SPTAN1 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_005213345.2	XP_005213402.1	spectrin alpha chain, non-erythrocytic 1 isoform X3
XM_010810457.2	XP_010808759.2	spectrin alpha chain, non-erythrocytic 1 isoform X4
XM_010810459.2	XP_010808761.1	spectrin alpha chain, non-erythrocytic 1 isoform X6
XM_024999028.1	XP_024854796.1	spectrin alpha chain, non-erythrocytic 1 isoform X1
XM_024999030.1	XP_024854798.1	spectrin alpha chain, non-erythrocytic 1 isoform X3
XM_024999033.1	XP_024854801.1	spectrin alpha chain, non-erythrocytic 1 isoform X6
XM_024999044.1	XP_024854812.1	spectrin alpha chain, non-erythrocytic 1 isoform X17
XM_024999046.1	XP_024854814.1	spectrin alpha chain, non-erythrocytic 1 isoform X19
XM_015473653.1	XP_015329139.1	spectrin alpha chain, non-erythrocytic 1 isoform X1
XM_010810456.2	XP_010808758.2	spectrin alpha chain, non-erythrocytic 1 isoform X2
XM_024999034.1	XP_024854802.1	spectrin alpha chain, non-erythrocytic 1 isoform X7
XM_024999038.1	XP_024854806.1	spectrin alpha chain, non-erythrocytic 1 isoform X11
XM_024999039.1	XP_024854807.1	spectrin alpha chain, non-erythrocytic 1 isoform X12
XM_010810458.2	XP_010808760.2	spectrin alpha chain, non-erythrocytic 1 isoform X5
XM_015473654.1	XP_015329140.1	spectrin alpha chain, non-erythrocytic 1 isoform X7
XM_010810462.2	XP_010808764.1	spectrin alpha chain, non-erythrocytic 1 isoform X10
XM_024999035.1	XP_024854803.1	spectrin alpha chain, non-erythrocytic 1 isoform X8
XM_024999036.1	XP_024854804.1	spectrin alpha chain, non-erythrocytic 1 isoform X9
XM_024999037.1	XP_024854805.1	spectrin alpha chain, non-erythrocytic 1 isoform X10
XM_024999041.1	XP_024854809.1	spectrin alpha chain, non-erythrocytic 1 isoform X14
XM_024999042.1	XP_024854810.1	spectrin alpha chain, non-erythrocytic 1 isoform X15
XM_005213345.3	XP_005213402.1	spectrin alpha chain, non-erythrocytic 1 isoform X2
XM_024999031.1	XP_024854799.1	spectrin alpha chain, non-erythrocytic 1 isoform X4
XM_024999032.1	XP_024854800.1	spectrin alpha chain, non-erythrocytic 1 isoform X5
XM_024999043.1	XP_024854811.1	spectrin alpha chain, non-erythrocytic 1 isoform X16
XM_024999045.1	XP_024854813.1	spectrin alpha chain, non-erythrocytic 1 isoform X18
XM_010810460.2	XP_010808762.2	spectrin alpha chain, non-erythrocytic 1 isoform X8
XM_010810461.2	XP_010808763.1	spectrin alpha chain, non-erythrocytic 1 isoform X9
XM_024999029.1	XP_024854797.1	spectrin alpha chain, non-erythrocytic 1 isoform X2
XM_024999040.1	XP_024854808.1	spectrin alpha chain, non-erythrocytic 1 isoform X13
NM_001114156.1	NP_001107628.1	spectrin alpha chain, non-erythrocytic 1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa220568	XM_024999031.1 Latest version!	Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa220569	XM_024999034.1 Latest version!	Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa220570	XM_024999037.1 Latest version!	Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa220571	XM_024999038.1 Latest version!	Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa220572	XM_024999039.1 Latest version!	Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa220573	XM_024999041.1 Latest version!	Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa220574	XM_024999043.1 Latest version!	Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa220575	XM_024999044.1 Latest version!	Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa220576	XM_024999045.1 Latest version!	Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa220577	XM_024999046.1 Latest version!	Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa54548	XM_024999028.1 Latest version!	Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OBa54549	XM_024999030.1 Latest version!	Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OBa54550	XM_024999032.1 Latest version!	Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OBa54552	XM_024999036.1 Latest version!	Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OBa92000	XM_015473653.1 Latest version!	Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OBa92001	XM_010810456.2 Latest version!	Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OBa92002	XM_010810457.2 Latest version!	Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OBa92003	XM_010810458.2 Latest version!	Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OBa92004	XM_010810460.2 Latest version!	Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OBa37323	NM_001114156.1 Latest version!	Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa24457	XM_024999029.1 Latest version!	Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa24457	XM_005213345.3 Latest version!	Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa37323	XM_015473654.1 Latest version!	Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa37323	XM_024999035.1 Latest version!	Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa54551	XM_010810459.2 Latest version!	Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa54551	XM_024999033.1 Latest version!	Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa54553	XM_024999040.1 Latest version!	Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa54553	XM_010810461.2 Latest version!	Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa54554	XM_024999042.1 Latest version!	Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa54554	XM_010810462.2 Latest version!	Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
View Old Accession Versions >>
OBa24457	XM_005213345.2	Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
Hide Old Accession Versions >>

SPTAN1 ( XM_024999031.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854799.1 Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa220568
Clone ID Related Accession (Same CDS sequence)	XM_024999031.1
Accession Version	XM_024999031.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7422bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT 
GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT 
CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC 
AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG 
AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC 
CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG 
AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT 
GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC 
CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC 
CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC 
CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT 
AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT 
GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG 
GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC 
ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC 
TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG 
AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG 
GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA 
GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG 
AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG 
CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG 
GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA 
ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG 
AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT 
GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT 
TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG 
CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT 
GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG 
AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG 
CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT 
CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC 
GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA 
GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG 
GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT 
CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG 
AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC 
AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC 
GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC 
CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC 
GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA 
GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC 
GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC 
AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC 
GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC 
AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGTACC ATTCTCTGCT GGAACTGGGA 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG 
AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC 
GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC 
AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA 
GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC 
AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG 
CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA 
GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC 
TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG 
GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC 
CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA 
TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC 
CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT 
CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG 
TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC 
GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG 
ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG 
GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG 
ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC 
GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG 
ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA 
GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG 
GACCCCACCA ACATCCAGAG CAAGCATCAG AAGCACCAGG CCTTCGAGGC AGAACTGCAC 
GCCAATGCGG ACCGGATCCG TGGGGTCATC GACATGGGCA ACTCCCTCAT TGACCGCGGG 
GCCTGCGCCG GCAGCGAGGA TGCCGTCAAG GCCCGGCTGG CCGCCTTAGC TGACCAGTGG 
CAGTTCCTGG TGCAGAAGTC GGCTGAGAAG AGCCAGAAGC TGAAAGAAGC CAACAAGCAG 
CAGAACTTCA ACACTGGCAT CAAGGACTTC GACTTCTGGC TCTCTGAGGT GGAAGCCCTG 
CTGGCCTCTG AGGATTATGG CAAAGACCTG GCTTCTGTGA ACAACTTGCT AAAAAAACAT 
CAGCTGCTGG AGGCAGATAT CTCTGCCCAC GAGGACCGCC TGAAGGACCT CAACAGCCAG 
GCGGACAGCC TGATGACCAG CAGCGCCTTC GACACCTCCC AGGTGAAGGA CAAGCGGGAC 
GCCATCAACG GCCGTTTCCA GAAGATCAAG AGCATGGCCG CCTCGAGGCG AGCTAGGCTG 
AATGAGTCCC ACCGCCTGCA CCAGTTTTTC CGGGACATGG ATGATGAGGA GTCCTGGATC 
AAGGAAAAGA AGCTGCTGGT GAGCTCAGAG GACTACGGCC GTGACCTGAC GGGCGTCCAG 
AACCTGAGGA AGAAGCACAA ACGGCTGGAA GCAGAGCTGG CTGCCCACGA GCCGGCCATT 
CAGGGTGTCC TGGACACTGG AAAGAAGCTG TCTGATGACA ACACCATCGG GAAGGAGGAG 
ATTCAGCAGC GCCTGGCGCA GTTCGTGGAG CACTGGAAGG AGCTGAAGCA GCTGGCAGCT 
GCCCGGGGCC AGCGGCTAGA AGAATCTTTG GAATATCAGC AGTTTGTAGC CAACGTGGAG 
GAGGAGGAAG CCTGGATCAA TGAGAAGATG ACCCTGGTGG CCAGTGAAGA TTATGGAGAC 
ACTCTTGCTG CCATCCAGGG CTTGCTGAAG AAACACGAAG CCTTCGAGAC GGACTTCACT 
GTCCACAAGG ACCGAGTGAA TGATGTCTGC ACCAACGGCC AGGACCTGGT TAAGAAGAAC 
AATCACCATG AGGAGAACAT CTCTTCCAAG ATGAAGGGCT TGAACGGGAA AGTGTCAGAC 
TTGGAGAAGG CTGCCTCCCA GAGGAAGGCC AAGCTCGACG AGAACTCGGC CTTCCTCCAG 
TTCAACTGGA AAGCCGATGT GGTGGAGTCC TGGATCGGTG AAAAGGAGAA CAGCTTGAAG 
ACAGATGATT ATGGTCGAGA TCTGTCTTCT GTCCAAACTC TCCTCACCAA ACAGGAAACC 
TTCGATGCTG GCCTGCAGGC CTTCCAGCAG GAGGGCATCG CCAACATCAC TGCCCTCAAA 
GATCAGCTGC TGGCCGCCAA GCACGTTCAG TCCAAGGCCA TCGAGGCCCG CCATGCTGCC 
CTCATGAAGA GGTGGAGCCA GCTCCTGGCC AACTCGGCCA CCCGGAAGAA GAAGCTGCTG 
GAGGCACAGA GTCACTTCCG CAAGGTGGAG GATCTCTTCC TGACCTTTGC TAAAAAGGCT 
TCAGCCTTCA ACAGCTGGTT TGAAAACGCA GAGGAGGACT TAACCGACCC AGTGCGCTGC 
AACTCTCTGG AAGAGATCAA GGCCTTGAGA GAGGCCCACG ACGCCTTCCG CTCCTCGTTG 
AGCTCGGCCC AGGCCGACTT CAACCAGCTG GCAGAGCTGG ACCGCCAGAT CAAGAGCTTC 
CGCGTGGCCT CCAACCCCTA CACCTGGTTC ACCATGGAGG CCCTGGAGGA GACCTGGAGA 
AACCTACAGA AGATCATCAA GGAAAGGGAG CTGGAGCTGC AGAAGGAGCA GCGACGGCAG 
GAGGAGAATG ACAAGCTGCG CCAGGAGTTC GCCCAGCATG CCAACGCCTT CCACCAGTGG 
ATCCAGGAGA CCAGAACGTA CCTCCTCGAT GGCATAGCGT ATCGTCGGGT CATTCGTGTC 
TATCAGTATG AAGTTGGGGA TGATCTGTCT GGAAGGTCCT GCATGGTGGA AGAATCCGGG 
ACCCTCGAAT CACAGCTTGA AGCTACCAAA CGCAAACACC AGGAGATTCG AGCTATGAGA 
AGTCAGCTCA AGAAGATTGA AGACCTGGGG GCGGCCATGG AGGAAGCGCT GATCCTGGAC 
AACAAGTACA CGGAGCACAG CACCGTGGGC CTGGCCCAGC AGTGGGACCA GCTCGACCAG 
TTGGGCATGC GCATGCAGCA CAACCTGGAG CAGCAGATCC AGGCCAGGAA CACGACCGGA 
GTGACCGAGG AGGCCCTCAA AGAATTCAGC ATGATGTTCA AACACTTCGA CAAGGACAAG 
TCTGGCCGGC TGAACCACCA GGAGTTCAAA TCCTGTCTGC GCTCCCTGGG CTATGACCTG 
CCCATGGTGG AGGAAGGGGA GCCCGACCCT GAGTTCGAGG CCATCCTGGA CACTGTGGAT 
CCTAACAGGG ACGGCCACGT CTCCCTGCAA GAGTACATGG CCTTTATGAT CAGCCGTGAG 
ACCGAGAACG TCAAGTCCAG CGAGGAGATT GAGAGCGCCT TCCGGGCCCT GAGCTCTGAA 
GGGAAGCCTT ATGTGACCAA GGAGGAGCTG TACCAGAACC TGACTCGGGA ACAAGCCGAC 
TACTGCGTCT CCCACATGAA GCCCTACGTG GACGGCAAGG GCCGCGAACT CCCCACTGCT 
TTTGACTACG TCGAGTTCAC CCGCTCGCTC TTCGTGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854799.1
CDS	4..7425
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLHQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMYRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVDHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWESLKSKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGAMPRDESDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKEKLDVLDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQSFADQLIAGGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQSKHQKHQAFEAELHANADRIRGVIDMGNSLIDRGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDAINGRFQKIKSMAASRRARLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLVKKNNHHEENISSKMKGLNGKVSDLEKAASQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHAALMKRWSQLLANSATRKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGIAYRRVIRVYQYEVGDDLSGRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_024999031.1
Organism	Bos taurus(cattle)
Definition	Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999031.1

ORF Insert Sequence:

ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT 
GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT 
CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC 
AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG 
AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC 
CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG 
AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT 
GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC 
CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC 
CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC 
CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT 
AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT 
GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG 
GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC 
ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC 
TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG 
AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG 
GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA 
GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG 
AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG 
CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG 
GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA 
ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG 
AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT 
GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT 
TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG 
CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT 
GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG 
AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG 
CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT 
CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC 
GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA 
GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG 
GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT 
CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG 
AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC 
AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC 
GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC 
CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC 
GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA 
GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC 
GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC 
AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC 
GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC 
AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGTACC ATTCTCTGCT GGAACTGGGA 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG 
AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC 
GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC 
AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA 
GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC 
AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG 
CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA 
GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC 
TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG 
GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC 
CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA 
TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC 
CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT 
CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG 
TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC 
GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG 
ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG 
GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG 
ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC 
GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG 
ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA 
GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG 
GACCCCACCA ACATCCAGAG CAAGCATCAG AAGCACCAGG CCTTCGAGGC AGAACTGCAC 
GCCAATGCGG ACCGGATCCG TGGGGTCATC GACATGGGCA ACTCCCTCAT TGACCGCGGG 
GCCTGCGCCG GCAGCGAGGA TGCCGTCAAG GCCCGGCTGG CCGCCTTAGC TGACCAGTGG 
CAGTTCCTGG TGCAGAAGTC GGCTGAGAAG AGCCAGAAGC TGAAAGAAGC CAACAAGCAG 
CAGAACTTCA ACACTGGCAT CAAGGACTTC GACTTCTGGC TCTCTGAGGT GGAAGCCCTG 
CTGGCCTCTG AGGATTATGG CAAAGACCTG GCTTCTGTGA ACAACTTGCT AAAAAAACAT 
CAGCTGCTGG AGGCAGATAT CTCTGCCCAC GAGGACCGCC TGAAGGACCT CAACAGCCAG 
GCGGACAGCC TGATGACCAG CAGCGCCTTC GACACCTCCC AGGTGAAGGA CAAGCGGGAC 
GCCATCAACG GCCGTTTCCA GAAGATCAAG AGCATGGCCG CCTCGAGGCG AGCTAGGCTG 
AATGAGTCCC ACCGCCTGCA CCAGTTTTTC CGGGACATGG ATGATGAGGA GTCCTGGATC 
AAGGAAAAGA AGCTGCTGGT GAGCTCAGAG GACTACGGCC GTGACCTGAC GGGCGTCCAG 
AACCTGAGGA AGAAGCACAA ACGGCTGGAA GCAGAGCTGG CTGCCCACGA GCCGGCCATT 
CAGGGTGTCC TGGACACTGG AAAGAAGCTG TCTGATGACA ACACCATCGG GAAGGAGGAG 
ATTCAGCAGC GCCTGGCGCA GTTCGTGGAG CACTGGAAGG AGCTGAAGCA GCTGGCAGCT 
GCCCGGGGCC AGCGGCTAGA AGAATCTTTG GAATATCAGC AGTTTGTAGC CAACGTGGAG 
GAGGAGGAAG CCTGGATCAA TGAGAAGATG ACCCTGGTGG CCAGTGAAGA TTATGGAGAC 
ACTCTTGCTG CCATCCAGGG CTTGCTGAAG AAACACGAAG CCTTCGAGAC GGACTTCACT 
GTCCACAAGG ACCGAGTGAA TGATGTCTGC ACCAACGGCC AGGACCTGGT TAAGAAGAAC 
AATCACCATG AGGAGAACAT CTCTTCCAAG ATGAAGGGCT TGAACGGGAA AGTGTCAGAC 
TTGGAGAAGG CTGCCTCCCA GAGGAAGGCC AAGCTCGACG AGAACTCGGC CTTCCTCCAG 
TTCAACTGGA AAGCCGATGT GGTGGAGTCC TGGATCGGTG AAAAGGAGAA CAGCTTGAAG 
ACAGATGATT ATGGTCGAGA TCTGTCTTCT GTCCAAACTC TCCTCACCAA ACAGGAAACC 
TTCGATGCTG GCCTGCAGGC CTTCCAGCAG GAGGGCATCG CCAACATCAC TGCCCTCAAA 
GATCAGCTGC TGGCCGCCAA GCACGTTCAG TCCAAGGCCA TCGAGGCCCG CCATGCTGCC 
CTCATGAAGA GGTGGAGCCA GCTCCTGGCC AACTCGGCCA CCCGGAAGAA GAAGCTGCTG 
GAGGCACAGA GTCACTTCCG CAAGGTGGAG GATCTCTTCC TGACCTTTGC TAAAAAGGCT 
TCAGCCTTCA ACAGCTGGTT TGAAAACGCA GAGGAGGACT TAACCGACCC AGTGCGCTGC 
AACTCTCTGG AAGAGATCAA GGCCTTGAGA GAGGCCCACG ACGCCTTCCG CTCCTCGTTG 
AGCTCGGCCC AGGCCGACTT CAACCAGCTG GCAGAGCTGG ACCGCCAGAT CAAGAGCTTC 
CGCGTGGCCT CCAACCCCTA CACCTGGTTC ACCATGGAGG CCCTGGAGGA GACCTGGAGA 
AACCTACAGA AGATCATCAA GGAAAGGGAG CTGGAGCTGC AGAAGGAGCA GCGACGGCAG 
GAGGAGAATG ACAAGCTGCG CCAGGAGTTC GCCCAGCATG CCAACGCCTT CCACCAGTGG 
ATCCAGGAGA CCAGAACGTA CCTCCTCGAT GGCATAGCGT ATCGTCGGGT CATTCGTGTC 
TATCAGTATG AAGTTGGGGA TGATCTGTCT GGAAGGTCCT GCATGGTGGA AGAATCCGGG 
ACCCTCGAAT CACAGCTTGA AGCTACCAAA CGCAAACACC AGGAGATTCG AGCTATGAGA 
AGTCAGCTCA AGAAGATTGA AGACCTGGGG GCGGCCATGG AGGAAGCGCT GATCCTGGAC 
AACAAGTACA CGGAGCACAG CACCGTGGGC CTGGCCCAGC AGTGGGACCA GCTCGACCAG 
TTGGGCATGC GCATGCAGCA CAACCTGGAG CAGCAGATCC AGGCCAGGAA CACGACCGGA 
GTGACCGAGG AGGCCCTCAA AGAATTCAGC ATGATGTTCA AACACTTCGA CAAGGACAAG 
TCTGGCCGGC TGAACCACCA GGAGTTCAAA TCCTGTCTGC GCTCCCTGGG CTATGACCTG 
CCCATGGTGG AGGAAGGGGA GCCCGACCCT GAGTTCGAGG CCATCCTGGA CACTGTGGAT 
CCTAACAGGG ACGGCCACGT CTCCCTGCAA GAGTACATGG CCTTTATGAT CAGCCGTGAG 
ACCGAGAACG TCAAGTCCAG CGAGGAGATT GAGAGCGCCT TCCGGGCCCT GAGCTCTGAA 
GGGAAGCCTT ATGTGACCAA GGAGGAGCTG TACCAGAACC TGACTCGGGA ACAAGCCGAC 
TACTGCGTCT CCCACATGAA GCCCTACGTG GACGGCAAGG GCCGCGAACT CCCCACTGCT 
TTTGACTACG TCGAGTTCAC CCGCTCGCTC TTCGTGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_024999034.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854802.1 Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBa220569
Clone ID Related Accession (Same CDS sequence)	XM_024999034.1
Accession Version	XM_024999034.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7386bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT 
GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT 
CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC 
AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG 
AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC 
CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG 
AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT 
GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC 
CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC 
CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC 
CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT 
AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT 
GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG 
GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC 
ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC 
TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG 
AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG 
GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA 
GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG 
AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG 
CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG 
GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA 
ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG 
AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT 
GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT 
TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG 
CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT 
GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG 
AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG 
CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT 
CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC 
GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA 
GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG 
GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT 
CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG 
AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC 
AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC 
GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC 
CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC 
GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA 
GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC 
GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC 
AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC 
GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC 
AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGACGC GCGTAACTAA GGAGGCTGGC 
AGCGTGTCTC TGCGTATGAA ACAGGTGGAA GAACTGTACC ATTCTCTGCT GGAACTGGGA 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG 
AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC 
GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC 
AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA 
GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC 
AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGG AGCTGAACGA GCGCTGGCGG 
TCCCTGCAGC AGCTGGCTGA GGAGCGGAGC CAACTCTTGG GCAGTGCCCA TGAAGTGCAG 
CGGTTCCACA GAGATGCTGA CGAAACCAAA GAGTGGATCG AAGAGAAGAA CCAGGCTCTA 
AACACAGACA ACTACGGCCA CGACCTGGCC AGTGTCCAGG CCCTGCAGCG CAAGCACGAG 
GGCTTCGAGA GGGACCTCGC AGCCCTTGGT GACAAGGTGA ACTCGCTCGG GGAGACAGCC 
GAGCGCCTGA TCCAGTCCCA CCCCGAGTCG GCAGAGGACC TGCAGGAGAA GTGCACTGAG 
CTGAACCAGG CATGGAGCAG CCTGGGCAAG CGTGCCGACC AGCGCAAGGC CAAGCTGGGC 
GACTCCCACG ACCTGCAGCG CTTCCTCAGC GACTTCCGGG ACCTCATGTC TTGGATCAAT 
GGAATCCGAG GTCTGGTGTC CTCAGACGAG CTAGCCAAGG ATGTCACCGG AGCTGAGGCC 
CTGCTGGAGC GACACCAGGA ACACCGGACA GAAATTGATG CCAGAGCTGG CACTTTCCAG 
GCATTCGAGC AGTTTGGGCA GCAGCTGTTG GCTCATGGGC ACTACGCCAG CCCCGAGATC 
AAGGAGAAGC TCGATGTTCT AGACCAGGAG CGTGCAGACC TTGAGAAGGC CTGGGTCCAG 
CGCAGGATGA TGCTGGATCA GTGCCTTGAA TTGCAGCTGT TCCATCGGGA TTGCGAGCAG 
GCCGAGAACT GGATGGCTGC CCGAGAGGCC TTTCTGAACA CAGAAGACAA AGGGGACTCG 
CTAGACAGTG TGGAGGCTCT GATCAAAAAA CACGAGGACT TTGATAAAGC AATTAATGTC 
CAGGAGGAGA AGATTGCTGC CCTGCAGTCC TTTGCTGACC AGCTCATCGC TGGTGGCCAC 
TACGCCAAAG GCGACATCTC CAGCCGGCGC AATGAGGTTC TGGACAGGTG GCGCCGTCTA 
AAAGCTCAGA TGATTGAGAA GAGGTCAAAG CTGGGAGAAT CACAGACCCT CCAGCAGTTC 
AGCCGGGATG TAGACGAGAT TGAAGCTTGG ATCAGTGAAA AATTACAGAC TGCAAGTGAT 
GAGTCATATA AGGACCCCAC CAACATCCAG CTTTCCAAGC TGCTGAGCAA GCATCAGAAG 
CACCAGGCCT TCGAGGCAGA ACTGCACGCC AATGCGGACC GGATCCGTGG GGTCATCGAC 
ATGGGCAACT CCCTCATTGA CCGCGGGGCC TGCGCCGGCA GCGAGGATGC CGTCAAGGCC 
CGGCTGGCCG CCTTAGCTGA CCAGTGGCAG TTCCTGGTGC AGAAGTCGGC TGAGAAGAGC 
CAGAAGCTGA AAGAAGCCAA CAAGCAGCAG AACTTCAACA CTGGCATCAA GGACTTCGAC 
TTCTGGCTCT CTGAGGTGGA AGCCCTGCTG GCCTCTGAGG ATTATGGCAA AGACCTGGCT 
TCTGTGAACA ACTTGCTAAA AAAACATCAG CTGCTGGAGG CAGATATCTC TGCCCACGAG 
GACCGCCTGA AGGACCTCAA CAGCCAGGCG GACAGCCTGA TGACCAGCAG CGCCTTCGAC 
ACCTCCCAGG TGAAGGACAA GCGGGACGCC ATCAACGGCC GTTTCCAGAA GATCAAGAGC 
ATGGCCGCCT CGAGGCGAGC TAGGCTGAAT GAGTCCCACC GCCTGCACCA GTTTTTCCGG 
GACATGGATG ATGAGGAGTC CTGGATCAAG GAAAAGAAGC TGCTGGTGAG CTCAGAGGAC 
TACGGCCGTG ACCTGACGGG CGTCCAGAAC CTGAGGAAGA AGCACAAACG GCTGGAAGCA 
GAGCTGGCTG CCCACGAGCC GGCCATTCAG GGTGTCCTGG ACACTGGAAA GAAGCTGTCT 
GATGACAACA CCATCGGGAA GGAGGAGATT CAGCAGCGCC TGGCGCAGTT CGTGGAGCAC 
TGGAAGGAGC TGAAGCAGCT GGCAGCTGCC CGGGGCCAGC GGCTAGAAGA ATCTTTGGAA 
TATCAGCAGT TTGTAGCCAA CGTGGAGGAG GAGGAAGCCT GGATCAATGA GAAGATGACC 
CTGGTGGCCA GTGAAGATTA TGGAGACACT CTTGCTGCCA TCCAGGGCTT GCTGAAGAAA 
CACGAAGCCT TCGAGACGGA CTTCACTGTC CACAAGGACC GAGTGAATGA TGTCTGCACC 
AACGGCCAGG ACCTGGTTAA GAAGAACAAT CACCATGAGG AGAACATCTC TTCCAAGATG 
AAGGGCTTGA ACGGGAAAGT GTCAGACTTG GAGAAGGCTG CCTCCCAGAG GAAGGCCAAG 
CTCGACGAGA ACTCGGCCTT CCTCCAGTTC AACTGGAAAG CCGATGTGGT GGAGTCCTGG 
ATCGGTGAAA AGGAGAACAG CTTGAAGACA GATGATTATG GTCGAGATCT GTCTTCTGTC 
CAAACTCTCC TCACCAAACA GGAAACCTTC GATGCTGGCC TGCAGGCCTT CCAGCAGGAG 
GGCATCGCCA ACATCACTGC CCTCAAAGAT CAGCTGCTGG CCGCCAAGCA CGTTCAGTCC 
AAGGCCATCG AGGCCCGCCA TGCTGCCCTC ATGAAGAGGT GGAGCCAGCT CCTGGCCAAC 
TCGGCCACCC GGAAGAAGAA GCTGCTGGAG GCACAGAGTC ACTTCCGCAA GGTGGAGGAT 
CTCTTCCTGA CCTTTGCTAA AAAGGCTTCA GCCTTCAACA GCTGGTTTGA AAACGCAGAG 
GAGGACTTAA CCGACCCAGT GCGCTGCAAC TCTCTGGAAG AGATCAAGGC CTTGAGAGAG 
GCCCACGACG CCTTCCGCTC CTCGTTGAGC TCGGCCCAGG CCGACTTCAA CCAGCTGGCA 
GAGCTGGACC GCCAGATCAA GAGCTTCCGC GTGGCCTCCA ACCCCTACAC CTGGTTCACC 
ATGGAGGCCC TGGAGGAGAC CTGGAGAAAC CTACAGAAGA TCATCAAGGA AAGGGAGCTG 
GAGCTGCAGA AGGAGCAGCG ACGGCAGGAG GAGAATGACA AGCTGCGCCA GGAGTTCGCC 
CAGCATGCCA ACGCCTTCCA CCAGTGGATC CAGGAGACCA GAACGTACCT CCTCGATGGG 
TCCTGCATGG TGGAAGAATC CGGGACCCTC GAATCACAGC TTGAAGCTAC CAAACGCAAA 
CACCAGGAGA TTCGAGCTAT GAGAAGTCAG CTCAAGAAGA TTGAAGACCT GGGGGCGGCC 
ATGGAGGAAG CGCTGATCCT GGACAACAAG TACACGGAGC ACAGCACCGT GGGCCTGGCC 
CAGCAGTGGG ACCAGCTCGA CCAGTTGGGC ATGCGCATGC AGCACAACCT GGAGCAGCAG 
ATCCAGGCCA GGAACACGAC CGGAGTGACC GAGGAGGCCC TCAAAGAATT CAGCATGATG 
TTCAAACACT TCGACAAGGA CAAGTCTGGC CGGCTGAACC ACCAGGAGTT CAAATCCTGT 
CTGCGCTCCC TGGGCTATGA CCTGCCCATG GTGGAGGAAG GGGAGCCCGA CCCTGAGTTC 
GAGGCCATCC TGGACACTGT GGATCCTAAC AGGGACGGCC ACGTCTCCCT GCAAGAGTAC 
ATGGCCTTTA TGATCAGCCG TGAGACCGAG AACGTCAAGT CCAGCGAGGA GATTGAGAGC 
GCCTTCCGGG CCCTGAGCTC TGAAGGGAAG CCTTATGTGA CCAAGGAGGA GCTGTACCAG 
AACCTGACTC GGGAACAAGC CGACTACTGC GTCTCCCACA TGAAGCCCTA CGTGGACGGC 
AAGGGCCGCG AACTCCCCAC TGCTTTTGAC TACGTCGAGT TCACCCGCTC GCTCTTCGTG 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854802.1
CDS	4..7389
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLHQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMYRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVDHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWESLKSKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRVTKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGAMPRDESDSKTASPWKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKEKLDVLDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQSFADQLIAGGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDMGNSLIDRGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDAINGRFQKIKSMAASRRARLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLVKKNNHHEENISSKMKGLNGKVSDLEKAASQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHAALMKRWSQLLANSATRKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_024999034.1
Organism	Bos taurus(cattle)
Definition	Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999034.1

ORF Insert Sequence:

ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT 
GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT 
CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC 
AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG 
AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC 
CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG 
AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT 
GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC 
CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC 
CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC 
CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT 
AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT 
GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG 
GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC 
ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC 
TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG 
AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG 
GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA 
GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG 
AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG 
CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG 
GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA 
ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG 
AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT 
GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT 
TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG 
CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT 
GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG 
AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG 
CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT 
CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC 
GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA 
GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG 
GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT 
CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG 
AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC 
AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC 
GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC 
CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC 
GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA 
GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC 
GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC 
AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC 
GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC 
AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGACGC GCGTAACTAA GGAGGCTGGC 
AGCGTGTCTC TGCGTATGAA ACAGGTGGAA GAACTGTACC ATTCTCTGCT GGAACTGGGA 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG 
AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC 
GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC 
AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA 
GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC 
AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGG AGCTGAACGA GCGCTGGCGG 
TCCCTGCAGC AGCTGGCTGA GGAGCGGAGC CAACTCTTGG GCAGTGCCCA TGAAGTGCAG 
CGGTTCCACA GAGATGCTGA CGAAACCAAA GAGTGGATCG AAGAGAAGAA CCAGGCTCTA 
AACACAGACA ACTACGGCCA CGACCTGGCC AGTGTCCAGG CCCTGCAGCG CAAGCACGAG 
GGCTTCGAGA GGGACCTCGC AGCCCTTGGT GACAAGGTGA ACTCGCTCGG GGAGACAGCC 
GAGCGCCTGA TCCAGTCCCA CCCCGAGTCG GCAGAGGACC TGCAGGAGAA GTGCACTGAG 
CTGAACCAGG CATGGAGCAG CCTGGGCAAG CGTGCCGACC AGCGCAAGGC CAAGCTGGGC 
GACTCCCACG ACCTGCAGCG CTTCCTCAGC GACTTCCGGG ACCTCATGTC TTGGATCAAT 
GGAATCCGAG GTCTGGTGTC CTCAGACGAG CTAGCCAAGG ATGTCACCGG AGCTGAGGCC 
CTGCTGGAGC GACACCAGGA ACACCGGACA GAAATTGATG CCAGAGCTGG CACTTTCCAG 
GCATTCGAGC AGTTTGGGCA GCAGCTGTTG GCTCATGGGC ACTACGCCAG CCCCGAGATC 
AAGGAGAAGC TCGATGTTCT AGACCAGGAG CGTGCAGACC TTGAGAAGGC CTGGGTCCAG 
CGCAGGATGA TGCTGGATCA GTGCCTTGAA TTGCAGCTGT TCCATCGGGA TTGCGAGCAG 
GCCGAGAACT GGATGGCTGC CCGAGAGGCC TTTCTGAACA CAGAAGACAA AGGGGACTCG 
CTAGACAGTG TGGAGGCTCT GATCAAAAAA CACGAGGACT TTGATAAAGC AATTAATGTC 
CAGGAGGAGA AGATTGCTGC CCTGCAGTCC TTTGCTGACC AGCTCATCGC TGGTGGCCAC 
TACGCCAAAG GCGACATCTC CAGCCGGCGC AATGAGGTTC TGGACAGGTG GCGCCGTCTA 
AAAGCTCAGA TGATTGAGAA GAGGTCAAAG CTGGGAGAAT CACAGACCCT CCAGCAGTTC 
AGCCGGGATG TAGACGAGAT TGAAGCTTGG ATCAGTGAAA AATTACAGAC TGCAAGTGAT 
GAGTCATATA AGGACCCCAC CAACATCCAG CTTTCCAAGC TGCTGAGCAA GCATCAGAAG 
CACCAGGCCT TCGAGGCAGA ACTGCACGCC AATGCGGACC GGATCCGTGG GGTCATCGAC 
ATGGGCAACT CCCTCATTGA CCGCGGGGCC TGCGCCGGCA GCGAGGATGC CGTCAAGGCC 
CGGCTGGCCG CCTTAGCTGA CCAGTGGCAG TTCCTGGTGC AGAAGTCGGC TGAGAAGAGC 
CAGAAGCTGA AAGAAGCCAA CAAGCAGCAG AACTTCAACA CTGGCATCAA GGACTTCGAC 
TTCTGGCTCT CTGAGGTGGA AGCCCTGCTG GCCTCTGAGG ATTATGGCAA AGACCTGGCT 
TCTGTGAACA ACTTGCTAAA AAAACATCAG CTGCTGGAGG CAGATATCTC TGCCCACGAG 
GACCGCCTGA AGGACCTCAA CAGCCAGGCG GACAGCCTGA TGACCAGCAG CGCCTTCGAC 
ACCTCCCAGG TGAAGGACAA GCGGGACGCC ATCAACGGCC GTTTCCAGAA GATCAAGAGC 
ATGGCCGCCT CGAGGCGAGC TAGGCTGAAT GAGTCCCACC GCCTGCACCA GTTTTTCCGG 
GACATGGATG ATGAGGAGTC CTGGATCAAG GAAAAGAAGC TGCTGGTGAG CTCAGAGGAC 
TACGGCCGTG ACCTGACGGG CGTCCAGAAC CTGAGGAAGA AGCACAAACG GCTGGAAGCA 
GAGCTGGCTG CCCACGAGCC GGCCATTCAG GGTGTCCTGG ACACTGGAAA GAAGCTGTCT 
GATGACAACA CCATCGGGAA GGAGGAGATT CAGCAGCGCC TGGCGCAGTT CGTGGAGCAC 
TGGAAGGAGC TGAAGCAGCT GGCAGCTGCC CGGGGCCAGC GGCTAGAAGA ATCTTTGGAA 
TATCAGCAGT TTGTAGCCAA CGTGGAGGAG GAGGAAGCCT GGATCAATGA GAAGATGACC 
CTGGTGGCCA GTGAAGATTA TGGAGACACT CTTGCTGCCA TCCAGGGCTT GCTGAAGAAA 
CACGAAGCCT TCGAGACGGA CTTCACTGTC CACAAGGACC GAGTGAATGA TGTCTGCACC 
AACGGCCAGG ACCTGGTTAA GAAGAACAAT CACCATGAGG AGAACATCTC TTCCAAGATG 
AAGGGCTTGA ACGGGAAAGT GTCAGACTTG GAGAAGGCTG CCTCCCAGAG GAAGGCCAAG 
CTCGACGAGA ACTCGGCCTT CCTCCAGTTC AACTGGAAAG CCGATGTGGT GGAGTCCTGG 
ATCGGTGAAA AGGAGAACAG CTTGAAGACA GATGATTATG GTCGAGATCT GTCTTCTGTC 
CAAACTCTCC TCACCAAACA GGAAACCTTC GATGCTGGCC TGCAGGCCTT CCAGCAGGAG 
GGCATCGCCA ACATCACTGC CCTCAAAGAT CAGCTGCTGG CCGCCAAGCA CGTTCAGTCC 
AAGGCCATCG AGGCCCGCCA TGCTGCCCTC ATGAAGAGGT GGAGCCAGCT CCTGGCCAAC 
TCGGCCACCC GGAAGAAGAA GCTGCTGGAG GCACAGAGTC ACTTCCGCAA GGTGGAGGAT 
CTCTTCCTGA CCTTTGCTAA AAAGGCTTCA GCCTTCAACA GCTGGTTTGA AAACGCAGAG 
GAGGACTTAA CCGACCCAGT GCGCTGCAAC TCTCTGGAAG AGATCAAGGC CTTGAGAGAG 
GCCCACGACG CCTTCCGCTC CTCGTTGAGC TCGGCCCAGG CCGACTTCAA CCAGCTGGCA 
GAGCTGGACC GCCAGATCAA GAGCTTCCGC GTGGCCTCCA ACCCCTACAC CTGGTTCACC 
ATGGAGGCCC TGGAGGAGAC CTGGAGAAAC CTACAGAAGA TCATCAAGGA AAGGGAGCTG 
GAGCTGCAGA AGGAGCAGCG ACGGCAGGAG GAGAATGACA AGCTGCGCCA GGAGTTCGCC 
CAGCATGCCA ACGCCTTCCA CCAGTGGATC CAGGAGACCA GAACGTACCT CCTCGATGGG 
TCCTGCATGG TGGAAGAATC CGGGACCCTC GAATCACAGC TTGAAGCTAC CAAACGCAAA 
CACCAGGAGA TTCGAGCTAT GAGAAGTCAG CTCAAGAAGA TTGAAGACCT GGGGGCGGCC 
ATGGAGGAAG CGCTGATCCT GGACAACAAG TACACGGAGC ACAGCACCGT GGGCCTGGCC 
CAGCAGTGGG ACCAGCTCGA CCAGTTGGGC ATGCGCATGC AGCACAACCT GGAGCAGCAG 
ATCCAGGCCA GGAACACGAC CGGAGTGACC GAGGAGGCCC TCAAAGAATT CAGCATGATG 
TTCAAACACT TCGACAAGGA CAAGTCTGGC CGGCTGAACC ACCAGGAGTT CAAATCCTGT 
CTGCGCTCCC TGGGCTATGA CCTGCCCATG GTGGAGGAAG GGGAGCCCGA CCCTGAGTTC 
GAGGCCATCC TGGACACTGT GGATCCTAAC AGGGACGGCC ACGTCTCCCT GCAAGAGTAC 
ATGGCCTTTA TGATCAGCCG TGAGACCGAG AACGTCAAGT CCAGCGAGGA GATTGAGAGC 
GCCTTCCGGG CCCTGAGCTC TGAAGGGAAG CCTTATGTGA CCAAGGAGGA GCTGTACCAG 
AACCTGACTC GGGAACAAGC CGACTACTGC GTCTCCCACA TGAAGCCCTA CGTGGACGGC 
AAGGGCCGCG AACTCCCCAC TGCTTTTGAC TACGTCGAGT TCACCCGCTC GCTCTTCGTG 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_024999037.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854805.1 Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OBa220570
Clone ID Related Accession (Same CDS sequence)	XM_024999037.1
Accession Version	XM_024999037.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7401bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT 
GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT 
CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC 
AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG 
AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC 
CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG 
AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT 
GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC 
CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC 
CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC 
CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT 
AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT 
GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG 
GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC 
ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC 
TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG 
AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG 
GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA 
GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG 
AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG 
CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG 
GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA 
ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG 
AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT 
GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT 
TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG 
CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT 
GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG 
AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG 
CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT 
CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC 
GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA 
GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG 
GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT 
CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG 
AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC 
AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC 
GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC 
CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC 
GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA 
GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC 
GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC 
AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC 
GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC 
AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGACGC GCGTAACTAA GGAGGCTGGC 
AGCGTGTCTC TGCGTATGAA ACAGGTGGAA GAACTGTACC ATTCTCTGCT GGAACTGGGA 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG 
AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC 
GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC 
AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA 
GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC 
AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG 
CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA 
GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC 
TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG 
GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC 
CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA 
TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC 
CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT 
CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG 
TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC 
GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG 
ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG 
GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG 
ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC 
GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG 
ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA 
GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG 
GACCCCACCA ACATCCAGAG CAAGCATCAG AAGCACCAGG CCTTCGAGGC AGAACTGCAC 
GCCAATGCGG ACCGGATCCG TGGGGTCATC GACATGGGCA ACTCCCTCAT TGACCGCGGG 
GCCTGCGCCG GCAGCGAGGA TGCCGTCAAG GCCCGGCTGG CCGCCTTAGC TGACCAGTGG 
CAGTTCCTGG TGCAGAAGTC GGCTGAGAAG AGCCAGAAGC TGAAAGAAGC CAACAAGCAG 
CAGAACTTCA ACACTGGCAT CAAGGACTTC GACTTCTGGC TCTCTGAGGT GGAAGCCCTG 
CTGGCCTCTG AGGATTATGG CAAAGACCTG GCTTCTGTGA ACAACTTGCT AAAAAAACAT 
CAGCTGCTGG AGGCAGATAT CTCTGCCCAC GAGGACCGCC TGAAGGACCT CAACAGCCAG 
GCGGACAGCC TGATGACCAG CAGCGCCTTC GACACCTCCC AGGTGAAGGA CAAGCGGGAC 
GCCATCAACG GCCGTTTCCA GAAGATCAAG AGCATGGCCG CCTCGAGGCG AGCTAGGCTG 
AATGAGTCCC ACCGCCTGCA CCAGTTTTTC CGGGACATGG ATGATGAGGA GTCCTGGATC 
AAGGAAAAGA AGCTGCTGGT GAGCTCAGAG GACTACGGCC GTGACCTGAC GGGCGTCCAG 
AACCTGAGGA AGAAGCACAA ACGGCTGGAA GCAGAGCTGG CTGCCCACGA GCCGGCCATT 
CAGGGTGTCC TGGACACTGG AAAGAAGCTG TCTGATGACA ACACCATCGG GAAGGAGGAG 
ATTCAGCAGC GCCTGGCGCA GTTCGTGGAG CACTGGAAGG AGCTGAAGCA GCTGGCAGCT 
GCCCGGGGCC AGCGGCTAGA AGAATCTTTG GAATATCAGC AGTTTGTAGC CAACGTGGAG 
GAGGAGGAAG CCTGGATCAA TGAGAAGATG ACCCTGGTGG CCAGTGAAGA TTATGGAGAC 
ACTCTTGCTG CCATCCAGGG CTTGCTGAAG AAACACGAAG CCTTCGAGAC GGACTTCACT 
GTCCACAAGG ACCGAGTGAA TGATGTCTGC ACCAACGGCC AGGACCTGGT TAAGAAGAAC 
AATCACCATG AGGAGAACAT CTCTTCCAAG ATGAAGGGCT TGAACGGGAA AGTGTCAGAC 
TTGGAGAAGG CTGCCTCCCA GAGGAAGGCC AAGCTCGACG AGAACTCGGC CTTCCTCCAG 
TTCAACTGGA AAGCCGATGT GGTGGAGTCC TGGATCGGTG AAAAGGAGAA CAGCTTGAAG 
ACAGATGATT ATGGTCGAGA TCTGTCTTCT GTCCAAACTC TCCTCACCAA ACAGGAAACC 
TTCGATGCTG GCCTGCAGGC CTTCCAGCAG GAGGGCATCG CCAACATCAC TGCCCTCAAA 
GATCAGCTGC TGGCCGCCAA GCACGTTCAG TCCAAGGCCA TCGAGGCCCG CCATGCTGCC 
CTCATGAAGA GGTGGAGCCA GCTCCTGGCC AACTCGGCCA CCCGGAAGAA GAAGCTGCTG 
GAGGCACAGA GTCACTTCCG CAAGGTGGAG GATCTCTTCC TGACCTTTGC TAAAAAGGCT 
TCAGCCTTCA ACAGCTGGTT TGAAAACGCA GAGGAGGACT TAACCGACCC AGTGCGCTGC 
AACTCTCTGG AAGAGATCAA GGCCTTGAGA GAGGCCCACG ACGCCTTCCG CTCCTCGTTG 
AGCTCGGCCC AGGCCGACTT CAACCAGCTG GCAGAGCTGG ACCGCCAGAT CAAGAGCTTC 
CGCGTGGCCT CCAACCCCTA CACCTGGTTC ACCATGGAGG CCCTGGAGGA GACCTGGAGA 
AACCTACAGA AGATCATCAA GGAAAGGGAG CTGGAGCTGC AGAAGGAGCA GCGACGGCAG 
GAGGAGAATG ACAAGCTGCG CCAGGAGTTC GCCCAGCATG CCAACGCCTT CCACCAGTGG 
ATCCAGGAGA CCAGGTCCTG CATGGTGGAA GAATCCGGGA CCCTCGAATC ACAGCTTGAA 
GCTACCAAAC GCAAACACCA GGAGATTCGA GCTATGAGAA GTCAGCTCAA GAAGATTGAA 
GACCTGGGGG CGGCCATGGA GGAAGCGCTG ATCCTGGACA ACAAGTACAC GGAGCACAGC 
ACCGTGGGCC TGGCCCAGCA GTGGGACCAG CTCGACCAGT TGGGCATGCG CATGCAGCAC 
AACCTGGAGC AGCAGATCCA GGCCAGGAAC ACGACCGGAG TGACCGAGGA GGCCCTCAAA 
GAATTCAGCA TGATGTTCAA ACACTTCGAC AAGGACAAGT CTGGCCGGCT GAACCACCAG 
GAGTTCAAAT CCTGTCTGCG CTCCCTGGGC TATGACCTGC CCATGGTGGA GGAAGGGGAG 
CCCGACCCTG AGTTCGAGGC CATCCTGGAC ACTGTGGATC CTAACAGGGA CGGCCACGTC 
TCCCTGCAAG AGTACATGGC CTTTATGATC AGCCGTGAGA CCGAGAACGT CAAGTCCAGC 
GAGGAGATTG AGAGCGCCTT CCGGGCCCTG AGCTCTGAAG GGAAGCCTTA TGTGACCAAG 
GAGGAGCTGT ACCAGAACCT GACTCGGGAA CAAGCCGACT ACTGCGTCTC CCACATGAAG 
CCCTACGTGG ACGGCAAGGG CCGCGAACTC CCCACTGCTT TTGACTACGT CGAGTTCACC 
CGCTCGCTCT TCGTGAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854805.1
CDS	120..7520
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLHQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMYRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVDHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWESLKSKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRVTKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGAMPRDESDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKEKLDVLDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQSFADQLIAGGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQSKHQKHQAFEAELHANADRIRGVIDMGNSLIDRGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDAINGRFQKIKSMAASRRARLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLVKKNNHHEENISSKMKGLNGKVSDLEKAASQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHAALMKRWSQLLANSATRKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_024999037.1
Organism	Bos taurus(cattle)
Definition	Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999037.1

ORF Insert Sequence:

ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT 
GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT 
CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC 
AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG 
AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC 
CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG 
AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT 
GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC 
CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC 
CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC 
CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT 
AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT 
GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG 
GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC 
ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC 
TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG 
AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG 
GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA 
GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG 
AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG 
CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG 
GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA 
ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG 
AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT 
GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT 
TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG 
CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT 
GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG 
AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG 
CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT 
CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC 
GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA 
GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG 
GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT 
CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG 
AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC 
AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC 
GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC 
CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC 
GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA 
GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC 
GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC 
AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC 
GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC 
AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGACGC GCGTAACTAA GGAGGCTGGC 
AGCGTGTCTC TGCGTATGAA ACAGGTGGAA GAACTGTACC ATTCTCTGCT GGAACTGGGA 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG 
AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC 
GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC 
AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA 
GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC 
AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG 
CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA 
GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC 
TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG 
GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC 
CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA 
TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC 
CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT 
CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG 
TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC 
GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG 
ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG 
GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG 
ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC 
GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG 
ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA 
GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG 
GACCCCACCA ACATCCAGAG CAAGCATCAG AAGCACCAGG CCTTCGAGGC AGAACTGCAC 
GCCAATGCGG ACCGGATCCG TGGGGTCATC GACATGGGCA ACTCCCTCAT TGACCGCGGG 
GCCTGCGCCG GCAGCGAGGA TGCCGTCAAG GCCCGGCTGG CCGCCTTAGC TGACCAGTGG 
CAGTTCCTGG TGCAGAAGTC GGCTGAGAAG AGCCAGAAGC TGAAAGAAGC CAACAAGCAG 
CAGAACTTCA ACACTGGCAT CAAGGACTTC GACTTCTGGC TCTCTGAGGT GGAAGCCCTG 
CTGGCCTCTG AGGATTATGG CAAAGACCTG GCTTCTGTGA ACAACTTGCT AAAAAAACAT 
CAGCTGCTGG AGGCAGATAT CTCTGCCCAC GAGGACCGCC TGAAGGACCT CAACAGCCAG 
GCGGACAGCC TGATGACCAG CAGCGCCTTC GACACCTCCC AGGTGAAGGA CAAGCGGGAC 
GCCATCAACG GCCGTTTCCA GAAGATCAAG AGCATGGCCG CCTCGAGGCG AGCTAGGCTG 
AATGAGTCCC ACCGCCTGCA CCAGTTTTTC CGGGACATGG ATGATGAGGA GTCCTGGATC 
AAGGAAAAGA AGCTGCTGGT GAGCTCAGAG GACTACGGCC GTGACCTGAC GGGCGTCCAG 
AACCTGAGGA AGAAGCACAA ACGGCTGGAA GCAGAGCTGG CTGCCCACGA GCCGGCCATT 
CAGGGTGTCC TGGACACTGG AAAGAAGCTG TCTGATGACA ACACCATCGG GAAGGAGGAG 
ATTCAGCAGC GCCTGGCGCA GTTCGTGGAG CACTGGAAGG AGCTGAAGCA GCTGGCAGCT 
GCCCGGGGCC AGCGGCTAGA AGAATCTTTG GAATATCAGC AGTTTGTAGC CAACGTGGAG 
GAGGAGGAAG CCTGGATCAA TGAGAAGATG ACCCTGGTGG CCAGTGAAGA TTATGGAGAC 
ACTCTTGCTG CCATCCAGGG CTTGCTGAAG AAACACGAAG CCTTCGAGAC GGACTTCACT 
GTCCACAAGG ACCGAGTGAA TGATGTCTGC ACCAACGGCC AGGACCTGGT TAAGAAGAAC 
AATCACCATG AGGAGAACAT CTCTTCCAAG ATGAAGGGCT TGAACGGGAA AGTGTCAGAC 
TTGGAGAAGG CTGCCTCCCA GAGGAAGGCC AAGCTCGACG AGAACTCGGC CTTCCTCCAG 
TTCAACTGGA AAGCCGATGT GGTGGAGTCC TGGATCGGTG AAAAGGAGAA CAGCTTGAAG 
ACAGATGATT ATGGTCGAGA TCTGTCTTCT GTCCAAACTC TCCTCACCAA ACAGGAAACC 
TTCGATGCTG GCCTGCAGGC CTTCCAGCAG GAGGGCATCG CCAACATCAC TGCCCTCAAA 
GATCAGCTGC TGGCCGCCAA GCACGTTCAG TCCAAGGCCA TCGAGGCCCG CCATGCTGCC 
CTCATGAAGA GGTGGAGCCA GCTCCTGGCC AACTCGGCCA CCCGGAAGAA GAAGCTGCTG 
GAGGCACAGA GTCACTTCCG CAAGGTGGAG GATCTCTTCC TGACCTTTGC TAAAAAGGCT 
TCAGCCTTCA ACAGCTGGTT TGAAAACGCA GAGGAGGACT TAACCGACCC AGTGCGCTGC 
AACTCTCTGG AAGAGATCAA GGCCTTGAGA GAGGCCCACG ACGCCTTCCG CTCCTCGTTG 
AGCTCGGCCC AGGCCGACTT CAACCAGCTG GCAGAGCTGG ACCGCCAGAT CAAGAGCTTC 
CGCGTGGCCT CCAACCCCTA CACCTGGTTC ACCATGGAGG CCCTGGAGGA GACCTGGAGA 
AACCTACAGA AGATCATCAA GGAAAGGGAG CTGGAGCTGC AGAAGGAGCA GCGACGGCAG 
GAGGAGAATG ACAAGCTGCG CCAGGAGTTC GCCCAGCATG CCAACGCCTT CCACCAGTGG 
ATCCAGGAGA CCAGGTCCTG CATGGTGGAA GAATCCGGGA CCCTCGAATC ACAGCTTGAA 
GCTACCAAAC GCAAACACCA GGAGATTCGA GCTATGAGAA GTCAGCTCAA GAAGATTGAA 
GACCTGGGGG CGGCCATGGA GGAAGCGCTG ATCCTGGACA ACAAGTACAC GGAGCACAGC 
ACCGTGGGCC TGGCCCAGCA GTGGGACCAG CTCGACCAGT TGGGCATGCG CATGCAGCAC 
AACCTGGAGC AGCAGATCCA GGCCAGGAAC ACGACCGGAG TGACCGAGGA GGCCCTCAAA 
GAATTCAGCA TGATGTTCAA ACACTTCGAC AAGGACAAGT CTGGCCGGCT GAACCACCAG 
GAGTTCAAAT CCTGTCTGCG CTCCCTGGGC TATGACCTGC CCATGGTGGA GGAAGGGGAG 
CCCGACCCTG AGTTCGAGGC CATCCTGGAC ACTGTGGATC CTAACAGGGA CGGCCACGTC 
TCCCTGCAAG AGTACATGGC CTTTATGATC AGCCGTGAGA CCGAGAACGT CAAGTCCAGC 
GAGGAGATTG AGAGCGCCTT CCGGGCCCTG AGCTCTGAAG GGAAGCCTTA TGTGACCAAG 
GAGGAGCTGT ACCAGAACCT GACTCGGGAA CAAGCCGACT ACTGCGTCTC CCACATGAAG 
CCCTACGTGG ACGGCAAGGG CCGCGAACTC CCCACTGCTT TTGACTACGT CGAGTTCACC 
CGCTCGCTCT TCGTGAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_024999038.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854806.1 Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OBa220571
Clone ID Related Accession (Same CDS sequence)	XM_024999038.1
Accession Version	XM_024999038.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7356bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT 
GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT 
CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC 
AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG 
AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC 
CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG 
AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT 
GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC 
CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC 
CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC 
CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT 
AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT 
GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG 
GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC 
ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC 
TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG 
AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG 
GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA 
GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG 
AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG 
CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG 
GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA 
ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG 
AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT 
GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT 
TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG 
CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT 
GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG 
AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG 
CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT 
CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC 
GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA 
GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG 
GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT 
CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG 
AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC 
AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC 
GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC 
CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC 
GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA 
GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC 
GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC 
AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC 
GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC 
AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGTACC ATTCTCTGCT GGAACTGGGA 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG 
AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC 
GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC 
AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA 
GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC 
AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG 
CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA 
GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC 
TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG 
GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC 
CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA 
TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC 
CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT 
CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG 
TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC 
GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG 
ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG 
GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG 
ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC 
GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG 
ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA 
GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG 
GACCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ATCAGAAGCA CCAGGCCTTC 
GAGGCAGAAC TGCACGCCAA TGCGGACCGG ATCCGTGGGG TCATCGACAT GGGCAACTCC 
CTCATTGACC GCGGGGCCTG CGCCGGCAGC GAGGATGCCG TCAAGGCCCG GCTGGCCGCC 
TTAGCTGACC AGTGGCAGTT CCTGGTGCAG AAGTCGGCTG AGAAGAGCCA GAAGCTGAAA 
GAAGCCAACA AGCAGCAGAA CTTCAACACT GGCATCAAGG ACTTCGACTT CTGGCTCTCT 
GAGGTGGAAG CCCTGCTGGC CTCTGAGGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC 
TTGCTAAAAA AACATCAGCT GCTGGAGGCA GATATCTCTG CCCACGAGGA CCGCCTGAAG 
GACCTCAACA GCCAGGCGGA CAGCCTGATG ACCAGCAGCG CCTTCGACAC CTCCCAGGTG 
AAGGACAAGC GGGACGCCAT CAACGGCCGT TTCCAGAAGA TCAAGAGCAT GGCCGCCTCG 
AGGCGAGCTA GGCTGAATGA GTCCCACCGC CTGCACCAGT TTTTCCGGGA CATGGATGAT 
GAGGAGTCCT GGATCAAGGA AAAGAAGCTG CTGGTGAGCT CAGAGGACTA CGGCCGTGAC 
CTGACGGGCG TCCAGAACCT GAGGAAGAAG CACAAACGGC TGGAAGCAGA GCTGGCTGCC 
CACGAGCCGG CCATTCAGGG TGTCCTGGAC ACTGGAAAGA AGCTGTCTGA TGACAACACC 
ATCGGGAAGG AGGAGATTCA GCAGCGCCTG GCGCAGTTCG TGGAGCACTG GAAGGAGCTG 
AAGCAGCTGG CAGCTGCCCG GGGCCAGCGG CTAGAAGAAT CTTTGGAATA TCAGCAGTTT 
GTAGCCAACG TGGAGGAGGA GGAAGCCTGG ATCAATGAGA AGATGACCCT GGTGGCCAGT 
GAAGATTATG GAGACACTCT TGCTGCCATC CAGGGCTTGC TGAAGAAACA CGAAGCCTTC 
GAGACGGACT TCACTGTCCA CAAGGACCGA GTGAATGATG TCTGCACCAA CGGCCAGGAC 
CTGGTTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CCAAGATGAA GGGCTTGAAC 
GGGAAAGTGT CAGACTTGGA GAAGGCTGCC TCCCAGAGGA AGGCCAAGCT CGACGAGAAC 
TCGGCCTTCC TCCAGTTCAA CTGGAAAGCC GATGTGGTGG AGTCCTGGAT CGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGT CGAGATCTGT CTTCTGTCCA AACTCTCCTC 
ACCAAACAGG AAACCTTCGA TGCTGGCCTG CAGGCCTTCC AGCAGGAGGG CATCGCCAAC 
ATCACTGCCC TCAAAGATCA GCTGCTGGCC GCCAAGCACG TTCAGTCCAA GGCCATCGAG 
GCCCGCCATG CTGCCCTCAT GAAGAGGTGG AGCCAGCTCC TGGCCAACTC GGCCACCCGG 
AAGAAGAAGC TGCTGGAGGC ACAGAGTCAC TTCCGCAAGG TGGAGGATCT CTTCCTGACC 
TTTGCTAAAA AGGCTTCAGC CTTCAACAGC TGGTTTGAAA ACGCAGAGGA GGACTTAACC 
GACCCAGTGC GCTGCAACTC TCTGGAAGAG ATCAAGGCCT TGAGAGAGGC CCACGACGCC 
TTCCGCTCCT CGTTGAGCTC GGCCCAGGCC GACTTCAACC AGCTGGCAGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT GGCCTCCAAC CCCTACACCT GGTTCACCAT GGAGGCCCTG 
GAGGAGACCT GGAGAAACCT ACAGAAGATC ATCAAGGAAA GGGAGCTGGA GCTGCAGAAG 
GAGCAGCGAC GGCAGGAGGA GAATGACAAG CTGCGCCAGG AGTTCGCCCA GCATGCCAAC 
GCCTTCCACC AGTGGATCCA GGAGACCAGG TCCTGCATGG TGGAAGAATC CGGGACCCTC 
GAATCACAGC TTGAAGCTAC CAAACGCAAA CACCAGGAGA TTCGAGCTAT GAGAAGTCAG 
CTCAAGAAGA TTGAAGACCT GGGGGCGGCC ATGGAGGAAG CGCTGATCCT GGACAACAAG 
TACACGGAGC ACAGCACCGT GGGCCTGGCC CAGCAGTGGG ACCAGCTCGA CCAGTTGGGC 
ATGCGCATGC AGCACAACCT GGAGCAGCAG ATCCAGGCCA GGAACACGAC CGGAGTGACC 
GAGGAGGCCC TCAAAGAATT CAGCATGATG TTCAAACACT TCGACAAGGA CAAGTCTGGC 
CGGCTGAACC ACCAGGAGTT CAAATCCTGT CTGCGCTCCC TGGGCTATGA CCTGCCCATG 
GTGGAGGAAG GGGAGCCCGA CCCTGAGTTC GAGGCCATCC TGGACACTGT GGATCCTAAC 
AGGGACGGCC ACGTCTCCCT GCAAGAGTAC ATGGCCTTTA TGATCAGCCG TGAGACCGAG 
AACGTCAAGT CCAGCGAGGA GATTGAGAGC GCCTTCCGGG CCCTGAGCTC TGAAGGGAAG 
CCTTATGTGA CCAAGGAGGA GCTGTACCAG AACCTGACTC GGGAACAAGC CGACTACTGC 
GTCTCCCACA TGAAGCCCTA CGTGGACGGC AAGGGCCGCG AACTCCCCAC TGCTTTTGAC 
TACGTCGAGT TCACCCGCTC GCTCTTCGTG AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854806.1
CDS	4..7359
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLHQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMYRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVDHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWESLKSKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGAMPRDESDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKEKLDVLDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQSFADQLIAGGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDMGNSLIDRGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDAINGRFQKIKSMAASRRARLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLVKKNNHHEENISSKMKGLNGKVSDLEKAASQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHAALMKRWSQLLANSATRKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_024999038.1
Organism	Bos taurus(cattle)
Definition	Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999038.1

ORF Insert Sequence:

ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT 
GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT 
CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC 
AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG 
AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC 
CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG 
AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT 
GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC 
CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC 
CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC 
CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT 
AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT 
GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG 
GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC 
ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC 
TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG 
AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG 
GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA 
GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG 
AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG 
CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG 
GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA 
ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG 
AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT 
GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT 
TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG 
CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT 
GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG 
AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG 
CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT 
CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC 
GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA 
GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG 
GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT 
CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG 
AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC 
AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC 
GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC 
CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC 
GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA 
GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC 
GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC 
AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC 
GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC 
AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGTACC ATTCTCTGCT GGAACTGGGA 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG 
AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC 
GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC 
AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA 
GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC 
AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG 
CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA 
GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC 
TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG 
GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC 
CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA 
TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC 
CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT 
CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG 
TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC 
GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG 
ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG 
GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG 
ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC 
GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG 
ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA 
GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG 
GACCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ATCAGAAGCA CCAGGCCTTC 
GAGGCAGAAC TGCACGCCAA TGCGGACCGG ATCCGTGGGG TCATCGACAT GGGCAACTCC 
CTCATTGACC GCGGGGCCTG CGCCGGCAGC GAGGATGCCG TCAAGGCCCG GCTGGCCGCC 
TTAGCTGACC AGTGGCAGTT CCTGGTGCAG AAGTCGGCTG AGAAGAGCCA GAAGCTGAAA 
GAAGCCAACA AGCAGCAGAA CTTCAACACT GGCATCAAGG ACTTCGACTT CTGGCTCTCT 
GAGGTGGAAG CCCTGCTGGC CTCTGAGGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC 
TTGCTAAAAA AACATCAGCT GCTGGAGGCA GATATCTCTG CCCACGAGGA CCGCCTGAAG 
GACCTCAACA GCCAGGCGGA CAGCCTGATG ACCAGCAGCG CCTTCGACAC CTCCCAGGTG 
AAGGACAAGC GGGACGCCAT CAACGGCCGT TTCCAGAAGA TCAAGAGCAT GGCCGCCTCG 
AGGCGAGCTA GGCTGAATGA GTCCCACCGC CTGCACCAGT TTTTCCGGGA CATGGATGAT 
GAGGAGTCCT GGATCAAGGA AAAGAAGCTG CTGGTGAGCT CAGAGGACTA CGGCCGTGAC 
CTGACGGGCG TCCAGAACCT GAGGAAGAAG CACAAACGGC TGGAAGCAGA GCTGGCTGCC 
CACGAGCCGG CCATTCAGGG TGTCCTGGAC ACTGGAAAGA AGCTGTCTGA TGACAACACC 
ATCGGGAAGG AGGAGATTCA GCAGCGCCTG GCGCAGTTCG TGGAGCACTG GAAGGAGCTG 
AAGCAGCTGG CAGCTGCCCG GGGCCAGCGG CTAGAAGAAT CTTTGGAATA TCAGCAGTTT 
GTAGCCAACG TGGAGGAGGA GGAAGCCTGG ATCAATGAGA AGATGACCCT GGTGGCCAGT 
GAAGATTATG GAGACACTCT TGCTGCCATC CAGGGCTTGC TGAAGAAACA CGAAGCCTTC 
GAGACGGACT TCACTGTCCA CAAGGACCGA GTGAATGATG TCTGCACCAA CGGCCAGGAC 
CTGGTTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CCAAGATGAA GGGCTTGAAC 
GGGAAAGTGT CAGACTTGGA GAAGGCTGCC TCCCAGAGGA AGGCCAAGCT CGACGAGAAC 
TCGGCCTTCC TCCAGTTCAA CTGGAAAGCC GATGTGGTGG AGTCCTGGAT CGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGT CGAGATCTGT CTTCTGTCCA AACTCTCCTC 
ACCAAACAGG AAACCTTCGA TGCTGGCCTG CAGGCCTTCC AGCAGGAGGG CATCGCCAAC 
ATCACTGCCC TCAAAGATCA GCTGCTGGCC GCCAAGCACG TTCAGTCCAA GGCCATCGAG 
GCCCGCCATG CTGCCCTCAT GAAGAGGTGG AGCCAGCTCC TGGCCAACTC GGCCACCCGG 
AAGAAGAAGC TGCTGGAGGC ACAGAGTCAC TTCCGCAAGG TGGAGGATCT CTTCCTGACC 
TTTGCTAAAA AGGCTTCAGC CTTCAACAGC TGGTTTGAAA ACGCAGAGGA GGACTTAACC 
GACCCAGTGC GCTGCAACTC TCTGGAAGAG ATCAAGGCCT TGAGAGAGGC CCACGACGCC 
TTCCGCTCCT CGTTGAGCTC GGCCCAGGCC GACTTCAACC AGCTGGCAGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT GGCCTCCAAC CCCTACACCT GGTTCACCAT GGAGGCCCTG 
GAGGAGACCT GGAGAAACCT ACAGAAGATC ATCAAGGAAA GGGAGCTGGA GCTGCAGAAG 
GAGCAGCGAC GGCAGGAGGA GAATGACAAG CTGCGCCAGG AGTTCGCCCA GCATGCCAAC 
GCCTTCCACC AGTGGATCCA GGAGACCAGG TCCTGCATGG TGGAAGAATC CGGGACCCTC 
GAATCACAGC TTGAAGCTAC CAAACGCAAA CACCAGGAGA TTCGAGCTAT GAGAAGTCAG 
CTCAAGAAGA TTGAAGACCT GGGGGCGGCC ATGGAGGAAG CGCTGATCCT GGACAACAAG 
TACACGGAGC ACAGCACCGT GGGCCTGGCC CAGCAGTGGG ACCAGCTCGA CCAGTTGGGC 
ATGCGCATGC AGCACAACCT GGAGCAGCAG ATCCAGGCCA GGAACACGAC CGGAGTGACC 
GAGGAGGCCC TCAAAGAATT CAGCATGATG TTCAAACACT TCGACAAGGA CAAGTCTGGC 
CGGCTGAACC ACCAGGAGTT CAAATCCTGT CTGCGCTCCC TGGGCTATGA CCTGCCCATG 
GTGGAGGAAG GGGAGCCCGA CCCTGAGTTC GAGGCCATCC TGGACACTGT GGATCCTAAC 
AGGGACGGCC ACGTCTCCCT GCAAGAGTAC ATGGCCTTTA TGATCAGCCG TGAGACCGAG 
AACGTCAAGT CCAGCGAGGA GATTGAGAGC GCCTTCCGGG CCCTGAGCTC TGAAGGGAAG 
CCTTATGTGA CCAAGGAGGA GCTGTACCAG AACCTGACTC GGGAACAAGC CGACTACTGC 
GTCTCCCACA TGAAGCCCTA CGTGGACGGC AAGGGCCGCG AACTCCCCAC TGCTTTTGAC 
TACGTCGAGT TCACCCGCTC GCTCTTCGTG AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_024999039.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854807.1 Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OBa220572
Clone ID Related Accession (Same CDS sequence)	XM_024999039.1
Accession Version	XM_024999039.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7353bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT 
GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT 
CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC 
AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG 
AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC 
CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG 
AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT 
GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC 
CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC 
CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC 
CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT 
AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT 
GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG 
GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC 
ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC 
TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG 
AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG 
GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA 
GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG 
AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG 
CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG 
GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA 
ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG 
AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT 
GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT 
TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG 
CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT 
GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG 
AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG 
CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT 
CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC 
GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA 
GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG 
GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT 
CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG 
AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC 
AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC 
GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC 
CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC 
GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA 
GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC 
GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC 
AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC 
GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC 
AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGACGC GCGTAACTAA GGAGGCTGGC 
AGCGTGTCTC TGCGTATGAA ACAGGTGGAA GAACTGTACC ATTCTCTGCT GGAACTGGGA 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG 
AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC 
GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC 
AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA 
GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC 
AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGG AGCTGAACGA GCGCTGGCGG 
TCCCTGCAGC AGCTGGCTGA GGAGCGGAGC CAACTCTTGG GCAGTGCCCA TGAAGTGCAG 
CGGTTCCACA GAGATGCTGA CGAAACCAAA GAGTGGATCG AAGAGAAGAA CCAGGCTCTA 
AACACAGACA ACTACGGCCA CGACCTGGCC AGTGTCCAGG CCCTGCAGCG CAAGCACGAG 
GGCTTCGAGA GGGACCTCGC AGCCCTTGGT GACAAGGTGA ACTCGCTCGG GGAGACAGCC 
GAGCGCCTGA TCCAGTCCCA CCCCGAGTCG GCAGAGGACC TGCAGGAGAA GTGCACTGAG 
CTGAACCAGG CATGGAGCAG CCTGGGCAAG CGTGCCGACC AGCGCAAGGC CAAGCTGGGC 
GACTCCCACG ACCTGCAGCG CTTCCTCAGC GACTTCCGGG ACCTCATGTC TTGGATCAAT 
GGAATCCGAG GTCTGGTGTC CTCAGACGAG CTAGCCAAGG ATGTCACCGG AGCTGAGGCC 
CTGCTGGAGC GACACCAGGA ACACCGGACA GAAATTGATG CCAGAGCTGG CACTTTCCAG 
GCATTCGAGC AGTTTGGGCA GCAGCTGTTG GCTCATGGGC ACTACGCCAG CCCCGAGATC 
AAGGAGAAGC TCGATGTTCT AGACCAGGAG CGTGCAGACC TTGAGAAGGC CTGGGTCCAG 
CGCAGGATGA TGCTGGATCA GTGCCTTGAA TTGCAGCTGT TCCATCGGGA TTGCGAGCAG 
GCCGAGAACT GGATGGCTGC CCGAGAGGCC TTTCTGAACA CAGAAGACAA AGGGGACTCG 
CTAGACAGTG TGGAGGCTCT GATCAAAAAA CACGAGGACT TTGATAAAGC AATTAATGTC 
CAGGAGGAGA AGATTGCTGC CCTGCAGTCC TTTGCTGACC AGCTCATCGC TGGTGGCCAC 
TACGCCAAAG GCGACATCTC CAGCCGGCGC AATGAGGTTC TGGACAGGTG GCGCCGTCTA 
AAAGCTCAGA TGATTGAGAA GAGGTCAAAG CTGGGAGAAT CACAGACCCT CCAGCAGTTC 
AGCCGGGATG TAGACGAGAT TGAAGCTTGG ATCAGTGAAA AATTACAGAC TGCAAGTGAT 
GAGTCATATA AGGACCCCAC CAACATCCAG AGCAAGCATC AGAAGCACCA GGCCTTCGAG 
GCAGAACTGC ACGCCAATGC GGACCGGATC CGTGGGGTCA TCGACATGGG CAACTCCCTC 
ATTGACCGCG GGGCCTGCGC CGGCAGCGAG GATGCCGTCA AGGCCCGGCT GGCCGCCTTA 
GCTGACCAGT GGCAGTTCCT GGTGCAGAAG TCGGCTGAGA AGAGCCAGAA GCTGAAAGAA 
GCCAACAAGC AGCAGAACTT CAACACTGGC ATCAAGGACT TCGACTTCTG GCTCTCTGAG 
GTGGAAGCCC TGCTGGCCTC TGAGGATTAT GGCAAAGACC TGGCTTCTGT GAACAACTTG 
CTAAAAAAAC ATCAGCTGCT GGAGGCAGAT ATCTCTGCCC ACGAGGACCG CCTGAAGGAC 
CTCAACAGCC AGGCGGACAG CCTGATGACC AGCAGCGCCT TCGACACCTC CCAGGTGAAG 
GACAAGCGGG ACGCCATCAA CGGCCGTTTC CAGAAGATCA AGAGCATGGC CGCCTCGAGG 
CGAGCTAGGC TGAATGAGTC CCACCGCCTG CACCAGTTTT TCCGGGACAT GGATGATGAG 
GAGTCCTGGA TCAAGGAAAA GAAGCTGCTG GTGAGCTCAG AGGACTACGG CCGTGACCTG 
ACGGGCGTCC AGAACCTGAG GAAGAAGCAC AAACGGCTGG AAGCAGAGCT GGCTGCCCAC 
GAGCCGGCCA TTCAGGGTGT CCTGGACACT GGAAAGAAGC TGTCTGATGA CAACACCATC 
GGGAAGGAGG AGATTCAGCA GCGCCTGGCG CAGTTCGTGG AGCACTGGAA GGAGCTGAAG 
CAGCTGGCAG CTGCCCGGGG CCAGCGGCTA GAAGAATCTT TGGAATATCA GCAGTTTGTA 
GCCAACGTGG AGGAGGAGGA AGCCTGGATC AATGAGAAGA TGACCCTGGT GGCCAGTGAA 
GATTATGGAG ACACTCTTGC TGCCATCCAG GGCTTGCTGA AGAAACACGA AGCCTTCGAG 
ACGGACTTCA CTGTCCACAA GGACCGAGTG AATGATGTCT GCACCAACGG CCAGGACCTG 
GTTAAGAAGA ACAATCACCA TGAGGAGAAC ATCTCTTCCA AGATGAAGGG CTTGAACGGG 
AAAGTGTCAG ACTTGGAGAA GGCTGCCTCC CAGAGGAAGG CCAAGCTCGA CGAGAACTCG 
GCCTTCCTCC AGTTCAACTG GAAAGCCGAT GTGGTGGAGT CCTGGATCGG TGAAAAGGAG 
AACAGCTTGA AGACAGATGA TTATGGTCGA GATCTGTCTT CTGTCCAAAC TCTCCTCACC 
AAACAGGAAA CCTTCGATGC TGGCCTGCAG GCCTTCCAGC AGGAGGGCAT CGCCAACATC 
ACTGCCCTCA AAGATCAGCT GCTGGCCGCC AAGCACGTTC AGTCCAAGGC CATCGAGGCC 
CGCCATGCTG CCCTCATGAA GAGGTGGAGC CAGCTCCTGG CCAACTCGGC CACCCGGAAG 
AAGAAGCTGC TGGAGGCACA GAGTCACTTC CGCAAGGTGG AGGATCTCTT CCTGACCTTT 
GCTAAAAAGG CTTCAGCCTT CAACAGCTGG TTTGAAAACG CAGAGGAGGA CTTAACCGAC 
CCAGTGCGCT GCAACTCTCT GGAAGAGATC AAGGCCTTGA GAGAGGCCCA CGACGCCTTC 
CGCTCCTCGT TGAGCTCGGC CCAGGCCGAC TTCAACCAGC TGGCAGAGCT GGACCGCCAG 
ATCAAGAGCT TCCGCGTGGC CTCCAACCCC TACACCTGGT TCACCATGGA GGCCCTGGAG 
GAGACCTGGA GAAACCTACA GAAGATCATC AAGGAAAGGG AGCTGGAGCT GCAGAAGGAG 
CAGCGACGGC AGGAGGAGAA TGACAAGCTG CGCCAGGAGT TCGCCCAGCA TGCCAACGCC 
TTCCACCAGT GGATCCAGGA GACCAGGTCC TGCATGGTGG AAGAATCCGG GACCCTCGAA 
TCACAGCTTG AAGCTACCAA ACGCAAACAC CAGGAGATTC GAGCTATGAG AAGTCAGCTC 
AAGAAGATTG AAGACCTGGG GGCGGCCATG GAGGAAGCGC TGATCCTGGA CAACAAGTAC 
ACGGAGCACA GCACCGTGGG CCTGGCCCAG CAGTGGGACC AGCTCGACCA GTTGGGCATG 
CGCATGCAGC ACAACCTGGA GCAGCAGATC CAGGCCAGGA ACACGACCGG AGTGACCGAG 
GAGGCCCTCA AAGAATTCAG CATGATGTTC AAACACTTCG ACAAGGACAA GTCTGGCCGG 
CTGAACCACC AGGAGTTCAA ATCCTGTCTG CGCTCCCTGG GCTATGACCT GCCCATGGTG 
GAGGAAGGGG AGCCCGACCC TGAGTTCGAG GCCATCCTGG ACACTGTGGA TCCTAACAGG 
GACGGCCACG TCTCCCTGCA AGAGTACATG GCCTTTATGA TCAGCCGTGA GACCGAGAAC 
GTCAAGTCCA GCGAGGAGAT TGAGAGCGCC TTCCGGGCCC TGAGCTCTGA AGGGAAGCCT 
TATGTGACCA AGGAGGAGCT GTACCAGAAC CTGACTCGGG AACAAGCCGA CTACTGCGTC 
TCCCACATGA AGCCCTACGT GGACGGCAAG GGCCGCGAAC TCCCCACTGC TTTTGACTAC 
GTCGAGTTCA CCCGCTCGCT CTTCGTGAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854807.1
CDS	4..7356
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLHQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMYRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVDHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWESLKSKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRVTKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGAMPRDESDSKTASPWKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKEKLDVLDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQSFADQLIAGGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQSKHQKHQAFEAELHANADRIRGVIDMGNSLIDRGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDAINGRFQKIKSMAASRRARLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLVKKNNHHEENISSKMKGLNGKVSDLEKAASQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHAALMKRWSQLLANSATRKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_024999039.1
Organism	Bos taurus(cattle)
Definition	Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999039.1

ORF Insert Sequence:

ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT 
GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT 
CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC 
AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG 
AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC 
CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG 
AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT 
GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC 
CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC 
CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC 
CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT 
AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT 
GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG 
GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC 
ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC 
TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG 
AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG 
GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA 
GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG 
AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG 
CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG 
GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA 
ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG 
AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT 
GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT 
TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG 
CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT 
GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG 
AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG 
CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT 
CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC 
GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA 
GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG 
GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT 
CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG 
AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC 
AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC 
GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC 
CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC 
GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA 
GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC 
GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC 
AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC 
GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC 
AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGACGC GCGTAACTAA GGAGGCTGGC 
AGCGTGTCTC TGCGTATGAA ACAGGTGGAA GAACTGTACC ATTCTCTGCT GGAACTGGGA 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG 
AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC 
GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC 
AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA 
GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC 
AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGG AGCTGAACGA GCGCTGGCGG 
TCCCTGCAGC AGCTGGCTGA GGAGCGGAGC CAACTCTTGG GCAGTGCCCA TGAAGTGCAG 
CGGTTCCACA GAGATGCTGA CGAAACCAAA GAGTGGATCG AAGAGAAGAA CCAGGCTCTA 
AACACAGACA ACTACGGCCA CGACCTGGCC AGTGTCCAGG CCCTGCAGCG CAAGCACGAG 
GGCTTCGAGA GGGACCTCGC AGCCCTTGGT GACAAGGTGA ACTCGCTCGG GGAGACAGCC 
GAGCGCCTGA TCCAGTCCCA CCCCGAGTCG GCAGAGGACC TGCAGGAGAA GTGCACTGAG 
CTGAACCAGG CATGGAGCAG CCTGGGCAAG CGTGCCGACC AGCGCAAGGC CAAGCTGGGC 
GACTCCCACG ACCTGCAGCG CTTCCTCAGC GACTTCCGGG ACCTCATGTC TTGGATCAAT 
GGAATCCGAG GTCTGGTGTC CTCAGACGAG CTAGCCAAGG ATGTCACCGG AGCTGAGGCC 
CTGCTGGAGC GACACCAGGA ACACCGGACA GAAATTGATG CCAGAGCTGG CACTTTCCAG 
GCATTCGAGC AGTTTGGGCA GCAGCTGTTG GCTCATGGGC ACTACGCCAG CCCCGAGATC 
AAGGAGAAGC TCGATGTTCT AGACCAGGAG CGTGCAGACC TTGAGAAGGC CTGGGTCCAG 
CGCAGGATGA TGCTGGATCA GTGCCTTGAA TTGCAGCTGT TCCATCGGGA TTGCGAGCAG 
GCCGAGAACT GGATGGCTGC CCGAGAGGCC TTTCTGAACA CAGAAGACAA AGGGGACTCG 
CTAGACAGTG TGGAGGCTCT GATCAAAAAA CACGAGGACT TTGATAAAGC AATTAATGTC 
CAGGAGGAGA AGATTGCTGC CCTGCAGTCC TTTGCTGACC AGCTCATCGC TGGTGGCCAC 
TACGCCAAAG GCGACATCTC CAGCCGGCGC AATGAGGTTC TGGACAGGTG GCGCCGTCTA 
AAAGCTCAGA TGATTGAGAA GAGGTCAAAG CTGGGAGAAT CACAGACCCT CCAGCAGTTC 
AGCCGGGATG TAGACGAGAT TGAAGCTTGG ATCAGTGAAA AATTACAGAC TGCAAGTGAT 
GAGTCATATA AGGACCCCAC CAACATCCAG AGCAAGCATC AGAAGCACCA GGCCTTCGAG 
GCAGAACTGC ACGCCAATGC GGACCGGATC CGTGGGGTCA TCGACATGGG CAACTCCCTC 
ATTGACCGCG GGGCCTGCGC CGGCAGCGAG GATGCCGTCA AGGCCCGGCT GGCCGCCTTA 
GCTGACCAGT GGCAGTTCCT GGTGCAGAAG TCGGCTGAGA AGAGCCAGAA GCTGAAAGAA 
GCCAACAAGC AGCAGAACTT CAACACTGGC ATCAAGGACT TCGACTTCTG GCTCTCTGAG 
GTGGAAGCCC TGCTGGCCTC TGAGGATTAT GGCAAAGACC TGGCTTCTGT GAACAACTTG 
CTAAAAAAAC ATCAGCTGCT GGAGGCAGAT ATCTCTGCCC ACGAGGACCG CCTGAAGGAC 
CTCAACAGCC AGGCGGACAG CCTGATGACC AGCAGCGCCT TCGACACCTC CCAGGTGAAG 
GACAAGCGGG ACGCCATCAA CGGCCGTTTC CAGAAGATCA AGAGCATGGC CGCCTCGAGG 
CGAGCTAGGC TGAATGAGTC CCACCGCCTG CACCAGTTTT TCCGGGACAT GGATGATGAG 
GAGTCCTGGA TCAAGGAAAA GAAGCTGCTG GTGAGCTCAG AGGACTACGG CCGTGACCTG 
ACGGGCGTCC AGAACCTGAG GAAGAAGCAC AAACGGCTGG AAGCAGAGCT GGCTGCCCAC 
GAGCCGGCCA TTCAGGGTGT CCTGGACACT GGAAAGAAGC TGTCTGATGA CAACACCATC 
GGGAAGGAGG AGATTCAGCA GCGCCTGGCG CAGTTCGTGG AGCACTGGAA GGAGCTGAAG 
CAGCTGGCAG CTGCCCGGGG CCAGCGGCTA GAAGAATCTT TGGAATATCA GCAGTTTGTA 
GCCAACGTGG AGGAGGAGGA AGCCTGGATC AATGAGAAGA TGACCCTGGT GGCCAGTGAA 
GATTATGGAG ACACTCTTGC TGCCATCCAG GGCTTGCTGA AGAAACACGA AGCCTTCGAG 
ACGGACTTCA CTGTCCACAA GGACCGAGTG AATGATGTCT GCACCAACGG CCAGGACCTG 
GTTAAGAAGA ACAATCACCA TGAGGAGAAC ATCTCTTCCA AGATGAAGGG CTTGAACGGG 
AAAGTGTCAG ACTTGGAGAA GGCTGCCTCC CAGAGGAAGG CCAAGCTCGA CGAGAACTCG 
GCCTTCCTCC AGTTCAACTG GAAAGCCGAT GTGGTGGAGT CCTGGATCGG TGAAAAGGAG 
AACAGCTTGA AGACAGATGA TTATGGTCGA GATCTGTCTT CTGTCCAAAC TCTCCTCACC 
AAACAGGAAA CCTTCGATGC TGGCCTGCAG GCCTTCCAGC AGGAGGGCAT CGCCAACATC 
ACTGCCCTCA AAGATCAGCT GCTGGCCGCC AAGCACGTTC AGTCCAAGGC CATCGAGGCC 
CGCCATGCTG CCCTCATGAA GAGGTGGAGC CAGCTCCTGG CCAACTCGGC CACCCGGAAG 
AAGAAGCTGC TGGAGGCACA GAGTCACTTC CGCAAGGTGG AGGATCTCTT CCTGACCTTT 
GCTAAAAAGG CTTCAGCCTT CAACAGCTGG TTTGAAAACG CAGAGGAGGA CTTAACCGAC 
CCAGTGCGCT GCAACTCTCT GGAAGAGATC AAGGCCTTGA GAGAGGCCCA CGACGCCTTC 
CGCTCCTCGT TGAGCTCGGC CCAGGCCGAC TTCAACCAGC TGGCAGAGCT GGACCGCCAG 
ATCAAGAGCT TCCGCGTGGC CTCCAACCCC TACACCTGGT TCACCATGGA GGCCCTGGAG 
GAGACCTGGA GAAACCTACA GAAGATCATC AAGGAAAGGG AGCTGGAGCT GCAGAAGGAG 
CAGCGACGGC AGGAGGAGAA TGACAAGCTG CGCCAGGAGT TCGCCCAGCA TGCCAACGCC 
TTCCACCAGT GGATCCAGGA GACCAGGTCC TGCATGGTGG AAGAATCCGG GACCCTCGAA 
TCACAGCTTG AAGCTACCAA ACGCAAACAC CAGGAGATTC GAGCTATGAG AAGTCAGCTC 
AAGAAGATTG AAGACCTGGG GGCGGCCATG GAGGAAGCGC TGATCCTGGA CAACAAGTAC 
ACGGAGCACA GCACCGTGGG CCTGGCCCAG CAGTGGGACC AGCTCGACCA GTTGGGCATG 
CGCATGCAGC ACAACCTGGA GCAGCAGATC CAGGCCAGGA ACACGACCGG AGTGACCGAG 
GAGGCCCTCA AAGAATTCAG CATGATGTTC AAACACTTCG ACAAGGACAA GTCTGGCCGG 
CTGAACCACC AGGAGTTCAA ATCCTGTCTG CGCTCCCTGG GCTATGACCT GCCCATGGTG 
GAGGAAGGGG AGCCCGACCC TGAGTTCGAG GCCATCCTGG ACACTGTGGA TCCTAACAGG 
GACGGCCACG TCTCCCTGCA AGAGTACATG GCCTTTATGA TCAGCCGTGA GACCGAGAAC 
GTCAAGTCCA GCGAGGAGAT TGAGAGCGCC TTCCGGGCCC TGAGCTCTGA AGGGAAGCCT 
TATGTGACCA AGGAGGAGCT GTACCAGAAC CTGACTCGGG AACAAGCCGA CTACTGCGTC 
TCCCACATGA AGCCCTACGT GGACGGCAAG GGCCGCGAAC TCCCCACTGC TTTTGACTAC 
GTCGAGTTCA CCCGCTCGCT CTTCGTGAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_024999041.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854809.1 Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OBa220573
Clone ID Related Accession (Same CDS sequence)	XM_024999041.1
Accession Version	XM_024999041.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT 
GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT 
CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC 
AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG 
AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC 
CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG 
AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT 
GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC 
CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC 
CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC 
CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT 
AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT 
GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG 
GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC 
ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC 
TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG 
AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG 
GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA 
GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG 
AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG 
CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG 
GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA 
ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG 
AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT 
GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT 
TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG 
CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT 
GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG 
AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG 
CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT 
CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC 
GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA 
GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG 
GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT 
CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG 
AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC 
AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC 
GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC 
CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC 
GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA 
GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC 
GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC 
AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC 
GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC 
AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGACGC GCGTAACTAA GGAGGCTGGC 
AGCGTGTCTC TGCGTATGAA ACAGGTGGAA GAACTGTACC ATTCTCTGCT GGAACTGGGA 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG 
AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC 
GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC 
AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA 
GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC 
AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGG AGCTGAACGA GCGCTGGCGG 
TCCCTGCAGC AGCTGGCTGA GGAGCGGAGC CAACTCTTGG GCAGTGCCCA TGAAGTGCAG 
CGGTTCCACA GAGATGCTGA CGAAACCAAA GAGTGGATCG AAGAGAAGAA CCAGGCTCTA 
AACACAGACA ACTACGGCCA CGACCTGGCC AGTGTCCAGG CCCTGCAGCG CAAGCACGAG 
GGCTTCGAGA GGGACCTCGC AGCCCTTGGT GACAAGGTGA ACTCGCTCGG GGAGACAGCC 
GAGCGCCTGA TCCAGTCCCA CCCCGAGTCG GCAGAGGACC TGCAGGAGAA GTGCACTGAG 
CTGAACCAGG CATGGAGCAG CCTGGGCAAG CGTGCCGACC AGCGCAAGGC CAAGCTGGGC 
GACTCCCACG ACCTGCAGCG CTTCCTCAGC GACTTCCGGG ACCTCATGTC TTGGATCAAT 
GGAATCCGAG GTCTGGTGTC CTCAGACGAG CTAGCCAAGG ATGTCACCGG AGCTGAGGCC 
CTGCTGGAGC GACACCAGGA ACACCGGACA GAAATTGATG CCAGAGCTGG CACTTTCCAG 
GCATTCGAGC AGTTTGGGCA GCAGCTGTTG GCTCATGGGC ACTACGCCAG CCCCGAGATC 
AAGGAGAAGC TCGATGTTCT AGACCAGGAG CGTGCAGACC TTGAGAAGGC CTGGGTCCAG 
CGCAGGATGA TGCTGGATCA GTGCCTTGAA TTGCAGCTGT TCCATCGGGA TTGCGAGCAG 
GCCGAGAACT GGATGGCTGC CCGAGAGGCC TTTCTGAACA CAGAAGACAA AGGGGACTCG 
CTAGACAGTG TGGAGGCTCT GATCAAAAAA CACGAGGACT TTGATAAAGC AATTAATGTC 
CAGGAGGAGA AGATTGCTGC CCTGCAGTCC TTTGCTGACC AGCTCATCGC TGGTGGCCAC 
TACGCCAAAG GCGACATCTC CAGCCGGCGC AATGAGGTTC TGGACAGGTG GCGCCGTCTA 
AAAGCTCAGA TGATTGAGAA GAGGTCAAAG CTGGGAGAAT CACAGACCCT CCAGCAGTTC 
AGCCGGGATG TAGACGAGAT TGAAGCTTGG ATCAGTGAAA AATTACAGAC TGCAAGTGAT 
GAGTCATATA AGGACCCCAC CAACATCCAG AGCAAGCATC AGAAGCACCA GGCCTTCGAG 
GCAGAACTGC ACGCCAATGC GGACCGGATC CGTGGGGTCA TCGACATGGG CAACTCCCTC 
ATTGACCGCG GGGCCTGCGC CGGCAGCGAG GATGCCGTCA AGGCCCGGCT GGCCGCCTTA 
GCTGACCAGT GGCAGTTCCT GGTGCAGAAG TCGGCTGAGA AGAGCCAGAA GCTGAAAGAA 
GCCAACAAGC AGCAGAACTT CAACACTGGC ATCAAGGACT TCGACTTCTG GCTCTCTGAG 
GTGGAAGCCC TGCTGGCCTC TGAGGATTAT GGCAAAGACC TGGCTTCTGT GAACAACTTG 
CTAAAAAAAC ATCAGCTGCT GGAGGCAGAT ATCTCTGCCC ACGAGGACCG CCTGAAGGAC 
CTCAACAGCC AGGCGGACAG CCTGATGACC AGCAGCGCCT TCGACACCTC CCAGGTGAAG 
GACAAGCGGG ACGCCATCAA CGGCCGTTTC CAGAAGATCA AGAGCATGGC CGCCTCGAGG 
CGAGCTAGGC TGAATGAGTC CCACCGCCTG CACCAGTTTT TCCGGGACAT GGATGATGAG 
GAGTCCTGGA TCAAGGAAAA GAAGCTGCTG GTGAGCTCAG AGGACTACGG CCGTGACCTG 
ACGGGCGTCC AGAACCTGAG GAAGAAGCAC AAACGGCTGG AAGCAGAGCT GGCTGCCCAC 
GAGCCGGCCA TTCAGGGTGT CCTGGACACT GGAAAGAAGC TGTCTGATGA CAACACCATC 
GGGAAGGAGG AGATTCAGCA GCGCCTGGCG CAGTTCGTGG AGCACTGGAA GGAGCTGAAG 
CAGCTGGCAG CTGCCCGGGG CCAGCGGCTA GAAGAATCTT TGGAATATCA GCAGTTTGTA 
GCCAACGTGG AGGAGGAGGA AGCCTGGATC AATGAGAAGA TGACCCTGGT GGCCAGTGAA 
GATTATGGAG ACACTCTTGC TGCCATCCAG GGCTTGCTGA AGAAACACGA AGCCTTCGAG 
ACGGACTTCA CTGTCCACAA GGACCGAGTG AATGATGTCT GCACCAACGG CCAGGACCTG 
GTTAAGAAGA ACAATCACCA TGAGGAGAAC ATCTCTTCCA AGATGAAGGG CTTGAACGGG 
AAAGTGTCAG ACTTGGAGAA GGCTGCCTCC CAGAGGAAGG CCAAGCTCGA CGAGAACTCG 
GCCTTCCTCC AGTTCAACTG GAAAGCCGAT GTGGTGGAGT CCTGGATCGG TGAAAAGGAG 
AACAGCTTGA AGACAGATGA TTATGGTCGA GATCTGTCTT CTGTCCAAAC TCTCCTCACC 
AAACAGGAAA CCTTCGATGC TGGCCTGCAG GCCTTCCAGC AGGAGGGCAT CGCCAACATC 
ACTGCCCTCA AAGATCAGCT GCTGGCCGCC AAGCACGTTC AGTCCAAGGC CATCGAGGCC 
CGCCATGCTG CCCTCATGAA GAGGTGGAGC CAGCTCCTGG CCAACTCGGC CACCCGGAAG 
AAGAAGCTGC TGGAGGCACA GAGTCACTTC CGCAAGGTGG AGGATCTCTT CCTGACCTTT 
GCTAAAAAGG CTTCAGCCTT CAACAGCTGG TTTGAAAACG CAGAGGAGGA CTTAACCGAC 
CCAGTGCGCT GCAACTCTCT GGAAGAGATC AAGGCCTTGA GAGAGGCCCA CGACGCCTTC 
CGCTCCTCGT TGAGCTCGGC CCAGGCCGAC TTCAACCAGC TGGCAGAGCT GGACCGCCAG 
ATCAAGAGCT TCCGCGTGGC CTCCAACCCC TACACCTGGT TCACCATGGA GGCCCTGGAG 
GAGACCTGGA GAAACCTACA GAAGATCATC AAGGAAAGGG AGCTGGAGCT GCAGAAGGAG 
CAGCGACGGC AGGAGGAGAA TGACAAGCTG CGCCAGGAGT TCGCCCAGCA TGCCAACGCC 
TTCCACCAGT GGATCCAGGA GACCAGAACG TACCTCCTCG ATGGGTCCTG CATGGTGGAA 
GAATCCGGGA CCCTCGAATC ACAGCTTGAA GCTACCAAAC GCAAACACCA GGAGATTCGA 
GCTATGAGAA GTCAGCTCAA GAAGATTGAA GACCTGGGGG CGGCCATGGA GGAAGCGCTG 
ATCCTGGACA ACAAGTACAC GGAGCACAGC ACCGTGGGCC TGGCCCAGCA GTGGGACCAG 
CTCGACCAGT TGGGCATGCG CATGCAGCAC AACCTGGAGC AGCAGATCCA GGCCAGGAAC 
ACGACCGGAG TGACCGAGGA GGCCCTCAAA GAATTCAGCA TGATGTTCAA ACACTTCGAC 
AAGGACAAGT CTGGCCGGCT GAACCACCAG GAGTTCAAAT CCTGTCTGCG CTCCCTGGGC 
TATGACCTGC CCATGGTGGA GGAAGGGGAG CCCGACCCTG AGTTCGAGGC CATCCTGGAC 
ACTGTGGATC CTAACAGGGA CGGCCACGTC TCCCTGCAAG AGTACATGGC CTTTATGATC 
AGCCGTGAGA CCGAGAACGT CAAGTCCAGC GAGGAGATTG AGAGCGCCTT CCGGGCCCTG 
AGCTCTGAAG GGAAGCCTTA TGTGACCAAG GAGGAGCTGT ACCAGAACCT GACTCGGGAA 
CAAGCCGACT ACTGCGTCTC CCACATGAAG CCCTACGTGG ACGGCAAGGG CCGCGAACTC 
CCCACTGCTT TTGACTACGT CGAGTTCACC CGCTCGCTCT TCGTGAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854809.1
CDS	120..7490
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLHQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMYRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVDHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWESLKSKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRVTKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGAMPRDESDSKTASPWKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKEKLDVLDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQSFADQLIAGGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQSKHQKHQAFEAELHANADRIRGVIDMGNSLIDRGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDAINGRFQKIKSMAASRRARLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLVKKNNHHEENISSKMKGLNGKVSDLEKAASQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHAALMKRWSQLLANSATRKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_024999041.1
Organism	Bos taurus(cattle)
Definition	Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999041.1

ORF Insert Sequence:

ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT 
GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT 
CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC 
AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG 
AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC 
CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG 
AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT 
GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC 
CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC 
CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC 
CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT 
AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT 
GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG 
GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC 
ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC 
TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG 
AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG 
GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA 
GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG 
AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG 
CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG 
GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA 
ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG 
AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT 
GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT 
TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG 
CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT 
GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG 
AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG 
CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT 
CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC 
GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA 
GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG 
GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT 
CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG 
AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC 
AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC 
GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC 
CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC 
GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA 
GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC 
GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC 
AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC 
GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC 
AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGACGC GCGTAACTAA GGAGGCTGGC 
AGCGTGTCTC TGCGTATGAA ACAGGTGGAA GAACTGTACC ATTCTCTGCT GGAACTGGGA 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG 
AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC 
GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC 
AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA 
GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC 
AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGG AGCTGAACGA GCGCTGGCGG 
TCCCTGCAGC AGCTGGCTGA GGAGCGGAGC CAACTCTTGG GCAGTGCCCA TGAAGTGCAG 
CGGTTCCACA GAGATGCTGA CGAAACCAAA GAGTGGATCG AAGAGAAGAA CCAGGCTCTA 
AACACAGACA ACTACGGCCA CGACCTGGCC AGTGTCCAGG CCCTGCAGCG CAAGCACGAG 
GGCTTCGAGA GGGACCTCGC AGCCCTTGGT GACAAGGTGA ACTCGCTCGG GGAGACAGCC 
GAGCGCCTGA TCCAGTCCCA CCCCGAGTCG GCAGAGGACC TGCAGGAGAA GTGCACTGAG 
CTGAACCAGG CATGGAGCAG CCTGGGCAAG CGTGCCGACC AGCGCAAGGC CAAGCTGGGC 
GACTCCCACG ACCTGCAGCG CTTCCTCAGC GACTTCCGGG ACCTCATGTC TTGGATCAAT 
GGAATCCGAG GTCTGGTGTC CTCAGACGAG CTAGCCAAGG ATGTCACCGG AGCTGAGGCC 
CTGCTGGAGC GACACCAGGA ACACCGGACA GAAATTGATG CCAGAGCTGG CACTTTCCAG 
GCATTCGAGC AGTTTGGGCA GCAGCTGTTG GCTCATGGGC ACTACGCCAG CCCCGAGATC 
AAGGAGAAGC TCGATGTTCT AGACCAGGAG CGTGCAGACC TTGAGAAGGC CTGGGTCCAG 
CGCAGGATGA TGCTGGATCA GTGCCTTGAA TTGCAGCTGT TCCATCGGGA TTGCGAGCAG 
GCCGAGAACT GGATGGCTGC CCGAGAGGCC TTTCTGAACA CAGAAGACAA AGGGGACTCG 
CTAGACAGTG TGGAGGCTCT GATCAAAAAA CACGAGGACT TTGATAAAGC AATTAATGTC 
CAGGAGGAGA AGATTGCTGC CCTGCAGTCC TTTGCTGACC AGCTCATCGC TGGTGGCCAC 
TACGCCAAAG GCGACATCTC CAGCCGGCGC AATGAGGTTC TGGACAGGTG GCGCCGTCTA 
AAAGCTCAGA TGATTGAGAA GAGGTCAAAG CTGGGAGAAT CACAGACCCT CCAGCAGTTC 
AGCCGGGATG TAGACGAGAT TGAAGCTTGG ATCAGTGAAA AATTACAGAC TGCAAGTGAT 
GAGTCATATA AGGACCCCAC CAACATCCAG AGCAAGCATC AGAAGCACCA GGCCTTCGAG 
GCAGAACTGC ACGCCAATGC GGACCGGATC CGTGGGGTCA TCGACATGGG CAACTCCCTC 
ATTGACCGCG GGGCCTGCGC CGGCAGCGAG GATGCCGTCA AGGCCCGGCT GGCCGCCTTA 
GCTGACCAGT GGCAGTTCCT GGTGCAGAAG TCGGCTGAGA AGAGCCAGAA GCTGAAAGAA 
GCCAACAAGC AGCAGAACTT CAACACTGGC ATCAAGGACT TCGACTTCTG GCTCTCTGAG 
GTGGAAGCCC TGCTGGCCTC TGAGGATTAT GGCAAAGACC TGGCTTCTGT GAACAACTTG 
CTAAAAAAAC ATCAGCTGCT GGAGGCAGAT ATCTCTGCCC ACGAGGACCG CCTGAAGGAC 
CTCAACAGCC AGGCGGACAG CCTGATGACC AGCAGCGCCT TCGACACCTC CCAGGTGAAG 
GACAAGCGGG ACGCCATCAA CGGCCGTTTC CAGAAGATCA AGAGCATGGC CGCCTCGAGG 
CGAGCTAGGC TGAATGAGTC CCACCGCCTG CACCAGTTTT TCCGGGACAT GGATGATGAG 
GAGTCCTGGA TCAAGGAAAA GAAGCTGCTG GTGAGCTCAG AGGACTACGG CCGTGACCTG 
ACGGGCGTCC AGAACCTGAG GAAGAAGCAC AAACGGCTGG AAGCAGAGCT GGCTGCCCAC 
GAGCCGGCCA TTCAGGGTGT CCTGGACACT GGAAAGAAGC TGTCTGATGA CAACACCATC 
GGGAAGGAGG AGATTCAGCA GCGCCTGGCG CAGTTCGTGG AGCACTGGAA GGAGCTGAAG 
CAGCTGGCAG CTGCCCGGGG CCAGCGGCTA GAAGAATCTT TGGAATATCA GCAGTTTGTA 
GCCAACGTGG AGGAGGAGGA AGCCTGGATC AATGAGAAGA TGACCCTGGT GGCCAGTGAA 
GATTATGGAG ACACTCTTGC TGCCATCCAG GGCTTGCTGA AGAAACACGA AGCCTTCGAG 
ACGGACTTCA CTGTCCACAA GGACCGAGTG AATGATGTCT GCACCAACGG CCAGGACCTG 
GTTAAGAAGA ACAATCACCA TGAGGAGAAC ATCTCTTCCA AGATGAAGGG CTTGAACGGG 
AAAGTGTCAG ACTTGGAGAA GGCTGCCTCC CAGAGGAAGG CCAAGCTCGA CGAGAACTCG 
GCCTTCCTCC AGTTCAACTG GAAAGCCGAT GTGGTGGAGT CCTGGATCGG TGAAAAGGAG 
AACAGCTTGA AGACAGATGA TTATGGTCGA GATCTGTCTT CTGTCCAAAC TCTCCTCACC 
AAACAGGAAA CCTTCGATGC TGGCCTGCAG GCCTTCCAGC AGGAGGGCAT CGCCAACATC 
ACTGCCCTCA AAGATCAGCT GCTGGCCGCC AAGCACGTTC AGTCCAAGGC CATCGAGGCC 
CGCCATGCTG CCCTCATGAA GAGGTGGAGC CAGCTCCTGG CCAACTCGGC CACCCGGAAG 
AAGAAGCTGC TGGAGGCACA GAGTCACTTC CGCAAGGTGG AGGATCTCTT CCTGACCTTT 
GCTAAAAAGG CTTCAGCCTT CAACAGCTGG TTTGAAAACG CAGAGGAGGA CTTAACCGAC 
CCAGTGCGCT GCAACTCTCT GGAAGAGATC AAGGCCTTGA GAGAGGCCCA CGACGCCTTC 
CGCTCCTCGT TGAGCTCGGC CCAGGCCGAC TTCAACCAGC TGGCAGAGCT GGACCGCCAG 
ATCAAGAGCT TCCGCGTGGC CTCCAACCCC TACACCTGGT TCACCATGGA GGCCCTGGAG 
GAGACCTGGA GAAACCTACA GAAGATCATC AAGGAAAGGG AGCTGGAGCT GCAGAAGGAG 
CAGCGACGGC AGGAGGAGAA TGACAAGCTG CGCCAGGAGT TCGCCCAGCA TGCCAACGCC 
TTCCACCAGT GGATCCAGGA GACCAGAACG TACCTCCTCG ATGGGTCCTG CATGGTGGAA 
GAATCCGGGA CCCTCGAATC ACAGCTTGAA GCTACCAAAC GCAAACACCA GGAGATTCGA 
GCTATGAGAA GTCAGCTCAA GAAGATTGAA GACCTGGGGG CGGCCATGGA GGAAGCGCTG 
ATCCTGGACA ACAAGTACAC GGAGCACAGC ACCGTGGGCC TGGCCCAGCA GTGGGACCAG 
CTCGACCAGT TGGGCATGCG CATGCAGCAC AACCTGGAGC AGCAGATCCA GGCCAGGAAC 
ACGACCGGAG TGACCGAGGA GGCCCTCAAA GAATTCAGCA TGATGTTCAA ACACTTCGAC 
AAGGACAAGT CTGGCCGGCT GAACCACCAG GAGTTCAAAT CCTGTCTGCG CTCCCTGGGC 
TATGACCTGC CCATGGTGGA GGAAGGGGAG CCCGACCCTG AGTTCGAGGC CATCCTGGAC 
ACTGTGGATC CTAACAGGGA CGGCCACGTC TCCCTGCAAG AGTACATGGC CTTTATGATC 
AGCCGTGAGA CCGAGAACGT CAAGTCCAGC GAGGAGATTG AGAGCGCCTT CCGGGCCCTG 
AGCTCTGAAG GGAAGCCTTA TGTGACCAAG GAGGAGCTGT ACCAGAACCT GACTCGGGAA 
CAAGCCGACT ACTGCGTCTC CCACATGAAG CCCTACGTGG ACGGCAAGGG CCGCGAACTC 
CCCACTGCTT TTGACTACGT CGAGTTCACC CGCTCGCTCT TCGTGAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_024999043.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854811.1 Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X17, mRNA.

Price & Availability↑

CloneID	OBa220574
Clone ID Related Accession (Same CDS sequence)	XM_024999043.1
Accession Version	XM_024999043.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7341bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT 
GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT 
CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC 
AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG 
AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC 
CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG 
AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT 
GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC 
CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC 
CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC 
CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT 
AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT 
GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG 
GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC 
ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC 
TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG 
AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG 
GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA 
GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG 
AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG 
CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG 
GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA 
ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG 
AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT 
GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT 
TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG 
CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT 
GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG 
AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG 
CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT 
CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC 
GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA 
GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG 
GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT 
CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG 
AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC 
AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC 
GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC 
CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC 
GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA 
GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC 
GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC 
AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC 
GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC 
AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGTACC ATTCTCTGCT GGAACTGGGA 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG 
AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC 
GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC 
AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA 
GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC 
AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG 
CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA 
GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC 
TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG 
GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC 
CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA 
TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC 
CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT 
CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG 
TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC 
GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG 
ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG 
GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG 
ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC 
GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG 
ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA 
GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG 
GACCCCACCA ACATCCAGAG CAAGCATCAG AAGCACCAGG CCTTCGAGGC AGAACTGCAC 
GCCAATGCGG ACCGGATCCG TGGGGTCATC GACATGGGCA ACTCCCTCAT TGACCGCGGG 
GCCTGCGCCG GCAGCGAGGA TGCCGTCAAG GCCCGGCTGG CCGCCTTAGC TGACCAGTGG 
CAGTTCCTGG TGCAGAAGTC GGCTGAGAAG AGCCAGAAGC TGAAAGAAGC CAACAAGCAG 
CAGAACTTCA ACACTGGCAT CAAGGACTTC GACTTCTGGC TCTCTGAGGT GGAAGCCCTG 
CTGGCCTCTG AGGATTATGG CAAAGACCTG GCTTCTGTGA ACAACTTGCT AAAAAAACAT 
CAGCTGCTGG AGGCAGATAT CTCTGCCCAC GAGGACCGCC TGAAGGACCT CAACAGCCAG 
GCGGACAGCC TGATGACCAG CAGCGCCTTC GACACCTCCC AGGTGAAGGA CAAGCGGGAC 
GCCATCAACG GCCGTTTCCA GAAGATCAAG AGCATGGCCG CCTCGAGGCG AGCTAGGCTG 
AATGAGTCCC ACCGCCTGCA CCAGTTTTTC CGGGACATGG ATGATGAGGA GTCCTGGATC 
AAGGAAAAGA AGCTGCTGGT GAGCTCAGAG GACTACGGCC GTGACCTGAC GGGCGTCCAG 
AACCTGAGGA AGAAGCACAA ACGGCTGGAA GCAGAGCTGG CTGCCCACGA GCCGGCCATT 
CAGGGTGTCC TGGACACTGG AAAGAAGCTG TCTGATGACA ACACCATCGG GAAGGAGGAG 
ATTCAGCAGC GCCTGGCGCA GTTCGTGGAG CACTGGAAGG AGCTGAAGCA GCTGGCAGCT 
GCCCGGGGCC AGCGGCTAGA AGAATCTTTG GAATATCAGC AGTTTGTAGC CAACGTGGAG 
GAGGAGGAAG CCTGGATCAA TGAGAAGATG ACCCTGGTGG CCAGTGAAGA TTATGGAGAC 
ACTCTTGCTG CCATCCAGGG CTTGCTGAAG AAACACGAAG CCTTCGAGAC GGACTTCACT 
GTCCACAAGG ACCGAGTGAA TGATGTCTGC ACCAACGGCC AGGACCTGGT TAAGAAGAAC 
AATCACCATG AGGAGAACAT CTCTTCCAAG ATGAAGGGCT TGAACGGGAA AGTGTCAGAC 
TTGGAGAAGG CTGCCTCCCA GAGGAAGGCC AAGCTCGACG AGAACTCGGC CTTCCTCCAG 
TTCAACTGGA AAGCCGATGT GGTGGAGTCC TGGATCGGTG AAAAGGAGAA CAGCTTGAAG 
ACAGATGATT ATGGTCGAGA TCTGTCTTCT GTCCAAACTC TCCTCACCAA ACAGGAAACC 
TTCGATGCTG GCCTGCAGGC CTTCCAGCAG GAGGGCATCG CCAACATCAC TGCCCTCAAA 
GATCAGCTGC TGGCCGCCAA GCACGTTCAG TCCAAGGCCA TCGAGGCCCG CCATGCTGCC 
CTCATGAAGA GGTGGAGCCA GCTCCTGGCC AACTCGGCCA CCCGGAAGAA GAAGCTGCTG 
GAGGCACAGA GTCACTTCCG CAAGGTGGAG GATCTCTTCC TGACCTTTGC TAAAAAGGCT 
TCAGCCTTCA ACAGCTGGTT TGAAAACGCA GAGGAGGACT TAACCGACCC AGTGCGCTGC 
AACTCTCTGG AAGAGATCAA GGCCTTGAGA GAGGCCCACG ACGCCTTCCG CTCCTCGTTG 
AGCTCGGCCC AGGCCGACTT CAACCAGCTG GCAGAGCTGG ACCGCCAGAT CAAGAGCTTC 
CGCGTGGCCT CCAACCCCTA CACCTGGTTC ACCATGGAGG CCCTGGAGGA GACCTGGAGA 
AACCTACAGA AGATCATCAA GGAAAGGGAG CTGGAGCTGC AGAAGGAGCA GCGACGGCAG 
GAGGAGAATG ACAAGCTGCG CCAGGAGTTC GCCCAGCATG CCAACGCCTT CCACCAGTGG 
ATCCAGGAGA CCAGGTCCTG CATGGTGGAA GAATCCGGGA CCCTCGAATC ACAGCTTGAA 
GCTACCAAAC GCAAACACCA GGAGATTCGA GCTATGAGAA GTCAGCTCAA GAAGATTGAA 
GACCTGGGGG CGGCCATGGA GGAAGCGCTG ATCCTGGACA ACAAGTACAC GGAGCACAGC 
ACCGTGGGCC TGGCCCAGCA GTGGGACCAG CTCGACCAGT TGGGCATGCG CATGCAGCAC 
AACCTGGAGC AGCAGATCCA GGCCAGGAAC ACGACCGGAG TGACCGAGGA GGCCCTCAAA 
GAATTCAGCA TGATGTTCAA ACACTTCGAC AAGGACAAGT CTGGCCGGCT GAACCACCAG 
GAGTTCAAAT CCTGTCTGCG CTCCCTGGGC TATGACCTGC CCATGGTGGA GGAAGGGGAG 
CCCGACCCTG AGTTCGAGGC CATCCTGGAC ACTGTGGATC CTAACAGGGA CGGCCACGTC 
TCCCTGCAAG AGTACATGGC CTTTATGATC AGCCGTGAGA CCGAGAACGT CAAGTCCAGC 
GAGGAGATTG AGAGCGCCTT CCGGGCCCTG AGCTCTGAAG GGAAGCCTTA TGTGACCAAG 
GAGGAGCTGT ACCAGAACCT GACTCGGGAA CAAGCCGACT ACTGCGTCTC CCACATGAAG 
CCCTACGTGG ACGGCAAGGG CCGCGAACTC CCCACTGCTT TTGACTACGT CGAGTTCACC 
CGCTCGCTCT TCGTGAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854811.1
CDS	120..7460
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLHQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMYRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVDHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWESLKSKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGAMPRDESDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKEKLDVLDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQSFADQLIAGGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQSKHQKHQAFEAELHANADRIRGVIDMGNSLIDRGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDAINGRFQKIKSMAASRRARLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLVKKNNHHEENISSKMKGLNGKVSDLEKAASQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHAALMKRWSQLLANSATRKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_024999043.1
Organism	Bos taurus(cattle)
Definition	Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999043.1

ORF Insert Sequence:

ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT 
GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT 
CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC 
AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG 
AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC 
CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG 
AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT 
GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC 
CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC 
CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC 
CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT 
AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT 
GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG 
GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC 
ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC 
TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG 
AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG 
GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA 
GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG 
AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG 
CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG 
GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA 
ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG 
AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT 
GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT 
TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG 
CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT 
GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG 
AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG 
CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT 
CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC 
GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA 
GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG 
GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT 
CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG 
AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC 
AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC 
GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC 
CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC 
GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA 
GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC 
GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC 
AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC 
GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC 
AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGTACC ATTCTCTGCT GGAACTGGGA 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG 
AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC 
GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC 
AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA 
GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC 
AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG 
CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA 
GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC 
TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG 
GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC 
CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA 
TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC 
CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT 
CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG 
TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC 
GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG 
ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG 
GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG 
ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC 
GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG 
ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA 
GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG 
GACCCCACCA ACATCCAGAG CAAGCATCAG AAGCACCAGG CCTTCGAGGC AGAACTGCAC 
GCCAATGCGG ACCGGATCCG TGGGGTCATC GACATGGGCA ACTCCCTCAT TGACCGCGGG 
GCCTGCGCCG GCAGCGAGGA TGCCGTCAAG GCCCGGCTGG CCGCCTTAGC TGACCAGTGG 
CAGTTCCTGG TGCAGAAGTC GGCTGAGAAG AGCCAGAAGC TGAAAGAAGC CAACAAGCAG 
CAGAACTTCA ACACTGGCAT CAAGGACTTC GACTTCTGGC TCTCTGAGGT GGAAGCCCTG 
CTGGCCTCTG AGGATTATGG CAAAGACCTG GCTTCTGTGA ACAACTTGCT AAAAAAACAT 
CAGCTGCTGG AGGCAGATAT CTCTGCCCAC GAGGACCGCC TGAAGGACCT CAACAGCCAG 
GCGGACAGCC TGATGACCAG CAGCGCCTTC GACACCTCCC AGGTGAAGGA CAAGCGGGAC 
GCCATCAACG GCCGTTTCCA GAAGATCAAG AGCATGGCCG CCTCGAGGCG AGCTAGGCTG 
AATGAGTCCC ACCGCCTGCA CCAGTTTTTC CGGGACATGG ATGATGAGGA GTCCTGGATC 
AAGGAAAAGA AGCTGCTGGT GAGCTCAGAG GACTACGGCC GTGACCTGAC GGGCGTCCAG 
AACCTGAGGA AGAAGCACAA ACGGCTGGAA GCAGAGCTGG CTGCCCACGA GCCGGCCATT 
CAGGGTGTCC TGGACACTGG AAAGAAGCTG TCTGATGACA ACACCATCGG GAAGGAGGAG 
ATTCAGCAGC GCCTGGCGCA GTTCGTGGAG CACTGGAAGG AGCTGAAGCA GCTGGCAGCT 
GCCCGGGGCC AGCGGCTAGA AGAATCTTTG GAATATCAGC AGTTTGTAGC CAACGTGGAG 
GAGGAGGAAG CCTGGATCAA TGAGAAGATG ACCCTGGTGG CCAGTGAAGA TTATGGAGAC 
ACTCTTGCTG CCATCCAGGG CTTGCTGAAG AAACACGAAG CCTTCGAGAC GGACTTCACT 
GTCCACAAGG ACCGAGTGAA TGATGTCTGC ACCAACGGCC AGGACCTGGT TAAGAAGAAC 
AATCACCATG AGGAGAACAT CTCTTCCAAG ATGAAGGGCT TGAACGGGAA AGTGTCAGAC 
TTGGAGAAGG CTGCCTCCCA GAGGAAGGCC AAGCTCGACG AGAACTCGGC CTTCCTCCAG 
TTCAACTGGA AAGCCGATGT GGTGGAGTCC TGGATCGGTG AAAAGGAGAA CAGCTTGAAG 
ACAGATGATT ATGGTCGAGA TCTGTCTTCT GTCCAAACTC TCCTCACCAA ACAGGAAACC 
TTCGATGCTG GCCTGCAGGC CTTCCAGCAG GAGGGCATCG CCAACATCAC TGCCCTCAAA 
GATCAGCTGC TGGCCGCCAA GCACGTTCAG TCCAAGGCCA TCGAGGCCCG CCATGCTGCC 
CTCATGAAGA GGTGGAGCCA GCTCCTGGCC AACTCGGCCA CCCGGAAGAA GAAGCTGCTG 
GAGGCACAGA GTCACTTCCG CAAGGTGGAG GATCTCTTCC TGACCTTTGC TAAAAAGGCT 
TCAGCCTTCA ACAGCTGGTT TGAAAACGCA GAGGAGGACT TAACCGACCC AGTGCGCTGC 
AACTCTCTGG AAGAGATCAA GGCCTTGAGA GAGGCCCACG ACGCCTTCCG CTCCTCGTTG 
AGCTCGGCCC AGGCCGACTT CAACCAGCTG GCAGAGCTGG ACCGCCAGAT CAAGAGCTTC 
CGCGTGGCCT CCAACCCCTA CACCTGGTTC ACCATGGAGG CCCTGGAGGA GACCTGGAGA 
AACCTACAGA AGATCATCAA GGAAAGGGAG CTGGAGCTGC AGAAGGAGCA GCGACGGCAG 
GAGGAGAATG ACAAGCTGCG CCAGGAGTTC GCCCAGCATG CCAACGCCTT CCACCAGTGG 
ATCCAGGAGA CCAGGTCCTG CATGGTGGAA GAATCCGGGA CCCTCGAATC ACAGCTTGAA 
GCTACCAAAC GCAAACACCA GGAGATTCGA GCTATGAGAA GTCAGCTCAA GAAGATTGAA 
GACCTGGGGG CGGCCATGGA GGAAGCGCTG ATCCTGGACA ACAAGTACAC GGAGCACAGC 
ACCGTGGGCC TGGCCCAGCA GTGGGACCAG CTCGACCAGT TGGGCATGCG CATGCAGCAC 
AACCTGGAGC AGCAGATCCA GGCCAGGAAC ACGACCGGAG TGACCGAGGA GGCCCTCAAA 
GAATTCAGCA TGATGTTCAA ACACTTCGAC AAGGACAAGT CTGGCCGGCT GAACCACCAG 
GAGTTCAAAT CCTGTCTGCG CTCCCTGGGC TATGACCTGC CCATGGTGGA GGAAGGGGAG 
CCCGACCCTG AGTTCGAGGC CATCCTGGAC ACTGTGGATC CTAACAGGGA CGGCCACGTC 
TCCCTGCAAG AGTACATGGC CTTTATGATC AGCCGTGAGA CCGAGAACGT CAAGTCCAGC 
GAGGAGATTG AGAGCGCCTT CCGGGCCCTG AGCTCTGAAG GGAAGCCTTA TGTGACCAAG 
GAGGAGCTGT ACCAGAACCT GACTCGGGAA CAAGCCGACT ACTGCGTCTC CCACATGAAG 
CCCTACGTGG ACGGCAAGGG CCGCGAACTC CCCACTGCTT TTGACTACGT CGAGTTCACC 
CGCTCGCTCT TCGTGAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_024999044.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854812.1 Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X18, mRNA.

Price & Availability↑

CloneID	OBa220575
Clone ID Related Accession (Same CDS sequence)	XM_024999044.1
Accession Version	XM_024999044.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7326bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT 
GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT 
CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC 
AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG 
AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC 
CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG 
AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT 
GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC 
CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC 
CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC 
CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT 
AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT 
GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG 
GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC 
ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC 
TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG 
AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG 
GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA 
GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG 
AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG 
CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG 
GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA 
ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG 
AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT 
GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT 
TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG 
CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT 
GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG 
AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG 
CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT 
CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC 
GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA 
GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG 
GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT 
CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG 
AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC 
AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC 
GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC 
CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC 
GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA 
GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC 
GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC 
AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC 
GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC 
AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGTACC ATTCTCTGCT GGAACTGGGA 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG 
AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC 
GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC 
AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA 
GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC 
AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGG AGCTGAACGA GCGCTGGCGG 
TCCCTGCAGC AGCTGGCTGA GGAGCGGAGC CAACTCTTGG GCAGTGCCCA TGAAGTGCAG 
CGGTTCCACA GAGATGCTGA CGAAACCAAA GAGTGGATCG AAGAGAAGAA CCAGGCTCTA 
AACACAGACA ACTACGGCCA CGACCTGGCC AGTGTCCAGG CCCTGCAGCG CAAGCACGAG 
GGCTTCGAGA GGGACCTCGC AGCCCTTGGT GACAAGGTGA ACTCGCTCGG GGAGACAGCC 
GAGCGCCTGA TCCAGTCCCA CCCCGAGTCG GCAGAGGACC TGCAGGAGAA GTGCACTGAG 
CTGAACCAGG CATGGAGCAG CCTGGGCAAG CGTGCCGACC AGCGCAAGGC CAAGCTGGGC 
GACTCCCACG ACCTGCAGCG CTTCCTCAGC GACTTCCGGG ACCTCATGTC TTGGATCAAT 
GGAATCCGAG GTCTGGTGTC CTCAGACGAG CTAGCCAAGG ATGTCACCGG AGCTGAGGCC 
CTGCTGGAGC GACACCAGGA ACACCGGACA GAAATTGATG CCAGAGCTGG CACTTTCCAG 
GCATTCGAGC AGTTTGGGCA GCAGCTGTTG GCTCATGGGC ACTACGCCAG CCCCGAGATC 
AAGGAGAAGC TCGATGTTCT AGACCAGGAG CGTGCAGACC TTGAGAAGGC CTGGGTCCAG 
CGCAGGATGA TGCTGGATCA GTGCCTTGAA TTGCAGCTGT TCCATCGGGA TTGCGAGCAG 
GCCGAGAACT GGATGGCTGC CCGAGAGGCC TTTCTGAACA CAGAAGACAA AGGGGACTCG 
CTAGACAGTG TGGAGGCTCT GATCAAAAAA CACGAGGACT TTGATAAAGC AATTAATGTC 
CAGGAGGAGA AGATTGCTGC CCTGCAGTCC TTTGCTGACC AGCTCATCGC TGGTGGCCAC 
TACGCCAAAG GCGACATCTC CAGCCGGCGC AATGAGGTTC TGGACAGGTG GCGCCGTCTA 
AAAGCTCAGA TGATTGAGAA GAGGTCAAAG CTGGGAGAAT CACAGACCCT CCAGCAGTTC 
AGCCGGGATG TAGACGAGAT TGAAGCTTGG ATCAGTGAAA AATTACAGAC TGCAAGTGAT 
GAGTCATATA AGGACCCCAC CAACATCCAG CTTTCCAAGC TGCTGAGCAA GCATCAGAAG 
CACCAGGCCT TCGAGGCAGA ACTGCACGCC AATGCGGACC GGATCCGTGG GGTCATCGAC 
ATGGGCAACT CCCTCATTGA CCGCGGGGCC TGCGCCGGCA GCGAGGATGC CGTCAAGGCC 
CGGCTGGCCG CCTTAGCTGA CCAGTGGCAG TTCCTGGTGC AGAAGTCGGC TGAGAAGAGC 
CAGAAGCTGA AAGAAGCCAA CAAGCAGCAG AACTTCAACA CTGGCATCAA GGACTTCGAC 
TTCTGGCTCT CTGAGGTGGA AGCCCTGCTG GCCTCTGAGG ATTATGGCAA AGACCTGGCT 
TCTGTGAACA ACTTGCTAAA AAAACATCAG CTGCTGGAGG CAGATATCTC TGCCCACGAG 
GACCGCCTGA AGGACCTCAA CAGCCAGGCG GACAGCCTGA TGACCAGCAG CGCCTTCGAC 
ACCTCCCAGG TGAAGGACAA GCGGGACGCC ATCAACGGCC GTTTCCAGAA GATCAAGAGC 
ATGGCCGCCT CGAGGCGAGC TAGGCTGAAT GAGTCCCACC GCCTGCACCA GTTTTTCCGG 
GACATGGATG ATGAGGAGTC CTGGATCAAG GAAAAGAAGC TGCTGGTGAG CTCAGAGGAC 
TACGGCCGTG ACCTGACGGG CGTCCAGAAC CTGAGGAAGA AGCACAAACG GCTGGAAGCA 
GAGCTGGCTG CCCACGAGCC GGCCATTCAG GGTGTCCTGG ACACTGGAAA GAAGCTGTCT 
GATGACAACA CCATCGGGAA GGAGGAGATT CAGCAGCGCC TGGCGCAGTT CGTGGAGCAC 
TGGAAGGAGC TGAAGCAGCT GGCAGCTGCC CGGGGCCAGC GGCTAGAAGA ATCTTTGGAA 
TATCAGCAGT TTGTAGCCAA CGTGGAGGAG GAGGAAGCCT GGATCAATGA GAAGATGACC 
CTGGTGGCCA GTGAAGATTA TGGAGACACT CTTGCTGCCA TCCAGGGCTT GCTGAAGAAA 
CACGAAGCCT TCGAGACGGA CTTCACTGTC CACAAGGACC GAGTGAATGA TGTCTGCACC 
AACGGCCAGG ACCTGGTTAA GAAGAACAAT CACCATGAGG AGAACATCTC TTCCAAGATG 
AAGGGCTTGA ACGGGAAAGT GTCAGACTTG GAGAAGGCTG CCTCCCAGAG GAAGGCCAAG 
CTCGACGAGA ACTCGGCCTT CCTCCAGTTC AACTGGAAAG CCGATGTGGT GGAGTCCTGG 
ATCGGTGAAA AGGAGAACAG CTTGAAGACA GATGATTATG GTCGAGATCT GTCTTCTGTC 
CAAACTCTCC TCACCAAACA GGAAACCTTC GATGCTGGCC TGCAGGCCTT CCAGCAGGAG 
GGCATCGCCA ACATCACTGC CCTCAAAGAT CAGCTGCTGG CCGCCAAGCA CGTTCAGTCC 
AAGGCCATCG AGGCCCGCCA TGCTGCCCTC ATGAAGAGGT GGAGCCAGCT CCTGGCCAAC 
TCGGCCACCC GGAAGAAGAA GCTGCTGGAG GCACAGAGTC ACTTCCGCAA GGTGGAGGAT 
CTCTTCCTGA CCTTTGCTAA AAAGGCTTCA GCCTTCAACA GCTGGTTTGA AAACGCAGAG 
GAGGACTTAA CCGACCCAGT GCGCTGCAAC TCTCTGGAAG AGATCAAGGC CTTGAGAGAG 
GCCCACGACG CCTTCCGCTC CTCGTTGAGC TCGGCCCAGG CCGACTTCAA CCAGCTGGCA 
GAGCTGGACC GCCAGATCAA GAGCTTCCGC GTGGCCTCCA ACCCCTACAC CTGGTTCACC 
ATGGAGGCCC TGGAGGAGAC CTGGAGAAAC CTACAGAAGA TCATCAAGGA AAGGGAGCTG 
GAGCTGCAGA AGGAGCAGCG ACGGCAGGAG GAGAATGACA AGCTGCGCCA GGAGTTCGCC 
CAGCATGCCA ACGCCTTCCA CCAGTGGATC CAGGAGACCA GAACGTACCT CCTCGATGGG 
TCCTGCATGG TGGAAGAATC CGGGACCCTC GAATCACAGC TTGAAGCTAC CAAACGCAAA 
CACCAGGAGA TTCGAGCTAT GAGAAGTCAG CTCAAGAAGA TTGAAGACCT GGGGGCGGCC 
ATGGAGGAAG CGCTGATCCT GGACAACAAG TACACGGAGC ACAGCACCGT GGGCCTGGCC 
CAGCAGTGGG ACCAGCTCGA CCAGTTGGGC ATGCGCATGC AGCACAACCT GGAGCAGCAG 
ATCCAGGCCA GGAACACGAC CGGAGTGACC GAGGAGGCCC TCAAAGAATT CAGCATGATG 
TTCAAACACT TCGACAAGGA CAAGTCTGGC CGGCTGAACC ACCAGGAGTT CAAATCCTGT 
CTGCGCTCCC TGGGCTATGA CCTGCCCATG GTGGAGGAAG GGGAGCCCGA CCCTGAGTTC 
GAGGCCATCC TGGACACTGT GGATCCTAAC AGGGACGGCC ACGTCTCCCT GCAAGAGTAC 
ATGGCCTTTA TGATCAGCCG TGAGACCGAG AACGTCAAGT CCAGCGAGGA GATTGAGAGC 
GCCTTCCGGG CCCTGAGCTC TGAAGGGAAG CCTTATGTGA CCAAGGAGGA GCTGTACCAG 
AACCTGACTC GGGAACAAGC CGACTACTGC GTCTCCCACA TGAAGCCCTA CGTGGACGGC 
AAGGGCCGCG AACTCCCCAC TGCTTTTGAC TACGTCGAGT TCACCCGCTC GCTCTTCGTG 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854812.1
CDS	120..7445
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLHQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMYRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVDHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWESLKSKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGAMPRDESDSKTASPWKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKEKLDVLDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQSFADQLIAGGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDMGNSLIDRGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDAINGRFQKIKSMAASRRARLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLVKKNNHHEENISSKMKGLNGKVSDLEKAASQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHAALMKRWSQLLANSATRKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_024999044.1
Organism	Bos taurus(cattle)
Definition	Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999044.1

ORF Insert Sequence:

ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT 
GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT 
CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC 
AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG 
AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC 
CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG 
AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT 
GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC 
CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC 
CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC 
CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT 
AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT 
GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG 
GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC 
ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC 
TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG 
AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG 
GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA 
GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG 
AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG 
CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG 
GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA 
ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG 
AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT 
GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT 
TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG 
CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT 
GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG 
AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG 
CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT 
CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC 
GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA 
GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG 
GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT 
CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG 
AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC 
AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC 
GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC 
CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC 
GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA 
GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC 
GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC 
AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC 
GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC 
AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGTACC ATTCTCTGCT GGAACTGGGA 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG 
AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC 
GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC 
AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA 
GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC 
AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGG AGCTGAACGA GCGCTGGCGG 
TCCCTGCAGC AGCTGGCTGA GGAGCGGAGC CAACTCTTGG GCAGTGCCCA TGAAGTGCAG 
CGGTTCCACA GAGATGCTGA CGAAACCAAA GAGTGGATCG AAGAGAAGAA CCAGGCTCTA 
AACACAGACA ACTACGGCCA CGACCTGGCC AGTGTCCAGG CCCTGCAGCG CAAGCACGAG 
GGCTTCGAGA GGGACCTCGC AGCCCTTGGT GACAAGGTGA ACTCGCTCGG GGAGACAGCC 
GAGCGCCTGA TCCAGTCCCA CCCCGAGTCG GCAGAGGACC TGCAGGAGAA GTGCACTGAG 
CTGAACCAGG CATGGAGCAG CCTGGGCAAG CGTGCCGACC AGCGCAAGGC CAAGCTGGGC 
GACTCCCACG ACCTGCAGCG CTTCCTCAGC GACTTCCGGG ACCTCATGTC TTGGATCAAT 
GGAATCCGAG GTCTGGTGTC CTCAGACGAG CTAGCCAAGG ATGTCACCGG AGCTGAGGCC 
CTGCTGGAGC GACACCAGGA ACACCGGACA GAAATTGATG CCAGAGCTGG CACTTTCCAG 
GCATTCGAGC AGTTTGGGCA GCAGCTGTTG GCTCATGGGC ACTACGCCAG CCCCGAGATC 
AAGGAGAAGC TCGATGTTCT AGACCAGGAG CGTGCAGACC TTGAGAAGGC CTGGGTCCAG 
CGCAGGATGA TGCTGGATCA GTGCCTTGAA TTGCAGCTGT TCCATCGGGA TTGCGAGCAG 
GCCGAGAACT GGATGGCTGC CCGAGAGGCC TTTCTGAACA CAGAAGACAA AGGGGACTCG 
CTAGACAGTG TGGAGGCTCT GATCAAAAAA CACGAGGACT TTGATAAAGC AATTAATGTC 
CAGGAGGAGA AGATTGCTGC CCTGCAGTCC TTTGCTGACC AGCTCATCGC TGGTGGCCAC 
TACGCCAAAG GCGACATCTC CAGCCGGCGC AATGAGGTTC TGGACAGGTG GCGCCGTCTA 
AAAGCTCAGA TGATTGAGAA GAGGTCAAAG CTGGGAGAAT CACAGACCCT CCAGCAGTTC 
AGCCGGGATG TAGACGAGAT TGAAGCTTGG ATCAGTGAAA AATTACAGAC TGCAAGTGAT 
GAGTCATATA AGGACCCCAC CAACATCCAG CTTTCCAAGC TGCTGAGCAA GCATCAGAAG 
CACCAGGCCT TCGAGGCAGA ACTGCACGCC AATGCGGACC GGATCCGTGG GGTCATCGAC 
ATGGGCAACT CCCTCATTGA CCGCGGGGCC TGCGCCGGCA GCGAGGATGC CGTCAAGGCC 
CGGCTGGCCG CCTTAGCTGA CCAGTGGCAG TTCCTGGTGC AGAAGTCGGC TGAGAAGAGC 
CAGAAGCTGA AAGAAGCCAA CAAGCAGCAG AACTTCAACA CTGGCATCAA GGACTTCGAC 
TTCTGGCTCT CTGAGGTGGA AGCCCTGCTG GCCTCTGAGG ATTATGGCAA AGACCTGGCT 
TCTGTGAACA ACTTGCTAAA AAAACATCAG CTGCTGGAGG CAGATATCTC TGCCCACGAG 
GACCGCCTGA AGGACCTCAA CAGCCAGGCG GACAGCCTGA TGACCAGCAG CGCCTTCGAC 
ACCTCCCAGG TGAAGGACAA GCGGGACGCC ATCAACGGCC GTTTCCAGAA GATCAAGAGC 
ATGGCCGCCT CGAGGCGAGC TAGGCTGAAT GAGTCCCACC GCCTGCACCA GTTTTTCCGG 
GACATGGATG ATGAGGAGTC CTGGATCAAG GAAAAGAAGC TGCTGGTGAG CTCAGAGGAC 
TACGGCCGTG ACCTGACGGG CGTCCAGAAC CTGAGGAAGA AGCACAAACG GCTGGAAGCA 
GAGCTGGCTG CCCACGAGCC GGCCATTCAG GGTGTCCTGG ACACTGGAAA GAAGCTGTCT 
GATGACAACA CCATCGGGAA GGAGGAGATT CAGCAGCGCC TGGCGCAGTT CGTGGAGCAC 
TGGAAGGAGC TGAAGCAGCT GGCAGCTGCC CGGGGCCAGC GGCTAGAAGA ATCTTTGGAA 
TATCAGCAGT TTGTAGCCAA CGTGGAGGAG GAGGAAGCCT GGATCAATGA GAAGATGACC 
CTGGTGGCCA GTGAAGATTA TGGAGACACT CTTGCTGCCA TCCAGGGCTT GCTGAAGAAA 
CACGAAGCCT TCGAGACGGA CTTCACTGTC CACAAGGACC GAGTGAATGA TGTCTGCACC 
AACGGCCAGG ACCTGGTTAA GAAGAACAAT CACCATGAGG AGAACATCTC TTCCAAGATG 
AAGGGCTTGA ACGGGAAAGT GTCAGACTTG GAGAAGGCTG CCTCCCAGAG GAAGGCCAAG 
CTCGACGAGA ACTCGGCCTT CCTCCAGTTC AACTGGAAAG CCGATGTGGT GGAGTCCTGG 
ATCGGTGAAA AGGAGAACAG CTTGAAGACA GATGATTATG GTCGAGATCT GTCTTCTGTC 
CAAACTCTCC TCACCAAACA GGAAACCTTC GATGCTGGCC TGCAGGCCTT CCAGCAGGAG 
GGCATCGCCA ACATCACTGC CCTCAAAGAT CAGCTGCTGG CCGCCAAGCA CGTTCAGTCC 
AAGGCCATCG AGGCCCGCCA TGCTGCCCTC ATGAAGAGGT GGAGCCAGCT CCTGGCCAAC 
TCGGCCACCC GGAAGAAGAA GCTGCTGGAG GCACAGAGTC ACTTCCGCAA GGTGGAGGAT 
CTCTTCCTGA CCTTTGCTAA AAAGGCTTCA GCCTTCAACA GCTGGTTTGA AAACGCAGAG 
GAGGACTTAA CCGACCCAGT GCGCTGCAAC TCTCTGGAAG AGATCAAGGC CTTGAGAGAG 
GCCCACGACG CCTTCCGCTC CTCGTTGAGC TCGGCCCAGG CCGACTTCAA CCAGCTGGCA 
GAGCTGGACC GCCAGATCAA GAGCTTCCGC GTGGCCTCCA ACCCCTACAC CTGGTTCACC 
ATGGAGGCCC TGGAGGAGAC CTGGAGAAAC CTACAGAAGA TCATCAAGGA AAGGGAGCTG 
GAGCTGCAGA AGGAGCAGCG ACGGCAGGAG GAGAATGACA AGCTGCGCCA GGAGTTCGCC 
CAGCATGCCA ACGCCTTCCA CCAGTGGATC CAGGAGACCA GAACGTACCT CCTCGATGGG 
TCCTGCATGG TGGAAGAATC CGGGACCCTC GAATCACAGC TTGAAGCTAC CAAACGCAAA 
CACCAGGAGA TTCGAGCTAT GAGAAGTCAG CTCAAGAAGA TTGAAGACCT GGGGGCGGCC 
ATGGAGGAAG CGCTGATCCT GGACAACAAG TACACGGAGC ACAGCACCGT GGGCCTGGCC 
CAGCAGTGGG ACCAGCTCGA CCAGTTGGGC ATGCGCATGC AGCACAACCT GGAGCAGCAG 
ATCCAGGCCA GGAACACGAC CGGAGTGACC GAGGAGGCCC TCAAAGAATT CAGCATGATG 
TTCAAACACT TCGACAAGGA CAAGTCTGGC CGGCTGAACC ACCAGGAGTT CAAATCCTGT 
CTGCGCTCCC TGGGCTATGA CCTGCCCATG GTGGAGGAAG GGGAGCCCGA CCCTGAGTTC 
GAGGCCATCC TGGACACTGT GGATCCTAAC AGGGACGGCC ACGTCTCCCT GCAAGAGTAC 
ATGGCCTTTA TGATCAGCCG TGAGACCGAG AACGTCAAGT CCAGCGAGGA GATTGAGAGC 
GCCTTCCGGG CCCTGAGCTC TGAAGGGAAG CCTTATGTGA CCAAGGAGGA GCTGTACCAG 
AACCTGACTC GGGAACAAGC CGACTACTGC GTCTCCCACA TGAAGCCCTA CGTGGACGGC 
AAGGGCCGCG AACTCCCCAC TGCTTTTGAC TACGTCGAGT TCACCCGCTC GCTCTTCGTG 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_024999045.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854813.1 Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X19, mRNA.

Price & Availability↑

CloneID	OBa220576
Clone ID Related Accession (Same CDS sequence)	XM_024999045.1
Accession Version	XM_024999045.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7311bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT 
GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT 
CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC 
AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG 
AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC 
CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG 
AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT 
GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC 
CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC 
CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC 
CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT 
AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT 
GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG 
GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC 
ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC 
TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG 
AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG 
GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA 
GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG 
AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG 
CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG 
GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA 
ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG 
AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT 
GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT 
TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG 
CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT 
GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG 
AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG 
CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT 
CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC 
GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA 
GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG 
GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT 
CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG 
AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC 
AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC 
GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC 
CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC 
GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA 
GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC 
GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC 
AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC 
GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC 
AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGTACC ATTCTCTGCT GGAACTGGGA 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG 
AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC 
GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC 
AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA 
GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC 
AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGG AGCTGAACGA GCGCTGGCGG 
TCCCTGCAGC AGCTGGCTGA GGAGCGGAGC CAACTCTTGG GCAGTGCCCA TGAAGTGCAG 
CGGTTCCACA GAGATGCTGA CGAAACCAAA GAGTGGATCG AAGAGAAGAA CCAGGCTCTA 
AACACAGACA ACTACGGCCA CGACCTGGCC AGTGTCCAGG CCCTGCAGCG CAAGCACGAG 
GGCTTCGAGA GGGACCTCGC AGCCCTTGGT GACAAGGTGA ACTCGCTCGG GGAGACAGCC 
GAGCGCCTGA TCCAGTCCCA CCCCGAGTCG GCAGAGGACC TGCAGGAGAA GTGCACTGAG 
CTGAACCAGG CATGGAGCAG CCTGGGCAAG CGTGCCGACC AGCGCAAGGC CAAGCTGGGC 
GACTCCCACG ACCTGCAGCG CTTCCTCAGC GACTTCCGGG ACCTCATGTC TTGGATCAAT 
GGAATCCGAG GTCTGGTGTC CTCAGACGAG CTAGCCAAGG ATGTCACCGG AGCTGAGGCC 
CTGCTGGAGC GACACCAGGA ACACCGGACA GAAATTGATG CCAGAGCTGG CACTTTCCAG 
GCATTCGAGC AGTTTGGGCA GCAGCTGTTG GCTCATGGGC ACTACGCCAG CCCCGAGATC 
AAGGAGAAGC TCGATGTTCT AGACCAGGAG CGTGCAGACC TTGAGAAGGC CTGGGTCCAG 
CGCAGGATGA TGCTGGATCA GTGCCTTGAA TTGCAGCTGT TCCATCGGGA TTGCGAGCAG 
GCCGAGAACT GGATGGCTGC CCGAGAGGCC TTTCTGAACA CAGAAGACAA AGGGGACTCG 
CTAGACAGTG TGGAGGCTCT GATCAAAAAA CACGAGGACT TTGATAAAGC AATTAATGTC 
CAGGAGGAGA AGATTGCTGC CCTGCAGTCC TTTGCTGACC AGCTCATCGC TGGTGGCCAC 
TACGCCAAAG GCGACATCTC CAGCCGGCGC AATGAGGTTC TGGACAGGTG GCGCCGTCTA 
AAAGCTCAGA TGATTGAGAA GAGGTCAAAG CTGGGAGAAT CACAGACCCT CCAGCAGTTC 
AGCCGGGATG TAGACGAGAT TGAAGCTTGG ATCAGTGAAA AATTACAGAC TGCAAGTGAT 
GAGTCATATA AGGACCCCAC CAACATCCAG AGCAAGCATC AGAAGCACCA GGCCTTCGAG 
GCAGAACTGC ACGCCAATGC GGACCGGATC CGTGGGGTCA TCGACATGGG CAACTCCCTC 
ATTGACCGCG GGGCCTGCGC CGGCAGCGAG GATGCCGTCA AGGCCCGGCT GGCCGCCTTA 
GCTGACCAGT GGCAGTTCCT GGTGCAGAAG TCGGCTGAGA AGAGCCAGAA GCTGAAAGAA 
GCCAACAAGC AGCAGAACTT CAACACTGGC ATCAAGGACT TCGACTTCTG GCTCTCTGAG 
GTGGAAGCCC TGCTGGCCTC TGAGGATTAT GGCAAAGACC TGGCTTCTGT GAACAACTTG 
CTAAAAAAAC ATCAGCTGCT GGAGGCAGAT ATCTCTGCCC ACGAGGACCG CCTGAAGGAC 
CTCAACAGCC AGGCGGACAG CCTGATGACC AGCAGCGCCT TCGACACCTC CCAGGTGAAG 
GACAAGCGGG ACGCCATCAA CGGCCGTTTC CAGAAGATCA AGAGCATGGC CGCCTCGAGG 
CGAGCTAGGC TGAATGAGTC CCACCGCCTG CACCAGTTTT TCCGGGACAT GGATGATGAG 
GAGTCCTGGA TCAAGGAAAA GAAGCTGCTG GTGAGCTCAG AGGACTACGG CCGTGACCTG 
ACGGGCGTCC AGAACCTGAG GAAGAAGCAC AAACGGCTGG AAGCAGAGCT GGCTGCCCAC 
GAGCCGGCCA TTCAGGGTGT CCTGGACACT GGAAAGAAGC TGTCTGATGA CAACACCATC 
GGGAAGGAGG AGATTCAGCA GCGCCTGGCG CAGTTCGTGG AGCACTGGAA GGAGCTGAAG 
CAGCTGGCAG CTGCCCGGGG CCAGCGGCTA GAAGAATCTT TGGAATATCA GCAGTTTGTA 
GCCAACGTGG AGGAGGAGGA AGCCTGGATC AATGAGAAGA TGACCCTGGT GGCCAGTGAA 
GATTATGGAG ACACTCTTGC TGCCATCCAG GGCTTGCTGA AGAAACACGA AGCCTTCGAG 
ACGGACTTCA CTGTCCACAA GGACCGAGTG AATGATGTCT GCACCAACGG CCAGGACCTG 
GTTAAGAAGA ACAATCACCA TGAGGAGAAC ATCTCTTCCA AGATGAAGGG CTTGAACGGG 
AAAGTGTCAG ACTTGGAGAA GGCTGCCTCC CAGAGGAAGG CCAAGCTCGA CGAGAACTCG 
GCCTTCCTCC AGTTCAACTG GAAAGCCGAT GTGGTGGAGT CCTGGATCGG TGAAAAGGAG 
AACAGCTTGA AGACAGATGA TTATGGTCGA GATCTGTCTT CTGTCCAAAC TCTCCTCACC 
AAACAGGAAA CCTTCGATGC TGGCCTGCAG GCCTTCCAGC AGGAGGGCAT CGCCAACATC 
ACTGCCCTCA AAGATCAGCT GCTGGCCGCC AAGCACGTTC AGTCCAAGGC CATCGAGGCC 
CGCCATGCTG CCCTCATGAA GAGGTGGAGC CAGCTCCTGG CCAACTCGGC CACCCGGAAG 
AAGAAGCTGC TGGAGGCACA GAGTCACTTC CGCAAGGTGG AGGATCTCTT CCTGACCTTT 
GCTAAAAAGG CTTCAGCCTT CAACAGCTGG TTTGAAAACG CAGAGGAGGA CTTAACCGAC 
CCAGTGCGCT GCAACTCTCT GGAAGAGATC AAGGCCTTGA GAGAGGCCCA CGACGCCTTC 
CGCTCCTCGT TGAGCTCGGC CCAGGCCGAC TTCAACCAGC TGGCAGAGCT GGACCGCCAG 
ATCAAGAGCT TCCGCGTGGC CTCCAACCCC TACACCTGGT TCACCATGGA GGCCCTGGAG 
GAGACCTGGA GAAACCTACA GAAGATCATC AAGGAAAGGG AGCTGGAGCT GCAGAAGGAG 
CAGCGACGGC AGGAGGAGAA TGACAAGCTG CGCCAGGAGT TCGCCCAGCA TGCCAACGCC 
TTCCACCAGT GGATCCAGGA GACCAGAACG TACCTCCTCG ATGGGTCCTG CATGGTGGAA 
GAATCCGGGA CCCTCGAATC ACAGCTTGAA GCTACCAAAC GCAAACACCA GGAGATTCGA 
GCTATGAGAA GTCAGCTCAA GAAGATTGAA GACCTGGGGG CGGCCATGGA GGAAGCGCTG 
ATCCTGGACA ACAAGTACAC GGAGCACAGC ACCGTGGGCC TGGCCCAGCA GTGGGACCAG 
CTCGACCAGT TGGGCATGCG CATGCAGCAC AACCTGGAGC AGCAGATCCA GGCCAGGAAC 
ACGACCGGAG TGACCGAGGA GGCCCTCAAA GAATTCAGCA TGATGTTCAA ACACTTCGAC 
AAGGACAAGT CTGGCCGGCT GAACCACCAG GAGTTCAAAT CCTGTCTGCG CTCCCTGGGC 
TATGACCTGC CCATGGTGGA GGAAGGGGAG CCCGACCCTG AGTTCGAGGC CATCCTGGAC 
ACTGTGGATC CTAACAGGGA CGGCCACGTC TCCCTGCAAG AGTACATGGC CTTTATGATC 
AGCCGTGAGA CCGAGAACGT CAAGTCCAGC GAGGAGATTG AGAGCGCCTT CCGGGCCCTG 
AGCTCTGAAG GGAAGCCTTA TGTGACCAAG GAGGAGCTGT ACCAGAACCT GACTCGGGAA 
CAAGCCGACT ACTGCGTCTC CCACATGAAG CCCTACGTGG ACGGCAAGGG CCGCGAACTC 
CCCACTGCTT TTGACTACGT CGAGTTCACC CGCTCGCTCT TCGTGAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854813.1
CDS	120..7430
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLHQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMYRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVDHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWESLKSKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGAMPRDESDSKTASPWKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKEKLDVLDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQSFADQLIAGGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQSKHQKHQAFEAELHANADRIRGVIDMGNSLIDRGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDAINGRFQKIKSMAASRRARLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLVKKNNHHEENISSKMKGLNGKVSDLEKAASQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHAALMKRWSQLLANSATRKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_024999045.1
Organism	Bos taurus(cattle)
Definition	Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999045.1

ORF Insert Sequence:

ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT 
GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT 
CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC 
AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG 
AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC 
CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG 
AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT 
GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC 
CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC 
CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC 
CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT 
AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT 
GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG 
GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC 
ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC 
TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG 
AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG 
GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA 
GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG 
AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG 
CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG 
GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA 
ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG 
AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT 
GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT 
TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG 
CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT 
GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG 
AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG 
CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT 
CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC 
GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA 
GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG 
GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT 
CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG 
AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC 
AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC 
GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC 
CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC 
GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA 
GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC 
GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC 
AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC 
GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC 
AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGTACC ATTCTCTGCT GGAACTGGGA 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG 
AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC 
GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC 
AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA 
GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC 
AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGG AGCTGAACGA GCGCTGGCGG 
TCCCTGCAGC AGCTGGCTGA GGAGCGGAGC CAACTCTTGG GCAGTGCCCA TGAAGTGCAG 
CGGTTCCACA GAGATGCTGA CGAAACCAAA GAGTGGATCG AAGAGAAGAA CCAGGCTCTA 
AACACAGACA ACTACGGCCA CGACCTGGCC AGTGTCCAGG CCCTGCAGCG CAAGCACGAG 
GGCTTCGAGA GGGACCTCGC AGCCCTTGGT GACAAGGTGA ACTCGCTCGG GGAGACAGCC 
GAGCGCCTGA TCCAGTCCCA CCCCGAGTCG GCAGAGGACC TGCAGGAGAA GTGCACTGAG 
CTGAACCAGG CATGGAGCAG CCTGGGCAAG CGTGCCGACC AGCGCAAGGC CAAGCTGGGC 
GACTCCCACG ACCTGCAGCG CTTCCTCAGC GACTTCCGGG ACCTCATGTC TTGGATCAAT 
GGAATCCGAG GTCTGGTGTC CTCAGACGAG CTAGCCAAGG ATGTCACCGG AGCTGAGGCC 
CTGCTGGAGC GACACCAGGA ACACCGGACA GAAATTGATG CCAGAGCTGG CACTTTCCAG 
GCATTCGAGC AGTTTGGGCA GCAGCTGTTG GCTCATGGGC ACTACGCCAG CCCCGAGATC 
AAGGAGAAGC TCGATGTTCT AGACCAGGAG CGTGCAGACC TTGAGAAGGC CTGGGTCCAG 
CGCAGGATGA TGCTGGATCA GTGCCTTGAA TTGCAGCTGT TCCATCGGGA TTGCGAGCAG 
GCCGAGAACT GGATGGCTGC CCGAGAGGCC TTTCTGAACA CAGAAGACAA AGGGGACTCG 
CTAGACAGTG TGGAGGCTCT GATCAAAAAA CACGAGGACT TTGATAAAGC AATTAATGTC 
CAGGAGGAGA AGATTGCTGC CCTGCAGTCC TTTGCTGACC AGCTCATCGC TGGTGGCCAC 
TACGCCAAAG GCGACATCTC CAGCCGGCGC AATGAGGTTC TGGACAGGTG GCGCCGTCTA 
AAAGCTCAGA TGATTGAGAA GAGGTCAAAG CTGGGAGAAT CACAGACCCT CCAGCAGTTC 
AGCCGGGATG TAGACGAGAT TGAAGCTTGG ATCAGTGAAA AATTACAGAC TGCAAGTGAT 
GAGTCATATA AGGACCCCAC CAACATCCAG AGCAAGCATC AGAAGCACCA GGCCTTCGAG 
GCAGAACTGC ACGCCAATGC GGACCGGATC CGTGGGGTCA TCGACATGGG CAACTCCCTC 
ATTGACCGCG GGGCCTGCGC CGGCAGCGAG GATGCCGTCA AGGCCCGGCT GGCCGCCTTA 
GCTGACCAGT GGCAGTTCCT GGTGCAGAAG TCGGCTGAGA AGAGCCAGAA GCTGAAAGAA 
GCCAACAAGC AGCAGAACTT CAACACTGGC ATCAAGGACT TCGACTTCTG GCTCTCTGAG 
GTGGAAGCCC TGCTGGCCTC TGAGGATTAT GGCAAAGACC TGGCTTCTGT GAACAACTTG 
CTAAAAAAAC ATCAGCTGCT GGAGGCAGAT ATCTCTGCCC ACGAGGACCG CCTGAAGGAC 
CTCAACAGCC AGGCGGACAG CCTGATGACC AGCAGCGCCT TCGACACCTC CCAGGTGAAG 
GACAAGCGGG ACGCCATCAA CGGCCGTTTC CAGAAGATCA AGAGCATGGC CGCCTCGAGG 
CGAGCTAGGC TGAATGAGTC CCACCGCCTG CACCAGTTTT TCCGGGACAT GGATGATGAG 
GAGTCCTGGA TCAAGGAAAA GAAGCTGCTG GTGAGCTCAG AGGACTACGG CCGTGACCTG 
ACGGGCGTCC AGAACCTGAG GAAGAAGCAC AAACGGCTGG AAGCAGAGCT GGCTGCCCAC 
GAGCCGGCCA TTCAGGGTGT CCTGGACACT GGAAAGAAGC TGTCTGATGA CAACACCATC 
GGGAAGGAGG AGATTCAGCA GCGCCTGGCG CAGTTCGTGG AGCACTGGAA GGAGCTGAAG 
CAGCTGGCAG CTGCCCGGGG CCAGCGGCTA GAAGAATCTT TGGAATATCA GCAGTTTGTA 
GCCAACGTGG AGGAGGAGGA AGCCTGGATC AATGAGAAGA TGACCCTGGT GGCCAGTGAA 
GATTATGGAG ACACTCTTGC TGCCATCCAG GGCTTGCTGA AGAAACACGA AGCCTTCGAG 
ACGGACTTCA CTGTCCACAA GGACCGAGTG AATGATGTCT GCACCAACGG CCAGGACCTG 
GTTAAGAAGA ACAATCACCA TGAGGAGAAC ATCTCTTCCA AGATGAAGGG CTTGAACGGG 
AAAGTGTCAG ACTTGGAGAA GGCTGCCTCC CAGAGGAAGG CCAAGCTCGA CGAGAACTCG 
GCCTTCCTCC AGTTCAACTG GAAAGCCGAT GTGGTGGAGT CCTGGATCGG TGAAAAGGAG 
AACAGCTTGA AGACAGATGA TTATGGTCGA GATCTGTCTT CTGTCCAAAC TCTCCTCACC 
AAACAGGAAA CCTTCGATGC TGGCCTGCAG GCCTTCCAGC AGGAGGGCAT CGCCAACATC 
ACTGCCCTCA AAGATCAGCT GCTGGCCGCC AAGCACGTTC AGTCCAAGGC CATCGAGGCC 
CGCCATGCTG CCCTCATGAA GAGGTGGAGC CAGCTCCTGG CCAACTCGGC CACCCGGAAG 
AAGAAGCTGC TGGAGGCACA GAGTCACTTC CGCAAGGTGG AGGATCTCTT CCTGACCTTT 
GCTAAAAAGG CTTCAGCCTT CAACAGCTGG TTTGAAAACG CAGAGGAGGA CTTAACCGAC 
CCAGTGCGCT GCAACTCTCT GGAAGAGATC AAGGCCTTGA GAGAGGCCCA CGACGCCTTC 
CGCTCCTCGT TGAGCTCGGC CCAGGCCGAC TTCAACCAGC TGGCAGAGCT GGACCGCCAG 
ATCAAGAGCT TCCGCGTGGC CTCCAACCCC TACACCTGGT TCACCATGGA GGCCCTGGAG 
GAGACCTGGA GAAACCTACA GAAGATCATC AAGGAAAGGG AGCTGGAGCT GCAGAAGGAG 
CAGCGACGGC AGGAGGAGAA TGACAAGCTG CGCCAGGAGT TCGCCCAGCA TGCCAACGCC 
TTCCACCAGT GGATCCAGGA GACCAGAACG TACCTCCTCG ATGGGTCCTG CATGGTGGAA 
GAATCCGGGA CCCTCGAATC ACAGCTTGAA GCTACCAAAC GCAAACACCA GGAGATTCGA 
GCTATGAGAA GTCAGCTCAA GAAGATTGAA GACCTGGGGG CGGCCATGGA GGAAGCGCTG 
ATCCTGGACA ACAAGTACAC GGAGCACAGC ACCGTGGGCC TGGCCCAGCA GTGGGACCAG 
CTCGACCAGT TGGGCATGCG CATGCAGCAC AACCTGGAGC AGCAGATCCA GGCCAGGAAC 
ACGACCGGAG TGACCGAGGA GGCCCTCAAA GAATTCAGCA TGATGTTCAA ACACTTCGAC 
AAGGACAAGT CTGGCCGGCT GAACCACCAG GAGTTCAAAT CCTGTCTGCG CTCCCTGGGC 
TATGACCTGC CCATGGTGGA GGAAGGGGAG CCCGACCCTG AGTTCGAGGC CATCCTGGAC 
ACTGTGGATC CTAACAGGGA CGGCCACGTC TCCCTGCAAG AGTACATGGC CTTTATGATC 
AGCCGTGAGA CCGAGAACGT CAAGTCCAGC GAGGAGATTG AGAGCGCCTT CCGGGCCCTG 
AGCTCTGAAG GGAAGCCTTA TGTGACCAAG GAGGAGCTGT ACCAGAACCT GACTCGGGAA 
CAAGCCGACT ACTGCGTCTC CCACATGAAG CCCTACGTGG ACGGCAAGGG CCGCGAACTC 
CCCACTGCTT TTGACTACGT CGAGTTCACC CGCTCGCTCT TCGTGAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_024999046.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854814.1 Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X20, mRNA.

Price & Availability↑

CloneID	OBa220577
Clone ID Related Accession (Same CDS sequence)	XM_024999046.1
Accession Version	XM_024999046.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7293bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT 
GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT 
CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC 
AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG 
AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC 
CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG 
AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT 
GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC 
CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC 
CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC 
CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT 
AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT 
GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG 
GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC 
ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC 
TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG 
AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG 
GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA 
GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG 
AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG 
CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG 
GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA 
ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG 
AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT 
GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT 
TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG 
CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT 
GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG 
AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG 
CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT 
CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC 
GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA 
GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG 
GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT 
CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG 
AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC 
AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC 
GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC 
CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC 
GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA 
GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC 
GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC 
AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC 
GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC 
AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGTACC ATTCTCTGCT GGAACTGGGA 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG 
AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC 
GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC 
AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA 
GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC 
AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGG AGCTGAACGA GCGCTGGCGG 
TCCCTGCAGC AGCTGGCTGA GGAGCGGAGC CAACTCTTGG GCAGTGCCCA TGAAGTGCAG 
CGGTTCCACA GAGATGCTGA CGAAACCAAA GAGTGGATCG AAGAGAAGAA CCAGGCTCTA 
AACACAGACA ACTACGGCCA CGACCTGGCC AGTGTCCAGG CCCTGCAGCG CAAGCACGAG 
GGCTTCGAGA GGGACCTCGC AGCCCTTGGT GACAAGGTGA ACTCGCTCGG GGAGACAGCC 
GAGCGCCTGA TCCAGTCCCA CCCCGAGTCG GCAGAGGACC TGCAGGAGAA GTGCACTGAG 
CTGAACCAGG CATGGAGCAG CCTGGGCAAG CGTGCCGACC AGCGCAAGGC CAAGCTGGGC 
GACTCCCACG ACCTGCAGCG CTTCCTCAGC GACTTCCGGG ACCTCATGTC TTGGATCAAT 
GGAATCCGAG GTCTGGTGTC CTCAGACGAG CTAGCCAAGG ATGTCACCGG AGCTGAGGCC 
CTGCTGGAGC GACACCAGGA ACACCGGACA GAAATTGATG CCAGAGCTGG CACTTTCCAG 
GCATTCGAGC AGTTTGGGCA GCAGCTGTTG GCTCATGGGC ACTACGCCAG CCCCGAGATC 
AAGGAGAAGC TCGATGTTCT AGACCAGGAG CGTGCAGACC TTGAGAAGGC CTGGGTCCAG 
CGCAGGATGA TGCTGGATCA GTGCCTTGAA TTGCAGCTGT TCCATCGGGA TTGCGAGCAG 
GCCGAGAACT GGATGGCTGC CCGAGAGGCC TTTCTGAACA CAGAAGACAA AGGGGACTCG 
CTAGACAGTG TGGAGGCTCT GATCAAAAAA CACGAGGACT TTGATAAAGC AATTAATGTC 
CAGGAGGAGA AGATTGCTGC CCTGCAGTCC TTTGCTGACC AGCTCATCGC TGGTGGCCAC 
TACGCCAAAG GCGACATCTC CAGCCGGCGC AATGAGGTTC TGGACAGGTG GCGCCGTCTA 
AAAGCTCAGA TGATTGAGAA GAGGTCAAAG CTGGGAGAAT CACAGACCCT CCAGCAGTTC 
AGCCGGGATG TAGACGAGAT TGAAGCTTGG ATCAGTGAAA AATTACAGAC TGCAAGTGAT 
GAGTCATATA AGGACCCCAC CAACATCCAG AGCAAGCATC AGAAGCACCA GGCCTTCGAG 
GCAGAACTGC ACGCCAATGC GGACCGGATC CGTGGGGTCA TCGACATGGG CAACTCCCTC 
ATTGACCGCG GGGCCTGCGC CGGCAGCGAG GATGCCGTCA AGGCCCGGCT GGCCGCCTTA 
GCTGACCAGT GGCAGTTCCT GGTGCAGAAG TCGGCTGAGA AGAGCCAGAA GCTGAAAGAA 
GCCAACAAGC AGCAGAACTT CAACACTGGC ATCAAGGACT TCGACTTCTG GCTCTCTGAG 
GTGGAAGCCC TGCTGGCCTC TGAGGATTAT GGCAAAGACC TGGCTTCTGT GAACAACTTG 
CTAAAAAAAC ATCAGCTGCT GGAGGCAGAT ATCTCTGCCC ACGAGGACCG CCTGAAGGAC 
CTCAACAGCC AGGCGGACAG CCTGATGACC AGCAGCGCCT TCGACACCTC CCAGGTGAAG 
GACAAGCGGG ACGCCATCAA CGGCCGTTTC CAGAAGATCA AGAGCATGGC CGCCTCGAGG 
CGAGCTAGGC TGAATGAGTC CCACCGCCTG CACCAGTTTT TCCGGGACAT GGATGATGAG 
GAGTCCTGGA TCAAGGAAAA GAAGCTGCTG GTGAGCTCAG AGGACTACGG CCGTGACCTG 
ACGGGCGTCC AGAACCTGAG GAAGAAGCAC AAACGGCTGG AAGCAGAGCT GGCTGCCCAC 
GAGCCGGCCA TTCAGGGTGT CCTGGACACT GGAAAGAAGC TGTCTGATGA CAACACCATC 
GGGAAGGAGG AGATTCAGCA GCGCCTGGCG CAGTTCGTGG AGCACTGGAA GGAGCTGAAG 
CAGCTGGCAG CTGCCCGGGG CCAGCGGCTA GAAGAATCTT TGGAATATCA GCAGTTTGTA 
GCCAACGTGG AGGAGGAGGA AGCCTGGATC AATGAGAAGA TGACCCTGGT GGCCAGTGAA 
GATTATGGAG ACACTCTTGC TGCCATCCAG GGCTTGCTGA AGAAACACGA AGCCTTCGAG 
ACGGACTTCA CTGTCCACAA GGACCGAGTG AATGATGTCT GCACCAACGG CCAGGACCTG 
GTTAAGAAGA ACAATCACCA TGAGGAGAAC ATCTCTTCCA AGATGAAGGG CTTGAACGGG 
AAAGTGTCAG ACTTGGAGAA GGCTGCCTCC CAGAGGAAGG CCAAGCTCGA CGAGAACTCG 
GCCTTCCTCC AGTTCAACTG GAAAGCCGAT GTGGTGGAGT CCTGGATCGG TGAAAAGGAG 
AACAGCTTGA AGACAGATGA TTATGGTCGA GATCTGTCTT CTGTCCAAAC TCTCCTCACC 
AAACAGGAAA CCTTCGATGC TGGCCTGCAG GCCTTCCAGC AGGAGGGCAT CGCCAACATC 
ACTGCCCTCA AAGATCAGCT GCTGGCCGCC AAGCACGTTC AGTCCAAGGC CATCGAGGCC 
CGCCATGCTG CCCTCATGAA GAGGTGGAGC CAGCTCCTGG CCAACTCGGC CACCCGGAAG 
AAGAAGCTGC TGGAGGCACA GAGTCACTTC CGCAAGGTGG AGGATCTCTT CCTGACCTTT 
GCTAAAAAGG CTTCAGCCTT CAACAGCTGG TTTGAAAACG CAGAGGAGGA CTTAACCGAC 
CCAGTGCGCT GCAACTCTCT GGAAGAGATC AAGGCCTTGA GAGAGGCCCA CGACGCCTTC 
CGCTCCTCGT TGAGCTCGGC CCAGGCCGAC TTCAACCAGC TGGCAGAGCT GGACCGCCAG 
ATCAAGAGCT TCCGCGTGGC CTCCAACCCC TACACCTGGT TCACCATGGA GGCCCTGGAG 
GAGACCTGGA GAAACCTACA GAAGATCATC AAGGAAAGGG AGCTGGAGCT GCAGAAGGAG 
CAGCGACGGC AGGAGGAGAA TGACAAGCTG CGCCAGGAGT TCGCCCAGCA TGCCAACGCC 
TTCCACCAGT GGATCCAGGA GACCAGGTCC TGCATGGTGG AAGAATCCGG GACCCTCGAA 
TCACAGCTTG AAGCTACCAA ACGCAAACAC CAGGAGATTC GAGCTATGAG AAGTCAGCTC 
AAGAAGATTG AAGACCTGGG GGCGGCCATG GAGGAAGCGC TGATCCTGGA CAACAAGTAC 
ACGGAGCACA GCACCGTGGG CCTGGCCCAG CAGTGGGACC AGCTCGACCA GTTGGGCATG 
CGCATGCAGC ACAACCTGGA GCAGCAGATC CAGGCCAGGA ACACGACCGG AGTGACCGAG 
GAGGCCCTCA AAGAATTCAG CATGATGTTC AAACACTTCG ACAAGGACAA GTCTGGCCGG 
CTGAACCACC AGGAGTTCAA ATCCTGTCTG CGCTCCCTGG GCTATGACCT GCCCATGGTG 
GAGGAAGGGG AGCCCGACCC TGAGTTCGAG GCCATCCTGG ACACTGTGGA TCCTAACAGG 
GACGGCCACG TCTCCCTGCA AGAGTACATG GCCTTTATGA TCAGCCGTGA GACCGAGAAC 
GTCAAGTCCA GCGAGGAGAT TGAGAGCGCC TTCCGGGCCC TGAGCTCTGA AGGGAAGCCT 
TATGTGACCA AGGAGGAGCT GTACCAGAAC CTGACTCGGG AACAAGCCGA CTACTGCGTC 
TCCCACATGA AGCCCTACGT GGACGGCAAG GGCCGCGAAC TCCCCACTGC TTTTGACTAC 
GTCGAGTTCA CCCGCTCGCT CTTCGTGAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854814.1
CDS	120..7412
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLHQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMYRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVDHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWESLKSKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGAMPRDESDSKTASPWKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKEKLDVLDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQSFADQLIAGGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQSKHQKHQAFEAELHANADRIRGVIDMGNSLIDRGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDAINGRFQKIKSMAASRRARLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLVKKNNHHEENISSKMKGLNGKVSDLEKAASQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHAALMKRWSQLLANSATRKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_024999046.1
Organism	Bos taurus(cattle)
Definition	Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999046.1

ORF Insert Sequence:

ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT 
GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT 
CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC 
AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG 
AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC 
CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG 
AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT 
GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC 
CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC 
CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC 
CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT 
AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT 
GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG 
GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC 
ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC 
TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG 
AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG 
GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA 
GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG 
AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG 
CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG 
GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA 
ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG 
AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT 
GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT 
TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG 
CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT 
GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG 
AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG 
CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT 
CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC 
GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA 
GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG 
GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT 
CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG 
AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC 
AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC 
GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC 
CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC 
GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA 
GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC 
GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC 
AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC 
GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC 
AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGTACC ATTCTCTGCT GGAACTGGGA 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG 
AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC 
GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC 
AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA 
GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC 
AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGG AGCTGAACGA GCGCTGGCGG 
TCCCTGCAGC AGCTGGCTGA GGAGCGGAGC CAACTCTTGG GCAGTGCCCA TGAAGTGCAG 
CGGTTCCACA GAGATGCTGA CGAAACCAAA GAGTGGATCG AAGAGAAGAA CCAGGCTCTA 
AACACAGACA ACTACGGCCA CGACCTGGCC AGTGTCCAGG CCCTGCAGCG CAAGCACGAG 
GGCTTCGAGA GGGACCTCGC AGCCCTTGGT GACAAGGTGA ACTCGCTCGG GGAGACAGCC 
GAGCGCCTGA TCCAGTCCCA CCCCGAGTCG GCAGAGGACC TGCAGGAGAA GTGCACTGAG 
CTGAACCAGG CATGGAGCAG CCTGGGCAAG CGTGCCGACC AGCGCAAGGC CAAGCTGGGC 
GACTCCCACG ACCTGCAGCG CTTCCTCAGC GACTTCCGGG ACCTCATGTC TTGGATCAAT 
GGAATCCGAG GTCTGGTGTC CTCAGACGAG CTAGCCAAGG ATGTCACCGG AGCTGAGGCC 
CTGCTGGAGC GACACCAGGA ACACCGGACA GAAATTGATG CCAGAGCTGG CACTTTCCAG 
GCATTCGAGC AGTTTGGGCA GCAGCTGTTG GCTCATGGGC ACTACGCCAG CCCCGAGATC 
AAGGAGAAGC TCGATGTTCT AGACCAGGAG CGTGCAGACC TTGAGAAGGC CTGGGTCCAG 
CGCAGGATGA TGCTGGATCA GTGCCTTGAA TTGCAGCTGT TCCATCGGGA TTGCGAGCAG 
GCCGAGAACT GGATGGCTGC CCGAGAGGCC TTTCTGAACA CAGAAGACAA AGGGGACTCG 
CTAGACAGTG TGGAGGCTCT GATCAAAAAA CACGAGGACT TTGATAAAGC AATTAATGTC 
CAGGAGGAGA AGATTGCTGC CCTGCAGTCC TTTGCTGACC AGCTCATCGC TGGTGGCCAC 
TACGCCAAAG GCGACATCTC CAGCCGGCGC AATGAGGTTC TGGACAGGTG GCGCCGTCTA 
AAAGCTCAGA TGATTGAGAA GAGGTCAAAG CTGGGAGAAT CACAGACCCT CCAGCAGTTC 
AGCCGGGATG TAGACGAGAT TGAAGCTTGG ATCAGTGAAA AATTACAGAC TGCAAGTGAT 
GAGTCATATA AGGACCCCAC CAACATCCAG AGCAAGCATC AGAAGCACCA GGCCTTCGAG 
GCAGAACTGC ACGCCAATGC GGACCGGATC CGTGGGGTCA TCGACATGGG CAACTCCCTC 
ATTGACCGCG GGGCCTGCGC CGGCAGCGAG GATGCCGTCA AGGCCCGGCT GGCCGCCTTA 
GCTGACCAGT GGCAGTTCCT GGTGCAGAAG TCGGCTGAGA AGAGCCAGAA GCTGAAAGAA 
GCCAACAAGC AGCAGAACTT CAACACTGGC ATCAAGGACT TCGACTTCTG GCTCTCTGAG 
GTGGAAGCCC TGCTGGCCTC TGAGGATTAT GGCAAAGACC TGGCTTCTGT GAACAACTTG 
CTAAAAAAAC ATCAGCTGCT GGAGGCAGAT ATCTCTGCCC ACGAGGACCG CCTGAAGGAC 
CTCAACAGCC AGGCGGACAG CCTGATGACC AGCAGCGCCT TCGACACCTC CCAGGTGAAG 
GACAAGCGGG ACGCCATCAA CGGCCGTTTC CAGAAGATCA AGAGCATGGC CGCCTCGAGG 
CGAGCTAGGC TGAATGAGTC CCACCGCCTG CACCAGTTTT TCCGGGACAT GGATGATGAG 
GAGTCCTGGA TCAAGGAAAA GAAGCTGCTG GTGAGCTCAG AGGACTACGG CCGTGACCTG 
ACGGGCGTCC AGAACCTGAG GAAGAAGCAC AAACGGCTGG AAGCAGAGCT GGCTGCCCAC 
GAGCCGGCCA TTCAGGGTGT CCTGGACACT GGAAAGAAGC TGTCTGATGA CAACACCATC 
GGGAAGGAGG AGATTCAGCA GCGCCTGGCG CAGTTCGTGG AGCACTGGAA GGAGCTGAAG 
CAGCTGGCAG CTGCCCGGGG CCAGCGGCTA GAAGAATCTT TGGAATATCA GCAGTTTGTA 
GCCAACGTGG AGGAGGAGGA AGCCTGGATC AATGAGAAGA TGACCCTGGT GGCCAGTGAA 
GATTATGGAG ACACTCTTGC TGCCATCCAG GGCTTGCTGA AGAAACACGA AGCCTTCGAG 
ACGGACTTCA CTGTCCACAA GGACCGAGTG AATGATGTCT GCACCAACGG CCAGGACCTG 
GTTAAGAAGA ACAATCACCA TGAGGAGAAC ATCTCTTCCA AGATGAAGGG CTTGAACGGG 
AAAGTGTCAG ACTTGGAGAA GGCTGCCTCC CAGAGGAAGG CCAAGCTCGA CGAGAACTCG 
GCCTTCCTCC AGTTCAACTG GAAAGCCGAT GTGGTGGAGT CCTGGATCGG TGAAAAGGAG 
AACAGCTTGA AGACAGATGA TTATGGTCGA GATCTGTCTT CTGTCCAAAC TCTCCTCACC 
AAACAGGAAA CCTTCGATGC TGGCCTGCAG GCCTTCCAGC AGGAGGGCAT CGCCAACATC 
ACTGCCCTCA AAGATCAGCT GCTGGCCGCC AAGCACGTTC AGTCCAAGGC CATCGAGGCC 
CGCCATGCTG CCCTCATGAA GAGGTGGAGC CAGCTCCTGG CCAACTCGGC CACCCGGAAG 
AAGAAGCTGC TGGAGGCACA GAGTCACTTC CGCAAGGTGG AGGATCTCTT CCTGACCTTT 
GCTAAAAAGG CTTCAGCCTT CAACAGCTGG TTTGAAAACG CAGAGGAGGA CTTAACCGAC 
CCAGTGCGCT GCAACTCTCT GGAAGAGATC AAGGCCTTGA GAGAGGCCCA CGACGCCTTC 
CGCTCCTCGT TGAGCTCGGC CCAGGCCGAC TTCAACCAGC TGGCAGAGCT GGACCGCCAG 
ATCAAGAGCT TCCGCGTGGC CTCCAACCCC TACACCTGGT TCACCATGGA GGCCCTGGAG 
GAGACCTGGA GAAACCTACA GAAGATCATC AAGGAAAGGG AGCTGGAGCT GCAGAAGGAG 
CAGCGACGGC AGGAGGAGAA TGACAAGCTG CGCCAGGAGT TCGCCCAGCA TGCCAACGCC 
TTCCACCAGT GGATCCAGGA GACCAGGTCC TGCATGGTGG AAGAATCCGG GACCCTCGAA 
TCACAGCTTG AAGCTACCAA ACGCAAACAC CAGGAGATTC GAGCTATGAG AAGTCAGCTC 
AAGAAGATTG AAGACCTGGG GGCGGCCATG GAGGAAGCGC TGATCCTGGA CAACAAGTAC 
ACGGAGCACA GCACCGTGGG CCTGGCCCAG CAGTGGGACC AGCTCGACCA GTTGGGCATG 
CGCATGCAGC ACAACCTGGA GCAGCAGATC CAGGCCAGGA ACACGACCGG AGTGACCGAG 
GAGGCCCTCA AAGAATTCAG CATGATGTTC AAACACTTCG ACAAGGACAA GTCTGGCCGG 
CTGAACCACC AGGAGTTCAA ATCCTGTCTG CGCTCCCTGG GCTATGACCT GCCCATGGTG 
GAGGAAGGGG AGCCCGACCC TGAGTTCGAG GCCATCCTGG ACACTGTGGA TCCTAACAGG 
GACGGCCACG TCTCCCTGCA AGAGTACATG GCCTTTATGA TCAGCCGTGA GACCGAGAAC 
GTCAAGTCCA GCGAGGAGAT TGAGAGCGCC TTCCGGGCCC TGAGCTCTGA AGGGAAGCCT 
TATGTGACCA AGGAGGAGCT GTACCAGAAC CTGACTCGGG AACAAGCCGA CTACTGCGTC 
TCCCACATGA AGCCCTACGT GGACGGCAAG GGCCGCGAAC TCCCCACTGC TTTTGACTAC 
GTCGAGTTCA CCCGCTCGCT CTTCGTGAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_024999028.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854796.1 Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa54548
Clone ID Related Accession (Same CDS sequence)	XM_024999028.1
Accession Version	XM_024999028.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7482bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT 
GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT 
CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC 
AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG 
AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC 
CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG 
AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT 
GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC 
CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC 
CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC 
CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT 
AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT 
GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG 
GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC 
ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC 
TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG 
AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG 
GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA 
GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG 
AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG 
CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG 
GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA 
ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG 
AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT 
GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT 
TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG 
CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT 
GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG 
AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG 
CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT 
CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC 
GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA 
GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG 
GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT 
CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG 
AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC 
AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC 
GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC 
CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC 
GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA 
GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC 
GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC 
AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC 
GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC 
AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGACGC GCGTAACTAA GGAGGCTGGC 
AGCGTGTCTC TGCGTATGAA ACAGGTGGAA GAACTGTACC ATTCTCTGCT GGAACTGGGA 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG 
AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC 
GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC 
AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA 
GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC 
AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG 
CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA 
GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC 
TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG 
GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC 
CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA 
TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC 
CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT 
CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG 
TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC 
GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG 
ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG 
GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG 
ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC 
GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG 
ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA 
GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG 
GACCCCACCA ACATCCAGAG CAAGCATCAG AAGCACCAGG CCTTCGAGGC AGAACTGCAC 
GCCAATGCGG ACCGGATCCG TGGGGTCATC GACATGGGCA ACTCCCTCAT TGACCGCGGG 
GCCTGCGCCG GCAGCGAGGA TGCCGTCAAG GCCCGGCTGG CCGCCTTAGC TGACCAGTGG 
CAGTTCCTGG TGCAGAAGTC GGCTGAGAAG AGCCAGAAGC TGAAAGAAGC CAACAAGCAG 
CAGAACTTCA ACACTGGCAT CAAGGACTTC GACTTCTGGC TCTCTGAGGT GGAAGCCCTG 
CTGGCCTCTG AGGATTATGG CAAAGACCTG GCTTCTGTGA ACAACTTGCT AAAAAAACAT 
CAGCTGCTGG AGGCAGATAT CTCTGCCCAC GAGGACCGCC TGAAGGACCT CAACAGCCAG 
GCGGACAGCC TGATGACCAG CAGCGCCTTC GACACCTCCC AGGTGAAGGA CAAGCGGGAC 
GCCATCAACG GCCGTTTCCA GAAGATCAAG AGCATGGCCG CCTCGAGGCG AGCTAGGCTG 
AATGAGTCCC ACCGCCTGCA CCAGTTTTTC CGGGACATGG ATGATGAGGA GTCCTGGATC 
AAGGAAAAGA AGCTGCTGGT GAGCTCAGAG GACTACGGCC GTGACCTGAC GGGCGTCCAG 
AACCTGAGGA AGAAGCACAA ACGGCTGGAA GCAGAGCTGG CTGCCCACGA GCCGGCCATT 
CAGGGTGTCC TGGACACTGG AAAGAAGCTG TCTGATGACA ACACCATCGG GAAGGAGGAG 
ATTCAGCAGC GCCTGGCGCA GTTCGTGGAG CACTGGAAGG AGCTGAAGCA GCTGGCAGCT 
GCCCGGGGCC AGCGGCTAGA AGAATCTTTG GAATATCAGC AGTTTGTAGC CAACGTGGAG 
GAGGAGGAAG CCTGGATCAA TGAGAAGATG ACCCTGGTGG CCAGTGAAGA TTATGGAGAC 
ACTCTTGCTG CCATCCAGGG CTTGCTGAAG AAACACGAAG CCTTCGAGAC GGACTTCACT 
GTCCACAAGG ACCGAGTGAA TGATGTCTGC ACCAACGGCC AGGACCTGGT TAAGAAGAAC 
AATCACCATG AGGAGAACAT CTCTTCCAAG ATGAAGGGCT TGAACGGGAA AGTGTCAGAC 
TTGGAGAAGG CTGCCTCCCA GAGGAAGGCC AAGCTCGACG AGAACTCGGC CTTCCTCCAG 
TTCAACTGGA AAGCCGATGT GGTGGAGTCC TGGATCGGTG AAAAGGAGAA CAGCTTGAAG 
ACAGATGATT ATGGTCGAGA TCTGTCTTCT GTCCAAACTC TCCTCACCAA ACAGGAAACC 
TTCGATGCTG GCCTGCAGGC CTTCCAGCAG GAGGGCATCG CCAACATCAC TGCCCTCAAA 
GATCAGCTGC TGGCCGCCAA GCACGTTCAG TCCAAGGCCA TCGAGGCCCG CCATGCTGCC 
CTCATGAAGA GGTGGAGCCA GCTCCTGGCC AACTCGGCCA CCCGGAAGAA GAAGCTGCTG 
GAGGCACAGA GTCACTTCCG CAAGGTGGAG GATCTCTTCC TGACCTTTGC TAAAAAGGCT 
TCAGCCTTCA ACAGCTGGTT TGAAAACGCA GAGGAGGACT TAACCGACCC AGTGCGCTGC 
AACTCTCTGG AAGAGATCAA GGCCTTGAGA GAGGCCCACG ACGCCTTCCG CTCCTCGTTG 
AGCTCGGCCC AGGCCGACTT CAACCAGCTG GCAGAGCTGG ACCGCCAGAT CAAGAGCTTC 
CGCGTGGCCT CCAACCCCTA CACCTGGTTC ACCATGGAGG CCCTGGAGGA GACCTGGAGA 
AACCTACAGA AGATCATCAA GGAAAGGGAG CTGGAGCTGC AGAAGGAGCA GCGACGGCAG 
GAGGAGAATG ACAAGCTGCG CCAGGAGTTC GCCCAGCATG CCAACGCCTT CCACCAGTGG 
ATCCAGGAGA CCAGAACGTA CCTCCTCGAT GGCATAGCGT ATCGTCGGGT CATTCGTGTC 
TATCAGTATG AAGTTGGGGA TGATCTGTCT GGAAGGTCCT GCATGGTGGA AGAATCCGGG 
ACCCTCGAAT CACAGCTTGA AGCTACCAAA CGCAAACACC AGGAGATTCG AGCTATGAGA 
AGTCAGCTCA AGAAGATTGA AGACCTGGGG GCGGCCATGG AGGAAGCGCT GATCCTGGAC 
AACAAGTACA CGGAGCACAG CACCGTGGGC CTGGCCCAGC AGTGGGACCA GCTCGACCAG 
TTGGGCATGC GCATGCAGCA CAACCTGGAG CAGCAGATCC AGGCCAGGAA CACGACCGGA 
GTGACCGAGG AGGCCCTCAA AGAATTCAGC ATGATGTTCA AACACTTCGA CAAGGACAAG 
TCTGGCCGGC TGAACCACCA GGAGTTCAAA TCCTGTCTGC GCTCCCTGGG CTATGACCTG 
CCCATGGTGG AGGAAGGGGA GCCCGACCCT GAGTTCGAGG CCATCCTGGA CACTGTGGAT 
CCTAACAGGG ACGGCCACGT CTCCCTGCAA GAGTACATGG CCTTTATGAT CAGCCGTGAG 
ACCGAGAACG TCAAGTCCAG CGAGGAGATT GAGAGCGCCT TCCGGGCCCT GAGCTCTGAA 
GGGAAGCCTT ATGTGACCAA GGAGGAGCTG TACCAGAACC TGACTCGGGA ACAAGCCGAC 
TACTGCGTCT CCCACATGAA GCCCTACGTG GACGGCAAGG GCCGCGAACT CCCCACTGCT 
TTTGACTACG TCGAGTTCAC CCGCTCGCTC TTCGTGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854796.1
CDS	4..7485
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLHQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMYRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVDHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWESLKSKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRVTKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGAMPRDESDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKEKLDVLDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQSFADQLIAGGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQSKHQKHQAFEAELHANADRIRGVIDMGNSLIDRGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDAINGRFQKIKSMAASRRARLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLVKKNNHHEENISSKMKGLNGKVSDLEKAASQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHAALMKRWSQLLANSATRKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGIAYRRVIRVYQYEVGDDLSGRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_024999028.1
Organism	Bos taurus(cattle)
Definition	Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999028.1

ORF Insert Sequence:

ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT 
GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT 
CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC 
AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG 
AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC 
CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG 
AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT 
GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC 
CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC 
CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC 
CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT 
AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT 
GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG 
GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC 
ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC 
TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG 
AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG 
GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA 
GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG 
AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG 
CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG 
GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA 
ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG 
AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT 
GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT 
TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG 
CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT 
GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG 
AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG 
CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT 
CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC 
GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA 
GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG 
GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT 
CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG 
AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC 
AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC 
GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC 
CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC 
GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA 
GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC 
GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC 
AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC 
GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC 
AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGACGC GCGTAACTAA GGAGGCTGGC 
AGCGTGTCTC TGCGTATGAA ACAGGTGGAA GAACTGTACC ATTCTCTGCT GGAACTGGGA 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG 
AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC 
GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC 
AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA 
GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC 
AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG 
CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA 
GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC 
TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG 
GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC 
CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA 
TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC 
CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT 
CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG 
TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC 
GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG 
ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG 
GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG 
ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC 
GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG 
ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA 
GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG 
GACCCCACCA ACATCCAGAG CAAGCATCAG AAGCACCAGG CCTTCGAGGC AGAACTGCAC 
GCCAATGCGG ACCGGATCCG TGGGGTCATC GACATGGGCA ACTCCCTCAT TGACCGCGGG 
GCCTGCGCCG GCAGCGAGGA TGCCGTCAAG GCCCGGCTGG CCGCCTTAGC TGACCAGTGG 
CAGTTCCTGG TGCAGAAGTC GGCTGAGAAG AGCCAGAAGC TGAAAGAAGC CAACAAGCAG 
CAGAACTTCA ACACTGGCAT CAAGGACTTC GACTTCTGGC TCTCTGAGGT GGAAGCCCTG 
CTGGCCTCTG AGGATTATGG CAAAGACCTG GCTTCTGTGA ACAACTTGCT AAAAAAACAT 
CAGCTGCTGG AGGCAGATAT CTCTGCCCAC GAGGACCGCC TGAAGGACCT CAACAGCCAG 
GCGGACAGCC TGATGACCAG CAGCGCCTTC GACACCTCCC AGGTGAAGGA CAAGCGGGAC 
GCCATCAACG GCCGTTTCCA GAAGATCAAG AGCATGGCCG CCTCGAGGCG AGCTAGGCTG 
AATGAGTCCC ACCGCCTGCA CCAGTTTTTC CGGGACATGG ATGATGAGGA GTCCTGGATC 
AAGGAAAAGA AGCTGCTGGT GAGCTCAGAG GACTACGGCC GTGACCTGAC GGGCGTCCAG 
AACCTGAGGA AGAAGCACAA ACGGCTGGAA GCAGAGCTGG CTGCCCACGA GCCGGCCATT 
CAGGGTGTCC TGGACACTGG AAAGAAGCTG TCTGATGACA ACACCATCGG GAAGGAGGAG 
ATTCAGCAGC GCCTGGCGCA GTTCGTGGAG CACTGGAAGG AGCTGAAGCA GCTGGCAGCT 
GCCCGGGGCC AGCGGCTAGA AGAATCTTTG GAATATCAGC AGTTTGTAGC CAACGTGGAG 
GAGGAGGAAG CCTGGATCAA TGAGAAGATG ACCCTGGTGG CCAGTGAAGA TTATGGAGAC 
ACTCTTGCTG CCATCCAGGG CTTGCTGAAG AAACACGAAG CCTTCGAGAC GGACTTCACT 
GTCCACAAGG ACCGAGTGAA TGATGTCTGC ACCAACGGCC AGGACCTGGT TAAGAAGAAC 
AATCACCATG AGGAGAACAT CTCTTCCAAG ATGAAGGGCT TGAACGGGAA AGTGTCAGAC 
TTGGAGAAGG CTGCCTCCCA GAGGAAGGCC AAGCTCGACG AGAACTCGGC CTTCCTCCAG 
TTCAACTGGA AAGCCGATGT GGTGGAGTCC TGGATCGGTG AAAAGGAGAA CAGCTTGAAG 
ACAGATGATT ATGGTCGAGA TCTGTCTTCT GTCCAAACTC TCCTCACCAA ACAGGAAACC 
TTCGATGCTG GCCTGCAGGC CTTCCAGCAG GAGGGCATCG CCAACATCAC TGCCCTCAAA 
GATCAGCTGC TGGCCGCCAA GCACGTTCAG TCCAAGGCCA TCGAGGCCCG CCATGCTGCC 
CTCATGAAGA GGTGGAGCCA GCTCCTGGCC AACTCGGCCA CCCGGAAGAA GAAGCTGCTG 
GAGGCACAGA GTCACTTCCG CAAGGTGGAG GATCTCTTCC TGACCTTTGC TAAAAAGGCT 
TCAGCCTTCA ACAGCTGGTT TGAAAACGCA GAGGAGGACT TAACCGACCC AGTGCGCTGC 
AACTCTCTGG AAGAGATCAA GGCCTTGAGA GAGGCCCACG ACGCCTTCCG CTCCTCGTTG 
AGCTCGGCCC AGGCCGACTT CAACCAGCTG GCAGAGCTGG ACCGCCAGAT CAAGAGCTTC 
CGCGTGGCCT CCAACCCCTA CACCTGGTTC ACCATGGAGG CCCTGGAGGA GACCTGGAGA 
AACCTACAGA AGATCATCAA GGAAAGGGAG CTGGAGCTGC AGAAGGAGCA GCGACGGCAG 
GAGGAGAATG ACAAGCTGCG CCAGGAGTTC GCCCAGCATG CCAACGCCTT CCACCAGTGG 
ATCCAGGAGA CCAGAACGTA CCTCCTCGAT GGCATAGCGT ATCGTCGGGT CATTCGTGTC 
TATCAGTATG AAGTTGGGGA TGATCTGTCT GGAAGGTCCT GCATGGTGGA AGAATCCGGG 
ACCCTCGAAT CACAGCTTGA AGCTACCAAA CGCAAACACC AGGAGATTCG AGCTATGAGA 
AGTCAGCTCA AGAAGATTGA AGACCTGGGG GCGGCCATGG AGGAAGCGCT GATCCTGGAC 
AACAAGTACA CGGAGCACAG CACCGTGGGC CTGGCCCAGC AGTGGGACCA GCTCGACCAG 
TTGGGCATGC GCATGCAGCA CAACCTGGAG CAGCAGATCC AGGCCAGGAA CACGACCGGA 
GTGACCGAGG AGGCCCTCAA AGAATTCAGC ATGATGTTCA AACACTTCGA CAAGGACAAG 
TCTGGCCGGC TGAACCACCA GGAGTTCAAA TCCTGTCTGC GCTCCCTGGG CTATGACCTG 
CCCATGGTGG AGGAAGGGGA GCCCGACCCT GAGTTCGAGG CCATCCTGGA CACTGTGGAT 
CCTAACAGGG ACGGCCACGT CTCCCTGCAA GAGTACATGG CCTTTATGAT CAGCCGTGAG 
ACCGAGAACG TCAAGTCCAG CGAGGAGATT GAGAGCGCCT TCCGGGCCCT GAGCTCTGAA 
GGGAAGCCTT ATGTGACCAA GGAGGAGCTG TACCAGAACC TGACTCGGGA ACAAGCCGAC 
TACTGCGTCT CCCACATGAA GCCCTACGTG GACGGCAAGG GCCGCGAACT CCCCACTGCT 
TTTGACTACG TCGAGTTCAC CCGCTCGCTC TTCGTGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_024999030.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854798.1 Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa54549
Clone ID Related Accession (Same CDS sequence)	XM_024999030.1
Accession Version	XM_024999030.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7449bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT 
GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT 
CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC 
AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG 
AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC 
CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG 
AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT 
GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC 
CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC 
CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC 
CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT 
AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT 
GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG 
GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC 
ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC 
TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG 
AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG 
GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA 
GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG 
AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG 
CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG 
GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA 
ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG 
AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT 
GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT 
TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG 
CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT 
GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG 
AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG 
CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT 
CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC 
GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA 
GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG 
GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT 
CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG 
AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC 
AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC 
GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC 
CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC 
GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA 
GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC 
GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC 
AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC 
GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC 
AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGACGC GCGTAACTAA GGAGGCTGGC 
AGCGTGTCTC TGCGTATGAA ACAGGTGGAA GAACTGTACC ATTCTCTGCT GGAACTGGGA 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG 
AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC 
GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC 
AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA 
GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC 
AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGG AGCTGAACGA GCGCTGGCGG 
TCCCTGCAGC AGCTGGCTGA GGAGCGGAGC CAACTCTTGG GCAGTGCCCA TGAAGTGCAG 
CGGTTCCACA GAGATGCTGA CGAAACCAAA GAGTGGATCG AAGAGAAGAA CCAGGCTCTA 
AACACAGACA ACTACGGCCA CGACCTGGCC AGTGTCCAGG CCCTGCAGCG CAAGCACGAG 
GGCTTCGAGA GGGACCTCGC AGCCCTTGGT GACAAGGTGA ACTCGCTCGG GGAGACAGCC 
GAGCGCCTGA TCCAGTCCCA CCCCGAGTCG GCAGAGGACC TGCAGGAGAA GTGCACTGAG 
CTGAACCAGG CATGGAGCAG CCTGGGCAAG CGTGCCGACC AGCGCAAGGC CAAGCTGGGC 
GACTCCCACG ACCTGCAGCG CTTCCTCAGC GACTTCCGGG ACCTCATGTC TTGGATCAAT 
GGAATCCGAG GTCTGGTGTC CTCAGACGAG CTAGCCAAGG ATGTCACCGG AGCTGAGGCC 
CTGCTGGAGC GACACCAGGA ACACCGGACA GAAATTGATG CCAGAGCTGG CACTTTCCAG 
GCATTCGAGC AGTTTGGGCA GCAGCTGTTG GCTCATGGGC ACTACGCCAG CCCCGAGATC 
AAGGAGAAGC TCGATGTTCT AGACCAGGAG CGTGCAGACC TTGAGAAGGC CTGGGTCCAG 
CGCAGGATGA TGCTGGATCA GTGCCTTGAA TTGCAGCTGT TCCATCGGGA TTGCGAGCAG 
GCCGAGAACT GGATGGCTGC CCGAGAGGCC TTTCTGAACA CAGAAGACAA AGGGGACTCG 
CTAGACAGTG TGGAGGCTCT GATCAAAAAA CACGAGGACT TTGATAAAGC AATTAATGTC 
CAGGAGGAGA AGATTGCTGC CCTGCAGTCC TTTGCTGACC AGCTCATCGC TGGTGGCCAC 
TACGCCAAAG GCGACATCTC CAGCCGGCGC AATGAGGTTC TGGACAGGTG GCGCCGTCTA 
AAAGCTCAGA TGATTGAGAA GAGGTCAAAG CTGGGAGAAT CACAGACCCT CCAGCAGTTC 
AGCCGGGATG TAGACGAGAT TGAAGCTTGG ATCAGTGAAA AATTACAGAC TGCAAGTGAT 
GAGTCATATA AGGACCCCAC CAACATCCAG CTTTCCAAGC TGCTGAGCAA GCATCAGAAG 
CACCAGGCCT TCGAGGCAGA ACTGCACGCC AATGCGGACC GGATCCGTGG GGTCATCGAC 
ATGGGCAACT CCCTCATTGA CCGCGGGGCC TGCGCCGGCA GCGAGGATGC CGTCAAGGCC 
CGGCTGGCCG CCTTAGCTGA CCAGTGGCAG TTCCTGGTGC AGAAGTCGGC TGAGAAGAGC 
CAGAAGCTGA AAGAAGCCAA CAAGCAGCAG AACTTCAACA CTGGCATCAA GGACTTCGAC 
TTCTGGCTCT CTGAGGTGGA AGCCCTGCTG GCCTCTGAGG ATTATGGCAA AGACCTGGCT 
TCTGTGAACA ACTTGCTAAA AAAACATCAG CTGCTGGAGG CAGATATCTC TGCCCACGAG 
GACCGCCTGA AGGACCTCAA CAGCCAGGCG GACAGCCTGA TGACCAGCAG CGCCTTCGAC 
ACCTCCCAGG TGAAGGACAA GCGGGACGCC ATCAACGGCC GTTTCCAGAA GATCAAGAGC 
ATGGCCGCCT CGAGGCGAGC TAGGCTGAAT GAGTCCCACC GCCTGCACCA GTTTTTCCGG 
GACATGGATG ATGAGGAGTC CTGGATCAAG GAAAAGAAGC TGCTGGTGAG CTCAGAGGAC 
TACGGCCGTG ACCTGACGGG CGTCCAGAAC CTGAGGAAGA AGCACAAACG GCTGGAAGCA 
GAGCTGGCTG CCCACGAGCC GGCCATTCAG GGTGTCCTGG ACACTGGAAA GAAGCTGTCT 
GATGACAACA CCATCGGGAA GGAGGAGATT CAGCAGCGCC TGGCGCAGTT CGTGGAGCAC 
TGGAAGGAGC TGAAGCAGCT GGCAGCTGCC CGGGGCCAGC GGCTAGAAGA ATCTTTGGAA 
TATCAGCAGT TTGTAGCCAA CGTGGAGGAG GAGGAAGCCT GGATCAATGA GAAGATGACC 
CTGGTGGCCA GTGAAGATTA TGGAGACACT CTTGCTGCCA TCCAGGGCTT GCTGAAGAAA 
CACGAAGCCT TCGAGACGGA CTTCACTGTC CACAAGGACC GAGTGAATGA TGTCTGCACC 
AACGGCCAGG ACCTGGTTAA GAAGAACAAT CACCATGAGG AGAACATCTC TTCCAAGATG 
AAGGGCTTGA ACGGGAAAGT GTCAGACTTG GAGAAGGCTG CCTCCCAGAG GAAGGCCAAG 
CTCGACGAGA ACTCGGCCTT CCTCCAGTTC AACTGGAAAG CCGATGTGGT GGAGTCCTGG 
ATCGGTGAAA AGGAGAACAG CTTGAAGACA GATGATTATG GTCGAGATCT GTCTTCTGTC 
CAAACTCTCC TCACCAAACA GGAAACCTTC GATGCTGGCC TGCAGGCCTT CCAGCAGGAG 
GGCATCGCCA ACATCACTGC CCTCAAAGAT CAGCTGCTGG CCGCCAAGCA CGTTCAGTCC 
AAGGCCATCG AGGCCCGCCA TGCTGCCCTC ATGAAGAGGT GGAGCCAGCT CCTGGCCAAC 
TCGGCCACCC GGAAGAAGAA GCTGCTGGAG GCACAGAGTC ACTTCCGCAA GGTGGAGGAT 
CTCTTCCTGA CCTTTGCTAA AAAGGCTTCA GCCTTCAACA GCTGGTTTGA AAACGCAGAG 
GAGGACTTAA CCGACCCAGT GCGCTGCAAC TCTCTGGAAG AGATCAAGGC CTTGAGAGAG 
GCCCACGACG CCTTCCGCTC CTCGTTGAGC TCGGCCCAGG CCGACTTCAA CCAGCTGGCA 
GAGCTGGACC GCCAGATCAA GAGCTTCCGC GTGGCCTCCA ACCCCTACAC CTGGTTCACC 
ATGGAGGCCC TGGAGGAGAC CTGGAGAAAC CTACAGAAGA TCATCAAGGA AAGGGAGCTG 
GAGCTGCAGA AGGAGCAGCG ACGGCAGGAG GAGAATGACA AGCTGCGCCA GGAGTTCGCC 
CAGCATGCCA ACGCCTTCCA CCAGTGGATC CAGGAGACCA GAACGTACCT CCTCGATGGC 
ATAGCGTATC GTCGGGTCAT TCGTGTCTAT CAGTATGAAG TTGGGGATGA TCTGTCTGGA 
AGGTCCTGCA TGGTGGAAGA ATCCGGGACC CTCGAATCAC AGCTTGAAGC TACCAAACGC 
AAACACCAGG AGATTCGAGC TATGAGAAGT CAGCTCAAGA AGATTGAAGA CCTGGGGGCG 
GCCATGGAGG AAGCGCTGAT CCTGGACAAC AAGTACACGG AGCACAGCAC CGTGGGCCTG 
GCCCAGCAGT GGGACCAGCT CGACCAGTTG GGCATGCGCA TGCAGCACAA CCTGGAGCAG 
CAGATCCAGG CCAGGAACAC GACCGGAGTG ACCGAGGAGG CCCTCAAAGA ATTCAGCATG 
ATGTTCAAAC ACTTCGACAA GGACAAGTCT GGCCGGCTGA ACCACCAGGA GTTCAAATCC 
TGTCTGCGCT CCCTGGGCTA TGACCTGCCC ATGGTGGAGG AAGGGGAGCC CGACCCTGAG 
TTCGAGGCCA TCCTGGACAC TGTGGATCCT AACAGGGACG GCCACGTCTC CCTGCAAGAG 
TACATGGCCT TTATGATCAG CCGTGAGACC GAGAACGTCA AGTCCAGCGA GGAGATTGAG 
AGCGCCTTCC GGGCCCTGAG CTCTGAAGGG AAGCCTTATG TGACCAAGGA GGAGCTGTAC 
CAGAACCTGA CTCGGGAACA AGCCGACTAC TGCGTCTCCC ACATGAAGCC CTACGTGGAC 
GGCAAGGGCC GCGAACTCCC CACTGCTTTT GACTACGTCG AGTTCACCCG CTCGCTCTTC 
GTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854798.1
CDS	4..7452
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLHQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMYRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVDHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWESLKSKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRVTKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGAMPRDESDSKTASPWKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKEKLDVLDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQSFADQLIAGGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDMGNSLIDRGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDAINGRFQKIKSMAASRRARLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLVKKNNHHEENISSKMKGLNGKVSDLEKAASQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHAALMKRWSQLLANSATRKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGIAYRRVIRVYQYEVGDDLSGRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_024999030.1
Organism	Bos taurus(cattle)
Definition	Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999030.1

ORF Insert Sequence:

ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT 
GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT 
CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC 
AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG 
AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC 
CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG 
AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT 
GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC 
CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC 
CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC 
CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT 
AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT 
GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG 
GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC 
ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC 
TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG 
AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG 
GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA 
GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG 
AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG 
CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG 
GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA 
ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG 
AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT 
GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT 
TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG 
CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT 
GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG 
AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG 
CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT 
CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC 
GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA 
GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG 
GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT 
CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG 
AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC 
AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC 
GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC 
CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC 
GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA 
GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC 
GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC 
AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC 
GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC 
AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGACGC GCGTAACTAA GGAGGCTGGC 
AGCGTGTCTC TGCGTATGAA ACAGGTGGAA GAACTGTACC ATTCTCTGCT GGAACTGGGA 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG 
AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC 
GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC 
AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA 
GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC 
AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGG AGCTGAACGA GCGCTGGCGG 
TCCCTGCAGC AGCTGGCTGA GGAGCGGAGC CAACTCTTGG GCAGTGCCCA TGAAGTGCAG 
CGGTTCCACA GAGATGCTGA CGAAACCAAA GAGTGGATCG AAGAGAAGAA CCAGGCTCTA 
AACACAGACA ACTACGGCCA CGACCTGGCC AGTGTCCAGG CCCTGCAGCG CAAGCACGAG 
GGCTTCGAGA GGGACCTCGC AGCCCTTGGT GACAAGGTGA ACTCGCTCGG GGAGACAGCC 
GAGCGCCTGA TCCAGTCCCA CCCCGAGTCG GCAGAGGACC TGCAGGAGAA GTGCACTGAG 
CTGAACCAGG CATGGAGCAG CCTGGGCAAG CGTGCCGACC AGCGCAAGGC CAAGCTGGGC 
GACTCCCACG ACCTGCAGCG CTTCCTCAGC GACTTCCGGG ACCTCATGTC TTGGATCAAT 
GGAATCCGAG GTCTGGTGTC CTCAGACGAG CTAGCCAAGG ATGTCACCGG AGCTGAGGCC 
CTGCTGGAGC GACACCAGGA ACACCGGACA GAAATTGATG CCAGAGCTGG CACTTTCCAG 
GCATTCGAGC AGTTTGGGCA GCAGCTGTTG GCTCATGGGC ACTACGCCAG CCCCGAGATC 
AAGGAGAAGC TCGATGTTCT AGACCAGGAG CGTGCAGACC TTGAGAAGGC CTGGGTCCAG 
CGCAGGATGA TGCTGGATCA GTGCCTTGAA TTGCAGCTGT TCCATCGGGA TTGCGAGCAG 
GCCGAGAACT GGATGGCTGC CCGAGAGGCC TTTCTGAACA CAGAAGACAA AGGGGACTCG 
CTAGACAGTG TGGAGGCTCT GATCAAAAAA CACGAGGACT TTGATAAAGC AATTAATGTC 
CAGGAGGAGA AGATTGCTGC CCTGCAGTCC TTTGCTGACC AGCTCATCGC TGGTGGCCAC 
TACGCCAAAG GCGACATCTC CAGCCGGCGC AATGAGGTTC TGGACAGGTG GCGCCGTCTA 
AAAGCTCAGA TGATTGAGAA GAGGTCAAAG CTGGGAGAAT CACAGACCCT CCAGCAGTTC 
AGCCGGGATG TAGACGAGAT TGAAGCTTGG ATCAGTGAAA AATTACAGAC TGCAAGTGAT 
GAGTCATATA AGGACCCCAC CAACATCCAG CTTTCCAAGC TGCTGAGCAA GCATCAGAAG 
CACCAGGCCT TCGAGGCAGA ACTGCACGCC AATGCGGACC GGATCCGTGG GGTCATCGAC 
ATGGGCAACT CCCTCATTGA CCGCGGGGCC TGCGCCGGCA GCGAGGATGC CGTCAAGGCC 
CGGCTGGCCG CCTTAGCTGA CCAGTGGCAG TTCCTGGTGC AGAAGTCGGC TGAGAAGAGC 
CAGAAGCTGA AAGAAGCCAA CAAGCAGCAG AACTTCAACA CTGGCATCAA GGACTTCGAC 
TTCTGGCTCT CTGAGGTGGA AGCCCTGCTG GCCTCTGAGG ATTATGGCAA AGACCTGGCT 
TCTGTGAACA ACTTGCTAAA AAAACATCAG CTGCTGGAGG CAGATATCTC TGCCCACGAG 
GACCGCCTGA AGGACCTCAA CAGCCAGGCG GACAGCCTGA TGACCAGCAG CGCCTTCGAC 
ACCTCCCAGG TGAAGGACAA GCGGGACGCC ATCAACGGCC GTTTCCAGAA GATCAAGAGC 
ATGGCCGCCT CGAGGCGAGC TAGGCTGAAT GAGTCCCACC GCCTGCACCA GTTTTTCCGG 
GACATGGATG ATGAGGAGTC CTGGATCAAG GAAAAGAAGC TGCTGGTGAG CTCAGAGGAC 
TACGGCCGTG ACCTGACGGG CGTCCAGAAC CTGAGGAAGA AGCACAAACG GCTGGAAGCA 
GAGCTGGCTG CCCACGAGCC GGCCATTCAG GGTGTCCTGG ACACTGGAAA GAAGCTGTCT 
GATGACAACA CCATCGGGAA GGAGGAGATT CAGCAGCGCC TGGCGCAGTT CGTGGAGCAC 
TGGAAGGAGC TGAAGCAGCT GGCAGCTGCC CGGGGCCAGC GGCTAGAAGA ATCTTTGGAA 
TATCAGCAGT TTGTAGCCAA CGTGGAGGAG GAGGAAGCCT GGATCAATGA GAAGATGACC 
CTGGTGGCCA GTGAAGATTA TGGAGACACT CTTGCTGCCA TCCAGGGCTT GCTGAAGAAA 
CACGAAGCCT TCGAGACGGA CTTCACTGTC CACAAGGACC GAGTGAATGA TGTCTGCACC 
AACGGCCAGG ACCTGGTTAA GAAGAACAAT CACCATGAGG AGAACATCTC TTCCAAGATG 
AAGGGCTTGA ACGGGAAAGT GTCAGACTTG GAGAAGGCTG CCTCCCAGAG GAAGGCCAAG 
CTCGACGAGA ACTCGGCCTT CCTCCAGTTC AACTGGAAAG CCGATGTGGT GGAGTCCTGG 
ATCGGTGAAA AGGAGAACAG CTTGAAGACA GATGATTATG GTCGAGATCT GTCTTCTGTC 
CAAACTCTCC TCACCAAACA GGAAACCTTC GATGCTGGCC TGCAGGCCTT CCAGCAGGAG 
GGCATCGCCA ACATCACTGC CCTCAAAGAT CAGCTGCTGG CCGCCAAGCA CGTTCAGTCC 
AAGGCCATCG AGGCCCGCCA TGCTGCCCTC ATGAAGAGGT GGAGCCAGCT CCTGGCCAAC 
TCGGCCACCC GGAAGAAGAA GCTGCTGGAG GCACAGAGTC ACTTCCGCAA GGTGGAGGAT 
CTCTTCCTGA CCTTTGCTAA AAAGGCTTCA GCCTTCAACA GCTGGTTTGA AAACGCAGAG 
GAGGACTTAA CCGACCCAGT GCGCTGCAAC TCTCTGGAAG AGATCAAGGC CTTGAGAGAG 
GCCCACGACG CCTTCCGCTC CTCGTTGAGC TCGGCCCAGG CCGACTTCAA CCAGCTGGCA 
GAGCTGGACC GCCAGATCAA GAGCTTCCGC GTGGCCTCCA ACCCCTACAC CTGGTTCACC 
ATGGAGGCCC TGGAGGAGAC CTGGAGAAAC CTACAGAAGA TCATCAAGGA AAGGGAGCTG 
GAGCTGCAGA AGGAGCAGCG ACGGCAGGAG GAGAATGACA AGCTGCGCCA GGAGTTCGCC 
CAGCATGCCA ACGCCTTCCA CCAGTGGATC CAGGAGACCA GAACGTACCT CCTCGATGGC 
ATAGCGTATC GTCGGGTCAT TCGTGTCTAT CAGTATGAAG TTGGGGATGA TCTGTCTGGA 
AGGTCCTGCA TGGTGGAAGA ATCCGGGACC CTCGAATCAC AGCTTGAAGC TACCAAACGC 
AAACACCAGG AGATTCGAGC TATGAGAAGT CAGCTCAAGA AGATTGAAGA CCTGGGGGCG 
GCCATGGAGG AAGCGCTGAT CCTGGACAAC AAGTACACGG AGCACAGCAC CGTGGGCCTG 
GCCCAGCAGT GGGACCAGCT CGACCAGTTG GGCATGCGCA TGCAGCACAA CCTGGAGCAG 
CAGATCCAGG CCAGGAACAC GACCGGAGTG ACCGAGGAGG CCCTCAAAGA ATTCAGCATG 
ATGTTCAAAC ACTTCGACAA GGACAAGTCT GGCCGGCTGA ACCACCAGGA GTTCAAATCC 
TGTCTGCGCT CCCTGGGCTA TGACCTGCCC ATGGTGGAGG AAGGGGAGCC CGACCCTGAG 
TTCGAGGCCA TCCTGGACAC TGTGGATCCT AACAGGGACG GCCACGTCTC CCTGCAAGAG 
TACATGGCCT TTATGATCAG CCGTGAGACC GAGAACGTCA AGTCCAGCGA GGAGATTGAG 
AGCGCCTTCC GGGCCCTGAG CTCTGAAGGG AAGCCTTATG TGACCAAGGA GGAGCTGTAC 
CAGAACCTGA CTCGGGAACA AGCCGACTAC TGCGTCTCCC ACATGAAGCC CTACGTGGAC 
GGCAAGGGCC GCGAACTCCC CACTGCTTTT GACTACGTCG AGTTCACCCG CTCGCTCTTC 
GTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_024999032.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854800.1 Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa54550
Clone ID Related Accession (Same CDS sequence)	XM_024999032.1
Accession Version	XM_024999032.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7437bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT 
GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT 
CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC 
AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG 
AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC 
CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG 
AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT 
GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC 
CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC 
CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC 
CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT 
AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT 
GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG 
GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC 
ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC 
TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG 
AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG 
GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA 
GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG 
AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG 
CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG 
GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA 
ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG 
AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT 
GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT 
TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG 
CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT 
GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG 
AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG 
CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT 
CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC 
GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA 
GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG 
GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT 
CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG 
AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC 
AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC 
GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC 
CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC 
GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA 
GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC 
GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC 
AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC 
GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC 
AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGTACC ATTCTCTGCT GGAACTGGGA 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG 
AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC 
GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC 
AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA 
GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC 
AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG 
CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA 
GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC 
TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG 
GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC 
CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA 
TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC 
CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT 
CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG 
TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC 
GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG 
ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG 
GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG 
ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC 
GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG 
ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA 
GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG 
GACCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ATCAGAAGCA CCAGGCCTTC 
GAGGCAGAAC TGCACGCCAA TGCGGACCGG ATCCGTGGGG TCATCGACAT GGGCAACTCC 
CTCATTGACC GCGGGGCCTG CGCCGGCAGC GAGGATGCCG TCAAGGCCCG GCTGGCCGCC 
TTAGCTGACC AGTGGCAGTT CCTGGTGCAG AAGTCGGCTG AGAAGAGCCA GAAGCTGAAA 
GAAGCCAACA AGCAGCAGAA CTTCAACACT GGCATCAAGG ACTTCGACTT CTGGCTCTCT 
GAGGTGGAAG CCCTGCTGGC CTCTGAGGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC 
TTGCTAAAAA AACATCAGCT GCTGGAGGCA GATATCTCTG CCCACGAGGA CCGCCTGAAG 
GACCTCAACA GCCAGGCGGA CAGCCTGATG ACCAGCAGCG CCTTCGACAC CTCCCAGGTG 
AAGGACAAGC GGGACGCCAT CAACGGCCGT TTCCAGAAGA TCAAGAGCAT GGCCGCCTCG 
AGGCGAGCTA GGCTGAATGA GTCCCACCGC CTGCACCAGT TTTTCCGGGA CATGGATGAT 
GAGGAGTCCT GGATCAAGGA AAAGAAGCTG CTGGTGAGCT CAGAGGACTA CGGCCGTGAC 
CTGACGGGCG TCCAGAACCT GAGGAAGAAG CACAAACGGC TGGAAGCAGA GCTGGCTGCC 
CACGAGCCGG CCATTCAGGG TGTCCTGGAC ACTGGAAAGA AGCTGTCTGA TGACAACACC 
ATCGGGAAGG AGGAGATTCA GCAGCGCCTG GCGCAGTTCG TGGAGCACTG GAAGGAGCTG 
AAGCAGCTGG CAGCTGCCCG GGGCCAGCGG CTAGAAGAAT CTTTGGAATA TCAGCAGTTT 
GTAGCCAACG TGGAGGAGGA GGAAGCCTGG ATCAATGAGA AGATGACCCT GGTGGCCAGT 
GAAGATTATG GAGACACTCT TGCTGCCATC CAGGGCTTGC TGAAGAAACA CGAAGCCTTC 
GAGACGGACT TCACTGTCCA CAAGGACCGA GTGAATGATG TCTGCACCAA CGGCCAGGAC 
CTGGTTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CCAAGATGAA GGGCTTGAAC 
GGGAAAGTGT CAGACTTGGA GAAGGCTGCC TCCCAGAGGA AGGCCAAGCT CGACGAGAAC 
TCGGCCTTCC TCCAGTTCAA CTGGAAAGCC GATGTGGTGG AGTCCTGGAT CGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGT CGAGATCTGT CTTCTGTCCA AACTCTCCTC 
ACCAAACAGG AAACCTTCGA TGCTGGCCTG CAGGCCTTCC AGCAGGAGGG CATCGCCAAC 
ATCACTGCCC TCAAAGATCA GCTGCTGGCC GCCAAGCACG TTCAGTCCAA GGCCATCGAG 
GCCCGCCATG CTGCCCTCAT GAAGAGGTGG AGCCAGCTCC TGGCCAACTC GGCCACCCGG 
AAGAAGAAGC TGCTGGAGGC ACAGAGTCAC TTCCGCAAGG TGGAGGATCT CTTCCTGACC 
TTTGCTAAAA AGGCTTCAGC CTTCAACAGC TGGTTTGAAA ACGCAGAGGA GGACTTAACC 
GACCCAGTGC GCTGCAACTC TCTGGAAGAG ATCAAGGCCT TGAGAGAGGC CCACGACGCC 
TTCCGCTCCT CGTTGAGCTC GGCCCAGGCC GACTTCAACC AGCTGGCAGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT GGCCTCCAAC CCCTACACCT GGTTCACCAT GGAGGCCCTG 
GAGGAGACCT GGAGAAACCT ACAGAAGATC ATCAAGGAAA GGGAGCTGGA GCTGCAGAAG 
GAGCAGCGAC GGCAGGAGGA GAATGACAAG CTGCGCCAGG AGTTCGCCCA GCATGCCAAC 
GCCTTCCACC AGTGGATCCA GGAGACCAGA ACGTACCTCC TCGATGGCAT AGCGTATCGT 
CGGGTCATTC GTGTCTATCA GTATGAAGTT GGGGATGATC TGTCTGGAAG GTCCTGCATG 
GTGGAAGAAT CCGGGACCCT CGAATCACAG CTTGAAGCTA CCAAACGCAA ACACCAGGAG 
ATTCGAGCTA TGAGAAGTCA GCTCAAGAAG ATTGAAGACC TGGGGGCGGC CATGGAGGAA 
GCGCTGATCC TGGACAACAA GTACACGGAG CACAGCACCG TGGGCCTGGC CCAGCAGTGG 
GACCAGCTCG ACCAGTTGGG CATGCGCATG CAGCACAACC TGGAGCAGCA GATCCAGGCC 
AGGAACACGA CCGGAGTGAC CGAGGAGGCC CTCAAAGAAT TCAGCATGAT GTTCAAACAC 
TTCGACAAGG ACAAGTCTGG CCGGCTGAAC CACCAGGAGT TCAAATCCTG TCTGCGCTCC 
CTGGGCTATG ACCTGCCCAT GGTGGAGGAA GGGGAGCCCG ACCCTGAGTT CGAGGCCATC 
CTGGACACTG TGGATCCTAA CAGGGACGGC CACGTCTCCC TGCAAGAGTA CATGGCCTTT 
ATGATCAGCC GTGAGACCGA GAACGTCAAG TCCAGCGAGG AGATTGAGAG CGCCTTCCGG 
GCCCTGAGCT CTGAAGGGAA GCCTTATGTG ACCAAGGAGG AGCTGTACCA GAACCTGACT 
CGGGAACAAG CCGACTACTG CGTCTCCCAC ATGAAGCCCT ACGTGGACGG CAAGGGCCGC 
GAACTCCCCA CTGCTTTTGA CTACGTCGAG TTCACCCGCT CGCTCTTCGT GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854800.1
CDS	121..7557
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLHQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMYRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVDHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWESLKSKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGAMPRDESDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKEKLDVLDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQSFADQLIAGGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDMGNSLIDRGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDAINGRFQKIKSMAASRRARLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLVKKNNHHEENISSKMKGLNGKVSDLEKAASQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHAALMKRWSQLLANSATRKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGIAYRRVIRVYQYEVGDDLSGRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_024999032.1
Organism	Bos taurus(cattle)
Definition	Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999032.1

ORF Insert Sequence:

ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT 
GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT 
CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC 
AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG 
AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC 
CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG 
AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT 
GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC 
CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC 
CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC 
CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT 
AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT 
GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG 
GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC 
ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC 
TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG 
AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG 
GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA 
GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG 
AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG 
CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG 
GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA 
ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG 
AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT 
GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT 
TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG 
CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT 
GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG 
AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG 
CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT 
CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC 
GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA 
GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG 
GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT 
CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG 
AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC 
AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC 
GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC 
CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC 
GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA 
GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC 
GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC 
AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC 
GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC 
AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGTACC ATTCTCTGCT GGAACTGGGA 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG 
AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC 
GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC 
AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA 
GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC 
AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG 
CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA 
GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC 
TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG 
GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC 
CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA 
TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC 
CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT 
CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG 
TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC 
GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG 
ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG 
GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG 
ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC 
GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG 
ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA 
GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG 
GACCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ATCAGAAGCA CCAGGCCTTC 
GAGGCAGAAC TGCACGCCAA TGCGGACCGG ATCCGTGGGG TCATCGACAT GGGCAACTCC 
CTCATTGACC GCGGGGCCTG CGCCGGCAGC GAGGATGCCG TCAAGGCCCG GCTGGCCGCC 
TTAGCTGACC AGTGGCAGTT CCTGGTGCAG AAGTCGGCTG AGAAGAGCCA GAAGCTGAAA 
GAAGCCAACA AGCAGCAGAA CTTCAACACT GGCATCAAGG ACTTCGACTT CTGGCTCTCT 
GAGGTGGAAG CCCTGCTGGC CTCTGAGGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC 
TTGCTAAAAA AACATCAGCT GCTGGAGGCA GATATCTCTG CCCACGAGGA CCGCCTGAAG 
GACCTCAACA GCCAGGCGGA CAGCCTGATG ACCAGCAGCG CCTTCGACAC CTCCCAGGTG 
AAGGACAAGC GGGACGCCAT CAACGGCCGT TTCCAGAAGA TCAAGAGCAT GGCCGCCTCG 
AGGCGAGCTA GGCTGAATGA GTCCCACCGC CTGCACCAGT TTTTCCGGGA CATGGATGAT 
GAGGAGTCCT GGATCAAGGA AAAGAAGCTG CTGGTGAGCT CAGAGGACTA CGGCCGTGAC 
CTGACGGGCG TCCAGAACCT GAGGAAGAAG CACAAACGGC TGGAAGCAGA GCTGGCTGCC 
CACGAGCCGG CCATTCAGGG TGTCCTGGAC ACTGGAAAGA AGCTGTCTGA TGACAACACC 
ATCGGGAAGG AGGAGATTCA GCAGCGCCTG GCGCAGTTCG TGGAGCACTG GAAGGAGCTG 
AAGCAGCTGG CAGCTGCCCG GGGCCAGCGG CTAGAAGAAT CTTTGGAATA TCAGCAGTTT 
GTAGCCAACG TGGAGGAGGA GGAAGCCTGG ATCAATGAGA AGATGACCCT GGTGGCCAGT 
GAAGATTATG GAGACACTCT TGCTGCCATC CAGGGCTTGC TGAAGAAACA CGAAGCCTTC 
GAGACGGACT TCACTGTCCA CAAGGACCGA GTGAATGATG TCTGCACCAA CGGCCAGGAC 
CTGGTTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CCAAGATGAA GGGCTTGAAC 
GGGAAAGTGT CAGACTTGGA GAAGGCTGCC TCCCAGAGGA AGGCCAAGCT CGACGAGAAC 
TCGGCCTTCC TCCAGTTCAA CTGGAAAGCC GATGTGGTGG AGTCCTGGAT CGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGT CGAGATCTGT CTTCTGTCCA AACTCTCCTC 
ACCAAACAGG AAACCTTCGA TGCTGGCCTG CAGGCCTTCC AGCAGGAGGG CATCGCCAAC 
ATCACTGCCC TCAAAGATCA GCTGCTGGCC GCCAAGCACG TTCAGTCCAA GGCCATCGAG 
GCCCGCCATG CTGCCCTCAT GAAGAGGTGG AGCCAGCTCC TGGCCAACTC GGCCACCCGG 
AAGAAGAAGC TGCTGGAGGC ACAGAGTCAC TTCCGCAAGG TGGAGGATCT CTTCCTGACC 
TTTGCTAAAA AGGCTTCAGC CTTCAACAGC TGGTTTGAAA ACGCAGAGGA GGACTTAACC 
GACCCAGTGC GCTGCAACTC TCTGGAAGAG ATCAAGGCCT TGAGAGAGGC CCACGACGCC 
TTCCGCTCCT CGTTGAGCTC GGCCCAGGCC GACTTCAACC AGCTGGCAGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT GGCCTCCAAC CCCTACACCT GGTTCACCAT GGAGGCCCTG 
GAGGAGACCT GGAGAAACCT ACAGAAGATC ATCAAGGAAA GGGAGCTGGA GCTGCAGAAG 
GAGCAGCGAC GGCAGGAGGA GAATGACAAG CTGCGCCAGG AGTTCGCCCA GCATGCCAAC 
GCCTTCCACC AGTGGATCCA GGAGACCAGA ACGTACCTCC TCGATGGCAT AGCGTATCGT 
CGGGTCATTC GTGTCTATCA GTATGAAGTT GGGGATGATC TGTCTGGAAG GTCCTGCATG 
GTGGAAGAAT CCGGGACCCT CGAATCACAG CTTGAAGCTA CCAAACGCAA ACACCAGGAG 
ATTCGAGCTA TGAGAAGTCA GCTCAAGAAG ATTGAAGACC TGGGGGCGGC CATGGAGGAA 
GCGCTGATCC TGGACAACAA GTACACGGAG CACAGCACCG TGGGCCTGGC CCAGCAGTGG 
GACCAGCTCG ACCAGTTGGG CATGCGCATG CAGCACAACC TGGAGCAGCA GATCCAGGCC 
AGGAACACGA CCGGAGTGAC CGAGGAGGCC CTCAAAGAAT TCAGCATGAT GTTCAAACAC 
TTCGACAAGG ACAAGTCTGG CCGGCTGAAC CACCAGGAGT TCAAATCCTG TCTGCGCTCC 
CTGGGCTATG ACCTGCCCAT GGTGGAGGAA GGGGAGCCCG ACCCTGAGTT CGAGGCCATC 
CTGGACACTG TGGATCCTAA CAGGGACGGC CACGTCTCCC TGCAAGAGTA CATGGCCTTT 
ATGATCAGCC GTGAGACCGA GAACGTCAAG TCCAGCGAGG AGATTGAGAG CGCCTTCCGG 
GCCCTGAGCT CTGAAGGGAA GCCTTATGTG ACCAAGGAGG AGCTGTACCA GAACCTGACT 
CGGGAACAAG CCGACTACTG CGTCTCCCAC ATGAAGCCCT ACGTGGACGG CAAGGGCCGC 
GAACTCCCCA CTGCTTTTGA CTACGTCGAG TTCACCCGCT CGCTCTTCGT GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_024999036.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854804.1 Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OBa54552
Clone ID Related Accession (Same CDS sequence)	XM_024999036.1
Accession Version	XM_024999036.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7416bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT 
GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT 
CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC 
AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG 
AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC 
CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG 
AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT 
GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC 
CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC 
CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC 
CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT 
AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT 
GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG 
GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC 
ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC 
TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG 
AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG 
GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA 
GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG 
AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG 
CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG 
GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA 
ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG 
AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT 
GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT 
TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG 
CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT 
GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG 
AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG 
CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT 
CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC 
GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA 
GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG 
GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT 
CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG 
AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC 
AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC 
GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC 
CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC 
GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA 
GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC 
GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC 
AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC 
GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC 
AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGACGC GCGTAACTAA GGAGGCTGGC 
AGCGTGTCTC TGCGTATGAA ACAGGTGGAA GAACTGTACC ATTCTCTGCT GGAACTGGGA 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG 
AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC 
GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC 
AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA 
GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC 
AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG 
CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA 
GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC 
TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG 
GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC 
CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA 
TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC 
CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT 
CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG 
TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC 
GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG 
ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG 
GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG 
ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC 
GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG 
ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA 
GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG 
GACCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ATCAGAAGCA CCAGGCCTTC 
GAGGCAGAAC TGCACGCCAA TGCGGACCGG ATCCGTGGGG TCATCGACAT GGGCAACTCC 
CTCATTGACC GCGGGGCCTG CGCCGGCAGC GAGGATGCCG TCAAGGCCCG GCTGGCCGCC 
TTAGCTGACC AGTGGCAGTT CCTGGTGCAG AAGTCGGCTG AGAAGAGCCA GAAGCTGAAA 
GAAGCCAACA AGCAGCAGAA CTTCAACACT GGCATCAAGG ACTTCGACTT CTGGCTCTCT 
GAGGTGGAAG CCCTGCTGGC CTCTGAGGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC 
TTGCTAAAAA AACATCAGCT GCTGGAGGCA GATATCTCTG CCCACGAGGA CCGCCTGAAG 
GACCTCAACA GCCAGGCGGA CAGCCTGATG ACCAGCAGCG CCTTCGACAC CTCCCAGGTG 
AAGGACAAGC GGGACGCCAT CAACGGCCGT TTCCAGAAGA TCAAGAGCAT GGCCGCCTCG 
AGGCGAGCTA GGCTGAATGA GTCCCACCGC CTGCACCAGT TTTTCCGGGA CATGGATGAT 
GAGGAGTCCT GGATCAAGGA AAAGAAGCTG CTGGTGAGCT CAGAGGACTA CGGCCGTGAC 
CTGACGGGCG TCCAGAACCT GAGGAAGAAG CACAAACGGC TGGAAGCAGA GCTGGCTGCC 
CACGAGCCGG CCATTCAGGG TGTCCTGGAC ACTGGAAAGA AGCTGTCTGA TGACAACACC 
ATCGGGAAGG AGGAGATTCA GCAGCGCCTG GCGCAGTTCG TGGAGCACTG GAAGGAGCTG 
AAGCAGCTGG CAGCTGCCCG GGGCCAGCGG CTAGAAGAAT CTTTGGAATA TCAGCAGTTT 
GTAGCCAACG TGGAGGAGGA GGAAGCCTGG ATCAATGAGA AGATGACCCT GGTGGCCAGT 
GAAGATTATG GAGACACTCT TGCTGCCATC CAGGGCTTGC TGAAGAAACA CGAAGCCTTC 
GAGACGGACT TCACTGTCCA CAAGGACCGA GTGAATGATG TCTGCACCAA CGGCCAGGAC 
CTGGTTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CCAAGATGAA GGGCTTGAAC 
GGGAAAGTGT CAGACTTGGA GAAGGCTGCC TCCCAGAGGA AGGCCAAGCT CGACGAGAAC 
TCGGCCTTCC TCCAGTTCAA CTGGAAAGCC GATGTGGTGG AGTCCTGGAT CGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGT CGAGATCTGT CTTCTGTCCA AACTCTCCTC 
ACCAAACAGG AAACCTTCGA TGCTGGCCTG CAGGCCTTCC AGCAGGAGGG CATCGCCAAC 
ATCACTGCCC TCAAAGATCA GCTGCTGGCC GCCAAGCACG TTCAGTCCAA GGCCATCGAG 
GCCCGCCATG CTGCCCTCAT GAAGAGGTGG AGCCAGCTCC TGGCCAACTC GGCCACCCGG 
AAGAAGAAGC TGCTGGAGGC ACAGAGTCAC TTCCGCAAGG TGGAGGATCT CTTCCTGACC 
TTTGCTAAAA AGGCTTCAGC CTTCAACAGC TGGTTTGAAA ACGCAGAGGA GGACTTAACC 
GACCCAGTGC GCTGCAACTC TCTGGAAGAG ATCAAGGCCT TGAGAGAGGC CCACGACGCC 
TTCCGCTCCT CGTTGAGCTC GGCCCAGGCC GACTTCAACC AGCTGGCAGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT GGCCTCCAAC CCCTACACCT GGTTCACCAT GGAGGCCCTG 
GAGGAGACCT GGAGAAACCT ACAGAAGATC ATCAAGGAAA GGGAGCTGGA GCTGCAGAAG 
GAGCAGCGAC GGCAGGAGGA GAATGACAAG CTGCGCCAGG AGTTCGCCCA GCATGCCAAC 
GCCTTCCACC AGTGGATCCA GGAGACCAGG TCCTGCATGG TGGAAGAATC CGGGACCCTC 
GAATCACAGC TTGAAGCTAC CAAACGCAAA CACCAGGAGA TTCGAGCTAT GAGAAGTCAG 
CTCAAGAAGA TTGAAGACCT GGGGGCGGCC ATGGAGGAAG CGCTGATCCT GGACAACAAG 
TACACGGAGC ACAGCACCGT GGGCCTGGCC CAGCAGTGGG ACCAGCTCGA CCAGTTGGGC 
ATGCGCATGC AGCACAACCT GGAGCAGCAG ATCCAGGCCA GGAACACGAC CGGAGTGACC 
GAGGAGGCCC TCAAAGAATT CAGCATGATG TTCAAACACT TCGACAAGGA CAAGTCTGGC 
CGGCTGAACC ACCAGGAGTT CAAATCCTGT CTGCGCTCCC TGGGCTATGA CCTGCCCATG 
GTGGAGGAAG GGGAGCCCGA CCCTGAGTTC GAGGCCATCC TGGACACTGT GGATCCTAAC 
AGGGACGGCC ACGTCTCCCT GCAAGAGTAC ATGGCCTTTA TGATCAGCCG TGAGACCGAG 
AACGTCAAGT CCAGCGAGGA GATTGAGAGC GCCTTCCGGG CCCTGAGCTC TGAAGGGAAG 
CCTTATGTGA CCAAGGAGGA GCTGTACCAG AACCTGACTC GGGAACAAGC CGACTACTGC 
GTCTCCCACA TGAAGCCCTA CGTGGACGGC AAGGGCCGCG AACTCCCCAC TGCTTTTGAC 
TACGTCGAGT TCACCCGCTC GCTCTTCGTG AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854804.1
CDS	136..7551
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLHQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMYRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVDHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWESLKSKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRVTKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGAMPRDESDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKEKLDVLDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQSFADQLIAGGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDMGNSLIDRGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDAINGRFQKIKSMAASRRARLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLVKKNNHHEENISSKMKGLNGKVSDLEKAASQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHAALMKRWSQLLANSATRKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_024999036.1
Organism	Bos taurus(cattle)
Definition	Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999036.1

ORF Insert Sequence:

ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT 
GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT 
CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC 
AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG 
AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC 
CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG 
AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT 
GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC 
CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC 
CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC 
CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT 
AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT 
GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG 
GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC 
ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC 
TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG 
AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG 
GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA 
GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG 
AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG 
CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG 
GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA 
ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG 
AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT 
GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT 
TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG 
CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT 
GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG 
AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG 
CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT 
CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC 
GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA 
GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG 
GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT 
CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG 
AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC 
AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC 
GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC 
CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC 
GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA 
GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC 
GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC 
AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC 
GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC 
AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGACGC GCGTAACTAA GGAGGCTGGC 
AGCGTGTCTC TGCGTATGAA ACAGGTGGAA GAACTGTACC ATTCTCTGCT GGAACTGGGA 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG 
AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC 
GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC 
AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA 
GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC 
AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG 
CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA 
GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC 
TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG 
GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC 
CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA 
TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC 
CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT 
CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG 
TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC 
GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG 
ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG 
GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG 
ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC 
GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG 
ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA 
GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG 
GACCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ATCAGAAGCA CCAGGCCTTC 
GAGGCAGAAC TGCACGCCAA TGCGGACCGG ATCCGTGGGG TCATCGACAT GGGCAACTCC 
CTCATTGACC GCGGGGCCTG CGCCGGCAGC GAGGATGCCG TCAAGGCCCG GCTGGCCGCC 
TTAGCTGACC AGTGGCAGTT CCTGGTGCAG AAGTCGGCTG AGAAGAGCCA GAAGCTGAAA 
GAAGCCAACA AGCAGCAGAA CTTCAACACT GGCATCAAGG ACTTCGACTT CTGGCTCTCT 
GAGGTGGAAG CCCTGCTGGC CTCTGAGGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC 
TTGCTAAAAA AACATCAGCT GCTGGAGGCA GATATCTCTG CCCACGAGGA CCGCCTGAAG 
GACCTCAACA GCCAGGCGGA CAGCCTGATG ACCAGCAGCG CCTTCGACAC CTCCCAGGTG 
AAGGACAAGC GGGACGCCAT CAACGGCCGT TTCCAGAAGA TCAAGAGCAT GGCCGCCTCG 
AGGCGAGCTA GGCTGAATGA GTCCCACCGC CTGCACCAGT TTTTCCGGGA CATGGATGAT 
GAGGAGTCCT GGATCAAGGA AAAGAAGCTG CTGGTGAGCT CAGAGGACTA CGGCCGTGAC 
CTGACGGGCG TCCAGAACCT GAGGAAGAAG CACAAACGGC TGGAAGCAGA GCTGGCTGCC 
CACGAGCCGG CCATTCAGGG TGTCCTGGAC ACTGGAAAGA AGCTGTCTGA TGACAACACC 
ATCGGGAAGG AGGAGATTCA GCAGCGCCTG GCGCAGTTCG TGGAGCACTG GAAGGAGCTG 
AAGCAGCTGG CAGCTGCCCG GGGCCAGCGG CTAGAAGAAT CTTTGGAATA TCAGCAGTTT 
GTAGCCAACG TGGAGGAGGA GGAAGCCTGG ATCAATGAGA AGATGACCCT GGTGGCCAGT 
GAAGATTATG GAGACACTCT TGCTGCCATC CAGGGCTTGC TGAAGAAACA CGAAGCCTTC 
GAGACGGACT TCACTGTCCA CAAGGACCGA GTGAATGATG TCTGCACCAA CGGCCAGGAC 
CTGGTTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CCAAGATGAA GGGCTTGAAC 
GGGAAAGTGT CAGACTTGGA GAAGGCTGCC TCCCAGAGGA AGGCCAAGCT CGACGAGAAC 
TCGGCCTTCC TCCAGTTCAA CTGGAAAGCC GATGTGGTGG AGTCCTGGAT CGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGT CGAGATCTGT CTTCTGTCCA AACTCTCCTC 
ACCAAACAGG AAACCTTCGA TGCTGGCCTG CAGGCCTTCC AGCAGGAGGG CATCGCCAAC 
ATCACTGCCC TCAAAGATCA GCTGCTGGCC GCCAAGCACG TTCAGTCCAA GGCCATCGAG 
GCCCGCCATG CTGCCCTCAT GAAGAGGTGG AGCCAGCTCC TGGCCAACTC GGCCACCCGG 
AAGAAGAAGC TGCTGGAGGC ACAGAGTCAC TTCCGCAAGG TGGAGGATCT CTTCCTGACC 
TTTGCTAAAA AGGCTTCAGC CTTCAACAGC TGGTTTGAAA ACGCAGAGGA GGACTTAACC 
GACCCAGTGC GCTGCAACTC TCTGGAAGAG ATCAAGGCCT TGAGAGAGGC CCACGACGCC 
TTCCGCTCCT CGTTGAGCTC GGCCCAGGCC GACTTCAACC AGCTGGCAGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT GGCCTCCAAC CCCTACACCT GGTTCACCAT GGAGGCCCTG 
GAGGAGACCT GGAGAAACCT ACAGAAGATC ATCAAGGAAA GGGAGCTGGA GCTGCAGAAG 
GAGCAGCGAC GGCAGGAGGA GAATGACAAG CTGCGCCAGG AGTTCGCCCA GCATGCCAAC 
GCCTTCCACC AGTGGATCCA GGAGACCAGG TCCTGCATGG TGGAAGAATC CGGGACCCTC 
GAATCACAGC TTGAAGCTAC CAAACGCAAA CACCAGGAGA TTCGAGCTAT GAGAAGTCAG 
CTCAAGAAGA TTGAAGACCT GGGGGCGGCC ATGGAGGAAG CGCTGATCCT GGACAACAAG 
TACACGGAGC ACAGCACCGT GGGCCTGGCC CAGCAGTGGG ACCAGCTCGA CCAGTTGGGC 
ATGCGCATGC AGCACAACCT GGAGCAGCAG ATCCAGGCCA GGAACACGAC CGGAGTGACC 
GAGGAGGCCC TCAAAGAATT CAGCATGATG TTCAAACACT TCGACAAGGA CAAGTCTGGC 
CGGCTGAACC ACCAGGAGTT CAAATCCTGT CTGCGCTCCC TGGGCTATGA CCTGCCCATG 
GTGGAGGAAG GGGAGCCCGA CCCTGAGTTC GAGGCCATCC TGGACACTGT GGATCCTAAC 
AGGGACGGCC ACGTCTCCCT GCAAGAGTAC ATGGCCTTTA TGATCAGCCG TGAGACCGAG 
AACGTCAAGT CCAGCGAGGA GATTGAGAGC GCCTTCCGGG CCCTGAGCTC TGAAGGGAAG 
CCTTATGTGA CCAAGGAGGA GCTGTACCAG AACCTGACTC GGGAACAAGC CGACTACTGC 
GTCTCCCACA TGAAGCCCTA CGTGGACGGC AAGGGCCGCG AACTCCCCAC TGCTTTTGAC 
TACGTCGAGT TCACCCGCTC GCTCTTCGTG AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_015473653.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329139.1 Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa92000
Clone ID Related Accession (Same CDS sequence)	XM_015473653.1
Accession Version	XM_015473653.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7533bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGCGG GGTCAAAGTG 
CTGGAGACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTGGACAG ATACCACCGC 
TTCAAGGAGC TCTCGACCCT GAGGCGCCAG AAGCTTGAAG ACTCCTACCG ATTCCAGTTT 
TTCCAAAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAGCTTCA GATTGCCTCA 
GATGAGAATT ACAAAGACCC AACCAACCTG CAGGGCAAGC TGCAGAAGCA TCAAGCCTTC 
GAAGCCGAGG TGCAGGCCAA CTCGGGCGCC ATCGTGAAGC TGGATGAGAC TGGTAACCTG 
ATGATCTCAG AAGGGCACTT TGCATCCGAA ACCATCCGGA CTCGTCTGAT GGAGCTGCAC 
CGCCAGTGGG AATTGCTCCT GGAGAAGATG CGGGAGAAAG GCATCAAGCT GCTGCAGGCC 
CAGAAGCTGG TGCAGTACCT GCGGGAGTGT GAGGATGTGA TGGACTGGAT CAATGACAAG 
GAAGCCATTG TGACGTCTGA GGAGCTGGGC CAGGACCTGG AGCACGTGGA GGTGTTACAG 
AAGAAATTCG AAGAGTTTCA GACAGACATG GCTGCCCACG AAGAGAGAGT CAATGAAGTG 
AACCAGTTCG CTGCCAAGCT CATTCAGGAG CAGCACCCTG AGGAGGAGCT GATCAAGACA 
AAGCAGGACG AGGTGAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCCCT CCAGCGGCAG 
GGGAAGCTGT TCGGGGCAGC TGAGGTCCAA CGCTTTAACA GGGATGTGGA CGAGACCATC 
AGCTGGATTA AGGAGAAGGA ACAGTTAATG GCCTCTGATG ACTTTGGCCG GGACCTGGCC 
AGCGTCCAGG CTTTACTACG GAAACACGAG GGTTTGGAGA GAGATCTTGC TGCCTTGGAG 
GACAAGGTCA AAGCCCTGTG TGCCGAGGCT GACCGCCTGC ATCAGTCCCA CCCTCTGAGT 
GCCACCCAGA TCCAGGTGAA GCGAGAGGAG CTGATCACCA ACTGGGAGCA GATCCGCACC 
CTGGCCGCGG AGAGACACGC ACGTCTCAAC GATTCCTACA GGCTTCAGCG CTTCCTTGCC 
GACTTCCGAG ACCTCACCAG CTGGGTCACT GAGATGAAAG CCCTCATCAA CGCAGATGAA 
CTTGCCAACG ATGTGGCCGG AGCCGAAGCC CTGCTGGATA GGCATCAGGA GCACAAGGGT 
GAAATTGACG CCCATGAAGA TAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT 
GCAGCTGGTC ACTACGCCTC AGATGAAGTG AGGGAGAAGC TTACCGTGCT GTCCGAGGAG 
CGGGCCGCGC TGCTGGAGCT CTGGGAGCTG CGACGGCAGC AATATGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ACGAAGACCT GGGCGATTCC TTGGATAGTG TGGAAGCGCT TCTAAAGAAG 
CACGAAGACT TTGAGAAGTC CCTCAGCGCC CAGGAGGAGA AGATCACAGC CTTAGATGAG 
TTTGCCACCA AGCTGATTCA GAACAACCAC TACGCAATGG AAGATGTGGC CACCCGGCGA 
GACGCACTGC TGAGCCGTCG CAATGCCCTG CATGAGAGAG CCATGTACCG CCGTGCCCAG 
CTTGCAGATT CCTTCCACTT GCAGCAGTTT TTCCGTGACT CTGATGAGCT CAAGAGTTGG 
GTCAACGAGA AGATGAAAAC AGCCACGGAT GAAGCTTATA AAGACCCATC CAACCTACAA 
GGGAAAGTGC AGAAGCACCA GGCCTTTGAG GCCGAGCTCT CGGCAAACCA GAGCCGCATC 
GACGCCCTGG AGAAAGCCGG GCAGAAGCTG ATCGATGTCG ACCACTATGC CAAGGACGAG 
GTGGCGGCTC GGATGAATGA GGTCATCAGT CTGTGGAAGA AGCTGCTGGA GGCCACAGAG 
CTGAAAGGCA TAAAGCTCCG CGAGGCTAAC CAGCAGCAGC AGTTTAATCG CAACGTTGAG 
GACATCGAGC TGTGGCTGTA TGAAGTGGAA GGTCATCTGG CCTCGGACGA CTACGGCAAA 
GACCTCACCA ACGTCCAGAA CCTCCAGAAG AAGCACGCCC TGCTGGAGGC TGACGTGGCA 
GCTCACCAGG ATCGCATCGA TGGCATCACT ATCCAAGCCC GCCAGTTCCA GGACGCGGGC 
CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAGGCCC TGGTAGCTCG CTACGAGGCG 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCTG ATTCCCTGCG GTTGCAGCAG 
CTCTTCCGTG ACGTCGAGGA TGAGGAGACG TGGATTCGGG AGAAAGAACC CATTGCCGCC 
TCCACCAACA GAGGCAAAGA TTTAATTGGA GTCCAGAACC TGCTAAAGAA ACACCAAGCG 
CTACAAGCAG AAATTGCTGG CCACGAGCCC CGCATCAAAG CAGTCACACA GAAGGGGAAC 
GCCATGGTGG AGGAGGGCCA TTTTGCTGCT GAGGACGTGA AGGCCAAGCT CCATGAGCTG 
AACCAGAAGT GGGAGTCGCT GAAGTCCAAG GCCTCCCAGC GGCGGCAGGA CCTGGAGGAC 
TCGCTGCAGG CCCAGCAGTA CTTTGCCGAC GCCAACGAGG CTGAGTCCTG GATGCGGGAG 
AAGGAGCCCA TCGTGGGCAG CACCGACTAT GGCAAAGATG AGGACTCGGC CGAGGCTCTG 
CTAAAGAAGC ACGAGGCATT GATGTCGGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCC 
CTGCGAGAGC AGGCCCAGTC GTGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG 
AAGGAGCTGG TCTTGGCGCT CTATGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAAGGAG ACATTCTCAC CTTACTCAAC AGCACCAACA AGGACTGGTG GAAAGTGGAA 
GTGAATGACC GGCAGGGCTT CGTGCCGGCT GCCTACGTGA AGAAGCTGGA TCCTGCGCAG 
TCGGCCTCCC GGGAGAACCT CCTGGAAGAG CAGGGCAGCA TCGCGCTGCG GCAGGAACAG 
ATCGACAACC AGACGCGCGT AACTAAGGAG GCTGGCAGCG TGTCTCTGCG TATGAAACAG 
GTGGAAGAAC TGTACCATTC TCTGCTGGAA CTGGGAGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGCTGTA AGAAGTTCAT GTTGTTCCGT GAAGCGAACG AGCTACAGCA GTGGATCAAT 
GAGAAAGAAG CGGCCCTGAC AAGTGAGGAG GTTGGCGCGG ACTTGGAGCA GGTTGAAGTG 
CTGCAGAAGA AGTTTGACGA CTTCCAGAAG GATCTCAAGG CCAATGAGTC GCGGCTGAAG 
GACATTAACA AGGTGGCTGA AGACCTGGAG TCGGAAGGTC TGATGGCGGA GGAGGTGCAG 
GCCGTGCAGC AGCAGGAGGT GTATGGCGCG ATGCCCAGGG ATGAATCTGA TTCCAAGACA 
GCCTCCCCTT GGAAGTCTGC TCGCCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTGA ACGAGCGCTG GCGGTCCCTG CAGCAGCTGG CTGAGGAGCG GAGCCAACTC 
TTGGGCAGTG CCCATGAAGT GCAGCGGTTC CACAGAGATG CTGACGAAAC CAAAGAGTGG 
ATCGAAGAGA AGAACCAGGC TCTAAACACA GACAACTACG GCCACGACCT GGCCAGTGTC 
CAGGCCCTGC AGCGCAAGCA CGAGGGCTTC GAGAGGGACC TCGCAGCCCT TGGTGACAAG 
GTGAACTCGC TCGGGGAGAC AGCCGAGCGC CTGATCCAGT CCCACCCCGA GTCGGCAGAG 
GACCTGCAGG AGAAGTGCAC TGAGCTGAAC CAGGCATGGA GCAGCCTGGG CAAGCGTGCC 
GACCAGCGCA AGGCCAAGCT GGGCGACTCC CACGACCTGC AGCGCTTCCT CAGCGACTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATC CGAGGTCTGG TGTCCTCAGA CGAGCTAGCC 
AAGGATGTCA CCGGAGCTGA GGCCCTGCTG GAGCGACACC AGGAACACCG GACAGAAATT 
GATGCCAGAG CTGGCACTTT CCAGGCATTC GAGCAGTTTG GGCAGCAGCT GTTGGCTCAT 
GGGCACTACG CCAGCCCCGA GATCAAGGAG AAGCTCGATG TTCTAGACCA GGAGCGTGCA 
GACCTTGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAATTGCAG 
CTGTTCCATC GGGATTGCGA GCAGGCCGAG AACTGGATGG CTGCCCGAGA GGCCTTTCTG 
AACACAGAAG ACAAAGGGGA CTCGCTAGAC AGTGTGGAGG CTCTGATCAA AAAACACGAG 
GACTTTGATA AAGCAATTAA TGTCCAGGAG GAGAAGATTG CTGCCCTGCA GTCCTTTGCT 
GACCAGCTCA TCGCTGGTGG CCACTACGCC AAAGGCGACA TCTCCAGCCG GCGCAATGAG 
GTTCTGGACA GGTGGCGCCG TCTAAAAGCT CAGATGATTG AGAAGAGGTC AAAGCTGGGA 
GAATCACAGA CCCTCCAGCA GTTCAGCCGG GATGTAGACG AGATTGAAGC TTGGATCAGT 
GAAAAATTAC AGACTGCAAG TGATGAGTCA TATAAGGACC CCACCAACAT CCAGCTTTCC 
AAGCTGCTGA GCAAGCATCA GAAGCACCAG GCCTTCGAGG CAGAACTGCA CGCCAATGCG 
GACCGGATCC GTGGGGTCAT CGACATGGGC AACTCCCTCA TTGACCGCGG GGCCTGCGCC 
GGCAGCGAGG ATGCCGTCAA GGCCCGGCTG GCCGCCTTAG CTGACCAGTG GCAGTTCCTG 
GTGCAGAAGT CGGCTGAGAA GAGCCAGAAG CTGAAAGAAG CCAACAAGCA GCAGAACTTC 
AACACTGGCA TCAAGGACTT CGACTTCTGG CTCTCTGAGG TGGAAGCCCT GCTGGCCTCT 
GAGGATTATG GCAAAGACCT GGCTTCTGTG AACAACTTGC TAAAAAAACA TCAGCTGCTG 
GAGGCAGATA TCTCTGCCCA CGAGGACCGC CTGAAGGACC TCAACAGCCA GGCGGACAGC 
CTGATGACCA GCAGCGCCTT CGACACCTCC CAGGTGAAGG ACAAGCGGGA CGCCATCAAC 
GGCCGTTTCC AGAAGATCAA GAGCATGGCC GCCTCGAGGC GAGCTAGGCT GAATGAGTCC 
CACCGCCTGC ACCAGTTTTT CCGGGACATG GATGATGAGG AGTCCTGGAT CAAGGAAAAG 
AAGCTGCTGG TGAGCTCAGA GGACTACGGC CGTGACCTGA CGGGCGTCCA GAACCTGAGG 
AAGAAGCACA AACGGCTGGA AGCAGAGCTG GCTGCCCACG AGCCGGCCAT TCAGGGTGTC 
CTGGACACTG GAAAGAAGCT GTCTGATGAC AACACCATCG GGAAGGAGGA GATTCAGCAG 
CGCCTGGCGC AGTTCGTGGA GCACTGGAAG GAGCTGAAGC AGCTGGCAGC TGCCCGGGGC 
CAGCGGCTAG AAGAATCTTT GGAATATCAG CAGTTTGTAG CCAACGTGGA GGAGGAGGAA 
GCCTGGATCA ATGAGAAGAT GACCCTGGTG GCCAGTGAAG ATTATGGAGA CACTCTTGCT 
GCCATCCAGG GCTTGCTGAA GAAACACGAA GCCTTCGAGA CGGACTTCAC TGTCCACAAG 
GACCGAGTGA ATGATGTCTG CACCAACGGC CAGGACCTGG TTAAGAAGAA CAATCACCAT 
GAGGAGAACA TCTCTTCCAA GATGAAGGGC TTGAACGGGA AAGTGTCAGA CTTGGAGAAG 
GCTGCCTCCC AGAGGAAGGC CAAGCTCGAC GAGAACTCGG CCTTCCTCCA GTTCAACTGG 
AAAGCCGATG TGGTGGAGTC CTGGATCGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT 
TATGGTCGAG ATCTGTCTTC TGTCCAAACT CTCCTCACCA AACAGGAAAC CTTCGATGCT 
GGCCTGCAGG CCTTCCAGCA GGAGGGCATC GCCAACATCA CTGCCCTCAA AGATCAGCTG 
CTGGCCGCCA AGCACGTTCA GTCCAAGGCC ATCGAGGCCC GCCATGCTGC CCTCATGAAG 
AGGTGGAGCC AGCTCCTGGC CAACTCGGCC ACCCGGAAGA AGAAGCTGCT GGAGGCACAG 
AGTCACTTCC GCAAGGTGGA GGATCTCTTC CTGACCTTTG CTAAAAAGGC TTCAGCCTTC 
AACAGCTGGT TTGAAAACGC AGAGGAGGAC TTAACCGACC CAGTGCGCTG CAACTCTCTG 
GAAGAGATCA AGGCCTTGAG AGAGGCCCAC GACGCCTTCC GCTCCTCGTT GAGCTCGGCC 
CAGGCCGACT TCAACCAGCT GGCAGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTGGCC 
TCCAACCCCT ACACCTGGTT CACCATGGAG GCCCTGGAGG AGACCTGGAG AAACCTACAG 
AAGATCATCA AGGAAAGGGA GCTGGAGCTG CAGAAGGAGC AGCGACGGCA GGAGGAGAAT 
GACAAGCTGC GCCAGGAGTT CGCCCAGCAT GCCAACGCCT TCCACCAGTG GATCCAGGAG 
ACCAGAACGT ACCTCCTCGA TGGCATAGCG TATCGTCGGG TCATTCGTGT CTATCAGTAT 
GAAGTTGGGG ATGATCTGTC TGGAAGGTCC TGCATGGTGG AAGAATCCGG GACCCTCGAA 
TCACAGCTTG AAGCTACCAA ACGCAAACAC CAGGAGATTC GAGCTATGAG AAGTCAGCTC 
AAGAAGATTG AAGACCTGGG GGCGGCCATG GAGGAAGCGC TGATCCTGGA CAACAAGTAC 
ACGGAGCACA GCACCGTGGG CCTGGCCCAG CAGTGGGACC AGCTCGACCA GTTGGGCATG 
CGCATGCAGC ACAACCTGGA GCAGCAGATC CAGGCCAGGA ACACGACCGG AGTGACCGAG 
GAGGCCCTCA AAGAATTCAG CATGATGTTC AAACACTTCG ACAAGGACAA GTCTGGCCGG 
CTGAACCACC AGGAGTTCAA ATCCTGTCTG CGCTCCCTGG GCTATGACCT GCCCATGGTG 
GAGGAAGGGG AGCCCGACCC TGAGTTCGAG GCCATCCTGG ACACTGTGGA TCCTAACAGG 
GACGGCCACG TCTCCCTGCA AGAGTACATG GCCTTTATGA TCAGCCGTGA GACCGAGAAC 
GTCAAGTCCA GCGAGGAGAT TGAGAGCGCC TTCCGGGCCC TGAGCTCTGA AGGGAAGCCT 
TATGTGACCA AGGAGGAGCT GTACCAGAAC CTGACTCGGG AACAAGCCGA CTACTGCGTC 
TCCCACATGA AGCCCTACGT GGACGGCAAG GGCCGCGAAC TCCCCACTGC TTTTGACTAC 
GTCGAGTTCA CCCGCTCGCT CTTCGTGAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329139.1
CDS	55..7587
Translation	MLSSFRKRRVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLHQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMYRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVDHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWESLKSKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRVTKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGAMPRDESDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKEKLDVLDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQSFADQLIAGGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDMGNSLIDRGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDAINGRFQKIKSMAASRRARLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLVKKNNHHEENISSKMKGLNGKVSDLEKAASQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHAALMKRWSQLLANSATRKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGIAYRRVIRVYQYEVGDDLSGRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_015473653.1
Organism	Bos taurus(cattle)
Definition	Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473653.1

ORF Insert Sequence:

ATGCTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGCGG GGTCAAAGTG 
CTGGAGACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTGGACAG ATACCACCGC 
TTCAAGGAGC TCTCGACCCT GAGGCGCCAG AAGCTTGAAG ACTCCTACCG ATTCCAGTTT 
TTCCAAAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAGCTTCA GATTGCCTCA 
GATGAGAATT ACAAAGACCC AACCAACCTG CAGGGCAAGC TGCAGAAGCA TCAAGCCTTC 
GAAGCCGAGG TGCAGGCCAA CTCGGGCGCC ATCGTGAAGC TGGATGAGAC TGGTAACCTG 
ATGATCTCAG AAGGGCACTT TGCATCCGAA ACCATCCGGA CTCGTCTGAT GGAGCTGCAC 
CGCCAGTGGG AATTGCTCCT GGAGAAGATG CGGGAGAAAG GCATCAAGCT GCTGCAGGCC 
CAGAAGCTGG TGCAGTACCT GCGGGAGTGT GAGGATGTGA TGGACTGGAT CAATGACAAG 
GAAGCCATTG TGACGTCTGA GGAGCTGGGC CAGGACCTGG AGCACGTGGA GGTGTTACAG 
AAGAAATTCG AAGAGTTTCA GACAGACATG GCTGCCCACG AAGAGAGAGT CAATGAAGTG 
AACCAGTTCG CTGCCAAGCT CATTCAGGAG CAGCACCCTG AGGAGGAGCT GATCAAGACA 
AAGCAGGACG AGGTGAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCCCT CCAGCGGCAG 
GGGAAGCTGT TCGGGGCAGC TGAGGTCCAA CGCTTTAACA GGGATGTGGA CGAGACCATC 
AGCTGGATTA AGGAGAAGGA ACAGTTAATG GCCTCTGATG ACTTTGGCCG GGACCTGGCC 
AGCGTCCAGG CTTTACTACG GAAACACGAG GGTTTGGAGA GAGATCTTGC TGCCTTGGAG 
GACAAGGTCA AAGCCCTGTG TGCCGAGGCT GACCGCCTGC ATCAGTCCCA CCCTCTGAGT 
GCCACCCAGA TCCAGGTGAA GCGAGAGGAG CTGATCACCA ACTGGGAGCA GATCCGCACC 
CTGGCCGCGG AGAGACACGC ACGTCTCAAC GATTCCTACA GGCTTCAGCG CTTCCTTGCC 
GACTTCCGAG ACCTCACCAG CTGGGTCACT GAGATGAAAG CCCTCATCAA CGCAGATGAA 
CTTGCCAACG ATGTGGCCGG AGCCGAAGCC CTGCTGGATA GGCATCAGGA GCACAAGGGT 
GAAATTGACG CCCATGAAGA TAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT 
GCAGCTGGTC ACTACGCCTC AGATGAAGTG AGGGAGAAGC TTACCGTGCT GTCCGAGGAG 
CGGGCCGCGC TGCTGGAGCT CTGGGAGCTG CGACGGCAGC AATATGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ACGAAGACCT GGGCGATTCC TTGGATAGTG TGGAAGCGCT TCTAAAGAAG 
CACGAAGACT TTGAGAAGTC CCTCAGCGCC CAGGAGGAGA AGATCACAGC CTTAGATGAG 
TTTGCCACCA AGCTGATTCA GAACAACCAC TACGCAATGG AAGATGTGGC CACCCGGCGA 
GACGCACTGC TGAGCCGTCG CAATGCCCTG CATGAGAGAG CCATGTACCG CCGTGCCCAG 
CTTGCAGATT CCTTCCACTT GCAGCAGTTT TTCCGTGACT CTGATGAGCT CAAGAGTTGG 
GTCAACGAGA AGATGAAAAC AGCCACGGAT GAAGCTTATA AAGACCCATC CAACCTACAA 
GGGAAAGTGC AGAAGCACCA GGCCTTTGAG GCCGAGCTCT CGGCAAACCA GAGCCGCATC 
GACGCCCTGG AGAAAGCCGG GCAGAAGCTG ATCGATGTCG ACCACTATGC CAAGGACGAG 
GTGGCGGCTC GGATGAATGA GGTCATCAGT CTGTGGAAGA AGCTGCTGGA GGCCACAGAG 
CTGAAAGGCA TAAAGCTCCG CGAGGCTAAC CAGCAGCAGC AGTTTAATCG CAACGTTGAG 
GACATCGAGC TGTGGCTGTA TGAAGTGGAA GGTCATCTGG CCTCGGACGA CTACGGCAAA 
GACCTCACCA ACGTCCAGAA CCTCCAGAAG AAGCACGCCC TGCTGGAGGC TGACGTGGCA 
GCTCACCAGG ATCGCATCGA TGGCATCACT ATCCAAGCCC GCCAGTTCCA GGACGCGGGC 
CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAGGCCC TGGTAGCTCG CTACGAGGCG 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCTG ATTCCCTGCG GTTGCAGCAG 
CTCTTCCGTG ACGTCGAGGA TGAGGAGACG TGGATTCGGG AGAAAGAACC CATTGCCGCC 
TCCACCAACA GAGGCAAAGA TTTAATTGGA GTCCAGAACC TGCTAAAGAA ACACCAAGCG 
CTACAAGCAG AAATTGCTGG CCACGAGCCC CGCATCAAAG CAGTCACACA GAAGGGGAAC 
GCCATGGTGG AGGAGGGCCA TTTTGCTGCT GAGGACGTGA AGGCCAAGCT CCATGAGCTG 
AACCAGAAGT GGGAGTCGCT GAAGTCCAAG GCCTCCCAGC GGCGGCAGGA CCTGGAGGAC 
TCGCTGCAGG CCCAGCAGTA CTTTGCCGAC GCCAACGAGG CTGAGTCCTG GATGCGGGAG 
AAGGAGCCCA TCGTGGGCAG CACCGACTAT GGCAAAGATG AGGACTCGGC CGAGGCTCTG 
CTAAAGAAGC ACGAGGCATT GATGTCGGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCC 
CTGCGAGAGC AGGCCCAGTC GTGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG 
AAGGAGCTGG TCTTGGCGCT CTATGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAAGGAG ACATTCTCAC CTTACTCAAC AGCACCAACA AGGACTGGTG GAAAGTGGAA 
GTGAATGACC GGCAGGGCTT CGTGCCGGCT GCCTACGTGA AGAAGCTGGA TCCTGCGCAG 
TCGGCCTCCC GGGAGAACCT CCTGGAAGAG CAGGGCAGCA TCGCGCTGCG GCAGGAACAG 
ATCGACAACC AGACGCGCGT AACTAAGGAG GCTGGCAGCG TGTCTCTGCG TATGAAACAG 
GTGGAAGAAC TGTACCATTC TCTGCTGGAA CTGGGAGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGCTGTA AGAAGTTCAT GTTGTTCCGT GAAGCGAACG AGCTACAGCA GTGGATCAAT 
GAGAAAGAAG CGGCCCTGAC AAGTGAGGAG GTTGGCGCGG ACTTGGAGCA GGTTGAAGTG 
CTGCAGAAGA AGTTTGACGA CTTCCAGAAG GATCTCAAGG CCAATGAGTC GCGGCTGAAG 
GACATTAACA AGGTGGCTGA AGACCTGGAG TCGGAAGGTC TGATGGCGGA GGAGGTGCAG 
GCCGTGCAGC AGCAGGAGGT GTATGGCGCG ATGCCCAGGG ATGAATCTGA TTCCAAGACA 
GCCTCCCCTT GGAAGTCTGC TCGCCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTGA ACGAGCGCTG GCGGTCCCTG CAGCAGCTGG CTGAGGAGCG GAGCCAACTC 
TTGGGCAGTG CCCATGAAGT GCAGCGGTTC CACAGAGATG CTGACGAAAC CAAAGAGTGG 
ATCGAAGAGA AGAACCAGGC TCTAAACACA GACAACTACG GCCACGACCT GGCCAGTGTC 
CAGGCCCTGC AGCGCAAGCA CGAGGGCTTC GAGAGGGACC TCGCAGCCCT TGGTGACAAG 
GTGAACTCGC TCGGGGAGAC AGCCGAGCGC CTGATCCAGT CCCACCCCGA GTCGGCAGAG 
GACCTGCAGG AGAAGTGCAC TGAGCTGAAC CAGGCATGGA GCAGCCTGGG CAAGCGTGCC 
GACCAGCGCA AGGCCAAGCT GGGCGACTCC CACGACCTGC AGCGCTTCCT CAGCGACTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATC CGAGGTCTGG TGTCCTCAGA CGAGCTAGCC 
AAGGATGTCA CCGGAGCTGA GGCCCTGCTG GAGCGACACC AGGAACACCG GACAGAAATT 
GATGCCAGAG CTGGCACTTT CCAGGCATTC GAGCAGTTTG GGCAGCAGCT GTTGGCTCAT 
GGGCACTACG CCAGCCCCGA GATCAAGGAG AAGCTCGATG TTCTAGACCA GGAGCGTGCA 
GACCTTGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAATTGCAG 
CTGTTCCATC GGGATTGCGA GCAGGCCGAG AACTGGATGG CTGCCCGAGA GGCCTTTCTG 
AACACAGAAG ACAAAGGGGA CTCGCTAGAC AGTGTGGAGG CTCTGATCAA AAAACACGAG 
GACTTTGATA AAGCAATTAA TGTCCAGGAG GAGAAGATTG CTGCCCTGCA GTCCTTTGCT 
GACCAGCTCA TCGCTGGTGG CCACTACGCC AAAGGCGACA TCTCCAGCCG GCGCAATGAG 
GTTCTGGACA GGTGGCGCCG TCTAAAAGCT CAGATGATTG AGAAGAGGTC AAAGCTGGGA 
GAATCACAGA CCCTCCAGCA GTTCAGCCGG GATGTAGACG AGATTGAAGC TTGGATCAGT 
GAAAAATTAC AGACTGCAAG TGATGAGTCA TATAAGGACC CCACCAACAT CCAGCTTTCC 
AAGCTGCTGA GCAAGCATCA GAAGCACCAG GCCTTCGAGG CAGAACTGCA CGCCAATGCG 
GACCGGATCC GTGGGGTCAT CGACATGGGC AACTCCCTCA TTGACCGCGG GGCCTGCGCC 
GGCAGCGAGG ATGCCGTCAA GGCCCGGCTG GCCGCCTTAG CTGACCAGTG GCAGTTCCTG 
GTGCAGAAGT CGGCTGAGAA GAGCCAGAAG CTGAAAGAAG CCAACAAGCA GCAGAACTTC 
AACACTGGCA TCAAGGACTT CGACTTCTGG CTCTCTGAGG TGGAAGCCCT GCTGGCCTCT 
GAGGATTATG GCAAAGACCT GGCTTCTGTG AACAACTTGC TAAAAAAACA TCAGCTGCTG 
GAGGCAGATA TCTCTGCCCA CGAGGACCGC CTGAAGGACC TCAACAGCCA GGCGGACAGC 
CTGATGACCA GCAGCGCCTT CGACACCTCC CAGGTGAAGG ACAAGCGGGA CGCCATCAAC 
GGCCGTTTCC AGAAGATCAA GAGCATGGCC GCCTCGAGGC GAGCTAGGCT GAATGAGTCC 
CACCGCCTGC ACCAGTTTTT CCGGGACATG GATGATGAGG AGTCCTGGAT CAAGGAAAAG 
AAGCTGCTGG TGAGCTCAGA GGACTACGGC CGTGACCTGA CGGGCGTCCA GAACCTGAGG 
AAGAAGCACA AACGGCTGGA AGCAGAGCTG GCTGCCCACG AGCCGGCCAT TCAGGGTGTC 
CTGGACACTG GAAAGAAGCT GTCTGATGAC AACACCATCG GGAAGGAGGA GATTCAGCAG 
CGCCTGGCGC AGTTCGTGGA GCACTGGAAG GAGCTGAAGC AGCTGGCAGC TGCCCGGGGC 
CAGCGGCTAG AAGAATCTTT GGAATATCAG CAGTTTGTAG CCAACGTGGA GGAGGAGGAA 
GCCTGGATCA ATGAGAAGAT GACCCTGGTG GCCAGTGAAG ATTATGGAGA CACTCTTGCT 
GCCATCCAGG GCTTGCTGAA GAAACACGAA GCCTTCGAGA CGGACTTCAC TGTCCACAAG 
GACCGAGTGA ATGATGTCTG CACCAACGGC CAGGACCTGG TTAAGAAGAA CAATCACCAT 
GAGGAGAACA TCTCTTCCAA GATGAAGGGC TTGAACGGGA AAGTGTCAGA CTTGGAGAAG 
GCTGCCTCCC AGAGGAAGGC CAAGCTCGAC GAGAACTCGG CCTTCCTCCA GTTCAACTGG 
AAAGCCGATG TGGTGGAGTC CTGGATCGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT 
TATGGTCGAG ATCTGTCTTC TGTCCAAACT CTCCTCACCA AACAGGAAAC CTTCGATGCT 
GGCCTGCAGG CCTTCCAGCA GGAGGGCATC GCCAACATCA CTGCCCTCAA AGATCAGCTG 
CTGGCCGCCA AGCACGTTCA GTCCAAGGCC ATCGAGGCCC GCCATGCTGC CCTCATGAAG 
AGGTGGAGCC AGCTCCTGGC CAACTCGGCC ACCCGGAAGA AGAAGCTGCT GGAGGCACAG 
AGTCACTTCC GCAAGGTGGA GGATCTCTTC CTGACCTTTG CTAAAAAGGC TTCAGCCTTC 
AACAGCTGGT TTGAAAACGC AGAGGAGGAC TTAACCGACC CAGTGCGCTG CAACTCTCTG 
GAAGAGATCA AGGCCTTGAG AGAGGCCCAC GACGCCTTCC GCTCCTCGTT GAGCTCGGCC 
CAGGCCGACT TCAACCAGCT GGCAGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTGGCC 
TCCAACCCCT ACACCTGGTT CACCATGGAG GCCCTGGAGG AGACCTGGAG AAACCTACAG 
AAGATCATCA AGGAAAGGGA GCTGGAGCTG CAGAAGGAGC AGCGACGGCA GGAGGAGAAT 
GACAAGCTGC GCCAGGAGTT CGCCCAGCAT GCCAACGCCT TCCACCAGTG GATCCAGGAG 
ACCAGAACGT ACCTCCTCGA TGGCATAGCG TATCGTCGGG TCATTCGTGT CTATCAGTAT 
GAAGTTGGGG ATGATCTGTC TGGAAGGTCC TGCATGGTGG AAGAATCCGG GACCCTCGAA 
TCACAGCTTG AAGCTACCAA ACGCAAACAC CAGGAGATTC GAGCTATGAG AAGTCAGCTC 
AAGAAGATTG AAGACCTGGG GGCGGCCATG GAGGAAGCGC TGATCCTGGA CAACAAGTAC 
ACGGAGCACA GCACCGTGGG CCTGGCCCAG CAGTGGGACC AGCTCGACCA GTTGGGCATG 
CGCATGCAGC ACAACCTGGA GCAGCAGATC CAGGCCAGGA ACACGACCGG AGTGACCGAG 
GAGGCCCTCA AAGAATTCAG CATGATGTTC AAACACTTCG ACAAGGACAA GTCTGGCCGG 
CTGAACCACC AGGAGTTCAA ATCCTGTCTG CGCTCCCTGG GCTATGACCT GCCCATGGTG 
GAGGAAGGGG AGCCCGACCC TGAGTTCGAG GCCATCCTGG ACACTGTGGA TCCTAACAGG 
GACGGCCACG TCTCCCTGCA AGAGTACATG GCCTTTATGA TCAGCCGTGA GACCGAGAAC 
GTCAAGTCCA GCGAGGAGAT TGAGAGCGCC TTCCGGGCCC TGAGCTCTGA AGGGAAGCCT 
TATGTGACCA AGGAGGAGCT GTACCAGAAC CTGACTCGGG AACAAGCCGA CTACTGCGTC 
TCCCACATGA AGCCCTACGT GGACGGCAAG GGCCGCGAAC TCCCCACTGC TTTTGACTAC 
GTCGAGTTCA CCCGCTCGCT CTTCGTGAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_010810456.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010808758.2 Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa92001
Clone ID Related Accession (Same CDS sequence)	XM_010810456.2
Accession Version	XM_010810456.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7518bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810456.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGCGG GGTCAAAGTG 
CTGGAGACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTGGACAG ATACCACCGC 
TTCAAGGAGC TCTCGACCCT GAGGCGCCAG AAGCTTGAAG ACTCCTACCG ATTCCAGTTT 
TTCCAAAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAGCTTCA GATTGCCTCA 
GATGAGAATT ACAAAGACCC AACCAACCTG CAGGGCAAGC TGCAGAAGCA TCAAGCCTTC 
GAAGCCGAGG TGCAGGCCAA CTCGGGCGCC ATCGTGAAGC TGGATGAGAC TGGTAACCTG 
ATGATCTCAG AAGGGCACTT TGCATCCGAA ACCATCCGGA CTCGTCTGAT GGAGCTGCAC 
CGCCAGTGGG AATTGCTCCT GGAGAAGATG CGGGAGAAAG GCATCAAGCT GCTGCAGGCC 
CAGAAGCTGG TGCAGTACCT GCGGGAGTGT GAGGATGTGA TGGACTGGAT CAATGACAAG 
GAAGCCATTG TGACGTCTGA GGAGCTGGGC CAGGACCTGG AGCACGTGGA GGTGTTACAG 
AAGAAATTCG AAGAGTTTCA GACAGACATG GCTGCCCACG AAGAGAGAGT CAATGAAGTG 
AACCAGTTCG CTGCCAAGCT CATTCAGGAG CAGCACCCTG AGGAGGAGCT GATCAAGACA 
AAGCAGGACG AGGTGAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCCCT CCAGCGGCAG 
GGGAAGCTGT TCGGGGCAGC TGAGGTCCAA CGCTTTAACA GGGATGTGGA CGAGACCATC 
AGCTGGATTA AGGAGAAGGA ACAGTTAATG GCCTCTGATG ACTTTGGCCG GGACCTGGCC 
AGCGTCCAGG CTTTACTACG GAAACACGAG GGTTTGGAGA GAGATCTTGC TGCCTTGGAG 
GACAAGGTCA AAGCCCTGTG TGCCGAGGCT GACCGCCTGC ATCAGTCCCA CCCTCTGAGT 
GCCACCCAGA TCCAGGTGAA GCGAGAGGAG CTGATCACCA ACTGGGAGCA GATCCGCACC 
CTGGCCGCGG AGAGACACGC ACGTCTCAAC GATTCCTACA GGCTTCAGCG CTTCCTTGCC 
GACTTCCGAG ACCTCACCAG CTGGGTCACT GAGATGAAAG CCCTCATCAA CGCAGATGAA 
CTTGCCAACG ATGTGGCCGG AGCCGAAGCC CTGCTGGATA GGCATCAGGA GCACAAGGGT 
GAAATTGACG CCCATGAAGA TAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT 
GCAGCTGGTC ACTACGCCTC AGATGAAGTG AGGGAGAAGC TTACCGTGCT GTCCGAGGAG 
CGGGCCGCGC TGCTGGAGCT CTGGGAGCTG CGACGGCAGC AATATGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ACGAAGACCT GGGCGATTCC TTGGATAGTG TGGAAGCGCT TCTAAAGAAG 
CACGAAGACT TTGAGAAGTC CCTCAGCGCC CAGGAGGAGA AGATCACAGC CTTAGATGAG 
TTTGCCACCA AGCTGATTCA GAACAACCAC TACGCAATGG AAGATGTGGC CACCCGGCGA 
GACGCACTGC TGAGCCGTCG CAATGCCCTG CATGAGAGAG CCATGTACCG CCGTGCCCAG 
CTTGCAGATT CCTTCCACTT GCAGCAGTTT TTCCGTGACT CTGATGAGCT CAAGAGTTGG 
GTCAACGAGA AGATGAAAAC AGCCACGGAT GAAGCTTATA AAGACCCATC CAACCTACAA 
GGGAAAGTGC AGAAGCACCA GGCCTTTGAG GCCGAGCTCT CGGCAAACCA GAGCCGCATC 
GACGCCCTGG AGAAAGCCGG GCAGAAGCTG ATCGATGTCG ACCACTATGC CAAGGACGAG 
GTGGCGGCTC GGATGAATGA GGTCATCAGT CTGTGGAAGA AGCTGCTGGA GGCCACAGAG 
CTGAAAGGCA TAAAGCTCCG CGAGGCTAAC CAGCAGCAGC AGTTTAATCG CAACGTTGAG 
GACATCGAGC TGTGGCTGTA TGAAGTGGAA GGTCATCTGG CCTCGGACGA CTACGGCAAA 
GACCTCACCA ACGTCCAGAA CCTCCAGAAG AAGCACGCCC TGCTGGAGGC TGACGTGGCA 
GCTCACCAGG ATCGCATCGA TGGCATCACT ATCCAAGCCC GCCAGTTCCA GGACGCGGGC 
CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAGGCCC TGGTAGCTCG CTACGAGGCG 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCTG ATTCCCTGCG GTTGCAGCAG 
CTCTTCCGTG ACGTCGAGGA TGAGGAGACG TGGATTCGGG AGAAAGAACC CATTGCCGCC 
TCCACCAACA GAGGCAAAGA TTTAATTGGA GTCCAGAACC TGCTAAAGAA ACACCAAGCG 
CTACAAGCAG AAATTGCTGG CCACGAGCCC CGCATCAAAG CAGTCACACA GAAGGGGAAC 
GCCATGGTGG AGGAGGGCCA TTTTGCTGCT GAGGACGTGA AGGCCAAGCT CCATGAGCTG 
AACCAGAAGT GGGAGTCGCT GAAGTCCAAG GCCTCCCAGC GGCGGCAGGA CCTGGAGGAC 
TCGCTGCAGG CCCAGCAGTA CTTTGCCGAC GCCAACGAGG CTGAGTCCTG GATGCGGGAG 
AAGGAGCCCA TCGTGGGCAG CACCGACTAT GGCAAAGATG AGGACTCGGC CGAGGCTCTG 
CTAAAGAAGC ACGAGGCATT GATGTCGGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCC 
CTGCGAGAGC AGGCCCAGTC GTGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG 
AAGGAGCTGG TCTTGGCGCT CTATGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAAGGAG ACATTCTCAC CTTACTCAAC AGCACCAACA AGGACTGGTG GAAAGTGGAA 
GTGAATGACC GGCAGGGCTT CGTGCCGGCT GCCTACGTGA AGAAGCTGGA TCCTGCGCAG 
TCGGCCTCCC GGGAGAACCT CCTGGAAGAG CAGGGCAGCA TCGCGCTGCG GCAGGAACAG 
ATCGACAACC AGACGCGCGT AACTAAGGAG GCTGGCAGCG TGTCTCTGCG TATGAAACAG 
GTGGAAGAAC TGTACCATTC TCTGCTGGAA CTGGGAGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGCTGTA AGAAGTTCAT GTTGTTCCGT GAAGCGAACG AGCTACAGCA GTGGATCAAT 
GAGAAAGAAG CGGCCCTGAC AAGTGAGGAG GTTGGCGCGG ACTTGGAGCA GGTTGAAGTG 
CTGCAGAAGA AGTTTGACGA CTTCCAGAAG GATCTCAAGG CCAATGAGTC GCGGCTGAAG 
GACATTAACA AGGTGGCTGA AGACCTGGAG TCGGAAGGTC TGATGGCGGA GGAGGTGCAG 
GCCGTGCAGC AGCAGGAGGT GTATGGCGCG ATGCCCAGGG ATGAATCTGA TTCCAAGACA 
GCCTCCCCTT GGAAGTCTGC TCGCCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTGA ACGAGCGCTG GCGGTCCCTG CAGCAGCTGG CTGAGGAGCG GAGCCAACTC 
TTGGGCAGTG CCCATGAAGT GCAGCGGTTC CACAGAGATG CTGACGAAAC CAAAGAGTGG 
ATCGAAGAGA AGAACCAGGC TCTAAACACA GACAACTACG GCCACGACCT GGCCAGTGTC 
CAGGCCCTGC AGCGCAAGCA CGAGGGCTTC GAGAGGGACC TCGCAGCCCT TGGTGACAAG 
GTGAACTCGC TCGGGGAGAC AGCCGAGCGC CTGATCCAGT CCCACCCCGA GTCGGCAGAG 
GACCTGCAGG AGAAGTGCAC TGAGCTGAAC CAGGCATGGA GCAGCCTGGG CAAGCGTGCC 
GACCAGCGCA AGGCCAAGCT GGGCGACTCC CACGACCTGC AGCGCTTCCT CAGCGACTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATC CGAGGTCTGG TGTCCTCAGA CGAGCTAGCC 
AAGGATGTCA CCGGAGCTGA GGCCCTGCTG GAGCGACACC AGGAACACCG GACAGAAATT 
GATGCCAGAG CTGGCACTTT CCAGGCATTC GAGCAGTTTG GGCAGCAGCT GTTGGCTCAT 
GGGCACTACG CCAGCCCCGA GATCAAGGAG AAGCTCGATG TTCTAGACCA GGAGCGTGCA 
GACCTTGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAATTGCAG 
CTGTTCCATC GGGATTGCGA GCAGGCCGAG AACTGGATGG CTGCCCGAGA GGCCTTTCTG 
AACACAGAAG ACAAAGGGGA CTCGCTAGAC AGTGTGGAGG CTCTGATCAA AAAACACGAG 
GACTTTGATA AAGCAATTAA TGTCCAGGAG GAGAAGATTG CTGCCCTGCA GTCCTTTGCT 
GACCAGCTCA TCGCTGGTGG CCACTACGCC AAAGGCGACA TCTCCAGCCG GCGCAATGAG 
GTTCTGGACA GGTGGCGCCG TCTAAAAGCT CAGATGATTG AGAAGAGGTC AAAGCTGGGA 
GAATCACAGA CCCTCCAGCA GTTCAGCCGG GATGTAGACG AGATTGAAGC TTGGATCAGT 
GAAAAATTAC AGACTGCAAG TGATGAGTCA TATAAGGACC CCACCAACAT CCAGAGCAAG 
CATCAGAAGC ACCAGGCCTT CGAGGCAGAA CTGCACGCCA ATGCGGACCG GATCCGTGGG 
GTCATCGACA TGGGCAACTC CCTCATTGAC CGCGGGGCCT GCGCCGGCAG CGAGGATGCC 
GTCAAGGCCC GGCTGGCCGC CTTAGCTGAC CAGTGGCAGT TCCTGGTGCA GAAGTCGGCT 
GAGAAGAGCC AGAAGCTGAA AGAAGCCAAC AAGCAGCAGA ACTTCAACAC TGGCATCAAG 
GACTTCGACT TCTGGCTCTC TGAGGTGGAA GCCCTGCTGG CCTCTGAGGA TTATGGCAAA 
GACCTGGCTT CTGTGAACAA CTTGCTAAAA AAACATCAGC TGCTGGAGGC AGATATCTCT 
GCCCACGAGG ACCGCCTGAA GGACCTCAAC AGCCAGGCGG ACAGCCTGAT GACCAGCAGC 
GCCTTCGACA CCTCCCAGGT GAAGGACAAG CGGGACGCCA TCAACGGCCG TTTCCAGAAG 
ATCAAGAGCA TGGCCGCCTC GAGGCGAGCT AGGCTGAATG AGTCCCACCG CCTGCACCAG 
TTTTTCCGGG ACATGGATGA TGAGGAGTCC TGGATCAAGG AAAAGAAGCT GCTGGTGAGC 
TCAGAGGACT ACGGCCGTGA CCTGACGGGC GTCCAGAACC TGAGGAAGAA GCACAAACGG 
CTGGAAGCAG AGCTGGCTGC CCACGAGCCG GCCATTCAGG GTGTCCTGGA CACTGGAAAG 
AAGCTGTCTG ATGACAACAC CATCGGGAAG GAGGAGATTC AGCAGCGCCT GGCGCAGTTC 
GTGGAGCACT GGAAGGAGCT GAAGCAGCTG GCAGCTGCCC GGGGCCAGCG GCTAGAAGAA 
TCTTTGGAAT ATCAGCAGTT TGTAGCCAAC GTGGAGGAGG AGGAAGCCTG GATCAATGAG 
AAGATGACCC TGGTGGCCAG TGAAGATTAT GGAGACACTC TTGCTGCCAT CCAGGGCTTG 
CTGAAGAAAC ACGAAGCCTT CGAGACGGAC TTCACTGTCC ACAAGGACCG AGTGAATGAT 
GTCTGCACCA ACGGCCAGGA CCTGGTTAAG AAGAACAATC ACCATGAGGA GAACATCTCT 
TCCAAGATGA AGGGCTTGAA CGGGAAAGTG TCAGACTTGG AGAAGGCTGC CTCCCAGAGG 
AAGGCCAAGC TCGACGAGAA CTCGGCCTTC CTCCAGTTCA ACTGGAAAGC CGATGTGGTG 
GAGTCCTGGA TCGGTGAAAA GGAGAACAGC TTGAAGACAG ATGATTATGG TCGAGATCTG 
TCTTCTGTCC AAACTCTCCT CACCAAACAG GAAACCTTCG ATGCTGGCCT GCAGGCCTTC 
CAGCAGGAGG GCATCGCCAA CATCACTGCC CTCAAAGATC AGCTGCTGGC CGCCAAGCAC 
GTTCAGTCCA AGGCCATCGA GGCCCGCCAT GCTGCCCTCA TGAAGAGGTG GAGCCAGCTC 
CTGGCCAACT CGGCCACCCG GAAGAAGAAG CTGCTGGAGG CACAGAGTCA CTTCCGCAAG 
GTGGAGGATC TCTTCCTGAC CTTTGCTAAA AAGGCTTCAG CCTTCAACAG CTGGTTTGAA 
AACGCAGAGG AGGACTTAAC CGACCCAGTG CGCTGCAACT CTCTGGAAGA GATCAAGGCC 
TTGAGAGAGG CCCACGACGC CTTCCGCTCC TCGTTGAGCT CGGCCCAGGC CGACTTCAAC 
CAGCTGGCAG AGCTGGACCG CCAGATCAAG AGCTTCCGCG TGGCCTCCAA CCCCTACACC 
TGGTTCACCA TGGAGGCCCT GGAGGAGACC TGGAGAAACC TACAGAAGAT CATCAAGGAA 
AGGGAGCTGG AGCTGCAGAA GGAGCAGCGA CGGCAGGAGG AGAATGACAA GCTGCGCCAG 
GAGTTCGCCC AGCATGCCAA CGCCTTCCAC CAGTGGATCC AGGAGACCAG AACGTACCTC 
CTCGATGGCA TAGCGTATCG TCGGGTCATT CGTGTCTATC AGTATGAAGT TGGGGATGAT 
CTGTCTGGAA GGTCCTGCAT GGTGGAAGAA TCCGGGACCC TCGAATCACA GCTTGAAGCT 
ACCAAACGCA AACACCAGGA GATTCGAGCT ATGAGAAGTC AGCTCAAGAA GATTGAAGAC 
CTGGGGGCGG CCATGGAGGA AGCGCTGATC CTGGACAACA AGTACACGGA GCACAGCACC 
GTGGGCCTGG CCCAGCAGTG GGACCAGCTC GACCAGTTGG GCATGCGCAT GCAGCACAAC 
CTGGAGCAGC AGATCCAGGC CAGGAACACG ACCGGAGTGA CCGAGGAGGC CCTCAAAGAA 
TTCAGCATGA TGTTCAAACA CTTCGACAAG GACAAGTCTG GCCGGCTGAA CCACCAGGAG 
TTCAAATCCT GTCTGCGCTC CCTGGGCTAT GACCTGCCCA TGGTGGAGGA AGGGGAGCCC 
GACCCTGAGT TCGAGGCCAT CCTGGACACT GTGGATCCTA ACAGGGACGG CCACGTCTCC 
CTGCAAGAGT ACATGGCCTT TATGATCAGC CGTGAGACCG AGAACGTCAA GTCCAGCGAG 
GAGATTGAGA GCGCCTTCCG GGCCCTGAGC TCTGAAGGGA AGCCTTATGT GACCAAGGAG 
GAGCTGTACC AGAACCTGAC TCGGGAACAA GCCGACTACT GCGTCTCCCA CATGAAGCCC 
TACGTGGACG GCAAGGGCCG CGAACTCCCC ACTGCTTTTG ACTACGTCGA GTTCACCCGC 
TCGCTCTTCG TGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010808758.2
CDS	55..7572
Translation	MLSSFRKRRVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLHQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMYRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVDHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWESLKSKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRVTKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGAMPRDESDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKEKLDVLDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQSFADQLIAGGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQSKHQKHQAFEAELHANADRIRGVIDMGNSLIDRGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDAINGRFQKIKSMAASRRARLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLVKKNNHHEENISSKMKGLNGKVSDLEKAASQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHAALMKRWSQLLANSATRKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGIAYRRVIRVYQYEVGDDLSGRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_010810456.2
Organism	Bos taurus(cattle)
Definition	Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010810456.2

ORF Insert Sequence:

ATGCTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGCGG GGTCAAAGTG 
CTGGAGACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTGGACAG ATACCACCGC 
TTCAAGGAGC TCTCGACCCT GAGGCGCCAG AAGCTTGAAG ACTCCTACCG ATTCCAGTTT 
TTCCAAAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAGCTTCA GATTGCCTCA 
GATGAGAATT ACAAAGACCC AACCAACCTG CAGGGCAAGC TGCAGAAGCA TCAAGCCTTC 
GAAGCCGAGG TGCAGGCCAA CTCGGGCGCC ATCGTGAAGC TGGATGAGAC TGGTAACCTG 
ATGATCTCAG AAGGGCACTT TGCATCCGAA ACCATCCGGA CTCGTCTGAT GGAGCTGCAC 
CGCCAGTGGG AATTGCTCCT GGAGAAGATG CGGGAGAAAG GCATCAAGCT GCTGCAGGCC 
CAGAAGCTGG TGCAGTACCT GCGGGAGTGT GAGGATGTGA TGGACTGGAT CAATGACAAG 
GAAGCCATTG TGACGTCTGA GGAGCTGGGC CAGGACCTGG AGCACGTGGA GGTGTTACAG 
AAGAAATTCG AAGAGTTTCA GACAGACATG GCTGCCCACG AAGAGAGAGT CAATGAAGTG 
AACCAGTTCG CTGCCAAGCT CATTCAGGAG CAGCACCCTG AGGAGGAGCT GATCAAGACA 
AAGCAGGACG AGGTGAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCCCT CCAGCGGCAG 
GGGAAGCTGT TCGGGGCAGC TGAGGTCCAA CGCTTTAACA GGGATGTGGA CGAGACCATC 
AGCTGGATTA AGGAGAAGGA ACAGTTAATG GCCTCTGATG ACTTTGGCCG GGACCTGGCC 
AGCGTCCAGG CTTTACTACG GAAACACGAG GGTTTGGAGA GAGATCTTGC TGCCTTGGAG 
GACAAGGTCA AAGCCCTGTG TGCCGAGGCT GACCGCCTGC ATCAGTCCCA CCCTCTGAGT 
GCCACCCAGA TCCAGGTGAA GCGAGAGGAG CTGATCACCA ACTGGGAGCA GATCCGCACC 
CTGGCCGCGG AGAGACACGC ACGTCTCAAC GATTCCTACA GGCTTCAGCG CTTCCTTGCC 
GACTTCCGAG ACCTCACCAG CTGGGTCACT GAGATGAAAG CCCTCATCAA CGCAGATGAA 
CTTGCCAACG ATGTGGCCGG AGCCGAAGCC CTGCTGGATA GGCATCAGGA GCACAAGGGT 
GAAATTGACG CCCATGAAGA TAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT 
GCAGCTGGTC ACTACGCCTC AGATGAAGTG AGGGAGAAGC TTACCGTGCT GTCCGAGGAG 
CGGGCCGCGC TGCTGGAGCT CTGGGAGCTG CGACGGCAGC AATATGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ACGAAGACCT GGGCGATTCC TTGGATAGTG TGGAAGCGCT TCTAAAGAAG 
CACGAAGACT TTGAGAAGTC CCTCAGCGCC CAGGAGGAGA AGATCACAGC CTTAGATGAG 
TTTGCCACCA AGCTGATTCA GAACAACCAC TACGCAATGG AAGATGTGGC CACCCGGCGA 
GACGCACTGC TGAGCCGTCG CAATGCCCTG CATGAGAGAG CCATGTACCG CCGTGCCCAG 
CTTGCAGATT CCTTCCACTT GCAGCAGTTT TTCCGTGACT CTGATGAGCT CAAGAGTTGG 
GTCAACGAGA AGATGAAAAC AGCCACGGAT GAAGCTTATA AAGACCCATC CAACCTACAA 
GGGAAAGTGC AGAAGCACCA GGCCTTTGAG GCCGAGCTCT CGGCAAACCA GAGCCGCATC 
GACGCCCTGG AGAAAGCCGG GCAGAAGCTG ATCGATGTCG ACCACTATGC CAAGGACGAG 
GTGGCGGCTC GGATGAATGA GGTCATCAGT CTGTGGAAGA AGCTGCTGGA GGCCACAGAG 
CTGAAAGGCA TAAAGCTCCG CGAGGCTAAC CAGCAGCAGC AGTTTAATCG CAACGTTGAG 
GACATCGAGC TGTGGCTGTA TGAAGTGGAA GGTCATCTGG CCTCGGACGA CTACGGCAAA 
GACCTCACCA ACGTCCAGAA CCTCCAGAAG AAGCACGCCC TGCTGGAGGC TGACGTGGCA 
GCTCACCAGG ATCGCATCGA TGGCATCACT ATCCAAGCCC GCCAGTTCCA GGACGCGGGC 
CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAGGCCC TGGTAGCTCG CTACGAGGCG 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCTG ATTCCCTGCG GTTGCAGCAG 
CTCTTCCGTG ACGTCGAGGA TGAGGAGACG TGGATTCGGG AGAAAGAACC CATTGCCGCC 
TCCACCAACA GAGGCAAAGA TTTAATTGGA GTCCAGAACC TGCTAAAGAA ACACCAAGCG 
CTACAAGCAG AAATTGCTGG CCACGAGCCC CGCATCAAAG CAGTCACACA GAAGGGGAAC 
GCCATGGTGG AGGAGGGCCA TTTTGCTGCT GAGGACGTGA AGGCCAAGCT CCATGAGCTG 
AACCAGAAGT GGGAGTCGCT GAAGTCCAAG GCCTCCCAGC GGCGGCAGGA CCTGGAGGAC 
TCGCTGCAGG CCCAGCAGTA CTTTGCCGAC GCCAACGAGG CTGAGTCCTG GATGCGGGAG 
AAGGAGCCCA TCGTGGGCAG CACCGACTAT GGCAAAGATG AGGACTCGGC CGAGGCTCTG 
CTAAAGAAGC ACGAGGCATT GATGTCGGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCC 
CTGCGAGAGC AGGCCCAGTC GTGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG 
AAGGAGCTGG TCTTGGCGCT CTATGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAAGGAG ACATTCTCAC CTTACTCAAC AGCACCAACA AGGACTGGTG GAAAGTGGAA 
GTGAATGACC GGCAGGGCTT CGTGCCGGCT GCCTACGTGA AGAAGCTGGA TCCTGCGCAG 
TCGGCCTCCC GGGAGAACCT CCTGGAAGAG CAGGGCAGCA TCGCGCTGCG GCAGGAACAG 
ATCGACAACC AGACGCGCGT AACTAAGGAG GCTGGCAGCG TGTCTCTGCG TATGAAACAG 
GTGGAAGAAC TGTACCATTC TCTGCTGGAA CTGGGAGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGCTGTA AGAAGTTCAT GTTGTTCCGT GAAGCGAACG AGCTACAGCA GTGGATCAAT 
GAGAAAGAAG CGGCCCTGAC AAGTGAGGAG GTTGGCGCGG ACTTGGAGCA GGTTGAAGTG 
CTGCAGAAGA AGTTTGACGA CTTCCAGAAG GATCTCAAGG CCAATGAGTC GCGGCTGAAG 
GACATTAACA AGGTGGCTGA AGACCTGGAG TCGGAAGGTC TGATGGCGGA GGAGGTGCAG 
GCCGTGCAGC AGCAGGAGGT GTATGGCGCG ATGCCCAGGG ATGAATCTGA TTCCAAGACA 
GCCTCCCCTT GGAAGTCTGC TCGCCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTGA ACGAGCGCTG GCGGTCCCTG CAGCAGCTGG CTGAGGAGCG GAGCCAACTC 
TTGGGCAGTG CCCATGAAGT GCAGCGGTTC CACAGAGATG CTGACGAAAC CAAAGAGTGG 
ATCGAAGAGA AGAACCAGGC TCTAAACACA GACAACTACG GCCACGACCT GGCCAGTGTC 
CAGGCCCTGC AGCGCAAGCA CGAGGGCTTC GAGAGGGACC TCGCAGCCCT TGGTGACAAG 
GTGAACTCGC TCGGGGAGAC AGCCGAGCGC CTGATCCAGT CCCACCCCGA GTCGGCAGAG 
GACCTGCAGG AGAAGTGCAC TGAGCTGAAC CAGGCATGGA GCAGCCTGGG CAAGCGTGCC 
GACCAGCGCA AGGCCAAGCT GGGCGACTCC CACGACCTGC AGCGCTTCCT CAGCGACTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATC CGAGGTCTGG TGTCCTCAGA CGAGCTAGCC 
AAGGATGTCA CCGGAGCTGA GGCCCTGCTG GAGCGACACC AGGAACACCG GACAGAAATT 
GATGCCAGAG CTGGCACTTT CCAGGCATTC GAGCAGTTTG GGCAGCAGCT GTTGGCTCAT 
GGGCACTACG CCAGCCCCGA GATCAAGGAG AAGCTCGATG TTCTAGACCA GGAGCGTGCA 
GACCTTGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAATTGCAG 
CTGTTCCATC GGGATTGCGA GCAGGCCGAG AACTGGATGG CTGCCCGAGA GGCCTTTCTG 
AACACAGAAG ACAAAGGGGA CTCGCTAGAC AGTGTGGAGG CTCTGATCAA AAAACACGAG 
GACTTTGATA AAGCAATTAA TGTCCAGGAG GAGAAGATTG CTGCCCTGCA GTCCTTTGCT 
GACCAGCTCA TCGCTGGTGG CCACTACGCC AAAGGCGACA TCTCCAGCCG GCGCAATGAG 
GTTCTGGACA GGTGGCGCCG TCTAAAAGCT CAGATGATTG AGAAGAGGTC AAAGCTGGGA 
GAATCACAGA CCCTCCAGCA GTTCAGCCGG GATGTAGACG AGATTGAAGC TTGGATCAGT 
GAAAAATTAC AGACTGCAAG TGATGAGTCA TATAAGGACC CCACCAACAT CCAGAGCAAG 
CATCAGAAGC ACCAGGCCTT CGAGGCAGAA CTGCACGCCA ATGCGGACCG GATCCGTGGG 
GTCATCGACA TGGGCAACTC CCTCATTGAC CGCGGGGCCT GCGCCGGCAG CGAGGATGCC 
GTCAAGGCCC GGCTGGCCGC CTTAGCTGAC CAGTGGCAGT TCCTGGTGCA GAAGTCGGCT 
GAGAAGAGCC AGAAGCTGAA AGAAGCCAAC AAGCAGCAGA ACTTCAACAC TGGCATCAAG 
GACTTCGACT TCTGGCTCTC TGAGGTGGAA GCCCTGCTGG CCTCTGAGGA TTATGGCAAA 
GACCTGGCTT CTGTGAACAA CTTGCTAAAA AAACATCAGC TGCTGGAGGC AGATATCTCT 
GCCCACGAGG ACCGCCTGAA GGACCTCAAC AGCCAGGCGG ACAGCCTGAT GACCAGCAGC 
GCCTTCGACA CCTCCCAGGT GAAGGACAAG CGGGACGCCA TCAACGGCCG TTTCCAGAAG 
ATCAAGAGCA TGGCCGCCTC GAGGCGAGCT AGGCTGAATG AGTCCCACCG CCTGCACCAG 
TTTTTCCGGG ACATGGATGA TGAGGAGTCC TGGATCAAGG AAAAGAAGCT GCTGGTGAGC 
TCAGAGGACT ACGGCCGTGA CCTGACGGGC GTCCAGAACC TGAGGAAGAA GCACAAACGG 
CTGGAAGCAG AGCTGGCTGC CCACGAGCCG GCCATTCAGG GTGTCCTGGA CACTGGAAAG 
AAGCTGTCTG ATGACAACAC CATCGGGAAG GAGGAGATTC AGCAGCGCCT GGCGCAGTTC 
GTGGAGCACT GGAAGGAGCT GAAGCAGCTG GCAGCTGCCC GGGGCCAGCG GCTAGAAGAA 
TCTTTGGAAT ATCAGCAGTT TGTAGCCAAC GTGGAGGAGG AGGAAGCCTG GATCAATGAG 
AAGATGACCC TGGTGGCCAG TGAAGATTAT GGAGACACTC TTGCTGCCAT CCAGGGCTTG 
CTGAAGAAAC ACGAAGCCTT CGAGACGGAC TTCACTGTCC ACAAGGACCG AGTGAATGAT 
GTCTGCACCA ACGGCCAGGA CCTGGTTAAG AAGAACAATC ACCATGAGGA GAACATCTCT 
TCCAAGATGA AGGGCTTGAA CGGGAAAGTG TCAGACTTGG AGAAGGCTGC CTCCCAGAGG 
AAGGCCAAGC TCGACGAGAA CTCGGCCTTC CTCCAGTTCA ACTGGAAAGC CGATGTGGTG 
GAGTCCTGGA TCGGTGAAAA GGAGAACAGC TTGAAGACAG ATGATTATGG TCGAGATCTG 
TCTTCTGTCC AAACTCTCCT CACCAAACAG GAAACCTTCG ATGCTGGCCT GCAGGCCTTC 
CAGCAGGAGG GCATCGCCAA CATCACTGCC CTCAAAGATC AGCTGCTGGC CGCCAAGCAC 
GTTCAGTCCA AGGCCATCGA GGCCCGCCAT GCTGCCCTCA TGAAGAGGTG GAGCCAGCTC 
CTGGCCAACT CGGCCACCCG GAAGAAGAAG CTGCTGGAGG CACAGAGTCA CTTCCGCAAG 
GTGGAGGATC TCTTCCTGAC CTTTGCTAAA AAGGCTTCAG CCTTCAACAG CTGGTTTGAA 
AACGCAGAGG AGGACTTAAC CGACCCAGTG CGCTGCAACT CTCTGGAAGA GATCAAGGCC 
TTGAGAGAGG CCCACGACGC CTTCCGCTCC TCGTTGAGCT CGGCCCAGGC CGACTTCAAC 
CAGCTGGCAG AGCTGGACCG CCAGATCAAG AGCTTCCGCG TGGCCTCCAA CCCCTACACC 
TGGTTCACCA TGGAGGCCCT GGAGGAGACC TGGAGAAACC TACAGAAGAT CATCAAGGAA 
AGGGAGCTGG AGCTGCAGAA GGAGCAGCGA CGGCAGGAGG AGAATGACAA GCTGCGCCAG 
GAGTTCGCCC AGCATGCCAA CGCCTTCCAC CAGTGGATCC AGGAGACCAG AACGTACCTC 
CTCGATGGCA TAGCGTATCG TCGGGTCATT CGTGTCTATC AGTATGAAGT TGGGGATGAT 
CTGTCTGGAA GGTCCTGCAT GGTGGAAGAA TCCGGGACCC TCGAATCACA GCTTGAAGCT 
ACCAAACGCA AACACCAGGA GATTCGAGCT ATGAGAAGTC AGCTCAAGAA GATTGAAGAC 
CTGGGGGCGG CCATGGAGGA AGCGCTGATC CTGGACAACA AGTACACGGA GCACAGCACC 
GTGGGCCTGG CCCAGCAGTG GGACCAGCTC GACCAGTTGG GCATGCGCAT GCAGCACAAC 
CTGGAGCAGC AGATCCAGGC CAGGAACACG ACCGGAGTGA CCGAGGAGGC CCTCAAAGAA 
TTCAGCATGA TGTTCAAACA CTTCGACAAG GACAAGTCTG GCCGGCTGAA CCACCAGGAG 
TTCAAATCCT GTCTGCGCTC CCTGGGCTAT GACCTGCCCA TGGTGGAGGA AGGGGAGCCC 
GACCCTGAGT TCGAGGCCAT CCTGGACACT GTGGATCCTA ACAGGGACGG CCACGTCTCC 
CTGCAAGAGT ACATGGCCTT TATGATCAGC CGTGAGACCG AGAACGTCAA GTCCAGCGAG 
GAGATTGAGA GCGCCTTCCG GGCCCTGAGC TCTGAAGGGA AGCCTTATGT GACCAAGGAG 
GAGCTGTACC AGAACCTGAC TCGGGAACAA GCCGACTACT GCGTCTCCCA CATGAAGCCC 
TACGTGGACG GCAAGGGCCG CGAACTCCCC ACTGCTTTTG ACTACGTCGA GTTCACCCGC 
TCGCTCTTCG TGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_010810457.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010808759.2 Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa92002
Clone ID Related Accession (Same CDS sequence)	XM_010810457.2
Accession Version	XM_010810457.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7485bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810457.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGCGG GGTCAAAGTG 
CTGGAGACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTGGACAG ATACCACCGC 
TTCAAGGAGC TCTCGACCCT GAGGCGCCAG AAGCTTGAAG ACTCCTACCG ATTCCAGTTT 
TTCCAAAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAGCTTCA GATTGCCTCA 
GATGAGAATT ACAAAGACCC AACCAACCTG CAGGGCAAGC TGCAGAAGCA TCAAGCCTTC 
GAAGCCGAGG TGCAGGCCAA CTCGGGCGCC ATCGTGAAGC TGGATGAGAC TGGTAACCTG 
ATGATCTCAG AAGGGCACTT TGCATCCGAA ACCATCCGGA CTCGTCTGAT GGAGCTGCAC 
CGCCAGTGGG AATTGCTCCT GGAGAAGATG CGGGAGAAAG GCATCAAGCT GCTGCAGGCC 
CAGAAGCTGG TGCAGTACCT GCGGGAGTGT GAGGATGTGA TGGACTGGAT CAATGACAAG 
GAAGCCATTG TGACGTCTGA GGAGCTGGGC CAGGACCTGG AGCACGTGGA GGTGTTACAG 
AAGAAATTCG AAGAGTTTCA GACAGACATG GCTGCCCACG AAGAGAGAGT CAATGAAGTG 
AACCAGTTCG CTGCCAAGCT CATTCAGGAG CAGCACCCTG AGGAGGAGCT GATCAAGACA 
AAGCAGGACG AGGTGAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCCCT CCAGCGGCAG 
GGGAAGCTGT TCGGGGCAGC TGAGGTCCAA CGCTTTAACA GGGATGTGGA CGAGACCATC 
AGCTGGATTA AGGAGAAGGA ACAGTTAATG GCCTCTGATG ACTTTGGCCG GGACCTGGCC 
AGCGTCCAGG CTTTACTACG GAAACACGAG GGTTTGGAGA GAGATCTTGC TGCCTTGGAG 
GACAAGGTCA AAGCCCTGTG TGCCGAGGCT GACCGCCTGC ATCAGTCCCA CCCTCTGAGT 
GCCACCCAGA TCCAGGTGAA GCGAGAGGAG CTGATCACCA ACTGGGAGCA GATCCGCACC 
CTGGCCGCGG AGAGACACGC ACGTCTCAAC GATTCCTACA GGCTTCAGCG CTTCCTTGCC 
GACTTCCGAG ACCTCACCAG CTGGGTCACT GAGATGAAAG CCCTCATCAA CGCAGATGAA 
CTTGCCAACG ATGTGGCCGG AGCCGAAGCC CTGCTGGATA GGCATCAGGA GCACAAGGGT 
GAAATTGACG CCCATGAAGA TAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT 
GCAGCTGGTC ACTACGCCTC AGATGAAGTG AGGGAGAAGC TTACCGTGCT GTCCGAGGAG 
CGGGCCGCGC TGCTGGAGCT CTGGGAGCTG CGACGGCAGC AATATGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ACGAAGACCT GGGCGATTCC TTGGATAGTG TGGAAGCGCT TCTAAAGAAG 
CACGAAGACT TTGAGAAGTC CCTCAGCGCC CAGGAGGAGA AGATCACAGC CTTAGATGAG 
TTTGCCACCA AGCTGATTCA GAACAACCAC TACGCAATGG AAGATGTGGC CACCCGGCGA 
GACGCACTGC TGAGCCGTCG CAATGCCCTG CATGAGAGAG CCATGTACCG CCGTGCCCAG 
CTTGCAGATT CCTTCCACTT GCAGCAGTTT TTCCGTGACT CTGATGAGCT CAAGAGTTGG 
GTCAACGAGA AGATGAAAAC AGCCACGGAT GAAGCTTATA AAGACCCATC CAACCTACAA 
GGGAAAGTGC AGAAGCACCA GGCCTTTGAG GCCGAGCTCT CGGCAAACCA GAGCCGCATC 
GACGCCCTGG AGAAAGCCGG GCAGAAGCTG ATCGATGTCG ACCACTATGC CAAGGACGAG 
GTGGCGGCTC GGATGAATGA GGTCATCAGT CTGTGGAAGA AGCTGCTGGA GGCCACAGAG 
CTGAAAGGCA TAAAGCTCCG CGAGGCTAAC CAGCAGCAGC AGTTTAATCG CAACGTTGAG 
GACATCGAGC TGTGGCTGTA TGAAGTGGAA GGTCATCTGG CCTCGGACGA CTACGGCAAA 
GACCTCACCA ACGTCCAGAA CCTCCAGAAG AAGCACGCCC TGCTGGAGGC TGACGTGGCA 
GCTCACCAGG ATCGCATCGA TGGCATCACT ATCCAAGCCC GCCAGTTCCA GGACGCGGGC 
CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAGGCCC TGGTAGCTCG CTACGAGGCG 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCTG ATTCCCTGCG GTTGCAGCAG 
CTCTTCCGTG ACGTCGAGGA TGAGGAGACG TGGATTCGGG AGAAAGAACC CATTGCCGCC 
TCCACCAACA GAGGCAAAGA TTTAATTGGA GTCCAGAACC TGCTAAAGAA ACACCAAGCG 
CTACAAGCAG AAATTGCTGG CCACGAGCCC CGCATCAAAG CAGTCACACA GAAGGGGAAC 
GCCATGGTGG AGGAGGGCCA TTTTGCTGCT GAGGACGTGA AGGCCAAGCT CCATGAGCTG 
AACCAGAAGT GGGAGTCGCT GAAGTCCAAG GCCTCCCAGC GGCGGCAGGA CCTGGAGGAC 
TCGCTGCAGG CCCAGCAGTA CTTTGCCGAC GCCAACGAGG CTGAGTCCTG GATGCGGGAG 
AAGGAGCCCA TCGTGGGCAG CACCGACTAT GGCAAAGATG AGGACTCGGC CGAGGCTCTG 
CTAAAGAAGC ACGAGGCATT GATGTCGGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCC 
CTGCGAGAGC AGGCCCAGTC GTGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG 
AAGGAGCTGG TCTTGGCGCT CTATGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAAGGAG ACATTCTCAC CTTACTCAAC AGCACCAACA AGGACTGGTG GAAAGTGGAA 
GTGAATGACC GGCAGGGCTT CGTGCCGGCT GCCTACGTGA AGAAGCTGGA TCCTGCGCAG 
TCGGCCTCCC GGGAGAACCT CCTGGAAGAG CAGGGCAGCA TCGCGCTGCG GCAGGAACAG 
ATCGACAACC AGACGCGCGT AACTAAGGAG GCTGGCAGCG TGTCTCTGCG TATGAAACAG 
GTGGAAGAAC TGTACCATTC TCTGCTGGAA CTGGGAGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGCTGTA AGAAGTTCAT GTTGTTCCGT GAAGCGAACG AGCTACAGCA GTGGATCAAT 
GAGAAAGAAG CGGCCCTGAC AAGTGAGGAG GTTGGCGCGG ACTTGGAGCA GGTTGAAGTG 
CTGCAGAAGA AGTTTGACGA CTTCCAGAAG GATCTCAAGG CCAATGAGTC GCGGCTGAAG 
GACATTAACA AGGTGGCTGA AGACCTGGAG TCGGAAGGTC TGATGGCGGA GGAGGTGCAG 
GCCGTGCAGC AGCAGGAGGT GTATGGCGCG ATGCCCAGGG ATGAATCTGA TTCCAAGACA 
GCCTCCCCTT GGAAGGAGCT GAACGAGCGC TGGCGGTCCC TGCAGCAGCT GGCTGAGGAG 
CGGAGCCAAC TCTTGGGCAG TGCCCATGAA GTGCAGCGGT TCCACAGAGA TGCTGACGAA 
ACCAAAGAGT GGATCGAAGA GAAGAACCAG GCTCTAAACA CAGACAACTA CGGCCACGAC 
CTGGCCAGTG TCCAGGCCCT GCAGCGCAAG CACGAGGGCT TCGAGAGGGA CCTCGCAGCC 
CTTGGTGACA AGGTGAACTC GCTCGGGGAG ACAGCCGAGC GCCTGATCCA GTCCCACCCC 
GAGTCGGCAG AGGACCTGCA GGAGAAGTGC ACTGAGCTGA ACCAGGCATG GAGCAGCCTG 
GGCAAGCGTG CCGACCAGCG CAAGGCCAAG CTGGGCGACT CCCACGACCT GCAGCGCTTC 
CTCAGCGACT TCCGGGACCT CATGTCTTGG ATCAATGGAA TCCGAGGTCT GGTGTCCTCA 
GACGAGCTAG CCAAGGATGT CACCGGAGCT GAGGCCCTGC TGGAGCGACA CCAGGAACAC 
CGGACAGAAA TTGATGCCAG AGCTGGCACT TTCCAGGCAT TCGAGCAGTT TGGGCAGCAG 
CTGTTGGCTC ATGGGCACTA CGCCAGCCCC GAGATCAAGG AGAAGCTCGA TGTTCTAGAC 
CAGGAGCGTG CAGACCTTGA GAAGGCCTGG GTCCAGCGCA GGATGATGCT GGATCAGTGC 
CTTGAATTGC AGCTGTTCCA TCGGGATTGC GAGCAGGCCG AGAACTGGAT GGCTGCCCGA 
GAGGCCTTTC TGAACACAGA AGACAAAGGG GACTCGCTAG ACAGTGTGGA GGCTCTGATC 
AAAAAACACG AGGACTTTGA TAAAGCAATT AATGTCCAGG AGGAGAAGAT TGCTGCCCTG 
CAGTCCTTTG CTGACCAGCT CATCGCTGGT GGCCACTACG CCAAAGGCGA CATCTCCAGC 
CGGCGCAATG AGGTTCTGGA CAGGTGGCGC CGTCTAAAAG CTCAGATGAT TGAGAAGAGG 
TCAAAGCTGG GAGAATCACA GACCCTCCAG CAGTTCAGCC GGGATGTAGA CGAGATTGAA 
GCTTGGATCA GTGAAAAATT ACAGACTGCA AGTGATGAGT CATATAAGGA CCCCACCAAC 
ATCCAGCTTT CCAAGCTGCT GAGCAAGCAT CAGAAGCACC AGGCCTTCGA GGCAGAACTG 
CACGCCAATG CGGACCGGAT CCGTGGGGTC ATCGACATGG GCAACTCCCT CATTGACCGC 
GGGGCCTGCG CCGGCAGCGA GGATGCCGTC AAGGCCCGGC TGGCCGCCTT AGCTGACCAG 
TGGCAGTTCC TGGTGCAGAA GTCGGCTGAG AAGAGCCAGA AGCTGAAAGA AGCCAACAAG 
CAGCAGAACT TCAACACTGG CATCAAGGAC TTCGACTTCT GGCTCTCTGA GGTGGAAGCC 
CTGCTGGCCT CTGAGGATTA TGGCAAAGAC CTGGCTTCTG TGAACAACTT GCTAAAAAAA 
CATCAGCTGC TGGAGGCAGA TATCTCTGCC CACGAGGACC GCCTGAAGGA CCTCAACAGC 
CAGGCGGACA GCCTGATGAC CAGCAGCGCC TTCGACACCT CCCAGGTGAA GGACAAGCGG 
GACGCCATCA ACGGCCGTTT CCAGAAGATC AAGAGCATGG CCGCCTCGAG GCGAGCTAGG 
CTGAATGAGT CCCACCGCCT GCACCAGTTT TTCCGGGACA TGGATGATGA GGAGTCCTGG 
ATCAAGGAAA AGAAGCTGCT GGTGAGCTCA GAGGACTACG GCCGTGACCT GACGGGCGTC 
CAGAACCTGA GGAAGAAGCA CAAACGGCTG GAAGCAGAGC TGGCTGCCCA CGAGCCGGCC 
ATTCAGGGTG TCCTGGACAC TGGAAAGAAG CTGTCTGATG ACAACACCAT CGGGAAGGAG 
GAGATTCAGC AGCGCCTGGC GCAGTTCGTG GAGCACTGGA AGGAGCTGAA GCAGCTGGCA 
GCTGCCCGGG GCCAGCGGCT AGAAGAATCT TTGGAATATC AGCAGTTTGT AGCCAACGTG 
GAGGAGGAGG AAGCCTGGAT CAATGAGAAG ATGACCCTGG TGGCCAGTGA AGATTATGGA 
GACACTCTTG CTGCCATCCA GGGCTTGCTG AAGAAACACG AAGCCTTCGA GACGGACTTC 
ACTGTCCACA AGGACCGAGT GAATGATGTC TGCACCAACG GCCAGGACCT GGTTAAGAAG 
AACAATCACC ATGAGGAGAA CATCTCTTCC AAGATGAAGG GCTTGAACGG GAAAGTGTCA 
GACTTGGAGA AGGCTGCCTC CCAGAGGAAG GCCAAGCTCG ACGAGAACTC GGCCTTCCTC 
CAGTTCAACT GGAAAGCCGA TGTGGTGGAG TCCTGGATCG GTGAAAAGGA GAACAGCTTG 
AAGACAGATG ATTATGGTCG AGATCTGTCT TCTGTCCAAA CTCTCCTCAC CAAACAGGAA 
ACCTTCGATG CTGGCCTGCA GGCCTTCCAG CAGGAGGGCA TCGCCAACAT CACTGCCCTC 
AAAGATCAGC TGCTGGCCGC CAAGCACGTT CAGTCCAAGG CCATCGAGGC CCGCCATGCT 
GCCCTCATGA AGAGGTGGAG CCAGCTCCTG GCCAACTCGG CCACCCGGAA GAAGAAGCTG 
CTGGAGGCAC AGAGTCACTT CCGCAAGGTG GAGGATCTCT TCCTGACCTT TGCTAAAAAG 
GCTTCAGCCT TCAACAGCTG GTTTGAAAAC GCAGAGGAGG ACTTAACCGA CCCAGTGCGC 
TGCAACTCTC TGGAAGAGAT CAAGGCCTTG AGAGAGGCCC ACGACGCCTT CCGCTCCTCG 
TTGAGCTCGG CCCAGGCCGA CTTCAACCAG CTGGCAGAGC TGGACCGCCA GATCAAGAGC 
TTCCGCGTGG CCTCCAACCC CTACACCTGG TTCACCATGG AGGCCCTGGA GGAGACCTGG 
AGAAACCTAC AGAAGATCAT CAAGGAAAGG GAGCTGGAGC TGCAGAAGGA GCAGCGACGG 
CAGGAGGAGA ATGACAAGCT GCGCCAGGAG TTCGCCCAGC ATGCCAACGC CTTCCACCAG 
TGGATCCAGG AGACCAGAAC GTACCTCCTC GATGGCATAG CGTATCGTCG GGTCATTCGT 
GTCTATCAGT ATGAAGTTGG GGATGATCTG TCTGGAAGGT CCTGCATGGT GGAAGAATCC 
GGGACCCTCG AATCACAGCT TGAAGCTACC AAACGCAAAC ACCAGGAGAT TCGAGCTATG 
AGAAGTCAGC TCAAGAAGAT TGAAGACCTG GGGGCGGCCA TGGAGGAAGC GCTGATCCTG 
GACAACAAGT ACACGGAGCA CAGCACCGTG GGCCTGGCCC AGCAGTGGGA CCAGCTCGAC 
CAGTTGGGCA TGCGCATGCA GCACAACCTG GAGCAGCAGA TCCAGGCCAG GAACACGACC 
GGAGTGACCG AGGAGGCCCT CAAAGAATTC AGCATGATGT TCAAACACTT CGACAAGGAC 
AAGTCTGGCC GGCTGAACCA CCAGGAGTTC AAATCCTGTC TGCGCTCCCT GGGCTATGAC 
CTGCCCATGG TGGAGGAAGG GGAGCCCGAC CCTGAGTTCG AGGCCATCCT GGACACTGTG 
GATCCTAACA GGGACGGCCA CGTCTCCCTG CAAGAGTACA TGGCCTTTAT GATCAGCCGT 
GAGACCGAGA ACGTCAAGTC CAGCGAGGAG ATTGAGAGCG CCTTCCGGGC CCTGAGCTCT 
GAAGGGAAGC CTTATGTGAC CAAGGAGGAG CTGTACCAGA ACCTGACTCG GGAACAAGCC 
GACTACTGCG TCTCCCACAT GAAGCCCTAC GTGGACGGCA AGGGCCGCGA ACTCCCCACT 
GCTTTTGACT ACGTCGAGTT CACCCGCTCG CTCTTCGTGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010808759.2
CDS	55..7539
Translation	MLSSFRKRRVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLHQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMYRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVDHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWESLKSKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRVTKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGAMPRDESDSKTASPWKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKEKLDVLDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQSFADQLIAGGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDMGNSLIDRGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDAINGRFQKIKSMAASRRARLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLVKKNNHHEENISSKMKGLNGKVSDLEKAASQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHAALMKRWSQLLANSATRKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGIAYRRVIRVYQYEVGDDLSGRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_010810457.2
Organism	Bos taurus(cattle)
Definition	Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010810457.2

ORF Insert Sequence:

ATGCTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGCGG GGTCAAAGTG 
CTGGAGACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTGGACAG ATACCACCGC 
TTCAAGGAGC TCTCGACCCT GAGGCGCCAG AAGCTTGAAG ACTCCTACCG ATTCCAGTTT 
TTCCAAAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAGCTTCA GATTGCCTCA 
GATGAGAATT ACAAAGACCC AACCAACCTG CAGGGCAAGC TGCAGAAGCA TCAAGCCTTC 
GAAGCCGAGG TGCAGGCCAA CTCGGGCGCC ATCGTGAAGC TGGATGAGAC TGGTAACCTG 
ATGATCTCAG AAGGGCACTT TGCATCCGAA ACCATCCGGA CTCGTCTGAT GGAGCTGCAC 
CGCCAGTGGG AATTGCTCCT GGAGAAGATG CGGGAGAAAG GCATCAAGCT GCTGCAGGCC 
CAGAAGCTGG TGCAGTACCT GCGGGAGTGT GAGGATGTGA TGGACTGGAT CAATGACAAG 
GAAGCCATTG TGACGTCTGA GGAGCTGGGC CAGGACCTGG AGCACGTGGA GGTGTTACAG 
AAGAAATTCG AAGAGTTTCA GACAGACATG GCTGCCCACG AAGAGAGAGT CAATGAAGTG 
AACCAGTTCG CTGCCAAGCT CATTCAGGAG CAGCACCCTG AGGAGGAGCT GATCAAGACA 
AAGCAGGACG AGGTGAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCCCT CCAGCGGCAG 
GGGAAGCTGT TCGGGGCAGC TGAGGTCCAA CGCTTTAACA GGGATGTGGA CGAGACCATC 
AGCTGGATTA AGGAGAAGGA ACAGTTAATG GCCTCTGATG ACTTTGGCCG GGACCTGGCC 
AGCGTCCAGG CTTTACTACG GAAACACGAG GGTTTGGAGA GAGATCTTGC TGCCTTGGAG 
GACAAGGTCA AAGCCCTGTG TGCCGAGGCT GACCGCCTGC ATCAGTCCCA CCCTCTGAGT 
GCCACCCAGA TCCAGGTGAA GCGAGAGGAG CTGATCACCA ACTGGGAGCA GATCCGCACC 
CTGGCCGCGG AGAGACACGC ACGTCTCAAC GATTCCTACA GGCTTCAGCG CTTCCTTGCC 
GACTTCCGAG ACCTCACCAG CTGGGTCACT GAGATGAAAG CCCTCATCAA CGCAGATGAA 
CTTGCCAACG ATGTGGCCGG AGCCGAAGCC CTGCTGGATA GGCATCAGGA GCACAAGGGT 
GAAATTGACG CCCATGAAGA TAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT 
GCAGCTGGTC ACTACGCCTC AGATGAAGTG AGGGAGAAGC TTACCGTGCT GTCCGAGGAG 
CGGGCCGCGC TGCTGGAGCT CTGGGAGCTG CGACGGCAGC AATATGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ACGAAGACCT GGGCGATTCC TTGGATAGTG TGGAAGCGCT TCTAAAGAAG 
CACGAAGACT TTGAGAAGTC CCTCAGCGCC CAGGAGGAGA AGATCACAGC CTTAGATGAG 
TTTGCCACCA AGCTGATTCA GAACAACCAC TACGCAATGG AAGATGTGGC CACCCGGCGA 
GACGCACTGC TGAGCCGTCG CAATGCCCTG CATGAGAGAG CCATGTACCG CCGTGCCCAG 
CTTGCAGATT CCTTCCACTT GCAGCAGTTT TTCCGTGACT CTGATGAGCT CAAGAGTTGG 
GTCAACGAGA AGATGAAAAC AGCCACGGAT GAAGCTTATA AAGACCCATC CAACCTACAA 
GGGAAAGTGC AGAAGCACCA GGCCTTTGAG GCCGAGCTCT CGGCAAACCA GAGCCGCATC 
GACGCCCTGG AGAAAGCCGG GCAGAAGCTG ATCGATGTCG ACCACTATGC CAAGGACGAG 
GTGGCGGCTC GGATGAATGA GGTCATCAGT CTGTGGAAGA AGCTGCTGGA GGCCACAGAG 
CTGAAAGGCA TAAAGCTCCG CGAGGCTAAC CAGCAGCAGC AGTTTAATCG CAACGTTGAG 
GACATCGAGC TGTGGCTGTA TGAAGTGGAA GGTCATCTGG CCTCGGACGA CTACGGCAAA 
GACCTCACCA ACGTCCAGAA CCTCCAGAAG AAGCACGCCC TGCTGGAGGC TGACGTGGCA 
GCTCACCAGG ATCGCATCGA TGGCATCACT ATCCAAGCCC GCCAGTTCCA GGACGCGGGC 
CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAGGCCC TGGTAGCTCG CTACGAGGCG 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCTG ATTCCCTGCG GTTGCAGCAG 
CTCTTCCGTG ACGTCGAGGA TGAGGAGACG TGGATTCGGG AGAAAGAACC CATTGCCGCC 
TCCACCAACA GAGGCAAAGA TTTAATTGGA GTCCAGAACC TGCTAAAGAA ACACCAAGCG 
CTACAAGCAG AAATTGCTGG CCACGAGCCC CGCATCAAAG CAGTCACACA GAAGGGGAAC 
GCCATGGTGG AGGAGGGCCA TTTTGCTGCT GAGGACGTGA AGGCCAAGCT CCATGAGCTG 
AACCAGAAGT GGGAGTCGCT GAAGTCCAAG GCCTCCCAGC GGCGGCAGGA CCTGGAGGAC 
TCGCTGCAGG CCCAGCAGTA CTTTGCCGAC GCCAACGAGG CTGAGTCCTG GATGCGGGAG 
AAGGAGCCCA TCGTGGGCAG CACCGACTAT GGCAAAGATG AGGACTCGGC CGAGGCTCTG 
CTAAAGAAGC ACGAGGCATT GATGTCGGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCC 
CTGCGAGAGC AGGCCCAGTC GTGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG 
AAGGAGCTGG TCTTGGCGCT CTATGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAAGGAG ACATTCTCAC CTTACTCAAC AGCACCAACA AGGACTGGTG GAAAGTGGAA 
GTGAATGACC GGCAGGGCTT CGTGCCGGCT GCCTACGTGA AGAAGCTGGA TCCTGCGCAG 
TCGGCCTCCC GGGAGAACCT CCTGGAAGAG CAGGGCAGCA TCGCGCTGCG GCAGGAACAG 
ATCGACAACC AGACGCGCGT AACTAAGGAG GCTGGCAGCG TGTCTCTGCG TATGAAACAG 
GTGGAAGAAC TGTACCATTC TCTGCTGGAA CTGGGAGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGCTGTA AGAAGTTCAT GTTGTTCCGT GAAGCGAACG AGCTACAGCA GTGGATCAAT 
GAGAAAGAAG CGGCCCTGAC AAGTGAGGAG GTTGGCGCGG ACTTGGAGCA GGTTGAAGTG 
CTGCAGAAGA AGTTTGACGA CTTCCAGAAG GATCTCAAGG CCAATGAGTC GCGGCTGAAG 
GACATTAACA AGGTGGCTGA AGACCTGGAG TCGGAAGGTC TGATGGCGGA GGAGGTGCAG 
GCCGTGCAGC AGCAGGAGGT GTATGGCGCG ATGCCCAGGG ATGAATCTGA TTCCAAGACA 
GCCTCCCCTT GGAAGGAGCT GAACGAGCGC TGGCGGTCCC TGCAGCAGCT GGCTGAGGAG 
CGGAGCCAAC TCTTGGGCAG TGCCCATGAA GTGCAGCGGT TCCACAGAGA TGCTGACGAA 
ACCAAAGAGT GGATCGAAGA GAAGAACCAG GCTCTAAACA CAGACAACTA CGGCCACGAC 
CTGGCCAGTG TCCAGGCCCT GCAGCGCAAG CACGAGGGCT TCGAGAGGGA CCTCGCAGCC 
CTTGGTGACA AGGTGAACTC GCTCGGGGAG ACAGCCGAGC GCCTGATCCA GTCCCACCCC 
GAGTCGGCAG AGGACCTGCA GGAGAAGTGC ACTGAGCTGA ACCAGGCATG GAGCAGCCTG 
GGCAAGCGTG CCGACCAGCG CAAGGCCAAG CTGGGCGACT CCCACGACCT GCAGCGCTTC 
CTCAGCGACT TCCGGGACCT CATGTCTTGG ATCAATGGAA TCCGAGGTCT GGTGTCCTCA 
GACGAGCTAG CCAAGGATGT CACCGGAGCT GAGGCCCTGC TGGAGCGACA CCAGGAACAC 
CGGACAGAAA TTGATGCCAG AGCTGGCACT TTCCAGGCAT TCGAGCAGTT TGGGCAGCAG 
CTGTTGGCTC ATGGGCACTA CGCCAGCCCC GAGATCAAGG AGAAGCTCGA TGTTCTAGAC 
CAGGAGCGTG CAGACCTTGA GAAGGCCTGG GTCCAGCGCA GGATGATGCT GGATCAGTGC 
CTTGAATTGC AGCTGTTCCA TCGGGATTGC GAGCAGGCCG AGAACTGGAT GGCTGCCCGA 
GAGGCCTTTC TGAACACAGA AGACAAAGGG GACTCGCTAG ACAGTGTGGA GGCTCTGATC 
AAAAAACACG AGGACTTTGA TAAAGCAATT AATGTCCAGG AGGAGAAGAT TGCTGCCCTG 
CAGTCCTTTG CTGACCAGCT CATCGCTGGT GGCCACTACG CCAAAGGCGA CATCTCCAGC 
CGGCGCAATG AGGTTCTGGA CAGGTGGCGC CGTCTAAAAG CTCAGATGAT TGAGAAGAGG 
TCAAAGCTGG GAGAATCACA GACCCTCCAG CAGTTCAGCC GGGATGTAGA CGAGATTGAA 
GCTTGGATCA GTGAAAAATT ACAGACTGCA AGTGATGAGT CATATAAGGA CCCCACCAAC 
ATCCAGCTTT CCAAGCTGCT GAGCAAGCAT CAGAAGCACC AGGCCTTCGA GGCAGAACTG 
CACGCCAATG CGGACCGGAT CCGTGGGGTC ATCGACATGG GCAACTCCCT CATTGACCGC 
GGGGCCTGCG CCGGCAGCGA GGATGCCGTC AAGGCCCGGC TGGCCGCCTT AGCTGACCAG 
TGGCAGTTCC TGGTGCAGAA GTCGGCTGAG AAGAGCCAGA AGCTGAAAGA AGCCAACAAG 
CAGCAGAACT TCAACACTGG CATCAAGGAC TTCGACTTCT GGCTCTCTGA GGTGGAAGCC 
CTGCTGGCCT CTGAGGATTA TGGCAAAGAC CTGGCTTCTG TGAACAACTT GCTAAAAAAA 
CATCAGCTGC TGGAGGCAGA TATCTCTGCC CACGAGGACC GCCTGAAGGA CCTCAACAGC 
CAGGCGGACA GCCTGATGAC CAGCAGCGCC TTCGACACCT CCCAGGTGAA GGACAAGCGG 
GACGCCATCA ACGGCCGTTT CCAGAAGATC AAGAGCATGG CCGCCTCGAG GCGAGCTAGG 
CTGAATGAGT CCCACCGCCT GCACCAGTTT TTCCGGGACA TGGATGATGA GGAGTCCTGG 
ATCAAGGAAA AGAAGCTGCT GGTGAGCTCA GAGGACTACG GCCGTGACCT GACGGGCGTC 
CAGAACCTGA GGAAGAAGCA CAAACGGCTG GAAGCAGAGC TGGCTGCCCA CGAGCCGGCC 
ATTCAGGGTG TCCTGGACAC TGGAAAGAAG CTGTCTGATG ACAACACCAT CGGGAAGGAG 
GAGATTCAGC AGCGCCTGGC GCAGTTCGTG GAGCACTGGA AGGAGCTGAA GCAGCTGGCA 
GCTGCCCGGG GCCAGCGGCT AGAAGAATCT TTGGAATATC AGCAGTTTGT AGCCAACGTG 
GAGGAGGAGG AAGCCTGGAT CAATGAGAAG ATGACCCTGG TGGCCAGTGA AGATTATGGA 
GACACTCTTG CTGCCATCCA GGGCTTGCTG AAGAAACACG AAGCCTTCGA GACGGACTTC 
ACTGTCCACA AGGACCGAGT GAATGATGTC TGCACCAACG GCCAGGACCT GGTTAAGAAG 
AACAATCACC ATGAGGAGAA CATCTCTTCC AAGATGAAGG GCTTGAACGG GAAAGTGTCA 
GACTTGGAGA AGGCTGCCTC CCAGAGGAAG GCCAAGCTCG ACGAGAACTC GGCCTTCCTC 
CAGTTCAACT GGAAAGCCGA TGTGGTGGAG TCCTGGATCG GTGAAAAGGA GAACAGCTTG 
AAGACAGATG ATTATGGTCG AGATCTGTCT TCTGTCCAAA CTCTCCTCAC CAAACAGGAA 
ACCTTCGATG CTGGCCTGCA GGCCTTCCAG CAGGAGGGCA TCGCCAACAT CACTGCCCTC 
AAAGATCAGC TGCTGGCCGC CAAGCACGTT CAGTCCAAGG CCATCGAGGC CCGCCATGCT 
GCCCTCATGA AGAGGTGGAG CCAGCTCCTG GCCAACTCGG CCACCCGGAA GAAGAAGCTG 
CTGGAGGCAC AGAGTCACTT CCGCAAGGTG GAGGATCTCT TCCTGACCTT TGCTAAAAAG 
GCTTCAGCCT TCAACAGCTG GTTTGAAAAC GCAGAGGAGG ACTTAACCGA CCCAGTGCGC 
TGCAACTCTC TGGAAGAGAT CAAGGCCTTG AGAGAGGCCC ACGACGCCTT CCGCTCCTCG 
TTGAGCTCGG CCCAGGCCGA CTTCAACCAG CTGGCAGAGC TGGACCGCCA GATCAAGAGC 
TTCCGCGTGG CCTCCAACCC CTACACCTGG TTCACCATGG AGGCCCTGGA GGAGACCTGG 
AGAAACCTAC AGAAGATCAT CAAGGAAAGG GAGCTGGAGC TGCAGAAGGA GCAGCGACGG 
CAGGAGGAGA ATGACAAGCT GCGCCAGGAG TTCGCCCAGC ATGCCAACGC CTTCCACCAG 
TGGATCCAGG AGACCAGAAC GTACCTCCTC GATGGCATAG CGTATCGTCG GGTCATTCGT 
GTCTATCAGT ATGAAGTTGG GGATGATCTG TCTGGAAGGT CCTGCATGGT GGAAGAATCC 
GGGACCCTCG AATCACAGCT TGAAGCTACC AAACGCAAAC ACCAGGAGAT TCGAGCTATG 
AGAAGTCAGC TCAAGAAGAT TGAAGACCTG GGGGCGGCCA TGGAGGAAGC GCTGATCCTG 
GACAACAAGT ACACGGAGCA CAGCACCGTG GGCCTGGCCC AGCAGTGGGA CCAGCTCGAC 
CAGTTGGGCA TGCGCATGCA GCACAACCTG GAGCAGCAGA TCCAGGCCAG GAACACGACC 
GGAGTGACCG AGGAGGCCCT CAAAGAATTC AGCATGATGT TCAAACACTT CGACAAGGAC 
AAGTCTGGCC GGCTGAACCA CCAGGAGTTC AAATCCTGTC TGCGCTCCCT GGGCTATGAC 
CTGCCCATGG TGGAGGAAGG GGAGCCCGAC CCTGAGTTCG AGGCCATCCT GGACACTGTG 
GATCCTAACA GGGACGGCCA CGTCTCCCTG CAAGAGTACA TGGCCTTTAT GATCAGCCGT 
GAGACCGAGA ACGTCAAGTC CAGCGAGGAG ATTGAGAGCG CCTTCCGGGC CCTGAGCTCT 
GAAGGGAAGC CTTATGTGAC CAAGGAGGAG CTGTACCAGA ACCTGACTCG GGAACAAGCC 
GACTACTGCG TCTCCCACAT GAAGCCCTAC GTGGACGGCA AGGGCCGCGA ACTCCCCACT 
GCTTTTGACT ACGTCGAGTT CACCCGCTCG CTCTTCGTGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_010810458.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010808760.2 Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa92003
Clone ID Related Accession (Same CDS sequence)	XM_010810458.2
Accession Version	XM_010810458.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7473bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810458.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGCGG GGTCAAAGTG 
CTGGAGACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTGGACAG ATACCACCGC 
TTCAAGGAGC TCTCGACCCT GAGGCGCCAG AAGCTTGAAG ACTCCTACCG ATTCCAGTTT 
TTCCAAAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAGCTTCA GATTGCCTCA 
GATGAGAATT ACAAAGACCC AACCAACCTG CAGGGCAAGC TGCAGAAGCA TCAAGCCTTC 
GAAGCCGAGG TGCAGGCCAA CTCGGGCGCC ATCGTGAAGC TGGATGAGAC TGGTAACCTG 
ATGATCTCAG AAGGGCACTT TGCATCCGAA ACCATCCGGA CTCGTCTGAT GGAGCTGCAC 
CGCCAGTGGG AATTGCTCCT GGAGAAGATG CGGGAGAAAG GCATCAAGCT GCTGCAGGCC 
CAGAAGCTGG TGCAGTACCT GCGGGAGTGT GAGGATGTGA TGGACTGGAT CAATGACAAG 
GAAGCCATTG TGACGTCTGA GGAGCTGGGC CAGGACCTGG AGCACGTGGA GGTGTTACAG 
AAGAAATTCG AAGAGTTTCA GACAGACATG GCTGCCCACG AAGAGAGAGT CAATGAAGTG 
AACCAGTTCG CTGCCAAGCT CATTCAGGAG CAGCACCCTG AGGAGGAGCT GATCAAGACA 
AAGCAGGACG AGGTGAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCCCT CCAGCGGCAG 
GGGAAGCTGT TCGGGGCAGC TGAGGTCCAA CGCTTTAACA GGGATGTGGA CGAGACCATC 
AGCTGGATTA AGGAGAAGGA ACAGTTAATG GCCTCTGATG ACTTTGGCCG GGACCTGGCC 
AGCGTCCAGG CTTTACTACG GAAACACGAG GGTTTGGAGA GAGATCTTGC TGCCTTGGAG 
GACAAGGTCA AAGCCCTGTG TGCCGAGGCT GACCGCCTGC ATCAGTCCCA CCCTCTGAGT 
GCCACCCAGA TCCAGGTGAA GCGAGAGGAG CTGATCACCA ACTGGGAGCA GATCCGCACC 
CTGGCCGCGG AGAGACACGC ACGTCTCAAC GATTCCTACA GGCTTCAGCG CTTCCTTGCC 
GACTTCCGAG ACCTCACCAG CTGGGTCACT GAGATGAAAG CCCTCATCAA CGCAGATGAA 
CTTGCCAACG ATGTGGCCGG AGCCGAAGCC CTGCTGGATA GGCATCAGGA GCACAAGGGT 
GAAATTGACG CCCATGAAGA TAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT 
GCAGCTGGTC ACTACGCCTC AGATGAAGTG AGGGAGAAGC TTACCGTGCT GTCCGAGGAG 
CGGGCCGCGC TGCTGGAGCT CTGGGAGCTG CGACGGCAGC AATATGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ACGAAGACCT GGGCGATTCC TTGGATAGTG TGGAAGCGCT TCTAAAGAAG 
CACGAAGACT TTGAGAAGTC CCTCAGCGCC CAGGAGGAGA AGATCACAGC CTTAGATGAG 
TTTGCCACCA AGCTGATTCA GAACAACCAC TACGCAATGG AAGATGTGGC CACCCGGCGA 
GACGCACTGC TGAGCCGTCG CAATGCCCTG CATGAGAGAG CCATGTACCG CCGTGCCCAG 
CTTGCAGATT CCTTCCACTT GCAGCAGTTT TTCCGTGACT CTGATGAGCT CAAGAGTTGG 
GTCAACGAGA AGATGAAAAC AGCCACGGAT GAAGCTTATA AAGACCCATC CAACCTACAA 
GGGAAAGTGC AGAAGCACCA GGCCTTTGAG GCCGAGCTCT CGGCAAACCA GAGCCGCATC 
GACGCCCTGG AGAAAGCCGG GCAGAAGCTG ATCGATGTCG ACCACTATGC CAAGGACGAG 
GTGGCGGCTC GGATGAATGA GGTCATCAGT CTGTGGAAGA AGCTGCTGGA GGCCACAGAG 
CTGAAAGGCA TAAAGCTCCG CGAGGCTAAC CAGCAGCAGC AGTTTAATCG CAACGTTGAG 
GACATCGAGC TGTGGCTGTA TGAAGTGGAA GGTCATCTGG CCTCGGACGA CTACGGCAAA 
GACCTCACCA ACGTCCAGAA CCTCCAGAAG AAGCACGCCC TGCTGGAGGC TGACGTGGCA 
GCTCACCAGG ATCGCATCGA TGGCATCACT ATCCAAGCCC GCCAGTTCCA GGACGCGGGC 
CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAGGCCC TGGTAGCTCG CTACGAGGCG 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCTG ATTCCCTGCG GTTGCAGCAG 
CTCTTCCGTG ACGTCGAGGA TGAGGAGACG TGGATTCGGG AGAAAGAACC CATTGCCGCC 
TCCACCAACA GAGGCAAAGA TTTAATTGGA GTCCAGAACC TGCTAAAGAA ACACCAAGCG 
CTACAAGCAG AAATTGCTGG CCACGAGCCC CGCATCAAAG CAGTCACACA GAAGGGGAAC 
GCCATGGTGG AGGAGGGCCA TTTTGCTGCT GAGGACGTGA AGGCCAAGCT CCATGAGCTG 
AACCAGAAGT GGGAGTCGCT GAAGTCCAAG GCCTCCCAGC GGCGGCAGGA CCTGGAGGAC 
TCGCTGCAGG CCCAGCAGTA CTTTGCCGAC GCCAACGAGG CTGAGTCCTG GATGCGGGAG 
AAGGAGCCCA TCGTGGGCAG CACCGACTAT GGCAAAGATG AGGACTCGGC CGAGGCTCTG 
CTAAAGAAGC ACGAGGCATT GATGTCGGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCC 
CTGCGAGAGC AGGCCCAGTC GTGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG 
AAGGAGCTGG TCTTGGCGCT CTATGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAAGGAG ACATTCTCAC CTTACTCAAC AGCACCAACA AGGACTGGTG GAAAGTGGAA 
GTGAATGACC GGCAGGGCTT CGTGCCGGCT GCCTACGTGA AGAAGCTGGA TCCTGCGCAG 
TCGGCCTCCC GGGAGAACCT CCTGGAAGAG CAGGGCAGCA TCGCGCTGCG GCAGGAACAG 
ATCGACAACC AGTACCATTC TCTGCTGGAA CTGGGAGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGCTGTA AGAAGTTCAT GTTGTTCCGT GAAGCGAACG AGCTACAGCA GTGGATCAAT 
GAGAAAGAAG CGGCCCTGAC AAGTGAGGAG GTTGGCGCGG ACTTGGAGCA GGTTGAAGTG 
CTGCAGAAGA AGTTTGACGA CTTCCAGAAG GATCTCAAGG CCAATGAGTC GCGGCTGAAG 
GACATTAACA AGGTGGCTGA AGACCTGGAG TCGGAAGGTC TGATGGCGGA GGAGGTGCAG 
GCCGTGCAGC AGCAGGAGGT GTATGGCGCG ATGCCCAGGG ATGAATCTGA TTCCAAGACA 
GCCTCCCCTT GGAAGTCTGC TCGCCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTGA ACGAGCGCTG GCGGTCCCTG CAGCAGCTGG CTGAGGAGCG GAGCCAACTC 
TTGGGCAGTG CCCATGAAGT GCAGCGGTTC CACAGAGATG CTGACGAAAC CAAAGAGTGG 
ATCGAAGAGA AGAACCAGGC TCTAAACACA GACAACTACG GCCACGACCT GGCCAGTGTC 
CAGGCCCTGC AGCGCAAGCA CGAGGGCTTC GAGAGGGACC TCGCAGCCCT TGGTGACAAG 
GTGAACTCGC TCGGGGAGAC AGCCGAGCGC CTGATCCAGT CCCACCCCGA GTCGGCAGAG 
GACCTGCAGG AGAAGTGCAC TGAGCTGAAC CAGGCATGGA GCAGCCTGGG CAAGCGTGCC 
GACCAGCGCA AGGCCAAGCT GGGCGACTCC CACGACCTGC AGCGCTTCCT CAGCGACTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATC CGAGGTCTGG TGTCCTCAGA CGAGCTAGCC 
AAGGATGTCA CCGGAGCTGA GGCCCTGCTG GAGCGACACC AGGAACACCG GACAGAAATT 
GATGCCAGAG CTGGCACTTT CCAGGCATTC GAGCAGTTTG GGCAGCAGCT GTTGGCTCAT 
GGGCACTACG CCAGCCCCGA GATCAAGGAG AAGCTCGATG TTCTAGACCA GGAGCGTGCA 
GACCTTGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAATTGCAG 
CTGTTCCATC GGGATTGCGA GCAGGCCGAG AACTGGATGG CTGCCCGAGA GGCCTTTCTG 
AACACAGAAG ACAAAGGGGA CTCGCTAGAC AGTGTGGAGG CTCTGATCAA AAAACACGAG 
GACTTTGATA AAGCAATTAA TGTCCAGGAG GAGAAGATTG CTGCCCTGCA GTCCTTTGCT 
GACCAGCTCA TCGCTGGTGG CCACTACGCC AAAGGCGACA TCTCCAGCCG GCGCAATGAG 
GTTCTGGACA GGTGGCGCCG TCTAAAAGCT CAGATGATTG AGAAGAGGTC AAAGCTGGGA 
GAATCACAGA CCCTCCAGCA GTTCAGCCGG GATGTAGACG AGATTGAAGC TTGGATCAGT 
GAAAAATTAC AGACTGCAAG TGATGAGTCA TATAAGGACC CCACCAACAT CCAGCTTTCC 
AAGCTGCTGA GCAAGCATCA GAAGCACCAG GCCTTCGAGG CAGAACTGCA CGCCAATGCG 
GACCGGATCC GTGGGGTCAT CGACATGGGC AACTCCCTCA TTGACCGCGG GGCCTGCGCC 
GGCAGCGAGG ATGCCGTCAA GGCCCGGCTG GCCGCCTTAG CTGACCAGTG GCAGTTCCTG 
GTGCAGAAGT CGGCTGAGAA GAGCCAGAAG CTGAAAGAAG CCAACAAGCA GCAGAACTTC 
AACACTGGCA TCAAGGACTT CGACTTCTGG CTCTCTGAGG TGGAAGCCCT GCTGGCCTCT 
GAGGATTATG GCAAAGACCT GGCTTCTGTG AACAACTTGC TAAAAAAACA TCAGCTGCTG 
GAGGCAGATA TCTCTGCCCA CGAGGACCGC CTGAAGGACC TCAACAGCCA GGCGGACAGC 
CTGATGACCA GCAGCGCCTT CGACACCTCC CAGGTGAAGG ACAAGCGGGA CGCCATCAAC 
GGCCGTTTCC AGAAGATCAA GAGCATGGCC GCCTCGAGGC GAGCTAGGCT GAATGAGTCC 
CACCGCCTGC ACCAGTTTTT CCGGGACATG GATGATGAGG AGTCCTGGAT CAAGGAAAAG 
AAGCTGCTGG TGAGCTCAGA GGACTACGGC CGTGACCTGA CGGGCGTCCA GAACCTGAGG 
AAGAAGCACA AACGGCTGGA AGCAGAGCTG GCTGCCCACG AGCCGGCCAT TCAGGGTGTC 
CTGGACACTG GAAAGAAGCT GTCTGATGAC AACACCATCG GGAAGGAGGA GATTCAGCAG 
CGCCTGGCGC AGTTCGTGGA GCACTGGAAG GAGCTGAAGC AGCTGGCAGC TGCCCGGGGC 
CAGCGGCTAG AAGAATCTTT GGAATATCAG CAGTTTGTAG CCAACGTGGA GGAGGAGGAA 
GCCTGGATCA ATGAGAAGAT GACCCTGGTG GCCAGTGAAG ATTATGGAGA CACTCTTGCT 
GCCATCCAGG GCTTGCTGAA GAAACACGAA GCCTTCGAGA CGGACTTCAC TGTCCACAAG 
GACCGAGTGA ATGATGTCTG CACCAACGGC CAGGACCTGG TTAAGAAGAA CAATCACCAT 
GAGGAGAACA TCTCTTCCAA GATGAAGGGC TTGAACGGGA AAGTGTCAGA CTTGGAGAAG 
GCTGCCTCCC AGAGGAAGGC CAAGCTCGAC GAGAACTCGG CCTTCCTCCA GTTCAACTGG 
AAAGCCGATG TGGTGGAGTC CTGGATCGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT 
TATGGTCGAG ATCTGTCTTC TGTCCAAACT CTCCTCACCA AACAGGAAAC CTTCGATGCT 
GGCCTGCAGG CCTTCCAGCA GGAGGGCATC GCCAACATCA CTGCCCTCAA AGATCAGCTG 
CTGGCCGCCA AGCACGTTCA GTCCAAGGCC ATCGAGGCCC GCCATGCTGC CCTCATGAAG 
AGGTGGAGCC AGCTCCTGGC CAACTCGGCC ACCCGGAAGA AGAAGCTGCT GGAGGCACAG 
AGTCACTTCC GCAAGGTGGA GGATCTCTTC CTGACCTTTG CTAAAAAGGC TTCAGCCTTC 
AACAGCTGGT TTGAAAACGC AGAGGAGGAC TTAACCGACC CAGTGCGCTG CAACTCTCTG 
GAAGAGATCA AGGCCTTGAG AGAGGCCCAC GACGCCTTCC GCTCCTCGTT GAGCTCGGCC 
CAGGCCGACT TCAACCAGCT GGCAGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTGGCC 
TCCAACCCCT ACACCTGGTT CACCATGGAG GCCCTGGAGG AGACCTGGAG AAACCTACAG 
AAGATCATCA AGGAAAGGGA GCTGGAGCTG CAGAAGGAGC AGCGACGGCA GGAGGAGAAT 
GACAAGCTGC GCCAGGAGTT CGCCCAGCAT GCCAACGCCT TCCACCAGTG GATCCAGGAG 
ACCAGAACGT ACCTCCTCGA TGGCATAGCG TATCGTCGGG TCATTCGTGT CTATCAGTAT 
GAAGTTGGGG ATGATCTGTC TGGAAGGTCC TGCATGGTGG AAGAATCCGG GACCCTCGAA 
TCACAGCTTG AAGCTACCAA ACGCAAACAC CAGGAGATTC GAGCTATGAG AAGTCAGCTC 
AAGAAGATTG AAGACCTGGG GGCGGCCATG GAGGAAGCGC TGATCCTGGA CAACAAGTAC 
ACGGAGCACA GCACCGTGGG CCTGGCCCAG CAGTGGGACC AGCTCGACCA GTTGGGCATG 
CGCATGCAGC ACAACCTGGA GCAGCAGATC CAGGCCAGGA ACACGACCGG AGTGACCGAG 
GAGGCCCTCA AAGAATTCAG CATGATGTTC AAACACTTCG ACAAGGACAA GTCTGGCCGG 
CTGAACCACC AGGAGTTCAA ATCCTGTCTG CGCTCCCTGG GCTATGACCT GCCCATGGTG 
GAGGAAGGGG AGCCCGACCC TGAGTTCGAG GCCATCCTGG ACACTGTGGA TCCTAACAGG 
GACGGCCACG TCTCCCTGCA AGAGTACATG GCCTTTATGA TCAGCCGTGA GACCGAGAAC 
GTCAAGTCCA GCGAGGAGAT TGAGAGCGCC TTCCGGGCCC TGAGCTCTGA AGGGAAGCCT 
TATGTGACCA AGGAGGAGCT GTACCAGAAC CTGACTCGGG AACAAGCCGA CTACTGCGTC 
TCCCACATGA AGCCCTACGT GGACGGCAAG GGCCGCGAAC TCCCCACTGC TTTTGACTAC 
GTCGAGTTCA CCCGCTCGCT CTTCGTGAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010808760.2
CDS	55..7527
Translation	MLSSFRKRRVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLHQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMYRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVDHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWESLKSKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGAMPRDESDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKEKLDVLDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQSFADQLIAGGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDMGNSLIDRGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDAINGRFQKIKSMAASRRARLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLVKKNNHHEENISSKMKGLNGKVSDLEKAASQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHAALMKRWSQLLANSATRKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGIAYRRVIRVYQYEVGDDLSGRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_010810458.2
Organism	Bos taurus(cattle)
Definition	Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010810458.2

ORF Insert Sequence:

ATGCTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGCGG GGTCAAAGTG 
CTGGAGACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTGGACAG ATACCACCGC 
TTCAAGGAGC TCTCGACCCT GAGGCGCCAG AAGCTTGAAG ACTCCTACCG ATTCCAGTTT 
TTCCAAAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAGCTTCA GATTGCCTCA 
GATGAGAATT ACAAAGACCC AACCAACCTG CAGGGCAAGC TGCAGAAGCA TCAAGCCTTC 
GAAGCCGAGG TGCAGGCCAA CTCGGGCGCC ATCGTGAAGC TGGATGAGAC TGGTAACCTG 
ATGATCTCAG AAGGGCACTT TGCATCCGAA ACCATCCGGA CTCGTCTGAT GGAGCTGCAC 
CGCCAGTGGG AATTGCTCCT GGAGAAGATG CGGGAGAAAG GCATCAAGCT GCTGCAGGCC 
CAGAAGCTGG TGCAGTACCT GCGGGAGTGT GAGGATGTGA TGGACTGGAT CAATGACAAG 
GAAGCCATTG TGACGTCTGA GGAGCTGGGC CAGGACCTGG AGCACGTGGA GGTGTTACAG 
AAGAAATTCG AAGAGTTTCA GACAGACATG GCTGCCCACG AAGAGAGAGT CAATGAAGTG 
AACCAGTTCG CTGCCAAGCT CATTCAGGAG CAGCACCCTG AGGAGGAGCT GATCAAGACA 
AAGCAGGACG AGGTGAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCCCT CCAGCGGCAG 
GGGAAGCTGT TCGGGGCAGC TGAGGTCCAA CGCTTTAACA GGGATGTGGA CGAGACCATC 
AGCTGGATTA AGGAGAAGGA ACAGTTAATG GCCTCTGATG ACTTTGGCCG GGACCTGGCC 
AGCGTCCAGG CTTTACTACG GAAACACGAG GGTTTGGAGA GAGATCTTGC TGCCTTGGAG 
GACAAGGTCA AAGCCCTGTG TGCCGAGGCT GACCGCCTGC ATCAGTCCCA CCCTCTGAGT 
GCCACCCAGA TCCAGGTGAA GCGAGAGGAG CTGATCACCA ACTGGGAGCA GATCCGCACC 
CTGGCCGCGG AGAGACACGC ACGTCTCAAC GATTCCTACA GGCTTCAGCG CTTCCTTGCC 
GACTTCCGAG ACCTCACCAG CTGGGTCACT GAGATGAAAG CCCTCATCAA CGCAGATGAA 
CTTGCCAACG ATGTGGCCGG AGCCGAAGCC CTGCTGGATA GGCATCAGGA GCACAAGGGT 
GAAATTGACG CCCATGAAGA TAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT 
GCAGCTGGTC ACTACGCCTC AGATGAAGTG AGGGAGAAGC TTACCGTGCT GTCCGAGGAG 
CGGGCCGCGC TGCTGGAGCT CTGGGAGCTG CGACGGCAGC AATATGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ACGAAGACCT GGGCGATTCC TTGGATAGTG TGGAAGCGCT TCTAAAGAAG 
CACGAAGACT TTGAGAAGTC CCTCAGCGCC CAGGAGGAGA AGATCACAGC CTTAGATGAG 
TTTGCCACCA AGCTGATTCA GAACAACCAC TACGCAATGG AAGATGTGGC CACCCGGCGA 
GACGCACTGC TGAGCCGTCG CAATGCCCTG CATGAGAGAG CCATGTACCG CCGTGCCCAG 
CTTGCAGATT CCTTCCACTT GCAGCAGTTT TTCCGTGACT CTGATGAGCT CAAGAGTTGG 
GTCAACGAGA AGATGAAAAC AGCCACGGAT GAAGCTTATA AAGACCCATC CAACCTACAA 
GGGAAAGTGC AGAAGCACCA GGCCTTTGAG GCCGAGCTCT CGGCAAACCA GAGCCGCATC 
GACGCCCTGG AGAAAGCCGG GCAGAAGCTG ATCGATGTCG ACCACTATGC CAAGGACGAG 
GTGGCGGCTC GGATGAATGA GGTCATCAGT CTGTGGAAGA AGCTGCTGGA GGCCACAGAG 
CTGAAAGGCA TAAAGCTCCG CGAGGCTAAC CAGCAGCAGC AGTTTAATCG CAACGTTGAG 
GACATCGAGC TGTGGCTGTA TGAAGTGGAA GGTCATCTGG CCTCGGACGA CTACGGCAAA 
GACCTCACCA ACGTCCAGAA CCTCCAGAAG AAGCACGCCC TGCTGGAGGC TGACGTGGCA 
GCTCACCAGG ATCGCATCGA TGGCATCACT ATCCAAGCCC GCCAGTTCCA GGACGCGGGC 
CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAGGCCC TGGTAGCTCG CTACGAGGCG 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCTG ATTCCCTGCG GTTGCAGCAG 
CTCTTCCGTG ACGTCGAGGA TGAGGAGACG TGGATTCGGG AGAAAGAACC CATTGCCGCC 
TCCACCAACA GAGGCAAAGA TTTAATTGGA GTCCAGAACC TGCTAAAGAA ACACCAAGCG 
CTACAAGCAG AAATTGCTGG CCACGAGCCC CGCATCAAAG CAGTCACACA GAAGGGGAAC 
GCCATGGTGG AGGAGGGCCA TTTTGCTGCT GAGGACGTGA AGGCCAAGCT CCATGAGCTG 
AACCAGAAGT GGGAGTCGCT GAAGTCCAAG GCCTCCCAGC GGCGGCAGGA CCTGGAGGAC 
TCGCTGCAGG CCCAGCAGTA CTTTGCCGAC GCCAACGAGG CTGAGTCCTG GATGCGGGAG 
AAGGAGCCCA TCGTGGGCAG CACCGACTAT GGCAAAGATG AGGACTCGGC CGAGGCTCTG 
CTAAAGAAGC ACGAGGCATT GATGTCGGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCC 
CTGCGAGAGC AGGCCCAGTC GTGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG 
AAGGAGCTGG TCTTGGCGCT CTATGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAAGGAG ACATTCTCAC CTTACTCAAC AGCACCAACA AGGACTGGTG GAAAGTGGAA 
GTGAATGACC GGCAGGGCTT CGTGCCGGCT GCCTACGTGA AGAAGCTGGA TCCTGCGCAG 
TCGGCCTCCC GGGAGAACCT CCTGGAAGAG CAGGGCAGCA TCGCGCTGCG GCAGGAACAG 
ATCGACAACC AGTACCATTC TCTGCTGGAA CTGGGAGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGCTGTA AGAAGTTCAT GTTGTTCCGT GAAGCGAACG AGCTACAGCA GTGGATCAAT 
GAGAAAGAAG CGGCCCTGAC AAGTGAGGAG GTTGGCGCGG ACTTGGAGCA GGTTGAAGTG 
CTGCAGAAGA AGTTTGACGA CTTCCAGAAG GATCTCAAGG CCAATGAGTC GCGGCTGAAG 
GACATTAACA AGGTGGCTGA AGACCTGGAG TCGGAAGGTC TGATGGCGGA GGAGGTGCAG 
GCCGTGCAGC AGCAGGAGGT GTATGGCGCG ATGCCCAGGG ATGAATCTGA TTCCAAGACA 
GCCTCCCCTT GGAAGTCTGC TCGCCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTGA ACGAGCGCTG GCGGTCCCTG CAGCAGCTGG CTGAGGAGCG GAGCCAACTC 
TTGGGCAGTG CCCATGAAGT GCAGCGGTTC CACAGAGATG CTGACGAAAC CAAAGAGTGG 
ATCGAAGAGA AGAACCAGGC TCTAAACACA GACAACTACG GCCACGACCT GGCCAGTGTC 
CAGGCCCTGC AGCGCAAGCA CGAGGGCTTC GAGAGGGACC TCGCAGCCCT TGGTGACAAG 
GTGAACTCGC TCGGGGAGAC AGCCGAGCGC CTGATCCAGT CCCACCCCGA GTCGGCAGAG 
GACCTGCAGG AGAAGTGCAC TGAGCTGAAC CAGGCATGGA GCAGCCTGGG CAAGCGTGCC 
GACCAGCGCA AGGCCAAGCT GGGCGACTCC CACGACCTGC AGCGCTTCCT CAGCGACTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATC CGAGGTCTGG TGTCCTCAGA CGAGCTAGCC 
AAGGATGTCA CCGGAGCTGA GGCCCTGCTG GAGCGACACC AGGAACACCG GACAGAAATT 
GATGCCAGAG CTGGCACTTT CCAGGCATTC GAGCAGTTTG GGCAGCAGCT GTTGGCTCAT 
GGGCACTACG CCAGCCCCGA GATCAAGGAG AAGCTCGATG TTCTAGACCA GGAGCGTGCA 
GACCTTGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAATTGCAG 
CTGTTCCATC GGGATTGCGA GCAGGCCGAG AACTGGATGG CTGCCCGAGA GGCCTTTCTG 
AACACAGAAG ACAAAGGGGA CTCGCTAGAC AGTGTGGAGG CTCTGATCAA AAAACACGAG 
GACTTTGATA AAGCAATTAA TGTCCAGGAG GAGAAGATTG CTGCCCTGCA GTCCTTTGCT 
GACCAGCTCA TCGCTGGTGG CCACTACGCC AAAGGCGACA TCTCCAGCCG GCGCAATGAG 
GTTCTGGACA GGTGGCGCCG TCTAAAAGCT CAGATGATTG AGAAGAGGTC AAAGCTGGGA 
GAATCACAGA CCCTCCAGCA GTTCAGCCGG GATGTAGACG AGATTGAAGC TTGGATCAGT 
GAAAAATTAC AGACTGCAAG TGATGAGTCA TATAAGGACC CCACCAACAT CCAGCTTTCC 
AAGCTGCTGA GCAAGCATCA GAAGCACCAG GCCTTCGAGG CAGAACTGCA CGCCAATGCG 
GACCGGATCC GTGGGGTCAT CGACATGGGC AACTCCCTCA TTGACCGCGG GGCCTGCGCC 
GGCAGCGAGG ATGCCGTCAA GGCCCGGCTG GCCGCCTTAG CTGACCAGTG GCAGTTCCTG 
GTGCAGAAGT CGGCTGAGAA GAGCCAGAAG CTGAAAGAAG CCAACAAGCA GCAGAACTTC 
AACACTGGCA TCAAGGACTT CGACTTCTGG CTCTCTGAGG TGGAAGCCCT GCTGGCCTCT 
GAGGATTATG GCAAAGACCT GGCTTCTGTG AACAACTTGC TAAAAAAACA TCAGCTGCTG 
GAGGCAGATA TCTCTGCCCA CGAGGACCGC CTGAAGGACC TCAACAGCCA GGCGGACAGC 
CTGATGACCA GCAGCGCCTT CGACACCTCC CAGGTGAAGG ACAAGCGGGA CGCCATCAAC 
GGCCGTTTCC AGAAGATCAA GAGCATGGCC GCCTCGAGGC GAGCTAGGCT GAATGAGTCC 
CACCGCCTGC ACCAGTTTTT CCGGGACATG GATGATGAGG AGTCCTGGAT CAAGGAAAAG 
AAGCTGCTGG TGAGCTCAGA GGACTACGGC CGTGACCTGA CGGGCGTCCA GAACCTGAGG 
AAGAAGCACA AACGGCTGGA AGCAGAGCTG GCTGCCCACG AGCCGGCCAT TCAGGGTGTC 
CTGGACACTG GAAAGAAGCT GTCTGATGAC AACACCATCG GGAAGGAGGA GATTCAGCAG 
CGCCTGGCGC AGTTCGTGGA GCACTGGAAG GAGCTGAAGC AGCTGGCAGC TGCCCGGGGC 
CAGCGGCTAG AAGAATCTTT GGAATATCAG CAGTTTGTAG CCAACGTGGA GGAGGAGGAA 
GCCTGGATCA ATGAGAAGAT GACCCTGGTG GCCAGTGAAG ATTATGGAGA CACTCTTGCT 
GCCATCCAGG GCTTGCTGAA GAAACACGAA GCCTTCGAGA CGGACTTCAC TGTCCACAAG 
GACCGAGTGA ATGATGTCTG CACCAACGGC CAGGACCTGG TTAAGAAGAA CAATCACCAT 
GAGGAGAACA TCTCTTCCAA GATGAAGGGC TTGAACGGGA AAGTGTCAGA CTTGGAGAAG 
GCTGCCTCCC AGAGGAAGGC CAAGCTCGAC GAGAACTCGG CCTTCCTCCA GTTCAACTGG 
AAAGCCGATG TGGTGGAGTC CTGGATCGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT 
TATGGTCGAG ATCTGTCTTC TGTCCAAACT CTCCTCACCA AACAGGAAAC CTTCGATGCT 
GGCCTGCAGG CCTTCCAGCA GGAGGGCATC GCCAACATCA CTGCCCTCAA AGATCAGCTG 
CTGGCCGCCA AGCACGTTCA GTCCAAGGCC ATCGAGGCCC GCCATGCTGC CCTCATGAAG 
AGGTGGAGCC AGCTCCTGGC CAACTCGGCC ACCCGGAAGA AGAAGCTGCT GGAGGCACAG 
AGTCACTTCC GCAAGGTGGA GGATCTCTTC CTGACCTTTG CTAAAAAGGC TTCAGCCTTC 
AACAGCTGGT TTGAAAACGC AGAGGAGGAC TTAACCGACC CAGTGCGCTG CAACTCTCTG 
GAAGAGATCA AGGCCTTGAG AGAGGCCCAC GACGCCTTCC GCTCCTCGTT GAGCTCGGCC 
CAGGCCGACT TCAACCAGCT GGCAGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTGGCC 
TCCAACCCCT ACACCTGGTT CACCATGGAG GCCCTGGAGG AGACCTGGAG AAACCTACAG 
AAGATCATCA AGGAAAGGGA GCTGGAGCTG CAGAAGGAGC AGCGACGGCA GGAGGAGAAT 
GACAAGCTGC GCCAGGAGTT CGCCCAGCAT GCCAACGCCT TCCACCAGTG GATCCAGGAG 
ACCAGAACGT ACCTCCTCGA TGGCATAGCG TATCGTCGGG TCATTCGTGT CTATCAGTAT 
GAAGTTGGGG ATGATCTGTC TGGAAGGTCC TGCATGGTGG AAGAATCCGG GACCCTCGAA 
TCACAGCTTG AAGCTACCAA ACGCAAACAC CAGGAGATTC GAGCTATGAG AAGTCAGCTC 
AAGAAGATTG AAGACCTGGG GGCGGCCATG GAGGAAGCGC TGATCCTGGA CAACAAGTAC 
ACGGAGCACA GCACCGTGGG CCTGGCCCAG CAGTGGGACC AGCTCGACCA GTTGGGCATG 
CGCATGCAGC ACAACCTGGA GCAGCAGATC CAGGCCAGGA ACACGACCGG AGTGACCGAG 
GAGGCCCTCA AAGAATTCAG CATGATGTTC AAACACTTCG ACAAGGACAA GTCTGGCCGG 
CTGAACCACC AGGAGTTCAA ATCCTGTCTG CGCTCCCTGG GCTATGACCT GCCCATGGTG 
GAGGAAGGGG AGCCCGACCC TGAGTTCGAG GCCATCCTGG ACACTGTGGA TCCTAACAGG 
GACGGCCACG TCTCCCTGCA AGAGTACATG GCCTTTATGA TCAGCCGTGA GACCGAGAAC 
GTCAAGTCCA GCGAGGAGAT TGAGAGCGCC TTCCGGGCCC TGAGCTCTGA AGGGAAGCCT 
TATGTGACCA AGGAGGAGCT GTACCAGAAC CTGACTCGGG AACAAGCCGA CTACTGCGTC 
TCCCACATGA AGCCCTACGT GGACGGCAAG GGCCGCGAAC TCCCCACTGC TTTTGACTAC 
GTCGAGTTCA CCCGCTCGCT CTTCGTGAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_010810460.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010808762.2 Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBa92004
Clone ID Related Accession (Same CDS sequence)	XM_010810460.2
Accession Version	XM_010810460.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7452bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810460.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGCGG GGTCAAAGTG 
CTGGAGACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTGGACAG ATACCACCGC 
TTCAAGGAGC TCTCGACCCT GAGGCGCCAG AAGCTTGAAG ACTCCTACCG ATTCCAGTTT 
TTCCAAAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAGCTTCA GATTGCCTCA 
GATGAGAATT ACAAAGACCC AACCAACCTG CAGGGCAAGC TGCAGAAGCA TCAAGCCTTC 
GAAGCCGAGG TGCAGGCCAA CTCGGGCGCC ATCGTGAAGC TGGATGAGAC TGGTAACCTG 
ATGATCTCAG AAGGGCACTT TGCATCCGAA ACCATCCGGA CTCGTCTGAT GGAGCTGCAC 
CGCCAGTGGG AATTGCTCCT GGAGAAGATG CGGGAGAAAG GCATCAAGCT GCTGCAGGCC 
CAGAAGCTGG TGCAGTACCT GCGGGAGTGT GAGGATGTGA TGGACTGGAT CAATGACAAG 
GAAGCCATTG TGACGTCTGA GGAGCTGGGC CAGGACCTGG AGCACGTGGA GGTGTTACAG 
AAGAAATTCG AAGAGTTTCA GACAGACATG GCTGCCCACG AAGAGAGAGT CAATGAAGTG 
AACCAGTTCG CTGCCAAGCT CATTCAGGAG CAGCACCCTG AGGAGGAGCT GATCAAGACA 
AAGCAGGACG AGGTGAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCCCT CCAGCGGCAG 
GGGAAGCTGT TCGGGGCAGC TGAGGTCCAA CGCTTTAACA GGGATGTGGA CGAGACCATC 
AGCTGGATTA AGGAGAAGGA ACAGTTAATG GCCTCTGATG ACTTTGGCCG GGACCTGGCC 
AGCGTCCAGG CTTTACTACG GAAACACGAG GGTTTGGAGA GAGATCTTGC TGCCTTGGAG 
GACAAGGTCA AAGCCCTGTG TGCCGAGGCT GACCGCCTGC ATCAGTCCCA CCCTCTGAGT 
GCCACCCAGA TCCAGGTGAA GCGAGAGGAG CTGATCACCA ACTGGGAGCA GATCCGCACC 
CTGGCCGCGG AGAGACACGC ACGTCTCAAC GATTCCTACA GGCTTCAGCG CTTCCTTGCC 
GACTTCCGAG ACCTCACCAG CTGGGTCACT GAGATGAAAG CCCTCATCAA CGCAGATGAA 
CTTGCCAACG ATGTGGCCGG AGCCGAAGCC CTGCTGGATA GGCATCAGGA GCACAAGGGT 
GAAATTGACG CCCATGAAGA TAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT 
GCAGCTGGTC ACTACGCCTC AGATGAAGTG AGGGAGAAGC TTACCGTGCT GTCCGAGGAG 
CGGGCCGCGC TGCTGGAGCT CTGGGAGCTG CGACGGCAGC AATATGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ACGAAGACCT GGGCGATTCC TTGGATAGTG TGGAAGCGCT TCTAAAGAAG 
CACGAAGACT TTGAGAAGTC CCTCAGCGCC CAGGAGGAGA AGATCACAGC CTTAGATGAG 
TTTGCCACCA AGCTGATTCA GAACAACCAC TACGCAATGG AAGATGTGGC CACCCGGCGA 
GACGCACTGC TGAGCCGTCG CAATGCCCTG CATGAGAGAG CCATGTACCG CCGTGCCCAG 
CTTGCAGATT CCTTCCACTT GCAGCAGTTT TTCCGTGACT CTGATGAGCT CAAGAGTTGG 
GTCAACGAGA AGATGAAAAC AGCCACGGAT GAAGCTTATA AAGACCCATC CAACCTACAA 
GGGAAAGTGC AGAAGCACCA GGCCTTTGAG GCCGAGCTCT CGGCAAACCA GAGCCGCATC 
GACGCCCTGG AGAAAGCCGG GCAGAAGCTG ATCGATGTCG ACCACTATGC CAAGGACGAG 
GTGGCGGCTC GGATGAATGA GGTCATCAGT CTGTGGAAGA AGCTGCTGGA GGCCACAGAG 
CTGAAAGGCA TAAAGCTCCG CGAGGCTAAC CAGCAGCAGC AGTTTAATCG CAACGTTGAG 
GACATCGAGC TGTGGCTGTA TGAAGTGGAA GGTCATCTGG CCTCGGACGA CTACGGCAAA 
GACCTCACCA ACGTCCAGAA CCTCCAGAAG AAGCACGCCC TGCTGGAGGC TGACGTGGCA 
GCTCACCAGG ATCGCATCGA TGGCATCACT ATCCAAGCCC GCCAGTTCCA GGACGCGGGC 
CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAGGCCC TGGTAGCTCG CTACGAGGCG 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCTG ATTCCCTGCG GTTGCAGCAG 
CTCTTCCGTG ACGTCGAGGA TGAGGAGACG TGGATTCGGG AGAAAGAACC CATTGCCGCC 
TCCACCAACA GAGGCAAAGA TTTAATTGGA GTCCAGAACC TGCTAAAGAA ACACCAAGCG 
CTACAAGCAG AAATTGCTGG CCACGAGCCC CGCATCAAAG CAGTCACACA GAAGGGGAAC 
GCCATGGTGG AGGAGGGCCA TTTTGCTGCT GAGGACGTGA AGGCCAAGCT CCATGAGCTG 
AACCAGAAGT GGGAGTCGCT GAAGTCCAAG GCCTCCCAGC GGCGGCAGGA CCTGGAGGAC 
TCGCTGCAGG CCCAGCAGTA CTTTGCCGAC GCCAACGAGG CTGAGTCCTG GATGCGGGAG 
AAGGAGCCCA TCGTGGGCAG CACCGACTAT GGCAAAGATG AGGACTCGGC CGAGGCTCTG 
CTAAAGAAGC ACGAGGCATT GATGTCGGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCC 
CTGCGAGAGC AGGCCCAGTC GTGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG 
AAGGAGCTGG TCTTGGCGCT CTATGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAAGGAG ACATTCTCAC CTTACTCAAC AGCACCAACA AGGACTGGTG GAAAGTGGAA 
GTGAATGACC GGCAGGGCTT CGTGCCGGCT GCCTACGTGA AGAAGCTGGA TCCTGCGCAG 
TCGGCCTCCC GGGAGAACCT CCTGGAAGAG CAGGGCAGCA TCGCGCTGCG GCAGGAACAG 
ATCGACAACC AGACGCGCGT AACTAAGGAG GCTGGCAGCG TGTCTCTGCG TATGAAACAG 
GTGGAAGAAC TGTACCATTC TCTGCTGGAA CTGGGAGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGCTGTA AGAAGTTCAT GTTGTTCCGT GAAGCGAACG AGCTACAGCA GTGGATCAAT 
GAGAAAGAAG CGGCCCTGAC AAGTGAGGAG GTTGGCGCGG ACTTGGAGCA GGTTGAAGTG 
CTGCAGAAGA AGTTTGACGA CTTCCAGAAG GATCTCAAGG CCAATGAGTC GCGGCTGAAG 
GACATTAACA AGGTGGCTGA AGACCTGGAG TCGGAAGGTC TGATGGCGGA GGAGGTGCAG 
GCCGTGCAGC AGCAGGAGGT GTATGGCGCG ATGCCCAGGG ATGAATCTGA TTCCAAGACA 
GCCTCCCCTT GGAAGTCTGC TCGCCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTGA ACGAGCGCTG GCGGTCCCTG CAGCAGCTGG CTGAGGAGCG GAGCCAACTC 
TTGGGCAGTG CCCATGAAGT GCAGCGGTTC CACAGAGATG CTGACGAAAC CAAAGAGTGG 
ATCGAAGAGA AGAACCAGGC TCTAAACACA GACAACTACG GCCACGACCT GGCCAGTGTC 
CAGGCCCTGC AGCGCAAGCA CGAGGGCTTC GAGAGGGACC TCGCAGCCCT TGGTGACAAG 
GTGAACTCGC TCGGGGAGAC AGCCGAGCGC CTGATCCAGT CCCACCCCGA GTCGGCAGAG 
GACCTGCAGG AGAAGTGCAC TGAGCTGAAC CAGGCATGGA GCAGCCTGGG CAAGCGTGCC 
GACCAGCGCA AGGCCAAGCT GGGCGACTCC CACGACCTGC AGCGCTTCCT CAGCGACTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATC CGAGGTCTGG TGTCCTCAGA CGAGCTAGCC 
AAGGATGTCA CCGGAGCTGA GGCCCTGCTG GAGCGACACC AGGAACACCG GACAGAAATT 
GATGCCAGAG CTGGCACTTT CCAGGCATTC GAGCAGTTTG GGCAGCAGCT GTTGGCTCAT 
GGGCACTACG CCAGCCCCGA GATCAAGGAG AAGCTCGATG TTCTAGACCA GGAGCGTGCA 
GACCTTGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAATTGCAG 
CTGTTCCATC GGGATTGCGA GCAGGCCGAG AACTGGATGG CTGCCCGAGA GGCCTTTCTG 
AACACAGAAG ACAAAGGGGA CTCGCTAGAC AGTGTGGAGG CTCTGATCAA AAAACACGAG 
GACTTTGATA AAGCAATTAA TGTCCAGGAG GAGAAGATTG CTGCCCTGCA GTCCTTTGCT 
GACCAGCTCA TCGCTGGTGG CCACTACGCC AAAGGCGACA TCTCCAGCCG GCGCAATGAG 
GTTCTGGACA GGTGGCGCCG TCTAAAAGCT CAGATGATTG AGAAGAGGTC AAAGCTGGGA 
GAATCACAGA CCCTCCAGCA GTTCAGCCGG GATGTAGACG AGATTGAAGC TTGGATCAGT 
GAAAAATTAC AGACTGCAAG TGATGAGTCA TATAAGGACC CCACCAACAT CCAGCTTTCC 
AAGCTGCTGA GCAAGCATCA GAAGCACCAG GCCTTCGAGG CAGAACTGCA CGCCAATGCG 
GACCGGATCC GTGGGGTCAT CGACATGGGC AACTCCCTCA TTGACCGCGG GGCCTGCGCC 
GGCAGCGAGG ATGCCGTCAA GGCCCGGCTG GCCGCCTTAG CTGACCAGTG GCAGTTCCTG 
GTGCAGAAGT CGGCTGAGAA GAGCCAGAAG CTGAAAGAAG CCAACAAGCA GCAGAACTTC 
AACACTGGCA TCAAGGACTT CGACTTCTGG CTCTCTGAGG TGGAAGCCCT GCTGGCCTCT 
GAGGATTATG GCAAAGACCT GGCTTCTGTG AACAACTTGC TAAAAAAACA TCAGCTGCTG 
GAGGCAGATA TCTCTGCCCA CGAGGACCGC CTGAAGGACC TCAACAGCCA GGCGGACAGC 
CTGATGACCA GCAGCGCCTT CGACACCTCC CAGGTGAAGG ACAAGCGGGA CGCCATCAAC 
GGCCGTTTCC AGAAGATCAA GAGCATGGCC GCCTCGAGGC GAGCTAGGCT GAATGAGTCC 
CACCGCCTGC ACCAGTTTTT CCGGGACATG GATGATGAGG AGTCCTGGAT CAAGGAAAAG 
AAGCTGCTGG TGAGCTCAGA GGACTACGGC CGTGACCTGA CGGGCGTCCA GAACCTGAGG 
AAGAAGCACA AACGGCTGGA AGCAGAGCTG GCTGCCCACG AGCCGGCCAT TCAGGGTGTC 
CTGGACACTG GAAAGAAGCT GTCTGATGAC AACACCATCG GGAAGGAGGA GATTCAGCAG 
CGCCTGGCGC AGTTCGTGGA GCACTGGAAG GAGCTGAAGC AGCTGGCAGC TGCCCGGGGC 
CAGCGGCTAG AAGAATCTTT GGAATATCAG CAGTTTGTAG CCAACGTGGA GGAGGAGGAA 
GCCTGGATCA ATGAGAAGAT GACCCTGGTG GCCAGTGAAG ATTATGGAGA CACTCTTGCT 
GCCATCCAGG GCTTGCTGAA GAAACACGAA GCCTTCGAGA CGGACTTCAC TGTCCACAAG 
GACCGAGTGA ATGATGTCTG CACCAACGGC CAGGACCTGG TTAAGAAGAA CAATCACCAT 
GAGGAGAACA TCTCTTCCAA GATGAAGGGC TTGAACGGGA AAGTGTCAGA CTTGGAGAAG 
GCTGCCTCCC AGAGGAAGGC CAAGCTCGAC GAGAACTCGG CCTTCCTCCA GTTCAACTGG 
AAAGCCGATG TGGTGGAGTC CTGGATCGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT 
TATGGTCGAG ATCTGTCTTC TGTCCAAACT CTCCTCACCA AACAGGAAAC CTTCGATGCT 
GGCCTGCAGG CCTTCCAGCA GGAGGGCATC GCCAACATCA CTGCCCTCAA AGATCAGCTG 
CTGGCCGCCA AGCACGTTCA GTCCAAGGCC ATCGAGGCCC GCCATGCTGC CCTCATGAAG 
AGGTGGAGCC AGCTCCTGGC CAACTCGGCC ACCCGGAAGA AGAAGCTGCT GGAGGCACAG 
AGTCACTTCC GCAAGGTGGA GGATCTCTTC CTGACCTTTG CTAAAAAGGC TTCAGCCTTC 
AACAGCTGGT TTGAAAACGC AGAGGAGGAC TTAACCGACC CAGTGCGCTG CAACTCTCTG 
GAAGAGATCA AGGCCTTGAG AGAGGCCCAC GACGCCTTCC GCTCCTCGTT GAGCTCGGCC 
CAGGCCGACT TCAACCAGCT GGCAGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTGGCC 
TCCAACCCCT ACACCTGGTT CACCATGGAG GCCCTGGAGG AGACCTGGAG AAACCTACAG 
AAGATCATCA AGGAAAGGGA GCTGGAGCTG CAGAAGGAGC AGCGACGGCA GGAGGAGAAT 
GACAAGCTGC GCCAGGAGTT CGCCCAGCAT GCCAACGCCT TCCACCAGTG GATCCAGGAG 
ACCAGGTCCT GCATGGTGGA AGAATCCGGG ACCCTCGAAT CACAGCTTGA AGCTACCAAA 
CGCAAACACC AGGAGATTCG AGCTATGAGA AGTCAGCTCA AGAAGATTGA AGACCTGGGG 
GCGGCCATGG AGGAAGCGCT GATCCTGGAC AACAAGTACA CGGAGCACAG CACCGTGGGC 
CTGGCCCAGC AGTGGGACCA GCTCGACCAG TTGGGCATGC GCATGCAGCA CAACCTGGAG 
CAGCAGATCC AGGCCAGGAA CACGACCGGA GTGACCGAGG AGGCCCTCAA AGAATTCAGC 
ATGATGTTCA AACACTTCGA CAAGGACAAG TCTGGCCGGC TGAACCACCA GGAGTTCAAA 
TCCTGTCTGC GCTCCCTGGG CTATGACCTG CCCATGGTGG AGGAAGGGGA GCCCGACCCT 
GAGTTCGAGG CCATCCTGGA CACTGTGGAT CCTAACAGGG ACGGCCACGT CTCCCTGCAA 
GAGTACATGG CCTTTATGAT CAGCCGTGAG ACCGAGAACG TCAAGTCCAG CGAGGAGATT 
GAGAGCGCCT TCCGGGCCCT GAGCTCTGAA GGGAAGCCTT ATGTGACCAA GGAGGAGCTG 
TACCAGAACC TGACTCGGGA ACAAGCCGAC TACTGCGTCT CCCACATGAA GCCCTACGTG 
GACGGCAAGG GCCGCGAACT CCCCACTGCT TTTGACTACG TCGAGTTCAC CCGCTCGCTC 
TTCGTGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010808762.2
CDS	54..7505
Translation	MLSSFRKRRVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLHQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMYRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVDHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWESLKSKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRVTKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGAMPRDESDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKEKLDVLDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQSFADQLIAGGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDMGNSLIDRGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDAINGRFQKIKSMAASRRARLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLVKKNNHHEENISSKMKGLNGKVSDLEKAASQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHAALMKRWSQLLANSATRKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_010810460.2
Organism	Bos taurus(cattle)
Definition	Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010810460.2

ORF Insert Sequence:

ATGCTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGCGG GGTCAAAGTG 
CTGGAGACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTGGACAG ATACCACCGC 
TTCAAGGAGC TCTCGACCCT GAGGCGCCAG AAGCTTGAAG ACTCCTACCG ATTCCAGTTT 
TTCCAAAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAGCTTCA GATTGCCTCA 
GATGAGAATT ACAAAGACCC AACCAACCTG CAGGGCAAGC TGCAGAAGCA TCAAGCCTTC 
GAAGCCGAGG TGCAGGCCAA CTCGGGCGCC ATCGTGAAGC TGGATGAGAC TGGTAACCTG 
ATGATCTCAG AAGGGCACTT TGCATCCGAA ACCATCCGGA CTCGTCTGAT GGAGCTGCAC 
CGCCAGTGGG AATTGCTCCT GGAGAAGATG CGGGAGAAAG GCATCAAGCT GCTGCAGGCC 
CAGAAGCTGG TGCAGTACCT GCGGGAGTGT GAGGATGTGA TGGACTGGAT CAATGACAAG 
GAAGCCATTG TGACGTCTGA GGAGCTGGGC CAGGACCTGG AGCACGTGGA GGTGTTACAG 
AAGAAATTCG AAGAGTTTCA GACAGACATG GCTGCCCACG AAGAGAGAGT CAATGAAGTG 
AACCAGTTCG CTGCCAAGCT CATTCAGGAG CAGCACCCTG AGGAGGAGCT GATCAAGACA 
AAGCAGGACG AGGTGAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCCCT CCAGCGGCAG 
GGGAAGCTGT TCGGGGCAGC TGAGGTCCAA CGCTTTAACA GGGATGTGGA CGAGACCATC 
AGCTGGATTA AGGAGAAGGA ACAGTTAATG GCCTCTGATG ACTTTGGCCG GGACCTGGCC 
AGCGTCCAGG CTTTACTACG GAAACACGAG GGTTTGGAGA GAGATCTTGC TGCCTTGGAG 
GACAAGGTCA AAGCCCTGTG TGCCGAGGCT GACCGCCTGC ATCAGTCCCA CCCTCTGAGT 
GCCACCCAGA TCCAGGTGAA GCGAGAGGAG CTGATCACCA ACTGGGAGCA GATCCGCACC 
CTGGCCGCGG AGAGACACGC ACGTCTCAAC GATTCCTACA GGCTTCAGCG CTTCCTTGCC 
GACTTCCGAG ACCTCACCAG CTGGGTCACT GAGATGAAAG CCCTCATCAA CGCAGATGAA 
CTTGCCAACG ATGTGGCCGG AGCCGAAGCC CTGCTGGATA GGCATCAGGA GCACAAGGGT 
GAAATTGACG CCCATGAAGA TAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT 
GCAGCTGGTC ACTACGCCTC AGATGAAGTG AGGGAGAAGC TTACCGTGCT GTCCGAGGAG 
CGGGCCGCGC TGCTGGAGCT CTGGGAGCTG CGACGGCAGC AATATGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ACGAAGACCT GGGCGATTCC TTGGATAGTG TGGAAGCGCT TCTAAAGAAG 
CACGAAGACT TTGAGAAGTC CCTCAGCGCC CAGGAGGAGA AGATCACAGC CTTAGATGAG 
TTTGCCACCA AGCTGATTCA GAACAACCAC TACGCAATGG AAGATGTGGC CACCCGGCGA 
GACGCACTGC TGAGCCGTCG CAATGCCCTG CATGAGAGAG CCATGTACCG CCGTGCCCAG 
CTTGCAGATT CCTTCCACTT GCAGCAGTTT TTCCGTGACT CTGATGAGCT CAAGAGTTGG 
GTCAACGAGA AGATGAAAAC AGCCACGGAT GAAGCTTATA AAGACCCATC CAACCTACAA 
GGGAAAGTGC AGAAGCACCA GGCCTTTGAG GCCGAGCTCT CGGCAAACCA GAGCCGCATC 
GACGCCCTGG AGAAAGCCGG GCAGAAGCTG ATCGATGTCG ACCACTATGC CAAGGACGAG 
GTGGCGGCTC GGATGAATGA GGTCATCAGT CTGTGGAAGA AGCTGCTGGA GGCCACAGAG 
CTGAAAGGCA TAAAGCTCCG CGAGGCTAAC CAGCAGCAGC AGTTTAATCG CAACGTTGAG 
GACATCGAGC TGTGGCTGTA TGAAGTGGAA GGTCATCTGG CCTCGGACGA CTACGGCAAA 
GACCTCACCA ACGTCCAGAA CCTCCAGAAG AAGCACGCCC TGCTGGAGGC TGACGTGGCA 
GCTCACCAGG ATCGCATCGA TGGCATCACT ATCCAAGCCC GCCAGTTCCA GGACGCGGGC 
CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAGGCCC TGGTAGCTCG CTACGAGGCG 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCTG ATTCCCTGCG GTTGCAGCAG 
CTCTTCCGTG ACGTCGAGGA TGAGGAGACG TGGATTCGGG AGAAAGAACC CATTGCCGCC 
TCCACCAACA GAGGCAAAGA TTTAATTGGA GTCCAGAACC TGCTAAAGAA ACACCAAGCG 
CTACAAGCAG AAATTGCTGG CCACGAGCCC CGCATCAAAG CAGTCACACA GAAGGGGAAC 
GCCATGGTGG AGGAGGGCCA TTTTGCTGCT GAGGACGTGA AGGCCAAGCT CCATGAGCTG 
AACCAGAAGT GGGAGTCGCT GAAGTCCAAG GCCTCCCAGC GGCGGCAGGA CCTGGAGGAC 
TCGCTGCAGG CCCAGCAGTA CTTTGCCGAC GCCAACGAGG CTGAGTCCTG GATGCGGGAG 
AAGGAGCCCA TCGTGGGCAG CACCGACTAT GGCAAAGATG AGGACTCGGC CGAGGCTCTG 
CTAAAGAAGC ACGAGGCATT GATGTCGGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCC 
CTGCGAGAGC AGGCCCAGTC GTGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG 
AAGGAGCTGG TCTTGGCGCT CTATGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAAGGAG ACATTCTCAC CTTACTCAAC AGCACCAACA AGGACTGGTG GAAAGTGGAA 
GTGAATGACC GGCAGGGCTT CGTGCCGGCT GCCTACGTGA AGAAGCTGGA TCCTGCGCAG 
TCGGCCTCCC GGGAGAACCT CCTGGAAGAG CAGGGCAGCA TCGCGCTGCG GCAGGAACAG 
ATCGACAACC AGACGCGCGT AACTAAGGAG GCTGGCAGCG TGTCTCTGCG TATGAAACAG 
GTGGAAGAAC TGTACCATTC TCTGCTGGAA CTGGGAGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGCTGTA AGAAGTTCAT GTTGTTCCGT GAAGCGAACG AGCTACAGCA GTGGATCAAT 
GAGAAAGAAG CGGCCCTGAC AAGTGAGGAG GTTGGCGCGG ACTTGGAGCA GGTTGAAGTG 
CTGCAGAAGA AGTTTGACGA CTTCCAGAAG GATCTCAAGG CCAATGAGTC GCGGCTGAAG 
GACATTAACA AGGTGGCTGA AGACCTGGAG TCGGAAGGTC TGATGGCGGA GGAGGTGCAG 
GCCGTGCAGC AGCAGGAGGT GTATGGCGCG ATGCCCAGGG ATGAATCTGA TTCCAAGACA 
GCCTCCCCTT GGAAGTCTGC TCGCCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTGA ACGAGCGCTG GCGGTCCCTG CAGCAGCTGG CTGAGGAGCG GAGCCAACTC 
TTGGGCAGTG CCCATGAAGT GCAGCGGTTC CACAGAGATG CTGACGAAAC CAAAGAGTGG 
ATCGAAGAGA AGAACCAGGC TCTAAACACA GACAACTACG GCCACGACCT GGCCAGTGTC 
CAGGCCCTGC AGCGCAAGCA CGAGGGCTTC GAGAGGGACC TCGCAGCCCT TGGTGACAAG 
GTGAACTCGC TCGGGGAGAC AGCCGAGCGC CTGATCCAGT CCCACCCCGA GTCGGCAGAG 
GACCTGCAGG AGAAGTGCAC TGAGCTGAAC CAGGCATGGA GCAGCCTGGG CAAGCGTGCC 
GACCAGCGCA AGGCCAAGCT GGGCGACTCC CACGACCTGC AGCGCTTCCT CAGCGACTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATC CGAGGTCTGG TGTCCTCAGA CGAGCTAGCC 
AAGGATGTCA CCGGAGCTGA GGCCCTGCTG GAGCGACACC AGGAACACCG GACAGAAATT 
GATGCCAGAG CTGGCACTTT CCAGGCATTC GAGCAGTTTG GGCAGCAGCT GTTGGCTCAT 
GGGCACTACG CCAGCCCCGA GATCAAGGAG AAGCTCGATG TTCTAGACCA GGAGCGTGCA 
GACCTTGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAATTGCAG 
CTGTTCCATC GGGATTGCGA GCAGGCCGAG AACTGGATGG CTGCCCGAGA GGCCTTTCTG 
AACACAGAAG ACAAAGGGGA CTCGCTAGAC AGTGTGGAGG CTCTGATCAA AAAACACGAG 
GACTTTGATA AAGCAATTAA TGTCCAGGAG GAGAAGATTG CTGCCCTGCA GTCCTTTGCT 
GACCAGCTCA TCGCTGGTGG CCACTACGCC AAAGGCGACA TCTCCAGCCG GCGCAATGAG 
GTTCTGGACA GGTGGCGCCG TCTAAAAGCT CAGATGATTG AGAAGAGGTC AAAGCTGGGA 
GAATCACAGA CCCTCCAGCA GTTCAGCCGG GATGTAGACG AGATTGAAGC TTGGATCAGT 
GAAAAATTAC AGACTGCAAG TGATGAGTCA TATAAGGACC CCACCAACAT CCAGCTTTCC 
AAGCTGCTGA GCAAGCATCA GAAGCACCAG GCCTTCGAGG CAGAACTGCA CGCCAATGCG 
GACCGGATCC GTGGGGTCAT CGACATGGGC AACTCCCTCA TTGACCGCGG GGCCTGCGCC 
GGCAGCGAGG ATGCCGTCAA GGCCCGGCTG GCCGCCTTAG CTGACCAGTG GCAGTTCCTG 
GTGCAGAAGT CGGCTGAGAA GAGCCAGAAG CTGAAAGAAG CCAACAAGCA GCAGAACTTC 
AACACTGGCA TCAAGGACTT CGACTTCTGG CTCTCTGAGG TGGAAGCCCT GCTGGCCTCT 
GAGGATTATG GCAAAGACCT GGCTTCTGTG AACAACTTGC TAAAAAAACA TCAGCTGCTG 
GAGGCAGATA TCTCTGCCCA CGAGGACCGC CTGAAGGACC TCAACAGCCA GGCGGACAGC 
CTGATGACCA GCAGCGCCTT CGACACCTCC CAGGTGAAGG ACAAGCGGGA CGCCATCAAC 
GGCCGTTTCC AGAAGATCAA GAGCATGGCC GCCTCGAGGC GAGCTAGGCT GAATGAGTCC 
CACCGCCTGC ACCAGTTTTT CCGGGACATG GATGATGAGG AGTCCTGGAT CAAGGAAAAG 
AAGCTGCTGG TGAGCTCAGA GGACTACGGC CGTGACCTGA CGGGCGTCCA GAACCTGAGG 
AAGAAGCACA AACGGCTGGA AGCAGAGCTG GCTGCCCACG AGCCGGCCAT TCAGGGTGTC 
CTGGACACTG GAAAGAAGCT GTCTGATGAC AACACCATCG GGAAGGAGGA GATTCAGCAG 
CGCCTGGCGC AGTTCGTGGA GCACTGGAAG GAGCTGAAGC AGCTGGCAGC TGCCCGGGGC 
CAGCGGCTAG AAGAATCTTT GGAATATCAG CAGTTTGTAG CCAACGTGGA GGAGGAGGAA 
GCCTGGATCA ATGAGAAGAT GACCCTGGTG GCCAGTGAAG ATTATGGAGA CACTCTTGCT 
GCCATCCAGG GCTTGCTGAA GAAACACGAA GCCTTCGAGA CGGACTTCAC TGTCCACAAG 
GACCGAGTGA ATGATGTCTG CACCAACGGC CAGGACCTGG TTAAGAAGAA CAATCACCAT 
GAGGAGAACA TCTCTTCCAA GATGAAGGGC TTGAACGGGA AAGTGTCAGA CTTGGAGAAG 
GCTGCCTCCC AGAGGAAGGC CAAGCTCGAC GAGAACTCGG CCTTCCTCCA GTTCAACTGG 
AAAGCCGATG TGGTGGAGTC CTGGATCGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT 
TATGGTCGAG ATCTGTCTTC TGTCCAAACT CTCCTCACCA AACAGGAAAC CTTCGATGCT 
GGCCTGCAGG CCTTCCAGCA GGAGGGCATC GCCAACATCA CTGCCCTCAA AGATCAGCTG 
CTGGCCGCCA AGCACGTTCA GTCCAAGGCC ATCGAGGCCC GCCATGCTGC CCTCATGAAG 
AGGTGGAGCC AGCTCCTGGC CAACTCGGCC ACCCGGAAGA AGAAGCTGCT GGAGGCACAG 
AGTCACTTCC GCAAGGTGGA GGATCTCTTC CTGACCTTTG CTAAAAAGGC TTCAGCCTTC 
AACAGCTGGT TTGAAAACGC AGAGGAGGAC TTAACCGACC CAGTGCGCTG CAACTCTCTG 
GAAGAGATCA AGGCCTTGAG AGAGGCCCAC GACGCCTTCC GCTCCTCGTT GAGCTCGGCC 
CAGGCCGACT TCAACCAGCT GGCAGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTGGCC 
TCCAACCCCT ACACCTGGTT CACCATGGAG GCCCTGGAGG AGACCTGGAG AAACCTACAG 
AAGATCATCA AGGAAAGGGA GCTGGAGCTG CAGAAGGAGC AGCGACGGCA GGAGGAGAAT 
GACAAGCTGC GCCAGGAGTT CGCCCAGCAT GCCAACGCCT TCCACCAGTG GATCCAGGAG 
ACCAGGTCCT GCATGGTGGA AGAATCCGGG ACCCTCGAAT CACAGCTTGA AGCTACCAAA 
CGCAAACACC AGGAGATTCG AGCTATGAGA AGTCAGCTCA AGAAGATTGA AGACCTGGGG 
GCGGCCATGG AGGAAGCGCT GATCCTGGAC AACAAGTACA CGGAGCACAG CACCGTGGGC 
CTGGCCCAGC AGTGGGACCA GCTCGACCAG TTGGGCATGC GCATGCAGCA CAACCTGGAG 
CAGCAGATCC AGGCCAGGAA CACGACCGGA GTGACCGAGG AGGCCCTCAA AGAATTCAGC 
ATGATGTTCA AACACTTCGA CAAGGACAAG TCTGGCCGGC TGAACCACCA GGAGTTCAAA 
TCCTGTCTGC GCTCCCTGGG CTATGACCTG CCCATGGTGG AGGAAGGGGA GCCCGACCCT 
GAGTTCGAGG CCATCCTGGA CACTGTGGAT CCTAACAGGG ACGGCCACGT CTCCCTGCAA 
GAGTACATGG CCTTTATGAT CAGCCGTGAG ACCGAGAACG TCAAGTCCAG CGAGGAGATT 
GAGAGCGCCT TCCGGGCCCT GAGCTCTGAA GGGAAGCCTT ATGTGACCAA GGAGGAGCTG 
TACCAGAACC TGACTCGGGA ACAAGCCGAC TACTGCGTCT CCCACATGAA GCCCTACGTG 
GACGGCAAGG GCCGCGAACT CCCCACTGCT TTTGACTACG TCGAGTTCAC CCGCTCGCTC 
TTCGTGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( NM_001114156.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001107628.1 Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), mRNA.

Price & Availability↑

CloneID	OBa37323
Clone ID Related Accession (Same CDS sequence)	XM_015473654.1 , XM_024999035.1 , NM_001114156.1
Accession Version	NM_001114156.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7419bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-28
Organism	Bos taurus(cattle)
Product	spectrin alpha chain, non-erythrocytic 1
Comment	Comment: INFERRED REFSEQ: This record is predicted by genome sequence analysis and is not yet supported by experimental evidence. The reference sequence was derived from NKLS02000011.1. On Feb 1, 2008 this sequence version replaced XM_866848.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## CDS exon combination :: SRR5571260.49005.1, SRR6764281.2934.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145414 [ECO:0000348] ##Evidence-Data-END##

ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT 
GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT 
CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC 
AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG 
AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC 
CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG 
AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT 
GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC 
CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC 
CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC 
CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT 
AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT 
GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG 
GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC 
ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC 
TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG 
AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG 
GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA 
GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG 
AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG 
CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG 
GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA 
ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG 
AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT 
GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT 
TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG 
CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT 
GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG 
AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG 
CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT 
CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC 
GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA 
GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG 
GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT 
CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG 
AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC 
AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC 
GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC 
CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC 
GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA 
GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC 
GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC 
AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC 
GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC 
AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGACGC GCGTAACTAA GGAGGCTGGC 
AGCGTGTCTC TGCGTATGAA ACAGGTGGAA GAACTGTACC ATTCTCTGCT GGAACTGGGA 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG 
AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC 
GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC 
AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA 
GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC 
AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG 
CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA 
GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC 
TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG 
GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC 
CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA 
TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC 
CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT 
CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG 
TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC 
GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG 
ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG 
GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG 
ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC 
GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG 
ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA 
GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG 
GACCCCACCA ACATCCAGAG CAAGCATCAG AAGCACCAGG CCTTCGAGGC AGAACTGCAC 
GCCAATGCGG ACCGGATCCG TGGGGTCATC GACATGGGCA ACTCCCTCAT TGACCGCGGG 
GCCTGCGCCG GCAGCGAGGA TGCCGTCAAG GCCCGGCTGG CCGCCTTAGC TGACCAGTGG 
CAGTTCCTGG TGCAGAAGTC GGCTGAGAAG AGCCAGAAGC TGAAAGAAGC CAACAAGCAG 
CAGAACTTCA ACACTGGCAT CAAGGACTTC GACTTCTGGC TCTCTGAGGT GGAAGCCCTG 
CTGGCCTCTG AGGATTATGG CAAAGACCTG GCTTCTGTGA ACAACTTGCT AAAAAAACAT 
CAGCTGCTGG AGGCAGATAT CTCTGCCCAC GAGGACCGCC TGAAGGACCT CAACAGCCAG 
GCGGACAGCC TGATGACCAG CAGCGCCTTC GACACCTCCC AGGTGAAGGA CAAGCGGGAC 
GCCATCAACG GCCGTTTCCA GAAGATCAAG AGCATGGCCG CCTCGAGGCG AGCTAGGCTG 
AATGAGTCCC ACCGCCTGCA CCAGTTTTTC CGGGACATGG ATGATGAGGA GTCCTGGATC 
AAGGAAAAGA AGCTGCTGGT GAGCTCAGAG GACTACGGCC GTGACCTGAC GGGCGTCCAG 
AACCTGAGGA AGAAGCACAA ACGGCTGGAA GCAGAGCTGG CTGCCCACGA GCCGGCCATT 
CAGGGTGTCC TGGACACTGG AAAGAAGCTG TCTGATGACA ACACCATCGG GAAGGAGGAG 
ATTCAGCAGC GCCTGGCGCA GTTCGTGGAG CACTGGAAGG AGCTGAAGCA GCTGGCAGCT 
GCCCGGGGCC AGCGGCTAGA AGAATCTTTG GAATATCAGC AGTTTGTAGC CAACGTGGAG 
GAGGAGGAAG CCTGGATCAA TGAGAAGATG ACCCTGGTGG CCAGTGAAGA TTATGGAGAC 
ACTCTTGCTG CCATCCAGGG CTTGCTGAAG AAACACGAAG CCTTCGAGAC GGACTTCACT 
GTCCACAAGG ACCGAGTGAA TGATGTCTGC ACCAACGGCC AGGACCTGGT TAAGAAGAAC 
AATCACCATG AGGAGAACAT CTCTTCCAAG ATGAAGGGCT TGAACGGGAA AGTGTCAGAC 
TTGGAGAAGG CTGCCTCCCA GAGGAAGGCC AAGCTCGACG AGAACTCGGC CTTCCTCCAG 
TTCAACTGGA AAGCCGATGT GGTGGAGTCC TGGATCGGTG AAAAGGAGAA CAGCTTGAAG 
ACAGATGATT ATGGTCGAGA TCTGTCTTCT GTCCAAACTC TCCTCACCAA ACAGGAAACC 
TTCGATGCTG GCCTGCAGGC CTTCCAGCAG GAGGGCATCG CCAACATCAC TGCCCTCAAA 
GATCAGCTGC TGGCCGCCAA GCACGTTCAG TCCAAGGCCA TCGAGGCCCG CCATGCTGCC 
CTCATGAAGA GGTGGAGCCA GCTCCTGGCC AACTCGGCCA CCCGGAAGAA GAAGCTGCTG 
GAGGCACAGA GTCACTTCCG CAAGGTGGAG GATCTCTTCC TGACCTTTGC TAAAAAGGCT 
TCAGCCTTCA ACAGCTGGTT TGAAAACGCA GAGGAGGACT TAACCGACCC AGTGCGCTGC 
AACTCTCTGG AAGAGATCAA GGCCTTGAGA GAGGCCCACG ACGCCTTCCG CTCCTCGTTG 
AGCTCGGCCC AGGCCGACTT CAACCAGCTG GCAGAGCTGG ACCGCCAGAT CAAGAGCTTC 
CGCGTGGCCT CCAACCCCTA CACCTGGTTC ACCATGGAGG CCCTGGAGGA GACCTGGAGA 
AACCTACAGA AGATCATCAA GGAAAGGGAG CTGGAGCTGC AGAAGGAGCA GCGACGGCAG 
GAGGAGAATG ACAAGCTGCG CCAGGAGTTC GCCCAGCATG CCAACGCCTT CCACCAGTGG 
ATCCAGGAGA CCAGAACGTA CCTCCTCGAT GGGTCCTGCA TGGTGGAAGA ATCCGGGACC 
CTCGAATCAC AGCTTGAAGC TACCAAACGC AAACACCAGG AGATTCGAGC TATGAGAAGT 
CAGCTCAAGA AGATTGAAGA CCTGGGGGCG GCCATGGAGG AAGCGCTGAT CCTGGACAAC 
AAGTACACGG AGCACAGCAC CGTGGGCCTG GCCCAGCAGT GGGACCAGCT CGACCAGTTG 
GGCATGCGCA TGCAGCACAA CCTGGAGCAG CAGATCCAGG CCAGGAACAC GACCGGAGTG 
ACCGAGGAGG CCCTCAAAGA ATTCAGCATG ATGTTCAAAC ACTTCGACAA GGACAAGTCT 
GGCCGGCTGA ACCACCAGGA GTTCAAATCC TGTCTGCGCT CCCTGGGCTA TGACCTGCCC 
ATGGTGGAGG AAGGGGAGCC CGACCCTGAG TTCGAGGCCA TCCTGGACAC TGTGGATCCT 
AACAGGGACG GCCACGTCTC CCTGCAAGAG TACATGGCCT TTATGATCAG CCGTGAGACC 
GAGAACGTCA AGTCCAGCGA GGAGATTGAG AGCGCCTTCC GGGCCCTGAG CTCTGAAGGG 
AAGCCTTATG TGACCAAGGA GGAGCTGTAC CAGAACCTGA CTCGGGAACA AGCCGACTAC 
TGCGTCTCCC ACATGAAGCC CTACGTGGAC GGCAAGGGCC GCGAACTCCC CACTGCTTTT 
GACTACGTCG AGTTCACCCG CTCGCTCTTC GTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001107628.1
CDS	1..7419
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLHQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMYRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVDHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWESLKSKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRVTKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGAMPRDESDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKEKLDVLDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQSFADQLIAGGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQSKHQKHQAFEAELHANADRIRGVIDMGNSLIDRGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDAINGRFQKIKSMAASRRARLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLVKKNNHHEENISSKMKGLNGKVSDLEKAASQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHAALMKRWSQLLANSATRKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	NM_001114156.1
Organism	Bos taurus(cattle)
Definition	Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001114156.1

ORF Insert Sequence:

ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT 
GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT 
CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC 
AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG 
AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC 
CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG 
AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT 
GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC 
CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC 
CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC 
CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT 
AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT 
GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG 
GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC 
ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC 
TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG 
AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG 
GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA 
GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG 
AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG 
CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG 
GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA 
ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG 
AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT 
GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT 
TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG 
CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT 
GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG 
AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG 
CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT 
CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC 
GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA 
GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG 
GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT 
CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG 
AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC 
AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC 
GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC 
CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC 
GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA 
GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC 
GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC 
AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC 
GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC 
AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGACGC GCGTAACTAA GGAGGCTGGC 
AGCGTGTCTC TGCGTATGAA ACAGGTGGAA GAACTGTACC ATTCTCTGCT GGAACTGGGA 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG 
AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC 
GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC 
AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA 
GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC 
AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG 
CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA 
GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC 
TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG 
GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC 
CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA 
TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC 
CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT 
CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG 
TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC 
GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG 
ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG 
GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG 
ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC 
GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG 
ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA 
GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG 
GACCCCACCA ACATCCAGAG CAAGCATCAG AAGCACCAGG CCTTCGAGGC AGAACTGCAC 
GCCAATGCGG ACCGGATCCG TGGGGTCATC GACATGGGCA ACTCCCTCAT TGACCGCGGG 
GCCTGCGCCG GCAGCGAGGA TGCCGTCAAG GCCCGGCTGG CCGCCTTAGC TGACCAGTGG 
CAGTTCCTGG TGCAGAAGTC GGCTGAGAAG AGCCAGAAGC TGAAAGAAGC CAACAAGCAG 
CAGAACTTCA ACACTGGCAT CAAGGACTTC GACTTCTGGC TCTCTGAGGT GGAAGCCCTG 
CTGGCCTCTG AGGATTATGG CAAAGACCTG GCTTCTGTGA ACAACTTGCT AAAAAAACAT 
CAGCTGCTGG AGGCAGATAT CTCTGCCCAC GAGGACCGCC TGAAGGACCT CAACAGCCAG 
GCGGACAGCC TGATGACCAG CAGCGCCTTC GACACCTCCC AGGTGAAGGA CAAGCGGGAC 
GCCATCAACG GCCGTTTCCA GAAGATCAAG AGCATGGCCG CCTCGAGGCG AGCTAGGCTG 
AATGAGTCCC ACCGCCTGCA CCAGTTTTTC CGGGACATGG ATGATGAGGA GTCCTGGATC 
AAGGAAAAGA AGCTGCTGGT GAGCTCAGAG GACTACGGCC GTGACCTGAC GGGCGTCCAG 
AACCTGAGGA AGAAGCACAA ACGGCTGGAA GCAGAGCTGG CTGCCCACGA GCCGGCCATT 
CAGGGTGTCC TGGACACTGG AAAGAAGCTG TCTGATGACA ACACCATCGG GAAGGAGGAG 
ATTCAGCAGC GCCTGGCGCA GTTCGTGGAG CACTGGAAGG AGCTGAAGCA GCTGGCAGCT 
GCCCGGGGCC AGCGGCTAGA AGAATCTTTG GAATATCAGC AGTTTGTAGC CAACGTGGAG 
GAGGAGGAAG CCTGGATCAA TGAGAAGATG ACCCTGGTGG CCAGTGAAGA TTATGGAGAC 
ACTCTTGCTG CCATCCAGGG CTTGCTGAAG AAACACGAAG CCTTCGAGAC GGACTTCACT 
GTCCACAAGG ACCGAGTGAA TGATGTCTGC ACCAACGGCC AGGACCTGGT TAAGAAGAAC 
AATCACCATG AGGAGAACAT CTCTTCCAAG ATGAAGGGCT TGAACGGGAA AGTGTCAGAC 
TTGGAGAAGG CTGCCTCCCA GAGGAAGGCC AAGCTCGACG AGAACTCGGC CTTCCTCCAG 
TTCAACTGGA AAGCCGATGT GGTGGAGTCC TGGATCGGTG AAAAGGAGAA CAGCTTGAAG 
ACAGATGATT ATGGTCGAGA TCTGTCTTCT GTCCAAACTC TCCTCACCAA ACAGGAAACC 
TTCGATGCTG GCCTGCAGGC CTTCCAGCAG GAGGGCATCG CCAACATCAC TGCCCTCAAA 
GATCAGCTGC TGGCCGCCAA GCACGTTCAG TCCAAGGCCA TCGAGGCCCG CCATGCTGCC 
CTCATGAAGA GGTGGAGCCA GCTCCTGGCC AACTCGGCCA CCCGGAAGAA GAAGCTGCTG 
GAGGCACAGA GTCACTTCCG CAAGGTGGAG GATCTCTTCC TGACCTTTGC TAAAAAGGCT 
TCAGCCTTCA ACAGCTGGTT TGAAAACGCA GAGGAGGACT TAACCGACCC AGTGCGCTGC 
AACTCTCTGG AAGAGATCAA GGCCTTGAGA GAGGCCCACG ACGCCTTCCG CTCCTCGTTG 
AGCTCGGCCC AGGCCGACTT CAACCAGCTG GCAGAGCTGG ACCGCCAGAT CAAGAGCTTC 
CGCGTGGCCT CCAACCCCTA CACCTGGTTC ACCATGGAGG CCCTGGAGGA GACCTGGAGA 
AACCTACAGA AGATCATCAA GGAAAGGGAG CTGGAGCTGC AGAAGGAGCA GCGACGGCAG 
GAGGAGAATG ACAAGCTGCG CCAGGAGTTC GCCCAGCATG CCAACGCCTT CCACCAGTGG 
ATCCAGGAGA CCAGAACGTA CCTCCTCGAT GGGTCCTGCA TGGTGGAAGA ATCCGGGACC 
CTCGAATCAC AGCTTGAAGC TACCAAACGC AAACACCAGG AGATTCGAGC TATGAGAAGT 
CAGCTCAAGA AGATTGAAGA CCTGGGGGCG GCCATGGAGG AAGCGCTGAT CCTGGACAAC 
AAGTACACGG AGCACAGCAC CGTGGGCCTG GCCCAGCAGT GGGACCAGCT CGACCAGTTG 
GGCATGCGCA TGCAGCACAA CCTGGAGCAG CAGATCCAGG CCAGGAACAC GACCGGAGTG 
ACCGAGGAGG CCCTCAAAGA ATTCAGCATG ATGTTCAAAC ACTTCGACAA GGACAAGTCT 
GGCCGGCTGA ACCACCAGGA GTTCAAATCC TGTCTGCGCT CCCTGGGCTA TGACCTGCCC 
ATGGTGGAGG AAGGGGAGCC CGACCCTGAG TTCGAGGCCA TCCTGGACAC TGTGGATCCT 
AACAGGGACG GCCACGTCTC CCTGCAAGAG TACATGGCCT TTATGATCAG CCGTGAGACC 
GAGAACGTCA AGTCCAGCGA GGAGATTGAG AGCGCCTTCC GGGCCCTGAG CTCTGAAGGG 
AAGCCTTATG TGACCAAGGA GGAGCTGTAC CAGAACCTGA CTCGGGAACA AGCCGACTAC 
TGCGTCTCCC ACATGAAGCC CTACGTGGAC GGCAAGGGCC GCGAACTCCC CACTGCTTTT 
GACTACGTCG AGTTCACCCG CTCGCTCTTC GTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_005213345.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005213402.1 Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa24457
Clone ID Related Accession (Same CDS sequence)	XM_005213345.2 , XM_005213345.3 , XM_024999029.1
Accession Version	XM_005213345.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7497bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005213345.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT 
GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT 
CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC 
AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG 
AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC 
CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG 
AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT 
GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC 
CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC 
CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC 
CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT 
AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT 
GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG 
GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC 
ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC 
TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG 
AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG 
GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA 
GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG 
AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG 
CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG 
GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA 
ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG 
AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT 
GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT 
TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG 
CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT 
GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG 
AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG 
CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT 
CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC 
GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA 
GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG 
GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT 
CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG 
AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC 
AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC 
GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC 
CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC 
GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA 
GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC 
GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC 
AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC 
GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC 
AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGACGC GCGTAACTAA GGAGGCTGGC 
AGCGTGTCTC TGCGTATGAA ACAGGTGGAA GAACTGTACC ATTCTCTGCT GGAACTGGGA 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG 
AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC 
GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC 
AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA 
GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC 
AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG 
CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA 
GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC 
TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG 
GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC 
CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA 
TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC 
CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT 
CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG 
TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC 
GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG 
ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG 
GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG 
ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC 
GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG 
ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA 
GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG 
GACCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ATCAGAAGCA CCAGGCCTTC 
GAGGCAGAAC TGCACGCCAA TGCGGACCGG ATCCGTGGGG TCATCGACAT GGGCAACTCC 
CTCATTGACC GCGGGGCCTG CGCCGGCAGC GAGGATGCCG TCAAGGCCCG GCTGGCCGCC 
TTAGCTGACC AGTGGCAGTT CCTGGTGCAG AAGTCGGCTG AGAAGAGCCA GAAGCTGAAA 
GAAGCCAACA AGCAGCAGAA CTTCAACACT GGCATCAAGG ACTTCGACTT CTGGCTCTCT 
GAGGTGGAAG CCCTGCTGGC CTCTGAGGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC 
TTGCTAAAAA AACATCAGCT GCTGGAGGCA GATATCTCTG CCCACGAGGA CCGCCTGAAG 
GACCTCAACA GCCAGGCGGA CAGCCTGATG ACCAGCAGCG CCTTCGACAC CTCCCAGGTG 
AAGGACAAGC GGGACGCCAT CAACGGCCGT TTCCAGAAGA TCAAGAGCAT GGCCGCCTCG 
AGGCGAGCTA GGCTGAATGA GTCCCACCGC CTGCACCAGT TTTTCCGGGA CATGGATGAT 
GAGGAGTCCT GGATCAAGGA AAAGAAGCTG CTGGTGAGCT CAGAGGACTA CGGCCGTGAC 
CTGACGGGCG TCCAGAACCT GAGGAAGAAG CACAAACGGC TGGAAGCAGA GCTGGCTGCC 
CACGAGCCGG CCATTCAGGG TGTCCTGGAC ACTGGAAAGA AGCTGTCTGA TGACAACACC 
ATCGGGAAGG AGGAGATTCA GCAGCGCCTG GCGCAGTTCG TGGAGCACTG GAAGGAGCTG 
AAGCAGCTGG CAGCTGCCCG GGGCCAGCGG CTAGAAGAAT CTTTGGAATA TCAGCAGTTT 
GTAGCCAACG TGGAGGAGGA GGAAGCCTGG ATCAATGAGA AGATGACCCT GGTGGCCAGT 
GAAGATTATG GAGACACTCT TGCTGCCATC CAGGGCTTGC TGAAGAAACA CGAAGCCTTC 
GAGACGGACT TCACTGTCCA CAAGGACCGA GTGAATGATG TCTGCACCAA CGGCCAGGAC 
CTGGTTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CCAAGATGAA GGGCTTGAAC 
GGGAAAGTGT CAGACTTGGA GAAGGCTGCC TCCCAGAGGA AGGCCAAGCT CGACGAGAAC 
TCGGCCTTCC TCCAGTTCAA CTGGAAAGCC GATGTGGTGG AGTCCTGGAT CGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGT CGAGATCTGT CTTCTGTCCA AACTCTCCTC 
ACCAAACAGG AAACCTTCGA TGCTGGCCTG CAGGCCTTCC AGCAGGAGGG CATCGCCAAC 
ATCACTGCCC TCAAAGATCA GCTGCTGGCC GCCAAGCACG TTCAGTCCAA GGCCATCGAG 
GCCCGCCATG CTGCCCTCAT GAAGAGGTGG AGCCAGCTCC TGGCCAACTC GGCCACCCGG 
AAGAAGAAGC TGCTGGAGGC ACAGAGTCAC TTCCGCAAGG TGGAGGATCT CTTCCTGACC 
TTTGCTAAAA AGGCTTCAGC CTTCAACAGC TGGTTTGAAA ACGCAGAGGA GGACTTAACC 
GACCCAGTGC GCTGCAACTC TCTGGAAGAG ATCAAGGCCT TGAGAGAGGC CCACGACGCC 
TTCCGCTCCT CGTTGAGCTC GGCCCAGGCC GACTTCAACC AGCTGGCAGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT GGCCTCCAAC CCCTACACCT GGTTCACCAT GGAGGCCCTG 
GAGGAGACCT GGAGAAACCT ACAGAAGATC ATCAAGGAAA GGGAGCTGGA GCTGCAGAAG 
GAGCAGCGAC GGCAGGAGGA GAATGACAAG CTGCGCCAGG AGTTCGCCCA GCATGCCAAC 
GCCTTCCACC AGTGGATCCA GGAGACCAGA ACGTACCTCC TCGATGGCAT AGCGTATCGT 
CGGGTCATTC GTGTCTATCA GTATGAAGTT GGGGATGATC TGTCTGGAAG GTCCTGCATG 
GTGGAAGAAT CCGGGACCCT CGAATCACAG CTTGAAGCTA CCAAACGCAA ACACCAGGAG 
ATTCGAGCTA TGAGAAGTCA GCTCAAGAAG ATTGAAGACC TGGGGGCGGC CATGGAGGAA 
GCGCTGATCC TGGACAACAA GTACACGGAG CACAGCACCG TGGGCCTGGC CCAGCAGTGG 
GACCAGCTCG ACCAGTTGGG CATGCGCATG CAGCACAACC TGGAGCAGCA GATCCAGGCC 
AGGAACACGA CCGGAGTGAC CGAGGAGGCC CTCAAAGAAT TCAGCATGAT GTTCAAACAC 
TTCGACAAGG ACAAGTCTGG CCGGCTGAAC CACCAGGAGT TCAAATCCTG TCTGCGCTCC 
CTGGGCTATG ACCTGCCCAT GGTGGAGGAA GGGGAGCCCG ACCCTGAGTT CGAGGCCATC 
CTGGACACTG TGGATCCTAA CAGGGACGGC CACGTCTCCC TGCAAGAGTA CATGGCCTTT 
ATGATCAGCC GTGAGACCGA GAACGTCAAG TCCAGCGAGG AGATTGAGAG CGCCTTCCGG 
GCCCTGAGCT CTGAAGGGAA GCCTTATGTG ACCAAGGAGG AGCTGTACCA GAACCTGACT 
CGGGAACAAG CCGACTACTG CGTCTCCCAC ATGAAGCCCT ACGTGGACGG CAAGGGCCGC 
GAACTCCCCA CTGCTTTTGA CTACGTCGAG TTCACCCGCT CGCTCTTCGT GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005213402.1
CDS	122..7618
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLHQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMYRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVDHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWESLKSKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRVTKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGAMPRDESDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKEKLDVLDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQSFADQLIAGGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDMGNSLIDRGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDAINGRFQKIKSMAASRRARLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLVKKNNHHEENISSKMKGLNGKVSDLEKAASQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHAALMKRWSQLLANSATRKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGIAYRRVIRVYQYEVGDDLSGRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_005213345.2
Organism	Bos taurus(cattle)
Definition	Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005213345.2

ORF Insert Sequence:

ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT 
GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT 
CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC 
AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG 
AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC 
CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG 
AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT 
GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC 
CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC 
CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC 
CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT 
AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT 
GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG 
GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC 
ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC 
TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG 
AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG 
GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA 
GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG 
AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG 
CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG 
GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA 
ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG 
AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT 
GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT 
TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG 
CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT 
GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG 
AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG 
CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT 
CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC 
GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA 
GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG 
GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT 
CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG 
AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC 
AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC 
GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC 
CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC 
GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA 
GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC 
GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC 
AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC 
GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC 
AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGACGC GCGTAACTAA GGAGGCTGGC 
AGCGTGTCTC TGCGTATGAA ACAGGTGGAA GAACTGTACC ATTCTCTGCT GGAACTGGGA 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG 
AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC 
GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC 
AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA 
GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC 
AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG 
CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA 
GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC 
TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG 
GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC 
CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA 
TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC 
CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT 
CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG 
TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC 
GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG 
ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG 
GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG 
ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC 
GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG 
ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA 
GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG 
GACCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ATCAGAAGCA CCAGGCCTTC 
GAGGCAGAAC TGCACGCCAA TGCGGACCGG ATCCGTGGGG TCATCGACAT GGGCAACTCC 
CTCATTGACC GCGGGGCCTG CGCCGGCAGC GAGGATGCCG TCAAGGCCCG GCTGGCCGCC 
TTAGCTGACC AGTGGCAGTT CCTGGTGCAG AAGTCGGCTG AGAAGAGCCA GAAGCTGAAA 
GAAGCCAACA AGCAGCAGAA CTTCAACACT GGCATCAAGG ACTTCGACTT CTGGCTCTCT 
GAGGTGGAAG CCCTGCTGGC CTCTGAGGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC 
TTGCTAAAAA AACATCAGCT GCTGGAGGCA GATATCTCTG CCCACGAGGA CCGCCTGAAG 
GACCTCAACA GCCAGGCGGA CAGCCTGATG ACCAGCAGCG CCTTCGACAC CTCCCAGGTG 
AAGGACAAGC GGGACGCCAT CAACGGCCGT TTCCAGAAGA TCAAGAGCAT GGCCGCCTCG 
AGGCGAGCTA GGCTGAATGA GTCCCACCGC CTGCACCAGT TTTTCCGGGA CATGGATGAT 
GAGGAGTCCT GGATCAAGGA AAAGAAGCTG CTGGTGAGCT CAGAGGACTA CGGCCGTGAC 
CTGACGGGCG TCCAGAACCT GAGGAAGAAG CACAAACGGC TGGAAGCAGA GCTGGCTGCC 
CACGAGCCGG CCATTCAGGG TGTCCTGGAC ACTGGAAAGA AGCTGTCTGA TGACAACACC 
ATCGGGAAGG AGGAGATTCA GCAGCGCCTG GCGCAGTTCG TGGAGCACTG GAAGGAGCTG 
AAGCAGCTGG CAGCTGCCCG GGGCCAGCGG CTAGAAGAAT CTTTGGAATA TCAGCAGTTT 
GTAGCCAACG TGGAGGAGGA GGAAGCCTGG ATCAATGAGA AGATGACCCT GGTGGCCAGT 
GAAGATTATG GAGACACTCT TGCTGCCATC CAGGGCTTGC TGAAGAAACA CGAAGCCTTC 
GAGACGGACT TCACTGTCCA CAAGGACCGA GTGAATGATG TCTGCACCAA CGGCCAGGAC 
CTGGTTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CCAAGATGAA GGGCTTGAAC 
GGGAAAGTGT CAGACTTGGA GAAGGCTGCC TCCCAGAGGA AGGCCAAGCT CGACGAGAAC 
TCGGCCTTCC TCCAGTTCAA CTGGAAAGCC GATGTGGTGG AGTCCTGGAT CGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGT CGAGATCTGT CTTCTGTCCA AACTCTCCTC 
ACCAAACAGG AAACCTTCGA TGCTGGCCTG CAGGCCTTCC AGCAGGAGGG CATCGCCAAC 
ATCACTGCCC TCAAAGATCA GCTGCTGGCC GCCAAGCACG TTCAGTCCAA GGCCATCGAG 
GCCCGCCATG CTGCCCTCAT GAAGAGGTGG AGCCAGCTCC TGGCCAACTC GGCCACCCGG 
AAGAAGAAGC TGCTGGAGGC ACAGAGTCAC TTCCGCAAGG TGGAGGATCT CTTCCTGACC 
TTTGCTAAAA AGGCTTCAGC CTTCAACAGC TGGTTTGAAA ACGCAGAGGA GGACTTAACC 
GACCCAGTGC GCTGCAACTC TCTGGAAGAG ATCAAGGCCT TGAGAGAGGC CCACGACGCC 
TTCCGCTCCT CGTTGAGCTC GGCCCAGGCC GACTTCAACC AGCTGGCAGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT GGCCTCCAAC CCCTACACCT GGTTCACCAT GGAGGCCCTG 
GAGGAGACCT GGAGAAACCT ACAGAAGATC ATCAAGGAAA GGGAGCTGGA GCTGCAGAAG 
GAGCAGCGAC GGCAGGAGGA GAATGACAAG CTGCGCCAGG AGTTCGCCCA GCATGCCAAC 
GCCTTCCACC AGTGGATCCA GGAGACCAGA ACGTACCTCC TCGATGGCAT AGCGTATCGT 
CGGGTCATTC GTGTCTATCA GTATGAAGTT GGGGATGATC TGTCTGGAAG GTCCTGCATG 
GTGGAAGAAT CCGGGACCCT CGAATCACAG CTTGAAGCTA CCAAACGCAA ACACCAGGAG 
ATTCGAGCTA TGAGAAGTCA GCTCAAGAAG ATTGAAGACC TGGGGGCGGC CATGGAGGAA 
GCGCTGATCC TGGACAACAA GTACACGGAG CACAGCACCG TGGGCCTGGC CCAGCAGTGG 
GACCAGCTCG ACCAGTTGGG CATGCGCATG CAGCACAACC TGGAGCAGCA GATCCAGGCC 
AGGAACACGA CCGGAGTGAC CGAGGAGGCC CTCAAAGAAT TCAGCATGAT GTTCAAACAC 
TTCGACAAGG ACAAGTCTGG CCGGCTGAAC CACCAGGAGT TCAAATCCTG TCTGCGCTCC 
CTGGGCTATG ACCTGCCCAT GGTGGAGGAA GGGGAGCCCG ACCCTGAGTT CGAGGCCATC 
CTGGACACTG TGGATCCTAA CAGGGACGGC CACGTCTCCC TGCAAGAGTA CATGGCCTTT 
ATGATCAGCC GTGAGACCGA GAACGTCAAG TCCAGCGAGG AGATTGAGAG CGCCTTCCGG 
GCCCTGAGCT CTGAAGGGAA GCCTTATGTG ACCAAGGAGG AGCTGTACCA GAACCTGACT 
CGGGAACAAG CCGACTACTG CGTCTCCCAC ATGAAGCCCT ACGTGGACGG CAAGGGCCGC 
GAACTCCCCA CTGCTTTTGA CTACGTCGAG TTCACCCGCT CGCTCTTCGT GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_005213345.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005213402.1 Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa24457
Clone ID Related Accession (Same CDS sequence)	XM_005213345.2 , XM_005213345.3 , XM_024999029.1
Accession Version	XM_005213345.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7497bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005213345.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT 
GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT 
CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC 
AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG 
AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC 
CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG 
AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT 
GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC 
CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC 
CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC 
CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT 
AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT 
GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG 
GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC 
ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC 
TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG 
AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG 
GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA 
GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG 
AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG 
CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG 
GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA 
ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG 
AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT 
GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT 
TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG 
CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT 
GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG 
AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG 
CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT 
CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC 
GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA 
GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG 
GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT 
CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG 
AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC 
AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC 
GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC 
CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC 
GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA 
GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC 
GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC 
AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC 
GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC 
AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGACGC GCGTAACTAA GGAGGCTGGC 
AGCGTGTCTC TGCGTATGAA ACAGGTGGAA GAACTGTACC ATTCTCTGCT GGAACTGGGA 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG 
AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC 
GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC 
AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA 
GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC 
AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG 
CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA 
GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC 
TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG 
GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC 
CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA 
TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC 
CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT 
CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG 
TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC 
GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG 
ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG 
GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG 
ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC 
GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG 
ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA 
GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG 
GACCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ATCAGAAGCA CCAGGCCTTC 
GAGGCAGAAC TGCACGCCAA TGCGGACCGG ATCCGTGGGG TCATCGACAT GGGCAACTCC 
CTCATTGACC GCGGGGCCTG CGCCGGCAGC GAGGATGCCG TCAAGGCCCG GCTGGCCGCC 
TTAGCTGACC AGTGGCAGTT CCTGGTGCAG AAGTCGGCTG AGAAGAGCCA GAAGCTGAAA 
GAAGCCAACA AGCAGCAGAA CTTCAACACT GGCATCAAGG ACTTCGACTT CTGGCTCTCT 
GAGGTGGAAG CCCTGCTGGC CTCTGAGGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC 
TTGCTAAAAA AACATCAGCT GCTGGAGGCA GATATCTCTG CCCACGAGGA CCGCCTGAAG 
GACCTCAACA GCCAGGCGGA CAGCCTGATG ACCAGCAGCG CCTTCGACAC CTCCCAGGTG 
AAGGACAAGC GGGACGCCAT CAACGGCCGT TTCCAGAAGA TCAAGAGCAT GGCCGCCTCG 
AGGCGAGCTA GGCTGAATGA GTCCCACCGC CTGCACCAGT TTTTCCGGGA CATGGATGAT 
GAGGAGTCCT GGATCAAGGA AAAGAAGCTG CTGGTGAGCT CAGAGGACTA CGGCCGTGAC 
CTGACGGGCG TCCAGAACCT GAGGAAGAAG CACAAACGGC TGGAAGCAGA GCTGGCTGCC 
CACGAGCCGG CCATTCAGGG TGTCCTGGAC ACTGGAAAGA AGCTGTCTGA TGACAACACC 
ATCGGGAAGG AGGAGATTCA GCAGCGCCTG GCGCAGTTCG TGGAGCACTG GAAGGAGCTG 
AAGCAGCTGG CAGCTGCCCG GGGCCAGCGG CTAGAAGAAT CTTTGGAATA TCAGCAGTTT 
GTAGCCAACG TGGAGGAGGA GGAAGCCTGG ATCAATGAGA AGATGACCCT GGTGGCCAGT 
GAAGATTATG GAGACACTCT TGCTGCCATC CAGGGCTTGC TGAAGAAACA CGAAGCCTTC 
GAGACGGACT TCACTGTCCA CAAGGACCGA GTGAATGATG TCTGCACCAA CGGCCAGGAC 
CTGGTTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CCAAGATGAA GGGCTTGAAC 
GGGAAAGTGT CAGACTTGGA GAAGGCTGCC TCCCAGAGGA AGGCCAAGCT CGACGAGAAC 
TCGGCCTTCC TCCAGTTCAA CTGGAAAGCC GATGTGGTGG AGTCCTGGAT CGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGT CGAGATCTGT CTTCTGTCCA AACTCTCCTC 
ACCAAACAGG AAACCTTCGA TGCTGGCCTG CAGGCCTTCC AGCAGGAGGG CATCGCCAAC 
ATCACTGCCC TCAAAGATCA GCTGCTGGCC GCCAAGCACG TTCAGTCCAA GGCCATCGAG 
GCCCGCCATG CTGCCCTCAT GAAGAGGTGG AGCCAGCTCC TGGCCAACTC GGCCACCCGG 
AAGAAGAAGC TGCTGGAGGC ACAGAGTCAC TTCCGCAAGG TGGAGGATCT CTTCCTGACC 
TTTGCTAAAA AGGCTTCAGC CTTCAACAGC TGGTTTGAAA ACGCAGAGGA GGACTTAACC 
GACCCAGTGC GCTGCAACTC TCTGGAAGAG ATCAAGGCCT TGAGAGAGGC CCACGACGCC 
TTCCGCTCCT CGTTGAGCTC GGCCCAGGCC GACTTCAACC AGCTGGCAGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT GGCCTCCAAC CCCTACACCT GGTTCACCAT GGAGGCCCTG 
GAGGAGACCT GGAGAAACCT ACAGAAGATC ATCAAGGAAA GGGAGCTGGA GCTGCAGAAG 
GAGCAGCGAC GGCAGGAGGA GAATGACAAG CTGCGCCAGG AGTTCGCCCA GCATGCCAAC 
GCCTTCCACC AGTGGATCCA GGAGACCAGA ACGTACCTCC TCGATGGCAT AGCGTATCGT 
CGGGTCATTC GTGTCTATCA GTATGAAGTT GGGGATGATC TGTCTGGAAG GTCCTGCATG 
GTGGAAGAAT CCGGGACCCT CGAATCACAG CTTGAAGCTA CCAAACGCAA ACACCAGGAG 
ATTCGAGCTA TGAGAAGTCA GCTCAAGAAG ATTGAAGACC TGGGGGCGGC CATGGAGGAA 
GCGCTGATCC TGGACAACAA GTACACGGAG CACAGCACCG TGGGCCTGGC CCAGCAGTGG 
GACCAGCTCG ACCAGTTGGG CATGCGCATG CAGCACAACC TGGAGCAGCA GATCCAGGCC 
AGGAACACGA CCGGAGTGAC CGAGGAGGCC CTCAAAGAAT TCAGCATGAT GTTCAAACAC 
TTCGACAAGG ACAAGTCTGG CCGGCTGAAC CACCAGGAGT TCAAATCCTG TCTGCGCTCC 
CTGGGCTATG ACCTGCCCAT GGTGGAGGAA GGGGAGCCCG ACCCTGAGTT CGAGGCCATC 
CTGGACACTG TGGATCCTAA CAGGGACGGC CACGTCTCCC TGCAAGAGTA CATGGCCTTT 
ATGATCAGCC GTGAGACCGA GAACGTCAAG TCCAGCGAGG AGATTGAGAG CGCCTTCCGG 
GCCCTGAGCT CTGAAGGGAA GCCTTATGTG ACCAAGGAGG AGCTGTACCA GAACCTGACT 
CGGGAACAAG CCGACTACTG CGTCTCCCAC ATGAAGCCCT ACGTGGACGG CAAGGGCCGC 
GAACTCCCCA CTGCTTTTGA CTACGTCGAG TTCACCCGCT CGCTCTTCGT GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005213402.1
CDS	121..7617
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLHQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMYRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVDHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWESLKSKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRVTKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGAMPRDESDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKEKLDVLDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQSFADQLIAGGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDMGNSLIDRGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDAINGRFQKIKSMAASRRARLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLVKKNNHHEENISSKMKGLNGKVSDLEKAASQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHAALMKRWSQLLANSATRKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGIAYRRVIRVYQYEVGDDLSGRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_005213345.3
Organism	Bos taurus(cattle)
Definition	Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005213345.3

ORF Insert Sequence:

ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT 
GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT 
CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC 
AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG 
AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC 
CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG 
AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT 
GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC 
CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC 
CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC 
CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT 
AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT 
GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG 
GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC 
ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC 
TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG 
AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG 
GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA 
GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG 
AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG 
CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG 
GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA 
ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG 
AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT 
GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT 
TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG 
CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT 
GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG 
AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG 
CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT 
CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC 
GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA 
GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG 
GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT 
CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG 
AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC 
AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC 
GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC 
CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC 
GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA 
GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC 
GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC 
AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC 
GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC 
AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGACGC GCGTAACTAA GGAGGCTGGC 
AGCGTGTCTC TGCGTATGAA ACAGGTGGAA GAACTGTACC ATTCTCTGCT GGAACTGGGA 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG 
AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC 
GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC 
AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA 
GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC 
AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG 
CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA 
GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC 
TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG 
GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC 
CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA 
TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC 
CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT 
CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG 
TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC 
GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG 
ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG 
GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG 
ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC 
GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG 
ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA 
GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG 
GACCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ATCAGAAGCA CCAGGCCTTC 
GAGGCAGAAC TGCACGCCAA TGCGGACCGG ATCCGTGGGG TCATCGACAT GGGCAACTCC 
CTCATTGACC GCGGGGCCTG CGCCGGCAGC GAGGATGCCG TCAAGGCCCG GCTGGCCGCC 
TTAGCTGACC AGTGGCAGTT CCTGGTGCAG AAGTCGGCTG AGAAGAGCCA GAAGCTGAAA 
GAAGCCAACA AGCAGCAGAA CTTCAACACT GGCATCAAGG ACTTCGACTT CTGGCTCTCT 
GAGGTGGAAG CCCTGCTGGC CTCTGAGGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC 
TTGCTAAAAA AACATCAGCT GCTGGAGGCA GATATCTCTG CCCACGAGGA CCGCCTGAAG 
GACCTCAACA GCCAGGCGGA CAGCCTGATG ACCAGCAGCG CCTTCGACAC CTCCCAGGTG 
AAGGACAAGC GGGACGCCAT CAACGGCCGT TTCCAGAAGA TCAAGAGCAT GGCCGCCTCG 
AGGCGAGCTA GGCTGAATGA GTCCCACCGC CTGCACCAGT TTTTCCGGGA CATGGATGAT 
GAGGAGTCCT GGATCAAGGA AAAGAAGCTG CTGGTGAGCT CAGAGGACTA CGGCCGTGAC 
CTGACGGGCG TCCAGAACCT GAGGAAGAAG CACAAACGGC TGGAAGCAGA GCTGGCTGCC 
CACGAGCCGG CCATTCAGGG TGTCCTGGAC ACTGGAAAGA AGCTGTCTGA TGACAACACC 
ATCGGGAAGG AGGAGATTCA GCAGCGCCTG GCGCAGTTCG TGGAGCACTG GAAGGAGCTG 
AAGCAGCTGG CAGCTGCCCG GGGCCAGCGG CTAGAAGAAT CTTTGGAATA TCAGCAGTTT 
GTAGCCAACG TGGAGGAGGA GGAAGCCTGG ATCAATGAGA AGATGACCCT GGTGGCCAGT 
GAAGATTATG GAGACACTCT TGCTGCCATC CAGGGCTTGC TGAAGAAACA CGAAGCCTTC 
GAGACGGACT TCACTGTCCA CAAGGACCGA GTGAATGATG TCTGCACCAA CGGCCAGGAC 
CTGGTTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CCAAGATGAA GGGCTTGAAC 
GGGAAAGTGT CAGACTTGGA GAAGGCTGCC TCCCAGAGGA AGGCCAAGCT CGACGAGAAC 
TCGGCCTTCC TCCAGTTCAA CTGGAAAGCC GATGTGGTGG AGTCCTGGAT CGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGT CGAGATCTGT CTTCTGTCCA AACTCTCCTC 
ACCAAACAGG AAACCTTCGA TGCTGGCCTG CAGGCCTTCC AGCAGGAGGG CATCGCCAAC 
ATCACTGCCC TCAAAGATCA GCTGCTGGCC GCCAAGCACG TTCAGTCCAA GGCCATCGAG 
GCCCGCCATG CTGCCCTCAT GAAGAGGTGG AGCCAGCTCC TGGCCAACTC GGCCACCCGG 
AAGAAGAAGC TGCTGGAGGC ACAGAGTCAC TTCCGCAAGG TGGAGGATCT CTTCCTGACC 
TTTGCTAAAA AGGCTTCAGC CTTCAACAGC TGGTTTGAAA ACGCAGAGGA GGACTTAACC 
GACCCAGTGC GCTGCAACTC TCTGGAAGAG ATCAAGGCCT TGAGAGAGGC CCACGACGCC 
TTCCGCTCCT CGTTGAGCTC GGCCCAGGCC GACTTCAACC AGCTGGCAGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT GGCCTCCAAC CCCTACACCT GGTTCACCAT GGAGGCCCTG 
GAGGAGACCT GGAGAAACCT ACAGAAGATC ATCAAGGAAA GGGAGCTGGA GCTGCAGAAG 
GAGCAGCGAC GGCAGGAGGA GAATGACAAG CTGCGCCAGG AGTTCGCCCA GCATGCCAAC 
GCCTTCCACC AGTGGATCCA GGAGACCAGA ACGTACCTCC TCGATGGCAT AGCGTATCGT 
CGGGTCATTC GTGTCTATCA GTATGAAGTT GGGGATGATC TGTCTGGAAG GTCCTGCATG 
GTGGAAGAAT CCGGGACCCT CGAATCACAG CTTGAAGCTA CCAAACGCAA ACACCAGGAG 
ATTCGAGCTA TGAGAAGTCA GCTCAAGAAG ATTGAAGACC TGGGGGCGGC CATGGAGGAA 
GCGCTGATCC TGGACAACAA GTACACGGAG CACAGCACCG TGGGCCTGGC CCAGCAGTGG 
GACCAGCTCG ACCAGTTGGG CATGCGCATG CAGCACAACC TGGAGCAGCA GATCCAGGCC 
AGGAACACGA CCGGAGTGAC CGAGGAGGCC CTCAAAGAAT TCAGCATGAT GTTCAAACAC 
TTCGACAAGG ACAAGTCTGG CCGGCTGAAC CACCAGGAGT TCAAATCCTG TCTGCGCTCC 
CTGGGCTATG ACCTGCCCAT GGTGGAGGAA GGGGAGCCCG ACCCTGAGTT CGAGGCCATC 
CTGGACACTG TGGATCCTAA CAGGGACGGC CACGTCTCCC TGCAAGAGTA CATGGCCTTT 
ATGATCAGCC GTGAGACCGA GAACGTCAAG TCCAGCGAGG AGATTGAGAG CGCCTTCCGG 
GCCCTGAGCT CTGAAGGGAA GCCTTATGTG ACCAAGGAGG AGCTGTACCA GAACCTGACT 
CGGGAACAAG CCGACTACTG CGTCTCCCAC ATGAAGCCCT ACGTGGACGG CAAGGGCCGC 
GAACTCCCCA CTGCTTTTGA CTACGTCGAG TTCACCCGCT CGCTCTTCGT GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_024999029.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854797.1 Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa24457
Clone ID Related Accession (Same CDS sequence)	XM_005213345.2 , XM_005213345.3 , XM_024999029.1
Accession Version	XM_024999029.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7497bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT 
GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT 
CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC 
AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG 
AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC 
CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG 
AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT 
GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC 
CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC 
CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC 
CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT 
AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT 
GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG 
GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC 
ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC 
TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG 
AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG 
GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA 
GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG 
AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG 
CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG 
GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA 
ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG 
AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT 
GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT 
TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG 
CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT 
GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG 
AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG 
CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT 
CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC 
GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA 
GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG 
GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT 
CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG 
AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC 
AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC 
GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC 
CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC 
GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA 
GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC 
GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC 
AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC 
GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC 
AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGACGC GCGTAACTAA GGAGGCTGGC 
AGCGTGTCTC TGCGTATGAA ACAGGTGGAA GAACTGTACC ATTCTCTGCT GGAACTGGGA 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG 
AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC 
GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC 
AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA 
GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC 
AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG 
CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA 
GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC 
TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG 
GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC 
CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA 
TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC 
CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT 
CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG 
TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC 
GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG 
ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG 
GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG 
ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC 
GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG 
ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA 
GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG 
GACCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ATCAGAAGCA CCAGGCCTTC 
GAGGCAGAAC TGCACGCCAA TGCGGACCGG ATCCGTGGGG TCATCGACAT GGGCAACTCC 
CTCATTGACC GCGGGGCCTG CGCCGGCAGC GAGGATGCCG TCAAGGCCCG GCTGGCCGCC 
TTAGCTGACC AGTGGCAGTT CCTGGTGCAG AAGTCGGCTG AGAAGAGCCA GAAGCTGAAA 
GAAGCCAACA AGCAGCAGAA CTTCAACACT GGCATCAAGG ACTTCGACTT CTGGCTCTCT 
GAGGTGGAAG CCCTGCTGGC CTCTGAGGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC 
TTGCTAAAAA AACATCAGCT GCTGGAGGCA GATATCTCTG CCCACGAGGA CCGCCTGAAG 
GACCTCAACA GCCAGGCGGA CAGCCTGATG ACCAGCAGCG CCTTCGACAC CTCCCAGGTG 
AAGGACAAGC GGGACGCCAT CAACGGCCGT TTCCAGAAGA TCAAGAGCAT GGCCGCCTCG 
AGGCGAGCTA GGCTGAATGA GTCCCACCGC CTGCACCAGT TTTTCCGGGA CATGGATGAT 
GAGGAGTCCT GGATCAAGGA AAAGAAGCTG CTGGTGAGCT CAGAGGACTA CGGCCGTGAC 
CTGACGGGCG TCCAGAACCT GAGGAAGAAG CACAAACGGC TGGAAGCAGA GCTGGCTGCC 
CACGAGCCGG CCATTCAGGG TGTCCTGGAC ACTGGAAAGA AGCTGTCTGA TGACAACACC 
ATCGGGAAGG AGGAGATTCA GCAGCGCCTG GCGCAGTTCG TGGAGCACTG GAAGGAGCTG 
AAGCAGCTGG CAGCTGCCCG GGGCCAGCGG CTAGAAGAAT CTTTGGAATA TCAGCAGTTT 
GTAGCCAACG TGGAGGAGGA GGAAGCCTGG ATCAATGAGA AGATGACCCT GGTGGCCAGT 
GAAGATTATG GAGACACTCT TGCTGCCATC CAGGGCTTGC TGAAGAAACA CGAAGCCTTC 
GAGACGGACT TCACTGTCCA CAAGGACCGA GTGAATGATG TCTGCACCAA CGGCCAGGAC 
CTGGTTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CCAAGATGAA GGGCTTGAAC 
GGGAAAGTGT CAGACTTGGA GAAGGCTGCC TCCCAGAGGA AGGCCAAGCT CGACGAGAAC 
TCGGCCTTCC TCCAGTTCAA CTGGAAAGCC GATGTGGTGG AGTCCTGGAT CGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGT CGAGATCTGT CTTCTGTCCA AACTCTCCTC 
ACCAAACAGG AAACCTTCGA TGCTGGCCTG CAGGCCTTCC AGCAGGAGGG CATCGCCAAC 
ATCACTGCCC TCAAAGATCA GCTGCTGGCC GCCAAGCACG TTCAGTCCAA GGCCATCGAG 
GCCCGCCATG CTGCCCTCAT GAAGAGGTGG AGCCAGCTCC TGGCCAACTC GGCCACCCGG 
AAGAAGAAGC TGCTGGAGGC ACAGAGTCAC TTCCGCAAGG TGGAGGATCT CTTCCTGACC 
TTTGCTAAAA AGGCTTCAGC CTTCAACAGC TGGTTTGAAA ACGCAGAGGA GGACTTAACC 
GACCCAGTGC GCTGCAACTC TCTGGAAGAG ATCAAGGCCT TGAGAGAGGC CCACGACGCC 
TTCCGCTCCT CGTTGAGCTC GGCCCAGGCC GACTTCAACC AGCTGGCAGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT GGCCTCCAAC CCCTACACCT GGTTCACCAT GGAGGCCCTG 
GAGGAGACCT GGAGAAACCT ACAGAAGATC ATCAAGGAAA GGGAGCTGGA GCTGCAGAAG 
GAGCAGCGAC GGCAGGAGGA GAATGACAAG CTGCGCCAGG AGTTCGCCCA GCATGCCAAC 
GCCTTCCACC AGTGGATCCA GGAGACCAGA ACGTACCTCC TCGATGGCAT AGCGTATCGT 
CGGGTCATTC GTGTCTATCA GTATGAAGTT GGGGATGATC TGTCTGGAAG GTCCTGCATG 
GTGGAAGAAT CCGGGACCCT CGAATCACAG CTTGAAGCTA CCAAACGCAA ACACCAGGAG 
ATTCGAGCTA TGAGAAGTCA GCTCAAGAAG ATTGAAGACC TGGGGGCGGC CATGGAGGAA 
GCGCTGATCC TGGACAACAA GTACACGGAG CACAGCACCG TGGGCCTGGC CCAGCAGTGG 
GACCAGCTCG ACCAGTTGGG CATGCGCATG CAGCACAACC TGGAGCAGCA GATCCAGGCC 
AGGAACACGA CCGGAGTGAC CGAGGAGGCC CTCAAAGAAT TCAGCATGAT GTTCAAACAC 
TTCGACAAGG ACAAGTCTGG CCGGCTGAAC CACCAGGAGT TCAAATCCTG TCTGCGCTCC 
CTGGGCTATG ACCTGCCCAT GGTGGAGGAA GGGGAGCCCG ACCCTGAGTT CGAGGCCATC 
CTGGACACTG TGGATCCTAA CAGGGACGGC CACGTCTCCC TGCAAGAGTA CATGGCCTTT 
ATGATCAGCC GTGAGACCGA GAACGTCAAG TCCAGCGAGG AGATTGAGAG CGCCTTCCGG 
GCCCTGAGCT CTGAAGGGAA GCCTTATGTG ACCAAGGAGG AGCTGTACCA GAACCTGACT 
CGGGAACAAG CCGACTACTG CGTCTCCCAC ATGAAGCCCT ACGTGGACGG CAAGGGCCGC 
GAACTCCCCA CTGCTTTTGA CTACGTCGAG TTCACCCGCT CGCTCTTCGT GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854797.1
CDS	25..7521
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLHQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMYRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVDHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWESLKSKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRVTKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGAMPRDESDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKEKLDVLDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQSFADQLIAGGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDMGNSLIDRGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDAINGRFQKIKSMAASRRARLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLVKKNNHHEENISSKMKGLNGKVSDLEKAASQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHAALMKRWSQLLANSATRKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGIAYRRVIRVYQYEVGDDLSGRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_024999029.1
Organism	Bos taurus(cattle)
Definition	Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999029.1

ORF Insert Sequence:

ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT 
GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT 
CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC 
AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG 
AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC 
CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG 
AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT 
GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC 
CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC 
CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC 
CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT 
AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT 
GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG 
GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC 
ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC 
TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG 
AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG 
GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA 
GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG 
AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG 
CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG 
GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA 
ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG 
AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT 
GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT 
TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG 
CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT 
GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG 
AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG 
CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT 
CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC 
GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA 
GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG 
GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT 
CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG 
AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC 
AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC 
GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC 
CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC 
GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA 
GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC 
GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC 
AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC 
GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC 
AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGACGC GCGTAACTAA GGAGGCTGGC 
AGCGTGTCTC TGCGTATGAA ACAGGTGGAA GAACTGTACC ATTCTCTGCT GGAACTGGGA 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG 
AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC 
GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC 
AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA 
GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC 
AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG 
CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA 
GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC 
TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG 
GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC 
CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA 
TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC 
CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT 
CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG 
TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC 
GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG 
ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG 
GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG 
ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC 
GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG 
ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA 
GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG 
GACCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ATCAGAAGCA CCAGGCCTTC 
GAGGCAGAAC TGCACGCCAA TGCGGACCGG ATCCGTGGGG TCATCGACAT GGGCAACTCC 
CTCATTGACC GCGGGGCCTG CGCCGGCAGC GAGGATGCCG TCAAGGCCCG GCTGGCCGCC 
TTAGCTGACC AGTGGCAGTT CCTGGTGCAG AAGTCGGCTG AGAAGAGCCA GAAGCTGAAA 
GAAGCCAACA AGCAGCAGAA CTTCAACACT GGCATCAAGG ACTTCGACTT CTGGCTCTCT 
GAGGTGGAAG CCCTGCTGGC CTCTGAGGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC 
TTGCTAAAAA AACATCAGCT GCTGGAGGCA GATATCTCTG CCCACGAGGA CCGCCTGAAG 
GACCTCAACA GCCAGGCGGA CAGCCTGATG ACCAGCAGCG CCTTCGACAC CTCCCAGGTG 
AAGGACAAGC GGGACGCCAT CAACGGCCGT TTCCAGAAGA TCAAGAGCAT GGCCGCCTCG 
AGGCGAGCTA GGCTGAATGA GTCCCACCGC CTGCACCAGT TTTTCCGGGA CATGGATGAT 
GAGGAGTCCT GGATCAAGGA AAAGAAGCTG CTGGTGAGCT CAGAGGACTA CGGCCGTGAC 
CTGACGGGCG TCCAGAACCT GAGGAAGAAG CACAAACGGC TGGAAGCAGA GCTGGCTGCC 
CACGAGCCGG CCATTCAGGG TGTCCTGGAC ACTGGAAAGA AGCTGTCTGA TGACAACACC 
ATCGGGAAGG AGGAGATTCA GCAGCGCCTG GCGCAGTTCG TGGAGCACTG GAAGGAGCTG 
AAGCAGCTGG CAGCTGCCCG GGGCCAGCGG CTAGAAGAAT CTTTGGAATA TCAGCAGTTT 
GTAGCCAACG TGGAGGAGGA GGAAGCCTGG ATCAATGAGA AGATGACCCT GGTGGCCAGT 
GAAGATTATG GAGACACTCT TGCTGCCATC CAGGGCTTGC TGAAGAAACA CGAAGCCTTC 
GAGACGGACT TCACTGTCCA CAAGGACCGA GTGAATGATG TCTGCACCAA CGGCCAGGAC 
CTGGTTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CCAAGATGAA GGGCTTGAAC 
GGGAAAGTGT CAGACTTGGA GAAGGCTGCC TCCCAGAGGA AGGCCAAGCT CGACGAGAAC 
TCGGCCTTCC TCCAGTTCAA CTGGAAAGCC GATGTGGTGG AGTCCTGGAT CGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGT CGAGATCTGT CTTCTGTCCA AACTCTCCTC 
ACCAAACAGG AAACCTTCGA TGCTGGCCTG CAGGCCTTCC AGCAGGAGGG CATCGCCAAC 
ATCACTGCCC TCAAAGATCA GCTGCTGGCC GCCAAGCACG TTCAGTCCAA GGCCATCGAG 
GCCCGCCATG CTGCCCTCAT GAAGAGGTGG AGCCAGCTCC TGGCCAACTC GGCCACCCGG 
AAGAAGAAGC TGCTGGAGGC ACAGAGTCAC TTCCGCAAGG TGGAGGATCT CTTCCTGACC 
TTTGCTAAAA AGGCTTCAGC CTTCAACAGC TGGTTTGAAA ACGCAGAGGA GGACTTAACC 
GACCCAGTGC GCTGCAACTC TCTGGAAGAG ATCAAGGCCT TGAGAGAGGC CCACGACGCC 
TTCCGCTCCT CGTTGAGCTC GGCCCAGGCC GACTTCAACC AGCTGGCAGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT GGCCTCCAAC CCCTACACCT GGTTCACCAT GGAGGCCCTG 
GAGGAGACCT GGAGAAACCT ACAGAAGATC ATCAAGGAAA GGGAGCTGGA GCTGCAGAAG 
GAGCAGCGAC GGCAGGAGGA GAATGACAAG CTGCGCCAGG AGTTCGCCCA GCATGCCAAC 
GCCTTCCACC AGTGGATCCA GGAGACCAGA ACGTACCTCC TCGATGGCAT AGCGTATCGT 
CGGGTCATTC GTGTCTATCA GTATGAAGTT GGGGATGATC TGTCTGGAAG GTCCTGCATG 
GTGGAAGAAT CCGGGACCCT CGAATCACAG CTTGAAGCTA CCAAACGCAA ACACCAGGAG 
ATTCGAGCTA TGAGAAGTCA GCTCAAGAAG ATTGAAGACC TGGGGGCGGC CATGGAGGAA 
GCGCTGATCC TGGACAACAA GTACACGGAG CACAGCACCG TGGGCCTGGC CCAGCAGTGG 
GACCAGCTCG ACCAGTTGGG CATGCGCATG CAGCACAACC TGGAGCAGCA GATCCAGGCC 
AGGAACACGA CCGGAGTGAC CGAGGAGGCC CTCAAAGAAT TCAGCATGAT GTTCAAACAC 
TTCGACAAGG ACAAGTCTGG CCGGCTGAAC CACCAGGAGT TCAAATCCTG TCTGCGCTCC 
CTGGGCTATG ACCTGCCCAT GGTGGAGGAA GGGGAGCCCG ACCCTGAGTT CGAGGCCATC 
CTGGACACTG TGGATCCTAA CAGGGACGGC CACGTCTCCC TGCAAGAGTA CATGGCCTTT 
ATGATCAGCC GTGAGACCGA GAACGTCAAG TCCAGCGAGG AGATTGAGAG CGCCTTCCGG 
GCCCTGAGCT CTGAAGGGAA GCCTTATGTG ACCAAGGAGG AGCTGTACCA GAACCTGACT 
CGGGAACAAG CCGACTACTG CGTCTCCCAC ATGAAGCCCT ACGTGGACGG CAAGGGCCGC 
GAACTCCCCA CTGCTTTTGA CTACGTCGAG TTCACCCGCT CGCTCTTCGT GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_015473654.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329140.1 Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa37323
Clone ID Related Accession (Same CDS sequence)	XM_015473654.1 , XM_024999035.1 , NM_001114156.1
Accession Version	XM_015473654.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7419bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT 
GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT 
CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC 
AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG 
AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC 
CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG 
AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT 
GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC 
CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC 
CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC 
CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT 
AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT 
GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG 
GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC 
ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC 
TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG 
AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG 
GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA 
GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG 
AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG 
CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG 
GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA 
ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG 
AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT 
GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT 
TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG 
CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT 
GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG 
AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG 
CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT 
CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC 
GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA 
GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG 
GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT 
CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG 
AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC 
AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC 
GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC 
CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC 
GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA 
GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC 
GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC 
AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC 
GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC 
AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGACGC GCGTAACTAA GGAGGCTGGC 
AGCGTGTCTC TGCGTATGAA ACAGGTGGAA GAACTGTACC ATTCTCTGCT GGAACTGGGA 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG 
AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC 
GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC 
AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA 
GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC 
AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG 
CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA 
GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC 
TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG 
GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC 
CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA 
TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC 
CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT 
CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG 
TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC 
GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG 
ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG 
GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG 
ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC 
GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG 
ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA 
GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG 
GACCCCACCA ACATCCAGAG CAAGCATCAG AAGCACCAGG CCTTCGAGGC AGAACTGCAC 
GCCAATGCGG ACCGGATCCG TGGGGTCATC GACATGGGCA ACTCCCTCAT TGACCGCGGG 
GCCTGCGCCG GCAGCGAGGA TGCCGTCAAG GCCCGGCTGG CCGCCTTAGC TGACCAGTGG 
CAGTTCCTGG TGCAGAAGTC GGCTGAGAAG AGCCAGAAGC TGAAAGAAGC CAACAAGCAG 
CAGAACTTCA ACACTGGCAT CAAGGACTTC GACTTCTGGC TCTCTGAGGT GGAAGCCCTG 
CTGGCCTCTG AGGATTATGG CAAAGACCTG GCTTCTGTGA ACAACTTGCT AAAAAAACAT 
CAGCTGCTGG AGGCAGATAT CTCTGCCCAC GAGGACCGCC TGAAGGACCT CAACAGCCAG 
GCGGACAGCC TGATGACCAG CAGCGCCTTC GACACCTCCC AGGTGAAGGA CAAGCGGGAC 
GCCATCAACG GCCGTTTCCA GAAGATCAAG AGCATGGCCG CCTCGAGGCG AGCTAGGCTG 
AATGAGTCCC ACCGCCTGCA CCAGTTTTTC CGGGACATGG ATGATGAGGA GTCCTGGATC 
AAGGAAAAGA AGCTGCTGGT GAGCTCAGAG GACTACGGCC GTGACCTGAC GGGCGTCCAG 
AACCTGAGGA AGAAGCACAA ACGGCTGGAA GCAGAGCTGG CTGCCCACGA GCCGGCCATT 
CAGGGTGTCC TGGACACTGG AAAGAAGCTG TCTGATGACA ACACCATCGG GAAGGAGGAG 
ATTCAGCAGC GCCTGGCGCA GTTCGTGGAG CACTGGAAGG AGCTGAAGCA GCTGGCAGCT 
GCCCGGGGCC AGCGGCTAGA AGAATCTTTG GAATATCAGC AGTTTGTAGC CAACGTGGAG 
GAGGAGGAAG CCTGGATCAA TGAGAAGATG ACCCTGGTGG CCAGTGAAGA TTATGGAGAC 
ACTCTTGCTG CCATCCAGGG CTTGCTGAAG AAACACGAAG CCTTCGAGAC GGACTTCACT 
GTCCACAAGG ACCGAGTGAA TGATGTCTGC ACCAACGGCC AGGACCTGGT TAAGAAGAAC 
AATCACCATG AGGAGAACAT CTCTTCCAAG ATGAAGGGCT TGAACGGGAA AGTGTCAGAC 
TTGGAGAAGG CTGCCTCCCA GAGGAAGGCC AAGCTCGACG AGAACTCGGC CTTCCTCCAG 
TTCAACTGGA AAGCCGATGT GGTGGAGTCC TGGATCGGTG AAAAGGAGAA CAGCTTGAAG 
ACAGATGATT ATGGTCGAGA TCTGTCTTCT GTCCAAACTC TCCTCACCAA ACAGGAAACC 
TTCGATGCTG GCCTGCAGGC CTTCCAGCAG GAGGGCATCG CCAACATCAC TGCCCTCAAA 
GATCAGCTGC TGGCCGCCAA GCACGTTCAG TCCAAGGCCA TCGAGGCCCG CCATGCTGCC 
CTCATGAAGA GGTGGAGCCA GCTCCTGGCC AACTCGGCCA CCCGGAAGAA GAAGCTGCTG 
GAGGCACAGA GTCACTTCCG CAAGGTGGAG GATCTCTTCC TGACCTTTGC TAAAAAGGCT 
TCAGCCTTCA ACAGCTGGTT TGAAAACGCA GAGGAGGACT TAACCGACCC AGTGCGCTGC 
AACTCTCTGG AAGAGATCAA GGCCTTGAGA GAGGCCCACG ACGCCTTCCG CTCCTCGTTG 
AGCTCGGCCC AGGCCGACTT CAACCAGCTG GCAGAGCTGG ACCGCCAGAT CAAGAGCTTC 
CGCGTGGCCT CCAACCCCTA CACCTGGTTC ACCATGGAGG CCCTGGAGGA GACCTGGAGA 
AACCTACAGA AGATCATCAA GGAAAGGGAG CTGGAGCTGC AGAAGGAGCA GCGACGGCAG 
GAGGAGAATG ACAAGCTGCG CCAGGAGTTC GCCCAGCATG CCAACGCCTT CCACCAGTGG 
ATCCAGGAGA CCAGAACGTA CCTCCTCGAT GGGTCCTGCA TGGTGGAAGA ATCCGGGACC 
CTCGAATCAC AGCTTGAAGC TACCAAACGC AAACACCAGG AGATTCGAGC TATGAGAAGT 
CAGCTCAAGA AGATTGAAGA CCTGGGGGCG GCCATGGAGG AAGCGCTGAT CCTGGACAAC 
AAGTACACGG AGCACAGCAC CGTGGGCCTG GCCCAGCAGT GGGACCAGCT CGACCAGTTG 
GGCATGCGCA TGCAGCACAA CCTGGAGCAG CAGATCCAGG CCAGGAACAC GACCGGAGTG 
ACCGAGGAGG CCCTCAAAGA ATTCAGCATG ATGTTCAAAC ACTTCGACAA GGACAAGTCT 
GGCCGGCTGA ACCACCAGGA GTTCAAATCC TGTCTGCGCT CCCTGGGCTA TGACCTGCCC 
ATGGTGGAGG AAGGGGAGCC CGACCCTGAG TTCGAGGCCA TCCTGGACAC TGTGGATCCT 
AACAGGGACG GCCACGTCTC CCTGCAAGAG TACATGGCCT TTATGATCAG CCGTGAGACC 
GAGAACGTCA AGTCCAGCGA GGAGATTGAG AGCGCCTTCC GGGCCCTGAG CTCTGAAGGG 
AAGCCTTATG TGACCAAGGA GGAGCTGTAC CAGAACCTGA CTCGGGAACA AGCCGACTAC 
TGCGTCTCCC ACATGAAGCC CTACGTGGAC GGCAAGGGCC GCGAACTCCC CACTGCTTTT 
GACTACGTCG AGTTCACCCG CTCGCTCTTC GTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329140.1
CDS	26..7444
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLHQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMYRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVDHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWESLKSKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRVTKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGAMPRDESDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKEKLDVLDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQSFADQLIAGGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQSKHQKHQAFEAELHANADRIRGVIDMGNSLIDRGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDAINGRFQKIKSMAASRRARLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLVKKNNHHEENISSKMKGLNGKVSDLEKAASQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHAALMKRWSQLLANSATRKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_015473654.1
Organism	Bos taurus(cattle)
Definition	Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473654.1

ORF Insert Sequence:

ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT 
GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT 
CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC 
AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG 
AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC 
CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG 
AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT 
GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC 
CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC 
CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC 
CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT 
AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT 
GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG 
GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC 
ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC 
TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG 
AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG 
GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA 
GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG 
AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG 
CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG 
GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA 
ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG 
AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT 
GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT 
TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG 
CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT 
GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG 
AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG 
CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT 
CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC 
GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA 
GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG 
GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT 
CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG 
AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC 
AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC 
GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC 
CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC 
GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA 
GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC 
GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC 
AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC 
GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC 
AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGACGC GCGTAACTAA GGAGGCTGGC 
AGCGTGTCTC TGCGTATGAA ACAGGTGGAA GAACTGTACC ATTCTCTGCT GGAACTGGGA 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG 
AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC 
GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC 
AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA 
GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC 
AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG 
CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA 
GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC 
TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG 
GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC 
CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA 
TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC 
CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT 
CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG 
TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC 
GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG 
ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG 
GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG 
ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC 
GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG 
ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA 
GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG 
GACCCCACCA ACATCCAGAG CAAGCATCAG AAGCACCAGG CCTTCGAGGC AGAACTGCAC 
GCCAATGCGG ACCGGATCCG TGGGGTCATC GACATGGGCA ACTCCCTCAT TGACCGCGGG 
GCCTGCGCCG GCAGCGAGGA TGCCGTCAAG GCCCGGCTGG CCGCCTTAGC TGACCAGTGG 
CAGTTCCTGG TGCAGAAGTC GGCTGAGAAG AGCCAGAAGC TGAAAGAAGC CAACAAGCAG 
CAGAACTTCA ACACTGGCAT CAAGGACTTC GACTTCTGGC TCTCTGAGGT GGAAGCCCTG 
CTGGCCTCTG AGGATTATGG CAAAGACCTG GCTTCTGTGA ACAACTTGCT AAAAAAACAT 
CAGCTGCTGG AGGCAGATAT CTCTGCCCAC GAGGACCGCC TGAAGGACCT CAACAGCCAG 
GCGGACAGCC TGATGACCAG CAGCGCCTTC GACACCTCCC AGGTGAAGGA CAAGCGGGAC 
GCCATCAACG GCCGTTTCCA GAAGATCAAG AGCATGGCCG CCTCGAGGCG AGCTAGGCTG 
AATGAGTCCC ACCGCCTGCA CCAGTTTTTC CGGGACATGG ATGATGAGGA GTCCTGGATC 
AAGGAAAAGA AGCTGCTGGT GAGCTCAGAG GACTACGGCC GTGACCTGAC GGGCGTCCAG 
AACCTGAGGA AGAAGCACAA ACGGCTGGAA GCAGAGCTGG CTGCCCACGA GCCGGCCATT 
CAGGGTGTCC TGGACACTGG AAAGAAGCTG TCTGATGACA ACACCATCGG GAAGGAGGAG 
ATTCAGCAGC GCCTGGCGCA GTTCGTGGAG CACTGGAAGG AGCTGAAGCA GCTGGCAGCT 
GCCCGGGGCC AGCGGCTAGA AGAATCTTTG GAATATCAGC AGTTTGTAGC CAACGTGGAG 
GAGGAGGAAG CCTGGATCAA TGAGAAGATG ACCCTGGTGG CCAGTGAAGA TTATGGAGAC 
ACTCTTGCTG CCATCCAGGG CTTGCTGAAG AAACACGAAG CCTTCGAGAC GGACTTCACT 
GTCCACAAGG ACCGAGTGAA TGATGTCTGC ACCAACGGCC AGGACCTGGT TAAGAAGAAC 
AATCACCATG AGGAGAACAT CTCTTCCAAG ATGAAGGGCT TGAACGGGAA AGTGTCAGAC 
TTGGAGAAGG CTGCCTCCCA GAGGAAGGCC AAGCTCGACG AGAACTCGGC CTTCCTCCAG 
TTCAACTGGA AAGCCGATGT GGTGGAGTCC TGGATCGGTG AAAAGGAGAA CAGCTTGAAG 
ACAGATGATT ATGGTCGAGA TCTGTCTTCT GTCCAAACTC TCCTCACCAA ACAGGAAACC 
TTCGATGCTG GCCTGCAGGC CTTCCAGCAG GAGGGCATCG CCAACATCAC TGCCCTCAAA 
GATCAGCTGC TGGCCGCCAA GCACGTTCAG TCCAAGGCCA TCGAGGCCCG CCATGCTGCC 
CTCATGAAGA GGTGGAGCCA GCTCCTGGCC AACTCGGCCA CCCGGAAGAA GAAGCTGCTG 
GAGGCACAGA GTCACTTCCG CAAGGTGGAG GATCTCTTCC TGACCTTTGC TAAAAAGGCT 
TCAGCCTTCA ACAGCTGGTT TGAAAACGCA GAGGAGGACT TAACCGACCC AGTGCGCTGC 
AACTCTCTGG AAGAGATCAA GGCCTTGAGA GAGGCCCACG ACGCCTTCCG CTCCTCGTTG 
AGCTCGGCCC AGGCCGACTT CAACCAGCTG GCAGAGCTGG ACCGCCAGAT CAAGAGCTTC 
CGCGTGGCCT CCAACCCCTA CACCTGGTTC ACCATGGAGG CCCTGGAGGA GACCTGGAGA 
AACCTACAGA AGATCATCAA GGAAAGGGAG CTGGAGCTGC AGAAGGAGCA GCGACGGCAG 
GAGGAGAATG ACAAGCTGCG CCAGGAGTTC GCCCAGCATG CCAACGCCTT CCACCAGTGG 
ATCCAGGAGA CCAGAACGTA CCTCCTCGAT GGGTCCTGCA TGGTGGAAGA ATCCGGGACC 
CTCGAATCAC AGCTTGAAGC TACCAAACGC AAACACCAGG AGATTCGAGC TATGAGAAGT 
CAGCTCAAGA AGATTGAAGA CCTGGGGGCG GCCATGGAGG AAGCGCTGAT CCTGGACAAC 
AAGTACACGG AGCACAGCAC CGTGGGCCTG GCCCAGCAGT GGGACCAGCT CGACCAGTTG 
GGCATGCGCA TGCAGCACAA CCTGGAGCAG CAGATCCAGG CCAGGAACAC GACCGGAGTG 
ACCGAGGAGG CCCTCAAAGA ATTCAGCATG ATGTTCAAAC ACTTCGACAA GGACAAGTCT 
GGCCGGCTGA ACCACCAGGA GTTCAAATCC TGTCTGCGCT CCCTGGGCTA TGACCTGCCC 
ATGGTGGAGG AAGGGGAGCC CGACCCTGAG TTCGAGGCCA TCCTGGACAC TGTGGATCCT 
AACAGGGACG GCCACGTCTC CCTGCAAGAG TACATGGCCT TTATGATCAG CCGTGAGACC 
GAGAACGTCA AGTCCAGCGA GGAGATTGAG AGCGCCTTCC GGGCCCTGAG CTCTGAAGGG 
AAGCCTTATG TGACCAAGGA GGAGCTGTAC CAGAACCTGA CTCGGGAACA AGCCGACTAC 
TGCGTCTCCC ACATGAAGCC CTACGTGGAC GGCAAGGGCC GCGAACTCCC CACTGCTTTT 
GACTACGTCG AGTTCACCCG CTCGCTCTTC GTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_024999035.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854803.1 Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OBa37323
Clone ID Related Accession (Same CDS sequence)	XM_015473654.1 , XM_024999035.1 , NM_001114156.1
Accession Version	XM_024999035.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7419bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT 
GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT 
CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC 
AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG 
AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC 
CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG 
AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT 
GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC 
CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC 
CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC 
CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT 
AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT 
GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG 
GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC 
ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC 
TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG 
AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG 
GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA 
GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG 
AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG 
CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG 
GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA 
ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG 
AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT 
GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT 
TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG 
CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT 
GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG 
AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG 
CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT 
CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC 
GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA 
GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG 
GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT 
CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG 
AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC 
AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC 
GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC 
CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC 
GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA 
GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC 
GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC 
AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC 
GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC 
AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGACGC GCGTAACTAA GGAGGCTGGC 
AGCGTGTCTC TGCGTATGAA ACAGGTGGAA GAACTGTACC ATTCTCTGCT GGAACTGGGA 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG 
AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC 
GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC 
AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA 
GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC 
AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG 
CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA 
GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC 
TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG 
GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC 
CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA 
TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC 
CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT 
CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG 
TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC 
GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG 
ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG 
GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG 
ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC 
GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG 
ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA 
GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG 
GACCCCACCA ACATCCAGAG CAAGCATCAG AAGCACCAGG CCTTCGAGGC AGAACTGCAC 
GCCAATGCGG ACCGGATCCG TGGGGTCATC GACATGGGCA ACTCCCTCAT TGACCGCGGG 
GCCTGCGCCG GCAGCGAGGA TGCCGTCAAG GCCCGGCTGG CCGCCTTAGC TGACCAGTGG 
CAGTTCCTGG TGCAGAAGTC GGCTGAGAAG AGCCAGAAGC TGAAAGAAGC CAACAAGCAG 
CAGAACTTCA ACACTGGCAT CAAGGACTTC GACTTCTGGC TCTCTGAGGT GGAAGCCCTG 
CTGGCCTCTG AGGATTATGG CAAAGACCTG GCTTCTGTGA ACAACTTGCT AAAAAAACAT 
CAGCTGCTGG AGGCAGATAT CTCTGCCCAC GAGGACCGCC TGAAGGACCT CAACAGCCAG 
GCGGACAGCC TGATGACCAG CAGCGCCTTC GACACCTCCC AGGTGAAGGA CAAGCGGGAC 
GCCATCAACG GCCGTTTCCA GAAGATCAAG AGCATGGCCG CCTCGAGGCG AGCTAGGCTG 
AATGAGTCCC ACCGCCTGCA CCAGTTTTTC CGGGACATGG ATGATGAGGA GTCCTGGATC 
AAGGAAAAGA AGCTGCTGGT GAGCTCAGAG GACTACGGCC GTGACCTGAC GGGCGTCCAG 
AACCTGAGGA AGAAGCACAA ACGGCTGGAA GCAGAGCTGG CTGCCCACGA GCCGGCCATT 
CAGGGTGTCC TGGACACTGG AAAGAAGCTG TCTGATGACA ACACCATCGG GAAGGAGGAG 
ATTCAGCAGC GCCTGGCGCA GTTCGTGGAG CACTGGAAGG AGCTGAAGCA GCTGGCAGCT 
GCCCGGGGCC AGCGGCTAGA AGAATCTTTG GAATATCAGC AGTTTGTAGC CAACGTGGAG 
GAGGAGGAAG CCTGGATCAA TGAGAAGATG ACCCTGGTGG CCAGTGAAGA TTATGGAGAC 
ACTCTTGCTG CCATCCAGGG CTTGCTGAAG AAACACGAAG CCTTCGAGAC GGACTTCACT 
GTCCACAAGG ACCGAGTGAA TGATGTCTGC ACCAACGGCC AGGACCTGGT TAAGAAGAAC 
AATCACCATG AGGAGAACAT CTCTTCCAAG ATGAAGGGCT TGAACGGGAA AGTGTCAGAC 
TTGGAGAAGG CTGCCTCCCA GAGGAAGGCC AAGCTCGACG AGAACTCGGC CTTCCTCCAG 
TTCAACTGGA AAGCCGATGT GGTGGAGTCC TGGATCGGTG AAAAGGAGAA CAGCTTGAAG 
ACAGATGATT ATGGTCGAGA TCTGTCTTCT GTCCAAACTC TCCTCACCAA ACAGGAAACC 
TTCGATGCTG GCCTGCAGGC CTTCCAGCAG GAGGGCATCG CCAACATCAC TGCCCTCAAA 
GATCAGCTGC TGGCCGCCAA GCACGTTCAG TCCAAGGCCA TCGAGGCCCG CCATGCTGCC 
CTCATGAAGA GGTGGAGCCA GCTCCTGGCC AACTCGGCCA CCCGGAAGAA GAAGCTGCTG 
GAGGCACAGA GTCACTTCCG CAAGGTGGAG GATCTCTTCC TGACCTTTGC TAAAAAGGCT 
TCAGCCTTCA ACAGCTGGTT TGAAAACGCA GAGGAGGACT TAACCGACCC AGTGCGCTGC 
AACTCTCTGG AAGAGATCAA GGCCTTGAGA GAGGCCCACG ACGCCTTCCG CTCCTCGTTG 
AGCTCGGCCC AGGCCGACTT CAACCAGCTG GCAGAGCTGG ACCGCCAGAT CAAGAGCTTC 
CGCGTGGCCT CCAACCCCTA CACCTGGTTC ACCATGGAGG CCCTGGAGGA GACCTGGAGA 
AACCTACAGA AGATCATCAA GGAAAGGGAG CTGGAGCTGC AGAAGGAGCA GCGACGGCAG 
GAGGAGAATG ACAAGCTGCG CCAGGAGTTC GCCCAGCATG CCAACGCCTT CCACCAGTGG 
ATCCAGGAGA CCAGAACGTA CCTCCTCGAT GGGTCCTGCA TGGTGGAAGA ATCCGGGACC 
CTCGAATCAC AGCTTGAAGC TACCAAACGC AAACACCAGG AGATTCGAGC TATGAGAAGT 
CAGCTCAAGA AGATTGAAGA CCTGGGGGCG GCCATGGAGG AAGCGCTGAT CCTGGACAAC 
AAGTACACGG AGCACAGCAC CGTGGGCCTG GCCCAGCAGT GGGACCAGCT CGACCAGTTG 
GGCATGCGCA TGCAGCACAA CCTGGAGCAG CAGATCCAGG CCAGGAACAC GACCGGAGTG 
ACCGAGGAGG CCCTCAAAGA ATTCAGCATG ATGTTCAAAC ACTTCGACAA GGACAAGTCT 
GGCCGGCTGA ACCACCAGGA GTTCAAATCC TGTCTGCGCT CCCTGGGCTA TGACCTGCCC 
ATGGTGGAGG AAGGGGAGCC CGACCCTGAG TTCGAGGCCA TCCTGGACAC TGTGGATCCT 
AACAGGGACG GCCACGTCTC CCTGCAAGAG TACATGGCCT TTATGATCAG CCGTGAGACC 
GAGAACGTCA AGTCCAGCGA GGAGATTGAG AGCGCCTTCC GGGCCCTGAG CTCTGAAGGG 
AAGCCTTATG TGACCAAGGA GGAGCTGTAC CAGAACCTGA CTCGGGAACA AGCCGACTAC 
TGCGTCTCCC ACATGAAGCC CTACGTGGAC GGCAAGGGCC GCGAACTCCC CACTGCTTTT 
GACTACGTCG AGTTCACCCG CTCGCTCTTC GTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854803.1
CDS	120..7538
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLHQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMYRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVDHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWESLKSKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRVTKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGAMPRDESDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKEKLDVLDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQSFADQLIAGGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQSKHQKHQAFEAELHANADRIRGVIDMGNSLIDRGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDAINGRFQKIKSMAASRRARLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLVKKNNHHEENISSKMKGLNGKVSDLEKAASQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHAALMKRWSQLLANSATRKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_024999035.1
Organism	Bos taurus(cattle)
Definition	Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999035.1

ORF Insert Sequence:

ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT 
GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT 
CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC 
AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG 
AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC 
CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG 
AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT 
GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC 
CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC 
CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC 
CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT 
AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT 
GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG 
GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC 
ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC 
TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG 
AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG 
GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA 
GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG 
AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG 
CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG 
GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA 
ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG 
AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT 
GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT 
TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG 
CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT 
GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG 
AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG 
CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT 
CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC 
GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA 
GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG 
GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT 
CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG 
AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC 
AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC 
GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC 
CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC 
GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA 
GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC 
GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC 
AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC 
GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC 
AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGACGC GCGTAACTAA GGAGGCTGGC 
AGCGTGTCTC TGCGTATGAA ACAGGTGGAA GAACTGTACC ATTCTCTGCT GGAACTGGGA 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG 
AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC 
GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC 
AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA 
GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC 
AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG 
CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA 
GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC 
TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG 
GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC 
CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA 
TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC 
CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT 
CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG 
TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC 
GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG 
ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG 
GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG 
ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC 
GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG 
ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA 
GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG 
GACCCCACCA ACATCCAGAG CAAGCATCAG AAGCACCAGG CCTTCGAGGC AGAACTGCAC 
GCCAATGCGG ACCGGATCCG TGGGGTCATC GACATGGGCA ACTCCCTCAT TGACCGCGGG 
GCCTGCGCCG GCAGCGAGGA TGCCGTCAAG GCCCGGCTGG CCGCCTTAGC TGACCAGTGG 
CAGTTCCTGG TGCAGAAGTC GGCTGAGAAG AGCCAGAAGC TGAAAGAAGC CAACAAGCAG 
CAGAACTTCA ACACTGGCAT CAAGGACTTC GACTTCTGGC TCTCTGAGGT GGAAGCCCTG 
CTGGCCTCTG AGGATTATGG CAAAGACCTG GCTTCTGTGA ACAACTTGCT AAAAAAACAT 
CAGCTGCTGG AGGCAGATAT CTCTGCCCAC GAGGACCGCC TGAAGGACCT CAACAGCCAG 
GCGGACAGCC TGATGACCAG CAGCGCCTTC GACACCTCCC AGGTGAAGGA CAAGCGGGAC 
GCCATCAACG GCCGTTTCCA GAAGATCAAG AGCATGGCCG CCTCGAGGCG AGCTAGGCTG 
AATGAGTCCC ACCGCCTGCA CCAGTTTTTC CGGGACATGG ATGATGAGGA GTCCTGGATC 
AAGGAAAAGA AGCTGCTGGT GAGCTCAGAG GACTACGGCC GTGACCTGAC GGGCGTCCAG 
AACCTGAGGA AGAAGCACAA ACGGCTGGAA GCAGAGCTGG CTGCCCACGA GCCGGCCATT 
CAGGGTGTCC TGGACACTGG AAAGAAGCTG TCTGATGACA ACACCATCGG GAAGGAGGAG 
ATTCAGCAGC GCCTGGCGCA GTTCGTGGAG CACTGGAAGG AGCTGAAGCA GCTGGCAGCT 
GCCCGGGGCC AGCGGCTAGA AGAATCTTTG GAATATCAGC AGTTTGTAGC CAACGTGGAG 
GAGGAGGAAG CCTGGATCAA TGAGAAGATG ACCCTGGTGG CCAGTGAAGA TTATGGAGAC 
ACTCTTGCTG CCATCCAGGG CTTGCTGAAG AAACACGAAG CCTTCGAGAC GGACTTCACT 
GTCCACAAGG ACCGAGTGAA TGATGTCTGC ACCAACGGCC AGGACCTGGT TAAGAAGAAC 
AATCACCATG AGGAGAACAT CTCTTCCAAG ATGAAGGGCT TGAACGGGAA AGTGTCAGAC 
TTGGAGAAGG CTGCCTCCCA GAGGAAGGCC AAGCTCGACG AGAACTCGGC CTTCCTCCAG 
TTCAACTGGA AAGCCGATGT GGTGGAGTCC TGGATCGGTG AAAAGGAGAA CAGCTTGAAG 
ACAGATGATT ATGGTCGAGA TCTGTCTTCT GTCCAAACTC TCCTCACCAA ACAGGAAACC 
TTCGATGCTG GCCTGCAGGC CTTCCAGCAG GAGGGCATCG CCAACATCAC TGCCCTCAAA 
GATCAGCTGC TGGCCGCCAA GCACGTTCAG TCCAAGGCCA TCGAGGCCCG CCATGCTGCC 
CTCATGAAGA GGTGGAGCCA GCTCCTGGCC AACTCGGCCA CCCGGAAGAA GAAGCTGCTG 
GAGGCACAGA GTCACTTCCG CAAGGTGGAG GATCTCTTCC TGACCTTTGC TAAAAAGGCT 
TCAGCCTTCA ACAGCTGGTT TGAAAACGCA GAGGAGGACT TAACCGACCC AGTGCGCTGC 
AACTCTCTGG AAGAGATCAA GGCCTTGAGA GAGGCCCACG ACGCCTTCCG CTCCTCGTTG 
AGCTCGGCCC AGGCCGACTT CAACCAGCTG GCAGAGCTGG ACCGCCAGAT CAAGAGCTTC 
CGCGTGGCCT CCAACCCCTA CACCTGGTTC ACCATGGAGG CCCTGGAGGA GACCTGGAGA 
AACCTACAGA AGATCATCAA GGAAAGGGAG CTGGAGCTGC AGAAGGAGCA GCGACGGCAG 
GAGGAGAATG ACAAGCTGCG CCAGGAGTTC GCCCAGCATG CCAACGCCTT CCACCAGTGG 
ATCCAGGAGA CCAGAACGTA CCTCCTCGAT GGGTCCTGCA TGGTGGAAGA ATCCGGGACC 
CTCGAATCAC AGCTTGAAGC TACCAAACGC AAACACCAGG AGATTCGAGC TATGAGAAGT 
CAGCTCAAGA AGATTGAAGA CCTGGGGGCG GCCATGGAGG AAGCGCTGAT CCTGGACAAC 
AAGTACACGG AGCACAGCAC CGTGGGCCTG GCCCAGCAGT GGGACCAGCT CGACCAGTTG 
GGCATGCGCA TGCAGCACAA CCTGGAGCAG CAGATCCAGG CCAGGAACAC GACCGGAGTG 
ACCGAGGAGG CCCTCAAAGA ATTCAGCATG ATGTTCAAAC ACTTCGACAA GGACAAGTCT 
GGCCGGCTGA ACCACCAGGA GTTCAAATCC TGTCTGCGCT CCCTGGGCTA TGACCTGCCC 
ATGGTGGAGG AAGGGGAGCC CGACCCTGAG TTCGAGGCCA TCCTGGACAC TGTGGATCCT 
AACAGGGACG GCCACGTCTC CCTGCAAGAG TACATGGCCT TTATGATCAG CCGTGAGACC 
GAGAACGTCA AGTCCAGCGA GGAGATTGAG AGCGCCTTCC GGGCCCTGAG CTCTGAAGGG 
AAGCCTTATG TGACCAAGGA GGAGCTGTAC CAGAACCTGA CTCGGGAACA AGCCGACTAC 
TGCGTCTCCC ACATGAAGCC CTACGTGGAC GGCAAGGGCC GCGAACTCCC CACTGCTTTT 
GACTACGTCG AGTTCACCCG CTCGCTCTTC GTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_010810459.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010808761.1 Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa54551
Clone ID Related Accession (Same CDS sequence)	XM_010810459.2 , XM_024999033.1
Accession Version	XM_010810459.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7434bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810459.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT 
GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT 
CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC 
AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG 
AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC 
CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG 
AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT 
GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC 
CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC 
CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC 
CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT 
AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT 
GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG 
GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC 
ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC 
TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG 
AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG 
GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA 
GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG 
AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG 
CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG 
GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA 
ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG 
AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT 
GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT 
TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG 
CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT 
GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG 
AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG 
CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT 
CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC 
GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA 
GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG 
GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT 
CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG 
AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC 
AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC 
GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC 
CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC 
GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA 
GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC 
GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC 
AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC 
GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC 
AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGACGC GCGTAACTAA GGAGGCTGGC 
AGCGTGTCTC TGCGTATGAA ACAGGTGGAA GAACTGTACC ATTCTCTGCT GGAACTGGGA 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG 
AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC 
GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC 
AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA 
GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC 
AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG 
CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA 
GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC 
TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG 
GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC 
CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA 
TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC 
CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT 
CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG 
TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC 
GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG 
ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG 
GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG 
ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC 
GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG 
ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA 
GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG 
GACCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ATCAGAAGCA CCAGGCCTTC 
GAGGCAGAAC TGCACGCCAA TGCGGACCGG ATCCGTGGGG TCATCGACAT GGGCAACTCC 
CTCATTGACC GCGGGGCCTG CGCCGGCAGC GAGGATGCCG TCAAGGCCCG GCTGGCCGCC 
TTAGCTGACC AGTGGCAGTT CCTGGTGCAG AAGTCGGCTG AGAAGAGCCA GAAGCTGAAA 
GAAGCCAACA AGCAGCAGAA CTTCAACACT GGCATCAAGG ACTTCGACTT CTGGCTCTCT 
GAGGTGGAAG CCCTGCTGGC CTCTGAGGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC 
TTGCTAAAAA AACATCAGCT GCTGGAGGCA GATATCTCTG CCCACGAGGA CCGCCTGAAG 
GACCTCAACA GCCAGGCGGA CAGCCTGATG ACCAGCAGCG CCTTCGACAC CTCCCAGGTG 
AAGGACAAGC GGGACGCCAT CAACGGCCGT TTCCAGAAGA TCAAGAGCAT GGCCGCCTCG 
AGGCGAGCTA GGCTGAATGA GTCCCACCGC CTGCACCAGT TTTTCCGGGA CATGGATGAT 
GAGGAGTCCT GGATCAAGGA AAAGAAGCTG CTGGTGAGCT CAGAGGACTA CGGCCGTGAC 
CTGACGGGCG TCCAGAACCT GAGGAAGAAG CACAAACGGC TGGAAGCAGA GCTGGCTGCC 
CACGAGCCGG CCATTCAGGG TGTCCTGGAC ACTGGAAAGA AGCTGTCTGA TGACAACACC 
ATCGGGAAGG AGGAGATTCA GCAGCGCCTG GCGCAGTTCG TGGAGCACTG GAAGGAGCTG 
AAGCAGCTGG CAGCTGCCCG GGGCCAGCGG CTAGAAGAAT CTTTGGAATA TCAGCAGTTT 
GTAGCCAACG TGGAGGAGGA GGAAGCCTGG ATCAATGAGA AGATGACCCT GGTGGCCAGT 
GAAGATTATG GAGACACTCT TGCTGCCATC CAGGGCTTGC TGAAGAAACA CGAAGCCTTC 
GAGACGGACT TCACTGTCCA CAAGGACCGA GTGAATGATG TCTGCACCAA CGGCCAGGAC 
CTGGTTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CCAAGATGAA GGGCTTGAAC 
GGGAAAGTGT CAGACTTGGA GAAGGCTGCC TCCCAGAGGA AGGCCAAGCT CGACGAGAAC 
TCGGCCTTCC TCCAGTTCAA CTGGAAAGCC GATGTGGTGG AGTCCTGGAT CGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGT CGAGATCTGT CTTCTGTCCA AACTCTCCTC 
ACCAAACAGG AAACCTTCGA TGCTGGCCTG CAGGCCTTCC AGCAGGAGGG CATCGCCAAC 
ATCACTGCCC TCAAAGATCA GCTGCTGGCC GCCAAGCACG TTCAGTCCAA GGCCATCGAG 
GCCCGCCATG CTGCCCTCAT GAAGAGGTGG AGCCAGCTCC TGGCCAACTC GGCCACCCGG 
AAGAAGAAGC TGCTGGAGGC ACAGAGTCAC TTCCGCAAGG TGGAGGATCT CTTCCTGACC 
TTTGCTAAAA AGGCTTCAGC CTTCAACAGC TGGTTTGAAA ACGCAGAGGA GGACTTAACC 
GACCCAGTGC GCTGCAACTC TCTGGAAGAG ATCAAGGCCT TGAGAGAGGC CCACGACGCC 
TTCCGCTCCT CGTTGAGCTC GGCCCAGGCC GACTTCAACC AGCTGGCAGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT GGCCTCCAAC CCCTACACCT GGTTCACCAT GGAGGCCCTG 
GAGGAGACCT GGAGAAACCT ACAGAAGATC ATCAAGGAAA GGGAGCTGGA GCTGCAGAAG 
GAGCAGCGAC GGCAGGAGGA GAATGACAAG CTGCGCCAGG AGTTCGCCCA GCATGCCAAC 
GCCTTCCACC AGTGGATCCA GGAGACCAGA ACGTACCTCC TCGATGGGTC CTGCATGGTG 
GAAGAATCCG GGACCCTCGA ATCACAGCTT GAAGCTACCA AACGCAAACA CCAGGAGATT 
CGAGCTATGA GAAGTCAGCT CAAGAAGATT GAAGACCTGG GGGCGGCCAT GGAGGAAGCG 
CTGATCCTGG ACAACAAGTA CACGGAGCAC AGCACCGTGG GCCTGGCCCA GCAGTGGGAC 
CAGCTCGACC AGTTGGGCAT GCGCATGCAG CACAACCTGG AGCAGCAGAT CCAGGCCAGG 
AACACGACCG GAGTGACCGA GGAGGCCCTC AAAGAATTCA GCATGATGTT CAAACACTTC 
GACAAGGACA AGTCTGGCCG GCTGAACCAC CAGGAGTTCA AATCCTGTCT GCGCTCCCTG 
GGCTATGACC TGCCCATGGT GGAGGAAGGG GAGCCCGACC CTGAGTTCGA GGCCATCCTG 
GACACTGTGG ATCCTAACAG GGACGGCCAC GTCTCCCTGC AAGAGTACAT GGCCTTTATG 
ATCAGCCGTG AGACCGAGAA CGTCAAGTCC AGCGAGGAGA TTGAGAGCGC CTTCCGGGCC 
CTGAGCTCTG AAGGGAAGCC TTATGTGACC AAGGAGGAGC TGTACCAGAA CCTGACTCGG 
GAACAAGCCG ACTACTGCGT CTCCCACATG AAGCCCTACG TGGACGGCAA GGGCCGCGAA 
CTCCCCACTG CTTTTGACTA CGTCGAGTTC ACCCGCTCGC TCTTCGTGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010808761.1
CDS	26..7459
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLHQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMYRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVDHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWESLKSKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRVTKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGAMPRDESDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKEKLDVLDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQSFADQLIAGGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDMGNSLIDRGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDAINGRFQKIKSMAASRRARLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLVKKNNHHEENISSKMKGLNGKVSDLEKAASQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHAALMKRWSQLLANSATRKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_010810459.2
Organism	Bos taurus(cattle)
Definition	Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010810459.2

ORF Insert Sequence:

ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT 
GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT 
CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC 
AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG 
AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC 
CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG 
AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT 
GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC 
CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC 
CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC 
CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT 
AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT 
GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG 
GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC 
ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC 
TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG 
AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG 
GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA 
GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG 
AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG 
CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG 
GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA 
ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG 
AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT 
GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT 
TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG 
CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT 
GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG 
AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG 
CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT 
CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC 
GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA 
GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG 
GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT 
CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG 
AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC 
AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC 
GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC 
CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC 
GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA 
GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC 
GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC 
AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC 
GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC 
AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGACGC GCGTAACTAA GGAGGCTGGC 
AGCGTGTCTC TGCGTATGAA ACAGGTGGAA GAACTGTACC ATTCTCTGCT GGAACTGGGA 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG 
AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC 
GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC 
AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA 
GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC 
AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG 
CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA 
GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC 
TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG 
GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC 
CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA 
TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC 
CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT 
CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG 
TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC 
GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG 
ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG 
GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG 
ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC 
GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG 
ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA 
GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG 
GACCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ATCAGAAGCA CCAGGCCTTC 
GAGGCAGAAC TGCACGCCAA TGCGGACCGG ATCCGTGGGG TCATCGACAT GGGCAACTCC 
CTCATTGACC GCGGGGCCTG CGCCGGCAGC GAGGATGCCG TCAAGGCCCG GCTGGCCGCC 
TTAGCTGACC AGTGGCAGTT CCTGGTGCAG AAGTCGGCTG AGAAGAGCCA GAAGCTGAAA 
GAAGCCAACA AGCAGCAGAA CTTCAACACT GGCATCAAGG ACTTCGACTT CTGGCTCTCT 
GAGGTGGAAG CCCTGCTGGC CTCTGAGGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC 
TTGCTAAAAA AACATCAGCT GCTGGAGGCA GATATCTCTG CCCACGAGGA CCGCCTGAAG 
GACCTCAACA GCCAGGCGGA CAGCCTGATG ACCAGCAGCG CCTTCGACAC CTCCCAGGTG 
AAGGACAAGC GGGACGCCAT CAACGGCCGT TTCCAGAAGA TCAAGAGCAT GGCCGCCTCG 
AGGCGAGCTA GGCTGAATGA GTCCCACCGC CTGCACCAGT TTTTCCGGGA CATGGATGAT 
GAGGAGTCCT GGATCAAGGA AAAGAAGCTG CTGGTGAGCT CAGAGGACTA CGGCCGTGAC 
CTGACGGGCG TCCAGAACCT GAGGAAGAAG CACAAACGGC TGGAAGCAGA GCTGGCTGCC 
CACGAGCCGG CCATTCAGGG TGTCCTGGAC ACTGGAAAGA AGCTGTCTGA TGACAACACC 
ATCGGGAAGG AGGAGATTCA GCAGCGCCTG GCGCAGTTCG TGGAGCACTG GAAGGAGCTG 
AAGCAGCTGG CAGCTGCCCG GGGCCAGCGG CTAGAAGAAT CTTTGGAATA TCAGCAGTTT 
GTAGCCAACG TGGAGGAGGA GGAAGCCTGG ATCAATGAGA AGATGACCCT GGTGGCCAGT 
GAAGATTATG GAGACACTCT TGCTGCCATC CAGGGCTTGC TGAAGAAACA CGAAGCCTTC 
GAGACGGACT TCACTGTCCA CAAGGACCGA GTGAATGATG TCTGCACCAA CGGCCAGGAC 
CTGGTTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CCAAGATGAA GGGCTTGAAC 
GGGAAAGTGT CAGACTTGGA GAAGGCTGCC TCCCAGAGGA AGGCCAAGCT CGACGAGAAC 
TCGGCCTTCC TCCAGTTCAA CTGGAAAGCC GATGTGGTGG AGTCCTGGAT CGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGT CGAGATCTGT CTTCTGTCCA AACTCTCCTC 
ACCAAACAGG AAACCTTCGA TGCTGGCCTG CAGGCCTTCC AGCAGGAGGG CATCGCCAAC 
ATCACTGCCC TCAAAGATCA GCTGCTGGCC GCCAAGCACG TTCAGTCCAA GGCCATCGAG 
GCCCGCCATG CTGCCCTCAT GAAGAGGTGG AGCCAGCTCC TGGCCAACTC GGCCACCCGG 
AAGAAGAAGC TGCTGGAGGC ACAGAGTCAC TTCCGCAAGG TGGAGGATCT CTTCCTGACC 
TTTGCTAAAA AGGCTTCAGC CTTCAACAGC TGGTTTGAAA ACGCAGAGGA GGACTTAACC 
GACCCAGTGC GCTGCAACTC TCTGGAAGAG ATCAAGGCCT TGAGAGAGGC CCACGACGCC 
TTCCGCTCCT CGTTGAGCTC GGCCCAGGCC GACTTCAACC AGCTGGCAGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT GGCCTCCAAC CCCTACACCT GGTTCACCAT GGAGGCCCTG 
GAGGAGACCT GGAGAAACCT ACAGAAGATC ATCAAGGAAA GGGAGCTGGA GCTGCAGAAG 
GAGCAGCGAC GGCAGGAGGA GAATGACAAG CTGCGCCAGG AGTTCGCCCA GCATGCCAAC 
GCCTTCCACC AGTGGATCCA GGAGACCAGA ACGTACCTCC TCGATGGGTC CTGCATGGTG 
GAAGAATCCG GGACCCTCGA ATCACAGCTT GAAGCTACCA AACGCAAACA CCAGGAGATT 
CGAGCTATGA GAAGTCAGCT CAAGAAGATT GAAGACCTGG GGGCGGCCAT GGAGGAAGCG 
CTGATCCTGG ACAACAAGTA CACGGAGCAC AGCACCGTGG GCCTGGCCCA GCAGTGGGAC 
CAGCTCGACC AGTTGGGCAT GCGCATGCAG CACAACCTGG AGCAGCAGAT CCAGGCCAGG 
AACACGACCG GAGTGACCGA GGAGGCCCTC AAAGAATTCA GCATGATGTT CAAACACTTC 
GACAAGGACA AGTCTGGCCG GCTGAACCAC CAGGAGTTCA AATCCTGTCT GCGCTCCCTG 
GGCTATGACC TGCCCATGGT GGAGGAAGGG GAGCCCGACC CTGAGTTCGA GGCCATCCTG 
GACACTGTGG ATCCTAACAG GGACGGCCAC GTCTCCCTGC AAGAGTACAT GGCCTTTATG 
ATCAGCCGTG AGACCGAGAA CGTCAAGTCC AGCGAGGAGA TTGAGAGCGC CTTCCGGGCC 
CTGAGCTCTG AAGGGAAGCC TTATGTGACC AAGGAGGAGC TGTACCAGAA CCTGACTCGG 
GAACAAGCCG ACTACTGCGT CTCCCACATG AAGCCCTACG TGGACGGCAA GGGCCGCGAA 
CTCCCCACTG CTTTTGACTA CGTCGAGTTC ACCCGCTCGC TCTTCGTGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_024999033.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854801.1 Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa54551
Clone ID Related Accession (Same CDS sequence)	XM_010810459.2 , XM_024999033.1
Accession Version	XM_024999033.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7434bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT 
GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT 
CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC 
AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG 
AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC 
CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG 
AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT 
GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC 
CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC 
CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC 
CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT 
AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT 
GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG 
GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC 
ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC 
TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG 
AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG 
GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA 
GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG 
AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG 
CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG 
GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA 
ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG 
AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT 
GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT 
TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG 
CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT 
GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG 
AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG 
CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT 
CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC 
GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA 
GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG 
GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT 
CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG 
AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC 
AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC 
GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC 
CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC 
GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA 
GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC 
GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC 
AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC 
GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC 
AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGACGC GCGTAACTAA GGAGGCTGGC 
AGCGTGTCTC TGCGTATGAA ACAGGTGGAA GAACTGTACC ATTCTCTGCT GGAACTGGGA 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG 
AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC 
GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC 
AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA 
GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC 
AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG 
CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA 
GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC 
TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG 
GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC 
CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA 
TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC 
CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT 
CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG 
TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC 
GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG 
ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG 
GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG 
ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC 
GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG 
ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA 
GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG 
GACCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ATCAGAAGCA CCAGGCCTTC 
GAGGCAGAAC TGCACGCCAA TGCGGACCGG ATCCGTGGGG TCATCGACAT GGGCAACTCC 
CTCATTGACC GCGGGGCCTG CGCCGGCAGC GAGGATGCCG TCAAGGCCCG GCTGGCCGCC 
TTAGCTGACC AGTGGCAGTT CCTGGTGCAG AAGTCGGCTG AGAAGAGCCA GAAGCTGAAA 
GAAGCCAACA AGCAGCAGAA CTTCAACACT GGCATCAAGG ACTTCGACTT CTGGCTCTCT 
GAGGTGGAAG CCCTGCTGGC CTCTGAGGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC 
TTGCTAAAAA AACATCAGCT GCTGGAGGCA GATATCTCTG CCCACGAGGA CCGCCTGAAG 
GACCTCAACA GCCAGGCGGA CAGCCTGATG ACCAGCAGCG CCTTCGACAC CTCCCAGGTG 
AAGGACAAGC GGGACGCCAT CAACGGCCGT TTCCAGAAGA TCAAGAGCAT GGCCGCCTCG 
AGGCGAGCTA GGCTGAATGA GTCCCACCGC CTGCACCAGT TTTTCCGGGA CATGGATGAT 
GAGGAGTCCT GGATCAAGGA AAAGAAGCTG CTGGTGAGCT CAGAGGACTA CGGCCGTGAC 
CTGACGGGCG TCCAGAACCT GAGGAAGAAG CACAAACGGC TGGAAGCAGA GCTGGCTGCC 
CACGAGCCGG CCATTCAGGG TGTCCTGGAC ACTGGAAAGA AGCTGTCTGA TGACAACACC 
ATCGGGAAGG AGGAGATTCA GCAGCGCCTG GCGCAGTTCG TGGAGCACTG GAAGGAGCTG 
AAGCAGCTGG CAGCTGCCCG GGGCCAGCGG CTAGAAGAAT CTTTGGAATA TCAGCAGTTT 
GTAGCCAACG TGGAGGAGGA GGAAGCCTGG ATCAATGAGA AGATGACCCT GGTGGCCAGT 
GAAGATTATG GAGACACTCT TGCTGCCATC CAGGGCTTGC TGAAGAAACA CGAAGCCTTC 
GAGACGGACT TCACTGTCCA CAAGGACCGA GTGAATGATG TCTGCACCAA CGGCCAGGAC 
CTGGTTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CCAAGATGAA GGGCTTGAAC 
GGGAAAGTGT CAGACTTGGA GAAGGCTGCC TCCCAGAGGA AGGCCAAGCT CGACGAGAAC 
TCGGCCTTCC TCCAGTTCAA CTGGAAAGCC GATGTGGTGG AGTCCTGGAT CGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGT CGAGATCTGT CTTCTGTCCA AACTCTCCTC 
ACCAAACAGG AAACCTTCGA TGCTGGCCTG CAGGCCTTCC AGCAGGAGGG CATCGCCAAC 
ATCACTGCCC TCAAAGATCA GCTGCTGGCC GCCAAGCACG TTCAGTCCAA GGCCATCGAG 
GCCCGCCATG CTGCCCTCAT GAAGAGGTGG AGCCAGCTCC TGGCCAACTC GGCCACCCGG 
AAGAAGAAGC TGCTGGAGGC ACAGAGTCAC TTCCGCAAGG TGGAGGATCT CTTCCTGACC 
TTTGCTAAAA AGGCTTCAGC CTTCAACAGC TGGTTTGAAA ACGCAGAGGA GGACTTAACC 
GACCCAGTGC GCTGCAACTC TCTGGAAGAG ATCAAGGCCT TGAGAGAGGC CCACGACGCC 
TTCCGCTCCT CGTTGAGCTC GGCCCAGGCC GACTTCAACC AGCTGGCAGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT GGCCTCCAAC CCCTACACCT GGTTCACCAT GGAGGCCCTG 
GAGGAGACCT GGAGAAACCT ACAGAAGATC ATCAAGGAAA GGGAGCTGGA GCTGCAGAAG 
GAGCAGCGAC GGCAGGAGGA GAATGACAAG CTGCGCCAGG AGTTCGCCCA GCATGCCAAC 
GCCTTCCACC AGTGGATCCA GGAGACCAGA ACGTACCTCC TCGATGGGTC CTGCATGGTG 
GAAGAATCCG GGACCCTCGA ATCACAGCTT GAAGCTACCA AACGCAAACA CCAGGAGATT 
CGAGCTATGA GAAGTCAGCT CAAGAAGATT GAAGACCTGG GGGCGGCCAT GGAGGAAGCG 
CTGATCCTGG ACAACAAGTA CACGGAGCAC AGCACCGTGG GCCTGGCCCA GCAGTGGGAC 
CAGCTCGACC AGTTGGGCAT GCGCATGCAG CACAACCTGG AGCAGCAGAT CCAGGCCAGG 
AACACGACCG GAGTGACCGA GGAGGCCCTC AAAGAATTCA GCATGATGTT CAAACACTTC 
GACAAGGACA AGTCTGGCCG GCTGAACCAC CAGGAGTTCA AATCCTGTCT GCGCTCCCTG 
GGCTATGACC TGCCCATGGT GGAGGAAGGG GAGCCCGACC CTGAGTTCGA GGCCATCCTG 
GACACTGTGG ATCCTAACAG GGACGGCCAC GTCTCCCTGC AAGAGTACAT GGCCTTTATG 
ATCAGCCGTG AGACCGAGAA CGTCAAGTCC AGCGAGGAGA TTGAGAGCGC CTTCCGGGCC 
CTGAGCTCTG AAGGGAAGCC TTATGTGACC AAGGAGGAGC TGTACCAGAA CCTGACTCGG 
GAACAAGCCG ACTACTGCGT CTCCCACATG AAGCCCTACG TGGACGGCAA GGGCCGCGAA 
CTCCCCACTG CTTTTGACTA CGTCGAGTTC ACCCGCTCGC TCTTCGTGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854801.1
CDS	165..7598
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLHQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMYRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVDHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWESLKSKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRVTKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGAMPRDESDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKEKLDVLDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQSFADQLIAGGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDMGNSLIDRGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDAINGRFQKIKSMAASRRARLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLVKKNNHHEENISSKMKGLNGKVSDLEKAASQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHAALMKRWSQLLANSATRKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_024999033.1
Organism	Bos taurus(cattle)
Definition	Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999033.1

ORF Insert Sequence:

ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT 
GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT 
CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC 
AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG 
AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC 
CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG 
AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT 
GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC 
CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC 
CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC 
CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT 
AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT 
GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG 
GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC 
ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC 
TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG 
AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG 
GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA 
GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG 
AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG 
CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG 
GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA 
ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG 
AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT 
GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT 
TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG 
CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT 
GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG 
AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG 
CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT 
CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC 
GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA 
GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG 
GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT 
CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG 
AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC 
AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC 
GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC 
CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC 
GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA 
GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC 
GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC 
AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC 
GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC 
AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGACGC GCGTAACTAA GGAGGCTGGC 
AGCGTGTCTC TGCGTATGAA ACAGGTGGAA GAACTGTACC ATTCTCTGCT GGAACTGGGA 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG 
AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC 
GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC 
AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA 
GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC 
AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG 
CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA 
GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC 
TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG 
GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC 
CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA 
TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC 
CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT 
CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG 
TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC 
GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG 
ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG 
GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG 
ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC 
GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG 
ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA 
GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG 
GACCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ATCAGAAGCA CCAGGCCTTC 
GAGGCAGAAC TGCACGCCAA TGCGGACCGG ATCCGTGGGG TCATCGACAT GGGCAACTCC 
CTCATTGACC GCGGGGCCTG CGCCGGCAGC GAGGATGCCG TCAAGGCCCG GCTGGCCGCC 
TTAGCTGACC AGTGGCAGTT CCTGGTGCAG AAGTCGGCTG AGAAGAGCCA GAAGCTGAAA 
GAAGCCAACA AGCAGCAGAA CTTCAACACT GGCATCAAGG ACTTCGACTT CTGGCTCTCT 
GAGGTGGAAG CCCTGCTGGC CTCTGAGGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC 
TTGCTAAAAA AACATCAGCT GCTGGAGGCA GATATCTCTG CCCACGAGGA CCGCCTGAAG 
GACCTCAACA GCCAGGCGGA CAGCCTGATG ACCAGCAGCG CCTTCGACAC CTCCCAGGTG 
AAGGACAAGC GGGACGCCAT CAACGGCCGT TTCCAGAAGA TCAAGAGCAT GGCCGCCTCG 
AGGCGAGCTA GGCTGAATGA GTCCCACCGC CTGCACCAGT TTTTCCGGGA CATGGATGAT 
GAGGAGTCCT GGATCAAGGA AAAGAAGCTG CTGGTGAGCT CAGAGGACTA CGGCCGTGAC 
CTGACGGGCG TCCAGAACCT GAGGAAGAAG CACAAACGGC TGGAAGCAGA GCTGGCTGCC 
CACGAGCCGG CCATTCAGGG TGTCCTGGAC ACTGGAAAGA AGCTGTCTGA TGACAACACC 
ATCGGGAAGG AGGAGATTCA GCAGCGCCTG GCGCAGTTCG TGGAGCACTG GAAGGAGCTG 
AAGCAGCTGG CAGCTGCCCG GGGCCAGCGG CTAGAAGAAT CTTTGGAATA TCAGCAGTTT 
GTAGCCAACG TGGAGGAGGA GGAAGCCTGG ATCAATGAGA AGATGACCCT GGTGGCCAGT 
GAAGATTATG GAGACACTCT TGCTGCCATC CAGGGCTTGC TGAAGAAACA CGAAGCCTTC 
GAGACGGACT TCACTGTCCA CAAGGACCGA GTGAATGATG TCTGCACCAA CGGCCAGGAC 
CTGGTTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CCAAGATGAA GGGCTTGAAC 
GGGAAAGTGT CAGACTTGGA GAAGGCTGCC TCCCAGAGGA AGGCCAAGCT CGACGAGAAC 
TCGGCCTTCC TCCAGTTCAA CTGGAAAGCC GATGTGGTGG AGTCCTGGAT CGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGT CGAGATCTGT CTTCTGTCCA AACTCTCCTC 
ACCAAACAGG AAACCTTCGA TGCTGGCCTG CAGGCCTTCC AGCAGGAGGG CATCGCCAAC 
ATCACTGCCC TCAAAGATCA GCTGCTGGCC GCCAAGCACG TTCAGTCCAA GGCCATCGAG 
GCCCGCCATG CTGCCCTCAT GAAGAGGTGG AGCCAGCTCC TGGCCAACTC GGCCACCCGG 
AAGAAGAAGC TGCTGGAGGC ACAGAGTCAC TTCCGCAAGG TGGAGGATCT CTTCCTGACC 
TTTGCTAAAA AGGCTTCAGC CTTCAACAGC TGGTTTGAAA ACGCAGAGGA GGACTTAACC 
GACCCAGTGC GCTGCAACTC TCTGGAAGAG ATCAAGGCCT TGAGAGAGGC CCACGACGCC 
TTCCGCTCCT CGTTGAGCTC GGCCCAGGCC GACTTCAACC AGCTGGCAGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT GGCCTCCAAC CCCTACACCT GGTTCACCAT GGAGGCCCTG 
GAGGAGACCT GGAGAAACCT ACAGAAGATC ATCAAGGAAA GGGAGCTGGA GCTGCAGAAG 
GAGCAGCGAC GGCAGGAGGA GAATGACAAG CTGCGCCAGG AGTTCGCCCA GCATGCCAAC 
GCCTTCCACC AGTGGATCCA GGAGACCAGA ACGTACCTCC TCGATGGGTC CTGCATGGTG 
GAAGAATCCG GGACCCTCGA ATCACAGCTT GAAGCTACCA AACGCAAACA CCAGGAGATT 
CGAGCTATGA GAAGTCAGCT CAAGAAGATT GAAGACCTGG GGGCGGCCAT GGAGGAAGCG 
CTGATCCTGG ACAACAAGTA CACGGAGCAC AGCACCGTGG GCCTGGCCCA GCAGTGGGAC 
CAGCTCGACC AGTTGGGCAT GCGCATGCAG CACAACCTGG AGCAGCAGAT CCAGGCCAGG 
AACACGACCG GAGTGACCGA GGAGGCCCTC AAAGAATTCA GCATGATGTT CAAACACTTC 
GACAAGGACA AGTCTGGCCG GCTGAACCAC CAGGAGTTCA AATCCTGTCT GCGCTCCCTG 
GGCTATGACC TGCCCATGGT GGAGGAAGGG GAGCCCGACC CTGAGTTCGA GGCCATCCTG 
GACACTGTGG ATCCTAACAG GGACGGCCAC GTCTCCCTGC AAGAGTACAT GGCCTTTATG 
ATCAGCCGTG AGACCGAGAA CGTCAAGTCC AGCGAGGAGA TTGAGAGCGC CTTCCGGGCC 
CTGAGCTCTG AAGGGAAGCC TTATGTGACC AAGGAGGAGC TGTACCAGAA CCTGACTCGG 
GAACAAGCCG ACTACTGCGT CTCCCACATG AAGCCCTACG TGGACGGCAA GGGCCGCGAA 
CTCCCCACTG CTTTTGACTA CGTCGAGTTC ACCCGCTCGC TCTTCGTGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_010810461.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010808763.1 Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OBa54553
Clone ID Related Accession (Same CDS sequence)	XM_010810461.2 , XM_024999040.1
Accession Version	XM_010810461.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810461.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT 
GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT 
CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC 
AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG 
AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC 
CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG 
AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT 
GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC 
CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC 
CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC 
CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT 
AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT 
GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG 
GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC 
ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC 
TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG 
AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG 
GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA 
GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG 
AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG 
CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG 
GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA 
ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG 
AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT 
GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT 
TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG 
CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT 
GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG 
AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG 
CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT 
CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC 
GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA 
GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG 
GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT 
CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG 
AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC 
AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC 
GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC 
CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC 
GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA 
GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC 
GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC 
AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC 
GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC 
AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGTACC ATTCTCTGCT GGAACTGGGA 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG 
AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC 
GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC 
AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA 
GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC 
AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG 
CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA 
GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC 
TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG 
GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC 
CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA 
TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC 
CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT 
CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG 
TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC 
GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG 
ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG 
GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG 
ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC 
GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG 
ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA 
GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG 
GACCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ATCAGAAGCA CCAGGCCTTC 
GAGGCAGAAC TGCACGCCAA TGCGGACCGG ATCCGTGGGG TCATCGACAT GGGCAACTCC 
CTCATTGACC GCGGGGCCTG CGCCGGCAGC GAGGATGCCG TCAAGGCCCG GCTGGCCGCC 
TTAGCTGACC AGTGGCAGTT CCTGGTGCAG AAGTCGGCTG AGAAGAGCCA GAAGCTGAAA 
GAAGCCAACA AGCAGCAGAA CTTCAACACT GGCATCAAGG ACTTCGACTT CTGGCTCTCT 
GAGGTGGAAG CCCTGCTGGC CTCTGAGGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC 
TTGCTAAAAA AACATCAGCT GCTGGAGGCA GATATCTCTG CCCACGAGGA CCGCCTGAAG 
GACCTCAACA GCCAGGCGGA CAGCCTGATG ACCAGCAGCG CCTTCGACAC CTCCCAGGTG 
AAGGACAAGC GGGACGCCAT CAACGGCCGT TTCCAGAAGA TCAAGAGCAT GGCCGCCTCG 
AGGCGAGCTA GGCTGAATGA GTCCCACCGC CTGCACCAGT TTTTCCGGGA CATGGATGAT 
GAGGAGTCCT GGATCAAGGA AAAGAAGCTG CTGGTGAGCT CAGAGGACTA CGGCCGTGAC 
CTGACGGGCG TCCAGAACCT GAGGAAGAAG CACAAACGGC TGGAAGCAGA GCTGGCTGCC 
CACGAGCCGG CCATTCAGGG TGTCCTGGAC ACTGGAAAGA AGCTGTCTGA TGACAACACC 
ATCGGGAAGG AGGAGATTCA GCAGCGCCTG GCGCAGTTCG TGGAGCACTG GAAGGAGCTG 
AAGCAGCTGG CAGCTGCCCG GGGCCAGCGG CTAGAAGAAT CTTTGGAATA TCAGCAGTTT 
GTAGCCAACG TGGAGGAGGA GGAAGCCTGG ATCAATGAGA AGATGACCCT GGTGGCCAGT 
GAAGATTATG GAGACACTCT TGCTGCCATC CAGGGCTTGC TGAAGAAACA CGAAGCCTTC 
GAGACGGACT TCACTGTCCA CAAGGACCGA GTGAATGATG TCTGCACCAA CGGCCAGGAC 
CTGGTTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CCAAGATGAA GGGCTTGAAC 
GGGAAAGTGT CAGACTTGGA GAAGGCTGCC TCCCAGAGGA AGGCCAAGCT CGACGAGAAC 
TCGGCCTTCC TCCAGTTCAA CTGGAAAGCC GATGTGGTGG AGTCCTGGAT CGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGT CGAGATCTGT CTTCTGTCCA AACTCTCCTC 
ACCAAACAGG AAACCTTCGA TGCTGGCCTG CAGGCCTTCC AGCAGGAGGG CATCGCCAAC 
ATCACTGCCC TCAAAGATCA GCTGCTGGCC GCCAAGCACG TTCAGTCCAA GGCCATCGAG 
GCCCGCCATG CTGCCCTCAT GAAGAGGTGG AGCCAGCTCC TGGCCAACTC GGCCACCCGG 
AAGAAGAAGC TGCTGGAGGC ACAGAGTCAC TTCCGCAAGG TGGAGGATCT CTTCCTGACC 
TTTGCTAAAA AGGCTTCAGC CTTCAACAGC TGGTTTGAAA ACGCAGAGGA GGACTTAACC 
GACCCAGTGC GCTGCAACTC TCTGGAAGAG ATCAAGGCCT TGAGAGAGGC CCACGACGCC 
TTCCGCTCCT CGTTGAGCTC GGCCCAGGCC GACTTCAACC AGCTGGCAGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT GGCCTCCAAC CCCTACACCT GGTTCACCAT GGAGGCCCTG 
GAGGAGACCT GGAGAAACCT ACAGAAGATC ATCAAGGAAA GGGAGCTGGA GCTGCAGAAG 
GAGCAGCGAC GGCAGGAGGA GAATGACAAG CTGCGCCAGG AGTTCGCCCA GCATGCCAAC 
GCCTTCCACC AGTGGATCCA GGAGACCAGA ACGTACCTCC TCGATGGGTC CTGCATGGTG 
GAAGAATCCG GGACCCTCGA ATCACAGCTT GAAGCTACCA AACGCAAACA CCAGGAGATT 
CGAGCTATGA GAAGTCAGCT CAAGAAGATT GAAGACCTGG GGGCGGCCAT GGAGGAAGCG 
CTGATCCTGG ACAACAAGTA CACGGAGCAC AGCACCGTGG GCCTGGCCCA GCAGTGGGAC 
CAGCTCGACC AGTTGGGCAT GCGCATGCAG CACAACCTGG AGCAGCAGAT CCAGGCCAGG 
AACACGACCG GAGTGACCGA GGAGGCCCTC AAAGAATTCA GCATGATGTT CAAACACTTC 
GACAAGGACA AGTCTGGCCG GCTGAACCAC CAGGAGTTCA AATCCTGTCT GCGCTCCCTG 
GGCTATGACC TGCCCATGGT GGAGGAAGGG GAGCCCGACC CTGAGTTCGA GGCCATCCTG 
GACACTGTGG ATCCTAACAG GGACGGCCAC GTCTCCCTGC AAGAGTACAT GGCCTTTATG 
ATCAGCCGTG AGACCGAGAA CGTCAAGTCC AGCGAGGAGA TTGAGAGCGC CTTCCGGGCC 
CTGAGCTCTG AAGGGAAGCC TTATGTGACC AAGGAGGAGC TGTACCAGAA CCTGACTCGG 
GAACAAGCCG ACTACTGCGT CTCCCACATG AAGCCCTACG TGGACGGCAA GGGCCGCGAA 
CTCCCCACTG CTTTTGACTA CGTCGAGTTC ACCCGCTCGC TCTTCGTGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010808763.1
CDS	26..7399
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLHQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMYRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVDHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWESLKSKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGAMPRDESDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKEKLDVLDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQSFADQLIAGGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDMGNSLIDRGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDAINGRFQKIKSMAASRRARLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLVKKNNHHEENISSKMKGLNGKVSDLEKAASQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHAALMKRWSQLLANSATRKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_010810461.2
Organism	Bos taurus(cattle)
Definition	Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010810461.2

ORF Insert Sequence:

ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT 
GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT 
CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC 
AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG 
AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC 
CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG 
AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT 
GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC 
CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC 
CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC 
CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT 
AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT 
GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG 
GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC 
ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC 
TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG 
AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG 
GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA 
GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG 
AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG 
CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG 
GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA 
ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG 
AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT 
GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT 
TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG 
CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT 
GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG 
AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG 
CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT 
CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC 
GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA 
GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG 
GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT 
CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG 
AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC 
AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC 
GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC 
CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC 
GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA 
GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC 
GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC 
AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC 
GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC 
AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGTACC ATTCTCTGCT GGAACTGGGA 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG 
AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC 
GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC 
AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA 
GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC 
AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG 
CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA 
GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC 
TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG 
GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC 
CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA 
TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC 
CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT 
CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG 
TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC 
GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG 
ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG 
GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG 
ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC 
GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG 
ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA 
GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG 
GACCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ATCAGAAGCA CCAGGCCTTC 
GAGGCAGAAC TGCACGCCAA TGCGGACCGG ATCCGTGGGG TCATCGACAT GGGCAACTCC 
CTCATTGACC GCGGGGCCTG CGCCGGCAGC GAGGATGCCG TCAAGGCCCG GCTGGCCGCC 
TTAGCTGACC AGTGGCAGTT CCTGGTGCAG AAGTCGGCTG AGAAGAGCCA GAAGCTGAAA 
GAAGCCAACA AGCAGCAGAA CTTCAACACT GGCATCAAGG ACTTCGACTT CTGGCTCTCT 
GAGGTGGAAG CCCTGCTGGC CTCTGAGGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC 
TTGCTAAAAA AACATCAGCT GCTGGAGGCA GATATCTCTG CCCACGAGGA CCGCCTGAAG 
GACCTCAACA GCCAGGCGGA CAGCCTGATG ACCAGCAGCG CCTTCGACAC CTCCCAGGTG 
AAGGACAAGC GGGACGCCAT CAACGGCCGT TTCCAGAAGA TCAAGAGCAT GGCCGCCTCG 
AGGCGAGCTA GGCTGAATGA GTCCCACCGC CTGCACCAGT TTTTCCGGGA CATGGATGAT 
GAGGAGTCCT GGATCAAGGA AAAGAAGCTG CTGGTGAGCT CAGAGGACTA CGGCCGTGAC 
CTGACGGGCG TCCAGAACCT GAGGAAGAAG CACAAACGGC TGGAAGCAGA GCTGGCTGCC 
CACGAGCCGG CCATTCAGGG TGTCCTGGAC ACTGGAAAGA AGCTGTCTGA TGACAACACC 
ATCGGGAAGG AGGAGATTCA GCAGCGCCTG GCGCAGTTCG TGGAGCACTG GAAGGAGCTG 
AAGCAGCTGG CAGCTGCCCG GGGCCAGCGG CTAGAAGAAT CTTTGGAATA TCAGCAGTTT 
GTAGCCAACG TGGAGGAGGA GGAAGCCTGG ATCAATGAGA AGATGACCCT GGTGGCCAGT 
GAAGATTATG GAGACACTCT TGCTGCCATC CAGGGCTTGC TGAAGAAACA CGAAGCCTTC 
GAGACGGACT TCACTGTCCA CAAGGACCGA GTGAATGATG TCTGCACCAA CGGCCAGGAC 
CTGGTTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CCAAGATGAA GGGCTTGAAC 
GGGAAAGTGT CAGACTTGGA GAAGGCTGCC TCCCAGAGGA AGGCCAAGCT CGACGAGAAC 
TCGGCCTTCC TCCAGTTCAA CTGGAAAGCC GATGTGGTGG AGTCCTGGAT CGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGT CGAGATCTGT CTTCTGTCCA AACTCTCCTC 
ACCAAACAGG AAACCTTCGA TGCTGGCCTG CAGGCCTTCC AGCAGGAGGG CATCGCCAAC 
ATCACTGCCC TCAAAGATCA GCTGCTGGCC GCCAAGCACG TTCAGTCCAA GGCCATCGAG 
GCCCGCCATG CTGCCCTCAT GAAGAGGTGG AGCCAGCTCC TGGCCAACTC GGCCACCCGG 
AAGAAGAAGC TGCTGGAGGC ACAGAGTCAC TTCCGCAAGG TGGAGGATCT CTTCCTGACC 
TTTGCTAAAA AGGCTTCAGC CTTCAACAGC TGGTTTGAAA ACGCAGAGGA GGACTTAACC 
GACCCAGTGC GCTGCAACTC TCTGGAAGAG ATCAAGGCCT TGAGAGAGGC CCACGACGCC 
TTCCGCTCCT CGTTGAGCTC GGCCCAGGCC GACTTCAACC AGCTGGCAGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT GGCCTCCAAC CCCTACACCT GGTTCACCAT GGAGGCCCTG 
GAGGAGACCT GGAGAAACCT ACAGAAGATC ATCAAGGAAA GGGAGCTGGA GCTGCAGAAG 
GAGCAGCGAC GGCAGGAGGA GAATGACAAG CTGCGCCAGG AGTTCGCCCA GCATGCCAAC 
GCCTTCCACC AGTGGATCCA GGAGACCAGA ACGTACCTCC TCGATGGGTC CTGCATGGTG 
GAAGAATCCG GGACCCTCGA ATCACAGCTT GAAGCTACCA AACGCAAACA CCAGGAGATT 
CGAGCTATGA GAAGTCAGCT CAAGAAGATT GAAGACCTGG GGGCGGCCAT GGAGGAAGCG 
CTGATCCTGG ACAACAAGTA CACGGAGCAC AGCACCGTGG GCCTGGCCCA GCAGTGGGAC 
CAGCTCGACC AGTTGGGCAT GCGCATGCAG CACAACCTGG AGCAGCAGAT CCAGGCCAGG 
AACACGACCG GAGTGACCGA GGAGGCCCTC AAAGAATTCA GCATGATGTT CAAACACTTC 
GACAAGGACA AGTCTGGCCG GCTGAACCAC CAGGAGTTCA AATCCTGTCT GCGCTCCCTG 
GGCTATGACC TGCCCATGGT GGAGGAAGGG GAGCCCGACC CTGAGTTCGA GGCCATCCTG 
GACACTGTGG ATCCTAACAG GGACGGCCAC GTCTCCCTGC AAGAGTACAT GGCCTTTATG 
ATCAGCCGTG AGACCGAGAA CGTCAAGTCC AGCGAGGAGA TTGAGAGCGC CTTCCGGGCC 
CTGAGCTCTG AAGGGAAGCC TTATGTGACC AAGGAGGAGC TGTACCAGAA CCTGACTCGG 
GAACAAGCCG ACTACTGCGT CTCCCACATG AAGCCCTACG TGGACGGCAA GGGCCGCGAA 
CTCCCCACTG CTTTTGACTA CGTCGAGTTC ACCCGCTCGC TCTTCGTGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_024999040.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854808.1 Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OBa54553
Clone ID Related Accession (Same CDS sequence)	XM_010810461.2 , XM_024999040.1
Accession Version	XM_024999040.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT 
GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT 
CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC 
AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG 
AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC 
CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG 
AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT 
GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC 
CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC 
CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC 
CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT 
AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT 
GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG 
GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC 
ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC 
TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG 
AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG 
GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA 
GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG 
AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG 
CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG 
GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA 
ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG 
AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT 
GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT 
TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG 
CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT 
GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG 
AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG 
CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT 
CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC 
GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA 
GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG 
GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT 
CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG 
AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC 
AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC 
GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC 
CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC 
GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA 
GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC 
GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC 
AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC 
GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC 
AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGTACC ATTCTCTGCT GGAACTGGGA 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG 
AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC 
GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC 
AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA 
GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC 
AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG 
CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA 
GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC 
TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG 
GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC 
CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA 
TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC 
CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT 
CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG 
TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC 
GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG 
ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG 
GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG 
ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC 
GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG 
ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA 
GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG 
GACCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ATCAGAAGCA CCAGGCCTTC 
GAGGCAGAAC TGCACGCCAA TGCGGACCGG ATCCGTGGGG TCATCGACAT GGGCAACTCC 
CTCATTGACC GCGGGGCCTG CGCCGGCAGC GAGGATGCCG TCAAGGCCCG GCTGGCCGCC 
TTAGCTGACC AGTGGCAGTT CCTGGTGCAG AAGTCGGCTG AGAAGAGCCA GAAGCTGAAA 
GAAGCCAACA AGCAGCAGAA CTTCAACACT GGCATCAAGG ACTTCGACTT CTGGCTCTCT 
GAGGTGGAAG CCCTGCTGGC CTCTGAGGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC 
TTGCTAAAAA AACATCAGCT GCTGGAGGCA GATATCTCTG CCCACGAGGA CCGCCTGAAG 
GACCTCAACA GCCAGGCGGA CAGCCTGATG ACCAGCAGCG CCTTCGACAC CTCCCAGGTG 
AAGGACAAGC GGGACGCCAT CAACGGCCGT TTCCAGAAGA TCAAGAGCAT GGCCGCCTCG 
AGGCGAGCTA GGCTGAATGA GTCCCACCGC CTGCACCAGT TTTTCCGGGA CATGGATGAT 
GAGGAGTCCT GGATCAAGGA AAAGAAGCTG CTGGTGAGCT CAGAGGACTA CGGCCGTGAC 
CTGACGGGCG TCCAGAACCT GAGGAAGAAG CACAAACGGC TGGAAGCAGA GCTGGCTGCC 
CACGAGCCGG CCATTCAGGG TGTCCTGGAC ACTGGAAAGA AGCTGTCTGA TGACAACACC 
ATCGGGAAGG AGGAGATTCA GCAGCGCCTG GCGCAGTTCG TGGAGCACTG GAAGGAGCTG 
AAGCAGCTGG CAGCTGCCCG GGGCCAGCGG CTAGAAGAAT CTTTGGAATA TCAGCAGTTT 
GTAGCCAACG TGGAGGAGGA GGAAGCCTGG ATCAATGAGA AGATGACCCT GGTGGCCAGT 
GAAGATTATG GAGACACTCT TGCTGCCATC CAGGGCTTGC TGAAGAAACA CGAAGCCTTC 
GAGACGGACT TCACTGTCCA CAAGGACCGA GTGAATGATG TCTGCACCAA CGGCCAGGAC 
CTGGTTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CCAAGATGAA GGGCTTGAAC 
GGGAAAGTGT CAGACTTGGA GAAGGCTGCC TCCCAGAGGA AGGCCAAGCT CGACGAGAAC 
TCGGCCTTCC TCCAGTTCAA CTGGAAAGCC GATGTGGTGG AGTCCTGGAT CGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGT CGAGATCTGT CTTCTGTCCA AACTCTCCTC 
ACCAAACAGG AAACCTTCGA TGCTGGCCTG CAGGCCTTCC AGCAGGAGGG CATCGCCAAC 
ATCACTGCCC TCAAAGATCA GCTGCTGGCC GCCAAGCACG TTCAGTCCAA GGCCATCGAG 
GCCCGCCATG CTGCCCTCAT GAAGAGGTGG AGCCAGCTCC TGGCCAACTC GGCCACCCGG 
AAGAAGAAGC TGCTGGAGGC ACAGAGTCAC TTCCGCAAGG TGGAGGATCT CTTCCTGACC 
TTTGCTAAAA AGGCTTCAGC CTTCAACAGC TGGTTTGAAA ACGCAGAGGA GGACTTAACC 
GACCCAGTGC GCTGCAACTC TCTGGAAGAG ATCAAGGCCT TGAGAGAGGC CCACGACGCC 
TTCCGCTCCT CGTTGAGCTC GGCCCAGGCC GACTTCAACC AGCTGGCAGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT GGCCTCCAAC CCCTACACCT GGTTCACCAT GGAGGCCCTG 
GAGGAGACCT GGAGAAACCT ACAGAAGATC ATCAAGGAAA GGGAGCTGGA GCTGCAGAAG 
GAGCAGCGAC GGCAGGAGGA GAATGACAAG CTGCGCCAGG AGTTCGCCCA GCATGCCAAC 
GCCTTCCACC AGTGGATCCA GGAGACCAGA ACGTACCTCC TCGATGGGTC CTGCATGGTG 
GAAGAATCCG GGACCCTCGA ATCACAGCTT GAAGCTACCA AACGCAAACA CCAGGAGATT 
CGAGCTATGA GAAGTCAGCT CAAGAAGATT GAAGACCTGG GGGCGGCCAT GGAGGAAGCG 
CTGATCCTGG ACAACAAGTA CACGGAGCAC AGCACCGTGG GCCTGGCCCA GCAGTGGGAC 
CAGCTCGACC AGTTGGGCAT GCGCATGCAG CACAACCTGG AGCAGCAGAT CCAGGCCAGG 
AACACGACCG GAGTGACCGA GGAGGCCCTC AAAGAATTCA GCATGATGTT CAAACACTTC 
GACAAGGACA AGTCTGGCCG GCTGAACCAC CAGGAGTTCA AATCCTGTCT GCGCTCCCTG 
GGCTATGACC TGCCCATGGT GGAGGAAGGG GAGCCCGACC CTGAGTTCGA GGCCATCCTG 
GACACTGTGG ATCCTAACAG GGACGGCCAC GTCTCCCTGC AAGAGTACAT GGCCTTTATG 
ATCAGCCGTG AGACCGAGAA CGTCAAGTCC AGCGAGGAGA TTGAGAGCGC CTTCCGGGCC 
CTGAGCTCTG AAGGGAAGCC TTATGTGACC AAGGAGGAGC TGTACCAGAA CCTGACTCGG 
GAACAAGCCG ACTACTGCGT CTCCCACATG AAGCCCTACG TGGACGGCAA GGGCCGCGAA 
CTCCCCACTG CTTTTGACTA CGTCGAGTTC ACCCGCTCGC TCTTCGTGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854808.1
CDS	126..7499
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLHQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMYRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVDHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWESLKSKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGAMPRDESDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKEKLDVLDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQSFADQLIAGGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDMGNSLIDRGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDAINGRFQKIKSMAASRRARLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLVKKNNHHEENISSKMKGLNGKVSDLEKAASQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHAALMKRWSQLLANSATRKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_024999040.1
Organism	Bos taurus(cattle)
Definition	Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999040.1

ORF Insert Sequence:

ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT 
GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT 
CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC 
AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG 
AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC 
CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG 
AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT 
GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC 
CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC 
CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC 
CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT 
AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT 
GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG 
GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC 
ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC 
TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG 
AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG 
GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA 
GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG 
AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG 
CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG 
GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA 
ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG 
AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT 
GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT 
TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG 
CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT 
GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG 
AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG 
CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT 
CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC 
GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA 
GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG 
GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT 
CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG 
AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC 
AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC 
GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC 
CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC 
GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA 
GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC 
GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC 
AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC 
GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC 
AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGTACC ATTCTCTGCT GGAACTGGGA 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG 
AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC 
GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC 
AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA 
GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC 
AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG 
CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA 
GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC 
TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG 
GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC 
CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA 
TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC 
CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT 
CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG 
TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC 
GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG 
ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG 
GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG 
ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC 
GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG 
ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA 
GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG 
GACCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ATCAGAAGCA CCAGGCCTTC 
GAGGCAGAAC TGCACGCCAA TGCGGACCGG ATCCGTGGGG TCATCGACAT GGGCAACTCC 
CTCATTGACC GCGGGGCCTG CGCCGGCAGC GAGGATGCCG TCAAGGCCCG GCTGGCCGCC 
TTAGCTGACC AGTGGCAGTT CCTGGTGCAG AAGTCGGCTG AGAAGAGCCA GAAGCTGAAA 
GAAGCCAACA AGCAGCAGAA CTTCAACACT GGCATCAAGG ACTTCGACTT CTGGCTCTCT 
GAGGTGGAAG CCCTGCTGGC CTCTGAGGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC 
TTGCTAAAAA AACATCAGCT GCTGGAGGCA GATATCTCTG CCCACGAGGA CCGCCTGAAG 
GACCTCAACA GCCAGGCGGA CAGCCTGATG ACCAGCAGCG CCTTCGACAC CTCCCAGGTG 
AAGGACAAGC GGGACGCCAT CAACGGCCGT TTCCAGAAGA TCAAGAGCAT GGCCGCCTCG 
AGGCGAGCTA GGCTGAATGA GTCCCACCGC CTGCACCAGT TTTTCCGGGA CATGGATGAT 
GAGGAGTCCT GGATCAAGGA AAAGAAGCTG CTGGTGAGCT CAGAGGACTA CGGCCGTGAC 
CTGACGGGCG TCCAGAACCT GAGGAAGAAG CACAAACGGC TGGAAGCAGA GCTGGCTGCC 
CACGAGCCGG CCATTCAGGG TGTCCTGGAC ACTGGAAAGA AGCTGTCTGA TGACAACACC 
ATCGGGAAGG AGGAGATTCA GCAGCGCCTG GCGCAGTTCG TGGAGCACTG GAAGGAGCTG 
AAGCAGCTGG CAGCTGCCCG GGGCCAGCGG CTAGAAGAAT CTTTGGAATA TCAGCAGTTT 
GTAGCCAACG TGGAGGAGGA GGAAGCCTGG ATCAATGAGA AGATGACCCT GGTGGCCAGT 
GAAGATTATG GAGACACTCT TGCTGCCATC CAGGGCTTGC TGAAGAAACA CGAAGCCTTC 
GAGACGGACT TCACTGTCCA CAAGGACCGA GTGAATGATG TCTGCACCAA CGGCCAGGAC 
CTGGTTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CCAAGATGAA GGGCTTGAAC 
GGGAAAGTGT CAGACTTGGA GAAGGCTGCC TCCCAGAGGA AGGCCAAGCT CGACGAGAAC 
TCGGCCTTCC TCCAGTTCAA CTGGAAAGCC GATGTGGTGG AGTCCTGGAT CGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGT CGAGATCTGT CTTCTGTCCA AACTCTCCTC 
ACCAAACAGG AAACCTTCGA TGCTGGCCTG CAGGCCTTCC AGCAGGAGGG CATCGCCAAC 
ATCACTGCCC TCAAAGATCA GCTGCTGGCC GCCAAGCACG TTCAGTCCAA GGCCATCGAG 
GCCCGCCATG CTGCCCTCAT GAAGAGGTGG AGCCAGCTCC TGGCCAACTC GGCCACCCGG 
AAGAAGAAGC TGCTGGAGGC ACAGAGTCAC TTCCGCAAGG TGGAGGATCT CTTCCTGACC 
TTTGCTAAAA AGGCTTCAGC CTTCAACAGC TGGTTTGAAA ACGCAGAGGA GGACTTAACC 
GACCCAGTGC GCTGCAACTC TCTGGAAGAG ATCAAGGCCT TGAGAGAGGC CCACGACGCC 
TTCCGCTCCT CGTTGAGCTC GGCCCAGGCC GACTTCAACC AGCTGGCAGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT GGCCTCCAAC CCCTACACCT GGTTCACCAT GGAGGCCCTG 
GAGGAGACCT GGAGAAACCT ACAGAAGATC ATCAAGGAAA GGGAGCTGGA GCTGCAGAAG 
GAGCAGCGAC GGCAGGAGGA GAATGACAAG CTGCGCCAGG AGTTCGCCCA GCATGCCAAC 
GCCTTCCACC AGTGGATCCA GGAGACCAGA ACGTACCTCC TCGATGGGTC CTGCATGGTG 
GAAGAATCCG GGACCCTCGA ATCACAGCTT GAAGCTACCA AACGCAAACA CCAGGAGATT 
CGAGCTATGA GAAGTCAGCT CAAGAAGATT GAAGACCTGG GGGCGGCCAT GGAGGAAGCG 
CTGATCCTGG ACAACAAGTA CACGGAGCAC AGCACCGTGG GCCTGGCCCA GCAGTGGGAC 
CAGCTCGACC AGTTGGGCAT GCGCATGCAG CACAACCTGG AGCAGCAGAT CCAGGCCAGG 
AACACGACCG GAGTGACCGA GGAGGCCCTC AAAGAATTCA GCATGATGTT CAAACACTTC 
GACAAGGACA AGTCTGGCCG GCTGAACCAC CAGGAGTTCA AATCCTGTCT GCGCTCCCTG 
GGCTATGACC TGCCCATGGT GGAGGAAGGG GAGCCCGACC CTGAGTTCGA GGCCATCCTG 
GACACTGTGG ATCCTAACAG GGACGGCCAC GTCTCCCTGC AAGAGTACAT GGCCTTTATG 
ATCAGCCGTG AGACCGAGAA CGTCAAGTCC AGCGAGGAGA TTGAGAGCGC CTTCCGGGCC 
CTGAGCTCTG AAGGGAAGCC TTATGTGACC AAGGAGGAGC TGTACCAGAA CCTGACTCGG 
GAACAAGCCG ACTACTGCGT CTCCCACATG AAGCCCTACG TGGACGGCAA GGGCCGCGAA 
CTCCCCACTG CTTTTGACTA CGTCGAGTTC ACCCGCTCGC TCTTCGTGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_010810462.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010808764.1 Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OBa54554
Clone ID Related Accession (Same CDS sequence)	XM_010810462.2 , XM_024999042.1
Accession Version	XM_010810462.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810462.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT 
GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT 
CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC 
AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG 
AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC 
CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG 
AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT 
GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC 
CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC 
CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC 
CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT 
AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT 
GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG 
GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC 
ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC 
TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG 
AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG 
GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA 
GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG 
AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG 
CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG 
GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA 
ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG 
AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT 
GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT 
TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG 
CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT 
GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG 
AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG 
CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT 
CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC 
GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA 
GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG 
GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT 
CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG 
AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC 
AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC 
GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC 
CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC 
GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA 
GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC 
GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC 
AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC 
GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC 
AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGTACC ATTCTCTGCT GGAACTGGGA 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG 
AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC 
GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC 
AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA 
GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC 
AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG 
CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA 
GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC 
TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG 
GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC 
CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA 
TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC 
CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT 
CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG 
TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC 
GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG 
ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG 
GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG 
ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC 
GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG 
ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA 
GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG 
GACCCCACCA ACATCCAGAG CAAGCATCAG AAGCACCAGG CCTTCGAGGC AGAACTGCAC 
GCCAATGCGG ACCGGATCCG TGGGGTCATC GACATGGGCA ACTCCCTCAT TGACCGCGGG 
GCCTGCGCCG GCAGCGAGGA TGCCGTCAAG GCCCGGCTGG CCGCCTTAGC TGACCAGTGG 
CAGTTCCTGG TGCAGAAGTC GGCTGAGAAG AGCCAGAAGC TGAAAGAAGC CAACAAGCAG 
CAGAACTTCA ACACTGGCAT CAAGGACTTC GACTTCTGGC TCTCTGAGGT GGAAGCCCTG 
CTGGCCTCTG AGGATTATGG CAAAGACCTG GCTTCTGTGA ACAACTTGCT AAAAAAACAT 
CAGCTGCTGG AGGCAGATAT CTCTGCCCAC GAGGACCGCC TGAAGGACCT CAACAGCCAG 
GCGGACAGCC TGATGACCAG CAGCGCCTTC GACACCTCCC AGGTGAAGGA CAAGCGGGAC 
GCCATCAACG GCCGTTTCCA GAAGATCAAG AGCATGGCCG CCTCGAGGCG AGCTAGGCTG 
AATGAGTCCC ACCGCCTGCA CCAGTTTTTC CGGGACATGG ATGATGAGGA GTCCTGGATC 
AAGGAAAAGA AGCTGCTGGT GAGCTCAGAG GACTACGGCC GTGACCTGAC GGGCGTCCAG 
AACCTGAGGA AGAAGCACAA ACGGCTGGAA GCAGAGCTGG CTGCCCACGA GCCGGCCATT 
CAGGGTGTCC TGGACACTGG AAAGAAGCTG TCTGATGACA ACACCATCGG GAAGGAGGAG 
ATTCAGCAGC GCCTGGCGCA GTTCGTGGAG CACTGGAAGG AGCTGAAGCA GCTGGCAGCT 
GCCCGGGGCC AGCGGCTAGA AGAATCTTTG GAATATCAGC AGTTTGTAGC CAACGTGGAG 
GAGGAGGAAG CCTGGATCAA TGAGAAGATG ACCCTGGTGG CCAGTGAAGA TTATGGAGAC 
ACTCTTGCTG CCATCCAGGG CTTGCTGAAG AAACACGAAG CCTTCGAGAC GGACTTCACT 
GTCCACAAGG ACCGAGTGAA TGATGTCTGC ACCAACGGCC AGGACCTGGT TAAGAAGAAC 
AATCACCATG AGGAGAACAT CTCTTCCAAG ATGAAGGGCT TGAACGGGAA AGTGTCAGAC 
TTGGAGAAGG CTGCCTCCCA GAGGAAGGCC AAGCTCGACG AGAACTCGGC CTTCCTCCAG 
TTCAACTGGA AAGCCGATGT GGTGGAGTCC TGGATCGGTG AAAAGGAGAA CAGCTTGAAG 
ACAGATGATT ATGGTCGAGA TCTGTCTTCT GTCCAAACTC TCCTCACCAA ACAGGAAACC 
TTCGATGCTG GCCTGCAGGC CTTCCAGCAG GAGGGCATCG CCAACATCAC TGCCCTCAAA 
GATCAGCTGC TGGCCGCCAA GCACGTTCAG TCCAAGGCCA TCGAGGCCCG CCATGCTGCC 
CTCATGAAGA GGTGGAGCCA GCTCCTGGCC AACTCGGCCA CCCGGAAGAA GAAGCTGCTG 
GAGGCACAGA GTCACTTCCG CAAGGTGGAG GATCTCTTCC TGACCTTTGC TAAAAAGGCT 
TCAGCCTTCA ACAGCTGGTT TGAAAACGCA GAGGAGGACT TAACCGACCC AGTGCGCTGC 
AACTCTCTGG AAGAGATCAA GGCCTTGAGA GAGGCCCACG ACGCCTTCCG CTCCTCGTTG 
AGCTCGGCCC AGGCCGACTT CAACCAGCTG GCAGAGCTGG ACCGCCAGAT CAAGAGCTTC 
CGCGTGGCCT CCAACCCCTA CACCTGGTTC ACCATGGAGG CCCTGGAGGA GACCTGGAGA 
AACCTACAGA AGATCATCAA GGAAAGGGAG CTGGAGCTGC AGAAGGAGCA GCGACGGCAG 
GAGGAGAATG ACAAGCTGCG CCAGGAGTTC GCCCAGCATG CCAACGCCTT CCACCAGTGG 
ATCCAGGAGA CCAGAACGTA CCTCCTCGAT GGGTCCTGCA TGGTGGAAGA ATCCGGGACC 
CTCGAATCAC AGCTTGAAGC TACCAAACGC AAACACCAGG AGATTCGAGC TATGAGAAGT 
CAGCTCAAGA AGATTGAAGA CCTGGGGGCG GCCATGGAGG AAGCGCTGAT CCTGGACAAC 
AAGTACACGG AGCACAGCAC CGTGGGCCTG GCCCAGCAGT GGGACCAGCT CGACCAGTTG 
GGCATGCGCA TGCAGCACAA CCTGGAGCAG CAGATCCAGG CCAGGAACAC GACCGGAGTG 
ACCGAGGAGG CCCTCAAAGA ATTCAGCATG ATGTTCAAAC ACTTCGACAA GGACAAGTCT 
GGCCGGCTGA ACCACCAGGA GTTCAAATCC TGTCTGCGCT CCCTGGGCTA TGACCTGCCC 
ATGGTGGAGG AAGGGGAGCC CGACCCTGAG TTCGAGGCCA TCCTGGACAC TGTGGATCCT 
AACAGGGACG GCCACGTCTC CCTGCAAGAG TACATGGCCT TTATGATCAG CCGTGAGACC 
GAGAACGTCA AGTCCAGCGA GGAGATTGAG AGCGCCTTCC GGGCCCTGAG CTCTGAAGGG 
AAGCCTTATG TGACCAAGGA GGAGCTGTAC CAGAACCTGA CTCGGGAACA AGCCGACTAC 
TGCGTCTCCC ACATGAAGCC CTACGTGGAC GGCAAGGGCC GCGAACTCCC CACTGCTTTT 
GACTACGTCG AGTTCACCCG CTCGCTCTTC GTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010808764.1
CDS	26..7384
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLHQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMYRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVDHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWESLKSKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGAMPRDESDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKEKLDVLDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQSFADQLIAGGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQSKHQKHQAFEAELHANADRIRGVIDMGNSLIDRGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDAINGRFQKIKSMAASRRARLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLVKKNNHHEENISSKMKGLNGKVSDLEKAASQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHAALMKRWSQLLANSATRKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_010810462.2
Organism	Bos taurus(cattle)
Definition	Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010810462.2

ORF Insert Sequence:

ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT 
GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT 
CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC 
AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG 
AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC 
CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG 
AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT 
GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC 
CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC 
CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC 
CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT 
AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT 
GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG 
GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC 
ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC 
TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG 
AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG 
GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA 
GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG 
AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG 
CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG 
GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA 
ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG 
AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT 
GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT 
TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG 
CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT 
GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG 
AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG 
CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT 
CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC 
GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA 
GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG 
GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT 
CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG 
AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC 
AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC 
GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC 
CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC 
GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA 
GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC 
GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC 
AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC 
GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC 
AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGTACC ATTCTCTGCT GGAACTGGGA 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG 
AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC 
GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC 
AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA 
GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC 
AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG 
CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA 
GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC 
TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG 
GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC 
CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA 
TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC 
CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT 
CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG 
TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC 
GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG 
ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG 
GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG 
ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC 
GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG 
ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA 
GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG 
GACCCCACCA ACATCCAGAG CAAGCATCAG AAGCACCAGG CCTTCGAGGC AGAACTGCAC 
GCCAATGCGG ACCGGATCCG TGGGGTCATC GACATGGGCA ACTCCCTCAT TGACCGCGGG 
GCCTGCGCCG GCAGCGAGGA TGCCGTCAAG GCCCGGCTGG CCGCCTTAGC TGACCAGTGG 
CAGTTCCTGG TGCAGAAGTC GGCTGAGAAG AGCCAGAAGC TGAAAGAAGC CAACAAGCAG 
CAGAACTTCA ACACTGGCAT CAAGGACTTC GACTTCTGGC TCTCTGAGGT GGAAGCCCTG 
CTGGCCTCTG AGGATTATGG CAAAGACCTG GCTTCTGTGA ACAACTTGCT AAAAAAACAT 
CAGCTGCTGG AGGCAGATAT CTCTGCCCAC GAGGACCGCC TGAAGGACCT CAACAGCCAG 
GCGGACAGCC TGATGACCAG CAGCGCCTTC GACACCTCCC AGGTGAAGGA CAAGCGGGAC 
GCCATCAACG GCCGTTTCCA GAAGATCAAG AGCATGGCCG CCTCGAGGCG AGCTAGGCTG 
AATGAGTCCC ACCGCCTGCA CCAGTTTTTC CGGGACATGG ATGATGAGGA GTCCTGGATC 
AAGGAAAAGA AGCTGCTGGT GAGCTCAGAG GACTACGGCC GTGACCTGAC GGGCGTCCAG 
AACCTGAGGA AGAAGCACAA ACGGCTGGAA GCAGAGCTGG CTGCCCACGA GCCGGCCATT 
CAGGGTGTCC TGGACACTGG AAAGAAGCTG TCTGATGACA ACACCATCGG GAAGGAGGAG 
ATTCAGCAGC GCCTGGCGCA GTTCGTGGAG CACTGGAAGG AGCTGAAGCA GCTGGCAGCT 
GCCCGGGGCC AGCGGCTAGA AGAATCTTTG GAATATCAGC AGTTTGTAGC CAACGTGGAG 
GAGGAGGAAG CCTGGATCAA TGAGAAGATG ACCCTGGTGG CCAGTGAAGA TTATGGAGAC 
ACTCTTGCTG CCATCCAGGG CTTGCTGAAG AAACACGAAG CCTTCGAGAC GGACTTCACT 
GTCCACAAGG ACCGAGTGAA TGATGTCTGC ACCAACGGCC AGGACCTGGT TAAGAAGAAC 
AATCACCATG AGGAGAACAT CTCTTCCAAG ATGAAGGGCT TGAACGGGAA AGTGTCAGAC 
TTGGAGAAGG CTGCCTCCCA GAGGAAGGCC AAGCTCGACG AGAACTCGGC CTTCCTCCAG 
TTCAACTGGA AAGCCGATGT GGTGGAGTCC TGGATCGGTG AAAAGGAGAA CAGCTTGAAG 
ACAGATGATT ATGGTCGAGA TCTGTCTTCT GTCCAAACTC TCCTCACCAA ACAGGAAACC 
TTCGATGCTG GCCTGCAGGC CTTCCAGCAG GAGGGCATCG CCAACATCAC TGCCCTCAAA 
GATCAGCTGC TGGCCGCCAA GCACGTTCAG TCCAAGGCCA TCGAGGCCCG CCATGCTGCC 
CTCATGAAGA GGTGGAGCCA GCTCCTGGCC AACTCGGCCA CCCGGAAGAA GAAGCTGCTG 
GAGGCACAGA GTCACTTCCG CAAGGTGGAG GATCTCTTCC TGACCTTTGC TAAAAAGGCT 
TCAGCCTTCA ACAGCTGGTT TGAAAACGCA GAGGAGGACT TAACCGACCC AGTGCGCTGC 
AACTCTCTGG AAGAGATCAA GGCCTTGAGA GAGGCCCACG ACGCCTTCCG CTCCTCGTTG 
AGCTCGGCCC AGGCCGACTT CAACCAGCTG GCAGAGCTGG ACCGCCAGAT CAAGAGCTTC 
CGCGTGGCCT CCAACCCCTA CACCTGGTTC ACCATGGAGG CCCTGGAGGA GACCTGGAGA 
AACCTACAGA AGATCATCAA GGAAAGGGAG CTGGAGCTGC AGAAGGAGCA GCGACGGCAG 
GAGGAGAATG ACAAGCTGCG CCAGGAGTTC GCCCAGCATG CCAACGCCTT CCACCAGTGG 
ATCCAGGAGA CCAGAACGTA CCTCCTCGAT GGGTCCTGCA TGGTGGAAGA ATCCGGGACC 
CTCGAATCAC AGCTTGAAGC TACCAAACGC AAACACCAGG AGATTCGAGC TATGAGAAGT 
CAGCTCAAGA AGATTGAAGA CCTGGGGGCG GCCATGGAGG AAGCGCTGAT CCTGGACAAC 
AAGTACACGG AGCACAGCAC CGTGGGCCTG GCCCAGCAGT GGGACCAGCT CGACCAGTTG 
GGCATGCGCA TGCAGCACAA CCTGGAGCAG CAGATCCAGG CCAGGAACAC GACCGGAGTG 
ACCGAGGAGG CCCTCAAAGA ATTCAGCATG ATGTTCAAAC ACTTCGACAA GGACAAGTCT 
GGCCGGCTGA ACCACCAGGA GTTCAAATCC TGTCTGCGCT CCCTGGGCTA TGACCTGCCC 
ATGGTGGAGG AAGGGGAGCC CGACCCTGAG TTCGAGGCCA TCCTGGACAC TGTGGATCCT 
AACAGGGACG GCCACGTCTC CCTGCAAGAG TACATGGCCT TTATGATCAG CCGTGAGACC 
GAGAACGTCA AGTCCAGCGA GGAGATTGAG AGCGCCTTCC GGGCCCTGAG CTCTGAAGGG 
AAGCCTTATG TGACCAAGGA GGAGCTGTAC CAGAACCTGA CTCGGGAACA AGCCGACTAC 
TGCGTCTCCC ACATGAAGCC CTACGTGGAC GGCAAGGGCC GCGAACTCCC CACTGCTTTT 
GACTACGTCG AGTTCACCCG CTCGCTCTTC GTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_024999042.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854810.1 Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OBa54554
Clone ID Related Accession (Same CDS sequence)	XM_010810462.2 , XM_024999042.1
Accession Version	XM_024999042.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT 
GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT 
CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC 
AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG 
AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC 
CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG 
AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT 
GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC 
CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC 
CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC 
CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT 
AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT 
GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG 
GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC 
ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC 
TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG 
AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG 
GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA 
GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG 
AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG 
CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG 
GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA 
ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG 
AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT 
GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT 
TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG 
CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT 
GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG 
AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG 
CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT 
CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC 
GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA 
GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG 
GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT 
CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG 
AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC 
AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC 
GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC 
CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC 
GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA 
GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC 
GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC 
AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC 
GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC 
AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGTACC ATTCTCTGCT GGAACTGGGA 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG 
AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC 
GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC 
AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA 
GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC 
AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG 
CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA 
GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC 
TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG 
GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC 
CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA 
TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC 
CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT 
CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG 
TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC 
GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG 
ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG 
GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG 
ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC 
GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG 
ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA 
GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG 
GACCCCACCA ACATCCAGAG CAAGCATCAG AAGCACCAGG CCTTCGAGGC AGAACTGCAC 
GCCAATGCGG ACCGGATCCG TGGGGTCATC GACATGGGCA ACTCCCTCAT TGACCGCGGG 
GCCTGCGCCG GCAGCGAGGA TGCCGTCAAG GCCCGGCTGG CCGCCTTAGC TGACCAGTGG 
CAGTTCCTGG TGCAGAAGTC GGCTGAGAAG AGCCAGAAGC TGAAAGAAGC CAACAAGCAG 
CAGAACTTCA ACACTGGCAT CAAGGACTTC GACTTCTGGC TCTCTGAGGT GGAAGCCCTG 
CTGGCCTCTG AGGATTATGG CAAAGACCTG GCTTCTGTGA ACAACTTGCT AAAAAAACAT 
CAGCTGCTGG AGGCAGATAT CTCTGCCCAC GAGGACCGCC TGAAGGACCT CAACAGCCAG 
GCGGACAGCC TGATGACCAG CAGCGCCTTC GACACCTCCC AGGTGAAGGA CAAGCGGGAC 
GCCATCAACG GCCGTTTCCA GAAGATCAAG AGCATGGCCG CCTCGAGGCG AGCTAGGCTG 
AATGAGTCCC ACCGCCTGCA CCAGTTTTTC CGGGACATGG ATGATGAGGA GTCCTGGATC 
AAGGAAAAGA AGCTGCTGGT GAGCTCAGAG GACTACGGCC GTGACCTGAC GGGCGTCCAG 
AACCTGAGGA AGAAGCACAA ACGGCTGGAA GCAGAGCTGG CTGCCCACGA GCCGGCCATT 
CAGGGTGTCC TGGACACTGG AAAGAAGCTG TCTGATGACA ACACCATCGG GAAGGAGGAG 
ATTCAGCAGC GCCTGGCGCA GTTCGTGGAG CACTGGAAGG AGCTGAAGCA GCTGGCAGCT 
GCCCGGGGCC AGCGGCTAGA AGAATCTTTG GAATATCAGC AGTTTGTAGC CAACGTGGAG 
GAGGAGGAAG CCTGGATCAA TGAGAAGATG ACCCTGGTGG CCAGTGAAGA TTATGGAGAC 
ACTCTTGCTG CCATCCAGGG CTTGCTGAAG AAACACGAAG CCTTCGAGAC GGACTTCACT 
GTCCACAAGG ACCGAGTGAA TGATGTCTGC ACCAACGGCC AGGACCTGGT TAAGAAGAAC 
AATCACCATG AGGAGAACAT CTCTTCCAAG ATGAAGGGCT TGAACGGGAA AGTGTCAGAC 
TTGGAGAAGG CTGCCTCCCA GAGGAAGGCC AAGCTCGACG AGAACTCGGC CTTCCTCCAG 
TTCAACTGGA AAGCCGATGT GGTGGAGTCC TGGATCGGTG AAAAGGAGAA CAGCTTGAAG 
ACAGATGATT ATGGTCGAGA TCTGTCTTCT GTCCAAACTC TCCTCACCAA ACAGGAAACC 
TTCGATGCTG GCCTGCAGGC CTTCCAGCAG GAGGGCATCG CCAACATCAC TGCCCTCAAA 
GATCAGCTGC TGGCCGCCAA GCACGTTCAG TCCAAGGCCA TCGAGGCCCG CCATGCTGCC 
CTCATGAAGA GGTGGAGCCA GCTCCTGGCC AACTCGGCCA CCCGGAAGAA GAAGCTGCTG 
GAGGCACAGA GTCACTTCCG CAAGGTGGAG GATCTCTTCC TGACCTTTGC TAAAAAGGCT 
TCAGCCTTCA ACAGCTGGTT TGAAAACGCA GAGGAGGACT TAACCGACCC AGTGCGCTGC 
AACTCTCTGG AAGAGATCAA GGCCTTGAGA GAGGCCCACG ACGCCTTCCG CTCCTCGTTG 
AGCTCGGCCC AGGCCGACTT CAACCAGCTG GCAGAGCTGG ACCGCCAGAT CAAGAGCTTC 
CGCGTGGCCT CCAACCCCTA CACCTGGTTC ACCATGGAGG CCCTGGAGGA GACCTGGAGA 
AACCTACAGA AGATCATCAA GGAAAGGGAG CTGGAGCTGC AGAAGGAGCA GCGACGGCAG 
GAGGAGAATG ACAAGCTGCG CCAGGAGTTC GCCCAGCATG CCAACGCCTT CCACCAGTGG 
ATCCAGGAGA CCAGAACGTA CCTCCTCGAT GGGTCCTGCA TGGTGGAAGA ATCCGGGACC 
CTCGAATCAC AGCTTGAAGC TACCAAACGC AAACACCAGG AGATTCGAGC TATGAGAAGT 
CAGCTCAAGA AGATTGAAGA CCTGGGGGCG GCCATGGAGG AAGCGCTGAT CCTGGACAAC 
AAGTACACGG AGCACAGCAC CGTGGGCCTG GCCCAGCAGT GGGACCAGCT CGACCAGTTG 
GGCATGCGCA TGCAGCACAA CCTGGAGCAG CAGATCCAGG CCAGGAACAC GACCGGAGTG 
ACCGAGGAGG CCCTCAAAGA ATTCAGCATG ATGTTCAAAC ACTTCGACAA GGACAAGTCT 
GGCCGGCTGA ACCACCAGGA GTTCAAATCC TGTCTGCGCT CCCTGGGCTA TGACCTGCCC 
ATGGTGGAGG AAGGGGAGCC CGACCCTGAG TTCGAGGCCA TCCTGGACAC TGTGGATCCT 
AACAGGGACG GCCACGTCTC CCTGCAAGAG TACATGGCCT TTATGATCAG CCGTGAGACC 
GAGAACGTCA AGTCCAGCGA GGAGATTGAG AGCGCCTTCC GGGCCCTGAG CTCTGAAGGG 
AAGCCTTATG TGACCAAGGA GGAGCTGTAC CAGAACCTGA CTCGGGAACA AGCCGACTAC 
TGCGTCTCCC ACATGAAGCC CTACGTGGAC GGCAAGGGCC GCGAACTCCC CACTGCTTTT 
GACTACGTCG AGTTCACCCG CTCGCTCTTC GTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854810.1
CDS	120..7478
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLHQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMYRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVDHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWESLKSKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGAMPRDESDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKEKLDVLDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQSFADQLIAGGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQSKHQKHQAFEAELHANADRIRGVIDMGNSLIDRGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDAINGRFQKIKSMAASRRARLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLVKKNNHHEENISSKMKGLNGKVSDLEKAASQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHAALMKRWSQLLANSATRKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_024999042.1
Organism	Bos taurus(cattle)
Definition	Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999042.1

ORF Insert Sequence:

ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT 
GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT 
CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC 
AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG 
AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC 
CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG 
AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT 
GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC 
CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC 
CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC 
CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT 
AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT 
GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG 
GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC 
ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC 
TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG 
AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG 
GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA 
GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG 
AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG 
CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG 
GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA 
ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG 
AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT 
GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT 
TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG 
CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT 
GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG 
AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG 
CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT 
CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC 
GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA 
GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG 
GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT 
CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG 
AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC 
AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC 
GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC 
CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC 
GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA 
GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC 
GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC 
AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC 
GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC 
AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGTACC ATTCTCTGCT GGAACTGGGA 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG 
AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC 
GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC 
AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA 
GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC 
AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG 
CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA 
GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC 
TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG 
GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC 
CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA 
TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC 
CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT 
CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG 
TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC 
GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG 
ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG 
GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG 
ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC 
GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG 
ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA 
GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG 
GACCCCACCA ACATCCAGAG CAAGCATCAG AAGCACCAGG CCTTCGAGGC AGAACTGCAC 
GCCAATGCGG ACCGGATCCG TGGGGTCATC GACATGGGCA ACTCCCTCAT TGACCGCGGG 
GCCTGCGCCG GCAGCGAGGA TGCCGTCAAG GCCCGGCTGG CCGCCTTAGC TGACCAGTGG 
CAGTTCCTGG TGCAGAAGTC GGCTGAGAAG AGCCAGAAGC TGAAAGAAGC CAACAAGCAG 
CAGAACTTCA ACACTGGCAT CAAGGACTTC GACTTCTGGC TCTCTGAGGT GGAAGCCCTG 
CTGGCCTCTG AGGATTATGG CAAAGACCTG GCTTCTGTGA ACAACTTGCT AAAAAAACAT 
CAGCTGCTGG AGGCAGATAT CTCTGCCCAC GAGGACCGCC TGAAGGACCT CAACAGCCAG 
GCGGACAGCC TGATGACCAG CAGCGCCTTC GACACCTCCC AGGTGAAGGA CAAGCGGGAC 
GCCATCAACG GCCGTTTCCA GAAGATCAAG AGCATGGCCG CCTCGAGGCG AGCTAGGCTG 
AATGAGTCCC ACCGCCTGCA CCAGTTTTTC CGGGACATGG ATGATGAGGA GTCCTGGATC 
AAGGAAAAGA AGCTGCTGGT GAGCTCAGAG GACTACGGCC GTGACCTGAC GGGCGTCCAG 
AACCTGAGGA AGAAGCACAA ACGGCTGGAA GCAGAGCTGG CTGCCCACGA GCCGGCCATT 
CAGGGTGTCC TGGACACTGG AAAGAAGCTG TCTGATGACA ACACCATCGG GAAGGAGGAG 
ATTCAGCAGC GCCTGGCGCA GTTCGTGGAG CACTGGAAGG AGCTGAAGCA GCTGGCAGCT 
GCCCGGGGCC AGCGGCTAGA AGAATCTTTG GAATATCAGC AGTTTGTAGC CAACGTGGAG 
GAGGAGGAAG CCTGGATCAA TGAGAAGATG ACCCTGGTGG CCAGTGAAGA TTATGGAGAC 
ACTCTTGCTG CCATCCAGGG CTTGCTGAAG AAACACGAAG CCTTCGAGAC GGACTTCACT 
GTCCACAAGG ACCGAGTGAA TGATGTCTGC ACCAACGGCC AGGACCTGGT TAAGAAGAAC 
AATCACCATG AGGAGAACAT CTCTTCCAAG ATGAAGGGCT TGAACGGGAA AGTGTCAGAC 
TTGGAGAAGG CTGCCTCCCA GAGGAAGGCC AAGCTCGACG AGAACTCGGC CTTCCTCCAG 
TTCAACTGGA AAGCCGATGT GGTGGAGTCC TGGATCGGTG AAAAGGAGAA CAGCTTGAAG 
ACAGATGATT ATGGTCGAGA TCTGTCTTCT GTCCAAACTC TCCTCACCAA ACAGGAAACC 
TTCGATGCTG GCCTGCAGGC CTTCCAGCAG GAGGGCATCG CCAACATCAC TGCCCTCAAA 
GATCAGCTGC TGGCCGCCAA GCACGTTCAG TCCAAGGCCA TCGAGGCCCG CCATGCTGCC 
CTCATGAAGA GGTGGAGCCA GCTCCTGGCC AACTCGGCCA CCCGGAAGAA GAAGCTGCTG 
GAGGCACAGA GTCACTTCCG CAAGGTGGAG GATCTCTTCC TGACCTTTGC TAAAAAGGCT 
TCAGCCTTCA ACAGCTGGTT TGAAAACGCA GAGGAGGACT TAACCGACCC AGTGCGCTGC 
AACTCTCTGG AAGAGATCAA GGCCTTGAGA GAGGCCCACG ACGCCTTCCG CTCCTCGTTG 
AGCTCGGCCC AGGCCGACTT CAACCAGCTG GCAGAGCTGG ACCGCCAGAT CAAGAGCTTC 
CGCGTGGCCT CCAACCCCTA CACCTGGTTC ACCATGGAGG CCCTGGAGGA GACCTGGAGA 
AACCTACAGA AGATCATCAA GGAAAGGGAG CTGGAGCTGC AGAAGGAGCA GCGACGGCAG 
GAGGAGAATG ACAAGCTGCG CCAGGAGTTC GCCCAGCATG CCAACGCCTT CCACCAGTGG 
ATCCAGGAGA CCAGAACGTA CCTCCTCGAT GGGTCCTGCA TGGTGGAAGA ATCCGGGACC 
CTCGAATCAC AGCTTGAAGC TACCAAACGC AAACACCAGG AGATTCGAGC TATGAGAAGT 
CAGCTCAAGA AGATTGAAGA CCTGGGGGCG GCCATGGAGG AAGCGCTGAT CCTGGACAAC 
AAGTACACGG AGCACAGCAC CGTGGGCCTG GCCCAGCAGT GGGACCAGCT CGACCAGTTG 
GGCATGCGCA TGCAGCACAA CCTGGAGCAG CAGATCCAGG CCAGGAACAC GACCGGAGTG 
ACCGAGGAGG CCCTCAAAGA ATTCAGCATG ATGTTCAAAC ACTTCGACAA GGACAAGTCT 
GGCCGGCTGA ACCACCAGGA GTTCAAATCC TGTCTGCGCT CCCTGGGCTA TGACCTGCCC 
ATGGTGGAGG AAGGGGAGCC CGACCCTGAG TTCGAGGCCA TCCTGGACAC TGTGGATCCT 
AACAGGGACG GCCACGTCTC CCTGCAAGAG TACATGGCCT TTATGATCAG CCGTGAGACC 
GAGAACGTCA AGTCCAGCGA GGAGATTGAG AGCGCCTTCC GGGCCCTGAG CTCTGAAGGG 
AAGCCTTATG TGACCAAGGA GGAGCTGTAC CAGAACCTGA CTCGGGAACA AGCCGACTAC 
TGCGTCTCCC ACATGAAGCC CTACGTGGAC GGCAAGGGCC GCGAACTCCC CACTGCTTTT 
GACTACGTCG AGTTCACCCG CTCGCTCTTC GTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	SPTAN1
Entrez Gene ID	540108
Full Name	spectrin alpha, non-erythrocytic 1
General protein information	Preferred Names spectrin alpha, non-erythrocytic 1 Names spectrin alpha chain, non-erythrocytic 1 alpha-fodrin
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Rattus norvegicus (Norway rat)	Sptan1	NP_741984.2
Danio rerio (zebrafish)	spna2	NP_001091958.1
Caenorhabditis elegans (roundworm)	spc-1	NP_001257000.1
Xenopus tropicalis (tropical clawed frog)	sptan1	NP_001090674.1
Canis lupus familiaris (dog)	SPTAN1	XP_005625341.1
Bos taurus (cattle)	SPTAN1	NP_001107628.1
Mus musculus (house mouse)	Sptan1	NP_001171138.1
Gallus gallus (chicken)	SPTAN1	NP_001036003.1
Homo sapiens (human)	SPTAN1	NP_001123910.1
	SPTAN1	XP_002800011.1
Drosophila melanogaster (fruit fly)	alpha-Spec	NP_001261286.1
Pan troglodytes (chimpanzee)	SPTAN1	XP_003312345.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006686	XP_316724.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

SPTAN1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence: