spc-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	spc-1
Entrez Gene ID	180603
Full Name	SPeCtrin
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_001270071.3	NP_001257000.1	SPeCtrin
NM_001270070.2	NP_001256999.1	SPeCtrin
NM_001270070.1	NP_001256999.1	SPeCtrin
NM_001270071.1	NP_001257000.1	SPeCtrin

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Sptan1	NP_741984.2
Danio rerio (zebrafish)	spna2	NP_001091958.1
Caenorhabditis elegans (roundworm)	spc-1	NP_001257000.1
Xenopus tropicalis (tropical clawed frog)	sptan1	NP_001090674.1
Canis lupus familiaris (dog)	SPTAN1	XP_005625341.1
Bos taurus (cattle)	SPTAN1	NP_001107628.1
Mus musculus (house mouse)	Sptan1	NP_001171138.1
Gallus gallus (chicken)	SPTAN1	NP_001036003.1
Homo sapiens (human)	SPTAN1	NP_001123910.1
	SPTAN1	XP_002800011.1
Drosophila melanogaster (fruit fly)	alpha-Spec	NP_001261286.1
Pan troglodytes (chimpanzee)	SPTAN1	XP_003312345.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006686	XP_316724.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following spc-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the spc-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa349360	NM_001270070.2 Latest version!	Caenorhabditis elegans SPeCtrin (spc-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OCa349365	NM_001270071.3 Latest version!	Caenorhabditis elegans SPeCtrin (spc-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
View Old Accession Versions >>
OCa349360	NM_001270070.1	Caenorhabditis elegans SPeCtrin (spc-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OCa349365	NM_001270071.1	Caenorhabditis elegans SPeCtrin (spc-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

spc-1 ( NM_001270070.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001256999.1 Caenorhabditis elegans SPeCtrin (spc-1), mRNA.

Price & Availability↑

CloneID	OCa349360
Clone ID Related Accession (Same CDS sequence)	NM_001270070.2 , NM_001270070.1
Accession Version	NM_001270070.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7284bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	SPeCtrin
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Sep 4, 2019 this sequence version replaced NM_001270070.1.

ATGGCTGATT CGAATGACAC TCATGCTCCA CCAGAGCCGG TGCTAGAAGT ACCGCCACCA 
CAGGAGATTC GTATTCTGGA GACTGCTGAT GACATTCACC AACGTCGTCA GGAAGTATTA 
GGTCACTATT CAATTTTCAA GGATCACTCG CAGACAAAAC GAGATCGTCT CGAGGAAGCT 
CGTCAGTTTC AATATTTCAA ACGAGATGCT GATGAGTTGA ACGTTTGGAT TCTTGAAAAG 
CTCCAGACAG CTCAGGAAGA AAACTTCAGA GATCCGAGCA ATCTCCAGGC CAAGATCCAG 
AAGCATGAAG CGTTCGAAGC TGAGGTTCAA GCTCACGCTA AGACCATCGC CAACCTCGAC 
AAGACCGGAA ACGCTATGAT CCAACACAAT CATTTCGCTA GCGAACACAT CAAGAAACGC 
TTGGAAGAGT TGCACGCCCT CTGGGATAAG CTCTTCTTCA AACTGAAGGA TAAGGGAATT 
AAGCTACAAC AAGCACTTAA ACTTTTGCAT TTTATTCGTC AATGCGATGA AATTCTCTAC 
TGGATTCGAG AGAAGGAAAC CTATGTCACC GCAGAAGACA TGGGAATGGA TCTCGAACAC 
GTTGAGATAC TTCAGCGTAA ATTTGACGAC TTTTTGAAAG AACTCGGTAA CCATCAGTAC 
CGAATCAATG AGATCAATCA AGCTGCTGAC AAACTTGTCG AGGAGGGACA CTCTGAGCAC 
GATCAGATCT ACAAGAAGCG CGATGACGTT AATGATGCTT GGCATCGTTT GAACACTTTG 
GCGGCCACTC GTAAGGAAGG GCTCTTTGGA GCTCATCAAG TTCAACGCTT CAATCGCGAT 
GCAGACGAGA CCCTTGCATG GATTGGAGAG AAGGATCTTG CTTTGTCAAG CGATGATTAC 
GGAAGAGACC TCAATAATGT TCAAGCTCTT CAACGCAAGC ATGAAGGAAC TGAACGTGAT 
CTTGCTGCTC TTGAAGGAAA GATGCTCCAA TTGGAGAAAG AAGCTCATAA GTTGGCAGAA 
ACTCATCCAG ATCGTGCTGA AGCGATTCAT CAAAAGAACG ATGACACCAA AGCCGCCTGG 
AATGCACTCA AGGAGAAGGC TCAACACCGC AAAGATGGAC TTGAGAGAAG CTTCCAGCTT 
CATCGTTTCT TGGCCGACTA CCGTGACTTG ATTAGCTGGA TTGGAGACAT GAAAGCTGTC 
ATCGGAGCTG ATGAGCTTGC AAAAGACGTG GCTGGCGCTG AAGCTCTTCT TGAAAGTCAT 
CAAGAACACA AGGGAGAAAT TGATGCTCGT GCTGACTCGT TCAACCAAAC TGCATCCGCT 
GGACAAAAGC TCGTTGAGAT GGGAATTCCA GAGAGTCCAG AAGTTCAGGA GAAGCTCGAG 
AAACTTGACC ACGAGAAGTC TTCTCTTCTT GGATTGTGGG AGGAACGTCG TATTCTCTAC 
GAGCAATGTA TGGATCTCCA ACTCTTCTAT CGTGACACTG AACAAGCCGA AACCTGGATG 
AACAAGCAGG AAGCGTTCCT TGCCAACACT GATTTGGGAG ATTCACTAGA TTCTGTCGAG 
CACCTCATCA AGAAACACGA GGACTTTGAG AAATCGCTTG CTGCTCAAGA GGAGAAGATC 
AATGCACTTG ACGAGTTTGC TACCAAGCTC ATTCAAGGAC AGCATTACGC TGCTGATGAT 
GTTGCCGAGA GACGCCAAGC TCTTCTTGAC CGTCGTCGTC GTCTTCTAGA CAGAGCTCGT 
CAACGTGGAA ATGCTCTCAA GGAAAGCTAC AAGCGCCAGA CATTTGACCG TGACTGTGAT 
GAGATGGTTA GCTGGATCAC TGAAAAACTG TCCACCGCTC GTGACGATTC TTACTTGGAT 
CCTACCAACA TTCGTGGAAA ACTCCAAAAG CACATCAACT TTGAGCAAGA GCTTAAGGCT 
AACGAGAACC GTTTGGACGA TATTCGTTCT ACCGGAGAGC AAATCATCGA TAGTGGACAC 
TTTGCTGCTG ATCATATCGG AGACCGCCTT CGTCAAGTCA ACAACTTGTG GAACGAATTG 
GTCGATGCTA CAAACAAGAA GGGAGCCAAA CTTCGTGAAG CTGGAAACGA ACAGCAATTC 
AATCGCAACA TTGAAGATGT TGAAGCATGG TTGTCGGAGC TTGAAGGACA AGTTGCTTCC 
GAGGATTATG GAAAAGATCT TGTATCTGTT CAAAACCTTC AGAAGAAGAT TGGACTTATT 
GAGTCTGATT TTAACGCTCA CAACGATCGT GTTGATGGAA TCAAGCAACT TGCTCAACAG 
TTCCAAGAGG AGGAGCATTT CAATGCTCCA GTCATTGTTC GCAAGCAAGA GAATCTTCAG 
CAGCGCTACA ACGCTCTCCG TGATCCACTT GAGAAGAGAA AAAGAAAACT CGGAGAGTCC 
CACCAAGGAA ATCAACTGTT CCGCGATATT GAAGATGAGC TGGCTTGGAT CCGTGAGAAG 
GAACAAGTTG CTGGATCAAC CAATCGAGGA CGTGATTTGA TTGGAGTTCA AAATCTCATC 
AAGAAGCAGC AGGCTCTGAT CGCTGAGATT GCCAACCACG ATAGCCAAAT CGAGAGTGTC 
AGCAGTGCCG CCAATGACAT GATTCAACAA GGACACTTCT TGGCTCCAGA GATACGTGAT 
AAACTTGCTC AACTTCGTGA CAACTGGAGA ATTCTGAAGA GCAAGGCTGA GAAGAGACGC 
ACAGATCTTG ATGACTCACT CCAAGCCCAC CAATACCTTT CTGATGCCAA TGAGGCCGAT 
GCTTGGATGA GCGAGAAAGA ACCAATTGTT GGATCCACCG ACTATGGAAA GGACGAAGAT 
TCTGCCGAGG CTCTTCTCAA GAAGCATCGT GCTCTTCTTT CCGACTTGGA AGCTTTCAAG 
GGAACTATCG AGGATCTTCG CAAGCAAGCT TCTCAATGCA AGTACCAAGA GCAACCGATG 
GGACAACTCG GACGTGACTG TGTGTTGGCT CTTTACGATT ACCAAGAGAA ATCTCCTCGT 
GAGGTTTCCA TGAAGAAGGG AGATGTTCTC ACTCTTCTCA ATGCTAGCAA CAGAGACTGG 
TGGAAAGTTG AGGTCAATGA TCGTCAAGGA TTTGTTCCAG CTGCCTACGT TAAGAGAATC 
GAACCAGGAA CTGCTCAACA GCATGCTCAA CAACAGGTCA ACTCCATTGG AGGAAAGCAG 
TCGGAGATTG AGGACAAGTA CCAACGTCTC ATGATGCTCG GAGAGACTAG AAAGAGAAAG 
CTCGAGGAAG CTTGCAAAGG ATACCAACTT CTCCGTGAAG CCAACGACTT GGCTGAATGG 
ATCAAGTCTA GAGAGGCTGT TGCTGCTCAA CAAGAAATTG GAACTGACCT CGAGCAGGTT 
GAGGTTCTTC AAAAGAAGTT TGACGACTTT AAAGGAGACC TCAAGGCTAA CGAGGTTCGT 
CTCCAGGAGA TGAACCAAAT TGCCACCGCT CTCACATCGG TTGGACAAAC TGAAACTGCT 
GTTCGTATTC GTCAGCAAAT TGAGGATCTC AACGCCAGAT GGCGCGCATT GGAGGAGCAG 
ACTGAGCAAC GCGAACAACA ACTCGGATCT GCCCACGAGG TGCAACGCTT CCACCGTGAT 
GTCGACGAGA CTCGTGATTG GATCCAGGAG AAGGATGATG CCCTTGACAG TGAAGATTTT 
GGACGTGACC TCCGTTCCGT TCAGGCTCTT CAACGCAAGC ATGAAGGAGT TGAGCGTGAT 
TTGGCTGCTC TTGGAGACAA GATTAAATCT CTTGATGAGA AGGCCAACCG TCTTCGTCAA 
TCTCACCCAG AAGCTGCCGA GCAGATTTAC GATTTGCAAC GTGAGCTCAA TGAGCAATGG 
AACCGCCTCA CGTCTAAGGC CAACAACCGA AAAGAAAAGC TTCTTGATTC GTACGACTAC 
CAACGCTTTT TGTCTGACTA CCGTGATTTG ATGCAATGGA TTGCTTCCAT GAACCAACTT 
GTCAGCAGTC AAGAACTTGC CAACGATGTT ACTGGAGCTG AGGCTCTTCT TGAGCGCCAC 
CAGGAGTACC GCACCGAAAT TGACTCCCGT GCTGCCACCT TCCAAGCTTT TGATCAATTT 
GGAAACCAAC TTCTTAACAG CCACCACTAC GCTGCAGGAG ACATTGAAAA CCGTCTTCAA 
GGTGTCAACG AGGCTCGTAA AGGACTTGAA GACGCATGGG TTGCTCGTCG TAACATTCTC 
GATCAATGCC TCGAACTTCA ACTCTTCTAC CGTGATTGTG AGCAAGCCGA CACTTGGATG 
TCAGCTCGTG AAGCTTTCTT GGCTCAAGAG GATCCATCTG GAGACAATGT TGAGTCTTTG 
ATCAAGAAAC ACGAGGACTT TGACAAAGCC ATCAACACTC AAGAGGAGAA AATCAAGGGA 
CTCAAGCTCT TTGCTGAATC TCTCATCAAG AACAATCATT ACGATTCGCC AGCAGTCACT 
CGCAAGAGAG ACCAGATTCT CGATAGATGG AATGGTCTTA AGGAGGCTCT CATTCAGAAG 
AGATTCAAGC TTGGAGAGTC ACAAACTCTT CAACAGTTTA GTCGTGATGC GGATGAGATT 
GAGAACTGGA TGACTGAGAA GTTCCAGATT GCTCAAGAGG AGAACTACCG CGATCCAACC 
AACATTCAGC AGAAACATCA GAAGCAACAA GCCTTCGAAG CCGAGCTTCA TGCCAATTCG 
GACAGAATCG CTGCTATCAT TCAAGCTGGA AACAACCTCA TTGAGAACGC CAAGTGCGGA 
GGAGGTGAAG CTGCTGTCAG CGCTCGACTG AAGGCTCTCA ACGACCAATG GGACCTTCTT 
GTCAAGACCA CCACCGAGAA GAGTTACCGT TTGAAAGAGG CTAACAAGCA GAAGAGCTTC 
ATGGCTGCTG TCAAGGATCT TGAGTTCTGG CTGGGAGAGG TTGAAATTCT CCTCCAATCT 
GAAGACTATG GAAAAGATTT GGCTTCCGTT GAAAACCTGC TCAAAAAACA TTCTCTTCTT 
GAGGCTGACA TTGTTGCCCA CCAGGATCGT GTTGGAGAGA TGAACGAGCA AGCTGATTCT 
CTTCTCGAGA ACGATCAATT CCAAGGACAA CAAATCGCTG AGAGAAGAAA GTTGATTGCT 
GATCGTTATG ATGGTGTCAA GAAGATGGCT ACCGATCGTC GTGATAAATT GAGCAAGGCC 
CTCAATGTTC ACCAGTTCTT CCGTGATATT GATGATGAGG AGTCTTGGAT TAAGGAGAAG 
AAGCTTCTCG TTTCTTCTGA TGACTATGGA CGTGATCTTC CAGGAGTTCA AAACCTTCGC 
CGCAAGCATC GTCGTATTGA TACCGAGCTC GCTTCCCATG AGCCTCAAGT TTCTCTTGTC 
AAGCTGAAGG GAGAAGAGCT GTTGAGATCT GCAGCCGAAG CGGGAGTTGG AGAGGATCAA 
ATTAAGAAGC GTATGGAGGA TCTTGAGCAA AGCTGGGGAC AAATCCGTGA TCTTACTGGA 
AACCGTCATC AACGCCTCGA CGAGTCTGAA GCCTTCCAAG CTTTCTTGGG TGATGTTGAA 
GAGGAAGAGG CATGGATGAA TGAGAAACAA CAAATCCTGG GATCCGACAA CTTTGGAGAA 
AACATGGCTG GAGTGCAAGG ACTTCTCAAG AAGCACGATA CGTTCCAAGT CGATCTTGAG 
CTTCACAAGC AACGTGTTGC TGATTTGATC AACAAGGGAG ACACTCTTAT TCACAATGGA 
AACCATCATG CTCCTCATAT CAAGCAGAGA TGCGACCAAC TCCGTGCTCG TCTTATTGAG 
ATTGAGAGCA TGGCCGAAGG ACGTCTTGCC AAGCTTCGTG ATAACTCTGC CTACCTTCAA 
TTCATGTGGA AGTGTGATGT TGTGGAATCG TGGATTGCTG AGAAGGAGCA ACAAGTTCGT 
TCCGAAGACT TTGGAAGAGA TCTCTCATCT GTTCAAATCC TCCTCACCAA GCAAGAAGCT 
TTCGACGCTG GACTCAACGC TTTCGAACAT GAAGGAATTC AAAGAATCAC CGAGCTCAAA 
GACCAACTTG TTTCGAGTGA GCACCAACAA TCTCCAGCCA TTGAGAAACG TCATACGAAC 
GTCATCCAAC GTTGGCAGAA CCTTCTTGCC CACTCCGAGG CTCGTCGTCA GAAGTTGTTG 
AAAATGCAAC AGCAATTCAA GCAAATCGAG GAGCTTTACC TTGCCTTTGC CAAGAAGGCT 
TCCACTTTCA ACTCATGGTT CGAGAACGCT GAGGAGGACT TGACTGACCC TGTCCGATGC 
AACTCTTTGG AAGAGATCCG TGCCTTGCGC GACGCCCATG GTGAGTTCCA GCGCTCATTG 
TCCTCCGCTG AAGAGGACTT CCGCCAACTC CAAGACCTTG ATCGTCGGAT CAAGTCGTTC 
AATGTGGGAC CCAACCCTTA CACGTGGTTC ACTATGGATG CCCTCGAAGA CACGTGGCGC 
AACCTGCAGA GAATCATCAA GGAGCGCGAG CAGGAGCTTG CCAGAGAGCA CCAAAGACAG 
GAGGAGAACG ACAAATTGCG ACGCGAGTTT GCCAAGTTGG CCAATGCTTT CCATGCTTGG 
TTGACAAATA CCAGACAGGA GATGATGGAA GCTGGAGGTA CATTGGAAGA ACAACTCGAT 
GCTGTTGAGA GGAAAGCAAA GGAAATCAAG GCGAACAAAG GTCAGCTGCG GCAGATTGAA 
GAGAAGGGTG CTATGTTGGA GAGAAACCTT ATCCTAGACA ATAGGTATAC TGAACATTCG 
ACAGTAGGAA TTGCGCAAGC TTGGGATCAA TTGGATCAGC TAGCCATGCG AATGCAACAC 
AATTTGGAAC AACAGATTCA AGCCAGAAAT CAATCTGGAG TATCGGAAGA AGCCCTTCGT 
GAGTTCTCAA TGATGTTCAA GCACTTCGAC AAGGAGAAAA CTGGAAGACT CGACCACCAG 
CAGTTCAAGA GTTGTCTGCG AGCTCTTGGA TACGATCTGC CGATGGTGGA CGAAGGCCAA 
CCAGAACCCG AGTTCCAGAG AATTTTGGAT ATTGTCGACC CGAACAGAGA TGGTTACGTA 
ACTCTGCAAG AGTACATGGC GTTCATGATC AGCAAAGAAA CCGAGAACAT CCAGTCTTCG 
GAAGAAATCG AGATGGCATT CAGAGCTCTT TCGAAGGAAT TCCGACCATA TGTCACGGCA 
GAGGAGCTCT ACGCGAACCT GACACCGGAG CAAGCCGAAT TCTGCATACG AAGAATGAAG 
CCATACATGG ACGCCATTTC AGGTCGTTCC ATCCAAGGTG GTCTCGACTA CGAGCAGTTT 
GTGCATGCCT TGTTCCAGAG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001256999.1
CDS	13..7296
Translation	MADSNDTHAPPEPVLEVPPPQEIRILETADDIHQRRQEVLGHYSIFKDHSQTKRDRLEEARQFQYFKRDADELNVWILEKLQTAQEENFRDPSNLQAKIQKHEAFEAEVQAHAKTIANLDKTGNAMIQHNHFASEHIKKRLEELHALWDKLFFKLKDKGIKLQQALKLLHFIRQCDEILYWIREKETYVTAEDMGMDLEHVEILQRKFDDFLKELGNHQYRINEINQAADKLVEEGHSEHDQIYKKRDDVNDAWHRLNTLAATRKEGLFGAHQVQRFNRDADETLAWIGEKDLALSSDDYGRDLNNVQALQRKHEGTERDLAALEGKMLQLEKEAHKLAETHPDRAEAIHQKNDDTKAAWNALKEKAQHRKDGLERSFQLHRFLADYRDLISWIGDMKAVIGADELAKDVAGAEALLESHQEHKGEIDARADSFNQTASAGQKLVEMGIPESPEVQEKLEKLDHEKSSLLGLWEERRILYEQCMDLQLFYRDTEQAETWMNKQEAFLANTDLGDSLDSVEHLIKKHEDFEKSLAAQEEKINALDEFATKLIQGQHYAADDVAERRQALLDRRRRLLDRARQRGNALKESYKRQTFDRDCDEMVSWITEKLSTARDDSYLDPTNIRGKLQKHINFEQELKANENRLDDIRSTGEQIIDSGHFAADHIGDRLRQVNNLWNELVDATNKKGAKLREAGNEQQFNRNIEDVEAWLSELEGQVASEDYGKDLVSVQNLQKKIGLIESDFNAHNDRVDGIKQLAQQFQEEEHFNAPVIVRKQENLQQRYNALRDPLEKRKRKLGESHQGNQLFRDIEDELAWIREKEQVAGSTNRGRDLIGVQNLIKKQQALIAEIANHDSQIESVSSAANDMIQQGHFLAPEIRDKLAQLRDNWRILKSKAEKRRTDLDDSLQAHQYLSDANEADAWMSEKEPIVGSTDYGKDEDSAEALLKKHRALLSDLEAFKGTIEDLRKQASQCKYQEQPMGQLGRDCVLALYDYQEKSPREVSMKKGDVLTLLNASNRDWWKVEVNDRQGFVPAAYVKRIEPGTAQQHAQQQVNSIGGKQSEIEDKYQRLMMLGETRKRKLEEACKGYQLLREANDLAEWIKSREAVAAQQEIGTDLEQVEVLQKKFDDFKGDLKANEVRLQEMNQIATALTSVGQTETAVRIRQQIEDLNARWRALEEQTEQREQQLGSAHEVQRFHRDVDETRDWIQEKDDALDSEDFGRDLRSVQALQRKHEGVERDLAALGDKIKSLDEKANRLRQSHPEAAEQIYDLQRELNEQWNRLTSKANNRKEKLLDSYDYQRFLSDYRDLMQWIASMNQLVSSQELANDVTGAEALLERHQEYRTEIDSRAATFQAFDQFGNQLLNSHHYAAGDIENRLQGVNEARKGLEDAWVARRNILDQCLELQLFYRDCEQADTWMSAREAFLAQEDPSGDNVESLIKKHEDFDKAINTQEEKIKGLKLFAESLIKNNHYDSPAVTRKRDQILDRWNGLKEALIQKRFKLGESQTLQQFSRDADEIENWMTEKFQIAQEENYRDPTNIQQKHQKQQAFEAELHANSDRIAAIIQAGNNLIENAKCGGGEAAVSARLKALNDQWDLLVKTTTEKSYRLKEANKQKSFMAAVKDLEFWLGEVEILLQSEDYGKDLASVENLLKKHSLLEADIVAHQDRVGEMNEQADSLLENDQFQGQQIAERRKLIADRYDGVKKMATDRRDKLSKALNVHQFFRDIDDEESWIKEKKLLVSSDDYGRDLPGVQNLRRKHRRIDTELASHEPQVSLVKLKGEELLRSAAEAGVGEDQIKKRMEDLEQSWGQIRDLTGNRHQRLDESEAFQAFLGDVEEEEAWMNEKQQILGSDNFGENMAGVQGLLKKHDTFQVDLELHKQRVADLINKGDTLIHNGNHHAPHIKQRCDQLRARLIEIESMAEGRLAKLRDNSAYLQFMWKCDVVESWIAEKEQQVRSEDFGRDLSSVQILLTKQEAFDAGLNAFEHEGIQRITELKDQLVSSEHQQSPAIEKRHTNVIQRWQNLLAHSEARRQKLLKMQQQFKQIEELYLAFAKKASTFNSWFENAEEDLTDPVRCNSLEEIRALRDAHGEFQRSLSSAEEDFRQLQDLDRRIKSFNVGPNPYTWFTMDALEDTWRNLQRIIKEREQELAREHQRQEENDKLRREFAKLANAFHAWLTNTRQEMMEAGGTLEEQLDAVERKAKEIKANKGQLRQIEEKGAMLERNLILDNRYTEHSTVGIAQAWDQLDQLAMRMQHNLEQQIQARNQSGVSEEALREFSMMFKHFDKEKTGRLDHQQFKSCLRALGYDLPMVDEGQPEPEFQRILDIVDPNRDGYVTLQEYMAFMISKETENIQSSEEIEMAFRALSKEFRPYVTAEELYANLTPEQAEFCIRRMKPYMDAISGRSIQGGLDYEQFVHALFQS

Target ORF information:

RefSeq Version	NM_001270070.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans SPeCtrin (spc-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001270070.2

ORF Insert Sequence:

ATGGCTGATT CGAATGACAC TCATGCTCCA CCAGAGCCGG TGCTAGAAGT ACCGCCACCA 
CAGGAGATTC GTATTCTGGA GACTGCTGAT GACATTCACC AACGTCGTCA GGAAGTATTA 
GGTCACTATT CAATTTTCAA GGATCACTCG CAGACAAAAC GAGATCGTCT CGAGGAAGCT 
CGTCAGTTTC AATATTTCAA ACGAGATGCT GATGAGTTGA ACGTTTGGAT TCTTGAAAAG 
CTCCAGACAG CTCAGGAAGA AAACTTCAGA GATCCGAGCA ATCTCCAGGC CAAGATCCAG 
AAGCATGAAG CGTTCGAAGC TGAGGTTCAA GCTCACGCTA AGACCATCGC CAACCTCGAC 
AAGACCGGAA ACGCTATGAT CCAACACAAT CATTTCGCTA GCGAACACAT CAAGAAACGC 
TTGGAAGAGT TGCACGCCCT CTGGGATAAG CTCTTCTTCA AACTGAAGGA TAAGGGAATT 
AAGCTACAAC AAGCACTTAA ACTTTTGCAT TTTATTCGTC AATGCGATGA AATTCTCTAC 
TGGATTCGAG AGAAGGAAAC CTATGTCACC GCAGAAGACA TGGGAATGGA TCTCGAACAC 
GTTGAGATAC TTCAGCGTAA ATTTGACGAC TTTTTGAAAG AACTCGGTAA CCATCAGTAC 
CGAATCAATG AGATCAATCA AGCTGCTGAC AAACTTGTCG AGGAGGGACA CTCTGAGCAC 
GATCAGATCT ACAAGAAGCG CGATGACGTT AATGATGCTT GGCATCGTTT GAACACTTTG 
GCGGCCACTC GTAAGGAAGG GCTCTTTGGA GCTCATCAAG TTCAACGCTT CAATCGCGAT 
GCAGACGAGA CCCTTGCATG GATTGGAGAG AAGGATCTTG CTTTGTCAAG CGATGATTAC 
GGAAGAGACC TCAATAATGT TCAAGCTCTT CAACGCAAGC ATGAAGGAAC TGAACGTGAT 
CTTGCTGCTC TTGAAGGAAA GATGCTCCAA TTGGAGAAAG AAGCTCATAA GTTGGCAGAA 
ACTCATCCAG ATCGTGCTGA AGCGATTCAT CAAAAGAACG ATGACACCAA AGCCGCCTGG 
AATGCACTCA AGGAGAAGGC TCAACACCGC AAAGATGGAC TTGAGAGAAG CTTCCAGCTT 
CATCGTTTCT TGGCCGACTA CCGTGACTTG ATTAGCTGGA TTGGAGACAT GAAAGCTGTC 
ATCGGAGCTG ATGAGCTTGC AAAAGACGTG GCTGGCGCTG AAGCTCTTCT TGAAAGTCAT 
CAAGAACACA AGGGAGAAAT TGATGCTCGT GCTGACTCGT TCAACCAAAC TGCATCCGCT 
GGACAAAAGC TCGTTGAGAT GGGAATTCCA GAGAGTCCAG AAGTTCAGGA GAAGCTCGAG 
AAACTTGACC ACGAGAAGTC TTCTCTTCTT GGATTGTGGG AGGAACGTCG TATTCTCTAC 
GAGCAATGTA TGGATCTCCA ACTCTTCTAT CGTGACACTG AACAAGCCGA AACCTGGATG 
AACAAGCAGG AAGCGTTCCT TGCCAACACT GATTTGGGAG ATTCACTAGA TTCTGTCGAG 
CACCTCATCA AGAAACACGA GGACTTTGAG AAATCGCTTG CTGCTCAAGA GGAGAAGATC 
AATGCACTTG ACGAGTTTGC TACCAAGCTC ATTCAAGGAC AGCATTACGC TGCTGATGAT 
GTTGCCGAGA GACGCCAAGC TCTTCTTGAC CGTCGTCGTC GTCTTCTAGA CAGAGCTCGT 
CAACGTGGAA ATGCTCTCAA GGAAAGCTAC AAGCGCCAGA CATTTGACCG TGACTGTGAT 
GAGATGGTTA GCTGGATCAC TGAAAAACTG TCCACCGCTC GTGACGATTC TTACTTGGAT 
CCTACCAACA TTCGTGGAAA ACTCCAAAAG CACATCAACT TTGAGCAAGA GCTTAAGGCT 
AACGAGAACC GTTTGGACGA TATTCGTTCT ACCGGAGAGC AAATCATCGA TAGTGGACAC 
TTTGCTGCTG ATCATATCGG AGACCGCCTT CGTCAAGTCA ACAACTTGTG GAACGAATTG 
GTCGATGCTA CAAACAAGAA GGGAGCCAAA CTTCGTGAAG CTGGAAACGA ACAGCAATTC 
AATCGCAACA TTGAAGATGT TGAAGCATGG TTGTCGGAGC TTGAAGGACA AGTTGCTTCC 
GAGGATTATG GAAAAGATCT TGTATCTGTT CAAAACCTTC AGAAGAAGAT TGGACTTATT 
GAGTCTGATT TTAACGCTCA CAACGATCGT GTTGATGGAA TCAAGCAACT TGCTCAACAG 
TTCCAAGAGG AGGAGCATTT CAATGCTCCA GTCATTGTTC GCAAGCAAGA GAATCTTCAG 
CAGCGCTACA ACGCTCTCCG TGATCCACTT GAGAAGAGAA AAAGAAAACT CGGAGAGTCC 
CACCAAGGAA ATCAACTGTT CCGCGATATT GAAGATGAGC TGGCTTGGAT CCGTGAGAAG 
GAACAAGTTG CTGGATCAAC CAATCGAGGA CGTGATTTGA TTGGAGTTCA AAATCTCATC 
AAGAAGCAGC AGGCTCTGAT CGCTGAGATT GCCAACCACG ATAGCCAAAT CGAGAGTGTC 
AGCAGTGCCG CCAATGACAT GATTCAACAA GGACACTTCT TGGCTCCAGA GATACGTGAT 
AAACTTGCTC AACTTCGTGA CAACTGGAGA ATTCTGAAGA GCAAGGCTGA GAAGAGACGC 
ACAGATCTTG ATGACTCACT CCAAGCCCAC CAATACCTTT CTGATGCCAA TGAGGCCGAT 
GCTTGGATGA GCGAGAAAGA ACCAATTGTT GGATCCACCG ACTATGGAAA GGACGAAGAT 
TCTGCCGAGG CTCTTCTCAA GAAGCATCGT GCTCTTCTTT CCGACTTGGA AGCTTTCAAG 
GGAACTATCG AGGATCTTCG CAAGCAAGCT TCTCAATGCA AGTACCAAGA GCAACCGATG 
GGACAACTCG GACGTGACTG TGTGTTGGCT CTTTACGATT ACCAAGAGAA ATCTCCTCGT 
GAGGTTTCCA TGAAGAAGGG AGATGTTCTC ACTCTTCTCA ATGCTAGCAA CAGAGACTGG 
TGGAAAGTTG AGGTCAATGA TCGTCAAGGA TTTGTTCCAG CTGCCTACGT TAAGAGAATC 
GAACCAGGAA CTGCTCAACA GCATGCTCAA CAACAGGTCA ACTCCATTGG AGGAAAGCAG 
TCGGAGATTG AGGACAAGTA CCAACGTCTC ATGATGCTCG GAGAGACTAG AAAGAGAAAG 
CTCGAGGAAG CTTGCAAAGG ATACCAACTT CTCCGTGAAG CCAACGACTT GGCTGAATGG 
ATCAAGTCTA GAGAGGCTGT TGCTGCTCAA CAAGAAATTG GAACTGACCT CGAGCAGGTT 
GAGGTTCTTC AAAAGAAGTT TGACGACTTT AAAGGAGACC TCAAGGCTAA CGAGGTTCGT 
CTCCAGGAGA TGAACCAAAT TGCCACCGCT CTCACATCGG TTGGACAAAC TGAAACTGCT 
GTTCGTATTC GTCAGCAAAT TGAGGATCTC AACGCCAGAT GGCGCGCATT GGAGGAGCAG 
ACTGAGCAAC GCGAACAACA ACTCGGATCT GCCCACGAGG TGCAACGCTT CCACCGTGAT 
GTCGACGAGA CTCGTGATTG GATCCAGGAG AAGGATGATG CCCTTGACAG TGAAGATTTT 
GGACGTGACC TCCGTTCCGT TCAGGCTCTT CAACGCAAGC ATGAAGGAGT TGAGCGTGAT 
TTGGCTGCTC TTGGAGACAA GATTAAATCT CTTGATGAGA AGGCCAACCG TCTTCGTCAA 
TCTCACCCAG AAGCTGCCGA GCAGATTTAC GATTTGCAAC GTGAGCTCAA TGAGCAATGG 
AACCGCCTCA CGTCTAAGGC CAACAACCGA AAAGAAAAGC TTCTTGATTC GTACGACTAC 
CAACGCTTTT TGTCTGACTA CCGTGATTTG ATGCAATGGA TTGCTTCCAT GAACCAACTT 
GTCAGCAGTC AAGAACTTGC CAACGATGTT ACTGGAGCTG AGGCTCTTCT TGAGCGCCAC 
CAGGAGTACC GCACCGAAAT TGACTCCCGT GCTGCCACCT TCCAAGCTTT TGATCAATTT 
GGAAACCAAC TTCTTAACAG CCACCACTAC GCTGCAGGAG ACATTGAAAA CCGTCTTCAA 
GGTGTCAACG AGGCTCGTAA AGGACTTGAA GACGCATGGG TTGCTCGTCG TAACATTCTC 
GATCAATGCC TCGAACTTCA ACTCTTCTAC CGTGATTGTG AGCAAGCCGA CACTTGGATG 
TCAGCTCGTG AAGCTTTCTT GGCTCAAGAG GATCCATCTG GAGACAATGT TGAGTCTTTG 
ATCAAGAAAC ACGAGGACTT TGACAAAGCC ATCAACACTC AAGAGGAGAA AATCAAGGGA 
CTCAAGCTCT TTGCTGAATC TCTCATCAAG AACAATCATT ACGATTCGCC AGCAGTCACT 
CGCAAGAGAG ACCAGATTCT CGATAGATGG AATGGTCTTA AGGAGGCTCT CATTCAGAAG 
AGATTCAAGC TTGGAGAGTC ACAAACTCTT CAACAGTTTA GTCGTGATGC GGATGAGATT 
GAGAACTGGA TGACTGAGAA GTTCCAGATT GCTCAAGAGG AGAACTACCG CGATCCAACC 
AACATTCAGC AGAAACATCA GAAGCAACAA GCCTTCGAAG CCGAGCTTCA TGCCAATTCG 
GACAGAATCG CTGCTATCAT TCAAGCTGGA AACAACCTCA TTGAGAACGC CAAGTGCGGA 
GGAGGTGAAG CTGCTGTCAG CGCTCGACTG AAGGCTCTCA ACGACCAATG GGACCTTCTT 
GTCAAGACCA CCACCGAGAA GAGTTACCGT TTGAAAGAGG CTAACAAGCA GAAGAGCTTC 
ATGGCTGCTG TCAAGGATCT TGAGTTCTGG CTGGGAGAGG TTGAAATTCT CCTCCAATCT 
GAAGACTATG GAAAAGATTT GGCTTCCGTT GAAAACCTGC TCAAAAAACA TTCTCTTCTT 
GAGGCTGACA TTGTTGCCCA CCAGGATCGT GTTGGAGAGA TGAACGAGCA AGCTGATTCT 
CTTCTCGAGA ACGATCAATT CCAAGGACAA CAAATCGCTG AGAGAAGAAA GTTGATTGCT 
GATCGTTATG ATGGTGTCAA GAAGATGGCT ACCGATCGTC GTGATAAATT GAGCAAGGCC 
CTCAATGTTC ACCAGTTCTT CCGTGATATT GATGATGAGG AGTCTTGGAT TAAGGAGAAG 
AAGCTTCTCG TTTCTTCTGA TGACTATGGA CGTGATCTTC CAGGAGTTCA AAACCTTCGC 
CGCAAGCATC GTCGTATTGA TACCGAGCTC GCTTCCCATG AGCCTCAAGT TTCTCTTGTC 
AAGCTGAAGG GAGAAGAGCT GTTGAGATCT GCAGCCGAAG CGGGAGTTGG AGAGGATCAA 
ATTAAGAAGC GTATGGAGGA TCTTGAGCAA AGCTGGGGAC AAATCCGTGA TCTTACTGGA 
AACCGTCATC AACGCCTCGA CGAGTCTGAA GCCTTCCAAG CTTTCTTGGG TGATGTTGAA 
GAGGAAGAGG CATGGATGAA TGAGAAACAA CAAATCCTGG GATCCGACAA CTTTGGAGAA 
AACATGGCTG GAGTGCAAGG ACTTCTCAAG AAGCACGATA CGTTCCAAGT CGATCTTGAG 
CTTCACAAGC AACGTGTTGC TGATTTGATC AACAAGGGAG ACACTCTTAT TCACAATGGA 
AACCATCATG CTCCTCATAT CAAGCAGAGA TGCGACCAAC TCCGTGCTCG TCTTATTGAG 
ATTGAGAGCA TGGCCGAAGG ACGTCTTGCC AAGCTTCGTG ATAACTCTGC CTACCTTCAA 
TTCATGTGGA AGTGTGATGT TGTGGAATCG TGGATTGCTG AGAAGGAGCA ACAAGTTCGT 
TCCGAAGACT TTGGAAGAGA TCTCTCATCT GTTCAAATCC TCCTCACCAA GCAAGAAGCT 
TTCGACGCTG GACTCAACGC TTTCGAACAT GAAGGAATTC AAAGAATCAC CGAGCTCAAA 
GACCAACTTG TTTCGAGTGA GCACCAACAA TCTCCAGCCA TTGAGAAACG TCATACGAAC 
GTCATCCAAC GTTGGCAGAA CCTTCTTGCC CACTCCGAGG CTCGTCGTCA GAAGTTGTTG 
AAAATGCAAC AGCAATTCAA GCAAATCGAG GAGCTTTACC TTGCCTTTGC CAAGAAGGCT 
TCCACTTTCA ACTCATGGTT CGAGAACGCT GAGGAGGACT TGACTGACCC TGTCCGATGC 
AACTCTTTGG AAGAGATCCG TGCCTTGCGC GACGCCCATG GTGAGTTCCA GCGCTCATTG 
TCCTCCGCTG AAGAGGACTT CCGCCAACTC CAAGACCTTG ATCGTCGGAT CAAGTCGTTC 
AATGTGGGAC CCAACCCTTA CACGTGGTTC ACTATGGATG CCCTCGAAGA CACGTGGCGC 
AACCTGCAGA GAATCATCAA GGAGCGCGAG CAGGAGCTTG CCAGAGAGCA CCAAAGACAG 
GAGGAGAACG ACAAATTGCG ACGCGAGTTT GCCAAGTTGG CCAATGCTTT CCATGCTTGG 
TTGACAAATA CCAGACAGGA GATGATGGAA GCTGGAGGTA CATTGGAAGA ACAACTCGAT 
GCTGTTGAGA GGAAAGCAAA GGAAATCAAG GCGAACAAAG GTCAGCTGCG GCAGATTGAA 
GAGAAGGGTG CTATGTTGGA GAGAAACCTT ATCCTAGACA ATAGGTATAC TGAACATTCG 
ACAGTAGGAA TTGCGCAAGC TTGGGATCAA TTGGATCAGC TAGCCATGCG AATGCAACAC 
AATTTGGAAC AACAGATTCA AGCCAGAAAT CAATCTGGAG TATCGGAAGA AGCCCTTCGT 
GAGTTCTCAA TGATGTTCAA GCACTTCGAC AAGGAGAAAA CTGGAAGACT CGACCACCAG 
CAGTTCAAGA GTTGTCTGCG AGCTCTTGGA TACGATCTGC CGATGGTGGA CGAAGGCCAA 
CCAGAACCCG AGTTCCAGAG AATTTTGGAT ATTGTCGACC CGAACAGAGA TGGTTACGTA 
ACTCTGCAAG AGTACATGGC GTTCATGATC AGCAAAGAAA CCGAGAACAT CCAGTCTTCG 
GAAGAAATCG AGATGGCATT CAGAGCTCTT TCGAAGGAAT TCCGACCATA TGTCACGGCA 
GAGGAGCTCT ACGCGAACCT GACACCGGAG CAAGCCGAAT TCTGCATACG AAGAATGAAG 
CCATACATGG ACGCCATTTC AGGTCGTTCC ATCCAAGGTG GTCTCGACTA CGAGCAGTTT 
GTGCATGCCT TGTTCCAGAG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spc-1 ( NM_001270070.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001256999.1 Caenorhabditis elegans SPeCtrin (spc-1), partial mRNA.

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CloneID	OCa349360
Clone ID Related Accession (Same CDS sequence)	NM_001270070.2 , NM_001270070.1
Accession Version	NM_001270070.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7284bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	SPeCtrin
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). COMPLETENESS: incomplete on both ends.

ATGGCTGATT CGAATGACAC TCATGCTCCA CCAGAGCCGG TGCTAGAAGT ACCGCCACCA 
CAGGAGATTC GTATTCTGGA GACTGCTGAT GACATTCACC AACGTCGTCA GGAAGTATTA 
GGTCACTATT CAATTTTCAA GGATCACTCG CAGACAAAAC GAGATCGTCT CGAGGAAGCT 
CGTCAGTTTC AATATTTCAA ACGAGATGCT GATGAGTTGA ACGTTTGGAT TCTTGAAAAG 
CTCCAGACAG CTCAGGAAGA AAACTTCAGA GATCCGAGCA ATCTCCAGGC CAAGATCCAG 
AAGCATGAAG CGTTCGAAGC TGAGGTTCAA GCTCACGCTA AGACCATCGC CAACCTCGAC 
AAGACCGGAA ACGCTATGAT CCAACACAAT CATTTCGCTA GCGAACACAT CAAGAAACGC 
TTGGAAGAGT TGCACGCCCT CTGGGATAAG CTCTTCTTCA AACTGAAGGA TAAGGGAATT 
AAGCTACAAC AAGCACTTAA ACTTTTGCAT TTTATTCGTC AATGCGATGA AATTCTCTAC 
TGGATTCGAG AGAAGGAAAC CTATGTCACC GCAGAAGACA TGGGAATGGA TCTCGAACAC 
GTTGAGATAC TTCAGCGTAA ATTTGACGAC TTTTTGAAAG AACTCGGTAA CCATCAGTAC 
CGAATCAATG AGATCAATCA AGCTGCTGAC AAACTTGTCG AGGAGGGACA CTCTGAGCAC 
GATCAGATCT ACAAGAAGCG CGATGACGTT AATGATGCTT GGCATCGTTT GAACACTTTG 
GCGGCCACTC GTAAGGAAGG GCTCTTTGGA GCTCATCAAG TTCAACGCTT CAATCGCGAT 
GCAGACGAGA CCCTTGCATG GATTGGAGAG AAGGATCTTG CTTTGTCAAG CGATGATTAC 
GGAAGAGACC TCAATAATGT TCAAGCTCTT CAACGCAAGC ATGAAGGAAC TGAACGTGAT 
CTTGCTGCTC TTGAAGGAAA GATGCTCCAA TTGGAGAAAG AAGCTCATAA GTTGGCAGAA 
ACTCATCCAG ATCGTGCTGA AGCGATTCAT CAAAAGAACG ATGACACCAA AGCCGCCTGG 
AATGCACTCA AGGAGAAGGC TCAACACCGC AAAGATGGAC TTGAGAGAAG CTTCCAGCTT 
CATCGTTTCT TGGCCGACTA CCGTGACTTG ATTAGCTGGA TTGGAGACAT GAAAGCTGTC 
ATCGGAGCTG ATGAGCTTGC AAAAGACGTG GCTGGCGCTG AAGCTCTTCT TGAAAGTCAT 
CAAGAACACA AGGGAGAAAT TGATGCTCGT GCTGACTCGT TCAACCAAAC TGCATCCGCT 
GGACAAAAGC TCGTTGAGAT GGGAATTCCA GAGAGTCCAG AAGTTCAGGA GAAGCTCGAG 
AAACTTGACC ACGAGAAGTC TTCTCTTCTT GGATTGTGGG AGGAACGTCG TATTCTCTAC 
GAGCAATGTA TGGATCTCCA ACTCTTCTAT CGTGACACTG AACAAGCCGA AACCTGGATG 
AACAAGCAGG AAGCGTTCCT TGCCAACACT GATTTGGGAG ATTCACTAGA TTCTGTCGAG 
CACCTCATCA AGAAACACGA GGACTTTGAG AAATCGCTTG CTGCTCAAGA GGAGAAGATC 
AATGCACTTG ACGAGTTTGC TACCAAGCTC ATTCAAGGAC AGCATTACGC TGCTGATGAT 
GTTGCCGAGA GACGCCAAGC TCTTCTTGAC CGTCGTCGTC GTCTTCTAGA CAGAGCTCGT 
CAACGTGGAA ATGCTCTCAA GGAAAGCTAC AAGCGCCAGA CATTTGACCG TGACTGTGAT 
GAGATGGTTA GCTGGATCAC TGAAAAACTG TCCACCGCTC GTGACGATTC TTACTTGGAT 
CCTACCAACA TTCGTGGAAA ACTCCAAAAG CACATCAACT TTGAGCAAGA GCTTAAGGCT 
AACGAGAACC GTTTGGACGA TATTCGTTCT ACCGGAGAGC AAATCATCGA TAGTGGACAC 
TTTGCTGCTG ATCATATCGG AGACCGCCTT CGTCAAGTCA ACAACTTGTG GAACGAATTG 
GTCGATGCTA CAAACAAGAA GGGAGCCAAA CTTCGTGAAG CTGGAAACGA ACAGCAATTC 
AATCGCAACA TTGAAGATGT TGAAGCATGG TTGTCGGAGC TTGAAGGACA AGTTGCTTCC 
GAGGATTATG GAAAAGATCT TGTATCTGTT CAAAACCTTC AGAAGAAGAT TGGACTTATT 
GAGTCTGATT TTAACGCTCA CAACGATCGT GTTGATGGAA TCAAGCAACT TGCTCAACAG 
TTCCAAGAGG AGGAGCATTT CAATGCTCCA GTCATTGTTC GCAAGCAAGA GAATCTTCAG 
CAGCGCTACA ACGCTCTCCG TGATCCACTT GAGAAGAGAA AAAGAAAACT CGGAGAGTCC 
CACCAAGGAA ATCAACTGTT CCGCGATATT GAAGATGAGC TGGCTTGGAT CCGTGAGAAG 
GAACAAGTTG CTGGATCAAC CAATCGAGGA CGTGATTTGA TTGGAGTTCA AAATCTCATC 
AAGAAGCAGC AGGCTCTGAT CGCTGAGATT GCCAACCACG ATAGCCAAAT CGAGAGTGTC 
AGCAGTGCCG CCAATGACAT GATTCAACAA GGACACTTCT TGGCTCCAGA GATACGTGAT 
AAACTTGCTC AACTTCGTGA CAACTGGAGA ATTCTGAAGA GCAAGGCTGA GAAGAGACGC 
ACAGATCTTG ATGACTCACT CCAAGCCCAC CAATACCTTT CTGATGCCAA TGAGGCCGAT 
GCTTGGATGA GCGAGAAAGA ACCAATTGTT GGATCCACCG ACTATGGAAA GGACGAAGAT 
TCTGCCGAGG CTCTTCTCAA GAAGCATCGT GCTCTTCTTT CCGACTTGGA AGCTTTCAAG 
GGAACTATCG AGGATCTTCG CAAGCAAGCT TCTCAATGCA AGTACCAAGA GCAACCGATG 
GGACAACTCG GACGTGACTG TGTGTTGGCT CTTTACGATT ACCAAGAGAA ATCTCCTCGT 
GAGGTTTCCA TGAAGAAGGG AGATGTTCTC ACTCTTCTCA ATGCTAGCAA CAGAGACTGG 
TGGAAAGTTG AGGTCAATGA TCGTCAAGGA TTTGTTCCAG CTGCCTACGT TAAGAGAATC 
GAACCAGGAA CTGCTCAACA GCATGCTCAA CAACAGGTCA ACTCCATTGG AGGAAAGCAG 
TCGGAGATTG AGGACAAGTA CCAACGTCTC ATGATGCTCG GAGAGACTAG AAAGAGAAAG 
CTCGAGGAAG CTTGCAAAGG ATACCAACTT CTCCGTGAAG CCAACGACTT GGCTGAATGG 
ATCAAGTCTA GAGAGGCTGT TGCTGCTCAA CAAGAAATTG GAACTGACCT CGAGCAGGTT 
GAGGTTCTTC AAAAGAAGTT TGACGACTTT AAAGGAGACC TCAAGGCTAA CGAGGTTCGT 
CTCCAGGAGA TGAACCAAAT TGCCACCGCT CTCACATCGG TTGGACAAAC TGAAACTGCT 
GTTCGTATTC GTCAGCAAAT TGAGGATCTC AACGCCAGAT GGCGCGCATT GGAGGAGCAG 
ACTGAGCAAC GCGAACAACA ACTCGGATCT GCCCACGAGG TGCAACGCTT CCACCGTGAT 
GTCGACGAGA CTCGTGATTG GATCCAGGAG AAGGATGATG CCCTTGACAG TGAAGATTTT 
GGACGTGACC TCCGTTCCGT TCAGGCTCTT CAACGCAAGC ATGAAGGAGT TGAGCGTGAT 
TTGGCTGCTC TTGGAGACAA GATTAAATCT CTTGATGAGA AGGCCAACCG TCTTCGTCAA 
TCTCACCCAG AAGCTGCCGA GCAGATTTAC GATTTGCAAC GTGAGCTCAA TGAGCAATGG 
AACCGCCTCA CGTCTAAGGC CAACAACCGA AAAGAAAAGC TTCTTGATTC GTACGACTAC 
CAACGCTTTT TGTCTGACTA CCGTGATTTG ATGCAATGGA TTGCTTCCAT GAACCAACTT 
GTCAGCAGTC AAGAACTTGC CAACGATGTT ACTGGAGCTG AGGCTCTTCT TGAGCGCCAC 
CAGGAGTACC GCACCGAAAT TGACTCCCGT GCTGCCACCT TCCAAGCTTT TGATCAATTT 
GGAAACCAAC TTCTTAACAG CCACCACTAC GCTGCAGGAG ACATTGAAAA CCGTCTTCAA 
GGTGTCAACG AGGCTCGTAA AGGACTTGAA GACGCATGGG TTGCTCGTCG TAACATTCTC 
GATCAATGCC TCGAACTTCA ACTCTTCTAC CGTGATTGTG AGCAAGCCGA CACTTGGATG 
TCAGCTCGTG AAGCTTTCTT GGCTCAAGAG GATCCATCTG GAGACAATGT TGAGTCTTTG 
ATCAAGAAAC ACGAGGACTT TGACAAAGCC ATCAACACTC AAGAGGAGAA AATCAAGGGA 
CTCAAGCTCT TTGCTGAATC TCTCATCAAG AACAATCATT ACGATTCGCC AGCAGTCACT 
CGCAAGAGAG ACCAGATTCT CGATAGATGG AATGGTCTTA AGGAGGCTCT CATTCAGAAG 
AGATTCAAGC TTGGAGAGTC ACAAACTCTT CAACAGTTTA GTCGTGATGC GGATGAGATT 
GAGAACTGGA TGACTGAGAA GTTCCAGATT GCTCAAGAGG AGAACTACCG CGATCCAACC 
AACATTCAGC AGAAACATCA GAAGCAACAA GCCTTCGAAG CCGAGCTTCA TGCCAATTCG 
GACAGAATCG CTGCTATCAT TCAAGCTGGA AACAACCTCA TTGAGAACGC CAAGTGCGGA 
GGAGGTGAAG CTGCTGTCAG CGCTCGACTG AAGGCTCTCA ACGACCAATG GGACCTTCTT 
GTCAAGACCA CCACCGAGAA GAGTTACCGT TTGAAAGAGG CTAACAAGCA GAAGAGCTTC 
ATGGCTGCTG TCAAGGATCT TGAGTTCTGG CTGGGAGAGG TTGAAATTCT CCTCCAATCT 
GAAGACTATG GAAAAGATTT GGCTTCCGTT GAAAACCTGC TCAAAAAACA TTCTCTTCTT 
GAGGCTGACA TTGTTGCCCA CCAGGATCGT GTTGGAGAGA TGAACGAGCA AGCTGATTCT 
CTTCTCGAGA ACGATCAATT CCAAGGACAA CAAATCGCTG AGAGAAGAAA GTTGATTGCT 
GATCGTTATG ATGGTGTCAA GAAGATGGCT ACCGATCGTC GTGATAAATT GAGCAAGGCC 
CTCAATGTTC ACCAGTTCTT CCGTGATATT GATGATGAGG AGTCTTGGAT TAAGGAGAAG 
AAGCTTCTCG TTTCTTCTGA TGACTATGGA CGTGATCTTC CAGGAGTTCA AAACCTTCGC 
CGCAAGCATC GTCGTATTGA TACCGAGCTC GCTTCCCATG AGCCTCAAGT TTCTCTTGTC 
AAGCTGAAGG GAGAAGAGCT GTTGAGATCT GCAGCCGAAG CGGGAGTTGG AGAGGATCAA 
ATTAAGAAGC GTATGGAGGA TCTTGAGCAA AGCTGGGGAC AAATCCGTGA TCTTACTGGA 
AACCGTCATC AACGCCTCGA CGAGTCTGAA GCCTTCCAAG CTTTCTTGGG TGATGTTGAA 
GAGGAAGAGG CATGGATGAA TGAGAAACAA CAAATCCTGG GATCCGACAA CTTTGGAGAA 
AACATGGCTG GAGTGCAAGG ACTTCTCAAG AAGCACGATA CGTTCCAAGT CGATCTTGAG 
CTTCACAAGC AACGTGTTGC TGATTTGATC AACAAGGGAG ACACTCTTAT TCACAATGGA 
AACCATCATG CTCCTCATAT CAAGCAGAGA TGCGACCAAC TCCGTGCTCG TCTTATTGAG 
ATTGAGAGCA TGGCCGAAGG ACGTCTTGCC AAGCTTCGTG ATAACTCTGC CTACCTTCAA 
TTCATGTGGA AGTGTGATGT TGTGGAATCG TGGATTGCTG AGAAGGAGCA ACAAGTTCGT 
TCCGAAGACT TTGGAAGAGA TCTCTCATCT GTTCAAATCC TCCTCACCAA GCAAGAAGCT 
TTCGACGCTG GACTCAACGC TTTCGAACAT GAAGGAATTC AAAGAATCAC CGAGCTCAAA 
GACCAACTTG TTTCGAGTGA GCACCAACAA TCTCCAGCCA TTGAGAAACG TCATACGAAC 
GTCATCCAAC GTTGGCAGAA CCTTCTTGCC CACTCCGAGG CTCGTCGTCA GAAGTTGTTG 
AAAATGCAAC AGCAATTCAA GCAAATCGAG GAGCTTTACC TTGCCTTTGC CAAGAAGGCT 
TCCACTTTCA ACTCATGGTT CGAGAACGCT GAGGAGGACT TGACTGACCC TGTCCGATGC 
AACTCTTTGG AAGAGATCCG TGCCTTGCGC GACGCCCATG GTGAGTTCCA GCGCTCATTG 
TCCTCCGCTG AAGAGGACTT CCGCCAACTC CAAGACCTTG ATCGTCGGAT CAAGTCGTTC 
AATGTGGGAC CCAACCCTTA CACGTGGTTC ACTATGGATG CCCTCGAAGA CACGTGGCGC 
AACCTGCAGA GAATCATCAA GGAGCGCGAG CAGGAGCTTG CCAGAGAGCA CCAAAGACAG 
GAGGAGAACG ACAAATTGCG ACGCGAGTTT GCCAAGTTGG CCAATGCTTT CCATGCTTGG 
TTGACAAATA CCAGACAGGA GATGATGGAA GCTGGAGGTA CATTGGAAGA ACAACTCGAT 
GCTGTTGAGA GGAAAGCAAA GGAAATCAAG GCGAACAAAG GTCAGCTGCG GCAGATTGAA 
GAGAAGGGTG CTATGTTGGA GAGAAACCTT ATCCTAGACA ATAGGTATAC TGAACATTCG 
ACAGTAGGAA TTGCGCAAGC TTGGGATCAA TTGGATCAGC TAGCCATGCG AATGCAACAC 
AATTTGGAAC AACAGATTCA AGCCAGAAAT CAATCTGGAG TATCGGAAGA AGCCCTTCGT 
GAGTTCTCAA TGATGTTCAA GCACTTCGAC AAGGAGAAAA CTGGAAGACT CGACCACCAG 
CAGTTCAAGA GTTGTCTGCG AGCTCTTGGA TACGATCTGC CGATGGTGGA CGAAGGCCAA 
CCAGAACCCG AGTTCCAGAG AATTTTGGAT ATTGTCGACC CGAACAGAGA TGGTTACGTA 
ACTCTGCAAG AGTACATGGC GTTCATGATC AGCAAAGAAA CCGAGAACAT CCAGTCTTCG 
GAAGAAATCG AGATGGCATT CAGAGCTCTT TCGAAGGAAT TCCGACCATA TGTCACGGCA 
GAGGAGCTCT ACGCGAACCT GACACCGGAG CAAGCCGAAT TCTGCATACG AAGAATGAAG 
CCATACATGG ACGCCATTTC AGGTCGTTCC ATCCAAGGTG GTCTCGACTA CGAGCAGTTT 
GTGCATGCCT TGTTCCAGAG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001256999.1
CDS	1..7284
Translation	MADSNDTHAPPEPVLEVPPPQEIRILETADDIHQRRQEVLGHYSIFKDHSQTKRDRLEEARQFQYFKRDADELNVWILEKLQTAQEENFRDPSNLQAKIQKHEAFEAEVQAHAKTIANLDKTGNAMIQHNHFASEHIKKRLEELHALWDKLFFKLKDKGIKLQQALKLLHFIRQCDEILYWIREKETYVTAEDMGMDLEHVEILQRKFDDFLKELGNHQYRINEINQAADKLVEEGHSEHDQIYKKRDDVNDAWHRLNTLAATRKEGLFGAHQVQRFNRDADETLAWIGEKDLALSSDDYGRDLNNVQALQRKHEGTERDLAALEGKMLQLEKEAHKLAETHPDRAEAIHQKNDDTKAAWNALKEKAQHRKDGLERSFQLHRFLADYRDLISWIGDMKAVIGADELAKDVAGAEALLESHQEHKGEIDARADSFNQTASAGQKLVEMGIPESPEVQEKLEKLDHEKSSLLGLWEERRILYEQCMDLQLFYRDTEQAETWMNKQEAFLANTDLGDSLDSVEHLIKKHEDFEKSLAAQEEKINALDEFATKLIQGQHYAADDVAERRQALLDRRRRLLDRARQRGNALKESYKRQTFDRDCDEMVSWITEKLSTARDDSYLDPTNIRGKLQKHINFEQELKANENRLDDIRSTGEQIIDSGHFAADHIGDRLRQVNNLWNELVDATNKKGAKLREAGNEQQFNRNIEDVEAWLSELEGQVASEDYGKDLVSVQNLQKKIGLIESDFNAHNDRVDGIKQLAQQFQEEEHFNAPVIVRKQENLQQRYNALRDPLEKRKRKLGESHQGNQLFRDIEDELAWIREKEQVAGSTNRGRDLIGVQNLIKKQQALIAEIANHDSQIESVSSAANDMIQQGHFLAPEIRDKLAQLRDNWRILKSKAEKRRTDLDDSLQAHQYLSDANEADAWMSEKEPIVGSTDYGKDEDSAEALLKKHRALLSDLEAFKGTIEDLRKQASQCKYQEQPMGQLGRDCVLALYDYQEKSPREVSMKKGDVLTLLNASNRDWWKVEVNDRQGFVPAAYVKRIEPGTAQQHAQQQVNSIGGKQSEIEDKYQRLMMLGETRKRKLEEACKGYQLLREANDLAEWIKSREAVAAQQEIGTDLEQVEVLQKKFDDFKGDLKANEVRLQEMNQIATALTSVGQTETAVRIRQQIEDLNARWRALEEQTEQREQQLGSAHEVQRFHRDVDETRDWIQEKDDALDSEDFGRDLRSVQALQRKHEGVERDLAALGDKIKSLDEKANRLRQSHPEAAEQIYDLQRELNEQWNRLTSKANNRKEKLLDSYDYQRFLSDYRDLMQWIASMNQLVSSQELANDVTGAEALLERHQEYRTEIDSRAATFQAFDQFGNQLLNSHHYAAGDIENRLQGVNEARKGLEDAWVARRNILDQCLELQLFYRDCEQADTWMSAREAFLAQEDPSGDNVESLIKKHEDFDKAINTQEEKIKGLKLFAESLIKNNHYDSPAVTRKRDQILDRWNGLKEALIQKRFKLGESQTLQQFSRDADEIENWMTEKFQIAQEENYRDPTNIQQKHQKQQAFEAELHANSDRIAAIIQAGNNLIENAKCGGGEAAVSARLKALNDQWDLLVKTTTEKSYRLKEANKQKSFMAAVKDLEFWLGEVEILLQSEDYGKDLASVENLLKKHSLLEADIVAHQDRVGEMNEQADSLLENDQFQGQQIAERRKLIADRYDGVKKMATDRRDKLSKALNVHQFFRDIDDEESWIKEKKLLVSSDDYGRDLPGVQNLRRKHRRIDTELASHEPQVSLVKLKGEELLRSAAEAGVGEDQIKKRMEDLEQSWGQIRDLTGNRHQRLDESEAFQAFLGDVEEEEAWMNEKQQILGSDNFGENMAGVQGLLKKHDTFQVDLELHKQRVADLINKGDTLIHNGNHHAPHIKQRCDQLRARLIEIESMAEGRLAKLRDNSAYLQFMWKCDVVESWIAEKEQQVRSEDFGRDLSSVQILLTKQEAFDAGLNAFEHEGIQRITELKDQLVSSEHQQSPAIEKRHTNVIQRWQNLLAHSEARRQKLLKMQQQFKQIEELYLAFAKKASTFNSWFENAEEDLTDPVRCNSLEEIRALRDAHGEFQRSLSSAEEDFRQLQDLDRRIKSFNVGPNPYTWFTMDALEDTWRNLQRIIKEREQELAREHQRQEENDKLRREFAKLANAFHAWLTNTRQEMMEAGGTLEEQLDAVERKAKEIKANKGQLRQIEEKGAMLERNLILDNRYTEHSTVGIAQAWDQLDQLAMRMQHNLEQQIQARNQSGVSEEALREFSMMFKHFDKEKTGRLDHQQFKSCLRALGYDLPMVDEGQPEPEFQRILDIVDPNRDGYVTLQEYMAFMISKETENIQSSEEIEMAFRALSKEFRPYVTAEELYANLTPEQAEFCIRRMKPYMDAISGRSIQGGLDYEQFVHALFQS

Target ORF information:

RefSeq Version	NM_001270070.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans SPeCtrin (spc-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001270070.1

ORF Insert Sequence:

ATGGCTGATT CGAATGACAC TCATGCTCCA CCAGAGCCGG TGCTAGAAGT ACCGCCACCA 
CAGGAGATTC GTATTCTGGA GACTGCTGAT GACATTCACC AACGTCGTCA GGAAGTATTA 
GGTCACTATT CAATTTTCAA GGATCACTCG CAGACAAAAC GAGATCGTCT CGAGGAAGCT 
CGTCAGTTTC AATATTTCAA ACGAGATGCT GATGAGTTGA ACGTTTGGAT TCTTGAAAAG 
CTCCAGACAG CTCAGGAAGA AAACTTCAGA GATCCGAGCA ATCTCCAGGC CAAGATCCAG 
AAGCATGAAG CGTTCGAAGC TGAGGTTCAA GCTCACGCTA AGACCATCGC CAACCTCGAC 
AAGACCGGAA ACGCTATGAT CCAACACAAT CATTTCGCTA GCGAACACAT CAAGAAACGC 
TTGGAAGAGT TGCACGCCCT CTGGGATAAG CTCTTCTTCA AACTGAAGGA TAAGGGAATT 
AAGCTACAAC AAGCACTTAA ACTTTTGCAT TTTATTCGTC AATGCGATGA AATTCTCTAC 
TGGATTCGAG AGAAGGAAAC CTATGTCACC GCAGAAGACA TGGGAATGGA TCTCGAACAC 
GTTGAGATAC TTCAGCGTAA ATTTGACGAC TTTTTGAAAG AACTCGGTAA CCATCAGTAC 
CGAATCAATG AGATCAATCA AGCTGCTGAC AAACTTGTCG AGGAGGGACA CTCTGAGCAC 
GATCAGATCT ACAAGAAGCG CGATGACGTT AATGATGCTT GGCATCGTTT GAACACTTTG 
GCGGCCACTC GTAAGGAAGG GCTCTTTGGA GCTCATCAAG TTCAACGCTT CAATCGCGAT 
GCAGACGAGA CCCTTGCATG GATTGGAGAG AAGGATCTTG CTTTGTCAAG CGATGATTAC 
GGAAGAGACC TCAATAATGT TCAAGCTCTT CAACGCAAGC ATGAAGGAAC TGAACGTGAT 
CTTGCTGCTC TTGAAGGAAA GATGCTCCAA TTGGAGAAAG AAGCTCATAA GTTGGCAGAA 
ACTCATCCAG ATCGTGCTGA AGCGATTCAT CAAAAGAACG ATGACACCAA AGCCGCCTGG 
AATGCACTCA AGGAGAAGGC TCAACACCGC AAAGATGGAC TTGAGAGAAG CTTCCAGCTT 
CATCGTTTCT TGGCCGACTA CCGTGACTTG ATTAGCTGGA TTGGAGACAT GAAAGCTGTC 
ATCGGAGCTG ATGAGCTTGC AAAAGACGTG GCTGGCGCTG AAGCTCTTCT TGAAAGTCAT 
CAAGAACACA AGGGAGAAAT TGATGCTCGT GCTGACTCGT TCAACCAAAC TGCATCCGCT 
GGACAAAAGC TCGTTGAGAT GGGAATTCCA GAGAGTCCAG AAGTTCAGGA GAAGCTCGAG 
AAACTTGACC ACGAGAAGTC TTCTCTTCTT GGATTGTGGG AGGAACGTCG TATTCTCTAC 
GAGCAATGTA TGGATCTCCA ACTCTTCTAT CGTGACACTG AACAAGCCGA AACCTGGATG 
AACAAGCAGG AAGCGTTCCT TGCCAACACT GATTTGGGAG ATTCACTAGA TTCTGTCGAG 
CACCTCATCA AGAAACACGA GGACTTTGAG AAATCGCTTG CTGCTCAAGA GGAGAAGATC 
AATGCACTTG ACGAGTTTGC TACCAAGCTC ATTCAAGGAC AGCATTACGC TGCTGATGAT 
GTTGCCGAGA GACGCCAAGC TCTTCTTGAC CGTCGTCGTC GTCTTCTAGA CAGAGCTCGT 
CAACGTGGAA ATGCTCTCAA GGAAAGCTAC AAGCGCCAGA CATTTGACCG TGACTGTGAT 
GAGATGGTTA GCTGGATCAC TGAAAAACTG TCCACCGCTC GTGACGATTC TTACTTGGAT 
CCTACCAACA TTCGTGGAAA ACTCCAAAAG CACATCAACT TTGAGCAAGA GCTTAAGGCT 
AACGAGAACC GTTTGGACGA TATTCGTTCT ACCGGAGAGC AAATCATCGA TAGTGGACAC 
TTTGCTGCTG ATCATATCGG AGACCGCCTT CGTCAAGTCA ACAACTTGTG GAACGAATTG 
GTCGATGCTA CAAACAAGAA GGGAGCCAAA CTTCGTGAAG CTGGAAACGA ACAGCAATTC 
AATCGCAACA TTGAAGATGT TGAAGCATGG TTGTCGGAGC TTGAAGGACA AGTTGCTTCC 
GAGGATTATG GAAAAGATCT TGTATCTGTT CAAAACCTTC AGAAGAAGAT TGGACTTATT 
GAGTCTGATT TTAACGCTCA CAACGATCGT GTTGATGGAA TCAAGCAACT TGCTCAACAG 
TTCCAAGAGG AGGAGCATTT CAATGCTCCA GTCATTGTTC GCAAGCAAGA GAATCTTCAG 
CAGCGCTACA ACGCTCTCCG TGATCCACTT GAGAAGAGAA AAAGAAAACT CGGAGAGTCC 
CACCAAGGAA ATCAACTGTT CCGCGATATT GAAGATGAGC TGGCTTGGAT CCGTGAGAAG 
GAACAAGTTG CTGGATCAAC CAATCGAGGA CGTGATTTGA TTGGAGTTCA AAATCTCATC 
AAGAAGCAGC AGGCTCTGAT CGCTGAGATT GCCAACCACG ATAGCCAAAT CGAGAGTGTC 
AGCAGTGCCG CCAATGACAT GATTCAACAA GGACACTTCT TGGCTCCAGA GATACGTGAT 
AAACTTGCTC AACTTCGTGA CAACTGGAGA ATTCTGAAGA GCAAGGCTGA GAAGAGACGC 
ACAGATCTTG ATGACTCACT CCAAGCCCAC CAATACCTTT CTGATGCCAA TGAGGCCGAT 
GCTTGGATGA GCGAGAAAGA ACCAATTGTT GGATCCACCG ACTATGGAAA GGACGAAGAT 
TCTGCCGAGG CTCTTCTCAA GAAGCATCGT GCTCTTCTTT CCGACTTGGA AGCTTTCAAG 
GGAACTATCG AGGATCTTCG CAAGCAAGCT TCTCAATGCA AGTACCAAGA GCAACCGATG 
GGACAACTCG GACGTGACTG TGTGTTGGCT CTTTACGATT ACCAAGAGAA ATCTCCTCGT 
GAGGTTTCCA TGAAGAAGGG AGATGTTCTC ACTCTTCTCA ATGCTAGCAA CAGAGACTGG 
TGGAAAGTTG AGGTCAATGA TCGTCAAGGA TTTGTTCCAG CTGCCTACGT TAAGAGAATC 
GAACCAGGAA CTGCTCAACA GCATGCTCAA CAACAGGTCA ACTCCATTGG AGGAAAGCAG 
TCGGAGATTG AGGACAAGTA CCAACGTCTC ATGATGCTCG GAGAGACTAG AAAGAGAAAG 
CTCGAGGAAG CTTGCAAAGG ATACCAACTT CTCCGTGAAG CCAACGACTT GGCTGAATGG 
ATCAAGTCTA GAGAGGCTGT TGCTGCTCAA CAAGAAATTG GAACTGACCT CGAGCAGGTT 
GAGGTTCTTC AAAAGAAGTT TGACGACTTT AAAGGAGACC TCAAGGCTAA CGAGGTTCGT 
CTCCAGGAGA TGAACCAAAT TGCCACCGCT CTCACATCGG TTGGACAAAC TGAAACTGCT 
GTTCGTATTC GTCAGCAAAT TGAGGATCTC AACGCCAGAT GGCGCGCATT GGAGGAGCAG 
ACTGAGCAAC GCGAACAACA ACTCGGATCT GCCCACGAGG TGCAACGCTT CCACCGTGAT 
GTCGACGAGA CTCGTGATTG GATCCAGGAG AAGGATGATG CCCTTGACAG TGAAGATTTT 
GGACGTGACC TCCGTTCCGT TCAGGCTCTT CAACGCAAGC ATGAAGGAGT TGAGCGTGAT 
TTGGCTGCTC TTGGAGACAA GATTAAATCT CTTGATGAGA AGGCCAACCG TCTTCGTCAA 
TCTCACCCAG AAGCTGCCGA GCAGATTTAC GATTTGCAAC GTGAGCTCAA TGAGCAATGG 
AACCGCCTCA CGTCTAAGGC CAACAACCGA AAAGAAAAGC TTCTTGATTC GTACGACTAC 
CAACGCTTTT TGTCTGACTA CCGTGATTTG ATGCAATGGA TTGCTTCCAT GAACCAACTT 
GTCAGCAGTC AAGAACTTGC CAACGATGTT ACTGGAGCTG AGGCTCTTCT TGAGCGCCAC 
CAGGAGTACC GCACCGAAAT TGACTCCCGT GCTGCCACCT TCCAAGCTTT TGATCAATTT 
GGAAACCAAC TTCTTAACAG CCACCACTAC GCTGCAGGAG ACATTGAAAA CCGTCTTCAA 
GGTGTCAACG AGGCTCGTAA AGGACTTGAA GACGCATGGG TTGCTCGTCG TAACATTCTC 
GATCAATGCC TCGAACTTCA ACTCTTCTAC CGTGATTGTG AGCAAGCCGA CACTTGGATG 
TCAGCTCGTG AAGCTTTCTT GGCTCAAGAG GATCCATCTG GAGACAATGT TGAGTCTTTG 
ATCAAGAAAC ACGAGGACTT TGACAAAGCC ATCAACACTC AAGAGGAGAA AATCAAGGGA 
CTCAAGCTCT TTGCTGAATC TCTCATCAAG AACAATCATT ACGATTCGCC AGCAGTCACT 
CGCAAGAGAG ACCAGATTCT CGATAGATGG AATGGTCTTA AGGAGGCTCT CATTCAGAAG 
AGATTCAAGC TTGGAGAGTC ACAAACTCTT CAACAGTTTA GTCGTGATGC GGATGAGATT 
GAGAACTGGA TGACTGAGAA GTTCCAGATT GCTCAAGAGG AGAACTACCG CGATCCAACC 
AACATTCAGC AGAAACATCA GAAGCAACAA GCCTTCGAAG CCGAGCTTCA TGCCAATTCG 
GACAGAATCG CTGCTATCAT TCAAGCTGGA AACAACCTCA TTGAGAACGC CAAGTGCGGA 
GGAGGTGAAG CTGCTGTCAG CGCTCGACTG AAGGCTCTCA ACGACCAATG GGACCTTCTT 
GTCAAGACCA CCACCGAGAA GAGTTACCGT TTGAAAGAGG CTAACAAGCA GAAGAGCTTC 
ATGGCTGCTG TCAAGGATCT TGAGTTCTGG CTGGGAGAGG TTGAAATTCT CCTCCAATCT 
GAAGACTATG GAAAAGATTT GGCTTCCGTT GAAAACCTGC TCAAAAAACA TTCTCTTCTT 
GAGGCTGACA TTGTTGCCCA CCAGGATCGT GTTGGAGAGA TGAACGAGCA AGCTGATTCT 
CTTCTCGAGA ACGATCAATT CCAAGGACAA CAAATCGCTG AGAGAAGAAA GTTGATTGCT 
GATCGTTATG ATGGTGTCAA GAAGATGGCT ACCGATCGTC GTGATAAATT GAGCAAGGCC 
CTCAATGTTC ACCAGTTCTT CCGTGATATT GATGATGAGG AGTCTTGGAT TAAGGAGAAG 
AAGCTTCTCG TTTCTTCTGA TGACTATGGA CGTGATCTTC CAGGAGTTCA AAACCTTCGC 
CGCAAGCATC GTCGTATTGA TACCGAGCTC GCTTCCCATG AGCCTCAAGT TTCTCTTGTC 
AAGCTGAAGG GAGAAGAGCT GTTGAGATCT GCAGCCGAAG CGGGAGTTGG AGAGGATCAA 
ATTAAGAAGC GTATGGAGGA TCTTGAGCAA AGCTGGGGAC AAATCCGTGA TCTTACTGGA 
AACCGTCATC AACGCCTCGA CGAGTCTGAA GCCTTCCAAG CTTTCTTGGG TGATGTTGAA 
GAGGAAGAGG CATGGATGAA TGAGAAACAA CAAATCCTGG GATCCGACAA CTTTGGAGAA 
AACATGGCTG GAGTGCAAGG ACTTCTCAAG AAGCACGATA CGTTCCAAGT CGATCTTGAG 
CTTCACAAGC AACGTGTTGC TGATTTGATC AACAAGGGAG ACACTCTTAT TCACAATGGA 
AACCATCATG CTCCTCATAT CAAGCAGAGA TGCGACCAAC TCCGTGCTCG TCTTATTGAG 
ATTGAGAGCA TGGCCGAAGG ACGTCTTGCC AAGCTTCGTG ATAACTCTGC CTACCTTCAA 
TTCATGTGGA AGTGTGATGT TGTGGAATCG TGGATTGCTG AGAAGGAGCA ACAAGTTCGT 
TCCGAAGACT TTGGAAGAGA TCTCTCATCT GTTCAAATCC TCCTCACCAA GCAAGAAGCT 
TTCGACGCTG GACTCAACGC TTTCGAACAT GAAGGAATTC AAAGAATCAC CGAGCTCAAA 
GACCAACTTG TTTCGAGTGA GCACCAACAA TCTCCAGCCA TTGAGAAACG TCATACGAAC 
GTCATCCAAC GTTGGCAGAA CCTTCTTGCC CACTCCGAGG CTCGTCGTCA GAAGTTGTTG 
AAAATGCAAC AGCAATTCAA GCAAATCGAG GAGCTTTACC TTGCCTTTGC CAAGAAGGCT 
TCCACTTTCA ACTCATGGTT CGAGAACGCT GAGGAGGACT TGACTGACCC TGTCCGATGC 
AACTCTTTGG AAGAGATCCG TGCCTTGCGC GACGCCCATG GTGAGTTCCA GCGCTCATTG 
TCCTCCGCTG AAGAGGACTT CCGCCAACTC CAAGACCTTG ATCGTCGGAT CAAGTCGTTC 
AATGTGGGAC CCAACCCTTA CACGTGGTTC ACTATGGATG CCCTCGAAGA CACGTGGCGC 
AACCTGCAGA GAATCATCAA GGAGCGCGAG CAGGAGCTTG CCAGAGAGCA CCAAAGACAG 
GAGGAGAACG ACAAATTGCG ACGCGAGTTT GCCAAGTTGG CCAATGCTTT CCATGCTTGG 
TTGACAAATA CCAGACAGGA GATGATGGAA GCTGGAGGTA CATTGGAAGA ACAACTCGAT 
GCTGTTGAGA GGAAAGCAAA GGAAATCAAG GCGAACAAAG GTCAGCTGCG GCAGATTGAA 
GAGAAGGGTG CTATGTTGGA GAGAAACCTT ATCCTAGACA ATAGGTATAC TGAACATTCG 
ACAGTAGGAA TTGCGCAAGC TTGGGATCAA TTGGATCAGC TAGCCATGCG AATGCAACAC 
AATTTGGAAC AACAGATTCA AGCCAGAAAT CAATCTGGAG TATCGGAAGA AGCCCTTCGT 
GAGTTCTCAA TGATGTTCAA GCACTTCGAC AAGGAGAAAA CTGGAAGACT CGACCACCAG 
CAGTTCAAGA GTTGTCTGCG AGCTCTTGGA TACGATCTGC CGATGGTGGA CGAAGGCCAA 
CCAGAACCCG AGTTCCAGAG AATTTTGGAT ATTGTCGACC CGAACAGAGA TGGTTACGTA 
ACTCTGCAAG AGTACATGGC GTTCATGATC AGCAAAGAAA CCGAGAACAT CCAGTCTTCG 
GAAGAAATCG AGATGGCATT CAGAGCTCTT TCGAAGGAAT TCCGACCATA TGTCACGGCA 
GAGGAGCTCT ACGCGAACCT GACACCGGAG CAAGCCGAAT TCTGCATACG AAGAATGAAG 
CCATACATGG ACGCCATTTC AGGTCGTTCC ATCCAAGGTG GTCTCGACTA CGAGCAGTTT 
GTGCATGCCT TGTTCCAGAG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spc-1 ( NM_001270071.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001257000.1 Caenorhabditis elegans SPeCtrin (spc-1), mRNA.

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CloneID	OCa349365
Clone ID Related Accession (Same CDS sequence)	NM_001270071.3 , NM_001270071.1
Accession Version	NM_001270071.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7299bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	SPeCtrin
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Oct 18, 2019 this sequence version replaced NM_001270071.2.

ATGGCTGATT CGAATGACAC TCATGCTCCA CCAGAGCCGG TGCTAGAAGT ACCGCCACCA 
CAGGAGATTC GTATTCTGGA GACTGCTGAT GACATTCACC AACGTCGTCA GGAAGTATTA 
GGTCACTATT CAATTTTCAA GGATCACTCG CAGACAAAAC GAGATCGTCT CGAGGAAGCT 
CGTCAGTTTC AATATTTCAA ACGAGATGCT GATGAGTTGA ACGTTTGGAT TCTTGAAAAG 
CTCCAGACAG CTCAGGAAGA AAACTTCAGA GATCCGAGCA ATCTCCAGGC CAAGATCCAG 
AAGCATGAAG CGTTCGAAGC TGAGGTTCAA GCTCACGCTA AGACCATCGC CAACCTCGAC 
AAGACCGGAA ACGCTATGAT CCAACACAAT CATTTCGCTA GCGAACACAT CAAGAAACGC 
TTGGAAGAGT TGCACGCCCT CTGGGATAAG CTCTTCTTCA AACTGAAGGA TAAGGGAATT 
AAGCTACAAC AAGCACTTAA ACTTTTGCAT TTTATTCGTC AATGCGATGA AATTCTCTAC 
TGGATTCGAG AGAAGGAAAC CTATGTCACC GCAGAAGACA TGGGAATGGA TCTCGAACAC 
GTTGAGATAC TTCAGCGTAA ATTTGACGAC TTTTTGAAAG AACTCGGTAA CCATCAGTAC 
CGAATCAATG AGATCAATCA AGCTGCTGAC AAACTTGTCG AGGAGGGACA CTCTGAGCAC 
GATCAGATCT ACAAGAAGCG CGATGACGTT AATGATGCTT GGCATCGTTT GAACACTTTG 
GCGGCCACTC GTAAGGAAGG GCTCTTTGGA GCTCATCAAG TTCAACGCTT CAATCGCGAT 
GCAGACGAGA CCCTTGCATG GATTGGAGAG AAGGATCTTG CTTTGTCAAG CGATGATTAC 
GGAAGAGACC TCAATAATGT TCAAGCTCTT CAACGCAAGC ATGAAGGAAC TGAACGTGAT 
CTTGCTGCTC TTGAAGGAAA GATGCTCCAA TTGGAGAAAG AAGCTCATAA GTTGGCAGAA 
ACTCATCCAG ATCGTGCTGA AGCGATTCAT CAAAAGAACG ATGACACCAA AGCCGCCTGG 
AATGCACTCA AGGAGAAGGC TCAACACCGC AAAGATGGAC TTGAGAGAAG CTTCCAGCTT 
CATCGTTTCT TGGCCGACTA CCGTGACTTG ATTAGCTGGA TTGGAGACAT GAAAGCTGTC 
ATCGGAGCTG ATGAGCTTGC AAAAGACGTG GCTGGCGCTG AAGCTCTTCT TGAAAGTCAT 
CAAGAACACA AGGGAGAAAT TGATGCTCGT GCTGACTCGT TCAACCAAAC TGCATCCGCT 
GGACAAAAGC TCGTTGAGAT GGGAATTCCA GAGAGTCCAG AAGTTCAGGA GAAGCTCGAG 
AAACTTGACC ACGAGAAGTC TTCTCTTCTT GGATTGTGGG AGGAACGTCG TATTCTCTAC 
GAGCAATGTA TGGATCTCCA ACTCTTCTAT CGTGACACTG AACAAGCCGA AACCTGGATG 
AACAAGCAGG AAGCGTTCCT TGCCAACACT GATTTGGGAG ATTCACTAGA TTCTGTCGAG 
CACCTCATCA AGAAACACGA GGACTTTGAG AAATCGCTTG CTGCTCAAGA GGAGAAGATC 
AATGCACTTG ACGAGTTTGC TACCAAGCTC ATTCAAGGAC AGCATTACGC TGCTGATGAT 
GTTGCCGAGA GACGCCAAGC TCTTCTTGAC CGTCGTCGTC GTCTTCTAGA CAGAGCTCGT 
CAACGTGGAA ATGCTCTCAA GGAAAGCTAC AAGCGCCAGA CATTTGACCG TGACTGTGAT 
GAGATGGTTA GCTGGATCAC TGAAAAACTG TCCACCGCTC GTGACGATTC TTACTTGGAT 
CCTACCAACA TTCGTGGAAA ACTCCAAAAG CACATCAACT TTGAGCAAGA GCTTAAGGCT 
AACGAGAACC GTTTGGACGA TATTCGTTCT ACCGGAGAGC AAATCATCGA TAGTGGACAC 
TTTGCTGCTG ATCATATCGG AGACCGCCTT CGTCAAGTCA ACAACTTGTG GAACGAATTG 
GTCGATGCTA CAAACAAGAA GGGAGCCAAA CTTCGTGAAG CTGGAAACGA ACAGCAATTC 
AATCGCAACA TTGAAGATGT TGAAGCATGG TTGTCGGAGC TTGAAGGACA AGTTGCTTCC 
GAGGATTATG GAAAAGATCT TGTATCTGTT CAAAACCTTC AGAAGAAGAT TGGACTTATT 
GAGTCTGATT TTAACGCTCA CAACGATCGT GTTGATGGAA TCAAGCAACT TGCTCAACAG 
TTCCAAGAGG AGGAGCATTT CAATGCTCCA GTCATTGTTC GCAAGCAAGA GAATCTTCAG 
CAGCGCTACA ACGCTCTCCG TGATCCACTT GAGAAGAGAA AAAGAAAACT CGGAGAGTCC 
CACCAAGGAA ATCAACTGTT CCGCGATATT GAAGATGAGC TGGCTTGGAT CCGTGAGAAG 
GAACAAGTTG CTGGATCAAC CAATCGAGGA CGTGATTTGA TTGGAGTTCA AAATCTCATC 
AAGAAGCAGC AGGCTCTGAT CGCTGAGATT GCCAACCACG ATAGCCAAAT CGAGAGTGTC 
AGCAGTGCCG CCAATGACAT GATTCAACAA GGACACTTCT TGGCTCCAGA GATACGTGAT 
AAACTTGCTC AACTTCGTGA CAACTGGAGA ATTCTGAAGA GCAAGGCTGA GAAGAGACGC 
ACAGATCTTG ATGACTCACT CCAAGCCCAC CAATACCTTT CTGATGCCAA TGAGGCCGAT 
GCTTGGATGA GCGAGAAAGA ACCAATTGTT GGATCCACCG ACTATGGAAA GGACGAAGAT 
TCTGCCGAGG CTCTTCTCAA GAAGCATCGT GCTCTTCTTT CCGACTTGGA AGCTTTCAAG 
GGAACTATCG AGGATCTTCG CAAGCAAGCT TCTCAATGCA AGTACCAAGA GCAACCGATG 
GGACAACTCG GACGTGACTG TGTGTTGGCT CTTTACGATT ACCAAGAGAA ATCTCCTCGT 
GAGGTTTCCA TGAAGAAGGG AGATGTTCTC ACTCTTCTCA ATGCTAGCAA CAGAGACTGG 
TGGAAAGTTG AGGTCAATGA TCGTCAAGGA TTTGTTCCAG CTGCCTACGT TAAGAGAATC 
GAACCAGGAA CTGCTCAACA GCATGCTCAA CAACAGGTCA ACTCCATTGG AGGAAAGCAG 
TCGGAGATTG AGGACAAGTA CCAACGTCTC ATGATGCTCG GAGAGACTAG AAAGAGAAAG 
CTCGAGGAAG CTTGCAAAGG ATACCAACTT CTCCGTGAAG CCAACGACTT GGCTGAATGG 
ATCAAGTCTA GAACTAACAT GGCAGAAGAG GCTGTTGCTG CTCAACAAGA AATTGGAACT 
GACCTCGAGC AGGTTGAGGT TCTTCAAAAG AAGTTTGACG ACTTTAAAGG AGACCTCAAG 
GCTAACGAGG TTCGTCTCCA GGAGATGAAC CAAATTGCCA CCGCTCTCAC ATCGGTTGGA 
CAAACTGAAA CTGCTGTTCG TATTCGTCAG CAAATTGAGG ATCTCAACGC CAGATGGCGC 
GCATTGGAGG AGCAGACTGA GCAACGCGAA CAACAACTCG GATCTGCCCA CGAGGTGCAA 
CGCTTCCACC GTGATGTCGA CGAGACTCGT GATTGGATCC AGGAGAAGGA TGATGCCCTT 
GACAGTGAAG ATTTTGGACG TGACCTCCGT TCCGTTCAGG CTCTTCAACG CAAGCATGAA 
GGAGTTGAGC GTGATTTGGC TGCTCTTGGA GACAAGATTA AATCTCTTGA TGAGAAGGCC 
AACCGTCTTC GTCAATCTCA CCCAGAAGCT GCCGAGCAGA TTTACGATTT GCAACGTGAG 
CTCAATGAGC AATGGAACCG CCTCACGTCT AAGGCCAACA ACCGAAAAGA AAAGCTTCTT 
GATTCGTACG ACTACCAACG CTTTTTGTCT GACTACCGTG ATTTGATGCA ATGGATTGCT 
TCCATGAACC AACTTGTCAG CAGTCAAGAA CTTGCCAACG ATGTTACTGG AGCTGAGGCT 
CTTCTTGAGC GCCACCAGGA GTACCGCACC GAAATTGACT CCCGTGCTGC CACCTTCCAA 
GCTTTTGATC AATTTGGAAA CCAACTTCTT AACAGCCACC ACTACGCTGC AGGAGACATT 
GAAAACCGTC TTCAAGGTGT CAACGAGGCT CGTAAAGGAC TTGAAGACGC ATGGGTTGCT 
CGTCGTAACA TTCTCGATCA ATGCCTCGAA CTTCAACTCT TCTACCGTGA TTGTGAGCAA 
GCCGACACTT GGATGTCAGC TCGTGAAGCT TTCTTGGCTC AAGAGGATCC ATCTGGAGAC 
AATGTTGAGT CTTTGATCAA GAAACACGAG GACTTTGACA AAGCCATCAA CACTCAAGAG 
GAGAAAATCA AGGGACTCAA GCTCTTTGCT GAATCTCTCA TCAAGAACAA TCATTACGAT 
TCGCCAGCAG TCACTCGCAA GAGAGACCAG ATTCTCGATA GATGGAATGG TCTTAAGGAG 
GCTCTCATTC AGAAGAGATT CAAGCTTGGA GAGTCACAAA CTCTTCAACA GTTTAGTCGT 
GATGCGGATG AGATTGAGAA CTGGATGACT GAGAAGTTCC AGATTGCTCA AGAGGAGAAC 
TACCGCGATC CAACCAACAT TCAGCAGAAA CATCAGAAGC AACAAGCCTT CGAAGCCGAG 
CTTCATGCCA ATTCGGACAG AATCGCTGCT ATCATTCAAG CTGGAAACAA CCTCATTGAG 
AACGCCAAGT GCGGAGGAGG TGAAGCTGCT GTCAGCGCTC GACTGAAGGC TCTCAACGAC 
CAATGGGACC TTCTTGTCAA GACCACCACC GAGAAGAGTT ACCGTTTGAA AGAGGCTAAC 
AAGCAGAAGA GCTTCATGGC TGCTGTCAAG GATCTTGAGT TCTGGCTGGG AGAGGTTGAA 
ATTCTCCTCC AATCTGAAGA CTATGGAAAA GATTTGGCTT CCGTTGAAAA CCTGCTCAAA 
AAACATTCTC TTCTTGAGGC TGACATTGTT GCCCACCAGG ATCGTGTTGG AGAGATGAAC 
GAGCAAGCTG ATTCTCTTCT CGAGAACGAT CAATTCCAAG GACAACAAAT CGCTGAGAGA 
AGAAAGTTGA TTGCTGATCG TTATGATGGT GTCAAGAAGA TGGCTACCGA TCGTCGTGAT 
AAATTGAGCA AGGCCCTCAA TGTTCACCAG TTCTTCCGTG ATATTGATGA TGAGGAGTCT 
TGGATTAAGG AGAAGAAGCT TCTCGTTTCT TCTGATGACT ATGGACGTGA TCTTCCAGGA 
GTTCAAAACC TTCGCCGCAA GCATCGTCGT ATTGATACCG AGCTCGCTTC CCATGAGCCT 
CAAGTTTCTC TTGTCAAGCT GAAGGGAGAA GAGCTGTTGA GATCTGCAGC CGAAGCGGGA 
GTTGGAGAGG ATCAAATTAA GAAGCGTATG GAGGATCTTG AGCAAAGCTG GGGACAAATC 
CGTGATCTTA CTGGAAACCG TCATCAACGC CTCGACGAGT CTGAAGCCTT CCAAGCTTTC 
TTGGGTGATG TTGAAGAGGA AGAGGCATGG ATGAATGAGA AACAACAAAT CCTGGGATCC 
GACAACTTTG GAGAAAACAT GGCTGGAGTG CAAGGACTTC TCAAGAAGCA CGATACGTTC 
CAAGTCGATC TTGAGCTTCA CAAGCAACGT GTTGCTGATT TGATCAACAA GGGAGACACT 
CTTATTCACA ATGGAAACCA TCATGCTCCT CATATCAAGC AGAGATGCGA CCAACTCCGT 
GCTCGTCTTA TTGAGATTGA GAGCATGGCC GAAGGACGTC TTGCCAAGCT TCGTGATAAC 
TCTGCCTACC TTCAATTCAT GTGGAAGTGT GATGTTGTGG AATCGTGGAT TGCTGAGAAG 
GAGCAACAAG TTCGTTCCGA AGACTTTGGA AGAGATCTCT CATCTGTTCA AATCCTCCTC 
ACCAAGCAAG AAGCTTTCGA CGCTGGACTC AACGCTTTCG AACATGAAGG AATTCAAAGA 
ATCACCGAGC TCAAAGACCA ACTTGTTTCG AGTGAGCACC AACAATCTCC AGCCATTGAG 
AAACGTCATA CGAACGTCAT CCAACGTTGG CAGAACCTTC TTGCCCACTC CGAGGCTCGT 
CGTCAGAAGT TGTTGAAAAT GCAACAGCAA TTCAAGCAAA TCGAGGAGCT TTACCTTGCC 
TTTGCCAAGA AGGCTTCCAC TTTCAACTCA TGGTTCGAGA ACGCTGAGGA GGACTTGACT 
GACCCTGTCC GATGCAACTC TTTGGAAGAG ATCCGTGCCT TGCGCGACGC CCATGGTGAG 
TTCCAGCGCT CATTGTCCTC CGCTGAAGAG GACTTCCGCC AACTCCAAGA CCTTGATCGT 
CGGATCAAGT CGTTCAATGT GGGACCCAAC CCTTACACGT GGTTCACTAT GGATGCCCTC 
GAAGACACGT GGCGCAACCT GCAGAGAATC ATCAAGGAGC GCGAGCAGGA GCTTGCCAGA 
GAGCACCAAA GACAGGAGGA GAACGACAAA TTGCGACGCG AGTTTGCCAA GTTGGCCAAT 
GCTTTCCATG CTTGGTTGAC AAATACCAGA CAGGAGATGA TGGAAGCTGG AGGTACATTG 
GAAGAACAAC TCGATGCTGT TGAGAGGAAA GCAAAGGAAA TCAAGGCGAA CAAAGGTCAG 
CTGCGGCAGA TTGAAGAGAA GGGTGCTATG TTGGAGAGAA ACCTTATCCT AGACAATAGG 
TATACTGAAC ATTCGACAGT AGGAATTGCG CAAGCTTGGG ATCAATTGGA TCAGCTAGCC 
ATGCGAATGC AACACAATTT GGAACAACAG ATTCAAGCCA GAAATCAATC TGGAGTATCG 
GAAGAAGCCC TTCGTGAGTT CTCAATGATG TTCAAGCACT TCGACAAGGA GAAAACTGGA 
AGACTCGACC ACCAGCAGTT CAAGAGTTGT CTGCGAGCTC TTGGATACGA TCTGCCGATG 
GTGGACGAAG GCCAACCAGA ACCCGAGTTC CAGAGAATTT TGGATATTGT CGACCCGAAC 
AGAGATGGTT ACGTAACTCT GCAAGAGTAC ATGGCGTTCA TGATCAGCAA AGAAACCGAG 
AACATCCAGT CTTCGGAAGA AATCGAGATG GCATTCAGAG CTCTTTCGAA GGAATTCCGA 
CCATATGTCA CGGCAGAGGA GCTCTACGCG AACCTGACAC CGGAGCAAGC CGAATTCTGC 
ATACGAAGAA TGAAGCCATA CATGGACGCC ATTTCAGGTC GTTCCATCCA AGGTGGTCTC 
GACTACGAGC AGTTTGTGCA TGCCTTGTTC CAGAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001257000.1
CDS	13..7311
Translation	MADSNDTHAPPEPVLEVPPPQEIRILETADDIHQRRQEVLGHYSIFKDHSQTKRDRLEEARQFQYFKRDADELNVWILEKLQTAQEENFRDPSNLQAKIQKHEAFEAEVQAHAKTIANLDKTGNAMIQHNHFASEHIKKRLEELHALWDKLFFKLKDKGIKLQQALKLLHFIRQCDEILYWIREKETYVTAEDMGMDLEHVEILQRKFDDFLKELGNHQYRINEINQAADKLVEEGHSEHDQIYKKRDDVNDAWHRLNTLAATRKEGLFGAHQVQRFNRDADETLAWIGEKDLALSSDDYGRDLNNVQALQRKHEGTERDLAALEGKMLQLEKEAHKLAETHPDRAEAIHQKNDDTKAAWNALKEKAQHRKDGLERSFQLHRFLADYRDLISWIGDMKAVIGADELAKDVAGAEALLESHQEHKGEIDARADSFNQTASAGQKLVEMGIPESPEVQEKLEKLDHEKSSLLGLWEERRILYEQCMDLQLFYRDTEQAETWMNKQEAFLANTDLGDSLDSVEHLIKKHEDFEKSLAAQEEKINALDEFATKLIQGQHYAADDVAERRQALLDRRRRLLDRARQRGNALKESYKRQTFDRDCDEMVSWITEKLSTARDDSYLDPTNIRGKLQKHINFEQELKANENRLDDIRSTGEQIIDSGHFAADHIGDRLRQVNNLWNELVDATNKKGAKLREAGNEQQFNRNIEDVEAWLSELEGQVASEDYGKDLVSVQNLQKKIGLIESDFNAHNDRVDGIKQLAQQFQEEEHFNAPVIVRKQENLQQRYNALRDPLEKRKRKLGESHQGNQLFRDIEDELAWIREKEQVAGSTNRGRDLIGVQNLIKKQQALIAEIANHDSQIESVSSAANDMIQQGHFLAPEIRDKLAQLRDNWRILKSKAEKRRTDLDDSLQAHQYLSDANEADAWMSEKEPIVGSTDYGKDEDSAEALLKKHRALLSDLEAFKGTIEDLRKQASQCKYQEQPMGQLGRDCVLALYDYQEKSPREVSMKKGDVLTLLNASNRDWWKVEVNDRQGFVPAAYVKRIEPGTAQQHAQQQVNSIGGKQSEIEDKYQRLMMLGETRKRKLEEACKGYQLLREANDLAEWIKSRTNMAEEAVAAQQEIGTDLEQVEVLQKKFDDFKGDLKANEVRLQEMNQIATALTSVGQTETAVRIRQQIEDLNARWRALEEQTEQREQQLGSAHEVQRFHRDVDETRDWIQEKDDALDSEDFGRDLRSVQALQRKHEGVERDLAALGDKIKSLDEKANRLRQSHPEAAEQIYDLQRELNEQWNRLTSKANNRKEKLLDSYDYQRFLSDYRDLMQWIASMNQLVSSQELANDVTGAEALLERHQEYRTEIDSRAATFQAFDQFGNQLLNSHHYAAGDIENRLQGVNEARKGLEDAWVARRNILDQCLELQLFYRDCEQADTWMSAREAFLAQEDPSGDNVESLIKKHEDFDKAINTQEEKIKGLKLFAESLIKNNHYDSPAVTRKRDQILDRWNGLKEALIQKRFKLGESQTLQQFSRDADEIENWMTEKFQIAQEENYRDPTNIQQKHQKQQAFEAELHANSDRIAAIIQAGNNLIENAKCGGGEAAVSARLKALNDQWDLLVKTTTEKSYRLKEANKQKSFMAAVKDLEFWLGEVEILLQSEDYGKDLASVENLLKKHSLLEADIVAHQDRVGEMNEQADSLLENDQFQGQQIAERRKLIADRYDGVKKMATDRRDKLSKALNVHQFFRDIDDEESWIKEKKLLVSSDDYGRDLPGVQNLRRKHRRIDTELASHEPQVSLVKLKGEELLRSAAEAGVGEDQIKKRMEDLEQSWGQIRDLTGNRHQRLDESEAFQAFLGDVEEEEAWMNEKQQILGSDNFGENMAGVQGLLKKHDTFQVDLELHKQRVADLINKGDTLIHNGNHHAPHIKQRCDQLRARLIEIESMAEGRLAKLRDNSAYLQFMWKCDVVESWIAEKEQQVRSEDFGRDLSSVQILLTKQEAFDAGLNAFEHEGIQRITELKDQLVSSEHQQSPAIEKRHTNVIQRWQNLLAHSEARRQKLLKMQQQFKQIEELYLAFAKKASTFNSWFENAEEDLTDPVRCNSLEEIRALRDAHGEFQRSLSSAEEDFRQLQDLDRRIKSFNVGPNPYTWFTMDALEDTWRNLQRIIKEREQELAREHQRQEENDKLRREFAKLANAFHAWLTNTRQEMMEAGGTLEEQLDAVERKAKEIKANKGQLRQIEEKGAMLERNLILDNRYTEHSTVGIAQAWDQLDQLAMRMQHNLEQQIQARNQSGVSEEALREFSMMFKHFDKEKTGRLDHQQFKSCLRALGYDLPMVDEGQPEPEFQRILDIVDPNRDGYVTLQEYMAFMISKETENIQSSEEIEMAFRALSKEFRPYVTAEELYANLTPEQAEFCIRRMKPYMDAISGRSIQGGLDYEQFVHALFQS

Target ORF information:

RefSeq Version	NM_001270071.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans SPeCtrin (spc-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001270071.3

ORF Insert Sequence:

ATGGCTGATT CGAATGACAC TCATGCTCCA CCAGAGCCGG TGCTAGAAGT ACCGCCACCA 
CAGGAGATTC GTATTCTGGA GACTGCTGAT GACATTCACC AACGTCGTCA GGAAGTATTA 
GGTCACTATT CAATTTTCAA GGATCACTCG CAGACAAAAC GAGATCGTCT CGAGGAAGCT 
CGTCAGTTTC AATATTTCAA ACGAGATGCT GATGAGTTGA ACGTTTGGAT TCTTGAAAAG 
CTCCAGACAG CTCAGGAAGA AAACTTCAGA GATCCGAGCA ATCTCCAGGC CAAGATCCAG 
AAGCATGAAG CGTTCGAAGC TGAGGTTCAA GCTCACGCTA AGACCATCGC CAACCTCGAC 
AAGACCGGAA ACGCTATGAT CCAACACAAT CATTTCGCTA GCGAACACAT CAAGAAACGC 
TTGGAAGAGT TGCACGCCCT CTGGGATAAG CTCTTCTTCA AACTGAAGGA TAAGGGAATT 
AAGCTACAAC AAGCACTTAA ACTTTTGCAT TTTATTCGTC AATGCGATGA AATTCTCTAC 
TGGATTCGAG AGAAGGAAAC CTATGTCACC GCAGAAGACA TGGGAATGGA TCTCGAACAC 
GTTGAGATAC TTCAGCGTAA ATTTGACGAC TTTTTGAAAG AACTCGGTAA CCATCAGTAC 
CGAATCAATG AGATCAATCA AGCTGCTGAC AAACTTGTCG AGGAGGGACA CTCTGAGCAC 
GATCAGATCT ACAAGAAGCG CGATGACGTT AATGATGCTT GGCATCGTTT GAACACTTTG 
GCGGCCACTC GTAAGGAAGG GCTCTTTGGA GCTCATCAAG TTCAACGCTT CAATCGCGAT 
GCAGACGAGA CCCTTGCATG GATTGGAGAG AAGGATCTTG CTTTGTCAAG CGATGATTAC 
GGAAGAGACC TCAATAATGT TCAAGCTCTT CAACGCAAGC ATGAAGGAAC TGAACGTGAT 
CTTGCTGCTC TTGAAGGAAA GATGCTCCAA TTGGAGAAAG AAGCTCATAA GTTGGCAGAA 
ACTCATCCAG ATCGTGCTGA AGCGATTCAT CAAAAGAACG ATGACACCAA AGCCGCCTGG 
AATGCACTCA AGGAGAAGGC TCAACACCGC AAAGATGGAC TTGAGAGAAG CTTCCAGCTT 
CATCGTTTCT TGGCCGACTA CCGTGACTTG ATTAGCTGGA TTGGAGACAT GAAAGCTGTC 
ATCGGAGCTG ATGAGCTTGC AAAAGACGTG GCTGGCGCTG AAGCTCTTCT TGAAAGTCAT 
CAAGAACACA AGGGAGAAAT TGATGCTCGT GCTGACTCGT TCAACCAAAC TGCATCCGCT 
GGACAAAAGC TCGTTGAGAT GGGAATTCCA GAGAGTCCAG AAGTTCAGGA GAAGCTCGAG 
AAACTTGACC ACGAGAAGTC TTCTCTTCTT GGATTGTGGG AGGAACGTCG TATTCTCTAC 
GAGCAATGTA TGGATCTCCA ACTCTTCTAT CGTGACACTG AACAAGCCGA AACCTGGATG 
AACAAGCAGG AAGCGTTCCT TGCCAACACT GATTTGGGAG ATTCACTAGA TTCTGTCGAG 
CACCTCATCA AGAAACACGA GGACTTTGAG AAATCGCTTG CTGCTCAAGA GGAGAAGATC 
AATGCACTTG ACGAGTTTGC TACCAAGCTC ATTCAAGGAC AGCATTACGC TGCTGATGAT 
GTTGCCGAGA GACGCCAAGC TCTTCTTGAC CGTCGTCGTC GTCTTCTAGA CAGAGCTCGT 
CAACGTGGAA ATGCTCTCAA GGAAAGCTAC AAGCGCCAGA CATTTGACCG TGACTGTGAT 
GAGATGGTTA GCTGGATCAC TGAAAAACTG TCCACCGCTC GTGACGATTC TTACTTGGAT 
CCTACCAACA TTCGTGGAAA ACTCCAAAAG CACATCAACT TTGAGCAAGA GCTTAAGGCT 
AACGAGAACC GTTTGGACGA TATTCGTTCT ACCGGAGAGC AAATCATCGA TAGTGGACAC 
TTTGCTGCTG ATCATATCGG AGACCGCCTT CGTCAAGTCA ACAACTTGTG GAACGAATTG 
GTCGATGCTA CAAACAAGAA GGGAGCCAAA CTTCGTGAAG CTGGAAACGA ACAGCAATTC 
AATCGCAACA TTGAAGATGT TGAAGCATGG TTGTCGGAGC TTGAAGGACA AGTTGCTTCC 
GAGGATTATG GAAAAGATCT TGTATCTGTT CAAAACCTTC AGAAGAAGAT TGGACTTATT 
GAGTCTGATT TTAACGCTCA CAACGATCGT GTTGATGGAA TCAAGCAACT TGCTCAACAG 
TTCCAAGAGG AGGAGCATTT CAATGCTCCA GTCATTGTTC GCAAGCAAGA GAATCTTCAG 
CAGCGCTACA ACGCTCTCCG TGATCCACTT GAGAAGAGAA AAAGAAAACT CGGAGAGTCC 
CACCAAGGAA ATCAACTGTT CCGCGATATT GAAGATGAGC TGGCTTGGAT CCGTGAGAAG 
GAACAAGTTG CTGGATCAAC CAATCGAGGA CGTGATTTGA TTGGAGTTCA AAATCTCATC 
AAGAAGCAGC AGGCTCTGAT CGCTGAGATT GCCAACCACG ATAGCCAAAT CGAGAGTGTC 
AGCAGTGCCG CCAATGACAT GATTCAACAA GGACACTTCT TGGCTCCAGA GATACGTGAT 
AAACTTGCTC AACTTCGTGA CAACTGGAGA ATTCTGAAGA GCAAGGCTGA GAAGAGACGC 
ACAGATCTTG ATGACTCACT CCAAGCCCAC CAATACCTTT CTGATGCCAA TGAGGCCGAT 
GCTTGGATGA GCGAGAAAGA ACCAATTGTT GGATCCACCG ACTATGGAAA GGACGAAGAT 
TCTGCCGAGG CTCTTCTCAA GAAGCATCGT GCTCTTCTTT CCGACTTGGA AGCTTTCAAG 
GGAACTATCG AGGATCTTCG CAAGCAAGCT TCTCAATGCA AGTACCAAGA GCAACCGATG 
GGACAACTCG GACGTGACTG TGTGTTGGCT CTTTACGATT ACCAAGAGAA ATCTCCTCGT 
GAGGTTTCCA TGAAGAAGGG AGATGTTCTC ACTCTTCTCA ATGCTAGCAA CAGAGACTGG 
TGGAAAGTTG AGGTCAATGA TCGTCAAGGA TTTGTTCCAG CTGCCTACGT TAAGAGAATC 
GAACCAGGAA CTGCTCAACA GCATGCTCAA CAACAGGTCA ACTCCATTGG AGGAAAGCAG 
TCGGAGATTG AGGACAAGTA CCAACGTCTC ATGATGCTCG GAGAGACTAG AAAGAGAAAG 
CTCGAGGAAG CTTGCAAAGG ATACCAACTT CTCCGTGAAG CCAACGACTT GGCTGAATGG 
ATCAAGTCTA GAACTAACAT GGCAGAAGAG GCTGTTGCTG CTCAACAAGA AATTGGAACT 
GACCTCGAGC AGGTTGAGGT TCTTCAAAAG AAGTTTGACG ACTTTAAAGG AGACCTCAAG 
GCTAACGAGG TTCGTCTCCA GGAGATGAAC CAAATTGCCA CCGCTCTCAC ATCGGTTGGA 
CAAACTGAAA CTGCTGTTCG TATTCGTCAG CAAATTGAGG ATCTCAACGC CAGATGGCGC 
GCATTGGAGG AGCAGACTGA GCAACGCGAA CAACAACTCG GATCTGCCCA CGAGGTGCAA 
CGCTTCCACC GTGATGTCGA CGAGACTCGT GATTGGATCC AGGAGAAGGA TGATGCCCTT 
GACAGTGAAG ATTTTGGACG TGACCTCCGT TCCGTTCAGG CTCTTCAACG CAAGCATGAA 
GGAGTTGAGC GTGATTTGGC TGCTCTTGGA GACAAGATTA AATCTCTTGA TGAGAAGGCC 
AACCGTCTTC GTCAATCTCA CCCAGAAGCT GCCGAGCAGA TTTACGATTT GCAACGTGAG 
CTCAATGAGC AATGGAACCG CCTCACGTCT AAGGCCAACA ACCGAAAAGA AAAGCTTCTT 
GATTCGTACG ACTACCAACG CTTTTTGTCT GACTACCGTG ATTTGATGCA ATGGATTGCT 
TCCATGAACC AACTTGTCAG CAGTCAAGAA CTTGCCAACG ATGTTACTGG AGCTGAGGCT 
CTTCTTGAGC GCCACCAGGA GTACCGCACC GAAATTGACT CCCGTGCTGC CACCTTCCAA 
GCTTTTGATC AATTTGGAAA CCAACTTCTT AACAGCCACC ACTACGCTGC AGGAGACATT 
GAAAACCGTC TTCAAGGTGT CAACGAGGCT CGTAAAGGAC TTGAAGACGC ATGGGTTGCT 
CGTCGTAACA TTCTCGATCA ATGCCTCGAA CTTCAACTCT TCTACCGTGA TTGTGAGCAA 
GCCGACACTT GGATGTCAGC TCGTGAAGCT TTCTTGGCTC AAGAGGATCC ATCTGGAGAC 
AATGTTGAGT CTTTGATCAA GAAACACGAG GACTTTGACA AAGCCATCAA CACTCAAGAG 
GAGAAAATCA AGGGACTCAA GCTCTTTGCT GAATCTCTCA TCAAGAACAA TCATTACGAT 
TCGCCAGCAG TCACTCGCAA GAGAGACCAG ATTCTCGATA GATGGAATGG TCTTAAGGAG 
GCTCTCATTC AGAAGAGATT CAAGCTTGGA GAGTCACAAA CTCTTCAACA GTTTAGTCGT 
GATGCGGATG AGATTGAGAA CTGGATGACT GAGAAGTTCC AGATTGCTCA AGAGGAGAAC 
TACCGCGATC CAACCAACAT TCAGCAGAAA CATCAGAAGC AACAAGCCTT CGAAGCCGAG 
CTTCATGCCA ATTCGGACAG AATCGCTGCT ATCATTCAAG CTGGAAACAA CCTCATTGAG 
AACGCCAAGT GCGGAGGAGG TGAAGCTGCT GTCAGCGCTC GACTGAAGGC TCTCAACGAC 
CAATGGGACC TTCTTGTCAA GACCACCACC GAGAAGAGTT ACCGTTTGAA AGAGGCTAAC 
AAGCAGAAGA GCTTCATGGC TGCTGTCAAG GATCTTGAGT TCTGGCTGGG AGAGGTTGAA 
ATTCTCCTCC AATCTGAAGA CTATGGAAAA GATTTGGCTT CCGTTGAAAA CCTGCTCAAA 
AAACATTCTC TTCTTGAGGC TGACATTGTT GCCCACCAGG ATCGTGTTGG AGAGATGAAC 
GAGCAAGCTG ATTCTCTTCT CGAGAACGAT CAATTCCAAG GACAACAAAT CGCTGAGAGA 
AGAAAGTTGA TTGCTGATCG TTATGATGGT GTCAAGAAGA TGGCTACCGA TCGTCGTGAT 
AAATTGAGCA AGGCCCTCAA TGTTCACCAG TTCTTCCGTG ATATTGATGA TGAGGAGTCT 
TGGATTAAGG AGAAGAAGCT TCTCGTTTCT TCTGATGACT ATGGACGTGA TCTTCCAGGA 
GTTCAAAACC TTCGCCGCAA GCATCGTCGT ATTGATACCG AGCTCGCTTC CCATGAGCCT 
CAAGTTTCTC TTGTCAAGCT GAAGGGAGAA GAGCTGTTGA GATCTGCAGC CGAAGCGGGA 
GTTGGAGAGG ATCAAATTAA GAAGCGTATG GAGGATCTTG AGCAAAGCTG GGGACAAATC 
CGTGATCTTA CTGGAAACCG TCATCAACGC CTCGACGAGT CTGAAGCCTT CCAAGCTTTC 
TTGGGTGATG TTGAAGAGGA AGAGGCATGG ATGAATGAGA AACAACAAAT CCTGGGATCC 
GACAACTTTG GAGAAAACAT GGCTGGAGTG CAAGGACTTC TCAAGAAGCA CGATACGTTC 
CAAGTCGATC TTGAGCTTCA CAAGCAACGT GTTGCTGATT TGATCAACAA GGGAGACACT 
CTTATTCACA ATGGAAACCA TCATGCTCCT CATATCAAGC AGAGATGCGA CCAACTCCGT 
GCTCGTCTTA TTGAGATTGA GAGCATGGCC GAAGGACGTC TTGCCAAGCT TCGTGATAAC 
TCTGCCTACC TTCAATTCAT GTGGAAGTGT GATGTTGTGG AATCGTGGAT TGCTGAGAAG 
GAGCAACAAG TTCGTTCCGA AGACTTTGGA AGAGATCTCT CATCTGTTCA AATCCTCCTC 
ACCAAGCAAG AAGCTTTCGA CGCTGGACTC AACGCTTTCG AACATGAAGG AATTCAAAGA 
ATCACCGAGC TCAAAGACCA ACTTGTTTCG AGTGAGCACC AACAATCTCC AGCCATTGAG 
AAACGTCATA CGAACGTCAT CCAACGTTGG CAGAACCTTC TTGCCCACTC CGAGGCTCGT 
CGTCAGAAGT TGTTGAAAAT GCAACAGCAA TTCAAGCAAA TCGAGGAGCT TTACCTTGCC 
TTTGCCAAGA AGGCTTCCAC TTTCAACTCA TGGTTCGAGA ACGCTGAGGA GGACTTGACT 
GACCCTGTCC GATGCAACTC TTTGGAAGAG ATCCGTGCCT TGCGCGACGC CCATGGTGAG 
TTCCAGCGCT CATTGTCCTC CGCTGAAGAG GACTTCCGCC AACTCCAAGA CCTTGATCGT 
CGGATCAAGT CGTTCAATGT GGGACCCAAC CCTTACACGT GGTTCACTAT GGATGCCCTC 
GAAGACACGT GGCGCAACCT GCAGAGAATC ATCAAGGAGC GCGAGCAGGA GCTTGCCAGA 
GAGCACCAAA GACAGGAGGA GAACGACAAA TTGCGACGCG AGTTTGCCAA GTTGGCCAAT 
GCTTTCCATG CTTGGTTGAC AAATACCAGA CAGGAGATGA TGGAAGCTGG AGGTACATTG 
GAAGAACAAC TCGATGCTGT TGAGAGGAAA GCAAAGGAAA TCAAGGCGAA CAAAGGTCAG 
CTGCGGCAGA TTGAAGAGAA GGGTGCTATG TTGGAGAGAA ACCTTATCCT AGACAATAGG 
TATACTGAAC ATTCGACAGT AGGAATTGCG CAAGCTTGGG ATCAATTGGA TCAGCTAGCC 
ATGCGAATGC AACACAATTT GGAACAACAG ATTCAAGCCA GAAATCAATC TGGAGTATCG 
GAAGAAGCCC TTCGTGAGTT CTCAATGATG TTCAAGCACT TCGACAAGGA GAAAACTGGA 
AGACTCGACC ACCAGCAGTT CAAGAGTTGT CTGCGAGCTC TTGGATACGA TCTGCCGATG 
GTGGACGAAG GCCAACCAGA ACCCGAGTTC CAGAGAATTT TGGATATTGT CGACCCGAAC 
AGAGATGGTT ACGTAACTCT GCAAGAGTAC ATGGCGTTCA TGATCAGCAA AGAAACCGAG 
AACATCCAGT CTTCGGAAGA AATCGAGATG GCATTCAGAG CTCTTTCGAA GGAATTCCGA 
CCATATGTCA CGGCAGAGGA GCTCTACGCG AACCTGACAC CGGAGCAAGC CGAATTCTGC 
ATACGAAGAA TGAAGCCATA CATGGACGCC ATTTCAGGTC GTTCCATCCA AGGTGGTCTC 
GACTACGAGC AGTTTGTGCA TGCCTTGTTC CAGAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spc-1 ( NM_001270071.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001257000.1 Caenorhabditis elegans SPeCtrin (spc-1), partial mRNA.

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CloneID	OCa349365
Clone ID Related Accession (Same CDS sequence)	NM_001270071.3 , NM_001270071.1
Accession Version	NM_001270071.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7299bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	SPeCtrin
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). COMPLETENESS: incomplete on both ends.

ATGGCTGATT CGAATGACAC TCATGCTCCA CCAGAGCCGG TGCTAGAAGT ACCGCCACCA 
CAGGAGATTC GTATTCTGGA GACTGCTGAT GACATTCACC AACGTCGTCA GGAAGTATTA 
GGTCACTATT CAATTTTCAA GGATCACTCG CAGACAAAAC GAGATCGTCT CGAGGAAGCT 
CGTCAGTTTC AATATTTCAA ACGAGATGCT GATGAGTTGA ACGTTTGGAT TCTTGAAAAG 
CTCCAGACAG CTCAGGAAGA AAACTTCAGA GATCCGAGCA ATCTCCAGGC CAAGATCCAG 
AAGCATGAAG CGTTCGAAGC TGAGGTTCAA GCTCACGCTA AGACCATCGC CAACCTCGAC 
AAGACCGGAA ACGCTATGAT CCAACACAAT CATTTCGCTA GCGAACACAT CAAGAAACGC 
TTGGAAGAGT TGCACGCCCT CTGGGATAAG CTCTTCTTCA AACTGAAGGA TAAGGGAATT 
AAGCTACAAC AAGCACTTAA ACTTTTGCAT TTTATTCGTC AATGCGATGA AATTCTCTAC 
TGGATTCGAG AGAAGGAAAC CTATGTCACC GCAGAAGACA TGGGAATGGA TCTCGAACAC 
GTTGAGATAC TTCAGCGTAA ATTTGACGAC TTTTTGAAAG AACTCGGTAA CCATCAGTAC 
CGAATCAATG AGATCAATCA AGCTGCTGAC AAACTTGTCG AGGAGGGACA CTCTGAGCAC 
GATCAGATCT ACAAGAAGCG CGATGACGTT AATGATGCTT GGCATCGTTT GAACACTTTG 
GCGGCCACTC GTAAGGAAGG GCTCTTTGGA GCTCATCAAG TTCAACGCTT CAATCGCGAT 
GCAGACGAGA CCCTTGCATG GATTGGAGAG AAGGATCTTG CTTTGTCAAG CGATGATTAC 
GGAAGAGACC TCAATAATGT TCAAGCTCTT CAACGCAAGC ATGAAGGAAC TGAACGTGAT 
CTTGCTGCTC TTGAAGGAAA GATGCTCCAA TTGGAGAAAG AAGCTCATAA GTTGGCAGAA 
ACTCATCCAG ATCGTGCTGA AGCGATTCAT CAAAAGAACG ATGACACCAA AGCCGCCTGG 
AATGCACTCA AGGAGAAGGC TCAACACCGC AAAGATGGAC TTGAGAGAAG CTTCCAGCTT 
CATCGTTTCT TGGCCGACTA CCGTGACTTG ATTAGCTGGA TTGGAGACAT GAAAGCTGTC 
ATCGGAGCTG ATGAGCTTGC AAAAGACGTG GCTGGCGCTG AAGCTCTTCT TGAAAGTCAT 
CAAGAACACA AGGGAGAAAT TGATGCTCGT GCTGACTCGT TCAACCAAAC TGCATCCGCT 
GGACAAAAGC TCGTTGAGAT GGGAATTCCA GAGAGTCCAG AAGTTCAGGA GAAGCTCGAG 
AAACTTGACC ACGAGAAGTC TTCTCTTCTT GGATTGTGGG AGGAACGTCG TATTCTCTAC 
GAGCAATGTA TGGATCTCCA ACTCTTCTAT CGTGACACTG AACAAGCCGA AACCTGGATG 
AACAAGCAGG AAGCGTTCCT TGCCAACACT GATTTGGGAG ATTCACTAGA TTCTGTCGAG 
CACCTCATCA AGAAACACGA GGACTTTGAG AAATCGCTTG CTGCTCAAGA GGAGAAGATC 
AATGCACTTG ACGAGTTTGC TACCAAGCTC ATTCAAGGAC AGCATTACGC TGCTGATGAT 
GTTGCCGAGA GACGCCAAGC TCTTCTTGAC CGTCGTCGTC GTCTTCTAGA CAGAGCTCGT 
CAACGTGGAA ATGCTCTCAA GGAAAGCTAC AAGCGCCAGA CATTTGACCG TGACTGTGAT 
GAGATGGTTA GCTGGATCAC TGAAAAACTG TCCACCGCTC GTGACGATTC TTACTTGGAT 
CCTACCAACA TTCGTGGAAA ACTCCAAAAG CACATCAACT TTGAGCAAGA GCTTAAGGCT 
AACGAGAACC GTTTGGACGA TATTCGTTCT ACCGGAGAGC AAATCATCGA TAGTGGACAC 
TTTGCTGCTG ATCATATCGG AGACCGCCTT CGTCAAGTCA ACAACTTGTG GAACGAATTG 
GTCGATGCTA CAAACAAGAA GGGAGCCAAA CTTCGTGAAG CTGGAAACGA ACAGCAATTC 
AATCGCAACA TTGAAGATGT TGAAGCATGG TTGTCGGAGC TTGAAGGACA AGTTGCTTCC 
GAGGATTATG GAAAAGATCT TGTATCTGTT CAAAACCTTC AGAAGAAGAT TGGACTTATT 
GAGTCTGATT TTAACGCTCA CAACGATCGT GTTGATGGAA TCAAGCAACT TGCTCAACAG 
TTCCAAGAGG AGGAGCATTT CAATGCTCCA GTCATTGTTC GCAAGCAAGA GAATCTTCAG 
CAGCGCTACA ACGCTCTCCG TGATCCACTT GAGAAGAGAA AAAGAAAACT CGGAGAGTCC 
CACCAAGGAA ATCAACTGTT CCGCGATATT GAAGATGAGC TGGCTTGGAT CCGTGAGAAG 
GAACAAGTTG CTGGATCAAC CAATCGAGGA CGTGATTTGA TTGGAGTTCA AAATCTCATC 
AAGAAGCAGC AGGCTCTGAT CGCTGAGATT GCCAACCACG ATAGCCAAAT CGAGAGTGTC 
AGCAGTGCCG CCAATGACAT GATTCAACAA GGACACTTCT TGGCTCCAGA GATACGTGAT 
AAACTTGCTC AACTTCGTGA CAACTGGAGA ATTCTGAAGA GCAAGGCTGA GAAGAGACGC 
ACAGATCTTG ATGACTCACT CCAAGCCCAC CAATACCTTT CTGATGCCAA TGAGGCCGAT 
GCTTGGATGA GCGAGAAAGA ACCAATTGTT GGATCCACCG ACTATGGAAA GGACGAAGAT 
TCTGCCGAGG CTCTTCTCAA GAAGCATCGT GCTCTTCTTT CCGACTTGGA AGCTTTCAAG 
GGAACTATCG AGGATCTTCG CAAGCAAGCT TCTCAATGCA AGTACCAAGA GCAACCGATG 
GGACAACTCG GACGTGACTG TGTGTTGGCT CTTTACGATT ACCAAGAGAA ATCTCCTCGT 
GAGGTTTCCA TGAAGAAGGG AGATGTTCTC ACTCTTCTCA ATGCTAGCAA CAGAGACTGG 
TGGAAAGTTG AGGTCAATGA TCGTCAAGGA TTTGTTCCAG CTGCCTACGT TAAGAGAATC 
GAACCAGGAA CTGCTCAACA GCATGCTCAA CAACAGGTCA ACTCCATTGG AGGAAAGCAG 
TCGGAGATTG AGGACAAGTA CCAACGTCTC ATGATGCTCG GAGAGACTAG AAAGAGAAAG 
CTCGAGGAAG CTTGCAAAGG ATACCAACTT CTCCGTGAAG CCAACGACTT GGCTGAATGG 
ATCAAGTCTA GAACTAACAT GGCAGAAGAG GCTGTTGCTG CTCAACAAGA AATTGGAACT 
GACCTCGAGC AGGTTGAGGT TCTTCAAAAG AAGTTTGACG ACTTTAAAGG AGACCTCAAG 
GCTAACGAGG TTCGTCTCCA GGAGATGAAC CAAATTGCCA CCGCTCTCAC ATCGGTTGGA 
CAAACTGAAA CTGCTGTTCG TATTCGTCAG CAAATTGAGG ATCTCAACGC CAGATGGCGC 
GCATTGGAGG AGCAGACTGA GCAACGCGAA CAACAACTCG GATCTGCCCA CGAGGTGCAA 
CGCTTCCACC GTGATGTCGA CGAGACTCGT GATTGGATCC AGGAGAAGGA TGATGCCCTT 
GACAGTGAAG ATTTTGGACG TGACCTCCGT TCCGTTCAGG CTCTTCAACG CAAGCATGAA 
GGAGTTGAGC GTGATTTGGC TGCTCTTGGA GACAAGATTA AATCTCTTGA TGAGAAGGCC 
AACCGTCTTC GTCAATCTCA CCCAGAAGCT GCCGAGCAGA TTTACGATTT GCAACGTGAG 
CTCAATGAGC AATGGAACCG CCTCACGTCT AAGGCCAACA ACCGAAAAGA AAAGCTTCTT 
GATTCGTACG ACTACCAACG CTTTTTGTCT GACTACCGTG ATTTGATGCA ATGGATTGCT 
TCCATGAACC AACTTGTCAG CAGTCAAGAA CTTGCCAACG ATGTTACTGG AGCTGAGGCT 
CTTCTTGAGC GCCACCAGGA GTACCGCACC GAAATTGACT CCCGTGCTGC CACCTTCCAA 
GCTTTTGATC AATTTGGAAA CCAACTTCTT AACAGCCACC ACTACGCTGC AGGAGACATT 
GAAAACCGTC TTCAAGGTGT CAACGAGGCT CGTAAAGGAC TTGAAGACGC ATGGGTTGCT 
CGTCGTAACA TTCTCGATCA ATGCCTCGAA CTTCAACTCT TCTACCGTGA TTGTGAGCAA 
GCCGACACTT GGATGTCAGC TCGTGAAGCT TTCTTGGCTC AAGAGGATCC ATCTGGAGAC 
AATGTTGAGT CTTTGATCAA GAAACACGAG GACTTTGACA AAGCCATCAA CACTCAAGAG 
GAGAAAATCA AGGGACTCAA GCTCTTTGCT GAATCTCTCA TCAAGAACAA TCATTACGAT 
TCGCCAGCAG TCACTCGCAA GAGAGACCAG ATTCTCGATA GATGGAATGG TCTTAAGGAG 
GCTCTCATTC AGAAGAGATT CAAGCTTGGA GAGTCACAAA CTCTTCAACA GTTTAGTCGT 
GATGCGGATG AGATTGAGAA CTGGATGACT GAGAAGTTCC AGATTGCTCA AGAGGAGAAC 
TACCGCGATC CAACCAACAT TCAGCAGAAA CATCAGAAGC AACAAGCCTT CGAAGCCGAG 
CTTCATGCCA ATTCGGACAG AATCGCTGCT ATCATTCAAG CTGGAAACAA CCTCATTGAG 
AACGCCAAGT GCGGAGGAGG TGAAGCTGCT GTCAGCGCTC GACTGAAGGC TCTCAACGAC 
CAATGGGACC TTCTTGTCAA GACCACCACC GAGAAGAGTT ACCGTTTGAA AGAGGCTAAC 
AAGCAGAAGA GCTTCATGGC TGCTGTCAAG GATCTTGAGT TCTGGCTGGG AGAGGTTGAA 
ATTCTCCTCC AATCTGAAGA CTATGGAAAA GATTTGGCTT CCGTTGAAAA CCTGCTCAAA 
AAACATTCTC TTCTTGAGGC TGACATTGTT GCCCACCAGG ATCGTGTTGG AGAGATGAAC 
GAGCAAGCTG ATTCTCTTCT CGAGAACGAT CAATTCCAAG GACAACAAAT CGCTGAGAGA 
AGAAAGTTGA TTGCTGATCG TTATGATGGT GTCAAGAAGA TGGCTACCGA TCGTCGTGAT 
AAATTGAGCA AGGCCCTCAA TGTTCACCAG TTCTTCCGTG ATATTGATGA TGAGGAGTCT 
TGGATTAAGG AGAAGAAGCT TCTCGTTTCT TCTGATGACT ATGGACGTGA TCTTCCAGGA 
GTTCAAAACC TTCGCCGCAA GCATCGTCGT ATTGATACCG AGCTCGCTTC CCATGAGCCT 
CAAGTTTCTC TTGTCAAGCT GAAGGGAGAA GAGCTGTTGA GATCTGCAGC CGAAGCGGGA 
GTTGGAGAGG ATCAAATTAA GAAGCGTATG GAGGATCTTG AGCAAAGCTG GGGACAAATC 
CGTGATCTTA CTGGAAACCG TCATCAACGC CTCGACGAGT CTGAAGCCTT CCAAGCTTTC 
TTGGGTGATG TTGAAGAGGA AGAGGCATGG ATGAATGAGA AACAACAAAT CCTGGGATCC 
GACAACTTTG GAGAAAACAT GGCTGGAGTG CAAGGACTTC TCAAGAAGCA CGATACGTTC 
CAAGTCGATC TTGAGCTTCA CAAGCAACGT GTTGCTGATT TGATCAACAA GGGAGACACT 
CTTATTCACA ATGGAAACCA TCATGCTCCT CATATCAAGC AGAGATGCGA CCAACTCCGT 
GCTCGTCTTA TTGAGATTGA GAGCATGGCC GAAGGACGTC TTGCCAAGCT TCGTGATAAC 
TCTGCCTACC TTCAATTCAT GTGGAAGTGT GATGTTGTGG AATCGTGGAT TGCTGAGAAG 
GAGCAACAAG TTCGTTCCGA AGACTTTGGA AGAGATCTCT CATCTGTTCA AATCCTCCTC 
ACCAAGCAAG AAGCTTTCGA CGCTGGACTC AACGCTTTCG AACATGAAGG AATTCAAAGA 
ATCACCGAGC TCAAAGACCA ACTTGTTTCG AGTGAGCACC AACAATCTCC AGCCATTGAG 
AAACGTCATA CGAACGTCAT CCAACGTTGG CAGAACCTTC TTGCCCACTC CGAGGCTCGT 
CGTCAGAAGT TGTTGAAAAT GCAACAGCAA TTCAAGCAAA TCGAGGAGCT TTACCTTGCC 
TTTGCCAAGA AGGCTTCCAC TTTCAACTCA TGGTTCGAGA ACGCTGAGGA GGACTTGACT 
GACCCTGTCC GATGCAACTC TTTGGAAGAG ATCCGTGCCT TGCGCGACGC CCATGGTGAG 
TTCCAGCGCT CATTGTCCTC CGCTGAAGAG GACTTCCGCC AACTCCAAGA CCTTGATCGT 
CGGATCAAGT CGTTCAATGT GGGACCCAAC CCTTACACGT GGTTCACTAT GGATGCCCTC 
GAAGACACGT GGCGCAACCT GCAGAGAATC ATCAAGGAGC GCGAGCAGGA GCTTGCCAGA 
GAGCACCAAA GACAGGAGGA GAACGACAAA TTGCGACGCG AGTTTGCCAA GTTGGCCAAT 
GCTTTCCATG CTTGGTTGAC AAATACCAGA CAGGAGATGA TGGAAGCTGG AGGTACATTG 
GAAGAACAAC TCGATGCTGT TGAGAGGAAA GCAAAGGAAA TCAAGGCGAA CAAAGGTCAG 
CTGCGGCAGA TTGAAGAGAA GGGTGCTATG TTGGAGAGAA ACCTTATCCT AGACAATAGG 
TATACTGAAC ATTCGACAGT AGGAATTGCG CAAGCTTGGG ATCAATTGGA TCAGCTAGCC 
ATGCGAATGC AACACAATTT GGAACAACAG ATTCAAGCCA GAAATCAATC TGGAGTATCG 
GAAGAAGCCC TTCGTGAGTT CTCAATGATG TTCAAGCACT TCGACAAGGA GAAAACTGGA 
AGACTCGACC ACCAGCAGTT CAAGAGTTGT CTGCGAGCTC TTGGATACGA TCTGCCGATG 
GTGGACGAAG GCCAACCAGA ACCCGAGTTC CAGAGAATTT TGGATATTGT CGACCCGAAC 
AGAGATGGTT ACGTAACTCT GCAAGAGTAC ATGGCGTTCA TGATCAGCAA AGAAACCGAG 
AACATCCAGT CTTCGGAAGA AATCGAGATG GCATTCAGAG CTCTTTCGAA GGAATTCCGA 
CCATATGTCA CGGCAGAGGA GCTCTACGCG AACCTGACAC CGGAGCAAGC CGAATTCTGC 
ATACGAAGAA TGAAGCCATA CATGGACGCC ATTTCAGGTC GTTCCATCCA AGGTGGTCTC 
GACTACGAGC AGTTTGTGCA TGCCTTGTTC CAGAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001257000.1
CDS	1..7299
Translation	MADSNDTHAPPEPVLEVPPPQEIRILETADDIHQRRQEVLGHYSIFKDHSQTKRDRLEEARQFQYFKRDADELNVWILEKLQTAQEENFRDPSNLQAKIQKHEAFEAEVQAHAKTIANLDKTGNAMIQHNHFASEHIKKRLEELHALWDKLFFKLKDKGIKLQQALKLLHFIRQCDEILYWIREKETYVTAEDMGMDLEHVEILQRKFDDFLKELGNHQYRINEINQAADKLVEEGHSEHDQIYKKRDDVNDAWHRLNTLAATRKEGLFGAHQVQRFNRDADETLAWIGEKDLALSSDDYGRDLNNVQALQRKHEGTERDLAALEGKMLQLEKEAHKLAETHPDRAEAIHQKNDDTKAAWNALKEKAQHRKDGLERSFQLHRFLADYRDLISWIGDMKAVIGADELAKDVAGAEALLESHQEHKGEIDARADSFNQTASAGQKLVEMGIPESPEVQEKLEKLDHEKSSLLGLWEERRILYEQCMDLQLFYRDTEQAETWMNKQEAFLANTDLGDSLDSVEHLIKKHEDFEKSLAAQEEKINALDEFATKLIQGQHYAADDVAERRQALLDRRRRLLDRARQRGNALKESYKRQTFDRDCDEMVSWITEKLSTARDDSYLDPTNIRGKLQKHINFEQELKANENRLDDIRSTGEQIIDSGHFAADHIGDRLRQVNNLWNELVDATNKKGAKLREAGNEQQFNRNIEDVEAWLSELEGQVASEDYGKDLVSVQNLQKKIGLIESDFNAHNDRVDGIKQLAQQFQEEEHFNAPVIVRKQENLQQRYNALRDPLEKRKRKLGESHQGNQLFRDIEDELAWIREKEQVAGSTNRGRDLIGVQNLIKKQQALIAEIANHDSQIESVSSAANDMIQQGHFLAPEIRDKLAQLRDNWRILKSKAEKRRTDLDDSLQAHQYLSDANEADAWMSEKEPIVGSTDYGKDEDSAEALLKKHRALLSDLEAFKGTIEDLRKQASQCKYQEQPMGQLGRDCVLALYDYQEKSPREVSMKKGDVLTLLNASNRDWWKVEVNDRQGFVPAAYVKRIEPGTAQQHAQQQVNSIGGKQSEIEDKYQRLMMLGETRKRKLEEACKGYQLLREANDLAEWIKSRTNMAEEAVAAQQEIGTDLEQVEVLQKKFDDFKGDLKANEVRLQEMNQIATALTSVGQTETAVRIRQQIEDLNARWRALEEQTEQREQQLGSAHEVQRFHRDVDETRDWIQEKDDALDSEDFGRDLRSVQALQRKHEGVERDLAALGDKIKSLDEKANRLRQSHPEAAEQIYDLQRELNEQWNRLTSKANNRKEKLLDSYDYQRFLSDYRDLMQWIASMNQLVSSQELANDVTGAEALLERHQEYRTEIDSRAATFQAFDQFGNQLLNSHHYAAGDIENRLQGVNEARKGLEDAWVARRNILDQCLELQLFYRDCEQADTWMSAREAFLAQEDPSGDNVESLIKKHEDFDKAINTQEEKIKGLKLFAESLIKNNHYDSPAVTRKRDQILDRWNGLKEALIQKRFKLGESQTLQQFSRDADEIENWMTEKFQIAQEENYRDPTNIQQKHQKQQAFEAELHANSDRIAAIIQAGNNLIENAKCGGGEAAVSARLKALNDQWDLLVKTTTEKSYRLKEANKQKSFMAAVKDLEFWLGEVEILLQSEDYGKDLASVENLLKKHSLLEADIVAHQDRVGEMNEQADSLLENDQFQGQQIAERRKLIADRYDGVKKMATDRRDKLSKALNVHQFFRDIDDEESWIKEKKLLVSSDDYGRDLPGVQNLRRKHRRIDTELASHEPQVSLVKLKGEELLRSAAEAGVGEDQIKKRMEDLEQSWGQIRDLTGNRHQRLDESEAFQAFLGDVEEEEAWMNEKQQILGSDNFGENMAGVQGLLKKHDTFQVDLELHKQRVADLINKGDTLIHNGNHHAPHIKQRCDQLRARLIEIESMAEGRLAKLRDNSAYLQFMWKCDVVESWIAEKEQQVRSEDFGRDLSSVQILLTKQEAFDAGLNAFEHEGIQRITELKDQLVSSEHQQSPAIEKRHTNVIQRWQNLLAHSEARRQKLLKMQQQFKQIEELYLAFAKKASTFNSWFENAEEDLTDPVRCNSLEEIRALRDAHGEFQRSLSSAEEDFRQLQDLDRRIKSFNVGPNPYTWFTMDALEDTWRNLQRIIKEREQELAREHQRQEENDKLRREFAKLANAFHAWLTNTRQEMMEAGGTLEEQLDAVERKAKEIKANKGQLRQIEEKGAMLERNLILDNRYTEHSTVGIAQAWDQLDQLAMRMQHNLEQQIQARNQSGVSEEALREFSMMFKHFDKEKTGRLDHQQFKSCLRALGYDLPMVDEGQPEPEFQRILDIVDPNRDGYVTLQEYMAFMISKETENIQSSEEIEMAFRALSKEFRPYVTAEELYANLTPEQAEFCIRRMKPYMDAISGRSIQGGLDYEQFVHALFQS

Target ORF information:

RefSeq Version	NM_001270071.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans SPeCtrin (spc-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001270071.1

ORF Insert Sequence:

ATGGCTGATT CGAATGACAC TCATGCTCCA CCAGAGCCGG TGCTAGAAGT ACCGCCACCA 
CAGGAGATTC GTATTCTGGA GACTGCTGAT GACATTCACC AACGTCGTCA GGAAGTATTA 
GGTCACTATT CAATTTTCAA GGATCACTCG CAGACAAAAC GAGATCGTCT CGAGGAAGCT 
CGTCAGTTTC AATATTTCAA ACGAGATGCT GATGAGTTGA ACGTTTGGAT TCTTGAAAAG 
CTCCAGACAG CTCAGGAAGA AAACTTCAGA GATCCGAGCA ATCTCCAGGC CAAGATCCAG 
AAGCATGAAG CGTTCGAAGC TGAGGTTCAA GCTCACGCTA AGACCATCGC CAACCTCGAC 
AAGACCGGAA ACGCTATGAT CCAACACAAT CATTTCGCTA GCGAACACAT CAAGAAACGC 
TTGGAAGAGT TGCACGCCCT CTGGGATAAG CTCTTCTTCA AACTGAAGGA TAAGGGAATT 
AAGCTACAAC AAGCACTTAA ACTTTTGCAT TTTATTCGTC AATGCGATGA AATTCTCTAC 
TGGATTCGAG AGAAGGAAAC CTATGTCACC GCAGAAGACA TGGGAATGGA TCTCGAACAC 
GTTGAGATAC TTCAGCGTAA ATTTGACGAC TTTTTGAAAG AACTCGGTAA CCATCAGTAC 
CGAATCAATG AGATCAATCA AGCTGCTGAC AAACTTGTCG AGGAGGGACA CTCTGAGCAC 
GATCAGATCT ACAAGAAGCG CGATGACGTT AATGATGCTT GGCATCGTTT GAACACTTTG 
GCGGCCACTC GTAAGGAAGG GCTCTTTGGA GCTCATCAAG TTCAACGCTT CAATCGCGAT 
GCAGACGAGA CCCTTGCATG GATTGGAGAG AAGGATCTTG CTTTGTCAAG CGATGATTAC 
GGAAGAGACC TCAATAATGT TCAAGCTCTT CAACGCAAGC ATGAAGGAAC TGAACGTGAT 
CTTGCTGCTC TTGAAGGAAA GATGCTCCAA TTGGAGAAAG AAGCTCATAA GTTGGCAGAA 
ACTCATCCAG ATCGTGCTGA AGCGATTCAT CAAAAGAACG ATGACACCAA AGCCGCCTGG 
AATGCACTCA AGGAGAAGGC TCAACACCGC AAAGATGGAC TTGAGAGAAG CTTCCAGCTT 
CATCGTTTCT TGGCCGACTA CCGTGACTTG ATTAGCTGGA TTGGAGACAT GAAAGCTGTC 
ATCGGAGCTG ATGAGCTTGC AAAAGACGTG GCTGGCGCTG AAGCTCTTCT TGAAAGTCAT 
CAAGAACACA AGGGAGAAAT TGATGCTCGT GCTGACTCGT TCAACCAAAC TGCATCCGCT 
GGACAAAAGC TCGTTGAGAT GGGAATTCCA GAGAGTCCAG AAGTTCAGGA GAAGCTCGAG 
AAACTTGACC ACGAGAAGTC TTCTCTTCTT GGATTGTGGG AGGAACGTCG TATTCTCTAC 
GAGCAATGTA TGGATCTCCA ACTCTTCTAT CGTGACACTG AACAAGCCGA AACCTGGATG 
AACAAGCAGG AAGCGTTCCT TGCCAACACT GATTTGGGAG ATTCACTAGA TTCTGTCGAG 
CACCTCATCA AGAAACACGA GGACTTTGAG AAATCGCTTG CTGCTCAAGA GGAGAAGATC 
AATGCACTTG ACGAGTTTGC TACCAAGCTC ATTCAAGGAC AGCATTACGC TGCTGATGAT 
GTTGCCGAGA GACGCCAAGC TCTTCTTGAC CGTCGTCGTC GTCTTCTAGA CAGAGCTCGT 
CAACGTGGAA ATGCTCTCAA GGAAAGCTAC AAGCGCCAGA CATTTGACCG TGACTGTGAT 
GAGATGGTTA GCTGGATCAC TGAAAAACTG TCCACCGCTC GTGACGATTC TTACTTGGAT 
CCTACCAACA TTCGTGGAAA ACTCCAAAAG CACATCAACT TTGAGCAAGA GCTTAAGGCT 
AACGAGAACC GTTTGGACGA TATTCGTTCT ACCGGAGAGC AAATCATCGA TAGTGGACAC 
TTTGCTGCTG ATCATATCGG AGACCGCCTT CGTCAAGTCA ACAACTTGTG GAACGAATTG 
GTCGATGCTA CAAACAAGAA GGGAGCCAAA CTTCGTGAAG CTGGAAACGA ACAGCAATTC 
AATCGCAACA TTGAAGATGT TGAAGCATGG TTGTCGGAGC TTGAAGGACA AGTTGCTTCC 
GAGGATTATG GAAAAGATCT TGTATCTGTT CAAAACCTTC AGAAGAAGAT TGGACTTATT 
GAGTCTGATT TTAACGCTCA CAACGATCGT GTTGATGGAA TCAAGCAACT TGCTCAACAG 
TTCCAAGAGG AGGAGCATTT CAATGCTCCA GTCATTGTTC GCAAGCAAGA GAATCTTCAG 
CAGCGCTACA ACGCTCTCCG TGATCCACTT GAGAAGAGAA AAAGAAAACT CGGAGAGTCC 
CACCAAGGAA ATCAACTGTT CCGCGATATT GAAGATGAGC TGGCTTGGAT CCGTGAGAAG 
GAACAAGTTG CTGGATCAAC CAATCGAGGA CGTGATTTGA TTGGAGTTCA AAATCTCATC 
AAGAAGCAGC AGGCTCTGAT CGCTGAGATT GCCAACCACG ATAGCCAAAT CGAGAGTGTC 
AGCAGTGCCG CCAATGACAT GATTCAACAA GGACACTTCT TGGCTCCAGA GATACGTGAT 
AAACTTGCTC AACTTCGTGA CAACTGGAGA ATTCTGAAGA GCAAGGCTGA GAAGAGACGC 
ACAGATCTTG ATGACTCACT CCAAGCCCAC CAATACCTTT CTGATGCCAA TGAGGCCGAT 
GCTTGGATGA GCGAGAAAGA ACCAATTGTT GGATCCACCG ACTATGGAAA GGACGAAGAT 
TCTGCCGAGG CTCTTCTCAA GAAGCATCGT GCTCTTCTTT CCGACTTGGA AGCTTTCAAG 
GGAACTATCG AGGATCTTCG CAAGCAAGCT TCTCAATGCA AGTACCAAGA GCAACCGATG 
GGACAACTCG GACGTGACTG TGTGTTGGCT CTTTACGATT ACCAAGAGAA ATCTCCTCGT 
GAGGTTTCCA TGAAGAAGGG AGATGTTCTC ACTCTTCTCA ATGCTAGCAA CAGAGACTGG 
TGGAAAGTTG AGGTCAATGA TCGTCAAGGA TTTGTTCCAG CTGCCTACGT TAAGAGAATC 
GAACCAGGAA CTGCTCAACA GCATGCTCAA CAACAGGTCA ACTCCATTGG AGGAAAGCAG 
TCGGAGATTG AGGACAAGTA CCAACGTCTC ATGATGCTCG GAGAGACTAG AAAGAGAAAG 
CTCGAGGAAG CTTGCAAAGG ATACCAACTT CTCCGTGAAG CCAACGACTT GGCTGAATGG 
ATCAAGTCTA GAACTAACAT GGCAGAAGAG GCTGTTGCTG CTCAACAAGA AATTGGAACT 
GACCTCGAGC AGGTTGAGGT TCTTCAAAAG AAGTTTGACG ACTTTAAAGG AGACCTCAAG 
GCTAACGAGG TTCGTCTCCA GGAGATGAAC CAAATTGCCA CCGCTCTCAC ATCGGTTGGA 
CAAACTGAAA CTGCTGTTCG TATTCGTCAG CAAATTGAGG ATCTCAACGC CAGATGGCGC 
GCATTGGAGG AGCAGACTGA GCAACGCGAA CAACAACTCG GATCTGCCCA CGAGGTGCAA 
CGCTTCCACC GTGATGTCGA CGAGACTCGT GATTGGATCC AGGAGAAGGA TGATGCCCTT 
GACAGTGAAG ATTTTGGACG TGACCTCCGT TCCGTTCAGG CTCTTCAACG CAAGCATGAA 
GGAGTTGAGC GTGATTTGGC TGCTCTTGGA GACAAGATTA AATCTCTTGA TGAGAAGGCC 
AACCGTCTTC GTCAATCTCA CCCAGAAGCT GCCGAGCAGA TTTACGATTT GCAACGTGAG 
CTCAATGAGC AATGGAACCG CCTCACGTCT AAGGCCAACA ACCGAAAAGA AAAGCTTCTT 
GATTCGTACG ACTACCAACG CTTTTTGTCT GACTACCGTG ATTTGATGCA ATGGATTGCT 
TCCATGAACC AACTTGTCAG CAGTCAAGAA CTTGCCAACG ATGTTACTGG AGCTGAGGCT 
CTTCTTGAGC GCCACCAGGA GTACCGCACC GAAATTGACT CCCGTGCTGC CACCTTCCAA 
GCTTTTGATC AATTTGGAAA CCAACTTCTT AACAGCCACC ACTACGCTGC AGGAGACATT 
GAAAACCGTC TTCAAGGTGT CAACGAGGCT CGTAAAGGAC TTGAAGACGC ATGGGTTGCT 
CGTCGTAACA TTCTCGATCA ATGCCTCGAA CTTCAACTCT TCTACCGTGA TTGTGAGCAA 
GCCGACACTT GGATGTCAGC TCGTGAAGCT TTCTTGGCTC AAGAGGATCC ATCTGGAGAC 
AATGTTGAGT CTTTGATCAA GAAACACGAG GACTTTGACA AAGCCATCAA CACTCAAGAG 
GAGAAAATCA AGGGACTCAA GCTCTTTGCT GAATCTCTCA TCAAGAACAA TCATTACGAT 
TCGCCAGCAG TCACTCGCAA GAGAGACCAG ATTCTCGATA GATGGAATGG TCTTAAGGAG 
GCTCTCATTC AGAAGAGATT CAAGCTTGGA GAGTCACAAA CTCTTCAACA GTTTAGTCGT 
GATGCGGATG AGATTGAGAA CTGGATGACT GAGAAGTTCC AGATTGCTCA AGAGGAGAAC 
TACCGCGATC CAACCAACAT TCAGCAGAAA CATCAGAAGC AACAAGCCTT CGAAGCCGAG 
CTTCATGCCA ATTCGGACAG AATCGCTGCT ATCATTCAAG CTGGAAACAA CCTCATTGAG 
AACGCCAAGT GCGGAGGAGG TGAAGCTGCT GTCAGCGCTC GACTGAAGGC TCTCAACGAC 
CAATGGGACC TTCTTGTCAA GACCACCACC GAGAAGAGTT ACCGTTTGAA AGAGGCTAAC 
AAGCAGAAGA GCTTCATGGC TGCTGTCAAG GATCTTGAGT TCTGGCTGGG AGAGGTTGAA 
ATTCTCCTCC AATCTGAAGA CTATGGAAAA GATTTGGCTT CCGTTGAAAA CCTGCTCAAA 
AAACATTCTC TTCTTGAGGC TGACATTGTT GCCCACCAGG ATCGTGTTGG AGAGATGAAC 
GAGCAAGCTG ATTCTCTTCT CGAGAACGAT CAATTCCAAG GACAACAAAT CGCTGAGAGA 
AGAAAGTTGA TTGCTGATCG TTATGATGGT GTCAAGAAGA TGGCTACCGA TCGTCGTGAT 
AAATTGAGCA AGGCCCTCAA TGTTCACCAG TTCTTCCGTG ATATTGATGA TGAGGAGTCT 
TGGATTAAGG AGAAGAAGCT TCTCGTTTCT TCTGATGACT ATGGACGTGA TCTTCCAGGA 
GTTCAAAACC TTCGCCGCAA GCATCGTCGT ATTGATACCG AGCTCGCTTC CCATGAGCCT 
CAAGTTTCTC TTGTCAAGCT GAAGGGAGAA GAGCTGTTGA GATCTGCAGC CGAAGCGGGA 
GTTGGAGAGG ATCAAATTAA GAAGCGTATG GAGGATCTTG AGCAAAGCTG GGGACAAATC 
CGTGATCTTA CTGGAAACCG TCATCAACGC CTCGACGAGT CTGAAGCCTT CCAAGCTTTC 
TTGGGTGATG TTGAAGAGGA AGAGGCATGG ATGAATGAGA AACAACAAAT CCTGGGATCC 
GACAACTTTG GAGAAAACAT GGCTGGAGTG CAAGGACTTC TCAAGAAGCA CGATACGTTC 
CAAGTCGATC TTGAGCTTCA CAAGCAACGT GTTGCTGATT TGATCAACAA GGGAGACACT 
CTTATTCACA ATGGAAACCA TCATGCTCCT CATATCAAGC AGAGATGCGA CCAACTCCGT 
GCTCGTCTTA TTGAGATTGA GAGCATGGCC GAAGGACGTC TTGCCAAGCT TCGTGATAAC 
TCTGCCTACC TTCAATTCAT GTGGAAGTGT GATGTTGTGG AATCGTGGAT TGCTGAGAAG 
GAGCAACAAG TTCGTTCCGA AGACTTTGGA AGAGATCTCT CATCTGTTCA AATCCTCCTC 
ACCAAGCAAG AAGCTTTCGA CGCTGGACTC AACGCTTTCG AACATGAAGG AATTCAAAGA 
ATCACCGAGC TCAAAGACCA ACTTGTTTCG AGTGAGCACC AACAATCTCC AGCCATTGAG 
AAACGTCATA CGAACGTCAT CCAACGTTGG CAGAACCTTC TTGCCCACTC CGAGGCTCGT 
CGTCAGAAGT TGTTGAAAAT GCAACAGCAA TTCAAGCAAA TCGAGGAGCT TTACCTTGCC 
TTTGCCAAGA AGGCTTCCAC TTTCAACTCA TGGTTCGAGA ACGCTGAGGA GGACTTGACT 
GACCCTGTCC GATGCAACTC TTTGGAAGAG ATCCGTGCCT TGCGCGACGC CCATGGTGAG 
TTCCAGCGCT CATTGTCCTC CGCTGAAGAG GACTTCCGCC AACTCCAAGA CCTTGATCGT 
CGGATCAAGT CGTTCAATGT GGGACCCAAC CCTTACACGT GGTTCACTAT GGATGCCCTC 
GAAGACACGT GGCGCAACCT GCAGAGAATC ATCAAGGAGC GCGAGCAGGA GCTTGCCAGA 
GAGCACCAAA GACAGGAGGA GAACGACAAA TTGCGACGCG AGTTTGCCAA GTTGGCCAAT 
GCTTTCCATG CTTGGTTGAC AAATACCAGA CAGGAGATGA TGGAAGCTGG AGGTACATTG 
GAAGAACAAC TCGATGCTGT TGAGAGGAAA GCAAAGGAAA TCAAGGCGAA CAAAGGTCAG 
CTGCGGCAGA TTGAAGAGAA GGGTGCTATG TTGGAGAGAA ACCTTATCCT AGACAATAGG 
TATACTGAAC ATTCGACAGT AGGAATTGCG CAAGCTTGGG ATCAATTGGA TCAGCTAGCC 
ATGCGAATGC AACACAATTT GGAACAACAG ATTCAAGCCA GAAATCAATC TGGAGTATCG 
GAAGAAGCCC TTCGTGAGTT CTCAATGATG TTCAAGCACT TCGACAAGGA GAAAACTGGA 
AGACTCGACC ACCAGCAGTT CAAGAGTTGT CTGCGAGCTC TTGGATACGA TCTGCCGATG 
GTGGACGAAG GCCAACCAGA ACCCGAGTTC CAGAGAATTT TGGATATTGT CGACCCGAAC 
AGAGATGGTT ACGTAACTCT GCAAGAGTAC ATGGCGTTCA TGATCAGCAA AGAAACCGAG 
AACATCCAGT CTTCGGAAGA AATCGAGATG GCATTCAGAG CTCTTTCGAA GGAATTCCGA 
CCATATGTCA CGGCAGAGGA GCTCTACGCG AACCTGACAC CGGAGCAAGC CGAATTCTGC 
ATACGAAGAA TGAAGCCATA CATGGACGCC ATTTCAGGTC GTTCCATCCA AGGTGGTCTC 
GACTACGAGC AGTTTGTGCA TGCCTTGTTC CAGAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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spc-1 cDNA ORF clone, Caenorhabditis elegans

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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