sptan1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following sptan1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sptan1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa29834 XM_031906805.1
Latest version!
Xenopus tropicalis spectrin, alpha, non-erythrocytic 1 (sptan1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OXa29835 XM_031906806.1
Latest version!
Xenopus tropicalis spectrin, alpha, non-erythrocytic 1 (sptan1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OXa29836 XM_031906807.1
Latest version!
Xenopus tropicalis spectrin, alpha, non-erythrocytic 1 (sptan1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OXa29837 XM_031906808.1
Latest version!
Xenopus tropicalis spectrin, alpha, non-erythrocytic 1 (sptan1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OXa63342 XM_031906802.1
Latest version!
Xenopus tropicalis spectrin, alpha, non-erythrocytic 1 (sptan1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OXa63343 XM_031906803.1
Latest version!
Xenopus tropicalis spectrin, alpha, non-erythrocytic 1 (sptan1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OXa63344 XM_031906804.1
Latest version!
Xenopus tropicalis spectrin, alpha, non-erythrocytic 1 (sptan1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OXa22512 NM_001097205.1
Latest version!
Xenopus tropicalis spectrin, alpha, non-erythrocytic 1 (sptan1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OXa29834 XM_012967792.2
Latest version!
Xenopus tropicalis spectrin, alpha, non-erythrocytic 1 (sptan1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OXa29834 XM_012967793.1
Latest version!
Xenopus tropicalis spectrin, alpha, non-erythrocytic 1 (sptan1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OXa29835 XM_012967794.2
Latest version!
Xenopus tropicalis spectrin, alpha, non-erythrocytic 1 (sptan1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OXa29836 XM_012967795.2
Latest version!
Xenopus tropicalis spectrin, alpha, non-erythrocytic 1 (sptan1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OXa29837 XM_012967796.2
Latest version!
Xenopus tropicalis spectrin, alpha, non-erythrocytic 1 (sptan1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa29834
    Clone ID Related Accession (Same CDS sequence) XM_012967792.2 , XM_031906805.1 , XM_012967793.1
    Accession Version XM_031906805.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7431bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGATTCAG GTGGCGTGAA AGTGCTGGAG ACAGCGGAGG ACATCCAGGA AAGGCGCCAG 
    CAAGTCCTAG ACCGATACCT GCGGTTTAAG GAACTCTCTG TACTGAGACG TCAGAAGTTG
    GAGGATTCTT ACCGCTTCCA GTTCTTCCAG CGAGATGCTG ATGAACTGGA GAAATGGATC
    CAGGAGAAAC TCCAGATCGC ATCTGATGAG AACTACAAAG ATCCAACCAA TCTGCAGGGC
    AAATTGCAGA AACACCAGGC TTTTGAGGCA GAAGTACAGG CCAATGCAGG GGCAATTGTC
    AAACTGGACG AAACTGGAAA CCTGATGATT TCAGAAGGAC ATTTTTCATC AGAGACCATC
    AGAACCCGCC TGGTAGAGTT GCATCGTCTG TGGGAGCTGC TGCTCTTAAA GATGCAAGAG
    AAAGGGGTTA AATTACAACA GGCGCAGAAG CTTGTTCAGT ATCTACGGGA ATGTGAAGAT
    GTCCTAGACT GGATTAATGA CAAGGAGGCT ATTGTAACAT CTGAGGAGCT TGGTCAGGAT
    TTGGAACATG TGGAGGTCTT ACAGAAGAAG TTTGAAGAGT TTCAGACTGA TCTGGCAGCT
    CATGAAGAAA GAGTGAATGA AGTCAACCAG TTTGCAAGTA AACTGGCCCA GGAGGAACAC
    CCGGAGCTGG AGCTAATAAA GACCAAGCAG GATGAAGTCA ATGCAAGTTG GCAGCGCCTC
    AAAGGGCTGG CACTTCAGAG ACAAGGGAAG TTATTTGGAG CTGCAGAAGT TCAGCGTTTT
    AACAGAGATG TTGATGAAAC CATTGGATGG ATAAGAGAGA AGGAGCAGCT GATGGCATCT
    GATGACTTTG GCCGAGACCT GGCTAGCGTT CAAGCTCTCC TACGTAAACA CGAGGGTCTG
    GAGAGAGACC TTGCAGCACT GGAGGAAAAG GTAAAGGCCT TGTGTGCAGA ATCAGACCGT
    CTGCAGCAAT CCCATCCCAT GAACGCCCCT CAAATTCAAG TGAAAGGAGA AGAACTGATA
    ACCAACTGGG AGCAAATACG CACATTGGCA GCAGAACGAC ATGCTCGACT CAATGACTCT
    TACAAGCTTC AGCGTTTCCT TGCAGATTTT AGAGATCTGA CTAGCTGGGT GACTGAAATG
    AAAGCTCTGA TTAATGCCGA TGAGCTTGCC AACGATGTGG CTGGCGCAGA GGCTCTGCTT
    GACCGACACC AGGAACATAA GGGGGAAATT GATGCTCATG AAGATAGCTT TAAAGCTGCA
    GATGCTGCAG GTCAGGCCCT TCTGAACGGT GGCCACTACG CCTCAGATGA AGTCCGAGAA
    AAGCTTACTA TCTTAGCAGA AGAAAGGGCA GCTCTTCTAG AGCTTTGGGA GCTCCGTCGT
    CAGCAGTATG AACAGTGCAT GGACCTGCAG CTTTTTTACA GAGACACCGA ACAGGTGGAC
    AACTGGATGA GCAAACAGGA AGCTTTCCTG CTTAACGAGG ACTTGGGTGA TTCTCTAGAC
    AGTGTGGAAG CTCTTCTGAA AAAACATGAG GACTTTGAGA AGTCTCTTAG CGCCCAGGAA
    GAGAAAATCA CAGCTCTGGA TGAGTTTGCT ACAAAACTAA TTCAAAATAA CCACTATGCC
    AAGGAAGATG TGGCTACCCG CAGAGATGCT CTGCTGAGCC GCCGCAATGC ACTTCATGAA
    CGTGCACTCT ACCGCCGTAC TCAACTGGCA GACTCCTTTC ACCTGCAGCA GTTCTTTAGG
    GACTCTGACG AGCTGAAAAG TTGGATTAAT GAAAAGATGA AGACTGCAAC GGATGAGGCA
    TACAAGGATC CCTCCAACCT CCAGGGTAAG GTGCAGAAAC ACCAAGCTTT TGAGGCTGAA
    CTCTCCGCTA ACCAAAGCCG TATAGATGCC CTGGAGAATT CTGGGCAGAA ATTGATTGAT
    GTCAACCATT ATGCATCAGA TGAGGTGGCA GCACGTATGA GTGAGGTCAT CACTTTGTGG
    AAGAAGCTGC TGGAGGCCAC TGAATTGAAG GGGATCAAGC TGCGCGAAGC CAACCAGCAG
    CAGCAGTTCA ACCGTAATGT GGAGGATATA GAGCTGTGGC TGTATGAGGT TGAGGGGCAT
    TTGGCTTCAG ATGACTATGG AAAAGACCTG ACCAATGTTC AAAACTTGCA GAAAAAACAC
    GCCTTGCTGG AGGCTGATGT TGCAGCTCAC CAGGACCGTA TCGATGGCAT CACTATCCAA
    GCCCGTCAGT TCCAAGAAGC TGGTCATTTT GATGCTGACA ATATCAGGAA GAAACAGGAG
    GCCCTTGTTA ATAGATATGA AGCTTTGAAA GAACCCATGG TTGCACGCAA GCAGAAGCTG
    TCAGACTCTC TGCGTCTTCA ACAGCTTTTC AGGGATGTTG AAGATGAGGA AACCTGGATA
    AGGGAGAAGG AACCCATTGC AGCGTCCACC AACAGAGGCA AGGATTTGAT TGGAGTCCAG
    AATCTCCTGA AGAAACACCA GGCTTTGCAG GCTGAAATTG CAGGGCACGA ACCACGAATT
    AAAGCTGTTA CCCAGAAAGG AGAATCTATG ATCTCTGAAG GCCACTTTGC TTCTGATGAA
    GTGAAGGGCA AATTAAGGGA GCTGAATGAT AAGTGGGCCA CCCTGAAGAA CAAAGCATCT
    CAGCGCCGAC AGGACCTTGA AGATTCTCTG CAAGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCAGAGT CCTGGATGAG AGAGAAGGAA CCTATTGTTG GAAGCACCGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC CCTTCTGAAG AAACATGAAG CGCTTATGTC AGATCTGCGA
    GCATACGGAA GCAGTATTCA GGGACTTAGA GAGCAGGCCC AGGCCTGCAG GCAACAAGTT
    GCTCCAACTG ATGATGAAAC TGGAAAGGAG CTGGTGCTGG CATTGTATGA TTATCAAGAG
    AAGAGCCCTA GGGAGGTGAC AATGAAGAAA GGAGACATCT TGACCTTGCT AAACAGCACA
    AATAAGGATT GGTGGAAGGT GGAAGTGAAT GATCGTCAGG GCTTTGTACC TGCTGCGTAT
    GTGAAAAAGC TGGACCCAGC ACAGTCCGCA TCCCGGGAAA ACTTGCTGGA GGAACAGGGA
    AGCATCGCTC TCCGTCAGGA GCAGATCGAT GGCCAGGCTC ACATGTCTAA GGAAGCTGGC
    AGTGTTTCTC TGCGTATGAA TCAGGTTGAA GAATTGTATC ATAATATTTT GGAGATGGGA
    GAGAAGCGCA AAGATATGTT AGAAAAGAGC TGCAAGAAGT TTATGCTGTT CCGTGAGGCA
    AATGAGCTGC AGCAGTGGAT TAACGAGAAA GAAGCATCAC TCACTAACGA AGAGGTCGGG
    GCTGACCTGG AACAAGTGGA AGTGCTGCAG AAGAAGTTTG ATGATTTCCA GAAGGATCTG
    AAAGCAAATG AGTCACGCTT GAAGGACATC AACAAGGTGG CCGACGAGCT TGAATCTGAA
    GGCCTCATTA CAGAGGAAGT CCAGCCGGTG CAGCATCAGG AGGTGTATGG GATGCAGCGG
    GATGAAGCAG ACTCCAAATC GGCATCTCCA TGGAAGTCTG TTCGCACAGC TGTGCTCGCG
    GTGGCCACCT TCAACTCTAT CAAGGACTTG AACGAGCGCT GGAAATCACT TCAACAGCTG
    GCAGAAGAAA GGAGTCAGCT TCTTGGCAGT GCCCATGAAG TACAGAGATT CCACAGAGAT
    GCTGATGAGA CAAAAGAATG GATCGAAGAG AAGAACCAGG CTTTGAATAC AGACAGCTAT
    GGACATGACT TGGCCAGTGT GCAGGCTCTG CAACGAAAAC ATGAAGGCTT TGAGAGAGAC
    CTCGCCGCTC TGGGAGATAA GGTGAACTCT TTGGGAGAAA CTGCTGAGCG CCTGATCCAG
    TCACACCCTG AGTCTGCAGA TGACATTCAG GAAAAATGCA CAGAGCTGAA TCAGGCCTGG
    AACAGCCTTG GCACCCGGGC AAACCAGCGC AAGGAGAAAC TAGGCGATTC TCATGATCTG
    CAACGCTTCC TAAGTGACTT CAGAGATCTC ATGTCCTGGA TCAATGGAAT TCGGGGCTTG
    GTGTCATCAG ATGAACTTGC TAGAGATGTG ACTGGTGCAG AGGCTTTGCT GGAACGACAC
    CAGGAGCACA GGACTGAGAT TGATGCCAGA GCAGGCACTT TTCAGGCCTT TGAGCAGTTT
    GGGCAGCAAC TTCTGGCTCG TGGTCATTAT GATAGTCCTG AAATCAAGGA GAAGCTGGAT
    ATCTTGGAAC AGGAACGGGC AAGTTTGGAA AAGGCTTGGG TGGAACGCAG AATGATGTTG
    GATCAGTGCT TGGAGCTGCA GCTCTTCAAC AGAGACTGCG AGCAGGCAGA GAACTGGATG
    GCAGCCCGTG AAGCCTTCCT GAACAGTGAG GATAAAGGGG ATTCCCTGGA CAGTGTGGAG
    GCCCTTATCA AGAAACATGA AGACTTTGAT AAAGCCATTA ATGTCCAGGA AGAGAAGATT
    GCTGCCCTCC AGGCCTTCGC CGAACAATTG ATAATTGCTG ACCACTATGC CAAAGGAGAT
    ATTGCCTCCC GTCGCAATGA AGTCCTGGAC AGGTGGCGCC GTCTCAAGGC TCAGATGATT
    GAGAAGAGAT CCAAACTTGG GGAATCTCAG ACTCTGCAGC AGTTCAGCAG GGACGTCGAT
    GAGATCGAAG CCTGGATCAG CGAGAAATTG CAGACCGCCA GTGACGAGTC CTACAAAGAC
    CCCACAAACA TCCAGCTTTC CAAATTGCTG AGCAAACACC AGAAGCACCA GGCTTTCGAA
    GCTGAGCTCC ATGCCAACGC TGACCGTATT CGTGGAGTAG TAGATATGGG GAACTCCCTT
    ATCGAACGAG GTGCCTGTGC TGGCAGCGAG GATGCGGTGA AGGCTCGCCT AAGCGCACTC
    GCTGATCAAT GGCAGTTCCT TGTCAGCAAA TCCGCAGAGA AGAGCCAGAA ACTGAAGGAG
    GCAAACAAGC AGCAGAACTT TAACACTGGC ATCAAAGACT TTGATTTCTG GTTAACAGAG
    GTGGAGGCTT TGCTGGCATC TGAAGACTAT GGGAAGGACT TGGCATCTGT TAACAACCTG
    CTGAAGAAAC ACCAGCTTCT GGAAGCTGAC ATCTCTGCTC ATGAGGACCG CCTTAAAGAT
    CTTAATGGGC AGGCTGATAG CCTGATGACC AGCAGCGCCT TTGACACCAC CCAAGTAAAG
    GAGAAAAGGG ACGCCATCAA CGACCGCTTC CAGCGTATCA AAAGCATGGC CACCGCACGC
    CGCAGCAGGC TGAATGAGTC CCACCGTCTG CACCAGTTCT TCAGAGATCT TGATGATGAG
    GAATCATGGA TCAAGGAAAA GAAACTTTTG GTCGGATCTG ATGACTATGG ACGTGACTTG
    ACTGGAGTGC AAAATCTGAG GAAGAAACAC AAGAGATTAG AAGCTGAACT GGCAGCTCAT
    GAGCCAGCCA TTCAGGGTGT GCTGGACACT GGGAAGAAGC TTGCAGATGA CAACACTATT
    GGGAAAGATG AGATTCAGCA GCGACTGGCA CAGTTTCTAG AGCACTGGAA GGAGCTGAAG
    GAACAAGCAG CGGCTCGTGG TCAGAGACTG GAGGAATCTC TTGAGTACCA ACAATTTGTA
    GCCAATGTGG AGGAAGAGGA AGCCTGGATT AATGAGAAGA TGACTCTAGT GGCCAGTGAA
    GACTATGGTG ACACACTGGC TGCCATTCAG GGATTGCTGA AAAAGCACGA GGCATTTGAG
    ACAGACTTTA CAGTGCACAA AGACAGAGTA CATGATGTAT GTGCCAACGG GGAAGACCTG
    ATACAAAAGA ACAATCACCA TGTGGAGAAT ATTAGCGCCA AAATGAAGGG TCTCCGAGGG
    AAGGTGGCAG AACTAGAGAA GGCGGCTGCA CAGCGCAAGG CCAAACTTGA TGAGAATTCA
    GCTTTCCTGC AGTTCAACTG GAAGGCGGAT GTTGTGGAGT CCTGGATCGG TGAAAAAGAG
    AACAGCCTGA AGACTGATGA TTATGGCAGA GACCTGTCCT CTGTGCAGAC CCTTCTCACT
    AAGCAGGAAA CATTTGATGC TGGGCTGCAA GCTTTCCAGC AAGAGGGCAT TACCAACATC
    ACTGCACTGA AAGACCAGCT TCTGGCTGCA AAGCATGTGC AGTCCCGGGC CATAGAGTCT
    CGTCACGCTT CTCTGATGAA GCGGTGGAAC CAGTTGTTGG ATAACTCTGC TGCCCGCAAA
    AAGAAGTTAT TGGAGGCCCA GGAACACTAC AGAAAGGTTG AGGACTTGTT CCTAACATTT
    GCCAAGAAAG CCTCTGCCTT CAACAGCTGG TTTGAGAATG CCGAGGAGGA TTTGACTGAT
    CCTGTTCGCT GCAACTCCCT GGAGGAAATC AAGGCGCTTC GCGAGGCACA TGACGCCTTC
    CGCAATTCCC TCAGCTCCGC ACAGACAGAT TTCAACCAGC TGGCCGAACT GGACAGGCAA
    ATAAAGGGCT ACCGGGTAGC CTCCAATCCC TACACGTGGT TCACGATGGA GGCTTTGGAG
    GAGACATGGC GCAACCTGCA GAAAATCATT AAGGAACGAG AACTTGAGCT ACAAAAAGAA
    CAACGTAGGC AGGAAGAGAA CGATAAGCTC CGGCAAGAGT TTGCTCAGCA CGCCAATGCT
    TTCCACCAGT GGATTCAGGA GACAAGGACC TACCTTCTGG ACGGGTCCTG TATGGTGGAA
    GAATCTGGAA CACTGGAGTC ACAACTTGAA GCTACAAAGA GAAAGCATCA GGAGATCCGG
    GCCATGAGGA GTCAACTGAA GAAGATAGAA GATCTTGGCG CAGCCATGGA AGAGGCCCTG
    ATCTTGGATA ATAAATACAC AGAACACAGC ACTGTTGGGC TGGCACAGCA ATGGGACCAA
    CTGGATCAGC TAGGCATGAG AATGCAACAC AACCTAGAGC AACAGATCCA GGCCAGAAAC
    ACAACTGGGG TCACAGAGGA AGCACTCAAA GAATTCAGCA TGATGTTCAA GCATTTCGAC
    AAAGACAAGT CTGGCCGCCT CAACCACCAA GAATTCAAAT CTTGTCTGCG GTCCCTTGGC
    TATGATCTGC CCATGGTAGA AGAAGGAGAA CCAGACCCAG AGTTTGAAGC CATTTTGGAC
    ACTGTAGATC CCAACAGAGA TGGGCACGTT TCTCTACAGG AGTACATGGC TTTCATGATT
    AGCAGAGAAA CTGAAAACGT CAAGTCCAGT GAGGAGATAG AGAGCGCCTT CCGAGCACTG
    AGTACAGACC AGAAACCTTA TGTCACAAAG GAAGAACTAT ACCAGAACCT GACACGCGAA
    CAGGCAGATT ACTGCATCTC GCATATGAAA CCCTACATGG ACAGCAAGGG CCGCGAACTG
    CCCTCTGCAT ACGACTACGT GGAATTCACG CGCTCGCTAT TTGTGAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031762665.1
    CDS4..7434
    Translation

    Target ORF information:

    RefSeq Version XM_031906805.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis spectrin, alpha, non-erythrocytic 1 (sptan1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031906805.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGATTCAG GTGGCGTGAA AGTGCTGGAG ACAGCGGAGG ACATCCAGGA AAGGCGCCAG 
    CAAGTCCTAG ACCGATACCT GCGGTTTAAG GAACTCTCTG TACTGAGACG TCAGAAGTTG
    GAGGATTCTT ACCGCTTCCA GTTCTTCCAG CGAGATGCTG ATGAACTGGA GAAATGGATC
    CAGGAGAAAC TCCAGATCGC ATCTGATGAG AACTACAAAG ATCCAACCAA TCTGCAGGGC
    AAATTGCAGA AACACCAGGC TTTTGAGGCA GAAGTACAGG CCAATGCAGG GGCAATTGTC
    AAACTGGACG AAACTGGAAA CCTGATGATT TCAGAAGGAC ATTTTTCATC AGAGACCATC
    AGAACCCGCC TGGTAGAGTT GCATCGTCTG TGGGAGCTGC TGCTCTTAAA GATGCAAGAG
    AAAGGGGTTA AATTACAACA GGCGCAGAAG CTTGTTCAGT ATCTACGGGA ATGTGAAGAT
    GTCCTAGACT GGATTAATGA CAAGGAGGCT ATTGTAACAT CTGAGGAGCT TGGTCAGGAT
    TTGGAACATG TGGAGGTCTT ACAGAAGAAG TTTGAAGAGT TTCAGACTGA TCTGGCAGCT
    CATGAAGAAA GAGTGAATGA AGTCAACCAG TTTGCAAGTA AACTGGCCCA GGAGGAACAC
    CCGGAGCTGG AGCTAATAAA GACCAAGCAG GATGAAGTCA ATGCAAGTTG GCAGCGCCTC
    AAAGGGCTGG CACTTCAGAG ACAAGGGAAG TTATTTGGAG CTGCAGAAGT TCAGCGTTTT
    AACAGAGATG TTGATGAAAC CATTGGATGG ATAAGAGAGA AGGAGCAGCT GATGGCATCT
    GATGACTTTG GCCGAGACCT GGCTAGCGTT CAAGCTCTCC TACGTAAACA CGAGGGTCTG
    GAGAGAGACC TTGCAGCACT GGAGGAAAAG GTAAAGGCCT TGTGTGCAGA ATCAGACCGT
    CTGCAGCAAT CCCATCCCAT GAACGCCCCT CAAATTCAAG TGAAAGGAGA AGAACTGATA
    ACCAACTGGG AGCAAATACG CACATTGGCA GCAGAACGAC ATGCTCGACT CAATGACTCT
    TACAAGCTTC AGCGTTTCCT TGCAGATTTT AGAGATCTGA CTAGCTGGGT GACTGAAATG
    AAAGCTCTGA TTAATGCCGA TGAGCTTGCC AACGATGTGG CTGGCGCAGA GGCTCTGCTT
    GACCGACACC AGGAACATAA GGGGGAAATT GATGCTCATG AAGATAGCTT TAAAGCTGCA
    GATGCTGCAG GTCAGGCCCT TCTGAACGGT GGCCACTACG CCTCAGATGA AGTCCGAGAA
    AAGCTTACTA TCTTAGCAGA AGAAAGGGCA GCTCTTCTAG AGCTTTGGGA GCTCCGTCGT
    CAGCAGTATG AACAGTGCAT GGACCTGCAG CTTTTTTACA GAGACACCGA ACAGGTGGAC
    AACTGGATGA GCAAACAGGA AGCTTTCCTG CTTAACGAGG ACTTGGGTGA TTCTCTAGAC
    AGTGTGGAAG CTCTTCTGAA AAAACATGAG GACTTTGAGA AGTCTCTTAG CGCCCAGGAA
    GAGAAAATCA CAGCTCTGGA TGAGTTTGCT ACAAAACTAA TTCAAAATAA CCACTATGCC
    AAGGAAGATG TGGCTACCCG CAGAGATGCT CTGCTGAGCC GCCGCAATGC ACTTCATGAA
    CGTGCACTCT ACCGCCGTAC TCAACTGGCA GACTCCTTTC ACCTGCAGCA GTTCTTTAGG
    GACTCTGACG AGCTGAAAAG TTGGATTAAT GAAAAGATGA AGACTGCAAC GGATGAGGCA
    TACAAGGATC CCTCCAACCT CCAGGGTAAG GTGCAGAAAC ACCAAGCTTT TGAGGCTGAA
    CTCTCCGCTA ACCAAAGCCG TATAGATGCC CTGGAGAATT CTGGGCAGAA ATTGATTGAT
    GTCAACCATT ATGCATCAGA TGAGGTGGCA GCACGTATGA GTGAGGTCAT CACTTTGTGG
    AAGAAGCTGC TGGAGGCCAC TGAATTGAAG GGGATCAAGC TGCGCGAAGC CAACCAGCAG
    CAGCAGTTCA ACCGTAATGT GGAGGATATA GAGCTGTGGC TGTATGAGGT TGAGGGGCAT
    TTGGCTTCAG ATGACTATGG AAAAGACCTG ACCAATGTTC AAAACTTGCA GAAAAAACAC
    GCCTTGCTGG AGGCTGATGT TGCAGCTCAC CAGGACCGTA TCGATGGCAT CACTATCCAA
    GCCCGTCAGT TCCAAGAAGC TGGTCATTTT GATGCTGACA ATATCAGGAA GAAACAGGAG
    GCCCTTGTTA ATAGATATGA AGCTTTGAAA GAACCCATGG TTGCACGCAA GCAGAAGCTG
    TCAGACTCTC TGCGTCTTCA ACAGCTTTTC AGGGATGTTG AAGATGAGGA AACCTGGATA
    AGGGAGAAGG AACCCATTGC AGCGTCCACC AACAGAGGCA AGGATTTGAT TGGAGTCCAG
    AATCTCCTGA AGAAACACCA GGCTTTGCAG GCTGAAATTG CAGGGCACGA ACCACGAATT
    AAAGCTGTTA CCCAGAAAGG AGAATCTATG ATCTCTGAAG GCCACTTTGC TTCTGATGAA
    GTGAAGGGCA AATTAAGGGA GCTGAATGAT AAGTGGGCCA CCCTGAAGAA CAAAGCATCT
    CAGCGCCGAC AGGACCTTGA AGATTCTCTG CAAGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCAGAGT CCTGGATGAG AGAGAAGGAA CCTATTGTTG GAAGCACCGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC CCTTCTGAAG AAACATGAAG CGCTTATGTC AGATCTGCGA
    GCATACGGAA GCAGTATTCA GGGACTTAGA GAGCAGGCCC AGGCCTGCAG GCAACAAGTT
    GCTCCAACTG ATGATGAAAC TGGAAAGGAG CTGGTGCTGG CATTGTATGA TTATCAAGAG
    AAGAGCCCTA GGGAGGTGAC AATGAAGAAA GGAGACATCT TGACCTTGCT AAACAGCACA
    AATAAGGATT GGTGGAAGGT GGAAGTGAAT GATCGTCAGG GCTTTGTACC TGCTGCGTAT
    GTGAAAAAGC TGGACCCAGC ACAGTCCGCA TCCCGGGAAA ACTTGCTGGA GGAACAGGGA
    AGCATCGCTC TCCGTCAGGA GCAGATCGAT GGCCAGGCTC ACATGTCTAA GGAAGCTGGC
    AGTGTTTCTC TGCGTATGAA TCAGGTTGAA GAATTGTATC ATAATATTTT GGAGATGGGA
    GAGAAGCGCA AAGATATGTT AGAAAAGAGC TGCAAGAAGT TTATGCTGTT CCGTGAGGCA
    AATGAGCTGC AGCAGTGGAT TAACGAGAAA GAAGCATCAC TCACTAACGA AGAGGTCGGG
    GCTGACCTGG AACAAGTGGA AGTGCTGCAG AAGAAGTTTG ATGATTTCCA GAAGGATCTG
    AAAGCAAATG AGTCACGCTT GAAGGACATC AACAAGGTGG CCGACGAGCT TGAATCTGAA
    GGCCTCATTA CAGAGGAAGT CCAGCCGGTG CAGCATCAGG AGGTGTATGG GATGCAGCGG
    GATGAAGCAG ACTCCAAATC GGCATCTCCA TGGAAGTCTG TTCGCACAGC TGTGCTCGCG
    GTGGCCACCT TCAACTCTAT CAAGGACTTG AACGAGCGCT GGAAATCACT TCAACAGCTG
    GCAGAAGAAA GGAGTCAGCT TCTTGGCAGT GCCCATGAAG TACAGAGATT CCACAGAGAT
    GCTGATGAGA CAAAAGAATG GATCGAAGAG AAGAACCAGG CTTTGAATAC AGACAGCTAT
    GGACATGACT TGGCCAGTGT GCAGGCTCTG CAACGAAAAC ATGAAGGCTT TGAGAGAGAC
    CTCGCCGCTC TGGGAGATAA GGTGAACTCT TTGGGAGAAA CTGCTGAGCG CCTGATCCAG
    TCACACCCTG AGTCTGCAGA TGACATTCAG GAAAAATGCA CAGAGCTGAA TCAGGCCTGG
    AACAGCCTTG GCACCCGGGC AAACCAGCGC AAGGAGAAAC TAGGCGATTC TCATGATCTG
    CAACGCTTCC TAAGTGACTT CAGAGATCTC ATGTCCTGGA TCAATGGAAT TCGGGGCTTG
    GTGTCATCAG ATGAACTTGC TAGAGATGTG ACTGGTGCAG AGGCTTTGCT GGAACGACAC
    CAGGAGCACA GGACTGAGAT TGATGCCAGA GCAGGCACTT TTCAGGCCTT TGAGCAGTTT
    GGGCAGCAAC TTCTGGCTCG TGGTCATTAT GATAGTCCTG AAATCAAGGA GAAGCTGGAT
    ATCTTGGAAC AGGAACGGGC AAGTTTGGAA AAGGCTTGGG TGGAACGCAG AATGATGTTG
    GATCAGTGCT TGGAGCTGCA GCTCTTCAAC AGAGACTGCG AGCAGGCAGA GAACTGGATG
    GCAGCCCGTG AAGCCTTCCT GAACAGTGAG GATAAAGGGG ATTCCCTGGA CAGTGTGGAG
    GCCCTTATCA AGAAACATGA AGACTTTGAT AAAGCCATTA ATGTCCAGGA AGAGAAGATT
    GCTGCCCTCC AGGCCTTCGC CGAACAATTG ATAATTGCTG ACCACTATGC CAAAGGAGAT
    ATTGCCTCCC GTCGCAATGA AGTCCTGGAC AGGTGGCGCC GTCTCAAGGC TCAGATGATT
    GAGAAGAGAT CCAAACTTGG GGAATCTCAG ACTCTGCAGC AGTTCAGCAG GGACGTCGAT
    GAGATCGAAG CCTGGATCAG CGAGAAATTG CAGACCGCCA GTGACGAGTC CTACAAAGAC
    CCCACAAACA TCCAGCTTTC CAAATTGCTG AGCAAACACC AGAAGCACCA GGCTTTCGAA
    GCTGAGCTCC ATGCCAACGC TGACCGTATT CGTGGAGTAG TAGATATGGG GAACTCCCTT
    ATCGAACGAG GTGCCTGTGC TGGCAGCGAG GATGCGGTGA AGGCTCGCCT AAGCGCACTC
    GCTGATCAAT GGCAGTTCCT TGTCAGCAAA TCCGCAGAGA AGAGCCAGAA ACTGAAGGAG
    GCAAACAAGC AGCAGAACTT TAACACTGGC ATCAAAGACT TTGATTTCTG GTTAACAGAG
    GTGGAGGCTT TGCTGGCATC TGAAGACTAT GGGAAGGACT TGGCATCTGT TAACAACCTG
    CTGAAGAAAC ACCAGCTTCT GGAAGCTGAC ATCTCTGCTC ATGAGGACCG CCTTAAAGAT
    CTTAATGGGC AGGCTGATAG CCTGATGACC AGCAGCGCCT TTGACACCAC CCAAGTAAAG
    GAGAAAAGGG ACGCCATCAA CGACCGCTTC CAGCGTATCA AAAGCATGGC CACCGCACGC
    CGCAGCAGGC TGAATGAGTC CCACCGTCTG CACCAGTTCT TCAGAGATCT TGATGATGAG
    GAATCATGGA TCAAGGAAAA GAAACTTTTG GTCGGATCTG ATGACTATGG ACGTGACTTG
    ACTGGAGTGC AAAATCTGAG GAAGAAACAC AAGAGATTAG AAGCTGAACT GGCAGCTCAT
    GAGCCAGCCA TTCAGGGTGT GCTGGACACT GGGAAGAAGC TTGCAGATGA CAACACTATT
    GGGAAAGATG AGATTCAGCA GCGACTGGCA CAGTTTCTAG AGCACTGGAA GGAGCTGAAG
    GAACAAGCAG CGGCTCGTGG TCAGAGACTG GAGGAATCTC TTGAGTACCA ACAATTTGTA
    GCCAATGTGG AGGAAGAGGA AGCCTGGATT AATGAGAAGA TGACTCTAGT GGCCAGTGAA
    GACTATGGTG ACACACTGGC TGCCATTCAG GGATTGCTGA AAAAGCACGA GGCATTTGAG
    ACAGACTTTA CAGTGCACAA AGACAGAGTA CATGATGTAT GTGCCAACGG GGAAGACCTG
    ATACAAAAGA ACAATCACCA TGTGGAGAAT ATTAGCGCCA AAATGAAGGG TCTCCGAGGG
    AAGGTGGCAG AACTAGAGAA GGCGGCTGCA CAGCGCAAGG CCAAACTTGA TGAGAATTCA
    GCTTTCCTGC AGTTCAACTG GAAGGCGGAT GTTGTGGAGT CCTGGATCGG TGAAAAAGAG
    AACAGCCTGA AGACTGATGA TTATGGCAGA GACCTGTCCT CTGTGCAGAC CCTTCTCACT
    AAGCAGGAAA CATTTGATGC TGGGCTGCAA GCTTTCCAGC AAGAGGGCAT TACCAACATC
    ACTGCACTGA AAGACCAGCT TCTGGCTGCA AAGCATGTGC AGTCCCGGGC CATAGAGTCT
    CGTCACGCTT CTCTGATGAA GCGGTGGAAC CAGTTGTTGG ATAACTCTGC TGCCCGCAAA
    AAGAAGTTAT TGGAGGCCCA GGAACACTAC AGAAAGGTTG AGGACTTGTT CCTAACATTT
    GCCAAGAAAG CCTCTGCCTT CAACAGCTGG TTTGAGAATG CCGAGGAGGA TTTGACTGAT
    CCTGTTCGCT GCAACTCCCT GGAGGAAATC AAGGCGCTTC GCGAGGCACA TGACGCCTTC
    CGCAATTCCC TCAGCTCCGC ACAGACAGAT TTCAACCAGC TGGCCGAACT GGACAGGCAA
    ATAAAGGGCT ACCGGGTAGC CTCCAATCCC TACACGTGGT TCACGATGGA GGCTTTGGAG
    GAGACATGGC GCAACCTGCA GAAAATCATT AAGGAACGAG AACTTGAGCT ACAAAAAGAA
    CAACGTAGGC AGGAAGAGAA CGATAAGCTC CGGCAAGAGT TTGCTCAGCA CGCCAATGCT
    TTCCACCAGT GGATTCAGGA GACAAGGACC TACCTTCTGG ACGGGTCCTG TATGGTGGAA
    GAATCTGGAA CACTGGAGTC ACAACTTGAA GCTACAAAGA GAAAGCATCA GGAGATCCGG
    GCCATGAGGA GTCAACTGAA GAAGATAGAA GATCTTGGCG CAGCCATGGA AGAGGCCCTG
    ATCTTGGATA ATAAATACAC AGAACACAGC ACTGTTGGGC TGGCACAGCA ATGGGACCAA
    CTGGATCAGC TAGGCATGAG AATGCAACAC AACCTAGAGC AACAGATCCA GGCCAGAAAC
    ACAACTGGGG TCACAGAGGA AGCACTCAAA GAATTCAGCA TGATGTTCAA GCATTTCGAC
    AAAGACAAGT CTGGCCGCCT CAACCACCAA GAATTCAAAT CTTGTCTGCG GTCCCTTGGC
    TATGATCTGC CCATGGTAGA AGAAGGAGAA CCAGACCCAG AGTTTGAAGC CATTTTGGAC
    ACTGTAGATC CCAACAGAGA TGGGCACGTT TCTCTACAGG AGTACATGGC TTTCATGATT
    AGCAGAGAAA CTGAAAACGT CAAGTCCAGT GAGGAGATAG AGAGCGCCTT CCGAGCACTG
    AGTACAGACC AGAAACCTTA TGTCACAAAG GAAGAACTAT ACCAGAACCT GACACGCGAA
    CAGGCAGATT ACTGCATCTC GCATATGAAA CCCTACATGG ACAGCAAGGG CCGCGAACTG
    CCCTCTGCAT ACGACTACGT GGAATTCACG CGCTCGCTAT TTGTGAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29835
    Clone ID Related Accession (Same CDS sequence) XM_031906806.1 , XM_012967794.2
    Accession Version XM_031906806.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7413bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGATTCAG GTGGCGTGAA AGTGCTGGAG ACAGCGGAGG ACATCCAGGA AAGGCGCCAG 
    CAAGTCCTAG ACCGATACCT GCGGTTTAAG GAACTCTCTG TACTGAGACG TCAGAAGTTG
    GAGGATTCTT ACCGCTTCCA GTTCTTCCAG CGAGATGCTG ATGAACTGGA GAAATGGATC
    CAGGAGAAAC TCCAGATCGC ATCTGATGAG AACTACAAAG ATCCAACCAA TCTGCAGGGC
    AAATTGCAGA AACACCAGGC TTTTGAGGCA GAAGTACAGG CCAATGCAGG GGCAATTGTC
    AAACTGGACG AAACTGGAAA CCTGATGATT TCAGAAGGAC ATTTTTCATC AGAGACCATC
    AGAACCCGCC TGGTAGAGTT GCATCGTCTG TGGGAGCTGC TGCTCTTAAA GATGCAAGAG
    AAAGGGGTTA AATTACAACA GGCGCAGAAG CTTGTTCAGT ATCTACGGGA ATGTGAAGAT
    GTCCTAGACT GGATTAATGA CAAGGAGGCT ATTGTAACAT CTGAGGAGCT TGGTCAGGAT
    TTGGAACATG TGGAGGTCTT ACAGAAGAAG TTTGAAGAGT TTCAGACTGA TCTGGCAGCT
    CATGAAGAAA GAGTGAATGA AGTCAACCAG TTTGCAAGTA AACTGGCCCA GGAGGAACAC
    CCGGAGCTGG AGCTAATAAA GACCAAGCAG GATGAAGTCA ATGCAAGTTG GCAGCGCCTC
    AAAGGGCTGG CACTTCAGAG ACAAGGGAAG TTATTTGGAG CTGCAGAAGT TCAGCGTTTT
    AACAGAGATG TTGATGAAAC CATTGGATGG ATAAGAGAGA AGGAGCAGCT GATGGCATCT
    GATGACTTTG GCCGAGACCT GGCTAGCGTT CAAGCTCTCC TACGTAAACA CGAGGGTCTG
    GAGAGAGACC TTGCAGCACT GGAGGAAAAG GTAAAGGCCT TGTGTGCAGA ATCAGACCGT
    CTGCAGCAAT CCCATCCCAT GAACGCCCCT CAAATTCAAG TGAAAGGAGA AGAACTGATA
    ACCAACTGGG AGCAAATACG CACATTGGCA GCAGAACGAC ATGCTCGACT CAATGACTCT
    TACAAGCTTC AGCGTTTCCT TGCAGATTTT AGAGATCTGA CTAGCTGGGT GACTGAAATG
    AAAGCTCTGA TTAATGCCGA TGAGCTTGCC AACGATGTGG CTGGCGCAGA GGCTCTGCTT
    GACCGACACC AGGAACATAA GGGGGAAATT GATGCTCATG AAGATAGCTT TAAAGCTGCA
    GATGCTGCAG GTCAGGCCCT TCTGAACGGT GGCCACTACG CCTCAGATGA AGTCCGAGAA
    AAGCTTACTA TCTTAGCAGA AGAAAGGGCA GCTCTTCTAG AGCTTTGGGA GCTCCGTCGT
    CAGCAGTATG AACAGTGCAT GGACCTGCAG CTTTTTTACA GAGACACCGA ACAGGTGGAC
    AACTGGATGA GCAAACAGGA AGCTTTCCTG CTTAACGAGG ACTTGGGTGA TTCTCTAGAC
    AGTGTGGAAG CTCTTCTGAA AAAACATGAG GACTTTGAGA AGTCTCTTAG CGCCCAGGAA
    GAGAAAATCA CAGCTCTGGA TGAGTTTGCT ACAAAACTAA TTCAAAATAA CCACTATGCC
    AAGGAAGATG TGGCTACCCG CAGAGATGCT CTGCTGAGCC GCCGCAATGC ACTTCATGAA
    CGTGCACTCT ACCGCCGTAC TCAACTGGCA GACTCCTTTC ACCTGCAGCA GTTCTTTAGG
    GACTCTGACG AGCTGAAAAG TTGGATTAAT GAAAAGATGA AGACTGCAAC GGATGAGGCA
    TACAAGGATC CCTCCAACCT CCAGGGTAAG GTGCAGAAAC ACCAAGCTTT TGAGGCTGAA
    CTCTCCGCTA ACCAAAGCCG TATAGATGCC CTGGAGAATT CTGGGCAGAA ATTGATTGAT
    GTCAACCATT ATGCATCAGA TGAGGTGGCA GCACGTATGA GTGAGGTCAT CACTTTGTGG
    AAGAAGCTGC TGGAGGCCAC TGAATTGAAG GGGATCAAGC TGCGCGAAGC CAACCAGCAG
    CAGCAGTTCA ACCGTAATGT GGAGGATATA GAGCTGTGGC TGTATGAGGT TGAGGGGCAT
    TTGGCTTCAG ATGACTATGG AAAAGACCTG ACCAATGTTC AAAACTTGCA GAAAAAACAC
    GCCTTGCTGG AGGCTGATGT TGCAGCTCAC CAGGACCGTA TCGATGGCAT CACTATCCAA
    GCCCGTCAGT TCCAAGAAGC TGGTCATTTT GATGCTGACA ATATCAGGAA GAAACAGGAG
    GCCCTTGTTA ATAGATATGA AGCTTTGAAA GAACCCATGG TTGCACGCAA GCAGAAGCTG
    TCAGACTCTC TGCGTCTTCA ACAGCTTTTC AGGGATGTTG AAGATGAGGA AACCTGGATA
    AGGGAGAAGG AACCCATTGC AGCGTCCACC AACAGAGGCA AGGATTTGAT TGGAGTCCAG
    AATCTCCTGA AGAAACACCA GGCTTTGCAG GCTGAAATTG CAGGGCACGA ACCACGAATT
    AAAGCTGTTA CCCAGAAAGG AGAATCTATG ATCTCTGAAG GCCACTTTGC TTCTGATGAA
    GTGAAGGGCA AATTAAGGGA GCTGAATGAT AAGTGGGCCA CCCTGAAGAA CAAAGCATCT
    CAGCGCCGAC AGGACCTTGA AGATTCTCTG CAAGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCAGAGT CCTGGATGAG AGAGAAGGAA CCTATTGTTG GAAGCACCGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC CCTTCTGAAG AAACATGAAG CGCTTATGTC AGATCTGCGA
    GCATACGGAA GCAGTATTCA GGGACTTAGA GAGCAGGCCC AGGCCTGCAG GCAACAAGTT
    GCTCCAACTG ATGATGAAAC TGGAAAGGAG CTGGTGCTGG CATTGTATGA TTATCAAGAG
    AAGAGCCCTA GGGAGGTGAC AATGAAGAAA GGAGACATCT TGACCTTGCT AAACAGCACA
    AATAAGGATT GGTGGAAGGT GGAAGTGAAT GATCGTCAGG GCTTTGTACC TGCTGCGTAT
    GTGAAAAAGC TGGACCCAGC ACAGTCCGCA TCCCGGGAAA ACTTGCTGGA GGAACAGGGA
    AGCATCGCTC TCCGTCAGGA GCAGATCGAT GGCCAGGCTC ACATGTCTAA GGAAGCTGGC
    AGTGTTTCTC TGCGTATGAA TCAGGTTGAA GAATTGTATC ATAATATTTT GGAGATGGGA
    GAGAAGCGCA AAGATATGTT AGAAAAGAGC TGCAAGAAGT TTATGCTGTT CCGTGAGGCA
    AATGAGCTGC AGCAGTGGAT TAACGAGAAA GAAGCATCAC TCACTAACGA AGAGGTCGGG
    GCTGACCTGG AACAAGTGGA AGTGCTGCAG AAGAAGTTTG ATGATTTCCA GAAGGATCTG
    AAAGCAAATG AGTCACGCTT GAAGGACATC AACAAGGTGG CCGACGAGCT TGAATCTGAA
    GGCCTCATTA CAGAGGAAGT CCAGCCGGTG CAGCATCAGG AGGTGTATGG GATGCAGCGG
    GATGAAGCAG ACTCCAAATC GGCATCTCCA TGGAAGTCTG TTCGCACAGC TGTGCTCGCG
    GTGGCCACCT TCAACTCTAT CAAGGACTTG AACGAGCGCT GGAAATCACT TCAACAGCTG
    GCAGAAGAAA GGAGTCAGCT TCTTGGCAGT GCCCATGAAG TACAGAGATT CCACAGAGAT
    GCTGATGAGA CAAAAGAATG GATCGAAGAG AAGAACCAGG CTTTGAATAC AGACAGCTAT
    GGACATGACT TGGCCAGTGT GCAGGCTCTG CAACGAAAAC ATGAAGGCTT TGAGAGAGAC
    CTCGCCGCTC TGGGAGATAA GGTGAACTCT TTGGGAGAAA CTGCTGAGCG CCTGATCCAG
    TCACACCCTG AGTCTGCAGA TGACATTCAG GAAAAATGCA CAGAGCTGAA TCAGGCCTGG
    AACAGCCTTG GCACCCGGGC AAACCAGCGC AAGGAGAAAC TAGGCGATTC TCATGATCTG
    CAACGCTTCC TAAGTGACTT CAGAGATCTC ATGTCCTGGA TCAATGGAAT TCGGGGCTTG
    GTGTCATCAG ATGAACTTGC TAGAGATGTG ACTGGTGCAG AGGCTTTGCT GGAACGACAC
    CAGGAGCACA GGACTGAGAT TGATGCCAGA GCAGGCACTT TTCAGGCCTT TGAGCAGTTT
    GGGCAGCAAC TTCTGGCTCG TGGTCATTAT GATAGTCCTG AAATCAAGGA GAAGCTGGAT
    ATCTTGGAAC AGGAACGGGC AAGTTTGGAA AAGGCTTGGG TGGAACGCAG AATGATGTTG
    GATCAGTGCT TGGAGCTGCA GCTCTTCAAC AGAGACTGCG AGCAGGCAGA GAACTGGATG
    GCAGCCCGTG AAGCCTTCCT GAACAGTGAG GATAAAGGGG ATTCCCTGGA CAGTGTGGAG
    GCCCTTATCA AGAAACATGA AGACTTTGAT AAAGCCATTA ATGTCCAGGA AGAGAAGATT
    GCTGCCCTCC AGGCCTTCGC CGAACAATTG ATAATTGCTG ACCACTATGC CAAAGGAGAT
    ATTGCCTCCC GTCGCAATGA AGTCCTGGAC AGGTGGCGCC GTCTCAAGGC TCAGATGATT
    GAGAAGAGAT CCAAACTTGG GGAATCTCAG ACTCTGCAGC AGTTCAGCAG GGACGTCGAT
    GAGATCGAAG CCTGGATCAG CGAGAAATTG CAGACCGCCA GTGACGAGTC CTACAAAGAC
    CCCACAAACA TCCAGCTTTC CAAATTGCTG AGCAAACACC AGAAGCACCA GGCTTTCGAA
    GCTGAGCTCC ATGCCAACGC TGACCGTATT CGTGGAGTAG TAGATATGGG GAACTCCCTT
    ATCGAACGAG GTGCCTGTGC TGGCAGCGAG GATGCGGTGA AGGCTCGCCT AAGCGCACTC
    GCTGATCAAT GGCAGTTCCT TGTCAGCAAA TCCGCAGAGA AGAGCCAGAA ACTGAAGGAG
    GCAAACAAGC AGCAGAACTT TAACACTGGC ATCAAAGACT TTGATTTCTG GTTAACAGAG
    GTGGAGGCTT TGCTGGCATC TGAAGACTAT GGGAAGGACT TGGCATCTGT TAACAACCTG
    CTGAAGAAAC ACCAGCTTCT GGAAGCTGAC ATCTCTGCTC ATGAGGACCG CCTTAAAGAT
    CTTAATGGGC AGGCTGATAG CCTGATGACC AGCAGCGCCT TTGACACCAC CCAAGTAAAG
    GAGAAAAGGG ACGCCATCAA CGACCGCTTC CAGCGTATCA AAAGCATGGC CACCGCACGC
    CGCAGCAGGC TGAATGAGTC CCACCGTCTG CACCAGTTCT TCAGAGATCT TGATGATGAG
    GAATCATGGA TCAAGGAAAA GAAACTTTTG GTCGGATCTG ATGACTATGG ACGTGACTTG
    ACTGGAGTGC AAAATCTGAG GAAGAAACAC AAGAGATTAG AAGCTGAACT GGCAGCTCAT
    GAGCCAGCCA TTCAGGGTGT GCTGGACACT GGGAAGAAGC TTGCAGATGA CAACACTATT
    GGGAAAGATG AGATTCAGCA GCGACTGGCA CAGTTTCTAG AGCACTGGAA GGAGCTGAAG
    GAACAAGCAG CGGCTCGTGG TCAGAGACTG GAGGAATCTC TTGAGTACCA ACAATTTGTA
    GCCAATGTGG AGGAAGAGGA AGCCTGGATT AATGAGAAGA TGACTCTAGT GGCCAGTGAA
    GACTATGGTG ACACACTGGC TGCCATTCAG GGATTGCTGA AAAAGCACGA GGCATTTGAG
    ACAGACTTTA CAGTGCACAA AGACAGAGTA CATGATGTAT GTGCCAACGG GGAAGACCTG
    ATACAAAAGA ACAATCACCA TGTGGAGAAT ATTAGCGCCA AAATGAAGGG TCTCCGAGGG
    AAGGTGGCAG AACTAGAGAA GGCGGCTGCA CAGCGCAAGG CCAAACTTGA TGAGAATTCA
    GCTTTCCTGC AGTTCAACTG GAAGGCGGAT GTTGTGGAGT CCTGGATCGG TGAAAAAGAG
    AACAGCCTGA AGACTGATGA TTATGGCAGA GACCTGTCCT CTGTGCAGAC CCTTCTCACT
    AAGCAGGAAA CATTTGATGC TGGGCTGCAA GCTTTCCAGC AAGAGGGCAT TACCAACATC
    ACTGCACTGA AAGACCAGCT TCTGGCTGCA AAGCATGTGC AGTCCCGGGC CATAGAGTCT
    CGTCACGCTT CTCTGATGAA GCGGTGGAAC CAGTTGTTGG ATAACTCTGC TGCCCGCAAA
    AAGAAGTTAT TGGAGGCCCA GGAACACTAC AGAAAGGTTG AGGACTTGTT CCTAACATTT
    GCCAAGAAAG CCTCTGCCTT CAACAGCTGG TTTGAGAATG CCGAGGAGGA TTTGACTGAT
    CCTGTTCGCT GCAACTCCCT GGAGGAAATC AAGGCGCTTC GCGAGGCACA TGACGCCTTC
    CGCAATTCCC TCAGCTCCGC ACAGACAGAT TTCAACCAGC TGGCCGAACT GGACAGGCAA
    ATAAAGGGCT ACCGGGTAGC CTCCAATCCC TACACGTGGT TCACGATGGA GGCTTTGGAG
    GAGACATGGC GCAACCTGCA GAAAATCATT AAGGAACGAG AACTTGAGCT ACAAAAAGAA
    CAACGTAGGC AGGAAGAGAA CGATAAGCTC CGGCAAGAGT TTGCTCAGCA CGCCAATGCT
    TTCCACCAGT GGATTCAGGA GACAAGGTCC TGTATGGTGG AAGAATCTGG AACACTGGAG
    TCACAACTTG AAGCTACAAA GAGAAAGCAT CAGGAGATCC GGGCCATGAG GAGTCAACTG
    AAGAAGATAG AAGATCTTGG CGCAGCCATG GAAGAGGCCC TGATCTTGGA TAATAAATAC
    ACAGAACACA GCACTGTTGG GCTGGCACAG CAATGGGACC AACTGGATCA GCTAGGCATG
    AGAATGCAAC ACAACCTAGA GCAACAGATC CAGGCCAGAA ACACAACTGG GGTCACAGAG
    GAAGCACTCA AAGAATTCAG CATGATGTTC AAGCATTTCG ACAAAGACAA GTCTGGCCGC
    CTCAACCACC AAGAATTCAA ATCTTGTCTG CGGTCCCTTG GCTATGATCT GCCCATGGTA
    GAAGAAGGAG AACCAGACCC AGAGTTTGAA GCCATTTTGG ACACTGTAGA TCCCAACAGA
    GATGGGCACG TTTCTCTACA GGAGTACATG GCTTTCATGA TTAGCAGAGA AACTGAAAAC
    GTCAAGTCCA GTGAGGAGAT AGAGAGCGCC TTCCGAGCAC TGAGTACAGA CCAGAAACCT
    TATGTCACAA AGGAAGAACT ATACCAGAAC CTGACACGCG AACAGGCAGA TTACTGCATC
    TCGCATATGA AACCCTACAT GGACAGCAAG GGCCGCGAAC TGCCCTCTGC ATACGACTAC
    GTGGAATTCA CGCGCTCGCT ATTTGTGAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031762666.1
    CDS4..7416
    Translation

    Target ORF information:

    RefSeq Version XM_031906806.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis spectrin, alpha, non-erythrocytic 1 (sptan1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031906806.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGATTCAG GTGGCGTGAA AGTGCTGGAG ACAGCGGAGG ACATCCAGGA AAGGCGCCAG 
    CAAGTCCTAG ACCGATACCT GCGGTTTAAG GAACTCTCTG TACTGAGACG TCAGAAGTTG
    GAGGATTCTT ACCGCTTCCA GTTCTTCCAG CGAGATGCTG ATGAACTGGA GAAATGGATC
    CAGGAGAAAC TCCAGATCGC ATCTGATGAG AACTACAAAG ATCCAACCAA TCTGCAGGGC
    AAATTGCAGA AACACCAGGC TTTTGAGGCA GAAGTACAGG CCAATGCAGG GGCAATTGTC
    AAACTGGACG AAACTGGAAA CCTGATGATT TCAGAAGGAC ATTTTTCATC AGAGACCATC
    AGAACCCGCC TGGTAGAGTT GCATCGTCTG TGGGAGCTGC TGCTCTTAAA GATGCAAGAG
    AAAGGGGTTA AATTACAACA GGCGCAGAAG CTTGTTCAGT ATCTACGGGA ATGTGAAGAT
    GTCCTAGACT GGATTAATGA CAAGGAGGCT ATTGTAACAT CTGAGGAGCT TGGTCAGGAT
    TTGGAACATG TGGAGGTCTT ACAGAAGAAG TTTGAAGAGT TTCAGACTGA TCTGGCAGCT
    CATGAAGAAA GAGTGAATGA AGTCAACCAG TTTGCAAGTA AACTGGCCCA GGAGGAACAC
    CCGGAGCTGG AGCTAATAAA GACCAAGCAG GATGAAGTCA ATGCAAGTTG GCAGCGCCTC
    AAAGGGCTGG CACTTCAGAG ACAAGGGAAG TTATTTGGAG CTGCAGAAGT TCAGCGTTTT
    AACAGAGATG TTGATGAAAC CATTGGATGG ATAAGAGAGA AGGAGCAGCT GATGGCATCT
    GATGACTTTG GCCGAGACCT GGCTAGCGTT CAAGCTCTCC TACGTAAACA CGAGGGTCTG
    GAGAGAGACC TTGCAGCACT GGAGGAAAAG GTAAAGGCCT TGTGTGCAGA ATCAGACCGT
    CTGCAGCAAT CCCATCCCAT GAACGCCCCT CAAATTCAAG TGAAAGGAGA AGAACTGATA
    ACCAACTGGG AGCAAATACG CACATTGGCA GCAGAACGAC ATGCTCGACT CAATGACTCT
    TACAAGCTTC AGCGTTTCCT TGCAGATTTT AGAGATCTGA CTAGCTGGGT GACTGAAATG
    AAAGCTCTGA TTAATGCCGA TGAGCTTGCC AACGATGTGG CTGGCGCAGA GGCTCTGCTT
    GACCGACACC AGGAACATAA GGGGGAAATT GATGCTCATG AAGATAGCTT TAAAGCTGCA
    GATGCTGCAG GTCAGGCCCT TCTGAACGGT GGCCACTACG CCTCAGATGA AGTCCGAGAA
    AAGCTTACTA TCTTAGCAGA AGAAAGGGCA GCTCTTCTAG AGCTTTGGGA GCTCCGTCGT
    CAGCAGTATG AACAGTGCAT GGACCTGCAG CTTTTTTACA GAGACACCGA ACAGGTGGAC
    AACTGGATGA GCAAACAGGA AGCTTTCCTG CTTAACGAGG ACTTGGGTGA TTCTCTAGAC
    AGTGTGGAAG CTCTTCTGAA AAAACATGAG GACTTTGAGA AGTCTCTTAG CGCCCAGGAA
    GAGAAAATCA CAGCTCTGGA TGAGTTTGCT ACAAAACTAA TTCAAAATAA CCACTATGCC
    AAGGAAGATG TGGCTACCCG CAGAGATGCT CTGCTGAGCC GCCGCAATGC ACTTCATGAA
    CGTGCACTCT ACCGCCGTAC TCAACTGGCA GACTCCTTTC ACCTGCAGCA GTTCTTTAGG
    GACTCTGACG AGCTGAAAAG TTGGATTAAT GAAAAGATGA AGACTGCAAC GGATGAGGCA
    TACAAGGATC CCTCCAACCT CCAGGGTAAG GTGCAGAAAC ACCAAGCTTT TGAGGCTGAA
    CTCTCCGCTA ACCAAAGCCG TATAGATGCC CTGGAGAATT CTGGGCAGAA ATTGATTGAT
    GTCAACCATT ATGCATCAGA TGAGGTGGCA GCACGTATGA GTGAGGTCAT CACTTTGTGG
    AAGAAGCTGC TGGAGGCCAC TGAATTGAAG GGGATCAAGC TGCGCGAAGC CAACCAGCAG
    CAGCAGTTCA ACCGTAATGT GGAGGATATA GAGCTGTGGC TGTATGAGGT TGAGGGGCAT
    TTGGCTTCAG ATGACTATGG AAAAGACCTG ACCAATGTTC AAAACTTGCA GAAAAAACAC
    GCCTTGCTGG AGGCTGATGT TGCAGCTCAC CAGGACCGTA TCGATGGCAT CACTATCCAA
    GCCCGTCAGT TCCAAGAAGC TGGTCATTTT GATGCTGACA ATATCAGGAA GAAACAGGAG
    GCCCTTGTTA ATAGATATGA AGCTTTGAAA GAACCCATGG TTGCACGCAA GCAGAAGCTG
    TCAGACTCTC TGCGTCTTCA ACAGCTTTTC AGGGATGTTG AAGATGAGGA AACCTGGATA
    AGGGAGAAGG AACCCATTGC AGCGTCCACC AACAGAGGCA AGGATTTGAT TGGAGTCCAG
    AATCTCCTGA AGAAACACCA GGCTTTGCAG GCTGAAATTG CAGGGCACGA ACCACGAATT
    AAAGCTGTTA CCCAGAAAGG AGAATCTATG ATCTCTGAAG GCCACTTTGC TTCTGATGAA
    GTGAAGGGCA AATTAAGGGA GCTGAATGAT AAGTGGGCCA CCCTGAAGAA CAAAGCATCT
    CAGCGCCGAC AGGACCTTGA AGATTCTCTG CAAGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCAGAGT CCTGGATGAG AGAGAAGGAA CCTATTGTTG GAAGCACCGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC CCTTCTGAAG AAACATGAAG CGCTTATGTC AGATCTGCGA
    GCATACGGAA GCAGTATTCA GGGACTTAGA GAGCAGGCCC AGGCCTGCAG GCAACAAGTT
    GCTCCAACTG ATGATGAAAC TGGAAAGGAG CTGGTGCTGG CATTGTATGA TTATCAAGAG
    AAGAGCCCTA GGGAGGTGAC AATGAAGAAA GGAGACATCT TGACCTTGCT AAACAGCACA
    AATAAGGATT GGTGGAAGGT GGAAGTGAAT GATCGTCAGG GCTTTGTACC TGCTGCGTAT
    GTGAAAAAGC TGGACCCAGC ACAGTCCGCA TCCCGGGAAA ACTTGCTGGA GGAACAGGGA
    AGCATCGCTC TCCGTCAGGA GCAGATCGAT GGCCAGGCTC ACATGTCTAA GGAAGCTGGC
    AGTGTTTCTC TGCGTATGAA TCAGGTTGAA GAATTGTATC ATAATATTTT GGAGATGGGA
    GAGAAGCGCA AAGATATGTT AGAAAAGAGC TGCAAGAAGT TTATGCTGTT CCGTGAGGCA
    AATGAGCTGC AGCAGTGGAT TAACGAGAAA GAAGCATCAC TCACTAACGA AGAGGTCGGG
    GCTGACCTGG AACAAGTGGA AGTGCTGCAG AAGAAGTTTG ATGATTTCCA GAAGGATCTG
    AAAGCAAATG AGTCACGCTT GAAGGACATC AACAAGGTGG CCGACGAGCT TGAATCTGAA
    GGCCTCATTA CAGAGGAAGT CCAGCCGGTG CAGCATCAGG AGGTGTATGG GATGCAGCGG
    GATGAAGCAG ACTCCAAATC GGCATCTCCA TGGAAGTCTG TTCGCACAGC TGTGCTCGCG
    GTGGCCACCT TCAACTCTAT CAAGGACTTG AACGAGCGCT GGAAATCACT TCAACAGCTG
    GCAGAAGAAA GGAGTCAGCT TCTTGGCAGT GCCCATGAAG TACAGAGATT CCACAGAGAT
    GCTGATGAGA CAAAAGAATG GATCGAAGAG AAGAACCAGG CTTTGAATAC AGACAGCTAT
    GGACATGACT TGGCCAGTGT GCAGGCTCTG CAACGAAAAC ATGAAGGCTT TGAGAGAGAC
    CTCGCCGCTC TGGGAGATAA GGTGAACTCT TTGGGAGAAA CTGCTGAGCG CCTGATCCAG
    TCACACCCTG AGTCTGCAGA TGACATTCAG GAAAAATGCA CAGAGCTGAA TCAGGCCTGG
    AACAGCCTTG GCACCCGGGC AAACCAGCGC AAGGAGAAAC TAGGCGATTC TCATGATCTG
    CAACGCTTCC TAAGTGACTT CAGAGATCTC ATGTCCTGGA TCAATGGAAT TCGGGGCTTG
    GTGTCATCAG ATGAACTTGC TAGAGATGTG ACTGGTGCAG AGGCTTTGCT GGAACGACAC
    CAGGAGCACA GGACTGAGAT TGATGCCAGA GCAGGCACTT TTCAGGCCTT TGAGCAGTTT
    GGGCAGCAAC TTCTGGCTCG TGGTCATTAT GATAGTCCTG AAATCAAGGA GAAGCTGGAT
    ATCTTGGAAC AGGAACGGGC AAGTTTGGAA AAGGCTTGGG TGGAACGCAG AATGATGTTG
    GATCAGTGCT TGGAGCTGCA GCTCTTCAAC AGAGACTGCG AGCAGGCAGA GAACTGGATG
    GCAGCCCGTG AAGCCTTCCT GAACAGTGAG GATAAAGGGG ATTCCCTGGA CAGTGTGGAG
    GCCCTTATCA AGAAACATGA AGACTTTGAT AAAGCCATTA ATGTCCAGGA AGAGAAGATT
    GCTGCCCTCC AGGCCTTCGC CGAACAATTG ATAATTGCTG ACCACTATGC CAAAGGAGAT
    ATTGCCTCCC GTCGCAATGA AGTCCTGGAC AGGTGGCGCC GTCTCAAGGC TCAGATGATT
    GAGAAGAGAT CCAAACTTGG GGAATCTCAG ACTCTGCAGC AGTTCAGCAG GGACGTCGAT
    GAGATCGAAG CCTGGATCAG CGAGAAATTG CAGACCGCCA GTGACGAGTC CTACAAAGAC
    CCCACAAACA TCCAGCTTTC CAAATTGCTG AGCAAACACC AGAAGCACCA GGCTTTCGAA
    GCTGAGCTCC ATGCCAACGC TGACCGTATT CGTGGAGTAG TAGATATGGG GAACTCCCTT
    ATCGAACGAG GTGCCTGTGC TGGCAGCGAG GATGCGGTGA AGGCTCGCCT AAGCGCACTC
    GCTGATCAAT GGCAGTTCCT TGTCAGCAAA TCCGCAGAGA AGAGCCAGAA ACTGAAGGAG
    GCAAACAAGC AGCAGAACTT TAACACTGGC ATCAAAGACT TTGATTTCTG GTTAACAGAG
    GTGGAGGCTT TGCTGGCATC TGAAGACTAT GGGAAGGACT TGGCATCTGT TAACAACCTG
    CTGAAGAAAC ACCAGCTTCT GGAAGCTGAC ATCTCTGCTC ATGAGGACCG CCTTAAAGAT
    CTTAATGGGC AGGCTGATAG CCTGATGACC AGCAGCGCCT TTGACACCAC CCAAGTAAAG
    GAGAAAAGGG ACGCCATCAA CGACCGCTTC CAGCGTATCA AAAGCATGGC CACCGCACGC
    CGCAGCAGGC TGAATGAGTC CCACCGTCTG CACCAGTTCT TCAGAGATCT TGATGATGAG
    GAATCATGGA TCAAGGAAAA GAAACTTTTG GTCGGATCTG ATGACTATGG ACGTGACTTG
    ACTGGAGTGC AAAATCTGAG GAAGAAACAC AAGAGATTAG AAGCTGAACT GGCAGCTCAT
    GAGCCAGCCA TTCAGGGTGT GCTGGACACT GGGAAGAAGC TTGCAGATGA CAACACTATT
    GGGAAAGATG AGATTCAGCA GCGACTGGCA CAGTTTCTAG AGCACTGGAA GGAGCTGAAG
    GAACAAGCAG CGGCTCGTGG TCAGAGACTG GAGGAATCTC TTGAGTACCA ACAATTTGTA
    GCCAATGTGG AGGAAGAGGA AGCCTGGATT AATGAGAAGA TGACTCTAGT GGCCAGTGAA
    GACTATGGTG ACACACTGGC TGCCATTCAG GGATTGCTGA AAAAGCACGA GGCATTTGAG
    ACAGACTTTA CAGTGCACAA AGACAGAGTA CATGATGTAT GTGCCAACGG GGAAGACCTG
    ATACAAAAGA ACAATCACCA TGTGGAGAAT ATTAGCGCCA AAATGAAGGG TCTCCGAGGG
    AAGGTGGCAG AACTAGAGAA GGCGGCTGCA CAGCGCAAGG CCAAACTTGA TGAGAATTCA
    GCTTTCCTGC AGTTCAACTG GAAGGCGGAT GTTGTGGAGT CCTGGATCGG TGAAAAAGAG
    AACAGCCTGA AGACTGATGA TTATGGCAGA GACCTGTCCT CTGTGCAGAC CCTTCTCACT
    AAGCAGGAAA CATTTGATGC TGGGCTGCAA GCTTTCCAGC AAGAGGGCAT TACCAACATC
    ACTGCACTGA AAGACCAGCT TCTGGCTGCA AAGCATGTGC AGTCCCGGGC CATAGAGTCT
    CGTCACGCTT CTCTGATGAA GCGGTGGAAC CAGTTGTTGG ATAACTCTGC TGCCCGCAAA
    AAGAAGTTAT TGGAGGCCCA GGAACACTAC AGAAAGGTTG AGGACTTGTT CCTAACATTT
    GCCAAGAAAG CCTCTGCCTT CAACAGCTGG TTTGAGAATG CCGAGGAGGA TTTGACTGAT
    CCTGTTCGCT GCAACTCCCT GGAGGAAATC AAGGCGCTTC GCGAGGCACA TGACGCCTTC
    CGCAATTCCC TCAGCTCCGC ACAGACAGAT TTCAACCAGC TGGCCGAACT GGACAGGCAA
    ATAAAGGGCT ACCGGGTAGC CTCCAATCCC TACACGTGGT TCACGATGGA GGCTTTGGAG
    GAGACATGGC GCAACCTGCA GAAAATCATT AAGGAACGAG AACTTGAGCT ACAAAAAGAA
    CAACGTAGGC AGGAAGAGAA CGATAAGCTC CGGCAAGAGT TTGCTCAGCA CGCCAATGCT
    TTCCACCAGT GGATTCAGGA GACAAGGTCC TGTATGGTGG AAGAATCTGG AACACTGGAG
    TCACAACTTG AAGCTACAAA GAGAAAGCAT CAGGAGATCC GGGCCATGAG GAGTCAACTG
    AAGAAGATAG AAGATCTTGG CGCAGCCATG GAAGAGGCCC TGATCTTGGA TAATAAATAC
    ACAGAACACA GCACTGTTGG GCTGGCACAG CAATGGGACC AACTGGATCA GCTAGGCATG
    AGAATGCAAC ACAACCTAGA GCAACAGATC CAGGCCAGAA ACACAACTGG GGTCACAGAG
    GAAGCACTCA AAGAATTCAG CATGATGTTC AAGCATTTCG ACAAAGACAA GTCTGGCCGC
    CTCAACCACC AAGAATTCAA ATCTTGTCTG CGGTCCCTTG GCTATGATCT GCCCATGGTA
    GAAGAAGGAG AACCAGACCC AGAGTTTGAA GCCATTTTGG ACACTGTAGA TCCCAACAGA
    GATGGGCACG TTTCTCTACA GGAGTACATG GCTTTCATGA TTAGCAGAGA AACTGAAAAC
    GTCAAGTCCA GTGAGGAGAT AGAGAGCGCC TTCCGAGCAC TGAGTACAGA CCAGAAACCT
    TATGTCACAA AGGAAGAACT ATACCAGAAC CTGACACGCG AACAGGCAGA TTACTGCATC
    TCGCATATGA AACCCTACAT GGACAGCAAG GGCCGCGAAC TGCCCTCTGC ATACGACTAC
    GTGGAATTCA CGCGCTCGCT ATTTGTGAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29836
    Clone ID Related Accession (Same CDS sequence) XM_031906807.1 , XM_012967795.2
    Accession Version XM_031906807.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7371bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGGATTCAG GTGGCGTGAA AGTGCTGGAG ACAGCGGAGG ACATCCAGGA AAGGCGCCAG 
    CAAGTCCTAG ACCGATACCT GCGGTTTAAG GAACTCTCTG TACTGAGACG TCAGAAGTTG
    GAGGATTCTT ACCGCTTCCA GTTCTTCCAG CGAGATGCTG ATGAACTGGA GAAATGGATC
    CAGGAGAAAC TCCAGATCGC ATCTGATGAG AACTACAAAG ATCCAACCAA TCTGCAGGGC
    AAATTGCAGA AACACCAGGC TTTTGAGGCA GAAGTACAGG CCAATGCAGG GGCAATTGTC
    AAACTGGACG AAACTGGAAA CCTGATGATT TCAGAAGGAC ATTTTTCATC AGAGACCATC
    AGAACCCGCC TGGTAGAGTT GCATCGTCTG TGGGAGCTGC TGCTCTTAAA GATGCAAGAG
    AAAGGGGTTA AATTACAACA GGCGCAGAAG CTTGTTCAGT ATCTACGGGA ATGTGAAGAT
    GTCCTAGACT GGATTAATGA CAAGGAGGCT ATTGTAACAT CTGAGGAGCT TGGTCAGGAT
    TTGGAACATG TGGAGGTCTT ACAGAAGAAG TTTGAAGAGT TTCAGACTGA TCTGGCAGCT
    CATGAAGAAA GAGTGAATGA AGTCAACCAG TTTGCAAGTA AACTGGCCCA GGAGGAACAC
    CCGGAGCTGG AGCTAATAAA GACCAAGCAG GATGAAGTCA ATGCAAGTTG GCAGCGCCTC
    AAAGGGCTGG CACTTCAGAG ACAAGGGAAG TTATTTGGAG CTGCAGAAGT TCAGCGTTTT
    AACAGAGATG TTGATGAAAC CATTGGATGG ATAAGAGAGA AGGAGCAGCT GATGGCATCT
    GATGACTTTG GCCGAGACCT GGCTAGCGTT CAAGCTCTCC TACGTAAACA CGAGGGTCTG
    GAGAGAGACC TTGCAGCACT GGAGGAAAAG GTAAAGGCCT TGTGTGCAGA ATCAGACCGT
    CTGCAGCAAT CCCATCCCAT GAACGCCCCT CAAATTCAAG TGAAAGGAGA AGAACTGATA
    ACCAACTGGG AGCAAATACG CACATTGGCA GCAGAACGAC ATGCTCGACT CAATGACTCT
    TACAAGCTTC AGCGTTTCCT TGCAGATTTT AGAGATCTGA CTAGCTGGGT GACTGAAATG
    AAAGCTCTGA TTAATGCCGA TGAGCTTGCC AACGATGTGG CTGGCGCAGA GGCTCTGCTT
    GACCGACACC AGGAACATAA GGGGGAAATT GATGCTCATG AAGATAGCTT TAAAGCTGCA
    GATGCTGCAG GTCAGGCCCT TCTGAACGGT GGCCACTACG CCTCAGATGA AGTCCGAGAA
    AAGCTTACTA TCTTAGCAGA AGAAAGGGCA GCTCTTCTAG AGCTTTGGGA GCTCCGTCGT
    CAGCAGTATG AACAGTGCAT GGACCTGCAG CTTTTTTACA GAGACACCGA ACAGGTGGAC
    AACTGGATGA GCAAACAGGA AGCTTTCCTG CTTAACGAGG ACTTGGGTGA TTCTCTAGAC
    AGTGTGGAAG CTCTTCTGAA AAAACATGAG GACTTTGAGA AGTCTCTTAG CGCCCAGGAA
    GAGAAAATCA CAGCTCTGGA TGAGTTTGCT ACAAAACTAA TTCAAAATAA CCACTATGCC
    AAGGAAGATG TGGCTACCCG CAGAGATGCT CTGCTGAGCC GCCGCAATGC ACTTCATGAA
    CGTGCACTCT ACCGCCGTAC TCAACTGGCA GACTCCTTTC ACCTGCAGCA GTTCTTTAGG
    GACTCTGACG AGCTGAAAAG TTGGATTAAT GAAAAGATGA AGACTGCAAC GGATGAGGCA
    TACAAGGATC CCTCCAACCT CCAGGGTAAG GTGCAGAAAC ACCAAGCTTT TGAGGCTGAA
    CTCTCCGCTA ACCAAAGCCG TATAGATGCC CTGGAGAATT CTGGGCAGAA ATTGATTGAT
    GTCAACCATT ATGCATCAGA TGAGGTGGCA GCACGTATGA GTGAGGTCAT CACTTTGTGG
    AAGAAGCTGC TGGAGGCCAC TGAATTGAAG GGGATCAAGC TGCGCGAAGC CAACCAGCAG
    CAGCAGTTCA ACCGTAATGT GGAGGATATA GAGCTGTGGC TGTATGAGGT TGAGGGGCAT
    TTGGCTTCAG ATGACTATGG AAAAGACCTG ACCAATGTTC AAAACTTGCA GAAAAAACAC
    GCCTTGCTGG AGGCTGATGT TGCAGCTCAC CAGGACCGTA TCGATGGCAT CACTATCCAA
    GCCCGTCAGT TCCAAGAAGC TGGTCATTTT GATGCTGACA ATATCAGGAA GAAACAGGAG
    GCCCTTGTTA ATAGATATGA AGCTTTGAAA GAACCCATGG TTGCACGCAA GCAGAAGCTG
    TCAGACTCTC TGCGTCTTCA ACAGCTTTTC AGGGATGTTG AAGATGAGGA AACCTGGATA
    AGGGAGAAGG AACCCATTGC AGCGTCCACC AACAGAGGCA AGGATTTGAT TGGAGTCCAG
    AATCTCCTGA AGAAACACCA GGCTTTGCAG GCTGAAATTG CAGGGCACGA ACCACGAATT
    AAAGCTGTTA CCCAGAAAGG AGAATCTATG ATCTCTGAAG GCCACTTTGC TTCTGATGAA
    GTGAAGGGCA AATTAAGGGA GCTGAATGAT AAGTGGGCCA CCCTGAAGAA CAAAGCATCT
    CAGCGCCGAC AGGACCTTGA AGATTCTCTG CAAGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCAGAGT CCTGGATGAG AGAGAAGGAA CCTATTGTTG GAAGCACCGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC CCTTCTGAAG AAACATGAAG CGCTTATGTC AGATCTGCGA
    GCATACGGAA GCAGTATTCA GGGACTTAGA GAGCAGGCCC AGGCCTGCAG GCAACAAGTT
    GCTCCAACTG ATGATGAAAC TGGAAAGGAG CTGGTGCTGG CATTGTATGA TTATCAAGAG
    AAGAGCCCTA GGGAGGTGAC AATGAAGAAA GGAGACATCT TGACCTTGCT AAACAGCACA
    AATAAGGATT GGTGGAAGGT GGAAGTGAAT GATCGTCAGG GCTTTGTACC TGCTGCGTAT
    GTGAAAAAGC TGGACCCAGC ACAGTCCGCA TCCCGGGAAA ACTTGCTGGA GGAACAGGGA
    AGCATCGCTC TCCGTCAGGA GCAGATCGAT GGCCAGTATC ATAATATTTT GGAGATGGGA
    GAGAAGCGCA AAGATATGTT AGAAAAGAGC TGCAAGAAGT TTATGCTGTT CCGTGAGGCA
    AATGAGCTGC AGCAGTGGAT TAACGAGAAA GAAGCATCAC TCACTAACGA AGAGGTCGGG
    GCTGACCTGG AACAAGTGGA AGTGCTGCAG AAGAAGTTTG ATGATTTCCA GAAGGATCTG
    AAAGCAAATG AGTCACGCTT GAAGGACATC AACAAGGTGG CCGACGAGCT TGAATCTGAA
    GGCCTCATTA CAGAGGAAGT CCAGCCGGTG CAGCATCAGG AGGTGTATGG GATGCAGCGG
    GATGAAGCAG ACTCCAAATC GGCATCTCCA TGGAAGTCTG TTCGCACAGC TGTGCTCGCG
    GTGGCCACCT TCAACTCTAT CAAGGACTTG AACGAGCGCT GGAAATCACT TCAACAGCTG
    GCAGAAGAAA GGAGTCAGCT TCTTGGCAGT GCCCATGAAG TACAGAGATT CCACAGAGAT
    GCTGATGAGA CAAAAGAATG GATCGAAGAG AAGAACCAGG CTTTGAATAC AGACAGCTAT
    GGACATGACT TGGCCAGTGT GCAGGCTCTG CAACGAAAAC ATGAAGGCTT TGAGAGAGAC
    CTCGCCGCTC TGGGAGATAA GGTGAACTCT TTGGGAGAAA CTGCTGAGCG CCTGATCCAG
    TCACACCCTG AGTCTGCAGA TGACATTCAG GAAAAATGCA CAGAGCTGAA TCAGGCCTGG
    AACAGCCTTG GCACCCGGGC AAACCAGCGC AAGGAGAAAC TAGGCGATTC TCATGATCTG
    CAACGCTTCC TAAGTGACTT CAGAGATCTC ATGTCCTGGA TCAATGGAAT TCGGGGCTTG
    GTGTCATCAG ATGAACTTGC TAGAGATGTG ACTGGTGCAG AGGCTTTGCT GGAACGACAC
    CAGGAGCACA GGACTGAGAT TGATGCCAGA GCAGGCACTT TTCAGGCCTT TGAGCAGTTT
    GGGCAGCAAC TTCTGGCTCG TGGTCATTAT GATAGTCCTG AAATCAAGGA GAAGCTGGAT
    ATCTTGGAAC AGGAACGGGC AAGTTTGGAA AAGGCTTGGG TGGAACGCAG AATGATGTTG
    GATCAGTGCT TGGAGCTGCA GCTCTTCAAC AGAGACTGCG AGCAGGCAGA GAACTGGATG
    GCAGCCCGTG AAGCCTTCCT GAACAGTGAG GATAAAGGGG ATTCCCTGGA CAGTGTGGAG
    GCCCTTATCA AGAAACATGA AGACTTTGAT AAAGCCATTA ATGTCCAGGA AGAGAAGATT
    GCTGCCCTCC AGGCCTTCGC CGAACAATTG ATAATTGCTG ACCACTATGC CAAAGGAGAT
    ATTGCCTCCC GTCGCAATGA AGTCCTGGAC AGGTGGCGCC GTCTCAAGGC TCAGATGATT
    GAGAAGAGAT CCAAACTTGG GGAATCTCAG ACTCTGCAGC AGTTCAGCAG GGACGTCGAT
    GAGATCGAAG CCTGGATCAG CGAGAAATTG CAGACCGCCA GTGACGAGTC CTACAAAGAC
    CCCACAAACA TCCAGCTTTC CAAATTGCTG AGCAAACACC AGAAGCACCA GGCTTTCGAA
    GCTGAGCTCC ATGCCAACGC TGACCGTATT CGTGGAGTAG TAGATATGGG GAACTCCCTT
    ATCGAACGAG GTGCCTGTGC TGGCAGCGAG GATGCGGTGA AGGCTCGCCT AAGCGCACTC
    GCTGATCAAT GGCAGTTCCT TGTCAGCAAA TCCGCAGAGA AGAGCCAGAA ACTGAAGGAG
    GCAAACAAGC AGCAGAACTT TAACACTGGC ATCAAAGACT TTGATTTCTG GTTAACAGAG
    GTGGAGGCTT TGCTGGCATC TGAAGACTAT GGGAAGGACT TGGCATCTGT TAACAACCTG
    CTGAAGAAAC ACCAGCTTCT GGAAGCTGAC ATCTCTGCTC ATGAGGACCG CCTTAAAGAT
    CTTAATGGGC AGGCTGATAG CCTGATGACC AGCAGCGCCT TTGACACCAC CCAAGTAAAG
    GAGAAAAGGG ACGCCATCAA CGACCGCTTC CAGCGTATCA AAAGCATGGC CACCGCACGC
    CGCAGCAGGC TGAATGAGTC CCACCGTCTG CACCAGTTCT TCAGAGATCT TGATGATGAG
    GAATCATGGA TCAAGGAAAA GAAACTTTTG GTCGGATCTG ATGACTATGG ACGTGACTTG
    ACTGGAGTGC AAAATCTGAG GAAGAAACAC AAGAGATTAG AAGCTGAACT GGCAGCTCAT
    GAGCCAGCCA TTCAGGGTGT GCTGGACACT GGGAAGAAGC TTGCAGATGA CAACACTATT
    GGGAAAGATG AGATTCAGCA GCGACTGGCA CAGTTTCTAG AGCACTGGAA GGAGCTGAAG
    GAACAAGCAG CGGCTCGTGG TCAGAGACTG GAGGAATCTC TTGAGTACCA ACAATTTGTA
    GCCAATGTGG AGGAAGAGGA AGCCTGGATT AATGAGAAGA TGACTCTAGT GGCCAGTGAA
    GACTATGGTG ACACACTGGC TGCCATTCAG GGATTGCTGA AAAAGCACGA GGCATTTGAG
    ACAGACTTTA CAGTGCACAA AGACAGAGTA CATGATGTAT GTGCCAACGG GGAAGACCTG
    ATACAAAAGA ACAATCACCA TGTGGAGAAT ATTAGCGCCA AAATGAAGGG TCTCCGAGGG
    AAGGTGGCAG AACTAGAGAA GGCGGCTGCA CAGCGCAAGG CCAAACTTGA TGAGAATTCA
    GCTTTCCTGC AGTTCAACTG GAAGGCGGAT GTTGTGGAGT CCTGGATCGG TGAAAAAGAG
    AACAGCCTGA AGACTGATGA TTATGGCAGA GACCTGTCCT CTGTGCAGAC CCTTCTCACT
    AAGCAGGAAA CATTTGATGC TGGGCTGCAA GCTTTCCAGC AAGAGGGCAT TACCAACATC
    ACTGCACTGA AAGACCAGCT TCTGGCTGCA AAGCATGTGC AGTCCCGGGC CATAGAGTCT
    CGTCACGCTT CTCTGATGAA GCGGTGGAAC CAGTTGTTGG ATAACTCTGC TGCCCGCAAA
    AAGAAGTTAT TGGAGGCCCA GGAACACTAC AGAAAGGTTG AGGACTTGTT CCTAACATTT
    GCCAAGAAAG CCTCTGCCTT CAACAGCTGG TTTGAGAATG CCGAGGAGGA TTTGACTGAT
    CCTGTTCGCT GCAACTCCCT GGAGGAAATC AAGGCGCTTC GCGAGGCACA TGACGCCTTC
    CGCAATTCCC TCAGCTCCGC ACAGACAGAT TTCAACCAGC TGGCCGAACT GGACAGGCAA
    ATAAAGGGCT ACCGGGTAGC CTCCAATCCC TACACGTGGT TCACGATGGA GGCTTTGGAG
    GAGACATGGC GCAACCTGCA GAAAATCATT AAGGAACGAG AACTTGAGCT ACAAAAAGAA
    CAACGTAGGC AGGAAGAGAA CGATAAGCTC CGGCAAGAGT TTGCTCAGCA CGCCAATGCT
    TTCCACCAGT GGATTCAGGA GACAAGGACC TACCTTCTGG ACGGGTCCTG TATGGTGGAA
    GAATCTGGAA CACTGGAGTC ACAACTTGAA GCTACAAAGA GAAAGCATCA GGAGATCCGG
    GCCATGAGGA GTCAACTGAA GAAGATAGAA GATCTTGGCG CAGCCATGGA AGAGGCCCTG
    ATCTTGGATA ATAAATACAC AGAACACAGC ACTGTTGGGC TGGCACAGCA ATGGGACCAA
    CTGGATCAGC TAGGCATGAG AATGCAACAC AACCTAGAGC AACAGATCCA GGCCAGAAAC
    ACAACTGGGG TCACAGAGGA AGCACTCAAA GAATTCAGCA TGATGTTCAA GCATTTCGAC
    AAAGACAAGT CTGGCCGCCT CAACCACCAA GAATTCAAAT CTTGTCTGCG GTCCCTTGGC
    TATGATCTGC CCATGGTAGA AGAAGGAGAA CCAGACCCAG AGTTTGAAGC CATTTTGGAC
    ACTGTAGATC CCAACAGAGA TGGGCACGTT TCTCTACAGG AGTACATGGC TTTCATGATT
    AGCAGAGAAA CTGAAAACGT CAAGTCCAGT GAGGAGATAG AGAGCGCCTT CCGAGCACTG
    AGTACAGACC AGAAACCTTA TGTCACAAAG GAAGAACTAT ACCAGAACCT GACACGCGAA
    CAGGCAGATT ACTGCATCTC GCATATGAAA CCCTACATGG ACAGCAAGGG CCGCGAACTG
    CCCTCTGCAT ACGACTACGT GGAATTCACG CGCTCGCTAT TTGTGAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031762667.1
    CDS4..7374
    Translation

    Target ORF information:

    RefSeq Version XM_031906807.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis spectrin, alpha, non-erythrocytic 1 (sptan1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031906807.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGGATTCAG GTGGCGTGAA AGTGCTGGAG ACAGCGGAGG ACATCCAGGA AAGGCGCCAG 
    CAAGTCCTAG ACCGATACCT GCGGTTTAAG GAACTCTCTG TACTGAGACG TCAGAAGTTG
    GAGGATTCTT ACCGCTTCCA GTTCTTCCAG CGAGATGCTG ATGAACTGGA GAAATGGATC
    CAGGAGAAAC TCCAGATCGC ATCTGATGAG AACTACAAAG ATCCAACCAA TCTGCAGGGC
    AAATTGCAGA AACACCAGGC TTTTGAGGCA GAAGTACAGG CCAATGCAGG GGCAATTGTC
    AAACTGGACG AAACTGGAAA CCTGATGATT TCAGAAGGAC ATTTTTCATC AGAGACCATC
    AGAACCCGCC TGGTAGAGTT GCATCGTCTG TGGGAGCTGC TGCTCTTAAA GATGCAAGAG
    AAAGGGGTTA AATTACAACA GGCGCAGAAG CTTGTTCAGT ATCTACGGGA ATGTGAAGAT
    GTCCTAGACT GGATTAATGA CAAGGAGGCT ATTGTAACAT CTGAGGAGCT TGGTCAGGAT
    TTGGAACATG TGGAGGTCTT ACAGAAGAAG TTTGAAGAGT TTCAGACTGA TCTGGCAGCT
    CATGAAGAAA GAGTGAATGA AGTCAACCAG TTTGCAAGTA AACTGGCCCA GGAGGAACAC
    CCGGAGCTGG AGCTAATAAA GACCAAGCAG GATGAAGTCA ATGCAAGTTG GCAGCGCCTC
    AAAGGGCTGG CACTTCAGAG ACAAGGGAAG TTATTTGGAG CTGCAGAAGT TCAGCGTTTT
    AACAGAGATG TTGATGAAAC CATTGGATGG ATAAGAGAGA AGGAGCAGCT GATGGCATCT
    GATGACTTTG GCCGAGACCT GGCTAGCGTT CAAGCTCTCC TACGTAAACA CGAGGGTCTG
    GAGAGAGACC TTGCAGCACT GGAGGAAAAG GTAAAGGCCT TGTGTGCAGA ATCAGACCGT
    CTGCAGCAAT CCCATCCCAT GAACGCCCCT CAAATTCAAG TGAAAGGAGA AGAACTGATA
    ACCAACTGGG AGCAAATACG CACATTGGCA GCAGAACGAC ATGCTCGACT CAATGACTCT
    TACAAGCTTC AGCGTTTCCT TGCAGATTTT AGAGATCTGA CTAGCTGGGT GACTGAAATG
    AAAGCTCTGA TTAATGCCGA TGAGCTTGCC AACGATGTGG CTGGCGCAGA GGCTCTGCTT
    GACCGACACC AGGAACATAA GGGGGAAATT GATGCTCATG AAGATAGCTT TAAAGCTGCA
    GATGCTGCAG GTCAGGCCCT TCTGAACGGT GGCCACTACG CCTCAGATGA AGTCCGAGAA
    AAGCTTACTA TCTTAGCAGA AGAAAGGGCA GCTCTTCTAG AGCTTTGGGA GCTCCGTCGT
    CAGCAGTATG AACAGTGCAT GGACCTGCAG CTTTTTTACA GAGACACCGA ACAGGTGGAC
    AACTGGATGA GCAAACAGGA AGCTTTCCTG CTTAACGAGG ACTTGGGTGA TTCTCTAGAC
    AGTGTGGAAG CTCTTCTGAA AAAACATGAG GACTTTGAGA AGTCTCTTAG CGCCCAGGAA
    GAGAAAATCA CAGCTCTGGA TGAGTTTGCT ACAAAACTAA TTCAAAATAA CCACTATGCC
    AAGGAAGATG TGGCTACCCG CAGAGATGCT CTGCTGAGCC GCCGCAATGC ACTTCATGAA
    CGTGCACTCT ACCGCCGTAC TCAACTGGCA GACTCCTTTC ACCTGCAGCA GTTCTTTAGG
    GACTCTGACG AGCTGAAAAG TTGGATTAAT GAAAAGATGA AGACTGCAAC GGATGAGGCA
    TACAAGGATC CCTCCAACCT CCAGGGTAAG GTGCAGAAAC ACCAAGCTTT TGAGGCTGAA
    CTCTCCGCTA ACCAAAGCCG TATAGATGCC CTGGAGAATT CTGGGCAGAA ATTGATTGAT
    GTCAACCATT ATGCATCAGA TGAGGTGGCA GCACGTATGA GTGAGGTCAT CACTTTGTGG
    AAGAAGCTGC TGGAGGCCAC TGAATTGAAG GGGATCAAGC TGCGCGAAGC CAACCAGCAG
    CAGCAGTTCA ACCGTAATGT GGAGGATATA GAGCTGTGGC TGTATGAGGT TGAGGGGCAT
    TTGGCTTCAG ATGACTATGG AAAAGACCTG ACCAATGTTC AAAACTTGCA GAAAAAACAC
    GCCTTGCTGG AGGCTGATGT TGCAGCTCAC CAGGACCGTA TCGATGGCAT CACTATCCAA
    GCCCGTCAGT TCCAAGAAGC TGGTCATTTT GATGCTGACA ATATCAGGAA GAAACAGGAG
    GCCCTTGTTA ATAGATATGA AGCTTTGAAA GAACCCATGG TTGCACGCAA GCAGAAGCTG
    TCAGACTCTC TGCGTCTTCA ACAGCTTTTC AGGGATGTTG AAGATGAGGA AACCTGGATA
    AGGGAGAAGG AACCCATTGC AGCGTCCACC AACAGAGGCA AGGATTTGAT TGGAGTCCAG
    AATCTCCTGA AGAAACACCA GGCTTTGCAG GCTGAAATTG CAGGGCACGA ACCACGAATT
    AAAGCTGTTA CCCAGAAAGG AGAATCTATG ATCTCTGAAG GCCACTTTGC TTCTGATGAA
    GTGAAGGGCA AATTAAGGGA GCTGAATGAT AAGTGGGCCA CCCTGAAGAA CAAAGCATCT
    CAGCGCCGAC AGGACCTTGA AGATTCTCTG CAAGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCAGAGT CCTGGATGAG AGAGAAGGAA CCTATTGTTG GAAGCACCGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC CCTTCTGAAG AAACATGAAG CGCTTATGTC AGATCTGCGA
    GCATACGGAA GCAGTATTCA GGGACTTAGA GAGCAGGCCC AGGCCTGCAG GCAACAAGTT
    GCTCCAACTG ATGATGAAAC TGGAAAGGAG CTGGTGCTGG CATTGTATGA TTATCAAGAG
    AAGAGCCCTA GGGAGGTGAC AATGAAGAAA GGAGACATCT TGACCTTGCT AAACAGCACA
    AATAAGGATT GGTGGAAGGT GGAAGTGAAT GATCGTCAGG GCTTTGTACC TGCTGCGTAT
    GTGAAAAAGC TGGACCCAGC ACAGTCCGCA TCCCGGGAAA ACTTGCTGGA GGAACAGGGA
    AGCATCGCTC TCCGTCAGGA GCAGATCGAT GGCCAGTATC ATAATATTTT GGAGATGGGA
    GAGAAGCGCA AAGATATGTT AGAAAAGAGC TGCAAGAAGT TTATGCTGTT CCGTGAGGCA
    AATGAGCTGC AGCAGTGGAT TAACGAGAAA GAAGCATCAC TCACTAACGA AGAGGTCGGG
    GCTGACCTGG AACAAGTGGA AGTGCTGCAG AAGAAGTTTG ATGATTTCCA GAAGGATCTG
    AAAGCAAATG AGTCACGCTT GAAGGACATC AACAAGGTGG CCGACGAGCT TGAATCTGAA
    GGCCTCATTA CAGAGGAAGT CCAGCCGGTG CAGCATCAGG AGGTGTATGG GATGCAGCGG
    GATGAAGCAG ACTCCAAATC GGCATCTCCA TGGAAGTCTG TTCGCACAGC TGTGCTCGCG
    GTGGCCACCT TCAACTCTAT CAAGGACTTG AACGAGCGCT GGAAATCACT TCAACAGCTG
    GCAGAAGAAA GGAGTCAGCT TCTTGGCAGT GCCCATGAAG TACAGAGATT CCACAGAGAT
    GCTGATGAGA CAAAAGAATG GATCGAAGAG AAGAACCAGG CTTTGAATAC AGACAGCTAT
    GGACATGACT TGGCCAGTGT GCAGGCTCTG CAACGAAAAC ATGAAGGCTT TGAGAGAGAC
    CTCGCCGCTC TGGGAGATAA GGTGAACTCT TTGGGAGAAA CTGCTGAGCG CCTGATCCAG
    TCACACCCTG AGTCTGCAGA TGACATTCAG GAAAAATGCA CAGAGCTGAA TCAGGCCTGG
    AACAGCCTTG GCACCCGGGC AAACCAGCGC AAGGAGAAAC TAGGCGATTC TCATGATCTG
    CAACGCTTCC TAAGTGACTT CAGAGATCTC ATGTCCTGGA TCAATGGAAT TCGGGGCTTG
    GTGTCATCAG ATGAACTTGC TAGAGATGTG ACTGGTGCAG AGGCTTTGCT GGAACGACAC
    CAGGAGCACA GGACTGAGAT TGATGCCAGA GCAGGCACTT TTCAGGCCTT TGAGCAGTTT
    GGGCAGCAAC TTCTGGCTCG TGGTCATTAT GATAGTCCTG AAATCAAGGA GAAGCTGGAT
    ATCTTGGAAC AGGAACGGGC AAGTTTGGAA AAGGCTTGGG TGGAACGCAG AATGATGTTG
    GATCAGTGCT TGGAGCTGCA GCTCTTCAAC AGAGACTGCG AGCAGGCAGA GAACTGGATG
    GCAGCCCGTG AAGCCTTCCT GAACAGTGAG GATAAAGGGG ATTCCCTGGA CAGTGTGGAG
    GCCCTTATCA AGAAACATGA AGACTTTGAT AAAGCCATTA ATGTCCAGGA AGAGAAGATT
    GCTGCCCTCC AGGCCTTCGC CGAACAATTG ATAATTGCTG ACCACTATGC CAAAGGAGAT
    ATTGCCTCCC GTCGCAATGA AGTCCTGGAC AGGTGGCGCC GTCTCAAGGC TCAGATGATT
    GAGAAGAGAT CCAAACTTGG GGAATCTCAG ACTCTGCAGC AGTTCAGCAG GGACGTCGAT
    GAGATCGAAG CCTGGATCAG CGAGAAATTG CAGACCGCCA GTGACGAGTC CTACAAAGAC
    CCCACAAACA TCCAGCTTTC CAAATTGCTG AGCAAACACC AGAAGCACCA GGCTTTCGAA
    GCTGAGCTCC ATGCCAACGC TGACCGTATT CGTGGAGTAG TAGATATGGG GAACTCCCTT
    ATCGAACGAG GTGCCTGTGC TGGCAGCGAG GATGCGGTGA AGGCTCGCCT AAGCGCACTC
    GCTGATCAAT GGCAGTTCCT TGTCAGCAAA TCCGCAGAGA AGAGCCAGAA ACTGAAGGAG
    GCAAACAAGC AGCAGAACTT TAACACTGGC ATCAAAGACT TTGATTTCTG GTTAACAGAG
    GTGGAGGCTT TGCTGGCATC TGAAGACTAT GGGAAGGACT TGGCATCTGT TAACAACCTG
    CTGAAGAAAC ACCAGCTTCT GGAAGCTGAC ATCTCTGCTC ATGAGGACCG CCTTAAAGAT
    CTTAATGGGC AGGCTGATAG CCTGATGACC AGCAGCGCCT TTGACACCAC CCAAGTAAAG
    GAGAAAAGGG ACGCCATCAA CGACCGCTTC CAGCGTATCA AAAGCATGGC CACCGCACGC
    CGCAGCAGGC TGAATGAGTC CCACCGTCTG CACCAGTTCT TCAGAGATCT TGATGATGAG
    GAATCATGGA TCAAGGAAAA GAAACTTTTG GTCGGATCTG ATGACTATGG ACGTGACTTG
    ACTGGAGTGC AAAATCTGAG GAAGAAACAC AAGAGATTAG AAGCTGAACT GGCAGCTCAT
    GAGCCAGCCA TTCAGGGTGT GCTGGACACT GGGAAGAAGC TTGCAGATGA CAACACTATT
    GGGAAAGATG AGATTCAGCA GCGACTGGCA CAGTTTCTAG AGCACTGGAA GGAGCTGAAG
    GAACAAGCAG CGGCTCGTGG TCAGAGACTG GAGGAATCTC TTGAGTACCA ACAATTTGTA
    GCCAATGTGG AGGAAGAGGA AGCCTGGATT AATGAGAAGA TGACTCTAGT GGCCAGTGAA
    GACTATGGTG ACACACTGGC TGCCATTCAG GGATTGCTGA AAAAGCACGA GGCATTTGAG
    ACAGACTTTA CAGTGCACAA AGACAGAGTA CATGATGTAT GTGCCAACGG GGAAGACCTG
    ATACAAAAGA ACAATCACCA TGTGGAGAAT ATTAGCGCCA AAATGAAGGG TCTCCGAGGG
    AAGGTGGCAG AACTAGAGAA GGCGGCTGCA CAGCGCAAGG CCAAACTTGA TGAGAATTCA
    GCTTTCCTGC AGTTCAACTG GAAGGCGGAT GTTGTGGAGT CCTGGATCGG TGAAAAAGAG
    AACAGCCTGA AGACTGATGA TTATGGCAGA GACCTGTCCT CTGTGCAGAC CCTTCTCACT
    AAGCAGGAAA CATTTGATGC TGGGCTGCAA GCTTTCCAGC AAGAGGGCAT TACCAACATC
    ACTGCACTGA AAGACCAGCT TCTGGCTGCA AAGCATGTGC AGTCCCGGGC CATAGAGTCT
    CGTCACGCTT CTCTGATGAA GCGGTGGAAC CAGTTGTTGG ATAACTCTGC TGCCCGCAAA
    AAGAAGTTAT TGGAGGCCCA GGAACACTAC AGAAAGGTTG AGGACTTGTT CCTAACATTT
    GCCAAGAAAG CCTCTGCCTT CAACAGCTGG TTTGAGAATG CCGAGGAGGA TTTGACTGAT
    CCTGTTCGCT GCAACTCCCT GGAGGAAATC AAGGCGCTTC GCGAGGCACA TGACGCCTTC
    CGCAATTCCC TCAGCTCCGC ACAGACAGAT TTCAACCAGC TGGCCGAACT GGACAGGCAA
    ATAAAGGGCT ACCGGGTAGC CTCCAATCCC TACACGTGGT TCACGATGGA GGCTTTGGAG
    GAGACATGGC GCAACCTGCA GAAAATCATT AAGGAACGAG AACTTGAGCT ACAAAAAGAA
    CAACGTAGGC AGGAAGAGAA CGATAAGCTC CGGCAAGAGT TTGCTCAGCA CGCCAATGCT
    TTCCACCAGT GGATTCAGGA GACAAGGACC TACCTTCTGG ACGGGTCCTG TATGGTGGAA
    GAATCTGGAA CACTGGAGTC ACAACTTGAA GCTACAAAGA GAAAGCATCA GGAGATCCGG
    GCCATGAGGA GTCAACTGAA GAAGATAGAA GATCTTGGCG CAGCCATGGA AGAGGCCCTG
    ATCTTGGATA ATAAATACAC AGAACACAGC ACTGTTGGGC TGGCACAGCA ATGGGACCAA
    CTGGATCAGC TAGGCATGAG AATGCAACAC AACCTAGAGC AACAGATCCA GGCCAGAAAC
    ACAACTGGGG TCACAGAGGA AGCACTCAAA GAATTCAGCA TGATGTTCAA GCATTTCGAC
    AAAGACAAGT CTGGCCGCCT CAACCACCAA GAATTCAAAT CTTGTCTGCG GTCCCTTGGC
    TATGATCTGC CCATGGTAGA AGAAGGAGAA CCAGACCCAG AGTTTGAAGC CATTTTGGAC
    ACTGTAGATC CCAACAGAGA TGGGCACGTT TCTCTACAGG AGTACATGGC TTTCATGATT
    AGCAGAGAAA CTGAAAACGT CAAGTCCAGT GAGGAGATAG AGAGCGCCTT CCGAGCACTG
    AGTACAGACC AGAAACCTTA TGTCACAAAG GAAGAACTAT ACCAGAACCT GACACGCGAA
    CAGGCAGATT ACTGCATCTC GCATATGAAA CCCTACATGG ACAGCAAGGG CCGCGAACTG
    CCCTCTGCAT ACGACTACGT GGAATTCACG CGCTCGCTAT TTGTGAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29837
    Clone ID Related Accession (Same CDS sequence) XM_012967796.2 , XM_031906808.1
    Accession Version XM_031906808.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7356bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGGATTCAG GTGGCGTGAA AGTGCTGGAG ACAGCGGAGG ACATCCAGGA AAGGCGCCAG 
    CAAGTCCTAG ACCGATACCT GCGGTTTAAG GAACTCTCTG TACTGAGACG TCAGAAGTTG
    GAGGATTCTT ACCGCTTCCA GTTCTTCCAG CGAGATGCTG ATGAACTGGA GAAATGGATC
    CAGGAGAAAC TCCAGATCGC ATCTGATGAG AACTACAAAG ATCCAACCAA TCTGCAGGGC
    AAATTGCAGA AACACCAGGC TTTTGAGGCA GAAGTACAGG CCAATGCAGG GGCAATTGTC
    AAACTGGACG AAACTGGAAA CCTGATGATT TCAGAAGGAC ATTTTTCATC AGAGACCATC
    AGAACCCGCC TGGTAGAGTT GCATCGTCTG TGGGAGCTGC TGCTCTTAAA GATGCAAGAG
    AAAGGGGTTA AATTACAACA GGCGCAGAAG CTTGTTCAGT ATCTACGGGA ATGTGAAGAT
    GTCCTAGACT GGATTAATGA CAAGGAGGCT ATTGTAACAT CTGAGGAGCT TGGTCAGGAT
    TTGGAACATG TGGAGGTCTT ACAGAAGAAG TTTGAAGAGT TTCAGACTGA TCTGGCAGCT
    CATGAAGAAA GAGTGAATGA AGTCAACCAG TTTGCAAGTA AACTGGCCCA GGAGGAACAC
    CCGGAGCTGG AGCTAATAAA GACCAAGCAG GATGAAGTCA ATGCAAGTTG GCAGCGCCTC
    AAAGGGCTGG CACTTCAGAG ACAAGGGAAG TTATTTGGAG CTGCAGAAGT TCAGCGTTTT
    AACAGAGATG TTGATGAAAC CATTGGATGG ATAAGAGAGA AGGAGCAGCT GATGGCATCT
    GATGACTTTG GCCGAGACCT GGCTAGCGTT CAAGCTCTCC TACGTAAACA CGAGGGTCTG
    GAGAGAGACC TTGCAGCACT GGAGGAAAAG GTAAAGGCCT TGTGTGCAGA ATCAGACCGT
    CTGCAGCAAT CCCATCCCAT GAACGCCCCT CAAATTCAAG TGAAAGGAGA AGAACTGATA
    ACCAACTGGG AGCAAATACG CACATTGGCA GCAGAACGAC ATGCTCGACT CAATGACTCT
    TACAAGCTTC AGCGTTTCCT TGCAGATTTT AGAGATCTGA CTAGCTGGGT GACTGAAATG
    AAAGCTCTGA TTAATGCCGA TGAGCTTGCC AACGATGTGG CTGGCGCAGA GGCTCTGCTT
    GACCGACACC AGGAACATAA GGGGGAAATT GATGCTCATG AAGATAGCTT TAAAGCTGCA
    GATGCTGCAG GTCAGGCCCT TCTGAACGGT GGCCACTACG CCTCAGATGA AGTCCGAGAA
    AAGCTTACTA TCTTAGCAGA AGAAAGGGCA GCTCTTCTAG AGCTTTGGGA GCTCCGTCGT
    CAGCAGTATG AACAGTGCAT GGACCTGCAG CTTTTTTACA GAGACACCGA ACAGGTGGAC
    AACTGGATGA GCAAACAGGA AGCTTTCCTG CTTAACGAGG ACTTGGGTGA TTCTCTAGAC
    AGTGTGGAAG CTCTTCTGAA AAAACATGAG GACTTTGAGA AGTCTCTTAG CGCCCAGGAA
    GAGAAAATCA CAGCTCTGGA TGAGTTTGCT ACAAAACTAA TTCAAAATAA CCACTATGCC
    AAGGAAGATG TGGCTACCCG CAGAGATGCT CTGCTGAGCC GCCGCAATGC ACTTCATGAA
    CGTGCACTCT ACCGCCGTAC TCAACTGGCA GACTCCTTTC ACCTGCAGCA GTTCTTTAGG
    GACTCTGACG AGCTGAAAAG TTGGATTAAT GAAAAGATGA AGACTGCAAC GGATGAGGCA
    TACAAGGATC CCTCCAACCT CCAGGGTAAG GTGCAGAAAC ACCAAGCTTT TGAGGCTGAA
    CTCTCCGCTA ACCAAAGCCG TATAGATGCC CTGGAGAATT CTGGGCAGAA ATTGATTGAT
    GTCAACCATT ATGCATCAGA TGAGGTGGCA GCACGTATGA GTGAGGTCAT CACTTTGTGG
    AAGAAGCTGC TGGAGGCCAC TGAATTGAAG GGGATCAAGC TGCGCGAAGC CAACCAGCAG
    CAGCAGTTCA ACCGTAATGT GGAGGATATA GAGCTGTGGC TGTATGAGGT TGAGGGGCAT
    TTGGCTTCAG ATGACTATGG AAAAGACCTG ACCAATGTTC AAAACTTGCA GAAAAAACAC
    GCCTTGCTGG AGGCTGATGT TGCAGCTCAC CAGGACCGTA TCGATGGCAT CACTATCCAA
    GCCCGTCAGT TCCAAGAAGC TGGTCATTTT GATGCTGACA ATATCAGGAA GAAACAGGAG
    GCCCTTGTTA ATAGATATGA AGCTTTGAAA GAACCCATGG TTGCACGCAA GCAGAAGCTG
    TCAGACTCTC TGCGTCTTCA ACAGCTTTTC AGGGATGTTG AAGATGAGGA AACCTGGATA
    AGGGAGAAGG AACCCATTGC AGCGTCCACC AACAGAGGCA AGGATTTGAT TGGAGTCCAG
    AATCTCCTGA AGAAACACCA GGCTTTGCAG GCTGAAATTG CAGGGCACGA ACCACGAATT
    AAAGCTGTTA CCCAGAAAGG AGAATCTATG ATCTCTGAAG GCCACTTTGC TTCTGATGAA
    GTGAAGGGCA AATTAAGGGA GCTGAATGAT AAGTGGGCCA CCCTGAAGAA CAAAGCATCT
    CAGCGCCGAC AGGACCTTGA AGATTCTCTG CAAGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCAGAGT CCTGGATGAG AGAGAAGGAA CCTATTGTTG GAAGCACCGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC CCTTCTGAAG AAACATGAAG CGCTTATGTC AGATCTGCGA
    GCATACGGAA GCAGTATTCA GGGACTTAGA GAGCAGGCCC AGGCCTGCAG GCAACAAGTT
    GCTCCAACTG ATGATGAAAC TGGAAAGGAG CTGGTGCTGG CATTGTATGA TTATCAAGAG
    AAGAGCCCTA GGGAGGTGAC AATGAAGAAA GGAGACATCT TGACCTTGCT AAACAGCACA
    AATAAGGATT GGTGGAAGGT GGAAGTGAAT GATCGTCAGG GCTTTGTACC TGCTGCGTAT
    GTGAAAAAGC TGGACCCAGC ACAGTCCGCA TCCCGGGAAA ACTTGCTGGA GGAACAGGGA
    AGCATCGCTC TCCGTCAGGA GCAGATCGAT GGCCAGTATC ATAATATTTT GGAGATGGGA
    GAGAAGCGCA AAGATATGTT AGAAAAGAGC TGCAAGAAGT TTATGCTGTT CCGTGAGGCA
    AATGAGCTGC AGCAGTGGAT TAACGAGAAA GAAGCATCAC TCACTAACGA AGAGGTCGGG
    GCTGACCTGG AACAAGTGGA AGTGCTGCAG AAGAAGTTTG ATGATTTCCA GAAGGATCTG
    AAAGCAAATG AGTCACGCTT GAAGGACATC AACAAGGTGG CCGACGAGCT TGAATCTGAA
    GGCCTCATTA CAGAGGAAGT CCAGCCGGTG CAGCATCAGG AGGTGTATGG GATGCAGCGG
    GATGAAGCAG ACTCCAAATC GGCATCTCCA TGGAAGTCTG TTCGCACAGC TGTGCTCGCG
    GTGGCCACCT TCAACTCTAT CAAGGACTTG AACGAGCGCT GGAAATCACT TCAACAGCTG
    GCAGAAGAAA GGAGTCAGCT TCTTGGCAGT GCCCATGAAG TACAGAGATT CCACAGAGAT
    GCTGATGAGA CAAAAGAATG GATCGAAGAG AAGAACCAGG CTTTGAATAC AGACAGCTAT
    GGACATGACT TGGCCAGTGT GCAGGCTCTG CAACGAAAAC ATGAAGGCTT TGAGAGAGAC
    CTCGCCGCTC TGGGAGATAA GGTGAACTCT TTGGGAGAAA CTGCTGAGCG CCTGATCCAG
    TCACACCCTG AGTCTGCAGA TGACATTCAG GAAAAATGCA CAGAGCTGAA TCAGGCCTGG
    AACAGCCTTG GCACCCGGGC AAACCAGCGC AAGGAGAAAC TAGGCGATTC TCATGATCTG
    CAACGCTTCC TAAGTGACTT CAGAGATCTC ATGTCCTGGA TCAATGGAAT TCGGGGCTTG
    GTGTCATCAG ATGAACTTGC TAGAGATGTG ACTGGTGCAG AGGCTTTGCT GGAACGACAC
    CAGGAGCACA GGACTGAGAT TGATGCCAGA GCAGGCACTT TTCAGGCCTT TGAGCAGTTT
    GGGCAGCAAC TTCTGGCTCG TGGTCATTAT GATAGTCCTG AAATCAAGGA GAAGCTGGAT
    ATCTTGGAAC AGGAACGGGC AAGTTTGGAA AAGGCTTGGG TGGAACGCAG AATGATGTTG
    GATCAGTGCT TGGAGCTGCA GCTCTTCAAC AGAGACTGCG AGCAGGCAGA GAACTGGATG
    GCAGCCCGTG AAGCCTTCCT GAACAGTGAG GATAAAGGGG ATTCCCTGGA CAGTGTGGAG
    GCCCTTATCA AGAAACATGA AGACTTTGAT AAAGCCATTA ATGTCCAGGA AGAGAAGATT
    GCTGCCCTCC AGGCCTTCGC CGAACAATTG ATAATTGCTG ACCACTATGC CAAAGGAGAT
    ATTGCCTCCC GTCGCAATGA AGTCCTGGAC AGGTGGCGCC GTCTCAAGGC TCAGATGATT
    GAGAAGAGAT CCAAACTTGG GGAATCTCAG ACTCTGCAGC AGTTCAGCAG GGACGTCGAT
    GAGATCGAAG CCTGGATCAG CGAGAAATTG CAGACCGCCA GTGACGAGTC CTACAAAGAC
    CCCACAAACA TCCAGAGCAA ACACCAGAAG CACCAGGCTT TCGAAGCTGA GCTCCATGCC
    AACGCTGACC GTATTCGTGG AGTAGTAGAT ATGGGGAACT CCCTTATCGA ACGAGGTGCC
    TGTGCTGGCA GCGAGGATGC GGTGAAGGCT CGCCTAAGCG CACTCGCTGA TCAATGGCAG
    TTCCTTGTCA GCAAATCCGC AGAGAAGAGC CAGAAACTGA AGGAGGCAAA CAAGCAGCAG
    AACTTTAACA CTGGCATCAA AGACTTTGAT TTCTGGTTAA CAGAGGTGGA GGCTTTGCTG
    GCATCTGAAG ACTATGGGAA GGACTTGGCA TCTGTTAACA ACCTGCTGAA GAAACACCAG
    CTTCTGGAAG CTGACATCTC TGCTCATGAG GACCGCCTTA AAGATCTTAA TGGGCAGGCT
    GATAGCCTGA TGACCAGCAG CGCCTTTGAC ACCACCCAAG TAAAGGAGAA AAGGGACGCC
    ATCAACGACC GCTTCCAGCG TATCAAAAGC ATGGCCACCG CACGCCGCAG CAGGCTGAAT
    GAGTCCCACC GTCTGCACCA GTTCTTCAGA GATCTTGATG ATGAGGAATC ATGGATCAAG
    GAAAAGAAAC TTTTGGTCGG ATCTGATGAC TATGGACGTG ACTTGACTGG AGTGCAAAAT
    CTGAGGAAGA AACACAAGAG ATTAGAAGCT GAACTGGCAG CTCATGAGCC AGCCATTCAG
    GGTGTGCTGG ACACTGGGAA GAAGCTTGCA GATGACAACA CTATTGGGAA AGATGAGATT
    CAGCAGCGAC TGGCACAGTT TCTAGAGCAC TGGAAGGAGC TGAAGGAACA AGCAGCGGCT
    CGTGGTCAGA GACTGGAGGA ATCTCTTGAG TACCAACAAT TTGTAGCCAA TGTGGAGGAA
    GAGGAAGCCT GGATTAATGA GAAGATGACT CTAGTGGCCA GTGAAGACTA TGGTGACACA
    CTGGCTGCCA TTCAGGGATT GCTGAAAAAG CACGAGGCAT TTGAGACAGA CTTTACAGTG
    CACAAAGACA GAGTACATGA TGTATGTGCC AACGGGGAAG ACCTGATACA AAAGAACAAT
    CACCATGTGG AGAATATTAG CGCCAAAATG AAGGGTCTCC GAGGGAAGGT GGCAGAACTA
    GAGAAGGCGG CTGCACAGCG CAAGGCCAAA CTTGATGAGA ATTCAGCTTT CCTGCAGTTC
    AACTGGAAGG CGGATGTTGT GGAGTCCTGG ATCGGTGAAA AAGAGAACAG CCTGAAGACT
    GATGATTATG GCAGAGACCT GTCCTCTGTG CAGACCCTTC TCACTAAGCA GGAAACATTT
    GATGCTGGGC TGCAAGCTTT CCAGCAAGAG GGCATTACCA ACATCACTGC ACTGAAAGAC
    CAGCTTCTGG CTGCAAAGCA TGTGCAGTCC CGGGCCATAG AGTCTCGTCA CGCTTCTCTG
    ATGAAGCGGT GGAACCAGTT GTTGGATAAC TCTGCTGCCC GCAAAAAGAA GTTATTGGAG
    GCCCAGGAAC ACTACAGAAA GGTTGAGGAC TTGTTCCTAA CATTTGCCAA GAAAGCCTCT
    GCCTTCAACA GCTGGTTTGA GAATGCCGAG GAGGATTTGA CTGATCCTGT TCGCTGCAAC
    TCCCTGGAGG AAATCAAGGC GCTTCGCGAG GCACATGACG CCTTCCGCAA TTCCCTCAGC
    TCCGCACAGA CAGATTTCAA CCAGCTGGCC GAACTGGACA GGCAAATAAA GGGCTACCGG
    GTAGCCTCCA ATCCCTACAC GTGGTTCACG ATGGAGGCTT TGGAGGAGAC ATGGCGCAAC
    CTGCAGAAAA TCATTAAGGA ACGAGAACTT GAGCTACAAA AAGAACAACG TAGGCAGGAA
    GAGAACGATA AGCTCCGGCA AGAGTTTGCT CAGCACGCCA ATGCTTTCCA CCAGTGGATT
    CAGGAGACAA GGACCTACCT TCTGGACGGG TCCTGTATGG TGGAAGAATC TGGAACACTG
    GAGTCACAAC TTGAAGCTAC AAAGAGAAAG CATCAGGAGA TCCGGGCCAT GAGGAGTCAA
    CTGAAGAAGA TAGAAGATCT TGGCGCAGCC ATGGAAGAGG CCCTGATCTT GGATAATAAA
    TACACAGAAC ACAGCACTGT TGGGCTGGCA CAGCAATGGG ACCAACTGGA TCAGCTAGGC
    ATGAGAATGC AACACAACCT AGAGCAACAG ATCCAGGCCA GAAACACAAC TGGGGTCACA
    GAGGAAGCAC TCAAAGAATT CAGCATGATG TTCAAGCATT TCGACAAAGA CAAGTCTGGC
    CGCCTCAACC ACCAAGAATT CAAATCTTGT CTGCGGTCCC TTGGCTATGA TCTGCCCATG
    GTAGAAGAAG GAGAACCAGA CCCAGAGTTT GAAGCCATTT TGGACACTGT AGATCCCAAC
    AGAGATGGGC ACGTTTCTCT ACAGGAGTAC ATGGCTTTCA TGATTAGCAG AGAAACTGAA
    AACGTCAAGT CCAGTGAGGA GATAGAGAGC GCCTTCCGAG CACTGAGTAC AGACCAGAAA
    CCTTATGTCA CAAAGGAAGA ACTATACCAG AACCTGACAC GCGAACAGGC AGATTACTGC
    ATCTCGCATA TGAAACCCTA CATGGACAGC AAGGGCCGCG AACTGCCCTC TGCATACGAC
    TACGTGGAAT TCACGCGCTC GCTATTTGTG AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031762668.1
    CDS4..7359
    Translation

    Target ORF information:

    RefSeq Version XM_031906808.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis spectrin, alpha, non-erythrocytic 1 (sptan1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031906808.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGGATTCAG GTGGCGTGAA AGTGCTGGAG ACAGCGGAGG ACATCCAGGA AAGGCGCCAG 
    CAAGTCCTAG ACCGATACCT GCGGTTTAAG GAACTCTCTG TACTGAGACG TCAGAAGTTG
    GAGGATTCTT ACCGCTTCCA GTTCTTCCAG CGAGATGCTG ATGAACTGGA GAAATGGATC
    CAGGAGAAAC TCCAGATCGC ATCTGATGAG AACTACAAAG ATCCAACCAA TCTGCAGGGC
    AAATTGCAGA AACACCAGGC TTTTGAGGCA GAAGTACAGG CCAATGCAGG GGCAATTGTC
    AAACTGGACG AAACTGGAAA CCTGATGATT TCAGAAGGAC ATTTTTCATC AGAGACCATC
    AGAACCCGCC TGGTAGAGTT GCATCGTCTG TGGGAGCTGC TGCTCTTAAA GATGCAAGAG
    AAAGGGGTTA AATTACAACA GGCGCAGAAG CTTGTTCAGT ATCTACGGGA ATGTGAAGAT
    GTCCTAGACT GGATTAATGA CAAGGAGGCT ATTGTAACAT CTGAGGAGCT TGGTCAGGAT
    TTGGAACATG TGGAGGTCTT ACAGAAGAAG TTTGAAGAGT TTCAGACTGA TCTGGCAGCT
    CATGAAGAAA GAGTGAATGA AGTCAACCAG TTTGCAAGTA AACTGGCCCA GGAGGAACAC
    CCGGAGCTGG AGCTAATAAA GACCAAGCAG GATGAAGTCA ATGCAAGTTG GCAGCGCCTC
    AAAGGGCTGG CACTTCAGAG ACAAGGGAAG TTATTTGGAG CTGCAGAAGT TCAGCGTTTT
    AACAGAGATG TTGATGAAAC CATTGGATGG ATAAGAGAGA AGGAGCAGCT GATGGCATCT
    GATGACTTTG GCCGAGACCT GGCTAGCGTT CAAGCTCTCC TACGTAAACA CGAGGGTCTG
    GAGAGAGACC TTGCAGCACT GGAGGAAAAG GTAAAGGCCT TGTGTGCAGA ATCAGACCGT
    CTGCAGCAAT CCCATCCCAT GAACGCCCCT CAAATTCAAG TGAAAGGAGA AGAACTGATA
    ACCAACTGGG AGCAAATACG CACATTGGCA GCAGAACGAC ATGCTCGACT CAATGACTCT
    TACAAGCTTC AGCGTTTCCT TGCAGATTTT AGAGATCTGA CTAGCTGGGT GACTGAAATG
    AAAGCTCTGA TTAATGCCGA TGAGCTTGCC AACGATGTGG CTGGCGCAGA GGCTCTGCTT
    GACCGACACC AGGAACATAA GGGGGAAATT GATGCTCATG AAGATAGCTT TAAAGCTGCA
    GATGCTGCAG GTCAGGCCCT TCTGAACGGT GGCCACTACG CCTCAGATGA AGTCCGAGAA
    AAGCTTACTA TCTTAGCAGA AGAAAGGGCA GCTCTTCTAG AGCTTTGGGA GCTCCGTCGT
    CAGCAGTATG AACAGTGCAT GGACCTGCAG CTTTTTTACA GAGACACCGA ACAGGTGGAC
    AACTGGATGA GCAAACAGGA AGCTTTCCTG CTTAACGAGG ACTTGGGTGA TTCTCTAGAC
    AGTGTGGAAG CTCTTCTGAA AAAACATGAG GACTTTGAGA AGTCTCTTAG CGCCCAGGAA
    GAGAAAATCA CAGCTCTGGA TGAGTTTGCT ACAAAACTAA TTCAAAATAA CCACTATGCC
    AAGGAAGATG TGGCTACCCG CAGAGATGCT CTGCTGAGCC GCCGCAATGC ACTTCATGAA
    CGTGCACTCT ACCGCCGTAC TCAACTGGCA GACTCCTTTC ACCTGCAGCA GTTCTTTAGG
    GACTCTGACG AGCTGAAAAG TTGGATTAAT GAAAAGATGA AGACTGCAAC GGATGAGGCA
    TACAAGGATC CCTCCAACCT CCAGGGTAAG GTGCAGAAAC ACCAAGCTTT TGAGGCTGAA
    CTCTCCGCTA ACCAAAGCCG TATAGATGCC CTGGAGAATT CTGGGCAGAA ATTGATTGAT
    GTCAACCATT ATGCATCAGA TGAGGTGGCA GCACGTATGA GTGAGGTCAT CACTTTGTGG
    AAGAAGCTGC TGGAGGCCAC TGAATTGAAG GGGATCAAGC TGCGCGAAGC CAACCAGCAG
    CAGCAGTTCA ACCGTAATGT GGAGGATATA GAGCTGTGGC TGTATGAGGT TGAGGGGCAT
    TTGGCTTCAG ATGACTATGG AAAAGACCTG ACCAATGTTC AAAACTTGCA GAAAAAACAC
    GCCTTGCTGG AGGCTGATGT TGCAGCTCAC CAGGACCGTA TCGATGGCAT CACTATCCAA
    GCCCGTCAGT TCCAAGAAGC TGGTCATTTT GATGCTGACA ATATCAGGAA GAAACAGGAG
    GCCCTTGTTA ATAGATATGA AGCTTTGAAA GAACCCATGG TTGCACGCAA GCAGAAGCTG
    TCAGACTCTC TGCGTCTTCA ACAGCTTTTC AGGGATGTTG AAGATGAGGA AACCTGGATA
    AGGGAGAAGG AACCCATTGC AGCGTCCACC AACAGAGGCA AGGATTTGAT TGGAGTCCAG
    AATCTCCTGA AGAAACACCA GGCTTTGCAG GCTGAAATTG CAGGGCACGA ACCACGAATT
    AAAGCTGTTA CCCAGAAAGG AGAATCTATG ATCTCTGAAG GCCACTTTGC TTCTGATGAA
    GTGAAGGGCA AATTAAGGGA GCTGAATGAT AAGTGGGCCA CCCTGAAGAA CAAAGCATCT
    CAGCGCCGAC AGGACCTTGA AGATTCTCTG CAAGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCAGAGT CCTGGATGAG AGAGAAGGAA CCTATTGTTG GAAGCACCGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC CCTTCTGAAG AAACATGAAG CGCTTATGTC AGATCTGCGA
    GCATACGGAA GCAGTATTCA GGGACTTAGA GAGCAGGCCC AGGCCTGCAG GCAACAAGTT
    GCTCCAACTG ATGATGAAAC TGGAAAGGAG CTGGTGCTGG CATTGTATGA TTATCAAGAG
    AAGAGCCCTA GGGAGGTGAC AATGAAGAAA GGAGACATCT TGACCTTGCT AAACAGCACA
    AATAAGGATT GGTGGAAGGT GGAAGTGAAT GATCGTCAGG GCTTTGTACC TGCTGCGTAT
    GTGAAAAAGC TGGACCCAGC ACAGTCCGCA TCCCGGGAAA ACTTGCTGGA GGAACAGGGA
    AGCATCGCTC TCCGTCAGGA GCAGATCGAT GGCCAGTATC ATAATATTTT GGAGATGGGA
    GAGAAGCGCA AAGATATGTT AGAAAAGAGC TGCAAGAAGT TTATGCTGTT CCGTGAGGCA
    AATGAGCTGC AGCAGTGGAT TAACGAGAAA GAAGCATCAC TCACTAACGA AGAGGTCGGG
    GCTGACCTGG AACAAGTGGA AGTGCTGCAG AAGAAGTTTG ATGATTTCCA GAAGGATCTG
    AAAGCAAATG AGTCACGCTT GAAGGACATC AACAAGGTGG CCGACGAGCT TGAATCTGAA
    GGCCTCATTA CAGAGGAAGT CCAGCCGGTG CAGCATCAGG AGGTGTATGG GATGCAGCGG
    GATGAAGCAG ACTCCAAATC GGCATCTCCA TGGAAGTCTG TTCGCACAGC TGTGCTCGCG
    GTGGCCACCT TCAACTCTAT CAAGGACTTG AACGAGCGCT GGAAATCACT TCAACAGCTG
    GCAGAAGAAA GGAGTCAGCT TCTTGGCAGT GCCCATGAAG TACAGAGATT CCACAGAGAT
    GCTGATGAGA CAAAAGAATG GATCGAAGAG AAGAACCAGG CTTTGAATAC AGACAGCTAT
    GGACATGACT TGGCCAGTGT GCAGGCTCTG CAACGAAAAC ATGAAGGCTT TGAGAGAGAC
    CTCGCCGCTC TGGGAGATAA GGTGAACTCT TTGGGAGAAA CTGCTGAGCG CCTGATCCAG
    TCACACCCTG AGTCTGCAGA TGACATTCAG GAAAAATGCA CAGAGCTGAA TCAGGCCTGG
    AACAGCCTTG GCACCCGGGC AAACCAGCGC AAGGAGAAAC TAGGCGATTC TCATGATCTG
    CAACGCTTCC TAAGTGACTT CAGAGATCTC ATGTCCTGGA TCAATGGAAT TCGGGGCTTG
    GTGTCATCAG ATGAACTTGC TAGAGATGTG ACTGGTGCAG AGGCTTTGCT GGAACGACAC
    CAGGAGCACA GGACTGAGAT TGATGCCAGA GCAGGCACTT TTCAGGCCTT TGAGCAGTTT
    GGGCAGCAAC TTCTGGCTCG TGGTCATTAT GATAGTCCTG AAATCAAGGA GAAGCTGGAT
    ATCTTGGAAC AGGAACGGGC AAGTTTGGAA AAGGCTTGGG TGGAACGCAG AATGATGTTG
    GATCAGTGCT TGGAGCTGCA GCTCTTCAAC AGAGACTGCG AGCAGGCAGA GAACTGGATG
    GCAGCCCGTG AAGCCTTCCT GAACAGTGAG GATAAAGGGG ATTCCCTGGA CAGTGTGGAG
    GCCCTTATCA AGAAACATGA AGACTTTGAT AAAGCCATTA ATGTCCAGGA AGAGAAGATT
    GCTGCCCTCC AGGCCTTCGC CGAACAATTG ATAATTGCTG ACCACTATGC CAAAGGAGAT
    ATTGCCTCCC GTCGCAATGA AGTCCTGGAC AGGTGGCGCC GTCTCAAGGC TCAGATGATT
    GAGAAGAGAT CCAAACTTGG GGAATCTCAG ACTCTGCAGC AGTTCAGCAG GGACGTCGAT
    GAGATCGAAG CCTGGATCAG CGAGAAATTG CAGACCGCCA GTGACGAGTC CTACAAAGAC
    CCCACAAACA TCCAGAGCAA ACACCAGAAG CACCAGGCTT TCGAAGCTGA GCTCCATGCC
    AACGCTGACC GTATTCGTGG AGTAGTAGAT ATGGGGAACT CCCTTATCGA ACGAGGTGCC
    TGTGCTGGCA GCGAGGATGC GGTGAAGGCT CGCCTAAGCG CACTCGCTGA TCAATGGCAG
    TTCCTTGTCA GCAAATCCGC AGAGAAGAGC CAGAAACTGA AGGAGGCAAA CAAGCAGCAG
    AACTTTAACA CTGGCATCAA AGACTTTGAT TTCTGGTTAA CAGAGGTGGA GGCTTTGCTG
    GCATCTGAAG ACTATGGGAA GGACTTGGCA TCTGTTAACA ACCTGCTGAA GAAACACCAG
    CTTCTGGAAG CTGACATCTC TGCTCATGAG GACCGCCTTA AAGATCTTAA TGGGCAGGCT
    GATAGCCTGA TGACCAGCAG CGCCTTTGAC ACCACCCAAG TAAAGGAGAA AAGGGACGCC
    ATCAACGACC GCTTCCAGCG TATCAAAAGC ATGGCCACCG CACGCCGCAG CAGGCTGAAT
    GAGTCCCACC GTCTGCACCA GTTCTTCAGA GATCTTGATG ATGAGGAATC ATGGATCAAG
    GAAAAGAAAC TTTTGGTCGG ATCTGATGAC TATGGACGTG ACTTGACTGG AGTGCAAAAT
    CTGAGGAAGA AACACAAGAG ATTAGAAGCT GAACTGGCAG CTCATGAGCC AGCCATTCAG
    GGTGTGCTGG ACACTGGGAA GAAGCTTGCA GATGACAACA CTATTGGGAA AGATGAGATT
    CAGCAGCGAC TGGCACAGTT TCTAGAGCAC TGGAAGGAGC TGAAGGAACA AGCAGCGGCT
    CGTGGTCAGA GACTGGAGGA ATCTCTTGAG TACCAACAAT TTGTAGCCAA TGTGGAGGAA
    GAGGAAGCCT GGATTAATGA GAAGATGACT CTAGTGGCCA GTGAAGACTA TGGTGACACA
    CTGGCTGCCA TTCAGGGATT GCTGAAAAAG CACGAGGCAT TTGAGACAGA CTTTACAGTG
    CACAAAGACA GAGTACATGA TGTATGTGCC AACGGGGAAG ACCTGATACA AAAGAACAAT
    CACCATGTGG AGAATATTAG CGCCAAAATG AAGGGTCTCC GAGGGAAGGT GGCAGAACTA
    GAGAAGGCGG CTGCACAGCG CAAGGCCAAA CTTGATGAGA ATTCAGCTTT CCTGCAGTTC
    AACTGGAAGG CGGATGTTGT GGAGTCCTGG ATCGGTGAAA AAGAGAACAG CCTGAAGACT
    GATGATTATG GCAGAGACCT GTCCTCTGTG CAGACCCTTC TCACTAAGCA GGAAACATTT
    GATGCTGGGC TGCAAGCTTT CCAGCAAGAG GGCATTACCA ACATCACTGC ACTGAAAGAC
    CAGCTTCTGG CTGCAAAGCA TGTGCAGTCC CGGGCCATAG AGTCTCGTCA CGCTTCTCTG
    ATGAAGCGGT GGAACCAGTT GTTGGATAAC TCTGCTGCCC GCAAAAAGAA GTTATTGGAG
    GCCCAGGAAC ACTACAGAAA GGTTGAGGAC TTGTTCCTAA CATTTGCCAA GAAAGCCTCT
    GCCTTCAACA GCTGGTTTGA GAATGCCGAG GAGGATTTGA CTGATCCTGT TCGCTGCAAC
    TCCCTGGAGG AAATCAAGGC GCTTCGCGAG GCACATGACG CCTTCCGCAA TTCCCTCAGC
    TCCGCACAGA CAGATTTCAA CCAGCTGGCC GAACTGGACA GGCAAATAAA GGGCTACCGG
    GTAGCCTCCA ATCCCTACAC GTGGTTCACG ATGGAGGCTT TGGAGGAGAC ATGGCGCAAC
    CTGCAGAAAA TCATTAAGGA ACGAGAACTT GAGCTACAAA AAGAACAACG TAGGCAGGAA
    GAGAACGATA AGCTCCGGCA AGAGTTTGCT CAGCACGCCA ATGCTTTCCA CCAGTGGATT
    CAGGAGACAA GGACCTACCT TCTGGACGGG TCCTGTATGG TGGAAGAATC TGGAACACTG
    GAGTCACAAC TTGAAGCTAC AAAGAGAAAG CATCAGGAGA TCCGGGCCAT GAGGAGTCAA
    CTGAAGAAGA TAGAAGATCT TGGCGCAGCC ATGGAAGAGG CCCTGATCTT GGATAATAAA
    TACACAGAAC ACAGCACTGT TGGGCTGGCA CAGCAATGGG ACCAACTGGA TCAGCTAGGC
    ATGAGAATGC AACACAACCT AGAGCAACAG ATCCAGGCCA GAAACACAAC TGGGGTCACA
    GAGGAAGCAC TCAAAGAATT CAGCATGATG TTCAAGCATT TCGACAAAGA CAAGTCTGGC
    CGCCTCAACC ACCAAGAATT CAAATCTTGT CTGCGGTCCC TTGGCTATGA TCTGCCCATG
    GTAGAAGAAG GAGAACCAGA CCCAGAGTTT GAAGCCATTT TGGACACTGT AGATCCCAAC
    AGAGATGGGC ACGTTTCTCT ACAGGAGTAC ATGGCTTTCA TGATTAGCAG AGAAACTGAA
    AACGTCAAGT CCAGTGAGGA GATAGAGAGC GCCTTCCGAG CACTGAGTAC AGACCAGAAA
    CCTTATGTCA CAAAGGAAGA ACTATACCAG AACCTGACAC GCGAACAGGC AGATTACTGC
    ATCTCGCATA TGAAACCCTA CATGGACAGC AAGGGCCGCG AACTGCCCTC TGCATACGAC
    TACGTGGAAT TCACGCGCTC GCTATTTGTG AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa63342
    Clone ID Related Accession (Same CDS sequence) XM_031906802.1
    Accession Version XM_031906802.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7494bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGGATTCAG GTGGCGTGAA AGTGCTGGAG ACAGCGGAGG ACATCCAGGA AAGGCGCCAG 
    CAAGTCCTAG ACCGATACCT GCGGTTTAAG GAACTCTCTG TACTGAGACG TCAGAAGTTG
    GAGGATTCTT ACCGCTTCCA GTTCTTCCAG CGAGATGCTG ATGAACTGGA GAAATGGATC
    CAGGAGAAAC TCCAGATCGC ATCTGATGAG AACTACAAAG ATCCAACCAA TCTGCAGGGC
    AAATTGCAGA AACACCAGGC TTTTGAGGCA GAAGTACAGG CCAATGCAGG GGCAATTGTC
    AAACTGGACG AAACTGGAAA CCTGATGATT TCAGAAGGAC ATTTTTCATC AGAGACCATC
    AGAACCCGCC TGGTAGAGTT GCATCGTCTG TGGGAGCTGC TGCTCTTAAA GATGCAAGAG
    AAAGGGGTTA AATTACAACA GGCGCAGAAG CTTGTTCAGT ATCTACGGGA ATGTGAAGAT
    GTCCTAGACT GGATTAATGA CAAGGAGGCT ATTGTAACAT CTGAGGAGCT TGGTCAGGAT
    TTGGAACATG TGGAGGTCTT ACAGAAGAAG TTTGAAGAGT TTCAGACTGA TCTGGCAGCT
    CATGAAGAAA GAGTGAATGA AGTCAACCAG TTTGCAAGTA AACTGGCCCA GGAGGAACAC
    CCGGAGCTGG AGCTAATAAA GACCAAGCAG GATGAAGTCA ATGCAAGTTG GCAGCGCCTC
    AAAGGGCTGG CACTTCAGAG ACAAGGGAAG TTATTTGGAG CTGCAGAAGT TCAGCGTTTT
    AACAGAGATG TTGATGAAAC CATTGGATGG ATAAGAGAGA AGGAGCAGCT GATGGCATCT
    GATGACTTTG GCCGAGACCT GGCTAGCGTT CAAGCTCTCC TACGTAAACA CGAGGGTCTG
    GAGAGAGACC TTGCAGCACT GGAGGAAAAG GTAAAGGCCT TGTGTGCAGA ATCAGACCGT
    CTGCAGCAAT CCCATCCCAT GAACGCCCCT CAAATTCAAG TGAAAGGAGA AGAACTGATA
    ACCAACTGGG AGCAAATACG CACATTGGCA GCAGAACGAC ATGCTCGACT CAATGACTCT
    TACAAGCTTC AGCGTTTCCT TGCAGATTTT AGAGATCTGA CTAGCTGGGT GACTGAAATG
    AAAGCTCTGA TTAATGCCGA TGAGCTTGCC AACGATGTGG CTGGCGCAGA GGCTCTGCTT
    GACCGACACC AGGAACATAA GGGGGAAATT GATGCTCATG AAGATAGCTT TAAAGCTGCA
    GATGCTGCAG GTCAGGCCCT TCTGAACGGT GGCCACTACG CCTCAGATGA AGTCCGAGAA
    AAGCTTACTA TCTTAGCAGA AGAAAGGGCA GCTCTTCTAG AGCTTTGGGA GCTCCGTCGT
    CAGCAGTATG AACAGTGCAT GGACCTGCAG CTTTTTTACA GAGACACCGA ACAGGTGGAC
    AACTGGATGA GCAAACAGGA AGCTTTCCTG CTTAACGAGG ACTTGGGTGA TTCTCTAGAC
    AGTGTGGAAG CTCTTCTGAA AAAACATGAG GACTTTGAGA AGTCTCTTAG CGCCCAGGAA
    GAGAAAATCA CAGCTCTGGA TGAGTTTGCT ACAAAACTAA TTCAAAATAA CCACTATGCC
    AAGGAAGATG TGGCTACCCG CAGAGATGCT CTGCTGAGCC GCCGCAATGC ACTTCATGAA
    CGTGCACTCT ACCGCCGTAC TCAACTGGCA GACTCCTTTC ACCTGCAGCA GTTCTTTAGG
    GACTCTGACG AGCTGAAAAG TTGGATTAAT GAAAAGATGA AGACTGCAAC GGATGAGGCA
    TACAAGGATC CCTCCAACCT CCAGGGTAAG GTGCAGAAAC ACCAAGCTTT TGAGGCTGAA
    CTCTCCGCTA ACCAAAGCCG TATAGATGCC CTGGAGAATT CTGGGCAGAA ATTGATTGAT
    GTCAACCATT ATGCATCAGA TGAGGTGGCA GCACGTATGA GTGAGGTCAT CACTTTGTGG
    AAGAAGCTGC TGGAGGCCAC TGAATTGAAG GGGATCAAGC TGCGCGAAGC CAACCAGCAG
    CAGCAGTTCA ACCGTAATGT GGAGGATATA GAGCTGTGGC TGTATGAGGT TGAGGGGCAT
    TTGGCTTCAG ATGACTATGG AAAAGACCTG ACCAATGTTC AAAACTTGCA GAAAAAACAC
    GCCTTGCTGG AGGCTGATGT TGCAGCTCAC CAGGACCGTA TCGATGGCAT CACTATCCAA
    GCCCGTCAGT TCCAAGAAGC TGGTCATTTT GATGCTGACA ATATCAGGAA GAAACAGGAG
    GCCCTTGTTA ATAGATATGA AGCTTTGAAA GAACCCATGG TTGCACGCAA GCAGAAGCTG
    TCAGACTCTC TGCGTCTTCA ACAGCTTTTC AGGGATGTTG AAGATGAGGA AACCTGGATA
    AGGGAGAAGG AACCCATTGC AGCGTCCACC AACAGAGGCA AGGATTTGAT TGGAGTCCAG
    AATCTCCTGA AGAAACACCA GGCTTTGCAG GCTGAAATTG CAGGGCACGA ACCACGAATT
    AAAGCTGTTA CCCAGAAAGG AGAATCTATG ATCTCTGAAG GCCACTTTGC TTCTGATGAA
    GTGAAGGGCA AATTAAGGGA GCTGAATGAT AAGTGGGCCA CCCTGAAGAA CAAAGCATCT
    CAGCGCCGAC AGGACCTTGA AGATTCTCTG CAAGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCAGAGT CCTGGATGAG AGAGAAGGAA CCTATTGTTG GAAGCACCGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC CCTTCTGAAG AAACATGAAG CGCTTATGTC AGATCTGCGA
    GCATACGGAA GCAGTATTCA GGGACTTAGA GAGCAGGCCC AGGCCTGCAG GCAACAAGTT
    GCTCCAACTG ATGATGAAAC TGGAAAGGAG CTGGTGCTGG CATTGTATGA TTATCAAGAG
    AAGAGCCCTA GGGAGGTGAC AATGAAGAAA GGAGACATCT TGACCTTGCT AAACAGCACA
    AATAAGGATT GGTGGAAGGT GGAAGTGAAT GATCGTCAGG GCTTTGTACC TGCTGCGTAT
    GTGAAAAAGC TGGACCCAGC ACAGTCCGCA TCCCGGGAAA ACTTGCTGGA GGAACAGGGA
    AGCATCGCTC TCCGTCAGGA GCAGATCGAT GGCCAGGCTC ACATGTCTAA GGAAGCTGGC
    AGTGTTTCTC TGCGTATGAA TCAGGTTGAA GAATTGTATC ATAATATTTT GGAGATGGGA
    GAGAAGCGCA AAGATATGTT AGAAAAGAGC TGCAAGAAGT TTATGCTGTT CCGTGAGGCA
    AATGAGCTGC AGCAGTGGAT TAACGAGAAA GAAGCATCAC TCACTAACGA AGAGGTCGGG
    GCTGACCTGG AACAAGTGGA AGTGCTGCAG AAGAAGTTTG ATGATTTCCA GAAGGATCTG
    AAAGCAAATG AGTCACGCTT GAAGGACATC AACAAGGTGG CCGACGAGCT TGAATCTGAA
    GGCCTCATTA CAGAGGAAGT CCAGCCGGTG CAGCATCAGG AGGTGTATGG GATGCAGCGG
    GATGAAGCAG ACTCCAAATC GGCATCTCCA TGGAAGTCTG TTCGCACAGC TGTGCTCGCG
    GTGGCCACCT TCAACTCTAT CAAGGACTTG AACGAGCGCT GGAAATCACT TCAACAGCTG
    GCAGAAGAAA GGAGTCAGCT TCTTGGCAGT GCCCATGAAG TACAGAGATT CCACAGAGAT
    GCTGATGAGA CAAAAGAATG GATCGAAGAG AAGAACCAGG CTTTGAATAC AGACAGCTAT
    GGACATGACT TGGCCAGTGT GCAGGCTCTG CAACGAAAAC ATGAAGGCTT TGAGAGAGAC
    CTCGCCGCTC TGGGAGATAA GGTGAACTCT TTGGGAGAAA CTGCTGAGCG CCTGATCCAG
    TCACACCCTG AGTCTGCAGA TGACATTCAG GAAAAATGCA CAGAGCTGAA TCAGGCCTGG
    AACAGCCTTG GCACCCGGGC AAACCAGCGC AAGGAGAAAC TAGGCGATTC TCATGATCTG
    CAACGCTTCC TAAGTGACTT CAGAGATCTC ATGTCCTGGA TCAATGGAAT TCGGGGCTTG
    GTGTCATCAG ATGAACTTGC TAGAGATGTG ACTGGTGCAG AGGCTTTGCT GGAACGACAC
    CAGGAGCACA GGACTGAGAT TGATGCCAGA GCAGGCACTT TTCAGGCCTT TGAGCAGTTT
    GGGCAGCAAC TTCTGGCTCG TGGTCATTAT GATAGTCCTG AAATCAAGGA GAAGCTGGAT
    ATCTTGGAAC AGGAACGGGC AAGTTTGGAA AAGGCTTGGG TGGAACGCAG AATGATGTTG
    GATCAGTGCT TGGAGCTGCA GCTCTTCAAC AGAGACTGCG AGCAGGCAGA GAACTGGATG
    GCAGCCCGTG AAGCCTTCCT GAACAGTGAG GATAAAGGGG ATTCCCTGGA CAGTGTGGAG
    GCCCTTATCA AGAAACATGA AGACTTTGAT AAAGCCATTA ATGTCCAGGA AGAGAAGATT
    GCTGCCCTCC AGGCCTTCGC CGAACAATTG ATAATTGCTG ACCACTATGC CAAAGGAGAT
    ATTGCCTCCC GTCGCAATGA AGTCCTGGAC AGGTGGCGCC GTCTCAAGGC TCAGATGATT
    GAGAAGAGAT CCAAACTTGG GGAATCTCAG ACTCTGCAGC AGTTCAGCAG GGACGTCGAT
    GAGATCGAAG CCTGGATCAG CGAGAAATTG CAGACCGCCA GTGACGAGTC CTACAAAGAC
    CCCACAAACA TCCAGCTTTC CAAATTGCTG AGCAAACACC AGAAGCACCA GGCTTTCGAA
    GCTGAGCTCC ATGCCAACGC TGACCGTATT CGTGGAGTAG TAGATATGGG GAACTCCCTT
    ATCGAACGAG GTGCCTGTGC TGGCAGCGAG GATGCGGTGA AGGCTCGCCT AAGCGCACTC
    GCTGATCAAT GGCAGTTCCT TGTCAGCAAA TCCGCAGAGA AGAGCCAGAA ACTGAAGGAG
    GCAAACAAGC AGCAGAACTT TAACACTGGC ATCAAAGACT TTGATTTCTG GTTAACAGAG
    GTGGAGGCTT TGCTGGCATC TGAAGACTAT GGGAAGGACT TGGCATCTGT TAACAACCTG
    CTGAAGAAAC ACCAGCTTCT GGAAGCTGAC ATCTCTGCTC ATGAGGACCG CCTTAAAGAT
    CTTAATGGGC AGGCTGATAG CCTGATGACC AGCAGCGCCT TTGACACCAC CCAAGTAAAG
    GAGAAAAGGG ACGCCATCAA CGACCGCTTC CAGCGTATCA AAAGCATGGC CACCGCACGC
    CGCAGCAGGC TGAATGAGTC CCACCGTCTG CACCAGTTCT TCAGAGATCT TGATGATGAG
    GAATCATGGA TCAAGGAAAA GAAACTTTTG GTCGGATCTG ATGACTATGG ACGTGACTTG
    ACTGGAGTGC AAAATCTGAG GAAGAAACAC AAGAGATTAG AAGCTGAACT GGCAGCTCAT
    GAGCCAGCCA TTCAGGGTGT GCTGGACACT GGGAAGAAGC TTGCAGATGA CAACACTATT
    GGGAAAGATG AGATTCAGCA GCGACTGGCA CAGTTTCTAG AGCACTGGAA GGAGCTGAAG
    GAACAAGCAG CGGCTCGTGG TCAGAGACTG GAGGAATCTC TTGAGTACCA ACAATTTGTA
    GCCAATGTGG AGGAAGAGGA AGCCTGGATT AATGAGAAGA TGACTCTAGT GGCCAGTGAA
    GACTATGGTG ACACACTGGC TGCCATTCAG GGATTGCTGA AAAAGCACGA GGCATTTGAG
    ACAGACTTTA CAGTGCACAA AGACAGAGTA CATGATGTAT GTGCCAACGG GGAAGACCTG
    ATACAAAAGA ACAATCACCA TGTGGAGAAT ATTAGCGCCA AAATGAAGGG TCTCCGAGGG
    AAGGTGGCAG AACTAGAGAA GGCGGCTGCA CAGCGCAAGG CCAAACTTGA TGAGAATTCA
    GCTTTCCTGC AGTTCAACTG GAAGGCGGAT GTTGTGGAGT CCTGGATCGG TGAAAAAGAG
    AACAGCCTGA AGACTGATGA TTATGGCAGA GACCTGTCCT CTGTGCAGAC CCTTCTCACT
    AAGCAGGAAA CATTTGATGC TGGGCTGCAA GCTTTCCAGC AAGAGGGCAT TACCAACATC
    ACTGCACTGA AAGACCAGCT TCTGGCTGCA AAGCATGTGC AGTCCCGGGC CATAGAGTCT
    CGTCACGCTT CTCTGATGAA GCGGTGGAAC CAGTTGTTGG ATAACTCTGC TGCCCGCAAA
    AAGAAGTTAT TGGAGGCCCA GGAACACTAC AGAAAGGTTG AGGACTTGTT CCTAACATTT
    GCCAAGAAAG CCTCTGCCTT CAACAGCTGG TTTGAGAATG CCGAGGAGGA TTTGACTGAT
    CCTGTTCGCT GCAACTCCCT GGAGGAAATC AAGGCGCTTC GCGAGGCACA TGACGCCTTC
    CGCAATTCCC TCAGCTCCGC ACAGACAGAT TTCAACCAGC TGGCCGAACT GGACAGGCAA
    ATAAAGGGCT ACCGGGTAGC CTCCAATCCC TACACGTGGT TCACGATGGA GGCTTTGGAG
    GAGACATGGC GCAACCTGCA GAAAATCATT AAGGAACGAG AACTTGAGCT ACAAAAAGAA
    CAACGTAGGC AGGAAGAGAA CGATAAGCTC CGGCAAGAGT TTGCTCAGCA CGCCAATGCT
    TTCCACCAGT GGATTCAGGA GACAAGGACC TACCTTCTGG ACGGAATAGC CTATCGCAGA
    GTCATACGTG TGTATCAGTA TGAAGTTGGG GATGATCTTT CTGGAAGGTC CTGTATGGTG
    GAAGAATCTG GAACACTGGA GTCACAACTT GAAGCTACAA AGAGAAAGCA TCAGGAGATC
    CGGGCCATGA GGAGTCAACT GAAGAAGATA GAAGATCTTG GCGCAGCCAT GGAAGAGGCC
    CTGATCTTGG ATAATAAATA CACAGAACAC AGCACTGTTG GGCTGGCACA GCAATGGGAC
    CAACTGGATC AGCTAGGCAT GAGAATGCAA CACAACCTAG AGCAACAGAT CCAGGCCAGA
    AACACAACTG GGGTCACAGA GGAAGCACTC AAAGAATTCA GCATGATGTT CAAGCATTTC
    GACAAAGACA AGTCTGGCCG CCTCAACCAC CAAGAATTCA AATCTTGTCT GCGGTCCCTT
    GGCTATGATC TGCCCATGGT AGAAGAAGGA GAACCAGACC CAGAGTTTGA AGCCATTTTG
    GACACTGTAG ATCCCAACAG AGATGGGCAC GTTTCTCTAC AGGAGTACAT GGCTTTCATG
    ATTAGCAGAG AAACTGAAAA CGTCAAGTCC AGTGAGGAGA TAGAGAGCGC CTTCCGAGCA
    CTGAGTACAG ACCAGAAACC TTATGTCACA AAGGAAGAAC TATACCAGAA CCTGACACGC
    GAACAGGCAG ATTACTGCAT CTCGCATATG AAACCCTACA TGGACAGCAA GGGCCGCGAA
    CTGCCCTCTG CATACGACTA CGTGGAATTC ACGCGCTCGC TATTTGTGAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031762662.1
    CDS4..7497
    Translation

    Target ORF information:

    RefSeq Version XM_031906802.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis spectrin, alpha, non-erythrocytic 1 (sptan1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031906802.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGGATTCAG GTGGCGTGAA AGTGCTGGAG ACAGCGGAGG ACATCCAGGA AAGGCGCCAG 
    CAAGTCCTAG ACCGATACCT GCGGTTTAAG GAACTCTCTG TACTGAGACG TCAGAAGTTG
    GAGGATTCTT ACCGCTTCCA GTTCTTCCAG CGAGATGCTG ATGAACTGGA GAAATGGATC
    CAGGAGAAAC TCCAGATCGC ATCTGATGAG AACTACAAAG ATCCAACCAA TCTGCAGGGC
    AAATTGCAGA AACACCAGGC TTTTGAGGCA GAAGTACAGG CCAATGCAGG GGCAATTGTC
    AAACTGGACG AAACTGGAAA CCTGATGATT TCAGAAGGAC ATTTTTCATC AGAGACCATC
    AGAACCCGCC TGGTAGAGTT GCATCGTCTG TGGGAGCTGC TGCTCTTAAA GATGCAAGAG
    AAAGGGGTTA AATTACAACA GGCGCAGAAG CTTGTTCAGT ATCTACGGGA ATGTGAAGAT
    GTCCTAGACT GGATTAATGA CAAGGAGGCT ATTGTAACAT CTGAGGAGCT TGGTCAGGAT
    TTGGAACATG TGGAGGTCTT ACAGAAGAAG TTTGAAGAGT TTCAGACTGA TCTGGCAGCT
    CATGAAGAAA GAGTGAATGA AGTCAACCAG TTTGCAAGTA AACTGGCCCA GGAGGAACAC
    CCGGAGCTGG AGCTAATAAA GACCAAGCAG GATGAAGTCA ATGCAAGTTG GCAGCGCCTC
    AAAGGGCTGG CACTTCAGAG ACAAGGGAAG TTATTTGGAG CTGCAGAAGT TCAGCGTTTT
    AACAGAGATG TTGATGAAAC CATTGGATGG ATAAGAGAGA AGGAGCAGCT GATGGCATCT
    GATGACTTTG GCCGAGACCT GGCTAGCGTT CAAGCTCTCC TACGTAAACA CGAGGGTCTG
    GAGAGAGACC TTGCAGCACT GGAGGAAAAG GTAAAGGCCT TGTGTGCAGA ATCAGACCGT
    CTGCAGCAAT CCCATCCCAT GAACGCCCCT CAAATTCAAG TGAAAGGAGA AGAACTGATA
    ACCAACTGGG AGCAAATACG CACATTGGCA GCAGAACGAC ATGCTCGACT CAATGACTCT
    TACAAGCTTC AGCGTTTCCT TGCAGATTTT AGAGATCTGA CTAGCTGGGT GACTGAAATG
    AAAGCTCTGA TTAATGCCGA TGAGCTTGCC AACGATGTGG CTGGCGCAGA GGCTCTGCTT
    GACCGACACC AGGAACATAA GGGGGAAATT GATGCTCATG AAGATAGCTT TAAAGCTGCA
    GATGCTGCAG GTCAGGCCCT TCTGAACGGT GGCCACTACG CCTCAGATGA AGTCCGAGAA
    AAGCTTACTA TCTTAGCAGA AGAAAGGGCA GCTCTTCTAG AGCTTTGGGA GCTCCGTCGT
    CAGCAGTATG AACAGTGCAT GGACCTGCAG CTTTTTTACA GAGACACCGA ACAGGTGGAC
    AACTGGATGA GCAAACAGGA AGCTTTCCTG CTTAACGAGG ACTTGGGTGA TTCTCTAGAC
    AGTGTGGAAG CTCTTCTGAA AAAACATGAG GACTTTGAGA AGTCTCTTAG CGCCCAGGAA
    GAGAAAATCA CAGCTCTGGA TGAGTTTGCT ACAAAACTAA TTCAAAATAA CCACTATGCC
    AAGGAAGATG TGGCTACCCG CAGAGATGCT CTGCTGAGCC GCCGCAATGC ACTTCATGAA
    CGTGCACTCT ACCGCCGTAC TCAACTGGCA GACTCCTTTC ACCTGCAGCA GTTCTTTAGG
    GACTCTGACG AGCTGAAAAG TTGGATTAAT GAAAAGATGA AGACTGCAAC GGATGAGGCA
    TACAAGGATC CCTCCAACCT CCAGGGTAAG GTGCAGAAAC ACCAAGCTTT TGAGGCTGAA
    CTCTCCGCTA ACCAAAGCCG TATAGATGCC CTGGAGAATT CTGGGCAGAA ATTGATTGAT
    GTCAACCATT ATGCATCAGA TGAGGTGGCA GCACGTATGA GTGAGGTCAT CACTTTGTGG
    AAGAAGCTGC TGGAGGCCAC TGAATTGAAG GGGATCAAGC TGCGCGAAGC CAACCAGCAG
    CAGCAGTTCA ACCGTAATGT GGAGGATATA GAGCTGTGGC TGTATGAGGT TGAGGGGCAT
    TTGGCTTCAG ATGACTATGG AAAAGACCTG ACCAATGTTC AAAACTTGCA GAAAAAACAC
    GCCTTGCTGG AGGCTGATGT TGCAGCTCAC CAGGACCGTA TCGATGGCAT CACTATCCAA
    GCCCGTCAGT TCCAAGAAGC TGGTCATTTT GATGCTGACA ATATCAGGAA GAAACAGGAG
    GCCCTTGTTA ATAGATATGA AGCTTTGAAA GAACCCATGG TTGCACGCAA GCAGAAGCTG
    TCAGACTCTC TGCGTCTTCA ACAGCTTTTC AGGGATGTTG AAGATGAGGA AACCTGGATA
    AGGGAGAAGG AACCCATTGC AGCGTCCACC AACAGAGGCA AGGATTTGAT TGGAGTCCAG
    AATCTCCTGA AGAAACACCA GGCTTTGCAG GCTGAAATTG CAGGGCACGA ACCACGAATT
    AAAGCTGTTA CCCAGAAAGG AGAATCTATG ATCTCTGAAG GCCACTTTGC TTCTGATGAA
    GTGAAGGGCA AATTAAGGGA GCTGAATGAT AAGTGGGCCA CCCTGAAGAA CAAAGCATCT
    CAGCGCCGAC AGGACCTTGA AGATTCTCTG CAAGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCAGAGT CCTGGATGAG AGAGAAGGAA CCTATTGTTG GAAGCACCGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC CCTTCTGAAG AAACATGAAG CGCTTATGTC AGATCTGCGA
    GCATACGGAA GCAGTATTCA GGGACTTAGA GAGCAGGCCC AGGCCTGCAG GCAACAAGTT
    GCTCCAACTG ATGATGAAAC TGGAAAGGAG CTGGTGCTGG CATTGTATGA TTATCAAGAG
    AAGAGCCCTA GGGAGGTGAC AATGAAGAAA GGAGACATCT TGACCTTGCT AAACAGCACA
    AATAAGGATT GGTGGAAGGT GGAAGTGAAT GATCGTCAGG GCTTTGTACC TGCTGCGTAT
    GTGAAAAAGC TGGACCCAGC ACAGTCCGCA TCCCGGGAAA ACTTGCTGGA GGAACAGGGA
    AGCATCGCTC TCCGTCAGGA GCAGATCGAT GGCCAGGCTC ACATGTCTAA GGAAGCTGGC
    AGTGTTTCTC TGCGTATGAA TCAGGTTGAA GAATTGTATC ATAATATTTT GGAGATGGGA
    GAGAAGCGCA AAGATATGTT AGAAAAGAGC TGCAAGAAGT TTATGCTGTT CCGTGAGGCA
    AATGAGCTGC AGCAGTGGAT TAACGAGAAA GAAGCATCAC TCACTAACGA AGAGGTCGGG
    GCTGACCTGG AACAAGTGGA AGTGCTGCAG AAGAAGTTTG ATGATTTCCA GAAGGATCTG
    AAAGCAAATG AGTCACGCTT GAAGGACATC AACAAGGTGG CCGACGAGCT TGAATCTGAA
    GGCCTCATTA CAGAGGAAGT CCAGCCGGTG CAGCATCAGG AGGTGTATGG GATGCAGCGG
    GATGAAGCAG ACTCCAAATC GGCATCTCCA TGGAAGTCTG TTCGCACAGC TGTGCTCGCG
    GTGGCCACCT TCAACTCTAT CAAGGACTTG AACGAGCGCT GGAAATCACT TCAACAGCTG
    GCAGAAGAAA GGAGTCAGCT TCTTGGCAGT GCCCATGAAG TACAGAGATT CCACAGAGAT
    GCTGATGAGA CAAAAGAATG GATCGAAGAG AAGAACCAGG CTTTGAATAC AGACAGCTAT
    GGACATGACT TGGCCAGTGT GCAGGCTCTG CAACGAAAAC ATGAAGGCTT TGAGAGAGAC
    CTCGCCGCTC TGGGAGATAA GGTGAACTCT TTGGGAGAAA CTGCTGAGCG CCTGATCCAG
    TCACACCCTG AGTCTGCAGA TGACATTCAG GAAAAATGCA CAGAGCTGAA TCAGGCCTGG
    AACAGCCTTG GCACCCGGGC AAACCAGCGC AAGGAGAAAC TAGGCGATTC TCATGATCTG
    CAACGCTTCC TAAGTGACTT CAGAGATCTC ATGTCCTGGA TCAATGGAAT TCGGGGCTTG
    GTGTCATCAG ATGAACTTGC TAGAGATGTG ACTGGTGCAG AGGCTTTGCT GGAACGACAC
    CAGGAGCACA GGACTGAGAT TGATGCCAGA GCAGGCACTT TTCAGGCCTT TGAGCAGTTT
    GGGCAGCAAC TTCTGGCTCG TGGTCATTAT GATAGTCCTG AAATCAAGGA GAAGCTGGAT
    ATCTTGGAAC AGGAACGGGC AAGTTTGGAA AAGGCTTGGG TGGAACGCAG AATGATGTTG
    GATCAGTGCT TGGAGCTGCA GCTCTTCAAC AGAGACTGCG AGCAGGCAGA GAACTGGATG
    GCAGCCCGTG AAGCCTTCCT GAACAGTGAG GATAAAGGGG ATTCCCTGGA CAGTGTGGAG
    GCCCTTATCA AGAAACATGA AGACTTTGAT AAAGCCATTA ATGTCCAGGA AGAGAAGATT
    GCTGCCCTCC AGGCCTTCGC CGAACAATTG ATAATTGCTG ACCACTATGC CAAAGGAGAT
    ATTGCCTCCC GTCGCAATGA AGTCCTGGAC AGGTGGCGCC GTCTCAAGGC TCAGATGATT
    GAGAAGAGAT CCAAACTTGG GGAATCTCAG ACTCTGCAGC AGTTCAGCAG GGACGTCGAT
    GAGATCGAAG CCTGGATCAG CGAGAAATTG CAGACCGCCA GTGACGAGTC CTACAAAGAC
    CCCACAAACA TCCAGCTTTC CAAATTGCTG AGCAAACACC AGAAGCACCA GGCTTTCGAA
    GCTGAGCTCC ATGCCAACGC TGACCGTATT CGTGGAGTAG TAGATATGGG GAACTCCCTT
    ATCGAACGAG GTGCCTGTGC TGGCAGCGAG GATGCGGTGA AGGCTCGCCT AAGCGCACTC
    GCTGATCAAT GGCAGTTCCT TGTCAGCAAA TCCGCAGAGA AGAGCCAGAA ACTGAAGGAG
    GCAAACAAGC AGCAGAACTT TAACACTGGC ATCAAAGACT TTGATTTCTG GTTAACAGAG
    GTGGAGGCTT TGCTGGCATC TGAAGACTAT GGGAAGGACT TGGCATCTGT TAACAACCTG
    CTGAAGAAAC ACCAGCTTCT GGAAGCTGAC ATCTCTGCTC ATGAGGACCG CCTTAAAGAT
    CTTAATGGGC AGGCTGATAG CCTGATGACC AGCAGCGCCT TTGACACCAC CCAAGTAAAG
    GAGAAAAGGG ACGCCATCAA CGACCGCTTC CAGCGTATCA AAAGCATGGC CACCGCACGC
    CGCAGCAGGC TGAATGAGTC CCACCGTCTG CACCAGTTCT TCAGAGATCT TGATGATGAG
    GAATCATGGA TCAAGGAAAA GAAACTTTTG GTCGGATCTG ATGACTATGG ACGTGACTTG
    ACTGGAGTGC AAAATCTGAG GAAGAAACAC AAGAGATTAG AAGCTGAACT GGCAGCTCAT
    GAGCCAGCCA TTCAGGGTGT GCTGGACACT GGGAAGAAGC TTGCAGATGA CAACACTATT
    GGGAAAGATG AGATTCAGCA GCGACTGGCA CAGTTTCTAG AGCACTGGAA GGAGCTGAAG
    GAACAAGCAG CGGCTCGTGG TCAGAGACTG GAGGAATCTC TTGAGTACCA ACAATTTGTA
    GCCAATGTGG AGGAAGAGGA AGCCTGGATT AATGAGAAGA TGACTCTAGT GGCCAGTGAA
    GACTATGGTG ACACACTGGC TGCCATTCAG GGATTGCTGA AAAAGCACGA GGCATTTGAG
    ACAGACTTTA CAGTGCACAA AGACAGAGTA CATGATGTAT GTGCCAACGG GGAAGACCTG
    ATACAAAAGA ACAATCACCA TGTGGAGAAT ATTAGCGCCA AAATGAAGGG TCTCCGAGGG
    AAGGTGGCAG AACTAGAGAA GGCGGCTGCA CAGCGCAAGG CCAAACTTGA TGAGAATTCA
    GCTTTCCTGC AGTTCAACTG GAAGGCGGAT GTTGTGGAGT CCTGGATCGG TGAAAAAGAG
    AACAGCCTGA AGACTGATGA TTATGGCAGA GACCTGTCCT CTGTGCAGAC CCTTCTCACT
    AAGCAGGAAA CATTTGATGC TGGGCTGCAA GCTTTCCAGC AAGAGGGCAT TACCAACATC
    ACTGCACTGA AAGACCAGCT TCTGGCTGCA AAGCATGTGC AGTCCCGGGC CATAGAGTCT
    CGTCACGCTT CTCTGATGAA GCGGTGGAAC CAGTTGTTGG ATAACTCTGC TGCCCGCAAA
    AAGAAGTTAT TGGAGGCCCA GGAACACTAC AGAAAGGTTG AGGACTTGTT CCTAACATTT
    GCCAAGAAAG CCTCTGCCTT CAACAGCTGG TTTGAGAATG CCGAGGAGGA TTTGACTGAT
    CCTGTTCGCT GCAACTCCCT GGAGGAAATC AAGGCGCTTC GCGAGGCACA TGACGCCTTC
    CGCAATTCCC TCAGCTCCGC ACAGACAGAT TTCAACCAGC TGGCCGAACT GGACAGGCAA
    ATAAAGGGCT ACCGGGTAGC CTCCAATCCC TACACGTGGT TCACGATGGA GGCTTTGGAG
    GAGACATGGC GCAACCTGCA GAAAATCATT AAGGAACGAG AACTTGAGCT ACAAAAAGAA
    CAACGTAGGC AGGAAGAGAA CGATAAGCTC CGGCAAGAGT TTGCTCAGCA CGCCAATGCT
    TTCCACCAGT GGATTCAGGA GACAAGGACC TACCTTCTGG ACGGAATAGC CTATCGCAGA
    GTCATACGTG TGTATCAGTA TGAAGTTGGG GATGATCTTT CTGGAAGGTC CTGTATGGTG
    GAAGAATCTG GAACACTGGA GTCACAACTT GAAGCTACAA AGAGAAAGCA TCAGGAGATC
    CGGGCCATGA GGAGTCAACT GAAGAAGATA GAAGATCTTG GCGCAGCCAT GGAAGAGGCC
    CTGATCTTGG ATAATAAATA CACAGAACAC AGCACTGTTG GGCTGGCACA GCAATGGGAC
    CAACTGGATC AGCTAGGCAT GAGAATGCAA CACAACCTAG AGCAACAGAT CCAGGCCAGA
    AACACAACTG GGGTCACAGA GGAAGCACTC AAAGAATTCA GCATGATGTT CAAGCATTTC
    GACAAAGACA AGTCTGGCCG CCTCAACCAC CAAGAATTCA AATCTTGTCT GCGGTCCCTT
    GGCTATGATC TGCCCATGGT AGAAGAAGGA GAACCAGACC CAGAGTTTGA AGCCATTTTG
    GACACTGTAG ATCCCAACAG AGATGGGCAC GTTTCTCTAC AGGAGTACAT GGCTTTCATG
    ATTAGCAGAG AAACTGAAAA CGTCAAGTCC AGTGAGGAGA TAGAGAGCGC CTTCCGAGCA
    CTGAGTACAG ACCAGAAACC TTATGTCACA AAGGAAGAAC TATACCAGAA CCTGACACGC
    GAACAGGCAG ATTACTGCAT CTCGCATATG AAACCCTACA TGGACAGCAA GGGCCGCGAA
    CTGCCCTCTG CATACGACTA CGTGGAATTC ACGCGCTCGC TATTTGTGAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa63343
    Clone ID Related Accession (Same CDS sequence) XM_031906803.1
    Accession Version XM_031906803.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7479bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGGATTCAG GTGGCGTGAA AGTGCTGGAG ACAGCGGAGG ACATCCAGGA AAGGCGCCAG 
    CAAGTCCTAG ACCGATACCT GCGGTTTAAG GAACTCTCTG TACTGAGACG TCAGAAGTTG
    GAGGATTCTT ACCGCTTCCA GTTCTTCCAG CGAGATGCTG ATGAACTGGA GAAATGGATC
    CAGGAGAAAC TCCAGATCGC ATCTGATGAG AACTACAAAG ATCCAACCAA TCTGCAGGGC
    AAATTGCAGA AACACCAGGC TTTTGAGGCA GAAGTACAGG CCAATGCAGG GGCAATTGTC
    AAACTGGACG AAACTGGAAA CCTGATGATT TCAGAAGGAC ATTTTTCATC AGAGACCATC
    AGAACCCGCC TGGTAGAGTT GCATCGTCTG TGGGAGCTGC TGCTCTTAAA GATGCAAGAG
    AAAGGGGTTA AATTACAACA GGCGCAGAAG CTTGTTCAGT ATCTACGGGA ATGTGAAGAT
    GTCCTAGACT GGATTAATGA CAAGGAGGCT ATTGTAACAT CTGAGGAGCT TGGTCAGGAT
    TTGGAACATG TGGAGGTCTT ACAGAAGAAG TTTGAAGAGT TTCAGACTGA TCTGGCAGCT
    CATGAAGAAA GAGTGAATGA AGTCAACCAG TTTGCAAGTA AACTGGCCCA GGAGGAACAC
    CCGGAGCTGG AGCTAATAAA GACCAAGCAG GATGAAGTCA ATGCAAGTTG GCAGCGCCTC
    AAAGGGCTGG CACTTCAGAG ACAAGGGAAG TTATTTGGAG CTGCAGAAGT TCAGCGTTTT
    AACAGAGATG TTGATGAAAC CATTGGATGG ATAAGAGAGA AGGAGCAGCT GATGGCATCT
    GATGACTTTG GCCGAGACCT GGCTAGCGTT CAAGCTCTCC TACGTAAACA CGAGGGTCTG
    GAGAGAGACC TTGCAGCACT GGAGGAAAAG GTAAAGGCCT TGTGTGCAGA ATCAGACCGT
    CTGCAGCAAT CCCATCCCAT GAACGCCCCT CAAATTCAAG TGAAAGGAGA AGAACTGATA
    ACCAACTGGG AGCAAATACG CACATTGGCA GCAGAACGAC ATGCTCGACT CAATGACTCT
    TACAAGCTTC AGCGTTTCCT TGCAGATTTT AGAGATCTGA CTAGCTGGGT GACTGAAATG
    AAAGCTCTGA TTAATGCCGA TGAGCTTGCC AACGATGTGG CTGGCGCAGA GGCTCTGCTT
    GACCGACACC AGGAACATAA GGGGGAAATT GATGCTCATG AAGATAGCTT TAAAGCTGCA
    GATGCTGCAG GTCAGGCCCT TCTGAACGGT GGCCACTACG CCTCAGATGA AGTCCGAGAA
    AAGCTTACTA TCTTAGCAGA AGAAAGGGCA GCTCTTCTAG AGCTTTGGGA GCTCCGTCGT
    CAGCAGTATG AACAGTGCAT GGACCTGCAG CTTTTTTACA GAGACACCGA ACAGGTGGAC
    AACTGGATGA GCAAACAGGA AGCTTTCCTG CTTAACGAGG ACTTGGGTGA TTCTCTAGAC
    AGTGTGGAAG CTCTTCTGAA AAAACATGAG GACTTTGAGA AGTCTCTTAG CGCCCAGGAA
    GAGAAAATCA CAGCTCTGGA TGAGTTTGCT ACAAAACTAA TTCAAAATAA CCACTATGCC
    AAGGAAGATG TGGCTACCCG CAGAGATGCT CTGCTGAGCC GCCGCAATGC ACTTCATGAA
    CGTGCACTCT ACCGCCGTAC TCAACTGGCA GACTCCTTTC ACCTGCAGCA GTTCTTTAGG
    GACTCTGACG AGCTGAAAAG TTGGATTAAT GAAAAGATGA AGACTGCAAC GGATGAGGCA
    TACAAGGATC CCTCCAACCT CCAGGGTAAG GTGCAGAAAC ACCAAGCTTT TGAGGCTGAA
    CTCTCCGCTA ACCAAAGCCG TATAGATGCC CTGGAGAATT CTGGGCAGAA ATTGATTGAT
    GTCAACCATT ATGCATCAGA TGAGGTGGCA GCACGTATGA GTGAGGTCAT CACTTTGTGG
    AAGAAGCTGC TGGAGGCCAC TGAATTGAAG GGGATCAAGC TGCGCGAAGC CAACCAGCAG
    CAGCAGTTCA ACCGTAATGT GGAGGATATA GAGCTGTGGC TGTATGAGGT TGAGGGGCAT
    TTGGCTTCAG ATGACTATGG AAAAGACCTG ACCAATGTTC AAAACTTGCA GAAAAAACAC
    GCCTTGCTGG AGGCTGATGT TGCAGCTCAC CAGGACCGTA TCGATGGCAT CACTATCCAA
    GCCCGTCAGT TCCAAGAAGC TGGTCATTTT GATGCTGACA ATATCAGGAA GAAACAGGAG
    GCCCTTGTTA ATAGATATGA AGCTTTGAAA GAACCCATGG TTGCACGCAA GCAGAAGCTG
    TCAGACTCTC TGCGTCTTCA ACAGCTTTTC AGGGATGTTG AAGATGAGGA AACCTGGATA
    AGGGAGAAGG AACCCATTGC AGCGTCCACC AACAGAGGCA AGGATTTGAT TGGAGTCCAG
    AATCTCCTGA AGAAACACCA GGCTTTGCAG GCTGAAATTG CAGGGCACGA ACCACGAATT
    AAAGCTGTTA CCCAGAAAGG AGAATCTATG ATCTCTGAAG GCCACTTTGC TTCTGATGAA
    GTGAAGGGCA AATTAAGGGA GCTGAATGAT AAGTGGGCCA CCCTGAAGAA CAAAGCATCT
    CAGCGCCGAC AGGACCTTGA AGATTCTCTG CAAGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCAGAGT CCTGGATGAG AGAGAAGGAA CCTATTGTTG GAAGCACCGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC CCTTCTGAAG AAACATGAAG CGCTTATGTC AGATCTGCGA
    GCATACGGAA GCAGTATTCA GGGACTTAGA GAGCAGGCCC AGGCCTGCAG GCAACAAGTT
    GCTCCAACTG ATGATGAAAC TGGAAAGGAG CTGGTGCTGG CATTGTATGA TTATCAAGAG
    AAGAGCCCTA GGGAGGTGAC AATGAAGAAA GGAGACATCT TGACCTTGCT AAACAGCACA
    AATAAGGATT GGTGGAAGGT GGAAGTGAAT GATCGTCAGG GCTTTGTACC TGCTGCGTAT
    GTGAAAAAGC TGGACCCAGC ACAGTCCGCA TCCCGGGAAA ACTTGCTGGA GGAACAGGGA
    AGCATCGCTC TCCGTCAGGA GCAGATCGAT GGCCAGGCTC ACATGTCTAA GGAAGCTGGC
    AGTGTTTCTC TGCGTATGAA TCAGGTTGAA GAATTGTATC ATAATATTTT GGAGATGGGA
    GAGAAGCGCA AAGATATGTT AGAAAAGAGC TGCAAGAAGT TTATGCTGTT CCGTGAGGCA
    AATGAGCTGC AGCAGTGGAT TAACGAGAAA GAAGCATCAC TCACTAACGA AGAGGTCGGG
    GCTGACCTGG AACAAGTGGA AGTGCTGCAG AAGAAGTTTG ATGATTTCCA GAAGGATCTG
    AAAGCAAATG AGTCACGCTT GAAGGACATC AACAAGGTGG CCGACGAGCT TGAATCTGAA
    GGCCTCATTA CAGAGGAAGT CCAGCCGGTG CAGCATCAGG AGGTGTATGG GATGCAGCGG
    GATGAAGCAG ACTCCAAATC GGCATCTCCA TGGAAGTCTG TTCGCACAGC TGTGCTCGCG
    GTGGCCACCT TCAACTCTAT CAAGGACTTG AACGAGCGCT GGAAATCACT TCAACAGCTG
    GCAGAAGAAA GGAGTCAGCT TCTTGGCAGT GCCCATGAAG TACAGAGATT CCACAGAGAT
    GCTGATGAGA CAAAAGAATG GATCGAAGAG AAGAACCAGG CTTTGAATAC AGACAGCTAT
    GGACATGACT TGGCCAGTGT GCAGGCTCTG CAACGAAAAC ATGAAGGCTT TGAGAGAGAC
    CTCGCCGCTC TGGGAGATAA GGTGAACTCT TTGGGAGAAA CTGCTGAGCG CCTGATCCAG
    TCACACCCTG AGTCTGCAGA TGACATTCAG GAAAAATGCA CAGAGCTGAA TCAGGCCTGG
    AACAGCCTTG GCACCCGGGC AAACCAGCGC AAGGAGAAAC TAGGCGATTC TCATGATCTG
    CAACGCTTCC TAAGTGACTT CAGAGATCTC ATGTCCTGGA TCAATGGAAT TCGGGGCTTG
    GTGTCATCAG ATGAACTTGC TAGAGATGTG ACTGGTGCAG AGGCTTTGCT GGAACGACAC
    CAGGAGCACA GGACTGAGAT TGATGCCAGA GCAGGCACTT TTCAGGCCTT TGAGCAGTTT
    GGGCAGCAAC TTCTGGCTCG TGGTCATTAT GATAGTCCTG AAATCAAGGA GAAGCTGGAT
    ATCTTGGAAC AGGAACGGGC AAGTTTGGAA AAGGCTTGGG TGGAACGCAG AATGATGTTG
    GATCAGTGCT TGGAGCTGCA GCTCTTCAAC AGAGACTGCG AGCAGGCAGA GAACTGGATG
    GCAGCCCGTG AAGCCTTCCT GAACAGTGAG GATAAAGGGG ATTCCCTGGA CAGTGTGGAG
    GCCCTTATCA AGAAACATGA AGACTTTGAT AAAGCCATTA ATGTCCAGGA AGAGAAGATT
    GCTGCCCTCC AGGCCTTCGC CGAACAATTG ATAATTGCTG ACCACTATGC CAAAGGAGAT
    ATTGCCTCCC GTCGCAATGA AGTCCTGGAC AGGTGGCGCC GTCTCAAGGC TCAGATGATT
    GAGAAGAGAT CCAAACTTGG GGAATCTCAG ACTCTGCAGC AGTTCAGCAG GGACGTCGAT
    GAGATCGAAG CCTGGATCAG CGAGAAATTG CAGACCGCCA GTGACGAGTC CTACAAAGAC
    CCCACAAACA TCCAGAGCAA ACACCAGAAG CACCAGGCTT TCGAAGCTGA GCTCCATGCC
    AACGCTGACC GTATTCGTGG AGTAGTAGAT ATGGGGAACT CCCTTATCGA ACGAGGTGCC
    TGTGCTGGCA GCGAGGATGC GGTGAAGGCT CGCCTAAGCG CACTCGCTGA TCAATGGCAG
    TTCCTTGTCA GCAAATCCGC AGAGAAGAGC CAGAAACTGA AGGAGGCAAA CAAGCAGCAG
    AACTTTAACA CTGGCATCAA AGACTTTGAT TTCTGGTTAA CAGAGGTGGA GGCTTTGCTG
    GCATCTGAAG ACTATGGGAA GGACTTGGCA TCTGTTAACA ACCTGCTGAA GAAACACCAG
    CTTCTGGAAG CTGACATCTC TGCTCATGAG GACCGCCTTA AAGATCTTAA TGGGCAGGCT
    GATAGCCTGA TGACCAGCAG CGCCTTTGAC ACCACCCAAG TAAAGGAGAA AAGGGACGCC
    ATCAACGACC GCTTCCAGCG TATCAAAAGC ATGGCCACCG CACGCCGCAG CAGGCTGAAT
    GAGTCCCACC GTCTGCACCA GTTCTTCAGA GATCTTGATG ATGAGGAATC ATGGATCAAG
    GAAAAGAAAC TTTTGGTCGG ATCTGATGAC TATGGACGTG ACTTGACTGG AGTGCAAAAT
    CTGAGGAAGA AACACAAGAG ATTAGAAGCT GAACTGGCAG CTCATGAGCC AGCCATTCAG
    GGTGTGCTGG ACACTGGGAA GAAGCTTGCA GATGACAACA CTATTGGGAA AGATGAGATT
    CAGCAGCGAC TGGCACAGTT TCTAGAGCAC TGGAAGGAGC TGAAGGAACA AGCAGCGGCT
    CGTGGTCAGA GACTGGAGGA ATCTCTTGAG TACCAACAAT TTGTAGCCAA TGTGGAGGAA
    GAGGAAGCCT GGATTAATGA GAAGATGACT CTAGTGGCCA GTGAAGACTA TGGTGACACA
    CTGGCTGCCA TTCAGGGATT GCTGAAAAAG CACGAGGCAT TTGAGACAGA CTTTACAGTG
    CACAAAGACA GAGTACATGA TGTATGTGCC AACGGGGAAG ACCTGATACA AAAGAACAAT
    CACCATGTGG AGAATATTAG CGCCAAAATG AAGGGTCTCC GAGGGAAGGT GGCAGAACTA
    GAGAAGGCGG CTGCACAGCG CAAGGCCAAA CTTGATGAGA ATTCAGCTTT CCTGCAGTTC
    AACTGGAAGG CGGATGTTGT GGAGTCCTGG ATCGGTGAAA AAGAGAACAG CCTGAAGACT
    GATGATTATG GCAGAGACCT GTCCTCTGTG CAGACCCTTC TCACTAAGCA GGAAACATTT
    GATGCTGGGC TGCAAGCTTT CCAGCAAGAG GGCATTACCA ACATCACTGC ACTGAAAGAC
    CAGCTTCTGG CTGCAAAGCA TGTGCAGTCC CGGGCCATAG AGTCTCGTCA CGCTTCTCTG
    ATGAAGCGGT GGAACCAGTT GTTGGATAAC TCTGCTGCCC GCAAAAAGAA GTTATTGGAG
    GCCCAGGAAC ACTACAGAAA GGTTGAGGAC TTGTTCCTAA CATTTGCCAA GAAAGCCTCT
    GCCTTCAACA GCTGGTTTGA GAATGCCGAG GAGGATTTGA CTGATCCTGT TCGCTGCAAC
    TCCCTGGAGG AAATCAAGGC GCTTCGCGAG GCACATGACG CCTTCCGCAA TTCCCTCAGC
    TCCGCACAGA CAGATTTCAA CCAGCTGGCC GAACTGGACA GGCAAATAAA GGGCTACCGG
    GTAGCCTCCA ATCCCTACAC GTGGTTCACG ATGGAGGCTT TGGAGGAGAC ATGGCGCAAC
    CTGCAGAAAA TCATTAAGGA ACGAGAACTT GAGCTACAAA AAGAACAACG TAGGCAGGAA
    GAGAACGATA AGCTCCGGCA AGAGTTTGCT CAGCACGCCA ATGCTTTCCA CCAGTGGATT
    CAGGAGACAA GGACCTACCT TCTGGACGGA ATAGCCTATC GCAGAGTCAT ACGTGTGTAT
    CAGTATGAAG TTGGGGATGA TCTTTCTGGA AGGTCCTGTA TGGTGGAAGA ATCTGGAACA
    CTGGAGTCAC AACTTGAAGC TACAAAGAGA AAGCATCAGG AGATCCGGGC CATGAGGAGT
    CAACTGAAGA AGATAGAAGA TCTTGGCGCA GCCATGGAAG AGGCCCTGAT CTTGGATAAT
    AAATACACAG AACACAGCAC TGTTGGGCTG GCACAGCAAT GGGACCAACT GGATCAGCTA
    GGCATGAGAA TGCAACACAA CCTAGAGCAA CAGATCCAGG CCAGAAACAC AACTGGGGTC
    ACAGAGGAAG CACTCAAAGA ATTCAGCATG ATGTTCAAGC ATTTCGACAA AGACAAGTCT
    GGCCGCCTCA ACCACCAAGA ATTCAAATCT TGTCTGCGGT CCCTTGGCTA TGATCTGCCC
    ATGGTAGAAG AAGGAGAACC AGACCCAGAG TTTGAAGCCA TTTTGGACAC TGTAGATCCC
    AACAGAGATG GGCACGTTTC TCTACAGGAG TACATGGCTT TCATGATTAG CAGAGAAACT
    GAAAACGTCA AGTCCAGTGA GGAGATAGAG AGCGCCTTCC GAGCACTGAG TACAGACCAG
    AAACCTTATG TCACAAAGGA AGAACTATAC CAGAACCTGA CACGCGAACA GGCAGATTAC
    TGCATCTCGC ATATGAAACC CTACATGGAC AGCAAGGGCC GCGAACTGCC CTCTGCATAC
    GACTACGTGG AATTCACGCG CTCGCTATTT GTGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031762663.1
    CDS4..7482
    Translation

    Target ORF information:

    RefSeq Version XM_031906803.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis spectrin, alpha, non-erythrocytic 1 (sptan1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031906803.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGGATTCAG GTGGCGTGAA AGTGCTGGAG ACAGCGGAGG ACATCCAGGA AAGGCGCCAG 
    CAAGTCCTAG ACCGATACCT GCGGTTTAAG GAACTCTCTG TACTGAGACG TCAGAAGTTG
    GAGGATTCTT ACCGCTTCCA GTTCTTCCAG CGAGATGCTG ATGAACTGGA GAAATGGATC
    CAGGAGAAAC TCCAGATCGC ATCTGATGAG AACTACAAAG ATCCAACCAA TCTGCAGGGC
    AAATTGCAGA AACACCAGGC TTTTGAGGCA GAAGTACAGG CCAATGCAGG GGCAATTGTC
    AAACTGGACG AAACTGGAAA CCTGATGATT TCAGAAGGAC ATTTTTCATC AGAGACCATC
    AGAACCCGCC TGGTAGAGTT GCATCGTCTG TGGGAGCTGC TGCTCTTAAA GATGCAAGAG
    AAAGGGGTTA AATTACAACA GGCGCAGAAG CTTGTTCAGT ATCTACGGGA ATGTGAAGAT
    GTCCTAGACT GGATTAATGA CAAGGAGGCT ATTGTAACAT CTGAGGAGCT TGGTCAGGAT
    TTGGAACATG TGGAGGTCTT ACAGAAGAAG TTTGAAGAGT TTCAGACTGA TCTGGCAGCT
    CATGAAGAAA GAGTGAATGA AGTCAACCAG TTTGCAAGTA AACTGGCCCA GGAGGAACAC
    CCGGAGCTGG AGCTAATAAA GACCAAGCAG GATGAAGTCA ATGCAAGTTG GCAGCGCCTC
    AAAGGGCTGG CACTTCAGAG ACAAGGGAAG TTATTTGGAG CTGCAGAAGT TCAGCGTTTT
    AACAGAGATG TTGATGAAAC CATTGGATGG ATAAGAGAGA AGGAGCAGCT GATGGCATCT
    GATGACTTTG GCCGAGACCT GGCTAGCGTT CAAGCTCTCC TACGTAAACA CGAGGGTCTG
    GAGAGAGACC TTGCAGCACT GGAGGAAAAG GTAAAGGCCT TGTGTGCAGA ATCAGACCGT
    CTGCAGCAAT CCCATCCCAT GAACGCCCCT CAAATTCAAG TGAAAGGAGA AGAACTGATA
    ACCAACTGGG AGCAAATACG CACATTGGCA GCAGAACGAC ATGCTCGACT CAATGACTCT
    TACAAGCTTC AGCGTTTCCT TGCAGATTTT AGAGATCTGA CTAGCTGGGT GACTGAAATG
    AAAGCTCTGA TTAATGCCGA TGAGCTTGCC AACGATGTGG CTGGCGCAGA GGCTCTGCTT
    GACCGACACC AGGAACATAA GGGGGAAATT GATGCTCATG AAGATAGCTT TAAAGCTGCA
    GATGCTGCAG GTCAGGCCCT TCTGAACGGT GGCCACTACG CCTCAGATGA AGTCCGAGAA
    AAGCTTACTA TCTTAGCAGA AGAAAGGGCA GCTCTTCTAG AGCTTTGGGA GCTCCGTCGT
    CAGCAGTATG AACAGTGCAT GGACCTGCAG CTTTTTTACA GAGACACCGA ACAGGTGGAC
    AACTGGATGA GCAAACAGGA AGCTTTCCTG CTTAACGAGG ACTTGGGTGA TTCTCTAGAC
    AGTGTGGAAG CTCTTCTGAA AAAACATGAG GACTTTGAGA AGTCTCTTAG CGCCCAGGAA
    GAGAAAATCA CAGCTCTGGA TGAGTTTGCT ACAAAACTAA TTCAAAATAA CCACTATGCC
    AAGGAAGATG TGGCTACCCG CAGAGATGCT CTGCTGAGCC GCCGCAATGC ACTTCATGAA
    CGTGCACTCT ACCGCCGTAC TCAACTGGCA GACTCCTTTC ACCTGCAGCA GTTCTTTAGG
    GACTCTGACG AGCTGAAAAG TTGGATTAAT GAAAAGATGA AGACTGCAAC GGATGAGGCA
    TACAAGGATC CCTCCAACCT CCAGGGTAAG GTGCAGAAAC ACCAAGCTTT TGAGGCTGAA
    CTCTCCGCTA ACCAAAGCCG TATAGATGCC CTGGAGAATT CTGGGCAGAA ATTGATTGAT
    GTCAACCATT ATGCATCAGA TGAGGTGGCA GCACGTATGA GTGAGGTCAT CACTTTGTGG
    AAGAAGCTGC TGGAGGCCAC TGAATTGAAG GGGATCAAGC TGCGCGAAGC CAACCAGCAG
    CAGCAGTTCA ACCGTAATGT GGAGGATATA GAGCTGTGGC TGTATGAGGT TGAGGGGCAT
    TTGGCTTCAG ATGACTATGG AAAAGACCTG ACCAATGTTC AAAACTTGCA GAAAAAACAC
    GCCTTGCTGG AGGCTGATGT TGCAGCTCAC CAGGACCGTA TCGATGGCAT CACTATCCAA
    GCCCGTCAGT TCCAAGAAGC TGGTCATTTT GATGCTGACA ATATCAGGAA GAAACAGGAG
    GCCCTTGTTA ATAGATATGA AGCTTTGAAA GAACCCATGG TTGCACGCAA GCAGAAGCTG
    TCAGACTCTC TGCGTCTTCA ACAGCTTTTC AGGGATGTTG AAGATGAGGA AACCTGGATA
    AGGGAGAAGG AACCCATTGC AGCGTCCACC AACAGAGGCA AGGATTTGAT TGGAGTCCAG
    AATCTCCTGA AGAAACACCA GGCTTTGCAG GCTGAAATTG CAGGGCACGA ACCACGAATT
    AAAGCTGTTA CCCAGAAAGG AGAATCTATG ATCTCTGAAG GCCACTTTGC TTCTGATGAA
    GTGAAGGGCA AATTAAGGGA GCTGAATGAT AAGTGGGCCA CCCTGAAGAA CAAAGCATCT
    CAGCGCCGAC AGGACCTTGA AGATTCTCTG CAAGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCAGAGT CCTGGATGAG AGAGAAGGAA CCTATTGTTG GAAGCACCGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC CCTTCTGAAG AAACATGAAG CGCTTATGTC AGATCTGCGA
    GCATACGGAA GCAGTATTCA GGGACTTAGA GAGCAGGCCC AGGCCTGCAG GCAACAAGTT
    GCTCCAACTG ATGATGAAAC TGGAAAGGAG CTGGTGCTGG CATTGTATGA TTATCAAGAG
    AAGAGCCCTA GGGAGGTGAC AATGAAGAAA GGAGACATCT TGACCTTGCT AAACAGCACA
    AATAAGGATT GGTGGAAGGT GGAAGTGAAT GATCGTCAGG GCTTTGTACC TGCTGCGTAT
    GTGAAAAAGC TGGACCCAGC ACAGTCCGCA TCCCGGGAAA ACTTGCTGGA GGAACAGGGA
    AGCATCGCTC TCCGTCAGGA GCAGATCGAT GGCCAGGCTC ACATGTCTAA GGAAGCTGGC
    AGTGTTTCTC TGCGTATGAA TCAGGTTGAA GAATTGTATC ATAATATTTT GGAGATGGGA
    GAGAAGCGCA AAGATATGTT AGAAAAGAGC TGCAAGAAGT TTATGCTGTT CCGTGAGGCA
    AATGAGCTGC AGCAGTGGAT TAACGAGAAA GAAGCATCAC TCACTAACGA AGAGGTCGGG
    GCTGACCTGG AACAAGTGGA AGTGCTGCAG AAGAAGTTTG ATGATTTCCA GAAGGATCTG
    AAAGCAAATG AGTCACGCTT GAAGGACATC AACAAGGTGG CCGACGAGCT TGAATCTGAA
    GGCCTCATTA CAGAGGAAGT CCAGCCGGTG CAGCATCAGG AGGTGTATGG GATGCAGCGG
    GATGAAGCAG ACTCCAAATC GGCATCTCCA TGGAAGTCTG TTCGCACAGC TGTGCTCGCG
    GTGGCCACCT TCAACTCTAT CAAGGACTTG AACGAGCGCT GGAAATCACT TCAACAGCTG
    GCAGAAGAAA GGAGTCAGCT TCTTGGCAGT GCCCATGAAG TACAGAGATT CCACAGAGAT
    GCTGATGAGA CAAAAGAATG GATCGAAGAG AAGAACCAGG CTTTGAATAC AGACAGCTAT
    GGACATGACT TGGCCAGTGT GCAGGCTCTG CAACGAAAAC ATGAAGGCTT TGAGAGAGAC
    CTCGCCGCTC TGGGAGATAA GGTGAACTCT TTGGGAGAAA CTGCTGAGCG CCTGATCCAG
    TCACACCCTG AGTCTGCAGA TGACATTCAG GAAAAATGCA CAGAGCTGAA TCAGGCCTGG
    AACAGCCTTG GCACCCGGGC AAACCAGCGC AAGGAGAAAC TAGGCGATTC TCATGATCTG
    CAACGCTTCC TAAGTGACTT CAGAGATCTC ATGTCCTGGA TCAATGGAAT TCGGGGCTTG
    GTGTCATCAG ATGAACTTGC TAGAGATGTG ACTGGTGCAG AGGCTTTGCT GGAACGACAC
    CAGGAGCACA GGACTGAGAT TGATGCCAGA GCAGGCACTT TTCAGGCCTT TGAGCAGTTT
    GGGCAGCAAC TTCTGGCTCG TGGTCATTAT GATAGTCCTG AAATCAAGGA GAAGCTGGAT
    ATCTTGGAAC AGGAACGGGC AAGTTTGGAA AAGGCTTGGG TGGAACGCAG AATGATGTTG
    GATCAGTGCT TGGAGCTGCA GCTCTTCAAC AGAGACTGCG AGCAGGCAGA GAACTGGATG
    GCAGCCCGTG AAGCCTTCCT GAACAGTGAG GATAAAGGGG ATTCCCTGGA CAGTGTGGAG
    GCCCTTATCA AGAAACATGA AGACTTTGAT AAAGCCATTA ATGTCCAGGA AGAGAAGATT
    GCTGCCCTCC AGGCCTTCGC CGAACAATTG ATAATTGCTG ACCACTATGC CAAAGGAGAT
    ATTGCCTCCC GTCGCAATGA AGTCCTGGAC AGGTGGCGCC GTCTCAAGGC TCAGATGATT
    GAGAAGAGAT CCAAACTTGG GGAATCTCAG ACTCTGCAGC AGTTCAGCAG GGACGTCGAT
    GAGATCGAAG CCTGGATCAG CGAGAAATTG CAGACCGCCA GTGACGAGTC CTACAAAGAC
    CCCACAAACA TCCAGAGCAA ACACCAGAAG CACCAGGCTT TCGAAGCTGA GCTCCATGCC
    AACGCTGACC GTATTCGTGG AGTAGTAGAT ATGGGGAACT CCCTTATCGA ACGAGGTGCC
    TGTGCTGGCA GCGAGGATGC GGTGAAGGCT CGCCTAAGCG CACTCGCTGA TCAATGGCAG
    TTCCTTGTCA GCAAATCCGC AGAGAAGAGC CAGAAACTGA AGGAGGCAAA CAAGCAGCAG
    AACTTTAACA CTGGCATCAA AGACTTTGAT TTCTGGTTAA CAGAGGTGGA GGCTTTGCTG
    GCATCTGAAG ACTATGGGAA GGACTTGGCA TCTGTTAACA ACCTGCTGAA GAAACACCAG
    CTTCTGGAAG CTGACATCTC TGCTCATGAG GACCGCCTTA AAGATCTTAA TGGGCAGGCT
    GATAGCCTGA TGACCAGCAG CGCCTTTGAC ACCACCCAAG TAAAGGAGAA AAGGGACGCC
    ATCAACGACC GCTTCCAGCG TATCAAAAGC ATGGCCACCG CACGCCGCAG CAGGCTGAAT
    GAGTCCCACC GTCTGCACCA GTTCTTCAGA GATCTTGATG ATGAGGAATC ATGGATCAAG
    GAAAAGAAAC TTTTGGTCGG ATCTGATGAC TATGGACGTG ACTTGACTGG AGTGCAAAAT
    CTGAGGAAGA AACACAAGAG ATTAGAAGCT GAACTGGCAG CTCATGAGCC AGCCATTCAG
    GGTGTGCTGG ACACTGGGAA GAAGCTTGCA GATGACAACA CTATTGGGAA AGATGAGATT
    CAGCAGCGAC TGGCACAGTT TCTAGAGCAC TGGAAGGAGC TGAAGGAACA AGCAGCGGCT
    CGTGGTCAGA GACTGGAGGA ATCTCTTGAG TACCAACAAT TTGTAGCCAA TGTGGAGGAA
    GAGGAAGCCT GGATTAATGA GAAGATGACT CTAGTGGCCA GTGAAGACTA TGGTGACACA
    CTGGCTGCCA TTCAGGGATT GCTGAAAAAG CACGAGGCAT TTGAGACAGA CTTTACAGTG
    CACAAAGACA GAGTACATGA TGTATGTGCC AACGGGGAAG ACCTGATACA AAAGAACAAT
    CACCATGTGG AGAATATTAG CGCCAAAATG AAGGGTCTCC GAGGGAAGGT GGCAGAACTA
    GAGAAGGCGG CTGCACAGCG CAAGGCCAAA CTTGATGAGA ATTCAGCTTT CCTGCAGTTC
    AACTGGAAGG CGGATGTTGT GGAGTCCTGG ATCGGTGAAA AAGAGAACAG CCTGAAGACT
    GATGATTATG GCAGAGACCT GTCCTCTGTG CAGACCCTTC TCACTAAGCA GGAAACATTT
    GATGCTGGGC TGCAAGCTTT CCAGCAAGAG GGCATTACCA ACATCACTGC ACTGAAAGAC
    CAGCTTCTGG CTGCAAAGCA TGTGCAGTCC CGGGCCATAG AGTCTCGTCA CGCTTCTCTG
    ATGAAGCGGT GGAACCAGTT GTTGGATAAC TCTGCTGCCC GCAAAAAGAA GTTATTGGAG
    GCCCAGGAAC ACTACAGAAA GGTTGAGGAC TTGTTCCTAA CATTTGCCAA GAAAGCCTCT
    GCCTTCAACA GCTGGTTTGA GAATGCCGAG GAGGATTTGA CTGATCCTGT TCGCTGCAAC
    TCCCTGGAGG AAATCAAGGC GCTTCGCGAG GCACATGACG CCTTCCGCAA TTCCCTCAGC
    TCCGCACAGA CAGATTTCAA CCAGCTGGCC GAACTGGACA GGCAAATAAA GGGCTACCGG
    GTAGCCTCCA ATCCCTACAC GTGGTTCACG ATGGAGGCTT TGGAGGAGAC ATGGCGCAAC
    CTGCAGAAAA TCATTAAGGA ACGAGAACTT GAGCTACAAA AAGAACAACG TAGGCAGGAA
    GAGAACGATA AGCTCCGGCA AGAGTTTGCT CAGCACGCCA ATGCTTTCCA CCAGTGGATT
    CAGGAGACAA GGACCTACCT TCTGGACGGA ATAGCCTATC GCAGAGTCAT ACGTGTGTAT
    CAGTATGAAG TTGGGGATGA TCTTTCTGGA AGGTCCTGTA TGGTGGAAGA ATCTGGAACA
    CTGGAGTCAC AACTTGAAGC TACAAAGAGA AAGCATCAGG AGATCCGGGC CATGAGGAGT
    CAACTGAAGA AGATAGAAGA TCTTGGCGCA GCCATGGAAG AGGCCCTGAT CTTGGATAAT
    AAATACACAG AACACAGCAC TGTTGGGCTG GCACAGCAAT GGGACCAACT GGATCAGCTA
    GGCATGAGAA TGCAACACAA CCTAGAGCAA CAGATCCAGG CCAGAAACAC AACTGGGGTC
    ACAGAGGAAG CACTCAAAGA ATTCAGCATG ATGTTCAAGC ATTTCGACAA AGACAAGTCT
    GGCCGCCTCA ACCACCAAGA ATTCAAATCT TGTCTGCGGT CCCTTGGCTA TGATCTGCCC
    ATGGTAGAAG AAGGAGAACC AGACCCAGAG TTTGAAGCCA TTTTGGACAC TGTAGATCCC
    AACAGAGATG GGCACGTTTC TCTACAGGAG TACATGGCTT TCATGATTAG CAGAGAAACT
    GAAAACGTCA AGTCCAGTGA GGAGATAGAG AGCGCCTTCC GAGCACTGAG TACAGACCAG
    AAACCTTATG TCACAAAGGA AGAACTATAC CAGAACCTGA CACGCGAACA GGCAGATTAC
    TGCATCTCGC ATATGAAACC CTACATGGAC AGCAAGGGCC GCGAACTGCC CTCTGCATAC
    GACTACGTGG AATTCACGCG CTCGCTATTT GTGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa63344
    Clone ID Related Accession (Same CDS sequence) XM_031906804.1
    Accession Version XM_031906804.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7434bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGATTCAG GTGGCGTGAA AGTGCTGGAG ACAGCGGAGG ACATCCAGGA AAGGCGCCAG 
    CAAGTCCTAG ACCGATACCT GCGGTTTAAG GAACTCTCTG TACTGAGACG TCAGAAGTTG
    GAGGATTCTT ACCGCTTCCA GTTCTTCCAG CGAGATGCTG ATGAACTGGA GAAATGGATC
    CAGGAGAAAC TCCAGATCGC ATCTGATGAG AACTACAAAG ATCCAACCAA TCTGCAGGGC
    AAATTGCAGA AACACCAGGC TTTTGAGGCA GAAGTACAGG CCAATGCAGG GGCAATTGTC
    AAACTGGACG AAACTGGAAA CCTGATGATT TCAGAAGGAC ATTTTTCATC AGAGACCATC
    AGAACCCGCC TGGTAGAGTT GCATCGTCTG TGGGAGCTGC TGCTCTTAAA GATGCAAGAG
    AAAGGGGTTA AATTACAACA GGCGCAGAAG CTTGTTCAGT ATCTACGGGA ATGTGAAGAT
    GTCCTAGACT GGATTAATGA CAAGGAGGCT ATTGTAACAT CTGAGGAGCT TGGTCAGGAT
    TTGGAACATG TGGAGGTCTT ACAGAAGAAG TTTGAAGAGT TTCAGACTGA TCTGGCAGCT
    CATGAAGAAA GAGTGAATGA AGTCAACCAG TTTGCAAGTA AACTGGCCCA GGAGGAACAC
    CCGGAGCTGG AGCTAATAAA GACCAAGCAG GATGAAGTCA ATGCAAGTTG GCAGCGCCTC
    AAAGGGCTGG CACTTCAGAG ACAAGGGAAG TTATTTGGAG CTGCAGAAGT TCAGCGTTTT
    AACAGAGATG TTGATGAAAC CATTGGATGG ATAAGAGAGA AGGAGCAGCT GATGGCATCT
    GATGACTTTG GCCGAGACCT GGCTAGCGTT CAAGCTCTCC TACGTAAACA CGAGGGTCTG
    GAGAGAGACC TTGCAGCACT GGAGGAAAAG GTAAAGGCCT TGTGTGCAGA ATCAGACCGT
    CTGCAGCAAT CCCATCCCAT GAACGCCCCT CAAATTCAAG TGAAAGGAGA AGAACTGATA
    ACCAACTGGG AGCAAATACG CACATTGGCA GCAGAACGAC ATGCTCGACT CAATGACTCT
    TACAAGCTTC AGCGTTTCCT TGCAGATTTT AGAGATCTGA CTAGCTGGGT GACTGAAATG
    AAAGCTCTGA TTAATGCCGA TGAGCTTGCC AACGATGTGG CTGGCGCAGA GGCTCTGCTT
    GACCGACACC AGGAACATAA GGGGGAAATT GATGCTCATG AAGATAGCTT TAAAGCTGCA
    GATGCTGCAG GTCAGGCCCT TCTGAACGGT GGCCACTACG CCTCAGATGA AGTCCGAGAA
    AAGCTTACTA TCTTAGCAGA AGAAAGGGCA GCTCTTCTAG AGCTTTGGGA GCTCCGTCGT
    CAGCAGTATG AACAGTGCAT GGACCTGCAG CTTTTTTACA GAGACACCGA ACAGGTGGAC
    AACTGGATGA GCAAACAGGA AGCTTTCCTG CTTAACGAGG ACTTGGGTGA TTCTCTAGAC
    AGTGTGGAAG CTCTTCTGAA AAAACATGAG GACTTTGAGA AGTCTCTTAG CGCCCAGGAA
    GAGAAAATCA CAGCTCTGGA TGAGTTTGCT ACAAAACTAA TTCAAAATAA CCACTATGCC
    AAGGAAGATG TGGCTACCCG CAGAGATGCT CTGCTGAGCC GCCGCAATGC ACTTCATGAA
    CGTGCACTCT ACCGCCGTAC TCAACTGGCA GACTCCTTTC ACCTGCAGCA GTTCTTTAGG
    GACTCTGACG AGCTGAAAAG TTGGATTAAT GAAAAGATGA AGACTGCAAC GGATGAGGCA
    TACAAGGATC CCTCCAACCT CCAGGGTAAG GTGCAGAAAC ACCAAGCTTT TGAGGCTGAA
    CTCTCCGCTA ACCAAAGCCG TATAGATGCC CTGGAGAATT CTGGGCAGAA ATTGATTGAT
    GTCAACCATT ATGCATCAGA TGAGGTGGCA GCACGTATGA GTGAGGTCAT CACTTTGTGG
    AAGAAGCTGC TGGAGGCCAC TGAATTGAAG GGGATCAAGC TGCGCGAAGC CAACCAGCAG
    CAGCAGTTCA ACCGTAATGT GGAGGATATA GAGCTGTGGC TGTATGAGGT TGAGGGGCAT
    TTGGCTTCAG ATGACTATGG AAAAGACCTG ACCAATGTTC AAAACTTGCA GAAAAAACAC
    GCCTTGCTGG AGGCTGATGT TGCAGCTCAC CAGGACCGTA TCGATGGCAT CACTATCCAA
    GCCCGTCAGT TCCAAGAAGC TGGTCATTTT GATGCTGACA ATATCAGGAA GAAACAGGAG
    GCCCTTGTTA ATAGATATGA AGCTTTGAAA GAACCCATGG TTGCACGCAA GCAGAAGCTG
    TCAGACTCTC TGCGTCTTCA ACAGCTTTTC AGGGATGTTG AAGATGAGGA AACCTGGATA
    AGGGAGAAGG AACCCATTGC AGCGTCCACC AACAGAGGCA AGGATTTGAT TGGAGTCCAG
    AATCTCCTGA AGAAACACCA GGCTTTGCAG GCTGAAATTG CAGGGCACGA ACCACGAATT
    AAAGCTGTTA CCCAGAAAGG AGAATCTATG ATCTCTGAAG GCCACTTTGC TTCTGATGAA
    GTGAAGGGCA AATTAAGGGA GCTGAATGAT AAGTGGGCCA CCCTGAAGAA CAAAGCATCT
    CAGCGCCGAC AGGACCTTGA AGATTCTCTG CAAGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCAGAGT CCTGGATGAG AGAGAAGGAA CCTATTGTTG GAAGCACCGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC CCTTCTGAAG AAACATGAAG CGCTTATGTC AGATCTGCGA
    GCATACGGAA GCAGTATTCA GGGACTTAGA GAGCAGGCCC AGGCCTGCAG GCAACAAGTT
    GCTCCAACTG ATGATGAAAC TGGAAAGGAG CTGGTGCTGG CATTGTATGA TTATCAAGAG
    AAGAGCCCTA GGGAGGTGAC AATGAAGAAA GGAGACATCT TGACCTTGCT AAACAGCACA
    AATAAGGATT GGTGGAAGGT GGAAGTGAAT GATCGTCAGG GCTTTGTACC TGCTGCGTAT
    GTGAAAAAGC TGGACCCAGC ACAGTCCGCA TCCCGGGAAA ACTTGCTGGA GGAACAGGGA
    AGCATCGCTC TCCGTCAGGA GCAGATCGAT GGCCAGTATC ATAATATTTT GGAGATGGGA
    GAGAAGCGCA AAGATATGTT AGAAAAGAGC TGCAAGAAGT TTATGCTGTT CCGTGAGGCA
    AATGAGCTGC AGCAGTGGAT TAACGAGAAA GAAGCATCAC TCACTAACGA AGAGGTCGGG
    GCTGACCTGG AACAAGTGGA AGTGCTGCAG AAGAAGTTTG ATGATTTCCA GAAGGATCTG
    AAAGCAAATG AGTCACGCTT GAAGGACATC AACAAGGTGG CCGACGAGCT TGAATCTGAA
    GGCCTCATTA CAGAGGAAGT CCAGCCGGTG CAGCATCAGG AGGTGTATGG GATGCAGCGG
    GATGAAGCAG ACTCCAAATC GGCATCTCCA TGGAAGTCTG TTCGCACAGC TGTGCTCGCG
    GTGGCCACCT TCAACTCTAT CAAGGACTTG AACGAGCGCT GGAAATCACT TCAACAGCTG
    GCAGAAGAAA GGAGTCAGCT TCTTGGCAGT GCCCATGAAG TACAGAGATT CCACAGAGAT
    GCTGATGAGA CAAAAGAATG GATCGAAGAG AAGAACCAGG CTTTGAATAC AGACAGCTAT
    GGACATGACT TGGCCAGTGT GCAGGCTCTG CAACGAAAAC ATGAAGGCTT TGAGAGAGAC
    CTCGCCGCTC TGGGAGATAA GGTGAACTCT TTGGGAGAAA CTGCTGAGCG CCTGATCCAG
    TCACACCCTG AGTCTGCAGA TGACATTCAG GAAAAATGCA CAGAGCTGAA TCAGGCCTGG
    AACAGCCTTG GCACCCGGGC AAACCAGCGC AAGGAGAAAC TAGGCGATTC TCATGATCTG
    CAACGCTTCC TAAGTGACTT CAGAGATCTC ATGTCCTGGA TCAATGGAAT TCGGGGCTTG
    GTGTCATCAG ATGAACTTGC TAGAGATGTG ACTGGTGCAG AGGCTTTGCT GGAACGACAC
    CAGGAGCACA GGACTGAGAT TGATGCCAGA GCAGGCACTT TTCAGGCCTT TGAGCAGTTT
    GGGCAGCAAC TTCTGGCTCG TGGTCATTAT GATAGTCCTG AAATCAAGGA GAAGCTGGAT
    ATCTTGGAAC AGGAACGGGC AAGTTTGGAA AAGGCTTGGG TGGAACGCAG AATGATGTTG
    GATCAGTGCT TGGAGCTGCA GCTCTTCAAC AGAGACTGCG AGCAGGCAGA GAACTGGATG
    GCAGCCCGTG AAGCCTTCCT GAACAGTGAG GATAAAGGGG ATTCCCTGGA CAGTGTGGAG
    GCCCTTATCA AGAAACATGA AGACTTTGAT AAAGCCATTA ATGTCCAGGA AGAGAAGATT
    GCTGCCCTCC AGGCCTTCGC CGAACAATTG ATAATTGCTG ACCACTATGC CAAAGGAGAT
    ATTGCCTCCC GTCGCAATGA AGTCCTGGAC AGGTGGCGCC GTCTCAAGGC TCAGATGATT
    GAGAAGAGAT CCAAACTTGG GGAATCTCAG ACTCTGCAGC AGTTCAGCAG GGACGTCGAT
    GAGATCGAAG CCTGGATCAG CGAGAAATTG CAGACCGCCA GTGACGAGTC CTACAAAGAC
    CCCACAAACA TCCAGCTTTC CAAATTGCTG AGCAAACACC AGAAGCACCA GGCTTTCGAA
    GCTGAGCTCC ATGCCAACGC TGACCGTATT CGTGGAGTAG TAGATATGGG GAACTCCCTT
    ATCGAACGAG GTGCCTGTGC TGGCAGCGAG GATGCGGTGA AGGCTCGCCT AAGCGCACTC
    GCTGATCAAT GGCAGTTCCT TGTCAGCAAA TCCGCAGAGA AGAGCCAGAA ACTGAAGGAG
    GCAAACAAGC AGCAGAACTT TAACACTGGC ATCAAAGACT TTGATTTCTG GTTAACAGAG
    GTGGAGGCTT TGCTGGCATC TGAAGACTAT GGGAAGGACT TGGCATCTGT TAACAACCTG
    CTGAAGAAAC ACCAGCTTCT GGAAGCTGAC ATCTCTGCTC ATGAGGACCG CCTTAAAGAT
    CTTAATGGGC AGGCTGATAG CCTGATGACC AGCAGCGCCT TTGACACCAC CCAAGTAAAG
    GAGAAAAGGG ACGCCATCAA CGACCGCTTC CAGCGTATCA AAAGCATGGC CACCGCACGC
    CGCAGCAGGC TGAATGAGTC CCACCGTCTG CACCAGTTCT TCAGAGATCT TGATGATGAG
    GAATCATGGA TCAAGGAAAA GAAACTTTTG GTCGGATCTG ATGACTATGG ACGTGACTTG
    ACTGGAGTGC AAAATCTGAG GAAGAAACAC AAGAGATTAG AAGCTGAACT GGCAGCTCAT
    GAGCCAGCCA TTCAGGGTGT GCTGGACACT GGGAAGAAGC TTGCAGATGA CAACACTATT
    GGGAAAGATG AGATTCAGCA GCGACTGGCA CAGTTTCTAG AGCACTGGAA GGAGCTGAAG
    GAACAAGCAG CGGCTCGTGG TCAGAGACTG GAGGAATCTC TTGAGTACCA ACAATTTGTA
    GCCAATGTGG AGGAAGAGGA AGCCTGGATT AATGAGAAGA TGACTCTAGT GGCCAGTGAA
    GACTATGGTG ACACACTGGC TGCCATTCAG GGATTGCTGA AAAAGCACGA GGCATTTGAG
    ACAGACTTTA CAGTGCACAA AGACAGAGTA CATGATGTAT GTGCCAACGG GGAAGACCTG
    ATACAAAAGA ACAATCACCA TGTGGAGAAT ATTAGCGCCA AAATGAAGGG TCTCCGAGGG
    AAGGTGGCAG AACTAGAGAA GGCGGCTGCA CAGCGCAAGG CCAAACTTGA TGAGAATTCA
    GCTTTCCTGC AGTTCAACTG GAAGGCGGAT GTTGTGGAGT CCTGGATCGG TGAAAAAGAG
    AACAGCCTGA AGACTGATGA TTATGGCAGA GACCTGTCCT CTGTGCAGAC CCTTCTCACT
    AAGCAGGAAA CATTTGATGC TGGGCTGCAA GCTTTCCAGC AAGAGGGCAT TACCAACATC
    ACTGCACTGA AAGACCAGCT TCTGGCTGCA AAGCATGTGC AGTCCCGGGC CATAGAGTCT
    CGTCACGCTT CTCTGATGAA GCGGTGGAAC CAGTTGTTGG ATAACTCTGC TGCCCGCAAA
    AAGAAGTTAT TGGAGGCCCA GGAACACTAC AGAAAGGTTG AGGACTTGTT CCTAACATTT
    GCCAAGAAAG CCTCTGCCTT CAACAGCTGG TTTGAGAATG CCGAGGAGGA TTTGACTGAT
    CCTGTTCGCT GCAACTCCCT GGAGGAAATC AAGGCGCTTC GCGAGGCACA TGACGCCTTC
    CGCAATTCCC TCAGCTCCGC ACAGACAGAT TTCAACCAGC TGGCCGAACT GGACAGGCAA
    ATAAAGGGCT ACCGGGTAGC CTCCAATCCC TACACGTGGT TCACGATGGA GGCTTTGGAG
    GAGACATGGC GCAACCTGCA GAAAATCATT AAGGAACGAG AACTTGAGCT ACAAAAAGAA
    CAACGTAGGC AGGAAGAGAA CGATAAGCTC CGGCAAGAGT TTGCTCAGCA CGCCAATGCT
    TTCCACCAGT GGATTCAGGA GACAAGGACC TACCTTCTGG ACGGAATAGC CTATCGCAGA
    GTCATACGTG TGTATCAGTA TGAAGTTGGG GATGATCTTT CTGGAAGGTC CTGTATGGTG
    GAAGAATCTG GAACACTGGA GTCACAACTT GAAGCTACAA AGAGAAAGCA TCAGGAGATC
    CGGGCCATGA GGAGTCAACT GAAGAAGATA GAAGATCTTG GCGCAGCCAT GGAAGAGGCC
    CTGATCTTGG ATAATAAATA CACAGAACAC AGCACTGTTG GGCTGGCACA GCAATGGGAC
    CAACTGGATC AGCTAGGCAT GAGAATGCAA CACAACCTAG AGCAACAGAT CCAGGCCAGA
    AACACAACTG GGGTCACAGA GGAAGCACTC AAAGAATTCA GCATGATGTT CAAGCATTTC
    GACAAAGACA AGTCTGGCCG CCTCAACCAC CAAGAATTCA AATCTTGTCT GCGGTCCCTT
    GGCTATGATC TGCCCATGGT AGAAGAAGGA GAACCAGACC CAGAGTTTGA AGCCATTTTG
    GACACTGTAG ATCCCAACAG AGATGGGCAC GTTTCTCTAC AGGAGTACAT GGCTTTCATG
    ATTAGCAGAG AAACTGAAAA CGTCAAGTCC AGTGAGGAGA TAGAGAGCGC CTTCCGAGCA
    CTGAGTACAG ACCAGAAACC TTATGTCACA AAGGAAGAAC TATACCAGAA CCTGACACGC
    GAACAGGCAG ATTACTGCAT CTCGCATATG AAACCCTACA TGGACAGCAA GGGCCGCGAA
    CTGCCCTCTG CATACGACTA CGTGGAATTC ACGCGCTCGC TATTTGTGAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031762664.1
    CDS4..7437
    Translation

    Target ORF information:

    RefSeq Version XM_031906804.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis spectrin, alpha, non-erythrocytic 1 (sptan1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031906804.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGATTCAG GTGGCGTGAA AGTGCTGGAG ACAGCGGAGG ACATCCAGGA AAGGCGCCAG 
    CAAGTCCTAG ACCGATACCT GCGGTTTAAG GAACTCTCTG TACTGAGACG TCAGAAGTTG
    GAGGATTCTT ACCGCTTCCA GTTCTTCCAG CGAGATGCTG ATGAACTGGA GAAATGGATC
    CAGGAGAAAC TCCAGATCGC ATCTGATGAG AACTACAAAG ATCCAACCAA TCTGCAGGGC
    AAATTGCAGA AACACCAGGC TTTTGAGGCA GAAGTACAGG CCAATGCAGG GGCAATTGTC
    AAACTGGACG AAACTGGAAA CCTGATGATT TCAGAAGGAC ATTTTTCATC AGAGACCATC
    AGAACCCGCC TGGTAGAGTT GCATCGTCTG TGGGAGCTGC TGCTCTTAAA GATGCAAGAG
    AAAGGGGTTA AATTACAACA GGCGCAGAAG CTTGTTCAGT ATCTACGGGA ATGTGAAGAT
    GTCCTAGACT GGATTAATGA CAAGGAGGCT ATTGTAACAT CTGAGGAGCT TGGTCAGGAT
    TTGGAACATG TGGAGGTCTT ACAGAAGAAG TTTGAAGAGT TTCAGACTGA TCTGGCAGCT
    CATGAAGAAA GAGTGAATGA AGTCAACCAG TTTGCAAGTA AACTGGCCCA GGAGGAACAC
    CCGGAGCTGG AGCTAATAAA GACCAAGCAG GATGAAGTCA ATGCAAGTTG GCAGCGCCTC
    AAAGGGCTGG CACTTCAGAG ACAAGGGAAG TTATTTGGAG CTGCAGAAGT TCAGCGTTTT
    AACAGAGATG TTGATGAAAC CATTGGATGG ATAAGAGAGA AGGAGCAGCT GATGGCATCT
    GATGACTTTG GCCGAGACCT GGCTAGCGTT CAAGCTCTCC TACGTAAACA CGAGGGTCTG
    GAGAGAGACC TTGCAGCACT GGAGGAAAAG GTAAAGGCCT TGTGTGCAGA ATCAGACCGT
    CTGCAGCAAT CCCATCCCAT GAACGCCCCT CAAATTCAAG TGAAAGGAGA AGAACTGATA
    ACCAACTGGG AGCAAATACG CACATTGGCA GCAGAACGAC ATGCTCGACT CAATGACTCT
    TACAAGCTTC AGCGTTTCCT TGCAGATTTT AGAGATCTGA CTAGCTGGGT GACTGAAATG
    AAAGCTCTGA TTAATGCCGA TGAGCTTGCC AACGATGTGG CTGGCGCAGA GGCTCTGCTT
    GACCGACACC AGGAACATAA GGGGGAAATT GATGCTCATG AAGATAGCTT TAAAGCTGCA
    GATGCTGCAG GTCAGGCCCT TCTGAACGGT GGCCACTACG CCTCAGATGA AGTCCGAGAA
    AAGCTTACTA TCTTAGCAGA AGAAAGGGCA GCTCTTCTAG AGCTTTGGGA GCTCCGTCGT
    CAGCAGTATG AACAGTGCAT GGACCTGCAG CTTTTTTACA GAGACACCGA ACAGGTGGAC
    AACTGGATGA GCAAACAGGA AGCTTTCCTG CTTAACGAGG ACTTGGGTGA TTCTCTAGAC
    AGTGTGGAAG CTCTTCTGAA AAAACATGAG GACTTTGAGA AGTCTCTTAG CGCCCAGGAA
    GAGAAAATCA CAGCTCTGGA TGAGTTTGCT ACAAAACTAA TTCAAAATAA CCACTATGCC
    AAGGAAGATG TGGCTACCCG CAGAGATGCT CTGCTGAGCC GCCGCAATGC ACTTCATGAA
    CGTGCACTCT ACCGCCGTAC TCAACTGGCA GACTCCTTTC ACCTGCAGCA GTTCTTTAGG
    GACTCTGACG AGCTGAAAAG TTGGATTAAT GAAAAGATGA AGACTGCAAC GGATGAGGCA
    TACAAGGATC CCTCCAACCT CCAGGGTAAG GTGCAGAAAC ACCAAGCTTT TGAGGCTGAA
    CTCTCCGCTA ACCAAAGCCG TATAGATGCC CTGGAGAATT CTGGGCAGAA ATTGATTGAT
    GTCAACCATT ATGCATCAGA TGAGGTGGCA GCACGTATGA GTGAGGTCAT CACTTTGTGG
    AAGAAGCTGC TGGAGGCCAC TGAATTGAAG GGGATCAAGC TGCGCGAAGC CAACCAGCAG
    CAGCAGTTCA ACCGTAATGT GGAGGATATA GAGCTGTGGC TGTATGAGGT TGAGGGGCAT
    TTGGCTTCAG ATGACTATGG AAAAGACCTG ACCAATGTTC AAAACTTGCA GAAAAAACAC
    GCCTTGCTGG AGGCTGATGT TGCAGCTCAC CAGGACCGTA TCGATGGCAT CACTATCCAA
    GCCCGTCAGT TCCAAGAAGC TGGTCATTTT GATGCTGACA ATATCAGGAA GAAACAGGAG
    GCCCTTGTTA ATAGATATGA AGCTTTGAAA GAACCCATGG TTGCACGCAA GCAGAAGCTG
    TCAGACTCTC TGCGTCTTCA ACAGCTTTTC AGGGATGTTG AAGATGAGGA AACCTGGATA
    AGGGAGAAGG AACCCATTGC AGCGTCCACC AACAGAGGCA AGGATTTGAT TGGAGTCCAG
    AATCTCCTGA AGAAACACCA GGCTTTGCAG GCTGAAATTG CAGGGCACGA ACCACGAATT
    AAAGCTGTTA CCCAGAAAGG AGAATCTATG ATCTCTGAAG GCCACTTTGC TTCTGATGAA
    GTGAAGGGCA AATTAAGGGA GCTGAATGAT AAGTGGGCCA CCCTGAAGAA CAAAGCATCT
    CAGCGCCGAC AGGACCTTGA AGATTCTCTG CAAGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCAGAGT CCTGGATGAG AGAGAAGGAA CCTATTGTTG GAAGCACCGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC CCTTCTGAAG AAACATGAAG CGCTTATGTC AGATCTGCGA
    GCATACGGAA GCAGTATTCA GGGACTTAGA GAGCAGGCCC AGGCCTGCAG GCAACAAGTT
    GCTCCAACTG ATGATGAAAC TGGAAAGGAG CTGGTGCTGG CATTGTATGA TTATCAAGAG
    AAGAGCCCTA GGGAGGTGAC AATGAAGAAA GGAGACATCT TGACCTTGCT AAACAGCACA
    AATAAGGATT GGTGGAAGGT GGAAGTGAAT GATCGTCAGG GCTTTGTACC TGCTGCGTAT
    GTGAAAAAGC TGGACCCAGC ACAGTCCGCA TCCCGGGAAA ACTTGCTGGA GGAACAGGGA
    AGCATCGCTC TCCGTCAGGA GCAGATCGAT GGCCAGTATC ATAATATTTT GGAGATGGGA
    GAGAAGCGCA AAGATATGTT AGAAAAGAGC TGCAAGAAGT TTATGCTGTT CCGTGAGGCA
    AATGAGCTGC AGCAGTGGAT TAACGAGAAA GAAGCATCAC TCACTAACGA AGAGGTCGGG
    GCTGACCTGG AACAAGTGGA AGTGCTGCAG AAGAAGTTTG ATGATTTCCA GAAGGATCTG
    AAAGCAAATG AGTCACGCTT GAAGGACATC AACAAGGTGG CCGACGAGCT TGAATCTGAA
    GGCCTCATTA CAGAGGAAGT CCAGCCGGTG CAGCATCAGG AGGTGTATGG GATGCAGCGG
    GATGAAGCAG ACTCCAAATC GGCATCTCCA TGGAAGTCTG TTCGCACAGC TGTGCTCGCG
    GTGGCCACCT TCAACTCTAT CAAGGACTTG AACGAGCGCT GGAAATCACT TCAACAGCTG
    GCAGAAGAAA GGAGTCAGCT TCTTGGCAGT GCCCATGAAG TACAGAGATT CCACAGAGAT
    GCTGATGAGA CAAAAGAATG GATCGAAGAG AAGAACCAGG CTTTGAATAC AGACAGCTAT
    GGACATGACT TGGCCAGTGT GCAGGCTCTG CAACGAAAAC ATGAAGGCTT TGAGAGAGAC
    CTCGCCGCTC TGGGAGATAA GGTGAACTCT TTGGGAGAAA CTGCTGAGCG CCTGATCCAG
    TCACACCCTG AGTCTGCAGA TGACATTCAG GAAAAATGCA CAGAGCTGAA TCAGGCCTGG
    AACAGCCTTG GCACCCGGGC AAACCAGCGC AAGGAGAAAC TAGGCGATTC TCATGATCTG
    CAACGCTTCC TAAGTGACTT CAGAGATCTC ATGTCCTGGA TCAATGGAAT TCGGGGCTTG
    GTGTCATCAG ATGAACTTGC TAGAGATGTG ACTGGTGCAG AGGCTTTGCT GGAACGACAC
    CAGGAGCACA GGACTGAGAT TGATGCCAGA GCAGGCACTT TTCAGGCCTT TGAGCAGTTT
    GGGCAGCAAC TTCTGGCTCG TGGTCATTAT GATAGTCCTG AAATCAAGGA GAAGCTGGAT
    ATCTTGGAAC AGGAACGGGC AAGTTTGGAA AAGGCTTGGG TGGAACGCAG AATGATGTTG
    GATCAGTGCT TGGAGCTGCA GCTCTTCAAC AGAGACTGCG AGCAGGCAGA GAACTGGATG
    GCAGCCCGTG AAGCCTTCCT GAACAGTGAG GATAAAGGGG ATTCCCTGGA CAGTGTGGAG
    GCCCTTATCA AGAAACATGA AGACTTTGAT AAAGCCATTA ATGTCCAGGA AGAGAAGATT
    GCTGCCCTCC AGGCCTTCGC CGAACAATTG ATAATTGCTG ACCACTATGC CAAAGGAGAT
    ATTGCCTCCC GTCGCAATGA AGTCCTGGAC AGGTGGCGCC GTCTCAAGGC TCAGATGATT
    GAGAAGAGAT CCAAACTTGG GGAATCTCAG ACTCTGCAGC AGTTCAGCAG GGACGTCGAT
    GAGATCGAAG CCTGGATCAG CGAGAAATTG CAGACCGCCA GTGACGAGTC CTACAAAGAC
    CCCACAAACA TCCAGCTTTC CAAATTGCTG AGCAAACACC AGAAGCACCA GGCTTTCGAA
    GCTGAGCTCC ATGCCAACGC TGACCGTATT CGTGGAGTAG TAGATATGGG GAACTCCCTT
    ATCGAACGAG GTGCCTGTGC TGGCAGCGAG GATGCGGTGA AGGCTCGCCT AAGCGCACTC
    GCTGATCAAT GGCAGTTCCT TGTCAGCAAA TCCGCAGAGA AGAGCCAGAA ACTGAAGGAG
    GCAAACAAGC AGCAGAACTT TAACACTGGC ATCAAAGACT TTGATTTCTG GTTAACAGAG
    GTGGAGGCTT TGCTGGCATC TGAAGACTAT GGGAAGGACT TGGCATCTGT TAACAACCTG
    CTGAAGAAAC ACCAGCTTCT GGAAGCTGAC ATCTCTGCTC ATGAGGACCG CCTTAAAGAT
    CTTAATGGGC AGGCTGATAG CCTGATGACC AGCAGCGCCT TTGACACCAC CCAAGTAAAG
    GAGAAAAGGG ACGCCATCAA CGACCGCTTC CAGCGTATCA AAAGCATGGC CACCGCACGC
    CGCAGCAGGC TGAATGAGTC CCACCGTCTG CACCAGTTCT TCAGAGATCT TGATGATGAG
    GAATCATGGA TCAAGGAAAA GAAACTTTTG GTCGGATCTG ATGACTATGG ACGTGACTTG
    ACTGGAGTGC AAAATCTGAG GAAGAAACAC AAGAGATTAG AAGCTGAACT GGCAGCTCAT
    GAGCCAGCCA TTCAGGGTGT GCTGGACACT GGGAAGAAGC TTGCAGATGA CAACACTATT
    GGGAAAGATG AGATTCAGCA GCGACTGGCA CAGTTTCTAG AGCACTGGAA GGAGCTGAAG
    GAACAAGCAG CGGCTCGTGG TCAGAGACTG GAGGAATCTC TTGAGTACCA ACAATTTGTA
    GCCAATGTGG AGGAAGAGGA AGCCTGGATT AATGAGAAGA TGACTCTAGT GGCCAGTGAA
    GACTATGGTG ACACACTGGC TGCCATTCAG GGATTGCTGA AAAAGCACGA GGCATTTGAG
    ACAGACTTTA CAGTGCACAA AGACAGAGTA CATGATGTAT GTGCCAACGG GGAAGACCTG
    ATACAAAAGA ACAATCACCA TGTGGAGAAT ATTAGCGCCA AAATGAAGGG TCTCCGAGGG
    AAGGTGGCAG AACTAGAGAA GGCGGCTGCA CAGCGCAAGG CCAAACTTGA TGAGAATTCA
    GCTTTCCTGC AGTTCAACTG GAAGGCGGAT GTTGTGGAGT CCTGGATCGG TGAAAAAGAG
    AACAGCCTGA AGACTGATGA TTATGGCAGA GACCTGTCCT CTGTGCAGAC CCTTCTCACT
    AAGCAGGAAA CATTTGATGC TGGGCTGCAA GCTTTCCAGC AAGAGGGCAT TACCAACATC
    ACTGCACTGA AAGACCAGCT TCTGGCTGCA AAGCATGTGC AGTCCCGGGC CATAGAGTCT
    CGTCACGCTT CTCTGATGAA GCGGTGGAAC CAGTTGTTGG ATAACTCTGC TGCCCGCAAA
    AAGAAGTTAT TGGAGGCCCA GGAACACTAC AGAAAGGTTG AGGACTTGTT CCTAACATTT
    GCCAAGAAAG CCTCTGCCTT CAACAGCTGG TTTGAGAATG CCGAGGAGGA TTTGACTGAT
    CCTGTTCGCT GCAACTCCCT GGAGGAAATC AAGGCGCTTC GCGAGGCACA TGACGCCTTC
    CGCAATTCCC TCAGCTCCGC ACAGACAGAT TTCAACCAGC TGGCCGAACT GGACAGGCAA
    ATAAAGGGCT ACCGGGTAGC CTCCAATCCC TACACGTGGT TCACGATGGA GGCTTTGGAG
    GAGACATGGC GCAACCTGCA GAAAATCATT AAGGAACGAG AACTTGAGCT ACAAAAAGAA
    CAACGTAGGC AGGAAGAGAA CGATAAGCTC CGGCAAGAGT TTGCTCAGCA CGCCAATGCT
    TTCCACCAGT GGATTCAGGA GACAAGGACC TACCTTCTGG ACGGAATAGC CTATCGCAGA
    GTCATACGTG TGTATCAGTA TGAAGTTGGG GATGATCTTT CTGGAAGGTC CTGTATGGTG
    GAAGAATCTG GAACACTGGA GTCACAACTT GAAGCTACAA AGAGAAAGCA TCAGGAGATC
    CGGGCCATGA GGAGTCAACT GAAGAAGATA GAAGATCTTG GCGCAGCCAT GGAAGAGGCC
    CTGATCTTGG ATAATAAATA CACAGAACAC AGCACTGTTG GGCTGGCACA GCAATGGGAC
    CAACTGGATC AGCTAGGCAT GAGAATGCAA CACAACCTAG AGCAACAGAT CCAGGCCAGA
    AACACAACTG GGGTCACAGA GGAAGCACTC AAAGAATTCA GCATGATGTT CAAGCATTTC
    GACAAAGACA AGTCTGGCCG CCTCAACCAC CAAGAATTCA AATCTTGTCT GCGGTCCCTT
    GGCTATGATC TGCCCATGGT AGAAGAAGGA GAACCAGACC CAGAGTTTGA AGCCATTTTG
    GACACTGTAG ATCCCAACAG AGATGGGCAC GTTTCTCTAC AGGAGTACAT GGCTTTCATG
    ATTAGCAGAG AAACTGAAAA CGTCAAGTCC AGTGAGGAGA TAGAGAGCGC CTTCCGAGCA
    CTGAGTACAG ACCAGAAACC TTATGTCACA AAGGAAGAAC TATACCAGAA CCTGACACGC
    GAACAGGCAG ATTACTGCAT CTCGCATATG AAACCCTACA TGGACAGCAA GGGCCGCGAA
    CTGCCCTCTG CATACGACTA CGTGGAATTC ACGCGCTCGC TATTTGTGAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa22512
    Clone ID Related Accession (Same CDS sequence) NM_001097205.1
    Accession Version NM_001097205.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7416bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-21
    Organism Xenopus tropicalis(tropical clawed frog)
    Product spectrin alpha chain, non-erythrocytic 1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC127322.1. ##Evidence-Data-START## Transcript exon combination :: BC127322.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGATTCAG GTGGCGTGAA AGTGCTGGAG ACAGCGGAGG ACATCCAGGA AAGGCGCCAG 
    CAAGTCCTAG ACCGATACCT GCGGTTTAAG GAACTCTCTG TACTGAGACG TCAGAAGTTG
    GAGGATTCTT ACCGCTTCCA GTTCTTCCAG CGAGATGCTG ATGAACTGGA GAAATGGATC
    CAGGAGAAAC TCCAGATCGC ATCTGATGAG AACTACAAAG ATCCAACCAA TCTGCAGGGC
    AAATTGCAGA AACACCAGGC TTTTGAGGCA GAAGTACAGG CCAATGCAGG GGCAATTGTC
    AAACTGGACG AAACTGGAAA CCTGATGATT TCAGAAGGAC ATTTTTCATC AGAGACCATC
    AGAACCCGCC TGGTAGAGTT GCATCGTCTG TGGGAGCTGC TGCTCTTAAA GATGCAAGAG
    AAAGGGGTTA AATTACAACA GGCGCAGAAG CTTGTTCAGT ATCTACGGGA ATGTGAAGAT
    GTCCTAGACT GGATTAATGA CAAGGAGGCT ATTGTAACAT CTGAGGAGCT TGGTCAGGAT
    TTGGAACATG TGGAGGTCTT ACAGAAGAAG TTTGAAGAGT TTCAGACTGA TCTGGCAGCT
    CATGAAGAAA GAGTGAATGA AGTCAACCAG TTTGCAAGTA AACTGGCCCA GGAGGAACAC
    CCGGAGCTGG AGCTAATAAA GACCAAGCAG GATGAAGTCA ATGCAAGTTG GCAGCGCCTC
    AAAGGGCTGG CACTTCAGAG ACAAGGGAAG TTATTTGGAG CTGCAGAAGT TCAGCGTTTT
    AACAGAGATG TTGATGAAAC CATTGGATGG ATAAGAGAGA AGGAGCAGCT GATGGCATCT
    GATGACTTTG GCCGAGACCT GGCTAGCGTT CAAGCTCTCC TACGTAAACA CGAGGGTCTG
    GAGAGAGACC TTGCAGCACT GGAGGAAAAG GTAAAGGCCT TGTGTGCAGA ATCAGACCGT
    CTGCAGCAAT CCCATCCCAT GAACGCCCCT CAAATTCAAG TGAAAGGAGA AGAACTGATA
    ACCAACTGGG AGCAAATACG CACATTGGCA GCAGAACGAC ATGCTCGACT CAATGACTCT
    TACAAGCTTC AGCGTTTCCT TGCAGATTTT AGAGATCTGA CTAGCTGGGT GACTGAAATG
    AAAGCTCTGA TTAATGCCGA TGAGCTTGCC AACGATGTGG CTGGCGCAGA GGCTCTGCTT
    GACCGACACC AGGAACATAA GGGGGAAATT GATGCTCATG AAGATAGCTT TAAAGCTGCA
    GATGCTGCAG GTCAGGCCCT TCTGAACGGT GGCCACTACG CCTCAGATGA AGTCCGAGAA
    AAGCTTACTA TCTTAGCAGA AGAAAGGGCA GCTCTTCTAG AGCTTTGGGA GCTCCGTCGT
    CAGCAGTATG AACAGTGCAT GGACCTGCAG CTTTTTTACA GAGACACCGA ACAGGTGGAC
    AACTGGATGA GCAAACAGGA AGCTTTCCTG CTTAACGAGG ACTTGGGTGA TTCTCTAGAC
    AGTGTGGAAG CTCTTCTGAA AAAACATGAG GACTTTGAGA AGTCTCTTAG CGCCCAGGAA
    GAGAAAATCA CAGCTCTGGA TGAGTTTGCT ACAAAACTAA TTCAAAATAA CCACTATGCC
    AAGGAAGATG TGGCTACCCG CAGAGATGCT CTGCTGAGCC GCCGCAATGC ACTTCATGAA
    CGTGCACTCT ACCGCCGTAC TCAACTGGCA GACTCCTTTC ACCTGCAGCA GTTCTTTAGG
    GACTCTGACG AGCTGAAAAG TTGGATTAAT GAAAAGATGA AGACTGCAAC GGATGAGGCA
    TACAAGGATC CCTCCAACCT CCAGGGTAAG GTGCAGAAAC ACCAAGCTTT TGAGGCTGAA
    CTCTCCGCTA ACCAAAGCCG TATAGATGCC CTGGAGAATT CTGGGCAGAA ATTGATTGAT
    GTCAACCATT ATGCATCAGA TGAGGTGGCA GCACGTATGA GTGAGGTCAT CACTTTGTGG
    AAGAAGCTGC TGGAGGCCAC TGAATTGAAG GGGATCAAGC TGCGCGAAGC CAACCAGCAG
    CAGCAGTTCA ACCGTAATGT GGAGGATATA GAGCTGTGGC TGTATGAGGT TGAGGGGCAT
    TTGGCTTCAG ATGACTATGG AAAAGACCTG ACCAATGTTC AAAACTTGCA GAAAAAACAC
    GCCTTGCTGG AGGCTGATGT TGCAGCTCAC CAGGACCGTA TCGATGGCAT CACTATCCAA
    GCCCGTCAGT TCCAAGAAGC TGGTCATTTT GATGCTGACA ATATCAGGAA GAAACAGGAG
    GCCCTTGTTA ATAGATATGA AGCTTTGAAA GAACCCATGG TTGCACGCAA GCAGAAGCTG
    TCAGACTCTC TGCGTCTTCA ACAGCTTTTC AGGGATGTTG AAGATGAGGA AACCTGGATA
    AGGGAGAAGG AACCCATTGC AGCGTCCACC AACAGAGGCA AGGATTTGAT TGGAGTCCAG
    AATCTCCTGA AGAAACACCA GGCTTTGCAG GCTGAAATTG CAGGGCACGA ACCACGAATT
    AAAGCTGTTA CCCAGAAAGG AGAATCTATG ATCTCTGAAG GCCACTTTGC TTCTGATGAA
    GTGAAGGGCA AATTAAGGGA GCTGAATGAT AAGTGGGCCA CCCTGAAGAA CAAAGCATCT
    CAGCGCCGAC AGGACCTTGA AGATTCTCTG CAAGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCAGAGT CCTGGATGAG AGAGAAGGAA CCTATTGTTG GAAGCACCGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC CCTTCTGAAG AAACATGAAG CGCTTATGTC AGATCTGCGA
    GCATACGGAA GCAGTATTCA GGGACTTAGA GAGCAGGCCC AGGCCTGCAG GCAACAAGTT
    GCTCCAACTG ATGATGAAAC TGGAAAGGAG CTGGTGCTGG CATTGTATGA TTATCAAGAG
    AAGAGCCCTA GGGAGGTGAC AATGAAGAAA GGAGACATCT TGACCTTGCT AAACAGCACA
    AATAAGGATT GGTGGAAGGT GGAAGTGAAT GATCGTCAGG GCTTTGTACC TGCTGCGTAT
    GTGAAAAAGC TGGACCCAGC ACAGTCCGCA TCCCGGGAAA ACTTGCTGGA GGAACAGGGA
    AGCATCGCTC TCCGTCAGGA GCAGATCGAT GGCCAGGCTC ACATGTCTAA GGAAGCTGGC
    AGTGTTTCTC TGCGTATGAA TCAGGTTGAA GAATTGTATC ATAATATTTT GGAGATGGGA
    GAGAAGCGCA AAGATATGTT AGAAAAGAGC TGCAAGAAGT TTATGCTGTT CCGTGAGGCA
    AATGAGCTGC AGCAGTGGAT TAACGAGAAA GAAGCATCAC TCACTAACGA AGAGGTCGGG
    GCTGACCTGG AACAAGTGGA AGTGCTGCAG AAGAAGTTTG ATGATTTCCA GAAGGATCTG
    AAAGCAAATG AGTCACGCTT GAAGGACATC AACAAGGTGG CCGACGAGCT TGAATCTGAA
    GGCCTCATTA CAGAGGAAGT CCAGCCGGTG CAGCATCAGG AGGTGTATGG GATGCAGCGG
    GATGAAGCAG ACTCCAAATC GGCATCTCCA TGGAAGTCTG TTCGCACAGC TGTGCTCGCG
    GTGGCCACCT TCAACTCTAT CAAGGACTTG AACGAGCGCT GGAAATCACT TCAACAGCTG
    GCAGAAGAAA GGAGTCAGCT TCTTGGCAGT GCCCATGAAG TACAGAGATT CCACAGAGAT
    GCTGATGAGA CAAAAGAATG GATCGAAGAG AAGAACCAGG CTTTGAATAC AGACAGCTAT
    GGACATGACT TGGCCAGTGT GCAGGCTCTG CAACGAAAAC ATGAAGGTTT TGAGAGAGAC
    CTCGCCGCTC TGGGAGATAA GGTGAACTCT TTGGGAGAAA CTGCTGAGCG CCTGATCCAG
    TCACACCCTG AGTCTGCAGA TGACATTCAG GAAAAATGCA CAGAGCTGAA TCAGGCCTGG
    AACAGCCTTG GCACCCGGGC AAACCAGCGC AAGGAGAAAC TAGGCGATTC TCATGATCTG
    CAACGCTTCC TAAGTGACTT CAGAGATCTC ATGTCCTGGA TCAATGGAAT TCGGGGCTTG
    GTGTCATCAG ATGAACTTGC TAGAGATGTG ACTGGTGCAG AGGCTTTGCT GGAACGACAC
    CAGGAGCACA GGACTGAGAT TGATGCCAGA GCAGGCACTT TTCAGGCCTT TGAGCAGTTT
    GGGCAGCAAC TTCTGGCTCG TGGTCATTAT GATAGTCCTG AAATCAAGGA GAAGCTGGAT
    ATCTTGGAAC AGGAACGGGC AAGTTTGGAA AAGGCTTGGG TGGAACGCAG AATGATGTTG
    GATCAGTGCT TGGAGCTGCA GCTCTTCAAC AGAGACTGCG AGCAGGCAGA GAACTGGATG
    GCAGCCCGTG AAGCCTTCCT GAACAGTGAG GATAAAGGGG ATTCCCTGGA CAGTGTGGAG
    GCCCTTATCA AGAAACATGA AGACTTTGAT AAAGCCATTA ATGTCCAGGA AGAGAAGATT
    GCTGCCCTCC AGGCCTTCGC CGAACAATTG ATAATTGCTG ACCACTATGC CAAAGGAGAT
    ATTGCCTCCC GTCGCAATGA AGTCCTGGAC AGGTGGCGCC GTCTCAAGGC TCAGATGATT
    GAGAAGAGAT CCAAACTTGG GGAATCTCAG ACTCTGCAGC AGTTCAGCAG GGACGTCGAT
    GAGATCGAAG CCTGGATCAG CGAGAAATTG CAGACCGCCA GTGACGAGTC CTACAAAGAC
    CCCACAAACA TCCAGAGCAA ACACCAGAAG CACCAGGCTT TCGAAGCTGA GCTCCATGCC
    AACGCTGACC GTATTCGTGG AGTAGTAGAT ATGGGGAACT CCCTTATCGA ACGAGGTGCC
    TGTGCTGGCA GCGAGGATGC GGTGAAGGCT CGCCTAAGCG CACTCGCTGA TCAATGGCAG
    TTCCTTGTCA GCAAATCCGC AGAGAAGAGC CAGAAACTGA AGGAGGCAAA CAAGCAGCAG
    AACTTTAACA CTGGCATCAA AGACTTTGAT TTCTGGTTAA CAGAGGTGGA GGCTTTGCTG
    GCATCTGAAG ACTATGGGAA GGACTTGGCA TCTGTTAACA ACCTGCTGAA GAAACACCAG
    CTTCTGGAAG CTGACATCTC TGCTCATGAG GACCGCCTTA AAGATCTTAA TGGGCAGGCT
    GATAGCCTGA TGACCAGCAG CGCCTTTGAC ACCACCCAAG TAAAGGAGAA AAGGGACGCC
    ATCAACGACC GCTTCCAGCG TATCAAAAGC ATGGCCACCG CACGCCGCAG CAGGCTGAAT
    GAGTCCCACC GTCTGCACCA GTTCTTCAGA GATCTTGATG ATGAGGAATC ATGGATCAAG
    GAAAAGAAAC TTTTGGTCGG ATCTGATGAC TATGGACGTG ACTTGACTGG AGTGCAAAAT
    CTGAGGAAGA AACACAAGAG ATTAGAAGCT GAACTGGCAG CTCATGAGCC AGCCATTCAG
    GGTGTGCTGG ACACTGGGAA GAAGCTTGCA GATGACAACA CTATTGGGAA AGATGAGATT
    CAGCAGCGAC TGGCACAGTT TCTAGAGCAC TGGAAGGAGC TGAAGGAACA AGCAGCGGCT
    CGTGGTCAGA GACTGGAGGA ATCTCTTGAG TACCAACAAT TTGTAGCCAA TGTGGAGGAA
    GAGGAAGCCT GGATTAATGA GAAGATGACT CTAGTGGCCA GTGAAGACTA TGGTGACACA
    CTGGCTGCCA TTCAGGGATT GCTGAAAAAG CACGAGGCAT TTGAGACAGA CTTTACAGTG
    CACAAAGACA GAGTACATGA TGTATGTGCC AACGGGGAAG ACCTGATACA AAAGAACAAT
    CACCATGTGG AGAATATTAG CGCCAAAATG AAGGGTCTCC GAGGGAAGGT GGCAGAACTA
    GAGAAGGCGG CTGCACAGCG CAAGGCCAAA CTTGATGAGA ATTCAGCTTT CCTGCAGTTC
    AACTGGAAGG CGGATGTTGT GGAGTCCTGG ATCGGTGAAA AAGAGAACAG CCTGAAGACT
    GATGATTATG GCAGAGACCT GTCCTCTGTG CAGACCCTTC TCACTAAGCA GGAAACATTT
    GATGCTGGGC TGCAAGCTTT CCAGCAAGAG GGCATTACCA ACATCACTGC ACTGAAAGAC
    CAGCTTCTGG CTGCAAAGCA TGTGCAGTCC CGGGCCATAG AGTCTCGTCA CGCTTCTCTG
    ATGAAGCGGT GGAACCAGTT GTTGGATAAC TCTGCTGCCC GCAAAAAGAA GTTATTGGAG
    GCCCAGGAAC ACTACAGAAA GGTTGAGGAC TTGTTCCTAA CATTTGCCAA GAAAGCCTCT
    GCCTTCAACA GCTGGTTTGA GAATGCCGAG GAGGATTTGA CTGATCCTGT TCGCTGCAAC
    TCCCTGGAGG AAATCAAGGC GCTTCGCGAG GCACATGACG CCTTCCGCAA TTCCCTCAGC
    TCCGCACAGA CAGATTTCAA CCAGCTGGCC GAACTGGACA GGCAAATAAA GGGCTACCGG
    GTAGCCTCCA ATCCCTACAC GTGGTTCACG ATGGAGGCTT TGGAGGAGAC ATGGCGCAAC
    CTGCAGAAAA TCATTAAGGA ACGAGAACTT GAGCTACAAA AAGAACAACG TAGGCAGGAA
    GAGAACGATA AGCTCCGGCA AGAGTTTGCT CAGCACGCCA ATGCTTTCCA CCAGTGGATT
    CAGGAGACAA GGACCTACCT TCTGGACGGG TCCTGTATGG TGGAAGAATC TGGAACACTG
    GAGTCACAAC TTGAAGCTAC AAAGAGAAAG CATCAGGAGA TCCGGGCCAT GAGGAGTCAA
    CTGAAGAAGA TAGAAGATCT TGGCGCAGCC ATGGAAGAGG CCCTGATCTT GGATAATAAA
    TACACAGAAC ACAGCACTGT TGGGCTGGCA CAGCAATGGG ACCAACTGGA TCAGCTAGGC
    ATGAGAATGC AACACAACCT AGAGCAACAG ATCCAGGCCA GAAACACAAC TGGGGTCACA
    GAGGAAGCAC TCAAAGAATT CAGCATGATG TTCAAGCATT TCGACAAAGA CAAGTCTGGC
    CGCCTCAACC ACCAAGAATT CAAATCTTGT CTGCGGTCCC TTGGCTATGA TCTGCCCATG
    GTAGAAGAAG GAGAACCAGA CCCAGAGTTT GAAGCCATTT TGGACACTGT AGATCCCAAC
    AGAGATGGGC ACGTTTCTCT ACAGGAGTAC ATGGCTTTCA TGATTAGCAG AGAAACTGAA
    AACGTCAAGT CCAGTGAGGA GATAGAGAGC GCCTTCCGAG CACTGAGTAC AGACCAGAAA
    CCTTATGTCA CAAAGGAAGA ACTATACCAG AACCTGACAC GCGAACAGGC AGATTACTGC
    ATCTCGCATA TGAAACCCTA CATGGACAGC AAGGGCCGCG AACTGCCCTC TGCATACGAC
    TACGTGGAAT TCACGCGCTC GCTATTTGTG AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001090674.1
    CDS137..7552
    Translation

    Target ORF information:

    RefSeq Version NM_001097205.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis spectrin, alpha, non-erythrocytic 1 (sptan1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001097205.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGATTCAG GTGGCGTGAA AGTGCTGGAG ACAGCGGAGG ACATCCAGGA AAGGCGCCAG 
    CAAGTCCTAG ACCGATACCT GCGGTTTAAG GAACTCTCTG TACTGAGACG TCAGAAGTTG
    GAGGATTCTT ACCGCTTCCA GTTCTTCCAG CGAGATGCTG ATGAACTGGA GAAATGGATC
    CAGGAGAAAC TCCAGATCGC ATCTGATGAG AACTACAAAG ATCCAACCAA TCTGCAGGGC
    AAATTGCAGA AACACCAGGC TTTTGAGGCA GAAGTACAGG CCAATGCAGG GGCAATTGTC
    AAACTGGACG AAACTGGAAA CCTGATGATT TCAGAAGGAC ATTTTTCATC AGAGACCATC
    AGAACCCGCC TGGTAGAGTT GCATCGTCTG TGGGAGCTGC TGCTCTTAAA GATGCAAGAG
    AAAGGGGTTA AATTACAACA GGCGCAGAAG CTTGTTCAGT ATCTACGGGA ATGTGAAGAT
    GTCCTAGACT GGATTAATGA CAAGGAGGCT ATTGTAACAT CTGAGGAGCT TGGTCAGGAT
    TTGGAACATG TGGAGGTCTT ACAGAAGAAG TTTGAAGAGT TTCAGACTGA TCTGGCAGCT
    CATGAAGAAA GAGTGAATGA AGTCAACCAG TTTGCAAGTA AACTGGCCCA GGAGGAACAC
    CCGGAGCTGG AGCTAATAAA GACCAAGCAG GATGAAGTCA ATGCAAGTTG GCAGCGCCTC
    AAAGGGCTGG CACTTCAGAG ACAAGGGAAG TTATTTGGAG CTGCAGAAGT TCAGCGTTTT
    AACAGAGATG TTGATGAAAC CATTGGATGG ATAAGAGAGA AGGAGCAGCT GATGGCATCT
    GATGACTTTG GCCGAGACCT GGCTAGCGTT CAAGCTCTCC TACGTAAACA CGAGGGTCTG
    GAGAGAGACC TTGCAGCACT GGAGGAAAAG GTAAAGGCCT TGTGTGCAGA ATCAGACCGT
    CTGCAGCAAT CCCATCCCAT GAACGCCCCT CAAATTCAAG TGAAAGGAGA AGAACTGATA
    ACCAACTGGG AGCAAATACG CACATTGGCA GCAGAACGAC ATGCTCGACT CAATGACTCT
    TACAAGCTTC AGCGTTTCCT TGCAGATTTT AGAGATCTGA CTAGCTGGGT GACTGAAATG
    AAAGCTCTGA TTAATGCCGA TGAGCTTGCC AACGATGTGG CTGGCGCAGA GGCTCTGCTT
    GACCGACACC AGGAACATAA GGGGGAAATT GATGCTCATG AAGATAGCTT TAAAGCTGCA
    GATGCTGCAG GTCAGGCCCT TCTGAACGGT GGCCACTACG CCTCAGATGA AGTCCGAGAA
    AAGCTTACTA TCTTAGCAGA AGAAAGGGCA GCTCTTCTAG AGCTTTGGGA GCTCCGTCGT
    CAGCAGTATG AACAGTGCAT GGACCTGCAG CTTTTTTACA GAGACACCGA ACAGGTGGAC
    AACTGGATGA GCAAACAGGA AGCTTTCCTG CTTAACGAGG ACTTGGGTGA TTCTCTAGAC
    AGTGTGGAAG CTCTTCTGAA AAAACATGAG GACTTTGAGA AGTCTCTTAG CGCCCAGGAA
    GAGAAAATCA CAGCTCTGGA TGAGTTTGCT ACAAAACTAA TTCAAAATAA CCACTATGCC
    AAGGAAGATG TGGCTACCCG CAGAGATGCT CTGCTGAGCC GCCGCAATGC ACTTCATGAA
    CGTGCACTCT ACCGCCGTAC TCAACTGGCA GACTCCTTTC ACCTGCAGCA GTTCTTTAGG
    GACTCTGACG AGCTGAAAAG TTGGATTAAT GAAAAGATGA AGACTGCAAC GGATGAGGCA
    TACAAGGATC CCTCCAACCT CCAGGGTAAG GTGCAGAAAC ACCAAGCTTT TGAGGCTGAA
    CTCTCCGCTA ACCAAAGCCG TATAGATGCC CTGGAGAATT CTGGGCAGAA ATTGATTGAT
    GTCAACCATT ATGCATCAGA TGAGGTGGCA GCACGTATGA GTGAGGTCAT CACTTTGTGG
    AAGAAGCTGC TGGAGGCCAC TGAATTGAAG GGGATCAAGC TGCGCGAAGC CAACCAGCAG
    CAGCAGTTCA ACCGTAATGT GGAGGATATA GAGCTGTGGC TGTATGAGGT TGAGGGGCAT
    TTGGCTTCAG ATGACTATGG AAAAGACCTG ACCAATGTTC AAAACTTGCA GAAAAAACAC
    GCCTTGCTGG AGGCTGATGT TGCAGCTCAC CAGGACCGTA TCGATGGCAT CACTATCCAA
    GCCCGTCAGT TCCAAGAAGC TGGTCATTTT GATGCTGACA ATATCAGGAA GAAACAGGAG
    GCCCTTGTTA ATAGATATGA AGCTTTGAAA GAACCCATGG TTGCACGCAA GCAGAAGCTG
    TCAGACTCTC TGCGTCTTCA ACAGCTTTTC AGGGATGTTG AAGATGAGGA AACCTGGATA
    AGGGAGAAGG AACCCATTGC AGCGTCCACC AACAGAGGCA AGGATTTGAT TGGAGTCCAG
    AATCTCCTGA AGAAACACCA GGCTTTGCAG GCTGAAATTG CAGGGCACGA ACCACGAATT
    AAAGCTGTTA CCCAGAAAGG AGAATCTATG ATCTCTGAAG GCCACTTTGC TTCTGATGAA
    GTGAAGGGCA AATTAAGGGA GCTGAATGAT AAGTGGGCCA CCCTGAAGAA CAAAGCATCT
    CAGCGCCGAC AGGACCTTGA AGATTCTCTG CAAGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCAGAGT CCTGGATGAG AGAGAAGGAA CCTATTGTTG GAAGCACCGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC CCTTCTGAAG AAACATGAAG CGCTTATGTC AGATCTGCGA
    GCATACGGAA GCAGTATTCA GGGACTTAGA GAGCAGGCCC AGGCCTGCAG GCAACAAGTT
    GCTCCAACTG ATGATGAAAC TGGAAAGGAG CTGGTGCTGG CATTGTATGA TTATCAAGAG
    AAGAGCCCTA GGGAGGTGAC AATGAAGAAA GGAGACATCT TGACCTTGCT AAACAGCACA
    AATAAGGATT GGTGGAAGGT GGAAGTGAAT GATCGTCAGG GCTTTGTACC TGCTGCGTAT
    GTGAAAAAGC TGGACCCAGC ACAGTCCGCA TCCCGGGAAA ACTTGCTGGA GGAACAGGGA
    AGCATCGCTC TCCGTCAGGA GCAGATCGAT GGCCAGGCTC ACATGTCTAA GGAAGCTGGC
    AGTGTTTCTC TGCGTATGAA TCAGGTTGAA GAATTGTATC ATAATATTTT GGAGATGGGA
    GAGAAGCGCA AAGATATGTT AGAAAAGAGC TGCAAGAAGT TTATGCTGTT CCGTGAGGCA
    AATGAGCTGC AGCAGTGGAT TAACGAGAAA GAAGCATCAC TCACTAACGA AGAGGTCGGG
    GCTGACCTGG AACAAGTGGA AGTGCTGCAG AAGAAGTTTG ATGATTTCCA GAAGGATCTG
    AAAGCAAATG AGTCACGCTT GAAGGACATC AACAAGGTGG CCGACGAGCT TGAATCTGAA
    GGCCTCATTA CAGAGGAAGT CCAGCCGGTG CAGCATCAGG AGGTGTATGG GATGCAGCGG
    GATGAAGCAG ACTCCAAATC GGCATCTCCA TGGAAGTCTG TTCGCACAGC TGTGCTCGCG
    GTGGCCACCT TCAACTCTAT CAAGGACTTG AACGAGCGCT GGAAATCACT TCAACAGCTG
    GCAGAAGAAA GGAGTCAGCT TCTTGGCAGT GCCCATGAAG TACAGAGATT CCACAGAGAT
    GCTGATGAGA CAAAAGAATG GATCGAAGAG AAGAACCAGG CTTTGAATAC AGACAGCTAT
    GGACATGACT TGGCCAGTGT GCAGGCTCTG CAACGAAAAC ATGAAGGTTT TGAGAGAGAC
    CTCGCCGCTC TGGGAGATAA GGTGAACTCT TTGGGAGAAA CTGCTGAGCG CCTGATCCAG
    TCACACCCTG AGTCTGCAGA TGACATTCAG GAAAAATGCA CAGAGCTGAA TCAGGCCTGG
    AACAGCCTTG GCACCCGGGC AAACCAGCGC AAGGAGAAAC TAGGCGATTC TCATGATCTG
    CAACGCTTCC TAAGTGACTT CAGAGATCTC ATGTCCTGGA TCAATGGAAT TCGGGGCTTG
    GTGTCATCAG ATGAACTTGC TAGAGATGTG ACTGGTGCAG AGGCTTTGCT GGAACGACAC
    CAGGAGCACA GGACTGAGAT TGATGCCAGA GCAGGCACTT TTCAGGCCTT TGAGCAGTTT
    GGGCAGCAAC TTCTGGCTCG TGGTCATTAT GATAGTCCTG AAATCAAGGA GAAGCTGGAT
    ATCTTGGAAC AGGAACGGGC AAGTTTGGAA AAGGCTTGGG TGGAACGCAG AATGATGTTG
    GATCAGTGCT TGGAGCTGCA GCTCTTCAAC AGAGACTGCG AGCAGGCAGA GAACTGGATG
    GCAGCCCGTG AAGCCTTCCT GAACAGTGAG GATAAAGGGG ATTCCCTGGA CAGTGTGGAG
    GCCCTTATCA AGAAACATGA AGACTTTGAT AAAGCCATTA ATGTCCAGGA AGAGAAGATT
    GCTGCCCTCC AGGCCTTCGC CGAACAATTG ATAATTGCTG ACCACTATGC CAAAGGAGAT
    ATTGCCTCCC GTCGCAATGA AGTCCTGGAC AGGTGGCGCC GTCTCAAGGC TCAGATGATT
    GAGAAGAGAT CCAAACTTGG GGAATCTCAG ACTCTGCAGC AGTTCAGCAG GGACGTCGAT
    GAGATCGAAG CCTGGATCAG CGAGAAATTG CAGACCGCCA GTGACGAGTC CTACAAAGAC
    CCCACAAACA TCCAGAGCAA ACACCAGAAG CACCAGGCTT TCGAAGCTGA GCTCCATGCC
    AACGCTGACC GTATTCGTGG AGTAGTAGAT ATGGGGAACT CCCTTATCGA ACGAGGTGCC
    TGTGCTGGCA GCGAGGATGC GGTGAAGGCT CGCCTAAGCG CACTCGCTGA TCAATGGCAG
    TTCCTTGTCA GCAAATCCGC AGAGAAGAGC CAGAAACTGA AGGAGGCAAA CAAGCAGCAG
    AACTTTAACA CTGGCATCAA AGACTTTGAT TTCTGGTTAA CAGAGGTGGA GGCTTTGCTG
    GCATCTGAAG ACTATGGGAA GGACTTGGCA TCTGTTAACA ACCTGCTGAA GAAACACCAG
    CTTCTGGAAG CTGACATCTC TGCTCATGAG GACCGCCTTA AAGATCTTAA TGGGCAGGCT
    GATAGCCTGA TGACCAGCAG CGCCTTTGAC ACCACCCAAG TAAAGGAGAA AAGGGACGCC
    ATCAACGACC GCTTCCAGCG TATCAAAAGC ATGGCCACCG CACGCCGCAG CAGGCTGAAT
    GAGTCCCACC GTCTGCACCA GTTCTTCAGA GATCTTGATG ATGAGGAATC ATGGATCAAG
    GAAAAGAAAC TTTTGGTCGG ATCTGATGAC TATGGACGTG ACTTGACTGG AGTGCAAAAT
    CTGAGGAAGA AACACAAGAG ATTAGAAGCT GAACTGGCAG CTCATGAGCC AGCCATTCAG
    GGTGTGCTGG ACACTGGGAA GAAGCTTGCA GATGACAACA CTATTGGGAA AGATGAGATT
    CAGCAGCGAC TGGCACAGTT TCTAGAGCAC TGGAAGGAGC TGAAGGAACA AGCAGCGGCT
    CGTGGTCAGA GACTGGAGGA ATCTCTTGAG TACCAACAAT TTGTAGCCAA TGTGGAGGAA
    GAGGAAGCCT GGATTAATGA GAAGATGACT CTAGTGGCCA GTGAAGACTA TGGTGACACA
    CTGGCTGCCA TTCAGGGATT GCTGAAAAAG CACGAGGCAT TTGAGACAGA CTTTACAGTG
    CACAAAGACA GAGTACATGA TGTATGTGCC AACGGGGAAG ACCTGATACA AAAGAACAAT
    CACCATGTGG AGAATATTAG CGCCAAAATG AAGGGTCTCC GAGGGAAGGT GGCAGAACTA
    GAGAAGGCGG CTGCACAGCG CAAGGCCAAA CTTGATGAGA ATTCAGCTTT CCTGCAGTTC
    AACTGGAAGG CGGATGTTGT GGAGTCCTGG ATCGGTGAAA AAGAGAACAG CCTGAAGACT
    GATGATTATG GCAGAGACCT GTCCTCTGTG CAGACCCTTC TCACTAAGCA GGAAACATTT
    GATGCTGGGC TGCAAGCTTT CCAGCAAGAG GGCATTACCA ACATCACTGC ACTGAAAGAC
    CAGCTTCTGG CTGCAAAGCA TGTGCAGTCC CGGGCCATAG AGTCTCGTCA CGCTTCTCTG
    ATGAAGCGGT GGAACCAGTT GTTGGATAAC TCTGCTGCCC GCAAAAAGAA GTTATTGGAG
    GCCCAGGAAC ACTACAGAAA GGTTGAGGAC TTGTTCCTAA CATTTGCCAA GAAAGCCTCT
    GCCTTCAACA GCTGGTTTGA GAATGCCGAG GAGGATTTGA CTGATCCTGT TCGCTGCAAC
    TCCCTGGAGG AAATCAAGGC GCTTCGCGAG GCACATGACG CCTTCCGCAA TTCCCTCAGC
    TCCGCACAGA CAGATTTCAA CCAGCTGGCC GAACTGGACA GGCAAATAAA GGGCTACCGG
    GTAGCCTCCA ATCCCTACAC GTGGTTCACG ATGGAGGCTT TGGAGGAGAC ATGGCGCAAC
    CTGCAGAAAA TCATTAAGGA ACGAGAACTT GAGCTACAAA AAGAACAACG TAGGCAGGAA
    GAGAACGATA AGCTCCGGCA AGAGTTTGCT CAGCACGCCA ATGCTTTCCA CCAGTGGATT
    CAGGAGACAA GGACCTACCT TCTGGACGGG TCCTGTATGG TGGAAGAATC TGGAACACTG
    GAGTCACAAC TTGAAGCTAC AAAGAGAAAG CATCAGGAGA TCCGGGCCAT GAGGAGTCAA
    CTGAAGAAGA TAGAAGATCT TGGCGCAGCC ATGGAAGAGG CCCTGATCTT GGATAATAAA
    TACACAGAAC ACAGCACTGT TGGGCTGGCA CAGCAATGGG ACCAACTGGA TCAGCTAGGC
    ATGAGAATGC AACACAACCT AGAGCAACAG ATCCAGGCCA GAAACACAAC TGGGGTCACA
    GAGGAAGCAC TCAAAGAATT CAGCATGATG TTCAAGCATT TCGACAAAGA CAAGTCTGGC
    CGCCTCAACC ACCAAGAATT CAAATCTTGT CTGCGGTCCC TTGGCTATGA TCTGCCCATG
    GTAGAAGAAG GAGAACCAGA CCCAGAGTTT GAAGCCATTT TGGACACTGT AGATCCCAAC
    AGAGATGGGC ACGTTTCTCT ACAGGAGTAC ATGGCTTTCA TGATTAGCAG AGAAACTGAA
    AACGTCAAGT CCAGTGAGGA GATAGAGAGC GCCTTCCGAG CACTGAGTAC AGACCAGAAA
    CCTTATGTCA CAAAGGAAGA ACTATACCAG AACCTGACAC GCGAACAGGC AGATTACTGC
    ATCTCGCATA TGAAACCCTA CATGGACAGC AAGGGCCGCG AACTGCCCTC TGCATACGAC
    TACGTGGAAT TCACGCGCTC GCTATTTGTG AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29834
    Clone ID Related Accession (Same CDS sequence) XM_012967792.2 , XM_031906805.1 , XM_012967793.1
    Accession Version XM_012967792.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7431bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012967792.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGATTCAG GTGGCGTGAA AGTGCTGGAG ACAGCGGAGG ACATCCAGGA AAGGCGCCAG 
    CAAGTCCTAG ACCGATACCT GCGGTTTAAG GAACTCTCTG TACTGAGACG TCAGAAGTTG
    GAGGATTCTT ACCGCTTCCA GTTCTTCCAG CGAGATGCTG ATGAACTGGA GAAATGGATC
    CAGGAGAAAC TCCAGATCGC ATCTGATGAG AACTACAAAG ATCCAACCAA TCTGCAGGGC
    AAATTGCAGA AACACCAGGC TTTTGAGGCA GAAGTACAGG CCAATGCAGG GGCAATTGTC
    AAACTGGACG AAACTGGAAA CCTGATGATT TCAGAAGGAC ATTTTTCATC AGAGACCATC
    AGAACCCGCC TGGTAGAGTT GCATCGTCTG TGGGAGCTGC TGCTCTTAAA GATGCAAGAG
    AAAGGGGTTA AATTACAACA GGCGCAGAAG CTTGTTCAGT ATCTACGGGA ATGTGAAGAT
    GTCCTAGACT GGATTAATGA CAAGGAGGCT ATTGTAACAT CTGAGGAGCT TGGTCAGGAT
    TTGGAACATG TGGAGGTCTT ACAGAAGAAG TTTGAAGAGT TTCAGACTGA TCTGGCAGCT
    CATGAAGAAA GAGTGAATGA AGTCAACCAG TTTGCAAGTA AACTGGCCCA GGAGGAACAC
    CCGGAGCTGG AGCTAATAAA GACCAAGCAG GATGAAGTCA ATGCAAGTTG GCAGCGCCTC
    AAAGGGCTGG CACTTCAGAG ACAAGGGAAG TTATTTGGAG CTGCAGAAGT TCAGCGTTTT
    AACAGAGATG TTGATGAAAC CATTGGATGG ATAAGAGAGA AGGAGCAGCT GATGGCATCT
    GATGACTTTG GCCGAGACCT GGCTAGCGTT CAAGCTCTCC TACGTAAACA CGAGGGTCTG
    GAGAGAGACC TTGCAGCACT GGAGGAAAAG GTAAAGGCCT TGTGTGCAGA ATCAGACCGT
    CTGCAGCAAT CCCATCCCAT GAACGCCCCT CAAATTCAAG TGAAAGGAGA AGAACTGATA
    ACCAACTGGG AGCAAATACG CACATTGGCA GCAGAACGAC ATGCTCGACT CAATGACTCT
    TACAAGCTTC AGCGTTTCCT TGCAGATTTT AGAGATCTGA CTAGCTGGGT GACTGAAATG
    AAAGCTCTGA TTAATGCCGA TGAGCTTGCC AACGATGTGG CTGGCGCAGA GGCTCTGCTT
    GACCGACACC AGGAACATAA GGGGGAAATT GATGCTCATG AAGATAGCTT TAAAGCTGCA
    GATGCTGCAG GTCAGGCCCT TCTGAACGGT GGCCACTACG CCTCAGATGA AGTCCGAGAA
    AAGCTTACTA TCTTAGCAGA AGAAAGGGCA GCTCTTCTAG AGCTTTGGGA GCTCCGTCGT
    CAGCAGTATG AACAGTGCAT GGACCTGCAG CTTTTTTACA GAGACACCGA ACAGGTGGAC
    AACTGGATGA GCAAACAGGA AGCTTTCCTG CTTAACGAGG ACTTGGGTGA TTCTCTAGAC
    AGTGTGGAAG CTCTTCTGAA AAAACATGAG GACTTTGAGA AGTCTCTTAG CGCCCAGGAA
    GAGAAAATCA CAGCTCTGGA TGAGTTTGCT ACAAAACTAA TTCAAAATAA CCACTATGCC
    AAGGAAGATG TGGCTACCCG CAGAGATGCT CTGCTGAGCC GCCGCAATGC ACTTCATGAA
    CGTGCACTCT ACCGCCGTAC TCAACTGGCA GACTCCTTTC ACCTGCAGCA GTTCTTTAGG
    GACTCTGACG AGCTGAAAAG TTGGATTAAT GAAAAGATGA AGACTGCAAC GGATGAGGCA
    TACAAGGATC CCTCCAACCT CCAGGGTAAG GTGCAGAAAC ACCAAGCTTT TGAGGCTGAA
    CTCTCCGCTA ACCAAAGCCG TATAGATGCC CTGGAGAATT CTGGGCAGAA ATTGATTGAT
    GTCAACCATT ATGCATCAGA TGAGGTGGCA GCACGTATGA GTGAGGTCAT CACTTTGTGG
    AAGAAGCTGC TGGAGGCCAC TGAATTGAAG GGGATCAAGC TGCGCGAAGC CAACCAGCAG
    CAGCAGTTCA ACCGTAATGT GGAGGATATA GAGCTGTGGC TGTATGAGGT TGAGGGGCAT
    TTGGCTTCAG ATGACTATGG AAAAGACCTG ACCAATGTTC AAAACTTGCA GAAAAAACAC
    GCCTTGCTGG AGGCTGATGT TGCAGCTCAC CAGGACCGTA TCGATGGCAT CACTATCCAA
    GCCCGTCAGT TCCAAGAAGC TGGTCATTTT GATGCTGACA ATATCAGGAA GAAACAGGAG
    GCCCTTGTTA ATAGATATGA AGCTTTGAAA GAACCCATGG TTGCACGCAA GCAGAAGCTG
    TCAGACTCTC TGCGTCTTCA ACAGCTTTTC AGGGATGTTG AAGATGAGGA AACCTGGATA
    AGGGAGAAGG AACCCATTGC AGCGTCCACC AACAGAGGCA AGGATTTGAT TGGAGTCCAG
    AATCTCCTGA AGAAACACCA GGCTTTGCAG GCTGAAATTG CAGGGCACGA ACCACGAATT
    AAAGCTGTTA CCCAGAAAGG AGAATCTATG ATCTCTGAAG GCCACTTTGC TTCTGATGAA
    GTGAAGGGCA AATTAAGGGA GCTGAATGAT AAGTGGGCCA CCCTGAAGAA CAAAGCATCT
    CAGCGCCGAC AGGACCTTGA AGATTCTCTG CAAGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCAGAGT CCTGGATGAG AGAGAAGGAA CCTATTGTTG GAAGCACCGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC CCTTCTGAAG AAACATGAAG CGCTTATGTC AGATCTGCGA
    GCATACGGAA GCAGTATTCA GGGACTTAGA GAGCAGGCCC AGGCCTGCAG GCAACAAGTT
    GCTCCAACTG ATGATGAAAC TGGAAAGGAG CTGGTGCTGG CATTGTATGA TTATCAAGAG
    AAGAGCCCTA GGGAGGTGAC AATGAAGAAA GGAGACATCT TGACCTTGCT AAACAGCACA
    AATAAGGATT GGTGGAAGGT GGAAGTGAAT GATCGTCAGG GCTTTGTACC TGCTGCGTAT
    GTGAAAAAGC TGGACCCAGC ACAGTCCGCA TCCCGGGAAA ACTTGCTGGA GGAACAGGGA
    AGCATCGCTC TCCGTCAGGA GCAGATCGAT GGCCAGGCTC ACATGTCTAA GGAAGCTGGC
    AGTGTTTCTC TGCGTATGAA TCAGGTTGAA GAATTGTATC ATAATATTTT GGAGATGGGA
    GAGAAGCGCA AAGATATGTT AGAAAAGAGC TGCAAGAAGT TTATGCTGTT CCGTGAGGCA
    AATGAGCTGC AGCAGTGGAT TAACGAGAAA GAAGCATCAC TCACTAACGA AGAGGTCGGG
    GCTGACCTGG AACAAGTGGA AGTGCTGCAG AAGAAGTTTG ATGATTTCCA GAAGGATCTG
    AAAGCAAATG AGTCACGCTT GAAGGACATC AACAAGGTGG CCGACGAGCT TGAATCTGAA
    GGCCTCATTA CAGAGGAAGT CCAGCCGGTG CAGCATCAGG AGGTGTATGG GATGCAGCGG
    GATGAAGCAG ACTCCAAATC GGCATCTCCA TGGAAGTCTG TTCGCACAGC TGTGCTCGCG
    GTGGCCACCT TCAACTCTAT CAAGGACTTG AACGAGCGCT GGAAATCACT TCAACAGCTG
    GCAGAAGAAA GGAGTCAGCT TCTTGGCAGT GCCCATGAAG TACAGAGATT CCACAGAGAT
    GCTGATGAGA CAAAAGAATG GATCGAAGAG AAGAACCAGG CTTTGAATAC AGACAGCTAT
    GGACATGACT TGGCCAGTGT GCAGGCTCTG CAACGAAAAC ATGAAGGCTT TGAGAGAGAC
    CTCGCCGCTC TGGGAGATAA GGTGAACTCT TTGGGAGAAA CTGCTGAGCG CCTGATCCAG
    TCACACCCTG AGTCTGCAGA TGACATTCAG GAAAAATGCA CAGAGCTGAA TCAGGCCTGG
    AACAGCCTTG GCACCCGGGC AAACCAGCGC AAGGAGAAAC TAGGCGATTC TCATGATCTG
    CAACGCTTCC TAAGTGACTT CAGAGATCTC ATGTCCTGGA TCAATGGAAT TCGGGGCTTG
    GTGTCATCAG ATGAACTTGC TAGAGATGTG ACTGGTGCAG AGGCTTTGCT GGAACGACAC
    CAGGAGCACA GGACTGAGAT TGATGCCAGA GCAGGCACTT TTCAGGCCTT TGAGCAGTTT
    GGGCAGCAAC TTCTGGCTCG TGGTCATTAT GATAGTCCTG AAATCAAGGA GAAGCTGGAT
    ATCTTGGAAC AGGAACGGGC AAGTTTGGAA AAGGCTTGGG TGGAACGCAG AATGATGTTG
    GATCAGTGCT TGGAGCTGCA GCTCTTCAAC AGAGACTGCG AGCAGGCAGA GAACTGGATG
    GCAGCCCGTG AAGCCTTCCT GAACAGTGAG GATAAAGGGG ATTCCCTGGA CAGTGTGGAG
    GCCCTTATCA AGAAACATGA AGACTTTGAT AAAGCCATTA ATGTCCAGGA AGAGAAGATT
    GCTGCCCTCC AGGCCTTCGC CGAACAATTG ATAATTGCTG ACCACTATGC CAAAGGAGAT
    ATTGCCTCCC GTCGCAATGA AGTCCTGGAC AGGTGGCGCC GTCTCAAGGC TCAGATGATT
    GAGAAGAGAT CCAAACTTGG GGAATCTCAG ACTCTGCAGC AGTTCAGCAG GGACGTCGAT
    GAGATCGAAG CCTGGATCAG CGAGAAATTG CAGACCGCCA GTGACGAGTC CTACAAAGAC
    CCCACAAACA TCCAGCTTTC CAAATTGCTG AGCAAACACC AGAAGCACCA GGCTTTCGAA
    GCTGAGCTCC ATGCCAACGC TGACCGTATT CGTGGAGTAG TAGATATGGG GAACTCCCTT
    ATCGAACGAG GTGCCTGTGC TGGCAGCGAG GATGCGGTGA AGGCTCGCCT AAGCGCACTC
    GCTGATCAAT GGCAGTTCCT TGTCAGCAAA TCCGCAGAGA AGAGCCAGAA ACTGAAGGAG
    GCAAACAAGC AGCAGAACTT TAACACTGGC ATCAAAGACT TTGATTTCTG GTTAACAGAG
    GTGGAGGCTT TGCTGGCATC TGAAGACTAT GGGAAGGACT TGGCATCTGT TAACAACCTG
    CTGAAGAAAC ACCAGCTTCT GGAAGCTGAC ATCTCTGCTC ATGAGGACCG CCTTAAAGAT
    CTTAATGGGC AGGCTGATAG CCTGATGACC AGCAGCGCCT TTGACACCAC CCAAGTAAAG
    GAGAAAAGGG ACGCCATCAA CGACCGCTTC CAGCGTATCA AAAGCATGGC CACCGCACGC
    CGCAGCAGGC TGAATGAGTC CCACCGTCTG CACCAGTTCT TCAGAGATCT TGATGATGAG
    GAATCATGGA TCAAGGAAAA GAAACTTTTG GTCGGATCTG ATGACTATGG ACGTGACTTG
    ACTGGAGTGC AAAATCTGAG GAAGAAACAC AAGAGATTAG AAGCTGAACT GGCAGCTCAT
    GAGCCAGCCA TTCAGGGTGT GCTGGACACT GGGAAGAAGC TTGCAGATGA CAACACTATT
    GGGAAAGATG AGATTCAGCA GCGACTGGCA CAGTTTCTAG AGCACTGGAA GGAGCTGAAG
    GAACAAGCAG CGGCTCGTGG TCAGAGACTG GAGGAATCTC TTGAGTACCA ACAATTTGTA
    GCCAATGTGG AGGAAGAGGA AGCCTGGATT AATGAGAAGA TGACTCTAGT GGCCAGTGAA
    GACTATGGTG ACACACTGGC TGCCATTCAG GGATTGCTGA AAAAGCACGA GGCATTTGAG
    ACAGACTTTA CAGTGCACAA AGACAGAGTA CATGATGTAT GTGCCAACGG GGAAGACCTG
    ATACAAAAGA ACAATCACCA TGTGGAGAAT ATTAGCGCCA AAATGAAGGG TCTCCGAGGG
    AAGGTGGCAG AACTAGAGAA GGCGGCTGCA CAGCGCAAGG CCAAACTTGA TGAGAATTCA
    GCTTTCCTGC AGTTCAACTG GAAGGCGGAT GTTGTGGAGT CCTGGATCGG TGAAAAAGAG
    AACAGCCTGA AGACTGATGA TTATGGCAGA GACCTGTCCT CTGTGCAGAC CCTTCTCACT
    AAGCAGGAAA CATTTGATGC TGGGCTGCAA GCTTTCCAGC AAGAGGGCAT TACCAACATC
    ACTGCACTGA AAGACCAGCT TCTGGCTGCA AAGCATGTGC AGTCCCGGGC CATAGAGTCT
    CGTCACGCTT CTCTGATGAA GCGGTGGAAC CAGTTGTTGG ATAACTCTGC TGCCCGCAAA
    AAGAAGTTAT TGGAGGCCCA GGAACACTAC AGAAAGGTTG AGGACTTGTT CCTAACATTT
    GCCAAGAAAG CCTCTGCCTT CAACAGCTGG TTTGAGAATG CCGAGGAGGA TTTGACTGAT
    CCTGTTCGCT GCAACTCCCT GGAGGAAATC AAGGCGCTTC GCGAGGCACA TGACGCCTTC
    CGCAATTCCC TCAGCTCCGC ACAGACAGAT TTCAACCAGC TGGCCGAACT GGACAGGCAA
    ATAAAGGGCT ACCGGGTAGC CTCCAATCCC TACACGTGGT TCACGATGGA GGCTTTGGAG
    GAGACATGGC GCAACCTGCA GAAAATCATT AAGGAACGAG AACTTGAGCT ACAAAAAGAA
    CAACGTAGGC AGGAAGAGAA CGATAAGCTC CGGCAAGAGT TTGCTCAGCA CGCCAATGCT
    TTCCACCAGT GGATTCAGGA GACAAGGACC TACCTTCTGG ACGGGTCCTG TATGGTGGAA
    GAATCTGGAA CACTGGAGTC ACAACTTGAA GCTACAAAGA GAAAGCATCA GGAGATCCGG
    GCCATGAGGA GTCAACTGAA GAAGATAGAA GATCTTGGCG CAGCCATGGA AGAGGCCCTG
    ATCTTGGATA ATAAATACAC AGAACACAGC ACTGTTGGGC TGGCACAGCA ATGGGACCAA
    CTGGATCAGC TAGGCATGAG AATGCAACAC AACCTAGAGC AACAGATCCA GGCCAGAAAC
    ACAACTGGGG TCACAGAGGA AGCACTCAAA GAATTCAGCA TGATGTTCAA GCATTTCGAC
    AAAGACAAGT CTGGCCGCCT CAACCACCAA GAATTCAAAT CTTGTCTGCG GTCCCTTGGC
    TATGATCTGC CCATGGTAGA AGAAGGAGAA CCAGACCCAG AGTTTGAAGC CATTTTGGAC
    ACTGTAGATC CCAACAGAGA TGGGCACGTT TCTCTACAGG AGTACATGGC TTTCATGATT
    AGCAGAGAAA CTGAAAACGT CAAGTCCAGT GAGGAGATAG AGAGCGCCTT CCGAGCACTG
    AGTACAGACC AGAAACCTTA TGTCACAAAG GAAGAACTAT ACCAGAACCT GACACGCGAA
    CAGGCAGATT ACTGCATCTC GCATATGAAA CCCTACATGG ACAGCAAGGG CCGCGAACTG
    CCCTCTGCAT ACGACTACGT GGAATTCACG CGCTCGCTAT TTGTGAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012823246.1
    CDS264..7694
    Translation

    Target ORF information:

    RefSeq Version XM_012967792.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis spectrin, alpha, non-erythrocytic 1 (sptan1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012967792.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGATTCAG GTGGCGTGAA AGTGCTGGAG ACAGCGGAGG ACATCCAGGA AAGGCGCCAG 
    CAAGTCCTAG ACCGATACCT GCGGTTTAAG GAACTCTCTG TACTGAGACG TCAGAAGTTG
    GAGGATTCTT ACCGCTTCCA GTTCTTCCAG CGAGATGCTG ATGAACTGGA GAAATGGATC
    CAGGAGAAAC TCCAGATCGC ATCTGATGAG AACTACAAAG ATCCAACCAA TCTGCAGGGC
    AAATTGCAGA AACACCAGGC TTTTGAGGCA GAAGTACAGG CCAATGCAGG GGCAATTGTC
    AAACTGGACG AAACTGGAAA CCTGATGATT TCAGAAGGAC ATTTTTCATC AGAGACCATC
    AGAACCCGCC TGGTAGAGTT GCATCGTCTG TGGGAGCTGC TGCTCTTAAA GATGCAAGAG
    AAAGGGGTTA AATTACAACA GGCGCAGAAG CTTGTTCAGT ATCTACGGGA ATGTGAAGAT
    GTCCTAGACT GGATTAATGA CAAGGAGGCT ATTGTAACAT CTGAGGAGCT TGGTCAGGAT
    TTGGAACATG TGGAGGTCTT ACAGAAGAAG TTTGAAGAGT TTCAGACTGA TCTGGCAGCT
    CATGAAGAAA GAGTGAATGA AGTCAACCAG TTTGCAAGTA AACTGGCCCA GGAGGAACAC
    CCGGAGCTGG AGCTAATAAA GACCAAGCAG GATGAAGTCA ATGCAAGTTG GCAGCGCCTC
    AAAGGGCTGG CACTTCAGAG ACAAGGGAAG TTATTTGGAG CTGCAGAAGT TCAGCGTTTT
    AACAGAGATG TTGATGAAAC CATTGGATGG ATAAGAGAGA AGGAGCAGCT GATGGCATCT
    GATGACTTTG GCCGAGACCT GGCTAGCGTT CAAGCTCTCC TACGTAAACA CGAGGGTCTG
    GAGAGAGACC TTGCAGCACT GGAGGAAAAG GTAAAGGCCT TGTGTGCAGA ATCAGACCGT
    CTGCAGCAAT CCCATCCCAT GAACGCCCCT CAAATTCAAG TGAAAGGAGA AGAACTGATA
    ACCAACTGGG AGCAAATACG CACATTGGCA GCAGAACGAC ATGCTCGACT CAATGACTCT
    TACAAGCTTC AGCGTTTCCT TGCAGATTTT AGAGATCTGA CTAGCTGGGT GACTGAAATG
    AAAGCTCTGA TTAATGCCGA TGAGCTTGCC AACGATGTGG CTGGCGCAGA GGCTCTGCTT
    GACCGACACC AGGAACATAA GGGGGAAATT GATGCTCATG AAGATAGCTT TAAAGCTGCA
    GATGCTGCAG GTCAGGCCCT TCTGAACGGT GGCCACTACG CCTCAGATGA AGTCCGAGAA
    AAGCTTACTA TCTTAGCAGA AGAAAGGGCA GCTCTTCTAG AGCTTTGGGA GCTCCGTCGT
    CAGCAGTATG AACAGTGCAT GGACCTGCAG CTTTTTTACA GAGACACCGA ACAGGTGGAC
    AACTGGATGA GCAAACAGGA AGCTTTCCTG CTTAACGAGG ACTTGGGTGA TTCTCTAGAC
    AGTGTGGAAG CTCTTCTGAA AAAACATGAG GACTTTGAGA AGTCTCTTAG CGCCCAGGAA
    GAGAAAATCA CAGCTCTGGA TGAGTTTGCT ACAAAACTAA TTCAAAATAA CCACTATGCC
    AAGGAAGATG TGGCTACCCG CAGAGATGCT CTGCTGAGCC GCCGCAATGC ACTTCATGAA
    CGTGCACTCT ACCGCCGTAC TCAACTGGCA GACTCCTTTC ACCTGCAGCA GTTCTTTAGG
    GACTCTGACG AGCTGAAAAG TTGGATTAAT GAAAAGATGA AGACTGCAAC GGATGAGGCA
    TACAAGGATC CCTCCAACCT CCAGGGTAAG GTGCAGAAAC ACCAAGCTTT TGAGGCTGAA
    CTCTCCGCTA ACCAAAGCCG TATAGATGCC CTGGAGAATT CTGGGCAGAA ATTGATTGAT
    GTCAACCATT ATGCATCAGA TGAGGTGGCA GCACGTATGA GTGAGGTCAT CACTTTGTGG
    AAGAAGCTGC TGGAGGCCAC TGAATTGAAG GGGATCAAGC TGCGCGAAGC CAACCAGCAG
    CAGCAGTTCA ACCGTAATGT GGAGGATATA GAGCTGTGGC TGTATGAGGT TGAGGGGCAT
    TTGGCTTCAG ATGACTATGG AAAAGACCTG ACCAATGTTC AAAACTTGCA GAAAAAACAC
    GCCTTGCTGG AGGCTGATGT TGCAGCTCAC CAGGACCGTA TCGATGGCAT CACTATCCAA
    GCCCGTCAGT TCCAAGAAGC TGGTCATTTT GATGCTGACA ATATCAGGAA GAAACAGGAG
    GCCCTTGTTA ATAGATATGA AGCTTTGAAA GAACCCATGG TTGCACGCAA GCAGAAGCTG
    TCAGACTCTC TGCGTCTTCA ACAGCTTTTC AGGGATGTTG AAGATGAGGA AACCTGGATA
    AGGGAGAAGG AACCCATTGC AGCGTCCACC AACAGAGGCA AGGATTTGAT TGGAGTCCAG
    AATCTCCTGA AGAAACACCA GGCTTTGCAG GCTGAAATTG CAGGGCACGA ACCACGAATT
    AAAGCTGTTA CCCAGAAAGG AGAATCTATG ATCTCTGAAG GCCACTTTGC TTCTGATGAA
    GTGAAGGGCA AATTAAGGGA GCTGAATGAT AAGTGGGCCA CCCTGAAGAA CAAAGCATCT
    CAGCGCCGAC AGGACCTTGA AGATTCTCTG CAAGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCAGAGT CCTGGATGAG AGAGAAGGAA CCTATTGTTG GAAGCACCGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC CCTTCTGAAG AAACATGAAG CGCTTATGTC AGATCTGCGA
    GCATACGGAA GCAGTATTCA GGGACTTAGA GAGCAGGCCC AGGCCTGCAG GCAACAAGTT
    GCTCCAACTG ATGATGAAAC TGGAAAGGAG CTGGTGCTGG CATTGTATGA TTATCAAGAG
    AAGAGCCCTA GGGAGGTGAC AATGAAGAAA GGAGACATCT TGACCTTGCT AAACAGCACA
    AATAAGGATT GGTGGAAGGT GGAAGTGAAT GATCGTCAGG GCTTTGTACC TGCTGCGTAT
    GTGAAAAAGC TGGACCCAGC ACAGTCCGCA TCCCGGGAAA ACTTGCTGGA GGAACAGGGA
    AGCATCGCTC TCCGTCAGGA GCAGATCGAT GGCCAGGCTC ACATGTCTAA GGAAGCTGGC
    AGTGTTTCTC TGCGTATGAA TCAGGTTGAA GAATTGTATC ATAATATTTT GGAGATGGGA
    GAGAAGCGCA AAGATATGTT AGAAAAGAGC TGCAAGAAGT TTATGCTGTT CCGTGAGGCA
    AATGAGCTGC AGCAGTGGAT TAACGAGAAA GAAGCATCAC TCACTAACGA AGAGGTCGGG
    GCTGACCTGG AACAAGTGGA AGTGCTGCAG AAGAAGTTTG ATGATTTCCA GAAGGATCTG
    AAAGCAAATG AGTCACGCTT GAAGGACATC AACAAGGTGG CCGACGAGCT TGAATCTGAA
    GGCCTCATTA CAGAGGAAGT CCAGCCGGTG CAGCATCAGG AGGTGTATGG GATGCAGCGG
    GATGAAGCAG ACTCCAAATC GGCATCTCCA TGGAAGTCTG TTCGCACAGC TGTGCTCGCG
    GTGGCCACCT TCAACTCTAT CAAGGACTTG AACGAGCGCT GGAAATCACT TCAACAGCTG
    GCAGAAGAAA GGAGTCAGCT TCTTGGCAGT GCCCATGAAG TACAGAGATT CCACAGAGAT
    GCTGATGAGA CAAAAGAATG GATCGAAGAG AAGAACCAGG CTTTGAATAC AGACAGCTAT
    GGACATGACT TGGCCAGTGT GCAGGCTCTG CAACGAAAAC ATGAAGGCTT TGAGAGAGAC
    CTCGCCGCTC TGGGAGATAA GGTGAACTCT TTGGGAGAAA CTGCTGAGCG CCTGATCCAG
    TCACACCCTG AGTCTGCAGA TGACATTCAG GAAAAATGCA CAGAGCTGAA TCAGGCCTGG
    AACAGCCTTG GCACCCGGGC AAACCAGCGC AAGGAGAAAC TAGGCGATTC TCATGATCTG
    CAACGCTTCC TAAGTGACTT CAGAGATCTC ATGTCCTGGA TCAATGGAAT TCGGGGCTTG
    GTGTCATCAG ATGAACTTGC TAGAGATGTG ACTGGTGCAG AGGCTTTGCT GGAACGACAC
    CAGGAGCACA GGACTGAGAT TGATGCCAGA GCAGGCACTT TTCAGGCCTT TGAGCAGTTT
    GGGCAGCAAC TTCTGGCTCG TGGTCATTAT GATAGTCCTG AAATCAAGGA GAAGCTGGAT
    ATCTTGGAAC AGGAACGGGC AAGTTTGGAA AAGGCTTGGG TGGAACGCAG AATGATGTTG
    GATCAGTGCT TGGAGCTGCA GCTCTTCAAC AGAGACTGCG AGCAGGCAGA GAACTGGATG
    GCAGCCCGTG AAGCCTTCCT GAACAGTGAG GATAAAGGGG ATTCCCTGGA CAGTGTGGAG
    GCCCTTATCA AGAAACATGA AGACTTTGAT AAAGCCATTA ATGTCCAGGA AGAGAAGATT
    GCTGCCCTCC AGGCCTTCGC CGAACAATTG ATAATTGCTG ACCACTATGC CAAAGGAGAT
    ATTGCCTCCC GTCGCAATGA AGTCCTGGAC AGGTGGCGCC GTCTCAAGGC TCAGATGATT
    GAGAAGAGAT CCAAACTTGG GGAATCTCAG ACTCTGCAGC AGTTCAGCAG GGACGTCGAT
    GAGATCGAAG CCTGGATCAG CGAGAAATTG CAGACCGCCA GTGACGAGTC CTACAAAGAC
    CCCACAAACA TCCAGCTTTC CAAATTGCTG AGCAAACACC AGAAGCACCA GGCTTTCGAA
    GCTGAGCTCC ATGCCAACGC TGACCGTATT CGTGGAGTAG TAGATATGGG GAACTCCCTT
    ATCGAACGAG GTGCCTGTGC TGGCAGCGAG GATGCGGTGA AGGCTCGCCT AAGCGCACTC
    GCTGATCAAT GGCAGTTCCT TGTCAGCAAA TCCGCAGAGA AGAGCCAGAA ACTGAAGGAG
    GCAAACAAGC AGCAGAACTT TAACACTGGC ATCAAAGACT TTGATTTCTG GTTAACAGAG
    GTGGAGGCTT TGCTGGCATC TGAAGACTAT GGGAAGGACT TGGCATCTGT TAACAACCTG
    CTGAAGAAAC ACCAGCTTCT GGAAGCTGAC ATCTCTGCTC ATGAGGACCG CCTTAAAGAT
    CTTAATGGGC AGGCTGATAG CCTGATGACC AGCAGCGCCT TTGACACCAC CCAAGTAAAG
    GAGAAAAGGG ACGCCATCAA CGACCGCTTC CAGCGTATCA AAAGCATGGC CACCGCACGC
    CGCAGCAGGC TGAATGAGTC CCACCGTCTG CACCAGTTCT TCAGAGATCT TGATGATGAG
    GAATCATGGA TCAAGGAAAA GAAACTTTTG GTCGGATCTG ATGACTATGG ACGTGACTTG
    ACTGGAGTGC AAAATCTGAG GAAGAAACAC AAGAGATTAG AAGCTGAACT GGCAGCTCAT
    GAGCCAGCCA TTCAGGGTGT GCTGGACACT GGGAAGAAGC TTGCAGATGA CAACACTATT
    GGGAAAGATG AGATTCAGCA GCGACTGGCA CAGTTTCTAG AGCACTGGAA GGAGCTGAAG
    GAACAAGCAG CGGCTCGTGG TCAGAGACTG GAGGAATCTC TTGAGTACCA ACAATTTGTA
    GCCAATGTGG AGGAAGAGGA AGCCTGGATT AATGAGAAGA TGACTCTAGT GGCCAGTGAA
    GACTATGGTG ACACACTGGC TGCCATTCAG GGATTGCTGA AAAAGCACGA GGCATTTGAG
    ACAGACTTTA CAGTGCACAA AGACAGAGTA CATGATGTAT GTGCCAACGG GGAAGACCTG
    ATACAAAAGA ACAATCACCA TGTGGAGAAT ATTAGCGCCA AAATGAAGGG TCTCCGAGGG
    AAGGTGGCAG AACTAGAGAA GGCGGCTGCA CAGCGCAAGG CCAAACTTGA TGAGAATTCA
    GCTTTCCTGC AGTTCAACTG GAAGGCGGAT GTTGTGGAGT CCTGGATCGG TGAAAAAGAG
    AACAGCCTGA AGACTGATGA TTATGGCAGA GACCTGTCCT CTGTGCAGAC CCTTCTCACT
    AAGCAGGAAA CATTTGATGC TGGGCTGCAA GCTTTCCAGC AAGAGGGCAT TACCAACATC
    ACTGCACTGA AAGACCAGCT TCTGGCTGCA AAGCATGTGC AGTCCCGGGC CATAGAGTCT
    CGTCACGCTT CTCTGATGAA GCGGTGGAAC CAGTTGTTGG ATAACTCTGC TGCCCGCAAA
    AAGAAGTTAT TGGAGGCCCA GGAACACTAC AGAAAGGTTG AGGACTTGTT CCTAACATTT
    GCCAAGAAAG CCTCTGCCTT CAACAGCTGG TTTGAGAATG CCGAGGAGGA TTTGACTGAT
    CCTGTTCGCT GCAACTCCCT GGAGGAAATC AAGGCGCTTC GCGAGGCACA TGACGCCTTC
    CGCAATTCCC TCAGCTCCGC ACAGACAGAT TTCAACCAGC TGGCCGAACT GGACAGGCAA
    ATAAAGGGCT ACCGGGTAGC CTCCAATCCC TACACGTGGT TCACGATGGA GGCTTTGGAG
    GAGACATGGC GCAACCTGCA GAAAATCATT AAGGAACGAG AACTTGAGCT ACAAAAAGAA
    CAACGTAGGC AGGAAGAGAA CGATAAGCTC CGGCAAGAGT TTGCTCAGCA CGCCAATGCT
    TTCCACCAGT GGATTCAGGA GACAAGGACC TACCTTCTGG ACGGGTCCTG TATGGTGGAA
    GAATCTGGAA CACTGGAGTC ACAACTTGAA GCTACAAAGA GAAAGCATCA GGAGATCCGG
    GCCATGAGGA GTCAACTGAA GAAGATAGAA GATCTTGGCG CAGCCATGGA AGAGGCCCTG
    ATCTTGGATA ATAAATACAC AGAACACAGC ACTGTTGGGC TGGCACAGCA ATGGGACCAA
    CTGGATCAGC TAGGCATGAG AATGCAACAC AACCTAGAGC AACAGATCCA GGCCAGAAAC
    ACAACTGGGG TCACAGAGGA AGCACTCAAA GAATTCAGCA TGATGTTCAA GCATTTCGAC
    AAAGACAAGT CTGGCCGCCT CAACCACCAA GAATTCAAAT CTTGTCTGCG GTCCCTTGGC
    TATGATCTGC CCATGGTAGA AGAAGGAGAA CCAGACCCAG AGTTTGAAGC CATTTTGGAC
    ACTGTAGATC CCAACAGAGA TGGGCACGTT TCTCTACAGG AGTACATGGC TTTCATGATT
    AGCAGAGAAA CTGAAAACGT CAAGTCCAGT GAGGAGATAG AGAGCGCCTT CCGAGCACTG
    AGTACAGACC AGAAACCTTA TGTCACAAAG GAAGAACTAT ACCAGAACCT GACACGCGAA
    CAGGCAGATT ACTGCATCTC GCATATGAAA CCCTACATGG ACAGCAAGGG CCGCGAACTG
    CCCTCTGCAT ACGACTACGT GGAATTCACG CGCTCGCTAT TTGTGAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29834
    Clone ID Related Accession (Same CDS sequence) XM_012967792.2 , XM_031906805.1 , XM_012967793.1
    Accession Version XM_012967793.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7431bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGATTCAG GTGGCGTGAA AGTGCTGGAG ACAGCGGAGG ACATCCAGGA AAGGCGCCAG 
    CAAGTCCTAG ACCGATACCT GCGGTTTAAG GAACTCTCTG TACTGAGACG TCAGAAGTTG
    GAGGATTCTT ACCGCTTCCA GTTCTTCCAG CGAGATGCTG ATGAACTGGA GAAATGGATC
    CAGGAGAAAC TCCAGATCGC ATCTGATGAG AACTACAAAG ATCCAACCAA TCTGCAGGGC
    AAATTGCAGA AACACCAGGC TTTTGAGGCA GAAGTACAGG CCAATGCAGG GGCAATTGTC
    AAACTGGACG AAACTGGAAA CCTGATGATT TCAGAAGGAC ATTTTTCATC AGAGACCATC
    AGAACCCGCC TGGTAGAGTT GCATCGTCTG TGGGAGCTGC TGCTCTTAAA GATGCAAGAG
    AAAGGGGTTA AATTACAACA GGCGCAGAAG CTTGTTCAGT ATCTACGGGA ATGTGAAGAT
    GTCCTAGACT GGATTAATGA CAAGGAGGCT ATTGTAACAT CTGAGGAGCT TGGTCAGGAT
    TTGGAACATG TGGAGGTCTT ACAGAAGAAG TTTGAAGAGT TTCAGACTGA TCTGGCAGCT
    CATGAAGAAA GAGTGAATGA AGTCAACCAG TTTGCAAGTA AACTGGCCCA GGAGGAACAC
    CCGGAGCTGG AGCTAATAAA GACCAAGCAG GATGAAGTCA ATGCAAGTTG GCAGCGCCTC
    AAAGGGCTGG CACTTCAGAG ACAAGGGAAG TTATTTGGAG CTGCAGAAGT TCAGCGTTTT
    AACAGAGATG TTGATGAAAC CATTGGATGG ATAAGAGAGA AGGAGCAGCT GATGGCATCT
    GATGACTTTG GCCGAGACCT GGCTAGCGTT CAAGCTCTCC TACGTAAACA CGAGGGTCTG
    GAGAGAGACC TTGCAGCACT GGAGGAAAAG GTAAAGGCCT TGTGTGCAGA ATCAGACCGT
    CTGCAGCAAT CCCATCCCAT GAACGCCCCT CAAATTCAAG TGAAAGGAGA AGAACTGATA
    ACCAACTGGG AGCAAATACG CACATTGGCA GCAGAACGAC ATGCTCGACT CAATGACTCT
    TACAAGCTTC AGCGTTTCCT TGCAGATTTT AGAGATCTGA CTAGCTGGGT GACTGAAATG
    AAAGCTCTGA TTAATGCCGA TGAGCTTGCC AACGATGTGG CTGGCGCAGA GGCTCTGCTT
    GACCGACACC AGGAACATAA GGGGGAAATT GATGCTCATG AAGATAGCTT TAAAGCTGCA
    GATGCTGCAG GTCAGGCCCT TCTGAACGGT GGCCACTACG CCTCAGATGA AGTCCGAGAA
    AAGCTTACTA TCTTAGCAGA AGAAAGGGCA GCTCTTCTAG AGCTTTGGGA GCTCCGTCGT
    CAGCAGTATG AACAGTGCAT GGACCTGCAG CTTTTTTACA GAGACACCGA ACAGGTGGAC
    AACTGGATGA GCAAACAGGA AGCTTTCCTG CTTAACGAGG ACTTGGGTGA TTCTCTAGAC
    AGTGTGGAAG CTCTTCTGAA AAAACATGAG GACTTTGAGA AGTCTCTTAG CGCCCAGGAA
    GAGAAAATCA CAGCTCTGGA TGAGTTTGCT ACAAAACTAA TTCAAAATAA CCACTATGCC
    AAGGAAGATG TGGCTACCCG CAGAGATGCT CTGCTGAGCC GCCGCAATGC ACTTCATGAA
    CGTGCACTCT ACCGCCGTAC TCAACTGGCA GACTCCTTTC ACCTGCAGCA GTTCTTTAGG
    GACTCTGACG AGCTGAAAAG TTGGATTAAT GAAAAGATGA AGACTGCAAC GGATGAGGCA
    TACAAGGATC CCTCCAACCT CCAGGGTAAG GTGCAGAAAC ACCAAGCTTT TGAGGCTGAA
    CTCTCCGCTA ACCAAAGCCG TATAGATGCC CTGGAGAATT CTGGGCAGAA ATTGATTGAT
    GTCAACCATT ATGCATCAGA TGAGGTGGCA GCACGTATGA GTGAGGTCAT CACTTTGTGG
    AAGAAGCTGC TGGAGGCCAC TGAATTGAAG GGGATCAAGC TGCGCGAAGC CAACCAGCAG
    CAGCAGTTCA ACCGTAATGT GGAGGATATA GAGCTGTGGC TGTATGAGGT TGAGGGGCAT
    TTGGCTTCAG ATGACTATGG AAAAGACCTG ACCAATGTTC AAAACTTGCA GAAAAAACAC
    GCCTTGCTGG AGGCTGATGT TGCAGCTCAC CAGGACCGTA TCGATGGCAT CACTATCCAA
    GCCCGTCAGT TCCAAGAAGC TGGTCATTTT GATGCTGACA ATATCAGGAA GAAACAGGAG
    GCCCTTGTTA ATAGATATGA AGCTTTGAAA GAACCCATGG TTGCACGCAA GCAGAAGCTG
    TCAGACTCTC TGCGTCTTCA ACAGCTTTTC AGGGATGTTG AAGATGAGGA AACCTGGATA
    AGGGAGAAGG AACCCATTGC AGCGTCCACC AACAGAGGCA AGGATTTGAT TGGAGTCCAG
    AATCTCCTGA AGAAACACCA GGCTTTGCAG GCTGAAATTG CAGGGCACGA ACCACGAATT
    AAAGCTGTTA CCCAGAAAGG AGAATCTATG ATCTCTGAAG GCCACTTTGC TTCTGATGAA
    GTGAAGGGCA AATTAAGGGA GCTGAATGAT AAGTGGGCCA CCCTGAAGAA CAAAGCATCT
    CAGCGCCGAC AGGACCTTGA AGATTCTCTG CAAGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCAGAGT CCTGGATGAG AGAGAAGGAA CCTATTGTTG GAAGCACCGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC CCTTCTGAAG AAACATGAAG CGCTTATGTC AGATCTGCGA
    GCATACGGAA GCAGTATTCA GGGACTTAGA GAGCAGGCCC AGGCCTGCAG GCAACAAGTT
    GCTCCAACTG ATGATGAAAC TGGAAAGGAG CTGGTGCTGG CATTGTATGA TTATCAAGAG
    AAGAGCCCTA GGGAGGTGAC AATGAAGAAA GGAGACATCT TGACCTTGCT AAACAGCACA
    AATAAGGATT GGTGGAAGGT GGAAGTGAAT GATCGTCAGG GCTTTGTACC TGCTGCGTAT
    GTGAAAAAGC TGGACCCAGC ACAGTCCGCA TCCCGGGAAA ACTTGCTGGA GGAACAGGGA
    AGCATCGCTC TCCGTCAGGA GCAGATCGAT GGCCAGGCTC ACATGTCTAA GGAAGCTGGC
    AGTGTTTCTC TGCGTATGAA TCAGGTTGAA GAATTGTATC ATAATATTTT GGAGATGGGA
    GAGAAGCGCA AAGATATGTT AGAAAAGAGC TGCAAGAAGT TTATGCTGTT CCGTGAGGCA
    AATGAGCTGC AGCAGTGGAT TAACGAGAAA GAAGCATCAC TCACTAACGA AGAGGTCGGG
    GCTGACCTGG AACAAGTGGA AGTGCTGCAG AAGAAGTTTG ATGATTTCCA GAAGGATCTG
    AAAGCAAATG AGTCACGCTT GAAGGACATC AACAAGGTGG CCGACGAGCT TGAATCTGAA
    GGCCTCATTA CAGAGGAAGT CCAGCCGGTG CAGCATCAGG AGGTGTATGG GATGCAGCGG
    GATGAAGCAG ACTCCAAATC GGCATCTCCA TGGAAGTCTG TTCGCACAGC TGTGCTCGCG
    GTGGCCACCT TCAACTCTAT CAAGGACTTG AACGAGCGCT GGAAATCACT TCAACAGCTG
    GCAGAAGAAA GGAGTCAGCT TCTTGGCAGT GCCCATGAAG TACAGAGATT CCACAGAGAT
    GCTGATGAGA CAAAAGAATG GATCGAAGAG AAGAACCAGG CTTTGAATAC AGACAGCTAT
    GGACATGACT TGGCCAGTGT GCAGGCTCTG CAACGAAAAC ATGAAGGCTT TGAGAGAGAC
    CTCGCCGCTC TGGGAGATAA GGTGAACTCT TTGGGAGAAA CTGCTGAGCG CCTGATCCAG
    TCACACCCTG AGTCTGCAGA TGACATTCAG GAAAAATGCA CAGAGCTGAA TCAGGCCTGG
    AACAGCCTTG GCACCCGGGC AAACCAGCGC AAGGAGAAAC TAGGCGATTC TCATGATCTG
    CAACGCTTCC TAAGTGACTT CAGAGATCTC ATGTCCTGGA TCAATGGAAT TCGGGGCTTG
    GTGTCATCAG ATGAACTTGC TAGAGATGTG ACTGGTGCAG AGGCTTTGCT GGAACGACAC
    CAGGAGCACA GGACTGAGAT TGATGCCAGA GCAGGCACTT TTCAGGCCTT TGAGCAGTTT
    GGGCAGCAAC TTCTGGCTCG TGGTCATTAT GATAGTCCTG AAATCAAGGA GAAGCTGGAT
    ATCTTGGAAC AGGAACGGGC AAGTTTGGAA AAGGCTTGGG TGGAACGCAG AATGATGTTG
    GATCAGTGCT TGGAGCTGCA GCTCTTCAAC AGAGACTGCG AGCAGGCAGA GAACTGGATG
    GCAGCCCGTG AAGCCTTCCT GAACAGTGAG GATAAAGGGG ATTCCCTGGA CAGTGTGGAG
    GCCCTTATCA AGAAACATGA AGACTTTGAT AAAGCCATTA ATGTCCAGGA AGAGAAGATT
    GCTGCCCTCC AGGCCTTCGC CGAACAATTG ATAATTGCTG ACCACTATGC CAAAGGAGAT
    ATTGCCTCCC GTCGCAATGA AGTCCTGGAC AGGTGGCGCC GTCTCAAGGC TCAGATGATT
    GAGAAGAGAT CCAAACTTGG GGAATCTCAG ACTCTGCAGC AGTTCAGCAG GGACGTCGAT
    GAGATCGAAG CCTGGATCAG CGAGAAATTG CAGACCGCCA GTGACGAGTC CTACAAAGAC
    CCCACAAACA TCCAGCTTTC CAAATTGCTG AGCAAACACC AGAAGCACCA GGCTTTCGAA
    GCTGAGCTCC ATGCCAACGC TGACCGTATT CGTGGAGTAG TAGATATGGG GAACTCCCTT
    ATCGAACGAG GTGCCTGTGC TGGCAGCGAG GATGCGGTGA AGGCTCGCCT AAGCGCACTC
    GCTGATCAAT GGCAGTTCCT TGTCAGCAAA TCCGCAGAGA AGAGCCAGAA ACTGAAGGAG
    GCAAACAAGC AGCAGAACTT TAACACTGGC ATCAAAGACT TTGATTTCTG GTTAACAGAG
    GTGGAGGCTT TGCTGGCATC TGAAGACTAT GGGAAGGACT TGGCATCTGT TAACAACCTG
    CTGAAGAAAC ACCAGCTTCT GGAAGCTGAC ATCTCTGCTC ATGAGGACCG CCTTAAAGAT
    CTTAATGGGC AGGCTGATAG CCTGATGACC AGCAGCGCCT TTGACACCAC CCAAGTAAAG
    GAGAAAAGGG ACGCCATCAA CGACCGCTTC CAGCGTATCA AAAGCATGGC CACCGCACGC
    CGCAGCAGGC TGAATGAGTC CCACCGTCTG CACCAGTTCT TCAGAGATCT TGATGATGAG
    GAATCATGGA TCAAGGAAAA GAAACTTTTG GTCGGATCTG ATGACTATGG ACGTGACTTG
    ACTGGAGTGC AAAATCTGAG GAAGAAACAC AAGAGATTAG AAGCTGAACT GGCAGCTCAT
    GAGCCAGCCA TTCAGGGTGT GCTGGACACT GGGAAGAAGC TTGCAGATGA CAACACTATT
    GGGAAAGATG AGATTCAGCA GCGACTGGCA CAGTTTCTAG AGCACTGGAA GGAGCTGAAG
    GAACAAGCAG CGGCTCGTGG TCAGAGACTG GAGGAATCTC TTGAGTACCA ACAATTTGTA
    GCCAATGTGG AGGAAGAGGA AGCCTGGATT AATGAGAAGA TGACTCTAGT GGCCAGTGAA
    GACTATGGTG ACACACTGGC TGCCATTCAG GGATTGCTGA AAAAGCACGA GGCATTTGAG
    ACAGACTTTA CAGTGCACAA AGACAGAGTA CATGATGTAT GTGCCAACGG GGAAGACCTG
    ATACAAAAGA ACAATCACCA TGTGGAGAAT ATTAGCGCCA AAATGAAGGG TCTCCGAGGG
    AAGGTGGCAG AACTAGAGAA GGCGGCTGCA CAGCGCAAGG CCAAACTTGA TGAGAATTCA
    GCTTTCCTGC AGTTCAACTG GAAGGCGGAT GTTGTGGAGT CCTGGATCGG TGAAAAAGAG
    AACAGCCTGA AGACTGATGA TTATGGCAGA GACCTGTCCT CTGTGCAGAC CCTTCTCACT
    AAGCAGGAAA CATTTGATGC TGGGCTGCAA GCTTTCCAGC AAGAGGGCAT TACCAACATC
    ACTGCACTGA AAGACCAGCT TCTGGCTGCA AAGCATGTGC AGTCCCGGGC CATAGAGTCT
    CGTCACGCTT CTCTGATGAA GCGGTGGAAC CAGTTGTTGG ATAACTCTGC TGCCCGCAAA
    AAGAAGTTAT TGGAGGCCCA GGAACACTAC AGAAAGGTTG AGGACTTGTT CCTAACATTT
    GCCAAGAAAG CCTCTGCCTT CAACAGCTGG TTTGAGAATG CCGAGGAGGA TTTGACTGAT
    CCTGTTCGCT GCAACTCCCT GGAGGAAATC AAGGCGCTTC GCGAGGCACA TGACGCCTTC
    CGCAATTCCC TCAGCTCCGC ACAGACAGAT TTCAACCAGC TGGCCGAACT GGACAGGCAA
    ATAAAGGGCT ACCGGGTAGC CTCCAATCCC TACACGTGGT TCACGATGGA GGCTTTGGAG
    GAGACATGGC GCAACCTGCA GAAAATCATT AAGGAACGAG AACTTGAGCT ACAAAAAGAA
    CAACGTAGGC AGGAAGAGAA CGATAAGCTC CGGCAAGAGT TTGCTCAGCA CGCCAATGCT
    TTCCACCAGT GGATTCAGGA GACAAGGACC TACCTTCTGG ACGGGTCCTG TATGGTGGAA
    GAATCTGGAA CACTGGAGTC ACAACTTGAA GCTACAAAGA GAAAGCATCA GGAGATCCGG
    GCCATGAGGA GTCAACTGAA GAAGATAGAA GATCTTGGCG CAGCCATGGA AGAGGCCCTG
    ATCTTGGATA ATAAATACAC AGAACACAGC ACTGTTGGGC TGGCACAGCA ATGGGACCAA
    CTGGATCAGC TAGGCATGAG AATGCAACAC AACCTAGAGC AACAGATCCA GGCCAGAAAC
    ACAACTGGGG TCACAGAGGA AGCACTCAAA GAATTCAGCA TGATGTTCAA GCATTTCGAC
    AAAGACAAGT CTGGCCGCCT CAACCACCAA GAATTCAAAT CTTGTCTGCG GTCCCTTGGC
    TATGATCTGC CCATGGTAGA AGAAGGAGAA CCAGACCCAG AGTTTGAAGC CATTTTGGAC
    ACTGTAGATC CCAACAGAGA TGGGCACGTT TCTCTACAGG AGTACATGGC TTTCATGATT
    AGCAGAGAAA CTGAAAACGT CAAGTCCAGT GAGGAGATAG AGAGCGCCTT CCGAGCACTG
    AGTACAGACC AGAAACCTTA TGTCACAAAG GAAGAACTAT ACCAGAACCT GACACGCGAA
    CAGGCAGATT ACTGCATCTC GCATATGAAA CCCTACATGG ACAGCAAGGG CCGCGAACTG
    CCCTCTGCAT ACGACTACGT GGAATTCACG CGCTCGCTAT TTGTGAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012823247.1
    CDS102..7532
    Translation

    Target ORF information:

    RefSeq Version XM_012967793.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis spectrin, alpha, non-erythrocytic 1 (sptan1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012967793.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGATTCAG GTGGCGTGAA AGTGCTGGAG ACAGCGGAGG ACATCCAGGA AAGGCGCCAG 
    CAAGTCCTAG ACCGATACCT GCGGTTTAAG GAACTCTCTG TACTGAGACG TCAGAAGTTG
    GAGGATTCTT ACCGCTTCCA GTTCTTCCAG CGAGATGCTG ATGAACTGGA GAAATGGATC
    CAGGAGAAAC TCCAGATCGC ATCTGATGAG AACTACAAAG ATCCAACCAA TCTGCAGGGC
    AAATTGCAGA AACACCAGGC TTTTGAGGCA GAAGTACAGG CCAATGCAGG GGCAATTGTC
    AAACTGGACG AAACTGGAAA CCTGATGATT TCAGAAGGAC ATTTTTCATC AGAGACCATC
    AGAACCCGCC TGGTAGAGTT GCATCGTCTG TGGGAGCTGC TGCTCTTAAA GATGCAAGAG
    AAAGGGGTTA AATTACAACA GGCGCAGAAG CTTGTTCAGT ATCTACGGGA ATGTGAAGAT
    GTCCTAGACT GGATTAATGA CAAGGAGGCT ATTGTAACAT CTGAGGAGCT TGGTCAGGAT
    TTGGAACATG TGGAGGTCTT ACAGAAGAAG TTTGAAGAGT TTCAGACTGA TCTGGCAGCT
    CATGAAGAAA GAGTGAATGA AGTCAACCAG TTTGCAAGTA AACTGGCCCA GGAGGAACAC
    CCGGAGCTGG AGCTAATAAA GACCAAGCAG GATGAAGTCA ATGCAAGTTG GCAGCGCCTC
    AAAGGGCTGG CACTTCAGAG ACAAGGGAAG TTATTTGGAG CTGCAGAAGT TCAGCGTTTT
    AACAGAGATG TTGATGAAAC CATTGGATGG ATAAGAGAGA AGGAGCAGCT GATGGCATCT
    GATGACTTTG GCCGAGACCT GGCTAGCGTT CAAGCTCTCC TACGTAAACA CGAGGGTCTG
    GAGAGAGACC TTGCAGCACT GGAGGAAAAG GTAAAGGCCT TGTGTGCAGA ATCAGACCGT
    CTGCAGCAAT CCCATCCCAT GAACGCCCCT CAAATTCAAG TGAAAGGAGA AGAACTGATA
    ACCAACTGGG AGCAAATACG CACATTGGCA GCAGAACGAC ATGCTCGACT CAATGACTCT
    TACAAGCTTC AGCGTTTCCT TGCAGATTTT AGAGATCTGA CTAGCTGGGT GACTGAAATG
    AAAGCTCTGA TTAATGCCGA TGAGCTTGCC AACGATGTGG CTGGCGCAGA GGCTCTGCTT
    GACCGACACC AGGAACATAA GGGGGAAATT GATGCTCATG AAGATAGCTT TAAAGCTGCA
    GATGCTGCAG GTCAGGCCCT TCTGAACGGT GGCCACTACG CCTCAGATGA AGTCCGAGAA
    AAGCTTACTA TCTTAGCAGA AGAAAGGGCA GCTCTTCTAG AGCTTTGGGA GCTCCGTCGT
    CAGCAGTATG AACAGTGCAT GGACCTGCAG CTTTTTTACA GAGACACCGA ACAGGTGGAC
    AACTGGATGA GCAAACAGGA AGCTTTCCTG CTTAACGAGG ACTTGGGTGA TTCTCTAGAC
    AGTGTGGAAG CTCTTCTGAA AAAACATGAG GACTTTGAGA AGTCTCTTAG CGCCCAGGAA
    GAGAAAATCA CAGCTCTGGA TGAGTTTGCT ACAAAACTAA TTCAAAATAA CCACTATGCC
    AAGGAAGATG TGGCTACCCG CAGAGATGCT CTGCTGAGCC GCCGCAATGC ACTTCATGAA
    CGTGCACTCT ACCGCCGTAC TCAACTGGCA GACTCCTTTC ACCTGCAGCA GTTCTTTAGG
    GACTCTGACG AGCTGAAAAG TTGGATTAAT GAAAAGATGA AGACTGCAAC GGATGAGGCA
    TACAAGGATC CCTCCAACCT CCAGGGTAAG GTGCAGAAAC ACCAAGCTTT TGAGGCTGAA
    CTCTCCGCTA ACCAAAGCCG TATAGATGCC CTGGAGAATT CTGGGCAGAA ATTGATTGAT
    GTCAACCATT ATGCATCAGA TGAGGTGGCA GCACGTATGA GTGAGGTCAT CACTTTGTGG
    AAGAAGCTGC TGGAGGCCAC TGAATTGAAG GGGATCAAGC TGCGCGAAGC CAACCAGCAG
    CAGCAGTTCA ACCGTAATGT GGAGGATATA GAGCTGTGGC TGTATGAGGT TGAGGGGCAT
    TTGGCTTCAG ATGACTATGG AAAAGACCTG ACCAATGTTC AAAACTTGCA GAAAAAACAC
    GCCTTGCTGG AGGCTGATGT TGCAGCTCAC CAGGACCGTA TCGATGGCAT CACTATCCAA
    GCCCGTCAGT TCCAAGAAGC TGGTCATTTT GATGCTGACA ATATCAGGAA GAAACAGGAG
    GCCCTTGTTA ATAGATATGA AGCTTTGAAA GAACCCATGG TTGCACGCAA GCAGAAGCTG
    TCAGACTCTC TGCGTCTTCA ACAGCTTTTC AGGGATGTTG AAGATGAGGA AACCTGGATA
    AGGGAGAAGG AACCCATTGC AGCGTCCACC AACAGAGGCA AGGATTTGAT TGGAGTCCAG
    AATCTCCTGA AGAAACACCA GGCTTTGCAG GCTGAAATTG CAGGGCACGA ACCACGAATT
    AAAGCTGTTA CCCAGAAAGG AGAATCTATG ATCTCTGAAG GCCACTTTGC TTCTGATGAA
    GTGAAGGGCA AATTAAGGGA GCTGAATGAT AAGTGGGCCA CCCTGAAGAA CAAAGCATCT
    CAGCGCCGAC AGGACCTTGA AGATTCTCTG CAAGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCAGAGT CCTGGATGAG AGAGAAGGAA CCTATTGTTG GAAGCACCGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC CCTTCTGAAG AAACATGAAG CGCTTATGTC AGATCTGCGA
    GCATACGGAA GCAGTATTCA GGGACTTAGA GAGCAGGCCC AGGCCTGCAG GCAACAAGTT
    GCTCCAACTG ATGATGAAAC TGGAAAGGAG CTGGTGCTGG CATTGTATGA TTATCAAGAG
    AAGAGCCCTA GGGAGGTGAC AATGAAGAAA GGAGACATCT TGACCTTGCT AAACAGCACA
    AATAAGGATT GGTGGAAGGT GGAAGTGAAT GATCGTCAGG GCTTTGTACC TGCTGCGTAT
    GTGAAAAAGC TGGACCCAGC ACAGTCCGCA TCCCGGGAAA ACTTGCTGGA GGAACAGGGA
    AGCATCGCTC TCCGTCAGGA GCAGATCGAT GGCCAGGCTC ACATGTCTAA GGAAGCTGGC
    AGTGTTTCTC TGCGTATGAA TCAGGTTGAA GAATTGTATC ATAATATTTT GGAGATGGGA
    GAGAAGCGCA AAGATATGTT AGAAAAGAGC TGCAAGAAGT TTATGCTGTT CCGTGAGGCA
    AATGAGCTGC AGCAGTGGAT TAACGAGAAA GAAGCATCAC TCACTAACGA AGAGGTCGGG
    GCTGACCTGG AACAAGTGGA AGTGCTGCAG AAGAAGTTTG ATGATTTCCA GAAGGATCTG
    AAAGCAAATG AGTCACGCTT GAAGGACATC AACAAGGTGG CCGACGAGCT TGAATCTGAA
    GGCCTCATTA CAGAGGAAGT CCAGCCGGTG CAGCATCAGG AGGTGTATGG GATGCAGCGG
    GATGAAGCAG ACTCCAAATC GGCATCTCCA TGGAAGTCTG TTCGCACAGC TGTGCTCGCG
    GTGGCCACCT TCAACTCTAT CAAGGACTTG AACGAGCGCT GGAAATCACT TCAACAGCTG
    GCAGAAGAAA GGAGTCAGCT TCTTGGCAGT GCCCATGAAG TACAGAGATT CCACAGAGAT
    GCTGATGAGA CAAAAGAATG GATCGAAGAG AAGAACCAGG CTTTGAATAC AGACAGCTAT
    GGACATGACT TGGCCAGTGT GCAGGCTCTG CAACGAAAAC ATGAAGGCTT TGAGAGAGAC
    CTCGCCGCTC TGGGAGATAA GGTGAACTCT TTGGGAGAAA CTGCTGAGCG CCTGATCCAG
    TCACACCCTG AGTCTGCAGA TGACATTCAG GAAAAATGCA CAGAGCTGAA TCAGGCCTGG
    AACAGCCTTG GCACCCGGGC AAACCAGCGC AAGGAGAAAC TAGGCGATTC TCATGATCTG
    CAACGCTTCC TAAGTGACTT CAGAGATCTC ATGTCCTGGA TCAATGGAAT TCGGGGCTTG
    GTGTCATCAG ATGAACTTGC TAGAGATGTG ACTGGTGCAG AGGCTTTGCT GGAACGACAC
    CAGGAGCACA GGACTGAGAT TGATGCCAGA GCAGGCACTT TTCAGGCCTT TGAGCAGTTT
    GGGCAGCAAC TTCTGGCTCG TGGTCATTAT GATAGTCCTG AAATCAAGGA GAAGCTGGAT
    ATCTTGGAAC AGGAACGGGC AAGTTTGGAA AAGGCTTGGG TGGAACGCAG AATGATGTTG
    GATCAGTGCT TGGAGCTGCA GCTCTTCAAC AGAGACTGCG AGCAGGCAGA GAACTGGATG
    GCAGCCCGTG AAGCCTTCCT GAACAGTGAG GATAAAGGGG ATTCCCTGGA CAGTGTGGAG
    GCCCTTATCA AGAAACATGA AGACTTTGAT AAAGCCATTA ATGTCCAGGA AGAGAAGATT
    GCTGCCCTCC AGGCCTTCGC CGAACAATTG ATAATTGCTG ACCACTATGC CAAAGGAGAT
    ATTGCCTCCC GTCGCAATGA AGTCCTGGAC AGGTGGCGCC GTCTCAAGGC TCAGATGATT
    GAGAAGAGAT CCAAACTTGG GGAATCTCAG ACTCTGCAGC AGTTCAGCAG GGACGTCGAT
    GAGATCGAAG CCTGGATCAG CGAGAAATTG CAGACCGCCA GTGACGAGTC CTACAAAGAC
    CCCACAAACA TCCAGCTTTC CAAATTGCTG AGCAAACACC AGAAGCACCA GGCTTTCGAA
    GCTGAGCTCC ATGCCAACGC TGACCGTATT CGTGGAGTAG TAGATATGGG GAACTCCCTT
    ATCGAACGAG GTGCCTGTGC TGGCAGCGAG GATGCGGTGA AGGCTCGCCT AAGCGCACTC
    GCTGATCAAT GGCAGTTCCT TGTCAGCAAA TCCGCAGAGA AGAGCCAGAA ACTGAAGGAG
    GCAAACAAGC AGCAGAACTT TAACACTGGC ATCAAAGACT TTGATTTCTG GTTAACAGAG
    GTGGAGGCTT TGCTGGCATC TGAAGACTAT GGGAAGGACT TGGCATCTGT TAACAACCTG
    CTGAAGAAAC ACCAGCTTCT GGAAGCTGAC ATCTCTGCTC ATGAGGACCG CCTTAAAGAT
    CTTAATGGGC AGGCTGATAG CCTGATGACC AGCAGCGCCT TTGACACCAC CCAAGTAAAG
    GAGAAAAGGG ACGCCATCAA CGACCGCTTC CAGCGTATCA AAAGCATGGC CACCGCACGC
    CGCAGCAGGC TGAATGAGTC CCACCGTCTG CACCAGTTCT TCAGAGATCT TGATGATGAG
    GAATCATGGA TCAAGGAAAA GAAACTTTTG GTCGGATCTG ATGACTATGG ACGTGACTTG
    ACTGGAGTGC AAAATCTGAG GAAGAAACAC AAGAGATTAG AAGCTGAACT GGCAGCTCAT
    GAGCCAGCCA TTCAGGGTGT GCTGGACACT GGGAAGAAGC TTGCAGATGA CAACACTATT
    GGGAAAGATG AGATTCAGCA GCGACTGGCA CAGTTTCTAG AGCACTGGAA GGAGCTGAAG
    GAACAAGCAG CGGCTCGTGG TCAGAGACTG GAGGAATCTC TTGAGTACCA ACAATTTGTA
    GCCAATGTGG AGGAAGAGGA AGCCTGGATT AATGAGAAGA TGACTCTAGT GGCCAGTGAA
    GACTATGGTG ACACACTGGC TGCCATTCAG GGATTGCTGA AAAAGCACGA GGCATTTGAG
    ACAGACTTTA CAGTGCACAA AGACAGAGTA CATGATGTAT GTGCCAACGG GGAAGACCTG
    ATACAAAAGA ACAATCACCA TGTGGAGAAT ATTAGCGCCA AAATGAAGGG TCTCCGAGGG
    AAGGTGGCAG AACTAGAGAA GGCGGCTGCA CAGCGCAAGG CCAAACTTGA TGAGAATTCA
    GCTTTCCTGC AGTTCAACTG GAAGGCGGAT GTTGTGGAGT CCTGGATCGG TGAAAAAGAG
    AACAGCCTGA AGACTGATGA TTATGGCAGA GACCTGTCCT CTGTGCAGAC CCTTCTCACT
    AAGCAGGAAA CATTTGATGC TGGGCTGCAA GCTTTCCAGC AAGAGGGCAT TACCAACATC
    ACTGCACTGA AAGACCAGCT TCTGGCTGCA AAGCATGTGC AGTCCCGGGC CATAGAGTCT
    CGTCACGCTT CTCTGATGAA GCGGTGGAAC CAGTTGTTGG ATAACTCTGC TGCCCGCAAA
    AAGAAGTTAT TGGAGGCCCA GGAACACTAC AGAAAGGTTG AGGACTTGTT CCTAACATTT
    GCCAAGAAAG CCTCTGCCTT CAACAGCTGG TTTGAGAATG CCGAGGAGGA TTTGACTGAT
    CCTGTTCGCT GCAACTCCCT GGAGGAAATC AAGGCGCTTC GCGAGGCACA TGACGCCTTC
    CGCAATTCCC TCAGCTCCGC ACAGACAGAT TTCAACCAGC TGGCCGAACT GGACAGGCAA
    ATAAAGGGCT ACCGGGTAGC CTCCAATCCC TACACGTGGT TCACGATGGA GGCTTTGGAG
    GAGACATGGC GCAACCTGCA GAAAATCATT AAGGAACGAG AACTTGAGCT ACAAAAAGAA
    CAACGTAGGC AGGAAGAGAA CGATAAGCTC CGGCAAGAGT TTGCTCAGCA CGCCAATGCT
    TTCCACCAGT GGATTCAGGA GACAAGGACC TACCTTCTGG ACGGGTCCTG TATGGTGGAA
    GAATCTGGAA CACTGGAGTC ACAACTTGAA GCTACAAAGA GAAAGCATCA GGAGATCCGG
    GCCATGAGGA GTCAACTGAA GAAGATAGAA GATCTTGGCG CAGCCATGGA AGAGGCCCTG
    ATCTTGGATA ATAAATACAC AGAACACAGC ACTGTTGGGC TGGCACAGCA ATGGGACCAA
    CTGGATCAGC TAGGCATGAG AATGCAACAC AACCTAGAGC AACAGATCCA GGCCAGAAAC
    ACAACTGGGG TCACAGAGGA AGCACTCAAA GAATTCAGCA TGATGTTCAA GCATTTCGAC
    AAAGACAAGT CTGGCCGCCT CAACCACCAA GAATTCAAAT CTTGTCTGCG GTCCCTTGGC
    TATGATCTGC CCATGGTAGA AGAAGGAGAA CCAGACCCAG AGTTTGAAGC CATTTTGGAC
    ACTGTAGATC CCAACAGAGA TGGGCACGTT TCTCTACAGG AGTACATGGC TTTCATGATT
    AGCAGAGAAA CTGAAAACGT CAAGTCCAGT GAGGAGATAG AGAGCGCCTT CCGAGCACTG
    AGTACAGACC AGAAACCTTA TGTCACAAAG GAAGAACTAT ACCAGAACCT GACACGCGAA
    CAGGCAGATT ACTGCATCTC GCATATGAAA CCCTACATGG ACAGCAAGGG CCGCGAACTG
    CCCTCTGCAT ACGACTACGT GGAATTCACG CGCTCGCTAT TTGTGAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29835
    Clone ID Related Accession (Same CDS sequence) XM_031906806.1 , XM_012967794.2
    Accession Version XM_012967794.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7413bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012967794.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGATTCAG GTGGCGTGAA AGTGCTGGAG ACAGCGGAGG ACATCCAGGA AAGGCGCCAG 
    CAAGTCCTAG ACCGATACCT GCGGTTTAAG GAACTCTCTG TACTGAGACG TCAGAAGTTG
    GAGGATTCTT ACCGCTTCCA GTTCTTCCAG CGAGATGCTG ATGAACTGGA GAAATGGATC
    CAGGAGAAAC TCCAGATCGC ATCTGATGAG AACTACAAAG ATCCAACCAA TCTGCAGGGC
    AAATTGCAGA AACACCAGGC TTTTGAGGCA GAAGTACAGG CCAATGCAGG GGCAATTGTC
    AAACTGGACG AAACTGGAAA CCTGATGATT TCAGAAGGAC ATTTTTCATC AGAGACCATC
    AGAACCCGCC TGGTAGAGTT GCATCGTCTG TGGGAGCTGC TGCTCTTAAA GATGCAAGAG
    AAAGGGGTTA AATTACAACA GGCGCAGAAG CTTGTTCAGT ATCTACGGGA ATGTGAAGAT
    GTCCTAGACT GGATTAATGA CAAGGAGGCT ATTGTAACAT CTGAGGAGCT TGGTCAGGAT
    TTGGAACATG TGGAGGTCTT ACAGAAGAAG TTTGAAGAGT TTCAGACTGA TCTGGCAGCT
    CATGAAGAAA GAGTGAATGA AGTCAACCAG TTTGCAAGTA AACTGGCCCA GGAGGAACAC
    CCGGAGCTGG AGCTAATAAA GACCAAGCAG GATGAAGTCA ATGCAAGTTG GCAGCGCCTC
    AAAGGGCTGG CACTTCAGAG ACAAGGGAAG TTATTTGGAG CTGCAGAAGT TCAGCGTTTT
    AACAGAGATG TTGATGAAAC CATTGGATGG ATAAGAGAGA AGGAGCAGCT GATGGCATCT
    GATGACTTTG GCCGAGACCT GGCTAGCGTT CAAGCTCTCC TACGTAAACA CGAGGGTCTG
    GAGAGAGACC TTGCAGCACT GGAGGAAAAG GTAAAGGCCT TGTGTGCAGA ATCAGACCGT
    CTGCAGCAAT CCCATCCCAT GAACGCCCCT CAAATTCAAG TGAAAGGAGA AGAACTGATA
    ACCAACTGGG AGCAAATACG CACATTGGCA GCAGAACGAC ATGCTCGACT CAATGACTCT
    TACAAGCTTC AGCGTTTCCT TGCAGATTTT AGAGATCTGA CTAGCTGGGT GACTGAAATG
    AAAGCTCTGA TTAATGCCGA TGAGCTTGCC AACGATGTGG CTGGCGCAGA GGCTCTGCTT
    GACCGACACC AGGAACATAA GGGGGAAATT GATGCTCATG AAGATAGCTT TAAAGCTGCA
    GATGCTGCAG GTCAGGCCCT TCTGAACGGT GGCCACTACG CCTCAGATGA AGTCCGAGAA
    AAGCTTACTA TCTTAGCAGA AGAAAGGGCA GCTCTTCTAG AGCTTTGGGA GCTCCGTCGT
    CAGCAGTATG AACAGTGCAT GGACCTGCAG CTTTTTTACA GAGACACCGA ACAGGTGGAC
    AACTGGATGA GCAAACAGGA AGCTTTCCTG CTTAACGAGG ACTTGGGTGA TTCTCTAGAC
    AGTGTGGAAG CTCTTCTGAA AAAACATGAG GACTTTGAGA AGTCTCTTAG CGCCCAGGAA
    GAGAAAATCA CAGCTCTGGA TGAGTTTGCT ACAAAACTAA TTCAAAATAA CCACTATGCC
    AAGGAAGATG TGGCTACCCG CAGAGATGCT CTGCTGAGCC GCCGCAATGC ACTTCATGAA
    CGTGCACTCT ACCGCCGTAC TCAACTGGCA GACTCCTTTC ACCTGCAGCA GTTCTTTAGG
    GACTCTGACG AGCTGAAAAG TTGGATTAAT GAAAAGATGA AGACTGCAAC GGATGAGGCA
    TACAAGGATC CCTCCAACCT CCAGGGTAAG GTGCAGAAAC ACCAAGCTTT TGAGGCTGAA
    CTCTCCGCTA ACCAAAGCCG TATAGATGCC CTGGAGAATT CTGGGCAGAA ATTGATTGAT
    GTCAACCATT ATGCATCAGA TGAGGTGGCA GCACGTATGA GTGAGGTCAT CACTTTGTGG
    AAGAAGCTGC TGGAGGCCAC TGAATTGAAG GGGATCAAGC TGCGCGAAGC CAACCAGCAG
    CAGCAGTTCA ACCGTAATGT GGAGGATATA GAGCTGTGGC TGTATGAGGT TGAGGGGCAT
    TTGGCTTCAG ATGACTATGG AAAAGACCTG ACCAATGTTC AAAACTTGCA GAAAAAACAC
    GCCTTGCTGG AGGCTGATGT TGCAGCTCAC CAGGACCGTA TCGATGGCAT CACTATCCAA
    GCCCGTCAGT TCCAAGAAGC TGGTCATTTT GATGCTGACA ATATCAGGAA GAAACAGGAG
    GCCCTTGTTA ATAGATATGA AGCTTTGAAA GAACCCATGG TTGCACGCAA GCAGAAGCTG
    TCAGACTCTC TGCGTCTTCA ACAGCTTTTC AGGGATGTTG AAGATGAGGA AACCTGGATA
    AGGGAGAAGG AACCCATTGC AGCGTCCACC AACAGAGGCA AGGATTTGAT TGGAGTCCAG
    AATCTCCTGA AGAAACACCA GGCTTTGCAG GCTGAAATTG CAGGGCACGA ACCACGAATT
    AAAGCTGTTA CCCAGAAAGG AGAATCTATG ATCTCTGAAG GCCACTTTGC TTCTGATGAA
    GTGAAGGGCA AATTAAGGGA GCTGAATGAT AAGTGGGCCA CCCTGAAGAA CAAAGCATCT
    CAGCGCCGAC AGGACCTTGA AGATTCTCTG CAAGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCAGAGT CCTGGATGAG AGAGAAGGAA CCTATTGTTG GAAGCACCGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC CCTTCTGAAG AAACATGAAG CGCTTATGTC AGATCTGCGA
    GCATACGGAA GCAGTATTCA GGGACTTAGA GAGCAGGCCC AGGCCTGCAG GCAACAAGTT
    GCTCCAACTG ATGATGAAAC TGGAAAGGAG CTGGTGCTGG CATTGTATGA TTATCAAGAG
    AAGAGCCCTA GGGAGGTGAC AATGAAGAAA GGAGACATCT TGACCTTGCT AAACAGCACA
    AATAAGGATT GGTGGAAGGT GGAAGTGAAT GATCGTCAGG GCTTTGTACC TGCTGCGTAT
    GTGAAAAAGC TGGACCCAGC ACAGTCCGCA TCCCGGGAAA ACTTGCTGGA GGAACAGGGA
    AGCATCGCTC TCCGTCAGGA GCAGATCGAT GGCCAGGCTC ACATGTCTAA GGAAGCTGGC
    AGTGTTTCTC TGCGTATGAA TCAGGTTGAA GAATTGTATC ATAATATTTT GGAGATGGGA
    GAGAAGCGCA AAGATATGTT AGAAAAGAGC TGCAAGAAGT TTATGCTGTT CCGTGAGGCA
    AATGAGCTGC AGCAGTGGAT TAACGAGAAA GAAGCATCAC TCACTAACGA AGAGGTCGGG
    GCTGACCTGG AACAAGTGGA AGTGCTGCAG AAGAAGTTTG ATGATTTCCA GAAGGATCTG
    AAAGCAAATG AGTCACGCTT GAAGGACATC AACAAGGTGG CCGACGAGCT TGAATCTGAA
    GGCCTCATTA CAGAGGAAGT CCAGCCGGTG CAGCATCAGG AGGTGTATGG GATGCAGCGG
    GATGAAGCAG ACTCCAAATC GGCATCTCCA TGGAAGTCTG TTCGCACAGC TGTGCTCGCG
    GTGGCCACCT TCAACTCTAT CAAGGACTTG AACGAGCGCT GGAAATCACT TCAACAGCTG
    GCAGAAGAAA GGAGTCAGCT TCTTGGCAGT GCCCATGAAG TACAGAGATT CCACAGAGAT
    GCTGATGAGA CAAAAGAATG GATCGAAGAG AAGAACCAGG CTTTGAATAC AGACAGCTAT
    GGACATGACT TGGCCAGTGT GCAGGCTCTG CAACGAAAAC ATGAAGGCTT TGAGAGAGAC
    CTCGCCGCTC TGGGAGATAA GGTGAACTCT TTGGGAGAAA CTGCTGAGCG CCTGATCCAG
    TCACACCCTG AGTCTGCAGA TGACATTCAG GAAAAATGCA CAGAGCTGAA TCAGGCCTGG
    AACAGCCTTG GCACCCGGGC AAACCAGCGC AAGGAGAAAC TAGGCGATTC TCATGATCTG
    CAACGCTTCC TAAGTGACTT CAGAGATCTC ATGTCCTGGA TCAATGGAAT TCGGGGCTTG
    GTGTCATCAG ATGAACTTGC TAGAGATGTG ACTGGTGCAG AGGCTTTGCT GGAACGACAC
    CAGGAGCACA GGACTGAGAT TGATGCCAGA GCAGGCACTT TTCAGGCCTT TGAGCAGTTT
    GGGCAGCAAC TTCTGGCTCG TGGTCATTAT GATAGTCCTG AAATCAAGGA GAAGCTGGAT
    ATCTTGGAAC AGGAACGGGC AAGTTTGGAA AAGGCTTGGG TGGAACGCAG AATGATGTTG
    GATCAGTGCT TGGAGCTGCA GCTCTTCAAC AGAGACTGCG AGCAGGCAGA GAACTGGATG
    GCAGCCCGTG AAGCCTTCCT GAACAGTGAG GATAAAGGGG ATTCCCTGGA CAGTGTGGAG
    GCCCTTATCA AGAAACATGA AGACTTTGAT AAAGCCATTA ATGTCCAGGA AGAGAAGATT
    GCTGCCCTCC AGGCCTTCGC CGAACAATTG ATAATTGCTG ACCACTATGC CAAAGGAGAT
    ATTGCCTCCC GTCGCAATGA AGTCCTGGAC AGGTGGCGCC GTCTCAAGGC TCAGATGATT
    GAGAAGAGAT CCAAACTTGG GGAATCTCAG ACTCTGCAGC AGTTCAGCAG GGACGTCGAT
    GAGATCGAAG CCTGGATCAG CGAGAAATTG CAGACCGCCA GTGACGAGTC CTACAAAGAC
    CCCACAAACA TCCAGCTTTC CAAATTGCTG AGCAAACACC AGAAGCACCA GGCTTTCGAA
    GCTGAGCTCC ATGCCAACGC TGACCGTATT CGTGGAGTAG TAGATATGGG GAACTCCCTT
    ATCGAACGAG GTGCCTGTGC TGGCAGCGAG GATGCGGTGA AGGCTCGCCT AAGCGCACTC
    GCTGATCAAT GGCAGTTCCT TGTCAGCAAA TCCGCAGAGA AGAGCCAGAA ACTGAAGGAG
    GCAAACAAGC AGCAGAACTT TAACACTGGC ATCAAAGACT TTGATTTCTG GTTAACAGAG
    GTGGAGGCTT TGCTGGCATC TGAAGACTAT GGGAAGGACT TGGCATCTGT TAACAACCTG
    CTGAAGAAAC ACCAGCTTCT GGAAGCTGAC ATCTCTGCTC ATGAGGACCG CCTTAAAGAT
    CTTAATGGGC AGGCTGATAG CCTGATGACC AGCAGCGCCT TTGACACCAC CCAAGTAAAG
    GAGAAAAGGG ACGCCATCAA CGACCGCTTC CAGCGTATCA AAAGCATGGC CACCGCACGC
    CGCAGCAGGC TGAATGAGTC CCACCGTCTG CACCAGTTCT TCAGAGATCT TGATGATGAG
    GAATCATGGA TCAAGGAAAA GAAACTTTTG GTCGGATCTG ATGACTATGG ACGTGACTTG
    ACTGGAGTGC AAAATCTGAG GAAGAAACAC AAGAGATTAG AAGCTGAACT GGCAGCTCAT
    GAGCCAGCCA TTCAGGGTGT GCTGGACACT GGGAAGAAGC TTGCAGATGA CAACACTATT
    GGGAAAGATG AGATTCAGCA GCGACTGGCA CAGTTTCTAG AGCACTGGAA GGAGCTGAAG
    GAACAAGCAG CGGCTCGTGG TCAGAGACTG GAGGAATCTC TTGAGTACCA ACAATTTGTA
    GCCAATGTGG AGGAAGAGGA AGCCTGGATT AATGAGAAGA TGACTCTAGT GGCCAGTGAA
    GACTATGGTG ACACACTGGC TGCCATTCAG GGATTGCTGA AAAAGCACGA GGCATTTGAG
    ACAGACTTTA CAGTGCACAA AGACAGAGTA CATGATGTAT GTGCCAACGG GGAAGACCTG
    ATACAAAAGA ACAATCACCA TGTGGAGAAT ATTAGCGCCA AAATGAAGGG TCTCCGAGGG
    AAGGTGGCAG AACTAGAGAA GGCGGCTGCA CAGCGCAAGG CCAAACTTGA TGAGAATTCA
    GCTTTCCTGC AGTTCAACTG GAAGGCGGAT GTTGTGGAGT CCTGGATCGG TGAAAAAGAG
    AACAGCCTGA AGACTGATGA TTATGGCAGA GACCTGTCCT CTGTGCAGAC CCTTCTCACT
    AAGCAGGAAA CATTTGATGC TGGGCTGCAA GCTTTCCAGC AAGAGGGCAT TACCAACATC
    ACTGCACTGA AAGACCAGCT TCTGGCTGCA AAGCATGTGC AGTCCCGGGC CATAGAGTCT
    CGTCACGCTT CTCTGATGAA GCGGTGGAAC CAGTTGTTGG ATAACTCTGC TGCCCGCAAA
    AAGAAGTTAT TGGAGGCCCA GGAACACTAC AGAAAGGTTG AGGACTTGTT CCTAACATTT
    GCCAAGAAAG CCTCTGCCTT CAACAGCTGG TTTGAGAATG CCGAGGAGGA TTTGACTGAT
    CCTGTTCGCT GCAACTCCCT GGAGGAAATC AAGGCGCTTC GCGAGGCACA TGACGCCTTC
    CGCAATTCCC TCAGCTCCGC ACAGACAGAT TTCAACCAGC TGGCCGAACT GGACAGGCAA
    ATAAAGGGCT ACCGGGTAGC CTCCAATCCC TACACGTGGT TCACGATGGA GGCTTTGGAG
    GAGACATGGC GCAACCTGCA GAAAATCATT AAGGAACGAG AACTTGAGCT ACAAAAAGAA
    CAACGTAGGC AGGAAGAGAA CGATAAGCTC CGGCAAGAGT TTGCTCAGCA CGCCAATGCT
    TTCCACCAGT GGATTCAGGA GACAAGGTCC TGTATGGTGG AAGAATCTGG AACACTGGAG
    TCACAACTTG AAGCTACAAA GAGAAAGCAT CAGGAGATCC GGGCCATGAG GAGTCAACTG
    AAGAAGATAG AAGATCTTGG CGCAGCCATG GAAGAGGCCC TGATCTTGGA TAATAAATAC
    ACAGAACACA GCACTGTTGG GCTGGCACAG CAATGGGACC AACTGGATCA GCTAGGCATG
    AGAATGCAAC ACAACCTAGA GCAACAGATC CAGGCCAGAA ACACAACTGG GGTCACAGAG
    GAAGCACTCA AAGAATTCAG CATGATGTTC AAGCATTTCG ACAAAGACAA GTCTGGCCGC
    CTCAACCACC AAGAATTCAA ATCTTGTCTG CGGTCCCTTG GCTATGATCT GCCCATGGTA
    GAAGAAGGAG AACCAGACCC AGAGTTTGAA GCCATTTTGG ACACTGTAGA TCCCAACAGA
    GATGGGCACG TTTCTCTACA GGAGTACATG GCTTTCATGA TTAGCAGAGA AACTGAAAAC
    GTCAAGTCCA GTGAGGAGAT AGAGAGCGCC TTCCGAGCAC TGAGTACAGA CCAGAAACCT
    TATGTCACAA AGGAAGAACT ATACCAGAAC CTGACACGCG AACAGGCAGA TTACTGCATC
    TCGCATATGA AACCCTACAT GGACAGCAAG GGCCGCGAAC TGCCCTCTGC ATACGACTAC
    GTGGAATTCA CGCGCTCGCT ATTTGTGAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012823248.1
    CDS265..7677
    Translation

    Target ORF information:

    RefSeq Version XM_012967794.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis spectrin, alpha, non-erythrocytic 1 (sptan1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012967794.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGATTCAG GTGGCGTGAA AGTGCTGGAG ACAGCGGAGG ACATCCAGGA AAGGCGCCAG 
    CAAGTCCTAG ACCGATACCT GCGGTTTAAG GAACTCTCTG TACTGAGACG TCAGAAGTTG
    GAGGATTCTT ACCGCTTCCA GTTCTTCCAG CGAGATGCTG ATGAACTGGA GAAATGGATC
    CAGGAGAAAC TCCAGATCGC ATCTGATGAG AACTACAAAG ATCCAACCAA TCTGCAGGGC
    AAATTGCAGA AACACCAGGC TTTTGAGGCA GAAGTACAGG CCAATGCAGG GGCAATTGTC
    AAACTGGACG AAACTGGAAA CCTGATGATT TCAGAAGGAC ATTTTTCATC AGAGACCATC
    AGAACCCGCC TGGTAGAGTT GCATCGTCTG TGGGAGCTGC TGCTCTTAAA GATGCAAGAG
    AAAGGGGTTA AATTACAACA GGCGCAGAAG CTTGTTCAGT ATCTACGGGA ATGTGAAGAT
    GTCCTAGACT GGATTAATGA CAAGGAGGCT ATTGTAACAT CTGAGGAGCT TGGTCAGGAT
    TTGGAACATG TGGAGGTCTT ACAGAAGAAG TTTGAAGAGT TTCAGACTGA TCTGGCAGCT
    CATGAAGAAA GAGTGAATGA AGTCAACCAG TTTGCAAGTA AACTGGCCCA GGAGGAACAC
    CCGGAGCTGG AGCTAATAAA GACCAAGCAG GATGAAGTCA ATGCAAGTTG GCAGCGCCTC
    AAAGGGCTGG CACTTCAGAG ACAAGGGAAG TTATTTGGAG CTGCAGAAGT TCAGCGTTTT
    AACAGAGATG TTGATGAAAC CATTGGATGG ATAAGAGAGA AGGAGCAGCT GATGGCATCT
    GATGACTTTG GCCGAGACCT GGCTAGCGTT CAAGCTCTCC TACGTAAACA CGAGGGTCTG
    GAGAGAGACC TTGCAGCACT GGAGGAAAAG GTAAAGGCCT TGTGTGCAGA ATCAGACCGT
    CTGCAGCAAT CCCATCCCAT GAACGCCCCT CAAATTCAAG TGAAAGGAGA AGAACTGATA
    ACCAACTGGG AGCAAATACG CACATTGGCA GCAGAACGAC ATGCTCGACT CAATGACTCT
    TACAAGCTTC AGCGTTTCCT TGCAGATTTT AGAGATCTGA CTAGCTGGGT GACTGAAATG
    AAAGCTCTGA TTAATGCCGA TGAGCTTGCC AACGATGTGG CTGGCGCAGA GGCTCTGCTT
    GACCGACACC AGGAACATAA GGGGGAAATT GATGCTCATG AAGATAGCTT TAAAGCTGCA
    GATGCTGCAG GTCAGGCCCT TCTGAACGGT GGCCACTACG CCTCAGATGA AGTCCGAGAA
    AAGCTTACTA TCTTAGCAGA AGAAAGGGCA GCTCTTCTAG AGCTTTGGGA GCTCCGTCGT
    CAGCAGTATG AACAGTGCAT GGACCTGCAG CTTTTTTACA GAGACACCGA ACAGGTGGAC
    AACTGGATGA GCAAACAGGA AGCTTTCCTG CTTAACGAGG ACTTGGGTGA TTCTCTAGAC
    AGTGTGGAAG CTCTTCTGAA AAAACATGAG GACTTTGAGA AGTCTCTTAG CGCCCAGGAA
    GAGAAAATCA CAGCTCTGGA TGAGTTTGCT ACAAAACTAA TTCAAAATAA CCACTATGCC
    AAGGAAGATG TGGCTACCCG CAGAGATGCT CTGCTGAGCC GCCGCAATGC ACTTCATGAA
    CGTGCACTCT ACCGCCGTAC TCAACTGGCA GACTCCTTTC ACCTGCAGCA GTTCTTTAGG
    GACTCTGACG AGCTGAAAAG TTGGATTAAT GAAAAGATGA AGACTGCAAC GGATGAGGCA
    TACAAGGATC CCTCCAACCT CCAGGGTAAG GTGCAGAAAC ACCAAGCTTT TGAGGCTGAA
    CTCTCCGCTA ACCAAAGCCG TATAGATGCC CTGGAGAATT CTGGGCAGAA ATTGATTGAT
    GTCAACCATT ATGCATCAGA TGAGGTGGCA GCACGTATGA GTGAGGTCAT CACTTTGTGG
    AAGAAGCTGC TGGAGGCCAC TGAATTGAAG GGGATCAAGC TGCGCGAAGC CAACCAGCAG
    CAGCAGTTCA ACCGTAATGT GGAGGATATA GAGCTGTGGC TGTATGAGGT TGAGGGGCAT
    TTGGCTTCAG ATGACTATGG AAAAGACCTG ACCAATGTTC AAAACTTGCA GAAAAAACAC
    GCCTTGCTGG AGGCTGATGT TGCAGCTCAC CAGGACCGTA TCGATGGCAT CACTATCCAA
    GCCCGTCAGT TCCAAGAAGC TGGTCATTTT GATGCTGACA ATATCAGGAA GAAACAGGAG
    GCCCTTGTTA ATAGATATGA AGCTTTGAAA GAACCCATGG TTGCACGCAA GCAGAAGCTG
    TCAGACTCTC TGCGTCTTCA ACAGCTTTTC AGGGATGTTG AAGATGAGGA AACCTGGATA
    AGGGAGAAGG AACCCATTGC AGCGTCCACC AACAGAGGCA AGGATTTGAT TGGAGTCCAG
    AATCTCCTGA AGAAACACCA GGCTTTGCAG GCTGAAATTG CAGGGCACGA ACCACGAATT
    AAAGCTGTTA CCCAGAAAGG AGAATCTATG ATCTCTGAAG GCCACTTTGC TTCTGATGAA
    GTGAAGGGCA AATTAAGGGA GCTGAATGAT AAGTGGGCCA CCCTGAAGAA CAAAGCATCT
    CAGCGCCGAC AGGACCTTGA AGATTCTCTG CAAGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCAGAGT CCTGGATGAG AGAGAAGGAA CCTATTGTTG GAAGCACCGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC CCTTCTGAAG AAACATGAAG CGCTTATGTC AGATCTGCGA
    GCATACGGAA GCAGTATTCA GGGACTTAGA GAGCAGGCCC AGGCCTGCAG GCAACAAGTT
    GCTCCAACTG ATGATGAAAC TGGAAAGGAG CTGGTGCTGG CATTGTATGA TTATCAAGAG
    AAGAGCCCTA GGGAGGTGAC AATGAAGAAA GGAGACATCT TGACCTTGCT AAACAGCACA
    AATAAGGATT GGTGGAAGGT GGAAGTGAAT GATCGTCAGG GCTTTGTACC TGCTGCGTAT
    GTGAAAAAGC TGGACCCAGC ACAGTCCGCA TCCCGGGAAA ACTTGCTGGA GGAACAGGGA
    AGCATCGCTC TCCGTCAGGA GCAGATCGAT GGCCAGGCTC ACATGTCTAA GGAAGCTGGC
    AGTGTTTCTC TGCGTATGAA TCAGGTTGAA GAATTGTATC ATAATATTTT GGAGATGGGA
    GAGAAGCGCA AAGATATGTT AGAAAAGAGC TGCAAGAAGT TTATGCTGTT CCGTGAGGCA
    AATGAGCTGC AGCAGTGGAT TAACGAGAAA GAAGCATCAC TCACTAACGA AGAGGTCGGG
    GCTGACCTGG AACAAGTGGA AGTGCTGCAG AAGAAGTTTG ATGATTTCCA GAAGGATCTG
    AAAGCAAATG AGTCACGCTT GAAGGACATC AACAAGGTGG CCGACGAGCT TGAATCTGAA
    GGCCTCATTA CAGAGGAAGT CCAGCCGGTG CAGCATCAGG AGGTGTATGG GATGCAGCGG
    GATGAAGCAG ACTCCAAATC GGCATCTCCA TGGAAGTCTG TTCGCACAGC TGTGCTCGCG
    GTGGCCACCT TCAACTCTAT CAAGGACTTG AACGAGCGCT GGAAATCACT TCAACAGCTG
    GCAGAAGAAA GGAGTCAGCT TCTTGGCAGT GCCCATGAAG TACAGAGATT CCACAGAGAT
    GCTGATGAGA CAAAAGAATG GATCGAAGAG AAGAACCAGG CTTTGAATAC AGACAGCTAT
    GGACATGACT TGGCCAGTGT GCAGGCTCTG CAACGAAAAC ATGAAGGCTT TGAGAGAGAC
    CTCGCCGCTC TGGGAGATAA GGTGAACTCT TTGGGAGAAA CTGCTGAGCG CCTGATCCAG
    TCACACCCTG AGTCTGCAGA TGACATTCAG GAAAAATGCA CAGAGCTGAA TCAGGCCTGG
    AACAGCCTTG GCACCCGGGC AAACCAGCGC AAGGAGAAAC TAGGCGATTC TCATGATCTG
    CAACGCTTCC TAAGTGACTT CAGAGATCTC ATGTCCTGGA TCAATGGAAT TCGGGGCTTG
    GTGTCATCAG ATGAACTTGC TAGAGATGTG ACTGGTGCAG AGGCTTTGCT GGAACGACAC
    CAGGAGCACA GGACTGAGAT TGATGCCAGA GCAGGCACTT TTCAGGCCTT TGAGCAGTTT
    GGGCAGCAAC TTCTGGCTCG TGGTCATTAT GATAGTCCTG AAATCAAGGA GAAGCTGGAT
    ATCTTGGAAC AGGAACGGGC AAGTTTGGAA AAGGCTTGGG TGGAACGCAG AATGATGTTG
    GATCAGTGCT TGGAGCTGCA GCTCTTCAAC AGAGACTGCG AGCAGGCAGA GAACTGGATG
    GCAGCCCGTG AAGCCTTCCT GAACAGTGAG GATAAAGGGG ATTCCCTGGA CAGTGTGGAG
    GCCCTTATCA AGAAACATGA AGACTTTGAT AAAGCCATTA ATGTCCAGGA AGAGAAGATT
    GCTGCCCTCC AGGCCTTCGC CGAACAATTG ATAATTGCTG ACCACTATGC CAAAGGAGAT
    ATTGCCTCCC GTCGCAATGA AGTCCTGGAC AGGTGGCGCC GTCTCAAGGC TCAGATGATT
    GAGAAGAGAT CCAAACTTGG GGAATCTCAG ACTCTGCAGC AGTTCAGCAG GGACGTCGAT
    GAGATCGAAG CCTGGATCAG CGAGAAATTG CAGACCGCCA GTGACGAGTC CTACAAAGAC
    CCCACAAACA TCCAGCTTTC CAAATTGCTG AGCAAACACC AGAAGCACCA GGCTTTCGAA
    GCTGAGCTCC ATGCCAACGC TGACCGTATT CGTGGAGTAG TAGATATGGG GAACTCCCTT
    ATCGAACGAG GTGCCTGTGC TGGCAGCGAG GATGCGGTGA AGGCTCGCCT AAGCGCACTC
    GCTGATCAAT GGCAGTTCCT TGTCAGCAAA TCCGCAGAGA AGAGCCAGAA ACTGAAGGAG
    GCAAACAAGC AGCAGAACTT TAACACTGGC ATCAAAGACT TTGATTTCTG GTTAACAGAG
    GTGGAGGCTT TGCTGGCATC TGAAGACTAT GGGAAGGACT TGGCATCTGT TAACAACCTG
    CTGAAGAAAC ACCAGCTTCT GGAAGCTGAC ATCTCTGCTC ATGAGGACCG CCTTAAAGAT
    CTTAATGGGC AGGCTGATAG CCTGATGACC AGCAGCGCCT TTGACACCAC CCAAGTAAAG
    GAGAAAAGGG ACGCCATCAA CGACCGCTTC CAGCGTATCA AAAGCATGGC CACCGCACGC
    CGCAGCAGGC TGAATGAGTC CCACCGTCTG CACCAGTTCT TCAGAGATCT TGATGATGAG
    GAATCATGGA TCAAGGAAAA GAAACTTTTG GTCGGATCTG ATGACTATGG ACGTGACTTG
    ACTGGAGTGC AAAATCTGAG GAAGAAACAC AAGAGATTAG AAGCTGAACT GGCAGCTCAT
    GAGCCAGCCA TTCAGGGTGT GCTGGACACT GGGAAGAAGC TTGCAGATGA CAACACTATT
    GGGAAAGATG AGATTCAGCA GCGACTGGCA CAGTTTCTAG AGCACTGGAA GGAGCTGAAG
    GAACAAGCAG CGGCTCGTGG TCAGAGACTG GAGGAATCTC TTGAGTACCA ACAATTTGTA
    GCCAATGTGG AGGAAGAGGA AGCCTGGATT AATGAGAAGA TGACTCTAGT GGCCAGTGAA
    GACTATGGTG ACACACTGGC TGCCATTCAG GGATTGCTGA AAAAGCACGA GGCATTTGAG
    ACAGACTTTA CAGTGCACAA AGACAGAGTA CATGATGTAT GTGCCAACGG GGAAGACCTG
    ATACAAAAGA ACAATCACCA TGTGGAGAAT ATTAGCGCCA AAATGAAGGG TCTCCGAGGG
    AAGGTGGCAG AACTAGAGAA GGCGGCTGCA CAGCGCAAGG CCAAACTTGA TGAGAATTCA
    GCTTTCCTGC AGTTCAACTG GAAGGCGGAT GTTGTGGAGT CCTGGATCGG TGAAAAAGAG
    AACAGCCTGA AGACTGATGA TTATGGCAGA GACCTGTCCT CTGTGCAGAC CCTTCTCACT
    AAGCAGGAAA CATTTGATGC TGGGCTGCAA GCTTTCCAGC AAGAGGGCAT TACCAACATC
    ACTGCACTGA AAGACCAGCT TCTGGCTGCA AAGCATGTGC AGTCCCGGGC CATAGAGTCT
    CGTCACGCTT CTCTGATGAA GCGGTGGAAC CAGTTGTTGG ATAACTCTGC TGCCCGCAAA
    AAGAAGTTAT TGGAGGCCCA GGAACACTAC AGAAAGGTTG AGGACTTGTT CCTAACATTT
    GCCAAGAAAG CCTCTGCCTT CAACAGCTGG TTTGAGAATG CCGAGGAGGA TTTGACTGAT
    CCTGTTCGCT GCAACTCCCT GGAGGAAATC AAGGCGCTTC GCGAGGCACA TGACGCCTTC
    CGCAATTCCC TCAGCTCCGC ACAGACAGAT TTCAACCAGC TGGCCGAACT GGACAGGCAA
    ATAAAGGGCT ACCGGGTAGC CTCCAATCCC TACACGTGGT TCACGATGGA GGCTTTGGAG
    GAGACATGGC GCAACCTGCA GAAAATCATT AAGGAACGAG AACTTGAGCT ACAAAAAGAA
    CAACGTAGGC AGGAAGAGAA CGATAAGCTC CGGCAAGAGT TTGCTCAGCA CGCCAATGCT
    TTCCACCAGT GGATTCAGGA GACAAGGTCC TGTATGGTGG AAGAATCTGG AACACTGGAG
    TCACAACTTG AAGCTACAAA GAGAAAGCAT CAGGAGATCC GGGCCATGAG GAGTCAACTG
    AAGAAGATAG AAGATCTTGG CGCAGCCATG GAAGAGGCCC TGATCTTGGA TAATAAATAC
    ACAGAACACA GCACTGTTGG GCTGGCACAG CAATGGGACC AACTGGATCA GCTAGGCATG
    AGAATGCAAC ACAACCTAGA GCAACAGATC CAGGCCAGAA ACACAACTGG GGTCACAGAG
    GAAGCACTCA AAGAATTCAG CATGATGTTC AAGCATTTCG ACAAAGACAA GTCTGGCCGC
    CTCAACCACC AAGAATTCAA ATCTTGTCTG CGGTCCCTTG GCTATGATCT GCCCATGGTA
    GAAGAAGGAG AACCAGACCC AGAGTTTGAA GCCATTTTGG ACACTGTAGA TCCCAACAGA
    GATGGGCACG TTTCTCTACA GGAGTACATG GCTTTCATGA TTAGCAGAGA AACTGAAAAC
    GTCAAGTCCA GTGAGGAGAT AGAGAGCGCC TTCCGAGCAC TGAGTACAGA CCAGAAACCT
    TATGTCACAA AGGAAGAACT ATACCAGAAC CTGACACGCG AACAGGCAGA TTACTGCATC
    TCGCATATGA AACCCTACAT GGACAGCAAG GGCCGCGAAC TGCCCTCTGC ATACGACTAC
    GTGGAATTCA CGCGCTCGCT ATTTGTGAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29836
    Clone ID Related Accession (Same CDS sequence) XM_031906807.1 , XM_012967795.2
    Accession Version XM_012967795.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7371bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012967795.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGGATTCAG GTGGCGTGAA AGTGCTGGAG ACAGCGGAGG ACATCCAGGA AAGGCGCCAG 
    CAAGTCCTAG ACCGATACCT GCGGTTTAAG GAACTCTCTG TACTGAGACG TCAGAAGTTG
    GAGGATTCTT ACCGCTTCCA GTTCTTCCAG CGAGATGCTG ATGAACTGGA GAAATGGATC
    CAGGAGAAAC TCCAGATCGC ATCTGATGAG AACTACAAAG ATCCAACCAA TCTGCAGGGC
    AAATTGCAGA AACACCAGGC TTTTGAGGCA GAAGTACAGG CCAATGCAGG GGCAATTGTC
    AAACTGGACG AAACTGGAAA CCTGATGATT TCAGAAGGAC ATTTTTCATC AGAGACCATC
    AGAACCCGCC TGGTAGAGTT GCATCGTCTG TGGGAGCTGC TGCTCTTAAA GATGCAAGAG
    AAAGGGGTTA AATTACAACA GGCGCAGAAG CTTGTTCAGT ATCTACGGGA ATGTGAAGAT
    GTCCTAGACT GGATTAATGA CAAGGAGGCT ATTGTAACAT CTGAGGAGCT TGGTCAGGAT
    TTGGAACATG TGGAGGTCTT ACAGAAGAAG TTTGAAGAGT TTCAGACTGA TCTGGCAGCT
    CATGAAGAAA GAGTGAATGA AGTCAACCAG TTTGCAAGTA AACTGGCCCA GGAGGAACAC
    CCGGAGCTGG AGCTAATAAA GACCAAGCAG GATGAAGTCA ATGCAAGTTG GCAGCGCCTC
    AAAGGGCTGG CACTTCAGAG ACAAGGGAAG TTATTTGGAG CTGCAGAAGT TCAGCGTTTT
    AACAGAGATG TTGATGAAAC CATTGGATGG ATAAGAGAGA AGGAGCAGCT GATGGCATCT
    GATGACTTTG GCCGAGACCT GGCTAGCGTT CAAGCTCTCC TACGTAAACA CGAGGGTCTG
    GAGAGAGACC TTGCAGCACT GGAGGAAAAG GTAAAGGCCT TGTGTGCAGA ATCAGACCGT
    CTGCAGCAAT CCCATCCCAT GAACGCCCCT CAAATTCAAG TGAAAGGAGA AGAACTGATA
    ACCAACTGGG AGCAAATACG CACATTGGCA GCAGAACGAC ATGCTCGACT CAATGACTCT
    TACAAGCTTC AGCGTTTCCT TGCAGATTTT AGAGATCTGA CTAGCTGGGT GACTGAAATG
    AAAGCTCTGA TTAATGCCGA TGAGCTTGCC AACGATGTGG CTGGCGCAGA GGCTCTGCTT
    GACCGACACC AGGAACATAA GGGGGAAATT GATGCTCATG AAGATAGCTT TAAAGCTGCA
    GATGCTGCAG GTCAGGCCCT TCTGAACGGT GGCCACTACG CCTCAGATGA AGTCCGAGAA
    AAGCTTACTA TCTTAGCAGA AGAAAGGGCA GCTCTTCTAG AGCTTTGGGA GCTCCGTCGT
    CAGCAGTATG AACAGTGCAT GGACCTGCAG CTTTTTTACA GAGACACCGA ACAGGTGGAC
    AACTGGATGA GCAAACAGGA AGCTTTCCTG CTTAACGAGG ACTTGGGTGA TTCTCTAGAC
    AGTGTGGAAG CTCTTCTGAA AAAACATGAG GACTTTGAGA AGTCTCTTAG CGCCCAGGAA
    GAGAAAATCA CAGCTCTGGA TGAGTTTGCT ACAAAACTAA TTCAAAATAA CCACTATGCC
    AAGGAAGATG TGGCTACCCG CAGAGATGCT CTGCTGAGCC GCCGCAATGC ACTTCATGAA
    CGTGCACTCT ACCGCCGTAC TCAACTGGCA GACTCCTTTC ACCTGCAGCA GTTCTTTAGG
    GACTCTGACG AGCTGAAAAG TTGGATTAAT GAAAAGATGA AGACTGCAAC GGATGAGGCA
    TACAAGGATC CCTCCAACCT CCAGGGTAAG GTGCAGAAAC ACCAAGCTTT TGAGGCTGAA
    CTCTCCGCTA ACCAAAGCCG TATAGATGCC CTGGAGAATT CTGGGCAGAA ATTGATTGAT
    GTCAACCATT ATGCATCAGA TGAGGTGGCA GCACGTATGA GTGAGGTCAT CACTTTGTGG
    AAGAAGCTGC TGGAGGCCAC TGAATTGAAG GGGATCAAGC TGCGCGAAGC CAACCAGCAG
    CAGCAGTTCA ACCGTAATGT GGAGGATATA GAGCTGTGGC TGTATGAGGT TGAGGGGCAT
    TTGGCTTCAG ATGACTATGG AAAAGACCTG ACCAATGTTC AAAACTTGCA GAAAAAACAC
    GCCTTGCTGG AGGCTGATGT TGCAGCTCAC CAGGACCGTA TCGATGGCAT CACTATCCAA
    GCCCGTCAGT TCCAAGAAGC TGGTCATTTT GATGCTGACA ATATCAGGAA GAAACAGGAG
    GCCCTTGTTA ATAGATATGA AGCTTTGAAA GAACCCATGG TTGCACGCAA GCAGAAGCTG
    TCAGACTCTC TGCGTCTTCA ACAGCTTTTC AGGGATGTTG AAGATGAGGA AACCTGGATA
    AGGGAGAAGG AACCCATTGC AGCGTCCACC AACAGAGGCA AGGATTTGAT TGGAGTCCAG
    AATCTCCTGA AGAAACACCA GGCTTTGCAG GCTGAAATTG CAGGGCACGA ACCACGAATT
    AAAGCTGTTA CCCAGAAAGG AGAATCTATG ATCTCTGAAG GCCACTTTGC TTCTGATGAA
    GTGAAGGGCA AATTAAGGGA GCTGAATGAT AAGTGGGCCA CCCTGAAGAA CAAAGCATCT
    CAGCGCCGAC AGGACCTTGA AGATTCTCTG CAAGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCAGAGT CCTGGATGAG AGAGAAGGAA CCTATTGTTG GAAGCACCGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC CCTTCTGAAG AAACATGAAG CGCTTATGTC AGATCTGCGA
    GCATACGGAA GCAGTATTCA GGGACTTAGA GAGCAGGCCC AGGCCTGCAG GCAACAAGTT
    GCTCCAACTG ATGATGAAAC TGGAAAGGAG CTGGTGCTGG CATTGTATGA TTATCAAGAG
    AAGAGCCCTA GGGAGGTGAC AATGAAGAAA GGAGACATCT TGACCTTGCT AAACAGCACA
    AATAAGGATT GGTGGAAGGT GGAAGTGAAT GATCGTCAGG GCTTTGTACC TGCTGCGTAT
    GTGAAAAAGC TGGACCCAGC ACAGTCCGCA TCCCGGGAAA ACTTGCTGGA GGAACAGGGA
    AGCATCGCTC TCCGTCAGGA GCAGATCGAT GGCCAGTATC ATAATATTTT GGAGATGGGA
    GAGAAGCGCA AAGATATGTT AGAAAAGAGC TGCAAGAAGT TTATGCTGTT CCGTGAGGCA
    AATGAGCTGC AGCAGTGGAT TAACGAGAAA GAAGCATCAC TCACTAACGA AGAGGTCGGG
    GCTGACCTGG AACAAGTGGA AGTGCTGCAG AAGAAGTTTG ATGATTTCCA GAAGGATCTG
    AAAGCAAATG AGTCACGCTT GAAGGACATC AACAAGGTGG CCGACGAGCT TGAATCTGAA
    GGCCTCATTA CAGAGGAAGT CCAGCCGGTG CAGCATCAGG AGGTGTATGG GATGCAGCGG
    GATGAAGCAG ACTCCAAATC GGCATCTCCA TGGAAGTCTG TTCGCACAGC TGTGCTCGCG
    GTGGCCACCT TCAACTCTAT CAAGGACTTG AACGAGCGCT GGAAATCACT TCAACAGCTG
    GCAGAAGAAA GGAGTCAGCT TCTTGGCAGT GCCCATGAAG TACAGAGATT CCACAGAGAT
    GCTGATGAGA CAAAAGAATG GATCGAAGAG AAGAACCAGG CTTTGAATAC AGACAGCTAT
    GGACATGACT TGGCCAGTGT GCAGGCTCTG CAACGAAAAC ATGAAGGCTT TGAGAGAGAC
    CTCGCCGCTC TGGGAGATAA GGTGAACTCT TTGGGAGAAA CTGCTGAGCG CCTGATCCAG
    TCACACCCTG AGTCTGCAGA TGACATTCAG GAAAAATGCA CAGAGCTGAA TCAGGCCTGG
    AACAGCCTTG GCACCCGGGC AAACCAGCGC AAGGAGAAAC TAGGCGATTC TCATGATCTG
    CAACGCTTCC TAAGTGACTT CAGAGATCTC ATGTCCTGGA TCAATGGAAT TCGGGGCTTG
    GTGTCATCAG ATGAACTTGC TAGAGATGTG ACTGGTGCAG AGGCTTTGCT GGAACGACAC
    CAGGAGCACA GGACTGAGAT TGATGCCAGA GCAGGCACTT TTCAGGCCTT TGAGCAGTTT
    GGGCAGCAAC TTCTGGCTCG TGGTCATTAT GATAGTCCTG AAATCAAGGA GAAGCTGGAT
    ATCTTGGAAC AGGAACGGGC AAGTTTGGAA AAGGCTTGGG TGGAACGCAG AATGATGTTG
    GATCAGTGCT TGGAGCTGCA GCTCTTCAAC AGAGACTGCG AGCAGGCAGA GAACTGGATG
    GCAGCCCGTG AAGCCTTCCT GAACAGTGAG GATAAAGGGG ATTCCCTGGA CAGTGTGGAG
    GCCCTTATCA AGAAACATGA AGACTTTGAT AAAGCCATTA ATGTCCAGGA AGAGAAGATT
    GCTGCCCTCC AGGCCTTCGC CGAACAATTG ATAATTGCTG ACCACTATGC CAAAGGAGAT
    ATTGCCTCCC GTCGCAATGA AGTCCTGGAC AGGTGGCGCC GTCTCAAGGC TCAGATGATT
    GAGAAGAGAT CCAAACTTGG GGAATCTCAG ACTCTGCAGC AGTTCAGCAG GGACGTCGAT
    GAGATCGAAG CCTGGATCAG CGAGAAATTG CAGACCGCCA GTGACGAGTC CTACAAAGAC
    CCCACAAACA TCCAGCTTTC CAAATTGCTG AGCAAACACC AGAAGCACCA GGCTTTCGAA
    GCTGAGCTCC ATGCCAACGC TGACCGTATT CGTGGAGTAG TAGATATGGG GAACTCCCTT
    ATCGAACGAG GTGCCTGTGC TGGCAGCGAG GATGCGGTGA AGGCTCGCCT AAGCGCACTC
    GCTGATCAAT GGCAGTTCCT TGTCAGCAAA TCCGCAGAGA AGAGCCAGAA ACTGAAGGAG
    GCAAACAAGC AGCAGAACTT TAACACTGGC ATCAAAGACT TTGATTTCTG GTTAACAGAG
    GTGGAGGCTT TGCTGGCATC TGAAGACTAT GGGAAGGACT TGGCATCTGT TAACAACCTG
    CTGAAGAAAC ACCAGCTTCT GGAAGCTGAC ATCTCTGCTC ATGAGGACCG CCTTAAAGAT
    CTTAATGGGC AGGCTGATAG CCTGATGACC AGCAGCGCCT TTGACACCAC CCAAGTAAAG
    GAGAAAAGGG ACGCCATCAA CGACCGCTTC CAGCGTATCA AAAGCATGGC CACCGCACGC
    CGCAGCAGGC TGAATGAGTC CCACCGTCTG CACCAGTTCT TCAGAGATCT TGATGATGAG
    GAATCATGGA TCAAGGAAAA GAAACTTTTG GTCGGATCTG ATGACTATGG ACGTGACTTG
    ACTGGAGTGC AAAATCTGAG GAAGAAACAC AAGAGATTAG AAGCTGAACT GGCAGCTCAT
    GAGCCAGCCA TTCAGGGTGT GCTGGACACT GGGAAGAAGC TTGCAGATGA CAACACTATT
    GGGAAAGATG AGATTCAGCA GCGACTGGCA CAGTTTCTAG AGCACTGGAA GGAGCTGAAG
    GAACAAGCAG CGGCTCGTGG TCAGAGACTG GAGGAATCTC TTGAGTACCA ACAATTTGTA
    GCCAATGTGG AGGAAGAGGA AGCCTGGATT AATGAGAAGA TGACTCTAGT GGCCAGTGAA
    GACTATGGTG ACACACTGGC TGCCATTCAG GGATTGCTGA AAAAGCACGA GGCATTTGAG
    ACAGACTTTA CAGTGCACAA AGACAGAGTA CATGATGTAT GTGCCAACGG GGAAGACCTG
    ATACAAAAGA ACAATCACCA TGTGGAGAAT ATTAGCGCCA AAATGAAGGG TCTCCGAGGG
    AAGGTGGCAG AACTAGAGAA GGCGGCTGCA CAGCGCAAGG CCAAACTTGA TGAGAATTCA
    GCTTTCCTGC AGTTCAACTG GAAGGCGGAT GTTGTGGAGT CCTGGATCGG TGAAAAAGAG
    AACAGCCTGA AGACTGATGA TTATGGCAGA GACCTGTCCT CTGTGCAGAC CCTTCTCACT
    AAGCAGGAAA CATTTGATGC TGGGCTGCAA GCTTTCCAGC AAGAGGGCAT TACCAACATC
    ACTGCACTGA AAGACCAGCT TCTGGCTGCA AAGCATGTGC AGTCCCGGGC CATAGAGTCT
    CGTCACGCTT CTCTGATGAA GCGGTGGAAC CAGTTGTTGG ATAACTCTGC TGCCCGCAAA
    AAGAAGTTAT TGGAGGCCCA GGAACACTAC AGAAAGGTTG AGGACTTGTT CCTAACATTT
    GCCAAGAAAG CCTCTGCCTT CAACAGCTGG TTTGAGAATG CCGAGGAGGA TTTGACTGAT
    CCTGTTCGCT GCAACTCCCT GGAGGAAATC AAGGCGCTTC GCGAGGCACA TGACGCCTTC
    CGCAATTCCC TCAGCTCCGC ACAGACAGAT TTCAACCAGC TGGCCGAACT GGACAGGCAA
    ATAAAGGGCT ACCGGGTAGC CTCCAATCCC TACACGTGGT TCACGATGGA GGCTTTGGAG
    GAGACATGGC GCAACCTGCA GAAAATCATT AAGGAACGAG AACTTGAGCT ACAAAAAGAA
    CAACGTAGGC AGGAAGAGAA CGATAAGCTC CGGCAAGAGT TTGCTCAGCA CGCCAATGCT
    TTCCACCAGT GGATTCAGGA GACAAGGACC TACCTTCTGG ACGGGTCCTG TATGGTGGAA
    GAATCTGGAA CACTGGAGTC ACAACTTGAA GCTACAAAGA GAAAGCATCA GGAGATCCGG
    GCCATGAGGA GTCAACTGAA GAAGATAGAA GATCTTGGCG CAGCCATGGA AGAGGCCCTG
    ATCTTGGATA ATAAATACAC AGAACACAGC ACTGTTGGGC TGGCACAGCA ATGGGACCAA
    CTGGATCAGC TAGGCATGAG AATGCAACAC AACCTAGAGC AACAGATCCA GGCCAGAAAC
    ACAACTGGGG TCACAGAGGA AGCACTCAAA GAATTCAGCA TGATGTTCAA GCATTTCGAC
    AAAGACAAGT CTGGCCGCCT CAACCACCAA GAATTCAAAT CTTGTCTGCG GTCCCTTGGC
    TATGATCTGC CCATGGTAGA AGAAGGAGAA CCAGACCCAG AGTTTGAAGC CATTTTGGAC
    ACTGTAGATC CCAACAGAGA TGGGCACGTT TCTCTACAGG AGTACATGGC TTTCATGATT
    AGCAGAGAAA CTGAAAACGT CAAGTCCAGT GAGGAGATAG AGAGCGCCTT CCGAGCACTG
    AGTACAGACC AGAAACCTTA TGTCACAAAG GAAGAACTAT ACCAGAACCT GACACGCGAA
    CAGGCAGATT ACTGCATCTC GCATATGAAA CCCTACATGG ACAGCAAGGG CCGCGAACTG
    CCCTCTGCAT ACGACTACGT GGAATTCACG CGCTCGCTAT TTGTGAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012823249.1
    CDS264..7634
    Translation

    Target ORF information:

    RefSeq Version XM_012967795.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis spectrin, alpha, non-erythrocytic 1 (sptan1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012967795.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGGATTCAG GTGGCGTGAA AGTGCTGGAG ACAGCGGAGG ACATCCAGGA AAGGCGCCAG 
    CAAGTCCTAG ACCGATACCT GCGGTTTAAG GAACTCTCTG TACTGAGACG TCAGAAGTTG
    GAGGATTCTT ACCGCTTCCA GTTCTTCCAG CGAGATGCTG ATGAACTGGA GAAATGGATC
    CAGGAGAAAC TCCAGATCGC ATCTGATGAG AACTACAAAG ATCCAACCAA TCTGCAGGGC
    AAATTGCAGA AACACCAGGC TTTTGAGGCA GAAGTACAGG CCAATGCAGG GGCAATTGTC
    AAACTGGACG AAACTGGAAA CCTGATGATT TCAGAAGGAC ATTTTTCATC AGAGACCATC
    AGAACCCGCC TGGTAGAGTT GCATCGTCTG TGGGAGCTGC TGCTCTTAAA GATGCAAGAG
    AAAGGGGTTA AATTACAACA GGCGCAGAAG CTTGTTCAGT ATCTACGGGA ATGTGAAGAT
    GTCCTAGACT GGATTAATGA CAAGGAGGCT ATTGTAACAT CTGAGGAGCT TGGTCAGGAT
    TTGGAACATG TGGAGGTCTT ACAGAAGAAG TTTGAAGAGT TTCAGACTGA TCTGGCAGCT
    CATGAAGAAA GAGTGAATGA AGTCAACCAG TTTGCAAGTA AACTGGCCCA GGAGGAACAC
    CCGGAGCTGG AGCTAATAAA GACCAAGCAG GATGAAGTCA ATGCAAGTTG GCAGCGCCTC
    AAAGGGCTGG CACTTCAGAG ACAAGGGAAG TTATTTGGAG CTGCAGAAGT TCAGCGTTTT
    AACAGAGATG TTGATGAAAC CATTGGATGG ATAAGAGAGA AGGAGCAGCT GATGGCATCT
    GATGACTTTG GCCGAGACCT GGCTAGCGTT CAAGCTCTCC TACGTAAACA CGAGGGTCTG
    GAGAGAGACC TTGCAGCACT GGAGGAAAAG GTAAAGGCCT TGTGTGCAGA ATCAGACCGT
    CTGCAGCAAT CCCATCCCAT GAACGCCCCT CAAATTCAAG TGAAAGGAGA AGAACTGATA
    ACCAACTGGG AGCAAATACG CACATTGGCA GCAGAACGAC ATGCTCGACT CAATGACTCT
    TACAAGCTTC AGCGTTTCCT TGCAGATTTT AGAGATCTGA CTAGCTGGGT GACTGAAATG
    AAAGCTCTGA TTAATGCCGA TGAGCTTGCC AACGATGTGG CTGGCGCAGA GGCTCTGCTT
    GACCGACACC AGGAACATAA GGGGGAAATT GATGCTCATG AAGATAGCTT TAAAGCTGCA
    GATGCTGCAG GTCAGGCCCT TCTGAACGGT GGCCACTACG CCTCAGATGA AGTCCGAGAA
    AAGCTTACTA TCTTAGCAGA AGAAAGGGCA GCTCTTCTAG AGCTTTGGGA GCTCCGTCGT
    CAGCAGTATG AACAGTGCAT GGACCTGCAG CTTTTTTACA GAGACACCGA ACAGGTGGAC
    AACTGGATGA GCAAACAGGA AGCTTTCCTG CTTAACGAGG ACTTGGGTGA TTCTCTAGAC
    AGTGTGGAAG CTCTTCTGAA AAAACATGAG GACTTTGAGA AGTCTCTTAG CGCCCAGGAA
    GAGAAAATCA CAGCTCTGGA TGAGTTTGCT ACAAAACTAA TTCAAAATAA CCACTATGCC
    AAGGAAGATG TGGCTACCCG CAGAGATGCT CTGCTGAGCC GCCGCAATGC ACTTCATGAA
    CGTGCACTCT ACCGCCGTAC TCAACTGGCA GACTCCTTTC ACCTGCAGCA GTTCTTTAGG
    GACTCTGACG AGCTGAAAAG TTGGATTAAT GAAAAGATGA AGACTGCAAC GGATGAGGCA
    TACAAGGATC CCTCCAACCT CCAGGGTAAG GTGCAGAAAC ACCAAGCTTT TGAGGCTGAA
    CTCTCCGCTA ACCAAAGCCG TATAGATGCC CTGGAGAATT CTGGGCAGAA ATTGATTGAT
    GTCAACCATT ATGCATCAGA TGAGGTGGCA GCACGTATGA GTGAGGTCAT CACTTTGTGG
    AAGAAGCTGC TGGAGGCCAC TGAATTGAAG GGGATCAAGC TGCGCGAAGC CAACCAGCAG
    CAGCAGTTCA ACCGTAATGT GGAGGATATA GAGCTGTGGC TGTATGAGGT TGAGGGGCAT
    TTGGCTTCAG ATGACTATGG AAAAGACCTG ACCAATGTTC AAAACTTGCA GAAAAAACAC
    GCCTTGCTGG AGGCTGATGT TGCAGCTCAC CAGGACCGTA TCGATGGCAT CACTATCCAA
    GCCCGTCAGT TCCAAGAAGC TGGTCATTTT GATGCTGACA ATATCAGGAA GAAACAGGAG
    GCCCTTGTTA ATAGATATGA AGCTTTGAAA GAACCCATGG TTGCACGCAA GCAGAAGCTG
    TCAGACTCTC TGCGTCTTCA ACAGCTTTTC AGGGATGTTG AAGATGAGGA AACCTGGATA
    AGGGAGAAGG AACCCATTGC AGCGTCCACC AACAGAGGCA AGGATTTGAT TGGAGTCCAG
    AATCTCCTGA AGAAACACCA GGCTTTGCAG GCTGAAATTG CAGGGCACGA ACCACGAATT
    AAAGCTGTTA CCCAGAAAGG AGAATCTATG ATCTCTGAAG GCCACTTTGC TTCTGATGAA
    GTGAAGGGCA AATTAAGGGA GCTGAATGAT AAGTGGGCCA CCCTGAAGAA CAAAGCATCT
    CAGCGCCGAC AGGACCTTGA AGATTCTCTG CAAGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCAGAGT CCTGGATGAG AGAGAAGGAA CCTATTGTTG GAAGCACCGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC CCTTCTGAAG AAACATGAAG CGCTTATGTC AGATCTGCGA
    GCATACGGAA GCAGTATTCA GGGACTTAGA GAGCAGGCCC AGGCCTGCAG GCAACAAGTT
    GCTCCAACTG ATGATGAAAC TGGAAAGGAG CTGGTGCTGG CATTGTATGA TTATCAAGAG
    AAGAGCCCTA GGGAGGTGAC AATGAAGAAA GGAGACATCT TGACCTTGCT AAACAGCACA
    AATAAGGATT GGTGGAAGGT GGAAGTGAAT GATCGTCAGG GCTTTGTACC TGCTGCGTAT
    GTGAAAAAGC TGGACCCAGC ACAGTCCGCA TCCCGGGAAA ACTTGCTGGA GGAACAGGGA
    AGCATCGCTC TCCGTCAGGA GCAGATCGAT GGCCAGTATC ATAATATTTT GGAGATGGGA
    GAGAAGCGCA AAGATATGTT AGAAAAGAGC TGCAAGAAGT TTATGCTGTT CCGTGAGGCA
    AATGAGCTGC AGCAGTGGAT TAACGAGAAA GAAGCATCAC TCACTAACGA AGAGGTCGGG
    GCTGACCTGG AACAAGTGGA AGTGCTGCAG AAGAAGTTTG ATGATTTCCA GAAGGATCTG
    AAAGCAAATG AGTCACGCTT GAAGGACATC AACAAGGTGG CCGACGAGCT TGAATCTGAA
    GGCCTCATTA CAGAGGAAGT CCAGCCGGTG CAGCATCAGG AGGTGTATGG GATGCAGCGG
    GATGAAGCAG ACTCCAAATC GGCATCTCCA TGGAAGTCTG TTCGCACAGC TGTGCTCGCG
    GTGGCCACCT TCAACTCTAT CAAGGACTTG AACGAGCGCT GGAAATCACT TCAACAGCTG
    GCAGAAGAAA GGAGTCAGCT TCTTGGCAGT GCCCATGAAG TACAGAGATT CCACAGAGAT
    GCTGATGAGA CAAAAGAATG GATCGAAGAG AAGAACCAGG CTTTGAATAC AGACAGCTAT
    GGACATGACT TGGCCAGTGT GCAGGCTCTG CAACGAAAAC ATGAAGGCTT TGAGAGAGAC
    CTCGCCGCTC TGGGAGATAA GGTGAACTCT TTGGGAGAAA CTGCTGAGCG CCTGATCCAG
    TCACACCCTG AGTCTGCAGA TGACATTCAG GAAAAATGCA CAGAGCTGAA TCAGGCCTGG
    AACAGCCTTG GCACCCGGGC AAACCAGCGC AAGGAGAAAC TAGGCGATTC TCATGATCTG
    CAACGCTTCC TAAGTGACTT CAGAGATCTC ATGTCCTGGA TCAATGGAAT TCGGGGCTTG
    GTGTCATCAG ATGAACTTGC TAGAGATGTG ACTGGTGCAG AGGCTTTGCT GGAACGACAC
    CAGGAGCACA GGACTGAGAT TGATGCCAGA GCAGGCACTT TTCAGGCCTT TGAGCAGTTT
    GGGCAGCAAC TTCTGGCTCG TGGTCATTAT GATAGTCCTG AAATCAAGGA GAAGCTGGAT
    ATCTTGGAAC AGGAACGGGC AAGTTTGGAA AAGGCTTGGG TGGAACGCAG AATGATGTTG
    GATCAGTGCT TGGAGCTGCA GCTCTTCAAC AGAGACTGCG AGCAGGCAGA GAACTGGATG
    GCAGCCCGTG AAGCCTTCCT GAACAGTGAG GATAAAGGGG ATTCCCTGGA CAGTGTGGAG
    GCCCTTATCA AGAAACATGA AGACTTTGAT AAAGCCATTA ATGTCCAGGA AGAGAAGATT
    GCTGCCCTCC AGGCCTTCGC CGAACAATTG ATAATTGCTG ACCACTATGC CAAAGGAGAT
    ATTGCCTCCC GTCGCAATGA AGTCCTGGAC AGGTGGCGCC GTCTCAAGGC TCAGATGATT
    GAGAAGAGAT CCAAACTTGG GGAATCTCAG ACTCTGCAGC AGTTCAGCAG GGACGTCGAT
    GAGATCGAAG CCTGGATCAG CGAGAAATTG CAGACCGCCA GTGACGAGTC CTACAAAGAC
    CCCACAAACA TCCAGCTTTC CAAATTGCTG AGCAAACACC AGAAGCACCA GGCTTTCGAA
    GCTGAGCTCC ATGCCAACGC TGACCGTATT CGTGGAGTAG TAGATATGGG GAACTCCCTT
    ATCGAACGAG GTGCCTGTGC TGGCAGCGAG GATGCGGTGA AGGCTCGCCT AAGCGCACTC
    GCTGATCAAT GGCAGTTCCT TGTCAGCAAA TCCGCAGAGA AGAGCCAGAA ACTGAAGGAG
    GCAAACAAGC AGCAGAACTT TAACACTGGC ATCAAAGACT TTGATTTCTG GTTAACAGAG
    GTGGAGGCTT TGCTGGCATC TGAAGACTAT GGGAAGGACT TGGCATCTGT TAACAACCTG
    CTGAAGAAAC ACCAGCTTCT GGAAGCTGAC ATCTCTGCTC ATGAGGACCG CCTTAAAGAT
    CTTAATGGGC AGGCTGATAG CCTGATGACC AGCAGCGCCT TTGACACCAC CCAAGTAAAG
    GAGAAAAGGG ACGCCATCAA CGACCGCTTC CAGCGTATCA AAAGCATGGC CACCGCACGC
    CGCAGCAGGC TGAATGAGTC CCACCGTCTG CACCAGTTCT TCAGAGATCT TGATGATGAG
    GAATCATGGA TCAAGGAAAA GAAACTTTTG GTCGGATCTG ATGACTATGG ACGTGACTTG
    ACTGGAGTGC AAAATCTGAG GAAGAAACAC AAGAGATTAG AAGCTGAACT GGCAGCTCAT
    GAGCCAGCCA TTCAGGGTGT GCTGGACACT GGGAAGAAGC TTGCAGATGA CAACACTATT
    GGGAAAGATG AGATTCAGCA GCGACTGGCA CAGTTTCTAG AGCACTGGAA GGAGCTGAAG
    GAACAAGCAG CGGCTCGTGG TCAGAGACTG GAGGAATCTC TTGAGTACCA ACAATTTGTA
    GCCAATGTGG AGGAAGAGGA AGCCTGGATT AATGAGAAGA TGACTCTAGT GGCCAGTGAA
    GACTATGGTG ACACACTGGC TGCCATTCAG GGATTGCTGA AAAAGCACGA GGCATTTGAG
    ACAGACTTTA CAGTGCACAA AGACAGAGTA CATGATGTAT GTGCCAACGG GGAAGACCTG
    ATACAAAAGA ACAATCACCA TGTGGAGAAT ATTAGCGCCA AAATGAAGGG TCTCCGAGGG
    AAGGTGGCAG AACTAGAGAA GGCGGCTGCA CAGCGCAAGG CCAAACTTGA TGAGAATTCA
    GCTTTCCTGC AGTTCAACTG GAAGGCGGAT GTTGTGGAGT CCTGGATCGG TGAAAAAGAG
    AACAGCCTGA AGACTGATGA TTATGGCAGA GACCTGTCCT CTGTGCAGAC CCTTCTCACT
    AAGCAGGAAA CATTTGATGC TGGGCTGCAA GCTTTCCAGC AAGAGGGCAT TACCAACATC
    ACTGCACTGA AAGACCAGCT TCTGGCTGCA AAGCATGTGC AGTCCCGGGC CATAGAGTCT
    CGTCACGCTT CTCTGATGAA GCGGTGGAAC CAGTTGTTGG ATAACTCTGC TGCCCGCAAA
    AAGAAGTTAT TGGAGGCCCA GGAACACTAC AGAAAGGTTG AGGACTTGTT CCTAACATTT
    GCCAAGAAAG CCTCTGCCTT CAACAGCTGG TTTGAGAATG CCGAGGAGGA TTTGACTGAT
    CCTGTTCGCT GCAACTCCCT GGAGGAAATC AAGGCGCTTC GCGAGGCACA TGACGCCTTC
    CGCAATTCCC TCAGCTCCGC ACAGACAGAT TTCAACCAGC TGGCCGAACT GGACAGGCAA
    ATAAAGGGCT ACCGGGTAGC CTCCAATCCC TACACGTGGT TCACGATGGA GGCTTTGGAG
    GAGACATGGC GCAACCTGCA GAAAATCATT AAGGAACGAG AACTTGAGCT ACAAAAAGAA
    CAACGTAGGC AGGAAGAGAA CGATAAGCTC CGGCAAGAGT TTGCTCAGCA CGCCAATGCT
    TTCCACCAGT GGATTCAGGA GACAAGGACC TACCTTCTGG ACGGGTCCTG TATGGTGGAA
    GAATCTGGAA CACTGGAGTC ACAACTTGAA GCTACAAAGA GAAAGCATCA GGAGATCCGG
    GCCATGAGGA GTCAACTGAA GAAGATAGAA GATCTTGGCG CAGCCATGGA AGAGGCCCTG
    ATCTTGGATA ATAAATACAC AGAACACAGC ACTGTTGGGC TGGCACAGCA ATGGGACCAA
    CTGGATCAGC TAGGCATGAG AATGCAACAC AACCTAGAGC AACAGATCCA GGCCAGAAAC
    ACAACTGGGG TCACAGAGGA AGCACTCAAA GAATTCAGCA TGATGTTCAA GCATTTCGAC
    AAAGACAAGT CTGGCCGCCT CAACCACCAA GAATTCAAAT CTTGTCTGCG GTCCCTTGGC
    TATGATCTGC CCATGGTAGA AGAAGGAGAA CCAGACCCAG AGTTTGAAGC CATTTTGGAC
    ACTGTAGATC CCAACAGAGA TGGGCACGTT TCTCTACAGG AGTACATGGC TTTCATGATT
    AGCAGAGAAA CTGAAAACGT CAAGTCCAGT GAGGAGATAG AGAGCGCCTT CCGAGCACTG
    AGTACAGACC AGAAACCTTA TGTCACAAAG GAAGAACTAT ACCAGAACCT GACACGCGAA
    CAGGCAGATT ACTGCATCTC GCATATGAAA CCCTACATGG ACAGCAAGGG CCGCGAACTG
    CCCTCTGCAT ACGACTACGT GGAATTCACG CGCTCGCTAT TTGTGAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29837
    Clone ID Related Accession (Same CDS sequence) XM_012967796.2 , XM_031906808.1
    Accession Version XM_012967796.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7356bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012967796.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGGATTCAG GTGGCGTGAA AGTGCTGGAG ACAGCGGAGG ACATCCAGGA AAGGCGCCAG 
    CAAGTCCTAG ACCGATACCT GCGGTTTAAG GAACTCTCTG TACTGAGACG TCAGAAGTTG
    GAGGATTCTT ACCGCTTCCA GTTCTTCCAG CGAGATGCTG ATGAACTGGA GAAATGGATC
    CAGGAGAAAC TCCAGATCGC ATCTGATGAG AACTACAAAG ATCCAACCAA TCTGCAGGGC
    AAATTGCAGA AACACCAGGC TTTTGAGGCA GAAGTACAGG CCAATGCAGG GGCAATTGTC
    AAACTGGACG AAACTGGAAA CCTGATGATT TCAGAAGGAC ATTTTTCATC AGAGACCATC
    AGAACCCGCC TGGTAGAGTT GCATCGTCTG TGGGAGCTGC TGCTCTTAAA GATGCAAGAG
    AAAGGGGTTA AATTACAACA GGCGCAGAAG CTTGTTCAGT ATCTACGGGA ATGTGAAGAT
    GTCCTAGACT GGATTAATGA CAAGGAGGCT ATTGTAACAT CTGAGGAGCT TGGTCAGGAT
    TTGGAACATG TGGAGGTCTT ACAGAAGAAG TTTGAAGAGT TTCAGACTGA TCTGGCAGCT
    CATGAAGAAA GAGTGAATGA AGTCAACCAG TTTGCAAGTA AACTGGCCCA GGAGGAACAC
    CCGGAGCTGG AGCTAATAAA GACCAAGCAG GATGAAGTCA ATGCAAGTTG GCAGCGCCTC
    AAAGGGCTGG CACTTCAGAG ACAAGGGAAG TTATTTGGAG CTGCAGAAGT TCAGCGTTTT
    AACAGAGATG TTGATGAAAC CATTGGATGG ATAAGAGAGA AGGAGCAGCT GATGGCATCT
    GATGACTTTG GCCGAGACCT GGCTAGCGTT CAAGCTCTCC TACGTAAACA CGAGGGTCTG
    GAGAGAGACC TTGCAGCACT GGAGGAAAAG GTAAAGGCCT TGTGTGCAGA ATCAGACCGT
    CTGCAGCAAT CCCATCCCAT GAACGCCCCT CAAATTCAAG TGAAAGGAGA AGAACTGATA
    ACCAACTGGG AGCAAATACG CACATTGGCA GCAGAACGAC ATGCTCGACT CAATGACTCT
    TACAAGCTTC AGCGTTTCCT TGCAGATTTT AGAGATCTGA CTAGCTGGGT GACTGAAATG
    AAAGCTCTGA TTAATGCCGA TGAGCTTGCC AACGATGTGG CTGGCGCAGA GGCTCTGCTT
    GACCGACACC AGGAACATAA GGGGGAAATT GATGCTCATG AAGATAGCTT TAAAGCTGCA
    GATGCTGCAG GTCAGGCCCT TCTGAACGGT GGCCACTACG CCTCAGATGA AGTCCGAGAA
    AAGCTTACTA TCTTAGCAGA AGAAAGGGCA GCTCTTCTAG AGCTTTGGGA GCTCCGTCGT
    CAGCAGTATG AACAGTGCAT GGACCTGCAG CTTTTTTACA GAGACACCGA ACAGGTGGAC
    AACTGGATGA GCAAACAGGA AGCTTTCCTG CTTAACGAGG ACTTGGGTGA TTCTCTAGAC
    AGTGTGGAAG CTCTTCTGAA AAAACATGAG GACTTTGAGA AGTCTCTTAG CGCCCAGGAA
    GAGAAAATCA CAGCTCTGGA TGAGTTTGCT ACAAAACTAA TTCAAAATAA CCACTATGCC
    AAGGAAGATG TGGCTACCCG CAGAGATGCT CTGCTGAGCC GCCGCAATGC ACTTCATGAA
    CGTGCACTCT ACCGCCGTAC TCAACTGGCA GACTCCTTTC ACCTGCAGCA GTTCTTTAGG
    GACTCTGACG AGCTGAAAAG TTGGATTAAT GAAAAGATGA AGACTGCAAC GGATGAGGCA
    TACAAGGATC CCTCCAACCT CCAGGGTAAG GTGCAGAAAC ACCAAGCTTT TGAGGCTGAA
    CTCTCCGCTA ACCAAAGCCG TATAGATGCC CTGGAGAATT CTGGGCAGAA ATTGATTGAT
    GTCAACCATT ATGCATCAGA TGAGGTGGCA GCACGTATGA GTGAGGTCAT CACTTTGTGG
    AAGAAGCTGC TGGAGGCCAC TGAATTGAAG GGGATCAAGC TGCGCGAAGC CAACCAGCAG
    CAGCAGTTCA ACCGTAATGT GGAGGATATA GAGCTGTGGC TGTATGAGGT TGAGGGGCAT
    TTGGCTTCAG ATGACTATGG AAAAGACCTG ACCAATGTTC AAAACTTGCA GAAAAAACAC
    GCCTTGCTGG AGGCTGATGT TGCAGCTCAC CAGGACCGTA TCGATGGCAT CACTATCCAA
    GCCCGTCAGT TCCAAGAAGC TGGTCATTTT GATGCTGACA ATATCAGGAA GAAACAGGAG
    GCCCTTGTTA ATAGATATGA AGCTTTGAAA GAACCCATGG TTGCACGCAA GCAGAAGCTG
    TCAGACTCTC TGCGTCTTCA ACAGCTTTTC AGGGATGTTG AAGATGAGGA AACCTGGATA
    AGGGAGAAGG AACCCATTGC AGCGTCCACC AACAGAGGCA AGGATTTGAT TGGAGTCCAG
    AATCTCCTGA AGAAACACCA GGCTTTGCAG GCTGAAATTG CAGGGCACGA ACCACGAATT
    AAAGCTGTTA CCCAGAAAGG AGAATCTATG ATCTCTGAAG GCCACTTTGC TTCTGATGAA
    GTGAAGGGCA AATTAAGGGA GCTGAATGAT AAGTGGGCCA CCCTGAAGAA CAAAGCATCT
    CAGCGCCGAC AGGACCTTGA AGATTCTCTG CAAGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCAGAGT CCTGGATGAG AGAGAAGGAA CCTATTGTTG GAAGCACCGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC CCTTCTGAAG AAACATGAAG CGCTTATGTC AGATCTGCGA
    GCATACGGAA GCAGTATTCA GGGACTTAGA GAGCAGGCCC AGGCCTGCAG GCAACAAGTT
    GCTCCAACTG ATGATGAAAC TGGAAAGGAG CTGGTGCTGG CATTGTATGA TTATCAAGAG
    AAGAGCCCTA GGGAGGTGAC AATGAAGAAA GGAGACATCT TGACCTTGCT AAACAGCACA
    AATAAGGATT GGTGGAAGGT GGAAGTGAAT GATCGTCAGG GCTTTGTACC TGCTGCGTAT
    GTGAAAAAGC TGGACCCAGC ACAGTCCGCA TCCCGGGAAA ACTTGCTGGA GGAACAGGGA
    AGCATCGCTC TCCGTCAGGA GCAGATCGAT GGCCAGTATC ATAATATTTT GGAGATGGGA
    GAGAAGCGCA AAGATATGTT AGAAAAGAGC TGCAAGAAGT TTATGCTGTT CCGTGAGGCA
    AATGAGCTGC AGCAGTGGAT TAACGAGAAA GAAGCATCAC TCACTAACGA AGAGGTCGGG
    GCTGACCTGG AACAAGTGGA AGTGCTGCAG AAGAAGTTTG ATGATTTCCA GAAGGATCTG
    AAAGCAAATG AGTCACGCTT GAAGGACATC AACAAGGTGG CCGACGAGCT TGAATCTGAA
    GGCCTCATTA CAGAGGAAGT CCAGCCGGTG CAGCATCAGG AGGTGTATGG GATGCAGCGG
    GATGAAGCAG ACTCCAAATC GGCATCTCCA TGGAAGTCTG TTCGCACAGC TGTGCTCGCG
    GTGGCCACCT TCAACTCTAT CAAGGACTTG AACGAGCGCT GGAAATCACT TCAACAGCTG
    GCAGAAGAAA GGAGTCAGCT TCTTGGCAGT GCCCATGAAG TACAGAGATT CCACAGAGAT
    GCTGATGAGA CAAAAGAATG GATCGAAGAG AAGAACCAGG CTTTGAATAC AGACAGCTAT
    GGACATGACT TGGCCAGTGT GCAGGCTCTG CAACGAAAAC ATGAAGGCTT TGAGAGAGAC
    CTCGCCGCTC TGGGAGATAA GGTGAACTCT TTGGGAGAAA CTGCTGAGCG CCTGATCCAG
    TCACACCCTG AGTCTGCAGA TGACATTCAG GAAAAATGCA CAGAGCTGAA TCAGGCCTGG
    AACAGCCTTG GCACCCGGGC AAACCAGCGC AAGGAGAAAC TAGGCGATTC TCATGATCTG
    CAACGCTTCC TAAGTGACTT CAGAGATCTC ATGTCCTGGA TCAATGGAAT TCGGGGCTTG
    GTGTCATCAG ATGAACTTGC TAGAGATGTG ACTGGTGCAG AGGCTTTGCT GGAACGACAC
    CAGGAGCACA GGACTGAGAT TGATGCCAGA GCAGGCACTT TTCAGGCCTT TGAGCAGTTT
    GGGCAGCAAC TTCTGGCTCG TGGTCATTAT GATAGTCCTG AAATCAAGGA GAAGCTGGAT
    ATCTTGGAAC AGGAACGGGC AAGTTTGGAA AAGGCTTGGG TGGAACGCAG AATGATGTTG
    GATCAGTGCT TGGAGCTGCA GCTCTTCAAC AGAGACTGCG AGCAGGCAGA GAACTGGATG
    GCAGCCCGTG AAGCCTTCCT GAACAGTGAG GATAAAGGGG ATTCCCTGGA CAGTGTGGAG
    GCCCTTATCA AGAAACATGA AGACTTTGAT AAAGCCATTA ATGTCCAGGA AGAGAAGATT
    GCTGCCCTCC AGGCCTTCGC CGAACAATTG ATAATTGCTG ACCACTATGC CAAAGGAGAT
    ATTGCCTCCC GTCGCAATGA AGTCCTGGAC AGGTGGCGCC GTCTCAAGGC TCAGATGATT
    GAGAAGAGAT CCAAACTTGG GGAATCTCAG ACTCTGCAGC AGTTCAGCAG GGACGTCGAT
    GAGATCGAAG CCTGGATCAG CGAGAAATTG CAGACCGCCA GTGACGAGTC CTACAAAGAC
    CCCACAAACA TCCAGAGCAA ACACCAGAAG CACCAGGCTT TCGAAGCTGA GCTCCATGCC
    AACGCTGACC GTATTCGTGG AGTAGTAGAT ATGGGGAACT CCCTTATCGA ACGAGGTGCC
    TGTGCTGGCA GCGAGGATGC GGTGAAGGCT CGCCTAAGCG CACTCGCTGA TCAATGGCAG
    TTCCTTGTCA GCAAATCCGC AGAGAAGAGC CAGAAACTGA AGGAGGCAAA CAAGCAGCAG
    AACTTTAACA CTGGCATCAA AGACTTTGAT TTCTGGTTAA CAGAGGTGGA GGCTTTGCTG
    GCATCTGAAG ACTATGGGAA GGACTTGGCA TCTGTTAACA ACCTGCTGAA GAAACACCAG
    CTTCTGGAAG CTGACATCTC TGCTCATGAG GACCGCCTTA AAGATCTTAA TGGGCAGGCT
    GATAGCCTGA TGACCAGCAG CGCCTTTGAC ACCACCCAAG TAAAGGAGAA AAGGGACGCC
    ATCAACGACC GCTTCCAGCG TATCAAAAGC ATGGCCACCG CACGCCGCAG CAGGCTGAAT
    GAGTCCCACC GTCTGCACCA GTTCTTCAGA GATCTTGATG ATGAGGAATC ATGGATCAAG
    GAAAAGAAAC TTTTGGTCGG ATCTGATGAC TATGGACGTG ACTTGACTGG AGTGCAAAAT
    CTGAGGAAGA AACACAAGAG ATTAGAAGCT GAACTGGCAG CTCATGAGCC AGCCATTCAG
    GGTGTGCTGG ACACTGGGAA GAAGCTTGCA GATGACAACA CTATTGGGAA AGATGAGATT
    CAGCAGCGAC TGGCACAGTT TCTAGAGCAC TGGAAGGAGC TGAAGGAACA AGCAGCGGCT
    CGTGGTCAGA GACTGGAGGA ATCTCTTGAG TACCAACAAT TTGTAGCCAA TGTGGAGGAA
    GAGGAAGCCT GGATTAATGA GAAGATGACT CTAGTGGCCA GTGAAGACTA TGGTGACACA
    CTGGCTGCCA TTCAGGGATT GCTGAAAAAG CACGAGGCAT TTGAGACAGA CTTTACAGTG
    CACAAAGACA GAGTACATGA TGTATGTGCC AACGGGGAAG ACCTGATACA AAAGAACAAT
    CACCATGTGG AGAATATTAG CGCCAAAATG AAGGGTCTCC GAGGGAAGGT GGCAGAACTA
    GAGAAGGCGG CTGCACAGCG CAAGGCCAAA CTTGATGAGA ATTCAGCTTT CCTGCAGTTC
    AACTGGAAGG CGGATGTTGT GGAGTCCTGG ATCGGTGAAA AAGAGAACAG CCTGAAGACT
    GATGATTATG GCAGAGACCT GTCCTCTGTG CAGACCCTTC TCACTAAGCA GGAAACATTT
    GATGCTGGGC TGCAAGCTTT CCAGCAAGAG GGCATTACCA ACATCACTGC ACTGAAAGAC
    CAGCTTCTGG CTGCAAAGCA TGTGCAGTCC CGGGCCATAG AGTCTCGTCA CGCTTCTCTG
    ATGAAGCGGT GGAACCAGTT GTTGGATAAC TCTGCTGCCC GCAAAAAGAA GTTATTGGAG
    GCCCAGGAAC ACTACAGAAA GGTTGAGGAC TTGTTCCTAA CATTTGCCAA GAAAGCCTCT
    GCCTTCAACA GCTGGTTTGA GAATGCCGAG GAGGATTTGA CTGATCCTGT TCGCTGCAAC
    TCCCTGGAGG AAATCAAGGC GCTTCGCGAG GCACATGACG CCTTCCGCAA TTCCCTCAGC
    TCCGCACAGA CAGATTTCAA CCAGCTGGCC GAACTGGACA GGCAAATAAA GGGCTACCGG
    GTAGCCTCCA ATCCCTACAC GTGGTTCACG ATGGAGGCTT TGGAGGAGAC ATGGCGCAAC
    CTGCAGAAAA TCATTAAGGA ACGAGAACTT GAGCTACAAA AAGAACAACG TAGGCAGGAA
    GAGAACGATA AGCTCCGGCA AGAGTTTGCT CAGCACGCCA ATGCTTTCCA CCAGTGGATT
    CAGGAGACAA GGACCTACCT TCTGGACGGG TCCTGTATGG TGGAAGAATC TGGAACACTG
    GAGTCACAAC TTGAAGCTAC AAAGAGAAAG CATCAGGAGA TCCGGGCCAT GAGGAGTCAA
    CTGAAGAAGA TAGAAGATCT TGGCGCAGCC ATGGAAGAGG CCCTGATCTT GGATAATAAA
    TACACAGAAC ACAGCACTGT TGGGCTGGCA CAGCAATGGG ACCAACTGGA TCAGCTAGGC
    ATGAGAATGC AACACAACCT AGAGCAACAG ATCCAGGCCA GAAACACAAC TGGGGTCACA
    GAGGAAGCAC TCAAAGAATT CAGCATGATG TTCAAGCATT TCGACAAAGA CAAGTCTGGC
    CGCCTCAACC ACCAAGAATT CAAATCTTGT CTGCGGTCCC TTGGCTATGA TCTGCCCATG
    GTAGAAGAAG GAGAACCAGA CCCAGAGTTT GAAGCCATTT TGGACACTGT AGATCCCAAC
    AGAGATGGGC ACGTTTCTCT ACAGGAGTAC ATGGCTTTCA TGATTAGCAG AGAAACTGAA
    AACGTCAAGT CCAGTGAGGA GATAGAGAGC GCCTTCCGAG CACTGAGTAC AGACCAGAAA
    CCTTATGTCA CAAAGGAAGA ACTATACCAG AACCTGACAC GCGAACAGGC AGATTACTGC
    ATCTCGCATA TGAAACCCTA CATGGACAGC AAGGGCCGCG AACTGCCCTC TGCATACGAC
    TACGTGGAAT TCACGCGCTC GCTATTTGTG AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012823250.1
    CDS264..7619
    Translation

    Target ORF information:

    RefSeq Version XM_012967796.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis spectrin, alpha, non-erythrocytic 1 (sptan1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012967796.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGGATTCAG GTGGCGTGAA AGTGCTGGAG ACAGCGGAGG ACATCCAGGA AAGGCGCCAG 
    CAAGTCCTAG ACCGATACCT GCGGTTTAAG GAACTCTCTG TACTGAGACG TCAGAAGTTG
    GAGGATTCTT ACCGCTTCCA GTTCTTCCAG CGAGATGCTG ATGAACTGGA GAAATGGATC
    CAGGAGAAAC TCCAGATCGC ATCTGATGAG AACTACAAAG ATCCAACCAA TCTGCAGGGC
    AAATTGCAGA AACACCAGGC TTTTGAGGCA GAAGTACAGG CCAATGCAGG GGCAATTGTC
    AAACTGGACG AAACTGGAAA CCTGATGATT TCAGAAGGAC ATTTTTCATC AGAGACCATC
    AGAACCCGCC TGGTAGAGTT GCATCGTCTG TGGGAGCTGC TGCTCTTAAA GATGCAAGAG
    AAAGGGGTTA AATTACAACA GGCGCAGAAG CTTGTTCAGT ATCTACGGGA ATGTGAAGAT
    GTCCTAGACT GGATTAATGA CAAGGAGGCT ATTGTAACAT CTGAGGAGCT TGGTCAGGAT
    TTGGAACATG TGGAGGTCTT ACAGAAGAAG TTTGAAGAGT TTCAGACTGA TCTGGCAGCT
    CATGAAGAAA GAGTGAATGA AGTCAACCAG TTTGCAAGTA AACTGGCCCA GGAGGAACAC
    CCGGAGCTGG AGCTAATAAA GACCAAGCAG GATGAAGTCA ATGCAAGTTG GCAGCGCCTC
    AAAGGGCTGG CACTTCAGAG ACAAGGGAAG TTATTTGGAG CTGCAGAAGT TCAGCGTTTT
    AACAGAGATG TTGATGAAAC CATTGGATGG ATAAGAGAGA AGGAGCAGCT GATGGCATCT
    GATGACTTTG GCCGAGACCT GGCTAGCGTT CAAGCTCTCC TACGTAAACA CGAGGGTCTG
    GAGAGAGACC TTGCAGCACT GGAGGAAAAG GTAAAGGCCT TGTGTGCAGA ATCAGACCGT
    CTGCAGCAAT CCCATCCCAT GAACGCCCCT CAAATTCAAG TGAAAGGAGA AGAACTGATA
    ACCAACTGGG AGCAAATACG CACATTGGCA GCAGAACGAC ATGCTCGACT CAATGACTCT
    TACAAGCTTC AGCGTTTCCT TGCAGATTTT AGAGATCTGA CTAGCTGGGT GACTGAAATG
    AAAGCTCTGA TTAATGCCGA TGAGCTTGCC AACGATGTGG CTGGCGCAGA GGCTCTGCTT
    GACCGACACC AGGAACATAA GGGGGAAATT GATGCTCATG AAGATAGCTT TAAAGCTGCA
    GATGCTGCAG GTCAGGCCCT TCTGAACGGT GGCCACTACG CCTCAGATGA AGTCCGAGAA
    AAGCTTACTA TCTTAGCAGA AGAAAGGGCA GCTCTTCTAG AGCTTTGGGA GCTCCGTCGT
    CAGCAGTATG AACAGTGCAT GGACCTGCAG CTTTTTTACA GAGACACCGA ACAGGTGGAC
    AACTGGATGA GCAAACAGGA AGCTTTCCTG CTTAACGAGG ACTTGGGTGA TTCTCTAGAC
    AGTGTGGAAG CTCTTCTGAA AAAACATGAG GACTTTGAGA AGTCTCTTAG CGCCCAGGAA
    GAGAAAATCA CAGCTCTGGA TGAGTTTGCT ACAAAACTAA TTCAAAATAA CCACTATGCC
    AAGGAAGATG TGGCTACCCG CAGAGATGCT CTGCTGAGCC GCCGCAATGC ACTTCATGAA
    CGTGCACTCT ACCGCCGTAC TCAACTGGCA GACTCCTTTC ACCTGCAGCA GTTCTTTAGG
    GACTCTGACG AGCTGAAAAG TTGGATTAAT GAAAAGATGA AGACTGCAAC GGATGAGGCA
    TACAAGGATC CCTCCAACCT CCAGGGTAAG GTGCAGAAAC ACCAAGCTTT TGAGGCTGAA
    CTCTCCGCTA ACCAAAGCCG TATAGATGCC CTGGAGAATT CTGGGCAGAA ATTGATTGAT
    GTCAACCATT ATGCATCAGA TGAGGTGGCA GCACGTATGA GTGAGGTCAT CACTTTGTGG
    AAGAAGCTGC TGGAGGCCAC TGAATTGAAG GGGATCAAGC TGCGCGAAGC CAACCAGCAG
    CAGCAGTTCA ACCGTAATGT GGAGGATATA GAGCTGTGGC TGTATGAGGT TGAGGGGCAT
    TTGGCTTCAG ATGACTATGG AAAAGACCTG ACCAATGTTC AAAACTTGCA GAAAAAACAC
    GCCTTGCTGG AGGCTGATGT TGCAGCTCAC CAGGACCGTA TCGATGGCAT CACTATCCAA
    GCCCGTCAGT TCCAAGAAGC TGGTCATTTT GATGCTGACA ATATCAGGAA GAAACAGGAG
    GCCCTTGTTA ATAGATATGA AGCTTTGAAA GAACCCATGG TTGCACGCAA GCAGAAGCTG
    TCAGACTCTC TGCGTCTTCA ACAGCTTTTC AGGGATGTTG AAGATGAGGA AACCTGGATA
    AGGGAGAAGG AACCCATTGC AGCGTCCACC AACAGAGGCA AGGATTTGAT TGGAGTCCAG
    AATCTCCTGA AGAAACACCA GGCTTTGCAG GCTGAAATTG CAGGGCACGA ACCACGAATT
    AAAGCTGTTA CCCAGAAAGG AGAATCTATG ATCTCTGAAG GCCACTTTGC TTCTGATGAA
    GTGAAGGGCA AATTAAGGGA GCTGAATGAT AAGTGGGCCA CCCTGAAGAA CAAAGCATCT
    CAGCGCCGAC AGGACCTTGA AGATTCTCTG CAAGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCAGAGT CCTGGATGAG AGAGAAGGAA CCTATTGTTG GAAGCACCGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC CCTTCTGAAG AAACATGAAG CGCTTATGTC AGATCTGCGA
    GCATACGGAA GCAGTATTCA GGGACTTAGA GAGCAGGCCC AGGCCTGCAG GCAACAAGTT
    GCTCCAACTG ATGATGAAAC TGGAAAGGAG CTGGTGCTGG CATTGTATGA TTATCAAGAG
    AAGAGCCCTA GGGAGGTGAC AATGAAGAAA GGAGACATCT TGACCTTGCT AAACAGCACA
    AATAAGGATT GGTGGAAGGT GGAAGTGAAT GATCGTCAGG GCTTTGTACC TGCTGCGTAT
    GTGAAAAAGC TGGACCCAGC ACAGTCCGCA TCCCGGGAAA ACTTGCTGGA GGAACAGGGA
    AGCATCGCTC TCCGTCAGGA GCAGATCGAT GGCCAGTATC ATAATATTTT GGAGATGGGA
    GAGAAGCGCA AAGATATGTT AGAAAAGAGC TGCAAGAAGT TTATGCTGTT CCGTGAGGCA
    AATGAGCTGC AGCAGTGGAT TAACGAGAAA GAAGCATCAC TCACTAACGA AGAGGTCGGG
    GCTGACCTGG AACAAGTGGA AGTGCTGCAG AAGAAGTTTG ATGATTTCCA GAAGGATCTG
    AAAGCAAATG AGTCACGCTT GAAGGACATC AACAAGGTGG CCGACGAGCT TGAATCTGAA
    GGCCTCATTA CAGAGGAAGT CCAGCCGGTG CAGCATCAGG AGGTGTATGG GATGCAGCGG
    GATGAAGCAG ACTCCAAATC GGCATCTCCA TGGAAGTCTG TTCGCACAGC TGTGCTCGCG
    GTGGCCACCT TCAACTCTAT CAAGGACTTG AACGAGCGCT GGAAATCACT TCAACAGCTG
    GCAGAAGAAA GGAGTCAGCT TCTTGGCAGT GCCCATGAAG TACAGAGATT CCACAGAGAT
    GCTGATGAGA CAAAAGAATG GATCGAAGAG AAGAACCAGG CTTTGAATAC AGACAGCTAT
    GGACATGACT TGGCCAGTGT GCAGGCTCTG CAACGAAAAC ATGAAGGCTT TGAGAGAGAC
    CTCGCCGCTC TGGGAGATAA GGTGAACTCT TTGGGAGAAA CTGCTGAGCG CCTGATCCAG
    TCACACCCTG AGTCTGCAGA TGACATTCAG GAAAAATGCA CAGAGCTGAA TCAGGCCTGG
    AACAGCCTTG GCACCCGGGC AAACCAGCGC AAGGAGAAAC TAGGCGATTC TCATGATCTG
    CAACGCTTCC TAAGTGACTT CAGAGATCTC ATGTCCTGGA TCAATGGAAT TCGGGGCTTG
    GTGTCATCAG ATGAACTTGC TAGAGATGTG ACTGGTGCAG AGGCTTTGCT GGAACGACAC
    CAGGAGCACA GGACTGAGAT TGATGCCAGA GCAGGCACTT TTCAGGCCTT TGAGCAGTTT
    GGGCAGCAAC TTCTGGCTCG TGGTCATTAT GATAGTCCTG AAATCAAGGA GAAGCTGGAT
    ATCTTGGAAC AGGAACGGGC AAGTTTGGAA AAGGCTTGGG TGGAACGCAG AATGATGTTG
    GATCAGTGCT TGGAGCTGCA GCTCTTCAAC AGAGACTGCG AGCAGGCAGA GAACTGGATG
    GCAGCCCGTG AAGCCTTCCT GAACAGTGAG GATAAAGGGG ATTCCCTGGA CAGTGTGGAG
    GCCCTTATCA AGAAACATGA AGACTTTGAT AAAGCCATTA ATGTCCAGGA AGAGAAGATT
    GCTGCCCTCC AGGCCTTCGC CGAACAATTG ATAATTGCTG ACCACTATGC CAAAGGAGAT
    ATTGCCTCCC GTCGCAATGA AGTCCTGGAC AGGTGGCGCC GTCTCAAGGC TCAGATGATT
    GAGAAGAGAT CCAAACTTGG GGAATCTCAG ACTCTGCAGC AGTTCAGCAG GGACGTCGAT
    GAGATCGAAG CCTGGATCAG CGAGAAATTG CAGACCGCCA GTGACGAGTC CTACAAAGAC
    CCCACAAACA TCCAGAGCAA ACACCAGAAG CACCAGGCTT TCGAAGCTGA GCTCCATGCC
    AACGCTGACC GTATTCGTGG AGTAGTAGAT ATGGGGAACT CCCTTATCGA ACGAGGTGCC
    TGTGCTGGCA GCGAGGATGC GGTGAAGGCT CGCCTAAGCG CACTCGCTGA TCAATGGCAG
    TTCCTTGTCA GCAAATCCGC AGAGAAGAGC CAGAAACTGA AGGAGGCAAA CAAGCAGCAG
    AACTTTAACA CTGGCATCAA AGACTTTGAT TTCTGGTTAA CAGAGGTGGA GGCTTTGCTG
    GCATCTGAAG ACTATGGGAA GGACTTGGCA TCTGTTAACA ACCTGCTGAA GAAACACCAG
    CTTCTGGAAG CTGACATCTC TGCTCATGAG GACCGCCTTA AAGATCTTAA TGGGCAGGCT
    GATAGCCTGA TGACCAGCAG CGCCTTTGAC ACCACCCAAG TAAAGGAGAA AAGGGACGCC
    ATCAACGACC GCTTCCAGCG TATCAAAAGC ATGGCCACCG CACGCCGCAG CAGGCTGAAT
    GAGTCCCACC GTCTGCACCA GTTCTTCAGA GATCTTGATG ATGAGGAATC ATGGATCAAG
    GAAAAGAAAC TTTTGGTCGG ATCTGATGAC TATGGACGTG ACTTGACTGG AGTGCAAAAT
    CTGAGGAAGA AACACAAGAG ATTAGAAGCT GAACTGGCAG CTCATGAGCC AGCCATTCAG
    GGTGTGCTGG ACACTGGGAA GAAGCTTGCA GATGACAACA CTATTGGGAA AGATGAGATT
    CAGCAGCGAC TGGCACAGTT TCTAGAGCAC TGGAAGGAGC TGAAGGAACA AGCAGCGGCT
    CGTGGTCAGA GACTGGAGGA ATCTCTTGAG TACCAACAAT TTGTAGCCAA TGTGGAGGAA
    GAGGAAGCCT GGATTAATGA GAAGATGACT CTAGTGGCCA GTGAAGACTA TGGTGACACA
    CTGGCTGCCA TTCAGGGATT GCTGAAAAAG CACGAGGCAT TTGAGACAGA CTTTACAGTG
    CACAAAGACA GAGTACATGA TGTATGTGCC AACGGGGAAG ACCTGATACA AAAGAACAAT
    CACCATGTGG AGAATATTAG CGCCAAAATG AAGGGTCTCC GAGGGAAGGT GGCAGAACTA
    GAGAAGGCGG CTGCACAGCG CAAGGCCAAA CTTGATGAGA ATTCAGCTTT CCTGCAGTTC
    AACTGGAAGG CGGATGTTGT GGAGTCCTGG ATCGGTGAAA AAGAGAACAG CCTGAAGACT
    GATGATTATG GCAGAGACCT GTCCTCTGTG CAGACCCTTC TCACTAAGCA GGAAACATTT
    GATGCTGGGC TGCAAGCTTT CCAGCAAGAG GGCATTACCA ACATCACTGC ACTGAAAGAC
    CAGCTTCTGG CTGCAAAGCA TGTGCAGTCC CGGGCCATAG AGTCTCGTCA CGCTTCTCTG
    ATGAAGCGGT GGAACCAGTT GTTGGATAAC TCTGCTGCCC GCAAAAAGAA GTTATTGGAG
    GCCCAGGAAC ACTACAGAAA GGTTGAGGAC TTGTTCCTAA CATTTGCCAA GAAAGCCTCT
    GCCTTCAACA GCTGGTTTGA GAATGCCGAG GAGGATTTGA CTGATCCTGT TCGCTGCAAC
    TCCCTGGAGG AAATCAAGGC GCTTCGCGAG GCACATGACG CCTTCCGCAA TTCCCTCAGC
    TCCGCACAGA CAGATTTCAA CCAGCTGGCC GAACTGGACA GGCAAATAAA GGGCTACCGG
    GTAGCCTCCA ATCCCTACAC GTGGTTCACG ATGGAGGCTT TGGAGGAGAC ATGGCGCAAC
    CTGCAGAAAA TCATTAAGGA ACGAGAACTT GAGCTACAAA AAGAACAACG TAGGCAGGAA
    GAGAACGATA AGCTCCGGCA AGAGTTTGCT CAGCACGCCA ATGCTTTCCA CCAGTGGATT
    CAGGAGACAA GGACCTACCT TCTGGACGGG TCCTGTATGG TGGAAGAATC TGGAACACTG
    GAGTCACAAC TTGAAGCTAC AAAGAGAAAG CATCAGGAGA TCCGGGCCAT GAGGAGTCAA
    CTGAAGAAGA TAGAAGATCT TGGCGCAGCC ATGGAAGAGG CCCTGATCTT GGATAATAAA
    TACACAGAAC ACAGCACTGT TGGGCTGGCA CAGCAATGGG ACCAACTGGA TCAGCTAGGC
    ATGAGAATGC AACACAACCT AGAGCAACAG ATCCAGGCCA GAAACACAAC TGGGGTCACA
    GAGGAAGCAC TCAAAGAATT CAGCATGATG TTCAAGCATT TCGACAAAGA CAAGTCTGGC
    CGCCTCAACC ACCAAGAATT CAAATCTTGT CTGCGGTCCC TTGGCTATGA TCTGCCCATG
    GTAGAAGAAG GAGAACCAGA CCCAGAGTTT GAAGCCATTT TGGACACTGT AGATCCCAAC
    AGAGATGGGC ACGTTTCTCT ACAGGAGTAC ATGGCTTTCA TGATTAGCAG AGAAACTGAA
    AACGTCAAGT CCAGTGAGGA GATAGAGAGC GCCTTCCGAG CACTGAGTAC AGACCAGAAA
    CCTTATGTCA CAAAGGAAGA ACTATACCAG AACCTGACAC GCGAACAGGC AGATTACTGC
    ATCTCGCATA TGAAACCCTA CATGGACAGC AAGGGCCGCG AACTGCCCTC TGCATACGAC
    TACGTGGAAT TCACGCGCTC GCTATTTGTG AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.