    CGTGCCCAAC TGGCAGACTC TTTCCATCTC CAGCAGTTTT TCAGGGACTC TGATGAACTG
    AAGAGTTGGG TTAATGAAAA AATGAAAACT GCTACCGATG AGGCTTACAA GGATCCATCC
    AACTTGCAAG GTAAAGTTCA GAAGCATCAG GCTTTTGAAG CAGAGCTTTC TGCTAATCAG
    AGTCGTATTG ATGCACTGGA GAAAGCTGGC CAGAAGTTGA TTGATGTCAA TCACTATGCA
    TCTGATGAGG TGGCAGCTCG CATGAATGAA GTCATCAGCT TGTGGAAGAA ACTTCTGGAA
    GCCACCGAGC TCAAAGGTAT AAAGCTGCGT GAAGCCAATC AACAGCAGCA ATTTAATCGC
    AATGTAGAAG ACATTGAGTT GTGGCTGTAT GAAGTGGAGG GTCACTTGGC TTCTGATGAT
    TATGGAAAAG ATCTTACCAG CGTTCAGAAT CTTCAGAAGA AACATGCCCT GCTAGAGGCA
    GATGTTGCTG CCCATCAGGA TCGGATAGAT GGCATTACCA TCCAGGCACG CCAGTTCCAA
    GATGCTGGGC ACTTTGATGC TGACAATATC AAGAAGAAAC AAGAAGCTTT AGTAGCTCGT
    TATGAAGCTC TGAAGGATCC TATGGTGGCT CGCAAGCAGA AACTTGCAGA TTCTCTTCGC
    CTGCAGCAGC TTTTCCGTGA CATCGAGGAT GAAGAGACCT GGATCAGGGA AAAGGAACCT
    ATTGCAGCCT CAACAAACCG AGGCAAGGAC TTAATTGGTG TCCAGAATCT GCTAAAGAAG
    CACCAGGCTT TGCAGGCAGA AATTGCAGGC CATGAGCCTC GCATTAAAGC AGTCACACAA
    AAAGGAAATG CGATGGTGGA AGAGGGACAT TTTGCTGCTG AAGATGTGAA GATCAAATTG
    AATGAACTAA ACCAAAAATG GGACTCTCTG AAAGCCAAGG CATCTCAACG GCGACAGGAT
    CTAGAGGATT CTCTGCAAGC TCAGCAGTAT TTTGCTGATG CTAATGAGGC AGAATCATGG
    ATGAGGGAAA AGGAGCCCAT TGTAGGCAGC ACGGATTATG GAAAGGATGA AGACTCTGCT
    GAGGCTCTCC TGAAGAAGCA TGAAGCTTTG ATGTCTGATC TTTCTGCTTA CGGCAGTAGC
    ATACAGGCAT TAAGGGAACA GGCCCAGTCC TGCAGGCAAC AAGTTGCTCC CACTGATGAT
    GAAACTGGAA AAGAGCTTGT GCTAGCACTC TATGATTACC AAGAGAAGAG TCCTCGGGAG
    GTGACTATGA AGAAAGGAGA TATTCTAACC CTGCTCAACA GCACCAACAA GGACTGGTGG
    AAGGTTGAAG TTAACGATCG TCAGGGCTTT GTACCAGCTG CCTATGTGAA AAAACTAGAT
    CCTGCCCAGT CTGCATCCCG AGAGAATCTT CTAGAAGAGC AAGGCAGCAT AGCATTGCGA
    CAGGAGCAAA TTGACAACCA GTATCATTCC CTTCTTGAGC TGGGAGAAAA ACGTAAAGGC
    ATGCTAGAAA AAAGCTGCAA GAAGTTTATG CTTTTCCGTG AGGCTAATGA GCTTCAACAG
    TGGATCAATG AGAAGGAAGC TGCGCTCACT AATGAGGAAG TGGGTGCTGA TTTGGAGCAG
    GTGGAGGTGC TACAGAAGAA GTTTGATGAT TTTCAGAAGG ATTTAAAAGC TAATGAGTCA
    CGACTGAAGG ACATAAACAA GGTTGCAAAT GACCTGGAGT CTGAAGGGCT GATGGCAGAG
    GAAGTGCAAG CAGTAGAGCA CCAGGAAGTC TATGGAATGA TGCCCAGAGA TGAAACTGAT
    TCTAAGACAG CCTCTCCTTG GAAGTCTGCA CGTATGATGG TACACACAGT GGCAACGTTT
    AACTCAATCA AGGAACTGAA TGAACGCTGG AGATCCCTGC AGCAGTTGGC AGAGGAGCGA
    AGCCAGCTGT TGGGCAGCGC TCATGAAGTC CAGAGGTTCC ACAGAGATGC TGATGAAACA
    AAAGAATGGA TAGAAGAGAA GAATCAAGCA TTAAATACCG ACAACTATGG ACATGACTTG
    GCCAGTGTGC AGGCTCTGCA GCGCAAGCAT GAAGGCTTTG AGAGAGACTT GGCAGCTCTT
    GGAGACAAGG TGAATTCTCT TGGTGAAACT GCCCAGCGTC TGATCCAGTC ACATCCAGAA
    TCAGCTGAAG ATCTTCAAGA AAAATGTACT GAGTTGAACC AAGCCTGGAG TAGTCTGGGA
    AAACGTGCTG ACCAACGCAA AGAGAAGCTT GGAGATTCTC ATGACCTGCA GCGTTTCCTC
    AGTGATTTTA GAGACCTTAT GTCTTGGATC AATGGAATCC GGGGTCTGGT CTCCTCAGAT
    GAACTTGCAA AAGATGTGAC TGGAGCTGAA GCTCTGTTGG AAAGGCATCA GGAGCACCGT
    ACTGAAATAG ATGCACGGGC TGGCACTTTT CAGGCATTTG AACAGTTTGG ACAGCAGCTT
    CTGGCTCGTG GACACTATGC CAGCCCAGAG ATTAAGGAGA AACTGGATAT TCTAGATCAA
    GAACGGACAG ACCTAGAGAA GGCCTGGGTC CAGCGCAGAA TGATGCTAGA CCAGTGCTTA
    GAACTACAGC TGTTTCATCG GGATTGTGAA CAAGCTGAAA ACTGGATGGC TGCCCGAGAG
    GCGTTCCTAA ATACAGAAGA CAAAGGAGAC TCCTTAGACA GCGTGGAGGC ACTCATCAAG
    AAGCATGAAG ATTTCGATAA AGCAATCAAT GTCCAGGAAG AGAAAATTGC TGTCTTGCAG
    TCTTTTGCTG ACCAACTGAT TGCTGCTGAT CATTATGCAA AAGGAGTCAT TGCTAACAGA
    CGCAATGAGG TTCTGGACAG GTGGCGTCGT CTGAAGGCTC AGATGATTGA GAAGAGATCT
    AAGCTGGGAG AATCTCAGAC CCTCCAACAG TTCAGTCGTG ATGTTGATGA AATAGAAGCT
    TGGATCAGTG AAAAGCTTCA AACTGCAAGT GATGAGTCAT ATAAGGATCC CACAAACATC
    CAGCTTTCCA AACTGCTGAG CAAGCACCAG AAGCACCAAG CCTTTGAAGC TGAGCTCCAC
    GCCAACGCAG ATCGGATTCG TGGAGTCATT GAAATGGGGA ACTCTCTTAT TGAAAGAGGA
    GCGTGTGCTG GCAGCGAGGA TGCTGTGAAG GCTCGGCTGG CTGCCCTGGC TGACCAATGG
    GAGTTCCTGG TCCAGAAGTC ATCAGAGAAG AGTCAGAAAC TGAAAGAAGC AAATAAACAG
    CAGAATTTCA ATACCGGCAT CAAGGACTTT GATTTCTGGC TTTCAGAGGT GGAAGCTTTG
    TTGGCATCTG AAGACTATGG GAAGGACTTG GCATCAGTGA ACAACCTTCT GAAGAAGCAC
    CAATTACTGG AAGCTGATAT ATCTGCTCAT GAGGATCGGC TGAAGGACCT GAACAGCCAG
    GCTGACAGTT TGATGACCAG CAGTGCTTTC GATACCTCCC AAGTAAAGGA TAAACGTGAA
    ACTATAAATG GGCGCTTCCA GAGAATCAAG AGCATGGCAG CTGCCCGCCG TGCGAAGCTC
    AACGAGTCGC ACCGCTTGCA TCAGTTCTTC CGTGACATGG ATGATGAGGA GTCCTGGATC
    AAAGAGAAGA AACTGTTGGT GAGCTCAGAG GACTATGGCA GAGACCTGAC TGGTGTGCAG
    AACCTGAGGA AGAAACATAA GCGCTTGGAA GCAGAATTAG CTGCCCATGA ACCTGCTATC
    CAGGGTGTTC TAGACACTGG CAAGAAGCTT TCAGATGATA ACACAATTGG GAAGGAGGAG
    ATACAGCAAA GACTGGCTCA GTTTGTGGAC CACTGGAAAG AGTTAAAACA GCTGGCAGCT
    GCTCGGGGGC AGCGTCTGGA GGAGTCCCTG GAGTACCAAC AGTTTGTAGC AAATGTTGAG
    GAAGAGGAGG CATGGATCAA CGAGAAAATG ACATTGGTAG CCAGTGAGGA TTATGGAGAC
    ACACTTGCTG CTATCCAGGG CTTGCTGAAG AAGCATGAAG CATTCGAGAC TGACTTTACT
    GTCCACAAAG ACAGAGTGAA TGATGTTTGC GCTAATGGAG AGGATCTCAT TAAAAAGAAT
    AATCACCATG TGGAGAACAT TACTGCTAAG ATGAAGGGCC TCAAGGGCAA GGTATCAGAT
    CTGGAGAAAG CCGCAGCTCA GAGGAAAGCC AAACTGGATG AGAACTCTGC CTTCCTCCAG
    TTCAACTGGA AAGCAGATGT GGTGGAGTCA TGGATAGGTG AGAAGGAAAA CAGTCTGAAG
    ACAGATGATT ATGGCCGTGA CCTCTCCTCT GTGCAAACAC TACTCACCAA ACAGGAAACC
    TTTGATGCTG GACTTCAGGC TTTCCAGCAG GAGGGAATTG CAAACATCAC TGCTCTGAAA
    GACCAGCTAC TGGCAGCCAA ACATATCCAA TCAAAGGCCA TTGAGGTTCG TCACGCTTCC
    TTGATGAAAC GCTGGAATCA GCTGCTTGCT AATTCTGCAG CCAGGAAAAA GAAACTCTTG
    GAGGCTCAGG AGCACTTCAG AAAGGTTGAA GATCTCTTCT TGACTTTTGC CAAGAAGGCC
    TCTGCCTTCA ACAGTTGGTT TGAGAATGCT GAGGAGGACC TGACGGATCC CGTGCGCTGC
    AATTCCCTGG AAGAAATCAA AGCACTGCGA GAAGCCCACG ATGCGTTCCG TTCCTCACTT
    AGCTCTGCCC AAGCTGACTT CAACCAGCTG GCAGAGCTTG ATCGGCAGAT CAAGAGCTTC
    CGTGTAGCCT CCAACCCGTA CACTTGGTTT ACTATGGAGG CTCTTGAAGA AACTTGGAGG
    AATCTGCAGA AAATTATCAA GGAGCGTGAA TTGGAGTTGC AGAAGGAGCA GCGGAGGCAG
    GAAGAAAATG ACAAGTTGCG CCAGGAGTTT GCTCAGCATG CTAATGCCTT CCACCAGTGG
    ATTCAGGAGA CAAGGACTTA CCTGCTAGAT GGCATAGCCT ATCGTCGTGT CATTCGTGTC
    TATCAGTATG AAGTTGGGGA TGATCTATCT GGAAGGTCCT GCATGGTGGA GGAGTCGGGA
    ACACTGGAAT CCCAGCTGGA AGCTACTAAA CGCAAGCACC AGGAGATCCG GGCTATGAGG
    AGCCAGCTGA AGAAGATTGA GGACCTTGGA GCAGCCATGG AAGAGGCACT TATCTTGGAC
    AACAAATACA CAGAGCACAG CACCGTGGGG CTGGCGCAGC AGTGGGACCA GCTTGACCAG
    CTGGGAATGA GAATGCAGCA CAATCTGGAA CAGCAGATTC AAGCTCGAAA CACAACTGGA
    GTCACAGAGG AGGCCCTGAA GGAGTTCAGC ATGATGTTCA AGCACTTTGA CAAGGACAAA
    TCTGGACGTC TTAATCACCA GGAGTTTAAG TCTTGCTTGC GCTCCCTTGG CTATGACCTG
    CCCATGGTTG AGGAAGGAGA GCCTGACCCC GAATTTGAGT CTATTCTTGA CACTGTTGAT
    CCCAACAGGG ATGGCCACGT CTCGCTGCAG GAGTACATGG CGTTCATGAT CAGCAGGGAA
    ACAGAGAACG TGAAATCCAG CGAGGAGATT GAGAGTGCTT TCCGTGCCCT CAGCTCGGAG
    GGGAAGCCTT ACGTGACCAA GGAGGAGCTC TACCAGAACC TGACCCGGGA GCAGGCCGAC
    TACTGCATTT CTCACATGAA GCCCTACATG GATGGCAAGG GCAGAGAACT TCCTTCTGCC
    TACGACTACA TAGAATTTAC ACGTTCACTC TTTGTGAATT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa43311
    Clone ID Related Accession (Same CDS sequence) XM_015279285.2 , XM_015279285.1
    Accession Version XM_015279285.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7542bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
    TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG
    TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC
    TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT
    GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT
    GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG
    ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAT CTTTCACGCC TTACAGGACT
    GCACAGCGCC CAATAGTAAC AGCCACCCAA GTTGGGTCCC ACATACGAAC TCGTCTACAG
    GAGCTGCACC GACTATGGGA GTTACTGCTG GAAAAAATGA GAGAGAAGGG AGTGAAGCTG
    TTGCAAGCAC AGAAGCTGGT GCAATTCTTA CGGGAATGTG AAGATGTCAT GGACTGGATC
    AATGACAAGG AAGCAATAGT GACCTCAGAA GAGCTTGGAC AGGACTTAGA GCATGTTGAG
    GTTTTGCAGA AGAAATTTGA AGAGTTCCAA ACAGATCTGG CAGCTCATGA GGAGAGAGTG
    AATGAAGTGA ACCAGTTCGC TGGCAAACTT ATCCAGGAAC AGCACCCTGA GGAGGAACTT
    ATAAAGTCCA AACAGGATGA AGTAAATGCA AGCTGGCAGC GTCTTAAGGG GCTTGCCCAA
    CAGAGGCAAG GAAAACTCTT TGGGGCAGCT GAAGTTCAGC GCTTCAACAG GGATGTGGAT
    GAAACTATCA GCTGGATTAA GGAGAAAGGG CAGTTGATGG CCTCAGATGA CTTTGGCAGA
    GACTTGGCCA GCGTGCAAGC TTTACTACGT AAGCATGAAG GCCTGGAAAG AGATCTTGCA
    GCTTTGGAGG ATAAGGTTAA GGCCCTCTGT GCAGAAGCTG ACCGTTTGCA GCAGTCTCAC
    CCTATAAATG CTTCTCAAAT TCAAGTGAAA CGGGAGGAAC TGATTGCCAA CTGGGAGCAG
    ATCCGAACTC TGGCAGCAGA GAGGCATGCT CGCCTTAATG ACTCCTACAG GTTGCAGCGC
    TTTCTTGCAG ATTTCCGGGA CCTCACTAGC TGGGTAACTG AGATGAAGGC TCTGATAAAT
    GCTGATGAGC TTGCCAATGA TGTGGCTGGA GCAGAAGCCC TTCTAGATAG ACATCAGGAA
    CATAAGGGAG AAATTGATGC TCATGAGGAT AGCTTCAGAT CTGCTGATGA GTCTGGGCAG
    GCTTTGCTTG CAGCTGGGCA CTATGCTTCT GATGAAGTTA AAGAAAAGCT GACTATCCTC
    TCAGATGAAA GATCAGCCTT GCTGGAACTA TGGGAGCTTC GCAGGCAACA GTATGAGCAG
    TGCATGGATC TGCAGCTTTT CTACAGAGAT ACTGAACAAG TTGACAACTG GATGAGCAAA
    CAAGAAGCTT TTCTGCTGAA CGAAGACCTT GGTGATTCTC TGGATAGCGT GGAGGCTCTT
    CTAAAGAAGC ATGAAGATTT TGAGAAATCC CTAAGTGCTC AAGAGGAGAA GATCACAGCA
    CTGGATGAGT TTGCTACTAA ACTGATTCAA AATAACCATT ATGCCATGGA TGACGTTGCT
    ACGCGCAGAG ATGCTCTTCT GAGTCGCCGA AATGCTCTTC ATGAAAGAGC CATGAAGCGC
    CGTGCCCAAC TGGCAGACTC TTTCCATCTC CAGCAGTTTT TCAGGGACTC TGATGAACTG
    AAGAGTTGGG TTAATGAAAA AATGAAAACT GCTACCGATG AGGCTTACAA GGATCCATCC
    AACTTGCAAG GTAAAGTTCA GAAGCATCAG GCTTTTGAAG CAGAGCTTTC TGCTAATCAG
    AGTCGTATTG ATGCACTGGA GAAAGCTGGC CAGAAGTTGA TTGATGTCAA TCACTATGCA
    TCTGATGAGG TGGCAGCTCG CATGAATGAA GTCATCAGCT TGTGGAAGAA ACTTCTGGAA
    GCCACCGAGC TCAAAGGTAT AAAGCTGCGT GAAGCCAATC AACAGCAGCA ATTTAATCGC
    AATGTAGAAG ACATTGAGTT GTGGCTGTAT GAAGTGGAGG GTCACTTGGC TTCTGATGAT
    TATGGAAAAG ATCTTACCAG CGTTCAGAAT CTTCAGAAGA AACATGCCCT GCTAGAGGCA
    GATGTTGCTG CCCATCAGGA TCGGATAGAT GGCATTACCA TCCAGGCACG CCAGTTCCAA
    GATGCTGGGC ACTTTGATGC TGACAATATC AAGAAGAAAC AAGAAGCTTT AGTAGCTCGT
    TATGAAGCTC TGAAGGATCC TATGGTGGCT CGCAAGCAGA AACTTGCAGA TTCTCTTCGC
    CTGCAGCAGC TTTTCCGTGA CATCGAGGAT GAAGAGACCT GGATCAGGGA AAAGGAACCT
    ATTGCAGCCT CAACAAACCG AGGCAAGGAC TTAATTGGTG TCCAGAATCT GCTAAAGAAG
    CACCAGGCTT TGCAGGCAGA AATTGCAGGC CATGAGCCTC GCATTAAAGC AGTCACACAA
    AAAGGAAATG CGATGGTGGA AGAGGGACAT TTTGCTGCTG AAGATGTGAA GATCAAATTG
    AATGAACTAA ACCAAAAATG GGACTCTCTG AAAGCCAAGG CATCTCAACG GCGACAGGAT
    CTAGAGGATT CTCTGCAAGC TCAGCAGTAT TTTGCTGATG CTAATGAGGC AGAATCATGG
    ATGAGGGAAA AGGAGCCCAT TGTAGGCAGC ACGGATTATG GAAAGGATGA AGACTCTGCT
    GAGGCTCTCC TGAAGAAGCA TGAAGCTTTG ATGTCTGATC TTTCTGCTTA CGGCAGTAGC
    ATACAGGCAT TAAGGGAACA GGCCCAGTCC TGCAGGCAAC AAGTTGCTCC CACTGATGAT
    GAAACTGGAA AAGAGCTTGT GCTAGCACTC TATGATTACC AAGAGAAGAG TCCTCGGGAG
    GTGACTATGA AGAAAGGAGA TATTCTAACC CTGCTCAACA GCACCAACAA GGACTGGTGG
    AAGGTTGAAG TTAACGATCG TCAGGGCTTT GTACCAGCTG CCTATGTGAA AAAACTAGAT
    CCTGCCCAGT CTGCATCCCG AGAGAATCTT CTAGAAGAGC AAGGCAGCAT AGCATTGCGA
    CAGGAGCAAA TTGACAACCA GTATCATTCC CTTCTTGAGC TGGGAGAAAA ACGTAAAGGC
    ATGCTAGAAA AAAGCTGCAA GAAGTTTATG CTTTTCCGTG AGGCTAATGA GCTTCAACAG
    TGGATCAATG AGAAGGAAGC TGCGCTCACT AATGAGGAAG TGGGTGCTGA TTTGGAGCAG
    GTGGAGGTGC TACAGAAGAA GTTTGATGAT TTTCAGAAGG ATTTAAAAGC TAATGAGTCA
    CGACTGAAGG ACATAAACAA GGTTGCAAAT GACCTGGAGT CTGAAGGGCT GATGGCAGAG
    GAAGTGCAAG CAGTAGAGCA CCAGGAAGTC TATGGAATGA TGCCCAGAGA TGAAACTGAT
    TCTAAGACAG CCTCTCCTTG GAAGTCTGCA CGTATGATGG TACACACAGT GGCAACGTTT
    AACTCAATCA AGGAACTGAA TGAACGCTGG AGATCCCTGC AGCAGTTGGC AGAGGAGCGA
    AGCCAGCTGT TGGGCAGCGC TCATGAAGTC CAGAGGTTCC ACAGAGATGC TGATGAAACA
    AAAGAATGGA TAGAAGAGAA GAATCAAGCA TTAAATACCG ACAACTATGG ACATGACTTG
    GCCAGTGTGC AGGCTCTGCA GCGCAAGCAT GAAGGCTTTG AGAGAGACTT GGCAGCTCTT
    GGAGACAAGG TGAATTCTCT TGGTGAAACT GCCCAGCGTC TGATCCAGTC ACATCCAGAA
    TCAGCTGAAG ATCTTCAAGA AAAATGTACT GAGTTGAACC AAGCCTGGAG TAGTCTGGGA
    AAACGTGCTG ACCAACGCAA AGAGAAGCTT GGAGATTCTC ATGACCTGCA GCGTTTCCTC
    AGTGATTTTA GAGACCTTAT GTCTTGGATC AATGGAATCC GGGGTCTGGT CTCCTCAGAT
    GAACTTGCAA AAGATGTGAC TGGAGCTGAA GCTCTGTTGG AAAGGCATCA GGAGCACCGT
    ACTGAAATAG ATGCACGGGC TGGCACTTTT CAGGCATTTG AACAGTTTGG ACAGCAGCTT
    CTGGCTCGTG GACACTATGC CAGCCCAGAG ATTAAGGAGA AACTGGATAT TCTAGATCAA
    GAACGGACAG ACCTAGAGAA GGCCTGGGTC CAGCGCAGAA TGATGCTAGA CCAGTGCTTA
    GAACTACAGC TGTTTCATCG GGATTGTGAA CAAGCTGAAA ACTGGATGGC TGCCCGAGAG
    GCGTTCCTAA ATACAGAAGA CAAAGGAGAC TCCTTAGACA GCGTGGAGGC ACTCATCAAG
    AAGCATGAAG ATTTCGATAA AGCAATCAAT GTCCAGGAAG AGAAAATTGC TGTCTTGCAG
    TCTTTTGCTG ACCAACTGAT TGCTGCTGAT CATTATGCAA AAGGAGTCAT TGCTAACAGA
    CGCAATGAGG TTCTGGACAG GTGGCGTCGT CTGAAGGCTC AGATGATTGA GAAGAGATCT
    AAGCTGGGAG AATCTCAGAC CCTCCAACAG TTCAGTCGTG ATGTTGATGA AATAGAAGCT
    TGGATCAGTG AAAAGCTTCA AACTGCAAGT GATGAGTCAT ATAAGGATCC CACAAACATC
    CAGCTTTCCA AACTGCTGAG CAAGCACCAG AAGCACCAAG CCTTTGAAGC TGAGCTCCAC
    GCCAACGCAG ATCGGATTCG TGGAGTCATT GAAATGGGGA ACTCTCTTAT TGAAAGAGGA
    GCGTGTGCTG GCAGCGAGGA TGCTGTGAAG GCTCGGCTGG CTGCCCTGGC TGACCAATGG
    GAGTTCCTGG TCCAGAAGTC ATCAGAGAAG AGTCAGAAAC TGAAAGAAGC AAATAAACAG
    CAGAATTTCA ATACCGGCAT CAAGGACTTT GATTTCTGGC TTTCAGAGGT GGAAGCTTTG
    TTGGCATCTG AAGACTATGG GAAGGACTTG GCATCAGTGA ACAACCTTCT GAAGAAGCAC
    CAATTACTGG AAGCTGATAT ATCTGCTCAT GAGGATCGGC TGAAGGACCT GAACAGCCAG
    GCTGACAGTT TGATGACCAG CAGTGCTTTC GATACCTCCC AAGTAAAGGA TAAACGTGAA
    ACTATAAATG GGCGCTTCCA GAGAATCAAG AGCATGGCAG CTGCCCGCCG TGCGAAGCTC
    AACGAGTCGC ACCGCTTGCA TCAGTTCTTC CGTGACATGG ATGATGAGGA GTCCTGGATC
    AAAGAGAAGA AACTGTTGGT GAGCTCAGAG GACTATGGCA GAGACCTGAC TGGTGTGCAG
    AACCTGAGGA AGAAACATAA GCGCTTGGAA GCAGAATTAG CTGCCCATGA ACCTGCTATC
    CAGGGTGTTC TAGACACTGG CAAGAAGCTT TCAGATGATA ACACAATTGG GAAGGAGGAG
    ATACAGCAAA GACTGGCTCA GTTTGTGGAC CACTGGAAAG AGTTAAAACA GCTGGCAGCT
    GCTCGGGGGC AGCGTCTGGA GGAGTCCCTG GAGTACCAAC AGTTTGTAGC AAATGTTGAG
    GAAGAGGAGG CATGGATCAA CGAGAAAATG ACATTGGTAG CCAGTGAGGA TTATGGAGAC
    ACACTTGCTG CTATCCAGGG CTTGCTGAAG AAGCATGAAG CATTCGAGAC TGACTTTACT
    GTCCACAAAG ACAGAGTGAA TGATGTTTGC GCTAATGGAG AGGATCTCAT TAAAAAGAAT
    AATCACCATG TGGAGAACAT TACTGCTAAG ATGAAGGGCC TCAAGGGCAA GGTATCAGAT
    CTGGAGAAAG CCGCAGCTCA GAGGAAAGCC AAACTGGATG AGAACTCTGC CTTCCTCCAG
    TTCAACTGGA AAGCAGATGT GGTGGAGTCA TGGATAGGTG AGAAGGAAAA CAGTCTGAAG
    ACAGATGATT ATGGCCGTGA CCTCTCCTCT GTGCAAACAC TACTCACCAA ACAGGAAACC
    TTTGATGCTG GACTTCAGGC TTTCCAGCAG GAGGGAATTG CAAACATCAC TGCTCTGAAA
    GACCAGCTAC TGGCAGCCAA ACATATCCAA TCAAAGGCCA TTGAGGTTCG TCACGCTTCC
    TTGATGAAAC GCTGGAATCA GCTGCTTGCT AATTCTGCAG CCAGGAAAAA GAAACTCTTG
    GAGGCTCAGG AGCACTTCAG AAAGGTTGAA GATCTCTTCT TGACTTTTGC CAAGAAGGCC
    TCTGCCTTCA ACAGTTGGTT TGAGAATGCT GAGGAGGACC TGACGGATCC CGTGCGCTGC
    AATTCCCTGG AAGAAATCAA AGCACTGCGA GAAGCCCACG ATGCGTTCCG TTCCTCACTT
    AGCTCTGCCC AAGCTGACTT CAACCAGCTG GCAGAGCTTG ATCGGCAGAT CAAGAGCTTC
    CGTGTAGCCT CCAACCCGTA CACTTGGTTT ACTATGGAGG CTCTTGAAGA AACTTGGAGG
    AATCTGCAGA AAATTATCAA GGAGCGTGAA TTGGAGTTGC AGAAGGAGCA GCGGAGGCAG
    GAAGAAAATG ACAAGTTGCG CCAGGAGTTT GCTCAGCATG CTAATGCCTT CCACCAGTGG
    ATTCAGGAGA CAAGGACTTA CCTGCTAGAT GGCATAGCCT ATCGTCGTGT CATTCGTGTC
    TATCAGTATG AAGTTGGGGA TGATCTATCT GGAAGGTCCT GCATGGTGGA GGAGTCGGGA
    ACACTGGAAT CCCAGCTGGA AGCTACTAAA CGCAAGCACC AGGAGATCCG GGCTATGAGG
    AGCCAGCTGA AGAAGATTGA GGACCTTGGA GCAGCCATGG AAGAGGCACT TATCTTGGAC
    AACAAATACA CAGAGCACAG CACCGTGGGG CTGGCGCAGC AGTGGGACCA GCTTGACCAG
    CTGGGAATGA GAATGCAGCA CAATCTGGAA CAGCAGATTC AAGCTCGAAA CACAACTGGA
    GTCACAGAGG AGGCCCTGAA GGAGTTCAGC ATGATGTTCA AGCACTTTGA CAAGGACAAA
    TCTGGACGTC TTAATCACCA GGAGTTTAAG TCTTGCTTGC GCTCCCTTGG CTATGACCTG
    CCCATGGTTG AGGAAGGAGA GCCTGACCCC GAATTTGAGT CTATTCTTGA CACTGTTGAT
    CCCAACAGGG ATGGCCACGT CTCGCTGCAG GAGTACATGG CGTTCATGAT CAGCAGGGAA
    ACAGAGAACG TGAAATCCAG CGAGGAGATT GAGAGTGCTT TCCGTGCCCT CAGCTCGGAG
    GGGAAGCCTT ACGTGACCAA GGAGGAGCTC TACCAGAACC TGACCCGGGA GCAGGCCGAC
    TACTGCATTT CTCACATGAA GCCCTACATG GATGGCAAGG GCAGAGAACT TCCTTCTGCC
    TACGACTACA TAGAATTTAC ACGTTCACTC TTTGTGAATT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015134771.1
    CDS17..7558
    Translation

    Target ORF information:

    RefSeq Version XM_015279285.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015279285.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
    TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG
    TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC
    TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT
    GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT
    GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG
    ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAT CTTTCACGCC TTACAGGACT
    GCACAGCGCC CAATAGTAAC AGCCACCCAA GTTGGGTCCC ACATACGAAC TCGTCTACAG
    GAGCTGCACC GACTATGGGA GTTACTGCTG GAAAAAATGA GAGAGAAGGG AGTGAAGCTG
    TTGCAAGCAC AGAAGCTGGT GCAATTCTTA CGGGAATGTG AAGATGTCAT GGACTGGATC
    AATGACAAGG AAGCAATAGT GACCTCAGAA GAGCTTGGAC AGGACTTAGA GCATGTTGAG
    GTTTTGCAGA AGAAATTTGA AGAGTTCCAA ACAGATCTGG CAGCTCATGA GGAGAGAGTG
    AATGAAGTGA ACCAGTTCGC TGGCAAACTT ATCCAGGAAC AGCACCCTGA GGAGGAACTT
    ATAAAGTCCA AACAGGATGA AGTAAATGCA AGCTGGCAGC GTCTTAAGGG GCTTGCCCAA
    CAGAGGCAAG GAAAACTCTT TGGGGCAGCT GAAGTTCAGC GCTTCAACAG GGATGTGGAT
    GAAACTATCA GCTGGATTAA GGAGAAAGGG CAGTTGATGG CCTCAGATGA CTTTGGCAGA
    GACTTGGCCA GCGTGCAAGC TTTACTACGT AAGCATGAAG GCCTGGAAAG AGATCTTGCA
    GCTTTGGAGG ATAAGGTTAA GGCCCTCTGT GCAGAAGCTG ACCGTTTGCA GCAGTCTCAC
    CCTATAAATG CTTCTCAAAT TCAAGTGAAA CGGGAGGAAC TGATTGCCAA CTGGGAGCAG
    ATCCGAACTC TGGCAGCAGA GAGGCATGCT CGCCTTAATG ACTCCTACAG GTTGCAGCGC
    TTTCTTGCAG ATTTCCGGGA CCTCACTAGC TGGGTAACTG AGATGAAGGC TCTGATAAAT
    GCTGATGAGC TTGCCAATGA TGTGGCTGGA GCAGAAGCCC TTCTAGATAG ACATCAGGAA
    CATAAGGGAG AAATTGATGC TCATGAGGAT AGCTTCAGAT CTGCTGATGA GTCTGGGCAG
    GCTTTGCTTG CAGCTGGGCA CTATGCTTCT GATGAAGTTA AAGAAAAGCT GACTATCCTC
    TCAGATGAAA GATCAGCCTT GCTGGAACTA TGGGAGCTTC GCAGGCAACA GTATGAGCAG
    TGCATGGATC TGCAGCTTTT CTACAGAGAT ACTGAACAAG TTGACAACTG GATGAGCAAA
    CAAGAAGCTT TTCTGCTGAA CGAAGACCTT GGTGATTCTC TGGATAGCGT GGAGGCTCTT
    CTAAAGAAGC ATGAAGATTT TGAGAAATCC CTAAGTGCTC AAGAGGAGAA GATCACAGCA
    CTGGATGAGT TTGCTACTAA ACTGATTCAA AATAACCATT ATGCCATGGA TGACGTTGCT
    ACGCGCAGAG ATGCTCTTCT GAGTCGCCGA AATGCTCTTC ATGAAAGAGC CATGAAGCGC
    CGTGCCCAAC TGGCAGACTC TTTCCATCTC CAGCAGTTTT TCAGGGACTC TGATGAACTG
    AAGAGTTGGG TTAATGAAAA AATGAAAACT GCTACCGATG AGGCTTACAA GGATCCATCC
    AACTTGCAAG GTAAAGTTCA GAAGCATCAG GCTTTTGAAG CAGAGCTTTC TGCTAATCAG
    AGTCGTATTG ATGCACTGGA GAAAGCTGGC CAGAAGTTGA TTGATGTCAA TCACTATGCA
    TCTGATGAGG TGGCAGCTCG CATGAATGAA GTCATCAGCT TGTGGAAGAA ACTTCTGGAA
    GCCACCGAGC TCAAAGGTAT AAAGCTGCGT GAAGCCAATC AACAGCAGCA ATTTAATCGC
    AATGTAGAAG ACATTGAGTT GTGGCTGTAT GAAGTGGAGG GTCACTTGGC TTCTGATGAT
    TATGGAAAAG ATCTTACCAG CGTTCAGAAT CTTCAGAAGA AACATGCCCT GCTAGAGGCA
    GATGTTGCTG CCCATCAGGA TCGGATAGAT GGCATTACCA TCCAGGCACG CCAGTTCCAA
    GATGCTGGGC ACTTTGATGC TGACAATATC AAGAAGAAAC AAGAAGCTTT AGTAGCTCGT
    TATGAAGCTC TGAAGGATCC TATGGTGGCT CGCAAGCAGA AACTTGCAGA TTCTCTTCGC
    CTGCAGCAGC TTTTCCGTGA CATCGAGGAT GAAGAGACCT GGATCAGGGA AAAGGAACCT
    ATTGCAGCCT CAACAAACCG AGGCAAGGAC TTAATTGGTG TCCAGAATCT GCTAAAGAAG
    CACCAGGCTT TGCAGGCAGA AATTGCAGGC CATGAGCCTC GCATTAAAGC AGTCACACAA
    AAAGGAAATG CGATGGTGGA AGAGGGACAT TTTGCTGCTG AAGATGTGAA GATCAAATTG
    AATGAACTAA ACCAAAAATG GGACTCTCTG AAAGCCAAGG CATCTCAACG GCGACAGGAT
    CTAGAGGATT CTCTGCAAGC TCAGCAGTAT TTTGCTGATG CTAATGAGGC AGAATCATGG
    ATGAGGGAAA AGGAGCCCAT TGTAGGCAGC ACGGATTATG GAAAGGATGA AGACTCTGCT
    GAGGCTCTCC TGAAGAAGCA TGAAGCTTTG ATGTCTGATC TTTCTGCTTA CGGCAGTAGC
    ATACAGGCAT TAAGGGAACA GGCCCAGTCC TGCAGGCAAC AAGTTGCTCC CACTGATGAT
    GAAACTGGAA AAGAGCTTGT GCTAGCACTC TATGATTACC AAGAGAAGAG TCCTCGGGAG
    GTGACTATGA AGAAAGGAGA TATTCTAACC CTGCTCAACA GCACCAACAA GGACTGGTGG
    AAGGTTGAAG TTAACGATCG TCAGGGCTTT GTACCAGCTG CCTATGTGAA AAAACTAGAT
    CCTGCCCAGT CTGCATCCCG AGAGAATCTT CTAGAAGAGC AAGGCAGCAT AGCATTGCGA
    CAGGAGCAAA TTGACAACCA GTATCATTCC CTTCTTGAGC TGGGAGAAAA ACGTAAAGGC
    ATGCTAGAAA AAAGCTGCAA GAAGTTTATG CTTTTCCGTG AGGCTAATGA GCTTCAACAG
    TGGATCAATG AGAAGGAAGC TGCGCTCACT AATGAGGAAG TGGGTGCTGA TTTGGAGCAG
    GTGGAGGTGC TACAGAAGAA GTTTGATGAT TTTCAGAAGG ATTTAAAAGC TAATGAGTCA
    CGACTGAAGG ACATAAACAA GGTTGCAAAT GACCTGGAGT CTGAAGGGCT GATGGCAGAG
    GAAGTGCAAG CAGTAGAGCA CCAGGAAGTC TATGGAATGA TGCCCAGAGA TGAAACTGAT
    TCTAAGACAG CCTCTCCTTG GAAGTCTGCA CGTATGATGG TACACACAGT GGCAACGTTT
    AACTCAATCA AGGAACTGAA TGAACGCTGG AGATCCCTGC AGCAGTTGGC AGAGGAGCGA
    AGCCAGCTGT TGGGCAGCGC TCATGAAGTC CAGAGGTTCC ACAGAGATGC TGATGAAACA
    AAAGAATGGA TAGAAGAGAA GAATCAAGCA TTAAATACCG ACAACTATGG ACATGACTTG
    GCCAGTGTGC AGGCTCTGCA GCGCAAGCAT GAAGGCTTTG AGAGAGACTT GGCAGCTCTT
    GGAGACAAGG TGAATTCTCT TGGTGAAACT GCCCAGCGTC TGATCCAGTC ACATCCAGAA
    TCAGCTGAAG ATCTTCAAGA AAAATGTACT GAGTTGAACC AAGCCTGGAG TAGTCTGGGA
    AAACGTGCTG ACCAACGCAA AGAGAAGCTT GGAGATTCTC ATGACCTGCA GCGTTTCCTC
    AGTGATTTTA GAGACCTTAT GTCTTGGATC AATGGAATCC GGGGTCTGGT CTCCTCAGAT
    GAACTTGCAA AAGATGTGAC TGGAGCTGAA GCTCTGTTGG AAAGGCATCA GGAGCACCGT
    ACTGAAATAG ATGCACGGGC TGGCACTTTT CAGGCATTTG AACAGTTTGG ACAGCAGCTT
    CTGGCTCGTG GACACTATGC CAGCCCAGAG ATTAAGGAGA AACTGGATAT TCTAGATCAA
    GAACGGACAG ACCTAGAGAA GGCCTGGGTC CAGCGCAGAA TGATGCTAGA CCAGTGCTTA
    GAACTACAGC TGTTTCATCG GGATTGTGAA CAAGCTGAAA ACTGGATGGC TGCCCGAGAG
    GCGTTCCTAA ATACAGAAGA CAAAGGAGAC TCCTTAGACA GCGTGGAGGC ACTCATCAAG
    AAGCATGAAG ATTTCGATAA AGCAATCAAT GTCCAGGAAG AGAAAATTGC TGTCTTGCAG
    TCTTTTGCTG ACCAACTGAT TGCTGCTGAT CATTATGCAA AAGGAGTCAT TGCTAACAGA
    CGCAATGAGG TTCTGGACAG GTGGCGTCGT CTGAAGGCTC AGATGATTGA GAAGAGATCT
    AAGCTGGGAG AATCTCAGAC CCTCCAACAG TTCAGTCGTG ATGTTGATGA AATAGAAGCT
    TGGATCAGTG AAAAGCTTCA AACTGCAAGT GATGAGTCAT ATAAGGATCC CACAAACATC
    CAGCTTTCCA AACTGCTGAG CAAGCACCAG AAGCACCAAG CCTTTGAAGC TGAGCTCCAC
    GCCAACGCAG ATCGGATTCG TGGAGTCATT GAAATGGGGA ACTCTCTTAT TGAAAGAGGA
    GCGTGTGCTG GCAGCGAGGA TGCTGTGAAG GCTCGGCTGG CTGCCCTGGC TGACCAATGG
    GAGTTCCTGG TCCAGAAGTC ATCAGAGAAG AGTCAGAAAC TGAAAGAAGC AAATAAACAG
    CAGAATTTCA ATACCGGCAT CAAGGACTTT GATTTCTGGC TTTCAGAGGT GGAAGCTTTG
    TTGGCATCTG AAGACTATGG GAAGGACTTG GCATCAGTGA ACAACCTTCT GAAGAAGCAC
    CAATTACTGG AAGCTGATAT ATCTGCTCAT GAGGATCGGC TGAAGGACCT GAACAGCCAG
    GCTGACAGTT TGATGACCAG CAGTGCTTTC GATACCTCCC AAGTAAAGGA TAAACGTGAA
    ACTATAAATG GGCGCTTCCA GAGAATCAAG AGCATGGCAG CTGCCCGCCG TGCGAAGCTC
    AACGAGTCGC ACCGCTTGCA TCAGTTCTTC CGTGACATGG ATGATGAGGA GTCCTGGATC
    AAAGAGAAGA AACTGTTGGT GAGCTCAGAG GACTATGGCA GAGACCTGAC TGGTGTGCAG
    AACCTGAGGA AGAAACATAA GCGCTTGGAA GCAGAATTAG CTGCCCATGA ACCTGCTATC
    CAGGGTGTTC TAGACACTGG CAAGAAGCTT TCAGATGATA ACACAATTGG GAAGGAGGAG
    ATACAGCAAA GACTGGCTCA GTTTGTGGAC CACTGGAAAG AGTTAAAACA GCTGGCAGCT
    GCTCGGGGGC AGCGTCTGGA GGAGTCCCTG GAGTACCAAC AGTTTGTAGC AAATGTTGAG
    GAAGAGGAGG CATGGATCAA CGAGAAAATG ACATTGGTAG CCAGTGAGGA TTATGGAGAC
    ACACTTGCTG CTATCCAGGG CTTGCTGAAG AAGCATGAAG CATTCGAGAC TGACTTTACT
    GTCCACAAAG ACAGAGTGAA TGATGTTTGC GCTAATGGAG AGGATCTCAT TAAAAAGAAT
    AATCACCATG TGGAGAACAT TACTGCTAAG ATGAAGGGCC TCAAGGGCAA GGTATCAGAT
    CTGGAGAAAG CCGCAGCTCA GAGGAAAGCC AAACTGGATG AGAACTCTGC CTTCCTCCAG
    TTCAACTGGA AAGCAGATGT GGTGGAGTCA TGGATAGGTG AGAAGGAAAA CAGTCTGAAG
    ACAGATGATT ATGGCCGTGA CCTCTCCTCT GTGCAAACAC TACTCACCAA ACAGGAAACC
    TTTGATGCTG GACTTCAGGC TTTCCAGCAG GAGGGAATTG CAAACATCAC TGCTCTGAAA
    GACCAGCTAC TGGCAGCCAA ACATATCCAA TCAAAGGCCA TTGAGGTTCG TCACGCTTCC
    TTGATGAAAC GCTGGAATCA GCTGCTTGCT AATTCTGCAG CCAGGAAAAA GAAACTCTTG
    GAGGCTCAGG AGCACTTCAG AAAGGTTGAA GATCTCTTCT TGACTTTTGC CAAGAAGGCC
    TCTGCCTTCA ACAGTTGGTT TGAGAATGCT GAGGAGGACC TGACGGATCC CGTGCGCTGC
    AATTCCCTGG AAGAAATCAA AGCACTGCGA GAAGCCCACG ATGCGTTCCG TTCCTCACTT
    AGCTCTGCCC AAGCTGACTT CAACCAGCTG GCAGAGCTTG ATCGGCAGAT CAAGAGCTTC
    CGTGTAGCCT CCAACCCGTA CACTTGGTTT ACTATGGAGG CTCTTGAAGA AACTTGGAGG
    AATCTGCAGA AAATTATCAA GGAGCGTGAA TTGGAGTTGC AGAAGGAGCA GCGGAGGCAG
    GAAGAAAATG ACAAGTTGCG CCAGGAGTTT GCTCAGCATG CTAATGCCTT CCACCAGTGG
    ATTCAGGAGA CAAGGACTTA CCTGCTAGAT GGCATAGCCT ATCGTCGTGT CATTCGTGTC
    TATCAGTATG AAGTTGGGGA TGATCTATCT GGAAGGTCCT GCATGGTGGA GGAGTCGGGA
    ACACTGGAAT CCCAGCTGGA AGCTACTAAA CGCAAGCACC AGGAGATCCG GGCTATGAGG
    AGCCAGCTGA AGAAGATTGA GGACCTTGGA GCAGCCATGG AAGAGGCACT TATCTTGGAC
    AACAAATACA CAGAGCACAG CACCGTGGGG CTGGCGCAGC AGTGGGACCA GCTTGACCAG
    CTGGGAATGA GAATGCAGCA CAATCTGGAA CAGCAGATTC AAGCTCGAAA CACAACTGGA
    GTCACAGAGG AGGCCCTGAA GGAGTTCAGC ATGATGTTCA AGCACTTTGA CAAGGACAAA
    TCTGGACGTC TTAATCACCA GGAGTTTAAG TCTTGCTTGC GCTCCCTTGG CTATGACCTG
    CCCATGGTTG AGGAAGGAGA GCCTGACCCC GAATTTGAGT CTATTCTTGA CACTGTTGAT
    CCCAACAGGG ATGGCCACGT CTCGCTGCAG GAGTACATGG CGTTCATGAT CAGCAGGGAA
    ACAGAGAACG TGAAATCCAG CGAGGAGATT GAGAGTGCTT TCCGTGCCCT CAGCTCGGAG
    GGGAAGCCTT ACGTGACCAA GGAGGAGCTC TACCAGAACC TGACCCGGGA GCAGGCCGAC
    TACTGCATTT CTCACATGAA GCCCTACATG GATGGCAAGG GCAGAGAACT TCCTTCTGCC
    TACGACTACA TAGAATTTAC ACGTTCACTC TTTGTGAATT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa43312
    Clone ID Related Accession (Same CDS sequence) XM_015279286.1 , XM_015279286.2
    Accession Version XM_015279286.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7539bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
    TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG
    TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC
    TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT
    GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT
    GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG
    ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAT CTTTCACGCC TTACAGGACT
    GCACAGCGCC CAATAGTAAC AGCCACCCAA GTTGGGTCCC ACATACGAAC TCGTCTACAG
    GAGCTGCACC GACTATGGGA GTTACTGCTG GAAAAAATGA GAGAGAAGGG AGTGAAGCTG
    TTGCAAGCAC AGAAGCTGGT GCAATTCTTA CGGGAATGTG AAGATGTCAT GGACTGGATC
    AATGACAAGG AAGCAATAGT GACCTCAGAA GAGCTTGGAC AGGACTTAGA GCATGTTGAG
    GTTTTGCAGA AGAAATTTGA AGAGTTCCAA ACAGATCTGG CAGCTCATGA GGAGAGAGTG
    AATGAAGTGA ACCAGTTCGC TGGCAAACTT ATCCAGGAAC AGCACCCTGA GGAGGAACTT
    ATAAAGTCCA AACAGGATGA AGTAAATGCA AGCTGGCAGC GTCTTAAGGG GCTTGCCCAA
    CAGAGGCAAG GAAAACTCTT TGGGGCAGCT GAAGTTCAGC GCTTCAACAG GGATGTGGAT
    GAAACTATCA GCTGGATTAA GGAGAAAGGG CAGTTGATGG CCTCAGATGA CTTTGGCAGA
    GACTTGGCCA GCGTGCAAGC TTTACTACGT AAGCATGAAG GCCTGGAAAG AGATCTTGCA
    GCTTTGGAGG ATAAGGTTAA GGCCCTCTGT GCAGAAGCTG ACCGTTTGCA GCAGTCTCAC
    CCTATAAATG CTTCTCAAAT TCAAGTGAAA CGGGAGGAAC TGATTGCCAA CTGGGAGCAG
    ATCCGAACTC TGGCAGCAGA GAGGCATGCT CGCCTTAATG ACTCCTACAG GTTGCAGCGC
    TTTCTTGCAG ATTTCCGGGA CCTCACTAGC TGGGTAACTG AGATGAAGGC TCTGATAAAT
    GCTGATGAGC TTGCCAATGA TGTGGCTGGA GCAGAAGCCC TTCTAGATAG ACATCAGGAA
    CATAAGGGAG AAATTGATGC TCATGAGGAT AGCTTCAGAT CTGCTGATGA GTCTGGGCAG
    GCTTTGCTTG CAGCTGGGCA CTATGCTTCT GATGAAGTTA AAGAAAAGCT GACTATCCTC
    TCAGATGAAA GATCAGCCTT GCTGGAACTA TGGGAGCTTC GCAGGCAACA GTATGAGCAG
    TGCATGGATC TGCAGCTTTT CTACAGAGAT ACTGAACAAG TTGACAACTG GATGAGCAAA
    CAAGAAGCTT TTCTGCTGAA CGAAGACCTT GGTGATTCTC TGGATAGCGT GGAGGCTCTT
    CTAAAGAAGC ATGAAGATTT TGAGAAATCC CTAAGTGCTC AAGAGGAGAA GATCACAGCA
    CTGGATGAGT TTGCTACTAA ACTGATTCAA AATAACCATT ATGCCATGGA TGACGTTGCT
    ACGCGCAGAG ATGCTCTTCT GAGTCGCCGA AATGCTCTTC ATGAAAGAGC CATGAAGCGC
    CGTGCCCAAC TGGCAGACTC TTTCCATCTC CAGCAGTTTT TCAGGGACTC TGATGAACTG
    AAGAGTTGGG TTAATGAAAA AATGAAAACT GCTACCGATG AGGCTTACAA GGATCCATCC
    AACTTGCAAG GTAAAGTTCA GAAGCATCAG GCTTTTGAAG CAGAGCTTTC TGCTAATCAG
    AGTCGTATTG ATGCACTGGA GAAAGCTGGC CAGAAGTTGA TTGATGTCAA TCACTATGCA
    TCTGATGAGG TGGCAGCTCG CATGAATGAA GTCATCAGCT TGTGGAAGAA ACTTCTGGAA
    GCCACCGAGC TCAAAGGTAT AAAGCTGCGT GAAGCCAATC AACAGCAGCA ATTTAATCGC
    AATGTAGAAG ACATTGAGTT GTGGCTGTAT GAAGTGGAGG GTCACTTGGC TTCTGATGAT
    TATGGAAAAG ATCTTACCAG CGTTCAGAAT CTTCAGAAGA AACATGCCCT GCTAGAGGCA
    GATGTTGCTG CCCATCAGGA TCGGATAGAT GGCATTACCA TCCAGGCACG CCAGTTCCAA
    GATGCTGGGC ACTTTGATGC TGACAATATC AAGAAGAAAC AAGAAGCTTT AGTAGCTCGT
    TATGAAGCTC TGAAGGATCC TATGGTGGCT CGCAAGCAGA AACTTGCAGA TTCTCTTCGC
    CTGCAGCAGC TTTTCCGTGA CATCGAGGAT GAAGAGACCT GGATCAGGGA AAAGGAACCT
    ATTGCAGCCT CAACAAACCG AGGCAAGGAC TTAATTGGTG TCCAGAATCT GCTAAAGAAG
    CACCAGGCTT TGCAGGCAGA AATTGCAGGC CATGAGCCTC GCATTAAAGC AGTCACACAA
    AAAGGAAATG CGATGGTGGA AGAGGGACAT TTTGCTGCTG AAGATGTGAA GATCAAATTG
    AATGAACTAA ACCAAAAATG GGACTCTCTG AAAGCCAAGG CATCTCAACG GCGACAGGAT
    CTAGAGGATT CTCTGCAAGC TCAGCAGTAT TTTGCTGATG CTAATGAGGC AGAATCATGG
    ATGAGGGAAA AGGAGCCCAT TGTAGGCAGC ACGGATTATG GAAAGGATGA AGACTCTGCT
    GAGGCTCTCC TGAAGAAGCA TGAAGCTTTG ATGTCTGATC TTTCTGCTTA CGGCAGTAGC
    ATACAGGCAT TAAGGGAACA GGCCCAGTCC TGCAGGCAAC AAGTTGCTCC CACTGATGAT
    GAAACTGGAA AAGAGCTTGT GCTAGCACTC TATGATTACC AAGAGAAGAG TCCTCGGGAG
    GTGACTATGA AGAAAGGAGA TATTCTAACC CTGCTCAACA GCACCAACAA GGACTGGTGG
    AAGGTTGAAG TTAACGATCG TCAGGGCTTT GTACCAGCTG CCTATGTGAA AAAACTAGAT
    CCTGCCCAGT CTGCATCCCG AGAGAATCTT CTAGAAGAGC AAGGCAGCAT AGCATTGCGA
    CAGGAGCAAA TTGACAACCA GACTCTCATT ACTAAGGAAG TCGGCAGTGT ATCTCTGCGT
    ATGAAACAGG TCGAAGAACT GTATCATTCC CTTCTTGAGC TGGGAGAAAA ACGTAAAGGC
    ATGCTAGAAA AAAGCTGCAA GAAGTTTATG CTTTTCCGTG AGGCTAATGA GCTTCAACAG
    TGGATCAATG AGAAGGAAGC TGCGCTCACT AATGAGGAAG TGGGTGCTGA TTTGGAGCAG
    GTGGAGGTGC TACAGAAGAA GTTTGATGAT TTTCAGAAGG ATTTAAAAGC TAATGAGTCA
    CGACTGAAGG ACATAAACAA GGTTGCAAAT GACCTGGAGT CTGAAGGGCT GATGGCAGAG
    GAAGTGCAAG CAGTAGAGCA CCAGGAAGTC TATGGAATGA TGCCCAGAGA TGAAACTGAT
    TCTAAGACAG CCTCTCCTTG GAAGTCTGCA CGTATGATGG TACACACAGT GGCAACGTTT
    AACTCAATCA AGGAACTGAA TGAACGCTGG AGATCCCTGC AGCAGTTGGC AGAGGAGCGA
    AGCCAGCTGT TGGGCAGCGC TCATGAAGTC CAGAGGTTCC ACAGAGATGC TGATGAAACA
    AAAGAATGGA TAGAAGAGAA GAATCAAGCA TTAAATACCG ACAACTATGG ACATGACTTG
    GCCAGTGTGC AGGCTCTGCA GCGCAAGCAT GAAGGCTTTG AGAGAGACTT GGCAGCTCTT
    GGAGACAAGG TGAATTCTCT TGGTGAAACT GCCCAGCGTC TGATCCAGTC ACATCCAGAA
    TCAGCTGAAG ATCTTCAAGA AAAATGTACT GAGTTGAACC AAGCCTGGAG TAGTCTGGGA
    AAACGTGCTG ACCAACGCAA AGAGAAGCTT GGAGATTCTC ATGACCTGCA GCGTTTCCTC
    AGTGATTTTA GAGACCTTAT GTCTTGGATC AATGGAATCC GGGGTCTGGT CTCCTCAGAT
    GAACTTGCAA AAGATGTGAC TGGAGCTGAA GCTCTGTTGG AAAGGCATCA GGAGCACCGT
    ACTGAAATAG ATGCACGGGC TGGCACTTTT CAGGCATTTG AACAGTTTGG ACAGCAGCTT
    CTGGCTCGTG GACACTATGC CAGCCCAGAG ATTAAGGAGA AACTGGATAT TCTAGATCAA
    GAACGGACAG ACCTAGAGAA GGCCTGGGTC CAGCGCAGAA TGATGCTAGA CCAGTGCTTA
    GAACTACAGC TGTTTCATCG GGATTGTGAA CAAGCTGAAA ACTGGATGGC TGCCCGAGAG
    GCGTTCCTAA ATACAGAAGA CAAAGGAGAC TCCTTAGACA GCGTGGAGGC ACTCATCAAG
    AAGCATGAAG ATTTCGATAA AGCAATCAAT GTCCAGGAAG AGAAAATTGC TGTCTTGCAG
    TCTTTTGCTG ACCAACTGAT TGCTGCTGAT CATTATGCAA AAGGAGTCAT TGCTAACAGA
    CGCAATGAGG TTCTGGACAG GTGGCGTCGT CTGAAGGCTC AGATGATTGA GAAGAGATCT
    AAGCTGGGAG AATCTCAGAC CCTCCAACAG TTCAGTCGTG ATGTTGATGA AATAGAAGCT
    TGGATCAGTG AAAAGCTTCA AACTGCAAGT GATGAGTCAT ATAAGGATCC CACAAACATC
    CAGCTTTCCA AACTGCTGAG CAAGCACCAG AAGCACCAAG CCTTTGAAGC TGAGCTCCAC
    GCCAACGCAG ATCGGATTCG TGGAGTCATT GAAATGGGGA ACTCTCTTAT TGAAAGAGGA
    GCGTGTGCTG GCAGCGAGGA TGCTGTGAAG GCTCGGCTGG CTGCCCTGGC TGACCAATGG
    GAGTTCCTGG TCCAGAAGTC ATCAGAGAAG AGTCAGAAAC TGAAAGAAGC AAATAAACAG
    CAGAATTTCA ATACCGGCAT CAAGGACTTT GATTTCTGGC TTTCAGAGGT GGAAGCTTTG
    TTGGCATCTG AAGACTATGG GAAGGACTTG GCATCAGTGA ACAACCTTCT GAAGAAGCAC
    CAATTACTGG AAGCTGATAT ATCTGCTCAT GAGGATCGGC TGAAGGACCT GAACAGCCAG
    GCTGACAGTT TGATGACCAG CAGTGCTTTC GATACCTCCC AAGTAAAGGA TAAACGTGAA
    ACTATAAATG GGCGCTTCCA GAGAATCAAG AGCATGGCAG CTGCCCGCCG TGCGAAGCTC
    AACGAGTCGC ACCGCTTGCA TCAGTTCTTC CGTGACATGG ATGATGAGGA GTCCTGGATC
    AAAGAGAAGA AACTGTTGGT GAGCTCAGAG GACTATGGCA GAGACCTGAC TGGTGTGCAG
    AACCTGAGGA AGAAACATAA GCGCTTGGAA GCAGAATTAG CTGCCCATGA ACCTGCTATC
    CAGGGTGTTC TAGACACTGG CAAGAAGCTT TCAGATGATA ACACAATTGG GAAGGAGGAG
    ATACAGCAAA GACTGGCTCA GTTTGTGGAC CACTGGAAAG AGTTAAAACA GCTGGCAGCT
    GCTCGGGGGC AGCGTCTGGA GGAGTCCCTG GAGTACCAAC AGTTTGTAGC AAATGTTGAG
    GAAGAGGAGG CATGGATCAA CGAGAAAATG ACATTGGTAG CCAGTGAGGA TTATGGAGAC
    ACACTTGCTG CTATCCAGGG CTTGCTGAAG AAGCATGAAG CATTCGAGAC TGACTTTACT
    GTCCACAAAG ACAGAGTGAA TGATGTTTGC GCTAATGGAG AGGATCTCAT TAAAAAGAAT
    AATCACCATG TGGAGAACAT TACTGCTAAG ATGAAGGGCC TCAAGGGCAA GGTATCAGAT
    CTGGAGAAAG CCGCAGCTCA GAGGAAAGCC AAACTGGATG AGAACTCTGC CTTCCTCCAG
    TTCAACTGGA AAGCAGATGT GGTGGAGTCA TGGATAGGTG AGAAGGAAAA CAGTCTGAAG
    ACAGATGATT ATGGCCGTGA CCTCTCCTCT GTGCAAACAC TACTCACCAA ACAGGAAACC
    TTTGATGCTG GACTTCAGGC TTTCCAGCAG GAGGGAATTG CAAACATCAC TGCTCTGAAA
    GACCAGCTAC TGGCAGCCAA ACATATCCAA TCAAAGGCCA TTGAGGTTCG TCACGCTTCC
    TTGATGAAAC GCTGGAATCA GCTGCTTGCT AATTCTGCAG CCAGGAAAAA GAAACTCTTG
    GAGGCTCAGG AGCACTTCAG AAAGGTTGAA GATCTCTTCT TGACTTTTGC CAAGAAGGCC
    TCTGCCTTCA ACAGTTGGTT TGAGAATGCT GAGGAGGACC TGACGGATCC CGTGCGCTGC
    AATTCCCTGG AAGAAATCAA AGCACTGCGA GAAGCCCACG ATGCGTTCCG TTCCTCACTT
    AGCTCTGCCC AAGCTGACTT CAACCAGCTG GCAGAGCTTG ATCGGCAGAT CAAGAGCTTC
    CGTGTAGCCT CCAACCCGTA CACTTGGTTT ACTATGGAGG CTCTTGAAGA AACTTGGAGG
    AATCTGCAGA AAATTATCAA GGAGCGTGAA TTGGAGTTGC AGAAGGAGCA GCGGAGGCAG
    GAAGAAAATG ACAAGTTGCG CCAGGAGTTT GCTCAGCATG CTAATGCCTT CCACCAGTGG
    ATTCAGGAGA CAAGGACTTA CCTGCTAGAT GGGTCCTGCA TGGTGGAGGA GTCGGGAACA
    CTGGAATCCC AGCTGGAAGC TACTAAACGC AAGCACCAGG AGATCCGGGC TATGAGGAGC
    CAGCTGAAGA AGATTGAGGA CCTTGGAGCA GCCATGGAAG AGGCACTTAT CTTGGACAAC
    AAATACACAG AGCACAGCAC CGTGGGGCTG GCGCAGCAGT GGGACCAGCT TGACCAGCTG
    GGAATGAGAA TGCAGCACAA TCTGGAACAG CAGATTCAAG CTCGAAACAC AACTGGAGTC
    ACAGAGGAGG CCCTGAAGGA GTTCAGCATG ATGTTCAAGC ACTTTGACAA GGACAAATCT
    GGACGTCTTA ATCACCAGGA GTTTAAGTCT TGCTTGCGCT CCCTTGGCTA TGACCTGCCC
    ATGGTTGAGG AAGGAGAGCC TGACCCCGAA TTTGAGTCTA TTCTTGACAC TGTTGATCCC
    AACAGGGATG GCCACGTCTC GCTGCAGGAG TACATGGCGT TCATGATCAG CAGGGAAACA
    GAGAACGTGA AATCCAGCGA GGAGATTGAG AGTGCTTTCC GTGCCCTCAG CTCGGAGGGG
    AAGCCTTACG TGACCAAGGA GGAGCTCTAC CAGAACCTGA CCCGGGAGCA GGCCGACTAC
    TGCATTTCTC ACATGAAGCC CTACATGGAT GGCAAGGGCA GAGAACTTCC TTCTGCCTAC
    GACTACATAG AATTTACACG TTCACTCTTT GTGAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015134772.1
    CDS13..7551
    Translation

    Target ORF information:

    RefSeq Version XM_015279286.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015279286.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
    TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG
    TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC
    TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT
    GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT
    GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG
    ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAT CTTTCACGCC TTACAGGACT
    GCACAGCGCC CAATAGTAAC AGCCACCCAA GTTGGGTCCC ACATACGAAC TCGTCTACAG
    GAGCTGCACC GACTATGGGA GTTACTGCTG GAAAAAATGA GAGAGAAGGG AGTGAAGCTG
    TTGCAAGCAC AGAAGCTGGT GCAATTCTTA CGGGAATGTG AAGATGTCAT GGACTGGATC
    AATGACAAGG AAGCAATAGT GACCTCAGAA GAGCTTGGAC AGGACTTAGA GCATGTTGAG
    GTTTTGCAGA AGAAATTTGA AGAGTTCCAA ACAGATCTGG CAGCTCATGA GGAGAGAGTG
    AATGAAGTGA ACCAGTTCGC TGGCAAACTT ATCCAGGAAC AGCACCCTGA GGAGGAACTT
    ATAAAGTCCA AACAGGATGA AGTAAATGCA AGCTGGCAGC GTCTTAAGGG GCTTGCCCAA
    CAGAGGCAAG GAAAACTCTT TGGGGCAGCT GAAGTTCAGC GCTTCAACAG GGATGTGGAT
    GAAACTATCA GCTGGATTAA GGAGAAAGGG CAGTTGATGG CCTCAGATGA CTTTGGCAGA
    GACTTGGCCA GCGTGCAAGC TTTACTACGT AAGCATGAAG GCCTGGAAAG AGATCTTGCA
    GCTTTGGAGG ATAAGGTTAA GGCCCTCTGT GCAGAAGCTG ACCGTTTGCA GCAGTCTCAC
    CCTATAAATG CTTCTCAAAT TCAAGTGAAA CGGGAGGAAC TGATTGCCAA CTGGGAGCAG
    ATCCGAACTC TGGCAGCAGA GAGGCATGCT CGCCTTAATG ACTCCTACAG GTTGCAGCGC
    TTTCTTGCAG ATTTCCGGGA CCTCACTAGC TGGGTAACTG AGATGAAGGC TCTGATAAAT
    GCTGATGAGC TTGCCAATGA TGTGGCTGGA GCAGAAGCCC TTCTAGATAG ACATCAGGAA
    CATAAGGGAG AAATTGATGC TCATGAGGAT AGCTTCAGAT CTGCTGATGA GTCTGGGCAG
    GCTTTGCTTG CAGCTGGGCA CTATGCTTCT GATGAAGTTA AAGAAAAGCT GACTATCCTC
    TCAGATGAAA GATCAGCCTT GCTGGAACTA TGGGAGCTTC GCAGGCAACA GTATGAGCAG
    TGCATGGATC TGCAGCTTTT CTACAGAGAT ACTGAACAAG TTGACAACTG GATGAGCAAA
    CAAGAAGCTT TTCTGCTGAA CGAAGACCTT GGTGATTCTC TGGATAGCGT GGAGGCTCTT
    CTAAAGAAGC ATGAAGATTT TGAGAAATCC CTAAGTGCTC AAGAGGAGAA GATCACAGCA
    CTGGATGAGT TTGCTACTAA ACTGATTCAA AATAACCATT ATGCCATGGA TGACGTTGCT
    ACGCGCAGAG ATGCTCTTCT GAGTCGCCGA AATGCTCTTC ATGAAAGAGC CATGAAGCGC
    CGTGCCCAAC TGGCAGACTC TTTCCATCTC CAGCAGTTTT TCAGGGACTC TGATGAACTG
    AAGAGTTGGG TTAATGAAAA AATGAAAACT GCTACCGATG AGGCTTACAA GGATCCATCC
    AACTTGCAAG GTAAAGTTCA GAAGCATCAG GCTTTTGAAG CAGAGCTTTC TGCTAATCAG
    AGTCGTATTG ATGCACTGGA GAAAGCTGGC CAGAAGTTGA TTGATGTCAA TCACTATGCA
    TCTGATGAGG TGGCAGCTCG CATGAATGAA GTCATCAGCT TGTGGAAGAA ACTTCTGGAA
    GCCACCGAGC TCAAAGGTAT AAAGCTGCGT GAAGCCAATC AACAGCAGCA ATTTAATCGC
    AATGTAGAAG ACATTGAGTT GTGGCTGTAT GAAGTGGAGG GTCACTTGGC TTCTGATGAT
    TATGGAAAAG ATCTTACCAG CGTTCAGAAT CTTCAGAAGA AACATGCCCT GCTAGAGGCA
    GATGTTGCTG CCCATCAGGA TCGGATAGAT GGCATTACCA TCCAGGCACG CCAGTTCCAA
    GATGCTGGGC ACTTTGATGC TGACAATATC AAGAAGAAAC AAGAAGCTTT AGTAGCTCGT
    TATGAAGCTC TGAAGGATCC TATGGTGGCT CGCAAGCAGA AACTTGCAGA TTCTCTTCGC
    CTGCAGCAGC TTTTCCGTGA CATCGAGGAT GAAGAGACCT GGATCAGGGA AAAGGAACCT
    ATTGCAGCCT CAACAAACCG AGGCAAGGAC TTAATTGGTG TCCAGAATCT GCTAAAGAAG
    CACCAGGCTT TGCAGGCAGA AATTGCAGGC CATGAGCCTC GCATTAAAGC AGTCACACAA
    AAAGGAAATG CGATGGTGGA AGAGGGACAT TTTGCTGCTG AAGATGTGAA GATCAAATTG
    AATGAACTAA ACCAAAAATG GGACTCTCTG AAAGCCAAGG CATCTCAACG GCGACAGGAT
    CTAGAGGATT CTCTGCAAGC TCAGCAGTAT TTTGCTGATG CTAATGAGGC AGAATCATGG
    ATGAGGGAAA AGGAGCCCAT TGTAGGCAGC ACGGATTATG GAAAGGATGA AGACTCTGCT
    GAGGCTCTCC TGAAGAAGCA TGAAGCTTTG ATGTCTGATC TTTCTGCTTA CGGCAGTAGC
    ATACAGGCAT TAAGGGAACA GGCCCAGTCC TGCAGGCAAC AAGTTGCTCC CACTGATGAT
    GAAACTGGAA AAGAGCTTGT GCTAGCACTC TATGATTACC AAGAGAAGAG TCCTCGGGAG
    GTGACTATGA AGAAAGGAGA TATTCTAACC CTGCTCAACA GCACCAACAA GGACTGGTGG
    AAGGTTGAAG TTAACGATCG TCAGGGCTTT GTACCAGCTG CCTATGTGAA AAAACTAGAT
    CCTGCCCAGT CTGCATCCCG AGAGAATCTT CTAGAAGAGC AAGGCAGCAT AGCATTGCGA
    CAGGAGCAAA TTGACAACCA GACTCTCATT ACTAAGGAAG TCGGCAGTGT ATCTCTGCGT
    ATGAAACAGG TCGAAGAACT GTATCATTCC CTTCTTGAGC TGGGAGAAAA ACGTAAAGGC
    ATGCTAGAAA AAAGCTGCAA GAAGTTTATG CTTTTCCGTG AGGCTAATGA GCTTCAACAG
    TGGATCAATG AGAAGGAAGC TGCGCTCACT AATGAGGAAG TGGGTGCTGA TTTGGAGCAG
    GTGGAGGTGC TACAGAAGAA GTTTGATGAT TTTCAGAAGG ATTTAAAAGC TAATGAGTCA
    CGACTGAAGG ACATAAACAA GGTTGCAAAT GACCTGGAGT CTGAAGGGCT GATGGCAGAG
    GAAGTGCAAG CAGTAGAGCA CCAGGAAGTC TATGGAATGA TGCCCAGAGA TGAAACTGAT
    TCTAAGACAG CCTCTCCTTG GAAGTCTGCA CGTATGATGG TACACACAGT GGCAACGTTT
    AACTCAATCA AGGAACTGAA TGAACGCTGG AGATCCCTGC AGCAGTTGGC AGAGGAGCGA
    AGCCAGCTGT TGGGCAGCGC TCATGAAGTC CAGAGGTTCC ACAGAGATGC TGATGAAACA
    AAAGAATGGA TAGAAGAGAA GAATCAAGCA TTAAATACCG ACAACTATGG ACATGACTTG
    GCCAGTGTGC AGGCTCTGCA GCGCAAGCAT GAAGGCTTTG AGAGAGACTT GGCAGCTCTT
    GGAGACAAGG TGAATTCTCT TGGTGAAACT GCCCAGCGTC TGATCCAGTC ACATCCAGAA
    TCAGCTGAAG ATCTTCAAGA AAAATGTACT GAGTTGAACC AAGCCTGGAG TAGTCTGGGA
    AAACGTGCTG ACCAACGCAA AGAGAAGCTT GGAGATTCTC ATGACCTGCA GCGTTTCCTC
    AGTGATTTTA GAGACCTTAT GTCTTGGATC AATGGAATCC GGGGTCTGGT CTCCTCAGAT
    GAACTTGCAA AAGATGTGAC TGGAGCTGAA GCTCTGTTGG AAAGGCATCA GGAGCACCGT
    ACTGAAATAG ATGCACGGGC TGGCACTTTT CAGGCATTTG AACAGTTTGG ACAGCAGCTT
    CTGGCTCGTG GACACTATGC CAGCCCAGAG ATTAAGGAGA AACTGGATAT TCTAGATCAA
    GAACGGACAG ACCTAGAGAA GGCCTGGGTC CAGCGCAGAA TGATGCTAGA CCAGTGCTTA
    GAACTACAGC TGTTTCATCG GGATTGTGAA CAAGCTGAAA ACTGGATGGC TGCCCGAGAG
    GCGTTCCTAA ATACAGAAGA CAAAGGAGAC TCCTTAGACA GCGTGGAGGC ACTCATCAAG
    AAGCATGAAG ATTTCGATAA AGCAATCAAT GTCCAGGAAG AGAAAATTGC TGTCTTGCAG
    TCTTTTGCTG ACCAACTGAT TGCTGCTGAT CATTATGCAA AAGGAGTCAT TGCTAACAGA
    CGCAATGAGG TTCTGGACAG GTGGCGTCGT CTGAAGGCTC AGATGATTGA GAAGAGATCT
    AAGCTGGGAG AATCTCAGAC CCTCCAACAG TTCAGTCGTG ATGTTGATGA AATAGAAGCT
    TGGATCAGTG AAAAGCTTCA AACTGCAAGT GATGAGTCAT ATAAGGATCC CACAAACATC
    CAGCTTTCCA AACTGCTGAG CAAGCACCAG AAGCACCAAG CCTTTGAAGC TGAGCTCCAC
    GCCAACGCAG ATCGGATTCG TGGAGTCATT GAAATGGGGA ACTCTCTTAT TGAAAGAGGA
    GCGTGTGCTG GCAGCGAGGA TGCTGTGAAG GCTCGGCTGG CTGCCCTGGC TGACCAATGG
    GAGTTCCTGG TCCAGAAGTC ATCAGAGAAG AGTCAGAAAC TGAAAGAAGC AAATAAACAG
    CAGAATTTCA ATACCGGCAT CAAGGACTTT GATTTCTGGC TTTCAGAGGT GGAAGCTTTG
    TTGGCATCTG AAGACTATGG GAAGGACTTG GCATCAGTGA ACAACCTTCT GAAGAAGCAC
    CAATTACTGG AAGCTGATAT ATCTGCTCAT GAGGATCGGC TGAAGGACCT GAACAGCCAG
    GCTGACAGTT TGATGACCAG CAGTGCTTTC GATACCTCCC AAGTAAAGGA TAAACGTGAA
    ACTATAAATG GGCGCTTCCA GAGAATCAAG AGCATGGCAG CTGCCCGCCG TGCGAAGCTC
    AACGAGTCGC ACCGCTTGCA TCAGTTCTTC CGTGACATGG ATGATGAGGA GTCCTGGATC
    AAAGAGAAGA AACTGTTGGT GAGCTCAGAG GACTATGGCA GAGACCTGAC TGGTGTGCAG
    AACCTGAGGA AGAAACATAA GCGCTTGGAA GCAGAATTAG CTGCCCATGA ACCTGCTATC
    CAGGGTGTTC TAGACACTGG CAAGAAGCTT TCAGATGATA ACACAATTGG GAAGGAGGAG
    ATACAGCAAA GACTGGCTCA GTTTGTGGAC CACTGGAAAG AGTTAAAACA GCTGGCAGCT
    GCTCGGGGGC AGCGTCTGGA GGAGTCCCTG GAGTACCAAC AGTTTGTAGC AAATGTTGAG
    GAAGAGGAGG CATGGATCAA CGAGAAAATG ACATTGGTAG CCAGTGAGGA TTATGGAGAC
    ACACTTGCTG CTATCCAGGG CTTGCTGAAG AAGCATGAAG CATTCGAGAC TGACTTTACT
    GTCCACAAAG ACAGAGTGAA TGATGTTTGC GCTAATGGAG AGGATCTCAT TAAAAAGAAT
    AATCACCATG TGGAGAACAT TACTGCTAAG ATGAAGGGCC TCAAGGGCAA GGTATCAGAT
    CTGGAGAAAG CCGCAGCTCA GAGGAAAGCC AAACTGGATG AGAACTCTGC CTTCCTCCAG
    TTCAACTGGA AAGCAGATGT GGTGGAGTCA TGGATAGGTG AGAAGGAAAA CAGTCTGAAG
    ACAGATGATT ATGGCCGTGA CCTCTCCTCT GTGCAAACAC TACTCACCAA ACAGGAAACC
    TTTGATGCTG GACTTCAGGC TTTCCAGCAG GAGGGAATTG CAAACATCAC TGCTCTGAAA
    GACCAGCTAC TGGCAGCCAA ACATATCCAA TCAAAGGCCA TTGAGGTTCG TCACGCTTCC
    TTGATGAAAC GCTGGAATCA GCTGCTTGCT AATTCTGCAG CCAGGAAAAA GAAACTCTTG
    GAGGCTCAGG AGCACTTCAG AAAGGTTGAA GATCTCTTCT TGACTTTTGC CAAGAAGGCC
    TCTGCCTTCA ACAGTTGGTT TGAGAATGCT GAGGAGGACC TGACGGATCC CGTGCGCTGC
    AATTCCCTGG AAGAAATCAA AGCACTGCGA GAAGCCCACG ATGCGTTCCG TTCCTCACTT
    AGCTCTGCCC AAGCTGACTT CAACCAGCTG GCAGAGCTTG ATCGGCAGAT CAAGAGCTTC
    CGTGTAGCCT CCAACCCGTA CACTTGGTTT ACTATGGAGG CTCTTGAAGA AACTTGGAGG
    AATCTGCAGA AAATTATCAA GGAGCGTGAA TTGGAGTTGC AGAAGGAGCA GCGGAGGCAG
    GAAGAAAATG ACAAGTTGCG CCAGGAGTTT GCTCAGCATG CTAATGCCTT CCACCAGTGG
    ATTCAGGAGA CAAGGACTTA CCTGCTAGAT GGGTCCTGCA TGGTGGAGGA GTCGGGAACA
    CTGGAATCCC AGCTGGAAGC TACTAAACGC AAGCACCAGG AGATCCGGGC TATGAGGAGC
    CAGCTGAAGA AGATTGAGGA CCTTGGAGCA GCCATGGAAG AGGCACTTAT CTTGGACAAC
    AAATACACAG AGCACAGCAC CGTGGGGCTG GCGCAGCAGT GGGACCAGCT TGACCAGCTG
    GGAATGAGAA TGCAGCACAA TCTGGAACAG CAGATTCAAG CTCGAAACAC AACTGGAGTC
    ACAGAGGAGG CCCTGAAGGA GTTCAGCATG ATGTTCAAGC ACTTTGACAA GGACAAATCT
    GGACGTCTTA ATCACCAGGA GTTTAAGTCT TGCTTGCGCT CCCTTGGCTA TGACCTGCCC
    ATGGTTGAGG AAGGAGAGCC TGACCCCGAA TTTGAGTCTA TTCTTGACAC TGTTGATCCC
    AACAGGGATG GCCACGTCTC GCTGCAGGAG TACATGGCGT TCATGATCAG CAGGGAAACA
    GAGAACGTGA AATCCAGCGA GGAGATTGAG AGTGCTTTCC GTGCCCTCAG CTCGGAGGGG
    AAGCCTTACG TGACCAAGGA GGAGCTCTAC CAGAACCTGA CCCGGGAGCA GGCCGACTAC
    TGCATTTCTC ACATGAAGCC CTACATGGAT GGCAAGGGCA GAGAACTTCC TTCTGCCTAC
    GACTACATAG AATTTACACG TTCACTCTTT GTGAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa43312
    Clone ID Related Accession (Same CDS sequence) XM_015279286.1 , XM_015279286.2
    Accession Version XM_015279286.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7539bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015279286.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
    TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG
    TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC
    TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT
    GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT
    GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG
    ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAT CTTTCACGCC TTACAGGACT
    GCACAGCGCC CAATAGTAAC AGCCACCCAA GTTGGGTCCC ACATACGAAC TCGTCTACAG
    GAGCTGCACC GACTATGGGA GTTACTGCTG GAAAAAATGA GAGAGAAGGG AGTGAAGCTG
    TTGCAAGCAC AGAAGCTGGT GCAATTCTTA CGGGAATGTG AAGATGTCAT GGACTGGATC
    AATGACAAGG AAGCAATAGT GACCTCAGAA GAGCTTGGAC AGGACTTAGA GCATGTTGAG
    GTTTTGCAGA AGAAATTTGA AGAGTTCCAA ACAGATCTGG CAGCTCATGA GGAGAGAGTG
    AATGAAGTGA ACCAGTTCGC TGGCAAACTT ATCCAGGAAC AGCACCCTGA GGAGGAACTT
    ATAAAGTCCA AACAGGATGA AGTAAATGCA AGCTGGCAGC GTCTTAAGGG GCTTGCCCAA
    CAGAGGCAAG GAAAACTCTT TGGGGCAGCT GAAGTTCAGC GCTTCAACAG GGATGTGGAT
    GAAACTATCA GCTGGATTAA GGAGAAAGGG CAGTTGATGG CCTCAGATGA CTTTGGCAGA
    GACTTGGCCA GCGTGCAAGC TTTACTACGT AAGCATGAAG GCCTGGAAAG AGATCTTGCA
    GCTTTGGAGG ATAAGGTTAA GGCCCTCTGT GCAGAAGCTG ACCGTTTGCA GCAGTCTCAC
    CCTATAAATG CTTCTCAAAT TCAAGTGAAA CGGGAGGAAC TGATTGCCAA CTGGGAGCAG
    ATCCGAACTC TGGCAGCAGA GAGGCATGCT CGCCTTAATG ACTCCTACAG GTTGCAGCGC
    TTTCTTGCAG ATTTCCGGGA CCTCACTAGC TGGGTAACTG AGATGAAGGC TCTGATAAAT
    GCTGATGAGC TTGCCAATGA TGTGGCTGGA GCAGAAGCCC TTCTAGATAG ACATCAGGAA
    CATAAGGGAG AAATTGATGC TCATGAGGAT AGCTTCAGAT CTGCTGATGA GTCTGGGCAG
    GCTTTGCTTG CAGCTGGGCA CTATGCTTCT GATGAAGTTA AAGAAAAGCT GACTATCCTC
    TCAGATGAAA GATCAGCCTT GCTGGAACTA TGGGAGCTTC GCAGGCAACA GTATGAGCAG
    TGCATGGATC TGCAGCTTTT CTACAGAGAT ACTGAACAAG TTGACAACTG GATGAGCAAA
    CAAGAAGCTT TTCTGCTGAA CGAAGACCTT GGTGATTCTC TGGATAGCGT GGAGGCTCTT
    CTAAAGAAGC ATGAAGATTT TGAGAAATCC CTAAGTGCTC AAGAGGAGAA GATCACAGCA
    CTGGATGAGT TTGCTACTAA ACTGATTCAA AATAACCATT ATGCCATGGA TGACGTTGCT
    ACGCGCAGAG ATGCTCTTCT GAGTCGCCGA AATGCTCTTC ATGAAAGAGC CATGAAGCGC
    CGTGCCCAAC TGGCAGACTC TTTCCATCTC CAGCAGTTTT TCAGGGACTC TGATGAACTG
    AAGAGTTGGG TTAATGAAAA AATGAAAACT GCTACCGATG AGGCTTACAA GGATCCATCC
    AACTTGCAAG GTAAAGTTCA GAAGCATCAG GCTTTTGAAG CAGAGCTTTC TGCTAATCAG
    AGTCGTATTG ATGCACTGGA GAAAGCTGGC CAGAAGTTGA TTGATGTCAA TCACTATGCA
    TCTGATGAGG TGGCAGCTCG CATGAATGAA GTCATCAGCT TGTGGAAGAA ACTTCTGGAA
    GCCACCGAGC TCAAAGGTAT AAAGCTGCGT GAAGCCAATC AACAGCAGCA ATTTAATCGC
    AATGTAGAAG ACATTGAGTT GTGGCTGTAT GAAGTGGAGG GTCACTTGGC TTCTGATGAT
    TATGGAAAAG ATCTTACCAG CGTTCAGAAT CTTCAGAAGA AACATGCCCT GCTAGAGGCA
    GATGTTGCTG CCCATCAGGA TCGGATAGAT GGCATTACCA TCCAGGCACG CCAGTTCCAA
    GATGCTGGGC ACTTTGATGC TGACAATATC AAGAAGAAAC AAGAAGCTTT AGTAGCTCGT
    TATGAAGCTC TGAAGGATCC TATGGTGGCT CGCAAGCAGA AACTTGCAGA TTCTCTTCGC
    CTGCAGCAGC TTTTCCGTGA CATCGAGGAT GAAGAGACCT GGATCAGGGA AAAGGAACCT
    ATTGCAGCCT CAACAAACCG AGGCAAGGAC TTAATTGGTG TCCAGAATCT GCTAAAGAAG
    CACCAGGCTT TGCAGGCAGA AATTGCAGGC CATGAGCCTC GCATTAAAGC AGTCACACAA
    AAAGGAAATG CGATGGTGGA AGAGGGACAT TTTGCTGCTG AAGATGTGAA GATCAAATTG
    AATGAACTAA ACCAAAAATG GGACTCTCTG AAAGCCAAGG CATCTCAACG GCGACAGGAT
    CTAGAGGATT CTCTGCAAGC TCAGCAGTAT TTTGCTGATG CTAATGAGGC AGAATCATGG
    ATGAGGGAAA AGGAGCCCAT TGTAGGCAGC ACGGATTATG GAAAGGATGA AGACTCTGCT
    GAGGCTCTCC TGAAGAAGCA TGAAGCTTTG ATGTCTGATC TTTCTGCTTA CGGCAGTAGC
    ATACAGGCAT TAAGGGAACA GGCCCAGTCC TGCAGGCAAC AAGTTGCTCC CACTGATGAT
    GAAACTGGAA AAGAGCTTGT GCTAGCACTC TATGATTACC AAGAGAAGAG TCCTCGGGAG
    GTGACTATGA AGAAAGGAGA TATTCTAACC CTGCTCAACA GCACCAACAA GGACTGGTGG
    AAGGTTGAAG TTAACGATCG TCAGGGCTTT GTACCAGCTG CCTATGTGAA AAAACTAGAT
    CCTGCCCAGT CTGCATCCCG AGAGAATCTT CTAGAAGAGC AAGGCAGCAT AGCATTGCGA
    CAGGAGCAAA TTGACAACCA GACTCTCATT ACTAAGGAAG TCGGCAGTGT ATCTCTGCGT
    ATGAAACAGG TCGAAGAACT GTATCATTCC CTTCTTGAGC TGGGAGAAAA ACGTAAAGGC
    ATGCTAGAAA AAAGCTGCAA GAAGTTTATG CTTTTCCGTG AGGCTAATGA GCTTCAACAG
    TGGATCAATG AGAAGGAAGC TGCGCTCACT AATGAGGAAG TGGGTGCTGA TTTGGAGCAG
    GTGGAGGTGC TACAGAAGAA GTTTGATGAT TTTCAGAAGG ATTTAAAAGC TAATGAGTCA
    CGACTGAAGG ACATAAACAA GGTTGCAAAT GACCTGGAGT CTGAAGGGCT GATGGCAGAG
    GAAGTGCAAG CAGTAGAGCA CCAGGAAGTC TATGGAATGA TGCCCAGAGA TGAAACTGAT
    TCTAAGACAG CCTCTCCTTG GAAGTCTGCA CGTATGATGG TACACACAGT GGCAACGTTT
    AACTCAATCA AGGAACTGAA TGAACGCTGG AGATCCCTGC AGCAGTTGGC AGAGGAGCGA
    AGCCAGCTGT TGGGCAGCGC TCATGAAGTC CAGAGGTTCC ACAGAGATGC TGATGAAACA
    AAAGAATGGA TAGAAGAGAA GAATCAAGCA TTAAATACCG ACAACTATGG ACATGACTTG
    GCCAGTGTGC AGGCTCTGCA GCGCAAGCAT GAAGGCTTTG AGAGAGACTT GGCAGCTCTT
    GGAGACAAGG TGAATTCTCT TGGTGAAACT GCCCAGCGTC TGATCCAGTC ACATCCAGAA
    TCAGCTGAAG ATCTTCAAGA AAAATGTACT GAGTTGAACC AAGCCTGGAG TAGTCTGGGA
    AAACGTGCTG ACCAACGCAA AGAGAAGCTT GGAGATTCTC ATGACCTGCA GCGTTTCCTC
    AGTGATTTTA GAGACCTTAT GTCTTGGATC AATGGAATCC GGGGTCTGGT CTCCTCAGAT
    GAACTTGCAA AAGATGTGAC TGGAGCTGAA GCTCTGTTGG AAAGGCATCA GGAGCACCGT
    ACTGAAATAG ATGCACGGGC TGGCACTTTT CAGGCATTTG AACAGTTTGG ACAGCAGCTT
    CTGGCTCGTG GACACTATGC CAGCCCAGAG ATTAAGGAGA AACTGGATAT TCTAGATCAA
    GAACGGACAG ACCTAGAGAA GGCCTGGGTC CAGCGCAGAA TGATGCTAGA CCAGTGCTTA
    GAACTACAGC TGTTTCATCG GGATTGTGAA CAAGCTGAAA ACTGGATGGC TGCCCGAGAG
    GCGTTCCTAA ATACAGAAGA CAAAGGAGAC TCCTTAGACA GCGTGGAGGC ACTCATCAAG
    AAGCATGAAG ATTTCGATAA AGCAATCAAT GTCCAGGAAG AGAAAATTGC TGTCTTGCAG
    TCTTTTGCTG ACCAACTGAT TGCTGCTGAT CATTATGCAA AAGGAGTCAT TGCTAACAGA
    CGCAATGAGG TTCTGGACAG GTGGCGTCGT CTGAAGGCTC AGATGATTGA GAAGAGATCT
    AAGCTGGGAG AATCTCAGAC CCTCCAACAG TTCAGTCGTG ATGTTGATGA AATAGAAGCT
    TGGATCAGTG AAAAGCTTCA AACTGCAAGT GATGAGTCAT ATAAGGATCC CACAAACATC
    CAGCTTTCCA AACTGCTGAG CAAGCACCAG AAGCACCAAG CCTTTGAAGC TGAGCTCCAC
    GCCAACGCAG ATCGGATTCG TGGAGTCATT GAAATGGGGA ACTCTCTTAT TGAAAGAGGA
    GCGTGTGCTG GCAGCGAGGA TGCTGTGAAG GCTCGGCTGG CTGCCCTGGC TGACCAATGG
    GAGTTCCTGG TCCAGAAGTC ATCAGAGAAG AGTCAGAAAC TGAAAGAAGC AAATAAACAG
    CAGAATTTCA ATACCGGCAT CAAGGACTTT GATTTCTGGC TTTCAGAGGT GGAAGCTTTG
    TTGGCATCTG AAGACTATGG GAAGGACTTG GCATCAGTGA ACAACCTTCT GAAGAAGCAC
    CAATTACTGG AAGCTGATAT ATCTGCTCAT GAGGATCGGC TGAAGGACCT GAACAGCCAG
    GCTGACAGTT TGATGACCAG CAGTGCTTTC GATACCTCCC AAGTAAAGGA TAAACGTGAA
    ACTATAAATG GGCGCTTCCA GAGAATCAAG AGCATGGCAG CTGCCCGCCG TGCGAAGCTC
    AACGAGTCGC ACCGCTTGCA TCAGTTCTTC CGTGACATGG ATGATGAGGA GTCCTGGATC
    AAAGAGAAGA AACTGTTGGT GAGCTCAGAG GACTATGGCA GAGACCTGAC TGGTGTGCAG
    AACCTGAGGA AGAAACATAA GCGCTTGGAA GCAGAATTAG CTGCCCATGA ACCTGCTATC
    CAGGGTGTTC TAGACACTGG CAAGAAGCTT TCAGATGATA ACACAATTGG GAAGGAGGAG
    ATACAGCAAA GACTGGCTCA GTTTGTGGAC CACTGGAAAG AGTTAAAACA GCTGGCAGCT
    GCTCGGGGGC AGCGTCTGGA GGAGTCCCTG GAGTACCAAC AGTTTGTAGC AAATGTTGAG
    GAAGAGGAGG CATGGATCAA CGAGAAAATG ACATTGGTAG CCAGTGAGGA TTATGGAGAC
    ACACTTGCTG CTATCCAGGG CTTGCTGAAG AAGCATGAAG CATTCGAGAC TGACTTTACT
    GTCCACAAAG ACAGAGTGAA TGATGTTTGC GCTAATGGAG AGGATCTCAT TAAAAAGAAT
    AATCACCATG TGGAGAACAT TACTGCTAAG ATGAAGGGCC TCAAGGGCAA GGTATCAGAT
    CTGGAGAAAG CCGCAGCTCA GAGGAAAGCC AAACTGGATG AGAACTCTGC CTTCCTCCAG
    TTCAACTGGA AAGCAGATGT GGTGGAGTCA TGGATAGGTG AGAAGGAAAA CAGTCTGAAG
    ACAGATGATT ATGGCCGTGA CCTCTCCTCT GTGCAAACAC TACTCACCAA ACAGGAAACC
    TTTGATGCTG GACTTCAGGC TTTCCAGCAG GAGGGAATTG CAAACATCAC TGCTCTGAAA
    GACCAGCTAC TGGCAGCCAA ACATATCCAA TCAAAGGCCA TTGAGGTTCG TCACGCTTCC
    TTGATGAAAC GCTGGAATCA GCTGCTTGCT AATTCTGCAG CCAGGAAAAA GAAACTCTTG
    GAGGCTCAGG AGCACTTCAG AAAGGTTGAA GATCTCTTCT TGACTTTTGC CAAGAAGGCC
    TCTGCCTTCA ACAGTTGGTT TGAGAATGCT GAGGAGGACC TGACGGATCC CGTGCGCTGC
    AATTCCCTGG AAGAAATCAA AGCACTGCGA GAAGCCCACG ATGCGTTCCG TTCCTCACTT
    AGCTCTGCCC AAGCTGACTT CAACCAGCTG GCAGAGCTTG ATCGGCAGAT CAAGAGCTTC
    CGTGTAGCCT CCAACCCGTA CACTTGGTTT ACTATGGAGG CTCTTGAAGA AACTTGGAGG
    AATCTGCAGA AAATTATCAA GGAGCGTGAA TTGGAGTTGC AGAAGGAGCA GCGGAGGCAG
    GAAGAAAATG ACAAGTTGCG CCAGGAGTTT GCTCAGCATG CTAATGCCTT CCACCAGTGG
    ATTCAGGAGA CAAGGACTTA CCTGCTAGAT GGGTCCTGCA TGGTGGAGGA GTCGGGAACA
    CTGGAATCCC AGCTGGAAGC TACTAAACGC AAGCACCAGG AGATCCGGGC TATGAGGAGC
    CAGCTGAAGA AGATTGAGGA CCTTGGAGCA GCCATGGAAG AGGCACTTAT CTTGGACAAC
    AAATACACAG AGCACAGCAC CGTGGGGCTG GCGCAGCAGT GGGACCAGCT TGACCAGCTG
    GGAATGAGAA TGCAGCACAA TCTGGAACAG CAGATTCAAG CTCGAAACAC AACTGGAGTC
    ACAGAGGAGG CCCTGAAGGA GTTCAGCATG ATGTTCAAGC ACTTTGACAA GGACAAATCT
    GGACGTCTTA ATCACCAGGA GTTTAAGTCT TGCTTGCGCT CCCTTGGCTA TGACCTGCCC
    ATGGTTGAGG AAGGAGAGCC TGACCCCGAA TTTGAGTCTA TTCTTGACAC TGTTGATCCC
    AACAGGGATG GCCACGTCTC GCTGCAGGAG TACATGGCGT TCATGATCAG CAGGGAAACA
    GAGAACGTGA AATCCAGCGA GGAGATTGAG AGTGCTTTCC GTGCCCTCAG CTCGGAGGGG
    AAGCCTTACG TGACCAAGGA GGAGCTCTAC CAGAACCTGA CCCGGGAGCA GGCCGACTAC
    TGCATTTCTC ACATGAAGCC CTACATGGAT GGCAAGGGCA GAGAACTTCC TTCTGCCTAC
    GACTACATAG AATTTACACG TTCACTCTTT GTGAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015134772.1
    CDS13..7551
    Translation

    Target ORF information:

    RefSeq Version XM_015279286.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015279286.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
    TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG
    TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC
    TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT
    GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT
    GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG
    ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAT CTTTCACGCC TTACAGGACT
    GCACAGCGCC CAATAGTAAC AGCCACCCAA GTTGGGTCCC ACATACGAAC TCGTCTACAG
    GAGCTGCACC GACTATGGGA GTTACTGCTG GAAAAAATGA GAGAGAAGGG AGTGAAGCTG
    TTGCAAGCAC AGAAGCTGGT GCAATTCTTA CGGGAATGTG AAGATGTCAT GGACTGGATC
    AATGACAAGG AAGCAATAGT GACCTCAGAA GAGCTTGGAC AGGACTTAGA GCATGTTGAG
    GTTTTGCAGA AGAAATTTGA AGAGTTCCAA ACAGATCTGG CAGCTCATGA GGAGAGAGTG
    AATGAAGTGA ACCAGTTCGC TGGCAAACTT ATCCAGGAAC AGCACCCTGA GGAGGAACTT
    ATAAAGTCCA AACAGGATGA AGTAAATGCA AGCTGGCAGC GTCTTAAGGG GCTTGCCCAA
    CAGAGGCAAG GAAAACTCTT TGGGGCAGCT GAAGTTCAGC GCTTCAACAG GGATGTGGAT
    GAAACTATCA GCTGGATTAA GGAGAAAGGG CAGTTGATGG CCTCAGATGA CTTTGGCAGA
    GACTTGGCCA GCGTGCAAGC TTTACTACGT AAGCATGAAG GCCTGGAAAG AGATCTTGCA
    GCTTTGGAGG ATAAGGTTAA GGCCCTCTGT GCAGAAGCTG ACCGTTTGCA GCAGTCTCAC
    CCTATAAATG CTTCTCAAAT TCAAGTGAAA CGGGAGGAAC TGATTGCCAA CTGGGAGCAG
    ATCCGAACTC TGGCAGCAGA GAGGCATGCT CGCCTTAATG ACTCCTACAG GTTGCAGCGC
    TTTCTTGCAG ATTTCCGGGA CCTCACTAGC TGGGTAACTG AGATGAAGGC TCTGATAAAT
    GCTGATGAGC TTGCCAATGA TGTGGCTGGA GCAGAAGCCC TTCTAGATAG ACATCAGGAA
    CATAAGGGAG AAATTGATGC TCATGAGGAT AGCTTCAGAT CTGCTGATGA GTCTGGGCAG
    GCTTTGCTTG CAGCTGGGCA CTATGCTTCT GATGAAGTTA AAGAAAAGCT GACTATCCTC
    TCAGATGAAA GATCAGCCTT GCTGGAACTA TGGGAGCTTC GCAGGCAACA GTATGAGCAG
    TGCATGGATC TGCAGCTTTT CTACAGAGAT ACTGAACAAG TTGACAACTG GATGAGCAAA
    CAAGAAGCTT TTCTGCTGAA CGAAGACCTT GGTGATTCTC TGGATAGCGT GGAGGCTCTT
    CTAAAGAAGC ATGAAGATTT TGAGAAATCC CTAAGTGCTC AAGAGGAGAA GATCACAGCA
    CTGGATGAGT TTGCTACTAA ACTGATTCAA AATAACCATT ATGCCATGGA TGACGTTGCT
    ACGCGCAGAG ATGCTCTTCT GAGTCGCCGA AATGCTCTTC ATGAAAGAGC CATGAAGCGC
    CGTGCCCAAC TGGCAGACTC TTTCCATCTC CAGCAGTTTT TCAGGGACTC TGATGAACTG
    AAGAGTTGGG TTAATGAAAA AATGAAAACT GCTACCGATG AGGCTTACAA GGATCCATCC
    AACTTGCAAG GTAAAGTTCA GAAGCATCAG GCTTTTGAAG CAGAGCTTTC TGCTAATCAG
    AGTCGTATTG ATGCACTGGA GAAAGCTGGC CAGAAGTTGA TTGATGTCAA TCACTATGCA
    TCTGATGAGG TGGCAGCTCG CATGAATGAA GTCATCAGCT TGTGGAAGAA ACTTCTGGAA
    GCCACCGAGC TCAAAGGTAT AAAGCTGCGT GAAGCCAATC AACAGCAGCA ATTTAATCGC
    AATGTAGAAG ACATTGAGTT GTGGCTGTAT GAAGTGGAGG GTCACTTGGC TTCTGATGAT
    TATGGAAAAG ATCTTACCAG CGTTCAGAAT CTTCAGAAGA AACATGCCCT GCTAGAGGCA
    GATGTTGCTG CCCATCAGGA TCGGATAGAT GGCATTACCA TCCAGGCACG CCAGTTCCAA
    GATGCTGGGC ACTTTGATGC TGACAATATC AAGAAGAAAC AAGAAGCTTT AGTAGCTCGT
    TATGAAGCTC TGAAGGATCC TATGGTGGCT CGCAAGCAGA AACTTGCAGA TTCTCTTCGC
    CTGCAGCAGC TTTTCCGTGA CATCGAGGAT GAAGAGACCT GGATCAGGGA AAAGGAACCT
    ATTGCAGCCT CAACAAACCG AGGCAAGGAC TTAATTGGTG TCCAGAATCT GCTAAAGAAG
    CACCAGGCTT TGCAGGCAGA AATTGCAGGC CATGAGCCTC GCATTAAAGC AGTCACACAA
    AAAGGAAATG CGATGGTGGA AGAGGGACAT TTTGCTGCTG AAGATGTGAA GATCAAATTG
    AATGAACTAA ACCAAAAATG GGACTCTCTG AAAGCCAAGG CATCTCAACG GCGACAGGAT
    CTAGAGGATT CTCTGCAAGC TCAGCAGTAT TTTGCTGATG CTAATGAGGC AGAATCATGG
    ATGAGGGAAA AGGAGCCCAT TGTAGGCAGC ACGGATTATG GAAAGGATGA AGACTCTGCT
    GAGGCTCTCC TGAAGAAGCA TGAAGCTTTG ATGTCTGATC TTTCTGCTTA CGGCAGTAGC
    ATACAGGCAT TAAGGGAACA GGCCCAGTCC TGCAGGCAAC AAGTTGCTCC CACTGATGAT
    GAAACTGGAA AAGAGCTTGT GCTAGCACTC TATGATTACC AAGAGAAGAG TCCTCGGGAG
    GTGACTATGA AGAAAGGAGA TATTCTAACC CTGCTCAACA GCACCAACAA GGACTGGTGG
    AAGGTTGAAG TTAACGATCG TCAGGGCTTT GTACCAGCTG CCTATGTGAA AAAACTAGAT
    CCTGCCCAGT CTGCATCCCG AGAGAATCTT CTAGAAGAGC AAGGCAGCAT AGCATTGCGA
    CAGGAGCAAA TTGACAACCA GACTCTCATT ACTAAGGAAG TCGGCAGTGT ATCTCTGCGT
    ATGAAACAGG TCGAAGAACT GTATCATTCC CTTCTTGAGC TGGGAGAAAA ACGTAAAGGC
    ATGCTAGAAA AAAGCTGCAA GAAGTTTATG CTTTTCCGTG AGGCTAATGA GCTTCAACAG
    TGGATCAATG AGAAGGAAGC TGCGCTCACT AATGAGGAAG TGGGTGCTGA TTTGGAGCAG
    GTGGAGGTGC TACAGAAGAA GTTTGATGAT TTTCAGAAGG ATTTAAAAGC TAATGAGTCA
    CGACTGAAGG ACATAAACAA GGTTGCAAAT GACCTGGAGT CTGAAGGGCT GATGGCAGAG
    GAAGTGCAAG CAGTAGAGCA CCAGGAAGTC TATGGAATGA TGCCCAGAGA TGAAACTGAT
    TCTAAGACAG CCTCTCCTTG GAAGTCTGCA CGTATGATGG TACACACAGT GGCAACGTTT
    AACTCAATCA AGGAACTGAA TGAACGCTGG AGATCCCTGC AGCAGTTGGC AGAGGAGCGA
    AGCCAGCTGT TGGGCAGCGC TCATGAAGTC CAGAGGTTCC ACAGAGATGC TGATGAAACA
    AAAGAATGGA TAGAAGAGAA GAATCAAGCA TTAAATACCG ACAACTATGG ACATGACTTG
    GCCAGTGTGC AGGCTCTGCA GCGCAAGCAT GAAGGCTTTG AGAGAGACTT GGCAGCTCTT
    GGAGACAAGG TGAATTCTCT TGGTGAAACT GCCCAGCGTC TGATCCAGTC ACATCCAGAA
    TCAGCTGAAG ATCTTCAAGA AAAATGTACT GAGTTGAACC AAGCCTGGAG TAGTCTGGGA
    AAACGTGCTG ACCAACGCAA AGAGAAGCTT GGAGATTCTC ATGACCTGCA GCGTTTCCTC
    AGTGATTTTA GAGACCTTAT GTCTTGGATC AATGGAATCC GGGGTCTGGT CTCCTCAGAT
    GAACTTGCAA AAGATGTGAC TGGAGCTGAA GCTCTGTTGG AAAGGCATCA GGAGCACCGT
    ACTGAAATAG ATGCACGGGC TGGCACTTTT CAGGCATTTG AACAGTTTGG ACAGCAGCTT
    CTGGCTCGTG GACACTATGC CAGCCCAGAG ATTAAGGAGA AACTGGATAT TCTAGATCAA
    GAACGGACAG ACCTAGAGAA GGCCTGGGTC CAGCGCAGAA TGATGCTAGA CCAGTGCTTA
    GAACTACAGC TGTTTCATCG GGATTGTGAA CAAGCTGAAA ACTGGATGGC TGCCCGAGAG
    GCGTTCCTAA ATACAGAAGA CAAAGGAGAC TCCTTAGACA GCGTGGAGGC ACTCATCAAG
    AAGCATGAAG ATTTCGATAA AGCAATCAAT GTCCAGGAAG AGAAAATTGC TGTCTTGCAG
    TCTTTTGCTG ACCAACTGAT TGCTGCTGAT CATTATGCAA AAGGAGTCAT TGCTAACAGA
    CGCAATGAGG TTCTGGACAG GTGGCGTCGT CTGAAGGCTC AGATGATTGA GAAGAGATCT
    AAGCTGGGAG AATCTCAGAC CCTCCAACAG TTCAGTCGTG ATGTTGATGA AATAGAAGCT
    TGGATCAGTG AAAAGCTTCA AACTGCAAGT GATGAGTCAT ATAAGGATCC CACAAACATC
    CAGCTTTCCA AACTGCTGAG CAAGCACCAG AAGCACCAAG CCTTTGAAGC TGAGCTCCAC
    GCCAACGCAG ATCGGATTCG TGGAGTCATT GAAATGGGGA ACTCTCTTAT TGAAAGAGGA
    GCGTGTGCTG GCAGCGAGGA TGCTGTGAAG GCTCGGCTGG CTGCCCTGGC TGACCAATGG
    GAGTTCCTGG TCCAGAAGTC ATCAGAGAAG AGTCAGAAAC TGAAAGAAGC AAATAAACAG
    CAGAATTTCA ATACCGGCAT CAAGGACTTT GATTTCTGGC TTTCAGAGGT GGAAGCTTTG
    TTGGCATCTG AAGACTATGG GAAGGACTTG GCATCAGTGA ACAACCTTCT GAAGAAGCAC
    CAATTACTGG AAGCTGATAT ATCTGCTCAT GAGGATCGGC TGAAGGACCT GAACAGCCAG
    GCTGACAGTT TGATGACCAG CAGTGCTTTC GATACCTCCC AAGTAAAGGA TAAACGTGAA
    ACTATAAATG GGCGCTTCCA GAGAATCAAG AGCATGGCAG CTGCCCGCCG TGCGAAGCTC
    AACGAGTCGC ACCGCTTGCA TCAGTTCTTC CGTGACATGG ATGATGAGGA GTCCTGGATC
    AAAGAGAAGA AACTGTTGGT GAGCTCAGAG GACTATGGCA GAGACCTGAC TGGTGTGCAG
    AACCTGAGGA AGAAACATAA GCGCTTGGAA GCAGAATTAG CTGCCCATGA ACCTGCTATC
    CAGGGTGTTC TAGACACTGG CAAGAAGCTT TCAGATGATA ACACAATTGG GAAGGAGGAG
    ATACAGCAAA GACTGGCTCA GTTTGTGGAC CACTGGAAAG AGTTAAAACA GCTGGCAGCT
    GCTCGGGGGC AGCGTCTGGA GGAGTCCCTG GAGTACCAAC AGTTTGTAGC AAATGTTGAG
    GAAGAGGAGG CATGGATCAA CGAGAAAATG ACATTGGTAG CCAGTGAGGA TTATGGAGAC
    ACACTTGCTG CTATCCAGGG CTTGCTGAAG AAGCATGAAG CATTCGAGAC TGACTTTACT
    GTCCACAAAG ACAGAGTGAA TGATGTTTGC GCTAATGGAG AGGATCTCAT TAAAAAGAAT
    AATCACCATG TGGAGAACAT TACTGCTAAG ATGAAGGGCC TCAAGGGCAA GGTATCAGAT
    CTGGAGAAAG CCGCAGCTCA GAGGAAAGCC AAACTGGATG AGAACTCTGC CTTCCTCCAG
    TTCAACTGGA AAGCAGATGT GGTGGAGTCA TGGATAGGTG AGAAGGAAAA CAGTCTGAAG
    ACAGATGATT ATGGCCGTGA CCTCTCCTCT GTGCAAACAC TACTCACCAA ACAGGAAACC
    TTTGATGCTG GACTTCAGGC TTTCCAGCAG GAGGGAATTG CAAACATCAC TGCTCTGAAA
    GACCAGCTAC TGGCAGCCAA ACATATCCAA TCAAAGGCCA TTGAGGTTCG TCACGCTTCC
    TTGATGAAAC GCTGGAATCA GCTGCTTGCT AATTCTGCAG CCAGGAAAAA GAAACTCTTG
    GAGGCTCAGG AGCACTTCAG AAAGGTTGAA GATCTCTTCT TGACTTTTGC CAAGAAGGCC
    TCTGCCTTCA ACAGTTGGTT TGAGAATGCT GAGGAGGACC TGACGGATCC CGTGCGCTGC
    AATTCCCTGG AAGAAATCAA AGCACTGCGA GAAGCCCACG ATGCGTTCCG TTCCTCACTT
    AGCTCTGCCC AAGCTGACTT CAACCAGCTG GCAGAGCTTG ATCGGCAGAT CAAGAGCTTC
    CGTGTAGCCT CCAACCCGTA CACTTGGTTT ACTATGGAGG CTCTTGAAGA AACTTGGAGG
    AATCTGCAGA AAATTATCAA GGAGCGTGAA TTGGAGTTGC AGAAGGAGCA GCGGAGGCAG
    GAAGAAAATG ACAAGTTGCG CCAGGAGTTT GCTCAGCATG CTAATGCCTT CCACCAGTGG
    ATTCAGGAGA CAAGGACTTA CCTGCTAGAT GGGTCCTGCA TGGTGGAGGA GTCGGGAACA
    CTGGAATCCC AGCTGGAAGC TACTAAACGC AAGCACCAGG AGATCCGGGC TATGAGGAGC
    CAGCTGAAGA AGATTGAGGA CCTTGGAGCA GCCATGGAAG AGGCACTTAT CTTGGACAAC
    AAATACACAG AGCACAGCAC CGTGGGGCTG GCGCAGCAGT GGGACCAGCT TGACCAGCTG
    GGAATGAGAA TGCAGCACAA TCTGGAACAG CAGATTCAAG CTCGAAACAC AACTGGAGTC
    ACAGAGGAGG CCCTGAAGGA GTTCAGCATG ATGTTCAAGC ACTTTGACAA GGACAAATCT
    GGACGTCTTA ATCACCAGGA GTTTAAGTCT TGCTTGCGCT CCCTTGGCTA TGACCTGCCC
    ATGGTTGAGG AAGGAGAGCC TGACCCCGAA TTTGAGTCTA TTCTTGACAC TGTTGATCCC
    AACAGGGATG GCCACGTCTC GCTGCAGGAG TACATGGCGT TCATGATCAG CAGGGAAACA
    GAGAACGTGA AATCCAGCGA GGAGATTGAG AGTGCTTTCC GTGCCCTCAG CTCGGAGGGG
    AAGCCTTACG TGACCAAGGA GGAGCTCTAC CAGAACCTGA CCCGGGAGCA GGCCGACTAC
    TGCATTTCTC ACATGAAGCC CTACATGGAT GGCAAGGGCA GAGAACTTCC TTCTGCCTAC
    GACTACATAG AATTTACACG TTCACTCTTT GTGAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa43313
    Clone ID Related Accession (Same CDS sequence) XM_015279287.1 , XM_015279287.2
    Accession Version XM_015279287.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7533bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
    TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG
    TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC
    TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT
    GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT
    GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG
    ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAA CTCGTCTACA GGAGCTGCAC
    CGACTATGGG AGTTACTGCT GGAAAAAATG AGAGAGAAGG GAGTGAAGCT GTTGCAAGCA
    CAGAAGCTGG TGCAATTCTT ACGGGAATGT GAAGATGTCA TGGACTGGAT CAATGACAAG
    GAAGCAATAG TGACCTCAGA AGAGCTTGGA CAGGACTTAG AGCATGTTGA GGTTTTGCAG
    AAGAAATTTG AAGAGTTCCA AACAGATCTG GCAGCTCATG AGGAGAGAGT GAATGAAGTG
    AACCAGTTCG CTGGCAAACT TATCCAGGAA CAGCACCCTG AGGAGGAACT TATAAAGTCC
    AAACAGGATG AAGTAAATGC AAGCTGGCAG CGTCTTAAGG GGCTTGCCCA ACAGAGGCAA
    GGAAAACTCT TTGGGGCAGC TGAAGTTCAG CGCTTCAACA GGGATGTGGA TGAAACTATC
    AGCTGGATTA AGGAGAAAGG GCAGTTGATG GCCTCAGATG ACTTTGGCAG AGACTTGGCC
    AGCGTGCAAG CTTTACTACG TAAGCATGAA GGCCTGGAAA GAGATCTTGC AGCTTTGGAG
    GATAAGGTTA AGGCCCTCTG TGCAGAAGCT GACCGTTTGC AGCAGTCTCA CCCTATAAAT
    GCTTCTCAAA TTCAAGTGAA ACGGGAGGAA CTGATTGCCA ACTGGGAGCA GATCCGAACT
    CTGGCAGCAG AGAGGCATGC TCGCCTTAAT GACTCCTACA GGTTGCAGCG CTTTCTTGCA
    GATTTCCGGG ACCTCACTAG CTGGGTAACT GAGATGAAGG CTCTGATAAA TGCTGATGAG
    CTTGCCAATG ATGTGGCTGG AGCAGAAGCC CTTCTAGATA GACATCAGGA ACATAAGGGA
    GAAATTGATG CTCATGAGGA TAGCTTCAGA TCTGCTGATG AGTCTGGGCA GGCTTTGCTT
    GCAGCTGGGC ACTATGCTTC TGATGAAGTT AAAGAAAAGC TGACTATCCT CTCAGATGAA
    AGATCAGCCT TGCTGGAACT ATGGGAGCTT CGCAGGCAAC AGTATGAGCA GTGCATGGAT
    CTGCAGCTTT TCTACAGAGA TACTGAACAA GTTGACAACT GGATGAGCAA ACAAGAAGCT
    TTTCTGCTGA ACGAAGACCT TGGTGATTCT CTGGATAGCG TGGAGGCTCT TCTAAAGAAG
    CATGAAGATT TTGAGAAATC CCTAAGTGCT CAAGAGGAGA AGATCACAGC ACTGGATGAG
    TTTGCTACTA AACTGATTCA AAATAACCAT TATGCCATGG ATGACGTTGC TACGCGCAGA
    GATGCTCTTC TGAGTCGCCG AAATGCTCTT CATGAAAGAG CCATGAAGCG CCGTGCCCAA
    CTGGCAGACT CTTTCCATCT CCAGCAGTTT TTCAGGGACT CTGATGAACT GAAGAGTTGG
    GTTAATGAAA AAATGAAAAC TGCTACCGAT GAGGCTTACA AGGATCCATC CAACTTGCAA
    GGTAAAGTTC AGAAGCATCA GGCTTTTGAA GCAGAGCTTT CTGCTAATCA GAGTCGTATT
    GATGCACTGG AGAAAGCTGG CCAGAAGTTG ATTGATGTCA ATCACTATGC ATCTGATGAG
    GTGGCAGCTC GCATGAATGA AGTCATCAGC TTGTGGAAGA AACTTCTGGA AGCCACCGAG
    CTCAAAGGTA TAAAGCTGCG TGAAGCCAAT CAACAGCAGC AATTTAATCG CAATGTAGAA
    GACATTGAGT TGTGGCTGTA TGAAGTGGAG GGTCACTTGG CTTCTGATGA TTATGGAAAA
    GATCTTACCA GCGTTCAGAA TCTTCAGAAG AAACATGCCC TGCTAGAGGC AGATGTTGCT
    GCCCATCAGG ATCGGATAGA TGGCATTACC ATCCAGGCAC GCCAGTTCCA AGATGCTGGG
    CACTTTGATG CTGACAATAT CAAGAAGAAA CAAGAAGCTT TAGTAGCTCG TTATGAAGCT
    CTGAAGGATC CTATGGTGGC TCGCAAGCAG AAACTTGCAG ATTCTCTTCG CCTGCAGCAG
    CTTTTCCGTG ACATCGAGGA TGAAGAGACC TGGATCAGGG AAAAGGAACC TATTGCAGCC
    TCAACAAACC GAGGCAAGGA CTTAATTGGT GTCCAGAATC TGCTAAAGAA GCACCAGGCT
    TTGCAGGCAG AAATTGCAGG CCATGAGCCT CGCATTAAAG CAGTCACACA AAAAGGAAAT
    GCGATGGTGG AAGAGGGACA TTTTGCTGCT GAAGATGTGA AGATCAAATT GAATGAACTA
    AACCAAAAAT GGGACTCTCT GAAAGCCAAG GCATCTCAAC GGCGACAGGA TCTAGAGGAT
    TCTCTGCAAG CTCAGCAGTA TTTTGCTGAT GCTAATGAGG CAGAATCATG GATGAGGGAA
    AAGGAGCCCA TTGTAGGCAG CACGGATTAT GGAAAGGATG AAGACTCTGC TGAGGCTCTC
    CTGAAGAAGC ATGAAGCTTT GATGTCTGAT CTTTCTGCTT ACGGCAGTAG CATACAGGCA
    TTAAGGGAAC AGGCCCAGTC CTGCAGGCAA CAAGTTGCTC CCACTGATGA TGAAACTGGA
    AAAGAGCTTG TGCTAGCACT CTATGATTAC CAAGAGAAGA GTCCTCGGGA GGTGACTATG
    AAGAAAGGAG ATATTCTAAC CCTGCTCAAC AGCACCAACA AGGACTGGTG GAAGGTTGAA
    GTTAACGATC GTCAGGGCTT TGTACCAGCT GCCTATGTGA AAAAACTAGA TCCTGCCCAG
    TCTGCATCCC GAGAGAATCT TCTAGAAGAG CAAGGCAGCA TAGCATTGCG ACAGGAGCAA
    ATTGACAACC AGACTCTCAT TACTAAGGAA GTCGGCAGTG TATCTCTGCG TATGAAACAG
    GTCGAAGAAC TGTATCATTC CCTTCTTGAG CTGGGAGAAA AACGTAAAGG CATGCTAGAA
    AAAAGCTGCA AGAAGTTTAT GCTTTTCCGT GAGGCTAATG AGCTTCAACA GTGGATCAAT
    GAGAAGGAAG CTGCGCTCAC TAATGAGGAA GTGGGTGCTG ATTTGGAGCA GGTGGAGGTG
    CTACAGAAGA AGTTTGATGA TTTTCAGAAG GATTTAAAAG CTAATGAGTC ACGACTGAAG
    GACATAAACA AGGTTGCAAA TGACCTGGAG TCTGAAGGGC TGATGGCAGA GGAAGTGCAA
    GCAGTAGAGC ACCAGGAAGT CTATGGAATG ATGCCCAGAG ATGAAACTGA TTCTAAGACA
    GCCTCTCCTT GGAAGTCTGC ACGTATGATG GTACACACAG TGGCAACGTT TAACTCAATC
    AAGGAACTGA ATGAACGCTG GAGATCCCTG CAGCAGTTGG CAGAGGAGCG AAGCCAGCTG
    TTGGGCAGCG CTCATGAAGT CCAGAGGTTC CACAGAGATG CTGATGAAAC AAAAGAATGG
    ATAGAAGAGA AGAATCAAGC ATTAAATACC GACAACTATG GACATGACTT GGCCAGTGTG
    CAGGCTCTGC AGCGCAAGCA TGAAGGCTTT GAGAGAGACT TGGCAGCTCT TGGAGACAAG
    GTGAATTCTC TTGGTGAAAC TGCCCAGCGT CTGATCCAGT CACATCCAGA ATCAGCTGAA
    GATCTTCAAG AAAAATGTAC TGAGTTGAAC CAAGCCTGGA GTAGTCTGGG AAAACGTGCT
    GACCAACGCA AAGAGAAGCT TGGAGATTCT CATGACCTGC AGCGTTTCCT CAGTGATTTT
    AGAGACCTTA TGTCTTGGAT CAATGGAATC CGGGGTCTGG TCTCCTCAGA TGAACTTGCA
    AAAGATGTGA CTGGAGCTGA AGCTCTGTTG GAAAGGCATC AGGAGCACCG TACTGAAATA
    GATGCACGGG CTGGCACTTT TCAGGCATTT GAACAGTTTG GACAGCAGCT TCTGGCTCGT
    GGACACTATG CCAGCCCAGA GATTAAGGAG AAACTGGATA TTCTAGATCA AGAACGGACA
    GACCTAGAGA AGGCCTGGGT CCAGCGCAGA ATGATGCTAG ACCAGTGCTT AGAACTACAG
    CTGTTTCATC GGGATTGTGA ACAAGCTGAA AACTGGATGG CTGCCCGAGA GGCGTTCCTA
    AATACAGAAG ACAAAGGAGA CTCCTTAGAC AGCGTGGAGG CACTCATCAA GAAGCATGAA
    GATTTCGATA AAGCAATCAA TGTCCAGGAA GAGAAAATTG CTGTCTTGCA GTCTTTTGCT
    GACCAACTGA TTGCTGCTGA TCATTATGCA AAAGGAGTCA TTGCTAACAG ACGCAATGAG
    GTTCTGGACA GGTGGCGTCG TCTGAAGGCT CAGATGATTG AGAAGAGATC TAAGCTGGGA
    GAATCTCAGA CCCTCCAACA GTTCAGTCGT GATGTTGATG AAATAGAAGC TTGGATCAGT
    GAAAAGCTTC AAACTGCAAG TGATGAGTCA TATAAGGATC CCACAAACAT CCAGCTTTCC
    AAACTGCTGA GCAAGCACCA GAAGCACCAA GCCTTTGAAG CTGAGCTCCA CGCCAACGCA
    GATCGGATTC GTGGAGTCAT TGAAATGGGG AACTCTCTTA TTGAAAGAGG AGCGTGTGCT
    GGCAGCGAGG ATGCTGTGAA GGCTCGGCTG GCTGCCCTGG CTGACCAATG GGAGTTCCTG
    GTCCAGAAGT CATCAGAGAA GAGTCAGAAA CTGAAAGAAG CAAATAAACA GCAGAATTTC
    AATACCGGCA TCAAGGACTT TGATTTCTGG CTTTCAGAGG TGGAAGCTTT GTTGGCATCT
    GAAGACTATG GGAAGGACTT GGCATCAGTG AACAACCTTC TGAAGAAGCA CCAATTACTG
    GAAGCTGATA TATCTGCTCA TGAGGATCGG CTGAAGGACC TGAACAGCCA GGCTGACAGT
    TTGATGACCA GCAGTGCTTT CGATACCTCC CAAGTAAAGG ATAAACGTGA AACTATAAAT
    GGGCGCTTCC AGAGAATCAA GAGCATGGCA GCTGCCCGCC GTGCGAAGCT CAACGAGTCG
    CACCGCTTGC ATCAGTTCTT CCGTGACATG GATGATGAGG AGTCCTGGAT CAAAGAGAAG
    AAACTGTTGG TGAGCTCAGA GGACTATGGC AGAGACCTGA CTGGTGTGCA GAACCTGAGG
    AAGAAACATA AGCGCTTGGA AGCAGAATTA GCTGCCCATG AACCTGCTAT CCAGGGTGTT
    CTAGACACTG GCAAGAAGCT TTCAGATGAT AACACAATTG GGAAGGAGGA GATACAGCAA
    AGACTGGCTC AGTTTGTGGA CCACTGGAAA GAGTTAAAAC AGCTGGCAGC TGCTCGGGGG
    CAGCGTCTGG AGGAGTCCCT GGAGTACCAA CAGTTTGTAG CAAATGTTGA GGAAGAGGAG
    GCATGGATCA ACGAGAAAAT GACATTGGTA GCCAGTGAGG ATTATGGAGA CACACTTGCT
    GCTATCCAGG GCTTGCTGAA GAAGCATGAA GCATTCGAGA CTGACTTTAC TGTCCACAAA
    GACAGAGTGA ATGATGTTTG CGCTAATGGA GAGGATCTCA TTAAAAAGAA TAATCACCAT
    GTGGAGAACA TTACTGCTAA GATGAAGGGC CTCAAGGGCA AGGTATCAGA TCTGGAGAAA
    GCCGCAGCTC AGAGGAAAGC CAAACTGGAT GAGAACTCTG CCTTCCTCCA GTTCAACTGG
    AAAGCAGATG TGGTGGAGTC ATGGATAGGT GAGAAGGAAA ACAGTCTGAA GACAGATGAT
    TATGGCCGTG ACCTCTCCTC TGTGCAAACA CTACTCACCA AACAGGAAAC CTTTGATGCT
    GGACTTCAGG CTTTCCAGCA GGAGGGAATT GCAAACATCA CTGCTCTGAA AGACCAGCTA
    CTGGCAGCCA AACATATCCA ATCAAAGGCC ATTGAGGTTC GTCACGCTTC CTTGATGAAA
    CGCTGGAATC AGCTGCTTGC TAATTCTGCA GCCAGGAAAA AGAAACTCTT GGAGGCTCAG
    GAGCACTTCA GAAAGGTTGA AGATCTCTTC TTGACTTTTG CCAAGAAGGC CTCTGCCTTC
    AACAGTTGGT TTGAGAATGC TGAGGAGGAC CTGACGGATC CCGTGCGCTG CAATTCCCTG
    GAAGAAATCA AAGCACTGCG AGAAGCCCAC GATGCGTTCC GTTCCTCACT TAGCTCTGCC
    CAAGCTGACT TCAACCAGCT GGCAGAGCTT GATCGGCAGA TCAAGAGCTT CCGTGTAGCC
    TCCAACCCGT ACACTTGGTT TACTATGGAG GCTCTTGAAG AAACTTGGAG GAATCTGCAG
    AAAATTATCA AGGAGCGTGA ATTGGAGTTG CAGAAGGAGC AGCGGAGGCA GGAAGAAAAT
    GACAAGTTGC GCCAGGAGTT TGCTCAGCAT GCTAATGCCT TCCACCAGTG GATTCAGGAG
    ACAAGGACTT ACCTGCTAGA TGGCATAGCC TATCGTCGTG TCATTCGTGT CTATCAGTAT
    GAAGTTGGGG ATGATCTATC TGGAAGGTCC TGCATGGTGG AGGAGTCGGG AACACTGGAA
    TCCCAGCTGG AAGCTACTAA ACGCAAGCAC CAGGAGATCC GGGCTATGAG GAGCCAGCTG
    AAGAAGATTG AGGACCTTGG AGCAGCCATG GAAGAGGCAC TTATCTTGGA CAACAAATAC
    ACAGAGCACA GCACCGTGGG GCTGGCGCAG CAGTGGGACC AGCTTGACCA GCTGGGAATG
    AGAATGCAGC ACAATCTGGA ACAGCAGATT CAAGCTCGAA ACACAACTGG AGTCACAGAG
    GAGGCCCTGA AGGAGTTCAG CATGATGTTC AAGCACTTTG ACAAGGACAA ATCTGGACGT
    CTTAATCACC AGGAGTTTAA GTCTTGCTTG CGCTCCCTTG GCTATGACCT GCCCATGGTT
    GAGGAAGGAG AGCCTGACCC CGAATTTGAG TCTATTCTTG ACACTGTTGA TCCCAACAGG
    GATGGCCACG TCTCGCTGCA GGAGTACATG GCGTTCATGA TCAGCAGGGA AACAGAGAAC
    GTGAAATCCA GCGAGGAGAT TGAGAGTGCT TTCCGTGCCC TCAGCTCGGA GGGGAAGCCT
    TACGTGACCA AGGAGGAGCT CTACCAGAAC CTGACCCGGG AGCAGGCCGA CTACTGCATT
    TCTCACATGA AGCCCTACAT GGATGGCAAG GGCAGAGAAC TTCCTTCTGC CTACGACTAC
    ATAGAATTTA CACGTTCACT CTTTGTGAAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015134773.1
    CDS16..7548
    Translation

    Target ORF information:

    RefSeq Version XM_015279287.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015279287.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
    TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG
    TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC
    TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT
    GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT
    GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG
    ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAA CTCGTCTACA GGAGCTGCAC
    CGACTATGGG AGTTACTGCT GGAAAAAATG AGAGAGAAGG GAGTGAAGCT GTTGCAAGCA
    CAGAAGCTGG TGCAATTCTT ACGGGAATGT GAAGATGTCA TGGACTGGAT CAATGACAAG
    GAAGCAATAG TGACCTCAGA AGAGCTTGGA CAGGACTTAG AGCATGTTGA GGTTTTGCAG
    AAGAAATTTG AAGAGTTCCA AACAGATCTG GCAGCTCATG AGGAGAGAGT GAATGAAGTG
    AACCAGTTCG CTGGCAAACT TATCCAGGAA CAGCACCCTG AGGAGGAACT TATAAAGTCC
    AAACAGGATG AAGTAAATGC AAGCTGGCAG CGTCTTAAGG GGCTTGCCCA ACAGAGGCAA
    GGAAAACTCT TTGGGGCAGC TGAAGTTCAG CGCTTCAACA GGGATGTGGA TGAAACTATC
    AGCTGGATTA AGGAGAAAGG GCAGTTGATG GCCTCAGATG ACTTTGGCAG AGACTTGGCC
    AGCGTGCAAG CTTTACTACG TAAGCATGAA GGCCTGGAAA GAGATCTTGC AGCTTTGGAG
    GATAAGGTTA AGGCCCTCTG TGCAGAAGCT GACCGTTTGC AGCAGTCTCA CCCTATAAAT
    GCTTCTCAAA TTCAAGTGAA ACGGGAGGAA CTGATTGCCA ACTGGGAGCA GATCCGAACT
    CTGGCAGCAG AGAGGCATGC TCGCCTTAAT GACTCCTACA GGTTGCAGCG CTTTCTTGCA
    GATTTCCGGG ACCTCACTAG CTGGGTAACT GAGATGAAGG CTCTGATAAA TGCTGATGAG
    CTTGCCAATG ATGTGGCTGG AGCAGAAGCC CTTCTAGATA GACATCAGGA ACATAAGGGA
    GAAATTGATG CTCATGAGGA TAGCTTCAGA TCTGCTGATG AGTCTGGGCA GGCTTTGCTT
    GCAGCTGGGC ACTATGCTTC TGATGAAGTT AAAGAAAAGC TGACTATCCT CTCAGATGAA
    AGATCAGCCT TGCTGGAACT ATGGGAGCTT CGCAGGCAAC AGTATGAGCA GTGCATGGAT
    CTGCAGCTTT TCTACAGAGA TACTGAACAA GTTGACAACT GGATGAGCAA ACAAGAAGCT
    TTTCTGCTGA ACGAAGACCT TGGTGATTCT CTGGATAGCG TGGAGGCTCT TCTAAAGAAG
    CATGAAGATT TTGAGAAATC CCTAAGTGCT CAAGAGGAGA AGATCACAGC ACTGGATGAG
    TTTGCTACTA AACTGATTCA AAATAACCAT TATGCCATGG ATGACGTTGC TACGCGCAGA
    GATGCTCTTC TGAGTCGCCG AAATGCTCTT CATGAAAGAG CCATGAAGCG CCGTGCCCAA
    CTGGCAGACT CTTTCCATCT CCAGCAGTTT TTCAGGGACT CTGATGAACT GAAGAGTTGG
    GTTAATGAAA AAATGAAAAC TGCTACCGAT GAGGCTTACA AGGATCCATC CAACTTGCAA
    GGTAAAGTTC AGAAGCATCA GGCTTTTGAA GCAGAGCTTT CTGCTAATCA GAGTCGTATT
    GATGCACTGG AGAAAGCTGG CCAGAAGTTG ATTGATGTCA ATCACTATGC ATCTGATGAG
    GTGGCAGCTC GCATGAATGA AGTCATCAGC TTGTGGAAGA AACTTCTGGA AGCCACCGAG
    CTCAAAGGTA TAAAGCTGCG TGAAGCCAAT CAACAGCAGC AATTTAATCG CAATGTAGAA
    GACATTGAGT TGTGGCTGTA TGAAGTGGAG GGTCACTTGG CTTCTGATGA TTATGGAAAA
    GATCTTACCA GCGTTCAGAA TCTTCAGAAG AAACATGCCC TGCTAGAGGC AGATGTTGCT
    GCCCATCAGG ATCGGATAGA TGGCATTACC ATCCAGGCAC GCCAGTTCCA AGATGCTGGG
    CACTTTGATG CTGACAATAT CAAGAAGAAA CAAGAAGCTT TAGTAGCTCG TTATGAAGCT
    CTGAAGGATC CTATGGTGGC TCGCAAGCAG AAACTTGCAG ATTCTCTTCG CCTGCAGCAG
    CTTTTCCGTG ACATCGAGGA TGAAGAGACC TGGATCAGGG AAAAGGAACC TATTGCAGCC
    TCAACAAACC GAGGCAAGGA CTTAATTGGT GTCCAGAATC TGCTAAAGAA GCACCAGGCT
    TTGCAGGCAG AAATTGCAGG CCATGAGCCT CGCATTAAAG CAGTCACACA AAAAGGAAAT
    GCGATGGTGG AAGAGGGACA TTTTGCTGCT GAAGATGTGA AGATCAAATT GAATGAACTA
    AACCAAAAAT GGGACTCTCT GAAAGCCAAG GCATCTCAAC GGCGACAGGA TCTAGAGGAT
    TCTCTGCAAG CTCAGCAGTA TTTTGCTGAT GCTAATGAGG CAGAATCATG GATGAGGGAA
    AAGGAGCCCA TTGTAGGCAG CACGGATTAT GGAAAGGATG AAGACTCTGC TGAGGCTCTC
    CTGAAGAAGC ATGAAGCTTT GATGTCTGAT CTTTCTGCTT ACGGCAGTAG CATACAGGCA
    TTAAGGGAAC AGGCCCAGTC CTGCAGGCAA CAAGTTGCTC CCACTGATGA TGAAACTGGA
    AAAGAGCTTG TGCTAGCACT CTATGATTAC CAAGAGAAGA GTCCTCGGGA GGTGACTATG
    AAGAAAGGAG ATATTCTAAC CCTGCTCAAC AGCACCAACA AGGACTGGTG GAAGGTTGAA
    GTTAACGATC GTCAGGGCTT TGTACCAGCT GCCTATGTGA AAAAACTAGA TCCTGCCCAG
    TCTGCATCCC GAGAGAATCT TCTAGAAGAG CAAGGCAGCA TAGCATTGCG ACAGGAGCAA
    ATTGACAACC AGACTCTCAT TACTAAGGAA GTCGGCAGTG TATCTCTGCG TATGAAACAG
    GTCGAAGAAC TGTATCATTC CCTTCTTGAG CTGGGAGAAA AACGTAAAGG CATGCTAGAA
    AAAAGCTGCA AGAAGTTTAT GCTTTTCCGT GAGGCTAATG AGCTTCAACA GTGGATCAAT
    GAGAAGGAAG CTGCGCTCAC TAATGAGGAA GTGGGTGCTG ATTTGGAGCA GGTGGAGGTG
    CTACAGAAGA AGTTTGATGA TTTTCAGAAG GATTTAAAAG CTAATGAGTC ACGACTGAAG
    GACATAAACA AGGTTGCAAA TGACCTGGAG TCTGAAGGGC TGATGGCAGA GGAAGTGCAA
    GCAGTAGAGC ACCAGGAAGT CTATGGAATG ATGCCCAGAG ATGAAACTGA TTCTAAGACA
    GCCTCTCCTT GGAAGTCTGC ACGTATGATG GTACACACAG TGGCAACGTT TAACTCAATC
    AAGGAACTGA ATGAACGCTG GAGATCCCTG CAGCAGTTGG CAGAGGAGCG AAGCCAGCTG
    TTGGGCAGCG CTCATGAAGT CCAGAGGTTC CACAGAGATG CTGATGAAAC AAAAGAATGG
    ATAGAAGAGA AGAATCAAGC ATTAAATACC GACAACTATG GACATGACTT GGCCAGTGTG
    CAGGCTCTGC AGCGCAAGCA TGAAGGCTTT GAGAGAGACT TGGCAGCTCT TGGAGACAAG
    GTGAATTCTC TTGGTGAAAC TGCCCAGCGT CTGATCCAGT CACATCCAGA ATCAGCTGAA
    GATCTTCAAG AAAAATGTAC TGAGTTGAAC CAAGCCTGGA GTAGTCTGGG AAAACGTGCT
    GACCAACGCA AAGAGAAGCT TGGAGATTCT CATGACCTGC AGCGTTTCCT CAGTGATTTT
    AGAGACCTTA TGTCTTGGAT CAATGGAATC CGGGGTCTGG TCTCCTCAGA TGAACTTGCA
    AAAGATGTGA CTGGAGCTGA AGCTCTGTTG GAAAGGCATC AGGAGCACCG TACTGAAATA
    GATGCACGGG CTGGCACTTT TCAGGCATTT GAACAGTTTG GACAGCAGCT TCTGGCTCGT
    GGACACTATG CCAGCCCAGA GATTAAGGAG AAACTGGATA TTCTAGATCA AGAACGGACA
    GACCTAGAGA AGGCCTGGGT CCAGCGCAGA ATGATGCTAG ACCAGTGCTT AGAACTACAG
    CTGTTTCATC GGGATTGTGA ACAAGCTGAA AACTGGATGG CTGCCCGAGA GGCGTTCCTA
    AATACAGAAG ACAAAGGAGA CTCCTTAGAC AGCGTGGAGG CACTCATCAA GAAGCATGAA
    GATTTCGATA AAGCAATCAA TGTCCAGGAA GAGAAAATTG CTGTCTTGCA GTCTTTTGCT
    GACCAACTGA TTGCTGCTGA TCATTATGCA AAAGGAGTCA TTGCTAACAG ACGCAATGAG
    GTTCTGGACA GGTGGCGTCG TCTGAAGGCT CAGATGATTG AGAAGAGATC TAAGCTGGGA
    GAATCTCAGA CCCTCCAACA GTTCAGTCGT GATGTTGATG AAATAGAAGC TTGGATCAGT
    GAAAAGCTTC AAACTGCAAG TGATGAGTCA TATAAGGATC CCACAAACAT CCAGCTTTCC
    AAACTGCTGA GCAAGCACCA GAAGCACCAA GCCTTTGAAG CTGAGCTCCA CGCCAACGCA
    GATCGGATTC GTGGAGTCAT TGAAATGGGG AACTCTCTTA TTGAAAGAGG AGCGTGTGCT
    GGCAGCGAGG ATGCTGTGAA GGCTCGGCTG GCTGCCCTGG CTGACCAATG GGAGTTCCTG
    GTCCAGAAGT CATCAGAGAA GAGTCAGAAA CTGAAAGAAG CAAATAAACA GCAGAATTTC
    AATACCGGCA TCAAGGACTT TGATTTCTGG CTTTCAGAGG TGGAAGCTTT GTTGGCATCT
    GAAGACTATG GGAAGGACTT GGCATCAGTG AACAACCTTC TGAAGAAGCA CCAATTACTG
    GAAGCTGATA TATCTGCTCA TGAGGATCGG CTGAAGGACC TGAACAGCCA GGCTGACAGT
    TTGATGACCA GCAGTGCTTT CGATACCTCC CAAGTAAAGG ATAAACGTGA AACTATAAAT
    GGGCGCTTCC AGAGAATCAA GAGCATGGCA GCTGCCCGCC GTGCGAAGCT CAACGAGTCG
    CACCGCTTGC ATCAGTTCTT CCGTGACATG GATGATGAGG AGTCCTGGAT CAAAGAGAAG
    AAACTGTTGG TGAGCTCAGA GGACTATGGC AGAGACCTGA CTGGTGTGCA GAACCTGAGG
    AAGAAACATA AGCGCTTGGA AGCAGAATTA GCTGCCCATG AACCTGCTAT CCAGGGTGTT
    CTAGACACTG GCAAGAAGCT TTCAGATGAT AACACAATTG GGAAGGAGGA GATACAGCAA
    AGACTGGCTC AGTTTGTGGA CCACTGGAAA GAGTTAAAAC AGCTGGCAGC TGCTCGGGGG
    CAGCGTCTGG AGGAGTCCCT GGAGTACCAA CAGTTTGTAG CAAATGTTGA GGAAGAGGAG
    GCATGGATCA ACGAGAAAAT GACATTGGTA GCCAGTGAGG ATTATGGAGA CACACTTGCT
    GCTATCCAGG GCTTGCTGAA GAAGCATGAA GCATTCGAGA CTGACTTTAC TGTCCACAAA
    GACAGAGTGA ATGATGTTTG CGCTAATGGA GAGGATCTCA TTAAAAAGAA TAATCACCAT
    GTGGAGAACA TTACTGCTAA GATGAAGGGC CTCAAGGGCA AGGTATCAGA TCTGGAGAAA
    GCCGCAGCTC AGAGGAAAGC CAAACTGGAT GAGAACTCTG CCTTCCTCCA GTTCAACTGG
    AAAGCAGATG TGGTGGAGTC ATGGATAGGT GAGAAGGAAA ACAGTCTGAA GACAGATGAT
    TATGGCCGTG ACCTCTCCTC TGTGCAAACA CTACTCACCA AACAGGAAAC CTTTGATGCT
    GGACTTCAGG CTTTCCAGCA GGAGGGAATT GCAAACATCA CTGCTCTGAA AGACCAGCTA
    CTGGCAGCCA AACATATCCA ATCAAAGGCC ATTGAGGTTC GTCACGCTTC CTTGATGAAA
    CGCTGGAATC AGCTGCTTGC TAATTCTGCA GCCAGGAAAA AGAAACTCTT GGAGGCTCAG
    GAGCACTTCA GAAAGGTTGA AGATCTCTTC TTGACTTTTG CCAAGAAGGC CTCTGCCTTC
    AACAGTTGGT TTGAGAATGC TGAGGAGGAC CTGACGGATC CCGTGCGCTG CAATTCCCTG
    GAAGAAATCA AAGCACTGCG AGAAGCCCAC GATGCGTTCC GTTCCTCACT TAGCTCTGCC
    CAAGCTGACT TCAACCAGCT GGCAGAGCTT GATCGGCAGA TCAAGAGCTT CCGTGTAGCC
    TCCAACCCGT ACACTTGGTT TACTATGGAG GCTCTTGAAG AAACTTGGAG GAATCTGCAG
    AAAATTATCA AGGAGCGTGA ATTGGAGTTG CAGAAGGAGC AGCGGAGGCA GGAAGAAAAT
    GACAAGTTGC GCCAGGAGTT TGCTCAGCAT GCTAATGCCT TCCACCAGTG GATTCAGGAG
    ACAAGGACTT ACCTGCTAGA TGGCATAGCC TATCGTCGTG TCATTCGTGT CTATCAGTAT
    GAAGTTGGGG ATGATCTATC TGGAAGGTCC TGCATGGTGG AGGAGTCGGG AACACTGGAA
    TCCCAGCTGG AAGCTACTAA ACGCAAGCAC CAGGAGATCC GGGCTATGAG GAGCCAGCTG
    AAGAAGATTG AGGACCTTGG AGCAGCCATG GAAGAGGCAC TTATCTTGGA CAACAAATAC
    ACAGAGCACA GCACCGTGGG GCTGGCGCAG CAGTGGGACC AGCTTGACCA GCTGGGAATG
    AGAATGCAGC ACAATCTGGA ACAGCAGATT CAAGCTCGAA ACACAACTGG AGTCACAGAG
    GAGGCCCTGA AGGAGTTCAG CATGATGTTC AAGCACTTTG ACAAGGACAA ATCTGGACGT
    CTTAATCACC AGGAGTTTAA GTCTTGCTTG CGCTCCCTTG GCTATGACCT GCCCATGGTT
    GAGGAAGGAG AGCCTGACCC CGAATTTGAG TCTATTCTTG ACACTGTTGA TCCCAACAGG
    GATGGCCACG TCTCGCTGCA GGAGTACATG GCGTTCATGA TCAGCAGGGA AACAGAGAAC
    GTGAAATCCA GCGAGGAGAT TGAGAGTGCT TTCCGTGCCC TCAGCTCGGA GGGGAAGCCT
    TACGTGACCA AGGAGGAGCT CTACCAGAAC CTGACCCGGG AGCAGGCCGA CTACTGCATT
    TCTCACATGA AGCCCTACAT GGATGGCAAG GGCAGAGAAC TTCCTTCTGC CTACGACTAC
    ATAGAATTTA CACGTTCACT CTTTGTGAAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa43313
    Clone ID Related Accession (Same CDS sequence) XM_015279287.1 , XM_015279287.2
    Accession Version XM_015279287.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7533bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015279287.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
    TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG
    TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC
    TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT
    GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT
    GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG
    ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAA CTCGTCTACA GGAGCTGCAC
    CGACTATGGG AGTTACTGCT GGAAAAAATG AGAGAGAAGG GAGTGAAGCT GTTGCAAGCA
    CAGAAGCTGG TGCAATTCTT ACGGGAATGT GAAGATGTCA TGGACTGGAT CAATGACAAG
    GAAGCAATAG TGACCTCAGA AGAGCTTGGA CAGGACTTAG AGCATGTTGA GGTTTTGCAG
    AAGAAATTTG AAGAGTTCCA AACAGATCTG GCAGCTCATG AGGAGAGAGT GAATGAAGTG
    AACCAGTTCG CTGGCAAACT TATCCAGGAA CAGCACCCTG AGGAGGAACT TATAAAGTCC
    AAACAGGATG AAGTAAATGC AAGCTGGCAG CGTCTTAAGG GGCTTGCCCA ACAGAGGCAA
    GGAAAACTCT TTGGGGCAGC TGAAGTTCAG CGCTTCAACA GGGATGTGGA TGAAACTATC
    AGCTGGATTA AGGAGAAAGG GCAGTTGATG GCCTCAGATG ACTTTGGCAG AGACTTGGCC
    AGCGTGCAAG CTTTACTACG TAAGCATGAA GGCCTGGAAA GAGATCTTGC AGCTTTGGAG
    GATAAGGTTA AGGCCCTCTG TGCAGAAGCT GACCGTTTGC AGCAGTCTCA CCCTATAAAT
    GCTTCTCAAA TTCAAGTGAA ACGGGAGGAA CTGATTGCCA ACTGGGAGCA GATCCGAACT
    CTGGCAGCAG AGAGGCATGC TCGCCTTAAT GACTCCTACA GGTTGCAGCG CTTTCTTGCA
    GATTTCCGGG ACCTCACTAG CTGGGTAACT GAGATGAAGG CTCTGATAAA TGCTGATGAG
    CTTGCCAATG ATGTGGCTGG AGCAGAAGCC CTTCTAGATA GACATCAGGA ACATAAGGGA
    GAAATTGATG CTCATGAGGA TAGCTTCAGA TCTGCTGATG AGTCTGGGCA GGCTTTGCTT
    GCAGCTGGGC ACTATGCTTC TGATGAAGTT AAAGAAAAGC TGACTATCCT CTCAGATGAA
    AGATCAGCCT TGCTGGAACT ATGGGAGCTT CGCAGGCAAC AGTATGAGCA GTGCATGGAT
    CTGCAGCTTT TCTACAGAGA TACTGAACAA GTTGACAACT GGATGAGCAA ACAAGAAGCT
    TTTCTGCTGA ACGAAGACCT TGGTGATTCT CTGGATAGCG TGGAGGCTCT TCTAAAGAAG
    CATGAAGATT TTGAGAAATC CCTAAGTGCT CAAGAGGAGA AGATCACAGC ACTGGATGAG
    TTTGCTACTA AACTGATTCA AAATAACCAT TATGCCATGG ATGACGTTGC TACGCGCAGA
    GATGCTCTTC TGAGTCGCCG AAATGCTCTT CATGAAAGAG CCATGAAGCG CCGTGCCCAA
    CTGGCAGACT CTTTCCATCT CCAGCAGTTT TTCAGGGACT CTGATGAACT GAAGAGTTGG
    GTTAATGAAA AAATGAAAAC TGCTACCGAT GAGGCTTACA AGGATCCATC CAACTTGCAA
    GGTAAAGTTC AGAAGCATCA GGCTTTTGAA GCAGAGCTTT CTGCTAATCA GAGTCGTATT
    GATGCACTGG AGAAAGCTGG CCAGAAGTTG ATTGATGTCA ATCACTATGC ATCTGATGAG
    GTGGCAGCTC GCATGAATGA AGTCATCAGC TTGTGGAAGA AACTTCTGGA AGCCACCGAG
    CTCAAAGGTA TAAAGCTGCG TGAAGCCAAT CAACAGCAGC AATTTAATCG CAATGTAGAA
    GACATTGAGT TGTGGCTGTA TGAAGTGGAG GGTCACTTGG CTTCTGATGA TTATGGAAAA
    GATCTTACCA GCGTTCAGAA TCTTCAGAAG AAACATGCCC TGCTAGAGGC AGATGTTGCT
    GCCCATCAGG ATCGGATAGA TGGCATTACC ATCCAGGCAC GCCAGTTCCA AGATGCTGGG
    CACTTTGATG CTGACAATAT CAAGAAGAAA CAAGAAGCTT TAGTAGCTCG TTATGAAGCT
    CTGAAGGATC CTATGGTGGC TCGCAAGCAG AAACTTGCAG ATTCTCTTCG CCTGCAGCAG
    CTTTTCCGTG ACATCGAGGA TGAAGAGACC TGGATCAGGG AAAAGGAACC TATTGCAGCC
    TCAACAAACC GAGGCAAGGA CTTAATTGGT GTCCAGAATC TGCTAAAGAA GCACCAGGCT
    TTGCAGGCAG AAATTGCAGG CCATGAGCCT CGCATTAAAG CAGTCACACA AAAAGGAAAT
    GCGATGGTGG AAGAGGGACA TTTTGCTGCT GAAGATGTGA AGATCAAATT GAATGAACTA
    AACCAAAAAT GGGACTCTCT GAAAGCCAAG GCATCTCAAC GGCGACAGGA TCTAGAGGAT
    TCTCTGCAAG CTCAGCAGTA TTTTGCTGAT GCTAATGAGG CAGAATCATG GATGAGGGAA
    AAGGAGCCCA TTGTAGGCAG CACGGATTAT GGAAAGGATG AAGACTCTGC TGAGGCTCTC
    CTGAAGAAGC ATGAAGCTTT GATGTCTGAT CTTTCTGCTT ACGGCAGTAG CATACAGGCA
    TTAAGGGAAC AGGCCCAGTC CTGCAGGCAA CAAGTTGCTC CCACTGATGA TGAAACTGGA
    AAAGAGCTTG TGCTAGCACT CTATGATTAC CAAGAGAAGA GTCCTCGGGA GGTGACTATG
    AAGAAAGGAG ATATTCTAAC CCTGCTCAAC AGCACCAACA AGGACTGGTG GAAGGTTGAA
    GTTAACGATC GTCAGGGCTT TGTACCAGCT GCCTATGTGA AAAAACTAGA TCCTGCCCAG
    TCTGCATCCC GAGAGAATCT TCTAGAAGAG CAAGGCAGCA TAGCATTGCG ACAGGAGCAA
    ATTGACAACC AGACTCTCAT TACTAAGGAA GTCGGCAGTG TATCTCTGCG TATGAAACAG
    GTCGAAGAAC TGTATCATTC CCTTCTTGAG CTGGGAGAAA AACGTAAAGG CATGCTAGAA
    AAAAGCTGCA AGAAGTTTAT GCTTTTCCGT GAGGCTAATG AGCTTCAACA GTGGATCAAT
    GAGAAGGAAG CTGCGCTCAC TAATGAGGAA GTGGGTGCTG ATTTGGAGCA GGTGGAGGTG
    CTACAGAAGA AGTTTGATGA TTTTCAGAAG GATTTAAAAG CTAATGAGTC ACGACTGAAG
    GACATAAACA AGGTTGCAAA TGACCTGGAG TCTGAAGGGC TGATGGCAGA GGAAGTGCAA
    GCAGTAGAGC ACCAGGAAGT CTATGGAATG ATGCCCAGAG ATGAAACTGA TTCTAAGACA
    GCCTCTCCTT GGAAGTCTGC ACGTATGATG GTACACACAG TGGCAACGTT TAACTCAATC
    AAGGAACTGA ATGAACGCTG GAGATCCCTG CAGCAGTTGG CAGAGGAGCG AAGCCAGCTG
    TTGGGCAGCG CTCATGAAGT CCAGAGGTTC CACAGAGATG CTGATGAAAC AAAAGAATGG
    ATAGAAGAGA AGAATCAAGC ATTAAATACC GACAACTATG GACATGACTT GGCCAGTGTG
    CAGGCTCTGC AGCGCAAGCA TGAAGGCTTT GAGAGAGACT TGGCAGCTCT TGGAGACAAG
    GTGAATTCTC TTGGTGAAAC TGCCCAGCGT CTGATCCAGT CACATCCAGA ATCAGCTGAA
    GATCTTCAAG AAAAATGTAC TGAGTTGAAC CAAGCCTGGA GTAGTCTGGG AAAACGTGCT
    GACCAACGCA AAGAGAAGCT TGGAGATTCT CATGACCTGC AGCGTTTCCT CAGTGATTTT
    AGAGACCTTA TGTCTTGGAT CAATGGAATC CGGGGTCTGG TCTCCTCAGA TGAACTTGCA
    AAAGATGTGA CTGGAGCTGA AGCTCTGTTG GAAAGGCATC AGGAGCACCG TACTGAAATA
    GATGCACGGG CTGGCACTTT TCAGGCATTT GAACAGTTTG GACAGCAGCT TCTGGCTCGT
    GGACACTATG CCAGCCCAGA GATTAAGGAG AAACTGGATA TTCTAGATCA AGAACGGACA
    GACCTAGAGA AGGCCTGGGT CCAGCGCAGA ATGATGCTAG ACCAGTGCTT AGAACTACAG
    CTGTTTCATC GGGATTGTGA ACAAGCTGAA AACTGGATGG CTGCCCGAGA GGCGTTCCTA
    AATACAGAAG ACAAAGGAGA CTCCTTAGAC AGCGTGGAGG CACTCATCAA GAAGCATGAA
    GATTTCGATA AAGCAATCAA TGTCCAGGAA GAGAAAATTG CTGTCTTGCA GTCTTTTGCT
    GACCAACTGA TTGCTGCTGA TCATTATGCA AAAGGAGTCA TTGCTAACAG ACGCAATGAG
    GTTCTGGACA GGTGGCGTCG TCTGAAGGCT CAGATGATTG AGAAGAGATC TAAGCTGGGA
    GAATCTCAGA CCCTCCAACA GTTCAGTCGT GATGTTGATG AAATAGAAGC TTGGATCAGT
    GAAAAGCTTC AAACTGCAAG TGATGAGTCA TATAAGGATC CCACAAACAT CCAGCTTTCC
    AAACTGCTGA GCAAGCACCA GAAGCACCAA GCCTTTGAAG CTGAGCTCCA CGCCAACGCA
    GATCGGATTC GTGGAGTCAT TGAAATGGGG AACTCTCTTA TTGAAAGAGG AGCGTGTGCT
    GGCAGCGAGG ATGCTGTGAA GGCTCGGCTG GCTGCCCTGG CTGACCAATG GGAGTTCCTG
    GTCCAGAAGT CATCAGAGAA GAGTCAGAAA CTGAAAGAAG CAAATAAACA GCAGAATTTC
    AATACCGGCA TCAAGGACTT TGATTTCTGG CTTTCAGAGG TGGAAGCTTT GTTGGCATCT
    GAAGACTATG GGAAGGACTT GGCATCAGTG AACAACCTTC TGAAGAAGCA CCAATTACTG
    GAAGCTGATA TATCTGCTCA TGAGGATCGG CTGAAGGACC TGAACAGCCA GGCTGACAGT
    TTGATGACCA GCAGTGCTTT CGATACCTCC CAAGTAAAGG ATAAACGTGA AACTATAAAT
    GGGCGCTTCC AGAGAATCAA GAGCATGGCA GCTGCCCGCC GTGCGAAGCT CAACGAGTCG
    CACCGCTTGC ATCAGTTCTT CCGTGACATG GATGATGAGG AGTCCTGGAT CAAAGAGAAG
    AAACTGTTGG TGAGCTCAGA GGACTATGGC AGAGACCTGA CTGGTGTGCA GAACCTGAGG
    AAGAAACATA AGCGCTTGGA AGCAGAATTA GCTGCCCATG AACCTGCTAT CCAGGGTGTT
    CTAGACACTG GCAAGAAGCT TTCAGATGAT AACACAATTG GGAAGGAGGA GATACAGCAA
    AGACTGGCTC AGTTTGTGGA CCACTGGAAA GAGTTAAAAC AGCTGGCAGC TGCTCGGGGG
    CAGCGTCTGG AGGAGTCCCT GGAGTACCAA CAGTTTGTAG CAAATGTTGA GGAAGAGGAG
    GCATGGATCA ACGAGAAAAT GACATTGGTA GCCAGTGAGG ATTATGGAGA CACACTTGCT
    GCTATCCAGG GCTTGCTGAA GAAGCATGAA GCATTCGAGA CTGACTTTAC TGTCCACAAA
    GACAGAGTGA ATGATGTTTG CGCTAATGGA GAGGATCTCA TTAAAAAGAA TAATCACCAT
    GTGGAGAACA TTACTGCTAA GATGAAGGGC CTCAAGGGCA AGGTATCAGA TCTGGAGAAA
    GCCGCAGCTC AGAGGAAAGC CAAACTGGAT GAGAACTCTG CCTTCCTCCA GTTCAACTGG
    AAAGCAGATG TGGTGGAGTC ATGGATAGGT GAGAAGGAAA ACAGTCTGAA GACAGATGAT
    TATGGCCGTG ACCTCTCCTC TGTGCAAACA CTACTCACCA AACAGGAAAC CTTTGATGCT
    GGACTTCAGG CTTTCCAGCA GGAGGGAATT GCAAACATCA CTGCTCTGAA AGACCAGCTA
    CTGGCAGCCA AACATATCCA ATCAAAGGCC ATTGAGGTTC GTCACGCTTC CTTGATGAAA
    CGCTGGAATC AGCTGCTTGC TAATTCTGCA GCCAGGAAAA AGAAACTCTT GGAGGCTCAG
    GAGCACTTCA GAAAGGTTGA AGATCTCTTC TTGACTTTTG CCAAGAAGGC CTCTGCCTTC
    AACAGTTGGT TTGAGAATGC TGAGGAGGAC CTGACGGATC CCGTGCGCTG CAATTCCCTG
    GAAGAAATCA AAGCACTGCG AGAAGCCCAC GATGCGTTCC GTTCCTCACT TAGCTCTGCC
    CAAGCTGACT TCAACCAGCT GGCAGAGCTT GATCGGCAGA TCAAGAGCTT CCGTGTAGCC
    TCCAACCCGT ACACTTGGTT TACTATGGAG GCTCTTGAAG AAACTTGGAG GAATCTGCAG
    AAAATTATCA AGGAGCGTGA ATTGGAGTTG CAGAAGGAGC AGCGGAGGCA GGAAGAAAAT
    GACAAGTTGC GCCAGGAGTT TGCTCAGCAT GCTAATGCCT TCCACCAGTG GATTCAGGAG
    ACAAGGACTT ACCTGCTAGA TGGCATAGCC TATCGTCGTG TCATTCGTGT CTATCAGTAT
    GAAGTTGGGG ATGATCTATC TGGAAGGTCC TGCATGGTGG AGGAGTCGGG AACACTGGAA
    TCCCAGCTGG AAGCTACTAA ACGCAAGCAC CAGGAGATCC GGGCTATGAG GAGCCAGCTG
    AAGAAGATTG AGGACCTTGG AGCAGCCATG GAAGAGGCAC TTATCTTGGA CAACAAATAC
    ACAGAGCACA GCACCGTGGG GCTGGCGCAG CAGTGGGACC AGCTTGACCA GCTGGGAATG
    AGAATGCAGC ACAATCTGGA ACAGCAGATT CAAGCTCGAA ACACAACTGG AGTCACAGAG
    GAGGCCCTGA AGGAGTTCAG CATGATGTTC AAGCACTTTG ACAAGGACAA ATCTGGACGT
    CTTAATCACC AGGAGTTTAA GTCTTGCTTG CGCTCCCTTG GCTATGACCT GCCCATGGTT
    GAGGAAGGAG AGCCTGACCC CGAATTTGAG TCTATTCTTG ACACTGTTGA TCCCAACAGG
    GATGGCCACG TCTCGCTGCA GGAGTACATG GCGTTCATGA TCAGCAGGGA AACAGAGAAC
    GTGAAATCCA GCGAGGAGAT TGAGAGTGCT TTCCGTGCCC TCAGCTCGGA GGGGAAGCCT
    TACGTGACCA AGGAGGAGCT CTACCAGAAC CTGACCCGGG AGCAGGCCGA CTACTGCATT
    TCTCACATGA AGCCCTACAT GGATGGCAAG GGCAGAGAAC TTCCTTCTGC CTACGACTAC
    ATAGAATTTA CACGTTCACT CTTTGTGAAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015134773.1
    CDS15..7547
    Translation

    Target ORF information:

    RefSeq Version XM_015279287.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015279287.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
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    1321
    1381
    1441
    1501
    1561
    1621
    1681
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    1921
    1981
    2041
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    2581
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    4441
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    4681
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    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
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    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
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    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
    TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG
    TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC
    TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT
    GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT
    GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG
    ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAA CTCGTCTACA GGAGCTGCAC
    CGACTATGGG AGTTACTGCT GGAAAAAATG AGAGAGAAGG GAGTGAAGCT GTTGCAAGCA
    CAGAAGCTGG TGCAATTCTT ACGGGAATGT GAAGATGTCA TGGACTGGAT CAATGACAAG
    GAAGCAATAG TGACCTCAGA AGAGCTTGGA CAGGACTTAG AGCATGTTGA GGTTTTGCAG
    AAGAAATTTG AAGAGTTCCA AACAGATCTG GCAGCTCATG AGGAGAGAGT GAATGAAGTG
    AACCAGTTCG CTGGCAAACT TATCCAGGAA CAGCACCCTG AGGAGGAACT TATAAAGTCC
    AAACAGGATG AAGTAAATGC AAGCTGGCAG CGTCTTAAGG GGCTTGCCCA ACAGAGGCAA
    GGAAAACTCT TTGGGGCAGC TGAAGTTCAG CGCTTCAACA GGGATGTGGA TGAAACTATC
    AGCTGGATTA AGGAGAAAGG GCAGTTGATG GCCTCAGATG ACTTTGGCAG AGACTTGGCC
    AGCGTGCAAG CTTTACTACG TAAGCATGAA GGCCTGGAAA GAGATCTTGC AGCTTTGGAG
    GATAAGGTTA AGGCCCTCTG TGCAGAAGCT GACCGTTTGC AGCAGTCTCA CCCTATAAAT
    GCTTCTCAAA TTCAAGTGAA ACGGGAGGAA CTGATTGCCA ACTGGGAGCA GATCCGAACT
    CTGGCAGCAG AGAGGCATGC TCGCCTTAAT GACTCCTACA GGTTGCAGCG CTTTCTTGCA
    GATTTCCGGG ACCTCACTAG CTGGGTAACT GAGATGAAGG CTCTGATAAA TGCTGATGAG
    CTTGCCAATG ATGTGGCTGG AGCAGAAGCC CTTCTAGATA GACATCAGGA ACATAAGGGA
    GAAATTGATG CTCATGAGGA TAGCTTCAGA TCTGCTGATG AGTCTGGGCA GGCTTTGCTT
    GCAGCTGGGC ACTATGCTTC TGATGAAGTT AAAGAAAAGC TGACTATCCT CTCAGATGAA
    AGATCAGCCT TGCTGGAACT ATGGGAGCTT CGCAGGCAAC AGTATGAGCA GTGCATGGAT
    CTGCAGCTTT TCTACAGAGA TACTGAACAA GTTGACAACT GGATGAGCAA ACAAGAAGCT
    TTTCTGCTGA ACGAAGACCT TGGTGATTCT CTGGATAGCG TGGAGGCTCT TCTAAAGAAG
    CATGAAGATT TTGAGAAATC CCTAAGTGCT CAAGAGGAGA AGATCACAGC ACTGGATGAG
    TTTGCTACTA AACTGATTCA AAATAACCAT TATGCCATGG ATGACGTTGC TACGCGCAGA
    GATGCTCTTC TGAGTCGCCG AAATGCTCTT CATGAAAGAG CCATGAAGCG CCGTGCCCAA
    CTGGCAGACT CTTTCCATCT CCAGCAGTTT TTCAGGGACT CTGATGAACT GAAGAGTTGG
    GTTAATGAAA AAATGAAAAC TGCTACCGAT GAGGCTTACA AGGATCCATC CAACTTGCAA
    GGTAAAGTTC AGAAGCATCA GGCTTTTGAA GCAGAGCTTT CTGCTAATCA GAGTCGTATT
    GATGCACTGG AGAAAGCTGG CCAGAAGTTG ATTGATGTCA ATCACTATGC ATCTGATGAG
    GTGGCAGCTC GCATGAATGA AGTCATCAGC TTGTGGAAGA AACTTCTGGA AGCCACCGAG
    CTCAAAGGTA TAAAGCTGCG TGAAGCCAAT CAACAGCAGC AATTTAATCG CAATGTAGAA
    GACATTGAGT TGTGGCTGTA TGAAGTGGAG GGTCACTTGG CTTCTGATGA TTATGGAAAA
    GATCTTACCA GCGTTCAGAA TCTTCAGAAG AAACATGCCC TGCTAGAGGC AGATGTTGCT
    GCCCATCAGG ATCGGATAGA TGGCATTACC ATCCAGGCAC GCCAGTTCCA AGATGCTGGG
    CACTTTGATG CTGACAATAT CAAGAAGAAA CAAGAAGCTT TAGTAGCTCG TTATGAAGCT
    CTGAAGGATC CTATGGTGGC TCGCAAGCAG AAACTTGCAG ATTCTCTTCG CCTGCAGCAG
    CTTTTCCGTG ACATCGAGGA TGAAGAGACC TGGATCAGGG AAAAGGAACC TATTGCAGCC
    TCAACAAACC GAGGCAAGGA CTTAATTGGT GTCCAGAATC TGCTAAAGAA GCACCAGGCT
    TTGCAGGCAG AAATTGCAGG CCATGAGCCT CGCATTAAAG CAGTCACACA AAAAGGAAAT
    GCGATGGTGG AAGAGGGACA TTTTGCTGCT GAAGATGTGA AGATCAAATT GAATGAACTA
    AACCAAAAAT GGGACTCTCT GAAAGCCAAG GCATCTCAAC GGCGACAGGA TCTAGAGGAT
    TCTCTGCAAG CTCAGCAGTA TTTTGCTGAT GCTAATGAGG CAGAATCATG GATGAGGGAA
    AAGGAGCCCA TTGTAGGCAG CACGGATTAT GGAAAGGATG AAGACTCTGC TGAGGCTCTC
    CTGAAGAAGC ATGAAGCTTT GATGTCTGAT CTTTCTGCTT ACGGCAGTAG CATACAGGCA
    TTAAGGGAAC AGGCCCAGTC CTGCAGGCAA CAAGTTGCTC CCACTGATGA TGAAACTGGA
    AAAGAGCTTG TGCTAGCACT CTATGATTAC CAAGAGAAGA GTCCTCGGGA GGTGACTATG
    AAGAAAGGAG ATATTCTAAC CCTGCTCAAC AGCACCAACA AGGACTGGTG GAAGGTTGAA
    GTTAACGATC GTCAGGGCTT TGTACCAGCT GCCTATGTGA AAAAACTAGA TCCTGCCCAG
    TCTGCATCCC GAGAGAATCT TCTAGAAGAG CAAGGCAGCA TAGCATTGCG ACAGGAGCAA
    ATTGACAACC AGACTCTCAT TACTAAGGAA GTCGGCAGTG TATCTCTGCG TATGAAACAG
    GTCGAAGAAC TGTATCATTC CCTTCTTGAG CTGGGAGAAA AACGTAAAGG CATGCTAGAA
    AAAAGCTGCA AGAAGTTTAT GCTTTTCCGT GAGGCTAATG AGCTTCAACA GTGGATCAAT
    GAGAAGGAAG CTGCGCTCAC TAATGAGGAA GTGGGTGCTG ATTTGGAGCA GGTGGAGGTG
    CTACAGAAGA AGTTTGATGA TTTTCAGAAG GATTTAAAAG CTAATGAGTC ACGACTGAAG
    GACATAAACA AGGTTGCAAA TGACCTGGAG TCTGAAGGGC TGATGGCAGA GGAAGTGCAA
    GCAGTAGAGC ACCAGGAAGT CTATGGAATG ATGCCCAGAG ATGAAACTGA TTCTAAGACA
    GCCTCTCCTT GGAAGTCTGC ACGTATGATG GTACACACAG TGGCAACGTT TAACTCAATC
    AAGGAACTGA ATGAACGCTG GAGATCCCTG CAGCAGTTGG CAGAGGAGCG AAGCCAGCTG
    TTGGGCAGCG CTCATGAAGT CCAGAGGTTC CACAGAGATG CTGATGAAAC AAAAGAATGG
    ATAGAAGAGA AGAATCAAGC ATTAAATACC GACAACTATG GACATGACTT GGCCAGTGTG
    CAGGCTCTGC AGCGCAAGCA TGAAGGCTTT GAGAGAGACT TGGCAGCTCT TGGAGACAAG
    GTGAATTCTC TTGGTGAAAC TGCCCAGCGT CTGATCCAGT CACATCCAGA ATCAGCTGAA
    GATCTTCAAG AAAAATGTAC TGAGTTGAAC CAAGCCTGGA GTAGTCTGGG AAAACGTGCT
    GACCAACGCA AAGAGAAGCT TGGAGATTCT CATGACCTGC AGCGTTTCCT CAGTGATTTT
    AGAGACCTTA TGTCTTGGAT CAATGGAATC CGGGGTCTGG TCTCCTCAGA TGAACTTGCA
    AAAGATGTGA CTGGAGCTGA AGCTCTGTTG GAAAGGCATC AGGAGCACCG TACTGAAATA
    GATGCACGGG CTGGCACTTT TCAGGCATTT GAACAGTTTG GACAGCAGCT TCTGGCTCGT
    GGACACTATG CCAGCCCAGA GATTAAGGAG AAACTGGATA TTCTAGATCA AGAACGGACA
    GACCTAGAGA AGGCCTGGGT CCAGCGCAGA ATGATGCTAG ACCAGTGCTT AGAACTACAG
    CTGTTTCATC GGGATTGTGA ACAAGCTGAA AACTGGATGG CTGCCCGAGA GGCGTTCCTA
    AATACAGAAG ACAAAGGAGA CTCCTTAGAC AGCGTGGAGG CACTCATCAA GAAGCATGAA
    GATTTCGATA AAGCAATCAA TGTCCAGGAA GAGAAAATTG CTGTCTTGCA GTCTTTTGCT
    GACCAACTGA TTGCTGCTGA TCATTATGCA AAAGGAGTCA TTGCTAACAG ACGCAATGAG
    GTTCTGGACA GGTGGCGTCG TCTGAAGGCT CAGATGATTG AGAAGAGATC TAAGCTGGGA
    GAATCTCAGA CCCTCCAACA GTTCAGTCGT GATGTTGATG AAATAGAAGC TTGGATCAGT
    GAAAAGCTTC AAACTGCAAG TGATGAGTCA TATAAGGATC CCACAAACAT CCAGCTTTCC
    AAACTGCTGA GCAAGCACCA GAAGCACCAA GCCTTTGAAG CTGAGCTCCA CGCCAACGCA
    GATCGGATTC GTGGAGTCAT TGAAATGGGG AACTCTCTTA TTGAAAGAGG AGCGTGTGCT
    GGCAGCGAGG ATGCTGTGAA GGCTCGGCTG GCTGCCCTGG CTGACCAATG GGAGTTCCTG
    GTCCAGAAGT CATCAGAGAA GAGTCAGAAA CTGAAAGAAG CAAATAAACA GCAGAATTTC
    AATACCGGCA TCAAGGACTT TGATTTCTGG CTTTCAGAGG TGGAAGCTTT GTTGGCATCT
    GAAGACTATG GGAAGGACTT GGCATCAGTG AACAACCTTC TGAAGAAGCA CCAATTACTG
    GAAGCTGATA TATCTGCTCA TGAGGATCGG CTGAAGGACC TGAACAGCCA GGCTGACAGT
    TTGATGACCA GCAGTGCTTT CGATACCTCC CAAGTAAAGG ATAAACGTGA AACTATAAAT
    GGGCGCTTCC AGAGAATCAA GAGCATGGCA GCTGCCCGCC GTGCGAAGCT CAACGAGTCG
    CACCGCTTGC ATCAGTTCTT CCGTGACATG GATGATGAGG AGTCCTGGAT CAAAGAGAAG
    AAACTGTTGG TGAGCTCAGA GGACTATGGC AGAGACCTGA CTGGTGTGCA GAACCTGAGG
    AAGAAACATA AGCGCTTGGA AGCAGAATTA GCTGCCCATG AACCTGCTAT CCAGGGTGTT
    CTAGACACTG GCAAGAAGCT TTCAGATGAT AACACAATTG GGAAGGAGGA GATACAGCAA
    AGACTGGCTC AGTTTGTGGA CCACTGGAAA GAGTTAAAAC AGCTGGCAGC TGCTCGGGGG
    CAGCGTCTGG AGGAGTCCCT GGAGTACCAA CAGTTTGTAG CAAATGTTGA GGAAGAGGAG
    GCATGGATCA ACGAGAAAAT GACATTGGTA GCCAGTGAGG ATTATGGAGA CACACTTGCT
    GCTATCCAGG GCTTGCTGAA GAAGCATGAA GCATTCGAGA CTGACTTTAC TGTCCACAAA
    GACAGAGTGA ATGATGTTTG CGCTAATGGA GAGGATCTCA TTAAAAAGAA TAATCACCAT
    GTGGAGAACA TTACTGCTAA GATGAAGGGC CTCAAGGGCA AGGTATCAGA TCTGGAGAAA
    GCCGCAGCTC AGAGGAAAGC CAAACTGGAT GAGAACTCTG CCTTCCTCCA GTTCAACTGG
    AAAGCAGATG TGGTGGAGTC ATGGATAGGT GAGAAGGAAA ACAGTCTGAA GACAGATGAT
    TATGGCCGTG ACCTCTCCTC TGTGCAAACA CTACTCACCA AACAGGAAAC CTTTGATGCT
    GGACTTCAGG CTTTCCAGCA GGAGGGAATT GCAAACATCA CTGCTCTGAA AGACCAGCTA
    CTGGCAGCCA AACATATCCA ATCAAAGGCC ATTGAGGTTC GTCACGCTTC CTTGATGAAA
    CGCTGGAATC AGCTGCTTGC TAATTCTGCA GCCAGGAAAA AGAAACTCTT GGAGGCTCAG
    GAGCACTTCA GAAAGGTTGA AGATCTCTTC TTGACTTTTG CCAAGAAGGC CTCTGCCTTC
    AACAGTTGGT TTGAGAATGC TGAGGAGGAC CTGACGGATC CCGTGCGCTG CAATTCCCTG
    GAAGAAATCA AAGCACTGCG AGAAGCCCAC GATGCGTTCC GTTCCTCACT TAGCTCTGCC
    CAAGCTGACT TCAACCAGCT GGCAGAGCTT GATCGGCAGA TCAAGAGCTT CCGTGTAGCC
    TCCAACCCGT ACACTTGGTT TACTATGGAG GCTCTTGAAG AAACTTGGAG GAATCTGCAG
    AAAATTATCA AGGAGCGTGA ATTGGAGTTG CAGAAGGAGC AGCGGAGGCA GGAAGAAAAT
    GACAAGTTGC GCCAGGAGTT TGCTCAGCAT GCTAATGCCT TCCACCAGTG GATTCAGGAG
    ACAAGGACTT ACCTGCTAGA TGGCATAGCC TATCGTCGTG TCATTCGTGT CTATCAGTAT
    GAAGTTGGGG ATGATCTATC TGGAAGGTCC TGCATGGTGG AGGAGTCGGG AACACTGGAA
    TCCCAGCTGG AAGCTACTAA ACGCAAGCAC CAGGAGATCC GGGCTATGAG GAGCCAGCTG
    AAGAAGATTG AGGACCTTGG AGCAGCCATG GAAGAGGCAC TTATCTTGGA CAACAAATAC
    ACAGAGCACA GCACCGTGGG GCTGGCGCAG CAGTGGGACC AGCTTGACCA GCTGGGAATG
    AGAATGCAGC ACAATCTGGA ACAGCAGATT CAAGCTCGAA ACACAACTGG AGTCACAGAG
    GAGGCCCTGA AGGAGTTCAG CATGATGTTC AAGCACTTTG ACAAGGACAA ATCTGGACGT
    CTTAATCACC AGGAGTTTAA GTCTTGCTTG CGCTCCCTTG GCTATGACCT GCCCATGGTT
    GAGGAAGGAG AGCCTGACCC CGAATTTGAG TCTATTCTTG ACACTGTTGA TCCCAACAGG
    GATGGCCACG TCTCGCTGCA GGAGTACATG GCGTTCATGA TCAGCAGGGA AACAGAGAAC
    GTGAAATCCA GCGAGGAGAT TGAGAGTGCT TTCCGTGCCC TCAGCTCGGA GGGGAAGCCT
    TACGTGACCA AGGAGGAGCT CTACCAGAAC CTGACCCGGG AGCAGGCCGA CTACTGCATT
    TCTCACATGA AGCCCTACAT GGATGGCAAG GGCAGAGAAC TTCCTTCTGC CTACGACTAC
    ATAGAATTTA CACGTTCACT CTTTGTGAAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa43314
    Clone ID Related Accession (Same CDS sequence) XM_015279288.1 , XM_015279288.2
    Accession Version XM_015279288.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7521bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1081
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    1321
    1381
    1441
    1501
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    1621
    1681
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    1981
    2041
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    4081
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    5521
    5581
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    5941
    6001
    6061
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    6181
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    6301
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    6421
    6481
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    6901
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    7261
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    7381
    7441
    7501
    ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
    TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG
    TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC
    TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT
    GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT
    GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG
    ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAT CTTTCACGCC TTACAGGACT
    GCACAGCGCC CAATAGTAAC AGCCACCCAA GTTGGGTCCC ACATACGAAC TCGTCTACAG
    GAGCTGCACC GACTATGGGA GTTACTGCTG GAAAAAATGA GAGAGAAGGG AGTGAAGCTG
    TTGCAAGCAC AGAAGCTGGT GCAATTCTTA CGGGAATGTG AAGATGTCAT GGACTGGATC
    AATGACAAGG AAGCAATAGT GACCTCAGAA GAGCTTGGAC AGGACTTAGA GCATGTTGAG
    GTTTTGCAGA AGAAATTTGA AGAGTTCCAA ACAGATCTGG CAGCTCATGA GGAGAGAGTG
    AATGAAGTGA ACCAGTTCGC TGGCAAACTT ATCCAGGAAC AGCACCCTGA GGAGGAACTT
    ATAAAGTCCA AACAGGATGA AGTAAATGCA AGCTGGCAGC GTCTTAAGGG GCTTGCCCAA
    CAGAGGCAAG GAAAACTCTT TGGGGCAGCT GAAGTTCAGC GCTTCAACAG GGATGTGGAT
    GAAACTATCA GCTGGATTAA GGAGAAAGGG CAGTTGATGG CCTCAGATGA CTTTGGCAGA
    GACTTGGCCA GCGTGCAAGC TTTACTACGT AAGCATGAAG GCCTGGAAAG AGATCTTGCA
    GCTTTGGAGG ATAAGGTTAA GGCCCTCTGT GCAGAAGCTG ACCGTTTGCA GCAGTCTCAC
    CCTATAAATG CTTCTCAAAT TCAAGTGAAA CGGGAGGAAC TGATTGCCAA CTGGGAGCAG
    ATCCGAACTC TGGCAGCAGA GAGGCATGCT CGCCTTAATG ACTCCTACAG GTTGCAGCGC
    TTTCTTGCAG ATTTCCGGGA CCTCACTAGC TGGGTAACTG AGATGAAGGC TCTGATAAAT
    GCTGATGAGC TTGCCAATGA TGTGGCTGGA GCAGAAGCCC TTCTAGATAG ACATCAGGAA
    CATAAGGGAG AAATTGATGC TCATGAGGAT AGCTTCAGAT CTGCTGATGA GTCTGGGCAG
    GCTTTGCTTG CAGCTGGGCA CTATGCTTCT GATGAAGTTA AAGAAAAGCT GACTATCCTC
    TCAGATGAAA GATCAGCCTT GCTGGAACTA TGGGAGCTTC GCAGGCAACA GTATGAGCAG
    TGCATGGATC TGCAGCTTTT CTACAGAGAT ACTGAACAAG TTGACAACTG GATGAGCAAA
    CAAGAAGCTT TTCTGCTGAA CGAAGACCTT GGTGATTCTC TGGATAGCGT GGAGGCTCTT
    CTAAAGAAGC ATGAAGATTT TGAGAAATCC CTAAGTGCTC AAGAGGAGAA GATCACAGCA
    CTGGATGAGT TTGCTACTAA ACTGATTCAA AATAACCATT ATGCCATGGA TGACGTTGCT
    ACGCGCAGAG ATGCTCTTCT GAGTCGCCGA AATGCTCTTC ATGAAAGAGC CATGAAGCGC
    CGTGCCCAAC TGGCAGACTC TTTCCATCTC CAGCAGTTTT TCAGGGACTC TGATGAACTG
    AAGAGTTGGG TTAATGAAAA AATGAAAACT GCTACCGATG AGGCTTACAA GGATCCATCC
    AACTTGCAAG GTAAAGTTCA GAAGCATCAG GCTTTTGAAG CAGAGCTTTC TGCTAATCAG
    AGTCGTATTG ATGCACTGGA GAAAGCTGGC CAGAAGTTGA TTGATGTCAA TCACTATGCA
    TCTGATGAGG TGGCAGCTCG CATGAATGAA GTCATCAGCT TGTGGAAGAA ACTTCTGGAA
    GCCACCGAGC TCAAAGGTAT AAAGCTGCGT GAAGCCAATC AACAGCAGCA ATTTAATCGC
    AATGTAGAAG ACATTGAGTT GTGGCTGTAT GAAGTGGAGG GTCACTTGGC TTCTGATGAT
    TATGGAAAAG ATCTTACCAG CGTTCAGAAT CTTCAGAAGA AACATGCCCT GCTAGAGGCA
    GATGTTGCTG CCCATCAGGA TCGGATAGAT GGCATTACCA TCCAGGCACG CCAGTTCCAA
    GATGCTGGGC ACTTTGATGC TGACAATATC AAGAAGAAAC AAGAAGCTTT AGTAGCTCGT
    TATGAAGCTC TGAAGGATCC TATGGTGGCT CGCAAGCAGA AACTTGCAGA TTCTCTTCGC
    CTGCAGCAGC TTTTCCGTGA CATCGAGGAT GAAGAGACCT GGATCAGGGA AAAGGAACCT
    ATTGCAGCCT CAACAAACCG AGGCAAGGAC TTAATTGGTG TCCAGAATCT GCTAAAGAAG
    CACCAGGCTT TGCAGGCAGA AATTGCAGGC CATGAGCCTC GCATTAAAGC AGTCACACAA
    AAAGGAAATG CGATGGTGGA AGAGGGACAT TTTGCTGCTG AAGATGTGAA GATCAAATTG
    AATGAACTAA ACCAAAAATG GGACTCTCTG AAAGCCAAGG CATCTCAACG GCGACAGGAT
    CTAGAGGATT CTCTGCAAGC TCAGCAGTAT TTTGCTGATG CTAATGAGGC AGAATCATGG
    ATGAGGGAAA AGGAGCCCAT TGTAGGCAGC ACGGATTATG GAAAGGATGA AGACTCTGCT
    GAGGCTCTCC TGAAGAAGCA TGAAGCTTTG ATGTCTGATC TTTCTGCTTA CGGCAGTAGC
    ATACAGGCAT TAAGGGAACA GGCCCAGTCC TGCAGGCAAC AAGTTGCTCC CACTGATGAT
    GAAACTGGAA AAGAGCTTGT GCTAGCACTC TATGATTACC AAGAGAAGAG TCCTCGGGAG
    GTGACTATGA AGAAAGGAGA TATTCTAACC CTGCTCAACA GCACCAACAA GGACTGGTGG
    AAGGTTGAAG TTAACGATCG TCAGGGCTTT GTACCAGCTG CCTATGTGAA AAAACTAGAT
    CCTGCCCAGT CTGCATCCCG AGAGAATCTT CTAGAAGAGC AAGGCAGCAT AGCATTGCGA
    CAGGAGCAAA TTGACAACCA GACTCTCATT ACTAAGGAAG TCGGCAGTGT ATCTCTGCGT
    ATGAAACAGG TCGAAGAACT GTATCATTCC CTTCTTGAGC TGGGAGAAAA ACGTAAAGGC
    ATGCTAGAAA AAAGCTGCAA GAAGTTTATG CTTTTCCGTG AGGCTAATGA GCTTCAACAG
    TGGATCAATG AGAAGGAAGC TGCGCTCACT AATGAGGAAG TGGGTGCTGA TTTGGAGCAG
    GTGGAGGTGC TACAGAAGAA GTTTGATGAT TTTCAGAAGG ATTTAAAAGC TAATGAGTCA
    CGACTGAAGG ACATAAACAA GGTTGCAAAT GACCTGGAGT CTGAAGGGCT GATGGCAGAG
    GAAGTGCAAG CAGTAGAGCA CCAGGAAGTC TATGGAATGA TGCCCAGAGA TGAAACTGAT
    TCTAAGACAG CCTCTCCTTG GAAGTCTGCA CGTATGATGG TACACACAGT GGCAACGTTT
    AACTCAATCA AGGAACTGAA TGAACGCTGG AGATCCCTGC AGCAGTTGGC AGAGGAGCGA
    AGCCAGCTGT TGGGCAGCGC TCATGAAGTC CAGAGGTTCC ACAGAGATGC TGATGAAACA
    AAAGAATGGA TAGAAGAGAA GAATCAAGCA TTAAATACCG ACAACTATGG ACATGACTTG
    GCCAGTGTGC AGGCTCTGCA GCGCAAGCAT GAAGGCTTTG AGAGAGACTT GGCAGCTCTT
    GGAGACAAGG TGAATTCTCT TGGTGAAACT GCCCAGCGTC TGATCCAGTC ACATCCAGAA
    TCAGCTGAAG ATCTTCAAGA AAAATGTACT GAGTTGAACC AAGCCTGGAG TAGTCTGGGA
    AAACGTGCTG ACCAACGCAA AGAGAAGCTT GGAGATTCTC ATGACCTGCA GCGTTTCCTC
    AGTGATTTTA GAGACCTTAT GTCTTGGATC AATGGAATCC GGGGTCTGGT CTCCTCAGAT
    GAACTTGCAA AAGATGTGAC TGGAGCTGAA GCTCTGTTGG AAAGGCATCA GGAGCACCGT
    ACTGAAATAG ATGCACGGGC TGGCACTTTT CAGGCATTTG AACAGTTTGG ACAGCAGCTT
    CTGGCTCGTG GACACTATGC CAGCCCAGAG ATTAAGGAGA AACTGGATAT TCTAGATCAA
    GAACGGACAG ACCTAGAGAA GGCCTGGGTC CAGCGCAGAA TGATGCTAGA CCAGTGCTTA
    GAACTACAGC TGTTTCATCG GGATTGTGAA CAAGCTGAAA ACTGGATGGC TGCCCGAGAG
    GCGTTCCTAA ATACAGAAGA CAAAGGAGAC TCCTTAGACA GCGTGGAGGC ACTCATCAAG
    AAGCATGAAG ATTTCGATAA AGCAATCAAT GTCCAGGAAG AGAAAATTGC TGTCTTGCAG
    TCTTTTGCTG ACCAACTGAT TGCTGCTGAT CATTATGCAA AAGGAGTCAT TGCTAACAGA
    CGCAATGAGG TTCTGGACAG GTGGCGTCGT CTGAAGGCTC AGATGATTGA GAAGAGATCT
    AAGCTGGGAG AATCTCAGAC CCTCCAACAG TTCAGTCGTG ATGTTGATGA AATAGAAGCT
    TGGATCAGTG AAAAGCTTCA AACTGCAAGT GATGAGTCAT ATAAGGATCC CACAAACATC
    CAGCTTTCCA AACTGCTGAG CAAGCACCAG AAGCACCAAG CCTTTGAAGC TGAGCTCCAC
    GCCAACGCAG ATCGGATTCG TGGAGTCATT GAAATGGGGA ACTCTCTTAT TGAAAGAGGA
    GCGTGTGCTG GCAGCGAGGA TGCTGTGAAG GCTCGGCTGG CTGCCCTGGC TGACCAATGG
    GAGTTCCTGG TCCAGAAGTC ATCAGAGAAG AGTCAGAAAC TGAAAGAAGC AAATAAACAG
    CAGAATTTCA ATACCGGCAT CAAGGACTTT GATTTCTGGC TTTCAGAGGT GGAAGCTTTG
    TTGGCATCTG AAGACTATGG GAAGGACTTG GCATCAGTGA ACAACCTTCT GAAGAAGCAC
    CAATTACTGG AAGCTGATAT ATCTGCTCAT GAGGATCGGC TGAAGGACCT GAACAGCCAG
    GCTGACAGTT TGATGACCAG CAGTGCTTTC GATACCTCCC AAGTAAAGGA TAAACGTGAA
    ACTATAAATG GGCGCTTCCA GAGAATCAAG AGCATGGCAG CTGCCCGCCG TGCGAAGCTC
    AACGAGTCGC ACCGCTTGCA TCAGTTCTTC CGTGACATGG ATGATGAGGA GTCCTGGATC
    AAAGAGAAGA AACTGTTGGT GAGCTCAGAG GACTATGGCA GAGACCTGAC TGGTGTGCAG
    AACCTGAGGA AGAAACATAA GCGCTTGGAA GCAGAATTAG CTGCCCATGA ACCTGCTATC
    CAGGGTGTTC TAGACACTGG CAAGAAGCTT TCAGATGATA ACACAATTGG GAAGGAGGAG
    ATACAGCAAA GACTGGCTCA GTTTGTGGAC CACTGGAAAG AGTTAAAACA GCTGGCAGCT
    GCTCGGGGGC AGCGTCTGGA GGAGTCCCTG GAGTACCAAC AGTTTGTAGC AAATGTTGAG
    GAAGAGGAGG CATGGATCAA CGAGAAAATG ACATTGGTAG CCAGTGAGGA TTATGGAGAC
    ACACTTGCTG CTATCCAGGG CTTGCTGAAG AAGCATGAAG CATTCGAGAC TGACTTTACT
    GTCCACAAAG ACAGAGTGAA TGATGTTTGC GCTAATGGAG AGGATCTCAT TAAAAAGAAT
    AATCACCATG TGGAGAACAT TACTGCTAAG ATGAAGGGCC TCAAGGGCAA GGTATCAGAT
    CTGGAGAAAG CCGCAGCTCA GAGGAAAGCC AAACTGGATG AGAACTCTGC CTTCCTCCAG
    TTCAACTGGA AAGCAGATGT GGTGGAGTCA TGGATAGGTG AGAAGGAAAA CAGTCTGAAG
    ACAGATGATT ATGGCCGTGA CCTCTCCTCT GTGCAAACAC TACTCACCAA ACAGGAAACC
    TTTGATGCTG GACTTCAGGC TTTCCAGCAG GAGGGAATTG CAAACATCAC TGCTCTGAAA
    GACCAGCTAC TGGCAGCCAA ACATATCCAA TCAAAGGCCA TTGAGGTTCG TCACGCTTCC
    TTGATGAAAC GCTGGAATCA GCTGCTTGCT AATTCTGCAG CCAGGAAAAA GAAACTCTTG
    GAGGCTCAGG AGCACTTCAG AAAGGTTGAA GATCTCTTCT TGACTTTTGC CAAGAAGGCC
    TCTGCCTTCA ACAGTTGGTT TGAGAATGCT GAGGAGGACC TGACGGATCC CGTGCGCTGC
    AATTCCCTGG AAGAAATCAA AGCACTGCGA GAAGCCCACG ATGCGTTCCG TTCCTCACTT
    AGCTCTGCCC AAGCTGACTT CAACCAGCTG GCAGAGCTTG ATCGGCAGAT CAAGAGCTTC
    CGTGTAGCCT CCAACCCGTA CACTTGGTTT ACTATGGAGG CTCTTGAAGA AACTTGGAGG
    AATCTGCAGA AAATTATCAA GGAGCGTGAA TTGGAGTTGC AGAAGGAGCA GCGGAGGCAG
    GAAGAAAATG ACAAGTTGCG CCAGGAGTTT GCTCAGCATG CTAATGCCTT CCACCAGTGG
    ATTCAGGAGA CAAGGTCCTG CATGGTGGAG GAGTCGGGAA CACTGGAATC CCAGCTGGAA
    GCTACTAAAC GCAAGCACCA GGAGATCCGG GCTATGAGGA GCCAGCTGAA GAAGATTGAG
    GACCTTGGAG CAGCCATGGA AGAGGCACTT ATCTTGGACA ACAAATACAC AGAGCACAGC
    ACCGTGGGGC TGGCGCAGCA GTGGGACCAG CTTGACCAGC TGGGAATGAG AATGCAGCAC
    AATCTGGAAC AGCAGATTCA AGCTCGAAAC ACAACTGGAG TCACAGAGGA GGCCCTGAAG
    GAGTTCAGCA TGATGTTCAA GCACTTTGAC AAGGACAAAT CTGGACGTCT TAATCACCAG
    GAGTTTAAGT CTTGCTTGCG CTCCCTTGGC TATGACCTGC CCATGGTTGA GGAAGGAGAG
    CCTGACCCCG AATTTGAGTC TATTCTTGAC ACTGTTGATC CCAACAGGGA TGGCCACGTC
    TCGCTGCAGG AGTACATGGC GTTCATGATC AGCAGGGAAA CAGAGAACGT GAAATCCAGC
    GAGGAGATTG AGAGTGCTTT CCGTGCCCTC AGCTCGGAGG GGAAGCCTTA CGTGACCAAG
    GAGGAGCTCT ACCAGAACCT GACCCGGGAG CAGGCCGACT ACTGCATTTC TCACATGAAG
    CCCTACATGG ATGGCAAGGG CAGAGAACTT CCTTCTGCCT ACGACTACAT AGAATTTACA
    CGTTCACTCT TTGTGAATTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015134774.1
    CDS17..7537
    Translation

    Target ORF information:

    RefSeq Version XM_015279288.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015279288.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
    TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG
    TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC
    TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT
    GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT
    GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG
    ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAT CTTTCACGCC TTACAGGACT
    GCACAGCGCC CAATAGTAAC AGCCACCCAA GTTGGGTCCC ACATACGAAC TCGTCTACAG
    GAGCTGCACC GACTATGGGA GTTACTGCTG GAAAAAATGA GAGAGAAGGG AGTGAAGCTG
    TTGCAAGCAC AGAAGCTGGT GCAATTCTTA CGGGAATGTG AAGATGTCAT GGACTGGATC
    AATGACAAGG AAGCAATAGT GACCTCAGAA GAGCTTGGAC AGGACTTAGA GCATGTTGAG
    GTTTTGCAGA AGAAATTTGA AGAGTTCCAA ACAGATCTGG CAGCTCATGA GGAGAGAGTG
    AATGAAGTGA ACCAGTTCGC TGGCAAACTT ATCCAGGAAC AGCACCCTGA GGAGGAACTT
    ATAAAGTCCA AACAGGATGA AGTAAATGCA AGCTGGCAGC GTCTTAAGGG GCTTGCCCAA
    CAGAGGCAAG GAAAACTCTT TGGGGCAGCT GAAGTTCAGC GCTTCAACAG GGATGTGGAT
    GAAACTATCA GCTGGATTAA GGAGAAAGGG CAGTTGATGG CCTCAGATGA CTTTGGCAGA
    GACTTGGCCA GCGTGCAAGC TTTACTACGT AAGCATGAAG GCCTGGAAAG AGATCTTGCA
    GCTTTGGAGG ATAAGGTTAA GGCCCTCTGT GCAGAAGCTG ACCGTTTGCA GCAGTCTCAC
    CCTATAAATG CTTCTCAAAT TCAAGTGAAA CGGGAGGAAC TGATTGCCAA CTGGGAGCAG
    ATCCGAACTC TGGCAGCAGA GAGGCATGCT CGCCTTAATG ACTCCTACAG GTTGCAGCGC
    TTTCTTGCAG ATTTCCGGGA CCTCACTAGC TGGGTAACTG AGATGAAGGC TCTGATAAAT
    GCTGATGAGC TTGCCAATGA TGTGGCTGGA GCAGAAGCCC TTCTAGATAG ACATCAGGAA
    CATAAGGGAG AAATTGATGC TCATGAGGAT AGCTTCAGAT CTGCTGATGA GTCTGGGCAG
    GCTTTGCTTG CAGCTGGGCA CTATGCTTCT GATGAAGTTA AAGAAAAGCT GACTATCCTC
    TCAGATGAAA GATCAGCCTT GCTGGAACTA TGGGAGCTTC GCAGGCAACA GTATGAGCAG
    TGCATGGATC TGCAGCTTTT CTACAGAGAT ACTGAACAAG TTGACAACTG GATGAGCAAA
    CAAGAAGCTT TTCTGCTGAA CGAAGACCTT GGTGATTCTC TGGATAGCGT GGAGGCTCTT
    CTAAAGAAGC ATGAAGATTT TGAGAAATCC CTAAGTGCTC AAGAGGAGAA GATCACAGCA
    CTGGATGAGT TTGCTACTAA ACTGATTCAA AATAACCATT ATGCCATGGA TGACGTTGCT
    ACGCGCAGAG ATGCTCTTCT GAGTCGCCGA AATGCTCTTC ATGAAAGAGC CATGAAGCGC
    CGTGCCCAAC TGGCAGACTC TTTCCATCTC CAGCAGTTTT TCAGGGACTC TGATGAACTG
    AAGAGTTGGG TTAATGAAAA AATGAAAACT GCTACCGATG AGGCTTACAA GGATCCATCC
    AACTTGCAAG GTAAAGTTCA GAAGCATCAG GCTTTTGAAG CAGAGCTTTC TGCTAATCAG
    AGTCGTATTG ATGCACTGGA GAAAGCTGGC CAGAAGTTGA TTGATGTCAA TCACTATGCA
    TCTGATGAGG TGGCAGCTCG CATGAATGAA GTCATCAGCT TGTGGAAGAA ACTTCTGGAA
    GCCACCGAGC TCAAAGGTAT AAAGCTGCGT GAAGCCAATC AACAGCAGCA ATTTAATCGC
    AATGTAGAAG ACATTGAGTT GTGGCTGTAT GAAGTGGAGG GTCACTTGGC TTCTGATGAT
    TATGGAAAAG ATCTTACCAG CGTTCAGAAT CTTCAGAAGA AACATGCCCT GCTAGAGGCA
    GATGTTGCTG CCCATCAGGA TCGGATAGAT GGCATTACCA TCCAGGCACG CCAGTTCCAA
    GATGCTGGGC ACTTTGATGC TGACAATATC AAGAAGAAAC AAGAAGCTTT AGTAGCTCGT
    TATGAAGCTC TGAAGGATCC TATGGTGGCT CGCAAGCAGA AACTTGCAGA TTCTCTTCGC
    CTGCAGCAGC TTTTCCGTGA CATCGAGGAT GAAGAGACCT GGATCAGGGA AAAGGAACCT
    ATTGCAGCCT CAACAAACCG AGGCAAGGAC TTAATTGGTG TCCAGAATCT GCTAAAGAAG
    CACCAGGCTT TGCAGGCAGA AATTGCAGGC CATGAGCCTC GCATTAAAGC AGTCACACAA
    AAAGGAAATG CGATGGTGGA AGAGGGACAT TTTGCTGCTG AAGATGTGAA GATCAAATTG
    AATGAACTAA ACCAAAAATG GGACTCTCTG AAAGCCAAGG CATCTCAACG GCGACAGGAT
    CTAGAGGATT CTCTGCAAGC TCAGCAGTAT TTTGCTGATG CTAATGAGGC AGAATCATGG
    ATGAGGGAAA AGGAGCCCAT TGTAGGCAGC ACGGATTATG GAAAGGATGA AGACTCTGCT
    GAGGCTCTCC TGAAGAAGCA TGAAGCTTTG ATGTCTGATC TTTCTGCTTA CGGCAGTAGC
    ATACAGGCAT TAAGGGAACA GGCCCAGTCC TGCAGGCAAC AAGTTGCTCC CACTGATGAT
    GAAACTGGAA AAGAGCTTGT GCTAGCACTC TATGATTACC AAGAGAAGAG TCCTCGGGAG
    GTGACTATGA AGAAAGGAGA TATTCTAACC CTGCTCAACA GCACCAACAA GGACTGGTGG
    AAGGTTGAAG TTAACGATCG TCAGGGCTTT GTACCAGCTG CCTATGTGAA AAAACTAGAT
    CCTGCCCAGT CTGCATCCCG AGAGAATCTT CTAGAAGAGC AAGGCAGCAT AGCATTGCGA
    CAGGAGCAAA TTGACAACCA GACTCTCATT ACTAAGGAAG TCGGCAGTGT ATCTCTGCGT
    ATGAAACAGG TCGAAGAACT GTATCATTCC CTTCTTGAGC TGGGAGAAAA ACGTAAAGGC
    ATGCTAGAAA AAAGCTGCAA GAAGTTTATG CTTTTCCGTG AGGCTAATGA GCTTCAACAG
    TGGATCAATG AGAAGGAAGC TGCGCTCACT AATGAGGAAG TGGGTGCTGA TTTGGAGCAG
    GTGGAGGTGC TACAGAAGAA GTTTGATGAT TTTCAGAAGG ATTTAAAAGC TAATGAGTCA
    CGACTGAAGG ACATAAACAA GGTTGCAAAT GACCTGGAGT CTGAAGGGCT GATGGCAGAG
    GAAGTGCAAG CAGTAGAGCA CCAGGAAGTC TATGGAATGA TGCCCAGAGA TGAAACTGAT
    TCTAAGACAG CCTCTCCTTG GAAGTCTGCA CGTATGATGG TACACACAGT GGCAACGTTT
    AACTCAATCA AGGAACTGAA TGAACGCTGG AGATCCCTGC AGCAGTTGGC AGAGGAGCGA
    AGCCAGCTGT TGGGCAGCGC TCATGAAGTC CAGAGGTTCC ACAGAGATGC TGATGAAACA
    AAAGAATGGA TAGAAGAGAA GAATCAAGCA TTAAATACCG ACAACTATGG ACATGACTTG
    GCCAGTGTGC AGGCTCTGCA GCGCAAGCAT GAAGGCTTTG AGAGAGACTT GGCAGCTCTT
    GGAGACAAGG TGAATTCTCT TGGTGAAACT GCCCAGCGTC TGATCCAGTC ACATCCAGAA
    TCAGCTGAAG ATCTTCAAGA AAAATGTACT GAGTTGAACC AAGCCTGGAG TAGTCTGGGA
    AAACGTGCTG ACCAACGCAA AGAGAAGCTT GGAGATTCTC ATGACCTGCA GCGTTTCCTC
    AGTGATTTTA GAGACCTTAT GTCTTGGATC AATGGAATCC GGGGTCTGGT CTCCTCAGAT
    GAACTTGCAA AAGATGTGAC TGGAGCTGAA GCTCTGTTGG AAAGGCATCA GGAGCACCGT
    ACTGAAATAG ATGCACGGGC TGGCACTTTT CAGGCATTTG AACAGTTTGG ACAGCAGCTT
    CTGGCTCGTG GACACTATGC CAGCCCAGAG ATTAAGGAGA AACTGGATAT TCTAGATCAA
    GAACGGACAG ACCTAGAGAA GGCCTGGGTC CAGCGCAGAA TGATGCTAGA CCAGTGCTTA
    GAACTACAGC TGTTTCATCG GGATTGTGAA CAAGCTGAAA ACTGGATGGC TGCCCGAGAG
    GCGTTCCTAA ATACAGAAGA CAAAGGAGAC TCCTTAGACA GCGTGGAGGC ACTCATCAAG
    AAGCATGAAG ATTTCGATAA AGCAATCAAT GTCCAGGAAG AGAAAATTGC TGTCTTGCAG
    TCTTTTGCTG ACCAACTGAT TGCTGCTGAT CATTATGCAA AAGGAGTCAT TGCTAACAGA
    CGCAATGAGG TTCTGGACAG GTGGCGTCGT CTGAAGGCTC AGATGATTGA GAAGAGATCT
    AAGCTGGGAG AATCTCAGAC CCTCCAACAG TTCAGTCGTG ATGTTGATGA AATAGAAGCT
    TGGATCAGTG AAAAGCTTCA AACTGCAAGT GATGAGTCAT ATAAGGATCC CACAAACATC
    CAGCTTTCCA AACTGCTGAG CAAGCACCAG AAGCACCAAG CCTTTGAAGC TGAGCTCCAC
    GCCAACGCAG ATCGGATTCG TGGAGTCATT GAAATGGGGA ACTCTCTTAT TGAAAGAGGA
    GCGTGTGCTG GCAGCGAGGA TGCTGTGAAG GCTCGGCTGG CTGCCCTGGC TGACCAATGG
    GAGTTCCTGG TCCAGAAGTC ATCAGAGAAG AGTCAGAAAC TGAAAGAAGC AAATAAACAG
    CAGAATTTCA ATACCGGCAT CAAGGACTTT GATTTCTGGC TTTCAGAGGT GGAAGCTTTG
    TTGGCATCTG AAGACTATGG GAAGGACTTG GCATCAGTGA ACAACCTTCT GAAGAAGCAC
    CAATTACTGG AAGCTGATAT ATCTGCTCAT GAGGATCGGC TGAAGGACCT GAACAGCCAG
    GCTGACAGTT TGATGACCAG CAGTGCTTTC GATACCTCCC AAGTAAAGGA TAAACGTGAA
    ACTATAAATG GGCGCTTCCA GAGAATCAAG AGCATGGCAG CTGCCCGCCG TGCGAAGCTC
    AACGAGTCGC ACCGCTTGCA TCAGTTCTTC CGTGACATGG ATGATGAGGA GTCCTGGATC
    AAAGAGAAGA AACTGTTGGT GAGCTCAGAG GACTATGGCA GAGACCTGAC TGGTGTGCAG
    AACCTGAGGA AGAAACATAA GCGCTTGGAA GCAGAATTAG CTGCCCATGA ACCTGCTATC
    CAGGGTGTTC TAGACACTGG CAAGAAGCTT TCAGATGATA ACACAATTGG GAAGGAGGAG
    ATACAGCAAA GACTGGCTCA GTTTGTGGAC CACTGGAAAG AGTTAAAACA GCTGGCAGCT
    GCTCGGGGGC AGCGTCTGGA GGAGTCCCTG GAGTACCAAC AGTTTGTAGC AAATGTTGAG
    GAAGAGGAGG CATGGATCAA CGAGAAAATG ACATTGGTAG CCAGTGAGGA TTATGGAGAC
    ACACTTGCTG CTATCCAGGG CTTGCTGAAG AAGCATGAAG CATTCGAGAC TGACTTTACT
    GTCCACAAAG ACAGAGTGAA TGATGTTTGC GCTAATGGAG AGGATCTCAT TAAAAAGAAT
    AATCACCATG TGGAGAACAT TACTGCTAAG ATGAAGGGCC TCAAGGGCAA GGTATCAGAT
    CTGGAGAAAG CCGCAGCTCA GAGGAAAGCC AAACTGGATG AGAACTCTGC CTTCCTCCAG
    TTCAACTGGA AAGCAGATGT GGTGGAGTCA TGGATAGGTG AGAAGGAAAA CAGTCTGAAG
    ACAGATGATT ATGGCCGTGA CCTCTCCTCT GTGCAAACAC TACTCACCAA ACAGGAAACC
    TTTGATGCTG GACTTCAGGC TTTCCAGCAG GAGGGAATTG CAAACATCAC TGCTCTGAAA
    GACCAGCTAC TGGCAGCCAA ACATATCCAA TCAAAGGCCA TTGAGGTTCG TCACGCTTCC
    TTGATGAAAC GCTGGAATCA GCTGCTTGCT AATTCTGCAG CCAGGAAAAA GAAACTCTTG
    GAGGCTCAGG AGCACTTCAG AAAGGTTGAA GATCTCTTCT TGACTTTTGC CAAGAAGGCC
    TCTGCCTTCA ACAGTTGGTT TGAGAATGCT GAGGAGGACC TGACGGATCC CGTGCGCTGC
    AATTCCCTGG AAGAAATCAA AGCACTGCGA GAAGCCCACG ATGCGTTCCG TTCCTCACTT
    AGCTCTGCCC AAGCTGACTT CAACCAGCTG GCAGAGCTTG ATCGGCAGAT CAAGAGCTTC
    CGTGTAGCCT CCAACCCGTA CACTTGGTTT ACTATGGAGG CTCTTGAAGA AACTTGGAGG
    AATCTGCAGA AAATTATCAA GGAGCGTGAA TTGGAGTTGC AGAAGGAGCA GCGGAGGCAG
    GAAGAAAATG ACAAGTTGCG CCAGGAGTTT GCTCAGCATG CTAATGCCTT CCACCAGTGG
    ATTCAGGAGA CAAGGTCCTG CATGGTGGAG GAGTCGGGAA CACTGGAATC CCAGCTGGAA
    GCTACTAAAC GCAAGCACCA GGAGATCCGG GCTATGAGGA GCCAGCTGAA GAAGATTGAG
    GACCTTGGAG CAGCCATGGA AGAGGCACTT ATCTTGGACA ACAAATACAC AGAGCACAGC
    ACCGTGGGGC TGGCGCAGCA GTGGGACCAG CTTGACCAGC TGGGAATGAG AATGCAGCAC
    AATCTGGAAC AGCAGATTCA AGCTCGAAAC ACAACTGGAG TCACAGAGGA GGCCCTGAAG
    GAGTTCAGCA TGATGTTCAA GCACTTTGAC AAGGACAAAT CTGGACGTCT TAATCACCAG
    GAGTTTAAGT CTTGCTTGCG CTCCCTTGGC TATGACCTGC CCATGGTTGA GGAAGGAGAG
    CCTGACCCCG AATTTGAGTC TATTCTTGAC ACTGTTGATC CCAACAGGGA TGGCCACGTC
    TCGCTGCAGG AGTACATGGC GTTCATGATC AGCAGGGAAA CAGAGAACGT GAAATCCAGC
    GAGGAGATTG AGAGTGCTTT CCGTGCCCTC AGCTCGGAGG GGAAGCCTTA CGTGACCAAG
    GAGGAGCTCT ACCAGAACCT GACCCGGGAG CAGGCCGACT ACTGCATTTC TCACATGAAG
    CCCTACATGG ATGGCAAGGG CAGAGAACTT CCTTCTGCCT ACGACTACAT AGAATTTACA
    CGTTCACTCT TTGTGAATTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa43314
    Clone ID Related Accession (Same CDS sequence) XM_015279288.1 , XM_015279288.2
    Accession Version XM_015279288.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7521bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015279288.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
    TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG
    TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC
    TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT
    GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT
    GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG
    ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAT CTTTCACGCC TTACAGGACT
    GCACAGCGCC CAATAGTAAC AGCCACCCAA GTTGGGTCCC ACATACGAAC TCGTCTACAG
    GAGCTGCACC GACTATGGGA GTTACTGCTG GAAAAAATGA GAGAGAAGGG AGTGAAGCTG
    TTGCAAGCAC AGAAGCTGGT GCAATTCTTA CGGGAATGTG AAGATGTCAT GGACTGGATC
    AATGACAAGG AAGCAATAGT GACCTCAGAA GAGCTTGGAC AGGACTTAGA GCATGTTGAG
    GTTTTGCAGA AGAAATTTGA AGAGTTCCAA ACAGATCTGG CAGCTCATGA GGAGAGAGTG
    AATGAAGTGA ACCAGTTCGC TGGCAAACTT ATCCAGGAAC AGCACCCTGA GGAGGAACTT
    ATAAAGTCCA AACAGGATGA AGTAAATGCA AGCTGGCAGC GTCTTAAGGG GCTTGCCCAA
    CAGAGGCAAG GAAAACTCTT TGGGGCAGCT GAAGTTCAGC GCTTCAACAG GGATGTGGAT
    GAAACTATCA GCTGGATTAA GGAGAAAGGG CAGTTGATGG CCTCAGATGA CTTTGGCAGA
    GACTTGGCCA GCGTGCAAGC TTTACTACGT AAGCATGAAG GCCTGGAAAG AGATCTTGCA
    GCTTTGGAGG ATAAGGTTAA GGCCCTCTGT GCAGAAGCTG ACCGTTTGCA GCAGTCTCAC
    CCTATAAATG CTTCTCAAAT TCAAGTGAAA CGGGAGGAAC TGATTGCCAA CTGGGAGCAG
    ATCCGAACTC TGGCAGCAGA GAGGCATGCT CGCCTTAATG ACTCCTACAG GTTGCAGCGC
    TTTCTTGCAG ATTTCCGGGA CCTCACTAGC TGGGTAACTG AGATGAAGGC TCTGATAAAT
    GCTGATGAGC TTGCCAATGA TGTGGCTGGA GCAGAAGCCC TTCTAGATAG ACATCAGGAA
    CATAAGGGAG AAATTGATGC TCATGAGGAT AGCTTCAGAT CTGCTGATGA GTCTGGGCAG
    GCTTTGCTTG CAGCTGGGCA CTATGCTTCT GATGAAGTTA AAGAAAAGCT GACTATCCTC
    TCAGATGAAA GATCAGCCTT GCTGGAACTA TGGGAGCTTC GCAGGCAACA GTATGAGCAG
    TGCATGGATC TGCAGCTTTT CTACAGAGAT ACTGAACAAG TTGACAACTG GATGAGCAAA
    CAAGAAGCTT TTCTGCTGAA CGAAGACCTT GGTGATTCTC TGGATAGCGT GGAGGCTCTT
    CTAAAGAAGC ATGAAGATTT TGAGAAATCC CTAAGTGCTC AAGAGGAGAA GATCACAGCA
    CTGGATGAGT TTGCTACTAA ACTGATTCAA AATAACCATT ATGCCATGGA TGACGTTGCT
    ACGCGCAGAG ATGCTCTTCT GAGTCGCCGA AATGCTCTTC ATGAAAGAGC CATGAAGCGC
    CGTGCCCAAC TGGCAGACTC TTTCCATCTC CAGCAGTTTT TCAGGGACTC TGATGAACTG
    AAGAGTTGGG TTAATGAAAA AATGAAAACT GCTACCGATG AGGCTTACAA GGATCCATCC
    AACTTGCAAG GTAAAGTTCA GAAGCATCAG GCTTTTGAAG CAGAGCTTTC TGCTAATCAG
    AGTCGTATTG ATGCACTGGA GAAAGCTGGC CAGAAGTTGA TTGATGTCAA TCACTATGCA
    TCTGATGAGG TGGCAGCTCG CATGAATGAA GTCATCAGCT TGTGGAAGAA ACTTCTGGAA
    GCCACCGAGC TCAAAGGTAT AAAGCTGCGT GAAGCCAATC AACAGCAGCA ATTTAATCGC
    AATGTAGAAG ACATTGAGTT GTGGCTGTAT GAAGTGGAGG GTCACTTGGC TTCTGATGAT
    TATGGAAAAG ATCTTACCAG CGTTCAGAAT CTTCAGAAGA AACATGCCCT GCTAGAGGCA
    GATGTTGCTG CCCATCAGGA TCGGATAGAT GGCATTACCA TCCAGGCACG CCAGTTCCAA
    GATGCTGGGC ACTTTGATGC TGACAATATC AAGAAGAAAC AAGAAGCTTT AGTAGCTCGT
    TATGAAGCTC TGAAGGATCC TATGGTGGCT CGCAAGCAGA AACTTGCAGA TTCTCTTCGC
    CTGCAGCAGC TTTTCCGTGA CATCGAGGAT GAAGAGACCT GGATCAGGGA AAAGGAACCT
    ATTGCAGCCT CAACAAACCG AGGCAAGGAC TTAATTGGTG TCCAGAATCT GCTAAAGAAG
    CACCAGGCTT TGCAGGCAGA AATTGCAGGC CATGAGCCTC GCATTAAAGC AGTCACACAA
    AAAGGAAATG CGATGGTGGA AGAGGGACAT TTTGCTGCTG AAGATGTGAA GATCAAATTG
    AATGAACTAA ACCAAAAATG GGACTCTCTG AAAGCCAAGG CATCTCAACG GCGACAGGAT
    CTAGAGGATT CTCTGCAAGC TCAGCAGTAT TTTGCTGATG CTAATGAGGC AGAATCATGG
    ATGAGGGAAA AGGAGCCCAT TGTAGGCAGC ACGGATTATG GAAAGGATGA AGACTCTGCT
    GAGGCTCTCC TGAAGAAGCA TGAAGCTTTG ATGTCTGATC TTTCTGCTTA CGGCAGTAGC
    ATACAGGCAT TAAGGGAACA GGCCCAGTCC TGCAGGCAAC AAGTTGCTCC CACTGATGAT
    GAAACTGGAA AAGAGCTTGT GCTAGCACTC TATGATTACC AAGAGAAGAG TCCTCGGGAG
    GTGACTATGA AGAAAGGAGA TATTCTAACC CTGCTCAACA GCACCAACAA GGACTGGTGG
    AAGGTTGAAG TTAACGATCG TCAGGGCTTT GTACCAGCTG CCTATGTGAA AAAACTAGAT
    CCTGCCCAGT CTGCATCCCG AGAGAATCTT CTAGAAGAGC AAGGCAGCAT AGCATTGCGA
    CAGGAGCAAA TTGACAACCA GACTCTCATT ACTAAGGAAG TCGGCAGTGT ATCTCTGCGT
    ATGAAACAGG TCGAAGAACT GTATCATTCC CTTCTTGAGC TGGGAGAAAA ACGTAAAGGC
    ATGCTAGAAA AAAGCTGCAA GAAGTTTATG CTTTTCCGTG AGGCTAATGA GCTTCAACAG
    TGGATCAATG AGAAGGAAGC TGCGCTCACT AATGAGGAAG TGGGTGCTGA TTTGGAGCAG
    GTGGAGGTGC TACAGAAGAA GTTTGATGAT TTTCAGAAGG ATTTAAAAGC TAATGAGTCA
    CGACTGAAGG ACATAAACAA GGTTGCAAAT GACCTGGAGT CTGAAGGGCT GATGGCAGAG
    GAAGTGCAAG CAGTAGAGCA CCAGGAAGTC TATGGAATGA TGCCCAGAGA TGAAACTGAT
    TCTAAGACAG CCTCTCCTTG GAAGTCTGCA CGTATGATGG TACACACAGT GGCAACGTTT
    AACTCAATCA AGGAACTGAA TGAACGCTGG AGATCCCTGC AGCAGTTGGC AGAGGAGCGA
    AGCCAGCTGT TGGGCAGCGC TCATGAAGTC CAGAGGTTCC ACAGAGATGC TGATGAAACA
    AAAGAATGGA TAGAAGAGAA GAATCAAGCA TTAAATACCG ACAACTATGG ACATGACTTG
    GCCAGTGTGC AGGCTCTGCA GCGCAAGCAT GAAGGCTTTG AGAGAGACTT GGCAGCTCTT
    GGAGACAAGG TGAATTCTCT TGGTGAAACT GCCCAGCGTC TGATCCAGTC ACATCCAGAA
    TCAGCTGAAG ATCTTCAAGA AAAATGTACT GAGTTGAACC AAGCCTGGAG TAGTCTGGGA
    AAACGTGCTG ACCAACGCAA AGAGAAGCTT GGAGATTCTC ATGACCTGCA GCGTTTCCTC
    AGTGATTTTA GAGACCTTAT GTCTTGGATC AATGGAATCC GGGGTCTGGT CTCCTCAGAT
    GAACTTGCAA AAGATGTGAC TGGAGCTGAA GCTCTGTTGG AAAGGCATCA GGAGCACCGT
    ACTGAAATAG ATGCACGGGC TGGCACTTTT CAGGCATTTG AACAGTTTGG ACAGCAGCTT
    CTGGCTCGTG GACACTATGC CAGCCCAGAG ATTAAGGAGA AACTGGATAT TCTAGATCAA
    GAACGGACAG ACCTAGAGAA GGCCTGGGTC CAGCGCAGAA TGATGCTAGA CCAGTGCTTA
    GAACTACAGC TGTTTCATCG GGATTGTGAA CAAGCTGAAA ACTGGATGGC TGCCCGAGAG
    GCGTTCCTAA ATACAGAAGA CAAAGGAGAC TCCTTAGACA GCGTGGAGGC ACTCATCAAG
    AAGCATGAAG ATTTCGATAA AGCAATCAAT GTCCAGGAAG AGAAAATTGC TGTCTTGCAG
    TCTTTTGCTG ACCAACTGAT TGCTGCTGAT CATTATGCAA AAGGAGTCAT TGCTAACAGA
    CGCAATGAGG TTCTGGACAG GTGGCGTCGT CTGAAGGCTC AGATGATTGA GAAGAGATCT
    AAGCTGGGAG AATCTCAGAC CCTCCAACAG TTCAGTCGTG ATGTTGATGA AATAGAAGCT
    TGGATCAGTG AAAAGCTTCA AACTGCAAGT GATGAGTCAT ATAAGGATCC CACAAACATC
    CAGCTTTCCA AACTGCTGAG CAAGCACCAG AAGCACCAAG CCTTTGAAGC TGAGCTCCAC
    GCCAACGCAG ATCGGATTCG TGGAGTCATT GAAATGGGGA ACTCTCTTAT TGAAAGAGGA
    GCGTGTGCTG GCAGCGAGGA TGCTGTGAAG GCTCGGCTGG CTGCCCTGGC TGACCAATGG
    GAGTTCCTGG TCCAGAAGTC ATCAGAGAAG AGTCAGAAAC TGAAAGAAGC AAATAAACAG
    CAGAATTTCA ATACCGGCAT CAAGGACTTT GATTTCTGGC TTTCAGAGGT GGAAGCTTTG
    TTGGCATCTG AAGACTATGG GAAGGACTTG GCATCAGTGA ACAACCTTCT GAAGAAGCAC
    CAATTACTGG AAGCTGATAT ATCTGCTCAT GAGGATCGGC TGAAGGACCT GAACAGCCAG
    GCTGACAGTT TGATGACCAG CAGTGCTTTC GATACCTCCC AAGTAAAGGA TAAACGTGAA
    ACTATAAATG GGCGCTTCCA GAGAATCAAG AGCATGGCAG CTGCCCGCCG TGCGAAGCTC
    AACGAGTCGC ACCGCTTGCA TCAGTTCTTC CGTGACATGG ATGATGAGGA GTCCTGGATC
    AAAGAGAAGA AACTGTTGGT GAGCTCAGAG GACTATGGCA GAGACCTGAC TGGTGTGCAG
    AACCTGAGGA AGAAACATAA GCGCTTGGAA GCAGAATTAG CTGCCCATGA ACCTGCTATC
    CAGGGTGTTC TAGACACTGG CAAGAAGCTT TCAGATGATA ACACAATTGG GAAGGAGGAG
    ATACAGCAAA GACTGGCTCA GTTTGTGGAC CACTGGAAAG AGTTAAAACA GCTGGCAGCT
    GCTCGGGGGC AGCGTCTGGA GGAGTCCCTG GAGTACCAAC AGTTTGTAGC AAATGTTGAG
    GAAGAGGAGG CATGGATCAA CGAGAAAATG ACATTGGTAG CCAGTGAGGA TTATGGAGAC
    ACACTTGCTG CTATCCAGGG CTTGCTGAAG AAGCATGAAG CATTCGAGAC TGACTTTACT
    GTCCACAAAG ACAGAGTGAA TGATGTTTGC GCTAATGGAG AGGATCTCAT TAAAAAGAAT
    AATCACCATG TGGAGAACAT TACTGCTAAG ATGAAGGGCC TCAAGGGCAA GGTATCAGAT
    CTGGAGAAAG CCGCAGCTCA GAGGAAAGCC AAACTGGATG AGAACTCTGC CTTCCTCCAG
    TTCAACTGGA AAGCAGATGT GGTGGAGTCA TGGATAGGTG AGAAGGAAAA CAGTCTGAAG
    ACAGATGATT ATGGCCGTGA CCTCTCCTCT GTGCAAACAC TACTCACCAA ACAGGAAACC
    TTTGATGCTG GACTTCAGGC TTTCCAGCAG GAGGGAATTG CAAACATCAC TGCTCTGAAA
    GACCAGCTAC TGGCAGCCAA ACATATCCAA TCAAAGGCCA TTGAGGTTCG TCACGCTTCC
    TTGATGAAAC GCTGGAATCA GCTGCTTGCT AATTCTGCAG CCAGGAAAAA GAAACTCTTG
    GAGGCTCAGG AGCACTTCAG AAAGGTTGAA GATCTCTTCT TGACTTTTGC CAAGAAGGCC
    TCTGCCTTCA ACAGTTGGTT TGAGAATGCT GAGGAGGACC TGACGGATCC CGTGCGCTGC
    AATTCCCTGG AAGAAATCAA AGCACTGCGA GAAGCCCACG ATGCGTTCCG TTCCTCACTT
    AGCTCTGCCC AAGCTGACTT CAACCAGCTG GCAGAGCTTG ATCGGCAGAT CAAGAGCTTC
    CGTGTAGCCT CCAACCCGTA CACTTGGTTT ACTATGGAGG CTCTTGAAGA AACTTGGAGG
    AATCTGCAGA AAATTATCAA GGAGCGTGAA TTGGAGTTGC AGAAGGAGCA GCGGAGGCAG
    GAAGAAAATG ACAAGTTGCG CCAGGAGTTT GCTCAGCATG CTAATGCCTT CCACCAGTGG
    ATTCAGGAGA CAAGGTCCTG CATGGTGGAG GAGTCGGGAA CACTGGAATC CCAGCTGGAA
    GCTACTAAAC GCAAGCACCA GGAGATCCGG GCTATGAGGA GCCAGCTGAA GAAGATTGAG
    GACCTTGGAG CAGCCATGGA AGAGGCACTT ATCTTGGACA ACAAATACAC AGAGCACAGC
    ACCGTGGGGC TGGCGCAGCA GTGGGACCAG CTTGACCAGC TGGGAATGAG AATGCAGCAC
    AATCTGGAAC AGCAGATTCA AGCTCGAAAC ACAACTGGAG TCACAGAGGA GGCCCTGAAG
    GAGTTCAGCA TGATGTTCAA GCACTTTGAC AAGGACAAAT CTGGACGTCT TAATCACCAG
    GAGTTTAAGT CTTGCTTGCG CTCCCTTGGC TATGACCTGC CCATGGTTGA GGAAGGAGAG
    CCTGACCCCG AATTTGAGTC TATTCTTGAC ACTGTTGATC CCAACAGGGA TGGCCACGTC
    TCGCTGCAGG AGTACATGGC GTTCATGATC AGCAGGGAAA CAGAGAACGT GAAATCCAGC
    GAGGAGATTG AGAGTGCTTT CCGTGCCCTC AGCTCGGAGG GGAAGCCTTA CGTGACCAAG
    GAGGAGCTCT ACCAGAACCT GACCCGGGAG CAGGCCGACT ACTGCATTTC TCACATGAAG
    CCCTACATGG ATGGCAAGGG CAGAGAACTT CCTTCTGCCT ACGACTACAT AGAATTTACA
    CGTTCACTCT TTGTGAATTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015134774.1
    CDS18..7538
    Translation

    Target ORF information:

    RefSeq Version XM_015279288.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015279288.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
    TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG
    TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC
    TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT
    GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT
    GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG
    ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAT CTTTCACGCC TTACAGGACT
    GCACAGCGCC CAATAGTAAC AGCCACCCAA GTTGGGTCCC ACATACGAAC TCGTCTACAG
    GAGCTGCACC GACTATGGGA GTTACTGCTG GAAAAAATGA GAGAGAAGGG AGTGAAGCTG
    TTGCAAGCAC AGAAGCTGGT GCAATTCTTA CGGGAATGTG AAGATGTCAT GGACTGGATC
    AATGACAAGG AAGCAATAGT GACCTCAGAA GAGCTTGGAC AGGACTTAGA GCATGTTGAG
    GTTTTGCAGA AGAAATTTGA AGAGTTCCAA ACAGATCTGG CAGCTCATGA GGAGAGAGTG
    AATGAAGTGA ACCAGTTCGC TGGCAAACTT ATCCAGGAAC AGCACCCTGA GGAGGAACTT
    ATAAAGTCCA AACAGGATGA AGTAAATGCA AGCTGGCAGC GTCTTAAGGG GCTTGCCCAA
    CAGAGGCAAG GAAAACTCTT TGGGGCAGCT GAAGTTCAGC GCTTCAACAG GGATGTGGAT
    GAAACTATCA GCTGGATTAA GGAGAAAGGG CAGTTGATGG CCTCAGATGA CTTTGGCAGA
    GACTTGGCCA GCGTGCAAGC TTTACTACGT AAGCATGAAG GCCTGGAAAG AGATCTTGCA
    GCTTTGGAGG ATAAGGTTAA GGCCCTCTGT GCAGAAGCTG ACCGTTTGCA GCAGTCTCAC
    CCTATAAATG CTTCTCAAAT TCAAGTGAAA CGGGAGGAAC TGATTGCCAA CTGGGAGCAG
    ATCCGAACTC TGGCAGCAGA GAGGCATGCT CGCCTTAATG ACTCCTACAG GTTGCAGCGC
    TTTCTTGCAG ATTTCCGGGA CCTCACTAGC TGGGTAACTG AGATGAAGGC TCTGATAAAT
    GCTGATGAGC TTGCCAATGA TGTGGCTGGA GCAGAAGCCC TTCTAGATAG ACATCAGGAA
    CATAAGGGAG AAATTGATGC TCATGAGGAT AGCTTCAGAT CTGCTGATGA GTCTGGGCAG
    GCTTTGCTTG CAGCTGGGCA CTATGCTTCT GATGAAGTTA AAGAAAAGCT GACTATCCTC
    TCAGATGAAA GATCAGCCTT GCTGGAACTA TGGGAGCTTC GCAGGCAACA GTATGAGCAG
    TGCATGGATC TGCAGCTTTT CTACAGAGAT ACTGAACAAG TTGACAACTG GATGAGCAAA
    CAAGAAGCTT TTCTGCTGAA CGAAGACCTT GGTGATTCTC TGGATAGCGT GGAGGCTCTT
    CTAAAGAAGC ATGAAGATTT TGAGAAATCC CTAAGTGCTC AAGAGGAGAA GATCACAGCA
    CTGGATGAGT TTGCTACTAA ACTGATTCAA AATAACCATT ATGCCATGGA TGACGTTGCT
    ACGCGCAGAG ATGCTCTTCT GAGTCGCCGA AATGCTCTTC ATGAAAGAGC CATGAAGCGC
    CGTGCCCAAC TGGCAGACTC TTTCCATCTC CAGCAGTTTT TCAGGGACTC TGATGAACTG
    AAGAGTTGGG TTAATGAAAA AATGAAAACT GCTACCGATG AGGCTTACAA GGATCCATCC
    AACTTGCAAG GTAAAGTTCA GAAGCATCAG GCTTTTGAAG CAGAGCTTTC TGCTAATCAG
    AGTCGTATTG ATGCACTGGA GAAAGCTGGC CAGAAGTTGA TTGATGTCAA TCACTATGCA
    TCTGATGAGG TGGCAGCTCG CATGAATGAA GTCATCAGCT TGTGGAAGAA ACTTCTGGAA
    GCCACCGAGC TCAAAGGTAT AAAGCTGCGT GAAGCCAATC AACAGCAGCA ATTTAATCGC
    AATGTAGAAG ACATTGAGTT GTGGCTGTAT GAAGTGGAGG GTCACTTGGC TTCTGATGAT
    TATGGAAAAG ATCTTACCAG CGTTCAGAAT CTTCAGAAGA AACATGCCCT GCTAGAGGCA
    GATGTTGCTG CCCATCAGGA TCGGATAGAT GGCATTACCA TCCAGGCACG CCAGTTCCAA
    GATGCTGGGC ACTTTGATGC TGACAATATC AAGAAGAAAC AAGAAGCTTT AGTAGCTCGT
    TATGAAGCTC TGAAGGATCC TATGGTGGCT CGCAAGCAGA AACTTGCAGA TTCTCTTCGC
    CTGCAGCAGC TTTTCCGTGA CATCGAGGAT GAAGAGACCT GGATCAGGGA AAAGGAACCT
    ATTGCAGCCT CAACAAACCG AGGCAAGGAC TTAATTGGTG TCCAGAATCT GCTAAAGAAG
    CACCAGGCTT TGCAGGCAGA AATTGCAGGC CATGAGCCTC GCATTAAAGC AGTCACACAA
    AAAGGAAATG CGATGGTGGA AGAGGGACAT TTTGCTGCTG AAGATGTGAA GATCAAATTG
    AATGAACTAA ACCAAAAATG GGACTCTCTG AAAGCCAAGG CATCTCAACG GCGACAGGAT
    CTAGAGGATT CTCTGCAAGC TCAGCAGTAT TTTGCTGATG CTAATGAGGC AGAATCATGG
    ATGAGGGAAA AGGAGCCCAT TGTAGGCAGC ACGGATTATG GAAAGGATGA AGACTCTGCT
    GAGGCTCTCC TGAAGAAGCA TGAAGCTTTG ATGTCTGATC TTTCTGCTTA CGGCAGTAGC
    ATACAGGCAT TAAGGGAACA GGCCCAGTCC TGCAGGCAAC AAGTTGCTCC CACTGATGAT
    GAAACTGGAA AAGAGCTTGT GCTAGCACTC TATGATTACC AAGAGAAGAG TCCTCGGGAG
    GTGACTATGA AGAAAGGAGA TATTCTAACC CTGCTCAACA GCACCAACAA GGACTGGTGG
    AAGGTTGAAG TTAACGATCG TCAGGGCTTT GTACCAGCTG CCTATGTGAA AAAACTAGAT
    CCTGCCCAGT CTGCATCCCG AGAGAATCTT CTAGAAGAGC AAGGCAGCAT AGCATTGCGA
    CAGGAGCAAA TTGACAACCA GACTCTCATT ACTAAGGAAG TCGGCAGTGT ATCTCTGCGT
    ATGAAACAGG TCGAAGAACT GTATCATTCC CTTCTTGAGC TGGGAGAAAA ACGTAAAGGC
    ATGCTAGAAA AAAGCTGCAA GAAGTTTATG CTTTTCCGTG AGGCTAATGA GCTTCAACAG
    TGGATCAATG AGAAGGAAGC TGCGCTCACT AATGAGGAAG TGGGTGCTGA TTTGGAGCAG
    GTGGAGGTGC TACAGAAGAA GTTTGATGAT TTTCAGAAGG ATTTAAAAGC TAATGAGTCA
    CGACTGAAGG ACATAAACAA GGTTGCAAAT GACCTGGAGT CTGAAGGGCT GATGGCAGAG
    GAAGTGCAAG CAGTAGAGCA CCAGGAAGTC TATGGAATGA TGCCCAGAGA TGAAACTGAT
    TCTAAGACAG CCTCTCCTTG GAAGTCTGCA CGTATGATGG TACACACAGT GGCAACGTTT
    AACTCAATCA AGGAACTGAA TGAACGCTGG AGATCCCTGC AGCAGTTGGC AGAGGAGCGA
    AGCCAGCTGT TGGGCAGCGC TCATGAAGTC CAGAGGTTCC ACAGAGATGC TGATGAAACA
    AAAGAATGGA TAGAAGAGAA GAATCAAGCA TTAAATACCG ACAACTATGG ACATGACTTG
    GCCAGTGTGC AGGCTCTGCA GCGCAAGCAT GAAGGCTTTG AGAGAGACTT GGCAGCTCTT
    GGAGACAAGG TGAATTCTCT TGGTGAAACT GCCCAGCGTC TGATCCAGTC ACATCCAGAA
    TCAGCTGAAG ATCTTCAAGA AAAATGTACT GAGTTGAACC AAGCCTGGAG TAGTCTGGGA
    AAACGTGCTG ACCAACGCAA AGAGAAGCTT GGAGATTCTC ATGACCTGCA GCGTTTCCTC
    AGTGATTTTA GAGACCTTAT GTCTTGGATC AATGGAATCC GGGGTCTGGT CTCCTCAGAT
    GAACTTGCAA AAGATGTGAC TGGAGCTGAA GCTCTGTTGG AAAGGCATCA GGAGCACCGT
    ACTGAAATAG ATGCACGGGC TGGCACTTTT CAGGCATTTG AACAGTTTGG ACAGCAGCTT
    CTGGCTCGTG GACACTATGC CAGCCCAGAG ATTAAGGAGA AACTGGATAT TCTAGATCAA
    GAACGGACAG ACCTAGAGAA GGCCTGGGTC CAGCGCAGAA TGATGCTAGA CCAGTGCTTA
    GAACTACAGC TGTTTCATCG GGATTGTGAA CAAGCTGAAA ACTGGATGGC TGCCCGAGAG
    GCGTTCCTAA ATACAGAAGA CAAAGGAGAC TCCTTAGACA GCGTGGAGGC ACTCATCAAG
    AAGCATGAAG ATTTCGATAA AGCAATCAAT GTCCAGGAAG AGAAAATTGC TGTCTTGCAG
    TCTTTTGCTG ACCAACTGAT TGCTGCTGAT CATTATGCAA AAGGAGTCAT TGCTAACAGA
    CGCAATGAGG TTCTGGACAG GTGGCGTCGT CTGAAGGCTC AGATGATTGA GAAGAGATCT
    AAGCTGGGAG AATCTCAGAC CCTCCAACAG TTCAGTCGTG ATGTTGATGA AATAGAAGCT
    TGGATCAGTG AAAAGCTTCA AACTGCAAGT GATGAGTCAT ATAAGGATCC CACAAACATC
    CAGCTTTCCA AACTGCTGAG CAAGCACCAG AAGCACCAAG CCTTTGAAGC TGAGCTCCAC
    GCCAACGCAG ATCGGATTCG TGGAGTCATT GAAATGGGGA ACTCTCTTAT TGAAAGAGGA
    GCGTGTGCTG GCAGCGAGGA TGCTGTGAAG GCTCGGCTGG CTGCCCTGGC TGACCAATGG
    GAGTTCCTGG TCCAGAAGTC ATCAGAGAAG AGTCAGAAAC TGAAAGAAGC AAATAAACAG
    CAGAATTTCA ATACCGGCAT CAAGGACTTT GATTTCTGGC TTTCAGAGGT GGAAGCTTTG
    TTGGCATCTG AAGACTATGG GAAGGACTTG GCATCAGTGA ACAACCTTCT GAAGAAGCAC
    CAATTACTGG AAGCTGATAT ATCTGCTCAT GAGGATCGGC TGAAGGACCT GAACAGCCAG
    GCTGACAGTT TGATGACCAG CAGTGCTTTC GATACCTCCC AAGTAAAGGA TAAACGTGAA
    ACTATAAATG GGCGCTTCCA GAGAATCAAG AGCATGGCAG CTGCCCGCCG TGCGAAGCTC
    AACGAGTCGC ACCGCTTGCA TCAGTTCTTC CGTGACATGG ATGATGAGGA GTCCTGGATC
    AAAGAGAAGA AACTGTTGGT GAGCTCAGAG GACTATGGCA GAGACCTGAC TGGTGTGCAG
    AACCTGAGGA AGAAACATAA GCGCTTGGAA GCAGAATTAG CTGCCCATGA ACCTGCTATC
    CAGGGTGTTC TAGACACTGG CAAGAAGCTT TCAGATGATA ACACAATTGG GAAGGAGGAG
    ATACAGCAAA GACTGGCTCA GTTTGTGGAC CACTGGAAAG AGTTAAAACA GCTGGCAGCT
    GCTCGGGGGC AGCGTCTGGA GGAGTCCCTG GAGTACCAAC AGTTTGTAGC AAATGTTGAG
    GAAGAGGAGG CATGGATCAA CGAGAAAATG ACATTGGTAG CCAGTGAGGA TTATGGAGAC
    ACACTTGCTG CTATCCAGGG CTTGCTGAAG AAGCATGAAG CATTCGAGAC TGACTTTACT
    GTCCACAAAG ACAGAGTGAA TGATGTTTGC GCTAATGGAG AGGATCTCAT TAAAAAGAAT
    AATCACCATG TGGAGAACAT TACTGCTAAG ATGAAGGGCC TCAAGGGCAA GGTATCAGAT
    CTGGAGAAAG CCGCAGCTCA GAGGAAAGCC AAACTGGATG AGAACTCTGC CTTCCTCCAG
    TTCAACTGGA AAGCAGATGT GGTGGAGTCA TGGATAGGTG AGAAGGAAAA CAGTCTGAAG
    ACAGATGATT ATGGCCGTGA CCTCTCCTCT GTGCAAACAC TACTCACCAA ACAGGAAACC
    TTTGATGCTG GACTTCAGGC TTTCCAGCAG GAGGGAATTG CAAACATCAC TGCTCTGAAA
    GACCAGCTAC TGGCAGCCAA ACATATCCAA TCAAAGGCCA TTGAGGTTCG TCACGCTTCC
    TTGATGAAAC GCTGGAATCA GCTGCTTGCT AATTCTGCAG CCAGGAAAAA GAAACTCTTG
    GAGGCTCAGG AGCACTTCAG AAAGGTTGAA GATCTCTTCT TGACTTTTGC CAAGAAGGCC
    TCTGCCTTCA ACAGTTGGTT TGAGAATGCT GAGGAGGACC TGACGGATCC CGTGCGCTGC
    AATTCCCTGG AAGAAATCAA AGCACTGCGA GAAGCCCACG ATGCGTTCCG TTCCTCACTT
    AGCTCTGCCC AAGCTGACTT CAACCAGCTG GCAGAGCTTG ATCGGCAGAT CAAGAGCTTC
    CGTGTAGCCT CCAACCCGTA CACTTGGTTT ACTATGGAGG CTCTTGAAGA AACTTGGAGG
    AATCTGCAGA AAATTATCAA GGAGCGTGAA TTGGAGTTGC AGAAGGAGCA GCGGAGGCAG
    GAAGAAAATG ACAAGTTGCG CCAGGAGTTT GCTCAGCATG CTAATGCCTT CCACCAGTGG
    ATTCAGGAGA CAAGGTCCTG CATGGTGGAG GAGTCGGGAA CACTGGAATC CCAGCTGGAA
    GCTACTAAAC GCAAGCACCA GGAGATCCGG GCTATGAGGA GCCAGCTGAA GAAGATTGAG
    GACCTTGGAG CAGCCATGGA AGAGGCACTT ATCTTGGACA ACAAATACAC AGAGCACAGC
    ACCGTGGGGC TGGCGCAGCA GTGGGACCAG CTTGACCAGC TGGGAATGAG AATGCAGCAC
    AATCTGGAAC AGCAGATTCA AGCTCGAAAC ACAACTGGAG TCACAGAGGA GGCCCTGAAG
    GAGTTCAGCA TGATGTTCAA GCACTTTGAC AAGGACAAAT CTGGACGTCT TAATCACCAG
    GAGTTTAAGT CTTGCTTGCG CTCCCTTGGC TATGACCTGC CCATGGTTGA GGAAGGAGAG
    CCTGACCCCG AATTTGAGTC TATTCTTGAC ACTGTTGATC CCAACAGGGA TGGCCACGTC
    TCGCTGCAGG AGTACATGGC GTTCATGATC AGCAGGGAAA CAGAGAACGT GAAATCCAGC
    GAGGAGATTG AGAGTGCTTT CCGTGCCCTC AGCTCGGAGG GGAAGCCTTA CGTGACCAAG
    GAGGAGCTCT ACCAGAACCT GACCCGGGAG CAGGCCGACT ACTGCATTTC TCACATGAAG
    CCCTACATGG ATGGCAAGGG CAGAGAACTT CCTTCTGCCT ACGACTACAT AGAATTTACA
    CGTTCACTCT TTGTGAATTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa43315
    Clone ID Related Accession (Same CDS sequence) XM_015279289.1 , XM_015279289.2
    Accession Version XM_015279289.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7497bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGGATCCAA GTGGGGTAAA AGTGTTGGAA ACAGCAGAAG ATATCCAAGA ACGGCGTCAG 
    CAGGTTTTGG ACCGTTACCA CAGGTTCAAG GAGCTGTCTT CTCTGAGGCG CCAAAAACTC
    GAAGATTCCT ATCGGTTCCA GTTCTTCCAG CGCGATGCAG ATGAGCTGGA AAAATGGATC
    CAAGAGAAAC TCCAGATAGC ATCTGATGAA AATTACAAAG ACCCAAGCAA TTTACAGGGG
    AAGCTGCAGA AGCACCAGGC CTTTGAAGCT GAGGTGCAGG CCAATTCAGG GGCTATCGTT
    AAGCTGGATG AGACTGGAAA TCAGATGATC AATGAAGGCC ATTTTGCATC TGAAACCATA
    AGAACTCGTC TACAGGAGCT GCACCGACTA TGGGAGTTAC TGCTGGAAAA AATGAGAGAG
    AAGGGAGTGA AGCTGTTGCA AGCACAGAAG CTGGTGCAAT TCTTACGGGA ATGTGAAGAT
    GTCATGGACT GGATCAATGA CAAGGAAGCA ATAGTGACCT CAGAAGAGCT TGGACAGGAC
    TTAGAGCATG TTGAGGTTTT GCAGAAGAAA TTTGAAGAGT TCCAAACAGA TCTGGCAGCT
    CATGAGGAGA GAGTGAATGA AGTGAACCAG TTCGCTGGCA AACTTATCCA GGAACAGCAC
    CCTGAGGAGG AACTTATAAA GTCCAAACAG GATGAAGTAA ATGCAAGCTG GCAGCGTCTT
    AAGGGGCTTG CCCAACAGAG GCAAGGAAAA CTCTTTGGGG CAGCTGAAGT TCAGCGCTTC
    AACAGGGATG TGGATGAAAC TATCAGCTGG ATTAAGGAGA AAGGGCAGTT GATGGCCTCA
    GATGACTTTG GCAGAGACTT GGCCAGCGTG CAAGCTTTAC TACGTAAGCA TGAAGGCCTG
    GAAAGAGATC TTGCAGCTTT GGAGGATAAG GTTAAGGCCC TCTGTGCAGA AGCTGACCGT
    TTGCAGCAGT CTCACCCTAT AAATGCTTCT CAAATTCAAG TGAAACGGGA GGAACTGATT
    GCCAACTGGG AGCAGATCCG AACTCTGGCA GCAGAGAGGC ATGCTCGCCT TAATGACTCC
    TACAGGTTGC AGCGCTTTCT TGCAGATTTC CGGGACCTCA CTAGCTGGGT AACTGAGATG
    AAGGCTCTGA TAAATGCTGA TGAGCTTGCC AATGATGTGG CTGGAGCAGA AGCCCTTCTA
    GATAGACATC AGGAACATAA GGGAGAAATT GATGCTCATG AGGATAGCTT CAGATCTGCT
    GATGAGTCTG GGCAGGCTTT GCTTGCAGCT GGGCACTATG CTTCTGATGA AGTTAAAGAA
    AAGCTGACTA TCCTCTCAGA TGAAAGATCA GCCTTGCTGG AACTATGGGA GCTTCGCAGG
    CAACAGTATG AGCAGTGCAT GGATCTGCAG CTTTTCTACA GAGATACTGA ACAAGTTGAC
    AACTGGATGA GCAAACAAGA AGCTTTTCTG CTGAACGAAG ACCTTGGTGA TTCTCTGGAT
    AGCGTGGAGG CTCTTCTAAA GAAGCATGAA GATTTTGAGA AATCCCTAAG TGCTCAAGAG
    GAGAAGATCA CAGCACTGGA TGAGTTTGCT ACTAAACTGA TTCAAAATAA CCATTATGCC
    ATGGATGACG TTGCTACGCG CAGAGATGCT CTTCTGAGTC GCCGAAATGC TCTTCATGAA
    AGAGCCATGA AGCGCCGTGC CCAACTGGCA GACTCTTTCC ATCTCCAGCA GTTTTTCAGG
    GACTCTGATG AACTGAAGAG TTGGGTTAAT GAAAAAATGA AAACTGCTAC CGATGAGGCT
    TACAAGGATC CATCCAACTT GCAAGGTAAA GTTCAGAAGC ATCAGGCTTT TGAAGCAGAG
    CTTTCTGCTA ATCAGAGTCG TATTGATGCA CTGGAGAAAG CTGGCCAGAA GTTGATTGAT
    GTCAATCACT ATGCATCTGA TGAGGTGGCA GCTCGCATGA ATGAAGTCAT CAGCTTGTGG
    AAGAAACTTC TGGAAGCCAC CGAGCTCAAA GGTATAAAGC TGCGTGAAGC CAATCAACAG
    CAGCAATTTA ATCGCAATGT AGAAGACATT GAGTTGTGGC TGTATGAAGT GGAGGGTCAC
    TTGGCTTCTG ATGATTATGG AAAAGATCTT ACCAGCGTTC AGAATCTTCA GAAGAAACAT
    GCCCTGCTAG AGGCAGATGT TGCTGCCCAT CAGGATCGGA TAGATGGCAT TACCATCCAG
    GCACGCCAGT TCCAAGATGC TGGGCACTTT GATGCTGACA ATATCAAGAA GAAACAAGAA
    GCTTTAGTAG CTCGTTATGA AGCTCTGAAG GATCCTATGG TGGCTCGCAA GCAGAAACTT
    GCAGATTCTC TTCGCCTGCA GCAGCTTTTC CGTGACATCG AGGATGAAGA GACCTGGATC
    AGGGAAAAGG AACCTATTGC AGCCTCAACA AACCGAGGCA AGGACTTAAT TGGTGTCCAG
    AATCTGCTAA AGAAGCACCA GGCTTTGCAG GCAGAAATTG CAGGCCATGA GCCTCGCATT
    AAAGCAGTCA CACAAAAAGG AAATGCGATG GTGGAAGAGG GACATTTTGC TGCTGAAGAT
    GTGAAGATCA AATTGAATGA ACTAAACCAA AAATGGGACT CTCTGAAAGC CAAGGCATCT
    CAACGGCGAC AGGATCTAGA GGATTCTCTG CAAGCTCAGC AGTATTTTGC TGATGCTAAT
    GAGGCAGAAT CATGGATGAG GGAAAAGGAG CCCATTGTAG GCAGCACGGA TTATGGAAAG
    GATGAAGACT CTGCTGAGGC TCTCCTGAAG AAGCATGAAG CTTTGATGTC TGATCTTTCT
    GCTTACGGCA GTAGCATACA GGCATTAAGG GAACAGGCCC AGTCCTGCAG GCAACAAGTT
    GCTCCCACTG ATGATGAAAC TGGAAAAGAG CTTGTGCTAG CACTCTATGA TTACCAAGAG
    AAGAGTCCTC GGGAGGTGAC TATGAAGAAA GGAGATATTC TAACCCTGCT CAACAGCACC
    AACAAGGACT GGTGGAAGGT TGAAGTTAAC GATCGTCAGG GCTTTGTACC AGCTGCCTAT
    GTGAAAAAAC TAGATCCTGC CCAGTCTGCA TCCCGAGAGA ATCTTCTAGA AGAGCAAGGC
    AGCATAGCAT TGCGACAGGA GCAAATTGAC AACCAGACTC TCATTACTAA GGAAGTCGGC
    AGTGTATCTC TGCGTATGAA ACAGGTCGAA GAACTGTATC ATTCCCTTCT TGAGCTGGGA
    GAAAAACGTA AAGGCATGCT AGAAAAAAGC TGCAAGAAGT TTATGCTTTT CCGTGAGGCT
    AATGAGCTTC AACAGTGGAT CAATGAGAAG GAAGCTGCGC TCACTAATGA GGAAGTGGGT
    GCTGATTTGG AGCAGGTGGA GGTGCTACAG AAGAAGTTTG ATGATTTTCA GAAGGATTTA
    AAAGCTAATG AGTCACGACT GAAGGACATA AACAAGGTTG CAAATGACCT GGAGTCTGAA
    GGGCTGATGG CAGAGGAAGT GCAAGCAGTA GAGCACCAGG AAGTCTATGG AATGATGCCC
    AGAGATGAAA CTGATTCTAA GACAGCCTCT CCTTGGAAGT CTGCACGTAT GATGGTACAC
    ACAGTGGCAA CGTTTAACTC AATCAAGGAA CTGAATGAAC GCTGGAGATC CCTGCAGCAG
    TTGGCAGAGG AGCGAAGCCA GCTGTTGGGC AGCGCTCATG AAGTCCAGAG GTTCCACAGA
    GATGCTGATG AAACAAAAGA ATGGATAGAA GAGAAGAATC AAGCATTAAA TACCGACAAC
    TATGGACATG ACTTGGCCAG TGTGCAGGCT CTGCAGCGCA AGCATGAAGG CTTTGAGAGA
    GACTTGGCAG CTCTTGGAGA CAAGGTGAAT TCTCTTGGTG AAACTGCCCA GCGTCTGATC
    CAGTCACATC CAGAATCAGC TGAAGATCTT CAAGAAAAAT GTACTGAGTT GAACCAAGCC
    TGGAGTAGTC TGGGAAAACG TGCTGACCAA CGCAAAGAGA AGCTTGGAGA TTCTCATGAC
    CTGCAGCGTT TCCTCAGTGA TTTTAGAGAC CTTATGTCTT GGATCAATGG AATCCGGGGT
    CTGGTCTCCT CAGATGAACT TGCAAAAGAT GTGACTGGAG CTGAAGCTCT GTTGGAAAGG
    CATCAGGAGC ACCGTACTGA AATAGATGCA CGGGCTGGCA CTTTTCAGGC ATTTGAACAG
    TTTGGACAGC AGCTTCTGGC TCGTGGACAC TATGCCAGCC CAGAGATTAA GGAGAAACTG
    GATATTCTAG ATCAAGAACG GACAGACCTA GAGAAGGCCT GGGTCCAGCG CAGAATGATG
    CTAGACCAGT GCTTAGAACT ACAGCTGTTT CATCGGGATT GTGAACAAGC TGAAAACTGG
    ATGGCTGCCC GAGAGGCGTT CCTAAATACA GAAGACAAAG GAGACTCCTT AGACAGCGTG
    GAGGCACTCA TCAAGAAGCA TGAAGATTTC GATAAAGCAA TCAATGTCCA GGAAGAGAAA
    ATTGCTGTCT TGCAGTCTTT TGCTGACCAA CTGATTGCTG CTGATCATTA TGCAAAAGGA
    GTCATTGCTA ACAGACGCAA TGAGGTTCTG GACAGGTGGC GTCGTCTGAA GGCTCAGATG
    ATTGAGAAGA GATCTAAGCT GGGAGAATCT CAGACCCTCC AACAGTTCAG TCGTGATGTT
    GATGAAATAG AAGCTTGGAT CAGTGAAAAG CTTCAAACTG CAAGTGATGA GTCATATAAG
    GATCCCACAA ACATCCAGCT TTCCAAACTG CTGAGCAAGC ACCAGAAGCA CCAAGCCTTT
    GAAGCTGAGC TCCACGCCAA CGCAGATCGG ATTCGTGGAG TCATTGAAAT GGGGAACTCT
    CTTATTGAAA GAGGAGCGTG TGCTGGCAGC GAGGATGCTG TGAAGGCTCG GCTGGCTGCC
    CTGGCTGACC AATGGGAGTT CCTGGTCCAG AAGTCATCAG AGAAGAGTCA GAAACTGAAA
    GAAGCAAATA AACAGCAGAA TTTCAATACC GGCATCAAGG ACTTTGATTT CTGGCTTTCA
    GAGGTGGAAG CTTTGTTGGC ATCTGAAGAC TATGGGAAGG ACTTGGCATC AGTGAACAAC
    CTTCTGAAGA AGCACCAATT ACTGGAAGCT GATATATCTG CTCATGAGGA TCGGCTGAAG
    GACCTGAACA GCCAGGCTGA CAGTTTGATG ACCAGCAGTG CTTTCGATAC CTCCCAAGTA
    AAGGATAAAC GTGAAACTAT AAATGGGCGC TTCCAGAGAA TCAAGAGCAT GGCAGCTGCC
    CGCCGTGCGA AGCTCAACGA GTCGCACCGC TTGCATCAGT TCTTCCGTGA CATGGATGAT
    GAGGAGTCCT GGATCAAAGA GAAGAAACTG TTGGTGAGCT CAGAGGACTA TGGCAGAGAC
    CTGACTGGTG TGCAGAACCT GAGGAAGAAA CATAAGCGCT TGGAAGCAGA ATTAGCTGCC
    CATGAACCTG CTATCCAGGG TGTTCTAGAC ACTGGCAAGA AGCTTTCAGA TGATAACACA
    ATTGGGAAGG AGGAGATACA GCAAAGACTG GCTCAGTTTG TGGACCACTG GAAAGAGTTA
    AAACAGCTGG CAGCTGCTCG GGGGCAGCGT CTGGAGGAGT CCCTGGAGTA CCAACAGTTT
    GTAGCAAATG TTGAGGAAGA GGAGGCATGG ATCAACGAGA AAATGACATT GGTAGCCAGT
    GAGGATTATG GAGACACACT TGCTGCTATC CAGGGCTTGC TGAAGAAGCA TGAAGCATTC
    GAGACTGACT TTACTGTCCA CAAAGACAGA GTGAATGATG TTTGCGCTAA TGGAGAGGAT
    CTCATTAAAA AGAATAATCA CCATGTGGAG AACATTACTG CTAAGATGAA GGGCCTCAAG
    GGCAAGGTAT CAGATCTGGA GAAAGCCGCA GCTCAGAGGA AAGCCAAACT GGATGAGAAC
    TCTGCCTTCC TCCAGTTCAA CTGGAAAGCA GATGTGGTGG AGTCATGGAT AGGTGAGAAG
    GAAAACAGTC TGAAGACAGA TGATTATGGC CGTGACCTCT CCTCTGTGCA AACACTACTC
    ACCAAACAGG AAACCTTTGA TGCTGGACTT CAGGCTTTCC AGCAGGAGGG AATTGCAAAC
    ATCACTGCTC TGAAAGACCA GCTACTGGCA GCCAAACATA TCCAATCAAA GGCCATTGAG
    GTTCGTCACG CTTCCTTGAT GAAACGCTGG AATCAGCTGC TTGCTAATTC TGCAGCCAGG
    AAAAAGAAAC TCTTGGAGGC TCAGGAGCAC TTCAGAAAGG TTGAAGATCT CTTCTTGACT
    TTTGCCAAGA AGGCCTCTGC CTTCAACAGT TGGTTTGAGA ATGCTGAGGA GGACCTGACG
    GATCCCGTGC GCTGCAATTC CCTGGAAGAA ATCAAAGCAC TGCGAGAAGC CCACGATGCG
    TTCCGTTCCT CACTTAGCTC TGCCCAAGCT GACTTCAACC AGCTGGCAGA GCTTGATCGG
    CAGATCAAGA GCTTCCGTGT AGCCTCCAAC CCGTACACTT GGTTTACTAT GGAGGCTCTT
    GAAGAAACTT GGAGGAATCT GCAGAAAATT ATCAAGGAGC GTGAATTGGA GTTGCAGAAG
    GAGCAGCGGA GGCAGGAAGA AAATGACAAG TTGCGCCAGG AGTTTGCTCA GCATGCTAAT
    GCCTTCCACC AGTGGATTCA GGAGACAAGG ACTTACCTGC TAGATGGCAT AGCCTATCGT
    CGTGTCATTC GTGTCTATCA GTATGAAGTT GGGGATGATC TATCTGGAAG GTCCTGCATG
    GTGGAGGAGT CGGGAACACT GGAATCCCAG CTGGAAGCTA CTAAACGCAA GCACCAGGAG
    ATCCGGGCTA TGAGGAGCCA GCTGAAGAAG ATTGAGGACC TTGGAGCAGC CATGGAAGAG
    GCACTTATCT TGGACAACAA ATACACAGAG CACAGCACCG TGGGGCTGGC GCAGCAGTGG
    GACCAGCTTG ACCAGCTGGG AATGAGAATG CAGCACAATC TGGAACAGCA GATTCAAGCT
    CGAAACACAA CTGGAGTCAC AGAGGAGGCC CTGAAGGAGT TCAGCATGAT GTTCAAGCAC
    TTTGACAAGG ACAAATCTGG ACGTCTTAAT CACCAGGAGT TTAAGTCTTG CTTGCGCTCC
    CTTGGCTATG ACCTGCCCAT GGTTGAGGAA GGAGAGCCTG ACCCCGAATT TGAGTCTATT
    CTTGACACTG TTGATCCCAA CAGGGATGGC CACGTCTCGC TGCAGGAGTA CATGGCGTTC
    ATGATCAGCA GGGAAACAGA GAACGTGAAA TCCAGCGAGG AGATTGAGAG TGCTTTCCGT
    GCCCTCAGCT CGGAGGGGAA GCCTTACGTG ACCAAGGAGG AGCTCTACCA GAACCTGACC
    CGGGAGCAGG CCGACTACTG CATTTCTCAC ATGAAGCCCT ACATGGATGG CAAGGGCAGA
    GAACTTCCTT CTGCCTACGA CTACATAGAA TTTACACGTT CACTCTTTGT GAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015134775.1
    CDS110..7606
    Translation

    Target ORF information:

    RefSeq Version XM_015279289.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015279289.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGGATCCAA GTGGGGTAAA AGTGTTGGAA ACAGCAGAAG ATATCCAAGA ACGGCGTCAG 
    CAGGTTTTGG ACCGTTACCA CAGGTTCAAG GAGCTGTCTT CTCTGAGGCG CCAAAAACTC
    GAAGATTCCT ATCGGTTCCA GTTCTTCCAG CGCGATGCAG ATGAGCTGGA AAAATGGATC
    CAAGAGAAAC TCCAGATAGC ATCTGATGAA AATTACAAAG ACCCAAGCAA TTTACAGGGG
    AAGCTGCAGA AGCACCAGGC CTTTGAAGCT GAGGTGCAGG CCAATTCAGG GGCTATCGTT
    AAGCTGGATG AGACTGGAAA TCAGATGATC AATGAAGGCC ATTTTGCATC TGAAACCATA
    AGAACTCGTC TACAGGAGCT GCACCGACTA TGGGAGTTAC TGCTGGAAAA AATGAGAGAG
    AAGGGAGTGA AGCTGTTGCA AGCACAGAAG CTGGTGCAAT TCTTACGGGA ATGTGAAGAT
    GTCATGGACT GGATCAATGA CAAGGAAGCA ATAGTGACCT CAGAAGAGCT TGGACAGGAC
    TTAGAGCATG TTGAGGTTTT GCAGAAGAAA TTTGAAGAGT TCCAAACAGA TCTGGCAGCT
    CATGAGGAGA GAGTGAATGA AGTGAACCAG TTCGCTGGCA AACTTATCCA GGAACAGCAC
    CCTGAGGAGG AACTTATAAA GTCCAAACAG GATGAAGTAA ATGCAAGCTG GCAGCGTCTT
    AAGGGGCTTG CCCAACAGAG GCAAGGAAAA CTCTTTGGGG CAGCTGAAGT TCAGCGCTTC
    AACAGGGATG TGGATGAAAC TATCAGCTGG ATTAAGGAGA AAGGGCAGTT GATGGCCTCA
    GATGACTTTG GCAGAGACTT GGCCAGCGTG CAAGCTTTAC TACGTAAGCA TGAAGGCCTG
    GAAAGAGATC TTGCAGCTTT GGAGGATAAG GTTAAGGCCC TCTGTGCAGA AGCTGACCGT
    TTGCAGCAGT CTCACCCTAT AAATGCTTCT CAAATTCAAG TGAAACGGGA GGAACTGATT
    GCCAACTGGG AGCAGATCCG AACTCTGGCA GCAGAGAGGC ATGCTCGCCT TAATGACTCC
    TACAGGTTGC AGCGCTTTCT TGCAGATTTC CGGGACCTCA CTAGCTGGGT AACTGAGATG
    AAGGCTCTGA TAAATGCTGA TGAGCTTGCC AATGATGTGG CTGGAGCAGA AGCCCTTCTA
    GATAGACATC AGGAACATAA GGGAGAAATT GATGCTCATG AGGATAGCTT CAGATCTGCT
    GATGAGTCTG GGCAGGCTTT GCTTGCAGCT GGGCACTATG CTTCTGATGA AGTTAAAGAA
    AAGCTGACTA TCCTCTCAGA TGAAAGATCA GCCTTGCTGG AACTATGGGA GCTTCGCAGG
    CAACAGTATG AGCAGTGCAT GGATCTGCAG CTTTTCTACA GAGATACTGA ACAAGTTGAC
    AACTGGATGA GCAAACAAGA AGCTTTTCTG CTGAACGAAG ACCTTGGTGA TTCTCTGGAT
    AGCGTGGAGG CTCTTCTAAA GAAGCATGAA GATTTTGAGA AATCCCTAAG TGCTCAAGAG
    GAGAAGATCA CAGCACTGGA TGAGTTTGCT ACTAAACTGA TTCAAAATAA CCATTATGCC
    ATGGATGACG TTGCTACGCG CAGAGATGCT CTTCTGAGTC GCCGAAATGC TCTTCATGAA
    AGAGCCATGA AGCGCCGTGC CCAACTGGCA GACTCTTTCC ATCTCCAGCA GTTTTTCAGG
    GACTCTGATG AACTGAAGAG TTGGGTTAAT GAAAAAATGA AAACTGCTAC CGATGAGGCT
    TACAAGGATC CATCCAACTT GCAAGGTAAA GTTCAGAAGC ATCAGGCTTT TGAAGCAGAG
    CTTTCTGCTA ATCAGAGTCG TATTGATGCA CTGGAGAAAG CTGGCCAGAA GTTGATTGAT
    GTCAATCACT ATGCATCTGA TGAGGTGGCA GCTCGCATGA ATGAAGTCAT CAGCTTGTGG
    AAGAAACTTC TGGAAGCCAC CGAGCTCAAA GGTATAAAGC TGCGTGAAGC CAATCAACAG
    CAGCAATTTA ATCGCAATGT AGAAGACATT GAGTTGTGGC TGTATGAAGT GGAGGGTCAC
    TTGGCTTCTG ATGATTATGG AAAAGATCTT ACCAGCGTTC AGAATCTTCA GAAGAAACAT
    GCCCTGCTAG AGGCAGATGT TGCTGCCCAT CAGGATCGGA TAGATGGCAT TACCATCCAG
    GCACGCCAGT TCCAAGATGC TGGGCACTTT GATGCTGACA ATATCAAGAA GAAACAAGAA
    GCTTTAGTAG CTCGTTATGA AGCTCTGAAG GATCCTATGG TGGCTCGCAA GCAGAAACTT
    GCAGATTCTC TTCGCCTGCA GCAGCTTTTC CGTGACATCG AGGATGAAGA GACCTGGATC
    AGGGAAAAGG AACCTATTGC AGCCTCAACA AACCGAGGCA AGGACTTAAT TGGTGTCCAG
    AATCTGCTAA AGAAGCACCA GGCTTTGCAG GCAGAAATTG CAGGCCATGA GCCTCGCATT
    AAAGCAGTCA CACAAAAAGG AAATGCGATG GTGGAAGAGG GACATTTTGC TGCTGAAGAT
    GTGAAGATCA AATTGAATGA ACTAAACCAA AAATGGGACT CTCTGAAAGC CAAGGCATCT
    CAACGGCGAC AGGATCTAGA GGATTCTCTG CAAGCTCAGC AGTATTTTGC TGATGCTAAT
    GAGGCAGAAT CATGGATGAG GGAAAAGGAG CCCATTGTAG GCAGCACGGA TTATGGAAAG
    GATGAAGACT CTGCTGAGGC TCTCCTGAAG AAGCATGAAG CTTTGATGTC TGATCTTTCT
    GCTTACGGCA GTAGCATACA GGCATTAAGG GAACAGGCCC AGTCCTGCAG GCAACAAGTT
    GCTCCCACTG ATGATGAAAC TGGAAAAGAG CTTGTGCTAG CACTCTATGA TTACCAAGAG
    AAGAGTCCTC GGGAGGTGAC TATGAAGAAA GGAGATATTC TAACCCTGCT CAACAGCACC
    AACAAGGACT GGTGGAAGGT TGAAGTTAAC GATCGTCAGG GCTTTGTACC AGCTGCCTAT
    GTGAAAAAAC TAGATCCTGC CCAGTCTGCA TCCCGAGAGA ATCTTCTAGA AGAGCAAGGC
    AGCATAGCAT TGCGACAGGA GCAAATTGAC AACCAGACTC TCATTACTAA GGAAGTCGGC
    AGTGTATCTC TGCGTATGAA ACAGGTCGAA GAACTGTATC ATTCCCTTCT TGAGCTGGGA
    GAAAAACGTA AAGGCATGCT AGAAAAAAGC TGCAAGAAGT TTATGCTTTT CCGTGAGGCT
    AATGAGCTTC AACAGTGGAT CAATGAGAAG GAAGCTGCGC TCACTAATGA GGAAGTGGGT
    GCTGATTTGG AGCAGGTGGA GGTGCTACAG AAGAAGTTTG ATGATTTTCA GAAGGATTTA
    AAAGCTAATG AGTCACGACT GAAGGACATA AACAAGGTTG CAAATGACCT GGAGTCTGAA
    GGGCTGATGG CAGAGGAAGT GCAAGCAGTA GAGCACCAGG AAGTCTATGG AATGATGCCC
    AGAGATGAAA CTGATTCTAA GACAGCCTCT CCTTGGAAGT CTGCACGTAT GATGGTACAC
    ACAGTGGCAA CGTTTAACTC AATCAAGGAA CTGAATGAAC GCTGGAGATC CCTGCAGCAG
    TTGGCAGAGG AGCGAAGCCA GCTGTTGGGC AGCGCTCATG AAGTCCAGAG GTTCCACAGA
    GATGCTGATG AAACAAAAGA ATGGATAGAA GAGAAGAATC AAGCATTAAA TACCGACAAC
    TATGGACATG ACTTGGCCAG TGTGCAGGCT CTGCAGCGCA AGCATGAAGG CTTTGAGAGA
    GACTTGGCAG CTCTTGGAGA CAAGGTGAAT TCTCTTGGTG AAACTGCCCA GCGTCTGATC
    CAGTCACATC CAGAATCAGC TGAAGATCTT CAAGAAAAAT GTACTGAGTT GAACCAAGCC
    TGGAGTAGTC TGGGAAAACG TGCTGACCAA CGCAAAGAGA AGCTTGGAGA TTCTCATGAC
    CTGCAGCGTT TCCTCAGTGA TTTTAGAGAC CTTATGTCTT GGATCAATGG AATCCGGGGT
    CTGGTCTCCT CAGATGAACT TGCAAAAGAT GTGACTGGAG CTGAAGCTCT GTTGGAAAGG
    CATCAGGAGC ACCGTACTGA AATAGATGCA CGGGCTGGCA CTTTTCAGGC ATTTGAACAG
    TTTGGACAGC AGCTTCTGGC TCGTGGACAC TATGCCAGCC CAGAGATTAA GGAGAAACTG
    GATATTCTAG ATCAAGAACG GACAGACCTA GAGAAGGCCT GGGTCCAGCG CAGAATGATG
    CTAGACCAGT GCTTAGAACT ACAGCTGTTT CATCGGGATT GTGAACAAGC TGAAAACTGG
    ATGGCTGCCC GAGAGGCGTT CCTAAATACA GAAGACAAAG GAGACTCCTT AGACAGCGTG
    GAGGCACTCA TCAAGAAGCA TGAAGATTTC GATAAAGCAA TCAATGTCCA GGAAGAGAAA
    ATTGCTGTCT TGCAGTCTTT TGCTGACCAA CTGATTGCTG CTGATCATTA TGCAAAAGGA
    GTCATTGCTA ACAGACGCAA TGAGGTTCTG GACAGGTGGC GTCGTCTGAA GGCTCAGATG
    ATTGAGAAGA GATCTAAGCT GGGAGAATCT CAGACCCTCC AACAGTTCAG TCGTGATGTT
    GATGAAATAG AAGCTTGGAT CAGTGAAAAG CTTCAAACTG CAAGTGATGA GTCATATAAG
    GATCCCACAA ACATCCAGCT TTCCAAACTG CTGAGCAAGC ACCAGAAGCA CCAAGCCTTT
    GAAGCTGAGC TCCACGCCAA CGCAGATCGG ATTCGTGGAG TCATTGAAAT GGGGAACTCT
    CTTATTGAAA GAGGAGCGTG TGCTGGCAGC GAGGATGCTG TGAAGGCTCG GCTGGCTGCC
    CTGGCTGACC AATGGGAGTT CCTGGTCCAG AAGTCATCAG AGAAGAGTCA GAAACTGAAA
    GAAGCAAATA AACAGCAGAA TTTCAATACC GGCATCAAGG ACTTTGATTT CTGGCTTTCA
    GAGGTGGAAG CTTTGTTGGC ATCTGAAGAC TATGGGAAGG ACTTGGCATC AGTGAACAAC
    CTTCTGAAGA AGCACCAATT ACTGGAAGCT GATATATCTG CTCATGAGGA TCGGCTGAAG
    GACCTGAACA GCCAGGCTGA CAGTTTGATG ACCAGCAGTG CTTTCGATAC CTCCCAAGTA
    AAGGATAAAC GTGAAACTAT AAATGGGCGC TTCCAGAGAA TCAAGAGCAT GGCAGCTGCC
    CGCCGTGCGA AGCTCAACGA GTCGCACCGC TTGCATCAGT TCTTCCGTGA CATGGATGAT
    GAGGAGTCCT GGATCAAAGA GAAGAAACTG TTGGTGAGCT CAGAGGACTA TGGCAGAGAC
    CTGACTGGTG TGCAGAACCT GAGGAAGAAA CATAAGCGCT TGGAAGCAGA ATTAGCTGCC
    CATGAACCTG CTATCCAGGG TGTTCTAGAC ACTGGCAAGA AGCTTTCAGA TGATAACACA
    ATTGGGAAGG AGGAGATACA GCAAAGACTG GCTCAGTTTG TGGACCACTG GAAAGAGTTA
    AAACAGCTGG CAGCTGCTCG GGGGCAGCGT CTGGAGGAGT CCCTGGAGTA CCAACAGTTT
    GTAGCAAATG TTGAGGAAGA GGAGGCATGG ATCAACGAGA AAATGACATT GGTAGCCAGT
    GAGGATTATG GAGACACACT TGCTGCTATC CAGGGCTTGC TGAAGAAGCA TGAAGCATTC
    GAGACTGACT TTACTGTCCA CAAAGACAGA GTGAATGATG TTTGCGCTAA TGGAGAGGAT
    CTCATTAAAA AGAATAATCA CCATGTGGAG AACATTACTG CTAAGATGAA GGGCCTCAAG
    GGCAAGGTAT CAGATCTGGA GAAAGCCGCA GCTCAGAGGA AAGCCAAACT GGATGAGAAC
    TCTGCCTTCC TCCAGTTCAA CTGGAAAGCA GATGTGGTGG AGTCATGGAT AGGTGAGAAG
    GAAAACAGTC TGAAGACAGA TGATTATGGC CGTGACCTCT CCTCTGTGCA AACACTACTC
    ACCAAACAGG AAACCTTTGA TGCTGGACTT CAGGCTTTCC AGCAGGAGGG AATTGCAAAC
    ATCACTGCTC TGAAAGACCA GCTACTGGCA GCCAAACATA TCCAATCAAA GGCCATTGAG
    GTTCGTCACG CTTCCTTGAT GAAACGCTGG AATCAGCTGC TTGCTAATTC TGCAGCCAGG
    AAAAAGAAAC TCTTGGAGGC TCAGGAGCAC TTCAGAAAGG TTGAAGATCT CTTCTTGACT
    TTTGCCAAGA AGGCCTCTGC CTTCAACAGT TGGTTTGAGA ATGCTGAGGA GGACCTGACG
    GATCCCGTGC GCTGCAATTC CCTGGAAGAA ATCAAAGCAC TGCGAGAAGC CCACGATGCG
    TTCCGTTCCT CACTTAGCTC TGCCCAAGCT GACTTCAACC AGCTGGCAGA GCTTGATCGG
    CAGATCAAGA GCTTCCGTGT AGCCTCCAAC CCGTACACTT GGTTTACTAT GGAGGCTCTT
    GAAGAAACTT GGAGGAATCT GCAGAAAATT ATCAAGGAGC GTGAATTGGA GTTGCAGAAG
    GAGCAGCGGA GGCAGGAAGA AAATGACAAG TTGCGCCAGG AGTTTGCTCA GCATGCTAAT
    GCCTTCCACC AGTGGATTCA GGAGACAAGG ACTTACCTGC TAGATGGCAT AGCCTATCGT
    CGTGTCATTC GTGTCTATCA GTATGAAGTT GGGGATGATC TATCTGGAAG GTCCTGCATG
    GTGGAGGAGT CGGGAACACT GGAATCCCAG CTGGAAGCTA CTAAACGCAA GCACCAGGAG
    ATCCGGGCTA TGAGGAGCCA GCTGAAGAAG ATTGAGGACC TTGGAGCAGC CATGGAAGAG
    GCACTTATCT TGGACAACAA ATACACAGAG CACAGCACCG TGGGGCTGGC GCAGCAGTGG
    GACCAGCTTG ACCAGCTGGG AATGAGAATG CAGCACAATC TGGAACAGCA GATTCAAGCT
    CGAAACACAA CTGGAGTCAC AGAGGAGGCC CTGAAGGAGT TCAGCATGAT GTTCAAGCAC
    TTTGACAAGG ACAAATCTGG ACGTCTTAAT CACCAGGAGT TTAAGTCTTG CTTGCGCTCC
    CTTGGCTATG ACCTGCCCAT GGTTGAGGAA GGAGAGCCTG ACCCCGAATT TGAGTCTATT
    CTTGACACTG TTGATCCCAA CAGGGATGGC CACGTCTCGC TGCAGGAGTA CATGGCGTTC
    ATGATCAGCA GGGAAACAGA GAACGTGAAA TCCAGCGAGG AGATTGAGAG TGCTTTCCGT
    GCCCTCAGCT CGGAGGGGAA GCCTTACGTG ACCAAGGAGG AGCTCTACCA GAACCTGACC
    CGGGAGCAGG CCGACTACTG CATTTCTCAC ATGAAGCCCT ACATGGATGG CAAGGGCAGA
    GAACTTCCTT CTGCCTACGA CTACATAGAA TTTACACGTT CACTCTTTGT GAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa43315
    Clone ID Related Accession (Same CDS sequence) XM_015279289.1 , XM_015279289.2
    Accession Version XM_015279289.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7497bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015279289.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGGATCCAA GTGGGGTAAA AGTGTTGGAA ACAGCAGAAG ATATCCAAGA ACGGCGTCAG 
    CAGGTTTTGG ACCGTTACCA CAGGTTCAAG GAGCTGTCTT CTCTGAGGCG CCAAAAACTC
    GAAGATTCCT ATCGGTTCCA GTTCTTCCAG CGCGATGCAG ATGAGCTGGA AAAATGGATC
    CAAGAGAAAC TCCAGATAGC ATCTGATGAA AATTACAAAG ACCCAAGCAA TTTACAGGGG
    AAGCTGCAGA AGCACCAGGC CTTTGAAGCT GAGGTGCAGG CCAATTCAGG GGCTATCGTT
    AAGCTGGATG AGACTGGAAA TCAGATGATC AATGAAGGCC ATTTTGCATC TGAAACCATA
    AGAACTCGTC TACAGGAGCT GCACCGACTA TGGGAGTTAC TGCTGGAAAA AATGAGAGAG
    AAGGGAGTGA AGCTGTTGCA AGCACAGAAG CTGGTGCAAT TCTTACGGGA ATGTGAAGAT
    GTCATGGACT GGATCAATGA CAAGGAAGCA ATAGTGACCT CAGAAGAGCT TGGACAGGAC
    TTAGAGCATG TTGAGGTTTT GCAGAAGAAA TTTGAAGAGT TCCAAACAGA TCTGGCAGCT
    CATGAGGAGA GAGTGAATGA AGTGAACCAG TTCGCTGGCA AACTTATCCA GGAACAGCAC
    CCTGAGGAGG AACTTATAAA GTCCAAACAG GATGAAGTAA ATGCAAGCTG GCAGCGTCTT
    AAGGGGCTTG CCCAACAGAG GCAAGGAAAA CTCTTTGGGG CAGCTGAAGT TCAGCGCTTC
    AACAGGGATG TGGATGAAAC TATCAGCTGG ATTAAGGAGA AAGGGCAGTT GATGGCCTCA
    GATGACTTTG GCAGAGACTT GGCCAGCGTG CAAGCTTTAC TACGTAAGCA TGAAGGCCTG
    GAAAGAGATC TTGCAGCTTT GGAGGATAAG GTTAAGGCCC TCTGTGCAGA AGCTGACCGT
    TTGCAGCAGT CTCACCCTAT AAATGCTTCT CAAATTCAAG TGAAACGGGA GGAACTGATT
    GCCAACTGGG AGCAGATCCG AACTCTGGCA GCAGAGAGGC ATGCTCGCCT TAATGACTCC
    TACAGGTTGC AGCGCTTTCT TGCAGATTTC CGGGACCTCA CTAGCTGGGT AACTGAGATG
    AAGGCTCTGA TAAATGCTGA TGAGCTTGCC AATGATGTGG CTGGAGCAGA AGCCCTTCTA
    GATAGACATC AGGAACATAA GGGAGAAATT GATGCTCATG AGGATAGCTT CAGATCTGCT
    GATGAGTCTG GGCAGGCTTT GCTTGCAGCT GGGCACTATG CTTCTGATGA AGTTAAAGAA
    AAGCTGACTA TCCTCTCAGA TGAAAGATCA GCCTTGCTGG AACTATGGGA GCTTCGCAGG
    CAACAGTATG AGCAGTGCAT GGATCTGCAG CTTTTCTACA GAGATACTGA ACAAGTTGAC
    AACTGGATGA GCAAACAAGA AGCTTTTCTG CTGAACGAAG ACCTTGGTGA TTCTCTGGAT
    AGCGTGGAGG CTCTTCTAAA GAAGCATGAA GATTTTGAGA AATCCCTAAG TGCTCAAGAG
    GAGAAGATCA CAGCACTGGA TGAGTTTGCT ACTAAACTGA TTCAAAATAA CCATTATGCC
    ATGGATGACG TTGCTACGCG CAGAGATGCT CTTCTGAGTC GCCGAAATGC TCTTCATGAA
    AGAGCCATGA AGCGCCGTGC CCAACTGGCA GACTCTTTCC ATCTCCAGCA GTTTTTCAGG
    GACTCTGATG AACTGAAGAG TTGGGTTAAT GAAAAAATGA AAACTGCTAC CGATGAGGCT
    TACAAGGATC CATCCAACTT GCAAGGTAAA GTTCAGAAGC ATCAGGCTTT TGAAGCAGAG
    CTTTCTGCTA ATCAGAGTCG TATTGATGCA CTGGAGAAAG CTGGCCAGAA GTTGATTGAT
    GTCAATCACT ATGCATCTGA TGAGGTGGCA GCTCGCATGA ATGAAGTCAT CAGCTTGTGG
    AAGAAACTTC TGGAAGCCAC CGAGCTCAAA GGTATAAAGC TGCGTGAAGC CAATCAACAG
    CAGCAATTTA ATCGCAATGT AGAAGACATT GAGTTGTGGC TGTATGAAGT GGAGGGTCAC
    TTGGCTTCTG ATGATTATGG AAAAGATCTT ACCAGCGTTC AGAATCTTCA GAAGAAACAT
    GCCCTGCTAG AGGCAGATGT TGCTGCCCAT CAGGATCGGA TAGATGGCAT TACCATCCAG
    GCACGCCAGT TCCAAGATGC TGGGCACTTT GATGCTGACA ATATCAAGAA GAAACAAGAA
    GCTTTAGTAG CTCGTTATGA AGCTCTGAAG GATCCTATGG TGGCTCGCAA GCAGAAACTT
    GCAGATTCTC TTCGCCTGCA GCAGCTTTTC CGTGACATCG AGGATGAAGA GACCTGGATC
    AGGGAAAAGG AACCTATTGC AGCCTCAACA AACCGAGGCA AGGACTTAAT TGGTGTCCAG
    AATCTGCTAA AGAAGCACCA GGCTTTGCAG GCAGAAATTG CAGGCCATGA GCCTCGCATT
    AAAGCAGTCA CACAAAAAGG AAATGCGATG GTGGAAGAGG GACATTTTGC TGCTGAAGAT
    GTGAAGATCA AATTGAATGA ACTAAACCAA AAATGGGACT CTCTGAAAGC CAAGGCATCT
    CAACGGCGAC AGGATCTAGA GGATTCTCTG CAAGCTCAGC AGTATTTTGC TGATGCTAAT
    GAGGCAGAAT CATGGATGAG GGAAAAGGAG CCCATTGTAG GCAGCACGGA TTATGGAAAG
    GATGAAGACT CTGCTGAGGC TCTCCTGAAG AAGCATGAAG CTTTGATGTC TGATCTTTCT
    GCTTACGGCA GTAGCATACA GGCATTAAGG GAACAGGCCC AGTCCTGCAG GCAACAAGTT
    GCTCCCACTG ATGATGAAAC TGGAAAAGAG CTTGTGCTAG CACTCTATGA TTACCAAGAG
    AAGAGTCCTC GGGAGGTGAC TATGAAGAAA GGAGATATTC TAACCCTGCT CAACAGCACC
    AACAAGGACT GGTGGAAGGT TGAAGTTAAC GATCGTCAGG GCTTTGTACC AGCTGCCTAT
    GTGAAAAAAC TAGATCCTGC CCAGTCTGCA TCCCGAGAGA ATCTTCTAGA AGAGCAAGGC
    AGCATAGCAT TGCGACAGGA GCAAATTGAC AACCAGACTC TCATTACTAA GGAAGTCGGC
    AGTGTATCTC TGCGTATGAA ACAGGTCGAA GAACTGTATC ATTCCCTTCT TGAGCTGGGA
    GAAAAACGTA AAGGCATGCT AGAAAAAAGC TGCAAGAAGT TTATGCTTTT CCGTGAGGCT
    AATGAGCTTC AACAGTGGAT CAATGAGAAG GAAGCTGCGC TCACTAATGA GGAAGTGGGT
    GCTGATTTGG AGCAGGTGGA GGTGCTACAG AAGAAGTTTG ATGATTTTCA GAAGGATTTA
    AAAGCTAATG AGTCACGACT GAAGGACATA AACAAGGTTG CAAATGACCT GGAGTCTGAA
    GGGCTGATGG CAGAGGAAGT GCAAGCAGTA GAGCACCAGG AAGTCTATGG AATGATGCCC
    AGAGATGAAA CTGATTCTAA GACAGCCTCT CCTTGGAAGT CTGCACGTAT GATGGTACAC
    ACAGTGGCAA CGTTTAACTC AATCAAGGAA CTGAATGAAC GCTGGAGATC CCTGCAGCAG
    TTGGCAGAGG AGCGAAGCCA GCTGTTGGGC AGCGCTCATG AAGTCCAGAG GTTCCACAGA
    GATGCTGATG AAACAAAAGA ATGGATAGAA GAGAAGAATC AAGCATTAAA TACCGACAAC
    TATGGACATG ACTTGGCCAG TGTGCAGGCT CTGCAGCGCA AGCATGAAGG CTTTGAGAGA
    GACTTGGCAG CTCTTGGAGA CAAGGTGAAT TCTCTTGGTG AAACTGCCCA GCGTCTGATC
    CAGTCACATC CAGAATCAGC TGAAGATCTT CAAGAAAAAT GTACTGAGTT GAACCAAGCC
    TGGAGTAGTC TGGGAAAACG TGCTGACCAA CGCAAAGAGA AGCTTGGAGA TTCTCATGAC
    CTGCAGCGTT TCCTCAGTGA TTTTAGAGAC CTTATGTCTT GGATCAATGG AATCCGGGGT
    CTGGTCTCCT CAGATGAACT TGCAAAAGAT GTGACTGGAG CTGAAGCTCT GTTGGAAAGG
    CATCAGGAGC ACCGTACTGA AATAGATGCA CGGGCTGGCA CTTTTCAGGC ATTTGAACAG
    TTTGGACAGC AGCTTCTGGC TCGTGGACAC TATGCCAGCC CAGAGATTAA GGAGAAACTG
    GATATTCTAG ATCAAGAACG GACAGACCTA GAGAAGGCCT GGGTCCAGCG CAGAATGATG
    CTAGACCAGT GCTTAGAACT ACAGCTGTTT CATCGGGATT GTGAACAAGC TGAAAACTGG
    ATGGCTGCCC GAGAGGCGTT CCTAAATACA GAAGACAAAG GAGACTCCTT AGACAGCGTG
    GAGGCACTCA TCAAGAAGCA TGAAGATTTC GATAAAGCAA TCAATGTCCA GGAAGAGAAA
    ATTGCTGTCT TGCAGTCTTT TGCTGACCAA CTGATTGCTG CTGATCATTA TGCAAAAGGA
    GTCATTGCTA ACAGACGCAA TGAGGTTCTG GACAGGTGGC GTCGTCTGAA GGCTCAGATG
    ATTGAGAAGA GATCTAAGCT GGGAGAATCT CAGACCCTCC AACAGTTCAG TCGTGATGTT
    GATGAAATAG AAGCTTGGAT CAGTGAAAAG CTTCAAACTG CAAGTGATGA GTCATATAAG
    GATCCCACAA ACATCCAGCT TTCCAAACTG CTGAGCAAGC ACCAGAAGCA CCAAGCCTTT
    GAAGCTGAGC TCCACGCCAA CGCAGATCGG ATTCGTGGAG TCATTGAAAT GGGGAACTCT
    CTTATTGAAA GAGGAGCGTG TGCTGGCAGC GAGGATGCTG TGAAGGCTCG GCTGGCTGCC
    CTGGCTGACC AATGGGAGTT CCTGGTCCAG AAGTCATCAG AGAAGAGTCA GAAACTGAAA
    GAAGCAAATA AACAGCAGAA TTTCAATACC GGCATCAAGG ACTTTGATTT CTGGCTTTCA
    GAGGTGGAAG CTTTGTTGGC ATCTGAAGAC TATGGGAAGG ACTTGGCATC AGTGAACAAC
    CTTCTGAAGA AGCACCAATT ACTGGAAGCT GATATATCTG CTCATGAGGA TCGGCTGAAG
    GACCTGAACA GCCAGGCTGA CAGTTTGATG ACCAGCAGTG CTTTCGATAC CTCCCAAGTA
    AAGGATAAAC GTGAAACTAT AAATGGGCGC TTCCAGAGAA TCAAGAGCAT GGCAGCTGCC
    CGCCGTGCGA AGCTCAACGA GTCGCACCGC TTGCATCAGT TCTTCCGTGA CATGGATGAT
    GAGGAGTCCT GGATCAAAGA GAAGAAACTG TTGGTGAGCT CAGAGGACTA TGGCAGAGAC
    CTGACTGGTG TGCAGAACCT GAGGAAGAAA CATAAGCGCT TGGAAGCAGA ATTAGCTGCC
    CATGAACCTG CTATCCAGGG TGTTCTAGAC ACTGGCAAGA AGCTTTCAGA TGATAACACA
    ATTGGGAAGG AGGAGATACA GCAAAGACTG GCTCAGTTTG TGGACCACTG GAAAGAGTTA
    AAACAGCTGG CAGCTGCTCG GGGGCAGCGT CTGGAGGAGT CCCTGGAGTA CCAACAGTTT
    GTAGCAAATG TTGAGGAAGA GGAGGCATGG ATCAACGAGA AAATGACATT GGTAGCCAGT
    GAGGATTATG GAGACACACT TGCTGCTATC CAGGGCTTGC TGAAGAAGCA TGAAGCATTC
    GAGACTGACT TTACTGTCCA CAAAGACAGA GTGAATGATG TTTGCGCTAA TGGAGAGGAT
    CTCATTAAAA AGAATAATCA CCATGTGGAG AACATTACTG CTAAGATGAA GGGCCTCAAG
    GGCAAGGTAT CAGATCTGGA GAAAGCCGCA GCTCAGAGGA AAGCCAAACT GGATGAGAAC
    TCTGCCTTCC TCCAGTTCAA CTGGAAAGCA GATGTGGTGG AGTCATGGAT AGGTGAGAAG
    GAAAACAGTC TGAAGACAGA TGATTATGGC CGTGACCTCT CCTCTGTGCA AACACTACTC
    ACCAAACAGG AAACCTTTGA TGCTGGACTT CAGGCTTTCC AGCAGGAGGG AATTGCAAAC
    ATCACTGCTC TGAAAGACCA GCTACTGGCA GCCAAACATA TCCAATCAAA GGCCATTGAG
    GTTCGTCACG CTTCCTTGAT GAAACGCTGG AATCAGCTGC TTGCTAATTC TGCAGCCAGG
    AAAAAGAAAC TCTTGGAGGC TCAGGAGCAC TTCAGAAAGG TTGAAGATCT CTTCTTGACT
    TTTGCCAAGA AGGCCTCTGC CTTCAACAGT TGGTTTGAGA ATGCTGAGGA GGACCTGACG
    GATCCCGTGC GCTGCAATTC CCTGGAAGAA ATCAAAGCAC TGCGAGAAGC CCACGATGCG
    TTCCGTTCCT CACTTAGCTC TGCCCAAGCT GACTTCAACC AGCTGGCAGA GCTTGATCGG
    CAGATCAAGA GCTTCCGTGT AGCCTCCAAC CCGTACACTT GGTTTACTAT GGAGGCTCTT
    GAAGAAACTT GGAGGAATCT GCAGAAAATT ATCAAGGAGC GTGAATTGGA GTTGCAGAAG
    GAGCAGCGGA GGCAGGAAGA AAATGACAAG TTGCGCCAGG AGTTTGCTCA GCATGCTAAT
    GCCTTCCACC AGTGGATTCA GGAGACAAGG ACTTACCTGC TAGATGGCAT AGCCTATCGT
    CGTGTCATTC GTGTCTATCA GTATGAAGTT GGGGATGATC TATCTGGAAG GTCCTGCATG
    GTGGAGGAGT CGGGAACACT GGAATCCCAG CTGGAAGCTA CTAAACGCAA GCACCAGGAG
    ATCCGGGCTA TGAGGAGCCA GCTGAAGAAG ATTGAGGACC TTGGAGCAGC CATGGAAGAG
    GCACTTATCT TGGACAACAA ATACACAGAG CACAGCACCG TGGGGCTGGC GCAGCAGTGG
    GACCAGCTTG ACCAGCTGGG AATGAGAATG CAGCACAATC TGGAACAGCA GATTCAAGCT
    CGAAACACAA CTGGAGTCAC AGAGGAGGCC CTGAAGGAGT TCAGCATGAT GTTCAAGCAC
    TTTGACAAGG ACAAATCTGG ACGTCTTAAT CACCAGGAGT TTAAGTCTTG CTTGCGCTCC
    CTTGGCTATG ACCTGCCCAT GGTTGAGGAA GGAGAGCCTG ACCCCGAATT TGAGTCTATT
    CTTGACACTG TTGATCCCAA CAGGGATGGC CACGTCTCGC TGCAGGAGTA CATGGCGTTC
    ATGATCAGCA GGGAAACAGA GAACGTGAAA TCCAGCGAGG AGATTGAGAG TGCTTTCCGT
    GCCCTCAGCT CGGAGGGGAA GCCTTACGTG ACCAAGGAGG AGCTCTACCA GAACCTGACC
    CGGGAGCAGG CCGACTACTG CATTTCTCAC ATGAAGCCCT ACATGGATGG CAAGGGCAGA
    GAACTTCCTT CTGCCTACGA CTACATAGAA TTTACACGTT CACTCTTTGT GAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015134775.1
    CDS159..7655
    Translation

    Target ORF information:

    RefSeq Version XM_015279289.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015279289.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGGATCCAA GTGGGGTAAA AGTGTTGGAA ACAGCAGAAG ATATCCAAGA ACGGCGTCAG 
    CAGGTTTTGG ACCGTTACCA CAGGTTCAAG GAGCTGTCTT CTCTGAGGCG CCAAAAACTC
    GAAGATTCCT ATCGGTTCCA GTTCTTCCAG CGCGATGCAG ATGAGCTGGA AAAATGGATC
    CAAGAGAAAC TCCAGATAGC ATCTGATGAA AATTACAAAG ACCCAAGCAA TTTACAGGGG
    AAGCTGCAGA AGCACCAGGC CTTTGAAGCT GAGGTGCAGG CCAATTCAGG GGCTATCGTT
    AAGCTGGATG AGACTGGAAA TCAGATGATC AATGAAGGCC ATTTTGCATC TGAAACCATA
    AGAACTCGTC TACAGGAGCT GCACCGACTA TGGGAGTTAC TGCTGGAAAA AATGAGAGAG
    AAGGGAGTGA AGCTGTTGCA AGCACAGAAG CTGGTGCAAT TCTTACGGGA ATGTGAAGAT
    GTCATGGACT GGATCAATGA CAAGGAAGCA ATAGTGACCT CAGAAGAGCT TGGACAGGAC
    TTAGAGCATG TTGAGGTTTT GCAGAAGAAA TTTGAAGAGT TCCAAACAGA TCTGGCAGCT
    CATGAGGAGA GAGTGAATGA AGTGAACCAG TTCGCTGGCA AACTTATCCA GGAACAGCAC
    CCTGAGGAGG AACTTATAAA GTCCAAACAG GATGAAGTAA ATGCAAGCTG GCAGCGTCTT
    AAGGGGCTTG CCCAACAGAG GCAAGGAAAA CTCTTTGGGG CAGCTGAAGT TCAGCGCTTC
    AACAGGGATG TGGATGAAAC TATCAGCTGG ATTAAGGAGA AAGGGCAGTT GATGGCCTCA
    GATGACTTTG GCAGAGACTT GGCCAGCGTG CAAGCTTTAC TACGTAAGCA TGAAGGCCTG
    GAAAGAGATC TTGCAGCTTT GGAGGATAAG GTTAAGGCCC TCTGTGCAGA AGCTGACCGT
    TTGCAGCAGT CTCACCCTAT AAATGCTTCT CAAATTCAAG TGAAACGGGA GGAACTGATT
    GCCAACTGGG AGCAGATCCG AACTCTGGCA GCAGAGAGGC ATGCTCGCCT TAATGACTCC
    TACAGGTTGC AGCGCTTTCT TGCAGATTTC CGGGACCTCA CTAGCTGGGT AACTGAGATG
    AAGGCTCTGA TAAATGCTGA TGAGCTTGCC AATGATGTGG CTGGAGCAGA AGCCCTTCTA
    GATAGACATC AGGAACATAA GGGAGAAATT GATGCTCATG AGGATAGCTT CAGATCTGCT
    GATGAGTCTG GGCAGGCTTT GCTTGCAGCT GGGCACTATG CTTCTGATGA AGTTAAAGAA
    AAGCTGACTA TCCTCTCAGA TGAAAGATCA GCCTTGCTGG AACTATGGGA GCTTCGCAGG
    CAACAGTATG AGCAGTGCAT GGATCTGCAG CTTTTCTACA GAGATACTGA ACAAGTTGAC
    AACTGGATGA GCAAACAAGA AGCTTTTCTG CTGAACGAAG ACCTTGGTGA TTCTCTGGAT
    AGCGTGGAGG CTCTTCTAAA GAAGCATGAA GATTTTGAGA AATCCCTAAG TGCTCAAGAG
    GAGAAGATCA CAGCACTGGA TGAGTTTGCT ACTAAACTGA TTCAAAATAA CCATTATGCC
    ATGGATGACG TTGCTACGCG CAGAGATGCT CTTCTGAGTC GCCGAAATGC TCTTCATGAA
    AGAGCCATGA AGCGCCGTGC CCAACTGGCA GACTCTTTCC ATCTCCAGCA GTTTTTCAGG
    GACTCTGATG AACTGAAGAG TTGGGTTAAT GAAAAAATGA AAACTGCTAC CGATGAGGCT
    TACAAGGATC CATCCAACTT GCAAGGTAAA GTTCAGAAGC ATCAGGCTTT TGAAGCAGAG
    CTTTCTGCTA ATCAGAGTCG TATTGATGCA CTGGAGAAAG CTGGCCAGAA GTTGATTGAT
    GTCAATCACT ATGCATCTGA TGAGGTGGCA GCTCGCATGA ATGAAGTCAT CAGCTTGTGG
    AAGAAACTTC TGGAAGCCAC CGAGCTCAAA GGTATAAAGC TGCGTGAAGC CAATCAACAG
    CAGCAATTTA ATCGCAATGT AGAAGACATT GAGTTGTGGC TGTATGAAGT GGAGGGTCAC
    TTGGCTTCTG ATGATTATGG AAAAGATCTT ACCAGCGTTC AGAATCTTCA GAAGAAACAT
    GCCCTGCTAG AGGCAGATGT TGCTGCCCAT CAGGATCGGA TAGATGGCAT TACCATCCAG
    GCACGCCAGT TCCAAGATGC TGGGCACTTT GATGCTGACA ATATCAAGAA GAAACAAGAA
    GCTTTAGTAG CTCGTTATGA AGCTCTGAAG GATCCTATGG TGGCTCGCAA GCAGAAACTT
    GCAGATTCTC TTCGCCTGCA GCAGCTTTTC CGTGACATCG AGGATGAAGA GACCTGGATC
    AGGGAAAAGG AACCTATTGC AGCCTCAACA AACCGAGGCA AGGACTTAAT TGGTGTCCAG
    AATCTGCTAA AGAAGCACCA GGCTTTGCAG GCAGAAATTG CAGGCCATGA GCCTCGCATT
    AAAGCAGTCA CACAAAAAGG AAATGCGATG GTGGAAGAGG GACATTTTGC TGCTGAAGAT
    GTGAAGATCA AATTGAATGA ACTAAACCAA AAATGGGACT CTCTGAAAGC CAAGGCATCT
    CAACGGCGAC AGGATCTAGA GGATTCTCTG CAAGCTCAGC AGTATTTTGC TGATGCTAAT
    GAGGCAGAAT CATGGATGAG GGAAAAGGAG CCCATTGTAG GCAGCACGGA TTATGGAAAG
    GATGAAGACT CTGCTGAGGC TCTCCTGAAG AAGCATGAAG CTTTGATGTC TGATCTTTCT
    GCTTACGGCA GTAGCATACA GGCATTAAGG GAACAGGCCC AGTCCTGCAG GCAACAAGTT
    GCTCCCACTG ATGATGAAAC TGGAAAAGAG CTTGTGCTAG CACTCTATGA TTACCAAGAG
    AAGAGTCCTC GGGAGGTGAC TATGAAGAAA GGAGATATTC TAACCCTGCT CAACAGCACC
    AACAAGGACT GGTGGAAGGT TGAAGTTAAC GATCGTCAGG GCTTTGTACC AGCTGCCTAT
    GTGAAAAAAC TAGATCCTGC CCAGTCTGCA TCCCGAGAGA ATCTTCTAGA AGAGCAAGGC
    AGCATAGCAT TGCGACAGGA GCAAATTGAC AACCAGACTC TCATTACTAA GGAAGTCGGC
    AGTGTATCTC TGCGTATGAA ACAGGTCGAA GAACTGTATC ATTCCCTTCT TGAGCTGGGA
    GAAAAACGTA AAGGCATGCT AGAAAAAAGC TGCAAGAAGT TTATGCTTTT CCGTGAGGCT
    AATGAGCTTC AACAGTGGAT CAATGAGAAG GAAGCTGCGC TCACTAATGA GGAAGTGGGT
    GCTGATTTGG AGCAGGTGGA GGTGCTACAG AAGAAGTTTG ATGATTTTCA GAAGGATTTA
    AAAGCTAATG AGTCACGACT GAAGGACATA AACAAGGTTG CAAATGACCT GGAGTCTGAA
    GGGCTGATGG CAGAGGAAGT GCAAGCAGTA GAGCACCAGG AAGTCTATGG AATGATGCCC
    AGAGATGAAA CTGATTCTAA GACAGCCTCT CCTTGGAAGT CTGCACGTAT GATGGTACAC
    ACAGTGGCAA CGTTTAACTC AATCAAGGAA CTGAATGAAC GCTGGAGATC CCTGCAGCAG
    TTGGCAGAGG AGCGAAGCCA GCTGTTGGGC AGCGCTCATG AAGTCCAGAG GTTCCACAGA
    GATGCTGATG AAACAAAAGA ATGGATAGAA GAGAAGAATC AAGCATTAAA TACCGACAAC
    TATGGACATG ACTTGGCCAG TGTGCAGGCT CTGCAGCGCA AGCATGAAGG CTTTGAGAGA
    GACTTGGCAG CTCTTGGAGA CAAGGTGAAT TCTCTTGGTG AAACTGCCCA GCGTCTGATC
    CAGTCACATC CAGAATCAGC TGAAGATCTT CAAGAAAAAT GTACTGAGTT GAACCAAGCC
    TGGAGTAGTC TGGGAAAACG TGCTGACCAA CGCAAAGAGA AGCTTGGAGA TTCTCATGAC
    CTGCAGCGTT TCCTCAGTGA TTTTAGAGAC CTTATGTCTT GGATCAATGG AATCCGGGGT
    CTGGTCTCCT CAGATGAACT TGCAAAAGAT GTGACTGGAG CTGAAGCTCT GTTGGAAAGG
    CATCAGGAGC ACCGTACTGA AATAGATGCA CGGGCTGGCA CTTTTCAGGC ATTTGAACAG
    TTTGGACAGC AGCTTCTGGC TCGTGGACAC TATGCCAGCC CAGAGATTAA GGAGAAACTG
    GATATTCTAG ATCAAGAACG GACAGACCTA GAGAAGGCCT GGGTCCAGCG CAGAATGATG
    CTAGACCAGT GCTTAGAACT ACAGCTGTTT CATCGGGATT GTGAACAAGC TGAAAACTGG
    ATGGCTGCCC GAGAGGCGTT CCTAAATACA GAAGACAAAG GAGACTCCTT AGACAGCGTG
    GAGGCACTCA TCAAGAAGCA TGAAGATTTC GATAAAGCAA TCAATGTCCA GGAAGAGAAA
    ATTGCTGTCT TGCAGTCTTT TGCTGACCAA CTGATTGCTG CTGATCATTA TGCAAAAGGA
    GTCATTGCTA ACAGACGCAA TGAGGTTCTG GACAGGTGGC GTCGTCTGAA GGCTCAGATG
    ATTGAGAAGA GATCTAAGCT GGGAGAATCT CAGACCCTCC AACAGTTCAG TCGTGATGTT
    GATGAAATAG AAGCTTGGAT CAGTGAAAAG CTTCAAACTG CAAGTGATGA GTCATATAAG
    GATCCCACAA ACATCCAGCT TTCCAAACTG CTGAGCAAGC ACCAGAAGCA CCAAGCCTTT
    GAAGCTGAGC TCCACGCCAA CGCAGATCGG ATTCGTGGAG TCATTGAAAT GGGGAACTCT
    CTTATTGAAA GAGGAGCGTG TGCTGGCAGC GAGGATGCTG TGAAGGCTCG GCTGGCTGCC
    CTGGCTGACC AATGGGAGTT CCTGGTCCAG AAGTCATCAG AGAAGAGTCA GAAACTGAAA
    GAAGCAAATA AACAGCAGAA TTTCAATACC GGCATCAAGG ACTTTGATTT CTGGCTTTCA
    GAGGTGGAAG CTTTGTTGGC ATCTGAAGAC TATGGGAAGG ACTTGGCATC AGTGAACAAC
    CTTCTGAAGA AGCACCAATT ACTGGAAGCT GATATATCTG CTCATGAGGA TCGGCTGAAG
    GACCTGAACA GCCAGGCTGA CAGTTTGATG ACCAGCAGTG CTTTCGATAC CTCCCAAGTA
    AAGGATAAAC GTGAAACTAT AAATGGGCGC TTCCAGAGAA TCAAGAGCAT GGCAGCTGCC
    CGCCGTGCGA AGCTCAACGA GTCGCACCGC TTGCATCAGT TCTTCCGTGA CATGGATGAT
    GAGGAGTCCT GGATCAAAGA GAAGAAACTG TTGGTGAGCT CAGAGGACTA TGGCAGAGAC
    CTGACTGGTG TGCAGAACCT GAGGAAGAAA CATAAGCGCT TGGAAGCAGA ATTAGCTGCC
    CATGAACCTG CTATCCAGGG TGTTCTAGAC ACTGGCAAGA AGCTTTCAGA TGATAACACA
    ATTGGGAAGG AGGAGATACA GCAAAGACTG GCTCAGTTTG TGGACCACTG GAAAGAGTTA
    AAACAGCTGG CAGCTGCTCG GGGGCAGCGT CTGGAGGAGT CCCTGGAGTA CCAACAGTTT
    GTAGCAAATG TTGAGGAAGA GGAGGCATGG ATCAACGAGA AAATGACATT GGTAGCCAGT
    GAGGATTATG GAGACACACT TGCTGCTATC CAGGGCTTGC TGAAGAAGCA TGAAGCATTC
    GAGACTGACT TTACTGTCCA CAAAGACAGA GTGAATGATG TTTGCGCTAA TGGAGAGGAT
    CTCATTAAAA AGAATAATCA CCATGTGGAG AACATTACTG CTAAGATGAA GGGCCTCAAG
    GGCAAGGTAT CAGATCTGGA GAAAGCCGCA GCTCAGAGGA AAGCCAAACT GGATGAGAAC
    TCTGCCTTCC TCCAGTTCAA CTGGAAAGCA GATGTGGTGG AGTCATGGAT AGGTGAGAAG
    GAAAACAGTC TGAAGACAGA TGATTATGGC CGTGACCTCT CCTCTGTGCA AACACTACTC
    ACCAAACAGG AAACCTTTGA TGCTGGACTT CAGGCTTTCC AGCAGGAGGG AATTGCAAAC
    ATCACTGCTC TGAAAGACCA GCTACTGGCA GCCAAACATA TCCAATCAAA GGCCATTGAG
    GTTCGTCACG CTTCCTTGAT GAAACGCTGG AATCAGCTGC TTGCTAATTC TGCAGCCAGG
    AAAAAGAAAC TCTTGGAGGC TCAGGAGCAC TTCAGAAAGG TTGAAGATCT CTTCTTGACT
    TTTGCCAAGA AGGCCTCTGC CTTCAACAGT TGGTTTGAGA ATGCTGAGGA GGACCTGACG
    GATCCCGTGC GCTGCAATTC CCTGGAAGAA ATCAAAGCAC TGCGAGAAGC CCACGATGCG
    TTCCGTTCCT CACTTAGCTC TGCCCAAGCT GACTTCAACC AGCTGGCAGA GCTTGATCGG
    CAGATCAAGA GCTTCCGTGT AGCCTCCAAC CCGTACACTT GGTTTACTAT GGAGGCTCTT
    GAAGAAACTT GGAGGAATCT GCAGAAAATT ATCAAGGAGC GTGAATTGGA GTTGCAGAAG
    GAGCAGCGGA GGCAGGAAGA AAATGACAAG TTGCGCCAGG AGTTTGCTCA GCATGCTAAT
    GCCTTCCACC AGTGGATTCA GGAGACAAGG ACTTACCTGC TAGATGGCAT AGCCTATCGT
    CGTGTCATTC GTGTCTATCA GTATGAAGTT GGGGATGATC TATCTGGAAG GTCCTGCATG
    GTGGAGGAGT CGGGAACACT GGAATCCCAG CTGGAAGCTA CTAAACGCAA GCACCAGGAG
    ATCCGGGCTA TGAGGAGCCA GCTGAAGAAG ATTGAGGACC TTGGAGCAGC CATGGAAGAG
    GCACTTATCT TGGACAACAA ATACACAGAG CACAGCACCG TGGGGCTGGC GCAGCAGTGG
    GACCAGCTTG ACCAGCTGGG AATGAGAATG CAGCACAATC TGGAACAGCA GATTCAAGCT
    CGAAACACAA CTGGAGTCAC AGAGGAGGCC CTGAAGGAGT TCAGCATGAT GTTCAAGCAC
    TTTGACAAGG ACAAATCTGG ACGTCTTAAT CACCAGGAGT TTAAGTCTTG CTTGCGCTCC
    CTTGGCTATG ACCTGCCCAT GGTTGAGGAA GGAGAGCCTG ACCCCGAATT TGAGTCTATT
    CTTGACACTG TTGATCCCAA CAGGGATGGC CACGTCTCGC TGCAGGAGTA CATGGCGTTC
    ATGATCAGCA GGGAAACAGA GAACGTGAAA TCCAGCGAGG AGATTGAGAG TGCTTTCCGT
    GCCCTCAGCT CGGAGGGGAA GCCTTACGTG ACCAAGGAGG AGCTCTACCA GAACCTGACC
    CGGGAGCAGG CCGACTACTG CATTTCTCAC ATGAAGCCCT ACATGGATGG CAAGGGCAGA
    GAACTTCCTT CTGCCTACGA CTACATAGAA TTTACACGTT CACTCTTTGT GAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa43316
    Clone ID Related Accession (Same CDS sequence) XM_015279290.2 , XM_015279290.1
    Accession Version XM_015279290.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7473bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015279290.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
    TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG
    TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC
    TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT
    GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT
    GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG
    ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAA CTCGTCTACA GGAGCTGCAC
    CGACTATGGG AGTTACTGCT GGAAAAAATG AGAGAGAAGG GAGTGAAGCT GTTGCAAGCA
    CAGAAGCTGG TGCAATTCTT ACGGGAATGT GAAGATGTCA TGGACTGGAT CAATGACAAG
    GAAGCAATAG TGACCTCAGA AGAGCTTGGA CAGGACTTAG AGCATGTTGA GGTTTTGCAG
    AAGAAATTTG AAGAGTTCCA AACAGATCTG GCAGCTCATG AGGAGAGAGT GAATGAAGTG
    AACCAGTTCG CTGGCAAACT TATCCAGGAA CAGCACCCTG AGGAGGAACT TATAAAGTCC
    AAACAGGATG AAGTAAATGC AAGCTGGCAG CGTCTTAAGG GGCTTGCCCA ACAGAGGCAA
    GGAAAACTCT TTGGGGCAGC TGAAGTTCAG CGCTTCAACA GGGATGTGGA TGAAACTATC
    AGCTGGATTA AGGAGAAAGG GCAGTTGATG GCCTCAGATG ACTTTGGCAG AGACTTGGCC
    AGCGTGCAAG CTTTACTACG TAAGCATGAA GGCCTGGAAA GAGATCTTGC AGCTTTGGAG
    GATAAGGTTA AGGCCCTCTG TGCAGAAGCT GACCGTTTGC AGCAGTCTCA CCCTATAAAT
    GCTTCTCAAA TTCAAGTGAA ACGGGAGGAA CTGATTGCCA ACTGGGAGCA GATCCGAACT
    CTGGCAGCAG AGAGGCATGC TCGCCTTAAT GACTCCTACA GGTTGCAGCG CTTTCTTGCA
    GATTTCCGGG ACCTCACTAG CTGGGTAACT GAGATGAAGG CTCTGATAAA TGCTGATGAG
    CTTGCCAATG ATGTGGCTGG AGCAGAAGCC CTTCTAGATA GACATCAGGA ACATAAGGGA
    GAAATTGATG CTCATGAGGA TAGCTTCAGA TCTGCTGATG AGTCTGGGCA GGCTTTGCTT
    GCAGCTGGGC ACTATGCTTC TGATGAAGTT AAAGAAAAGC TGACTATCCT CTCAGATGAA
    AGATCAGCCT TGCTGGAACT ATGGGAGCTT CGCAGGCAAC AGTATGAGCA GTGCATGGAT
    CTGCAGCTTT TCTACAGAGA TACTGAACAA GTTGACAACT GGATGAGCAA ACAAGAAGCT
    TTTCTGCTGA ACGAAGACCT TGGTGATTCT CTGGATAGCG TGGAGGCTCT TCTAAAGAAG
    CATGAAGATT TTGAGAAATC CCTAAGTGCT CAAGAGGAGA AGATCACAGC ACTGGATGAG
    TTTGCTACTA AACTGATTCA AAATAACCAT TATGCCATGG ATGACGTTGC TACGCGCAGA
    GATGCTCTTC TGAGTCGCCG AAATGCTCTT CATGAAAGAG CCATGAAGCG CCGTGCCCAA
    CTGGCAGACT CTTTCCATCT CCAGCAGTTT TTCAGGGACT CTGATGAACT GAAGAGTTGG
    GTTAATGAAA AAATGAAAAC TGCTACCGAT GAGGCTTACA AGGATCCATC CAACTTGCAA
    GGTAAAGTTC AGAAGCATCA GGCTTTTGAA GCAGAGCTTT CTGCTAATCA GAGTCGTATT
    GATGCACTGG AGAAAGCTGG CCAGAAGTTG ATTGATGTCA ATCACTATGC ATCTGATGAG
    GTGGCAGCTC GCATGAATGA AGTCATCAGC TTGTGGAAGA AACTTCTGGA AGCCACCGAG
    CTCAAAGGTA TAAAGCTGCG TGAAGCCAAT CAACAGCAGC AATTTAATCG CAATGTAGAA
    GACATTGAGT TGTGGCTGTA TGAAGTGGAG GGTCACTTGG CTTCTGATGA TTATGGAAAA
    GATCTTACCA GCGTTCAGAA TCTTCAGAAG AAACATGCCC TGCTAGAGGC AGATGTTGCT
    GCCCATCAGG ATCGGATAGA TGGCATTACC ATCCAGGCAC GCCAGTTCCA AGATGCTGGG
    CACTTTGATG CTGACAATAT CAAGAAGAAA CAAGAAGCTT TAGTAGCTCG TTATGAAGCT
    CTGAAGGATC CTATGGTGGC TCGCAAGCAG AAACTTGCAG ATTCTCTTCG CCTGCAGCAG
    CTTTTCCGTG ACATCGAGGA TGAAGAGACC TGGATCAGGG AAAAGGAACC TATTGCAGCC
    TCAACAAACC GAGGCAAGGA CTTAATTGGT GTCCAGAATC TGCTAAAGAA GCACCAGGCT
    TTGCAGGCAG AAATTGCAGG CCATGAGCCT CGCATTAAAG CAGTCACACA AAAAGGAAAT
    GCGATGGTGG AAGAGGGACA TTTTGCTGCT GAAGATGTGA AGATCAAATT GAATGAACTA
    AACCAAAAAT GGGACTCTCT GAAAGCCAAG GCATCTCAAC GGCGACAGGA TCTAGAGGAT
    TCTCTGCAAG CTCAGCAGTA TTTTGCTGAT GCTAATGAGG CAGAATCATG GATGAGGGAA
    AAGGAGCCCA TTGTAGGCAG CACGGATTAT GGAAAGGATG AAGACTCTGC TGAGGCTCTC
    CTGAAGAAGC ATGAAGCTTT GATGTCTGAT CTTTCTGCTT ACGGCAGTAG CATACAGGCA
    TTAAGGGAAC AGGCCCAGTC CTGCAGGCAA CAAGTTGCTC CCACTGATGA TGAAACTGGA
    AAAGAGCTTG TGCTAGCACT CTATGATTAC CAAGAGAAGA GTCCTCGGGA GGTGACTATG
    AAGAAAGGAG ATATTCTAAC CCTGCTCAAC AGCACCAACA AGGACTGGTG GAAGGTTGAA
    GTTAACGATC GTCAGGGCTT TGTACCAGCT GCCTATGTGA AAAAACTAGA TCCTGCCCAG
    TCTGCATCCC GAGAGAATCT TCTAGAAGAG CAAGGCAGCA TAGCATTGCG ACAGGAGCAA
    ATTGACAACC AGTATCATTC CCTTCTTGAG CTGGGAGAAA AACGTAAAGG CATGCTAGAA
    AAAAGCTGCA AGAAGTTTAT GCTTTTCCGT GAGGCTAATG AGCTTCAACA GTGGATCAAT
    GAGAAGGAAG CTGCGCTCAC TAATGAGGAA GTGGGTGCTG ATTTGGAGCA GGTGGAGGTG
    CTACAGAAGA AGTTTGATGA TTTTCAGAAG GATTTAAAAG CTAATGAGTC ACGACTGAAG
    GACATAAACA AGGTTGCAAA TGACCTGGAG TCTGAAGGGC TGATGGCAGA GGAAGTGCAA
    GCAGTAGAGC ACCAGGAAGT CTATGGAATG ATGCCCAGAG ATGAAACTGA TTCTAAGACA
    GCCTCTCCTT GGAAGTCTGC ACGTATGATG GTACACACAG TGGCAACGTT TAACTCAATC
    AAGGAACTGA ATGAACGCTG GAGATCCCTG CAGCAGTTGG CAGAGGAGCG AAGCCAGCTG
    TTGGGCAGCG CTCATGAAGT CCAGAGGTTC CACAGAGATG CTGATGAAAC AAAAGAATGG
    ATAGAAGAGA AGAATCAAGC ATTAAATACC GACAACTATG GACATGACTT GGCCAGTGTG
    CAGGCTCTGC AGCGCAAGCA TGAAGGCTTT GAGAGAGACT TGGCAGCTCT TGGAGACAAG
    GTGAATTCTC TTGGTGAAAC TGCCCAGCGT CTGATCCAGT CACATCCAGA ATCAGCTGAA
    GATCTTCAAG AAAAATGTAC TGAGTTGAAC CAAGCCTGGA GTAGTCTGGG AAAACGTGCT
    GACCAACGCA AAGAGAAGCT TGGAGATTCT CATGACCTGC AGCGTTTCCT CAGTGATTTT
    AGAGACCTTA TGTCTTGGAT CAATGGAATC CGGGGTCTGG TCTCCTCAGA TGAACTTGCA
    AAAGATGTGA CTGGAGCTGA AGCTCTGTTG GAAAGGCATC AGGAGCACCG TACTGAAATA
    GATGCACGGG CTGGCACTTT TCAGGCATTT GAACAGTTTG GACAGCAGCT TCTGGCTCGT
    GGACACTATG CCAGCCCAGA GATTAAGGAG AAACTGGATA TTCTAGATCA AGAACGGACA
    GACCTAGAGA AGGCCTGGGT CCAGCGCAGA ATGATGCTAG ACCAGTGCTT AGAACTACAG
    CTGTTTCATC GGGATTGTGA ACAAGCTGAA AACTGGATGG CTGCCCGAGA GGCGTTCCTA
    AATACAGAAG ACAAAGGAGA CTCCTTAGAC AGCGTGGAGG CACTCATCAA GAAGCATGAA
    GATTTCGATA AAGCAATCAA TGTCCAGGAA GAGAAAATTG CTGTCTTGCA GTCTTTTGCT
    GACCAACTGA TTGCTGCTGA TCATTATGCA AAAGGAGTCA TTGCTAACAG ACGCAATGAG
    GTTCTGGACA GGTGGCGTCG TCTGAAGGCT CAGATGATTG AGAAGAGATC TAAGCTGGGA
    GAATCTCAGA CCCTCCAACA GTTCAGTCGT GATGTTGATG AAATAGAAGC TTGGATCAGT
    GAAAAGCTTC AAACTGCAAG TGATGAGTCA TATAAGGATC CCACAAACAT CCAGCTTTCC
    AAACTGCTGA GCAAGCACCA GAAGCACCAA GCCTTTGAAG CTGAGCTCCA CGCCAACGCA
    GATCGGATTC GTGGAGTCAT TGAAATGGGG AACTCTCTTA TTGAAAGAGG AGCGTGTGCT
    GGCAGCGAGG ATGCTGTGAA GGCTCGGCTG GCTGCCCTGG CTGACCAATG GGAGTTCCTG
    GTCCAGAAGT CATCAGAGAA GAGTCAGAAA CTGAAAGAAG CAAATAAACA GCAGAATTTC
    AATACCGGCA TCAAGGACTT TGATTTCTGG CTTTCAGAGG TGGAAGCTTT GTTGGCATCT
    GAAGACTATG GGAAGGACTT GGCATCAGTG AACAACCTTC TGAAGAAGCA CCAATTACTG
    GAAGCTGATA TATCTGCTCA TGAGGATCGG CTGAAGGACC TGAACAGCCA GGCTGACAGT
    TTGATGACCA GCAGTGCTTT CGATACCTCC CAAGTAAAGG ATAAACGTGA AACTATAAAT
    GGGCGCTTCC AGAGAATCAA GAGCATGGCA GCTGCCCGCC GTGCGAAGCT CAACGAGTCG
    CACCGCTTGC ATCAGTTCTT CCGTGACATG GATGATGAGG AGTCCTGGAT CAAAGAGAAG
    AAACTGTTGG TGAGCTCAGA GGACTATGGC AGAGACCTGA CTGGTGTGCA GAACCTGAGG
    AAGAAACATA AGCGCTTGGA AGCAGAATTA GCTGCCCATG AACCTGCTAT CCAGGGTGTT
    CTAGACACTG GCAAGAAGCT TTCAGATGAT AACACAATTG GGAAGGAGGA GATACAGCAA
    AGACTGGCTC AGTTTGTGGA CCACTGGAAA GAGTTAAAAC AGCTGGCAGC TGCTCGGGGG
    CAGCGTCTGG AGGAGTCCCT GGAGTACCAA CAGTTTGTAG CAAATGTTGA GGAAGAGGAG
    GCATGGATCA ACGAGAAAAT GACATTGGTA GCCAGTGAGG ATTATGGAGA CACACTTGCT
    GCTATCCAGG GCTTGCTGAA GAAGCATGAA GCATTCGAGA CTGACTTTAC TGTCCACAAA
    GACAGAGTGA ATGATGTTTG CGCTAATGGA GAGGATCTCA TTAAAAAGAA TAATCACCAT
    GTGGAGAACA TTACTGCTAA GATGAAGGGC CTCAAGGGCA AGGTATCAGA TCTGGAGAAA
    GCCGCAGCTC AGAGGAAAGC CAAACTGGAT GAGAACTCTG CCTTCCTCCA GTTCAACTGG
    AAAGCAGATG TGGTGGAGTC ATGGATAGGT GAGAAGGAAA ACAGTCTGAA GACAGATGAT
    TATGGCCGTG ACCTCTCCTC TGTGCAAACA CTACTCACCA AACAGGAAAC CTTTGATGCT
    GGACTTCAGG CTTTCCAGCA GGAGGGAATT GCAAACATCA CTGCTCTGAA AGACCAGCTA
    CTGGCAGCCA AACATATCCA ATCAAAGGCC ATTGAGGTTC GTCACGCTTC CTTGATGAAA
    CGCTGGAATC AGCTGCTTGC TAATTCTGCA GCCAGGAAAA AGAAACTCTT GGAGGCTCAG
    GAGCACTTCA GAAAGGTTGA AGATCTCTTC TTGACTTTTG CCAAGAAGGC CTCTGCCTTC
    AACAGTTGGT TTGAGAATGC TGAGGAGGAC CTGACGGATC CCGTGCGCTG CAATTCCCTG
    GAAGAAATCA AAGCACTGCG AGAAGCCCAC GATGCGTTCC GTTCCTCACT TAGCTCTGCC
    CAAGCTGACT TCAACCAGCT GGCAGAGCTT GATCGGCAGA TCAAGAGCTT CCGTGTAGCC
    TCCAACCCGT ACACTTGGTT TACTATGGAG GCTCTTGAAG AAACTTGGAG GAATCTGCAG
    AAAATTATCA AGGAGCGTGA ATTGGAGTTG CAGAAGGAGC AGCGGAGGCA GGAAGAAAAT
    GACAAGTTGC GCCAGGAGTT TGCTCAGCAT GCTAATGCCT TCCACCAGTG GATTCAGGAG
    ACAAGGACTT ACCTGCTAGA TGGCATAGCC TATCGTCGTG TCATTCGTGT CTATCAGTAT
    GAAGTTGGGG ATGATCTATC TGGAAGGTCC TGCATGGTGG AGGAGTCGGG AACACTGGAA
    TCCCAGCTGG AAGCTACTAA ACGCAAGCAC CAGGAGATCC GGGCTATGAG GAGCCAGCTG
    AAGAAGATTG AGGACCTTGG AGCAGCCATG GAAGAGGCAC TTATCTTGGA CAACAAATAC
    ACAGAGCACA GCACCGTGGG GCTGGCGCAG CAGTGGGACC AGCTTGACCA GCTGGGAATG
    AGAATGCAGC ACAATCTGGA ACAGCAGATT CAAGCTCGAA ACACAACTGG AGTCACAGAG
    GAGGCCCTGA AGGAGTTCAG CATGATGTTC AAGCACTTTG ACAAGGACAA ATCTGGACGT
    CTTAATCACC AGGAGTTTAA GTCTTGCTTG CGCTCCCTTG GCTATGACCT GCCCATGGTT
    GAGGAAGGAG AGCCTGACCC CGAATTTGAG TCTATTCTTG ACACTGTTGA TCCCAACAGG
    GATGGCCACG TCTCGCTGCA GGAGTACATG GCGTTCATGA TCAGCAGGGA AACAGAGAAC
    GTGAAATCCA GCGAGGAGAT TGAGAGTGCT TTCCGTGCCC TCAGCTCGGA GGGGAAGCCT
    TACGTGACCA AGGAGGAGCT CTACCAGAAC CTGACCCGGG AGCAGGCCGA CTACTGCATT
    TCTCACATGA AGCCCTACAT GGATGGCAAG GGCAGAGAAC TTCCTTCTGC CTACGACTAC
    ATAGAATTTA CACGTTCACT CTTTGTGAAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015134776.1
    CDS13..7485
    Translation

    Target ORF information:

    RefSeq Version XM_015279290.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015279290.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
    TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG
    TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC
    TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT
    GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT
    GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG
    ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAA CTCGTCTACA GGAGCTGCAC
    CGACTATGGG AGTTACTGCT GGAAAAAATG AGAGAGAAGG GAGTGAAGCT GTTGCAAGCA
    CAGAAGCTGG TGCAATTCTT ACGGGAATGT GAAGATGTCA TGGACTGGAT CAATGACAAG
    GAAGCAATAG TGACCTCAGA AGAGCTTGGA CAGGACTTAG AGCATGTTGA GGTTTTGCAG
    AAGAAATTTG AAGAGTTCCA AACAGATCTG GCAGCTCATG AGGAGAGAGT GAATGAAGTG
    AACCAGTTCG CTGGCAAACT TATCCAGGAA CAGCACCCTG AGGAGGAACT TATAAAGTCC
    AAACAGGATG AAGTAAATGC AAGCTGGCAG CGTCTTAAGG GGCTTGCCCA ACAGAGGCAA
    GGAAAACTCT TTGGGGCAGC TGAAGTTCAG CGCTTCAACA GGGATGTGGA TGAAACTATC
    AGCTGGATTA AGGAGAAAGG GCAGTTGATG GCCTCAGATG ACTTTGGCAG AGACTTGGCC
    AGCGTGCAAG CTTTACTACG TAAGCATGAA GGCCTGGAAA GAGATCTTGC AGCTTTGGAG
    GATAAGGTTA AGGCCCTCTG TGCAGAAGCT GACCGTTTGC AGCAGTCTCA CCCTATAAAT
    GCTTCTCAAA TTCAAGTGAA ACGGGAGGAA CTGATTGCCA ACTGGGAGCA GATCCGAACT
    CTGGCAGCAG AGAGGCATGC TCGCCTTAAT GACTCCTACA GGTTGCAGCG CTTTCTTGCA
    GATTTCCGGG ACCTCACTAG CTGGGTAACT GAGATGAAGG CTCTGATAAA TGCTGATGAG
    CTTGCCAATG ATGTGGCTGG AGCAGAAGCC CTTCTAGATA GACATCAGGA ACATAAGGGA
    GAAATTGATG CTCATGAGGA TAGCTTCAGA TCTGCTGATG AGTCTGGGCA GGCTTTGCTT
    GCAGCTGGGC ACTATGCTTC TGATGAAGTT AAAGAAAAGC TGACTATCCT CTCAGATGAA
    AGATCAGCCT TGCTGGAACT ATGGGAGCTT CGCAGGCAAC AGTATGAGCA GTGCATGGAT
    CTGCAGCTTT TCTACAGAGA TACTGAACAA GTTGACAACT GGATGAGCAA ACAAGAAGCT
    TTTCTGCTGA ACGAAGACCT TGGTGATTCT CTGGATAGCG TGGAGGCTCT TCTAAAGAAG
    CATGAAGATT TTGAGAAATC CCTAAGTGCT CAAGAGGAGA AGATCACAGC ACTGGATGAG
    TTTGCTACTA AACTGATTCA AAATAACCAT TATGCCATGG ATGACGTTGC TACGCGCAGA
    GATGCTCTTC TGAGTCGCCG AAATGCTCTT CATGAAAGAG CCATGAAGCG CCGTGCCCAA
    CTGGCAGACT CTTTCCATCT CCAGCAGTTT TTCAGGGACT CTGATGAACT GAAGAGTTGG
    GTTAATGAAA AAATGAAAAC TGCTACCGAT GAGGCTTACA AGGATCCATC CAACTTGCAA
    GGTAAAGTTC AGAAGCATCA GGCTTTTGAA GCAGAGCTTT CTGCTAATCA GAGTCGTATT
    GATGCACTGG AGAAAGCTGG CCAGAAGTTG ATTGATGTCA ATCACTATGC ATCTGATGAG
    GTGGCAGCTC GCATGAATGA AGTCATCAGC TTGTGGAAGA AACTTCTGGA AGCCACCGAG
    CTCAAAGGTA TAAAGCTGCG TGAAGCCAAT CAACAGCAGC AATTTAATCG CAATGTAGAA
    GACATTGAGT TGTGGCTGTA TGAAGTGGAG GGTCACTTGG CTTCTGATGA TTATGGAAAA
    GATCTTACCA GCGTTCAGAA TCTTCAGAAG AAACATGCCC TGCTAGAGGC AGATGTTGCT
    GCCCATCAGG ATCGGATAGA TGGCATTACC ATCCAGGCAC GCCAGTTCCA AGATGCTGGG
    CACTTTGATG CTGACAATAT CAAGAAGAAA CAAGAAGCTT TAGTAGCTCG TTATGAAGCT
    CTGAAGGATC CTATGGTGGC TCGCAAGCAG AAACTTGCAG ATTCTCTTCG CCTGCAGCAG
    CTTTTCCGTG ACATCGAGGA TGAAGAGACC TGGATCAGGG AAAAGGAACC TATTGCAGCC
    TCAACAAACC GAGGCAAGGA CTTAATTGGT GTCCAGAATC TGCTAAAGAA GCACCAGGCT
    TTGCAGGCAG AAATTGCAGG CCATGAGCCT CGCATTAAAG CAGTCACACA AAAAGGAAAT
    GCGATGGTGG AAGAGGGACA TTTTGCTGCT GAAGATGTGA AGATCAAATT GAATGAACTA
    AACCAAAAAT GGGACTCTCT GAAAGCCAAG GCATCTCAAC GGCGACAGGA TCTAGAGGAT
    TCTCTGCAAG CTCAGCAGTA TTTTGCTGAT GCTAATGAGG CAGAATCATG GATGAGGGAA
    AAGGAGCCCA TTGTAGGCAG CACGGATTAT GGAAAGGATG AAGACTCTGC TGAGGCTCTC
    CTGAAGAAGC ATGAAGCTTT GATGTCTGAT CTTTCTGCTT ACGGCAGTAG CATACAGGCA
    TTAAGGGAAC AGGCCCAGTC CTGCAGGCAA CAAGTTGCTC CCACTGATGA TGAAACTGGA
    AAAGAGCTTG TGCTAGCACT CTATGATTAC CAAGAGAAGA GTCCTCGGGA GGTGACTATG
    AAGAAAGGAG ATATTCTAAC CCTGCTCAAC AGCACCAACA AGGACTGGTG GAAGGTTGAA
    GTTAACGATC GTCAGGGCTT TGTACCAGCT GCCTATGTGA AAAAACTAGA TCCTGCCCAG
    TCTGCATCCC GAGAGAATCT TCTAGAAGAG CAAGGCAGCA TAGCATTGCG ACAGGAGCAA
    ATTGACAACC AGTATCATTC CCTTCTTGAG CTGGGAGAAA AACGTAAAGG CATGCTAGAA
    AAAAGCTGCA AGAAGTTTAT GCTTTTCCGT GAGGCTAATG AGCTTCAACA GTGGATCAAT
    GAGAAGGAAG CTGCGCTCAC TAATGAGGAA GTGGGTGCTG ATTTGGAGCA GGTGGAGGTG
    CTACAGAAGA AGTTTGATGA TTTTCAGAAG GATTTAAAAG CTAATGAGTC ACGACTGAAG
    GACATAAACA AGGTTGCAAA TGACCTGGAG TCTGAAGGGC TGATGGCAGA GGAAGTGCAA
    GCAGTAGAGC ACCAGGAAGT CTATGGAATG ATGCCCAGAG ATGAAACTGA TTCTAAGACA
    GCCTCTCCTT GGAAGTCTGC ACGTATGATG GTACACACAG TGGCAACGTT TAACTCAATC
    AAGGAACTGA ATGAACGCTG GAGATCCCTG CAGCAGTTGG CAGAGGAGCG AAGCCAGCTG
    TTGGGCAGCG CTCATGAAGT CCAGAGGTTC CACAGAGATG CTGATGAAAC AAAAGAATGG
    ATAGAAGAGA AGAATCAAGC ATTAAATACC GACAACTATG GACATGACTT GGCCAGTGTG
    CAGGCTCTGC AGCGCAAGCA TGAAGGCTTT GAGAGAGACT TGGCAGCTCT TGGAGACAAG
    GTGAATTCTC TTGGTGAAAC TGCCCAGCGT CTGATCCAGT CACATCCAGA ATCAGCTGAA
    GATCTTCAAG AAAAATGTAC TGAGTTGAAC CAAGCCTGGA GTAGTCTGGG AAAACGTGCT
    GACCAACGCA AAGAGAAGCT TGGAGATTCT CATGACCTGC AGCGTTTCCT CAGTGATTTT
    AGAGACCTTA TGTCTTGGAT CAATGGAATC CGGGGTCTGG TCTCCTCAGA TGAACTTGCA
    AAAGATGTGA CTGGAGCTGA AGCTCTGTTG GAAAGGCATC AGGAGCACCG TACTGAAATA
    GATGCACGGG CTGGCACTTT TCAGGCATTT GAACAGTTTG GACAGCAGCT TCTGGCTCGT
    GGACACTATG CCAGCCCAGA GATTAAGGAG AAACTGGATA TTCTAGATCA AGAACGGACA
    GACCTAGAGA AGGCCTGGGT CCAGCGCAGA ATGATGCTAG ACCAGTGCTT AGAACTACAG
    CTGTTTCATC GGGATTGTGA ACAAGCTGAA AACTGGATGG CTGCCCGAGA GGCGTTCCTA
    AATACAGAAG ACAAAGGAGA CTCCTTAGAC AGCGTGGAGG CACTCATCAA GAAGCATGAA
    GATTTCGATA AAGCAATCAA TGTCCAGGAA GAGAAAATTG CTGTCTTGCA GTCTTTTGCT
    GACCAACTGA TTGCTGCTGA TCATTATGCA AAAGGAGTCA TTGCTAACAG ACGCAATGAG
    GTTCTGGACA GGTGGCGTCG TCTGAAGGCT CAGATGATTG AGAAGAGATC TAAGCTGGGA
    GAATCTCAGA CCCTCCAACA GTTCAGTCGT GATGTTGATG AAATAGAAGC TTGGATCAGT
    GAAAAGCTTC AAACTGCAAG TGATGAGTCA TATAAGGATC CCACAAACAT CCAGCTTTCC
    AAACTGCTGA GCAAGCACCA GAAGCACCAA GCCTTTGAAG CTGAGCTCCA CGCCAACGCA
    GATCGGATTC GTGGAGTCAT TGAAATGGGG AACTCTCTTA TTGAAAGAGG AGCGTGTGCT
    GGCAGCGAGG ATGCTGTGAA GGCTCGGCTG GCTGCCCTGG CTGACCAATG GGAGTTCCTG
    GTCCAGAAGT CATCAGAGAA GAGTCAGAAA CTGAAAGAAG CAAATAAACA GCAGAATTTC
    AATACCGGCA TCAAGGACTT TGATTTCTGG CTTTCAGAGG TGGAAGCTTT GTTGGCATCT
    GAAGACTATG GGAAGGACTT GGCATCAGTG AACAACCTTC TGAAGAAGCA CCAATTACTG
    GAAGCTGATA TATCTGCTCA TGAGGATCGG CTGAAGGACC TGAACAGCCA GGCTGACAGT
    TTGATGACCA GCAGTGCTTT CGATACCTCC CAAGTAAAGG ATAAACGTGA AACTATAAAT
    GGGCGCTTCC AGAGAATCAA GAGCATGGCA GCTGCCCGCC GTGCGAAGCT CAACGAGTCG
    CACCGCTTGC ATCAGTTCTT CCGTGACATG GATGATGAGG AGTCCTGGAT CAAAGAGAAG
    AAACTGTTGG TGAGCTCAGA GGACTATGGC AGAGACCTGA CTGGTGTGCA GAACCTGAGG
    AAGAAACATA AGCGCTTGGA AGCAGAATTA GCTGCCCATG AACCTGCTAT CCAGGGTGTT
    CTAGACACTG GCAAGAAGCT TTCAGATGAT AACACAATTG GGAAGGAGGA GATACAGCAA
    AGACTGGCTC AGTTTGTGGA CCACTGGAAA GAGTTAAAAC AGCTGGCAGC TGCTCGGGGG
    CAGCGTCTGG AGGAGTCCCT GGAGTACCAA CAGTTTGTAG CAAATGTTGA GGAAGAGGAG
    GCATGGATCA ACGAGAAAAT GACATTGGTA GCCAGTGAGG ATTATGGAGA CACACTTGCT
    GCTATCCAGG GCTTGCTGAA GAAGCATGAA GCATTCGAGA CTGACTTTAC TGTCCACAAA
    GACAGAGTGA ATGATGTTTG CGCTAATGGA GAGGATCTCA TTAAAAAGAA TAATCACCAT
    GTGGAGAACA TTACTGCTAA GATGAAGGGC CTCAAGGGCA AGGTATCAGA TCTGGAGAAA
    GCCGCAGCTC AGAGGAAAGC CAAACTGGAT GAGAACTCTG CCTTCCTCCA GTTCAACTGG
    AAAGCAGATG TGGTGGAGTC ATGGATAGGT GAGAAGGAAA ACAGTCTGAA GACAGATGAT
    TATGGCCGTG ACCTCTCCTC TGTGCAAACA CTACTCACCA AACAGGAAAC CTTTGATGCT
    GGACTTCAGG CTTTCCAGCA GGAGGGAATT GCAAACATCA CTGCTCTGAA AGACCAGCTA
    CTGGCAGCCA AACATATCCA ATCAAAGGCC ATTGAGGTTC GTCACGCTTC CTTGATGAAA
    CGCTGGAATC AGCTGCTTGC TAATTCTGCA GCCAGGAAAA AGAAACTCTT GGAGGCTCAG
    GAGCACTTCA GAAAGGTTGA AGATCTCTTC TTGACTTTTG CCAAGAAGGC CTCTGCCTTC
    AACAGTTGGT TTGAGAATGC TGAGGAGGAC CTGACGGATC CCGTGCGCTG CAATTCCCTG
    GAAGAAATCA AAGCACTGCG AGAAGCCCAC GATGCGTTCC GTTCCTCACT TAGCTCTGCC
    CAAGCTGACT TCAACCAGCT GGCAGAGCTT GATCGGCAGA TCAAGAGCTT CCGTGTAGCC
    TCCAACCCGT ACACTTGGTT TACTATGGAG GCTCTTGAAG AAACTTGGAG GAATCTGCAG
    AAAATTATCA AGGAGCGTGA ATTGGAGTTG CAGAAGGAGC AGCGGAGGCA GGAAGAAAAT
    GACAAGTTGC GCCAGGAGTT TGCTCAGCAT GCTAATGCCT TCCACCAGTG GATTCAGGAG
    ACAAGGACTT ACCTGCTAGA TGGCATAGCC TATCGTCGTG TCATTCGTGT CTATCAGTAT
    GAAGTTGGGG ATGATCTATC TGGAAGGTCC TGCATGGTGG AGGAGTCGGG AACACTGGAA
    TCCCAGCTGG AAGCTACTAA ACGCAAGCAC CAGGAGATCC GGGCTATGAG GAGCCAGCTG
    AAGAAGATTG AGGACCTTGG AGCAGCCATG GAAGAGGCAC TTATCTTGGA CAACAAATAC
    ACAGAGCACA GCACCGTGGG GCTGGCGCAG CAGTGGGACC AGCTTGACCA GCTGGGAATG
    AGAATGCAGC ACAATCTGGA ACAGCAGATT CAAGCTCGAA ACACAACTGG AGTCACAGAG
    GAGGCCCTGA AGGAGTTCAG CATGATGTTC AAGCACTTTG ACAAGGACAA ATCTGGACGT
    CTTAATCACC AGGAGTTTAA GTCTTGCTTG CGCTCCCTTG GCTATGACCT GCCCATGGTT
    GAGGAAGGAG AGCCTGACCC CGAATTTGAG TCTATTCTTG ACACTGTTGA TCCCAACAGG
    GATGGCCACG TCTCGCTGCA GGAGTACATG GCGTTCATGA TCAGCAGGGA AACAGAGAAC
    GTGAAATCCA GCGAGGAGAT TGAGAGTGCT TTCCGTGCCC TCAGCTCGGA GGGGAAGCCT
    TACGTGACCA AGGAGGAGCT CTACCAGAAC CTGACCCGGG AGCAGGCCGA CTACTGCATT
    TCTCACATGA AGCCCTACAT GGATGGCAAG GGCAGAGAAC TTCCTTCTGC CTACGACTAC
    ATAGAATTTA CACGTTCACT CTTTGTGAAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa43316
    Clone ID Related Accession (Same CDS sequence) XM_015279290.2 , XM_015279290.1
    Accession Version XM_015279290.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7473bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
    TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG
    TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC
    TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT
    GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT
    GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG
    ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAA CTCGTCTACA GGAGCTGCAC
    CGACTATGGG AGTTACTGCT GGAAAAAATG AGAGAGAAGG GAGTGAAGCT GTTGCAAGCA
    CAGAAGCTGG TGCAATTCTT ACGGGAATGT GAAGATGTCA TGGACTGGAT CAATGACAAG
    GAAGCAATAG TGACCTCAGA AGAGCTTGGA CAGGACTTAG AGCATGTTGA GGTTTTGCAG
    AAGAAATTTG AAGAGTTCCA AACAGATCTG GCAGCTCATG AGGAGAGAGT GAATGAAGTG
    AACCAGTTCG CTGGCAAACT TATCCAGGAA CAGCACCCTG AGGAGGAACT TATAAAGTCC
    AAACAGGATG AAGTAAATGC AAGCTGGCAG CGTCTTAAGG GGCTTGCCCA ACAGAGGCAA
    GGAAAACTCT TTGGGGCAGC TGAAGTTCAG CGCTTCAACA GGGATGTGGA TGAAACTATC
    AGCTGGATTA AGGAGAAAGG GCAGTTGATG GCCTCAGATG ACTTTGGCAG AGACTTGGCC
    AGCGTGCAAG CTTTACTACG TAAGCATGAA GGCCTGGAAA GAGATCTTGC AGCTTTGGAG
    GATAAGGTTA AGGCCCTCTG TGCAGAAGCT GACCGTTTGC AGCAGTCTCA CCCTATAAAT
    GCTTCTCAAA TTCAAGTGAA ACGGGAGGAA CTGATTGCCA ACTGGGAGCA GATCCGAACT
    CTGGCAGCAG AGAGGCATGC TCGCCTTAAT GACTCCTACA GGTTGCAGCG CTTTCTTGCA
    GATTTCCGGG ACCTCACTAG CTGGGTAACT GAGATGAAGG CTCTGATAAA TGCTGATGAG
    CTTGCCAATG ATGTGGCTGG AGCAGAAGCC CTTCTAGATA GACATCAGGA ACATAAGGGA
    GAAATTGATG CTCATGAGGA TAGCTTCAGA TCTGCTGATG AGTCTGGGCA GGCTTTGCTT
    GCAGCTGGGC ACTATGCTTC TGATGAAGTT AAAGAAAAGC TGACTATCCT CTCAGATGAA
    AGATCAGCCT TGCTGGAACT ATGGGAGCTT CGCAGGCAAC AGTATGAGCA GTGCATGGAT
    CTGCAGCTTT TCTACAGAGA TACTGAACAA GTTGACAACT GGATGAGCAA ACAAGAAGCT
    TTTCTGCTGA ACGAAGACCT TGGTGATTCT CTGGATAGCG TGGAGGCTCT TCTAAAGAAG
    CATGAAGATT TTGAGAAATC CCTAAGTGCT CAAGAGGAGA AGATCACAGC ACTGGATGAG
    TTTGCTACTA AACTGATTCA AAATAACCAT TATGCCATGG ATGACGTTGC TACGCGCAGA
    GATGCTCTTC TGAGTCGCCG AAATGCTCTT CATGAAAGAG CCATGAAGCG CCGTGCCCAA
    CTGGCAGACT CTTTCCATCT CCAGCAGTTT TTCAGGGACT CTGATGAACT GAAGAGTTGG
    GTTAATGAAA AAATGAAAAC TGCTACCGAT GAGGCTTACA AGGATCCATC CAACTTGCAA
    GGTAAAGTTC AGAAGCATCA GGCTTTTGAA GCAGAGCTTT CTGCTAATCA GAGTCGTATT
    GATGCACTGG AGAAAGCTGG CCAGAAGTTG ATTGATGTCA ATCACTATGC ATCTGATGAG
    GTGGCAGCTC GCATGAATGA AGTCATCAGC TTGTGGAAGA AACTTCTGGA AGCCACCGAG
    CTCAAAGGTA TAAAGCTGCG TGAAGCCAAT CAACAGCAGC AATTTAATCG CAATGTAGAA
    GACATTGAGT TGTGGCTGTA TGAAGTGGAG GGTCACTTGG CTTCTGATGA TTATGGAAAA
    GATCTTACCA GCGTTCAGAA TCTTCAGAAG AAACATGCCC TGCTAGAGGC AGATGTTGCT
    GCCCATCAGG ATCGGATAGA TGGCATTACC ATCCAGGCAC GCCAGTTCCA AGATGCTGGG
    CACTTTGATG CTGACAATAT CAAGAAGAAA CAAGAAGCTT TAGTAGCTCG TTATGAAGCT
    CTGAAGGATC CTATGGTGGC TCGCAAGCAG AAACTTGCAG ATTCTCTTCG CCTGCAGCAG
    CTTTTCCGTG ACATCGAGGA TGAAGAGACC TGGATCAGGG AAAAGGAACC TATTGCAGCC
    TCAACAAACC GAGGCAAGGA CTTAATTGGT GTCCAGAATC TGCTAAAGAA GCACCAGGCT
    TTGCAGGCAG AAATTGCAGG CCATGAGCCT CGCATTAAAG CAGTCACACA AAAAGGAAAT
    GCGATGGTGG AAGAGGGACA TTTTGCTGCT GAAGATGTGA AGATCAAATT GAATGAACTA
    AACCAAAAAT GGGACTCTCT GAAAGCCAAG GCATCTCAAC GGCGACAGGA TCTAGAGGAT
    TCTCTGCAAG CTCAGCAGTA TTTTGCTGAT GCTAATGAGG CAGAATCATG GATGAGGGAA
    AAGGAGCCCA TTGTAGGCAG CACGGATTAT GGAAAGGATG AAGACTCTGC TGAGGCTCTC
    CTGAAGAAGC ATGAAGCTTT GATGTCTGAT CTTTCTGCTT ACGGCAGTAG CATACAGGCA
    TTAAGGGAAC AGGCCCAGTC CTGCAGGCAA CAAGTTGCTC CCACTGATGA TGAAACTGGA
    AAAGAGCTTG TGCTAGCACT CTATGATTAC CAAGAGAAGA GTCCTCGGGA GGTGACTATG
    AAGAAAGGAG ATATTCTAAC CCTGCTCAAC AGCACCAACA AGGACTGGTG GAAGGTTGAA
    GTTAACGATC GTCAGGGCTT TGTACCAGCT GCCTATGTGA AAAAACTAGA TCCTGCCCAG
    TCTGCATCCC GAGAGAATCT TCTAGAAGAG CAAGGCAGCA TAGCATTGCG ACAGGAGCAA
    ATTGACAACC AGTATCATTC CCTTCTTGAG CTGGGAGAAA AACGTAAAGG CATGCTAGAA
    AAAAGCTGCA AGAAGTTTAT GCTTTTCCGT GAGGCTAATG AGCTTCAACA GTGGATCAAT
    GAGAAGGAAG CTGCGCTCAC TAATGAGGAA GTGGGTGCTG ATTTGGAGCA GGTGGAGGTG
    CTACAGAAGA AGTTTGATGA TTTTCAGAAG GATTTAAAAG CTAATGAGTC ACGACTGAAG
    GACATAAACA AGGTTGCAAA TGACCTGGAG TCTGAAGGGC TGATGGCAGA GGAAGTGCAA
    GCAGTAGAGC ACCAGGAAGT CTATGGAATG ATGCCCAGAG ATGAAACTGA TTCTAAGACA
    GCCTCTCCTT GGAAGTCTGC ACGTATGATG GTACACACAG TGGCAACGTT TAACTCAATC
    AAGGAACTGA ATGAACGCTG GAGATCCCTG CAGCAGTTGG CAGAGGAGCG AAGCCAGCTG
    TTGGGCAGCG CTCATGAAGT CCAGAGGTTC CACAGAGATG CTGATGAAAC AAAAGAATGG
    ATAGAAGAGA AGAATCAAGC ATTAAATACC GACAACTATG GACATGACTT GGCCAGTGTG
    CAGGCTCTGC AGCGCAAGCA TGAAGGCTTT GAGAGAGACT TGGCAGCTCT TGGAGACAAG
    GTGAATTCTC TTGGTGAAAC TGCCCAGCGT CTGATCCAGT CACATCCAGA ATCAGCTGAA
    GATCTTCAAG AAAAATGTAC TGAGTTGAAC CAAGCCTGGA GTAGTCTGGG AAAACGTGCT
    GACCAACGCA AAGAGAAGCT TGGAGATTCT CATGACCTGC AGCGTTTCCT CAGTGATTTT
    AGAGACCTTA TGTCTTGGAT CAATGGAATC CGGGGTCTGG TCTCCTCAGA TGAACTTGCA
    AAAGATGTGA CTGGAGCTGA AGCTCTGTTG GAAAGGCATC AGGAGCACCG TACTGAAATA
    GATGCACGGG CTGGCACTTT TCAGGCATTT GAACAGTTTG GACAGCAGCT TCTGGCTCGT
    GGACACTATG CCAGCCCAGA GATTAAGGAG AAACTGGATA TTCTAGATCA AGAACGGACA
    GACCTAGAGA AGGCCTGGGT CCAGCGCAGA ATGATGCTAG ACCAGTGCTT AGAACTACAG
    CTGTTTCATC GGGATTGTGA ACAAGCTGAA AACTGGATGG CTGCCCGAGA GGCGTTCCTA
    AATACAGAAG ACAAAGGAGA CTCCTTAGAC AGCGTGGAGG CACTCATCAA GAAGCATGAA
    GATTTCGATA AAGCAATCAA TGTCCAGGAA GAGAAAATTG CTGTCTTGCA GTCTTTTGCT
    GACCAACTGA TTGCTGCTGA TCATTATGCA AAAGGAGTCA TTGCTAACAG ACGCAATGAG
    GTTCTGGACA GGTGGCGTCG TCTGAAGGCT CAGATGATTG AGAAGAGATC TAAGCTGGGA
    GAATCTCAGA CCCTCCAACA GTTCAGTCGT GATGTTGATG AAATAGAAGC TTGGATCAGT
    GAAAAGCTTC AAACTGCAAG TGATGAGTCA TATAAGGATC CCACAAACAT CCAGCTTTCC
    AAACTGCTGA GCAAGCACCA GAAGCACCAA GCCTTTGAAG CTGAGCTCCA CGCCAACGCA
    GATCGGATTC GTGGAGTCAT TGAAATGGGG AACTCTCTTA TTGAAAGAGG AGCGTGTGCT
    GGCAGCGAGG ATGCTGTGAA GGCTCGGCTG GCTGCCCTGG CTGACCAATG GGAGTTCCTG
    GTCCAGAAGT CATCAGAGAA GAGTCAGAAA CTGAAAGAAG CAAATAAACA GCAGAATTTC
    AATACCGGCA TCAAGGACTT TGATTTCTGG CTTTCAGAGG TGGAAGCTTT GTTGGCATCT
    GAAGACTATG GGAAGGACTT GGCATCAGTG AACAACCTTC TGAAGAAGCA CCAATTACTG
    GAAGCTGATA TATCTGCTCA TGAGGATCGG CTGAAGGACC TGAACAGCCA GGCTGACAGT
    TTGATGACCA GCAGTGCTTT CGATACCTCC CAAGTAAAGG ATAAACGTGA AACTATAAAT
    GGGCGCTTCC AGAGAATCAA GAGCATGGCA GCTGCCCGCC GTGCGAAGCT CAACGAGTCG
    CACCGCTTGC ATCAGTTCTT CCGTGACATG GATGATGAGG AGTCCTGGAT CAAAGAGAAG
    AAACTGTTGG TGAGCTCAGA GGACTATGGC AGAGACCTGA CTGGTGTGCA GAACCTGAGG
    AAGAAACATA AGCGCTTGGA AGCAGAATTA GCTGCCCATG AACCTGCTAT CCAGGGTGTT
    CTAGACACTG GCAAGAAGCT TTCAGATGAT AACACAATTG GGAAGGAGGA GATACAGCAA
    AGACTGGCTC AGTTTGTGGA CCACTGGAAA GAGTTAAAAC AGCTGGCAGC TGCTCGGGGG
    CAGCGTCTGG AGGAGTCCCT GGAGTACCAA CAGTTTGTAG CAAATGTTGA GGAAGAGGAG
    GCATGGATCA ACGAGAAAAT GACATTGGTA GCCAGTGAGG ATTATGGAGA CACACTTGCT
    GCTATCCAGG GCTTGCTGAA GAAGCATGAA GCATTCGAGA CTGACTTTAC TGTCCACAAA
    GACAGAGTGA ATGATGTTTG CGCTAATGGA GAGGATCTCA TTAAAAAGAA TAATCACCAT
    GTGGAGAACA TTACTGCTAA GATGAAGGGC CTCAAGGGCA AGGTATCAGA TCTGGAGAAA
    GCCGCAGCTC AGAGGAAAGC CAAACTGGAT GAGAACTCTG CCTTCCTCCA GTTCAACTGG
    AAAGCAGATG TGGTGGAGTC ATGGATAGGT GAGAAGGAAA ACAGTCTGAA GACAGATGAT
    TATGGCCGTG ACCTCTCCTC TGTGCAAACA CTACTCACCA AACAGGAAAC CTTTGATGCT
    GGACTTCAGG CTTTCCAGCA GGAGGGAATT GCAAACATCA CTGCTCTGAA AGACCAGCTA
    CTGGCAGCCA AACATATCCA ATCAAAGGCC ATTGAGGTTC GTCACGCTTC CTTGATGAAA
    CGCTGGAATC AGCTGCTTGC TAATTCTGCA GCCAGGAAAA AGAAACTCTT GGAGGCTCAG
    GAGCACTTCA GAAAGGTTGA AGATCTCTTC TTGACTTTTG CCAAGAAGGC CTCTGCCTTC
    AACAGTTGGT TTGAGAATGC TGAGGAGGAC CTGACGGATC CCGTGCGCTG CAATTCCCTG
    GAAGAAATCA AAGCACTGCG AGAAGCCCAC GATGCGTTCC GTTCCTCACT TAGCTCTGCC
    CAAGCTGACT TCAACCAGCT GGCAGAGCTT GATCGGCAGA TCAAGAGCTT CCGTGTAGCC
    TCCAACCCGT ACACTTGGTT TACTATGGAG GCTCTTGAAG AAACTTGGAG GAATCTGCAG
    AAAATTATCA AGGAGCGTGA ATTGGAGTTG CAGAAGGAGC AGCGGAGGCA GGAAGAAAAT
    GACAAGTTGC GCCAGGAGTT TGCTCAGCAT GCTAATGCCT TCCACCAGTG GATTCAGGAG
    ACAAGGACTT ACCTGCTAGA TGGCATAGCC TATCGTCGTG TCATTCGTGT CTATCAGTAT
    GAAGTTGGGG ATGATCTATC TGGAAGGTCC TGCATGGTGG AGGAGTCGGG AACACTGGAA
    TCCCAGCTGG AAGCTACTAA ACGCAAGCAC CAGGAGATCC GGGCTATGAG GAGCCAGCTG
    AAGAAGATTG AGGACCTTGG AGCAGCCATG GAAGAGGCAC TTATCTTGGA CAACAAATAC
    ACAGAGCACA GCACCGTGGG GCTGGCGCAG CAGTGGGACC AGCTTGACCA GCTGGGAATG
    AGAATGCAGC ACAATCTGGA ACAGCAGATT CAAGCTCGAA ACACAACTGG AGTCACAGAG
    GAGGCCCTGA AGGAGTTCAG CATGATGTTC AAGCACTTTG ACAAGGACAA ATCTGGACGT
    CTTAATCACC AGGAGTTTAA GTCTTGCTTG CGCTCCCTTG GCTATGACCT GCCCATGGTT
    GAGGAAGGAG AGCCTGACCC CGAATTTGAG TCTATTCTTG ACACTGTTGA TCCCAACAGG
    GATGGCCACG TCTCGCTGCA GGAGTACATG GCGTTCATGA TCAGCAGGGA AACAGAGAAC
    GTGAAATCCA GCGAGGAGAT TGAGAGTGCT TTCCGTGCCC TCAGCTCGGA GGGGAAGCCT
    TACGTGACCA AGGAGGAGCT CTACCAGAAC CTGACCCGGG AGCAGGCCGA CTACTGCATT
    TCTCACATGA AGCCCTACAT GGATGGCAAG GGCAGAGAAC TTCCTTCTGC CTACGACTAC
    ATAGAATTTA CACGTTCACT CTTTGTGAAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015134776.1
    CDS14..7486
    Translation

    Target ORF information:

    RefSeq Version XM_015279290.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015279290.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
    TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG
    TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC
    TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT
    GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT
    GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG
    ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAA CTCGTCTACA GGAGCTGCAC
    CGACTATGGG AGTTACTGCT GGAAAAAATG AGAGAGAAGG GAGTGAAGCT GTTGCAAGCA
    CAGAAGCTGG TGCAATTCTT ACGGGAATGT GAAGATGTCA TGGACTGGAT CAATGACAAG
    GAAGCAATAG TGACCTCAGA AGAGCTTGGA CAGGACTTAG AGCATGTTGA GGTTTTGCAG
    AAGAAATTTG AAGAGTTCCA AACAGATCTG GCAGCTCATG AGGAGAGAGT GAATGAAGTG
    AACCAGTTCG CTGGCAAACT TATCCAGGAA CAGCACCCTG AGGAGGAACT TATAAAGTCC
    AAACAGGATG AAGTAAATGC AAGCTGGCAG CGTCTTAAGG GGCTTGCCCA ACAGAGGCAA
    GGAAAACTCT TTGGGGCAGC TGAAGTTCAG CGCTTCAACA GGGATGTGGA TGAAACTATC
    AGCTGGATTA AGGAGAAAGG GCAGTTGATG GCCTCAGATG ACTTTGGCAG AGACTTGGCC
    AGCGTGCAAG CTTTACTACG TAAGCATGAA GGCCTGGAAA GAGATCTTGC AGCTTTGGAG
    GATAAGGTTA AGGCCCTCTG TGCAGAAGCT GACCGTTTGC AGCAGTCTCA CCCTATAAAT
    GCTTCTCAAA TTCAAGTGAA ACGGGAGGAA CTGATTGCCA ACTGGGAGCA GATCCGAACT
    CTGGCAGCAG AGAGGCATGC TCGCCTTAAT GACTCCTACA GGTTGCAGCG CTTTCTTGCA
    GATTTCCGGG ACCTCACTAG CTGGGTAACT GAGATGAAGG CTCTGATAAA TGCTGATGAG
    CTTGCCAATG ATGTGGCTGG AGCAGAAGCC CTTCTAGATA GACATCAGGA ACATAAGGGA
    GAAATTGATG CTCATGAGGA TAGCTTCAGA TCTGCTGATG AGTCTGGGCA GGCTTTGCTT
    GCAGCTGGGC ACTATGCTTC TGATGAAGTT AAAGAAAAGC TGACTATCCT CTCAGATGAA
    AGATCAGCCT TGCTGGAACT ATGGGAGCTT CGCAGGCAAC AGTATGAGCA GTGCATGGAT
    CTGCAGCTTT TCTACAGAGA TACTGAACAA GTTGACAACT GGATGAGCAA ACAAGAAGCT
    TTTCTGCTGA ACGAAGACCT TGGTGATTCT CTGGATAGCG TGGAGGCTCT TCTAAAGAAG
    CATGAAGATT TTGAGAAATC CCTAAGTGCT CAAGAGGAGA AGATCACAGC ACTGGATGAG
    TTTGCTACTA AACTGATTCA AAATAACCAT TATGCCATGG ATGACGTTGC TACGCGCAGA
    GATGCTCTTC TGAGTCGCCG AAATGCTCTT CATGAAAGAG CCATGAAGCG CCGTGCCCAA
    CTGGCAGACT CTTTCCATCT CCAGCAGTTT TTCAGGGACT CTGATGAACT GAAGAGTTGG
    GTTAATGAAA AAATGAAAAC TGCTACCGAT GAGGCTTACA AGGATCCATC CAACTTGCAA
    GGTAAAGTTC AGAAGCATCA GGCTTTTGAA GCAGAGCTTT CTGCTAATCA GAGTCGTATT
    GATGCACTGG AGAAAGCTGG CCAGAAGTTG ATTGATGTCA ATCACTATGC ATCTGATGAG
    GTGGCAGCTC GCATGAATGA AGTCATCAGC TTGTGGAAGA AACTTCTGGA AGCCACCGAG
    CTCAAAGGTA TAAAGCTGCG TGAAGCCAAT CAACAGCAGC AATTTAATCG CAATGTAGAA
    GACATTGAGT TGTGGCTGTA TGAAGTGGAG GGTCACTTGG CTTCTGATGA TTATGGAAAA
    GATCTTACCA GCGTTCAGAA TCTTCAGAAG AAACATGCCC TGCTAGAGGC AGATGTTGCT
    GCCCATCAGG ATCGGATAGA TGGCATTACC ATCCAGGCAC GCCAGTTCCA AGATGCTGGG
    CACTTTGATG CTGACAATAT CAAGAAGAAA CAAGAAGCTT TAGTAGCTCG TTATGAAGCT
    CTGAAGGATC CTATGGTGGC TCGCAAGCAG AAACTTGCAG ATTCTCTTCG CCTGCAGCAG
    CTTTTCCGTG ACATCGAGGA TGAAGAGACC TGGATCAGGG AAAAGGAACC TATTGCAGCC
    TCAACAAACC GAGGCAAGGA CTTAATTGGT GTCCAGAATC TGCTAAAGAA GCACCAGGCT
    TTGCAGGCAG AAATTGCAGG CCATGAGCCT CGCATTAAAG CAGTCACACA AAAAGGAAAT
    GCGATGGTGG AAGAGGGACA TTTTGCTGCT GAAGATGTGA AGATCAAATT GAATGAACTA
    AACCAAAAAT GGGACTCTCT GAAAGCCAAG GCATCTCAAC GGCGACAGGA TCTAGAGGAT
    TCTCTGCAAG CTCAGCAGTA TTTTGCTGAT GCTAATGAGG CAGAATCATG GATGAGGGAA
    AAGGAGCCCA TTGTAGGCAG CACGGATTAT GGAAAGGATG AAGACTCTGC TGAGGCTCTC
    CTGAAGAAGC ATGAAGCTTT GATGTCTGAT CTTTCTGCTT ACGGCAGTAG CATACAGGCA
    TTAAGGGAAC AGGCCCAGTC CTGCAGGCAA CAAGTTGCTC CCACTGATGA TGAAACTGGA
    AAAGAGCTTG TGCTAGCACT CTATGATTAC CAAGAGAAGA GTCCTCGGGA GGTGACTATG
    AAGAAAGGAG ATATTCTAAC CCTGCTCAAC AGCACCAACA AGGACTGGTG GAAGGTTGAA
    GTTAACGATC GTCAGGGCTT TGTACCAGCT GCCTATGTGA AAAAACTAGA TCCTGCCCAG
    TCTGCATCCC GAGAGAATCT TCTAGAAGAG CAAGGCAGCA TAGCATTGCG ACAGGAGCAA
    ATTGACAACC AGTATCATTC CCTTCTTGAG CTGGGAGAAA AACGTAAAGG CATGCTAGAA
    AAAAGCTGCA AGAAGTTTAT GCTTTTCCGT GAGGCTAATG AGCTTCAACA GTGGATCAAT
    GAGAAGGAAG CTGCGCTCAC TAATGAGGAA GTGGGTGCTG ATTTGGAGCA GGTGGAGGTG
    CTACAGAAGA AGTTTGATGA TTTTCAGAAG GATTTAAAAG CTAATGAGTC ACGACTGAAG
    GACATAAACA AGGTTGCAAA TGACCTGGAG TCTGAAGGGC TGATGGCAGA GGAAGTGCAA
    GCAGTAGAGC ACCAGGAAGT CTATGGAATG ATGCCCAGAG ATGAAACTGA TTCTAAGACA
    GCCTCTCCTT GGAAGTCTGC ACGTATGATG GTACACACAG TGGCAACGTT TAACTCAATC
    AAGGAACTGA ATGAACGCTG GAGATCCCTG CAGCAGTTGG CAGAGGAGCG AAGCCAGCTG
    TTGGGCAGCG CTCATGAAGT CCAGAGGTTC CACAGAGATG CTGATGAAAC AAAAGAATGG
    ATAGAAGAGA AGAATCAAGC ATTAAATACC GACAACTATG GACATGACTT GGCCAGTGTG
    CAGGCTCTGC AGCGCAAGCA TGAAGGCTTT GAGAGAGACT TGGCAGCTCT TGGAGACAAG
    GTGAATTCTC TTGGTGAAAC TGCCCAGCGT CTGATCCAGT CACATCCAGA ATCAGCTGAA
    GATCTTCAAG AAAAATGTAC TGAGTTGAAC CAAGCCTGGA GTAGTCTGGG AAAACGTGCT
    GACCAACGCA AAGAGAAGCT TGGAGATTCT CATGACCTGC AGCGTTTCCT CAGTGATTTT
    AGAGACCTTA TGTCTTGGAT CAATGGAATC CGGGGTCTGG TCTCCTCAGA TGAACTTGCA
    AAAGATGTGA CTGGAGCTGA AGCTCTGTTG GAAAGGCATC AGGAGCACCG TACTGAAATA
    GATGCACGGG CTGGCACTTT TCAGGCATTT GAACAGTTTG GACAGCAGCT TCTGGCTCGT
    GGACACTATG CCAGCCCAGA GATTAAGGAG AAACTGGATA TTCTAGATCA AGAACGGACA
    GACCTAGAGA AGGCCTGGGT CCAGCGCAGA ATGATGCTAG ACCAGTGCTT AGAACTACAG
    CTGTTTCATC GGGATTGTGA ACAAGCTGAA AACTGGATGG CTGCCCGAGA GGCGTTCCTA
    AATACAGAAG ACAAAGGAGA CTCCTTAGAC AGCGTGGAGG CACTCATCAA GAAGCATGAA
    GATTTCGATA AAGCAATCAA TGTCCAGGAA GAGAAAATTG CTGTCTTGCA GTCTTTTGCT
    GACCAACTGA TTGCTGCTGA TCATTATGCA AAAGGAGTCA TTGCTAACAG ACGCAATGAG
    GTTCTGGACA GGTGGCGTCG TCTGAAGGCT CAGATGATTG AGAAGAGATC TAAGCTGGGA
    GAATCTCAGA CCCTCCAACA GTTCAGTCGT GATGTTGATG AAATAGAAGC TTGGATCAGT
    GAAAAGCTTC AAACTGCAAG TGATGAGTCA TATAAGGATC CCACAAACAT CCAGCTTTCC
    AAACTGCTGA GCAAGCACCA GAAGCACCAA GCCTTTGAAG CTGAGCTCCA CGCCAACGCA
    GATCGGATTC GTGGAGTCAT TGAAATGGGG AACTCTCTTA TTGAAAGAGG AGCGTGTGCT
    GGCAGCGAGG ATGCTGTGAA GGCTCGGCTG GCTGCCCTGG CTGACCAATG GGAGTTCCTG
    GTCCAGAAGT CATCAGAGAA GAGTCAGAAA CTGAAAGAAG CAAATAAACA GCAGAATTTC
    AATACCGGCA TCAAGGACTT TGATTTCTGG CTTTCAGAGG TGGAAGCTTT GTTGGCATCT
    GAAGACTATG GGAAGGACTT GGCATCAGTG AACAACCTTC TGAAGAAGCA CCAATTACTG
    GAAGCTGATA TATCTGCTCA TGAGGATCGG CTGAAGGACC TGAACAGCCA GGCTGACAGT
    TTGATGACCA GCAGTGCTTT CGATACCTCC CAAGTAAAGG ATAAACGTGA AACTATAAAT
    GGGCGCTTCC AGAGAATCAA GAGCATGGCA GCTGCCCGCC GTGCGAAGCT CAACGAGTCG
    CACCGCTTGC ATCAGTTCTT CCGTGACATG GATGATGAGG AGTCCTGGAT CAAAGAGAAG
    AAACTGTTGG TGAGCTCAGA GGACTATGGC AGAGACCTGA CTGGTGTGCA GAACCTGAGG
    AAGAAACATA AGCGCTTGGA AGCAGAATTA GCTGCCCATG AACCTGCTAT CCAGGGTGTT
    CTAGACACTG GCAAGAAGCT TTCAGATGAT AACACAATTG GGAAGGAGGA GATACAGCAA
    AGACTGGCTC AGTTTGTGGA CCACTGGAAA GAGTTAAAAC AGCTGGCAGC TGCTCGGGGG
    CAGCGTCTGG AGGAGTCCCT GGAGTACCAA CAGTTTGTAG CAAATGTTGA GGAAGAGGAG
    GCATGGATCA ACGAGAAAAT GACATTGGTA GCCAGTGAGG ATTATGGAGA CACACTTGCT
    GCTATCCAGG GCTTGCTGAA GAAGCATGAA GCATTCGAGA CTGACTTTAC TGTCCACAAA
    GACAGAGTGA ATGATGTTTG CGCTAATGGA GAGGATCTCA TTAAAAAGAA TAATCACCAT
    GTGGAGAACA TTACTGCTAA GATGAAGGGC CTCAAGGGCA AGGTATCAGA TCTGGAGAAA
    GCCGCAGCTC AGAGGAAAGC CAAACTGGAT GAGAACTCTG CCTTCCTCCA GTTCAACTGG
    AAAGCAGATG TGGTGGAGTC ATGGATAGGT GAGAAGGAAA ACAGTCTGAA GACAGATGAT
    TATGGCCGTG ACCTCTCCTC TGTGCAAACA CTACTCACCA AACAGGAAAC CTTTGATGCT
    GGACTTCAGG CTTTCCAGCA GGAGGGAATT GCAAACATCA CTGCTCTGAA AGACCAGCTA
    CTGGCAGCCA AACATATCCA ATCAAAGGCC ATTGAGGTTC GTCACGCTTC CTTGATGAAA
    CGCTGGAATC AGCTGCTTGC TAATTCTGCA GCCAGGAAAA AGAAACTCTT GGAGGCTCAG
    GAGCACTTCA GAAAGGTTGA AGATCTCTTC TTGACTTTTG CCAAGAAGGC CTCTGCCTTC
    AACAGTTGGT TTGAGAATGC TGAGGAGGAC CTGACGGATC CCGTGCGCTG CAATTCCCTG
    GAAGAAATCA AAGCACTGCG AGAAGCCCAC GATGCGTTCC GTTCCTCACT TAGCTCTGCC
    CAAGCTGACT TCAACCAGCT GGCAGAGCTT GATCGGCAGA TCAAGAGCTT CCGTGTAGCC
    TCCAACCCGT ACACTTGGTT TACTATGGAG GCTCTTGAAG AAACTTGGAG GAATCTGCAG
    AAAATTATCA AGGAGCGTGA ATTGGAGTTG CAGAAGGAGC AGCGGAGGCA GGAAGAAAAT
    GACAAGTTGC GCCAGGAGTT TGCTCAGCAT GCTAATGCCT TCCACCAGTG GATTCAGGAG
    ACAAGGACTT ACCTGCTAGA TGGCATAGCC TATCGTCGTG TCATTCGTGT CTATCAGTAT
    GAAGTTGGGG ATGATCTATC TGGAAGGTCC TGCATGGTGG AGGAGTCGGG AACACTGGAA
    TCCCAGCTGG AAGCTACTAA ACGCAAGCAC CAGGAGATCC GGGCTATGAG GAGCCAGCTG
    AAGAAGATTG AGGACCTTGG AGCAGCCATG GAAGAGGCAC TTATCTTGGA CAACAAATAC
    ACAGAGCACA GCACCGTGGG GCTGGCGCAG CAGTGGGACC AGCTTGACCA GCTGGGAATG
    AGAATGCAGC ACAATCTGGA ACAGCAGATT CAAGCTCGAA ACACAACTGG AGTCACAGAG
    GAGGCCCTGA AGGAGTTCAG CATGATGTTC AAGCACTTTG ACAAGGACAA ATCTGGACGT
    CTTAATCACC AGGAGTTTAA GTCTTGCTTG CGCTCCCTTG GCTATGACCT GCCCATGGTT
    GAGGAAGGAG AGCCTGACCC CGAATTTGAG TCTATTCTTG ACACTGTTGA TCCCAACAGG
    GATGGCCACG TCTCGCTGCA GGAGTACATG GCGTTCATGA TCAGCAGGGA AACAGAGAAC
    GTGAAATCCA GCGAGGAGAT TGAGAGTGCT TTCCGTGCCC TCAGCTCGGA GGGGAAGCCT
    TACGTGACCA AGGAGGAGCT CTACCAGAAC CTGACCCGGG AGCAGGCCGA CTACTGCATT
    TCTCACATGA AGCCCTACAT GGATGGCAAG GGCAGAGAAC TTCCTTCTGC CTACGACTAC
    ATAGAATTTA CACGTTCACT CTTTGTGAAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa43317
    Clone ID Related Accession (Same CDS sequence) XM_015279291.2 , XM_015279291.1
    Accession Version XM_015279291.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7470bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015279291.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
    TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG
    TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC
    TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT
    GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT
    GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG
    ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAA CTCGTCTACA GGAGCTGCAC
    CGACTATGGG AGTTACTGCT GGAAAAAATG AGAGAGAAGG GAGTGAAGCT GTTGCAAGCA
    CAGAAGCTGG TGCAATTCTT ACGGGAATGT GAAGATGTCA TGGACTGGAT CAATGACAAG
    GAAGCAATAG TGACCTCAGA AGAGCTTGGA CAGGACTTAG AGCATGTTGA GGTTTTGCAG
    AAGAAATTTG AAGAGTTCCA AACAGATCTG GCAGCTCATG AGGAGAGAGT GAATGAAGTG
    AACCAGTTCG CTGGCAAACT TATCCAGGAA CAGCACCCTG AGGAGGAACT TATAAAGTCC
    AAACAGGATG AAGTAAATGC AAGCTGGCAG CGTCTTAAGG GGCTTGCCCA ACAGAGGCAA
    GGAAAACTCT TTGGGGCAGC TGAAGTTCAG CGCTTCAACA GGGATGTGGA TGAAACTATC
    AGCTGGATTA AGGAGAAAGG GCAGTTGATG GCCTCAGATG ACTTTGGCAG AGACTTGGCC
    AGCGTGCAAG CTTTACTACG TAAGCATGAA GGCCTGGAAA GAGATCTTGC AGCTTTGGAG
    GATAAGGTTA AGGCCCTCTG TGCAGAAGCT GACCGTTTGC AGCAGTCTCA CCCTATAAAT
    GCTTCTCAAA TTCAAGTGAA ACGGGAGGAA CTGATTGCCA ACTGGGAGCA GATCCGAACT
    CTGGCAGCAG AGAGGCATGC TCGCCTTAAT GACTCCTACA GGTTGCAGCG CTTTCTTGCA
    GATTTCCGGG ACCTCACTAG CTGGGTAACT GAGATGAAGG CTCTGATAAA TGCTGATGAG
    CTTGCCAATG ATGTGGCTGG AGCAGAAGCC CTTCTAGATA GACATCAGGA ACATAAGGGA
    GAAATTGATG CTCATGAGGA TAGCTTCAGA TCTGCTGATG AGTCTGGGCA GGCTTTGCTT
    GCAGCTGGGC ACTATGCTTC TGATGAAGTT AAAGAAAAGC TGACTATCCT CTCAGATGAA
    AGATCAGCCT TGCTGGAACT ATGGGAGCTT CGCAGGCAAC AGTATGAGCA GTGCATGGAT
    CTGCAGCTTT TCTACAGAGA TACTGAACAA GTTGACAACT GGATGAGCAA ACAAGAAGCT
    TTTCTGCTGA ACGAAGACCT TGGTGATTCT CTGGATAGCG TGGAGGCTCT TCTAAAGAAG
    CATGAAGATT TTGAGAAATC CCTAAGTGCT CAAGAGGAGA AGATCACAGC ACTGGATGAG
    TTTGCTACTA AACTGATTCA AAATAACCAT TATGCCATGG ATGACGTTGC TACGCGCAGA
    GATGCTCTTC TGAGTCGCCG AAATGCTCTT CATGAAAGAG CCATGAAGCG CCGTGCCCAA
    CTGGCAGACT CTTTCCATCT CCAGCAGTTT TTCAGGGACT CTGATGAACT GAAGAGTTGG
    GTTAATGAAA AAATGAAAAC TGCTACCGAT GAGGCTTACA AGGATCCATC CAACTTGCAA
    GGTAAAGTTC AGAAGCATCA GGCTTTTGAA GCAGAGCTTT CTGCTAATCA GAGTCGTATT
    GATGCACTGG AGAAAGCTGG CCAGAAGTTG ATTGATGTCA ATCACTATGC ATCTGATGAG
    GTGGCAGCTC GCATGAATGA AGTCATCAGC TTGTGGAAGA AACTTCTGGA AGCCACCGAG
    CTCAAAGGTA TAAAGCTGCG TGAAGCCAAT CAACAGCAGC AATTTAATCG CAATGTAGAA
    GACATTGAGT TGTGGCTGTA TGAAGTGGAG GGTCACTTGG CTTCTGATGA TTATGGAAAA
    GATCTTACCA GCGTTCAGAA TCTTCAGAAG AAACATGCCC TGCTAGAGGC AGATGTTGCT
    GCCCATCAGG ATCGGATAGA TGGCATTACC ATCCAGGCAC GCCAGTTCCA AGATGCTGGG
    CACTTTGATG CTGACAATAT CAAGAAGAAA CAAGAAGCTT TAGTAGCTCG TTATGAAGCT
    CTGAAGGATC CTATGGTGGC TCGCAAGCAG AAACTTGCAG ATTCTCTTCG CCTGCAGCAG
    CTTTTCCGTG ACATCGAGGA TGAAGAGACC TGGATCAGGG AAAAGGAACC TATTGCAGCC
    TCAACAAACC GAGGCAAGGA CTTAATTGGT GTCCAGAATC TGCTAAAGAA GCACCAGGCT
    TTGCAGGCAG AAATTGCAGG CCATGAGCCT CGCATTAAAG CAGTCACACA AAAAGGAAAT
    GCGATGGTGG AAGAGGGACA TTTTGCTGCT GAAGATGTGA AGATCAAATT GAATGAACTA
    AACCAAAAAT GGGACTCTCT GAAAGCCAAG GCATCTCAAC GGCGACAGGA TCTAGAGGAT
    TCTCTGCAAG CTCAGCAGTA TTTTGCTGAT GCTAATGAGG CAGAATCATG GATGAGGGAA
    AAGGAGCCCA TTGTAGGCAG CACGGATTAT GGAAAGGATG AAGACTCTGC TGAGGCTCTC
    CTGAAGAAGC ATGAAGCTTT GATGTCTGAT CTTTCTGCTT ACGGCAGTAG CATACAGGCA
    TTAAGGGAAC AGGCCCAGTC CTGCAGGCAA CAAGTTGCTC CCACTGATGA TGAAACTGGA
    AAAGAGCTTG TGCTAGCACT CTATGATTAC CAAGAGAAGA GTCCTCGGGA GGTGACTATG
    AAGAAAGGAG ATATTCTAAC CCTGCTCAAC AGCACCAACA AGGACTGGTG GAAGGTTGAA
    GTTAACGATC GTCAGGGCTT TGTACCAGCT GCCTATGTGA AAAAACTAGA TCCTGCCCAG
    TCTGCATCCC GAGAGAATCT TCTAGAAGAG CAAGGCAGCA TAGCATTGCG ACAGGAGCAA
    ATTGACAACC AGACTCTCAT TACTAAGGAA GTCGGCAGTG TATCTCTGCG TATGAAACAG
    GTCGAAGAAC TGTATCATTC CCTTCTTGAG CTGGGAGAAA AACGTAAAGG CATGCTAGAA
    AAAAGCTGCA AGAAGTTTAT GCTTTTCCGT GAGGCTAATG AGCTTCAACA GTGGATCAAT
    GAGAAGGAAG CTGCGCTCAC TAATGAGGAA GTGGGTGCTG ATTTGGAGCA GGTGGAGGTG
    CTACAGAAGA AGTTTGATGA TTTTCAGAAG GATTTAAAAG CTAATGAGTC ACGACTGAAG
    GACATAAACA AGGTTGCAAA TGACCTGGAG TCTGAAGGGC TGATGGCAGA GGAAGTGCAA
    GCAGTAGAGC ACCAGGAAGT CTATGGAATG ATGCCCAGAG ATGAAACTGA TTCTAAGACA
    GCCTCTCCTT GGAAGTCTGC ACGTATGATG GTACACACAG TGGCAACGTT TAACTCAATC
    AAGGAACTGA ATGAACGCTG GAGATCCCTG CAGCAGTTGG CAGAGGAGCG AAGCCAGCTG
    TTGGGCAGCG CTCATGAAGT CCAGAGGTTC CACAGAGATG CTGATGAAAC AAAAGAATGG
    ATAGAAGAGA AGAATCAAGC ATTAAATACC GACAACTATG GACATGACTT GGCCAGTGTG
    CAGGCTCTGC AGCGCAAGCA TGAAGGCTTT GAGAGAGACT TGGCAGCTCT TGGAGACAAG
    GTGAATTCTC TTGGTGAAAC TGCCCAGCGT CTGATCCAGT CACATCCAGA ATCAGCTGAA
    GATCTTCAAG AAAAATGTAC TGAGTTGAAC CAAGCCTGGA GTAGTCTGGG AAAACGTGCT
    GACCAACGCA AAGAGAAGCT TGGAGATTCT CATGACCTGC AGCGTTTCCT CAGTGATTTT
    AGAGACCTTA TGTCTTGGAT CAATGGAATC CGGGGTCTGG TCTCCTCAGA TGAACTTGCA
    AAAGATGTGA CTGGAGCTGA AGCTCTGTTG GAAAGGCATC AGGAGCACCG TACTGAAATA
    GATGCACGGG CTGGCACTTT TCAGGCATTT GAACAGTTTG GACAGCAGCT TCTGGCTCGT
    GGACACTATG CCAGCCCAGA GATTAAGGAG AAACTGGATA TTCTAGATCA AGAACGGACA
    GACCTAGAGA AGGCCTGGGT CCAGCGCAGA ATGATGCTAG ACCAGTGCTT AGAACTACAG
    CTGTTTCATC GGGATTGTGA ACAAGCTGAA AACTGGATGG CTGCCCGAGA GGCGTTCCTA
    AATACAGAAG ACAAAGGAGA CTCCTTAGAC AGCGTGGAGG CACTCATCAA GAAGCATGAA
    GATTTCGATA AAGCAATCAA TGTCCAGGAA GAGAAAATTG CTGTCTTGCA GTCTTTTGCT
    GACCAACTGA TTGCTGCTGA TCATTATGCA AAAGGAGTCA TTGCTAACAG ACGCAATGAG
    GTTCTGGACA GGTGGCGTCG TCTGAAGGCT CAGATGATTG AGAAGAGATC TAAGCTGGGA
    GAATCTCAGA CCCTCCAACA GTTCAGTCGT GATGTTGATG AAATAGAAGC TTGGATCAGT
    GAAAAGCTTC AAACTGCAAG TGATGAGTCA TATAAGGATC CCACAAACAT CCAGCTTTCC
    AAACTGCTGA GCAAGCACCA GAAGCACCAA GCCTTTGAAG CTGAGCTCCA CGCCAACGCA
    GATCGGATTC GTGGAGTCAT TGAAATGGGG AACTCTCTTA TTGAAAGAGG AGCGTGTGCT
    GGCAGCGAGG ATGCTGTGAA GGCTCGGCTG GCTGCCCTGG CTGACCAATG GGAGTTCCTG
    GTCCAGAAGT CATCAGAGAA GAGTCAGAAA CTGAAAGAAG CAAATAAACA GCAGAATTTC
    AATACCGGCA TCAAGGACTT TGATTTCTGG CTTTCAGAGG TGGAAGCTTT GTTGGCATCT
    GAAGACTATG GGAAGGACTT GGCATCAGTG AACAACCTTC TGAAGAAGCA CCAATTACTG
    GAAGCTGATA TATCTGCTCA TGAGGATCGG CTGAAGGACC TGAACAGCCA GGCTGACAGT
    TTGATGACCA GCAGTGCTTT CGATACCTCC CAAGTAAAGG ATAAACGTGA AACTATAAAT
    GGGCGCTTCC AGAGAATCAA GAGCATGGCA GCTGCCCGCC GTGCGAAGCT CAACGAGTCG
    CACCGCTTGC ATCAGTTCTT CCGTGACATG GATGATGAGG AGTCCTGGAT CAAAGAGAAG
    AAACTGTTGG TGAGCTCAGA GGACTATGGC AGAGACCTGA CTGGTGTGCA GAACCTGAGG
    AAGAAACATA AGCGCTTGGA AGCAGAATTA GCTGCCCATG AACCTGCTAT CCAGGGTGTT
    CTAGACACTG GCAAGAAGCT TTCAGATGAT AACACAATTG GGAAGGAGGA GATACAGCAA
    AGACTGGCTC AGTTTGTGGA CCACTGGAAA GAGTTAAAAC AGCTGGCAGC TGCTCGGGGG
    CAGCGTCTGG AGGAGTCCCT GGAGTACCAA CAGTTTGTAG CAAATGTTGA GGAAGAGGAG
    GCATGGATCA ACGAGAAAAT GACATTGGTA GCCAGTGAGG ATTATGGAGA CACACTTGCT
    GCTATCCAGG GCTTGCTGAA GAAGCATGAA GCATTCGAGA CTGACTTTAC TGTCCACAAA
    GACAGAGTGA ATGATGTTTG CGCTAATGGA GAGGATCTCA TTAAAAAGAA TAATCACCAT
    GTGGAGAACA TTACTGCTAA GATGAAGGGC CTCAAGGGCA AGGTATCAGA TCTGGAGAAA
    GCCGCAGCTC AGAGGAAAGC CAAACTGGAT GAGAACTCTG CCTTCCTCCA GTTCAACTGG
    AAAGCAGATG TGGTGGAGTC ATGGATAGGT GAGAAGGAAA ACAGTCTGAA GACAGATGAT
    TATGGCCGTG ACCTCTCCTC TGTGCAAACA CTACTCACCA AACAGGAAAC CTTTGATGCT
    GGACTTCAGG CTTTCCAGCA GGAGGGAATT GCAAACATCA CTGCTCTGAA AGACCAGCTA
    CTGGCAGCCA AACATATCCA ATCAAAGGCC ATTGAGGTTC GTCACGCTTC CTTGATGAAA
    CGCTGGAATC AGCTGCTTGC TAATTCTGCA GCCAGGAAAA AGAAACTCTT GGAGGCTCAG
    GAGCACTTCA GAAAGGTTGA AGATCTCTTC TTGACTTTTG CCAAGAAGGC CTCTGCCTTC
    AACAGTTGGT TTGAGAATGC TGAGGAGGAC CTGACGGATC CCGTGCGCTG CAATTCCCTG
    GAAGAAATCA AAGCACTGCG AGAAGCCCAC GATGCGTTCC GTTCCTCACT TAGCTCTGCC
    CAAGCTGACT TCAACCAGCT GGCAGAGCTT GATCGGCAGA TCAAGAGCTT CCGTGTAGCC
    TCCAACCCGT ACACTTGGTT TACTATGGAG GCTCTTGAAG AAACTTGGAG GAATCTGCAG
    AAAATTATCA AGGAGCGTGA ATTGGAGTTG CAGAAGGAGC AGCGGAGGCA GGAAGAAAAT
    GACAAGTTGC GCCAGGAGTT TGCTCAGCAT GCTAATGCCT TCCACCAGTG GATTCAGGAG
    ACAAGGACTT ACCTGCTAGA TGGGTCCTGC ATGGTGGAGG AGTCGGGAAC ACTGGAATCC
    CAGCTGGAAG CTACTAAACG CAAGCACCAG GAGATCCGGG CTATGAGGAG CCAGCTGAAG
    AAGATTGAGG ACCTTGGAGC AGCCATGGAA GAGGCACTTA TCTTGGACAA CAAATACACA
    GAGCACAGCA CCGTGGGGCT GGCGCAGCAG TGGGACCAGC TTGACCAGCT GGGAATGAGA
    ATGCAGCACA ATCTGGAACA GCAGATTCAA GCTCGAAACA CAACTGGAGT CACAGAGGAG
    GCCCTGAAGG AGTTCAGCAT GATGTTCAAG CACTTTGACA AGGACAAATC TGGACGTCTT
    AATCACCAGG AGTTTAAGTC TTGCTTGCGC TCCCTTGGCT ATGACCTGCC CATGGTTGAG
    GAAGGAGAGC CTGACCCCGA ATTTGAGTCT ATTCTTGACA CTGTTGATCC CAACAGGGAT
    GGCCACGTCT CGCTGCAGGA GTACATGGCG TTCATGATCA GCAGGGAAAC AGAGAACGTG
    AAATCCAGCG AGGAGATTGA GAGTGCTTTC CGTGCCCTCA GCTCGGAGGG GAAGCCTTAC
    GTGACCAAGG AGGAGCTCTA CCAGAACCTG ACCCGGGAGC AGGCCGACTA CTGCATTTCT
    CACATGAAGC CCTACATGGA TGGCAAGGGC AGAGAACTTC CTTCTGCCTA CGACTACATA
    GAATTTACAC GTTCACTCTT TGTGAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015134777.1
    CDS9..7478
    Translation

    Target ORF information:

    RefSeq Version XM_015279291.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015279291.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
    TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG
    TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC
    TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT
    GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT
    GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG
    ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAA CTCGTCTACA GGAGCTGCAC
    CGACTATGGG AGTTACTGCT GGAAAAAATG AGAGAGAAGG GAGTGAAGCT GTTGCAAGCA
    CAGAAGCTGG TGCAATTCTT ACGGGAATGT GAAGATGTCA TGGACTGGAT CAATGACAAG
    GAAGCAATAG TGACCTCAGA AGAGCTTGGA CAGGACTTAG AGCATGTTGA GGTTTTGCAG
    AAGAAATTTG AAGAGTTCCA AACAGATCTG GCAGCTCATG AGGAGAGAGT GAATGAAGTG
    AACCAGTTCG CTGGCAAACT TATCCAGGAA CAGCACCCTG AGGAGGAACT TATAAAGTCC
    AAACAGGATG AAGTAAATGC AAGCTGGCAG CGTCTTAAGG GGCTTGCCCA ACAGAGGCAA
    GGAAAACTCT TTGGGGCAGC TGAAGTTCAG CGCTTCAACA GGGATGTGGA TGAAACTATC
    AGCTGGATTA AGGAGAAAGG GCAGTTGATG GCCTCAGATG ACTTTGGCAG AGACTTGGCC
    AGCGTGCAAG CTTTACTACG TAAGCATGAA GGCCTGGAAA GAGATCTTGC AGCTTTGGAG
    GATAAGGTTA AGGCCCTCTG TGCAGAAGCT GACCGTTTGC AGCAGTCTCA CCCTATAAAT
    GCTTCTCAAA TTCAAGTGAA ACGGGAGGAA CTGATTGCCA ACTGGGAGCA GATCCGAACT
    CTGGCAGCAG AGAGGCATGC TCGCCTTAAT GACTCCTACA GGTTGCAGCG CTTTCTTGCA
    GATTTCCGGG ACCTCACTAG CTGGGTAACT GAGATGAAGG CTCTGATAAA TGCTGATGAG
    CTTGCCAATG ATGTGGCTGG AGCAGAAGCC CTTCTAGATA GACATCAGGA ACATAAGGGA
    GAAATTGATG CTCATGAGGA TAGCTTCAGA TCTGCTGATG AGTCTGGGCA GGCTTTGCTT
    GCAGCTGGGC ACTATGCTTC TGATGAAGTT AAAGAAAAGC TGACTATCCT CTCAGATGAA
    AGATCAGCCT TGCTGGAACT ATGGGAGCTT CGCAGGCAAC AGTATGAGCA GTGCATGGAT
    CTGCAGCTTT TCTACAGAGA TACTGAACAA GTTGACAACT GGATGAGCAA ACAAGAAGCT
    TTTCTGCTGA ACGAAGACCT TGGTGATTCT CTGGATAGCG TGGAGGCTCT TCTAAAGAAG
    CATGAAGATT TTGAGAAATC CCTAAGTGCT CAAGAGGAGA AGATCACAGC ACTGGATGAG
    TTTGCTACTA AACTGATTCA AAATAACCAT TATGCCATGG ATGACGTTGC TACGCGCAGA
    GATGCTCTTC TGAGTCGCCG AAATGCTCTT CATGAAAGAG CCATGAAGCG CCGTGCCCAA
    CTGGCAGACT CTTTCCATCT CCAGCAGTTT TTCAGGGACT CTGATGAACT GAAGAGTTGG
    GTTAATGAAA AAATGAAAAC TGCTACCGAT GAGGCTTACA AGGATCCATC CAACTTGCAA
    GGTAAAGTTC AGAAGCATCA GGCTTTTGAA GCAGAGCTTT CTGCTAATCA GAGTCGTATT
    GATGCACTGG AGAAAGCTGG CCAGAAGTTG ATTGATGTCA ATCACTATGC ATCTGATGAG
    GTGGCAGCTC GCATGAATGA AGTCATCAGC TTGTGGAAGA AACTTCTGGA AGCCACCGAG
    CTCAAAGGTA TAAAGCTGCG TGAAGCCAAT CAACAGCAGC AATTTAATCG CAATGTAGAA
    GACATTGAGT TGTGGCTGTA TGAAGTGGAG GGTCACTTGG CTTCTGATGA TTATGGAAAA
    GATCTTACCA GCGTTCAGAA TCTTCAGAAG AAACATGCCC TGCTAGAGGC AGATGTTGCT
    GCCCATCAGG ATCGGATAGA TGGCATTACC ATCCAGGCAC GCCAGTTCCA AGATGCTGGG
    CACTTTGATG CTGACAATAT CAAGAAGAAA CAAGAAGCTT TAGTAGCTCG TTATGAAGCT
    CTGAAGGATC CTATGGTGGC TCGCAAGCAG AAACTTGCAG ATTCTCTTCG CCTGCAGCAG
    CTTTTCCGTG ACATCGAGGA TGAAGAGACC TGGATCAGGG AAAAGGAACC TATTGCAGCC
    TCAACAAACC GAGGCAAGGA CTTAATTGGT GTCCAGAATC TGCTAAAGAA GCACCAGGCT
    TTGCAGGCAG AAATTGCAGG CCATGAGCCT CGCATTAAAG CAGTCACACA AAAAGGAAAT
    GCGATGGTGG AAGAGGGACA TTTTGCTGCT GAAGATGTGA AGATCAAATT GAATGAACTA
    AACCAAAAAT GGGACTCTCT GAAAGCCAAG GCATCTCAAC GGCGACAGGA TCTAGAGGAT
    TCTCTGCAAG CTCAGCAGTA TTTTGCTGAT GCTAATGAGG CAGAATCATG GATGAGGGAA
    AAGGAGCCCA TTGTAGGCAG CACGGATTAT GGAAAGGATG AAGACTCTGC TGAGGCTCTC
    CTGAAGAAGC ATGAAGCTTT GATGTCTGAT CTTTCTGCTT ACGGCAGTAG CATACAGGCA
    TTAAGGGAAC AGGCCCAGTC CTGCAGGCAA CAAGTTGCTC CCACTGATGA TGAAACTGGA
    AAAGAGCTTG TGCTAGCACT CTATGATTAC CAAGAGAAGA GTCCTCGGGA GGTGACTATG
    AAGAAAGGAG ATATTCTAAC CCTGCTCAAC AGCACCAACA AGGACTGGTG GAAGGTTGAA
    GTTAACGATC GTCAGGGCTT TGTACCAGCT GCCTATGTGA AAAAACTAGA TCCTGCCCAG
    TCTGCATCCC GAGAGAATCT TCTAGAAGAG CAAGGCAGCA TAGCATTGCG ACAGGAGCAA
    ATTGACAACC AGACTCTCAT TACTAAGGAA GTCGGCAGTG TATCTCTGCG TATGAAACAG
    GTCGAAGAAC TGTATCATTC CCTTCTTGAG CTGGGAGAAA AACGTAAAGG CATGCTAGAA
    AAAAGCTGCA AGAAGTTTAT GCTTTTCCGT GAGGCTAATG AGCTTCAACA GTGGATCAAT
    GAGAAGGAAG CTGCGCTCAC TAATGAGGAA GTGGGTGCTG ATTTGGAGCA GGTGGAGGTG
    CTACAGAAGA AGTTTGATGA TTTTCAGAAG GATTTAAAAG CTAATGAGTC ACGACTGAAG
    GACATAAACA AGGTTGCAAA TGACCTGGAG TCTGAAGGGC TGATGGCAGA GGAAGTGCAA
    GCAGTAGAGC ACCAGGAAGT CTATGGAATG ATGCCCAGAG ATGAAACTGA TTCTAAGACA
    GCCTCTCCTT GGAAGTCTGC ACGTATGATG GTACACACAG TGGCAACGTT TAACTCAATC
    AAGGAACTGA ATGAACGCTG GAGATCCCTG CAGCAGTTGG CAGAGGAGCG AAGCCAGCTG
    TTGGGCAGCG CTCATGAAGT CCAGAGGTTC CACAGAGATG CTGATGAAAC AAAAGAATGG
    ATAGAAGAGA AGAATCAAGC ATTAAATACC GACAACTATG GACATGACTT GGCCAGTGTG
    CAGGCTCTGC AGCGCAAGCA TGAAGGCTTT GAGAGAGACT TGGCAGCTCT TGGAGACAAG
    GTGAATTCTC TTGGTGAAAC TGCCCAGCGT CTGATCCAGT CACATCCAGA ATCAGCTGAA
    GATCTTCAAG AAAAATGTAC TGAGTTGAAC CAAGCCTGGA GTAGTCTGGG AAAACGTGCT
    GACCAACGCA AAGAGAAGCT TGGAGATTCT CATGACCTGC AGCGTTTCCT CAGTGATTTT
    AGAGACCTTA TGTCTTGGAT CAATGGAATC CGGGGTCTGG TCTCCTCAGA TGAACTTGCA
    AAAGATGTGA CTGGAGCTGA AGCTCTGTTG GAAAGGCATC AGGAGCACCG TACTGAAATA
    GATGCACGGG CTGGCACTTT TCAGGCATTT GAACAGTTTG GACAGCAGCT TCTGGCTCGT
    GGACACTATG CCAGCCCAGA GATTAAGGAG AAACTGGATA TTCTAGATCA AGAACGGACA
    GACCTAGAGA AGGCCTGGGT CCAGCGCAGA ATGATGCTAG ACCAGTGCTT AGAACTACAG
    CTGTTTCATC GGGATTGTGA ACAAGCTGAA AACTGGATGG CTGCCCGAGA GGCGTTCCTA
    AATACAGAAG ACAAAGGAGA CTCCTTAGAC AGCGTGGAGG CACTCATCAA GAAGCATGAA
    GATTTCGATA AAGCAATCAA TGTCCAGGAA GAGAAAATTG CTGTCTTGCA GTCTTTTGCT
    GACCAACTGA TTGCTGCTGA TCATTATGCA AAAGGAGTCA TTGCTAACAG ACGCAATGAG
    GTTCTGGACA GGTGGCGTCG TCTGAAGGCT CAGATGATTG AGAAGAGATC TAAGCTGGGA
    GAATCTCAGA CCCTCCAACA GTTCAGTCGT GATGTTGATG AAATAGAAGC TTGGATCAGT
    GAAAAGCTTC AAACTGCAAG TGATGAGTCA TATAAGGATC CCACAAACAT CCAGCTTTCC
    AAACTGCTGA GCAAGCACCA GAAGCACCAA GCCTTTGAAG CTGAGCTCCA CGCCAACGCA
    GATCGGATTC GTGGAGTCAT TGAAATGGGG AACTCTCTTA TTGAAAGAGG AGCGTGTGCT
    GGCAGCGAGG ATGCTGTGAA GGCTCGGCTG GCTGCCCTGG CTGACCAATG GGAGTTCCTG
    GTCCAGAAGT CATCAGAGAA GAGTCAGAAA CTGAAAGAAG CAAATAAACA GCAGAATTTC
    AATACCGGCA TCAAGGACTT TGATTTCTGG CTTTCAGAGG TGGAAGCTTT GTTGGCATCT
    GAAGACTATG GGAAGGACTT GGCATCAGTG AACAACCTTC TGAAGAAGCA CCAATTACTG
    GAAGCTGATA TATCTGCTCA TGAGGATCGG CTGAAGGACC TGAACAGCCA GGCTGACAGT
    TTGATGACCA GCAGTGCTTT CGATACCTCC CAAGTAAAGG ATAAACGTGA AACTATAAAT
    GGGCGCTTCC AGAGAATCAA GAGCATGGCA GCTGCCCGCC GTGCGAAGCT CAACGAGTCG
    CACCGCTTGC ATCAGTTCTT CCGTGACATG GATGATGAGG AGTCCTGGAT CAAAGAGAAG
    AAACTGTTGG TGAGCTCAGA GGACTATGGC AGAGACCTGA CTGGTGTGCA GAACCTGAGG
    AAGAAACATA AGCGCTTGGA AGCAGAATTA GCTGCCCATG AACCTGCTAT CCAGGGTGTT
    CTAGACACTG GCAAGAAGCT TTCAGATGAT AACACAATTG GGAAGGAGGA GATACAGCAA
    AGACTGGCTC AGTTTGTGGA CCACTGGAAA GAGTTAAAAC AGCTGGCAGC TGCTCGGGGG
    CAGCGTCTGG AGGAGTCCCT GGAGTACCAA CAGTTTGTAG CAAATGTTGA GGAAGAGGAG
    GCATGGATCA ACGAGAAAAT GACATTGGTA GCCAGTGAGG ATTATGGAGA CACACTTGCT
    GCTATCCAGG GCTTGCTGAA GAAGCATGAA GCATTCGAGA CTGACTTTAC TGTCCACAAA
    GACAGAGTGA ATGATGTTTG CGCTAATGGA GAGGATCTCA TTAAAAAGAA TAATCACCAT
    GTGGAGAACA TTACTGCTAA GATGAAGGGC CTCAAGGGCA AGGTATCAGA TCTGGAGAAA
    GCCGCAGCTC AGAGGAAAGC CAAACTGGAT GAGAACTCTG CCTTCCTCCA GTTCAACTGG
    AAAGCAGATG TGGTGGAGTC ATGGATAGGT GAGAAGGAAA ACAGTCTGAA GACAGATGAT
    TATGGCCGTG ACCTCTCCTC TGTGCAAACA CTACTCACCA AACAGGAAAC CTTTGATGCT
    GGACTTCAGG CTTTCCAGCA GGAGGGAATT GCAAACATCA CTGCTCTGAA AGACCAGCTA
    CTGGCAGCCA AACATATCCA ATCAAAGGCC ATTGAGGTTC GTCACGCTTC CTTGATGAAA
    CGCTGGAATC AGCTGCTTGC TAATTCTGCA GCCAGGAAAA AGAAACTCTT GGAGGCTCAG
    GAGCACTTCA GAAAGGTTGA AGATCTCTTC TTGACTTTTG CCAAGAAGGC CTCTGCCTTC
    AACAGTTGGT TTGAGAATGC TGAGGAGGAC CTGACGGATC CCGTGCGCTG CAATTCCCTG
    GAAGAAATCA AAGCACTGCG AGAAGCCCAC GATGCGTTCC GTTCCTCACT TAGCTCTGCC
    CAAGCTGACT TCAACCAGCT GGCAGAGCTT GATCGGCAGA TCAAGAGCTT CCGTGTAGCC
    TCCAACCCGT ACACTTGGTT TACTATGGAG GCTCTTGAAG AAACTTGGAG GAATCTGCAG
    AAAATTATCA AGGAGCGTGA ATTGGAGTTG CAGAAGGAGC AGCGGAGGCA GGAAGAAAAT
    GACAAGTTGC GCCAGGAGTT TGCTCAGCAT GCTAATGCCT TCCACCAGTG GATTCAGGAG
    ACAAGGACTT ACCTGCTAGA TGGGTCCTGC ATGGTGGAGG AGTCGGGAAC ACTGGAATCC
    CAGCTGGAAG CTACTAAACG CAAGCACCAG GAGATCCGGG CTATGAGGAG CCAGCTGAAG
    AAGATTGAGG ACCTTGGAGC AGCCATGGAA GAGGCACTTA TCTTGGACAA CAAATACACA
    GAGCACAGCA CCGTGGGGCT GGCGCAGCAG TGGGACCAGC TTGACCAGCT GGGAATGAGA
    ATGCAGCACA ATCTGGAACA GCAGATTCAA GCTCGAAACA CAACTGGAGT CACAGAGGAG
    GCCCTGAAGG AGTTCAGCAT GATGTTCAAG CACTTTGACA AGGACAAATC TGGACGTCTT
    AATCACCAGG AGTTTAAGTC TTGCTTGCGC TCCCTTGGCT ATGACCTGCC CATGGTTGAG
    GAAGGAGAGC CTGACCCCGA ATTTGAGTCT ATTCTTGACA CTGTTGATCC CAACAGGGAT
    GGCCACGTCT CGCTGCAGGA GTACATGGCG TTCATGATCA GCAGGGAAAC AGAGAACGTG
    AAATCCAGCG AGGAGATTGA GAGTGCTTTC CGTGCCCTCA GCTCGGAGGG GAAGCCTTAC
    GTGACCAAGG AGGAGCTCTA CCAGAACCTG ACCCGGGAGC AGGCCGACTA CTGCATTTCT
    CACATGAAGC CCTACATGGA TGGCAAGGGC AGAGAACTTC CTTCTGCCTA CGACTACATA
    GAATTTACAC GTTCACTCTT TGTGAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa43317
    Clone ID Related Accession (Same CDS sequence) XM_015279291.2 , XM_015279291.1
    Accession Version XM_015279291.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7470bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
    TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG
    TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC
    TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT
    GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT
    GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG
    ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAA CTCGTCTACA GGAGCTGCAC
    CGACTATGGG AGTTACTGCT GGAAAAAATG AGAGAGAAGG GAGTGAAGCT GTTGCAAGCA
    CAGAAGCTGG TGCAATTCTT ACGGGAATGT GAAGATGTCA TGGACTGGAT CAATGACAAG
    GAAGCAATAG TGACCTCAGA AGAGCTTGGA CAGGACTTAG AGCATGTTGA GGTTTTGCAG
    AAGAAATTTG AAGAGTTCCA AACAGATCTG GCAGCTCATG AGGAGAGAGT GAATGAAGTG
    AACCAGTTCG CTGGCAAACT TATCCAGGAA CAGCACCCTG AGGAGGAACT TATAAAGTCC
    AAACAGGATG AAGTAAATGC AAGCTGGCAG CGTCTTAAGG GGCTTGCCCA ACAGAGGCAA
    GGAAAACTCT TTGGGGCAGC TGAAGTTCAG CGCTTCAACA GGGATGTGGA TGAAACTATC
    AGCTGGATTA AGGAGAAAGG GCAGTTGATG GCCTCAGATG ACTTTGGCAG AGACTTGGCC
    AGCGTGCAAG CTTTACTACG TAAGCATGAA GGCCTGGAAA GAGATCTTGC AGCTTTGGAG
    GATAAGGTTA AGGCCCTCTG TGCAGAAGCT GACCGTTTGC AGCAGTCTCA CCCTATAAAT
    GCTTCTCAAA TTCAAGTGAA ACGGGAGGAA CTGATTGCCA ACTGGGAGCA GATCCGAACT
    CTGGCAGCAG AGAGGCATGC TCGCCTTAAT GACTCCTACA GGTTGCAGCG CTTTCTTGCA
    GATTTCCGGG ACCTCACTAG CTGGGTAACT GAGATGAAGG CTCTGATAAA TGCTGATGAG
    CTTGCCAATG ATGTGGCTGG AGCAGAAGCC CTTCTAGATA GACATCAGGA ACATAAGGGA
    GAAATTGATG CTCATGAGGA TAGCTTCAGA TCTGCTGATG AGTCTGGGCA GGCTTTGCTT
    GCAGCTGGGC ACTATGCTTC TGATGAAGTT AAAGAAAAGC TGACTATCCT CTCAGATGAA
    AGATCAGCCT TGCTGGAACT ATGGGAGCTT CGCAGGCAAC AGTATGAGCA GTGCATGGAT
    CTGCAGCTTT TCTACAGAGA TACTGAACAA GTTGACAACT GGATGAGCAA ACAAGAAGCT
    TTTCTGCTGA ACGAAGACCT TGGTGATTCT CTGGATAGCG TGGAGGCTCT TCTAAAGAAG
    CATGAAGATT TTGAGAAATC CCTAAGTGCT CAAGAGGAGA AGATCACAGC ACTGGATGAG
    TTTGCTACTA AACTGATTCA AAATAACCAT TATGCCATGG ATGACGTTGC TACGCGCAGA
    GATGCTCTTC TGAGTCGCCG AAATGCTCTT CATGAAAGAG CCATGAAGCG CCGTGCCCAA
    CTGGCAGACT CTTTCCATCT CCAGCAGTTT TTCAGGGACT CTGATGAACT GAAGAGTTGG
    GTTAATGAAA AAATGAAAAC TGCTACCGAT GAGGCTTACA AGGATCCATC CAACTTGCAA
    GGTAAAGTTC AGAAGCATCA GGCTTTTGAA GCAGAGCTTT CTGCTAATCA GAGTCGTATT
    GATGCACTGG AGAAAGCTGG CCAGAAGTTG ATTGATGTCA ATCACTATGC ATCTGATGAG
    GTGGCAGCTC GCATGAATGA AGTCATCAGC TTGTGGAAGA AACTTCTGGA AGCCACCGAG
    CTCAAAGGTA TAAAGCTGCG TGAAGCCAAT CAACAGCAGC AATTTAATCG CAATGTAGAA
    GACATTGAGT TGTGGCTGTA TGAAGTGGAG GGTCACTTGG CTTCTGATGA TTATGGAAAA
    GATCTTACCA GCGTTCAGAA TCTTCAGAAG AAACATGCCC TGCTAGAGGC AGATGTTGCT
    GCCCATCAGG ATCGGATAGA TGGCATTACC ATCCAGGCAC GCCAGTTCCA AGATGCTGGG
    CACTTTGATG CTGACAATAT CAAGAAGAAA CAAGAAGCTT TAGTAGCTCG TTATGAAGCT
    CTGAAGGATC CTATGGTGGC TCGCAAGCAG AAACTTGCAG ATTCTCTTCG CCTGCAGCAG
    CTTTTCCGTG ACATCGAGGA TGAAGAGACC TGGATCAGGG AAAAGGAACC TATTGCAGCC
    TCAACAAACC GAGGCAAGGA CTTAATTGGT GTCCAGAATC TGCTAAAGAA GCACCAGGCT
    TTGCAGGCAG AAATTGCAGG CCATGAGCCT CGCATTAAAG CAGTCACACA AAAAGGAAAT
    GCGATGGTGG AAGAGGGACA TTTTGCTGCT GAAGATGTGA AGATCAAATT GAATGAACTA
    AACCAAAAAT GGGACTCTCT GAAAGCCAAG GCATCTCAAC GGCGACAGGA TCTAGAGGAT
    TCTCTGCAAG CTCAGCAGTA TTTTGCTGAT GCTAATGAGG CAGAATCATG GATGAGGGAA
    AAGGAGCCCA TTGTAGGCAG CACGGATTAT GGAAAGGATG AAGACTCTGC TGAGGCTCTC
    CTGAAGAAGC ATGAAGCTTT GATGTCTGAT CTTTCTGCTT ACGGCAGTAG CATACAGGCA
    TTAAGGGAAC AGGCCCAGTC CTGCAGGCAA CAAGTTGCTC CCACTGATGA TGAAACTGGA
    AAAGAGCTTG TGCTAGCACT CTATGATTAC CAAGAGAAGA GTCCTCGGGA GGTGACTATG
    AAGAAAGGAG ATATTCTAAC CCTGCTCAAC AGCACCAACA AGGACTGGTG GAAGGTTGAA
    GTTAACGATC GTCAGGGCTT TGTACCAGCT GCCTATGTGA AAAAACTAGA TCCTGCCCAG
    TCTGCATCCC GAGAGAATCT TCTAGAAGAG CAAGGCAGCA TAGCATTGCG ACAGGAGCAA
    ATTGACAACC AGACTCTCAT TACTAAGGAA GTCGGCAGTG TATCTCTGCG TATGAAACAG
    GTCGAAGAAC TGTATCATTC CCTTCTTGAG CTGGGAGAAA AACGTAAAGG CATGCTAGAA
    AAAAGCTGCA AGAAGTTTAT GCTTTTCCGT GAGGCTAATG AGCTTCAACA GTGGATCAAT
    GAGAAGGAAG CTGCGCTCAC TAATGAGGAA GTGGGTGCTG ATTTGGAGCA GGTGGAGGTG
    CTACAGAAGA AGTTTGATGA TTTTCAGAAG GATTTAAAAG CTAATGAGTC ACGACTGAAG
    GACATAAACA AGGTTGCAAA TGACCTGGAG TCTGAAGGGC TGATGGCAGA GGAAGTGCAA
    GCAGTAGAGC ACCAGGAAGT CTATGGAATG ATGCCCAGAG ATGAAACTGA TTCTAAGACA
    GCCTCTCCTT GGAAGTCTGC ACGTATGATG GTACACACAG TGGCAACGTT TAACTCAATC
    AAGGAACTGA ATGAACGCTG GAGATCCCTG CAGCAGTTGG CAGAGGAGCG AAGCCAGCTG
    TTGGGCAGCG CTCATGAAGT CCAGAGGTTC CACAGAGATG CTGATGAAAC AAAAGAATGG
    ATAGAAGAGA AGAATCAAGC ATTAAATACC GACAACTATG GACATGACTT GGCCAGTGTG
    CAGGCTCTGC AGCGCAAGCA TGAAGGCTTT GAGAGAGACT TGGCAGCTCT TGGAGACAAG
    GTGAATTCTC TTGGTGAAAC TGCCCAGCGT CTGATCCAGT CACATCCAGA ATCAGCTGAA
    GATCTTCAAG AAAAATGTAC TGAGTTGAAC CAAGCCTGGA GTAGTCTGGG AAAACGTGCT
    GACCAACGCA AAGAGAAGCT TGGAGATTCT CATGACCTGC AGCGTTTCCT CAGTGATTTT
    AGAGACCTTA TGTCTTGGAT CAATGGAATC CGGGGTCTGG TCTCCTCAGA TGAACTTGCA
    AAAGATGTGA CTGGAGCTGA AGCTCTGTTG GAAAGGCATC AGGAGCACCG TACTGAAATA
    GATGCACGGG CTGGCACTTT TCAGGCATTT GAACAGTTTG GACAGCAGCT TCTGGCTCGT
    GGACACTATG CCAGCCCAGA GATTAAGGAG AAACTGGATA TTCTAGATCA AGAACGGACA
    GACCTAGAGA AGGCCTGGGT CCAGCGCAGA ATGATGCTAG ACCAGTGCTT AGAACTACAG
    CTGTTTCATC GGGATTGTGA ACAAGCTGAA AACTGGATGG CTGCCCGAGA GGCGTTCCTA
    AATACAGAAG ACAAAGGAGA CTCCTTAGAC AGCGTGGAGG CACTCATCAA GAAGCATGAA
    GATTTCGATA AAGCAATCAA TGTCCAGGAA GAGAAAATTG CTGTCTTGCA GTCTTTTGCT
    GACCAACTGA TTGCTGCTGA TCATTATGCA AAAGGAGTCA TTGCTAACAG ACGCAATGAG
    GTTCTGGACA GGTGGCGTCG TCTGAAGGCT CAGATGATTG AGAAGAGATC TAAGCTGGGA
    GAATCTCAGA CCCTCCAACA GTTCAGTCGT GATGTTGATG AAATAGAAGC TTGGATCAGT
    GAAAAGCTTC AAACTGCAAG TGATGAGTCA TATAAGGATC CCACAAACAT CCAGCTTTCC
    AAACTGCTGA GCAAGCACCA GAAGCACCAA GCCTTTGAAG CTGAGCTCCA CGCCAACGCA
    GATCGGATTC GTGGAGTCAT TGAAATGGGG AACTCTCTTA TTGAAAGAGG AGCGTGTGCT
    GGCAGCGAGG ATGCTGTGAA GGCTCGGCTG GCTGCCCTGG CTGACCAATG GGAGTTCCTG
    GTCCAGAAGT CATCAGAGAA GAGTCAGAAA CTGAAAGAAG CAAATAAACA GCAGAATTTC
    AATACCGGCA TCAAGGACTT TGATTTCTGG CTTTCAGAGG TGGAAGCTTT GTTGGCATCT
    GAAGACTATG GGAAGGACTT GGCATCAGTG AACAACCTTC TGAAGAAGCA CCAATTACTG
    GAAGCTGATA TATCTGCTCA TGAGGATCGG CTGAAGGACC TGAACAGCCA GGCTGACAGT
    TTGATGACCA GCAGTGCTTT CGATACCTCC CAAGTAAAGG ATAAACGTGA AACTATAAAT
    GGGCGCTTCC AGAGAATCAA GAGCATGGCA GCTGCCCGCC GTGCGAAGCT CAACGAGTCG
    CACCGCTTGC ATCAGTTCTT CCGTGACATG GATGATGAGG AGTCCTGGAT CAAAGAGAAG
    AAACTGTTGG TGAGCTCAGA GGACTATGGC AGAGACCTGA CTGGTGTGCA GAACCTGAGG
    AAGAAACATA AGCGCTTGGA AGCAGAATTA GCTGCCCATG AACCTGCTAT CCAGGGTGTT
    CTAGACACTG GCAAGAAGCT TTCAGATGAT AACACAATTG GGAAGGAGGA GATACAGCAA
    AGACTGGCTC AGTTTGTGGA CCACTGGAAA GAGTTAAAAC AGCTGGCAGC TGCTCGGGGG
    CAGCGTCTGG AGGAGTCCCT GGAGTACCAA CAGTTTGTAG CAAATGTTGA GGAAGAGGAG
    GCATGGATCA ACGAGAAAAT GACATTGGTA GCCAGTGAGG ATTATGGAGA CACACTTGCT
    GCTATCCAGG GCTTGCTGAA GAAGCATGAA GCATTCGAGA CTGACTTTAC TGTCCACAAA
    GACAGAGTGA ATGATGTTTG CGCTAATGGA GAGGATCTCA TTAAAAAGAA TAATCACCAT
    GTGGAGAACA TTACTGCTAA GATGAAGGGC CTCAAGGGCA AGGTATCAGA TCTGGAGAAA
    GCCGCAGCTC AGAGGAAAGC CAAACTGGAT GAGAACTCTG CCTTCCTCCA GTTCAACTGG
    AAAGCAGATG TGGTGGAGTC ATGGATAGGT GAGAAGGAAA ACAGTCTGAA GACAGATGAT
    TATGGCCGTG ACCTCTCCTC TGTGCAAACA CTACTCACCA AACAGGAAAC CTTTGATGCT
    GGACTTCAGG CTTTCCAGCA GGAGGGAATT GCAAACATCA CTGCTCTGAA AGACCAGCTA
    CTGGCAGCCA AACATATCCA ATCAAAGGCC ATTGAGGTTC GTCACGCTTC CTTGATGAAA
    CGCTGGAATC AGCTGCTTGC TAATTCTGCA GCCAGGAAAA AGAAACTCTT GGAGGCTCAG
    GAGCACTTCA GAAAGGTTGA AGATCTCTTC TTGACTTTTG CCAAGAAGGC CTCTGCCTTC
    AACAGTTGGT TTGAGAATGC TGAGGAGGAC CTGACGGATC CCGTGCGCTG CAATTCCCTG
    GAAGAAATCA AAGCACTGCG AGAAGCCCAC GATGCGTTCC GTTCCTCACT TAGCTCTGCC
    CAAGCTGACT TCAACCAGCT GGCAGAGCTT GATCGGCAGA TCAAGAGCTT CCGTGTAGCC
    TCCAACCCGT ACACTTGGTT TACTATGGAG GCTCTTGAAG AAACTTGGAG GAATCTGCAG
    AAAATTATCA AGGAGCGTGA ATTGGAGTTG CAGAAGGAGC AGCGGAGGCA GGAAGAAAAT
    GACAAGTTGC GCCAGGAGTT TGCTCAGCAT GCTAATGCCT TCCACCAGTG GATTCAGGAG
    ACAAGGACTT ACCTGCTAGA TGGGTCCTGC ATGGTGGAGG AGTCGGGAAC ACTGGAATCC
    CAGCTGGAAG CTACTAAACG CAAGCACCAG GAGATCCGGG CTATGAGGAG CCAGCTGAAG
    AAGATTGAGG ACCTTGGAGC AGCCATGGAA GAGGCACTTA TCTTGGACAA CAAATACACA
    GAGCACAGCA CCGTGGGGCT GGCGCAGCAG TGGGACCAGC TTGACCAGCT GGGAATGAGA
    ATGCAGCACA ATCTGGAACA GCAGATTCAA GCTCGAAACA CAACTGGAGT CACAGAGGAG
    GCCCTGAAGG AGTTCAGCAT GATGTTCAAG CACTTTGACA AGGACAAATC TGGACGTCTT
    AATCACCAGG AGTTTAAGTC TTGCTTGCGC TCCCTTGGCT ATGACCTGCC CATGGTTGAG
    GAAGGAGAGC CTGACCCCGA ATTTGAGTCT ATTCTTGACA CTGTTGATCC CAACAGGGAT
    GGCCACGTCT CGCTGCAGGA GTACATGGCG TTCATGATCA GCAGGGAAAC AGAGAACGTG
    AAATCCAGCG AGGAGATTGA GAGTGCTTTC CGTGCCCTCA GCTCGGAGGG GAAGCCTTAC
    GTGACCAAGG AGGAGCTCTA CCAGAACCTG ACCCGGGAGC AGGCCGACTA CTGCATTTCT
    CACATGAAGC CCTACATGGA TGGCAAGGGC AGAGAACTTC CTTCTGCCTA CGACTACATA
    GAATTTACAC GTTCACTCTT TGTGAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015134777.1
    CDS10..7479
    Translation

    Target ORF information:

    RefSeq Version XM_015279291.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015279291.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
    TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG
    TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC
    TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT
    GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT
    GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG
    ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAA CTCGTCTACA GGAGCTGCAC
    CGACTATGGG AGTTACTGCT GGAAAAAATG AGAGAGAAGG GAGTGAAGCT GTTGCAAGCA
    CAGAAGCTGG TGCAATTCTT ACGGGAATGT GAAGATGTCA TGGACTGGAT CAATGACAAG
    GAAGCAATAG TGACCTCAGA AGAGCTTGGA CAGGACTTAG AGCATGTTGA GGTTTTGCAG
    AAGAAATTTG AAGAGTTCCA AACAGATCTG GCAGCTCATG AGGAGAGAGT GAATGAAGTG
    AACCAGTTCG CTGGCAAACT TATCCAGGAA CAGCACCCTG AGGAGGAACT TATAAAGTCC
    AAACAGGATG AAGTAAATGC AAGCTGGCAG CGTCTTAAGG GGCTTGCCCA ACAGAGGCAA
    GGAAAACTCT TTGGGGCAGC TGAAGTTCAG CGCTTCAACA GGGATGTGGA TGAAACTATC
    AGCTGGATTA AGGAGAAAGG GCAGTTGATG GCCTCAGATG ACTTTGGCAG AGACTTGGCC
    AGCGTGCAAG CTTTACTACG TAAGCATGAA GGCCTGGAAA GAGATCTTGC AGCTTTGGAG
    GATAAGGTTA AGGCCCTCTG TGCAGAAGCT GACCGTTTGC AGCAGTCTCA CCCTATAAAT
    GCTTCTCAAA TTCAAGTGAA ACGGGAGGAA CTGATTGCCA ACTGGGAGCA GATCCGAACT
    CTGGCAGCAG AGAGGCATGC TCGCCTTAAT GACTCCTACA GGTTGCAGCG CTTTCTTGCA
    GATTTCCGGG ACCTCACTAG CTGGGTAACT GAGATGAAGG CTCTGATAAA TGCTGATGAG
    CTTGCCAATG ATGTGGCTGG AGCAGAAGCC CTTCTAGATA GACATCAGGA ACATAAGGGA
    GAAATTGATG CTCATGAGGA TAGCTTCAGA TCTGCTGATG AGTCTGGGCA GGCTTTGCTT
    GCAGCTGGGC ACTATGCTTC TGATGAAGTT AAAGAAAAGC TGACTATCCT CTCAGATGAA
    AGATCAGCCT TGCTGGAACT ATGGGAGCTT CGCAGGCAAC AGTATGAGCA GTGCATGGAT
    CTGCAGCTTT TCTACAGAGA TACTGAACAA GTTGACAACT GGATGAGCAA ACAAGAAGCT
    TTTCTGCTGA ACGAAGACCT TGGTGATTCT CTGGATAGCG TGGAGGCTCT TCTAAAGAAG
    CATGAAGATT TTGAGAAATC CCTAAGTGCT CAAGAGGAGA AGATCACAGC ACTGGATGAG
    TTTGCTACTA AACTGATTCA AAATAACCAT TATGCCATGG ATGACGTTGC TACGCGCAGA
    GATGCTCTTC TGAGTCGCCG AAATGCTCTT CATGAAAGAG CCATGAAGCG CCGTGCCCAA
    CTGGCAGACT CTTTCCATCT CCAGCAGTTT TTCAGGGACT CTGATGAACT GAAGAGTTGG
    GTTAATGAAA AAATGAAAAC TGCTACCGAT GAGGCTTACA AGGATCCATC CAACTTGCAA
    GGTAAAGTTC AGAAGCATCA GGCTTTTGAA GCAGAGCTTT CTGCTAATCA GAGTCGTATT
    GATGCACTGG AGAAAGCTGG CCAGAAGTTG ATTGATGTCA ATCACTATGC ATCTGATGAG
    GTGGCAGCTC GCATGAATGA AGTCATCAGC TTGTGGAAGA AACTTCTGGA AGCCACCGAG
    CTCAAAGGTA TAAAGCTGCG TGAAGCCAAT CAACAGCAGC AATTTAATCG CAATGTAGAA
    GACATTGAGT TGTGGCTGTA TGAAGTGGAG GGTCACTTGG CTTCTGATGA TTATGGAAAA
    GATCTTACCA GCGTTCAGAA TCTTCAGAAG AAACATGCCC TGCTAGAGGC AGATGTTGCT
    GCCCATCAGG ATCGGATAGA TGGCATTACC ATCCAGGCAC GCCAGTTCCA AGATGCTGGG
    CACTTTGATG CTGACAATAT CAAGAAGAAA CAAGAAGCTT TAGTAGCTCG TTATGAAGCT
    CTGAAGGATC CTATGGTGGC TCGCAAGCAG AAACTTGCAG ATTCTCTTCG CCTGCAGCAG
    CTTTTCCGTG ACATCGAGGA TGAAGAGACC TGGATCAGGG AAAAGGAACC TATTGCAGCC
    TCAACAAACC GAGGCAAGGA CTTAATTGGT GTCCAGAATC TGCTAAAGAA GCACCAGGCT
    TTGCAGGCAG AAATTGCAGG CCATGAGCCT CGCATTAAAG CAGTCACACA AAAAGGAAAT
    GCGATGGTGG AAGAGGGACA TTTTGCTGCT GAAGATGTGA AGATCAAATT GAATGAACTA
    AACCAAAAAT GGGACTCTCT GAAAGCCAAG GCATCTCAAC GGCGACAGGA TCTAGAGGAT
    TCTCTGCAAG CTCAGCAGTA TTTTGCTGAT GCTAATGAGG CAGAATCATG GATGAGGGAA
    AAGGAGCCCA TTGTAGGCAG CACGGATTAT GGAAAGGATG AAGACTCTGC TGAGGCTCTC
    CTGAAGAAGC ATGAAGCTTT GATGTCTGAT CTTTCTGCTT ACGGCAGTAG CATACAGGCA
    TTAAGGGAAC AGGCCCAGTC CTGCAGGCAA CAAGTTGCTC CCACTGATGA TGAAACTGGA
    AAAGAGCTTG TGCTAGCACT CTATGATTAC CAAGAGAAGA GTCCTCGGGA GGTGACTATG
    AAGAAAGGAG ATATTCTAAC CCTGCTCAAC AGCACCAACA AGGACTGGTG GAAGGTTGAA
    GTTAACGATC GTCAGGGCTT TGTACCAGCT GCCTATGTGA AAAAACTAGA TCCTGCCCAG
    TCTGCATCCC GAGAGAATCT TCTAGAAGAG CAAGGCAGCA TAGCATTGCG ACAGGAGCAA
    ATTGACAACC AGACTCTCAT TACTAAGGAA GTCGGCAGTG TATCTCTGCG TATGAAACAG
    GTCGAAGAAC TGTATCATTC CCTTCTTGAG CTGGGAGAAA AACGTAAAGG CATGCTAGAA
    AAAAGCTGCA AGAAGTTTAT GCTTTTCCGT GAGGCTAATG AGCTTCAACA GTGGATCAAT
    GAGAAGGAAG CTGCGCTCAC TAATGAGGAA GTGGGTGCTG ATTTGGAGCA GGTGGAGGTG
    CTACAGAAGA AGTTTGATGA TTTTCAGAAG GATTTAAAAG CTAATGAGTC ACGACTGAAG
    GACATAAACA AGGTTGCAAA TGACCTGGAG TCTGAAGGGC TGATGGCAGA GGAAGTGCAA
    GCAGTAGAGC ACCAGGAAGT CTATGGAATG ATGCCCAGAG ATGAAACTGA TTCTAAGACA
    GCCTCTCCTT GGAAGTCTGC ACGTATGATG GTACACACAG TGGCAACGTT TAACTCAATC
    AAGGAACTGA ATGAACGCTG GAGATCCCTG CAGCAGTTGG CAGAGGAGCG AAGCCAGCTG
    TTGGGCAGCG CTCATGAAGT CCAGAGGTTC CACAGAGATG CTGATGAAAC AAAAGAATGG
    ATAGAAGAGA AGAATCAAGC ATTAAATACC GACAACTATG GACATGACTT GGCCAGTGTG
    CAGGCTCTGC AGCGCAAGCA TGAAGGCTTT GAGAGAGACT TGGCAGCTCT TGGAGACAAG
    GTGAATTCTC TTGGTGAAAC TGCCCAGCGT CTGATCCAGT CACATCCAGA ATCAGCTGAA
    GATCTTCAAG AAAAATGTAC TGAGTTGAAC CAAGCCTGGA GTAGTCTGGG AAAACGTGCT
    GACCAACGCA AAGAGAAGCT TGGAGATTCT CATGACCTGC AGCGTTTCCT CAGTGATTTT
    AGAGACCTTA TGTCTTGGAT CAATGGAATC CGGGGTCTGG TCTCCTCAGA TGAACTTGCA
    AAAGATGTGA CTGGAGCTGA AGCTCTGTTG GAAAGGCATC AGGAGCACCG TACTGAAATA
    GATGCACGGG CTGGCACTTT TCAGGCATTT GAACAGTTTG GACAGCAGCT TCTGGCTCGT
    GGACACTATG CCAGCCCAGA GATTAAGGAG AAACTGGATA TTCTAGATCA AGAACGGACA
    GACCTAGAGA AGGCCTGGGT CCAGCGCAGA ATGATGCTAG ACCAGTGCTT AGAACTACAG
    CTGTTTCATC GGGATTGTGA ACAAGCTGAA AACTGGATGG CTGCCCGAGA GGCGTTCCTA
    AATACAGAAG ACAAAGGAGA CTCCTTAGAC AGCGTGGAGG CACTCATCAA GAAGCATGAA
    GATTTCGATA AAGCAATCAA TGTCCAGGAA GAGAAAATTG CTGTCTTGCA GTCTTTTGCT
    GACCAACTGA TTGCTGCTGA TCATTATGCA AAAGGAGTCA TTGCTAACAG ACGCAATGAG
    GTTCTGGACA GGTGGCGTCG TCTGAAGGCT CAGATGATTG AGAAGAGATC TAAGCTGGGA
    GAATCTCAGA CCCTCCAACA GTTCAGTCGT GATGTTGATG AAATAGAAGC TTGGATCAGT
    GAAAAGCTTC AAACTGCAAG TGATGAGTCA TATAAGGATC CCACAAACAT CCAGCTTTCC
    AAACTGCTGA GCAAGCACCA GAAGCACCAA GCCTTTGAAG CTGAGCTCCA CGCCAACGCA
    GATCGGATTC GTGGAGTCAT TGAAATGGGG AACTCTCTTA TTGAAAGAGG AGCGTGTGCT
    GGCAGCGAGG ATGCTGTGAA GGCTCGGCTG GCTGCCCTGG CTGACCAATG GGAGTTCCTG
    GTCCAGAAGT CATCAGAGAA GAGTCAGAAA CTGAAAGAAG CAAATAAACA GCAGAATTTC
    AATACCGGCA TCAAGGACTT TGATTTCTGG CTTTCAGAGG TGGAAGCTTT GTTGGCATCT
    GAAGACTATG GGAAGGACTT GGCATCAGTG AACAACCTTC TGAAGAAGCA CCAATTACTG
    GAAGCTGATA TATCTGCTCA TGAGGATCGG CTGAAGGACC TGAACAGCCA GGCTGACAGT
    TTGATGACCA GCAGTGCTTT CGATACCTCC CAAGTAAAGG ATAAACGTGA AACTATAAAT
    GGGCGCTTCC AGAGAATCAA GAGCATGGCA GCTGCCCGCC GTGCGAAGCT CAACGAGTCG
    CACCGCTTGC ATCAGTTCTT CCGTGACATG GATGATGAGG AGTCCTGGAT CAAAGAGAAG
    AAACTGTTGG TGAGCTCAGA GGACTATGGC AGAGACCTGA CTGGTGTGCA GAACCTGAGG
    AAGAAACATA AGCGCTTGGA AGCAGAATTA GCTGCCCATG AACCTGCTAT CCAGGGTGTT
    CTAGACACTG GCAAGAAGCT TTCAGATGAT AACACAATTG GGAAGGAGGA GATACAGCAA
    AGACTGGCTC AGTTTGTGGA CCACTGGAAA GAGTTAAAAC AGCTGGCAGC TGCTCGGGGG
    CAGCGTCTGG AGGAGTCCCT GGAGTACCAA CAGTTTGTAG CAAATGTTGA GGAAGAGGAG
    GCATGGATCA ACGAGAAAAT GACATTGGTA GCCAGTGAGG ATTATGGAGA CACACTTGCT
    GCTATCCAGG GCTTGCTGAA GAAGCATGAA GCATTCGAGA CTGACTTTAC TGTCCACAAA
    GACAGAGTGA ATGATGTTTG CGCTAATGGA GAGGATCTCA TTAAAAAGAA TAATCACCAT
    GTGGAGAACA TTACTGCTAA GATGAAGGGC CTCAAGGGCA AGGTATCAGA TCTGGAGAAA
    GCCGCAGCTC AGAGGAAAGC CAAACTGGAT GAGAACTCTG CCTTCCTCCA GTTCAACTGG
    AAAGCAGATG TGGTGGAGTC ATGGATAGGT GAGAAGGAAA ACAGTCTGAA GACAGATGAT
    TATGGCCGTG ACCTCTCCTC TGTGCAAACA CTACTCACCA AACAGGAAAC CTTTGATGCT
    GGACTTCAGG CTTTCCAGCA GGAGGGAATT GCAAACATCA CTGCTCTGAA AGACCAGCTA
    CTGGCAGCCA AACATATCCA ATCAAAGGCC ATTGAGGTTC GTCACGCTTC CTTGATGAAA
    CGCTGGAATC AGCTGCTTGC TAATTCTGCA GCCAGGAAAA AGAAACTCTT GGAGGCTCAG
    GAGCACTTCA GAAAGGTTGA AGATCTCTTC TTGACTTTTG CCAAGAAGGC CTCTGCCTTC
    AACAGTTGGT TTGAGAATGC TGAGGAGGAC CTGACGGATC CCGTGCGCTG CAATTCCCTG
    GAAGAAATCA AAGCACTGCG AGAAGCCCAC GATGCGTTCC GTTCCTCACT TAGCTCTGCC
    CAAGCTGACT TCAACCAGCT GGCAGAGCTT GATCGGCAGA TCAAGAGCTT CCGTGTAGCC
    TCCAACCCGT ACACTTGGTT TACTATGGAG GCTCTTGAAG AAACTTGGAG GAATCTGCAG
    AAAATTATCA AGGAGCGTGA ATTGGAGTTG CAGAAGGAGC AGCGGAGGCA GGAAGAAAAT
    GACAAGTTGC GCCAGGAGTT TGCTCAGCAT GCTAATGCCT TCCACCAGTG GATTCAGGAG
    ACAAGGACTT ACCTGCTAGA TGGGTCCTGC ATGGTGGAGG AGTCGGGAAC ACTGGAATCC
    CAGCTGGAAG CTACTAAACG CAAGCACCAG GAGATCCGGG CTATGAGGAG CCAGCTGAAG
    AAGATTGAGG ACCTTGGAGC AGCCATGGAA GAGGCACTTA TCTTGGACAA CAAATACACA
    GAGCACAGCA CCGTGGGGCT GGCGCAGCAG TGGGACCAGC TTGACCAGCT GGGAATGAGA
    ATGCAGCACA ATCTGGAACA GCAGATTCAA GCTCGAAACA CAACTGGAGT CACAGAGGAG
    GCCCTGAAGG AGTTCAGCAT GATGTTCAAG CACTTTGACA AGGACAAATC TGGACGTCTT
    AATCACCAGG AGTTTAAGTC TTGCTTGCGC TCCCTTGGCT ATGACCTGCC CATGGTTGAG
    GAAGGAGAGC CTGACCCCGA ATTTGAGTCT ATTCTTGACA CTGTTGATCC CAACAGGGAT
    GGCCACGTCT CGCTGCAGGA GTACATGGCG TTCATGATCA GCAGGGAAAC AGAGAACGTG
    AAATCCAGCG AGGAGATTGA GAGTGCTTTC CGTGCCCTCA GCTCGGAGGG GAAGCCTTAC
    GTGACCAAGG AGGAGCTCTA CCAGAACCTG ACCCGGGAGC AGGCCGACTA CTGCATTTCT
    CACATGAAGC CCTACATGGA TGGCAAGGGC AGAGAACTTC CTTCTGCCTA CGACTACATA
    GAATTTACAC GTTCACTCTT TGTGAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa43318
    Clone ID Related Accession (Same CDS sequence) XM_015279292.2 , XM_015279292.1
    Accession Version XM_015279292.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7455bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015279292.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
    TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG
    TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC
    TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT
    GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT
    GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG
    ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAA CTCGTCTACA GGAGCTGCAC
    CGACTATGGG AGTTACTGCT GGAAAAAATG AGAGAGAAGG GAGTGAAGCT GTTGCAAGCA
    CAGAAGCTGG TGCAATTCTT ACGGGAATGT GAAGATGTCA TGGACTGGAT CAATGACAAG
    GAAGCAATAG TGACCTCAGA AGAGCTTGGA CAGGACTTAG AGCATGTTGA GGTTTTGCAG
    AAGAAATTTG AAGAGTTCCA AACAGATCTG GCAGCTCATG AGGAGAGAGT GAATGAAGTG
    AACCAGTTCG CTGGCAAACT TATCCAGGAA CAGCACCCTG AGGAGGAACT TATAAAGTCC
    AAACAGGATG AAGTAAATGC AAGCTGGCAG CGTCTTAAGG GGCTTGCCCA ACAGAGGCAA
    GGAAAACTCT TTGGGGCAGC TGAAGTTCAG CGCTTCAACA GGGATGTGGA TGAAACTATC
    AGCTGGATTA AGGAGAAAGG GCAGTTGATG GCCTCAGATG ACTTTGGCAG AGACTTGGCC
    AGCGTGCAAG CTTTACTACG TAAGCATGAA GGCCTGGAAA GAGATCTTGC AGCTTTGGAG
    GATAAGGTTA AGGCCCTCTG TGCAGAAGCT GACCGTTTGC AGCAGTCTCA CCCTATAAAT
    GCTTCTCAAA TTCAAGTGAA ACGGGAGGAA CTGATTGCCA ACTGGGAGCA GATCCGAACT
    CTGGCAGCAG AGAGGCATGC TCGCCTTAAT GACTCCTACA GGTTGCAGCG CTTTCTTGCA
    GATTTCCGGG ACCTCACTAG CTGGGTAACT GAGATGAAGG CTCTGATAAA TGCTGATGAG
    CTTGCCAATG ATGTGGCTGG AGCAGAAGCC CTTCTAGATA GACATCAGGA ACATAAGGGA
    GAAATTGATG CTCATGAGGA TAGCTTCAGA TCTGCTGATG AGTCTGGGCA GGCTTTGCTT
    GCAGCTGGGC ACTATGCTTC TGATGAAGTT AAAGAAAAGC TGACTATCCT CTCAGATGAA
    AGATCAGCCT TGCTGGAACT ATGGGAGCTT CGCAGGCAAC AGTATGAGCA GTGCATGGAT
    CTGCAGCTTT TCTACAGAGA TACTGAACAA GTTGACAACT GGATGAGCAA ACAAGAAGCT
    TTTCTGCTGA ACGAAGACCT TGGTGATTCT CTGGATAGCG TGGAGGCTCT TCTAAAGAAG
    CATGAAGATT TTGAGAAATC CCTAAGTGCT CAAGAGGAGA AGATCACAGC ACTGGATGAG
    TTTGCTACTA AACTGATTCA AAATAACCAT TATGCCATGG ATGACGTTGC TACGCGCAGA
    GATGCTCTTC TGAGTCGCCG AAATGCTCTT CATGAAAGAG CCATGAAGCG CCGTGCCCAA
    CTGGCAGACT CTTTCCATCT CCAGCAGTTT TTCAGGGACT CTGATGAACT GAAGAGTTGG
    GTTAATGAAA AAATGAAAAC TGCTACCGAT GAGGCTTACA AGGATCCATC CAACTTGCAA
    GGTAAAGTTC AGAAGCATCA GGCTTTTGAA GCAGAGCTTT CTGCTAATCA GAGTCGTATT
    GATGCACTGG AGAAAGCTGG CCAGAAGTTG ATTGATGTCA ATCACTATGC ATCTGATGAG
    GTGGCAGCTC GCATGAATGA AGTCATCAGC TTGTGGAAGA AACTTCTGGA AGCCACCGAG
    CTCAAAGGTA TAAAGCTGCG TGAAGCCAAT CAACAGCAGC AATTTAATCG CAATGTAGAA
    GACATTGAGT TGTGGCTGTA TGAAGTGGAG GGTCACTTGG CTTCTGATGA TTATGGAAAA
    GATCTTACCA GCGTTCAGAA TCTTCAGAAG AAACATGCCC TGCTAGAGGC AGATGTTGCT
    GCCCATCAGG ATCGGATAGA TGGCATTACC ATCCAGGCAC GCCAGTTCCA AGATGCTGGG
    CACTTTGATG CTGACAATAT CAAGAAGAAA CAAGAAGCTT TAGTAGCTCG TTATGAAGCT
    CTGAAGGATC CTATGGTGGC TCGCAAGCAG AAACTTGCAG ATTCTCTTCG CCTGCAGCAG
    CTTTTCCGTG ACATCGAGGA TGAAGAGACC TGGATCAGGG AAAAGGAACC TATTGCAGCC
    TCAACAAACC GAGGCAAGGA CTTAATTGGT GTCCAGAATC TGCTAAAGAA GCACCAGGCT
    TTGCAGGCAG AAATTGCAGG CCATGAGCCT CGCATTAAAG CAGTCACACA AAAAGGAAAT
    GCGATGGTGG AAGAGGGACA TTTTGCTGCT GAAGATGTGA AGATCAAATT GAATGAACTA
    AACCAAAAAT GGGACTCTCT GAAAGCCAAG GCATCTCAAC GGCGACAGGA TCTAGAGGAT
    TCTCTGCAAG CTCAGCAGTA TTTTGCTGAT GCTAATGAGG CAGAATCATG GATGAGGGAA
    AAGGAGCCCA TTGTAGGCAG CACGGATTAT GGAAAGGATG AAGACTCTGC TGAGGCTCTC
    CTGAAGAAGC ATGAAGCTTT GATGTCTGAT CTTTCTGCTT ACGGCAGTAG CATACAGGCA
    TTAAGGGAAC AGGCCCAGTC CTGCAGGCAA CAAGTTGCTC CCACTGATGA TGAAACTGGA
    AAAGAGCTTG TGCTAGCACT CTATGATTAC CAAGAGAAGA GTCCTCGGGA GGTGACTATG
    AAGAAAGGAG ATATTCTAAC CCTGCTCAAC AGCACCAACA AGGACTGGTG GAAGGTTGAA
    GTTAACGATC GTCAGGGCTT TGTACCAGCT GCCTATGTGA AAAAACTAGA TCCTGCCCAG
    TCTGCATCCC GAGAGAATCT TCTAGAAGAG CAAGGCAGCA TAGCATTGCG ACAGGAGCAA
    ATTGACAACC AGACTCTCAT TACTAAGGAA GTCGGCAGTG TATCTCTGCG TATGAAACAG
    GTCGAAGAAC TGTATCATTC CCTTCTTGAG CTGGGAGAAA AACGTAAAGG CATGCTAGAA
    AAAAGCTGCA AGAAGTTTAT GCTTTTCCGT GAGGCTAATG AGCTTCAACA GTGGATCAAT
    GAGAAGGAAG CTGCGCTCAC TAATGAGGAA GTGGGTGCTG ATTTGGAGCA GGTGGAGGTG
    CTACAGAAGA AGTTTGATGA TTTTCAGAAG GATTTAAAAG CTAATGAGTC ACGACTGAAG
    GACATAAACA AGGTTGCAAA TGACCTGGAG TCTGAAGGGC TGATGGCAGA GGAAGTGCAA
    GCAGTAGAGC ACCAGGAAGT CTATGGAATG ATGCCCAGAG ATGAAACTGA TTCTAAGACA
    GCCTCTCCTT GGAAGTCTGC ACGTATGATG GTACACACAG TGGCAACGTT TAACTCAATC
    AAGGAACTGA ATGAACGCTG GAGATCCCTG CAGCAGTTGG CAGAGGAGCG AAGCCAGCTG
    TTGGGCAGCG CTCATGAAGT CCAGAGGTTC CACAGAGATG CTGATGAAAC AAAAGAATGG
    ATAGAAGAGA AGAATCAAGC ATTAAATACC GACAACTATG GACATGACTT GGCCAGTGTG
    CAGGCTCTGC AGCGCAAGCA TGAAGGCTTT GAGAGAGACT TGGCAGCTCT TGGAGACAAG
    GTGAATTCTC TTGGTGAAAC TGCCCAGCGT CTGATCCAGT CACATCCAGA ATCAGCTGAA
    GATCTTCAAG AAAAATGTAC TGAGTTGAAC CAAGCCTGGA GTAGTCTGGG AAAACGTGCT
    GACCAACGCA AAGAGAAGCT TGGAGATTCT CATGACCTGC AGCGTTTCCT CAGTGATTTT
    AGAGACCTTA TGTCTTGGAT CAATGGAATC CGGGGTCTGG TCTCCTCAGA TGAACTTGCA
    AAAGATGTGA CTGGAGCTGA AGCTCTGTTG GAAAGGCATC AGGAGCACCG TACTGAAATA
    GATGCACGGG CTGGCACTTT TCAGGCATTT GAACAGTTTG GACAGCAGCT TCTGGCTCGT
    GGACACTATG CCAGCCCAGA GATTAAGGAG AAACTGGATA TTCTAGATCA AGAACGGACA
    GACCTAGAGA AGGCCTGGGT CCAGCGCAGA ATGATGCTAG ACCAGTGCTT AGAACTACAG
    CTGTTTCATC GGGATTGTGA ACAAGCTGAA AACTGGATGG CTGCCCGAGA GGCGTTCCTA
    AATACAGAAG ACAAAGGAGA CTCCTTAGAC AGCGTGGAGG CACTCATCAA GAAGCATGAA
    GATTTCGATA AAGCAATCAA TGTCCAGGAA GAGAAAATTG CTGTCTTGCA GTCTTTTGCT
    GACCAACTGA TTGCTGCTGA TCATTATGCA AAAGGAGTCA TTGCTAACAG ACGCAATGAG
    GTTCTGGACA GGTGGCGTCG TCTGAAGGCT CAGATGATTG AGAAGAGATC TAAGCTGGGA
    GAATCTCAGA CCCTCCAACA GTTCAGTCGT GATGTTGATG AAATAGAAGC TTGGATCAGT
    GAAAAGCTTC AAACTGCAAG TGATGAGTCA TATAAGGATC CCACAAACAT CCAGAGCAAG
    CACCAGAAGC ACCAAGCCTT TGAAGCTGAG CTCCACGCCA ACGCAGATCG GATTCGTGGA
    GTCATTGAAA TGGGGAACTC TCTTATTGAA AGAGGAGCGT GTGCTGGCAG CGAGGATGCT
    GTGAAGGCTC GGCTGGCTGC CCTGGCTGAC CAATGGGAGT TCCTGGTCCA GAAGTCATCA
    GAGAAGAGTC AGAAACTGAA AGAAGCAAAT AAACAGCAGA ATTTCAATAC CGGCATCAAG
    GACTTTGATT TCTGGCTTTC AGAGGTGGAA GCTTTGTTGG CATCTGAAGA CTATGGGAAG
    GACTTGGCAT CAGTGAACAA CCTTCTGAAG AAGCACCAAT TACTGGAAGC TGATATATCT
    GCTCATGAGG ATCGGCTGAA GGACCTGAAC AGCCAGGCTG ACAGTTTGAT GACCAGCAGT
    GCTTTCGATA CCTCCCAAGT AAAGGATAAA CGTGAAACTA TAAATGGGCG CTTCCAGAGA
    ATCAAGAGCA TGGCAGCTGC CCGCCGTGCG AAGCTCAACG AGTCGCACCG CTTGCATCAG
    TTCTTCCGTG ACATGGATGA TGAGGAGTCC TGGATCAAAG AGAAGAAACT GTTGGTGAGC
    TCAGAGGACT ATGGCAGAGA CCTGACTGGT GTGCAGAACC TGAGGAAGAA ACATAAGCGC
    TTGGAAGCAG AATTAGCTGC CCATGAACCT GCTATCCAGG GTGTTCTAGA CACTGGCAAG
    AAGCTTTCAG ATGATAACAC AATTGGGAAG GAGGAGATAC AGCAAAGACT GGCTCAGTTT
    GTGGACCACT GGAAAGAGTT AAAACAGCTG GCAGCTGCTC GGGGGCAGCG TCTGGAGGAG
    TCCCTGGAGT ACCAACAGTT TGTAGCAAAT GTTGAGGAAG AGGAGGCATG GATCAACGAG
    AAAATGACAT TGGTAGCCAG TGAGGATTAT GGAGACACAC TTGCTGCTAT CCAGGGCTTG
    CTGAAGAAGC ATGAAGCATT CGAGACTGAC TTTACTGTCC ACAAAGACAG AGTGAATGAT
    GTTTGCGCTA ATGGAGAGGA TCTCATTAAA AAGAATAATC ACCATGTGGA GAACATTACT
    GCTAAGATGA AGGGCCTCAA GGGCAAGGTA TCAGATCTGG AGAAAGCCGC AGCTCAGAGG
    AAAGCCAAAC TGGATGAGAA CTCTGCCTTC CTCCAGTTCA ACTGGAAAGC AGATGTGGTG
    GAGTCATGGA TAGGTGAGAA GGAAAACAGT CTGAAGACAG ATGATTATGG CCGTGACCTC
    TCCTCTGTGC AAACACTACT CACCAAACAG GAAACCTTTG ATGCTGGACT TCAGGCTTTC
    CAGCAGGAGG GAATTGCAAA CATCACTGCT CTGAAAGACC AGCTACTGGC AGCCAAACAT
    ATCCAATCAA AGGCCATTGA GGTTCGTCAC GCTTCCTTGA TGAAACGCTG GAATCAGCTG
    CTTGCTAATT CTGCAGCCAG GAAAAAGAAA CTCTTGGAGG CTCAGGAGCA CTTCAGAAAG
    GTTGAAGATC TCTTCTTGAC TTTTGCCAAG AAGGCCTCTG CCTTCAACAG TTGGTTTGAG
    AATGCTGAGG AGGACCTGAC GGATCCCGTG CGCTGCAATT CCCTGGAAGA AATCAAAGCA
    CTGCGAGAAG CCCACGATGC GTTCCGTTCC TCACTTAGCT CTGCCCAAGC TGACTTCAAC
    CAGCTGGCAG AGCTTGATCG GCAGATCAAG AGCTTCCGTG TAGCCTCCAA CCCGTACACT
    TGGTTTACTA TGGAGGCTCT TGAAGAAACT TGGAGGAATC TGCAGAAAAT TATCAAGGAG
    CGTGAATTGG AGTTGCAGAA GGAGCAGCGG AGGCAGGAAG AAAATGACAA GTTGCGCCAG
    GAGTTTGCTC AGCATGCTAA TGCCTTCCAC CAGTGGATTC AGGAGACAAG GACTTACCTG
    CTAGATGGGT CCTGCATGGT GGAGGAGTCG GGAACACTGG AATCCCAGCT GGAAGCTACT
    AAACGCAAGC ACCAGGAGAT CCGGGCTATG AGGAGCCAGC TGAAGAAGAT TGAGGACCTT
    GGAGCAGCCA TGGAAGAGGC ACTTATCTTG GACAACAAAT ACACAGAGCA CAGCACCGTG
    GGGCTGGCGC AGCAGTGGGA CCAGCTTGAC CAGCTGGGAA TGAGAATGCA GCACAATCTG
    GAACAGCAGA TTCAAGCTCG AAACACAACT GGAGTCACAG AGGAGGCCCT GAAGGAGTTC
    AGCATGATGT TCAAGCACTT TGACAAGGAC AAATCTGGAC GTCTTAATCA CCAGGAGTTT
    AAGTCTTGCT TGCGCTCCCT TGGCTATGAC CTGCCCATGG TTGAGGAAGG AGAGCCTGAC
    CCCGAATTTG AGTCTATTCT TGACACTGTT GATCCCAACA GGGATGGCCA CGTCTCGCTG
    CAGGAGTACA TGGCGTTCAT GATCAGCAGG GAAACAGAGA ACGTGAAATC CAGCGAGGAG
    ATTGAGAGTG CTTTCCGTGC CCTCAGCTCG GAGGGGAAGC CTTACGTGAC CAAGGAGGAG
    CTCTACCAGA ACCTGACCCG GGAGCAGGCC GACTACTGCA TTTCTCACAT GAAGCCCTAC
    ATGGATGGCA AGGGCAGAGA ACTTCCTTCT GCCTACGACT ACATAGAATT TACACGTTCA
    CTCTTTGTGA ATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015134778.1
    CDS8..7462
    Translation

    Target ORF information:

    RefSeq Version XM_015279292.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015279292.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
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    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
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    6781
    6841
    6901
    6961
    7021
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    7141
    7201
    7261
    7321
    7381
    7441
    ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
    TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG
    TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC
    TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT
    GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT
    GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG
    ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAA CTCGTCTACA GGAGCTGCAC
    CGACTATGGG AGTTACTGCT GGAAAAAATG AGAGAGAAGG GAGTGAAGCT GTTGCAAGCA
    CAGAAGCTGG TGCAATTCTT ACGGGAATGT GAAGATGTCA TGGACTGGAT CAATGACAAG
    GAAGCAATAG TGACCTCAGA AGAGCTTGGA CAGGACTTAG AGCATGTTGA GGTTTTGCAG
    AAGAAATTTG AAGAGTTCCA AACAGATCTG GCAGCTCATG AGGAGAGAGT GAATGAAGTG
    AACCAGTTCG CTGGCAAACT TATCCAGGAA CAGCACCCTG AGGAGGAACT TATAAAGTCC
    AAACAGGATG AAGTAAATGC AAGCTGGCAG CGTCTTAAGG GGCTTGCCCA ACAGAGGCAA
    GGAAAACTCT TTGGGGCAGC TGAAGTTCAG CGCTTCAACA GGGATGTGGA TGAAACTATC
    AGCTGGATTA AGGAGAAAGG GCAGTTGATG GCCTCAGATG ACTTTGGCAG AGACTTGGCC
    AGCGTGCAAG CTTTACTACG TAAGCATGAA GGCCTGGAAA GAGATCTTGC AGCTTTGGAG
    GATAAGGTTA AGGCCCTCTG TGCAGAAGCT GACCGTTTGC AGCAGTCTCA CCCTATAAAT
    GCTTCTCAAA TTCAAGTGAA ACGGGAGGAA CTGATTGCCA ACTGGGAGCA GATCCGAACT
    CTGGCAGCAG AGAGGCATGC TCGCCTTAAT GACTCCTACA GGTTGCAGCG CTTTCTTGCA
    GATTTCCGGG ACCTCACTAG CTGGGTAACT GAGATGAAGG CTCTGATAAA TGCTGATGAG
    CTTGCCAATG ATGTGGCTGG AGCAGAAGCC CTTCTAGATA GACATCAGGA ACATAAGGGA
    GAAATTGATG CTCATGAGGA TAGCTTCAGA TCTGCTGATG AGTCTGGGCA GGCTTTGCTT
    GCAGCTGGGC ACTATGCTTC TGATGAAGTT AAAGAAAAGC TGACTATCCT CTCAGATGAA
    AGATCAGCCT TGCTGGAACT ATGGGAGCTT CGCAGGCAAC AGTATGAGCA GTGCATGGAT
    CTGCAGCTTT TCTACAGAGA TACTGAACAA GTTGACAACT GGATGAGCAA ACAAGAAGCT
    TTTCTGCTGA ACGAAGACCT TGGTGATTCT CTGGATAGCG TGGAGGCTCT TCTAAAGAAG
    CATGAAGATT TTGAGAAATC CCTAAGTGCT CAAGAGGAGA AGATCACAGC ACTGGATGAG
    TTTGCTACTA AACTGATTCA AAATAACCAT TATGCCATGG ATGACGTTGC TACGCGCAGA
    GATGCTCTTC TGAGTCGCCG AAATGCTCTT CATGAAAGAG CCATGAAGCG CCGTGCCCAA
    CTGGCAGACT CTTTCCATCT CCAGCAGTTT TTCAGGGACT CTGATGAACT GAAGAGTTGG
    GTTAATGAAA AAATGAAAAC TGCTACCGAT GAGGCTTACA AGGATCCATC CAACTTGCAA
    GGTAAAGTTC AGAAGCATCA GGCTTTTGAA GCAGAGCTTT CTGCTAATCA GAGTCGTATT
    GATGCACTGG AGAAAGCTGG CCAGAAGTTG ATTGATGTCA ATCACTATGC ATCTGATGAG
    GTGGCAGCTC GCATGAATGA AGTCATCAGC TTGTGGAAGA AACTTCTGGA AGCCACCGAG
    CTCAAAGGTA TAAAGCTGCG TGAAGCCAAT CAACAGCAGC AATTTAATCG CAATGTAGAA
    GACATTGAGT TGTGGCTGTA TGAAGTGGAG GGTCACTTGG CTTCTGATGA TTATGGAAAA
    GATCTTACCA GCGTTCAGAA TCTTCAGAAG AAACATGCCC TGCTAGAGGC AGATGTTGCT
    GCCCATCAGG ATCGGATAGA TGGCATTACC ATCCAGGCAC GCCAGTTCCA AGATGCTGGG
    CACTTTGATG CTGACAATAT CAAGAAGAAA CAAGAAGCTT TAGTAGCTCG TTATGAAGCT
    CTGAAGGATC CTATGGTGGC TCGCAAGCAG AAACTTGCAG ATTCTCTTCG CCTGCAGCAG
    CTTTTCCGTG ACATCGAGGA TGAAGAGACC TGGATCAGGG AAAAGGAACC TATTGCAGCC
    TCAACAAACC GAGGCAAGGA CTTAATTGGT GTCCAGAATC TGCTAAAGAA GCACCAGGCT
    TTGCAGGCAG AAATTGCAGG CCATGAGCCT CGCATTAAAG CAGTCACACA AAAAGGAAAT
    GCGATGGTGG AAGAGGGACA TTTTGCTGCT GAAGATGTGA AGATCAAATT GAATGAACTA
    AACCAAAAAT GGGACTCTCT GAAAGCCAAG GCATCTCAAC GGCGACAGGA TCTAGAGGAT
    TCTCTGCAAG CTCAGCAGTA TTTTGCTGAT GCTAATGAGG CAGAATCATG GATGAGGGAA
    AAGGAGCCCA TTGTAGGCAG CACGGATTAT GGAAAGGATG AAGACTCTGC TGAGGCTCTC
    CTGAAGAAGC ATGAAGCTTT GATGTCTGAT CTTTCTGCTT ACGGCAGTAG CATACAGGCA
    TTAAGGGAAC AGGCCCAGTC CTGCAGGCAA CAAGTTGCTC CCACTGATGA TGAAACTGGA
    AAAGAGCTTG TGCTAGCACT CTATGATTAC CAAGAGAAGA GTCCTCGGGA GGTGACTATG
    AAGAAAGGAG ATATTCTAAC CCTGCTCAAC AGCACCAACA AGGACTGGTG GAAGGTTGAA
    GTTAACGATC GTCAGGGCTT TGTACCAGCT GCCTATGTGA AAAAACTAGA TCCTGCCCAG
    TCTGCATCCC GAGAGAATCT TCTAGAAGAG CAAGGCAGCA TAGCATTGCG ACAGGAGCAA
    ATTGACAACC AGACTCTCAT TACTAAGGAA GTCGGCAGTG TATCTCTGCG TATGAAACAG
    GTCGAAGAAC TGTATCATTC CCTTCTTGAG CTGGGAGAAA AACGTAAAGG CATGCTAGAA
    AAAAGCTGCA AGAAGTTTAT GCTTTTCCGT GAGGCTAATG AGCTTCAACA GTGGATCAAT
    GAGAAGGAAG CTGCGCTCAC TAATGAGGAA GTGGGTGCTG ATTTGGAGCA GGTGGAGGTG
    CTACAGAAGA AGTTTGATGA TTTTCAGAAG GATTTAAAAG CTAATGAGTC ACGACTGAAG
    GACATAAACA AGGTTGCAAA TGACCTGGAG TCTGAAGGGC TGATGGCAGA GGAAGTGCAA
    GCAGTAGAGC ACCAGGAAGT CTATGGAATG ATGCCCAGAG ATGAAACTGA TTCTAAGACA
    GCCTCTCCTT GGAAGTCTGC ACGTATGATG GTACACACAG TGGCAACGTT TAACTCAATC
    AAGGAACTGA ATGAACGCTG GAGATCCCTG CAGCAGTTGG CAGAGGAGCG AAGCCAGCTG
    TTGGGCAGCG CTCATGAAGT CCAGAGGTTC CACAGAGATG CTGATGAAAC AAAAGAATGG
    ATAGAAGAGA AGAATCAAGC ATTAAATACC GACAACTATG GACATGACTT GGCCAGTGTG
    CAGGCTCTGC AGCGCAAGCA TGAAGGCTTT GAGAGAGACT TGGCAGCTCT TGGAGACAAG
    GTGAATTCTC TTGGTGAAAC TGCCCAGCGT CTGATCCAGT CACATCCAGA ATCAGCTGAA
    GATCTTCAAG AAAAATGTAC TGAGTTGAAC CAAGCCTGGA GTAGTCTGGG AAAACGTGCT
    GACCAACGCA AAGAGAAGCT TGGAGATTCT CATGACCTGC AGCGTTTCCT CAGTGATTTT
    AGAGACCTTA TGTCTTGGAT CAATGGAATC CGGGGTCTGG TCTCCTCAGA TGAACTTGCA
    AAAGATGTGA CTGGAGCTGA AGCTCTGTTG GAAAGGCATC AGGAGCACCG TACTGAAATA
    GATGCACGGG CTGGCACTTT TCAGGCATTT GAACAGTTTG GACAGCAGCT TCTGGCTCGT
    GGACACTATG CCAGCCCAGA GATTAAGGAG AAACTGGATA TTCTAGATCA AGAACGGACA
    GACCTAGAGA AGGCCTGGGT CCAGCGCAGA ATGATGCTAG ACCAGTGCTT AGAACTACAG
    CTGTTTCATC GGGATTGTGA ACAAGCTGAA AACTGGATGG CTGCCCGAGA GGCGTTCCTA
    AATACAGAAG ACAAAGGAGA CTCCTTAGAC AGCGTGGAGG CACTCATCAA GAAGCATGAA
    GATTTCGATA AAGCAATCAA TGTCCAGGAA GAGAAAATTG CTGTCTTGCA GTCTTTTGCT
    GACCAACTGA TTGCTGCTGA TCATTATGCA AAAGGAGTCA TTGCTAACAG ACGCAATGAG
    GTTCTGGACA GGTGGCGTCG TCTGAAGGCT CAGATGATTG AGAAGAGATC TAAGCTGGGA
    GAATCTCAGA CCCTCCAACA GTTCAGTCGT GATGTTGATG AAATAGAAGC TTGGATCAGT
    GAAAAGCTTC AAACTGCAAG TGATGAGTCA TATAAGGATC CCACAAACAT CCAGAGCAAG
    CACCAGAAGC ACCAAGCCTT TGAAGCTGAG CTCCACGCCA ACGCAGATCG GATTCGTGGA
    GTCATTGAAA TGGGGAACTC TCTTATTGAA AGAGGAGCGT GTGCTGGCAG CGAGGATGCT
    GTGAAGGCTC GGCTGGCTGC CCTGGCTGAC CAATGGGAGT TCCTGGTCCA GAAGTCATCA
    GAGAAGAGTC AGAAACTGAA AGAAGCAAAT AAACAGCAGA ATTTCAATAC CGGCATCAAG
    GACTTTGATT TCTGGCTTTC AGAGGTGGAA GCTTTGTTGG CATCTGAAGA CTATGGGAAG
    GACTTGGCAT CAGTGAACAA CCTTCTGAAG AAGCACCAAT TACTGGAAGC TGATATATCT
    GCTCATGAGG ATCGGCTGAA GGACCTGAAC AGCCAGGCTG ACAGTTTGAT GACCAGCAGT
    GCTTTCGATA CCTCCCAAGT AAAGGATAAA CGTGAAACTA TAAATGGGCG CTTCCAGAGA
    ATCAAGAGCA TGGCAGCTGC CCGCCGTGCG AAGCTCAACG AGTCGCACCG CTTGCATCAG
    TTCTTCCGTG ACATGGATGA TGAGGAGTCC TGGATCAAAG AGAAGAAACT GTTGGTGAGC
    TCAGAGGACT ATGGCAGAGA CCTGACTGGT GTGCAGAACC TGAGGAAGAA ACATAAGCGC
    TTGGAAGCAG AATTAGCTGC CCATGAACCT GCTATCCAGG GTGTTCTAGA CACTGGCAAG
    AAGCTTTCAG ATGATAACAC AATTGGGAAG GAGGAGATAC AGCAAAGACT GGCTCAGTTT
    GTGGACCACT GGAAAGAGTT AAAACAGCTG GCAGCTGCTC GGGGGCAGCG TCTGGAGGAG
    TCCCTGGAGT ACCAACAGTT TGTAGCAAAT GTTGAGGAAG AGGAGGCATG GATCAACGAG
    AAAATGACAT TGGTAGCCAG TGAGGATTAT GGAGACACAC TTGCTGCTAT CCAGGGCTTG
    CTGAAGAAGC ATGAAGCATT CGAGACTGAC TTTACTGTCC ACAAAGACAG AGTGAATGAT
    GTTTGCGCTA ATGGAGAGGA TCTCATTAAA AAGAATAATC ACCATGTGGA GAACATTACT
    GCTAAGATGA AGGGCCTCAA GGGCAAGGTA TCAGATCTGG AGAAAGCCGC AGCTCAGAGG
    AAAGCCAAAC TGGATGAGAA CTCTGCCTTC CTCCAGTTCA ACTGGAAAGC AGATGTGGTG
    GAGTCATGGA TAGGTGAGAA GGAAAACAGT CTGAAGACAG ATGATTATGG CCGTGACCTC
    TCCTCTGTGC AAACACTACT CACCAAACAG GAAACCTTTG ATGCTGGACT TCAGGCTTTC
    CAGCAGGAGG GAATTGCAAA CATCACTGCT CTGAAAGACC AGCTACTGGC AGCCAAACAT
    ATCCAATCAA AGGCCATTGA GGTTCGTCAC GCTTCCTTGA TGAAACGCTG GAATCAGCTG
    CTTGCTAATT CTGCAGCCAG GAAAAAGAAA CTCTTGGAGG CTCAGGAGCA CTTCAGAAAG
    GTTGAAGATC TCTTCTTGAC TTTTGCCAAG AAGGCCTCTG CCTTCAACAG TTGGTTTGAG
    AATGCTGAGG AGGACCTGAC GGATCCCGTG CGCTGCAATT CCCTGGAAGA AATCAAAGCA
    CTGCGAGAAG CCCACGATGC GTTCCGTTCC TCACTTAGCT CTGCCCAAGC TGACTTCAAC
    CAGCTGGCAG AGCTTGATCG GCAGATCAAG AGCTTCCGTG TAGCCTCCAA CCCGTACACT
    TGGTTTACTA TGGAGGCTCT TGAAGAAACT TGGAGGAATC TGCAGAAAAT TATCAAGGAG
    CGTGAATTGG AGTTGCAGAA GGAGCAGCGG AGGCAGGAAG AAAATGACAA GTTGCGCCAG
    GAGTTTGCTC AGCATGCTAA TGCCTTCCAC CAGTGGATTC AGGAGACAAG GACTTACCTG
    CTAGATGGGT CCTGCATGGT GGAGGAGTCG GGAACACTGG AATCCCAGCT GGAAGCTACT
    AAACGCAAGC ACCAGGAGAT CCGGGCTATG AGGAGCCAGC TGAAGAAGAT TGAGGACCTT
    GGAGCAGCCA TGGAAGAGGC ACTTATCTTG GACAACAAAT ACACAGAGCA CAGCACCGTG
    GGGCTGGCGC AGCAGTGGGA CCAGCTTGAC CAGCTGGGAA TGAGAATGCA GCACAATCTG
    GAACAGCAGA TTCAAGCTCG AAACACAACT GGAGTCACAG AGGAGGCCCT GAAGGAGTTC
    AGCATGATGT TCAAGCACTT TGACAAGGAC AAATCTGGAC GTCTTAATCA CCAGGAGTTT
    AAGTCTTGCT TGCGCTCCCT TGGCTATGAC CTGCCCATGG TTGAGGAAGG AGAGCCTGAC
    CCCGAATTTG AGTCTATTCT TGACACTGTT GATCCCAACA GGGATGGCCA CGTCTCGCTG
    CAGGAGTACA TGGCGTTCAT GATCAGCAGG GAAACAGAGA ACGTGAAATC CAGCGAGGAG
    ATTGAGAGTG CTTTCCGTGC CCTCAGCTCG GAGGGGAAGC CTTACGTGAC CAAGGAGGAG
    CTCTACCAGA ACCTGACCCG GGAGCAGGCC GACTACTGCA TTTCTCACAT GAAGCCCTAC
    ATGGATGGCA AGGGCAGAGA ACTTCCTTCT GCCTACGACT ACATAGAATT TACACGTTCA
    CTCTTTGTGA ATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa43318
    Clone ID Related Accession (Same CDS sequence) XM_015279292.2 , XM_015279292.1
    Accession Version XM_015279292.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7455bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
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    1801
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    1981
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    3421
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    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
    TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG
    TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC
    TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT
    GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT
    GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG
    ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAA CTCGTCTACA GGAGCTGCAC
    CGACTATGGG AGTTACTGCT GGAAAAAATG AGAGAGAAGG GAGTGAAGCT GTTGCAAGCA
    CAGAAGCTGG TGCAATTCTT ACGGGAATGT GAAGATGTCA TGGACTGGAT CAATGACAAG
    GAAGCAATAG TGACCTCAGA AGAGCTTGGA CAGGACTTAG AGCATGTTGA GGTTTTGCAG
    AAGAAATTTG AAGAGTTCCA AACAGATCTG GCAGCTCATG AGGAGAGAGT GAATGAAGTG
    AACCAGTTCG CTGGCAAACT TATCCAGGAA CAGCACCCTG AGGAGGAACT TATAAAGTCC
    AAACAGGATG AAGTAAATGC AAGCTGGCAG CGTCTTAAGG GGCTTGCCCA ACAGAGGCAA
    GGAAAACTCT TTGGGGCAGC TGAAGTTCAG CGCTTCAACA GGGATGTGGA TGAAACTATC
    AGCTGGATTA AGGAGAAAGG GCAGTTGATG GCCTCAGATG ACTTTGGCAG AGACTTGGCC
    AGCGTGCAAG CTTTACTACG TAAGCATGAA GGCCTGGAAA GAGATCTTGC AGCTTTGGAG
    GATAAGGTTA AGGCCCTCTG TGCAGAAGCT GACCGTTTGC AGCAGTCTCA CCCTATAAAT
    GCTTCTCAAA TTCAAGTGAA ACGGGAGGAA CTGATTGCCA ACTGGGAGCA GATCCGAACT
    CTGGCAGCAG AGAGGCATGC TCGCCTTAAT GACTCCTACA GGTTGCAGCG CTTTCTTGCA
    GATTTCCGGG ACCTCACTAG CTGGGTAACT GAGATGAAGG CTCTGATAAA TGCTGATGAG
    CTTGCCAATG ATGTGGCTGG AGCAGAAGCC CTTCTAGATA GACATCAGGA ACATAAGGGA
    GAAATTGATG CTCATGAGGA TAGCTTCAGA TCTGCTGATG AGTCTGGGCA GGCTTTGCTT
    GCAGCTGGGC ACTATGCTTC TGATGAAGTT AAAGAAAAGC TGACTATCCT CTCAGATGAA
    AGATCAGCCT TGCTGGAACT ATGGGAGCTT CGCAGGCAAC AGTATGAGCA GTGCATGGAT
    CTGCAGCTTT TCTACAGAGA TACTGAACAA GTTGACAACT GGATGAGCAA ACAAGAAGCT
    TTTCTGCTGA ACGAAGACCT TGGTGATTCT CTGGATAGCG TGGAGGCTCT TCTAAAGAAG
    CATGAAGATT TTGAGAAATC CCTAAGTGCT CAAGAGGAGA AGATCACAGC ACTGGATGAG
    TTTGCTACTA AACTGATTCA AAATAACCAT TATGCCATGG ATGACGTTGC TACGCGCAGA
    GATGCTCTTC TGAGTCGCCG AAATGCTCTT CATGAAAGAG CCATGAAGCG CCGTGCCCAA
    CTGGCAGACT CTTTCCATCT CCAGCAGTTT TTCAGGGACT CTGATGAACT GAAGAGTTGG
    GTTAATGAAA AAATGAAAAC TGCTACCGAT GAGGCTTACA AGGATCCATC CAACTTGCAA
    GGTAAAGTTC AGAAGCATCA GGCTTTTGAA GCAGAGCTTT CTGCTAATCA GAGTCGTATT
    GATGCACTGG AGAAAGCTGG CCAGAAGTTG ATTGATGTCA ATCACTATGC ATCTGATGAG
    GTGGCAGCTC GCATGAATGA AGTCATCAGC TTGTGGAAGA AACTTCTGGA AGCCACCGAG
    CTCAAAGGTA TAAAGCTGCG TGAAGCCAAT CAACAGCAGC AATTTAATCG CAATGTAGAA
    GACATTGAGT TGTGGCTGTA TGAAGTGGAG GGTCACTTGG CTTCTGATGA TTATGGAAAA
    GATCTTACCA GCGTTCAGAA TCTTCAGAAG AAACATGCCC TGCTAGAGGC AGATGTTGCT
    GCCCATCAGG ATCGGATAGA TGGCATTACC ATCCAGGCAC GCCAGTTCCA AGATGCTGGG
    CACTTTGATG CTGACAATAT CAAGAAGAAA CAAGAAGCTT TAGTAGCTCG TTATGAAGCT
    CTGAAGGATC CTATGGTGGC TCGCAAGCAG AAACTTGCAG ATTCTCTTCG CCTGCAGCAG
    CTTTTCCGTG ACATCGAGGA TGAAGAGACC TGGATCAGGG AAAAGGAACC TATTGCAGCC
    TCAACAAACC GAGGCAAGGA CTTAATTGGT GTCCAGAATC TGCTAAAGAA GCACCAGGCT
    TTGCAGGCAG AAATTGCAGG CCATGAGCCT CGCATTAAAG CAGTCACACA AAAAGGAAAT
    GCGATGGTGG AAGAGGGACA TTTTGCTGCT GAAGATGTGA AGATCAAATT GAATGAACTA
    AACCAAAAAT GGGACTCTCT GAAAGCCAAG GCATCTCAAC GGCGACAGGA TCTAGAGGAT
    TCTCTGCAAG CTCAGCAGTA TTTTGCTGAT GCTAATGAGG CAGAATCATG GATGAGGGAA
    AAGGAGCCCA TTGTAGGCAG CACGGATTAT GGAAAGGATG AAGACTCTGC TGAGGCTCTC
    CTGAAGAAGC ATGAAGCTTT GATGTCTGAT CTTTCTGCTT ACGGCAGTAG CATACAGGCA
    TTAAGGGAAC AGGCCCAGTC CTGCAGGCAA CAAGTTGCTC CCACTGATGA TGAAACTGGA
    AAAGAGCTTG TGCTAGCACT CTATGATTAC CAAGAGAAGA GTCCTCGGGA GGTGACTATG
    AAGAAAGGAG ATATTCTAAC CCTGCTCAAC AGCACCAACA AGGACTGGTG GAAGGTTGAA
    GTTAACGATC GTCAGGGCTT TGTACCAGCT GCCTATGTGA AAAAACTAGA TCCTGCCCAG
    TCTGCATCCC GAGAGAATCT TCTAGAAGAG CAAGGCAGCA TAGCATTGCG ACAGGAGCAA
    ATTGACAACC AGACTCTCAT TACTAAGGAA GTCGGCAGTG TATCTCTGCG TATGAAACAG
    GTCGAAGAAC TGTATCATTC CCTTCTTGAG CTGGGAGAAA AACGTAAAGG CATGCTAGAA
    AAAAGCTGCA AGAAGTTTAT GCTTTTCCGT GAGGCTAATG AGCTTCAACA GTGGATCAAT
    GAGAAGGAAG CTGCGCTCAC TAATGAGGAA GTGGGTGCTG ATTTGGAGCA GGTGGAGGTG
    CTACAGAAGA AGTTTGATGA TTTTCAGAAG GATTTAAAAG CTAATGAGTC ACGACTGAAG
    GACATAAACA AGGTTGCAAA TGACCTGGAG TCTGAAGGGC TGATGGCAGA GGAAGTGCAA
    GCAGTAGAGC ACCAGGAAGT CTATGGAATG ATGCCCAGAG ATGAAACTGA TTCTAAGACA
    GCCTCTCCTT GGAAGTCTGC ACGTATGATG GTACACACAG TGGCAACGTT TAACTCAATC
    AAGGAACTGA ATGAACGCTG GAGATCCCTG CAGCAGTTGG CAGAGGAGCG AAGCCAGCTG
    TTGGGCAGCG CTCATGAAGT CCAGAGGTTC CACAGAGATG CTGATGAAAC AAAAGAATGG
    ATAGAAGAGA AGAATCAAGC ATTAAATACC GACAACTATG GACATGACTT GGCCAGTGTG
    CAGGCTCTGC AGCGCAAGCA TGAAGGCTTT GAGAGAGACT TGGCAGCTCT TGGAGACAAG
    GTGAATTCTC TTGGTGAAAC TGCCCAGCGT CTGATCCAGT CACATCCAGA ATCAGCTGAA
    GATCTTCAAG AAAAATGTAC TGAGTTGAAC CAAGCCTGGA GTAGTCTGGG AAAACGTGCT
    GACCAACGCA AAGAGAAGCT TGGAGATTCT CATGACCTGC AGCGTTTCCT CAGTGATTTT
    AGAGACCTTA TGTCTTGGAT CAATGGAATC CGGGGTCTGG TCTCCTCAGA TGAACTTGCA
    AAAGATGTGA CTGGAGCTGA AGCTCTGTTG GAAAGGCATC AGGAGCACCG TACTGAAATA
    GATGCACGGG CTGGCACTTT TCAGGCATTT GAACAGTTTG GACAGCAGCT TCTGGCTCGT
    GGACACTATG CCAGCCCAGA GATTAAGGAG AAACTGGATA TTCTAGATCA AGAACGGACA
    GACCTAGAGA AGGCCTGGGT CCAGCGCAGA ATGATGCTAG ACCAGTGCTT AGAACTACAG
    CTGTTTCATC GGGATTGTGA ACAAGCTGAA AACTGGATGG CTGCCCGAGA GGCGTTCCTA
    AATACAGAAG ACAAAGGAGA CTCCTTAGAC AGCGTGGAGG CACTCATCAA GAAGCATGAA
    GATTTCGATA AAGCAATCAA TGTCCAGGAA GAGAAAATTG CTGTCTTGCA GTCTTTTGCT
    GACCAACTGA TTGCTGCTGA TCATTATGCA AAAGGAGTCA TTGCTAACAG ACGCAATGAG
    GTTCTGGACA GGTGGCGTCG TCTGAAGGCT CAGATGATTG AGAAGAGATC TAAGCTGGGA
    GAATCTCAGA CCCTCCAACA GTTCAGTCGT GATGTTGATG AAATAGAAGC TTGGATCAGT
    GAAAAGCTTC AAACTGCAAG TGATGAGTCA TATAAGGATC CCACAAACAT CCAGAGCAAG
    CACCAGAAGC ACCAAGCCTT TGAAGCTGAG CTCCACGCCA ACGCAGATCG GATTCGTGGA
    GTCATTGAAA TGGGGAACTC TCTTATTGAA AGAGGAGCGT GTGCTGGCAG CGAGGATGCT
    GTGAAGGCTC GGCTGGCTGC CCTGGCTGAC CAATGGGAGT TCCTGGTCCA GAAGTCATCA
    GAGAAGAGTC AGAAACTGAA AGAAGCAAAT AAACAGCAGA ATTTCAATAC CGGCATCAAG
    GACTTTGATT TCTGGCTTTC AGAGGTGGAA GCTTTGTTGG CATCTGAAGA CTATGGGAAG
    GACTTGGCAT CAGTGAACAA CCTTCTGAAG AAGCACCAAT TACTGGAAGC TGATATATCT
    GCTCATGAGG ATCGGCTGAA GGACCTGAAC AGCCAGGCTG ACAGTTTGAT GACCAGCAGT
    GCTTTCGATA CCTCCCAAGT AAAGGATAAA CGTGAAACTA TAAATGGGCG CTTCCAGAGA
    ATCAAGAGCA TGGCAGCTGC CCGCCGTGCG AAGCTCAACG AGTCGCACCG CTTGCATCAG
    TTCTTCCGTG ACATGGATGA TGAGGAGTCC TGGATCAAAG AGAAGAAACT GTTGGTGAGC
    TCAGAGGACT ATGGCAGAGA CCTGACTGGT GTGCAGAACC TGAGGAAGAA ACATAAGCGC
    TTGGAAGCAG AATTAGCTGC CCATGAACCT GCTATCCAGG GTGTTCTAGA CACTGGCAAG
    AAGCTTTCAG ATGATAACAC AATTGGGAAG GAGGAGATAC AGCAAAGACT GGCTCAGTTT
    GTGGACCACT GGAAAGAGTT AAAACAGCTG GCAGCTGCTC GGGGGCAGCG TCTGGAGGAG
    TCCCTGGAGT ACCAACAGTT TGTAGCAAAT GTTGAGGAAG AGGAGGCATG GATCAACGAG
    AAAATGACAT TGGTAGCCAG TGAGGATTAT GGAGACACAC TTGCTGCTAT CCAGGGCTTG
    CTGAAGAAGC ATGAAGCATT CGAGACTGAC TTTACTGTCC ACAAAGACAG AGTGAATGAT
    GTTTGCGCTA ATGGAGAGGA TCTCATTAAA AAGAATAATC ACCATGTGGA GAACATTACT
    GCTAAGATGA AGGGCCTCAA GGGCAAGGTA TCAGATCTGG AGAAAGCCGC AGCTCAGAGG
    AAAGCCAAAC TGGATGAGAA CTCTGCCTTC CTCCAGTTCA ACTGGAAAGC AGATGTGGTG
    GAGTCATGGA TAGGTGAGAA GGAAAACAGT CTGAAGACAG ATGATTATGG CCGTGACCTC
    TCCTCTGTGC AAACACTACT CACCAAACAG GAAACCTTTG ATGCTGGACT TCAGGCTTTC
    CAGCAGGAGG GAATTGCAAA CATCACTGCT CTGAAAGACC AGCTACTGGC AGCCAAACAT
    ATCCAATCAA AGGCCATTGA GGTTCGTCAC GCTTCCTTGA TGAAACGCTG GAATCAGCTG
    CTTGCTAATT CTGCAGCCAG GAAAAAGAAA CTCTTGGAGG CTCAGGAGCA CTTCAGAAAG
    GTTGAAGATC TCTTCTTGAC TTTTGCCAAG AAGGCCTCTG CCTTCAACAG TTGGTTTGAG
    AATGCTGAGG AGGACCTGAC GGATCCCGTG CGCTGCAATT CCCTGGAAGA AATCAAAGCA
    CTGCGAGAAG CCCACGATGC GTTCCGTTCC TCACTTAGCT CTGCCCAAGC TGACTTCAAC
    CAGCTGGCAG AGCTTGATCG GCAGATCAAG AGCTTCCGTG TAGCCTCCAA CCCGTACACT
    TGGTTTACTA TGGAGGCTCT TGAAGAAACT TGGAGGAATC TGCAGAAAAT TATCAAGGAG
    CGTGAATTGG AGTTGCAGAA GGAGCAGCGG AGGCAGGAAG AAAATGACAA GTTGCGCCAG
    GAGTTTGCTC AGCATGCTAA TGCCTTCCAC CAGTGGATTC AGGAGACAAG GACTTACCTG
    CTAGATGGGT CCTGCATGGT GGAGGAGTCG GGAACACTGG AATCCCAGCT GGAAGCTACT
    AAACGCAAGC ACCAGGAGAT CCGGGCTATG AGGAGCCAGC TGAAGAAGAT TGAGGACCTT
    GGAGCAGCCA TGGAAGAGGC ACTTATCTTG GACAACAAAT ACACAGAGCA CAGCACCGTG
    GGGCTGGCGC AGCAGTGGGA CCAGCTTGAC CAGCTGGGAA TGAGAATGCA GCACAATCTG
    GAACAGCAGA TTCAAGCTCG AAACACAACT GGAGTCACAG AGGAGGCCCT GAAGGAGTTC
    AGCATGATGT TCAAGCACTT TGACAAGGAC AAATCTGGAC GTCTTAATCA CCAGGAGTTT
    AAGTCTTGCT TGCGCTCCCT TGGCTATGAC CTGCCCATGG TTGAGGAAGG AGAGCCTGAC
    CCCGAATTTG AGTCTATTCT TGACACTGTT GATCCCAACA GGGATGGCCA CGTCTCGCTG
    CAGGAGTACA TGGCGTTCAT GATCAGCAGG GAAACAGAGA ACGTGAAATC CAGCGAGGAG
    ATTGAGAGTG CTTTCCGTGC CCTCAGCTCG GAGGGGAAGC CTTACGTGAC CAAGGAGGAG
    CTCTACCAGA ACCTGACCCG GGAGCAGGCC GACTACTGCA TTTCTCACAT GAAGCCCTAC
    ATGGATGGCA AGGGCAGAGA ACTTCCTTCT GCCTACGACT ACATAGAATT TACACGTTCA
    CTCTTTGTGA ATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015134778.1
    CDS10..7464
    Translation

    Target ORF information:

    RefSeq Version XM_015279292.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015279292.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
    TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG
    TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC
    TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT
    GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT
    GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG
    ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAA CTCGTCTACA GGAGCTGCAC
    CGACTATGGG AGTTACTGCT GGAAAAAATG AGAGAGAAGG GAGTGAAGCT GTTGCAAGCA
    CAGAAGCTGG TGCAATTCTT ACGGGAATGT GAAGATGTCA TGGACTGGAT CAATGACAAG
    GAAGCAATAG TGACCTCAGA AGAGCTTGGA CAGGACTTAG AGCATGTTGA GGTTTTGCAG
    AAGAAATTTG AAGAGTTCCA AACAGATCTG GCAGCTCATG AGGAGAGAGT GAATGAAGTG
    AACCAGTTCG CTGGCAAACT TATCCAGGAA CAGCACCCTG AGGAGGAACT TATAAAGTCC
    AAACAGGATG AAGTAAATGC AAGCTGGCAG CGTCTTAAGG GGCTTGCCCA ACAGAGGCAA
    GGAAAACTCT TTGGGGCAGC TGAAGTTCAG CGCTTCAACA GGGATGTGGA TGAAACTATC
    AGCTGGATTA AGGAGAAAGG GCAGTTGATG GCCTCAGATG ACTTTGGCAG AGACTTGGCC
    AGCGTGCAAG CTTTACTACG TAAGCATGAA GGCCTGGAAA GAGATCTTGC AGCTTTGGAG
    GATAAGGTTA AGGCCCTCTG TGCAGAAGCT GACCGTTTGC AGCAGTCTCA CCCTATAAAT
    GCTTCTCAAA TTCAAGTGAA ACGGGAGGAA CTGATTGCCA ACTGGGAGCA GATCCGAACT
    CTGGCAGCAG AGAGGCATGC TCGCCTTAAT GACTCCTACA GGTTGCAGCG CTTTCTTGCA
    GATTTCCGGG ACCTCACTAG CTGGGTAACT GAGATGAAGG CTCTGATAAA TGCTGATGAG
    CTTGCCAATG ATGTGGCTGG AGCAGAAGCC CTTCTAGATA GACATCAGGA ACATAAGGGA
    GAAATTGATG CTCATGAGGA TAGCTTCAGA TCTGCTGATG AGTCTGGGCA GGCTTTGCTT
    GCAGCTGGGC ACTATGCTTC TGATGAAGTT AAAGAAAAGC TGACTATCCT CTCAGATGAA
    AGATCAGCCT TGCTGGAACT ATGGGAGCTT CGCAGGCAAC AGTATGAGCA GTGCATGGAT
    CTGCAGCTTT TCTACAGAGA TACTGAACAA GTTGACAACT GGATGAGCAA ACAAGAAGCT
    TTTCTGCTGA ACGAAGACCT TGGTGATTCT CTGGATAGCG TGGAGGCTCT TCTAAAGAAG
    CATGAAGATT TTGAGAAATC CCTAAGTGCT CAAGAGGAGA AGATCACAGC ACTGGATGAG
    TTTGCTACTA AACTGATTCA AAATAACCAT TATGCCATGG ATGACGTTGC TACGCGCAGA
    GATGCTCTTC TGAGTCGCCG AAATGCTCTT CATGAAAGAG CCATGAAGCG CCGTGCCCAA
    CTGGCAGACT CTTTCCATCT CCAGCAGTTT TTCAGGGACT CTGATGAACT GAAGAGTTGG
    GTTAATGAAA AAATGAAAAC TGCTACCGAT GAGGCTTACA AGGATCCATC CAACTTGCAA
    GGTAAAGTTC AGAAGCATCA GGCTTTTGAA GCAGAGCTTT CTGCTAATCA GAGTCGTATT
    GATGCACTGG AGAAAGCTGG CCAGAAGTTG ATTGATGTCA ATCACTATGC ATCTGATGAG
    GTGGCAGCTC GCATGAATGA AGTCATCAGC TTGTGGAAGA AACTTCTGGA AGCCACCGAG
    CTCAAAGGTA TAAAGCTGCG TGAAGCCAAT CAACAGCAGC AATTTAATCG CAATGTAGAA
    GACATTGAGT TGTGGCTGTA TGAAGTGGAG GGTCACTTGG CTTCTGATGA TTATGGAAAA
    GATCTTACCA GCGTTCAGAA TCTTCAGAAG AAACATGCCC TGCTAGAGGC AGATGTTGCT
    GCCCATCAGG ATCGGATAGA TGGCATTACC ATCCAGGCAC GCCAGTTCCA AGATGCTGGG
    CACTTTGATG CTGACAATAT CAAGAAGAAA CAAGAAGCTT TAGTAGCTCG TTATGAAGCT
    CTGAAGGATC CTATGGTGGC TCGCAAGCAG AAACTTGCAG ATTCTCTTCG CCTGCAGCAG
    CTTTTCCGTG ACATCGAGGA TGAAGAGACC TGGATCAGGG AAAAGGAACC TATTGCAGCC
    TCAACAAACC GAGGCAAGGA CTTAATTGGT GTCCAGAATC TGCTAAAGAA GCACCAGGCT
    TTGCAGGCAG AAATTGCAGG CCATGAGCCT CGCATTAAAG CAGTCACACA AAAAGGAAAT
    GCGATGGTGG AAGAGGGACA TTTTGCTGCT GAAGATGTGA AGATCAAATT GAATGAACTA
    AACCAAAAAT GGGACTCTCT GAAAGCCAAG GCATCTCAAC GGCGACAGGA TCTAGAGGAT
    TCTCTGCAAG CTCAGCAGTA TTTTGCTGAT GCTAATGAGG CAGAATCATG GATGAGGGAA
    AAGGAGCCCA TTGTAGGCAG CACGGATTAT GGAAAGGATG AAGACTCTGC TGAGGCTCTC
    CTGAAGAAGC ATGAAGCTTT GATGTCTGAT CTTTCTGCTT ACGGCAGTAG CATACAGGCA
    TTAAGGGAAC AGGCCCAGTC CTGCAGGCAA CAAGTTGCTC CCACTGATGA TGAAACTGGA
    AAAGAGCTTG TGCTAGCACT CTATGATTAC CAAGAGAAGA GTCCTCGGGA GGTGACTATG
    AAGAAAGGAG ATATTCTAAC CCTGCTCAAC AGCACCAACA AGGACTGGTG GAAGGTTGAA
    GTTAACGATC GTCAGGGCTT TGTACCAGCT GCCTATGTGA AAAAACTAGA TCCTGCCCAG
    TCTGCATCCC GAGAGAATCT TCTAGAAGAG CAAGGCAGCA TAGCATTGCG ACAGGAGCAA
    ATTGACAACC AGACTCTCAT TACTAAGGAA GTCGGCAGTG TATCTCTGCG TATGAAACAG
    GTCGAAGAAC TGTATCATTC CCTTCTTGAG CTGGGAGAAA AACGTAAAGG CATGCTAGAA
    AAAAGCTGCA AGAAGTTTAT GCTTTTCCGT GAGGCTAATG AGCTTCAACA GTGGATCAAT
    GAGAAGGAAG CTGCGCTCAC TAATGAGGAA GTGGGTGCTG ATTTGGAGCA GGTGGAGGTG
    CTACAGAAGA AGTTTGATGA TTTTCAGAAG GATTTAAAAG CTAATGAGTC ACGACTGAAG
    GACATAAACA AGGTTGCAAA TGACCTGGAG TCTGAAGGGC TGATGGCAGA GGAAGTGCAA
    GCAGTAGAGC ACCAGGAAGT CTATGGAATG ATGCCCAGAG ATGAAACTGA TTCTAAGACA
    GCCTCTCCTT GGAAGTCTGC ACGTATGATG GTACACACAG TGGCAACGTT TAACTCAATC
    AAGGAACTGA ATGAACGCTG GAGATCCCTG CAGCAGTTGG CAGAGGAGCG AAGCCAGCTG
    TTGGGCAGCG CTCATGAAGT CCAGAGGTTC CACAGAGATG CTGATGAAAC AAAAGAATGG
    ATAGAAGAGA AGAATCAAGC ATTAAATACC GACAACTATG GACATGACTT GGCCAGTGTG
    CAGGCTCTGC AGCGCAAGCA TGAAGGCTTT GAGAGAGACT TGGCAGCTCT TGGAGACAAG
    GTGAATTCTC TTGGTGAAAC TGCCCAGCGT CTGATCCAGT CACATCCAGA ATCAGCTGAA
    GATCTTCAAG AAAAATGTAC TGAGTTGAAC CAAGCCTGGA GTAGTCTGGG AAAACGTGCT
    GACCAACGCA AAGAGAAGCT TGGAGATTCT CATGACCTGC AGCGTTTCCT CAGTGATTTT
    AGAGACCTTA TGTCTTGGAT CAATGGAATC CGGGGTCTGG TCTCCTCAGA TGAACTTGCA
    AAAGATGTGA CTGGAGCTGA AGCTCTGTTG GAAAGGCATC AGGAGCACCG TACTGAAATA
    GATGCACGGG CTGGCACTTT TCAGGCATTT GAACAGTTTG GACAGCAGCT TCTGGCTCGT
    GGACACTATG CCAGCCCAGA GATTAAGGAG AAACTGGATA TTCTAGATCA AGAACGGACA
    GACCTAGAGA AGGCCTGGGT CCAGCGCAGA ATGATGCTAG ACCAGTGCTT AGAACTACAG
    CTGTTTCATC GGGATTGTGA ACAAGCTGAA AACTGGATGG CTGCCCGAGA GGCGTTCCTA
    AATACAGAAG ACAAAGGAGA CTCCTTAGAC AGCGTGGAGG CACTCATCAA GAAGCATGAA
    GATTTCGATA AAGCAATCAA TGTCCAGGAA GAGAAAATTG CTGTCTTGCA GTCTTTTGCT
    GACCAACTGA TTGCTGCTGA TCATTATGCA AAAGGAGTCA TTGCTAACAG ACGCAATGAG
    GTTCTGGACA GGTGGCGTCG TCTGAAGGCT CAGATGATTG AGAAGAGATC TAAGCTGGGA
    GAATCTCAGA CCCTCCAACA GTTCAGTCGT GATGTTGATG AAATAGAAGC TTGGATCAGT
    GAAAAGCTTC AAACTGCAAG TGATGAGTCA TATAAGGATC CCACAAACAT CCAGAGCAAG
    CACCAGAAGC ACCAAGCCTT TGAAGCTGAG CTCCACGCCA ACGCAGATCG GATTCGTGGA
    GTCATTGAAA TGGGGAACTC TCTTATTGAA AGAGGAGCGT GTGCTGGCAG CGAGGATGCT
    GTGAAGGCTC GGCTGGCTGC CCTGGCTGAC CAATGGGAGT TCCTGGTCCA GAAGTCATCA
    GAGAAGAGTC AGAAACTGAA AGAAGCAAAT AAACAGCAGA ATTTCAATAC CGGCATCAAG
    GACTTTGATT TCTGGCTTTC AGAGGTGGAA GCTTTGTTGG CATCTGAAGA CTATGGGAAG
    GACTTGGCAT CAGTGAACAA CCTTCTGAAG AAGCACCAAT TACTGGAAGC TGATATATCT
    GCTCATGAGG ATCGGCTGAA GGACCTGAAC AGCCAGGCTG ACAGTTTGAT GACCAGCAGT
    GCTTTCGATA CCTCCCAAGT AAAGGATAAA CGTGAAACTA TAAATGGGCG CTTCCAGAGA
    ATCAAGAGCA TGGCAGCTGC CCGCCGTGCG AAGCTCAACG AGTCGCACCG CTTGCATCAG
    TTCTTCCGTG ACATGGATGA TGAGGAGTCC TGGATCAAAG AGAAGAAACT GTTGGTGAGC
    TCAGAGGACT ATGGCAGAGA CCTGACTGGT GTGCAGAACC TGAGGAAGAA ACATAAGCGC
    TTGGAAGCAG AATTAGCTGC CCATGAACCT GCTATCCAGG GTGTTCTAGA CACTGGCAAG
    AAGCTTTCAG ATGATAACAC AATTGGGAAG GAGGAGATAC AGCAAAGACT GGCTCAGTTT
    GTGGACCACT GGAAAGAGTT AAAACAGCTG GCAGCTGCTC GGGGGCAGCG TCTGGAGGAG
    TCCCTGGAGT ACCAACAGTT TGTAGCAAAT GTTGAGGAAG AGGAGGCATG GATCAACGAG
    AAAATGACAT TGGTAGCCAG TGAGGATTAT GGAGACACAC TTGCTGCTAT CCAGGGCTTG
    CTGAAGAAGC ATGAAGCATT CGAGACTGAC TTTACTGTCC ACAAAGACAG AGTGAATGAT
    GTTTGCGCTA ATGGAGAGGA TCTCATTAAA AAGAATAATC ACCATGTGGA GAACATTACT
    GCTAAGATGA AGGGCCTCAA GGGCAAGGTA TCAGATCTGG AGAAAGCCGC AGCTCAGAGG
    AAAGCCAAAC TGGATGAGAA CTCTGCCTTC CTCCAGTTCA ACTGGAAAGC AGATGTGGTG
    GAGTCATGGA TAGGTGAGAA GGAAAACAGT CTGAAGACAG ATGATTATGG CCGTGACCTC
    TCCTCTGTGC AAACACTACT CACCAAACAG GAAACCTTTG ATGCTGGACT TCAGGCTTTC
    CAGCAGGAGG GAATTGCAAA CATCACTGCT CTGAAAGACC AGCTACTGGC AGCCAAACAT
    ATCCAATCAA AGGCCATTGA GGTTCGTCAC GCTTCCTTGA TGAAACGCTG GAATCAGCTG
    CTTGCTAATT CTGCAGCCAG GAAAAAGAAA CTCTTGGAGG CTCAGGAGCA CTTCAGAAAG
    GTTGAAGATC TCTTCTTGAC TTTTGCCAAG AAGGCCTCTG CCTTCAACAG TTGGTTTGAG
    AATGCTGAGG AGGACCTGAC GGATCCCGTG CGCTGCAATT CCCTGGAAGA AATCAAAGCA
    CTGCGAGAAG CCCACGATGC GTTCCGTTCC TCACTTAGCT CTGCCCAAGC TGACTTCAAC
    CAGCTGGCAG AGCTTGATCG GCAGATCAAG AGCTTCCGTG TAGCCTCCAA CCCGTACACT
    TGGTTTACTA TGGAGGCTCT TGAAGAAACT TGGAGGAATC TGCAGAAAAT TATCAAGGAG
    CGTGAATTGG AGTTGCAGAA GGAGCAGCGG AGGCAGGAAG AAAATGACAA GTTGCGCCAG
    GAGTTTGCTC AGCATGCTAA TGCCTTCCAC CAGTGGATTC AGGAGACAAG GACTTACCTG
    CTAGATGGGT CCTGCATGGT GGAGGAGTCG GGAACACTGG AATCCCAGCT GGAAGCTACT
    AAACGCAAGC ACCAGGAGAT CCGGGCTATG AGGAGCCAGC TGAAGAAGAT TGAGGACCTT
    GGAGCAGCCA TGGAAGAGGC ACTTATCTTG GACAACAAAT ACACAGAGCA CAGCACCGTG
    GGGCTGGCGC AGCAGTGGGA CCAGCTTGAC CAGCTGGGAA TGAGAATGCA GCACAATCTG
    GAACAGCAGA TTCAAGCTCG AAACACAACT GGAGTCACAG AGGAGGCCCT GAAGGAGTTC
    AGCATGATGT TCAAGCACTT TGACAAGGAC AAATCTGGAC GTCTTAATCA CCAGGAGTTT
    AAGTCTTGCT TGCGCTCCCT TGGCTATGAC CTGCCCATGG TTGAGGAAGG AGAGCCTGAC
    CCCGAATTTG AGTCTATTCT TGACACTGTT GATCCCAACA GGGATGGCCA CGTCTCGCTG
    CAGGAGTACA TGGCGTTCAT GATCAGCAGG GAAACAGAGA ACGTGAAATC CAGCGAGGAG
    ATTGAGAGTG CTTTCCGTGC CCTCAGCTCG GAGGGGAAGC CTTACGTGAC CAAGGAGGAG
    CTCTACCAGA ACCTGACCCG GGAGCAGGCC GACTACTGCA TTTCTCACAT GAAGCCCTAC
    ATGGATGGCA AGGGCAGAGA ACTTCCTTCT GCCTACGACT ACATAGAATT TACACGTTCA
    CTCTTTGTGA ATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa43319
    Clone ID Related Accession (Same CDS sequence) XM_015279293.1 , XM_015279293.2
    Accession Version XM_015279293.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7410bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
    TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG
    TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC
    TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT
    GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT
    GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG
    ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAA CTCGTCTACA GGAGCTGCAC
    CGACTATGGG AGTTACTGCT GGAAAAAATG AGAGAGAAGG GAGTGAAGCT GTTGCAAGCA
    CAGAAGCTGG TGCAATTCTT ACGGGAATGT GAAGATGTCA TGGACTGGAT CAATGACAAG
    GAAGCAATAG TGACCTCAGA AGAGCTTGGA CAGGACTTAG AGCATGTTGA GGTTTTGCAG
    AAGAAATTTG AAGAGTTCCA AACAGATCTG GCAGCTCATG AGGAGAGAGT GAATGAAGTG
    AACCAGTTCG CTGGCAAACT TATCCAGGAA CAGCACCCTG AGGAGGAACT TATAAAGTCC
    AAACAGGATG AAGTAAATGC AAGCTGGCAG CGTCTTAAGG GGCTTGCCCA ACAGAGGCAA
    GGAAAACTCT TTGGGGCAGC TGAAGTTCAG CGCTTCAACA GGGATGTGGA TGAAACTATC
    AGCTGGATTA AGGAGAAAGG GCAGTTGATG GCCTCAGATG ACTTTGGCAG AGACTTGGCC
    AGCGTGCAAG CTTTACTACG TAAGCATGAA GGCCTGGAAA GAGATCTTGC AGCTTTGGAG
    GATAAGGTTA AGGCCCTCTG TGCAGAAGCT GACCGTTTGC AGCAGTCTCA CCCTATAAAT
    GCTTCTCAAA TTCAAGTGAA ACGGGAGGAA CTGATTGCCA ACTGGGAGCA GATCCGAACT
    CTGGCAGCAG AGAGGCATGC TCGCCTTAAT GACTCCTACA GGTTGCAGCG CTTTCTTGCA
    GATTTCCGGG ACCTCACTAG CTGGGTAACT GAGATGAAGG CTCTGATAAA TGCTGATGAG
    CTTGCCAATG ATGTGGCTGG AGCAGAAGCC CTTCTAGATA GACATCAGGA ACATAAGGGA
    GAAATTGATG CTCATGAGGA TAGCTTCAGA TCTGCTGATG AGTCTGGGCA GGCTTTGCTT
    GCAGCTGGGC ACTATGCTTC TGATGAAGTT AAAGAAAAGC TGACTATCCT CTCAGATGAA
    AGATCAGCCT TGCTGGAACT ATGGGAGCTT CGCAGGCAAC AGTATGAGCA GTGCATGGAT
    CTGCAGCTTT TCTACAGAGA TACTGAACAA GTTGACAACT GGATGAGCAA ACAAGAAGCT
    TTTCTGCTGA ACGAAGACCT TGGTGATTCT CTGGATAGCG TGGAGGCTCT TCTAAAGAAG
    CATGAAGATT TTGAGAAATC CCTAAGTGCT CAAGAGGAGA AGATCACAGC ACTGGATGAG
    TTTGCTACTA AACTGATTCA AAATAACCAT TATGCCATGG ATGACGTTGC TACGCGCAGA
    GATGCTCTTC TGAGTCGCCG AAATGCTCTT CATGAAAGAG CCATGAAGCG CCGTGCCCAA
    CTGGCAGACT CTTTCCATCT CCAGCAGTTT TTCAGGGACT CTGATGAACT GAAGAGTTGG
    GTTAATGAAA AAATGAAAAC TGCTACCGAT GAGGCTTACA AGGATCCATC CAACTTGCAA
    GGTAAAGTTC AGAAGCATCA GGCTTTTGAA GCAGAGCTTT CTGCTAATCA GAGTCGTATT
    GATGCACTGG AGAAAGCTGG CCAGAAGTTG ATTGATGTCA ATCACTATGC ATCTGATGAG
    GTGGCAGCTC GCATGAATGA AGTCATCAGC TTGTGGAAGA AACTTCTGGA AGCCACCGAG
    CTCAAAGGTA TAAAGCTGCG TGAAGCCAAT CAACAGCAGC AATTTAATCG CAATGTAGAA
    GACATTGAGT TGTGGCTGTA TGAAGTGGAG GGTCACTTGG CTTCTGATGA TTATGGAAAA
    GATCTTACCA GCGTTCAGAA TCTTCAGAAG AAACATGCCC TGCTAGAGGC AGATGTTGCT
    GCCCATCAGG ATCGGATAGA TGGCATTACC ATCCAGGCAC GCCAGTTCCA AGATGCTGGG
    CACTTTGATG CTGACAATAT CAAGAAGAAA CAAGAAGCTT TAGTAGCTCG TTATGAAGCT
    CTGAAGGATC CTATGGTGGC TCGCAAGCAG AAACTTGCAG ATTCTCTTCG CCTGCAGCAG
    CTTTTCCGTG ACATCGAGGA TGAAGAGACC TGGATCAGGG AAAAGGAACC TATTGCAGCC
    TCAACAAACC GAGGCAAGGA CTTAATTGGT GTCCAGAATC TGCTAAAGAA GCACCAGGCT
    TTGCAGGCAG AAATTGCAGG CCATGAGCCT CGCATTAAAG CAGTCACACA AAAAGGAAAT
    GCGATGGTGG AAGAGGGACA TTTTGCTGCT GAAGATGTGA AGATCAAATT GAATGAACTA
    AACCAAAAAT GGGACTCTCT GAAAGCCAAG GCATCTCAAC GGCGACAGGA TCTAGAGGAT
    TCTCTGCAAG CTCAGCAGTA TTTTGCTGAT GCTAATGAGG CAGAATCATG GATGAGGGAA
    AAGGAGCCCA TTGTAGGCAG CACGGATTAT GGAAAGGATG AAGACTCTGC TGAGGCTCTC
    CTGAAGAAGC ATGAAGCTTT GATGTCTGAT CTTTCTGCTT ACGGCAGTAG CATACAGGCA
    TTAAGGGAAC AGGCCCAGTC CTGCAGGCAA CAAGTTGCTC CCACTGATGA TGAAACTGGA
    AAAGAGCTTG TGCTAGCACT CTATGATTAC CAAGAGAAGA GTCCTCGGGA GGTGACTATG
    AAGAAAGGAG ATATTCTAAC CCTGCTCAAC AGCACCAACA AGGACTGGTG GAAGGTTGAA
    GTTAACGATC GTCAGGGCTT TGTACCAGCT GCCTATGTGA AAAAACTAGA TCCTGCCCAG
    TCTGCATCCC GAGAGAATCT TCTAGAAGAG CAAGGCAGCA TAGCATTGCG ACAGGAGCAA
    ATTGACAACC AGTATCATTC CCTTCTTGAG CTGGGAGAAA AACGTAAAGG CATGCTAGAA
    AAAAGCTGCA AGAAGTTTAT GCTTTTCCGT GAGGCTAATG AGCTTCAACA GTGGATCAAT
    GAGAAGGAAG CTGCGCTCAC TAATGAGGAA GTGGGTGCTG ATTTGGAGCA GGTGGAGGTG
    CTACAGAAGA AGTTTGATGA TTTTCAGAAG GATTTAAAAG CTAATGAGTC ACGACTGAAG
    GACATAAACA AGGTTGCAAA TGACCTGGAG TCTGAAGGGC TGATGGCAGA GGAAGTGCAA
    GCAGTAGAGC ACCAGGAAGT CTATGGAATG ATGCCCAGAG ATGAAACTGA TTCTAAGACA
    GCCTCTCCTT GGAAGTCTGC ACGTATGATG GTACACACAG TGGCAACGTT TAACTCAATC
    AAGGAACTGA ATGAACGCTG GAGATCCCTG CAGCAGTTGG CAGAGGAGCG AAGCCAGCTG
    TTGGGCAGCG CTCATGAAGT CCAGAGGTTC CACAGAGATG CTGATGAAAC AAAAGAATGG
    ATAGAAGAGA AGAATCAAGC ATTAAATACC GACAACTATG GACATGACTT GGCCAGTGTG
    CAGGCTCTGC AGCGCAAGCA TGAAGGCTTT GAGAGAGACT TGGCAGCTCT TGGAGACAAG
    GTGAATTCTC TTGGTGAAAC TGCCCAGCGT CTGATCCAGT CACATCCAGA ATCAGCTGAA
    GATCTTCAAG AAAAATGTAC TGAGTTGAAC CAAGCCTGGA GTAGTCTGGG AAAACGTGCT
    GACCAACGCA AAGAGAAGCT TGGAGATTCT CATGACCTGC AGCGTTTCCT CAGTGATTTT
    AGAGACCTTA TGTCTTGGAT CAATGGAATC CGGGGTCTGG TCTCCTCAGA TGAACTTGCA
    AAAGATGTGA CTGGAGCTGA AGCTCTGTTG GAAAGGCATC AGGAGCACCG TACTGAAATA
    GATGCACGGG CTGGCACTTT TCAGGCATTT GAACAGTTTG GACAGCAGCT TCTGGCTCGT
    GGACACTATG CCAGCCCAGA GATTAAGGAG AAACTGGATA TTCTAGATCA AGAACGGACA
    GACCTAGAGA AGGCCTGGGT CCAGCGCAGA ATGATGCTAG ACCAGTGCTT AGAACTACAG
    CTGTTTCATC GGGATTGTGA ACAAGCTGAA AACTGGATGG CTGCCCGAGA GGCGTTCCTA
    AATACAGAAG ACAAAGGAGA CTCCTTAGAC AGCGTGGAGG CACTCATCAA GAAGCATGAA
    GATTTCGATA AAGCAATCAA TGTCCAGGAA GAGAAAATTG CTGTCTTGCA GTCTTTTGCT
    GACCAACTGA TTGCTGCTGA TCATTATGCA AAAGGAGTCA TTGCTAACAG ACGCAATGAG
    GTTCTGGACA GGTGGCGTCG TCTGAAGGCT CAGATGATTG AGAAGAGATC TAAGCTGGGA
    GAATCTCAGA CCCTCCAACA GTTCAGTCGT GATGTTGATG AAATAGAAGC TTGGATCAGT
    GAAAAGCTTC AAACTGCAAG TGATGAGTCA TATAAGGATC CCACAAACAT CCAGCTTTCC
    AAACTGCTGA GCAAGCACCA GAAGCACCAA GCCTTTGAAG CTGAGCTCCA CGCCAACGCA
    GATCGGATTC GTGGAGTCAT TGAAATGGGG AACTCTCTTA TTGAAAGAGG AGCGTGTGCT
    GGCAGCGAGG ATGCTGTGAA GGCTCGGCTG GCTGCCCTGG CTGACCAATG GGAGTTCCTG
    GTCCAGAAGT CATCAGAGAA GAGTCAGAAA CTGAAAGAAG CAAATAAACA GCAGAATTTC
    AATACCGGCA TCAAGGACTT TGATTTCTGG CTTTCAGAGG TGGAAGCTTT GTTGGCATCT
    GAAGACTATG GGAAGGACTT GGCATCAGTG AACAACCTTC TGAAGAAGCA CCAATTACTG
    GAAGCTGATA TATCTGCTCA TGAGGATCGG CTGAAGGACC TGAACAGCCA GGCTGACAGT
    TTGATGACCA GCAGTGCTTT CGATACCTCC CAAGTAAAGG ATAAACGTGA AACTATAAAT
    GGGCGCTTCC AGAGAATCAA GAGCATGGCA GCTGCCCGCC GTGCGAAGCT CAACGAGTCG
    CACCGCTTGC ATCAGTTCTT CCGTGACATG GATGATGAGG AGTCCTGGAT CAAAGAGAAG
    AAACTGTTGG TGAGCTCAGA GGACTATGGC AGAGACCTGA CTGGTGTGCA GAACCTGAGG
    AAGAAACATA AGCGCTTGGA AGCAGAATTA GCTGCCCATG AACCTGCTAT CCAGGGTGTT
    CTAGACACTG GCAAGAAGCT TTCAGATGAT AACACAATTG GGAAGGAGGA GATACAGCAA
    AGACTGGCTC AGTTTGTGGA CCACTGGAAA GAGTTAAAAC AGCTGGCAGC TGCTCGGGGG
    CAGCGTCTGG AGGAGTCCCT GGAGTACCAA CAGTTTGTAG CAAATGTTGA GGAAGAGGAG
    GCATGGATCA ACGAGAAAAT GACATTGGTA GCCAGTGAGG ATTATGGAGA CACACTTGCT
    GCTATCCAGG GCTTGCTGAA GAAGCATGAA GCATTCGAGA CTGACTTTAC TGTCCACAAA
    GACAGAGTGA ATGATGTTTG CGCTAATGGA GAGGATCTCA TTAAAAAGAA TAATCACCAT
    GTGGAGAACA TTACTGCTAA GATGAAGGGC CTCAAGGGCA AGGTATCAGA TCTGGAGAAA
    GCCGCAGCTC AGAGGAAAGC CAAACTGGAT GAGAACTCTG CCTTCCTCCA GTTCAACTGG
    AAAGCAGATG TGGTGGAGTC ATGGATAGGT GAGAAGGAAA ACAGTCTGAA GACAGATGAT
    TATGGCCGTG ACCTCTCCTC TGTGCAAACA CTACTCACCA AACAGGAAAC CTTTGATGCT
    GGACTTCAGG CTTTCCAGCA GGAGGGAATT GCAAACATCA CTGCTCTGAA AGACCAGCTA
    CTGGCAGCCA AACATATCCA ATCAAAGGCC ATTGAGGTTC GTCACGCTTC CTTGATGAAA
    CGCTGGAATC AGCTGCTTGC TAATTCTGCA GCCAGGAAAA AGAAACTCTT GGAGGCTCAG
    GAGCACTTCA GAAAGGTTGA AGATCTCTTC TTGACTTTTG CCAAGAAGGC CTCTGCCTTC
    AACAGTTGGT TTGAGAATGC TGAGGAGGAC CTGACGGATC CCGTGCGCTG CAATTCCCTG
    GAAGAAATCA AAGCACTGCG AGAAGCCCAC GATGCGTTCC GTTCCTCACT TAGCTCTGCC
    CAAGCTGACT TCAACCAGCT GGCAGAGCTT GATCGGCAGA TCAAGAGCTT CCGTGTAGCC
    TCCAACCCGT ACACTTGGTT TACTATGGAG GCTCTTGAAG AAACTTGGAG GAATCTGCAG
    AAAATTATCA AGGAGCGTGA ATTGGAGTTG CAGAAGGAGC AGCGGAGGCA GGAAGAAAAT
    GACAAGTTGC GCCAGGAGTT TGCTCAGCAT GCTAATGCCT TCCACCAGTG GATTCAGGAG
    ACAAGGACTT ACCTGCTAGA TGGGTCCTGC ATGGTGGAGG AGTCGGGAAC ACTGGAATCC
    CAGCTGGAAG CTACTAAACG CAAGCACCAG GAGATCCGGG CTATGAGGAG CCAGCTGAAG
    AAGATTGAGG ACCTTGGAGC AGCCATGGAA GAGGCACTTA TCTTGGACAA CAAATACACA
    GAGCACAGCA CCGTGGGGCT GGCGCAGCAG TGGGACCAGC TTGACCAGCT GGGAATGAGA
    ATGCAGCACA ATCTGGAACA GCAGATTCAA GCTCGAAACA CAACTGGAGT CACAGAGGAG
    GCCCTGAAGG AGTTCAGCAT GATGTTCAAG CACTTTGACA AGGACAAATC TGGACGTCTT
    AATCACCAGG AGTTTAAGTC TTGCTTGCGC TCCCTTGGCT ATGACCTGCC CATGGTTGAG
    GAAGGAGAGC CTGACCCCGA ATTTGAGTCT ATTCTTGACA CTGTTGATCC CAACAGGGAT
    GGCCACGTCT CGCTGCAGGA GTACATGGCG TTCATGATCA GCAGGGAAAC AGAGAACGTG
    AAATCCAGCG AGGAGATTGA GAGTGCTTTC CGTGCCCTCA GCTCGGAGGG GAAGCCTTAC
    GTGACCAAGG AGGAGCTCTA CCAGAACCTG ACCCGGGAGC AGGCCGACTA CTGCATTTCT
    CACATGAAGC CCTACATGGA TGGCAAGGGC AGAGAACTTC CTTCTGCCTA CGACTACATA
    GAATTTACAC GTTCACTCTT TGTGAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015134779.1
    CDS10..7419
    Translation

    Target ORF information:

    RefSeq Version XM_015279293.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015279293.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
    TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG
    TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC
    TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT
    GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT
    GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG
    ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAA CTCGTCTACA GGAGCTGCAC
    CGACTATGGG AGTTACTGCT GGAAAAAATG AGAGAGAAGG GAGTGAAGCT GTTGCAAGCA
    CAGAAGCTGG TGCAATTCTT ACGGGAATGT GAAGATGTCA TGGACTGGAT CAATGACAAG
    GAAGCAATAG TGACCTCAGA AGAGCTTGGA CAGGACTTAG AGCATGTTGA GGTTTTGCAG
    AAGAAATTTG AAGAGTTCCA AACAGATCTG GCAGCTCATG AGGAGAGAGT GAATGAAGTG
    AACCAGTTCG CTGGCAAACT TATCCAGGAA CAGCACCCTG AGGAGGAACT TATAAAGTCC
    AAACAGGATG AAGTAAATGC AAGCTGGCAG CGTCTTAAGG GGCTTGCCCA ACAGAGGCAA
    GGAAAACTCT TTGGGGCAGC TGAAGTTCAG CGCTTCAACA GGGATGTGGA TGAAACTATC
    AGCTGGATTA AGGAGAAAGG GCAGTTGATG GCCTCAGATG ACTTTGGCAG AGACTTGGCC
    AGCGTGCAAG CTTTACTACG TAAGCATGAA GGCCTGGAAA GAGATCTTGC AGCTTTGGAG
    GATAAGGTTA AGGCCCTCTG TGCAGAAGCT GACCGTTTGC AGCAGTCTCA CCCTATAAAT
    GCTTCTCAAA TTCAAGTGAA ACGGGAGGAA CTGATTGCCA ACTGGGAGCA GATCCGAACT
    CTGGCAGCAG AGAGGCATGC TCGCCTTAAT GACTCCTACA GGTTGCAGCG CTTTCTTGCA
    GATTTCCGGG ACCTCACTAG CTGGGTAACT GAGATGAAGG CTCTGATAAA TGCTGATGAG
    CTTGCCAATG ATGTGGCTGG AGCAGAAGCC CTTCTAGATA GACATCAGGA ACATAAGGGA
    GAAATTGATG CTCATGAGGA TAGCTTCAGA TCTGCTGATG AGTCTGGGCA GGCTTTGCTT
    GCAGCTGGGC ACTATGCTTC TGATGAAGTT AAAGAAAAGC TGACTATCCT CTCAGATGAA
    AGATCAGCCT TGCTGGAACT ATGGGAGCTT CGCAGGCAAC AGTATGAGCA GTGCATGGAT
    CTGCAGCTTT TCTACAGAGA TACTGAACAA GTTGACAACT GGATGAGCAA ACAAGAAGCT
    TTTCTGCTGA ACGAAGACCT TGGTGATTCT CTGGATAGCG TGGAGGCTCT TCTAAAGAAG
    CATGAAGATT TTGAGAAATC CCTAAGTGCT CAAGAGGAGA AGATCACAGC ACTGGATGAG
    TTTGCTACTA AACTGATTCA AAATAACCAT TATGCCATGG ATGACGTTGC TACGCGCAGA
    GATGCTCTTC TGAGTCGCCG AAATGCTCTT CATGAAAGAG CCATGAAGCG CCGTGCCCAA
    CTGGCAGACT CTTTCCATCT CCAGCAGTTT TTCAGGGACT CTGATGAACT GAAGAGTTGG
    GTTAATGAAA AAATGAAAAC TGCTACCGAT GAGGCTTACA AGGATCCATC CAACTTGCAA
    GGTAAAGTTC AGAAGCATCA GGCTTTTGAA GCAGAGCTTT CTGCTAATCA GAGTCGTATT
    GATGCACTGG AGAAAGCTGG CCAGAAGTTG ATTGATGTCA ATCACTATGC ATCTGATGAG
    GTGGCAGCTC GCATGAATGA AGTCATCAGC TTGTGGAAGA AACTTCTGGA AGCCACCGAG
    CTCAAAGGTA TAAAGCTGCG TGAAGCCAAT CAACAGCAGC AATTTAATCG CAATGTAGAA
    GACATTGAGT TGTGGCTGTA TGAAGTGGAG GGTCACTTGG CTTCTGATGA TTATGGAAAA
    GATCTTACCA GCGTTCAGAA TCTTCAGAAG AAACATGCCC TGCTAGAGGC AGATGTTGCT
    GCCCATCAGG ATCGGATAGA TGGCATTACC ATCCAGGCAC GCCAGTTCCA AGATGCTGGG
    CACTTTGATG CTGACAATAT CAAGAAGAAA CAAGAAGCTT TAGTAGCTCG TTATGAAGCT
    CTGAAGGATC CTATGGTGGC TCGCAAGCAG AAACTTGCAG ATTCTCTTCG CCTGCAGCAG
    CTTTTCCGTG ACATCGAGGA TGAAGAGACC TGGATCAGGG AAAAGGAACC TATTGCAGCC
    TCAACAAACC GAGGCAAGGA CTTAATTGGT GTCCAGAATC TGCTAAAGAA GCACCAGGCT
    TTGCAGGCAG AAATTGCAGG CCATGAGCCT CGCATTAAAG CAGTCACACA AAAAGGAAAT
    GCGATGGTGG AAGAGGGACA TTTTGCTGCT GAAGATGTGA AGATCAAATT GAATGAACTA
    AACCAAAAAT GGGACTCTCT GAAAGCCAAG GCATCTCAAC GGCGACAGGA TCTAGAGGAT
    TCTCTGCAAG CTCAGCAGTA TTTTGCTGAT GCTAATGAGG CAGAATCATG GATGAGGGAA
    AAGGAGCCCA TTGTAGGCAG CACGGATTAT GGAAAGGATG AAGACTCTGC TGAGGCTCTC
    CTGAAGAAGC ATGAAGCTTT GATGTCTGAT CTTTCTGCTT ACGGCAGTAG CATACAGGCA
    TTAAGGGAAC AGGCCCAGTC CTGCAGGCAA CAAGTTGCTC CCACTGATGA TGAAACTGGA
    AAAGAGCTTG TGCTAGCACT CTATGATTAC CAAGAGAAGA GTCCTCGGGA GGTGACTATG
    AAGAAAGGAG ATATTCTAAC CCTGCTCAAC AGCACCAACA AGGACTGGTG GAAGGTTGAA
    GTTAACGATC GTCAGGGCTT TGTACCAGCT GCCTATGTGA AAAAACTAGA TCCTGCCCAG
    TCTGCATCCC GAGAGAATCT TCTAGAAGAG CAAGGCAGCA TAGCATTGCG ACAGGAGCAA
    ATTGACAACC AGTATCATTC CCTTCTTGAG CTGGGAGAAA AACGTAAAGG CATGCTAGAA
    AAAAGCTGCA AGAAGTTTAT GCTTTTCCGT GAGGCTAATG AGCTTCAACA GTGGATCAAT
    GAGAAGGAAG CTGCGCTCAC TAATGAGGAA GTGGGTGCTG ATTTGGAGCA GGTGGAGGTG
    CTACAGAAGA AGTTTGATGA TTTTCAGAAG GATTTAAAAG CTAATGAGTC ACGACTGAAG
    GACATAAACA AGGTTGCAAA TGACCTGGAG TCTGAAGGGC TGATGGCAGA GGAAGTGCAA
    GCAGTAGAGC ACCAGGAAGT CTATGGAATG ATGCCCAGAG ATGAAACTGA TTCTAAGACA
    GCCTCTCCTT GGAAGTCTGC ACGTATGATG GTACACACAG TGGCAACGTT TAACTCAATC
    AAGGAACTGA ATGAACGCTG GAGATCCCTG CAGCAGTTGG CAGAGGAGCG AAGCCAGCTG
    TTGGGCAGCG CTCATGAAGT CCAGAGGTTC CACAGAGATG CTGATGAAAC AAAAGAATGG
    ATAGAAGAGA AGAATCAAGC ATTAAATACC GACAACTATG GACATGACTT GGCCAGTGTG
    CAGGCTCTGC AGCGCAAGCA TGAAGGCTTT GAGAGAGACT TGGCAGCTCT TGGAGACAAG
    GTGAATTCTC TTGGTGAAAC TGCCCAGCGT CTGATCCAGT CACATCCAGA ATCAGCTGAA
    GATCTTCAAG AAAAATGTAC TGAGTTGAAC CAAGCCTGGA GTAGTCTGGG AAAACGTGCT
    GACCAACGCA AAGAGAAGCT TGGAGATTCT CATGACCTGC AGCGTTTCCT CAGTGATTTT
    AGAGACCTTA TGTCTTGGAT CAATGGAATC CGGGGTCTGG TCTCCTCAGA TGAACTTGCA
    AAAGATGTGA CTGGAGCTGA AGCTCTGTTG GAAAGGCATC AGGAGCACCG TACTGAAATA
    GATGCACGGG CTGGCACTTT TCAGGCATTT GAACAGTTTG GACAGCAGCT TCTGGCTCGT
    GGACACTATG CCAGCCCAGA GATTAAGGAG AAACTGGATA TTCTAGATCA AGAACGGACA
    GACCTAGAGA AGGCCTGGGT CCAGCGCAGA ATGATGCTAG ACCAGTGCTT AGAACTACAG
    CTGTTTCATC GGGATTGTGA ACAAGCTGAA AACTGGATGG CTGCCCGAGA GGCGTTCCTA
    AATACAGAAG ACAAAGGAGA CTCCTTAGAC AGCGTGGAGG CACTCATCAA GAAGCATGAA
    GATTTCGATA AAGCAATCAA TGTCCAGGAA GAGAAAATTG CTGTCTTGCA GTCTTTTGCT
    GACCAACTGA TTGCTGCTGA TCATTATGCA AAAGGAGTCA TTGCTAACAG ACGCAATGAG
    GTTCTGGACA GGTGGCGTCG TCTGAAGGCT CAGATGATTG AGAAGAGATC TAAGCTGGGA
    GAATCTCAGA CCCTCCAACA GTTCAGTCGT GATGTTGATG AAATAGAAGC TTGGATCAGT
    GAAAAGCTTC AAACTGCAAG TGATGAGTCA TATAAGGATC CCACAAACAT CCAGCTTTCC
    AAACTGCTGA GCAAGCACCA GAAGCACCAA GCCTTTGAAG CTGAGCTCCA CGCCAACGCA
    GATCGGATTC GTGGAGTCAT TGAAATGGGG AACTCTCTTA TTGAAAGAGG AGCGTGTGCT
    GGCAGCGAGG ATGCTGTGAA GGCTCGGCTG GCTGCCCTGG CTGACCAATG GGAGTTCCTG
    GTCCAGAAGT CATCAGAGAA GAGTCAGAAA CTGAAAGAAG CAAATAAACA GCAGAATTTC
    AATACCGGCA TCAAGGACTT TGATTTCTGG CTTTCAGAGG TGGAAGCTTT GTTGGCATCT
    GAAGACTATG GGAAGGACTT GGCATCAGTG AACAACCTTC TGAAGAAGCA CCAATTACTG
    GAAGCTGATA TATCTGCTCA TGAGGATCGG CTGAAGGACC TGAACAGCCA GGCTGACAGT
    TTGATGACCA GCAGTGCTTT CGATACCTCC CAAGTAAAGG ATAAACGTGA AACTATAAAT
    GGGCGCTTCC AGAGAATCAA GAGCATGGCA GCTGCCCGCC GTGCGAAGCT CAACGAGTCG
    CACCGCTTGC ATCAGTTCTT CCGTGACATG GATGATGAGG AGTCCTGGAT CAAAGAGAAG
    AAACTGTTGG TGAGCTCAGA GGACTATGGC AGAGACCTGA CTGGTGTGCA GAACCTGAGG
    AAGAAACATA AGCGCTTGGA AGCAGAATTA GCTGCCCATG AACCTGCTAT CCAGGGTGTT
    CTAGACACTG GCAAGAAGCT TTCAGATGAT AACACAATTG GGAAGGAGGA GATACAGCAA
    AGACTGGCTC AGTTTGTGGA CCACTGGAAA GAGTTAAAAC AGCTGGCAGC TGCTCGGGGG
    CAGCGTCTGG AGGAGTCCCT GGAGTACCAA CAGTTTGTAG CAAATGTTGA GGAAGAGGAG
    GCATGGATCA ACGAGAAAAT GACATTGGTA GCCAGTGAGG ATTATGGAGA CACACTTGCT
    GCTATCCAGG GCTTGCTGAA GAAGCATGAA GCATTCGAGA CTGACTTTAC TGTCCACAAA
    GACAGAGTGA ATGATGTTTG CGCTAATGGA GAGGATCTCA TTAAAAAGAA TAATCACCAT
    GTGGAGAACA TTACTGCTAA GATGAAGGGC CTCAAGGGCA AGGTATCAGA TCTGGAGAAA
    GCCGCAGCTC AGAGGAAAGC CAAACTGGAT GAGAACTCTG CCTTCCTCCA GTTCAACTGG
    AAAGCAGATG TGGTGGAGTC ATGGATAGGT GAGAAGGAAA ACAGTCTGAA GACAGATGAT
    TATGGCCGTG ACCTCTCCTC TGTGCAAACA CTACTCACCA AACAGGAAAC CTTTGATGCT
    GGACTTCAGG CTTTCCAGCA GGAGGGAATT GCAAACATCA CTGCTCTGAA AGACCAGCTA
    CTGGCAGCCA AACATATCCA ATCAAAGGCC ATTGAGGTTC GTCACGCTTC CTTGATGAAA
    CGCTGGAATC AGCTGCTTGC TAATTCTGCA GCCAGGAAAA AGAAACTCTT GGAGGCTCAG
    GAGCACTTCA GAAAGGTTGA AGATCTCTTC TTGACTTTTG CCAAGAAGGC CTCTGCCTTC
    AACAGTTGGT TTGAGAATGC TGAGGAGGAC CTGACGGATC CCGTGCGCTG CAATTCCCTG
    GAAGAAATCA AAGCACTGCG AGAAGCCCAC GATGCGTTCC GTTCCTCACT TAGCTCTGCC
    CAAGCTGACT TCAACCAGCT GGCAGAGCTT GATCGGCAGA TCAAGAGCTT CCGTGTAGCC
    TCCAACCCGT ACACTTGGTT TACTATGGAG GCTCTTGAAG AAACTTGGAG GAATCTGCAG
    AAAATTATCA AGGAGCGTGA ATTGGAGTTG CAGAAGGAGC AGCGGAGGCA GGAAGAAAAT
    GACAAGTTGC GCCAGGAGTT TGCTCAGCAT GCTAATGCCT TCCACCAGTG GATTCAGGAG
    ACAAGGACTT ACCTGCTAGA TGGGTCCTGC ATGGTGGAGG AGTCGGGAAC ACTGGAATCC
    CAGCTGGAAG CTACTAAACG CAAGCACCAG GAGATCCGGG CTATGAGGAG CCAGCTGAAG
    AAGATTGAGG ACCTTGGAGC AGCCATGGAA GAGGCACTTA TCTTGGACAA CAAATACACA
    GAGCACAGCA CCGTGGGGCT GGCGCAGCAG TGGGACCAGC TTGACCAGCT GGGAATGAGA
    ATGCAGCACA ATCTGGAACA GCAGATTCAA GCTCGAAACA CAACTGGAGT CACAGAGGAG
    GCCCTGAAGG AGTTCAGCAT GATGTTCAAG CACTTTGACA AGGACAAATC TGGACGTCTT
    AATCACCAGG AGTTTAAGTC TTGCTTGCGC TCCCTTGGCT ATGACCTGCC CATGGTTGAG
    GAAGGAGAGC CTGACCCCGA ATTTGAGTCT ATTCTTGACA CTGTTGATCC CAACAGGGAT
    GGCCACGTCT CGCTGCAGGA GTACATGGCG TTCATGATCA GCAGGGAAAC AGAGAACGTG
    AAATCCAGCG AGGAGATTGA GAGTGCTTTC CGTGCCCTCA GCTCGGAGGG GAAGCCTTAC
    GTGACCAAGG AGGAGCTCTA CCAGAACCTG ACCCGGGAGC AGGCCGACTA CTGCATTTCT
    CACATGAAGC CCTACATGGA TGGCAAGGGC AGAGAACTTC CTTCTGCCTA CGACTACATA
    GAATTTACAC GTTCACTCTT TGTGAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa43319
    Clone ID Related Accession (Same CDS sequence) XM_015279293.1 , XM_015279293.2
    Accession Version XM_015279293.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7410bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015279293.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
    TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG
    TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC
    TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT
    GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT
    GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG
    ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAA CTCGTCTACA GGAGCTGCAC
    CGACTATGGG AGTTACTGCT GGAAAAAATG AGAGAGAAGG GAGTGAAGCT GTTGCAAGCA
    CAGAAGCTGG TGCAATTCTT ACGGGAATGT GAAGATGTCA TGGACTGGAT CAATGACAAG
    GAAGCAATAG TGACCTCAGA AGAGCTTGGA CAGGACTTAG AGCATGTTGA GGTTTTGCAG
    AAGAAATTTG AAGAGTTCCA AACAGATCTG GCAGCTCATG AGGAGAGAGT GAATGAAGTG
    AACCAGTTCG CTGGCAAACT TATCCAGGAA CAGCACCCTG AGGAGGAACT TATAAAGTCC
    AAACAGGATG AAGTAAATGC AAGCTGGCAG CGTCTTAAGG GGCTTGCCCA ACAGAGGCAA
    GGAAAACTCT TTGGGGCAGC TGAAGTTCAG CGCTTCAACA GGGATGTGGA TGAAACTATC
    AGCTGGATTA AGGAGAAAGG GCAGTTGATG GCCTCAGATG ACTTTGGCAG AGACTTGGCC
    AGCGTGCAAG CTTTACTACG TAAGCATGAA GGCCTGGAAA GAGATCTTGC AGCTTTGGAG
    GATAAGGTTA AGGCCCTCTG TGCAGAAGCT GACCGTTTGC AGCAGTCTCA CCCTATAAAT
    GCTTCTCAAA TTCAAGTGAA ACGGGAGGAA CTGATTGCCA ACTGGGAGCA GATCCGAACT
    CTGGCAGCAG AGAGGCATGC TCGCCTTAAT GACTCCTACA GGTTGCAGCG CTTTCTTGCA
    GATTTCCGGG ACCTCACTAG CTGGGTAACT GAGATGAAGG CTCTGATAAA TGCTGATGAG
    CTTGCCAATG ATGTGGCTGG AGCAGAAGCC CTTCTAGATA GACATCAGGA ACATAAGGGA
    GAAATTGATG CTCATGAGGA TAGCTTCAGA TCTGCTGATG AGTCTGGGCA GGCTTTGCTT
    GCAGCTGGGC ACTATGCTTC TGATGAAGTT AAAGAAAAGC TGACTATCCT CTCAGATGAA
    AGATCAGCCT TGCTGGAACT ATGGGAGCTT CGCAGGCAAC AGTATGAGCA GTGCATGGAT
    CTGCAGCTTT TCTACAGAGA TACTGAACAA GTTGACAACT GGATGAGCAA ACAAGAAGCT
    TTTCTGCTGA ACGAAGACCT TGGTGATTCT CTGGATAGCG TGGAGGCTCT TCTAAAGAAG
    CATGAAGATT TTGAGAAATC CCTAAGTGCT CAAGAGGAGA AGATCACAGC ACTGGATGAG
    TTTGCTACTA AACTGATTCA AAATAACCAT TATGCCATGG ATGACGTTGC TACGCGCAGA
    GATGCTCTTC TGAGTCGCCG AAATGCTCTT CATGAAAGAG CCATGAAGCG CCGTGCCCAA
    CTGGCAGACT CTTTCCATCT CCAGCAGTTT TTCAGGGACT CTGATGAACT GAAGAGTTGG
    GTTAATGAAA AAATGAAAAC TGCTACCGAT GAGGCTTACA AGGATCCATC CAACTTGCAA
    GGTAAAGTTC AGAAGCATCA GGCTTTTGAA GCAGAGCTTT CTGCTAATCA GAGTCGTATT
    GATGCACTGG AGAAAGCTGG CCAGAAGTTG ATTGATGTCA ATCACTATGC ATCTGATGAG
    GTGGCAGCTC GCATGAATGA AGTCATCAGC TTGTGGAAGA AACTTCTGGA AGCCACCGAG
    CTCAAAGGTA TAAAGCTGCG TGAAGCCAAT CAACAGCAGC AATTTAATCG CAATGTAGAA
    GACATTGAGT TGTGGCTGTA TGAAGTGGAG GGTCACTTGG CTTCTGATGA TTATGGAAAA
    GATCTTACCA GCGTTCAGAA TCTTCAGAAG AAACATGCCC TGCTAGAGGC AGATGTTGCT
    GCCCATCAGG ATCGGATAGA TGGCATTACC ATCCAGGCAC GCCAGTTCCA AGATGCTGGG
    CACTTTGATG CTGACAATAT CAAGAAGAAA CAAGAAGCTT TAGTAGCTCG TTATGAAGCT
    CTGAAGGATC CTATGGTGGC TCGCAAGCAG AAACTTGCAG ATTCTCTTCG CCTGCAGCAG
    CTTTTCCGTG ACATCGAGGA TGAAGAGACC TGGATCAGGG AAAAGGAACC TATTGCAGCC
    TCAACAAACC GAGGCAAGGA CTTAATTGGT GTCCAGAATC TGCTAAAGAA GCACCAGGCT
    TTGCAGGCAG AAATTGCAGG CCATGAGCCT CGCATTAAAG CAGTCACACA AAAAGGAAAT
    GCGATGGTGG AAGAGGGACA TTTTGCTGCT GAAGATGTGA AGATCAAATT GAATGAACTA
    AACCAAAAAT GGGACTCTCT GAAAGCCAAG GCATCTCAAC GGCGACAGGA TCTAGAGGAT
    TCTCTGCAAG CTCAGCAGTA TTTTGCTGAT GCTAATGAGG CAGAATCATG GATGAGGGAA
    AAGGAGCCCA TTGTAGGCAG CACGGATTAT GGAAAGGATG AAGACTCTGC TGAGGCTCTC
    CTGAAGAAGC ATGAAGCTTT GATGTCTGAT CTTTCTGCTT ACGGCAGTAG CATACAGGCA
    TTAAGGGAAC AGGCCCAGTC CTGCAGGCAA CAAGTTGCTC CCACTGATGA TGAAACTGGA
    AAAGAGCTTG TGCTAGCACT CTATGATTAC CAAGAGAAGA GTCCTCGGGA GGTGACTATG
    AAGAAAGGAG ATATTCTAAC CCTGCTCAAC AGCACCAACA AGGACTGGTG GAAGGTTGAA
    GTTAACGATC GTCAGGGCTT TGTACCAGCT GCCTATGTGA AAAAACTAGA TCCTGCCCAG
    TCTGCATCCC GAGAGAATCT TCTAGAAGAG CAAGGCAGCA TAGCATTGCG ACAGGAGCAA
    ATTGACAACC AGTATCATTC CCTTCTTGAG CTGGGAGAAA AACGTAAAGG CATGCTAGAA
    AAAAGCTGCA AGAAGTTTAT GCTTTTCCGT GAGGCTAATG AGCTTCAACA GTGGATCAAT
    GAGAAGGAAG CTGCGCTCAC TAATGAGGAA GTGGGTGCTG ATTTGGAGCA GGTGGAGGTG
    CTACAGAAGA AGTTTGATGA TTTTCAGAAG GATTTAAAAG CTAATGAGTC ACGACTGAAG
    GACATAAACA AGGTTGCAAA TGACCTGGAG TCTGAAGGGC TGATGGCAGA GGAAGTGCAA
    GCAGTAGAGC ACCAGGAAGT CTATGGAATG ATGCCCAGAG ATGAAACTGA TTCTAAGACA
    GCCTCTCCTT GGAAGTCTGC ACGTATGATG GTACACACAG TGGCAACGTT TAACTCAATC
    AAGGAACTGA ATGAACGCTG GAGATCCCTG CAGCAGTTGG CAGAGGAGCG AAGCCAGCTG
    TTGGGCAGCG CTCATGAAGT CCAGAGGTTC CACAGAGATG CTGATGAAAC AAAAGAATGG
    ATAGAAGAGA AGAATCAAGC ATTAAATACC GACAACTATG GACATGACTT GGCCAGTGTG
    CAGGCTCTGC AGCGCAAGCA TGAAGGCTTT GAGAGAGACT TGGCAGCTCT TGGAGACAAG
    GTGAATTCTC TTGGTGAAAC TGCCCAGCGT CTGATCCAGT CACATCCAGA ATCAGCTGAA
    GATCTTCAAG AAAAATGTAC TGAGTTGAAC CAAGCCTGGA GTAGTCTGGG AAAACGTGCT
    GACCAACGCA AAGAGAAGCT TGGAGATTCT CATGACCTGC AGCGTTTCCT CAGTGATTTT
    AGAGACCTTA TGTCTTGGAT CAATGGAATC CGGGGTCTGG TCTCCTCAGA TGAACTTGCA
    AAAGATGTGA CTGGAGCTGA AGCTCTGTTG GAAAGGCATC AGGAGCACCG TACTGAAATA
    GATGCACGGG CTGGCACTTT TCAGGCATTT GAACAGTTTG GACAGCAGCT TCTGGCTCGT
    GGACACTATG CCAGCCCAGA GATTAAGGAG AAACTGGATA TTCTAGATCA AGAACGGACA
    GACCTAGAGA AGGCCTGGGT CCAGCGCAGA ATGATGCTAG ACCAGTGCTT AGAACTACAG
    CTGTTTCATC GGGATTGTGA ACAAGCTGAA AACTGGATGG CTGCCCGAGA GGCGTTCCTA
    AATACAGAAG ACAAAGGAGA CTCCTTAGAC AGCGTGGAGG CACTCATCAA GAAGCATGAA
    GATTTCGATA AAGCAATCAA TGTCCAGGAA GAGAAAATTG CTGTCTTGCA GTCTTTTGCT
    GACCAACTGA TTGCTGCTGA TCATTATGCA AAAGGAGTCA TTGCTAACAG ACGCAATGAG
    GTTCTGGACA GGTGGCGTCG TCTGAAGGCT CAGATGATTG AGAAGAGATC TAAGCTGGGA
    GAATCTCAGA CCCTCCAACA GTTCAGTCGT GATGTTGATG AAATAGAAGC TTGGATCAGT
    GAAAAGCTTC AAACTGCAAG TGATGAGTCA TATAAGGATC CCACAAACAT CCAGCTTTCC
    AAACTGCTGA GCAAGCACCA GAAGCACCAA GCCTTTGAAG CTGAGCTCCA CGCCAACGCA
    GATCGGATTC GTGGAGTCAT TGAAATGGGG AACTCTCTTA TTGAAAGAGG AGCGTGTGCT
    GGCAGCGAGG ATGCTGTGAA GGCTCGGCTG GCTGCCCTGG CTGACCAATG GGAGTTCCTG
    GTCCAGAAGT CATCAGAGAA GAGTCAGAAA CTGAAAGAAG CAAATAAACA GCAGAATTTC
    AATACCGGCA TCAAGGACTT TGATTTCTGG CTTTCAGAGG TGGAAGCTTT GTTGGCATCT
    GAAGACTATG GGAAGGACTT GGCATCAGTG AACAACCTTC TGAAGAAGCA CCAATTACTG
    GAAGCTGATA TATCTGCTCA TGAGGATCGG CTGAAGGACC TGAACAGCCA GGCTGACAGT
    TTGATGACCA GCAGTGCTTT CGATACCTCC CAAGTAAAGG ATAAACGTGA AACTATAAAT
    GGGCGCTTCC AGAGAATCAA GAGCATGGCA GCTGCCCGCC GTGCGAAGCT CAACGAGTCG
    CACCGCTTGC ATCAGTTCTT CCGTGACATG GATGATGAGG AGTCCTGGAT CAAAGAGAAG
    AAACTGTTGG TGAGCTCAGA GGACTATGGC AGAGACCTGA CTGGTGTGCA GAACCTGAGG
    AAGAAACATA AGCGCTTGGA AGCAGAATTA GCTGCCCATG AACCTGCTAT CCAGGGTGTT
    CTAGACACTG GCAAGAAGCT TTCAGATGAT AACACAATTG GGAAGGAGGA GATACAGCAA
    AGACTGGCTC AGTTTGTGGA CCACTGGAAA GAGTTAAAAC AGCTGGCAGC TGCTCGGGGG
    CAGCGTCTGG AGGAGTCCCT GGAGTACCAA CAGTTTGTAG CAAATGTTGA GGAAGAGGAG
    GCATGGATCA ACGAGAAAAT GACATTGGTA GCCAGTGAGG ATTATGGAGA CACACTTGCT
    GCTATCCAGG GCTTGCTGAA GAAGCATGAA GCATTCGAGA CTGACTTTAC TGTCCACAAA
    GACAGAGTGA ATGATGTTTG CGCTAATGGA GAGGATCTCA TTAAAAAGAA TAATCACCAT
    GTGGAGAACA TTACTGCTAA GATGAAGGGC CTCAAGGGCA AGGTATCAGA TCTGGAGAAA
    GCCGCAGCTC AGAGGAAAGC CAAACTGGAT GAGAACTCTG CCTTCCTCCA GTTCAACTGG
    AAAGCAGATG TGGTGGAGTC ATGGATAGGT GAGAAGGAAA ACAGTCTGAA GACAGATGAT
    TATGGCCGTG ACCTCTCCTC TGTGCAAACA CTACTCACCA AACAGGAAAC CTTTGATGCT
    GGACTTCAGG CTTTCCAGCA GGAGGGAATT GCAAACATCA CTGCTCTGAA AGACCAGCTA
    CTGGCAGCCA AACATATCCA ATCAAAGGCC ATTGAGGTTC GTCACGCTTC CTTGATGAAA
    CGCTGGAATC AGCTGCTTGC TAATTCTGCA GCCAGGAAAA AGAAACTCTT GGAGGCTCAG
    GAGCACTTCA GAAAGGTTGA AGATCTCTTC TTGACTTTTG CCAAGAAGGC CTCTGCCTTC
    AACAGTTGGT TTGAGAATGC TGAGGAGGAC CTGACGGATC CCGTGCGCTG CAATTCCCTG
    GAAGAAATCA AAGCACTGCG AGAAGCCCAC GATGCGTTCC GTTCCTCACT TAGCTCTGCC
    CAAGCTGACT TCAACCAGCT GGCAGAGCTT GATCGGCAGA TCAAGAGCTT CCGTGTAGCC
    TCCAACCCGT ACACTTGGTT TACTATGGAG GCTCTTGAAG AAACTTGGAG GAATCTGCAG
    AAAATTATCA AGGAGCGTGA ATTGGAGTTG CAGAAGGAGC AGCGGAGGCA GGAAGAAAAT
    GACAAGTTGC GCCAGGAGTT TGCTCAGCAT GCTAATGCCT TCCACCAGTG GATTCAGGAG
    ACAAGGACTT ACCTGCTAGA TGGGTCCTGC ATGGTGGAGG AGTCGGGAAC ACTGGAATCC
    CAGCTGGAAG CTACTAAACG CAAGCACCAG GAGATCCGGG CTATGAGGAG CCAGCTGAAG
    AAGATTGAGG ACCTTGGAGC AGCCATGGAA GAGGCACTTA TCTTGGACAA CAAATACACA
    GAGCACAGCA CCGTGGGGCT GGCGCAGCAG TGGGACCAGC TTGACCAGCT GGGAATGAGA
    ATGCAGCACA ATCTGGAACA GCAGATTCAA GCTCGAAACA CAACTGGAGT CACAGAGGAG
    GCCCTGAAGG AGTTCAGCAT GATGTTCAAG CACTTTGACA AGGACAAATC TGGACGTCTT
    AATCACCAGG AGTTTAAGTC TTGCTTGCGC TCCCTTGGCT ATGACCTGCC CATGGTTGAG
    GAAGGAGAGC CTGACCCCGA ATTTGAGTCT ATTCTTGACA CTGTTGATCC CAACAGGGAT
    GGCCACGTCT CGCTGCAGGA GTACATGGCG TTCATGATCA GCAGGGAAAC AGAGAACGTG
    AAATCCAGCG AGGAGATTGA GAGTGCTTTC CGTGCCCTCA GCTCGGAGGG GAAGCCTTAC
    GTGACCAAGG AGGAGCTCTA CCAGAACCTG ACCCGGGAGC AGGCCGACTA CTGCATTTCT
    CACATGAAGC CCTACATGGA TGGCAAGGGC AGAGAACTTC CTTCTGCCTA CGACTACATA
    GAATTTACAC GTTCACTCTT TGTGAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015134779.1
    CDS7..7416
    Translation

    Target ORF information:

    RefSeq Version XM_015279293.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015279293.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
    TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG
    TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC
    TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT
    GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT
    GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG
    ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAA CTCGTCTACA GGAGCTGCAC
    CGACTATGGG AGTTACTGCT GGAAAAAATG AGAGAGAAGG GAGTGAAGCT GTTGCAAGCA
    CAGAAGCTGG TGCAATTCTT ACGGGAATGT GAAGATGTCA TGGACTGGAT CAATGACAAG
    GAAGCAATAG TGACCTCAGA AGAGCTTGGA CAGGACTTAG AGCATGTTGA GGTTTTGCAG
    AAGAAATTTG AAGAGTTCCA AACAGATCTG GCAGCTCATG AGGAGAGAGT GAATGAAGTG
    AACCAGTTCG CTGGCAAACT TATCCAGGAA CAGCACCCTG AGGAGGAACT TATAAAGTCC
    AAACAGGATG AAGTAAATGC AAGCTGGCAG CGTCTTAAGG GGCTTGCCCA ACAGAGGCAA
    GGAAAACTCT TTGGGGCAGC TGAAGTTCAG CGCTTCAACA GGGATGTGGA TGAAACTATC
    AGCTGGATTA AGGAGAAAGG GCAGTTGATG GCCTCAGATG ACTTTGGCAG AGACTTGGCC
    AGCGTGCAAG CTTTACTACG TAAGCATGAA GGCCTGGAAA GAGATCTTGC AGCTTTGGAG
    GATAAGGTTA AGGCCCTCTG TGCAGAAGCT GACCGTTTGC AGCAGTCTCA CCCTATAAAT
    GCTTCTCAAA TTCAAGTGAA ACGGGAGGAA CTGATTGCCA ACTGGGAGCA GATCCGAACT
    CTGGCAGCAG AGAGGCATGC TCGCCTTAAT GACTCCTACA GGTTGCAGCG CTTTCTTGCA
    GATTTCCGGG ACCTCACTAG CTGGGTAACT GAGATGAAGG CTCTGATAAA TGCTGATGAG
    CTTGCCAATG ATGTGGCTGG AGCAGAAGCC CTTCTAGATA GACATCAGGA ACATAAGGGA
    GAAATTGATG CTCATGAGGA TAGCTTCAGA TCTGCTGATG AGTCTGGGCA GGCTTTGCTT
    GCAGCTGGGC ACTATGCTTC TGATGAAGTT AAAGAAAAGC TGACTATCCT CTCAGATGAA
    AGATCAGCCT TGCTGGAACT ATGGGAGCTT CGCAGGCAAC AGTATGAGCA GTGCATGGAT
    CTGCAGCTTT TCTACAGAGA TACTGAACAA GTTGACAACT GGATGAGCAA ACAAGAAGCT
    TTTCTGCTGA ACGAAGACCT TGGTGATTCT CTGGATAGCG TGGAGGCTCT TCTAAAGAAG
    CATGAAGATT TTGAGAAATC CCTAAGTGCT CAAGAGGAGA AGATCACAGC ACTGGATGAG
    TTTGCTACTA AACTGATTCA AAATAACCAT TATGCCATGG ATGACGTTGC TACGCGCAGA
    GATGCTCTTC TGAGTCGCCG AAATGCTCTT CATGAAAGAG CCATGAAGCG CCGTGCCCAA
    CTGGCAGACT CTTTCCATCT CCAGCAGTTT TTCAGGGACT CTGATGAACT GAAGAGTTGG
    GTTAATGAAA AAATGAAAAC TGCTACCGAT GAGGCTTACA AGGATCCATC CAACTTGCAA
    GGTAAAGTTC AGAAGCATCA GGCTTTTGAA GCAGAGCTTT CTGCTAATCA GAGTCGTATT
    GATGCACTGG AGAAAGCTGG CCAGAAGTTG ATTGATGTCA ATCACTATGC ATCTGATGAG
    GTGGCAGCTC GCATGAATGA AGTCATCAGC TTGTGGAAGA AACTTCTGGA AGCCACCGAG
    CTCAAAGGTA TAAAGCTGCG TGAAGCCAAT CAACAGCAGC AATTTAATCG CAATGTAGAA
    GACATTGAGT TGTGGCTGTA TGAAGTGGAG GGTCACTTGG CTTCTGATGA TTATGGAAAA
    GATCTTACCA GCGTTCAGAA TCTTCAGAAG AAACATGCCC TGCTAGAGGC AGATGTTGCT
    GCCCATCAGG ATCGGATAGA TGGCATTACC ATCCAGGCAC GCCAGTTCCA AGATGCTGGG
    CACTTTGATG CTGACAATAT CAAGAAGAAA CAAGAAGCTT TAGTAGCTCG TTATGAAGCT
    CTGAAGGATC CTATGGTGGC TCGCAAGCAG AAACTTGCAG ATTCTCTTCG CCTGCAGCAG
    CTTTTCCGTG ACATCGAGGA TGAAGAGACC TGGATCAGGG AAAAGGAACC TATTGCAGCC
    TCAACAAACC GAGGCAAGGA CTTAATTGGT GTCCAGAATC TGCTAAAGAA GCACCAGGCT
    TTGCAGGCAG AAATTGCAGG CCATGAGCCT CGCATTAAAG CAGTCACACA AAAAGGAAAT
    GCGATGGTGG AAGAGGGACA TTTTGCTGCT GAAGATGTGA AGATCAAATT GAATGAACTA
    AACCAAAAAT GGGACTCTCT GAAAGCCAAG GCATCTCAAC GGCGACAGGA TCTAGAGGAT
    TCTCTGCAAG CTCAGCAGTA TTTTGCTGAT GCTAATGAGG CAGAATCATG GATGAGGGAA
    AAGGAGCCCA TTGTAGGCAG CACGGATTAT GGAAAGGATG AAGACTCTGC TGAGGCTCTC
    CTGAAGAAGC ATGAAGCTTT GATGTCTGAT CTTTCTGCTT ACGGCAGTAG CATACAGGCA
    TTAAGGGAAC AGGCCCAGTC CTGCAGGCAA CAAGTTGCTC CCACTGATGA TGAAACTGGA
    AAAGAGCTTG TGCTAGCACT CTATGATTAC CAAGAGAAGA GTCCTCGGGA GGTGACTATG
    AAGAAAGGAG ATATTCTAAC CCTGCTCAAC AGCACCAACA AGGACTGGTG GAAGGTTGAA
    GTTAACGATC GTCAGGGCTT TGTACCAGCT GCCTATGTGA AAAAACTAGA TCCTGCCCAG
    TCTGCATCCC GAGAGAATCT TCTAGAAGAG CAAGGCAGCA TAGCATTGCG ACAGGAGCAA
    ATTGACAACC AGTATCATTC CCTTCTTGAG CTGGGAGAAA AACGTAAAGG CATGCTAGAA
    AAAAGCTGCA AGAAGTTTAT GCTTTTCCGT GAGGCTAATG AGCTTCAACA GTGGATCAAT
    GAGAAGGAAG CTGCGCTCAC TAATGAGGAA GTGGGTGCTG ATTTGGAGCA GGTGGAGGTG
    CTACAGAAGA AGTTTGATGA TTTTCAGAAG GATTTAAAAG CTAATGAGTC ACGACTGAAG
    GACATAAACA AGGTTGCAAA TGACCTGGAG TCTGAAGGGC TGATGGCAGA GGAAGTGCAA
    GCAGTAGAGC ACCAGGAAGT CTATGGAATG ATGCCCAGAG ATGAAACTGA TTCTAAGACA
    GCCTCTCCTT GGAAGTCTGC ACGTATGATG GTACACACAG TGGCAACGTT TAACTCAATC
    AAGGAACTGA ATGAACGCTG GAGATCCCTG CAGCAGTTGG CAGAGGAGCG AAGCCAGCTG
    TTGGGCAGCG CTCATGAAGT CCAGAGGTTC CACAGAGATG CTGATGAAAC AAAAGAATGG
    ATAGAAGAGA AGAATCAAGC ATTAAATACC GACAACTATG GACATGACTT GGCCAGTGTG
    CAGGCTCTGC AGCGCAAGCA TGAAGGCTTT GAGAGAGACT TGGCAGCTCT TGGAGACAAG
    GTGAATTCTC TTGGTGAAAC TGCCCAGCGT CTGATCCAGT CACATCCAGA ATCAGCTGAA
    GATCTTCAAG AAAAATGTAC TGAGTTGAAC CAAGCCTGGA GTAGTCTGGG AAAACGTGCT
    GACCAACGCA AAGAGAAGCT TGGAGATTCT CATGACCTGC AGCGTTTCCT CAGTGATTTT
    AGAGACCTTA TGTCTTGGAT CAATGGAATC CGGGGTCTGG TCTCCTCAGA TGAACTTGCA
    AAAGATGTGA CTGGAGCTGA AGCTCTGTTG GAAAGGCATC AGGAGCACCG TACTGAAATA
    GATGCACGGG CTGGCACTTT TCAGGCATTT GAACAGTTTG GACAGCAGCT TCTGGCTCGT
    GGACACTATG CCAGCCCAGA GATTAAGGAG AAACTGGATA TTCTAGATCA AGAACGGACA
    GACCTAGAGA AGGCCTGGGT CCAGCGCAGA ATGATGCTAG ACCAGTGCTT AGAACTACAG
    CTGTTTCATC GGGATTGTGA ACAAGCTGAA AACTGGATGG CTGCCCGAGA GGCGTTCCTA
    AATACAGAAG ACAAAGGAGA CTCCTTAGAC AGCGTGGAGG CACTCATCAA GAAGCATGAA
    GATTTCGATA AAGCAATCAA TGTCCAGGAA GAGAAAATTG CTGTCTTGCA GTCTTTTGCT
    GACCAACTGA TTGCTGCTGA TCATTATGCA AAAGGAGTCA TTGCTAACAG ACGCAATGAG
    GTTCTGGACA GGTGGCGTCG TCTGAAGGCT CAGATGATTG AGAAGAGATC TAAGCTGGGA
    GAATCTCAGA CCCTCCAACA GTTCAGTCGT GATGTTGATG AAATAGAAGC TTGGATCAGT
    GAAAAGCTTC AAACTGCAAG TGATGAGTCA TATAAGGATC CCACAAACAT CCAGCTTTCC
    AAACTGCTGA GCAAGCACCA GAAGCACCAA GCCTTTGAAG CTGAGCTCCA CGCCAACGCA
    GATCGGATTC GTGGAGTCAT TGAAATGGGG AACTCTCTTA TTGAAAGAGG AGCGTGTGCT
    GGCAGCGAGG ATGCTGTGAA GGCTCGGCTG GCTGCCCTGG CTGACCAATG GGAGTTCCTG
    GTCCAGAAGT CATCAGAGAA GAGTCAGAAA CTGAAAGAAG CAAATAAACA GCAGAATTTC
    AATACCGGCA TCAAGGACTT TGATTTCTGG CTTTCAGAGG TGGAAGCTTT GTTGGCATCT
    GAAGACTATG GGAAGGACTT GGCATCAGTG AACAACCTTC TGAAGAAGCA CCAATTACTG
    GAAGCTGATA TATCTGCTCA TGAGGATCGG CTGAAGGACC TGAACAGCCA GGCTGACAGT
    TTGATGACCA GCAGTGCTTT CGATACCTCC CAAGTAAAGG ATAAACGTGA AACTATAAAT
    GGGCGCTTCC AGAGAATCAA GAGCATGGCA GCTGCCCGCC GTGCGAAGCT CAACGAGTCG
    CACCGCTTGC ATCAGTTCTT CCGTGACATG GATGATGAGG AGTCCTGGAT CAAAGAGAAG
    AAACTGTTGG TGAGCTCAGA GGACTATGGC AGAGACCTGA CTGGTGTGCA GAACCTGAGG
    AAGAAACATA AGCGCTTGGA AGCAGAATTA GCTGCCCATG AACCTGCTAT CCAGGGTGTT
    CTAGACACTG GCAAGAAGCT TTCAGATGAT AACACAATTG GGAAGGAGGA GATACAGCAA
    AGACTGGCTC AGTTTGTGGA CCACTGGAAA GAGTTAAAAC AGCTGGCAGC TGCTCGGGGG
    CAGCGTCTGG AGGAGTCCCT GGAGTACCAA CAGTTTGTAG CAAATGTTGA GGAAGAGGAG
    GCATGGATCA ACGAGAAAAT GACATTGGTA GCCAGTGAGG ATTATGGAGA CACACTTGCT
    GCTATCCAGG GCTTGCTGAA GAAGCATGAA GCATTCGAGA CTGACTTTAC TGTCCACAAA
    GACAGAGTGA ATGATGTTTG CGCTAATGGA GAGGATCTCA TTAAAAAGAA TAATCACCAT
    GTGGAGAACA TTACTGCTAA GATGAAGGGC CTCAAGGGCA AGGTATCAGA TCTGGAGAAA
    GCCGCAGCTC AGAGGAAAGC CAAACTGGAT GAGAACTCTG CCTTCCTCCA GTTCAACTGG
    AAAGCAGATG TGGTGGAGTC ATGGATAGGT GAGAAGGAAA ACAGTCTGAA GACAGATGAT
    TATGGCCGTG ACCTCTCCTC TGTGCAAACA CTACTCACCA AACAGGAAAC CTTTGATGCT
    GGACTTCAGG CTTTCCAGCA GGAGGGAATT GCAAACATCA CTGCTCTGAA AGACCAGCTA
    CTGGCAGCCA AACATATCCA ATCAAAGGCC ATTGAGGTTC GTCACGCTTC CTTGATGAAA
    CGCTGGAATC AGCTGCTTGC TAATTCTGCA GCCAGGAAAA AGAAACTCTT GGAGGCTCAG
    GAGCACTTCA GAAAGGTTGA AGATCTCTTC TTGACTTTTG CCAAGAAGGC CTCTGCCTTC
    AACAGTTGGT TTGAGAATGC TGAGGAGGAC CTGACGGATC CCGTGCGCTG CAATTCCCTG
    GAAGAAATCA AAGCACTGCG AGAAGCCCAC GATGCGTTCC GTTCCTCACT TAGCTCTGCC
    CAAGCTGACT TCAACCAGCT GGCAGAGCTT GATCGGCAGA TCAAGAGCTT CCGTGTAGCC
    TCCAACCCGT ACACTTGGTT TACTATGGAG GCTCTTGAAG AAACTTGGAG GAATCTGCAG
    AAAATTATCA AGGAGCGTGA ATTGGAGTTG CAGAAGGAGC AGCGGAGGCA GGAAGAAAAT
    GACAAGTTGC GCCAGGAGTT TGCTCAGCAT GCTAATGCCT TCCACCAGTG GATTCAGGAG
    ACAAGGACTT ACCTGCTAGA TGGGTCCTGC ATGGTGGAGG AGTCGGGAAC ACTGGAATCC
    CAGCTGGAAG CTACTAAACG CAAGCACCAG GAGATCCGGG CTATGAGGAG CCAGCTGAAG
    AAGATTGAGG ACCTTGGAGC AGCCATGGAA GAGGCACTTA TCTTGGACAA CAAATACACA
    GAGCACAGCA CCGTGGGGCT GGCGCAGCAG TGGGACCAGC TTGACCAGCT GGGAATGAGA
    ATGCAGCACA ATCTGGAACA GCAGATTCAA GCTCGAAACA CAACTGGAGT CACAGAGGAG
    GCCCTGAAGG AGTTCAGCAT GATGTTCAAG CACTTTGACA AGGACAAATC TGGACGTCTT
    AATCACCAGG AGTTTAAGTC TTGCTTGCGC TCCCTTGGCT ATGACCTGCC CATGGTTGAG
    GAAGGAGAGC CTGACCCCGA ATTTGAGTCT ATTCTTGACA CTGTTGATCC CAACAGGGAT
    GGCCACGTCT CGCTGCAGGA GTACATGGCG TTCATGATCA GCAGGGAAAC AGAGAACGTG
    AAATCCAGCG AGGAGATTGA GAGTGCTTTC CGTGCCCTCA GCTCGGAGGG GAAGCCTTAC
    GTGACCAAGG AGGAGCTCTA CCAGAACCTG ACCCGGGAGC AGGCCGACTA CTGCATTTCT
    CACATGAAGC CCTACATGGA TGGCAAGGGC AGAGAACTTC CTTCTGCCTA CGACTACATA
    GAATTTACAC GTTCACTCTT TGTGAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa43320
    Clone ID Related Accession (Same CDS sequence) XM_015279294.1 , XM_015279294.2
    Accession Version XM_015279294.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7395bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
    TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG
    TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC
    TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT
    GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT
    GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG
    ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAA CTCGTCTACA GGAGCTGCAC
    CGACTATGGG AGTTACTGCT GGAAAAAATG AGAGAGAAGG GAGTGAAGCT GTTGCAAGCA
    CAGAAGCTGG TGCAATTCTT ACGGGAATGT GAAGATGTCA TGGACTGGAT CAATGACAAG
    GAAGCAATAG TGACCTCAGA AGAGCTTGGA CAGGACTTAG AGCATGTTGA GGTTTTGCAG
    AAGAAATTTG AAGAGTTCCA AACAGATCTG GCAGCTCATG AGGAGAGAGT GAATGAAGTG
    AACCAGTTCG CTGGCAAACT TATCCAGGAA CAGCACCCTG AGGAGGAACT TATAAAGTCC
    AAACAGGATG AAGTAAATGC AAGCTGGCAG CGTCTTAAGG GGCTTGCCCA ACAGAGGCAA
    GGAAAACTCT TTGGGGCAGC TGAAGTTCAG CGCTTCAACA GGGATGTGGA TGAAACTATC
    AGCTGGATTA AGGAGAAAGG GCAGTTGATG GCCTCAGATG ACTTTGGCAG AGACTTGGCC
    AGCGTGCAAG CTTTACTACG TAAGCATGAA GGCCTGGAAA GAGATCTTGC AGCTTTGGAG
    GATAAGGTTA AGGCCCTCTG TGCAGAAGCT GACCGTTTGC AGCAGTCTCA CCCTATAAAT
    GCTTCTCAAA TTCAAGTGAA ACGGGAGGAA CTGATTGCCA ACTGGGAGCA GATCCGAACT
    CTGGCAGCAG AGAGGCATGC TCGCCTTAAT GACTCCTACA GGTTGCAGCG CTTTCTTGCA
    GATTTCCGGG ACCTCACTAG CTGGGTAACT GAGATGAAGG CTCTGATAAA TGCTGATGAG
    CTTGCCAATG ATGTGGCTGG AGCAGAAGCC CTTCTAGATA GACATCAGGA ACATAAGGGA
    GAAATTGATG CTCATGAGGA TAGCTTCAGA TCTGCTGATG AGTCTGGGCA GGCTTTGCTT
    GCAGCTGGGC ACTATGCTTC TGATGAAGTT AAAGAAAAGC TGACTATCCT CTCAGATGAA
    AGATCAGCCT TGCTGGAACT ATGGGAGCTT CGCAGGCAAC AGTATGAGCA GTGCATGGAT
    CTGCAGCTTT TCTACAGAGA TACTGAACAA GTTGACAACT GGATGAGCAA ACAAGAAGCT
    TTTCTGCTGA ACGAAGACCT TGGTGATTCT CTGGATAGCG TGGAGGCTCT TCTAAAGAAG
    CATGAAGATT TTGAGAAATC CCTAAGTGCT CAAGAGGAGA AGATCACAGC ACTGGATGAG
    TTTGCTACTA AACTGATTCA AAATAACCAT TATGCCATGG ATGACGTTGC TACGCGCAGA
    GATGCTCTTC TGAGTCGCCG AAATGCTCTT CATGAAAGAG CCATGAAGCG CCGTGCCCAA
    CTGGCAGACT CTTTCCATCT CCAGCAGTTT TTCAGGGACT CTGATGAACT GAAGAGTTGG
    GTTAATGAAA AAATGAAAAC TGCTACCGAT GAGGCTTACA AGGATCCATC CAACTTGCAA
    GGTAAAGTTC AGAAGCATCA GGCTTTTGAA GCAGAGCTTT CTGCTAATCA GAGTCGTATT
    GATGCACTGG AGAAAGCTGG CCAGAAGTTG ATTGATGTCA ATCACTATGC ATCTGATGAG
    GTGGCAGCTC GCATGAATGA AGTCATCAGC TTGTGGAAGA AACTTCTGGA AGCCACCGAG
    CTCAAAGGTA TAAAGCTGCG TGAAGCCAAT CAACAGCAGC AATTTAATCG CAATGTAGAA
    GACATTGAGT TGTGGCTGTA TGAAGTGGAG GGTCACTTGG CTTCTGATGA TTATGGAAAA
    GATCTTACCA GCGTTCAGAA TCTTCAGAAG AAACATGCCC TGCTAGAGGC AGATGTTGCT
    GCCCATCAGG ATCGGATAGA TGGCATTACC ATCCAGGCAC GCCAGTTCCA AGATGCTGGG
    CACTTTGATG CTGACAATAT CAAGAAGAAA CAAGAAGCTT TAGTAGCTCG TTATGAAGCT
    CTGAAGGATC CTATGGTGGC TCGCAAGCAG AAACTTGCAG ATTCTCTTCG CCTGCAGCAG
    CTTTTCCGTG ACATCGAGGA TGAAGAGACC TGGATCAGGG AAAAGGAACC TATTGCAGCC
    TCAACAAACC GAGGCAAGGA CTTAATTGGT GTCCAGAATC TGCTAAAGAA GCACCAGGCT
    TTGCAGGCAG AAATTGCAGG CCATGAGCCT CGCATTAAAG CAGTCACACA AAAAGGAAAT
    GCGATGGTGG AAGAGGGACA TTTTGCTGCT GAAGATGTGA AGATCAAATT GAATGAACTA
    AACCAAAAAT GGGACTCTCT GAAAGCCAAG GCATCTCAAC GGCGACAGGA TCTAGAGGAT
    TCTCTGCAAG CTCAGCAGTA TTTTGCTGAT GCTAATGAGG CAGAATCATG GATGAGGGAA
    AAGGAGCCCA TTGTAGGCAG CACGGATTAT GGAAAGGATG AAGACTCTGC TGAGGCTCTC
    CTGAAGAAGC ATGAAGCTTT GATGTCTGAT CTTTCTGCTT ACGGCAGTAG CATACAGGCA
    TTAAGGGAAC AGGCCCAGTC CTGCAGGCAA CAAGTTGCTC CCACTGATGA TGAAACTGGA
    AAAGAGCTTG TGCTAGCACT CTATGATTAC CAAGAGAAGA GTCCTCGGGA GGTGACTATG
    AAGAAAGGAG ATATTCTAAC CCTGCTCAAC AGCACCAACA AGGACTGGTG GAAGGTTGAA
    GTTAACGATC GTCAGGGCTT TGTACCAGCT GCCTATGTGA AAAAACTAGA TCCTGCCCAG
    TCTGCATCCC GAGAGAATCT TCTAGAAGAG CAAGGCAGCA TAGCATTGCG ACAGGAGCAA
    ATTGACAACC AGTATCATTC CCTTCTTGAG CTGGGAGAAA AACGTAAAGG CATGCTAGAA
    AAAAGCTGCA AGAAGTTTAT GCTTTTCCGT GAGGCTAATG AGCTTCAACA GTGGATCAAT
    GAGAAGGAAG CTGCGCTCAC TAATGAGGAA GTGGGTGCTG ATTTGGAGCA GGTGGAGGTG
    CTACAGAAGA AGTTTGATGA TTTTCAGAAG GATTTAAAAG CTAATGAGTC ACGACTGAAG
    GACATAAACA AGGTTGCAAA TGACCTGGAG TCTGAAGGGC TGATGGCAGA GGAAGTGCAA
    GCAGTAGAGC ACCAGGAAGT CTATGGAATG ATGCCCAGAG ATGAAACTGA TTCTAAGACA
    GCCTCTCCTT GGAAGTCTGC ACGTATGATG GTACACACAG TGGCAACGTT TAACTCAATC
    AAGGAACTGA ATGAACGCTG GAGATCCCTG CAGCAGTTGG CAGAGGAGCG AAGCCAGCTG
    TTGGGCAGCG CTCATGAAGT CCAGAGGTTC CACAGAGATG CTGATGAAAC AAAAGAATGG
    ATAGAAGAGA AGAATCAAGC ATTAAATACC GACAACTATG GACATGACTT GGCCAGTGTG
    CAGGCTCTGC AGCGCAAGCA TGAAGGCTTT GAGAGAGACT TGGCAGCTCT TGGAGACAAG
    GTGAATTCTC TTGGTGAAAC TGCCCAGCGT CTGATCCAGT CACATCCAGA ATCAGCTGAA
    GATCTTCAAG AAAAATGTAC TGAGTTGAAC CAAGCCTGGA GTAGTCTGGG AAAACGTGCT
    GACCAACGCA AAGAGAAGCT TGGAGATTCT CATGACCTGC AGCGTTTCCT CAGTGATTTT
    AGAGACCTTA TGTCTTGGAT CAATGGAATC CGGGGTCTGG TCTCCTCAGA TGAACTTGCA
    AAAGATGTGA CTGGAGCTGA AGCTCTGTTG GAAAGGCATC AGGAGCACCG TACTGAAATA
    GATGCACGGG CTGGCACTTT TCAGGCATTT GAACAGTTTG GACAGCAGCT TCTGGCTCGT
    GGACACTATG CCAGCCCAGA GATTAAGGAG AAACTGGATA TTCTAGATCA AGAACGGACA
    GACCTAGAGA AGGCCTGGGT CCAGCGCAGA ATGATGCTAG ACCAGTGCTT AGAACTACAG
    CTGTTTCATC GGGATTGTGA ACAAGCTGAA AACTGGATGG CTGCCCGAGA GGCGTTCCTA
    AATACAGAAG ACAAAGGAGA CTCCTTAGAC AGCGTGGAGG CACTCATCAA GAAGCATGAA
    GATTTCGATA AAGCAATCAA TGTCCAGGAA GAGAAAATTG CTGTCTTGCA GTCTTTTGCT
    GACCAACTGA TTGCTGCTGA TCATTATGCA AAAGGAGTCA TTGCTAACAG ACGCAATGAG
    GTTCTGGACA GGTGGCGTCG TCTGAAGGCT CAGATGATTG AGAAGAGATC TAAGCTGGGA
    GAATCTCAGA CCCTCCAACA GTTCAGTCGT GATGTTGATG AAATAGAAGC TTGGATCAGT
    GAAAAGCTTC AAACTGCAAG TGATGAGTCA TATAAGGATC CCACAAACAT CCAGAGCAAG
    CACCAGAAGC ACCAAGCCTT TGAAGCTGAG CTCCACGCCA ACGCAGATCG GATTCGTGGA
    GTCATTGAAA TGGGGAACTC TCTTATTGAA AGAGGAGCGT GTGCTGGCAG CGAGGATGCT
    GTGAAGGCTC GGCTGGCTGC CCTGGCTGAC CAATGGGAGT TCCTGGTCCA GAAGTCATCA
    GAGAAGAGTC AGAAACTGAA AGAAGCAAAT AAACAGCAGA ATTTCAATAC CGGCATCAAG
    GACTTTGATT TCTGGCTTTC AGAGGTGGAA GCTTTGTTGG CATCTGAAGA CTATGGGAAG
    GACTTGGCAT CAGTGAACAA CCTTCTGAAG AAGCACCAAT TACTGGAAGC TGATATATCT
    GCTCATGAGG ATCGGCTGAA GGACCTGAAC AGCCAGGCTG ACAGTTTGAT GACCAGCAGT
    GCTTTCGATA CCTCCCAAGT AAAGGATAAA CGTGAAACTA TAAATGGGCG CTTCCAGAGA
    ATCAAGAGCA TGGCAGCTGC CCGCCGTGCG AAGCTCAACG AGTCGCACCG CTTGCATCAG
    TTCTTCCGTG ACATGGATGA TGAGGAGTCC TGGATCAAAG AGAAGAAACT GTTGGTGAGC
    TCAGAGGACT ATGGCAGAGA CCTGACTGGT GTGCAGAACC TGAGGAAGAA ACATAAGCGC
    TTGGAAGCAG AATTAGCTGC CCATGAACCT GCTATCCAGG GTGTTCTAGA CACTGGCAAG
    AAGCTTTCAG ATGATAACAC AATTGGGAAG GAGGAGATAC AGCAAAGACT GGCTCAGTTT
    GTGGACCACT GGAAAGAGTT AAAACAGCTG GCAGCTGCTC GGGGGCAGCG TCTGGAGGAG
    TCCCTGGAGT ACCAACAGTT TGTAGCAAAT GTTGAGGAAG AGGAGGCATG GATCAACGAG
    AAAATGACAT TGGTAGCCAG TGAGGATTAT GGAGACACAC TTGCTGCTAT CCAGGGCTTG
    CTGAAGAAGC ATGAAGCATT CGAGACTGAC TTTACTGTCC ACAAAGACAG AGTGAATGAT
    GTTTGCGCTA ATGGAGAGGA TCTCATTAAA AAGAATAATC ACCATGTGGA GAACATTACT
    GCTAAGATGA AGGGCCTCAA GGGCAAGGTA TCAGATCTGG AGAAAGCCGC AGCTCAGAGG
    AAAGCCAAAC TGGATGAGAA CTCTGCCTTC CTCCAGTTCA ACTGGAAAGC AGATGTGGTG
    GAGTCATGGA TAGGTGAGAA GGAAAACAGT CTGAAGACAG ATGATTATGG CCGTGACCTC
    TCCTCTGTGC AAACACTACT CACCAAACAG GAAACCTTTG ATGCTGGACT TCAGGCTTTC
    CAGCAGGAGG GAATTGCAAA CATCACTGCT CTGAAAGACC AGCTACTGGC AGCCAAACAT
    ATCCAATCAA AGGCCATTGA GGTTCGTCAC GCTTCCTTGA TGAAACGCTG GAATCAGCTG
    CTTGCTAATT CTGCAGCCAG GAAAAAGAAA CTCTTGGAGG CTCAGGAGCA CTTCAGAAAG
    GTTGAAGATC TCTTCTTGAC TTTTGCCAAG AAGGCCTCTG CCTTCAACAG TTGGTTTGAG
    AATGCTGAGG AGGACCTGAC GGATCCCGTG CGCTGCAATT CCCTGGAAGA AATCAAAGCA
    CTGCGAGAAG CCCACGATGC GTTCCGTTCC TCACTTAGCT CTGCCCAAGC TGACTTCAAC
    CAGCTGGCAG AGCTTGATCG GCAGATCAAG AGCTTCCGTG TAGCCTCCAA CCCGTACACT
    TGGTTTACTA TGGAGGCTCT TGAAGAAACT TGGAGGAATC TGCAGAAAAT TATCAAGGAG
    CGTGAATTGG AGTTGCAGAA GGAGCAGCGG AGGCAGGAAG AAAATGACAA GTTGCGCCAG
    GAGTTTGCTC AGCATGCTAA TGCCTTCCAC CAGTGGATTC AGGAGACAAG GACTTACCTG
    CTAGATGGGT CCTGCATGGT GGAGGAGTCG GGAACACTGG AATCCCAGCT GGAAGCTACT
    AAACGCAAGC ACCAGGAGAT CCGGGCTATG AGGAGCCAGC TGAAGAAGAT TGAGGACCTT
    GGAGCAGCCA TGGAAGAGGC ACTTATCTTG GACAACAAAT ACACAGAGCA CAGCACCGTG
    GGGCTGGCGC AGCAGTGGGA CCAGCTTGAC CAGCTGGGAA TGAGAATGCA GCACAATCTG
    GAACAGCAGA TTCAAGCTCG AAACACAACT GGAGTCACAG AGGAGGCCCT GAAGGAGTTC
    AGCATGATGT TCAAGCACTT TGACAAGGAC AAATCTGGAC GTCTTAATCA CCAGGAGTTT
    AAGTCTTGCT TGCGCTCCCT TGGCTATGAC CTGCCCATGG TTGAGGAAGG AGAGCCTGAC
    CCCGAATTTG AGTCTATTCT TGACACTGTT GATCCCAACA GGGATGGCCA CGTCTCGCTG
    CAGGAGTACA TGGCGTTCAT GATCAGCAGG GAAACAGAGA ACGTGAAATC CAGCGAGGAG
    ATTGAGAGTG CTTTCCGTGC CCTCAGCTCG GAGGGGAAGC CTTACGTGAC CAAGGAGGAG
    CTCTACCAGA ACCTGACCCG GGAGCAGGCC GACTACTGCA TTTCTCACAT GAAGCCCTAC
    ATGGATGGCA AGGGCAGAGA ACTTCCTTCT GCCTACGACT ACATAGAATT TACACGTTCA
    CTCTTTGTGA ATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015134780.1
    CDS9..7403
    Translation

    Target ORF information:

    RefSeq Version XM_015279294.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015279294.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
    TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG
    TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC
    TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT
    GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT
    GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG
    ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAA CTCGTCTACA GGAGCTGCAC
    CGACTATGGG AGTTACTGCT GGAAAAAATG AGAGAGAAGG GAGTGAAGCT GTTGCAAGCA
    CAGAAGCTGG TGCAATTCTT ACGGGAATGT GAAGATGTCA TGGACTGGAT CAATGACAAG
    GAAGCAATAG TGACCTCAGA AGAGCTTGGA CAGGACTTAG AGCATGTTGA GGTTTTGCAG
    AAGAAATTTG AAGAGTTCCA AACAGATCTG GCAGCTCATG AGGAGAGAGT GAATGAAGTG
    AACCAGTTCG CTGGCAAACT TATCCAGGAA CAGCACCCTG AGGAGGAACT TATAAAGTCC
    AAACAGGATG AAGTAAATGC AAGCTGGCAG CGTCTTAAGG GGCTTGCCCA ACAGAGGCAA
    GGAAAACTCT TTGGGGCAGC TGAAGTTCAG CGCTTCAACA GGGATGTGGA TGAAACTATC
    AGCTGGATTA AGGAGAAAGG GCAGTTGATG GCCTCAGATG ACTTTGGCAG AGACTTGGCC
    AGCGTGCAAG CTTTACTACG TAAGCATGAA GGCCTGGAAA GAGATCTTGC AGCTTTGGAG
    GATAAGGTTA AGGCCCTCTG TGCAGAAGCT GACCGTTTGC AGCAGTCTCA CCCTATAAAT
    GCTTCTCAAA TTCAAGTGAA ACGGGAGGAA CTGATTGCCA ACTGGGAGCA GATCCGAACT
    CTGGCAGCAG AGAGGCATGC TCGCCTTAAT GACTCCTACA GGTTGCAGCG CTTTCTTGCA
    GATTTCCGGG ACCTCACTAG CTGGGTAACT GAGATGAAGG CTCTGATAAA TGCTGATGAG
    CTTGCCAATG ATGTGGCTGG AGCAGAAGCC CTTCTAGATA GACATCAGGA ACATAAGGGA
    GAAATTGATG CTCATGAGGA TAGCTTCAGA TCTGCTGATG AGTCTGGGCA GGCTTTGCTT
    GCAGCTGGGC ACTATGCTTC TGATGAAGTT AAAGAAAAGC TGACTATCCT CTCAGATGAA
    AGATCAGCCT TGCTGGAACT ATGGGAGCTT CGCAGGCAAC AGTATGAGCA GTGCATGGAT
    CTGCAGCTTT TCTACAGAGA TACTGAACAA GTTGACAACT GGATGAGCAA ACAAGAAGCT
    TTTCTGCTGA ACGAAGACCT TGGTGATTCT CTGGATAGCG TGGAGGCTCT TCTAAAGAAG
    CATGAAGATT TTGAGAAATC CCTAAGTGCT CAAGAGGAGA AGATCACAGC ACTGGATGAG
    TTTGCTACTA AACTGATTCA AAATAACCAT TATGCCATGG ATGACGTTGC TACGCGCAGA
    GATGCTCTTC TGAGTCGCCG AAATGCTCTT CATGAAAGAG CCATGAAGCG CCGTGCCCAA
    CTGGCAGACT CTTTCCATCT CCAGCAGTTT TTCAGGGACT CTGATGAACT GAAGAGTTGG
    GTTAATGAAA AAATGAAAAC TGCTACCGAT GAGGCTTACA AGGATCCATC CAACTTGCAA
    GGTAAAGTTC AGAAGCATCA GGCTTTTGAA GCAGAGCTTT CTGCTAATCA GAGTCGTATT
    GATGCACTGG AGAAAGCTGG CCAGAAGTTG ATTGATGTCA ATCACTATGC ATCTGATGAG
    GTGGCAGCTC GCATGAATGA AGTCATCAGC TTGTGGAAGA AACTTCTGGA AGCCACCGAG
    CTCAAAGGTA TAAAGCTGCG TGAAGCCAAT CAACAGCAGC AATTTAATCG CAATGTAGAA
    GACATTGAGT TGTGGCTGTA TGAAGTGGAG GGTCACTTGG CTTCTGATGA TTATGGAAAA
    GATCTTACCA GCGTTCAGAA TCTTCAGAAG AAACATGCCC TGCTAGAGGC AGATGTTGCT
    GCCCATCAGG ATCGGATAGA TGGCATTACC ATCCAGGCAC GCCAGTTCCA AGATGCTGGG
    CACTTTGATG CTGACAATAT CAAGAAGAAA CAAGAAGCTT TAGTAGCTCG TTATGAAGCT
    CTGAAGGATC CTATGGTGGC TCGCAAGCAG AAACTTGCAG ATTCTCTTCG CCTGCAGCAG
    CTTTTCCGTG ACATCGAGGA TGAAGAGACC TGGATCAGGG AAAAGGAACC TATTGCAGCC
    TCAACAAACC GAGGCAAGGA CTTAATTGGT GTCCAGAATC TGCTAAAGAA GCACCAGGCT
    TTGCAGGCAG AAATTGCAGG CCATGAGCCT CGCATTAAAG CAGTCACACA AAAAGGAAAT
    GCGATGGTGG AAGAGGGACA TTTTGCTGCT GAAGATGTGA AGATCAAATT GAATGAACTA
    AACCAAAAAT GGGACTCTCT GAAAGCCAAG GCATCTCAAC GGCGACAGGA TCTAGAGGAT
    TCTCTGCAAG CTCAGCAGTA TTTTGCTGAT GCTAATGAGG CAGAATCATG GATGAGGGAA
    AAGGAGCCCA TTGTAGGCAG CACGGATTAT GGAAAGGATG AAGACTCTGC TGAGGCTCTC
    CTGAAGAAGC ATGAAGCTTT GATGTCTGAT CTTTCTGCTT ACGGCAGTAG CATACAGGCA
    TTAAGGGAAC AGGCCCAGTC CTGCAGGCAA CAAGTTGCTC CCACTGATGA TGAAACTGGA
    AAAGAGCTTG TGCTAGCACT CTATGATTAC CAAGAGAAGA GTCCTCGGGA GGTGACTATG
    AAGAAAGGAG ATATTCTAAC CCTGCTCAAC AGCACCAACA AGGACTGGTG GAAGGTTGAA
    GTTAACGATC GTCAGGGCTT TGTACCAGCT GCCTATGTGA AAAAACTAGA TCCTGCCCAG
    TCTGCATCCC GAGAGAATCT TCTAGAAGAG CAAGGCAGCA TAGCATTGCG ACAGGAGCAA
    ATTGACAACC AGTATCATTC CCTTCTTGAG CTGGGAGAAA AACGTAAAGG CATGCTAGAA
    AAAAGCTGCA AGAAGTTTAT GCTTTTCCGT GAGGCTAATG AGCTTCAACA GTGGATCAAT
    GAGAAGGAAG CTGCGCTCAC TAATGAGGAA GTGGGTGCTG ATTTGGAGCA GGTGGAGGTG
    CTACAGAAGA AGTTTGATGA TTTTCAGAAG GATTTAAAAG CTAATGAGTC ACGACTGAAG
    GACATAAACA AGGTTGCAAA TGACCTGGAG TCTGAAGGGC TGATGGCAGA GGAAGTGCAA
    GCAGTAGAGC ACCAGGAAGT CTATGGAATG ATGCCCAGAG ATGAAACTGA TTCTAAGACA
    GCCTCTCCTT GGAAGTCTGC ACGTATGATG GTACACACAG TGGCAACGTT TAACTCAATC
    AAGGAACTGA ATGAACGCTG GAGATCCCTG CAGCAGTTGG CAGAGGAGCG AAGCCAGCTG
    TTGGGCAGCG CTCATGAAGT CCAGAGGTTC CACAGAGATG CTGATGAAAC AAAAGAATGG
    ATAGAAGAGA AGAATCAAGC ATTAAATACC GACAACTATG GACATGACTT GGCCAGTGTG
    CAGGCTCTGC AGCGCAAGCA TGAAGGCTTT GAGAGAGACT TGGCAGCTCT TGGAGACAAG
    GTGAATTCTC TTGGTGAAAC TGCCCAGCGT CTGATCCAGT CACATCCAGA ATCAGCTGAA
    GATCTTCAAG AAAAATGTAC TGAGTTGAAC CAAGCCTGGA GTAGTCTGGG AAAACGTGCT
    GACCAACGCA AAGAGAAGCT TGGAGATTCT CATGACCTGC AGCGTTTCCT CAGTGATTTT
    AGAGACCTTA TGTCTTGGAT CAATGGAATC CGGGGTCTGG TCTCCTCAGA TGAACTTGCA
    AAAGATGTGA CTGGAGCTGA AGCTCTGTTG GAAAGGCATC AGGAGCACCG TACTGAAATA
    GATGCACGGG CTGGCACTTT TCAGGCATTT GAACAGTTTG GACAGCAGCT TCTGGCTCGT
    GGACACTATG CCAGCCCAGA GATTAAGGAG AAACTGGATA TTCTAGATCA AGAACGGACA
    GACCTAGAGA AGGCCTGGGT CCAGCGCAGA ATGATGCTAG ACCAGTGCTT AGAACTACAG
    CTGTTTCATC GGGATTGTGA ACAAGCTGAA AACTGGATGG CTGCCCGAGA GGCGTTCCTA
    AATACAGAAG ACAAAGGAGA CTCCTTAGAC AGCGTGGAGG CACTCATCAA GAAGCATGAA
    GATTTCGATA AAGCAATCAA TGTCCAGGAA GAGAAAATTG CTGTCTTGCA GTCTTTTGCT
    GACCAACTGA TTGCTGCTGA TCATTATGCA AAAGGAGTCA TTGCTAACAG ACGCAATGAG
    GTTCTGGACA GGTGGCGTCG TCTGAAGGCT CAGATGATTG AGAAGAGATC TAAGCTGGGA
    GAATCTCAGA CCCTCCAACA GTTCAGTCGT GATGTTGATG AAATAGAAGC TTGGATCAGT
    GAAAAGCTTC AAACTGCAAG TGATGAGTCA TATAAGGATC CCACAAACAT CCAGAGCAAG
    CACCAGAAGC ACCAAGCCTT TGAAGCTGAG CTCCACGCCA ACGCAGATCG GATTCGTGGA
    GTCATTGAAA TGGGGAACTC TCTTATTGAA AGAGGAGCGT GTGCTGGCAG CGAGGATGCT
    GTGAAGGCTC GGCTGGCTGC CCTGGCTGAC CAATGGGAGT TCCTGGTCCA GAAGTCATCA
    GAGAAGAGTC AGAAACTGAA AGAAGCAAAT AAACAGCAGA ATTTCAATAC CGGCATCAAG
    GACTTTGATT TCTGGCTTTC AGAGGTGGAA GCTTTGTTGG CATCTGAAGA CTATGGGAAG
    GACTTGGCAT CAGTGAACAA CCTTCTGAAG AAGCACCAAT TACTGGAAGC TGATATATCT
    GCTCATGAGG ATCGGCTGAA GGACCTGAAC AGCCAGGCTG ACAGTTTGAT GACCAGCAGT
    GCTTTCGATA CCTCCCAAGT AAAGGATAAA CGTGAAACTA TAAATGGGCG CTTCCAGAGA
    ATCAAGAGCA TGGCAGCTGC CCGCCGTGCG AAGCTCAACG AGTCGCACCG CTTGCATCAG
    TTCTTCCGTG ACATGGATGA TGAGGAGTCC TGGATCAAAG AGAAGAAACT GTTGGTGAGC
    TCAGAGGACT ATGGCAGAGA CCTGACTGGT GTGCAGAACC TGAGGAAGAA ACATAAGCGC
    TTGGAAGCAG AATTAGCTGC CCATGAACCT GCTATCCAGG GTGTTCTAGA CACTGGCAAG
    AAGCTTTCAG ATGATAACAC AATTGGGAAG GAGGAGATAC AGCAAAGACT GGCTCAGTTT
    GTGGACCACT GGAAAGAGTT AAAACAGCTG GCAGCTGCTC GGGGGCAGCG TCTGGAGGAG
    TCCCTGGAGT ACCAACAGTT TGTAGCAAAT GTTGAGGAAG AGGAGGCATG GATCAACGAG
    AAAATGACAT TGGTAGCCAG TGAGGATTAT GGAGACACAC TTGCTGCTAT CCAGGGCTTG
    CTGAAGAAGC ATGAAGCATT CGAGACTGAC TTTACTGTCC ACAAAGACAG AGTGAATGAT
    GTTTGCGCTA ATGGAGAGGA TCTCATTAAA AAGAATAATC ACCATGTGGA GAACATTACT
    GCTAAGATGA AGGGCCTCAA GGGCAAGGTA TCAGATCTGG AGAAAGCCGC AGCTCAGAGG
    AAAGCCAAAC TGGATGAGAA CTCTGCCTTC CTCCAGTTCA ACTGGAAAGC AGATGTGGTG
    GAGTCATGGA TAGGTGAGAA GGAAAACAGT CTGAAGACAG ATGATTATGG CCGTGACCTC
    TCCTCTGTGC AAACACTACT CACCAAACAG GAAACCTTTG ATGCTGGACT TCAGGCTTTC
    CAGCAGGAGG GAATTGCAAA CATCACTGCT CTGAAAGACC AGCTACTGGC AGCCAAACAT
    ATCCAATCAA AGGCCATTGA GGTTCGTCAC GCTTCCTTGA TGAAACGCTG GAATCAGCTG
    CTTGCTAATT CTGCAGCCAG GAAAAAGAAA CTCTTGGAGG CTCAGGAGCA CTTCAGAAAG
    GTTGAAGATC TCTTCTTGAC TTTTGCCAAG AAGGCCTCTG CCTTCAACAG TTGGTTTGAG
    AATGCTGAGG AGGACCTGAC GGATCCCGTG CGCTGCAATT CCCTGGAAGA AATCAAAGCA
    CTGCGAGAAG CCCACGATGC GTTCCGTTCC TCACTTAGCT CTGCCCAAGC TGACTTCAAC
    CAGCTGGCAG AGCTTGATCG GCAGATCAAG AGCTTCCGTG TAGCCTCCAA CCCGTACACT
    TGGTTTACTA TGGAGGCTCT TGAAGAAACT TGGAGGAATC TGCAGAAAAT TATCAAGGAG
    CGTGAATTGG AGTTGCAGAA GGAGCAGCGG AGGCAGGAAG AAAATGACAA GTTGCGCCAG
    GAGTTTGCTC AGCATGCTAA TGCCTTCCAC CAGTGGATTC AGGAGACAAG GACTTACCTG
    CTAGATGGGT CCTGCATGGT GGAGGAGTCG GGAACACTGG AATCCCAGCT GGAAGCTACT
    AAACGCAAGC ACCAGGAGAT CCGGGCTATG AGGAGCCAGC TGAAGAAGAT TGAGGACCTT
    GGAGCAGCCA TGGAAGAGGC ACTTATCTTG GACAACAAAT ACACAGAGCA CAGCACCGTG
    GGGCTGGCGC AGCAGTGGGA CCAGCTTGAC CAGCTGGGAA TGAGAATGCA GCACAATCTG
    GAACAGCAGA TTCAAGCTCG AAACACAACT GGAGTCACAG AGGAGGCCCT GAAGGAGTTC
    AGCATGATGT TCAAGCACTT TGACAAGGAC AAATCTGGAC GTCTTAATCA CCAGGAGTTT
    AAGTCTTGCT TGCGCTCCCT TGGCTATGAC CTGCCCATGG TTGAGGAAGG AGAGCCTGAC
    CCCGAATTTG AGTCTATTCT TGACACTGTT GATCCCAACA GGGATGGCCA CGTCTCGCTG
    CAGGAGTACA TGGCGTTCAT GATCAGCAGG GAAACAGAGA ACGTGAAATC CAGCGAGGAG
    ATTGAGAGTG CTTTCCGTGC CCTCAGCTCG GAGGGGAAGC CTTACGTGAC CAAGGAGGAG
    CTCTACCAGA ACCTGACCCG GGAGCAGGCC GACTACTGCA TTTCTCACAT GAAGCCCTAC
    ATGGATGGCA AGGGCAGAGA ACTTCCTTCT GCCTACGACT ACATAGAATT TACACGTTCA
    CTCTTTGTGA ATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa43320
    Clone ID Related Accession (Same CDS sequence) XM_015279294.1 , XM_015279294.2
    Accession Version XM_015279294.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7395bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015279294.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
    TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG
    TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC
    TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT
    GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT
    GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG
    ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAA CTCGTCTACA GGAGCTGCAC
    CGACTATGGG AGTTACTGCT GGAAAAAATG AGAGAGAAGG GAGTGAAGCT GTTGCAAGCA
    CAGAAGCTGG TGCAATTCTT ACGGGAATGT GAAGATGTCA TGGACTGGAT CAATGACAAG
    GAAGCAATAG TGACCTCAGA AGAGCTTGGA CAGGACTTAG AGCATGTTGA GGTTTTGCAG
    AAGAAATTTG AAGAGTTCCA AACAGATCTG GCAGCTCATG AGGAGAGAGT GAATGAAGTG
    AACCAGTTCG CTGGCAAACT TATCCAGGAA CAGCACCCTG AGGAGGAACT TATAAAGTCC
    AAACAGGATG AAGTAAATGC AAGCTGGCAG CGTCTTAAGG GGCTTGCCCA ACAGAGGCAA
    GGAAAACTCT TTGGGGCAGC TGAAGTTCAG CGCTTCAACA GGGATGTGGA TGAAACTATC
    AGCTGGATTA AGGAGAAAGG GCAGTTGATG GCCTCAGATG ACTTTGGCAG AGACTTGGCC
    AGCGTGCAAG CTTTACTACG TAAGCATGAA GGCCTGGAAA GAGATCTTGC AGCTTTGGAG
    GATAAGGTTA AGGCCCTCTG TGCAGAAGCT GACCGTTTGC AGCAGTCTCA CCCTATAAAT
    GCTTCTCAAA TTCAAGTGAA ACGGGAGGAA CTGATTGCCA ACTGGGAGCA GATCCGAACT
    CTGGCAGCAG AGAGGCATGC TCGCCTTAAT GACTCCTACA GGTTGCAGCG CTTTCTTGCA
    GATTTCCGGG ACCTCACTAG CTGGGTAACT GAGATGAAGG CTCTGATAAA TGCTGATGAG
    CTTGCCAATG ATGTGGCTGG AGCAGAAGCC CTTCTAGATA GACATCAGGA ACATAAGGGA
    GAAATTGATG CTCATGAGGA TAGCTTCAGA TCTGCTGATG AGTCTGGGCA GGCTTTGCTT
    GCAGCTGGGC ACTATGCTTC TGATGAAGTT AAAGAAAAGC TGACTATCCT CTCAGATGAA
    AGATCAGCCT TGCTGGAACT ATGGGAGCTT CGCAGGCAAC AGTATGAGCA GTGCATGGAT
    CTGCAGCTTT TCTACAGAGA TACTGAACAA GTTGACAACT GGATGAGCAA ACAAGAAGCT
    TTTCTGCTGA ACGAAGACCT TGGTGATTCT CTGGATAGCG TGGAGGCTCT TCTAAAGAAG
    CATGAAGATT TTGAGAAATC CCTAAGTGCT CAAGAGGAGA AGATCACAGC ACTGGATGAG
    TTTGCTACTA AACTGATTCA AAATAACCAT TATGCCATGG ATGACGTTGC TACGCGCAGA
    GATGCTCTTC TGAGTCGCCG AAATGCTCTT CATGAAAGAG CCATGAAGCG CCGTGCCCAA
    CTGGCAGACT CTTTCCATCT CCAGCAGTTT TTCAGGGACT CTGATGAACT GAAGAGTTGG
    GTTAATGAAA AAATGAAAAC TGCTACCGAT GAGGCTTACA AGGATCCATC CAACTTGCAA
    GGTAAAGTTC AGAAGCATCA GGCTTTTGAA GCAGAGCTTT CTGCTAATCA GAGTCGTATT
    GATGCACTGG AGAAAGCTGG CCAGAAGTTG ATTGATGTCA ATCACTATGC ATCTGATGAG
    GTGGCAGCTC GCATGAATGA AGTCATCAGC TTGTGGAAGA AACTTCTGGA AGCCACCGAG
    CTCAAAGGTA TAAAGCTGCG TGAAGCCAAT CAACAGCAGC AATTTAATCG CAATGTAGAA
    GACATTGAGT TGTGGCTGTA TGAAGTGGAG GGTCACTTGG CTTCTGATGA TTATGGAAAA
    GATCTTACCA GCGTTCAGAA TCTTCAGAAG AAACATGCCC TGCTAGAGGC AGATGTTGCT
    GCCCATCAGG ATCGGATAGA TGGCATTACC ATCCAGGCAC GCCAGTTCCA AGATGCTGGG
    CACTTTGATG CTGACAATAT CAAGAAGAAA CAAGAAGCTT TAGTAGCTCG TTATGAAGCT
    CTGAAGGATC CTATGGTGGC TCGCAAGCAG AAACTTGCAG ATTCTCTTCG CCTGCAGCAG
    CTTTTCCGTG ACATCGAGGA TGAAGAGACC TGGATCAGGG AAAAGGAACC TATTGCAGCC
    TCAACAAACC GAGGCAAGGA CTTAATTGGT GTCCAGAATC TGCTAAAGAA GCACCAGGCT
    TTGCAGGCAG AAATTGCAGG CCATGAGCCT CGCATTAAAG CAGTCACACA AAAAGGAAAT
    GCGATGGTGG AAGAGGGACA TTTTGCTGCT GAAGATGTGA AGATCAAATT GAATGAACTA
    AACCAAAAAT GGGACTCTCT GAAAGCCAAG GCATCTCAAC GGCGACAGGA TCTAGAGGAT
    TCTCTGCAAG CTCAGCAGTA TTTTGCTGAT GCTAATGAGG CAGAATCATG GATGAGGGAA
    AAGGAGCCCA TTGTAGGCAG CACGGATTAT GGAAAGGATG AAGACTCTGC TGAGGCTCTC
    CTGAAGAAGC ATGAAGCTTT GATGTCTGAT CTTTCTGCTT ACGGCAGTAG CATACAGGCA
    TTAAGGGAAC AGGCCCAGTC CTGCAGGCAA CAAGTTGCTC CCACTGATGA TGAAACTGGA
    AAAGAGCTTG TGCTAGCACT CTATGATTAC CAAGAGAAGA GTCCTCGGGA GGTGACTATG
    AAGAAAGGAG ATATTCTAAC CCTGCTCAAC AGCACCAACA AGGACTGGTG GAAGGTTGAA
    GTTAACGATC GTCAGGGCTT TGTACCAGCT GCCTATGTGA AAAAACTAGA TCCTGCCCAG
    TCTGCATCCC GAGAGAATCT TCTAGAAGAG CAAGGCAGCA TAGCATTGCG ACAGGAGCAA
    ATTGACAACC AGTATCATTC CCTTCTTGAG CTGGGAGAAA AACGTAAAGG CATGCTAGAA
    AAAAGCTGCA AGAAGTTTAT GCTTTTCCGT GAGGCTAATG AGCTTCAACA GTGGATCAAT
    GAGAAGGAAG CTGCGCTCAC TAATGAGGAA GTGGGTGCTG ATTTGGAGCA GGTGGAGGTG
    CTACAGAAGA AGTTTGATGA TTTTCAGAAG GATTTAAAAG CTAATGAGTC ACGACTGAAG
    GACATAAACA AGGTTGCAAA TGACCTGGAG TCTGAAGGGC TGATGGCAGA GGAAGTGCAA
    GCAGTAGAGC ACCAGGAAGT CTATGGAATG ATGCCCAGAG ATGAAACTGA TTCTAAGACA
    GCCTCTCCTT GGAAGTCTGC ACGTATGATG GTACACACAG TGGCAACGTT TAACTCAATC
    AAGGAACTGA ATGAACGCTG GAGATCCCTG CAGCAGTTGG CAGAGGAGCG AAGCCAGCTG
    TTGGGCAGCG CTCATGAAGT CCAGAGGTTC CACAGAGATG CTGATGAAAC AAAAGAATGG
    ATAGAAGAGA AGAATCAAGC ATTAAATACC GACAACTATG GACATGACTT GGCCAGTGTG
    CAGGCTCTGC AGCGCAAGCA TGAAGGCTTT GAGAGAGACT TGGCAGCTCT TGGAGACAAG
    GTGAATTCTC TTGGTGAAAC TGCCCAGCGT CTGATCCAGT CACATCCAGA ATCAGCTGAA
    GATCTTCAAG AAAAATGTAC TGAGTTGAAC CAAGCCTGGA GTAGTCTGGG AAAACGTGCT
    GACCAACGCA AAGAGAAGCT TGGAGATTCT CATGACCTGC AGCGTTTCCT CAGTGATTTT
    AGAGACCTTA TGTCTTGGAT CAATGGAATC CGGGGTCTGG TCTCCTCAGA TGAACTTGCA
    AAAGATGTGA CTGGAGCTGA AGCTCTGTTG GAAAGGCATC AGGAGCACCG TACTGAAATA
    GATGCACGGG CTGGCACTTT TCAGGCATTT GAACAGTTTG GACAGCAGCT TCTGGCTCGT
    GGACACTATG CCAGCCCAGA GATTAAGGAG AAACTGGATA TTCTAGATCA AGAACGGACA
    GACCTAGAGA AGGCCTGGGT CCAGCGCAGA ATGATGCTAG ACCAGTGCTT AGAACTACAG
    CTGTTTCATC GGGATTGTGA ACAAGCTGAA AACTGGATGG CTGCCCGAGA GGCGTTCCTA
    AATACAGAAG ACAAAGGAGA CTCCTTAGAC AGCGTGGAGG CACTCATCAA GAAGCATGAA
    GATTTCGATA AAGCAATCAA TGTCCAGGAA GAGAAAATTG CTGTCTTGCA GTCTTTTGCT
    GACCAACTGA TTGCTGCTGA TCATTATGCA AAAGGAGTCA TTGCTAACAG ACGCAATGAG
    GTTCTGGACA GGTGGCGTCG TCTGAAGGCT CAGATGATTG AGAAGAGATC TAAGCTGGGA
    GAATCTCAGA CCCTCCAACA GTTCAGTCGT GATGTTGATG AAATAGAAGC TTGGATCAGT
    GAAAAGCTTC AAACTGCAAG TGATGAGTCA TATAAGGATC CCACAAACAT CCAGAGCAAG
    CACCAGAAGC ACCAAGCCTT TGAAGCTGAG CTCCACGCCA ACGCAGATCG GATTCGTGGA
    GTCATTGAAA TGGGGAACTC TCTTATTGAA AGAGGAGCGT GTGCTGGCAG CGAGGATGCT
    GTGAAGGCTC GGCTGGCTGC CCTGGCTGAC CAATGGGAGT TCCTGGTCCA GAAGTCATCA
    GAGAAGAGTC AGAAACTGAA AGAAGCAAAT AAACAGCAGA ATTTCAATAC CGGCATCAAG
    GACTTTGATT TCTGGCTTTC AGAGGTGGAA GCTTTGTTGG CATCTGAAGA CTATGGGAAG
    GACTTGGCAT CAGTGAACAA CCTTCTGAAG AAGCACCAAT TACTGGAAGC TGATATATCT
    GCTCATGAGG ATCGGCTGAA GGACCTGAAC AGCCAGGCTG ACAGTTTGAT GACCAGCAGT
    GCTTTCGATA CCTCCCAAGT AAAGGATAAA CGTGAAACTA TAAATGGGCG CTTCCAGAGA
    ATCAAGAGCA TGGCAGCTGC CCGCCGTGCG AAGCTCAACG AGTCGCACCG CTTGCATCAG
    TTCTTCCGTG ACATGGATGA TGAGGAGTCC TGGATCAAAG AGAAGAAACT GTTGGTGAGC
    TCAGAGGACT ATGGCAGAGA CCTGACTGGT GTGCAGAACC TGAGGAAGAA ACATAAGCGC
    TTGGAAGCAG AATTAGCTGC CCATGAACCT GCTATCCAGG GTGTTCTAGA CACTGGCAAG
    AAGCTTTCAG ATGATAACAC AATTGGGAAG GAGGAGATAC AGCAAAGACT GGCTCAGTTT
    GTGGACCACT GGAAAGAGTT AAAACAGCTG GCAGCTGCTC GGGGGCAGCG TCTGGAGGAG
    TCCCTGGAGT ACCAACAGTT TGTAGCAAAT GTTGAGGAAG AGGAGGCATG GATCAACGAG
    AAAATGACAT TGGTAGCCAG TGAGGATTAT GGAGACACAC TTGCTGCTAT CCAGGGCTTG
    CTGAAGAAGC ATGAAGCATT CGAGACTGAC TTTACTGTCC ACAAAGACAG AGTGAATGAT
    GTTTGCGCTA ATGGAGAGGA TCTCATTAAA AAGAATAATC ACCATGTGGA GAACATTACT
    GCTAAGATGA AGGGCCTCAA GGGCAAGGTA TCAGATCTGG AGAAAGCCGC AGCTCAGAGG
    AAAGCCAAAC TGGATGAGAA CTCTGCCTTC CTCCAGTTCA ACTGGAAAGC AGATGTGGTG
    GAGTCATGGA TAGGTGAGAA GGAAAACAGT CTGAAGACAG ATGATTATGG CCGTGACCTC
    TCCTCTGTGC AAACACTACT CACCAAACAG GAAACCTTTG ATGCTGGACT TCAGGCTTTC
    CAGCAGGAGG GAATTGCAAA CATCACTGCT CTGAAAGACC AGCTACTGGC AGCCAAACAT
    ATCCAATCAA AGGCCATTGA GGTTCGTCAC GCTTCCTTGA TGAAACGCTG GAATCAGCTG
    CTTGCTAATT CTGCAGCCAG GAAAAAGAAA CTCTTGGAGG CTCAGGAGCA CTTCAGAAAG
    GTTGAAGATC TCTTCTTGAC TTTTGCCAAG AAGGCCTCTG CCTTCAACAG TTGGTTTGAG
    AATGCTGAGG AGGACCTGAC GGATCCCGTG CGCTGCAATT CCCTGGAAGA AATCAAAGCA
    CTGCGAGAAG CCCACGATGC GTTCCGTTCC TCACTTAGCT CTGCCCAAGC TGACTTCAAC
    CAGCTGGCAG AGCTTGATCG GCAGATCAAG AGCTTCCGTG TAGCCTCCAA CCCGTACACT
    TGGTTTACTA TGGAGGCTCT TGAAGAAACT TGGAGGAATC TGCAGAAAAT TATCAAGGAG
    CGTGAATTGG AGTTGCAGAA GGAGCAGCGG AGGCAGGAAG AAAATGACAA GTTGCGCCAG
    GAGTTTGCTC AGCATGCTAA TGCCTTCCAC CAGTGGATTC AGGAGACAAG GACTTACCTG
    CTAGATGGGT CCTGCATGGT GGAGGAGTCG GGAACACTGG AATCCCAGCT GGAAGCTACT
    AAACGCAAGC ACCAGGAGAT CCGGGCTATG AGGAGCCAGC TGAAGAAGAT TGAGGACCTT
    GGAGCAGCCA TGGAAGAGGC ACTTATCTTG GACAACAAAT ACACAGAGCA CAGCACCGTG
    GGGCTGGCGC AGCAGTGGGA CCAGCTTGAC CAGCTGGGAA TGAGAATGCA GCACAATCTG
    GAACAGCAGA TTCAAGCTCG AAACACAACT GGAGTCACAG AGGAGGCCCT GAAGGAGTTC
    AGCATGATGT TCAAGCACTT TGACAAGGAC AAATCTGGAC GTCTTAATCA CCAGGAGTTT
    AAGTCTTGCT TGCGCTCCCT TGGCTATGAC CTGCCCATGG TTGAGGAAGG AGAGCCTGAC
    CCCGAATTTG AGTCTATTCT TGACACTGTT GATCCCAACA GGGATGGCCA CGTCTCGCTG
    CAGGAGTACA TGGCGTTCAT GATCAGCAGG GAAACAGAGA ACGTGAAATC CAGCGAGGAG
    ATTGAGAGTG CTTTCCGTGC CCTCAGCTCG GAGGGGAAGC CTTACGTGAC CAAGGAGGAG
    CTCTACCAGA ACCTGACCCG GGAGCAGGCC GACTACTGCA TTTCTCACAT GAAGCCCTAC
    ATGGATGGCA AGGGCAGAGA ACTTCCTTCT GCCTACGACT ACATAGAATT TACACGTTCA
    CTCTTTGTGA ATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015134780.1
    CDS5..7399
    Translation

    Target ORF information:

    RefSeq Version XM_015279294.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015279294.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
    TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG
    TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC
    TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT
    GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT
    GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG
    ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAA CTCGTCTACA GGAGCTGCAC
    CGACTATGGG AGTTACTGCT GGAAAAAATG AGAGAGAAGG GAGTGAAGCT GTTGCAAGCA
    CAGAAGCTGG TGCAATTCTT ACGGGAATGT GAAGATGTCA TGGACTGGAT CAATGACAAG
    GAAGCAATAG TGACCTCAGA AGAGCTTGGA CAGGACTTAG AGCATGTTGA GGTTTTGCAG
    AAGAAATTTG AAGAGTTCCA AACAGATCTG GCAGCTCATG AGGAGAGAGT GAATGAAGTG
    AACCAGTTCG CTGGCAAACT TATCCAGGAA CAGCACCCTG AGGAGGAACT TATAAAGTCC
    AAACAGGATG AAGTAAATGC AAGCTGGCAG CGTCTTAAGG GGCTTGCCCA ACAGAGGCAA
    GGAAAACTCT TTGGGGCAGC TGAAGTTCAG CGCTTCAACA GGGATGTGGA TGAAACTATC
    AGCTGGATTA AGGAGAAAGG GCAGTTGATG GCCTCAGATG ACTTTGGCAG AGACTTGGCC
    AGCGTGCAAG CTTTACTACG TAAGCATGAA GGCCTGGAAA GAGATCTTGC AGCTTTGGAG
    GATAAGGTTA AGGCCCTCTG TGCAGAAGCT GACCGTTTGC AGCAGTCTCA CCCTATAAAT
    GCTTCTCAAA TTCAAGTGAA ACGGGAGGAA CTGATTGCCA ACTGGGAGCA GATCCGAACT
    CTGGCAGCAG AGAGGCATGC TCGCCTTAAT GACTCCTACA GGTTGCAGCG CTTTCTTGCA
    GATTTCCGGG ACCTCACTAG CTGGGTAACT GAGATGAAGG CTCTGATAAA TGCTGATGAG
    CTTGCCAATG ATGTGGCTGG AGCAGAAGCC CTTCTAGATA GACATCAGGA ACATAAGGGA
    GAAATTGATG CTCATGAGGA TAGCTTCAGA TCTGCTGATG AGTCTGGGCA GGCTTTGCTT
    GCAGCTGGGC ACTATGCTTC TGATGAAGTT AAAGAAAAGC TGACTATCCT CTCAGATGAA
    AGATCAGCCT TGCTGGAACT ATGGGAGCTT CGCAGGCAAC AGTATGAGCA GTGCATGGAT
    CTGCAGCTTT TCTACAGAGA TACTGAACAA GTTGACAACT GGATGAGCAA ACAAGAAGCT
    TTTCTGCTGA ACGAAGACCT TGGTGATTCT CTGGATAGCG TGGAGGCTCT TCTAAAGAAG
    CATGAAGATT TTGAGAAATC CCTAAGTGCT CAAGAGGAGA AGATCACAGC ACTGGATGAG
    TTTGCTACTA AACTGATTCA AAATAACCAT TATGCCATGG ATGACGTTGC TACGCGCAGA
    GATGCTCTTC TGAGTCGCCG AAATGCTCTT CATGAAAGAG CCATGAAGCG CCGTGCCCAA
    CTGGCAGACT CTTTCCATCT CCAGCAGTTT TTCAGGGACT CTGATGAACT GAAGAGTTGG
    GTTAATGAAA AAATGAAAAC TGCTACCGAT GAGGCTTACA AGGATCCATC CAACTTGCAA
    GGTAAAGTTC AGAAGCATCA GGCTTTTGAA GCAGAGCTTT CTGCTAATCA GAGTCGTATT
    GATGCACTGG AGAAAGCTGG CCAGAAGTTG ATTGATGTCA ATCACTATGC ATCTGATGAG
    GTGGCAGCTC GCATGAATGA AGTCATCAGC TTGTGGAAGA AACTTCTGGA AGCCACCGAG
    CTCAAAGGTA TAAAGCTGCG TGAAGCCAAT CAACAGCAGC AATTTAATCG CAATGTAGAA
    GACATTGAGT TGTGGCTGTA TGAAGTGGAG GGTCACTTGG CTTCTGATGA TTATGGAAAA
    GATCTTACCA GCGTTCAGAA TCTTCAGAAG AAACATGCCC TGCTAGAGGC AGATGTTGCT
    GCCCATCAGG ATCGGATAGA TGGCATTACC ATCCAGGCAC GCCAGTTCCA AGATGCTGGG
    CACTTTGATG CTGACAATAT CAAGAAGAAA CAAGAAGCTT TAGTAGCTCG TTATGAAGCT
    CTGAAGGATC CTATGGTGGC TCGCAAGCAG AAACTTGCAG ATTCTCTTCG CCTGCAGCAG
    CTTTTCCGTG ACATCGAGGA TGAAGAGACC TGGATCAGGG AAAAGGAACC TATTGCAGCC
    TCAACAAACC GAGGCAAGGA CTTAATTGGT GTCCAGAATC TGCTAAAGAA GCACCAGGCT
    TTGCAGGCAG AAATTGCAGG CCATGAGCCT CGCATTAAAG CAGTCACACA AAAAGGAAAT
    GCGATGGTGG AAGAGGGACA TTTTGCTGCT GAAGATGTGA AGATCAAATT GAATGAACTA
    AACCAAAAAT GGGACTCTCT GAAAGCCAAG GCATCTCAAC GGCGACAGGA TCTAGAGGAT
    TCTCTGCAAG CTCAGCAGTA TTTTGCTGAT GCTAATGAGG CAGAATCATG GATGAGGGAA
    AAGGAGCCCA TTGTAGGCAG CACGGATTAT GGAAAGGATG AAGACTCTGC TGAGGCTCTC
    CTGAAGAAGC ATGAAGCTTT GATGTCTGAT CTTTCTGCTT ACGGCAGTAG CATACAGGCA
    TTAAGGGAAC AGGCCCAGTC CTGCAGGCAA CAAGTTGCTC CCACTGATGA TGAAACTGGA
    AAAGAGCTTG TGCTAGCACT CTATGATTAC CAAGAGAAGA GTCCTCGGGA GGTGACTATG
    AAGAAAGGAG ATATTCTAAC CCTGCTCAAC AGCACCAACA AGGACTGGTG GAAGGTTGAA
    GTTAACGATC GTCAGGGCTT TGTACCAGCT GCCTATGTGA AAAAACTAGA TCCTGCCCAG
    TCTGCATCCC GAGAGAATCT TCTAGAAGAG CAAGGCAGCA TAGCATTGCG ACAGGAGCAA
    ATTGACAACC AGTATCATTC CCTTCTTGAG CTGGGAGAAA AACGTAAAGG CATGCTAGAA
    AAAAGCTGCA AGAAGTTTAT GCTTTTCCGT GAGGCTAATG AGCTTCAACA GTGGATCAAT
    GAGAAGGAAG CTGCGCTCAC TAATGAGGAA GTGGGTGCTG ATTTGGAGCA GGTGGAGGTG
    CTACAGAAGA AGTTTGATGA TTTTCAGAAG GATTTAAAAG CTAATGAGTC ACGACTGAAG
    GACATAAACA AGGTTGCAAA TGACCTGGAG TCTGAAGGGC TGATGGCAGA GGAAGTGCAA
    GCAGTAGAGC ACCAGGAAGT CTATGGAATG ATGCCCAGAG ATGAAACTGA TTCTAAGACA
    GCCTCTCCTT GGAAGTCTGC ACGTATGATG GTACACACAG TGGCAACGTT TAACTCAATC
    AAGGAACTGA ATGAACGCTG GAGATCCCTG CAGCAGTTGG CAGAGGAGCG AAGCCAGCTG
    TTGGGCAGCG CTCATGAAGT CCAGAGGTTC CACAGAGATG CTGATGAAAC AAAAGAATGG
    ATAGAAGAGA AGAATCAAGC ATTAAATACC GACAACTATG GACATGACTT GGCCAGTGTG
    CAGGCTCTGC AGCGCAAGCA TGAAGGCTTT GAGAGAGACT TGGCAGCTCT TGGAGACAAG
    GTGAATTCTC TTGGTGAAAC TGCCCAGCGT CTGATCCAGT CACATCCAGA ATCAGCTGAA
    GATCTTCAAG AAAAATGTAC TGAGTTGAAC CAAGCCTGGA GTAGTCTGGG AAAACGTGCT
    GACCAACGCA AAGAGAAGCT TGGAGATTCT CATGACCTGC AGCGTTTCCT CAGTGATTTT
    AGAGACCTTA TGTCTTGGAT CAATGGAATC CGGGGTCTGG TCTCCTCAGA TGAACTTGCA
    AAAGATGTGA CTGGAGCTGA AGCTCTGTTG GAAAGGCATC AGGAGCACCG TACTGAAATA
    GATGCACGGG CTGGCACTTT TCAGGCATTT GAACAGTTTG GACAGCAGCT TCTGGCTCGT
    GGACACTATG CCAGCCCAGA GATTAAGGAG AAACTGGATA TTCTAGATCA AGAACGGACA
    GACCTAGAGA AGGCCTGGGT CCAGCGCAGA ATGATGCTAG ACCAGTGCTT AGAACTACAG
    CTGTTTCATC GGGATTGTGA ACAAGCTGAA AACTGGATGG CTGCCCGAGA GGCGTTCCTA
    AATACAGAAG ACAAAGGAGA CTCCTTAGAC AGCGTGGAGG CACTCATCAA GAAGCATGAA
    GATTTCGATA AAGCAATCAA TGTCCAGGAA GAGAAAATTG CTGTCTTGCA GTCTTTTGCT
    GACCAACTGA TTGCTGCTGA TCATTATGCA AAAGGAGTCA TTGCTAACAG ACGCAATGAG
    GTTCTGGACA GGTGGCGTCG TCTGAAGGCT CAGATGATTG AGAAGAGATC TAAGCTGGGA
    GAATCTCAGA CCCTCCAACA GTTCAGTCGT GATGTTGATG AAATAGAAGC TTGGATCAGT
    GAAAAGCTTC AAACTGCAAG TGATGAGTCA TATAAGGATC CCACAAACAT CCAGAGCAAG
    CACCAGAAGC ACCAAGCCTT TGAAGCTGAG CTCCACGCCA ACGCAGATCG GATTCGTGGA
    GTCATTGAAA TGGGGAACTC TCTTATTGAA AGAGGAGCGT GTGCTGGCAG CGAGGATGCT
    GTGAAGGCTC GGCTGGCTGC CCTGGCTGAC CAATGGGAGT TCCTGGTCCA GAAGTCATCA
    GAGAAGAGTC AGAAACTGAA AGAAGCAAAT AAACAGCAGA ATTTCAATAC CGGCATCAAG
    GACTTTGATT TCTGGCTTTC AGAGGTGGAA GCTTTGTTGG CATCTGAAGA CTATGGGAAG
    GACTTGGCAT CAGTGAACAA CCTTCTGAAG AAGCACCAAT TACTGGAAGC TGATATATCT
    GCTCATGAGG ATCGGCTGAA GGACCTGAAC AGCCAGGCTG ACAGTTTGAT GACCAGCAGT
    GCTTTCGATA CCTCCCAAGT AAAGGATAAA CGTGAAACTA TAAATGGGCG CTTCCAGAGA
    ATCAAGAGCA TGGCAGCTGC CCGCCGTGCG AAGCTCAACG AGTCGCACCG CTTGCATCAG
    TTCTTCCGTG ACATGGATGA TGAGGAGTCC TGGATCAAAG AGAAGAAACT GTTGGTGAGC
    TCAGAGGACT ATGGCAGAGA CCTGACTGGT GTGCAGAACC TGAGGAAGAA ACATAAGCGC
    TTGGAAGCAG AATTAGCTGC CCATGAACCT GCTATCCAGG GTGTTCTAGA CACTGGCAAG
    AAGCTTTCAG ATGATAACAC AATTGGGAAG GAGGAGATAC AGCAAAGACT GGCTCAGTTT
    GTGGACCACT GGAAAGAGTT AAAACAGCTG GCAGCTGCTC GGGGGCAGCG TCTGGAGGAG
    TCCCTGGAGT ACCAACAGTT TGTAGCAAAT GTTGAGGAAG AGGAGGCATG GATCAACGAG
    AAAATGACAT TGGTAGCCAG TGAGGATTAT GGAGACACAC TTGCTGCTAT CCAGGGCTTG
    CTGAAGAAGC ATGAAGCATT CGAGACTGAC TTTACTGTCC ACAAAGACAG AGTGAATGAT
    GTTTGCGCTA ATGGAGAGGA TCTCATTAAA AAGAATAATC ACCATGTGGA GAACATTACT
    GCTAAGATGA AGGGCCTCAA GGGCAAGGTA TCAGATCTGG AGAAAGCCGC AGCTCAGAGG
    AAAGCCAAAC TGGATGAGAA CTCTGCCTTC CTCCAGTTCA ACTGGAAAGC AGATGTGGTG
    GAGTCATGGA TAGGTGAGAA GGAAAACAGT CTGAAGACAG ATGATTATGG CCGTGACCTC
    TCCTCTGTGC AAACACTACT CACCAAACAG GAAACCTTTG ATGCTGGACT TCAGGCTTTC
    CAGCAGGAGG GAATTGCAAA CATCACTGCT CTGAAAGACC AGCTACTGGC AGCCAAACAT
    ATCCAATCAA AGGCCATTGA GGTTCGTCAC GCTTCCTTGA TGAAACGCTG GAATCAGCTG
    CTTGCTAATT CTGCAGCCAG GAAAAAGAAA CTCTTGGAGG CTCAGGAGCA CTTCAGAAAG
    GTTGAAGATC TCTTCTTGAC TTTTGCCAAG AAGGCCTCTG CCTTCAACAG TTGGTTTGAG
    AATGCTGAGG AGGACCTGAC GGATCCCGTG CGCTGCAATT CCCTGGAAGA AATCAAAGCA
    CTGCGAGAAG CCCACGATGC GTTCCGTTCC TCACTTAGCT CTGCCCAAGC TGACTTCAAC
    CAGCTGGCAG AGCTTGATCG GCAGATCAAG AGCTTCCGTG TAGCCTCCAA CCCGTACACT
    TGGTTTACTA TGGAGGCTCT TGAAGAAACT TGGAGGAATC TGCAGAAAAT TATCAAGGAG
    CGTGAATTGG AGTTGCAGAA GGAGCAGCGG AGGCAGGAAG AAAATGACAA GTTGCGCCAG
    GAGTTTGCTC AGCATGCTAA TGCCTTCCAC CAGTGGATTC AGGAGACAAG GACTTACCTG
    CTAGATGGGT CCTGCATGGT GGAGGAGTCG GGAACACTGG AATCCCAGCT GGAAGCTACT
    AAACGCAAGC ACCAGGAGAT CCGGGCTATG AGGAGCCAGC TGAAGAAGAT TGAGGACCTT
    GGAGCAGCCA TGGAAGAGGC ACTTATCTTG GACAACAAAT ACACAGAGCA CAGCACCGTG
    GGGCTGGCGC AGCAGTGGGA CCAGCTTGAC CAGCTGGGAA TGAGAATGCA GCACAATCTG
    GAACAGCAGA TTCAAGCTCG AAACACAACT GGAGTCACAG AGGAGGCCCT GAAGGAGTTC
    AGCATGATGT TCAAGCACTT TGACAAGGAC AAATCTGGAC GTCTTAATCA CCAGGAGTTT
    AAGTCTTGCT TGCGCTCCCT TGGCTATGAC CTGCCCATGG TTGAGGAAGG AGAGCCTGAC
    CCCGAATTTG AGTCTATTCT TGACACTGTT GATCCCAACA GGGATGGCCA CGTCTCGCTG
    CAGGAGTACA TGGCGTTCAT GATCAGCAGG GAAACAGAGA ACGTGAAATC CAGCGAGGAG
    ATTGAGAGTG CTTTCCGTGC CCTCAGCTCG GAGGGGAAGC CTTACGTGAC CAAGGAGGAG
    CTCTACCAGA ACCTGACCCG GGAGCAGGCC GACTACTGCA TTTCTCACAT GAAGCCCTAC
    ATGGATGGCA AGGGCAGAGA ACTTCCTTCT GCCTACGACT ACATAGAATT TACACGTTCA
    CTCTTTGTGA ATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa27423
    Clone ID Related Accession (Same CDS sequence) NM_001042538.1
    Accession Version NM_001042538.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7434bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-22
    Organism Gallus gallus(chicken)
    Product spectrin alpha chain, non-erythrocytic 1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CN217639.1 and X14519.1. On Jul 14, 2006 this sequence version replaced XM_415495.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGATCCAA GTGGGGTAAA AGTGTTGGAA ACAGCAGAAG ATATCCAAGA ACGGCGTCAG 
    CAGGTTTTGG ACCGTTACCA CAGGTTCAAG GAGCTGTCTT CTCTGAGGCG CCAAAAACTC
    GAAGATTCCT ATCGGTTCCA GTTCTTCCAG CGTGATGCAG ATGAGCTGGG AAAATGGATC
    CAAGAGAAAC TCCAGATAGC ATCTGATGAA AATTACAAAG ACCCAAGCAA TTTACAGGGG
    AAGCTGCAGA AGCACCAGGC CTTTGAAGCT GAGGTGCAGG CCAATTCAGG GGCTATCGTT
    AAGCTGGATG AGACTGGAAA TCAGATGATC AATGAAGGCC ATTTTGCATC TGAAACCATA
    AGAACTCGTC TACAGGAGCT GCACCGACTA TGGGAGTTAC TGCTGGAAAA AATGAGAGAG
    AAGGGAGTGA AGCTGTTGCA AGCACAGAAG CTGGTGCAAT TCTTACGGGA ATGTGAAGAT
    GTCATGGACT GGATCAATGA CAAGGAAGCA ATAGTGACCT CAGAAGAGCT TGGACAGGAC
    TTAGAGCATG TTGAGGTTTT GCAGAAGAAA TTTGAAGAGT TCCAAACAGA TCTGGCAGCT
    CATGAGGAGA GAGTGAATGA AGTGAACCAG TTCGCTGGGA AACTTATCCA GGAACAGCAC
    CCTGAGGAGG AACTTATAAA GTCCAAACAG GATGAAGTAA ATGCAAGCTG GCAGCGTCTT
    AAGGGGCTTG CCCAACAGAG GCAAGGAAAA CTCTTTGGGG CAGCTGAAGT TCAGCGCTTC
    AACAGGGATG TGGATGAAAC TATCAGCTGG ATTAAGGAGA AAGGGCAGTT GATGGCCTCA
    GATGACTTTG GCAGAGACTT GGCCAGCGTG CAAGCTTTAC TACGTAAGCA TGAAGGCCTG
    GAAAGAGATC TTGCAGCTTT CCACCATAAG GTTAAGGCCC TCTGTGCAGA AGCTGACCGT
    TTGCAGCAAT CTCACCCTAT AAATGCTTCT CAAATTCAAG TGAAACGGGA GGAACTGATT
    GCCAACTGGG AGCAGATCCG AACTCTGGCA GCAGAGAGGC ATGCTCGCCT TAATGACTCC
    TACAGGTTGC AGCGCTTTCT TGCAGATTTC CGGGACCTCA CTAGCTGGGT AACTGAGATG
    AAGGCTCTGA TAAATGCTGA TGAGCTTGCC AATGATGTGG CTGGAGCAGA AGCCCTTCTA
    GATAGACATC AGGAACATAA GGGAGAAATT GATGCTCATG AGGATAGCTT CAGATCTGCT
    GATGAGTCTG GGCAGGCTTT GCTTGCAGCT GGGCACTATG CTTCTGATGA AGTTAAAGAA
    AAGCTGACTA TCCTCTCAGA TGAAAGATCT GCCTTGCTGG AACTATGGGA GCTTCGCAGG
    CAACAGTATG AGCAGTGCAT GGATCTGCAG CTTTTCTACA GAGATACTGA ACAAGTTGAC
    AACTGGATGA GCAAACAAGA AGCTTTTCTG CTGAACGAAG ACCTTGGTGA TTCTCTGGAT
    AGCGTGGAGG CTCTTCTAAA GAAGCATGAA GATTTTGAGA AATCCCTAAG TGCTCAAGAG
    GAGAAAATCA CAGCACTGGA TGAGTTTGCT ACTAAACTGA TTCAAAATAA CCATTATGCC
    ATGGATGACG TTGCTACGCG CAGAGATGCT CTTCTGAGTC GCCGAAATGC TCTTCATGAA
    AGAGCCATGA AGCGCCGTGC CCAACTGGCA GACTCTTTCC ATCTCCAGCA GTTTTTCAGG
    GACTCTGATG AACTGAAGAG TTGGGTTAAT GAAAAAATGA AAACTGCTAC CGATGAGGCT
    TACAAGGATC CATCCAACTT GCAAGGTAAA GTTCAGAAGC ATCAGGCTTT TGAAGCAGAG
    CTTTCTGCTA ATCAGAGTCG TATTGATGCA CTGGAGAAAG CTGGCCAGAA GTTGATTGAT
    GTCAATCACT ATGCATCTGA TGAGGTGGCA GCTCGCATGA ATGAAGTCAT CAGCTTGTGG
    AAGAAACTTC TGGAAGCCAC CGAGCTCAAA GGTATAAAGC TGCGTGAAGC CAATCAACAG
    CAGCAATTTA ATCGCAATGT AGAAGACATT GAGTTGTGGC TGTATGAAGT GGAGGGTCAC
    TTGGCTTCTG ATGATTATGG AAAAGATCTT ACCAGCGTTC AGAATCTTCA GAAGAAACAT
    GCCCTGCTAG AGGCAGATGT TGCTGCCCAT CAGGATCCGA TAGATGGCAT TACCATCCAG
    GCACGCCAGT TCCAAGATGC TGGGCACTTT GATGCTGACA ATATCAAGAA GAAACAAGAA
    GCTTTAGTAG CTCGTTATGA AGCTCTGAAG GATCCTATGG TGGCTCGCAA GCAGAAACTT
    GCAGATTCTC TTCGCCTGCA GCAGCTTTTC CGTGACATCG AGGATGAAGA GACCTGGATC
    AGGGAAAAGG AACCTATTGC AGCCTCAACA AACCGAGGCA AGGACTTAAT TGGTGTCCAG
    AATCTGCTAA AGAAGCACCA GGCTTTGCAG GCAGAAATTG CAGGCCATGA GCCTCGCATT
    AAAGCAGTCA CACAAAAAGG AAATGCGATG GTGGAAGAGG GACATTTTGC TGCTGAAGAT
    GTGAAGATCA AATTGAATGA ACTAAACCAA AAATGGGACT CTCTGAAAGC GAAGGCATCT
    CAACGGCGAC AGGATCTAGA GGATTCTCTG CAAGCTCAGC AGTATTTTGC TGATGCTAAT
    GAGGCACAAT CATGGATGAG GGAAAAGGAG CCCATTGTAG GCAGCACGGA TTATGGAAAG
    GATGAAGACT CTGCTGAGGC TCTCCTGAAG AAGCATGAAG CTTTGATGTC TGATCTTTCT
    GCTTACGGCA GTAGCATACA GGCATTAAGG GAACAGGCCC AGTCCTGCAG GCAACAAGTT
    GCTCCCACTG ATGATGAAAC TGGAAAAGAG CTTGTGCTAG CACTCTATGA TTACCAAGAG
    AAGAGTCCTC GGGAGGTGAC TATGAAGAAA GGAGATATTC TAACCCTGCT CAACAGCACC
    AACAAGGACT GGTGGAAGGT TGAAGTTAAC GATCGTCAGG GCTTTGTACC AGCTGCCTAT
    GTGAAAAAAC TAGATCCTGC CCAGTCTGCA TCCCGAGAGA ATCTTCTGGA AGAGCAAGGC
    AGCATAGCAT TGCGACAGGA GCAAATTGAC AACCAGACTC TCATTACTAA GGAAGTCGGC
    AGTGTATCTC TGCGTATGAA ACAGGTCGAA GAACTGTATC ATTCCCTTCT TGAGCTGGGA
    GAAAAACGTA AAGGCATGCT AGAAAAAAGC TGCAAGAAGT TTATGCTTTT CCGTGAGGCT
    AATGAGCTTC AACAGTGGAT CAATGAGAAG GAAGCTGCAC TCACTAACGA GGAAGTGGGT
    GCTGATTTGG AGCAGGTGGA GGTGCTACAG AAGAAGTTTG ATGATTTTCA GAAGGATTTA
    AAAGCTAATG AGTCACGACT GAAGGACATA AACAAGGTTG CAAATGATCT GGAGTCTGAA
    GGGCTGATGG CAGAGGAAGT GCAAGCAGTA GAGCACCAGG AAGTCTATGG AATGATGCCC
    AGAGATGAAA CTGATTCTAA GACAGTCTCT CCTTGGAAGT CTGCACGTAT GATGGTACAC
    ACGGTGGCAA CATTTAACTC AATTAAGGAA CTGAATGAAC GCTGGAGATC CCTGCAGCAG
    TTGGCAGAGG AGCGAAGCCA GCTGTTGGGC AGCGCTGATG AAGTCCAGAG GTTCCACAGA
    GATGCTGATG AAACAAAAGA ATGGATAGAA GAGAAGAATC AAGCATTAAA TACCGACAAC
    TATGGACATG ACTTGGCCAG TGTGCAGGCT CTGCAGCGCA ACGATGAAGG CTTTGAGAGA
    GACTTGGCAG CTCTTGGAGA CAAGGTGAAT TCTCTTGGTG AAACTGCCCA GCGTCTGATC
    CAGTCACATC CAGAACTAGC TGAAGATCTT CAAGAAAAAT GTACTGAGTT GAACCAAGCC
    TGGAGTAGTC TGGGAAAACG TGCTGACCAA CGCAAAGAGA AGCTTGGAGA TTCTCATGAC
    CTGCAGCGTT TCCTCAGTGA TTTTAGAGAC CTTATGTCTT GGATCAATGG AATCCGGGGT
    CTGGTCTCCT CAGATGAACT TGCAAAAGAT GTGACTGGAG CTGAAGCTCT GTTGGAAAGG
    CATCAGGAGC ACCGTACTGA AATAGATGCA CGGGCTGGCA CTTTTCAGGC ATTTGAACAG
    TTTGGACAGC AGCTTCTGGC TCGTGGACAC TATGCCAGCC CAGAGATTAA GGAGAAACTG
    GATATTCTAG ATCAAGAACG GACAGACCTA GAGAAGGCCT GGGTCCAGCG CAGAATGATG
    CTAGACCAGT GCTTAGAACT ACAGCTGTTT CATCGGGATT GTGAACAAGC TGAAAACTGG
    ATGGCTGCCC GAGAGGCGTT CCTAAATACA GAAGACAAAG GAGACTCCTT AGACAGCGTG
    GAGGCACTCA TCAAGAAGCA TGAAGATTTC GATAAAGCAA TCAATGTCCA GGAAGAGAAA
    ATTGCTGTCT TGCAGTCTTT TGCTGACCAA CTGATTGCTG CTGATCATTA TGCAAAAGGA
    GTCATTGCTA ACAGACGCAA TGAGGTTCTG GACAGGTGGC GTCGTCTGAA GGCTCAGATG
    ATTGAGAAGA GATCTAAGCT GGGAGAATCT CAGACCCTCC AACAGTTCAG TCGTGATGTT
    GATGAAATAG AAGCTTGGAT CAGTGAAAAG CTTCAAACTG CAAGTGATGA GTCATATAAG
    GATCCCACAA ACATCCAGCT TTCCAAACTG CTGAGCAAGC ACCAGAAGCA CCAAGCCTTT
    GAAGCTGAGC TCCACGCCAA CGCAGATCGG ATTCGTGGAG TCATTGAAAT GGGGAACCCT
    CTTATTGAAA GAGGAGCGTG TGCTGGCAGC GAGGATGCTG TGAAGGCTCG GCTGGCTGCC
    CTGGCTGACC AATGGGAGTT CCTGGTCCAG AAGTCATCAG AGAAGAGTCA GAAACTGAAA
    GAAGCAAATA AACAGCAGAA TTTCAATACC GGCATCAAGG ACTTTGATTT CTGGCTTTCA
    GAGGTGGAAG CTTTGTTGGC ATCTGAAGAC TATGGGAAGG ACTTGGCATC AGTGAACAAC
    CTTCTGAAGA AGCACCAATT ACTGGAAGCT GATATATCTG CTCATGAGGA TCGGCTGAAG
    GACCTGAACA GCCAGGCTGA CAGTTTGATG ACCAGCAGTG CTTTCGATAC CTCCCAAGTA
    AAGGATAAAC GTGAAACTAT AAATGGGCGC TTCCAGAGAA TCAAGAGCAT GGCAGCTGCC
    CGCCGTGCGA AGCTCAACGA GTCGCACCGC TTGCATCAGT TCTTCCGTGA CATGGATGAT
    GAGGAGTCCT GGATCAAAGA GAAGAAACTG TTGGTGAGCT CAGAGGACTA TGGCAGAGAC
    CTGACTGGTG TGCAGAACCT GAGGAAGAAA CATAAGCGCT TGGAAGCAGA ATTAGCTGCC
    CATGAACCTG CTATCCAGGG TGTTCTAGAC ACTGGCAAGA AGCTTTCAGA TGATAACACA
    ATTGGGAAGG AGGAGATACA GCAAAGACTG GCTCAGTTTG TGGACCACTG GAAAGAGTTA
    AAACAGCTGG CAGCTGCTCG GGGGCAACGT CTGGAGGAGT CCCTGGAGTA CCAACAGTTT
    GTAGCAAATG TTGAGGAAGA GGAGGCATGG ATCAACGAGA AAATGACATT GGTAGCCAGT
    GAGGATTATG GAGACACACT TGCTGCTATC CAGGGCTTGC TGAAGAAGCA TGAAGCATTC
    GAGACTGACT TTACTGTCCA CAAAGACAGA GTGAATGATG TTTGTGCTAA TGGAGAGGAT
    CTCATTAAAA AGAATAATCA CCATGTGGAG AACATTACTG CTAAGATGAA GGGCCTCAAG
    GGCAAGGTAT CAGATCTGGA GAAAGCCGCA GCTCAGAGGA AAGCCAAACT GGATGAGAAC
    TCTGCCTTCC TCCAGTTCAA CTGGAAAGCA GATGTGGTGG AGTCATGGAT AGGTGAGAAG
    GAAAACAGCC TGAAGACAGA TGATTATGGC CGTGACCTCT CCTCTGTGCA AACACTACTC
    ACCAAACAGG AAACCTTTGA TGCTGGACTT CAGGCTTTCC AGCAGGAGGG AATTGCAAAC
    ATCACTGCTC TGAAAGACCA GCTACTGGCA GCCAAACATA TCCAATCAAA GGCCATTGAG
    GTTCGTCACG CTTCCTTGAT GAAACGCTGG AATCAGCTGC TTGCTAATTC TGCAGCCAGG
    AAAAAGAAAC TCTTGGAGGC TCAGGAGCAC TTCAGAAAGG TTGAAGATCT CTTCTTGACT
    TTTGCCAAGA AGGCCTCTGC CTTCAACAGT TGGTTTGAGA ATGCTGAGGA GGACCTGACG
    GATCCCGTGC GCTGCAATTC CCTGGAAGAA ATCAAAGCAC TGCGAGAAGC CCACGATGCC
    TTCCGTTCCT CACTTAGCTC TGCCCAAGCT GACTTCAACC AGCTGGCAGA GCTTGATCGG
    CAGATCAAGA GCTTCCGTGT AGCCTCCAAC CCGTACACTT GGTTTACTAT GGAGGCTCTT
    GAAGAAACTT GGAGGAATCT GCAGAAAATT ATCAAGGAGC GTGAATTGGA GTTGCAGAAG
    GAGCAGCGGA GGCAGGAAGA AAATGACAAG TTGCGCCAGG AGTTTGCTCA GCATGCTAAT
    GCCTTCCACC AGTGGATTCA GGAGACAAGG ACTTACCTGC TAGATGGGTC CTGTATGGTG
    GAGGAGTCGG GAACACTGGA ATCCCAGCTG GAAGCTACTA AACGCAAGCA CCAGGAGATC
    CGGGCTATGA GGAGCCAGCT GAAGAAGATT GAGGACCTTG GAGCAGCCAT GGAAGAGGCA
    CTTATCTTGG ACAACAAATA CACAGAGCAC AGCACCGTGG GGCTGGCGCA GCAGTGGGAC
    CAGCTTGACC AGCTGGGAAT GAGAATGCAG CACAATCTGG AACAGCAGAT TCAAGCTCGA
    AACACAACTG GAGTCACAGA GGAGGCCCTG AAGGAGTTCA GCATGATGTT CAAGCACTTT
    GACAAGGACA AATCTGGACG TCTTAATCAC CAGGAGTTTA AGTCTTGCTT GCGCTCCCTT
    GGCTATGACC TGCCCATGGT TGAGGAAGGA GAGCCTGACC CCGAATTTGA GTCTATTCTT
    GACACTGTTG ATCCCAACAG GGATGGCCAC GTCTCGCTGC AGGAGTACAT GGCGTTCATG
    ATCAGCAGGG AAACAGAGAA CGTGAAATCC AGCGAGGAGA TTGAGAGTGC TTTCCGTGCC
    CTCAGCTCGG AGAGGAAGCC TTACGTGACC AAGGAGGAGC TCTACCAGAA CCTGACCCGG
    GAGCAGGCCG ACTACTGCAT TTCTCACATG AAGCCCTACA TGGATGGCAA GGGCAGAGAA
    CTTCCTTCTG CCTACGACTA CATAGAATTT ACACGTTCAC TCTTTGTGAA TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001036003.1
    CDS20..7453
    Translation

    Target ORF information:

    RefSeq Version NM_001042538.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001042538.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGATCCAA GTGGGGTAAA AGTGTTGGAA ACAGCAGAAG ATATCCAAGA ACGGCGTCAG 
    CAGGTTTTGG ACCGTTACCA CAGGTTCAAG GAGCTGTCTT CTCTGAGGCG CCAAAAACTC
    GAAGATTCCT ATCGGTTCCA GTTCTTCCAG CGTGATGCAG ATGAGCTGGG AAAATGGATC
    CAAGAGAAAC TCCAGATAGC ATCTGATGAA AATTACAAAG ACCCAAGCAA TTTACAGGGG
    AAGCTGCAGA AGCACCAGGC CTTTGAAGCT GAGGTGCAGG CCAATTCAGG GGCTATCGTT
    AAGCTGGATG AGACTGGAAA TCAGATGATC AATGAAGGCC ATTTTGCATC TGAAACCATA
    AGAACTCGTC TACAGGAGCT GCACCGACTA TGGGAGTTAC TGCTGGAAAA AATGAGAGAG
    AAGGGAGTGA AGCTGTTGCA AGCACAGAAG CTGGTGCAAT TCTTACGGGA ATGTGAAGAT
    GTCATGGACT GGATCAATGA CAAGGAAGCA ATAGTGACCT CAGAAGAGCT TGGACAGGAC
    TTAGAGCATG TTGAGGTTTT GCAGAAGAAA TTTGAAGAGT TCCAAACAGA TCTGGCAGCT
    CATGAGGAGA GAGTGAATGA AGTGAACCAG TTCGCTGGGA AACTTATCCA GGAACAGCAC
    CCTGAGGAGG AACTTATAAA GTCCAAACAG GATGAAGTAA ATGCAAGCTG GCAGCGTCTT
    AAGGGGCTTG CCCAACAGAG GCAAGGAAAA CTCTTTGGGG CAGCTGAAGT TCAGCGCTTC
    AACAGGGATG TGGATGAAAC TATCAGCTGG ATTAAGGAGA AAGGGCAGTT GATGGCCTCA
    GATGACTTTG GCAGAGACTT GGCCAGCGTG CAAGCTTTAC TACGTAAGCA TGAAGGCCTG
    GAAAGAGATC TTGCAGCTTT CCACCATAAG GTTAAGGCCC TCTGTGCAGA AGCTGACCGT
    TTGCAGCAAT CTCACCCTAT AAATGCTTCT CAAATTCAAG TGAAACGGGA GGAACTGATT
    GCCAACTGGG AGCAGATCCG AACTCTGGCA GCAGAGAGGC ATGCTCGCCT TAATGACTCC
    TACAGGTTGC AGCGCTTTCT TGCAGATTTC CGGGACCTCA CTAGCTGGGT AACTGAGATG
    AAGGCTCTGA TAAATGCTGA TGAGCTTGCC AATGATGTGG CTGGAGCAGA AGCCCTTCTA
    GATAGACATC AGGAACATAA GGGAGAAATT GATGCTCATG AGGATAGCTT CAGATCTGCT
    GATGAGTCTG GGCAGGCTTT GCTTGCAGCT GGGCACTATG CTTCTGATGA AGTTAAAGAA
    AAGCTGACTA TCCTCTCAGA TGAAAGATCT GCCTTGCTGG AACTATGGGA GCTTCGCAGG
    CAACAGTATG AGCAGTGCAT GGATCTGCAG CTTTTCTACA GAGATACTGA ACAAGTTGAC
    AACTGGATGA GCAAACAAGA AGCTTTTCTG CTGAACGAAG ACCTTGGTGA TTCTCTGGAT
    AGCGTGGAGG CTCTTCTAAA GAAGCATGAA GATTTTGAGA AATCCCTAAG TGCTCAAGAG
    GAGAAAATCA CAGCACTGGA TGAGTTTGCT ACTAAACTGA TTCAAAATAA CCATTATGCC
    ATGGATGACG TTGCTACGCG CAGAGATGCT CTTCTGAGTC GCCGAAATGC TCTTCATGAA
    AGAGCCATGA AGCGCCGTGC CCAACTGGCA GACTCTTTCC ATCTCCAGCA GTTTTTCAGG
    GACTCTGATG AACTGAAGAG TTGGGTTAAT GAAAAAATGA AAACTGCTAC CGATGAGGCT
    TACAAGGATC CATCCAACTT GCAAGGTAAA GTTCAGAAGC ATCAGGCTTT TGAAGCAGAG
    CTTTCTGCTA ATCAGAGTCG TATTGATGCA CTGGAGAAAG CTGGCCAGAA GTTGATTGAT
    GTCAATCACT ATGCATCTGA TGAGGTGGCA GCTCGCATGA ATGAAGTCAT CAGCTTGTGG
    AAGAAACTTC TGGAAGCCAC CGAGCTCAAA GGTATAAAGC TGCGTGAAGC CAATCAACAG
    CAGCAATTTA ATCGCAATGT AGAAGACATT GAGTTGTGGC TGTATGAAGT GGAGGGTCAC
    TTGGCTTCTG ATGATTATGG AAAAGATCTT ACCAGCGTTC AGAATCTTCA GAAGAAACAT
    GCCCTGCTAG AGGCAGATGT TGCTGCCCAT CAGGATCCGA TAGATGGCAT TACCATCCAG
    GCACGCCAGT TCCAAGATGC TGGGCACTTT GATGCTGACA ATATCAAGAA GAAACAAGAA
    GCTTTAGTAG CTCGTTATGA AGCTCTGAAG GATCCTATGG TGGCTCGCAA GCAGAAACTT
    GCAGATTCTC TTCGCCTGCA GCAGCTTTTC CGTGACATCG AGGATGAAGA GACCTGGATC
    AGGGAAAAGG AACCTATTGC AGCCTCAACA AACCGAGGCA AGGACTTAAT TGGTGTCCAG
    AATCTGCTAA AGAAGCACCA GGCTTTGCAG GCAGAAATTG CAGGCCATGA GCCTCGCATT
    AAAGCAGTCA CACAAAAAGG AAATGCGATG GTGGAAGAGG GACATTTTGC TGCTGAAGAT
    GTGAAGATCA AATTGAATGA ACTAAACCAA AAATGGGACT CTCTGAAAGC GAAGGCATCT
    CAACGGCGAC AGGATCTAGA GGATTCTCTG CAAGCTCAGC AGTATTTTGC TGATGCTAAT
    GAGGCACAAT CATGGATGAG GGAAAAGGAG CCCATTGTAG GCAGCACGGA TTATGGAAAG
    GATGAAGACT CTGCTGAGGC TCTCCTGAAG AAGCATGAAG CTTTGATGTC TGATCTTTCT
    GCTTACGGCA GTAGCATACA GGCATTAAGG GAACAGGCCC AGTCCTGCAG GCAACAAGTT
    GCTCCCACTG ATGATGAAAC TGGAAAAGAG CTTGTGCTAG CACTCTATGA TTACCAAGAG
    AAGAGTCCTC GGGAGGTGAC TATGAAGAAA GGAGATATTC TAACCCTGCT CAACAGCACC
    AACAAGGACT GGTGGAAGGT TGAAGTTAAC GATCGTCAGG GCTTTGTACC AGCTGCCTAT
    GTGAAAAAAC TAGATCCTGC CCAGTCTGCA TCCCGAGAGA ATCTTCTGGA AGAGCAAGGC
    AGCATAGCAT TGCGACAGGA GCAAATTGAC AACCAGACTC TCATTACTAA GGAAGTCGGC
    AGTGTATCTC TGCGTATGAA ACAGGTCGAA GAACTGTATC ATTCCCTTCT TGAGCTGGGA
    GAAAAACGTA AAGGCATGCT AGAAAAAAGC TGCAAGAAGT TTATGCTTTT CCGTGAGGCT
    AATGAGCTTC AACAGTGGAT CAATGAGAAG GAAGCTGCAC TCACTAACGA GGAAGTGGGT
    GCTGATTTGG AGCAGGTGGA GGTGCTACAG AAGAAGTTTG ATGATTTTCA GAAGGATTTA
    AAAGCTAATG AGTCACGACT GAAGGACATA AACAAGGTTG CAAATGATCT GGAGTCTGAA
    GGGCTGATGG CAGAGGAAGT GCAAGCAGTA GAGCACCAGG AAGTCTATGG AATGATGCCC
    AGAGATGAAA CTGATTCTAA GACAGTCTCT CCTTGGAAGT CTGCACGTAT GATGGTACAC
    ACGGTGGCAA CATTTAACTC AATTAAGGAA CTGAATGAAC GCTGGAGATC CCTGCAGCAG
    TTGGCAGAGG AGCGAAGCCA GCTGTTGGGC AGCGCTGATG AAGTCCAGAG GTTCCACAGA
    GATGCTGATG AAACAAAAGA ATGGATAGAA GAGAAGAATC AAGCATTAAA TACCGACAAC
    TATGGACATG ACTTGGCCAG TGTGCAGGCT CTGCAGCGCA ACGATGAAGG CTTTGAGAGA
    GACTTGGCAG CTCTTGGAGA CAAGGTGAAT TCTCTTGGTG AAACTGCCCA GCGTCTGATC
    CAGTCACATC CAGAACTAGC TGAAGATCTT CAAGAAAAAT GTACTGAGTT GAACCAAGCC
    TGGAGTAGTC TGGGAAAACG TGCTGACCAA CGCAAAGAGA AGCTTGGAGA TTCTCATGAC
    CTGCAGCGTT TCCTCAGTGA TTTTAGAGAC CTTATGTCTT GGATCAATGG AATCCGGGGT
    CTGGTCTCCT CAGATGAACT TGCAAAAGAT GTGACTGGAG CTGAAGCTCT GTTGGAAAGG
    CATCAGGAGC ACCGTACTGA AATAGATGCA CGGGCTGGCA CTTTTCAGGC ATTTGAACAG
    TTTGGACAGC AGCTTCTGGC TCGTGGACAC TATGCCAGCC CAGAGATTAA GGAGAAACTG
    GATATTCTAG ATCAAGAACG GACAGACCTA GAGAAGGCCT GGGTCCAGCG CAGAATGATG
    CTAGACCAGT GCTTAGAACT ACAGCTGTTT CATCGGGATT GTGAACAAGC TGAAAACTGG
    ATGGCTGCCC GAGAGGCGTT CCTAAATACA GAAGACAAAG GAGACTCCTT AGACAGCGTG
    GAGGCACTCA TCAAGAAGCA TGAAGATTTC GATAAAGCAA TCAATGTCCA GGAAGAGAAA
    ATTGCTGTCT TGCAGTCTTT TGCTGACCAA CTGATTGCTG CTGATCATTA TGCAAAAGGA
    GTCATTGCTA ACAGACGCAA TGAGGTTCTG GACAGGTGGC GTCGTCTGAA GGCTCAGATG
    ATTGAGAAGA GATCTAAGCT GGGAGAATCT CAGACCCTCC AACAGTTCAG TCGTGATGTT
    GATGAAATAG AAGCTTGGAT CAGTGAAAAG CTTCAAACTG CAAGTGATGA GTCATATAAG
    GATCCCACAA ACATCCAGCT TTCCAAACTG CTGAGCAAGC ACCAGAAGCA CCAAGCCTTT
    GAAGCTGAGC TCCACGCCAA CGCAGATCGG ATTCGTGGAG TCATTGAAAT GGGGAACCCT
    CTTATTGAAA GAGGAGCGTG TGCTGGCAGC GAGGATGCTG TGAAGGCTCG GCTGGCTGCC
    CTGGCTGACC AATGGGAGTT CCTGGTCCAG AAGTCATCAG AGAAGAGTCA GAAACTGAAA
    GAAGCAAATA AACAGCAGAA TTTCAATACC GGCATCAAGG ACTTTGATTT CTGGCTTTCA
    GAGGTGGAAG CTTTGTTGGC ATCTGAAGAC TATGGGAAGG ACTTGGCATC AGTGAACAAC
    CTTCTGAAGA AGCACCAATT ACTGGAAGCT GATATATCTG CTCATGAGGA TCGGCTGAAG
    GACCTGAACA GCCAGGCTGA CAGTTTGATG ACCAGCAGTG CTTTCGATAC CTCCCAAGTA
    AAGGATAAAC GTGAAACTAT AAATGGGCGC TTCCAGAGAA TCAAGAGCAT GGCAGCTGCC
    CGCCGTGCGA AGCTCAACGA GTCGCACCGC TTGCATCAGT TCTTCCGTGA CATGGATGAT
    GAGGAGTCCT GGATCAAAGA GAAGAAACTG TTGGTGAGCT CAGAGGACTA TGGCAGAGAC
    CTGACTGGTG TGCAGAACCT GAGGAAGAAA CATAAGCGCT TGGAAGCAGA ATTAGCTGCC
    CATGAACCTG CTATCCAGGG TGTTCTAGAC ACTGGCAAGA AGCTTTCAGA TGATAACACA
    ATTGGGAAGG AGGAGATACA GCAAAGACTG GCTCAGTTTG TGGACCACTG GAAAGAGTTA
    AAACAGCTGG CAGCTGCTCG GGGGCAACGT CTGGAGGAGT CCCTGGAGTA CCAACAGTTT
    GTAGCAAATG TTGAGGAAGA GGAGGCATGG ATCAACGAGA AAATGACATT GGTAGCCAGT
    GAGGATTATG GAGACACACT TGCTGCTATC CAGGGCTTGC TGAAGAAGCA TGAAGCATTC
    GAGACTGACT TTACTGTCCA CAAAGACAGA GTGAATGATG TTTGTGCTAA TGGAGAGGAT
    CTCATTAAAA AGAATAATCA CCATGTGGAG AACATTACTG CTAAGATGAA GGGCCTCAAG
    GGCAAGGTAT CAGATCTGGA GAAAGCCGCA GCTCAGAGGA AAGCCAAACT GGATGAGAAC
    TCTGCCTTCC TCCAGTTCAA CTGGAAAGCA GATGTGGTGG AGTCATGGAT AGGTGAGAAG
    GAAAACAGCC TGAAGACAGA TGATTATGGC CGTGACCTCT CCTCTGTGCA AACACTACTC
    ACCAAACAGG AAACCTTTGA TGCTGGACTT CAGGCTTTCC AGCAGGAGGG AATTGCAAAC
    ATCACTGCTC TGAAAGACCA GCTACTGGCA GCCAAACATA TCCAATCAAA GGCCATTGAG
    GTTCGTCACG CTTCCTTGAT GAAACGCTGG AATCAGCTGC TTGCTAATTC TGCAGCCAGG
    AAAAAGAAAC TCTTGGAGGC TCAGGAGCAC TTCAGAAAGG TTGAAGATCT CTTCTTGACT
    TTTGCCAAGA AGGCCTCTGC CTTCAACAGT TGGTTTGAGA ATGCTGAGGA GGACCTGACG
    GATCCCGTGC GCTGCAATTC CCTGGAAGAA ATCAAAGCAC TGCGAGAAGC CCACGATGCC
    TTCCGTTCCT CACTTAGCTC TGCCCAAGCT GACTTCAACC AGCTGGCAGA GCTTGATCGG
    CAGATCAAGA GCTTCCGTGT AGCCTCCAAC CCGTACACTT GGTTTACTAT GGAGGCTCTT
    GAAGAAACTT GGAGGAATCT GCAGAAAATT ATCAAGGAGC GTGAATTGGA GTTGCAGAAG
    GAGCAGCGGA GGCAGGAAGA AAATGACAAG TTGCGCCAGG AGTTTGCTCA GCATGCTAAT
    GCCTTCCACC AGTGGATTCA GGAGACAAGG ACTTACCTGC TAGATGGGTC CTGTATGGTG
    GAGGAGTCGG GAACACTGGA ATCCCAGCTG GAAGCTACTA AACGCAAGCA CCAGGAGATC
    CGGGCTATGA GGAGCCAGCT GAAGAAGATT GAGGACCTTG GAGCAGCCAT GGAAGAGGCA
    CTTATCTTGG ACAACAAATA CACAGAGCAC AGCACCGTGG GGCTGGCGCA GCAGTGGGAC
    CAGCTTGACC AGCTGGGAAT GAGAATGCAG CACAATCTGG AACAGCAGAT TCAAGCTCGA
    AACACAACTG GAGTCACAGA GGAGGCCCTG AAGGAGTTCA GCATGATGTT CAAGCACTTT
    GACAAGGACA AATCTGGACG TCTTAATCAC CAGGAGTTTA AGTCTTGCTT GCGCTCCCTT
    GGCTATGACC TGCCCATGGT TGAGGAAGGA GAGCCTGACC CCGAATTTGA GTCTATTCTT
    GACACTGTTG ATCCCAACAG GGATGGCCAC GTCTCGCTGC AGGAGTACAT GGCGTTCATG
    ATCAGCAGGG AAACAGAGAA CGTGAAATCC AGCGAGGAGA TTGAGAGTGC TTTCCGTGCC
    CTCAGCTCGG AGAGGAAGCC TTACGTGACC AAGGAGGAGC TCTACCAGAA CCTGACCCGG
    GAGCAGGCCG ACTACTGCAT TTCTCACATG AAGCCCTACA TGGATGGCAA GGGCAGAGAA
    CTTCCTTCTG CCTACGACTA CATAGAATTT ACACGTTCAC TCTTTGTGAA TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.