SPTAN1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPTAN1
Entrez Gene ID	6709
Full Name	spectrin alpha, non-erythrocytic 1
Synonyms	EIEE5,NEAS,SPTA2
General protein information	Preferred Names spectrin alpha, non-erythrocytic 1 Names spectrin alpha chain, non-erythrocytic 1 alpha-II spectrin alpha-fodrin fodrin alpha chain spectrin, non-erythroid alpha chain spectrin, non-erythroid alpha subunit
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 9 Map Location: 9q34.11
Summary	Spectrins are a family of filamentous cytoskeletal proteins that function as essential scaffold proteins that stabilize the plasma membrane and organize intracellular organelles. Spectrins are composed of alpha and beta dimers that associate to form tetramers linked in a head-to-head arrangement. This gene encodes an alpha spectrin that is specifically expressed in nonerythrocytic cells. The encoded protein has been implicated in other cellular functions including DNA repair and cell cycle regulation. Mutations in this gene are the cause of early infantile epileptic encephalopathy-5. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Sep 2010].
Disorder MIM:	182810
Disorder Html:	Epileptic encephalopathy, early infantile, 5, 613477 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006717252.2	XP_006717315.1	spectrin alpha chain, non-erythrocytic 1 isoform X10
NM_001363759.2	NP_001350688.1	spectrin alpha chain, non-erythrocytic 1 isoform 4
NM_001375310.1	NP_001362239.1	spectrin alpha chain, non-erythrocytic 1 isoform 6
NM_001375313.1	NP_001362242.1	spectrin alpha chain, non-erythrocytic 1 isoform 7
XM_006717248.2	XP_006717311.1	spectrin alpha chain, non-erythrocytic 1 isoform X5
NM_001130438.2	NP_001123910.1	spectrin alpha chain, non-erythrocytic 1 isoform 1
XM_006717251.2	XP_006717314.1	spectrin alpha chain, non-erythrocytic 1 isoform X8
XM_006717252.3	XP_006717315.1	spectrin alpha chain, non-erythrocytic 1 isoform X10
NM_001363765.2	NP_001350694.1	spectrin alpha chain, non-erythrocytic 1 isoform 5
NM_001375314.2	NP_001362243.1	spectrin alpha chain, non-erythrocytic 1 isoform 5
NM_001375312.2	NP_001362241.2	spectrin alpha chain, non-erythrocytic 1 isoform 8
NM_001375311.2	NP_001362240.1	spectrin alpha chain, non-erythrocytic 1 isoform 1
NM_001375318.1	NP_001362247.1	spectrin alpha chain, non-erythrocytic 1 isoform 9
NM_001130438.3	NP_001123910.1	spectrin alpha chain, non-erythrocytic 1 isoform 1
XM_017015061.1	XP_016870550.1	spectrin alpha chain, non-erythrocytic 1 isoform X13
XM_006717253.1	XP_006717316.1	spectrin alpha chain, non-erythrocytic 1 isoform X11
NM_003127.4	NP_003118.2	spectrin alpha chain, non-erythrocytic 1 isoform 2
XM_006717254.2	XP_006717317.1	spectrin alpha chain, non-erythrocytic 1 isoform X3
XM_006717245.1	XP_006717308.1	spectrin alpha chain, non-erythrocytic 1 isoform X1
XM_006717247.2	XP_006717310.1	spectrin alpha chain, non-erythrocytic 1 isoform X4
XM_006717250.1	XP_006717313.1	spectrin alpha chain, non-erythrocytic 1 isoform X7
XM_017015060.1	XP_016870549.1	spectrin alpha chain, non-erythrocytic 1 isoform X12
NM_001363765.1	NP_001350694.1	spectrin alpha chain, non-erythrocytic 1 isoform 5
XM_017015059.1	XP_016870548.1	spectrin alpha chain, non-erythrocytic 1 isoform X9
NM_001195532.1	NP_001182461.1	spectrin alpha chain, non-erythrocytic 1 isoform 3
NM_003127.3	NP_003118.2	spectrin alpha chain, non-erythrocytic 1 isoform 2
NM_001363759.1	NP_001350688.1	spectrin alpha chain, non-erythrocytic 1 isoform 4
XM_006717246.1	XP_006717309.1	spectrin alpha chain, non-erythrocytic 1 isoform X2
XM_006717249.1	XP_006717312.1	spectrin alpha chain, non-erythrocytic 1 isoform X6
NM_001195532.2	NP_001182461.1	spectrin alpha chain, non-erythrocytic 1 isoform 3

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Sptan1	NP_741984.2
Danio rerio (zebrafish)	spna2	NP_001091958.1
Caenorhabditis elegans (roundworm)	spc-1	NP_001257000.1
Xenopus tropicalis (tropical clawed frog)	sptan1	NP_001090674.1
Canis lupus familiaris (dog)	SPTAN1	XP_005625341.1
Bos taurus (cattle)	SPTAN1	NP_001107628.1
Mus musculus (house mouse)	Sptan1	NP_001171138.1
Gallus gallus (chicken)	SPTAN1	NP_001036003.1
Homo sapiens (human)	SPTAN1	NP_001123910.1
	SPTAN1	XP_002800011.1
Drosophila melanogaster (fruit fly)	alpha-Spec	NP_001261286.1
Pan troglodytes (chimpanzee)	SPTAN1	XP_003312345.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006686	XP_316724.1

Gene Ontology
Bibliography

Related articles in PubMed

Critical roles of ?II spectrin in brain development and epileptic encephalopathy.
Wang Y, Ji T, Nelson AD, Glanowska K, Murphy GG, Jenkins PM, Parent JM
The Journal of clinical investigation(2018 Jan)

?II-spectrin in T cells is involved in the regulation of cell-cell contact leading to immunological synapse formation?
Meissner JM, Sikorski AF, Nawara T, Grzesiak J, Marycz K, Bogus?awska DM, Michalczyk I, Lecomte MC, Machnicka B
PloS one12(12)e0189545(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

SPTAN1-related disorders comprise a wide spectrum of neurodevelopmental phenotypes ranging from mild to severe and progressive. Spectrin aggregate formation in fibroblasts with mutations in the alpha/beta heterodimerization domain seems to be associated with a severe neurodegenerative course.
Title: Delineating SPTAN1 associated phenotypes: from isolated epilepsy to encephalopathy with progressive brain atrophy.

Spectrin may be engaged in regulation of distinct events necessary for the establishment and maturity of the immunological synapse.
Title: ?II-spectrin in T cells is involved in the regulation of cell-cell contact leading to immunological synapse formation?

Alpha-spectrin is critical for recruitment of non-ubiquitinated FANCD2 to sites of damage, which has an important role in the repair response and interstrand cross-link repair.
Title: Nuclear ? Spectrin Differentially Affects Monoubiquitinated Versus Non-Ubiquitinated FANCD2 Function After DNA Interstrand Cross-Link Damage.

Studies demonstrate that alpha-IISp plays a critical role in maintaining chromosome stability in cells after DNA interstrand cross-links damage by repairing damage that occurs in both genomic and telomeric DNA.
Title: Functional Significance of Nuclear ? Spectrin.

aggressiveness of MLH1-positive colorectal cancers might be related to SPTAN1.
Title: Reduced migration of MLH1 deficient colon cancer cells depends on SPTAN1.

The following SPTAN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPTAN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu108371	NM_001195532.2 Latest version!	Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OHu108374	NM_003127.4 Latest version!	Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OHu108711	NM_001375311.2 Latest version!	Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OHu108711	NM_001130438.3 Latest version!	Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OHu109488	NM_001375310.1 Latest version!	Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OHu43453	NM_001375318.1 Latest version!	Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant 9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OHu43456	NM_001375312.2 Latest version!	Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OHu98855	NM_001375313.1 Latest version!	Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant 11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OHu98857	NM_001363765.2 Latest version!	Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OHu98857	NM_001375314.2 Latest version!	Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant 10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OHu98855	XM_017015059.1 Latest version!	Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OHu98856	XM_017015060.1 Latest version!	Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OHu98857	XM_017015061.1 Latest version!	Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OHu10360	NM_001195532.1 Latest version!	Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu12626	NM_003127.3 Latest version!	Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu22919	NM_001130438.2 Latest version!	Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu43462	NM_001363759.2 Latest version!	Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OHu43453	XM_006717245.1 Latest version!	Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OHu43454	XM_006717246.1 Latest version!	Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OHu43455	XM_006717247.2 Latest version!	Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OHu43456	XM_006717248.2 Latest version!	Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OHu43457	XM_006717249.1 Latest version!	Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OHu43458	XM_006717250.1 Latest version!	Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OHu43459	XM_006717251.2 Latest version!	Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OHu43460	XM_006717252.3 Latest version!	Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OHu43461	XM_006717253.1 Latest version!	Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OHu43462	XM_006717254.2 Latest version!	Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
View Old Accession Versions >>
OHu43460	XM_006717252.2	Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OHu98857	NM_001363765.1	Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OHu43462	NM_001363759.1	Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPTAN1 ( NM_001195532.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001182461.1 Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu108371
Clone ID Related Accession (Same CDS sequence)	NM_001195532.2
Accession Version	NM_001195532.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	spectrin alpha chain, non-erythrocytic 1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL356481.16. On Jun 2, 2019 this sequence version replaced NM_001195532.1. Transcript Variant: This variant (3) lacks two in-frame exons in the coding region, compared to variant 1. The encoded isoform (3) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: GQ891543.1, BC053521.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACCCAA GTGGGGTCAA AGTGCTGGAA ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTAG ACCGATACCA CCGCTTCAAG GAACTCTCAA CCCTTAGGCG TCAGAAGCTG 
GAAGATTCCT ATCGATTCCA GTTCTTTCAA AGAGATGCTG AAGAGCTGGA GAAATGGATA 
CAGGAAAAAC TTCAGATTGC ATCTGATGAG AATTATAAAG ACCCAACCAA CTTGCAGGGA 
AAGCTTCAGA AGCATCAAGC ATTTGAAGCT GAAGTGCAGG CCAACTCAGG AGCCATTGTT 
AAGCTGGATG AAACTGGAAA CCTGATGATC TCAGAAGGGC ATTTTGCATC TGAAACCATA 
CGGACCCGTT TGATGGAGCT GCACCGCCAG TGGGAATTAC TTTTGGAGAA GATGCGAGAA 
AAAGGAATCA AACTGCTGCA GGCCCAGAAG TTGGTGCAGT ACTTACGAGA ATGTGAGGAC 
GTGATGGACT GGATCAATGA CAAGGAAGCA ATTGTTACTT CTGAAGAGCT GGGCCAGGAT 
CTGGAGCATG TAGAGGTTTT ACAGAAGAAA TTTGAAGAGT TTCAAACAGA TATGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTGAACCAG TTTGCTGCCA AACTCATACA GGAGCAGCAC 
CCTGAGGAGG AACTGATCAA GACTAAGCAG GATGAAGTCA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CTCTGCAGAG GCAGGGGAAG CTCTTTGGGG CAGCAGAAGT TCAGCGCTTT 
AACAGGGATG TGGATGAGAC TATCAGTTGG ATTAAGGAAA AGGAGCAGTT AATGGCCTCT 
GATGATTTTG GCCGAGACCT GGCAAGTGTT CAGGCTCTGC TTCGGAAGCA CGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAAGACAAG GTCAAAGCCC TGTGTGCTGA GGCTGACCGC 
CTGCAACAGT CCCACCCTCT GAGTGCAACA CAGATTCAAG TGAAGCGAGA GGAACTGATT 
ACAAACTGGG AGCAGATCCG CACCTTGGCG GCAGAGAGAC ATGCACGGCT CAATGATTCA 
TACAGGCTTC AACGCTTCCT TGCTGACTTC CGTGACCTCA CCAGCTGGGT GACTGAGATG 
AAAGCCCTCA TCAATGCAGA TGAGCTTGCC AGTGATGTGG CTGGGGCTGA AGCCCTGCTA 
GATAGACACC AAGAGCACAA GGGTGAAATT GATGCCCATG AAGACAGCTT CAAATCTGCA 
GATGAATCTG GACAGGCACT GCTTGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGACCG TCCTTTCCGA GGAGAGAGCG GCGCTGCTGG AGCTGTGGGA GCTGCGCAGG 
CAGCAGTACG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GGGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAATGAAG ACTTGGGAGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTTAA GAAGCACGAA GACTTTGAGA AATCCCTTAG TGCCCAGGAG 
GAAAAGATTA CAGCATTAGA TGAATTTGCA ACCAAGCTAA TTCAGAACAA CCACTATGCA 
ATGGAAGATG TGGCCACTCG CCGAGATGCT CTGTTGAGCC GCCGCAATGC CCTTCACGAG 
AGAGCCATGC GTCGCCGGGC CCAGCTAGCC GATTCTTTCC ATCTGCAGCA GTTTTTCCGT 
GATTCTGATG AGCTCAAGAG TTGGGTCAAT GAGAAGATGA AAACTGCCAC AGATGAAGCT 
TATAAAGATC CATCCAACCT ACAAGGAAAA GTACAGAAGC ATCAGGCTTT TGAGGCTGAG 
CTCTCAGCAA ACCAGAGCCG AATTGATGCC TTGGAGAAAG CTGGCCAAAA GCTGATTGAT 
GTCAACCACT ATGCCAAGGA TGAAGTGGCA GCTCGTATGA ATGAGGTGAT CAGTTTGTGG 
AAGAAACTGC TAGAGGCCAC TGAACTGAAA GGAATAAAGC TTCGTGAAGC CAACCAGCAA 
CAGCAATTTA ATCGCAATGT TGAGGATATT GAATTGTGGC TATATGAAGT AGAAGGTCAC 
TTGGCTTCGG ATGATTACGG CAAAGATCTT ACCAATGTGC AGAACCTCCA GAAGAAACAT 
GCACTGCTAG AGGCAGATGT GGCTGCTCAC CAGGACCGAA TTGATGGCAT CACCATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTT GATGCAGAAA ACATCAAGAA GAAACAGGAA 
GCCCTCGTGG CTCGCTATGA GGCACTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCCGATTCTC TGCGGTTGCA GCAGCTCTTC CGGGATGTTG AGGATGAGGA GACGTGGATT 
CGAGAGAAAG AGCCCATTGC CGCATCTACC AACAGAGGTA AGGATTTAAT TGGGGTCCAG 
AATCTGCTAA AGAAACATCA AGCCTTACAA GCAGAAATTG CTGGACATGA ACCACGCATC 
AAAGCAGTTA CACAGAAGGG GAATGCCATG GTGGAGGAAG GCCATTTTGC TGCAGAGGAT 
GTGAAGGCCA AGCTTCACGA GCTGAACCAA AAGTGGGAGG CACTGAAAGC CAAAGCTTCC 
CAGCGTCGGC AGGACCTGGA GGACTCTCTG CAGGCCCAGC AGTACTTTGC TGATGCTAAC 
GAGGCTGAAT CCTGGATGCG GGAGAAGGAA CCCATTGTGG GCAGCACTGA CTATGGCAAG 
GACGAAGACT CTGCTGAGGC TCTACTGAAG AAACACGAAG CTTTGATGTC AGATCTCAGT 
GCCTACGGCA GCAGCATCCA GGCTTTGCGA GAACAAGCAC AGTCCTGCCG GCAACAAGTG 
GCCCCCACGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CTCTCTACGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAG GGAGATATCC TTACCTTACT CAACAGCACC 
AACAAGGATT GGTGGAAAGT GGAAGTGAAC GATCGTCAGG GTTTTGTGCC GGCTGCGTAC 
GTGAAGAAAT TGGACCCCGC CCAGTCAGCC TCCCGGGAGA ATCTCCTGGA GGAGCAAGGC 
AGCATAGCAC TGCGGCAGGA GCAGATTGAC AATCAATATC ATTCTCTGCT GGAACTGGGT 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TTATGTTGTT CCGTGAAGCG 
AATGAACTAC AGCAATGGAT CAATGAGAAG GAAGCCGCTC TGACAAGTGA GGAGGTCGGA 
GCAGACTTGG AGCAGGTTGA GGTGCTCCAG AAGAAGTTTG ATGACTTCCA GAAGGACCTG 
AAGGCCAATG AGTCACGGTT GAAGGACATT AACAAGGTAG CTGAAGACCT GGAGTCTGAA 
GGTCTCATGG CAGAGGAGGT GCAGGCTGTG CAACAACAGG AAGTGTATGG CATGATGCCC 
AGGGATGAAA CTGATTCCAA GACAGCCTCC CCGTGGAAGT CTGCTCGTCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTACAGCAG 
CTGGCCGAGG AACGGAGCCA GCTCTTGGGC AGCGCCCATG AAGTACAGAG GTTCCACAGA 
GATGCTGATG AAACCAAAGA ATGGATTGAA GAGAAGAATC AAGCTCTAAA CACAGACAAT 
TATGGACATG ATCTCGCCAG TGTCCAGGCC CTGCAACGCA AGCATGAGGG CTTCGAGAGG 
GACCTTGCGG CTCTCGGTGA CAAGGTAAAC TCCCTTGGTG AAACAGCAGA GCGCCTGATC 
CAGTCCCATC CCGAGTCAGC AGAAGACCTG CAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGAGCAGCC TGGGGAAACG TGCAGATCAG CGCAAGGCAA AGTTGGGTGA CTCCCACGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGGGGG 
TTGGTGTCCT CAGATGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCATT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATCGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGACAGC AGCTGTTGGC TCACGGACAC TATGCCAGCC CTGAGATCAA GCAGAAACTT 
GATATTCTTG ACCAGGAGCG TGCAGACCTG GAGAAGGCCT GGGTTCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAACT GCAGCTGTTC CATCGGGACT GTGAGCAAGC TGAGAACTGG 
ATGGCTGCCC GGGAGGCCTT CTTGAATACC GAAGACAAAG GAGACTCACT GGACAGCGTA 
GAGGCTCTGA TCAAAAAACA TGAAGACTTT GACAAAGCGA TTAACGTCCA GGAAGAGAAG 
ATTGCTGCTC TGCAGGCCTT TGCCGACCAG CTCATCGCTG CCGGCCATTA TGCCAAGGGA 
GACATTTCTA GCCGGCGCAA TGAGGTCTTG GACAGGTGGC GACGTCTGAA AGCCCAGATG 
ATTGAGAAAA GGTCAAAGCT AGGAGAATCT CAAACCCTCC AACAGTTCAG CCGGGATGTG 
GATGAGATTG AGGCTTGGAT CAGTGAAAAA TTGCAAACAG CGAGTGATGA GTCGTACAAG 
GATCCCACCA ACATCCAGAG CAAGCACCAG AAGCACCAGG CTTTTGAAGC AGAGCTGCAT 
GCCAACGCTG ACCGGATCCG TGGGGTTATC GACATGGGCA ACTCCCTCAT TGAACGTGGA 
GCCTGTGCCG GCAGTGAGGA TGCTGTCAAG GCCCGCCTGG CTGCCTTAGC TGACCAGTGG 
CAGTTCTTGG TGCAAAAGTC AGCGGAAAAG AGCCAGAAAC TGAAAGAAGC CAACAAGCAG 
CAGAACTTCA ACACAGGGAT CAAGGACTTT GACTTCTGGC TGTCTGAGGT GGAGGCCCTG 
CTGGCATCCG AAGATTATGG CAAAGACCTA GCTTCTGTGA ACAACCTGCT GAAAAAGCAT 
CAACTGCTGG AAGCAGATAT ATCTGCCCAT GAGGATCGCC TGAAGGACCT GAACAGCCAG 
GCAGACAGCC TGATGACCAG CAGTGCCTTC GACACCTCCC AAGTAAAGGA CAAGAGGGAC 
ACCATCAACG GGCGCTTCCA GAAGATCAAG AGCATGGCGG CCTCCCGGCG AGCCAAGCTG 
AATGAATCCC ATCGCCTGCA CCAGTTCTTC CGGGACATGG ATGACGAGGA GTCCTGGATC 
AAGGAGAAGA AGCTGCTGGT GGGCTCAGAG GACTACGGCC GGGACCTAAC CGGCGTGCAG 
AACCTGAGGA AGAAGCACAA GCGGCTGGAA GCAGAACTGG CTGCGCATGA GCCGGCTATT 
CAGGGTGTCC TGGACACTGG CAAGAAGCTG TCCGATGACA ACACCATCGG GAAAGAGGAG 
ATCCAGCAGC GGCTGGCGCA GTTTGTGGAG CACTGGAAAG AGCTGAAGCA GCTGGCAGCT 
GCCCGGGGTC AGCGGCTGGA AGAGTCCTTG GAATATCAGC AGTTTGTAGC CAATGTGGAA 
GAGGAAGAAG CCTGGATCAA TGAGAAAATG ACCCTGGTGG CCAGCGAAGA TTATGGCGAC 
ACTCTTGCCG CCATCCAGGG CTTACTGAAG AAACATGAAG CTTTTGAGAC AGACTTCACC 
GTCCACAAGG ATCGCGTGAA TGATGTCTGC ACCAATGGAC AAGACCTCAT TAAGAAGAAC 
AATCACCATG AGGAGAACAT CTCTTCAAAG ATGAAGGGCC TGAACGGGAA AGTGTCAGAC 
CTGGAGAAAG CTGCAGCCCA GAGAAAGGCG AAGCTGGATG AGAACTCGGC CTTCCTTCAG 
TTCAACTGGA AGGCGGACGT GGTGGAGTCC TGGATCGGTG AAAAGGAGAA CAGCTTGAAG 
ACAGATGATT ATGGCCGAGA CCTGTCTTCT GTGCAGACGC TCCTCACCAA ACAGGAAACT 
TTTGACGCTG GGCTGCAGGC CTTCCAGCAG GAAGGCATTG CCAACATCAC TGCCCTCAAA 
GATCAGCTTC TCGCCGCCAA ACACGTTCAG TCCAAGGCCA TCGAGGCCCG GCACGCCTCC 
CTCATGAAGA GGTGGAGCCA GCTTCTGGCC AACTCAGCCG CCCGCAAGAA GAAGCTTCTG 
GAGGCTCAGA GTCACTTCCG CAAGGTGGAG GACCTCTTCC TGACCTTCGC CAAAAAGGCT 
TCTGCCTTCA ACAGCTGGTT TGAAAATGCA GAGGAGGACT TAACAGACCC CGTGCGCTGC 
AACTCCTTGG AAGAAATCAA AGCTTTGCGC GAGGCCCACG ACGCCTTCCG CTCCTCCCTC 
AGCTCTGCCC AGGCTGACTT CAACCAGCTG GCCGAGCTGG ACCGCCAGAT CAAGAGCTTC 
CGCGTAGCCT CCAACCCCTA CACCTGGTTT ACCATGGAGG CCCTGGAGGA GACCTGGAGG 
AACCTACAGA AAATCATCAA GGAGAGGGAG CTGGAGCTGC AGAAGGAACA GCGGCGGCAG 
GAGGAGAACG ACAAGCTGCG CCAGGAGTTT GCCCAGCACG CCAACGCCTT CCACCAGTGG 
ATCCAAGAGA CCAGGACATA CCTCCTCGAT GGGTCCTGTA TGGTGGAAGA GTCGGGGACC 
CTCGAATCCC AGCTTGAAGC TACCAAACGC AAGCACCAGG AAATCCGAGC CATGAGAAGT 
CAGCTCAAAA AGATCGAGGA CCTGGGGGCC GCCATGGAGG AGGCCCTCAT CCTGGACAAC 
AAGTACACGG AGCACAGCAC CGTGGGCCTC GCCCAGCAGT GGGACCAGCT GGACCAGCTG 
GGCATGCGCA TGCAGCACAA CCTGGAGCAG CAGATCCAGG CCAGGAACAC AACAGGTGTG 
ACTGAGGAGG CCCTCAAAGA ATTCAGCATG ATGTTTAAAC ACTTTGACAA GGACAAGTCT 
GGCAGGCTGA ACCATCAGGA GTTCAAATCT TGCCTGCGCT CCCTGGGCTA TGACCTGCCC 
ATGGTGGAGG AAGGGGAACC TGACCCTGAG TTCGAGGCAA TCCTGGACAC GGTGGATCCG 
AACAGAGATG GCCATGTCTC CTTGCAAGAA TACATGGCTT TCATGATCAG CCGCGAAACT 
GAGAACGTCA AGTCCAGCGA GGAGATTGAG AGCGCCTTCC GGGCCCTCAG CTCAGAGGGA 
AAGCCTTACG TGACCAAGGA GGAGCTCTAC CAGAACCTGA CCCGGGAACA AGCCGACTAC 
TGCGTCTCCC ACATGAAGCC CTACGTGGAC GGCAAGGGCC GCGAGCTCCC CACCGCGTTC 
GACTACGTGG AGTTCACCCG CTCGCTTTTC GTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001182461.1
CDS	114..7472
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELASDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMRRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPTDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGMMPRDETDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKQKLDILDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAAGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINGRFQKIKSMAASRRAKLNESHRLHQFFRDMDDEESWIKEKKLLVGSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHASLMKRWSQLLANSAARKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	NM_001195532.2
Organism	Homo sapiens(human)
Definition	Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001195532.2

ORF Insert Sequence:

ATGGACCCAA GTGGGGTCAA AGTGCTGGAA ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTAG ACCGATACCA CCGCTTCAAG GAACTCTCAA CCCTTAGGCG TCAGAAGCTG 
GAAGATTCCT ATCGATTCCA GTTCTTTCAA AGAGATGCTG AAGAGCTGGA GAAATGGATA 
CAGGAAAAAC TTCAGATTGC ATCTGATGAG AATTATAAAG ACCCAACCAA CTTGCAGGGA 
AAGCTTCAGA AGCATCAAGC ATTTGAAGCT GAAGTGCAGG CCAACTCAGG AGCCATTGTT 
AAGCTGGATG AAACTGGAAA CCTGATGATC TCAGAAGGGC ATTTTGCATC TGAAACCATA 
CGGACCCGTT TGATGGAGCT GCACCGCCAG TGGGAATTAC TTTTGGAGAA GATGCGAGAA 
AAAGGAATCA AACTGCTGCA GGCCCAGAAG TTGGTGCAGT ACTTACGAGA ATGTGAGGAC 
GTGATGGACT GGATCAATGA CAAGGAAGCA ATTGTTACTT CTGAAGAGCT GGGCCAGGAT 
CTGGAGCATG TAGAGGTTTT ACAGAAGAAA TTTGAAGAGT TTCAAACAGA TATGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTGAACCAG TTTGCTGCCA AACTCATACA GGAGCAGCAC 
CCTGAGGAGG AACTGATCAA GACTAAGCAG GATGAAGTCA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CTCTGCAGAG GCAGGGGAAG CTCTTTGGGG CAGCAGAAGT TCAGCGCTTT 
AACAGGGATG TGGATGAGAC TATCAGTTGG ATTAAGGAAA AGGAGCAGTT AATGGCCTCT 
GATGATTTTG GCCGAGACCT GGCAAGTGTT CAGGCTCTGC TTCGGAAGCA CGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAAGACAAG GTCAAAGCCC TGTGTGCTGA GGCTGACCGC 
CTGCAACAGT CCCACCCTCT GAGTGCAACA CAGATTCAAG TGAAGCGAGA GGAACTGATT 
ACAAACTGGG AGCAGATCCG CACCTTGGCG GCAGAGAGAC ATGCACGGCT CAATGATTCA 
TACAGGCTTC AACGCTTCCT TGCTGACTTC CGTGACCTCA CCAGCTGGGT GACTGAGATG 
AAAGCCCTCA TCAATGCAGA TGAGCTTGCC AGTGATGTGG CTGGGGCTGA AGCCCTGCTA 
GATAGACACC AAGAGCACAA GGGTGAAATT GATGCCCATG AAGACAGCTT CAAATCTGCA 
GATGAATCTG GACAGGCACT GCTTGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGACCG TCCTTTCCGA GGAGAGAGCG GCGCTGCTGG AGCTGTGGGA GCTGCGCAGG 
CAGCAGTACG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GGGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAATGAAG ACTTGGGAGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTTAA GAAGCACGAA GACTTTGAGA AATCCCTTAG TGCCCAGGAG 
GAAAAGATTA CAGCATTAGA TGAATTTGCA ACCAAGCTAA TTCAGAACAA CCACTATGCA 
ATGGAAGATG TGGCCACTCG CCGAGATGCT CTGTTGAGCC GCCGCAATGC CCTTCACGAG 
AGAGCCATGC GTCGCCGGGC CCAGCTAGCC GATTCTTTCC ATCTGCAGCA GTTTTTCCGT 
GATTCTGATG AGCTCAAGAG TTGGGTCAAT GAGAAGATGA AAACTGCCAC AGATGAAGCT 
TATAAAGATC CATCCAACCT ACAAGGAAAA GTACAGAAGC ATCAGGCTTT TGAGGCTGAG 
CTCTCAGCAA ACCAGAGCCG AATTGATGCC TTGGAGAAAG CTGGCCAAAA GCTGATTGAT 
GTCAACCACT ATGCCAAGGA TGAAGTGGCA GCTCGTATGA ATGAGGTGAT CAGTTTGTGG 
AAGAAACTGC TAGAGGCCAC TGAACTGAAA GGAATAAAGC TTCGTGAAGC CAACCAGCAA 
CAGCAATTTA ATCGCAATGT TGAGGATATT GAATTGTGGC TATATGAAGT AGAAGGTCAC 
TTGGCTTCGG ATGATTACGG CAAAGATCTT ACCAATGTGC AGAACCTCCA GAAGAAACAT 
GCACTGCTAG AGGCAGATGT GGCTGCTCAC CAGGACCGAA TTGATGGCAT CACCATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTT GATGCAGAAA ACATCAAGAA GAAACAGGAA 
GCCCTCGTGG CTCGCTATGA GGCACTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCCGATTCTC TGCGGTTGCA GCAGCTCTTC CGGGATGTTG AGGATGAGGA GACGTGGATT 
CGAGAGAAAG AGCCCATTGC CGCATCTACC AACAGAGGTA AGGATTTAAT TGGGGTCCAG 
AATCTGCTAA AGAAACATCA AGCCTTACAA GCAGAAATTG CTGGACATGA ACCACGCATC 
AAAGCAGTTA CACAGAAGGG GAATGCCATG GTGGAGGAAG GCCATTTTGC TGCAGAGGAT 
GTGAAGGCCA AGCTTCACGA GCTGAACCAA AAGTGGGAGG CACTGAAAGC CAAAGCTTCC 
CAGCGTCGGC AGGACCTGGA GGACTCTCTG CAGGCCCAGC AGTACTTTGC TGATGCTAAC 
GAGGCTGAAT CCTGGATGCG GGAGAAGGAA CCCATTGTGG GCAGCACTGA CTATGGCAAG 
GACGAAGACT CTGCTGAGGC TCTACTGAAG AAACACGAAG CTTTGATGTC AGATCTCAGT 
GCCTACGGCA GCAGCATCCA GGCTTTGCGA GAACAAGCAC AGTCCTGCCG GCAACAAGTG 
GCCCCCACGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CTCTCTACGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAG GGAGATATCC TTACCTTACT CAACAGCACC 
AACAAGGATT GGTGGAAAGT GGAAGTGAAC GATCGTCAGG GTTTTGTGCC GGCTGCGTAC 
GTGAAGAAAT TGGACCCCGC CCAGTCAGCC TCCCGGGAGA ATCTCCTGGA GGAGCAAGGC 
AGCATAGCAC TGCGGCAGGA GCAGATTGAC AATCAATATC ATTCTCTGCT GGAACTGGGT 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TTATGTTGTT CCGTGAAGCG 
AATGAACTAC AGCAATGGAT CAATGAGAAG GAAGCCGCTC TGACAAGTGA GGAGGTCGGA 
GCAGACTTGG AGCAGGTTGA GGTGCTCCAG AAGAAGTTTG ATGACTTCCA GAAGGACCTG 
AAGGCCAATG AGTCACGGTT GAAGGACATT AACAAGGTAG CTGAAGACCT GGAGTCTGAA 
GGTCTCATGG CAGAGGAGGT GCAGGCTGTG CAACAACAGG AAGTGTATGG CATGATGCCC 
AGGGATGAAA CTGATTCCAA GACAGCCTCC CCGTGGAAGT CTGCTCGTCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTACAGCAG 
CTGGCCGAGG AACGGAGCCA GCTCTTGGGC AGCGCCCATG AAGTACAGAG GTTCCACAGA 
GATGCTGATG AAACCAAAGA ATGGATTGAA GAGAAGAATC AAGCTCTAAA CACAGACAAT 
TATGGACATG ATCTCGCCAG TGTCCAGGCC CTGCAACGCA AGCATGAGGG CTTCGAGAGG 
GACCTTGCGG CTCTCGGTGA CAAGGTAAAC TCCCTTGGTG AAACAGCAGA GCGCCTGATC 
CAGTCCCATC CCGAGTCAGC AGAAGACCTG CAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGAGCAGCC TGGGGAAACG TGCAGATCAG CGCAAGGCAA AGTTGGGTGA CTCCCACGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGGGGG 
TTGGTGTCCT CAGATGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCATT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATCGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGACAGC AGCTGTTGGC TCACGGACAC TATGCCAGCC CTGAGATCAA GCAGAAACTT 
GATATTCTTG ACCAGGAGCG TGCAGACCTG GAGAAGGCCT GGGTTCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAACT GCAGCTGTTC CATCGGGACT GTGAGCAAGC TGAGAACTGG 
ATGGCTGCCC GGGAGGCCTT CTTGAATACC GAAGACAAAG GAGACTCACT GGACAGCGTA 
GAGGCTCTGA TCAAAAAACA TGAAGACTTT GACAAAGCGA TTAACGTCCA GGAAGAGAAG 
ATTGCTGCTC TGCAGGCCTT TGCCGACCAG CTCATCGCTG CCGGCCATTA TGCCAAGGGA 
GACATTTCTA GCCGGCGCAA TGAGGTCTTG GACAGGTGGC GACGTCTGAA AGCCCAGATG 
ATTGAGAAAA GGTCAAAGCT AGGAGAATCT CAAACCCTCC AACAGTTCAG CCGGGATGTG 
GATGAGATTG AGGCTTGGAT CAGTGAAAAA TTGCAAACAG CGAGTGATGA GTCGTACAAG 
GATCCCACCA ACATCCAGAG CAAGCACCAG AAGCACCAGG CTTTTGAAGC AGAGCTGCAT 
GCCAACGCTG ACCGGATCCG TGGGGTTATC GACATGGGCA ACTCCCTCAT TGAACGTGGA 
GCCTGTGCCG GCAGTGAGGA TGCTGTCAAG GCCCGCCTGG CTGCCTTAGC TGACCAGTGG 
CAGTTCTTGG TGCAAAAGTC AGCGGAAAAG AGCCAGAAAC TGAAAGAAGC CAACAAGCAG 
CAGAACTTCA ACACAGGGAT CAAGGACTTT GACTTCTGGC TGTCTGAGGT GGAGGCCCTG 
CTGGCATCCG AAGATTATGG CAAAGACCTA GCTTCTGTGA ACAACCTGCT GAAAAAGCAT 
CAACTGCTGG AAGCAGATAT ATCTGCCCAT GAGGATCGCC TGAAGGACCT GAACAGCCAG 
GCAGACAGCC TGATGACCAG CAGTGCCTTC GACACCTCCC AAGTAAAGGA CAAGAGGGAC 
ACCATCAACG GGCGCTTCCA GAAGATCAAG AGCATGGCGG CCTCCCGGCG AGCCAAGCTG 
AATGAATCCC ATCGCCTGCA CCAGTTCTTC CGGGACATGG ATGACGAGGA GTCCTGGATC 
AAGGAGAAGA AGCTGCTGGT GGGCTCAGAG GACTACGGCC GGGACCTAAC CGGCGTGCAG 
AACCTGAGGA AGAAGCACAA GCGGCTGGAA GCAGAACTGG CTGCGCATGA GCCGGCTATT 
CAGGGTGTCC TGGACACTGG CAAGAAGCTG TCCGATGACA ACACCATCGG GAAAGAGGAG 
ATCCAGCAGC GGCTGGCGCA GTTTGTGGAG CACTGGAAAG AGCTGAAGCA GCTGGCAGCT 
GCCCGGGGTC AGCGGCTGGA AGAGTCCTTG GAATATCAGC AGTTTGTAGC CAATGTGGAA 
GAGGAAGAAG CCTGGATCAA TGAGAAAATG ACCCTGGTGG CCAGCGAAGA TTATGGCGAC 
ACTCTTGCCG CCATCCAGGG CTTACTGAAG AAACATGAAG CTTTTGAGAC AGACTTCACC 
GTCCACAAGG ATCGCGTGAA TGATGTCTGC ACCAATGGAC AAGACCTCAT TAAGAAGAAC 
AATCACCATG AGGAGAACAT CTCTTCAAAG ATGAAGGGCC TGAACGGGAA AGTGTCAGAC 
CTGGAGAAAG CTGCAGCCCA GAGAAAGGCG AAGCTGGATG AGAACTCGGC CTTCCTTCAG 
TTCAACTGGA AGGCGGACGT GGTGGAGTCC TGGATCGGTG AAAAGGAGAA CAGCTTGAAG 
ACAGATGATT ATGGCCGAGA CCTGTCTTCT GTGCAGACGC TCCTCACCAA ACAGGAAACT 
TTTGACGCTG GGCTGCAGGC CTTCCAGCAG GAAGGCATTG CCAACATCAC TGCCCTCAAA 
GATCAGCTTC TCGCCGCCAA ACACGTTCAG TCCAAGGCCA TCGAGGCCCG GCACGCCTCC 
CTCATGAAGA GGTGGAGCCA GCTTCTGGCC AACTCAGCCG CCCGCAAGAA GAAGCTTCTG 
GAGGCTCAGA GTCACTTCCG CAAGGTGGAG GACCTCTTCC TGACCTTCGC CAAAAAGGCT 
TCTGCCTTCA ACAGCTGGTT TGAAAATGCA GAGGAGGACT TAACAGACCC CGTGCGCTGC 
AACTCCTTGG AAGAAATCAA AGCTTTGCGC GAGGCCCACG ACGCCTTCCG CTCCTCCCTC 
AGCTCTGCCC AGGCTGACTT CAACCAGCTG GCCGAGCTGG ACCGCCAGAT CAAGAGCTTC 
CGCGTAGCCT CCAACCCCTA CACCTGGTTT ACCATGGAGG CCCTGGAGGA GACCTGGAGG 
AACCTACAGA AAATCATCAA GGAGAGGGAG CTGGAGCTGC AGAAGGAACA GCGGCGGCAG 
GAGGAGAACG ACAAGCTGCG CCAGGAGTTT GCCCAGCACG CCAACGCCTT CCACCAGTGG 
ATCCAAGAGA CCAGGACATA CCTCCTCGAT GGGTCCTGTA TGGTGGAAGA GTCGGGGACC 
CTCGAATCCC AGCTTGAAGC TACCAAACGC AAGCACCAGG AAATCCGAGC CATGAGAAGT 
CAGCTCAAAA AGATCGAGGA CCTGGGGGCC GCCATGGAGG AGGCCCTCAT CCTGGACAAC 
AAGTACACGG AGCACAGCAC CGTGGGCCTC GCCCAGCAGT GGGACCAGCT GGACCAGCTG 
GGCATGCGCA TGCAGCACAA CCTGGAGCAG CAGATCCAGG CCAGGAACAC AACAGGTGTG 
ACTGAGGAGG CCCTCAAAGA ATTCAGCATG ATGTTTAAAC ACTTTGACAA GGACAAGTCT 
GGCAGGCTGA ACCATCAGGA GTTCAAATCT TGCCTGCGCT CCCTGGGCTA TGACCTGCCC 
ATGGTGGAGG AAGGGGAACC TGACCCTGAG TTCGAGGCAA TCCTGGACAC GGTGGATCCG 
AACAGAGATG GCCATGTCTC CTTGCAAGAA TACATGGCTT TCATGATCAG CCGCGAAACT 
GAGAACGTCA AGTCCAGCGA GGAGATTGAG AGCGCCTTCC GGGCCCTCAG CTCAGAGGGA 
AAGCCTTACG TGACCAAGGA GGAGCTCTAC CAGAACCTGA CCCGGGAACA AGCCGACTAC 
TGCGTCTCCC ACATGAAGCC CTACGTGGAC GGCAAGGGCC GCGAGCTCCC CACCGCGTTC 
GACTACGTGG AGTTCACCCG CTCGCTTTTC GTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( NM_003127.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_003118.2 Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant 2, mRNA.

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CloneID	OHu108374
Clone ID Related Accession (Same CDS sequence)	NM_003127.4
Accession Version	NM_003127.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7419bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	spectrin alpha chain, non-erythrocytic 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL356481.16. On Jun 2, 2019 this sequence version replaced NM_003127.3. Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, compared to variant 1. The resulting isoform (2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB209860.1, J05243.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACCCAA GTGGGGTCAA AGTGCTGGAA ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTAG ACCGATACCA CCGCTTCAAG GAACTCTCAA CCCTTAGGCG TCAGAAGCTG 
GAAGATTCCT ATCGATTCCA GTTCTTTCAA AGAGATGCTG AAGAGCTGGA GAAATGGATA 
CAGGAAAAAC TTCAGATTGC ATCTGATGAG AATTATAAAG ACCCAACCAA CTTGCAGGGA 
AAGCTTCAGA AGCATCAAGC ATTTGAAGCT GAAGTGCAGG CCAACTCAGG AGCCATTGTT 
AAGCTGGATG AAACTGGAAA CCTGATGATC TCAGAAGGGC ATTTTGCATC TGAAACCATA 
CGGACCCGTT TGATGGAGCT GCACCGCCAG TGGGAATTAC TTTTGGAGAA GATGCGAGAA 
AAAGGAATCA AACTGCTGCA GGCCCAGAAG TTGGTGCAGT ACTTACGAGA ATGTGAGGAC 
GTGATGGACT GGATCAATGA CAAGGAAGCA ATTGTTACTT CTGAAGAGCT GGGCCAGGAT 
CTGGAGCATG TAGAGGTTTT ACAGAAGAAA TTTGAAGAGT TTCAAACAGA TATGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTGAACCAG TTTGCTGCCA AACTCATACA GGAGCAGCAC 
CCTGAGGAGG AACTGATCAA GACTAAGCAG GATGAAGTCA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CTCTGCAGAG GCAGGGGAAG CTCTTTGGGG CAGCAGAAGT TCAGCGCTTT 
AACAGGGATG TGGATGAGAC TATCAGTTGG ATTAAGGAAA AGGAGCAGTT AATGGCCTCT 
GATGATTTTG GCCGAGACCT GGCAAGTGTT CAGGCTCTGC TTCGGAAGCA CGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAAGACAAG GTCAAAGCCC TGTGTGCTGA GGCTGACCGC 
CTGCAACAGT CCCACCCTCT GAGTGCAACA CAGATTCAAG TGAAGCGAGA GGAACTGATT 
ACAAACTGGG AGCAGATCCG CACCTTGGCG GCAGAGAGAC ATGCACGGCT CAATGATTCA 
TACAGGCTTC AACGCTTCCT TGCTGACTTC CGTGACCTCA CCAGCTGGGT GACTGAGATG 
AAAGCCCTCA TCAATGCAGA TGAGCTTGCC AGTGATGTGG CTGGGGCTGA AGCCCTGCTA 
GATAGACACC AAGAGCACAA GGGTGAAATT GATGCCCATG AAGACAGCTT CAAATCTGCA 
GATGAATCTG GACAGGCACT GCTTGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGACCG TCCTTTCCGA GGAGAGAGCG GCGCTGCTGG AGCTGTGGGA GCTGCGCAGG 
CAGCAGTACG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GGGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAATGAAG ACTTGGGAGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTTAA GAAGCACGAA GACTTTGAGA AATCCCTTAG TGCCCAGGAG 
GAAAAGATTA CAGCATTAGA TGAATTTGCA ACCAAGCTAA TTCAGAACAA CCACTATGCA 
ATGGAAGATG TGGCCACTCG CCGAGATGCT CTGTTGAGCC GCCGCAATGC CCTTCACGAG 
AGAGCCATGC GTCGCCGGGC CCAGCTAGCC GATTCTTTCC ATCTGCAGCA GTTTTTCCGT 
GATTCTGATG AGCTCAAGAG TTGGGTCAAT GAGAAGATGA AAACTGCCAC AGATGAAGCT 
TATAAAGATC CATCCAACCT ACAAGGAAAA GTACAGAAGC ATCAGGCTTT TGAGGCTGAG 
CTCTCAGCAA ACCAGAGCCG AATTGATGCC TTGGAGAAAG CTGGCCAAAA GCTGATTGAT 
GTCAACCACT ATGCCAAGGA TGAAGTGGCA GCTCGTATGA ATGAGGTGAT CAGTTTGTGG 
AAGAAACTGC TAGAGGCCAC TGAACTGAAA GGAATAAAGC TTCGTGAAGC CAACCAGCAA 
CAGCAATTTA ATCGCAATGT TGAGGATATT GAATTGTGGC TATATGAAGT AGAAGGTCAC 
TTGGCTTCGG ATGATTACGG CAAAGATCTT ACCAATGTGC AGAACCTCCA GAAGAAACAT 
GCACTGCTAG AGGCAGATGT GGCTGCTCAC CAGGACCGAA TTGATGGCAT CACCATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTT GATGCAGAAA ACATCAAGAA GAAACAGGAA 
GCCCTCGTGG CTCGCTATGA GGCACTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCCGATTCTC TGCGGTTGCA GCAGCTCTTC CGGGATGTTG AGGATGAGGA GACGTGGATT 
CGAGAGAAAG AGCCCATTGC CGCATCTACC AACAGAGGTA AGGATTTAAT TGGGGTCCAG 
AATCTGCTAA AGAAACATCA AGCCTTACAA GCAGAAATTG CTGGACATGA ACCACGCATC 
AAAGCAGTTA CACAGAAGGG GAATGCCATG GTGGAGGAAG GCCATTTTGC TGCAGAGGAT 
GTGAAGGCCA AGCTTCACGA GCTGAACCAA AAGTGGGAGG CACTGAAAGC CAAAGCTTCC 
CAGCGTCGGC AGGACCTGGA GGACTCTCTG CAGGCCCAGC AGTACTTTGC TGATGCTAAC 
GAGGCTGAAT CCTGGATGCG GGAGAAGGAA CCCATTGTGG GCAGCACTGA CTATGGCAAG 
GACGAAGACT CTGCTGAGGC TCTACTGAAG AAACACGAAG CTTTGATGTC AGATCTCAGT 
GCCTACGGCA GCAGCATCCA GGCTTTGCGA GAACAAGCAC AGTCCTGCCG GCAACAAGTG 
GCCCCCACGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CTCTCTACGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAG GGAGATATCC TTACCTTACT CAACAGCACC 
AACAAGGATT GGTGGAAAGT GGAAGTGAAC GATCGTCAGG GTTTTGTGCC GGCTGCGTAC 
GTGAAGAAAT TGGACCCCGC CCAGTCAGCC TCCCGGGAGA ATCTCCTGGA GGAGCAAGGC 
AGCATAGCAC TGCGGCAGGA GCAGATTGAC AATCAGACAC GCATAACTAA GGAGGCCGGC 
AGTGTATCTC TGCGTATGAA GCAGGTGGAA GAACTATATC ATTCTCTGCT GGAACTGGGT 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TTATGTTGTT CCGTGAAGCG 
AATGAACTAC AGCAATGGAT CAATGAGAAG GAAGCCGCTC TGACAAGTGA GGAGGTCGGA 
GCAGACTTGG AGCAGGTTGA GGTGCTCCAG AAGAAGTTTG ATGACTTCCA GAAGGACCTG 
AAGGCCAATG AGTCACGGTT GAAGGACATT AACAAGGTAG CTGAAGACCT GGAGTCTGAA 
GGTCTCATGG CAGAGGAGGT GCAGGCTGTG CAACAACAGG AAGTGTATGG CATGATGCCC 
AGGGATGAAA CTGATTCCAA GACAGCCTCC CCGTGGAAGT CTGCTCGTCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTACAGCAG 
CTGGCCGAGG AACGGAGCCA GCTCTTGGGC AGCGCCCATG AAGTACAGAG GTTCCACAGA 
GATGCTGATG AAACCAAAGA ATGGATTGAA GAGAAGAATC AAGCTCTAAA CACAGACAAT 
TATGGACATG ATCTCGCCAG TGTCCAGGCC CTGCAACGCA AGCATGAGGG CTTCGAGAGG 
GACCTTGCGG CTCTCGGTGA CAAGGTAAAC TCCCTTGGTG AAACAGCAGA GCGCCTGATC 
CAGTCCCATC CCGAGTCAGC AGAAGACCTG CAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGAGCAGCC TGGGGAAACG TGCAGATCAG CGCAAGGCAA AGTTGGGTGA CTCCCACGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGGGGG 
TTGGTGTCCT CAGATGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCATT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATCGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGACAGC AGCTGTTGGC TCACGGACAC TATGCCAGCC CTGAGATCAA GCAGAAACTT 
GATATTCTTG ACCAGGAGCG TGCAGACCTG GAGAAGGCCT GGGTTCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAACT GCAGCTGTTC CATCGGGACT GTGAGCAAGC TGAGAACTGG 
ATGGCTGCCC GGGAGGCCTT CTTGAATACC GAAGACAAAG GAGACTCACT GGACAGCGTA 
GAGGCTCTGA TCAAAAAACA TGAAGACTTT GACAAAGCGA TTAACGTCCA GGAAGAGAAG 
ATTGCTGCTC TGCAGGCCTT TGCCGACCAG CTCATCGCTG CCGGCCATTA TGCCAAGGGA 
GACATTTCTA GCCGGCGCAA TGAGGTCTTG GACAGGTGGC GACGTCTGAA AGCCCAGATG 
ATTGAGAAAA GGTCAAAGCT AGGAGAATCT CAAACCCTCC AACAGTTCAG CCGGGATGTG 
GATGAGATTG AGGCTTGGAT CAGTGAAAAA TTGCAAACAG CGAGTGATGA GTCGTACAAG 
GATCCCACCA ACATCCAGAG CAAGCACCAG AAGCACCAGG CTTTTGAAGC AGAGCTGCAT 
GCCAACGCTG ACCGGATCCG TGGGGTTATC GACATGGGCA ACTCCCTCAT TGAACGTGGA 
GCCTGTGCCG GCAGTGAGGA TGCTGTCAAG GCCCGCCTGG CTGCCTTAGC TGACCAGTGG 
CAGTTCTTGG TGCAAAAGTC AGCGGAAAAG AGCCAGAAAC TGAAAGAAGC CAACAAGCAG 
CAGAACTTCA ACACAGGGAT CAAGGACTTT GACTTCTGGC TGTCTGAGGT GGAGGCCCTG 
CTGGCATCCG AAGATTATGG CAAAGACCTA GCTTCTGTGA ACAACCTGCT GAAAAAGCAT 
CAACTGCTGG AAGCAGATAT ATCTGCCCAT GAGGATCGCC TGAAGGACCT GAACAGCCAG 
GCAGACAGCC TGATGACCAG CAGTGCCTTC GACACCTCCC AAGTAAAGGA CAAGAGGGAC 
ACCATCAACG GGCGCTTCCA GAAGATCAAG AGCATGGCGG CCTCCCGGCG AGCCAAGCTG 
AATGAATCCC ATCGCCTGCA CCAGTTCTTC CGGGACATGG ATGACGAGGA GTCCTGGATC 
AAGGAGAAGA AGCTGCTGGT GGGCTCAGAG GACTACGGCC GGGACCTAAC CGGCGTGCAG 
AACCTGAGGA AGAAGCACAA GCGGCTGGAA GCAGAACTGG CTGCGCATGA GCCGGCTATT 
CAGGGTGTCC TGGACACTGG CAAGAAGCTG TCCGATGACA ACACCATCGG GAAAGAGGAG 
ATCCAGCAGC GGCTGGCGCA GTTTGTGGAG CACTGGAAAG AGCTGAAGCA GCTGGCAGCT 
GCCCGGGGTC AGCGGCTGGA AGAGTCCTTG GAATATCAGC AGTTTGTAGC CAATGTGGAA 
GAGGAAGAAG CCTGGATCAA TGAGAAAATG ACCCTGGTGG CCAGCGAAGA TTATGGCGAC 
ACTCTTGCCG CCATCCAGGG CTTACTGAAG AAACATGAAG CTTTTGAGAC AGACTTCACC 
GTCCACAAGG ATCGCGTGAA TGATGTCTGC ACCAATGGAC AAGACCTCAT TAAGAAGAAC 
AATCACCATG AGGAGAACAT CTCTTCAAAG ATGAAGGGCC TGAACGGGAA AGTGTCAGAC 
CTGGAGAAAG CTGCAGCCCA GAGAAAGGCG AAGCTGGATG AGAACTCGGC CTTCCTTCAG 
TTCAACTGGA AGGCGGACGT GGTGGAGTCC TGGATCGGTG AAAAGGAGAA CAGCTTGAAG 
ACAGATGATT ATGGCCGAGA CCTGTCTTCT GTGCAGACGC TCCTCACCAA ACAGGAAACT 
TTTGACGCTG GGCTGCAGGC CTTCCAGCAG GAAGGCATTG CCAACATCAC TGCCCTCAAA 
GATCAGCTTC TCGCCGCCAA ACACGTTCAG TCCAAGGCCA TCGAGGCCCG GCACGCCTCC 
CTCATGAAGA GGTGGAGCCA GCTTCTGGCC AACTCAGCCG CCCGCAAGAA GAAGCTTCTG 
GAGGCTCAGA GTCACTTCCG CAAGGTGGAG GACCTCTTCC TGACCTTCGC CAAAAAGGCT 
TCTGCCTTCA ACAGCTGGTT TGAAAATGCA GAGGAGGACT TAACAGACCC CGTGCGCTGC 
AACTCCTTGG AAGAAATCAA AGCTTTGCGC GAGGCCCACG ACGCCTTCCG CTCCTCCCTC 
AGCTCTGCCC AGGCTGACTT CAACCAGCTG GCCGAGCTGG ACCGCCAGAT CAAGAGCTTC 
CGCGTAGCCT CCAACCCCTA CACCTGGTTT ACCATGGAGG CCCTGGAGGA GACCTGGAGG 
AACCTACAGA AAATCATCAA GGAGAGGGAG CTGGAGCTGC AGAAGGAACA GCGGCGGCAG 
GAGGAGAACG ACAAGCTGCG CCAGGAGTTT GCCCAGCACG CCAACGCCTT CCACCAGTGG 
ATCCAAGAGA CCAGGACATA CCTCCTCGAT GGGTCCTGTA TGGTGGAAGA GTCGGGGACC 
CTCGAATCCC AGCTTGAAGC TACCAAACGC AAGCACCAGG AAATCCGAGC CATGAGAAGT 
CAGCTCAAAA AGATCGAGGA CCTGGGGGCC GCCATGGAGG AGGCCCTCAT CCTGGACAAC 
AAGTACACGG AGCACAGCAC CGTGGGCCTC GCCCAGCAGT GGGACCAGCT GGACCAGCTG 
GGCATGCGCA TGCAGCACAA CCTGGAGCAG CAGATCCAGG CCAGGAACAC AACAGGTGTG 
ACTGAGGAGG CCCTCAAAGA ATTCAGCATG ATGTTTAAAC ACTTTGACAA GGACAAGTCT 
GGCAGGCTGA ACCATCAGGA GTTCAAATCT TGCCTGCGCT CCCTGGGCTA TGACCTGCCC 
ATGGTGGAGG AAGGGGAACC TGACCCTGAG TTCGAGGCAA TCCTGGACAC GGTGGATCCG 
AACAGAGATG GCCATGTCTC CTTGCAAGAA TACATGGCTT TCATGATCAG CCGCGAAACT 
GAGAACGTCA AGTCCAGCGA GGAGATTGAG AGCGCCTTCC GGGCCCTCAG CTCAGAGGGA 
AAGCCTTACG TGACCAAGGA GGAGCTCTAC CAGAACCTGA CCCGGGAACA AGCCGACTAC 
TGCGTCTCCC ACATGAAGCC CTACGTGGAC GGCAAGGGCC GCGAGCTCCC CACCGCGTTC 
GACTACGTGG AGTTCACCCG CTCGCTTTTC GTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003118.2
CDS	114..7532
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELASDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMRRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPTDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRITKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGMMPRDETDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKQKLDILDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAAGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINGRFQKIKSMAASRRAKLNESHRLHQFFRDMDDEESWIKEKKLLVGSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHASLMKRWSQLLANSAARKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	NM_003127.4
Organism	Homo sapiens(human)
Definition	Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003127.4

ORF Insert Sequence:

ATGGACCCAA GTGGGGTCAA AGTGCTGGAA ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTAG ACCGATACCA CCGCTTCAAG GAACTCTCAA CCCTTAGGCG TCAGAAGCTG 
GAAGATTCCT ATCGATTCCA GTTCTTTCAA AGAGATGCTG AAGAGCTGGA GAAATGGATA 
CAGGAAAAAC TTCAGATTGC ATCTGATGAG AATTATAAAG ACCCAACCAA CTTGCAGGGA 
AAGCTTCAGA AGCATCAAGC ATTTGAAGCT GAAGTGCAGG CCAACTCAGG AGCCATTGTT 
AAGCTGGATG AAACTGGAAA CCTGATGATC TCAGAAGGGC ATTTTGCATC TGAAACCATA 
CGGACCCGTT TGATGGAGCT GCACCGCCAG TGGGAATTAC TTTTGGAGAA GATGCGAGAA 
AAAGGAATCA AACTGCTGCA GGCCCAGAAG TTGGTGCAGT ACTTACGAGA ATGTGAGGAC 
GTGATGGACT GGATCAATGA CAAGGAAGCA ATTGTTACTT CTGAAGAGCT GGGCCAGGAT 
CTGGAGCATG TAGAGGTTTT ACAGAAGAAA TTTGAAGAGT TTCAAACAGA TATGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTGAACCAG TTTGCTGCCA AACTCATACA GGAGCAGCAC 
CCTGAGGAGG AACTGATCAA GACTAAGCAG GATGAAGTCA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CTCTGCAGAG GCAGGGGAAG CTCTTTGGGG CAGCAGAAGT TCAGCGCTTT 
AACAGGGATG TGGATGAGAC TATCAGTTGG ATTAAGGAAA AGGAGCAGTT AATGGCCTCT 
GATGATTTTG GCCGAGACCT GGCAAGTGTT CAGGCTCTGC TTCGGAAGCA CGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAAGACAAG GTCAAAGCCC TGTGTGCTGA GGCTGACCGC 
CTGCAACAGT CCCACCCTCT GAGTGCAACA CAGATTCAAG TGAAGCGAGA GGAACTGATT 
ACAAACTGGG AGCAGATCCG CACCTTGGCG GCAGAGAGAC ATGCACGGCT CAATGATTCA 
TACAGGCTTC AACGCTTCCT TGCTGACTTC CGTGACCTCA CCAGCTGGGT GACTGAGATG 
AAAGCCCTCA TCAATGCAGA TGAGCTTGCC AGTGATGTGG CTGGGGCTGA AGCCCTGCTA 
GATAGACACC AAGAGCACAA GGGTGAAATT GATGCCCATG AAGACAGCTT CAAATCTGCA 
GATGAATCTG GACAGGCACT GCTTGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGACCG TCCTTTCCGA GGAGAGAGCG GCGCTGCTGG AGCTGTGGGA GCTGCGCAGG 
CAGCAGTACG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GGGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAATGAAG ACTTGGGAGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTTAA GAAGCACGAA GACTTTGAGA AATCCCTTAG TGCCCAGGAG 
GAAAAGATTA CAGCATTAGA TGAATTTGCA ACCAAGCTAA TTCAGAACAA CCACTATGCA 
ATGGAAGATG TGGCCACTCG CCGAGATGCT CTGTTGAGCC GCCGCAATGC CCTTCACGAG 
AGAGCCATGC GTCGCCGGGC CCAGCTAGCC GATTCTTTCC ATCTGCAGCA GTTTTTCCGT 
GATTCTGATG AGCTCAAGAG TTGGGTCAAT GAGAAGATGA AAACTGCCAC AGATGAAGCT 
TATAAAGATC CATCCAACCT ACAAGGAAAA GTACAGAAGC ATCAGGCTTT TGAGGCTGAG 
CTCTCAGCAA ACCAGAGCCG AATTGATGCC TTGGAGAAAG CTGGCCAAAA GCTGATTGAT 
GTCAACCACT ATGCCAAGGA TGAAGTGGCA GCTCGTATGA ATGAGGTGAT CAGTTTGTGG 
AAGAAACTGC TAGAGGCCAC TGAACTGAAA GGAATAAAGC TTCGTGAAGC CAACCAGCAA 
CAGCAATTTA ATCGCAATGT TGAGGATATT GAATTGTGGC TATATGAAGT AGAAGGTCAC 
TTGGCTTCGG ATGATTACGG CAAAGATCTT ACCAATGTGC AGAACCTCCA GAAGAAACAT 
GCACTGCTAG AGGCAGATGT GGCTGCTCAC CAGGACCGAA TTGATGGCAT CACCATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTT GATGCAGAAA ACATCAAGAA GAAACAGGAA 
GCCCTCGTGG CTCGCTATGA GGCACTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCCGATTCTC TGCGGTTGCA GCAGCTCTTC CGGGATGTTG AGGATGAGGA GACGTGGATT 
CGAGAGAAAG AGCCCATTGC CGCATCTACC AACAGAGGTA AGGATTTAAT TGGGGTCCAG 
AATCTGCTAA AGAAACATCA AGCCTTACAA GCAGAAATTG CTGGACATGA ACCACGCATC 
AAAGCAGTTA CACAGAAGGG GAATGCCATG GTGGAGGAAG GCCATTTTGC TGCAGAGGAT 
GTGAAGGCCA AGCTTCACGA GCTGAACCAA AAGTGGGAGG CACTGAAAGC CAAAGCTTCC 
CAGCGTCGGC AGGACCTGGA GGACTCTCTG CAGGCCCAGC AGTACTTTGC TGATGCTAAC 
GAGGCTGAAT CCTGGATGCG GGAGAAGGAA CCCATTGTGG GCAGCACTGA CTATGGCAAG 
GACGAAGACT CTGCTGAGGC TCTACTGAAG AAACACGAAG CTTTGATGTC AGATCTCAGT 
GCCTACGGCA GCAGCATCCA GGCTTTGCGA GAACAAGCAC AGTCCTGCCG GCAACAAGTG 
GCCCCCACGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CTCTCTACGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAG GGAGATATCC TTACCTTACT CAACAGCACC 
AACAAGGATT GGTGGAAAGT GGAAGTGAAC GATCGTCAGG GTTTTGTGCC GGCTGCGTAC 
GTGAAGAAAT TGGACCCCGC CCAGTCAGCC TCCCGGGAGA ATCTCCTGGA GGAGCAAGGC 
AGCATAGCAC TGCGGCAGGA GCAGATTGAC AATCAGACAC GCATAACTAA GGAGGCCGGC 
AGTGTATCTC TGCGTATGAA GCAGGTGGAA GAACTATATC ATTCTCTGCT GGAACTGGGT 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TTATGTTGTT CCGTGAAGCG 
AATGAACTAC AGCAATGGAT CAATGAGAAG GAAGCCGCTC TGACAAGTGA GGAGGTCGGA 
GCAGACTTGG AGCAGGTTGA GGTGCTCCAG AAGAAGTTTG ATGACTTCCA GAAGGACCTG 
AAGGCCAATG AGTCACGGTT GAAGGACATT AACAAGGTAG CTGAAGACCT GGAGTCTGAA 
GGTCTCATGG CAGAGGAGGT GCAGGCTGTG CAACAACAGG AAGTGTATGG CATGATGCCC 
AGGGATGAAA CTGATTCCAA GACAGCCTCC CCGTGGAAGT CTGCTCGTCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTACAGCAG 
CTGGCCGAGG AACGGAGCCA GCTCTTGGGC AGCGCCCATG AAGTACAGAG GTTCCACAGA 
GATGCTGATG AAACCAAAGA ATGGATTGAA GAGAAGAATC AAGCTCTAAA CACAGACAAT 
TATGGACATG ATCTCGCCAG TGTCCAGGCC CTGCAACGCA AGCATGAGGG CTTCGAGAGG 
GACCTTGCGG CTCTCGGTGA CAAGGTAAAC TCCCTTGGTG AAACAGCAGA GCGCCTGATC 
CAGTCCCATC CCGAGTCAGC AGAAGACCTG CAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGAGCAGCC TGGGGAAACG TGCAGATCAG CGCAAGGCAA AGTTGGGTGA CTCCCACGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGGGGG 
TTGGTGTCCT CAGATGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCATT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATCGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGACAGC AGCTGTTGGC TCACGGACAC TATGCCAGCC CTGAGATCAA GCAGAAACTT 
GATATTCTTG ACCAGGAGCG TGCAGACCTG GAGAAGGCCT GGGTTCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAACT GCAGCTGTTC CATCGGGACT GTGAGCAAGC TGAGAACTGG 
ATGGCTGCCC GGGAGGCCTT CTTGAATACC GAAGACAAAG GAGACTCACT GGACAGCGTA 
GAGGCTCTGA TCAAAAAACA TGAAGACTTT GACAAAGCGA TTAACGTCCA GGAAGAGAAG 
ATTGCTGCTC TGCAGGCCTT TGCCGACCAG CTCATCGCTG CCGGCCATTA TGCCAAGGGA 
GACATTTCTA GCCGGCGCAA TGAGGTCTTG GACAGGTGGC GACGTCTGAA AGCCCAGATG 
ATTGAGAAAA GGTCAAAGCT AGGAGAATCT CAAACCCTCC AACAGTTCAG CCGGGATGTG 
GATGAGATTG AGGCTTGGAT CAGTGAAAAA TTGCAAACAG CGAGTGATGA GTCGTACAAG 
GATCCCACCA ACATCCAGAG CAAGCACCAG AAGCACCAGG CTTTTGAAGC AGAGCTGCAT 
GCCAACGCTG ACCGGATCCG TGGGGTTATC GACATGGGCA ACTCCCTCAT TGAACGTGGA 
GCCTGTGCCG GCAGTGAGGA TGCTGTCAAG GCCCGCCTGG CTGCCTTAGC TGACCAGTGG 
CAGTTCTTGG TGCAAAAGTC AGCGGAAAAG AGCCAGAAAC TGAAAGAAGC CAACAAGCAG 
CAGAACTTCA ACACAGGGAT CAAGGACTTT GACTTCTGGC TGTCTGAGGT GGAGGCCCTG 
CTGGCATCCG AAGATTATGG CAAAGACCTA GCTTCTGTGA ACAACCTGCT GAAAAAGCAT 
CAACTGCTGG AAGCAGATAT ATCTGCCCAT GAGGATCGCC TGAAGGACCT GAACAGCCAG 
GCAGACAGCC TGATGACCAG CAGTGCCTTC GACACCTCCC AAGTAAAGGA CAAGAGGGAC 
ACCATCAACG GGCGCTTCCA GAAGATCAAG AGCATGGCGG CCTCCCGGCG AGCCAAGCTG 
AATGAATCCC ATCGCCTGCA CCAGTTCTTC CGGGACATGG ATGACGAGGA GTCCTGGATC 
AAGGAGAAGA AGCTGCTGGT GGGCTCAGAG GACTACGGCC GGGACCTAAC CGGCGTGCAG 
AACCTGAGGA AGAAGCACAA GCGGCTGGAA GCAGAACTGG CTGCGCATGA GCCGGCTATT 
CAGGGTGTCC TGGACACTGG CAAGAAGCTG TCCGATGACA ACACCATCGG GAAAGAGGAG 
ATCCAGCAGC GGCTGGCGCA GTTTGTGGAG CACTGGAAAG AGCTGAAGCA GCTGGCAGCT 
GCCCGGGGTC AGCGGCTGGA AGAGTCCTTG GAATATCAGC AGTTTGTAGC CAATGTGGAA 
GAGGAAGAAG CCTGGATCAA TGAGAAAATG ACCCTGGTGG CCAGCGAAGA TTATGGCGAC 
ACTCTTGCCG CCATCCAGGG CTTACTGAAG AAACATGAAG CTTTTGAGAC AGACTTCACC 
GTCCACAAGG ATCGCGTGAA TGATGTCTGC ACCAATGGAC AAGACCTCAT TAAGAAGAAC 
AATCACCATG AGGAGAACAT CTCTTCAAAG ATGAAGGGCC TGAACGGGAA AGTGTCAGAC 
CTGGAGAAAG CTGCAGCCCA GAGAAAGGCG AAGCTGGATG AGAACTCGGC CTTCCTTCAG 
TTCAACTGGA AGGCGGACGT GGTGGAGTCC TGGATCGGTG AAAAGGAGAA CAGCTTGAAG 
ACAGATGATT ATGGCCGAGA CCTGTCTTCT GTGCAGACGC TCCTCACCAA ACAGGAAACT 
TTTGACGCTG GGCTGCAGGC CTTCCAGCAG GAAGGCATTG CCAACATCAC TGCCCTCAAA 
GATCAGCTTC TCGCCGCCAA ACACGTTCAG TCCAAGGCCA TCGAGGCCCG GCACGCCTCC 
CTCATGAAGA GGTGGAGCCA GCTTCTGGCC AACTCAGCCG CCCGCAAGAA GAAGCTTCTG 
GAGGCTCAGA GTCACTTCCG CAAGGTGGAG GACCTCTTCC TGACCTTCGC CAAAAAGGCT 
TCTGCCTTCA ACAGCTGGTT TGAAAATGCA GAGGAGGACT TAACAGACCC CGTGCGCTGC 
AACTCCTTGG AAGAAATCAA AGCTTTGCGC GAGGCCCACG ACGCCTTCCG CTCCTCCCTC 
AGCTCTGCCC AGGCTGACTT CAACCAGCTG GCCGAGCTGG ACCGCCAGAT CAAGAGCTTC 
CGCGTAGCCT CCAACCCCTA CACCTGGTTT ACCATGGAGG CCCTGGAGGA GACCTGGAGG 
AACCTACAGA AAATCATCAA GGAGAGGGAG CTGGAGCTGC AGAAGGAACA GCGGCGGCAG 
GAGGAGAACG ACAAGCTGCG CCAGGAGTTT GCCCAGCACG CCAACGCCTT CCACCAGTGG 
ATCCAAGAGA CCAGGACATA CCTCCTCGAT GGGTCCTGTA TGGTGGAAGA GTCGGGGACC 
CTCGAATCCC AGCTTGAAGC TACCAAACGC AAGCACCAGG AAATCCGAGC CATGAGAAGT 
CAGCTCAAAA AGATCGAGGA CCTGGGGGCC GCCATGGAGG AGGCCCTCAT CCTGGACAAC 
AAGTACACGG AGCACAGCAC CGTGGGCCTC GCCCAGCAGT GGGACCAGCT GGACCAGCTG 
GGCATGCGCA TGCAGCACAA CCTGGAGCAG CAGATCCAGG CCAGGAACAC AACAGGTGTG 
ACTGAGGAGG CCCTCAAAGA ATTCAGCATG ATGTTTAAAC ACTTTGACAA GGACAAGTCT 
GGCAGGCTGA ACCATCAGGA GTTCAAATCT TGCCTGCGCT CCCTGGGCTA TGACCTGCCC 
ATGGTGGAGG AAGGGGAACC TGACCCTGAG TTCGAGGCAA TCCTGGACAC GGTGGATCCG 
AACAGAGATG GCCATGTCTC CTTGCAAGAA TACATGGCTT TCATGATCAG CCGCGAAACT 
GAGAACGTCA AGTCCAGCGA GGAGATTGAG AGCGCCTTCC GGGCCCTCAG CTCAGAGGGA 
AAGCCTTACG TGACCAAGGA GGAGCTCTAC CAGAACCTGA CCCGGGAACA AGCCGACTAC 
TGCGTCTCCC ACATGAAGCC CTACGTGGAC GGCAAGGGCC GCGAGCTCCC CACCGCGTTC 
GACTACGTGG AGTTCACCCG CTCGCTTTTC GTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( NM_001375311.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001362240.1 Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant 7, mRNA.

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CloneID	OHu108711
Clone ID Related Accession (Same CDS sequence)	NM_001375311.2 , NM_001130438.3
Accession Version	NM_001375311.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7434bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	spectrin alpha chain, non-erythrocytic 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL356481.16. On Oct 23, 2019 this sequence version replaced NM_001375311.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.70190.1, SRR1803615.126969.1 [ECO:0000332] ##Evidence-Data-END##

ATGGACCCAA GTGGGGTCAA AGTGCTGGAA ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTAG ACCGATACCA CCGCTTCAAG GAACTCTCAA CCCTTAGGCG TCAGAAGCTG 
GAAGATTCCT ATCGATTCCA GTTCTTTCAA AGAGATGCTG AAGAGCTGGA GAAATGGATA 
CAGGAAAAAC TTCAGATTGC ATCTGATGAG AATTATAAAG ACCCAACCAA CTTGCAGGGA 
AAGCTTCAGA AGCATCAAGC ATTTGAAGCT GAAGTGCAGG CCAACTCAGG AGCCATTGTT 
AAGCTGGATG AAACTGGAAA CCTGATGATC TCAGAAGGGC ATTTTGCATC TGAAACCATA 
CGGACCCGTT TGATGGAGCT GCACCGCCAG TGGGAATTAC TTTTGGAGAA GATGCGAGAA 
AAAGGAATCA AACTGCTGCA GGCCCAGAAG TTGGTGCAGT ACTTACGAGA ATGTGAGGAC 
GTGATGGACT GGATCAATGA CAAGGAAGCA ATTGTTACTT CTGAAGAGCT GGGCCAGGAT 
CTGGAGCATG TAGAGGTTTT ACAGAAGAAA TTTGAAGAGT TTCAAACAGA TATGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTGAACCAG TTTGCTGCCA AACTCATACA GGAGCAGCAC 
CCTGAGGAGG AACTGATCAA GACTAAGCAG GATGAAGTCA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CTCTGCAGAG GCAGGGGAAG CTCTTTGGGG CAGCAGAAGT TCAGCGCTTT 
AACAGGGATG TGGATGAGAC TATCAGTTGG ATTAAGGAAA AGGAGCAGTT AATGGCCTCT 
GATGATTTTG GCCGAGACCT GGCAAGTGTT CAGGCTCTGC TTCGGAAGCA CGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAAGACAAG GTCAAAGCCC TGTGTGCTGA GGCTGACCGC 
CTGCAACAGT CCCACCCTCT GAGTGCAACA CAGATTCAAG TGAAGCGAGA GGAACTGATT 
ACAAACTGGG AGCAGATCCG CACCTTGGCG GCAGAGAGAC ATGCACGGCT CAATGATTCA 
TACAGGCTTC AACGCTTCCT TGCTGACTTC CGTGACCTCA CCAGCTGGGT GACTGAGATG 
AAAGCCCTCA TCAATGCAGA TGAGCTTGCC AGTGATGTGG CTGGGGCTGA AGCCCTGCTA 
GATAGACACC AAGAGCACAA GGGTGAAATT GATGCCCATG AAGACAGCTT CAAATCTGCA 
GATGAATCTG GACAGGCACT GCTTGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGACCG TCCTTTCCGA GGAGAGAGCG GCGCTGCTGG AGCTGTGGGA GCTGCGCAGG 
CAGCAGTACG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GGGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAATGAAG ACTTGGGAGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTTAA GAAGCACGAA GACTTTGAGA AATCCCTTAG TGCCCAGGAG 
GAAAAGATTA CAGCATTAGA TGAATTTGCA ACCAAGCTAA TTCAGAACAA CCACTATGCA 
ATGGAAGATG TGGCCACTCG CCGAGATGCT CTGTTGAGCC GCCGCAATGC CCTTCACGAG 
AGAGCCATGC GTCGCCGGGC CCAGCTAGCC GATTCTTTCC ATCTGCAGCA GTTTTTCCGT 
GATTCTGATG AGCTCAAGAG TTGGGTCAAT GAGAAGATGA AAACTGCCAC AGATGAAGCT 
TATAAAGATC CATCCAACCT ACAAGGAAAA GTACAGAAGC ATCAGGCTTT TGAGGCTGAG 
CTCTCAGCAA ACCAGAGCCG AATTGATGCC TTGGAGAAAG CTGGCCAAAA GCTGATTGAT 
GTCAACCACT ATGCCAAGGA TGAAGTGGCA GCTCGTATGA ATGAGGTGAT CAGTTTGTGG 
AAGAAACTGC TAGAGGCCAC TGAACTGAAA GGAATAAAGC TTCGTGAAGC CAACCAGCAA 
CAGCAATTTA ATCGCAATGT TGAGGATATT GAATTGTGGC TATATGAAGT AGAAGGTCAC 
TTGGCTTCGG ATGATTACGG CAAAGATCTT ACCAATGTGC AGAACCTCCA GAAGAAACAT 
GCACTGCTAG AGGCAGATGT GGCTGCTCAC CAGGACCGAA TTGATGGCAT CACCATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTT GATGCAGAAA ACATCAAGAA GAAACAGGAA 
GCCCTCGTGG CTCGCTATGA GGCACTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCCGATTCTC TGCGGTTGCA GCAGCTCTTC CGGGATGTTG AGGATGAGGA GACGTGGATT 
CGAGAGAAAG AGCCCATTGC CGCATCTACC AACAGAGGTA AGGATTTAAT TGGGGTCCAG 
AATCTGCTAA AGAAACATCA AGCCTTACAA GCAGAAATTG CTGGACATGA ACCACGCATC 
AAAGCAGTTA CACAGAAGGG GAATGCCATG GTGGAGGAAG GCCATTTTGC TGCAGAGGAT 
GTGAAGGCCA AGCTTCACGA GCTGAACCAA AAGTGGGAGG CACTGAAAGC CAAAGCTTCC 
CAGCGTCGGC AGGACCTGGA GGACTCTCTG CAGGCCCAGC AGTACTTTGC TGATGCTAAC 
GAGGCTGAAT CCTGGATGCG GGAGAAGGAA CCCATTGTGG GCAGCACTGA CTATGGCAAG 
GACGAAGACT CTGCTGAGGC TCTACTGAAG AAACACGAAG CTTTGATGTC AGATCTCAGT 
GCCTACGGCA GCAGCATCCA GGCTTTGCGA GAACAAGCAC AGTCCTGCCG GCAACAAGTG 
GCCCCCACGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CTCTCTACGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAG GGAGATATCC TTACCTTACT CAACAGCACC 
AACAAGGATT GGTGGAAAGT GGAAGTGAAC GATCGTCAGG GTTTTGTGCC GGCTGCGTAC 
GTGAAGAAAT TGGACCCCGC CCAGTCAGCC TCCCGGGAGA ATCTCCTGGA GGAGCAAGGC 
AGCATAGCAC TGCGGCAGGA GCAGATTGAC AATCAGACAC GCATAACTAA GGAGGCCGGC 
AGTGTATCTC TGCGTATGAA GCAGGTGGAA GAACTATATC ATTCTCTGCT GGAACTGGGT 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TTATGTTGTT CCGTGAAGCG 
AATGAACTAC AGCAATGGAT CAATGAGAAG GAAGCCGCTC TGACAAGTGA GGAGGTCGGA 
GCAGACTTGG AGCAGGTTGA GGTGCTCCAG AAGAAGTTTG ATGACTTCCA GAAGGACCTG 
AAGGCCAATG AGTCACGGTT GAAGGACATT AACAAGGTAG CTGAAGACCT GGAGTCTGAA 
GGTCTCATGG CAGAGGAGGT GCAGGCTGTG CAACAACAGG AAGTGTATGG CATGATGCCC 
AGGGATGAAA CTGATTCCAA GACAGCCTCC CCGTGGAAGT CTGCTCGTCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTACAGCAG 
CTGGCCGAGG AACGGAGCCA GCTCTTGGGC AGCGCCCATG AAGTACAGAG GTTCCACAGA 
GATGCTGATG AAACCAAAGA ATGGATTGAA GAGAAGAATC AAGCTCTAAA CACAGACAAT 
TATGGACATG ATCTCGCCAG TGTCCAGGCC CTGCAACGCA AGCATGAGGG CTTCGAGAGG 
GACCTTGCGG CTCTCGGTGA CAAGGTAAAC TCCCTTGGTG AAACAGCAGA GCGCCTGATC 
CAGTCCCATC CCGAGTCAGC AGAAGACCTG CAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGAGCAGCC TGGGGAAACG TGCAGATCAG CGCAAGGCAA AGTTGGGTGA CTCCCACGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGGGGG 
TTGGTGTCCT CAGATGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCATT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATCGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGACAGC AGCTGTTGGC TCACGGACAC TATGCCAGCC CTGAGATCAA GCAGAAACTT 
GATATTCTTG ACCAGGAGCG TGCAGACCTG GAGAAGGCCT GGGTTCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAACT GCAGCTGTTC CATCGGGACT GTGAGCAAGC TGAGAACTGG 
ATGGCTGCCC GGGAGGCCTT CTTGAATACC GAAGACAAAG GAGACTCACT GGACAGCGTA 
GAGGCTCTGA TCAAAAAACA TGAAGACTTT GACAAAGCGA TTAACGTCCA GGAAGAGAAG 
ATTGCTGCTC TGCAGGCCTT TGCCGACCAG CTCATCGCTG CCGGCCATTA TGCCAAGGGA 
GACATTTCTA GCCGGCGCAA TGAGGTCTTG GACAGGTGGC GACGTCTGAA AGCCCAGATG 
ATTGAGAAAA GGTCAAAGCT AGGAGAATCT CAAACCCTCC AACAGTTCAG CCGGGATGTG 
GATGAGATTG AGGCTTGGAT CAGTGAAAAA TTGCAAACAG CGAGTGATGA GTCGTACAAG 
GATCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ACCAGAAGCA CCAGGCTTTT 
GAAGCAGAGC TGCATGCCAA CGCTGACCGG ATCCGTGGGG TTATCGACAT GGGCAACTCC 
CTCATTGAAC GTGGAGCCTG TGCCGGCAGT GAGGATGCTG TCAAGGCCCG CCTGGCTGCC 
TTAGCTGACC AGTGGCAGTT CTTGGTGCAA AAGTCAGCGG AAAAGAGCCA GAAACTGAAA 
GAAGCCAACA AGCAGCAGAA CTTCAACACA GGGATCAAGG ACTTTGACTT CTGGCTGTCT 
GAGGTGGAGG CCCTGCTGGC ATCCGAAGAT TATGGCAAAG ACCTAGCTTC TGTGAACAAC 
CTGCTGAAAA AGCATCAACT GCTGGAAGCA GATATATCTG CCCATGAGGA TCGCCTGAAG 
GACCTGAACA GCCAGGCAGA CAGCCTGATG ACCAGCAGTG CCTTCGACAC CTCCCAAGTA 
AAGGACAAGA GGGACACCAT CAACGGGCGC TTCCAGAAGA TCAAGAGCAT GGCGGCCTCC 
CGGCGAGCCA AGCTGAATGA ATCCCATCGC CTGCACCAGT TCTTCCGGGA CATGGATGAC 
GAGGAGTCCT GGATCAAGGA GAAGAAGCTG CTGGTGGGCT CAGAGGACTA CGGCCGGGAC 
CTAACCGGCG TGCAGAACCT GAGGAAGAAG CACAAGCGGC TGGAAGCAGA ACTGGCTGCG 
CATGAGCCGG CTATTCAGGG TGTCCTGGAC ACTGGCAAGA AGCTGTCCGA TGACAACACC 
ATCGGGAAAG AGGAGATCCA GCAGCGGCTG GCGCAGTTTG TGGAGCACTG GAAAGAGCTG 
AAGCAGCTGG CAGCTGCCCG GGGTCAGCGG CTGGAAGAGT CCTTGGAATA TCAGCAGTTT 
GTAGCCAATG TGGAAGAGGA AGAAGCCTGG ATCAATGAGA AAATGACCCT GGTGGCCAGC 
GAAGATTATG GCGACACTCT TGCCGCCATC CAGGGCTTAC TGAAGAAACA TGAAGCTTTT 
GAGACAGACT TCACCGTCCA CAAGGATCGC GTGAATGATG TCTGCACCAA TGGACAAGAC 
CTCATTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CAAAGATGAA GGGCCTGAAC 
GGGAAAGTGT CAGACCTGGA GAAAGCTGCA GCCCAGAGAA AGGCGAAGCT GGATGAGAAC 
TCGGCCTTCC TTCAGTTCAA CTGGAAGGCG GACGTGGTGG AGTCCTGGAT CGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGC CGAGACCTGT CTTCTGTGCA GACGCTCCTC 
ACCAAACAGG AAACTTTTGA CGCTGGGCTG CAGGCCTTCC AGCAGGAAGG CATTGCCAAC 
ATCACTGCCC TCAAAGATCA GCTTCTCGCC GCCAAACACG TTCAGTCCAA GGCCATCGAG 
GCCCGGCACG CCTCCCTCAT GAAGAGGTGG AGCCAGCTTC TGGCCAACTC AGCCGCCCGC 
AAGAAGAAGC TTCTGGAGGC TCAGAGTCAC TTCCGCAAGG TGGAGGACCT CTTCCTGACC 
TTCGCCAAAA AGGCTTCTGC CTTCAACAGC TGGTTTGAAA ATGCAGAGGA GGACTTAACA 
GACCCCGTGC GCTGCAACTC CTTGGAAGAA ATCAAAGCTT TGCGCGAGGC CCACGACGCC 
TTCCGCTCCT CCCTCAGCTC TGCCCAGGCT GACTTCAACC AGCTGGCCGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT AGCCTCCAAC CCCTACACCT GGTTTACCAT GGAGGCCCTG 
GAGGAGACCT GGAGGAACCT ACAGAAAATC ATCAAGGAGA GGGAGCTGGA GCTGCAGAAG 
GAACAGCGGC GGCAGGAGGA GAACGACAAG CTGCGCCAGG AGTTTGCCCA GCACGCCAAC 
GCCTTCCACC AGTGGATCCA AGAGACCAGG ACATACCTCC TCGATGGGTC CTGTATGGTG 
GAAGAGTCGG GGACCCTCGA ATCCCAGCTT GAAGCTACCA AACGCAAGCA CCAGGAAATC 
CGAGCCATGA GAAGTCAGCT CAAAAAGATC GAGGACCTGG GGGCCGCCAT GGAGGAGGCC 
CTCATCCTGG ACAACAAGTA CACGGAGCAC AGCACCGTGG GCCTCGCCCA GCAGTGGGAC 
CAGCTGGACC AGCTGGGCAT GCGCATGCAG CACAACCTGG AGCAGCAGAT CCAGGCCAGG 
AACACAACAG GTGTGACTGA GGAGGCCCTC AAAGAATTCA GCATGATGTT TAAACACTTT 
GACAAGGACA AGTCTGGCAG GCTGAACCAT CAGGAGTTCA AATCTTGCCT GCGCTCCCTG 
GGCTATGACC TGCCCATGGT GGAGGAAGGG GAACCTGACC CTGAGTTCGA GGCAATCCTG 
GACACGGTGG ATCCGAACAG AGATGGCCAT GTCTCCTTGC AAGAATACAT GGCTTTCATG 
ATCAGCCGCG AAACTGAGAA CGTCAAGTCC AGCGAGGAGA TTGAGAGCGC CTTCCGGGCC 
CTCAGCTCAG AGGGAAAGCC TTACGTGACC AAGGAGGAGC TCTACCAGAA CCTGACCCGG 
GAACAAGCCG ACTACTGCGT CTCCCACATG AAGCCCTACG TGGACGGCAA GGGCCGCGAG 
CTCCCCACCG CGTTCGACTA CGTGGAGTTC ACCCGCTCGC TTTTCGTGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001362240.1
CDS	729..8162
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELASDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMRRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPTDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRITKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGMMPRDETDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKQKLDILDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAAGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINGRFQKIKSMAASRRAKLNESHRLHQFFRDMDDEESWIKEKKLLVGSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHASLMKRWSQLLANSAARKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	NM_001375311.2
Organism	Homo sapiens(human)
Definition	Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001375311.2

ORF Insert Sequence:

ATGGACCCAA GTGGGGTCAA AGTGCTGGAA ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTAG ACCGATACCA CCGCTTCAAG GAACTCTCAA CCCTTAGGCG TCAGAAGCTG 
GAAGATTCCT ATCGATTCCA GTTCTTTCAA AGAGATGCTG AAGAGCTGGA GAAATGGATA 
CAGGAAAAAC TTCAGATTGC ATCTGATGAG AATTATAAAG ACCCAACCAA CTTGCAGGGA 
AAGCTTCAGA AGCATCAAGC ATTTGAAGCT GAAGTGCAGG CCAACTCAGG AGCCATTGTT 
AAGCTGGATG AAACTGGAAA CCTGATGATC TCAGAAGGGC ATTTTGCATC TGAAACCATA 
CGGACCCGTT TGATGGAGCT GCACCGCCAG TGGGAATTAC TTTTGGAGAA GATGCGAGAA 
AAAGGAATCA AACTGCTGCA GGCCCAGAAG TTGGTGCAGT ACTTACGAGA ATGTGAGGAC 
GTGATGGACT GGATCAATGA CAAGGAAGCA ATTGTTACTT CTGAAGAGCT GGGCCAGGAT 
CTGGAGCATG TAGAGGTTTT ACAGAAGAAA TTTGAAGAGT TTCAAACAGA TATGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTGAACCAG TTTGCTGCCA AACTCATACA GGAGCAGCAC 
CCTGAGGAGG AACTGATCAA GACTAAGCAG GATGAAGTCA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CTCTGCAGAG GCAGGGGAAG CTCTTTGGGG CAGCAGAAGT TCAGCGCTTT 
AACAGGGATG TGGATGAGAC TATCAGTTGG ATTAAGGAAA AGGAGCAGTT AATGGCCTCT 
GATGATTTTG GCCGAGACCT GGCAAGTGTT CAGGCTCTGC TTCGGAAGCA CGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAAGACAAG GTCAAAGCCC TGTGTGCTGA GGCTGACCGC 
CTGCAACAGT CCCACCCTCT GAGTGCAACA CAGATTCAAG TGAAGCGAGA GGAACTGATT 
ACAAACTGGG AGCAGATCCG CACCTTGGCG GCAGAGAGAC ATGCACGGCT CAATGATTCA 
TACAGGCTTC AACGCTTCCT TGCTGACTTC CGTGACCTCA CCAGCTGGGT GACTGAGATG 
AAAGCCCTCA TCAATGCAGA TGAGCTTGCC AGTGATGTGG CTGGGGCTGA AGCCCTGCTA 
GATAGACACC AAGAGCACAA GGGTGAAATT GATGCCCATG AAGACAGCTT CAAATCTGCA 
GATGAATCTG GACAGGCACT GCTTGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGACCG TCCTTTCCGA GGAGAGAGCG GCGCTGCTGG AGCTGTGGGA GCTGCGCAGG 
CAGCAGTACG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GGGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAATGAAG ACTTGGGAGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTTAA GAAGCACGAA GACTTTGAGA AATCCCTTAG TGCCCAGGAG 
GAAAAGATTA CAGCATTAGA TGAATTTGCA ACCAAGCTAA TTCAGAACAA CCACTATGCA 
ATGGAAGATG TGGCCACTCG CCGAGATGCT CTGTTGAGCC GCCGCAATGC CCTTCACGAG 
AGAGCCATGC GTCGCCGGGC CCAGCTAGCC GATTCTTTCC ATCTGCAGCA GTTTTTCCGT 
GATTCTGATG AGCTCAAGAG TTGGGTCAAT GAGAAGATGA AAACTGCCAC AGATGAAGCT 
TATAAAGATC CATCCAACCT ACAAGGAAAA GTACAGAAGC ATCAGGCTTT TGAGGCTGAG 
CTCTCAGCAA ACCAGAGCCG AATTGATGCC TTGGAGAAAG CTGGCCAAAA GCTGATTGAT 
GTCAACCACT ATGCCAAGGA TGAAGTGGCA GCTCGTATGA ATGAGGTGAT CAGTTTGTGG 
AAGAAACTGC TAGAGGCCAC TGAACTGAAA GGAATAAAGC TTCGTGAAGC CAACCAGCAA 
CAGCAATTTA ATCGCAATGT TGAGGATATT GAATTGTGGC TATATGAAGT AGAAGGTCAC 
TTGGCTTCGG ATGATTACGG CAAAGATCTT ACCAATGTGC AGAACCTCCA GAAGAAACAT 
GCACTGCTAG AGGCAGATGT GGCTGCTCAC CAGGACCGAA TTGATGGCAT CACCATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTT GATGCAGAAA ACATCAAGAA GAAACAGGAA 
GCCCTCGTGG CTCGCTATGA GGCACTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCCGATTCTC TGCGGTTGCA GCAGCTCTTC CGGGATGTTG AGGATGAGGA GACGTGGATT 
CGAGAGAAAG AGCCCATTGC CGCATCTACC AACAGAGGTA AGGATTTAAT TGGGGTCCAG 
AATCTGCTAA AGAAACATCA AGCCTTACAA GCAGAAATTG CTGGACATGA ACCACGCATC 
AAAGCAGTTA CACAGAAGGG GAATGCCATG GTGGAGGAAG GCCATTTTGC TGCAGAGGAT 
GTGAAGGCCA AGCTTCACGA GCTGAACCAA AAGTGGGAGG CACTGAAAGC CAAAGCTTCC 
CAGCGTCGGC AGGACCTGGA GGACTCTCTG CAGGCCCAGC AGTACTTTGC TGATGCTAAC 
GAGGCTGAAT CCTGGATGCG GGAGAAGGAA CCCATTGTGG GCAGCACTGA CTATGGCAAG 
GACGAAGACT CTGCTGAGGC TCTACTGAAG AAACACGAAG CTTTGATGTC AGATCTCAGT 
GCCTACGGCA GCAGCATCCA GGCTTTGCGA GAACAAGCAC AGTCCTGCCG GCAACAAGTG 
GCCCCCACGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CTCTCTACGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAG GGAGATATCC TTACCTTACT CAACAGCACC 
AACAAGGATT GGTGGAAAGT GGAAGTGAAC GATCGTCAGG GTTTTGTGCC GGCTGCGTAC 
GTGAAGAAAT TGGACCCCGC CCAGTCAGCC TCCCGGGAGA ATCTCCTGGA GGAGCAAGGC 
AGCATAGCAC TGCGGCAGGA GCAGATTGAC AATCAGACAC GCATAACTAA GGAGGCCGGC 
AGTGTATCTC TGCGTATGAA GCAGGTGGAA GAACTATATC ATTCTCTGCT GGAACTGGGT 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TTATGTTGTT CCGTGAAGCG 
AATGAACTAC AGCAATGGAT CAATGAGAAG GAAGCCGCTC TGACAAGTGA GGAGGTCGGA 
GCAGACTTGG AGCAGGTTGA GGTGCTCCAG AAGAAGTTTG ATGACTTCCA GAAGGACCTG 
AAGGCCAATG AGTCACGGTT GAAGGACATT AACAAGGTAG CTGAAGACCT GGAGTCTGAA 
GGTCTCATGG CAGAGGAGGT GCAGGCTGTG CAACAACAGG AAGTGTATGG CATGATGCCC 
AGGGATGAAA CTGATTCCAA GACAGCCTCC CCGTGGAAGT CTGCTCGTCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTACAGCAG 
CTGGCCGAGG AACGGAGCCA GCTCTTGGGC AGCGCCCATG AAGTACAGAG GTTCCACAGA 
GATGCTGATG AAACCAAAGA ATGGATTGAA GAGAAGAATC AAGCTCTAAA CACAGACAAT 
TATGGACATG ATCTCGCCAG TGTCCAGGCC CTGCAACGCA AGCATGAGGG CTTCGAGAGG 
GACCTTGCGG CTCTCGGTGA CAAGGTAAAC TCCCTTGGTG AAACAGCAGA GCGCCTGATC 
CAGTCCCATC CCGAGTCAGC AGAAGACCTG CAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGAGCAGCC TGGGGAAACG TGCAGATCAG CGCAAGGCAA AGTTGGGTGA CTCCCACGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGGGGG 
TTGGTGTCCT CAGATGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCATT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATCGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGACAGC AGCTGTTGGC TCACGGACAC TATGCCAGCC CTGAGATCAA GCAGAAACTT 
GATATTCTTG ACCAGGAGCG TGCAGACCTG GAGAAGGCCT GGGTTCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAACT GCAGCTGTTC CATCGGGACT GTGAGCAAGC TGAGAACTGG 
ATGGCTGCCC GGGAGGCCTT CTTGAATACC GAAGACAAAG GAGACTCACT GGACAGCGTA 
GAGGCTCTGA TCAAAAAACA TGAAGACTTT GACAAAGCGA TTAACGTCCA GGAAGAGAAG 
ATTGCTGCTC TGCAGGCCTT TGCCGACCAG CTCATCGCTG CCGGCCATTA TGCCAAGGGA 
GACATTTCTA GCCGGCGCAA TGAGGTCTTG GACAGGTGGC GACGTCTGAA AGCCCAGATG 
ATTGAGAAAA GGTCAAAGCT AGGAGAATCT CAAACCCTCC AACAGTTCAG CCGGGATGTG 
GATGAGATTG AGGCTTGGAT CAGTGAAAAA TTGCAAACAG CGAGTGATGA GTCGTACAAG 
GATCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ACCAGAAGCA CCAGGCTTTT 
GAAGCAGAGC TGCATGCCAA CGCTGACCGG ATCCGTGGGG TTATCGACAT GGGCAACTCC 
CTCATTGAAC GTGGAGCCTG TGCCGGCAGT GAGGATGCTG TCAAGGCCCG CCTGGCTGCC 
TTAGCTGACC AGTGGCAGTT CTTGGTGCAA AAGTCAGCGG AAAAGAGCCA GAAACTGAAA 
GAAGCCAACA AGCAGCAGAA CTTCAACACA GGGATCAAGG ACTTTGACTT CTGGCTGTCT 
GAGGTGGAGG CCCTGCTGGC ATCCGAAGAT TATGGCAAAG ACCTAGCTTC TGTGAACAAC 
CTGCTGAAAA AGCATCAACT GCTGGAAGCA GATATATCTG CCCATGAGGA TCGCCTGAAG 
GACCTGAACA GCCAGGCAGA CAGCCTGATG ACCAGCAGTG CCTTCGACAC CTCCCAAGTA 
AAGGACAAGA GGGACACCAT CAACGGGCGC TTCCAGAAGA TCAAGAGCAT GGCGGCCTCC 
CGGCGAGCCA AGCTGAATGA ATCCCATCGC CTGCACCAGT TCTTCCGGGA CATGGATGAC 
GAGGAGTCCT GGATCAAGGA GAAGAAGCTG CTGGTGGGCT CAGAGGACTA CGGCCGGGAC 
CTAACCGGCG TGCAGAACCT GAGGAAGAAG CACAAGCGGC TGGAAGCAGA ACTGGCTGCG 
CATGAGCCGG CTATTCAGGG TGTCCTGGAC ACTGGCAAGA AGCTGTCCGA TGACAACACC 
ATCGGGAAAG AGGAGATCCA GCAGCGGCTG GCGCAGTTTG TGGAGCACTG GAAAGAGCTG 
AAGCAGCTGG CAGCTGCCCG GGGTCAGCGG CTGGAAGAGT CCTTGGAATA TCAGCAGTTT 
GTAGCCAATG TGGAAGAGGA AGAAGCCTGG ATCAATGAGA AAATGACCCT GGTGGCCAGC 
GAAGATTATG GCGACACTCT TGCCGCCATC CAGGGCTTAC TGAAGAAACA TGAAGCTTTT 
GAGACAGACT TCACCGTCCA CAAGGATCGC GTGAATGATG TCTGCACCAA TGGACAAGAC 
CTCATTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CAAAGATGAA GGGCCTGAAC 
GGGAAAGTGT CAGACCTGGA GAAAGCTGCA GCCCAGAGAA AGGCGAAGCT GGATGAGAAC 
TCGGCCTTCC TTCAGTTCAA CTGGAAGGCG GACGTGGTGG AGTCCTGGAT CGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGC CGAGACCTGT CTTCTGTGCA GACGCTCCTC 
ACCAAACAGG AAACTTTTGA CGCTGGGCTG CAGGCCTTCC AGCAGGAAGG CATTGCCAAC 
ATCACTGCCC TCAAAGATCA GCTTCTCGCC GCCAAACACG TTCAGTCCAA GGCCATCGAG 
GCCCGGCACG CCTCCCTCAT GAAGAGGTGG AGCCAGCTTC TGGCCAACTC AGCCGCCCGC 
AAGAAGAAGC TTCTGGAGGC TCAGAGTCAC TTCCGCAAGG TGGAGGACCT CTTCCTGACC 
TTCGCCAAAA AGGCTTCTGC CTTCAACAGC TGGTTTGAAA ATGCAGAGGA GGACTTAACA 
GACCCCGTGC GCTGCAACTC CTTGGAAGAA ATCAAAGCTT TGCGCGAGGC CCACGACGCC 
TTCCGCTCCT CCCTCAGCTC TGCCCAGGCT GACTTCAACC AGCTGGCCGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT AGCCTCCAAC CCCTACACCT GGTTTACCAT GGAGGCCCTG 
GAGGAGACCT GGAGGAACCT ACAGAAAATC ATCAAGGAGA GGGAGCTGGA GCTGCAGAAG 
GAACAGCGGC GGCAGGAGGA GAACGACAAG CTGCGCCAGG AGTTTGCCCA GCACGCCAAC 
GCCTTCCACC AGTGGATCCA AGAGACCAGG ACATACCTCC TCGATGGGTC CTGTATGGTG 
GAAGAGTCGG GGACCCTCGA ATCCCAGCTT GAAGCTACCA AACGCAAGCA CCAGGAAATC 
CGAGCCATGA GAAGTCAGCT CAAAAAGATC GAGGACCTGG GGGCCGCCAT GGAGGAGGCC 
CTCATCCTGG ACAACAAGTA CACGGAGCAC AGCACCGTGG GCCTCGCCCA GCAGTGGGAC 
CAGCTGGACC AGCTGGGCAT GCGCATGCAG CACAACCTGG AGCAGCAGAT CCAGGCCAGG 
AACACAACAG GTGTGACTGA GGAGGCCCTC AAAGAATTCA GCATGATGTT TAAACACTTT 
GACAAGGACA AGTCTGGCAG GCTGAACCAT CAGGAGTTCA AATCTTGCCT GCGCTCCCTG 
GGCTATGACC TGCCCATGGT GGAGGAAGGG GAACCTGACC CTGAGTTCGA GGCAATCCTG 
GACACGGTGG ATCCGAACAG AGATGGCCAT GTCTCCTTGC AAGAATACAT GGCTTTCATG 
ATCAGCCGCG AAACTGAGAA CGTCAAGTCC AGCGAGGAGA TTGAGAGCGC CTTCCGGGCC 
CTCAGCTCAG AGGGAAAGCC TTACGTGACC AAGGAGGAGC TCTACCAGAA CCTGACCCGG 
GAACAAGCCG ACTACTGCGT CTCCCACATG AAGCCCTACG TGGACGGCAA GGGCCGCGAG 
CTCCCCACCG CGTTCGACTA CGTGGAGTTC ACCCGCTCGC TTTTCGTGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( NM_001130438.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001123910.1 Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant 1, mRNA.

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CloneID	OHu108711
Clone ID Related Accession (Same CDS sequence)	NM_001375311.2 , NM_001130438.3
Accession Version	NM_001130438.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7434bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	spectrin alpha chain, non-erythrocytic 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL356481.16. On Jun 2, 2019 this sequence version replaced NM_001130438.2. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.75589.1, SRR1803617.253053.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGACCCAA GTGGGGTCAA AGTGCTGGAA ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTAG ACCGATACCA CCGCTTCAAG GAACTCTCAA CCCTTAGGCG TCAGAAGCTG 
GAAGATTCCT ATCGATTCCA GTTCTTTCAA AGAGATGCTG AAGAGCTGGA GAAATGGATA 
CAGGAAAAAC TTCAGATTGC ATCTGATGAG AATTATAAAG ACCCAACCAA CTTGCAGGGA 
AAGCTTCAGA AGCATCAAGC ATTTGAAGCT GAAGTGCAGG CCAACTCAGG AGCCATTGTT 
AAGCTGGATG AAACTGGAAA CCTGATGATC TCAGAAGGGC ATTTTGCATC TGAAACCATA 
CGGACCCGTT TGATGGAGCT GCACCGCCAG TGGGAATTAC TTTTGGAGAA GATGCGAGAA 
AAAGGAATCA AACTGCTGCA GGCCCAGAAG TTGGTGCAGT ACTTACGAGA ATGTGAGGAC 
GTGATGGACT GGATCAATGA CAAGGAAGCA ATTGTTACTT CTGAAGAGCT GGGCCAGGAT 
CTGGAGCATG TAGAGGTTTT ACAGAAGAAA TTTGAAGAGT TTCAAACAGA TATGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTGAACCAG TTTGCTGCCA AACTCATACA GGAGCAGCAC 
CCTGAGGAGG AACTGATCAA GACTAAGCAG GATGAAGTCA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CTCTGCAGAG GCAGGGGAAG CTCTTTGGGG CAGCAGAAGT TCAGCGCTTT 
AACAGGGATG TGGATGAGAC TATCAGTTGG ATTAAGGAAA AGGAGCAGTT AATGGCCTCT 
GATGATTTTG GCCGAGACCT GGCAAGTGTT CAGGCTCTGC TTCGGAAGCA CGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAAGACAAG GTCAAAGCCC TGTGTGCTGA GGCTGACCGC 
CTGCAACAGT CCCACCCTCT GAGTGCAACA CAGATTCAAG TGAAGCGAGA GGAACTGATT 
ACAAACTGGG AGCAGATCCG CACCTTGGCG GCAGAGAGAC ATGCACGGCT CAATGATTCA 
TACAGGCTTC AACGCTTCCT TGCTGACTTC CGTGACCTCA CCAGCTGGGT GACTGAGATG 
AAAGCCCTCA TCAATGCAGA TGAGCTTGCC AGTGATGTGG CTGGGGCTGA AGCCCTGCTA 
GATAGACACC AAGAGCACAA GGGTGAAATT GATGCCCATG AAGACAGCTT CAAATCTGCA 
GATGAATCTG GACAGGCACT GCTTGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGACCG TCCTTTCCGA GGAGAGAGCG GCGCTGCTGG AGCTGTGGGA GCTGCGCAGG 
CAGCAGTACG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GGGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAATGAAG ACTTGGGAGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTTAA GAAGCACGAA GACTTTGAGA AATCCCTTAG TGCCCAGGAG 
GAAAAGATTA CAGCATTAGA TGAATTTGCA ACCAAGCTAA TTCAGAACAA CCACTATGCA 
ATGGAAGATG TGGCCACTCG CCGAGATGCT CTGTTGAGCC GCCGCAATGC CCTTCACGAG 
AGAGCCATGC GTCGCCGGGC CCAGCTAGCC GATTCTTTCC ATCTGCAGCA GTTTTTCCGT 
GATTCTGATG AGCTCAAGAG TTGGGTCAAT GAGAAGATGA AAACTGCCAC AGATGAAGCT 
TATAAAGATC CATCCAACCT ACAAGGAAAA GTACAGAAGC ATCAGGCTTT TGAGGCTGAG 
CTCTCAGCAA ACCAGAGCCG AATTGATGCC TTGGAGAAAG CTGGCCAAAA GCTGATTGAT 
GTCAACCACT ATGCCAAGGA TGAAGTGGCA GCTCGTATGA ATGAGGTGAT CAGTTTGTGG 
AAGAAACTGC TAGAGGCCAC TGAACTGAAA GGAATAAAGC TTCGTGAAGC CAACCAGCAA 
CAGCAATTTA ATCGCAATGT TGAGGATATT GAATTGTGGC TATATGAAGT AGAAGGTCAC 
TTGGCTTCGG ATGATTACGG CAAAGATCTT ACCAATGTGC AGAACCTCCA GAAGAAACAT 
GCACTGCTAG AGGCAGATGT GGCTGCTCAC CAGGACCGAA TTGATGGCAT CACCATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTT GATGCAGAAA ACATCAAGAA GAAACAGGAA 
GCCCTCGTGG CTCGCTATGA GGCACTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCCGATTCTC TGCGGTTGCA GCAGCTCTTC CGGGATGTTG AGGATGAGGA GACGTGGATT 
CGAGAGAAAG AGCCCATTGC CGCATCTACC AACAGAGGTA AGGATTTAAT TGGGGTCCAG 
AATCTGCTAA AGAAACATCA AGCCTTACAA GCAGAAATTG CTGGACATGA ACCACGCATC 
AAAGCAGTTA CACAGAAGGG GAATGCCATG GTGGAGGAAG GCCATTTTGC TGCAGAGGAT 
GTGAAGGCCA AGCTTCACGA GCTGAACCAA AAGTGGGAGG CACTGAAAGC CAAAGCTTCC 
CAGCGTCGGC AGGACCTGGA GGACTCTCTG CAGGCCCAGC AGTACTTTGC TGATGCTAAC 
GAGGCTGAAT CCTGGATGCG GGAGAAGGAA CCCATTGTGG GCAGCACTGA CTATGGCAAG 
GACGAAGACT CTGCTGAGGC TCTACTGAAG AAACACGAAG CTTTGATGTC AGATCTCAGT 
GCCTACGGCA GCAGCATCCA GGCTTTGCGA GAACAAGCAC AGTCCTGCCG GCAACAAGTG 
GCCCCCACGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CTCTCTACGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAG GGAGATATCC TTACCTTACT CAACAGCACC 
AACAAGGATT GGTGGAAAGT GGAAGTGAAC GATCGTCAGG GTTTTGTGCC GGCTGCGTAC 
GTGAAGAAAT TGGACCCCGC CCAGTCAGCC TCCCGGGAGA ATCTCCTGGA GGAGCAAGGC 
AGCATAGCAC TGCGGCAGGA GCAGATTGAC AATCAGACAC GCATAACTAA GGAGGCCGGC 
AGTGTATCTC TGCGTATGAA GCAGGTGGAA GAACTATATC ATTCTCTGCT GGAACTGGGT 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TTATGTTGTT CCGTGAAGCG 
AATGAACTAC AGCAATGGAT CAATGAGAAG GAAGCCGCTC TGACAAGTGA GGAGGTCGGA 
GCAGACTTGG AGCAGGTTGA GGTGCTCCAG AAGAAGTTTG ATGACTTCCA GAAGGACCTG 
AAGGCCAATG AGTCACGGTT GAAGGACATT AACAAGGTAG CTGAAGACCT GGAGTCTGAA 
GGTCTCATGG CAGAGGAGGT GCAGGCTGTG CAACAACAGG AAGTGTATGG CATGATGCCC 
AGGGATGAAA CTGATTCCAA GACAGCCTCC CCGTGGAAGT CTGCTCGTCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTACAGCAG 
CTGGCCGAGG AACGGAGCCA GCTCTTGGGC AGCGCCCATG AAGTACAGAG GTTCCACAGA 
GATGCTGATG AAACCAAAGA ATGGATTGAA GAGAAGAATC AAGCTCTAAA CACAGACAAT 
TATGGACATG ATCTCGCCAG TGTCCAGGCC CTGCAACGCA AGCATGAGGG CTTCGAGAGG 
GACCTTGCGG CTCTCGGTGA CAAGGTAAAC TCCCTTGGTG AAACAGCAGA GCGCCTGATC 
CAGTCCCATC CCGAGTCAGC AGAAGACCTG CAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGAGCAGCC TGGGGAAACG TGCAGATCAG CGCAAGGCAA AGTTGGGTGA CTCCCACGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGGGGG 
TTGGTGTCCT CAGATGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCATT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATCGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGACAGC AGCTGTTGGC TCACGGACAC TATGCCAGCC CTGAGATCAA GCAGAAACTT 
GATATTCTTG ACCAGGAGCG TGCAGACCTG GAGAAGGCCT GGGTTCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAACT GCAGCTGTTC CATCGGGACT GTGAGCAAGC TGAGAACTGG 
ATGGCTGCCC GGGAGGCCTT CTTGAATACC GAAGACAAAG GAGACTCACT GGACAGCGTA 
GAGGCTCTGA TCAAAAAACA TGAAGACTTT GACAAAGCGA TTAACGTCCA GGAAGAGAAG 
ATTGCTGCTC TGCAGGCCTT TGCCGACCAG CTCATCGCTG CCGGCCATTA TGCCAAGGGA 
GACATTTCTA GCCGGCGCAA TGAGGTCTTG GACAGGTGGC GACGTCTGAA AGCCCAGATG 
ATTGAGAAAA GGTCAAAGCT AGGAGAATCT CAAACCCTCC AACAGTTCAG CCGGGATGTG 
GATGAGATTG AGGCTTGGAT CAGTGAAAAA TTGCAAACAG CGAGTGATGA GTCGTACAAG 
GATCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ACCAGAAGCA CCAGGCTTTT 
GAAGCAGAGC TGCATGCCAA CGCTGACCGG ATCCGTGGGG TTATCGACAT GGGCAACTCC 
CTCATTGAAC GTGGAGCCTG TGCCGGCAGT GAGGATGCTG TCAAGGCCCG CCTGGCTGCC 
TTAGCTGACC AGTGGCAGTT CTTGGTGCAA AAGTCAGCGG AAAAGAGCCA GAAACTGAAA 
GAAGCCAACA AGCAGCAGAA CTTCAACACA GGGATCAAGG ACTTTGACTT CTGGCTGTCT 
GAGGTGGAGG CCCTGCTGGC ATCCGAAGAT TATGGCAAAG ACCTAGCTTC TGTGAACAAC 
CTGCTGAAAA AGCATCAACT GCTGGAAGCA GATATATCTG CCCATGAGGA TCGCCTGAAG 
GACCTGAACA GCCAGGCAGA CAGCCTGATG ACCAGCAGTG CCTTCGACAC CTCCCAAGTA 
AAGGACAAGA GGGACACCAT CAACGGGCGC TTCCAGAAGA TCAAGAGCAT GGCGGCCTCC 
CGGCGAGCCA AGCTGAATGA ATCCCATCGC CTGCACCAGT TCTTCCGGGA CATGGATGAC 
GAGGAGTCCT GGATCAAGGA GAAGAAGCTG CTGGTGGGCT CAGAGGACTA CGGCCGGGAC 
CTAACCGGCG TGCAGAACCT GAGGAAGAAG CACAAGCGGC TGGAAGCAGA ACTGGCTGCG 
CATGAGCCGG CTATTCAGGG TGTCCTGGAC ACTGGCAAGA AGCTGTCCGA TGACAACACC 
ATCGGGAAAG AGGAGATCCA GCAGCGGCTG GCGCAGTTTG TGGAGCACTG GAAAGAGCTG 
AAGCAGCTGG CAGCTGCCCG GGGTCAGCGG CTGGAAGAGT CCTTGGAATA TCAGCAGTTT 
GTAGCCAATG TGGAAGAGGA AGAAGCCTGG ATCAATGAGA AAATGACCCT GGTGGCCAGC 
GAAGATTATG GCGACACTCT TGCCGCCATC CAGGGCTTAC TGAAGAAACA TGAAGCTTTT 
GAGACAGACT TCACCGTCCA CAAGGATCGC GTGAATGATG TCTGCACCAA TGGACAAGAC 
CTCATTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CAAAGATGAA GGGCCTGAAC 
GGGAAAGTGT CAGACCTGGA GAAAGCTGCA GCCCAGAGAA AGGCGAAGCT GGATGAGAAC 
TCGGCCTTCC TTCAGTTCAA CTGGAAGGCG GACGTGGTGG AGTCCTGGAT CGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGC CGAGACCTGT CTTCTGTGCA GACGCTCCTC 
ACCAAACAGG AAACTTTTGA CGCTGGGCTG CAGGCCTTCC AGCAGGAAGG CATTGCCAAC 
ATCACTGCCC TCAAAGATCA GCTTCTCGCC GCCAAACACG TTCAGTCCAA GGCCATCGAG 
GCCCGGCACG CCTCCCTCAT GAAGAGGTGG AGCCAGCTTC TGGCCAACTC AGCCGCCCGC 
AAGAAGAAGC TTCTGGAGGC TCAGAGTCAC TTCCGCAAGG TGGAGGACCT CTTCCTGACC 
TTCGCCAAAA AGGCTTCTGC CTTCAACAGC TGGTTTGAAA ATGCAGAGGA GGACTTAACA 
GACCCCGTGC GCTGCAACTC CTTGGAAGAA ATCAAAGCTT TGCGCGAGGC CCACGACGCC 
TTCCGCTCCT CCCTCAGCTC TGCCCAGGCT GACTTCAACC AGCTGGCCGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT AGCCTCCAAC CCCTACACCT GGTTTACCAT GGAGGCCCTG 
GAGGAGACCT GGAGGAACCT ACAGAAAATC ATCAAGGAGA GGGAGCTGGA GCTGCAGAAG 
GAACAGCGGC GGCAGGAGGA GAACGACAAG CTGCGCCAGG AGTTTGCCCA GCACGCCAAC 
GCCTTCCACC AGTGGATCCA AGAGACCAGG ACATACCTCC TCGATGGGTC CTGTATGGTG 
GAAGAGTCGG GGACCCTCGA ATCCCAGCTT GAAGCTACCA AACGCAAGCA CCAGGAAATC 
CGAGCCATGA GAAGTCAGCT CAAAAAGATC GAGGACCTGG GGGCCGCCAT GGAGGAGGCC 
CTCATCCTGG ACAACAAGTA CACGGAGCAC AGCACCGTGG GCCTCGCCCA GCAGTGGGAC 
CAGCTGGACC AGCTGGGCAT GCGCATGCAG CACAACCTGG AGCAGCAGAT CCAGGCCAGG 
AACACAACAG GTGTGACTGA GGAGGCCCTC AAAGAATTCA GCATGATGTT TAAACACTTT 
GACAAGGACA AGTCTGGCAG GCTGAACCAT CAGGAGTTCA AATCTTGCCT GCGCTCCCTG 
GGCTATGACC TGCCCATGGT GGAGGAAGGG GAACCTGACC CTGAGTTCGA GGCAATCCTG 
GACACGGTGG ATCCGAACAG AGATGGCCAT GTCTCCTTGC AAGAATACAT GGCTTTCATG 
ATCAGCCGCG AAACTGAGAA CGTCAAGTCC AGCGAGGAGA TTGAGAGCGC CTTCCGGGCC 
CTCAGCTCAG AGGGAAAGCC TTACGTGACC AAGGAGGAGC TCTACCAGAA CCTGACCCGG 
GAACAAGCCG ACTACTGCGT CTCCCACATG AAGCCCTACG TGGACGGCAA GGGCCGCGAG 
CTCCCCACCG CGTTCGACTA CGTGGAGTTC ACCCGCTCGC TTTTCGTGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001123910.1
CDS	114..7547
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELASDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMRRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPTDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRITKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGMMPRDETDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKQKLDILDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAAGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINGRFQKIKSMAASRRAKLNESHRLHQFFRDMDDEESWIKEKKLLVGSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHASLMKRWSQLLANSAARKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	NM_001130438.3
Organism	Homo sapiens(human)
Definition	Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130438.3

ORF Insert Sequence:

ATGGACCCAA GTGGGGTCAA AGTGCTGGAA ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTAG ACCGATACCA CCGCTTCAAG GAACTCTCAA CCCTTAGGCG TCAGAAGCTG 
GAAGATTCCT ATCGATTCCA GTTCTTTCAA AGAGATGCTG AAGAGCTGGA GAAATGGATA 
CAGGAAAAAC TTCAGATTGC ATCTGATGAG AATTATAAAG ACCCAACCAA CTTGCAGGGA 
AAGCTTCAGA AGCATCAAGC ATTTGAAGCT GAAGTGCAGG CCAACTCAGG AGCCATTGTT 
AAGCTGGATG AAACTGGAAA CCTGATGATC TCAGAAGGGC ATTTTGCATC TGAAACCATA 
CGGACCCGTT TGATGGAGCT GCACCGCCAG TGGGAATTAC TTTTGGAGAA GATGCGAGAA 
AAAGGAATCA AACTGCTGCA GGCCCAGAAG TTGGTGCAGT ACTTACGAGA ATGTGAGGAC 
GTGATGGACT GGATCAATGA CAAGGAAGCA ATTGTTACTT CTGAAGAGCT GGGCCAGGAT 
CTGGAGCATG TAGAGGTTTT ACAGAAGAAA TTTGAAGAGT TTCAAACAGA TATGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTGAACCAG TTTGCTGCCA AACTCATACA GGAGCAGCAC 
CCTGAGGAGG AACTGATCAA GACTAAGCAG GATGAAGTCA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CTCTGCAGAG GCAGGGGAAG CTCTTTGGGG CAGCAGAAGT TCAGCGCTTT 
AACAGGGATG TGGATGAGAC TATCAGTTGG ATTAAGGAAA AGGAGCAGTT AATGGCCTCT 
GATGATTTTG GCCGAGACCT GGCAAGTGTT CAGGCTCTGC TTCGGAAGCA CGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAAGACAAG GTCAAAGCCC TGTGTGCTGA GGCTGACCGC 
CTGCAACAGT CCCACCCTCT GAGTGCAACA CAGATTCAAG TGAAGCGAGA GGAACTGATT 
ACAAACTGGG AGCAGATCCG CACCTTGGCG GCAGAGAGAC ATGCACGGCT CAATGATTCA 
TACAGGCTTC AACGCTTCCT TGCTGACTTC CGTGACCTCA CCAGCTGGGT GACTGAGATG 
AAAGCCCTCA TCAATGCAGA TGAGCTTGCC AGTGATGTGG CTGGGGCTGA AGCCCTGCTA 
GATAGACACC AAGAGCACAA GGGTGAAATT GATGCCCATG AAGACAGCTT CAAATCTGCA 
GATGAATCTG GACAGGCACT GCTTGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGACCG TCCTTTCCGA GGAGAGAGCG GCGCTGCTGG AGCTGTGGGA GCTGCGCAGG 
CAGCAGTACG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GGGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAATGAAG ACTTGGGAGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTTAA GAAGCACGAA GACTTTGAGA AATCCCTTAG TGCCCAGGAG 
GAAAAGATTA CAGCATTAGA TGAATTTGCA ACCAAGCTAA TTCAGAACAA CCACTATGCA 
ATGGAAGATG TGGCCACTCG CCGAGATGCT CTGTTGAGCC GCCGCAATGC CCTTCACGAG 
AGAGCCATGC GTCGCCGGGC CCAGCTAGCC GATTCTTTCC ATCTGCAGCA GTTTTTCCGT 
GATTCTGATG AGCTCAAGAG TTGGGTCAAT GAGAAGATGA AAACTGCCAC AGATGAAGCT 
TATAAAGATC CATCCAACCT ACAAGGAAAA GTACAGAAGC ATCAGGCTTT TGAGGCTGAG 
CTCTCAGCAA ACCAGAGCCG AATTGATGCC TTGGAGAAAG CTGGCCAAAA GCTGATTGAT 
GTCAACCACT ATGCCAAGGA TGAAGTGGCA GCTCGTATGA ATGAGGTGAT CAGTTTGTGG 
AAGAAACTGC TAGAGGCCAC TGAACTGAAA GGAATAAAGC TTCGTGAAGC CAACCAGCAA 
CAGCAATTTA ATCGCAATGT TGAGGATATT GAATTGTGGC TATATGAAGT AGAAGGTCAC 
TTGGCTTCGG ATGATTACGG CAAAGATCTT ACCAATGTGC AGAACCTCCA GAAGAAACAT 
GCACTGCTAG AGGCAGATGT GGCTGCTCAC CAGGACCGAA TTGATGGCAT CACCATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTT GATGCAGAAA ACATCAAGAA GAAACAGGAA 
GCCCTCGTGG CTCGCTATGA GGCACTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCCGATTCTC TGCGGTTGCA GCAGCTCTTC CGGGATGTTG AGGATGAGGA GACGTGGATT 
CGAGAGAAAG AGCCCATTGC CGCATCTACC AACAGAGGTA AGGATTTAAT TGGGGTCCAG 
AATCTGCTAA AGAAACATCA AGCCTTACAA GCAGAAATTG CTGGACATGA ACCACGCATC 
AAAGCAGTTA CACAGAAGGG GAATGCCATG GTGGAGGAAG GCCATTTTGC TGCAGAGGAT 
GTGAAGGCCA AGCTTCACGA GCTGAACCAA AAGTGGGAGG CACTGAAAGC CAAAGCTTCC 
CAGCGTCGGC AGGACCTGGA GGACTCTCTG CAGGCCCAGC AGTACTTTGC TGATGCTAAC 
GAGGCTGAAT CCTGGATGCG GGAGAAGGAA CCCATTGTGG GCAGCACTGA CTATGGCAAG 
GACGAAGACT CTGCTGAGGC TCTACTGAAG AAACACGAAG CTTTGATGTC AGATCTCAGT 
GCCTACGGCA GCAGCATCCA GGCTTTGCGA GAACAAGCAC AGTCCTGCCG GCAACAAGTG 
GCCCCCACGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CTCTCTACGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAG GGAGATATCC TTACCTTACT CAACAGCACC 
AACAAGGATT GGTGGAAAGT GGAAGTGAAC GATCGTCAGG GTTTTGTGCC GGCTGCGTAC 
GTGAAGAAAT TGGACCCCGC CCAGTCAGCC TCCCGGGAGA ATCTCCTGGA GGAGCAAGGC 
AGCATAGCAC TGCGGCAGGA GCAGATTGAC AATCAGACAC GCATAACTAA GGAGGCCGGC 
AGTGTATCTC TGCGTATGAA GCAGGTGGAA GAACTATATC ATTCTCTGCT GGAACTGGGT 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TTATGTTGTT CCGTGAAGCG 
AATGAACTAC AGCAATGGAT CAATGAGAAG GAAGCCGCTC TGACAAGTGA GGAGGTCGGA 
GCAGACTTGG AGCAGGTTGA GGTGCTCCAG AAGAAGTTTG ATGACTTCCA GAAGGACCTG 
AAGGCCAATG AGTCACGGTT GAAGGACATT AACAAGGTAG CTGAAGACCT GGAGTCTGAA 
GGTCTCATGG CAGAGGAGGT GCAGGCTGTG CAACAACAGG AAGTGTATGG CATGATGCCC 
AGGGATGAAA CTGATTCCAA GACAGCCTCC CCGTGGAAGT CTGCTCGTCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTACAGCAG 
CTGGCCGAGG AACGGAGCCA GCTCTTGGGC AGCGCCCATG AAGTACAGAG GTTCCACAGA 
GATGCTGATG AAACCAAAGA ATGGATTGAA GAGAAGAATC AAGCTCTAAA CACAGACAAT 
TATGGACATG ATCTCGCCAG TGTCCAGGCC CTGCAACGCA AGCATGAGGG CTTCGAGAGG 
GACCTTGCGG CTCTCGGTGA CAAGGTAAAC TCCCTTGGTG AAACAGCAGA GCGCCTGATC 
CAGTCCCATC CCGAGTCAGC AGAAGACCTG CAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGAGCAGCC TGGGGAAACG TGCAGATCAG CGCAAGGCAA AGTTGGGTGA CTCCCACGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGGGGG 
TTGGTGTCCT CAGATGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCATT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATCGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGACAGC AGCTGTTGGC TCACGGACAC TATGCCAGCC CTGAGATCAA GCAGAAACTT 
GATATTCTTG ACCAGGAGCG TGCAGACCTG GAGAAGGCCT GGGTTCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAACT GCAGCTGTTC CATCGGGACT GTGAGCAAGC TGAGAACTGG 
ATGGCTGCCC GGGAGGCCTT CTTGAATACC GAAGACAAAG GAGACTCACT GGACAGCGTA 
GAGGCTCTGA TCAAAAAACA TGAAGACTTT GACAAAGCGA TTAACGTCCA GGAAGAGAAG 
ATTGCTGCTC TGCAGGCCTT TGCCGACCAG CTCATCGCTG CCGGCCATTA TGCCAAGGGA 
GACATTTCTA GCCGGCGCAA TGAGGTCTTG GACAGGTGGC GACGTCTGAA AGCCCAGATG 
ATTGAGAAAA GGTCAAAGCT AGGAGAATCT CAAACCCTCC AACAGTTCAG CCGGGATGTG 
GATGAGATTG AGGCTTGGAT CAGTGAAAAA TTGCAAACAG CGAGTGATGA GTCGTACAAG 
GATCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ACCAGAAGCA CCAGGCTTTT 
GAAGCAGAGC TGCATGCCAA CGCTGACCGG ATCCGTGGGG TTATCGACAT GGGCAACTCC 
CTCATTGAAC GTGGAGCCTG TGCCGGCAGT GAGGATGCTG TCAAGGCCCG CCTGGCTGCC 
TTAGCTGACC AGTGGCAGTT CTTGGTGCAA AAGTCAGCGG AAAAGAGCCA GAAACTGAAA 
GAAGCCAACA AGCAGCAGAA CTTCAACACA GGGATCAAGG ACTTTGACTT CTGGCTGTCT 
GAGGTGGAGG CCCTGCTGGC ATCCGAAGAT TATGGCAAAG ACCTAGCTTC TGTGAACAAC 
CTGCTGAAAA AGCATCAACT GCTGGAAGCA GATATATCTG CCCATGAGGA TCGCCTGAAG 
GACCTGAACA GCCAGGCAGA CAGCCTGATG ACCAGCAGTG CCTTCGACAC CTCCCAAGTA 
AAGGACAAGA GGGACACCAT CAACGGGCGC TTCCAGAAGA TCAAGAGCAT GGCGGCCTCC 
CGGCGAGCCA AGCTGAATGA ATCCCATCGC CTGCACCAGT TCTTCCGGGA CATGGATGAC 
GAGGAGTCCT GGATCAAGGA GAAGAAGCTG CTGGTGGGCT CAGAGGACTA CGGCCGGGAC 
CTAACCGGCG TGCAGAACCT GAGGAAGAAG CACAAGCGGC TGGAAGCAGA ACTGGCTGCG 
CATGAGCCGG CTATTCAGGG TGTCCTGGAC ACTGGCAAGA AGCTGTCCGA TGACAACACC 
ATCGGGAAAG AGGAGATCCA GCAGCGGCTG GCGCAGTTTG TGGAGCACTG GAAAGAGCTG 
AAGCAGCTGG CAGCTGCCCG GGGTCAGCGG CTGGAAGAGT CCTTGGAATA TCAGCAGTTT 
GTAGCCAATG TGGAAGAGGA AGAAGCCTGG ATCAATGAGA AAATGACCCT GGTGGCCAGC 
GAAGATTATG GCGACACTCT TGCCGCCATC CAGGGCTTAC TGAAGAAACA TGAAGCTTTT 
GAGACAGACT TCACCGTCCA CAAGGATCGC GTGAATGATG TCTGCACCAA TGGACAAGAC 
CTCATTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CAAAGATGAA GGGCCTGAAC 
GGGAAAGTGT CAGACCTGGA GAAAGCTGCA GCCCAGAGAA AGGCGAAGCT GGATGAGAAC 
TCGGCCTTCC TTCAGTTCAA CTGGAAGGCG GACGTGGTGG AGTCCTGGAT CGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGC CGAGACCTGT CTTCTGTGCA GACGCTCCTC 
ACCAAACAGG AAACTTTTGA CGCTGGGCTG CAGGCCTTCC AGCAGGAAGG CATTGCCAAC 
ATCACTGCCC TCAAAGATCA GCTTCTCGCC GCCAAACACG TTCAGTCCAA GGCCATCGAG 
GCCCGGCACG CCTCCCTCAT GAAGAGGTGG AGCCAGCTTC TGGCCAACTC AGCCGCCCGC 
AAGAAGAAGC TTCTGGAGGC TCAGAGTCAC TTCCGCAAGG TGGAGGACCT CTTCCTGACC 
TTCGCCAAAA AGGCTTCTGC CTTCAACAGC TGGTTTGAAA ATGCAGAGGA GGACTTAACA 
GACCCCGTGC GCTGCAACTC CTTGGAAGAA ATCAAAGCTT TGCGCGAGGC CCACGACGCC 
TTCCGCTCCT CCCTCAGCTC TGCCCAGGCT GACTTCAACC AGCTGGCCGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT AGCCTCCAAC CCCTACACCT GGTTTACCAT GGAGGCCCTG 
GAGGAGACCT GGAGGAACCT ACAGAAAATC ATCAAGGAGA GGGAGCTGGA GCTGCAGAAG 
GAACAGCGGC GGCAGGAGGA GAACGACAAG CTGCGCCAGG AGTTTGCCCA GCACGCCAAC 
GCCTTCCACC AGTGGATCCA AGAGACCAGG ACATACCTCC TCGATGGGTC CTGTATGGTG 
GAAGAGTCGG GGACCCTCGA ATCCCAGCTT GAAGCTACCA AACGCAAGCA CCAGGAAATC 
CGAGCCATGA GAAGTCAGCT CAAAAAGATC GAGGACCTGG GGGCCGCCAT GGAGGAGGCC 
CTCATCCTGG ACAACAAGTA CACGGAGCAC AGCACCGTGG GCCTCGCCCA GCAGTGGGAC 
CAGCTGGACC AGCTGGGCAT GCGCATGCAG CACAACCTGG AGCAGCAGAT CCAGGCCAGG 
AACACAACAG GTGTGACTGA GGAGGCCCTC AAAGAATTCA GCATGATGTT TAAACACTTT 
GACAAGGACA AGTCTGGCAG GCTGAACCAT CAGGAGTTCA AATCTTGCCT GCGCTCCCTG 
GGCTATGACC TGCCCATGGT GGAGGAAGGG GAACCTGACC CTGAGTTCGA GGCAATCCTG 
GACACGGTGG ATCCGAACAG AGATGGCCAT GTCTCCTTGC AAGAATACAT GGCTTTCATG 
ATCAGCCGCG AAACTGAGAA CGTCAAGTCC AGCGAGGAGA TTGAGAGCGC CTTCCGGGCC 
CTCAGCTCAG AGGGAAAGCC TTACGTGACC AAGGAGGAGC TCTACCAGAA CCTGACCCGG 
GAACAAGCCG ACTACTGCGT CTCCCACATG AAGCCCTACG TGGACGGCAA GGGCCGCGAG 
CTCCCCACCG CGTTCGACTA CGTGGAGTTC ACCCGCTCGC TTTTCGTGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( NM_001375310.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001362239.1 Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant 6, mRNA.

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CloneID	OHu109488
Clone ID Related Accession (Same CDS sequence)	NM_001375310.1
Accession Version	NM_001375310.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7521bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	spectrin alpha chain, non-erythrocytic 1 isoform 6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL356481.16. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.15450.1 [ECO:0000332] ##Evidence-Data-END##

ATGGACCCAA GTGGGGTCAA AGTGCTGGAA ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTAG ACCGATACCA CCGCTTCAAG GAACTCTCAA CCCTTAGGCG TCAGAAGCTG 
GAAGATTCCT ATCGATTCCA GTTCTTTCAA AGAGATGCTG AAGAGCTGGA GAAATGGATA 
CAGGAAAAAC TTCAGATTGC ATCTGATGAG AATTATAAAG ACCCAACCAA CTTGCAGGGA 
AAGCTTCAGA AGCATCAAGC ATTTGAAGCT GAAGTGCAGG CCAACTCAGG AGCCATTGTT 
AAGCTGGATG AAACTGGAAA CCTGATGATC TCAGAAGGGC ATTTTGCATC TGAAACCATA 
CGGACCCGTT TGATGGAGCT GCACCGCCAG TGGGAATTAC TTTTGGAGAA GATGCGAGAA 
AAAGGAATCA AACTGCTGCA GGCCCAGAAG TTGGTGCAGT ACTTACGAGA ATGTGAGGAC 
GTGATGGACT GGATCAATGA CAAGGAAGCA ATTGTTACTT CTGAAGAGCT GGGCCAGGAT 
CTGGAGCATG TAGAGGTTTT ACAGAAGAAA TTTGAAGAGT TTCAAACAGA TATGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTGAACCAG TTTGCTGCCA AACTCATACA GGAGCAGCAC 
CCTGAGGAGG AACTGATCAA GACTAAGCAG GATGAAGTCA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CTCTGCAGAG GCAGGGGAAG CTCTTTGGGG CAGCAGAAGT TCAGCGCTTT 
AACAGGGATG TGGATGAGAC TATCAGTTGG ATTAAGGAAA AGGAGCAGTT AATGGCCTCT 
GATGATTTTG GCCGAGACCT GGCAAGTGTT CAGGCTCTGC TTCGGAAGCA CGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAAGACAAG GTCAAAGCCC TGTGTGCTGA GGCTGACCGC 
CTGCAACAGT CCCACCCTCT GAGTGCAACA CAGATTCAAG TGAAGCGAGA GGAACTGATT 
ACAAACTGGG AGCAGATCCG CACCTTGGCG GCAGAGAGAC ATGCACGGCT CAATGATTCA 
TACAGGCTTC AACGCTTCCT TGCTGACTTC CGTGACCTCA CCAGCTGGGT GACTGAGATG 
AAAGCCCTCA TCAATGCAGA TGAGCTTGCC AGTGATGTGG CTGGGGCTGA AGCCCTGCTA 
GATAGACACC AAGAGCACAA GGGTGAAATT GATGCCCATG AAGACAGCTT CAAATCTGCA 
GATGAATCTG GACAGGCACT GCTTGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGACCG TCCTTTCCGA GGAGAGAGCG GCGCTGCTGG AGCTGTGGGA GCTGCGCAGG 
CAGCAGTACG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GGGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAATGAAG ACTTGGGAGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTTAA GAAGCACGAA GACTTTGAGA AATCCCTTAG TGCCCAGGAG 
GAAAAGATTA CAGCATTAGA TGAATTTGCA ACCAAGCTAA TTCAGAACAA CCACTATGCA 
ATGGAAGATG TGGCCACTCG CCGAGATGCT CTGTTGAGCC GCCGCAATGC CCTTCACGAG 
AGAGCCATGC GTCGCCGGGC CCAGCTAGCC GATTCTTTCC ATCTGCAGCA GTTTTTCCGT 
GATTCTGATG AGCTCAAGAG TTGGGTCAAT GAGAAGATGA AAACTGCCAC AGATGAAGCT 
TATAAAGATC CATCCAACCT ACAAGGAAAA GTACAGAAGC ATCAGGCTTT TGAGGCTGAG 
CTCTCAGCAA ACCAGAGCCG AATTGATGCC TTGGAGAAAG CTGGCCAAAA GCTGATTGAT 
GTCAACCACT ATGCCAAGGA TGAAGTGGCA GCTCGTATGA ATGAGGTGAT CAGTTTGTGG 
AAGAAACTGC TAGAGGCCAC TGAACTGAAA GGAATAAAGC TTCGTGAAGC CAACCAGCAA 
CAGCAATTTA ATCGCAATGT TGAGGATATT GAATTGTGGC TATATGAAGT AGAAGGTCAC 
TTGGCTTCGG ATGATTACGG CAAAGATCTT ACCAATGTGC AGAACCTCCA GAAGAAACAT 
GCACTGCTAG AGGCAGATGT GGCTGCTCAC CAGGACCGAA TTGATGGCAT CACCATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTT GATGCAGAAA ACATCAAGAA GAAACAGGAA 
GCCCTCGTGG CTCGCTATGA GGCACTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCCGATTCTC TGCGGTTGCA GCAGCTCTTC CGGGATGTTG AGGATGAGGA GACGTGGATT 
CGAGAGAAAG AGCCCATTGC CGCATCTACC AACAGAGGTA AGGATTTAAT TGGGGTCCAG 
AATCTGCTAA AGAAACATCA AGCCTTACAA GCAGAAATTG CTGGACATGA ACCACGCATC 
AAAGCAGTTA CACAGAAGGG GAATGCCATG GTGGAGGAAG GCCATTTTGC TGCAGAGGAT 
GTGAAGGCCA AGCTTCACGA GCTGAACCAA AAGTGGGAGG CACTGAAAGC CAAAGCTTCC 
CAGCGTCGGC AGGACCTGGA GGACTCTCTG CAGGCCCAGC AGTACTTTGC TGATGCTAAC 
GAGGCTGAAT CCTGGATGCG GGAGAAGGAA CCCATTGTGG GCAGCACTGA CTATGGCAAG 
GACGAAGACT CTGCTGAGGC TCTACTGAAG AAACACGAAG CTTTGATGTC AGATCTCAGT 
GCCTACGGCA GCAGCATCCA GGCTTTGCGA GAACAAGCAC AGTCCTGCCG GCAACAAGTG 
GCCCCCACGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CTCTCTACGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAG GGAGATATCC TTACCTTACT CAACAGCACC 
AACAAGGATT GGTGGAAAGT GGAAGTGAAC GATCGTCAGG GTTTTGTGCC GGCTGCGTAC 
GTGAAGAAAT TGGACCCCGC CCAGTCAGCC TCCCGGGAGA ATCTCCTGGA GGAGCAAGGC 
AGCATAGCAC TGCGGCAGGA GCAGATTGAC AATCAGACAC GCATAACTAA GGAGGCCGGC 
AGTGTATCTC TGCGTATGAA GCAGGTGGAA GAACTATATC ATTCTCTGCT GGAACTGGGT 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TTATGTTGTT CCGTGAAGCG 
AATGAACTAC AGCAATGGAT CAATGAGAAG GAAGCCGCTC TGACAAGTGA GGAGGTCGGA 
GCAGACTTGG AGCAGGTTGA GGTGCTCCAG AAGAAGTTTG ATGACTTCCA GAAGGACCTG 
AAGGCCAATG AGTCACGGTT GAAGGACATT AACAAGGTAG CTGAAGACCT GGAGTCTGAA 
GGTCTCATGG CAGAGGAGGT GCAGGCTGTG CAACAACAGG AAGTGTATGG CATGATGCCC 
AGGGATGAAA CTGATTCCAA GACAGCCTCC CCGTGGAAGT CTGCTCGTCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTACAGCAG 
CTGGCCGAGG AACGGAGCCA GCTCTTGGGC AGCGCCCATG AAGTACAGAG GTTCCACAGA 
GATGCTGATG AAACCAAAGA ATGGATTGAA GAGAAGAATC AAGCTCTAAA CACAGACAAT 
TATGGACATG ATCTCGCCAG TGTCCAGGCC CTGCAACGCA AGCATGAGGG CTTCGAGAGG 
GACCTTGCGG CTCTCGGTGA CAAGGTAAAC TCCCTTGGTG AAACAGCAGA GCGCCTGATC 
CAGTCCCATC CCGAGTCAGC AGAAGACCTG CAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGAGCAGCC TGGGGAAACG TGCAGATCAG CGCAAGGCAA AGTTGGGTGA CTCCCACGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGGGGG 
TTGGTGTCCT CAGATGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCATT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATCGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGACAGC AGCTGTTGGC TCACGGACAC TATGCCAGCC CTGAGATCAA GCAGAAACTT 
GATATTCTTG ACCAGGAGCG TGCAGACCTG GAGAAGGCCT GGGTTCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAACT GCAGCTGTTC CATCGGGACT GTGAGCAAGC TGAGAACTGG 
ATGGCTGCCC GGGAGGCCTT CTTGAATACC GAAGACAAAG GAGACTCACT GGACAGCGTA 
GAGGCTCTGA TCAAAAAACA TGAAGACTTT GACAAAGCGA TTAACGTCCA GGAAGAGAAG 
ATTGCTGCTC TGCAGGCCTT TGCCGACCAG CTCATCGCTG CCGGCCATTA TGCCAAGGGA 
GACATTTCTA GCCGGCGCAA TGAGGTCTTG GACAGGTGGC GACGTCTGAA AGCCCAGATG 
ATTGAGAAAA GGTCAAAGCT AGGAGAATCT CAAACCCTCC AACAGTTCAG CCGGGATGTG 
GATGAGATTG AGGCTTGGAT CAGTGAAAAA TTGCAAACAG CGAGTGATGA GTCGTACAAG 
GATCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ACCAGAAGCA CCAGGCTTTT 
GAAGCAGAGC TGCATGCCAA CGCTGACCGG ATCCGTGGGG TTATCGACAT GGGCAACTCC 
CTCATTGAAC GTGGAGCCTG TGCCGGCAGT GAGGATGCTG TCAAGGCCCG CCTGGCTGCC 
TTAGCTGACC AGTGGCAGTT CTTGGTGCAA AAGTCAGCGG AAAAGAGCCA GAAACTGAAA 
GAAGCCAACA AGCAGCAGAA CTTCAACACA GGGATCAAGG ACTTTGACTT CTGGCTGTCT 
GAGGTGGAGG CCCTGCTGGC ATCCGAAGAT TATGGCAAAG ACCTAGCTTC TGTGAACAAC 
CTGCTGAAAA AGCATCAACT GCTGGAAGCA GATATATCTG CCCATGAGGA TCGCCTGAAG 
GACCTGAACA GCCAGGCAGA CAGCCTGATG ACCAGCAGTG CCTTCGACAC CTCCCAAGTA 
AAGGACAAGA GGGACACCAT CAACGGGCGC TTCCAGAAGA TCAAGAGCAT GGCGGCCTCC 
CGGCGAGCCA AGCTGAATGA ATCCCATCGC CTGCACCAGT TCTTCCGGGA CATGGATGAC 
GAGGAGTCCT GGATCAAGGA GAAGAAGCTG CTGGTGGGCT CAGAGGACTA CGGCCGGGAC 
CTAACCGGCG TGCAGAACCT GAGGAAGAAG CACAAGCGGC TGGAAGCAGA ACTGGCTGCG 
CATGAGCCGG CTATTCAGGG TGTCCTGGAC ACTGGCAAGA AGCTGTCCGA TGACAACACC 
ATCGGGAAAG AGGAGATCCA GCAGCGGCTG GCGCAGTTTG TGGAGCACTG GAAAGAGCTG 
AAGCAGCTGG CAGCTGCCCG GGGTCAGCGG CTGGAAGAGT CCTTGGAATA TCAGCAGTTT 
GTAGCCAATG TGGAAGAGGA AGAAGCCTGG ATCAATGAGA AAATGACCCT GGTGGCCAGC 
GAAGATTATG GCGACACTCT TGCCGCCATC CAGGGCTTAC TGAAGAAACA TGAAGCTTTT 
GAGACAGACT TCACCGTCCA CAAGGATCGC GTGAATGATG TCTGCACCAA TGGACAAGAC 
CTCATTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CAAAGATGAA GGGCCTGAAC 
GGGAAAGTGT CAGACCTGGA GAAAGCTGCA GCCCAGAGAA AGGCGAAGCT GGATGAGAAC 
TCGGCCTTCC TTCAGTTCAA CTGGAAGGCG GACGTGGTGG AGTCCTGGAT CGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGC CGAGACCTGT CTTCTGTGCA GACGCTCCTC 
ACCAAACAGG AAACTTTTGA CGCTGGGCTG CAGGCCTTCC AGCAGGAAGG CATTGCCAAC 
ATCACTGCCC TCAAAGATCA GCTTCTCGCC GCCAAACACG TTCAGTCCAA GGCCATCGAG 
GCCCGGCACG CCTCCCTCAT GAAGAGGTGG AGCCAGCTTC TGGCCAACTC AGCCGCCCGC 
AAGAAGAAGC TTCTGGAGGC TCAGAGTCAC TTCCGCAAGG TGGAGGACCT CTTCCTGACC 
TTCGCCAAAA AGGCTTCTGC CTTCAACAGC TGGTTTGAAA ATGCAGAGGA GGACTTAACA 
GACCCCGTGC GCTGCAACTC CTTGGAAGAA ATCAAAGCTT TGCGCGAGGC CCACGACGCC 
TTCCGCTCCT CCCTCAGCTC TGCCCAGGCT GACTTCAACC AGCTGGCCGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT AGCCTCCAAC CCCTACACCT GGTTTACCAT GGAGGCCCTG 
GAGGAGACCT GGAGGAACCT ACAGAAAATC ATCAAGGGTG TTGCTATGTT GCCCAGGCTG 
GTCTTCAACT CCTGGCCTCA AGCAGTCCTC CTGCCCAGGC CTCCCAAAGT GCTGGGATTA 
CAGGAGAGGG AGCTGGAGCT GCAGAAGGAA CAGCGGCGGC AGGAGGAGAA CGACAAGCTG 
CGCCAGGAGT TTGCCCAGCA CGCCAACGCC TTCCACCAGT GGATCCAAGA GACCAGGACA 
TACCTCCTCG ATGGGTCCTG TATGGTGGAA GAGTCGGGGA CCCTCGAATC CCAGCTTGAA 
GCTACCAAAC GCAAGCACCA GGAAATCCGA GCCATGAGAA GTCAGCTCAA AAAGATCGAG 
GACCTGGGGG CCGCCATGGA GGAGGCCCTC ATCCTGGACA ACAAGTACAC GGAGCACAGC 
ACCGTGGGCC TCGCCCAGCA GTGGGACCAG CTGGACCAGC TGGGCATGCG CATGCAGCAC 
AACCTGGAGC AGCAGATCCA GGCCAGGAAC ACAACAGGTG TGACTGAGGA GGCCCTCAAA 
GAATTCAGCA TGATGTTTAA ACACTTTGAC AAGGACAAGT CTGGCAGGCT GAACCATCAG 
GAGTTCAAAT CTTGCCTGCG CTCCCTGGGC TATGACCTGC CCATGGTGGA GGAAGGGGAA 
CCTGACCCTG AGTTCGAGGC AATCCTGGAC ACGGTGGATC CGAACAGAGA TGGCCATGTC 
TCCTTGCAAG AATACATGGC TTTCATGATC AGCCGCGAAA CTGAGAACGT CAAGTCCAGC 
GAGGAGATTG AGAGCGCCTT CCGGGCCCTC AGCTCAGAGG GAAAGCCTTA CGTGACCAAG 
GAGGAGCTCT ACCAGAACCT GACCCGGGAA CAAGCCGACT ACTGCGTCTC CCACATGAAG 
CCCTACGTGG ACGGCAAGGG CCGCGAGCTC CCCACCGCGT TCGACTACGT GGAGTTCACC 
CGCTCGCTTT TCGTGAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001362239.1
CDS	114..7634
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELASDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMRRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPTDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRITKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGMMPRDETDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKQKLDILDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAAGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINGRFQKIKSMAASRRAKLNESHRLHQFFRDMDDEESWIKEKKLLVGSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHASLMKRWSQLLANSAARKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKGVAMLPRLVFNSWPQAVLLPRPPKVLGLQERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	NM_001375310.1
Organism	Homo sapiens(human)
Definition	Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001375310.1

ORF Insert Sequence:

ATGGACCCAA GTGGGGTCAA AGTGCTGGAA ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTAG ACCGATACCA CCGCTTCAAG GAACTCTCAA CCCTTAGGCG TCAGAAGCTG 
GAAGATTCCT ATCGATTCCA GTTCTTTCAA AGAGATGCTG AAGAGCTGGA GAAATGGATA 
CAGGAAAAAC TTCAGATTGC ATCTGATGAG AATTATAAAG ACCCAACCAA CTTGCAGGGA 
AAGCTTCAGA AGCATCAAGC ATTTGAAGCT GAAGTGCAGG CCAACTCAGG AGCCATTGTT 
AAGCTGGATG AAACTGGAAA CCTGATGATC TCAGAAGGGC ATTTTGCATC TGAAACCATA 
CGGACCCGTT TGATGGAGCT GCACCGCCAG TGGGAATTAC TTTTGGAGAA GATGCGAGAA 
AAAGGAATCA AACTGCTGCA GGCCCAGAAG TTGGTGCAGT ACTTACGAGA ATGTGAGGAC 
GTGATGGACT GGATCAATGA CAAGGAAGCA ATTGTTACTT CTGAAGAGCT GGGCCAGGAT 
CTGGAGCATG TAGAGGTTTT ACAGAAGAAA TTTGAAGAGT TTCAAACAGA TATGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTGAACCAG TTTGCTGCCA AACTCATACA GGAGCAGCAC 
CCTGAGGAGG AACTGATCAA GACTAAGCAG GATGAAGTCA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CTCTGCAGAG GCAGGGGAAG CTCTTTGGGG CAGCAGAAGT TCAGCGCTTT 
AACAGGGATG TGGATGAGAC TATCAGTTGG ATTAAGGAAA AGGAGCAGTT AATGGCCTCT 
GATGATTTTG GCCGAGACCT GGCAAGTGTT CAGGCTCTGC TTCGGAAGCA CGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAAGACAAG GTCAAAGCCC TGTGTGCTGA GGCTGACCGC 
CTGCAACAGT CCCACCCTCT GAGTGCAACA CAGATTCAAG TGAAGCGAGA GGAACTGATT 
ACAAACTGGG AGCAGATCCG CACCTTGGCG GCAGAGAGAC ATGCACGGCT CAATGATTCA 
TACAGGCTTC AACGCTTCCT TGCTGACTTC CGTGACCTCA CCAGCTGGGT GACTGAGATG 
AAAGCCCTCA TCAATGCAGA TGAGCTTGCC AGTGATGTGG CTGGGGCTGA AGCCCTGCTA 
GATAGACACC AAGAGCACAA GGGTGAAATT GATGCCCATG AAGACAGCTT CAAATCTGCA 
GATGAATCTG GACAGGCACT GCTTGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGACCG TCCTTTCCGA GGAGAGAGCG GCGCTGCTGG AGCTGTGGGA GCTGCGCAGG 
CAGCAGTACG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GGGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAATGAAG ACTTGGGAGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTTAA GAAGCACGAA GACTTTGAGA AATCCCTTAG TGCCCAGGAG 
GAAAAGATTA CAGCATTAGA TGAATTTGCA ACCAAGCTAA TTCAGAACAA CCACTATGCA 
ATGGAAGATG TGGCCACTCG CCGAGATGCT CTGTTGAGCC GCCGCAATGC CCTTCACGAG 
AGAGCCATGC GTCGCCGGGC CCAGCTAGCC GATTCTTTCC ATCTGCAGCA GTTTTTCCGT 
GATTCTGATG AGCTCAAGAG TTGGGTCAAT GAGAAGATGA AAACTGCCAC AGATGAAGCT 
TATAAAGATC CATCCAACCT ACAAGGAAAA GTACAGAAGC ATCAGGCTTT TGAGGCTGAG 
CTCTCAGCAA ACCAGAGCCG AATTGATGCC TTGGAGAAAG CTGGCCAAAA GCTGATTGAT 
GTCAACCACT ATGCCAAGGA TGAAGTGGCA GCTCGTATGA ATGAGGTGAT CAGTTTGTGG 
AAGAAACTGC TAGAGGCCAC TGAACTGAAA GGAATAAAGC TTCGTGAAGC CAACCAGCAA 
CAGCAATTTA ATCGCAATGT TGAGGATATT GAATTGTGGC TATATGAAGT AGAAGGTCAC 
TTGGCTTCGG ATGATTACGG CAAAGATCTT ACCAATGTGC AGAACCTCCA GAAGAAACAT 
GCACTGCTAG AGGCAGATGT GGCTGCTCAC CAGGACCGAA TTGATGGCAT CACCATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTT GATGCAGAAA ACATCAAGAA GAAACAGGAA 
GCCCTCGTGG CTCGCTATGA GGCACTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCCGATTCTC TGCGGTTGCA GCAGCTCTTC CGGGATGTTG AGGATGAGGA GACGTGGATT 
CGAGAGAAAG AGCCCATTGC CGCATCTACC AACAGAGGTA AGGATTTAAT TGGGGTCCAG 
AATCTGCTAA AGAAACATCA AGCCTTACAA GCAGAAATTG CTGGACATGA ACCACGCATC 
AAAGCAGTTA CACAGAAGGG GAATGCCATG GTGGAGGAAG GCCATTTTGC TGCAGAGGAT 
GTGAAGGCCA AGCTTCACGA GCTGAACCAA AAGTGGGAGG CACTGAAAGC CAAAGCTTCC 
CAGCGTCGGC AGGACCTGGA GGACTCTCTG CAGGCCCAGC AGTACTTTGC TGATGCTAAC 
GAGGCTGAAT CCTGGATGCG GGAGAAGGAA CCCATTGTGG GCAGCACTGA CTATGGCAAG 
GACGAAGACT CTGCTGAGGC TCTACTGAAG AAACACGAAG CTTTGATGTC AGATCTCAGT 
GCCTACGGCA GCAGCATCCA GGCTTTGCGA GAACAAGCAC AGTCCTGCCG GCAACAAGTG 
GCCCCCACGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CTCTCTACGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAG GGAGATATCC TTACCTTACT CAACAGCACC 
AACAAGGATT GGTGGAAAGT GGAAGTGAAC GATCGTCAGG GTTTTGTGCC GGCTGCGTAC 
GTGAAGAAAT TGGACCCCGC CCAGTCAGCC TCCCGGGAGA ATCTCCTGGA GGAGCAAGGC 
AGCATAGCAC TGCGGCAGGA GCAGATTGAC AATCAGACAC GCATAACTAA GGAGGCCGGC 
AGTGTATCTC TGCGTATGAA GCAGGTGGAA GAACTATATC ATTCTCTGCT GGAACTGGGT 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TTATGTTGTT CCGTGAAGCG 
AATGAACTAC AGCAATGGAT CAATGAGAAG GAAGCCGCTC TGACAAGTGA GGAGGTCGGA 
GCAGACTTGG AGCAGGTTGA GGTGCTCCAG AAGAAGTTTG ATGACTTCCA GAAGGACCTG 
AAGGCCAATG AGTCACGGTT GAAGGACATT AACAAGGTAG CTGAAGACCT GGAGTCTGAA 
GGTCTCATGG CAGAGGAGGT GCAGGCTGTG CAACAACAGG AAGTGTATGG CATGATGCCC 
AGGGATGAAA CTGATTCCAA GACAGCCTCC CCGTGGAAGT CTGCTCGTCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTACAGCAG 
CTGGCCGAGG AACGGAGCCA GCTCTTGGGC AGCGCCCATG AAGTACAGAG GTTCCACAGA 
GATGCTGATG AAACCAAAGA ATGGATTGAA GAGAAGAATC AAGCTCTAAA CACAGACAAT 
TATGGACATG ATCTCGCCAG TGTCCAGGCC CTGCAACGCA AGCATGAGGG CTTCGAGAGG 
GACCTTGCGG CTCTCGGTGA CAAGGTAAAC TCCCTTGGTG AAACAGCAGA GCGCCTGATC 
CAGTCCCATC CCGAGTCAGC AGAAGACCTG CAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGAGCAGCC TGGGGAAACG TGCAGATCAG CGCAAGGCAA AGTTGGGTGA CTCCCACGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGGGGG 
TTGGTGTCCT CAGATGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCATT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATCGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGACAGC AGCTGTTGGC TCACGGACAC TATGCCAGCC CTGAGATCAA GCAGAAACTT 
GATATTCTTG ACCAGGAGCG TGCAGACCTG GAGAAGGCCT GGGTTCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAACT GCAGCTGTTC CATCGGGACT GTGAGCAAGC TGAGAACTGG 
ATGGCTGCCC GGGAGGCCTT CTTGAATACC GAAGACAAAG GAGACTCACT GGACAGCGTA 
GAGGCTCTGA TCAAAAAACA TGAAGACTTT GACAAAGCGA TTAACGTCCA GGAAGAGAAG 
ATTGCTGCTC TGCAGGCCTT TGCCGACCAG CTCATCGCTG CCGGCCATTA TGCCAAGGGA 
GACATTTCTA GCCGGCGCAA TGAGGTCTTG GACAGGTGGC GACGTCTGAA AGCCCAGATG 
ATTGAGAAAA GGTCAAAGCT AGGAGAATCT CAAACCCTCC AACAGTTCAG CCGGGATGTG 
GATGAGATTG AGGCTTGGAT CAGTGAAAAA TTGCAAACAG CGAGTGATGA GTCGTACAAG 
GATCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ACCAGAAGCA CCAGGCTTTT 
GAAGCAGAGC TGCATGCCAA CGCTGACCGG ATCCGTGGGG TTATCGACAT GGGCAACTCC 
CTCATTGAAC GTGGAGCCTG TGCCGGCAGT GAGGATGCTG TCAAGGCCCG CCTGGCTGCC 
TTAGCTGACC AGTGGCAGTT CTTGGTGCAA AAGTCAGCGG AAAAGAGCCA GAAACTGAAA 
GAAGCCAACA AGCAGCAGAA CTTCAACACA GGGATCAAGG ACTTTGACTT CTGGCTGTCT 
GAGGTGGAGG CCCTGCTGGC ATCCGAAGAT TATGGCAAAG ACCTAGCTTC TGTGAACAAC 
CTGCTGAAAA AGCATCAACT GCTGGAAGCA GATATATCTG CCCATGAGGA TCGCCTGAAG 
GACCTGAACA GCCAGGCAGA CAGCCTGATG ACCAGCAGTG CCTTCGACAC CTCCCAAGTA 
AAGGACAAGA GGGACACCAT CAACGGGCGC TTCCAGAAGA TCAAGAGCAT GGCGGCCTCC 
CGGCGAGCCA AGCTGAATGA ATCCCATCGC CTGCACCAGT TCTTCCGGGA CATGGATGAC 
GAGGAGTCCT GGATCAAGGA GAAGAAGCTG CTGGTGGGCT CAGAGGACTA CGGCCGGGAC 
CTAACCGGCG TGCAGAACCT GAGGAAGAAG CACAAGCGGC TGGAAGCAGA ACTGGCTGCG 
CATGAGCCGG CTATTCAGGG TGTCCTGGAC ACTGGCAAGA AGCTGTCCGA TGACAACACC 
ATCGGGAAAG AGGAGATCCA GCAGCGGCTG GCGCAGTTTG TGGAGCACTG GAAAGAGCTG 
AAGCAGCTGG CAGCTGCCCG GGGTCAGCGG CTGGAAGAGT CCTTGGAATA TCAGCAGTTT 
GTAGCCAATG TGGAAGAGGA AGAAGCCTGG ATCAATGAGA AAATGACCCT GGTGGCCAGC 
GAAGATTATG GCGACACTCT TGCCGCCATC CAGGGCTTAC TGAAGAAACA TGAAGCTTTT 
GAGACAGACT TCACCGTCCA CAAGGATCGC GTGAATGATG TCTGCACCAA TGGACAAGAC 
CTCATTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CAAAGATGAA GGGCCTGAAC 
GGGAAAGTGT CAGACCTGGA GAAAGCTGCA GCCCAGAGAA AGGCGAAGCT GGATGAGAAC 
TCGGCCTTCC TTCAGTTCAA CTGGAAGGCG GACGTGGTGG AGTCCTGGAT CGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGC CGAGACCTGT CTTCTGTGCA GACGCTCCTC 
ACCAAACAGG AAACTTTTGA CGCTGGGCTG CAGGCCTTCC AGCAGGAAGG CATTGCCAAC 
ATCACTGCCC TCAAAGATCA GCTTCTCGCC GCCAAACACG TTCAGTCCAA GGCCATCGAG 
GCCCGGCACG CCTCCCTCAT GAAGAGGTGG AGCCAGCTTC TGGCCAACTC AGCCGCCCGC 
AAGAAGAAGC TTCTGGAGGC TCAGAGTCAC TTCCGCAAGG TGGAGGACCT CTTCCTGACC 
TTCGCCAAAA AGGCTTCTGC CTTCAACAGC TGGTTTGAAA ATGCAGAGGA GGACTTAACA 
GACCCCGTGC GCTGCAACTC CTTGGAAGAA ATCAAAGCTT TGCGCGAGGC CCACGACGCC 
TTCCGCTCCT CCCTCAGCTC TGCCCAGGCT GACTTCAACC AGCTGGCCGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT AGCCTCCAAC CCCTACACCT GGTTTACCAT GGAGGCCCTG 
GAGGAGACCT GGAGGAACCT ACAGAAAATC ATCAAGGGTG TTGCTATGTT GCCCAGGCTG 
GTCTTCAACT CCTGGCCTCA AGCAGTCCTC CTGCCCAGGC CTCCCAAAGT GCTGGGATTA 
CAGGAGAGGG AGCTGGAGCT GCAGAAGGAA CAGCGGCGGC AGGAGGAGAA CGACAAGCTG 
CGCCAGGAGT TTGCCCAGCA CGCCAACGCC TTCCACCAGT GGATCCAAGA GACCAGGACA 
TACCTCCTCG ATGGGTCCTG TATGGTGGAA GAGTCGGGGA CCCTCGAATC CCAGCTTGAA 
GCTACCAAAC GCAAGCACCA GGAAATCCGA GCCATGAGAA GTCAGCTCAA AAAGATCGAG 
GACCTGGGGG CCGCCATGGA GGAGGCCCTC ATCCTGGACA ACAAGTACAC GGAGCACAGC 
ACCGTGGGCC TCGCCCAGCA GTGGGACCAG CTGGACCAGC TGGGCATGCG CATGCAGCAC 
AACCTGGAGC AGCAGATCCA GGCCAGGAAC ACAACAGGTG TGACTGAGGA GGCCCTCAAA 
GAATTCAGCA TGATGTTTAA ACACTTTGAC AAGGACAAGT CTGGCAGGCT GAACCATCAG 
GAGTTCAAAT CTTGCCTGCG CTCCCTGGGC TATGACCTGC CCATGGTGGA GGAAGGGGAA 
CCTGACCCTG AGTTCGAGGC AATCCTGGAC ACGGTGGATC CGAACAGAGA TGGCCATGTC 
TCCTTGCAAG AATACATGGC TTTCATGATC AGCCGCGAAA CTGAGAACGT CAAGTCCAGC 
GAGGAGATTG AGAGCGCCTT CCGGGCCCTC AGCTCAGAGG GAAAGCCTTA CGTGACCAAG 
GAGGAGCTCT ACCAGAACCT GACCCGGGAA CAAGCCGACT ACTGCGTCTC CCACATGAAG 
CCCTACGTGG ACGGCAAGGG CCGCGAGCTC CCCACCGCGT TCGACTACGT GGAGTTCACC 
CGCTCGCTTT TCGTGAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( NM_001375318.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001362247.1 Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant 9, mRNA.

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CloneID	OHu43453
Clone ID Related Accession (Same CDS sequence)	NM_001375318.1 , XM_006717245.1
Accession Version	NM_001375318.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7533bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	spectrin alpha chain, non-erythrocytic 1 isoform 9
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL356481.16. On Oct 23, 2019 this sequence version replaced XM_006717245.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.

ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACCG ATACCACCGC 
TTCAAGGAAC TCTCAACCCT TAGGCGTCAG AAGCTGGAAG ATTCCTATCG ATTCCAGTTC 
TTTCAAAGAG ATGCTGAAGA GCTGGAGAAA TGGATACAGG AAAAACTTCA GATTGCATCT 
GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGAAAGC TTCAGAAGCA TCAAGCATTT 
GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAGC TGGATGAAAC TGGAAACCTG 
ATGATCTCAG AAGGGCATTT TGCATCTGAA ACCATACGGA CCCGTTTGAT GGAGCTGCAC 
CGCCAGTGGG AATTACTTTT GGAGAAGATG CGAGAAAAAG GAATCAAACT GCTGCAGGCC 
CAGAAGTTGG TGCAGTACTT ACGAGAATGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCAATTG TTACTTCTGA AGAGCTGGGC CAGGATCTGG AGCATGTAGA GGTTTTACAG 
AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCTCATG AAGAAAGAGT TAATGAAGTG 
AACCAGTTTG CTGCCAAACT CATACAGGAG CAGCACCCTG AGGAGGAACT GATCAAGACT 
AAGCAGGATG AAGTCAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCTCT GCAGAGGCAG 
GGGAAGCTCT TTGGGGCAGC AGAAGTTCAG CGCTTTAACA GGGATGTGGA TGAGACTATC 
AGTTGGATTA AGGAAAAGGA GCAGTTAATG GCCTCTGATG ATTTTGGCCG AGACCTGGCA 
AGTGTTCAGG CTCTGCTTCG GAAGCACGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAA 
GACAAGGTCA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCCCA CCCTCTGAGT 
GCAACACAGA TTCAAGTGAA GCGAGAGGAA CTGATTACAA ACTGGGAGCA GATCCGCACC 
TTGGCGGCAG AGAGACATGC ACGGCTCAAT GATTCATACA GGCTTCAACG CTTCCTTGCT 
GACTTCCGTG ACCTCACCAG CTGGGTGACT GAGATGAAAG CCCTCATCAA TGCAGATGAG 
CTTGCCAGTG ATGTGGCTGG GGCTGAAGCC CTGCTAGATA GACACCAAGA GCACAAGGGT 
GAAATTGATG CCCATGAAGA CAGCTTCAAA TCTGCAGATG AATCTGGACA GGCACTGCTT 
GCTGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCGTCCT TTCCGAGGAG 
AGAGCGGCGC TGCTGGAGCT GTGGGAGCTG CGCAGGCAGC AGTACGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGGGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ATGAAGACTT GGGAGATTCC TTGGATAGTG TGGAAGCGCT TCTTAAGAAG 
CACGAAGACT TTGAGAAATC CCTTAGTGCC CAGGAGGAAA AGATTACAGC ATTAGATGAA 
TTTGCAACCA AGCTAATTCA GAACAACCAC TATGCAATGG AAGATGTGGC CACTCGCCGA 
GATGCTCTGT TGAGCCGCCG CAATGCCCTT CACGAGAGAG CCATGCGTCG CCGGGCCCAG 
CTAGCCGATT CTTTCCATCT GCAGCAGTTT TTCCGTGATT CTGATGAGCT CAAGAGTTGG 
GTCAATGAGA AGATGAAAAC TGCCACAGAT GAAGCTTATA AAGATCCATC CAACCTACAA 
GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CAGCAAACCA GAGCCGAATT 
GATGCCTTGG AGAAAGCTGG CCAAAAGCTG ATTGATGTCA ACCACTATGC CAAGGATGAA 
GTGGCAGCTC GTATGAATGA GGTGATCAGT TTGTGGAAGA AACTGCTAGA GGCCACTGAA 
CTGAAAGGAA TAAAGCTTCG TGAAGCCAAC CAGCAACAGC AATTTAATCG CAATGTTGAG 
GATATTGAAT TGTGGCTATA TGAAGTAGAA GGTCACTTGG CTTCGGATGA TTACGGCAAA 
GATCTTACCA ATGTGCAGAA CCTCCAGAAG AAACATGCAC TGCTAGAGGC AGATGTGGCT 
GCTCACCAGG ACCGAATTGA TGGCATCACC ATTCAGGCCC GCCAGTTCCA AGATGCTGGC 
CATTTTGATG CAGAAAACAT CAAGAAGAAA CAGGAAGCCC TCGTGGCTCG CTATGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCTCTGCG GTTGCAGCAG 
CTCTTCCGGG ATGTTGAGGA TGAGGAGACG TGGATTCGAG AGAAAGAGCC CATTGCCGCA 
TCTACCAACA GAGGTAAGGA TTTAATTGGG GTCCAGAATC TGCTAAAGAA ACATCAAGCC 
TTACAAGCAG AAATTGCTGG ACATGAACCA CGCATCAAAG CAGTTACACA GAAGGGGAAT 
GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAGCT TCACGAGCTG 
AACCAAAAGT GGGAGGCACT GAAAGCCAAA GCTTCCCAGC GTCGGCAGGA CCTGGAGGAC 
TCTCTGCAGG CCCAGCAGTA CTTTGCTGAT GCTAACGAGG CTGAATCCTG GATGCGGGAG 
AAGGAACCCA TTGTGGGCAG CACTGACTAT GGCAAGGACG AAGACTCTGC TGAGGCTCTA 
CTGAAGAAAC ACGAAGCTTT GATGTCAGAT CTCAGTGCCT ACGGCAGCAG CATCCAGGCT 
TTGCGAGAAC AAGCACAGTC CTGCCGGCAA CAAGTGGCCC CCACGGATGA TGAGACTGGG 
AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAGGGAG ATATCCTTAC CTTACTCAAC AGCACCAACA AGGATTGGTG GAAAGTGGAA 
GTGAACGATC GTCAGGGTTT TGTGCCGGCT GCGTACGTGA AGAAATTGGA CCCCGCCCAG 
TCAGCCTCCC GGGAGAATCT CCTGGAGGAG CAAGGCAGCA TAGCACTGCG GCAGGAGCAG 
ATTGACAATC AGACACGCAT AACTAAGGAG GCCGGCAGTG TATCTCTGCG TATGAAGCAG 
GTGGAAGAAC TATATCATTC TCTGCTGGAA CTGGGTGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGTTGCA AGAAGTTTAT GTTGTTCCGT GAAGCGAATG AACTACAGCA ATGGATCAAT 
GAGAAGGAAG CCGCTCTGAC AAGTGAGGAG GTCGGAGCAG ACTTGGAGCA GGTTGAGGTG 
CTCCAGAAGA AGTTTGATGA CTTCCAGAAG GACCTGAAGG CCAATGAGTC ACGGTTGAAG 
GACATTAACA AGGTAGCTGA AGACCTGGAG TCTGAAGGTC TCATGGCAGA GGAGGTGCAG 
GCTGTGCAAC AACAGGAAGT GTATGGCATG ATGCCCAGGG ATGAAACTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGTCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTGA ATGAGCGCTG GCGGTCCCTA CAGCAGCTGG CCGAGGAACG GAGCCAGCTC 
TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CTGATGAAAC CAAAGAATGG 
ATTGAAGAGA AGAATCAAGC TCTAAACACA GACAATTATG GACATGATCT CGCCAGTGTC 
CAGGCCCTGC AACGCAAGCA TGAGGGCTTC GAGAGGGACC TTGCGGCTCT CGGTGACAAG 
GTAAACTCCC TTGGTGAAAC AGCAGAGCGC CTGATCCAGT CCCATCCCGA GTCAGCAGAA 
GACCTGCAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA GCAGCCTGGG GAAACGTGCA 
GATCAGCGCA AGGCAAAGTT GGGTGACTCC CACGACCTGC AGCGCTTCCT TAGCGATTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATA CGGGGGTTGG TGTCCTCAGA TGAGCTAGCC 
AAGGATGTCA CCGGAGCTGA GGCATTGCTG GAGCGACACC AGGAACACCG GACAGAAATC 
GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GACAGCAGCT GTTGGCTCAC 
GGACACTATG CCAGCCCTGA GATCAAGCAG AAACTTGATA TTCTTGACCA GGAGCGTGCA 
GACCTGGAGA AGGCCTGGGT TCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAACTGCAG 
CTGTTCCATC GGGACTGTGA GCAAGCTGAG AACTGGATGG CTGCCCGGGA GGCCTTCTTG 
AATACCGAAG ACAAAGGAGA CTCACTGGAC AGCGTAGAGG CTCTGATCAA AAAACATGAA 
GACTTTGACA AAGCGATTAA CGTCCAGGAA GAGAAGATTG CTGCTCTGCA GGCCTTTGCC 
GACCAGCTCA TCGCTGCCGG CCATTATGCC AAGGGAGACA TTTCTAGCCG GCGCAATGAG 
GTCTTGGACA GGTGGCGACG TCTGAAAGCC CAGATGATTG AGAAAAGGTC AAAGCTAGGA 
GAATCTCAAA CCCTCCAACA GTTCAGCCGG GATGTGGATG AGATTGAGGC TTGGATCAGT 
GAAAAATTGC AAACAGCGAG TGATGAGTCG TACAAGGATC CCACCAACAT CCAGCTTTCC 
AAGCTGCTGA GCAAGCACCA GAAGCACCAG GCTTTTGAAG CAGAGCTGCA TGCCAACGCT 
GACCGGATCC GTGGGGTTAT CGACATGGGC AACTCCCTCA TTGAACGTGG AGCCTGTGCC 
GGCAGTGAGG ATGCTGTCAA GGCCCGCCTG GCTGCCTTAG CTGACCAGTG GCAGTTCTTG 
GTGCAAAAGT CAGCGGAAAA GAGCCAGAAA CTGAAAGAAG CCAACAAGCA GCAGAACTTC 
AACACAGGGA TCAAGGACTT TGACTTCTGG CTGTCTGAGG TGGAGGCCCT GCTGGCATCC 
GAAGATTATG GCAAAGACCT AGCTTCTGTG AACAACCTGC TGAAAAAGCA TCAACTGCTG 
GAAGCAGATA TATCTGCCCA TGAGGATCGC CTGAAGGACC TGAACAGCCA GGCAGACAGC 
CTGATGACCA GCAGTGCCTT CGACACCTCC CAAGTAAAGG ACAAGAGGGA CACCATCAAC 
GGGCGCTTCC AGAAGATCAA GAGCATGGCG GCCTCCCGGC GAGCCAAGCT GAATGAATCC 
CATCGCCTGC ACCAGTTCTT CCGGGACATG GATGACGAGG AGTCCTGGAT CAAGGAGAAG 
AAGCTGCTGG TGGGCTCAGA GGACTACGGC CGGGACCTAA CCGGCGTGCA GAACCTGAGG 
AAGAAGCACA AGCGGCTGGA AGCAGAACTG GCTGCGCATG AGCCGGCTAT TCAGGGTGTC 
CTGGACACTG GCAAGAAGCT GTCCGATGAC AACACCATCG GGAAAGAGGA GATCCAGCAG 
CGGCTGGCGC AGTTTGTGGA GCACTGGAAA GAGCTGAAGC AGCTGGCAGC TGCCCGGGGT 
CAGCGGCTGG AAGAGTCCTT GGAATATCAG CAGTTTGTAG CCAATGTGGA AGAGGAAGAA 
GCCTGGATCA ATGAGAAAAT GACCCTGGTG GCCAGCGAAG ATTATGGCGA CACTCTTGCC 
GCCATCCAGG GCTTACTGAA GAAACATGAA GCTTTTGAGA CAGACTTCAC CGTCCACAAG 
GATCGCGTGA ATGATGTCTG CACCAATGGA CAAGACCTCA TTAAGAAGAA CAATCACCAT 
GAGGAGAACA TCTCTTCAAA GATGAAGGGC CTGAACGGGA AAGTGTCAGA CCTGGAGAAA 
GCTGCAGCCC AGAGAAAGGC GAAGCTGGAT GAGAACTCGG CCTTCCTTCA GTTCAACTGG 
AAGGCGGACG TGGTGGAGTC CTGGATCGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT 
TATGGCCGAG ACCTGTCTTC TGTGCAGACG CTCCTCACCA AACAGGAAAC TTTTGACGCT 
GGGCTGCAGG CCTTCCAGCA GGAAGGCATT GCCAACATCA CTGCCCTCAA AGATCAGCTT 
CTCGCCGCCA AACACGTTCA GTCCAAGGCC ATCGAGGCCC GGCACGCCTC CCTCATGAAG 
AGGTGGAGCC AGCTTCTGGC CAACTCAGCC GCCCGCAAGA AGAAGCTTCT GGAGGCTCAG 
AGTCACTTCC GCAAGGTGGA GGACCTCTTC CTGACCTTCG CCAAAAAGGC TTCTGCCTTC 
AACAGCTGGT TTGAAAATGC AGAGGAGGAC TTAACAGACC CCGTGCGCTG CAACTCCTTG 
GAAGAAATCA AAGCTTTGCG CGAGGCCCAC GACGCCTTCC GCTCCTCCCT CAGCTCTGCC 
CAGGCTGACT TCAACCAGCT GGCCGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTAGCC 
TCCAACCCCT ACACCTGGTT TACCATGGAG GCCCTGGAGG AGACCTGGAG GAACCTACAG 
AAAATCATCA AGGAGAGGGA GCTGGAGCTG CAGAAGGAAC AGCGGCGGCA GGAGGAGAAC 
GACAAGCTGC GCCAGGAGTT TGCCCAGCAC GCCAACGCCT TCCACCAGTG GATCCAAGAG 
ACCAGGACAT ACCTCCTCGA TGGCATAGCA TATCGTCGGG TCATTCGTGT CTATCAGTAT 
GAAGTTGGGG ATGATCTGTC TGGAAGGTCC TGTATGGTGG AAGAGTCGGG GACCCTCGAA 
TCCCAGCTTG AAGCTACCAA ACGCAAGCAC CAGGAAATCC GAGCCATGAG AAGTCAGCTC 
AAAAAGATCG AGGACCTGGG GGCCGCCATG GAGGAGGCCC TCATCCTGGA CAACAAGTAC 
ACGGAGCACA GCACCGTGGG CCTCGCCCAG CAGTGGGACC AGCTGGACCA GCTGGGCATG 
CGCATGCAGC ACAACCTGGA GCAGCAGATC CAGGCCAGGA ACACAACAGG TGTGACTGAG 
GAGGCCCTCA AAGAATTCAG CATGATGTTT AAACACTTTG ACAAGGACAA GTCTGGCAGG 
CTGAACCATC AGGAGTTCAA ATCTTGCCTG CGCTCCCTGG GCTATGACCT GCCCATGGTG 
GAGGAAGGGG AACCTGACCC TGAGTTCGAG GCAATCCTGG ACACGGTGGA TCCGAACAGA 
GATGGCCATG TCTCCTTGCA AGAATACATG GCTTTCATGA TCAGCCGCGA AACTGAGAAC 
GTCAAGTCCA GCGAGGAGAT TGAGAGCGCC TTCCGGGCCC TCAGCTCAGA GGGAAAGCCT 
TACGTGACCA AGGAGGAGCT CTACCAGAAC CTGACCCGGG AACAAGCCGA CTACTGCGTC 
TCCCACATGA AGCCCTACGT GGACGGCAAG GGCCGCGAGC TCCCCACCGC GTTCGACTAC 
GTGGAGTTCA CCCGCTCGCT TTTCGTGAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001362247.1
CDS	744..8276
Translation	MLSSFRKRRVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELASDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMRRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPTDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRITKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGMMPRDETDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKQKLDILDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAAGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINGRFQKIKSMAASRRAKLNESHRLHQFFRDMDDEESWIKEKKLLVGSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHASLMKRWSQLLANSAARKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGIAYRRVIRVYQYEVGDDLSGRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	NM_001375318.1
Organism	Homo sapiens(human)
Definition	Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant 9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001375318.1

ORF Insert Sequence:

ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACCG ATACCACCGC 
TTCAAGGAAC TCTCAACCCT TAGGCGTCAG AAGCTGGAAG ATTCCTATCG ATTCCAGTTC 
TTTCAAAGAG ATGCTGAAGA GCTGGAGAAA TGGATACAGG AAAAACTTCA GATTGCATCT 
GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGAAAGC TTCAGAAGCA TCAAGCATTT 
GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAGC TGGATGAAAC TGGAAACCTG 
ATGATCTCAG AAGGGCATTT TGCATCTGAA ACCATACGGA CCCGTTTGAT GGAGCTGCAC 
CGCCAGTGGG AATTACTTTT GGAGAAGATG CGAGAAAAAG GAATCAAACT GCTGCAGGCC 
CAGAAGTTGG TGCAGTACTT ACGAGAATGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCAATTG TTACTTCTGA AGAGCTGGGC CAGGATCTGG AGCATGTAGA GGTTTTACAG 
AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCTCATG AAGAAAGAGT TAATGAAGTG 
AACCAGTTTG CTGCCAAACT CATACAGGAG CAGCACCCTG AGGAGGAACT GATCAAGACT 
AAGCAGGATG AAGTCAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCTCT GCAGAGGCAG 
GGGAAGCTCT TTGGGGCAGC AGAAGTTCAG CGCTTTAACA GGGATGTGGA TGAGACTATC 
AGTTGGATTA AGGAAAAGGA GCAGTTAATG GCCTCTGATG ATTTTGGCCG AGACCTGGCA 
AGTGTTCAGG CTCTGCTTCG GAAGCACGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAA 
GACAAGGTCA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCCCA CCCTCTGAGT 
GCAACACAGA TTCAAGTGAA GCGAGAGGAA CTGATTACAA ACTGGGAGCA GATCCGCACC 
TTGGCGGCAG AGAGACATGC ACGGCTCAAT GATTCATACA GGCTTCAACG CTTCCTTGCT 
GACTTCCGTG ACCTCACCAG CTGGGTGACT GAGATGAAAG CCCTCATCAA TGCAGATGAG 
CTTGCCAGTG ATGTGGCTGG GGCTGAAGCC CTGCTAGATA GACACCAAGA GCACAAGGGT 
GAAATTGATG CCCATGAAGA CAGCTTCAAA TCTGCAGATG AATCTGGACA GGCACTGCTT 
GCTGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCGTCCT TTCCGAGGAG 
AGAGCGGCGC TGCTGGAGCT GTGGGAGCTG CGCAGGCAGC AGTACGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGGGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ATGAAGACTT GGGAGATTCC TTGGATAGTG TGGAAGCGCT TCTTAAGAAG 
CACGAAGACT TTGAGAAATC CCTTAGTGCC CAGGAGGAAA AGATTACAGC ATTAGATGAA 
TTTGCAACCA AGCTAATTCA GAACAACCAC TATGCAATGG AAGATGTGGC CACTCGCCGA 
GATGCTCTGT TGAGCCGCCG CAATGCCCTT CACGAGAGAG CCATGCGTCG CCGGGCCCAG 
CTAGCCGATT CTTTCCATCT GCAGCAGTTT TTCCGTGATT CTGATGAGCT CAAGAGTTGG 
GTCAATGAGA AGATGAAAAC TGCCACAGAT GAAGCTTATA AAGATCCATC CAACCTACAA 
GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CAGCAAACCA GAGCCGAATT 
GATGCCTTGG AGAAAGCTGG CCAAAAGCTG ATTGATGTCA ACCACTATGC CAAGGATGAA 
GTGGCAGCTC GTATGAATGA GGTGATCAGT TTGTGGAAGA AACTGCTAGA GGCCACTGAA 
CTGAAAGGAA TAAAGCTTCG TGAAGCCAAC CAGCAACAGC AATTTAATCG CAATGTTGAG 
GATATTGAAT TGTGGCTATA TGAAGTAGAA GGTCACTTGG CTTCGGATGA TTACGGCAAA 
GATCTTACCA ATGTGCAGAA CCTCCAGAAG AAACATGCAC TGCTAGAGGC AGATGTGGCT 
GCTCACCAGG ACCGAATTGA TGGCATCACC ATTCAGGCCC GCCAGTTCCA AGATGCTGGC 
CATTTTGATG CAGAAAACAT CAAGAAGAAA CAGGAAGCCC TCGTGGCTCG CTATGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCTCTGCG GTTGCAGCAG 
CTCTTCCGGG ATGTTGAGGA TGAGGAGACG TGGATTCGAG AGAAAGAGCC CATTGCCGCA 
TCTACCAACA GAGGTAAGGA TTTAATTGGG GTCCAGAATC TGCTAAAGAA ACATCAAGCC 
TTACAAGCAG AAATTGCTGG ACATGAACCA CGCATCAAAG CAGTTACACA GAAGGGGAAT 
GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAGCT TCACGAGCTG 
AACCAAAAGT GGGAGGCACT GAAAGCCAAA GCTTCCCAGC GTCGGCAGGA CCTGGAGGAC 
TCTCTGCAGG CCCAGCAGTA CTTTGCTGAT GCTAACGAGG CTGAATCCTG GATGCGGGAG 
AAGGAACCCA TTGTGGGCAG CACTGACTAT GGCAAGGACG AAGACTCTGC TGAGGCTCTA 
CTGAAGAAAC ACGAAGCTTT GATGTCAGAT CTCAGTGCCT ACGGCAGCAG CATCCAGGCT 
TTGCGAGAAC AAGCACAGTC CTGCCGGCAA CAAGTGGCCC CCACGGATGA TGAGACTGGG 
AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAGGGAG ATATCCTTAC CTTACTCAAC AGCACCAACA AGGATTGGTG GAAAGTGGAA 
GTGAACGATC GTCAGGGTTT TGTGCCGGCT GCGTACGTGA AGAAATTGGA CCCCGCCCAG 
TCAGCCTCCC GGGAGAATCT CCTGGAGGAG CAAGGCAGCA TAGCACTGCG GCAGGAGCAG 
ATTGACAATC AGACACGCAT AACTAAGGAG GCCGGCAGTG TATCTCTGCG TATGAAGCAG 
GTGGAAGAAC TATATCATTC TCTGCTGGAA CTGGGTGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGTTGCA AGAAGTTTAT GTTGTTCCGT GAAGCGAATG AACTACAGCA ATGGATCAAT 
GAGAAGGAAG CCGCTCTGAC AAGTGAGGAG GTCGGAGCAG ACTTGGAGCA GGTTGAGGTG 
CTCCAGAAGA AGTTTGATGA CTTCCAGAAG GACCTGAAGG CCAATGAGTC ACGGTTGAAG 
GACATTAACA AGGTAGCTGA AGACCTGGAG TCTGAAGGTC TCATGGCAGA GGAGGTGCAG 
GCTGTGCAAC AACAGGAAGT GTATGGCATG ATGCCCAGGG ATGAAACTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGTCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTGA ATGAGCGCTG GCGGTCCCTA CAGCAGCTGG CCGAGGAACG GAGCCAGCTC 
TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CTGATGAAAC CAAAGAATGG 
ATTGAAGAGA AGAATCAAGC TCTAAACACA GACAATTATG GACATGATCT CGCCAGTGTC 
CAGGCCCTGC AACGCAAGCA TGAGGGCTTC GAGAGGGACC TTGCGGCTCT CGGTGACAAG 
GTAAACTCCC TTGGTGAAAC AGCAGAGCGC CTGATCCAGT CCCATCCCGA GTCAGCAGAA 
GACCTGCAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA GCAGCCTGGG GAAACGTGCA 
GATCAGCGCA AGGCAAAGTT GGGTGACTCC CACGACCTGC AGCGCTTCCT TAGCGATTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATA CGGGGGTTGG TGTCCTCAGA TGAGCTAGCC 
AAGGATGTCA CCGGAGCTGA GGCATTGCTG GAGCGACACC AGGAACACCG GACAGAAATC 
GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GACAGCAGCT GTTGGCTCAC 
GGACACTATG CCAGCCCTGA GATCAAGCAG AAACTTGATA TTCTTGACCA GGAGCGTGCA 
GACCTGGAGA AGGCCTGGGT TCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAACTGCAG 
CTGTTCCATC GGGACTGTGA GCAAGCTGAG AACTGGATGG CTGCCCGGGA GGCCTTCTTG 
AATACCGAAG ACAAAGGAGA CTCACTGGAC AGCGTAGAGG CTCTGATCAA AAAACATGAA 
GACTTTGACA AAGCGATTAA CGTCCAGGAA GAGAAGATTG CTGCTCTGCA GGCCTTTGCC 
GACCAGCTCA TCGCTGCCGG CCATTATGCC AAGGGAGACA TTTCTAGCCG GCGCAATGAG 
GTCTTGGACA GGTGGCGACG TCTGAAAGCC CAGATGATTG AGAAAAGGTC AAAGCTAGGA 
GAATCTCAAA CCCTCCAACA GTTCAGCCGG GATGTGGATG AGATTGAGGC TTGGATCAGT 
GAAAAATTGC AAACAGCGAG TGATGAGTCG TACAAGGATC CCACCAACAT CCAGCTTTCC 
AAGCTGCTGA GCAAGCACCA GAAGCACCAG GCTTTTGAAG CAGAGCTGCA TGCCAACGCT 
GACCGGATCC GTGGGGTTAT CGACATGGGC AACTCCCTCA TTGAACGTGG AGCCTGTGCC 
GGCAGTGAGG ATGCTGTCAA GGCCCGCCTG GCTGCCTTAG CTGACCAGTG GCAGTTCTTG 
GTGCAAAAGT CAGCGGAAAA GAGCCAGAAA CTGAAAGAAG CCAACAAGCA GCAGAACTTC 
AACACAGGGA TCAAGGACTT TGACTTCTGG CTGTCTGAGG TGGAGGCCCT GCTGGCATCC 
GAAGATTATG GCAAAGACCT AGCTTCTGTG AACAACCTGC TGAAAAAGCA TCAACTGCTG 
GAAGCAGATA TATCTGCCCA TGAGGATCGC CTGAAGGACC TGAACAGCCA GGCAGACAGC 
CTGATGACCA GCAGTGCCTT CGACACCTCC CAAGTAAAGG ACAAGAGGGA CACCATCAAC 
GGGCGCTTCC AGAAGATCAA GAGCATGGCG GCCTCCCGGC GAGCCAAGCT GAATGAATCC 
CATCGCCTGC ACCAGTTCTT CCGGGACATG GATGACGAGG AGTCCTGGAT CAAGGAGAAG 
AAGCTGCTGG TGGGCTCAGA GGACTACGGC CGGGACCTAA CCGGCGTGCA GAACCTGAGG 
AAGAAGCACA AGCGGCTGGA AGCAGAACTG GCTGCGCATG AGCCGGCTAT TCAGGGTGTC 
CTGGACACTG GCAAGAAGCT GTCCGATGAC AACACCATCG GGAAAGAGGA GATCCAGCAG 
CGGCTGGCGC AGTTTGTGGA GCACTGGAAA GAGCTGAAGC AGCTGGCAGC TGCCCGGGGT 
CAGCGGCTGG AAGAGTCCTT GGAATATCAG CAGTTTGTAG CCAATGTGGA AGAGGAAGAA 
GCCTGGATCA ATGAGAAAAT GACCCTGGTG GCCAGCGAAG ATTATGGCGA CACTCTTGCC 
GCCATCCAGG GCTTACTGAA GAAACATGAA GCTTTTGAGA CAGACTTCAC CGTCCACAAG 
GATCGCGTGA ATGATGTCTG CACCAATGGA CAAGACCTCA TTAAGAAGAA CAATCACCAT 
GAGGAGAACA TCTCTTCAAA GATGAAGGGC CTGAACGGGA AAGTGTCAGA CCTGGAGAAA 
GCTGCAGCCC AGAGAAAGGC GAAGCTGGAT GAGAACTCGG CCTTCCTTCA GTTCAACTGG 
AAGGCGGACG TGGTGGAGTC CTGGATCGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT 
TATGGCCGAG ACCTGTCTTC TGTGCAGACG CTCCTCACCA AACAGGAAAC TTTTGACGCT 
GGGCTGCAGG CCTTCCAGCA GGAAGGCATT GCCAACATCA CTGCCCTCAA AGATCAGCTT 
CTCGCCGCCA AACACGTTCA GTCCAAGGCC ATCGAGGCCC GGCACGCCTC CCTCATGAAG 
AGGTGGAGCC AGCTTCTGGC CAACTCAGCC GCCCGCAAGA AGAAGCTTCT GGAGGCTCAG 
AGTCACTTCC GCAAGGTGGA GGACCTCTTC CTGACCTTCG CCAAAAAGGC TTCTGCCTTC 
AACAGCTGGT TTGAAAATGC AGAGGAGGAC TTAACAGACC CCGTGCGCTG CAACTCCTTG 
GAAGAAATCA AAGCTTTGCG CGAGGCCCAC GACGCCTTCC GCTCCTCCCT CAGCTCTGCC 
CAGGCTGACT TCAACCAGCT GGCCGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTAGCC 
TCCAACCCCT ACACCTGGTT TACCATGGAG GCCCTGGAGG AGACCTGGAG GAACCTACAG 
AAAATCATCA AGGAGAGGGA GCTGGAGCTG CAGAAGGAAC AGCGGCGGCA GGAGGAGAAC 
GACAAGCTGC GCCAGGAGTT TGCCCAGCAC GCCAACGCCT TCCACCAGTG GATCCAAGAG 
ACCAGGACAT ACCTCCTCGA TGGCATAGCA TATCGTCGGG TCATTCGTGT CTATCAGTAT 
GAAGTTGGGG ATGATCTGTC TGGAAGGTCC TGTATGGTGG AAGAGTCGGG GACCCTCGAA 
TCCCAGCTTG AAGCTACCAA ACGCAAGCAC CAGGAAATCC GAGCCATGAG AAGTCAGCTC 
AAAAAGATCG AGGACCTGGG GGCCGCCATG GAGGAGGCCC TCATCCTGGA CAACAAGTAC 
ACGGAGCACA GCACCGTGGG CCTCGCCCAG CAGTGGGACC AGCTGGACCA GCTGGGCATG 
CGCATGCAGC ACAACCTGGA GCAGCAGATC CAGGCCAGGA ACACAACAGG TGTGACTGAG 
GAGGCCCTCA AAGAATTCAG CATGATGTTT AAACACTTTG ACAAGGACAA GTCTGGCAGG 
CTGAACCATC AGGAGTTCAA ATCTTGCCTG CGCTCCCTGG GCTATGACCT GCCCATGGTG 
GAGGAAGGGG AACCTGACCC TGAGTTCGAG GCAATCCTGG ACACGGTGGA TCCGAACAGA 
GATGGCCATG TCTCCTTGCA AGAATACATG GCTTTCATGA TCAGCCGCGA AACTGAGAAC 
GTCAAGTCCA GCGAGGAGAT TGAGAGCGCC TTCCGGGCCC TCAGCTCAGA GGGAAAGCCT 
TACGTGACCA AGGAGGAGCT CTACCAGAAC CTGACCCGGG AACAAGCCGA CTACTGCGTC 
TCCCACATGA AGCCCTACGT GGACGGCAAG GGCCGCGAGC TCCCCACCGC GTTCGACTAC 
GTGGAGTTCA CCCGCTCGCT TTTCGTGAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( NM_001375312.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001362241.2 Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant 8, mRNA.

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CloneID	OHu43456
Clone ID Related Accession (Same CDS sequence)	XM_006717248.2 , NM_001375312.2
Accession Version	NM_001375312.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7470bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	spectrin alpha chain, non-erythrocytic 1 isoform 8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL356481.16. On or before Oct 23, 2019 this sequence version replaced XM_006717248.2, NM_001375312.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.215716.1 [ECO:0000332] ##Evidence-Data-END##

ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACCG ATACCACCGC 
TTCAAGGAAC TCTCAACCCT TAGGCGTCAG AAGCTGGAAG ATTCCTATCG ATTCCAGTTC 
TTTCAAAGAG ATGCTGAAGA GCTGGAGAAA TGGATACAGG AAAAACTTCA GATTGCATCT 
GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGAAAGC TTCAGAAGCA TCAAGCATTT 
GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAGC TGGATGAAAC TGGAAACCTG 
ATGATCTCAG AAGGGCATTT TGCATCTGAA ACCATACGGA CCCGTTTGAT GGAGCTGCAC 
CGCCAGTGGG AATTACTTTT GGAGAAGATG CGAGAAAAAG GAATCAAACT GCTGCAGGCC 
CAGAAGTTGG TGCAGTACTT ACGAGAATGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCAATTG TTACTTCTGA AGAGCTGGGC CAGGATCTGG AGCATGTAGA GGTTTTACAG 
AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCTCATG AAGAAAGAGT TAATGAAGTG 
AACCAGTTTG CTGCCAAACT CATACAGGAG CAGCACCCTG AGGAGGAACT GATCAAGACT 
AAGCAGGATG AAGTCAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCTCT GCAGAGGCAG 
GGGAAGCTCT TTGGGGCAGC AGAAGTTCAG CGCTTTAACA GGGATGTGGA TGAGACTATC 
AGTTGGATTA AGGAAAAGGA GCAGTTAATG GCCTCTGATG ATTTTGGCCG AGACCTGGCA 
AGTGTTCAGG CTCTGCTTCG GAAGCACGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAA 
GACAAGGTCA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCCCA CCCTCTGAGT 
GCAACACAGA TTCAAGTGAA GCGAGAGGAA CTGATTACAA ACTGGGAGCA GATCCGCACC 
TTGGCGGCAG AGAGACATGC ACGGCTCAAT GATTCATACA GGCTTCAACG CTTCCTTGCT 
GACTTCCGTG ACCTCACCAG CTGGGTGACT GAGATGAAAG CCCTCATCAA TGCAGATGAG 
CTTGCCAGTG ATGTGGCTGG GGCTGAAGCC CTGCTAGATA GACACCAAGA GCACAAGGGT 
GAAATTGATG CCCATGAAGA CAGCTTCAAA TCTGCAGATG AATCTGGACA GGCACTGCTT 
GCTGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCGTCCT TTCCGAGGAG 
AGAGCGGCGC TGCTGGAGCT GTGGGAGCTG CGCAGGCAGC AGTACGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGGGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ATGAAGACTT GGGAGATTCC TTGGATAGTG TGGAAGCGCT TCTTAAGAAG 
CACGAAGACT TTGAGAAATC CCTTAGTGCC CAGGAGGAAA AGATTACAGC ATTAGATGAA 
TTTGCAACCA AGCTAATTCA GAACAACCAC TATGCAATGG AAGATGTGGC CACTCGCCGA 
GATGCTCTGT TGAGCCGCCG CAATGCCCTT CACGAGAGAG CCATGCGTCG CCGGGCCCAG 
CTAGCCGATT CTTTCCATCT GCAGCAGTTT TTCCGTGATT CTGATGAGCT CAAGAGTTGG 
GTCAATGAGA AGATGAAAAC TGCCACAGAT GAAGCTTATA AAGATCCATC CAACCTACAA 
GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CAGCAAACCA GAGCCGAATT 
GATGCCTTGG AGAAAGCTGG CCAAAAGCTG ATTGATGTCA ACCACTATGC CAAGGATGAA 
GTGGCAGCTC GTATGAATGA GGTGATCAGT TTGTGGAAGA AACTGCTAGA GGCCACTGAA 
CTGAAAGGAA TAAAGCTTCG TGAAGCCAAC CAGCAACAGC AATTTAATCG CAATGTTGAG 
GATATTGAAT TGTGGCTATA TGAAGTAGAA GGTCACTTGG CTTCGGATGA TTACGGCAAA 
GATCTTACCA ATGTGCAGAA CCTCCAGAAG AAACATGCAC TGCTAGAGGC AGATGTGGCT 
GCTCACCAGG ACCGAATTGA TGGCATCACC ATTCAGGCCC GCCAGTTCCA AGATGCTGGC 
CATTTTGATG CAGAAAACAT CAAGAAGAAA CAGGAAGCCC TCGTGGCTCG CTATGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCTCTGCG GTTGCAGCAG 
CTCTTCCGGG ATGTTGAGGA TGAGGAGACG TGGATTCGAG AGAAAGAGCC CATTGCCGCA 
TCTACCAACA GAGGTAAGGA TTTAATTGGG GTCCAGAATC TGCTAAAGAA ACATCAAGCC 
TTACAAGCAG AAATTGCTGG ACATGAACCA CGCATCAAAG CAGTTACACA GAAGGGGAAT 
GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAGCT TCACGAGCTG 
AACCAAAAGT GGGAGGCACT GAAAGCCAAA GCTTCCCAGC GTCGGCAGGA CCTGGAGGAC 
TCTCTGCAGG CCCAGCAGTA CTTTGCTGAT GCTAACGAGG CTGAATCCTG GATGCGGGAG 
AAGGAACCCA TTGTGGGCAG CACTGACTAT GGCAAGGACG AAGACTCTGC TGAGGCTCTA 
CTGAAGAAAC ACGAAGCTTT GATGTCAGAT CTCAGTGCCT ACGGCAGCAG CATCCAGGCT 
TTGCGAGAAC AAGCACAGTC CTGCCGGCAA CAAGTGGCCC CCACGGATGA TGAGACTGGG 
AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAGGGAG ATATCCTTAC CTTACTCAAC AGCACCAACA AGGATTGGTG GAAAGTGGAA 
GTGAACGATC GTCAGGGTTT TGTGCCGGCT GCGTACGTGA AGAAATTGGA CCCCGCCCAG 
TCAGCCTCCC GGGAGAATCT CCTGGAGGAG CAAGGCAGCA TAGCACTGCG GCAGGAGCAG 
ATTGACAATC AGACACGCAT AACTAAGGAG GCCGGCAGTG TATCTCTGCG TATGAAGCAG 
GTGGAAGAAC TATATCATTC TCTGCTGGAA CTGGGTGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGTTGCA AGAAGTTTAT GTTGTTCCGT GAAGCGAATG AACTACAGCA ATGGATCAAT 
GAGAAGGAAG CCGCTCTGAC AAGTGAGGAG GTCGGAGCAG ACTTGGAGCA GGTTGAGGTG 
CTCCAGAAGA AGTTTGATGA CTTCCAGAAG GACCTGAAGG CCAATGAGTC ACGGTTGAAG 
GACATTAACA AGGTAGCTGA AGACCTGGAG TCTGAAGGTC TCATGGCAGA GGAGGTGCAG 
GCTGTGCAAC AACAGGAAGT GTATGGCATG ATGCCCAGGG ATGAAACTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGTCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTGA ATGAGCGCTG GCGGTCCCTA CAGCAGCTGG CCGAGGAACG GAGCCAGCTC 
TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CTGATGAAAC CAAAGAATGG 
ATTGAAGAGA AGAATCAAGC TCTAAACACA GACAATTATG GACATGATCT CGCCAGTGTC 
CAGGCCCTGC AACGCAAGCA TGAGGGCTTC GAGAGGGACC TTGCGGCTCT CGGTGACAAG 
GTAAACTCCC TTGGTGAAAC AGCAGAGCGC CTGATCCAGT CCCATCCCGA GTCAGCAGAA 
GACCTGCAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA GCAGCCTGGG GAAACGTGCA 
GATCAGCGCA AGGCAAAGTT GGGTGACTCC CACGACCTGC AGCGCTTCCT TAGCGATTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATA CGGGGGTTGG TGTCCTCAGA TGAGCTAGCC 
AAGGATGTCA CCGGAGCTGA GGCATTGCTG GAGCGACACC AGGAACACCG GACAGAAATC 
GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GACAGCAGCT GTTGGCTCAC 
GGACACTATG CCAGCCCTGA GATCAAGCAG AAACTTGATA TTCTTGACCA GGAGCGTGCA 
GACCTGGAGA AGGCCTGGGT TCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAACTGCAG 
CTGTTCCATC GGGACTGTGA GCAAGCTGAG AACTGGATGG CTGCCCGGGA GGCCTTCTTG 
AATACCGAAG ACAAAGGAGA CTCACTGGAC AGCGTAGAGG CTCTGATCAA AAAACATGAA 
GACTTTGACA AAGCGATTAA CGTCCAGGAA GAGAAGATTG CTGCTCTGCA GGCCTTTGCC 
GACCAGCTCA TCGCTGCCGG CCATTATGCC AAGGGAGACA TTTCTAGCCG GCGCAATGAG 
GTCTTGGACA GGTGGCGACG TCTGAAAGCC CAGATGATTG AGAAAAGGTC AAAGCTAGGA 
GAATCTCAAA CCCTCCAACA GTTCAGCCGG GATGTGGATG AGATTGAGGC TTGGATCAGT 
GAAAAATTGC AAACAGCGAG TGATGAGTCG TACAAGGATC CCACCAACAT CCAGCTTTCC 
AAGCTGCTGA GCAAGCACCA GAAGCACCAG GCTTTTGAAG CAGAGCTGCA TGCCAACGCT 
GACCGGATCC GTGGGGTTAT CGACATGGGC AACTCCCTCA TTGAACGTGG AGCCTGTGCC 
GGCAGTGAGG ATGCTGTCAA GGCCCGCCTG GCTGCCTTAG CTGACCAGTG GCAGTTCTTG 
GTGCAAAAGT CAGCGGAAAA GAGCCAGAAA CTGAAAGAAG CCAACAAGCA GCAGAACTTC 
AACACAGGGA TCAAGGACTT TGACTTCTGG CTGTCTGAGG TGGAGGCCCT GCTGGCATCC 
GAAGATTATG GCAAAGACCT AGCTTCTGTG AACAACCTGC TGAAAAAGCA TCAACTGCTG 
GAAGCAGATA TATCTGCCCA TGAGGATCGC CTGAAGGACC TGAACAGCCA GGCAGACAGC 
CTGATGACCA GCAGTGCCTT CGACACCTCC CAAGTAAAGG ACAAGAGGGA CACCATCAAC 
GGGCGCTTCC AGAAGATCAA GAGCATGGCG GCCTCCCGGC GAGCCAAGCT GAATGAATCC 
CATCGCCTGC ACCAGTTCTT CCGGGACATG GATGACGAGG AGTCCTGGAT CAAGGAGAAG 
AAGCTGCTGG TGGGCTCAGA GGACTACGGC CGGGACCTAA CCGGCGTGCA GAACCTGAGG 
AAGAAGCACA AGCGGCTGGA AGCAGAACTG GCTGCGCATG AGCCGGCTAT TCAGGGTGTC 
CTGGACACTG GCAAGAAGCT GTCCGATGAC AACACCATCG GGAAAGAGGA GATCCAGCAG 
CGGCTGGCGC AGTTTGTGGA GCACTGGAAA GAGCTGAAGC AGCTGGCAGC TGCCCGGGGT 
CAGCGGCTGG AAGAGTCCTT GGAATATCAG CAGTTTGTAG CCAATGTGGA AGAGGAAGAA 
GCCTGGATCA ATGAGAAAAT GACCCTGGTG GCCAGCGAAG ATTATGGCGA CACTCTTGCC 
GCCATCCAGG GCTTACTGAA GAAACATGAA GCTTTTGAGA CAGACTTCAC CGTCCACAAG 
GATCGCGTGA ATGATGTCTG CACCAATGGA CAAGACCTCA TTAAGAAGAA CAATCACCAT 
GAGGAGAACA TCTCTTCAAA GATGAAGGGC CTGAACGGGA AAGTGTCAGA CCTGGAGAAA 
GCTGCAGCCC AGAGAAAGGC GAAGCTGGAT GAGAACTCGG CCTTCCTTCA GTTCAACTGG 
AAGGCGGACG TGGTGGAGTC CTGGATCGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT 
TATGGCCGAG ACCTGTCTTC TGTGCAGACG CTCCTCACCA AACAGGAAAC TTTTGACGCT 
GGGCTGCAGG CCTTCCAGCA GGAAGGCATT GCCAACATCA CTGCCCTCAA AGATCAGCTT 
CTCGCCGCCA AACACGTTCA GTCCAAGGCC ATCGAGGCCC GGCACGCCTC CCTCATGAAG 
AGGTGGAGCC AGCTTCTGGC CAACTCAGCC GCCCGCAAGA AGAAGCTTCT GGAGGCTCAG 
AGTCACTTCC GCAAGGTGGA GGACCTCTTC CTGACCTTCG CCAAAAAGGC TTCTGCCTTC 
AACAGCTGGT TTGAAAATGC AGAGGAGGAC TTAACAGACC CCGTGCGCTG CAACTCCTTG 
GAAGAAATCA AAGCTTTGCG CGAGGCCCAC GACGCCTTCC GCTCCTCCCT CAGCTCTGCC 
CAGGCTGACT TCAACCAGCT GGCCGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTAGCC 
TCCAACCCCT ACACCTGGTT TACCATGGAG GCCCTGGAGG AGACCTGGAG GAACCTACAG 
AAAATCATCA AGGAGAGGGA GCTGGAGCTG CAGAAGGAAC AGCGGCGGCA GGAGGAGAAC 
GACAAGCTGC GCCAGGAGTT TGCCCAGCAC GCCAACGCCT TCCACCAGTG GATCCAAGAG 
ACCAGGACAT ACCTCCTCGA TGGGTCCTGT ATGGTGGAAG AGTCGGGGAC CCTCGAATCC 
CAGCTTGAAG CTACCAAACG CAAGCACCAG GAAATCCGAG CCATGAGAAG TCAGCTCAAA 
AAGATCGAGG ACCTGGGGGC CGCCATGGAG GAGGCCCTCA TCCTGGACAA CAAGTACACG 
GAGCACAGCA CCGTGGGCCT CGCCCAGCAG TGGGACCAGC TGGACCAGCT GGGCATGCGC 
ATGCAGCACA ACCTGGAGCA GCAGATCCAG GCCAGGAACA CAACAGGTGT GACTGAGGAG 
GCCCTCAAAG AATTCAGCAT GATGTTTAAA CACTTTGACA AGGACAAGTC TGGCAGGCTG 
AACCATCAGG AGTTCAAATC TTGCCTGCGC TCCCTGGGCT ATGACCTGCC CATGGTGGAG 
GAAGGGGAAC CTGACCCTGA GTTCGAGGCA ATCCTGGACA CGGTGGATCC GAACAGAGAT 
GGCCATGTCT CCTTGCAAGA ATACATGGCT TTCATGATCA GCCGCGAAAC TGAGAACGTC 
AAGTCCAGCG AGGAGATTGA GAGCGCCTTC CGGGCCCTCA GCTCAGAGGG AAAGCCTTAC 
GTGACCAAGG AGGAGCTCTA CCAGAACCTG ACCCGGGAAC AAGCCGACTA CTGCGTCTCC 
CACATGAAGC CCTACGTGGA CGGCAAGGGC CGCGAGCTCC CCACCGCGTT CGACTACGTG 
GAGTTCACCC GCTCGCTTTT CGTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001362241.2
CDS	744..8213
Translation	MLSSFRKRRVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELASDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMRRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPTDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRITKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGMMPRDETDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKQKLDILDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAAGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINGRFQKIKSMAASRRAKLNESHRLHQFFRDMDDEESWIKEKKLLVGSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHASLMKRWSQLLANSAARKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	NM_001375312.2
Organism	Homo sapiens(human)
Definition	Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001375312.2

ORF Insert Sequence:

ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACCG ATACCACCGC 
TTCAAGGAAC TCTCAACCCT TAGGCGTCAG AAGCTGGAAG ATTCCTATCG ATTCCAGTTC 
TTTCAAAGAG ATGCTGAAGA GCTGGAGAAA TGGATACAGG AAAAACTTCA GATTGCATCT 
GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGAAAGC TTCAGAAGCA TCAAGCATTT 
GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAGC TGGATGAAAC TGGAAACCTG 
ATGATCTCAG AAGGGCATTT TGCATCTGAA ACCATACGGA CCCGTTTGAT GGAGCTGCAC 
CGCCAGTGGG AATTACTTTT GGAGAAGATG CGAGAAAAAG GAATCAAACT GCTGCAGGCC 
CAGAAGTTGG TGCAGTACTT ACGAGAATGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCAATTG TTACTTCTGA AGAGCTGGGC CAGGATCTGG AGCATGTAGA GGTTTTACAG 
AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCTCATG AAGAAAGAGT TAATGAAGTG 
AACCAGTTTG CTGCCAAACT CATACAGGAG CAGCACCCTG AGGAGGAACT GATCAAGACT 
AAGCAGGATG AAGTCAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCTCT GCAGAGGCAG 
GGGAAGCTCT TTGGGGCAGC AGAAGTTCAG CGCTTTAACA GGGATGTGGA TGAGACTATC 
AGTTGGATTA AGGAAAAGGA GCAGTTAATG GCCTCTGATG ATTTTGGCCG AGACCTGGCA 
AGTGTTCAGG CTCTGCTTCG GAAGCACGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAA 
GACAAGGTCA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCCCA CCCTCTGAGT 
GCAACACAGA TTCAAGTGAA GCGAGAGGAA CTGATTACAA ACTGGGAGCA GATCCGCACC 
TTGGCGGCAG AGAGACATGC ACGGCTCAAT GATTCATACA GGCTTCAACG CTTCCTTGCT 
GACTTCCGTG ACCTCACCAG CTGGGTGACT GAGATGAAAG CCCTCATCAA TGCAGATGAG 
CTTGCCAGTG ATGTGGCTGG GGCTGAAGCC CTGCTAGATA GACACCAAGA GCACAAGGGT 
GAAATTGATG CCCATGAAGA CAGCTTCAAA TCTGCAGATG AATCTGGACA GGCACTGCTT 
GCTGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCGTCCT TTCCGAGGAG 
AGAGCGGCGC TGCTGGAGCT GTGGGAGCTG CGCAGGCAGC AGTACGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGGGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ATGAAGACTT GGGAGATTCC TTGGATAGTG TGGAAGCGCT TCTTAAGAAG 
CACGAAGACT TTGAGAAATC CCTTAGTGCC CAGGAGGAAA AGATTACAGC ATTAGATGAA 
TTTGCAACCA AGCTAATTCA GAACAACCAC TATGCAATGG AAGATGTGGC CACTCGCCGA 
GATGCTCTGT TGAGCCGCCG CAATGCCCTT CACGAGAGAG CCATGCGTCG CCGGGCCCAG 
CTAGCCGATT CTTTCCATCT GCAGCAGTTT TTCCGTGATT CTGATGAGCT CAAGAGTTGG 
GTCAATGAGA AGATGAAAAC TGCCACAGAT GAAGCTTATA AAGATCCATC CAACCTACAA 
GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CAGCAAACCA GAGCCGAATT 
GATGCCTTGG AGAAAGCTGG CCAAAAGCTG ATTGATGTCA ACCACTATGC CAAGGATGAA 
GTGGCAGCTC GTATGAATGA GGTGATCAGT TTGTGGAAGA AACTGCTAGA GGCCACTGAA 
CTGAAAGGAA TAAAGCTTCG TGAAGCCAAC CAGCAACAGC AATTTAATCG CAATGTTGAG 
GATATTGAAT TGTGGCTATA TGAAGTAGAA GGTCACTTGG CTTCGGATGA TTACGGCAAA 
GATCTTACCA ATGTGCAGAA CCTCCAGAAG AAACATGCAC TGCTAGAGGC AGATGTGGCT 
GCTCACCAGG ACCGAATTGA TGGCATCACC ATTCAGGCCC GCCAGTTCCA AGATGCTGGC 
CATTTTGATG CAGAAAACAT CAAGAAGAAA CAGGAAGCCC TCGTGGCTCG CTATGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCTCTGCG GTTGCAGCAG 
CTCTTCCGGG ATGTTGAGGA TGAGGAGACG TGGATTCGAG AGAAAGAGCC CATTGCCGCA 
TCTACCAACA GAGGTAAGGA TTTAATTGGG GTCCAGAATC TGCTAAAGAA ACATCAAGCC 
TTACAAGCAG AAATTGCTGG ACATGAACCA CGCATCAAAG CAGTTACACA GAAGGGGAAT 
GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAGCT TCACGAGCTG 
AACCAAAAGT GGGAGGCACT GAAAGCCAAA GCTTCCCAGC GTCGGCAGGA CCTGGAGGAC 
TCTCTGCAGG CCCAGCAGTA CTTTGCTGAT GCTAACGAGG CTGAATCCTG GATGCGGGAG 
AAGGAACCCA TTGTGGGCAG CACTGACTAT GGCAAGGACG AAGACTCTGC TGAGGCTCTA 
CTGAAGAAAC ACGAAGCTTT GATGTCAGAT CTCAGTGCCT ACGGCAGCAG CATCCAGGCT 
TTGCGAGAAC AAGCACAGTC CTGCCGGCAA CAAGTGGCCC CCACGGATGA TGAGACTGGG 
AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAGGGAG ATATCCTTAC CTTACTCAAC AGCACCAACA AGGATTGGTG GAAAGTGGAA 
GTGAACGATC GTCAGGGTTT TGTGCCGGCT GCGTACGTGA AGAAATTGGA CCCCGCCCAG 
TCAGCCTCCC GGGAGAATCT CCTGGAGGAG CAAGGCAGCA TAGCACTGCG GCAGGAGCAG 
ATTGACAATC AGACACGCAT AACTAAGGAG GCCGGCAGTG TATCTCTGCG TATGAAGCAG 
GTGGAAGAAC TATATCATTC TCTGCTGGAA CTGGGTGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGTTGCA AGAAGTTTAT GTTGTTCCGT GAAGCGAATG AACTACAGCA ATGGATCAAT 
GAGAAGGAAG CCGCTCTGAC AAGTGAGGAG GTCGGAGCAG ACTTGGAGCA GGTTGAGGTG 
CTCCAGAAGA AGTTTGATGA CTTCCAGAAG GACCTGAAGG CCAATGAGTC ACGGTTGAAG 
GACATTAACA AGGTAGCTGA AGACCTGGAG TCTGAAGGTC TCATGGCAGA GGAGGTGCAG 
GCTGTGCAAC AACAGGAAGT GTATGGCATG ATGCCCAGGG ATGAAACTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGTCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTGA ATGAGCGCTG GCGGTCCCTA CAGCAGCTGG CCGAGGAACG GAGCCAGCTC 
TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CTGATGAAAC CAAAGAATGG 
ATTGAAGAGA AGAATCAAGC TCTAAACACA GACAATTATG GACATGATCT CGCCAGTGTC 
CAGGCCCTGC AACGCAAGCA TGAGGGCTTC GAGAGGGACC TTGCGGCTCT CGGTGACAAG 
GTAAACTCCC TTGGTGAAAC AGCAGAGCGC CTGATCCAGT CCCATCCCGA GTCAGCAGAA 
GACCTGCAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA GCAGCCTGGG GAAACGTGCA 
GATCAGCGCA AGGCAAAGTT GGGTGACTCC CACGACCTGC AGCGCTTCCT TAGCGATTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATA CGGGGGTTGG TGTCCTCAGA TGAGCTAGCC 
AAGGATGTCA CCGGAGCTGA GGCATTGCTG GAGCGACACC AGGAACACCG GACAGAAATC 
GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GACAGCAGCT GTTGGCTCAC 
GGACACTATG CCAGCCCTGA GATCAAGCAG AAACTTGATA TTCTTGACCA GGAGCGTGCA 
GACCTGGAGA AGGCCTGGGT TCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAACTGCAG 
CTGTTCCATC GGGACTGTGA GCAAGCTGAG AACTGGATGG CTGCCCGGGA GGCCTTCTTG 
AATACCGAAG ACAAAGGAGA CTCACTGGAC AGCGTAGAGG CTCTGATCAA AAAACATGAA 
GACTTTGACA AAGCGATTAA CGTCCAGGAA GAGAAGATTG CTGCTCTGCA GGCCTTTGCC 
GACCAGCTCA TCGCTGCCGG CCATTATGCC AAGGGAGACA TTTCTAGCCG GCGCAATGAG 
GTCTTGGACA GGTGGCGACG TCTGAAAGCC CAGATGATTG AGAAAAGGTC AAAGCTAGGA 
GAATCTCAAA CCCTCCAACA GTTCAGCCGG GATGTGGATG AGATTGAGGC TTGGATCAGT 
GAAAAATTGC AAACAGCGAG TGATGAGTCG TACAAGGATC CCACCAACAT CCAGCTTTCC 
AAGCTGCTGA GCAAGCACCA GAAGCACCAG GCTTTTGAAG CAGAGCTGCA TGCCAACGCT 
GACCGGATCC GTGGGGTTAT CGACATGGGC AACTCCCTCA TTGAACGTGG AGCCTGTGCC 
GGCAGTGAGG ATGCTGTCAA GGCCCGCCTG GCTGCCTTAG CTGACCAGTG GCAGTTCTTG 
GTGCAAAAGT CAGCGGAAAA GAGCCAGAAA CTGAAAGAAG CCAACAAGCA GCAGAACTTC 
AACACAGGGA TCAAGGACTT TGACTTCTGG CTGTCTGAGG TGGAGGCCCT GCTGGCATCC 
GAAGATTATG GCAAAGACCT AGCTTCTGTG AACAACCTGC TGAAAAAGCA TCAACTGCTG 
GAAGCAGATA TATCTGCCCA TGAGGATCGC CTGAAGGACC TGAACAGCCA GGCAGACAGC 
CTGATGACCA GCAGTGCCTT CGACACCTCC CAAGTAAAGG ACAAGAGGGA CACCATCAAC 
GGGCGCTTCC AGAAGATCAA GAGCATGGCG GCCTCCCGGC GAGCCAAGCT GAATGAATCC 
CATCGCCTGC ACCAGTTCTT CCGGGACATG GATGACGAGG AGTCCTGGAT CAAGGAGAAG 
AAGCTGCTGG TGGGCTCAGA GGACTACGGC CGGGACCTAA CCGGCGTGCA GAACCTGAGG 
AAGAAGCACA AGCGGCTGGA AGCAGAACTG GCTGCGCATG AGCCGGCTAT TCAGGGTGTC 
CTGGACACTG GCAAGAAGCT GTCCGATGAC AACACCATCG GGAAAGAGGA GATCCAGCAG 
CGGCTGGCGC AGTTTGTGGA GCACTGGAAA GAGCTGAAGC AGCTGGCAGC TGCCCGGGGT 
CAGCGGCTGG AAGAGTCCTT GGAATATCAG CAGTTTGTAG CCAATGTGGA AGAGGAAGAA 
GCCTGGATCA ATGAGAAAAT GACCCTGGTG GCCAGCGAAG ATTATGGCGA CACTCTTGCC 
GCCATCCAGG GCTTACTGAA GAAACATGAA GCTTTTGAGA CAGACTTCAC CGTCCACAAG 
GATCGCGTGA ATGATGTCTG CACCAATGGA CAAGACCTCA TTAAGAAGAA CAATCACCAT 
GAGGAGAACA TCTCTTCAAA GATGAAGGGC CTGAACGGGA AAGTGTCAGA CCTGGAGAAA 
GCTGCAGCCC AGAGAAAGGC GAAGCTGGAT GAGAACTCGG CCTTCCTTCA GTTCAACTGG 
AAGGCGGACG TGGTGGAGTC CTGGATCGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT 
TATGGCCGAG ACCTGTCTTC TGTGCAGACG CTCCTCACCA AACAGGAAAC TTTTGACGCT 
GGGCTGCAGG CCTTCCAGCA GGAAGGCATT GCCAACATCA CTGCCCTCAA AGATCAGCTT 
CTCGCCGCCA AACACGTTCA GTCCAAGGCC ATCGAGGCCC GGCACGCCTC CCTCATGAAG 
AGGTGGAGCC AGCTTCTGGC CAACTCAGCC GCCCGCAAGA AGAAGCTTCT GGAGGCTCAG 
AGTCACTTCC GCAAGGTGGA GGACCTCTTC CTGACCTTCG CCAAAAAGGC TTCTGCCTTC 
AACAGCTGGT TTGAAAATGC AGAGGAGGAC TTAACAGACC CCGTGCGCTG CAACTCCTTG 
GAAGAAATCA AAGCTTTGCG CGAGGCCCAC GACGCCTTCC GCTCCTCCCT CAGCTCTGCC 
CAGGCTGACT TCAACCAGCT GGCCGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTAGCC 
TCCAACCCCT ACACCTGGTT TACCATGGAG GCCCTGGAGG AGACCTGGAG GAACCTACAG 
AAAATCATCA AGGAGAGGGA GCTGGAGCTG CAGAAGGAAC AGCGGCGGCA GGAGGAGAAC 
GACAAGCTGC GCCAGGAGTT TGCCCAGCAC GCCAACGCCT TCCACCAGTG GATCCAAGAG 
ACCAGGACAT ACCTCCTCGA TGGGTCCTGT ATGGTGGAAG AGTCGGGGAC CCTCGAATCC 
CAGCTTGAAG CTACCAAACG CAAGCACCAG GAAATCCGAG CCATGAGAAG TCAGCTCAAA 
AAGATCGAGG ACCTGGGGGC CGCCATGGAG GAGGCCCTCA TCCTGGACAA CAAGTACACG 
GAGCACAGCA CCGTGGGCCT CGCCCAGCAG TGGGACCAGC TGGACCAGCT GGGCATGCGC 
ATGCAGCACA ACCTGGAGCA GCAGATCCAG GCCAGGAACA CAACAGGTGT GACTGAGGAG 
GCCCTCAAAG AATTCAGCAT GATGTTTAAA CACTTTGACA AGGACAAGTC TGGCAGGCTG 
AACCATCAGG AGTTCAAATC TTGCCTGCGC TCCCTGGGCT ATGACCTGCC CATGGTGGAG 
GAAGGGGAAC CTGACCCTGA GTTCGAGGCA ATCCTGGACA CGGTGGATCC GAACAGAGAT 
GGCCATGTCT CCTTGCAAGA ATACATGGCT TTCATGATCA GCCGCGAAAC TGAGAACGTC 
AAGTCCAGCG AGGAGATTGA GAGCGCCTTC CGGGCCCTCA GCTCAGAGGG AAAGCCTTAC 
GTGACCAAGG AGGAGCTCTA CCAGAACCTG ACCCGGGAAC AAGCCGACTA CTGCGTCTCC 
CACATGAAGC CCTACGTGGA CGGCAAGGGC CGCGAGCTCC CCACCGCGTT CGACTACGTG 
GAGTTCACCC GCTCGCTTTT CGTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( NM_001375313.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001362242.1 Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant 11, mRNA.

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CloneID	OHu98855
Clone ID Related Accession (Same CDS sequence)	NM_001375313.1 , XM_017015059.1
Accession Version	NM_001375313.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7416bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	spectrin alpha chain, non-erythrocytic 1 isoform 7
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL356481.16. On Oct 22, 2019 this sequence version replaced XM_017015059.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.54249.1 [ECO:0000332] ##Evidence-Data-END##

ATGGACCCAA GTGGGGTCAA AGTGCTGGAA ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTAG ACCGATACCA CCGCTTCAAG GAACTCTCAA CCCTTAGGCG TCAGAAGCTG 
GAAGATTCCT ATCGATTCCA GTTCTTTCAA AGAGATGCTG AAGAGCTGGA GAAATGGATA 
CAGGAAAAAC TTCAGATTGC ATCTGATGAG AATTATAAAG ACCCAACCAA CTTGCAGGGA 
AAGCTTCAGA AGCATCAAGC ATTTGAAGCT GAAGTGCAGG CCAACTCAGG AGCCATTGTT 
AAGCTGGATG AAACTGGAAA CCTGATGATC TCAGAAGGGC ATTTTGCATC TGAAACCATA 
CGGACCCGTT TGATGGAGCT GCACCGCCAG TGGGAATTAC TTTTGGAGAA GATGCGAGAA 
AAAGGAATCA AACTGCTGCA GGCCCAGAAG TTGGTGCAGT ACTTACGAGA ATGTGAGGAC 
GTGATGGACT GGATCAATGA CAAGGAAGCA ATTGTTACTT CTGAAGAGCT GGGCCAGGAT 
CTGGAGCATG TAGAGGTTTT ACAGAAGAAA TTTGAAGAGT TTCAAACAGA TATGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTGAACCAG TTTGCTGCCA AACTCATACA GGAGCAGCAC 
CCTGAGGAGG AACTGATCAA GACTAAGCAG GATGAAGTCA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CTCTGCAGAG GCAGGGGAAG CTCTTTGGGG CAGCAGAAGT TCAGCGCTTT 
AACAGGGATG TGGATGAGAC TATCAGTTGG ATTAAGGAAA AGGAGCAGTT AATGGCCTCT 
GATGATTTTG GCCGAGACCT GGCAAGTGTT CAGGCTCTGC TTCGGAAGCA CGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAAGACAAG GTCAAAGCCC TGTGTGCTGA GGCTGACCGC 
CTGCAACAGT CCCACCCTCT GAGTGCAACA CAGATTCAAG TGAAGCGAGA GGAACTGATT 
ACAAACTGGG AGCAGATCCG CACCTTGGCG GCAGAGAGAC ATGCACGGCT CAATGATTCA 
TACAGGCTTC AACGCTTCCT TGCTGACTTC CGTGACCTCA CCAGCTGGGT GACTGAGATG 
AAAGCCCTCA TCAATGCAGA TGAGCTTGCC AGTGATGTGG CTGGGGCTGA AGCCCTGCTA 
GATAGACACC AAGAGCACAA GGGTGAAATT GATGCCCATG AAGACAGCTT CAAATCTGCA 
GATGAATCTG GACAGGCACT GCTTGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGACCG TCCTTTCCGA GGAGAGAGCG GCGCTGCTGG AGCTGTGGGA GCTGCGCAGG 
CAGCAGTACG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GGGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAATGAAG ACTTGGGAGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTTAA GAAGCACGAA GACTTTGAGA AATCCCTTAG TGCCCAGGAG 
GAAAAGATTA CAGCATTAGA TGAATTTGCA ACCAAGCTAA TTCAGAACAA CCACTATGCA 
ATGGAAGATG TGGCCACTCG CCGAGATGCT CTGTTGAGCC GCCGCAATGC CCTTCACGAG 
AGAGCCATGC GTCGCCGGGC CCAGCTAGCC GATTCTTTCC ATCTGCAGCA GTTTTTCCGT 
GATTCTGATG AGCTCAAGAG TTGGGTCAAT GAGAAGATGA AAACTGCCAC AGATGAAGCT 
TATAAAGATC CATCCAACCT ACAAGGAAAA GTACAGAAGC ATCAGGCTTT TGAGGCTGAG 
CTCTCAGCAA ACCAGAGCCG AATTGATGCC TTGGAGAAAG CTGGCCAAAA GCTGATTGAT 
GTCAACCACT ATGCCAAGGA TGAAGTGGCA GCTCGTATGA ATGAGGTGAT CAGTTTGTGG 
AAGAAACTGC TAGAGGCCAC TGAACTGAAA GGAATAAAGC TTCGTGAAGC CAACCAGCAA 
CAGCAATTTA ATCGCAATGT TGAGGATATT GAATTGTGGC TATATGAAGT AGAAGGTCAC 
TTGGCTTCGG ATGATTACGG CAAAGATCTT ACCAATGTGC AGAACCTCCA GAAGAAACAT 
GCACTGCTAG AGGCAGATGT GGCTGCTCAC CAGGACCGAA TTGATGGCAT CACCATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTT GATGCAGAAA ACATCAAGAA GAAACAGGAA 
GCCCTCGTGG CTCGCTATGA GGCACTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCCGATTCTC TGCGGTTGCA GCAGCTCTTC CGGGATGTTG AGGATGAGGA GACGTGGATT 
CGAGAGAAAG AGCCCATTGC CGCATCTACC AACAGAGGTA AGGATTTAAT TGGGGTCCAG 
AATCTGCTAA AGAAACATCA AGCCTTACAA GCAGAAATTG CTGGACATGA ACCACGCATC 
AAAGCAGTTA CACAGAAGGG GAATGCCATG GTGGAGGAAG GCCATTTTGC TGCAGAGGAT 
GTGAAGGCCA AGCTTCACGA GCTGAACCAA AAGTGGGAGG CACTGAAAGC CAAAGCTTCC 
CAGCGTCGGC AGGACCTGGA GGACTCTCTG CAGGCCCAGC AGTACTTTGC TGATGCTAAC 
GAGGCTGAAT CCTGGATGCG GGAGAAGGAA CCCATTGTGG GCAGCACTGA CTATGGCAAG 
GACGAAGACT CTGCTGAGGC TCTACTGAAG AAACACGAAG CTTTGATGTC AGATCTCAGT 
GCCTACGGCA GCAGCATCCA GGCTTTGCGA GAACAAGCAC AGTCCTGCCG GCAACAAGTG 
GCCCCCACGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CTCTCTACGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAG GGAGATATCC TTACCTTACT CAACAGCACC 
AACAAGGATT GGTGGAAAGT GGAAGTGAAC GATCGTCAGG GTTTTGTGCC GGCTGCGTAC 
GTGAAGAAAT TGGACCCCGC CCAGTCAGCC TCCCGGGAGA ATCTCCTGGA GGAGCAAGGC 
AGCATAGCAC TGCGGCAGGA GCAGATTGAC AATCAGACAC GCATAACTAA GGAGGCCGGC 
AGTGTATCTC TGCGTATGAA GCAGGTGGAA GAACTATATC ATTCTCTGCT GGAACTGGGT 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TTATGTTGTT CCGTGAAGCG 
AATGAACTAC AGCAATGGAT CAATGAGAAG GAAGCCGCTC TGACAAGTGA GGAGGTCGGA 
GCAGACTTGG AGCAGGTTGA GGTGCTCCAG AAGAAGTTTG ATGACTTCCA GAAGGACCTG 
AAGGCCAATG AGTCACGGTT GAAGGACATT AACAAGGTAG CTGAAGACCT GGAGTCTGAA 
GGTCTCATGG CAGAGGAGGT GCAGGCTGTG CAACAACAGG AAGTGTATGG CATGATGCCC 
AGGGATGAAA CTGATTCCAA GACAGCCTCC CCGTGGAAGT CTGCTCGTCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTACAGCAG 
CTGGCCGAGG AACGGAGCCA GCTCTTGGGC AGCGCCCATG AAGTACAGAG GTTCCACAGA 
GATGCTGATG AAACCAAAGA ATGGATTGAA GAGAAGAATC AAGCTCTAAA CACAGACAAT 
TATGGACATG ATCTCGCCAG TGTCCAGGCC CTGCAACGCA AGCATGAGGG CTTCGAGAGG 
GACCTTGCGG CTCTCGGTGA CAAGGTAAAC TCCCTTGGTG AAACAGCAGA GCGCCTGATC 
CAGTCCCATC CCGAGTCAGC AGAAGACCTG CAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGAGCAGCC TGGGGAAACG TGCAGATCAG CGCAAGGCAA AGTTGGGTGA CTCCCACGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGGGGG 
TTGGTGTCCT CAGATGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCATT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATCGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGACAGC AGCTGTTGGC TCACGGACAC TATGCCAGCC CTGAGATCAA GCAGAAACTT 
GATATTCTTG ACCAGGAGCG TGCAGACCTG GAGAAGGCCT GGGTTCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAACT GCAGCTGTTC CATCGGGACT GTGAGCAAGC TGAGAACTGG 
ATGGCTGCCC GGGAGGCCTT CTTGAATACC GAAGACAAAG GAGACTCACT GGACAGCGTA 
GAGGCTCTGA TCAAAAAACA TGAAGACTTT GACAAAGCGA TTAACGTCCA GGAAGAGAAG 
ATTGCTGCTC TGCAGGCCTT TGCCGACCAG CTCATCGCTG CCGGCCATTA TGCCAAGGGA 
GACATTTCTA GCCGGCGCAA TGAGGTCTTG GACAGGTGGC GACGTCTGAA AGCCCAGATG 
ATTGAGAAAA GGTCAAAGCT AGGAGAATCT CAAACCCTCC AACAGTTCAG CCGGGATGTG 
GATGAGATTG AGGCTTGGAT CAGTGAAAAA TTGCAAACAG CGAGTGATGA GTCGTACAAG 
GATCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ACCAGAAGCA CCAGGCTTTT 
GAAGCAGAGC TGCATGCCAA CGCTGACCGG ATCCGTGGGG TTATCGACAT GGGCAACTCC 
CTCATTGAAC GTGGAGCCTG TGCCGGCAGT GAGGATGCTG TCAAGGCCCG CCTGGCTGCC 
TTAGCTGACC AGTGGCAGTT CTTGGTGCAA AAGTCAGCGG AAAAGAGCCA GAAACTGAAA 
GAAGCCAACA AGCAGCAGAA CTTCAACACA GGGATCAAGG ACTTTGACTT CTGGCTGTCT 
GAGGTGGAGG CCCTGCTGGC ATCCGAAGAT TATGGCAAAG ACCTAGCTTC TGTGAACAAC 
CTGCTGAAAA AGCATCAACT GCTGGAAGCA GATATATCTG CCCATGAGGA TCGCCTGAAG 
GACCTGAACA GCCAGGCAGA CAGCCTGATG ACCAGCAGTG CCTTCGACAC CTCCCAAGTA 
AAGGACAAGA GGGACACCAT CAACGGGCGC TTCCAGAAGA TCAAGAGCAT GGCGGCCTCC 
CGGCGAGCCA AGCTGAATGA ATCCCATCGC CTGCACCAGT TCTTCCGGGA CATGGATGAC 
GAGGAGTCCT GGATCAAGGA GAAGAAGCTG CTGGTGGGCT CAGAGGACTA CGGCCGGGAC 
CTAACCGGCG TGCAGAACCT GAGGAAGAAG CACAAGCGGC TGGAAGCAGA ACTGGCTGCG 
CATGAGCCGG CTATTCAGGG TGTCCTGGAC ACTGGCAAGA AGCTGTCCGA TGACAACACC 
ATCGGGAAAG AGGAGATCCA GCAGCGGCTG GCGCAGTTTG TGGAGCACTG GAAAGAGCTG 
AAGCAGCTGG CAGCTGCCCG GGGTCAGCGG CTGGAAGAGT CCTTGGAATA TCAGCAGTTT 
GTAGCCAATG TGGAAGAGGA AGAAGCCTGG ATCAATGAGA AAATGACCCT GGTGGCCAGC 
GAAGATTATG GCGACACTCT TGCCGCCATC CAGGGCTTAC TGAAGAAACA TGAAGCTTTT 
GAGACAGACT TCACCGTCCA CAAGGATCGC GTGAATGATG TCTGCACCAA TGGACAAGAC 
CTCATTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CAAAGATGAA GGGCCTGAAC 
GGGAAAGTGT CAGACCTGGA GAAAGCTGCA GCCCAGAGAA AGGCGAAGCT GGATGAGAAC 
TCGGCCTTCC TTCAGTTCAA CTGGAAGGCG GACGTGGTGG AGTCCTGGAT CGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGC CGAGACCTGT CTTCTGTGCA GACGCTCCTC 
ACCAAACAGG AAACTTTTGA CGCTGGGCTG CAGGCCTTCC AGCAGGAAGG CATTGCCAAC 
ATCACTGCCC TCAAAGATCA GCTTCTCGCC GCCAAACACG TTCAGTCCAA GGCCATCGAG 
GCCCGGCACG CCTCCCTCAT GAAGAGGTGG AGCCAGCTTC TGGCCAACTC AGCCGCCCGC 
AAGAAGAAGC TTCTGGAGGC TCAGAGTCAC TTCCGCAAGG TGGAGGACCT CTTCCTGACC 
TTCGCCAAAA AGGCTTCTGC CTTCAACAGC TGGTTTGAAA ATGCAGAGGA GGACTTAACA 
GACCCCGTGC GCTGCAACTC CTTGGAAGAA ATCAAAGCTT TGCGCGAGGC CCACGACGCC 
TTCCGCTCCT CCCTCAGCTC TGCCCAGGCT GACTTCAACC AGCTGGCCGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT AGCCTCCAAC CCCTACACCT GGTTTACCAT GGAGGCCCTG 
GAGGAGACCT GGAGGAACCT ACAGAAAATC ATCAAGGAGA GGGAGCTGGA GCTGCAGAAG 
GAACAGCGGC GGCAGGAGGA GAACGACAAG CTGCGCCAGG AGTTTGCCCA GCACGCCAAC 
GCCTTCCACC AGTGGATCCA AGAGACCAGG TCCTGTATGG TGGAAGAGTC GGGGACCCTC 
GAATCCCAGC TTGAAGCTAC CAAACGCAAG CACCAGGAAA TCCGAGCCAT GAGAAGTCAG 
CTCAAAAAGA TCGAGGACCT GGGGGCCGCC ATGGAGGAGG CCCTCATCCT GGACAACAAG 
TACACGGAGC ACAGCACCGT GGGCCTCGCC CAGCAGTGGG ACCAGCTGGA CCAGCTGGGC 
ATGCGCATGC AGCACAACCT GGAGCAGCAG ATCCAGGCCA GGAACACAAC AGGTGTGACT 
GAGGAGGCCC TCAAAGAATT CAGCATGATG TTTAAACACT TTGACAAGGA CAAGTCTGGC 
AGGCTGAACC ATCAGGAGTT CAAATCTTGC CTGCGCTCCC TGGGCTATGA CCTGCCCATG 
GTGGAGGAAG GGGAACCTGA CCCTGAGTTC GAGGCAATCC TGGACACGGT GGATCCGAAC 
AGAGATGGCC ATGTCTCCTT GCAAGAATAC ATGGCTTTCA TGATCAGCCG CGAAACTGAG 
AACGTCAAGT CCAGCGAGGA GATTGAGAGC GCCTTCCGGG CCCTCAGCTC AGAGGGAAAG 
CCTTACGTGA CCAAGGAGGA GCTCTACCAG AACCTGACCC GGGAACAAGC CGACTACTGC 
GTCTCCCACA TGAAGCCCTA CGTGGACGGC AAGGGCCGCG AGCTCCCCAC CGCGTTCGAC 
TACGTGGAGT TCACCCGCTC GCTTTTCGTG AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001362242.1
CDS	114..7529
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELASDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMRRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPTDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRITKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGMMPRDETDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKQKLDILDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAAGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINGRFQKIKSMAASRRAKLNESHRLHQFFRDMDDEESWIKEKKLLVGSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHASLMKRWSQLLANSAARKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	NM_001375313.1
Organism	Homo sapiens(human)
Definition	Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant 11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001375313.1

ORF Insert Sequence:

ATGGACCCAA GTGGGGTCAA AGTGCTGGAA ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTAG ACCGATACCA CCGCTTCAAG GAACTCTCAA CCCTTAGGCG TCAGAAGCTG 
GAAGATTCCT ATCGATTCCA GTTCTTTCAA AGAGATGCTG AAGAGCTGGA GAAATGGATA 
CAGGAAAAAC TTCAGATTGC ATCTGATGAG AATTATAAAG ACCCAACCAA CTTGCAGGGA 
AAGCTTCAGA AGCATCAAGC ATTTGAAGCT GAAGTGCAGG CCAACTCAGG AGCCATTGTT 
AAGCTGGATG AAACTGGAAA CCTGATGATC TCAGAAGGGC ATTTTGCATC TGAAACCATA 
CGGACCCGTT TGATGGAGCT GCACCGCCAG TGGGAATTAC TTTTGGAGAA GATGCGAGAA 
AAAGGAATCA AACTGCTGCA GGCCCAGAAG TTGGTGCAGT ACTTACGAGA ATGTGAGGAC 
GTGATGGACT GGATCAATGA CAAGGAAGCA ATTGTTACTT CTGAAGAGCT GGGCCAGGAT 
CTGGAGCATG TAGAGGTTTT ACAGAAGAAA TTTGAAGAGT TTCAAACAGA TATGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTGAACCAG TTTGCTGCCA AACTCATACA GGAGCAGCAC 
CCTGAGGAGG AACTGATCAA GACTAAGCAG GATGAAGTCA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CTCTGCAGAG GCAGGGGAAG CTCTTTGGGG CAGCAGAAGT TCAGCGCTTT 
AACAGGGATG TGGATGAGAC TATCAGTTGG ATTAAGGAAA AGGAGCAGTT AATGGCCTCT 
GATGATTTTG GCCGAGACCT GGCAAGTGTT CAGGCTCTGC TTCGGAAGCA CGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAAGACAAG GTCAAAGCCC TGTGTGCTGA GGCTGACCGC 
CTGCAACAGT CCCACCCTCT GAGTGCAACA CAGATTCAAG TGAAGCGAGA GGAACTGATT 
ACAAACTGGG AGCAGATCCG CACCTTGGCG GCAGAGAGAC ATGCACGGCT CAATGATTCA 
TACAGGCTTC AACGCTTCCT TGCTGACTTC CGTGACCTCA CCAGCTGGGT GACTGAGATG 
AAAGCCCTCA TCAATGCAGA TGAGCTTGCC AGTGATGTGG CTGGGGCTGA AGCCCTGCTA 
GATAGACACC AAGAGCACAA GGGTGAAATT GATGCCCATG AAGACAGCTT CAAATCTGCA 
GATGAATCTG GACAGGCACT GCTTGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGACCG TCCTTTCCGA GGAGAGAGCG GCGCTGCTGG AGCTGTGGGA GCTGCGCAGG 
CAGCAGTACG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GGGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAATGAAG ACTTGGGAGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTTAA GAAGCACGAA GACTTTGAGA AATCCCTTAG TGCCCAGGAG 
GAAAAGATTA CAGCATTAGA TGAATTTGCA ACCAAGCTAA TTCAGAACAA CCACTATGCA 
ATGGAAGATG TGGCCACTCG CCGAGATGCT CTGTTGAGCC GCCGCAATGC CCTTCACGAG 
AGAGCCATGC GTCGCCGGGC CCAGCTAGCC GATTCTTTCC ATCTGCAGCA GTTTTTCCGT 
GATTCTGATG AGCTCAAGAG TTGGGTCAAT GAGAAGATGA AAACTGCCAC AGATGAAGCT 
TATAAAGATC CATCCAACCT ACAAGGAAAA GTACAGAAGC ATCAGGCTTT TGAGGCTGAG 
CTCTCAGCAA ACCAGAGCCG AATTGATGCC TTGGAGAAAG CTGGCCAAAA GCTGATTGAT 
GTCAACCACT ATGCCAAGGA TGAAGTGGCA GCTCGTATGA ATGAGGTGAT CAGTTTGTGG 
AAGAAACTGC TAGAGGCCAC TGAACTGAAA GGAATAAAGC TTCGTGAAGC CAACCAGCAA 
CAGCAATTTA ATCGCAATGT TGAGGATATT GAATTGTGGC TATATGAAGT AGAAGGTCAC 
TTGGCTTCGG ATGATTACGG CAAAGATCTT ACCAATGTGC AGAACCTCCA GAAGAAACAT 
GCACTGCTAG AGGCAGATGT GGCTGCTCAC CAGGACCGAA TTGATGGCAT CACCATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTT GATGCAGAAA ACATCAAGAA GAAACAGGAA 
GCCCTCGTGG CTCGCTATGA GGCACTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCCGATTCTC TGCGGTTGCA GCAGCTCTTC CGGGATGTTG AGGATGAGGA GACGTGGATT 
CGAGAGAAAG AGCCCATTGC CGCATCTACC AACAGAGGTA AGGATTTAAT TGGGGTCCAG 
AATCTGCTAA AGAAACATCA AGCCTTACAA GCAGAAATTG CTGGACATGA ACCACGCATC 
AAAGCAGTTA CACAGAAGGG GAATGCCATG GTGGAGGAAG GCCATTTTGC TGCAGAGGAT 
GTGAAGGCCA AGCTTCACGA GCTGAACCAA AAGTGGGAGG CACTGAAAGC CAAAGCTTCC 
CAGCGTCGGC AGGACCTGGA GGACTCTCTG CAGGCCCAGC AGTACTTTGC TGATGCTAAC 
GAGGCTGAAT CCTGGATGCG GGAGAAGGAA CCCATTGTGG GCAGCACTGA CTATGGCAAG 
GACGAAGACT CTGCTGAGGC TCTACTGAAG AAACACGAAG CTTTGATGTC AGATCTCAGT 
GCCTACGGCA GCAGCATCCA GGCTTTGCGA GAACAAGCAC AGTCCTGCCG GCAACAAGTG 
GCCCCCACGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CTCTCTACGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAG GGAGATATCC TTACCTTACT CAACAGCACC 
AACAAGGATT GGTGGAAAGT GGAAGTGAAC GATCGTCAGG GTTTTGTGCC GGCTGCGTAC 
GTGAAGAAAT TGGACCCCGC CCAGTCAGCC TCCCGGGAGA ATCTCCTGGA GGAGCAAGGC 
AGCATAGCAC TGCGGCAGGA GCAGATTGAC AATCAGACAC GCATAACTAA GGAGGCCGGC 
AGTGTATCTC TGCGTATGAA GCAGGTGGAA GAACTATATC ATTCTCTGCT GGAACTGGGT 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TTATGTTGTT CCGTGAAGCG 
AATGAACTAC AGCAATGGAT CAATGAGAAG GAAGCCGCTC TGACAAGTGA GGAGGTCGGA 
GCAGACTTGG AGCAGGTTGA GGTGCTCCAG AAGAAGTTTG ATGACTTCCA GAAGGACCTG 
AAGGCCAATG AGTCACGGTT GAAGGACATT AACAAGGTAG CTGAAGACCT GGAGTCTGAA 
GGTCTCATGG CAGAGGAGGT GCAGGCTGTG CAACAACAGG AAGTGTATGG CATGATGCCC 
AGGGATGAAA CTGATTCCAA GACAGCCTCC CCGTGGAAGT CTGCTCGTCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTACAGCAG 
CTGGCCGAGG AACGGAGCCA GCTCTTGGGC AGCGCCCATG AAGTACAGAG GTTCCACAGA 
GATGCTGATG AAACCAAAGA ATGGATTGAA GAGAAGAATC AAGCTCTAAA CACAGACAAT 
TATGGACATG ATCTCGCCAG TGTCCAGGCC CTGCAACGCA AGCATGAGGG CTTCGAGAGG 
GACCTTGCGG CTCTCGGTGA CAAGGTAAAC TCCCTTGGTG AAACAGCAGA GCGCCTGATC 
CAGTCCCATC CCGAGTCAGC AGAAGACCTG CAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGAGCAGCC TGGGGAAACG TGCAGATCAG CGCAAGGCAA AGTTGGGTGA CTCCCACGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGGGGG 
TTGGTGTCCT CAGATGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCATT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATCGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGACAGC AGCTGTTGGC TCACGGACAC TATGCCAGCC CTGAGATCAA GCAGAAACTT 
GATATTCTTG ACCAGGAGCG TGCAGACCTG GAGAAGGCCT GGGTTCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAACT GCAGCTGTTC CATCGGGACT GTGAGCAAGC TGAGAACTGG 
ATGGCTGCCC GGGAGGCCTT CTTGAATACC GAAGACAAAG GAGACTCACT GGACAGCGTA 
GAGGCTCTGA TCAAAAAACA TGAAGACTTT GACAAAGCGA TTAACGTCCA GGAAGAGAAG 
ATTGCTGCTC TGCAGGCCTT TGCCGACCAG CTCATCGCTG CCGGCCATTA TGCCAAGGGA 
GACATTTCTA GCCGGCGCAA TGAGGTCTTG GACAGGTGGC GACGTCTGAA AGCCCAGATG 
ATTGAGAAAA GGTCAAAGCT AGGAGAATCT CAAACCCTCC AACAGTTCAG CCGGGATGTG 
GATGAGATTG AGGCTTGGAT CAGTGAAAAA TTGCAAACAG CGAGTGATGA GTCGTACAAG 
GATCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ACCAGAAGCA CCAGGCTTTT 
GAAGCAGAGC TGCATGCCAA CGCTGACCGG ATCCGTGGGG TTATCGACAT GGGCAACTCC 
CTCATTGAAC GTGGAGCCTG TGCCGGCAGT GAGGATGCTG TCAAGGCCCG CCTGGCTGCC 
TTAGCTGACC AGTGGCAGTT CTTGGTGCAA AAGTCAGCGG AAAAGAGCCA GAAACTGAAA 
GAAGCCAACA AGCAGCAGAA CTTCAACACA GGGATCAAGG ACTTTGACTT CTGGCTGTCT 
GAGGTGGAGG CCCTGCTGGC ATCCGAAGAT TATGGCAAAG ACCTAGCTTC TGTGAACAAC 
CTGCTGAAAA AGCATCAACT GCTGGAAGCA GATATATCTG CCCATGAGGA TCGCCTGAAG 
GACCTGAACA GCCAGGCAGA CAGCCTGATG ACCAGCAGTG CCTTCGACAC CTCCCAAGTA 
AAGGACAAGA GGGACACCAT CAACGGGCGC TTCCAGAAGA TCAAGAGCAT GGCGGCCTCC 
CGGCGAGCCA AGCTGAATGA ATCCCATCGC CTGCACCAGT TCTTCCGGGA CATGGATGAC 
GAGGAGTCCT GGATCAAGGA GAAGAAGCTG CTGGTGGGCT CAGAGGACTA CGGCCGGGAC 
CTAACCGGCG TGCAGAACCT GAGGAAGAAG CACAAGCGGC TGGAAGCAGA ACTGGCTGCG 
CATGAGCCGG CTATTCAGGG TGTCCTGGAC ACTGGCAAGA AGCTGTCCGA TGACAACACC 
ATCGGGAAAG AGGAGATCCA GCAGCGGCTG GCGCAGTTTG TGGAGCACTG GAAAGAGCTG 
AAGCAGCTGG CAGCTGCCCG GGGTCAGCGG CTGGAAGAGT CCTTGGAATA TCAGCAGTTT 
GTAGCCAATG TGGAAGAGGA AGAAGCCTGG ATCAATGAGA AAATGACCCT GGTGGCCAGC 
GAAGATTATG GCGACACTCT TGCCGCCATC CAGGGCTTAC TGAAGAAACA TGAAGCTTTT 
GAGACAGACT TCACCGTCCA CAAGGATCGC GTGAATGATG TCTGCACCAA TGGACAAGAC 
CTCATTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CAAAGATGAA GGGCCTGAAC 
GGGAAAGTGT CAGACCTGGA GAAAGCTGCA GCCCAGAGAA AGGCGAAGCT GGATGAGAAC 
TCGGCCTTCC TTCAGTTCAA CTGGAAGGCG GACGTGGTGG AGTCCTGGAT CGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGC CGAGACCTGT CTTCTGTGCA GACGCTCCTC 
ACCAAACAGG AAACTTTTGA CGCTGGGCTG CAGGCCTTCC AGCAGGAAGG CATTGCCAAC 
ATCACTGCCC TCAAAGATCA GCTTCTCGCC GCCAAACACG TTCAGTCCAA GGCCATCGAG 
GCCCGGCACG CCTCCCTCAT GAAGAGGTGG AGCCAGCTTC TGGCCAACTC AGCCGCCCGC 
AAGAAGAAGC TTCTGGAGGC TCAGAGTCAC TTCCGCAAGG TGGAGGACCT CTTCCTGACC 
TTCGCCAAAA AGGCTTCTGC CTTCAACAGC TGGTTTGAAA ATGCAGAGGA GGACTTAACA 
GACCCCGTGC GCTGCAACTC CTTGGAAGAA ATCAAAGCTT TGCGCGAGGC CCACGACGCC 
TTCCGCTCCT CCCTCAGCTC TGCCCAGGCT GACTTCAACC AGCTGGCCGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT AGCCTCCAAC CCCTACACCT GGTTTACCAT GGAGGCCCTG 
GAGGAGACCT GGAGGAACCT ACAGAAAATC ATCAAGGAGA GGGAGCTGGA GCTGCAGAAG 
GAACAGCGGC GGCAGGAGGA GAACGACAAG CTGCGCCAGG AGTTTGCCCA GCACGCCAAC 
GCCTTCCACC AGTGGATCCA AGAGACCAGG TCCTGTATGG TGGAAGAGTC GGGGACCCTC 
GAATCCCAGC TTGAAGCTAC CAAACGCAAG CACCAGGAAA TCCGAGCCAT GAGAAGTCAG 
CTCAAAAAGA TCGAGGACCT GGGGGCCGCC ATGGAGGAGG CCCTCATCCT GGACAACAAG 
TACACGGAGC ACAGCACCGT GGGCCTCGCC CAGCAGTGGG ACCAGCTGGA CCAGCTGGGC 
ATGCGCATGC AGCACAACCT GGAGCAGCAG ATCCAGGCCA GGAACACAAC AGGTGTGACT 
GAGGAGGCCC TCAAAGAATT CAGCATGATG TTTAAACACT TTGACAAGGA CAAGTCTGGC 
AGGCTGAACC ATCAGGAGTT CAAATCTTGC CTGCGCTCCC TGGGCTATGA CCTGCCCATG 
GTGGAGGAAG GGGAACCTGA CCCTGAGTTC GAGGCAATCC TGGACACGGT GGATCCGAAC 
AGAGATGGCC ATGTCTCCTT GCAAGAATAC ATGGCTTTCA TGATCAGCCG CGAAACTGAG 
AACGTCAAGT CCAGCGAGGA GATTGAGAGC GCCTTCCGGG CCCTCAGCTC AGAGGGAAAG 
CCTTACGTGA CCAAGGAGGA GCTCTACCAG AACCTGACCC GGGAACAAGC CGACTACTGC 
GTCTCCCACA TGAAGCCCTA CGTGGACGGC AAGGGCCGCG AGCTCCCCAC CGCGTTCGAC 
TACGTGGAGT TCACCCGCTC GCTTTTCGTG AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( NM_001363765.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001350694.1 Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant 5, mRNA.

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CloneID	OHu98857
Clone ID Related Accession (Same CDS sequence)	NM_001363765.2 , NM_001375314.2 , XM_017015061.1 , NM_001363765.1
Accession Version	NM_001363765.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	spectrin alpha chain, non-erythrocytic 1 isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL356481.16. On Jun 2, 2019 this sequence version replaced NM_001363765.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.249773.1, SRR1803612.276815.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGGACCCAA GTGGGGTCAA AGTGCTGGAA ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTAG ACCGATACCA CCGCTTCAAG GAACTCTCAA CCCTTAGGCG TCAGAAGCTG 
GAAGATTCCT ATCGATTCCA GTTCTTTCAA AGAGATGCTG AAGAGCTGGA GAAATGGATA 
CAGGAAAAAC TTCAGATTGC ATCTGATGAG AATTATAAAG ACCCAACCAA CTTGCAGGGA 
AAGCTTCAGA AGCATCAAGC ATTTGAAGCT GAAGTGCAGG CCAACTCAGG AGCCATTGTT 
AAGCTGGATG AAACTGGAAA CCTGATGATC TCAGAAGGGC ATTTTGCATC TGAAACCATA 
CGGACCCGTT TGATGGAGCT GCACCGCCAG TGGGAATTAC TTTTGGAGAA GATGCGAGAA 
AAAGGAATCA AACTGCTGCA GGCCCAGAAG TTGGTGCAGT ACTTACGAGA ATGTGAGGAC 
GTGATGGACT GGATCAATGA CAAGGAAGCA ATTGTTACTT CTGAAGAGCT GGGCCAGGAT 
CTGGAGCATG TAGAGGTTTT ACAGAAGAAA TTTGAAGAGT TTCAAACAGA TATGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTGAACCAG TTTGCTGCCA AACTCATACA GGAGCAGCAC 
CCTGAGGAGG AACTGATCAA GACTAAGCAG GATGAAGTCA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CTCTGCAGAG GCAGGGGAAG CTCTTTGGGG CAGCAGAAGT TCAGCGCTTT 
AACAGGGATG TGGATGAGAC TATCAGTTGG ATTAAGGAAA AGGAGCAGTT AATGGCCTCT 
GATGATTTTG GCCGAGACCT GGCAAGTGTT CAGGCTCTGC TTCGGAAGCA CGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAAGACAAG GTCAAAGCCC TGTGTGCTGA GGCTGACCGC 
CTGCAACAGT CCCACCCTCT GAGTGCAACA CAGATTCAAG TGAAGCGAGA GGAACTGATT 
ACAAACTGGG AGCAGATCCG CACCTTGGCG GCAGAGAGAC ATGCACGGCT CAATGATTCA 
TACAGGCTTC AACGCTTCCT TGCTGACTTC CGTGACCTCA CCAGCTGGGT GACTGAGATG 
AAAGCCCTCA TCAATGCAGA TGAGCTTGCC AGTGATGTGG CTGGGGCTGA AGCCCTGCTA 
GATAGACACC AAGAGCACAA GGGTGAAATT GATGCCCATG AAGACAGCTT CAAATCTGCA 
GATGAATCTG GACAGGCACT GCTTGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGACCG TCCTTTCCGA GGAGAGAGCG GCGCTGCTGG AGCTGTGGGA GCTGCGCAGG 
CAGCAGTACG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GGGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAATGAAG ACTTGGGAGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTTAA GAAGCACGAA GACTTTGAGA AATCCCTTAG TGCCCAGGAG 
GAAAAGATTA CAGCATTAGA TGAATTTGCA ACCAAGCTAA TTCAGAACAA CCACTATGCA 
ATGGAAGATG TGGCCACTCG CCGAGATGCT CTGTTGAGCC GCCGCAATGC CCTTCACGAG 
AGAGCCATGC GTCGCCGGGC CCAGCTAGCC GATTCTTTCC ATCTGCAGCA GTTTTTCCGT 
GATTCTGATG AGCTCAAGAG TTGGGTCAAT GAGAAGATGA AAACTGCCAC AGATGAAGCT 
TATAAAGATC CATCCAACCT ACAAGGAAAA GTACAGAAGC ATCAGGCTTT TGAGGCTGAG 
CTCTCAGCAA ACCAGAGCCG AATTGATGCC TTGGAGAAAG CTGGCCAAAA GCTGATTGAT 
GTCAACCACT ATGCCAAGGA TGAAGTGGCA GCTCGTATGA ATGAGGTGAT CAGTTTGTGG 
AAGAAACTGC TAGAGGCCAC TGAACTGAAA GGAATAAAGC TTCGTGAAGC CAACCAGCAA 
CAGCAATTTA ATCGCAATGT TGAGGATATT GAATTGTGGC TATATGAAGT AGAAGGTCAC 
TTGGCTTCGG ATGATTACGG CAAAGATCTT ACCAATGTGC AGAACCTCCA GAAGAAACAT 
GCACTGCTAG AGGCAGATGT GGCTGCTCAC CAGGACCGAA TTGATGGCAT CACCATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTT GATGCAGAAA ACATCAAGAA GAAACAGGAA 
GCCCTCGTGG CTCGCTATGA GGCACTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCCGATTCTC TGCGGTTGCA GCAGCTCTTC CGGGATGTTG AGGATGAGGA GACGTGGATT 
CGAGAGAAAG AGCCCATTGC CGCATCTACC AACAGAGGTA AGGATTTAAT TGGGGTCCAG 
AATCTGCTAA AGAAACATCA AGCCTTACAA GCAGAAATTG CTGGACATGA ACCACGCATC 
AAAGCAGTTA CACAGAAGGG GAATGCCATG GTGGAGGAAG GCCATTTTGC TGCAGAGGAT 
GTGAAGGCCA AGCTTCACGA GCTGAACCAA AAGTGGGAGG CACTGAAAGC CAAAGCTTCC 
CAGCGTCGGC AGGACCTGGA GGACTCTCTG CAGGCCCAGC AGTACTTTGC TGATGCTAAC 
GAGGCTGAAT CCTGGATGCG GGAGAAGGAA CCCATTGTGG GCAGCACTGA CTATGGCAAG 
GACGAAGACT CTGCTGAGGC TCTACTGAAG AAACACGAAG CTTTGATGTC AGATCTCAGT 
GCCTACGGCA GCAGCATCCA GGCTTTGCGA GAACAAGCAC AGTCCTGCCG GCAACAAGTG 
GCCCCCACGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CTCTCTACGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAG GGAGATATCC TTACCTTACT CAACAGCACC 
AACAAGGATT GGTGGAAAGT GGAAGTGAAC GATCGTCAGG GTTTTGTGCC GGCTGCGTAC 
GTGAAGAAAT TGGACCCCGC CCAGTCAGCC TCCCGGGAGA ATCTCCTGGA GGAGCAAGGC 
AGCATAGCAC TGCGGCAGGA GCAGATTGAC AATCAATATC ATTCTCTGCT GGAACTGGGT 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TTATGTTGTT CCGTGAAGCG 
AATGAACTAC AGCAATGGAT CAATGAGAAG GAAGCCGCTC TGACAAGTGA GGAGGTCGGA 
GCAGACTTGG AGCAGGTTGA GGTGCTCCAG AAGAAGTTTG ATGACTTCCA GAAGGACCTG 
AAGGCCAATG AGTCACGGTT GAAGGACATT AACAAGGTAG CTGAAGACCT GGAGTCTGAA 
GGTCTCATGG CAGAGGAGGT GCAGGCTGTG CAACAACAGG AAGTGTATGG CATGATGCCC 
AGGGATGAAA CTGATTCCAA GACAGCCTCC CCGTGGAAGT CTGCTCGTCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTACAGCAG 
CTGGCCGAGG AACGGAGCCA GCTCTTGGGC AGCGCCCATG AAGTACAGAG GTTCCACAGA 
GATGCTGATG AAACCAAAGA ATGGATTGAA GAGAAGAATC AAGCTCTAAA CACAGACAAT 
TATGGACATG ATCTCGCCAG TGTCCAGGCC CTGCAACGCA AGCATGAGGG CTTCGAGAGG 
GACCTTGCGG CTCTCGGTGA CAAGGTAAAC TCCCTTGGTG AAACAGCAGA GCGCCTGATC 
CAGTCCCATC CCGAGTCAGC AGAAGACCTG CAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGAGCAGCC TGGGGAAACG TGCAGATCAG CGCAAGGCAA AGTTGGGTGA CTCCCACGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGGGGG 
TTGGTGTCCT CAGATGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCATT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATCGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGACAGC AGCTGTTGGC TCACGGACAC TATGCCAGCC CTGAGATCAA GCAGAAACTT 
GATATTCTTG ACCAGGAGCG TGCAGACCTG GAGAAGGCCT GGGTTCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAACT GCAGCTGTTC CATCGGGACT GTGAGCAAGC TGAGAACTGG 
ATGGCTGCCC GGGAGGCCTT CTTGAATACC GAAGACAAAG GAGACTCACT GGACAGCGTA 
GAGGCTCTGA TCAAAAAACA TGAAGACTTT GACAAAGCGA TTAACGTCCA GGAAGAGAAG 
ATTGCTGCTC TGCAGGCCTT TGCCGACCAG CTCATCGCTG CCGGCCATTA TGCCAAGGGA 
GACATTTCTA GCCGGCGCAA TGAGGTCTTG GACAGGTGGC GACGTCTGAA AGCCCAGATG 
ATTGAGAAAA GGTCAAAGCT AGGAGAATCT CAAACCCTCC AACAGTTCAG CCGGGATGTG 
GATGAGATTG AGGCTTGGAT CAGTGAAAAA TTGCAAACAG CGAGTGATGA GTCGTACAAG 
GATCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ACCAGAAGCA CCAGGCTTTT 
GAAGCAGAGC TGCATGCCAA CGCTGACCGG ATCCGTGGGG TTATCGACAT GGGCAACTCC 
CTCATTGAAC GTGGAGCCTG TGCCGGCAGT GAGGATGCTG TCAAGGCCCG CCTGGCTGCC 
TTAGCTGACC AGTGGCAGTT CTTGGTGCAA AAGTCAGCGG AAAAGAGCCA GAAACTGAAA 
GAAGCCAACA AGCAGCAGAA CTTCAACACA GGGATCAAGG ACTTTGACTT CTGGCTGTCT 
GAGGTGGAGG CCCTGCTGGC ATCCGAAGAT TATGGCAAAG ACCTAGCTTC TGTGAACAAC 
CTGCTGAAAA AGCATCAACT GCTGGAAGCA GATATATCTG CCCATGAGGA TCGCCTGAAG 
GACCTGAACA GCCAGGCAGA CAGCCTGATG ACCAGCAGTG CCTTCGACAC CTCCCAAGTA 
AAGGACAAGA GGGACACCAT CAACGGGCGC TTCCAGAAGA TCAAGAGCAT GGCGGCCTCC 
CGGCGAGCCA AGCTGAATGA ATCCCATCGC CTGCACCAGT TCTTCCGGGA CATGGATGAC 
GAGGAGTCCT GGATCAAGGA GAAGAAGCTG CTGGTGGGCT CAGAGGACTA CGGCCGGGAC 
CTAACCGGCG TGCAGAACCT GAGGAAGAAG CACAAGCGGC TGGAAGCAGA ACTGGCTGCG 
CATGAGCCGG CTATTCAGGG TGTCCTGGAC ACTGGCAAGA AGCTGTCCGA TGACAACACC 
ATCGGGAAAG AGGAGATCCA GCAGCGGCTG GCGCAGTTTG TGGAGCACTG GAAAGAGCTG 
AAGCAGCTGG CAGCTGCCCG GGGTCAGCGG CTGGAAGAGT CCTTGGAATA TCAGCAGTTT 
GTAGCCAATG TGGAAGAGGA AGAAGCCTGG ATCAATGAGA AAATGACCCT GGTGGCCAGC 
GAAGATTATG GCGACACTCT TGCCGCCATC CAGGGCTTAC TGAAGAAACA TGAAGCTTTT 
GAGACAGACT TCACCGTCCA CAAGGATCGC GTGAATGATG TCTGCACCAA TGGACAAGAC 
CTCATTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CAAAGATGAA GGGCCTGAAC 
GGGAAAGTGT CAGACCTGGA GAAAGCTGCA GCCCAGAGAA AGGCGAAGCT GGATGAGAAC 
TCGGCCTTCC TTCAGTTCAA CTGGAAGGCG GACGTGGTGG AGTCCTGGAT CGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGC CGAGACCTGT CTTCTGTGCA GACGCTCCTC 
ACCAAACAGG AAACTTTTGA CGCTGGGCTG CAGGCCTTCC AGCAGGAAGG CATTGCCAAC 
ATCACTGCCC TCAAAGATCA GCTTCTCGCC GCCAAACACG TTCAGTCCAA GGCCATCGAG 
GCCCGGCACG CCTCCCTCAT GAAGAGGTGG AGCCAGCTTC TGGCCAACTC AGCCGCCCGC 
AAGAAGAAGC TTCTGGAGGC TCAGAGTCAC TTCCGCAAGG TGGAGGACCT CTTCCTGACC 
TTCGCCAAAA AGGCTTCTGC CTTCAACAGC TGGTTTGAAA ATGCAGAGGA GGACTTAACA 
GACCCCGTGC GCTGCAACTC CTTGGAAGAA ATCAAAGCTT TGCGCGAGGC CCACGACGCC 
TTCCGCTCCT CCCTCAGCTC TGCCCAGGCT GACTTCAACC AGCTGGCCGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT AGCCTCCAAC CCCTACACCT GGTTTACCAT GGAGGCCCTG 
GAGGAGACCT GGAGGAACCT ACAGAAAATC ATCAAGGAGA GGGAGCTGGA GCTGCAGAAG 
GAACAGCGGC GGCAGGAGGA GAACGACAAG CTGCGCCAGG AGTTTGCCCA GCACGCCAAC 
GCCTTCCACC AGTGGATCCA AGAGACCAGG ACATACCTCC TCGATGGGTC CTGTATGGTG 
GAAGAGTCGG GGACCCTCGA ATCCCAGCTT GAAGCTACCA AACGCAAGCA CCAGGAAATC 
CGAGCCATGA GAAGTCAGCT CAAAAAGATC GAGGACCTGG GGGCCGCCAT GGAGGAGGCC 
CTCATCCTGG ACAACAAGTA CACGGAGCAC AGCACCGTGG GCCTCGCCCA GCAGTGGGAC 
CAGCTGGACC AGCTGGGCAT GCGCATGCAG CACAACCTGG AGCAGCAGAT CCAGGCCAGG 
AACACAACAG GTGTGACTGA GGAGGCCCTC AAAGAATTCA GCATGATGTT TAAACACTTT 
GACAAGGACA AGTCTGGCAG GCTGAACCAT CAGGAGTTCA AATCTTGCCT GCGCTCCCTG 
GGCTATGACC TGCCCATGGT GGAGGAAGGG GAACCTGACC CTGAGTTCGA GGCAATCCTG 
GACACGGTGG ATCCGAACAG AGATGGCCAT GTCTCCTTGC AAGAATACAT GGCTTTCATG 
ATCAGCCGCG AAACTGAGAA CGTCAAGTCC AGCGAGGAGA TTGAGAGCGC CTTCCGGGCC 
CTCAGCTCAG AGGGAAAGCC TTACGTGACC AAGGAGGAGC TCTACCAGAA CCTGACCCGG 
GAACAAGCCG ACTACTGCGT CTCCCACATG AAGCCCTACG TGGACGGCAA GGGCCGCGAG 
CTCCCCACCG CGTTCGACTA CGTGGAGTTC ACCCGCTCGC TTTTCGTGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350694.1
CDS	114..7487
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELASDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMRRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPTDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGMMPRDETDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKQKLDILDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAAGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINGRFQKIKSMAASRRAKLNESHRLHQFFRDMDDEESWIKEKKLLVGSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHASLMKRWSQLLANSAARKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	NM_001363765.2
Organism	Homo sapiens(human)
Definition	Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363765.2

ORF Insert Sequence:

ATGGACCCAA GTGGGGTCAA AGTGCTGGAA ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTAG ACCGATACCA CCGCTTCAAG GAACTCTCAA CCCTTAGGCG TCAGAAGCTG 
GAAGATTCCT ATCGATTCCA GTTCTTTCAA AGAGATGCTG AAGAGCTGGA GAAATGGATA 
CAGGAAAAAC TTCAGATTGC ATCTGATGAG AATTATAAAG ACCCAACCAA CTTGCAGGGA 
AAGCTTCAGA AGCATCAAGC ATTTGAAGCT GAAGTGCAGG CCAACTCAGG AGCCATTGTT 
AAGCTGGATG AAACTGGAAA CCTGATGATC TCAGAAGGGC ATTTTGCATC TGAAACCATA 
CGGACCCGTT TGATGGAGCT GCACCGCCAG TGGGAATTAC TTTTGGAGAA GATGCGAGAA 
AAAGGAATCA AACTGCTGCA GGCCCAGAAG TTGGTGCAGT ACTTACGAGA ATGTGAGGAC 
GTGATGGACT GGATCAATGA CAAGGAAGCA ATTGTTACTT CTGAAGAGCT GGGCCAGGAT 
CTGGAGCATG TAGAGGTTTT ACAGAAGAAA TTTGAAGAGT TTCAAACAGA TATGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTGAACCAG TTTGCTGCCA AACTCATACA GGAGCAGCAC 
CCTGAGGAGG AACTGATCAA GACTAAGCAG GATGAAGTCA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CTCTGCAGAG GCAGGGGAAG CTCTTTGGGG CAGCAGAAGT TCAGCGCTTT 
AACAGGGATG TGGATGAGAC TATCAGTTGG ATTAAGGAAA AGGAGCAGTT AATGGCCTCT 
GATGATTTTG GCCGAGACCT GGCAAGTGTT CAGGCTCTGC TTCGGAAGCA CGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAAGACAAG GTCAAAGCCC TGTGTGCTGA GGCTGACCGC 
CTGCAACAGT CCCACCCTCT GAGTGCAACA CAGATTCAAG TGAAGCGAGA GGAACTGATT 
ACAAACTGGG AGCAGATCCG CACCTTGGCG GCAGAGAGAC ATGCACGGCT CAATGATTCA 
TACAGGCTTC AACGCTTCCT TGCTGACTTC CGTGACCTCA CCAGCTGGGT GACTGAGATG 
AAAGCCCTCA TCAATGCAGA TGAGCTTGCC AGTGATGTGG CTGGGGCTGA AGCCCTGCTA 
GATAGACACC AAGAGCACAA GGGTGAAATT GATGCCCATG AAGACAGCTT CAAATCTGCA 
GATGAATCTG GACAGGCACT GCTTGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGACCG TCCTTTCCGA GGAGAGAGCG GCGCTGCTGG AGCTGTGGGA GCTGCGCAGG 
CAGCAGTACG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GGGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAATGAAG ACTTGGGAGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTTAA GAAGCACGAA GACTTTGAGA AATCCCTTAG TGCCCAGGAG 
GAAAAGATTA CAGCATTAGA TGAATTTGCA ACCAAGCTAA TTCAGAACAA CCACTATGCA 
ATGGAAGATG TGGCCACTCG CCGAGATGCT CTGTTGAGCC GCCGCAATGC CCTTCACGAG 
AGAGCCATGC GTCGCCGGGC CCAGCTAGCC GATTCTTTCC ATCTGCAGCA GTTTTTCCGT 
GATTCTGATG AGCTCAAGAG TTGGGTCAAT GAGAAGATGA AAACTGCCAC AGATGAAGCT 
TATAAAGATC CATCCAACCT ACAAGGAAAA GTACAGAAGC ATCAGGCTTT TGAGGCTGAG 
CTCTCAGCAA ACCAGAGCCG AATTGATGCC TTGGAGAAAG CTGGCCAAAA GCTGATTGAT 
GTCAACCACT ATGCCAAGGA TGAAGTGGCA GCTCGTATGA ATGAGGTGAT CAGTTTGTGG 
AAGAAACTGC TAGAGGCCAC TGAACTGAAA GGAATAAAGC TTCGTGAAGC CAACCAGCAA 
CAGCAATTTA ATCGCAATGT TGAGGATATT GAATTGTGGC TATATGAAGT AGAAGGTCAC 
TTGGCTTCGG ATGATTACGG CAAAGATCTT ACCAATGTGC AGAACCTCCA GAAGAAACAT 
GCACTGCTAG AGGCAGATGT GGCTGCTCAC CAGGACCGAA TTGATGGCAT CACCATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTT GATGCAGAAA ACATCAAGAA GAAACAGGAA 
GCCCTCGTGG CTCGCTATGA GGCACTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCCGATTCTC TGCGGTTGCA GCAGCTCTTC CGGGATGTTG AGGATGAGGA GACGTGGATT 
CGAGAGAAAG AGCCCATTGC CGCATCTACC AACAGAGGTA AGGATTTAAT TGGGGTCCAG 
AATCTGCTAA AGAAACATCA AGCCTTACAA GCAGAAATTG CTGGACATGA ACCACGCATC 
AAAGCAGTTA CACAGAAGGG GAATGCCATG GTGGAGGAAG GCCATTTTGC TGCAGAGGAT 
GTGAAGGCCA AGCTTCACGA GCTGAACCAA AAGTGGGAGG CACTGAAAGC CAAAGCTTCC 
CAGCGTCGGC AGGACCTGGA GGACTCTCTG CAGGCCCAGC AGTACTTTGC TGATGCTAAC 
GAGGCTGAAT CCTGGATGCG GGAGAAGGAA CCCATTGTGG GCAGCACTGA CTATGGCAAG 
GACGAAGACT CTGCTGAGGC TCTACTGAAG AAACACGAAG CTTTGATGTC AGATCTCAGT 
GCCTACGGCA GCAGCATCCA GGCTTTGCGA GAACAAGCAC AGTCCTGCCG GCAACAAGTG 
GCCCCCACGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CTCTCTACGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAG GGAGATATCC TTACCTTACT CAACAGCACC 
AACAAGGATT GGTGGAAAGT GGAAGTGAAC GATCGTCAGG GTTTTGTGCC GGCTGCGTAC 
GTGAAGAAAT TGGACCCCGC CCAGTCAGCC TCCCGGGAGA ATCTCCTGGA GGAGCAAGGC 
AGCATAGCAC TGCGGCAGGA GCAGATTGAC AATCAATATC ATTCTCTGCT GGAACTGGGT 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TTATGTTGTT CCGTGAAGCG 
AATGAACTAC AGCAATGGAT CAATGAGAAG GAAGCCGCTC TGACAAGTGA GGAGGTCGGA 
GCAGACTTGG AGCAGGTTGA GGTGCTCCAG AAGAAGTTTG ATGACTTCCA GAAGGACCTG 
AAGGCCAATG AGTCACGGTT GAAGGACATT AACAAGGTAG CTGAAGACCT GGAGTCTGAA 
GGTCTCATGG CAGAGGAGGT GCAGGCTGTG CAACAACAGG AAGTGTATGG CATGATGCCC 
AGGGATGAAA CTGATTCCAA GACAGCCTCC CCGTGGAAGT CTGCTCGTCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTACAGCAG 
CTGGCCGAGG AACGGAGCCA GCTCTTGGGC AGCGCCCATG AAGTACAGAG GTTCCACAGA 
GATGCTGATG AAACCAAAGA ATGGATTGAA GAGAAGAATC AAGCTCTAAA CACAGACAAT 
TATGGACATG ATCTCGCCAG TGTCCAGGCC CTGCAACGCA AGCATGAGGG CTTCGAGAGG 
GACCTTGCGG CTCTCGGTGA CAAGGTAAAC TCCCTTGGTG AAACAGCAGA GCGCCTGATC 
CAGTCCCATC CCGAGTCAGC AGAAGACCTG CAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGAGCAGCC TGGGGAAACG TGCAGATCAG CGCAAGGCAA AGTTGGGTGA CTCCCACGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGGGGG 
TTGGTGTCCT CAGATGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCATT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATCGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGACAGC AGCTGTTGGC TCACGGACAC TATGCCAGCC CTGAGATCAA GCAGAAACTT 
GATATTCTTG ACCAGGAGCG TGCAGACCTG GAGAAGGCCT GGGTTCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAACT GCAGCTGTTC CATCGGGACT GTGAGCAAGC TGAGAACTGG 
ATGGCTGCCC GGGAGGCCTT CTTGAATACC GAAGACAAAG GAGACTCACT GGACAGCGTA 
GAGGCTCTGA TCAAAAAACA TGAAGACTTT GACAAAGCGA TTAACGTCCA GGAAGAGAAG 
ATTGCTGCTC TGCAGGCCTT TGCCGACCAG CTCATCGCTG CCGGCCATTA TGCCAAGGGA 
GACATTTCTA GCCGGCGCAA TGAGGTCTTG GACAGGTGGC GACGTCTGAA AGCCCAGATG 
ATTGAGAAAA GGTCAAAGCT AGGAGAATCT CAAACCCTCC AACAGTTCAG CCGGGATGTG 
GATGAGATTG AGGCTTGGAT CAGTGAAAAA TTGCAAACAG CGAGTGATGA GTCGTACAAG 
GATCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ACCAGAAGCA CCAGGCTTTT 
GAAGCAGAGC TGCATGCCAA CGCTGACCGG ATCCGTGGGG TTATCGACAT GGGCAACTCC 
CTCATTGAAC GTGGAGCCTG TGCCGGCAGT GAGGATGCTG TCAAGGCCCG CCTGGCTGCC 
TTAGCTGACC AGTGGCAGTT CTTGGTGCAA AAGTCAGCGG AAAAGAGCCA GAAACTGAAA 
GAAGCCAACA AGCAGCAGAA CTTCAACACA GGGATCAAGG ACTTTGACTT CTGGCTGTCT 
GAGGTGGAGG CCCTGCTGGC ATCCGAAGAT TATGGCAAAG ACCTAGCTTC TGTGAACAAC 
CTGCTGAAAA AGCATCAACT GCTGGAAGCA GATATATCTG CCCATGAGGA TCGCCTGAAG 
GACCTGAACA GCCAGGCAGA CAGCCTGATG ACCAGCAGTG CCTTCGACAC CTCCCAAGTA 
AAGGACAAGA GGGACACCAT CAACGGGCGC TTCCAGAAGA TCAAGAGCAT GGCGGCCTCC 
CGGCGAGCCA AGCTGAATGA ATCCCATCGC CTGCACCAGT TCTTCCGGGA CATGGATGAC 
GAGGAGTCCT GGATCAAGGA GAAGAAGCTG CTGGTGGGCT CAGAGGACTA CGGCCGGGAC 
CTAACCGGCG TGCAGAACCT GAGGAAGAAG CACAAGCGGC TGGAAGCAGA ACTGGCTGCG 
CATGAGCCGG CTATTCAGGG TGTCCTGGAC ACTGGCAAGA AGCTGTCCGA TGACAACACC 
ATCGGGAAAG AGGAGATCCA GCAGCGGCTG GCGCAGTTTG TGGAGCACTG GAAAGAGCTG 
AAGCAGCTGG CAGCTGCCCG GGGTCAGCGG CTGGAAGAGT CCTTGGAATA TCAGCAGTTT 
GTAGCCAATG TGGAAGAGGA AGAAGCCTGG ATCAATGAGA AAATGACCCT GGTGGCCAGC 
GAAGATTATG GCGACACTCT TGCCGCCATC CAGGGCTTAC TGAAGAAACA TGAAGCTTTT 
GAGACAGACT TCACCGTCCA CAAGGATCGC GTGAATGATG TCTGCACCAA TGGACAAGAC 
CTCATTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CAAAGATGAA GGGCCTGAAC 
GGGAAAGTGT CAGACCTGGA GAAAGCTGCA GCCCAGAGAA AGGCGAAGCT GGATGAGAAC 
TCGGCCTTCC TTCAGTTCAA CTGGAAGGCG GACGTGGTGG AGTCCTGGAT CGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGC CGAGACCTGT CTTCTGTGCA GACGCTCCTC 
ACCAAACAGG AAACTTTTGA CGCTGGGCTG CAGGCCTTCC AGCAGGAAGG CATTGCCAAC 
ATCACTGCCC TCAAAGATCA GCTTCTCGCC GCCAAACACG TTCAGTCCAA GGCCATCGAG 
GCCCGGCACG CCTCCCTCAT GAAGAGGTGG AGCCAGCTTC TGGCCAACTC AGCCGCCCGC 
AAGAAGAAGC TTCTGGAGGC TCAGAGTCAC TTCCGCAAGG TGGAGGACCT CTTCCTGACC 
TTCGCCAAAA AGGCTTCTGC CTTCAACAGC TGGTTTGAAA ATGCAGAGGA GGACTTAACA 
GACCCCGTGC GCTGCAACTC CTTGGAAGAA ATCAAAGCTT TGCGCGAGGC CCACGACGCC 
TTCCGCTCCT CCCTCAGCTC TGCCCAGGCT GACTTCAACC AGCTGGCCGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT AGCCTCCAAC CCCTACACCT GGTTTACCAT GGAGGCCCTG 
GAGGAGACCT GGAGGAACCT ACAGAAAATC ATCAAGGAGA GGGAGCTGGA GCTGCAGAAG 
GAACAGCGGC GGCAGGAGGA GAACGACAAG CTGCGCCAGG AGTTTGCCCA GCACGCCAAC 
GCCTTCCACC AGTGGATCCA AGAGACCAGG ACATACCTCC TCGATGGGTC CTGTATGGTG 
GAAGAGTCGG GGACCCTCGA ATCCCAGCTT GAAGCTACCA AACGCAAGCA CCAGGAAATC 
CGAGCCATGA GAAGTCAGCT CAAAAAGATC GAGGACCTGG GGGCCGCCAT GGAGGAGGCC 
CTCATCCTGG ACAACAAGTA CACGGAGCAC AGCACCGTGG GCCTCGCCCA GCAGTGGGAC 
CAGCTGGACC AGCTGGGCAT GCGCATGCAG CACAACCTGG AGCAGCAGAT CCAGGCCAGG 
AACACAACAG GTGTGACTGA GGAGGCCCTC AAAGAATTCA GCATGATGTT TAAACACTTT 
GACAAGGACA AGTCTGGCAG GCTGAACCAT CAGGAGTTCA AATCTTGCCT GCGCTCCCTG 
GGCTATGACC TGCCCATGGT GGAGGAAGGG GAACCTGACC CTGAGTTCGA GGCAATCCTG 
GACACGGTGG ATCCGAACAG AGATGGCCAT GTCTCCTTGC AAGAATACAT GGCTTTCATG 
ATCAGCCGCG AAACTGAGAA CGTCAAGTCC AGCGAGGAGA TTGAGAGCGC CTTCCGGGCC 
CTCAGCTCAG AGGGAAAGCC TTACGTGACC AAGGAGGAGC TCTACCAGAA CCTGACCCGG 
GAACAAGCCG ACTACTGCGT CTCCCACATG AAGCCCTACG TGGACGGCAA GGGCCGCGAG 
CTCCCCACCG CGTTCGACTA CGTGGAGTTC ACCCGCTCGC TTTTCGTGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( NM_001375314.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001362243.1 Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant 10, mRNA.

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CloneID	OHu98857
Clone ID Related Accession (Same CDS sequence)	NM_001363765.2 , NM_001375314.2 , XM_017015061.1 , NM_001363765.1
Accession Version	NM_001375314.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	spectrin alpha chain, non-erythrocytic 1 isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL356481.16. On Oct 23, 2019 this sequence version replaced NM_001375314.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: SRR1803614.209499.1 [ECO:0000331] ##Evidence-Data-END##

ATGGACCCAA GTGGGGTCAA AGTGCTGGAA ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTAG ACCGATACCA CCGCTTCAAG GAACTCTCAA CCCTTAGGCG TCAGAAGCTG 
GAAGATTCCT ATCGATTCCA GTTCTTTCAA AGAGATGCTG AAGAGCTGGA GAAATGGATA 
CAGGAAAAAC TTCAGATTGC ATCTGATGAG AATTATAAAG ACCCAACCAA CTTGCAGGGA 
AAGCTTCAGA AGCATCAAGC ATTTGAAGCT GAAGTGCAGG CCAACTCAGG AGCCATTGTT 
AAGCTGGATG AAACTGGAAA CCTGATGATC TCAGAAGGGC ATTTTGCATC TGAAACCATA 
CGGACCCGTT TGATGGAGCT GCACCGCCAG TGGGAATTAC TTTTGGAGAA GATGCGAGAA 
AAAGGAATCA AACTGCTGCA GGCCCAGAAG TTGGTGCAGT ACTTACGAGA ATGTGAGGAC 
GTGATGGACT GGATCAATGA CAAGGAAGCA ATTGTTACTT CTGAAGAGCT GGGCCAGGAT 
CTGGAGCATG TAGAGGTTTT ACAGAAGAAA TTTGAAGAGT TTCAAACAGA TATGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTGAACCAG TTTGCTGCCA AACTCATACA GGAGCAGCAC 
CCTGAGGAGG AACTGATCAA GACTAAGCAG GATGAAGTCA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CTCTGCAGAG GCAGGGGAAG CTCTTTGGGG CAGCAGAAGT TCAGCGCTTT 
AACAGGGATG TGGATGAGAC TATCAGTTGG ATTAAGGAAA AGGAGCAGTT AATGGCCTCT 
GATGATTTTG GCCGAGACCT GGCAAGTGTT CAGGCTCTGC TTCGGAAGCA CGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAAGACAAG GTCAAAGCCC TGTGTGCTGA GGCTGACCGC 
CTGCAACAGT CCCACCCTCT GAGTGCAACA CAGATTCAAG TGAAGCGAGA GGAACTGATT 
ACAAACTGGG AGCAGATCCG CACCTTGGCG GCAGAGAGAC ATGCACGGCT CAATGATTCA 
TACAGGCTTC AACGCTTCCT TGCTGACTTC CGTGACCTCA CCAGCTGGGT GACTGAGATG 
AAAGCCCTCA TCAATGCAGA TGAGCTTGCC AGTGATGTGG CTGGGGCTGA AGCCCTGCTA 
GATAGACACC AAGAGCACAA GGGTGAAATT GATGCCCATG AAGACAGCTT CAAATCTGCA 
GATGAATCTG GACAGGCACT GCTTGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGACCG TCCTTTCCGA GGAGAGAGCG GCGCTGCTGG AGCTGTGGGA GCTGCGCAGG 
CAGCAGTACG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GGGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAATGAAG ACTTGGGAGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTTAA GAAGCACGAA GACTTTGAGA AATCCCTTAG TGCCCAGGAG 
GAAAAGATTA CAGCATTAGA TGAATTTGCA ACCAAGCTAA TTCAGAACAA CCACTATGCA 
ATGGAAGATG TGGCCACTCG CCGAGATGCT CTGTTGAGCC GCCGCAATGC CCTTCACGAG 
AGAGCCATGC GTCGCCGGGC CCAGCTAGCC GATTCTTTCC ATCTGCAGCA GTTTTTCCGT 
GATTCTGATG AGCTCAAGAG TTGGGTCAAT GAGAAGATGA AAACTGCCAC AGATGAAGCT 
TATAAAGATC CATCCAACCT ACAAGGAAAA GTACAGAAGC ATCAGGCTTT TGAGGCTGAG 
CTCTCAGCAA ACCAGAGCCG AATTGATGCC TTGGAGAAAG CTGGCCAAAA GCTGATTGAT 
GTCAACCACT ATGCCAAGGA TGAAGTGGCA GCTCGTATGA ATGAGGTGAT CAGTTTGTGG 
AAGAAACTGC TAGAGGCCAC TGAACTGAAA GGAATAAAGC TTCGTGAAGC CAACCAGCAA 
CAGCAATTTA ATCGCAATGT TGAGGATATT GAATTGTGGC TATATGAAGT AGAAGGTCAC 
TTGGCTTCGG ATGATTACGG CAAAGATCTT ACCAATGTGC AGAACCTCCA GAAGAAACAT 
GCACTGCTAG AGGCAGATGT GGCTGCTCAC CAGGACCGAA TTGATGGCAT CACCATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTT GATGCAGAAA ACATCAAGAA GAAACAGGAA 
GCCCTCGTGG CTCGCTATGA GGCACTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCCGATTCTC TGCGGTTGCA GCAGCTCTTC CGGGATGTTG AGGATGAGGA GACGTGGATT 
CGAGAGAAAG AGCCCATTGC CGCATCTACC AACAGAGGTA AGGATTTAAT TGGGGTCCAG 
AATCTGCTAA AGAAACATCA AGCCTTACAA GCAGAAATTG CTGGACATGA ACCACGCATC 
AAAGCAGTTA CACAGAAGGG GAATGCCATG GTGGAGGAAG GCCATTTTGC TGCAGAGGAT 
GTGAAGGCCA AGCTTCACGA GCTGAACCAA AAGTGGGAGG CACTGAAAGC CAAAGCTTCC 
CAGCGTCGGC AGGACCTGGA GGACTCTCTG CAGGCCCAGC AGTACTTTGC TGATGCTAAC 
GAGGCTGAAT CCTGGATGCG GGAGAAGGAA CCCATTGTGG GCAGCACTGA CTATGGCAAG 
GACGAAGACT CTGCTGAGGC TCTACTGAAG AAACACGAAG CTTTGATGTC AGATCTCAGT 
GCCTACGGCA GCAGCATCCA GGCTTTGCGA GAACAAGCAC AGTCCTGCCG GCAACAAGTG 
GCCCCCACGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CTCTCTACGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAG GGAGATATCC TTACCTTACT CAACAGCACC 
AACAAGGATT GGTGGAAAGT GGAAGTGAAC GATCGTCAGG GTTTTGTGCC GGCTGCGTAC 
GTGAAGAAAT TGGACCCCGC CCAGTCAGCC TCCCGGGAGA ATCTCCTGGA GGAGCAAGGC 
AGCATAGCAC TGCGGCAGGA GCAGATTGAC AATCAATATC ATTCTCTGCT GGAACTGGGT 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TTATGTTGTT CCGTGAAGCG 
AATGAACTAC AGCAATGGAT CAATGAGAAG GAAGCCGCTC TGACAAGTGA GGAGGTCGGA 
GCAGACTTGG AGCAGGTTGA GGTGCTCCAG AAGAAGTTTG ATGACTTCCA GAAGGACCTG 
AAGGCCAATG AGTCACGGTT GAAGGACATT AACAAGGTAG CTGAAGACCT GGAGTCTGAA 
GGTCTCATGG CAGAGGAGGT GCAGGCTGTG CAACAACAGG AAGTGTATGG CATGATGCCC 
AGGGATGAAA CTGATTCCAA GACAGCCTCC CCGTGGAAGT CTGCTCGTCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTACAGCAG 
CTGGCCGAGG AACGGAGCCA GCTCTTGGGC AGCGCCCATG AAGTACAGAG GTTCCACAGA 
GATGCTGATG AAACCAAAGA ATGGATTGAA GAGAAGAATC AAGCTCTAAA CACAGACAAT 
TATGGACATG ATCTCGCCAG TGTCCAGGCC CTGCAACGCA AGCATGAGGG CTTCGAGAGG 
GACCTTGCGG CTCTCGGTGA CAAGGTAAAC TCCCTTGGTG AAACAGCAGA GCGCCTGATC 
CAGTCCCATC CCGAGTCAGC AGAAGACCTG CAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGAGCAGCC TGGGGAAACG TGCAGATCAG CGCAAGGCAA AGTTGGGTGA CTCCCACGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGGGGG 
TTGGTGTCCT CAGATGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCATT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATCGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGACAGC AGCTGTTGGC TCACGGACAC TATGCCAGCC CTGAGATCAA GCAGAAACTT 
GATATTCTTG ACCAGGAGCG TGCAGACCTG GAGAAGGCCT GGGTTCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAACT GCAGCTGTTC CATCGGGACT GTGAGCAAGC TGAGAACTGG 
ATGGCTGCCC GGGAGGCCTT CTTGAATACC GAAGACAAAG GAGACTCACT GGACAGCGTA 
GAGGCTCTGA TCAAAAAACA TGAAGACTTT GACAAAGCGA TTAACGTCCA GGAAGAGAAG 
ATTGCTGCTC TGCAGGCCTT TGCCGACCAG CTCATCGCTG CCGGCCATTA TGCCAAGGGA 
GACATTTCTA GCCGGCGCAA TGAGGTCTTG GACAGGTGGC GACGTCTGAA AGCCCAGATG 
ATTGAGAAAA GGTCAAAGCT AGGAGAATCT CAAACCCTCC AACAGTTCAG CCGGGATGTG 
GATGAGATTG AGGCTTGGAT CAGTGAAAAA TTGCAAACAG CGAGTGATGA GTCGTACAAG 
GATCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ACCAGAAGCA CCAGGCTTTT 
GAAGCAGAGC TGCATGCCAA CGCTGACCGG ATCCGTGGGG TTATCGACAT GGGCAACTCC 
CTCATTGAAC GTGGAGCCTG TGCCGGCAGT GAGGATGCTG TCAAGGCCCG CCTGGCTGCC 
TTAGCTGACC AGTGGCAGTT CTTGGTGCAA AAGTCAGCGG AAAAGAGCCA GAAACTGAAA 
GAAGCCAACA AGCAGCAGAA CTTCAACACA GGGATCAAGG ACTTTGACTT CTGGCTGTCT 
GAGGTGGAGG CCCTGCTGGC ATCCGAAGAT TATGGCAAAG ACCTAGCTTC TGTGAACAAC 
CTGCTGAAAA AGCATCAACT GCTGGAAGCA GATATATCTG CCCATGAGGA TCGCCTGAAG 
GACCTGAACA GCCAGGCAGA CAGCCTGATG ACCAGCAGTG CCTTCGACAC CTCCCAAGTA 
AAGGACAAGA GGGACACCAT CAACGGGCGC TTCCAGAAGA TCAAGAGCAT GGCGGCCTCC 
CGGCGAGCCA AGCTGAATGA ATCCCATCGC CTGCACCAGT TCTTCCGGGA CATGGATGAC 
GAGGAGTCCT GGATCAAGGA GAAGAAGCTG CTGGTGGGCT CAGAGGACTA CGGCCGGGAC 
CTAACCGGCG TGCAGAACCT GAGGAAGAAG CACAAGCGGC TGGAAGCAGA ACTGGCTGCG 
CATGAGCCGG CTATTCAGGG TGTCCTGGAC ACTGGCAAGA AGCTGTCCGA TGACAACACC 
ATCGGGAAAG AGGAGATCCA GCAGCGGCTG GCGCAGTTTG TGGAGCACTG GAAAGAGCTG 
AAGCAGCTGG CAGCTGCCCG GGGTCAGCGG CTGGAAGAGT CCTTGGAATA TCAGCAGTTT 
GTAGCCAATG TGGAAGAGGA AGAAGCCTGG ATCAATGAGA AAATGACCCT GGTGGCCAGC 
GAAGATTATG GCGACACTCT TGCCGCCATC CAGGGCTTAC TGAAGAAACA TGAAGCTTTT 
GAGACAGACT TCACCGTCCA CAAGGATCGC GTGAATGATG TCTGCACCAA TGGACAAGAC 
CTCATTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CAAAGATGAA GGGCCTGAAC 
GGGAAAGTGT CAGACCTGGA GAAAGCTGCA GCCCAGAGAA AGGCGAAGCT GGATGAGAAC 
TCGGCCTTCC TTCAGTTCAA CTGGAAGGCG GACGTGGTGG AGTCCTGGAT CGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGC CGAGACCTGT CTTCTGTGCA GACGCTCCTC 
ACCAAACAGG AAACTTTTGA CGCTGGGCTG CAGGCCTTCC AGCAGGAAGG CATTGCCAAC 
ATCACTGCCC TCAAAGATCA GCTTCTCGCC GCCAAACACG TTCAGTCCAA GGCCATCGAG 
GCCCGGCACG CCTCCCTCAT GAAGAGGTGG AGCCAGCTTC TGGCCAACTC AGCCGCCCGC 
AAGAAGAAGC TTCTGGAGGC TCAGAGTCAC TTCCGCAAGG TGGAGGACCT CTTCCTGACC 
TTCGCCAAAA AGGCTTCTGC CTTCAACAGC TGGTTTGAAA ATGCAGAGGA GGACTTAACA 
GACCCCGTGC GCTGCAACTC CTTGGAAGAA ATCAAAGCTT TGCGCGAGGC CCACGACGCC 
TTCCGCTCCT CCCTCAGCTC TGCCCAGGCT GACTTCAACC AGCTGGCCGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT AGCCTCCAAC CCCTACACCT GGTTTACCAT GGAGGCCCTG 
GAGGAGACCT GGAGGAACCT ACAGAAAATC ATCAAGGAGA GGGAGCTGGA GCTGCAGAAG 
GAACAGCGGC GGCAGGAGGA GAACGACAAG CTGCGCCAGG AGTTTGCCCA GCACGCCAAC 
GCCTTCCACC AGTGGATCCA AGAGACCAGG ACATACCTCC TCGATGGGTC CTGTATGGTG 
GAAGAGTCGG GGACCCTCGA ATCCCAGCTT GAAGCTACCA AACGCAAGCA CCAGGAAATC 
CGAGCCATGA GAAGTCAGCT CAAAAAGATC GAGGACCTGG GGGCCGCCAT GGAGGAGGCC 
CTCATCCTGG ACAACAAGTA CACGGAGCAC AGCACCGTGG GCCTCGCCCA GCAGTGGGAC 
CAGCTGGACC AGCTGGGCAT GCGCATGCAG CACAACCTGG AGCAGCAGAT CCAGGCCAGG 
AACACAACAG GTGTGACTGA GGAGGCCCTC AAAGAATTCA GCATGATGTT TAAACACTTT 
GACAAGGACA AGTCTGGCAG GCTGAACCAT CAGGAGTTCA AATCTTGCCT GCGCTCCCTG 
GGCTATGACC TGCCCATGGT GGAGGAAGGG GAACCTGACC CTGAGTTCGA GGCAATCCTG 
GACACGGTGG ATCCGAACAG AGATGGCCAT GTCTCCTTGC AAGAATACAT GGCTTTCATG 
ATCAGCCGCG AAACTGAGAA CGTCAAGTCC AGCGAGGAGA TTGAGAGCGC CTTCCGGGCC 
CTCAGCTCAG AGGGAAAGCC TTACGTGACC AAGGAGGAGC TCTACCAGAA CCTGACCCGG 
GAACAAGCCG ACTACTGCGT CTCCCACATG AAGCCCTACG TGGACGGCAA GGGCCGCGAG 
CTCCCCACCG CGTTCGACTA CGTGGAGTTC ACCCGCTCGC TTTTCGTGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001362243.1
CDS	729..8102
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELASDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMRRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPTDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGMMPRDETDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKQKLDILDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAAGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINGRFQKIKSMAASRRAKLNESHRLHQFFRDMDDEESWIKEKKLLVGSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHASLMKRWSQLLANSAARKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	NM_001375314.2
Organism	Homo sapiens(human)
Definition	Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant 10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001375314.2

ORF Insert Sequence:

ATGGACCCAA GTGGGGTCAA AGTGCTGGAA ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTAG ACCGATACCA CCGCTTCAAG GAACTCTCAA CCCTTAGGCG TCAGAAGCTG 
GAAGATTCCT ATCGATTCCA GTTCTTTCAA AGAGATGCTG AAGAGCTGGA GAAATGGATA 
CAGGAAAAAC TTCAGATTGC ATCTGATGAG AATTATAAAG ACCCAACCAA CTTGCAGGGA 
AAGCTTCAGA AGCATCAAGC ATTTGAAGCT GAAGTGCAGG CCAACTCAGG AGCCATTGTT 
AAGCTGGATG AAACTGGAAA CCTGATGATC TCAGAAGGGC ATTTTGCATC TGAAACCATA 
CGGACCCGTT TGATGGAGCT GCACCGCCAG TGGGAATTAC TTTTGGAGAA GATGCGAGAA 
AAAGGAATCA AACTGCTGCA GGCCCAGAAG TTGGTGCAGT ACTTACGAGA ATGTGAGGAC 
GTGATGGACT GGATCAATGA CAAGGAAGCA ATTGTTACTT CTGAAGAGCT GGGCCAGGAT 
CTGGAGCATG TAGAGGTTTT ACAGAAGAAA TTTGAAGAGT TTCAAACAGA TATGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTGAACCAG TTTGCTGCCA AACTCATACA GGAGCAGCAC 
CCTGAGGAGG AACTGATCAA GACTAAGCAG GATGAAGTCA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CTCTGCAGAG GCAGGGGAAG CTCTTTGGGG CAGCAGAAGT TCAGCGCTTT 
AACAGGGATG TGGATGAGAC TATCAGTTGG ATTAAGGAAA AGGAGCAGTT AATGGCCTCT 
GATGATTTTG GCCGAGACCT GGCAAGTGTT CAGGCTCTGC TTCGGAAGCA CGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAAGACAAG GTCAAAGCCC TGTGTGCTGA GGCTGACCGC 
CTGCAACAGT CCCACCCTCT GAGTGCAACA CAGATTCAAG TGAAGCGAGA GGAACTGATT 
ACAAACTGGG AGCAGATCCG CACCTTGGCG GCAGAGAGAC ATGCACGGCT CAATGATTCA 
TACAGGCTTC AACGCTTCCT TGCTGACTTC CGTGACCTCA CCAGCTGGGT GACTGAGATG 
AAAGCCCTCA TCAATGCAGA TGAGCTTGCC AGTGATGTGG CTGGGGCTGA AGCCCTGCTA 
GATAGACACC AAGAGCACAA GGGTGAAATT GATGCCCATG AAGACAGCTT CAAATCTGCA 
GATGAATCTG GACAGGCACT GCTTGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGACCG TCCTTTCCGA GGAGAGAGCG GCGCTGCTGG AGCTGTGGGA GCTGCGCAGG 
CAGCAGTACG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GGGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAATGAAG ACTTGGGAGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTTAA GAAGCACGAA GACTTTGAGA AATCCCTTAG TGCCCAGGAG 
GAAAAGATTA CAGCATTAGA TGAATTTGCA ACCAAGCTAA TTCAGAACAA CCACTATGCA 
ATGGAAGATG TGGCCACTCG CCGAGATGCT CTGTTGAGCC GCCGCAATGC CCTTCACGAG 
AGAGCCATGC GTCGCCGGGC CCAGCTAGCC GATTCTTTCC ATCTGCAGCA GTTTTTCCGT 
GATTCTGATG AGCTCAAGAG TTGGGTCAAT GAGAAGATGA AAACTGCCAC AGATGAAGCT 
TATAAAGATC CATCCAACCT ACAAGGAAAA GTACAGAAGC ATCAGGCTTT TGAGGCTGAG 
CTCTCAGCAA ACCAGAGCCG AATTGATGCC TTGGAGAAAG CTGGCCAAAA GCTGATTGAT 
GTCAACCACT ATGCCAAGGA TGAAGTGGCA GCTCGTATGA ATGAGGTGAT CAGTTTGTGG 
AAGAAACTGC TAGAGGCCAC TGAACTGAAA GGAATAAAGC TTCGTGAAGC CAACCAGCAA 
CAGCAATTTA ATCGCAATGT TGAGGATATT GAATTGTGGC TATATGAAGT AGAAGGTCAC 
TTGGCTTCGG ATGATTACGG CAAAGATCTT ACCAATGTGC AGAACCTCCA GAAGAAACAT 
GCACTGCTAG AGGCAGATGT GGCTGCTCAC CAGGACCGAA TTGATGGCAT CACCATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTT GATGCAGAAA ACATCAAGAA GAAACAGGAA 
GCCCTCGTGG CTCGCTATGA GGCACTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCCGATTCTC TGCGGTTGCA GCAGCTCTTC CGGGATGTTG AGGATGAGGA GACGTGGATT 
CGAGAGAAAG AGCCCATTGC CGCATCTACC AACAGAGGTA AGGATTTAAT TGGGGTCCAG 
AATCTGCTAA AGAAACATCA AGCCTTACAA GCAGAAATTG CTGGACATGA ACCACGCATC 
AAAGCAGTTA CACAGAAGGG GAATGCCATG GTGGAGGAAG GCCATTTTGC TGCAGAGGAT 
GTGAAGGCCA AGCTTCACGA GCTGAACCAA AAGTGGGAGG CACTGAAAGC CAAAGCTTCC 
CAGCGTCGGC AGGACCTGGA GGACTCTCTG CAGGCCCAGC AGTACTTTGC TGATGCTAAC 
GAGGCTGAAT CCTGGATGCG GGAGAAGGAA CCCATTGTGG GCAGCACTGA CTATGGCAAG 
GACGAAGACT CTGCTGAGGC TCTACTGAAG AAACACGAAG CTTTGATGTC AGATCTCAGT 
GCCTACGGCA GCAGCATCCA GGCTTTGCGA GAACAAGCAC AGTCCTGCCG GCAACAAGTG 
GCCCCCACGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CTCTCTACGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAG GGAGATATCC TTACCTTACT CAACAGCACC 
AACAAGGATT GGTGGAAAGT GGAAGTGAAC GATCGTCAGG GTTTTGTGCC GGCTGCGTAC 
GTGAAGAAAT TGGACCCCGC CCAGTCAGCC TCCCGGGAGA ATCTCCTGGA GGAGCAAGGC 
AGCATAGCAC TGCGGCAGGA GCAGATTGAC AATCAATATC ATTCTCTGCT GGAACTGGGT 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TTATGTTGTT CCGTGAAGCG 
AATGAACTAC AGCAATGGAT CAATGAGAAG GAAGCCGCTC TGACAAGTGA GGAGGTCGGA 
GCAGACTTGG AGCAGGTTGA GGTGCTCCAG AAGAAGTTTG ATGACTTCCA GAAGGACCTG 
AAGGCCAATG AGTCACGGTT GAAGGACATT AACAAGGTAG CTGAAGACCT GGAGTCTGAA 
GGTCTCATGG CAGAGGAGGT GCAGGCTGTG CAACAACAGG AAGTGTATGG CATGATGCCC 
AGGGATGAAA CTGATTCCAA GACAGCCTCC CCGTGGAAGT CTGCTCGTCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTACAGCAG 
CTGGCCGAGG AACGGAGCCA GCTCTTGGGC AGCGCCCATG AAGTACAGAG GTTCCACAGA 
GATGCTGATG AAACCAAAGA ATGGATTGAA GAGAAGAATC AAGCTCTAAA CACAGACAAT 
TATGGACATG ATCTCGCCAG TGTCCAGGCC CTGCAACGCA AGCATGAGGG CTTCGAGAGG 
GACCTTGCGG CTCTCGGTGA CAAGGTAAAC TCCCTTGGTG AAACAGCAGA GCGCCTGATC 
CAGTCCCATC CCGAGTCAGC AGAAGACCTG CAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGAGCAGCC TGGGGAAACG TGCAGATCAG CGCAAGGCAA AGTTGGGTGA CTCCCACGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGGGGG 
TTGGTGTCCT CAGATGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCATT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATCGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGACAGC AGCTGTTGGC TCACGGACAC TATGCCAGCC CTGAGATCAA GCAGAAACTT 
GATATTCTTG ACCAGGAGCG TGCAGACCTG GAGAAGGCCT GGGTTCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAACT GCAGCTGTTC CATCGGGACT GTGAGCAAGC TGAGAACTGG 
ATGGCTGCCC GGGAGGCCTT CTTGAATACC GAAGACAAAG GAGACTCACT GGACAGCGTA 
GAGGCTCTGA TCAAAAAACA TGAAGACTTT GACAAAGCGA TTAACGTCCA GGAAGAGAAG 
ATTGCTGCTC TGCAGGCCTT TGCCGACCAG CTCATCGCTG CCGGCCATTA TGCCAAGGGA 
GACATTTCTA GCCGGCGCAA TGAGGTCTTG GACAGGTGGC GACGTCTGAA AGCCCAGATG 
ATTGAGAAAA GGTCAAAGCT AGGAGAATCT CAAACCCTCC AACAGTTCAG CCGGGATGTG 
GATGAGATTG AGGCTTGGAT CAGTGAAAAA TTGCAAACAG CGAGTGATGA GTCGTACAAG 
GATCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ACCAGAAGCA CCAGGCTTTT 
GAAGCAGAGC TGCATGCCAA CGCTGACCGG ATCCGTGGGG TTATCGACAT GGGCAACTCC 
CTCATTGAAC GTGGAGCCTG TGCCGGCAGT GAGGATGCTG TCAAGGCCCG CCTGGCTGCC 
TTAGCTGACC AGTGGCAGTT CTTGGTGCAA AAGTCAGCGG AAAAGAGCCA GAAACTGAAA 
GAAGCCAACA AGCAGCAGAA CTTCAACACA GGGATCAAGG ACTTTGACTT CTGGCTGTCT 
GAGGTGGAGG CCCTGCTGGC ATCCGAAGAT TATGGCAAAG ACCTAGCTTC TGTGAACAAC 
CTGCTGAAAA AGCATCAACT GCTGGAAGCA GATATATCTG CCCATGAGGA TCGCCTGAAG 
GACCTGAACA GCCAGGCAGA CAGCCTGATG ACCAGCAGTG CCTTCGACAC CTCCCAAGTA 
AAGGACAAGA GGGACACCAT CAACGGGCGC TTCCAGAAGA TCAAGAGCAT GGCGGCCTCC 
CGGCGAGCCA AGCTGAATGA ATCCCATCGC CTGCACCAGT TCTTCCGGGA CATGGATGAC 
GAGGAGTCCT GGATCAAGGA GAAGAAGCTG CTGGTGGGCT CAGAGGACTA CGGCCGGGAC 
CTAACCGGCG TGCAGAACCT GAGGAAGAAG CACAAGCGGC TGGAAGCAGA ACTGGCTGCG 
CATGAGCCGG CTATTCAGGG TGTCCTGGAC ACTGGCAAGA AGCTGTCCGA TGACAACACC 
ATCGGGAAAG AGGAGATCCA GCAGCGGCTG GCGCAGTTTG TGGAGCACTG GAAAGAGCTG 
AAGCAGCTGG CAGCTGCCCG GGGTCAGCGG CTGGAAGAGT CCTTGGAATA TCAGCAGTTT 
GTAGCCAATG TGGAAGAGGA AGAAGCCTGG ATCAATGAGA AAATGACCCT GGTGGCCAGC 
GAAGATTATG GCGACACTCT TGCCGCCATC CAGGGCTTAC TGAAGAAACA TGAAGCTTTT 
GAGACAGACT TCACCGTCCA CAAGGATCGC GTGAATGATG TCTGCACCAA TGGACAAGAC 
CTCATTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CAAAGATGAA GGGCCTGAAC 
GGGAAAGTGT CAGACCTGGA GAAAGCTGCA GCCCAGAGAA AGGCGAAGCT GGATGAGAAC 
TCGGCCTTCC TTCAGTTCAA CTGGAAGGCG GACGTGGTGG AGTCCTGGAT CGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGC CGAGACCTGT CTTCTGTGCA GACGCTCCTC 
ACCAAACAGG AAACTTTTGA CGCTGGGCTG CAGGCCTTCC AGCAGGAAGG CATTGCCAAC 
ATCACTGCCC TCAAAGATCA GCTTCTCGCC GCCAAACACG TTCAGTCCAA GGCCATCGAG 
GCCCGGCACG CCTCCCTCAT GAAGAGGTGG AGCCAGCTTC TGGCCAACTC AGCCGCCCGC 
AAGAAGAAGC TTCTGGAGGC TCAGAGTCAC TTCCGCAAGG TGGAGGACCT CTTCCTGACC 
TTCGCCAAAA AGGCTTCTGC CTTCAACAGC TGGTTTGAAA ATGCAGAGGA GGACTTAACA 
GACCCCGTGC GCTGCAACTC CTTGGAAGAA ATCAAAGCTT TGCGCGAGGC CCACGACGCC 
TTCCGCTCCT CCCTCAGCTC TGCCCAGGCT GACTTCAACC AGCTGGCCGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT AGCCTCCAAC CCCTACACCT GGTTTACCAT GGAGGCCCTG 
GAGGAGACCT GGAGGAACCT ACAGAAAATC ATCAAGGAGA GGGAGCTGGA GCTGCAGAAG 
GAACAGCGGC GGCAGGAGGA GAACGACAAG CTGCGCCAGG AGTTTGCCCA GCACGCCAAC 
GCCTTCCACC AGTGGATCCA AGAGACCAGG ACATACCTCC TCGATGGGTC CTGTATGGTG 
GAAGAGTCGG GGACCCTCGA ATCCCAGCTT GAAGCTACCA AACGCAAGCA CCAGGAAATC 
CGAGCCATGA GAAGTCAGCT CAAAAAGATC GAGGACCTGG GGGCCGCCAT GGAGGAGGCC 
CTCATCCTGG ACAACAAGTA CACGGAGCAC AGCACCGTGG GCCTCGCCCA GCAGTGGGAC 
CAGCTGGACC AGCTGGGCAT GCGCATGCAG CACAACCTGG AGCAGCAGAT CCAGGCCAGG 
AACACAACAG GTGTGACTGA GGAGGCCCTC AAAGAATTCA GCATGATGTT TAAACACTTT 
GACAAGGACA AGTCTGGCAG GCTGAACCAT CAGGAGTTCA AATCTTGCCT GCGCTCCCTG 
GGCTATGACC TGCCCATGGT GGAGGAAGGG GAACCTGACC CTGAGTTCGA GGCAATCCTG 
GACACGGTGG ATCCGAACAG AGATGGCCAT GTCTCCTTGC AAGAATACAT GGCTTTCATG 
ATCAGCCGCG AAACTGAGAA CGTCAAGTCC AGCGAGGAGA TTGAGAGCGC CTTCCGGGCC 
CTCAGCTCAG AGGGAAAGCC TTACGTGACC AAGGAGGAGC TCTACCAGAA CCTGACCCGG 
GAACAAGCCG ACTACTGCGT CTCCCACATG AAGCCCTACG TGGACGGCAA GGGCCGCGAG 
CTCCCCACCG CGTTCGACTA CGTGGAGTTC ACCCGCTCGC TTTTCGTGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( NM_001363765.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001350694.1 Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant 5, mRNA.

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CloneID	OHu98857
Clone ID Related Accession (Same CDS sequence)	NM_001363765.2 , NM_001375314.2 , XM_017015061.1 , NM_001363765.1
Accession Version	NM_001363765.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Homo sapiens(human)
Product	spectrin alpha chain, non-erythrocytic 1 isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL356481.16. On May 29, 2018 this sequence version replaced XM_017015061.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.249773.1, SRR1803612.276815.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGGACCCAA GTGGGGTCAA AGTGCTGGAA ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTAG ACCGATACCA CCGCTTCAAG GAACTCTCAA CCCTTAGGCG TCAGAAGCTG 
GAAGATTCCT ATCGATTCCA GTTCTTTCAA AGAGATGCTG AAGAGCTGGA GAAATGGATA 
CAGGAAAAAC TTCAGATTGC ATCTGATGAG AATTATAAAG ACCCAACCAA CTTGCAGGGA 
AAGCTTCAGA AGCATCAAGC ATTTGAAGCT GAAGTGCAGG CCAACTCAGG AGCCATTGTT 
AAGCTGGATG AAACTGGAAA CCTGATGATC TCAGAAGGGC ATTTTGCATC TGAAACCATA 
CGGACCCGTT TGATGGAGCT GCACCGCCAG TGGGAATTAC TTTTGGAGAA GATGCGAGAA 
AAAGGAATCA AACTGCTGCA GGCCCAGAAG TTGGTGCAGT ACTTACGAGA ATGTGAGGAC 
GTGATGGACT GGATCAATGA CAAGGAAGCA ATTGTTACTT CTGAAGAGCT GGGCCAGGAT 
CTGGAGCATG TAGAGGTTTT ACAGAAGAAA TTTGAAGAGT TTCAAACAGA TATGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTGAACCAG TTTGCTGCCA AACTCATACA GGAGCAGCAC 
CCTGAGGAGG AACTGATCAA GACTAAGCAG GATGAAGTCA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CTCTGCAGAG GCAGGGGAAG CTCTTTGGGG CAGCAGAAGT TCAGCGCTTT 
AACAGGGATG TGGATGAGAC TATCAGTTGG ATTAAGGAAA AGGAGCAGTT AATGGCCTCT 
GATGATTTTG GCCGAGACCT GGCAAGTGTT CAGGCTCTGC TTCGGAAGCA CGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAAGACAAG GTCAAAGCCC TGTGTGCTGA GGCTGACCGC 
CTGCAACAGT CCCACCCTCT GAGTGCAACA CAGATTCAAG TGAAGCGAGA GGAACTGATT 
ACAAACTGGG AGCAGATCCG CACCTTGGCG GCAGAGAGAC ATGCACGGCT CAATGATTCA 
TACAGGCTTC AACGCTTCCT TGCTGACTTC CGTGACCTCA CCAGCTGGGT GACTGAGATG 
AAAGCCCTCA TCAATGCAGA TGAGCTTGCC AGTGATGTGG CTGGGGCTGA AGCCCTGCTA 
GATAGACACC AAGAGCACAA GGGTGAAATT GATGCCCATG AAGACAGCTT CAAATCTGCA 
GATGAATCTG GACAGGCACT GCTTGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGACCG TCCTTTCCGA GGAGAGAGCG GCGCTGCTGG AGCTGTGGGA GCTGCGCAGG 
CAGCAGTACG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GGGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAATGAAG ACTTGGGAGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTTAA GAAGCACGAA GACTTTGAGA AATCCCTTAG TGCCCAGGAG 
GAAAAGATTA CAGCATTAGA TGAATTTGCA ACCAAGCTAA TTCAGAACAA CCACTATGCA 
ATGGAAGATG TGGCCACTCG CCGAGATGCT CTGTTGAGCC GCCGCAATGC CCTTCACGAG 
AGAGCCATGC GTCGCCGGGC CCAGCTAGCC GATTCTTTCC ATCTGCAGCA GTTTTTCCGT 
GATTCTGATG AGCTCAAGAG TTGGGTCAAT GAGAAGATGA AAACTGCCAC AGATGAAGCT 
TATAAAGATC CATCCAACCT ACAAGGAAAA GTACAGAAGC ATCAGGCTTT TGAGGCTGAG 
CTCTCAGCAA ACCAGAGCCG AATTGATGCC TTGGAGAAAG CTGGCCAAAA GCTGATTGAT 
GTCAACCACT ATGCCAAGGA TGAAGTGGCA GCTCGTATGA ATGAGGTGAT CAGTTTGTGG 
AAGAAACTGC TAGAGGCCAC TGAACTGAAA GGAATAAAGC TTCGTGAAGC CAACCAGCAA 
CAGCAATTTA ATCGCAATGT TGAGGATATT GAATTGTGGC TATATGAAGT AGAAGGTCAC 
TTGGCTTCGG ATGATTACGG CAAAGATCTT ACCAATGTGC AGAACCTCCA GAAGAAACAT 
GCACTGCTAG AGGCAGATGT GGCTGCTCAC CAGGACCGAA TTGATGGCAT CACCATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTT GATGCAGAAA ACATCAAGAA GAAACAGGAA 
GCCCTCGTGG CTCGCTATGA GGCACTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCCGATTCTC TGCGGTTGCA GCAGCTCTTC CGGGATGTTG AGGATGAGGA GACGTGGATT 
CGAGAGAAAG AGCCCATTGC CGCATCTACC AACAGAGGTA AGGATTTAAT TGGGGTCCAG 
AATCTGCTAA AGAAACATCA AGCCTTACAA GCAGAAATTG CTGGACATGA ACCACGCATC 
AAAGCAGTTA CACAGAAGGG GAATGCCATG GTGGAGGAAG GCCATTTTGC TGCAGAGGAT 
GTGAAGGCCA AGCTTCACGA GCTGAACCAA AAGTGGGAGG CACTGAAAGC CAAAGCTTCC 
CAGCGTCGGC AGGACCTGGA GGACTCTCTG CAGGCCCAGC AGTACTTTGC TGATGCTAAC 
GAGGCTGAAT CCTGGATGCG GGAGAAGGAA CCCATTGTGG GCAGCACTGA CTATGGCAAG 
GACGAAGACT CTGCTGAGGC TCTACTGAAG AAACACGAAG CTTTGATGTC AGATCTCAGT 
GCCTACGGCA GCAGCATCCA GGCTTTGCGA GAACAAGCAC AGTCCTGCCG GCAACAAGTG 
GCCCCCACGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CTCTCTACGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAG GGAGATATCC TTACCTTACT CAACAGCACC 
AACAAGGATT GGTGGAAAGT GGAAGTGAAC GATCGTCAGG GTTTTGTGCC GGCTGCGTAC 
GTGAAGAAAT TGGACCCCGC CCAGTCAGCC TCCCGGGAGA ATCTCCTGGA GGAGCAAGGC 
AGCATAGCAC TGCGGCAGGA GCAGATTGAC AATCAATATC ATTCTCTGCT GGAACTGGGT 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TTATGTTGTT CCGTGAAGCG 
AATGAACTAC AGCAATGGAT CAATGAGAAG GAAGCCGCTC TGACAAGTGA GGAGGTCGGA 
GCAGACTTGG AGCAGGTTGA GGTGCTCCAG AAGAAGTTTG ATGACTTCCA GAAGGACCTG 
AAGGCCAATG AGTCACGGTT GAAGGACATT AACAAGGTAG CTGAAGACCT GGAGTCTGAA 
GGTCTCATGG CAGAGGAGGT GCAGGCTGTG CAACAACAGG AAGTGTATGG CATGATGCCC 
AGGGATGAAA CTGATTCCAA GACAGCCTCC CCGTGGAAGT CTGCTCGTCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTACAGCAG 
CTGGCCGAGG AACGGAGCCA GCTCTTGGGC AGCGCCCATG AAGTACAGAG GTTCCACAGA 
GATGCTGATG AAACCAAAGA ATGGATTGAA GAGAAGAATC AAGCTCTAAA CACAGACAAT 
TATGGACATG ATCTCGCCAG TGTCCAGGCC CTGCAACGCA AGCATGAGGG CTTCGAGAGG 
GACCTTGCGG CTCTCGGTGA CAAGGTAAAC TCCCTTGGTG AAACAGCAGA GCGCCTGATC 
CAGTCCCATC CCGAGTCAGC AGAAGACCTG CAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGAGCAGCC TGGGGAAACG TGCAGATCAG CGCAAGGCAA AGTTGGGTGA CTCCCACGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGGGGG 
TTGGTGTCCT CAGATGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCATT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATCGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGACAGC AGCTGTTGGC TCACGGACAC TATGCCAGCC CTGAGATCAA GCAGAAACTT 
GATATTCTTG ACCAGGAGCG TGCAGACCTG GAGAAGGCCT GGGTTCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAACT GCAGCTGTTC CATCGGGACT GTGAGCAAGC TGAGAACTGG 
ATGGCTGCCC GGGAGGCCTT CTTGAATACC GAAGACAAAG GAGACTCACT GGACAGCGTA 
GAGGCTCTGA TCAAAAAACA TGAAGACTTT GACAAAGCGA TTAACGTCCA GGAAGAGAAG 
ATTGCTGCTC TGCAGGCCTT TGCCGACCAG CTCATCGCTG CCGGCCATTA TGCCAAGGGA 
GACATTTCTA GCCGGCGCAA TGAGGTCTTG GACAGGTGGC GACGTCTGAA AGCCCAGATG 
ATTGAGAAAA GGTCAAAGCT AGGAGAATCT CAAACCCTCC AACAGTTCAG CCGGGATGTG 
GATGAGATTG AGGCTTGGAT CAGTGAAAAA TTGCAAACAG CGAGTGATGA GTCGTACAAG 
GATCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ACCAGAAGCA CCAGGCTTTT 
GAAGCAGAGC TGCATGCCAA CGCTGACCGG ATCCGTGGGG TTATCGACAT GGGCAACTCC 
CTCATTGAAC GTGGAGCCTG TGCCGGCAGT GAGGATGCTG TCAAGGCCCG CCTGGCTGCC 
TTAGCTGACC AGTGGCAGTT CTTGGTGCAA AAGTCAGCGG AAAAGAGCCA GAAACTGAAA 
GAAGCCAACA AGCAGCAGAA CTTCAACACA GGGATCAAGG ACTTTGACTT CTGGCTGTCT 
GAGGTGGAGG CCCTGCTGGC ATCCGAAGAT TATGGCAAAG ACCTAGCTTC TGTGAACAAC 
CTGCTGAAAA AGCATCAACT GCTGGAAGCA GATATATCTG CCCATGAGGA TCGCCTGAAG 
GACCTGAACA GCCAGGCAGA CAGCCTGATG ACCAGCAGTG CCTTCGACAC CTCCCAAGTA 
AAGGACAAGA GGGACACCAT CAACGGGCGC TTCCAGAAGA TCAAGAGCAT GGCGGCCTCC 
CGGCGAGCCA AGCTGAATGA ATCCCATCGC CTGCACCAGT TCTTCCGGGA CATGGATGAC 
GAGGAGTCCT GGATCAAGGA GAAGAAGCTG CTGGTGGGCT CAGAGGACTA CGGCCGGGAC 
CTAACCGGCG TGCAGAACCT GAGGAAGAAG CACAAGCGGC TGGAAGCAGA ACTGGCTGCG 
CATGAGCCGG CTATTCAGGG TGTCCTGGAC ACTGGCAAGA AGCTGTCCGA TGACAACACC 
ATCGGGAAAG AGGAGATCCA GCAGCGGCTG GCGCAGTTTG TGGAGCACTG GAAAGAGCTG 
AAGCAGCTGG CAGCTGCCCG GGGTCAGCGG CTGGAAGAGT CCTTGGAATA TCAGCAGTTT 
GTAGCCAATG TGGAAGAGGA AGAAGCCTGG ATCAATGAGA AAATGACCCT GGTGGCCAGC 
GAAGATTATG GCGACACTCT TGCCGCCATC CAGGGCTTAC TGAAGAAACA TGAAGCTTTT 
GAGACAGACT TCACCGTCCA CAAGGATCGC GTGAATGATG TCTGCACCAA TGGACAAGAC 
CTCATTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CAAAGATGAA GGGCCTGAAC 
GGGAAAGTGT CAGACCTGGA GAAAGCTGCA GCCCAGAGAA AGGCGAAGCT GGATGAGAAC 
TCGGCCTTCC TTCAGTTCAA CTGGAAGGCG GACGTGGTGG AGTCCTGGAT CGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGC CGAGACCTGT CTTCTGTGCA GACGCTCCTC 
ACCAAACAGG AAACTTTTGA CGCTGGGCTG CAGGCCTTCC AGCAGGAAGG CATTGCCAAC 
ATCACTGCCC TCAAAGATCA GCTTCTCGCC GCCAAACACG TTCAGTCCAA GGCCATCGAG 
GCCCGGCACG CCTCCCTCAT GAAGAGGTGG AGCCAGCTTC TGGCCAACTC AGCCGCCCGC 
AAGAAGAAGC TTCTGGAGGC TCAGAGTCAC TTCCGCAAGG TGGAGGACCT CTTCCTGACC 
TTCGCCAAAA AGGCTTCTGC CTTCAACAGC TGGTTTGAAA ATGCAGAGGA GGACTTAACA 
GACCCCGTGC GCTGCAACTC CTTGGAAGAA ATCAAAGCTT TGCGCGAGGC CCACGACGCC 
TTCCGCTCCT CCCTCAGCTC TGCCCAGGCT GACTTCAACC AGCTGGCCGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT AGCCTCCAAC CCCTACACCT GGTTTACCAT GGAGGCCCTG 
GAGGAGACCT GGAGGAACCT ACAGAAAATC ATCAAGGAGA GGGAGCTGGA GCTGCAGAAG 
GAACAGCGGC GGCAGGAGGA GAACGACAAG CTGCGCCAGG AGTTTGCCCA GCACGCCAAC 
GCCTTCCACC AGTGGATCCA AGAGACCAGG ACATACCTCC TCGATGGGTC CTGTATGGTG 
GAAGAGTCGG GGACCCTCGA ATCCCAGCTT GAAGCTACCA AACGCAAGCA CCAGGAAATC 
CGAGCCATGA GAAGTCAGCT CAAAAAGATC GAGGACCTGG GGGCCGCCAT GGAGGAGGCC 
CTCATCCTGG ACAACAAGTA CACGGAGCAC AGCACCGTGG GCCTCGCCCA GCAGTGGGAC 
CAGCTGGACC AGCTGGGCAT GCGCATGCAG CACAACCTGG AGCAGCAGAT CCAGGCCAGG 
AACACAACAG GTGTGACTGA GGAGGCCCTC AAAGAATTCA GCATGATGTT TAAACACTTT 
GACAAGGACA AGTCTGGCAG GCTGAACCAT CAGGAGTTCA AATCTTGCCT GCGCTCCCTG 
GGCTATGACC TGCCCATGGT GGAGGAAGGG GAACCTGACC CTGAGTTCGA GGCAATCCTG 
GACACGGTGG ATCCGAACAG AGATGGCCAT GTCTCCTTGC AAGAATACAT GGCTTTCATG 
ATCAGCCGCG AAACTGAGAA CGTCAAGTCC AGCGAGGAGA TTGAGAGCGC CTTCCGGGCC 
CTCAGCTCAG AGGGAAAGCC TTACGTGACC AAGGAGGAGC TCTACCAGAA CCTGACCCGG 
GAACAAGCCG ACTACTGCGT CTCCCACATG AAGCCCTACG TGGACGGCAA GGGCCGCGAG 
CTCCCCACCG CGTTCGACTA CGTGGAGTTC ACCCGCTCGC TTTTCGTGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350694.1
CDS	114..7487
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELASDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMRRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPTDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGMMPRDETDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKQKLDILDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAAGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINGRFQKIKSMAASRRAKLNESHRLHQFFRDMDDEESWIKEKKLLVGSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHASLMKRWSQLLANSAARKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	NM_001363765.1
Organism	Homo sapiens(human)
Definition	Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363765.1

ORF Insert Sequence:

ATGGACCCAA GTGGGGTCAA AGTGCTGGAA ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTAG ACCGATACCA CCGCTTCAAG GAACTCTCAA CCCTTAGGCG TCAGAAGCTG 
GAAGATTCCT ATCGATTCCA GTTCTTTCAA AGAGATGCTG AAGAGCTGGA GAAATGGATA 
CAGGAAAAAC TTCAGATTGC ATCTGATGAG AATTATAAAG ACCCAACCAA CTTGCAGGGA 
AAGCTTCAGA AGCATCAAGC ATTTGAAGCT GAAGTGCAGG CCAACTCAGG AGCCATTGTT 
AAGCTGGATG AAACTGGAAA CCTGATGATC TCAGAAGGGC ATTTTGCATC TGAAACCATA 
CGGACCCGTT TGATGGAGCT GCACCGCCAG TGGGAATTAC TTTTGGAGAA GATGCGAGAA 
AAAGGAATCA AACTGCTGCA GGCCCAGAAG TTGGTGCAGT ACTTACGAGA ATGTGAGGAC 
GTGATGGACT GGATCAATGA CAAGGAAGCA ATTGTTACTT CTGAAGAGCT GGGCCAGGAT 
CTGGAGCATG TAGAGGTTTT ACAGAAGAAA TTTGAAGAGT TTCAAACAGA TATGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTGAACCAG TTTGCTGCCA AACTCATACA GGAGCAGCAC 
CCTGAGGAGG AACTGATCAA GACTAAGCAG GATGAAGTCA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CTCTGCAGAG GCAGGGGAAG CTCTTTGGGG CAGCAGAAGT TCAGCGCTTT 
AACAGGGATG TGGATGAGAC TATCAGTTGG ATTAAGGAAA AGGAGCAGTT AATGGCCTCT 
GATGATTTTG GCCGAGACCT GGCAAGTGTT CAGGCTCTGC TTCGGAAGCA CGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAAGACAAG GTCAAAGCCC TGTGTGCTGA GGCTGACCGC 
CTGCAACAGT CCCACCCTCT GAGTGCAACA CAGATTCAAG TGAAGCGAGA GGAACTGATT 
ACAAACTGGG AGCAGATCCG CACCTTGGCG GCAGAGAGAC ATGCACGGCT CAATGATTCA 
TACAGGCTTC AACGCTTCCT TGCTGACTTC CGTGACCTCA CCAGCTGGGT GACTGAGATG 
AAAGCCCTCA TCAATGCAGA TGAGCTTGCC AGTGATGTGG CTGGGGCTGA AGCCCTGCTA 
GATAGACACC AAGAGCACAA GGGTGAAATT GATGCCCATG AAGACAGCTT CAAATCTGCA 
GATGAATCTG GACAGGCACT GCTTGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGACCG TCCTTTCCGA GGAGAGAGCG GCGCTGCTGG AGCTGTGGGA GCTGCGCAGG 
CAGCAGTACG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GGGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAATGAAG ACTTGGGAGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTTAA GAAGCACGAA GACTTTGAGA AATCCCTTAG TGCCCAGGAG 
GAAAAGATTA CAGCATTAGA TGAATTTGCA ACCAAGCTAA TTCAGAACAA CCACTATGCA 
ATGGAAGATG TGGCCACTCG CCGAGATGCT CTGTTGAGCC GCCGCAATGC CCTTCACGAG 
AGAGCCATGC GTCGCCGGGC CCAGCTAGCC GATTCTTTCC ATCTGCAGCA GTTTTTCCGT 
GATTCTGATG AGCTCAAGAG TTGGGTCAAT GAGAAGATGA AAACTGCCAC AGATGAAGCT 
TATAAAGATC CATCCAACCT ACAAGGAAAA GTACAGAAGC ATCAGGCTTT TGAGGCTGAG 
CTCTCAGCAA ACCAGAGCCG AATTGATGCC TTGGAGAAAG CTGGCCAAAA GCTGATTGAT 
GTCAACCACT ATGCCAAGGA TGAAGTGGCA GCTCGTATGA ATGAGGTGAT CAGTTTGTGG 
AAGAAACTGC TAGAGGCCAC TGAACTGAAA GGAATAAAGC TTCGTGAAGC CAACCAGCAA 
CAGCAATTTA ATCGCAATGT TGAGGATATT GAATTGTGGC TATATGAAGT AGAAGGTCAC 
TTGGCTTCGG ATGATTACGG CAAAGATCTT ACCAATGTGC AGAACCTCCA GAAGAAACAT 
GCACTGCTAG AGGCAGATGT GGCTGCTCAC CAGGACCGAA TTGATGGCAT CACCATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTT GATGCAGAAA ACATCAAGAA GAAACAGGAA 
GCCCTCGTGG CTCGCTATGA GGCACTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCCGATTCTC TGCGGTTGCA GCAGCTCTTC CGGGATGTTG AGGATGAGGA GACGTGGATT 
CGAGAGAAAG AGCCCATTGC CGCATCTACC AACAGAGGTA AGGATTTAAT TGGGGTCCAG 
AATCTGCTAA AGAAACATCA AGCCTTACAA GCAGAAATTG CTGGACATGA ACCACGCATC 
AAAGCAGTTA CACAGAAGGG GAATGCCATG GTGGAGGAAG GCCATTTTGC TGCAGAGGAT 
GTGAAGGCCA AGCTTCACGA GCTGAACCAA AAGTGGGAGG CACTGAAAGC CAAAGCTTCC 
CAGCGTCGGC AGGACCTGGA GGACTCTCTG CAGGCCCAGC AGTACTTTGC TGATGCTAAC 
GAGGCTGAAT CCTGGATGCG GGAGAAGGAA CCCATTGTGG GCAGCACTGA CTATGGCAAG 
GACGAAGACT CTGCTGAGGC TCTACTGAAG AAACACGAAG CTTTGATGTC AGATCTCAGT 
GCCTACGGCA GCAGCATCCA GGCTTTGCGA GAACAAGCAC AGTCCTGCCG GCAACAAGTG 
GCCCCCACGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CTCTCTACGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAG GGAGATATCC TTACCTTACT CAACAGCACC 
AACAAGGATT GGTGGAAAGT GGAAGTGAAC GATCGTCAGG GTTTTGTGCC GGCTGCGTAC 
GTGAAGAAAT TGGACCCCGC CCAGTCAGCC TCCCGGGAGA ATCTCCTGGA GGAGCAAGGC 
AGCATAGCAC TGCGGCAGGA GCAGATTGAC AATCAATATC ATTCTCTGCT GGAACTGGGT 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TTATGTTGTT CCGTGAAGCG 
AATGAACTAC AGCAATGGAT CAATGAGAAG GAAGCCGCTC TGACAAGTGA GGAGGTCGGA 
GCAGACTTGG AGCAGGTTGA GGTGCTCCAG AAGAAGTTTG ATGACTTCCA GAAGGACCTG 
AAGGCCAATG AGTCACGGTT GAAGGACATT AACAAGGTAG CTGAAGACCT GGAGTCTGAA 
GGTCTCATGG CAGAGGAGGT GCAGGCTGTG CAACAACAGG AAGTGTATGG CATGATGCCC 
AGGGATGAAA CTGATTCCAA GACAGCCTCC CCGTGGAAGT CTGCTCGTCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTACAGCAG 
CTGGCCGAGG AACGGAGCCA GCTCTTGGGC AGCGCCCATG AAGTACAGAG GTTCCACAGA 
GATGCTGATG AAACCAAAGA ATGGATTGAA GAGAAGAATC AAGCTCTAAA CACAGACAAT 
TATGGACATG ATCTCGCCAG TGTCCAGGCC CTGCAACGCA AGCATGAGGG CTTCGAGAGG 
GACCTTGCGG CTCTCGGTGA CAAGGTAAAC TCCCTTGGTG AAACAGCAGA GCGCCTGATC 
CAGTCCCATC CCGAGTCAGC AGAAGACCTG CAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGAGCAGCC TGGGGAAACG TGCAGATCAG CGCAAGGCAA AGTTGGGTGA CTCCCACGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGGGGG 
TTGGTGTCCT CAGATGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCATT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATCGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGACAGC AGCTGTTGGC TCACGGACAC TATGCCAGCC CTGAGATCAA GCAGAAACTT 
GATATTCTTG ACCAGGAGCG TGCAGACCTG GAGAAGGCCT GGGTTCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAACT GCAGCTGTTC CATCGGGACT GTGAGCAAGC TGAGAACTGG 
ATGGCTGCCC GGGAGGCCTT CTTGAATACC GAAGACAAAG GAGACTCACT GGACAGCGTA 
GAGGCTCTGA TCAAAAAACA TGAAGACTTT GACAAAGCGA TTAACGTCCA GGAAGAGAAG 
ATTGCTGCTC TGCAGGCCTT TGCCGACCAG CTCATCGCTG CCGGCCATTA TGCCAAGGGA 
GACATTTCTA GCCGGCGCAA TGAGGTCTTG GACAGGTGGC GACGTCTGAA AGCCCAGATG 
ATTGAGAAAA GGTCAAAGCT AGGAGAATCT CAAACCCTCC AACAGTTCAG CCGGGATGTG 
GATGAGATTG AGGCTTGGAT CAGTGAAAAA TTGCAAACAG CGAGTGATGA GTCGTACAAG 
GATCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ACCAGAAGCA CCAGGCTTTT 
GAAGCAGAGC TGCATGCCAA CGCTGACCGG ATCCGTGGGG TTATCGACAT GGGCAACTCC 
CTCATTGAAC GTGGAGCCTG TGCCGGCAGT GAGGATGCTG TCAAGGCCCG CCTGGCTGCC 
TTAGCTGACC AGTGGCAGTT CTTGGTGCAA AAGTCAGCGG AAAAGAGCCA GAAACTGAAA 
GAAGCCAACA AGCAGCAGAA CTTCAACACA GGGATCAAGG ACTTTGACTT CTGGCTGTCT 
GAGGTGGAGG CCCTGCTGGC ATCCGAAGAT TATGGCAAAG ACCTAGCTTC TGTGAACAAC 
CTGCTGAAAA AGCATCAACT GCTGGAAGCA GATATATCTG CCCATGAGGA TCGCCTGAAG 
GACCTGAACA GCCAGGCAGA CAGCCTGATG ACCAGCAGTG CCTTCGACAC CTCCCAAGTA 
AAGGACAAGA GGGACACCAT CAACGGGCGC TTCCAGAAGA TCAAGAGCAT GGCGGCCTCC 
CGGCGAGCCA AGCTGAATGA ATCCCATCGC CTGCACCAGT TCTTCCGGGA CATGGATGAC 
GAGGAGTCCT GGATCAAGGA GAAGAAGCTG CTGGTGGGCT CAGAGGACTA CGGCCGGGAC 
CTAACCGGCG TGCAGAACCT GAGGAAGAAG CACAAGCGGC TGGAAGCAGA ACTGGCTGCG 
CATGAGCCGG CTATTCAGGG TGTCCTGGAC ACTGGCAAGA AGCTGTCCGA TGACAACACC 
ATCGGGAAAG AGGAGATCCA GCAGCGGCTG GCGCAGTTTG TGGAGCACTG GAAAGAGCTG 
AAGCAGCTGG CAGCTGCCCG GGGTCAGCGG CTGGAAGAGT CCTTGGAATA TCAGCAGTTT 
GTAGCCAATG TGGAAGAGGA AGAAGCCTGG ATCAATGAGA AAATGACCCT GGTGGCCAGC 
GAAGATTATG GCGACACTCT TGCCGCCATC CAGGGCTTAC TGAAGAAACA TGAAGCTTTT 
GAGACAGACT TCACCGTCCA CAAGGATCGC GTGAATGATG TCTGCACCAA TGGACAAGAC 
CTCATTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CAAAGATGAA GGGCCTGAAC 
GGGAAAGTGT CAGACCTGGA GAAAGCTGCA GCCCAGAGAA AGGCGAAGCT GGATGAGAAC 
TCGGCCTTCC TTCAGTTCAA CTGGAAGGCG GACGTGGTGG AGTCCTGGAT CGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGC CGAGACCTGT CTTCTGTGCA GACGCTCCTC 
ACCAAACAGG AAACTTTTGA CGCTGGGCTG CAGGCCTTCC AGCAGGAAGG CATTGCCAAC 
ATCACTGCCC TCAAAGATCA GCTTCTCGCC GCCAAACACG TTCAGTCCAA GGCCATCGAG 
GCCCGGCACG CCTCCCTCAT GAAGAGGTGG AGCCAGCTTC TGGCCAACTC AGCCGCCCGC 
AAGAAGAAGC TTCTGGAGGC TCAGAGTCAC TTCCGCAAGG TGGAGGACCT CTTCCTGACC 
TTCGCCAAAA AGGCTTCTGC CTTCAACAGC TGGTTTGAAA ATGCAGAGGA GGACTTAACA 
GACCCCGTGC GCTGCAACTC CTTGGAAGAA ATCAAAGCTT TGCGCGAGGC CCACGACGCC 
TTCCGCTCCT CCCTCAGCTC TGCCCAGGCT GACTTCAACC AGCTGGCCGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT AGCCTCCAAC CCCTACACCT GGTTTACCAT GGAGGCCCTG 
GAGGAGACCT GGAGGAACCT ACAGAAAATC ATCAAGGAGA GGGAGCTGGA GCTGCAGAAG 
GAACAGCGGC GGCAGGAGGA GAACGACAAG CTGCGCCAGG AGTTTGCCCA GCACGCCAAC 
GCCTTCCACC AGTGGATCCA AGAGACCAGG ACATACCTCC TCGATGGGTC CTGTATGGTG 
GAAGAGTCGG GGACCCTCGA ATCCCAGCTT GAAGCTACCA AACGCAAGCA CCAGGAAATC 
CGAGCCATGA GAAGTCAGCT CAAAAAGATC GAGGACCTGG GGGCCGCCAT GGAGGAGGCC 
CTCATCCTGG ACAACAAGTA CACGGAGCAC AGCACCGTGG GCCTCGCCCA GCAGTGGGAC 
CAGCTGGACC AGCTGGGCAT GCGCATGCAG CACAACCTGG AGCAGCAGAT CCAGGCCAGG 
AACACAACAG GTGTGACTGA GGAGGCCCTC AAAGAATTCA GCATGATGTT TAAACACTTT 
GACAAGGACA AGTCTGGCAG GCTGAACCAT CAGGAGTTCA AATCTTGCCT GCGCTCCCTG 
GGCTATGACC TGCCCATGGT GGAGGAAGGG GAACCTGACC CTGAGTTCGA GGCAATCCTG 
GACACGGTGG ATCCGAACAG AGATGGCCAT GTCTCCTTGC AAGAATACAT GGCTTTCATG 
ATCAGCCGCG AAACTGAGAA CGTCAAGTCC AGCGAGGAGA TTGAGAGCGC CTTCCGGGCC 
CTCAGCTCAG AGGGAAAGCC TTACGTGACC AAGGAGGAGC TCTACCAGAA CCTGACCCGG 
GAACAAGCCG ACTACTGCGT CTCCCACATG AAGCCCTACG TGGACGGCAA GGGCCGCGAG 
CTCCCCACCG CGTTCGACTA CGTGGAGTTC ACCCGCTCGC TTTTCGTGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_017015059.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016870548.1 Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X9, mRNA.

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CloneID	OHu98855
Clone ID Related Accession (Same CDS sequence)	NM_001375313.1 , XM_017015059.1
Accession Version	XM_017015059.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7416bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-12
Organism	Homo sapiens(human)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END##

ATGGACCCAA GTGGGGTCAA AGTGCTGGAA ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTAG ACCGATACCA CCGCTTCAAG GAACTCTCAA CCCTTAGGCG TCAGAAGCTG 
GAAGATTCCT ATCGATTCCA GTTCTTTCAA AGAGATGCTG AAGAGCTGGA GAAATGGATA 
CAGGAAAAAC TTCAGATTGC ATCTGATGAG AATTATAAAG ACCCAACCAA CTTGCAGGGA 
AAGCTTCAGA AGCATCAAGC ATTTGAAGCT GAAGTGCAGG CCAACTCAGG AGCCATTGTT 
AAGCTGGATG AAACTGGAAA CCTGATGATC TCAGAAGGGC ATTTTGCATC TGAAACCATA 
CGGACCCGTT TGATGGAGCT GCACCGCCAG TGGGAATTAC TTTTGGAGAA GATGCGAGAA 
AAAGGAATCA AACTGCTGCA GGCCCAGAAG TTGGTGCAGT ACTTACGAGA ATGTGAGGAC 
GTGATGGACT GGATCAATGA CAAGGAAGCA ATTGTTACTT CTGAAGAGCT GGGCCAGGAT 
CTGGAGCATG TAGAGGTTTT ACAGAAGAAA TTTGAAGAGT TTCAAACAGA TATGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTGAACCAG TTTGCTGCCA AACTCATACA GGAGCAGCAC 
CCTGAGGAGG AACTGATCAA GACTAAGCAG GATGAAGTCA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CTCTGCAGAG GCAGGGGAAG CTCTTTGGGG CAGCAGAAGT TCAGCGCTTT 
AACAGGGATG TGGATGAGAC TATCAGTTGG ATTAAGGAAA AGGAGCAGTT AATGGCCTCT 
GATGATTTTG GCCGAGACCT GGCAAGTGTT CAGGCTCTGC TTCGGAAGCA CGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAAGACAAG GTCAAAGCCC TGTGTGCTGA GGCTGACCGC 
CTGCAACAGT CCCACCCTCT GAGTGCAACA CAGATTCAAG TGAAGCGAGA GGAACTGATT 
ACAAACTGGG AGCAGATCCG CACCTTGGCG GCAGAGAGAC ATGCACGGCT CAATGATTCA 
TACAGGCTTC AACGCTTCCT TGCTGACTTC CGTGACCTCA CCAGCTGGGT GACTGAGATG 
AAAGCCCTCA TCAATGCAGA TGAGCTTGCC AGTGATGTGG CTGGGGCTGA AGCCCTGCTA 
GATAGACACC AAGAGCACAA GGGTGAAATT GATGCCCATG AAGACAGCTT CAAATCTGCA 
GATGAATCTG GACAGGCACT GCTTGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGACCG TCCTTTCCGA GGAGAGAGCG GCGCTGCTGG AGCTGTGGGA GCTGCGCAGG 
CAGCAGTACG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GGGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAATGAAG ACTTGGGAGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTTAA GAAGCACGAA GACTTTGAGA AATCCCTTAG TGCCCAGGAG 
GAAAAGATTA CAGCATTAGA TGAATTTGCA ACCAAGCTAA TTCAGAACAA CCACTATGCA 
ATGGAAGATG TGGCCACTCG CCGAGATGCT CTGTTGAGCC GCCGCAATGC CCTTCACGAG 
AGAGCCATGC GTCGCCGGGC CCAGCTAGCC GATTCTTTCC ATCTGCAGCA GTTTTTCCGT 
GATTCTGATG AGCTCAAGAG TTGGGTCAAT GAGAAGATGA AAACTGCCAC AGATGAAGCT 
TATAAAGATC CATCCAACCT ACAAGGAAAA GTACAGAAGC ATCAGGCTTT TGAGGCTGAG 
CTCTCAGCAA ACCAGAGCCG AATTGATGCC TTGGAGAAAG CTGGCCAAAA GCTGATTGAT 
GTCAACCACT ATGCCAAGGA TGAAGTGGCA GCTCGTATGA ATGAGGTGAT CAGTTTGTGG 
AAGAAACTGC TAGAGGCCAC TGAACTGAAA GGAATAAAGC TTCGTGAAGC CAACCAGCAA 
CAGCAATTTA ATCGCAATGT TGAGGATATT GAATTGTGGC TATATGAAGT AGAAGGTCAC 
TTGGCTTCGG ATGATTACGG CAAAGATCTT ACCAATGTGC AGAACCTCCA GAAGAAACAT 
GCACTGCTAG AGGCAGATGT GGCTGCTCAC CAGGACCGAA TTGATGGCAT CACCATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTT GATGCAGAAA ACATCAAGAA GAAACAGGAA 
GCCCTCGTGG CTCGCTATGA GGCACTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCCGATTCTC TGCGGTTGCA GCAGCTCTTC CGGGATGTTG AGGATGAGGA GACGTGGATT 
CGAGAGAAAG AGCCCATTGC CGCATCTACC AACAGAGGTA AGGATTTAAT TGGGGTCCAG 
AATCTGCTAA AGAAACATCA AGCCTTACAA GCAGAAATTG CTGGACATGA ACCACGCATC 
AAAGCAGTTA CACAGAAGGG GAATGCCATG GTGGAGGAAG GCCATTTTGC TGCAGAGGAT 
GTGAAGGCCA AGCTTCACGA GCTGAACCAA AAGTGGGAGG CACTGAAAGC CAAAGCTTCC 
CAGCGTCGGC AGGACCTGGA GGACTCTCTG CAGGCCCAGC AGTACTTTGC TGATGCTAAC 
GAGGCTGAAT CCTGGATGCG GGAGAAGGAA CCCATTGTGG GCAGCACTGA CTATGGCAAG 
GACGAAGACT CTGCTGAGGC TCTACTGAAG AAACACGAAG CTTTGATGTC AGATCTCAGT 
GCCTACGGCA GCAGCATCCA GGCTTTGCGA GAACAAGCAC AGTCCTGCCG GCAACAAGTG 
GCCCCCACGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CTCTCTACGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAG GGAGATATCC TTACCTTACT CAACAGCACC 
AACAAGGATT GGTGGAAAGT GGAAGTGAAC GATCGTCAGG GTTTTGTGCC GGCTGCGTAC 
GTGAAGAAAT TGGACCCCGC CCAGTCAGCC TCCCGGGAGA ATCTCCTGGA GGAGCAAGGC 
AGCATAGCAC TGCGGCAGGA GCAGATTGAC AATCAGACAC GCATAACTAA GGAGGCCGGC 
AGTGTATCTC TGCGTATGAA GCAGGTGGAA GAACTATATC ATTCTCTGCT GGAACTGGGT 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TTATGTTGTT CCGTGAAGCG 
AATGAACTAC AGCAATGGAT CAATGAGAAG GAAGCCGCTC TGACAAGTGA GGAGGTCGGA 
GCAGACTTGG AGCAGGTTGA GGTGCTCCAG AAGAAGTTTG ATGACTTCCA GAAGGACCTG 
AAGGCCAATG AGTCACGGTT GAAGGACATT AACAAGGTAG CTGAAGACCT GGAGTCTGAA 
GGTCTCATGG CAGAGGAGGT GCAGGCTGTG CAACAACAGG AAGTGTATGG CATGATGCCC 
AGGGATGAAA CTGATTCCAA GACAGCCTCC CCGTGGAAGT CTGCTCGTCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTACAGCAG 
CTGGCCGAGG AACGGAGCCA GCTCTTGGGC AGCGCCCATG AAGTACAGAG GTTCCACAGA 
GATGCTGATG AAACCAAAGA ATGGATTGAA GAGAAGAATC AAGCTCTAAA CACAGACAAT 
TATGGACATG ATCTCGCCAG TGTCCAGGCC CTGCAACGCA AGCATGAGGG CTTCGAGAGG 
GACCTTGCGG CTCTCGGTGA CAAGGTAAAC TCCCTTGGTG AAACAGCAGA GCGCCTGATC 
CAGTCCCATC CCGAGTCAGC AGAAGACCTG CAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGAGCAGCC TGGGGAAACG TGCAGATCAG CGCAAGGCAA AGTTGGGTGA CTCCCACGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGGGGG 
TTGGTGTCCT CAGATGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCATT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATCGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGACAGC AGCTGTTGGC TCACGGACAC TATGCCAGCC CTGAGATCAA GCAGAAACTT 
GATATTCTTG ACCAGGAGCG TGCAGACCTG GAGAAGGCCT GGGTTCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAACT GCAGCTGTTC CATCGGGACT GTGAGCAAGC TGAGAACTGG 
ATGGCTGCCC GGGAGGCCTT CTTGAATACC GAAGACAAAG GAGACTCACT GGACAGCGTA 
GAGGCTCTGA TCAAAAAACA TGAAGACTTT GACAAAGCGA TTAACGTCCA GGAAGAGAAG 
ATTGCTGCTC TGCAGGCCTT TGCCGACCAG CTCATCGCTG CCGGCCATTA TGCCAAGGGA 
GACATTTCTA GCCGGCGCAA TGAGGTCTTG GACAGGTGGC GACGTCTGAA AGCCCAGATG 
ATTGAGAAAA GGTCAAAGCT AGGAGAATCT CAAACCCTCC AACAGTTCAG CCGGGATGTG 
GATGAGATTG AGGCTTGGAT CAGTGAAAAA TTGCAAACAG CGAGTGATGA GTCGTACAAG 
GATCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ACCAGAAGCA CCAGGCTTTT 
GAAGCAGAGC TGCATGCCAA CGCTGACCGG ATCCGTGGGG TTATCGACAT GGGCAACTCC 
CTCATTGAAC GTGGAGCCTG TGCCGGCAGT GAGGATGCTG TCAAGGCCCG CCTGGCTGCC 
TTAGCTGACC AGTGGCAGTT CTTGGTGCAA AAGTCAGCGG AAAAGAGCCA GAAACTGAAA 
GAAGCCAACA AGCAGCAGAA CTTCAACACA GGGATCAAGG ACTTTGACTT CTGGCTGTCT 
GAGGTGGAGG CCCTGCTGGC ATCCGAAGAT TATGGCAAAG ACCTAGCTTC TGTGAACAAC 
CTGCTGAAAA AGCATCAACT GCTGGAAGCA GATATATCTG CCCATGAGGA TCGCCTGAAG 
GACCTGAACA GCCAGGCAGA CAGCCTGATG ACCAGCAGTG CCTTCGACAC CTCCCAAGTA 
AAGGACAAGA GGGACACCAT CAACGGGCGC TTCCAGAAGA TCAAGAGCAT GGCGGCCTCC 
CGGCGAGCCA AGCTGAATGA ATCCCATCGC CTGCACCAGT TCTTCCGGGA CATGGATGAC 
GAGGAGTCCT GGATCAAGGA GAAGAAGCTG CTGGTGGGCT CAGAGGACTA CGGCCGGGAC 
CTAACCGGCG TGCAGAACCT GAGGAAGAAG CACAAGCGGC TGGAAGCAGA ACTGGCTGCG 
CATGAGCCGG CTATTCAGGG TGTCCTGGAC ACTGGCAAGA AGCTGTCCGA TGACAACACC 
ATCGGGAAAG AGGAGATCCA GCAGCGGCTG GCGCAGTTTG TGGAGCACTG GAAAGAGCTG 
AAGCAGCTGG CAGCTGCCCG GGGTCAGCGG CTGGAAGAGT CCTTGGAATA TCAGCAGTTT 
GTAGCCAATG TGGAAGAGGA AGAAGCCTGG ATCAATGAGA AAATGACCCT GGTGGCCAGC 
GAAGATTATG GCGACACTCT TGCCGCCATC CAGGGCTTAC TGAAGAAACA TGAAGCTTTT 
GAGACAGACT TCACCGTCCA CAAGGATCGC GTGAATGATG TCTGCACCAA TGGACAAGAC 
CTCATTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CAAAGATGAA GGGCCTGAAC 
GGGAAAGTGT CAGACCTGGA GAAAGCTGCA GCCCAGAGAA AGGCGAAGCT GGATGAGAAC 
TCGGCCTTCC TTCAGTTCAA CTGGAAGGCG GACGTGGTGG AGTCCTGGAT CGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGC CGAGACCTGT CTTCTGTGCA GACGCTCCTC 
ACCAAACAGG AAACTTTTGA CGCTGGGCTG CAGGCCTTCC AGCAGGAAGG CATTGCCAAC 
ATCACTGCCC TCAAAGATCA GCTTCTCGCC GCCAAACACG TTCAGTCCAA GGCCATCGAG 
GCCCGGCACG CCTCCCTCAT GAAGAGGTGG AGCCAGCTTC TGGCCAACTC AGCCGCCCGC 
AAGAAGAAGC TTCTGGAGGC TCAGAGTCAC TTCCGCAAGG TGGAGGACCT CTTCCTGACC 
TTCGCCAAAA AGGCTTCTGC CTTCAACAGC TGGTTTGAAA ATGCAGAGGA GGACTTAACA 
GACCCCGTGC GCTGCAACTC CTTGGAAGAA ATCAAAGCTT TGCGCGAGGC CCACGACGCC 
TTCCGCTCCT CCCTCAGCTC TGCCCAGGCT GACTTCAACC AGCTGGCCGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT AGCCTCCAAC CCCTACACCT GGTTTACCAT GGAGGCCCTG 
GAGGAGACCT GGAGGAACCT ACAGAAAATC ATCAAGGAGA GGGAGCTGGA GCTGCAGAAG 
GAACAGCGGC GGCAGGAGGA GAACGACAAG CTGCGCCAGG AGTTTGCCCA GCACGCCAAC 
GCCTTCCACC AGTGGATCCA AGAGACCAGG TCCTGTATGG TGGAAGAGTC GGGGACCCTC 
GAATCCCAGC TTGAAGCTAC CAAACGCAAG CACCAGGAAA TCCGAGCCAT GAGAAGTCAG 
CTCAAAAAGA TCGAGGACCT GGGGGCCGCC ATGGAGGAGG CCCTCATCCT GGACAACAAG 
TACACGGAGC ACAGCACCGT GGGCCTCGCC CAGCAGTGGG ACCAGCTGGA CCAGCTGGGC 
ATGCGCATGC AGCACAACCT GGAGCAGCAG ATCCAGGCCA GGAACACAAC AGGTGTGACT 
GAGGAGGCCC TCAAAGAATT CAGCATGATG TTTAAACACT TTGACAAGGA CAAGTCTGGC 
AGGCTGAACC ATCAGGAGTT CAAATCTTGC CTGCGCTCCC TGGGCTATGA CCTGCCCATG 
GTGGAGGAAG GGGAACCTGA CCCTGAGTTC GAGGCAATCC TGGACACGGT GGATCCGAAC 
AGAGATGGCC ATGTCTCCTT GCAAGAATAC ATGGCTTTCA TGATCAGCCG CGAAACTGAG 
AACGTCAAGT CCAGCGAGGA GATTGAGAGC GCCTTCCGGG CCCTCAGCTC AGAGGGAAAG 
CCTTACGTGA CCAAGGAGGA GCTCTACCAG AACCTGACCC GGGAACAAGC CGACTACTGC 
GTCTCCCACA TGAAGCCCTA CGTGGACGGC AAGGGCCGCG AGCTCCCCAC CGCGTTCGAC 
TACGTGGAGT TCACCCGCTC GCTTTTCGTG AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016870548.1
CDS	128..7543
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELASDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMRRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPTDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRITKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGMMPRDETDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKQKLDILDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAAGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINGRFQKIKSMAASRRAKLNESHRLHQFFRDMDDEESWIKEKKLLVGSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHASLMKRWSQLLANSAARKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_017015059.1
Organism	Homo sapiens(human)
Definition	Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017015059.1

ORF Insert Sequence:

ATGGACCCAA GTGGGGTCAA AGTGCTGGAA ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTAG ACCGATACCA CCGCTTCAAG GAACTCTCAA CCCTTAGGCG TCAGAAGCTG 
GAAGATTCCT ATCGATTCCA GTTCTTTCAA AGAGATGCTG AAGAGCTGGA GAAATGGATA 
CAGGAAAAAC TTCAGATTGC ATCTGATGAG AATTATAAAG ACCCAACCAA CTTGCAGGGA 
AAGCTTCAGA AGCATCAAGC ATTTGAAGCT GAAGTGCAGG CCAACTCAGG AGCCATTGTT 
AAGCTGGATG AAACTGGAAA CCTGATGATC TCAGAAGGGC ATTTTGCATC TGAAACCATA 
CGGACCCGTT TGATGGAGCT GCACCGCCAG TGGGAATTAC TTTTGGAGAA GATGCGAGAA 
AAAGGAATCA AACTGCTGCA GGCCCAGAAG TTGGTGCAGT ACTTACGAGA ATGTGAGGAC 
GTGATGGACT GGATCAATGA CAAGGAAGCA ATTGTTACTT CTGAAGAGCT GGGCCAGGAT 
CTGGAGCATG TAGAGGTTTT ACAGAAGAAA TTTGAAGAGT TTCAAACAGA TATGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTGAACCAG TTTGCTGCCA AACTCATACA GGAGCAGCAC 
CCTGAGGAGG AACTGATCAA GACTAAGCAG GATGAAGTCA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CTCTGCAGAG GCAGGGGAAG CTCTTTGGGG CAGCAGAAGT TCAGCGCTTT 
AACAGGGATG TGGATGAGAC TATCAGTTGG ATTAAGGAAA AGGAGCAGTT AATGGCCTCT 
GATGATTTTG GCCGAGACCT GGCAAGTGTT CAGGCTCTGC TTCGGAAGCA CGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAAGACAAG GTCAAAGCCC TGTGTGCTGA GGCTGACCGC 
CTGCAACAGT CCCACCCTCT GAGTGCAACA CAGATTCAAG TGAAGCGAGA GGAACTGATT 
ACAAACTGGG AGCAGATCCG CACCTTGGCG GCAGAGAGAC ATGCACGGCT CAATGATTCA 
TACAGGCTTC AACGCTTCCT TGCTGACTTC CGTGACCTCA CCAGCTGGGT GACTGAGATG 
AAAGCCCTCA TCAATGCAGA TGAGCTTGCC AGTGATGTGG CTGGGGCTGA AGCCCTGCTA 
GATAGACACC AAGAGCACAA GGGTGAAATT GATGCCCATG AAGACAGCTT CAAATCTGCA 
GATGAATCTG GACAGGCACT GCTTGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGACCG TCCTTTCCGA GGAGAGAGCG GCGCTGCTGG AGCTGTGGGA GCTGCGCAGG 
CAGCAGTACG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GGGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAATGAAG ACTTGGGAGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTTAA GAAGCACGAA GACTTTGAGA AATCCCTTAG TGCCCAGGAG 
GAAAAGATTA CAGCATTAGA TGAATTTGCA ACCAAGCTAA TTCAGAACAA CCACTATGCA 
ATGGAAGATG TGGCCACTCG CCGAGATGCT CTGTTGAGCC GCCGCAATGC CCTTCACGAG 
AGAGCCATGC GTCGCCGGGC CCAGCTAGCC GATTCTTTCC ATCTGCAGCA GTTTTTCCGT 
GATTCTGATG AGCTCAAGAG TTGGGTCAAT GAGAAGATGA AAACTGCCAC AGATGAAGCT 
TATAAAGATC CATCCAACCT ACAAGGAAAA GTACAGAAGC ATCAGGCTTT TGAGGCTGAG 
CTCTCAGCAA ACCAGAGCCG AATTGATGCC TTGGAGAAAG CTGGCCAAAA GCTGATTGAT 
GTCAACCACT ATGCCAAGGA TGAAGTGGCA GCTCGTATGA ATGAGGTGAT CAGTTTGTGG 
AAGAAACTGC TAGAGGCCAC TGAACTGAAA GGAATAAAGC TTCGTGAAGC CAACCAGCAA 
CAGCAATTTA ATCGCAATGT TGAGGATATT GAATTGTGGC TATATGAAGT AGAAGGTCAC 
TTGGCTTCGG ATGATTACGG CAAAGATCTT ACCAATGTGC AGAACCTCCA GAAGAAACAT 
GCACTGCTAG AGGCAGATGT GGCTGCTCAC CAGGACCGAA TTGATGGCAT CACCATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTT GATGCAGAAA ACATCAAGAA GAAACAGGAA 
GCCCTCGTGG CTCGCTATGA GGCACTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCCGATTCTC TGCGGTTGCA GCAGCTCTTC CGGGATGTTG AGGATGAGGA GACGTGGATT 
CGAGAGAAAG AGCCCATTGC CGCATCTACC AACAGAGGTA AGGATTTAAT TGGGGTCCAG 
AATCTGCTAA AGAAACATCA AGCCTTACAA GCAGAAATTG CTGGACATGA ACCACGCATC 
AAAGCAGTTA CACAGAAGGG GAATGCCATG GTGGAGGAAG GCCATTTTGC TGCAGAGGAT 
GTGAAGGCCA AGCTTCACGA GCTGAACCAA AAGTGGGAGG CACTGAAAGC CAAAGCTTCC 
CAGCGTCGGC AGGACCTGGA GGACTCTCTG CAGGCCCAGC AGTACTTTGC TGATGCTAAC 
GAGGCTGAAT CCTGGATGCG GGAGAAGGAA CCCATTGTGG GCAGCACTGA CTATGGCAAG 
GACGAAGACT CTGCTGAGGC TCTACTGAAG AAACACGAAG CTTTGATGTC AGATCTCAGT 
GCCTACGGCA GCAGCATCCA GGCTTTGCGA GAACAAGCAC AGTCCTGCCG GCAACAAGTG 
GCCCCCACGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CTCTCTACGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAG GGAGATATCC TTACCTTACT CAACAGCACC 
AACAAGGATT GGTGGAAAGT GGAAGTGAAC GATCGTCAGG GTTTTGTGCC GGCTGCGTAC 
GTGAAGAAAT TGGACCCCGC CCAGTCAGCC TCCCGGGAGA ATCTCCTGGA GGAGCAAGGC 
AGCATAGCAC TGCGGCAGGA GCAGATTGAC AATCAGACAC GCATAACTAA GGAGGCCGGC 
AGTGTATCTC TGCGTATGAA GCAGGTGGAA GAACTATATC ATTCTCTGCT GGAACTGGGT 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TTATGTTGTT CCGTGAAGCG 
AATGAACTAC AGCAATGGAT CAATGAGAAG GAAGCCGCTC TGACAAGTGA GGAGGTCGGA 
GCAGACTTGG AGCAGGTTGA GGTGCTCCAG AAGAAGTTTG ATGACTTCCA GAAGGACCTG 
AAGGCCAATG AGTCACGGTT GAAGGACATT AACAAGGTAG CTGAAGACCT GGAGTCTGAA 
GGTCTCATGG CAGAGGAGGT GCAGGCTGTG CAACAACAGG AAGTGTATGG CATGATGCCC 
AGGGATGAAA CTGATTCCAA GACAGCCTCC CCGTGGAAGT CTGCTCGTCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTACAGCAG 
CTGGCCGAGG AACGGAGCCA GCTCTTGGGC AGCGCCCATG AAGTACAGAG GTTCCACAGA 
GATGCTGATG AAACCAAAGA ATGGATTGAA GAGAAGAATC AAGCTCTAAA CACAGACAAT 
TATGGACATG ATCTCGCCAG TGTCCAGGCC CTGCAACGCA AGCATGAGGG CTTCGAGAGG 
GACCTTGCGG CTCTCGGTGA CAAGGTAAAC TCCCTTGGTG AAACAGCAGA GCGCCTGATC 
CAGTCCCATC CCGAGTCAGC AGAAGACCTG CAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGAGCAGCC TGGGGAAACG TGCAGATCAG CGCAAGGCAA AGTTGGGTGA CTCCCACGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGGGGG 
TTGGTGTCCT CAGATGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCATT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATCGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGACAGC AGCTGTTGGC TCACGGACAC TATGCCAGCC CTGAGATCAA GCAGAAACTT 
GATATTCTTG ACCAGGAGCG TGCAGACCTG GAGAAGGCCT GGGTTCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAACT GCAGCTGTTC CATCGGGACT GTGAGCAAGC TGAGAACTGG 
ATGGCTGCCC GGGAGGCCTT CTTGAATACC GAAGACAAAG GAGACTCACT GGACAGCGTA 
GAGGCTCTGA TCAAAAAACA TGAAGACTTT GACAAAGCGA TTAACGTCCA GGAAGAGAAG 
ATTGCTGCTC TGCAGGCCTT TGCCGACCAG CTCATCGCTG CCGGCCATTA TGCCAAGGGA 
GACATTTCTA GCCGGCGCAA TGAGGTCTTG GACAGGTGGC GACGTCTGAA AGCCCAGATG 
ATTGAGAAAA GGTCAAAGCT AGGAGAATCT CAAACCCTCC AACAGTTCAG CCGGGATGTG 
GATGAGATTG AGGCTTGGAT CAGTGAAAAA TTGCAAACAG CGAGTGATGA GTCGTACAAG 
GATCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ACCAGAAGCA CCAGGCTTTT 
GAAGCAGAGC TGCATGCCAA CGCTGACCGG ATCCGTGGGG TTATCGACAT GGGCAACTCC 
CTCATTGAAC GTGGAGCCTG TGCCGGCAGT GAGGATGCTG TCAAGGCCCG CCTGGCTGCC 
TTAGCTGACC AGTGGCAGTT CTTGGTGCAA AAGTCAGCGG AAAAGAGCCA GAAACTGAAA 
GAAGCCAACA AGCAGCAGAA CTTCAACACA GGGATCAAGG ACTTTGACTT CTGGCTGTCT 
GAGGTGGAGG CCCTGCTGGC ATCCGAAGAT TATGGCAAAG ACCTAGCTTC TGTGAACAAC 
CTGCTGAAAA AGCATCAACT GCTGGAAGCA GATATATCTG CCCATGAGGA TCGCCTGAAG 
GACCTGAACA GCCAGGCAGA CAGCCTGATG ACCAGCAGTG CCTTCGACAC CTCCCAAGTA 
AAGGACAAGA GGGACACCAT CAACGGGCGC TTCCAGAAGA TCAAGAGCAT GGCGGCCTCC 
CGGCGAGCCA AGCTGAATGA ATCCCATCGC CTGCACCAGT TCTTCCGGGA CATGGATGAC 
GAGGAGTCCT GGATCAAGGA GAAGAAGCTG CTGGTGGGCT CAGAGGACTA CGGCCGGGAC 
CTAACCGGCG TGCAGAACCT GAGGAAGAAG CACAAGCGGC TGGAAGCAGA ACTGGCTGCG 
CATGAGCCGG CTATTCAGGG TGTCCTGGAC ACTGGCAAGA AGCTGTCCGA TGACAACACC 
ATCGGGAAAG AGGAGATCCA GCAGCGGCTG GCGCAGTTTG TGGAGCACTG GAAAGAGCTG 
AAGCAGCTGG CAGCTGCCCG GGGTCAGCGG CTGGAAGAGT CCTTGGAATA TCAGCAGTTT 
GTAGCCAATG TGGAAGAGGA AGAAGCCTGG ATCAATGAGA AAATGACCCT GGTGGCCAGC 
GAAGATTATG GCGACACTCT TGCCGCCATC CAGGGCTTAC TGAAGAAACA TGAAGCTTTT 
GAGACAGACT TCACCGTCCA CAAGGATCGC GTGAATGATG TCTGCACCAA TGGACAAGAC 
CTCATTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CAAAGATGAA GGGCCTGAAC 
GGGAAAGTGT CAGACCTGGA GAAAGCTGCA GCCCAGAGAA AGGCGAAGCT GGATGAGAAC 
TCGGCCTTCC TTCAGTTCAA CTGGAAGGCG GACGTGGTGG AGTCCTGGAT CGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGC CGAGACCTGT CTTCTGTGCA GACGCTCCTC 
ACCAAACAGG AAACTTTTGA CGCTGGGCTG CAGGCCTTCC AGCAGGAAGG CATTGCCAAC 
ATCACTGCCC TCAAAGATCA GCTTCTCGCC GCCAAACACG TTCAGTCCAA GGCCATCGAG 
GCCCGGCACG CCTCCCTCAT GAAGAGGTGG AGCCAGCTTC TGGCCAACTC AGCCGCCCGC 
AAGAAGAAGC TTCTGGAGGC TCAGAGTCAC TTCCGCAAGG TGGAGGACCT CTTCCTGACC 
TTCGCCAAAA AGGCTTCTGC CTTCAACAGC TGGTTTGAAA ATGCAGAGGA GGACTTAACA 
GACCCCGTGC GCTGCAACTC CTTGGAAGAA ATCAAAGCTT TGCGCGAGGC CCACGACGCC 
TTCCGCTCCT CCCTCAGCTC TGCCCAGGCT GACTTCAACC AGCTGGCCGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT AGCCTCCAAC CCCTACACCT GGTTTACCAT GGAGGCCCTG 
GAGGAGACCT GGAGGAACCT ACAGAAAATC ATCAAGGAGA GGGAGCTGGA GCTGCAGAAG 
GAACAGCGGC GGCAGGAGGA GAACGACAAG CTGCGCCAGG AGTTTGCCCA GCACGCCAAC 
GCCTTCCACC AGTGGATCCA AGAGACCAGG TCCTGTATGG TGGAAGAGTC GGGGACCCTC 
GAATCCCAGC TTGAAGCTAC CAAACGCAAG CACCAGGAAA TCCGAGCCAT GAGAAGTCAG 
CTCAAAAAGA TCGAGGACCT GGGGGCCGCC ATGGAGGAGG CCCTCATCCT GGACAACAAG 
TACACGGAGC ACAGCACCGT GGGCCTCGCC CAGCAGTGGG ACCAGCTGGA CCAGCTGGGC 
ATGCGCATGC AGCACAACCT GGAGCAGCAG ATCCAGGCCA GGAACACAAC AGGTGTGACT 
GAGGAGGCCC TCAAAGAATT CAGCATGATG TTTAAACACT TTGACAAGGA CAAGTCTGGC 
AGGCTGAACC ATCAGGAGTT CAAATCTTGC CTGCGCTCCC TGGGCTATGA CCTGCCCATG 
GTGGAGGAAG GGGAACCTGA CCCTGAGTTC GAGGCAATCC TGGACACGGT GGATCCGAAC 
AGAGATGGCC ATGTCTCCTT GCAAGAATAC ATGGCTTTCA TGATCAGCCG CGAAACTGAG 
AACGTCAAGT CCAGCGAGGA GATTGAGAGC GCCTTCCGGG CCCTCAGCTC AGAGGGAAAG 
CCTTACGTGA CCAAGGAGGA GCTCTACCAG AACCTGACCC GGGAACAAGC CGACTACTGC 
GTCTCCCACA TGAAGCCCTA CGTGGACGGC AAGGGCCGCG AGCTCCCCAC CGCGTTCGAC 
TACGTGGAGT TCACCCGCTC GCTTTTCGTG AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_017015060.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016870549.1 Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X12, mRNA.

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CloneID	OHu98856
Clone ID Related Accession (Same CDS sequence)	XM_017015060.1
Accession Version	XM_017015060.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7392bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACCG ATACCACCGC 
TTCAAGGAAC TCTCAACCCT TAGGCGTCAG AAGCTGGAAG ATTCCTATCG ATTCCAGTTC 
TTTCAAAGAG ATGCTGAAGA GCTGGAGAAA TGGATACAGG AAAAACTTCA GATTGCATCT 
GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGAAAGC TTCAGAAGCA TCAAGCATTT 
GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAGC TGGATGAAAC TGGAAACCTG 
ATGATCTCAG AAGGGCATTT TGCATCTGAA ACCATACGGA CCCGTTTGAT GGAGCTGCAC 
CGCCAGTGGG AATTACTTTT GGAGAAGATG CGAGAAAAAG GAATCAAACT GCTGCAGGCC 
CAGAAGTTGG TGCAGTACTT ACGAGAATGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCAATTG TTACTTCTGA AGAGCTGGGC CAGGATCTGG AGCATGTAGA GGTTTTACAG 
AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCTCATG AAGAAAGAGT TAATGAAGTG 
AACCAGTTTG CTGCCAAACT CATACAGGAG CAGCACCCTG AGGAGGAACT GATCAAGACT 
AAGCAGGATG AAGTCAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCTCT GCAGAGGCAG 
GGGAAGCTCT TTGGGGCAGC AGAAGTTCAG CGCTTTAACA GGGATGTGGA TGAGACTATC 
AGTTGGATTA AGGAAAAGGA GCAGTTAATG GCCTCTGATG ATTTTGGCCG AGACCTGGCA 
AGTGTTCAGG CTCTGCTTCG GAAGCACGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAA 
GACAAGGTCA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCCCA CCCTCTGAGT 
GCAACACAGA TTCAAGTGAA GCGAGAGGAA CTGATTACAA ACTGGGAGCA GATCCGCACC 
TTGGCGGCAG AGAGACATGC ACGGCTCAAT GATTCATACA GGCTTCAACG CTTCCTTGCT 
GACTTCCGTG ACCTCACCAG CTGGGTGACT GAGATGAAAG CCCTCATCAA TGCAGATGAG 
CTTGCCAGTG ATGTGGCTGG GGCTGAAGCC CTGCTAGATA GACACCAAGA GCACAAGGGT 
GAAATTGATG CCCATGAAGA CAGCTTCAAA TCTGCAGATG AATCTGGACA GGCACTGCTT 
GCTGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCGTCCT TTCCGAGGAG 
AGAGCGGCGC TGCTGGAGCT GTGGGAGCTG CGCAGGCAGC AGTACGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGGGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ATGAAGACTT GGGAGATTCC TTGGATAGTG TGGAAGCGCT TCTTAAGAAG 
CACGAAGACT TTGAGAAATC CCTTAGTGCC CAGGAGGAAA AGATTACAGC ATTAGATGAA 
TTTGCAACCA AGCTAATTCA GAACAACCAC TATGCAATGG AAGATGTGGC CACTCGCCGA 
GATGCTCTGT TGAGCCGCCG CAATGCCCTT CACGAGAGAG CCATGCGTCG CCGGGCCCAG 
CTAGCCGATT CTTTCCATCT GCAGCAGTTT TTCCGTGATT CTGATGAGCT CAAGAGTTGG 
GTCAATGAGA AGATGAAAAC TGCCACAGAT GAAGCTTATA AAGATCCATC CAACCTACAA 
GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CAGCAAACCA GAGCCGAATT 
GATGCCTTGG AGAAAGCTGG CCAAAAGCTG ATTGATGTCA ACCACTATGC CAAGGATGAA 
GTGGCAGCTC GTATGAATGA GGTGATCAGT TTGTGGAAGA AACTGCTAGA GGCCACTGAA 
CTGAAAGGAA TAAAGCTTCG TGAAGCCAAC CAGCAACAGC AATTTAATCG CAATGTTGAG 
GATATTGAAT TGTGGCTATA TGAAGTAGAA GGTCACTTGG CTTCGGATGA TTACGGCAAA 
GATCTTACCA ATGTGCAGAA CCTCCAGAAG AAACATGCAC TGCTAGAGGC AGATGTGGCT 
GCTCACCAGG ACCGAATTGA TGGCATCACC ATTCAGGCCC GCCAGTTCCA AGATGCTGGC 
CATTTTGATG CAGAAAACAT CAAGAAGAAA CAGGAAGCCC TCGTGGCTCG CTATGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCTCTGCG GTTGCAGCAG 
CTCTTCCGGG ATGTTGAGGA TGAGGAGACG TGGATTCGAG AGAAAGAGCC CATTGCCGCA 
TCTACCAACA GAGGTAAGGA TTTAATTGGG GTCCAGAATC TGCTAAAGAA ACATCAAGCC 
TTACAAGCAG AAATTGCTGG ACATGAACCA CGCATCAAAG CAGTTACACA GAAGGGGAAT 
GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAGCT TCACGAGCTG 
AACCAAAAGT GGGAGGCACT GAAAGCCAAA GCTTCCCAGC GTCGGCAGGA CCTGGAGGAC 
TCTCTGCAGG CCCAGCAGTA CTTTGCTGAT GCTAACGAGG CTGAATCCTG GATGCGGGAG 
AAGGAACCCA TTGTGGGCAG CACTGACTAT GGCAAGGACG AAGACTCTGC TGAGGCTCTA 
CTGAAGAAAC ACGAAGCTTT GATGTCAGAT CTCAGTGCCT ACGGCAGCAG CATCCAGGCT 
TTGCGAGAAC AAGCACAGTC CTGCCGGCAA CAAGTGGCCC CCACGGATGA TGAGACTGGG 
AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAGGGAG ATATCCTTAC CTTACTCAAC AGCACCAACA AGGATTGGTG GAAAGTGGAA 
GTGAACGATC GTCAGGGTTT TGTGCCGGCT GCGTACGTGA AGAAATTGGA CCCCGCCCAG 
TCAGCCTCCC GGGAGAATCT CCTGGAGGAG CAAGGCAGCA TAGCACTGCG GCAGGAGCAG 
ATTGACAATC AATATCATTC TCTGCTGGAA CTGGGTGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGTTGCA AGAAGTTTAT GTTGTTCCGT GAAGCGAATG AACTACAGCA ATGGATCAAT 
GAGAAGGAAG CCGCTCTGAC AAGTGAGGAG GTCGGAGCAG ACTTGGAGCA GGTTGAGGTG 
CTCCAGAAGA AGTTTGATGA CTTCCAGAAG GACCTGAAGG CCAATGAGTC ACGGTTGAAG 
GACATTAACA AGGTAGCTGA AGACCTGGAG TCTGAAGGTC TCATGGCAGA GGAGGTGCAG 
GCTGTGCAAC AACAGGAAGT GTATGGCATG ATGCCCAGGG ATGAAACTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGTCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTGA ATGAGCGCTG GCGGTCCCTA CAGCAGCTGG CCGAGGAACG GAGCCAGCTC 
TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CTGATGAAAC CAAAGAATGG 
ATTGAAGAGA AGAATCAAGC TCTAAACACA GACAATTATG GACATGATCT CGCCAGTGTC 
CAGGCCCTGC AACGCAAGCA TGAGGGCTTC GAGAGGGACC TTGCGGCTCT CGGTGACAAG 
GTAAACTCCC TTGGTGAAAC AGCAGAGCGC CTGATCCAGT CCCATCCCGA GTCAGCAGAA 
GACCTGCAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA GCAGCCTGGG GAAACGTGCA 
GATCAGCGCA AGGCAAAGTT GGGTGACTCC CACGACCTGC AGCGCTTCCT TAGCGATTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATA CGGGGGTTGG TGTCCTCAGA TGAGCTAGCC 
AAGGATGTCA CCGGAGCTGA GGCATTGCTG GAGCGACACC AGGAACACCG GACAGAAATC 
GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GACAGCAGCT GTTGGCTCAC 
GGACACTATG CCAGCCCTGA GATCAAGCAG AAACTTGATA TTCTTGACCA GGAGCGTGCA 
GACCTGGAGA AGGCCTGGGT TCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAACTGCAG 
CTGTTCCATC GGGACTGTGA GCAAGCTGAG AACTGGATGG CTGCCCGGGA GGCCTTCTTG 
AATACCGAAG ACAAAGGAGA CTCACTGGAC AGCGTAGAGG CTCTGATCAA AAAACATGAA 
GACTTTGACA AAGCGATTAA CGTCCAGGAA GAGAAGATTG CTGCTCTGCA GGCCTTTGCC 
GACCAGCTCA TCGCTGCCGG CCATTATGCC AAGGGAGACA TTTCTAGCCG GCGCAATGAG 
GTCTTGGACA GGTGGCGACG TCTGAAAGCC CAGATGATTG AGAAAAGGTC AAAGCTAGGA 
GAATCTCAAA CCCTCCAACA GTTCAGCCGG GATGTGGATG AGATTGAGGC TTGGATCAGT 
GAAAAATTGC AAACAGCGAG TGATGAGTCG TACAAGGATC CCACCAACAT CCAGCTTTCC 
AAGCTGCTGA GCAAGCACCA GAAGCACCAG GCTTTTGAAG CAGAGCTGCA TGCCAACGCT 
GACCGGATCC GTGGGGTTAT CGACATGGGC AACTCCCTCA TTGAACGTGG AGCCTGTGCC 
GGCAGTGAGG ATGCTGTCAA GGCCCGCCTG GCTGCCTTAG CTGACCAGTG GCAGTTCTTG 
GTGCAAAAGT CAGCGGAAAA GAGCCAGAAA CTGAAAGAAG CCAACAAGCA GCAGAACTTC 
AACACAGGGA TCAAGGACTT TGACTTCTGG CTGTCTGAGG TGGAGGCCCT GCTGGCATCC 
GAAGATTATG GCAAAGACCT AGCTTCTGTG AACAACCTGC TGAAAAAGCA TCAACTGCTG 
GAAGCAGATA TATCTGCCCA TGAGGATCGC CTGAAGGACC TGAACAGCCA GGCAGACAGC 
CTGATGACCA GCAGTGCCTT CGACACCTCC CAAGTAAAGG ACAAGAGGGA CACCATCAAC 
GGGCGCTTCC AGAAGATCAA GAGCATGGCG GCCTCCCGGC GAGCCAAGCT GAATGAATCC 
CATCGCCTGC ACCAGTTCTT CCGGGACATG GATGACGAGG AGTCCTGGAT CAAGGAGAAG 
AAGCTGCTGG TGGGCTCAGA GGACTACGGC CGGGACCTAA CCGGCGTGCA GAACCTGAGG 
AAGAAGCACA AGCGGCTGGA AGCAGAACTG GCTGCGCATG AGCCGGCTAT TCAGGGTGTC 
CTGGACACTG GCAAGAAGCT GTCCGATGAC AACACCATCG GGAAAGAGGA GATCCAGCAG 
CGGCTGGCGC AGTTTGTGGA GCACTGGAAA GAGCTGAAGC AGCTGGCAGC TGCCCGGGGT 
CAGCGGCTGG AAGAGTCCTT GGAATATCAG CAGTTTGTAG CCAATGTGGA AGAGGAAGAA 
GCCTGGATCA ATGAGAAAAT GACCCTGGTG GCCAGCGAAG ATTATGGCGA CACTCTTGCC 
GCCATCCAGG GCTTACTGAA GAAACATGAA GCTTTTGAGA CAGACTTCAC CGTCCACAAG 
GATCGCGTGA ATGATGTCTG CACCAATGGA CAAGACCTCA TTAAGAAGAA CAATCACCAT 
GAGGAGAACA TCTCTTCAAA GATGAAGGGC CTGAACGGGA AAGTGTCAGA CCTGGAGAAA 
GCTGCAGCCC AGAGAAAGGC GAAGCTGGAT GAGAACTCGG CCTTCCTTCA GTTCAACTGG 
AAGGCGGACG TGGTGGAGTC CTGGATCGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT 
TATGGCCGAG ACCTGTCTTC TGTGCAGACG CTCCTCACCA AACAGGAAAC TTTTGACGCT 
GGGCTGCAGG CCTTCCAGCA GGAAGGCATT GCCAACATCA CTGCCCTCAA AGATCAGCTT 
CTCGCCGCCA AACACGTTCA GTCCAAGGCC ATCGAGGCCC GGCACGCCTC CCTCATGAAG 
AGGTGGAGCC AGCTTCTGGC CAACTCAGCC GCCCGCAAGA AGAAGCTTCT GGAGGCTCAG 
AGTCACTTCC GCAAGGTGGA GGACCTCTTC CTGACCTTCG CCAAAAAGGC TTCTGCCTTC 
AACAGCTGGT TTGAAAATGC AGAGGAGGAC TTAACAGACC CCGTGCGCTG CAACTCCTTG 
GAAGAAATCA AAGCTTTGCG CGAGGCCCAC GACGCCTTCC GCTCCTCCCT CAGCTCTGCC 
CAGGCTGACT TCAACCAGCT GGCCGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTAGCC 
TCCAACCCCT ACACCTGGTT TACCATGGAG GCCCTGGAGG AGACCTGGAG GAACCTACAG 
AAAATCATCA AGGAGAGGGA GCTGGAGCTG CAGAAGGAAC AGCGGCGGCA GGAGGAGAAC 
GACAAGCTGC GCCAGGAGTT TGCCCAGCAC GCCAACGCCT TCCACCAGTG GATCCAAGAG 
ACCAGGTCCT GTATGGTGGA AGAGTCGGGG ACCCTCGAAT CCCAGCTTGA AGCTACCAAA 
CGCAAGCACC AGGAAATCCG AGCCATGAGA AGTCAGCTCA AAAAGATCGA GGACCTGGGG 
GCCGCCATGG AGGAGGCCCT CATCCTGGAC AACAAGTACA CGGAGCACAG CACCGTGGGC 
CTCGCCCAGC AGTGGGACCA GCTGGACCAG CTGGGCATGC GCATGCAGCA CAACCTGGAG 
CAGCAGATCC AGGCCAGGAA CACAACAGGT GTGACTGAGG AGGCCCTCAA AGAATTCAGC 
ATGATGTTTA AACACTTTGA CAAGGACAAG TCTGGCAGGC TGAACCATCA GGAGTTCAAA 
TCTTGCCTGC GCTCCCTGGG CTATGACCTG CCCATGGTGG AGGAAGGGGA ACCTGACCCT 
GAGTTCGAGG CAATCCTGGA CACGGTGGAT CCGAACAGAG ATGGCCATGT CTCCTTGCAA 
GAATACATGG CTTTCATGAT CAGCCGCGAA ACTGAGAACG TCAAGTCCAG CGAGGAGATT 
GAGAGCGCCT TCCGGGCCCT CAGCTCAGAG GGAAAGCCTT ACGTGACCAA GGAGGAGCTC 
TACCAGAACC TGACCCGGGA ACAAGCCGAC TACTGCGTCT CCCACATGAA GCCCTACGTG 
GACGGCAAGG GCCGCGAGCT CCCCACCGCG TTCGACTACG TGGAGTTCAC CCGCTCGCTT 
TTCGTGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016870549.1
CDS	14..7405
Translation	MLSSFRKRRVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELASDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMRRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPTDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGMMPRDETDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKQKLDILDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAAGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINGRFQKIKSMAASRRAKLNESHRLHQFFRDMDDEESWIKEKKLLVGSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHASLMKRWSQLLANSAARKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_017015060.1
Organism	Homo sapiens(human)
Definition	Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017015060.1

ORF Insert Sequence:

ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACCG ATACCACCGC 
TTCAAGGAAC TCTCAACCCT TAGGCGTCAG AAGCTGGAAG ATTCCTATCG ATTCCAGTTC 
TTTCAAAGAG ATGCTGAAGA GCTGGAGAAA TGGATACAGG AAAAACTTCA GATTGCATCT 
GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGAAAGC TTCAGAAGCA TCAAGCATTT 
GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAGC TGGATGAAAC TGGAAACCTG 
ATGATCTCAG AAGGGCATTT TGCATCTGAA ACCATACGGA CCCGTTTGAT GGAGCTGCAC 
CGCCAGTGGG AATTACTTTT GGAGAAGATG CGAGAAAAAG GAATCAAACT GCTGCAGGCC 
CAGAAGTTGG TGCAGTACTT ACGAGAATGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCAATTG TTACTTCTGA AGAGCTGGGC CAGGATCTGG AGCATGTAGA GGTTTTACAG 
AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCTCATG AAGAAAGAGT TAATGAAGTG 
AACCAGTTTG CTGCCAAACT CATACAGGAG CAGCACCCTG AGGAGGAACT GATCAAGACT 
AAGCAGGATG AAGTCAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCTCT GCAGAGGCAG 
GGGAAGCTCT TTGGGGCAGC AGAAGTTCAG CGCTTTAACA GGGATGTGGA TGAGACTATC 
AGTTGGATTA AGGAAAAGGA GCAGTTAATG GCCTCTGATG ATTTTGGCCG AGACCTGGCA 
AGTGTTCAGG CTCTGCTTCG GAAGCACGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAA 
GACAAGGTCA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCCCA CCCTCTGAGT 
GCAACACAGA TTCAAGTGAA GCGAGAGGAA CTGATTACAA ACTGGGAGCA GATCCGCACC 
TTGGCGGCAG AGAGACATGC ACGGCTCAAT GATTCATACA GGCTTCAACG CTTCCTTGCT 
GACTTCCGTG ACCTCACCAG CTGGGTGACT GAGATGAAAG CCCTCATCAA TGCAGATGAG 
CTTGCCAGTG ATGTGGCTGG GGCTGAAGCC CTGCTAGATA GACACCAAGA GCACAAGGGT 
GAAATTGATG CCCATGAAGA CAGCTTCAAA TCTGCAGATG AATCTGGACA GGCACTGCTT 
GCTGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCGTCCT TTCCGAGGAG 
AGAGCGGCGC TGCTGGAGCT GTGGGAGCTG CGCAGGCAGC AGTACGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGGGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ATGAAGACTT GGGAGATTCC TTGGATAGTG TGGAAGCGCT TCTTAAGAAG 
CACGAAGACT TTGAGAAATC CCTTAGTGCC CAGGAGGAAA AGATTACAGC ATTAGATGAA 
TTTGCAACCA AGCTAATTCA GAACAACCAC TATGCAATGG AAGATGTGGC CACTCGCCGA 
GATGCTCTGT TGAGCCGCCG CAATGCCCTT CACGAGAGAG CCATGCGTCG CCGGGCCCAG 
CTAGCCGATT CTTTCCATCT GCAGCAGTTT TTCCGTGATT CTGATGAGCT CAAGAGTTGG 
GTCAATGAGA AGATGAAAAC TGCCACAGAT GAAGCTTATA AAGATCCATC CAACCTACAA 
GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CAGCAAACCA GAGCCGAATT 
GATGCCTTGG AGAAAGCTGG CCAAAAGCTG ATTGATGTCA ACCACTATGC CAAGGATGAA 
GTGGCAGCTC GTATGAATGA GGTGATCAGT TTGTGGAAGA AACTGCTAGA GGCCACTGAA 
CTGAAAGGAA TAAAGCTTCG TGAAGCCAAC CAGCAACAGC AATTTAATCG CAATGTTGAG 
GATATTGAAT TGTGGCTATA TGAAGTAGAA GGTCACTTGG CTTCGGATGA TTACGGCAAA 
GATCTTACCA ATGTGCAGAA CCTCCAGAAG AAACATGCAC TGCTAGAGGC AGATGTGGCT 
GCTCACCAGG ACCGAATTGA TGGCATCACC ATTCAGGCCC GCCAGTTCCA AGATGCTGGC 
CATTTTGATG CAGAAAACAT CAAGAAGAAA CAGGAAGCCC TCGTGGCTCG CTATGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCTCTGCG GTTGCAGCAG 
CTCTTCCGGG ATGTTGAGGA TGAGGAGACG TGGATTCGAG AGAAAGAGCC CATTGCCGCA 
TCTACCAACA GAGGTAAGGA TTTAATTGGG GTCCAGAATC TGCTAAAGAA ACATCAAGCC 
TTACAAGCAG AAATTGCTGG ACATGAACCA CGCATCAAAG CAGTTACACA GAAGGGGAAT 
GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAGCT TCACGAGCTG 
AACCAAAAGT GGGAGGCACT GAAAGCCAAA GCTTCCCAGC GTCGGCAGGA CCTGGAGGAC 
TCTCTGCAGG CCCAGCAGTA CTTTGCTGAT GCTAACGAGG CTGAATCCTG GATGCGGGAG 
AAGGAACCCA TTGTGGGCAG CACTGACTAT GGCAAGGACG AAGACTCTGC TGAGGCTCTA 
CTGAAGAAAC ACGAAGCTTT GATGTCAGAT CTCAGTGCCT ACGGCAGCAG CATCCAGGCT 
TTGCGAGAAC AAGCACAGTC CTGCCGGCAA CAAGTGGCCC CCACGGATGA TGAGACTGGG 
AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAGGGAG ATATCCTTAC CTTACTCAAC AGCACCAACA AGGATTGGTG GAAAGTGGAA 
GTGAACGATC GTCAGGGTTT TGTGCCGGCT GCGTACGTGA AGAAATTGGA CCCCGCCCAG 
TCAGCCTCCC GGGAGAATCT CCTGGAGGAG CAAGGCAGCA TAGCACTGCG GCAGGAGCAG 
ATTGACAATC AATATCATTC TCTGCTGGAA CTGGGTGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGTTGCA AGAAGTTTAT GTTGTTCCGT GAAGCGAATG AACTACAGCA ATGGATCAAT 
GAGAAGGAAG CCGCTCTGAC AAGTGAGGAG GTCGGAGCAG ACTTGGAGCA GGTTGAGGTG 
CTCCAGAAGA AGTTTGATGA CTTCCAGAAG GACCTGAAGG CCAATGAGTC ACGGTTGAAG 
GACATTAACA AGGTAGCTGA AGACCTGGAG TCTGAAGGTC TCATGGCAGA GGAGGTGCAG 
GCTGTGCAAC AACAGGAAGT GTATGGCATG ATGCCCAGGG ATGAAACTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGTCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTGA ATGAGCGCTG GCGGTCCCTA CAGCAGCTGG CCGAGGAACG GAGCCAGCTC 
TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CTGATGAAAC CAAAGAATGG 
ATTGAAGAGA AGAATCAAGC TCTAAACACA GACAATTATG GACATGATCT CGCCAGTGTC 
CAGGCCCTGC AACGCAAGCA TGAGGGCTTC GAGAGGGACC TTGCGGCTCT CGGTGACAAG 
GTAAACTCCC TTGGTGAAAC AGCAGAGCGC CTGATCCAGT CCCATCCCGA GTCAGCAGAA 
GACCTGCAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA GCAGCCTGGG GAAACGTGCA 
GATCAGCGCA AGGCAAAGTT GGGTGACTCC CACGACCTGC AGCGCTTCCT TAGCGATTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATA CGGGGGTTGG TGTCCTCAGA TGAGCTAGCC 
AAGGATGTCA CCGGAGCTGA GGCATTGCTG GAGCGACACC AGGAACACCG GACAGAAATC 
GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GACAGCAGCT GTTGGCTCAC 
GGACACTATG CCAGCCCTGA GATCAAGCAG AAACTTGATA TTCTTGACCA GGAGCGTGCA 
GACCTGGAGA AGGCCTGGGT TCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAACTGCAG 
CTGTTCCATC GGGACTGTGA GCAAGCTGAG AACTGGATGG CTGCCCGGGA GGCCTTCTTG 
AATACCGAAG ACAAAGGAGA CTCACTGGAC AGCGTAGAGG CTCTGATCAA AAAACATGAA 
GACTTTGACA AAGCGATTAA CGTCCAGGAA GAGAAGATTG CTGCTCTGCA GGCCTTTGCC 
GACCAGCTCA TCGCTGCCGG CCATTATGCC AAGGGAGACA TTTCTAGCCG GCGCAATGAG 
GTCTTGGACA GGTGGCGACG TCTGAAAGCC CAGATGATTG AGAAAAGGTC AAAGCTAGGA 
GAATCTCAAA CCCTCCAACA GTTCAGCCGG GATGTGGATG AGATTGAGGC TTGGATCAGT 
GAAAAATTGC AAACAGCGAG TGATGAGTCG TACAAGGATC CCACCAACAT CCAGCTTTCC 
AAGCTGCTGA GCAAGCACCA GAAGCACCAG GCTTTTGAAG CAGAGCTGCA TGCCAACGCT 
GACCGGATCC GTGGGGTTAT CGACATGGGC AACTCCCTCA TTGAACGTGG AGCCTGTGCC 
GGCAGTGAGG ATGCTGTCAA GGCCCGCCTG GCTGCCTTAG CTGACCAGTG GCAGTTCTTG 
GTGCAAAAGT CAGCGGAAAA GAGCCAGAAA CTGAAAGAAG CCAACAAGCA GCAGAACTTC 
AACACAGGGA TCAAGGACTT TGACTTCTGG CTGTCTGAGG TGGAGGCCCT GCTGGCATCC 
GAAGATTATG GCAAAGACCT AGCTTCTGTG AACAACCTGC TGAAAAAGCA TCAACTGCTG 
GAAGCAGATA TATCTGCCCA TGAGGATCGC CTGAAGGACC TGAACAGCCA GGCAGACAGC 
CTGATGACCA GCAGTGCCTT CGACACCTCC CAAGTAAAGG ACAAGAGGGA CACCATCAAC 
GGGCGCTTCC AGAAGATCAA GAGCATGGCG GCCTCCCGGC GAGCCAAGCT GAATGAATCC 
CATCGCCTGC ACCAGTTCTT CCGGGACATG GATGACGAGG AGTCCTGGAT CAAGGAGAAG 
AAGCTGCTGG TGGGCTCAGA GGACTACGGC CGGGACCTAA CCGGCGTGCA GAACCTGAGG 
AAGAAGCACA AGCGGCTGGA AGCAGAACTG GCTGCGCATG AGCCGGCTAT TCAGGGTGTC 
CTGGACACTG GCAAGAAGCT GTCCGATGAC AACACCATCG GGAAAGAGGA GATCCAGCAG 
CGGCTGGCGC AGTTTGTGGA GCACTGGAAA GAGCTGAAGC AGCTGGCAGC TGCCCGGGGT 
CAGCGGCTGG AAGAGTCCTT GGAATATCAG CAGTTTGTAG CCAATGTGGA AGAGGAAGAA 
GCCTGGATCA ATGAGAAAAT GACCCTGGTG GCCAGCGAAG ATTATGGCGA CACTCTTGCC 
GCCATCCAGG GCTTACTGAA GAAACATGAA GCTTTTGAGA CAGACTTCAC CGTCCACAAG 
GATCGCGTGA ATGATGTCTG CACCAATGGA CAAGACCTCA TTAAGAAGAA CAATCACCAT 
GAGGAGAACA TCTCTTCAAA GATGAAGGGC CTGAACGGGA AAGTGTCAGA CCTGGAGAAA 
GCTGCAGCCC AGAGAAAGGC GAAGCTGGAT GAGAACTCGG CCTTCCTTCA GTTCAACTGG 
AAGGCGGACG TGGTGGAGTC CTGGATCGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT 
TATGGCCGAG ACCTGTCTTC TGTGCAGACG CTCCTCACCA AACAGGAAAC TTTTGACGCT 
GGGCTGCAGG CCTTCCAGCA GGAAGGCATT GCCAACATCA CTGCCCTCAA AGATCAGCTT 
CTCGCCGCCA AACACGTTCA GTCCAAGGCC ATCGAGGCCC GGCACGCCTC CCTCATGAAG 
AGGTGGAGCC AGCTTCTGGC CAACTCAGCC GCCCGCAAGA AGAAGCTTCT GGAGGCTCAG 
AGTCACTTCC GCAAGGTGGA GGACCTCTTC CTGACCTTCG CCAAAAAGGC TTCTGCCTTC 
AACAGCTGGT TTGAAAATGC AGAGGAGGAC TTAACAGACC CCGTGCGCTG CAACTCCTTG 
GAAGAAATCA AAGCTTTGCG CGAGGCCCAC GACGCCTTCC GCTCCTCCCT CAGCTCTGCC 
CAGGCTGACT TCAACCAGCT GGCCGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTAGCC 
TCCAACCCCT ACACCTGGTT TACCATGGAG GCCCTGGAGG AGACCTGGAG GAACCTACAG 
AAAATCATCA AGGAGAGGGA GCTGGAGCTG CAGAAGGAAC AGCGGCGGCA GGAGGAGAAC 
GACAAGCTGC GCCAGGAGTT TGCCCAGCAC GCCAACGCCT TCCACCAGTG GATCCAAGAG 
ACCAGGTCCT GTATGGTGGA AGAGTCGGGG ACCCTCGAAT CCCAGCTTGA AGCTACCAAA 
CGCAAGCACC AGGAAATCCG AGCCATGAGA AGTCAGCTCA AAAAGATCGA GGACCTGGGG 
GCCGCCATGG AGGAGGCCCT CATCCTGGAC AACAAGTACA CGGAGCACAG CACCGTGGGC 
CTCGCCCAGC AGTGGGACCA GCTGGACCAG CTGGGCATGC GCATGCAGCA CAACCTGGAG 
CAGCAGATCC AGGCCAGGAA CACAACAGGT GTGACTGAGG AGGCCCTCAA AGAATTCAGC 
ATGATGTTTA AACACTTTGA CAAGGACAAG TCTGGCAGGC TGAACCATCA GGAGTTCAAA 
TCTTGCCTGC GCTCCCTGGG CTATGACCTG CCCATGGTGG AGGAAGGGGA ACCTGACCCT 
GAGTTCGAGG CAATCCTGGA CACGGTGGAT CCGAACAGAG ATGGCCATGT CTCCTTGCAA 
GAATACATGG CTTTCATGAT CAGCCGCGAA ACTGAGAACG TCAAGTCCAG CGAGGAGATT 
GAGAGCGCCT TCCGGGCCCT CAGCTCAGAG GGAAAGCCTT ACGTGACCAA GGAGGAGCTC 
TACCAGAACC TGACCCGGGA ACAAGCCGAC TACTGCGTCT CCCACATGAA GCCCTACGTG 
GACGGCAAGG GCCGCGAGCT CCCCACCGCG TTCGACTACG TGGAGTTCAC CCGCTCGCTT 
TTCGTGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_017015061.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016870550.1 Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X13, mRNA.

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CloneID	OHu98857
Clone ID Related Accession (Same CDS sequence)	NM_001363765.2 , NM_001375314.2 , XM_017015061.1 , NM_001363765.1
Accession Version	XM_017015061.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAA GTGGGGTCAA AGTGCTGGAA ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTAG ACCGATACCA CCGCTTCAAG GAACTCTCAA CCCTTAGGCG TCAGAAGCTG 
GAAGATTCCT ATCGATTCCA GTTCTTTCAA AGAGATGCTG AAGAGCTGGA GAAATGGATA 
CAGGAAAAAC TTCAGATTGC ATCTGATGAG AATTATAAAG ACCCAACCAA CTTGCAGGGA 
AAGCTTCAGA AGCATCAAGC ATTTGAAGCT GAAGTGCAGG CCAACTCAGG AGCCATTGTT 
AAGCTGGATG AAACTGGAAA CCTGATGATC TCAGAAGGGC ATTTTGCATC TGAAACCATA 
CGGACCCGTT TGATGGAGCT GCACCGCCAG TGGGAATTAC TTTTGGAGAA GATGCGAGAA 
AAAGGAATCA AACTGCTGCA GGCCCAGAAG TTGGTGCAGT ACTTACGAGA ATGTGAGGAC 
GTGATGGACT GGATCAATGA CAAGGAAGCA ATTGTTACTT CTGAAGAGCT GGGCCAGGAT 
CTGGAGCATG TAGAGGTTTT ACAGAAGAAA TTTGAAGAGT TTCAAACAGA TATGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTGAACCAG TTTGCTGCCA AACTCATACA GGAGCAGCAC 
CCTGAGGAGG AACTGATCAA GACTAAGCAG GATGAAGTCA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CTCTGCAGAG GCAGGGGAAG CTCTTTGGGG CAGCAGAAGT TCAGCGCTTT 
AACAGGGATG TGGATGAGAC TATCAGTTGG ATTAAGGAAA AGGAGCAGTT AATGGCCTCT 
GATGATTTTG GCCGAGACCT GGCAAGTGTT CAGGCTCTGC TTCGGAAGCA CGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAAGACAAG GTCAAAGCCC TGTGTGCTGA GGCTGACCGC 
CTGCAACAGT CCCACCCTCT GAGTGCAACA CAGATTCAAG TGAAGCGAGA GGAACTGATT 
ACAAACTGGG AGCAGATCCG CACCTTGGCG GCAGAGAGAC ATGCACGGCT CAATGATTCA 
TACAGGCTTC AACGCTTCCT TGCTGACTTC CGTGACCTCA CCAGCTGGGT GACTGAGATG 
AAAGCCCTCA TCAATGCAGA TGAGCTTGCC AGTGATGTGG CTGGGGCTGA AGCCCTGCTA 
GATAGACACC AAGAGCACAA GGGTGAAATT GATGCCCATG AAGACAGCTT CAAATCTGCA 
GATGAATCTG GACAGGCACT GCTTGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGACCG TCCTTTCCGA GGAGAGAGCG GCGCTGCTGG AGCTGTGGGA GCTGCGCAGG 
CAGCAGTACG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GGGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAATGAAG ACTTGGGAGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTTAA GAAGCACGAA GACTTTGAGA AATCCCTTAG TGCCCAGGAG 
GAAAAGATTA CAGCATTAGA TGAATTTGCA ACCAAGCTAA TTCAGAACAA CCACTATGCA 
ATGGAAGATG TGGCCACTCG CCGAGATGCT CTGTTGAGCC GCCGCAATGC CCTTCACGAG 
AGAGCCATGC GTCGCCGGGC CCAGCTAGCC GATTCTTTCC ATCTGCAGCA GTTTTTCCGT 
GATTCTGATG AGCTCAAGAG TTGGGTCAAT GAGAAGATGA AAACTGCCAC AGATGAAGCT 
TATAAAGATC CATCCAACCT ACAAGGAAAA GTACAGAAGC ATCAGGCTTT TGAGGCTGAG 
CTCTCAGCAA ACCAGAGCCG AATTGATGCC TTGGAGAAAG CTGGCCAAAA GCTGATTGAT 
GTCAACCACT ATGCCAAGGA TGAAGTGGCA GCTCGTATGA ATGAGGTGAT CAGTTTGTGG 
AAGAAACTGC TAGAGGCCAC TGAACTGAAA GGAATAAAGC TTCGTGAAGC CAACCAGCAA 
CAGCAATTTA ATCGCAATGT TGAGGATATT GAATTGTGGC TATATGAAGT AGAAGGTCAC 
TTGGCTTCGG ATGATTACGG CAAAGATCTT ACCAATGTGC AGAACCTCCA GAAGAAACAT 
GCACTGCTAG AGGCAGATGT GGCTGCTCAC CAGGACCGAA TTGATGGCAT CACCATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTT GATGCAGAAA ACATCAAGAA GAAACAGGAA 
GCCCTCGTGG CTCGCTATGA GGCACTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCCGATTCTC TGCGGTTGCA GCAGCTCTTC CGGGATGTTG AGGATGAGGA GACGTGGATT 
CGAGAGAAAG AGCCCATTGC CGCATCTACC AACAGAGGTA AGGATTTAAT TGGGGTCCAG 
AATCTGCTAA AGAAACATCA AGCCTTACAA GCAGAAATTG CTGGACATGA ACCACGCATC 
AAAGCAGTTA CACAGAAGGG GAATGCCATG GTGGAGGAAG GCCATTTTGC TGCAGAGGAT 
GTGAAGGCCA AGCTTCACGA GCTGAACCAA AAGTGGGAGG CACTGAAAGC CAAAGCTTCC 
CAGCGTCGGC AGGACCTGGA GGACTCTCTG CAGGCCCAGC AGTACTTTGC TGATGCTAAC 
GAGGCTGAAT CCTGGATGCG GGAGAAGGAA CCCATTGTGG GCAGCACTGA CTATGGCAAG 
GACGAAGACT CTGCTGAGGC TCTACTGAAG AAACACGAAG CTTTGATGTC AGATCTCAGT 
GCCTACGGCA GCAGCATCCA GGCTTTGCGA GAACAAGCAC AGTCCTGCCG GCAACAAGTG 
GCCCCCACGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CTCTCTACGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAG GGAGATATCC TTACCTTACT CAACAGCACC 
AACAAGGATT GGTGGAAAGT GGAAGTGAAC GATCGTCAGG GTTTTGTGCC GGCTGCGTAC 
GTGAAGAAAT TGGACCCCGC CCAGTCAGCC TCCCGGGAGA ATCTCCTGGA GGAGCAAGGC 
AGCATAGCAC TGCGGCAGGA GCAGATTGAC AATCAATATC ATTCTCTGCT GGAACTGGGT 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TTATGTTGTT CCGTGAAGCG 
AATGAACTAC AGCAATGGAT CAATGAGAAG GAAGCCGCTC TGACAAGTGA GGAGGTCGGA 
GCAGACTTGG AGCAGGTTGA GGTGCTCCAG AAGAAGTTTG ATGACTTCCA GAAGGACCTG 
AAGGCCAATG AGTCACGGTT GAAGGACATT AACAAGGTAG CTGAAGACCT GGAGTCTGAA 
GGTCTCATGG CAGAGGAGGT GCAGGCTGTG CAACAACAGG AAGTGTATGG CATGATGCCC 
AGGGATGAAA CTGATTCCAA GACAGCCTCC CCGTGGAAGT CTGCTCGTCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTACAGCAG 
CTGGCCGAGG AACGGAGCCA GCTCTTGGGC AGCGCCCATG AAGTACAGAG GTTCCACAGA 
GATGCTGATG AAACCAAAGA ATGGATTGAA GAGAAGAATC AAGCTCTAAA CACAGACAAT 
TATGGACATG ATCTCGCCAG TGTCCAGGCC CTGCAACGCA AGCATGAGGG CTTCGAGAGG 
GACCTTGCGG CTCTCGGTGA CAAGGTAAAC TCCCTTGGTG AAACAGCAGA GCGCCTGATC 
CAGTCCCATC CCGAGTCAGC AGAAGACCTG CAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGAGCAGCC TGGGGAAACG TGCAGATCAG CGCAAGGCAA AGTTGGGTGA CTCCCACGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGGGGG 
TTGGTGTCCT CAGATGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCATT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATCGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGACAGC AGCTGTTGGC TCACGGACAC TATGCCAGCC CTGAGATCAA GCAGAAACTT 
GATATTCTTG ACCAGGAGCG TGCAGACCTG GAGAAGGCCT GGGTTCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAACT GCAGCTGTTC CATCGGGACT GTGAGCAAGC TGAGAACTGG 
ATGGCTGCCC GGGAGGCCTT CTTGAATACC GAAGACAAAG GAGACTCACT GGACAGCGTA 
GAGGCTCTGA TCAAAAAACA TGAAGACTTT GACAAAGCGA TTAACGTCCA GGAAGAGAAG 
ATTGCTGCTC TGCAGGCCTT TGCCGACCAG CTCATCGCTG CCGGCCATTA TGCCAAGGGA 
GACATTTCTA GCCGGCGCAA TGAGGTCTTG GACAGGTGGC GACGTCTGAA AGCCCAGATG 
ATTGAGAAAA GGTCAAAGCT AGGAGAATCT CAAACCCTCC AACAGTTCAG CCGGGATGTG 
GATGAGATTG AGGCTTGGAT CAGTGAAAAA TTGCAAACAG CGAGTGATGA GTCGTACAAG 
GATCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ACCAGAAGCA CCAGGCTTTT 
GAAGCAGAGC TGCATGCCAA CGCTGACCGG ATCCGTGGGG TTATCGACAT GGGCAACTCC 
CTCATTGAAC GTGGAGCCTG TGCCGGCAGT GAGGATGCTG TCAAGGCCCG CCTGGCTGCC 
TTAGCTGACC AGTGGCAGTT CTTGGTGCAA AAGTCAGCGG AAAAGAGCCA GAAACTGAAA 
GAAGCCAACA AGCAGCAGAA CTTCAACACA GGGATCAAGG ACTTTGACTT CTGGCTGTCT 
GAGGTGGAGG CCCTGCTGGC ATCCGAAGAT TATGGCAAAG ACCTAGCTTC TGTGAACAAC 
CTGCTGAAAA AGCATCAACT GCTGGAAGCA GATATATCTG CCCATGAGGA TCGCCTGAAG 
GACCTGAACA GCCAGGCAGA CAGCCTGATG ACCAGCAGTG CCTTCGACAC CTCCCAAGTA 
AAGGACAAGA GGGACACCAT CAACGGGCGC TTCCAGAAGA TCAAGAGCAT GGCGGCCTCC 
CGGCGAGCCA AGCTGAATGA ATCCCATCGC CTGCACCAGT TCTTCCGGGA CATGGATGAC 
GAGGAGTCCT GGATCAAGGA GAAGAAGCTG CTGGTGGGCT CAGAGGACTA CGGCCGGGAC 
CTAACCGGCG TGCAGAACCT GAGGAAGAAG CACAAGCGGC TGGAAGCAGA ACTGGCTGCG 
CATGAGCCGG CTATTCAGGG TGTCCTGGAC ACTGGCAAGA AGCTGTCCGA TGACAACACC 
ATCGGGAAAG AGGAGATCCA GCAGCGGCTG GCGCAGTTTG TGGAGCACTG GAAAGAGCTG 
AAGCAGCTGG CAGCTGCCCG GGGTCAGCGG CTGGAAGAGT CCTTGGAATA TCAGCAGTTT 
GTAGCCAATG TGGAAGAGGA AGAAGCCTGG ATCAATGAGA AAATGACCCT GGTGGCCAGC 
GAAGATTATG GCGACACTCT TGCCGCCATC CAGGGCTTAC TGAAGAAACA TGAAGCTTTT 
GAGACAGACT TCACCGTCCA CAAGGATCGC GTGAATGATG TCTGCACCAA TGGACAAGAC 
CTCATTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CAAAGATGAA GGGCCTGAAC 
GGGAAAGTGT CAGACCTGGA GAAAGCTGCA GCCCAGAGAA AGGCGAAGCT GGATGAGAAC 
TCGGCCTTCC TTCAGTTCAA CTGGAAGGCG GACGTGGTGG AGTCCTGGAT CGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGC CGAGACCTGT CTTCTGTGCA GACGCTCCTC 
ACCAAACAGG AAACTTTTGA CGCTGGGCTG CAGGCCTTCC AGCAGGAAGG CATTGCCAAC 
ATCACTGCCC TCAAAGATCA GCTTCTCGCC GCCAAACACG TTCAGTCCAA GGCCATCGAG 
GCCCGGCACG CCTCCCTCAT GAAGAGGTGG AGCCAGCTTC TGGCCAACTC AGCCGCCCGC 
AAGAAGAAGC TTCTGGAGGC TCAGAGTCAC TTCCGCAAGG TGGAGGACCT CTTCCTGACC 
TTCGCCAAAA AGGCTTCTGC CTTCAACAGC TGGTTTGAAA ATGCAGAGGA GGACTTAACA 
GACCCCGTGC GCTGCAACTC CTTGGAAGAA ATCAAAGCTT TGCGCGAGGC CCACGACGCC 
TTCCGCTCCT CCCTCAGCTC TGCCCAGGCT GACTTCAACC AGCTGGCCGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT AGCCTCCAAC CCCTACACCT GGTTTACCAT GGAGGCCCTG 
GAGGAGACCT GGAGGAACCT ACAGAAAATC ATCAAGGAGA GGGAGCTGGA GCTGCAGAAG 
GAACAGCGGC GGCAGGAGGA GAACGACAAG CTGCGCCAGG AGTTTGCCCA GCACGCCAAC 
GCCTTCCACC AGTGGATCCA AGAGACCAGG ACATACCTCC TCGATGGGTC CTGTATGGTG 
GAAGAGTCGG GGACCCTCGA ATCCCAGCTT GAAGCTACCA AACGCAAGCA CCAGGAAATC 
CGAGCCATGA GAAGTCAGCT CAAAAAGATC GAGGACCTGG GGGCCGCCAT GGAGGAGGCC 
CTCATCCTGG ACAACAAGTA CACGGAGCAC AGCACCGTGG GCCTCGCCCA GCAGTGGGAC 
CAGCTGGACC AGCTGGGCAT GCGCATGCAG CACAACCTGG AGCAGCAGAT CCAGGCCAGG 
AACACAACAG GTGTGACTGA GGAGGCCCTC AAAGAATTCA GCATGATGTT TAAACACTTT 
GACAAGGACA AGTCTGGCAG GCTGAACCAT CAGGAGTTCA AATCTTGCCT GCGCTCCCTG 
GGCTATGACC TGCCCATGGT GGAGGAAGGG GAACCTGACC CTGAGTTCGA GGCAATCCTG 
GACACGGTGG ATCCGAACAG AGATGGCCAT GTCTCCTTGC AAGAATACAT GGCTTTCATG 
ATCAGCCGCG AAACTGAGAA CGTCAAGTCC AGCGAGGAGA TTGAGAGCGC CTTCCGGGCC 
CTCAGCTCAG AGGGAAAGCC TTACGTGACC AAGGAGGAGC TCTACCAGAA CCTGACCCGG 
GAACAAGCCG ACTACTGCGT CTCCCACATG AAGCCCTACG TGGACGGCAA GGGCCGCGAG 
CTCCCCACCG CGTTCGACTA CGTGGAGTTC ACCCGCTCGC TTTTCGTGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016870550.1
CDS	108..7481
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELASDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMRRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPTDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGMMPRDETDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKQKLDILDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAAGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINGRFQKIKSMAASRRAKLNESHRLHQFFRDMDDEESWIKEKKLLVGSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHASLMKRWSQLLANSAARKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_017015061.1
Organism	Homo sapiens(human)
Definition	Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017015061.1

ORF Insert Sequence:

ATGGACCCAA GTGGGGTCAA AGTGCTGGAA ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTAG ACCGATACCA CCGCTTCAAG GAACTCTCAA CCCTTAGGCG TCAGAAGCTG 
GAAGATTCCT ATCGATTCCA GTTCTTTCAA AGAGATGCTG AAGAGCTGGA GAAATGGATA 
CAGGAAAAAC TTCAGATTGC ATCTGATGAG AATTATAAAG ACCCAACCAA CTTGCAGGGA 
AAGCTTCAGA AGCATCAAGC ATTTGAAGCT GAAGTGCAGG CCAACTCAGG AGCCATTGTT 
AAGCTGGATG AAACTGGAAA CCTGATGATC TCAGAAGGGC ATTTTGCATC TGAAACCATA 
CGGACCCGTT TGATGGAGCT GCACCGCCAG TGGGAATTAC TTTTGGAGAA GATGCGAGAA 
AAAGGAATCA AACTGCTGCA GGCCCAGAAG TTGGTGCAGT ACTTACGAGA ATGTGAGGAC 
GTGATGGACT GGATCAATGA CAAGGAAGCA ATTGTTACTT CTGAAGAGCT GGGCCAGGAT 
CTGGAGCATG TAGAGGTTTT ACAGAAGAAA TTTGAAGAGT TTCAAACAGA TATGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTGAACCAG TTTGCTGCCA AACTCATACA GGAGCAGCAC 
CCTGAGGAGG AACTGATCAA GACTAAGCAG GATGAAGTCA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CTCTGCAGAG GCAGGGGAAG CTCTTTGGGG CAGCAGAAGT TCAGCGCTTT 
AACAGGGATG TGGATGAGAC TATCAGTTGG ATTAAGGAAA AGGAGCAGTT AATGGCCTCT 
GATGATTTTG GCCGAGACCT GGCAAGTGTT CAGGCTCTGC TTCGGAAGCA CGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAAGACAAG GTCAAAGCCC TGTGTGCTGA GGCTGACCGC 
CTGCAACAGT CCCACCCTCT GAGTGCAACA CAGATTCAAG TGAAGCGAGA GGAACTGATT 
ACAAACTGGG AGCAGATCCG CACCTTGGCG GCAGAGAGAC ATGCACGGCT CAATGATTCA 
TACAGGCTTC AACGCTTCCT TGCTGACTTC CGTGACCTCA CCAGCTGGGT GACTGAGATG 
AAAGCCCTCA TCAATGCAGA TGAGCTTGCC AGTGATGTGG CTGGGGCTGA AGCCCTGCTA 
GATAGACACC AAGAGCACAA GGGTGAAATT GATGCCCATG AAGACAGCTT CAAATCTGCA 
GATGAATCTG GACAGGCACT GCTTGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGACCG TCCTTTCCGA GGAGAGAGCG GCGCTGCTGG AGCTGTGGGA GCTGCGCAGG 
CAGCAGTACG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GGGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAATGAAG ACTTGGGAGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTTAA GAAGCACGAA GACTTTGAGA AATCCCTTAG TGCCCAGGAG 
GAAAAGATTA CAGCATTAGA TGAATTTGCA ACCAAGCTAA TTCAGAACAA CCACTATGCA 
ATGGAAGATG TGGCCACTCG CCGAGATGCT CTGTTGAGCC GCCGCAATGC CCTTCACGAG 
AGAGCCATGC GTCGCCGGGC CCAGCTAGCC GATTCTTTCC ATCTGCAGCA GTTTTTCCGT 
GATTCTGATG AGCTCAAGAG TTGGGTCAAT GAGAAGATGA AAACTGCCAC AGATGAAGCT 
TATAAAGATC CATCCAACCT ACAAGGAAAA GTACAGAAGC ATCAGGCTTT TGAGGCTGAG 
CTCTCAGCAA ACCAGAGCCG AATTGATGCC TTGGAGAAAG CTGGCCAAAA GCTGATTGAT 
GTCAACCACT ATGCCAAGGA TGAAGTGGCA GCTCGTATGA ATGAGGTGAT CAGTTTGTGG 
AAGAAACTGC TAGAGGCCAC TGAACTGAAA GGAATAAAGC TTCGTGAAGC CAACCAGCAA 
CAGCAATTTA ATCGCAATGT TGAGGATATT GAATTGTGGC TATATGAAGT AGAAGGTCAC 
TTGGCTTCGG ATGATTACGG CAAAGATCTT ACCAATGTGC AGAACCTCCA GAAGAAACAT 
GCACTGCTAG AGGCAGATGT GGCTGCTCAC CAGGACCGAA TTGATGGCAT CACCATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTT GATGCAGAAA ACATCAAGAA GAAACAGGAA 
GCCCTCGTGG CTCGCTATGA GGCACTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCCGATTCTC TGCGGTTGCA GCAGCTCTTC CGGGATGTTG AGGATGAGGA GACGTGGATT 
CGAGAGAAAG AGCCCATTGC CGCATCTACC AACAGAGGTA AGGATTTAAT TGGGGTCCAG 
AATCTGCTAA AGAAACATCA AGCCTTACAA GCAGAAATTG CTGGACATGA ACCACGCATC 
AAAGCAGTTA CACAGAAGGG GAATGCCATG GTGGAGGAAG GCCATTTTGC TGCAGAGGAT 
GTGAAGGCCA AGCTTCACGA GCTGAACCAA AAGTGGGAGG CACTGAAAGC CAAAGCTTCC 
CAGCGTCGGC AGGACCTGGA GGACTCTCTG CAGGCCCAGC AGTACTTTGC TGATGCTAAC 
GAGGCTGAAT CCTGGATGCG GGAGAAGGAA CCCATTGTGG GCAGCACTGA CTATGGCAAG 
GACGAAGACT CTGCTGAGGC TCTACTGAAG AAACACGAAG CTTTGATGTC AGATCTCAGT 
GCCTACGGCA GCAGCATCCA GGCTTTGCGA GAACAAGCAC AGTCCTGCCG GCAACAAGTG 
GCCCCCACGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CTCTCTACGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAG GGAGATATCC TTACCTTACT CAACAGCACC 
AACAAGGATT GGTGGAAAGT GGAAGTGAAC GATCGTCAGG GTTTTGTGCC GGCTGCGTAC 
GTGAAGAAAT TGGACCCCGC CCAGTCAGCC TCCCGGGAGA ATCTCCTGGA GGAGCAAGGC 
AGCATAGCAC TGCGGCAGGA GCAGATTGAC AATCAATATC ATTCTCTGCT GGAACTGGGT 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TTATGTTGTT CCGTGAAGCG 
AATGAACTAC AGCAATGGAT CAATGAGAAG GAAGCCGCTC TGACAAGTGA GGAGGTCGGA 
GCAGACTTGG AGCAGGTTGA GGTGCTCCAG AAGAAGTTTG ATGACTTCCA GAAGGACCTG 
AAGGCCAATG AGTCACGGTT GAAGGACATT AACAAGGTAG CTGAAGACCT GGAGTCTGAA 
GGTCTCATGG CAGAGGAGGT GCAGGCTGTG CAACAACAGG AAGTGTATGG CATGATGCCC 
AGGGATGAAA CTGATTCCAA GACAGCCTCC CCGTGGAAGT CTGCTCGTCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTACAGCAG 
CTGGCCGAGG AACGGAGCCA GCTCTTGGGC AGCGCCCATG AAGTACAGAG GTTCCACAGA 
GATGCTGATG AAACCAAAGA ATGGATTGAA GAGAAGAATC AAGCTCTAAA CACAGACAAT 
TATGGACATG ATCTCGCCAG TGTCCAGGCC CTGCAACGCA AGCATGAGGG CTTCGAGAGG 
GACCTTGCGG CTCTCGGTGA CAAGGTAAAC TCCCTTGGTG AAACAGCAGA GCGCCTGATC 
CAGTCCCATC CCGAGTCAGC AGAAGACCTG CAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGAGCAGCC TGGGGAAACG TGCAGATCAG CGCAAGGCAA AGTTGGGTGA CTCCCACGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGGGGG 
TTGGTGTCCT CAGATGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCATT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATCGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGACAGC AGCTGTTGGC TCACGGACAC TATGCCAGCC CTGAGATCAA GCAGAAACTT 
GATATTCTTG ACCAGGAGCG TGCAGACCTG GAGAAGGCCT GGGTTCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAACT GCAGCTGTTC CATCGGGACT GTGAGCAAGC TGAGAACTGG 
ATGGCTGCCC GGGAGGCCTT CTTGAATACC GAAGACAAAG GAGACTCACT GGACAGCGTA 
GAGGCTCTGA TCAAAAAACA TGAAGACTTT GACAAAGCGA TTAACGTCCA GGAAGAGAAG 
ATTGCTGCTC TGCAGGCCTT TGCCGACCAG CTCATCGCTG CCGGCCATTA TGCCAAGGGA 
GACATTTCTA GCCGGCGCAA TGAGGTCTTG GACAGGTGGC GACGTCTGAA AGCCCAGATG 
ATTGAGAAAA GGTCAAAGCT AGGAGAATCT CAAACCCTCC AACAGTTCAG CCGGGATGTG 
GATGAGATTG AGGCTTGGAT CAGTGAAAAA TTGCAAACAG CGAGTGATGA GTCGTACAAG 
GATCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ACCAGAAGCA CCAGGCTTTT 
GAAGCAGAGC TGCATGCCAA CGCTGACCGG ATCCGTGGGG TTATCGACAT GGGCAACTCC 
CTCATTGAAC GTGGAGCCTG TGCCGGCAGT GAGGATGCTG TCAAGGCCCG CCTGGCTGCC 
TTAGCTGACC AGTGGCAGTT CTTGGTGCAA AAGTCAGCGG AAAAGAGCCA GAAACTGAAA 
GAAGCCAACA AGCAGCAGAA CTTCAACACA GGGATCAAGG ACTTTGACTT CTGGCTGTCT 
GAGGTGGAGG CCCTGCTGGC ATCCGAAGAT TATGGCAAAG ACCTAGCTTC TGTGAACAAC 
CTGCTGAAAA AGCATCAACT GCTGGAAGCA GATATATCTG CCCATGAGGA TCGCCTGAAG 
GACCTGAACA GCCAGGCAGA CAGCCTGATG ACCAGCAGTG CCTTCGACAC CTCCCAAGTA 
AAGGACAAGA GGGACACCAT CAACGGGCGC TTCCAGAAGA TCAAGAGCAT GGCGGCCTCC 
CGGCGAGCCA AGCTGAATGA ATCCCATCGC CTGCACCAGT TCTTCCGGGA CATGGATGAC 
GAGGAGTCCT GGATCAAGGA GAAGAAGCTG CTGGTGGGCT CAGAGGACTA CGGCCGGGAC 
CTAACCGGCG TGCAGAACCT GAGGAAGAAG CACAAGCGGC TGGAAGCAGA ACTGGCTGCG 
CATGAGCCGG CTATTCAGGG TGTCCTGGAC ACTGGCAAGA AGCTGTCCGA TGACAACACC 
ATCGGGAAAG AGGAGATCCA GCAGCGGCTG GCGCAGTTTG TGGAGCACTG GAAAGAGCTG 
AAGCAGCTGG CAGCTGCCCG GGGTCAGCGG CTGGAAGAGT CCTTGGAATA TCAGCAGTTT 
GTAGCCAATG TGGAAGAGGA AGAAGCCTGG ATCAATGAGA AAATGACCCT GGTGGCCAGC 
GAAGATTATG GCGACACTCT TGCCGCCATC CAGGGCTTAC TGAAGAAACA TGAAGCTTTT 
GAGACAGACT TCACCGTCCA CAAGGATCGC GTGAATGATG TCTGCACCAA TGGACAAGAC 
CTCATTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CAAAGATGAA GGGCCTGAAC 
GGGAAAGTGT CAGACCTGGA GAAAGCTGCA GCCCAGAGAA AGGCGAAGCT GGATGAGAAC 
TCGGCCTTCC TTCAGTTCAA CTGGAAGGCG GACGTGGTGG AGTCCTGGAT CGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGC CGAGACCTGT CTTCTGTGCA GACGCTCCTC 
ACCAAACAGG AAACTTTTGA CGCTGGGCTG CAGGCCTTCC AGCAGGAAGG CATTGCCAAC 
ATCACTGCCC TCAAAGATCA GCTTCTCGCC GCCAAACACG TTCAGTCCAA GGCCATCGAG 
GCCCGGCACG CCTCCCTCAT GAAGAGGTGG AGCCAGCTTC TGGCCAACTC AGCCGCCCGC 
AAGAAGAAGC TTCTGGAGGC TCAGAGTCAC TTCCGCAAGG TGGAGGACCT CTTCCTGACC 
TTCGCCAAAA AGGCTTCTGC CTTCAACAGC TGGTTTGAAA ATGCAGAGGA GGACTTAACA 
GACCCCGTGC GCTGCAACTC CTTGGAAGAA ATCAAAGCTT TGCGCGAGGC CCACGACGCC 
TTCCGCTCCT CCCTCAGCTC TGCCCAGGCT GACTTCAACC AGCTGGCCGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT AGCCTCCAAC CCCTACACCT GGTTTACCAT GGAGGCCCTG 
GAGGAGACCT GGAGGAACCT ACAGAAAATC ATCAAGGAGA GGGAGCTGGA GCTGCAGAAG 
GAACAGCGGC GGCAGGAGGA GAACGACAAG CTGCGCCAGG AGTTTGCCCA GCACGCCAAC 
GCCTTCCACC AGTGGATCCA AGAGACCAGG ACATACCTCC TCGATGGGTC CTGTATGGTG 
GAAGAGTCGG GGACCCTCGA ATCCCAGCTT GAAGCTACCA AACGCAAGCA CCAGGAAATC 
CGAGCCATGA GAAGTCAGCT CAAAAAGATC GAGGACCTGG GGGCCGCCAT GGAGGAGGCC 
CTCATCCTGG ACAACAAGTA CACGGAGCAC AGCACCGTGG GCCTCGCCCA GCAGTGGGAC 
CAGCTGGACC AGCTGGGCAT GCGCATGCAG CACAACCTGG AGCAGCAGAT CCAGGCCAGG 
AACACAACAG GTGTGACTGA GGAGGCCCTC AAAGAATTCA GCATGATGTT TAAACACTTT 
GACAAGGACA AGTCTGGCAG GCTGAACCAT CAGGAGTTCA AATCTTGCCT GCGCTCCCTG 
GGCTATGACC TGCCCATGGT GGAGGAAGGG GAACCTGACC CTGAGTTCGA GGCAATCCTG 
GACACGGTGG ATCCGAACAG AGATGGCCAT GTCTCCTTGC AAGAATACAT GGCTTTCATG 
ATCAGCCGCG AAACTGAGAA CGTCAAGTCC AGCGAGGAGA TTGAGAGCGC CTTCCGGGCC 
CTCAGCTCAG AGGGAAAGCC TTACGTGACC AAGGAGGAGC TCTACCAGAA CCTGACCCGG 
GAACAAGCCG ACTACTGCGT CTCCCACATG AAGCCCTACG TGGACGGCAA GGGCCGCGAG 
CTCCCCACCG CGTTCGACTA CGTGGAGTTC ACCCGCTCGC TTTTCGTGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( NM_001195532.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001182461.1 Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant 3, mRNA.

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CloneID	OHu10360
Clone ID Related Accession (Same CDS sequence)	NM_001195532.1
Accession Version	NM_001195532.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Homo sapiens(human)
Product	spectrin alpha chain, non-erythrocytic 1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC359079.1, AB191262.1, BC053521.1 and AL356481.16. Transcript Variant: This variant (3) lacks two in-frame exons in the coding region, compared to variant 1. The encoded isoform (3) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB191262.1, BC053521.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACCCAA GTGGGGTCAA AGTGCTGGAA ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTAG ACCGATACCA CCGCTTCAAG GAACTCTCAA CCCTTAGGCG TCAGAAGCTG 
GAAGATTCCT ATCGATTCCA GTTCTTTCAA AGAGATGCTG AAGAGCTGGA GAAATGGATA 
CAGGAAAAAC TTCAGATTGC ATCTGATGAG AATTATAAAG ACCCAACCAA CTTGCAGGGA 
AAGCTTCAGA AGCATCAAGC ATTTGAAGCT GAAGTGCAGG CCAACTCAGG AGCCATTGTT 
AAGCTGGATG AAACTGGAAA CCTGATGATC TCAGAAGGGC ATTTTGCATC TGAAACCATA 
CGGACCCGTT TGATGGAGCT GCACCGCCAG TGGGAATTAC TTTTGGAGAA GATGCGAGAA 
AAAGGAATCA AACTGCTGCA GGCCCAGAAG TTGGTGCAGT ACTTACGAGA ATGTGAGGAC 
GTGATGGACT GGATCAATGA CAAGGAAGCA ATTGTTACTT CTGAAGAGCT GGGCCAGGAT 
CTGGAGCATG TAGAGGTTTT ACAGAAGAAA TTTGAAGAGT TTCAAACAGA TATGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTGAACCAG TTTGCTGCCA AACTCATACA GGAGCAGCAC 
CCTGAGGAGG AACTGATCAA GACTAAGCAG GATGAAGTCA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CTCTGCAGAG GCAGGGGAAG CTCTTTGGGG CAGCAGAAGT TCAGCGCTTT 
AACAGGGATG TGGATGAGAC TATCAGTTGG ATTAAGGAAA AGGAGCAGTT AATGGCCTCT 
GATGATTTTG GCCGAGACCT GGCAAGTGTT CAGGCTCTGC TTCGGAAGCA CGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAAGACAAG GTCAAAGCCC TGTGTGCTGA GGCTGACCGC 
CTGCAACAGT CCCACCCTCT GAGTGCAACA CAGATTCAAG TGAAGCGAGA GGAACTGATT 
ACAAACTGGG AGCAGATCCG CACCTTGGCG GCAGAGAGAC ATGCACGGCT CAATGATTCA 
TACAGGCTTC AACGCTTCCT TGCTGACTTC CGTGACCTCA CCAGCTGGGT GACTGAGATG 
AAAGCCCTCA TCAATGCAGA TGAGCTTGCC AGTGATGTGG CTGGGGCTGA AGCCCTGCTA 
GATAGACACC AAGAGCACAA GGGTGAAATT GATGCCCATG AAGACAGCTT CAAATCTGCA 
GATGAATCTG GACAGGCACT GCTTGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGACCG TCCTTTCCGA GGAGAGAGCG GCGCTGCTGG AGCTGTGGGA GCTGCGCAGG 
CAGCAGTACG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GGGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAATGAAG ACTTGGGAGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTTAA GAAGCACGAA GACTTTGAGA AATCCCTTAG TGCCCAGGAG 
GAAAAGATTA CAGCATTAGA TGAATTTGCA ACCAAGCTAA TTCAGAACAA CCACTATGCA 
ATGGAAGATG TGGCCACTCG CCGAGATGCT CTGTTGAGCC GCCGCAATGC CCTTCACGAG 
AGAGCCATGC GTCGCCGGGC CCAGCTAGCC GATTCTTTCC ATCTGCAGCA GTTTTTCCGT 
GATTCTGATG AGCTCAAGAG TTGGGTCAAT GAGAAGATGA AAACTGCCAC AGATGAAGCT 
TATAAAGATC CATCCAACCT ACAAGGAAAA GTACAGAAGC ATCAGGCTTT TGAGGCTGAG 
CTCTCAGCAA ACCAGAGCCG AATTGATGCC TTGGAGAAAG CTGGCCAAAA GCTGATTGAT 
GTCAACCACT ATGCCAAGGA TGAAGTGGCA GCTCGTATGA ATGAGGTGAT CAGTTTGTGG 
AAGAAACTGC TAGAGGCCAC TGAACTGAAA GGAATAAAGC TTCGTGAAGC CAACCAGCAA 
CAGCAATTTA ATCGCAATGT TGAGGATATT GAATTGTGGC TATATGAAGT AGAAGGTCAC 
TTGGCTTCGG ATGATTACGG CAAAGATCTT ACCAATGTGC AGAACCTCCA GAAGAAACAT 
GCCCTGCTAG AGGCAGATGT GGCTGCTCAC CAGGACCGAA TTGATGGCAT CACCATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTT GATGCAGAAA ACATCAAGAA GAAACAGGAA 
GCCCTCGTGG CTCGCTATGA GGCACTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCCGATTCTC TGCGGTTGCA GCAGCTCTTC CGGGATGTTG AGGATGAGGA GACGTGGATT 
CGAGAGAAAG AGCCCATTGC CGCATCTACC AACAGAGGTA AGGATTTAAT TGGGGTCCAG 
AATCTGCTAA AGAAACATCA AGCCTTACAA GCAGAAATTG CTGGACATGA ACCACGCATC 
AAAGCAGTTA CACAGAAGGG GAATGCCATG GTGGAGGAAG GCCATTTTGC TGCAGAGGAT 
GTGAAGGCCA AGCTTCACGA GCTGAACCAA AAGTGGGAGG CACTGAAAGC CAAAGCTTCC 
CAGCGTCGGC AGGACCTGGA GGACTCTCTG CAGGCCCAGC AGTACTTTGC TGATGCTAAC 
GAGGCTGAAT CCTGGATGCG GGAGAAGGAA CCCATTGTGG GCAGCACTGA CTATGGCAAG 
GACGAAGACT CTGCTGAGGC TCTACTGAAG AAACACGAAG CTTTGATGTC AGATCTCAGT 
GCCTACGGCA GCAGCATCCA GGCTTTGCGA GAACAAGCAC AGTCCTGCCG GCAACAAGTG 
GCCCCCACGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CTCTCTACGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAG GGAGATATCC TTACCTTACT CAACAGCACC 
AACAAGGATT GGTGGAAAGT GGAAGTGAAC GATCGTCAGG GTTTTGTGCC GGCTGCGTAC 
GTGAAGAAAT TGGACCCCGC CCAGTCAGCC TCCCGGGAGA ATCTCCTGGA GGAGCAAGGC 
AGCATAGCAC TGCGGCAGGA GCAGATTGAC AATCAATATC ATTCTCTGCT GGAACTGGGT 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TTATGTTGTT CCGTGAAGCG 
AATGAACTAC AGCAATGGAT CAATGAGAAG GAAGCCGCTC TGACAAGTGA GGAGGTCGGA 
GCAGACTTGG AGCAGGTTGA GGTGCTCCAG AAGAAGTTTG ATGACTTCCA GAAGGACCTG 
AAGGCCAATG AGTCACGGTT GAAGGACATT AACAAGGTAG CTGAAGACCT GGAGTCTGAA 
GGTCTCATGG CAGAGGAGGT GCAGGCTGTG CAACAACAGG AAGTGTATGG CATGATGCCC 
AGGGATGAAA CTGATTCCAA GACAGCCTCC CCGTGGAAGT CTGCTCGTCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTACAGCAG 
CTGGCCGAGG AACGGAGCCA GCTCTTGGGC AGCGCCCATG AAGTACAGAG GTTCCACAGA 
GATGCTGATG AAACCAAAGA ATGGATTGAA GAGAAGAATC AAGCTCTAAA CACAGACAAT 
TATGGACATG ATCTCGCCAG TGTCCAGGCC CTGCAACGCA AGCATGAGGG CTTCGAGAGG 
GACCTTGCGG CTCTCGGTGA CAAGGTAAAC TCCCTTGGTG AAACAGCAGA GCGCCTGATC 
CAGTCCCATC CCGAGTCAGC AGAAGACCTG CAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGAGCAGCC TGGGGAAACG TGCAGATCAG CGCAAGGCAA AGTTGGGTGA CTCCCACGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGGGGG 
TTGGTGTCCT CAGATGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCATT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATCGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGACAGC AGCTGTTGGC TCACGGACAC TATGCCAGCC CTGAGATCAA GCAGAAACTT 
GATATTCTTG ACCAGGAGCG TGCAGACCTG GAGAAGGCCT GGGTTCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAACT GCAGCTGTTC CATCGGGACT GTGAGCAAGC TGAGAACTGG 
ATGGCTGCCC GGGAGGCCTT CTTGAATACC GAAGACAAAG GAGACTCACT GGACAGCGTA 
GAGGCTCTGA TCAAAAAACA TGAAGACTTT GACAAAGCGA TTAACGTCCA GGAAGAGAAG 
ATTGCTGCTC TGCAGGCCTT TGCCGACCAG CTCATCGCTG CCGGCCATTA TGCCAAGGGA 
GACATTTCTA GCCGGCGCAA TGAGGTCTTG GACAGGTGGC GACGTCTGAA AGCCCAGATG 
ATTGAGAAAA GGTCAAAGCT AGGAGAATCT CAAACCCTCC AACAGTTCAG CCGGGATGTG 
GATGAGATTG AGGCTTGGAT CAGTGAAAAA TTGCAAACAG CGAGTGATGA GTCGTACAAG 
GATCCCACCA ACATCCAGAG CAAGCACCAG AAGCACCAGG CTTTTGAAGC AGAGCTGCAT 
GCCAACGCTG ACCGGATCCG TGGGGTTATC GACATGGGCA ACTCCCTCAT TGAACGTGGA 
GCCTGTGCCG GCAGTGAGGA TGCTGTCAAG GCCCGCCTGG CTGCCTTAGC TGACCAGTGG 
CAGTTCTTGG TGCAAAAGTC AGCGGAAAAG AGCCAGAAAC TGAAAGAAGC CAACAAGCAG 
CAGAACTTCA ACACAGGGAT CAAGGACTTT GACTTCTGGC TGTCTGAGGT GGAGGCCCTG 
CTGGCATCCG AAGATTATGG CAAAGACCTG GCTTCTGTGA ACAACCTGCT GAAAAAGCAT 
CAACTGCTGG AAGCAGATAT ATCTGCCCAT GAGGATCGCC TGAAGGACCT GAACAGCCAG 
GCAGACAGCC TGATGACCAG CAGTGCCTTC GACACCTCCC AAGTAAAGGA CAAGAGGGAC 
ACCATCAACG GGCGCTTCCA GAAGATCAAG AGCATGGCGG CCTCCCGGCG AGCCAAGCTG 
AATGAATCCC ATCGCCTGCA CCAGTTCTTC CGGGACATGG ATGACGAGGA GTCCTGGATC 
AAGGAGAAGA AGCTGCTGGT GGGCTCAGAG GACTACGGCC GGGACCTAAC CGGCGTGCAG 
AACCTGAGGA AGAAGCACAA GCGGCTGGAA GCAGAACTGG CTGCGCATGA GCCGGCTATT 
CAGGGTGTCC TGGACACTGG CAAGAAGCTG TCCGATGACA ACACCATCGG GAAAGAGGAG 
ATCCAGCAGC GGCTGGCGCA GTTTGTGGAG CACTGGAAAG AGCTGAAGCA GCTGGCAGCT 
GCCCGGGGTC AGCGGCTGGA AGAGTCCTTG GAATATCAGC AGTTTGTAGC CAATGTGGAA 
GAGGAAGAAG CCTGGATCAA TGAGAAAATG ACCCTGGTGG CCAGCGAAGA TTATGGCGAC 
ACTCTTGCCG CCATCCAGGG CTTACTGAAG AAACATGAAG CTTTTGAGAC AGACTTCACC 
GTCCACAAGG ATCGCGTGAA TGATGTCTGC ACCAATGGAC AAGACCTCAT TAAGAAGAAC 
AATCACCATG AGGAGAACAT CTCTTCAAAG ATGAAGGGCC TGAACGGGAA AGTGTCAGAC 
CTGGAGAAAG CTGCAGCCCA GAGAAAGGCG AAGCTGGATG AGAACTCGGC CTTCCTTCAG 
TTCAACTGGA AGGCGGACGT GGTGGAGTCC TGGATCGGTG AAAAGGAGAA CAGCTTGAAG 
ACAGATGATT ATGGCCGAGA CCTGTCTTCT GTGCAGACGC TCCTCACCAA ACAGGAAACT 
TTTGACGCTG GGCTGCAGGC CTTCCAGCAG GAAGGCATTG CCAACATCAC TGCCCTCAAA 
GATCAGCTTC TCGCCGCCAA ACACGTTCAG TCCAAGGCCA TCGAGGCCCG GCACGCCTCC 
CTCATGAAGA GGTGGAGCCA GCTTCTGGCC AACTCAGCCG CCCGCAAGAA GAAGCTTCTG 
GAGGCTCAGA GTCACTTCCG CAAGGTGGAG GACCTCTTCC TGACCTTCGC CAAAAAGGCT 
TCTGCCTTCA ACAGCTGGTT TGAAAATGCA GAGGAGGACT TAACAGACCC CGTGCGCTGC 
AACTCCTTGG AAGAAATCAA AGCTTTGCGC GAGGCCCACG ACGCCTTCCG CTCCTCCCTC 
AGCTCTGCCC AGGCTGACTT CAACCAGCTG GCCGAGCTGG ACCGCCAGAT CAAGAGCTTC 
CGCGTAGCCT CCAACCCCTA CACCTGGTTT ACCATGGAGG CCCTGGAGGA GACCTGGAGG 
AACCTACAGA AAATCATCAA GGAGAGGGAG CTGGAGCTGC AGAAGGAACA GCGGCGGCAG 
GAGGAGAACG ACAAGCTGCG CCAGGAGTTT GCCCAGCACG CCAACGCCTT CCACCAGTGG 
ATCCAAGAGA CCAGGACATA CCTCCTCGAT GGGTCCTGTA TGGTGGAAGA GTCGGGGACC 
CTCGAATCCC AGCTTGAAGC TACCAAACGC AAGCACCAGG AAATCCGAGC CATGAGAAGT 
CAGCTCAAAA AGATCGAGGA CCTGGGGGCC GCCATGGAGG AGGCCCTCAT CCTGGACAAC 
AAGTACACGG AGCACAGCAC CGTGGGCCTC GCCCAGCAGT GGGACCAGCT GGACCAGCTG 
GGCATGCGCA TGCAGCACAA CCTGGAGCAG CAGATCCAGG CCAGGAACAC AACAGGTGTG 
ACTGAGGAGG CCCTCAAAGA ATTCAGCATG ATGTTTAAAC ACTTTGACAA GGACAAGTCT 
GGCAGGCTGA ACCATCAGGA GTTCAAATCT TGCCTGCGCT CCCTGGGCTA TGACCTGCCC 
ATGGTGGAGG AAGGGGAACC TGACCCTGAG TTCGAGGCAA TCCTGGACAC GGTGGATCCG 
AACAGAGATG GCCATGTCTC CTTGCAAGAA TACATGGCTT TCATGATCAG CCGCGAAACT 
GAGAACGTCA AGTCCAGCGA GGAGATTGAG AGCGCCTTCC GGGCCCTCAG CTCAGAGGGA 
AAGCCTTACG TGACCAAGGA GGAGCTCTAC CAGAACCTGA CCCGGGAACA AGCCGACTAC 
TGCGTCTCCC ACATGAAGCC CTACGTGGAC GGCAAGGGCC GCGAGCTCCC CACCGCGTTC 
GACTACGTGG AGTTCACCCG CTCGCTTTTC GTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001182461.1
CDS	143..7501
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELASDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMRRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPTDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGMMPRDETDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKQKLDILDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAAGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINGRFQKIKSMAASRRAKLNESHRLHQFFRDMDDEESWIKEKKLLVGSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHASLMKRWSQLLANSAARKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	NM_001195532.1
Organism	Homo sapiens(human)
Definition	Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001195532.1

ORF Insert Sequence:

ATGGACCCAA GTGGGGTCAA AGTGCTGGAA ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTAG ACCGATACCA CCGCTTCAAG GAACTCTCAA CCCTTAGGCG TCAGAAGCTG 
GAAGATTCCT ATCGATTCCA GTTCTTTCAA AGAGATGCTG AAGAGCTGGA GAAATGGATA 
CAGGAAAAAC TTCAGATTGC ATCTGATGAG AATTATAAAG ACCCAACCAA CTTGCAGGGA 
AAGCTTCAGA AGCATCAAGC ATTTGAAGCT GAAGTGCAGG CCAACTCAGG AGCCATTGTT 
AAGCTGGATG AAACTGGAAA CCTGATGATC TCAGAAGGGC ATTTTGCATC TGAAACCATA 
CGGACCCGTT TGATGGAGCT GCACCGCCAG TGGGAATTAC TTTTGGAGAA GATGCGAGAA 
AAAGGAATCA AACTGCTGCA GGCCCAGAAG TTGGTGCAGT ACTTACGAGA ATGTGAGGAC 
GTGATGGACT GGATCAATGA CAAGGAAGCA ATTGTTACTT CTGAAGAGCT GGGCCAGGAT 
CTGGAGCATG TAGAGGTTTT ACAGAAGAAA TTTGAAGAGT TTCAAACAGA TATGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTGAACCAG TTTGCTGCCA AACTCATACA GGAGCAGCAC 
CCTGAGGAGG AACTGATCAA GACTAAGCAG GATGAAGTCA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CTCTGCAGAG GCAGGGGAAG CTCTTTGGGG CAGCAGAAGT TCAGCGCTTT 
AACAGGGATG TGGATGAGAC TATCAGTTGG ATTAAGGAAA AGGAGCAGTT AATGGCCTCT 
GATGATTTTG GCCGAGACCT GGCAAGTGTT CAGGCTCTGC TTCGGAAGCA CGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAAGACAAG GTCAAAGCCC TGTGTGCTGA GGCTGACCGC 
CTGCAACAGT CCCACCCTCT GAGTGCAACA CAGATTCAAG TGAAGCGAGA GGAACTGATT 
ACAAACTGGG AGCAGATCCG CACCTTGGCG GCAGAGAGAC ATGCACGGCT CAATGATTCA 
TACAGGCTTC AACGCTTCCT TGCTGACTTC CGTGACCTCA CCAGCTGGGT GACTGAGATG 
AAAGCCCTCA TCAATGCAGA TGAGCTTGCC AGTGATGTGG CTGGGGCTGA AGCCCTGCTA 
GATAGACACC AAGAGCACAA GGGTGAAATT GATGCCCATG AAGACAGCTT CAAATCTGCA 
GATGAATCTG GACAGGCACT GCTTGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGACCG TCCTTTCCGA GGAGAGAGCG GCGCTGCTGG AGCTGTGGGA GCTGCGCAGG 
CAGCAGTACG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GGGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAATGAAG ACTTGGGAGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTTAA GAAGCACGAA GACTTTGAGA AATCCCTTAG TGCCCAGGAG 
GAAAAGATTA CAGCATTAGA TGAATTTGCA ACCAAGCTAA TTCAGAACAA CCACTATGCA 
ATGGAAGATG TGGCCACTCG CCGAGATGCT CTGTTGAGCC GCCGCAATGC CCTTCACGAG 
AGAGCCATGC GTCGCCGGGC CCAGCTAGCC GATTCTTTCC ATCTGCAGCA GTTTTTCCGT 
GATTCTGATG AGCTCAAGAG TTGGGTCAAT GAGAAGATGA AAACTGCCAC AGATGAAGCT 
TATAAAGATC CATCCAACCT ACAAGGAAAA GTACAGAAGC ATCAGGCTTT TGAGGCTGAG 
CTCTCAGCAA ACCAGAGCCG AATTGATGCC TTGGAGAAAG CTGGCCAAAA GCTGATTGAT 
GTCAACCACT ATGCCAAGGA TGAAGTGGCA GCTCGTATGA ATGAGGTGAT CAGTTTGTGG 
AAGAAACTGC TAGAGGCCAC TGAACTGAAA GGAATAAAGC TTCGTGAAGC CAACCAGCAA 
CAGCAATTTA ATCGCAATGT TGAGGATATT GAATTGTGGC TATATGAAGT AGAAGGTCAC 
TTGGCTTCGG ATGATTACGG CAAAGATCTT ACCAATGTGC AGAACCTCCA GAAGAAACAT 
GCCCTGCTAG AGGCAGATGT GGCTGCTCAC CAGGACCGAA TTGATGGCAT CACCATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTT GATGCAGAAA ACATCAAGAA GAAACAGGAA 
GCCCTCGTGG CTCGCTATGA GGCACTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCCGATTCTC TGCGGTTGCA GCAGCTCTTC CGGGATGTTG AGGATGAGGA GACGTGGATT 
CGAGAGAAAG AGCCCATTGC CGCATCTACC AACAGAGGTA AGGATTTAAT TGGGGTCCAG 
AATCTGCTAA AGAAACATCA AGCCTTACAA GCAGAAATTG CTGGACATGA ACCACGCATC 
AAAGCAGTTA CACAGAAGGG GAATGCCATG GTGGAGGAAG GCCATTTTGC TGCAGAGGAT 
GTGAAGGCCA AGCTTCACGA GCTGAACCAA AAGTGGGAGG CACTGAAAGC CAAAGCTTCC 
CAGCGTCGGC AGGACCTGGA GGACTCTCTG CAGGCCCAGC AGTACTTTGC TGATGCTAAC 
GAGGCTGAAT CCTGGATGCG GGAGAAGGAA CCCATTGTGG GCAGCACTGA CTATGGCAAG 
GACGAAGACT CTGCTGAGGC TCTACTGAAG AAACACGAAG CTTTGATGTC AGATCTCAGT 
GCCTACGGCA GCAGCATCCA GGCTTTGCGA GAACAAGCAC AGTCCTGCCG GCAACAAGTG 
GCCCCCACGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CTCTCTACGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAG GGAGATATCC TTACCTTACT CAACAGCACC 
AACAAGGATT GGTGGAAAGT GGAAGTGAAC GATCGTCAGG GTTTTGTGCC GGCTGCGTAC 
GTGAAGAAAT TGGACCCCGC CCAGTCAGCC TCCCGGGAGA ATCTCCTGGA GGAGCAAGGC 
AGCATAGCAC TGCGGCAGGA GCAGATTGAC AATCAATATC ATTCTCTGCT GGAACTGGGT 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TTATGTTGTT CCGTGAAGCG 
AATGAACTAC AGCAATGGAT CAATGAGAAG GAAGCCGCTC TGACAAGTGA GGAGGTCGGA 
GCAGACTTGG AGCAGGTTGA GGTGCTCCAG AAGAAGTTTG ATGACTTCCA GAAGGACCTG 
AAGGCCAATG AGTCACGGTT GAAGGACATT AACAAGGTAG CTGAAGACCT GGAGTCTGAA 
GGTCTCATGG CAGAGGAGGT GCAGGCTGTG CAACAACAGG AAGTGTATGG CATGATGCCC 
AGGGATGAAA CTGATTCCAA GACAGCCTCC CCGTGGAAGT CTGCTCGTCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTACAGCAG 
CTGGCCGAGG AACGGAGCCA GCTCTTGGGC AGCGCCCATG AAGTACAGAG GTTCCACAGA 
GATGCTGATG AAACCAAAGA ATGGATTGAA GAGAAGAATC AAGCTCTAAA CACAGACAAT 
TATGGACATG ATCTCGCCAG TGTCCAGGCC CTGCAACGCA AGCATGAGGG CTTCGAGAGG 
GACCTTGCGG CTCTCGGTGA CAAGGTAAAC TCCCTTGGTG AAACAGCAGA GCGCCTGATC 
CAGTCCCATC CCGAGTCAGC AGAAGACCTG CAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGAGCAGCC TGGGGAAACG TGCAGATCAG CGCAAGGCAA AGTTGGGTGA CTCCCACGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGGGGG 
TTGGTGTCCT CAGATGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCATT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATCGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGACAGC AGCTGTTGGC TCACGGACAC TATGCCAGCC CTGAGATCAA GCAGAAACTT 
GATATTCTTG ACCAGGAGCG TGCAGACCTG GAGAAGGCCT GGGTTCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAACT GCAGCTGTTC CATCGGGACT GTGAGCAAGC TGAGAACTGG 
ATGGCTGCCC GGGAGGCCTT CTTGAATACC GAAGACAAAG GAGACTCACT GGACAGCGTA 
GAGGCTCTGA TCAAAAAACA TGAAGACTTT GACAAAGCGA TTAACGTCCA GGAAGAGAAG 
ATTGCTGCTC TGCAGGCCTT TGCCGACCAG CTCATCGCTG CCGGCCATTA TGCCAAGGGA 
GACATTTCTA GCCGGCGCAA TGAGGTCTTG GACAGGTGGC GACGTCTGAA AGCCCAGATG 
ATTGAGAAAA GGTCAAAGCT AGGAGAATCT CAAACCCTCC AACAGTTCAG CCGGGATGTG 
GATGAGATTG AGGCTTGGAT CAGTGAAAAA TTGCAAACAG CGAGTGATGA GTCGTACAAG 
GATCCCACCA ACATCCAGAG CAAGCACCAG AAGCACCAGG CTTTTGAAGC AGAGCTGCAT 
GCCAACGCTG ACCGGATCCG TGGGGTTATC GACATGGGCA ACTCCCTCAT TGAACGTGGA 
GCCTGTGCCG GCAGTGAGGA TGCTGTCAAG GCCCGCCTGG CTGCCTTAGC TGACCAGTGG 
CAGTTCTTGG TGCAAAAGTC AGCGGAAAAG AGCCAGAAAC TGAAAGAAGC CAACAAGCAG 
CAGAACTTCA ACACAGGGAT CAAGGACTTT GACTTCTGGC TGTCTGAGGT GGAGGCCCTG 
CTGGCATCCG AAGATTATGG CAAAGACCTG GCTTCTGTGA ACAACCTGCT GAAAAAGCAT 
CAACTGCTGG AAGCAGATAT ATCTGCCCAT GAGGATCGCC TGAAGGACCT GAACAGCCAG 
GCAGACAGCC TGATGACCAG CAGTGCCTTC GACACCTCCC AAGTAAAGGA CAAGAGGGAC 
ACCATCAACG GGCGCTTCCA GAAGATCAAG AGCATGGCGG CCTCCCGGCG AGCCAAGCTG 
AATGAATCCC ATCGCCTGCA CCAGTTCTTC CGGGACATGG ATGACGAGGA GTCCTGGATC 
AAGGAGAAGA AGCTGCTGGT GGGCTCAGAG GACTACGGCC GGGACCTAAC CGGCGTGCAG 
AACCTGAGGA AGAAGCACAA GCGGCTGGAA GCAGAACTGG CTGCGCATGA GCCGGCTATT 
CAGGGTGTCC TGGACACTGG CAAGAAGCTG TCCGATGACA ACACCATCGG GAAAGAGGAG 
ATCCAGCAGC GGCTGGCGCA GTTTGTGGAG CACTGGAAAG AGCTGAAGCA GCTGGCAGCT 
GCCCGGGGTC AGCGGCTGGA AGAGTCCTTG GAATATCAGC AGTTTGTAGC CAATGTGGAA 
GAGGAAGAAG CCTGGATCAA TGAGAAAATG ACCCTGGTGG CCAGCGAAGA TTATGGCGAC 
ACTCTTGCCG CCATCCAGGG CTTACTGAAG AAACATGAAG CTTTTGAGAC AGACTTCACC 
GTCCACAAGG ATCGCGTGAA TGATGTCTGC ACCAATGGAC AAGACCTCAT TAAGAAGAAC 
AATCACCATG AGGAGAACAT CTCTTCAAAG ATGAAGGGCC TGAACGGGAA AGTGTCAGAC 
CTGGAGAAAG CTGCAGCCCA GAGAAAGGCG AAGCTGGATG AGAACTCGGC CTTCCTTCAG 
TTCAACTGGA AGGCGGACGT GGTGGAGTCC TGGATCGGTG AAAAGGAGAA CAGCTTGAAG 
ACAGATGATT ATGGCCGAGA CCTGTCTTCT GTGCAGACGC TCCTCACCAA ACAGGAAACT 
TTTGACGCTG GGCTGCAGGC CTTCCAGCAG GAAGGCATTG CCAACATCAC TGCCCTCAAA 
GATCAGCTTC TCGCCGCCAA ACACGTTCAG TCCAAGGCCA TCGAGGCCCG GCACGCCTCC 
CTCATGAAGA GGTGGAGCCA GCTTCTGGCC AACTCAGCCG CCCGCAAGAA GAAGCTTCTG 
GAGGCTCAGA GTCACTTCCG CAAGGTGGAG GACCTCTTCC TGACCTTCGC CAAAAAGGCT 
TCTGCCTTCA ACAGCTGGTT TGAAAATGCA GAGGAGGACT TAACAGACCC CGTGCGCTGC 
AACTCCTTGG AAGAAATCAA AGCTTTGCGC GAGGCCCACG ACGCCTTCCG CTCCTCCCTC 
AGCTCTGCCC AGGCTGACTT CAACCAGCTG GCCGAGCTGG ACCGCCAGAT CAAGAGCTTC 
CGCGTAGCCT CCAACCCCTA CACCTGGTTT ACCATGGAGG CCCTGGAGGA GACCTGGAGG 
AACCTACAGA AAATCATCAA GGAGAGGGAG CTGGAGCTGC AGAAGGAACA GCGGCGGCAG 
GAGGAGAACG ACAAGCTGCG CCAGGAGTTT GCCCAGCACG CCAACGCCTT CCACCAGTGG 
ATCCAAGAGA CCAGGACATA CCTCCTCGAT GGGTCCTGTA TGGTGGAAGA GTCGGGGACC 
CTCGAATCCC AGCTTGAAGC TACCAAACGC AAGCACCAGG AAATCCGAGC CATGAGAAGT 
CAGCTCAAAA AGATCGAGGA CCTGGGGGCC GCCATGGAGG AGGCCCTCAT CCTGGACAAC 
AAGTACACGG AGCACAGCAC CGTGGGCCTC GCCCAGCAGT GGGACCAGCT GGACCAGCTG 
GGCATGCGCA TGCAGCACAA CCTGGAGCAG CAGATCCAGG CCAGGAACAC AACAGGTGTG 
ACTGAGGAGG CCCTCAAAGA ATTCAGCATG ATGTTTAAAC ACTTTGACAA GGACAAGTCT 
GGCAGGCTGA ACCATCAGGA GTTCAAATCT TGCCTGCGCT CCCTGGGCTA TGACCTGCCC 
ATGGTGGAGG AAGGGGAACC TGACCCTGAG TTCGAGGCAA TCCTGGACAC GGTGGATCCG 
AACAGAGATG GCCATGTCTC CTTGCAAGAA TACATGGCTT TCATGATCAG CCGCGAAACT 
GAGAACGTCA AGTCCAGCGA GGAGATTGAG AGCGCCTTCC GGGCCCTCAG CTCAGAGGGA 
AAGCCTTACG TGACCAAGGA GGAGCTCTAC CAGAACCTGA CCCGGGAACA AGCCGACTAC 
TGCGTCTCCC ACATGAAGCC CTACGTGGAC GGCAAGGGCC GCGAGCTCCC CACCGCGTTC 
GACTACGTGG AGTTCACCCG CTCGCTTTTC GTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( NM_003127.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_003118.2 Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant 2, mRNA.

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CloneID	OHu12626
Clone ID Related Accession (Same CDS sequence)	NM_003127.3
Accession Version	NM_003127.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7419bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	spectrin alpha chain, non-erythrocytic 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC359079.1, AB209860.1, AL356481.16 and BC053521.1. On Sep 15, 2010 this sequence version replaced NM_003127.2. Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, compared to variant 1. The resulting isoform (2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB209860.1, J05243.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACCCAA GTGGGGTCAA AGTGCTGGAA ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTAG ACCGATACCA CCGCTTCAAG GAACTCTCAA CCCTTAGGCG TCAGAAGCTG 
GAAGATTCCT ATCGATTCCA GTTCTTTCAA AGAGATGCTG AAGAGCTGGA GAAATGGATA 
CAGGAAAAAC TTCAGATTGC ATCTGATGAG AATTATAAAG ACCCAACCAA CTTGCAGGGA 
AAGCTTCAGA AGCATCAAGC ATTTGAAGCT GAAGTGCAGG CCAACTCAGG AGCCATTGTT 
AAGCTGGATG AAACTGGAAA CCTGATGATC TCAGAAGGGC ATTTTGCATC TGAAACCATA 
CGGACCCGTT TGATGGAGCT GCACCGCCAG TGGGAATTAC TTTTGGAGAA GATGCGAGAA 
AAAGGAATCA AACTGCTGCA GGCCCAGAAG TTGGTGCAGT ACTTACGAGA ATGTGAGGAC 
GTGATGGACT GGATCAATGA CAAGGAAGCA ATTGTTACTT CTGAAGAGCT GGGCCAGGAT 
CTGGAGCATG TAGAGGTTTT ACAGAAGAAA TTTGAAGAGT TTCAAACAGA TATGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTGAACCAG TTTGCTGCCA AACTCATACA GGAGCAGCAC 
CCTGAGGAGG AACTGATCAA GACTAAGCAG GATGAAGTCA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CTCTGCAGAG GCAGGGGAAG CTCTTTGGGG CAGCAGAAGT TCAGCGCTTT 
AACAGGGATG TGGATGAGAC TATCAGTTGG ATTAAGGAAA AGGAGCAGTT AATGGCCTCT 
GATGATTTTG GCCGAGACCT GGCAAGTGTT CAGGCTCTGC TTCGGAAGCA CGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAAGACAAG GTCAAAGCCC TGTGTGCTGA GGCTGACCGC 
CTGCAACAGT CCCACCCTCT GAGTGCAACA CAGATTCAAG TGAAGCGAGA GGAACTGATT 
ACAAACTGGG AGCAGATCCG CACCTTGGCG GCAGAGAGAC ATGCACGGCT CAATGATTCA 
TACAGGCTTC AACGCTTCCT TGCTGACTTC CGTGACCTCA CCAGCTGGGT GACTGAGATG 
AAAGCCCTCA TCAATGCAGA TGAGCTTGCC AGTGATGTGG CTGGGGCTGA AGCCCTGCTA 
GATAGACACC AAGAGCACAA GGGTGAAATT GATGCCCATG AAGACAGCTT CAAATCTGCA 
GATGAATCTG GACAGGCACT GCTTGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGACCG TCCTTTCCGA GGAGAGAGCG GCGCTGCTGG AGCTGTGGGA GCTGCGCAGG 
CAGCAGTACG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GGGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAATGAAG ACTTGGGAGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTTAA GAAGCACGAA GACTTTGAGA AATCCCTTAG TGCCCAGGAG 
GAAAAGATTA CAGCATTAGA TGAATTTGCA ACCAAGCTAA TTCAGAACAA CCACTATGCA 
ATGGAAGATG TGGCCACTCG CCGAGATGCT CTGTTGAGCC GCCGCAATGC CCTTCACGAG 
AGAGCCATGC GTCGCCGGGC CCAGCTAGCC GATTCTTTCC ATCTGCAGCA GTTTTTCCGT 
GATTCTGATG AGCTCAAGAG TTGGGTCAAT GAGAAGATGA AAACTGCCAC AGATGAAGCT 
TATAAAGATC CATCCAACCT ACAAGGAAAA GTACAGAAGC ATCAGGCTTT TGAGGCTGAG 
CTCTCAGCAA ACCAGAGCCG AATTGATGCC TTGGAGAAAG CTGGCCAAAA GCTGATTGAT 
GTCAACCACT ATGCCAAGGA TGAAGTGGCA GCTCGTATGA ATGAGGTGAT CAGTTTGTGG 
AAGAAACTGC TAGAGGCCAC TGAACTGAAA GGAATAAAGC TTCGTGAAGC CAACCAGCAA 
CAGCAATTTA ATCGCAATGT TGAGGATATT GAATTGTGGC TATATGAAGT AGAAGGTCAC 
TTGGCTTCGG ATGATTACGG CAAAGATCTT ACCAATGTGC AGAACCTCCA GAAGAAACAT 
GCCCTGCTAG AGGCAGATGT GGCTGCTCAC CAGGACCGAA TTGATGGCAT CACCATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTT GATGCAGAAA ACATCAAGAA GAAACAGGAA 
GCCCTCGTGG CTCGCTATGA GGCACTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCCGATTCTC TGCGGTTGCA GCAGCTCTTC CGGGATGTTG AGGATGAGGA GACGTGGATT 
CGAGAGAAAG AGCCCATTGC CGCATCTACC AACAGAGGTA AGGATTTAAT TGGGGTCCAG 
AATCTGCTAA AGAAACATCA AGCCTTACAA GCAGAAATTG CTGGACATGA ACCACGCATC 
AAAGCAGTTA CACAGAAGGG GAATGCCATG GTGGAGGAAG GCCATTTTGC TGCAGAGGAT 
GTGAAGGCCA AGCTTCACGA GCTGAACCAA AAGTGGGAGG CACTGAAAGC CAAAGCTTCC 
CAGCGTCGGC AGGACCTGGA GGACTCTCTG CAGGCCCAGC AGTACTTTGC TGATGCTAAC 
GAGGCTGAAT CCTGGATGCG GGAGAAGGAA CCCATTGTGG GCAGCACTGA CTATGGCAAG 
GACGAAGACT CTGCTGAGGC TCTACTGAAG AAACACGAAG CTTTGATGTC AGATCTCAGT 
GCCTACGGCA GCAGCATCCA GGCTTTGCGA GAACAAGCAC AGTCCTGCCG GCAACAAGTG 
GCCCCCACGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CTCTCTACGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAG GGAGATATCC TTACCTTACT CAACAGCACC 
AACAAGGATT GGTGGAAAGT GGAAGTGAAC GATCGTCAGG GTTTTGTGCC GGCTGCGTAC 
GTGAAGAAAT TGGACCCCGC CCAGTCAGCC TCCCGGGAGA ATCTCCTGGA GGAGCAAGGC 
AGCATAGCAC TGCGGCAGGA GCAGATTGAC AATCAGACAC GCATAACTAA GGAGGCCGGC 
AGTGTATCTC TGCGTATGAA GCAGGTGGAA GAACTATATC ATTCTCTGCT GGAACTGGGT 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TTATGTTGTT CCGTGAAGCG 
AATGAACTAC AGCAATGGAT CAATGAGAAG GAAGCCGCTC TGACAAGTGA GGAGGTCGGA 
GCAGACTTGG AGCAGGTTGA GGTGCTCCAG AAGAAGTTTG ATGACTTCCA GAAGGACCTG 
AAGGCCAATG AGTCACGGTT GAAGGACATT AACAAGGTAG CTGAAGACCT GGAGTCTGAA 
GGTCTCATGG CAGAGGAGGT GCAGGCTGTG CAACAACAGG AAGTGTATGG CATGATGCCC 
AGGGATGAAA CTGATTCCAA GACAGCCTCC CCGTGGAAGT CTGCTCGTCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTACAGCAG 
CTGGCCGAGG AACGGAGCCA GCTCTTGGGC AGCGCCCATG AAGTACAGAG GTTCCACAGA 
GATGCTGATG AAACCAAAGA ATGGATTGAA GAGAAGAATC AAGCTCTAAA CACAGACAAT 
TATGGACATG ATCTCGCCAG TGTCCAGGCC CTGCAACGCA AGCATGAGGG CTTCGAGAGG 
GACCTTGCGG CTCTCGGTGA CAAGGTAAAC TCCCTTGGTG AAACAGCAGA GCGCCTGATC 
CAGTCCCATC CCGAGTCAGC AGAAGACCTG CAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGAGCAGCC TGGGGAAACG TGCAGATCAG CGCAAGGCAA AGTTGGGTGA CTCCCACGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGGGGG 
TTGGTGTCCT CAGATGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCATT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATCGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGACAGC AGCTGTTGGC TCACGGACAC TATGCCAGCC CTGAGATCAA GCAGAAACTT 
GATATTCTTG ACCAGGAGCG TGCAGACCTG GAGAAGGCCT GGGTTCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAACT GCAGCTGTTC CATCGGGACT GTGAGCAAGC TGAGAACTGG 
ATGGCTGCCC GGGAGGCCTT CTTGAATACC GAAGACAAAG GAGACTCACT GGACAGCGTA 
GAGGCTCTGA TCAAAAAACA TGAAGACTTT GACAAAGCGA TTAACGTCCA GGAAGAGAAG 
ATTGCTGCTC TGCAGGCCTT TGCCGACCAG CTCATCGCTG CCGGCCATTA TGCCAAGGGA 
GACATTTCTA GCCGGCGCAA TGAGGTCTTG GACAGGTGGC GACGTCTGAA AGCCCAGATG 
ATTGAGAAAA GGTCAAAGCT AGGAGAATCT CAAACCCTCC AACAGTTCAG CCGGGATGTG 
GATGAGATTG AGGCTTGGAT CAGTGAAAAA TTGCAAACAG CGAGTGATGA GTCGTACAAG 
GATCCCACCA ACATCCAGAG CAAGCACCAG AAGCACCAGG CTTTTGAAGC AGAGCTGCAT 
GCCAACGCTG ACCGGATCCG TGGGGTTATC GACATGGGCA ACTCCCTCAT TGAACGTGGA 
GCCTGTGCCG GCAGTGAGGA TGCTGTCAAG GCCCGCCTGG CTGCCTTAGC TGACCAGTGG 
CAGTTCTTGG TGCAAAAGTC AGCGGAAAAG AGCCAGAAAC TGAAAGAAGC CAACAAGCAG 
CAGAACTTCA ACACAGGGAT CAAGGACTTT GACTTCTGGC TGTCTGAGGT GGAGGCCCTG 
CTGGCATCCG AAGATTATGG CAAAGACCTG GCTTCTGTGA ACAACCTGCT GAAAAAGCAT 
CAACTGCTGG AAGCAGATAT ATCTGCCCAT GAGGATCGCC TGAAGGACCT GAACAGCCAG 
GCAGACAGCC TGATGACCAG CAGTGCCTTC GACACCTCCC AAGTAAAGGA CAAGAGGGAC 
ACCATCAACG GGCGCTTCCA GAAGATCAAG AGCATGGCGG CCTCCCGGCG AGCCAAGCTG 
AATGAATCCC ATCGCCTGCA CCAGTTCTTC CGGGACATGG ATGACGAGGA GTCCTGGATC 
AAGGAGAAGA AGCTGCTGGT GGGCTCAGAG GACTACGGCC GGGACCTAAC CGGCGTGCAG 
AACCTGAGGA AGAAGCACAA GCGGCTGGAA GCAGAACTGG CTGCGCATGA GCCGGCTATT 
CAGGGTGTCC TGGACACTGG CAAGAAGCTG TCCGATGACA ACACCATCGG GAAAGAGGAG 
ATCCAGCAGC GGCTGGCGCA GTTTGTGGAG CACTGGAAAG AGCTGAAGCA GCTGGCAGCT 
GCCCGGGGTC AGCGGCTGGA AGAGTCCTTG GAATATCAGC AGTTTGTAGC CAATGTGGAA 
GAGGAAGAAG CCTGGATCAA TGAGAAAATG ACCCTGGTGG CCAGCGAAGA TTATGGCGAC 
ACTCTTGCCG CCATCCAGGG CTTACTGAAG AAACATGAAG CTTTTGAGAC AGACTTCACC 
GTCCACAAGG ATCGCGTGAA TGATGTCTGC ACCAATGGAC AAGACCTCAT TAAGAAGAAC 
AATCACCATG AGGAGAACAT CTCTTCAAAG ATGAAGGGCC TGAACGGGAA AGTGTCAGAC 
CTGGAGAAAG CTGCAGCCCA GAGAAAGGCG AAGCTGGATG AGAACTCGGC CTTCCTTCAG 
TTCAACTGGA AGGCGGACGT GGTGGAGTCC TGGATCGGTG AAAAGGAGAA CAGCTTGAAG 
ACAGATGATT ATGGCCGAGA CCTGTCTTCT GTGCAGACGC TCCTCACCAA ACAGGAAACT 
TTTGACGCTG GGCTGCAGGC CTTCCAGCAG GAAGGCATTG CCAACATCAC TGCCCTCAAA 
GATCAGCTTC TCGCCGCCAA ACACGTTCAG TCCAAGGCCA TCGAGGCCCG GCACGCCTCC 
CTCATGAAGA GGTGGAGCCA GCTTCTGGCC AACTCAGCCG CCCGCAAGAA GAAGCTTCTG 
GAGGCTCAGA GTCACTTCCG CAAGGTGGAG GACCTCTTCC TGACCTTCGC CAAAAAGGCT 
TCTGCCTTCA ACAGCTGGTT TGAAAATGCA GAGGAGGACT TAACAGACCC CGTGCGCTGC 
AACTCCTTGG AAGAAATCAA AGCTTTGCGC GAGGCCCACG ACGCCTTCCG CTCCTCCCTC 
AGCTCTGCCC AGGCTGACTT CAACCAGCTG GCCGAGCTGG ACCGCCAGAT CAAGAGCTTC 
CGCGTAGCCT CCAACCCCTA CACCTGGTTT ACCATGGAGG CCCTGGAGGA GACCTGGAGG 
AACCTACAGA AAATCATCAA GGAGAGGGAG CTGGAGCTGC AGAAGGAACA GCGGCGGCAG 
GAGGAGAACG ACAAGCTGCG CCAGGAGTTT GCCCAGCACG CCAACGCCTT CCACCAGTGG 
ATCCAAGAGA CCAGGACATA CCTCCTCGAT GGGTCCTGTA TGGTGGAAGA GTCGGGGACC 
CTCGAATCCC AGCTTGAAGC TACCAAACGC AAGCACCAGG AAATCCGAGC CATGAGAAGT 
CAGCTCAAAA AGATCGAGGA CCTGGGGGCC GCCATGGAGG AGGCCCTCAT CCTGGACAAC 
AAGTACACGG AGCACAGCAC CGTGGGCCTC GCCCAGCAGT GGGACCAGCT GGACCAGCTG 
GGCATGCGCA TGCAGCACAA CCTGGAGCAG CAGATCCAGG CCAGGAACAC AACAGGTGTG 
ACTGAGGAGG CCCTCAAAGA ATTCAGCATG ATGTTTAAAC ACTTTGACAA GGACAAGTCT 
GGCAGGCTGA ACCATCAGGA GTTCAAATCT TGCCTGCGCT CCCTGGGCTA TGACCTGCCC 
ATGGTGGAGG AAGGGGAACC TGACCCTGAG TTCGAGGCAA TCCTGGACAC GGTGGATCCG 
AACAGAGATG GCCATGTCTC CTTGCAAGAA TACATGGCTT TCATGATCAG CCGCGAAACT 
GAGAACGTCA AGTCCAGCGA GGAGATTGAG AGCGCCTTCC GGGCCCTCAG CTCAGAGGGA 
AAGCCTTACG TGACCAAGGA GGAGCTCTAC CAGAACCTGA CCCGGGAACA AGCCGACTAC 
TGCGTCTCCC ACATGAAGCC CTACGTGGAC GGCAAGGGCC GCGAGCTCCC CACCGCGTTC 
GACTACGTGG AGTTCACCCG CTCGCTTTTC GTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003118.2
CDS	143..7561
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELASDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMRRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPTDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRITKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGMMPRDETDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKQKLDILDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAAGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINGRFQKIKSMAASRRAKLNESHRLHQFFRDMDDEESWIKEKKLLVGSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHASLMKRWSQLLANSAARKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	NM_003127.3
Organism	Homo sapiens(human)
Definition	Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003127.3

ORF Insert Sequence:

ATGGACCCAA GTGGGGTCAA AGTGCTGGAA ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTAG ACCGATACCA CCGCTTCAAG GAACTCTCAA CCCTTAGGCG TCAGAAGCTG 
GAAGATTCCT ATCGATTCCA GTTCTTTCAA AGAGATGCTG AAGAGCTGGA GAAATGGATA 
CAGGAAAAAC TTCAGATTGC ATCTGATGAG AATTATAAAG ACCCAACCAA CTTGCAGGGA 
AAGCTTCAGA AGCATCAAGC ATTTGAAGCT GAAGTGCAGG CCAACTCAGG AGCCATTGTT 
AAGCTGGATG AAACTGGAAA CCTGATGATC TCAGAAGGGC ATTTTGCATC TGAAACCATA 
CGGACCCGTT TGATGGAGCT GCACCGCCAG TGGGAATTAC TTTTGGAGAA GATGCGAGAA 
AAAGGAATCA AACTGCTGCA GGCCCAGAAG TTGGTGCAGT ACTTACGAGA ATGTGAGGAC 
GTGATGGACT GGATCAATGA CAAGGAAGCA ATTGTTACTT CTGAAGAGCT GGGCCAGGAT 
CTGGAGCATG TAGAGGTTTT ACAGAAGAAA TTTGAAGAGT TTCAAACAGA TATGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTGAACCAG TTTGCTGCCA AACTCATACA GGAGCAGCAC 
CCTGAGGAGG AACTGATCAA GACTAAGCAG GATGAAGTCA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CTCTGCAGAG GCAGGGGAAG CTCTTTGGGG CAGCAGAAGT TCAGCGCTTT 
AACAGGGATG TGGATGAGAC TATCAGTTGG ATTAAGGAAA AGGAGCAGTT AATGGCCTCT 
GATGATTTTG GCCGAGACCT GGCAAGTGTT CAGGCTCTGC TTCGGAAGCA CGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAAGACAAG GTCAAAGCCC TGTGTGCTGA GGCTGACCGC 
CTGCAACAGT CCCACCCTCT GAGTGCAACA CAGATTCAAG TGAAGCGAGA GGAACTGATT 
ACAAACTGGG AGCAGATCCG CACCTTGGCG GCAGAGAGAC ATGCACGGCT CAATGATTCA 
TACAGGCTTC AACGCTTCCT TGCTGACTTC CGTGACCTCA CCAGCTGGGT GACTGAGATG 
AAAGCCCTCA TCAATGCAGA TGAGCTTGCC AGTGATGTGG CTGGGGCTGA AGCCCTGCTA 
GATAGACACC AAGAGCACAA GGGTGAAATT GATGCCCATG AAGACAGCTT CAAATCTGCA 
GATGAATCTG GACAGGCACT GCTTGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGACCG TCCTTTCCGA GGAGAGAGCG GCGCTGCTGG AGCTGTGGGA GCTGCGCAGG 
CAGCAGTACG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GGGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAATGAAG ACTTGGGAGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTTAA GAAGCACGAA GACTTTGAGA AATCCCTTAG TGCCCAGGAG 
GAAAAGATTA CAGCATTAGA TGAATTTGCA ACCAAGCTAA TTCAGAACAA CCACTATGCA 
ATGGAAGATG TGGCCACTCG CCGAGATGCT CTGTTGAGCC GCCGCAATGC CCTTCACGAG 
AGAGCCATGC GTCGCCGGGC CCAGCTAGCC GATTCTTTCC ATCTGCAGCA GTTTTTCCGT 
GATTCTGATG AGCTCAAGAG TTGGGTCAAT GAGAAGATGA AAACTGCCAC AGATGAAGCT 
TATAAAGATC CATCCAACCT ACAAGGAAAA GTACAGAAGC ATCAGGCTTT TGAGGCTGAG 
CTCTCAGCAA ACCAGAGCCG AATTGATGCC TTGGAGAAAG CTGGCCAAAA GCTGATTGAT 
GTCAACCACT ATGCCAAGGA TGAAGTGGCA GCTCGTATGA ATGAGGTGAT CAGTTTGTGG 
AAGAAACTGC TAGAGGCCAC TGAACTGAAA GGAATAAAGC TTCGTGAAGC CAACCAGCAA 
CAGCAATTTA ATCGCAATGT TGAGGATATT GAATTGTGGC TATATGAAGT AGAAGGTCAC 
TTGGCTTCGG ATGATTACGG CAAAGATCTT ACCAATGTGC AGAACCTCCA GAAGAAACAT 
GCCCTGCTAG AGGCAGATGT GGCTGCTCAC CAGGACCGAA TTGATGGCAT CACCATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTT GATGCAGAAA ACATCAAGAA GAAACAGGAA 
GCCCTCGTGG CTCGCTATGA GGCACTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCCGATTCTC TGCGGTTGCA GCAGCTCTTC CGGGATGTTG AGGATGAGGA GACGTGGATT 
CGAGAGAAAG AGCCCATTGC CGCATCTACC AACAGAGGTA AGGATTTAAT TGGGGTCCAG 
AATCTGCTAA AGAAACATCA AGCCTTACAA GCAGAAATTG CTGGACATGA ACCACGCATC 
AAAGCAGTTA CACAGAAGGG GAATGCCATG GTGGAGGAAG GCCATTTTGC TGCAGAGGAT 
GTGAAGGCCA AGCTTCACGA GCTGAACCAA AAGTGGGAGG CACTGAAAGC CAAAGCTTCC 
CAGCGTCGGC AGGACCTGGA GGACTCTCTG CAGGCCCAGC AGTACTTTGC TGATGCTAAC 
GAGGCTGAAT CCTGGATGCG GGAGAAGGAA CCCATTGTGG GCAGCACTGA CTATGGCAAG 
GACGAAGACT CTGCTGAGGC TCTACTGAAG AAACACGAAG CTTTGATGTC AGATCTCAGT 
GCCTACGGCA GCAGCATCCA GGCTTTGCGA GAACAAGCAC AGTCCTGCCG GCAACAAGTG 
GCCCCCACGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CTCTCTACGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAG GGAGATATCC TTACCTTACT CAACAGCACC 
AACAAGGATT GGTGGAAAGT GGAAGTGAAC GATCGTCAGG GTTTTGTGCC GGCTGCGTAC 
GTGAAGAAAT TGGACCCCGC CCAGTCAGCC TCCCGGGAGA ATCTCCTGGA GGAGCAAGGC 
AGCATAGCAC TGCGGCAGGA GCAGATTGAC AATCAGACAC GCATAACTAA GGAGGCCGGC 
AGTGTATCTC TGCGTATGAA GCAGGTGGAA GAACTATATC ATTCTCTGCT GGAACTGGGT 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TTATGTTGTT CCGTGAAGCG 
AATGAACTAC AGCAATGGAT CAATGAGAAG GAAGCCGCTC TGACAAGTGA GGAGGTCGGA 
GCAGACTTGG AGCAGGTTGA GGTGCTCCAG AAGAAGTTTG ATGACTTCCA GAAGGACCTG 
AAGGCCAATG AGTCACGGTT GAAGGACATT AACAAGGTAG CTGAAGACCT GGAGTCTGAA 
GGTCTCATGG CAGAGGAGGT GCAGGCTGTG CAACAACAGG AAGTGTATGG CATGATGCCC 
AGGGATGAAA CTGATTCCAA GACAGCCTCC CCGTGGAAGT CTGCTCGTCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTACAGCAG 
CTGGCCGAGG AACGGAGCCA GCTCTTGGGC AGCGCCCATG AAGTACAGAG GTTCCACAGA 
GATGCTGATG AAACCAAAGA ATGGATTGAA GAGAAGAATC AAGCTCTAAA CACAGACAAT 
TATGGACATG ATCTCGCCAG TGTCCAGGCC CTGCAACGCA AGCATGAGGG CTTCGAGAGG 
GACCTTGCGG CTCTCGGTGA CAAGGTAAAC TCCCTTGGTG AAACAGCAGA GCGCCTGATC 
CAGTCCCATC CCGAGTCAGC AGAAGACCTG CAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGAGCAGCC TGGGGAAACG TGCAGATCAG CGCAAGGCAA AGTTGGGTGA CTCCCACGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGGGGG 
TTGGTGTCCT CAGATGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCATT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATCGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGACAGC AGCTGTTGGC TCACGGACAC TATGCCAGCC CTGAGATCAA GCAGAAACTT 
GATATTCTTG ACCAGGAGCG TGCAGACCTG GAGAAGGCCT GGGTTCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAACT GCAGCTGTTC CATCGGGACT GTGAGCAAGC TGAGAACTGG 
ATGGCTGCCC GGGAGGCCTT CTTGAATACC GAAGACAAAG GAGACTCACT GGACAGCGTA 
GAGGCTCTGA TCAAAAAACA TGAAGACTTT GACAAAGCGA TTAACGTCCA GGAAGAGAAG 
ATTGCTGCTC TGCAGGCCTT TGCCGACCAG CTCATCGCTG CCGGCCATTA TGCCAAGGGA 
GACATTTCTA GCCGGCGCAA TGAGGTCTTG GACAGGTGGC GACGTCTGAA AGCCCAGATG 
ATTGAGAAAA GGTCAAAGCT AGGAGAATCT CAAACCCTCC AACAGTTCAG CCGGGATGTG 
GATGAGATTG AGGCTTGGAT CAGTGAAAAA TTGCAAACAG CGAGTGATGA GTCGTACAAG 
GATCCCACCA ACATCCAGAG CAAGCACCAG AAGCACCAGG CTTTTGAAGC AGAGCTGCAT 
GCCAACGCTG ACCGGATCCG TGGGGTTATC GACATGGGCA ACTCCCTCAT TGAACGTGGA 
GCCTGTGCCG GCAGTGAGGA TGCTGTCAAG GCCCGCCTGG CTGCCTTAGC TGACCAGTGG 
CAGTTCTTGG TGCAAAAGTC AGCGGAAAAG AGCCAGAAAC TGAAAGAAGC CAACAAGCAG 
CAGAACTTCA ACACAGGGAT CAAGGACTTT GACTTCTGGC TGTCTGAGGT GGAGGCCCTG 
CTGGCATCCG AAGATTATGG CAAAGACCTG GCTTCTGTGA ACAACCTGCT GAAAAAGCAT 
CAACTGCTGG AAGCAGATAT ATCTGCCCAT GAGGATCGCC TGAAGGACCT GAACAGCCAG 
GCAGACAGCC TGATGACCAG CAGTGCCTTC GACACCTCCC AAGTAAAGGA CAAGAGGGAC 
ACCATCAACG GGCGCTTCCA GAAGATCAAG AGCATGGCGG CCTCCCGGCG AGCCAAGCTG 
AATGAATCCC ATCGCCTGCA CCAGTTCTTC CGGGACATGG ATGACGAGGA GTCCTGGATC 
AAGGAGAAGA AGCTGCTGGT GGGCTCAGAG GACTACGGCC GGGACCTAAC CGGCGTGCAG 
AACCTGAGGA AGAAGCACAA GCGGCTGGAA GCAGAACTGG CTGCGCATGA GCCGGCTATT 
CAGGGTGTCC TGGACACTGG CAAGAAGCTG TCCGATGACA ACACCATCGG GAAAGAGGAG 
ATCCAGCAGC GGCTGGCGCA GTTTGTGGAG CACTGGAAAG AGCTGAAGCA GCTGGCAGCT 
GCCCGGGGTC AGCGGCTGGA AGAGTCCTTG GAATATCAGC AGTTTGTAGC CAATGTGGAA 
GAGGAAGAAG CCTGGATCAA TGAGAAAATG ACCCTGGTGG CCAGCGAAGA TTATGGCGAC 
ACTCTTGCCG CCATCCAGGG CTTACTGAAG AAACATGAAG CTTTTGAGAC AGACTTCACC 
GTCCACAAGG ATCGCGTGAA TGATGTCTGC ACCAATGGAC AAGACCTCAT TAAGAAGAAC 
AATCACCATG AGGAGAACAT CTCTTCAAAG ATGAAGGGCC TGAACGGGAA AGTGTCAGAC 
CTGGAGAAAG CTGCAGCCCA GAGAAAGGCG AAGCTGGATG AGAACTCGGC CTTCCTTCAG 
TTCAACTGGA AGGCGGACGT GGTGGAGTCC TGGATCGGTG AAAAGGAGAA CAGCTTGAAG 
ACAGATGATT ATGGCCGAGA CCTGTCTTCT GTGCAGACGC TCCTCACCAA ACAGGAAACT 
TTTGACGCTG GGCTGCAGGC CTTCCAGCAG GAAGGCATTG CCAACATCAC TGCCCTCAAA 
GATCAGCTTC TCGCCGCCAA ACACGTTCAG TCCAAGGCCA TCGAGGCCCG GCACGCCTCC 
CTCATGAAGA GGTGGAGCCA GCTTCTGGCC AACTCAGCCG CCCGCAAGAA GAAGCTTCTG 
GAGGCTCAGA GTCACTTCCG CAAGGTGGAG GACCTCTTCC TGACCTTCGC CAAAAAGGCT 
TCTGCCTTCA ACAGCTGGTT TGAAAATGCA GAGGAGGACT TAACAGACCC CGTGCGCTGC 
AACTCCTTGG AAGAAATCAA AGCTTTGCGC GAGGCCCACG ACGCCTTCCG CTCCTCCCTC 
AGCTCTGCCC AGGCTGACTT CAACCAGCTG GCCGAGCTGG ACCGCCAGAT CAAGAGCTTC 
CGCGTAGCCT CCAACCCCTA CACCTGGTTT ACCATGGAGG CCCTGGAGGA GACCTGGAGG 
AACCTACAGA AAATCATCAA GGAGAGGGAG CTGGAGCTGC AGAAGGAACA GCGGCGGCAG 
GAGGAGAACG ACAAGCTGCG CCAGGAGTTT GCCCAGCACG CCAACGCCTT CCACCAGTGG 
ATCCAAGAGA CCAGGACATA CCTCCTCGAT GGGTCCTGTA TGGTGGAAGA GTCGGGGACC 
CTCGAATCCC AGCTTGAAGC TACCAAACGC AAGCACCAGG AAATCCGAGC CATGAGAAGT 
CAGCTCAAAA AGATCGAGGA CCTGGGGGCC GCCATGGAGG AGGCCCTCAT CCTGGACAAC 
AAGTACACGG AGCACAGCAC CGTGGGCCTC GCCCAGCAGT GGGACCAGCT GGACCAGCTG 
GGCATGCGCA TGCAGCACAA CCTGGAGCAG CAGATCCAGG CCAGGAACAC AACAGGTGTG 
ACTGAGGAGG CCCTCAAAGA ATTCAGCATG ATGTTTAAAC ACTTTGACAA GGACAAGTCT 
GGCAGGCTGA ACCATCAGGA GTTCAAATCT TGCCTGCGCT CCCTGGGCTA TGACCTGCCC 
ATGGTGGAGG AAGGGGAACC TGACCCTGAG TTCGAGGCAA TCCTGGACAC GGTGGATCCG 
AACAGAGATG GCCATGTCTC CTTGCAAGAA TACATGGCTT TCATGATCAG CCGCGAAACT 
GAGAACGTCA AGTCCAGCGA GGAGATTGAG AGCGCCTTCC GGGCCCTCAG CTCAGAGGGA 
AAGCCTTACG TGACCAAGGA GGAGCTCTAC CAGAACCTGA CCCGGGAACA AGCCGACTAC 
TGCGTCTCCC ACATGAAGCC CTACGTGGAC GGCAAGGGCC GCGAGCTCCC CACCGCGTTC 
GACTACGTGG AGTTCACCCG CTCGCTTTTC GTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( NM_001130438.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001123910.1 Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant 1, mRNA.

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CloneID	OHu22919
Clone ID Related Accession (Same CDS sequence)	NM_001130438.2
Accession Version	NM_001130438.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7434bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	spectrin alpha chain, non-erythrocytic 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC359079.1, BC034956.1, U83867.1, AL356481.16 and BC053521.1. This sequence is a reference standard in the RefSeqGene project. On Sep 15, 2010 this sequence version replaced NM_001130438.1. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC034956.1, SRR1803613.253704.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACCCAA GTGGGGTCAA AGTGCTGGAA ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTAG ACCGATACCA CCGCTTCAAG GAACTCTCAA CCCTTAGGCG TCAGAAGCTG 
GAAGATTCCT ATCGATTCCA GTTCTTTCAA AGAGATGCTG AAGAGCTGGA GAAATGGATA 
CAGGAAAAAC TTCAGATTGC ATCTGATGAG AATTATAAAG ACCCAACCAA CTTGCAGGGA 
AAGCTTCAGA AGCATCAAGC ATTTGAAGCT GAAGTGCAGG CCAACTCAGG AGCCATTGTT 
AAGCTGGATG AAACTGGAAA CCTGATGATC TCAGAAGGGC ATTTTGCATC TGAAACCATA 
CGGACCCGTT TGATGGAGCT GCACCGCCAG TGGGAATTAC TTTTGGAGAA GATGCGAGAA 
AAAGGAATCA AACTGCTGCA GGCCCAGAAG TTGGTGCAGT ACTTACGAGA ATGTGAGGAC 
GTGATGGACT GGATCAATGA CAAGGAAGCA ATTGTTACTT CTGAAGAGCT GGGCCAGGAT 
CTGGAGCATG TAGAGGTTTT ACAGAAGAAA TTTGAAGAGT TTCAAACAGA TATGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTGAACCAG TTTGCTGCCA AACTCATACA GGAGCAGCAC 
CCTGAGGAGG AACTGATCAA GACTAAGCAG GATGAAGTCA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CTCTGCAGAG GCAGGGGAAG CTCTTTGGGG CAGCAGAAGT TCAGCGCTTT 
AACAGGGATG TGGATGAGAC TATCAGTTGG ATTAAGGAAA AGGAGCAGTT AATGGCCTCT 
GATGATTTTG GCCGAGACCT GGCAAGTGTT CAGGCTCTGC TTCGGAAGCA CGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAAGACAAG GTCAAAGCCC TGTGTGCTGA GGCTGACCGC 
CTGCAACAGT CCCACCCTCT GAGTGCAACA CAGATTCAAG TGAAGCGAGA GGAACTGATT 
ACAAACTGGG AGCAGATCCG CACCTTGGCG GCAGAGAGAC ATGCACGGCT CAATGATTCA 
TACAGGCTTC AACGCTTCCT TGCTGACTTC CGTGACCTCA CCAGCTGGGT GACTGAGATG 
AAAGCCCTCA TCAATGCAGA TGAGCTTGCC AGTGATGTGG CTGGGGCTGA AGCCCTGCTA 
GATAGACACC AAGAGCACAA GGGTGAAATT GATGCCCATG AAGACAGCTT CAAATCTGCA 
GATGAATCTG GACAGGCACT GCTTGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGACCG TCCTTTCCGA GGAGAGAGCG GCGCTGCTGG AGCTGTGGGA GCTGCGCAGG 
CAGCAGTACG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GGGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAATGAAG ACTTGGGAGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTTAA GAAGCACGAA GACTTTGAGA AATCCCTTAG TGCCCAGGAG 
GAAAAGATTA CAGCATTAGA TGAATTTGCA ACCAAGCTAA TTCAGAACAA CCACTATGCA 
ATGGAAGATG TGGCCACTCG CCGAGATGCT CTGTTGAGCC GCCGCAATGC CCTTCACGAG 
AGAGCCATGC GTCGCCGGGC CCAGCTAGCC GATTCTTTCC ATCTGCAGCA GTTTTTCCGT 
GATTCTGATG AGCTCAAGAG TTGGGTCAAT GAGAAGATGA AAACTGCCAC AGATGAAGCT 
TATAAAGATC CATCCAACCT ACAAGGAAAA GTACAGAAGC ATCAGGCTTT TGAGGCTGAG 
CTCTCAGCAA ACCAGAGCCG AATTGATGCC TTGGAGAAAG CTGGCCAAAA GCTGATTGAT 
GTCAACCACT ATGCCAAGGA TGAAGTGGCA GCTCGTATGA ATGAGGTGAT CAGTTTGTGG 
AAGAAACTGC TAGAGGCCAC TGAACTGAAA GGAATAAAGC TTCGTGAAGC CAACCAGCAA 
CAGCAATTTA ATCGCAATGT TGAGGATATT GAATTGTGGC TATATGAAGT AGAAGGTCAC 
TTGGCTTCGG ATGATTACGG CAAAGATCTT ACCAATGTGC AGAACCTCCA GAAGAAACAT 
GCCCTGCTAG AGGCAGATGT GGCTGCTCAC CAGGACCGAA TTGATGGCAT CACCATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTT GATGCAGAAA ACATCAAGAA GAAACAGGAA 
GCCCTCGTGG CTCGCTATGA GGCACTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCCGATTCTC TGCGGTTGCA GCAGCTCTTC CGGGATGTTG AGGATGAGGA GACGTGGATT 
CGAGAGAAAG AGCCCATTGC CGCATCTACC AACAGAGGTA AGGATTTAAT TGGGGTCCAG 
AATCTGCTAA AGAAACATCA AGCCTTACAA GCAGAAATTG CTGGACATGA ACCACGCATC 
AAAGCAGTTA CACAGAAGGG GAATGCCATG GTGGAGGAAG GCCATTTTGC TGCAGAGGAT 
GTGAAGGCCA AGCTTCACGA GCTGAACCAA AAGTGGGAGG CACTGAAAGC CAAAGCTTCC 
CAGCGTCGGC AGGACCTGGA GGACTCTCTG CAGGCCCAGC AGTACTTTGC TGATGCTAAC 
GAGGCTGAAT CCTGGATGCG GGAGAAGGAA CCCATTGTGG GCAGCACTGA CTATGGCAAG 
GACGAAGACT CTGCTGAGGC TCTACTGAAG AAACACGAAG CTTTGATGTC AGATCTCAGT 
GCCTACGGCA GCAGCATCCA GGCTTTGCGA GAACAAGCAC AGTCCTGCCG GCAACAAGTG 
GCCCCCACGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CTCTCTACGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAG GGAGATATCC TTACCTTACT CAACAGCACC 
AACAAGGATT GGTGGAAAGT GGAAGTGAAC GATCGTCAGG GTTTTGTGCC GGCTGCGTAC 
GTGAAGAAAT TGGACCCCGC CCAGTCAGCC TCCCGGGAGA ATCTCCTGGA GGAGCAAGGC 
AGCATAGCAC TGCGGCAGGA GCAGATTGAC AATCAGACAC GCATAACTAA GGAGGCCGGC 
AGTGTATCTC TGCGTATGAA GCAGGTGGAA GAACTATATC ATTCTCTGCT GGAACTGGGT 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TTATGTTGTT CCGTGAAGCG 
AATGAACTAC AGCAATGGAT CAATGAGAAG GAAGCCGCTC TGACAAGTGA GGAGGTCGGA 
GCAGACTTGG AGCAGGTTGA GGTGCTCCAG AAGAAGTTTG ATGACTTCCA GAAGGACCTG 
AAGGCCAATG AGTCACGGTT GAAGGACATT AACAAGGTAG CTGAAGACCT GGAGTCTGAA 
GGTCTCATGG CAGAGGAGGT GCAGGCTGTG CAACAACAGG AAGTGTATGG CATGATGCCC 
AGGGATGAAA CTGATTCCAA GACAGCCTCC CCGTGGAAGT CTGCTCGTCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTACAGCAG 
CTGGCCGAGG AACGGAGCCA GCTCTTGGGC AGCGCCCATG AAGTACAGAG GTTCCACAGA 
GATGCTGATG AAACCAAAGA ATGGATTGAA GAGAAGAATC AAGCTCTAAA CACAGACAAT 
TATGGACATG ATCTCGCCAG TGTCCAGGCC CTGCAACGCA AGCATGAGGG CTTCGAGAGG 
GACCTTGCGG CTCTCGGTGA CAAGGTAAAC TCCCTTGGTG AAACAGCAGA GCGCCTGATC 
CAGTCCCATC CCGAGTCAGC AGAAGACCTG CAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGAGCAGCC TGGGGAAACG TGCAGATCAG CGCAAGGCAA AGTTGGGTGA CTCCCACGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGGGGG 
TTGGTGTCCT CAGATGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCATT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATCGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGACAGC AGCTGTTGGC TCACGGACAC TATGCCAGCC CTGAGATCAA GCAGAAACTT 
GATATTCTTG ACCAGGAGCG TGCAGACCTG GAGAAGGCCT GGGTTCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAACT GCAGCTGTTC CATCGGGACT GTGAGCAAGC TGAGAACTGG 
ATGGCTGCCC GGGAGGCCTT CTTGAATACC GAAGACAAAG GAGACTCACT GGACAGCGTA 
GAGGCTCTGA TCAAAAAACA TGAAGACTTT GACAAAGCGA TTAACGTCCA GGAAGAGAAG 
ATTGCTGCTC TGCAGGCCTT TGCCGACCAG CTCATCGCTG CCGGCCATTA TGCCAAGGGA 
GACATTTCTA GCCGGCGCAA TGAGGTCTTG GACAGGTGGC GACGTCTGAA AGCCCAGATG 
ATTGAGAAAA GGTCAAAGCT AGGAGAATCT CAAACCCTCC AACAGTTCAG CCGGGATGTG 
GATGAGATTG AGGCTTGGAT CAGTGAAAAA TTGCAAACAG CGAGTGATGA GTCGTACAAG 
GATCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ACCAGAAGCA CCAGGCTTTT 
GAAGCAGAGC TGCATGCCAA CGCTGACCGG ATCCGTGGGG TTATCGACAT GGGCAACTCC 
CTCATTGAAC GTGGAGCCTG TGCCGGCAGT GAGGATGCTG TCAAGGCCCG CCTGGCTGCC 
TTAGCTGACC AGTGGCAGTT CTTGGTGCAA AAGTCAGCGG AAAAGAGCCA GAAACTGAAA 
GAAGCCAACA AGCAGCAGAA CTTCAACACA GGGATCAAGG ACTTTGACTT CTGGCTGTCT 
GAGGTGGAGG CCCTGCTGGC ATCCGAAGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC 
CTGCTGAAAA AGCATCAACT GCTGGAAGCA GATATATCTG CCCATGAGGA TCGCCTGAAG 
GACCTGAACA GCCAGGCAGA CAGCCTGATG ACCAGCAGTG CCTTCGACAC CTCCCAAGTA 
AAGGACAAGA GGGACACCAT CAACGGGCGC TTCCAGAAGA TCAAGAGCAT GGCGGCCTCC 
CGGCGAGCCA AGCTGAATGA ATCCCATCGC CTGCACCAGT TCTTCCGGGA CATGGATGAC 
GAGGAGTCCT GGATCAAGGA GAAGAAGCTG CTGGTGGGCT CAGAGGACTA CGGCCGGGAC 
CTAACCGGCG TGCAGAACCT GAGGAAGAAG CACAAGCGGC TGGAAGCAGA ACTGGCTGCG 
CATGAGCCGG CTATTCAGGG TGTCCTGGAC ACTGGCAAGA AGCTGTCCGA TGACAACACC 
ATCGGGAAAG AGGAGATCCA GCAGCGGCTG GCGCAGTTTG TGGAGCACTG GAAAGAGCTG 
AAGCAGCTGG CAGCTGCCCG GGGTCAGCGG CTGGAAGAGT CCTTGGAATA TCAGCAGTTT 
GTAGCCAATG TGGAAGAGGA AGAAGCCTGG ATCAATGAGA AAATGACCCT GGTGGCCAGC 
GAAGATTATG GCGACACTCT TGCCGCCATC CAGGGCTTAC TGAAGAAACA TGAAGCTTTT 
GAGACAGACT TCACCGTCCA CAAGGATCGC GTGAATGATG TCTGCACCAA TGGACAAGAC 
CTCATTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CAAAGATGAA GGGCCTGAAC 
GGGAAAGTGT CAGACCTGGA GAAAGCTGCA GCCCAGAGAA AGGCGAAGCT GGATGAGAAC 
TCGGCCTTCC TTCAGTTCAA CTGGAAGGCG GACGTGGTGG AGTCCTGGAT CGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGC CGAGACCTGT CTTCTGTGCA GACGCTCCTC 
ACCAAACAGG AAACTTTTGA CGCTGGGCTG CAGGCCTTCC AGCAGGAAGG CATTGCCAAC 
ATCACTGCCC TCAAAGATCA GCTTCTCGCC GCCAAACACG TTCAGTCCAA GGCCATCGAG 
GCCCGGCACG CCTCCCTCAT GAAGAGGTGG AGCCAGCTTC TGGCCAACTC AGCCGCCCGC 
AAGAAGAAGC TTCTGGAGGC TCAGAGTCAC TTCCGCAAGG TGGAGGACCT CTTCCTGACC 
TTCGCCAAAA AGGCTTCTGC CTTCAACAGC TGGTTTGAAA ATGCAGAGGA GGACTTAACA 
GACCCCGTGC GCTGCAACTC CTTGGAAGAA ATCAAAGCTT TGCGCGAGGC CCACGACGCC 
TTCCGCTCCT CCCTCAGCTC TGCCCAGGCT GACTTCAACC AGCTGGCCGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT AGCCTCCAAC CCCTACACCT GGTTTACCAT GGAGGCCCTG 
GAGGAGACCT GGAGGAACCT ACAGAAAATC ATCAAGGAGA GGGAGCTGGA GCTGCAGAAG 
GAACAGCGGC GGCAGGAGGA GAACGACAAG CTGCGCCAGG AGTTTGCCCA GCACGCCAAC 
GCCTTCCACC AGTGGATCCA AGAGACCAGG ACATACCTCC TCGATGGGTC CTGTATGGTG 
GAAGAGTCGG GGACCCTCGA ATCCCAGCTT GAAGCTACCA AACGCAAGCA CCAGGAAATC 
CGAGCCATGA GAAGTCAGCT CAAAAAGATC GAGGACCTGG GGGCCGCCAT GGAGGAGGCC 
CTCATCCTGG ACAACAAGTA CACGGAGCAC AGCACCGTGG GCCTCGCCCA GCAGTGGGAC 
CAGCTGGACC AGCTGGGCAT GCGCATGCAG CACAACCTGG AGCAGCAGAT CCAGGCCAGG 
AACACAACAG GTGTGACTGA GGAGGCCCTC AAAGAATTCA GCATGATGTT TAAACACTTT 
GACAAGGACA AGTCTGGCAG GCTGAACCAT CAGGAGTTCA AATCTTGCCT GCGCTCCCTG 
GGCTATGACC TGCCCATGGT GGAGGAAGGG GAACCTGACC CTGAGTTCGA GGCAATCCTG 
GACACGGTGG ATCCGAACAG AGATGGCCAT GTCTCCTTGC AAGAATACAT GGCTTTCATG 
ATCAGCCGCG AAACTGAGAA CGTCAAGTCC AGCGAGGAGA TTGAGAGCGC CTTCCGGGCC 
CTCAGCTCAG AGGGAAAGCC TTACGTGACC AAGGAGGAGC TCTACCAGAA CCTGACCCGG 
GAACAAGCCG ACTACTGCGT CTCCCACATG AAGCCCTACG TGGACGGCAA GGGCCGCGAG 
CTCCCCACCG CGTTCGACTA CGTGGAGTTC ACCCGCTCGC TTTTCGTGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001123910.1
CDS	143..7576
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELASDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMRRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPTDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRITKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGMMPRDETDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKQKLDILDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAAGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINGRFQKIKSMAASRRAKLNESHRLHQFFRDMDDEESWIKEKKLLVGSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHASLMKRWSQLLANSAARKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	NM_001130438.2
Organism	Homo sapiens(human)
Definition	Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130438.2

ORF Insert Sequence:

ATGGACCCAA GTGGGGTCAA AGTGCTGGAA ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTAG ACCGATACCA CCGCTTCAAG GAACTCTCAA CCCTTAGGCG TCAGAAGCTG 
GAAGATTCCT ATCGATTCCA GTTCTTTCAA AGAGATGCTG AAGAGCTGGA GAAATGGATA 
CAGGAAAAAC TTCAGATTGC ATCTGATGAG AATTATAAAG ACCCAACCAA CTTGCAGGGA 
AAGCTTCAGA AGCATCAAGC ATTTGAAGCT GAAGTGCAGG CCAACTCAGG AGCCATTGTT 
AAGCTGGATG AAACTGGAAA CCTGATGATC TCAGAAGGGC ATTTTGCATC TGAAACCATA 
CGGACCCGTT TGATGGAGCT GCACCGCCAG TGGGAATTAC TTTTGGAGAA GATGCGAGAA 
AAAGGAATCA AACTGCTGCA GGCCCAGAAG TTGGTGCAGT ACTTACGAGA ATGTGAGGAC 
GTGATGGACT GGATCAATGA CAAGGAAGCA ATTGTTACTT CTGAAGAGCT GGGCCAGGAT 
CTGGAGCATG TAGAGGTTTT ACAGAAGAAA TTTGAAGAGT TTCAAACAGA TATGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTGAACCAG TTTGCTGCCA AACTCATACA GGAGCAGCAC 
CCTGAGGAGG AACTGATCAA GACTAAGCAG GATGAAGTCA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CTCTGCAGAG GCAGGGGAAG CTCTTTGGGG CAGCAGAAGT TCAGCGCTTT 
AACAGGGATG TGGATGAGAC TATCAGTTGG ATTAAGGAAA AGGAGCAGTT AATGGCCTCT 
GATGATTTTG GCCGAGACCT GGCAAGTGTT CAGGCTCTGC TTCGGAAGCA CGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAAGACAAG GTCAAAGCCC TGTGTGCTGA GGCTGACCGC 
CTGCAACAGT CCCACCCTCT GAGTGCAACA CAGATTCAAG TGAAGCGAGA GGAACTGATT 
ACAAACTGGG AGCAGATCCG CACCTTGGCG GCAGAGAGAC ATGCACGGCT CAATGATTCA 
TACAGGCTTC AACGCTTCCT TGCTGACTTC CGTGACCTCA CCAGCTGGGT GACTGAGATG 
AAAGCCCTCA TCAATGCAGA TGAGCTTGCC AGTGATGTGG CTGGGGCTGA AGCCCTGCTA 
GATAGACACC AAGAGCACAA GGGTGAAATT GATGCCCATG AAGACAGCTT CAAATCTGCA 
GATGAATCTG GACAGGCACT GCTTGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGACCG TCCTTTCCGA GGAGAGAGCG GCGCTGCTGG AGCTGTGGGA GCTGCGCAGG 
CAGCAGTACG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GGGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAATGAAG ACTTGGGAGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTTAA GAAGCACGAA GACTTTGAGA AATCCCTTAG TGCCCAGGAG 
GAAAAGATTA CAGCATTAGA TGAATTTGCA ACCAAGCTAA TTCAGAACAA CCACTATGCA 
ATGGAAGATG TGGCCACTCG CCGAGATGCT CTGTTGAGCC GCCGCAATGC CCTTCACGAG 
AGAGCCATGC GTCGCCGGGC CCAGCTAGCC GATTCTTTCC ATCTGCAGCA GTTTTTCCGT 
GATTCTGATG AGCTCAAGAG TTGGGTCAAT GAGAAGATGA AAACTGCCAC AGATGAAGCT 
TATAAAGATC CATCCAACCT ACAAGGAAAA GTACAGAAGC ATCAGGCTTT TGAGGCTGAG 
CTCTCAGCAA ACCAGAGCCG AATTGATGCC TTGGAGAAAG CTGGCCAAAA GCTGATTGAT 
GTCAACCACT ATGCCAAGGA TGAAGTGGCA GCTCGTATGA ATGAGGTGAT CAGTTTGTGG 
AAGAAACTGC TAGAGGCCAC TGAACTGAAA GGAATAAAGC TTCGTGAAGC CAACCAGCAA 
CAGCAATTTA ATCGCAATGT TGAGGATATT GAATTGTGGC TATATGAAGT AGAAGGTCAC 
TTGGCTTCGG ATGATTACGG CAAAGATCTT ACCAATGTGC AGAACCTCCA GAAGAAACAT 
GCCCTGCTAG AGGCAGATGT GGCTGCTCAC CAGGACCGAA TTGATGGCAT CACCATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTT GATGCAGAAA ACATCAAGAA GAAACAGGAA 
GCCCTCGTGG CTCGCTATGA GGCACTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCCGATTCTC TGCGGTTGCA GCAGCTCTTC CGGGATGTTG AGGATGAGGA GACGTGGATT 
CGAGAGAAAG AGCCCATTGC CGCATCTACC AACAGAGGTA AGGATTTAAT TGGGGTCCAG 
AATCTGCTAA AGAAACATCA AGCCTTACAA GCAGAAATTG CTGGACATGA ACCACGCATC 
AAAGCAGTTA CACAGAAGGG GAATGCCATG GTGGAGGAAG GCCATTTTGC TGCAGAGGAT 
GTGAAGGCCA AGCTTCACGA GCTGAACCAA AAGTGGGAGG CACTGAAAGC CAAAGCTTCC 
CAGCGTCGGC AGGACCTGGA GGACTCTCTG CAGGCCCAGC AGTACTTTGC TGATGCTAAC 
GAGGCTGAAT CCTGGATGCG GGAGAAGGAA CCCATTGTGG GCAGCACTGA CTATGGCAAG 
GACGAAGACT CTGCTGAGGC TCTACTGAAG AAACACGAAG CTTTGATGTC AGATCTCAGT 
GCCTACGGCA GCAGCATCCA GGCTTTGCGA GAACAAGCAC AGTCCTGCCG GCAACAAGTG 
GCCCCCACGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CTCTCTACGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAG GGAGATATCC TTACCTTACT CAACAGCACC 
AACAAGGATT GGTGGAAAGT GGAAGTGAAC GATCGTCAGG GTTTTGTGCC GGCTGCGTAC 
GTGAAGAAAT TGGACCCCGC CCAGTCAGCC TCCCGGGAGA ATCTCCTGGA GGAGCAAGGC 
AGCATAGCAC TGCGGCAGGA GCAGATTGAC AATCAGACAC GCATAACTAA GGAGGCCGGC 
AGTGTATCTC TGCGTATGAA GCAGGTGGAA GAACTATATC ATTCTCTGCT GGAACTGGGT 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TTATGTTGTT CCGTGAAGCG 
AATGAACTAC AGCAATGGAT CAATGAGAAG GAAGCCGCTC TGACAAGTGA GGAGGTCGGA 
GCAGACTTGG AGCAGGTTGA GGTGCTCCAG AAGAAGTTTG ATGACTTCCA GAAGGACCTG 
AAGGCCAATG AGTCACGGTT GAAGGACATT AACAAGGTAG CTGAAGACCT GGAGTCTGAA 
GGTCTCATGG CAGAGGAGGT GCAGGCTGTG CAACAACAGG AAGTGTATGG CATGATGCCC 
AGGGATGAAA CTGATTCCAA GACAGCCTCC CCGTGGAAGT CTGCTCGTCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTACAGCAG 
CTGGCCGAGG AACGGAGCCA GCTCTTGGGC AGCGCCCATG AAGTACAGAG GTTCCACAGA 
GATGCTGATG AAACCAAAGA ATGGATTGAA GAGAAGAATC AAGCTCTAAA CACAGACAAT 
TATGGACATG ATCTCGCCAG TGTCCAGGCC CTGCAACGCA AGCATGAGGG CTTCGAGAGG 
GACCTTGCGG CTCTCGGTGA CAAGGTAAAC TCCCTTGGTG AAACAGCAGA GCGCCTGATC 
CAGTCCCATC CCGAGTCAGC AGAAGACCTG CAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGAGCAGCC TGGGGAAACG TGCAGATCAG CGCAAGGCAA AGTTGGGTGA CTCCCACGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGGGGG 
TTGGTGTCCT CAGATGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCATT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATCGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGACAGC AGCTGTTGGC TCACGGACAC TATGCCAGCC CTGAGATCAA GCAGAAACTT 
GATATTCTTG ACCAGGAGCG TGCAGACCTG GAGAAGGCCT GGGTTCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAACT GCAGCTGTTC CATCGGGACT GTGAGCAAGC TGAGAACTGG 
ATGGCTGCCC GGGAGGCCTT CTTGAATACC GAAGACAAAG GAGACTCACT GGACAGCGTA 
GAGGCTCTGA TCAAAAAACA TGAAGACTTT GACAAAGCGA TTAACGTCCA GGAAGAGAAG 
ATTGCTGCTC TGCAGGCCTT TGCCGACCAG CTCATCGCTG CCGGCCATTA TGCCAAGGGA 
GACATTTCTA GCCGGCGCAA TGAGGTCTTG GACAGGTGGC GACGTCTGAA AGCCCAGATG 
ATTGAGAAAA GGTCAAAGCT AGGAGAATCT CAAACCCTCC AACAGTTCAG CCGGGATGTG 
GATGAGATTG AGGCTTGGAT CAGTGAAAAA TTGCAAACAG CGAGTGATGA GTCGTACAAG 
GATCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ACCAGAAGCA CCAGGCTTTT 
GAAGCAGAGC TGCATGCCAA CGCTGACCGG ATCCGTGGGG TTATCGACAT GGGCAACTCC 
CTCATTGAAC GTGGAGCCTG TGCCGGCAGT GAGGATGCTG TCAAGGCCCG CCTGGCTGCC 
TTAGCTGACC AGTGGCAGTT CTTGGTGCAA AAGTCAGCGG AAAAGAGCCA GAAACTGAAA 
GAAGCCAACA AGCAGCAGAA CTTCAACACA GGGATCAAGG ACTTTGACTT CTGGCTGTCT 
GAGGTGGAGG CCCTGCTGGC ATCCGAAGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC 
CTGCTGAAAA AGCATCAACT GCTGGAAGCA GATATATCTG CCCATGAGGA TCGCCTGAAG 
GACCTGAACA GCCAGGCAGA CAGCCTGATG ACCAGCAGTG CCTTCGACAC CTCCCAAGTA 
AAGGACAAGA GGGACACCAT CAACGGGCGC TTCCAGAAGA TCAAGAGCAT GGCGGCCTCC 
CGGCGAGCCA AGCTGAATGA ATCCCATCGC CTGCACCAGT TCTTCCGGGA CATGGATGAC 
GAGGAGTCCT GGATCAAGGA GAAGAAGCTG CTGGTGGGCT CAGAGGACTA CGGCCGGGAC 
CTAACCGGCG TGCAGAACCT GAGGAAGAAG CACAAGCGGC TGGAAGCAGA ACTGGCTGCG 
CATGAGCCGG CTATTCAGGG TGTCCTGGAC ACTGGCAAGA AGCTGTCCGA TGACAACACC 
ATCGGGAAAG AGGAGATCCA GCAGCGGCTG GCGCAGTTTG TGGAGCACTG GAAAGAGCTG 
AAGCAGCTGG CAGCTGCCCG GGGTCAGCGG CTGGAAGAGT CCTTGGAATA TCAGCAGTTT 
GTAGCCAATG TGGAAGAGGA AGAAGCCTGG ATCAATGAGA AAATGACCCT GGTGGCCAGC 
GAAGATTATG GCGACACTCT TGCCGCCATC CAGGGCTTAC TGAAGAAACA TGAAGCTTTT 
GAGACAGACT TCACCGTCCA CAAGGATCGC GTGAATGATG TCTGCACCAA TGGACAAGAC 
CTCATTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CAAAGATGAA GGGCCTGAAC 
GGGAAAGTGT CAGACCTGGA GAAAGCTGCA GCCCAGAGAA AGGCGAAGCT GGATGAGAAC 
TCGGCCTTCC TTCAGTTCAA CTGGAAGGCG GACGTGGTGG AGTCCTGGAT CGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGC CGAGACCTGT CTTCTGTGCA GACGCTCCTC 
ACCAAACAGG AAACTTTTGA CGCTGGGCTG CAGGCCTTCC AGCAGGAAGG CATTGCCAAC 
ATCACTGCCC TCAAAGATCA GCTTCTCGCC GCCAAACACG TTCAGTCCAA GGCCATCGAG 
GCCCGGCACG CCTCCCTCAT GAAGAGGTGG AGCCAGCTTC TGGCCAACTC AGCCGCCCGC 
AAGAAGAAGC TTCTGGAGGC TCAGAGTCAC TTCCGCAAGG TGGAGGACCT CTTCCTGACC 
TTCGCCAAAA AGGCTTCTGC CTTCAACAGC TGGTTTGAAA ATGCAGAGGA GGACTTAACA 
GACCCCGTGC GCTGCAACTC CTTGGAAGAA ATCAAAGCTT TGCGCGAGGC CCACGACGCC 
TTCCGCTCCT CCCTCAGCTC TGCCCAGGCT GACTTCAACC AGCTGGCCGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT AGCCTCCAAC CCCTACACCT GGTTTACCAT GGAGGCCCTG 
GAGGAGACCT GGAGGAACCT ACAGAAAATC ATCAAGGAGA GGGAGCTGGA GCTGCAGAAG 
GAACAGCGGC GGCAGGAGGA GAACGACAAG CTGCGCCAGG AGTTTGCCCA GCACGCCAAC 
GCCTTCCACC AGTGGATCCA AGAGACCAGG ACATACCTCC TCGATGGGTC CTGTATGGTG 
GAAGAGTCGG GGACCCTCGA ATCCCAGCTT GAAGCTACCA AACGCAAGCA CCAGGAAATC 
CGAGCCATGA GAAGTCAGCT CAAAAAGATC GAGGACCTGG GGGCCGCCAT GGAGGAGGCC 
CTCATCCTGG ACAACAAGTA CACGGAGCAC AGCACCGTGG GCCTCGCCCA GCAGTGGGAC 
CAGCTGGACC AGCTGGGCAT GCGCATGCAG CACAACCTGG AGCAGCAGAT CCAGGCCAGG 
AACACAACAG GTGTGACTGA GGAGGCCCTC AAAGAATTCA GCATGATGTT TAAACACTTT 
GACAAGGACA AGTCTGGCAG GCTGAACCAT CAGGAGTTCA AATCTTGCCT GCGCTCCCTG 
GGCTATGACC TGCCCATGGT GGAGGAAGGG GAACCTGACC CTGAGTTCGA GGCAATCCTG 
GACACGGTGG ATCCGAACAG AGATGGCCAT GTCTCCTTGC AAGAATACAT GGCTTTCATG 
ATCAGCCGCG AAACTGAGAA CGTCAAGTCC AGCGAGGAGA TTGAGAGCGC CTTCCGGGCC 
CTCAGCTCAG AGGGAAAGCC TTACGTGACC AAGGAGGAGC TCTACCAGAA CCTGACCCGG 
GAACAAGCCG ACTACTGCGT CTCCCACATG AAGCCCTACG TGGACGGCAA GGGCCGCGAG 
CTCCCCACCG CGTTCGACTA CGTGGAGTTC ACCCGCTCGC TTTTCGTGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( NM_001363759.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001350688.1 Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant 4, mRNA.

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CloneID	OHu43462
Clone ID Related Accession (Same CDS sequence)	NM_001363759.2 , XM_006717254.2 , NM_001363759.1
Accession Version	NM_001363759.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7497bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	spectrin alpha chain, non-erythrocytic 1 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL356481.16. On Jun 2, 2019 this sequence version replaced NM_001363759.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.77442.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END##

ATGGACCCAA GTGGGGTCAA AGTGCTGGAA ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTAG ACCGATACCA CCGCTTCAAG GAACTCTCAA CCCTTAGGCG TCAGAAGCTG 
GAAGATTCCT ATCGATTCCA GTTCTTTCAA AGAGATGCTG AAGAGCTGGA GAAATGGATA 
CAGGAAAAAC TTCAGATTGC ATCTGATGAG AATTATAAAG ACCCAACCAA CTTGCAGGGA 
AAGCTTCAGA AGCATCAAGC ATTTGAAGCT GAAGTGCAGG CCAACTCAGG AGCCATTGTT 
AAGCTGGATG AAACTGGAAA CCTGATGATC TCAGAAGGGC ATTTTGCATC TGAAACCATA 
CGGACCCGTT TGATGGAGCT GCACCGCCAG TGGGAATTAC TTTTGGAGAA GATGCGAGAA 
AAAGGAATCA AACTGCTGCA GGCCCAGAAG TTGGTGCAGT ACTTACGAGA ATGTGAGGAC 
GTGATGGACT GGATCAATGA CAAGGAAGCA ATTGTTACTT CTGAAGAGCT GGGCCAGGAT 
CTGGAGCATG TAGAGGTTTT ACAGAAGAAA TTTGAAGAGT TTCAAACAGA TATGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTGAACCAG TTTGCTGCCA AACTCATACA GGAGCAGCAC 
CCTGAGGAGG AACTGATCAA GACTAAGCAG GATGAAGTCA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CTCTGCAGAG GCAGGGGAAG CTCTTTGGGG CAGCAGAAGT TCAGCGCTTT 
AACAGGGATG TGGATGAGAC TATCAGTTGG ATTAAGGAAA AGGAGCAGTT AATGGCCTCT 
GATGATTTTG GCCGAGACCT GGCAAGTGTT CAGGCTCTGC TTCGGAAGCA CGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAAGACAAG GTCAAAGCCC TGTGTGCTGA GGCTGACCGC 
CTGCAACAGT CCCACCCTCT GAGTGCAACA CAGATTCAAG TGAAGCGAGA GGAACTGATT 
ACAAACTGGG AGCAGATCCG CACCTTGGCG GCAGAGAGAC ATGCACGGCT CAATGATTCA 
TACAGGCTTC AACGCTTCCT TGCTGACTTC CGTGACCTCA CCAGCTGGGT GACTGAGATG 
AAAGCCCTCA TCAATGCAGA TGAGCTTGCC AGTGATGTGG CTGGGGCTGA AGCCCTGCTA 
GATAGACACC AAGAGCACAA GGGTGAAATT GATGCCCATG AAGACAGCTT CAAATCTGCA 
GATGAATCTG GACAGGCACT GCTTGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGACCG TCCTTTCCGA GGAGAGAGCG GCGCTGCTGG AGCTGTGGGA GCTGCGCAGG 
CAGCAGTACG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GGGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAATGAAG ACTTGGGAGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTTAA GAAGCACGAA GACTTTGAGA AATCCCTTAG TGCCCAGGAG 
GAAAAGATTA CAGCATTAGA TGAATTTGCA ACCAAGCTAA TTCAGAACAA CCACTATGCA 
ATGGAAGATG TGGCCACTCG CCGAGATGCT CTGTTGAGCC GCCGCAATGC CCTTCACGAG 
AGAGCCATGC GTCGCCGGGC CCAGCTAGCC GATTCTTTCC ATCTGCAGCA GTTTTTCCGT 
GATTCTGATG AGCTCAAGAG TTGGGTCAAT GAGAAGATGA AAACTGCCAC AGATGAAGCT 
TATAAAGATC CATCCAACCT ACAAGGAAAA GTACAGAAGC ATCAGGCTTT TGAGGCTGAG 
CTCTCAGCAA ACCAGAGCCG AATTGATGCC TTGGAGAAAG CTGGCCAAAA GCTGATTGAT 
GTCAACCACT ATGCCAAGGA TGAAGTGGCA GCTCGTATGA ATGAGGTGAT CAGTTTGTGG 
AAGAAACTGC TAGAGGCCAC TGAACTGAAA GGAATAAAGC TTCGTGAAGC CAACCAGCAA 
CAGCAATTTA ATCGCAATGT TGAGGATATT GAATTGTGGC TATATGAAGT AGAAGGTCAC 
TTGGCTTCGG ATGATTACGG CAAAGATCTT ACCAATGTGC AGAACCTCCA GAAGAAACAT 
GCACTGCTAG AGGCAGATGT GGCTGCTCAC CAGGACCGAA TTGATGGCAT CACCATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTT GATGCAGAAA ACATCAAGAA GAAACAGGAA 
GCCCTCGTGG CTCGCTATGA GGCACTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCCGATTCTC TGCGGTTGCA GCAGCTCTTC CGGGATGTTG AGGATGAGGA GACGTGGATT 
CGAGAGAAAG AGCCCATTGC CGCATCTACC AACAGAGGTA AGGATTTAAT TGGGGTCCAG 
AATCTGCTAA AGAAACATCA AGCCTTACAA GCAGAAATTG CTGGACATGA ACCACGCATC 
AAAGCAGTTA CACAGAAGGG GAATGCCATG GTGGAGGAAG GCCATTTTGC TGCAGAGGAT 
GTGAAGGCCA AGCTTCACGA GCTGAACCAA AAGTGGGAGG CACTGAAAGC CAAAGCTTCC 
CAGCGTCGGC AGGACCTGGA GGACTCTCTG CAGGCCCAGC AGTACTTTGC TGATGCTAAC 
GAGGCTGAAT CCTGGATGCG GGAGAAGGAA CCCATTGTGG GCAGCACTGA CTATGGCAAG 
GACGAAGACT CTGCTGAGGC TCTACTGAAG AAACACGAAG CTTTGATGTC AGATCTCAGT 
GCCTACGGCA GCAGCATCCA GGCTTTGCGA GAACAAGCAC AGTCCTGCCG GCAACAAGTG 
GCCCCCACGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CTCTCTACGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAG GGAGATATCC TTACCTTACT CAACAGCACC 
AACAAGGATT GGTGGAAAGT GGAAGTGAAC GATCGTCAGG GTTTTGTGCC GGCTGCGTAC 
GTGAAGAAAT TGGACCCCGC CCAGTCAGCC TCCCGGGAGA ATCTCCTGGA GGAGCAAGGC 
AGCATAGCAC TGCGGCAGGA GCAGATTGAC AATCAGACAC GCATAACTAA GGAGGCCGGC 
AGTGTATCTC TGCGTATGAA GCAGGTGGAA GAACTATATC ATTCTCTGCT GGAACTGGGT 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TTATGTTGTT CCGTGAAGCG 
AATGAACTAC AGCAATGGAT CAATGAGAAG GAAGCCGCTC TGACAAGTGA GGAGGTCGGA 
GCAGACTTGG AGCAGGTTGA GGTGCTCCAG AAGAAGTTTG ATGACTTCCA GAAGGACCTG 
AAGGCCAATG AGTCACGGTT GAAGGACATT AACAAGGTAG CTGAAGACCT GGAGTCTGAA 
GGTCTCATGG CAGAGGAGGT GCAGGCTGTG CAACAACAGG AAGTGTATGG CATGATGCCC 
AGGGATGAAA CTGATTCCAA GACAGCCTCC CCGTGGAAGT CTGCTCGTCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTACAGCAG 
CTGGCCGAGG AACGGAGCCA GCTCTTGGGC AGCGCCCATG AAGTACAGAG GTTCCACAGA 
GATGCTGATG AAACCAAAGA ATGGATTGAA GAGAAGAATC AAGCTCTAAA CACAGACAAT 
TATGGACATG ATCTCGCCAG TGTCCAGGCC CTGCAACGCA AGCATGAGGG CTTCGAGAGG 
GACCTTGCGG CTCTCGGTGA CAAGGTAAAC TCCCTTGGTG AAACAGCAGA GCGCCTGATC 
CAGTCCCATC CCGAGTCAGC AGAAGACCTG CAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGAGCAGCC TGGGGAAACG TGCAGATCAG CGCAAGGCAA AGTTGGGTGA CTCCCACGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGGGGG 
TTGGTGTCCT CAGATGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCATT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATCGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGACAGC AGCTGTTGGC TCACGGACAC TATGCCAGCC CTGAGATCAA GCAGAAACTT 
GATATTCTTG ACCAGGAGCG TGCAGACCTG GAGAAGGCCT GGGTTCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAACT GCAGCTGTTC CATCGGGACT GTGAGCAAGC TGAGAACTGG 
ATGGCTGCCC GGGAGGCCTT CTTGAATACC GAAGACAAAG GAGACTCACT GGACAGCGTA 
GAGGCTCTGA TCAAAAAACA TGAAGACTTT GACAAAGCGA TTAACGTCCA GGAAGAGAAG 
ATTGCTGCTC TGCAGGCCTT TGCCGACCAG CTCATCGCTG CCGGCCATTA TGCCAAGGGA 
GACATTTCTA GCCGGCGCAA TGAGGTCTTG GACAGGTGGC GACGTCTGAA AGCCCAGATG 
ATTGAGAAAA GGTCAAAGCT AGGAGAATCT CAAACCCTCC AACAGTTCAG CCGGGATGTG 
GATGAGATTG AGGCTTGGAT CAGTGAAAAA TTGCAAACAG CGAGTGATGA GTCGTACAAG 
GATCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ACCAGAAGCA CCAGGCTTTT 
GAAGCAGAGC TGCATGCCAA CGCTGACCGG ATCCGTGGGG TTATCGACAT GGGCAACTCC 
CTCATTGAAC GTGGAGCCTG TGCCGGCAGT GAGGATGCTG TCAAGGCCCG CCTGGCTGCC 
TTAGCTGACC AGTGGCAGTT CTTGGTGCAA AAGTCAGCGG AAAAGAGCCA GAAACTGAAA 
GAAGCCAACA AGCAGCAGAA CTTCAACACA GGGATCAAGG ACTTTGACTT CTGGCTGTCT 
GAGGTGGAGG CCCTGCTGGC ATCCGAAGAT TATGGCAAAG ACCTAGCTTC TGTGAACAAC 
CTGCTGAAAA AGCATCAACT GCTGGAAGCA GATATATCTG CCCATGAGGA TCGCCTGAAG 
GACCTGAACA GCCAGGCAGA CAGCCTGATG ACCAGCAGTG CCTTCGACAC CTCCCAAGTA 
AAGGACAAGA GGGACACCAT CAACGGGCGC TTCCAGAAGA TCAAGAGCAT GGCGGCCTCC 
CGGCGAGCCA AGCTGAATGA ATCCCATCGC CTGCACCAGT TCTTCCGGGA CATGGATGAC 
GAGGAGTCCT GGATCAAGGA GAAGAAGCTG CTGGTGGGCT CAGAGGACTA CGGCCGGGAC 
CTAACCGGCG TGCAGAACCT GAGGAAGAAG CACAAGCGGC TGGAAGCAGA ACTGGCTGCG 
CATGAGCCGG CTATTCAGGG TGTCCTGGAC ACTGGCAAGA AGCTGTCCGA TGACAACACC 
ATCGGGAAAG AGGAGATCCA GCAGCGGCTG GCGCAGTTTG TGGAGCACTG GAAAGAGCTG 
AAGCAGCTGG CAGCTGCCCG GGGTCAGCGG CTGGAAGAGT CCTTGGAATA TCAGCAGTTT 
GTAGCCAATG TGGAAGAGGA AGAAGCCTGG ATCAATGAGA AAATGACCCT GGTGGCCAGC 
GAAGATTATG GCGACACTCT TGCCGCCATC CAGGGCTTAC TGAAGAAACA TGAAGCTTTT 
GAGACAGACT TCACCGTCCA CAAGGATCGC GTGAATGATG TCTGCACCAA TGGACAAGAC 
CTCATTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CAAAGATGAA GGGCCTGAAC 
GGGAAAGTGT CAGACCTGGA GAAAGCTGCA GCCCAGAGAA AGGCGAAGCT GGATGAGAAC 
TCGGCCTTCC TTCAGTTCAA CTGGAAGGCG GACGTGGTGG AGTCCTGGAT CGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGC CGAGACCTGT CTTCTGTGCA GACGCTCCTC 
ACCAAACAGG AAACTTTTGA CGCTGGGCTG CAGGCCTTCC AGCAGGAAGG CATTGCCAAC 
ATCACTGCCC TCAAAGATCA GCTTCTCGCC GCCAAACACG TTCAGTCCAA GGCCATCGAG 
GCCCGGCACG CCTCCCTCAT GAAGAGGTGG AGCCAGCTTC TGGCCAACTC AGCCGCCCGC 
AAGAAGAAGC TTCTGGAGGC TCAGAGTCAC TTCCGCAAGG TGGAGGACCT CTTCCTGACC 
TTCGCCAAAA AGGCTTCTGC CTTCAACAGC TGGTTTGAAA ATGCAGAGGA GGACTTAACA 
GACCCCGTGC GCTGCAACTC CTTGGAAGAA ATCAAAGCTT TGCGCGAGGC CCACGACGCC 
TTCCGCTCCT CCCTCAGCTC TGCCCAGGCT GACTTCAACC AGCTGGCCGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT AGCCTCCAAC CCCTACACCT GGTTTACCAT GGAGGCCCTG 
GAGGAGACCT GGAGGAACCT ACAGAAAATC ATCAAGGAGA GGGAGCTGGA GCTGCAGAAG 
GAACAGCGGC GGCAGGAGGA GAACGACAAG CTGCGCCAGG AGTTTGCCCA GCACGCCAAC 
GCCTTCCACC AGTGGATCCA AGAGACCAGG ACATACCTCC TCGATGGCAT AGCATATCGT 
CGGGTCATTC GTGTCTATCA GTATGAAGTT GGGGATGATC TGTCTGGAAG GTCCTGTATG 
GTGGAAGAGT CGGGGACCCT CGAATCCCAG CTTGAAGCTA CCAAACGCAA GCACCAGGAA 
ATCCGAGCCA TGAGAAGTCA GCTCAAAAAG ATCGAGGACC TGGGGGCCGC CATGGAGGAG 
GCCCTCATCC TGGACAACAA GTACACGGAG CACAGCACCG TGGGCCTCGC CCAGCAGTGG 
GACCAGCTGG ACCAGCTGGG CATGCGCATG CAGCACAACC TGGAGCAGCA GATCCAGGCC 
AGGAACACAA CAGGTGTGAC TGAGGAGGCC CTCAAAGAAT TCAGCATGAT GTTTAAACAC 
TTTGACAAGG ACAAGTCTGG CAGGCTGAAC CATCAGGAGT TCAAATCTTG CCTGCGCTCC 
CTGGGCTATG ACCTGCCCAT GGTGGAGGAA GGGGAACCTG ACCCTGAGTT CGAGGCAATC 
CTGGACACGG TGGATCCGAA CAGAGATGGC CATGTCTCCT TGCAAGAATA CATGGCTTTC 
ATGATCAGCC GCGAAACTGA GAACGTCAAG TCCAGCGAGG AGATTGAGAG CGCCTTCCGG 
GCCCTCAGCT CAGAGGGAAA GCCTTACGTG ACCAAGGAGG AGCTCTACCA GAACCTGACC 
CGGGAACAAG CCGACTACTG CGTCTCCCAC ATGAAGCCCT ACGTGGACGG CAAGGGCCGC 
GAGCTCCCCA CCGCGTTCGA CTACGTGGAG TTCACCCGCT CGCTTTTCGT GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350688.1
CDS	114..7610
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELASDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMRRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPTDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRITKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGMMPRDETDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKQKLDILDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAAGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINGRFQKIKSMAASRRAKLNESHRLHQFFRDMDDEESWIKEKKLLVGSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHASLMKRWSQLLANSAARKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGIAYRRVIRVYQYEVGDDLSGRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	NM_001363759.2
Organism	Homo sapiens(human)
Definition	Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363759.2

ORF Insert Sequence:

ATGGACCCAA GTGGGGTCAA AGTGCTGGAA ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTAG ACCGATACCA CCGCTTCAAG GAACTCTCAA CCCTTAGGCG TCAGAAGCTG 
GAAGATTCCT ATCGATTCCA GTTCTTTCAA AGAGATGCTG AAGAGCTGGA GAAATGGATA 
CAGGAAAAAC TTCAGATTGC ATCTGATGAG AATTATAAAG ACCCAACCAA CTTGCAGGGA 
AAGCTTCAGA AGCATCAAGC ATTTGAAGCT GAAGTGCAGG CCAACTCAGG AGCCATTGTT 
AAGCTGGATG AAACTGGAAA CCTGATGATC TCAGAAGGGC ATTTTGCATC TGAAACCATA 
CGGACCCGTT TGATGGAGCT GCACCGCCAG TGGGAATTAC TTTTGGAGAA GATGCGAGAA 
AAAGGAATCA AACTGCTGCA GGCCCAGAAG TTGGTGCAGT ACTTACGAGA ATGTGAGGAC 
GTGATGGACT GGATCAATGA CAAGGAAGCA ATTGTTACTT CTGAAGAGCT GGGCCAGGAT 
CTGGAGCATG TAGAGGTTTT ACAGAAGAAA TTTGAAGAGT TTCAAACAGA TATGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTGAACCAG TTTGCTGCCA AACTCATACA GGAGCAGCAC 
CCTGAGGAGG AACTGATCAA GACTAAGCAG GATGAAGTCA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CTCTGCAGAG GCAGGGGAAG CTCTTTGGGG CAGCAGAAGT TCAGCGCTTT 
AACAGGGATG TGGATGAGAC TATCAGTTGG ATTAAGGAAA AGGAGCAGTT AATGGCCTCT 
GATGATTTTG GCCGAGACCT GGCAAGTGTT CAGGCTCTGC TTCGGAAGCA CGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAAGACAAG GTCAAAGCCC TGTGTGCTGA GGCTGACCGC 
CTGCAACAGT CCCACCCTCT GAGTGCAACA CAGATTCAAG TGAAGCGAGA GGAACTGATT 
ACAAACTGGG AGCAGATCCG CACCTTGGCG GCAGAGAGAC ATGCACGGCT CAATGATTCA 
TACAGGCTTC AACGCTTCCT TGCTGACTTC CGTGACCTCA CCAGCTGGGT GACTGAGATG 
AAAGCCCTCA TCAATGCAGA TGAGCTTGCC AGTGATGTGG CTGGGGCTGA AGCCCTGCTA 
GATAGACACC AAGAGCACAA GGGTGAAATT GATGCCCATG AAGACAGCTT CAAATCTGCA 
GATGAATCTG GACAGGCACT GCTTGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGACCG TCCTTTCCGA GGAGAGAGCG GCGCTGCTGG AGCTGTGGGA GCTGCGCAGG 
CAGCAGTACG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GGGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAATGAAG ACTTGGGAGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTTAA GAAGCACGAA GACTTTGAGA AATCCCTTAG TGCCCAGGAG 
GAAAAGATTA CAGCATTAGA TGAATTTGCA ACCAAGCTAA TTCAGAACAA CCACTATGCA 
ATGGAAGATG TGGCCACTCG CCGAGATGCT CTGTTGAGCC GCCGCAATGC CCTTCACGAG 
AGAGCCATGC GTCGCCGGGC CCAGCTAGCC GATTCTTTCC ATCTGCAGCA GTTTTTCCGT 
GATTCTGATG AGCTCAAGAG TTGGGTCAAT GAGAAGATGA AAACTGCCAC AGATGAAGCT 
TATAAAGATC CATCCAACCT ACAAGGAAAA GTACAGAAGC ATCAGGCTTT TGAGGCTGAG 
CTCTCAGCAA ACCAGAGCCG AATTGATGCC TTGGAGAAAG CTGGCCAAAA GCTGATTGAT 
GTCAACCACT ATGCCAAGGA TGAAGTGGCA GCTCGTATGA ATGAGGTGAT CAGTTTGTGG 
AAGAAACTGC TAGAGGCCAC TGAACTGAAA GGAATAAAGC TTCGTGAAGC CAACCAGCAA 
CAGCAATTTA ATCGCAATGT TGAGGATATT GAATTGTGGC TATATGAAGT AGAAGGTCAC 
TTGGCTTCGG ATGATTACGG CAAAGATCTT ACCAATGTGC AGAACCTCCA GAAGAAACAT 
GCACTGCTAG AGGCAGATGT GGCTGCTCAC CAGGACCGAA TTGATGGCAT CACCATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTT GATGCAGAAA ACATCAAGAA GAAACAGGAA 
GCCCTCGTGG CTCGCTATGA GGCACTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCCGATTCTC TGCGGTTGCA GCAGCTCTTC CGGGATGTTG AGGATGAGGA GACGTGGATT 
CGAGAGAAAG AGCCCATTGC CGCATCTACC AACAGAGGTA AGGATTTAAT TGGGGTCCAG 
AATCTGCTAA AGAAACATCA AGCCTTACAA GCAGAAATTG CTGGACATGA ACCACGCATC 
AAAGCAGTTA CACAGAAGGG GAATGCCATG GTGGAGGAAG GCCATTTTGC TGCAGAGGAT 
GTGAAGGCCA AGCTTCACGA GCTGAACCAA AAGTGGGAGG CACTGAAAGC CAAAGCTTCC 
CAGCGTCGGC AGGACCTGGA GGACTCTCTG CAGGCCCAGC AGTACTTTGC TGATGCTAAC 
GAGGCTGAAT CCTGGATGCG GGAGAAGGAA CCCATTGTGG GCAGCACTGA CTATGGCAAG 
GACGAAGACT CTGCTGAGGC TCTACTGAAG AAACACGAAG CTTTGATGTC AGATCTCAGT 
GCCTACGGCA GCAGCATCCA GGCTTTGCGA GAACAAGCAC AGTCCTGCCG GCAACAAGTG 
GCCCCCACGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CTCTCTACGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAG GGAGATATCC TTACCTTACT CAACAGCACC 
AACAAGGATT GGTGGAAAGT GGAAGTGAAC GATCGTCAGG GTTTTGTGCC GGCTGCGTAC 
GTGAAGAAAT TGGACCCCGC CCAGTCAGCC TCCCGGGAGA ATCTCCTGGA GGAGCAAGGC 
AGCATAGCAC TGCGGCAGGA GCAGATTGAC AATCAGACAC GCATAACTAA GGAGGCCGGC 
AGTGTATCTC TGCGTATGAA GCAGGTGGAA GAACTATATC ATTCTCTGCT GGAACTGGGT 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TTATGTTGTT CCGTGAAGCG 
AATGAACTAC AGCAATGGAT CAATGAGAAG GAAGCCGCTC TGACAAGTGA GGAGGTCGGA 
GCAGACTTGG AGCAGGTTGA GGTGCTCCAG AAGAAGTTTG ATGACTTCCA GAAGGACCTG 
AAGGCCAATG AGTCACGGTT GAAGGACATT AACAAGGTAG CTGAAGACCT GGAGTCTGAA 
GGTCTCATGG CAGAGGAGGT GCAGGCTGTG CAACAACAGG AAGTGTATGG CATGATGCCC 
AGGGATGAAA CTGATTCCAA GACAGCCTCC CCGTGGAAGT CTGCTCGTCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTACAGCAG 
CTGGCCGAGG AACGGAGCCA GCTCTTGGGC AGCGCCCATG AAGTACAGAG GTTCCACAGA 
GATGCTGATG AAACCAAAGA ATGGATTGAA GAGAAGAATC AAGCTCTAAA CACAGACAAT 
TATGGACATG ATCTCGCCAG TGTCCAGGCC CTGCAACGCA AGCATGAGGG CTTCGAGAGG 
GACCTTGCGG CTCTCGGTGA CAAGGTAAAC TCCCTTGGTG AAACAGCAGA GCGCCTGATC 
CAGTCCCATC CCGAGTCAGC AGAAGACCTG CAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGAGCAGCC TGGGGAAACG TGCAGATCAG CGCAAGGCAA AGTTGGGTGA CTCCCACGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGGGGG 
TTGGTGTCCT CAGATGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCATT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATCGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGACAGC AGCTGTTGGC TCACGGACAC TATGCCAGCC CTGAGATCAA GCAGAAACTT 
GATATTCTTG ACCAGGAGCG TGCAGACCTG GAGAAGGCCT GGGTTCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAACT GCAGCTGTTC CATCGGGACT GTGAGCAAGC TGAGAACTGG 
ATGGCTGCCC GGGAGGCCTT CTTGAATACC GAAGACAAAG GAGACTCACT GGACAGCGTA 
GAGGCTCTGA TCAAAAAACA TGAAGACTTT GACAAAGCGA TTAACGTCCA GGAAGAGAAG 
ATTGCTGCTC TGCAGGCCTT TGCCGACCAG CTCATCGCTG CCGGCCATTA TGCCAAGGGA 
GACATTTCTA GCCGGCGCAA TGAGGTCTTG GACAGGTGGC GACGTCTGAA AGCCCAGATG 
ATTGAGAAAA GGTCAAAGCT AGGAGAATCT CAAACCCTCC AACAGTTCAG CCGGGATGTG 
GATGAGATTG AGGCTTGGAT CAGTGAAAAA TTGCAAACAG CGAGTGATGA GTCGTACAAG 
GATCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ACCAGAAGCA CCAGGCTTTT 
GAAGCAGAGC TGCATGCCAA CGCTGACCGG ATCCGTGGGG TTATCGACAT GGGCAACTCC 
CTCATTGAAC GTGGAGCCTG TGCCGGCAGT GAGGATGCTG TCAAGGCCCG CCTGGCTGCC 
TTAGCTGACC AGTGGCAGTT CTTGGTGCAA AAGTCAGCGG AAAAGAGCCA GAAACTGAAA 
GAAGCCAACA AGCAGCAGAA CTTCAACACA GGGATCAAGG ACTTTGACTT CTGGCTGTCT 
GAGGTGGAGG CCCTGCTGGC ATCCGAAGAT TATGGCAAAG ACCTAGCTTC TGTGAACAAC 
CTGCTGAAAA AGCATCAACT GCTGGAAGCA GATATATCTG CCCATGAGGA TCGCCTGAAG 
GACCTGAACA GCCAGGCAGA CAGCCTGATG ACCAGCAGTG CCTTCGACAC CTCCCAAGTA 
AAGGACAAGA GGGACACCAT CAACGGGCGC TTCCAGAAGA TCAAGAGCAT GGCGGCCTCC 
CGGCGAGCCA AGCTGAATGA ATCCCATCGC CTGCACCAGT TCTTCCGGGA CATGGATGAC 
GAGGAGTCCT GGATCAAGGA GAAGAAGCTG CTGGTGGGCT CAGAGGACTA CGGCCGGGAC 
CTAACCGGCG TGCAGAACCT GAGGAAGAAG CACAAGCGGC TGGAAGCAGA ACTGGCTGCG 
CATGAGCCGG CTATTCAGGG TGTCCTGGAC ACTGGCAAGA AGCTGTCCGA TGACAACACC 
ATCGGGAAAG AGGAGATCCA GCAGCGGCTG GCGCAGTTTG TGGAGCACTG GAAAGAGCTG 
AAGCAGCTGG CAGCTGCCCG GGGTCAGCGG CTGGAAGAGT CCTTGGAATA TCAGCAGTTT 
GTAGCCAATG TGGAAGAGGA AGAAGCCTGG ATCAATGAGA AAATGACCCT GGTGGCCAGC 
GAAGATTATG GCGACACTCT TGCCGCCATC CAGGGCTTAC TGAAGAAACA TGAAGCTTTT 
GAGACAGACT TCACCGTCCA CAAGGATCGC GTGAATGATG TCTGCACCAA TGGACAAGAC 
CTCATTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CAAAGATGAA GGGCCTGAAC 
GGGAAAGTGT CAGACCTGGA GAAAGCTGCA GCCCAGAGAA AGGCGAAGCT GGATGAGAAC 
TCGGCCTTCC TTCAGTTCAA CTGGAAGGCG GACGTGGTGG AGTCCTGGAT CGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGC CGAGACCTGT CTTCTGTGCA GACGCTCCTC 
ACCAAACAGG AAACTTTTGA CGCTGGGCTG CAGGCCTTCC AGCAGGAAGG CATTGCCAAC 
ATCACTGCCC TCAAAGATCA GCTTCTCGCC GCCAAACACG TTCAGTCCAA GGCCATCGAG 
GCCCGGCACG CCTCCCTCAT GAAGAGGTGG AGCCAGCTTC TGGCCAACTC AGCCGCCCGC 
AAGAAGAAGC TTCTGGAGGC TCAGAGTCAC TTCCGCAAGG TGGAGGACCT CTTCCTGACC 
TTCGCCAAAA AGGCTTCTGC CTTCAACAGC TGGTTTGAAA ATGCAGAGGA GGACTTAACA 
GACCCCGTGC GCTGCAACTC CTTGGAAGAA ATCAAAGCTT TGCGCGAGGC CCACGACGCC 
TTCCGCTCCT CCCTCAGCTC TGCCCAGGCT GACTTCAACC AGCTGGCCGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT AGCCTCCAAC CCCTACACCT GGTTTACCAT GGAGGCCCTG 
GAGGAGACCT GGAGGAACCT ACAGAAAATC ATCAAGGAGA GGGAGCTGGA GCTGCAGAAG 
GAACAGCGGC GGCAGGAGGA GAACGACAAG CTGCGCCAGG AGTTTGCCCA GCACGCCAAC 
GCCTTCCACC AGTGGATCCA AGAGACCAGG ACATACCTCC TCGATGGCAT AGCATATCGT 
CGGGTCATTC GTGTCTATCA GTATGAAGTT GGGGATGATC TGTCTGGAAG GTCCTGTATG 
GTGGAAGAGT CGGGGACCCT CGAATCCCAG CTTGAAGCTA CCAAACGCAA GCACCAGGAA 
ATCCGAGCCA TGAGAAGTCA GCTCAAAAAG ATCGAGGACC TGGGGGCCGC CATGGAGGAG 
GCCCTCATCC TGGACAACAA GTACACGGAG CACAGCACCG TGGGCCTCGC CCAGCAGTGG 
GACCAGCTGG ACCAGCTGGG CATGCGCATG CAGCACAACC TGGAGCAGCA GATCCAGGCC 
AGGAACACAA CAGGTGTGAC TGAGGAGGCC CTCAAAGAAT TCAGCATGAT GTTTAAACAC 
TTTGACAAGG ACAAGTCTGG CAGGCTGAAC CATCAGGAGT TCAAATCTTG CCTGCGCTCC 
CTGGGCTATG ACCTGCCCAT GGTGGAGGAA GGGGAACCTG ACCCTGAGTT CGAGGCAATC 
CTGGACACGG TGGATCCGAA CAGAGATGGC CATGTCTCCT TGCAAGAATA CATGGCTTTC 
ATGATCAGCC GCGAAACTGA GAACGTCAAG TCCAGCGAGG AGATTGAGAG CGCCTTCCGG 
GCCCTCAGCT CAGAGGGAAA GCCTTACGTG ACCAAGGAGG AGCTCTACCA GAACCTGACC 
CGGGAACAAG CCGACTACTG CGTCTCCCAC ATGAAGCCCT ACGTGGACGG CAAGGGCCGC 
GAGCTCCCCA CCGCGTTCGA CTACGTGGAG TTCACCCGCT CGCTTTTCGT GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( NM_001363759.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001350688.1 Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant 4, mRNA.

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CloneID	OHu43462
Clone ID Related Accession (Same CDS sequence)	NM_001363759.2 , XM_006717254.2 , NM_001363759.1
Accession Version	NM_001363759.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7497bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-27
Organism	Homo sapiens(human)
Product	spectrin alpha chain, non-erythrocytic 1 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL356481.16. On May 29, 2018 this sequence version replaced XM_006717254.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.77442.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END##

ATGGACCCAA GTGGGGTCAA AGTGCTGGAA ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTAG ACCGATACCA CCGCTTCAAG GAACTCTCAA CCCTTAGGCG TCAGAAGCTG 
GAAGATTCCT ATCGATTCCA GTTCTTTCAA AGAGATGCTG AAGAGCTGGA GAAATGGATA 
CAGGAAAAAC TTCAGATTGC ATCTGATGAG AATTATAAAG ACCCAACCAA CTTGCAGGGA 
AAGCTTCAGA AGCATCAAGC ATTTGAAGCT GAAGTGCAGG CCAACTCAGG AGCCATTGTT 
AAGCTGGATG AAACTGGAAA CCTGATGATC TCAGAAGGGC ATTTTGCATC TGAAACCATA 
CGGACCCGTT TGATGGAGCT GCACCGCCAG TGGGAATTAC TTTTGGAGAA GATGCGAGAA 
AAAGGAATCA AACTGCTGCA GGCCCAGAAG TTGGTGCAGT ACTTACGAGA ATGTGAGGAC 
GTGATGGACT GGATCAATGA CAAGGAAGCA ATTGTTACTT CTGAAGAGCT GGGCCAGGAT 
CTGGAGCATG TAGAGGTTTT ACAGAAGAAA TTTGAAGAGT TTCAAACAGA TATGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTGAACCAG TTTGCTGCCA AACTCATACA GGAGCAGCAC 
CCTGAGGAGG AACTGATCAA GACTAAGCAG GATGAAGTCA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CTCTGCAGAG GCAGGGGAAG CTCTTTGGGG CAGCAGAAGT TCAGCGCTTT 
AACAGGGATG TGGATGAGAC TATCAGTTGG ATTAAGGAAA AGGAGCAGTT AATGGCCTCT 
GATGATTTTG GCCGAGACCT GGCAAGTGTT CAGGCTCTGC TTCGGAAGCA CGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAAGACAAG GTCAAAGCCC TGTGTGCTGA GGCTGACCGC 
CTGCAACAGT CCCACCCTCT GAGTGCAACA CAGATTCAAG TGAAGCGAGA GGAACTGATT 
ACAAACTGGG AGCAGATCCG CACCTTGGCG GCAGAGAGAC ATGCACGGCT CAATGATTCA 
TACAGGCTTC AACGCTTCCT TGCTGACTTC CGTGACCTCA CCAGCTGGGT GACTGAGATG 
AAAGCCCTCA TCAATGCAGA TGAGCTTGCC AGTGATGTGG CTGGGGCTGA AGCCCTGCTA 
GATAGACACC AAGAGCACAA GGGTGAAATT GATGCCCATG AAGACAGCTT CAAATCTGCA 
GATGAATCTG GACAGGCACT GCTTGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGACCG TCCTTTCCGA GGAGAGAGCG GCGCTGCTGG AGCTGTGGGA GCTGCGCAGG 
CAGCAGTACG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GGGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAATGAAG ACTTGGGAGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTTAA GAAGCACGAA GACTTTGAGA AATCCCTTAG TGCCCAGGAG 
GAAAAGATTA CAGCATTAGA TGAATTTGCA ACCAAGCTAA TTCAGAACAA CCACTATGCA 
ATGGAAGATG TGGCCACTCG CCGAGATGCT CTGTTGAGCC GCCGCAATGC CCTTCACGAG 
AGAGCCATGC GTCGCCGGGC CCAGCTAGCC GATTCTTTCC ATCTGCAGCA GTTTTTCCGT 
GATTCTGATG AGCTCAAGAG TTGGGTCAAT GAGAAGATGA AAACTGCCAC AGATGAAGCT 
TATAAAGATC CATCCAACCT ACAAGGAAAA GTACAGAAGC ATCAGGCTTT TGAGGCTGAG 
CTCTCAGCAA ACCAGAGCCG AATTGATGCC TTGGAGAAAG CTGGCCAAAA GCTGATTGAT 
GTCAACCACT ATGCCAAGGA TGAAGTGGCA GCTCGTATGA ATGAGGTGAT CAGTTTGTGG 
AAGAAACTGC TAGAGGCCAC TGAACTGAAA GGAATAAAGC TTCGTGAAGC CAACCAGCAA 
CAGCAATTTA ATCGCAATGT TGAGGATATT GAATTGTGGC TATATGAAGT AGAAGGTCAC 
TTGGCTTCGG ATGATTACGG CAAAGATCTT ACCAATGTGC AGAACCTCCA GAAGAAACAT 
GCACTGCTAG AGGCAGATGT GGCTGCTCAC CAGGACCGAA TTGATGGCAT CACCATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTT GATGCAGAAA ACATCAAGAA GAAACAGGAA 
GCCCTCGTGG CTCGCTATGA GGCACTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCCGATTCTC TGCGGTTGCA GCAGCTCTTC CGGGATGTTG AGGATGAGGA GACGTGGATT 
CGAGAGAAAG AGCCCATTGC CGCATCTACC AACAGAGGTA AGGATTTAAT TGGGGTCCAG 
AATCTGCTAA AGAAACATCA AGCCTTACAA GCAGAAATTG CTGGACATGA ACCACGCATC 
AAAGCAGTTA CACAGAAGGG GAATGCCATG GTGGAGGAAG GCCATTTTGC TGCAGAGGAT 
GTGAAGGCCA AGCTTCACGA GCTGAACCAA AAGTGGGAGG CACTGAAAGC CAAAGCTTCC 
CAGCGTCGGC AGGACCTGGA GGACTCTCTG CAGGCCCAGC AGTACTTTGC TGATGCTAAC 
GAGGCTGAAT CCTGGATGCG GGAGAAGGAA CCCATTGTGG GCAGCACTGA CTATGGCAAG 
GACGAAGACT CTGCTGAGGC TCTACTGAAG AAACACGAAG CTTTGATGTC AGATCTCAGT 
GCCTACGGCA GCAGCATCCA GGCTTTGCGA GAACAAGCAC AGTCCTGCCG GCAACAAGTG 
GCCCCCACGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CTCTCTACGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAG GGAGATATCC TTACCTTACT CAACAGCACC 
AACAAGGATT GGTGGAAAGT GGAAGTGAAC GATCGTCAGG GTTTTGTGCC GGCTGCGTAC 
GTGAAGAAAT TGGACCCCGC CCAGTCAGCC TCCCGGGAGA ATCTCCTGGA GGAGCAAGGC 
AGCATAGCAC TGCGGCAGGA GCAGATTGAC AATCAGACAC GCATAACTAA GGAGGCCGGC 
AGTGTATCTC TGCGTATGAA GCAGGTGGAA GAACTATATC ATTCTCTGCT GGAACTGGGT 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TTATGTTGTT CCGTGAAGCG 
AATGAACTAC AGCAATGGAT CAATGAGAAG GAAGCCGCTC TGACAAGTGA GGAGGTCGGA 
GCAGACTTGG AGCAGGTTGA GGTGCTCCAG AAGAAGTTTG ATGACTTCCA GAAGGACCTG 
AAGGCCAATG AGTCACGGTT GAAGGACATT AACAAGGTAG CTGAAGACCT GGAGTCTGAA 
GGTCTCATGG CAGAGGAGGT GCAGGCTGTG CAACAACAGG AAGTGTATGG CATGATGCCC 
AGGGATGAAA CTGATTCCAA GACAGCCTCC CCGTGGAAGT CTGCTCGTCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTACAGCAG 
CTGGCCGAGG AACGGAGCCA GCTCTTGGGC AGCGCCCATG AAGTACAGAG GTTCCACAGA 
GATGCTGATG AAACCAAAGA ATGGATTGAA GAGAAGAATC AAGCTCTAAA CACAGACAAT 
TATGGACATG ATCTCGCCAG TGTCCAGGCC CTGCAACGCA AGCATGAGGG CTTCGAGAGG 
GACCTTGCGG CTCTCGGTGA CAAGGTAAAC TCCCTTGGTG AAACAGCAGA GCGCCTGATC 
CAGTCCCATC CCGAGTCAGC AGAAGACCTG CAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGAGCAGCC TGGGGAAACG TGCAGATCAG CGCAAGGCAA AGTTGGGTGA CTCCCACGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGGGGG 
TTGGTGTCCT CAGATGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCATT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATCGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGACAGC AGCTGTTGGC TCACGGACAC TATGCCAGCC CTGAGATCAA GCAGAAACTT 
GATATTCTTG ACCAGGAGCG TGCAGACCTG GAGAAGGCCT GGGTTCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAACT GCAGCTGTTC CATCGGGACT GTGAGCAAGC TGAGAACTGG 
ATGGCTGCCC GGGAGGCCTT CTTGAATACC GAAGACAAAG GAGACTCACT GGACAGCGTA 
GAGGCTCTGA TCAAAAAACA TGAAGACTTT GACAAAGCGA TTAACGTCCA GGAAGAGAAG 
ATTGCTGCTC TGCAGGCCTT TGCCGACCAG CTCATCGCTG CCGGCCATTA TGCCAAGGGA 
GACATTTCTA GCCGGCGCAA TGAGGTCTTG GACAGGTGGC GACGTCTGAA AGCCCAGATG 
ATTGAGAAAA GGTCAAAGCT AGGAGAATCT CAAACCCTCC AACAGTTCAG CCGGGATGTG 
GATGAGATTG AGGCTTGGAT CAGTGAAAAA TTGCAAACAG CGAGTGATGA GTCGTACAAG 
GATCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ACCAGAAGCA CCAGGCTTTT 
GAAGCAGAGC TGCATGCCAA CGCTGACCGG ATCCGTGGGG TTATCGACAT GGGCAACTCC 
CTCATTGAAC GTGGAGCCTG TGCCGGCAGT GAGGATGCTG TCAAGGCCCG CCTGGCTGCC 
TTAGCTGACC AGTGGCAGTT CTTGGTGCAA AAGTCAGCGG AAAAGAGCCA GAAACTGAAA 
GAAGCCAACA AGCAGCAGAA CTTCAACACA GGGATCAAGG ACTTTGACTT CTGGCTGTCT 
GAGGTGGAGG CCCTGCTGGC ATCCGAAGAT TATGGCAAAG ACCTAGCTTC TGTGAACAAC 
CTGCTGAAAA AGCATCAACT GCTGGAAGCA GATATATCTG CCCATGAGGA TCGCCTGAAG 
GACCTGAACA GCCAGGCAGA CAGCCTGATG ACCAGCAGTG CCTTCGACAC CTCCCAAGTA 
AAGGACAAGA GGGACACCAT CAACGGGCGC TTCCAGAAGA TCAAGAGCAT GGCGGCCTCC 
CGGCGAGCCA AGCTGAATGA ATCCCATCGC CTGCACCAGT TCTTCCGGGA CATGGATGAC 
GAGGAGTCCT GGATCAAGGA GAAGAAGCTG CTGGTGGGCT CAGAGGACTA CGGCCGGGAC 
CTAACCGGCG TGCAGAACCT GAGGAAGAAG CACAAGCGGC TGGAAGCAGA ACTGGCTGCG 
CATGAGCCGG CTATTCAGGG TGTCCTGGAC ACTGGCAAGA AGCTGTCCGA TGACAACACC 
ATCGGGAAAG AGGAGATCCA GCAGCGGCTG GCGCAGTTTG TGGAGCACTG GAAAGAGCTG 
AAGCAGCTGG CAGCTGCCCG GGGTCAGCGG CTGGAAGAGT CCTTGGAATA TCAGCAGTTT 
GTAGCCAATG TGGAAGAGGA AGAAGCCTGG ATCAATGAGA AAATGACCCT GGTGGCCAGC 
GAAGATTATG GCGACACTCT TGCCGCCATC CAGGGCTTAC TGAAGAAACA TGAAGCTTTT 
GAGACAGACT TCACCGTCCA CAAGGATCGC GTGAATGATG TCTGCACCAA TGGACAAGAC 
CTCATTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CAAAGATGAA GGGCCTGAAC 
GGGAAAGTGT CAGACCTGGA GAAAGCTGCA GCCCAGAGAA AGGCGAAGCT GGATGAGAAC 
TCGGCCTTCC TTCAGTTCAA CTGGAAGGCG GACGTGGTGG AGTCCTGGAT CGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGC CGAGACCTGT CTTCTGTGCA GACGCTCCTC 
ACCAAACAGG AAACTTTTGA CGCTGGGCTG CAGGCCTTCC AGCAGGAAGG CATTGCCAAC 
ATCACTGCCC TCAAAGATCA GCTTCTCGCC GCCAAACACG TTCAGTCCAA GGCCATCGAG 
GCCCGGCACG CCTCCCTCAT GAAGAGGTGG AGCCAGCTTC TGGCCAACTC AGCCGCCCGC 
AAGAAGAAGC TTCTGGAGGC TCAGAGTCAC TTCCGCAAGG TGGAGGACCT CTTCCTGACC 
TTCGCCAAAA AGGCTTCTGC CTTCAACAGC TGGTTTGAAA ATGCAGAGGA GGACTTAACA 
GACCCCGTGC GCTGCAACTC CTTGGAAGAA ATCAAAGCTT TGCGCGAGGC CCACGACGCC 
TTCCGCTCCT CCCTCAGCTC TGCCCAGGCT GACTTCAACC AGCTGGCCGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT AGCCTCCAAC CCCTACACCT GGTTTACCAT GGAGGCCCTG 
GAGGAGACCT GGAGGAACCT ACAGAAAATC ATCAAGGAGA GGGAGCTGGA GCTGCAGAAG 
GAACAGCGGC GGCAGGAGGA GAACGACAAG CTGCGCCAGG AGTTTGCCCA GCACGCCAAC 
GCCTTCCACC AGTGGATCCA AGAGACCAGG ACATACCTCC TCGATGGCAT AGCATATCGT 
CGGGTCATTC GTGTCTATCA GTATGAAGTT GGGGATGATC TGTCTGGAAG GTCCTGTATG 
GTGGAAGAGT CGGGGACCCT CGAATCCCAG CTTGAAGCTA CCAAACGCAA GCACCAGGAA 
ATCCGAGCCA TGAGAAGTCA GCTCAAAAAG ATCGAGGACC TGGGGGCCGC CATGGAGGAG 
GCCCTCATCC TGGACAACAA GTACACGGAG CACAGCACCG TGGGCCTCGC CCAGCAGTGG 
GACCAGCTGG ACCAGCTGGG CATGCGCATG CAGCACAACC TGGAGCAGCA GATCCAGGCC 
AGGAACACAA CAGGTGTGAC TGAGGAGGCC CTCAAAGAAT TCAGCATGAT GTTTAAACAC 
TTTGACAAGG ACAAGTCTGG CAGGCTGAAC CATCAGGAGT TCAAATCTTG CCTGCGCTCC 
CTGGGCTATG ACCTGCCCAT GGTGGAGGAA GGGGAACCTG ACCCTGAGTT CGAGGCAATC 
CTGGACACGG TGGATCCGAA CAGAGATGGC CATGTCTCCT TGCAAGAATA CATGGCTTTC 
ATGATCAGCC GCGAAACTGA GAACGTCAAG TCCAGCGAGG AGATTGAGAG CGCCTTCCGG 
GCCCTCAGCT CAGAGGGAAA GCCTTACGTG ACCAAGGAGG AGCTCTACCA GAACCTGACC 
CGGGAACAAG CCGACTACTG CGTCTCCCAC ATGAAGCCCT ACGTGGACGG CAAGGGCCGC 
GAGCTCCCCA CCGCGTTCGA CTACGTGGAG TTCACCCGCT CGCTTTTCGT GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350688.1
CDS	114..7610
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELASDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMRRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPTDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRITKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGMMPRDETDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKQKLDILDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAAGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINGRFQKIKSMAASRRAKLNESHRLHQFFRDMDDEESWIKEKKLLVGSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHASLMKRWSQLLANSAARKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGIAYRRVIRVYQYEVGDDLSGRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	NM_001363759.1
Organism	Homo sapiens(human)
Definition	Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363759.1

ORF Insert Sequence:

ATGGACCCAA GTGGGGTCAA AGTGCTGGAA ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTAG ACCGATACCA CCGCTTCAAG GAACTCTCAA CCCTTAGGCG TCAGAAGCTG 
GAAGATTCCT ATCGATTCCA GTTCTTTCAA AGAGATGCTG AAGAGCTGGA GAAATGGATA 
CAGGAAAAAC TTCAGATTGC ATCTGATGAG AATTATAAAG ACCCAACCAA CTTGCAGGGA 
AAGCTTCAGA AGCATCAAGC ATTTGAAGCT GAAGTGCAGG CCAACTCAGG AGCCATTGTT 
AAGCTGGATG AAACTGGAAA CCTGATGATC TCAGAAGGGC ATTTTGCATC TGAAACCATA 
CGGACCCGTT TGATGGAGCT GCACCGCCAG TGGGAATTAC TTTTGGAGAA GATGCGAGAA 
AAAGGAATCA AACTGCTGCA GGCCCAGAAG TTGGTGCAGT ACTTACGAGA ATGTGAGGAC 
GTGATGGACT GGATCAATGA CAAGGAAGCA ATTGTTACTT CTGAAGAGCT GGGCCAGGAT 
CTGGAGCATG TAGAGGTTTT ACAGAAGAAA TTTGAAGAGT TTCAAACAGA TATGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTGAACCAG TTTGCTGCCA AACTCATACA GGAGCAGCAC 
CCTGAGGAGG AACTGATCAA GACTAAGCAG GATGAAGTCA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CTCTGCAGAG GCAGGGGAAG CTCTTTGGGG CAGCAGAAGT TCAGCGCTTT 
AACAGGGATG TGGATGAGAC TATCAGTTGG ATTAAGGAAA AGGAGCAGTT AATGGCCTCT 
GATGATTTTG GCCGAGACCT GGCAAGTGTT CAGGCTCTGC TTCGGAAGCA CGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAAGACAAG GTCAAAGCCC TGTGTGCTGA GGCTGACCGC 
CTGCAACAGT CCCACCCTCT GAGTGCAACA CAGATTCAAG TGAAGCGAGA GGAACTGATT 
ACAAACTGGG AGCAGATCCG CACCTTGGCG GCAGAGAGAC ATGCACGGCT CAATGATTCA 
TACAGGCTTC AACGCTTCCT TGCTGACTTC CGTGACCTCA CCAGCTGGGT GACTGAGATG 
AAAGCCCTCA TCAATGCAGA TGAGCTTGCC AGTGATGTGG CTGGGGCTGA AGCCCTGCTA 
GATAGACACC AAGAGCACAA GGGTGAAATT GATGCCCATG AAGACAGCTT CAAATCTGCA 
GATGAATCTG GACAGGCACT GCTTGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGACCG TCCTTTCCGA GGAGAGAGCG GCGCTGCTGG AGCTGTGGGA GCTGCGCAGG 
CAGCAGTACG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GGGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAATGAAG ACTTGGGAGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTTAA GAAGCACGAA GACTTTGAGA AATCCCTTAG TGCCCAGGAG 
GAAAAGATTA CAGCATTAGA TGAATTTGCA ACCAAGCTAA TTCAGAACAA CCACTATGCA 
ATGGAAGATG TGGCCACTCG CCGAGATGCT CTGTTGAGCC GCCGCAATGC CCTTCACGAG 
AGAGCCATGC GTCGCCGGGC CCAGCTAGCC GATTCTTTCC ATCTGCAGCA GTTTTTCCGT 
GATTCTGATG AGCTCAAGAG TTGGGTCAAT GAGAAGATGA AAACTGCCAC AGATGAAGCT 
TATAAAGATC CATCCAACCT ACAAGGAAAA GTACAGAAGC ATCAGGCTTT TGAGGCTGAG 
CTCTCAGCAA ACCAGAGCCG AATTGATGCC TTGGAGAAAG CTGGCCAAAA GCTGATTGAT 
GTCAACCACT ATGCCAAGGA TGAAGTGGCA GCTCGTATGA ATGAGGTGAT CAGTTTGTGG 
AAGAAACTGC TAGAGGCCAC TGAACTGAAA GGAATAAAGC TTCGTGAAGC CAACCAGCAA 
CAGCAATTTA ATCGCAATGT TGAGGATATT GAATTGTGGC TATATGAAGT AGAAGGTCAC 
TTGGCTTCGG ATGATTACGG CAAAGATCTT ACCAATGTGC AGAACCTCCA GAAGAAACAT 
GCACTGCTAG AGGCAGATGT GGCTGCTCAC CAGGACCGAA TTGATGGCAT CACCATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTT GATGCAGAAA ACATCAAGAA GAAACAGGAA 
GCCCTCGTGG CTCGCTATGA GGCACTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCCGATTCTC TGCGGTTGCA GCAGCTCTTC CGGGATGTTG AGGATGAGGA GACGTGGATT 
CGAGAGAAAG AGCCCATTGC CGCATCTACC AACAGAGGTA AGGATTTAAT TGGGGTCCAG 
AATCTGCTAA AGAAACATCA AGCCTTACAA GCAGAAATTG CTGGACATGA ACCACGCATC 
AAAGCAGTTA CACAGAAGGG GAATGCCATG GTGGAGGAAG GCCATTTTGC TGCAGAGGAT 
GTGAAGGCCA AGCTTCACGA GCTGAACCAA AAGTGGGAGG CACTGAAAGC CAAAGCTTCC 
CAGCGTCGGC AGGACCTGGA GGACTCTCTG CAGGCCCAGC AGTACTTTGC TGATGCTAAC 
GAGGCTGAAT CCTGGATGCG GGAGAAGGAA CCCATTGTGG GCAGCACTGA CTATGGCAAG 
GACGAAGACT CTGCTGAGGC TCTACTGAAG AAACACGAAG CTTTGATGTC AGATCTCAGT 
GCCTACGGCA GCAGCATCCA GGCTTTGCGA GAACAAGCAC AGTCCTGCCG GCAACAAGTG 
GCCCCCACGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CTCTCTACGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAG GGAGATATCC TTACCTTACT CAACAGCACC 
AACAAGGATT GGTGGAAAGT GGAAGTGAAC GATCGTCAGG GTTTTGTGCC GGCTGCGTAC 
GTGAAGAAAT TGGACCCCGC CCAGTCAGCC TCCCGGGAGA ATCTCCTGGA GGAGCAAGGC 
AGCATAGCAC TGCGGCAGGA GCAGATTGAC AATCAGACAC GCATAACTAA GGAGGCCGGC 
AGTGTATCTC TGCGTATGAA GCAGGTGGAA GAACTATATC ATTCTCTGCT GGAACTGGGT 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TTATGTTGTT CCGTGAAGCG 
AATGAACTAC AGCAATGGAT CAATGAGAAG GAAGCCGCTC TGACAAGTGA GGAGGTCGGA 
GCAGACTTGG AGCAGGTTGA GGTGCTCCAG AAGAAGTTTG ATGACTTCCA GAAGGACCTG 
AAGGCCAATG AGTCACGGTT GAAGGACATT AACAAGGTAG CTGAAGACCT GGAGTCTGAA 
GGTCTCATGG CAGAGGAGGT GCAGGCTGTG CAACAACAGG AAGTGTATGG CATGATGCCC 
AGGGATGAAA CTGATTCCAA GACAGCCTCC CCGTGGAAGT CTGCTCGTCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTACAGCAG 
CTGGCCGAGG AACGGAGCCA GCTCTTGGGC AGCGCCCATG AAGTACAGAG GTTCCACAGA 
GATGCTGATG AAACCAAAGA ATGGATTGAA GAGAAGAATC AAGCTCTAAA CACAGACAAT 
TATGGACATG ATCTCGCCAG TGTCCAGGCC CTGCAACGCA AGCATGAGGG CTTCGAGAGG 
GACCTTGCGG CTCTCGGTGA CAAGGTAAAC TCCCTTGGTG AAACAGCAGA GCGCCTGATC 
CAGTCCCATC CCGAGTCAGC AGAAGACCTG CAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGAGCAGCC TGGGGAAACG TGCAGATCAG CGCAAGGCAA AGTTGGGTGA CTCCCACGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGGGGG 
TTGGTGTCCT CAGATGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCATT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATCGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGACAGC AGCTGTTGGC TCACGGACAC TATGCCAGCC CTGAGATCAA GCAGAAACTT 
GATATTCTTG ACCAGGAGCG TGCAGACCTG GAGAAGGCCT GGGTTCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAACT GCAGCTGTTC CATCGGGACT GTGAGCAAGC TGAGAACTGG 
ATGGCTGCCC GGGAGGCCTT CTTGAATACC GAAGACAAAG GAGACTCACT GGACAGCGTA 
GAGGCTCTGA TCAAAAAACA TGAAGACTTT GACAAAGCGA TTAACGTCCA GGAAGAGAAG 
ATTGCTGCTC TGCAGGCCTT TGCCGACCAG CTCATCGCTG CCGGCCATTA TGCCAAGGGA 
GACATTTCTA GCCGGCGCAA TGAGGTCTTG GACAGGTGGC GACGTCTGAA AGCCCAGATG 
ATTGAGAAAA GGTCAAAGCT AGGAGAATCT CAAACCCTCC AACAGTTCAG CCGGGATGTG 
GATGAGATTG AGGCTTGGAT CAGTGAAAAA TTGCAAACAG CGAGTGATGA GTCGTACAAG 
GATCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ACCAGAAGCA CCAGGCTTTT 
GAAGCAGAGC TGCATGCCAA CGCTGACCGG ATCCGTGGGG TTATCGACAT GGGCAACTCC 
CTCATTGAAC GTGGAGCCTG TGCCGGCAGT GAGGATGCTG TCAAGGCCCG CCTGGCTGCC 
TTAGCTGACC AGTGGCAGTT CTTGGTGCAA AAGTCAGCGG AAAAGAGCCA GAAACTGAAA 
GAAGCCAACA AGCAGCAGAA CTTCAACACA GGGATCAAGG ACTTTGACTT CTGGCTGTCT 
GAGGTGGAGG CCCTGCTGGC ATCCGAAGAT TATGGCAAAG ACCTAGCTTC TGTGAACAAC 
CTGCTGAAAA AGCATCAACT GCTGGAAGCA GATATATCTG CCCATGAGGA TCGCCTGAAG 
GACCTGAACA GCCAGGCAGA CAGCCTGATG ACCAGCAGTG CCTTCGACAC CTCCCAAGTA 
AAGGACAAGA GGGACACCAT CAACGGGCGC TTCCAGAAGA TCAAGAGCAT GGCGGCCTCC 
CGGCGAGCCA AGCTGAATGA ATCCCATCGC CTGCACCAGT TCTTCCGGGA CATGGATGAC 
GAGGAGTCCT GGATCAAGGA GAAGAAGCTG CTGGTGGGCT CAGAGGACTA CGGCCGGGAC 
CTAACCGGCG TGCAGAACCT GAGGAAGAAG CACAAGCGGC TGGAAGCAGA ACTGGCTGCG 
CATGAGCCGG CTATTCAGGG TGTCCTGGAC ACTGGCAAGA AGCTGTCCGA TGACAACACC 
ATCGGGAAAG AGGAGATCCA GCAGCGGCTG GCGCAGTTTG TGGAGCACTG GAAAGAGCTG 
AAGCAGCTGG CAGCTGCCCG GGGTCAGCGG CTGGAAGAGT CCTTGGAATA TCAGCAGTTT 
GTAGCCAATG TGGAAGAGGA AGAAGCCTGG ATCAATGAGA AAATGACCCT GGTGGCCAGC 
GAAGATTATG GCGACACTCT TGCCGCCATC CAGGGCTTAC TGAAGAAACA TGAAGCTTTT 
GAGACAGACT TCACCGTCCA CAAGGATCGC GTGAATGATG TCTGCACCAA TGGACAAGAC 
CTCATTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CAAAGATGAA GGGCCTGAAC 
GGGAAAGTGT CAGACCTGGA GAAAGCTGCA GCCCAGAGAA AGGCGAAGCT GGATGAGAAC 
TCGGCCTTCC TTCAGTTCAA CTGGAAGGCG GACGTGGTGG AGTCCTGGAT CGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGC CGAGACCTGT CTTCTGTGCA GACGCTCCTC 
ACCAAACAGG AAACTTTTGA CGCTGGGCTG CAGGCCTTCC AGCAGGAAGG CATTGCCAAC 
ATCACTGCCC TCAAAGATCA GCTTCTCGCC GCCAAACACG TTCAGTCCAA GGCCATCGAG 
GCCCGGCACG CCTCCCTCAT GAAGAGGTGG AGCCAGCTTC TGGCCAACTC AGCCGCCCGC 
AAGAAGAAGC TTCTGGAGGC TCAGAGTCAC TTCCGCAAGG TGGAGGACCT CTTCCTGACC 
TTCGCCAAAA AGGCTTCTGC CTTCAACAGC TGGTTTGAAA ATGCAGAGGA GGACTTAACA 
GACCCCGTGC GCTGCAACTC CTTGGAAGAA ATCAAAGCTT TGCGCGAGGC CCACGACGCC 
TTCCGCTCCT CCCTCAGCTC TGCCCAGGCT GACTTCAACC AGCTGGCCGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT AGCCTCCAAC CCCTACACCT GGTTTACCAT GGAGGCCCTG 
GAGGAGACCT GGAGGAACCT ACAGAAAATC ATCAAGGAGA GGGAGCTGGA GCTGCAGAAG 
GAACAGCGGC GGCAGGAGGA GAACGACAAG CTGCGCCAGG AGTTTGCCCA GCACGCCAAC 
GCCTTCCACC AGTGGATCCA AGAGACCAGG ACATACCTCC TCGATGGCAT AGCATATCGT 
CGGGTCATTC GTGTCTATCA GTATGAAGTT GGGGATGATC TGTCTGGAAG GTCCTGTATG 
GTGGAAGAGT CGGGGACCCT CGAATCCCAG CTTGAAGCTA CCAAACGCAA GCACCAGGAA 
ATCCGAGCCA TGAGAAGTCA GCTCAAAAAG ATCGAGGACC TGGGGGCCGC CATGGAGGAG 
GCCCTCATCC TGGACAACAA GTACACGGAG CACAGCACCG TGGGCCTCGC CCAGCAGTGG 
GACCAGCTGG ACCAGCTGGG CATGCGCATG CAGCACAACC TGGAGCAGCA GATCCAGGCC 
AGGAACACAA CAGGTGTGAC TGAGGAGGCC CTCAAAGAAT TCAGCATGAT GTTTAAACAC 
TTTGACAAGG ACAAGTCTGG CAGGCTGAAC CATCAGGAGT TCAAATCTTG CCTGCGCTCC 
CTGGGCTATG ACCTGCCCAT GGTGGAGGAA GGGGAACCTG ACCCTGAGTT CGAGGCAATC 
CTGGACACGG TGGATCCGAA CAGAGATGGC CATGTCTCCT TGCAAGAATA CATGGCTTTC 
ATGATCAGCC GCGAAACTGA GAACGTCAAG TCCAGCGAGG AGATTGAGAG CGCCTTCCGG 
GCCCTCAGCT CAGAGGGAAA GCCTTACGTG ACCAAGGAGG AGCTCTACCA GAACCTGACC 
CGGGAACAAG CCGACTACTG CGTCTCCCAC ATGAAGCCCT ACGTGGACGG CAAGGGCCGC 
GAGCTCCCCA CCGCGTTCGA CTACGTGGAG TTCACCCGCT CGCTTTTCGT GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_006717245.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_006717308.1 Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X1, mRNA.

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CloneID	OHu43453
Clone ID Related Accession (Same CDS sequence)	NM_001375318.1 , XM_006717245.1
Accession Version	XM_006717245.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7533bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-12
Organism	Homo sapiens(human)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END##

ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACCG ATACCACCGC 
TTCAAGGAAC TCTCAACCCT TAGGCGTCAG AAGCTGGAAG ATTCCTATCG ATTCCAGTTC 
TTTCAAAGAG ATGCTGAAGA GCTGGAGAAA TGGATACAGG AAAAACTTCA GATTGCATCT 
GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGAAAGC TTCAGAAGCA TCAAGCATTT 
GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAGC TGGATGAAAC TGGAAACCTG 
ATGATCTCAG AAGGGCATTT TGCATCTGAA ACCATACGGA CCCGTTTGAT GGAGCTGCAC 
CGCCAGTGGG AATTACTTTT GGAGAAGATG CGAGAAAAAG GAATCAAACT GCTGCAGGCC 
CAGAAGTTGG TGCAGTACTT ACGAGAATGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCAATTG TTACTTCTGA AGAGCTGGGC CAGGATCTGG AGCATGTAGA GGTTTTACAG 
AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCTCATG AAGAAAGAGT TAATGAAGTG 
AACCAGTTTG CTGCCAAACT CATACAGGAG CAGCACCCTG AGGAGGAACT GATCAAGACT 
AAGCAGGATG AAGTCAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCTCT GCAGAGGCAG 
GGGAAGCTCT TTGGGGCAGC AGAAGTTCAG CGCTTTAACA GGGATGTGGA TGAGACTATC 
AGTTGGATTA AGGAAAAGGA GCAGTTAATG GCCTCTGATG ATTTTGGCCG AGACCTGGCA 
AGTGTTCAGG CTCTGCTTCG GAAGCACGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAA 
GACAAGGTCA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCCCA CCCTCTGAGT 
GCAACACAGA TTCAAGTGAA GCGAGAGGAA CTGATTACAA ACTGGGAGCA GATCCGCACC 
TTGGCGGCAG AGAGACATGC ACGGCTCAAT GATTCATACA GGCTTCAACG CTTCCTTGCT 
GACTTCCGTG ACCTCACCAG CTGGGTGACT GAGATGAAAG CCCTCATCAA TGCAGATGAG 
CTTGCCAGTG ATGTGGCTGG GGCTGAAGCC CTGCTAGATA GACACCAAGA GCACAAGGGT 
GAAATTGATG CCCATGAAGA CAGCTTCAAA TCTGCAGATG AATCTGGACA GGCACTGCTT 
GCTGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCGTCCT TTCCGAGGAG 
AGAGCGGCGC TGCTGGAGCT GTGGGAGCTG CGCAGGCAGC AGTACGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGGGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ATGAAGACTT GGGAGATTCC TTGGATAGTG TGGAAGCGCT TCTTAAGAAG 
CACGAAGACT TTGAGAAATC CCTTAGTGCC CAGGAGGAAA AGATTACAGC ATTAGATGAA 
TTTGCAACCA AGCTAATTCA GAACAACCAC TATGCAATGG AAGATGTGGC CACTCGCCGA 
GATGCTCTGT TGAGCCGCCG CAATGCCCTT CACGAGAGAG CCATGCGTCG CCGGGCCCAG 
CTAGCCGATT CTTTCCATCT GCAGCAGTTT TTCCGTGATT CTGATGAGCT CAAGAGTTGG 
GTCAATGAGA AGATGAAAAC TGCCACAGAT GAAGCTTATA AAGATCCATC CAACCTACAA 
GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CAGCAAACCA GAGCCGAATT 
GATGCCTTGG AGAAAGCTGG CCAAAAGCTG ATTGATGTCA ACCACTATGC CAAGGATGAA 
GTGGCAGCTC GTATGAATGA GGTGATCAGT TTGTGGAAGA AACTGCTAGA GGCCACTGAA 
CTGAAAGGAA TAAAGCTTCG TGAAGCCAAC CAGCAACAGC AATTTAATCG CAATGTTGAG 
GATATTGAAT TGTGGCTATA TGAAGTAGAA GGTCACTTGG CTTCGGATGA TTACGGCAAA 
GATCTTACCA ATGTGCAGAA CCTCCAGAAG AAACATGCAC TGCTAGAGGC AGATGTGGCT 
GCTCACCAGG ACCGAATTGA TGGCATCACC ATTCAGGCCC GCCAGTTCCA AGATGCTGGC 
CATTTTGATG CAGAAAACAT CAAGAAGAAA CAGGAAGCCC TCGTGGCTCG CTATGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCTCTGCG GTTGCAGCAG 
CTCTTCCGGG ATGTTGAGGA TGAGGAGACG TGGATTCGAG AGAAAGAGCC CATTGCCGCA 
TCTACCAACA GAGGTAAGGA TTTAATTGGG GTCCAGAATC TGCTAAAGAA ACATCAAGCC 
TTACAAGCAG AAATTGCTGG ACATGAACCA CGCATCAAAG CAGTTACACA GAAGGGGAAT 
GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAGCT TCACGAGCTG 
AACCAAAAGT GGGAGGCACT GAAAGCCAAA GCTTCCCAGC GTCGGCAGGA CCTGGAGGAC 
TCTCTGCAGG CCCAGCAGTA CTTTGCTGAT GCTAACGAGG CTGAATCCTG GATGCGGGAG 
AAGGAACCCA TTGTGGGCAG CACTGACTAT GGCAAGGACG AAGACTCTGC TGAGGCTCTA 
CTGAAGAAAC ACGAAGCTTT GATGTCAGAT CTCAGTGCCT ACGGCAGCAG CATCCAGGCT 
TTGCGAGAAC AAGCACAGTC CTGCCGGCAA CAAGTGGCCC CCACGGATGA TGAGACTGGG 
AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAGGGAG ATATCCTTAC CTTACTCAAC AGCACCAACA AGGATTGGTG GAAAGTGGAA 
GTGAACGATC GTCAGGGTTT TGTGCCGGCT GCGTACGTGA AGAAATTGGA CCCCGCCCAG 
TCAGCCTCCC GGGAGAATCT CCTGGAGGAG CAAGGCAGCA TAGCACTGCG GCAGGAGCAG 
ATTGACAATC AGACACGCAT AACTAAGGAG GCCGGCAGTG TATCTCTGCG TATGAAGCAG 
GTGGAAGAAC TATATCATTC TCTGCTGGAA CTGGGTGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGTTGCA AGAAGTTTAT GTTGTTCCGT GAAGCGAATG AACTACAGCA ATGGATCAAT 
GAGAAGGAAG CCGCTCTGAC AAGTGAGGAG GTCGGAGCAG ACTTGGAGCA GGTTGAGGTG 
CTCCAGAAGA AGTTTGATGA CTTCCAGAAG GACCTGAAGG CCAATGAGTC ACGGTTGAAG 
GACATTAACA AGGTAGCTGA AGACCTGGAG TCTGAAGGTC TCATGGCAGA GGAGGTGCAG 
GCTGTGCAAC AACAGGAAGT GTATGGCATG ATGCCCAGGG ATGAAACTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGTCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTGA ATGAGCGCTG GCGGTCCCTA CAGCAGCTGG CCGAGGAACG GAGCCAGCTC 
TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CTGATGAAAC CAAAGAATGG 
ATTGAAGAGA AGAATCAAGC TCTAAACACA GACAATTATG GACATGATCT CGCCAGTGTC 
CAGGCCCTGC AACGCAAGCA TGAGGGCTTC GAGAGGGACC TTGCGGCTCT CGGTGACAAG 
GTAAACTCCC TTGGTGAAAC AGCAGAGCGC CTGATCCAGT CCCATCCCGA GTCAGCAGAA 
GACCTGCAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA GCAGCCTGGG GAAACGTGCA 
GATCAGCGCA AGGCAAAGTT GGGTGACTCC CACGACCTGC AGCGCTTCCT TAGCGATTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATA CGGGGGTTGG TGTCCTCAGA TGAGCTAGCC 
AAGGATGTCA CCGGAGCTGA GGCATTGCTG GAGCGACACC AGGAACACCG GACAGAAATC 
GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GACAGCAGCT GTTGGCTCAC 
GGACACTATG CCAGCCCTGA GATCAAGCAG AAACTTGATA TTCTTGACCA GGAGCGTGCA 
GACCTGGAGA AGGCCTGGGT TCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAACTGCAG 
CTGTTCCATC GGGACTGTGA GCAAGCTGAG AACTGGATGG CTGCCCGGGA GGCCTTCTTG 
AATACCGAAG ACAAAGGAGA CTCACTGGAC AGCGTAGAGG CTCTGATCAA AAAACATGAA 
GACTTTGACA AAGCGATTAA CGTCCAGGAA GAGAAGATTG CTGCTCTGCA GGCCTTTGCC 
GACCAGCTCA TCGCTGCCGG CCATTATGCC AAGGGAGACA TTTCTAGCCG GCGCAATGAG 
GTCTTGGACA GGTGGCGACG TCTGAAAGCC CAGATGATTG AGAAAAGGTC AAAGCTAGGA 
GAATCTCAAA CCCTCCAACA GTTCAGCCGG GATGTGGATG AGATTGAGGC TTGGATCAGT 
GAAAAATTGC AAACAGCGAG TGATGAGTCG TACAAGGATC CCACCAACAT CCAGCTTTCC 
AAGCTGCTGA GCAAGCACCA GAAGCACCAG GCTTTTGAAG CAGAGCTGCA TGCCAACGCT 
GACCGGATCC GTGGGGTTAT CGACATGGGC AACTCCCTCA TTGAACGTGG AGCCTGTGCC 
GGCAGTGAGG ATGCTGTCAA GGCCCGCCTG GCTGCCTTAG CTGACCAGTG GCAGTTCTTG 
GTGCAAAAGT CAGCGGAAAA GAGCCAGAAA CTGAAAGAAG CCAACAAGCA GCAGAACTTC 
AACACAGGGA TCAAGGACTT TGACTTCTGG CTGTCTGAGG TGGAGGCCCT GCTGGCATCC 
GAAGATTATG GCAAAGACCT AGCTTCTGTG AACAACCTGC TGAAAAAGCA TCAACTGCTG 
GAAGCAGATA TATCTGCCCA TGAGGATCGC CTGAAGGACC TGAACAGCCA GGCAGACAGC 
CTGATGACCA GCAGTGCCTT CGACACCTCC CAAGTAAAGG ACAAGAGGGA CACCATCAAC 
GGGCGCTTCC AGAAGATCAA GAGCATGGCG GCCTCCCGGC GAGCCAAGCT GAATGAATCC 
CATCGCCTGC ACCAGTTCTT CCGGGACATG GATGACGAGG AGTCCTGGAT CAAGGAGAAG 
AAGCTGCTGG TGGGCTCAGA GGACTACGGC CGGGACCTAA CCGGCGTGCA GAACCTGAGG 
AAGAAGCACA AGCGGCTGGA AGCAGAACTG GCTGCGCATG AGCCGGCTAT TCAGGGTGTC 
CTGGACACTG GCAAGAAGCT GTCCGATGAC AACACCATCG GGAAAGAGGA GATCCAGCAG 
CGGCTGGCGC AGTTTGTGGA GCACTGGAAA GAGCTGAAGC AGCTGGCAGC TGCCCGGGGT 
CAGCGGCTGG AAGAGTCCTT GGAATATCAG CAGTTTGTAG CCAATGTGGA AGAGGAAGAA 
GCCTGGATCA ATGAGAAAAT GACCCTGGTG GCCAGCGAAG ATTATGGCGA CACTCTTGCC 
GCCATCCAGG GCTTACTGAA GAAACATGAA GCTTTTGAGA CAGACTTCAC CGTCCACAAG 
GATCGCGTGA ATGATGTCTG CACCAATGGA CAAGACCTCA TTAAGAAGAA CAATCACCAT 
GAGGAGAACA TCTCTTCAAA GATGAAGGGC CTGAACGGGA AAGTGTCAGA CCTGGAGAAA 
GCTGCAGCCC AGAGAAAGGC GAAGCTGGAT GAGAACTCGG CCTTCCTTCA GTTCAACTGG 
AAGGCGGACG TGGTGGAGTC CTGGATCGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT 
TATGGCCGAG ACCTGTCTTC TGTGCAGACG CTCCTCACCA AACAGGAAAC TTTTGACGCT 
GGGCTGCAGG CCTTCCAGCA GGAAGGCATT GCCAACATCA CTGCCCTCAA AGATCAGCTT 
CTCGCCGCCA AACACGTTCA GTCCAAGGCC ATCGAGGCCC GGCACGCCTC CCTCATGAAG 
AGGTGGAGCC AGCTTCTGGC CAACTCAGCC GCCCGCAAGA AGAAGCTTCT GGAGGCTCAG 
AGTCACTTCC GCAAGGTGGA GGACCTCTTC CTGACCTTCG CCAAAAAGGC TTCTGCCTTC 
AACAGCTGGT TTGAAAATGC AGAGGAGGAC TTAACAGACC CCGTGCGCTG CAACTCCTTG 
GAAGAAATCA AAGCTTTGCG CGAGGCCCAC GACGCCTTCC GCTCCTCCCT CAGCTCTGCC 
CAGGCTGACT TCAACCAGCT GGCCGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTAGCC 
TCCAACCCCT ACACCTGGTT TACCATGGAG GCCCTGGAGG AGACCTGGAG GAACCTACAG 
AAAATCATCA AGGAGAGGGA GCTGGAGCTG CAGAAGGAAC AGCGGCGGCA GGAGGAGAAC 
GACAAGCTGC GCCAGGAGTT TGCCCAGCAC GCCAACGCCT TCCACCAGTG GATCCAAGAG 
ACCAGGACAT ACCTCCTCGA TGGCATAGCA TATCGTCGGG TCATTCGTGT CTATCAGTAT 
GAAGTTGGGG ATGATCTGTC TGGAAGGTCC TGTATGGTGG AAGAGTCGGG GACCCTCGAA 
TCCCAGCTTG AAGCTACCAA ACGCAAGCAC CAGGAAATCC GAGCCATGAG AAGTCAGCTC 
AAAAAGATCG AGGACCTGGG GGCCGCCATG GAGGAGGCCC TCATCCTGGA CAACAAGTAC 
ACGGAGCACA GCACCGTGGG CCTCGCCCAG CAGTGGGACC AGCTGGACCA GCTGGGCATG 
CGCATGCAGC ACAACCTGGA GCAGCAGATC CAGGCCAGGA ACACAACAGG TGTGACTGAG 
GAGGCCCTCA AAGAATTCAG CATGATGTTT AAACACTTTG ACAAGGACAA GTCTGGCAGG 
CTGAACCATC AGGAGTTCAA ATCTTGCCTG CGCTCCCTGG GCTATGACCT GCCCATGGTG 
GAGGAAGGGG AACCTGACCC TGAGTTCGAG GCAATCCTGG ACACGGTGGA TCCGAACAGA 
GATGGCCATG TCTCCTTGCA AGAATACATG GCTTTCATGA TCAGCCGCGA AACTGAGAAC 
GTCAAGTCCA GCGAGGAGAT TGAGAGCGCC TTCCGGGCCC TCAGCTCAGA GGGAAAGCCT 
TACGTGACCA AGGAGGAGCT CTACCAGAAC CTGACCCGGG AACAAGCCGA CTACTGCGTC 
TCCCACATGA AGCCCTACGT GGACGGCAAG GGCCGCGAGC TCCCCACCGC GTTCGACTAC 
GTGGAGTTCA CCCGCTCGCT TTTCGTGAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006717308.1
CDS	14..7546
Translation	MLSSFRKRRVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELASDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMRRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPTDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRITKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGMMPRDETDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKQKLDILDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAAGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINGRFQKIKSMAASRRAKLNESHRLHQFFRDMDDEESWIKEKKLLVGSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHASLMKRWSQLLANSAARKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGIAYRRVIRVYQYEVGDDLSGRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_006717245.1
Organism	Homo sapiens(human)
Definition	Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006717245.1

ORF Insert Sequence:

ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACCG ATACCACCGC 
TTCAAGGAAC TCTCAACCCT TAGGCGTCAG AAGCTGGAAG ATTCCTATCG ATTCCAGTTC 
TTTCAAAGAG ATGCTGAAGA GCTGGAGAAA TGGATACAGG AAAAACTTCA GATTGCATCT 
GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGAAAGC TTCAGAAGCA TCAAGCATTT 
GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAGC TGGATGAAAC TGGAAACCTG 
ATGATCTCAG AAGGGCATTT TGCATCTGAA ACCATACGGA CCCGTTTGAT GGAGCTGCAC 
CGCCAGTGGG AATTACTTTT GGAGAAGATG CGAGAAAAAG GAATCAAACT GCTGCAGGCC 
CAGAAGTTGG TGCAGTACTT ACGAGAATGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCAATTG TTACTTCTGA AGAGCTGGGC CAGGATCTGG AGCATGTAGA GGTTTTACAG 
AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCTCATG AAGAAAGAGT TAATGAAGTG 
AACCAGTTTG CTGCCAAACT CATACAGGAG CAGCACCCTG AGGAGGAACT GATCAAGACT 
AAGCAGGATG AAGTCAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCTCT GCAGAGGCAG 
GGGAAGCTCT TTGGGGCAGC AGAAGTTCAG CGCTTTAACA GGGATGTGGA TGAGACTATC 
AGTTGGATTA AGGAAAAGGA GCAGTTAATG GCCTCTGATG ATTTTGGCCG AGACCTGGCA 
AGTGTTCAGG CTCTGCTTCG GAAGCACGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAA 
GACAAGGTCA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCCCA CCCTCTGAGT 
GCAACACAGA TTCAAGTGAA GCGAGAGGAA CTGATTACAA ACTGGGAGCA GATCCGCACC 
TTGGCGGCAG AGAGACATGC ACGGCTCAAT GATTCATACA GGCTTCAACG CTTCCTTGCT 
GACTTCCGTG ACCTCACCAG CTGGGTGACT GAGATGAAAG CCCTCATCAA TGCAGATGAG 
CTTGCCAGTG ATGTGGCTGG GGCTGAAGCC CTGCTAGATA GACACCAAGA GCACAAGGGT 
GAAATTGATG CCCATGAAGA CAGCTTCAAA TCTGCAGATG AATCTGGACA GGCACTGCTT 
GCTGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCGTCCT TTCCGAGGAG 
AGAGCGGCGC TGCTGGAGCT GTGGGAGCTG CGCAGGCAGC AGTACGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGGGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ATGAAGACTT GGGAGATTCC TTGGATAGTG TGGAAGCGCT TCTTAAGAAG 
CACGAAGACT TTGAGAAATC CCTTAGTGCC CAGGAGGAAA AGATTACAGC ATTAGATGAA 
TTTGCAACCA AGCTAATTCA GAACAACCAC TATGCAATGG AAGATGTGGC CACTCGCCGA 
GATGCTCTGT TGAGCCGCCG CAATGCCCTT CACGAGAGAG CCATGCGTCG CCGGGCCCAG 
CTAGCCGATT CTTTCCATCT GCAGCAGTTT TTCCGTGATT CTGATGAGCT CAAGAGTTGG 
GTCAATGAGA AGATGAAAAC TGCCACAGAT GAAGCTTATA AAGATCCATC CAACCTACAA 
GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CAGCAAACCA GAGCCGAATT 
GATGCCTTGG AGAAAGCTGG CCAAAAGCTG ATTGATGTCA ACCACTATGC CAAGGATGAA 
GTGGCAGCTC GTATGAATGA GGTGATCAGT TTGTGGAAGA AACTGCTAGA GGCCACTGAA 
CTGAAAGGAA TAAAGCTTCG TGAAGCCAAC CAGCAACAGC AATTTAATCG CAATGTTGAG 
GATATTGAAT TGTGGCTATA TGAAGTAGAA GGTCACTTGG CTTCGGATGA TTACGGCAAA 
GATCTTACCA ATGTGCAGAA CCTCCAGAAG AAACATGCAC TGCTAGAGGC AGATGTGGCT 
GCTCACCAGG ACCGAATTGA TGGCATCACC ATTCAGGCCC GCCAGTTCCA AGATGCTGGC 
CATTTTGATG CAGAAAACAT CAAGAAGAAA CAGGAAGCCC TCGTGGCTCG CTATGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCTCTGCG GTTGCAGCAG 
CTCTTCCGGG ATGTTGAGGA TGAGGAGACG TGGATTCGAG AGAAAGAGCC CATTGCCGCA 
TCTACCAACA GAGGTAAGGA TTTAATTGGG GTCCAGAATC TGCTAAAGAA ACATCAAGCC 
TTACAAGCAG AAATTGCTGG ACATGAACCA CGCATCAAAG CAGTTACACA GAAGGGGAAT 
GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAGCT TCACGAGCTG 
AACCAAAAGT GGGAGGCACT GAAAGCCAAA GCTTCCCAGC GTCGGCAGGA CCTGGAGGAC 
TCTCTGCAGG CCCAGCAGTA CTTTGCTGAT GCTAACGAGG CTGAATCCTG GATGCGGGAG 
AAGGAACCCA TTGTGGGCAG CACTGACTAT GGCAAGGACG AAGACTCTGC TGAGGCTCTA 
CTGAAGAAAC ACGAAGCTTT GATGTCAGAT CTCAGTGCCT ACGGCAGCAG CATCCAGGCT 
TTGCGAGAAC AAGCACAGTC CTGCCGGCAA CAAGTGGCCC CCACGGATGA TGAGACTGGG 
AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAGGGAG ATATCCTTAC CTTACTCAAC AGCACCAACA AGGATTGGTG GAAAGTGGAA 
GTGAACGATC GTCAGGGTTT TGTGCCGGCT GCGTACGTGA AGAAATTGGA CCCCGCCCAG 
TCAGCCTCCC GGGAGAATCT CCTGGAGGAG CAAGGCAGCA TAGCACTGCG GCAGGAGCAG 
ATTGACAATC AGACACGCAT AACTAAGGAG GCCGGCAGTG TATCTCTGCG TATGAAGCAG 
GTGGAAGAAC TATATCATTC TCTGCTGGAA CTGGGTGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGTTGCA AGAAGTTTAT GTTGTTCCGT GAAGCGAATG AACTACAGCA ATGGATCAAT 
GAGAAGGAAG CCGCTCTGAC AAGTGAGGAG GTCGGAGCAG ACTTGGAGCA GGTTGAGGTG 
CTCCAGAAGA AGTTTGATGA CTTCCAGAAG GACCTGAAGG CCAATGAGTC ACGGTTGAAG 
GACATTAACA AGGTAGCTGA AGACCTGGAG TCTGAAGGTC TCATGGCAGA GGAGGTGCAG 
GCTGTGCAAC AACAGGAAGT GTATGGCATG ATGCCCAGGG ATGAAACTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGTCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTGA ATGAGCGCTG GCGGTCCCTA CAGCAGCTGG CCGAGGAACG GAGCCAGCTC 
TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CTGATGAAAC CAAAGAATGG 
ATTGAAGAGA AGAATCAAGC TCTAAACACA GACAATTATG GACATGATCT CGCCAGTGTC 
CAGGCCCTGC AACGCAAGCA TGAGGGCTTC GAGAGGGACC TTGCGGCTCT CGGTGACAAG 
GTAAACTCCC TTGGTGAAAC AGCAGAGCGC CTGATCCAGT CCCATCCCGA GTCAGCAGAA 
GACCTGCAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA GCAGCCTGGG GAAACGTGCA 
GATCAGCGCA AGGCAAAGTT GGGTGACTCC CACGACCTGC AGCGCTTCCT TAGCGATTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATA CGGGGGTTGG TGTCCTCAGA TGAGCTAGCC 
AAGGATGTCA CCGGAGCTGA GGCATTGCTG GAGCGACACC AGGAACACCG GACAGAAATC 
GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GACAGCAGCT GTTGGCTCAC 
GGACACTATG CCAGCCCTGA GATCAAGCAG AAACTTGATA TTCTTGACCA GGAGCGTGCA 
GACCTGGAGA AGGCCTGGGT TCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAACTGCAG 
CTGTTCCATC GGGACTGTGA GCAAGCTGAG AACTGGATGG CTGCCCGGGA GGCCTTCTTG 
AATACCGAAG ACAAAGGAGA CTCACTGGAC AGCGTAGAGG CTCTGATCAA AAAACATGAA 
GACTTTGACA AAGCGATTAA CGTCCAGGAA GAGAAGATTG CTGCTCTGCA GGCCTTTGCC 
GACCAGCTCA TCGCTGCCGG CCATTATGCC AAGGGAGACA TTTCTAGCCG GCGCAATGAG 
GTCTTGGACA GGTGGCGACG TCTGAAAGCC CAGATGATTG AGAAAAGGTC AAAGCTAGGA 
GAATCTCAAA CCCTCCAACA GTTCAGCCGG GATGTGGATG AGATTGAGGC TTGGATCAGT 
GAAAAATTGC AAACAGCGAG TGATGAGTCG TACAAGGATC CCACCAACAT CCAGCTTTCC 
AAGCTGCTGA GCAAGCACCA GAAGCACCAG GCTTTTGAAG CAGAGCTGCA TGCCAACGCT 
GACCGGATCC GTGGGGTTAT CGACATGGGC AACTCCCTCA TTGAACGTGG AGCCTGTGCC 
GGCAGTGAGG ATGCTGTCAA GGCCCGCCTG GCTGCCTTAG CTGACCAGTG GCAGTTCTTG 
GTGCAAAAGT CAGCGGAAAA GAGCCAGAAA CTGAAAGAAG CCAACAAGCA GCAGAACTTC 
AACACAGGGA TCAAGGACTT TGACTTCTGG CTGTCTGAGG TGGAGGCCCT GCTGGCATCC 
GAAGATTATG GCAAAGACCT AGCTTCTGTG AACAACCTGC TGAAAAAGCA TCAACTGCTG 
GAAGCAGATA TATCTGCCCA TGAGGATCGC CTGAAGGACC TGAACAGCCA GGCAGACAGC 
CTGATGACCA GCAGTGCCTT CGACACCTCC CAAGTAAAGG ACAAGAGGGA CACCATCAAC 
GGGCGCTTCC AGAAGATCAA GAGCATGGCG GCCTCCCGGC GAGCCAAGCT GAATGAATCC 
CATCGCCTGC ACCAGTTCTT CCGGGACATG GATGACGAGG AGTCCTGGAT CAAGGAGAAG 
AAGCTGCTGG TGGGCTCAGA GGACTACGGC CGGGACCTAA CCGGCGTGCA GAACCTGAGG 
AAGAAGCACA AGCGGCTGGA AGCAGAACTG GCTGCGCATG AGCCGGCTAT TCAGGGTGTC 
CTGGACACTG GCAAGAAGCT GTCCGATGAC AACACCATCG GGAAAGAGGA GATCCAGCAG 
CGGCTGGCGC AGTTTGTGGA GCACTGGAAA GAGCTGAAGC AGCTGGCAGC TGCCCGGGGT 
CAGCGGCTGG AAGAGTCCTT GGAATATCAG CAGTTTGTAG CCAATGTGGA AGAGGAAGAA 
GCCTGGATCA ATGAGAAAAT GACCCTGGTG GCCAGCGAAG ATTATGGCGA CACTCTTGCC 
GCCATCCAGG GCTTACTGAA GAAACATGAA GCTTTTGAGA CAGACTTCAC CGTCCACAAG 
GATCGCGTGA ATGATGTCTG CACCAATGGA CAAGACCTCA TTAAGAAGAA CAATCACCAT 
GAGGAGAACA TCTCTTCAAA GATGAAGGGC CTGAACGGGA AAGTGTCAGA CCTGGAGAAA 
GCTGCAGCCC AGAGAAAGGC GAAGCTGGAT GAGAACTCGG CCTTCCTTCA GTTCAACTGG 
AAGGCGGACG TGGTGGAGTC CTGGATCGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT 
TATGGCCGAG ACCTGTCTTC TGTGCAGACG CTCCTCACCA AACAGGAAAC TTTTGACGCT 
GGGCTGCAGG CCTTCCAGCA GGAAGGCATT GCCAACATCA CTGCCCTCAA AGATCAGCTT 
CTCGCCGCCA AACACGTTCA GTCCAAGGCC ATCGAGGCCC GGCACGCCTC CCTCATGAAG 
AGGTGGAGCC AGCTTCTGGC CAACTCAGCC GCCCGCAAGA AGAAGCTTCT GGAGGCTCAG 
AGTCACTTCC GCAAGGTGGA GGACCTCTTC CTGACCTTCG CCAAAAAGGC TTCTGCCTTC 
AACAGCTGGT TTGAAAATGC AGAGGAGGAC TTAACAGACC CCGTGCGCTG CAACTCCTTG 
GAAGAAATCA AAGCTTTGCG CGAGGCCCAC GACGCCTTCC GCTCCTCCCT CAGCTCTGCC 
CAGGCTGACT TCAACCAGCT GGCCGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTAGCC 
TCCAACCCCT ACACCTGGTT TACCATGGAG GCCCTGGAGG AGACCTGGAG GAACCTACAG 
AAAATCATCA AGGAGAGGGA GCTGGAGCTG CAGAAGGAAC AGCGGCGGCA GGAGGAGAAC 
GACAAGCTGC GCCAGGAGTT TGCCCAGCAC GCCAACGCCT TCCACCAGTG GATCCAAGAG 
ACCAGGACAT ACCTCCTCGA TGGCATAGCA TATCGTCGGG TCATTCGTGT CTATCAGTAT 
GAAGTTGGGG ATGATCTGTC TGGAAGGTCC TGTATGGTGG AAGAGTCGGG GACCCTCGAA 
TCCCAGCTTG AAGCTACCAA ACGCAAGCAC CAGGAAATCC GAGCCATGAG AAGTCAGCTC 
AAAAAGATCG AGGACCTGGG GGCCGCCATG GAGGAGGCCC TCATCCTGGA CAACAAGTAC 
ACGGAGCACA GCACCGTGGG CCTCGCCCAG CAGTGGGACC AGCTGGACCA GCTGGGCATG 
CGCATGCAGC ACAACCTGGA GCAGCAGATC CAGGCCAGGA ACACAACAGG TGTGACTGAG 
GAGGCCCTCA AAGAATTCAG CATGATGTTT AAACACTTTG ACAAGGACAA GTCTGGCAGG 
CTGAACCATC AGGAGTTCAA ATCTTGCCTG CGCTCCCTGG GCTATGACCT GCCCATGGTG 
GAGGAAGGGG AACCTGACCC TGAGTTCGAG GCAATCCTGG ACACGGTGGA TCCGAACAGA 
GATGGCCATG TCTCCTTGCA AGAATACATG GCTTTCATGA TCAGCCGCGA AACTGAGAAC 
GTCAAGTCCA GCGAGGAGAT TGAGAGCGCC TTCCGGGCCC TCAGCTCAGA GGGAAAGCCT 
TACGTGACCA AGGAGGAGCT CTACCAGAAC CTGACCCGGG AACAAGCCGA CTACTGCGTC 
TCCCACATGA AGCCCTACGT GGACGGCAAG GGCCGCGAGC TCCCCACCGC GTTCGACTAC 
GTGGAGTTCA CCCGCTCGCT TTTCGTGAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_006717246.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_006717309.1 Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X2, mRNA.

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CloneID	OHu43454
Clone ID Related Accession (Same CDS sequence)	XM_006717246.1
Accession Version	XM_006717246.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7518bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACCG ATACCACCGC 
TTCAAGGAAC TCTCAACCCT TAGGCGTCAG AAGCTGGAAG ATTCCTATCG ATTCCAGTTC 
TTTCAAAGAG ATGCTGAAGA GCTGGAGAAA TGGATACAGG AAAAACTTCA GATTGCATCT 
GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGAAAGC TTCAGAAGCA TCAAGCATTT 
GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAGC TGGATGAAAC TGGAAACCTG 
ATGATCTCAG AAGGGCATTT TGCATCTGAA ACCATACGGA CCCGTTTGAT GGAGCTGCAC 
CGCCAGTGGG AATTACTTTT GGAGAAGATG CGAGAAAAAG GAATCAAACT GCTGCAGGCC 
CAGAAGTTGG TGCAGTACTT ACGAGAATGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCAATTG TTACTTCTGA AGAGCTGGGC CAGGATCTGG AGCATGTAGA GGTTTTACAG 
AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCTCATG AAGAAAGAGT TAATGAAGTG 
AACCAGTTTG CTGCCAAACT CATACAGGAG CAGCACCCTG AGGAGGAACT GATCAAGACT 
AAGCAGGATG AAGTCAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCTCT GCAGAGGCAG 
GGGAAGCTCT TTGGGGCAGC AGAAGTTCAG CGCTTTAACA GGGATGTGGA TGAGACTATC 
AGTTGGATTA AGGAAAAGGA GCAGTTAATG GCCTCTGATG ATTTTGGCCG AGACCTGGCA 
AGTGTTCAGG CTCTGCTTCG GAAGCACGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAA 
GACAAGGTCA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCCCA CCCTCTGAGT 
GCAACACAGA TTCAAGTGAA GCGAGAGGAA CTGATTACAA ACTGGGAGCA GATCCGCACC 
TTGGCGGCAG AGAGACATGC ACGGCTCAAT GATTCATACA GGCTTCAACG CTTCCTTGCT 
GACTTCCGTG ACCTCACCAG CTGGGTGACT GAGATGAAAG CCCTCATCAA TGCAGATGAG 
CTTGCCAGTG ATGTGGCTGG GGCTGAAGCC CTGCTAGATA GACACCAAGA GCACAAGGGT 
GAAATTGATG CCCATGAAGA CAGCTTCAAA TCTGCAGATG AATCTGGACA GGCACTGCTT 
GCTGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCGTCCT TTCCGAGGAG 
AGAGCGGCGC TGCTGGAGCT GTGGGAGCTG CGCAGGCAGC AGTACGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGGGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ATGAAGACTT GGGAGATTCC TTGGATAGTG TGGAAGCGCT TCTTAAGAAG 
CACGAAGACT TTGAGAAATC CCTTAGTGCC CAGGAGGAAA AGATTACAGC ATTAGATGAA 
TTTGCAACCA AGCTAATTCA GAACAACCAC TATGCAATGG AAGATGTGGC CACTCGCCGA 
GATGCTCTGT TGAGCCGCCG CAATGCCCTT CACGAGAGAG CCATGCGTCG CCGGGCCCAG 
CTAGCCGATT CTTTCCATCT GCAGCAGTTT TTCCGTGATT CTGATGAGCT CAAGAGTTGG 
GTCAATGAGA AGATGAAAAC TGCCACAGAT GAAGCTTATA AAGATCCATC CAACCTACAA 
GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CAGCAAACCA GAGCCGAATT 
GATGCCTTGG AGAAAGCTGG CCAAAAGCTG ATTGATGTCA ACCACTATGC CAAGGATGAA 
GTGGCAGCTC GTATGAATGA GGTGATCAGT TTGTGGAAGA AACTGCTAGA GGCCACTGAA 
CTGAAAGGAA TAAAGCTTCG TGAAGCCAAC CAGCAACAGC AATTTAATCG CAATGTTGAG 
GATATTGAAT TGTGGCTATA TGAAGTAGAA GGTCACTTGG CTTCGGATGA TTACGGCAAA 
GATCTTACCA ATGTGCAGAA CCTCCAGAAG AAACATGCAC TGCTAGAGGC AGATGTGGCT 
GCTCACCAGG ACCGAATTGA TGGCATCACC ATTCAGGCCC GCCAGTTCCA AGATGCTGGC 
CATTTTGATG CAGAAAACAT CAAGAAGAAA CAGGAAGCCC TCGTGGCTCG CTATGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCTCTGCG GTTGCAGCAG 
CTCTTCCGGG ATGTTGAGGA TGAGGAGACG TGGATTCGAG AGAAAGAGCC CATTGCCGCA 
TCTACCAACA GAGGTAAGGA TTTAATTGGG GTCCAGAATC TGCTAAAGAA ACATCAAGCC 
TTACAAGCAG AAATTGCTGG ACATGAACCA CGCATCAAAG CAGTTACACA GAAGGGGAAT 
GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAGCT TCACGAGCTG 
AACCAAAAGT GGGAGGCACT GAAAGCCAAA GCTTCCCAGC GTCGGCAGGA CCTGGAGGAC 
TCTCTGCAGG CCCAGCAGTA CTTTGCTGAT GCTAACGAGG CTGAATCCTG GATGCGGGAG 
AAGGAACCCA TTGTGGGCAG CACTGACTAT GGCAAGGACG AAGACTCTGC TGAGGCTCTA 
CTGAAGAAAC ACGAAGCTTT GATGTCAGAT CTCAGTGCCT ACGGCAGCAG CATCCAGGCT 
TTGCGAGAAC AAGCACAGTC CTGCCGGCAA CAAGTGGCCC CCACGGATGA TGAGACTGGG 
AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAGGGAG ATATCCTTAC CTTACTCAAC AGCACCAACA AGGATTGGTG GAAAGTGGAA 
GTGAACGATC GTCAGGGTTT TGTGCCGGCT GCGTACGTGA AGAAATTGGA CCCCGCCCAG 
TCAGCCTCCC GGGAGAATCT CCTGGAGGAG CAAGGCAGCA TAGCACTGCG GCAGGAGCAG 
ATTGACAATC AGACACGCAT AACTAAGGAG GCCGGCAGTG TATCTCTGCG TATGAAGCAG 
GTGGAAGAAC TATATCATTC TCTGCTGGAA CTGGGTGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGTTGCA AGAAGTTTAT GTTGTTCCGT GAAGCGAATG AACTACAGCA ATGGATCAAT 
GAGAAGGAAG CCGCTCTGAC AAGTGAGGAG GTCGGAGCAG ACTTGGAGCA GGTTGAGGTG 
CTCCAGAAGA AGTTTGATGA CTTCCAGAAG GACCTGAAGG CCAATGAGTC ACGGTTGAAG 
GACATTAACA AGGTAGCTGA AGACCTGGAG TCTGAAGGTC TCATGGCAGA GGAGGTGCAG 
GCTGTGCAAC AACAGGAAGT GTATGGCATG ATGCCCAGGG ATGAAACTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGTCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTGA ATGAGCGCTG GCGGTCCCTA CAGCAGCTGG CCGAGGAACG GAGCCAGCTC 
TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CTGATGAAAC CAAAGAATGG 
ATTGAAGAGA AGAATCAAGC TCTAAACACA GACAATTATG GACATGATCT CGCCAGTGTC 
CAGGCCCTGC AACGCAAGCA TGAGGGCTTC GAGAGGGACC TTGCGGCTCT CGGTGACAAG 
GTAAACTCCC TTGGTGAAAC AGCAGAGCGC CTGATCCAGT CCCATCCCGA GTCAGCAGAA 
GACCTGCAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA GCAGCCTGGG GAAACGTGCA 
GATCAGCGCA AGGCAAAGTT GGGTGACTCC CACGACCTGC AGCGCTTCCT TAGCGATTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATA CGGGGGTTGG TGTCCTCAGA TGAGCTAGCC 
AAGGATGTCA CCGGAGCTGA GGCATTGCTG GAGCGACACC AGGAACACCG GACAGAAATC 
GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GACAGCAGCT GTTGGCTCAC 
GGACACTATG CCAGCCCTGA GATCAAGCAG AAACTTGATA TTCTTGACCA GGAGCGTGCA 
GACCTGGAGA AGGCCTGGGT TCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAACTGCAG 
CTGTTCCATC GGGACTGTGA GCAAGCTGAG AACTGGATGG CTGCCCGGGA GGCCTTCTTG 
AATACCGAAG ACAAAGGAGA CTCACTGGAC AGCGTAGAGG CTCTGATCAA AAAACATGAA 
GACTTTGACA AAGCGATTAA CGTCCAGGAA GAGAAGATTG CTGCTCTGCA GGCCTTTGCC 
GACCAGCTCA TCGCTGCCGG CCATTATGCC AAGGGAGACA TTTCTAGCCG GCGCAATGAG 
GTCTTGGACA GGTGGCGACG TCTGAAAGCC CAGATGATTG AGAAAAGGTC AAAGCTAGGA 
GAATCTCAAA CCCTCCAACA GTTCAGCCGG GATGTGGATG AGATTGAGGC TTGGATCAGT 
GAAAAATTGC AAACAGCGAG TGATGAGTCG TACAAGGATC CCACCAACAT CCAGAGCAAG 
CACCAGAAGC ACCAGGCTTT TGAAGCAGAG CTGCATGCCA ACGCTGACCG GATCCGTGGG 
GTTATCGACA TGGGCAACTC CCTCATTGAA CGTGGAGCCT GTGCCGGCAG TGAGGATGCT 
GTCAAGGCCC GCCTGGCTGC CTTAGCTGAC CAGTGGCAGT TCTTGGTGCA AAAGTCAGCG 
GAAAAGAGCC AGAAACTGAA AGAAGCCAAC AAGCAGCAGA ACTTCAACAC AGGGATCAAG 
GACTTTGACT TCTGGCTGTC TGAGGTGGAG GCCCTGCTGG CATCCGAAGA TTATGGCAAA 
GACCTAGCTT CTGTGAACAA CCTGCTGAAA AAGCATCAAC TGCTGGAAGC AGATATATCT 
GCCCATGAGG ATCGCCTGAA GGACCTGAAC AGCCAGGCAG ACAGCCTGAT GACCAGCAGT 
GCCTTCGACA CCTCCCAAGT AAAGGACAAG AGGGACACCA TCAACGGGCG CTTCCAGAAG 
ATCAAGAGCA TGGCGGCCTC CCGGCGAGCC AAGCTGAATG AATCCCATCG CCTGCACCAG 
TTCTTCCGGG ACATGGATGA CGAGGAGTCC TGGATCAAGG AGAAGAAGCT GCTGGTGGGC 
TCAGAGGACT ACGGCCGGGA CCTAACCGGC GTGCAGAACC TGAGGAAGAA GCACAAGCGG 
CTGGAAGCAG AACTGGCTGC GCATGAGCCG GCTATTCAGG GTGTCCTGGA CACTGGCAAG 
AAGCTGTCCG ATGACAACAC CATCGGGAAA GAGGAGATCC AGCAGCGGCT GGCGCAGTTT 
GTGGAGCACT GGAAAGAGCT GAAGCAGCTG GCAGCTGCCC GGGGTCAGCG GCTGGAAGAG 
TCCTTGGAAT ATCAGCAGTT TGTAGCCAAT GTGGAAGAGG AAGAAGCCTG GATCAATGAG 
AAAATGACCC TGGTGGCCAG CGAAGATTAT GGCGACACTC TTGCCGCCAT CCAGGGCTTA 
CTGAAGAAAC ATGAAGCTTT TGAGACAGAC TTCACCGTCC ACAAGGATCG CGTGAATGAT 
GTCTGCACCA ATGGACAAGA CCTCATTAAG AAGAACAATC ACCATGAGGA GAACATCTCT 
TCAAAGATGA AGGGCCTGAA CGGGAAAGTG TCAGACCTGG AGAAAGCTGC AGCCCAGAGA 
AAGGCGAAGC TGGATGAGAA CTCGGCCTTC CTTCAGTTCA ACTGGAAGGC GGACGTGGTG 
GAGTCCTGGA TCGGTGAAAA GGAGAACAGC TTGAAGACAG ATGATTATGG CCGAGACCTG 
TCTTCTGTGC AGACGCTCCT CACCAAACAG GAAACTTTTG ACGCTGGGCT GCAGGCCTTC 
CAGCAGGAAG GCATTGCCAA CATCACTGCC CTCAAAGATC AGCTTCTCGC CGCCAAACAC 
GTTCAGTCCA AGGCCATCGA GGCCCGGCAC GCCTCCCTCA TGAAGAGGTG GAGCCAGCTT 
CTGGCCAACT CAGCCGCCCG CAAGAAGAAG CTTCTGGAGG CTCAGAGTCA CTTCCGCAAG 
GTGGAGGACC TCTTCCTGAC CTTCGCCAAA AAGGCTTCTG CCTTCAACAG CTGGTTTGAA 
AATGCAGAGG AGGACTTAAC AGACCCCGTG CGCTGCAACT CCTTGGAAGA AATCAAAGCT 
TTGCGCGAGG CCCACGACGC CTTCCGCTCC TCCCTCAGCT CTGCCCAGGC TGACTTCAAC 
CAGCTGGCCG AGCTGGACCG CCAGATCAAG AGCTTCCGCG TAGCCTCCAA CCCCTACACC 
TGGTTTACCA TGGAGGCCCT GGAGGAGACC TGGAGGAACC TACAGAAAAT CATCAAGGAG 
AGGGAGCTGG AGCTGCAGAA GGAACAGCGG CGGCAGGAGG AGAACGACAA GCTGCGCCAG 
GAGTTTGCCC AGCACGCCAA CGCCTTCCAC CAGTGGATCC AAGAGACCAG GACATACCTC 
CTCGATGGCA TAGCATATCG TCGGGTCATT CGTGTCTATC AGTATGAAGT TGGGGATGAT 
CTGTCTGGAA GGTCCTGTAT GGTGGAAGAG TCGGGGACCC TCGAATCCCA GCTTGAAGCT 
ACCAAACGCA AGCACCAGGA AATCCGAGCC ATGAGAAGTC AGCTCAAAAA GATCGAGGAC 
CTGGGGGCCG CCATGGAGGA GGCCCTCATC CTGGACAACA AGTACACGGA GCACAGCACC 
GTGGGCCTCG CCCAGCAGTG GGACCAGCTG GACCAGCTGG GCATGCGCAT GCAGCACAAC 
CTGGAGCAGC AGATCCAGGC CAGGAACACA ACAGGTGTGA CTGAGGAGGC CCTCAAAGAA 
TTCAGCATGA TGTTTAAACA CTTTGACAAG GACAAGTCTG GCAGGCTGAA CCATCAGGAG 
TTCAAATCTT GCCTGCGCTC CCTGGGCTAT GACCTGCCCA TGGTGGAGGA AGGGGAACCT 
GACCCTGAGT TCGAGGCAAT CCTGGACACG GTGGATCCGA ACAGAGATGG CCATGTCTCC 
TTGCAAGAAT ACATGGCTTT CATGATCAGC CGCGAAACTG AGAACGTCAA GTCCAGCGAG 
GAGATTGAGA GCGCCTTCCG GGCCCTCAGC TCAGAGGGAA AGCCTTACGT GACCAAGGAG 
GAGCTCTACC AGAACCTGAC CCGGGAACAA GCCGACTACT GCGTCTCCCA CATGAAGCCC 
TACGTGGACG GCAAGGGCCG CGAGCTCCCC ACCGCGTTCG ACTACGTGGA GTTCACCCGC 
TCGCTTTTCG TGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006717309.1
CDS	14..7531
Translation	MLSSFRKRRVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELASDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMRRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPTDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRITKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGMMPRDETDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKQKLDILDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAAGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINGRFQKIKSMAASRRAKLNESHRLHQFFRDMDDEESWIKEKKLLVGSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHASLMKRWSQLLANSAARKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGIAYRRVIRVYQYEVGDDLSGRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_006717246.1
Organism	Homo sapiens(human)
Definition	Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006717246.1

ORF Insert Sequence:

ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACCG ATACCACCGC 
TTCAAGGAAC TCTCAACCCT TAGGCGTCAG AAGCTGGAAG ATTCCTATCG ATTCCAGTTC 
TTTCAAAGAG ATGCTGAAGA GCTGGAGAAA TGGATACAGG AAAAACTTCA GATTGCATCT 
GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGAAAGC TTCAGAAGCA TCAAGCATTT 
GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAGC TGGATGAAAC TGGAAACCTG 
ATGATCTCAG AAGGGCATTT TGCATCTGAA ACCATACGGA CCCGTTTGAT GGAGCTGCAC 
CGCCAGTGGG AATTACTTTT GGAGAAGATG CGAGAAAAAG GAATCAAACT GCTGCAGGCC 
CAGAAGTTGG TGCAGTACTT ACGAGAATGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCAATTG TTACTTCTGA AGAGCTGGGC CAGGATCTGG AGCATGTAGA GGTTTTACAG 
AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCTCATG AAGAAAGAGT TAATGAAGTG 
AACCAGTTTG CTGCCAAACT CATACAGGAG CAGCACCCTG AGGAGGAACT GATCAAGACT 
AAGCAGGATG AAGTCAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCTCT GCAGAGGCAG 
GGGAAGCTCT TTGGGGCAGC AGAAGTTCAG CGCTTTAACA GGGATGTGGA TGAGACTATC 
AGTTGGATTA AGGAAAAGGA GCAGTTAATG GCCTCTGATG ATTTTGGCCG AGACCTGGCA 
AGTGTTCAGG CTCTGCTTCG GAAGCACGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAA 
GACAAGGTCA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCCCA CCCTCTGAGT 
GCAACACAGA TTCAAGTGAA GCGAGAGGAA CTGATTACAA ACTGGGAGCA GATCCGCACC 
TTGGCGGCAG AGAGACATGC ACGGCTCAAT GATTCATACA GGCTTCAACG CTTCCTTGCT 
GACTTCCGTG ACCTCACCAG CTGGGTGACT GAGATGAAAG CCCTCATCAA TGCAGATGAG 
CTTGCCAGTG ATGTGGCTGG GGCTGAAGCC CTGCTAGATA GACACCAAGA GCACAAGGGT 
GAAATTGATG CCCATGAAGA CAGCTTCAAA TCTGCAGATG AATCTGGACA GGCACTGCTT 
GCTGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCGTCCT TTCCGAGGAG 
AGAGCGGCGC TGCTGGAGCT GTGGGAGCTG CGCAGGCAGC AGTACGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGGGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ATGAAGACTT GGGAGATTCC TTGGATAGTG TGGAAGCGCT TCTTAAGAAG 
CACGAAGACT TTGAGAAATC CCTTAGTGCC CAGGAGGAAA AGATTACAGC ATTAGATGAA 
TTTGCAACCA AGCTAATTCA GAACAACCAC TATGCAATGG AAGATGTGGC CACTCGCCGA 
GATGCTCTGT TGAGCCGCCG CAATGCCCTT CACGAGAGAG CCATGCGTCG CCGGGCCCAG 
CTAGCCGATT CTTTCCATCT GCAGCAGTTT TTCCGTGATT CTGATGAGCT CAAGAGTTGG 
GTCAATGAGA AGATGAAAAC TGCCACAGAT GAAGCTTATA AAGATCCATC CAACCTACAA 
GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CAGCAAACCA GAGCCGAATT 
GATGCCTTGG AGAAAGCTGG CCAAAAGCTG ATTGATGTCA ACCACTATGC CAAGGATGAA 
GTGGCAGCTC GTATGAATGA GGTGATCAGT TTGTGGAAGA AACTGCTAGA GGCCACTGAA 
CTGAAAGGAA TAAAGCTTCG TGAAGCCAAC CAGCAACAGC AATTTAATCG CAATGTTGAG 
GATATTGAAT TGTGGCTATA TGAAGTAGAA GGTCACTTGG CTTCGGATGA TTACGGCAAA 
GATCTTACCA ATGTGCAGAA CCTCCAGAAG AAACATGCAC TGCTAGAGGC AGATGTGGCT 
GCTCACCAGG ACCGAATTGA TGGCATCACC ATTCAGGCCC GCCAGTTCCA AGATGCTGGC 
CATTTTGATG CAGAAAACAT CAAGAAGAAA CAGGAAGCCC TCGTGGCTCG CTATGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCTCTGCG GTTGCAGCAG 
CTCTTCCGGG ATGTTGAGGA TGAGGAGACG TGGATTCGAG AGAAAGAGCC CATTGCCGCA 
TCTACCAACA GAGGTAAGGA TTTAATTGGG GTCCAGAATC TGCTAAAGAA ACATCAAGCC 
TTACAAGCAG AAATTGCTGG ACATGAACCA CGCATCAAAG CAGTTACACA GAAGGGGAAT 
GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAGCT TCACGAGCTG 
AACCAAAAGT GGGAGGCACT GAAAGCCAAA GCTTCCCAGC GTCGGCAGGA CCTGGAGGAC 
TCTCTGCAGG CCCAGCAGTA CTTTGCTGAT GCTAACGAGG CTGAATCCTG GATGCGGGAG 
AAGGAACCCA TTGTGGGCAG CACTGACTAT GGCAAGGACG AAGACTCTGC TGAGGCTCTA 
CTGAAGAAAC ACGAAGCTTT GATGTCAGAT CTCAGTGCCT ACGGCAGCAG CATCCAGGCT 
TTGCGAGAAC AAGCACAGTC CTGCCGGCAA CAAGTGGCCC CCACGGATGA TGAGACTGGG 
AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAGGGAG ATATCCTTAC CTTACTCAAC AGCACCAACA AGGATTGGTG GAAAGTGGAA 
GTGAACGATC GTCAGGGTTT TGTGCCGGCT GCGTACGTGA AGAAATTGGA CCCCGCCCAG 
TCAGCCTCCC GGGAGAATCT CCTGGAGGAG CAAGGCAGCA TAGCACTGCG GCAGGAGCAG 
ATTGACAATC AGACACGCAT AACTAAGGAG GCCGGCAGTG TATCTCTGCG TATGAAGCAG 
GTGGAAGAAC TATATCATTC TCTGCTGGAA CTGGGTGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGTTGCA AGAAGTTTAT GTTGTTCCGT GAAGCGAATG AACTACAGCA ATGGATCAAT 
GAGAAGGAAG CCGCTCTGAC AAGTGAGGAG GTCGGAGCAG ACTTGGAGCA GGTTGAGGTG 
CTCCAGAAGA AGTTTGATGA CTTCCAGAAG GACCTGAAGG CCAATGAGTC ACGGTTGAAG 
GACATTAACA AGGTAGCTGA AGACCTGGAG TCTGAAGGTC TCATGGCAGA GGAGGTGCAG 
GCTGTGCAAC AACAGGAAGT GTATGGCATG ATGCCCAGGG ATGAAACTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGTCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTGA ATGAGCGCTG GCGGTCCCTA CAGCAGCTGG CCGAGGAACG GAGCCAGCTC 
TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CTGATGAAAC CAAAGAATGG 
ATTGAAGAGA AGAATCAAGC TCTAAACACA GACAATTATG GACATGATCT CGCCAGTGTC 
CAGGCCCTGC AACGCAAGCA TGAGGGCTTC GAGAGGGACC TTGCGGCTCT CGGTGACAAG 
GTAAACTCCC TTGGTGAAAC AGCAGAGCGC CTGATCCAGT CCCATCCCGA GTCAGCAGAA 
GACCTGCAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA GCAGCCTGGG GAAACGTGCA 
GATCAGCGCA AGGCAAAGTT GGGTGACTCC CACGACCTGC AGCGCTTCCT TAGCGATTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATA CGGGGGTTGG TGTCCTCAGA TGAGCTAGCC 
AAGGATGTCA CCGGAGCTGA GGCATTGCTG GAGCGACACC AGGAACACCG GACAGAAATC 
GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GACAGCAGCT GTTGGCTCAC 
GGACACTATG CCAGCCCTGA GATCAAGCAG AAACTTGATA TTCTTGACCA GGAGCGTGCA 
GACCTGGAGA AGGCCTGGGT TCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAACTGCAG 
CTGTTCCATC GGGACTGTGA GCAAGCTGAG AACTGGATGG CTGCCCGGGA GGCCTTCTTG 
AATACCGAAG ACAAAGGAGA CTCACTGGAC AGCGTAGAGG CTCTGATCAA AAAACATGAA 
GACTTTGACA AAGCGATTAA CGTCCAGGAA GAGAAGATTG CTGCTCTGCA GGCCTTTGCC 
GACCAGCTCA TCGCTGCCGG CCATTATGCC AAGGGAGACA TTTCTAGCCG GCGCAATGAG 
GTCTTGGACA GGTGGCGACG TCTGAAAGCC CAGATGATTG AGAAAAGGTC AAAGCTAGGA 
GAATCTCAAA CCCTCCAACA GTTCAGCCGG GATGTGGATG AGATTGAGGC TTGGATCAGT 
GAAAAATTGC AAACAGCGAG TGATGAGTCG TACAAGGATC CCACCAACAT CCAGAGCAAG 
CACCAGAAGC ACCAGGCTTT TGAAGCAGAG CTGCATGCCA ACGCTGACCG GATCCGTGGG 
GTTATCGACA TGGGCAACTC CCTCATTGAA CGTGGAGCCT GTGCCGGCAG TGAGGATGCT 
GTCAAGGCCC GCCTGGCTGC CTTAGCTGAC CAGTGGCAGT TCTTGGTGCA AAAGTCAGCG 
GAAAAGAGCC AGAAACTGAA AGAAGCCAAC AAGCAGCAGA ACTTCAACAC AGGGATCAAG 
GACTTTGACT TCTGGCTGTC TGAGGTGGAG GCCCTGCTGG CATCCGAAGA TTATGGCAAA 
GACCTAGCTT CTGTGAACAA CCTGCTGAAA AAGCATCAAC TGCTGGAAGC AGATATATCT 
GCCCATGAGG ATCGCCTGAA GGACCTGAAC AGCCAGGCAG ACAGCCTGAT GACCAGCAGT 
GCCTTCGACA CCTCCCAAGT AAAGGACAAG AGGGACACCA TCAACGGGCG CTTCCAGAAG 
ATCAAGAGCA TGGCGGCCTC CCGGCGAGCC AAGCTGAATG AATCCCATCG CCTGCACCAG 
TTCTTCCGGG ACATGGATGA CGAGGAGTCC TGGATCAAGG AGAAGAAGCT GCTGGTGGGC 
TCAGAGGACT ACGGCCGGGA CCTAACCGGC GTGCAGAACC TGAGGAAGAA GCACAAGCGG 
CTGGAAGCAG AACTGGCTGC GCATGAGCCG GCTATTCAGG GTGTCCTGGA CACTGGCAAG 
AAGCTGTCCG ATGACAACAC CATCGGGAAA GAGGAGATCC AGCAGCGGCT GGCGCAGTTT 
GTGGAGCACT GGAAAGAGCT GAAGCAGCTG GCAGCTGCCC GGGGTCAGCG GCTGGAAGAG 
TCCTTGGAAT ATCAGCAGTT TGTAGCCAAT GTGGAAGAGG AAGAAGCCTG GATCAATGAG 
AAAATGACCC TGGTGGCCAG CGAAGATTAT GGCGACACTC TTGCCGCCAT CCAGGGCTTA 
CTGAAGAAAC ATGAAGCTTT TGAGACAGAC TTCACCGTCC ACAAGGATCG CGTGAATGAT 
GTCTGCACCA ATGGACAAGA CCTCATTAAG AAGAACAATC ACCATGAGGA GAACATCTCT 
TCAAAGATGA AGGGCCTGAA CGGGAAAGTG TCAGACCTGG AGAAAGCTGC AGCCCAGAGA 
AAGGCGAAGC TGGATGAGAA CTCGGCCTTC CTTCAGTTCA ACTGGAAGGC GGACGTGGTG 
GAGTCCTGGA TCGGTGAAAA GGAGAACAGC TTGAAGACAG ATGATTATGG CCGAGACCTG 
TCTTCTGTGC AGACGCTCCT CACCAAACAG GAAACTTTTG ACGCTGGGCT GCAGGCCTTC 
CAGCAGGAAG GCATTGCCAA CATCACTGCC CTCAAAGATC AGCTTCTCGC CGCCAAACAC 
GTTCAGTCCA AGGCCATCGA GGCCCGGCAC GCCTCCCTCA TGAAGAGGTG GAGCCAGCTT 
CTGGCCAACT CAGCCGCCCG CAAGAAGAAG CTTCTGGAGG CTCAGAGTCA CTTCCGCAAG 
GTGGAGGACC TCTTCCTGAC CTTCGCCAAA AAGGCTTCTG CCTTCAACAG CTGGTTTGAA 
AATGCAGAGG AGGACTTAAC AGACCCCGTG CGCTGCAACT CCTTGGAAGA AATCAAAGCT 
TTGCGCGAGG CCCACGACGC CTTCCGCTCC TCCCTCAGCT CTGCCCAGGC TGACTTCAAC 
CAGCTGGCCG AGCTGGACCG CCAGATCAAG AGCTTCCGCG TAGCCTCCAA CCCCTACACC 
TGGTTTACCA TGGAGGCCCT GGAGGAGACC TGGAGGAACC TACAGAAAAT CATCAAGGAG 
AGGGAGCTGG AGCTGCAGAA GGAACAGCGG CGGCAGGAGG AGAACGACAA GCTGCGCCAG 
GAGTTTGCCC AGCACGCCAA CGCCTTCCAC CAGTGGATCC AAGAGACCAG GACATACCTC 
CTCGATGGCA TAGCATATCG TCGGGTCATT CGTGTCTATC AGTATGAAGT TGGGGATGAT 
CTGTCTGGAA GGTCCTGTAT GGTGGAAGAG TCGGGGACCC TCGAATCCCA GCTTGAAGCT 
ACCAAACGCA AGCACCAGGA AATCCGAGCC ATGAGAAGTC AGCTCAAAAA GATCGAGGAC 
CTGGGGGCCG CCATGGAGGA GGCCCTCATC CTGGACAACA AGTACACGGA GCACAGCACC 
GTGGGCCTCG CCCAGCAGTG GGACCAGCTG GACCAGCTGG GCATGCGCAT GCAGCACAAC 
CTGGAGCAGC AGATCCAGGC CAGGAACACA ACAGGTGTGA CTGAGGAGGC CCTCAAAGAA 
TTCAGCATGA TGTTTAAACA CTTTGACAAG GACAAGTCTG GCAGGCTGAA CCATCAGGAG 
TTCAAATCTT GCCTGCGCTC CCTGGGCTAT GACCTGCCCA TGGTGGAGGA AGGGGAACCT 
GACCCTGAGT TCGAGGCAAT CCTGGACACG GTGGATCCGA ACAGAGATGG CCATGTCTCC 
TTGCAAGAAT ACATGGCTTT CATGATCAGC CGCGAAACTG AGAACGTCAA GTCCAGCGAG 
GAGATTGAGA GCGCCTTCCG GGCCCTCAGC TCAGAGGGAA AGCCTTACGT GACCAAGGAG 
GAGCTCTACC AGAACCTGAC CCGGGAACAA GCCGACTACT GCGTCTCCCA CATGAAGCCC 
TACGTGGACG GCAAGGGCCG CGAGCTCCCC ACCGCGTTCG ACTACGTGGA GTTCACCCGC 
TCGCTTTTCG TGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_006717247.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006717310.1 Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X4, mRNA.

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CloneID	OHu43455
Clone ID Related Accession (Same CDS sequence)	XM_006717247.2
Accession Version	XM_006717247.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7473bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006717247.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACCG ATACCACCGC 
TTCAAGGAAC TCTCAACCCT TAGGCGTCAG AAGCTGGAAG ATTCCTATCG ATTCCAGTTC 
TTTCAAAGAG ATGCTGAAGA GCTGGAGAAA TGGATACAGG AAAAACTTCA GATTGCATCT 
GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGAAAGC TTCAGAAGCA TCAAGCATTT 
GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAGC TGGATGAAAC TGGAAACCTG 
ATGATCTCAG AAGGGCATTT TGCATCTGAA ACCATACGGA CCCGTTTGAT GGAGCTGCAC 
CGCCAGTGGG AATTACTTTT GGAGAAGATG CGAGAAAAAG GAATCAAACT GCTGCAGGCC 
CAGAAGTTGG TGCAGTACTT ACGAGAATGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCAATTG TTACTTCTGA AGAGCTGGGC CAGGATCTGG AGCATGTAGA GGTTTTACAG 
AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCTCATG AAGAAAGAGT TAATGAAGTG 
AACCAGTTTG CTGCCAAACT CATACAGGAG CAGCACCCTG AGGAGGAACT GATCAAGACT 
AAGCAGGATG AAGTCAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCTCT GCAGAGGCAG 
GGGAAGCTCT TTGGGGCAGC AGAAGTTCAG CGCTTTAACA GGGATGTGGA TGAGACTATC 
AGTTGGATTA AGGAAAAGGA GCAGTTAATG GCCTCTGATG ATTTTGGCCG AGACCTGGCA 
AGTGTTCAGG CTCTGCTTCG GAAGCACGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAA 
GACAAGGTCA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCCCA CCCTCTGAGT 
GCAACACAGA TTCAAGTGAA GCGAGAGGAA CTGATTACAA ACTGGGAGCA GATCCGCACC 
TTGGCGGCAG AGAGACATGC ACGGCTCAAT GATTCATACA GGCTTCAACG CTTCCTTGCT 
GACTTCCGTG ACCTCACCAG CTGGGTGACT GAGATGAAAG CCCTCATCAA TGCAGATGAG 
CTTGCCAGTG ATGTGGCTGG GGCTGAAGCC CTGCTAGATA GACACCAAGA GCACAAGGGT 
GAAATTGATG CCCATGAAGA CAGCTTCAAA TCTGCAGATG AATCTGGACA GGCACTGCTT 
GCTGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCGTCCT TTCCGAGGAG 
AGAGCGGCGC TGCTGGAGCT GTGGGAGCTG CGCAGGCAGC AGTACGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGGGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ATGAAGACTT GGGAGATTCC TTGGATAGTG TGGAAGCGCT TCTTAAGAAG 
CACGAAGACT TTGAGAAATC CCTTAGTGCC CAGGAGGAAA AGATTACAGC ATTAGATGAA 
TTTGCAACCA AGCTAATTCA GAACAACCAC TATGCAATGG AAGATGTGGC CACTCGCCGA 
GATGCTCTGT TGAGCCGCCG CAATGCCCTT CACGAGAGAG CCATGCGTCG CCGGGCCCAG 
CTAGCCGATT CTTTCCATCT GCAGCAGTTT TTCCGTGATT CTGATGAGCT CAAGAGTTGG 
GTCAATGAGA AGATGAAAAC TGCCACAGAT GAAGCTTATA AAGATCCATC CAACCTACAA 
GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CAGCAAACCA GAGCCGAATT 
GATGCCTTGG AGAAAGCTGG CCAAAAGCTG ATTGATGTCA ACCACTATGC CAAGGATGAA 
GTGGCAGCTC GTATGAATGA GGTGATCAGT TTGTGGAAGA AACTGCTAGA GGCCACTGAA 
CTGAAAGGAA TAAAGCTTCG TGAAGCCAAC CAGCAACAGC AATTTAATCG CAATGTTGAG 
GATATTGAAT TGTGGCTATA TGAAGTAGAA GGTCACTTGG CTTCGGATGA TTACGGCAAA 
GATCTTACCA ATGTGCAGAA CCTCCAGAAG AAACATGCAC TGCTAGAGGC AGATGTGGCT 
GCTCACCAGG ACCGAATTGA TGGCATCACC ATTCAGGCCC GCCAGTTCCA AGATGCTGGC 
CATTTTGATG CAGAAAACAT CAAGAAGAAA CAGGAAGCCC TCGTGGCTCG CTATGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCTCTGCG GTTGCAGCAG 
CTCTTCCGGG ATGTTGAGGA TGAGGAGACG TGGATTCGAG AGAAAGAGCC CATTGCCGCA 
TCTACCAACA GAGGTAAGGA TTTAATTGGG GTCCAGAATC TGCTAAAGAA ACATCAAGCC 
TTACAAGCAG AAATTGCTGG ACATGAACCA CGCATCAAAG CAGTTACACA GAAGGGGAAT 
GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAGCT TCACGAGCTG 
AACCAAAAGT GGGAGGCACT GAAAGCCAAA GCTTCCCAGC GTCGGCAGGA CCTGGAGGAC 
TCTCTGCAGG CCCAGCAGTA CTTTGCTGAT GCTAACGAGG CTGAATCCTG GATGCGGGAG 
AAGGAACCCA TTGTGGGCAG CACTGACTAT GGCAAGGACG AAGACTCTGC TGAGGCTCTA 
CTGAAGAAAC ACGAAGCTTT GATGTCAGAT CTCAGTGCCT ACGGCAGCAG CATCCAGGCT 
TTGCGAGAAC AAGCACAGTC CTGCCGGCAA CAAGTGGCCC CCACGGATGA TGAGACTGGG 
AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAGGGAG ATATCCTTAC CTTACTCAAC AGCACCAACA AGGATTGGTG GAAAGTGGAA 
GTGAACGATC GTCAGGGTTT TGTGCCGGCT GCGTACGTGA AGAAATTGGA CCCCGCCCAG 
TCAGCCTCCC GGGAGAATCT CCTGGAGGAG CAAGGCAGCA TAGCACTGCG GCAGGAGCAG 
ATTGACAATC AATATCATTC TCTGCTGGAA CTGGGTGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGTTGCA AGAAGTTTAT GTTGTTCCGT GAAGCGAATG AACTACAGCA ATGGATCAAT 
GAGAAGGAAG CCGCTCTGAC AAGTGAGGAG GTCGGAGCAG ACTTGGAGCA GGTTGAGGTG 
CTCCAGAAGA AGTTTGATGA CTTCCAGAAG GACCTGAAGG CCAATGAGTC ACGGTTGAAG 
GACATTAACA AGGTAGCTGA AGACCTGGAG TCTGAAGGTC TCATGGCAGA GGAGGTGCAG 
GCTGTGCAAC AACAGGAAGT GTATGGCATG ATGCCCAGGG ATGAAACTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGTCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTGA ATGAGCGCTG GCGGTCCCTA CAGCAGCTGG CCGAGGAACG GAGCCAGCTC 
TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CTGATGAAAC CAAAGAATGG 
ATTGAAGAGA AGAATCAAGC TCTAAACACA GACAATTATG GACATGATCT CGCCAGTGTC 
CAGGCCCTGC AACGCAAGCA TGAGGGCTTC GAGAGGGACC TTGCGGCTCT CGGTGACAAG 
GTAAACTCCC TTGGTGAAAC AGCAGAGCGC CTGATCCAGT CCCATCCCGA GTCAGCAGAA 
GACCTGCAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA GCAGCCTGGG GAAACGTGCA 
GATCAGCGCA AGGCAAAGTT GGGTGACTCC CACGACCTGC AGCGCTTCCT TAGCGATTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATA CGGGGGTTGG TGTCCTCAGA TGAGCTAGCC 
AAGGATGTCA CCGGAGCTGA GGCATTGCTG GAGCGACACC AGGAACACCG GACAGAAATC 
GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GACAGCAGCT GTTGGCTCAC 
GGACACTATG CCAGCCCTGA GATCAAGCAG AAACTTGATA TTCTTGACCA GGAGCGTGCA 
GACCTGGAGA AGGCCTGGGT TCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAACTGCAG 
CTGTTCCATC GGGACTGTGA GCAAGCTGAG AACTGGATGG CTGCCCGGGA GGCCTTCTTG 
AATACCGAAG ACAAAGGAGA CTCACTGGAC AGCGTAGAGG CTCTGATCAA AAAACATGAA 
GACTTTGACA AAGCGATTAA CGTCCAGGAA GAGAAGATTG CTGCTCTGCA GGCCTTTGCC 
GACCAGCTCA TCGCTGCCGG CCATTATGCC AAGGGAGACA TTTCTAGCCG GCGCAATGAG 
GTCTTGGACA GGTGGCGACG TCTGAAAGCC CAGATGATTG AGAAAAGGTC AAAGCTAGGA 
GAATCTCAAA CCCTCCAACA GTTCAGCCGG GATGTGGATG AGATTGAGGC TTGGATCAGT 
GAAAAATTGC AAACAGCGAG TGATGAGTCG TACAAGGATC CCACCAACAT CCAGCTTTCC 
AAGCTGCTGA GCAAGCACCA GAAGCACCAG GCTTTTGAAG CAGAGCTGCA TGCCAACGCT 
GACCGGATCC GTGGGGTTAT CGACATGGGC AACTCCCTCA TTGAACGTGG AGCCTGTGCC 
GGCAGTGAGG ATGCTGTCAA GGCCCGCCTG GCTGCCTTAG CTGACCAGTG GCAGTTCTTG 
GTGCAAAAGT CAGCGGAAAA GAGCCAGAAA CTGAAAGAAG CCAACAAGCA GCAGAACTTC 
AACACAGGGA TCAAGGACTT TGACTTCTGG CTGTCTGAGG TGGAGGCCCT GCTGGCATCC 
GAAGATTATG GCAAAGACCT AGCTTCTGTG AACAACCTGC TGAAAAAGCA TCAACTGCTG 
GAAGCAGATA TATCTGCCCA TGAGGATCGC CTGAAGGACC TGAACAGCCA GGCAGACAGC 
CTGATGACCA GCAGTGCCTT CGACACCTCC CAAGTAAAGG ACAAGAGGGA CACCATCAAC 
GGGCGCTTCC AGAAGATCAA GAGCATGGCG GCCTCCCGGC GAGCCAAGCT GAATGAATCC 
CATCGCCTGC ACCAGTTCTT CCGGGACATG GATGACGAGG AGTCCTGGAT CAAGGAGAAG 
AAGCTGCTGG TGGGCTCAGA GGACTACGGC CGGGACCTAA CCGGCGTGCA GAACCTGAGG 
AAGAAGCACA AGCGGCTGGA AGCAGAACTG GCTGCGCATG AGCCGGCTAT TCAGGGTGTC 
CTGGACACTG GCAAGAAGCT GTCCGATGAC AACACCATCG GGAAAGAGGA GATCCAGCAG 
CGGCTGGCGC AGTTTGTGGA GCACTGGAAA GAGCTGAAGC AGCTGGCAGC TGCCCGGGGT 
CAGCGGCTGG AAGAGTCCTT GGAATATCAG CAGTTTGTAG CCAATGTGGA AGAGGAAGAA 
GCCTGGATCA ATGAGAAAAT GACCCTGGTG GCCAGCGAAG ATTATGGCGA CACTCTTGCC 
GCCATCCAGG GCTTACTGAA GAAACATGAA GCTTTTGAGA CAGACTTCAC CGTCCACAAG 
GATCGCGTGA ATGATGTCTG CACCAATGGA CAAGACCTCA TTAAGAAGAA CAATCACCAT 
GAGGAGAACA TCTCTTCAAA GATGAAGGGC CTGAACGGGA AAGTGTCAGA CCTGGAGAAA 
GCTGCAGCCC AGAGAAAGGC GAAGCTGGAT GAGAACTCGG CCTTCCTTCA GTTCAACTGG 
AAGGCGGACG TGGTGGAGTC CTGGATCGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT 
TATGGCCGAG ACCTGTCTTC TGTGCAGACG CTCCTCACCA AACAGGAAAC TTTTGACGCT 
GGGCTGCAGG CCTTCCAGCA GGAAGGCATT GCCAACATCA CTGCCCTCAA AGATCAGCTT 
CTCGCCGCCA AACACGTTCA GTCCAAGGCC ATCGAGGCCC GGCACGCCTC CCTCATGAAG 
AGGTGGAGCC AGCTTCTGGC CAACTCAGCC GCCCGCAAGA AGAAGCTTCT GGAGGCTCAG 
AGTCACTTCC GCAAGGTGGA GGACCTCTTC CTGACCTTCG CCAAAAAGGC TTCTGCCTTC 
AACAGCTGGT TTGAAAATGC AGAGGAGGAC TTAACAGACC CCGTGCGCTG CAACTCCTTG 
GAAGAAATCA AAGCTTTGCG CGAGGCCCAC GACGCCTTCC GCTCCTCCCT CAGCTCTGCC 
CAGGCTGACT TCAACCAGCT GGCCGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTAGCC 
TCCAACCCCT ACACCTGGTT TACCATGGAG GCCCTGGAGG AGACCTGGAG GAACCTACAG 
AAAATCATCA AGGAGAGGGA GCTGGAGCTG CAGAAGGAAC AGCGGCGGCA GGAGGAGAAC 
GACAAGCTGC GCCAGGAGTT TGCCCAGCAC GCCAACGCCT TCCACCAGTG GATCCAAGAG 
ACCAGGACAT ACCTCCTCGA TGGCATAGCA TATCGTCGGG TCATTCGTGT CTATCAGTAT 
GAAGTTGGGG ATGATCTGTC TGGAAGGTCC TGTATGGTGG AAGAGTCGGG GACCCTCGAA 
TCCCAGCTTG AAGCTACCAA ACGCAAGCAC CAGGAAATCC GAGCCATGAG AAGTCAGCTC 
AAAAAGATCG AGGACCTGGG GGCCGCCATG GAGGAGGCCC TCATCCTGGA CAACAAGTAC 
ACGGAGCACA GCACCGTGGG CCTCGCCCAG CAGTGGGACC AGCTGGACCA GCTGGGCATG 
CGCATGCAGC ACAACCTGGA GCAGCAGATC CAGGCCAGGA ACACAACAGG TGTGACTGAG 
GAGGCCCTCA AAGAATTCAG CATGATGTTT AAACACTTTG ACAAGGACAA GTCTGGCAGG 
CTGAACCATC AGGAGTTCAA ATCTTGCCTG CGCTCCCTGG GCTATGACCT GCCCATGGTG 
GAGGAAGGGG AACCTGACCC TGAGTTCGAG GCAATCCTGG ACACGGTGGA TCCGAACAGA 
GATGGCCATG TCTCCTTGCA AGAATACATG GCTTTCATGA TCAGCCGCGA AACTGAGAAC 
GTCAAGTCCA GCGAGGAGAT TGAGAGCGCC TTCCGGGCCC TCAGCTCAGA GGGAAAGCCT 
TACGTGACCA AGGAGGAGCT CTACCAGAAC CTGACCCGGG AACAAGCCGA CTACTGCGTC 
TCCCACATGA AGCCCTACGT GGACGGCAAG GGCCGCGAGC TCCCCACCGC GTTCGACTAC 
GTGGAGTTCA CCCGCTCGCT TTTCGTGAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006717310.1
CDS	14..7486
Translation	MLSSFRKRRVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELASDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMRRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPTDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGMMPRDETDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKQKLDILDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAAGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINGRFQKIKSMAASRRAKLNESHRLHQFFRDMDDEESWIKEKKLLVGSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHASLMKRWSQLLANSAARKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGIAYRRVIRVYQYEVGDDLSGRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_006717247.2
Organism	Homo sapiens(human)
Definition	Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006717247.2

ORF Insert Sequence:

ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACCG ATACCACCGC 
TTCAAGGAAC TCTCAACCCT TAGGCGTCAG AAGCTGGAAG ATTCCTATCG ATTCCAGTTC 
TTTCAAAGAG ATGCTGAAGA GCTGGAGAAA TGGATACAGG AAAAACTTCA GATTGCATCT 
GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGAAAGC TTCAGAAGCA TCAAGCATTT 
GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAGC TGGATGAAAC TGGAAACCTG 
ATGATCTCAG AAGGGCATTT TGCATCTGAA ACCATACGGA CCCGTTTGAT GGAGCTGCAC 
CGCCAGTGGG AATTACTTTT GGAGAAGATG CGAGAAAAAG GAATCAAACT GCTGCAGGCC 
CAGAAGTTGG TGCAGTACTT ACGAGAATGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCAATTG TTACTTCTGA AGAGCTGGGC CAGGATCTGG AGCATGTAGA GGTTTTACAG 
AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCTCATG AAGAAAGAGT TAATGAAGTG 
AACCAGTTTG CTGCCAAACT CATACAGGAG CAGCACCCTG AGGAGGAACT GATCAAGACT 
AAGCAGGATG AAGTCAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCTCT GCAGAGGCAG 
GGGAAGCTCT TTGGGGCAGC AGAAGTTCAG CGCTTTAACA GGGATGTGGA TGAGACTATC 
AGTTGGATTA AGGAAAAGGA GCAGTTAATG GCCTCTGATG ATTTTGGCCG AGACCTGGCA 
AGTGTTCAGG CTCTGCTTCG GAAGCACGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAA 
GACAAGGTCA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCCCA CCCTCTGAGT 
GCAACACAGA TTCAAGTGAA GCGAGAGGAA CTGATTACAA ACTGGGAGCA GATCCGCACC 
TTGGCGGCAG AGAGACATGC ACGGCTCAAT GATTCATACA GGCTTCAACG CTTCCTTGCT 
GACTTCCGTG ACCTCACCAG CTGGGTGACT GAGATGAAAG CCCTCATCAA TGCAGATGAG 
CTTGCCAGTG ATGTGGCTGG GGCTGAAGCC CTGCTAGATA GACACCAAGA GCACAAGGGT 
GAAATTGATG CCCATGAAGA CAGCTTCAAA TCTGCAGATG AATCTGGACA GGCACTGCTT 
GCTGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCGTCCT TTCCGAGGAG 
AGAGCGGCGC TGCTGGAGCT GTGGGAGCTG CGCAGGCAGC AGTACGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGGGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ATGAAGACTT GGGAGATTCC TTGGATAGTG TGGAAGCGCT TCTTAAGAAG 
CACGAAGACT TTGAGAAATC CCTTAGTGCC CAGGAGGAAA AGATTACAGC ATTAGATGAA 
TTTGCAACCA AGCTAATTCA GAACAACCAC TATGCAATGG AAGATGTGGC CACTCGCCGA 
GATGCTCTGT TGAGCCGCCG CAATGCCCTT CACGAGAGAG CCATGCGTCG CCGGGCCCAG 
CTAGCCGATT CTTTCCATCT GCAGCAGTTT TTCCGTGATT CTGATGAGCT CAAGAGTTGG 
GTCAATGAGA AGATGAAAAC TGCCACAGAT GAAGCTTATA AAGATCCATC CAACCTACAA 
GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CAGCAAACCA GAGCCGAATT 
GATGCCTTGG AGAAAGCTGG CCAAAAGCTG ATTGATGTCA ACCACTATGC CAAGGATGAA 
GTGGCAGCTC GTATGAATGA GGTGATCAGT TTGTGGAAGA AACTGCTAGA GGCCACTGAA 
CTGAAAGGAA TAAAGCTTCG TGAAGCCAAC CAGCAACAGC AATTTAATCG CAATGTTGAG 
GATATTGAAT TGTGGCTATA TGAAGTAGAA GGTCACTTGG CTTCGGATGA TTACGGCAAA 
GATCTTACCA ATGTGCAGAA CCTCCAGAAG AAACATGCAC TGCTAGAGGC AGATGTGGCT 
GCTCACCAGG ACCGAATTGA TGGCATCACC ATTCAGGCCC GCCAGTTCCA AGATGCTGGC 
CATTTTGATG CAGAAAACAT CAAGAAGAAA CAGGAAGCCC TCGTGGCTCG CTATGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCTCTGCG GTTGCAGCAG 
CTCTTCCGGG ATGTTGAGGA TGAGGAGACG TGGATTCGAG AGAAAGAGCC CATTGCCGCA 
TCTACCAACA GAGGTAAGGA TTTAATTGGG GTCCAGAATC TGCTAAAGAA ACATCAAGCC 
TTACAAGCAG AAATTGCTGG ACATGAACCA CGCATCAAAG CAGTTACACA GAAGGGGAAT 
GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAGCT TCACGAGCTG 
AACCAAAAGT GGGAGGCACT GAAAGCCAAA GCTTCCCAGC GTCGGCAGGA CCTGGAGGAC 
TCTCTGCAGG CCCAGCAGTA CTTTGCTGAT GCTAACGAGG CTGAATCCTG GATGCGGGAG 
AAGGAACCCA TTGTGGGCAG CACTGACTAT GGCAAGGACG AAGACTCTGC TGAGGCTCTA 
CTGAAGAAAC ACGAAGCTTT GATGTCAGAT CTCAGTGCCT ACGGCAGCAG CATCCAGGCT 
TTGCGAGAAC AAGCACAGTC CTGCCGGCAA CAAGTGGCCC CCACGGATGA TGAGACTGGG 
AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAGGGAG ATATCCTTAC CTTACTCAAC AGCACCAACA AGGATTGGTG GAAAGTGGAA 
GTGAACGATC GTCAGGGTTT TGTGCCGGCT GCGTACGTGA AGAAATTGGA CCCCGCCCAG 
TCAGCCTCCC GGGAGAATCT CCTGGAGGAG CAAGGCAGCA TAGCACTGCG GCAGGAGCAG 
ATTGACAATC AATATCATTC TCTGCTGGAA CTGGGTGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGTTGCA AGAAGTTTAT GTTGTTCCGT GAAGCGAATG AACTACAGCA ATGGATCAAT 
GAGAAGGAAG CCGCTCTGAC AAGTGAGGAG GTCGGAGCAG ACTTGGAGCA GGTTGAGGTG 
CTCCAGAAGA AGTTTGATGA CTTCCAGAAG GACCTGAAGG CCAATGAGTC ACGGTTGAAG 
GACATTAACA AGGTAGCTGA AGACCTGGAG TCTGAAGGTC TCATGGCAGA GGAGGTGCAG 
GCTGTGCAAC AACAGGAAGT GTATGGCATG ATGCCCAGGG ATGAAACTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGTCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTGA ATGAGCGCTG GCGGTCCCTA CAGCAGCTGG CCGAGGAACG GAGCCAGCTC 
TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CTGATGAAAC CAAAGAATGG 
ATTGAAGAGA AGAATCAAGC TCTAAACACA GACAATTATG GACATGATCT CGCCAGTGTC 
CAGGCCCTGC AACGCAAGCA TGAGGGCTTC GAGAGGGACC TTGCGGCTCT CGGTGACAAG 
GTAAACTCCC TTGGTGAAAC AGCAGAGCGC CTGATCCAGT CCCATCCCGA GTCAGCAGAA 
GACCTGCAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA GCAGCCTGGG GAAACGTGCA 
GATCAGCGCA AGGCAAAGTT GGGTGACTCC CACGACCTGC AGCGCTTCCT TAGCGATTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATA CGGGGGTTGG TGTCCTCAGA TGAGCTAGCC 
AAGGATGTCA CCGGAGCTGA GGCATTGCTG GAGCGACACC AGGAACACCG GACAGAAATC 
GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GACAGCAGCT GTTGGCTCAC 
GGACACTATG CCAGCCCTGA GATCAAGCAG AAACTTGATA TTCTTGACCA GGAGCGTGCA 
GACCTGGAGA AGGCCTGGGT TCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAACTGCAG 
CTGTTCCATC GGGACTGTGA GCAAGCTGAG AACTGGATGG CTGCCCGGGA GGCCTTCTTG 
AATACCGAAG ACAAAGGAGA CTCACTGGAC AGCGTAGAGG CTCTGATCAA AAAACATGAA 
GACTTTGACA AAGCGATTAA CGTCCAGGAA GAGAAGATTG CTGCTCTGCA GGCCTTTGCC 
GACCAGCTCA TCGCTGCCGG CCATTATGCC AAGGGAGACA TTTCTAGCCG GCGCAATGAG 
GTCTTGGACA GGTGGCGACG TCTGAAAGCC CAGATGATTG AGAAAAGGTC AAAGCTAGGA 
GAATCTCAAA CCCTCCAACA GTTCAGCCGG GATGTGGATG AGATTGAGGC TTGGATCAGT 
GAAAAATTGC AAACAGCGAG TGATGAGTCG TACAAGGATC CCACCAACAT CCAGCTTTCC 
AAGCTGCTGA GCAAGCACCA GAAGCACCAG GCTTTTGAAG CAGAGCTGCA TGCCAACGCT 
GACCGGATCC GTGGGGTTAT CGACATGGGC AACTCCCTCA TTGAACGTGG AGCCTGTGCC 
GGCAGTGAGG ATGCTGTCAA GGCCCGCCTG GCTGCCTTAG CTGACCAGTG GCAGTTCTTG 
GTGCAAAAGT CAGCGGAAAA GAGCCAGAAA CTGAAAGAAG CCAACAAGCA GCAGAACTTC 
AACACAGGGA TCAAGGACTT TGACTTCTGG CTGTCTGAGG TGGAGGCCCT GCTGGCATCC 
GAAGATTATG GCAAAGACCT AGCTTCTGTG AACAACCTGC TGAAAAAGCA TCAACTGCTG 
GAAGCAGATA TATCTGCCCA TGAGGATCGC CTGAAGGACC TGAACAGCCA GGCAGACAGC 
CTGATGACCA GCAGTGCCTT CGACACCTCC CAAGTAAAGG ACAAGAGGGA CACCATCAAC 
GGGCGCTTCC AGAAGATCAA GAGCATGGCG GCCTCCCGGC GAGCCAAGCT GAATGAATCC 
CATCGCCTGC ACCAGTTCTT CCGGGACATG GATGACGAGG AGTCCTGGAT CAAGGAGAAG 
AAGCTGCTGG TGGGCTCAGA GGACTACGGC CGGGACCTAA CCGGCGTGCA GAACCTGAGG 
AAGAAGCACA AGCGGCTGGA AGCAGAACTG GCTGCGCATG AGCCGGCTAT TCAGGGTGTC 
CTGGACACTG GCAAGAAGCT GTCCGATGAC AACACCATCG GGAAAGAGGA GATCCAGCAG 
CGGCTGGCGC AGTTTGTGGA GCACTGGAAA GAGCTGAAGC AGCTGGCAGC TGCCCGGGGT 
CAGCGGCTGG AAGAGTCCTT GGAATATCAG CAGTTTGTAG CCAATGTGGA AGAGGAAGAA 
GCCTGGATCA ATGAGAAAAT GACCCTGGTG GCCAGCGAAG ATTATGGCGA CACTCTTGCC 
GCCATCCAGG GCTTACTGAA GAAACATGAA GCTTTTGAGA CAGACTTCAC CGTCCACAAG 
GATCGCGTGA ATGATGTCTG CACCAATGGA CAAGACCTCA TTAAGAAGAA CAATCACCAT 
GAGGAGAACA TCTCTTCAAA GATGAAGGGC CTGAACGGGA AAGTGTCAGA CCTGGAGAAA 
GCTGCAGCCC AGAGAAAGGC GAAGCTGGAT GAGAACTCGG CCTTCCTTCA GTTCAACTGG 
AAGGCGGACG TGGTGGAGTC CTGGATCGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT 
TATGGCCGAG ACCTGTCTTC TGTGCAGACG CTCCTCACCA AACAGGAAAC TTTTGACGCT 
GGGCTGCAGG CCTTCCAGCA GGAAGGCATT GCCAACATCA CTGCCCTCAA AGATCAGCTT 
CTCGCCGCCA AACACGTTCA GTCCAAGGCC ATCGAGGCCC GGCACGCCTC CCTCATGAAG 
AGGTGGAGCC AGCTTCTGGC CAACTCAGCC GCCCGCAAGA AGAAGCTTCT GGAGGCTCAG 
AGTCACTTCC GCAAGGTGGA GGACCTCTTC CTGACCTTCG CCAAAAAGGC TTCTGCCTTC 
AACAGCTGGT TTGAAAATGC AGAGGAGGAC TTAACAGACC CCGTGCGCTG CAACTCCTTG 
GAAGAAATCA AAGCTTTGCG CGAGGCCCAC GACGCCTTCC GCTCCTCCCT CAGCTCTGCC 
CAGGCTGACT TCAACCAGCT GGCCGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTAGCC 
TCCAACCCCT ACACCTGGTT TACCATGGAG GCCCTGGAGG AGACCTGGAG GAACCTACAG 
AAAATCATCA AGGAGAGGGA GCTGGAGCTG CAGAAGGAAC AGCGGCGGCA GGAGGAGAAC 
GACAAGCTGC GCCAGGAGTT TGCCCAGCAC GCCAACGCCT TCCACCAGTG GATCCAAGAG 
ACCAGGACAT ACCTCCTCGA TGGCATAGCA TATCGTCGGG TCATTCGTGT CTATCAGTAT 
GAAGTTGGGG ATGATCTGTC TGGAAGGTCC TGTATGGTGG AAGAGTCGGG GACCCTCGAA 
TCCCAGCTTG AAGCTACCAA ACGCAAGCAC CAGGAAATCC GAGCCATGAG AAGTCAGCTC 
AAAAAGATCG AGGACCTGGG GGCCGCCATG GAGGAGGCCC TCATCCTGGA CAACAAGTAC 
ACGGAGCACA GCACCGTGGG CCTCGCCCAG CAGTGGGACC AGCTGGACCA GCTGGGCATG 
CGCATGCAGC ACAACCTGGA GCAGCAGATC CAGGCCAGGA ACACAACAGG TGTGACTGAG 
GAGGCCCTCA AAGAATTCAG CATGATGTTT AAACACTTTG ACAAGGACAA GTCTGGCAGG 
CTGAACCATC AGGAGTTCAA ATCTTGCCTG CGCTCCCTGG GCTATGACCT GCCCATGGTG 
GAGGAAGGGG AACCTGACCC TGAGTTCGAG GCAATCCTGG ACACGGTGGA TCCGAACAGA 
GATGGCCATG TCTCCTTGCA AGAATACATG GCTTTCATGA TCAGCCGCGA AACTGAGAAC 
GTCAAGTCCA GCGAGGAGAT TGAGAGCGCC TTCCGGGCCC TCAGCTCAGA GGGAAAGCCT 
TACGTGACCA AGGAGGAGCT CTACCAGAAC CTGACCCGGG AACAAGCCGA CTACTGCGTC 
TCCCACATGA AGCCCTACGT GGACGGCAAG GGCCGCGAGC TCCCCACCGC GTTCGACTAC 
GTGGAGTTCA CCCGCTCGCT TTTCGTGAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_006717248.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006717311.1 Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X5, mRNA.

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CloneID	OHu43456
Clone ID Related Accession (Same CDS sequence)	XM_006717248.2 , NM_001375312.2
Accession Version	XM_006717248.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7470bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-12
Organism	Homo sapiens(human)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006717248.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END##

ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACCG ATACCACCGC 
TTCAAGGAAC TCTCAACCCT TAGGCGTCAG AAGCTGGAAG ATTCCTATCG ATTCCAGTTC 
TTTCAAAGAG ATGCTGAAGA GCTGGAGAAA TGGATACAGG AAAAACTTCA GATTGCATCT 
GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGAAAGC TTCAGAAGCA TCAAGCATTT 
GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAGC TGGATGAAAC TGGAAACCTG 
ATGATCTCAG AAGGGCATTT TGCATCTGAA ACCATACGGA CCCGTTTGAT GGAGCTGCAC 
CGCCAGTGGG AATTACTTTT GGAGAAGATG CGAGAAAAAG GAATCAAACT GCTGCAGGCC 
CAGAAGTTGG TGCAGTACTT ACGAGAATGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCAATTG TTACTTCTGA AGAGCTGGGC CAGGATCTGG AGCATGTAGA GGTTTTACAG 
AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCTCATG AAGAAAGAGT TAATGAAGTG 
AACCAGTTTG CTGCCAAACT CATACAGGAG CAGCACCCTG AGGAGGAACT GATCAAGACT 
AAGCAGGATG AAGTCAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCTCT GCAGAGGCAG 
GGGAAGCTCT TTGGGGCAGC AGAAGTTCAG CGCTTTAACA GGGATGTGGA TGAGACTATC 
AGTTGGATTA AGGAAAAGGA GCAGTTAATG GCCTCTGATG ATTTTGGCCG AGACCTGGCA 
AGTGTTCAGG CTCTGCTTCG GAAGCACGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAA 
GACAAGGTCA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCCCA CCCTCTGAGT 
GCAACACAGA TTCAAGTGAA GCGAGAGGAA CTGATTACAA ACTGGGAGCA GATCCGCACC 
TTGGCGGCAG AGAGACATGC ACGGCTCAAT GATTCATACA GGCTTCAACG CTTCCTTGCT 
GACTTCCGTG ACCTCACCAG CTGGGTGACT GAGATGAAAG CCCTCATCAA TGCAGATGAG 
CTTGCCAGTG ATGTGGCTGG GGCTGAAGCC CTGCTAGATA GACACCAAGA GCACAAGGGT 
GAAATTGATG CCCATGAAGA CAGCTTCAAA TCTGCAGATG AATCTGGACA GGCACTGCTT 
GCTGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCGTCCT TTCCGAGGAG 
AGAGCGGCGC TGCTGGAGCT GTGGGAGCTG CGCAGGCAGC AGTACGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGGGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ATGAAGACTT GGGAGATTCC TTGGATAGTG TGGAAGCGCT TCTTAAGAAG 
CACGAAGACT TTGAGAAATC CCTTAGTGCC CAGGAGGAAA AGATTACAGC ATTAGATGAA 
TTTGCAACCA AGCTAATTCA GAACAACCAC TATGCAATGG AAGATGTGGC CACTCGCCGA 
GATGCTCTGT TGAGCCGCCG CAATGCCCTT CACGAGAGAG CCATGCGTCG CCGGGCCCAG 
CTAGCCGATT CTTTCCATCT GCAGCAGTTT TTCCGTGATT CTGATGAGCT CAAGAGTTGG 
GTCAATGAGA AGATGAAAAC TGCCACAGAT GAAGCTTATA AAGATCCATC CAACCTACAA 
GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CAGCAAACCA GAGCCGAATT 
GATGCCTTGG AGAAAGCTGG CCAAAAGCTG ATTGATGTCA ACCACTATGC CAAGGATGAA 
GTGGCAGCTC GTATGAATGA GGTGATCAGT TTGTGGAAGA AACTGCTAGA GGCCACTGAA 
CTGAAAGGAA TAAAGCTTCG TGAAGCCAAC CAGCAACAGC AATTTAATCG CAATGTTGAG 
GATATTGAAT TGTGGCTATA TGAAGTAGAA GGTCACTTGG CTTCGGATGA TTACGGCAAA 
GATCTTACCA ATGTGCAGAA CCTCCAGAAG AAACATGCAC TGCTAGAGGC AGATGTGGCT 
GCTCACCAGG ACCGAATTGA TGGCATCACC ATTCAGGCCC GCCAGTTCCA AGATGCTGGC 
CATTTTGATG CAGAAAACAT CAAGAAGAAA CAGGAAGCCC TCGTGGCTCG CTATGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCTCTGCG GTTGCAGCAG 
CTCTTCCGGG ATGTTGAGGA TGAGGAGACG TGGATTCGAG AGAAAGAGCC CATTGCCGCA 
TCTACCAACA GAGGTAAGGA TTTAATTGGG GTCCAGAATC TGCTAAAGAA ACATCAAGCC 
TTACAAGCAG AAATTGCTGG ACATGAACCA CGCATCAAAG CAGTTACACA GAAGGGGAAT 
GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAGCT TCACGAGCTG 
AACCAAAAGT GGGAGGCACT GAAAGCCAAA GCTTCCCAGC GTCGGCAGGA CCTGGAGGAC 
TCTCTGCAGG CCCAGCAGTA CTTTGCTGAT GCTAACGAGG CTGAATCCTG GATGCGGGAG 
AAGGAACCCA TTGTGGGCAG CACTGACTAT GGCAAGGACG AAGACTCTGC TGAGGCTCTA 
CTGAAGAAAC ACGAAGCTTT GATGTCAGAT CTCAGTGCCT ACGGCAGCAG CATCCAGGCT 
TTGCGAGAAC AAGCACAGTC CTGCCGGCAA CAAGTGGCCC CCACGGATGA TGAGACTGGG 
AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAGGGAG ATATCCTTAC CTTACTCAAC AGCACCAACA AGGATTGGTG GAAAGTGGAA 
GTGAACGATC GTCAGGGTTT TGTGCCGGCT GCGTACGTGA AGAAATTGGA CCCCGCCCAG 
TCAGCCTCCC GGGAGAATCT CCTGGAGGAG CAAGGCAGCA TAGCACTGCG GCAGGAGCAG 
ATTGACAATC AGACACGCAT AACTAAGGAG GCCGGCAGTG TATCTCTGCG TATGAAGCAG 
GTGGAAGAAC TATATCATTC TCTGCTGGAA CTGGGTGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGTTGCA AGAAGTTTAT GTTGTTCCGT GAAGCGAATG AACTACAGCA ATGGATCAAT 
GAGAAGGAAG CCGCTCTGAC AAGTGAGGAG GTCGGAGCAG ACTTGGAGCA GGTTGAGGTG 
CTCCAGAAGA AGTTTGATGA CTTCCAGAAG GACCTGAAGG CCAATGAGTC ACGGTTGAAG 
GACATTAACA AGGTAGCTGA AGACCTGGAG TCTGAAGGTC TCATGGCAGA GGAGGTGCAG 
GCTGTGCAAC AACAGGAAGT GTATGGCATG ATGCCCAGGG ATGAAACTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGTCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTGA ATGAGCGCTG GCGGTCCCTA CAGCAGCTGG CCGAGGAACG GAGCCAGCTC 
TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CTGATGAAAC CAAAGAATGG 
ATTGAAGAGA AGAATCAAGC TCTAAACACA GACAATTATG GACATGATCT CGCCAGTGTC 
CAGGCCCTGC AACGCAAGCA TGAGGGCTTC GAGAGGGACC TTGCGGCTCT CGGTGACAAG 
GTAAACTCCC TTGGTGAAAC AGCAGAGCGC CTGATCCAGT CCCATCCCGA GTCAGCAGAA 
GACCTGCAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA GCAGCCTGGG GAAACGTGCA 
GATCAGCGCA AGGCAAAGTT GGGTGACTCC CACGACCTGC AGCGCTTCCT TAGCGATTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATA CGGGGGTTGG TGTCCTCAGA TGAGCTAGCC 
AAGGATGTCA CCGGAGCTGA GGCATTGCTG GAGCGACACC AGGAACACCG GACAGAAATC 
GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GACAGCAGCT GTTGGCTCAC 
GGACACTATG CCAGCCCTGA GATCAAGCAG AAACTTGATA TTCTTGACCA GGAGCGTGCA 
GACCTGGAGA AGGCCTGGGT TCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAACTGCAG 
CTGTTCCATC GGGACTGTGA GCAAGCTGAG AACTGGATGG CTGCCCGGGA GGCCTTCTTG 
AATACCGAAG ACAAAGGAGA CTCACTGGAC AGCGTAGAGG CTCTGATCAA AAAACATGAA 
GACTTTGACA AAGCGATTAA CGTCCAGGAA GAGAAGATTG CTGCTCTGCA GGCCTTTGCC 
GACCAGCTCA TCGCTGCCGG CCATTATGCC AAGGGAGACA TTTCTAGCCG GCGCAATGAG 
GTCTTGGACA GGTGGCGACG TCTGAAAGCC CAGATGATTG AGAAAAGGTC AAAGCTAGGA 
GAATCTCAAA CCCTCCAACA GTTCAGCCGG GATGTGGATG AGATTGAGGC TTGGATCAGT 
GAAAAATTGC AAACAGCGAG TGATGAGTCG TACAAGGATC CCACCAACAT CCAGCTTTCC 
AAGCTGCTGA GCAAGCACCA GAAGCACCAG GCTTTTGAAG CAGAGCTGCA TGCCAACGCT 
GACCGGATCC GTGGGGTTAT CGACATGGGC AACTCCCTCA TTGAACGTGG AGCCTGTGCC 
GGCAGTGAGG ATGCTGTCAA GGCCCGCCTG GCTGCCTTAG CTGACCAGTG GCAGTTCTTG 
GTGCAAAAGT CAGCGGAAAA GAGCCAGAAA CTGAAAGAAG CCAACAAGCA GCAGAACTTC 
AACACAGGGA TCAAGGACTT TGACTTCTGG CTGTCTGAGG TGGAGGCCCT GCTGGCATCC 
GAAGATTATG GCAAAGACCT AGCTTCTGTG AACAACCTGC TGAAAAAGCA TCAACTGCTG 
GAAGCAGATA TATCTGCCCA TGAGGATCGC CTGAAGGACC TGAACAGCCA GGCAGACAGC 
CTGATGACCA GCAGTGCCTT CGACACCTCC CAAGTAAAGG ACAAGAGGGA CACCATCAAC 
GGGCGCTTCC AGAAGATCAA GAGCATGGCG GCCTCCCGGC GAGCCAAGCT GAATGAATCC 
CATCGCCTGC ACCAGTTCTT CCGGGACATG GATGACGAGG AGTCCTGGAT CAAGGAGAAG 
AAGCTGCTGG TGGGCTCAGA GGACTACGGC CGGGACCTAA CCGGCGTGCA GAACCTGAGG 
AAGAAGCACA AGCGGCTGGA AGCAGAACTG GCTGCGCATG AGCCGGCTAT TCAGGGTGTC 
CTGGACACTG GCAAGAAGCT GTCCGATGAC AACACCATCG GGAAAGAGGA GATCCAGCAG 
CGGCTGGCGC AGTTTGTGGA GCACTGGAAA GAGCTGAAGC AGCTGGCAGC TGCCCGGGGT 
CAGCGGCTGG AAGAGTCCTT GGAATATCAG CAGTTTGTAG CCAATGTGGA AGAGGAAGAA 
GCCTGGATCA ATGAGAAAAT GACCCTGGTG GCCAGCGAAG ATTATGGCGA CACTCTTGCC 
GCCATCCAGG GCTTACTGAA GAAACATGAA GCTTTTGAGA CAGACTTCAC CGTCCACAAG 
GATCGCGTGA ATGATGTCTG CACCAATGGA CAAGACCTCA TTAAGAAGAA CAATCACCAT 
GAGGAGAACA TCTCTTCAAA GATGAAGGGC CTGAACGGGA AAGTGTCAGA CCTGGAGAAA 
GCTGCAGCCC AGAGAAAGGC GAAGCTGGAT GAGAACTCGG CCTTCCTTCA GTTCAACTGG 
AAGGCGGACG TGGTGGAGTC CTGGATCGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT 
TATGGCCGAG ACCTGTCTTC TGTGCAGACG CTCCTCACCA AACAGGAAAC TTTTGACGCT 
GGGCTGCAGG CCTTCCAGCA GGAAGGCATT GCCAACATCA CTGCCCTCAA AGATCAGCTT 
CTCGCCGCCA AACACGTTCA GTCCAAGGCC ATCGAGGCCC GGCACGCCTC CCTCATGAAG 
AGGTGGAGCC AGCTTCTGGC CAACTCAGCC GCCCGCAAGA AGAAGCTTCT GGAGGCTCAG 
AGTCACTTCC GCAAGGTGGA GGACCTCTTC CTGACCTTCG CCAAAAAGGC TTCTGCCTTC 
AACAGCTGGT TTGAAAATGC AGAGGAGGAC TTAACAGACC CCGTGCGCTG CAACTCCTTG 
GAAGAAATCA AAGCTTTGCG CGAGGCCCAC GACGCCTTCC GCTCCTCCCT CAGCTCTGCC 
CAGGCTGACT TCAACCAGCT GGCCGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTAGCC 
TCCAACCCCT ACACCTGGTT TACCATGGAG GCCCTGGAGG AGACCTGGAG GAACCTACAG 
AAAATCATCA AGGAGAGGGA GCTGGAGCTG CAGAAGGAAC AGCGGCGGCA GGAGGAGAAC 
GACAAGCTGC GCCAGGAGTT TGCCCAGCAC GCCAACGCCT TCCACCAGTG GATCCAAGAG 
ACCAGGACAT ACCTCCTCGA TGGGTCCTGT ATGGTGGAAG AGTCGGGGAC CCTCGAATCC 
CAGCTTGAAG CTACCAAACG CAAGCACCAG GAAATCCGAG CCATGAGAAG TCAGCTCAAA 
AAGATCGAGG ACCTGGGGGC CGCCATGGAG GAGGCCCTCA TCCTGGACAA CAAGTACACG 
GAGCACAGCA CCGTGGGCCT CGCCCAGCAG TGGGACCAGC TGGACCAGCT GGGCATGCGC 
ATGCAGCACA ACCTGGAGCA GCAGATCCAG GCCAGGAACA CAACAGGTGT GACTGAGGAG 
GCCCTCAAAG AATTCAGCAT GATGTTTAAA CACTTTGACA AGGACAAGTC TGGCAGGCTG 
AACCATCAGG AGTTCAAATC TTGCCTGCGC TCCCTGGGCT ATGACCTGCC CATGGTGGAG 
GAAGGGGAAC CTGACCCTGA GTTCGAGGCA ATCCTGGACA CGGTGGATCC GAACAGAGAT 
GGCCATGTCT CCTTGCAAGA ATACATGGCT TTCATGATCA GCCGCGAAAC TGAGAACGTC 
AAGTCCAGCG AGGAGATTGA GAGCGCCTTC CGGGCCCTCA GCTCAGAGGG AAAGCCTTAC 
GTGACCAAGG AGGAGCTCTA CCAGAACCTG ACCCGGGAAC AAGCCGACTA CTGCGTCTCC 
CACATGAAGC CCTACGTGGA CGGCAAGGGC CGCGAGCTCC CCACCGCGTT CGACTACGTG 
GAGTTCACCC GCTCGCTTTT CGTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006717311.1
CDS	13..7482
Translation	MLSSFRKRRVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELASDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMRRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPTDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRITKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGMMPRDETDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKQKLDILDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAAGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINGRFQKIKSMAASRRAKLNESHRLHQFFRDMDDEESWIKEKKLLVGSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHASLMKRWSQLLANSAARKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_006717248.2
Organism	Homo sapiens(human)
Definition	Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006717248.2

ORF Insert Sequence:

ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACCG ATACCACCGC 
TTCAAGGAAC TCTCAACCCT TAGGCGTCAG AAGCTGGAAG ATTCCTATCG ATTCCAGTTC 
TTTCAAAGAG ATGCTGAAGA GCTGGAGAAA TGGATACAGG AAAAACTTCA GATTGCATCT 
GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGAAAGC TTCAGAAGCA TCAAGCATTT 
GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAGC TGGATGAAAC TGGAAACCTG 
ATGATCTCAG AAGGGCATTT TGCATCTGAA ACCATACGGA CCCGTTTGAT GGAGCTGCAC 
CGCCAGTGGG AATTACTTTT GGAGAAGATG CGAGAAAAAG GAATCAAACT GCTGCAGGCC 
CAGAAGTTGG TGCAGTACTT ACGAGAATGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCAATTG TTACTTCTGA AGAGCTGGGC CAGGATCTGG AGCATGTAGA GGTTTTACAG 
AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCTCATG AAGAAAGAGT TAATGAAGTG 
AACCAGTTTG CTGCCAAACT CATACAGGAG CAGCACCCTG AGGAGGAACT GATCAAGACT 
AAGCAGGATG AAGTCAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCTCT GCAGAGGCAG 
GGGAAGCTCT TTGGGGCAGC AGAAGTTCAG CGCTTTAACA GGGATGTGGA TGAGACTATC 
AGTTGGATTA AGGAAAAGGA GCAGTTAATG GCCTCTGATG ATTTTGGCCG AGACCTGGCA 
AGTGTTCAGG CTCTGCTTCG GAAGCACGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAA 
GACAAGGTCA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCCCA CCCTCTGAGT 
GCAACACAGA TTCAAGTGAA GCGAGAGGAA CTGATTACAA ACTGGGAGCA GATCCGCACC 
TTGGCGGCAG AGAGACATGC ACGGCTCAAT GATTCATACA GGCTTCAACG CTTCCTTGCT 
GACTTCCGTG ACCTCACCAG CTGGGTGACT GAGATGAAAG CCCTCATCAA TGCAGATGAG 
CTTGCCAGTG ATGTGGCTGG GGCTGAAGCC CTGCTAGATA GACACCAAGA GCACAAGGGT 
GAAATTGATG CCCATGAAGA CAGCTTCAAA TCTGCAGATG AATCTGGACA GGCACTGCTT 
GCTGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCGTCCT TTCCGAGGAG 
AGAGCGGCGC TGCTGGAGCT GTGGGAGCTG CGCAGGCAGC AGTACGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGGGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ATGAAGACTT GGGAGATTCC TTGGATAGTG TGGAAGCGCT TCTTAAGAAG 
CACGAAGACT TTGAGAAATC CCTTAGTGCC CAGGAGGAAA AGATTACAGC ATTAGATGAA 
TTTGCAACCA AGCTAATTCA GAACAACCAC TATGCAATGG AAGATGTGGC CACTCGCCGA 
GATGCTCTGT TGAGCCGCCG CAATGCCCTT CACGAGAGAG CCATGCGTCG CCGGGCCCAG 
CTAGCCGATT CTTTCCATCT GCAGCAGTTT TTCCGTGATT CTGATGAGCT CAAGAGTTGG 
GTCAATGAGA AGATGAAAAC TGCCACAGAT GAAGCTTATA AAGATCCATC CAACCTACAA 
GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CAGCAAACCA GAGCCGAATT 
GATGCCTTGG AGAAAGCTGG CCAAAAGCTG ATTGATGTCA ACCACTATGC CAAGGATGAA 
GTGGCAGCTC GTATGAATGA GGTGATCAGT TTGTGGAAGA AACTGCTAGA GGCCACTGAA 
CTGAAAGGAA TAAAGCTTCG TGAAGCCAAC CAGCAACAGC AATTTAATCG CAATGTTGAG 
GATATTGAAT TGTGGCTATA TGAAGTAGAA GGTCACTTGG CTTCGGATGA TTACGGCAAA 
GATCTTACCA ATGTGCAGAA CCTCCAGAAG AAACATGCAC TGCTAGAGGC AGATGTGGCT 
GCTCACCAGG ACCGAATTGA TGGCATCACC ATTCAGGCCC GCCAGTTCCA AGATGCTGGC 
CATTTTGATG CAGAAAACAT CAAGAAGAAA CAGGAAGCCC TCGTGGCTCG CTATGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCTCTGCG GTTGCAGCAG 
CTCTTCCGGG ATGTTGAGGA TGAGGAGACG TGGATTCGAG AGAAAGAGCC CATTGCCGCA 
TCTACCAACA GAGGTAAGGA TTTAATTGGG GTCCAGAATC TGCTAAAGAA ACATCAAGCC 
TTACAAGCAG AAATTGCTGG ACATGAACCA CGCATCAAAG CAGTTACACA GAAGGGGAAT 
GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAGCT TCACGAGCTG 
AACCAAAAGT GGGAGGCACT GAAAGCCAAA GCTTCCCAGC GTCGGCAGGA CCTGGAGGAC 
TCTCTGCAGG CCCAGCAGTA CTTTGCTGAT GCTAACGAGG CTGAATCCTG GATGCGGGAG 
AAGGAACCCA TTGTGGGCAG CACTGACTAT GGCAAGGACG AAGACTCTGC TGAGGCTCTA 
CTGAAGAAAC ACGAAGCTTT GATGTCAGAT CTCAGTGCCT ACGGCAGCAG CATCCAGGCT 
TTGCGAGAAC AAGCACAGTC CTGCCGGCAA CAAGTGGCCC CCACGGATGA TGAGACTGGG 
AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAGGGAG ATATCCTTAC CTTACTCAAC AGCACCAACA AGGATTGGTG GAAAGTGGAA 
GTGAACGATC GTCAGGGTTT TGTGCCGGCT GCGTACGTGA AGAAATTGGA CCCCGCCCAG 
TCAGCCTCCC GGGAGAATCT CCTGGAGGAG CAAGGCAGCA TAGCACTGCG GCAGGAGCAG 
ATTGACAATC AGACACGCAT AACTAAGGAG GCCGGCAGTG TATCTCTGCG TATGAAGCAG 
GTGGAAGAAC TATATCATTC TCTGCTGGAA CTGGGTGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGTTGCA AGAAGTTTAT GTTGTTCCGT GAAGCGAATG AACTACAGCA ATGGATCAAT 
GAGAAGGAAG CCGCTCTGAC AAGTGAGGAG GTCGGAGCAG ACTTGGAGCA GGTTGAGGTG 
CTCCAGAAGA AGTTTGATGA CTTCCAGAAG GACCTGAAGG CCAATGAGTC ACGGTTGAAG 
GACATTAACA AGGTAGCTGA AGACCTGGAG TCTGAAGGTC TCATGGCAGA GGAGGTGCAG 
GCTGTGCAAC AACAGGAAGT GTATGGCATG ATGCCCAGGG ATGAAACTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGTCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTGA ATGAGCGCTG GCGGTCCCTA CAGCAGCTGG CCGAGGAACG GAGCCAGCTC 
TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CTGATGAAAC CAAAGAATGG 
ATTGAAGAGA AGAATCAAGC TCTAAACACA GACAATTATG GACATGATCT CGCCAGTGTC 
CAGGCCCTGC AACGCAAGCA TGAGGGCTTC GAGAGGGACC TTGCGGCTCT CGGTGACAAG 
GTAAACTCCC TTGGTGAAAC AGCAGAGCGC CTGATCCAGT CCCATCCCGA GTCAGCAGAA 
GACCTGCAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA GCAGCCTGGG GAAACGTGCA 
GATCAGCGCA AGGCAAAGTT GGGTGACTCC CACGACCTGC AGCGCTTCCT TAGCGATTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATA CGGGGGTTGG TGTCCTCAGA TGAGCTAGCC 
AAGGATGTCA CCGGAGCTGA GGCATTGCTG GAGCGACACC AGGAACACCG GACAGAAATC 
GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GACAGCAGCT GTTGGCTCAC 
GGACACTATG CCAGCCCTGA GATCAAGCAG AAACTTGATA TTCTTGACCA GGAGCGTGCA 
GACCTGGAGA AGGCCTGGGT TCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAACTGCAG 
CTGTTCCATC GGGACTGTGA GCAAGCTGAG AACTGGATGG CTGCCCGGGA GGCCTTCTTG 
AATACCGAAG ACAAAGGAGA CTCACTGGAC AGCGTAGAGG CTCTGATCAA AAAACATGAA 
GACTTTGACA AAGCGATTAA CGTCCAGGAA GAGAAGATTG CTGCTCTGCA GGCCTTTGCC 
GACCAGCTCA TCGCTGCCGG CCATTATGCC AAGGGAGACA TTTCTAGCCG GCGCAATGAG 
GTCTTGGACA GGTGGCGACG TCTGAAAGCC CAGATGATTG AGAAAAGGTC AAAGCTAGGA 
GAATCTCAAA CCCTCCAACA GTTCAGCCGG GATGTGGATG AGATTGAGGC TTGGATCAGT 
GAAAAATTGC AAACAGCGAG TGATGAGTCG TACAAGGATC CCACCAACAT CCAGCTTTCC 
AAGCTGCTGA GCAAGCACCA GAAGCACCAG GCTTTTGAAG CAGAGCTGCA TGCCAACGCT 
GACCGGATCC GTGGGGTTAT CGACATGGGC AACTCCCTCA TTGAACGTGG AGCCTGTGCC 
GGCAGTGAGG ATGCTGTCAA GGCCCGCCTG GCTGCCTTAG CTGACCAGTG GCAGTTCTTG 
GTGCAAAAGT CAGCGGAAAA GAGCCAGAAA CTGAAAGAAG CCAACAAGCA GCAGAACTTC 
AACACAGGGA TCAAGGACTT TGACTTCTGG CTGTCTGAGG TGGAGGCCCT GCTGGCATCC 
GAAGATTATG GCAAAGACCT AGCTTCTGTG AACAACCTGC TGAAAAAGCA TCAACTGCTG 
GAAGCAGATA TATCTGCCCA TGAGGATCGC CTGAAGGACC TGAACAGCCA GGCAGACAGC 
CTGATGACCA GCAGTGCCTT CGACACCTCC CAAGTAAAGG ACAAGAGGGA CACCATCAAC 
GGGCGCTTCC AGAAGATCAA GAGCATGGCG GCCTCCCGGC GAGCCAAGCT GAATGAATCC 
CATCGCCTGC ACCAGTTCTT CCGGGACATG GATGACGAGG AGTCCTGGAT CAAGGAGAAG 
AAGCTGCTGG TGGGCTCAGA GGACTACGGC CGGGACCTAA CCGGCGTGCA GAACCTGAGG 
AAGAAGCACA AGCGGCTGGA AGCAGAACTG GCTGCGCATG AGCCGGCTAT TCAGGGTGTC 
CTGGACACTG GCAAGAAGCT GTCCGATGAC AACACCATCG GGAAAGAGGA GATCCAGCAG 
CGGCTGGCGC AGTTTGTGGA GCACTGGAAA GAGCTGAAGC AGCTGGCAGC TGCCCGGGGT 
CAGCGGCTGG AAGAGTCCTT GGAATATCAG CAGTTTGTAG CCAATGTGGA AGAGGAAGAA 
GCCTGGATCA ATGAGAAAAT GACCCTGGTG GCCAGCGAAG ATTATGGCGA CACTCTTGCC 
GCCATCCAGG GCTTACTGAA GAAACATGAA GCTTTTGAGA CAGACTTCAC CGTCCACAAG 
GATCGCGTGA ATGATGTCTG CACCAATGGA CAAGACCTCA TTAAGAAGAA CAATCACCAT 
GAGGAGAACA TCTCTTCAAA GATGAAGGGC CTGAACGGGA AAGTGTCAGA CCTGGAGAAA 
GCTGCAGCCC AGAGAAAGGC GAAGCTGGAT GAGAACTCGG CCTTCCTTCA GTTCAACTGG 
AAGGCGGACG TGGTGGAGTC CTGGATCGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT 
TATGGCCGAG ACCTGTCTTC TGTGCAGACG CTCCTCACCA AACAGGAAAC TTTTGACGCT 
GGGCTGCAGG CCTTCCAGCA GGAAGGCATT GCCAACATCA CTGCCCTCAA AGATCAGCTT 
CTCGCCGCCA AACACGTTCA GTCCAAGGCC ATCGAGGCCC GGCACGCCTC CCTCATGAAG 
AGGTGGAGCC AGCTTCTGGC CAACTCAGCC GCCCGCAAGA AGAAGCTTCT GGAGGCTCAG 
AGTCACTTCC GCAAGGTGGA GGACCTCTTC CTGACCTTCG CCAAAAAGGC TTCTGCCTTC 
AACAGCTGGT TTGAAAATGC AGAGGAGGAC TTAACAGACC CCGTGCGCTG CAACTCCTTG 
GAAGAAATCA AAGCTTTGCG CGAGGCCCAC GACGCCTTCC GCTCCTCCCT CAGCTCTGCC 
CAGGCTGACT TCAACCAGCT GGCCGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTAGCC 
TCCAACCCCT ACACCTGGTT TACCATGGAG GCCCTGGAGG AGACCTGGAG GAACCTACAG 
AAAATCATCA AGGAGAGGGA GCTGGAGCTG CAGAAGGAAC AGCGGCGGCA GGAGGAGAAC 
GACAAGCTGC GCCAGGAGTT TGCCCAGCAC GCCAACGCCT TCCACCAGTG GATCCAAGAG 
ACCAGGACAT ACCTCCTCGA TGGGTCCTGT ATGGTGGAAG AGTCGGGGAC CCTCGAATCC 
CAGCTTGAAG CTACCAAACG CAAGCACCAG GAAATCCGAG CCATGAGAAG TCAGCTCAAA 
AAGATCGAGG ACCTGGGGGC CGCCATGGAG GAGGCCCTCA TCCTGGACAA CAAGTACACG 
GAGCACAGCA CCGTGGGCCT CGCCCAGCAG TGGGACCAGC TGGACCAGCT GGGCATGCGC 
ATGCAGCACA ACCTGGAGCA GCAGATCCAG GCCAGGAACA CAACAGGTGT GACTGAGGAG 
GCCCTCAAAG AATTCAGCAT GATGTTTAAA CACTTTGACA AGGACAAGTC TGGCAGGCTG 
AACCATCAGG AGTTCAAATC TTGCCTGCGC TCCCTGGGCT ATGACCTGCC CATGGTGGAG 
GAAGGGGAAC CTGACCCTGA GTTCGAGGCA ATCCTGGACA CGGTGGATCC GAACAGAGAT 
GGCCATGTCT CCTTGCAAGA ATACATGGCT TTCATGATCA GCCGCGAAAC TGAGAACGTC 
AAGTCCAGCG AGGAGATTGA GAGCGCCTTC CGGGCCCTCA GCTCAGAGGG AAAGCCTTAC 
GTGACCAAGG AGGAGCTCTA CCAGAACCTG ACCCGGGAAC AAGCCGACTA CTGCGTCTCC 
CACATGAAGC CCTACGTGGA CGGCAAGGGC CGCGAGCTCC CCACCGCGTT CGACTACGTG 
GAGTTCACCC GCTCGCTTTT CGTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_006717249.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_006717312.1 Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X6, mRNA.

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CloneID	OHu43457
Clone ID Related Accession (Same CDS sequence)	XM_006717249.1
Accession Version	XM_006717249.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7455bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACCG ATACCACCGC 
TTCAAGGAAC TCTCAACCCT TAGGCGTCAG AAGCTGGAAG ATTCCTATCG ATTCCAGTTC 
TTTCAAAGAG ATGCTGAAGA GCTGGAGAAA TGGATACAGG AAAAACTTCA GATTGCATCT 
GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGAAAGC TTCAGAAGCA TCAAGCATTT 
GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAGC TGGATGAAAC TGGAAACCTG 
ATGATCTCAG AAGGGCATTT TGCATCTGAA ACCATACGGA CCCGTTTGAT GGAGCTGCAC 
CGCCAGTGGG AATTACTTTT GGAGAAGATG CGAGAAAAAG GAATCAAACT GCTGCAGGCC 
CAGAAGTTGG TGCAGTACTT ACGAGAATGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCAATTG TTACTTCTGA AGAGCTGGGC CAGGATCTGG AGCATGTAGA GGTTTTACAG 
AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCTCATG AAGAAAGAGT TAATGAAGTG 
AACCAGTTTG CTGCCAAACT CATACAGGAG CAGCACCCTG AGGAGGAACT GATCAAGACT 
AAGCAGGATG AAGTCAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCTCT GCAGAGGCAG 
GGGAAGCTCT TTGGGGCAGC AGAAGTTCAG CGCTTTAACA GGGATGTGGA TGAGACTATC 
AGTTGGATTA AGGAAAAGGA GCAGTTAATG GCCTCTGATG ATTTTGGCCG AGACCTGGCA 
AGTGTTCAGG CTCTGCTTCG GAAGCACGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAA 
GACAAGGTCA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCCCA CCCTCTGAGT 
GCAACACAGA TTCAAGTGAA GCGAGAGGAA CTGATTACAA ACTGGGAGCA GATCCGCACC 
TTGGCGGCAG AGAGACATGC ACGGCTCAAT GATTCATACA GGCTTCAACG CTTCCTTGCT 
GACTTCCGTG ACCTCACCAG CTGGGTGACT GAGATGAAAG CCCTCATCAA TGCAGATGAG 
CTTGCCAGTG ATGTGGCTGG GGCTGAAGCC CTGCTAGATA GACACCAAGA GCACAAGGGT 
GAAATTGATG CCCATGAAGA CAGCTTCAAA TCTGCAGATG AATCTGGACA GGCACTGCTT 
GCTGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCGTCCT TTCCGAGGAG 
AGAGCGGCGC TGCTGGAGCT GTGGGAGCTG CGCAGGCAGC AGTACGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGGGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ATGAAGACTT GGGAGATTCC TTGGATAGTG TGGAAGCGCT TCTTAAGAAG 
CACGAAGACT TTGAGAAATC CCTTAGTGCC CAGGAGGAAA AGATTACAGC ATTAGATGAA 
TTTGCAACCA AGCTAATTCA GAACAACCAC TATGCAATGG AAGATGTGGC CACTCGCCGA 
GATGCTCTGT TGAGCCGCCG CAATGCCCTT CACGAGAGAG CCATGCGTCG CCGGGCCCAG 
CTAGCCGATT CTTTCCATCT GCAGCAGTTT TTCCGTGATT CTGATGAGCT CAAGAGTTGG 
GTCAATGAGA AGATGAAAAC TGCCACAGAT GAAGCTTATA AAGATCCATC CAACCTACAA 
GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CAGCAAACCA GAGCCGAATT 
GATGCCTTGG AGAAAGCTGG CCAAAAGCTG ATTGATGTCA ACCACTATGC CAAGGATGAA 
GTGGCAGCTC GTATGAATGA GGTGATCAGT TTGTGGAAGA AACTGCTAGA GGCCACTGAA 
CTGAAAGGAA TAAAGCTTCG TGAAGCCAAC CAGCAACAGC AATTTAATCG CAATGTTGAG 
GATATTGAAT TGTGGCTATA TGAAGTAGAA GGTCACTTGG CTTCGGATGA TTACGGCAAA 
GATCTTACCA ATGTGCAGAA CCTCCAGAAG AAACATGCAC TGCTAGAGGC AGATGTGGCT 
GCTCACCAGG ACCGAATTGA TGGCATCACC ATTCAGGCCC GCCAGTTCCA AGATGCTGGC 
CATTTTGATG CAGAAAACAT CAAGAAGAAA CAGGAAGCCC TCGTGGCTCG CTATGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCTCTGCG GTTGCAGCAG 
CTCTTCCGGG ATGTTGAGGA TGAGGAGACG TGGATTCGAG AGAAAGAGCC CATTGCCGCA 
TCTACCAACA GAGGTAAGGA TTTAATTGGG GTCCAGAATC TGCTAAAGAA ACATCAAGCC 
TTACAAGCAG AAATTGCTGG ACATGAACCA CGCATCAAAG CAGTTACACA GAAGGGGAAT 
GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAGCT TCACGAGCTG 
AACCAAAAGT GGGAGGCACT GAAAGCCAAA GCTTCCCAGC GTCGGCAGGA CCTGGAGGAC 
TCTCTGCAGG CCCAGCAGTA CTTTGCTGAT GCTAACGAGG CTGAATCCTG GATGCGGGAG 
AAGGAACCCA TTGTGGGCAG CACTGACTAT GGCAAGGACG AAGACTCTGC TGAGGCTCTA 
CTGAAGAAAC ACGAAGCTTT GATGTCAGAT CTCAGTGCCT ACGGCAGCAG CATCCAGGCT 
TTGCGAGAAC AAGCACAGTC CTGCCGGCAA CAAGTGGCCC CCACGGATGA TGAGACTGGG 
AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAGGGAG ATATCCTTAC CTTACTCAAC AGCACCAACA AGGATTGGTG GAAAGTGGAA 
GTGAACGATC GTCAGGGTTT TGTGCCGGCT GCGTACGTGA AGAAATTGGA CCCCGCCCAG 
TCAGCCTCCC GGGAGAATCT CCTGGAGGAG CAAGGCAGCA TAGCACTGCG GCAGGAGCAG 
ATTGACAATC AGACACGCAT AACTAAGGAG GCCGGCAGTG TATCTCTGCG TATGAAGCAG 
GTGGAAGAAC TATATCATTC TCTGCTGGAA CTGGGTGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGTTGCA AGAAGTTTAT GTTGTTCCGT GAAGCGAATG AACTACAGCA ATGGATCAAT 
GAGAAGGAAG CCGCTCTGAC AAGTGAGGAG GTCGGAGCAG ACTTGGAGCA GGTTGAGGTG 
CTCCAGAAGA AGTTTGATGA CTTCCAGAAG GACCTGAAGG CCAATGAGTC ACGGTTGAAG 
GACATTAACA AGGTAGCTGA AGACCTGGAG TCTGAAGGTC TCATGGCAGA GGAGGTGCAG 
GCTGTGCAAC AACAGGAAGT GTATGGCATG ATGCCCAGGG ATGAAACTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGTCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTGA ATGAGCGCTG GCGGTCCCTA CAGCAGCTGG CCGAGGAACG GAGCCAGCTC 
TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CTGATGAAAC CAAAGAATGG 
ATTGAAGAGA AGAATCAAGC TCTAAACACA GACAATTATG GACATGATCT CGCCAGTGTC 
CAGGCCCTGC AACGCAAGCA TGAGGGCTTC GAGAGGGACC TTGCGGCTCT CGGTGACAAG 
GTAAACTCCC TTGGTGAAAC AGCAGAGCGC CTGATCCAGT CCCATCCCGA GTCAGCAGAA 
GACCTGCAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA GCAGCCTGGG GAAACGTGCA 
GATCAGCGCA AGGCAAAGTT GGGTGACTCC CACGACCTGC AGCGCTTCCT TAGCGATTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATA CGGGGGTTGG TGTCCTCAGA TGAGCTAGCC 
AAGGATGTCA CCGGAGCTGA GGCATTGCTG GAGCGACACC AGGAACACCG GACAGAAATC 
GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GACAGCAGCT GTTGGCTCAC 
GGACACTATG CCAGCCCTGA GATCAAGCAG AAACTTGATA TTCTTGACCA GGAGCGTGCA 
GACCTGGAGA AGGCCTGGGT TCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAACTGCAG 
CTGTTCCATC GGGACTGTGA GCAAGCTGAG AACTGGATGG CTGCCCGGGA GGCCTTCTTG 
AATACCGAAG ACAAAGGAGA CTCACTGGAC AGCGTAGAGG CTCTGATCAA AAAACATGAA 
GACTTTGACA AAGCGATTAA CGTCCAGGAA GAGAAGATTG CTGCTCTGCA GGCCTTTGCC 
GACCAGCTCA TCGCTGCCGG CCATTATGCC AAGGGAGACA TTTCTAGCCG GCGCAATGAG 
GTCTTGGACA GGTGGCGACG TCTGAAAGCC CAGATGATTG AGAAAAGGTC AAAGCTAGGA 
GAATCTCAAA CCCTCCAACA GTTCAGCCGG GATGTGGATG AGATTGAGGC TTGGATCAGT 
GAAAAATTGC AAACAGCGAG TGATGAGTCG TACAAGGATC CCACCAACAT CCAGAGCAAG 
CACCAGAAGC ACCAGGCTTT TGAAGCAGAG CTGCATGCCA ACGCTGACCG GATCCGTGGG 
GTTATCGACA TGGGCAACTC CCTCATTGAA CGTGGAGCCT GTGCCGGCAG TGAGGATGCT 
GTCAAGGCCC GCCTGGCTGC CTTAGCTGAC CAGTGGCAGT TCTTGGTGCA AAAGTCAGCG 
GAAAAGAGCC AGAAACTGAA AGAAGCCAAC AAGCAGCAGA ACTTCAACAC AGGGATCAAG 
GACTTTGACT TCTGGCTGTC TGAGGTGGAG GCCCTGCTGG CATCCGAAGA TTATGGCAAA 
GACCTAGCTT CTGTGAACAA CCTGCTGAAA AAGCATCAAC TGCTGGAAGC AGATATATCT 
GCCCATGAGG ATCGCCTGAA GGACCTGAAC AGCCAGGCAG ACAGCCTGAT GACCAGCAGT 
GCCTTCGACA CCTCCCAAGT AAAGGACAAG AGGGACACCA TCAACGGGCG CTTCCAGAAG 
ATCAAGAGCA TGGCGGCCTC CCGGCGAGCC AAGCTGAATG AATCCCATCG CCTGCACCAG 
TTCTTCCGGG ACATGGATGA CGAGGAGTCC TGGATCAAGG AGAAGAAGCT GCTGGTGGGC 
TCAGAGGACT ACGGCCGGGA CCTAACCGGC GTGCAGAACC TGAGGAAGAA GCACAAGCGG 
CTGGAAGCAG AACTGGCTGC GCATGAGCCG GCTATTCAGG GTGTCCTGGA CACTGGCAAG 
AAGCTGTCCG ATGACAACAC CATCGGGAAA GAGGAGATCC AGCAGCGGCT GGCGCAGTTT 
GTGGAGCACT GGAAAGAGCT GAAGCAGCTG GCAGCTGCCC GGGGTCAGCG GCTGGAAGAG 
TCCTTGGAAT ATCAGCAGTT TGTAGCCAAT GTGGAAGAGG AAGAAGCCTG GATCAATGAG 
AAAATGACCC TGGTGGCCAG CGAAGATTAT GGCGACACTC TTGCCGCCAT CCAGGGCTTA 
CTGAAGAAAC ATGAAGCTTT TGAGACAGAC TTCACCGTCC ACAAGGATCG CGTGAATGAT 
GTCTGCACCA ATGGACAAGA CCTCATTAAG AAGAACAATC ACCATGAGGA GAACATCTCT 
TCAAAGATGA AGGGCCTGAA CGGGAAAGTG TCAGACCTGG AGAAAGCTGC AGCCCAGAGA 
AAGGCGAAGC TGGATGAGAA CTCGGCCTTC CTTCAGTTCA ACTGGAAGGC GGACGTGGTG 
GAGTCCTGGA TCGGTGAAAA GGAGAACAGC TTGAAGACAG ATGATTATGG CCGAGACCTG 
TCTTCTGTGC AGACGCTCCT CACCAAACAG GAAACTTTTG ACGCTGGGCT GCAGGCCTTC 
CAGCAGGAAG GCATTGCCAA CATCACTGCC CTCAAAGATC AGCTTCTCGC CGCCAAACAC 
GTTCAGTCCA AGGCCATCGA GGCCCGGCAC GCCTCCCTCA TGAAGAGGTG GAGCCAGCTT 
CTGGCCAACT CAGCCGCCCG CAAGAAGAAG CTTCTGGAGG CTCAGAGTCA CTTCCGCAAG 
GTGGAGGACC TCTTCCTGAC CTTCGCCAAA AAGGCTTCTG CCTTCAACAG CTGGTTTGAA 
AATGCAGAGG AGGACTTAAC AGACCCCGTG CGCTGCAACT CCTTGGAAGA AATCAAAGCT 
TTGCGCGAGG CCCACGACGC CTTCCGCTCC TCCCTCAGCT CTGCCCAGGC TGACTTCAAC 
CAGCTGGCCG AGCTGGACCG CCAGATCAAG AGCTTCCGCG TAGCCTCCAA CCCCTACACC 
TGGTTTACCA TGGAGGCCCT GGAGGAGACC TGGAGGAACC TACAGAAAAT CATCAAGGAG 
AGGGAGCTGG AGCTGCAGAA GGAACAGCGG CGGCAGGAGG AGAACGACAA GCTGCGCCAG 
GAGTTTGCCC AGCACGCCAA CGCCTTCCAC CAGTGGATCC AAGAGACCAG GACATACCTC 
CTCGATGGGT CCTGTATGGT GGAAGAGTCG GGGACCCTCG AATCCCAGCT TGAAGCTACC 
AAACGCAAGC ACCAGGAAAT CCGAGCCATG AGAAGTCAGC TCAAAAAGAT CGAGGACCTG 
GGGGCCGCCA TGGAGGAGGC CCTCATCCTG GACAACAAGT ACACGGAGCA CAGCACCGTG 
GGCCTCGCCC AGCAGTGGGA CCAGCTGGAC CAGCTGGGCA TGCGCATGCA GCACAACCTG 
GAGCAGCAGA TCCAGGCCAG GAACACAACA GGTGTGACTG AGGAGGCCCT CAAAGAATTC 
AGCATGATGT TTAAACACTT TGACAAGGAC AAGTCTGGCA GGCTGAACCA TCAGGAGTTC 
AAATCTTGCC TGCGCTCCCT GGGCTATGAC CTGCCCATGG TGGAGGAAGG GGAACCTGAC 
CCTGAGTTCG AGGCAATCCT GGACACGGTG GATCCGAACA GAGATGGCCA TGTCTCCTTG 
CAAGAATACA TGGCTTTCAT GATCAGCCGC GAAACTGAGA ACGTCAAGTC CAGCGAGGAG 
ATTGAGAGCG CCTTCCGGGC CCTCAGCTCA GAGGGAAAGC CTTACGTGAC CAAGGAGGAG 
CTCTACCAGA ACCTGACCCG GGAACAAGCC GACTACTGCG TCTCCCACAT GAAGCCCTAC 
GTGGACGGCA AGGGCCGCGA GCTCCCCACC GCGTTCGACT ACGTGGAGTT CACCCGCTCG 
CTTTTCGTGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006717312.1
CDS	14..7468
Translation	MLSSFRKRRVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELASDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMRRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPTDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRITKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGMMPRDETDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKQKLDILDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAAGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINGRFQKIKSMAASRRAKLNESHRLHQFFRDMDDEESWIKEKKLLVGSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHASLMKRWSQLLANSAARKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_006717249.1
Organism	Homo sapiens(human)
Definition	Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006717249.1

ORF Insert Sequence:

ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACCG ATACCACCGC 
TTCAAGGAAC TCTCAACCCT TAGGCGTCAG AAGCTGGAAG ATTCCTATCG ATTCCAGTTC 
TTTCAAAGAG ATGCTGAAGA GCTGGAGAAA TGGATACAGG AAAAACTTCA GATTGCATCT 
GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGAAAGC TTCAGAAGCA TCAAGCATTT 
GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAGC TGGATGAAAC TGGAAACCTG 
ATGATCTCAG AAGGGCATTT TGCATCTGAA ACCATACGGA CCCGTTTGAT GGAGCTGCAC 
CGCCAGTGGG AATTACTTTT GGAGAAGATG CGAGAAAAAG GAATCAAACT GCTGCAGGCC 
CAGAAGTTGG TGCAGTACTT ACGAGAATGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCAATTG TTACTTCTGA AGAGCTGGGC CAGGATCTGG AGCATGTAGA GGTTTTACAG 
AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCTCATG AAGAAAGAGT TAATGAAGTG 
AACCAGTTTG CTGCCAAACT CATACAGGAG CAGCACCCTG AGGAGGAACT GATCAAGACT 
AAGCAGGATG AAGTCAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCTCT GCAGAGGCAG 
GGGAAGCTCT TTGGGGCAGC AGAAGTTCAG CGCTTTAACA GGGATGTGGA TGAGACTATC 
AGTTGGATTA AGGAAAAGGA GCAGTTAATG GCCTCTGATG ATTTTGGCCG AGACCTGGCA 
AGTGTTCAGG CTCTGCTTCG GAAGCACGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAA 
GACAAGGTCA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCCCA CCCTCTGAGT 
GCAACACAGA TTCAAGTGAA GCGAGAGGAA CTGATTACAA ACTGGGAGCA GATCCGCACC 
TTGGCGGCAG AGAGACATGC ACGGCTCAAT GATTCATACA GGCTTCAACG CTTCCTTGCT 
GACTTCCGTG ACCTCACCAG CTGGGTGACT GAGATGAAAG CCCTCATCAA TGCAGATGAG 
CTTGCCAGTG ATGTGGCTGG GGCTGAAGCC CTGCTAGATA GACACCAAGA GCACAAGGGT 
GAAATTGATG CCCATGAAGA CAGCTTCAAA TCTGCAGATG AATCTGGACA GGCACTGCTT 
GCTGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCGTCCT TTCCGAGGAG 
AGAGCGGCGC TGCTGGAGCT GTGGGAGCTG CGCAGGCAGC AGTACGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGGGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ATGAAGACTT GGGAGATTCC TTGGATAGTG TGGAAGCGCT TCTTAAGAAG 
CACGAAGACT TTGAGAAATC CCTTAGTGCC CAGGAGGAAA AGATTACAGC ATTAGATGAA 
TTTGCAACCA AGCTAATTCA GAACAACCAC TATGCAATGG AAGATGTGGC CACTCGCCGA 
GATGCTCTGT TGAGCCGCCG CAATGCCCTT CACGAGAGAG CCATGCGTCG CCGGGCCCAG 
CTAGCCGATT CTTTCCATCT GCAGCAGTTT TTCCGTGATT CTGATGAGCT CAAGAGTTGG 
GTCAATGAGA AGATGAAAAC TGCCACAGAT GAAGCTTATA AAGATCCATC CAACCTACAA 
GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CAGCAAACCA GAGCCGAATT 
GATGCCTTGG AGAAAGCTGG CCAAAAGCTG ATTGATGTCA ACCACTATGC CAAGGATGAA 
GTGGCAGCTC GTATGAATGA GGTGATCAGT TTGTGGAAGA AACTGCTAGA GGCCACTGAA 
CTGAAAGGAA TAAAGCTTCG TGAAGCCAAC CAGCAACAGC AATTTAATCG CAATGTTGAG 
GATATTGAAT TGTGGCTATA TGAAGTAGAA GGTCACTTGG CTTCGGATGA TTACGGCAAA 
GATCTTACCA ATGTGCAGAA CCTCCAGAAG AAACATGCAC TGCTAGAGGC AGATGTGGCT 
GCTCACCAGG ACCGAATTGA TGGCATCACC ATTCAGGCCC GCCAGTTCCA AGATGCTGGC 
CATTTTGATG CAGAAAACAT CAAGAAGAAA CAGGAAGCCC TCGTGGCTCG CTATGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCTCTGCG GTTGCAGCAG 
CTCTTCCGGG ATGTTGAGGA TGAGGAGACG TGGATTCGAG AGAAAGAGCC CATTGCCGCA 
TCTACCAACA GAGGTAAGGA TTTAATTGGG GTCCAGAATC TGCTAAAGAA ACATCAAGCC 
TTACAAGCAG AAATTGCTGG ACATGAACCA CGCATCAAAG CAGTTACACA GAAGGGGAAT 
GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAGCT TCACGAGCTG 
AACCAAAAGT GGGAGGCACT GAAAGCCAAA GCTTCCCAGC GTCGGCAGGA CCTGGAGGAC 
TCTCTGCAGG CCCAGCAGTA CTTTGCTGAT GCTAACGAGG CTGAATCCTG GATGCGGGAG 
AAGGAACCCA TTGTGGGCAG CACTGACTAT GGCAAGGACG AAGACTCTGC TGAGGCTCTA 
CTGAAGAAAC ACGAAGCTTT GATGTCAGAT CTCAGTGCCT ACGGCAGCAG CATCCAGGCT 
TTGCGAGAAC AAGCACAGTC CTGCCGGCAA CAAGTGGCCC CCACGGATGA TGAGACTGGG 
AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAGGGAG ATATCCTTAC CTTACTCAAC AGCACCAACA AGGATTGGTG GAAAGTGGAA 
GTGAACGATC GTCAGGGTTT TGTGCCGGCT GCGTACGTGA AGAAATTGGA CCCCGCCCAG 
TCAGCCTCCC GGGAGAATCT CCTGGAGGAG CAAGGCAGCA TAGCACTGCG GCAGGAGCAG 
ATTGACAATC AGACACGCAT AACTAAGGAG GCCGGCAGTG TATCTCTGCG TATGAAGCAG 
GTGGAAGAAC TATATCATTC TCTGCTGGAA CTGGGTGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGTTGCA AGAAGTTTAT GTTGTTCCGT GAAGCGAATG AACTACAGCA ATGGATCAAT 
GAGAAGGAAG CCGCTCTGAC AAGTGAGGAG GTCGGAGCAG ACTTGGAGCA GGTTGAGGTG 
CTCCAGAAGA AGTTTGATGA CTTCCAGAAG GACCTGAAGG CCAATGAGTC ACGGTTGAAG 
GACATTAACA AGGTAGCTGA AGACCTGGAG TCTGAAGGTC TCATGGCAGA GGAGGTGCAG 
GCTGTGCAAC AACAGGAAGT GTATGGCATG ATGCCCAGGG ATGAAACTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGTCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTGA ATGAGCGCTG GCGGTCCCTA CAGCAGCTGG CCGAGGAACG GAGCCAGCTC 
TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CTGATGAAAC CAAAGAATGG 
ATTGAAGAGA AGAATCAAGC TCTAAACACA GACAATTATG GACATGATCT CGCCAGTGTC 
CAGGCCCTGC AACGCAAGCA TGAGGGCTTC GAGAGGGACC TTGCGGCTCT CGGTGACAAG 
GTAAACTCCC TTGGTGAAAC AGCAGAGCGC CTGATCCAGT CCCATCCCGA GTCAGCAGAA 
GACCTGCAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA GCAGCCTGGG GAAACGTGCA 
GATCAGCGCA AGGCAAAGTT GGGTGACTCC CACGACCTGC AGCGCTTCCT TAGCGATTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATA CGGGGGTTGG TGTCCTCAGA TGAGCTAGCC 
AAGGATGTCA CCGGAGCTGA GGCATTGCTG GAGCGACACC AGGAACACCG GACAGAAATC 
GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GACAGCAGCT GTTGGCTCAC 
GGACACTATG CCAGCCCTGA GATCAAGCAG AAACTTGATA TTCTTGACCA GGAGCGTGCA 
GACCTGGAGA AGGCCTGGGT TCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAACTGCAG 
CTGTTCCATC GGGACTGTGA GCAAGCTGAG AACTGGATGG CTGCCCGGGA GGCCTTCTTG 
AATACCGAAG ACAAAGGAGA CTCACTGGAC AGCGTAGAGG CTCTGATCAA AAAACATGAA 
GACTTTGACA AAGCGATTAA CGTCCAGGAA GAGAAGATTG CTGCTCTGCA GGCCTTTGCC 
GACCAGCTCA TCGCTGCCGG CCATTATGCC AAGGGAGACA TTTCTAGCCG GCGCAATGAG 
GTCTTGGACA GGTGGCGACG TCTGAAAGCC CAGATGATTG AGAAAAGGTC AAAGCTAGGA 
GAATCTCAAA CCCTCCAACA GTTCAGCCGG GATGTGGATG AGATTGAGGC TTGGATCAGT 
GAAAAATTGC AAACAGCGAG TGATGAGTCG TACAAGGATC CCACCAACAT CCAGAGCAAG 
CACCAGAAGC ACCAGGCTTT TGAAGCAGAG CTGCATGCCA ACGCTGACCG GATCCGTGGG 
GTTATCGACA TGGGCAACTC CCTCATTGAA CGTGGAGCCT GTGCCGGCAG TGAGGATGCT 
GTCAAGGCCC GCCTGGCTGC CTTAGCTGAC CAGTGGCAGT TCTTGGTGCA AAAGTCAGCG 
GAAAAGAGCC AGAAACTGAA AGAAGCCAAC AAGCAGCAGA ACTTCAACAC AGGGATCAAG 
GACTTTGACT TCTGGCTGTC TGAGGTGGAG GCCCTGCTGG CATCCGAAGA TTATGGCAAA 
GACCTAGCTT CTGTGAACAA CCTGCTGAAA AAGCATCAAC TGCTGGAAGC AGATATATCT 
GCCCATGAGG ATCGCCTGAA GGACCTGAAC AGCCAGGCAG ACAGCCTGAT GACCAGCAGT 
GCCTTCGACA CCTCCCAAGT AAAGGACAAG AGGGACACCA TCAACGGGCG CTTCCAGAAG 
ATCAAGAGCA TGGCGGCCTC CCGGCGAGCC AAGCTGAATG AATCCCATCG CCTGCACCAG 
TTCTTCCGGG ACATGGATGA CGAGGAGTCC TGGATCAAGG AGAAGAAGCT GCTGGTGGGC 
TCAGAGGACT ACGGCCGGGA CCTAACCGGC GTGCAGAACC TGAGGAAGAA GCACAAGCGG 
CTGGAAGCAG AACTGGCTGC GCATGAGCCG GCTATTCAGG GTGTCCTGGA CACTGGCAAG 
AAGCTGTCCG ATGACAACAC CATCGGGAAA GAGGAGATCC AGCAGCGGCT GGCGCAGTTT 
GTGGAGCACT GGAAAGAGCT GAAGCAGCTG GCAGCTGCCC GGGGTCAGCG GCTGGAAGAG 
TCCTTGGAAT ATCAGCAGTT TGTAGCCAAT GTGGAAGAGG AAGAAGCCTG GATCAATGAG 
AAAATGACCC TGGTGGCCAG CGAAGATTAT GGCGACACTC TTGCCGCCAT CCAGGGCTTA 
CTGAAGAAAC ATGAAGCTTT TGAGACAGAC TTCACCGTCC ACAAGGATCG CGTGAATGAT 
GTCTGCACCA ATGGACAAGA CCTCATTAAG AAGAACAATC ACCATGAGGA GAACATCTCT 
TCAAAGATGA AGGGCCTGAA CGGGAAAGTG TCAGACCTGG AGAAAGCTGC AGCCCAGAGA 
AAGGCGAAGC TGGATGAGAA CTCGGCCTTC CTTCAGTTCA ACTGGAAGGC GGACGTGGTG 
GAGTCCTGGA TCGGTGAAAA GGAGAACAGC TTGAAGACAG ATGATTATGG CCGAGACCTG 
TCTTCTGTGC AGACGCTCCT CACCAAACAG GAAACTTTTG ACGCTGGGCT GCAGGCCTTC 
CAGCAGGAAG GCATTGCCAA CATCACTGCC CTCAAAGATC AGCTTCTCGC CGCCAAACAC 
GTTCAGTCCA AGGCCATCGA GGCCCGGCAC GCCTCCCTCA TGAAGAGGTG GAGCCAGCTT 
CTGGCCAACT CAGCCGCCCG CAAGAAGAAG CTTCTGGAGG CTCAGAGTCA CTTCCGCAAG 
GTGGAGGACC TCTTCCTGAC CTTCGCCAAA AAGGCTTCTG CCTTCAACAG CTGGTTTGAA 
AATGCAGAGG AGGACTTAAC AGACCCCGTG CGCTGCAACT CCTTGGAAGA AATCAAAGCT 
TTGCGCGAGG CCCACGACGC CTTCCGCTCC TCCCTCAGCT CTGCCCAGGC TGACTTCAAC 
CAGCTGGCCG AGCTGGACCG CCAGATCAAG AGCTTCCGCG TAGCCTCCAA CCCCTACACC 
TGGTTTACCA TGGAGGCCCT GGAGGAGACC TGGAGGAACC TACAGAAAAT CATCAAGGAG 
AGGGAGCTGG AGCTGCAGAA GGAACAGCGG CGGCAGGAGG AGAACGACAA GCTGCGCCAG 
GAGTTTGCCC AGCACGCCAA CGCCTTCCAC CAGTGGATCC AAGAGACCAG GACATACCTC 
CTCGATGGGT CCTGTATGGT GGAAGAGTCG GGGACCCTCG AATCCCAGCT TGAAGCTACC 
AAACGCAAGC ACCAGGAAAT CCGAGCCATG AGAAGTCAGC TCAAAAAGAT CGAGGACCTG 
GGGGCCGCCA TGGAGGAGGC CCTCATCCTG GACAACAAGT ACACGGAGCA CAGCACCGTG 
GGCCTCGCCC AGCAGTGGGA CCAGCTGGAC CAGCTGGGCA TGCGCATGCA GCACAACCTG 
GAGCAGCAGA TCCAGGCCAG GAACACAACA GGTGTGACTG AGGAGGCCCT CAAAGAATTC 
AGCATGATGT TTAAACACTT TGACAAGGAC AAGTCTGGCA GGCTGAACCA TCAGGAGTTC 
AAATCTTGCC TGCGCTCCCT GGGCTATGAC CTGCCCATGG TGGAGGAAGG GGAACCTGAC 
CCTGAGTTCG AGGCAATCCT GGACACGGTG GATCCGAACA GAGATGGCCA TGTCTCCTTG 
CAAGAATACA TGGCTTTCAT GATCAGCCGC GAAACTGAGA ACGTCAAGTC CAGCGAGGAG 
ATTGAGAGCG CCTTCCGGGC CCTCAGCTCA GAGGGAAAGC CTTACGTGAC CAAGGAGGAG 
CTCTACCAGA ACCTGACCCG GGAACAAGCC GACTACTGCG TCTCCCACAT GAAGCCCTAC 
GTGGACGGCA AGGGCCGCGA GCTCCCCACC GCGTTCGACT ACGTGGAGTT CACCCGCTCG 
CTTTTCGTGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_006717250.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_006717313.1 Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X7, mRNA.

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CloneID	OHu43458
Clone ID Related Accession (Same CDS sequence)	XM_006717250.1
Accession Version	XM_006717250.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7452bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACCG ATACCACCGC 
TTCAAGGAAC TCTCAACCCT TAGGCGTCAG AAGCTGGAAG ATTCCTATCG ATTCCAGTTC 
TTTCAAAGAG ATGCTGAAGA GCTGGAGAAA TGGATACAGG AAAAACTTCA GATTGCATCT 
GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGAAAGC TTCAGAAGCA TCAAGCATTT 
GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAGC TGGATGAAAC TGGAAACCTG 
ATGATCTCAG AAGGGCATTT TGCATCTGAA ACCATACGGA CCCGTTTGAT GGAGCTGCAC 
CGCCAGTGGG AATTACTTTT GGAGAAGATG CGAGAAAAAG GAATCAAACT GCTGCAGGCC 
CAGAAGTTGG TGCAGTACTT ACGAGAATGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCAATTG TTACTTCTGA AGAGCTGGGC CAGGATCTGG AGCATGTAGA GGTTTTACAG 
AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCTCATG AAGAAAGAGT TAATGAAGTG 
AACCAGTTTG CTGCCAAACT CATACAGGAG CAGCACCCTG AGGAGGAACT GATCAAGACT 
AAGCAGGATG AAGTCAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCTCT GCAGAGGCAG 
GGGAAGCTCT TTGGGGCAGC AGAAGTTCAG CGCTTTAACA GGGATGTGGA TGAGACTATC 
AGTTGGATTA AGGAAAAGGA GCAGTTAATG GCCTCTGATG ATTTTGGCCG AGACCTGGCA 
AGTGTTCAGG CTCTGCTTCG GAAGCACGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAA 
GACAAGGTCA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCCCA CCCTCTGAGT 
GCAACACAGA TTCAAGTGAA GCGAGAGGAA CTGATTACAA ACTGGGAGCA GATCCGCACC 
TTGGCGGCAG AGAGACATGC ACGGCTCAAT GATTCATACA GGCTTCAACG CTTCCTTGCT 
GACTTCCGTG ACCTCACCAG CTGGGTGACT GAGATGAAAG CCCTCATCAA TGCAGATGAG 
CTTGCCAGTG ATGTGGCTGG GGCTGAAGCC CTGCTAGATA GACACCAAGA GCACAAGGGT 
GAAATTGATG CCCATGAAGA CAGCTTCAAA TCTGCAGATG AATCTGGACA GGCACTGCTT 
GCTGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCGTCCT TTCCGAGGAG 
AGAGCGGCGC TGCTGGAGCT GTGGGAGCTG CGCAGGCAGC AGTACGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGGGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ATGAAGACTT GGGAGATTCC TTGGATAGTG TGGAAGCGCT TCTTAAGAAG 
CACGAAGACT TTGAGAAATC CCTTAGTGCC CAGGAGGAAA AGATTACAGC ATTAGATGAA 
TTTGCAACCA AGCTAATTCA GAACAACCAC TATGCAATGG AAGATGTGGC CACTCGCCGA 
GATGCTCTGT TGAGCCGCCG CAATGCCCTT CACGAGAGAG CCATGCGTCG CCGGGCCCAG 
CTAGCCGATT CTTTCCATCT GCAGCAGTTT TTCCGTGATT CTGATGAGCT CAAGAGTTGG 
GTCAATGAGA AGATGAAAAC TGCCACAGAT GAAGCTTATA AAGATCCATC CAACCTACAA 
GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CAGCAAACCA GAGCCGAATT 
GATGCCTTGG AGAAAGCTGG CCAAAAGCTG ATTGATGTCA ACCACTATGC CAAGGATGAA 
GTGGCAGCTC GTATGAATGA GGTGATCAGT TTGTGGAAGA AACTGCTAGA GGCCACTGAA 
CTGAAAGGAA TAAAGCTTCG TGAAGCCAAC CAGCAACAGC AATTTAATCG CAATGTTGAG 
GATATTGAAT TGTGGCTATA TGAAGTAGAA GGTCACTTGG CTTCGGATGA TTACGGCAAA 
GATCTTACCA ATGTGCAGAA CCTCCAGAAG AAACATGCAC TGCTAGAGGC AGATGTGGCT 
GCTCACCAGG ACCGAATTGA TGGCATCACC ATTCAGGCCC GCCAGTTCCA AGATGCTGGC 
CATTTTGATG CAGAAAACAT CAAGAAGAAA CAGGAAGCCC TCGTGGCTCG CTATGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCTCTGCG GTTGCAGCAG 
CTCTTCCGGG ATGTTGAGGA TGAGGAGACG TGGATTCGAG AGAAAGAGCC CATTGCCGCA 
TCTACCAACA GAGGTAAGGA TTTAATTGGG GTCCAGAATC TGCTAAAGAA ACATCAAGCC 
TTACAAGCAG AAATTGCTGG ACATGAACCA CGCATCAAAG CAGTTACACA GAAGGGGAAT 
GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAGCT TCACGAGCTG 
AACCAAAAGT GGGAGGCACT GAAAGCCAAA GCTTCCCAGC GTCGGCAGGA CCTGGAGGAC 
TCTCTGCAGG CCCAGCAGTA CTTTGCTGAT GCTAACGAGG CTGAATCCTG GATGCGGGAG 
AAGGAACCCA TTGTGGGCAG CACTGACTAT GGCAAGGACG AAGACTCTGC TGAGGCTCTA 
CTGAAGAAAC ACGAAGCTTT GATGTCAGAT CTCAGTGCCT ACGGCAGCAG CATCCAGGCT 
TTGCGAGAAC AAGCACAGTC CTGCCGGCAA CAAGTGGCCC CCACGGATGA TGAGACTGGG 
AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAGGGAG ATATCCTTAC CTTACTCAAC AGCACCAACA AGGATTGGTG GAAAGTGGAA 
GTGAACGATC GTCAGGGTTT TGTGCCGGCT GCGTACGTGA AGAAATTGGA CCCCGCCCAG 
TCAGCCTCCC GGGAGAATCT CCTGGAGGAG CAAGGCAGCA TAGCACTGCG GCAGGAGCAG 
ATTGACAATC AGACACGCAT AACTAAGGAG GCCGGCAGTG TATCTCTGCG TATGAAGCAG 
GTGGAAGAAC TATATCATTC TCTGCTGGAA CTGGGTGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGTTGCA AGAAGTTTAT GTTGTTCCGT GAAGCGAATG AACTACAGCA ATGGATCAAT 
GAGAAGGAAG CCGCTCTGAC AAGTGAGGAG GTCGGAGCAG ACTTGGAGCA GGTTGAGGTG 
CTCCAGAAGA AGTTTGATGA CTTCCAGAAG GACCTGAAGG CCAATGAGTC ACGGTTGAAG 
GACATTAACA AGGTAGCTGA AGACCTGGAG TCTGAAGGTC TCATGGCAGA GGAGGTGCAG 
GCTGTGCAAC AACAGGAAGT GTATGGCATG ATGCCCAGGG ATGAAACTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGTCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTGA ATGAGCGCTG GCGGTCCCTA CAGCAGCTGG CCGAGGAACG GAGCCAGCTC 
TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CTGATGAAAC CAAAGAATGG 
ATTGAAGAGA AGAATCAAGC TCTAAACACA GACAATTATG GACATGATCT CGCCAGTGTC 
CAGGCCCTGC AACGCAAGCA TGAGGGCTTC GAGAGGGACC TTGCGGCTCT CGGTGACAAG 
GTAAACTCCC TTGGTGAAAC AGCAGAGCGC CTGATCCAGT CCCATCCCGA GTCAGCAGAA 
GACCTGCAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA GCAGCCTGGG GAAACGTGCA 
GATCAGCGCA AGGCAAAGTT GGGTGACTCC CACGACCTGC AGCGCTTCCT TAGCGATTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATA CGGGGGTTGG TGTCCTCAGA TGAGCTAGCC 
AAGGATGTCA CCGGAGCTGA GGCATTGCTG GAGCGACACC AGGAACACCG GACAGAAATC 
GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GACAGCAGCT GTTGGCTCAC 
GGACACTATG CCAGCCCTGA GATCAAGCAG AAACTTGATA TTCTTGACCA GGAGCGTGCA 
GACCTGGAGA AGGCCTGGGT TCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAACTGCAG 
CTGTTCCATC GGGACTGTGA GCAAGCTGAG AACTGGATGG CTGCCCGGGA GGCCTTCTTG 
AATACCGAAG ACAAAGGAGA CTCACTGGAC AGCGTAGAGG CTCTGATCAA AAAACATGAA 
GACTTTGACA AAGCGATTAA CGTCCAGGAA GAGAAGATTG CTGCTCTGCA GGCCTTTGCC 
GACCAGCTCA TCGCTGCCGG CCATTATGCC AAGGGAGACA TTTCTAGCCG GCGCAATGAG 
GTCTTGGACA GGTGGCGACG TCTGAAAGCC CAGATGATTG AGAAAAGGTC AAAGCTAGGA 
GAATCTCAAA CCCTCCAACA GTTCAGCCGG GATGTGGATG AGATTGAGGC TTGGATCAGT 
GAAAAATTGC AAACAGCGAG TGATGAGTCG TACAAGGATC CCACCAACAT CCAGCTTTCC 
AAGCTGCTGA GCAAGCACCA GAAGCACCAG GCTTTTGAAG CAGAGCTGCA TGCCAACGCT 
GACCGGATCC GTGGGGTTAT CGACATGGGC AACTCCCTCA TTGAACGTGG AGCCTGTGCC 
GGCAGTGAGG ATGCTGTCAA GGCCCGCCTG GCTGCCTTAG CTGACCAGTG GCAGTTCTTG 
GTGCAAAAGT CAGCGGAAAA GAGCCAGAAA CTGAAAGAAG CCAACAAGCA GCAGAACTTC 
AACACAGGGA TCAAGGACTT TGACTTCTGG CTGTCTGAGG TGGAGGCCCT GCTGGCATCC 
GAAGATTATG GCAAAGACCT AGCTTCTGTG AACAACCTGC TGAAAAAGCA TCAACTGCTG 
GAAGCAGATA TATCTGCCCA TGAGGATCGC CTGAAGGACC TGAACAGCCA GGCAGACAGC 
CTGATGACCA GCAGTGCCTT CGACACCTCC CAAGTAAAGG ACAAGAGGGA CACCATCAAC 
GGGCGCTTCC AGAAGATCAA GAGCATGGCG GCCTCCCGGC GAGCCAAGCT GAATGAATCC 
CATCGCCTGC ACCAGTTCTT CCGGGACATG GATGACGAGG AGTCCTGGAT CAAGGAGAAG 
AAGCTGCTGG TGGGCTCAGA GGACTACGGC CGGGACCTAA CCGGCGTGCA GAACCTGAGG 
AAGAAGCACA AGCGGCTGGA AGCAGAACTG GCTGCGCATG AGCCGGCTAT TCAGGGTGTC 
CTGGACACTG GCAAGAAGCT GTCCGATGAC AACACCATCG GGAAAGAGGA GATCCAGCAG 
CGGCTGGCGC AGTTTGTGGA GCACTGGAAA GAGCTGAAGC AGCTGGCAGC TGCCCGGGGT 
CAGCGGCTGG AAGAGTCCTT GGAATATCAG CAGTTTGTAG CCAATGTGGA AGAGGAAGAA 
GCCTGGATCA ATGAGAAAAT GACCCTGGTG GCCAGCGAAG ATTATGGCGA CACTCTTGCC 
GCCATCCAGG GCTTACTGAA GAAACATGAA GCTTTTGAGA CAGACTTCAC CGTCCACAAG 
GATCGCGTGA ATGATGTCTG CACCAATGGA CAAGACCTCA TTAAGAAGAA CAATCACCAT 
GAGGAGAACA TCTCTTCAAA GATGAAGGGC CTGAACGGGA AAGTGTCAGA CCTGGAGAAA 
GCTGCAGCCC AGAGAAAGGC GAAGCTGGAT GAGAACTCGG CCTTCCTTCA GTTCAACTGG 
AAGGCGGACG TGGTGGAGTC CTGGATCGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT 
TATGGCCGAG ACCTGTCTTC TGTGCAGACG CTCCTCACCA AACAGGAAAC TTTTGACGCT 
GGGCTGCAGG CCTTCCAGCA GGAAGGCATT GCCAACATCA CTGCCCTCAA AGATCAGCTT 
CTCGCCGCCA AACACGTTCA GTCCAAGGCC ATCGAGGCCC GGCACGCCTC CCTCATGAAG 
AGGTGGAGCC AGCTTCTGGC CAACTCAGCC GCCCGCAAGA AGAAGCTTCT GGAGGCTCAG 
AGTCACTTCC GCAAGGTGGA GGACCTCTTC CTGACCTTCG CCAAAAAGGC TTCTGCCTTC 
AACAGCTGGT TTGAAAATGC AGAGGAGGAC TTAACAGACC CCGTGCGCTG CAACTCCTTG 
GAAGAAATCA AAGCTTTGCG CGAGGCCCAC GACGCCTTCC GCTCCTCCCT CAGCTCTGCC 
CAGGCTGACT TCAACCAGCT GGCCGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTAGCC 
TCCAACCCCT ACACCTGGTT TACCATGGAG GCCCTGGAGG AGACCTGGAG GAACCTACAG 
AAAATCATCA AGGAGAGGGA GCTGGAGCTG CAGAAGGAAC AGCGGCGGCA GGAGGAGAAC 
GACAAGCTGC GCCAGGAGTT TGCCCAGCAC GCCAACGCCT TCCACCAGTG GATCCAAGAG 
ACCAGGTCCT GTATGGTGGA AGAGTCGGGG ACCCTCGAAT CCCAGCTTGA AGCTACCAAA 
CGCAAGCACC AGGAAATCCG AGCCATGAGA AGTCAGCTCA AAAAGATCGA GGACCTGGGG 
GCCGCCATGG AGGAGGCCCT CATCCTGGAC AACAAGTACA CGGAGCACAG CACCGTGGGC 
CTCGCCCAGC AGTGGGACCA GCTGGACCAG CTGGGCATGC GCATGCAGCA CAACCTGGAG 
CAGCAGATCC AGGCCAGGAA CACAACAGGT GTGACTGAGG AGGCCCTCAA AGAATTCAGC 
ATGATGTTTA AACACTTTGA CAAGGACAAG TCTGGCAGGC TGAACCATCA GGAGTTCAAA 
TCTTGCCTGC GCTCCCTGGG CTATGACCTG CCCATGGTGG AGGAAGGGGA ACCTGACCCT 
GAGTTCGAGG CAATCCTGGA CACGGTGGAT CCGAACAGAG ATGGCCATGT CTCCTTGCAA 
GAATACATGG CTTTCATGAT CAGCCGCGAA ACTGAGAACG TCAAGTCCAG CGAGGAGATT 
GAGAGCGCCT TCCGGGCCCT CAGCTCAGAG GGAAAGCCTT ACGTGACCAA GGAGGAGCTC 
TACCAGAACC TGACCCGGGA ACAAGCCGAC TACTGCGTCT CCCACATGAA GCCCTACGTG 
GACGGCAAGG GCCGCGAGCT CCCCACCGCG TTCGACTACG TGGAGTTCAC CCGCTCGCTT 
TTCGTGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006717313.1
CDS	14..7465
Translation	MLSSFRKRRVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELASDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMRRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPTDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRITKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGMMPRDETDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKQKLDILDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAAGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINGRFQKIKSMAASRRAKLNESHRLHQFFRDMDDEESWIKEKKLLVGSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHASLMKRWSQLLANSAARKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_006717250.1
Organism	Homo sapiens(human)
Definition	Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006717250.1

ORF Insert Sequence:

ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACCG ATACCACCGC 
TTCAAGGAAC TCTCAACCCT TAGGCGTCAG AAGCTGGAAG ATTCCTATCG ATTCCAGTTC 
TTTCAAAGAG ATGCTGAAGA GCTGGAGAAA TGGATACAGG AAAAACTTCA GATTGCATCT 
GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGAAAGC TTCAGAAGCA TCAAGCATTT 
GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAGC TGGATGAAAC TGGAAACCTG 
ATGATCTCAG AAGGGCATTT TGCATCTGAA ACCATACGGA CCCGTTTGAT GGAGCTGCAC 
CGCCAGTGGG AATTACTTTT GGAGAAGATG CGAGAAAAAG GAATCAAACT GCTGCAGGCC 
CAGAAGTTGG TGCAGTACTT ACGAGAATGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCAATTG TTACTTCTGA AGAGCTGGGC CAGGATCTGG AGCATGTAGA GGTTTTACAG 
AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCTCATG AAGAAAGAGT TAATGAAGTG 
AACCAGTTTG CTGCCAAACT CATACAGGAG CAGCACCCTG AGGAGGAACT GATCAAGACT 
AAGCAGGATG AAGTCAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCTCT GCAGAGGCAG 
GGGAAGCTCT TTGGGGCAGC AGAAGTTCAG CGCTTTAACA GGGATGTGGA TGAGACTATC 
AGTTGGATTA AGGAAAAGGA GCAGTTAATG GCCTCTGATG ATTTTGGCCG AGACCTGGCA 
AGTGTTCAGG CTCTGCTTCG GAAGCACGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAA 
GACAAGGTCA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCCCA CCCTCTGAGT 
GCAACACAGA TTCAAGTGAA GCGAGAGGAA CTGATTACAA ACTGGGAGCA GATCCGCACC 
TTGGCGGCAG AGAGACATGC ACGGCTCAAT GATTCATACA GGCTTCAACG CTTCCTTGCT 
GACTTCCGTG ACCTCACCAG CTGGGTGACT GAGATGAAAG CCCTCATCAA TGCAGATGAG 
CTTGCCAGTG ATGTGGCTGG GGCTGAAGCC CTGCTAGATA GACACCAAGA GCACAAGGGT 
GAAATTGATG CCCATGAAGA CAGCTTCAAA TCTGCAGATG AATCTGGACA GGCACTGCTT 
GCTGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCGTCCT TTCCGAGGAG 
AGAGCGGCGC TGCTGGAGCT GTGGGAGCTG CGCAGGCAGC AGTACGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGGGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ATGAAGACTT GGGAGATTCC TTGGATAGTG TGGAAGCGCT TCTTAAGAAG 
CACGAAGACT TTGAGAAATC CCTTAGTGCC CAGGAGGAAA AGATTACAGC ATTAGATGAA 
TTTGCAACCA AGCTAATTCA GAACAACCAC TATGCAATGG AAGATGTGGC CACTCGCCGA 
GATGCTCTGT TGAGCCGCCG CAATGCCCTT CACGAGAGAG CCATGCGTCG CCGGGCCCAG 
CTAGCCGATT CTTTCCATCT GCAGCAGTTT TTCCGTGATT CTGATGAGCT CAAGAGTTGG 
GTCAATGAGA AGATGAAAAC TGCCACAGAT GAAGCTTATA AAGATCCATC CAACCTACAA 
GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CAGCAAACCA GAGCCGAATT 
GATGCCTTGG AGAAAGCTGG CCAAAAGCTG ATTGATGTCA ACCACTATGC CAAGGATGAA 
GTGGCAGCTC GTATGAATGA GGTGATCAGT TTGTGGAAGA AACTGCTAGA GGCCACTGAA 
CTGAAAGGAA TAAAGCTTCG TGAAGCCAAC CAGCAACAGC AATTTAATCG CAATGTTGAG 
GATATTGAAT TGTGGCTATA TGAAGTAGAA GGTCACTTGG CTTCGGATGA TTACGGCAAA 
GATCTTACCA ATGTGCAGAA CCTCCAGAAG AAACATGCAC TGCTAGAGGC AGATGTGGCT 
GCTCACCAGG ACCGAATTGA TGGCATCACC ATTCAGGCCC GCCAGTTCCA AGATGCTGGC 
CATTTTGATG CAGAAAACAT CAAGAAGAAA CAGGAAGCCC TCGTGGCTCG CTATGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCTCTGCG GTTGCAGCAG 
CTCTTCCGGG ATGTTGAGGA TGAGGAGACG TGGATTCGAG AGAAAGAGCC CATTGCCGCA 
TCTACCAACA GAGGTAAGGA TTTAATTGGG GTCCAGAATC TGCTAAAGAA ACATCAAGCC 
TTACAAGCAG AAATTGCTGG ACATGAACCA CGCATCAAAG CAGTTACACA GAAGGGGAAT 
GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAGCT TCACGAGCTG 
AACCAAAAGT GGGAGGCACT GAAAGCCAAA GCTTCCCAGC GTCGGCAGGA CCTGGAGGAC 
TCTCTGCAGG CCCAGCAGTA CTTTGCTGAT GCTAACGAGG CTGAATCCTG GATGCGGGAG 
AAGGAACCCA TTGTGGGCAG CACTGACTAT GGCAAGGACG AAGACTCTGC TGAGGCTCTA 
CTGAAGAAAC ACGAAGCTTT GATGTCAGAT CTCAGTGCCT ACGGCAGCAG CATCCAGGCT 
TTGCGAGAAC AAGCACAGTC CTGCCGGCAA CAAGTGGCCC CCACGGATGA TGAGACTGGG 
AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAGGGAG ATATCCTTAC CTTACTCAAC AGCACCAACA AGGATTGGTG GAAAGTGGAA 
GTGAACGATC GTCAGGGTTT TGTGCCGGCT GCGTACGTGA AGAAATTGGA CCCCGCCCAG 
TCAGCCTCCC GGGAGAATCT CCTGGAGGAG CAAGGCAGCA TAGCACTGCG GCAGGAGCAG 
ATTGACAATC AGACACGCAT AACTAAGGAG GCCGGCAGTG TATCTCTGCG TATGAAGCAG 
GTGGAAGAAC TATATCATTC TCTGCTGGAA CTGGGTGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGTTGCA AGAAGTTTAT GTTGTTCCGT GAAGCGAATG AACTACAGCA ATGGATCAAT 
GAGAAGGAAG CCGCTCTGAC AAGTGAGGAG GTCGGAGCAG ACTTGGAGCA GGTTGAGGTG 
CTCCAGAAGA AGTTTGATGA CTTCCAGAAG GACCTGAAGG CCAATGAGTC ACGGTTGAAG 
GACATTAACA AGGTAGCTGA AGACCTGGAG TCTGAAGGTC TCATGGCAGA GGAGGTGCAG 
GCTGTGCAAC AACAGGAAGT GTATGGCATG ATGCCCAGGG ATGAAACTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGTCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTGA ATGAGCGCTG GCGGTCCCTA CAGCAGCTGG CCGAGGAACG GAGCCAGCTC 
TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CTGATGAAAC CAAAGAATGG 
ATTGAAGAGA AGAATCAAGC TCTAAACACA GACAATTATG GACATGATCT CGCCAGTGTC 
CAGGCCCTGC AACGCAAGCA TGAGGGCTTC GAGAGGGACC TTGCGGCTCT CGGTGACAAG 
GTAAACTCCC TTGGTGAAAC AGCAGAGCGC CTGATCCAGT CCCATCCCGA GTCAGCAGAA 
GACCTGCAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA GCAGCCTGGG GAAACGTGCA 
GATCAGCGCA AGGCAAAGTT GGGTGACTCC CACGACCTGC AGCGCTTCCT TAGCGATTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATA CGGGGGTTGG TGTCCTCAGA TGAGCTAGCC 
AAGGATGTCA CCGGAGCTGA GGCATTGCTG GAGCGACACC AGGAACACCG GACAGAAATC 
GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GACAGCAGCT GTTGGCTCAC 
GGACACTATG CCAGCCCTGA GATCAAGCAG AAACTTGATA TTCTTGACCA GGAGCGTGCA 
GACCTGGAGA AGGCCTGGGT TCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAACTGCAG 
CTGTTCCATC GGGACTGTGA GCAAGCTGAG AACTGGATGG CTGCCCGGGA GGCCTTCTTG 
AATACCGAAG ACAAAGGAGA CTCACTGGAC AGCGTAGAGG CTCTGATCAA AAAACATGAA 
GACTTTGACA AAGCGATTAA CGTCCAGGAA GAGAAGATTG CTGCTCTGCA GGCCTTTGCC 
GACCAGCTCA TCGCTGCCGG CCATTATGCC AAGGGAGACA TTTCTAGCCG GCGCAATGAG 
GTCTTGGACA GGTGGCGACG TCTGAAAGCC CAGATGATTG AGAAAAGGTC AAAGCTAGGA 
GAATCTCAAA CCCTCCAACA GTTCAGCCGG GATGTGGATG AGATTGAGGC TTGGATCAGT 
GAAAAATTGC AAACAGCGAG TGATGAGTCG TACAAGGATC CCACCAACAT CCAGCTTTCC 
AAGCTGCTGA GCAAGCACCA GAAGCACCAG GCTTTTGAAG CAGAGCTGCA TGCCAACGCT 
GACCGGATCC GTGGGGTTAT CGACATGGGC AACTCCCTCA TTGAACGTGG AGCCTGTGCC 
GGCAGTGAGG ATGCTGTCAA GGCCCGCCTG GCTGCCTTAG CTGACCAGTG GCAGTTCTTG 
GTGCAAAAGT CAGCGGAAAA GAGCCAGAAA CTGAAAGAAG CCAACAAGCA GCAGAACTTC 
AACACAGGGA TCAAGGACTT TGACTTCTGG CTGTCTGAGG TGGAGGCCCT GCTGGCATCC 
GAAGATTATG GCAAAGACCT AGCTTCTGTG AACAACCTGC TGAAAAAGCA TCAACTGCTG 
GAAGCAGATA TATCTGCCCA TGAGGATCGC CTGAAGGACC TGAACAGCCA GGCAGACAGC 
CTGATGACCA GCAGTGCCTT CGACACCTCC CAAGTAAAGG ACAAGAGGGA CACCATCAAC 
GGGCGCTTCC AGAAGATCAA GAGCATGGCG GCCTCCCGGC GAGCCAAGCT GAATGAATCC 
CATCGCCTGC ACCAGTTCTT CCGGGACATG GATGACGAGG AGTCCTGGAT CAAGGAGAAG 
AAGCTGCTGG TGGGCTCAGA GGACTACGGC CGGGACCTAA CCGGCGTGCA GAACCTGAGG 
AAGAAGCACA AGCGGCTGGA AGCAGAACTG GCTGCGCATG AGCCGGCTAT TCAGGGTGTC 
CTGGACACTG GCAAGAAGCT GTCCGATGAC AACACCATCG GGAAAGAGGA GATCCAGCAG 
CGGCTGGCGC AGTTTGTGGA GCACTGGAAA GAGCTGAAGC AGCTGGCAGC TGCCCGGGGT 
CAGCGGCTGG AAGAGTCCTT GGAATATCAG CAGTTTGTAG CCAATGTGGA AGAGGAAGAA 
GCCTGGATCA ATGAGAAAAT GACCCTGGTG GCCAGCGAAG ATTATGGCGA CACTCTTGCC 
GCCATCCAGG GCTTACTGAA GAAACATGAA GCTTTTGAGA CAGACTTCAC CGTCCACAAG 
GATCGCGTGA ATGATGTCTG CACCAATGGA CAAGACCTCA TTAAGAAGAA CAATCACCAT 
GAGGAGAACA TCTCTTCAAA GATGAAGGGC CTGAACGGGA AAGTGTCAGA CCTGGAGAAA 
GCTGCAGCCC AGAGAAAGGC GAAGCTGGAT GAGAACTCGG CCTTCCTTCA GTTCAACTGG 
AAGGCGGACG TGGTGGAGTC CTGGATCGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT 
TATGGCCGAG ACCTGTCTTC TGTGCAGACG CTCCTCACCA AACAGGAAAC TTTTGACGCT 
GGGCTGCAGG CCTTCCAGCA GGAAGGCATT GCCAACATCA CTGCCCTCAA AGATCAGCTT 
CTCGCCGCCA AACACGTTCA GTCCAAGGCC ATCGAGGCCC GGCACGCCTC CCTCATGAAG 
AGGTGGAGCC AGCTTCTGGC CAACTCAGCC GCCCGCAAGA AGAAGCTTCT GGAGGCTCAG 
AGTCACTTCC GCAAGGTGGA GGACCTCTTC CTGACCTTCG CCAAAAAGGC TTCTGCCTTC 
AACAGCTGGT TTGAAAATGC AGAGGAGGAC TTAACAGACC CCGTGCGCTG CAACTCCTTG 
GAAGAAATCA AAGCTTTGCG CGAGGCCCAC GACGCCTTCC GCTCCTCCCT CAGCTCTGCC 
CAGGCTGACT TCAACCAGCT GGCCGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTAGCC 
TCCAACCCCT ACACCTGGTT TACCATGGAG GCCCTGGAGG AGACCTGGAG GAACCTACAG 
AAAATCATCA AGGAGAGGGA GCTGGAGCTG CAGAAGGAAC AGCGGCGGCA GGAGGAGAAC 
GACAAGCTGC GCCAGGAGTT TGCCCAGCAC GCCAACGCCT TCCACCAGTG GATCCAAGAG 
ACCAGGTCCT GTATGGTGGA AGAGTCGGGG ACCCTCGAAT CCCAGCTTGA AGCTACCAAA 
CGCAAGCACC AGGAAATCCG AGCCATGAGA AGTCAGCTCA AAAAGATCGA GGACCTGGGG 
GCCGCCATGG AGGAGGCCCT CATCCTGGAC AACAAGTACA CGGAGCACAG CACCGTGGGC 
CTCGCCCAGC AGTGGGACCA GCTGGACCAG CTGGGCATGC GCATGCAGCA CAACCTGGAG 
CAGCAGATCC AGGCCAGGAA CACAACAGGT GTGACTGAGG AGGCCCTCAA AGAATTCAGC 
ATGATGTTTA AACACTTTGA CAAGGACAAG TCTGGCAGGC TGAACCATCA GGAGTTCAAA 
TCTTGCCTGC GCTCCCTGGG CTATGACCTG CCCATGGTGG AGGAAGGGGA ACCTGACCCT 
GAGTTCGAGG CAATCCTGGA CACGGTGGAT CCGAACAGAG ATGGCCATGT CTCCTTGCAA 
GAATACATGG CTTTCATGAT CAGCCGCGAA ACTGAGAACG TCAAGTCCAG CGAGGAGATT 
GAGAGCGCCT TCCGGGCCCT CAGCTCAGAG GGAAAGCCTT ACGTGACCAA GGAGGAGCTC 
TACCAGAACC TGACCCGGGA ACAAGCCGAC TACTGCGTCT CCCACATGAA GCCCTACGTG 
GACGGCAAGG GCCGCGAGCT CCCCACCGCG TTCGACTACG TGGAGTTCAC CCGCTCGCTT 
TTCGTGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_006717251.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006717314.1 Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X8, mRNA.

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CloneID	OHu43459
Clone ID Related Accession (Same CDS sequence)	XM_006717251.2
Accession Version	XM_006717251.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7437bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006717251.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAA GTGGGGTCAA AGTGCTGGAA ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTAG ACCGATACCA CCGCTTCAAG GAACTCTCAA CCCTTAGGCG TCAGAAGCTG 
GAAGATTCCT ATCGATTCCA GTTCTTTCAA AGAGATGCTG AAGAGCTGGA GAAATGGATA 
CAGGAAAAAC TTCAGATTGC ATCTGATGAG AATTATAAAG ACCCAACCAA CTTGCAGGGA 
AAGCTTCAGA AGCATCAAGC ATTTGAAGCT GAAGTGCAGG CCAACTCAGG AGCCATTGTT 
AAGCTGGATG AAACTGGAAA CCTGATGATC TCAGAAGGGC ATTTTGCATC TGAAACCATA 
CGGACCCGTT TGATGGAGCT GCACCGCCAG TGGGAATTAC TTTTGGAGAA GATGCGAGAA 
AAAGGAATCA AACTGCTGCA GGCCCAGAAG TTGGTGCAGT ACTTACGAGA ATGTGAGGAC 
GTGATGGACT GGATCAATGA CAAGGAAGCA ATTGTTACTT CTGAAGAGCT GGGCCAGGAT 
CTGGAGCATG TAGAGGTTTT ACAGAAGAAA TTTGAAGAGT TTCAAACAGA TATGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTGAACCAG TTTGCTGCCA AACTCATACA GGAGCAGCAC 
CCTGAGGAGG AACTGATCAA GACTAAGCAG GATGAAGTCA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CTCTGCAGAG GCAGGGGAAG CTCTTTGGGG CAGCAGAAGT TCAGCGCTTT 
AACAGGGATG TGGATGAGAC TATCAGTTGG ATTAAGGAAA AGGAGCAGTT AATGGCCTCT 
GATGATTTTG GCCGAGACCT GGCAAGTGTT CAGGCTCTGC TTCGGAAGCA CGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAAGACAAG GTCAAAGCCC TGTGTGCTGA GGCTGACCGC 
CTGCAACAGT CCCACCCTCT GAGTGCAACA CAGATTCAAG TGAAGCGAGA GGAACTGATT 
ACAAACTGGG AGCAGATCCG CACCTTGGCG GCAGAGAGAC ATGCACGGCT CAATGATTCA 
TACAGGCTTC AACGCTTCCT TGCTGACTTC CGTGACCTCA CCAGCTGGGT GACTGAGATG 
AAAGCCCTCA TCAATGCAGA TGAGCTTGCC AGTGATGTGG CTGGGGCTGA AGCCCTGCTA 
GATAGACACC AAGAGCACAA GGGTGAAATT GATGCCCATG AAGACAGCTT CAAATCTGCA 
GATGAATCTG GACAGGCACT GCTTGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGACCG TCCTTTCCGA GGAGAGAGCG GCGCTGCTGG AGCTGTGGGA GCTGCGCAGG 
CAGCAGTACG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GGGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAATGAAG ACTTGGGAGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTTAA GAAGCACGAA GACTTTGAGA AATCCCTTAG TGCCCAGGAG 
GAAAAGATTA CAGCATTAGA TGAATTTGCA ACCAAGCTAA TTCAGAACAA CCACTATGCA 
ATGGAAGATG TGGCCACTCG CCGAGATGCT CTGTTGAGCC GCCGCAATGC CCTTCACGAG 
AGAGCCATGC GTCGCCGGGC CCAGCTAGCC GATTCTTTCC ATCTGCAGCA GTTTTTCCGT 
GATTCTGATG AGCTCAAGAG TTGGGTCAAT GAGAAGATGA AAACTGCCAC AGATGAAGCT 
TATAAAGATC CATCCAACCT ACAAGGAAAA GTACAGAAGC ATCAGGCTTT TGAGGCTGAG 
CTCTCAGCAA ACCAGAGCCG AATTGATGCC TTGGAGAAAG CTGGCCAAAA GCTGATTGAT 
GTCAACCACT ATGCCAAGGA TGAAGTGGCA GCTCGTATGA ATGAGGTGAT CAGTTTGTGG 
AAGAAACTGC TAGAGGCCAC TGAACTGAAA GGAATAAAGC TTCGTGAAGC CAACCAGCAA 
CAGCAATTTA ATCGCAATGT TGAGGATATT GAATTGTGGC TATATGAAGT AGAAGGTCAC 
TTGGCTTCGG ATGATTACGG CAAAGATCTT ACCAATGTGC AGAACCTCCA GAAGAAACAT 
GCACTGCTAG AGGCAGATGT GGCTGCTCAC CAGGACCGAA TTGATGGCAT CACCATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTT GATGCAGAAA ACATCAAGAA GAAACAGGAA 
GCCCTCGTGG CTCGCTATGA GGCACTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCCGATTCTC TGCGGTTGCA GCAGCTCTTC CGGGATGTTG AGGATGAGGA GACGTGGATT 
CGAGAGAAAG AGCCCATTGC CGCATCTACC AACAGAGGTA AGGATTTAAT TGGGGTCCAG 
AATCTGCTAA AGAAACATCA AGCCTTACAA GCAGAAATTG CTGGACATGA ACCACGCATC 
AAAGCAGTTA CACAGAAGGG GAATGCCATG GTGGAGGAAG GCCATTTTGC TGCAGAGGAT 
GTGAAGGCCA AGCTTCACGA GCTGAACCAA AAGTGGGAGG CACTGAAAGC CAAAGCTTCC 
CAGCGTCGGC AGGACCTGGA GGACTCTCTG CAGGCCCAGC AGTACTTTGC TGATGCTAAC 
GAGGCTGAAT CCTGGATGCG GGAGAAGGAA CCCATTGTGG GCAGCACTGA CTATGGCAAG 
GACGAAGACT CTGCTGAGGC TCTACTGAAG AAACACGAAG CTTTGATGTC AGATCTCAGT 
GCCTACGGCA GCAGCATCCA GGCTTTGCGA GAACAAGCAC AGTCCTGCCG GCAACAAGTG 
GCCCCCACGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CTCTCTACGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAG GGAGATATCC TTACCTTACT CAACAGCACC 
AACAAGGATT GGTGGAAAGT GGAAGTGAAC GATCGTCAGG GTTTTGTGCC GGCTGCGTAC 
GTGAAGAAAT TGGACCCCGC CCAGTCAGCC TCCCGGGAGA ATCTCCTGGA GGAGCAAGGC 
AGCATAGCAC TGCGGCAGGA GCAGATTGAC AATCAATATC ATTCTCTGCT GGAACTGGGT 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TTATGTTGTT CCGTGAAGCG 
AATGAACTAC AGCAATGGAT CAATGAGAAG GAAGCCGCTC TGACAAGTGA GGAGGTCGGA 
GCAGACTTGG AGCAGGTTGA GGTGCTCCAG AAGAAGTTTG ATGACTTCCA GAAGGACCTG 
AAGGCCAATG AGTCACGGTT GAAGGACATT AACAAGGTAG CTGAAGACCT GGAGTCTGAA 
GGTCTCATGG CAGAGGAGGT GCAGGCTGTG CAACAACAGG AAGTGTATGG CATGATGCCC 
AGGGATGAAA CTGATTCCAA GACAGCCTCC CCGTGGAAGT CTGCTCGTCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTACAGCAG 
CTGGCCGAGG AACGGAGCCA GCTCTTGGGC AGCGCCCATG AAGTACAGAG GTTCCACAGA 
GATGCTGATG AAACCAAAGA ATGGATTGAA GAGAAGAATC AAGCTCTAAA CACAGACAAT 
TATGGACATG ATCTCGCCAG TGTCCAGGCC CTGCAACGCA AGCATGAGGG CTTCGAGAGG 
GACCTTGCGG CTCTCGGTGA CAAGGTAAAC TCCCTTGGTG AAACAGCAGA GCGCCTGATC 
CAGTCCCATC CCGAGTCAGC AGAAGACCTG CAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGAGCAGCC TGGGGAAACG TGCAGATCAG CGCAAGGCAA AGTTGGGTGA CTCCCACGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGGGGG 
TTGGTGTCCT CAGATGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCATT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATCGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGACAGC AGCTGTTGGC TCACGGACAC TATGCCAGCC CTGAGATCAA GCAGAAACTT 
GATATTCTTG ACCAGGAGCG TGCAGACCTG GAGAAGGCCT GGGTTCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAACT GCAGCTGTTC CATCGGGACT GTGAGCAAGC TGAGAACTGG 
ATGGCTGCCC GGGAGGCCTT CTTGAATACC GAAGACAAAG GAGACTCACT GGACAGCGTA 
GAGGCTCTGA TCAAAAAACA TGAAGACTTT GACAAAGCGA TTAACGTCCA GGAAGAGAAG 
ATTGCTGCTC TGCAGGCCTT TGCCGACCAG CTCATCGCTG CCGGCCATTA TGCCAAGGGA 
GACATTTCTA GCCGGCGCAA TGAGGTCTTG GACAGGTGGC GACGTCTGAA AGCCCAGATG 
ATTGAGAAAA GGTCAAAGCT AGGAGAATCT CAAACCCTCC AACAGTTCAG CCGGGATGTG 
GATGAGATTG AGGCTTGGAT CAGTGAAAAA TTGCAAACAG CGAGTGATGA GTCGTACAAG 
GATCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ACCAGAAGCA CCAGGCTTTT 
GAAGCAGAGC TGCATGCCAA CGCTGACCGG ATCCGTGGGG TTATCGACAT GGGCAACTCC 
CTCATTGAAC GTGGAGCCTG TGCCGGCAGT GAGGATGCTG TCAAGGCCCG CCTGGCTGCC 
TTAGCTGACC AGTGGCAGTT CTTGGTGCAA AAGTCAGCGG AAAAGAGCCA GAAACTGAAA 
GAAGCCAACA AGCAGCAGAA CTTCAACACA GGGATCAAGG ACTTTGACTT CTGGCTGTCT 
GAGGTGGAGG CCCTGCTGGC ATCCGAAGAT TATGGCAAAG ACCTAGCTTC TGTGAACAAC 
CTGCTGAAAA AGCATCAACT GCTGGAAGCA GATATATCTG CCCATGAGGA TCGCCTGAAG 
GACCTGAACA GCCAGGCAGA CAGCCTGATG ACCAGCAGTG CCTTCGACAC CTCCCAAGTA 
AAGGACAAGA GGGACACCAT CAACGGGCGC TTCCAGAAGA TCAAGAGCAT GGCGGCCTCC 
CGGCGAGCCA AGCTGAATGA ATCCCATCGC CTGCACCAGT TCTTCCGGGA CATGGATGAC 
GAGGAGTCCT GGATCAAGGA GAAGAAGCTG CTGGTGGGCT CAGAGGACTA CGGCCGGGAC 
CTAACCGGCG TGCAGAACCT GAGGAAGAAG CACAAGCGGC TGGAAGCAGA ACTGGCTGCG 
CATGAGCCGG CTATTCAGGG TGTCCTGGAC ACTGGCAAGA AGCTGTCCGA TGACAACACC 
ATCGGGAAAG AGGAGATCCA GCAGCGGCTG GCGCAGTTTG TGGAGCACTG GAAAGAGCTG 
AAGCAGCTGG CAGCTGCCCG GGGTCAGCGG CTGGAAGAGT CCTTGGAATA TCAGCAGTTT 
GTAGCCAATG TGGAAGAGGA AGAAGCCTGG ATCAATGAGA AAATGACCCT GGTGGCCAGC 
GAAGATTATG GCGACACTCT TGCCGCCATC CAGGGCTTAC TGAAGAAACA TGAAGCTTTT 
GAGACAGACT TCACCGTCCA CAAGGATCGC GTGAATGATG TCTGCACCAA TGGACAAGAC 
CTCATTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CAAAGATGAA GGGCCTGAAC 
GGGAAAGTGT CAGACCTGGA GAAAGCTGCA GCCCAGAGAA AGGCGAAGCT GGATGAGAAC 
TCGGCCTTCC TTCAGTTCAA CTGGAAGGCG GACGTGGTGG AGTCCTGGAT CGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGC CGAGACCTGT CTTCTGTGCA GACGCTCCTC 
ACCAAACAGG AAACTTTTGA CGCTGGGCTG CAGGCCTTCC AGCAGGAAGG CATTGCCAAC 
ATCACTGCCC TCAAAGATCA GCTTCTCGCC GCCAAACACG TTCAGTCCAA GGCCATCGAG 
GCCCGGCACG CCTCCCTCAT GAAGAGGTGG AGCCAGCTTC TGGCCAACTC AGCCGCCCGC 
AAGAAGAAGC TTCTGGAGGC TCAGAGTCAC TTCCGCAAGG TGGAGGACCT CTTCCTGACC 
TTCGCCAAAA AGGCTTCTGC CTTCAACAGC TGGTTTGAAA ATGCAGAGGA GGACTTAACA 
GACCCCGTGC GCTGCAACTC CTTGGAAGAA ATCAAAGCTT TGCGCGAGGC CCACGACGCC 
TTCCGCTCCT CCCTCAGCTC TGCCCAGGCT GACTTCAACC AGCTGGCCGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT AGCCTCCAAC CCCTACACCT GGTTTACCAT GGAGGCCCTG 
GAGGAGACCT GGAGGAACCT ACAGAAAATC ATCAAGGAGA GGGAGCTGGA GCTGCAGAAG 
GAACAGCGGC GGCAGGAGGA GAACGACAAG CTGCGCCAGG AGTTTGCCCA GCACGCCAAC 
GCCTTCCACC AGTGGATCCA AGAGACCAGG ACATACCTCC TCGATGGCAT AGCATATCGT 
CGGGTCATTC GTGTCTATCA GTATGAAGTT GGGGATGATC TGTCTGGAAG GTCCTGTATG 
GTGGAAGAGT CGGGGACCCT CGAATCCCAG CTTGAAGCTA CCAAACGCAA GCACCAGGAA 
ATCCGAGCCA TGAGAAGTCA GCTCAAAAAG ATCGAGGACC TGGGGGCCGC CATGGAGGAG 
GCCCTCATCC TGGACAACAA GTACACGGAG CACAGCACCG TGGGCCTCGC CCAGCAGTGG 
GACCAGCTGG ACCAGCTGGG CATGCGCATG CAGCACAACC TGGAGCAGCA GATCCAGGCC 
AGGAACACAA CAGGTGTGAC TGAGGAGGCC CTCAAAGAAT TCAGCATGAT GTTTAAACAC 
TTTGACAAGG ACAAGTCTGG CAGGCTGAAC CATCAGGAGT TCAAATCTTG CCTGCGCTCC 
CTGGGCTATG ACCTGCCCAT GGTGGAGGAA GGGGAACCTG ACCCTGAGTT CGAGGCAATC 
CTGGACACGG TGGATCCGAA CAGAGATGGC CATGTCTCCT TGCAAGAATA CATGGCTTTC 
ATGATCAGCC GCGAAACTGA GAACGTCAAG TCCAGCGAGG AGATTGAGAG CGCCTTCCGG 
GCCCTCAGCT CAGAGGGAAA GCCTTACGTG ACCAAGGAGG AGCTCTACCA GAACCTGACC 
CGGGAACAAG CCGACTACTG CGTCTCCCAC ATGAAGCCCT ACGTGGACGG CAAGGGCCGC 
GAGCTCCCCA CCGCGTTCGA CTACGTGGAG TTCACCCGCT CGCTTTTCGT GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006717314.1
CDS	137..7573
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELASDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMRRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPTDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGMMPRDETDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKQKLDILDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAAGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINGRFQKIKSMAASRRAKLNESHRLHQFFRDMDDEESWIKEKKLLVGSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHASLMKRWSQLLANSAARKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGIAYRRVIRVYQYEVGDDLSGRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_006717251.2
Organism	Homo sapiens(human)
Definition	Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006717251.2

ORF Insert Sequence:

ATGGACCCAA GTGGGGTCAA AGTGCTGGAA ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTAG ACCGATACCA CCGCTTCAAG GAACTCTCAA CCCTTAGGCG TCAGAAGCTG 
GAAGATTCCT ATCGATTCCA GTTCTTTCAA AGAGATGCTG AAGAGCTGGA GAAATGGATA 
CAGGAAAAAC TTCAGATTGC ATCTGATGAG AATTATAAAG ACCCAACCAA CTTGCAGGGA 
AAGCTTCAGA AGCATCAAGC ATTTGAAGCT GAAGTGCAGG CCAACTCAGG AGCCATTGTT 
AAGCTGGATG AAACTGGAAA CCTGATGATC TCAGAAGGGC ATTTTGCATC TGAAACCATA 
CGGACCCGTT TGATGGAGCT GCACCGCCAG TGGGAATTAC TTTTGGAGAA GATGCGAGAA 
AAAGGAATCA AACTGCTGCA GGCCCAGAAG TTGGTGCAGT ACTTACGAGA ATGTGAGGAC 
GTGATGGACT GGATCAATGA CAAGGAAGCA ATTGTTACTT CTGAAGAGCT GGGCCAGGAT 
CTGGAGCATG TAGAGGTTTT ACAGAAGAAA TTTGAAGAGT TTCAAACAGA TATGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTGAACCAG TTTGCTGCCA AACTCATACA GGAGCAGCAC 
CCTGAGGAGG AACTGATCAA GACTAAGCAG GATGAAGTCA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CTCTGCAGAG GCAGGGGAAG CTCTTTGGGG CAGCAGAAGT TCAGCGCTTT 
AACAGGGATG TGGATGAGAC TATCAGTTGG ATTAAGGAAA AGGAGCAGTT AATGGCCTCT 
GATGATTTTG GCCGAGACCT GGCAAGTGTT CAGGCTCTGC TTCGGAAGCA CGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAAGACAAG GTCAAAGCCC TGTGTGCTGA GGCTGACCGC 
CTGCAACAGT CCCACCCTCT GAGTGCAACA CAGATTCAAG TGAAGCGAGA GGAACTGATT 
ACAAACTGGG AGCAGATCCG CACCTTGGCG GCAGAGAGAC ATGCACGGCT CAATGATTCA 
TACAGGCTTC AACGCTTCCT TGCTGACTTC CGTGACCTCA CCAGCTGGGT GACTGAGATG 
AAAGCCCTCA TCAATGCAGA TGAGCTTGCC AGTGATGTGG CTGGGGCTGA AGCCCTGCTA 
GATAGACACC AAGAGCACAA GGGTGAAATT GATGCCCATG AAGACAGCTT CAAATCTGCA 
GATGAATCTG GACAGGCACT GCTTGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGACCG TCCTTTCCGA GGAGAGAGCG GCGCTGCTGG AGCTGTGGGA GCTGCGCAGG 
CAGCAGTACG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GGGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAATGAAG ACTTGGGAGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTTAA GAAGCACGAA GACTTTGAGA AATCCCTTAG TGCCCAGGAG 
GAAAAGATTA CAGCATTAGA TGAATTTGCA ACCAAGCTAA TTCAGAACAA CCACTATGCA 
ATGGAAGATG TGGCCACTCG CCGAGATGCT CTGTTGAGCC GCCGCAATGC CCTTCACGAG 
AGAGCCATGC GTCGCCGGGC CCAGCTAGCC GATTCTTTCC ATCTGCAGCA GTTTTTCCGT 
GATTCTGATG AGCTCAAGAG TTGGGTCAAT GAGAAGATGA AAACTGCCAC AGATGAAGCT 
TATAAAGATC CATCCAACCT ACAAGGAAAA GTACAGAAGC ATCAGGCTTT TGAGGCTGAG 
CTCTCAGCAA ACCAGAGCCG AATTGATGCC TTGGAGAAAG CTGGCCAAAA GCTGATTGAT 
GTCAACCACT ATGCCAAGGA TGAAGTGGCA GCTCGTATGA ATGAGGTGAT CAGTTTGTGG 
AAGAAACTGC TAGAGGCCAC TGAACTGAAA GGAATAAAGC TTCGTGAAGC CAACCAGCAA 
CAGCAATTTA ATCGCAATGT TGAGGATATT GAATTGTGGC TATATGAAGT AGAAGGTCAC 
TTGGCTTCGG ATGATTACGG CAAAGATCTT ACCAATGTGC AGAACCTCCA GAAGAAACAT 
GCACTGCTAG AGGCAGATGT GGCTGCTCAC CAGGACCGAA TTGATGGCAT CACCATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTT GATGCAGAAA ACATCAAGAA GAAACAGGAA 
GCCCTCGTGG CTCGCTATGA GGCACTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCCGATTCTC TGCGGTTGCA GCAGCTCTTC CGGGATGTTG AGGATGAGGA GACGTGGATT 
CGAGAGAAAG AGCCCATTGC CGCATCTACC AACAGAGGTA AGGATTTAAT TGGGGTCCAG 
AATCTGCTAA AGAAACATCA AGCCTTACAA GCAGAAATTG CTGGACATGA ACCACGCATC 
AAAGCAGTTA CACAGAAGGG GAATGCCATG GTGGAGGAAG GCCATTTTGC TGCAGAGGAT 
GTGAAGGCCA AGCTTCACGA GCTGAACCAA AAGTGGGAGG CACTGAAAGC CAAAGCTTCC 
CAGCGTCGGC AGGACCTGGA GGACTCTCTG CAGGCCCAGC AGTACTTTGC TGATGCTAAC 
GAGGCTGAAT CCTGGATGCG GGAGAAGGAA CCCATTGTGG GCAGCACTGA CTATGGCAAG 
GACGAAGACT CTGCTGAGGC TCTACTGAAG AAACACGAAG CTTTGATGTC AGATCTCAGT 
GCCTACGGCA GCAGCATCCA GGCTTTGCGA GAACAAGCAC AGTCCTGCCG GCAACAAGTG 
GCCCCCACGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CTCTCTACGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAG GGAGATATCC TTACCTTACT CAACAGCACC 
AACAAGGATT GGTGGAAAGT GGAAGTGAAC GATCGTCAGG GTTTTGTGCC GGCTGCGTAC 
GTGAAGAAAT TGGACCCCGC CCAGTCAGCC TCCCGGGAGA ATCTCCTGGA GGAGCAAGGC 
AGCATAGCAC TGCGGCAGGA GCAGATTGAC AATCAATATC ATTCTCTGCT GGAACTGGGT 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TTATGTTGTT CCGTGAAGCG 
AATGAACTAC AGCAATGGAT CAATGAGAAG GAAGCCGCTC TGACAAGTGA GGAGGTCGGA 
GCAGACTTGG AGCAGGTTGA GGTGCTCCAG AAGAAGTTTG ATGACTTCCA GAAGGACCTG 
AAGGCCAATG AGTCACGGTT GAAGGACATT AACAAGGTAG CTGAAGACCT GGAGTCTGAA 
GGTCTCATGG CAGAGGAGGT GCAGGCTGTG CAACAACAGG AAGTGTATGG CATGATGCCC 
AGGGATGAAA CTGATTCCAA GACAGCCTCC CCGTGGAAGT CTGCTCGTCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTACAGCAG 
CTGGCCGAGG AACGGAGCCA GCTCTTGGGC AGCGCCCATG AAGTACAGAG GTTCCACAGA 
GATGCTGATG AAACCAAAGA ATGGATTGAA GAGAAGAATC AAGCTCTAAA CACAGACAAT 
TATGGACATG ATCTCGCCAG TGTCCAGGCC CTGCAACGCA AGCATGAGGG CTTCGAGAGG 
GACCTTGCGG CTCTCGGTGA CAAGGTAAAC TCCCTTGGTG AAACAGCAGA GCGCCTGATC 
CAGTCCCATC CCGAGTCAGC AGAAGACCTG CAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGAGCAGCC TGGGGAAACG TGCAGATCAG CGCAAGGCAA AGTTGGGTGA CTCCCACGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGGGGG 
TTGGTGTCCT CAGATGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCATT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATCGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGACAGC AGCTGTTGGC TCACGGACAC TATGCCAGCC CTGAGATCAA GCAGAAACTT 
GATATTCTTG ACCAGGAGCG TGCAGACCTG GAGAAGGCCT GGGTTCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAACT GCAGCTGTTC CATCGGGACT GTGAGCAAGC TGAGAACTGG 
ATGGCTGCCC GGGAGGCCTT CTTGAATACC GAAGACAAAG GAGACTCACT GGACAGCGTA 
GAGGCTCTGA TCAAAAAACA TGAAGACTTT GACAAAGCGA TTAACGTCCA GGAAGAGAAG 
ATTGCTGCTC TGCAGGCCTT TGCCGACCAG CTCATCGCTG CCGGCCATTA TGCCAAGGGA 
GACATTTCTA GCCGGCGCAA TGAGGTCTTG GACAGGTGGC GACGTCTGAA AGCCCAGATG 
ATTGAGAAAA GGTCAAAGCT AGGAGAATCT CAAACCCTCC AACAGTTCAG CCGGGATGTG 
GATGAGATTG AGGCTTGGAT CAGTGAAAAA TTGCAAACAG CGAGTGATGA GTCGTACAAG 
GATCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ACCAGAAGCA CCAGGCTTTT 
GAAGCAGAGC TGCATGCCAA CGCTGACCGG ATCCGTGGGG TTATCGACAT GGGCAACTCC 
CTCATTGAAC GTGGAGCCTG TGCCGGCAGT GAGGATGCTG TCAAGGCCCG CCTGGCTGCC 
TTAGCTGACC AGTGGCAGTT CTTGGTGCAA AAGTCAGCGG AAAAGAGCCA GAAACTGAAA 
GAAGCCAACA AGCAGCAGAA CTTCAACACA GGGATCAAGG ACTTTGACTT CTGGCTGTCT 
GAGGTGGAGG CCCTGCTGGC ATCCGAAGAT TATGGCAAAG ACCTAGCTTC TGTGAACAAC 
CTGCTGAAAA AGCATCAACT GCTGGAAGCA GATATATCTG CCCATGAGGA TCGCCTGAAG 
GACCTGAACA GCCAGGCAGA CAGCCTGATG ACCAGCAGTG CCTTCGACAC CTCCCAAGTA 
AAGGACAAGA GGGACACCAT CAACGGGCGC TTCCAGAAGA TCAAGAGCAT GGCGGCCTCC 
CGGCGAGCCA AGCTGAATGA ATCCCATCGC CTGCACCAGT TCTTCCGGGA CATGGATGAC 
GAGGAGTCCT GGATCAAGGA GAAGAAGCTG CTGGTGGGCT CAGAGGACTA CGGCCGGGAC 
CTAACCGGCG TGCAGAACCT GAGGAAGAAG CACAAGCGGC TGGAAGCAGA ACTGGCTGCG 
CATGAGCCGG CTATTCAGGG TGTCCTGGAC ACTGGCAAGA AGCTGTCCGA TGACAACACC 
ATCGGGAAAG AGGAGATCCA GCAGCGGCTG GCGCAGTTTG TGGAGCACTG GAAAGAGCTG 
AAGCAGCTGG CAGCTGCCCG GGGTCAGCGG CTGGAAGAGT CCTTGGAATA TCAGCAGTTT 
GTAGCCAATG TGGAAGAGGA AGAAGCCTGG ATCAATGAGA AAATGACCCT GGTGGCCAGC 
GAAGATTATG GCGACACTCT TGCCGCCATC CAGGGCTTAC TGAAGAAACA TGAAGCTTTT 
GAGACAGACT TCACCGTCCA CAAGGATCGC GTGAATGATG TCTGCACCAA TGGACAAGAC 
CTCATTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CAAAGATGAA GGGCCTGAAC 
GGGAAAGTGT CAGACCTGGA GAAAGCTGCA GCCCAGAGAA AGGCGAAGCT GGATGAGAAC 
TCGGCCTTCC TTCAGTTCAA CTGGAAGGCG GACGTGGTGG AGTCCTGGAT CGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGC CGAGACCTGT CTTCTGTGCA GACGCTCCTC 
ACCAAACAGG AAACTTTTGA CGCTGGGCTG CAGGCCTTCC AGCAGGAAGG CATTGCCAAC 
ATCACTGCCC TCAAAGATCA GCTTCTCGCC GCCAAACACG TTCAGTCCAA GGCCATCGAG 
GCCCGGCACG CCTCCCTCAT GAAGAGGTGG AGCCAGCTTC TGGCCAACTC AGCCGCCCGC 
AAGAAGAAGC TTCTGGAGGC TCAGAGTCAC TTCCGCAAGG TGGAGGACCT CTTCCTGACC 
TTCGCCAAAA AGGCTTCTGC CTTCAACAGC TGGTTTGAAA ATGCAGAGGA GGACTTAACA 
GACCCCGTGC GCTGCAACTC CTTGGAAGAA ATCAAAGCTT TGCGCGAGGC CCACGACGCC 
TTCCGCTCCT CCCTCAGCTC TGCCCAGGCT GACTTCAACC AGCTGGCCGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT AGCCTCCAAC CCCTACACCT GGTTTACCAT GGAGGCCCTG 
GAGGAGACCT GGAGGAACCT ACAGAAAATC ATCAAGGAGA GGGAGCTGGA GCTGCAGAAG 
GAACAGCGGC GGCAGGAGGA GAACGACAAG CTGCGCCAGG AGTTTGCCCA GCACGCCAAC 
GCCTTCCACC AGTGGATCCA AGAGACCAGG ACATACCTCC TCGATGGCAT AGCATATCGT 
CGGGTCATTC GTGTCTATCA GTATGAAGTT GGGGATGATC TGTCTGGAAG GTCCTGTATG 
GTGGAAGAGT CGGGGACCCT CGAATCCCAG CTTGAAGCTA CCAAACGCAA GCACCAGGAA 
ATCCGAGCCA TGAGAAGTCA GCTCAAAAAG ATCGAGGACC TGGGGGCCGC CATGGAGGAG 
GCCCTCATCC TGGACAACAA GTACACGGAG CACAGCACCG TGGGCCTCGC CCAGCAGTGG 
GACCAGCTGG ACCAGCTGGG CATGCGCATG CAGCACAACC TGGAGCAGCA GATCCAGGCC 
AGGAACACAA CAGGTGTGAC TGAGGAGGCC CTCAAAGAAT TCAGCATGAT GTTTAAACAC 
TTTGACAAGG ACAAGTCTGG CAGGCTGAAC CATCAGGAGT TCAAATCTTG CCTGCGCTCC 
CTGGGCTATG ACCTGCCCAT GGTGGAGGAA GGGGAACCTG ACCCTGAGTT CGAGGCAATC 
CTGGACACGG TGGATCCGAA CAGAGATGGC CATGTCTCCT TGCAAGAATA CATGGCTTTC 
ATGATCAGCC GCGAAACTGA GAACGTCAAG TCCAGCGAGG AGATTGAGAG CGCCTTCCGG 
GCCCTCAGCT CAGAGGGAAA GCCTTACGTG ACCAAGGAGG AGCTCTACCA GAACCTGACC 
CGGGAACAAG CCGACTACTG CGTCTCCCAC ATGAAGCCCT ACGTGGACGG CAAGGGCCGC 
GAGCTCCCCA CCGCGTTCGA CTACGTGGAG TTCACCCGCT CGCTTTTCGT GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_006717252.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006717315.1 Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X10, mRNA.

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CloneID	OHu43460
Clone ID Related Accession (Same CDS sequence)	XM_006717252.2 , XM_006717252.3
Accession Version	XM_006717252.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7410bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006717252.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACCG ATACCACCGC 
TTCAAGGAAC TCTCAACCCT TAGGCGTCAG AAGCTGGAAG ATTCCTATCG ATTCCAGTTC 
TTTCAAAGAG ATGCTGAAGA GCTGGAGAAA TGGATACAGG AAAAACTTCA GATTGCATCT 
GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGAAAGC TTCAGAAGCA TCAAGCATTT 
GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAGC TGGATGAAAC TGGAAACCTG 
ATGATCTCAG AAGGGCATTT TGCATCTGAA ACCATACGGA CCCGTTTGAT GGAGCTGCAC 
CGCCAGTGGG AATTACTTTT GGAGAAGATG CGAGAAAAAG GAATCAAACT GCTGCAGGCC 
CAGAAGTTGG TGCAGTACTT ACGAGAATGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCAATTG TTACTTCTGA AGAGCTGGGC CAGGATCTGG AGCATGTAGA GGTTTTACAG 
AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCTCATG AAGAAAGAGT TAATGAAGTG 
AACCAGTTTG CTGCCAAACT CATACAGGAG CAGCACCCTG AGGAGGAACT GATCAAGACT 
AAGCAGGATG AAGTCAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCTCT GCAGAGGCAG 
GGGAAGCTCT TTGGGGCAGC AGAAGTTCAG CGCTTTAACA GGGATGTGGA TGAGACTATC 
AGTTGGATTA AGGAAAAGGA GCAGTTAATG GCCTCTGATG ATTTTGGCCG AGACCTGGCA 
AGTGTTCAGG CTCTGCTTCG GAAGCACGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAA 
GACAAGGTCA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCCCA CCCTCTGAGT 
GCAACACAGA TTCAAGTGAA GCGAGAGGAA CTGATTACAA ACTGGGAGCA GATCCGCACC 
TTGGCGGCAG AGAGACATGC ACGGCTCAAT GATTCATACA GGCTTCAACG CTTCCTTGCT 
GACTTCCGTG ACCTCACCAG CTGGGTGACT GAGATGAAAG CCCTCATCAA TGCAGATGAG 
CTTGCCAGTG ATGTGGCTGG GGCTGAAGCC CTGCTAGATA GACACCAAGA GCACAAGGGT 
GAAATTGATG CCCATGAAGA CAGCTTCAAA TCTGCAGATG AATCTGGACA GGCACTGCTT 
GCTGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCGTCCT TTCCGAGGAG 
AGAGCGGCGC TGCTGGAGCT GTGGGAGCTG CGCAGGCAGC AGTACGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGGGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ATGAAGACTT GGGAGATTCC TTGGATAGTG TGGAAGCGCT TCTTAAGAAG 
CACGAAGACT TTGAGAAATC CCTTAGTGCC CAGGAGGAAA AGATTACAGC ATTAGATGAA 
TTTGCAACCA AGCTAATTCA GAACAACCAC TATGCAATGG AAGATGTGGC CACTCGCCGA 
GATGCTCTGT TGAGCCGCCG CAATGCCCTT CACGAGAGAG CCATGCGTCG CCGGGCCCAG 
CTAGCCGATT CTTTCCATCT GCAGCAGTTT TTCCGTGATT CTGATGAGCT CAAGAGTTGG 
GTCAATGAGA AGATGAAAAC TGCCACAGAT GAAGCTTATA AAGATCCATC CAACCTACAA 
GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CAGCAAACCA GAGCCGAATT 
GATGCCTTGG AGAAAGCTGG CCAAAAGCTG ATTGATGTCA ACCACTATGC CAAGGATGAA 
GTGGCAGCTC GTATGAATGA GGTGATCAGT TTGTGGAAGA AACTGCTAGA GGCCACTGAA 
CTGAAAGGAA TAAAGCTTCG TGAAGCCAAC CAGCAACAGC AATTTAATCG CAATGTTGAG 
GATATTGAAT TGTGGCTATA TGAAGTAGAA GGTCACTTGG CTTCGGATGA TTACGGCAAA 
GATCTTACCA ATGTGCAGAA CCTCCAGAAG AAACATGCAC TGCTAGAGGC AGATGTGGCT 
GCTCACCAGG ACCGAATTGA TGGCATCACC ATTCAGGCCC GCCAGTTCCA AGATGCTGGC 
CATTTTGATG CAGAAAACAT CAAGAAGAAA CAGGAAGCCC TCGTGGCTCG CTATGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCTCTGCG GTTGCAGCAG 
CTCTTCCGGG ATGTTGAGGA TGAGGAGACG TGGATTCGAG AGAAAGAGCC CATTGCCGCA 
TCTACCAACA GAGGTAAGGA TTTAATTGGG GTCCAGAATC TGCTAAAGAA ACATCAAGCC 
TTACAAGCAG AAATTGCTGG ACATGAACCA CGCATCAAAG CAGTTACACA GAAGGGGAAT 
GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAGCT TCACGAGCTG 
AACCAAAAGT GGGAGGCACT GAAAGCCAAA GCTTCCCAGC GTCGGCAGGA CCTGGAGGAC 
TCTCTGCAGG CCCAGCAGTA CTTTGCTGAT GCTAACGAGG CTGAATCCTG GATGCGGGAG 
AAGGAACCCA TTGTGGGCAG CACTGACTAT GGCAAGGACG AAGACTCTGC TGAGGCTCTA 
CTGAAGAAAC ACGAAGCTTT GATGTCAGAT CTCAGTGCCT ACGGCAGCAG CATCCAGGCT 
TTGCGAGAAC AAGCACAGTC CTGCCGGCAA CAAGTGGCCC CCACGGATGA TGAGACTGGG 
AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAGGGAG ATATCCTTAC CTTACTCAAC AGCACCAACA AGGATTGGTG GAAAGTGGAA 
GTGAACGATC GTCAGGGTTT TGTGCCGGCT GCGTACGTGA AGAAATTGGA CCCCGCCCAG 
TCAGCCTCCC GGGAGAATCT CCTGGAGGAG CAAGGCAGCA TAGCACTGCG GCAGGAGCAG 
ATTGACAATC AATATCATTC TCTGCTGGAA CTGGGTGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGTTGCA AGAAGTTTAT GTTGTTCCGT GAAGCGAATG AACTACAGCA ATGGATCAAT 
GAGAAGGAAG CCGCTCTGAC AAGTGAGGAG GTCGGAGCAG ACTTGGAGCA GGTTGAGGTG 
CTCCAGAAGA AGTTTGATGA CTTCCAGAAG GACCTGAAGG CCAATGAGTC ACGGTTGAAG 
GACATTAACA AGGTAGCTGA AGACCTGGAG TCTGAAGGTC TCATGGCAGA GGAGGTGCAG 
GCTGTGCAAC AACAGGAAGT GTATGGCATG ATGCCCAGGG ATGAAACTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGTCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTGA ATGAGCGCTG GCGGTCCCTA CAGCAGCTGG CCGAGGAACG GAGCCAGCTC 
TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CTGATGAAAC CAAAGAATGG 
ATTGAAGAGA AGAATCAAGC TCTAAACACA GACAATTATG GACATGATCT CGCCAGTGTC 
CAGGCCCTGC AACGCAAGCA TGAGGGCTTC GAGAGGGACC TTGCGGCTCT CGGTGACAAG 
GTAAACTCCC TTGGTGAAAC AGCAGAGCGC CTGATCCAGT CCCATCCCGA GTCAGCAGAA 
GACCTGCAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA GCAGCCTGGG GAAACGTGCA 
GATCAGCGCA AGGCAAAGTT GGGTGACTCC CACGACCTGC AGCGCTTCCT TAGCGATTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATA CGGGGGTTGG TGTCCTCAGA TGAGCTAGCC 
AAGGATGTCA CCGGAGCTGA GGCATTGCTG GAGCGACACC AGGAACACCG GACAGAAATC 
GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GACAGCAGCT GTTGGCTCAC 
GGACACTATG CCAGCCCTGA GATCAAGCAG AAACTTGATA TTCTTGACCA GGAGCGTGCA 
GACCTGGAGA AGGCCTGGGT TCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAACTGCAG 
CTGTTCCATC GGGACTGTGA GCAAGCTGAG AACTGGATGG CTGCCCGGGA GGCCTTCTTG 
AATACCGAAG ACAAAGGAGA CTCACTGGAC AGCGTAGAGG CTCTGATCAA AAAACATGAA 
GACTTTGACA AAGCGATTAA CGTCCAGGAA GAGAAGATTG CTGCTCTGCA GGCCTTTGCC 
GACCAGCTCA TCGCTGCCGG CCATTATGCC AAGGGAGACA TTTCTAGCCG GCGCAATGAG 
GTCTTGGACA GGTGGCGACG TCTGAAAGCC CAGATGATTG AGAAAAGGTC AAAGCTAGGA 
GAATCTCAAA CCCTCCAACA GTTCAGCCGG GATGTGGATG AGATTGAGGC TTGGATCAGT 
GAAAAATTGC AAACAGCGAG TGATGAGTCG TACAAGGATC CCACCAACAT CCAGCTTTCC 
AAGCTGCTGA GCAAGCACCA GAAGCACCAG GCTTTTGAAG CAGAGCTGCA TGCCAACGCT 
GACCGGATCC GTGGGGTTAT CGACATGGGC AACTCCCTCA TTGAACGTGG AGCCTGTGCC 
GGCAGTGAGG ATGCTGTCAA GGCCCGCCTG GCTGCCTTAG CTGACCAGTG GCAGTTCTTG 
GTGCAAAAGT CAGCGGAAAA GAGCCAGAAA CTGAAAGAAG CCAACAAGCA GCAGAACTTC 
AACACAGGGA TCAAGGACTT TGACTTCTGG CTGTCTGAGG TGGAGGCCCT GCTGGCATCC 
GAAGATTATG GCAAAGACCT AGCTTCTGTG AACAACCTGC TGAAAAAGCA TCAACTGCTG 
GAAGCAGATA TATCTGCCCA TGAGGATCGC CTGAAGGACC TGAACAGCCA GGCAGACAGC 
CTGATGACCA GCAGTGCCTT CGACACCTCC CAAGTAAAGG ACAAGAGGGA CACCATCAAC 
GGGCGCTTCC AGAAGATCAA GAGCATGGCG GCCTCCCGGC GAGCCAAGCT GAATGAATCC 
CATCGCCTGC ACCAGTTCTT CCGGGACATG GATGACGAGG AGTCCTGGAT CAAGGAGAAG 
AAGCTGCTGG TGGGCTCAGA GGACTACGGC CGGGACCTAA CCGGCGTGCA GAACCTGAGG 
AAGAAGCACA AGCGGCTGGA AGCAGAACTG GCTGCGCATG AGCCGGCTAT TCAGGGTGTC 
CTGGACACTG GCAAGAAGCT GTCCGATGAC AACACCATCG GGAAAGAGGA GATCCAGCAG 
CGGCTGGCGC AGTTTGTGGA GCACTGGAAA GAGCTGAAGC AGCTGGCAGC TGCCCGGGGT 
CAGCGGCTGG AAGAGTCCTT GGAATATCAG CAGTTTGTAG CCAATGTGGA AGAGGAAGAA 
GCCTGGATCA ATGAGAAAAT GACCCTGGTG GCCAGCGAAG ATTATGGCGA CACTCTTGCC 
GCCATCCAGG GCTTACTGAA GAAACATGAA GCTTTTGAGA CAGACTTCAC CGTCCACAAG 
GATCGCGTGA ATGATGTCTG CACCAATGGA CAAGACCTCA TTAAGAAGAA CAATCACCAT 
GAGGAGAACA TCTCTTCAAA GATGAAGGGC CTGAACGGGA AAGTGTCAGA CCTGGAGAAA 
GCTGCAGCCC AGAGAAAGGC GAAGCTGGAT GAGAACTCGG CCTTCCTTCA GTTCAACTGG 
AAGGCGGACG TGGTGGAGTC CTGGATCGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT 
TATGGCCGAG ACCTGTCTTC TGTGCAGACG CTCCTCACCA AACAGGAAAC TTTTGACGCT 
GGGCTGCAGG CCTTCCAGCA GGAAGGCATT GCCAACATCA CTGCCCTCAA AGATCAGCTT 
CTCGCCGCCA AACACGTTCA GTCCAAGGCC ATCGAGGCCC GGCACGCCTC CCTCATGAAG 
AGGTGGAGCC AGCTTCTGGC CAACTCAGCC GCCCGCAAGA AGAAGCTTCT GGAGGCTCAG 
AGTCACTTCC GCAAGGTGGA GGACCTCTTC CTGACCTTCG CCAAAAAGGC TTCTGCCTTC 
AACAGCTGGT TTGAAAATGC AGAGGAGGAC TTAACAGACC CCGTGCGCTG CAACTCCTTG 
GAAGAAATCA AAGCTTTGCG CGAGGCCCAC GACGCCTTCC GCTCCTCCCT CAGCTCTGCC 
CAGGCTGACT TCAACCAGCT GGCCGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTAGCC 
TCCAACCCCT ACACCTGGTT TACCATGGAG GCCCTGGAGG AGACCTGGAG GAACCTACAG 
AAAATCATCA AGGAGAGGGA GCTGGAGCTG CAGAAGGAAC AGCGGCGGCA GGAGGAGAAC 
GACAAGCTGC GCCAGGAGTT TGCCCAGCAC GCCAACGCCT TCCACCAGTG GATCCAAGAG 
ACCAGGACAT ACCTCCTCGA TGGGTCCTGT ATGGTGGAAG AGTCGGGGAC CCTCGAATCC 
CAGCTTGAAG CTACCAAACG CAAGCACCAG GAAATCCGAG CCATGAGAAG TCAGCTCAAA 
AAGATCGAGG ACCTGGGGGC CGCCATGGAG GAGGCCCTCA TCCTGGACAA CAAGTACACG 
GAGCACAGCA CCGTGGGCCT CGCCCAGCAG TGGGACCAGC TGGACCAGCT GGGCATGCGC 
ATGCAGCACA ACCTGGAGCA GCAGATCCAG GCCAGGAACA CAACAGGTGT GACTGAGGAG 
GCCCTCAAAG AATTCAGCAT GATGTTTAAA CACTTTGACA AGGACAAGTC TGGCAGGCTG 
AACCATCAGG AGTTCAAATC TTGCCTGCGC TCCCTGGGCT ATGACCTGCC CATGGTGGAG 
GAAGGGGAAC CTGACCCTGA GTTCGAGGCA ATCCTGGACA CGGTGGATCC GAACAGAGAT 
GGCCATGTCT CCTTGCAAGA ATACATGGCT TTCATGATCA GCCGCGAAAC TGAGAACGTC 
AAGTCCAGCG AGGAGATTGA GAGCGCCTTC CGGGCCCTCA GCTCAGAGGG AAAGCCTTAC 
GTGACCAAGG AGGAGCTCTA CCAGAACCTG ACCCGGGAAC AAGCCGACTA CTGCGTCTCC 
CACATGAAGC CCTACGTGGA CGGCAAGGGC CGCGAGCTCC CCACCGCGTT CGACTACGTG 
GAGTTCACCC GCTCGCTTTT CGTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006717315.1
CDS	14..7423
Translation	MLSSFRKRRVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELASDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMRRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPTDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGMMPRDETDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKQKLDILDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAAGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINGRFQKIKSMAASRRAKLNESHRLHQFFRDMDDEESWIKEKKLLVGSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHASLMKRWSQLLANSAARKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_006717252.2
Organism	Homo sapiens(human)
Definition	Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006717252.2

ORF Insert Sequence:

ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACCG ATACCACCGC 
TTCAAGGAAC TCTCAACCCT TAGGCGTCAG AAGCTGGAAG ATTCCTATCG ATTCCAGTTC 
TTTCAAAGAG ATGCTGAAGA GCTGGAGAAA TGGATACAGG AAAAACTTCA GATTGCATCT 
GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGAAAGC TTCAGAAGCA TCAAGCATTT 
GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAGC TGGATGAAAC TGGAAACCTG 
ATGATCTCAG AAGGGCATTT TGCATCTGAA ACCATACGGA CCCGTTTGAT GGAGCTGCAC 
CGCCAGTGGG AATTACTTTT GGAGAAGATG CGAGAAAAAG GAATCAAACT GCTGCAGGCC 
CAGAAGTTGG TGCAGTACTT ACGAGAATGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCAATTG TTACTTCTGA AGAGCTGGGC CAGGATCTGG AGCATGTAGA GGTTTTACAG 
AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCTCATG AAGAAAGAGT TAATGAAGTG 
AACCAGTTTG CTGCCAAACT CATACAGGAG CAGCACCCTG AGGAGGAACT GATCAAGACT 
AAGCAGGATG AAGTCAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCTCT GCAGAGGCAG 
GGGAAGCTCT TTGGGGCAGC AGAAGTTCAG CGCTTTAACA GGGATGTGGA TGAGACTATC 
AGTTGGATTA AGGAAAAGGA GCAGTTAATG GCCTCTGATG ATTTTGGCCG AGACCTGGCA 
AGTGTTCAGG CTCTGCTTCG GAAGCACGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAA 
GACAAGGTCA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCCCA CCCTCTGAGT 
GCAACACAGA TTCAAGTGAA GCGAGAGGAA CTGATTACAA ACTGGGAGCA GATCCGCACC 
TTGGCGGCAG AGAGACATGC ACGGCTCAAT GATTCATACA GGCTTCAACG CTTCCTTGCT 
GACTTCCGTG ACCTCACCAG CTGGGTGACT GAGATGAAAG CCCTCATCAA TGCAGATGAG 
CTTGCCAGTG ATGTGGCTGG GGCTGAAGCC CTGCTAGATA GACACCAAGA GCACAAGGGT 
GAAATTGATG CCCATGAAGA CAGCTTCAAA TCTGCAGATG AATCTGGACA GGCACTGCTT 
GCTGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCGTCCT TTCCGAGGAG 
AGAGCGGCGC TGCTGGAGCT GTGGGAGCTG CGCAGGCAGC AGTACGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGGGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ATGAAGACTT GGGAGATTCC TTGGATAGTG TGGAAGCGCT TCTTAAGAAG 
CACGAAGACT TTGAGAAATC CCTTAGTGCC CAGGAGGAAA AGATTACAGC ATTAGATGAA 
TTTGCAACCA AGCTAATTCA GAACAACCAC TATGCAATGG AAGATGTGGC CACTCGCCGA 
GATGCTCTGT TGAGCCGCCG CAATGCCCTT CACGAGAGAG CCATGCGTCG CCGGGCCCAG 
CTAGCCGATT CTTTCCATCT GCAGCAGTTT TTCCGTGATT CTGATGAGCT CAAGAGTTGG 
GTCAATGAGA AGATGAAAAC TGCCACAGAT GAAGCTTATA AAGATCCATC CAACCTACAA 
GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CAGCAAACCA GAGCCGAATT 
GATGCCTTGG AGAAAGCTGG CCAAAAGCTG ATTGATGTCA ACCACTATGC CAAGGATGAA 
GTGGCAGCTC GTATGAATGA GGTGATCAGT TTGTGGAAGA AACTGCTAGA GGCCACTGAA 
CTGAAAGGAA TAAAGCTTCG TGAAGCCAAC CAGCAACAGC AATTTAATCG CAATGTTGAG 
GATATTGAAT TGTGGCTATA TGAAGTAGAA GGTCACTTGG CTTCGGATGA TTACGGCAAA 
GATCTTACCA ATGTGCAGAA CCTCCAGAAG AAACATGCAC TGCTAGAGGC AGATGTGGCT 
GCTCACCAGG ACCGAATTGA TGGCATCACC ATTCAGGCCC GCCAGTTCCA AGATGCTGGC 
CATTTTGATG CAGAAAACAT CAAGAAGAAA CAGGAAGCCC TCGTGGCTCG CTATGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCTCTGCG GTTGCAGCAG 
CTCTTCCGGG ATGTTGAGGA TGAGGAGACG TGGATTCGAG AGAAAGAGCC CATTGCCGCA 
TCTACCAACA GAGGTAAGGA TTTAATTGGG GTCCAGAATC TGCTAAAGAA ACATCAAGCC 
TTACAAGCAG AAATTGCTGG ACATGAACCA CGCATCAAAG CAGTTACACA GAAGGGGAAT 
GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAGCT TCACGAGCTG 
AACCAAAAGT GGGAGGCACT GAAAGCCAAA GCTTCCCAGC GTCGGCAGGA CCTGGAGGAC 
TCTCTGCAGG CCCAGCAGTA CTTTGCTGAT GCTAACGAGG CTGAATCCTG GATGCGGGAG 
AAGGAACCCA TTGTGGGCAG CACTGACTAT GGCAAGGACG AAGACTCTGC TGAGGCTCTA 
CTGAAGAAAC ACGAAGCTTT GATGTCAGAT CTCAGTGCCT ACGGCAGCAG CATCCAGGCT 
TTGCGAGAAC AAGCACAGTC CTGCCGGCAA CAAGTGGCCC CCACGGATGA TGAGACTGGG 
AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAGGGAG ATATCCTTAC CTTACTCAAC AGCACCAACA AGGATTGGTG GAAAGTGGAA 
GTGAACGATC GTCAGGGTTT TGTGCCGGCT GCGTACGTGA AGAAATTGGA CCCCGCCCAG 
TCAGCCTCCC GGGAGAATCT CCTGGAGGAG CAAGGCAGCA TAGCACTGCG GCAGGAGCAG 
ATTGACAATC AATATCATTC TCTGCTGGAA CTGGGTGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGTTGCA AGAAGTTTAT GTTGTTCCGT GAAGCGAATG AACTACAGCA ATGGATCAAT 
GAGAAGGAAG CCGCTCTGAC AAGTGAGGAG GTCGGAGCAG ACTTGGAGCA GGTTGAGGTG 
CTCCAGAAGA AGTTTGATGA CTTCCAGAAG GACCTGAAGG CCAATGAGTC ACGGTTGAAG 
GACATTAACA AGGTAGCTGA AGACCTGGAG TCTGAAGGTC TCATGGCAGA GGAGGTGCAG 
GCTGTGCAAC AACAGGAAGT GTATGGCATG ATGCCCAGGG ATGAAACTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGTCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTGA ATGAGCGCTG GCGGTCCCTA CAGCAGCTGG CCGAGGAACG GAGCCAGCTC 
TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CTGATGAAAC CAAAGAATGG 
ATTGAAGAGA AGAATCAAGC TCTAAACACA GACAATTATG GACATGATCT CGCCAGTGTC 
CAGGCCCTGC AACGCAAGCA TGAGGGCTTC GAGAGGGACC TTGCGGCTCT CGGTGACAAG 
GTAAACTCCC TTGGTGAAAC AGCAGAGCGC CTGATCCAGT CCCATCCCGA GTCAGCAGAA 
GACCTGCAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA GCAGCCTGGG GAAACGTGCA 
GATCAGCGCA AGGCAAAGTT GGGTGACTCC CACGACCTGC AGCGCTTCCT TAGCGATTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATA CGGGGGTTGG TGTCCTCAGA TGAGCTAGCC 
AAGGATGTCA CCGGAGCTGA GGCATTGCTG GAGCGACACC AGGAACACCG GACAGAAATC 
GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GACAGCAGCT GTTGGCTCAC 
GGACACTATG CCAGCCCTGA GATCAAGCAG AAACTTGATA TTCTTGACCA GGAGCGTGCA 
GACCTGGAGA AGGCCTGGGT TCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAACTGCAG 
CTGTTCCATC GGGACTGTGA GCAAGCTGAG AACTGGATGG CTGCCCGGGA GGCCTTCTTG 
AATACCGAAG ACAAAGGAGA CTCACTGGAC AGCGTAGAGG CTCTGATCAA AAAACATGAA 
GACTTTGACA AAGCGATTAA CGTCCAGGAA GAGAAGATTG CTGCTCTGCA GGCCTTTGCC 
GACCAGCTCA TCGCTGCCGG CCATTATGCC AAGGGAGACA TTTCTAGCCG GCGCAATGAG 
GTCTTGGACA GGTGGCGACG TCTGAAAGCC CAGATGATTG AGAAAAGGTC AAAGCTAGGA 
GAATCTCAAA CCCTCCAACA GTTCAGCCGG GATGTGGATG AGATTGAGGC TTGGATCAGT 
GAAAAATTGC AAACAGCGAG TGATGAGTCG TACAAGGATC CCACCAACAT CCAGCTTTCC 
AAGCTGCTGA GCAAGCACCA GAAGCACCAG GCTTTTGAAG CAGAGCTGCA TGCCAACGCT 
GACCGGATCC GTGGGGTTAT CGACATGGGC AACTCCCTCA TTGAACGTGG AGCCTGTGCC 
GGCAGTGAGG ATGCTGTCAA GGCCCGCCTG GCTGCCTTAG CTGACCAGTG GCAGTTCTTG 
GTGCAAAAGT CAGCGGAAAA GAGCCAGAAA CTGAAAGAAG CCAACAAGCA GCAGAACTTC 
AACACAGGGA TCAAGGACTT TGACTTCTGG CTGTCTGAGG TGGAGGCCCT GCTGGCATCC 
GAAGATTATG GCAAAGACCT AGCTTCTGTG AACAACCTGC TGAAAAAGCA TCAACTGCTG 
GAAGCAGATA TATCTGCCCA TGAGGATCGC CTGAAGGACC TGAACAGCCA GGCAGACAGC 
CTGATGACCA GCAGTGCCTT CGACACCTCC CAAGTAAAGG ACAAGAGGGA CACCATCAAC 
GGGCGCTTCC AGAAGATCAA GAGCATGGCG GCCTCCCGGC GAGCCAAGCT GAATGAATCC 
CATCGCCTGC ACCAGTTCTT CCGGGACATG GATGACGAGG AGTCCTGGAT CAAGGAGAAG 
AAGCTGCTGG TGGGCTCAGA GGACTACGGC CGGGACCTAA CCGGCGTGCA GAACCTGAGG 
AAGAAGCACA AGCGGCTGGA AGCAGAACTG GCTGCGCATG AGCCGGCTAT TCAGGGTGTC 
CTGGACACTG GCAAGAAGCT GTCCGATGAC AACACCATCG GGAAAGAGGA GATCCAGCAG 
CGGCTGGCGC AGTTTGTGGA GCACTGGAAA GAGCTGAAGC AGCTGGCAGC TGCCCGGGGT 
CAGCGGCTGG AAGAGTCCTT GGAATATCAG CAGTTTGTAG CCAATGTGGA AGAGGAAGAA 
GCCTGGATCA ATGAGAAAAT GACCCTGGTG GCCAGCGAAG ATTATGGCGA CACTCTTGCC 
GCCATCCAGG GCTTACTGAA GAAACATGAA GCTTTTGAGA CAGACTTCAC CGTCCACAAG 
GATCGCGTGA ATGATGTCTG CACCAATGGA CAAGACCTCA TTAAGAAGAA CAATCACCAT 
GAGGAGAACA TCTCTTCAAA GATGAAGGGC CTGAACGGGA AAGTGTCAGA CCTGGAGAAA 
GCTGCAGCCC AGAGAAAGGC GAAGCTGGAT GAGAACTCGG CCTTCCTTCA GTTCAACTGG 
AAGGCGGACG TGGTGGAGTC CTGGATCGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT 
TATGGCCGAG ACCTGTCTTC TGTGCAGACG CTCCTCACCA AACAGGAAAC TTTTGACGCT 
GGGCTGCAGG CCTTCCAGCA GGAAGGCATT GCCAACATCA CTGCCCTCAA AGATCAGCTT 
CTCGCCGCCA AACACGTTCA GTCCAAGGCC ATCGAGGCCC GGCACGCCTC CCTCATGAAG 
AGGTGGAGCC AGCTTCTGGC CAACTCAGCC GCCCGCAAGA AGAAGCTTCT GGAGGCTCAG 
AGTCACTTCC GCAAGGTGGA GGACCTCTTC CTGACCTTCG CCAAAAAGGC TTCTGCCTTC 
AACAGCTGGT TTGAAAATGC AGAGGAGGAC TTAACAGACC CCGTGCGCTG CAACTCCTTG 
GAAGAAATCA AAGCTTTGCG CGAGGCCCAC GACGCCTTCC GCTCCTCCCT CAGCTCTGCC 
CAGGCTGACT TCAACCAGCT GGCCGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTAGCC 
TCCAACCCCT ACACCTGGTT TACCATGGAG GCCCTGGAGG AGACCTGGAG GAACCTACAG 
AAAATCATCA AGGAGAGGGA GCTGGAGCTG CAGAAGGAAC AGCGGCGGCA GGAGGAGAAC 
GACAAGCTGC GCCAGGAGTT TGCCCAGCAC GCCAACGCCT TCCACCAGTG GATCCAAGAG 
ACCAGGACAT ACCTCCTCGA TGGGTCCTGT ATGGTGGAAG AGTCGGGGAC CCTCGAATCC 
CAGCTTGAAG CTACCAAACG CAAGCACCAG GAAATCCGAG CCATGAGAAG TCAGCTCAAA 
AAGATCGAGG ACCTGGGGGC CGCCATGGAG GAGGCCCTCA TCCTGGACAA CAAGTACACG 
GAGCACAGCA CCGTGGGCCT CGCCCAGCAG TGGGACCAGC TGGACCAGCT GGGCATGCGC 
ATGCAGCACA ACCTGGAGCA GCAGATCCAG GCCAGGAACA CAACAGGTGT GACTGAGGAG 
GCCCTCAAAG AATTCAGCAT GATGTTTAAA CACTTTGACA AGGACAAGTC TGGCAGGCTG 
AACCATCAGG AGTTCAAATC TTGCCTGCGC TCCCTGGGCT ATGACCTGCC CATGGTGGAG 
GAAGGGGAAC CTGACCCTGA GTTCGAGGCA ATCCTGGACA CGGTGGATCC GAACAGAGAT 
GGCCATGTCT CCTTGCAAGA ATACATGGCT TTCATGATCA GCCGCGAAAC TGAGAACGTC 
AAGTCCAGCG AGGAGATTGA GAGCGCCTTC CGGGCCCTCA GCTCAGAGGG AAAGCCTTAC 
GTGACCAAGG AGGAGCTCTA CCAGAACCTG ACCCGGGAAC AAGCCGACTA CTGCGTCTCC 
CACATGAAGC CCTACGTGGA CGGCAAGGGC CGCGAGCTCC CCACCGCGTT CGACTACGTG 
GAGTTCACCC GCTCGCTTTT CGTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_006717252.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006717315.1 Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X10, mRNA.

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CloneID	OHu43460
Clone ID Related Accession (Same CDS sequence)	XM_006717252.2 , XM_006717252.3
Accession Version	XM_006717252.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7410bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006717252.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACCG ATACCACCGC 
TTCAAGGAAC TCTCAACCCT TAGGCGTCAG AAGCTGGAAG ATTCCTATCG ATTCCAGTTC 
TTTCAAAGAG ATGCTGAAGA GCTGGAGAAA TGGATACAGG AAAAACTTCA GATTGCATCT 
GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGAAAGC TTCAGAAGCA TCAAGCATTT 
GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAGC TGGATGAAAC TGGAAACCTG 
ATGATCTCAG AAGGGCATTT TGCATCTGAA ACCATACGGA CCCGTTTGAT GGAGCTGCAC 
CGCCAGTGGG AATTACTTTT GGAGAAGATG CGAGAAAAAG GAATCAAACT GCTGCAGGCC 
CAGAAGTTGG TGCAGTACTT ACGAGAATGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCAATTG TTACTTCTGA AGAGCTGGGC CAGGATCTGG AGCATGTAGA GGTTTTACAG 
AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCTCATG AAGAAAGAGT TAATGAAGTG 
AACCAGTTTG CTGCCAAACT CATACAGGAG CAGCACCCTG AGGAGGAACT GATCAAGACT 
AAGCAGGATG AAGTCAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCTCT GCAGAGGCAG 
GGGAAGCTCT TTGGGGCAGC AGAAGTTCAG CGCTTTAACA GGGATGTGGA TGAGACTATC 
AGTTGGATTA AGGAAAAGGA GCAGTTAATG GCCTCTGATG ATTTTGGCCG AGACCTGGCA 
AGTGTTCAGG CTCTGCTTCG GAAGCACGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAA 
GACAAGGTCA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCCCA CCCTCTGAGT 
GCAACACAGA TTCAAGTGAA GCGAGAGGAA CTGATTACAA ACTGGGAGCA GATCCGCACC 
TTGGCGGCAG AGAGACATGC ACGGCTCAAT GATTCATACA GGCTTCAACG CTTCCTTGCT 
GACTTCCGTG ACCTCACCAG CTGGGTGACT GAGATGAAAG CCCTCATCAA TGCAGATGAG 
CTTGCCAGTG ATGTGGCTGG GGCTGAAGCC CTGCTAGATA GACACCAAGA GCACAAGGGT 
GAAATTGATG CCCATGAAGA CAGCTTCAAA TCTGCAGATG AATCTGGACA GGCACTGCTT 
GCTGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCGTCCT TTCCGAGGAG 
AGAGCGGCGC TGCTGGAGCT GTGGGAGCTG CGCAGGCAGC AGTACGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGGGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ATGAAGACTT GGGAGATTCC TTGGATAGTG TGGAAGCGCT TCTTAAGAAG 
CACGAAGACT TTGAGAAATC CCTTAGTGCC CAGGAGGAAA AGATTACAGC ATTAGATGAA 
TTTGCAACCA AGCTAATTCA GAACAACCAC TATGCAATGG AAGATGTGGC CACTCGCCGA 
GATGCTCTGT TGAGCCGCCG CAATGCCCTT CACGAGAGAG CCATGCGTCG CCGGGCCCAG 
CTAGCCGATT CTTTCCATCT GCAGCAGTTT TTCCGTGATT CTGATGAGCT CAAGAGTTGG 
GTCAATGAGA AGATGAAAAC TGCCACAGAT GAAGCTTATA AAGATCCATC CAACCTACAA 
GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CAGCAAACCA GAGCCGAATT 
GATGCCTTGG AGAAAGCTGG CCAAAAGCTG ATTGATGTCA ACCACTATGC CAAGGATGAA 
GTGGCAGCTC GTATGAATGA GGTGATCAGT TTGTGGAAGA AACTGCTAGA GGCCACTGAA 
CTGAAAGGAA TAAAGCTTCG TGAAGCCAAC CAGCAACAGC AATTTAATCG CAATGTTGAG 
GATATTGAAT TGTGGCTATA TGAAGTAGAA GGTCACTTGG CTTCGGATGA TTACGGCAAA 
GATCTTACCA ATGTGCAGAA CCTCCAGAAG AAACATGCAC TGCTAGAGGC AGATGTGGCT 
GCTCACCAGG ACCGAATTGA TGGCATCACC ATTCAGGCCC GCCAGTTCCA AGATGCTGGC 
CATTTTGATG CAGAAAACAT CAAGAAGAAA CAGGAAGCCC TCGTGGCTCG CTATGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCTCTGCG GTTGCAGCAG 
CTCTTCCGGG ATGTTGAGGA TGAGGAGACG TGGATTCGAG AGAAAGAGCC CATTGCCGCA 
TCTACCAACA GAGGTAAGGA TTTAATTGGG GTCCAGAATC TGCTAAAGAA ACATCAAGCC 
TTACAAGCAG AAATTGCTGG ACATGAACCA CGCATCAAAG CAGTTACACA GAAGGGGAAT 
GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAGCT TCACGAGCTG 
AACCAAAAGT GGGAGGCACT GAAAGCCAAA GCTTCCCAGC GTCGGCAGGA CCTGGAGGAC 
TCTCTGCAGG CCCAGCAGTA CTTTGCTGAT GCTAACGAGG CTGAATCCTG GATGCGGGAG 
AAGGAACCCA TTGTGGGCAG CACTGACTAT GGCAAGGACG AAGACTCTGC TGAGGCTCTA 
CTGAAGAAAC ACGAAGCTTT GATGTCAGAT CTCAGTGCCT ACGGCAGCAG CATCCAGGCT 
TTGCGAGAAC AAGCACAGTC CTGCCGGCAA CAAGTGGCCC CCACGGATGA TGAGACTGGG 
AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAGGGAG ATATCCTTAC CTTACTCAAC AGCACCAACA AGGATTGGTG GAAAGTGGAA 
GTGAACGATC GTCAGGGTTT TGTGCCGGCT GCGTACGTGA AGAAATTGGA CCCCGCCCAG 
TCAGCCTCCC GGGAGAATCT CCTGGAGGAG CAAGGCAGCA TAGCACTGCG GCAGGAGCAG 
ATTGACAATC AATATCATTC TCTGCTGGAA CTGGGTGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGTTGCA AGAAGTTTAT GTTGTTCCGT GAAGCGAATG AACTACAGCA ATGGATCAAT 
GAGAAGGAAG CCGCTCTGAC AAGTGAGGAG GTCGGAGCAG ACTTGGAGCA GGTTGAGGTG 
CTCCAGAAGA AGTTTGATGA CTTCCAGAAG GACCTGAAGG CCAATGAGTC ACGGTTGAAG 
GACATTAACA AGGTAGCTGA AGACCTGGAG TCTGAAGGTC TCATGGCAGA GGAGGTGCAG 
GCTGTGCAAC AACAGGAAGT GTATGGCATG ATGCCCAGGG ATGAAACTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGTCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTGA ATGAGCGCTG GCGGTCCCTA CAGCAGCTGG CCGAGGAACG GAGCCAGCTC 
TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CTGATGAAAC CAAAGAATGG 
ATTGAAGAGA AGAATCAAGC TCTAAACACA GACAATTATG GACATGATCT CGCCAGTGTC 
CAGGCCCTGC AACGCAAGCA TGAGGGCTTC GAGAGGGACC TTGCGGCTCT CGGTGACAAG 
GTAAACTCCC TTGGTGAAAC AGCAGAGCGC CTGATCCAGT CCCATCCCGA GTCAGCAGAA 
GACCTGCAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA GCAGCCTGGG GAAACGTGCA 
GATCAGCGCA AGGCAAAGTT GGGTGACTCC CACGACCTGC AGCGCTTCCT TAGCGATTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATA CGGGGGTTGG TGTCCTCAGA TGAGCTAGCC 
AAGGATGTCA CCGGAGCTGA GGCATTGCTG GAGCGACACC AGGAACACCG GACAGAAATC 
GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GACAGCAGCT GTTGGCTCAC 
GGACACTATG CCAGCCCTGA GATCAAGCAG AAACTTGATA TTCTTGACCA GGAGCGTGCA 
GACCTGGAGA AGGCCTGGGT TCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAACTGCAG 
CTGTTCCATC GGGACTGTGA GCAAGCTGAG AACTGGATGG CTGCCCGGGA GGCCTTCTTG 
AATACCGAAG ACAAAGGAGA CTCACTGGAC AGCGTAGAGG CTCTGATCAA AAAACATGAA 
GACTTTGACA AAGCGATTAA CGTCCAGGAA GAGAAGATTG CTGCTCTGCA GGCCTTTGCC 
GACCAGCTCA TCGCTGCCGG CCATTATGCC AAGGGAGACA TTTCTAGCCG GCGCAATGAG 
GTCTTGGACA GGTGGCGACG TCTGAAAGCC CAGATGATTG AGAAAAGGTC AAAGCTAGGA 
GAATCTCAAA CCCTCCAACA GTTCAGCCGG GATGTGGATG AGATTGAGGC TTGGATCAGT 
GAAAAATTGC AAACAGCGAG TGATGAGTCG TACAAGGATC CCACCAACAT CCAGCTTTCC 
AAGCTGCTGA GCAAGCACCA GAAGCACCAG GCTTTTGAAG CAGAGCTGCA TGCCAACGCT 
GACCGGATCC GTGGGGTTAT CGACATGGGC AACTCCCTCA TTGAACGTGG AGCCTGTGCC 
GGCAGTGAGG ATGCTGTCAA GGCCCGCCTG GCTGCCTTAG CTGACCAGTG GCAGTTCTTG 
GTGCAAAAGT CAGCGGAAAA GAGCCAGAAA CTGAAAGAAG CCAACAAGCA GCAGAACTTC 
AACACAGGGA TCAAGGACTT TGACTTCTGG CTGTCTGAGG TGGAGGCCCT GCTGGCATCC 
GAAGATTATG GCAAAGACCT AGCTTCTGTG AACAACCTGC TGAAAAAGCA TCAACTGCTG 
GAAGCAGATA TATCTGCCCA TGAGGATCGC CTGAAGGACC TGAACAGCCA GGCAGACAGC 
CTGATGACCA GCAGTGCCTT CGACACCTCC CAAGTAAAGG ACAAGAGGGA CACCATCAAC 
GGGCGCTTCC AGAAGATCAA GAGCATGGCG GCCTCCCGGC GAGCCAAGCT GAATGAATCC 
CATCGCCTGC ACCAGTTCTT CCGGGACATG GATGACGAGG AGTCCTGGAT CAAGGAGAAG 
AAGCTGCTGG TGGGCTCAGA GGACTACGGC CGGGACCTAA CCGGCGTGCA GAACCTGAGG 
AAGAAGCACA AGCGGCTGGA AGCAGAACTG GCTGCGCATG AGCCGGCTAT TCAGGGTGTC 
CTGGACACTG GCAAGAAGCT GTCCGATGAC AACACCATCG GGAAAGAGGA GATCCAGCAG 
CGGCTGGCGC AGTTTGTGGA GCACTGGAAA GAGCTGAAGC AGCTGGCAGC TGCCCGGGGT 
CAGCGGCTGG AAGAGTCCTT GGAATATCAG CAGTTTGTAG CCAATGTGGA AGAGGAAGAA 
GCCTGGATCA ATGAGAAAAT GACCCTGGTG GCCAGCGAAG ATTATGGCGA CACTCTTGCC 
GCCATCCAGG GCTTACTGAA GAAACATGAA GCTTTTGAGA CAGACTTCAC CGTCCACAAG 
GATCGCGTGA ATGATGTCTG CACCAATGGA CAAGACCTCA TTAAGAAGAA CAATCACCAT 
GAGGAGAACA TCTCTTCAAA GATGAAGGGC CTGAACGGGA AAGTGTCAGA CCTGGAGAAA 
GCTGCAGCCC AGAGAAAGGC GAAGCTGGAT GAGAACTCGG CCTTCCTTCA GTTCAACTGG 
AAGGCGGACG TGGTGGAGTC CTGGATCGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT 
TATGGCCGAG ACCTGTCTTC TGTGCAGACG CTCCTCACCA AACAGGAAAC TTTTGACGCT 
GGGCTGCAGG CCTTCCAGCA GGAAGGCATT GCCAACATCA CTGCCCTCAA AGATCAGCTT 
CTCGCCGCCA AACACGTTCA GTCCAAGGCC ATCGAGGCCC GGCACGCCTC CCTCATGAAG 
AGGTGGAGCC AGCTTCTGGC CAACTCAGCC GCCCGCAAGA AGAAGCTTCT GGAGGCTCAG 
AGTCACTTCC GCAAGGTGGA GGACCTCTTC CTGACCTTCG CCAAAAAGGC TTCTGCCTTC 
AACAGCTGGT TTGAAAATGC AGAGGAGGAC TTAACAGACC CCGTGCGCTG CAACTCCTTG 
GAAGAAATCA AAGCTTTGCG CGAGGCCCAC GACGCCTTCC GCTCCTCCCT CAGCTCTGCC 
CAGGCTGACT TCAACCAGCT GGCCGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTAGCC 
TCCAACCCCT ACACCTGGTT TACCATGGAG GCCCTGGAGG AGACCTGGAG GAACCTACAG 
AAAATCATCA AGGAGAGGGA GCTGGAGCTG CAGAAGGAAC AGCGGCGGCA GGAGGAGAAC 
GACAAGCTGC GCCAGGAGTT TGCCCAGCAC GCCAACGCCT TCCACCAGTG GATCCAAGAG 
ACCAGGACAT ACCTCCTCGA TGGGTCCTGT ATGGTGGAAG AGTCGGGGAC CCTCGAATCC 
CAGCTTGAAG CTACCAAACG CAAGCACCAG GAAATCCGAG CCATGAGAAG TCAGCTCAAA 
AAGATCGAGG ACCTGGGGGC CGCCATGGAG GAGGCCCTCA TCCTGGACAA CAAGTACACG 
GAGCACAGCA CCGTGGGCCT CGCCCAGCAG TGGGACCAGC TGGACCAGCT GGGCATGCGC 
ATGCAGCACA ACCTGGAGCA GCAGATCCAG GCCAGGAACA CAACAGGTGT GACTGAGGAG 
GCCCTCAAAG AATTCAGCAT GATGTTTAAA CACTTTGACA AGGACAAGTC TGGCAGGCTG 
AACCATCAGG AGTTCAAATC TTGCCTGCGC TCCCTGGGCT ATGACCTGCC CATGGTGGAG 
GAAGGGGAAC CTGACCCTGA GTTCGAGGCA ATCCTGGACA CGGTGGATCC GAACAGAGAT 
GGCCATGTCT CCTTGCAAGA ATACATGGCT TTCATGATCA GCCGCGAAAC TGAGAACGTC 
AAGTCCAGCG AGGAGATTGA GAGCGCCTTC CGGGCCCTCA GCTCAGAGGG AAAGCCTTAC 
GTGACCAAGG AGGAGCTCTA CCAGAACCTG ACCCGGGAAC AAGCCGACTA CTGCGTCTCC 
CACATGAAGC CCTACGTGGA CGGCAAGGGC CGCGAGCTCC CCACCGCGTT CGACTACGTG 
GAGTTCACCC GCTCGCTTTT CGTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006717315.1
CDS	14..7423
Translation	MLSSFRKRRVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELASDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMRRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPTDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGMMPRDETDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKQKLDILDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAAGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINGRFQKIKSMAASRRAKLNESHRLHQFFRDMDDEESWIKEKKLLVGSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHASLMKRWSQLLANSAARKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_006717252.3
Organism	Homo sapiens(human)
Definition	Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006717252.3

ORF Insert Sequence:

ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACCG ATACCACCGC 
TTCAAGGAAC TCTCAACCCT TAGGCGTCAG AAGCTGGAAG ATTCCTATCG ATTCCAGTTC 
TTTCAAAGAG ATGCTGAAGA GCTGGAGAAA TGGATACAGG AAAAACTTCA GATTGCATCT 
GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGAAAGC TTCAGAAGCA TCAAGCATTT 
GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAGC TGGATGAAAC TGGAAACCTG 
ATGATCTCAG AAGGGCATTT TGCATCTGAA ACCATACGGA CCCGTTTGAT GGAGCTGCAC 
CGCCAGTGGG AATTACTTTT GGAGAAGATG CGAGAAAAAG GAATCAAACT GCTGCAGGCC 
CAGAAGTTGG TGCAGTACTT ACGAGAATGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCAATTG TTACTTCTGA AGAGCTGGGC CAGGATCTGG AGCATGTAGA GGTTTTACAG 
AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCTCATG AAGAAAGAGT TAATGAAGTG 
AACCAGTTTG CTGCCAAACT CATACAGGAG CAGCACCCTG AGGAGGAACT GATCAAGACT 
AAGCAGGATG AAGTCAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCTCT GCAGAGGCAG 
GGGAAGCTCT TTGGGGCAGC AGAAGTTCAG CGCTTTAACA GGGATGTGGA TGAGACTATC 
AGTTGGATTA AGGAAAAGGA GCAGTTAATG GCCTCTGATG ATTTTGGCCG AGACCTGGCA 
AGTGTTCAGG CTCTGCTTCG GAAGCACGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAA 
GACAAGGTCA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCCCA CCCTCTGAGT 
GCAACACAGA TTCAAGTGAA GCGAGAGGAA CTGATTACAA ACTGGGAGCA GATCCGCACC 
TTGGCGGCAG AGAGACATGC ACGGCTCAAT GATTCATACA GGCTTCAACG CTTCCTTGCT 
GACTTCCGTG ACCTCACCAG CTGGGTGACT GAGATGAAAG CCCTCATCAA TGCAGATGAG 
CTTGCCAGTG ATGTGGCTGG GGCTGAAGCC CTGCTAGATA GACACCAAGA GCACAAGGGT 
GAAATTGATG CCCATGAAGA CAGCTTCAAA TCTGCAGATG AATCTGGACA GGCACTGCTT 
GCTGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCGTCCT TTCCGAGGAG 
AGAGCGGCGC TGCTGGAGCT GTGGGAGCTG CGCAGGCAGC AGTACGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGGGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ATGAAGACTT GGGAGATTCC TTGGATAGTG TGGAAGCGCT TCTTAAGAAG 
CACGAAGACT TTGAGAAATC CCTTAGTGCC CAGGAGGAAA AGATTACAGC ATTAGATGAA 
TTTGCAACCA AGCTAATTCA GAACAACCAC TATGCAATGG AAGATGTGGC CACTCGCCGA 
GATGCTCTGT TGAGCCGCCG CAATGCCCTT CACGAGAGAG CCATGCGTCG CCGGGCCCAG 
CTAGCCGATT CTTTCCATCT GCAGCAGTTT TTCCGTGATT CTGATGAGCT CAAGAGTTGG 
GTCAATGAGA AGATGAAAAC TGCCACAGAT GAAGCTTATA AAGATCCATC CAACCTACAA 
GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CAGCAAACCA GAGCCGAATT 
GATGCCTTGG AGAAAGCTGG CCAAAAGCTG ATTGATGTCA ACCACTATGC CAAGGATGAA 
GTGGCAGCTC GTATGAATGA GGTGATCAGT TTGTGGAAGA AACTGCTAGA GGCCACTGAA 
CTGAAAGGAA TAAAGCTTCG TGAAGCCAAC CAGCAACAGC AATTTAATCG CAATGTTGAG 
GATATTGAAT TGTGGCTATA TGAAGTAGAA GGTCACTTGG CTTCGGATGA TTACGGCAAA 
GATCTTACCA ATGTGCAGAA CCTCCAGAAG AAACATGCAC TGCTAGAGGC AGATGTGGCT 
GCTCACCAGG ACCGAATTGA TGGCATCACC ATTCAGGCCC GCCAGTTCCA AGATGCTGGC 
CATTTTGATG CAGAAAACAT CAAGAAGAAA CAGGAAGCCC TCGTGGCTCG CTATGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCTCTGCG GTTGCAGCAG 
CTCTTCCGGG ATGTTGAGGA TGAGGAGACG TGGATTCGAG AGAAAGAGCC CATTGCCGCA 
TCTACCAACA GAGGTAAGGA TTTAATTGGG GTCCAGAATC TGCTAAAGAA ACATCAAGCC 
TTACAAGCAG AAATTGCTGG ACATGAACCA CGCATCAAAG CAGTTACACA GAAGGGGAAT 
GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAGCT TCACGAGCTG 
AACCAAAAGT GGGAGGCACT GAAAGCCAAA GCTTCCCAGC GTCGGCAGGA CCTGGAGGAC 
TCTCTGCAGG CCCAGCAGTA CTTTGCTGAT GCTAACGAGG CTGAATCCTG GATGCGGGAG 
AAGGAACCCA TTGTGGGCAG CACTGACTAT GGCAAGGACG AAGACTCTGC TGAGGCTCTA 
CTGAAGAAAC ACGAAGCTTT GATGTCAGAT CTCAGTGCCT ACGGCAGCAG CATCCAGGCT 
TTGCGAGAAC AAGCACAGTC CTGCCGGCAA CAAGTGGCCC CCACGGATGA TGAGACTGGG 
AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAGGGAG ATATCCTTAC CTTACTCAAC AGCACCAACA AGGATTGGTG GAAAGTGGAA 
GTGAACGATC GTCAGGGTTT TGTGCCGGCT GCGTACGTGA AGAAATTGGA CCCCGCCCAG 
TCAGCCTCCC GGGAGAATCT CCTGGAGGAG CAAGGCAGCA TAGCACTGCG GCAGGAGCAG 
ATTGACAATC AATATCATTC TCTGCTGGAA CTGGGTGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGTTGCA AGAAGTTTAT GTTGTTCCGT GAAGCGAATG AACTACAGCA ATGGATCAAT 
GAGAAGGAAG CCGCTCTGAC AAGTGAGGAG GTCGGAGCAG ACTTGGAGCA GGTTGAGGTG 
CTCCAGAAGA AGTTTGATGA CTTCCAGAAG GACCTGAAGG CCAATGAGTC ACGGTTGAAG 
GACATTAACA AGGTAGCTGA AGACCTGGAG TCTGAAGGTC TCATGGCAGA GGAGGTGCAG 
GCTGTGCAAC AACAGGAAGT GTATGGCATG ATGCCCAGGG ATGAAACTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGTCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTGA ATGAGCGCTG GCGGTCCCTA CAGCAGCTGG CCGAGGAACG GAGCCAGCTC 
TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CTGATGAAAC CAAAGAATGG 
ATTGAAGAGA AGAATCAAGC TCTAAACACA GACAATTATG GACATGATCT CGCCAGTGTC 
CAGGCCCTGC AACGCAAGCA TGAGGGCTTC GAGAGGGACC TTGCGGCTCT CGGTGACAAG 
GTAAACTCCC TTGGTGAAAC AGCAGAGCGC CTGATCCAGT CCCATCCCGA GTCAGCAGAA 
GACCTGCAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA GCAGCCTGGG GAAACGTGCA 
GATCAGCGCA AGGCAAAGTT GGGTGACTCC CACGACCTGC AGCGCTTCCT TAGCGATTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATA CGGGGGTTGG TGTCCTCAGA TGAGCTAGCC 
AAGGATGTCA CCGGAGCTGA GGCATTGCTG GAGCGACACC AGGAACACCG GACAGAAATC 
GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GACAGCAGCT GTTGGCTCAC 
GGACACTATG CCAGCCCTGA GATCAAGCAG AAACTTGATA TTCTTGACCA GGAGCGTGCA 
GACCTGGAGA AGGCCTGGGT TCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAACTGCAG 
CTGTTCCATC GGGACTGTGA GCAAGCTGAG AACTGGATGG CTGCCCGGGA GGCCTTCTTG 
AATACCGAAG ACAAAGGAGA CTCACTGGAC AGCGTAGAGG CTCTGATCAA AAAACATGAA 
GACTTTGACA AAGCGATTAA CGTCCAGGAA GAGAAGATTG CTGCTCTGCA GGCCTTTGCC 
GACCAGCTCA TCGCTGCCGG CCATTATGCC AAGGGAGACA TTTCTAGCCG GCGCAATGAG 
GTCTTGGACA GGTGGCGACG TCTGAAAGCC CAGATGATTG AGAAAAGGTC AAAGCTAGGA 
GAATCTCAAA CCCTCCAACA GTTCAGCCGG GATGTGGATG AGATTGAGGC TTGGATCAGT 
GAAAAATTGC AAACAGCGAG TGATGAGTCG TACAAGGATC CCACCAACAT CCAGCTTTCC 
AAGCTGCTGA GCAAGCACCA GAAGCACCAG GCTTTTGAAG CAGAGCTGCA TGCCAACGCT 
GACCGGATCC GTGGGGTTAT CGACATGGGC AACTCCCTCA TTGAACGTGG AGCCTGTGCC 
GGCAGTGAGG ATGCTGTCAA GGCCCGCCTG GCTGCCTTAG CTGACCAGTG GCAGTTCTTG 
GTGCAAAAGT CAGCGGAAAA GAGCCAGAAA CTGAAAGAAG CCAACAAGCA GCAGAACTTC 
AACACAGGGA TCAAGGACTT TGACTTCTGG CTGTCTGAGG TGGAGGCCCT GCTGGCATCC 
GAAGATTATG GCAAAGACCT AGCTTCTGTG AACAACCTGC TGAAAAAGCA TCAACTGCTG 
GAAGCAGATA TATCTGCCCA TGAGGATCGC CTGAAGGACC TGAACAGCCA GGCAGACAGC 
CTGATGACCA GCAGTGCCTT CGACACCTCC CAAGTAAAGG ACAAGAGGGA CACCATCAAC 
GGGCGCTTCC AGAAGATCAA GAGCATGGCG GCCTCCCGGC GAGCCAAGCT GAATGAATCC 
CATCGCCTGC ACCAGTTCTT CCGGGACATG GATGACGAGG AGTCCTGGAT CAAGGAGAAG 
AAGCTGCTGG TGGGCTCAGA GGACTACGGC CGGGACCTAA CCGGCGTGCA GAACCTGAGG 
AAGAAGCACA AGCGGCTGGA AGCAGAACTG GCTGCGCATG AGCCGGCTAT TCAGGGTGTC 
CTGGACACTG GCAAGAAGCT GTCCGATGAC AACACCATCG GGAAAGAGGA GATCCAGCAG 
CGGCTGGCGC AGTTTGTGGA GCACTGGAAA GAGCTGAAGC AGCTGGCAGC TGCCCGGGGT 
CAGCGGCTGG AAGAGTCCTT GGAATATCAG CAGTTTGTAG CCAATGTGGA AGAGGAAGAA 
GCCTGGATCA ATGAGAAAAT GACCCTGGTG GCCAGCGAAG ATTATGGCGA CACTCTTGCC 
GCCATCCAGG GCTTACTGAA GAAACATGAA GCTTTTGAGA CAGACTTCAC CGTCCACAAG 
GATCGCGTGA ATGATGTCTG CACCAATGGA CAAGACCTCA TTAAGAAGAA CAATCACCAT 
GAGGAGAACA TCTCTTCAAA GATGAAGGGC CTGAACGGGA AAGTGTCAGA CCTGGAGAAA 
GCTGCAGCCC AGAGAAAGGC GAAGCTGGAT GAGAACTCGG CCTTCCTTCA GTTCAACTGG 
AAGGCGGACG TGGTGGAGTC CTGGATCGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT 
TATGGCCGAG ACCTGTCTTC TGTGCAGACG CTCCTCACCA AACAGGAAAC TTTTGACGCT 
GGGCTGCAGG CCTTCCAGCA GGAAGGCATT GCCAACATCA CTGCCCTCAA AGATCAGCTT 
CTCGCCGCCA AACACGTTCA GTCCAAGGCC ATCGAGGCCC GGCACGCCTC CCTCATGAAG 
AGGTGGAGCC AGCTTCTGGC CAACTCAGCC GCCCGCAAGA AGAAGCTTCT GGAGGCTCAG 
AGTCACTTCC GCAAGGTGGA GGACCTCTTC CTGACCTTCG CCAAAAAGGC TTCTGCCTTC 
AACAGCTGGT TTGAAAATGC AGAGGAGGAC TTAACAGACC CCGTGCGCTG CAACTCCTTG 
GAAGAAATCA AAGCTTTGCG CGAGGCCCAC GACGCCTTCC GCTCCTCCCT CAGCTCTGCC 
CAGGCTGACT TCAACCAGCT GGCCGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTAGCC 
TCCAACCCCT ACACCTGGTT TACCATGGAG GCCCTGGAGG AGACCTGGAG GAACCTACAG 
AAAATCATCA AGGAGAGGGA GCTGGAGCTG CAGAAGGAAC AGCGGCGGCA GGAGGAGAAC 
GACAAGCTGC GCCAGGAGTT TGCCCAGCAC GCCAACGCCT TCCACCAGTG GATCCAAGAG 
ACCAGGACAT ACCTCCTCGA TGGGTCCTGT ATGGTGGAAG AGTCGGGGAC CCTCGAATCC 
CAGCTTGAAG CTACCAAACG CAAGCACCAG GAAATCCGAG CCATGAGAAG TCAGCTCAAA 
AAGATCGAGG ACCTGGGGGC CGCCATGGAG GAGGCCCTCA TCCTGGACAA CAAGTACACG 
GAGCACAGCA CCGTGGGCCT CGCCCAGCAG TGGGACCAGC TGGACCAGCT GGGCATGCGC 
ATGCAGCACA ACCTGGAGCA GCAGATCCAG GCCAGGAACA CAACAGGTGT GACTGAGGAG 
GCCCTCAAAG AATTCAGCAT GATGTTTAAA CACTTTGACA AGGACAAGTC TGGCAGGCTG 
AACCATCAGG AGTTCAAATC TTGCCTGCGC TCCCTGGGCT ATGACCTGCC CATGGTGGAG 
GAAGGGGAAC CTGACCCTGA GTTCGAGGCA ATCCTGGACA CGGTGGATCC GAACAGAGAT 
GGCCATGTCT CCTTGCAAGA ATACATGGCT TTCATGATCA GCCGCGAAAC TGAGAACGTC 
AAGTCCAGCG AGGAGATTGA GAGCGCCTTC CGGGCCCTCA GCTCAGAGGG AAAGCCTTAC 
GTGACCAAGG AGGAGCTCTA CCAGAACCTG ACCCGGGAAC AAGCCGACTA CTGCGTCTCC 
CACATGAAGC CCTACGTGGA CGGCAAGGGC CGCGAGCTCC CCACCGCGTT CGACTACGTG 
GAGTTCACCC GCTCGCTTTT CGTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_006717253.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_006717316.1 Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X11, mRNA.

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CloneID	OHu43461
Clone ID Related Accession (Same CDS sequence)	XM_006717253.1
Accession Version	XM_006717253.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACCG ATACCACCGC 
TTCAAGGAAC TCTCAACCCT TAGGCGTCAG AAGCTGGAAG ATTCCTATCG ATTCCAGTTC 
TTTCAAAGAG ATGCTGAAGA GCTGGAGAAA TGGATACAGG AAAAACTTCA GATTGCATCT 
GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGAAAGC TTCAGAAGCA TCAAGCATTT 
GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAGC TGGATGAAAC TGGAAACCTG 
ATGATCTCAG AAGGGCATTT TGCATCTGAA ACCATACGGA CCCGTTTGAT GGAGCTGCAC 
CGCCAGTGGG AATTACTTTT GGAGAAGATG CGAGAAAAAG GAATCAAACT GCTGCAGGCC 
CAGAAGTTGG TGCAGTACTT ACGAGAATGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCAATTG TTACTTCTGA AGAGCTGGGC CAGGATCTGG AGCATGTAGA GGTTTTACAG 
AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCTCATG AAGAAAGAGT TAATGAAGTG 
AACCAGTTTG CTGCCAAACT CATACAGGAG CAGCACCCTG AGGAGGAACT GATCAAGACT 
AAGCAGGATG AAGTCAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCTCT GCAGAGGCAG 
GGGAAGCTCT TTGGGGCAGC AGAAGTTCAG CGCTTTAACA GGGATGTGGA TGAGACTATC 
AGTTGGATTA AGGAAAAGGA GCAGTTAATG GCCTCTGATG ATTTTGGCCG AGACCTGGCA 
AGTGTTCAGG CTCTGCTTCG GAAGCACGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAA 
GACAAGGTCA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCCCA CCCTCTGAGT 
GCAACACAGA TTCAAGTGAA GCGAGAGGAA CTGATTACAA ACTGGGAGCA GATCCGCACC 
TTGGCGGCAG AGAGACATGC ACGGCTCAAT GATTCATACA GGCTTCAACG CTTCCTTGCT 
GACTTCCGTG ACCTCACCAG CTGGGTGACT GAGATGAAAG CCCTCATCAA TGCAGATGAG 
CTTGCCAGTG ATGTGGCTGG GGCTGAAGCC CTGCTAGATA GACACCAAGA GCACAAGGGT 
GAAATTGATG CCCATGAAGA CAGCTTCAAA TCTGCAGATG AATCTGGACA GGCACTGCTT 
GCTGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCGTCCT TTCCGAGGAG 
AGAGCGGCGC TGCTGGAGCT GTGGGAGCTG CGCAGGCAGC AGTACGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGGGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ATGAAGACTT GGGAGATTCC TTGGATAGTG TGGAAGCGCT TCTTAAGAAG 
CACGAAGACT TTGAGAAATC CCTTAGTGCC CAGGAGGAAA AGATTACAGC ATTAGATGAA 
TTTGCAACCA AGCTAATTCA GAACAACCAC TATGCAATGG AAGATGTGGC CACTCGCCGA 
GATGCTCTGT TGAGCCGCCG CAATGCCCTT CACGAGAGAG CCATGCGTCG CCGGGCCCAG 
CTAGCCGATT CTTTCCATCT GCAGCAGTTT TTCCGTGATT CTGATGAGCT CAAGAGTTGG 
GTCAATGAGA AGATGAAAAC TGCCACAGAT GAAGCTTATA AAGATCCATC CAACCTACAA 
GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CAGCAAACCA GAGCCGAATT 
GATGCCTTGG AGAAAGCTGG CCAAAAGCTG ATTGATGTCA ACCACTATGC CAAGGATGAA 
GTGGCAGCTC GTATGAATGA GGTGATCAGT TTGTGGAAGA AACTGCTAGA GGCCACTGAA 
CTGAAAGGAA TAAAGCTTCG TGAAGCCAAC CAGCAACAGC AATTTAATCG CAATGTTGAG 
GATATTGAAT TGTGGCTATA TGAAGTAGAA GGTCACTTGG CTTCGGATGA TTACGGCAAA 
GATCTTACCA ATGTGCAGAA CCTCCAGAAG AAACATGCAC TGCTAGAGGC AGATGTGGCT 
GCTCACCAGG ACCGAATTGA TGGCATCACC ATTCAGGCCC GCCAGTTCCA AGATGCTGGC 
CATTTTGATG CAGAAAACAT CAAGAAGAAA CAGGAAGCCC TCGTGGCTCG CTATGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCTCTGCG GTTGCAGCAG 
CTCTTCCGGG ATGTTGAGGA TGAGGAGACG TGGATTCGAG AGAAAGAGCC CATTGCCGCA 
TCTACCAACA GAGGTAAGGA TTTAATTGGG GTCCAGAATC TGCTAAAGAA ACATCAAGCC 
TTACAAGCAG AAATTGCTGG ACATGAACCA CGCATCAAAG CAGTTACACA GAAGGGGAAT 
GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAGCT TCACGAGCTG 
AACCAAAAGT GGGAGGCACT GAAAGCCAAA GCTTCCCAGC GTCGGCAGGA CCTGGAGGAC 
TCTCTGCAGG CCCAGCAGTA CTTTGCTGAT GCTAACGAGG CTGAATCCTG GATGCGGGAG 
AAGGAACCCA TTGTGGGCAG CACTGACTAT GGCAAGGACG AAGACTCTGC TGAGGCTCTA 
CTGAAGAAAC ACGAAGCTTT GATGTCAGAT CTCAGTGCCT ACGGCAGCAG CATCCAGGCT 
TTGCGAGAAC AAGCACAGTC CTGCCGGCAA CAAGTGGCCC CCACGGATGA TGAGACTGGG 
AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAGGGAG ATATCCTTAC CTTACTCAAC AGCACCAACA AGGATTGGTG GAAAGTGGAA 
GTGAACGATC GTCAGGGTTT TGTGCCGGCT GCGTACGTGA AGAAATTGGA CCCCGCCCAG 
TCAGCCTCCC GGGAGAATCT CCTGGAGGAG CAAGGCAGCA TAGCACTGCG GCAGGAGCAG 
ATTGACAATC AATATCATTC TCTGCTGGAA CTGGGTGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGTTGCA AGAAGTTTAT GTTGTTCCGT GAAGCGAATG AACTACAGCA ATGGATCAAT 
GAGAAGGAAG CCGCTCTGAC AAGTGAGGAG GTCGGAGCAG ACTTGGAGCA GGTTGAGGTG 
CTCCAGAAGA AGTTTGATGA CTTCCAGAAG GACCTGAAGG CCAATGAGTC ACGGTTGAAG 
GACATTAACA AGGTAGCTGA AGACCTGGAG TCTGAAGGTC TCATGGCAGA GGAGGTGCAG 
GCTGTGCAAC AACAGGAAGT GTATGGCATG ATGCCCAGGG ATGAAACTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGTCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTGA ATGAGCGCTG GCGGTCCCTA CAGCAGCTGG CCGAGGAACG GAGCCAGCTC 
TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CTGATGAAAC CAAAGAATGG 
ATTGAAGAGA AGAATCAAGC TCTAAACACA GACAATTATG GACATGATCT CGCCAGTGTC 
CAGGCCCTGC AACGCAAGCA TGAGGGCTTC GAGAGGGACC TTGCGGCTCT CGGTGACAAG 
GTAAACTCCC TTGGTGAAAC AGCAGAGCGC CTGATCCAGT CCCATCCCGA GTCAGCAGAA 
GACCTGCAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA GCAGCCTGGG GAAACGTGCA 
GATCAGCGCA AGGCAAAGTT GGGTGACTCC CACGACCTGC AGCGCTTCCT TAGCGATTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATA CGGGGGTTGG TGTCCTCAGA TGAGCTAGCC 
AAGGATGTCA CCGGAGCTGA GGCATTGCTG GAGCGACACC AGGAACACCG GACAGAAATC 
GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GACAGCAGCT GTTGGCTCAC 
GGACACTATG CCAGCCCTGA GATCAAGCAG AAACTTGATA TTCTTGACCA GGAGCGTGCA 
GACCTGGAGA AGGCCTGGGT TCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAACTGCAG 
CTGTTCCATC GGGACTGTGA GCAAGCTGAG AACTGGATGG CTGCCCGGGA GGCCTTCTTG 
AATACCGAAG ACAAAGGAGA CTCACTGGAC AGCGTAGAGG CTCTGATCAA AAAACATGAA 
GACTTTGACA AAGCGATTAA CGTCCAGGAA GAGAAGATTG CTGCTCTGCA GGCCTTTGCC 
GACCAGCTCA TCGCTGCCGG CCATTATGCC AAGGGAGACA TTTCTAGCCG GCGCAATGAG 
GTCTTGGACA GGTGGCGACG TCTGAAAGCC CAGATGATTG AGAAAAGGTC AAAGCTAGGA 
GAATCTCAAA CCCTCCAACA GTTCAGCCGG GATGTGGATG AGATTGAGGC TTGGATCAGT 
GAAAAATTGC AAACAGCGAG TGATGAGTCG TACAAGGATC CCACCAACAT CCAGAGCAAG 
CACCAGAAGC ACCAGGCTTT TGAAGCAGAG CTGCATGCCA ACGCTGACCG GATCCGTGGG 
GTTATCGACA TGGGCAACTC CCTCATTGAA CGTGGAGCCT GTGCCGGCAG TGAGGATGCT 
GTCAAGGCCC GCCTGGCTGC CTTAGCTGAC CAGTGGCAGT TCTTGGTGCA AAAGTCAGCG 
GAAAAGAGCC AGAAACTGAA AGAAGCCAAC AAGCAGCAGA ACTTCAACAC AGGGATCAAG 
GACTTTGACT TCTGGCTGTC TGAGGTGGAG GCCCTGCTGG CATCCGAAGA TTATGGCAAA 
GACCTAGCTT CTGTGAACAA CCTGCTGAAA AAGCATCAAC TGCTGGAAGC AGATATATCT 
GCCCATGAGG ATCGCCTGAA GGACCTGAAC AGCCAGGCAG ACAGCCTGAT GACCAGCAGT 
GCCTTCGACA CCTCCCAAGT AAAGGACAAG AGGGACACCA TCAACGGGCG CTTCCAGAAG 
ATCAAGAGCA TGGCGGCCTC CCGGCGAGCC AAGCTGAATG AATCCCATCG CCTGCACCAG 
TTCTTCCGGG ACATGGATGA CGAGGAGTCC TGGATCAAGG AGAAGAAGCT GCTGGTGGGC 
TCAGAGGACT ACGGCCGGGA CCTAACCGGC GTGCAGAACC TGAGGAAGAA GCACAAGCGG 
CTGGAAGCAG AACTGGCTGC GCATGAGCCG GCTATTCAGG GTGTCCTGGA CACTGGCAAG 
AAGCTGTCCG ATGACAACAC CATCGGGAAA GAGGAGATCC AGCAGCGGCT GGCGCAGTTT 
GTGGAGCACT GGAAAGAGCT GAAGCAGCTG GCAGCTGCCC GGGGTCAGCG GCTGGAAGAG 
TCCTTGGAAT ATCAGCAGTT TGTAGCCAAT GTGGAAGAGG AAGAAGCCTG GATCAATGAG 
AAAATGACCC TGGTGGCCAG CGAAGATTAT GGCGACACTC TTGCCGCCAT CCAGGGCTTA 
CTGAAGAAAC ATGAAGCTTT TGAGACAGAC TTCACCGTCC ACAAGGATCG CGTGAATGAT 
GTCTGCACCA ATGGACAAGA CCTCATTAAG AAGAACAATC ACCATGAGGA GAACATCTCT 
TCAAAGATGA AGGGCCTGAA CGGGAAAGTG TCAGACCTGG AGAAAGCTGC AGCCCAGAGA 
AAGGCGAAGC TGGATGAGAA CTCGGCCTTC CTTCAGTTCA ACTGGAAGGC GGACGTGGTG 
GAGTCCTGGA TCGGTGAAAA GGAGAACAGC TTGAAGACAG ATGATTATGG CCGAGACCTG 
TCTTCTGTGC AGACGCTCCT CACCAAACAG GAAACTTTTG ACGCTGGGCT GCAGGCCTTC 
CAGCAGGAAG GCATTGCCAA CATCACTGCC CTCAAAGATC AGCTTCTCGC CGCCAAACAC 
GTTCAGTCCA AGGCCATCGA GGCCCGGCAC GCCTCCCTCA TGAAGAGGTG GAGCCAGCTT 
CTGGCCAACT CAGCCGCCCG CAAGAAGAAG CTTCTGGAGG CTCAGAGTCA CTTCCGCAAG 
GTGGAGGACC TCTTCCTGAC CTTCGCCAAA AAGGCTTCTG CCTTCAACAG CTGGTTTGAA 
AATGCAGAGG AGGACTTAAC AGACCCCGTG CGCTGCAACT CCTTGGAAGA AATCAAAGCT 
TTGCGCGAGG CCCACGACGC CTTCCGCTCC TCCCTCAGCT CTGCCCAGGC TGACTTCAAC 
CAGCTGGCCG AGCTGGACCG CCAGATCAAG AGCTTCCGCG TAGCCTCCAA CCCCTACACC 
TGGTTTACCA TGGAGGCCCT GGAGGAGACC TGGAGGAACC TACAGAAAAT CATCAAGGAG 
AGGGAGCTGG AGCTGCAGAA GGAACAGCGG CGGCAGGAGG AGAACGACAA GCTGCGCCAG 
GAGTTTGCCC AGCACGCCAA CGCCTTCCAC CAGTGGATCC AAGAGACCAG GACATACCTC 
CTCGATGGGT CCTGTATGGT GGAAGAGTCG GGGACCCTCG AATCCCAGCT TGAAGCTACC 
AAACGCAAGC ACCAGGAAAT CCGAGCCATG AGAAGTCAGC TCAAAAAGAT CGAGGACCTG 
GGGGCCGCCA TGGAGGAGGC CCTCATCCTG GACAACAAGT ACACGGAGCA CAGCACCGTG 
GGCCTCGCCC AGCAGTGGGA CCAGCTGGAC CAGCTGGGCA TGCGCATGCA GCACAACCTG 
GAGCAGCAGA TCCAGGCCAG GAACACAACA GGTGTGACTG AGGAGGCCCT CAAAGAATTC 
AGCATGATGT TTAAACACTT TGACAAGGAC AAGTCTGGCA GGCTGAACCA TCAGGAGTTC 
AAATCTTGCC TGCGCTCCCT GGGCTATGAC CTGCCCATGG TGGAGGAAGG GGAACCTGAC 
CCTGAGTTCG AGGCAATCCT GGACACGGTG GATCCGAACA GAGATGGCCA TGTCTCCTTG 
CAAGAATACA TGGCTTTCAT GATCAGCCGC GAAACTGAGA ACGTCAAGTC CAGCGAGGAG 
ATTGAGAGCG CCTTCCGGGC CCTCAGCTCA GAGGGAAAGC CTTACGTGAC CAAGGAGGAG 
CTCTACCAGA ACCTGACCCG GGAACAAGCC GACTACTGCG TCTCCCACAT GAAGCCCTAC 
GTGGACGGCA AGGGCCGCGA GCTCCCCACC GCGTTCGACT ACGTGGAGTT CACCCGCTCG 
CTTTTCGTGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006717316.1
CDS	14..7408
Translation	MLSSFRKRRVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELASDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMRRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPTDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGMMPRDETDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKQKLDILDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAAGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINGRFQKIKSMAASRRAKLNESHRLHQFFRDMDDEESWIKEKKLLVGSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHASLMKRWSQLLANSAARKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_006717253.1
Organism	Homo sapiens(human)
Definition	Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006717253.1

ORF Insert Sequence:

ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACCG ATACCACCGC 
TTCAAGGAAC TCTCAACCCT TAGGCGTCAG AAGCTGGAAG ATTCCTATCG ATTCCAGTTC 
TTTCAAAGAG ATGCTGAAGA GCTGGAGAAA TGGATACAGG AAAAACTTCA GATTGCATCT 
GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGAAAGC TTCAGAAGCA TCAAGCATTT 
GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAGC TGGATGAAAC TGGAAACCTG 
ATGATCTCAG AAGGGCATTT TGCATCTGAA ACCATACGGA CCCGTTTGAT GGAGCTGCAC 
CGCCAGTGGG AATTACTTTT GGAGAAGATG CGAGAAAAAG GAATCAAACT GCTGCAGGCC 
CAGAAGTTGG TGCAGTACTT ACGAGAATGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCAATTG TTACTTCTGA AGAGCTGGGC CAGGATCTGG AGCATGTAGA GGTTTTACAG 
AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCTCATG AAGAAAGAGT TAATGAAGTG 
AACCAGTTTG CTGCCAAACT CATACAGGAG CAGCACCCTG AGGAGGAACT GATCAAGACT 
AAGCAGGATG AAGTCAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCTCT GCAGAGGCAG 
GGGAAGCTCT TTGGGGCAGC AGAAGTTCAG CGCTTTAACA GGGATGTGGA TGAGACTATC 
AGTTGGATTA AGGAAAAGGA GCAGTTAATG GCCTCTGATG ATTTTGGCCG AGACCTGGCA 
AGTGTTCAGG CTCTGCTTCG GAAGCACGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAA 
GACAAGGTCA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCCCA CCCTCTGAGT 
GCAACACAGA TTCAAGTGAA GCGAGAGGAA CTGATTACAA ACTGGGAGCA GATCCGCACC 
TTGGCGGCAG AGAGACATGC ACGGCTCAAT GATTCATACA GGCTTCAACG CTTCCTTGCT 
GACTTCCGTG ACCTCACCAG CTGGGTGACT GAGATGAAAG CCCTCATCAA TGCAGATGAG 
CTTGCCAGTG ATGTGGCTGG GGCTGAAGCC CTGCTAGATA GACACCAAGA GCACAAGGGT 
GAAATTGATG CCCATGAAGA CAGCTTCAAA TCTGCAGATG AATCTGGACA GGCACTGCTT 
GCTGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCGTCCT TTCCGAGGAG 
AGAGCGGCGC TGCTGGAGCT GTGGGAGCTG CGCAGGCAGC AGTACGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGGGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ATGAAGACTT GGGAGATTCC TTGGATAGTG TGGAAGCGCT TCTTAAGAAG 
CACGAAGACT TTGAGAAATC CCTTAGTGCC CAGGAGGAAA AGATTACAGC ATTAGATGAA 
TTTGCAACCA AGCTAATTCA GAACAACCAC TATGCAATGG AAGATGTGGC CACTCGCCGA 
GATGCTCTGT TGAGCCGCCG CAATGCCCTT CACGAGAGAG CCATGCGTCG CCGGGCCCAG 
CTAGCCGATT CTTTCCATCT GCAGCAGTTT TTCCGTGATT CTGATGAGCT CAAGAGTTGG 
GTCAATGAGA AGATGAAAAC TGCCACAGAT GAAGCTTATA AAGATCCATC CAACCTACAA 
GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CAGCAAACCA GAGCCGAATT 
GATGCCTTGG AGAAAGCTGG CCAAAAGCTG ATTGATGTCA ACCACTATGC CAAGGATGAA 
GTGGCAGCTC GTATGAATGA GGTGATCAGT TTGTGGAAGA AACTGCTAGA GGCCACTGAA 
CTGAAAGGAA TAAAGCTTCG TGAAGCCAAC CAGCAACAGC AATTTAATCG CAATGTTGAG 
GATATTGAAT TGTGGCTATA TGAAGTAGAA GGTCACTTGG CTTCGGATGA TTACGGCAAA 
GATCTTACCA ATGTGCAGAA CCTCCAGAAG AAACATGCAC TGCTAGAGGC AGATGTGGCT 
GCTCACCAGG ACCGAATTGA TGGCATCACC ATTCAGGCCC GCCAGTTCCA AGATGCTGGC 
CATTTTGATG CAGAAAACAT CAAGAAGAAA CAGGAAGCCC TCGTGGCTCG CTATGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCTCTGCG GTTGCAGCAG 
CTCTTCCGGG ATGTTGAGGA TGAGGAGACG TGGATTCGAG AGAAAGAGCC CATTGCCGCA 
TCTACCAACA GAGGTAAGGA TTTAATTGGG GTCCAGAATC TGCTAAAGAA ACATCAAGCC 
TTACAAGCAG AAATTGCTGG ACATGAACCA CGCATCAAAG CAGTTACACA GAAGGGGAAT 
GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAGCT TCACGAGCTG 
AACCAAAAGT GGGAGGCACT GAAAGCCAAA GCTTCCCAGC GTCGGCAGGA CCTGGAGGAC 
TCTCTGCAGG CCCAGCAGTA CTTTGCTGAT GCTAACGAGG CTGAATCCTG GATGCGGGAG 
AAGGAACCCA TTGTGGGCAG CACTGACTAT GGCAAGGACG AAGACTCTGC TGAGGCTCTA 
CTGAAGAAAC ACGAAGCTTT GATGTCAGAT CTCAGTGCCT ACGGCAGCAG CATCCAGGCT 
TTGCGAGAAC AAGCACAGTC CTGCCGGCAA CAAGTGGCCC CCACGGATGA TGAGACTGGG 
AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAGGGAG ATATCCTTAC CTTACTCAAC AGCACCAACA AGGATTGGTG GAAAGTGGAA 
GTGAACGATC GTCAGGGTTT TGTGCCGGCT GCGTACGTGA AGAAATTGGA CCCCGCCCAG 
TCAGCCTCCC GGGAGAATCT CCTGGAGGAG CAAGGCAGCA TAGCACTGCG GCAGGAGCAG 
ATTGACAATC AATATCATTC TCTGCTGGAA CTGGGTGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGTTGCA AGAAGTTTAT GTTGTTCCGT GAAGCGAATG AACTACAGCA ATGGATCAAT 
GAGAAGGAAG CCGCTCTGAC AAGTGAGGAG GTCGGAGCAG ACTTGGAGCA GGTTGAGGTG 
CTCCAGAAGA AGTTTGATGA CTTCCAGAAG GACCTGAAGG CCAATGAGTC ACGGTTGAAG 
GACATTAACA AGGTAGCTGA AGACCTGGAG TCTGAAGGTC TCATGGCAGA GGAGGTGCAG 
GCTGTGCAAC AACAGGAAGT GTATGGCATG ATGCCCAGGG ATGAAACTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGTCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTGA ATGAGCGCTG GCGGTCCCTA CAGCAGCTGG CCGAGGAACG GAGCCAGCTC 
TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CTGATGAAAC CAAAGAATGG 
ATTGAAGAGA AGAATCAAGC TCTAAACACA GACAATTATG GACATGATCT CGCCAGTGTC 
CAGGCCCTGC AACGCAAGCA TGAGGGCTTC GAGAGGGACC TTGCGGCTCT CGGTGACAAG 
GTAAACTCCC TTGGTGAAAC AGCAGAGCGC CTGATCCAGT CCCATCCCGA GTCAGCAGAA 
GACCTGCAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA GCAGCCTGGG GAAACGTGCA 
GATCAGCGCA AGGCAAAGTT GGGTGACTCC CACGACCTGC AGCGCTTCCT TAGCGATTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATA CGGGGGTTGG TGTCCTCAGA TGAGCTAGCC 
AAGGATGTCA CCGGAGCTGA GGCATTGCTG GAGCGACACC AGGAACACCG GACAGAAATC 
GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GACAGCAGCT GTTGGCTCAC 
GGACACTATG CCAGCCCTGA GATCAAGCAG AAACTTGATA TTCTTGACCA GGAGCGTGCA 
GACCTGGAGA AGGCCTGGGT TCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAACTGCAG 
CTGTTCCATC GGGACTGTGA GCAAGCTGAG AACTGGATGG CTGCCCGGGA GGCCTTCTTG 
AATACCGAAG ACAAAGGAGA CTCACTGGAC AGCGTAGAGG CTCTGATCAA AAAACATGAA 
GACTTTGACA AAGCGATTAA CGTCCAGGAA GAGAAGATTG CTGCTCTGCA GGCCTTTGCC 
GACCAGCTCA TCGCTGCCGG CCATTATGCC AAGGGAGACA TTTCTAGCCG GCGCAATGAG 
GTCTTGGACA GGTGGCGACG TCTGAAAGCC CAGATGATTG AGAAAAGGTC AAAGCTAGGA 
GAATCTCAAA CCCTCCAACA GTTCAGCCGG GATGTGGATG AGATTGAGGC TTGGATCAGT 
GAAAAATTGC AAACAGCGAG TGATGAGTCG TACAAGGATC CCACCAACAT CCAGAGCAAG 
CACCAGAAGC ACCAGGCTTT TGAAGCAGAG CTGCATGCCA ACGCTGACCG GATCCGTGGG 
GTTATCGACA TGGGCAACTC CCTCATTGAA CGTGGAGCCT GTGCCGGCAG TGAGGATGCT 
GTCAAGGCCC GCCTGGCTGC CTTAGCTGAC CAGTGGCAGT TCTTGGTGCA AAAGTCAGCG 
GAAAAGAGCC AGAAACTGAA AGAAGCCAAC AAGCAGCAGA ACTTCAACAC AGGGATCAAG 
GACTTTGACT TCTGGCTGTC TGAGGTGGAG GCCCTGCTGG CATCCGAAGA TTATGGCAAA 
GACCTAGCTT CTGTGAACAA CCTGCTGAAA AAGCATCAAC TGCTGGAAGC AGATATATCT 
GCCCATGAGG ATCGCCTGAA GGACCTGAAC AGCCAGGCAG ACAGCCTGAT GACCAGCAGT 
GCCTTCGACA CCTCCCAAGT AAAGGACAAG AGGGACACCA TCAACGGGCG CTTCCAGAAG 
ATCAAGAGCA TGGCGGCCTC CCGGCGAGCC AAGCTGAATG AATCCCATCG CCTGCACCAG 
TTCTTCCGGG ACATGGATGA CGAGGAGTCC TGGATCAAGG AGAAGAAGCT GCTGGTGGGC 
TCAGAGGACT ACGGCCGGGA CCTAACCGGC GTGCAGAACC TGAGGAAGAA GCACAAGCGG 
CTGGAAGCAG AACTGGCTGC GCATGAGCCG GCTATTCAGG GTGTCCTGGA CACTGGCAAG 
AAGCTGTCCG ATGACAACAC CATCGGGAAA GAGGAGATCC AGCAGCGGCT GGCGCAGTTT 
GTGGAGCACT GGAAAGAGCT GAAGCAGCTG GCAGCTGCCC GGGGTCAGCG GCTGGAAGAG 
TCCTTGGAAT ATCAGCAGTT TGTAGCCAAT GTGGAAGAGG AAGAAGCCTG GATCAATGAG 
AAAATGACCC TGGTGGCCAG CGAAGATTAT GGCGACACTC TTGCCGCCAT CCAGGGCTTA 
CTGAAGAAAC ATGAAGCTTT TGAGACAGAC TTCACCGTCC ACAAGGATCG CGTGAATGAT 
GTCTGCACCA ATGGACAAGA CCTCATTAAG AAGAACAATC ACCATGAGGA GAACATCTCT 
TCAAAGATGA AGGGCCTGAA CGGGAAAGTG TCAGACCTGG AGAAAGCTGC AGCCCAGAGA 
AAGGCGAAGC TGGATGAGAA CTCGGCCTTC CTTCAGTTCA ACTGGAAGGC GGACGTGGTG 
GAGTCCTGGA TCGGTGAAAA GGAGAACAGC TTGAAGACAG ATGATTATGG CCGAGACCTG 
TCTTCTGTGC AGACGCTCCT CACCAAACAG GAAACTTTTG ACGCTGGGCT GCAGGCCTTC 
CAGCAGGAAG GCATTGCCAA CATCACTGCC CTCAAAGATC AGCTTCTCGC CGCCAAACAC 
GTTCAGTCCA AGGCCATCGA GGCCCGGCAC GCCTCCCTCA TGAAGAGGTG GAGCCAGCTT 
CTGGCCAACT CAGCCGCCCG CAAGAAGAAG CTTCTGGAGG CTCAGAGTCA CTTCCGCAAG 
GTGGAGGACC TCTTCCTGAC CTTCGCCAAA AAGGCTTCTG CCTTCAACAG CTGGTTTGAA 
AATGCAGAGG AGGACTTAAC AGACCCCGTG CGCTGCAACT CCTTGGAAGA AATCAAAGCT 
TTGCGCGAGG CCCACGACGC CTTCCGCTCC TCCCTCAGCT CTGCCCAGGC TGACTTCAAC 
CAGCTGGCCG AGCTGGACCG CCAGATCAAG AGCTTCCGCG TAGCCTCCAA CCCCTACACC 
TGGTTTACCA TGGAGGCCCT GGAGGAGACC TGGAGGAACC TACAGAAAAT CATCAAGGAG 
AGGGAGCTGG AGCTGCAGAA GGAACAGCGG CGGCAGGAGG AGAACGACAA GCTGCGCCAG 
GAGTTTGCCC AGCACGCCAA CGCCTTCCAC CAGTGGATCC AAGAGACCAG GACATACCTC 
CTCGATGGGT CCTGTATGGT GGAAGAGTCG GGGACCCTCG AATCCCAGCT TGAAGCTACC 
AAACGCAAGC ACCAGGAAAT CCGAGCCATG AGAAGTCAGC TCAAAAAGAT CGAGGACCTG 
GGGGCCGCCA TGGAGGAGGC CCTCATCCTG GACAACAAGT ACACGGAGCA CAGCACCGTG 
GGCCTCGCCC AGCAGTGGGA CCAGCTGGAC CAGCTGGGCA TGCGCATGCA GCACAACCTG 
GAGCAGCAGA TCCAGGCCAG GAACACAACA GGTGTGACTG AGGAGGCCCT CAAAGAATTC 
AGCATGATGT TTAAACACTT TGACAAGGAC AAGTCTGGCA GGCTGAACCA TCAGGAGTTC 
AAATCTTGCC TGCGCTCCCT GGGCTATGAC CTGCCCATGG TGGAGGAAGG GGAACCTGAC 
CCTGAGTTCG AGGCAATCCT GGACACGGTG GATCCGAACA GAGATGGCCA TGTCTCCTTG 
CAAGAATACA TGGCTTTCAT GATCAGCCGC GAAACTGAGA ACGTCAAGTC CAGCGAGGAG 
ATTGAGAGCG CCTTCCGGGC CCTCAGCTCA GAGGGAAAGC CTTACGTGAC CAAGGAGGAG 
CTCTACCAGA ACCTGACCCG GGAACAAGCC GACTACTGCG TCTCCCACAT GAAGCCCTAC 
GTGGACGGCA AGGGCCGCGA GCTCCCCACC GCGTTCGACT ACGTGGAGTT CACCCGCTCG 
CTTTTCGTGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_006717254.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006717317.1 Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X3, mRNA.

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CloneID	OHu43462
Clone ID Related Accession (Same CDS sequence)	NM_001363759.2 , XM_006717254.2 , NM_001363759.1
Accession Version	XM_006717254.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7497bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006717254.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAA GTGGGGTCAA AGTGCTGGAA ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTAG ACCGATACCA CCGCTTCAAG GAACTCTCAA CCCTTAGGCG TCAGAAGCTG 
GAAGATTCCT ATCGATTCCA GTTCTTTCAA AGAGATGCTG AAGAGCTGGA GAAATGGATA 
CAGGAAAAAC TTCAGATTGC ATCTGATGAG AATTATAAAG ACCCAACCAA CTTGCAGGGA 
AAGCTTCAGA AGCATCAAGC ATTTGAAGCT GAAGTGCAGG CCAACTCAGG AGCCATTGTT 
AAGCTGGATG AAACTGGAAA CCTGATGATC TCAGAAGGGC ATTTTGCATC TGAAACCATA 
CGGACCCGTT TGATGGAGCT GCACCGCCAG TGGGAATTAC TTTTGGAGAA GATGCGAGAA 
AAAGGAATCA AACTGCTGCA GGCCCAGAAG TTGGTGCAGT ACTTACGAGA ATGTGAGGAC 
GTGATGGACT GGATCAATGA CAAGGAAGCA ATTGTTACTT CTGAAGAGCT GGGCCAGGAT 
CTGGAGCATG TAGAGGTTTT ACAGAAGAAA TTTGAAGAGT TTCAAACAGA TATGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTGAACCAG TTTGCTGCCA AACTCATACA GGAGCAGCAC 
CCTGAGGAGG AACTGATCAA GACTAAGCAG GATGAAGTCA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CTCTGCAGAG GCAGGGGAAG CTCTTTGGGG CAGCAGAAGT TCAGCGCTTT 
AACAGGGATG TGGATGAGAC TATCAGTTGG ATTAAGGAAA AGGAGCAGTT AATGGCCTCT 
GATGATTTTG GCCGAGACCT GGCAAGTGTT CAGGCTCTGC TTCGGAAGCA CGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAAGACAAG GTCAAAGCCC TGTGTGCTGA GGCTGACCGC 
CTGCAACAGT CCCACCCTCT GAGTGCAACA CAGATTCAAG TGAAGCGAGA GGAACTGATT 
ACAAACTGGG AGCAGATCCG CACCTTGGCG GCAGAGAGAC ATGCACGGCT CAATGATTCA 
TACAGGCTTC AACGCTTCCT TGCTGACTTC CGTGACCTCA CCAGCTGGGT GACTGAGATG 
AAAGCCCTCA TCAATGCAGA TGAGCTTGCC AGTGATGTGG CTGGGGCTGA AGCCCTGCTA 
GATAGACACC AAGAGCACAA GGGTGAAATT GATGCCCATG AAGACAGCTT CAAATCTGCA 
GATGAATCTG GACAGGCACT GCTTGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGACCG TCCTTTCCGA GGAGAGAGCG GCGCTGCTGG AGCTGTGGGA GCTGCGCAGG 
CAGCAGTACG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GGGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAATGAAG ACTTGGGAGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTTAA GAAGCACGAA GACTTTGAGA AATCCCTTAG TGCCCAGGAG 
GAAAAGATTA CAGCATTAGA TGAATTTGCA ACCAAGCTAA TTCAGAACAA CCACTATGCA 
ATGGAAGATG TGGCCACTCG CCGAGATGCT CTGTTGAGCC GCCGCAATGC CCTTCACGAG 
AGAGCCATGC GTCGCCGGGC CCAGCTAGCC GATTCTTTCC ATCTGCAGCA GTTTTTCCGT 
GATTCTGATG AGCTCAAGAG TTGGGTCAAT GAGAAGATGA AAACTGCCAC AGATGAAGCT 
TATAAAGATC CATCCAACCT ACAAGGAAAA GTACAGAAGC ATCAGGCTTT TGAGGCTGAG 
CTCTCAGCAA ACCAGAGCCG AATTGATGCC TTGGAGAAAG CTGGCCAAAA GCTGATTGAT 
GTCAACCACT ATGCCAAGGA TGAAGTGGCA GCTCGTATGA ATGAGGTGAT CAGTTTGTGG 
AAGAAACTGC TAGAGGCCAC TGAACTGAAA GGAATAAAGC TTCGTGAAGC CAACCAGCAA 
CAGCAATTTA ATCGCAATGT TGAGGATATT GAATTGTGGC TATATGAAGT AGAAGGTCAC 
TTGGCTTCGG ATGATTACGG CAAAGATCTT ACCAATGTGC AGAACCTCCA GAAGAAACAT 
GCACTGCTAG AGGCAGATGT GGCTGCTCAC CAGGACCGAA TTGATGGCAT CACCATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTT GATGCAGAAA ACATCAAGAA GAAACAGGAA 
GCCCTCGTGG CTCGCTATGA GGCACTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCCGATTCTC TGCGGTTGCA GCAGCTCTTC CGGGATGTTG AGGATGAGGA GACGTGGATT 
CGAGAGAAAG AGCCCATTGC CGCATCTACC AACAGAGGTA AGGATTTAAT TGGGGTCCAG 
AATCTGCTAA AGAAACATCA AGCCTTACAA GCAGAAATTG CTGGACATGA ACCACGCATC 
AAAGCAGTTA CACAGAAGGG GAATGCCATG GTGGAGGAAG GCCATTTTGC TGCAGAGGAT 
GTGAAGGCCA AGCTTCACGA GCTGAACCAA AAGTGGGAGG CACTGAAAGC CAAAGCTTCC 
CAGCGTCGGC AGGACCTGGA GGACTCTCTG CAGGCCCAGC AGTACTTTGC TGATGCTAAC 
GAGGCTGAAT CCTGGATGCG GGAGAAGGAA CCCATTGTGG GCAGCACTGA CTATGGCAAG 
GACGAAGACT CTGCTGAGGC TCTACTGAAG AAACACGAAG CTTTGATGTC AGATCTCAGT 
GCCTACGGCA GCAGCATCCA GGCTTTGCGA GAACAAGCAC AGTCCTGCCG GCAACAAGTG 
GCCCCCACGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CTCTCTACGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAG GGAGATATCC TTACCTTACT CAACAGCACC 
AACAAGGATT GGTGGAAAGT GGAAGTGAAC GATCGTCAGG GTTTTGTGCC GGCTGCGTAC 
GTGAAGAAAT TGGACCCCGC CCAGTCAGCC TCCCGGGAGA ATCTCCTGGA GGAGCAAGGC 
AGCATAGCAC TGCGGCAGGA GCAGATTGAC AATCAGACAC GCATAACTAA GGAGGCCGGC 
AGTGTATCTC TGCGTATGAA GCAGGTGGAA GAACTATATC ATTCTCTGCT GGAACTGGGT 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TTATGTTGTT CCGTGAAGCG 
AATGAACTAC AGCAATGGAT CAATGAGAAG GAAGCCGCTC TGACAAGTGA GGAGGTCGGA 
GCAGACTTGG AGCAGGTTGA GGTGCTCCAG AAGAAGTTTG ATGACTTCCA GAAGGACCTG 
AAGGCCAATG AGTCACGGTT GAAGGACATT AACAAGGTAG CTGAAGACCT GGAGTCTGAA 
GGTCTCATGG CAGAGGAGGT GCAGGCTGTG CAACAACAGG AAGTGTATGG CATGATGCCC 
AGGGATGAAA CTGATTCCAA GACAGCCTCC CCGTGGAAGT CTGCTCGTCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTACAGCAG 
CTGGCCGAGG AACGGAGCCA GCTCTTGGGC AGCGCCCATG AAGTACAGAG GTTCCACAGA 
GATGCTGATG AAACCAAAGA ATGGATTGAA GAGAAGAATC AAGCTCTAAA CACAGACAAT 
TATGGACATG ATCTCGCCAG TGTCCAGGCC CTGCAACGCA AGCATGAGGG CTTCGAGAGG 
GACCTTGCGG CTCTCGGTGA CAAGGTAAAC TCCCTTGGTG AAACAGCAGA GCGCCTGATC 
CAGTCCCATC CCGAGTCAGC AGAAGACCTG CAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGAGCAGCC TGGGGAAACG TGCAGATCAG CGCAAGGCAA AGTTGGGTGA CTCCCACGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGGGGG 
TTGGTGTCCT CAGATGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCATT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATCGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGACAGC AGCTGTTGGC TCACGGACAC TATGCCAGCC CTGAGATCAA GCAGAAACTT 
GATATTCTTG ACCAGGAGCG TGCAGACCTG GAGAAGGCCT GGGTTCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAACT GCAGCTGTTC CATCGGGACT GTGAGCAAGC TGAGAACTGG 
ATGGCTGCCC GGGAGGCCTT CTTGAATACC GAAGACAAAG GAGACTCACT GGACAGCGTA 
GAGGCTCTGA TCAAAAAACA TGAAGACTTT GACAAAGCGA TTAACGTCCA GGAAGAGAAG 
ATTGCTGCTC TGCAGGCCTT TGCCGACCAG CTCATCGCTG CCGGCCATTA TGCCAAGGGA 
GACATTTCTA GCCGGCGCAA TGAGGTCTTG GACAGGTGGC GACGTCTGAA AGCCCAGATG 
ATTGAGAAAA GGTCAAAGCT AGGAGAATCT CAAACCCTCC AACAGTTCAG CCGGGATGTG 
GATGAGATTG AGGCTTGGAT CAGTGAAAAA TTGCAAACAG CGAGTGATGA GTCGTACAAG 
GATCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ACCAGAAGCA CCAGGCTTTT 
GAAGCAGAGC TGCATGCCAA CGCTGACCGG ATCCGTGGGG TTATCGACAT GGGCAACTCC 
CTCATTGAAC GTGGAGCCTG TGCCGGCAGT GAGGATGCTG TCAAGGCCCG CCTGGCTGCC 
TTAGCTGACC AGTGGCAGTT CTTGGTGCAA AAGTCAGCGG AAAAGAGCCA GAAACTGAAA 
GAAGCCAACA AGCAGCAGAA CTTCAACACA GGGATCAAGG ACTTTGACTT CTGGCTGTCT 
GAGGTGGAGG CCCTGCTGGC ATCCGAAGAT TATGGCAAAG ACCTAGCTTC TGTGAACAAC 
CTGCTGAAAA AGCATCAACT GCTGGAAGCA GATATATCTG CCCATGAGGA TCGCCTGAAG 
GACCTGAACA GCCAGGCAGA CAGCCTGATG ACCAGCAGTG CCTTCGACAC CTCCCAAGTA 
AAGGACAAGA GGGACACCAT CAACGGGCGC TTCCAGAAGA TCAAGAGCAT GGCGGCCTCC 
CGGCGAGCCA AGCTGAATGA ATCCCATCGC CTGCACCAGT TCTTCCGGGA CATGGATGAC 
GAGGAGTCCT GGATCAAGGA GAAGAAGCTG CTGGTGGGCT CAGAGGACTA CGGCCGGGAC 
CTAACCGGCG TGCAGAACCT GAGGAAGAAG CACAAGCGGC TGGAAGCAGA ACTGGCTGCG 
CATGAGCCGG CTATTCAGGG TGTCCTGGAC ACTGGCAAGA AGCTGTCCGA TGACAACACC 
ATCGGGAAAG AGGAGATCCA GCAGCGGCTG GCGCAGTTTG TGGAGCACTG GAAAGAGCTG 
AAGCAGCTGG CAGCTGCCCG GGGTCAGCGG CTGGAAGAGT CCTTGGAATA TCAGCAGTTT 
GTAGCCAATG TGGAAGAGGA AGAAGCCTGG ATCAATGAGA AAATGACCCT GGTGGCCAGC 
GAAGATTATG GCGACACTCT TGCCGCCATC CAGGGCTTAC TGAAGAAACA TGAAGCTTTT 
GAGACAGACT TCACCGTCCA CAAGGATCGC GTGAATGATG TCTGCACCAA TGGACAAGAC 
CTCATTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CAAAGATGAA GGGCCTGAAC 
GGGAAAGTGT CAGACCTGGA GAAAGCTGCA GCCCAGAGAA AGGCGAAGCT GGATGAGAAC 
TCGGCCTTCC TTCAGTTCAA CTGGAAGGCG GACGTGGTGG AGTCCTGGAT CGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGC CGAGACCTGT CTTCTGTGCA GACGCTCCTC 
ACCAAACAGG AAACTTTTGA CGCTGGGCTG CAGGCCTTCC AGCAGGAAGG CATTGCCAAC 
ATCACTGCCC TCAAAGATCA GCTTCTCGCC GCCAAACACG TTCAGTCCAA GGCCATCGAG 
GCCCGGCACG CCTCCCTCAT GAAGAGGTGG AGCCAGCTTC TGGCCAACTC AGCCGCCCGC 
AAGAAGAAGC TTCTGGAGGC TCAGAGTCAC TTCCGCAAGG TGGAGGACCT CTTCCTGACC 
TTCGCCAAAA AGGCTTCTGC CTTCAACAGC TGGTTTGAAA ATGCAGAGGA GGACTTAACA 
GACCCCGTGC GCTGCAACTC CTTGGAAGAA ATCAAAGCTT TGCGCGAGGC CCACGACGCC 
TTCCGCTCCT CCCTCAGCTC TGCCCAGGCT GACTTCAACC AGCTGGCCGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT AGCCTCCAAC CCCTACACCT GGTTTACCAT GGAGGCCCTG 
GAGGAGACCT GGAGGAACCT ACAGAAAATC ATCAAGGAGA GGGAGCTGGA GCTGCAGAAG 
GAACAGCGGC GGCAGGAGGA GAACGACAAG CTGCGCCAGG AGTTTGCCCA GCACGCCAAC 
GCCTTCCACC AGTGGATCCA AGAGACCAGG ACATACCTCC TCGATGGCAT AGCATATCGT 
CGGGTCATTC GTGTCTATCA GTATGAAGTT GGGGATGATC TGTCTGGAAG GTCCTGTATG 
GTGGAAGAGT CGGGGACCCT CGAATCCCAG CTTGAAGCTA CCAAACGCAA GCACCAGGAA 
ATCCGAGCCA TGAGAAGTCA GCTCAAAAAG ATCGAGGACC TGGGGGCCGC CATGGAGGAG 
GCCCTCATCC TGGACAACAA GTACACGGAG CACAGCACCG TGGGCCTCGC CCAGCAGTGG 
GACCAGCTGG ACCAGCTGGG CATGCGCATG CAGCACAACC TGGAGCAGCA GATCCAGGCC 
AGGAACACAA CAGGTGTGAC TGAGGAGGCC CTCAAAGAAT TCAGCATGAT GTTTAAACAC 
TTTGACAAGG ACAAGTCTGG CAGGCTGAAC CATCAGGAGT TCAAATCTTG CCTGCGCTCC 
CTGGGCTATG ACCTGCCCAT GGTGGAGGAA GGGGAACCTG ACCCTGAGTT CGAGGCAATC 
CTGGACACGG TGGATCCGAA CAGAGATGGC CATGTCTCCT TGCAAGAATA CATGGCTTTC 
ATGATCAGCC GCGAAACTGA GAACGTCAAG TCCAGCGAGG AGATTGAGAG CGCCTTCCGG 
GCCCTCAGCT CAGAGGGAAA GCCTTACGTG ACCAAGGAGG AGCTCTACCA GAACCTGACC 
CGGGAACAAG CCGACTACTG CGTCTCCCAC ATGAAGCCCT ACGTGGACGG CAAGGGCCGC 
GAGCTCCCCA CCGCGTTCGA CTACGTGGAG TTCACCCGCT CGCTTTTCGT GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006717317.1
CDS	137..7633
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELASDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMRRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPTDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRITKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGMMPRDETDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKQKLDILDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAAGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINGRFQKIKSMAASRRAKLNESHRLHQFFRDMDDEESWIKEKKLLVGSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHASLMKRWSQLLANSAARKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGIAYRRVIRVYQYEVGDDLSGRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_006717254.2
Organism	Homo sapiens(human)
Definition	Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006717254.2

ORF Insert Sequence:

ATGGACCCAA GTGGGGTCAA AGTGCTGGAA ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTAG ACCGATACCA CCGCTTCAAG GAACTCTCAA CCCTTAGGCG TCAGAAGCTG 
GAAGATTCCT ATCGATTCCA GTTCTTTCAA AGAGATGCTG AAGAGCTGGA GAAATGGATA 
CAGGAAAAAC TTCAGATTGC ATCTGATGAG AATTATAAAG ACCCAACCAA CTTGCAGGGA 
AAGCTTCAGA AGCATCAAGC ATTTGAAGCT GAAGTGCAGG CCAACTCAGG AGCCATTGTT 
AAGCTGGATG AAACTGGAAA CCTGATGATC TCAGAAGGGC ATTTTGCATC TGAAACCATA 
CGGACCCGTT TGATGGAGCT GCACCGCCAG TGGGAATTAC TTTTGGAGAA GATGCGAGAA 
AAAGGAATCA AACTGCTGCA GGCCCAGAAG TTGGTGCAGT ACTTACGAGA ATGTGAGGAC 
GTGATGGACT GGATCAATGA CAAGGAAGCA ATTGTTACTT CTGAAGAGCT GGGCCAGGAT 
CTGGAGCATG TAGAGGTTTT ACAGAAGAAA TTTGAAGAGT TTCAAACAGA TATGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTGAACCAG TTTGCTGCCA AACTCATACA GGAGCAGCAC 
CCTGAGGAGG AACTGATCAA GACTAAGCAG GATGAAGTCA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CTCTGCAGAG GCAGGGGAAG CTCTTTGGGG CAGCAGAAGT TCAGCGCTTT 
AACAGGGATG TGGATGAGAC TATCAGTTGG ATTAAGGAAA AGGAGCAGTT AATGGCCTCT 
GATGATTTTG GCCGAGACCT GGCAAGTGTT CAGGCTCTGC TTCGGAAGCA CGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAAGACAAG GTCAAAGCCC TGTGTGCTGA GGCTGACCGC 
CTGCAACAGT CCCACCCTCT GAGTGCAACA CAGATTCAAG TGAAGCGAGA GGAACTGATT 
ACAAACTGGG AGCAGATCCG CACCTTGGCG GCAGAGAGAC ATGCACGGCT CAATGATTCA 
TACAGGCTTC AACGCTTCCT TGCTGACTTC CGTGACCTCA CCAGCTGGGT GACTGAGATG 
AAAGCCCTCA TCAATGCAGA TGAGCTTGCC AGTGATGTGG CTGGGGCTGA AGCCCTGCTA 
GATAGACACC AAGAGCACAA GGGTGAAATT GATGCCCATG AAGACAGCTT CAAATCTGCA 
GATGAATCTG GACAGGCACT GCTTGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGACCG TCCTTTCCGA GGAGAGAGCG GCGCTGCTGG AGCTGTGGGA GCTGCGCAGG 
CAGCAGTACG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GGGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAATGAAG ACTTGGGAGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTTAA GAAGCACGAA GACTTTGAGA AATCCCTTAG TGCCCAGGAG 
GAAAAGATTA CAGCATTAGA TGAATTTGCA ACCAAGCTAA TTCAGAACAA CCACTATGCA 
ATGGAAGATG TGGCCACTCG CCGAGATGCT CTGTTGAGCC GCCGCAATGC CCTTCACGAG 
AGAGCCATGC GTCGCCGGGC CCAGCTAGCC GATTCTTTCC ATCTGCAGCA GTTTTTCCGT 
GATTCTGATG AGCTCAAGAG TTGGGTCAAT GAGAAGATGA AAACTGCCAC AGATGAAGCT 
TATAAAGATC CATCCAACCT ACAAGGAAAA GTACAGAAGC ATCAGGCTTT TGAGGCTGAG 
CTCTCAGCAA ACCAGAGCCG AATTGATGCC TTGGAGAAAG CTGGCCAAAA GCTGATTGAT 
GTCAACCACT ATGCCAAGGA TGAAGTGGCA GCTCGTATGA ATGAGGTGAT CAGTTTGTGG 
AAGAAACTGC TAGAGGCCAC TGAACTGAAA GGAATAAAGC TTCGTGAAGC CAACCAGCAA 
CAGCAATTTA ATCGCAATGT TGAGGATATT GAATTGTGGC TATATGAAGT AGAAGGTCAC 
TTGGCTTCGG ATGATTACGG CAAAGATCTT ACCAATGTGC AGAACCTCCA GAAGAAACAT 
GCACTGCTAG AGGCAGATGT GGCTGCTCAC CAGGACCGAA TTGATGGCAT CACCATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTT GATGCAGAAA ACATCAAGAA GAAACAGGAA 
GCCCTCGTGG CTCGCTATGA GGCACTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCCGATTCTC TGCGGTTGCA GCAGCTCTTC CGGGATGTTG AGGATGAGGA GACGTGGATT 
CGAGAGAAAG AGCCCATTGC CGCATCTACC AACAGAGGTA AGGATTTAAT TGGGGTCCAG 
AATCTGCTAA AGAAACATCA AGCCTTACAA GCAGAAATTG CTGGACATGA ACCACGCATC 
AAAGCAGTTA CACAGAAGGG GAATGCCATG GTGGAGGAAG GCCATTTTGC TGCAGAGGAT 
GTGAAGGCCA AGCTTCACGA GCTGAACCAA AAGTGGGAGG CACTGAAAGC CAAAGCTTCC 
CAGCGTCGGC AGGACCTGGA GGACTCTCTG CAGGCCCAGC AGTACTTTGC TGATGCTAAC 
GAGGCTGAAT CCTGGATGCG GGAGAAGGAA CCCATTGTGG GCAGCACTGA CTATGGCAAG 
GACGAAGACT CTGCTGAGGC TCTACTGAAG AAACACGAAG CTTTGATGTC AGATCTCAGT 
GCCTACGGCA GCAGCATCCA GGCTTTGCGA GAACAAGCAC AGTCCTGCCG GCAACAAGTG 
GCCCCCACGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CTCTCTACGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAG GGAGATATCC TTACCTTACT CAACAGCACC 
AACAAGGATT GGTGGAAAGT GGAAGTGAAC GATCGTCAGG GTTTTGTGCC GGCTGCGTAC 
GTGAAGAAAT TGGACCCCGC CCAGTCAGCC TCCCGGGAGA ATCTCCTGGA GGAGCAAGGC 
AGCATAGCAC TGCGGCAGGA GCAGATTGAC AATCAGACAC GCATAACTAA GGAGGCCGGC 
AGTGTATCTC TGCGTATGAA GCAGGTGGAA GAACTATATC ATTCTCTGCT GGAACTGGGT 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TTATGTTGTT CCGTGAAGCG 
AATGAACTAC AGCAATGGAT CAATGAGAAG GAAGCCGCTC TGACAAGTGA GGAGGTCGGA 
GCAGACTTGG AGCAGGTTGA GGTGCTCCAG AAGAAGTTTG ATGACTTCCA GAAGGACCTG 
AAGGCCAATG AGTCACGGTT GAAGGACATT AACAAGGTAG CTGAAGACCT GGAGTCTGAA 
GGTCTCATGG CAGAGGAGGT GCAGGCTGTG CAACAACAGG AAGTGTATGG CATGATGCCC 
AGGGATGAAA CTGATTCCAA GACAGCCTCC CCGTGGAAGT CTGCTCGTCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTACAGCAG 
CTGGCCGAGG AACGGAGCCA GCTCTTGGGC AGCGCCCATG AAGTACAGAG GTTCCACAGA 
GATGCTGATG AAACCAAAGA ATGGATTGAA GAGAAGAATC AAGCTCTAAA CACAGACAAT 
TATGGACATG ATCTCGCCAG TGTCCAGGCC CTGCAACGCA AGCATGAGGG CTTCGAGAGG 
GACCTTGCGG CTCTCGGTGA CAAGGTAAAC TCCCTTGGTG AAACAGCAGA GCGCCTGATC 
CAGTCCCATC CCGAGTCAGC AGAAGACCTG CAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGAGCAGCC TGGGGAAACG TGCAGATCAG CGCAAGGCAA AGTTGGGTGA CTCCCACGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGGGGG 
TTGGTGTCCT CAGATGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCATT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATCGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGACAGC AGCTGTTGGC TCACGGACAC TATGCCAGCC CTGAGATCAA GCAGAAACTT 
GATATTCTTG ACCAGGAGCG TGCAGACCTG GAGAAGGCCT GGGTTCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAACT GCAGCTGTTC CATCGGGACT GTGAGCAAGC TGAGAACTGG 
ATGGCTGCCC GGGAGGCCTT CTTGAATACC GAAGACAAAG GAGACTCACT GGACAGCGTA 
GAGGCTCTGA TCAAAAAACA TGAAGACTTT GACAAAGCGA TTAACGTCCA GGAAGAGAAG 
ATTGCTGCTC TGCAGGCCTT TGCCGACCAG CTCATCGCTG CCGGCCATTA TGCCAAGGGA 
GACATTTCTA GCCGGCGCAA TGAGGTCTTG GACAGGTGGC GACGTCTGAA AGCCCAGATG 
ATTGAGAAAA GGTCAAAGCT AGGAGAATCT CAAACCCTCC AACAGTTCAG CCGGGATGTG 
GATGAGATTG AGGCTTGGAT CAGTGAAAAA TTGCAAACAG CGAGTGATGA GTCGTACAAG 
GATCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ACCAGAAGCA CCAGGCTTTT 
GAAGCAGAGC TGCATGCCAA CGCTGACCGG ATCCGTGGGG TTATCGACAT GGGCAACTCC 
CTCATTGAAC GTGGAGCCTG TGCCGGCAGT GAGGATGCTG TCAAGGCCCG CCTGGCTGCC 
TTAGCTGACC AGTGGCAGTT CTTGGTGCAA AAGTCAGCGG AAAAGAGCCA GAAACTGAAA 
GAAGCCAACA AGCAGCAGAA CTTCAACACA GGGATCAAGG ACTTTGACTT CTGGCTGTCT 
GAGGTGGAGG CCCTGCTGGC ATCCGAAGAT TATGGCAAAG ACCTAGCTTC TGTGAACAAC 
CTGCTGAAAA AGCATCAACT GCTGGAAGCA GATATATCTG CCCATGAGGA TCGCCTGAAG 
GACCTGAACA GCCAGGCAGA CAGCCTGATG ACCAGCAGTG CCTTCGACAC CTCCCAAGTA 
AAGGACAAGA GGGACACCAT CAACGGGCGC TTCCAGAAGA TCAAGAGCAT GGCGGCCTCC 
CGGCGAGCCA AGCTGAATGA ATCCCATCGC CTGCACCAGT TCTTCCGGGA CATGGATGAC 
GAGGAGTCCT GGATCAAGGA GAAGAAGCTG CTGGTGGGCT CAGAGGACTA CGGCCGGGAC 
CTAACCGGCG TGCAGAACCT GAGGAAGAAG CACAAGCGGC TGGAAGCAGA ACTGGCTGCG 
CATGAGCCGG CTATTCAGGG TGTCCTGGAC ACTGGCAAGA AGCTGTCCGA TGACAACACC 
ATCGGGAAAG AGGAGATCCA GCAGCGGCTG GCGCAGTTTG TGGAGCACTG GAAAGAGCTG 
AAGCAGCTGG CAGCTGCCCG GGGTCAGCGG CTGGAAGAGT CCTTGGAATA TCAGCAGTTT 
GTAGCCAATG TGGAAGAGGA AGAAGCCTGG ATCAATGAGA AAATGACCCT GGTGGCCAGC 
GAAGATTATG GCGACACTCT TGCCGCCATC CAGGGCTTAC TGAAGAAACA TGAAGCTTTT 
GAGACAGACT TCACCGTCCA CAAGGATCGC GTGAATGATG TCTGCACCAA TGGACAAGAC 
CTCATTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CAAAGATGAA GGGCCTGAAC 
GGGAAAGTGT CAGACCTGGA GAAAGCTGCA GCCCAGAGAA AGGCGAAGCT GGATGAGAAC 
TCGGCCTTCC TTCAGTTCAA CTGGAAGGCG GACGTGGTGG AGTCCTGGAT CGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGC CGAGACCTGT CTTCTGTGCA GACGCTCCTC 
ACCAAACAGG AAACTTTTGA CGCTGGGCTG CAGGCCTTCC AGCAGGAAGG CATTGCCAAC 
ATCACTGCCC TCAAAGATCA GCTTCTCGCC GCCAAACACG TTCAGTCCAA GGCCATCGAG 
GCCCGGCACG CCTCCCTCAT GAAGAGGTGG AGCCAGCTTC TGGCCAACTC AGCCGCCCGC 
AAGAAGAAGC TTCTGGAGGC TCAGAGTCAC TTCCGCAAGG TGGAGGACCT CTTCCTGACC 
TTCGCCAAAA AGGCTTCTGC CTTCAACAGC TGGTTTGAAA ATGCAGAGGA GGACTTAACA 
GACCCCGTGC GCTGCAACTC CTTGGAAGAA ATCAAAGCTT TGCGCGAGGC CCACGACGCC 
TTCCGCTCCT CCCTCAGCTC TGCCCAGGCT GACTTCAACC AGCTGGCCGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT AGCCTCCAAC CCCTACACCT GGTTTACCAT GGAGGCCCTG 
GAGGAGACCT GGAGGAACCT ACAGAAAATC ATCAAGGAGA GGGAGCTGGA GCTGCAGAAG 
GAACAGCGGC GGCAGGAGGA GAACGACAAG CTGCGCCAGG AGTTTGCCCA GCACGCCAAC 
GCCTTCCACC AGTGGATCCA AGAGACCAGG ACATACCTCC TCGATGGCAT AGCATATCGT 
CGGGTCATTC GTGTCTATCA GTATGAAGTT GGGGATGATC TGTCTGGAAG GTCCTGTATG 
GTGGAAGAGT CGGGGACCCT CGAATCCCAG CTTGAAGCTA CCAAACGCAA GCACCAGGAA 
ATCCGAGCCA TGAGAAGTCA GCTCAAAAAG ATCGAGGACC TGGGGGCCGC CATGGAGGAG 
GCCCTCATCC TGGACAACAA GTACACGGAG CACAGCACCG TGGGCCTCGC CCAGCAGTGG 
GACCAGCTGG ACCAGCTGGG CATGCGCATG CAGCACAACC TGGAGCAGCA GATCCAGGCC 
AGGAACACAA CAGGTGTGAC TGAGGAGGCC CTCAAAGAAT TCAGCATGAT GTTTAAACAC 
TTTGACAAGG ACAAGTCTGG CAGGCTGAAC CATCAGGAGT TCAAATCTTG CCTGCGCTCC 
CTGGGCTATG ACCTGCCCAT GGTGGAGGAA GGGGAACCTG ACCCTGAGTT CGAGGCAATC 
CTGGACACGG TGGATCCGAA CAGAGATGGC CATGTCTCCT TGCAAGAATA CATGGCTTTC 
ATGATCAGCC GCGAAACTGA GAACGTCAAG TCCAGCGAGG AGATTGAGAG CGCCTTCCGG 
GCCCTCAGCT CAGAGGGAAA GCCTTACGTG ACCAAGGAGG AGCTCTACCA GAACCTGACC 
CGGGAACAAG CCGACTACTG CGTCTCCCAC ATGAAGCCCT ACGTGGACGG CAAGGGCCGC 
GAGCTCCCCA CCGCGTTCGA CTACGTGGAG TTCACCCGCT CGCTTTTCGT GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Critical roles of ?II spectrin in brain development and epileptic encephalopathy.
The Journal of clinical investigation(2018 Jan)
Wang Y,Ji T,Nelson AD,Glanowska K,Murphy GG,Jenkins PM,Parent JM

?II-spectrin in T cells is involved in the regulation of cell-cell contact leading to immunological synapse formation?
PloS one12(12)e0189545(2017)
Meissner JM,Sikorski AF,Nawara T,Grzesiak J,Marycz K,Bogus?awska DM,Michalczyk I,Lecomte MC,Machnicka B

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SPTAN1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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ORF Insert Sequence:

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Target ORF information:

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ORF Insert Sequence:

Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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Target ORF information:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence: