SPTAN1 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPTAN1
Entrez Gene ID	374234
Full Name	spectrin alpha, non-erythrocytic 1
Synonyms	SPECA
General protein information	Preferred Names spectrin alpha, non-erythrocytic 1 Names spectrin alpha chain, non-erythrocytic 1 alpha-II spectrin alpha-spectrin fodrin alpha chain nonerythroid alpha-spectrin spectrin alpha chain, brain spectrin, alpha, non-erythrocytic 1 (alpha-fodrin) spectrin, non-erythroid alpha chain
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015279284.1	XP_015134770.1	spectrin alpha chain, non-erythrocytic 1 isoform X3
XM_015279287.1	XP_015134773.1	spectrin alpha chain, non-erythrocytic 1 isoform X6
XM_015279288.1	XP_015134774.1	spectrin alpha chain, non-erythrocytic 1 isoform X7
XM_015279289.1	XP_015134775.1	spectrin alpha chain, non-erythrocytic 1 isoform X8
XM_015279285.2	XP_015134771.1	spectrin alpha chain, non-erythrocytic 1 isoform X4
XM_015279287.2	XP_015134773.1	spectrin alpha chain, non-erythrocytic 1 isoform X6
XM_015279289.2	XP_015134775.1	spectrin alpha chain, non-erythrocytic 1 isoform X8
XM_015279290.2	XP_015134776.1	spectrin alpha chain, non-erythrocytic 1 isoform X9
NM_001042538.1	NP_001036003.1	spectrin alpha chain, non-erythrocytic 1
XM_015279286.1	XP_015134772.1	spectrin alpha chain, non-erythrocytic 1 isoform X5
XM_015279293.1	XP_015134779.1	spectrin alpha chain, non-erythrocytic 1 isoform X12
XM_015279291.2	XP_015134777.1	spectrin alpha chain, non-erythrocytic 1 isoform X10
XM_015279292.2	XP_015134778.1	spectrin alpha chain, non-erythrocytic 1 isoform X11
XM_015279285.1	XP_015134771.1	spectrin alpha chain, non-erythrocytic 1 isoform X4
XM_015279294.1	XP_015134780.1	spectrin alpha chain, non-erythrocytic 1 isoform X13
XM_015279283.1	XP_015134769.1	spectrin alpha chain, non-erythrocytic 1 isoform X2
XM_015279291.1	XP_015134777.1	spectrin alpha chain, non-erythrocytic 1 isoform X10
XM_015279282.1	XP_015134768.1	spectrin alpha chain, non-erythrocytic 1 isoform X1
XM_015279284.2	XP_015134770.1	spectrin alpha chain, non-erythrocytic 1 isoform X3
XM_015279288.2	XP_015134774.1	spectrin alpha chain, non-erythrocytic 1 isoform X7
XM_015279293.2	XP_015134779.1	spectrin alpha chain, non-erythrocytic 1 isoform X12
XM_015279294.2	XP_015134780.1	spectrin alpha chain, non-erythrocytic 1 isoform X13
XM_015279290.1	XP_015134776.1	spectrin alpha chain, non-erythrocytic 1 isoform X9
XM_015279292.1	XP_015134778.1	spectrin alpha chain, non-erythrocytic 1 isoform X11
XM_015279286.2	XP_015134772.1	spectrin alpha chain, non-erythrocytic 1 isoform X5

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Sptan1	NP_741984.2
Danio rerio (zebrafish)	spna2	NP_001091958.1
Caenorhabditis elegans (roundworm)	spc-1	NP_001257000.1
Xenopus tropicalis (tropical clawed frog)	sptan1	NP_001090674.1
Canis lupus familiaris (dog)	SPTAN1	XP_005625341.1
Bos taurus (cattle)	SPTAN1	NP_001107628.1
Mus musculus (house mouse)	Sptan1	NP_001171138.1
Gallus gallus (chicken)	SPTAN1	NP_001036003.1
Homo sapiens (human)	SPTAN1	NP_001123910.1
	SPTAN1	XP_002800011.1
Drosophila melanogaster (fruit fly)	alpha-Spec	NP_001261286.1
Pan troglodytes (chimpanzee)	SPTAN1	XP_003312345.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006686	XP_316724.1

Gene Ontology
Bibliography

Related articles in PubMed

Non-equilibrium hydrogen exchange for determination of H-bond strength and water accessibility in solid proteins.
Grohe K, Movellan KT, Vasa SK, Giller K, Becker S, Linser R
Journal of biomolecular NMR68(1)7-17(2017 May)

Thermal stability of chicken brain ?-spectrin repeat 17: a spectroscopic study.
Brenner AK, Kieffer B, Trav? G, Fr?ystein NA, Raae AJ
Journal of biomolecular NMR53(2)71-83(2012 Jun)

Different members of a simple three-helix bundle protein family have very different folding rate constants and fold by different mechanisms.
Wensley BG, G?rtner M, Choo WX, Batey S, Clarke J
Journal of molecular biology390(5)1074-85(2009 Jul)

Distinguishing specific and nonspecific interdomain interactions in multidomain proteins.
Randles LG, Batey S, Steward A, Clarke J
Biophysical journal94(2)622-8(2008 Jan)

The high-resolution NMR structure of the R21A Spc-SH3:P41 complex: understanding the determinants of binding affinity by comparison with Abl-SH3.
Casares S, Ab E, Eshuis H, Lopez-Mayorga O, van Nuland NA, Conejero-Lara F
BMC structural biology722(2007 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Tracking of the NMR HN peak intensities for 2 weeks reports on site-specific hydrogen bond strength and also likely reflects water accessibility in a qualitative manner.
Title: Non-equilibrium hydrogen exchange for determination of H-bond strength and water accessibility in solid proteins.

Spectroscopic study of the thermal stability of chicken brain alpha-spectrin repeat 17.
Title: Thermal stability of chicken brain ?-spectrin repeat 17: a spectroscopic study.

The rate-limiting transition state for R15 folding is investigated using protein engineering methods (Phi-value analysis) and compared with previously completed analyses of R16 and R17 (15th, 16th repeats of alpha-spectrin).
Title: Different members of a simple three-helix bundle protein family have very different folding rate constants and fold by different mechanisms.

Spectrin-titin domain pairs of both spectrin R16 and R17 with a single titin I27 domain at either the N- or the C-terminus were created and found that spectrin domains are significantly stabilized, by nonnative interactions at the C-terminus only.
Title: Distinguishing specific and nonspecific interdomain interactions in multidomain proteins.

The high-resolution structure of the complex between the R21A mutant of Spc-SH3 and p41 derived from nuclear magnetic resonance data, is presented.
Title: The high-resolution NMR structure of the R21A Spc-SH3:P41 complex: understanding the determinants of binding affinity by comparison with Abl-SH3.

The following SPTAN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPTAN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa43308	XM_015279282.1 Latest version!	Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OGa43309	XM_015279283.1 Latest version!	Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OGa43310	XM_015279284.2 Latest version!	Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OGa43311	XM_015279285.2 Latest version!	Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OGa43312	XM_015279286.2 Latest version!	Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OGa43313	XM_015279287.2 Latest version!	Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OGa43314	XM_015279288.2 Latest version!	Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OGa43315	XM_015279289.2 Latest version!	Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OGa43316	XM_015279290.2 Latest version!	Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OGa43317	XM_015279291.2 Latest version!	Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OGa43318	XM_015279292.2 Latest version!	Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OGa43319	XM_015279293.2 Latest version!	Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OGa43320	XM_015279294.2 Latest version!	Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OGa27423	NM_001042538.1 Latest version!	Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
View Old Accession Versions >>
OGa43313	XM_015279287.1	Gallus gallus spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OGa43315	XM_015279289.1	Gallus gallus spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OGa43311	XM_015279285.1	Gallus gallus spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OGa43317	XM_015279291.1	Gallus gallus spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OGa43310	XM_015279284.1	Gallus gallus spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OGa43314	XM_015279288.1	Gallus gallus spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OGa43319	XM_015279293.1	Gallus gallus spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OGa43320	XM_015279294.1	Gallus gallus spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OGa43316	XM_015279290.1	Gallus gallus spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OGa43318	XM_015279292.1	Gallus gallus spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OGa43312	XM_015279286.1	Gallus gallus spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPTAN1 ( XM_015279282.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015134768.1 Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa43308
Clone ID Related Accession (Same CDS sequence)	XM_015279282.1
Accession Version	XM_015279282.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG 
TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC 
TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT 
GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT 
GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG 
ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAT CTTTCACGCC TTACAGGACT 
GCACAGCGCC CAATAGTAAC AGCCACCCAA GTTGGGTCCC ACATACGAAC TCGTCTACAG 
GAGCTGCACC GACTATGGGA GTTACTGCTG GAAAAAATGA GAGAGAAGGG AGTGAAGCTG 
TTGCAAGCAC AGAAGCTGGT GCAATTCTTA CGGGAATGTG AAGATGTCAT GGACTGGATC 
AATGACAAGG AAGCAATAGT GACCTCAGAA GAGCTTGGAC AGGACTTAGA GCATGTTGAG 
GTTTTGCAGA AGAAATTTGA AGAGTTCCAA ACAGATCTGG CAGCTCATGA GGAGAGAGTG 
AATGAAGTGA ACCAGTTCGC TGGCAAACTT ATCCAGGAAC AGCACCCTGA GGAGGAACTT 
ATAAAGTCCA AACAGGATGA AGTAAATGCA AGCTGGCAGC GTCTTAAGGG GCTTGCCCAA 
CAGAGGCAAG GAAAACTCTT TGGGGCAGCT GAAGTTCAGC GCTTCAACAG GGATGTGGAT 
GAAACTATCA GCTGGATTAA GGAGAAAGGG CAGTTGATGG CCTCAGATGA CTTTGGCAGA 
GACTTGGCCA GCGTGCAAGC TTTACTACGT AAGCATGAAG GCCTGGAAAG AGATCTTGCA 
GCTTTGGAGG ATAAGGTTAA GGCCCTCTGT GCAGAAGCTG ACCGTTTGCA GCAGTCTCAC 
CCTATAAATG CTTCTCAAAT TCAAGTGAAA CGGGAGGAAC TGATTGCCAA CTGGGAGCAG 
ATCCGAACTC TGGCAGCAGA GAGGCATGCT CGCCTTAATG ACTCCTACAG GTTGCAGCGC 
TTTCTTGCAG ATTTCCGGGA CCTCACTAGC TGGGTAACTG AGATGAAGGC TCTGATAAAT 
GCTGATGAGC TTGCCAATGA TGTGGCTGGA GCAGAAGCCC TTCTAGATAG ACATCAGGAA 
CATAAGGGAG AAATTGATGC TCATGAGGAT AGCTTCAGAT CTGCTGATGA GTCTGGGCAG 
GCTTTGCTTG CAGCTGGGCA CTATGCTTCT GATGAAGTTA AAGAAAAGCT GACTATCCTC 
TCAGATGAAA GATCAGCCTT GCTGGAACTA TGGGAGCTTC GCAGGCAACA GTATGAGCAG 
TGCATGGATC TGCAGCTTTT CTACAGAGAT ACTGAACAAG TTGACAACTG GATGAGCAAA 
CAAGAAGCTT TTCTGCTGAA CGAAGACCTT GGTGATTCTC TGGATAGCGT GGAGGCTCTT 
CTAAAGAAGC ATGAAGATTT TGAGAAATCC CTAAGTGCTC AAGAGGAGAA GATCACAGCA 
CTGGATGAGT TTGCTACTAA ACTGATTCAA AATAACCATT ATGCCATGGA TGACGTTGCT 
ACGCGCAGAG ATGCTCTTCT GAGTCGCCGA AATGCTCTTC ATGAAAGAGC CATGAAGCGC 
CGTGCCCAAC TGGCAGACTC TTTCCATCTC CAGCAGTTTT TCAGGGACTC TGATGAACTG 
AAGAGTTGGG TTAATGAAAA AATGAAAACT GCTACCGATG AGGCTTACAA GGATCCATCC 
AACTTGCAAG GTAAAGTTCA GAAGCATCAG GCTTTTGAAG CAGAGCTTTC TGCTAATCAG 
AGTCGTATTG ATGCACTGGA GAAAGCTGGC CAGAAGTTGA TTGATGTCAA TCACTATGCA 
TCTGATGAGG TGGCAGCTCG CATGAATGAA GTCATCAGCT TGTGGAAGAA ACTTCTGGAA 
GCCACCGAGC TCAAAGGTAT AAAGCTGCGT GAAGCCAATC AACAGCAGCA ATTTAATCGC 
AATGTAGAAG ACATTGAGTT GTGGCTGTAT GAAGTGGAGG GTCACTTGGC TTCTGATGAT 
TATGGAAAAG ATCTTACCAG CGTTCAGAAT CTTCAGAAGA AACATGCCCT GCTAGAGGCA 
GATGTTGCTG CCCATCAGGA TCGGATAGAT GGCATTACCA TCCAGGCACG CCAGTTCCAA 
GATGCTGGGC ACTTTGATGC TGACAATATC AAGAAGAAAC AAGAAGCTTT AGTAGCTCGT 
TATGAAGCTC TGAAGGATCC TATGGTGGCT CGCAAGCAGA AACTTGCAGA TTCTCTTCGC 
CTGCAGCAGC TTTTCCGTGA CATCGAGGAT GAAGAGACCT GGATCAGGGA AAAGGAACCT 
ATTGCAGCCT CAACAAACCG AGGCAAGGAC TTAATTGGTG TCCAGAATCT GCTAAAGAAG 
CACCAGGCTT TGCAGGCAGA AATTGCAGGC CATGAGCCTC GCATTAAAGC AGTCACACAA 
AAAGGAAATG CGATGGTGGA AGAGGGACAT TTTGCTGCTG AAGATGTGAA GATCAAATTG 
AATGAACTAA ACCAAAAATG GGACTCTCTG AAAGCCAAGG CATCTCAACG GCGACAGGAT 
CTAGAGGATT CTCTGCAAGC TCAGCAGTAT TTTGCTGATG CTAATGAGGC AGAATCATGG 
ATGAGGGAAA AGGAGCCCAT TGTAGGCAGC ACGGATTATG GAAAGGATGA AGACTCTGCT 
GAGGCTCTCC TGAAGAAGCA TGAAGCTTTG ATGTCTGATC TTTCTGCTTA CGGCAGTAGC 
ATACAGGCAT TAAGGGAACA GGCCCAGTCC TGCAGGCAAC AAGTTGCTCC CACTGATGAT 
GAAACTGGAA AAGAGCTTGT GCTAGCACTC TATGATTACC AAGAGAAGAG TCCTCGGGAG 
GTGACTATGA AGAAAGGAGA TATTCTAACC CTGCTCAACA GCACCAACAA GGACTGGTGG 
AAGGTTGAAG TTAACGATCG TCAGGGCTTT GTACCAGCTG CCTATGTGAA AAAACTAGAT 
CCTGCCCAGT CTGCATCCCG AGAGAATCTT CTAGAAGAGC AAGGCAGCAT AGCATTGCGA 
CAGGAGCAAA TTGACAACCA GACTCTCATT ACTAAGGAAG TCGGCAGTGT ATCTCTGCGT 
ATGAAACAGG TCGAAGAACT GTATCATTCC CTTCTTGAGC TGGGAGAAAA ACGTAAAGGC 
ATGCTAGAAA AAAGCTGCAA GAAGTTTATG CTTTTCCGTG AGGCTAATGA GCTTCAACAG 
TGGATCAATG AGAAGGAAGC TGCGCTCACT AATGAGGAAG TGGGTGCTGA TTTGGAGCAG 
GTGGAGGTGC TACAGAAGAA GTTTGATGAT TTTCAGAAGG ATTTAAAAGC TAATGAGTCA 
CGACTGAAGG ACATAAACAA GGTTGCAAAT GACCTGGAGT CTGAAGGGCT GATGGCAGAG 
GAAGTGCAAG CAGTAGAGCA CCAGGAAGTC TATGGAATGA TGCCCAGAGA TGAAACTGAT 
TCTAAGACAG CCTCTCCTTG GAAGTCTGCA CGTATGATGG TACACACAGT GGCAACGTTT 
AACTCAATCA AGGAACTGAA TGAACGCTGG AGATCCCTGC AGCAGTTGGC AGAGGAGCGA 
AGCCAGCTGT TGGGCAGCGC TCATGAAGTC CAGAGGTTCC ACAGAGATGC TGATGAAACA 
AAAGAATGGA TAGAAGAGAA GAATCAAGCA TTAAATACCG ACAACTATGG ACATGACTTG 
GCCAGTGTGC AGGCTCTGCA GCGCAAGCAT GAAGGCTTTG AGAGAGACTT GGCAGCTCTT 
GGAGACAAGG TGAATTCTCT TGGTGAAACT GCCCAGCGTC TGATCCAGTC ACATCCAGAA 
TCAGCTGAAG ATCTTCAAGA AAAATGTACT GAGTTGAACC AAGCCTGGAG TAGTCTGGGA 
AAACGTGCTG ACCAACGCAA AGAGAAGCTT GGAGATTCTC ATGACCTGCA GCGTTTCCTC 
AGTGATTTTA GAGACCTTAT GTCTTGGATC AATGGAATCC GGGGTCTGGT CTCCTCAGAT 
GAACTTGCAA AAGATGTGAC TGGAGCTGAA GCTCTGTTGG AAAGGCATCA GGAGCACCGT 
ACTGAAATAG ATGCACGGGC TGGCACTTTT CAGGCATTTG AACAGTTTGG ACAGCAGCTT 
CTGGCTCGTG GACACTATGC CAGCCCAGAG ATTAAGGAGA AACTGGATAT TCTAGATCAA 
GAACGGACAG ACCTAGAGAA GGCCTGGGTC CAGCGCAGAA TGATGCTAGA CCAGTGCTTA 
GAACTACAGC TGTTTCATCG GGATTGTGAA CAAGCTGAAA ACTGGATGGC TGCCCGAGAG 
GCGTTCCTAA ATACAGAAGA CAAAGGAGAC TCCTTAGACA GCGTGGAGGC ACTCATCAAG 
AAGCATGAAG ATTTCGATAA AGCAATCAAT GTCCAGGAAG AGAAAATTGC TGTCTTGCAG 
TCTTTTGCTG ACCAACTGAT TGCTGCTGAT CATTATGCAA AAGGAGTCAT TGCTAACAGA 
CGCAATGAGG TTCTGGACAG GTGGCGTCGT CTGAAGGCTC AGATGATTGA GAAGAGATCT 
AAGCTGGGAG AATCTCAGAC CCTCCAACAG TTCAGTCGTG ATGTTGATGA AATAGAAGCT 
TGGATCAGTG AAAAGCTTCA AACTGCAAGT GATGAGTCAT ATAAGGATCC CACAAACATC 
CAGCTTTCCA AACTGCTGAG CAAGCACCAG AAGCACCAAG CCTTTGAAGC TGAGCTCCAC 
GCCAACGCAG ATCGGATTCG TGGAGTCATT GAAATGGGGA ACTCTCTTAT TGAAAGAGGA 
GCGTGTGCTG GCAGCGAGGA TGCTGTGAAG GCTCGGCTGG CTGCCCTGGC TGACCAATGG 
GAGTTCCTGG TCCAGAAGTC ATCAGAGAAG AGTCAGAAAC TGAAAGAAGC AAATAAACAG 
CAGAATTTCA ATACCGGCAT CAAGGACTTT GATTTCTGGC TTTCAGAGGT GGAAGCTTTG 
TTGGCATCTG AAGACTATGG GAAGGACTTG GCATCAGTGA ACAACCTTCT GAAGAAGCAC 
CAATTACTGG AAGCTGATAT ATCTGCTCAT GAGGATCGGC TGAAGGACCT GAACAGCCAG 
GCTGACAGTT TGATGACCAG CAGTGCTTTC GATACCTCCC AAGTAAAGGA TAAACGTGAA 
ACTATAAATG GGCGCTTCCA GAGAATCAAG AGCATGGCAG CTGCCCGCCG TGCGAAGCTC 
AACGAGTCGC ACCGCTTGCA TCAGTTCTTC CGTGACATGG ATGATGAGGA GTCCTGGATC 
AAAGAGAAGA AACTGTTGGT GAGCTCAGAG GACTATGGCA GAGACCTGAC TGGTGTGCAG 
AACCTGAGGA AGAAACATAA GCGCTTGGAA GCAGAATTAG CTGCCCATGA ACCTGCTATC 
CAGGGTGTTC TAGACACTGG CAAGAAGCTT TCAGATGATA ACACAATTGG GAAGGAGGAG 
ATACAGCAAA GACTGGCTCA GTTTGTGGAC CACTGGAAAG AGTTAAAACA GCTGGCAGCT 
GCTCGGGGGC AGCGTCTGGA GGAGTCCCTG GAGTACCAAC AGTTTGTAGC AAATGTTGAG 
GAAGAGGAGG CATGGATCAA CGAGAAAATG ACATTGGTAG CCAGTGAGGA TTATGGAGAC 
ACACTTGCTG CTATCCAGGG CTTGCTGAAG AAGCATGAAG CATTCGAGAC TGACTTTACT 
GTCCACAAAG ACAGAGTGAA TGATGTTTGC GCTAATGGAG AGGATCTCAT TAAAAAGAAT 
AATCACCATG TGGAGAACAT TACTGCTAAG ATGAAGGGCC TCAAGGGCAA GGTATCAGAT 
CTGGAGAAAG CCGCAGCTCA GAGGAAAGCC AAACTGGATG AGAACTCTGC CTTCCTCCAG 
TTCAACTGGA AAGCAGATGT GGTGGAGTCA TGGATAGGTG AGAAGGAAAA CAGTCTGAAG 
ACAGATGATT ATGGCCGTGA CCTCTCCTCT GTGCAAACAC TACTCACCAA ACAGGAAACC 
TTTGATGCTG GACTTCAGGC TTTCCAGCAG GAGGGAATTG CAAACATCAC TGCTCTGAAA 
GACCAGCTAC TGGCAGCCAA ACATATCCAA TCAAAGGCCA TTGAGGTTCG TCACGCTTCC 
TTGATGAAAC GCTGGAATCA GCTGCTTGCT AATTCTGCAG CCAGGAAAAA GAAACTCTTG 
GAGGCTCAGG AGCACTTCAG AAAGGTTGAA GATCTCTTCT TGACTTTTGC CAAGAAGGCC 
TCTGCCTTCA ACAGTTGGTT TGAGAATGCT GAGGAGGACC TGACGGATCC CGTGCGCTGC 
AATTCCCTGG AAGAAATCAA AGCACTGCGA GAAGCCCACG ATGCGTTCCG TTCCTCACTT 
AGCTCTGCCC AAGCTGACTT CAACCAGCTG GCAGAGCTTG ATCGGCAGAT CAAGAGCTTC 
CGTGTAGCCT CCAACCCGTA CACTTGGTTT ACTATGGAGG CTCTTGAAGA AACTTGGAGG 
AATCTGCAGA AAATTATCAA GGAGCGTGAA TTGGAGTTGC AGAAGGAGCA GCGGAGGCAG 
GAAGAAAATG ACAAGTTGCG CCAGGAGTTT GCTCAGCATG CTAATGCCTT CCACCAGTGG 
ATTCAGGAGA CAAGGACTTA CCTGCTAGAT GGCATAGCCT ATCGTCGTGT CATTCGTGTC 
TATCAGTATG AAGTTGGGGA TGATCTATCT GGAAGGTCCT GCATGGTGGA GGAGTCGGGA 
ACACTGGAAT CCCAGCTGGA AGCTACTAAA CGCAAGCACC AGGAGATCCG GGCTATGAGG 
AGCCAGCTGA AGAAGATTGA GGACCTTGGA GCAGCCATGG AAGAGGCACT TATCTTGGAC 
AACAAATACA CAGAGCACAG CACCGTGGGG CTGGCGCAGC AGTGGGACCA GCTTGACCAG 
CTGGGAATGA GAATGCAGCA CAATCTGGAA CAGCAGATTC AAGCTCGAAA CACAACTGGA 
GTCACAGAGG AGGCCCTGAA GGAGTTCAGC ATGATGTTCA AGCACTTTGA CAAGGACAAA 
TCTGGACGTC TTAATCACCA GGAGTTTAAG TCTTGCTTGC GCTCCCTTGG CTATGACCTG 
CCCATGGTTG AGGAAGGAGA GCCTGACCCC GAATTTGAGT CTATTCTTGA CACTGTTGAT 
CCCAACAGGG ATGGCCACGT CTCGCTGCAG GAGTACATGG CGTTCATGAT CAGCAGGGAA 
ACAGAGAACG TGAAATCCAG CGAGGAGATT GAGAGTGCTT TCCGTGCCCT CAGCTCGGAG 
GGGAAGCCTT ACGTGACCAA GGAGGAGCTC TACCAGAACC TGACCCGGGA GCAGGCCGAC 
TACTGCATTT CTCACATGAA GCCCTACATG GATGGCAAGG GCAGAGAACT TCCTTCTGCC 
TACGACTACA TAGAATTTAC ACGTTCACTC TTTGTGAATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015134768.1
CDS	19..7620
Translation	MLSSFRKVKVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSSLRRQKLEDSYRFQFFQRDADELEKWIQEKLQIASDENYKDPSNLQGKLQKHQAFEAEVQANSGAIVKLDETGNQMINEGHFASETIRSFTPYRTAQRPIVTATQVGSHIRTRLQELHRLWELLLEKMREKGVKLLQAQKLVQFLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDLAAHEERVNEVNQFAGKLIQEQHPEEELIKSKQDEVNASWQRLKGLAQQRQGKLFGAAEVQRFNRDVDETISWIKEKGQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPINASQIQVKREELIANWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFRSADESGQALLAAGHYASDEVKEKLTILSDERSALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMDDVATRRDALLSRRNALHERAMKRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYASDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTSVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDADNIKKKQEALVARYEALKDPMVARKQKLADSLRLQQLFRDIEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKIKLNELNQKWDSLKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPTDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTLITKEVGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTNEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVANDLESEGLMAEEVQAVEHQEVYGMMPRDETDSKTASPWKSARMMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAQRLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKEKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLARGHYASPEIKEKLDILDQERTDLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAVLQSFADQLIAADHYAKGVIANRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIEMGNSLIERGACAGSEDAVKARLAALADQWEFLVQKSSEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRETINGRFQRIKSMAAARRAKLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVDHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCANGEDLIKKNNHHVENITAKMKGLKGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHIQSKAIEVRHASLMKRWNQLLANSAARKKKLLEAQEHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGIAYRRVIRVYQYEVGDDLSGRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFESILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCISHMKPYMDGKGRELPSAYDYIEFTRSLFVN

Target ORF information:

RefSeq Version	XM_015279282.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279282.1

ORF Insert Sequence:

ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG 
TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC 
TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT 
GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT 
GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG 
ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAT CTTTCACGCC TTACAGGACT 
GCACAGCGCC CAATAGTAAC AGCCACCCAA GTTGGGTCCC ACATACGAAC TCGTCTACAG 
GAGCTGCACC GACTATGGGA GTTACTGCTG GAAAAAATGA GAGAGAAGGG AGTGAAGCTG 
TTGCAAGCAC AGAAGCTGGT GCAATTCTTA CGGGAATGTG AAGATGTCAT GGACTGGATC 
AATGACAAGG AAGCAATAGT GACCTCAGAA GAGCTTGGAC AGGACTTAGA GCATGTTGAG 
GTTTTGCAGA AGAAATTTGA AGAGTTCCAA ACAGATCTGG CAGCTCATGA GGAGAGAGTG 
AATGAAGTGA ACCAGTTCGC TGGCAAACTT ATCCAGGAAC AGCACCCTGA GGAGGAACTT 
ATAAAGTCCA AACAGGATGA AGTAAATGCA AGCTGGCAGC GTCTTAAGGG GCTTGCCCAA 
CAGAGGCAAG GAAAACTCTT TGGGGCAGCT GAAGTTCAGC GCTTCAACAG GGATGTGGAT 
GAAACTATCA GCTGGATTAA GGAGAAAGGG CAGTTGATGG CCTCAGATGA CTTTGGCAGA 
GACTTGGCCA GCGTGCAAGC TTTACTACGT AAGCATGAAG GCCTGGAAAG AGATCTTGCA 
GCTTTGGAGG ATAAGGTTAA GGCCCTCTGT GCAGAAGCTG ACCGTTTGCA GCAGTCTCAC 
CCTATAAATG CTTCTCAAAT TCAAGTGAAA CGGGAGGAAC TGATTGCCAA CTGGGAGCAG 
ATCCGAACTC TGGCAGCAGA GAGGCATGCT CGCCTTAATG ACTCCTACAG GTTGCAGCGC 
TTTCTTGCAG ATTTCCGGGA CCTCACTAGC TGGGTAACTG AGATGAAGGC TCTGATAAAT 
GCTGATGAGC TTGCCAATGA TGTGGCTGGA GCAGAAGCCC TTCTAGATAG ACATCAGGAA 
CATAAGGGAG AAATTGATGC TCATGAGGAT AGCTTCAGAT CTGCTGATGA GTCTGGGCAG 
GCTTTGCTTG CAGCTGGGCA CTATGCTTCT GATGAAGTTA AAGAAAAGCT GACTATCCTC 
TCAGATGAAA GATCAGCCTT GCTGGAACTA TGGGAGCTTC GCAGGCAACA GTATGAGCAG 
TGCATGGATC TGCAGCTTTT CTACAGAGAT ACTGAACAAG TTGACAACTG GATGAGCAAA 
CAAGAAGCTT TTCTGCTGAA CGAAGACCTT GGTGATTCTC TGGATAGCGT GGAGGCTCTT 
CTAAAGAAGC ATGAAGATTT TGAGAAATCC CTAAGTGCTC AAGAGGAGAA GATCACAGCA 
CTGGATGAGT TTGCTACTAA ACTGATTCAA AATAACCATT ATGCCATGGA TGACGTTGCT 
ACGCGCAGAG ATGCTCTTCT GAGTCGCCGA AATGCTCTTC ATGAAAGAGC CATGAAGCGC 
CGTGCCCAAC TGGCAGACTC TTTCCATCTC CAGCAGTTTT TCAGGGACTC TGATGAACTG 
AAGAGTTGGG TTAATGAAAA AATGAAAACT GCTACCGATG AGGCTTACAA GGATCCATCC 
AACTTGCAAG GTAAAGTTCA GAAGCATCAG GCTTTTGAAG CAGAGCTTTC TGCTAATCAG 
AGTCGTATTG ATGCACTGGA GAAAGCTGGC CAGAAGTTGA TTGATGTCAA TCACTATGCA 
TCTGATGAGG TGGCAGCTCG CATGAATGAA GTCATCAGCT TGTGGAAGAA ACTTCTGGAA 
GCCACCGAGC TCAAAGGTAT AAAGCTGCGT GAAGCCAATC AACAGCAGCA ATTTAATCGC 
AATGTAGAAG ACATTGAGTT GTGGCTGTAT GAAGTGGAGG GTCACTTGGC TTCTGATGAT 
TATGGAAAAG ATCTTACCAG CGTTCAGAAT CTTCAGAAGA AACATGCCCT GCTAGAGGCA 
GATGTTGCTG CCCATCAGGA TCGGATAGAT GGCATTACCA TCCAGGCACG CCAGTTCCAA 
GATGCTGGGC ACTTTGATGC TGACAATATC AAGAAGAAAC AAGAAGCTTT AGTAGCTCGT 
TATGAAGCTC TGAAGGATCC TATGGTGGCT CGCAAGCAGA AACTTGCAGA TTCTCTTCGC 
CTGCAGCAGC TTTTCCGTGA CATCGAGGAT GAAGAGACCT GGATCAGGGA AAAGGAACCT 
ATTGCAGCCT CAACAAACCG AGGCAAGGAC TTAATTGGTG TCCAGAATCT GCTAAAGAAG 
CACCAGGCTT TGCAGGCAGA AATTGCAGGC CATGAGCCTC GCATTAAAGC AGTCACACAA 
AAAGGAAATG CGATGGTGGA AGAGGGACAT TTTGCTGCTG AAGATGTGAA GATCAAATTG 
AATGAACTAA ACCAAAAATG GGACTCTCTG AAAGCCAAGG CATCTCAACG GCGACAGGAT 
CTAGAGGATT CTCTGCAAGC TCAGCAGTAT TTTGCTGATG CTAATGAGGC AGAATCATGG 
ATGAGGGAAA AGGAGCCCAT TGTAGGCAGC ACGGATTATG GAAAGGATGA AGACTCTGCT 
GAGGCTCTCC TGAAGAAGCA TGAAGCTTTG ATGTCTGATC TTTCTGCTTA CGGCAGTAGC 
ATACAGGCAT TAAGGGAACA GGCCCAGTCC TGCAGGCAAC AAGTTGCTCC CACTGATGAT 
GAAACTGGAA AAGAGCTTGT GCTAGCACTC TATGATTACC AAGAGAAGAG TCCTCGGGAG 
GTGACTATGA AGAAAGGAGA TATTCTAACC CTGCTCAACA GCACCAACAA GGACTGGTGG 
AAGGTTGAAG TTAACGATCG TCAGGGCTTT GTACCAGCTG CCTATGTGAA AAAACTAGAT 
CCTGCCCAGT CTGCATCCCG AGAGAATCTT CTAGAAGAGC AAGGCAGCAT AGCATTGCGA 
CAGGAGCAAA TTGACAACCA GACTCTCATT ACTAAGGAAG TCGGCAGTGT ATCTCTGCGT 
ATGAAACAGG TCGAAGAACT GTATCATTCC CTTCTTGAGC TGGGAGAAAA ACGTAAAGGC 
ATGCTAGAAA AAAGCTGCAA GAAGTTTATG CTTTTCCGTG AGGCTAATGA GCTTCAACAG 
TGGATCAATG AGAAGGAAGC TGCGCTCACT AATGAGGAAG TGGGTGCTGA TTTGGAGCAG 
GTGGAGGTGC TACAGAAGAA GTTTGATGAT TTTCAGAAGG ATTTAAAAGC TAATGAGTCA 
CGACTGAAGG ACATAAACAA GGTTGCAAAT GACCTGGAGT CTGAAGGGCT GATGGCAGAG 
GAAGTGCAAG CAGTAGAGCA CCAGGAAGTC TATGGAATGA TGCCCAGAGA TGAAACTGAT 
TCTAAGACAG CCTCTCCTTG GAAGTCTGCA CGTATGATGG TACACACAGT GGCAACGTTT 
AACTCAATCA AGGAACTGAA TGAACGCTGG AGATCCCTGC AGCAGTTGGC AGAGGAGCGA 
AGCCAGCTGT TGGGCAGCGC TCATGAAGTC CAGAGGTTCC ACAGAGATGC TGATGAAACA 
AAAGAATGGA TAGAAGAGAA GAATCAAGCA TTAAATACCG ACAACTATGG ACATGACTTG 
GCCAGTGTGC AGGCTCTGCA GCGCAAGCAT GAAGGCTTTG AGAGAGACTT GGCAGCTCTT 
GGAGACAAGG TGAATTCTCT TGGTGAAACT GCCCAGCGTC TGATCCAGTC ACATCCAGAA 
TCAGCTGAAG ATCTTCAAGA AAAATGTACT GAGTTGAACC AAGCCTGGAG TAGTCTGGGA 
AAACGTGCTG ACCAACGCAA AGAGAAGCTT GGAGATTCTC ATGACCTGCA GCGTTTCCTC 
AGTGATTTTA GAGACCTTAT GTCTTGGATC AATGGAATCC GGGGTCTGGT CTCCTCAGAT 
GAACTTGCAA AAGATGTGAC TGGAGCTGAA GCTCTGTTGG AAAGGCATCA GGAGCACCGT 
ACTGAAATAG ATGCACGGGC TGGCACTTTT CAGGCATTTG AACAGTTTGG ACAGCAGCTT 
CTGGCTCGTG GACACTATGC CAGCCCAGAG ATTAAGGAGA AACTGGATAT TCTAGATCAA 
GAACGGACAG ACCTAGAGAA GGCCTGGGTC CAGCGCAGAA TGATGCTAGA CCAGTGCTTA 
GAACTACAGC TGTTTCATCG GGATTGTGAA CAAGCTGAAA ACTGGATGGC TGCCCGAGAG 
GCGTTCCTAA ATACAGAAGA CAAAGGAGAC TCCTTAGACA GCGTGGAGGC ACTCATCAAG 
AAGCATGAAG ATTTCGATAA AGCAATCAAT GTCCAGGAAG AGAAAATTGC TGTCTTGCAG 
TCTTTTGCTG ACCAACTGAT TGCTGCTGAT CATTATGCAA AAGGAGTCAT TGCTAACAGA 
CGCAATGAGG TTCTGGACAG GTGGCGTCGT CTGAAGGCTC AGATGATTGA GAAGAGATCT 
AAGCTGGGAG AATCTCAGAC CCTCCAACAG TTCAGTCGTG ATGTTGATGA AATAGAAGCT 
TGGATCAGTG AAAAGCTTCA AACTGCAAGT GATGAGTCAT ATAAGGATCC CACAAACATC 
CAGCTTTCCA AACTGCTGAG CAAGCACCAG AAGCACCAAG CCTTTGAAGC TGAGCTCCAC 
GCCAACGCAG ATCGGATTCG TGGAGTCATT GAAATGGGGA ACTCTCTTAT TGAAAGAGGA 
GCGTGTGCTG GCAGCGAGGA TGCTGTGAAG GCTCGGCTGG CTGCCCTGGC TGACCAATGG 
GAGTTCCTGG TCCAGAAGTC ATCAGAGAAG AGTCAGAAAC TGAAAGAAGC AAATAAACAG 
CAGAATTTCA ATACCGGCAT CAAGGACTTT GATTTCTGGC TTTCAGAGGT GGAAGCTTTG 
TTGGCATCTG AAGACTATGG GAAGGACTTG GCATCAGTGA ACAACCTTCT GAAGAAGCAC 
CAATTACTGG AAGCTGATAT ATCTGCTCAT GAGGATCGGC TGAAGGACCT GAACAGCCAG 
GCTGACAGTT TGATGACCAG CAGTGCTTTC GATACCTCCC AAGTAAAGGA TAAACGTGAA 
ACTATAAATG GGCGCTTCCA GAGAATCAAG AGCATGGCAG CTGCCCGCCG TGCGAAGCTC 
AACGAGTCGC ACCGCTTGCA TCAGTTCTTC CGTGACATGG ATGATGAGGA GTCCTGGATC 
AAAGAGAAGA AACTGTTGGT GAGCTCAGAG GACTATGGCA GAGACCTGAC TGGTGTGCAG 
AACCTGAGGA AGAAACATAA GCGCTTGGAA GCAGAATTAG CTGCCCATGA ACCTGCTATC 
CAGGGTGTTC TAGACACTGG CAAGAAGCTT TCAGATGATA ACACAATTGG GAAGGAGGAG 
ATACAGCAAA GACTGGCTCA GTTTGTGGAC CACTGGAAAG AGTTAAAACA GCTGGCAGCT 
GCTCGGGGGC AGCGTCTGGA GGAGTCCCTG GAGTACCAAC AGTTTGTAGC AAATGTTGAG 
GAAGAGGAGG CATGGATCAA CGAGAAAATG ACATTGGTAG CCAGTGAGGA TTATGGAGAC 
ACACTTGCTG CTATCCAGGG CTTGCTGAAG AAGCATGAAG CATTCGAGAC TGACTTTACT 
GTCCACAAAG ACAGAGTGAA TGATGTTTGC GCTAATGGAG AGGATCTCAT TAAAAAGAAT 
AATCACCATG TGGAGAACAT TACTGCTAAG ATGAAGGGCC TCAAGGGCAA GGTATCAGAT 
CTGGAGAAAG CCGCAGCTCA GAGGAAAGCC AAACTGGATG AGAACTCTGC CTTCCTCCAG 
TTCAACTGGA AAGCAGATGT GGTGGAGTCA TGGATAGGTG AGAAGGAAAA CAGTCTGAAG 
ACAGATGATT ATGGCCGTGA CCTCTCCTCT GTGCAAACAC TACTCACCAA ACAGGAAACC 
TTTGATGCTG GACTTCAGGC TTTCCAGCAG GAGGGAATTG CAAACATCAC TGCTCTGAAA 
GACCAGCTAC TGGCAGCCAA ACATATCCAA TCAAAGGCCA TTGAGGTTCG TCACGCTTCC 
TTGATGAAAC GCTGGAATCA GCTGCTTGCT AATTCTGCAG CCAGGAAAAA GAAACTCTTG 
GAGGCTCAGG AGCACTTCAG AAAGGTTGAA GATCTCTTCT TGACTTTTGC CAAGAAGGCC 
TCTGCCTTCA ACAGTTGGTT TGAGAATGCT GAGGAGGACC TGACGGATCC CGTGCGCTGC 
AATTCCCTGG AAGAAATCAA AGCACTGCGA GAAGCCCACG ATGCGTTCCG TTCCTCACTT 
AGCTCTGCCC AAGCTGACTT CAACCAGCTG GCAGAGCTTG ATCGGCAGAT CAAGAGCTTC 
CGTGTAGCCT CCAACCCGTA CACTTGGTTT ACTATGGAGG CTCTTGAAGA AACTTGGAGG 
AATCTGCAGA AAATTATCAA GGAGCGTGAA TTGGAGTTGC AGAAGGAGCA GCGGAGGCAG 
GAAGAAAATG ACAAGTTGCG CCAGGAGTTT GCTCAGCATG CTAATGCCTT CCACCAGTGG 
ATTCAGGAGA CAAGGACTTA CCTGCTAGAT GGCATAGCCT ATCGTCGTGT CATTCGTGTC 
TATCAGTATG AAGTTGGGGA TGATCTATCT GGAAGGTCCT GCATGGTGGA GGAGTCGGGA 
ACACTGGAAT CCCAGCTGGA AGCTACTAAA CGCAAGCACC AGGAGATCCG GGCTATGAGG 
AGCCAGCTGA AGAAGATTGA GGACCTTGGA GCAGCCATGG AAGAGGCACT TATCTTGGAC 
AACAAATACA CAGAGCACAG CACCGTGGGG CTGGCGCAGC AGTGGGACCA GCTTGACCAG 
CTGGGAATGA GAATGCAGCA CAATCTGGAA CAGCAGATTC AAGCTCGAAA CACAACTGGA 
GTCACAGAGG AGGCCCTGAA GGAGTTCAGC ATGATGTTCA AGCACTTTGA CAAGGACAAA 
TCTGGACGTC TTAATCACCA GGAGTTTAAG TCTTGCTTGC GCTCCCTTGG CTATGACCTG 
CCCATGGTTG AGGAAGGAGA GCCTGACCCC GAATTTGAGT CTATTCTTGA CACTGTTGAT 
CCCAACAGGG ATGGCCACGT CTCGCTGCAG GAGTACATGG CGTTCATGAT CAGCAGGGAA 
ACAGAGAACG TGAAATCCAG CGAGGAGATT GAGAGTGCTT TCCGTGCCCT CAGCTCGGAG 
GGGAAGCCTT ACGTGACCAA GGAGGAGCTC TACCAGAACC TGACCCGGGA GCAGGCCGAC 
TACTGCATTT CTCACATGAA GCCCTACATG GATGGCAAGG GCAGAGAACT TCCTTCTGCC 
TACGACTACA TAGAATTTAC ACGTTCACTC TTTGTGAATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_015279283.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015134769.1 Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X2, mRNA.

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CloneID	OGa43309
Clone ID Related Accession (Same CDS sequence)	XM_015279283.1
Accession Version	XM_015279283.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7587bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG 
TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC 
TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT 
GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT 
GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG 
ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAT CTTTCACGCC TTACAGGACT 
GCACAGCGCC CAATAGTAAC AGCCACCCAA GTTGGGTCCC ACATACGAAC TCGTCTACAG 
GAGCTGCACC GACTATGGGA GTTACTGCTG GAAAAAATGA GAGAGAAGGG AGTGAAGCTG 
TTGCAAGCAC AGAAGCTGGT GCAATTCTTA CGGGAATGTG AAGATGTCAT GGACTGGATC 
AATGACAAGG AAGCAATAGT GACCTCAGAA GAGCTTGGAC AGGACTTAGA GCATGTTGAG 
GTTTTGCAGA AGAAATTTGA AGAGTTCCAA ACAGATCTGG CAGCTCATGA GGAGAGAGTG 
AATGAAGTGA ACCAGTTCGC TGGCAAACTT ATCCAGGAAC AGCACCCTGA GGAGGAACTT 
ATAAAGTCCA AACAGGATGA AGTAAATGCA AGCTGGCAGC GTCTTAAGGG GCTTGCCCAA 
CAGAGGCAAG GAAAACTCTT TGGGGCAGCT GAAGTTCAGC GCTTCAACAG GGATGTGGAT 
GAAACTATCA GCTGGATTAA GGAGAAAGGG CAGTTGATGG CCTCAGATGA CTTTGGCAGA 
GACTTGGCCA GCGTGCAAGC TTTACTACGT AAGCATGAAG GCCTGGAAAG AGATCTTGCA 
GCTTTGGAGG ATAAGGTTAA GGCCCTCTGT GCAGAAGCTG ACCGTTTGCA GCAGTCTCAC 
CCTATAAATG CTTCTCAAAT TCAAGTGAAA CGGGAGGAAC TGATTGCCAA CTGGGAGCAG 
ATCCGAACTC TGGCAGCAGA GAGGCATGCT CGCCTTAATG ACTCCTACAG GTTGCAGCGC 
TTTCTTGCAG ATTTCCGGGA CCTCACTAGC TGGGTAACTG AGATGAAGGC TCTGATAAAT 
GCTGATGAGC TTGCCAATGA TGTGGCTGGA GCAGAAGCCC TTCTAGATAG ACATCAGGAA 
CATAAGGGAG AAATTGATGC TCATGAGGAT AGCTTCAGAT CTGCTGATGA GTCTGGGCAG 
GCTTTGCTTG CAGCTGGGCA CTATGCTTCT GATGAAGTTA AAGAAAAGCT GACTATCCTC 
TCAGATGAAA GATCAGCCTT GCTGGAACTA TGGGAGCTTC GCAGGCAACA GTATGAGCAG 
TGCATGGATC TGCAGCTTTT CTACAGAGAT ACTGAACAAG TTGACAACTG GATGAGCAAA 
CAAGAAGCTT TTCTGCTGAA CGAAGACCTT GGTGATTCTC TGGATAGCGT GGAGGCTCTT 
CTAAAGAAGC ATGAAGATTT TGAGAAATCC CTAAGTGCTC AAGAGGAGAA GATCACAGCA 
CTGGATGAGT TTGCTACTAA ACTGATTCAA AATAACCATT ATGCCATGGA TGACGTTGCT 
ACGCGCAGAG ATGCTCTTCT GAGTCGCCGA AATGCTCTTC ATGAAAGAGC CATGAAGCGC 
CGTGCCCAAC TGGCAGACTC TTTCCATCTC CAGCAGTTTT TCAGGGACTC TGATGAACTG 
AAGAGTTGGG TTAATGAAAA AATGAAAACT GCTACCGATG AGGCTTACAA GGATCCATCC 
AACTTGCAAG GTAAAGTTCA GAAGCATCAG GCTTTTGAAG CAGAGCTTTC TGCTAATCAG 
AGTCGTATTG ATGCACTGGA GAAAGCTGGC CAGAAGTTGA TTGATGTCAA TCACTATGCA 
TCTGATGAGG TGGCAGCTCG CATGAATGAA GTCATCAGCT TGTGGAAGAA ACTTCTGGAA 
GCCACCGAGC TCAAAGGTAT AAAGCTGCGT GAAGCCAATC AACAGCAGCA ATTTAATCGC 
AATGTAGAAG ACATTGAGTT GTGGCTGTAT GAAGTGGAGG GTCACTTGGC TTCTGATGAT 
TATGGAAAAG ATCTTACCAG CGTTCAGAAT CTTCAGAAGA AACATGCCCT GCTAGAGGCA 
GATGTTGCTG CCCATCAGGA TCGGATAGAT GGCATTACCA TCCAGGCACG CCAGTTCCAA 
GATGCTGGGC ACTTTGATGC TGACAATATC AAGAAGAAAC AAGAAGCTTT AGTAGCTCGT 
TATGAAGCTC TGAAGGATCC TATGGTGGCT CGCAAGCAGA AACTTGCAGA TTCTCTTCGC 
CTGCAGCAGC TTTTCCGTGA CATCGAGGAT GAAGAGACCT GGATCAGGGA AAAGGAACCT 
ATTGCAGCCT CAACAAACCG AGGCAAGGAC TTAATTGGTG TCCAGAATCT GCTAAAGAAG 
CACCAGGCTT TGCAGGCAGA AATTGCAGGC CATGAGCCTC GCATTAAAGC AGTCACACAA 
AAAGGAAATG CGATGGTGGA AGAGGGACAT TTTGCTGCTG AAGATGTGAA GATCAAATTG 
AATGAACTAA ACCAAAAATG GGACTCTCTG AAAGCCAAGG CATCTCAACG GCGACAGGAT 
CTAGAGGATT CTCTGCAAGC TCAGCAGTAT TTTGCTGATG CTAATGAGGC AGAATCATGG 
ATGAGGGAAA AGGAGCCCAT TGTAGGCAGC ACGGATTATG GAAAGGATGA AGACTCTGCT 
GAGGCTCTCC TGAAGAAGCA TGAAGCTTTG ATGTCTGATC TTTCTGCTTA CGGCAGTAGC 
ATACAGGCAT TAAGGGAACA GGCCCAGTCC TGCAGGCAAC AAGTTGCTCC CACTGATGAT 
GAAACTGGAA AAGAGCTTGT GCTAGCACTC TATGATTACC AAGAGAAGAG TCCTCGGGAG 
GTGACTATGA AGAAAGGAGA TATTCTAACC CTGCTCAACA GCACCAACAA GGACTGGTGG 
AAGGTTGAAG TTAACGATCG TCAGGGCTTT GTACCAGCTG CCTATGTGAA AAAACTAGAT 
CCTGCCCAGT CTGCATCCCG AGAGAATCTT CTAGAAGAGC AAGGCAGCAT AGCATTGCGA 
CAGGAGCAAA TTGACAACCA GACTCTCATT ACTAAGGAAG TCGGCAGTGT ATCTCTGCGT 
ATGAAACAGG TCGAAGAACT GTATCATTCC CTTCTTGAGC TGGGAGAAAA ACGTAAAGGC 
ATGCTAGAAA AAAGCTGCAA GAAGTTTATG CTTTTCCGTG AGGCTAATGA GCTTCAACAG 
TGGATCAATG AGAAGGAAGC TGCGCTCACT AATGAGGAAG TGGGTGCTGA TTTGGAGCAG 
GTGGAGGTGC TACAGAAGAA GTTTGATGAT TTTCAGAAGG ATTTAAAAGC TAATGAGTCA 
CGACTGAAGG ACATAAACAA GGTTGCAAAT GACCTGGAGT CTGAAGGGCT GATGGCAGAG 
GAAGTGCAAG CAGTAGAGCA CCAGGAAGTC TATGGAATGA TGCCCAGAGA TGAAACTGAT 
TCTAAGACAG CCTCTCCTTG GAAGTCTGCA CGTATGATGG TACACACAGT GGCAACGTTT 
AACTCAATCA AGGAACTGAA TGAACGCTGG AGATCCCTGC AGCAGTTGGC AGAGGAGCGA 
AGCCAGCTGT TGGGCAGCGC TCATGAAGTC CAGAGGTTCC ACAGAGATGC TGATGAAACA 
AAAGAATGGA TAGAAGAGAA GAATCAAGCA TTAAATACCG ACAACTATGG ACATGACTTG 
GCCAGTGTGC AGGCTCTGCA GCGCAAGCAT GAAGGCTTTG AGAGAGACTT GGCAGCTCTT 
GGAGACAAGG TGAATTCTCT TGGTGAAACT GCCCAGCGTC TGATCCAGTC ACATCCAGAA 
TCAGCTGAAG ATCTTCAAGA AAAATGTACT GAGTTGAACC AAGCCTGGAG TAGTCTGGGA 
AAACGTGCTG ACCAACGCAA AGAGAAGCTT GGAGATTCTC ATGACCTGCA GCGTTTCCTC 
AGTGATTTTA GAGACCTTAT GTCTTGGATC AATGGAATCC GGGGTCTGGT CTCCTCAGAT 
GAACTTGCAA AAGATGTGAC TGGAGCTGAA GCTCTGTTGG AAAGGCATCA GGAGCACCGT 
ACTGAAATAG ATGCACGGGC TGGCACTTTT CAGGCATTTG AACAGTTTGG ACAGCAGCTT 
CTGGCTCGTG GACACTATGC CAGCCCAGAG ATTAAGGAGA AACTGGATAT TCTAGATCAA 
GAACGGACAG ACCTAGAGAA GGCCTGGGTC CAGCGCAGAA TGATGCTAGA CCAGTGCTTA 
GAACTACAGC TGTTTCATCG GGATTGTGAA CAAGCTGAAA ACTGGATGGC TGCCCGAGAG 
GCGTTCCTAA ATACAGAAGA CAAAGGAGAC TCCTTAGACA GCGTGGAGGC ACTCATCAAG 
AAGCATGAAG ATTTCGATAA AGCAATCAAT GTCCAGGAAG AGAAAATTGC TGTCTTGCAG 
TCTTTTGCTG ACCAACTGAT TGCTGCTGAT CATTATGCAA AAGGAGTCAT TGCTAACAGA 
CGCAATGAGG TTCTGGACAG GTGGCGTCGT CTGAAGGCTC AGATGATTGA GAAGAGATCT 
AAGCTGGGAG AATCTCAGAC CCTCCAACAG TTCAGTCGTG ATGTTGATGA AATAGAAGCT 
TGGATCAGTG AAAAGCTTCA AACTGCAAGT GATGAGTCAT ATAAGGATCC CACAAACATC 
CAGAGCAAGC ACCAGAAGCA CCAAGCCTTT GAAGCTGAGC TCCACGCCAA CGCAGATCGG 
ATTCGTGGAG TCATTGAAAT GGGGAACTCT CTTATTGAAA GAGGAGCGTG TGCTGGCAGC 
GAGGATGCTG TGAAGGCTCG GCTGGCTGCC CTGGCTGACC AATGGGAGTT CCTGGTCCAG 
AAGTCATCAG AGAAGAGTCA GAAACTGAAA GAAGCAAATA AACAGCAGAA TTTCAATACC 
GGCATCAAGG ACTTTGATTT CTGGCTTTCA GAGGTGGAAG CTTTGTTGGC ATCTGAAGAC 
TATGGGAAGG ACTTGGCATC AGTGAACAAC CTTCTGAAGA AGCACCAATT ACTGGAAGCT 
GATATATCTG CTCATGAGGA TCGGCTGAAG GACCTGAACA GCCAGGCTGA CAGTTTGATG 
ACCAGCAGTG CTTTCGATAC CTCCCAAGTA AAGGATAAAC GTGAAACTAT AAATGGGCGC 
TTCCAGAGAA TCAAGAGCAT GGCAGCTGCC CGCCGTGCGA AGCTCAACGA GTCGCACCGC 
TTGCATCAGT TCTTCCGTGA CATGGATGAT GAGGAGTCCT GGATCAAAGA GAAGAAACTG 
TTGGTGAGCT CAGAGGACTA TGGCAGAGAC CTGACTGGTG TGCAGAACCT GAGGAAGAAA 
CATAAGCGCT TGGAAGCAGA ATTAGCTGCC CATGAACCTG CTATCCAGGG TGTTCTAGAC 
ACTGGCAAGA AGCTTTCAGA TGATAACACA ATTGGGAAGG AGGAGATACA GCAAAGACTG 
GCTCAGTTTG TGGACCACTG GAAAGAGTTA AAACAGCTGG CAGCTGCTCG GGGGCAGCGT 
CTGGAGGAGT CCCTGGAGTA CCAACAGTTT GTAGCAAATG TTGAGGAAGA GGAGGCATGG 
ATCAACGAGA AAATGACATT GGTAGCCAGT GAGGATTATG GAGACACACT TGCTGCTATC 
CAGGGCTTGC TGAAGAAGCA TGAAGCATTC GAGACTGACT TTACTGTCCA CAAAGACAGA 
GTGAATGATG TTTGCGCTAA TGGAGAGGAT CTCATTAAAA AGAATAATCA CCATGTGGAG 
AACATTACTG CTAAGATGAA GGGCCTCAAG GGCAAGGTAT CAGATCTGGA GAAAGCCGCA 
GCTCAGAGGA AAGCCAAACT GGATGAGAAC TCTGCCTTCC TCCAGTTCAA CTGGAAAGCA 
GATGTGGTGG AGTCATGGAT AGGTGAGAAG GAAAACAGTC TGAAGACAGA TGATTATGGC 
CGTGACCTCT CCTCTGTGCA AACACTACTC ACCAAACAGG AAACCTTTGA TGCTGGACTT 
CAGGCTTTCC AGCAGGAGGG AATTGCAAAC ATCACTGCTC TGAAAGACCA GCTACTGGCA 
GCCAAACATA TCCAATCAAA GGCCATTGAG GTTCGTCACG CTTCCTTGAT GAAACGCTGG 
AATCAGCTGC TTGCTAATTC TGCAGCCAGG AAAAAGAAAC TCTTGGAGGC TCAGGAGCAC 
TTCAGAAAGG TTGAAGATCT CTTCTTGACT TTTGCCAAGA AGGCCTCTGC CTTCAACAGT 
TGGTTTGAGA ATGCTGAGGA GGACCTGACG GATCCCGTGC GCTGCAATTC CCTGGAAGAA 
ATCAAAGCAC TGCGAGAAGC CCACGATGCG TTCCGTTCCT CACTTAGCTC TGCCCAAGCT 
GACTTCAACC AGCTGGCAGA GCTTGATCGG CAGATCAAGA GCTTCCGTGT AGCCTCCAAC 
CCGTACACTT GGTTTACTAT GGAGGCTCTT GAAGAAACTT GGAGGAATCT GCAGAAAATT 
ATCAAGGAGC GTGAATTGGA GTTGCAGAAG GAGCAGCGGA GGCAGGAAGA AAATGACAAG 
TTGCGCCAGG AGTTTGCTCA GCATGCTAAT GCCTTCCACC AGTGGATTCA GGAGACAAGG 
ACTTACCTGC TAGATGGCAT AGCCTATCGT CGTGTCATTC GTGTCTATCA GTATGAAGTT 
GGGGATGATC TATCTGGAAG GTCCTGCATG GTGGAGGAGT CGGGAACACT GGAATCCCAG 
CTGGAAGCTA CTAAACGCAA GCACCAGGAG ATCCGGGCTA TGAGGAGCCA GCTGAAGAAG 
ATTGAGGACC TTGGAGCAGC CATGGAAGAG GCACTTATCT TGGACAACAA ATACACAGAG 
CACAGCACCG TGGGGCTGGC GCAGCAGTGG GACCAGCTTG ACCAGCTGGG AATGAGAATG 
CAGCACAATC TGGAACAGCA GATTCAAGCT CGAAACACAA CTGGAGTCAC AGAGGAGGCC 
CTGAAGGAGT TCAGCATGAT GTTCAAGCAC TTTGACAAGG ACAAATCTGG ACGTCTTAAT 
CACCAGGAGT TTAAGTCTTG CTTGCGCTCC CTTGGCTATG ACCTGCCCAT GGTTGAGGAA 
GGAGAGCCTG ACCCCGAATT TGAGTCTATT CTTGACACTG TTGATCCCAA CAGGGATGGC 
CACGTCTCGC TGCAGGAGTA CATGGCGTTC ATGATCAGCA GGGAAACAGA GAACGTGAAA 
TCCAGCGAGG AGATTGAGAG TGCTTTCCGT GCCCTCAGCT CGGAGGGGAA GCCTTACGTG 
ACCAAGGAGG AGCTCTACCA GAACCTGACC CGGGAGCAGG CCGACTACTG CATTTCTCAC 
ATGAAGCCCT ACATGGATGG CAAGGGCAGA GAACTTCCTT CTGCCTACGA CTACATAGAA 
TTTACACGTT CACTCTTTGT GAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015134769.1
CDS	18..7604
Translation	MLSSFRKVKVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSSLRRQKLEDSYRFQFFQRDADELEKWIQEKLQIASDENYKDPSNLQGKLQKHQAFEAEVQANSGAIVKLDETGNQMINEGHFASETIRSFTPYRTAQRPIVTATQVGSHIRTRLQELHRLWELLLEKMREKGVKLLQAQKLVQFLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDLAAHEERVNEVNQFAGKLIQEQHPEEELIKSKQDEVNASWQRLKGLAQQRQGKLFGAAEVQRFNRDVDETISWIKEKGQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPINASQIQVKREELIANWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFRSADESGQALLAAGHYASDEVKEKLTILSDERSALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMDDVATRRDALLSRRNALHERAMKRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYASDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTSVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDADNIKKKQEALVARYEALKDPMVARKQKLADSLRLQQLFRDIEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKIKLNELNQKWDSLKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPTDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTLITKEVGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTNEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVANDLESEGLMAEEVQAVEHQEVYGMMPRDETDSKTASPWKSARMMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAQRLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKEKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLARGHYASPEIKEKLDILDQERTDLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAVLQSFADQLIAADHYAKGVIANRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQSKHQKHQAFEAELHANADRIRGVIEMGNSLIERGACAGSEDAVKARLAALADQWEFLVQKSSEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRETINGRFQRIKSMAAARRAKLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVDHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCANGEDLIKKNNHHVENITAKMKGLKGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHIQSKAIEVRHASLMKRWNQLLANSAARKKKLLEAQEHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGIAYRRVIRVYQYEVGDDLSGRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFESILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCISHMKPYMDGKGRELPSAYDYIEFTRSLFVN

Target ORF information:

RefSeq Version	XM_015279283.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279283.1

ORF Insert Sequence:

ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG 
TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC 
TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT 
GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT 
GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG 
ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAT CTTTCACGCC TTACAGGACT 
GCACAGCGCC CAATAGTAAC AGCCACCCAA GTTGGGTCCC ACATACGAAC TCGTCTACAG 
GAGCTGCACC GACTATGGGA GTTACTGCTG GAAAAAATGA GAGAGAAGGG AGTGAAGCTG 
TTGCAAGCAC AGAAGCTGGT GCAATTCTTA CGGGAATGTG AAGATGTCAT GGACTGGATC 
AATGACAAGG AAGCAATAGT GACCTCAGAA GAGCTTGGAC AGGACTTAGA GCATGTTGAG 
GTTTTGCAGA AGAAATTTGA AGAGTTCCAA ACAGATCTGG CAGCTCATGA GGAGAGAGTG 
AATGAAGTGA ACCAGTTCGC TGGCAAACTT ATCCAGGAAC AGCACCCTGA GGAGGAACTT 
ATAAAGTCCA AACAGGATGA AGTAAATGCA AGCTGGCAGC GTCTTAAGGG GCTTGCCCAA 
CAGAGGCAAG GAAAACTCTT TGGGGCAGCT GAAGTTCAGC GCTTCAACAG GGATGTGGAT 
GAAACTATCA GCTGGATTAA GGAGAAAGGG CAGTTGATGG CCTCAGATGA CTTTGGCAGA 
GACTTGGCCA GCGTGCAAGC TTTACTACGT AAGCATGAAG GCCTGGAAAG AGATCTTGCA 
GCTTTGGAGG ATAAGGTTAA GGCCCTCTGT GCAGAAGCTG ACCGTTTGCA GCAGTCTCAC 
CCTATAAATG CTTCTCAAAT TCAAGTGAAA CGGGAGGAAC TGATTGCCAA CTGGGAGCAG 
ATCCGAACTC TGGCAGCAGA GAGGCATGCT CGCCTTAATG ACTCCTACAG GTTGCAGCGC 
TTTCTTGCAG ATTTCCGGGA CCTCACTAGC TGGGTAACTG AGATGAAGGC TCTGATAAAT 
GCTGATGAGC TTGCCAATGA TGTGGCTGGA GCAGAAGCCC TTCTAGATAG ACATCAGGAA 
CATAAGGGAG AAATTGATGC TCATGAGGAT AGCTTCAGAT CTGCTGATGA GTCTGGGCAG 
GCTTTGCTTG CAGCTGGGCA CTATGCTTCT GATGAAGTTA AAGAAAAGCT GACTATCCTC 
TCAGATGAAA GATCAGCCTT GCTGGAACTA TGGGAGCTTC GCAGGCAACA GTATGAGCAG 
TGCATGGATC TGCAGCTTTT CTACAGAGAT ACTGAACAAG TTGACAACTG GATGAGCAAA 
CAAGAAGCTT TTCTGCTGAA CGAAGACCTT GGTGATTCTC TGGATAGCGT GGAGGCTCTT 
CTAAAGAAGC ATGAAGATTT TGAGAAATCC CTAAGTGCTC AAGAGGAGAA GATCACAGCA 
CTGGATGAGT TTGCTACTAA ACTGATTCAA AATAACCATT ATGCCATGGA TGACGTTGCT 
ACGCGCAGAG ATGCTCTTCT GAGTCGCCGA AATGCTCTTC ATGAAAGAGC CATGAAGCGC 
CGTGCCCAAC TGGCAGACTC TTTCCATCTC CAGCAGTTTT TCAGGGACTC TGATGAACTG 
AAGAGTTGGG TTAATGAAAA AATGAAAACT GCTACCGATG AGGCTTACAA GGATCCATCC 
AACTTGCAAG GTAAAGTTCA GAAGCATCAG GCTTTTGAAG CAGAGCTTTC TGCTAATCAG 
AGTCGTATTG ATGCACTGGA GAAAGCTGGC CAGAAGTTGA TTGATGTCAA TCACTATGCA 
TCTGATGAGG TGGCAGCTCG CATGAATGAA GTCATCAGCT TGTGGAAGAA ACTTCTGGAA 
GCCACCGAGC TCAAAGGTAT AAAGCTGCGT GAAGCCAATC AACAGCAGCA ATTTAATCGC 
AATGTAGAAG ACATTGAGTT GTGGCTGTAT GAAGTGGAGG GTCACTTGGC TTCTGATGAT 
TATGGAAAAG ATCTTACCAG CGTTCAGAAT CTTCAGAAGA AACATGCCCT GCTAGAGGCA 
GATGTTGCTG CCCATCAGGA TCGGATAGAT GGCATTACCA TCCAGGCACG CCAGTTCCAA 
GATGCTGGGC ACTTTGATGC TGACAATATC AAGAAGAAAC AAGAAGCTTT AGTAGCTCGT 
TATGAAGCTC TGAAGGATCC TATGGTGGCT CGCAAGCAGA AACTTGCAGA TTCTCTTCGC 
CTGCAGCAGC TTTTCCGTGA CATCGAGGAT GAAGAGACCT GGATCAGGGA AAAGGAACCT 
ATTGCAGCCT CAACAAACCG AGGCAAGGAC TTAATTGGTG TCCAGAATCT GCTAAAGAAG 
CACCAGGCTT TGCAGGCAGA AATTGCAGGC CATGAGCCTC GCATTAAAGC AGTCACACAA 
AAAGGAAATG CGATGGTGGA AGAGGGACAT TTTGCTGCTG AAGATGTGAA GATCAAATTG 
AATGAACTAA ACCAAAAATG GGACTCTCTG AAAGCCAAGG CATCTCAACG GCGACAGGAT 
CTAGAGGATT CTCTGCAAGC TCAGCAGTAT TTTGCTGATG CTAATGAGGC AGAATCATGG 
ATGAGGGAAA AGGAGCCCAT TGTAGGCAGC ACGGATTATG GAAAGGATGA AGACTCTGCT 
GAGGCTCTCC TGAAGAAGCA TGAAGCTTTG ATGTCTGATC TTTCTGCTTA CGGCAGTAGC 
ATACAGGCAT TAAGGGAACA GGCCCAGTCC TGCAGGCAAC AAGTTGCTCC CACTGATGAT 
GAAACTGGAA AAGAGCTTGT GCTAGCACTC TATGATTACC AAGAGAAGAG TCCTCGGGAG 
GTGACTATGA AGAAAGGAGA TATTCTAACC CTGCTCAACA GCACCAACAA GGACTGGTGG 
AAGGTTGAAG TTAACGATCG TCAGGGCTTT GTACCAGCTG CCTATGTGAA AAAACTAGAT 
CCTGCCCAGT CTGCATCCCG AGAGAATCTT CTAGAAGAGC AAGGCAGCAT AGCATTGCGA 
CAGGAGCAAA TTGACAACCA GACTCTCATT ACTAAGGAAG TCGGCAGTGT ATCTCTGCGT 
ATGAAACAGG TCGAAGAACT GTATCATTCC CTTCTTGAGC TGGGAGAAAA ACGTAAAGGC 
ATGCTAGAAA AAAGCTGCAA GAAGTTTATG CTTTTCCGTG AGGCTAATGA GCTTCAACAG 
TGGATCAATG AGAAGGAAGC TGCGCTCACT AATGAGGAAG TGGGTGCTGA TTTGGAGCAG 
GTGGAGGTGC TACAGAAGAA GTTTGATGAT TTTCAGAAGG ATTTAAAAGC TAATGAGTCA 
CGACTGAAGG ACATAAACAA GGTTGCAAAT GACCTGGAGT CTGAAGGGCT GATGGCAGAG 
GAAGTGCAAG CAGTAGAGCA CCAGGAAGTC TATGGAATGA TGCCCAGAGA TGAAACTGAT 
TCTAAGACAG CCTCTCCTTG GAAGTCTGCA CGTATGATGG TACACACAGT GGCAACGTTT 
AACTCAATCA AGGAACTGAA TGAACGCTGG AGATCCCTGC AGCAGTTGGC AGAGGAGCGA 
AGCCAGCTGT TGGGCAGCGC TCATGAAGTC CAGAGGTTCC ACAGAGATGC TGATGAAACA 
AAAGAATGGA TAGAAGAGAA GAATCAAGCA TTAAATACCG ACAACTATGG ACATGACTTG 
GCCAGTGTGC AGGCTCTGCA GCGCAAGCAT GAAGGCTTTG AGAGAGACTT GGCAGCTCTT 
GGAGACAAGG TGAATTCTCT TGGTGAAACT GCCCAGCGTC TGATCCAGTC ACATCCAGAA 
TCAGCTGAAG ATCTTCAAGA AAAATGTACT GAGTTGAACC AAGCCTGGAG TAGTCTGGGA 
AAACGTGCTG ACCAACGCAA AGAGAAGCTT GGAGATTCTC ATGACCTGCA GCGTTTCCTC 
AGTGATTTTA GAGACCTTAT GTCTTGGATC AATGGAATCC GGGGTCTGGT CTCCTCAGAT 
GAACTTGCAA AAGATGTGAC TGGAGCTGAA GCTCTGTTGG AAAGGCATCA GGAGCACCGT 
ACTGAAATAG ATGCACGGGC TGGCACTTTT CAGGCATTTG AACAGTTTGG ACAGCAGCTT 
CTGGCTCGTG GACACTATGC CAGCCCAGAG ATTAAGGAGA AACTGGATAT TCTAGATCAA 
GAACGGACAG ACCTAGAGAA GGCCTGGGTC CAGCGCAGAA TGATGCTAGA CCAGTGCTTA 
GAACTACAGC TGTTTCATCG GGATTGTGAA CAAGCTGAAA ACTGGATGGC TGCCCGAGAG 
GCGTTCCTAA ATACAGAAGA CAAAGGAGAC TCCTTAGACA GCGTGGAGGC ACTCATCAAG 
AAGCATGAAG ATTTCGATAA AGCAATCAAT GTCCAGGAAG AGAAAATTGC TGTCTTGCAG 
TCTTTTGCTG ACCAACTGAT TGCTGCTGAT CATTATGCAA AAGGAGTCAT TGCTAACAGA 
CGCAATGAGG TTCTGGACAG GTGGCGTCGT CTGAAGGCTC AGATGATTGA GAAGAGATCT 
AAGCTGGGAG AATCTCAGAC CCTCCAACAG TTCAGTCGTG ATGTTGATGA AATAGAAGCT 
TGGATCAGTG AAAAGCTTCA AACTGCAAGT GATGAGTCAT ATAAGGATCC CACAAACATC 
CAGAGCAAGC ACCAGAAGCA CCAAGCCTTT GAAGCTGAGC TCCACGCCAA CGCAGATCGG 
ATTCGTGGAG TCATTGAAAT GGGGAACTCT CTTATTGAAA GAGGAGCGTG TGCTGGCAGC 
GAGGATGCTG TGAAGGCTCG GCTGGCTGCC CTGGCTGACC AATGGGAGTT CCTGGTCCAG 
AAGTCATCAG AGAAGAGTCA GAAACTGAAA GAAGCAAATA AACAGCAGAA TTTCAATACC 
GGCATCAAGG ACTTTGATTT CTGGCTTTCA GAGGTGGAAG CTTTGTTGGC ATCTGAAGAC 
TATGGGAAGG ACTTGGCATC AGTGAACAAC CTTCTGAAGA AGCACCAATT ACTGGAAGCT 
GATATATCTG CTCATGAGGA TCGGCTGAAG GACCTGAACA GCCAGGCTGA CAGTTTGATG 
ACCAGCAGTG CTTTCGATAC CTCCCAAGTA AAGGATAAAC GTGAAACTAT AAATGGGCGC 
TTCCAGAGAA TCAAGAGCAT GGCAGCTGCC CGCCGTGCGA AGCTCAACGA GTCGCACCGC 
TTGCATCAGT TCTTCCGTGA CATGGATGAT GAGGAGTCCT GGATCAAAGA GAAGAAACTG 
TTGGTGAGCT CAGAGGACTA TGGCAGAGAC CTGACTGGTG TGCAGAACCT GAGGAAGAAA 
CATAAGCGCT TGGAAGCAGA ATTAGCTGCC CATGAACCTG CTATCCAGGG TGTTCTAGAC 
ACTGGCAAGA AGCTTTCAGA TGATAACACA ATTGGGAAGG AGGAGATACA GCAAAGACTG 
GCTCAGTTTG TGGACCACTG GAAAGAGTTA AAACAGCTGG CAGCTGCTCG GGGGCAGCGT 
CTGGAGGAGT CCCTGGAGTA CCAACAGTTT GTAGCAAATG TTGAGGAAGA GGAGGCATGG 
ATCAACGAGA AAATGACATT GGTAGCCAGT GAGGATTATG GAGACACACT TGCTGCTATC 
CAGGGCTTGC TGAAGAAGCA TGAAGCATTC GAGACTGACT TTACTGTCCA CAAAGACAGA 
GTGAATGATG TTTGCGCTAA TGGAGAGGAT CTCATTAAAA AGAATAATCA CCATGTGGAG 
AACATTACTG CTAAGATGAA GGGCCTCAAG GGCAAGGTAT CAGATCTGGA GAAAGCCGCA 
GCTCAGAGGA AAGCCAAACT GGATGAGAAC TCTGCCTTCC TCCAGTTCAA CTGGAAAGCA 
GATGTGGTGG AGTCATGGAT AGGTGAGAAG GAAAACAGTC TGAAGACAGA TGATTATGGC 
CGTGACCTCT CCTCTGTGCA AACACTACTC ACCAAACAGG AAACCTTTGA TGCTGGACTT 
CAGGCTTTCC AGCAGGAGGG AATTGCAAAC ATCACTGCTC TGAAAGACCA GCTACTGGCA 
GCCAAACATA TCCAATCAAA GGCCATTGAG GTTCGTCACG CTTCCTTGAT GAAACGCTGG 
AATCAGCTGC TTGCTAATTC TGCAGCCAGG AAAAAGAAAC TCTTGGAGGC TCAGGAGCAC 
TTCAGAAAGG TTGAAGATCT CTTCTTGACT TTTGCCAAGA AGGCCTCTGC CTTCAACAGT 
TGGTTTGAGA ATGCTGAGGA GGACCTGACG GATCCCGTGC GCTGCAATTC CCTGGAAGAA 
ATCAAAGCAC TGCGAGAAGC CCACGATGCG TTCCGTTCCT CACTTAGCTC TGCCCAAGCT 
GACTTCAACC AGCTGGCAGA GCTTGATCGG CAGATCAAGA GCTTCCGTGT AGCCTCCAAC 
CCGTACACTT GGTTTACTAT GGAGGCTCTT GAAGAAACTT GGAGGAATCT GCAGAAAATT 
ATCAAGGAGC GTGAATTGGA GTTGCAGAAG GAGCAGCGGA GGCAGGAAGA AAATGACAAG 
TTGCGCCAGG AGTTTGCTCA GCATGCTAAT GCCTTCCACC AGTGGATTCA GGAGACAAGG 
ACTTACCTGC TAGATGGCAT AGCCTATCGT CGTGTCATTC GTGTCTATCA GTATGAAGTT 
GGGGATGATC TATCTGGAAG GTCCTGCATG GTGGAGGAGT CGGGAACACT GGAATCCCAG 
CTGGAAGCTA CTAAACGCAA GCACCAGGAG ATCCGGGCTA TGAGGAGCCA GCTGAAGAAG 
ATTGAGGACC TTGGAGCAGC CATGGAAGAG GCACTTATCT TGGACAACAA ATACACAGAG 
CACAGCACCG TGGGGCTGGC GCAGCAGTGG GACCAGCTTG ACCAGCTGGG AATGAGAATG 
CAGCACAATC TGGAACAGCA GATTCAAGCT CGAAACACAA CTGGAGTCAC AGAGGAGGCC 
CTGAAGGAGT TCAGCATGAT GTTCAAGCAC TTTGACAAGG ACAAATCTGG ACGTCTTAAT 
CACCAGGAGT TTAAGTCTTG CTTGCGCTCC CTTGGCTATG ACCTGCCCAT GGTTGAGGAA 
GGAGAGCCTG ACCCCGAATT TGAGTCTATT CTTGACACTG TTGATCCCAA CAGGGATGGC 
CACGTCTCGC TGCAGGAGTA CATGGCGTTC ATGATCAGCA GGGAAACAGA GAACGTGAAA 
TCCAGCGAGG AGATTGAGAG TGCTTTCCGT GCCCTCAGCT CGGAGGGGAA GCCTTACGTG 
ACCAAGGAGG AGCTCTACCA GAACCTGACC CGGGAGCAGG CCGACTACTG CATTTCTCAC 
ATGAAGCCCT ACATGGATGG CAAGGGCAGA GAACTTCCTT CTGCCTACGA CTACATAGAA 
TTTACACGTT CACTCTTTGT GAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_015279284.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015134770.1 Gallus gallus spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X3, mRNA.

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CloneID	OGa43310
Clone ID Related Accession (Same CDS sequence)	XM_015279284.1 , XM_015279284.2
Accession Version	XM_015279284.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7566bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCAA GTGGGGTAAA AGTGTTGGAA ACAGCAGAAG ATATCCAAGA ACGGCGTCAG 
CAGGTTTTGG ACCGTTACCA CAGGTTCAAG GAGCTGTCTT CTCTGAGGCG CCAAAAACTC 
GAAGATTCCT ATCGGTTCCA GTTCTTCCAG CGCGATGCAG ATGAGCTGGA AAAATGGATC 
CAAGAGAAAC TCCAGATAGC ATCTGATGAA AATTACAAAG ACCCAAGCAA TTTACAGGGG 
AAGCTGCAGA AGCACCAGGC CTTTGAAGCT GAGGTGCAGG CCAATTCAGG GGCTATCGTT 
AAGCTGGATG AGACTGGAAA TCAGATGATC AATGAAGGCC ATTTTGCATC TGAAACCATA 
AGATCTTTCA CGCCTTACAG GACTGCACAG CGCCCAATAG TAACAGCCAC CCAAGTTGGG 
TCCCACATAC GAACTCGTCT ACAGGAGCTG CACCGACTAT GGGAGTTACT GCTGGAAAAA 
ATGAGAGAGA AGGGAGTGAA GCTGTTGCAA GCACAGAAGC TGGTGCAATT CTTACGGGAA 
TGTGAAGATG TCATGGACTG GATCAATGAC AAGGAAGCAA TAGTGACCTC AGAAGAGCTT 
GGACAGGACT TAGAGCATGT TGAGGTTTTG CAGAAGAAAT TTGAAGAGTT CCAAACAGAT 
CTGGCAGCTC ATGAGGAGAG AGTGAATGAA GTGAACCAGT TCGCTGGCAA ACTTATCCAG 
GAACAGCACC CTGAGGAGGA ACTTATAAAG TCCAAACAGG ATGAAGTAAA TGCAAGCTGG 
CAGCGTCTTA AGGGGCTTGC CCAACAGAGG CAAGGAAAAC TCTTTGGGGC AGCTGAAGTT 
CAGCGCTTCA ACAGGGATGT GGATGAAACT ATCAGCTGGA TTAAGGAGAA AGGGCAGTTG 
ATGGCCTCAG ATGACTTTGG CAGAGACTTG GCCAGCGTGC AAGCTTTACT ACGTAAGCAT 
GAAGGCCTGG AAAGAGATCT TGCAGCTTTG GAGGATAAGG TTAAGGCCCT CTGTGCAGAA 
GCTGACCGTT TGCAGCAGTC TCACCCTATA AATGCTTCTC AAATTCAAGT GAAACGGGAG 
GAACTGATTG CCAACTGGGA GCAGATCCGA ACTCTGGCAG CAGAGAGGCA TGCTCGCCTT 
AATGACTCCT ACAGGTTGCA GCGCTTTCTT GCAGATTTCC GGGACCTCAC TAGCTGGGTA 
ACTGAGATGA AGGCTCTGAT AAATGCTGAT GAGCTTGCCA ATGATGTGGC TGGAGCAGAA 
GCCCTTCTAG ATAGACATCA GGAACATAAG GGAGAAATTG ATGCTCATGA GGATAGCTTC 
AGATCTGCTG ATGAGTCTGG GCAGGCTTTG CTTGCAGCTG GGCACTATGC TTCTGATGAA 
GTTAAAGAAA AGCTGACTAT CCTCTCAGAT GAAAGATCAG CCTTGCTGGA ACTATGGGAG 
CTTCGCAGGC AACAGTATGA GCAGTGCATG GATCTGCAGC TTTTCTACAG AGATACTGAA 
CAAGTTGACA ACTGGATGAG CAAACAAGAA GCTTTTCTGC TGAACGAAGA CCTTGGTGAT 
TCTCTGGATA GCGTGGAGGC TCTTCTAAAG AAGCATGAAG ATTTTGAGAA ATCCCTAAGT 
GCTCAAGAGG AGAAGATCAC AGCACTGGAT GAGTTTGCTA CTAAACTGAT TCAAAATAAC 
CATTATGCCA TGGATGACGT TGCTACGCGC AGAGATGCTC TTCTGAGTCG CCGAAATGCT 
CTTCATGAAA GAGCCATGAA GCGCCGTGCC CAACTGGCAG ACTCTTTCCA TCTCCAGCAG 
TTTTTCAGGG ACTCTGATGA ACTGAAGAGT TGGGTTAATG AAAAAATGAA AACTGCTACC 
GATGAGGCTT ACAAGGATCC ATCCAACTTG CAAGGTAAAG TTCAGAAGCA TCAGGCTTTT 
GAAGCAGAGC TTTCTGCTAA TCAGAGTCGT ATTGATGCAC TGGAGAAAGC TGGCCAGAAG 
TTGATTGATG TCAATCACTA TGCATCTGAT GAGGTGGCAG CTCGCATGAA TGAAGTCATC 
AGCTTGTGGA AGAAACTTCT GGAAGCCACC GAGCTCAAAG GTATAAAGCT GCGTGAAGCC 
AATCAACAGC AGCAATTTAA TCGCAATGTA GAAGACATTG AGTTGTGGCT GTATGAAGTG 
GAGGGTCACT TGGCTTCTGA TGATTATGGA AAAGATCTTA CCAGCGTTCA GAATCTTCAG 
AAGAAACATG CCCTGCTAGA GGCAGATGTT GCTGCCCATC AGGATCGGAT AGATGGCATT 
ACCATCCAGG CACGCCAGTT CCAAGATGCT GGGCACTTTG ATGCTGACAA TATCAAGAAG 
AAACAAGAAG CTTTAGTAGC TCGTTATGAA GCTCTGAAGG ATCCTATGGT GGCTCGCAAG 
CAGAAACTTG CAGATTCTCT TCGCCTGCAG CAGCTTTTCC GTGACATCGA GGATGAAGAG 
ACCTGGATCA GGGAAAAGGA ACCTATTGCA GCCTCAACAA ACCGAGGCAA GGACTTAATT 
GGTGTCCAGA ATCTGCTAAA GAAGCACCAG GCTTTGCAGG CAGAAATTGC AGGCCATGAG 
CCTCGCATTA AAGCAGTCAC ACAAAAAGGA AATGCGATGG TGGAAGAGGG ACATTTTGCT 
GCTGAAGATG TGAAGATCAA ATTGAATGAA CTAAACCAAA AATGGGACTC TCTGAAAGCC 
AAGGCATCTC AACGGCGACA GGATCTAGAG GATTCTCTGC AAGCTCAGCA GTATTTTGCT 
GATGCTAATG AGGCAGAATC ATGGATGAGG GAAAAGGAGC CCATTGTAGG CAGCACGGAT 
TATGGAAAGG ATGAAGACTC TGCTGAGGCT CTCCTGAAGA AGCATGAAGC TTTGATGTCT 
GATCTTTCTG CTTACGGCAG TAGCATACAG GCATTAAGGG AACAGGCCCA GTCCTGCAGG 
CAACAAGTTG CTCCCACTGA TGATGAAACT GGAAAAGAGC TTGTGCTAGC ACTCTATGAT 
TACCAAGAGA AGAGTCCTCG GGAGGTGACT ATGAAGAAAG GAGATATTCT AACCCTGCTC 
AACAGCACCA ACAAGGACTG GTGGAAGGTT GAAGTTAACG ATCGTCAGGG CTTTGTACCA 
GCTGCCTATG TGAAAAAACT AGATCCTGCC CAGTCTGCAT CCCGAGAGAA TCTTCTAGAA 
GAGCAAGGCA GCATAGCATT GCGACAGGAG CAAATTGACA ACCAGACTCT CATTACTAAG 
GAAGTCGGCA GTGTATCTCT GCGTATGAAA CAGGTCGAAG AACTGTATCA TTCCCTTCTT 
GAGCTGGGAG AAAAACGTAA AGGCATGCTA GAAAAAAGCT GCAAGAAGTT TATGCTTTTC 
CGTGAGGCTA ATGAGCTTCA ACAGTGGATC AATGAGAAGG AAGCTGCGCT CACTAATGAG 
GAAGTGGGTG CTGATTTGGA GCAGGTGGAG GTGCTACAGA AGAAGTTTGA TGATTTTCAG 
AAGGATTTAA AAGCTAATGA GTCACGACTG AAGGACATAA ACAAGGTTGC AAATGACCTG 
GAGTCTGAAG GGCTGATGGC AGAGGAAGTG CAAGCAGTAG AGCACCAGGA AGTCTATGGA 
ATGATGCCCA GAGATGAAAC TGATTCTAAG ACAGCCTCTC CTTGGAAGTC TGCACGTATG 
ATGGTACACA CAGTGGCAAC GTTTAACTCA ATCAAGGAAC TGAATGAACG CTGGAGATCC 
CTGCAGCAGT TGGCAGAGGA GCGAAGCCAG CTGTTGGGCA GCGCTCATGA AGTCCAGAGG 
TTCCACAGAG ATGCTGATGA AACAAAAGAA TGGATAGAAG AGAAGAATCA AGCATTAAAT 
ACCGACAACT ATGGACATGA CTTGGCCAGT GTGCAGGCTC TGCAGCGCAA GCATGAAGGC 
TTTGAGAGAG ACTTGGCAGC TCTTGGAGAC AAGGTGAATT CTCTTGGTGA AACTGCCCAG 
CGTCTGATCC AGTCACATCC AGAATCAGCT GAAGATCTTC AAGAAAAATG TACTGAGTTG 
AACCAAGCCT GGAGTAGTCT GGGAAAACGT GCTGACCAAC GCAAAGAGAA GCTTGGAGAT 
TCTCATGACC TGCAGCGTTT CCTCAGTGAT TTTAGAGACC TTATGTCTTG GATCAATGGA 
ATCCGGGGTC TGGTCTCCTC AGATGAACTT GCAAAAGATG TGACTGGAGC TGAAGCTCTG 
TTGGAAAGGC ATCAGGAGCA CCGTACTGAA ATAGATGCAC GGGCTGGCAC TTTTCAGGCA 
TTTGAACAGT TTGGACAGCA GCTTCTGGCT CGTGGACACT ATGCCAGCCC AGAGATTAAG 
GAGAAACTGG ATATTCTAGA TCAAGAACGG ACAGACCTAG AGAAGGCCTG GGTCCAGCGC 
AGAATGATGC TAGACCAGTG CTTAGAACTA CAGCTGTTTC ATCGGGATTG TGAACAAGCT 
GAAAACTGGA TGGCTGCCCG AGAGGCGTTC CTAAATACAG AAGACAAAGG AGACTCCTTA 
GACAGCGTGG AGGCACTCAT CAAGAAGCAT GAAGATTTCG ATAAAGCAAT CAATGTCCAG 
GAAGAGAAAA TTGCTGTCTT GCAGTCTTTT GCTGACCAAC TGATTGCTGC TGATCATTAT 
GCAAAAGGAG TCATTGCTAA CAGACGCAAT GAGGTTCTGG ACAGGTGGCG TCGTCTGAAG 
GCTCAGATGA TTGAGAAGAG ATCTAAGCTG GGAGAATCTC AGACCCTCCA ACAGTTCAGT 
CGTGATGTTG ATGAAATAGA AGCTTGGATC AGTGAAAAGC TTCAAACTGC AAGTGATGAG 
TCATATAAGG ATCCCACAAA CATCCAGCTT TCCAAACTGC TGAGCAAGCA CCAGAAGCAC 
CAAGCCTTTG AAGCTGAGCT CCACGCCAAC GCAGATCGGA TTCGTGGAGT CATTGAAATG 
GGGAACTCTC TTATTGAAAG AGGAGCGTGT GCTGGCAGCG AGGATGCTGT GAAGGCTCGG 
CTGGCTGCCC TGGCTGACCA ATGGGAGTTC CTGGTCCAGA AGTCATCAGA GAAGAGTCAG 
AAACTGAAAG AAGCAAATAA ACAGCAGAAT TTCAATACCG GCATCAAGGA CTTTGATTTC 
TGGCTTTCAG AGGTGGAAGC TTTGTTGGCA TCTGAAGACT ATGGGAAGGA CTTGGCATCA 
GTGAACAACC TTCTGAAGAA GCACCAATTA CTGGAAGCTG ATATATCTGC TCATGAGGAT 
CGGCTGAAGG ACCTGAACAG CCAGGCTGAC AGTTTGATGA CCAGCAGTGC TTTCGATACC 
TCCCAAGTAA AGGATAAACG TGAAACTATA AATGGGCGCT TCCAGAGAAT CAAGAGCATG 
GCAGCTGCCC GCCGTGCGAA GCTCAACGAG TCGCACCGCT TGCATCAGTT CTTCCGTGAC 
ATGGATGATG AGGAGTCCTG GATCAAAGAG AAGAAACTGT TGGTGAGCTC AGAGGACTAT 
GGCAGAGACC TGACTGGTGT GCAGAACCTG AGGAAGAAAC ATAAGCGCTT GGAAGCAGAA 
TTAGCTGCCC ATGAACCTGC TATCCAGGGT GTTCTAGACA CTGGCAAGAA GCTTTCAGAT 
GATAACACAA TTGGGAAGGA GGAGATACAG CAAAGACTGG CTCAGTTTGT GGACCACTGG 
AAAGAGTTAA AACAGCTGGC AGCTGCTCGG GGGCAGCGTC TGGAGGAGTC CCTGGAGTAC 
CAACAGTTTG TAGCAAATGT TGAGGAAGAG GAGGCATGGA TCAACGAGAA AATGACATTG 
GTAGCCAGTG AGGATTATGG AGACACACTT GCTGCTATCC AGGGCTTGCT GAAGAAGCAT 
GAAGCATTCG AGACTGACTT TACTGTCCAC AAAGACAGAG TGAATGATGT TTGCGCTAAT 
GGAGAGGATC TCATTAAAAA GAATAATCAC CATGTGGAGA ACATTACTGC TAAGATGAAG 
GGCCTCAAGG GCAAGGTATC AGATCTGGAG AAAGCCGCAG CTCAGAGGAA AGCCAAACTG 
GATGAGAACT CTGCCTTCCT CCAGTTCAAC TGGAAAGCAG ATGTGGTGGA GTCATGGATA 
GGTGAGAAGG AAAACAGTCT GAAGACAGAT GATTATGGCC GTGACCTCTC CTCTGTGCAA 
ACACTACTCA CCAAACAGGA AACCTTTGAT GCTGGACTTC AGGCTTTCCA GCAGGAGGGA 
ATTGCAAACA TCACTGCTCT GAAAGACCAG CTACTGGCAG CCAAACATAT CCAATCAAAG 
GCCATTGAGG TTCGTCACGC TTCCTTGATG AAACGCTGGA ATCAGCTGCT TGCTAATTCT 
GCAGCCAGGA AAAAGAAACT CTTGGAGGCT CAGGAGCACT TCAGAAAGGT TGAAGATCTC 
TTCTTGACTT TTGCCAAGAA GGCCTCTGCC TTCAACAGTT GGTTTGAGAA TGCTGAGGAG 
GACCTGACGG ATCCCGTGCG CTGCAATTCC CTGGAAGAAA TCAAAGCACT GCGAGAAGCC 
CACGATGCGT TCCGTTCCTC ACTTAGCTCT GCCCAAGCTG ACTTCAACCA GCTGGCAGAG 
CTTGATCGGC AGATCAAGAG CTTCCGTGTA GCCTCCAACC CGTACACTTG GTTTACTATG 
GAGGCTCTTG AAGAAACTTG GAGGAATCTG CAGAAAATTA TCAAGGAGCG TGAATTGGAG 
TTGCAGAAGG AGCAGCGGAG GCAGGAAGAA AATGACAAGT TGCGCCAGGA GTTTGCTCAG 
CATGCTAATG CCTTCCACCA GTGGATTCAG GAGACAAGGA CTTACCTGCT AGATGGCATA 
GCCTATCGTC GTGTCATTCG TGTCTATCAG TATGAAGTTG GGGATGATCT ATCTGGAAGG 
TCCTGCATGG TGGAGGAGTC GGGAACACTG GAATCCCAGC TGGAAGCTAC TAAACGCAAG 
CACCAGGAGA TCCGGGCTAT GAGGAGCCAG CTGAAGAAGA TTGAGGACCT TGGAGCAGCC 
ATGGAAGAGG CACTTATCTT GGACAACAAA TACACAGAGC ACAGCACCGT GGGGCTGGCG 
CAGCAGTGGG ACCAGCTTGA CCAGCTGGGA ATGAGAATGC AGCACAATCT GGAACAGCAG 
ATTCAAGCTC GAAACACAAC TGGAGTCACA GAGGAGGCCC TGAAGGAGTT CAGCATGATG 
TTCAAGCACT TTGACAAGGA CAAATCTGGA CGTCTTAATC ACCAGGAGTT TAAGTCTTGC 
TTGCGCTCCC TTGGCTATGA CCTGCCCATG GTTGAGGAAG GAGAGCCTGA CCCCGAATTT 
GAGTCTATTC TTGACACTGT TGATCCCAAC AGGGATGGCC ACGTCTCGCT GCAGGAGTAC 
ATGGCGTTCA TGATCAGCAG GGAAACAGAG AACGTGAAAT CCAGCGAGGA GATTGAGAGT 
GCTTTCCGTG CCCTCAGCTC GGAGGGGAAG CCTTACGTGA CCAAGGAGGA GCTCTACCAG 
AACCTGACCC GGGAGCAGGC CGACTACTGC ATTTCTCACA TGAAGCCCTA CATGGATGGC 
AAGGGCAGAG AACTTCCTTC TGCCTACGAC TACATAGAAT TTACACGTTC ACTCTTTGTG 
AATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015134770.1
CDS	110..7675
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSSLRRQKLEDSYRFQFFQRDADELEKWIQEKLQIASDENYKDPSNLQGKLQKHQAFEAEVQANSGAIVKLDETGNQMINEGHFASETIRSFTPYRTAQRPIVTATQVGSHIRTRLQELHRLWELLLEKMREKGVKLLQAQKLVQFLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDLAAHEERVNEVNQFAGKLIQEQHPEEELIKSKQDEVNASWQRLKGLAQQRQGKLFGAAEVQRFNRDVDETISWIKEKGQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPINASQIQVKREELIANWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFRSADESGQALLAAGHYASDEVKEKLTILSDERSALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMDDVATRRDALLSRRNALHERAMKRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYASDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTSVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDADNIKKKQEALVARYEALKDPMVARKQKLADSLRLQQLFRDIEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKIKLNELNQKWDSLKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPTDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTLITKEVGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTNEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVANDLESEGLMAEEVQAVEHQEVYGMMPRDETDSKTASPWKSARMMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAQRLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKEKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLARGHYASPEIKEKLDILDQERTDLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAVLQSFADQLIAADHYAKGVIANRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIEMGNSLIERGACAGSEDAVKARLAALADQWEFLVQKSSEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRETINGRFQRIKSMAAARRAKLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVDHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCANGEDLIKKNNHHVENITAKMKGLKGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHIQSKAIEVRHASLMKRWNQLLANSAARKKKLLEAQEHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGIAYRRVIRVYQYEVGDDLSGRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFESILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCISHMKPYMDGKGRELPSAYDYIEFTRSLFVN

Target ORF information:

RefSeq Version	XM_015279284.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279284.1

ORF Insert Sequence:

ATGGATCCAA GTGGGGTAAA AGTGTTGGAA ACAGCAGAAG ATATCCAAGA ACGGCGTCAG 
CAGGTTTTGG ACCGTTACCA CAGGTTCAAG GAGCTGTCTT CTCTGAGGCG CCAAAAACTC 
GAAGATTCCT ATCGGTTCCA GTTCTTCCAG CGCGATGCAG ATGAGCTGGA AAAATGGATC 
CAAGAGAAAC TCCAGATAGC ATCTGATGAA AATTACAAAG ACCCAAGCAA TTTACAGGGG 
AAGCTGCAGA AGCACCAGGC CTTTGAAGCT GAGGTGCAGG CCAATTCAGG GGCTATCGTT 
AAGCTGGATG AGACTGGAAA TCAGATGATC AATGAAGGCC ATTTTGCATC TGAAACCATA 
AGATCTTTCA CGCCTTACAG GACTGCACAG CGCCCAATAG TAACAGCCAC CCAAGTTGGG 
TCCCACATAC GAACTCGTCT ACAGGAGCTG CACCGACTAT GGGAGTTACT GCTGGAAAAA 
ATGAGAGAGA AGGGAGTGAA GCTGTTGCAA GCACAGAAGC TGGTGCAATT CTTACGGGAA 
TGTGAAGATG TCATGGACTG GATCAATGAC AAGGAAGCAA TAGTGACCTC AGAAGAGCTT 
GGACAGGACT TAGAGCATGT TGAGGTTTTG CAGAAGAAAT TTGAAGAGTT CCAAACAGAT 
CTGGCAGCTC ATGAGGAGAG AGTGAATGAA GTGAACCAGT TCGCTGGCAA ACTTATCCAG 
GAACAGCACC CTGAGGAGGA ACTTATAAAG TCCAAACAGG ATGAAGTAAA TGCAAGCTGG 
CAGCGTCTTA AGGGGCTTGC CCAACAGAGG CAAGGAAAAC TCTTTGGGGC AGCTGAAGTT 
CAGCGCTTCA ACAGGGATGT GGATGAAACT ATCAGCTGGA TTAAGGAGAA AGGGCAGTTG 
ATGGCCTCAG ATGACTTTGG CAGAGACTTG GCCAGCGTGC AAGCTTTACT ACGTAAGCAT 
GAAGGCCTGG AAAGAGATCT TGCAGCTTTG GAGGATAAGG TTAAGGCCCT CTGTGCAGAA 
GCTGACCGTT TGCAGCAGTC TCACCCTATA AATGCTTCTC AAATTCAAGT GAAACGGGAG 
GAACTGATTG CCAACTGGGA GCAGATCCGA ACTCTGGCAG CAGAGAGGCA TGCTCGCCTT 
AATGACTCCT ACAGGTTGCA GCGCTTTCTT GCAGATTTCC GGGACCTCAC TAGCTGGGTA 
ACTGAGATGA AGGCTCTGAT AAATGCTGAT GAGCTTGCCA ATGATGTGGC TGGAGCAGAA 
GCCCTTCTAG ATAGACATCA GGAACATAAG GGAGAAATTG ATGCTCATGA GGATAGCTTC 
AGATCTGCTG ATGAGTCTGG GCAGGCTTTG CTTGCAGCTG GGCACTATGC TTCTGATGAA 
GTTAAAGAAA AGCTGACTAT CCTCTCAGAT GAAAGATCAG CCTTGCTGGA ACTATGGGAG 
CTTCGCAGGC AACAGTATGA GCAGTGCATG GATCTGCAGC TTTTCTACAG AGATACTGAA 
CAAGTTGACA ACTGGATGAG CAAACAAGAA GCTTTTCTGC TGAACGAAGA CCTTGGTGAT 
TCTCTGGATA GCGTGGAGGC TCTTCTAAAG AAGCATGAAG ATTTTGAGAA ATCCCTAAGT 
GCTCAAGAGG AGAAGATCAC AGCACTGGAT GAGTTTGCTA CTAAACTGAT TCAAAATAAC 
CATTATGCCA TGGATGACGT TGCTACGCGC AGAGATGCTC TTCTGAGTCG CCGAAATGCT 
CTTCATGAAA GAGCCATGAA GCGCCGTGCC CAACTGGCAG ACTCTTTCCA TCTCCAGCAG 
TTTTTCAGGG ACTCTGATGA ACTGAAGAGT TGGGTTAATG AAAAAATGAA AACTGCTACC 
GATGAGGCTT ACAAGGATCC ATCCAACTTG CAAGGTAAAG TTCAGAAGCA TCAGGCTTTT 
GAAGCAGAGC TTTCTGCTAA TCAGAGTCGT ATTGATGCAC TGGAGAAAGC TGGCCAGAAG 
TTGATTGATG TCAATCACTA TGCATCTGAT GAGGTGGCAG CTCGCATGAA TGAAGTCATC 
AGCTTGTGGA AGAAACTTCT GGAAGCCACC GAGCTCAAAG GTATAAAGCT GCGTGAAGCC 
AATCAACAGC AGCAATTTAA TCGCAATGTA GAAGACATTG AGTTGTGGCT GTATGAAGTG 
GAGGGTCACT TGGCTTCTGA TGATTATGGA AAAGATCTTA CCAGCGTTCA GAATCTTCAG 
AAGAAACATG CCCTGCTAGA GGCAGATGTT GCTGCCCATC AGGATCGGAT AGATGGCATT 
ACCATCCAGG CACGCCAGTT CCAAGATGCT GGGCACTTTG ATGCTGACAA TATCAAGAAG 
AAACAAGAAG CTTTAGTAGC TCGTTATGAA GCTCTGAAGG ATCCTATGGT GGCTCGCAAG 
CAGAAACTTG CAGATTCTCT TCGCCTGCAG CAGCTTTTCC GTGACATCGA GGATGAAGAG 
ACCTGGATCA GGGAAAAGGA ACCTATTGCA GCCTCAACAA ACCGAGGCAA GGACTTAATT 
GGTGTCCAGA ATCTGCTAAA GAAGCACCAG GCTTTGCAGG CAGAAATTGC AGGCCATGAG 
CCTCGCATTA AAGCAGTCAC ACAAAAAGGA AATGCGATGG TGGAAGAGGG ACATTTTGCT 
GCTGAAGATG TGAAGATCAA ATTGAATGAA CTAAACCAAA AATGGGACTC TCTGAAAGCC 
AAGGCATCTC AACGGCGACA GGATCTAGAG GATTCTCTGC AAGCTCAGCA GTATTTTGCT 
GATGCTAATG AGGCAGAATC ATGGATGAGG GAAAAGGAGC CCATTGTAGG CAGCACGGAT 
TATGGAAAGG ATGAAGACTC TGCTGAGGCT CTCCTGAAGA AGCATGAAGC TTTGATGTCT 
GATCTTTCTG CTTACGGCAG TAGCATACAG GCATTAAGGG AACAGGCCCA GTCCTGCAGG 
CAACAAGTTG CTCCCACTGA TGATGAAACT GGAAAAGAGC TTGTGCTAGC ACTCTATGAT 
TACCAAGAGA AGAGTCCTCG GGAGGTGACT ATGAAGAAAG GAGATATTCT AACCCTGCTC 
AACAGCACCA ACAAGGACTG GTGGAAGGTT GAAGTTAACG ATCGTCAGGG CTTTGTACCA 
GCTGCCTATG TGAAAAAACT AGATCCTGCC CAGTCTGCAT CCCGAGAGAA TCTTCTAGAA 
GAGCAAGGCA GCATAGCATT GCGACAGGAG CAAATTGACA ACCAGACTCT CATTACTAAG 
GAAGTCGGCA GTGTATCTCT GCGTATGAAA CAGGTCGAAG AACTGTATCA TTCCCTTCTT 
GAGCTGGGAG AAAAACGTAA AGGCATGCTA GAAAAAAGCT GCAAGAAGTT TATGCTTTTC 
CGTGAGGCTA ATGAGCTTCA ACAGTGGATC AATGAGAAGG AAGCTGCGCT CACTAATGAG 
GAAGTGGGTG CTGATTTGGA GCAGGTGGAG GTGCTACAGA AGAAGTTTGA TGATTTTCAG 
AAGGATTTAA AAGCTAATGA GTCACGACTG AAGGACATAA ACAAGGTTGC AAATGACCTG 
GAGTCTGAAG GGCTGATGGC AGAGGAAGTG CAAGCAGTAG AGCACCAGGA AGTCTATGGA 
ATGATGCCCA GAGATGAAAC TGATTCTAAG ACAGCCTCTC CTTGGAAGTC TGCACGTATG 
ATGGTACACA CAGTGGCAAC GTTTAACTCA ATCAAGGAAC TGAATGAACG CTGGAGATCC 
CTGCAGCAGT TGGCAGAGGA GCGAAGCCAG CTGTTGGGCA GCGCTCATGA AGTCCAGAGG 
TTCCACAGAG ATGCTGATGA AACAAAAGAA TGGATAGAAG AGAAGAATCA AGCATTAAAT 
ACCGACAACT ATGGACATGA CTTGGCCAGT GTGCAGGCTC TGCAGCGCAA GCATGAAGGC 
TTTGAGAGAG ACTTGGCAGC TCTTGGAGAC AAGGTGAATT CTCTTGGTGA AACTGCCCAG 
CGTCTGATCC AGTCACATCC AGAATCAGCT GAAGATCTTC AAGAAAAATG TACTGAGTTG 
AACCAAGCCT GGAGTAGTCT GGGAAAACGT GCTGACCAAC GCAAAGAGAA GCTTGGAGAT 
TCTCATGACC TGCAGCGTTT CCTCAGTGAT TTTAGAGACC TTATGTCTTG GATCAATGGA 
ATCCGGGGTC TGGTCTCCTC AGATGAACTT GCAAAAGATG TGACTGGAGC TGAAGCTCTG 
TTGGAAAGGC ATCAGGAGCA CCGTACTGAA ATAGATGCAC GGGCTGGCAC TTTTCAGGCA 
TTTGAACAGT TTGGACAGCA GCTTCTGGCT CGTGGACACT ATGCCAGCCC AGAGATTAAG 
GAGAAACTGG ATATTCTAGA TCAAGAACGG ACAGACCTAG AGAAGGCCTG GGTCCAGCGC 
AGAATGATGC TAGACCAGTG CTTAGAACTA CAGCTGTTTC ATCGGGATTG TGAACAAGCT 
GAAAACTGGA TGGCTGCCCG AGAGGCGTTC CTAAATACAG AAGACAAAGG AGACTCCTTA 
GACAGCGTGG AGGCACTCAT CAAGAAGCAT GAAGATTTCG ATAAAGCAAT CAATGTCCAG 
GAAGAGAAAA TTGCTGTCTT GCAGTCTTTT GCTGACCAAC TGATTGCTGC TGATCATTAT 
GCAAAAGGAG TCATTGCTAA CAGACGCAAT GAGGTTCTGG ACAGGTGGCG TCGTCTGAAG 
GCTCAGATGA TTGAGAAGAG ATCTAAGCTG GGAGAATCTC AGACCCTCCA ACAGTTCAGT 
CGTGATGTTG ATGAAATAGA AGCTTGGATC AGTGAAAAGC TTCAAACTGC AAGTGATGAG 
TCATATAAGG ATCCCACAAA CATCCAGCTT TCCAAACTGC TGAGCAAGCA CCAGAAGCAC 
CAAGCCTTTG AAGCTGAGCT CCACGCCAAC GCAGATCGGA TTCGTGGAGT CATTGAAATG 
GGGAACTCTC TTATTGAAAG AGGAGCGTGT GCTGGCAGCG AGGATGCTGT GAAGGCTCGG 
CTGGCTGCCC TGGCTGACCA ATGGGAGTTC CTGGTCCAGA AGTCATCAGA GAAGAGTCAG 
AAACTGAAAG AAGCAAATAA ACAGCAGAAT TTCAATACCG GCATCAAGGA CTTTGATTTC 
TGGCTTTCAG AGGTGGAAGC TTTGTTGGCA TCTGAAGACT ATGGGAAGGA CTTGGCATCA 
GTGAACAACC TTCTGAAGAA GCACCAATTA CTGGAAGCTG ATATATCTGC TCATGAGGAT 
CGGCTGAAGG ACCTGAACAG CCAGGCTGAC AGTTTGATGA CCAGCAGTGC TTTCGATACC 
TCCCAAGTAA AGGATAAACG TGAAACTATA AATGGGCGCT TCCAGAGAAT CAAGAGCATG 
GCAGCTGCCC GCCGTGCGAA GCTCAACGAG TCGCACCGCT TGCATCAGTT CTTCCGTGAC 
ATGGATGATG AGGAGTCCTG GATCAAAGAG AAGAAACTGT TGGTGAGCTC AGAGGACTAT 
GGCAGAGACC TGACTGGTGT GCAGAACCTG AGGAAGAAAC ATAAGCGCTT GGAAGCAGAA 
TTAGCTGCCC ATGAACCTGC TATCCAGGGT GTTCTAGACA CTGGCAAGAA GCTTTCAGAT 
GATAACACAA TTGGGAAGGA GGAGATACAG CAAAGACTGG CTCAGTTTGT GGACCACTGG 
AAAGAGTTAA AACAGCTGGC AGCTGCTCGG GGGCAGCGTC TGGAGGAGTC CCTGGAGTAC 
CAACAGTTTG TAGCAAATGT TGAGGAAGAG GAGGCATGGA TCAACGAGAA AATGACATTG 
GTAGCCAGTG AGGATTATGG AGACACACTT GCTGCTATCC AGGGCTTGCT GAAGAAGCAT 
GAAGCATTCG AGACTGACTT TACTGTCCAC AAAGACAGAG TGAATGATGT TTGCGCTAAT 
GGAGAGGATC TCATTAAAAA GAATAATCAC CATGTGGAGA ACATTACTGC TAAGATGAAG 
GGCCTCAAGG GCAAGGTATC AGATCTGGAG AAAGCCGCAG CTCAGAGGAA AGCCAAACTG 
GATGAGAACT CTGCCTTCCT CCAGTTCAAC TGGAAAGCAG ATGTGGTGGA GTCATGGATA 
GGTGAGAAGG AAAACAGTCT GAAGACAGAT GATTATGGCC GTGACCTCTC CTCTGTGCAA 
ACACTACTCA CCAAACAGGA AACCTTTGAT GCTGGACTTC AGGCTTTCCA GCAGGAGGGA 
ATTGCAAACA TCACTGCTCT GAAAGACCAG CTACTGGCAG CCAAACATAT CCAATCAAAG 
GCCATTGAGG TTCGTCACGC TTCCTTGATG AAACGCTGGA ATCAGCTGCT TGCTAATTCT 
GCAGCCAGGA AAAAGAAACT CTTGGAGGCT CAGGAGCACT TCAGAAAGGT TGAAGATCTC 
TTCTTGACTT TTGCCAAGAA GGCCTCTGCC TTCAACAGTT GGTTTGAGAA TGCTGAGGAG 
GACCTGACGG ATCCCGTGCG CTGCAATTCC CTGGAAGAAA TCAAAGCACT GCGAGAAGCC 
CACGATGCGT TCCGTTCCTC ACTTAGCTCT GCCCAAGCTG ACTTCAACCA GCTGGCAGAG 
CTTGATCGGC AGATCAAGAG CTTCCGTGTA GCCTCCAACC CGTACACTTG GTTTACTATG 
GAGGCTCTTG AAGAAACTTG GAGGAATCTG CAGAAAATTA TCAAGGAGCG TGAATTGGAG 
TTGCAGAAGG AGCAGCGGAG GCAGGAAGAA AATGACAAGT TGCGCCAGGA GTTTGCTCAG 
CATGCTAATG CCTTCCACCA GTGGATTCAG GAGACAAGGA CTTACCTGCT AGATGGCATA 
GCCTATCGTC GTGTCATTCG TGTCTATCAG TATGAAGTTG GGGATGATCT ATCTGGAAGG 
TCCTGCATGG TGGAGGAGTC GGGAACACTG GAATCCCAGC TGGAAGCTAC TAAACGCAAG 
CACCAGGAGA TCCGGGCTAT GAGGAGCCAG CTGAAGAAGA TTGAGGACCT TGGAGCAGCC 
ATGGAAGAGG CACTTATCTT GGACAACAAA TACACAGAGC ACAGCACCGT GGGGCTGGCG 
CAGCAGTGGG ACCAGCTTGA CCAGCTGGGA ATGAGAATGC AGCACAATCT GGAACAGCAG 
ATTCAAGCTC GAAACACAAC TGGAGTCACA GAGGAGGCCC TGAAGGAGTT CAGCATGATG 
TTCAAGCACT TTGACAAGGA CAAATCTGGA CGTCTTAATC ACCAGGAGTT TAAGTCTTGC 
TTGCGCTCCC TTGGCTATGA CCTGCCCATG GTTGAGGAAG GAGAGCCTGA CCCCGAATTT 
GAGTCTATTC TTGACACTGT TGATCCCAAC AGGGATGGCC ACGTCTCGCT GCAGGAGTAC 
ATGGCGTTCA TGATCAGCAG GGAAACAGAG AACGTGAAAT CCAGCGAGGA GATTGAGAGT 
GCTTTCCGTG CCCTCAGCTC GGAGGGGAAG CCTTACGTGA CCAAGGAGGA GCTCTACCAG 
AACCTGACCC GGGAGCAGGC CGACTACTGC ATTTCTCACA TGAAGCCCTA CATGGATGGC 
AAGGGCAGAG AACTTCCTTC TGCCTACGAC TACATAGAAT TTACACGTTC ACTCTTTGTG 
AATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_015279284.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015134770.1 Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X3, mRNA.

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CloneID	OGa43310
Clone ID Related Accession (Same CDS sequence)	XM_015279284.1 , XM_015279284.2
Accession Version	XM_015279284.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7566bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015279284.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCAA GTGGGGTAAA AGTGTTGGAA ACAGCAGAAG ATATCCAAGA ACGGCGTCAG 
CAGGTTTTGG ACCGTTACCA CAGGTTCAAG GAGCTGTCTT CTCTGAGGCG CCAAAAACTC 
GAAGATTCCT ATCGGTTCCA GTTCTTCCAG CGCGATGCAG ATGAGCTGGA AAAATGGATC 
CAAGAGAAAC TCCAGATAGC ATCTGATGAA AATTACAAAG ACCCAAGCAA TTTACAGGGG 
AAGCTGCAGA AGCACCAGGC CTTTGAAGCT GAGGTGCAGG CCAATTCAGG GGCTATCGTT 
AAGCTGGATG AGACTGGAAA TCAGATGATC AATGAAGGCC ATTTTGCATC TGAAACCATA 
AGATCTTTCA CGCCTTACAG GACTGCACAG CGCCCAATAG TAACAGCCAC CCAAGTTGGG 
TCCCACATAC GAACTCGTCT ACAGGAGCTG CACCGACTAT GGGAGTTACT GCTGGAAAAA 
ATGAGAGAGA AGGGAGTGAA GCTGTTGCAA GCACAGAAGC TGGTGCAATT CTTACGGGAA 
TGTGAAGATG TCATGGACTG GATCAATGAC AAGGAAGCAA TAGTGACCTC AGAAGAGCTT 
GGACAGGACT TAGAGCATGT TGAGGTTTTG CAGAAGAAAT TTGAAGAGTT CCAAACAGAT 
CTGGCAGCTC ATGAGGAGAG AGTGAATGAA GTGAACCAGT TCGCTGGCAA ACTTATCCAG 
GAACAGCACC CTGAGGAGGA ACTTATAAAG TCCAAACAGG ATGAAGTAAA TGCAAGCTGG 
CAGCGTCTTA AGGGGCTTGC CCAACAGAGG CAAGGAAAAC TCTTTGGGGC AGCTGAAGTT 
CAGCGCTTCA ACAGGGATGT GGATGAAACT ATCAGCTGGA TTAAGGAGAA AGGGCAGTTG 
ATGGCCTCAG ATGACTTTGG CAGAGACTTG GCCAGCGTGC AAGCTTTACT ACGTAAGCAT 
GAAGGCCTGG AAAGAGATCT TGCAGCTTTG GAGGATAAGG TTAAGGCCCT CTGTGCAGAA 
GCTGACCGTT TGCAGCAGTC TCACCCTATA AATGCTTCTC AAATTCAAGT GAAACGGGAG 
GAACTGATTG CCAACTGGGA GCAGATCCGA ACTCTGGCAG CAGAGAGGCA TGCTCGCCTT 
AATGACTCCT ACAGGTTGCA GCGCTTTCTT GCAGATTTCC GGGACCTCAC TAGCTGGGTA 
ACTGAGATGA AGGCTCTGAT AAATGCTGAT GAGCTTGCCA ATGATGTGGC TGGAGCAGAA 
GCCCTTCTAG ATAGACATCA GGAACATAAG GGAGAAATTG ATGCTCATGA GGATAGCTTC 
AGATCTGCTG ATGAGTCTGG GCAGGCTTTG CTTGCAGCTG GGCACTATGC TTCTGATGAA 
GTTAAAGAAA AGCTGACTAT CCTCTCAGAT GAAAGATCAG CCTTGCTGGA ACTATGGGAG 
CTTCGCAGGC AACAGTATGA GCAGTGCATG GATCTGCAGC TTTTCTACAG AGATACTGAA 
CAAGTTGACA ACTGGATGAG CAAACAAGAA GCTTTTCTGC TGAACGAAGA CCTTGGTGAT 
TCTCTGGATA GCGTGGAGGC TCTTCTAAAG AAGCATGAAG ATTTTGAGAA ATCCCTAAGT 
GCTCAAGAGG AGAAGATCAC AGCACTGGAT GAGTTTGCTA CTAAACTGAT TCAAAATAAC 
CATTATGCCA TGGATGACGT TGCTACGCGC AGAGATGCTC TTCTGAGTCG CCGAAATGCT 
CTTCATGAAA GAGCCATGAA GCGCCGTGCC CAACTGGCAG ACTCTTTCCA TCTCCAGCAG 
TTTTTCAGGG ACTCTGATGA ACTGAAGAGT TGGGTTAATG AAAAAATGAA AACTGCTACC 
GATGAGGCTT ACAAGGATCC ATCCAACTTG CAAGGTAAAG TTCAGAAGCA TCAGGCTTTT 
GAAGCAGAGC TTTCTGCTAA TCAGAGTCGT ATTGATGCAC TGGAGAAAGC TGGCCAGAAG 
TTGATTGATG TCAATCACTA TGCATCTGAT GAGGTGGCAG CTCGCATGAA TGAAGTCATC 
AGCTTGTGGA AGAAACTTCT GGAAGCCACC GAGCTCAAAG GTATAAAGCT GCGTGAAGCC 
AATCAACAGC AGCAATTTAA TCGCAATGTA GAAGACATTG AGTTGTGGCT GTATGAAGTG 
GAGGGTCACT TGGCTTCTGA TGATTATGGA AAAGATCTTA CCAGCGTTCA GAATCTTCAG 
AAGAAACATG CCCTGCTAGA GGCAGATGTT GCTGCCCATC AGGATCGGAT AGATGGCATT 
ACCATCCAGG CACGCCAGTT CCAAGATGCT GGGCACTTTG ATGCTGACAA TATCAAGAAG 
AAACAAGAAG CTTTAGTAGC TCGTTATGAA GCTCTGAAGG ATCCTATGGT GGCTCGCAAG 
CAGAAACTTG CAGATTCTCT TCGCCTGCAG CAGCTTTTCC GTGACATCGA GGATGAAGAG 
ACCTGGATCA GGGAAAAGGA ACCTATTGCA GCCTCAACAA ACCGAGGCAA GGACTTAATT 
GGTGTCCAGA ATCTGCTAAA GAAGCACCAG GCTTTGCAGG CAGAAATTGC AGGCCATGAG 
CCTCGCATTA AAGCAGTCAC ACAAAAAGGA AATGCGATGG TGGAAGAGGG ACATTTTGCT 
GCTGAAGATG TGAAGATCAA ATTGAATGAA CTAAACCAAA AATGGGACTC TCTGAAAGCC 
AAGGCATCTC AACGGCGACA GGATCTAGAG GATTCTCTGC AAGCTCAGCA GTATTTTGCT 
GATGCTAATG AGGCAGAATC ATGGATGAGG GAAAAGGAGC CCATTGTAGG CAGCACGGAT 
TATGGAAAGG ATGAAGACTC TGCTGAGGCT CTCCTGAAGA AGCATGAAGC TTTGATGTCT 
GATCTTTCTG CTTACGGCAG TAGCATACAG GCATTAAGGG AACAGGCCCA GTCCTGCAGG 
CAACAAGTTG CTCCCACTGA TGATGAAACT GGAAAAGAGC TTGTGCTAGC ACTCTATGAT 
TACCAAGAGA AGAGTCCTCG GGAGGTGACT ATGAAGAAAG GAGATATTCT AACCCTGCTC 
AACAGCACCA ACAAGGACTG GTGGAAGGTT GAAGTTAACG ATCGTCAGGG CTTTGTACCA 
GCTGCCTATG TGAAAAAACT AGATCCTGCC CAGTCTGCAT CCCGAGAGAA TCTTCTAGAA 
GAGCAAGGCA GCATAGCATT GCGACAGGAG CAAATTGACA ACCAGACTCT CATTACTAAG 
GAAGTCGGCA GTGTATCTCT GCGTATGAAA CAGGTCGAAG AACTGTATCA TTCCCTTCTT 
GAGCTGGGAG AAAAACGTAA AGGCATGCTA GAAAAAAGCT GCAAGAAGTT TATGCTTTTC 
CGTGAGGCTA ATGAGCTTCA ACAGTGGATC AATGAGAAGG AAGCTGCGCT CACTAATGAG 
GAAGTGGGTG CTGATTTGGA GCAGGTGGAG GTGCTACAGA AGAAGTTTGA TGATTTTCAG 
AAGGATTTAA AAGCTAATGA GTCACGACTG AAGGACATAA ACAAGGTTGC AAATGACCTG 
GAGTCTGAAG GGCTGATGGC AGAGGAAGTG CAAGCAGTAG AGCACCAGGA AGTCTATGGA 
ATGATGCCCA GAGATGAAAC TGATTCTAAG ACAGCCTCTC CTTGGAAGTC TGCACGTATG 
ATGGTACACA CAGTGGCAAC GTTTAACTCA ATCAAGGAAC TGAATGAACG CTGGAGATCC 
CTGCAGCAGT TGGCAGAGGA GCGAAGCCAG CTGTTGGGCA GCGCTCATGA AGTCCAGAGG 
TTCCACAGAG ATGCTGATGA AACAAAAGAA TGGATAGAAG AGAAGAATCA AGCATTAAAT 
ACCGACAACT ATGGACATGA CTTGGCCAGT GTGCAGGCTC TGCAGCGCAA GCATGAAGGC 
TTTGAGAGAG ACTTGGCAGC TCTTGGAGAC AAGGTGAATT CTCTTGGTGA AACTGCCCAG 
CGTCTGATCC AGTCACATCC AGAATCAGCT GAAGATCTTC AAGAAAAATG TACTGAGTTG 
AACCAAGCCT GGAGTAGTCT GGGAAAACGT GCTGACCAAC GCAAAGAGAA GCTTGGAGAT 
TCTCATGACC TGCAGCGTTT CCTCAGTGAT TTTAGAGACC TTATGTCTTG GATCAATGGA 
ATCCGGGGTC TGGTCTCCTC AGATGAACTT GCAAAAGATG TGACTGGAGC TGAAGCTCTG 
TTGGAAAGGC ATCAGGAGCA CCGTACTGAA ATAGATGCAC GGGCTGGCAC TTTTCAGGCA 
TTTGAACAGT TTGGACAGCA GCTTCTGGCT CGTGGACACT ATGCCAGCCC AGAGATTAAG 
GAGAAACTGG ATATTCTAGA TCAAGAACGG ACAGACCTAG AGAAGGCCTG GGTCCAGCGC 
AGAATGATGC TAGACCAGTG CTTAGAACTA CAGCTGTTTC ATCGGGATTG TGAACAAGCT 
GAAAACTGGA TGGCTGCCCG AGAGGCGTTC CTAAATACAG AAGACAAAGG AGACTCCTTA 
GACAGCGTGG AGGCACTCAT CAAGAAGCAT GAAGATTTCG ATAAAGCAAT CAATGTCCAG 
GAAGAGAAAA TTGCTGTCTT GCAGTCTTTT GCTGACCAAC TGATTGCTGC TGATCATTAT 
GCAAAAGGAG TCATTGCTAA CAGACGCAAT GAGGTTCTGG ACAGGTGGCG TCGTCTGAAG 
GCTCAGATGA TTGAGAAGAG ATCTAAGCTG GGAGAATCTC AGACCCTCCA ACAGTTCAGT 
CGTGATGTTG ATGAAATAGA AGCTTGGATC AGTGAAAAGC TTCAAACTGC AAGTGATGAG 
TCATATAAGG ATCCCACAAA CATCCAGCTT TCCAAACTGC TGAGCAAGCA CCAGAAGCAC 
CAAGCCTTTG AAGCTGAGCT CCACGCCAAC GCAGATCGGA TTCGTGGAGT CATTGAAATG 
GGGAACTCTC TTATTGAAAG AGGAGCGTGT GCTGGCAGCG AGGATGCTGT GAAGGCTCGG 
CTGGCTGCCC TGGCTGACCA ATGGGAGTTC CTGGTCCAGA AGTCATCAGA GAAGAGTCAG 
AAACTGAAAG AAGCAAATAA ACAGCAGAAT TTCAATACCG GCATCAAGGA CTTTGATTTC 
TGGCTTTCAG AGGTGGAAGC TTTGTTGGCA TCTGAAGACT ATGGGAAGGA CTTGGCATCA 
GTGAACAACC TTCTGAAGAA GCACCAATTA CTGGAAGCTG ATATATCTGC TCATGAGGAT 
CGGCTGAAGG ACCTGAACAG CCAGGCTGAC AGTTTGATGA CCAGCAGTGC TTTCGATACC 
TCCCAAGTAA AGGATAAACG TGAAACTATA AATGGGCGCT TCCAGAGAAT CAAGAGCATG 
GCAGCTGCCC GCCGTGCGAA GCTCAACGAG TCGCACCGCT TGCATCAGTT CTTCCGTGAC 
ATGGATGATG AGGAGTCCTG GATCAAAGAG AAGAAACTGT TGGTGAGCTC AGAGGACTAT 
GGCAGAGACC TGACTGGTGT GCAGAACCTG AGGAAGAAAC ATAAGCGCTT GGAAGCAGAA 
TTAGCTGCCC ATGAACCTGC TATCCAGGGT GTTCTAGACA CTGGCAAGAA GCTTTCAGAT 
GATAACACAA TTGGGAAGGA GGAGATACAG CAAAGACTGG CTCAGTTTGT GGACCACTGG 
AAAGAGTTAA AACAGCTGGC AGCTGCTCGG GGGCAGCGTC TGGAGGAGTC CCTGGAGTAC 
CAACAGTTTG TAGCAAATGT TGAGGAAGAG GAGGCATGGA TCAACGAGAA AATGACATTG 
GTAGCCAGTG AGGATTATGG AGACACACTT GCTGCTATCC AGGGCTTGCT GAAGAAGCAT 
GAAGCATTCG AGACTGACTT TACTGTCCAC AAAGACAGAG TGAATGATGT TTGCGCTAAT 
GGAGAGGATC TCATTAAAAA GAATAATCAC CATGTGGAGA ACATTACTGC TAAGATGAAG 
GGCCTCAAGG GCAAGGTATC AGATCTGGAG AAAGCCGCAG CTCAGAGGAA AGCCAAACTG 
GATGAGAACT CTGCCTTCCT CCAGTTCAAC TGGAAAGCAG ATGTGGTGGA GTCATGGATA 
GGTGAGAAGG AAAACAGTCT GAAGACAGAT GATTATGGCC GTGACCTCTC CTCTGTGCAA 
ACACTACTCA CCAAACAGGA AACCTTTGAT GCTGGACTTC AGGCTTTCCA GCAGGAGGGA 
ATTGCAAACA TCACTGCTCT GAAAGACCAG CTACTGGCAG CCAAACATAT CCAATCAAAG 
GCCATTGAGG TTCGTCACGC TTCCTTGATG AAACGCTGGA ATCAGCTGCT TGCTAATTCT 
GCAGCCAGGA AAAAGAAACT CTTGGAGGCT CAGGAGCACT TCAGAAAGGT TGAAGATCTC 
TTCTTGACTT TTGCCAAGAA GGCCTCTGCC TTCAACAGTT GGTTTGAGAA TGCTGAGGAG 
GACCTGACGG ATCCCGTGCG CTGCAATTCC CTGGAAGAAA TCAAAGCACT GCGAGAAGCC 
CACGATGCGT TCCGTTCCTC ACTTAGCTCT GCCCAAGCTG ACTTCAACCA GCTGGCAGAG 
CTTGATCGGC AGATCAAGAG CTTCCGTGTA GCCTCCAACC CGTACACTTG GTTTACTATG 
GAGGCTCTTG AAGAAACTTG GAGGAATCTG CAGAAAATTA TCAAGGAGCG TGAATTGGAG 
TTGCAGAAGG AGCAGCGGAG GCAGGAAGAA AATGACAAGT TGCGCCAGGA GTTTGCTCAG 
CATGCTAATG CCTTCCACCA GTGGATTCAG GAGACAAGGA CTTACCTGCT AGATGGCATA 
GCCTATCGTC GTGTCATTCG TGTCTATCAG TATGAAGTTG GGGATGATCT ATCTGGAAGG 
TCCTGCATGG TGGAGGAGTC GGGAACACTG GAATCCCAGC TGGAAGCTAC TAAACGCAAG 
CACCAGGAGA TCCGGGCTAT GAGGAGCCAG CTGAAGAAGA TTGAGGACCT TGGAGCAGCC 
ATGGAAGAGG CACTTATCTT GGACAACAAA TACACAGAGC ACAGCACCGT GGGGCTGGCG 
CAGCAGTGGG ACCAGCTTGA CCAGCTGGGA ATGAGAATGC AGCACAATCT GGAACAGCAG 
ATTCAAGCTC GAAACACAAC TGGAGTCACA GAGGAGGCCC TGAAGGAGTT CAGCATGATG 
TTCAAGCACT TTGACAAGGA CAAATCTGGA CGTCTTAATC ACCAGGAGTT TAAGTCTTGC 
TTGCGCTCCC TTGGCTATGA CCTGCCCATG GTTGAGGAAG GAGAGCCTGA CCCCGAATTT 
GAGTCTATTC TTGACACTGT TGATCCCAAC AGGGATGGCC ACGTCTCGCT GCAGGAGTAC 
ATGGCGTTCA TGATCAGCAG GGAAACAGAG AACGTGAAAT CCAGCGAGGA GATTGAGAGT 
GCTTTCCGTG CCCTCAGCTC GGAGGGGAAG CCTTACGTGA CCAAGGAGGA GCTCTACCAG 
AACCTGACCC GGGAGCAGGC CGACTACTGC ATTTCTCACA TGAAGCCCTA CATGGATGGC 
AAGGGCAGAG AACTTCCTTC TGCCTACGAC TACATAGAAT TTACACGTTC ACTCTTTGTG 
AATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015134770.1
CDS	159..7724
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSSLRRQKLEDSYRFQFFQRDADELEKWIQEKLQIASDENYKDPSNLQGKLQKHQAFEAEVQANSGAIVKLDETGNQMINEGHFASETIRSFTPYRTAQRPIVTATQVGSHIRTRLQELHRLWELLLEKMREKGVKLLQAQKLVQFLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDLAAHEERVNEVNQFAGKLIQEQHPEEELIKSKQDEVNASWQRLKGLAQQRQGKLFGAAEVQRFNRDVDETISWIKEKGQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPINASQIQVKREELIANWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFRSADESGQALLAAGHYASDEVKEKLTILSDERSALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMDDVATRRDALLSRRNALHERAMKRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYASDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTSVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDADNIKKKQEALVARYEALKDPMVARKQKLADSLRLQQLFRDIEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKIKLNELNQKWDSLKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPTDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTLITKEVGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTNEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVANDLESEGLMAEEVQAVEHQEVYGMMPRDETDSKTASPWKSARMMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAQRLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKEKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLARGHYASPEIKEKLDILDQERTDLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAVLQSFADQLIAADHYAKGVIANRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIEMGNSLIERGACAGSEDAVKARLAALADQWEFLVQKSSEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRETINGRFQRIKSMAAARRAKLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVDHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCANGEDLIKKNNHHVENITAKMKGLKGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHIQSKAIEVRHASLMKRWNQLLANSAARKKKLLEAQEHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGIAYRRVIRVYQYEVGDDLSGRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFESILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCISHMKPYMDGKGRELPSAYDYIEFTRSLFVN

Target ORF information:

RefSeq Version	XM_015279284.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279284.2

ORF Insert Sequence:

ATGGATCCAA GTGGGGTAAA AGTGTTGGAA ACAGCAGAAG ATATCCAAGA ACGGCGTCAG 
CAGGTTTTGG ACCGTTACCA CAGGTTCAAG GAGCTGTCTT CTCTGAGGCG CCAAAAACTC 
GAAGATTCCT ATCGGTTCCA GTTCTTCCAG CGCGATGCAG ATGAGCTGGA AAAATGGATC 
CAAGAGAAAC TCCAGATAGC ATCTGATGAA AATTACAAAG ACCCAAGCAA TTTACAGGGG 
AAGCTGCAGA AGCACCAGGC CTTTGAAGCT GAGGTGCAGG CCAATTCAGG GGCTATCGTT 
AAGCTGGATG AGACTGGAAA TCAGATGATC AATGAAGGCC ATTTTGCATC TGAAACCATA 
AGATCTTTCA CGCCTTACAG GACTGCACAG CGCCCAATAG TAACAGCCAC CCAAGTTGGG 
TCCCACATAC GAACTCGTCT ACAGGAGCTG CACCGACTAT GGGAGTTACT GCTGGAAAAA 
ATGAGAGAGA AGGGAGTGAA GCTGTTGCAA GCACAGAAGC TGGTGCAATT CTTACGGGAA 
TGTGAAGATG TCATGGACTG GATCAATGAC AAGGAAGCAA TAGTGACCTC AGAAGAGCTT 
GGACAGGACT TAGAGCATGT TGAGGTTTTG CAGAAGAAAT TTGAAGAGTT CCAAACAGAT 
CTGGCAGCTC ATGAGGAGAG AGTGAATGAA GTGAACCAGT TCGCTGGCAA ACTTATCCAG 
GAACAGCACC CTGAGGAGGA ACTTATAAAG TCCAAACAGG ATGAAGTAAA TGCAAGCTGG 
CAGCGTCTTA AGGGGCTTGC CCAACAGAGG CAAGGAAAAC TCTTTGGGGC AGCTGAAGTT 
CAGCGCTTCA ACAGGGATGT GGATGAAACT ATCAGCTGGA TTAAGGAGAA AGGGCAGTTG 
ATGGCCTCAG ATGACTTTGG CAGAGACTTG GCCAGCGTGC AAGCTTTACT ACGTAAGCAT 
GAAGGCCTGG AAAGAGATCT TGCAGCTTTG GAGGATAAGG TTAAGGCCCT CTGTGCAGAA 
GCTGACCGTT TGCAGCAGTC TCACCCTATA AATGCTTCTC AAATTCAAGT GAAACGGGAG 
GAACTGATTG CCAACTGGGA GCAGATCCGA ACTCTGGCAG CAGAGAGGCA TGCTCGCCTT 
AATGACTCCT ACAGGTTGCA GCGCTTTCTT GCAGATTTCC GGGACCTCAC TAGCTGGGTA 
ACTGAGATGA AGGCTCTGAT AAATGCTGAT GAGCTTGCCA ATGATGTGGC TGGAGCAGAA 
GCCCTTCTAG ATAGACATCA GGAACATAAG GGAGAAATTG ATGCTCATGA GGATAGCTTC 
AGATCTGCTG ATGAGTCTGG GCAGGCTTTG CTTGCAGCTG GGCACTATGC TTCTGATGAA 
GTTAAAGAAA AGCTGACTAT CCTCTCAGAT GAAAGATCAG CCTTGCTGGA ACTATGGGAG 
CTTCGCAGGC AACAGTATGA GCAGTGCATG GATCTGCAGC TTTTCTACAG AGATACTGAA 
CAAGTTGACA ACTGGATGAG CAAACAAGAA GCTTTTCTGC TGAACGAAGA CCTTGGTGAT 
TCTCTGGATA GCGTGGAGGC TCTTCTAAAG AAGCATGAAG ATTTTGAGAA ATCCCTAAGT 
GCTCAAGAGG AGAAGATCAC AGCACTGGAT GAGTTTGCTA CTAAACTGAT TCAAAATAAC 
CATTATGCCA TGGATGACGT TGCTACGCGC AGAGATGCTC TTCTGAGTCG CCGAAATGCT 
CTTCATGAAA GAGCCATGAA GCGCCGTGCC CAACTGGCAG ACTCTTTCCA TCTCCAGCAG 
TTTTTCAGGG ACTCTGATGA ACTGAAGAGT TGGGTTAATG AAAAAATGAA AACTGCTACC 
GATGAGGCTT ACAAGGATCC ATCCAACTTG CAAGGTAAAG TTCAGAAGCA TCAGGCTTTT 
GAAGCAGAGC TTTCTGCTAA TCAGAGTCGT ATTGATGCAC TGGAGAAAGC TGGCCAGAAG 
TTGATTGATG TCAATCACTA TGCATCTGAT GAGGTGGCAG CTCGCATGAA TGAAGTCATC 
AGCTTGTGGA AGAAACTTCT GGAAGCCACC GAGCTCAAAG GTATAAAGCT GCGTGAAGCC 
AATCAACAGC AGCAATTTAA TCGCAATGTA GAAGACATTG AGTTGTGGCT GTATGAAGTG 
GAGGGTCACT TGGCTTCTGA TGATTATGGA AAAGATCTTA CCAGCGTTCA GAATCTTCAG 
AAGAAACATG CCCTGCTAGA GGCAGATGTT GCTGCCCATC AGGATCGGAT AGATGGCATT 
ACCATCCAGG CACGCCAGTT CCAAGATGCT GGGCACTTTG ATGCTGACAA TATCAAGAAG 
AAACAAGAAG CTTTAGTAGC TCGTTATGAA GCTCTGAAGG ATCCTATGGT GGCTCGCAAG 
CAGAAACTTG CAGATTCTCT TCGCCTGCAG CAGCTTTTCC GTGACATCGA GGATGAAGAG 
ACCTGGATCA GGGAAAAGGA ACCTATTGCA GCCTCAACAA ACCGAGGCAA GGACTTAATT 
GGTGTCCAGA ATCTGCTAAA GAAGCACCAG GCTTTGCAGG CAGAAATTGC AGGCCATGAG 
CCTCGCATTA AAGCAGTCAC ACAAAAAGGA AATGCGATGG TGGAAGAGGG ACATTTTGCT 
GCTGAAGATG TGAAGATCAA ATTGAATGAA CTAAACCAAA AATGGGACTC TCTGAAAGCC 
AAGGCATCTC AACGGCGACA GGATCTAGAG GATTCTCTGC AAGCTCAGCA GTATTTTGCT 
GATGCTAATG AGGCAGAATC ATGGATGAGG GAAAAGGAGC CCATTGTAGG CAGCACGGAT 
TATGGAAAGG ATGAAGACTC TGCTGAGGCT CTCCTGAAGA AGCATGAAGC TTTGATGTCT 
GATCTTTCTG CTTACGGCAG TAGCATACAG GCATTAAGGG AACAGGCCCA GTCCTGCAGG 
CAACAAGTTG CTCCCACTGA TGATGAAACT GGAAAAGAGC TTGTGCTAGC ACTCTATGAT 
TACCAAGAGA AGAGTCCTCG GGAGGTGACT ATGAAGAAAG GAGATATTCT AACCCTGCTC 
AACAGCACCA ACAAGGACTG GTGGAAGGTT GAAGTTAACG ATCGTCAGGG CTTTGTACCA 
GCTGCCTATG TGAAAAAACT AGATCCTGCC CAGTCTGCAT CCCGAGAGAA TCTTCTAGAA 
GAGCAAGGCA GCATAGCATT GCGACAGGAG CAAATTGACA ACCAGACTCT CATTACTAAG 
GAAGTCGGCA GTGTATCTCT GCGTATGAAA CAGGTCGAAG AACTGTATCA TTCCCTTCTT 
GAGCTGGGAG AAAAACGTAA AGGCATGCTA GAAAAAAGCT GCAAGAAGTT TATGCTTTTC 
CGTGAGGCTA ATGAGCTTCA ACAGTGGATC AATGAGAAGG AAGCTGCGCT CACTAATGAG 
GAAGTGGGTG CTGATTTGGA GCAGGTGGAG GTGCTACAGA AGAAGTTTGA TGATTTTCAG 
AAGGATTTAA AAGCTAATGA GTCACGACTG AAGGACATAA ACAAGGTTGC AAATGACCTG 
GAGTCTGAAG GGCTGATGGC AGAGGAAGTG CAAGCAGTAG AGCACCAGGA AGTCTATGGA 
ATGATGCCCA GAGATGAAAC TGATTCTAAG ACAGCCTCTC CTTGGAAGTC TGCACGTATG 
ATGGTACACA CAGTGGCAAC GTTTAACTCA ATCAAGGAAC TGAATGAACG CTGGAGATCC 
CTGCAGCAGT TGGCAGAGGA GCGAAGCCAG CTGTTGGGCA GCGCTCATGA AGTCCAGAGG 
TTCCACAGAG ATGCTGATGA AACAAAAGAA TGGATAGAAG AGAAGAATCA AGCATTAAAT 
ACCGACAACT ATGGACATGA CTTGGCCAGT GTGCAGGCTC TGCAGCGCAA GCATGAAGGC 
TTTGAGAGAG ACTTGGCAGC TCTTGGAGAC AAGGTGAATT CTCTTGGTGA AACTGCCCAG 
CGTCTGATCC AGTCACATCC AGAATCAGCT GAAGATCTTC AAGAAAAATG TACTGAGTTG 
AACCAAGCCT GGAGTAGTCT GGGAAAACGT GCTGACCAAC GCAAAGAGAA GCTTGGAGAT 
TCTCATGACC TGCAGCGTTT CCTCAGTGAT TTTAGAGACC TTATGTCTTG GATCAATGGA 
ATCCGGGGTC TGGTCTCCTC AGATGAACTT GCAAAAGATG TGACTGGAGC TGAAGCTCTG 
TTGGAAAGGC ATCAGGAGCA CCGTACTGAA ATAGATGCAC GGGCTGGCAC TTTTCAGGCA 
TTTGAACAGT TTGGACAGCA GCTTCTGGCT CGTGGACACT ATGCCAGCCC AGAGATTAAG 
GAGAAACTGG ATATTCTAGA TCAAGAACGG ACAGACCTAG AGAAGGCCTG GGTCCAGCGC 
AGAATGATGC TAGACCAGTG CTTAGAACTA CAGCTGTTTC ATCGGGATTG TGAACAAGCT 
GAAAACTGGA TGGCTGCCCG AGAGGCGTTC CTAAATACAG AAGACAAAGG AGACTCCTTA 
GACAGCGTGG AGGCACTCAT CAAGAAGCAT GAAGATTTCG ATAAAGCAAT CAATGTCCAG 
GAAGAGAAAA TTGCTGTCTT GCAGTCTTTT GCTGACCAAC TGATTGCTGC TGATCATTAT 
GCAAAAGGAG TCATTGCTAA CAGACGCAAT GAGGTTCTGG ACAGGTGGCG TCGTCTGAAG 
GCTCAGATGA TTGAGAAGAG ATCTAAGCTG GGAGAATCTC AGACCCTCCA ACAGTTCAGT 
CGTGATGTTG ATGAAATAGA AGCTTGGATC AGTGAAAAGC TTCAAACTGC AAGTGATGAG 
TCATATAAGG ATCCCACAAA CATCCAGCTT TCCAAACTGC TGAGCAAGCA CCAGAAGCAC 
CAAGCCTTTG AAGCTGAGCT CCACGCCAAC GCAGATCGGA TTCGTGGAGT CATTGAAATG 
GGGAACTCTC TTATTGAAAG AGGAGCGTGT GCTGGCAGCG AGGATGCTGT GAAGGCTCGG 
CTGGCTGCCC TGGCTGACCA ATGGGAGTTC CTGGTCCAGA AGTCATCAGA GAAGAGTCAG 
AAACTGAAAG AAGCAAATAA ACAGCAGAAT TTCAATACCG GCATCAAGGA CTTTGATTTC 
TGGCTTTCAG AGGTGGAAGC TTTGTTGGCA TCTGAAGACT ATGGGAAGGA CTTGGCATCA 
GTGAACAACC TTCTGAAGAA GCACCAATTA CTGGAAGCTG ATATATCTGC TCATGAGGAT 
CGGCTGAAGG ACCTGAACAG CCAGGCTGAC AGTTTGATGA CCAGCAGTGC TTTCGATACC 
TCCCAAGTAA AGGATAAACG TGAAACTATA AATGGGCGCT TCCAGAGAAT CAAGAGCATG 
GCAGCTGCCC GCCGTGCGAA GCTCAACGAG TCGCACCGCT TGCATCAGTT CTTCCGTGAC 
ATGGATGATG AGGAGTCCTG GATCAAAGAG AAGAAACTGT TGGTGAGCTC AGAGGACTAT 
GGCAGAGACC TGACTGGTGT GCAGAACCTG AGGAAGAAAC ATAAGCGCTT GGAAGCAGAA 
TTAGCTGCCC ATGAACCTGC TATCCAGGGT GTTCTAGACA CTGGCAAGAA GCTTTCAGAT 
GATAACACAA TTGGGAAGGA GGAGATACAG CAAAGACTGG CTCAGTTTGT GGACCACTGG 
AAAGAGTTAA AACAGCTGGC AGCTGCTCGG GGGCAGCGTC TGGAGGAGTC CCTGGAGTAC 
CAACAGTTTG TAGCAAATGT TGAGGAAGAG GAGGCATGGA TCAACGAGAA AATGACATTG 
GTAGCCAGTG AGGATTATGG AGACACACTT GCTGCTATCC AGGGCTTGCT GAAGAAGCAT 
GAAGCATTCG AGACTGACTT TACTGTCCAC AAAGACAGAG TGAATGATGT TTGCGCTAAT 
GGAGAGGATC TCATTAAAAA GAATAATCAC CATGTGGAGA ACATTACTGC TAAGATGAAG 
GGCCTCAAGG GCAAGGTATC AGATCTGGAG AAAGCCGCAG CTCAGAGGAA AGCCAAACTG 
GATGAGAACT CTGCCTTCCT CCAGTTCAAC TGGAAAGCAG ATGTGGTGGA GTCATGGATA 
GGTGAGAAGG AAAACAGTCT GAAGACAGAT GATTATGGCC GTGACCTCTC CTCTGTGCAA 
ACACTACTCA CCAAACAGGA AACCTTTGAT GCTGGACTTC AGGCTTTCCA GCAGGAGGGA 
ATTGCAAACA TCACTGCTCT GAAAGACCAG CTACTGGCAG CCAAACATAT CCAATCAAAG 
GCCATTGAGG TTCGTCACGC TTCCTTGATG AAACGCTGGA ATCAGCTGCT TGCTAATTCT 
GCAGCCAGGA AAAAGAAACT CTTGGAGGCT CAGGAGCACT TCAGAAAGGT TGAAGATCTC 
TTCTTGACTT TTGCCAAGAA GGCCTCTGCC TTCAACAGTT GGTTTGAGAA TGCTGAGGAG 
GACCTGACGG ATCCCGTGCG CTGCAATTCC CTGGAAGAAA TCAAAGCACT GCGAGAAGCC 
CACGATGCGT TCCGTTCCTC ACTTAGCTCT GCCCAAGCTG ACTTCAACCA GCTGGCAGAG 
CTTGATCGGC AGATCAAGAG CTTCCGTGTA GCCTCCAACC CGTACACTTG GTTTACTATG 
GAGGCTCTTG AAGAAACTTG GAGGAATCTG CAGAAAATTA TCAAGGAGCG TGAATTGGAG 
TTGCAGAAGG AGCAGCGGAG GCAGGAAGAA AATGACAAGT TGCGCCAGGA GTTTGCTCAG 
CATGCTAATG CCTTCCACCA GTGGATTCAG GAGACAAGGA CTTACCTGCT AGATGGCATA 
GCCTATCGTC GTGTCATTCG TGTCTATCAG TATGAAGTTG GGGATGATCT ATCTGGAAGG 
TCCTGCATGG TGGAGGAGTC GGGAACACTG GAATCCCAGC TGGAAGCTAC TAAACGCAAG 
CACCAGGAGA TCCGGGCTAT GAGGAGCCAG CTGAAGAAGA TTGAGGACCT TGGAGCAGCC 
ATGGAAGAGG CACTTATCTT GGACAACAAA TACACAGAGC ACAGCACCGT GGGGCTGGCG 
CAGCAGTGGG ACCAGCTTGA CCAGCTGGGA ATGAGAATGC AGCACAATCT GGAACAGCAG 
ATTCAAGCTC GAAACACAAC TGGAGTCACA GAGGAGGCCC TGAAGGAGTT CAGCATGATG 
TTCAAGCACT TTGACAAGGA CAAATCTGGA CGTCTTAATC ACCAGGAGTT TAAGTCTTGC 
TTGCGCTCCC TTGGCTATGA CCTGCCCATG GTTGAGGAAG GAGAGCCTGA CCCCGAATTT 
GAGTCTATTC TTGACACTGT TGATCCCAAC AGGGATGGCC ACGTCTCGCT GCAGGAGTAC 
ATGGCGTTCA TGATCAGCAG GGAAACAGAG AACGTGAAAT CCAGCGAGGA GATTGAGAGT 
GCTTTCCGTG CCCTCAGCTC GGAGGGGAAG CCTTACGTGA CCAAGGAGGA GCTCTACCAG 
AACCTGACCC GGGAGCAGGC CGACTACTGC ATTTCTCACA TGAAGCCCTA CATGGATGGC 
AAGGGCAGAG AACTTCCTTC TGCCTACGAC TACATAGAAT TTACACGTTC ACTCTTTGTG 
AATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_015279285.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015134771.1 Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X4, mRNA.

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CloneID	OGa43311
Clone ID Related Accession (Same CDS sequence)	XM_015279285.2 , XM_015279285.1
Accession Version	XM_015279285.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7542bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015279285.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG 
TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC 
TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT 
GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT 
GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG 
ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAT CTTTCACGCC TTACAGGACT 
GCACAGCGCC CAATAGTAAC AGCCACCCAA GTTGGGTCCC ACATACGAAC TCGTCTACAG 
GAGCTGCACC GACTATGGGA GTTACTGCTG GAAAAAATGA GAGAGAAGGG AGTGAAGCTG 
TTGCAAGCAC AGAAGCTGGT GCAATTCTTA CGGGAATGTG AAGATGTCAT GGACTGGATC 
AATGACAAGG AAGCAATAGT GACCTCAGAA GAGCTTGGAC AGGACTTAGA GCATGTTGAG 
GTTTTGCAGA AGAAATTTGA AGAGTTCCAA ACAGATCTGG CAGCTCATGA GGAGAGAGTG 
AATGAAGTGA ACCAGTTCGC TGGCAAACTT ATCCAGGAAC AGCACCCTGA GGAGGAACTT 
ATAAAGTCCA AACAGGATGA AGTAAATGCA AGCTGGCAGC GTCTTAAGGG GCTTGCCCAA 
CAGAGGCAAG GAAAACTCTT TGGGGCAGCT GAAGTTCAGC GCTTCAACAG GGATGTGGAT 
GAAACTATCA GCTGGATTAA GGAGAAAGGG CAGTTGATGG CCTCAGATGA CTTTGGCAGA 
GACTTGGCCA GCGTGCAAGC TTTACTACGT AAGCATGAAG GCCTGGAAAG AGATCTTGCA 
GCTTTGGAGG ATAAGGTTAA GGCCCTCTGT GCAGAAGCTG ACCGTTTGCA GCAGTCTCAC 
CCTATAAATG CTTCTCAAAT TCAAGTGAAA CGGGAGGAAC TGATTGCCAA CTGGGAGCAG 
ATCCGAACTC TGGCAGCAGA GAGGCATGCT CGCCTTAATG ACTCCTACAG GTTGCAGCGC 
TTTCTTGCAG ATTTCCGGGA CCTCACTAGC TGGGTAACTG AGATGAAGGC TCTGATAAAT 
GCTGATGAGC TTGCCAATGA TGTGGCTGGA GCAGAAGCCC TTCTAGATAG ACATCAGGAA 
CATAAGGGAG AAATTGATGC TCATGAGGAT AGCTTCAGAT CTGCTGATGA GTCTGGGCAG 
GCTTTGCTTG CAGCTGGGCA CTATGCTTCT GATGAAGTTA AAGAAAAGCT GACTATCCTC 
TCAGATGAAA GATCAGCCTT GCTGGAACTA TGGGAGCTTC GCAGGCAACA GTATGAGCAG 
TGCATGGATC TGCAGCTTTT CTACAGAGAT ACTGAACAAG TTGACAACTG GATGAGCAAA 
CAAGAAGCTT TTCTGCTGAA CGAAGACCTT GGTGATTCTC TGGATAGCGT GGAGGCTCTT 
CTAAAGAAGC ATGAAGATTT TGAGAAATCC CTAAGTGCTC AAGAGGAGAA GATCACAGCA 
CTGGATGAGT TTGCTACTAA ACTGATTCAA AATAACCATT ATGCCATGGA TGACGTTGCT 
ACGCGCAGAG ATGCTCTTCT GAGTCGCCGA AATGCTCTTC ATGAAAGAGC CATGAAGCGC 
CGTGCCCAAC TGGCAGACTC TTTCCATCTC CAGCAGTTTT TCAGGGACTC TGATGAACTG 
AAGAGTTGGG TTAATGAAAA AATGAAAACT GCTACCGATG AGGCTTACAA GGATCCATCC 
AACTTGCAAG GTAAAGTTCA GAAGCATCAG GCTTTTGAAG CAGAGCTTTC TGCTAATCAG 
AGTCGTATTG ATGCACTGGA GAAAGCTGGC CAGAAGTTGA TTGATGTCAA TCACTATGCA 
TCTGATGAGG TGGCAGCTCG CATGAATGAA GTCATCAGCT TGTGGAAGAA ACTTCTGGAA 
GCCACCGAGC TCAAAGGTAT AAAGCTGCGT GAAGCCAATC AACAGCAGCA ATTTAATCGC 
AATGTAGAAG ACATTGAGTT GTGGCTGTAT GAAGTGGAGG GTCACTTGGC TTCTGATGAT 
TATGGAAAAG ATCTTACCAG CGTTCAGAAT CTTCAGAAGA AACATGCCCT GCTAGAGGCA 
GATGTTGCTG CCCATCAGGA TCGGATAGAT GGCATTACCA TCCAGGCACG CCAGTTCCAA 
GATGCTGGGC ACTTTGATGC TGACAATATC AAGAAGAAAC AAGAAGCTTT AGTAGCTCGT 
TATGAAGCTC TGAAGGATCC TATGGTGGCT CGCAAGCAGA AACTTGCAGA TTCTCTTCGC 
CTGCAGCAGC TTTTCCGTGA CATCGAGGAT GAAGAGACCT GGATCAGGGA AAAGGAACCT 
ATTGCAGCCT CAACAAACCG AGGCAAGGAC TTAATTGGTG TCCAGAATCT GCTAAAGAAG 
CACCAGGCTT TGCAGGCAGA AATTGCAGGC CATGAGCCTC GCATTAAAGC AGTCACACAA 
AAAGGAAATG CGATGGTGGA AGAGGGACAT TTTGCTGCTG AAGATGTGAA GATCAAATTG 
AATGAACTAA ACCAAAAATG GGACTCTCTG AAAGCCAAGG CATCTCAACG GCGACAGGAT 
CTAGAGGATT CTCTGCAAGC TCAGCAGTAT TTTGCTGATG CTAATGAGGC AGAATCATGG 
ATGAGGGAAA AGGAGCCCAT TGTAGGCAGC ACGGATTATG GAAAGGATGA AGACTCTGCT 
GAGGCTCTCC TGAAGAAGCA TGAAGCTTTG ATGTCTGATC TTTCTGCTTA CGGCAGTAGC 
ATACAGGCAT TAAGGGAACA GGCCCAGTCC TGCAGGCAAC AAGTTGCTCC CACTGATGAT 
GAAACTGGAA AAGAGCTTGT GCTAGCACTC TATGATTACC AAGAGAAGAG TCCTCGGGAG 
GTGACTATGA AGAAAGGAGA TATTCTAACC CTGCTCAACA GCACCAACAA GGACTGGTGG 
AAGGTTGAAG TTAACGATCG TCAGGGCTTT GTACCAGCTG CCTATGTGAA AAAACTAGAT 
CCTGCCCAGT CTGCATCCCG AGAGAATCTT CTAGAAGAGC AAGGCAGCAT AGCATTGCGA 
CAGGAGCAAA TTGACAACCA GTATCATTCC CTTCTTGAGC TGGGAGAAAA ACGTAAAGGC 
ATGCTAGAAA AAAGCTGCAA GAAGTTTATG CTTTTCCGTG AGGCTAATGA GCTTCAACAG 
TGGATCAATG AGAAGGAAGC TGCGCTCACT AATGAGGAAG TGGGTGCTGA TTTGGAGCAG 
GTGGAGGTGC TACAGAAGAA GTTTGATGAT TTTCAGAAGG ATTTAAAAGC TAATGAGTCA 
CGACTGAAGG ACATAAACAA GGTTGCAAAT GACCTGGAGT CTGAAGGGCT GATGGCAGAG 
GAAGTGCAAG CAGTAGAGCA CCAGGAAGTC TATGGAATGA TGCCCAGAGA TGAAACTGAT 
TCTAAGACAG CCTCTCCTTG GAAGTCTGCA CGTATGATGG TACACACAGT GGCAACGTTT 
AACTCAATCA AGGAACTGAA TGAACGCTGG AGATCCCTGC AGCAGTTGGC AGAGGAGCGA 
AGCCAGCTGT TGGGCAGCGC TCATGAAGTC CAGAGGTTCC ACAGAGATGC TGATGAAACA 
AAAGAATGGA TAGAAGAGAA GAATCAAGCA TTAAATACCG ACAACTATGG ACATGACTTG 
GCCAGTGTGC AGGCTCTGCA GCGCAAGCAT GAAGGCTTTG AGAGAGACTT GGCAGCTCTT 
GGAGACAAGG TGAATTCTCT TGGTGAAACT GCCCAGCGTC TGATCCAGTC ACATCCAGAA 
TCAGCTGAAG ATCTTCAAGA AAAATGTACT GAGTTGAACC AAGCCTGGAG TAGTCTGGGA 
AAACGTGCTG ACCAACGCAA AGAGAAGCTT GGAGATTCTC ATGACCTGCA GCGTTTCCTC 
AGTGATTTTA GAGACCTTAT GTCTTGGATC AATGGAATCC GGGGTCTGGT CTCCTCAGAT 
GAACTTGCAA AAGATGTGAC TGGAGCTGAA GCTCTGTTGG AAAGGCATCA GGAGCACCGT 
ACTGAAATAG ATGCACGGGC TGGCACTTTT CAGGCATTTG AACAGTTTGG ACAGCAGCTT 
CTGGCTCGTG GACACTATGC CAGCCCAGAG ATTAAGGAGA AACTGGATAT TCTAGATCAA 
GAACGGACAG ACCTAGAGAA GGCCTGGGTC CAGCGCAGAA TGATGCTAGA CCAGTGCTTA 
GAACTACAGC TGTTTCATCG GGATTGTGAA CAAGCTGAAA ACTGGATGGC TGCCCGAGAG 
GCGTTCCTAA ATACAGAAGA CAAAGGAGAC TCCTTAGACA GCGTGGAGGC ACTCATCAAG 
AAGCATGAAG ATTTCGATAA AGCAATCAAT GTCCAGGAAG AGAAAATTGC TGTCTTGCAG 
TCTTTTGCTG ACCAACTGAT TGCTGCTGAT CATTATGCAA AAGGAGTCAT TGCTAACAGA 
CGCAATGAGG TTCTGGACAG GTGGCGTCGT CTGAAGGCTC AGATGATTGA GAAGAGATCT 
AAGCTGGGAG AATCTCAGAC CCTCCAACAG TTCAGTCGTG ATGTTGATGA AATAGAAGCT 
TGGATCAGTG AAAAGCTTCA AACTGCAAGT GATGAGTCAT ATAAGGATCC CACAAACATC 
CAGCTTTCCA AACTGCTGAG CAAGCACCAG AAGCACCAAG CCTTTGAAGC TGAGCTCCAC 
GCCAACGCAG ATCGGATTCG TGGAGTCATT GAAATGGGGA ACTCTCTTAT TGAAAGAGGA 
GCGTGTGCTG GCAGCGAGGA TGCTGTGAAG GCTCGGCTGG CTGCCCTGGC TGACCAATGG 
GAGTTCCTGG TCCAGAAGTC ATCAGAGAAG AGTCAGAAAC TGAAAGAAGC AAATAAACAG 
CAGAATTTCA ATACCGGCAT CAAGGACTTT GATTTCTGGC TTTCAGAGGT GGAAGCTTTG 
TTGGCATCTG AAGACTATGG GAAGGACTTG GCATCAGTGA ACAACCTTCT GAAGAAGCAC 
CAATTACTGG AAGCTGATAT ATCTGCTCAT GAGGATCGGC TGAAGGACCT GAACAGCCAG 
GCTGACAGTT TGATGACCAG CAGTGCTTTC GATACCTCCC AAGTAAAGGA TAAACGTGAA 
ACTATAAATG GGCGCTTCCA GAGAATCAAG AGCATGGCAG CTGCCCGCCG TGCGAAGCTC 
AACGAGTCGC ACCGCTTGCA TCAGTTCTTC CGTGACATGG ATGATGAGGA GTCCTGGATC 
AAAGAGAAGA AACTGTTGGT GAGCTCAGAG GACTATGGCA GAGACCTGAC TGGTGTGCAG 
AACCTGAGGA AGAAACATAA GCGCTTGGAA GCAGAATTAG CTGCCCATGA ACCTGCTATC 
CAGGGTGTTC TAGACACTGG CAAGAAGCTT TCAGATGATA ACACAATTGG GAAGGAGGAG 
ATACAGCAAA GACTGGCTCA GTTTGTGGAC CACTGGAAAG AGTTAAAACA GCTGGCAGCT 
GCTCGGGGGC AGCGTCTGGA GGAGTCCCTG GAGTACCAAC AGTTTGTAGC AAATGTTGAG 
GAAGAGGAGG CATGGATCAA CGAGAAAATG ACATTGGTAG CCAGTGAGGA TTATGGAGAC 
ACACTTGCTG CTATCCAGGG CTTGCTGAAG AAGCATGAAG CATTCGAGAC TGACTTTACT 
GTCCACAAAG ACAGAGTGAA TGATGTTTGC GCTAATGGAG AGGATCTCAT TAAAAAGAAT 
AATCACCATG TGGAGAACAT TACTGCTAAG ATGAAGGGCC TCAAGGGCAA GGTATCAGAT 
CTGGAGAAAG CCGCAGCTCA GAGGAAAGCC AAACTGGATG AGAACTCTGC CTTCCTCCAG 
TTCAACTGGA AAGCAGATGT GGTGGAGTCA TGGATAGGTG AGAAGGAAAA CAGTCTGAAG 
ACAGATGATT ATGGCCGTGA CCTCTCCTCT GTGCAAACAC TACTCACCAA ACAGGAAACC 
TTTGATGCTG GACTTCAGGC TTTCCAGCAG GAGGGAATTG CAAACATCAC TGCTCTGAAA 
GACCAGCTAC TGGCAGCCAA ACATATCCAA TCAAAGGCCA TTGAGGTTCG TCACGCTTCC 
TTGATGAAAC GCTGGAATCA GCTGCTTGCT AATTCTGCAG CCAGGAAAAA GAAACTCTTG 
GAGGCTCAGG AGCACTTCAG AAAGGTTGAA GATCTCTTCT TGACTTTTGC CAAGAAGGCC 
TCTGCCTTCA ACAGTTGGTT TGAGAATGCT GAGGAGGACC TGACGGATCC CGTGCGCTGC 
AATTCCCTGG AAGAAATCAA AGCACTGCGA GAAGCCCACG ATGCGTTCCG TTCCTCACTT 
AGCTCTGCCC AAGCTGACTT CAACCAGCTG GCAGAGCTTG ATCGGCAGAT CAAGAGCTTC 
CGTGTAGCCT CCAACCCGTA CACTTGGTTT ACTATGGAGG CTCTTGAAGA AACTTGGAGG 
AATCTGCAGA AAATTATCAA GGAGCGTGAA TTGGAGTTGC AGAAGGAGCA GCGGAGGCAG 
GAAGAAAATG ACAAGTTGCG CCAGGAGTTT GCTCAGCATG CTAATGCCTT CCACCAGTGG 
ATTCAGGAGA CAAGGACTTA CCTGCTAGAT GGCATAGCCT ATCGTCGTGT CATTCGTGTC 
TATCAGTATG AAGTTGGGGA TGATCTATCT GGAAGGTCCT GCATGGTGGA GGAGTCGGGA 
ACACTGGAAT CCCAGCTGGA AGCTACTAAA CGCAAGCACC AGGAGATCCG GGCTATGAGG 
AGCCAGCTGA AGAAGATTGA GGACCTTGGA GCAGCCATGG AAGAGGCACT TATCTTGGAC 
AACAAATACA CAGAGCACAG CACCGTGGGG CTGGCGCAGC AGTGGGACCA GCTTGACCAG 
CTGGGAATGA GAATGCAGCA CAATCTGGAA CAGCAGATTC AAGCTCGAAA CACAACTGGA 
GTCACAGAGG AGGCCCTGAA GGAGTTCAGC ATGATGTTCA AGCACTTTGA CAAGGACAAA 
TCTGGACGTC TTAATCACCA GGAGTTTAAG TCTTGCTTGC GCTCCCTTGG CTATGACCTG 
CCCATGGTTG AGGAAGGAGA GCCTGACCCC GAATTTGAGT CTATTCTTGA CACTGTTGAT 
CCCAACAGGG ATGGCCACGT CTCGCTGCAG GAGTACATGG CGTTCATGAT CAGCAGGGAA 
ACAGAGAACG TGAAATCCAG CGAGGAGATT GAGAGTGCTT TCCGTGCCCT CAGCTCGGAG 
GGGAAGCCTT ACGTGACCAA GGAGGAGCTC TACCAGAACC TGACCCGGGA GCAGGCCGAC 
TACTGCATTT CTCACATGAA GCCCTACATG GATGGCAAGG GCAGAGAACT TCCTTCTGCC 
TACGACTACA TAGAATTTAC ACGTTCACTC TTTGTGAATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015134771.1
CDS	18..7559
Translation	MLSSFRKVKVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSSLRRQKLEDSYRFQFFQRDADELEKWIQEKLQIASDENYKDPSNLQGKLQKHQAFEAEVQANSGAIVKLDETGNQMINEGHFASETIRSFTPYRTAQRPIVTATQVGSHIRTRLQELHRLWELLLEKMREKGVKLLQAQKLVQFLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDLAAHEERVNEVNQFAGKLIQEQHPEEELIKSKQDEVNASWQRLKGLAQQRQGKLFGAAEVQRFNRDVDETISWIKEKGQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPINASQIQVKREELIANWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFRSADESGQALLAAGHYASDEVKEKLTILSDERSALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMDDVATRRDALLSRRNALHERAMKRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYASDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTSVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDADNIKKKQEALVARYEALKDPMVARKQKLADSLRLQQLFRDIEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKIKLNELNQKWDSLKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPTDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTNEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVANDLESEGLMAEEVQAVEHQEVYGMMPRDETDSKTASPWKSARMMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAQRLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKEKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLARGHYASPEIKEKLDILDQERTDLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAVLQSFADQLIAADHYAKGVIANRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIEMGNSLIERGACAGSEDAVKARLAALADQWEFLVQKSSEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRETINGRFQRIKSMAAARRAKLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVDHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCANGEDLIKKNNHHVENITAKMKGLKGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHIQSKAIEVRHASLMKRWNQLLANSAARKKKLLEAQEHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGIAYRRVIRVYQYEVGDDLSGRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFESILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCISHMKPYMDGKGRELPSAYDYIEFTRSLFVN

Target ORF information:

RefSeq Version	XM_015279285.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279285.2

ORF Insert Sequence:

ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG 
TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC 
TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT 
GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT 
GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG 
ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAT CTTTCACGCC TTACAGGACT 
GCACAGCGCC CAATAGTAAC AGCCACCCAA GTTGGGTCCC ACATACGAAC TCGTCTACAG 
GAGCTGCACC GACTATGGGA GTTACTGCTG GAAAAAATGA GAGAGAAGGG AGTGAAGCTG 
TTGCAAGCAC AGAAGCTGGT GCAATTCTTA CGGGAATGTG AAGATGTCAT GGACTGGATC 
AATGACAAGG AAGCAATAGT GACCTCAGAA GAGCTTGGAC AGGACTTAGA GCATGTTGAG 
GTTTTGCAGA AGAAATTTGA AGAGTTCCAA ACAGATCTGG CAGCTCATGA GGAGAGAGTG 
AATGAAGTGA ACCAGTTCGC TGGCAAACTT ATCCAGGAAC AGCACCCTGA GGAGGAACTT 
ATAAAGTCCA AACAGGATGA AGTAAATGCA AGCTGGCAGC GTCTTAAGGG GCTTGCCCAA 
CAGAGGCAAG GAAAACTCTT TGGGGCAGCT GAAGTTCAGC GCTTCAACAG GGATGTGGAT 
GAAACTATCA GCTGGATTAA GGAGAAAGGG CAGTTGATGG CCTCAGATGA CTTTGGCAGA 
GACTTGGCCA GCGTGCAAGC TTTACTACGT AAGCATGAAG GCCTGGAAAG AGATCTTGCA 
GCTTTGGAGG ATAAGGTTAA GGCCCTCTGT GCAGAAGCTG ACCGTTTGCA GCAGTCTCAC 
CCTATAAATG CTTCTCAAAT TCAAGTGAAA CGGGAGGAAC TGATTGCCAA CTGGGAGCAG 
ATCCGAACTC TGGCAGCAGA GAGGCATGCT CGCCTTAATG ACTCCTACAG GTTGCAGCGC 
TTTCTTGCAG ATTTCCGGGA CCTCACTAGC TGGGTAACTG AGATGAAGGC TCTGATAAAT 
GCTGATGAGC TTGCCAATGA TGTGGCTGGA GCAGAAGCCC TTCTAGATAG ACATCAGGAA 
CATAAGGGAG AAATTGATGC TCATGAGGAT AGCTTCAGAT CTGCTGATGA GTCTGGGCAG 
GCTTTGCTTG CAGCTGGGCA CTATGCTTCT GATGAAGTTA AAGAAAAGCT GACTATCCTC 
TCAGATGAAA GATCAGCCTT GCTGGAACTA TGGGAGCTTC GCAGGCAACA GTATGAGCAG 
TGCATGGATC TGCAGCTTTT CTACAGAGAT ACTGAACAAG TTGACAACTG GATGAGCAAA 
CAAGAAGCTT TTCTGCTGAA CGAAGACCTT GGTGATTCTC TGGATAGCGT GGAGGCTCTT 
CTAAAGAAGC ATGAAGATTT TGAGAAATCC CTAAGTGCTC AAGAGGAGAA GATCACAGCA 
CTGGATGAGT TTGCTACTAA ACTGATTCAA AATAACCATT ATGCCATGGA TGACGTTGCT 
ACGCGCAGAG ATGCTCTTCT GAGTCGCCGA AATGCTCTTC ATGAAAGAGC CATGAAGCGC 
CGTGCCCAAC TGGCAGACTC TTTCCATCTC CAGCAGTTTT TCAGGGACTC TGATGAACTG 
AAGAGTTGGG TTAATGAAAA AATGAAAACT GCTACCGATG AGGCTTACAA GGATCCATCC 
AACTTGCAAG GTAAAGTTCA GAAGCATCAG GCTTTTGAAG CAGAGCTTTC TGCTAATCAG 
AGTCGTATTG ATGCACTGGA GAAAGCTGGC CAGAAGTTGA TTGATGTCAA TCACTATGCA 
TCTGATGAGG TGGCAGCTCG CATGAATGAA GTCATCAGCT TGTGGAAGAA ACTTCTGGAA 
GCCACCGAGC TCAAAGGTAT AAAGCTGCGT GAAGCCAATC AACAGCAGCA ATTTAATCGC 
AATGTAGAAG ACATTGAGTT GTGGCTGTAT GAAGTGGAGG GTCACTTGGC TTCTGATGAT 
TATGGAAAAG ATCTTACCAG CGTTCAGAAT CTTCAGAAGA AACATGCCCT GCTAGAGGCA 
GATGTTGCTG CCCATCAGGA TCGGATAGAT GGCATTACCA TCCAGGCACG CCAGTTCCAA 
GATGCTGGGC ACTTTGATGC TGACAATATC AAGAAGAAAC AAGAAGCTTT AGTAGCTCGT 
TATGAAGCTC TGAAGGATCC TATGGTGGCT CGCAAGCAGA AACTTGCAGA TTCTCTTCGC 
CTGCAGCAGC TTTTCCGTGA CATCGAGGAT GAAGAGACCT GGATCAGGGA AAAGGAACCT 
ATTGCAGCCT CAACAAACCG AGGCAAGGAC TTAATTGGTG TCCAGAATCT GCTAAAGAAG 
CACCAGGCTT TGCAGGCAGA AATTGCAGGC CATGAGCCTC GCATTAAAGC AGTCACACAA 
AAAGGAAATG CGATGGTGGA AGAGGGACAT TTTGCTGCTG AAGATGTGAA GATCAAATTG 
AATGAACTAA ACCAAAAATG GGACTCTCTG AAAGCCAAGG CATCTCAACG GCGACAGGAT 
CTAGAGGATT CTCTGCAAGC TCAGCAGTAT TTTGCTGATG CTAATGAGGC AGAATCATGG 
ATGAGGGAAA AGGAGCCCAT TGTAGGCAGC ACGGATTATG GAAAGGATGA AGACTCTGCT 
GAGGCTCTCC TGAAGAAGCA TGAAGCTTTG ATGTCTGATC TTTCTGCTTA CGGCAGTAGC 
ATACAGGCAT TAAGGGAACA GGCCCAGTCC TGCAGGCAAC AAGTTGCTCC CACTGATGAT 
GAAACTGGAA AAGAGCTTGT GCTAGCACTC TATGATTACC AAGAGAAGAG TCCTCGGGAG 
GTGACTATGA AGAAAGGAGA TATTCTAACC CTGCTCAACA GCACCAACAA GGACTGGTGG 
AAGGTTGAAG TTAACGATCG TCAGGGCTTT GTACCAGCTG CCTATGTGAA AAAACTAGAT 
CCTGCCCAGT CTGCATCCCG AGAGAATCTT CTAGAAGAGC AAGGCAGCAT AGCATTGCGA 
CAGGAGCAAA TTGACAACCA GTATCATTCC CTTCTTGAGC TGGGAGAAAA ACGTAAAGGC 
ATGCTAGAAA AAAGCTGCAA GAAGTTTATG CTTTTCCGTG AGGCTAATGA GCTTCAACAG 
TGGATCAATG AGAAGGAAGC TGCGCTCACT AATGAGGAAG TGGGTGCTGA TTTGGAGCAG 
GTGGAGGTGC TACAGAAGAA GTTTGATGAT TTTCAGAAGG ATTTAAAAGC TAATGAGTCA 
CGACTGAAGG ACATAAACAA GGTTGCAAAT GACCTGGAGT CTGAAGGGCT GATGGCAGAG 
GAAGTGCAAG CAGTAGAGCA CCAGGAAGTC TATGGAATGA TGCCCAGAGA TGAAACTGAT 
TCTAAGACAG CCTCTCCTTG GAAGTCTGCA CGTATGATGG TACACACAGT GGCAACGTTT 
AACTCAATCA AGGAACTGAA TGAACGCTGG AGATCCCTGC AGCAGTTGGC AGAGGAGCGA 
AGCCAGCTGT TGGGCAGCGC TCATGAAGTC CAGAGGTTCC ACAGAGATGC TGATGAAACA 
AAAGAATGGA TAGAAGAGAA GAATCAAGCA TTAAATACCG ACAACTATGG ACATGACTTG 
GCCAGTGTGC AGGCTCTGCA GCGCAAGCAT GAAGGCTTTG AGAGAGACTT GGCAGCTCTT 
GGAGACAAGG TGAATTCTCT TGGTGAAACT GCCCAGCGTC TGATCCAGTC ACATCCAGAA 
TCAGCTGAAG ATCTTCAAGA AAAATGTACT GAGTTGAACC AAGCCTGGAG TAGTCTGGGA 
AAACGTGCTG ACCAACGCAA AGAGAAGCTT GGAGATTCTC ATGACCTGCA GCGTTTCCTC 
AGTGATTTTA GAGACCTTAT GTCTTGGATC AATGGAATCC GGGGTCTGGT CTCCTCAGAT 
GAACTTGCAA AAGATGTGAC TGGAGCTGAA GCTCTGTTGG AAAGGCATCA GGAGCACCGT 
ACTGAAATAG ATGCACGGGC TGGCACTTTT CAGGCATTTG AACAGTTTGG ACAGCAGCTT 
CTGGCTCGTG GACACTATGC CAGCCCAGAG ATTAAGGAGA AACTGGATAT TCTAGATCAA 
GAACGGACAG ACCTAGAGAA GGCCTGGGTC CAGCGCAGAA TGATGCTAGA CCAGTGCTTA 
GAACTACAGC TGTTTCATCG GGATTGTGAA CAAGCTGAAA ACTGGATGGC TGCCCGAGAG 
GCGTTCCTAA ATACAGAAGA CAAAGGAGAC TCCTTAGACA GCGTGGAGGC ACTCATCAAG 
AAGCATGAAG ATTTCGATAA AGCAATCAAT GTCCAGGAAG AGAAAATTGC TGTCTTGCAG 
TCTTTTGCTG ACCAACTGAT TGCTGCTGAT CATTATGCAA AAGGAGTCAT TGCTAACAGA 
CGCAATGAGG TTCTGGACAG GTGGCGTCGT CTGAAGGCTC AGATGATTGA GAAGAGATCT 
AAGCTGGGAG AATCTCAGAC CCTCCAACAG TTCAGTCGTG ATGTTGATGA AATAGAAGCT 
TGGATCAGTG AAAAGCTTCA AACTGCAAGT GATGAGTCAT ATAAGGATCC CACAAACATC 
CAGCTTTCCA AACTGCTGAG CAAGCACCAG AAGCACCAAG CCTTTGAAGC TGAGCTCCAC 
GCCAACGCAG ATCGGATTCG TGGAGTCATT GAAATGGGGA ACTCTCTTAT TGAAAGAGGA 
GCGTGTGCTG GCAGCGAGGA TGCTGTGAAG GCTCGGCTGG CTGCCCTGGC TGACCAATGG 
GAGTTCCTGG TCCAGAAGTC ATCAGAGAAG AGTCAGAAAC TGAAAGAAGC AAATAAACAG 
CAGAATTTCA ATACCGGCAT CAAGGACTTT GATTTCTGGC TTTCAGAGGT GGAAGCTTTG 
TTGGCATCTG AAGACTATGG GAAGGACTTG GCATCAGTGA ACAACCTTCT GAAGAAGCAC 
CAATTACTGG AAGCTGATAT ATCTGCTCAT GAGGATCGGC TGAAGGACCT GAACAGCCAG 
GCTGACAGTT TGATGACCAG CAGTGCTTTC GATACCTCCC AAGTAAAGGA TAAACGTGAA 
ACTATAAATG GGCGCTTCCA GAGAATCAAG AGCATGGCAG CTGCCCGCCG TGCGAAGCTC 
AACGAGTCGC ACCGCTTGCA TCAGTTCTTC CGTGACATGG ATGATGAGGA GTCCTGGATC 
AAAGAGAAGA AACTGTTGGT GAGCTCAGAG GACTATGGCA GAGACCTGAC TGGTGTGCAG 
AACCTGAGGA AGAAACATAA GCGCTTGGAA GCAGAATTAG CTGCCCATGA ACCTGCTATC 
CAGGGTGTTC TAGACACTGG CAAGAAGCTT TCAGATGATA ACACAATTGG GAAGGAGGAG 
ATACAGCAAA GACTGGCTCA GTTTGTGGAC CACTGGAAAG AGTTAAAACA GCTGGCAGCT 
GCTCGGGGGC AGCGTCTGGA GGAGTCCCTG GAGTACCAAC AGTTTGTAGC AAATGTTGAG 
GAAGAGGAGG CATGGATCAA CGAGAAAATG ACATTGGTAG CCAGTGAGGA TTATGGAGAC 
ACACTTGCTG CTATCCAGGG CTTGCTGAAG AAGCATGAAG CATTCGAGAC TGACTTTACT 
GTCCACAAAG ACAGAGTGAA TGATGTTTGC GCTAATGGAG AGGATCTCAT TAAAAAGAAT 
AATCACCATG TGGAGAACAT TACTGCTAAG ATGAAGGGCC TCAAGGGCAA GGTATCAGAT 
CTGGAGAAAG CCGCAGCTCA GAGGAAAGCC AAACTGGATG AGAACTCTGC CTTCCTCCAG 
TTCAACTGGA AAGCAGATGT GGTGGAGTCA TGGATAGGTG AGAAGGAAAA CAGTCTGAAG 
ACAGATGATT ATGGCCGTGA CCTCTCCTCT GTGCAAACAC TACTCACCAA ACAGGAAACC 
TTTGATGCTG GACTTCAGGC TTTCCAGCAG GAGGGAATTG CAAACATCAC TGCTCTGAAA 
GACCAGCTAC TGGCAGCCAA ACATATCCAA TCAAAGGCCA TTGAGGTTCG TCACGCTTCC 
TTGATGAAAC GCTGGAATCA GCTGCTTGCT AATTCTGCAG CCAGGAAAAA GAAACTCTTG 
GAGGCTCAGG AGCACTTCAG AAAGGTTGAA GATCTCTTCT TGACTTTTGC CAAGAAGGCC 
TCTGCCTTCA ACAGTTGGTT TGAGAATGCT GAGGAGGACC TGACGGATCC CGTGCGCTGC 
AATTCCCTGG AAGAAATCAA AGCACTGCGA GAAGCCCACG ATGCGTTCCG TTCCTCACTT 
AGCTCTGCCC AAGCTGACTT CAACCAGCTG GCAGAGCTTG ATCGGCAGAT CAAGAGCTTC 
CGTGTAGCCT CCAACCCGTA CACTTGGTTT ACTATGGAGG CTCTTGAAGA AACTTGGAGG 
AATCTGCAGA AAATTATCAA GGAGCGTGAA TTGGAGTTGC AGAAGGAGCA GCGGAGGCAG 
GAAGAAAATG ACAAGTTGCG CCAGGAGTTT GCTCAGCATG CTAATGCCTT CCACCAGTGG 
ATTCAGGAGA CAAGGACTTA CCTGCTAGAT GGCATAGCCT ATCGTCGTGT CATTCGTGTC 
TATCAGTATG AAGTTGGGGA TGATCTATCT GGAAGGTCCT GCATGGTGGA GGAGTCGGGA 
ACACTGGAAT CCCAGCTGGA AGCTACTAAA CGCAAGCACC AGGAGATCCG GGCTATGAGG 
AGCCAGCTGA AGAAGATTGA GGACCTTGGA GCAGCCATGG AAGAGGCACT TATCTTGGAC 
AACAAATACA CAGAGCACAG CACCGTGGGG CTGGCGCAGC AGTGGGACCA GCTTGACCAG 
CTGGGAATGA GAATGCAGCA CAATCTGGAA CAGCAGATTC AAGCTCGAAA CACAACTGGA 
GTCACAGAGG AGGCCCTGAA GGAGTTCAGC ATGATGTTCA AGCACTTTGA CAAGGACAAA 
TCTGGACGTC TTAATCACCA GGAGTTTAAG TCTTGCTTGC GCTCCCTTGG CTATGACCTG 
CCCATGGTTG AGGAAGGAGA GCCTGACCCC GAATTTGAGT CTATTCTTGA CACTGTTGAT 
CCCAACAGGG ATGGCCACGT CTCGCTGCAG GAGTACATGG CGTTCATGAT CAGCAGGGAA 
ACAGAGAACG TGAAATCCAG CGAGGAGATT GAGAGTGCTT TCCGTGCCCT CAGCTCGGAG 
GGGAAGCCTT ACGTGACCAA GGAGGAGCTC TACCAGAACC TGACCCGGGA GCAGGCCGAC 
TACTGCATTT CTCACATGAA GCCCTACATG GATGGCAAGG GCAGAGAACT TCCTTCTGCC 
TACGACTACA TAGAATTTAC ACGTTCACTC TTTGTGAATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_015279285.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015134771.1 Gallus gallus spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X4, mRNA.

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CloneID	OGa43311
Clone ID Related Accession (Same CDS sequence)	XM_015279285.2 , XM_015279285.1
Accession Version	XM_015279285.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7542bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG 
TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC 
TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT 
GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT 
GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG 
ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAT CTTTCACGCC TTACAGGACT 
GCACAGCGCC CAATAGTAAC AGCCACCCAA GTTGGGTCCC ACATACGAAC TCGTCTACAG 
GAGCTGCACC GACTATGGGA GTTACTGCTG GAAAAAATGA GAGAGAAGGG AGTGAAGCTG 
TTGCAAGCAC AGAAGCTGGT GCAATTCTTA CGGGAATGTG AAGATGTCAT GGACTGGATC 
AATGACAAGG AAGCAATAGT GACCTCAGAA GAGCTTGGAC AGGACTTAGA GCATGTTGAG 
GTTTTGCAGA AGAAATTTGA AGAGTTCCAA ACAGATCTGG CAGCTCATGA GGAGAGAGTG 
AATGAAGTGA ACCAGTTCGC TGGCAAACTT ATCCAGGAAC AGCACCCTGA GGAGGAACTT 
ATAAAGTCCA AACAGGATGA AGTAAATGCA AGCTGGCAGC GTCTTAAGGG GCTTGCCCAA 
CAGAGGCAAG GAAAACTCTT TGGGGCAGCT GAAGTTCAGC GCTTCAACAG GGATGTGGAT 
GAAACTATCA GCTGGATTAA GGAGAAAGGG CAGTTGATGG CCTCAGATGA CTTTGGCAGA 
GACTTGGCCA GCGTGCAAGC TTTACTACGT AAGCATGAAG GCCTGGAAAG AGATCTTGCA 
GCTTTGGAGG ATAAGGTTAA GGCCCTCTGT GCAGAAGCTG ACCGTTTGCA GCAGTCTCAC 
CCTATAAATG CTTCTCAAAT TCAAGTGAAA CGGGAGGAAC TGATTGCCAA CTGGGAGCAG 
ATCCGAACTC TGGCAGCAGA GAGGCATGCT CGCCTTAATG ACTCCTACAG GTTGCAGCGC 
TTTCTTGCAG ATTTCCGGGA CCTCACTAGC TGGGTAACTG AGATGAAGGC TCTGATAAAT 
GCTGATGAGC TTGCCAATGA TGTGGCTGGA GCAGAAGCCC TTCTAGATAG ACATCAGGAA 
CATAAGGGAG AAATTGATGC TCATGAGGAT AGCTTCAGAT CTGCTGATGA GTCTGGGCAG 
GCTTTGCTTG CAGCTGGGCA CTATGCTTCT GATGAAGTTA AAGAAAAGCT GACTATCCTC 
TCAGATGAAA GATCAGCCTT GCTGGAACTA TGGGAGCTTC GCAGGCAACA GTATGAGCAG 
TGCATGGATC TGCAGCTTTT CTACAGAGAT ACTGAACAAG TTGACAACTG GATGAGCAAA 
CAAGAAGCTT TTCTGCTGAA CGAAGACCTT GGTGATTCTC TGGATAGCGT GGAGGCTCTT 
CTAAAGAAGC ATGAAGATTT TGAGAAATCC CTAAGTGCTC AAGAGGAGAA GATCACAGCA 
CTGGATGAGT TTGCTACTAA ACTGATTCAA AATAACCATT ATGCCATGGA TGACGTTGCT 
ACGCGCAGAG ATGCTCTTCT GAGTCGCCGA AATGCTCTTC ATGAAAGAGC CATGAAGCGC 
CGTGCCCAAC TGGCAGACTC TTTCCATCTC CAGCAGTTTT TCAGGGACTC TGATGAACTG 
AAGAGTTGGG TTAATGAAAA AATGAAAACT GCTACCGATG AGGCTTACAA GGATCCATCC 
AACTTGCAAG GTAAAGTTCA GAAGCATCAG GCTTTTGAAG CAGAGCTTTC TGCTAATCAG 
AGTCGTATTG ATGCACTGGA GAAAGCTGGC CAGAAGTTGA TTGATGTCAA TCACTATGCA 
TCTGATGAGG TGGCAGCTCG CATGAATGAA GTCATCAGCT TGTGGAAGAA ACTTCTGGAA 
GCCACCGAGC TCAAAGGTAT AAAGCTGCGT GAAGCCAATC AACAGCAGCA ATTTAATCGC 
AATGTAGAAG ACATTGAGTT GTGGCTGTAT GAAGTGGAGG GTCACTTGGC TTCTGATGAT 
TATGGAAAAG ATCTTACCAG CGTTCAGAAT CTTCAGAAGA AACATGCCCT GCTAGAGGCA 
GATGTTGCTG CCCATCAGGA TCGGATAGAT GGCATTACCA TCCAGGCACG CCAGTTCCAA 
GATGCTGGGC ACTTTGATGC TGACAATATC AAGAAGAAAC AAGAAGCTTT AGTAGCTCGT 
TATGAAGCTC TGAAGGATCC TATGGTGGCT CGCAAGCAGA AACTTGCAGA TTCTCTTCGC 
CTGCAGCAGC TTTTCCGTGA CATCGAGGAT GAAGAGACCT GGATCAGGGA AAAGGAACCT 
ATTGCAGCCT CAACAAACCG AGGCAAGGAC TTAATTGGTG TCCAGAATCT GCTAAAGAAG 
CACCAGGCTT TGCAGGCAGA AATTGCAGGC CATGAGCCTC GCATTAAAGC AGTCACACAA 
AAAGGAAATG CGATGGTGGA AGAGGGACAT TTTGCTGCTG AAGATGTGAA GATCAAATTG 
AATGAACTAA ACCAAAAATG GGACTCTCTG AAAGCCAAGG CATCTCAACG GCGACAGGAT 
CTAGAGGATT CTCTGCAAGC TCAGCAGTAT TTTGCTGATG CTAATGAGGC AGAATCATGG 
ATGAGGGAAA AGGAGCCCAT TGTAGGCAGC ACGGATTATG GAAAGGATGA AGACTCTGCT 
GAGGCTCTCC TGAAGAAGCA TGAAGCTTTG ATGTCTGATC TTTCTGCTTA CGGCAGTAGC 
ATACAGGCAT TAAGGGAACA GGCCCAGTCC TGCAGGCAAC AAGTTGCTCC CACTGATGAT 
GAAACTGGAA AAGAGCTTGT GCTAGCACTC TATGATTACC AAGAGAAGAG TCCTCGGGAG 
GTGACTATGA AGAAAGGAGA TATTCTAACC CTGCTCAACA GCACCAACAA GGACTGGTGG 
AAGGTTGAAG TTAACGATCG TCAGGGCTTT GTACCAGCTG CCTATGTGAA AAAACTAGAT 
CCTGCCCAGT CTGCATCCCG AGAGAATCTT CTAGAAGAGC AAGGCAGCAT AGCATTGCGA 
CAGGAGCAAA TTGACAACCA GTATCATTCC CTTCTTGAGC TGGGAGAAAA ACGTAAAGGC 
ATGCTAGAAA AAAGCTGCAA GAAGTTTATG CTTTTCCGTG AGGCTAATGA GCTTCAACAG 
TGGATCAATG AGAAGGAAGC TGCGCTCACT AATGAGGAAG TGGGTGCTGA TTTGGAGCAG 
GTGGAGGTGC TACAGAAGAA GTTTGATGAT TTTCAGAAGG ATTTAAAAGC TAATGAGTCA 
CGACTGAAGG ACATAAACAA GGTTGCAAAT GACCTGGAGT CTGAAGGGCT GATGGCAGAG 
GAAGTGCAAG CAGTAGAGCA CCAGGAAGTC TATGGAATGA TGCCCAGAGA TGAAACTGAT 
TCTAAGACAG CCTCTCCTTG GAAGTCTGCA CGTATGATGG TACACACAGT GGCAACGTTT 
AACTCAATCA AGGAACTGAA TGAACGCTGG AGATCCCTGC AGCAGTTGGC AGAGGAGCGA 
AGCCAGCTGT TGGGCAGCGC TCATGAAGTC CAGAGGTTCC ACAGAGATGC TGATGAAACA 
AAAGAATGGA TAGAAGAGAA GAATCAAGCA TTAAATACCG ACAACTATGG ACATGACTTG 
GCCAGTGTGC AGGCTCTGCA GCGCAAGCAT GAAGGCTTTG AGAGAGACTT GGCAGCTCTT 
GGAGACAAGG TGAATTCTCT TGGTGAAACT GCCCAGCGTC TGATCCAGTC ACATCCAGAA 
TCAGCTGAAG ATCTTCAAGA AAAATGTACT GAGTTGAACC AAGCCTGGAG TAGTCTGGGA 
AAACGTGCTG ACCAACGCAA AGAGAAGCTT GGAGATTCTC ATGACCTGCA GCGTTTCCTC 
AGTGATTTTA GAGACCTTAT GTCTTGGATC AATGGAATCC GGGGTCTGGT CTCCTCAGAT 
GAACTTGCAA AAGATGTGAC TGGAGCTGAA GCTCTGTTGG AAAGGCATCA GGAGCACCGT 
ACTGAAATAG ATGCACGGGC TGGCACTTTT CAGGCATTTG AACAGTTTGG ACAGCAGCTT 
CTGGCTCGTG GACACTATGC CAGCCCAGAG ATTAAGGAGA AACTGGATAT TCTAGATCAA 
GAACGGACAG ACCTAGAGAA GGCCTGGGTC CAGCGCAGAA TGATGCTAGA CCAGTGCTTA 
GAACTACAGC TGTTTCATCG GGATTGTGAA CAAGCTGAAA ACTGGATGGC TGCCCGAGAG 
GCGTTCCTAA ATACAGAAGA CAAAGGAGAC TCCTTAGACA GCGTGGAGGC ACTCATCAAG 
AAGCATGAAG ATTTCGATAA AGCAATCAAT GTCCAGGAAG AGAAAATTGC TGTCTTGCAG 
TCTTTTGCTG ACCAACTGAT TGCTGCTGAT CATTATGCAA AAGGAGTCAT TGCTAACAGA 
CGCAATGAGG TTCTGGACAG GTGGCGTCGT CTGAAGGCTC AGATGATTGA GAAGAGATCT 
AAGCTGGGAG AATCTCAGAC CCTCCAACAG TTCAGTCGTG ATGTTGATGA AATAGAAGCT 
TGGATCAGTG AAAAGCTTCA AACTGCAAGT GATGAGTCAT ATAAGGATCC CACAAACATC 
CAGCTTTCCA AACTGCTGAG CAAGCACCAG AAGCACCAAG CCTTTGAAGC TGAGCTCCAC 
GCCAACGCAG ATCGGATTCG TGGAGTCATT GAAATGGGGA ACTCTCTTAT TGAAAGAGGA 
GCGTGTGCTG GCAGCGAGGA TGCTGTGAAG GCTCGGCTGG CTGCCCTGGC TGACCAATGG 
GAGTTCCTGG TCCAGAAGTC ATCAGAGAAG AGTCAGAAAC TGAAAGAAGC AAATAAACAG 
CAGAATTTCA ATACCGGCAT CAAGGACTTT GATTTCTGGC TTTCAGAGGT GGAAGCTTTG 
TTGGCATCTG AAGACTATGG GAAGGACTTG GCATCAGTGA ACAACCTTCT GAAGAAGCAC 
CAATTACTGG AAGCTGATAT ATCTGCTCAT GAGGATCGGC TGAAGGACCT GAACAGCCAG 
GCTGACAGTT TGATGACCAG CAGTGCTTTC GATACCTCCC AAGTAAAGGA TAAACGTGAA 
ACTATAAATG GGCGCTTCCA GAGAATCAAG AGCATGGCAG CTGCCCGCCG TGCGAAGCTC 
AACGAGTCGC ACCGCTTGCA TCAGTTCTTC CGTGACATGG ATGATGAGGA GTCCTGGATC 
AAAGAGAAGA AACTGTTGGT GAGCTCAGAG GACTATGGCA GAGACCTGAC TGGTGTGCAG 
AACCTGAGGA AGAAACATAA GCGCTTGGAA GCAGAATTAG CTGCCCATGA ACCTGCTATC 
CAGGGTGTTC TAGACACTGG CAAGAAGCTT TCAGATGATA ACACAATTGG GAAGGAGGAG 
ATACAGCAAA GACTGGCTCA GTTTGTGGAC CACTGGAAAG AGTTAAAACA GCTGGCAGCT 
GCTCGGGGGC AGCGTCTGGA GGAGTCCCTG GAGTACCAAC AGTTTGTAGC AAATGTTGAG 
GAAGAGGAGG CATGGATCAA CGAGAAAATG ACATTGGTAG CCAGTGAGGA TTATGGAGAC 
ACACTTGCTG CTATCCAGGG CTTGCTGAAG AAGCATGAAG CATTCGAGAC TGACTTTACT 
GTCCACAAAG ACAGAGTGAA TGATGTTTGC GCTAATGGAG AGGATCTCAT TAAAAAGAAT 
AATCACCATG TGGAGAACAT TACTGCTAAG ATGAAGGGCC TCAAGGGCAA GGTATCAGAT 
CTGGAGAAAG CCGCAGCTCA GAGGAAAGCC AAACTGGATG AGAACTCTGC CTTCCTCCAG 
TTCAACTGGA AAGCAGATGT GGTGGAGTCA TGGATAGGTG AGAAGGAAAA CAGTCTGAAG 
ACAGATGATT ATGGCCGTGA CCTCTCCTCT GTGCAAACAC TACTCACCAA ACAGGAAACC 
TTTGATGCTG GACTTCAGGC TTTCCAGCAG GAGGGAATTG CAAACATCAC TGCTCTGAAA 
GACCAGCTAC TGGCAGCCAA ACATATCCAA TCAAAGGCCA TTGAGGTTCG TCACGCTTCC 
TTGATGAAAC GCTGGAATCA GCTGCTTGCT AATTCTGCAG CCAGGAAAAA GAAACTCTTG 
GAGGCTCAGG AGCACTTCAG AAAGGTTGAA GATCTCTTCT TGACTTTTGC CAAGAAGGCC 
TCTGCCTTCA ACAGTTGGTT TGAGAATGCT GAGGAGGACC TGACGGATCC CGTGCGCTGC 
AATTCCCTGG AAGAAATCAA AGCACTGCGA GAAGCCCACG ATGCGTTCCG TTCCTCACTT 
AGCTCTGCCC AAGCTGACTT CAACCAGCTG GCAGAGCTTG ATCGGCAGAT CAAGAGCTTC 
CGTGTAGCCT CCAACCCGTA CACTTGGTTT ACTATGGAGG CTCTTGAAGA AACTTGGAGG 
AATCTGCAGA AAATTATCAA GGAGCGTGAA TTGGAGTTGC AGAAGGAGCA GCGGAGGCAG 
GAAGAAAATG ACAAGTTGCG CCAGGAGTTT GCTCAGCATG CTAATGCCTT CCACCAGTGG 
ATTCAGGAGA CAAGGACTTA CCTGCTAGAT GGCATAGCCT ATCGTCGTGT CATTCGTGTC 
TATCAGTATG AAGTTGGGGA TGATCTATCT GGAAGGTCCT GCATGGTGGA GGAGTCGGGA 
ACACTGGAAT CCCAGCTGGA AGCTACTAAA CGCAAGCACC AGGAGATCCG GGCTATGAGG 
AGCCAGCTGA AGAAGATTGA GGACCTTGGA GCAGCCATGG AAGAGGCACT TATCTTGGAC 
AACAAATACA CAGAGCACAG CACCGTGGGG CTGGCGCAGC AGTGGGACCA GCTTGACCAG 
CTGGGAATGA GAATGCAGCA CAATCTGGAA CAGCAGATTC AAGCTCGAAA CACAACTGGA 
GTCACAGAGG AGGCCCTGAA GGAGTTCAGC ATGATGTTCA AGCACTTTGA CAAGGACAAA 
TCTGGACGTC TTAATCACCA GGAGTTTAAG TCTTGCTTGC GCTCCCTTGG CTATGACCTG 
CCCATGGTTG AGGAAGGAGA GCCTGACCCC GAATTTGAGT CTATTCTTGA CACTGTTGAT 
CCCAACAGGG ATGGCCACGT CTCGCTGCAG GAGTACATGG CGTTCATGAT CAGCAGGGAA 
ACAGAGAACG TGAAATCCAG CGAGGAGATT GAGAGTGCTT TCCGTGCCCT CAGCTCGGAG 
GGGAAGCCTT ACGTGACCAA GGAGGAGCTC TACCAGAACC TGACCCGGGA GCAGGCCGAC 
TACTGCATTT CTCACATGAA GCCCTACATG GATGGCAAGG GCAGAGAACT TCCTTCTGCC 
TACGACTACA TAGAATTTAC ACGTTCACTC TTTGTGAATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015134771.1
CDS	17..7558
Translation	MLSSFRKVKVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSSLRRQKLEDSYRFQFFQRDADELEKWIQEKLQIASDENYKDPSNLQGKLQKHQAFEAEVQANSGAIVKLDETGNQMINEGHFASETIRSFTPYRTAQRPIVTATQVGSHIRTRLQELHRLWELLLEKMREKGVKLLQAQKLVQFLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDLAAHEERVNEVNQFAGKLIQEQHPEEELIKSKQDEVNASWQRLKGLAQQRQGKLFGAAEVQRFNRDVDETISWIKEKGQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPINASQIQVKREELIANWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFRSADESGQALLAAGHYASDEVKEKLTILSDERSALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMDDVATRRDALLSRRNALHERAMKRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYASDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTSVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDADNIKKKQEALVARYEALKDPMVARKQKLADSLRLQQLFRDIEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKIKLNELNQKWDSLKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPTDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTNEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVANDLESEGLMAEEVQAVEHQEVYGMMPRDETDSKTASPWKSARMMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAQRLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKEKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLARGHYASPEIKEKLDILDQERTDLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAVLQSFADQLIAADHYAKGVIANRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIEMGNSLIERGACAGSEDAVKARLAALADQWEFLVQKSSEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRETINGRFQRIKSMAAARRAKLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVDHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCANGEDLIKKNNHHVENITAKMKGLKGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHIQSKAIEVRHASLMKRWNQLLANSAARKKKLLEAQEHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGIAYRRVIRVYQYEVGDDLSGRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFESILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCISHMKPYMDGKGRELPSAYDYIEFTRSLFVN

Target ORF information:

RefSeq Version	XM_015279285.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279285.1

ORF Insert Sequence:

ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG 
TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC 
TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT 
GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT 
GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG 
ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAT CTTTCACGCC TTACAGGACT 
GCACAGCGCC CAATAGTAAC AGCCACCCAA GTTGGGTCCC ACATACGAAC TCGTCTACAG 
GAGCTGCACC GACTATGGGA GTTACTGCTG GAAAAAATGA GAGAGAAGGG AGTGAAGCTG 
TTGCAAGCAC AGAAGCTGGT GCAATTCTTA CGGGAATGTG AAGATGTCAT GGACTGGATC 
AATGACAAGG AAGCAATAGT GACCTCAGAA GAGCTTGGAC AGGACTTAGA GCATGTTGAG 
GTTTTGCAGA AGAAATTTGA AGAGTTCCAA ACAGATCTGG CAGCTCATGA GGAGAGAGTG 
AATGAAGTGA ACCAGTTCGC TGGCAAACTT ATCCAGGAAC AGCACCCTGA GGAGGAACTT 
ATAAAGTCCA AACAGGATGA AGTAAATGCA AGCTGGCAGC GTCTTAAGGG GCTTGCCCAA 
CAGAGGCAAG GAAAACTCTT TGGGGCAGCT GAAGTTCAGC GCTTCAACAG GGATGTGGAT 
GAAACTATCA GCTGGATTAA GGAGAAAGGG CAGTTGATGG CCTCAGATGA CTTTGGCAGA 
GACTTGGCCA GCGTGCAAGC TTTACTACGT AAGCATGAAG GCCTGGAAAG AGATCTTGCA 
GCTTTGGAGG ATAAGGTTAA GGCCCTCTGT GCAGAAGCTG ACCGTTTGCA GCAGTCTCAC 
CCTATAAATG CTTCTCAAAT TCAAGTGAAA CGGGAGGAAC TGATTGCCAA CTGGGAGCAG 
ATCCGAACTC TGGCAGCAGA GAGGCATGCT CGCCTTAATG ACTCCTACAG GTTGCAGCGC 
TTTCTTGCAG ATTTCCGGGA CCTCACTAGC TGGGTAACTG AGATGAAGGC TCTGATAAAT 
GCTGATGAGC TTGCCAATGA TGTGGCTGGA GCAGAAGCCC TTCTAGATAG ACATCAGGAA 
CATAAGGGAG AAATTGATGC TCATGAGGAT AGCTTCAGAT CTGCTGATGA GTCTGGGCAG 
GCTTTGCTTG CAGCTGGGCA CTATGCTTCT GATGAAGTTA AAGAAAAGCT GACTATCCTC 
TCAGATGAAA GATCAGCCTT GCTGGAACTA TGGGAGCTTC GCAGGCAACA GTATGAGCAG 
TGCATGGATC TGCAGCTTTT CTACAGAGAT ACTGAACAAG TTGACAACTG GATGAGCAAA 
CAAGAAGCTT TTCTGCTGAA CGAAGACCTT GGTGATTCTC TGGATAGCGT GGAGGCTCTT 
CTAAAGAAGC ATGAAGATTT TGAGAAATCC CTAAGTGCTC AAGAGGAGAA GATCACAGCA 
CTGGATGAGT TTGCTACTAA ACTGATTCAA AATAACCATT ATGCCATGGA TGACGTTGCT 
ACGCGCAGAG ATGCTCTTCT GAGTCGCCGA AATGCTCTTC ATGAAAGAGC CATGAAGCGC 
CGTGCCCAAC TGGCAGACTC TTTCCATCTC CAGCAGTTTT TCAGGGACTC TGATGAACTG 
AAGAGTTGGG TTAATGAAAA AATGAAAACT GCTACCGATG AGGCTTACAA GGATCCATCC 
AACTTGCAAG GTAAAGTTCA GAAGCATCAG GCTTTTGAAG CAGAGCTTTC TGCTAATCAG 
AGTCGTATTG ATGCACTGGA GAAAGCTGGC CAGAAGTTGA TTGATGTCAA TCACTATGCA 
TCTGATGAGG TGGCAGCTCG CATGAATGAA GTCATCAGCT TGTGGAAGAA ACTTCTGGAA 
GCCACCGAGC TCAAAGGTAT AAAGCTGCGT GAAGCCAATC AACAGCAGCA ATTTAATCGC 
AATGTAGAAG ACATTGAGTT GTGGCTGTAT GAAGTGGAGG GTCACTTGGC TTCTGATGAT 
TATGGAAAAG ATCTTACCAG CGTTCAGAAT CTTCAGAAGA AACATGCCCT GCTAGAGGCA 
GATGTTGCTG CCCATCAGGA TCGGATAGAT GGCATTACCA TCCAGGCACG CCAGTTCCAA 
GATGCTGGGC ACTTTGATGC TGACAATATC AAGAAGAAAC AAGAAGCTTT AGTAGCTCGT 
TATGAAGCTC TGAAGGATCC TATGGTGGCT CGCAAGCAGA AACTTGCAGA TTCTCTTCGC 
CTGCAGCAGC TTTTCCGTGA CATCGAGGAT GAAGAGACCT GGATCAGGGA AAAGGAACCT 
ATTGCAGCCT CAACAAACCG AGGCAAGGAC TTAATTGGTG TCCAGAATCT GCTAAAGAAG 
CACCAGGCTT TGCAGGCAGA AATTGCAGGC CATGAGCCTC GCATTAAAGC AGTCACACAA 
AAAGGAAATG CGATGGTGGA AGAGGGACAT TTTGCTGCTG AAGATGTGAA GATCAAATTG 
AATGAACTAA ACCAAAAATG GGACTCTCTG AAAGCCAAGG CATCTCAACG GCGACAGGAT 
CTAGAGGATT CTCTGCAAGC TCAGCAGTAT TTTGCTGATG CTAATGAGGC AGAATCATGG 
ATGAGGGAAA AGGAGCCCAT TGTAGGCAGC ACGGATTATG GAAAGGATGA AGACTCTGCT 
GAGGCTCTCC TGAAGAAGCA TGAAGCTTTG ATGTCTGATC TTTCTGCTTA CGGCAGTAGC 
ATACAGGCAT TAAGGGAACA GGCCCAGTCC TGCAGGCAAC AAGTTGCTCC CACTGATGAT 
GAAACTGGAA AAGAGCTTGT GCTAGCACTC TATGATTACC AAGAGAAGAG TCCTCGGGAG 
GTGACTATGA AGAAAGGAGA TATTCTAACC CTGCTCAACA GCACCAACAA GGACTGGTGG 
AAGGTTGAAG TTAACGATCG TCAGGGCTTT GTACCAGCTG CCTATGTGAA AAAACTAGAT 
CCTGCCCAGT CTGCATCCCG AGAGAATCTT CTAGAAGAGC AAGGCAGCAT AGCATTGCGA 
CAGGAGCAAA TTGACAACCA GTATCATTCC CTTCTTGAGC TGGGAGAAAA ACGTAAAGGC 
ATGCTAGAAA AAAGCTGCAA GAAGTTTATG CTTTTCCGTG AGGCTAATGA GCTTCAACAG 
TGGATCAATG AGAAGGAAGC TGCGCTCACT AATGAGGAAG TGGGTGCTGA TTTGGAGCAG 
GTGGAGGTGC TACAGAAGAA GTTTGATGAT TTTCAGAAGG ATTTAAAAGC TAATGAGTCA 
CGACTGAAGG ACATAAACAA GGTTGCAAAT GACCTGGAGT CTGAAGGGCT GATGGCAGAG 
GAAGTGCAAG CAGTAGAGCA CCAGGAAGTC TATGGAATGA TGCCCAGAGA TGAAACTGAT 
TCTAAGACAG CCTCTCCTTG GAAGTCTGCA CGTATGATGG TACACACAGT GGCAACGTTT 
AACTCAATCA AGGAACTGAA TGAACGCTGG AGATCCCTGC AGCAGTTGGC AGAGGAGCGA 
AGCCAGCTGT TGGGCAGCGC TCATGAAGTC CAGAGGTTCC ACAGAGATGC TGATGAAACA 
AAAGAATGGA TAGAAGAGAA GAATCAAGCA TTAAATACCG ACAACTATGG ACATGACTTG 
GCCAGTGTGC AGGCTCTGCA GCGCAAGCAT GAAGGCTTTG AGAGAGACTT GGCAGCTCTT 
GGAGACAAGG TGAATTCTCT TGGTGAAACT GCCCAGCGTC TGATCCAGTC ACATCCAGAA 
TCAGCTGAAG ATCTTCAAGA AAAATGTACT GAGTTGAACC AAGCCTGGAG TAGTCTGGGA 
AAACGTGCTG ACCAACGCAA AGAGAAGCTT GGAGATTCTC ATGACCTGCA GCGTTTCCTC 
AGTGATTTTA GAGACCTTAT GTCTTGGATC AATGGAATCC GGGGTCTGGT CTCCTCAGAT 
GAACTTGCAA AAGATGTGAC TGGAGCTGAA GCTCTGTTGG AAAGGCATCA GGAGCACCGT 
ACTGAAATAG ATGCACGGGC TGGCACTTTT CAGGCATTTG AACAGTTTGG ACAGCAGCTT 
CTGGCTCGTG GACACTATGC CAGCCCAGAG ATTAAGGAGA AACTGGATAT TCTAGATCAA 
GAACGGACAG ACCTAGAGAA GGCCTGGGTC CAGCGCAGAA TGATGCTAGA CCAGTGCTTA 
GAACTACAGC TGTTTCATCG GGATTGTGAA CAAGCTGAAA ACTGGATGGC TGCCCGAGAG 
GCGTTCCTAA ATACAGAAGA CAAAGGAGAC TCCTTAGACA GCGTGGAGGC ACTCATCAAG 
AAGCATGAAG ATTTCGATAA AGCAATCAAT GTCCAGGAAG AGAAAATTGC TGTCTTGCAG 
TCTTTTGCTG ACCAACTGAT TGCTGCTGAT CATTATGCAA AAGGAGTCAT TGCTAACAGA 
CGCAATGAGG TTCTGGACAG GTGGCGTCGT CTGAAGGCTC AGATGATTGA GAAGAGATCT 
AAGCTGGGAG AATCTCAGAC CCTCCAACAG TTCAGTCGTG ATGTTGATGA AATAGAAGCT 
TGGATCAGTG AAAAGCTTCA AACTGCAAGT GATGAGTCAT ATAAGGATCC CACAAACATC 
CAGCTTTCCA AACTGCTGAG CAAGCACCAG AAGCACCAAG CCTTTGAAGC TGAGCTCCAC 
GCCAACGCAG ATCGGATTCG TGGAGTCATT GAAATGGGGA ACTCTCTTAT TGAAAGAGGA 
GCGTGTGCTG GCAGCGAGGA TGCTGTGAAG GCTCGGCTGG CTGCCCTGGC TGACCAATGG 
GAGTTCCTGG TCCAGAAGTC ATCAGAGAAG AGTCAGAAAC TGAAAGAAGC AAATAAACAG 
CAGAATTTCA ATACCGGCAT CAAGGACTTT GATTTCTGGC TTTCAGAGGT GGAAGCTTTG 
TTGGCATCTG AAGACTATGG GAAGGACTTG GCATCAGTGA ACAACCTTCT GAAGAAGCAC 
CAATTACTGG AAGCTGATAT ATCTGCTCAT GAGGATCGGC TGAAGGACCT GAACAGCCAG 
GCTGACAGTT TGATGACCAG CAGTGCTTTC GATACCTCCC AAGTAAAGGA TAAACGTGAA 
ACTATAAATG GGCGCTTCCA GAGAATCAAG AGCATGGCAG CTGCCCGCCG TGCGAAGCTC 
AACGAGTCGC ACCGCTTGCA TCAGTTCTTC CGTGACATGG ATGATGAGGA GTCCTGGATC 
AAAGAGAAGA AACTGTTGGT GAGCTCAGAG GACTATGGCA GAGACCTGAC TGGTGTGCAG 
AACCTGAGGA AGAAACATAA GCGCTTGGAA GCAGAATTAG CTGCCCATGA ACCTGCTATC 
CAGGGTGTTC TAGACACTGG CAAGAAGCTT TCAGATGATA ACACAATTGG GAAGGAGGAG 
ATACAGCAAA GACTGGCTCA GTTTGTGGAC CACTGGAAAG AGTTAAAACA GCTGGCAGCT 
GCTCGGGGGC AGCGTCTGGA GGAGTCCCTG GAGTACCAAC AGTTTGTAGC AAATGTTGAG 
GAAGAGGAGG CATGGATCAA CGAGAAAATG ACATTGGTAG CCAGTGAGGA TTATGGAGAC 
ACACTTGCTG CTATCCAGGG CTTGCTGAAG AAGCATGAAG CATTCGAGAC TGACTTTACT 
GTCCACAAAG ACAGAGTGAA TGATGTTTGC GCTAATGGAG AGGATCTCAT TAAAAAGAAT 
AATCACCATG TGGAGAACAT TACTGCTAAG ATGAAGGGCC TCAAGGGCAA GGTATCAGAT 
CTGGAGAAAG CCGCAGCTCA GAGGAAAGCC AAACTGGATG AGAACTCTGC CTTCCTCCAG 
TTCAACTGGA AAGCAGATGT GGTGGAGTCA TGGATAGGTG AGAAGGAAAA CAGTCTGAAG 
ACAGATGATT ATGGCCGTGA CCTCTCCTCT GTGCAAACAC TACTCACCAA ACAGGAAACC 
TTTGATGCTG GACTTCAGGC TTTCCAGCAG GAGGGAATTG CAAACATCAC TGCTCTGAAA 
GACCAGCTAC TGGCAGCCAA ACATATCCAA TCAAAGGCCA TTGAGGTTCG TCACGCTTCC 
TTGATGAAAC GCTGGAATCA GCTGCTTGCT AATTCTGCAG CCAGGAAAAA GAAACTCTTG 
GAGGCTCAGG AGCACTTCAG AAAGGTTGAA GATCTCTTCT TGACTTTTGC CAAGAAGGCC 
TCTGCCTTCA ACAGTTGGTT TGAGAATGCT GAGGAGGACC TGACGGATCC CGTGCGCTGC 
AATTCCCTGG AAGAAATCAA AGCACTGCGA GAAGCCCACG ATGCGTTCCG TTCCTCACTT 
AGCTCTGCCC AAGCTGACTT CAACCAGCTG GCAGAGCTTG ATCGGCAGAT CAAGAGCTTC 
CGTGTAGCCT CCAACCCGTA CACTTGGTTT ACTATGGAGG CTCTTGAAGA AACTTGGAGG 
AATCTGCAGA AAATTATCAA GGAGCGTGAA TTGGAGTTGC AGAAGGAGCA GCGGAGGCAG 
GAAGAAAATG ACAAGTTGCG CCAGGAGTTT GCTCAGCATG CTAATGCCTT CCACCAGTGG 
ATTCAGGAGA CAAGGACTTA CCTGCTAGAT GGCATAGCCT ATCGTCGTGT CATTCGTGTC 
TATCAGTATG AAGTTGGGGA TGATCTATCT GGAAGGTCCT GCATGGTGGA GGAGTCGGGA 
ACACTGGAAT CCCAGCTGGA AGCTACTAAA CGCAAGCACC AGGAGATCCG GGCTATGAGG 
AGCCAGCTGA AGAAGATTGA GGACCTTGGA GCAGCCATGG AAGAGGCACT TATCTTGGAC 
AACAAATACA CAGAGCACAG CACCGTGGGG CTGGCGCAGC AGTGGGACCA GCTTGACCAG 
CTGGGAATGA GAATGCAGCA CAATCTGGAA CAGCAGATTC AAGCTCGAAA CACAACTGGA 
GTCACAGAGG AGGCCCTGAA GGAGTTCAGC ATGATGTTCA AGCACTTTGA CAAGGACAAA 
TCTGGACGTC TTAATCACCA GGAGTTTAAG TCTTGCTTGC GCTCCCTTGG CTATGACCTG 
CCCATGGTTG AGGAAGGAGA GCCTGACCCC GAATTTGAGT CTATTCTTGA CACTGTTGAT 
CCCAACAGGG ATGGCCACGT CTCGCTGCAG GAGTACATGG CGTTCATGAT CAGCAGGGAA 
ACAGAGAACG TGAAATCCAG CGAGGAGATT GAGAGTGCTT TCCGTGCCCT CAGCTCGGAG 
GGGAAGCCTT ACGTGACCAA GGAGGAGCTC TACCAGAACC TGACCCGGGA GCAGGCCGAC 
TACTGCATTT CTCACATGAA GCCCTACATG GATGGCAAGG GCAGAGAACT TCCTTCTGCC 
TACGACTACA TAGAATTTAC ACGTTCACTC TTTGTGAATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_015279286.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015134772.1 Gallus gallus spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X5, mRNA.

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CloneID	OGa43312
Clone ID Related Accession (Same CDS sequence)	XM_015279286.1 , XM_015279286.2
Accession Version	XM_015279286.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7539bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG 
TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC 
TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT 
GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT 
GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG 
ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAT CTTTCACGCC TTACAGGACT 
GCACAGCGCC CAATAGTAAC AGCCACCCAA GTTGGGTCCC ACATACGAAC TCGTCTACAG 
GAGCTGCACC GACTATGGGA GTTACTGCTG GAAAAAATGA GAGAGAAGGG AGTGAAGCTG 
TTGCAAGCAC AGAAGCTGGT GCAATTCTTA CGGGAATGTG AAGATGTCAT GGACTGGATC 
AATGACAAGG AAGCAATAGT GACCTCAGAA GAGCTTGGAC AGGACTTAGA GCATGTTGAG 
GTTTTGCAGA AGAAATTTGA AGAGTTCCAA ACAGATCTGG CAGCTCATGA GGAGAGAGTG 
AATGAAGTGA ACCAGTTCGC TGGCAAACTT ATCCAGGAAC AGCACCCTGA GGAGGAACTT 
ATAAAGTCCA AACAGGATGA AGTAAATGCA AGCTGGCAGC GTCTTAAGGG GCTTGCCCAA 
CAGAGGCAAG GAAAACTCTT TGGGGCAGCT GAAGTTCAGC GCTTCAACAG GGATGTGGAT 
GAAACTATCA GCTGGATTAA GGAGAAAGGG CAGTTGATGG CCTCAGATGA CTTTGGCAGA 
GACTTGGCCA GCGTGCAAGC TTTACTACGT AAGCATGAAG GCCTGGAAAG AGATCTTGCA 
GCTTTGGAGG ATAAGGTTAA GGCCCTCTGT GCAGAAGCTG ACCGTTTGCA GCAGTCTCAC 
CCTATAAATG CTTCTCAAAT TCAAGTGAAA CGGGAGGAAC TGATTGCCAA CTGGGAGCAG 
ATCCGAACTC TGGCAGCAGA GAGGCATGCT CGCCTTAATG ACTCCTACAG GTTGCAGCGC 
TTTCTTGCAG ATTTCCGGGA CCTCACTAGC TGGGTAACTG AGATGAAGGC TCTGATAAAT 
GCTGATGAGC TTGCCAATGA TGTGGCTGGA GCAGAAGCCC TTCTAGATAG ACATCAGGAA 
CATAAGGGAG AAATTGATGC TCATGAGGAT AGCTTCAGAT CTGCTGATGA GTCTGGGCAG 
GCTTTGCTTG CAGCTGGGCA CTATGCTTCT GATGAAGTTA AAGAAAAGCT GACTATCCTC 
TCAGATGAAA GATCAGCCTT GCTGGAACTA TGGGAGCTTC GCAGGCAACA GTATGAGCAG 
TGCATGGATC TGCAGCTTTT CTACAGAGAT ACTGAACAAG TTGACAACTG GATGAGCAAA 
CAAGAAGCTT TTCTGCTGAA CGAAGACCTT GGTGATTCTC TGGATAGCGT GGAGGCTCTT 
CTAAAGAAGC ATGAAGATTT TGAGAAATCC CTAAGTGCTC AAGAGGAGAA GATCACAGCA 
CTGGATGAGT TTGCTACTAA ACTGATTCAA AATAACCATT ATGCCATGGA TGACGTTGCT 
ACGCGCAGAG ATGCTCTTCT GAGTCGCCGA AATGCTCTTC ATGAAAGAGC CATGAAGCGC 
CGTGCCCAAC TGGCAGACTC TTTCCATCTC CAGCAGTTTT TCAGGGACTC TGATGAACTG 
AAGAGTTGGG TTAATGAAAA AATGAAAACT GCTACCGATG AGGCTTACAA GGATCCATCC 
AACTTGCAAG GTAAAGTTCA GAAGCATCAG GCTTTTGAAG CAGAGCTTTC TGCTAATCAG 
AGTCGTATTG ATGCACTGGA GAAAGCTGGC CAGAAGTTGA TTGATGTCAA TCACTATGCA 
TCTGATGAGG TGGCAGCTCG CATGAATGAA GTCATCAGCT TGTGGAAGAA ACTTCTGGAA 
GCCACCGAGC TCAAAGGTAT AAAGCTGCGT GAAGCCAATC AACAGCAGCA ATTTAATCGC 
AATGTAGAAG ACATTGAGTT GTGGCTGTAT GAAGTGGAGG GTCACTTGGC TTCTGATGAT 
TATGGAAAAG ATCTTACCAG CGTTCAGAAT CTTCAGAAGA AACATGCCCT GCTAGAGGCA 
GATGTTGCTG CCCATCAGGA TCGGATAGAT GGCATTACCA TCCAGGCACG CCAGTTCCAA 
GATGCTGGGC ACTTTGATGC TGACAATATC AAGAAGAAAC AAGAAGCTTT AGTAGCTCGT 
TATGAAGCTC TGAAGGATCC TATGGTGGCT CGCAAGCAGA AACTTGCAGA TTCTCTTCGC 
CTGCAGCAGC TTTTCCGTGA CATCGAGGAT GAAGAGACCT GGATCAGGGA AAAGGAACCT 
ATTGCAGCCT CAACAAACCG AGGCAAGGAC TTAATTGGTG TCCAGAATCT GCTAAAGAAG 
CACCAGGCTT TGCAGGCAGA AATTGCAGGC CATGAGCCTC GCATTAAAGC AGTCACACAA 
AAAGGAAATG CGATGGTGGA AGAGGGACAT TTTGCTGCTG AAGATGTGAA GATCAAATTG 
AATGAACTAA ACCAAAAATG GGACTCTCTG AAAGCCAAGG CATCTCAACG GCGACAGGAT 
CTAGAGGATT CTCTGCAAGC TCAGCAGTAT TTTGCTGATG CTAATGAGGC AGAATCATGG 
ATGAGGGAAA AGGAGCCCAT TGTAGGCAGC ACGGATTATG GAAAGGATGA AGACTCTGCT 
GAGGCTCTCC TGAAGAAGCA TGAAGCTTTG ATGTCTGATC TTTCTGCTTA CGGCAGTAGC 
ATACAGGCAT TAAGGGAACA GGCCCAGTCC TGCAGGCAAC AAGTTGCTCC CACTGATGAT 
GAAACTGGAA AAGAGCTTGT GCTAGCACTC TATGATTACC AAGAGAAGAG TCCTCGGGAG 
GTGACTATGA AGAAAGGAGA TATTCTAACC CTGCTCAACA GCACCAACAA GGACTGGTGG 
AAGGTTGAAG TTAACGATCG TCAGGGCTTT GTACCAGCTG CCTATGTGAA AAAACTAGAT 
CCTGCCCAGT CTGCATCCCG AGAGAATCTT CTAGAAGAGC AAGGCAGCAT AGCATTGCGA 
CAGGAGCAAA TTGACAACCA GACTCTCATT ACTAAGGAAG TCGGCAGTGT ATCTCTGCGT 
ATGAAACAGG TCGAAGAACT GTATCATTCC CTTCTTGAGC TGGGAGAAAA ACGTAAAGGC 
ATGCTAGAAA AAAGCTGCAA GAAGTTTATG CTTTTCCGTG AGGCTAATGA GCTTCAACAG 
TGGATCAATG AGAAGGAAGC TGCGCTCACT AATGAGGAAG TGGGTGCTGA TTTGGAGCAG 
GTGGAGGTGC TACAGAAGAA GTTTGATGAT TTTCAGAAGG ATTTAAAAGC TAATGAGTCA 
CGACTGAAGG ACATAAACAA GGTTGCAAAT GACCTGGAGT CTGAAGGGCT GATGGCAGAG 
GAAGTGCAAG CAGTAGAGCA CCAGGAAGTC TATGGAATGA TGCCCAGAGA TGAAACTGAT 
TCTAAGACAG CCTCTCCTTG GAAGTCTGCA CGTATGATGG TACACACAGT GGCAACGTTT 
AACTCAATCA AGGAACTGAA TGAACGCTGG AGATCCCTGC AGCAGTTGGC AGAGGAGCGA 
AGCCAGCTGT TGGGCAGCGC TCATGAAGTC CAGAGGTTCC ACAGAGATGC TGATGAAACA 
AAAGAATGGA TAGAAGAGAA GAATCAAGCA TTAAATACCG ACAACTATGG ACATGACTTG 
GCCAGTGTGC AGGCTCTGCA GCGCAAGCAT GAAGGCTTTG AGAGAGACTT GGCAGCTCTT 
GGAGACAAGG TGAATTCTCT TGGTGAAACT GCCCAGCGTC TGATCCAGTC ACATCCAGAA 
TCAGCTGAAG ATCTTCAAGA AAAATGTACT GAGTTGAACC AAGCCTGGAG TAGTCTGGGA 
AAACGTGCTG ACCAACGCAA AGAGAAGCTT GGAGATTCTC ATGACCTGCA GCGTTTCCTC 
AGTGATTTTA GAGACCTTAT GTCTTGGATC AATGGAATCC GGGGTCTGGT CTCCTCAGAT 
GAACTTGCAA AAGATGTGAC TGGAGCTGAA GCTCTGTTGG AAAGGCATCA GGAGCACCGT 
ACTGAAATAG ATGCACGGGC TGGCACTTTT CAGGCATTTG AACAGTTTGG ACAGCAGCTT 
CTGGCTCGTG GACACTATGC CAGCCCAGAG ATTAAGGAGA AACTGGATAT TCTAGATCAA 
GAACGGACAG ACCTAGAGAA GGCCTGGGTC CAGCGCAGAA TGATGCTAGA CCAGTGCTTA 
GAACTACAGC TGTTTCATCG GGATTGTGAA CAAGCTGAAA ACTGGATGGC TGCCCGAGAG 
GCGTTCCTAA ATACAGAAGA CAAAGGAGAC TCCTTAGACA GCGTGGAGGC ACTCATCAAG 
AAGCATGAAG ATTTCGATAA AGCAATCAAT GTCCAGGAAG AGAAAATTGC TGTCTTGCAG 
TCTTTTGCTG ACCAACTGAT TGCTGCTGAT CATTATGCAA AAGGAGTCAT TGCTAACAGA 
CGCAATGAGG TTCTGGACAG GTGGCGTCGT CTGAAGGCTC AGATGATTGA GAAGAGATCT 
AAGCTGGGAG AATCTCAGAC CCTCCAACAG TTCAGTCGTG ATGTTGATGA AATAGAAGCT 
TGGATCAGTG AAAAGCTTCA AACTGCAAGT GATGAGTCAT ATAAGGATCC CACAAACATC 
CAGCTTTCCA AACTGCTGAG CAAGCACCAG AAGCACCAAG CCTTTGAAGC TGAGCTCCAC 
GCCAACGCAG ATCGGATTCG TGGAGTCATT GAAATGGGGA ACTCTCTTAT TGAAAGAGGA 
GCGTGTGCTG GCAGCGAGGA TGCTGTGAAG GCTCGGCTGG CTGCCCTGGC TGACCAATGG 
GAGTTCCTGG TCCAGAAGTC ATCAGAGAAG AGTCAGAAAC TGAAAGAAGC AAATAAACAG 
CAGAATTTCA ATACCGGCAT CAAGGACTTT GATTTCTGGC TTTCAGAGGT GGAAGCTTTG 
TTGGCATCTG AAGACTATGG GAAGGACTTG GCATCAGTGA ACAACCTTCT GAAGAAGCAC 
CAATTACTGG AAGCTGATAT ATCTGCTCAT GAGGATCGGC TGAAGGACCT GAACAGCCAG 
GCTGACAGTT TGATGACCAG CAGTGCTTTC GATACCTCCC AAGTAAAGGA TAAACGTGAA 
ACTATAAATG GGCGCTTCCA GAGAATCAAG AGCATGGCAG CTGCCCGCCG TGCGAAGCTC 
AACGAGTCGC ACCGCTTGCA TCAGTTCTTC CGTGACATGG ATGATGAGGA GTCCTGGATC 
AAAGAGAAGA AACTGTTGGT GAGCTCAGAG GACTATGGCA GAGACCTGAC TGGTGTGCAG 
AACCTGAGGA AGAAACATAA GCGCTTGGAA GCAGAATTAG CTGCCCATGA ACCTGCTATC 
CAGGGTGTTC TAGACACTGG CAAGAAGCTT TCAGATGATA ACACAATTGG GAAGGAGGAG 
ATACAGCAAA GACTGGCTCA GTTTGTGGAC CACTGGAAAG AGTTAAAACA GCTGGCAGCT 
GCTCGGGGGC AGCGTCTGGA GGAGTCCCTG GAGTACCAAC AGTTTGTAGC AAATGTTGAG 
GAAGAGGAGG CATGGATCAA CGAGAAAATG ACATTGGTAG CCAGTGAGGA TTATGGAGAC 
ACACTTGCTG CTATCCAGGG CTTGCTGAAG AAGCATGAAG CATTCGAGAC TGACTTTACT 
GTCCACAAAG ACAGAGTGAA TGATGTTTGC GCTAATGGAG AGGATCTCAT TAAAAAGAAT 
AATCACCATG TGGAGAACAT TACTGCTAAG ATGAAGGGCC TCAAGGGCAA GGTATCAGAT 
CTGGAGAAAG CCGCAGCTCA GAGGAAAGCC AAACTGGATG AGAACTCTGC CTTCCTCCAG 
TTCAACTGGA AAGCAGATGT GGTGGAGTCA TGGATAGGTG AGAAGGAAAA CAGTCTGAAG 
ACAGATGATT ATGGCCGTGA CCTCTCCTCT GTGCAAACAC TACTCACCAA ACAGGAAACC 
TTTGATGCTG GACTTCAGGC TTTCCAGCAG GAGGGAATTG CAAACATCAC TGCTCTGAAA 
GACCAGCTAC TGGCAGCCAA ACATATCCAA TCAAAGGCCA TTGAGGTTCG TCACGCTTCC 
TTGATGAAAC GCTGGAATCA GCTGCTTGCT AATTCTGCAG CCAGGAAAAA GAAACTCTTG 
GAGGCTCAGG AGCACTTCAG AAAGGTTGAA GATCTCTTCT TGACTTTTGC CAAGAAGGCC 
TCTGCCTTCA ACAGTTGGTT TGAGAATGCT GAGGAGGACC TGACGGATCC CGTGCGCTGC 
AATTCCCTGG AAGAAATCAA AGCACTGCGA GAAGCCCACG ATGCGTTCCG TTCCTCACTT 
AGCTCTGCCC AAGCTGACTT CAACCAGCTG GCAGAGCTTG ATCGGCAGAT CAAGAGCTTC 
CGTGTAGCCT CCAACCCGTA CACTTGGTTT ACTATGGAGG CTCTTGAAGA AACTTGGAGG 
AATCTGCAGA AAATTATCAA GGAGCGTGAA TTGGAGTTGC AGAAGGAGCA GCGGAGGCAG 
GAAGAAAATG ACAAGTTGCG CCAGGAGTTT GCTCAGCATG CTAATGCCTT CCACCAGTGG 
ATTCAGGAGA CAAGGACTTA CCTGCTAGAT GGGTCCTGCA TGGTGGAGGA GTCGGGAACA 
CTGGAATCCC AGCTGGAAGC TACTAAACGC AAGCACCAGG AGATCCGGGC TATGAGGAGC 
CAGCTGAAGA AGATTGAGGA CCTTGGAGCA GCCATGGAAG AGGCACTTAT CTTGGACAAC 
AAATACACAG AGCACAGCAC CGTGGGGCTG GCGCAGCAGT GGGACCAGCT TGACCAGCTG 
GGAATGAGAA TGCAGCACAA TCTGGAACAG CAGATTCAAG CTCGAAACAC AACTGGAGTC 
ACAGAGGAGG CCCTGAAGGA GTTCAGCATG ATGTTCAAGC ACTTTGACAA GGACAAATCT 
GGACGTCTTA ATCACCAGGA GTTTAAGTCT TGCTTGCGCT CCCTTGGCTA TGACCTGCCC 
ATGGTTGAGG AAGGAGAGCC TGACCCCGAA TTTGAGTCTA TTCTTGACAC TGTTGATCCC 
AACAGGGATG GCCACGTCTC GCTGCAGGAG TACATGGCGT TCATGATCAG CAGGGAAACA 
GAGAACGTGA AATCCAGCGA GGAGATTGAG AGTGCTTTCC GTGCCCTCAG CTCGGAGGGG 
AAGCCTTACG TGACCAAGGA GGAGCTCTAC CAGAACCTGA CCCGGGAGCA GGCCGACTAC 
TGCATTTCTC ACATGAAGCC CTACATGGAT GGCAAGGGCA GAGAACTTCC TTCTGCCTAC 
GACTACATAG AATTTACACG TTCACTCTTT GTGAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015134772.1
CDS	13..7551
Translation	MLSSFRKVKVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSSLRRQKLEDSYRFQFFQRDADELEKWIQEKLQIASDENYKDPSNLQGKLQKHQAFEAEVQANSGAIVKLDETGNQMINEGHFASETIRSFTPYRTAQRPIVTATQVGSHIRTRLQELHRLWELLLEKMREKGVKLLQAQKLVQFLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDLAAHEERVNEVNQFAGKLIQEQHPEEELIKSKQDEVNASWQRLKGLAQQRQGKLFGAAEVQRFNRDVDETISWIKEKGQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPINASQIQVKREELIANWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFRSADESGQALLAAGHYASDEVKEKLTILSDERSALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMDDVATRRDALLSRRNALHERAMKRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYASDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTSVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDADNIKKKQEALVARYEALKDPMVARKQKLADSLRLQQLFRDIEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKIKLNELNQKWDSLKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPTDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTLITKEVGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTNEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVANDLESEGLMAEEVQAVEHQEVYGMMPRDETDSKTASPWKSARMMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAQRLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKEKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLARGHYASPEIKEKLDILDQERTDLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAVLQSFADQLIAADHYAKGVIANRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIEMGNSLIERGACAGSEDAVKARLAALADQWEFLVQKSSEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRETINGRFQRIKSMAAARRAKLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVDHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCANGEDLIKKNNHHVENITAKMKGLKGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHIQSKAIEVRHASLMKRWNQLLANSAARKKKLLEAQEHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFESILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCISHMKPYMDGKGRELPSAYDYIEFTRSLFVN

Target ORF information:

RefSeq Version	XM_015279286.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279286.1

ORF Insert Sequence:

ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG 
TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC 
TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT 
GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT 
GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG 
ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAT CTTTCACGCC TTACAGGACT 
GCACAGCGCC CAATAGTAAC AGCCACCCAA GTTGGGTCCC ACATACGAAC TCGTCTACAG 
GAGCTGCACC GACTATGGGA GTTACTGCTG GAAAAAATGA GAGAGAAGGG AGTGAAGCTG 
TTGCAAGCAC AGAAGCTGGT GCAATTCTTA CGGGAATGTG AAGATGTCAT GGACTGGATC 
AATGACAAGG AAGCAATAGT GACCTCAGAA GAGCTTGGAC AGGACTTAGA GCATGTTGAG 
GTTTTGCAGA AGAAATTTGA AGAGTTCCAA ACAGATCTGG CAGCTCATGA GGAGAGAGTG 
AATGAAGTGA ACCAGTTCGC TGGCAAACTT ATCCAGGAAC AGCACCCTGA GGAGGAACTT 
ATAAAGTCCA AACAGGATGA AGTAAATGCA AGCTGGCAGC GTCTTAAGGG GCTTGCCCAA 
CAGAGGCAAG GAAAACTCTT TGGGGCAGCT GAAGTTCAGC GCTTCAACAG GGATGTGGAT 
GAAACTATCA GCTGGATTAA GGAGAAAGGG CAGTTGATGG CCTCAGATGA CTTTGGCAGA 
GACTTGGCCA GCGTGCAAGC TTTACTACGT AAGCATGAAG GCCTGGAAAG AGATCTTGCA 
GCTTTGGAGG ATAAGGTTAA GGCCCTCTGT GCAGAAGCTG ACCGTTTGCA GCAGTCTCAC 
CCTATAAATG CTTCTCAAAT TCAAGTGAAA CGGGAGGAAC TGATTGCCAA CTGGGAGCAG 
ATCCGAACTC TGGCAGCAGA GAGGCATGCT CGCCTTAATG ACTCCTACAG GTTGCAGCGC 
TTTCTTGCAG ATTTCCGGGA CCTCACTAGC TGGGTAACTG AGATGAAGGC TCTGATAAAT 
GCTGATGAGC TTGCCAATGA TGTGGCTGGA GCAGAAGCCC TTCTAGATAG ACATCAGGAA 
CATAAGGGAG AAATTGATGC TCATGAGGAT AGCTTCAGAT CTGCTGATGA GTCTGGGCAG 
GCTTTGCTTG CAGCTGGGCA CTATGCTTCT GATGAAGTTA AAGAAAAGCT GACTATCCTC 
TCAGATGAAA GATCAGCCTT GCTGGAACTA TGGGAGCTTC GCAGGCAACA GTATGAGCAG 
TGCATGGATC TGCAGCTTTT CTACAGAGAT ACTGAACAAG TTGACAACTG GATGAGCAAA 
CAAGAAGCTT TTCTGCTGAA CGAAGACCTT GGTGATTCTC TGGATAGCGT GGAGGCTCTT 
CTAAAGAAGC ATGAAGATTT TGAGAAATCC CTAAGTGCTC AAGAGGAGAA GATCACAGCA 
CTGGATGAGT TTGCTACTAA ACTGATTCAA AATAACCATT ATGCCATGGA TGACGTTGCT 
ACGCGCAGAG ATGCTCTTCT GAGTCGCCGA AATGCTCTTC ATGAAAGAGC CATGAAGCGC 
CGTGCCCAAC TGGCAGACTC TTTCCATCTC CAGCAGTTTT TCAGGGACTC TGATGAACTG 
AAGAGTTGGG TTAATGAAAA AATGAAAACT GCTACCGATG AGGCTTACAA GGATCCATCC 
AACTTGCAAG GTAAAGTTCA GAAGCATCAG GCTTTTGAAG CAGAGCTTTC TGCTAATCAG 
AGTCGTATTG ATGCACTGGA GAAAGCTGGC CAGAAGTTGA TTGATGTCAA TCACTATGCA 
TCTGATGAGG TGGCAGCTCG CATGAATGAA GTCATCAGCT TGTGGAAGAA ACTTCTGGAA 
GCCACCGAGC TCAAAGGTAT AAAGCTGCGT GAAGCCAATC AACAGCAGCA ATTTAATCGC 
AATGTAGAAG ACATTGAGTT GTGGCTGTAT GAAGTGGAGG GTCACTTGGC TTCTGATGAT 
TATGGAAAAG ATCTTACCAG CGTTCAGAAT CTTCAGAAGA AACATGCCCT GCTAGAGGCA 
GATGTTGCTG CCCATCAGGA TCGGATAGAT GGCATTACCA TCCAGGCACG CCAGTTCCAA 
GATGCTGGGC ACTTTGATGC TGACAATATC AAGAAGAAAC AAGAAGCTTT AGTAGCTCGT 
TATGAAGCTC TGAAGGATCC TATGGTGGCT CGCAAGCAGA AACTTGCAGA TTCTCTTCGC 
CTGCAGCAGC TTTTCCGTGA CATCGAGGAT GAAGAGACCT GGATCAGGGA AAAGGAACCT 
ATTGCAGCCT CAACAAACCG AGGCAAGGAC TTAATTGGTG TCCAGAATCT GCTAAAGAAG 
CACCAGGCTT TGCAGGCAGA AATTGCAGGC CATGAGCCTC GCATTAAAGC AGTCACACAA 
AAAGGAAATG CGATGGTGGA AGAGGGACAT TTTGCTGCTG AAGATGTGAA GATCAAATTG 
AATGAACTAA ACCAAAAATG GGACTCTCTG AAAGCCAAGG CATCTCAACG GCGACAGGAT 
CTAGAGGATT CTCTGCAAGC TCAGCAGTAT TTTGCTGATG CTAATGAGGC AGAATCATGG 
ATGAGGGAAA AGGAGCCCAT TGTAGGCAGC ACGGATTATG GAAAGGATGA AGACTCTGCT 
GAGGCTCTCC TGAAGAAGCA TGAAGCTTTG ATGTCTGATC TTTCTGCTTA CGGCAGTAGC 
ATACAGGCAT TAAGGGAACA GGCCCAGTCC TGCAGGCAAC AAGTTGCTCC CACTGATGAT 
GAAACTGGAA AAGAGCTTGT GCTAGCACTC TATGATTACC AAGAGAAGAG TCCTCGGGAG 
GTGACTATGA AGAAAGGAGA TATTCTAACC CTGCTCAACA GCACCAACAA GGACTGGTGG 
AAGGTTGAAG TTAACGATCG TCAGGGCTTT GTACCAGCTG CCTATGTGAA AAAACTAGAT 
CCTGCCCAGT CTGCATCCCG AGAGAATCTT CTAGAAGAGC AAGGCAGCAT AGCATTGCGA 
CAGGAGCAAA TTGACAACCA GACTCTCATT ACTAAGGAAG TCGGCAGTGT ATCTCTGCGT 
ATGAAACAGG TCGAAGAACT GTATCATTCC CTTCTTGAGC TGGGAGAAAA ACGTAAAGGC 
ATGCTAGAAA AAAGCTGCAA GAAGTTTATG CTTTTCCGTG AGGCTAATGA GCTTCAACAG 
TGGATCAATG AGAAGGAAGC TGCGCTCACT AATGAGGAAG TGGGTGCTGA TTTGGAGCAG 
GTGGAGGTGC TACAGAAGAA GTTTGATGAT TTTCAGAAGG ATTTAAAAGC TAATGAGTCA 
CGACTGAAGG ACATAAACAA GGTTGCAAAT GACCTGGAGT CTGAAGGGCT GATGGCAGAG 
GAAGTGCAAG CAGTAGAGCA CCAGGAAGTC TATGGAATGA TGCCCAGAGA TGAAACTGAT 
TCTAAGACAG CCTCTCCTTG GAAGTCTGCA CGTATGATGG TACACACAGT GGCAACGTTT 
AACTCAATCA AGGAACTGAA TGAACGCTGG AGATCCCTGC AGCAGTTGGC AGAGGAGCGA 
AGCCAGCTGT TGGGCAGCGC TCATGAAGTC CAGAGGTTCC ACAGAGATGC TGATGAAACA 
AAAGAATGGA TAGAAGAGAA GAATCAAGCA TTAAATACCG ACAACTATGG ACATGACTTG 
GCCAGTGTGC AGGCTCTGCA GCGCAAGCAT GAAGGCTTTG AGAGAGACTT GGCAGCTCTT 
GGAGACAAGG TGAATTCTCT TGGTGAAACT GCCCAGCGTC TGATCCAGTC ACATCCAGAA 
TCAGCTGAAG ATCTTCAAGA AAAATGTACT GAGTTGAACC AAGCCTGGAG TAGTCTGGGA 
AAACGTGCTG ACCAACGCAA AGAGAAGCTT GGAGATTCTC ATGACCTGCA GCGTTTCCTC 
AGTGATTTTA GAGACCTTAT GTCTTGGATC AATGGAATCC GGGGTCTGGT CTCCTCAGAT 
GAACTTGCAA AAGATGTGAC TGGAGCTGAA GCTCTGTTGG AAAGGCATCA GGAGCACCGT 
ACTGAAATAG ATGCACGGGC TGGCACTTTT CAGGCATTTG AACAGTTTGG ACAGCAGCTT 
CTGGCTCGTG GACACTATGC CAGCCCAGAG ATTAAGGAGA AACTGGATAT TCTAGATCAA 
GAACGGACAG ACCTAGAGAA GGCCTGGGTC CAGCGCAGAA TGATGCTAGA CCAGTGCTTA 
GAACTACAGC TGTTTCATCG GGATTGTGAA CAAGCTGAAA ACTGGATGGC TGCCCGAGAG 
GCGTTCCTAA ATACAGAAGA CAAAGGAGAC TCCTTAGACA GCGTGGAGGC ACTCATCAAG 
AAGCATGAAG ATTTCGATAA AGCAATCAAT GTCCAGGAAG AGAAAATTGC TGTCTTGCAG 
TCTTTTGCTG ACCAACTGAT TGCTGCTGAT CATTATGCAA AAGGAGTCAT TGCTAACAGA 
CGCAATGAGG TTCTGGACAG GTGGCGTCGT CTGAAGGCTC AGATGATTGA GAAGAGATCT 
AAGCTGGGAG AATCTCAGAC CCTCCAACAG TTCAGTCGTG ATGTTGATGA AATAGAAGCT 
TGGATCAGTG AAAAGCTTCA AACTGCAAGT GATGAGTCAT ATAAGGATCC CACAAACATC 
CAGCTTTCCA AACTGCTGAG CAAGCACCAG AAGCACCAAG CCTTTGAAGC TGAGCTCCAC 
GCCAACGCAG ATCGGATTCG TGGAGTCATT GAAATGGGGA ACTCTCTTAT TGAAAGAGGA 
GCGTGTGCTG GCAGCGAGGA TGCTGTGAAG GCTCGGCTGG CTGCCCTGGC TGACCAATGG 
GAGTTCCTGG TCCAGAAGTC ATCAGAGAAG AGTCAGAAAC TGAAAGAAGC AAATAAACAG 
CAGAATTTCA ATACCGGCAT CAAGGACTTT GATTTCTGGC TTTCAGAGGT GGAAGCTTTG 
TTGGCATCTG AAGACTATGG GAAGGACTTG GCATCAGTGA ACAACCTTCT GAAGAAGCAC 
CAATTACTGG AAGCTGATAT ATCTGCTCAT GAGGATCGGC TGAAGGACCT GAACAGCCAG 
GCTGACAGTT TGATGACCAG CAGTGCTTTC GATACCTCCC AAGTAAAGGA TAAACGTGAA 
ACTATAAATG GGCGCTTCCA GAGAATCAAG AGCATGGCAG CTGCCCGCCG TGCGAAGCTC 
AACGAGTCGC ACCGCTTGCA TCAGTTCTTC CGTGACATGG ATGATGAGGA GTCCTGGATC 
AAAGAGAAGA AACTGTTGGT GAGCTCAGAG GACTATGGCA GAGACCTGAC TGGTGTGCAG 
AACCTGAGGA AGAAACATAA GCGCTTGGAA GCAGAATTAG CTGCCCATGA ACCTGCTATC 
CAGGGTGTTC TAGACACTGG CAAGAAGCTT TCAGATGATA ACACAATTGG GAAGGAGGAG 
ATACAGCAAA GACTGGCTCA GTTTGTGGAC CACTGGAAAG AGTTAAAACA GCTGGCAGCT 
GCTCGGGGGC AGCGTCTGGA GGAGTCCCTG GAGTACCAAC AGTTTGTAGC AAATGTTGAG 
GAAGAGGAGG CATGGATCAA CGAGAAAATG ACATTGGTAG CCAGTGAGGA TTATGGAGAC 
ACACTTGCTG CTATCCAGGG CTTGCTGAAG AAGCATGAAG CATTCGAGAC TGACTTTACT 
GTCCACAAAG ACAGAGTGAA TGATGTTTGC GCTAATGGAG AGGATCTCAT TAAAAAGAAT 
AATCACCATG TGGAGAACAT TACTGCTAAG ATGAAGGGCC TCAAGGGCAA GGTATCAGAT 
CTGGAGAAAG CCGCAGCTCA GAGGAAAGCC AAACTGGATG AGAACTCTGC CTTCCTCCAG 
TTCAACTGGA AAGCAGATGT GGTGGAGTCA TGGATAGGTG AGAAGGAAAA CAGTCTGAAG 
ACAGATGATT ATGGCCGTGA CCTCTCCTCT GTGCAAACAC TACTCACCAA ACAGGAAACC 
TTTGATGCTG GACTTCAGGC TTTCCAGCAG GAGGGAATTG CAAACATCAC TGCTCTGAAA 
GACCAGCTAC TGGCAGCCAA ACATATCCAA TCAAAGGCCA TTGAGGTTCG TCACGCTTCC 
TTGATGAAAC GCTGGAATCA GCTGCTTGCT AATTCTGCAG CCAGGAAAAA GAAACTCTTG 
GAGGCTCAGG AGCACTTCAG AAAGGTTGAA GATCTCTTCT TGACTTTTGC CAAGAAGGCC 
TCTGCCTTCA ACAGTTGGTT TGAGAATGCT GAGGAGGACC TGACGGATCC CGTGCGCTGC 
AATTCCCTGG AAGAAATCAA AGCACTGCGA GAAGCCCACG ATGCGTTCCG TTCCTCACTT 
AGCTCTGCCC AAGCTGACTT CAACCAGCTG GCAGAGCTTG ATCGGCAGAT CAAGAGCTTC 
CGTGTAGCCT CCAACCCGTA CACTTGGTTT ACTATGGAGG CTCTTGAAGA AACTTGGAGG 
AATCTGCAGA AAATTATCAA GGAGCGTGAA TTGGAGTTGC AGAAGGAGCA GCGGAGGCAG 
GAAGAAAATG ACAAGTTGCG CCAGGAGTTT GCTCAGCATG CTAATGCCTT CCACCAGTGG 
ATTCAGGAGA CAAGGACTTA CCTGCTAGAT GGGTCCTGCA TGGTGGAGGA GTCGGGAACA 
CTGGAATCCC AGCTGGAAGC TACTAAACGC AAGCACCAGG AGATCCGGGC TATGAGGAGC 
CAGCTGAAGA AGATTGAGGA CCTTGGAGCA GCCATGGAAG AGGCACTTAT CTTGGACAAC 
AAATACACAG AGCACAGCAC CGTGGGGCTG GCGCAGCAGT GGGACCAGCT TGACCAGCTG 
GGAATGAGAA TGCAGCACAA TCTGGAACAG CAGATTCAAG CTCGAAACAC AACTGGAGTC 
ACAGAGGAGG CCCTGAAGGA GTTCAGCATG ATGTTCAAGC ACTTTGACAA GGACAAATCT 
GGACGTCTTA ATCACCAGGA GTTTAAGTCT TGCTTGCGCT CCCTTGGCTA TGACCTGCCC 
ATGGTTGAGG AAGGAGAGCC TGACCCCGAA TTTGAGTCTA TTCTTGACAC TGTTGATCCC 
AACAGGGATG GCCACGTCTC GCTGCAGGAG TACATGGCGT TCATGATCAG CAGGGAAACA 
GAGAACGTGA AATCCAGCGA GGAGATTGAG AGTGCTTTCC GTGCCCTCAG CTCGGAGGGG 
AAGCCTTACG TGACCAAGGA GGAGCTCTAC CAGAACCTGA CCCGGGAGCA GGCCGACTAC 
TGCATTTCTC ACATGAAGCC CTACATGGAT GGCAAGGGCA GAGAACTTCC TTCTGCCTAC 
GACTACATAG AATTTACACG TTCACTCTTT GTGAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_015279286.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015134772.1 Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X5, mRNA.

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CloneID	OGa43312
Clone ID Related Accession (Same CDS sequence)	XM_015279286.1 , XM_015279286.2
Accession Version	XM_015279286.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7539bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015279286.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG 
TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC 
TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT 
GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT 
GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG 
ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAT CTTTCACGCC TTACAGGACT 
GCACAGCGCC CAATAGTAAC AGCCACCCAA GTTGGGTCCC ACATACGAAC TCGTCTACAG 
GAGCTGCACC GACTATGGGA GTTACTGCTG GAAAAAATGA GAGAGAAGGG AGTGAAGCTG 
TTGCAAGCAC AGAAGCTGGT GCAATTCTTA CGGGAATGTG AAGATGTCAT GGACTGGATC 
AATGACAAGG AAGCAATAGT GACCTCAGAA GAGCTTGGAC AGGACTTAGA GCATGTTGAG 
GTTTTGCAGA AGAAATTTGA AGAGTTCCAA ACAGATCTGG CAGCTCATGA GGAGAGAGTG 
AATGAAGTGA ACCAGTTCGC TGGCAAACTT ATCCAGGAAC AGCACCCTGA GGAGGAACTT 
ATAAAGTCCA AACAGGATGA AGTAAATGCA AGCTGGCAGC GTCTTAAGGG GCTTGCCCAA 
CAGAGGCAAG GAAAACTCTT TGGGGCAGCT GAAGTTCAGC GCTTCAACAG GGATGTGGAT 
GAAACTATCA GCTGGATTAA GGAGAAAGGG CAGTTGATGG CCTCAGATGA CTTTGGCAGA 
GACTTGGCCA GCGTGCAAGC TTTACTACGT AAGCATGAAG GCCTGGAAAG AGATCTTGCA 
GCTTTGGAGG ATAAGGTTAA GGCCCTCTGT GCAGAAGCTG ACCGTTTGCA GCAGTCTCAC 
CCTATAAATG CTTCTCAAAT TCAAGTGAAA CGGGAGGAAC TGATTGCCAA CTGGGAGCAG 
ATCCGAACTC TGGCAGCAGA GAGGCATGCT CGCCTTAATG ACTCCTACAG GTTGCAGCGC 
TTTCTTGCAG ATTTCCGGGA CCTCACTAGC TGGGTAACTG AGATGAAGGC TCTGATAAAT 
GCTGATGAGC TTGCCAATGA TGTGGCTGGA GCAGAAGCCC TTCTAGATAG ACATCAGGAA 
CATAAGGGAG AAATTGATGC TCATGAGGAT AGCTTCAGAT CTGCTGATGA GTCTGGGCAG 
GCTTTGCTTG CAGCTGGGCA CTATGCTTCT GATGAAGTTA AAGAAAAGCT GACTATCCTC 
TCAGATGAAA GATCAGCCTT GCTGGAACTA TGGGAGCTTC GCAGGCAACA GTATGAGCAG 
TGCATGGATC TGCAGCTTTT CTACAGAGAT ACTGAACAAG TTGACAACTG GATGAGCAAA 
CAAGAAGCTT TTCTGCTGAA CGAAGACCTT GGTGATTCTC TGGATAGCGT GGAGGCTCTT 
CTAAAGAAGC ATGAAGATTT TGAGAAATCC CTAAGTGCTC AAGAGGAGAA GATCACAGCA 
CTGGATGAGT TTGCTACTAA ACTGATTCAA AATAACCATT ATGCCATGGA TGACGTTGCT 
ACGCGCAGAG ATGCTCTTCT GAGTCGCCGA AATGCTCTTC ATGAAAGAGC CATGAAGCGC 
CGTGCCCAAC TGGCAGACTC TTTCCATCTC CAGCAGTTTT TCAGGGACTC TGATGAACTG 
AAGAGTTGGG TTAATGAAAA AATGAAAACT GCTACCGATG AGGCTTACAA GGATCCATCC 
AACTTGCAAG GTAAAGTTCA GAAGCATCAG GCTTTTGAAG CAGAGCTTTC TGCTAATCAG 
AGTCGTATTG ATGCACTGGA GAAAGCTGGC CAGAAGTTGA TTGATGTCAA TCACTATGCA 
TCTGATGAGG TGGCAGCTCG CATGAATGAA GTCATCAGCT TGTGGAAGAA ACTTCTGGAA 
GCCACCGAGC TCAAAGGTAT AAAGCTGCGT GAAGCCAATC AACAGCAGCA ATTTAATCGC 
AATGTAGAAG ACATTGAGTT GTGGCTGTAT GAAGTGGAGG GTCACTTGGC TTCTGATGAT 
TATGGAAAAG ATCTTACCAG CGTTCAGAAT CTTCAGAAGA AACATGCCCT GCTAGAGGCA 
GATGTTGCTG CCCATCAGGA TCGGATAGAT GGCATTACCA TCCAGGCACG CCAGTTCCAA 
GATGCTGGGC ACTTTGATGC TGACAATATC AAGAAGAAAC AAGAAGCTTT AGTAGCTCGT 
TATGAAGCTC TGAAGGATCC TATGGTGGCT CGCAAGCAGA AACTTGCAGA TTCTCTTCGC 
CTGCAGCAGC TTTTCCGTGA CATCGAGGAT GAAGAGACCT GGATCAGGGA AAAGGAACCT 
ATTGCAGCCT CAACAAACCG AGGCAAGGAC TTAATTGGTG TCCAGAATCT GCTAAAGAAG 
CACCAGGCTT TGCAGGCAGA AATTGCAGGC CATGAGCCTC GCATTAAAGC AGTCACACAA 
AAAGGAAATG CGATGGTGGA AGAGGGACAT TTTGCTGCTG AAGATGTGAA GATCAAATTG 
AATGAACTAA ACCAAAAATG GGACTCTCTG AAAGCCAAGG CATCTCAACG GCGACAGGAT 
CTAGAGGATT CTCTGCAAGC TCAGCAGTAT TTTGCTGATG CTAATGAGGC AGAATCATGG 
ATGAGGGAAA AGGAGCCCAT TGTAGGCAGC ACGGATTATG GAAAGGATGA AGACTCTGCT 
GAGGCTCTCC TGAAGAAGCA TGAAGCTTTG ATGTCTGATC TTTCTGCTTA CGGCAGTAGC 
ATACAGGCAT TAAGGGAACA GGCCCAGTCC TGCAGGCAAC AAGTTGCTCC CACTGATGAT 
GAAACTGGAA AAGAGCTTGT GCTAGCACTC TATGATTACC AAGAGAAGAG TCCTCGGGAG 
GTGACTATGA AGAAAGGAGA TATTCTAACC CTGCTCAACA GCACCAACAA GGACTGGTGG 
AAGGTTGAAG TTAACGATCG TCAGGGCTTT GTACCAGCTG CCTATGTGAA AAAACTAGAT 
CCTGCCCAGT CTGCATCCCG AGAGAATCTT CTAGAAGAGC AAGGCAGCAT AGCATTGCGA 
CAGGAGCAAA TTGACAACCA GACTCTCATT ACTAAGGAAG TCGGCAGTGT ATCTCTGCGT 
ATGAAACAGG TCGAAGAACT GTATCATTCC CTTCTTGAGC TGGGAGAAAA ACGTAAAGGC 
ATGCTAGAAA AAAGCTGCAA GAAGTTTATG CTTTTCCGTG AGGCTAATGA GCTTCAACAG 
TGGATCAATG AGAAGGAAGC TGCGCTCACT AATGAGGAAG TGGGTGCTGA TTTGGAGCAG 
GTGGAGGTGC TACAGAAGAA GTTTGATGAT TTTCAGAAGG ATTTAAAAGC TAATGAGTCA 
CGACTGAAGG ACATAAACAA GGTTGCAAAT GACCTGGAGT CTGAAGGGCT GATGGCAGAG 
GAAGTGCAAG CAGTAGAGCA CCAGGAAGTC TATGGAATGA TGCCCAGAGA TGAAACTGAT 
TCTAAGACAG CCTCTCCTTG GAAGTCTGCA CGTATGATGG TACACACAGT GGCAACGTTT 
AACTCAATCA AGGAACTGAA TGAACGCTGG AGATCCCTGC AGCAGTTGGC AGAGGAGCGA 
AGCCAGCTGT TGGGCAGCGC TCATGAAGTC CAGAGGTTCC ACAGAGATGC TGATGAAACA 
AAAGAATGGA TAGAAGAGAA GAATCAAGCA TTAAATACCG ACAACTATGG ACATGACTTG 
GCCAGTGTGC AGGCTCTGCA GCGCAAGCAT GAAGGCTTTG AGAGAGACTT GGCAGCTCTT 
GGAGACAAGG TGAATTCTCT TGGTGAAACT GCCCAGCGTC TGATCCAGTC ACATCCAGAA 
TCAGCTGAAG ATCTTCAAGA AAAATGTACT GAGTTGAACC AAGCCTGGAG TAGTCTGGGA 
AAACGTGCTG ACCAACGCAA AGAGAAGCTT GGAGATTCTC ATGACCTGCA GCGTTTCCTC 
AGTGATTTTA GAGACCTTAT GTCTTGGATC AATGGAATCC GGGGTCTGGT CTCCTCAGAT 
GAACTTGCAA AAGATGTGAC TGGAGCTGAA GCTCTGTTGG AAAGGCATCA GGAGCACCGT 
ACTGAAATAG ATGCACGGGC TGGCACTTTT CAGGCATTTG AACAGTTTGG ACAGCAGCTT 
CTGGCTCGTG GACACTATGC CAGCCCAGAG ATTAAGGAGA AACTGGATAT TCTAGATCAA 
GAACGGACAG ACCTAGAGAA GGCCTGGGTC CAGCGCAGAA TGATGCTAGA CCAGTGCTTA 
GAACTACAGC TGTTTCATCG GGATTGTGAA CAAGCTGAAA ACTGGATGGC TGCCCGAGAG 
GCGTTCCTAA ATACAGAAGA CAAAGGAGAC TCCTTAGACA GCGTGGAGGC ACTCATCAAG 
AAGCATGAAG ATTTCGATAA AGCAATCAAT GTCCAGGAAG AGAAAATTGC TGTCTTGCAG 
TCTTTTGCTG ACCAACTGAT TGCTGCTGAT CATTATGCAA AAGGAGTCAT TGCTAACAGA 
CGCAATGAGG TTCTGGACAG GTGGCGTCGT CTGAAGGCTC AGATGATTGA GAAGAGATCT 
AAGCTGGGAG AATCTCAGAC CCTCCAACAG TTCAGTCGTG ATGTTGATGA AATAGAAGCT 
TGGATCAGTG AAAAGCTTCA AACTGCAAGT GATGAGTCAT ATAAGGATCC CACAAACATC 
CAGCTTTCCA AACTGCTGAG CAAGCACCAG AAGCACCAAG CCTTTGAAGC TGAGCTCCAC 
GCCAACGCAG ATCGGATTCG TGGAGTCATT GAAATGGGGA ACTCTCTTAT TGAAAGAGGA 
GCGTGTGCTG GCAGCGAGGA TGCTGTGAAG GCTCGGCTGG CTGCCCTGGC TGACCAATGG 
GAGTTCCTGG TCCAGAAGTC ATCAGAGAAG AGTCAGAAAC TGAAAGAAGC AAATAAACAG 
CAGAATTTCA ATACCGGCAT CAAGGACTTT GATTTCTGGC TTTCAGAGGT GGAAGCTTTG 
TTGGCATCTG AAGACTATGG GAAGGACTTG GCATCAGTGA ACAACCTTCT GAAGAAGCAC 
CAATTACTGG AAGCTGATAT ATCTGCTCAT GAGGATCGGC TGAAGGACCT GAACAGCCAG 
GCTGACAGTT TGATGACCAG CAGTGCTTTC GATACCTCCC AAGTAAAGGA TAAACGTGAA 
ACTATAAATG GGCGCTTCCA GAGAATCAAG AGCATGGCAG CTGCCCGCCG TGCGAAGCTC 
AACGAGTCGC ACCGCTTGCA TCAGTTCTTC CGTGACATGG ATGATGAGGA GTCCTGGATC 
AAAGAGAAGA AACTGTTGGT GAGCTCAGAG GACTATGGCA GAGACCTGAC TGGTGTGCAG 
AACCTGAGGA AGAAACATAA GCGCTTGGAA GCAGAATTAG CTGCCCATGA ACCTGCTATC 
CAGGGTGTTC TAGACACTGG CAAGAAGCTT TCAGATGATA ACACAATTGG GAAGGAGGAG 
ATACAGCAAA GACTGGCTCA GTTTGTGGAC CACTGGAAAG AGTTAAAACA GCTGGCAGCT 
GCTCGGGGGC AGCGTCTGGA GGAGTCCCTG GAGTACCAAC AGTTTGTAGC AAATGTTGAG 
GAAGAGGAGG CATGGATCAA CGAGAAAATG ACATTGGTAG CCAGTGAGGA TTATGGAGAC 
ACACTTGCTG CTATCCAGGG CTTGCTGAAG AAGCATGAAG CATTCGAGAC TGACTTTACT 
GTCCACAAAG ACAGAGTGAA TGATGTTTGC GCTAATGGAG AGGATCTCAT TAAAAAGAAT 
AATCACCATG TGGAGAACAT TACTGCTAAG ATGAAGGGCC TCAAGGGCAA GGTATCAGAT 
CTGGAGAAAG CCGCAGCTCA GAGGAAAGCC AAACTGGATG AGAACTCTGC CTTCCTCCAG 
TTCAACTGGA AAGCAGATGT GGTGGAGTCA TGGATAGGTG AGAAGGAAAA CAGTCTGAAG 
ACAGATGATT ATGGCCGTGA CCTCTCCTCT GTGCAAACAC TACTCACCAA ACAGGAAACC 
TTTGATGCTG GACTTCAGGC TTTCCAGCAG GAGGGAATTG CAAACATCAC TGCTCTGAAA 
GACCAGCTAC TGGCAGCCAA ACATATCCAA TCAAAGGCCA TTGAGGTTCG TCACGCTTCC 
TTGATGAAAC GCTGGAATCA GCTGCTTGCT AATTCTGCAG CCAGGAAAAA GAAACTCTTG 
GAGGCTCAGG AGCACTTCAG AAAGGTTGAA GATCTCTTCT TGACTTTTGC CAAGAAGGCC 
TCTGCCTTCA ACAGTTGGTT TGAGAATGCT GAGGAGGACC TGACGGATCC CGTGCGCTGC 
AATTCCCTGG AAGAAATCAA AGCACTGCGA GAAGCCCACG ATGCGTTCCG TTCCTCACTT 
AGCTCTGCCC AAGCTGACTT CAACCAGCTG GCAGAGCTTG ATCGGCAGAT CAAGAGCTTC 
CGTGTAGCCT CCAACCCGTA CACTTGGTTT ACTATGGAGG CTCTTGAAGA AACTTGGAGG 
AATCTGCAGA AAATTATCAA GGAGCGTGAA TTGGAGTTGC AGAAGGAGCA GCGGAGGCAG 
GAAGAAAATG ACAAGTTGCG CCAGGAGTTT GCTCAGCATG CTAATGCCTT CCACCAGTGG 
ATTCAGGAGA CAAGGACTTA CCTGCTAGAT GGGTCCTGCA TGGTGGAGGA GTCGGGAACA 
CTGGAATCCC AGCTGGAAGC TACTAAACGC AAGCACCAGG AGATCCGGGC TATGAGGAGC 
CAGCTGAAGA AGATTGAGGA CCTTGGAGCA GCCATGGAAG AGGCACTTAT CTTGGACAAC 
AAATACACAG AGCACAGCAC CGTGGGGCTG GCGCAGCAGT GGGACCAGCT TGACCAGCTG 
GGAATGAGAA TGCAGCACAA TCTGGAACAG CAGATTCAAG CTCGAAACAC AACTGGAGTC 
ACAGAGGAGG CCCTGAAGGA GTTCAGCATG ATGTTCAAGC ACTTTGACAA GGACAAATCT 
GGACGTCTTA ATCACCAGGA GTTTAAGTCT TGCTTGCGCT CCCTTGGCTA TGACCTGCCC 
ATGGTTGAGG AAGGAGAGCC TGACCCCGAA TTTGAGTCTA TTCTTGACAC TGTTGATCCC 
AACAGGGATG GCCACGTCTC GCTGCAGGAG TACATGGCGT TCATGATCAG CAGGGAAACA 
GAGAACGTGA AATCCAGCGA GGAGATTGAG AGTGCTTTCC GTGCCCTCAG CTCGGAGGGG 
AAGCCTTACG TGACCAAGGA GGAGCTCTAC CAGAACCTGA CCCGGGAGCA GGCCGACTAC 
TGCATTTCTC ACATGAAGCC CTACATGGAT GGCAAGGGCA GAGAACTTCC TTCTGCCTAC 
GACTACATAG AATTTACACG TTCACTCTTT GTGAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015134772.1
CDS	13..7551
Translation	MLSSFRKVKVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSSLRRQKLEDSYRFQFFQRDADELEKWIQEKLQIASDENYKDPSNLQGKLQKHQAFEAEVQANSGAIVKLDETGNQMINEGHFASETIRSFTPYRTAQRPIVTATQVGSHIRTRLQELHRLWELLLEKMREKGVKLLQAQKLVQFLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDLAAHEERVNEVNQFAGKLIQEQHPEEELIKSKQDEVNASWQRLKGLAQQRQGKLFGAAEVQRFNRDVDETISWIKEKGQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPINASQIQVKREELIANWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFRSADESGQALLAAGHYASDEVKEKLTILSDERSALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMDDVATRRDALLSRRNALHERAMKRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYASDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTSVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDADNIKKKQEALVARYEALKDPMVARKQKLADSLRLQQLFRDIEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKIKLNELNQKWDSLKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPTDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTLITKEVGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTNEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVANDLESEGLMAEEVQAVEHQEVYGMMPRDETDSKTASPWKSARMMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAQRLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKEKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLARGHYASPEIKEKLDILDQERTDLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAVLQSFADQLIAADHYAKGVIANRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIEMGNSLIERGACAGSEDAVKARLAALADQWEFLVQKSSEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRETINGRFQRIKSMAAARRAKLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVDHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCANGEDLIKKNNHHVENITAKMKGLKGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHIQSKAIEVRHASLMKRWNQLLANSAARKKKLLEAQEHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFESILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCISHMKPYMDGKGRELPSAYDYIEFTRSLFVN

Target ORF information:

RefSeq Version	XM_015279286.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279286.2

ORF Insert Sequence:

ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG 
TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC 
TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT 
GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT 
GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG 
ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAT CTTTCACGCC TTACAGGACT 
GCACAGCGCC CAATAGTAAC AGCCACCCAA GTTGGGTCCC ACATACGAAC TCGTCTACAG 
GAGCTGCACC GACTATGGGA GTTACTGCTG GAAAAAATGA GAGAGAAGGG AGTGAAGCTG 
TTGCAAGCAC AGAAGCTGGT GCAATTCTTA CGGGAATGTG AAGATGTCAT GGACTGGATC 
AATGACAAGG AAGCAATAGT GACCTCAGAA GAGCTTGGAC AGGACTTAGA GCATGTTGAG 
GTTTTGCAGA AGAAATTTGA AGAGTTCCAA ACAGATCTGG CAGCTCATGA GGAGAGAGTG 
AATGAAGTGA ACCAGTTCGC TGGCAAACTT ATCCAGGAAC AGCACCCTGA GGAGGAACTT 
ATAAAGTCCA AACAGGATGA AGTAAATGCA AGCTGGCAGC GTCTTAAGGG GCTTGCCCAA 
CAGAGGCAAG GAAAACTCTT TGGGGCAGCT GAAGTTCAGC GCTTCAACAG GGATGTGGAT 
GAAACTATCA GCTGGATTAA GGAGAAAGGG CAGTTGATGG CCTCAGATGA CTTTGGCAGA 
GACTTGGCCA GCGTGCAAGC TTTACTACGT AAGCATGAAG GCCTGGAAAG AGATCTTGCA 
GCTTTGGAGG ATAAGGTTAA GGCCCTCTGT GCAGAAGCTG ACCGTTTGCA GCAGTCTCAC 
CCTATAAATG CTTCTCAAAT TCAAGTGAAA CGGGAGGAAC TGATTGCCAA CTGGGAGCAG 
ATCCGAACTC TGGCAGCAGA GAGGCATGCT CGCCTTAATG ACTCCTACAG GTTGCAGCGC 
TTTCTTGCAG ATTTCCGGGA CCTCACTAGC TGGGTAACTG AGATGAAGGC TCTGATAAAT 
GCTGATGAGC TTGCCAATGA TGTGGCTGGA GCAGAAGCCC TTCTAGATAG ACATCAGGAA 
CATAAGGGAG AAATTGATGC TCATGAGGAT AGCTTCAGAT CTGCTGATGA GTCTGGGCAG 
GCTTTGCTTG CAGCTGGGCA CTATGCTTCT GATGAAGTTA AAGAAAAGCT GACTATCCTC 
TCAGATGAAA GATCAGCCTT GCTGGAACTA TGGGAGCTTC GCAGGCAACA GTATGAGCAG 
TGCATGGATC TGCAGCTTTT CTACAGAGAT ACTGAACAAG TTGACAACTG GATGAGCAAA 
CAAGAAGCTT TTCTGCTGAA CGAAGACCTT GGTGATTCTC TGGATAGCGT GGAGGCTCTT 
CTAAAGAAGC ATGAAGATTT TGAGAAATCC CTAAGTGCTC AAGAGGAGAA GATCACAGCA 
CTGGATGAGT TTGCTACTAA ACTGATTCAA AATAACCATT ATGCCATGGA TGACGTTGCT 
ACGCGCAGAG ATGCTCTTCT GAGTCGCCGA AATGCTCTTC ATGAAAGAGC CATGAAGCGC 
CGTGCCCAAC TGGCAGACTC TTTCCATCTC CAGCAGTTTT TCAGGGACTC TGATGAACTG 
AAGAGTTGGG TTAATGAAAA AATGAAAACT GCTACCGATG AGGCTTACAA GGATCCATCC 
AACTTGCAAG GTAAAGTTCA GAAGCATCAG GCTTTTGAAG CAGAGCTTTC TGCTAATCAG 
AGTCGTATTG ATGCACTGGA GAAAGCTGGC CAGAAGTTGA TTGATGTCAA TCACTATGCA 
TCTGATGAGG TGGCAGCTCG CATGAATGAA GTCATCAGCT TGTGGAAGAA ACTTCTGGAA 
GCCACCGAGC TCAAAGGTAT AAAGCTGCGT GAAGCCAATC AACAGCAGCA ATTTAATCGC 
AATGTAGAAG ACATTGAGTT GTGGCTGTAT GAAGTGGAGG GTCACTTGGC TTCTGATGAT 
TATGGAAAAG ATCTTACCAG CGTTCAGAAT CTTCAGAAGA AACATGCCCT GCTAGAGGCA 
GATGTTGCTG CCCATCAGGA TCGGATAGAT GGCATTACCA TCCAGGCACG CCAGTTCCAA 
GATGCTGGGC ACTTTGATGC TGACAATATC AAGAAGAAAC AAGAAGCTTT AGTAGCTCGT 
TATGAAGCTC TGAAGGATCC TATGGTGGCT CGCAAGCAGA AACTTGCAGA TTCTCTTCGC 
CTGCAGCAGC TTTTCCGTGA CATCGAGGAT GAAGAGACCT GGATCAGGGA AAAGGAACCT 
ATTGCAGCCT CAACAAACCG AGGCAAGGAC TTAATTGGTG TCCAGAATCT GCTAAAGAAG 
CACCAGGCTT TGCAGGCAGA AATTGCAGGC CATGAGCCTC GCATTAAAGC AGTCACACAA 
AAAGGAAATG CGATGGTGGA AGAGGGACAT TTTGCTGCTG AAGATGTGAA GATCAAATTG 
AATGAACTAA ACCAAAAATG GGACTCTCTG AAAGCCAAGG CATCTCAACG GCGACAGGAT 
CTAGAGGATT CTCTGCAAGC TCAGCAGTAT TTTGCTGATG CTAATGAGGC AGAATCATGG 
ATGAGGGAAA AGGAGCCCAT TGTAGGCAGC ACGGATTATG GAAAGGATGA AGACTCTGCT 
GAGGCTCTCC TGAAGAAGCA TGAAGCTTTG ATGTCTGATC TTTCTGCTTA CGGCAGTAGC 
ATACAGGCAT TAAGGGAACA GGCCCAGTCC TGCAGGCAAC AAGTTGCTCC CACTGATGAT 
GAAACTGGAA AAGAGCTTGT GCTAGCACTC TATGATTACC AAGAGAAGAG TCCTCGGGAG 
GTGACTATGA AGAAAGGAGA TATTCTAACC CTGCTCAACA GCACCAACAA GGACTGGTGG 
AAGGTTGAAG TTAACGATCG TCAGGGCTTT GTACCAGCTG CCTATGTGAA AAAACTAGAT 
CCTGCCCAGT CTGCATCCCG AGAGAATCTT CTAGAAGAGC AAGGCAGCAT AGCATTGCGA 
CAGGAGCAAA TTGACAACCA GACTCTCATT ACTAAGGAAG TCGGCAGTGT ATCTCTGCGT 
ATGAAACAGG TCGAAGAACT GTATCATTCC CTTCTTGAGC TGGGAGAAAA ACGTAAAGGC 
ATGCTAGAAA AAAGCTGCAA GAAGTTTATG CTTTTCCGTG AGGCTAATGA GCTTCAACAG 
TGGATCAATG AGAAGGAAGC TGCGCTCACT AATGAGGAAG TGGGTGCTGA TTTGGAGCAG 
GTGGAGGTGC TACAGAAGAA GTTTGATGAT TTTCAGAAGG ATTTAAAAGC TAATGAGTCA 
CGACTGAAGG ACATAAACAA GGTTGCAAAT GACCTGGAGT CTGAAGGGCT GATGGCAGAG 
GAAGTGCAAG CAGTAGAGCA CCAGGAAGTC TATGGAATGA TGCCCAGAGA TGAAACTGAT 
TCTAAGACAG CCTCTCCTTG GAAGTCTGCA CGTATGATGG TACACACAGT GGCAACGTTT 
AACTCAATCA AGGAACTGAA TGAACGCTGG AGATCCCTGC AGCAGTTGGC AGAGGAGCGA 
AGCCAGCTGT TGGGCAGCGC TCATGAAGTC CAGAGGTTCC ACAGAGATGC TGATGAAACA 
AAAGAATGGA TAGAAGAGAA GAATCAAGCA TTAAATACCG ACAACTATGG ACATGACTTG 
GCCAGTGTGC AGGCTCTGCA GCGCAAGCAT GAAGGCTTTG AGAGAGACTT GGCAGCTCTT 
GGAGACAAGG TGAATTCTCT TGGTGAAACT GCCCAGCGTC TGATCCAGTC ACATCCAGAA 
TCAGCTGAAG ATCTTCAAGA AAAATGTACT GAGTTGAACC AAGCCTGGAG TAGTCTGGGA 
AAACGTGCTG ACCAACGCAA AGAGAAGCTT GGAGATTCTC ATGACCTGCA GCGTTTCCTC 
AGTGATTTTA GAGACCTTAT GTCTTGGATC AATGGAATCC GGGGTCTGGT CTCCTCAGAT 
GAACTTGCAA AAGATGTGAC TGGAGCTGAA GCTCTGTTGG AAAGGCATCA GGAGCACCGT 
ACTGAAATAG ATGCACGGGC TGGCACTTTT CAGGCATTTG AACAGTTTGG ACAGCAGCTT 
CTGGCTCGTG GACACTATGC CAGCCCAGAG ATTAAGGAGA AACTGGATAT TCTAGATCAA 
GAACGGACAG ACCTAGAGAA GGCCTGGGTC CAGCGCAGAA TGATGCTAGA CCAGTGCTTA 
GAACTACAGC TGTTTCATCG GGATTGTGAA CAAGCTGAAA ACTGGATGGC TGCCCGAGAG 
GCGTTCCTAA ATACAGAAGA CAAAGGAGAC TCCTTAGACA GCGTGGAGGC ACTCATCAAG 
AAGCATGAAG ATTTCGATAA AGCAATCAAT GTCCAGGAAG AGAAAATTGC TGTCTTGCAG 
TCTTTTGCTG ACCAACTGAT TGCTGCTGAT CATTATGCAA AAGGAGTCAT TGCTAACAGA 
CGCAATGAGG TTCTGGACAG GTGGCGTCGT CTGAAGGCTC AGATGATTGA GAAGAGATCT 
AAGCTGGGAG AATCTCAGAC CCTCCAACAG TTCAGTCGTG ATGTTGATGA AATAGAAGCT 
TGGATCAGTG AAAAGCTTCA AACTGCAAGT GATGAGTCAT ATAAGGATCC CACAAACATC 
CAGCTTTCCA AACTGCTGAG CAAGCACCAG AAGCACCAAG CCTTTGAAGC TGAGCTCCAC 
GCCAACGCAG ATCGGATTCG TGGAGTCATT GAAATGGGGA ACTCTCTTAT TGAAAGAGGA 
GCGTGTGCTG GCAGCGAGGA TGCTGTGAAG GCTCGGCTGG CTGCCCTGGC TGACCAATGG 
GAGTTCCTGG TCCAGAAGTC ATCAGAGAAG AGTCAGAAAC TGAAAGAAGC AAATAAACAG 
CAGAATTTCA ATACCGGCAT CAAGGACTTT GATTTCTGGC TTTCAGAGGT GGAAGCTTTG 
TTGGCATCTG AAGACTATGG GAAGGACTTG GCATCAGTGA ACAACCTTCT GAAGAAGCAC 
CAATTACTGG AAGCTGATAT ATCTGCTCAT GAGGATCGGC TGAAGGACCT GAACAGCCAG 
GCTGACAGTT TGATGACCAG CAGTGCTTTC GATACCTCCC AAGTAAAGGA TAAACGTGAA 
ACTATAAATG GGCGCTTCCA GAGAATCAAG AGCATGGCAG CTGCCCGCCG TGCGAAGCTC 
AACGAGTCGC ACCGCTTGCA TCAGTTCTTC CGTGACATGG ATGATGAGGA GTCCTGGATC 
AAAGAGAAGA AACTGTTGGT GAGCTCAGAG GACTATGGCA GAGACCTGAC TGGTGTGCAG 
AACCTGAGGA AGAAACATAA GCGCTTGGAA GCAGAATTAG CTGCCCATGA ACCTGCTATC 
CAGGGTGTTC TAGACACTGG CAAGAAGCTT TCAGATGATA ACACAATTGG GAAGGAGGAG 
ATACAGCAAA GACTGGCTCA GTTTGTGGAC CACTGGAAAG AGTTAAAACA GCTGGCAGCT 
GCTCGGGGGC AGCGTCTGGA GGAGTCCCTG GAGTACCAAC AGTTTGTAGC AAATGTTGAG 
GAAGAGGAGG CATGGATCAA CGAGAAAATG ACATTGGTAG CCAGTGAGGA TTATGGAGAC 
ACACTTGCTG CTATCCAGGG CTTGCTGAAG AAGCATGAAG CATTCGAGAC TGACTTTACT 
GTCCACAAAG ACAGAGTGAA TGATGTTTGC GCTAATGGAG AGGATCTCAT TAAAAAGAAT 
AATCACCATG TGGAGAACAT TACTGCTAAG ATGAAGGGCC TCAAGGGCAA GGTATCAGAT 
CTGGAGAAAG CCGCAGCTCA GAGGAAAGCC AAACTGGATG AGAACTCTGC CTTCCTCCAG 
TTCAACTGGA AAGCAGATGT GGTGGAGTCA TGGATAGGTG AGAAGGAAAA CAGTCTGAAG 
ACAGATGATT ATGGCCGTGA CCTCTCCTCT GTGCAAACAC TACTCACCAA ACAGGAAACC 
TTTGATGCTG GACTTCAGGC TTTCCAGCAG GAGGGAATTG CAAACATCAC TGCTCTGAAA 
GACCAGCTAC TGGCAGCCAA ACATATCCAA TCAAAGGCCA TTGAGGTTCG TCACGCTTCC 
TTGATGAAAC GCTGGAATCA GCTGCTTGCT AATTCTGCAG CCAGGAAAAA GAAACTCTTG 
GAGGCTCAGG AGCACTTCAG AAAGGTTGAA GATCTCTTCT TGACTTTTGC CAAGAAGGCC 
TCTGCCTTCA ACAGTTGGTT TGAGAATGCT GAGGAGGACC TGACGGATCC CGTGCGCTGC 
AATTCCCTGG AAGAAATCAA AGCACTGCGA GAAGCCCACG ATGCGTTCCG TTCCTCACTT 
AGCTCTGCCC AAGCTGACTT CAACCAGCTG GCAGAGCTTG ATCGGCAGAT CAAGAGCTTC 
CGTGTAGCCT CCAACCCGTA CACTTGGTTT ACTATGGAGG CTCTTGAAGA AACTTGGAGG 
AATCTGCAGA AAATTATCAA GGAGCGTGAA TTGGAGTTGC AGAAGGAGCA GCGGAGGCAG 
GAAGAAAATG ACAAGTTGCG CCAGGAGTTT GCTCAGCATG CTAATGCCTT CCACCAGTGG 
ATTCAGGAGA CAAGGACTTA CCTGCTAGAT GGGTCCTGCA TGGTGGAGGA GTCGGGAACA 
CTGGAATCCC AGCTGGAAGC TACTAAACGC AAGCACCAGG AGATCCGGGC TATGAGGAGC 
CAGCTGAAGA AGATTGAGGA CCTTGGAGCA GCCATGGAAG AGGCACTTAT CTTGGACAAC 
AAATACACAG AGCACAGCAC CGTGGGGCTG GCGCAGCAGT GGGACCAGCT TGACCAGCTG 
GGAATGAGAA TGCAGCACAA TCTGGAACAG CAGATTCAAG CTCGAAACAC AACTGGAGTC 
ACAGAGGAGG CCCTGAAGGA GTTCAGCATG ATGTTCAAGC ACTTTGACAA GGACAAATCT 
GGACGTCTTA ATCACCAGGA GTTTAAGTCT TGCTTGCGCT CCCTTGGCTA TGACCTGCCC 
ATGGTTGAGG AAGGAGAGCC TGACCCCGAA TTTGAGTCTA TTCTTGACAC TGTTGATCCC 
AACAGGGATG GCCACGTCTC GCTGCAGGAG TACATGGCGT TCATGATCAG CAGGGAAACA 
GAGAACGTGA AATCCAGCGA GGAGATTGAG AGTGCTTTCC GTGCCCTCAG CTCGGAGGGG 
AAGCCTTACG TGACCAAGGA GGAGCTCTAC CAGAACCTGA CCCGGGAGCA GGCCGACTAC 
TGCATTTCTC ACATGAAGCC CTACATGGAT GGCAAGGGCA GAGAACTTCC TTCTGCCTAC 
GACTACATAG AATTTACACG TTCACTCTTT GTGAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_015279287.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015134773.1 Gallus gallus spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X6, mRNA.

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CloneID	OGa43313
Clone ID Related Accession (Same CDS sequence)	XM_015279287.1 , XM_015279287.2
Accession Version	XM_015279287.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7533bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG 
TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC 
TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT 
GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT 
GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG 
ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAA CTCGTCTACA GGAGCTGCAC 
CGACTATGGG AGTTACTGCT GGAAAAAATG AGAGAGAAGG GAGTGAAGCT GTTGCAAGCA 
CAGAAGCTGG TGCAATTCTT ACGGGAATGT GAAGATGTCA TGGACTGGAT CAATGACAAG 
GAAGCAATAG TGACCTCAGA AGAGCTTGGA CAGGACTTAG AGCATGTTGA GGTTTTGCAG 
AAGAAATTTG AAGAGTTCCA AACAGATCTG GCAGCTCATG AGGAGAGAGT GAATGAAGTG 
AACCAGTTCG CTGGCAAACT TATCCAGGAA CAGCACCCTG AGGAGGAACT TATAAAGTCC 
AAACAGGATG AAGTAAATGC AAGCTGGCAG CGTCTTAAGG GGCTTGCCCA ACAGAGGCAA 
GGAAAACTCT TTGGGGCAGC TGAAGTTCAG CGCTTCAACA GGGATGTGGA TGAAACTATC 
AGCTGGATTA AGGAGAAAGG GCAGTTGATG GCCTCAGATG ACTTTGGCAG AGACTTGGCC 
AGCGTGCAAG CTTTACTACG TAAGCATGAA GGCCTGGAAA GAGATCTTGC AGCTTTGGAG 
GATAAGGTTA AGGCCCTCTG TGCAGAAGCT GACCGTTTGC AGCAGTCTCA CCCTATAAAT 
GCTTCTCAAA TTCAAGTGAA ACGGGAGGAA CTGATTGCCA ACTGGGAGCA GATCCGAACT 
CTGGCAGCAG AGAGGCATGC TCGCCTTAAT GACTCCTACA GGTTGCAGCG CTTTCTTGCA 
GATTTCCGGG ACCTCACTAG CTGGGTAACT GAGATGAAGG CTCTGATAAA TGCTGATGAG 
CTTGCCAATG ATGTGGCTGG AGCAGAAGCC CTTCTAGATA GACATCAGGA ACATAAGGGA 
GAAATTGATG CTCATGAGGA TAGCTTCAGA TCTGCTGATG AGTCTGGGCA GGCTTTGCTT 
GCAGCTGGGC ACTATGCTTC TGATGAAGTT AAAGAAAAGC TGACTATCCT CTCAGATGAA 
AGATCAGCCT TGCTGGAACT ATGGGAGCTT CGCAGGCAAC AGTATGAGCA GTGCATGGAT 
CTGCAGCTTT TCTACAGAGA TACTGAACAA GTTGACAACT GGATGAGCAA ACAAGAAGCT 
TTTCTGCTGA ACGAAGACCT TGGTGATTCT CTGGATAGCG TGGAGGCTCT TCTAAAGAAG 
CATGAAGATT TTGAGAAATC CCTAAGTGCT CAAGAGGAGA AGATCACAGC ACTGGATGAG 
TTTGCTACTA AACTGATTCA AAATAACCAT TATGCCATGG ATGACGTTGC TACGCGCAGA 
GATGCTCTTC TGAGTCGCCG AAATGCTCTT CATGAAAGAG CCATGAAGCG CCGTGCCCAA 
CTGGCAGACT CTTTCCATCT CCAGCAGTTT TTCAGGGACT CTGATGAACT GAAGAGTTGG 
GTTAATGAAA AAATGAAAAC TGCTACCGAT GAGGCTTACA AGGATCCATC CAACTTGCAA 
GGTAAAGTTC AGAAGCATCA GGCTTTTGAA GCAGAGCTTT CTGCTAATCA GAGTCGTATT 
GATGCACTGG AGAAAGCTGG CCAGAAGTTG ATTGATGTCA ATCACTATGC ATCTGATGAG 
GTGGCAGCTC GCATGAATGA AGTCATCAGC TTGTGGAAGA AACTTCTGGA AGCCACCGAG 
CTCAAAGGTA TAAAGCTGCG TGAAGCCAAT CAACAGCAGC AATTTAATCG CAATGTAGAA 
GACATTGAGT TGTGGCTGTA TGAAGTGGAG GGTCACTTGG CTTCTGATGA TTATGGAAAA 
GATCTTACCA GCGTTCAGAA TCTTCAGAAG AAACATGCCC TGCTAGAGGC AGATGTTGCT 
GCCCATCAGG ATCGGATAGA TGGCATTACC ATCCAGGCAC GCCAGTTCCA AGATGCTGGG 
CACTTTGATG CTGACAATAT CAAGAAGAAA CAAGAAGCTT TAGTAGCTCG TTATGAAGCT 
CTGAAGGATC CTATGGTGGC TCGCAAGCAG AAACTTGCAG ATTCTCTTCG CCTGCAGCAG 
CTTTTCCGTG ACATCGAGGA TGAAGAGACC TGGATCAGGG AAAAGGAACC TATTGCAGCC 
TCAACAAACC GAGGCAAGGA CTTAATTGGT GTCCAGAATC TGCTAAAGAA GCACCAGGCT 
TTGCAGGCAG AAATTGCAGG CCATGAGCCT CGCATTAAAG CAGTCACACA AAAAGGAAAT 
GCGATGGTGG AAGAGGGACA TTTTGCTGCT GAAGATGTGA AGATCAAATT GAATGAACTA 
AACCAAAAAT GGGACTCTCT GAAAGCCAAG GCATCTCAAC GGCGACAGGA TCTAGAGGAT 
TCTCTGCAAG CTCAGCAGTA TTTTGCTGAT GCTAATGAGG CAGAATCATG GATGAGGGAA 
AAGGAGCCCA TTGTAGGCAG CACGGATTAT GGAAAGGATG AAGACTCTGC TGAGGCTCTC 
CTGAAGAAGC ATGAAGCTTT GATGTCTGAT CTTTCTGCTT ACGGCAGTAG CATACAGGCA 
TTAAGGGAAC AGGCCCAGTC CTGCAGGCAA CAAGTTGCTC CCACTGATGA TGAAACTGGA 
AAAGAGCTTG TGCTAGCACT CTATGATTAC CAAGAGAAGA GTCCTCGGGA GGTGACTATG 
AAGAAAGGAG ATATTCTAAC CCTGCTCAAC AGCACCAACA AGGACTGGTG GAAGGTTGAA 
GTTAACGATC GTCAGGGCTT TGTACCAGCT GCCTATGTGA AAAAACTAGA TCCTGCCCAG 
TCTGCATCCC GAGAGAATCT TCTAGAAGAG CAAGGCAGCA TAGCATTGCG ACAGGAGCAA 
ATTGACAACC AGACTCTCAT TACTAAGGAA GTCGGCAGTG TATCTCTGCG TATGAAACAG 
GTCGAAGAAC TGTATCATTC CCTTCTTGAG CTGGGAGAAA AACGTAAAGG CATGCTAGAA 
AAAAGCTGCA AGAAGTTTAT GCTTTTCCGT GAGGCTAATG AGCTTCAACA GTGGATCAAT 
GAGAAGGAAG CTGCGCTCAC TAATGAGGAA GTGGGTGCTG ATTTGGAGCA GGTGGAGGTG 
CTACAGAAGA AGTTTGATGA TTTTCAGAAG GATTTAAAAG CTAATGAGTC ACGACTGAAG 
GACATAAACA AGGTTGCAAA TGACCTGGAG TCTGAAGGGC TGATGGCAGA GGAAGTGCAA 
GCAGTAGAGC ACCAGGAAGT CTATGGAATG ATGCCCAGAG ATGAAACTGA TTCTAAGACA 
GCCTCTCCTT GGAAGTCTGC ACGTATGATG GTACACACAG TGGCAACGTT TAACTCAATC 
AAGGAACTGA ATGAACGCTG GAGATCCCTG CAGCAGTTGG CAGAGGAGCG AAGCCAGCTG 
TTGGGCAGCG CTCATGAAGT CCAGAGGTTC CACAGAGATG CTGATGAAAC AAAAGAATGG 
ATAGAAGAGA AGAATCAAGC ATTAAATACC GACAACTATG GACATGACTT GGCCAGTGTG 
CAGGCTCTGC AGCGCAAGCA TGAAGGCTTT GAGAGAGACT TGGCAGCTCT TGGAGACAAG 
GTGAATTCTC TTGGTGAAAC TGCCCAGCGT CTGATCCAGT CACATCCAGA ATCAGCTGAA 
GATCTTCAAG AAAAATGTAC TGAGTTGAAC CAAGCCTGGA GTAGTCTGGG AAAACGTGCT 
GACCAACGCA AAGAGAAGCT TGGAGATTCT CATGACCTGC AGCGTTTCCT CAGTGATTTT 
AGAGACCTTA TGTCTTGGAT CAATGGAATC CGGGGTCTGG TCTCCTCAGA TGAACTTGCA 
AAAGATGTGA CTGGAGCTGA AGCTCTGTTG GAAAGGCATC AGGAGCACCG TACTGAAATA 
GATGCACGGG CTGGCACTTT TCAGGCATTT GAACAGTTTG GACAGCAGCT TCTGGCTCGT 
GGACACTATG CCAGCCCAGA GATTAAGGAG AAACTGGATA TTCTAGATCA AGAACGGACA 
GACCTAGAGA AGGCCTGGGT CCAGCGCAGA ATGATGCTAG ACCAGTGCTT AGAACTACAG 
CTGTTTCATC GGGATTGTGA ACAAGCTGAA AACTGGATGG CTGCCCGAGA GGCGTTCCTA 
AATACAGAAG ACAAAGGAGA CTCCTTAGAC AGCGTGGAGG CACTCATCAA GAAGCATGAA 
GATTTCGATA AAGCAATCAA TGTCCAGGAA GAGAAAATTG CTGTCTTGCA GTCTTTTGCT 
GACCAACTGA TTGCTGCTGA TCATTATGCA AAAGGAGTCA TTGCTAACAG ACGCAATGAG 
GTTCTGGACA GGTGGCGTCG TCTGAAGGCT CAGATGATTG AGAAGAGATC TAAGCTGGGA 
GAATCTCAGA CCCTCCAACA GTTCAGTCGT GATGTTGATG AAATAGAAGC TTGGATCAGT 
GAAAAGCTTC AAACTGCAAG TGATGAGTCA TATAAGGATC CCACAAACAT CCAGCTTTCC 
AAACTGCTGA GCAAGCACCA GAAGCACCAA GCCTTTGAAG CTGAGCTCCA CGCCAACGCA 
GATCGGATTC GTGGAGTCAT TGAAATGGGG AACTCTCTTA TTGAAAGAGG AGCGTGTGCT 
GGCAGCGAGG ATGCTGTGAA GGCTCGGCTG GCTGCCCTGG CTGACCAATG GGAGTTCCTG 
GTCCAGAAGT CATCAGAGAA GAGTCAGAAA CTGAAAGAAG CAAATAAACA GCAGAATTTC 
AATACCGGCA TCAAGGACTT TGATTTCTGG CTTTCAGAGG TGGAAGCTTT GTTGGCATCT 
GAAGACTATG GGAAGGACTT GGCATCAGTG AACAACCTTC TGAAGAAGCA CCAATTACTG 
GAAGCTGATA TATCTGCTCA TGAGGATCGG CTGAAGGACC TGAACAGCCA GGCTGACAGT 
TTGATGACCA GCAGTGCTTT CGATACCTCC CAAGTAAAGG ATAAACGTGA AACTATAAAT 
GGGCGCTTCC AGAGAATCAA GAGCATGGCA GCTGCCCGCC GTGCGAAGCT CAACGAGTCG 
CACCGCTTGC ATCAGTTCTT CCGTGACATG GATGATGAGG AGTCCTGGAT CAAAGAGAAG 
AAACTGTTGG TGAGCTCAGA GGACTATGGC AGAGACCTGA CTGGTGTGCA GAACCTGAGG 
AAGAAACATA AGCGCTTGGA AGCAGAATTA GCTGCCCATG AACCTGCTAT CCAGGGTGTT 
CTAGACACTG GCAAGAAGCT TTCAGATGAT AACACAATTG GGAAGGAGGA GATACAGCAA 
AGACTGGCTC AGTTTGTGGA CCACTGGAAA GAGTTAAAAC AGCTGGCAGC TGCTCGGGGG 
CAGCGTCTGG AGGAGTCCCT GGAGTACCAA CAGTTTGTAG CAAATGTTGA GGAAGAGGAG 
GCATGGATCA ACGAGAAAAT GACATTGGTA GCCAGTGAGG ATTATGGAGA CACACTTGCT 
GCTATCCAGG GCTTGCTGAA GAAGCATGAA GCATTCGAGA CTGACTTTAC TGTCCACAAA 
GACAGAGTGA ATGATGTTTG CGCTAATGGA GAGGATCTCA TTAAAAAGAA TAATCACCAT 
GTGGAGAACA TTACTGCTAA GATGAAGGGC CTCAAGGGCA AGGTATCAGA TCTGGAGAAA 
GCCGCAGCTC AGAGGAAAGC CAAACTGGAT GAGAACTCTG CCTTCCTCCA GTTCAACTGG 
AAAGCAGATG TGGTGGAGTC ATGGATAGGT GAGAAGGAAA ACAGTCTGAA GACAGATGAT 
TATGGCCGTG ACCTCTCCTC TGTGCAAACA CTACTCACCA AACAGGAAAC CTTTGATGCT 
GGACTTCAGG CTTTCCAGCA GGAGGGAATT GCAAACATCA CTGCTCTGAA AGACCAGCTA 
CTGGCAGCCA AACATATCCA ATCAAAGGCC ATTGAGGTTC GTCACGCTTC CTTGATGAAA 
CGCTGGAATC AGCTGCTTGC TAATTCTGCA GCCAGGAAAA AGAAACTCTT GGAGGCTCAG 
GAGCACTTCA GAAAGGTTGA AGATCTCTTC TTGACTTTTG CCAAGAAGGC CTCTGCCTTC 
AACAGTTGGT TTGAGAATGC TGAGGAGGAC CTGACGGATC CCGTGCGCTG CAATTCCCTG 
GAAGAAATCA AAGCACTGCG AGAAGCCCAC GATGCGTTCC GTTCCTCACT TAGCTCTGCC 
CAAGCTGACT TCAACCAGCT GGCAGAGCTT GATCGGCAGA TCAAGAGCTT CCGTGTAGCC 
TCCAACCCGT ACACTTGGTT TACTATGGAG GCTCTTGAAG AAACTTGGAG GAATCTGCAG 
AAAATTATCA AGGAGCGTGA ATTGGAGTTG CAGAAGGAGC AGCGGAGGCA GGAAGAAAAT 
GACAAGTTGC GCCAGGAGTT TGCTCAGCAT GCTAATGCCT TCCACCAGTG GATTCAGGAG 
ACAAGGACTT ACCTGCTAGA TGGCATAGCC TATCGTCGTG TCATTCGTGT CTATCAGTAT 
GAAGTTGGGG ATGATCTATC TGGAAGGTCC TGCATGGTGG AGGAGTCGGG AACACTGGAA 
TCCCAGCTGG AAGCTACTAA ACGCAAGCAC CAGGAGATCC GGGCTATGAG GAGCCAGCTG 
AAGAAGATTG AGGACCTTGG AGCAGCCATG GAAGAGGCAC TTATCTTGGA CAACAAATAC 
ACAGAGCACA GCACCGTGGG GCTGGCGCAG CAGTGGGACC AGCTTGACCA GCTGGGAATG 
AGAATGCAGC ACAATCTGGA ACAGCAGATT CAAGCTCGAA ACACAACTGG AGTCACAGAG 
GAGGCCCTGA AGGAGTTCAG CATGATGTTC AAGCACTTTG ACAAGGACAA ATCTGGACGT 
CTTAATCACC AGGAGTTTAA GTCTTGCTTG CGCTCCCTTG GCTATGACCT GCCCATGGTT 
GAGGAAGGAG AGCCTGACCC CGAATTTGAG TCTATTCTTG ACACTGTTGA TCCCAACAGG 
GATGGCCACG TCTCGCTGCA GGAGTACATG GCGTTCATGA TCAGCAGGGA AACAGAGAAC 
GTGAAATCCA GCGAGGAGAT TGAGAGTGCT TTCCGTGCCC TCAGCTCGGA GGGGAAGCCT 
TACGTGACCA AGGAGGAGCT CTACCAGAAC CTGACCCGGG AGCAGGCCGA CTACTGCATT 
TCTCACATGA AGCCCTACAT GGATGGCAAG GGCAGAGAAC TTCCTTCTGC CTACGACTAC 
ATAGAATTTA CACGTTCACT CTTTGTGAAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015134773.1
CDS	16..7548
Translation	MLSSFRKVKVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSSLRRQKLEDSYRFQFFQRDADELEKWIQEKLQIASDENYKDPSNLQGKLQKHQAFEAEVQANSGAIVKLDETGNQMINEGHFASETIRTRLQELHRLWELLLEKMREKGVKLLQAQKLVQFLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDLAAHEERVNEVNQFAGKLIQEQHPEEELIKSKQDEVNASWQRLKGLAQQRQGKLFGAAEVQRFNRDVDETISWIKEKGQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPINASQIQVKREELIANWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFRSADESGQALLAAGHYASDEVKEKLTILSDERSALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMDDVATRRDALLSRRNALHERAMKRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYASDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTSVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDADNIKKKQEALVARYEALKDPMVARKQKLADSLRLQQLFRDIEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKIKLNELNQKWDSLKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPTDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTLITKEVGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTNEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVANDLESEGLMAEEVQAVEHQEVYGMMPRDETDSKTASPWKSARMMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAQRLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKEKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLARGHYASPEIKEKLDILDQERTDLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAVLQSFADQLIAADHYAKGVIANRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIEMGNSLIERGACAGSEDAVKARLAALADQWEFLVQKSSEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRETINGRFQRIKSMAAARRAKLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVDHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCANGEDLIKKNNHHVENITAKMKGLKGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHIQSKAIEVRHASLMKRWNQLLANSAARKKKLLEAQEHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGIAYRRVIRVYQYEVGDDLSGRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFESILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCISHMKPYMDGKGRELPSAYDYIEFTRSLFVN

Target ORF information:

RefSeq Version	XM_015279287.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279287.1

ORF Insert Sequence:

ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG 
TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC 
TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT 
GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT 
GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG 
ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAA CTCGTCTACA GGAGCTGCAC 
CGACTATGGG AGTTACTGCT GGAAAAAATG AGAGAGAAGG GAGTGAAGCT GTTGCAAGCA 
CAGAAGCTGG TGCAATTCTT ACGGGAATGT GAAGATGTCA TGGACTGGAT CAATGACAAG 
GAAGCAATAG TGACCTCAGA AGAGCTTGGA CAGGACTTAG AGCATGTTGA GGTTTTGCAG 
AAGAAATTTG AAGAGTTCCA AACAGATCTG GCAGCTCATG AGGAGAGAGT GAATGAAGTG 
AACCAGTTCG CTGGCAAACT TATCCAGGAA CAGCACCCTG AGGAGGAACT TATAAAGTCC 
AAACAGGATG AAGTAAATGC AAGCTGGCAG CGTCTTAAGG GGCTTGCCCA ACAGAGGCAA 
GGAAAACTCT TTGGGGCAGC TGAAGTTCAG CGCTTCAACA GGGATGTGGA TGAAACTATC 
AGCTGGATTA AGGAGAAAGG GCAGTTGATG GCCTCAGATG ACTTTGGCAG AGACTTGGCC 
AGCGTGCAAG CTTTACTACG TAAGCATGAA GGCCTGGAAA GAGATCTTGC AGCTTTGGAG 
GATAAGGTTA AGGCCCTCTG TGCAGAAGCT GACCGTTTGC AGCAGTCTCA CCCTATAAAT 
GCTTCTCAAA TTCAAGTGAA ACGGGAGGAA CTGATTGCCA ACTGGGAGCA GATCCGAACT 
CTGGCAGCAG AGAGGCATGC TCGCCTTAAT GACTCCTACA GGTTGCAGCG CTTTCTTGCA 
GATTTCCGGG ACCTCACTAG CTGGGTAACT GAGATGAAGG CTCTGATAAA TGCTGATGAG 
CTTGCCAATG ATGTGGCTGG AGCAGAAGCC CTTCTAGATA GACATCAGGA ACATAAGGGA 
GAAATTGATG CTCATGAGGA TAGCTTCAGA TCTGCTGATG AGTCTGGGCA GGCTTTGCTT 
GCAGCTGGGC ACTATGCTTC TGATGAAGTT AAAGAAAAGC TGACTATCCT CTCAGATGAA 
AGATCAGCCT TGCTGGAACT ATGGGAGCTT CGCAGGCAAC AGTATGAGCA GTGCATGGAT 
CTGCAGCTTT TCTACAGAGA TACTGAACAA GTTGACAACT GGATGAGCAA ACAAGAAGCT 
TTTCTGCTGA ACGAAGACCT TGGTGATTCT CTGGATAGCG TGGAGGCTCT TCTAAAGAAG 
CATGAAGATT TTGAGAAATC CCTAAGTGCT CAAGAGGAGA AGATCACAGC ACTGGATGAG 
TTTGCTACTA AACTGATTCA AAATAACCAT TATGCCATGG ATGACGTTGC TACGCGCAGA 
GATGCTCTTC TGAGTCGCCG AAATGCTCTT CATGAAAGAG CCATGAAGCG CCGTGCCCAA 
CTGGCAGACT CTTTCCATCT CCAGCAGTTT TTCAGGGACT CTGATGAACT GAAGAGTTGG 
GTTAATGAAA AAATGAAAAC TGCTACCGAT GAGGCTTACA AGGATCCATC CAACTTGCAA 
GGTAAAGTTC AGAAGCATCA GGCTTTTGAA GCAGAGCTTT CTGCTAATCA GAGTCGTATT 
GATGCACTGG AGAAAGCTGG CCAGAAGTTG ATTGATGTCA ATCACTATGC ATCTGATGAG 
GTGGCAGCTC GCATGAATGA AGTCATCAGC TTGTGGAAGA AACTTCTGGA AGCCACCGAG 
CTCAAAGGTA TAAAGCTGCG TGAAGCCAAT CAACAGCAGC AATTTAATCG CAATGTAGAA 
GACATTGAGT TGTGGCTGTA TGAAGTGGAG GGTCACTTGG CTTCTGATGA TTATGGAAAA 
GATCTTACCA GCGTTCAGAA TCTTCAGAAG AAACATGCCC TGCTAGAGGC AGATGTTGCT 
GCCCATCAGG ATCGGATAGA TGGCATTACC ATCCAGGCAC GCCAGTTCCA AGATGCTGGG 
CACTTTGATG CTGACAATAT CAAGAAGAAA CAAGAAGCTT TAGTAGCTCG TTATGAAGCT 
CTGAAGGATC CTATGGTGGC TCGCAAGCAG AAACTTGCAG ATTCTCTTCG CCTGCAGCAG 
CTTTTCCGTG ACATCGAGGA TGAAGAGACC TGGATCAGGG AAAAGGAACC TATTGCAGCC 
TCAACAAACC GAGGCAAGGA CTTAATTGGT GTCCAGAATC TGCTAAAGAA GCACCAGGCT 
TTGCAGGCAG AAATTGCAGG CCATGAGCCT CGCATTAAAG CAGTCACACA AAAAGGAAAT 
GCGATGGTGG AAGAGGGACA TTTTGCTGCT GAAGATGTGA AGATCAAATT GAATGAACTA 
AACCAAAAAT GGGACTCTCT GAAAGCCAAG GCATCTCAAC GGCGACAGGA TCTAGAGGAT 
TCTCTGCAAG CTCAGCAGTA TTTTGCTGAT GCTAATGAGG CAGAATCATG GATGAGGGAA 
AAGGAGCCCA TTGTAGGCAG CACGGATTAT GGAAAGGATG AAGACTCTGC TGAGGCTCTC 
CTGAAGAAGC ATGAAGCTTT GATGTCTGAT CTTTCTGCTT ACGGCAGTAG CATACAGGCA 
TTAAGGGAAC AGGCCCAGTC CTGCAGGCAA CAAGTTGCTC CCACTGATGA TGAAACTGGA 
AAAGAGCTTG TGCTAGCACT CTATGATTAC CAAGAGAAGA GTCCTCGGGA GGTGACTATG 
AAGAAAGGAG ATATTCTAAC CCTGCTCAAC AGCACCAACA AGGACTGGTG GAAGGTTGAA 
GTTAACGATC GTCAGGGCTT TGTACCAGCT GCCTATGTGA AAAAACTAGA TCCTGCCCAG 
TCTGCATCCC GAGAGAATCT TCTAGAAGAG CAAGGCAGCA TAGCATTGCG ACAGGAGCAA 
ATTGACAACC AGACTCTCAT TACTAAGGAA GTCGGCAGTG TATCTCTGCG TATGAAACAG 
GTCGAAGAAC TGTATCATTC CCTTCTTGAG CTGGGAGAAA AACGTAAAGG CATGCTAGAA 
AAAAGCTGCA AGAAGTTTAT GCTTTTCCGT GAGGCTAATG AGCTTCAACA GTGGATCAAT 
GAGAAGGAAG CTGCGCTCAC TAATGAGGAA GTGGGTGCTG ATTTGGAGCA GGTGGAGGTG 
CTACAGAAGA AGTTTGATGA TTTTCAGAAG GATTTAAAAG CTAATGAGTC ACGACTGAAG 
GACATAAACA AGGTTGCAAA TGACCTGGAG TCTGAAGGGC TGATGGCAGA GGAAGTGCAA 
GCAGTAGAGC ACCAGGAAGT CTATGGAATG ATGCCCAGAG ATGAAACTGA TTCTAAGACA 
GCCTCTCCTT GGAAGTCTGC ACGTATGATG GTACACACAG TGGCAACGTT TAACTCAATC 
AAGGAACTGA ATGAACGCTG GAGATCCCTG CAGCAGTTGG CAGAGGAGCG AAGCCAGCTG 
TTGGGCAGCG CTCATGAAGT CCAGAGGTTC CACAGAGATG CTGATGAAAC AAAAGAATGG 
ATAGAAGAGA AGAATCAAGC ATTAAATACC GACAACTATG GACATGACTT GGCCAGTGTG 
CAGGCTCTGC AGCGCAAGCA TGAAGGCTTT GAGAGAGACT TGGCAGCTCT TGGAGACAAG 
GTGAATTCTC TTGGTGAAAC TGCCCAGCGT CTGATCCAGT CACATCCAGA ATCAGCTGAA 
GATCTTCAAG AAAAATGTAC TGAGTTGAAC CAAGCCTGGA GTAGTCTGGG AAAACGTGCT 
GACCAACGCA AAGAGAAGCT TGGAGATTCT CATGACCTGC AGCGTTTCCT CAGTGATTTT 
AGAGACCTTA TGTCTTGGAT CAATGGAATC CGGGGTCTGG TCTCCTCAGA TGAACTTGCA 
AAAGATGTGA CTGGAGCTGA AGCTCTGTTG GAAAGGCATC AGGAGCACCG TACTGAAATA 
GATGCACGGG CTGGCACTTT TCAGGCATTT GAACAGTTTG GACAGCAGCT TCTGGCTCGT 
GGACACTATG CCAGCCCAGA GATTAAGGAG AAACTGGATA TTCTAGATCA AGAACGGACA 
GACCTAGAGA AGGCCTGGGT CCAGCGCAGA ATGATGCTAG ACCAGTGCTT AGAACTACAG 
CTGTTTCATC GGGATTGTGA ACAAGCTGAA AACTGGATGG CTGCCCGAGA GGCGTTCCTA 
AATACAGAAG ACAAAGGAGA CTCCTTAGAC AGCGTGGAGG CACTCATCAA GAAGCATGAA 
GATTTCGATA AAGCAATCAA TGTCCAGGAA GAGAAAATTG CTGTCTTGCA GTCTTTTGCT 
GACCAACTGA TTGCTGCTGA TCATTATGCA AAAGGAGTCA TTGCTAACAG ACGCAATGAG 
GTTCTGGACA GGTGGCGTCG TCTGAAGGCT CAGATGATTG AGAAGAGATC TAAGCTGGGA 
GAATCTCAGA CCCTCCAACA GTTCAGTCGT GATGTTGATG AAATAGAAGC TTGGATCAGT 
GAAAAGCTTC AAACTGCAAG TGATGAGTCA TATAAGGATC CCACAAACAT CCAGCTTTCC 
AAACTGCTGA GCAAGCACCA GAAGCACCAA GCCTTTGAAG CTGAGCTCCA CGCCAACGCA 
GATCGGATTC GTGGAGTCAT TGAAATGGGG AACTCTCTTA TTGAAAGAGG AGCGTGTGCT 
GGCAGCGAGG ATGCTGTGAA GGCTCGGCTG GCTGCCCTGG CTGACCAATG GGAGTTCCTG 
GTCCAGAAGT CATCAGAGAA GAGTCAGAAA CTGAAAGAAG CAAATAAACA GCAGAATTTC 
AATACCGGCA TCAAGGACTT TGATTTCTGG CTTTCAGAGG TGGAAGCTTT GTTGGCATCT 
GAAGACTATG GGAAGGACTT GGCATCAGTG AACAACCTTC TGAAGAAGCA CCAATTACTG 
GAAGCTGATA TATCTGCTCA TGAGGATCGG CTGAAGGACC TGAACAGCCA GGCTGACAGT 
TTGATGACCA GCAGTGCTTT CGATACCTCC CAAGTAAAGG ATAAACGTGA AACTATAAAT 
GGGCGCTTCC AGAGAATCAA GAGCATGGCA GCTGCCCGCC GTGCGAAGCT CAACGAGTCG 
CACCGCTTGC ATCAGTTCTT CCGTGACATG GATGATGAGG AGTCCTGGAT CAAAGAGAAG 
AAACTGTTGG TGAGCTCAGA GGACTATGGC AGAGACCTGA CTGGTGTGCA GAACCTGAGG 
AAGAAACATA AGCGCTTGGA AGCAGAATTA GCTGCCCATG AACCTGCTAT CCAGGGTGTT 
CTAGACACTG GCAAGAAGCT TTCAGATGAT AACACAATTG GGAAGGAGGA GATACAGCAA 
AGACTGGCTC AGTTTGTGGA CCACTGGAAA GAGTTAAAAC AGCTGGCAGC TGCTCGGGGG 
CAGCGTCTGG AGGAGTCCCT GGAGTACCAA CAGTTTGTAG CAAATGTTGA GGAAGAGGAG 
GCATGGATCA ACGAGAAAAT GACATTGGTA GCCAGTGAGG ATTATGGAGA CACACTTGCT 
GCTATCCAGG GCTTGCTGAA GAAGCATGAA GCATTCGAGA CTGACTTTAC TGTCCACAAA 
GACAGAGTGA ATGATGTTTG CGCTAATGGA GAGGATCTCA TTAAAAAGAA TAATCACCAT 
GTGGAGAACA TTACTGCTAA GATGAAGGGC CTCAAGGGCA AGGTATCAGA TCTGGAGAAA 
GCCGCAGCTC AGAGGAAAGC CAAACTGGAT GAGAACTCTG CCTTCCTCCA GTTCAACTGG 
AAAGCAGATG TGGTGGAGTC ATGGATAGGT GAGAAGGAAA ACAGTCTGAA GACAGATGAT 
TATGGCCGTG ACCTCTCCTC TGTGCAAACA CTACTCACCA AACAGGAAAC CTTTGATGCT 
GGACTTCAGG CTTTCCAGCA GGAGGGAATT GCAAACATCA CTGCTCTGAA AGACCAGCTA 
CTGGCAGCCA AACATATCCA ATCAAAGGCC ATTGAGGTTC GTCACGCTTC CTTGATGAAA 
CGCTGGAATC AGCTGCTTGC TAATTCTGCA GCCAGGAAAA AGAAACTCTT GGAGGCTCAG 
GAGCACTTCA GAAAGGTTGA AGATCTCTTC TTGACTTTTG CCAAGAAGGC CTCTGCCTTC 
AACAGTTGGT TTGAGAATGC TGAGGAGGAC CTGACGGATC CCGTGCGCTG CAATTCCCTG 
GAAGAAATCA AAGCACTGCG AGAAGCCCAC GATGCGTTCC GTTCCTCACT TAGCTCTGCC 
CAAGCTGACT TCAACCAGCT GGCAGAGCTT GATCGGCAGA TCAAGAGCTT CCGTGTAGCC 
TCCAACCCGT ACACTTGGTT TACTATGGAG GCTCTTGAAG AAACTTGGAG GAATCTGCAG 
AAAATTATCA AGGAGCGTGA ATTGGAGTTG CAGAAGGAGC AGCGGAGGCA GGAAGAAAAT 
GACAAGTTGC GCCAGGAGTT TGCTCAGCAT GCTAATGCCT TCCACCAGTG GATTCAGGAG 
ACAAGGACTT ACCTGCTAGA TGGCATAGCC TATCGTCGTG TCATTCGTGT CTATCAGTAT 
GAAGTTGGGG ATGATCTATC TGGAAGGTCC TGCATGGTGG AGGAGTCGGG AACACTGGAA 
TCCCAGCTGG AAGCTACTAA ACGCAAGCAC CAGGAGATCC GGGCTATGAG GAGCCAGCTG 
AAGAAGATTG AGGACCTTGG AGCAGCCATG GAAGAGGCAC TTATCTTGGA CAACAAATAC 
ACAGAGCACA GCACCGTGGG GCTGGCGCAG CAGTGGGACC AGCTTGACCA GCTGGGAATG 
AGAATGCAGC ACAATCTGGA ACAGCAGATT CAAGCTCGAA ACACAACTGG AGTCACAGAG 
GAGGCCCTGA AGGAGTTCAG CATGATGTTC AAGCACTTTG ACAAGGACAA ATCTGGACGT 
CTTAATCACC AGGAGTTTAA GTCTTGCTTG CGCTCCCTTG GCTATGACCT GCCCATGGTT 
GAGGAAGGAG AGCCTGACCC CGAATTTGAG TCTATTCTTG ACACTGTTGA TCCCAACAGG 
GATGGCCACG TCTCGCTGCA GGAGTACATG GCGTTCATGA TCAGCAGGGA AACAGAGAAC 
GTGAAATCCA GCGAGGAGAT TGAGAGTGCT TTCCGTGCCC TCAGCTCGGA GGGGAAGCCT 
TACGTGACCA AGGAGGAGCT CTACCAGAAC CTGACCCGGG AGCAGGCCGA CTACTGCATT 
TCTCACATGA AGCCCTACAT GGATGGCAAG GGCAGAGAAC TTCCTTCTGC CTACGACTAC 
ATAGAATTTA CACGTTCACT CTTTGTGAAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_015279287.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015134773.1 Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X6, mRNA.

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CloneID	OGa43313
Clone ID Related Accession (Same CDS sequence)	XM_015279287.1 , XM_015279287.2
Accession Version	XM_015279287.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7533bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015279287.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG 
TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC 
TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT 
GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT 
GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG 
ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAA CTCGTCTACA GGAGCTGCAC 
CGACTATGGG AGTTACTGCT GGAAAAAATG AGAGAGAAGG GAGTGAAGCT GTTGCAAGCA 
CAGAAGCTGG TGCAATTCTT ACGGGAATGT GAAGATGTCA TGGACTGGAT CAATGACAAG 
GAAGCAATAG TGACCTCAGA AGAGCTTGGA CAGGACTTAG AGCATGTTGA GGTTTTGCAG 
AAGAAATTTG AAGAGTTCCA AACAGATCTG GCAGCTCATG AGGAGAGAGT GAATGAAGTG 
AACCAGTTCG CTGGCAAACT TATCCAGGAA CAGCACCCTG AGGAGGAACT TATAAAGTCC 
AAACAGGATG AAGTAAATGC AAGCTGGCAG CGTCTTAAGG GGCTTGCCCA ACAGAGGCAA 
GGAAAACTCT TTGGGGCAGC TGAAGTTCAG CGCTTCAACA GGGATGTGGA TGAAACTATC 
AGCTGGATTA AGGAGAAAGG GCAGTTGATG GCCTCAGATG ACTTTGGCAG AGACTTGGCC 
AGCGTGCAAG CTTTACTACG TAAGCATGAA GGCCTGGAAA GAGATCTTGC AGCTTTGGAG 
GATAAGGTTA AGGCCCTCTG TGCAGAAGCT GACCGTTTGC AGCAGTCTCA CCCTATAAAT 
GCTTCTCAAA TTCAAGTGAA ACGGGAGGAA CTGATTGCCA ACTGGGAGCA GATCCGAACT 
CTGGCAGCAG AGAGGCATGC TCGCCTTAAT GACTCCTACA GGTTGCAGCG CTTTCTTGCA 
GATTTCCGGG ACCTCACTAG CTGGGTAACT GAGATGAAGG CTCTGATAAA TGCTGATGAG 
CTTGCCAATG ATGTGGCTGG AGCAGAAGCC CTTCTAGATA GACATCAGGA ACATAAGGGA 
GAAATTGATG CTCATGAGGA TAGCTTCAGA TCTGCTGATG AGTCTGGGCA GGCTTTGCTT 
GCAGCTGGGC ACTATGCTTC TGATGAAGTT AAAGAAAAGC TGACTATCCT CTCAGATGAA 
AGATCAGCCT TGCTGGAACT ATGGGAGCTT CGCAGGCAAC AGTATGAGCA GTGCATGGAT 
CTGCAGCTTT TCTACAGAGA TACTGAACAA GTTGACAACT GGATGAGCAA ACAAGAAGCT 
TTTCTGCTGA ACGAAGACCT TGGTGATTCT CTGGATAGCG TGGAGGCTCT TCTAAAGAAG 
CATGAAGATT TTGAGAAATC CCTAAGTGCT CAAGAGGAGA AGATCACAGC ACTGGATGAG 
TTTGCTACTA AACTGATTCA AAATAACCAT TATGCCATGG ATGACGTTGC TACGCGCAGA 
GATGCTCTTC TGAGTCGCCG AAATGCTCTT CATGAAAGAG CCATGAAGCG CCGTGCCCAA 
CTGGCAGACT CTTTCCATCT CCAGCAGTTT TTCAGGGACT CTGATGAACT GAAGAGTTGG 
GTTAATGAAA AAATGAAAAC TGCTACCGAT GAGGCTTACA AGGATCCATC CAACTTGCAA 
GGTAAAGTTC AGAAGCATCA GGCTTTTGAA GCAGAGCTTT CTGCTAATCA GAGTCGTATT 
GATGCACTGG AGAAAGCTGG CCAGAAGTTG ATTGATGTCA ATCACTATGC ATCTGATGAG 
GTGGCAGCTC GCATGAATGA AGTCATCAGC TTGTGGAAGA AACTTCTGGA AGCCACCGAG 
CTCAAAGGTA TAAAGCTGCG TGAAGCCAAT CAACAGCAGC AATTTAATCG CAATGTAGAA 
GACATTGAGT TGTGGCTGTA TGAAGTGGAG GGTCACTTGG CTTCTGATGA TTATGGAAAA 
GATCTTACCA GCGTTCAGAA TCTTCAGAAG AAACATGCCC TGCTAGAGGC AGATGTTGCT 
GCCCATCAGG ATCGGATAGA TGGCATTACC ATCCAGGCAC GCCAGTTCCA AGATGCTGGG 
CACTTTGATG CTGACAATAT CAAGAAGAAA CAAGAAGCTT TAGTAGCTCG TTATGAAGCT 
CTGAAGGATC CTATGGTGGC TCGCAAGCAG AAACTTGCAG ATTCTCTTCG CCTGCAGCAG 
CTTTTCCGTG ACATCGAGGA TGAAGAGACC TGGATCAGGG AAAAGGAACC TATTGCAGCC 
TCAACAAACC GAGGCAAGGA CTTAATTGGT GTCCAGAATC TGCTAAAGAA GCACCAGGCT 
TTGCAGGCAG AAATTGCAGG CCATGAGCCT CGCATTAAAG CAGTCACACA AAAAGGAAAT 
GCGATGGTGG AAGAGGGACA TTTTGCTGCT GAAGATGTGA AGATCAAATT GAATGAACTA 
AACCAAAAAT GGGACTCTCT GAAAGCCAAG GCATCTCAAC GGCGACAGGA TCTAGAGGAT 
TCTCTGCAAG CTCAGCAGTA TTTTGCTGAT GCTAATGAGG CAGAATCATG GATGAGGGAA 
AAGGAGCCCA TTGTAGGCAG CACGGATTAT GGAAAGGATG AAGACTCTGC TGAGGCTCTC 
CTGAAGAAGC ATGAAGCTTT GATGTCTGAT CTTTCTGCTT ACGGCAGTAG CATACAGGCA 
TTAAGGGAAC AGGCCCAGTC CTGCAGGCAA CAAGTTGCTC CCACTGATGA TGAAACTGGA 
AAAGAGCTTG TGCTAGCACT CTATGATTAC CAAGAGAAGA GTCCTCGGGA GGTGACTATG 
AAGAAAGGAG ATATTCTAAC CCTGCTCAAC AGCACCAACA AGGACTGGTG GAAGGTTGAA 
GTTAACGATC GTCAGGGCTT TGTACCAGCT GCCTATGTGA AAAAACTAGA TCCTGCCCAG 
TCTGCATCCC GAGAGAATCT TCTAGAAGAG CAAGGCAGCA TAGCATTGCG ACAGGAGCAA 
ATTGACAACC AGACTCTCAT TACTAAGGAA GTCGGCAGTG TATCTCTGCG TATGAAACAG 
GTCGAAGAAC TGTATCATTC CCTTCTTGAG CTGGGAGAAA AACGTAAAGG CATGCTAGAA 
AAAAGCTGCA AGAAGTTTAT GCTTTTCCGT GAGGCTAATG AGCTTCAACA GTGGATCAAT 
GAGAAGGAAG CTGCGCTCAC TAATGAGGAA GTGGGTGCTG ATTTGGAGCA GGTGGAGGTG 
CTACAGAAGA AGTTTGATGA TTTTCAGAAG GATTTAAAAG CTAATGAGTC ACGACTGAAG 
GACATAAACA AGGTTGCAAA TGACCTGGAG TCTGAAGGGC TGATGGCAGA GGAAGTGCAA 
GCAGTAGAGC ACCAGGAAGT CTATGGAATG ATGCCCAGAG ATGAAACTGA TTCTAAGACA 
GCCTCTCCTT GGAAGTCTGC ACGTATGATG GTACACACAG TGGCAACGTT TAACTCAATC 
AAGGAACTGA ATGAACGCTG GAGATCCCTG CAGCAGTTGG CAGAGGAGCG AAGCCAGCTG 
TTGGGCAGCG CTCATGAAGT CCAGAGGTTC CACAGAGATG CTGATGAAAC AAAAGAATGG 
ATAGAAGAGA AGAATCAAGC ATTAAATACC GACAACTATG GACATGACTT GGCCAGTGTG 
CAGGCTCTGC AGCGCAAGCA TGAAGGCTTT GAGAGAGACT TGGCAGCTCT TGGAGACAAG 
GTGAATTCTC TTGGTGAAAC TGCCCAGCGT CTGATCCAGT CACATCCAGA ATCAGCTGAA 
GATCTTCAAG AAAAATGTAC TGAGTTGAAC CAAGCCTGGA GTAGTCTGGG AAAACGTGCT 
GACCAACGCA AAGAGAAGCT TGGAGATTCT CATGACCTGC AGCGTTTCCT CAGTGATTTT 
AGAGACCTTA TGTCTTGGAT CAATGGAATC CGGGGTCTGG TCTCCTCAGA TGAACTTGCA 
AAAGATGTGA CTGGAGCTGA AGCTCTGTTG GAAAGGCATC AGGAGCACCG TACTGAAATA 
GATGCACGGG CTGGCACTTT TCAGGCATTT GAACAGTTTG GACAGCAGCT TCTGGCTCGT 
GGACACTATG CCAGCCCAGA GATTAAGGAG AAACTGGATA TTCTAGATCA AGAACGGACA 
GACCTAGAGA AGGCCTGGGT CCAGCGCAGA ATGATGCTAG ACCAGTGCTT AGAACTACAG 
CTGTTTCATC GGGATTGTGA ACAAGCTGAA AACTGGATGG CTGCCCGAGA GGCGTTCCTA 
AATACAGAAG ACAAAGGAGA CTCCTTAGAC AGCGTGGAGG CACTCATCAA GAAGCATGAA 
GATTTCGATA AAGCAATCAA TGTCCAGGAA GAGAAAATTG CTGTCTTGCA GTCTTTTGCT 
GACCAACTGA TTGCTGCTGA TCATTATGCA AAAGGAGTCA TTGCTAACAG ACGCAATGAG 
GTTCTGGACA GGTGGCGTCG TCTGAAGGCT CAGATGATTG AGAAGAGATC TAAGCTGGGA 
GAATCTCAGA CCCTCCAACA GTTCAGTCGT GATGTTGATG AAATAGAAGC TTGGATCAGT 
GAAAAGCTTC AAACTGCAAG TGATGAGTCA TATAAGGATC CCACAAACAT CCAGCTTTCC 
AAACTGCTGA GCAAGCACCA GAAGCACCAA GCCTTTGAAG CTGAGCTCCA CGCCAACGCA 
GATCGGATTC GTGGAGTCAT TGAAATGGGG AACTCTCTTA TTGAAAGAGG AGCGTGTGCT 
GGCAGCGAGG ATGCTGTGAA GGCTCGGCTG GCTGCCCTGG CTGACCAATG GGAGTTCCTG 
GTCCAGAAGT CATCAGAGAA GAGTCAGAAA CTGAAAGAAG CAAATAAACA GCAGAATTTC 
AATACCGGCA TCAAGGACTT TGATTTCTGG CTTTCAGAGG TGGAAGCTTT GTTGGCATCT 
GAAGACTATG GGAAGGACTT GGCATCAGTG AACAACCTTC TGAAGAAGCA CCAATTACTG 
GAAGCTGATA TATCTGCTCA TGAGGATCGG CTGAAGGACC TGAACAGCCA GGCTGACAGT 
TTGATGACCA GCAGTGCTTT CGATACCTCC CAAGTAAAGG ATAAACGTGA AACTATAAAT 
GGGCGCTTCC AGAGAATCAA GAGCATGGCA GCTGCCCGCC GTGCGAAGCT CAACGAGTCG 
CACCGCTTGC ATCAGTTCTT CCGTGACATG GATGATGAGG AGTCCTGGAT CAAAGAGAAG 
AAACTGTTGG TGAGCTCAGA GGACTATGGC AGAGACCTGA CTGGTGTGCA GAACCTGAGG 
AAGAAACATA AGCGCTTGGA AGCAGAATTA GCTGCCCATG AACCTGCTAT CCAGGGTGTT 
CTAGACACTG GCAAGAAGCT TTCAGATGAT AACACAATTG GGAAGGAGGA GATACAGCAA 
AGACTGGCTC AGTTTGTGGA CCACTGGAAA GAGTTAAAAC AGCTGGCAGC TGCTCGGGGG 
CAGCGTCTGG AGGAGTCCCT GGAGTACCAA CAGTTTGTAG CAAATGTTGA GGAAGAGGAG 
GCATGGATCA ACGAGAAAAT GACATTGGTA GCCAGTGAGG ATTATGGAGA CACACTTGCT 
GCTATCCAGG GCTTGCTGAA GAAGCATGAA GCATTCGAGA CTGACTTTAC TGTCCACAAA 
GACAGAGTGA ATGATGTTTG CGCTAATGGA GAGGATCTCA TTAAAAAGAA TAATCACCAT 
GTGGAGAACA TTACTGCTAA GATGAAGGGC CTCAAGGGCA AGGTATCAGA TCTGGAGAAA 
GCCGCAGCTC AGAGGAAAGC CAAACTGGAT GAGAACTCTG CCTTCCTCCA GTTCAACTGG 
AAAGCAGATG TGGTGGAGTC ATGGATAGGT GAGAAGGAAA ACAGTCTGAA GACAGATGAT 
TATGGCCGTG ACCTCTCCTC TGTGCAAACA CTACTCACCA AACAGGAAAC CTTTGATGCT 
GGACTTCAGG CTTTCCAGCA GGAGGGAATT GCAAACATCA CTGCTCTGAA AGACCAGCTA 
CTGGCAGCCA AACATATCCA ATCAAAGGCC ATTGAGGTTC GTCACGCTTC CTTGATGAAA 
CGCTGGAATC AGCTGCTTGC TAATTCTGCA GCCAGGAAAA AGAAACTCTT GGAGGCTCAG 
GAGCACTTCA GAAAGGTTGA AGATCTCTTC TTGACTTTTG CCAAGAAGGC CTCTGCCTTC 
AACAGTTGGT TTGAGAATGC TGAGGAGGAC CTGACGGATC CCGTGCGCTG CAATTCCCTG 
GAAGAAATCA AAGCACTGCG AGAAGCCCAC GATGCGTTCC GTTCCTCACT TAGCTCTGCC 
CAAGCTGACT TCAACCAGCT GGCAGAGCTT GATCGGCAGA TCAAGAGCTT CCGTGTAGCC 
TCCAACCCGT ACACTTGGTT TACTATGGAG GCTCTTGAAG AAACTTGGAG GAATCTGCAG 
AAAATTATCA AGGAGCGTGA ATTGGAGTTG CAGAAGGAGC AGCGGAGGCA GGAAGAAAAT 
GACAAGTTGC GCCAGGAGTT TGCTCAGCAT GCTAATGCCT TCCACCAGTG GATTCAGGAG 
ACAAGGACTT ACCTGCTAGA TGGCATAGCC TATCGTCGTG TCATTCGTGT CTATCAGTAT 
GAAGTTGGGG ATGATCTATC TGGAAGGTCC TGCATGGTGG AGGAGTCGGG AACACTGGAA 
TCCCAGCTGG AAGCTACTAA ACGCAAGCAC CAGGAGATCC GGGCTATGAG GAGCCAGCTG 
AAGAAGATTG AGGACCTTGG AGCAGCCATG GAAGAGGCAC TTATCTTGGA CAACAAATAC 
ACAGAGCACA GCACCGTGGG GCTGGCGCAG CAGTGGGACC AGCTTGACCA GCTGGGAATG 
AGAATGCAGC ACAATCTGGA ACAGCAGATT CAAGCTCGAA ACACAACTGG AGTCACAGAG 
GAGGCCCTGA AGGAGTTCAG CATGATGTTC AAGCACTTTG ACAAGGACAA ATCTGGACGT 
CTTAATCACC AGGAGTTTAA GTCTTGCTTG CGCTCCCTTG GCTATGACCT GCCCATGGTT 
GAGGAAGGAG AGCCTGACCC CGAATTTGAG TCTATTCTTG ACACTGTTGA TCCCAACAGG 
GATGGCCACG TCTCGCTGCA GGAGTACATG GCGTTCATGA TCAGCAGGGA AACAGAGAAC 
GTGAAATCCA GCGAGGAGAT TGAGAGTGCT TTCCGTGCCC TCAGCTCGGA GGGGAAGCCT 
TACGTGACCA AGGAGGAGCT CTACCAGAAC CTGACCCGGG AGCAGGCCGA CTACTGCATT 
TCTCACATGA AGCCCTACAT GGATGGCAAG GGCAGAGAAC TTCCTTCTGC CTACGACTAC 
ATAGAATTTA CACGTTCACT CTTTGTGAAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015134773.1
CDS	15..7547
Translation	MLSSFRKVKVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSSLRRQKLEDSYRFQFFQRDADELEKWIQEKLQIASDENYKDPSNLQGKLQKHQAFEAEVQANSGAIVKLDETGNQMINEGHFASETIRTRLQELHRLWELLLEKMREKGVKLLQAQKLVQFLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDLAAHEERVNEVNQFAGKLIQEQHPEEELIKSKQDEVNASWQRLKGLAQQRQGKLFGAAEVQRFNRDVDETISWIKEKGQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPINASQIQVKREELIANWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFRSADESGQALLAAGHYASDEVKEKLTILSDERSALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMDDVATRRDALLSRRNALHERAMKRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYASDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTSVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDADNIKKKQEALVARYEALKDPMVARKQKLADSLRLQQLFRDIEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKIKLNELNQKWDSLKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPTDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTLITKEVGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTNEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVANDLESEGLMAEEVQAVEHQEVYGMMPRDETDSKTASPWKSARMMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAQRLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKEKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLARGHYASPEIKEKLDILDQERTDLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAVLQSFADQLIAADHYAKGVIANRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIEMGNSLIERGACAGSEDAVKARLAALADQWEFLVQKSSEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRETINGRFQRIKSMAAARRAKLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVDHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCANGEDLIKKNNHHVENITAKMKGLKGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHIQSKAIEVRHASLMKRWNQLLANSAARKKKLLEAQEHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGIAYRRVIRVYQYEVGDDLSGRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFESILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCISHMKPYMDGKGRELPSAYDYIEFTRSLFVN

Target ORF information:

RefSeq Version	XM_015279287.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279287.2

ORF Insert Sequence:

ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG 
TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC 
TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT 
GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT 
GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG 
ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAA CTCGTCTACA GGAGCTGCAC 
CGACTATGGG AGTTACTGCT GGAAAAAATG AGAGAGAAGG GAGTGAAGCT GTTGCAAGCA 
CAGAAGCTGG TGCAATTCTT ACGGGAATGT GAAGATGTCA TGGACTGGAT CAATGACAAG 
GAAGCAATAG TGACCTCAGA AGAGCTTGGA CAGGACTTAG AGCATGTTGA GGTTTTGCAG 
AAGAAATTTG AAGAGTTCCA AACAGATCTG GCAGCTCATG AGGAGAGAGT GAATGAAGTG 
AACCAGTTCG CTGGCAAACT TATCCAGGAA CAGCACCCTG AGGAGGAACT TATAAAGTCC 
AAACAGGATG AAGTAAATGC AAGCTGGCAG CGTCTTAAGG GGCTTGCCCA ACAGAGGCAA 
GGAAAACTCT TTGGGGCAGC TGAAGTTCAG CGCTTCAACA GGGATGTGGA TGAAACTATC 
AGCTGGATTA AGGAGAAAGG GCAGTTGATG GCCTCAGATG ACTTTGGCAG AGACTTGGCC 
AGCGTGCAAG CTTTACTACG TAAGCATGAA GGCCTGGAAA GAGATCTTGC AGCTTTGGAG 
GATAAGGTTA AGGCCCTCTG TGCAGAAGCT GACCGTTTGC AGCAGTCTCA CCCTATAAAT 
GCTTCTCAAA TTCAAGTGAA ACGGGAGGAA CTGATTGCCA ACTGGGAGCA GATCCGAACT 
CTGGCAGCAG AGAGGCATGC TCGCCTTAAT GACTCCTACA GGTTGCAGCG CTTTCTTGCA 
GATTTCCGGG ACCTCACTAG CTGGGTAACT GAGATGAAGG CTCTGATAAA TGCTGATGAG 
CTTGCCAATG ATGTGGCTGG AGCAGAAGCC CTTCTAGATA GACATCAGGA ACATAAGGGA 
GAAATTGATG CTCATGAGGA TAGCTTCAGA TCTGCTGATG AGTCTGGGCA GGCTTTGCTT 
GCAGCTGGGC ACTATGCTTC TGATGAAGTT AAAGAAAAGC TGACTATCCT CTCAGATGAA 
AGATCAGCCT TGCTGGAACT ATGGGAGCTT CGCAGGCAAC AGTATGAGCA GTGCATGGAT 
CTGCAGCTTT TCTACAGAGA TACTGAACAA GTTGACAACT GGATGAGCAA ACAAGAAGCT 
TTTCTGCTGA ACGAAGACCT TGGTGATTCT CTGGATAGCG TGGAGGCTCT TCTAAAGAAG 
CATGAAGATT TTGAGAAATC CCTAAGTGCT CAAGAGGAGA AGATCACAGC ACTGGATGAG 
TTTGCTACTA AACTGATTCA AAATAACCAT TATGCCATGG ATGACGTTGC TACGCGCAGA 
GATGCTCTTC TGAGTCGCCG AAATGCTCTT CATGAAAGAG CCATGAAGCG CCGTGCCCAA 
CTGGCAGACT CTTTCCATCT CCAGCAGTTT TTCAGGGACT CTGATGAACT GAAGAGTTGG 
GTTAATGAAA AAATGAAAAC TGCTACCGAT GAGGCTTACA AGGATCCATC CAACTTGCAA 
GGTAAAGTTC AGAAGCATCA GGCTTTTGAA GCAGAGCTTT CTGCTAATCA GAGTCGTATT 
GATGCACTGG AGAAAGCTGG CCAGAAGTTG ATTGATGTCA ATCACTATGC ATCTGATGAG 
GTGGCAGCTC GCATGAATGA AGTCATCAGC TTGTGGAAGA AACTTCTGGA AGCCACCGAG 
CTCAAAGGTA TAAAGCTGCG TGAAGCCAAT CAACAGCAGC AATTTAATCG CAATGTAGAA 
GACATTGAGT TGTGGCTGTA TGAAGTGGAG GGTCACTTGG CTTCTGATGA TTATGGAAAA 
GATCTTACCA GCGTTCAGAA TCTTCAGAAG AAACATGCCC TGCTAGAGGC AGATGTTGCT 
GCCCATCAGG ATCGGATAGA TGGCATTACC ATCCAGGCAC GCCAGTTCCA AGATGCTGGG 
CACTTTGATG CTGACAATAT CAAGAAGAAA CAAGAAGCTT TAGTAGCTCG TTATGAAGCT 
CTGAAGGATC CTATGGTGGC TCGCAAGCAG AAACTTGCAG ATTCTCTTCG CCTGCAGCAG 
CTTTTCCGTG ACATCGAGGA TGAAGAGACC TGGATCAGGG AAAAGGAACC TATTGCAGCC 
TCAACAAACC GAGGCAAGGA CTTAATTGGT GTCCAGAATC TGCTAAAGAA GCACCAGGCT 
TTGCAGGCAG AAATTGCAGG CCATGAGCCT CGCATTAAAG CAGTCACACA AAAAGGAAAT 
GCGATGGTGG AAGAGGGACA TTTTGCTGCT GAAGATGTGA AGATCAAATT GAATGAACTA 
AACCAAAAAT GGGACTCTCT GAAAGCCAAG GCATCTCAAC GGCGACAGGA TCTAGAGGAT 
TCTCTGCAAG CTCAGCAGTA TTTTGCTGAT GCTAATGAGG CAGAATCATG GATGAGGGAA 
AAGGAGCCCA TTGTAGGCAG CACGGATTAT GGAAAGGATG AAGACTCTGC TGAGGCTCTC 
CTGAAGAAGC ATGAAGCTTT GATGTCTGAT CTTTCTGCTT ACGGCAGTAG CATACAGGCA 
TTAAGGGAAC AGGCCCAGTC CTGCAGGCAA CAAGTTGCTC CCACTGATGA TGAAACTGGA 
AAAGAGCTTG TGCTAGCACT CTATGATTAC CAAGAGAAGA GTCCTCGGGA GGTGACTATG 
AAGAAAGGAG ATATTCTAAC CCTGCTCAAC AGCACCAACA AGGACTGGTG GAAGGTTGAA 
GTTAACGATC GTCAGGGCTT TGTACCAGCT GCCTATGTGA AAAAACTAGA TCCTGCCCAG 
TCTGCATCCC GAGAGAATCT TCTAGAAGAG CAAGGCAGCA TAGCATTGCG ACAGGAGCAA 
ATTGACAACC AGACTCTCAT TACTAAGGAA GTCGGCAGTG TATCTCTGCG TATGAAACAG 
GTCGAAGAAC TGTATCATTC CCTTCTTGAG CTGGGAGAAA AACGTAAAGG CATGCTAGAA 
AAAAGCTGCA AGAAGTTTAT GCTTTTCCGT GAGGCTAATG AGCTTCAACA GTGGATCAAT 
GAGAAGGAAG CTGCGCTCAC TAATGAGGAA GTGGGTGCTG ATTTGGAGCA GGTGGAGGTG 
CTACAGAAGA AGTTTGATGA TTTTCAGAAG GATTTAAAAG CTAATGAGTC ACGACTGAAG 
GACATAAACA AGGTTGCAAA TGACCTGGAG TCTGAAGGGC TGATGGCAGA GGAAGTGCAA 
GCAGTAGAGC ACCAGGAAGT CTATGGAATG ATGCCCAGAG ATGAAACTGA TTCTAAGACA 
GCCTCTCCTT GGAAGTCTGC ACGTATGATG GTACACACAG TGGCAACGTT TAACTCAATC 
AAGGAACTGA ATGAACGCTG GAGATCCCTG CAGCAGTTGG CAGAGGAGCG AAGCCAGCTG 
TTGGGCAGCG CTCATGAAGT CCAGAGGTTC CACAGAGATG CTGATGAAAC AAAAGAATGG 
ATAGAAGAGA AGAATCAAGC ATTAAATACC GACAACTATG GACATGACTT GGCCAGTGTG 
CAGGCTCTGC AGCGCAAGCA TGAAGGCTTT GAGAGAGACT TGGCAGCTCT TGGAGACAAG 
GTGAATTCTC TTGGTGAAAC TGCCCAGCGT CTGATCCAGT CACATCCAGA ATCAGCTGAA 
GATCTTCAAG AAAAATGTAC TGAGTTGAAC CAAGCCTGGA GTAGTCTGGG AAAACGTGCT 
GACCAACGCA AAGAGAAGCT TGGAGATTCT CATGACCTGC AGCGTTTCCT CAGTGATTTT 
AGAGACCTTA TGTCTTGGAT CAATGGAATC CGGGGTCTGG TCTCCTCAGA TGAACTTGCA 
AAAGATGTGA CTGGAGCTGA AGCTCTGTTG GAAAGGCATC AGGAGCACCG TACTGAAATA 
GATGCACGGG CTGGCACTTT TCAGGCATTT GAACAGTTTG GACAGCAGCT TCTGGCTCGT 
GGACACTATG CCAGCCCAGA GATTAAGGAG AAACTGGATA TTCTAGATCA AGAACGGACA 
GACCTAGAGA AGGCCTGGGT CCAGCGCAGA ATGATGCTAG ACCAGTGCTT AGAACTACAG 
CTGTTTCATC GGGATTGTGA ACAAGCTGAA AACTGGATGG CTGCCCGAGA GGCGTTCCTA 
AATACAGAAG ACAAAGGAGA CTCCTTAGAC AGCGTGGAGG CACTCATCAA GAAGCATGAA 
GATTTCGATA AAGCAATCAA TGTCCAGGAA GAGAAAATTG CTGTCTTGCA GTCTTTTGCT 
GACCAACTGA TTGCTGCTGA TCATTATGCA AAAGGAGTCA TTGCTAACAG ACGCAATGAG 
GTTCTGGACA GGTGGCGTCG TCTGAAGGCT CAGATGATTG AGAAGAGATC TAAGCTGGGA 
GAATCTCAGA CCCTCCAACA GTTCAGTCGT GATGTTGATG AAATAGAAGC TTGGATCAGT 
GAAAAGCTTC AAACTGCAAG TGATGAGTCA TATAAGGATC CCACAAACAT CCAGCTTTCC 
AAACTGCTGA GCAAGCACCA GAAGCACCAA GCCTTTGAAG CTGAGCTCCA CGCCAACGCA 
GATCGGATTC GTGGAGTCAT TGAAATGGGG AACTCTCTTA TTGAAAGAGG AGCGTGTGCT 
GGCAGCGAGG ATGCTGTGAA GGCTCGGCTG GCTGCCCTGG CTGACCAATG GGAGTTCCTG 
GTCCAGAAGT CATCAGAGAA GAGTCAGAAA CTGAAAGAAG CAAATAAACA GCAGAATTTC 
AATACCGGCA TCAAGGACTT TGATTTCTGG CTTTCAGAGG TGGAAGCTTT GTTGGCATCT 
GAAGACTATG GGAAGGACTT GGCATCAGTG AACAACCTTC TGAAGAAGCA CCAATTACTG 
GAAGCTGATA TATCTGCTCA TGAGGATCGG CTGAAGGACC TGAACAGCCA GGCTGACAGT 
TTGATGACCA GCAGTGCTTT CGATACCTCC CAAGTAAAGG ATAAACGTGA AACTATAAAT 
GGGCGCTTCC AGAGAATCAA GAGCATGGCA GCTGCCCGCC GTGCGAAGCT CAACGAGTCG 
CACCGCTTGC ATCAGTTCTT CCGTGACATG GATGATGAGG AGTCCTGGAT CAAAGAGAAG 
AAACTGTTGG TGAGCTCAGA GGACTATGGC AGAGACCTGA CTGGTGTGCA GAACCTGAGG 
AAGAAACATA AGCGCTTGGA AGCAGAATTA GCTGCCCATG AACCTGCTAT CCAGGGTGTT 
CTAGACACTG GCAAGAAGCT TTCAGATGAT AACACAATTG GGAAGGAGGA GATACAGCAA 
AGACTGGCTC AGTTTGTGGA CCACTGGAAA GAGTTAAAAC AGCTGGCAGC TGCTCGGGGG 
CAGCGTCTGG AGGAGTCCCT GGAGTACCAA CAGTTTGTAG CAAATGTTGA GGAAGAGGAG 
GCATGGATCA ACGAGAAAAT GACATTGGTA GCCAGTGAGG ATTATGGAGA CACACTTGCT 
GCTATCCAGG GCTTGCTGAA GAAGCATGAA GCATTCGAGA CTGACTTTAC TGTCCACAAA 
GACAGAGTGA ATGATGTTTG CGCTAATGGA GAGGATCTCA TTAAAAAGAA TAATCACCAT 
GTGGAGAACA TTACTGCTAA GATGAAGGGC CTCAAGGGCA AGGTATCAGA TCTGGAGAAA 
GCCGCAGCTC AGAGGAAAGC CAAACTGGAT GAGAACTCTG CCTTCCTCCA GTTCAACTGG 
AAAGCAGATG TGGTGGAGTC ATGGATAGGT GAGAAGGAAA ACAGTCTGAA GACAGATGAT 
TATGGCCGTG ACCTCTCCTC TGTGCAAACA CTACTCACCA AACAGGAAAC CTTTGATGCT 
GGACTTCAGG CTTTCCAGCA GGAGGGAATT GCAAACATCA CTGCTCTGAA AGACCAGCTA 
CTGGCAGCCA AACATATCCA ATCAAAGGCC ATTGAGGTTC GTCACGCTTC CTTGATGAAA 
CGCTGGAATC AGCTGCTTGC TAATTCTGCA GCCAGGAAAA AGAAACTCTT GGAGGCTCAG 
GAGCACTTCA GAAAGGTTGA AGATCTCTTC TTGACTTTTG CCAAGAAGGC CTCTGCCTTC 
AACAGTTGGT TTGAGAATGC TGAGGAGGAC CTGACGGATC CCGTGCGCTG CAATTCCCTG 
GAAGAAATCA AAGCACTGCG AGAAGCCCAC GATGCGTTCC GTTCCTCACT TAGCTCTGCC 
CAAGCTGACT TCAACCAGCT GGCAGAGCTT GATCGGCAGA TCAAGAGCTT CCGTGTAGCC 
TCCAACCCGT ACACTTGGTT TACTATGGAG GCTCTTGAAG AAACTTGGAG GAATCTGCAG 
AAAATTATCA AGGAGCGTGA ATTGGAGTTG CAGAAGGAGC AGCGGAGGCA GGAAGAAAAT 
GACAAGTTGC GCCAGGAGTT TGCTCAGCAT GCTAATGCCT TCCACCAGTG GATTCAGGAG 
ACAAGGACTT ACCTGCTAGA TGGCATAGCC TATCGTCGTG TCATTCGTGT CTATCAGTAT 
GAAGTTGGGG ATGATCTATC TGGAAGGTCC TGCATGGTGG AGGAGTCGGG AACACTGGAA 
TCCCAGCTGG AAGCTACTAA ACGCAAGCAC CAGGAGATCC GGGCTATGAG GAGCCAGCTG 
AAGAAGATTG AGGACCTTGG AGCAGCCATG GAAGAGGCAC TTATCTTGGA CAACAAATAC 
ACAGAGCACA GCACCGTGGG GCTGGCGCAG CAGTGGGACC AGCTTGACCA GCTGGGAATG 
AGAATGCAGC ACAATCTGGA ACAGCAGATT CAAGCTCGAA ACACAACTGG AGTCACAGAG 
GAGGCCCTGA AGGAGTTCAG CATGATGTTC AAGCACTTTG ACAAGGACAA ATCTGGACGT 
CTTAATCACC AGGAGTTTAA GTCTTGCTTG CGCTCCCTTG GCTATGACCT GCCCATGGTT 
GAGGAAGGAG AGCCTGACCC CGAATTTGAG TCTATTCTTG ACACTGTTGA TCCCAACAGG 
GATGGCCACG TCTCGCTGCA GGAGTACATG GCGTTCATGA TCAGCAGGGA AACAGAGAAC 
GTGAAATCCA GCGAGGAGAT TGAGAGTGCT TTCCGTGCCC TCAGCTCGGA GGGGAAGCCT 
TACGTGACCA AGGAGGAGCT CTACCAGAAC CTGACCCGGG AGCAGGCCGA CTACTGCATT 
TCTCACATGA AGCCCTACAT GGATGGCAAG GGCAGAGAAC TTCCTTCTGC CTACGACTAC 
ATAGAATTTA CACGTTCACT CTTTGTGAAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_015279288.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015134774.1 Gallus gallus spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X7, mRNA.

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CloneID	OGa43314
Clone ID Related Accession (Same CDS sequence)	XM_015279288.1 , XM_015279288.2
Accession Version	XM_015279288.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7521bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG 
TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC 
TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT 
GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT 
GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG 
ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAT CTTTCACGCC TTACAGGACT 
GCACAGCGCC CAATAGTAAC AGCCACCCAA GTTGGGTCCC ACATACGAAC TCGTCTACAG 
GAGCTGCACC GACTATGGGA GTTACTGCTG GAAAAAATGA GAGAGAAGGG AGTGAAGCTG 
TTGCAAGCAC AGAAGCTGGT GCAATTCTTA CGGGAATGTG AAGATGTCAT GGACTGGATC 
AATGACAAGG AAGCAATAGT GACCTCAGAA GAGCTTGGAC AGGACTTAGA GCATGTTGAG 
GTTTTGCAGA AGAAATTTGA AGAGTTCCAA ACAGATCTGG CAGCTCATGA GGAGAGAGTG 
AATGAAGTGA ACCAGTTCGC TGGCAAACTT ATCCAGGAAC AGCACCCTGA GGAGGAACTT 
ATAAAGTCCA AACAGGATGA AGTAAATGCA AGCTGGCAGC GTCTTAAGGG GCTTGCCCAA 
CAGAGGCAAG GAAAACTCTT TGGGGCAGCT GAAGTTCAGC GCTTCAACAG GGATGTGGAT 
GAAACTATCA GCTGGATTAA GGAGAAAGGG CAGTTGATGG CCTCAGATGA CTTTGGCAGA 
GACTTGGCCA GCGTGCAAGC TTTACTACGT AAGCATGAAG GCCTGGAAAG AGATCTTGCA 
GCTTTGGAGG ATAAGGTTAA GGCCCTCTGT GCAGAAGCTG ACCGTTTGCA GCAGTCTCAC 
CCTATAAATG CTTCTCAAAT TCAAGTGAAA CGGGAGGAAC TGATTGCCAA CTGGGAGCAG 
ATCCGAACTC TGGCAGCAGA GAGGCATGCT CGCCTTAATG ACTCCTACAG GTTGCAGCGC 
TTTCTTGCAG ATTTCCGGGA CCTCACTAGC TGGGTAACTG AGATGAAGGC TCTGATAAAT 
GCTGATGAGC TTGCCAATGA TGTGGCTGGA GCAGAAGCCC TTCTAGATAG ACATCAGGAA 
CATAAGGGAG AAATTGATGC TCATGAGGAT AGCTTCAGAT CTGCTGATGA GTCTGGGCAG 
GCTTTGCTTG CAGCTGGGCA CTATGCTTCT GATGAAGTTA AAGAAAAGCT GACTATCCTC 
TCAGATGAAA GATCAGCCTT GCTGGAACTA TGGGAGCTTC GCAGGCAACA GTATGAGCAG 
TGCATGGATC TGCAGCTTTT CTACAGAGAT ACTGAACAAG TTGACAACTG GATGAGCAAA 
CAAGAAGCTT TTCTGCTGAA CGAAGACCTT GGTGATTCTC TGGATAGCGT GGAGGCTCTT 
CTAAAGAAGC ATGAAGATTT TGAGAAATCC CTAAGTGCTC AAGAGGAGAA GATCACAGCA 
CTGGATGAGT TTGCTACTAA ACTGATTCAA AATAACCATT ATGCCATGGA TGACGTTGCT 
ACGCGCAGAG ATGCTCTTCT GAGTCGCCGA AATGCTCTTC ATGAAAGAGC CATGAAGCGC 
CGTGCCCAAC TGGCAGACTC TTTCCATCTC CAGCAGTTTT TCAGGGACTC TGATGAACTG 
AAGAGTTGGG TTAATGAAAA AATGAAAACT GCTACCGATG AGGCTTACAA GGATCCATCC 
AACTTGCAAG GTAAAGTTCA GAAGCATCAG GCTTTTGAAG CAGAGCTTTC TGCTAATCAG 
AGTCGTATTG ATGCACTGGA GAAAGCTGGC CAGAAGTTGA TTGATGTCAA TCACTATGCA 
TCTGATGAGG TGGCAGCTCG CATGAATGAA GTCATCAGCT TGTGGAAGAA ACTTCTGGAA 
GCCACCGAGC TCAAAGGTAT AAAGCTGCGT GAAGCCAATC AACAGCAGCA ATTTAATCGC 
AATGTAGAAG ACATTGAGTT GTGGCTGTAT GAAGTGGAGG GTCACTTGGC TTCTGATGAT 
TATGGAAAAG ATCTTACCAG CGTTCAGAAT CTTCAGAAGA AACATGCCCT GCTAGAGGCA 
GATGTTGCTG CCCATCAGGA TCGGATAGAT GGCATTACCA TCCAGGCACG CCAGTTCCAA 
GATGCTGGGC ACTTTGATGC TGACAATATC AAGAAGAAAC AAGAAGCTTT AGTAGCTCGT 
TATGAAGCTC TGAAGGATCC TATGGTGGCT CGCAAGCAGA AACTTGCAGA TTCTCTTCGC 
CTGCAGCAGC TTTTCCGTGA CATCGAGGAT GAAGAGACCT GGATCAGGGA AAAGGAACCT 
ATTGCAGCCT CAACAAACCG AGGCAAGGAC TTAATTGGTG TCCAGAATCT GCTAAAGAAG 
CACCAGGCTT TGCAGGCAGA AATTGCAGGC CATGAGCCTC GCATTAAAGC AGTCACACAA 
AAAGGAAATG CGATGGTGGA AGAGGGACAT TTTGCTGCTG AAGATGTGAA GATCAAATTG 
AATGAACTAA ACCAAAAATG GGACTCTCTG AAAGCCAAGG CATCTCAACG GCGACAGGAT 
CTAGAGGATT CTCTGCAAGC TCAGCAGTAT TTTGCTGATG CTAATGAGGC AGAATCATGG 
ATGAGGGAAA AGGAGCCCAT TGTAGGCAGC ACGGATTATG GAAAGGATGA AGACTCTGCT 
GAGGCTCTCC TGAAGAAGCA TGAAGCTTTG ATGTCTGATC TTTCTGCTTA CGGCAGTAGC 
ATACAGGCAT TAAGGGAACA GGCCCAGTCC TGCAGGCAAC AAGTTGCTCC CACTGATGAT 
GAAACTGGAA AAGAGCTTGT GCTAGCACTC TATGATTACC AAGAGAAGAG TCCTCGGGAG 
GTGACTATGA AGAAAGGAGA TATTCTAACC CTGCTCAACA GCACCAACAA GGACTGGTGG 
AAGGTTGAAG TTAACGATCG TCAGGGCTTT GTACCAGCTG CCTATGTGAA AAAACTAGAT 
CCTGCCCAGT CTGCATCCCG AGAGAATCTT CTAGAAGAGC AAGGCAGCAT AGCATTGCGA 
CAGGAGCAAA TTGACAACCA GACTCTCATT ACTAAGGAAG TCGGCAGTGT ATCTCTGCGT 
ATGAAACAGG TCGAAGAACT GTATCATTCC CTTCTTGAGC TGGGAGAAAA ACGTAAAGGC 
ATGCTAGAAA AAAGCTGCAA GAAGTTTATG CTTTTCCGTG AGGCTAATGA GCTTCAACAG 
TGGATCAATG AGAAGGAAGC TGCGCTCACT AATGAGGAAG TGGGTGCTGA TTTGGAGCAG 
GTGGAGGTGC TACAGAAGAA GTTTGATGAT TTTCAGAAGG ATTTAAAAGC TAATGAGTCA 
CGACTGAAGG ACATAAACAA GGTTGCAAAT GACCTGGAGT CTGAAGGGCT GATGGCAGAG 
GAAGTGCAAG CAGTAGAGCA CCAGGAAGTC TATGGAATGA TGCCCAGAGA TGAAACTGAT 
TCTAAGACAG CCTCTCCTTG GAAGTCTGCA CGTATGATGG TACACACAGT GGCAACGTTT 
AACTCAATCA AGGAACTGAA TGAACGCTGG AGATCCCTGC AGCAGTTGGC AGAGGAGCGA 
AGCCAGCTGT TGGGCAGCGC TCATGAAGTC CAGAGGTTCC ACAGAGATGC TGATGAAACA 
AAAGAATGGA TAGAAGAGAA GAATCAAGCA TTAAATACCG ACAACTATGG ACATGACTTG 
GCCAGTGTGC AGGCTCTGCA GCGCAAGCAT GAAGGCTTTG AGAGAGACTT GGCAGCTCTT 
GGAGACAAGG TGAATTCTCT TGGTGAAACT GCCCAGCGTC TGATCCAGTC ACATCCAGAA 
TCAGCTGAAG ATCTTCAAGA AAAATGTACT GAGTTGAACC AAGCCTGGAG TAGTCTGGGA 
AAACGTGCTG ACCAACGCAA AGAGAAGCTT GGAGATTCTC ATGACCTGCA GCGTTTCCTC 
AGTGATTTTA GAGACCTTAT GTCTTGGATC AATGGAATCC GGGGTCTGGT CTCCTCAGAT 
GAACTTGCAA AAGATGTGAC TGGAGCTGAA GCTCTGTTGG AAAGGCATCA GGAGCACCGT 
ACTGAAATAG ATGCACGGGC TGGCACTTTT CAGGCATTTG AACAGTTTGG ACAGCAGCTT 
CTGGCTCGTG GACACTATGC CAGCCCAGAG ATTAAGGAGA AACTGGATAT TCTAGATCAA 
GAACGGACAG ACCTAGAGAA GGCCTGGGTC CAGCGCAGAA TGATGCTAGA CCAGTGCTTA 
GAACTACAGC TGTTTCATCG GGATTGTGAA CAAGCTGAAA ACTGGATGGC TGCCCGAGAG 
GCGTTCCTAA ATACAGAAGA CAAAGGAGAC TCCTTAGACA GCGTGGAGGC ACTCATCAAG 
AAGCATGAAG ATTTCGATAA AGCAATCAAT GTCCAGGAAG AGAAAATTGC TGTCTTGCAG 
TCTTTTGCTG ACCAACTGAT TGCTGCTGAT CATTATGCAA AAGGAGTCAT TGCTAACAGA 
CGCAATGAGG TTCTGGACAG GTGGCGTCGT CTGAAGGCTC AGATGATTGA GAAGAGATCT 
AAGCTGGGAG AATCTCAGAC CCTCCAACAG TTCAGTCGTG ATGTTGATGA AATAGAAGCT 
TGGATCAGTG AAAAGCTTCA AACTGCAAGT GATGAGTCAT ATAAGGATCC CACAAACATC 
CAGCTTTCCA AACTGCTGAG CAAGCACCAG AAGCACCAAG CCTTTGAAGC TGAGCTCCAC 
GCCAACGCAG ATCGGATTCG TGGAGTCATT GAAATGGGGA ACTCTCTTAT TGAAAGAGGA 
GCGTGTGCTG GCAGCGAGGA TGCTGTGAAG GCTCGGCTGG CTGCCCTGGC TGACCAATGG 
GAGTTCCTGG TCCAGAAGTC ATCAGAGAAG AGTCAGAAAC TGAAAGAAGC AAATAAACAG 
CAGAATTTCA ATACCGGCAT CAAGGACTTT GATTTCTGGC TTTCAGAGGT GGAAGCTTTG 
TTGGCATCTG AAGACTATGG GAAGGACTTG GCATCAGTGA ACAACCTTCT GAAGAAGCAC 
CAATTACTGG AAGCTGATAT ATCTGCTCAT GAGGATCGGC TGAAGGACCT GAACAGCCAG 
GCTGACAGTT TGATGACCAG CAGTGCTTTC GATACCTCCC AAGTAAAGGA TAAACGTGAA 
ACTATAAATG GGCGCTTCCA GAGAATCAAG AGCATGGCAG CTGCCCGCCG TGCGAAGCTC 
AACGAGTCGC ACCGCTTGCA TCAGTTCTTC CGTGACATGG ATGATGAGGA GTCCTGGATC 
AAAGAGAAGA AACTGTTGGT GAGCTCAGAG GACTATGGCA GAGACCTGAC TGGTGTGCAG 
AACCTGAGGA AGAAACATAA GCGCTTGGAA GCAGAATTAG CTGCCCATGA ACCTGCTATC 
CAGGGTGTTC TAGACACTGG CAAGAAGCTT TCAGATGATA ACACAATTGG GAAGGAGGAG 
ATACAGCAAA GACTGGCTCA GTTTGTGGAC CACTGGAAAG AGTTAAAACA GCTGGCAGCT 
GCTCGGGGGC AGCGTCTGGA GGAGTCCCTG GAGTACCAAC AGTTTGTAGC AAATGTTGAG 
GAAGAGGAGG CATGGATCAA CGAGAAAATG ACATTGGTAG CCAGTGAGGA TTATGGAGAC 
ACACTTGCTG CTATCCAGGG CTTGCTGAAG AAGCATGAAG CATTCGAGAC TGACTTTACT 
GTCCACAAAG ACAGAGTGAA TGATGTTTGC GCTAATGGAG AGGATCTCAT TAAAAAGAAT 
AATCACCATG TGGAGAACAT TACTGCTAAG ATGAAGGGCC TCAAGGGCAA GGTATCAGAT 
CTGGAGAAAG CCGCAGCTCA GAGGAAAGCC AAACTGGATG AGAACTCTGC CTTCCTCCAG 
TTCAACTGGA AAGCAGATGT GGTGGAGTCA TGGATAGGTG AGAAGGAAAA CAGTCTGAAG 
ACAGATGATT ATGGCCGTGA CCTCTCCTCT GTGCAAACAC TACTCACCAA ACAGGAAACC 
TTTGATGCTG GACTTCAGGC TTTCCAGCAG GAGGGAATTG CAAACATCAC TGCTCTGAAA 
GACCAGCTAC TGGCAGCCAA ACATATCCAA TCAAAGGCCA TTGAGGTTCG TCACGCTTCC 
TTGATGAAAC GCTGGAATCA GCTGCTTGCT AATTCTGCAG CCAGGAAAAA GAAACTCTTG 
GAGGCTCAGG AGCACTTCAG AAAGGTTGAA GATCTCTTCT TGACTTTTGC CAAGAAGGCC 
TCTGCCTTCA ACAGTTGGTT TGAGAATGCT GAGGAGGACC TGACGGATCC CGTGCGCTGC 
AATTCCCTGG AAGAAATCAA AGCACTGCGA GAAGCCCACG ATGCGTTCCG TTCCTCACTT 
AGCTCTGCCC AAGCTGACTT CAACCAGCTG GCAGAGCTTG ATCGGCAGAT CAAGAGCTTC 
CGTGTAGCCT CCAACCCGTA CACTTGGTTT ACTATGGAGG CTCTTGAAGA AACTTGGAGG 
AATCTGCAGA AAATTATCAA GGAGCGTGAA TTGGAGTTGC AGAAGGAGCA GCGGAGGCAG 
GAAGAAAATG ACAAGTTGCG CCAGGAGTTT GCTCAGCATG CTAATGCCTT CCACCAGTGG 
ATTCAGGAGA CAAGGTCCTG CATGGTGGAG GAGTCGGGAA CACTGGAATC CCAGCTGGAA 
GCTACTAAAC GCAAGCACCA GGAGATCCGG GCTATGAGGA GCCAGCTGAA GAAGATTGAG 
GACCTTGGAG CAGCCATGGA AGAGGCACTT ATCTTGGACA ACAAATACAC AGAGCACAGC 
ACCGTGGGGC TGGCGCAGCA GTGGGACCAG CTTGACCAGC TGGGAATGAG AATGCAGCAC 
AATCTGGAAC AGCAGATTCA AGCTCGAAAC ACAACTGGAG TCACAGAGGA GGCCCTGAAG 
GAGTTCAGCA TGATGTTCAA GCACTTTGAC AAGGACAAAT CTGGACGTCT TAATCACCAG 
GAGTTTAAGT CTTGCTTGCG CTCCCTTGGC TATGACCTGC CCATGGTTGA GGAAGGAGAG 
CCTGACCCCG AATTTGAGTC TATTCTTGAC ACTGTTGATC CCAACAGGGA TGGCCACGTC 
TCGCTGCAGG AGTACATGGC GTTCATGATC AGCAGGGAAA CAGAGAACGT GAAATCCAGC 
GAGGAGATTG AGAGTGCTTT CCGTGCCCTC AGCTCGGAGG GGAAGCCTTA CGTGACCAAG 
GAGGAGCTCT ACCAGAACCT GACCCGGGAG CAGGCCGACT ACTGCATTTC TCACATGAAG 
CCCTACATGG ATGGCAAGGG CAGAGAACTT CCTTCTGCCT ACGACTACAT AGAATTTACA 
CGTTCACTCT TTGTGAATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015134774.1
CDS	17..7537
Translation	MLSSFRKVKVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSSLRRQKLEDSYRFQFFQRDADELEKWIQEKLQIASDENYKDPSNLQGKLQKHQAFEAEVQANSGAIVKLDETGNQMINEGHFASETIRSFTPYRTAQRPIVTATQVGSHIRTRLQELHRLWELLLEKMREKGVKLLQAQKLVQFLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDLAAHEERVNEVNQFAGKLIQEQHPEEELIKSKQDEVNASWQRLKGLAQQRQGKLFGAAEVQRFNRDVDETISWIKEKGQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPINASQIQVKREELIANWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFRSADESGQALLAAGHYASDEVKEKLTILSDERSALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMDDVATRRDALLSRRNALHERAMKRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYASDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTSVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDADNIKKKQEALVARYEALKDPMVARKQKLADSLRLQQLFRDIEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKIKLNELNQKWDSLKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPTDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTLITKEVGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTNEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVANDLESEGLMAEEVQAVEHQEVYGMMPRDETDSKTASPWKSARMMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAQRLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKEKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLARGHYASPEIKEKLDILDQERTDLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAVLQSFADQLIAADHYAKGVIANRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIEMGNSLIERGACAGSEDAVKARLAALADQWEFLVQKSSEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRETINGRFQRIKSMAAARRAKLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVDHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCANGEDLIKKNNHHVENITAKMKGLKGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHIQSKAIEVRHASLMKRWNQLLANSAARKKKLLEAQEHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFESILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCISHMKPYMDGKGRELPSAYDYIEFTRSLFVN

Target ORF information:

RefSeq Version	XM_015279288.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279288.1

ORF Insert Sequence:

ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG 
TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC 
TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT 
GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT 
GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG 
ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAT CTTTCACGCC TTACAGGACT 
GCACAGCGCC CAATAGTAAC AGCCACCCAA GTTGGGTCCC ACATACGAAC TCGTCTACAG 
GAGCTGCACC GACTATGGGA GTTACTGCTG GAAAAAATGA GAGAGAAGGG AGTGAAGCTG 
TTGCAAGCAC AGAAGCTGGT GCAATTCTTA CGGGAATGTG AAGATGTCAT GGACTGGATC 
AATGACAAGG AAGCAATAGT GACCTCAGAA GAGCTTGGAC AGGACTTAGA GCATGTTGAG 
GTTTTGCAGA AGAAATTTGA AGAGTTCCAA ACAGATCTGG CAGCTCATGA GGAGAGAGTG 
AATGAAGTGA ACCAGTTCGC TGGCAAACTT ATCCAGGAAC AGCACCCTGA GGAGGAACTT 
ATAAAGTCCA AACAGGATGA AGTAAATGCA AGCTGGCAGC GTCTTAAGGG GCTTGCCCAA 
CAGAGGCAAG GAAAACTCTT TGGGGCAGCT GAAGTTCAGC GCTTCAACAG GGATGTGGAT 
GAAACTATCA GCTGGATTAA GGAGAAAGGG CAGTTGATGG CCTCAGATGA CTTTGGCAGA 
GACTTGGCCA GCGTGCAAGC TTTACTACGT AAGCATGAAG GCCTGGAAAG AGATCTTGCA 
GCTTTGGAGG ATAAGGTTAA GGCCCTCTGT GCAGAAGCTG ACCGTTTGCA GCAGTCTCAC 
CCTATAAATG CTTCTCAAAT TCAAGTGAAA CGGGAGGAAC TGATTGCCAA CTGGGAGCAG 
ATCCGAACTC TGGCAGCAGA GAGGCATGCT CGCCTTAATG ACTCCTACAG GTTGCAGCGC 
TTTCTTGCAG ATTTCCGGGA CCTCACTAGC TGGGTAACTG AGATGAAGGC TCTGATAAAT 
GCTGATGAGC TTGCCAATGA TGTGGCTGGA GCAGAAGCCC TTCTAGATAG ACATCAGGAA 
CATAAGGGAG AAATTGATGC TCATGAGGAT AGCTTCAGAT CTGCTGATGA GTCTGGGCAG 
GCTTTGCTTG CAGCTGGGCA CTATGCTTCT GATGAAGTTA AAGAAAAGCT GACTATCCTC 
TCAGATGAAA GATCAGCCTT GCTGGAACTA TGGGAGCTTC GCAGGCAACA GTATGAGCAG 
TGCATGGATC TGCAGCTTTT CTACAGAGAT ACTGAACAAG TTGACAACTG GATGAGCAAA 
CAAGAAGCTT TTCTGCTGAA CGAAGACCTT GGTGATTCTC TGGATAGCGT GGAGGCTCTT 
CTAAAGAAGC ATGAAGATTT TGAGAAATCC CTAAGTGCTC AAGAGGAGAA GATCACAGCA 
CTGGATGAGT TTGCTACTAA ACTGATTCAA AATAACCATT ATGCCATGGA TGACGTTGCT 
ACGCGCAGAG ATGCTCTTCT GAGTCGCCGA AATGCTCTTC ATGAAAGAGC CATGAAGCGC 
CGTGCCCAAC TGGCAGACTC TTTCCATCTC CAGCAGTTTT TCAGGGACTC TGATGAACTG 
AAGAGTTGGG TTAATGAAAA AATGAAAACT GCTACCGATG AGGCTTACAA GGATCCATCC 
AACTTGCAAG GTAAAGTTCA GAAGCATCAG GCTTTTGAAG CAGAGCTTTC TGCTAATCAG 
AGTCGTATTG ATGCACTGGA GAAAGCTGGC CAGAAGTTGA TTGATGTCAA TCACTATGCA 
TCTGATGAGG TGGCAGCTCG CATGAATGAA GTCATCAGCT TGTGGAAGAA ACTTCTGGAA 
GCCACCGAGC TCAAAGGTAT AAAGCTGCGT GAAGCCAATC AACAGCAGCA ATTTAATCGC 
AATGTAGAAG ACATTGAGTT GTGGCTGTAT GAAGTGGAGG GTCACTTGGC TTCTGATGAT 
TATGGAAAAG ATCTTACCAG CGTTCAGAAT CTTCAGAAGA AACATGCCCT GCTAGAGGCA 
GATGTTGCTG CCCATCAGGA TCGGATAGAT GGCATTACCA TCCAGGCACG CCAGTTCCAA 
GATGCTGGGC ACTTTGATGC TGACAATATC AAGAAGAAAC AAGAAGCTTT AGTAGCTCGT 
TATGAAGCTC TGAAGGATCC TATGGTGGCT CGCAAGCAGA AACTTGCAGA TTCTCTTCGC 
CTGCAGCAGC TTTTCCGTGA CATCGAGGAT GAAGAGACCT GGATCAGGGA AAAGGAACCT 
ATTGCAGCCT CAACAAACCG AGGCAAGGAC TTAATTGGTG TCCAGAATCT GCTAAAGAAG 
CACCAGGCTT TGCAGGCAGA AATTGCAGGC CATGAGCCTC GCATTAAAGC AGTCACACAA 
AAAGGAAATG CGATGGTGGA AGAGGGACAT TTTGCTGCTG AAGATGTGAA GATCAAATTG 
AATGAACTAA ACCAAAAATG GGACTCTCTG AAAGCCAAGG CATCTCAACG GCGACAGGAT 
CTAGAGGATT CTCTGCAAGC TCAGCAGTAT TTTGCTGATG CTAATGAGGC AGAATCATGG 
ATGAGGGAAA AGGAGCCCAT TGTAGGCAGC ACGGATTATG GAAAGGATGA AGACTCTGCT 
GAGGCTCTCC TGAAGAAGCA TGAAGCTTTG ATGTCTGATC TTTCTGCTTA CGGCAGTAGC 
ATACAGGCAT TAAGGGAACA GGCCCAGTCC TGCAGGCAAC AAGTTGCTCC CACTGATGAT 
GAAACTGGAA AAGAGCTTGT GCTAGCACTC TATGATTACC AAGAGAAGAG TCCTCGGGAG 
GTGACTATGA AGAAAGGAGA TATTCTAACC CTGCTCAACA GCACCAACAA GGACTGGTGG 
AAGGTTGAAG TTAACGATCG TCAGGGCTTT GTACCAGCTG CCTATGTGAA AAAACTAGAT 
CCTGCCCAGT CTGCATCCCG AGAGAATCTT CTAGAAGAGC AAGGCAGCAT AGCATTGCGA 
CAGGAGCAAA TTGACAACCA GACTCTCATT ACTAAGGAAG TCGGCAGTGT ATCTCTGCGT 
ATGAAACAGG TCGAAGAACT GTATCATTCC CTTCTTGAGC TGGGAGAAAA ACGTAAAGGC 
ATGCTAGAAA AAAGCTGCAA GAAGTTTATG CTTTTCCGTG AGGCTAATGA GCTTCAACAG 
TGGATCAATG AGAAGGAAGC TGCGCTCACT AATGAGGAAG TGGGTGCTGA TTTGGAGCAG 
GTGGAGGTGC TACAGAAGAA GTTTGATGAT TTTCAGAAGG ATTTAAAAGC TAATGAGTCA 
CGACTGAAGG ACATAAACAA GGTTGCAAAT GACCTGGAGT CTGAAGGGCT GATGGCAGAG 
GAAGTGCAAG CAGTAGAGCA CCAGGAAGTC TATGGAATGA TGCCCAGAGA TGAAACTGAT 
TCTAAGACAG CCTCTCCTTG GAAGTCTGCA CGTATGATGG TACACACAGT GGCAACGTTT 
AACTCAATCA AGGAACTGAA TGAACGCTGG AGATCCCTGC AGCAGTTGGC AGAGGAGCGA 
AGCCAGCTGT TGGGCAGCGC TCATGAAGTC CAGAGGTTCC ACAGAGATGC TGATGAAACA 
AAAGAATGGA TAGAAGAGAA GAATCAAGCA TTAAATACCG ACAACTATGG ACATGACTTG 
GCCAGTGTGC AGGCTCTGCA GCGCAAGCAT GAAGGCTTTG AGAGAGACTT GGCAGCTCTT 
GGAGACAAGG TGAATTCTCT TGGTGAAACT GCCCAGCGTC TGATCCAGTC ACATCCAGAA 
TCAGCTGAAG ATCTTCAAGA AAAATGTACT GAGTTGAACC AAGCCTGGAG TAGTCTGGGA 
AAACGTGCTG ACCAACGCAA AGAGAAGCTT GGAGATTCTC ATGACCTGCA GCGTTTCCTC 
AGTGATTTTA GAGACCTTAT GTCTTGGATC AATGGAATCC GGGGTCTGGT CTCCTCAGAT 
GAACTTGCAA AAGATGTGAC TGGAGCTGAA GCTCTGTTGG AAAGGCATCA GGAGCACCGT 
ACTGAAATAG ATGCACGGGC TGGCACTTTT CAGGCATTTG AACAGTTTGG ACAGCAGCTT 
CTGGCTCGTG GACACTATGC CAGCCCAGAG ATTAAGGAGA AACTGGATAT TCTAGATCAA 
GAACGGACAG ACCTAGAGAA GGCCTGGGTC CAGCGCAGAA TGATGCTAGA CCAGTGCTTA 
GAACTACAGC TGTTTCATCG GGATTGTGAA CAAGCTGAAA ACTGGATGGC TGCCCGAGAG 
GCGTTCCTAA ATACAGAAGA CAAAGGAGAC TCCTTAGACA GCGTGGAGGC ACTCATCAAG 
AAGCATGAAG ATTTCGATAA AGCAATCAAT GTCCAGGAAG AGAAAATTGC TGTCTTGCAG 
TCTTTTGCTG ACCAACTGAT TGCTGCTGAT CATTATGCAA AAGGAGTCAT TGCTAACAGA 
CGCAATGAGG TTCTGGACAG GTGGCGTCGT CTGAAGGCTC AGATGATTGA GAAGAGATCT 
AAGCTGGGAG AATCTCAGAC CCTCCAACAG TTCAGTCGTG ATGTTGATGA AATAGAAGCT 
TGGATCAGTG AAAAGCTTCA AACTGCAAGT GATGAGTCAT ATAAGGATCC CACAAACATC 
CAGCTTTCCA AACTGCTGAG CAAGCACCAG AAGCACCAAG CCTTTGAAGC TGAGCTCCAC 
GCCAACGCAG ATCGGATTCG TGGAGTCATT GAAATGGGGA ACTCTCTTAT TGAAAGAGGA 
GCGTGTGCTG GCAGCGAGGA TGCTGTGAAG GCTCGGCTGG CTGCCCTGGC TGACCAATGG 
GAGTTCCTGG TCCAGAAGTC ATCAGAGAAG AGTCAGAAAC TGAAAGAAGC AAATAAACAG 
CAGAATTTCA ATACCGGCAT CAAGGACTTT GATTTCTGGC TTTCAGAGGT GGAAGCTTTG 
TTGGCATCTG AAGACTATGG GAAGGACTTG GCATCAGTGA ACAACCTTCT GAAGAAGCAC 
CAATTACTGG AAGCTGATAT ATCTGCTCAT GAGGATCGGC TGAAGGACCT GAACAGCCAG 
GCTGACAGTT TGATGACCAG CAGTGCTTTC GATACCTCCC AAGTAAAGGA TAAACGTGAA 
ACTATAAATG GGCGCTTCCA GAGAATCAAG AGCATGGCAG CTGCCCGCCG TGCGAAGCTC 
AACGAGTCGC ACCGCTTGCA TCAGTTCTTC CGTGACATGG ATGATGAGGA GTCCTGGATC 
AAAGAGAAGA AACTGTTGGT GAGCTCAGAG GACTATGGCA GAGACCTGAC TGGTGTGCAG 
AACCTGAGGA AGAAACATAA GCGCTTGGAA GCAGAATTAG CTGCCCATGA ACCTGCTATC 
CAGGGTGTTC TAGACACTGG CAAGAAGCTT TCAGATGATA ACACAATTGG GAAGGAGGAG 
ATACAGCAAA GACTGGCTCA GTTTGTGGAC CACTGGAAAG AGTTAAAACA GCTGGCAGCT 
GCTCGGGGGC AGCGTCTGGA GGAGTCCCTG GAGTACCAAC AGTTTGTAGC AAATGTTGAG 
GAAGAGGAGG CATGGATCAA CGAGAAAATG ACATTGGTAG CCAGTGAGGA TTATGGAGAC 
ACACTTGCTG CTATCCAGGG CTTGCTGAAG AAGCATGAAG CATTCGAGAC TGACTTTACT 
GTCCACAAAG ACAGAGTGAA TGATGTTTGC GCTAATGGAG AGGATCTCAT TAAAAAGAAT 
AATCACCATG TGGAGAACAT TACTGCTAAG ATGAAGGGCC TCAAGGGCAA GGTATCAGAT 
CTGGAGAAAG CCGCAGCTCA GAGGAAAGCC AAACTGGATG AGAACTCTGC CTTCCTCCAG 
TTCAACTGGA AAGCAGATGT GGTGGAGTCA TGGATAGGTG AGAAGGAAAA CAGTCTGAAG 
ACAGATGATT ATGGCCGTGA CCTCTCCTCT GTGCAAACAC TACTCACCAA ACAGGAAACC 
TTTGATGCTG GACTTCAGGC TTTCCAGCAG GAGGGAATTG CAAACATCAC TGCTCTGAAA 
GACCAGCTAC TGGCAGCCAA ACATATCCAA TCAAAGGCCA TTGAGGTTCG TCACGCTTCC 
TTGATGAAAC GCTGGAATCA GCTGCTTGCT AATTCTGCAG CCAGGAAAAA GAAACTCTTG 
GAGGCTCAGG AGCACTTCAG AAAGGTTGAA GATCTCTTCT TGACTTTTGC CAAGAAGGCC 
TCTGCCTTCA ACAGTTGGTT TGAGAATGCT GAGGAGGACC TGACGGATCC CGTGCGCTGC 
AATTCCCTGG AAGAAATCAA AGCACTGCGA GAAGCCCACG ATGCGTTCCG TTCCTCACTT 
AGCTCTGCCC AAGCTGACTT CAACCAGCTG GCAGAGCTTG ATCGGCAGAT CAAGAGCTTC 
CGTGTAGCCT CCAACCCGTA CACTTGGTTT ACTATGGAGG CTCTTGAAGA AACTTGGAGG 
AATCTGCAGA AAATTATCAA GGAGCGTGAA TTGGAGTTGC AGAAGGAGCA GCGGAGGCAG 
GAAGAAAATG ACAAGTTGCG CCAGGAGTTT GCTCAGCATG CTAATGCCTT CCACCAGTGG 
ATTCAGGAGA CAAGGTCCTG CATGGTGGAG GAGTCGGGAA CACTGGAATC CCAGCTGGAA 
GCTACTAAAC GCAAGCACCA GGAGATCCGG GCTATGAGGA GCCAGCTGAA GAAGATTGAG 
GACCTTGGAG CAGCCATGGA AGAGGCACTT ATCTTGGACA ACAAATACAC AGAGCACAGC 
ACCGTGGGGC TGGCGCAGCA GTGGGACCAG CTTGACCAGC TGGGAATGAG AATGCAGCAC 
AATCTGGAAC AGCAGATTCA AGCTCGAAAC ACAACTGGAG TCACAGAGGA GGCCCTGAAG 
GAGTTCAGCA TGATGTTCAA GCACTTTGAC AAGGACAAAT CTGGACGTCT TAATCACCAG 
GAGTTTAAGT CTTGCTTGCG CTCCCTTGGC TATGACCTGC CCATGGTTGA GGAAGGAGAG 
CCTGACCCCG AATTTGAGTC TATTCTTGAC ACTGTTGATC CCAACAGGGA TGGCCACGTC 
TCGCTGCAGG AGTACATGGC GTTCATGATC AGCAGGGAAA CAGAGAACGT GAAATCCAGC 
GAGGAGATTG AGAGTGCTTT CCGTGCCCTC AGCTCGGAGG GGAAGCCTTA CGTGACCAAG 
GAGGAGCTCT ACCAGAACCT GACCCGGGAG CAGGCCGACT ACTGCATTTC TCACATGAAG 
CCCTACATGG ATGGCAAGGG CAGAGAACTT CCTTCTGCCT ACGACTACAT AGAATTTACA 
CGTTCACTCT TTGTGAATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_015279288.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015134774.1 Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X7, mRNA.

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CloneID	OGa43314
Clone ID Related Accession (Same CDS sequence)	XM_015279288.1 , XM_015279288.2
Accession Version	XM_015279288.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7521bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015279288.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG 
TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC 
TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT 
GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT 
GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG 
ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAT CTTTCACGCC TTACAGGACT 
GCACAGCGCC CAATAGTAAC AGCCACCCAA GTTGGGTCCC ACATACGAAC TCGTCTACAG 
GAGCTGCACC GACTATGGGA GTTACTGCTG GAAAAAATGA GAGAGAAGGG AGTGAAGCTG 
TTGCAAGCAC AGAAGCTGGT GCAATTCTTA CGGGAATGTG AAGATGTCAT GGACTGGATC 
AATGACAAGG AAGCAATAGT GACCTCAGAA GAGCTTGGAC AGGACTTAGA GCATGTTGAG 
GTTTTGCAGA AGAAATTTGA AGAGTTCCAA ACAGATCTGG CAGCTCATGA GGAGAGAGTG 
AATGAAGTGA ACCAGTTCGC TGGCAAACTT ATCCAGGAAC AGCACCCTGA GGAGGAACTT 
ATAAAGTCCA AACAGGATGA AGTAAATGCA AGCTGGCAGC GTCTTAAGGG GCTTGCCCAA 
CAGAGGCAAG GAAAACTCTT TGGGGCAGCT GAAGTTCAGC GCTTCAACAG GGATGTGGAT 
GAAACTATCA GCTGGATTAA GGAGAAAGGG CAGTTGATGG CCTCAGATGA CTTTGGCAGA 
GACTTGGCCA GCGTGCAAGC TTTACTACGT AAGCATGAAG GCCTGGAAAG AGATCTTGCA 
GCTTTGGAGG ATAAGGTTAA GGCCCTCTGT GCAGAAGCTG ACCGTTTGCA GCAGTCTCAC 
CCTATAAATG CTTCTCAAAT TCAAGTGAAA CGGGAGGAAC TGATTGCCAA CTGGGAGCAG 
ATCCGAACTC TGGCAGCAGA GAGGCATGCT CGCCTTAATG ACTCCTACAG GTTGCAGCGC 
TTTCTTGCAG ATTTCCGGGA CCTCACTAGC TGGGTAACTG AGATGAAGGC TCTGATAAAT 
GCTGATGAGC TTGCCAATGA TGTGGCTGGA GCAGAAGCCC TTCTAGATAG ACATCAGGAA 
CATAAGGGAG AAATTGATGC TCATGAGGAT AGCTTCAGAT CTGCTGATGA GTCTGGGCAG 
GCTTTGCTTG CAGCTGGGCA CTATGCTTCT GATGAAGTTA AAGAAAAGCT GACTATCCTC 
TCAGATGAAA GATCAGCCTT GCTGGAACTA TGGGAGCTTC GCAGGCAACA GTATGAGCAG 
TGCATGGATC TGCAGCTTTT CTACAGAGAT ACTGAACAAG TTGACAACTG GATGAGCAAA 
CAAGAAGCTT TTCTGCTGAA CGAAGACCTT GGTGATTCTC TGGATAGCGT GGAGGCTCTT 
CTAAAGAAGC ATGAAGATTT TGAGAAATCC CTAAGTGCTC AAGAGGAGAA GATCACAGCA 
CTGGATGAGT TTGCTACTAA ACTGATTCAA AATAACCATT ATGCCATGGA TGACGTTGCT 
ACGCGCAGAG ATGCTCTTCT GAGTCGCCGA AATGCTCTTC ATGAAAGAGC CATGAAGCGC 
CGTGCCCAAC TGGCAGACTC TTTCCATCTC CAGCAGTTTT TCAGGGACTC TGATGAACTG 
AAGAGTTGGG TTAATGAAAA AATGAAAACT GCTACCGATG AGGCTTACAA GGATCCATCC 
AACTTGCAAG GTAAAGTTCA GAAGCATCAG GCTTTTGAAG CAGAGCTTTC TGCTAATCAG 
AGTCGTATTG ATGCACTGGA GAAAGCTGGC CAGAAGTTGA TTGATGTCAA TCACTATGCA 
TCTGATGAGG TGGCAGCTCG CATGAATGAA GTCATCAGCT TGTGGAAGAA ACTTCTGGAA 
GCCACCGAGC TCAAAGGTAT AAAGCTGCGT GAAGCCAATC AACAGCAGCA ATTTAATCGC 
AATGTAGAAG ACATTGAGTT GTGGCTGTAT GAAGTGGAGG GTCACTTGGC TTCTGATGAT 
TATGGAAAAG ATCTTACCAG CGTTCAGAAT CTTCAGAAGA AACATGCCCT GCTAGAGGCA 
GATGTTGCTG CCCATCAGGA TCGGATAGAT GGCATTACCA TCCAGGCACG CCAGTTCCAA 
GATGCTGGGC ACTTTGATGC TGACAATATC AAGAAGAAAC AAGAAGCTTT AGTAGCTCGT 
TATGAAGCTC TGAAGGATCC TATGGTGGCT CGCAAGCAGA AACTTGCAGA TTCTCTTCGC 
CTGCAGCAGC TTTTCCGTGA CATCGAGGAT GAAGAGACCT GGATCAGGGA AAAGGAACCT 
ATTGCAGCCT CAACAAACCG AGGCAAGGAC TTAATTGGTG TCCAGAATCT GCTAAAGAAG 
CACCAGGCTT TGCAGGCAGA AATTGCAGGC CATGAGCCTC GCATTAAAGC AGTCACACAA 
AAAGGAAATG CGATGGTGGA AGAGGGACAT TTTGCTGCTG AAGATGTGAA GATCAAATTG 
AATGAACTAA ACCAAAAATG GGACTCTCTG AAAGCCAAGG CATCTCAACG GCGACAGGAT 
CTAGAGGATT CTCTGCAAGC TCAGCAGTAT TTTGCTGATG CTAATGAGGC AGAATCATGG 
ATGAGGGAAA AGGAGCCCAT TGTAGGCAGC ACGGATTATG GAAAGGATGA AGACTCTGCT 
GAGGCTCTCC TGAAGAAGCA TGAAGCTTTG ATGTCTGATC TTTCTGCTTA CGGCAGTAGC 
ATACAGGCAT TAAGGGAACA GGCCCAGTCC TGCAGGCAAC AAGTTGCTCC CACTGATGAT 
GAAACTGGAA AAGAGCTTGT GCTAGCACTC TATGATTACC AAGAGAAGAG TCCTCGGGAG 
GTGACTATGA AGAAAGGAGA TATTCTAACC CTGCTCAACA GCACCAACAA GGACTGGTGG 
AAGGTTGAAG TTAACGATCG TCAGGGCTTT GTACCAGCTG CCTATGTGAA AAAACTAGAT 
CCTGCCCAGT CTGCATCCCG AGAGAATCTT CTAGAAGAGC AAGGCAGCAT AGCATTGCGA 
CAGGAGCAAA TTGACAACCA GACTCTCATT ACTAAGGAAG TCGGCAGTGT ATCTCTGCGT 
ATGAAACAGG TCGAAGAACT GTATCATTCC CTTCTTGAGC TGGGAGAAAA ACGTAAAGGC 
ATGCTAGAAA AAAGCTGCAA GAAGTTTATG CTTTTCCGTG AGGCTAATGA GCTTCAACAG 
TGGATCAATG AGAAGGAAGC TGCGCTCACT AATGAGGAAG TGGGTGCTGA TTTGGAGCAG 
GTGGAGGTGC TACAGAAGAA GTTTGATGAT TTTCAGAAGG ATTTAAAAGC TAATGAGTCA 
CGACTGAAGG ACATAAACAA GGTTGCAAAT GACCTGGAGT CTGAAGGGCT GATGGCAGAG 
GAAGTGCAAG CAGTAGAGCA CCAGGAAGTC TATGGAATGA TGCCCAGAGA TGAAACTGAT 
TCTAAGACAG CCTCTCCTTG GAAGTCTGCA CGTATGATGG TACACACAGT GGCAACGTTT 
AACTCAATCA AGGAACTGAA TGAACGCTGG AGATCCCTGC AGCAGTTGGC AGAGGAGCGA 
AGCCAGCTGT TGGGCAGCGC TCATGAAGTC CAGAGGTTCC ACAGAGATGC TGATGAAACA 
AAAGAATGGA TAGAAGAGAA GAATCAAGCA TTAAATACCG ACAACTATGG ACATGACTTG 
GCCAGTGTGC AGGCTCTGCA GCGCAAGCAT GAAGGCTTTG AGAGAGACTT GGCAGCTCTT 
GGAGACAAGG TGAATTCTCT TGGTGAAACT GCCCAGCGTC TGATCCAGTC ACATCCAGAA 
TCAGCTGAAG ATCTTCAAGA AAAATGTACT GAGTTGAACC AAGCCTGGAG TAGTCTGGGA 
AAACGTGCTG ACCAACGCAA AGAGAAGCTT GGAGATTCTC ATGACCTGCA GCGTTTCCTC 
AGTGATTTTA GAGACCTTAT GTCTTGGATC AATGGAATCC GGGGTCTGGT CTCCTCAGAT 
GAACTTGCAA AAGATGTGAC TGGAGCTGAA GCTCTGTTGG AAAGGCATCA GGAGCACCGT 
ACTGAAATAG ATGCACGGGC TGGCACTTTT CAGGCATTTG AACAGTTTGG ACAGCAGCTT 
CTGGCTCGTG GACACTATGC CAGCCCAGAG ATTAAGGAGA AACTGGATAT TCTAGATCAA 
GAACGGACAG ACCTAGAGAA GGCCTGGGTC CAGCGCAGAA TGATGCTAGA CCAGTGCTTA 
GAACTACAGC TGTTTCATCG GGATTGTGAA CAAGCTGAAA ACTGGATGGC TGCCCGAGAG 
GCGTTCCTAA ATACAGAAGA CAAAGGAGAC TCCTTAGACA GCGTGGAGGC ACTCATCAAG 
AAGCATGAAG ATTTCGATAA AGCAATCAAT GTCCAGGAAG AGAAAATTGC TGTCTTGCAG 
TCTTTTGCTG ACCAACTGAT TGCTGCTGAT CATTATGCAA AAGGAGTCAT TGCTAACAGA 
CGCAATGAGG TTCTGGACAG GTGGCGTCGT CTGAAGGCTC AGATGATTGA GAAGAGATCT 
AAGCTGGGAG AATCTCAGAC CCTCCAACAG TTCAGTCGTG ATGTTGATGA AATAGAAGCT 
TGGATCAGTG AAAAGCTTCA AACTGCAAGT GATGAGTCAT ATAAGGATCC CACAAACATC 
CAGCTTTCCA AACTGCTGAG CAAGCACCAG AAGCACCAAG CCTTTGAAGC TGAGCTCCAC 
GCCAACGCAG ATCGGATTCG TGGAGTCATT GAAATGGGGA ACTCTCTTAT TGAAAGAGGA 
GCGTGTGCTG GCAGCGAGGA TGCTGTGAAG GCTCGGCTGG CTGCCCTGGC TGACCAATGG 
GAGTTCCTGG TCCAGAAGTC ATCAGAGAAG AGTCAGAAAC TGAAAGAAGC AAATAAACAG 
CAGAATTTCA ATACCGGCAT CAAGGACTTT GATTTCTGGC TTTCAGAGGT GGAAGCTTTG 
TTGGCATCTG AAGACTATGG GAAGGACTTG GCATCAGTGA ACAACCTTCT GAAGAAGCAC 
CAATTACTGG AAGCTGATAT ATCTGCTCAT GAGGATCGGC TGAAGGACCT GAACAGCCAG 
GCTGACAGTT TGATGACCAG CAGTGCTTTC GATACCTCCC AAGTAAAGGA TAAACGTGAA 
ACTATAAATG GGCGCTTCCA GAGAATCAAG AGCATGGCAG CTGCCCGCCG TGCGAAGCTC 
AACGAGTCGC ACCGCTTGCA TCAGTTCTTC CGTGACATGG ATGATGAGGA GTCCTGGATC 
AAAGAGAAGA AACTGTTGGT GAGCTCAGAG GACTATGGCA GAGACCTGAC TGGTGTGCAG 
AACCTGAGGA AGAAACATAA GCGCTTGGAA GCAGAATTAG CTGCCCATGA ACCTGCTATC 
CAGGGTGTTC TAGACACTGG CAAGAAGCTT TCAGATGATA ACACAATTGG GAAGGAGGAG 
ATACAGCAAA GACTGGCTCA GTTTGTGGAC CACTGGAAAG AGTTAAAACA GCTGGCAGCT 
GCTCGGGGGC AGCGTCTGGA GGAGTCCCTG GAGTACCAAC AGTTTGTAGC AAATGTTGAG 
GAAGAGGAGG CATGGATCAA CGAGAAAATG ACATTGGTAG CCAGTGAGGA TTATGGAGAC 
ACACTTGCTG CTATCCAGGG CTTGCTGAAG AAGCATGAAG CATTCGAGAC TGACTTTACT 
GTCCACAAAG ACAGAGTGAA TGATGTTTGC GCTAATGGAG AGGATCTCAT TAAAAAGAAT 
AATCACCATG TGGAGAACAT TACTGCTAAG ATGAAGGGCC TCAAGGGCAA GGTATCAGAT 
CTGGAGAAAG CCGCAGCTCA GAGGAAAGCC AAACTGGATG AGAACTCTGC CTTCCTCCAG 
TTCAACTGGA AAGCAGATGT GGTGGAGTCA TGGATAGGTG AGAAGGAAAA CAGTCTGAAG 
ACAGATGATT ATGGCCGTGA CCTCTCCTCT GTGCAAACAC TACTCACCAA ACAGGAAACC 
TTTGATGCTG GACTTCAGGC TTTCCAGCAG GAGGGAATTG CAAACATCAC TGCTCTGAAA 
GACCAGCTAC TGGCAGCCAA ACATATCCAA TCAAAGGCCA TTGAGGTTCG TCACGCTTCC 
TTGATGAAAC GCTGGAATCA GCTGCTTGCT AATTCTGCAG CCAGGAAAAA GAAACTCTTG 
GAGGCTCAGG AGCACTTCAG AAAGGTTGAA GATCTCTTCT TGACTTTTGC CAAGAAGGCC 
TCTGCCTTCA ACAGTTGGTT TGAGAATGCT GAGGAGGACC TGACGGATCC CGTGCGCTGC 
AATTCCCTGG AAGAAATCAA AGCACTGCGA GAAGCCCACG ATGCGTTCCG TTCCTCACTT 
AGCTCTGCCC AAGCTGACTT CAACCAGCTG GCAGAGCTTG ATCGGCAGAT CAAGAGCTTC 
CGTGTAGCCT CCAACCCGTA CACTTGGTTT ACTATGGAGG CTCTTGAAGA AACTTGGAGG 
AATCTGCAGA AAATTATCAA GGAGCGTGAA TTGGAGTTGC AGAAGGAGCA GCGGAGGCAG 
GAAGAAAATG ACAAGTTGCG CCAGGAGTTT GCTCAGCATG CTAATGCCTT CCACCAGTGG 
ATTCAGGAGA CAAGGTCCTG CATGGTGGAG GAGTCGGGAA CACTGGAATC CCAGCTGGAA 
GCTACTAAAC GCAAGCACCA GGAGATCCGG GCTATGAGGA GCCAGCTGAA GAAGATTGAG 
GACCTTGGAG CAGCCATGGA AGAGGCACTT ATCTTGGACA ACAAATACAC AGAGCACAGC 
ACCGTGGGGC TGGCGCAGCA GTGGGACCAG CTTGACCAGC TGGGAATGAG AATGCAGCAC 
AATCTGGAAC AGCAGATTCA AGCTCGAAAC ACAACTGGAG TCACAGAGGA GGCCCTGAAG 
GAGTTCAGCA TGATGTTCAA GCACTTTGAC AAGGACAAAT CTGGACGTCT TAATCACCAG 
GAGTTTAAGT CTTGCTTGCG CTCCCTTGGC TATGACCTGC CCATGGTTGA GGAAGGAGAG 
CCTGACCCCG AATTTGAGTC TATTCTTGAC ACTGTTGATC CCAACAGGGA TGGCCACGTC 
TCGCTGCAGG AGTACATGGC GTTCATGATC AGCAGGGAAA CAGAGAACGT GAAATCCAGC 
GAGGAGATTG AGAGTGCTTT CCGTGCCCTC AGCTCGGAGG GGAAGCCTTA CGTGACCAAG 
GAGGAGCTCT ACCAGAACCT GACCCGGGAG CAGGCCGACT ACTGCATTTC TCACATGAAG 
CCCTACATGG ATGGCAAGGG CAGAGAACTT CCTTCTGCCT ACGACTACAT AGAATTTACA 
CGTTCACTCT TTGTGAATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015134774.1
CDS	18..7538
Translation	MLSSFRKVKVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSSLRRQKLEDSYRFQFFQRDADELEKWIQEKLQIASDENYKDPSNLQGKLQKHQAFEAEVQANSGAIVKLDETGNQMINEGHFASETIRSFTPYRTAQRPIVTATQVGSHIRTRLQELHRLWELLLEKMREKGVKLLQAQKLVQFLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDLAAHEERVNEVNQFAGKLIQEQHPEEELIKSKQDEVNASWQRLKGLAQQRQGKLFGAAEVQRFNRDVDETISWIKEKGQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPINASQIQVKREELIANWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFRSADESGQALLAAGHYASDEVKEKLTILSDERSALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMDDVATRRDALLSRRNALHERAMKRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYASDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTSVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDADNIKKKQEALVARYEALKDPMVARKQKLADSLRLQQLFRDIEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKIKLNELNQKWDSLKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPTDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTLITKEVGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTNEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVANDLESEGLMAEEVQAVEHQEVYGMMPRDETDSKTASPWKSARMMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAQRLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKEKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLARGHYASPEIKEKLDILDQERTDLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAVLQSFADQLIAADHYAKGVIANRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIEMGNSLIERGACAGSEDAVKARLAALADQWEFLVQKSSEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRETINGRFQRIKSMAAARRAKLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVDHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCANGEDLIKKNNHHVENITAKMKGLKGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHIQSKAIEVRHASLMKRWNQLLANSAARKKKLLEAQEHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFESILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCISHMKPYMDGKGRELPSAYDYIEFTRSLFVN

Target ORF information:

RefSeq Version	XM_015279288.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279288.2

ORF Insert Sequence:

ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG 
TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC 
TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT 
GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT 
GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG 
ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAT CTTTCACGCC TTACAGGACT 
GCACAGCGCC CAATAGTAAC AGCCACCCAA GTTGGGTCCC ACATACGAAC TCGTCTACAG 
GAGCTGCACC GACTATGGGA GTTACTGCTG GAAAAAATGA GAGAGAAGGG AGTGAAGCTG 
TTGCAAGCAC AGAAGCTGGT GCAATTCTTA CGGGAATGTG AAGATGTCAT GGACTGGATC 
AATGACAAGG AAGCAATAGT GACCTCAGAA GAGCTTGGAC AGGACTTAGA GCATGTTGAG 
GTTTTGCAGA AGAAATTTGA AGAGTTCCAA ACAGATCTGG CAGCTCATGA GGAGAGAGTG 
AATGAAGTGA ACCAGTTCGC TGGCAAACTT ATCCAGGAAC AGCACCCTGA GGAGGAACTT 
ATAAAGTCCA AACAGGATGA AGTAAATGCA AGCTGGCAGC GTCTTAAGGG GCTTGCCCAA 
CAGAGGCAAG GAAAACTCTT TGGGGCAGCT GAAGTTCAGC GCTTCAACAG GGATGTGGAT 
GAAACTATCA GCTGGATTAA GGAGAAAGGG CAGTTGATGG CCTCAGATGA CTTTGGCAGA 
GACTTGGCCA GCGTGCAAGC TTTACTACGT AAGCATGAAG GCCTGGAAAG AGATCTTGCA 
GCTTTGGAGG ATAAGGTTAA GGCCCTCTGT GCAGAAGCTG ACCGTTTGCA GCAGTCTCAC 
CCTATAAATG CTTCTCAAAT TCAAGTGAAA CGGGAGGAAC TGATTGCCAA CTGGGAGCAG 
ATCCGAACTC TGGCAGCAGA GAGGCATGCT CGCCTTAATG ACTCCTACAG GTTGCAGCGC 
TTTCTTGCAG ATTTCCGGGA CCTCACTAGC TGGGTAACTG AGATGAAGGC TCTGATAAAT 
GCTGATGAGC TTGCCAATGA TGTGGCTGGA GCAGAAGCCC TTCTAGATAG ACATCAGGAA 
CATAAGGGAG AAATTGATGC TCATGAGGAT AGCTTCAGAT CTGCTGATGA GTCTGGGCAG 
GCTTTGCTTG CAGCTGGGCA CTATGCTTCT GATGAAGTTA AAGAAAAGCT GACTATCCTC 
TCAGATGAAA GATCAGCCTT GCTGGAACTA TGGGAGCTTC GCAGGCAACA GTATGAGCAG 
TGCATGGATC TGCAGCTTTT CTACAGAGAT ACTGAACAAG TTGACAACTG GATGAGCAAA 
CAAGAAGCTT TTCTGCTGAA CGAAGACCTT GGTGATTCTC TGGATAGCGT GGAGGCTCTT 
CTAAAGAAGC ATGAAGATTT TGAGAAATCC CTAAGTGCTC AAGAGGAGAA GATCACAGCA 
CTGGATGAGT TTGCTACTAA ACTGATTCAA AATAACCATT ATGCCATGGA TGACGTTGCT 
ACGCGCAGAG ATGCTCTTCT GAGTCGCCGA AATGCTCTTC ATGAAAGAGC CATGAAGCGC 
CGTGCCCAAC TGGCAGACTC TTTCCATCTC CAGCAGTTTT TCAGGGACTC TGATGAACTG 
AAGAGTTGGG TTAATGAAAA AATGAAAACT GCTACCGATG AGGCTTACAA GGATCCATCC 
AACTTGCAAG GTAAAGTTCA GAAGCATCAG GCTTTTGAAG CAGAGCTTTC TGCTAATCAG 
AGTCGTATTG ATGCACTGGA GAAAGCTGGC CAGAAGTTGA TTGATGTCAA TCACTATGCA 
TCTGATGAGG TGGCAGCTCG CATGAATGAA GTCATCAGCT TGTGGAAGAA ACTTCTGGAA 
GCCACCGAGC TCAAAGGTAT AAAGCTGCGT GAAGCCAATC AACAGCAGCA ATTTAATCGC 
AATGTAGAAG ACATTGAGTT GTGGCTGTAT GAAGTGGAGG GTCACTTGGC TTCTGATGAT 
TATGGAAAAG ATCTTACCAG CGTTCAGAAT CTTCAGAAGA AACATGCCCT GCTAGAGGCA 
GATGTTGCTG CCCATCAGGA TCGGATAGAT GGCATTACCA TCCAGGCACG CCAGTTCCAA 
GATGCTGGGC ACTTTGATGC TGACAATATC AAGAAGAAAC AAGAAGCTTT AGTAGCTCGT 
TATGAAGCTC TGAAGGATCC TATGGTGGCT CGCAAGCAGA AACTTGCAGA TTCTCTTCGC 
CTGCAGCAGC TTTTCCGTGA CATCGAGGAT GAAGAGACCT GGATCAGGGA AAAGGAACCT 
ATTGCAGCCT CAACAAACCG AGGCAAGGAC TTAATTGGTG TCCAGAATCT GCTAAAGAAG 
CACCAGGCTT TGCAGGCAGA AATTGCAGGC CATGAGCCTC GCATTAAAGC AGTCACACAA 
AAAGGAAATG CGATGGTGGA AGAGGGACAT TTTGCTGCTG AAGATGTGAA GATCAAATTG 
AATGAACTAA ACCAAAAATG GGACTCTCTG AAAGCCAAGG CATCTCAACG GCGACAGGAT 
CTAGAGGATT CTCTGCAAGC TCAGCAGTAT TTTGCTGATG CTAATGAGGC AGAATCATGG 
ATGAGGGAAA AGGAGCCCAT TGTAGGCAGC ACGGATTATG GAAAGGATGA AGACTCTGCT 
GAGGCTCTCC TGAAGAAGCA TGAAGCTTTG ATGTCTGATC TTTCTGCTTA CGGCAGTAGC 
ATACAGGCAT TAAGGGAACA GGCCCAGTCC TGCAGGCAAC AAGTTGCTCC CACTGATGAT 
GAAACTGGAA AAGAGCTTGT GCTAGCACTC TATGATTACC AAGAGAAGAG TCCTCGGGAG 
GTGACTATGA AGAAAGGAGA TATTCTAACC CTGCTCAACA GCACCAACAA GGACTGGTGG 
AAGGTTGAAG TTAACGATCG TCAGGGCTTT GTACCAGCTG CCTATGTGAA AAAACTAGAT 
CCTGCCCAGT CTGCATCCCG AGAGAATCTT CTAGAAGAGC AAGGCAGCAT AGCATTGCGA 
CAGGAGCAAA TTGACAACCA GACTCTCATT ACTAAGGAAG TCGGCAGTGT ATCTCTGCGT 
ATGAAACAGG TCGAAGAACT GTATCATTCC CTTCTTGAGC TGGGAGAAAA ACGTAAAGGC 
ATGCTAGAAA AAAGCTGCAA GAAGTTTATG CTTTTCCGTG AGGCTAATGA GCTTCAACAG 
TGGATCAATG AGAAGGAAGC TGCGCTCACT AATGAGGAAG TGGGTGCTGA TTTGGAGCAG 
GTGGAGGTGC TACAGAAGAA GTTTGATGAT TTTCAGAAGG ATTTAAAAGC TAATGAGTCA 
CGACTGAAGG ACATAAACAA GGTTGCAAAT GACCTGGAGT CTGAAGGGCT GATGGCAGAG 
GAAGTGCAAG CAGTAGAGCA CCAGGAAGTC TATGGAATGA TGCCCAGAGA TGAAACTGAT 
TCTAAGACAG CCTCTCCTTG GAAGTCTGCA CGTATGATGG TACACACAGT GGCAACGTTT 
AACTCAATCA AGGAACTGAA TGAACGCTGG AGATCCCTGC AGCAGTTGGC AGAGGAGCGA 
AGCCAGCTGT TGGGCAGCGC TCATGAAGTC CAGAGGTTCC ACAGAGATGC TGATGAAACA 
AAAGAATGGA TAGAAGAGAA GAATCAAGCA TTAAATACCG ACAACTATGG ACATGACTTG 
GCCAGTGTGC AGGCTCTGCA GCGCAAGCAT GAAGGCTTTG AGAGAGACTT GGCAGCTCTT 
GGAGACAAGG TGAATTCTCT TGGTGAAACT GCCCAGCGTC TGATCCAGTC ACATCCAGAA 
TCAGCTGAAG ATCTTCAAGA AAAATGTACT GAGTTGAACC AAGCCTGGAG TAGTCTGGGA 
AAACGTGCTG ACCAACGCAA AGAGAAGCTT GGAGATTCTC ATGACCTGCA GCGTTTCCTC 
AGTGATTTTA GAGACCTTAT GTCTTGGATC AATGGAATCC GGGGTCTGGT CTCCTCAGAT 
GAACTTGCAA AAGATGTGAC TGGAGCTGAA GCTCTGTTGG AAAGGCATCA GGAGCACCGT 
ACTGAAATAG ATGCACGGGC TGGCACTTTT CAGGCATTTG AACAGTTTGG ACAGCAGCTT 
CTGGCTCGTG GACACTATGC CAGCCCAGAG ATTAAGGAGA AACTGGATAT TCTAGATCAA 
GAACGGACAG ACCTAGAGAA GGCCTGGGTC CAGCGCAGAA TGATGCTAGA CCAGTGCTTA 
GAACTACAGC TGTTTCATCG GGATTGTGAA CAAGCTGAAA ACTGGATGGC TGCCCGAGAG 
GCGTTCCTAA ATACAGAAGA CAAAGGAGAC TCCTTAGACA GCGTGGAGGC ACTCATCAAG 
AAGCATGAAG ATTTCGATAA AGCAATCAAT GTCCAGGAAG AGAAAATTGC TGTCTTGCAG 
TCTTTTGCTG ACCAACTGAT TGCTGCTGAT CATTATGCAA AAGGAGTCAT TGCTAACAGA 
CGCAATGAGG TTCTGGACAG GTGGCGTCGT CTGAAGGCTC AGATGATTGA GAAGAGATCT 
AAGCTGGGAG AATCTCAGAC CCTCCAACAG TTCAGTCGTG ATGTTGATGA AATAGAAGCT 
TGGATCAGTG AAAAGCTTCA AACTGCAAGT GATGAGTCAT ATAAGGATCC CACAAACATC 
CAGCTTTCCA AACTGCTGAG CAAGCACCAG AAGCACCAAG CCTTTGAAGC TGAGCTCCAC 
GCCAACGCAG ATCGGATTCG TGGAGTCATT GAAATGGGGA ACTCTCTTAT TGAAAGAGGA 
GCGTGTGCTG GCAGCGAGGA TGCTGTGAAG GCTCGGCTGG CTGCCCTGGC TGACCAATGG 
GAGTTCCTGG TCCAGAAGTC ATCAGAGAAG AGTCAGAAAC TGAAAGAAGC AAATAAACAG 
CAGAATTTCA ATACCGGCAT CAAGGACTTT GATTTCTGGC TTTCAGAGGT GGAAGCTTTG 
TTGGCATCTG AAGACTATGG GAAGGACTTG GCATCAGTGA ACAACCTTCT GAAGAAGCAC 
CAATTACTGG AAGCTGATAT ATCTGCTCAT GAGGATCGGC TGAAGGACCT GAACAGCCAG 
GCTGACAGTT TGATGACCAG CAGTGCTTTC GATACCTCCC AAGTAAAGGA TAAACGTGAA 
ACTATAAATG GGCGCTTCCA GAGAATCAAG AGCATGGCAG CTGCCCGCCG TGCGAAGCTC 
AACGAGTCGC ACCGCTTGCA TCAGTTCTTC CGTGACATGG ATGATGAGGA GTCCTGGATC 
AAAGAGAAGA AACTGTTGGT GAGCTCAGAG GACTATGGCA GAGACCTGAC TGGTGTGCAG 
AACCTGAGGA AGAAACATAA GCGCTTGGAA GCAGAATTAG CTGCCCATGA ACCTGCTATC 
CAGGGTGTTC TAGACACTGG CAAGAAGCTT TCAGATGATA ACACAATTGG GAAGGAGGAG 
ATACAGCAAA GACTGGCTCA GTTTGTGGAC CACTGGAAAG AGTTAAAACA GCTGGCAGCT 
GCTCGGGGGC AGCGTCTGGA GGAGTCCCTG GAGTACCAAC AGTTTGTAGC AAATGTTGAG 
GAAGAGGAGG CATGGATCAA CGAGAAAATG ACATTGGTAG CCAGTGAGGA TTATGGAGAC 
ACACTTGCTG CTATCCAGGG CTTGCTGAAG AAGCATGAAG CATTCGAGAC TGACTTTACT 
GTCCACAAAG ACAGAGTGAA TGATGTTTGC GCTAATGGAG AGGATCTCAT TAAAAAGAAT 
AATCACCATG TGGAGAACAT TACTGCTAAG ATGAAGGGCC TCAAGGGCAA GGTATCAGAT 
CTGGAGAAAG CCGCAGCTCA GAGGAAAGCC AAACTGGATG AGAACTCTGC CTTCCTCCAG 
TTCAACTGGA AAGCAGATGT GGTGGAGTCA TGGATAGGTG AGAAGGAAAA CAGTCTGAAG 
ACAGATGATT ATGGCCGTGA CCTCTCCTCT GTGCAAACAC TACTCACCAA ACAGGAAACC 
TTTGATGCTG GACTTCAGGC TTTCCAGCAG GAGGGAATTG CAAACATCAC TGCTCTGAAA 
GACCAGCTAC TGGCAGCCAA ACATATCCAA TCAAAGGCCA TTGAGGTTCG TCACGCTTCC 
TTGATGAAAC GCTGGAATCA GCTGCTTGCT AATTCTGCAG CCAGGAAAAA GAAACTCTTG 
GAGGCTCAGG AGCACTTCAG AAAGGTTGAA GATCTCTTCT TGACTTTTGC CAAGAAGGCC 
TCTGCCTTCA ACAGTTGGTT TGAGAATGCT GAGGAGGACC TGACGGATCC CGTGCGCTGC 
AATTCCCTGG AAGAAATCAA AGCACTGCGA GAAGCCCACG ATGCGTTCCG TTCCTCACTT 
AGCTCTGCCC AAGCTGACTT CAACCAGCTG GCAGAGCTTG ATCGGCAGAT CAAGAGCTTC 
CGTGTAGCCT CCAACCCGTA CACTTGGTTT ACTATGGAGG CTCTTGAAGA AACTTGGAGG 
AATCTGCAGA AAATTATCAA GGAGCGTGAA TTGGAGTTGC AGAAGGAGCA GCGGAGGCAG 
GAAGAAAATG ACAAGTTGCG CCAGGAGTTT GCTCAGCATG CTAATGCCTT CCACCAGTGG 
ATTCAGGAGA CAAGGTCCTG CATGGTGGAG GAGTCGGGAA CACTGGAATC CCAGCTGGAA 
GCTACTAAAC GCAAGCACCA GGAGATCCGG GCTATGAGGA GCCAGCTGAA GAAGATTGAG 
GACCTTGGAG CAGCCATGGA AGAGGCACTT ATCTTGGACA ACAAATACAC AGAGCACAGC 
ACCGTGGGGC TGGCGCAGCA GTGGGACCAG CTTGACCAGC TGGGAATGAG AATGCAGCAC 
AATCTGGAAC AGCAGATTCA AGCTCGAAAC ACAACTGGAG TCACAGAGGA GGCCCTGAAG 
GAGTTCAGCA TGATGTTCAA GCACTTTGAC AAGGACAAAT CTGGACGTCT TAATCACCAG 
GAGTTTAAGT CTTGCTTGCG CTCCCTTGGC TATGACCTGC CCATGGTTGA GGAAGGAGAG 
CCTGACCCCG AATTTGAGTC TATTCTTGAC ACTGTTGATC CCAACAGGGA TGGCCACGTC 
TCGCTGCAGG AGTACATGGC GTTCATGATC AGCAGGGAAA CAGAGAACGT GAAATCCAGC 
GAGGAGATTG AGAGTGCTTT CCGTGCCCTC AGCTCGGAGG GGAAGCCTTA CGTGACCAAG 
GAGGAGCTCT ACCAGAACCT GACCCGGGAG CAGGCCGACT ACTGCATTTC TCACATGAAG 
CCCTACATGG ATGGCAAGGG CAGAGAACTT CCTTCTGCCT ACGACTACAT AGAATTTACA 
CGTTCACTCT TTGTGAATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_015279289.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015134775.1 Gallus gallus spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X8, mRNA.

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CloneID	OGa43315
Clone ID Related Accession (Same CDS sequence)	XM_015279289.1 , XM_015279289.2
Accession Version	XM_015279289.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7497bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCAA GTGGGGTAAA AGTGTTGGAA ACAGCAGAAG ATATCCAAGA ACGGCGTCAG 
CAGGTTTTGG ACCGTTACCA CAGGTTCAAG GAGCTGTCTT CTCTGAGGCG CCAAAAACTC 
GAAGATTCCT ATCGGTTCCA GTTCTTCCAG CGCGATGCAG ATGAGCTGGA AAAATGGATC 
CAAGAGAAAC TCCAGATAGC ATCTGATGAA AATTACAAAG ACCCAAGCAA TTTACAGGGG 
AAGCTGCAGA AGCACCAGGC CTTTGAAGCT GAGGTGCAGG CCAATTCAGG GGCTATCGTT 
AAGCTGGATG AGACTGGAAA TCAGATGATC AATGAAGGCC ATTTTGCATC TGAAACCATA 
AGAACTCGTC TACAGGAGCT GCACCGACTA TGGGAGTTAC TGCTGGAAAA AATGAGAGAG 
AAGGGAGTGA AGCTGTTGCA AGCACAGAAG CTGGTGCAAT TCTTACGGGA ATGTGAAGAT 
GTCATGGACT GGATCAATGA CAAGGAAGCA ATAGTGACCT CAGAAGAGCT TGGACAGGAC 
TTAGAGCATG TTGAGGTTTT GCAGAAGAAA TTTGAAGAGT TCCAAACAGA TCTGGCAGCT 
CATGAGGAGA GAGTGAATGA AGTGAACCAG TTCGCTGGCA AACTTATCCA GGAACAGCAC 
CCTGAGGAGG AACTTATAAA GTCCAAACAG GATGAAGTAA ATGCAAGCTG GCAGCGTCTT 
AAGGGGCTTG CCCAACAGAG GCAAGGAAAA CTCTTTGGGG CAGCTGAAGT TCAGCGCTTC 
AACAGGGATG TGGATGAAAC TATCAGCTGG ATTAAGGAGA AAGGGCAGTT GATGGCCTCA 
GATGACTTTG GCAGAGACTT GGCCAGCGTG CAAGCTTTAC TACGTAAGCA TGAAGGCCTG 
GAAAGAGATC TTGCAGCTTT GGAGGATAAG GTTAAGGCCC TCTGTGCAGA AGCTGACCGT 
TTGCAGCAGT CTCACCCTAT AAATGCTTCT CAAATTCAAG TGAAACGGGA GGAACTGATT 
GCCAACTGGG AGCAGATCCG AACTCTGGCA GCAGAGAGGC ATGCTCGCCT TAATGACTCC 
TACAGGTTGC AGCGCTTTCT TGCAGATTTC CGGGACCTCA CTAGCTGGGT AACTGAGATG 
AAGGCTCTGA TAAATGCTGA TGAGCTTGCC AATGATGTGG CTGGAGCAGA AGCCCTTCTA 
GATAGACATC AGGAACATAA GGGAGAAATT GATGCTCATG AGGATAGCTT CAGATCTGCT 
GATGAGTCTG GGCAGGCTTT GCTTGCAGCT GGGCACTATG CTTCTGATGA AGTTAAAGAA 
AAGCTGACTA TCCTCTCAGA TGAAAGATCA GCCTTGCTGG AACTATGGGA GCTTCGCAGG 
CAACAGTATG AGCAGTGCAT GGATCTGCAG CTTTTCTACA GAGATACTGA ACAAGTTGAC 
AACTGGATGA GCAAACAAGA AGCTTTTCTG CTGAACGAAG ACCTTGGTGA TTCTCTGGAT 
AGCGTGGAGG CTCTTCTAAA GAAGCATGAA GATTTTGAGA AATCCCTAAG TGCTCAAGAG 
GAGAAGATCA CAGCACTGGA TGAGTTTGCT ACTAAACTGA TTCAAAATAA CCATTATGCC 
ATGGATGACG TTGCTACGCG CAGAGATGCT CTTCTGAGTC GCCGAAATGC TCTTCATGAA 
AGAGCCATGA AGCGCCGTGC CCAACTGGCA GACTCTTTCC ATCTCCAGCA GTTTTTCAGG 
GACTCTGATG AACTGAAGAG TTGGGTTAAT GAAAAAATGA AAACTGCTAC CGATGAGGCT 
TACAAGGATC CATCCAACTT GCAAGGTAAA GTTCAGAAGC ATCAGGCTTT TGAAGCAGAG 
CTTTCTGCTA ATCAGAGTCG TATTGATGCA CTGGAGAAAG CTGGCCAGAA GTTGATTGAT 
GTCAATCACT ATGCATCTGA TGAGGTGGCA GCTCGCATGA ATGAAGTCAT CAGCTTGTGG 
AAGAAACTTC TGGAAGCCAC CGAGCTCAAA GGTATAAAGC TGCGTGAAGC CAATCAACAG 
CAGCAATTTA ATCGCAATGT AGAAGACATT GAGTTGTGGC TGTATGAAGT GGAGGGTCAC 
TTGGCTTCTG ATGATTATGG AAAAGATCTT ACCAGCGTTC AGAATCTTCA GAAGAAACAT 
GCCCTGCTAG AGGCAGATGT TGCTGCCCAT CAGGATCGGA TAGATGGCAT TACCATCCAG 
GCACGCCAGT TCCAAGATGC TGGGCACTTT GATGCTGACA ATATCAAGAA GAAACAAGAA 
GCTTTAGTAG CTCGTTATGA AGCTCTGAAG GATCCTATGG TGGCTCGCAA GCAGAAACTT 
GCAGATTCTC TTCGCCTGCA GCAGCTTTTC CGTGACATCG AGGATGAAGA GACCTGGATC 
AGGGAAAAGG AACCTATTGC AGCCTCAACA AACCGAGGCA AGGACTTAAT TGGTGTCCAG 
AATCTGCTAA AGAAGCACCA GGCTTTGCAG GCAGAAATTG CAGGCCATGA GCCTCGCATT 
AAAGCAGTCA CACAAAAAGG AAATGCGATG GTGGAAGAGG GACATTTTGC TGCTGAAGAT 
GTGAAGATCA AATTGAATGA ACTAAACCAA AAATGGGACT CTCTGAAAGC CAAGGCATCT 
CAACGGCGAC AGGATCTAGA GGATTCTCTG CAAGCTCAGC AGTATTTTGC TGATGCTAAT 
GAGGCAGAAT CATGGATGAG GGAAAAGGAG CCCATTGTAG GCAGCACGGA TTATGGAAAG 
GATGAAGACT CTGCTGAGGC TCTCCTGAAG AAGCATGAAG CTTTGATGTC TGATCTTTCT 
GCTTACGGCA GTAGCATACA GGCATTAAGG GAACAGGCCC AGTCCTGCAG GCAACAAGTT 
GCTCCCACTG ATGATGAAAC TGGAAAAGAG CTTGTGCTAG CACTCTATGA TTACCAAGAG 
AAGAGTCCTC GGGAGGTGAC TATGAAGAAA GGAGATATTC TAACCCTGCT CAACAGCACC 
AACAAGGACT GGTGGAAGGT TGAAGTTAAC GATCGTCAGG GCTTTGTACC AGCTGCCTAT 
GTGAAAAAAC TAGATCCTGC CCAGTCTGCA TCCCGAGAGA ATCTTCTAGA AGAGCAAGGC 
AGCATAGCAT TGCGACAGGA GCAAATTGAC AACCAGACTC TCATTACTAA GGAAGTCGGC 
AGTGTATCTC TGCGTATGAA ACAGGTCGAA GAACTGTATC ATTCCCTTCT TGAGCTGGGA 
GAAAAACGTA AAGGCATGCT AGAAAAAAGC TGCAAGAAGT TTATGCTTTT CCGTGAGGCT 
AATGAGCTTC AACAGTGGAT CAATGAGAAG GAAGCTGCGC TCACTAATGA GGAAGTGGGT 
GCTGATTTGG AGCAGGTGGA GGTGCTACAG AAGAAGTTTG ATGATTTTCA GAAGGATTTA 
AAAGCTAATG AGTCACGACT GAAGGACATA AACAAGGTTG CAAATGACCT GGAGTCTGAA 
GGGCTGATGG CAGAGGAAGT GCAAGCAGTA GAGCACCAGG AAGTCTATGG AATGATGCCC 
AGAGATGAAA CTGATTCTAA GACAGCCTCT CCTTGGAAGT CTGCACGTAT GATGGTACAC 
ACAGTGGCAA CGTTTAACTC AATCAAGGAA CTGAATGAAC GCTGGAGATC CCTGCAGCAG 
TTGGCAGAGG AGCGAAGCCA GCTGTTGGGC AGCGCTCATG AAGTCCAGAG GTTCCACAGA 
GATGCTGATG AAACAAAAGA ATGGATAGAA GAGAAGAATC AAGCATTAAA TACCGACAAC 
TATGGACATG ACTTGGCCAG TGTGCAGGCT CTGCAGCGCA AGCATGAAGG CTTTGAGAGA 
GACTTGGCAG CTCTTGGAGA CAAGGTGAAT TCTCTTGGTG AAACTGCCCA GCGTCTGATC 
CAGTCACATC CAGAATCAGC TGAAGATCTT CAAGAAAAAT GTACTGAGTT GAACCAAGCC 
TGGAGTAGTC TGGGAAAACG TGCTGACCAA CGCAAAGAGA AGCTTGGAGA TTCTCATGAC 
CTGCAGCGTT TCCTCAGTGA TTTTAGAGAC CTTATGTCTT GGATCAATGG AATCCGGGGT 
CTGGTCTCCT CAGATGAACT TGCAAAAGAT GTGACTGGAG CTGAAGCTCT GTTGGAAAGG 
CATCAGGAGC ACCGTACTGA AATAGATGCA CGGGCTGGCA CTTTTCAGGC ATTTGAACAG 
TTTGGACAGC AGCTTCTGGC TCGTGGACAC TATGCCAGCC CAGAGATTAA GGAGAAACTG 
GATATTCTAG ATCAAGAACG GACAGACCTA GAGAAGGCCT GGGTCCAGCG CAGAATGATG 
CTAGACCAGT GCTTAGAACT ACAGCTGTTT CATCGGGATT GTGAACAAGC TGAAAACTGG 
ATGGCTGCCC GAGAGGCGTT CCTAAATACA GAAGACAAAG GAGACTCCTT AGACAGCGTG 
GAGGCACTCA TCAAGAAGCA TGAAGATTTC GATAAAGCAA TCAATGTCCA GGAAGAGAAA 
ATTGCTGTCT TGCAGTCTTT TGCTGACCAA CTGATTGCTG CTGATCATTA TGCAAAAGGA 
GTCATTGCTA ACAGACGCAA TGAGGTTCTG GACAGGTGGC GTCGTCTGAA GGCTCAGATG 
ATTGAGAAGA GATCTAAGCT GGGAGAATCT CAGACCCTCC AACAGTTCAG TCGTGATGTT 
GATGAAATAG AAGCTTGGAT CAGTGAAAAG CTTCAAACTG CAAGTGATGA GTCATATAAG 
GATCCCACAA ACATCCAGCT TTCCAAACTG CTGAGCAAGC ACCAGAAGCA CCAAGCCTTT 
GAAGCTGAGC TCCACGCCAA CGCAGATCGG ATTCGTGGAG TCATTGAAAT GGGGAACTCT 
CTTATTGAAA GAGGAGCGTG TGCTGGCAGC GAGGATGCTG TGAAGGCTCG GCTGGCTGCC 
CTGGCTGACC AATGGGAGTT CCTGGTCCAG AAGTCATCAG AGAAGAGTCA GAAACTGAAA 
GAAGCAAATA AACAGCAGAA TTTCAATACC GGCATCAAGG ACTTTGATTT CTGGCTTTCA 
GAGGTGGAAG CTTTGTTGGC ATCTGAAGAC TATGGGAAGG ACTTGGCATC AGTGAACAAC 
CTTCTGAAGA AGCACCAATT ACTGGAAGCT GATATATCTG CTCATGAGGA TCGGCTGAAG 
GACCTGAACA GCCAGGCTGA CAGTTTGATG ACCAGCAGTG CTTTCGATAC CTCCCAAGTA 
AAGGATAAAC GTGAAACTAT AAATGGGCGC TTCCAGAGAA TCAAGAGCAT GGCAGCTGCC 
CGCCGTGCGA AGCTCAACGA GTCGCACCGC TTGCATCAGT TCTTCCGTGA CATGGATGAT 
GAGGAGTCCT GGATCAAAGA GAAGAAACTG TTGGTGAGCT CAGAGGACTA TGGCAGAGAC 
CTGACTGGTG TGCAGAACCT GAGGAAGAAA CATAAGCGCT TGGAAGCAGA ATTAGCTGCC 
CATGAACCTG CTATCCAGGG TGTTCTAGAC ACTGGCAAGA AGCTTTCAGA TGATAACACA 
ATTGGGAAGG AGGAGATACA GCAAAGACTG GCTCAGTTTG TGGACCACTG GAAAGAGTTA 
AAACAGCTGG CAGCTGCTCG GGGGCAGCGT CTGGAGGAGT CCCTGGAGTA CCAACAGTTT 
GTAGCAAATG TTGAGGAAGA GGAGGCATGG ATCAACGAGA AAATGACATT GGTAGCCAGT 
GAGGATTATG GAGACACACT TGCTGCTATC CAGGGCTTGC TGAAGAAGCA TGAAGCATTC 
GAGACTGACT TTACTGTCCA CAAAGACAGA GTGAATGATG TTTGCGCTAA TGGAGAGGAT 
CTCATTAAAA AGAATAATCA CCATGTGGAG AACATTACTG CTAAGATGAA GGGCCTCAAG 
GGCAAGGTAT CAGATCTGGA GAAAGCCGCA GCTCAGAGGA AAGCCAAACT GGATGAGAAC 
TCTGCCTTCC TCCAGTTCAA CTGGAAAGCA GATGTGGTGG AGTCATGGAT AGGTGAGAAG 
GAAAACAGTC TGAAGACAGA TGATTATGGC CGTGACCTCT CCTCTGTGCA AACACTACTC 
ACCAAACAGG AAACCTTTGA TGCTGGACTT CAGGCTTTCC AGCAGGAGGG AATTGCAAAC 
ATCACTGCTC TGAAAGACCA GCTACTGGCA GCCAAACATA TCCAATCAAA GGCCATTGAG 
GTTCGTCACG CTTCCTTGAT GAAACGCTGG AATCAGCTGC TTGCTAATTC TGCAGCCAGG 
AAAAAGAAAC TCTTGGAGGC TCAGGAGCAC TTCAGAAAGG TTGAAGATCT CTTCTTGACT 
TTTGCCAAGA AGGCCTCTGC CTTCAACAGT TGGTTTGAGA ATGCTGAGGA GGACCTGACG 
GATCCCGTGC GCTGCAATTC CCTGGAAGAA ATCAAAGCAC TGCGAGAAGC CCACGATGCG 
TTCCGTTCCT CACTTAGCTC TGCCCAAGCT GACTTCAACC AGCTGGCAGA GCTTGATCGG 
CAGATCAAGA GCTTCCGTGT AGCCTCCAAC CCGTACACTT GGTTTACTAT GGAGGCTCTT 
GAAGAAACTT GGAGGAATCT GCAGAAAATT ATCAAGGAGC GTGAATTGGA GTTGCAGAAG 
GAGCAGCGGA GGCAGGAAGA AAATGACAAG TTGCGCCAGG AGTTTGCTCA GCATGCTAAT 
GCCTTCCACC AGTGGATTCA GGAGACAAGG ACTTACCTGC TAGATGGCAT AGCCTATCGT 
CGTGTCATTC GTGTCTATCA GTATGAAGTT GGGGATGATC TATCTGGAAG GTCCTGCATG 
GTGGAGGAGT CGGGAACACT GGAATCCCAG CTGGAAGCTA CTAAACGCAA GCACCAGGAG 
ATCCGGGCTA TGAGGAGCCA GCTGAAGAAG ATTGAGGACC TTGGAGCAGC CATGGAAGAG 
GCACTTATCT TGGACAACAA ATACACAGAG CACAGCACCG TGGGGCTGGC GCAGCAGTGG 
GACCAGCTTG ACCAGCTGGG AATGAGAATG CAGCACAATC TGGAACAGCA GATTCAAGCT 
CGAAACACAA CTGGAGTCAC AGAGGAGGCC CTGAAGGAGT TCAGCATGAT GTTCAAGCAC 
TTTGACAAGG ACAAATCTGG ACGTCTTAAT CACCAGGAGT TTAAGTCTTG CTTGCGCTCC 
CTTGGCTATG ACCTGCCCAT GGTTGAGGAA GGAGAGCCTG ACCCCGAATT TGAGTCTATT 
CTTGACACTG TTGATCCCAA CAGGGATGGC CACGTCTCGC TGCAGGAGTA CATGGCGTTC 
ATGATCAGCA GGGAAACAGA GAACGTGAAA TCCAGCGAGG AGATTGAGAG TGCTTTCCGT 
GCCCTCAGCT CGGAGGGGAA GCCTTACGTG ACCAAGGAGG AGCTCTACCA GAACCTGACC 
CGGGAGCAGG CCGACTACTG CATTTCTCAC ATGAAGCCCT ACATGGATGG CAAGGGCAGA 
GAACTTCCTT CTGCCTACGA CTACATAGAA TTTACACGTT CACTCTTTGT GAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015134775.1
CDS	110..7606
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSSLRRQKLEDSYRFQFFQRDADELEKWIQEKLQIASDENYKDPSNLQGKLQKHQAFEAEVQANSGAIVKLDETGNQMINEGHFASETIRTRLQELHRLWELLLEKMREKGVKLLQAQKLVQFLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDLAAHEERVNEVNQFAGKLIQEQHPEEELIKSKQDEVNASWQRLKGLAQQRQGKLFGAAEVQRFNRDVDETISWIKEKGQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPINASQIQVKREELIANWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFRSADESGQALLAAGHYASDEVKEKLTILSDERSALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMDDVATRRDALLSRRNALHERAMKRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYASDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTSVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDADNIKKKQEALVARYEALKDPMVARKQKLADSLRLQQLFRDIEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKIKLNELNQKWDSLKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPTDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTLITKEVGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTNEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVANDLESEGLMAEEVQAVEHQEVYGMMPRDETDSKTASPWKSARMMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAQRLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKEKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLARGHYASPEIKEKLDILDQERTDLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAVLQSFADQLIAADHYAKGVIANRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIEMGNSLIERGACAGSEDAVKARLAALADQWEFLVQKSSEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRETINGRFQRIKSMAAARRAKLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVDHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCANGEDLIKKNNHHVENITAKMKGLKGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHIQSKAIEVRHASLMKRWNQLLANSAARKKKLLEAQEHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGIAYRRVIRVYQYEVGDDLSGRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFESILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCISHMKPYMDGKGRELPSAYDYIEFTRSLFVN

Target ORF information:

RefSeq Version	XM_015279289.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279289.1

ORF Insert Sequence:

ATGGATCCAA GTGGGGTAAA AGTGTTGGAA ACAGCAGAAG ATATCCAAGA ACGGCGTCAG 
CAGGTTTTGG ACCGTTACCA CAGGTTCAAG GAGCTGTCTT CTCTGAGGCG CCAAAAACTC 
GAAGATTCCT ATCGGTTCCA GTTCTTCCAG CGCGATGCAG ATGAGCTGGA AAAATGGATC 
CAAGAGAAAC TCCAGATAGC ATCTGATGAA AATTACAAAG ACCCAAGCAA TTTACAGGGG 
AAGCTGCAGA AGCACCAGGC CTTTGAAGCT GAGGTGCAGG CCAATTCAGG GGCTATCGTT 
AAGCTGGATG AGACTGGAAA TCAGATGATC AATGAAGGCC ATTTTGCATC TGAAACCATA 
AGAACTCGTC TACAGGAGCT GCACCGACTA TGGGAGTTAC TGCTGGAAAA AATGAGAGAG 
AAGGGAGTGA AGCTGTTGCA AGCACAGAAG CTGGTGCAAT TCTTACGGGA ATGTGAAGAT 
GTCATGGACT GGATCAATGA CAAGGAAGCA ATAGTGACCT CAGAAGAGCT TGGACAGGAC 
TTAGAGCATG TTGAGGTTTT GCAGAAGAAA TTTGAAGAGT TCCAAACAGA TCTGGCAGCT 
CATGAGGAGA GAGTGAATGA AGTGAACCAG TTCGCTGGCA AACTTATCCA GGAACAGCAC 
CCTGAGGAGG AACTTATAAA GTCCAAACAG GATGAAGTAA ATGCAAGCTG GCAGCGTCTT 
AAGGGGCTTG CCCAACAGAG GCAAGGAAAA CTCTTTGGGG CAGCTGAAGT TCAGCGCTTC 
AACAGGGATG TGGATGAAAC TATCAGCTGG ATTAAGGAGA AAGGGCAGTT GATGGCCTCA 
GATGACTTTG GCAGAGACTT GGCCAGCGTG CAAGCTTTAC TACGTAAGCA TGAAGGCCTG 
GAAAGAGATC TTGCAGCTTT GGAGGATAAG GTTAAGGCCC TCTGTGCAGA AGCTGACCGT 
TTGCAGCAGT CTCACCCTAT AAATGCTTCT CAAATTCAAG TGAAACGGGA GGAACTGATT 
GCCAACTGGG AGCAGATCCG AACTCTGGCA GCAGAGAGGC ATGCTCGCCT TAATGACTCC 
TACAGGTTGC AGCGCTTTCT TGCAGATTTC CGGGACCTCA CTAGCTGGGT AACTGAGATG 
AAGGCTCTGA TAAATGCTGA TGAGCTTGCC AATGATGTGG CTGGAGCAGA AGCCCTTCTA 
GATAGACATC AGGAACATAA GGGAGAAATT GATGCTCATG AGGATAGCTT CAGATCTGCT 
GATGAGTCTG GGCAGGCTTT GCTTGCAGCT GGGCACTATG CTTCTGATGA AGTTAAAGAA 
AAGCTGACTA TCCTCTCAGA TGAAAGATCA GCCTTGCTGG AACTATGGGA GCTTCGCAGG 
CAACAGTATG AGCAGTGCAT GGATCTGCAG CTTTTCTACA GAGATACTGA ACAAGTTGAC 
AACTGGATGA GCAAACAAGA AGCTTTTCTG CTGAACGAAG ACCTTGGTGA TTCTCTGGAT 
AGCGTGGAGG CTCTTCTAAA GAAGCATGAA GATTTTGAGA AATCCCTAAG TGCTCAAGAG 
GAGAAGATCA CAGCACTGGA TGAGTTTGCT ACTAAACTGA TTCAAAATAA CCATTATGCC 
ATGGATGACG TTGCTACGCG CAGAGATGCT CTTCTGAGTC GCCGAAATGC TCTTCATGAA 
AGAGCCATGA AGCGCCGTGC CCAACTGGCA GACTCTTTCC ATCTCCAGCA GTTTTTCAGG 
GACTCTGATG AACTGAAGAG TTGGGTTAAT GAAAAAATGA AAACTGCTAC CGATGAGGCT 
TACAAGGATC CATCCAACTT GCAAGGTAAA GTTCAGAAGC ATCAGGCTTT TGAAGCAGAG 
CTTTCTGCTA ATCAGAGTCG TATTGATGCA CTGGAGAAAG CTGGCCAGAA GTTGATTGAT 
GTCAATCACT ATGCATCTGA TGAGGTGGCA GCTCGCATGA ATGAAGTCAT CAGCTTGTGG 
AAGAAACTTC TGGAAGCCAC CGAGCTCAAA GGTATAAAGC TGCGTGAAGC CAATCAACAG 
CAGCAATTTA ATCGCAATGT AGAAGACATT GAGTTGTGGC TGTATGAAGT GGAGGGTCAC 
TTGGCTTCTG ATGATTATGG AAAAGATCTT ACCAGCGTTC AGAATCTTCA GAAGAAACAT 
GCCCTGCTAG AGGCAGATGT TGCTGCCCAT CAGGATCGGA TAGATGGCAT TACCATCCAG 
GCACGCCAGT TCCAAGATGC TGGGCACTTT GATGCTGACA ATATCAAGAA GAAACAAGAA 
GCTTTAGTAG CTCGTTATGA AGCTCTGAAG GATCCTATGG TGGCTCGCAA GCAGAAACTT 
GCAGATTCTC TTCGCCTGCA GCAGCTTTTC CGTGACATCG AGGATGAAGA GACCTGGATC 
AGGGAAAAGG AACCTATTGC AGCCTCAACA AACCGAGGCA AGGACTTAAT TGGTGTCCAG 
AATCTGCTAA AGAAGCACCA GGCTTTGCAG GCAGAAATTG CAGGCCATGA GCCTCGCATT 
AAAGCAGTCA CACAAAAAGG AAATGCGATG GTGGAAGAGG GACATTTTGC TGCTGAAGAT 
GTGAAGATCA AATTGAATGA ACTAAACCAA AAATGGGACT CTCTGAAAGC CAAGGCATCT 
CAACGGCGAC AGGATCTAGA GGATTCTCTG CAAGCTCAGC AGTATTTTGC TGATGCTAAT 
GAGGCAGAAT CATGGATGAG GGAAAAGGAG CCCATTGTAG GCAGCACGGA TTATGGAAAG 
GATGAAGACT CTGCTGAGGC TCTCCTGAAG AAGCATGAAG CTTTGATGTC TGATCTTTCT 
GCTTACGGCA GTAGCATACA GGCATTAAGG GAACAGGCCC AGTCCTGCAG GCAACAAGTT 
GCTCCCACTG ATGATGAAAC TGGAAAAGAG CTTGTGCTAG CACTCTATGA TTACCAAGAG 
AAGAGTCCTC GGGAGGTGAC TATGAAGAAA GGAGATATTC TAACCCTGCT CAACAGCACC 
AACAAGGACT GGTGGAAGGT TGAAGTTAAC GATCGTCAGG GCTTTGTACC AGCTGCCTAT 
GTGAAAAAAC TAGATCCTGC CCAGTCTGCA TCCCGAGAGA ATCTTCTAGA AGAGCAAGGC 
AGCATAGCAT TGCGACAGGA GCAAATTGAC AACCAGACTC TCATTACTAA GGAAGTCGGC 
AGTGTATCTC TGCGTATGAA ACAGGTCGAA GAACTGTATC ATTCCCTTCT TGAGCTGGGA 
GAAAAACGTA AAGGCATGCT AGAAAAAAGC TGCAAGAAGT TTATGCTTTT CCGTGAGGCT 
AATGAGCTTC AACAGTGGAT CAATGAGAAG GAAGCTGCGC TCACTAATGA GGAAGTGGGT 
GCTGATTTGG AGCAGGTGGA GGTGCTACAG AAGAAGTTTG ATGATTTTCA GAAGGATTTA 
AAAGCTAATG AGTCACGACT GAAGGACATA AACAAGGTTG CAAATGACCT GGAGTCTGAA 
GGGCTGATGG CAGAGGAAGT GCAAGCAGTA GAGCACCAGG AAGTCTATGG AATGATGCCC 
AGAGATGAAA CTGATTCTAA GACAGCCTCT CCTTGGAAGT CTGCACGTAT GATGGTACAC 
ACAGTGGCAA CGTTTAACTC AATCAAGGAA CTGAATGAAC GCTGGAGATC CCTGCAGCAG 
TTGGCAGAGG AGCGAAGCCA GCTGTTGGGC AGCGCTCATG AAGTCCAGAG GTTCCACAGA 
GATGCTGATG AAACAAAAGA ATGGATAGAA GAGAAGAATC AAGCATTAAA TACCGACAAC 
TATGGACATG ACTTGGCCAG TGTGCAGGCT CTGCAGCGCA AGCATGAAGG CTTTGAGAGA 
GACTTGGCAG CTCTTGGAGA CAAGGTGAAT TCTCTTGGTG AAACTGCCCA GCGTCTGATC 
CAGTCACATC CAGAATCAGC TGAAGATCTT CAAGAAAAAT GTACTGAGTT GAACCAAGCC 
TGGAGTAGTC TGGGAAAACG TGCTGACCAA CGCAAAGAGA AGCTTGGAGA TTCTCATGAC 
CTGCAGCGTT TCCTCAGTGA TTTTAGAGAC CTTATGTCTT GGATCAATGG AATCCGGGGT 
CTGGTCTCCT CAGATGAACT TGCAAAAGAT GTGACTGGAG CTGAAGCTCT GTTGGAAAGG 
CATCAGGAGC ACCGTACTGA AATAGATGCA CGGGCTGGCA CTTTTCAGGC ATTTGAACAG 
TTTGGACAGC AGCTTCTGGC TCGTGGACAC TATGCCAGCC CAGAGATTAA GGAGAAACTG 
GATATTCTAG ATCAAGAACG GACAGACCTA GAGAAGGCCT GGGTCCAGCG CAGAATGATG 
CTAGACCAGT GCTTAGAACT ACAGCTGTTT CATCGGGATT GTGAACAAGC TGAAAACTGG 
ATGGCTGCCC GAGAGGCGTT CCTAAATACA GAAGACAAAG GAGACTCCTT AGACAGCGTG 
GAGGCACTCA TCAAGAAGCA TGAAGATTTC GATAAAGCAA TCAATGTCCA GGAAGAGAAA 
ATTGCTGTCT TGCAGTCTTT TGCTGACCAA CTGATTGCTG CTGATCATTA TGCAAAAGGA 
GTCATTGCTA ACAGACGCAA TGAGGTTCTG GACAGGTGGC GTCGTCTGAA GGCTCAGATG 
ATTGAGAAGA GATCTAAGCT GGGAGAATCT CAGACCCTCC AACAGTTCAG TCGTGATGTT 
GATGAAATAG AAGCTTGGAT CAGTGAAAAG CTTCAAACTG CAAGTGATGA GTCATATAAG 
GATCCCACAA ACATCCAGCT TTCCAAACTG CTGAGCAAGC ACCAGAAGCA CCAAGCCTTT 
GAAGCTGAGC TCCACGCCAA CGCAGATCGG ATTCGTGGAG TCATTGAAAT GGGGAACTCT 
CTTATTGAAA GAGGAGCGTG TGCTGGCAGC GAGGATGCTG TGAAGGCTCG GCTGGCTGCC 
CTGGCTGACC AATGGGAGTT CCTGGTCCAG AAGTCATCAG AGAAGAGTCA GAAACTGAAA 
GAAGCAAATA AACAGCAGAA TTTCAATACC GGCATCAAGG ACTTTGATTT CTGGCTTTCA 
GAGGTGGAAG CTTTGTTGGC ATCTGAAGAC TATGGGAAGG ACTTGGCATC AGTGAACAAC 
CTTCTGAAGA AGCACCAATT ACTGGAAGCT GATATATCTG CTCATGAGGA TCGGCTGAAG 
GACCTGAACA GCCAGGCTGA CAGTTTGATG ACCAGCAGTG CTTTCGATAC CTCCCAAGTA 
AAGGATAAAC GTGAAACTAT AAATGGGCGC TTCCAGAGAA TCAAGAGCAT GGCAGCTGCC 
CGCCGTGCGA AGCTCAACGA GTCGCACCGC TTGCATCAGT TCTTCCGTGA CATGGATGAT 
GAGGAGTCCT GGATCAAAGA GAAGAAACTG TTGGTGAGCT CAGAGGACTA TGGCAGAGAC 
CTGACTGGTG TGCAGAACCT GAGGAAGAAA CATAAGCGCT TGGAAGCAGA ATTAGCTGCC 
CATGAACCTG CTATCCAGGG TGTTCTAGAC ACTGGCAAGA AGCTTTCAGA TGATAACACA 
ATTGGGAAGG AGGAGATACA GCAAAGACTG GCTCAGTTTG TGGACCACTG GAAAGAGTTA 
AAACAGCTGG CAGCTGCTCG GGGGCAGCGT CTGGAGGAGT CCCTGGAGTA CCAACAGTTT 
GTAGCAAATG TTGAGGAAGA GGAGGCATGG ATCAACGAGA AAATGACATT GGTAGCCAGT 
GAGGATTATG GAGACACACT TGCTGCTATC CAGGGCTTGC TGAAGAAGCA TGAAGCATTC 
GAGACTGACT TTACTGTCCA CAAAGACAGA GTGAATGATG TTTGCGCTAA TGGAGAGGAT 
CTCATTAAAA AGAATAATCA CCATGTGGAG AACATTACTG CTAAGATGAA GGGCCTCAAG 
GGCAAGGTAT CAGATCTGGA GAAAGCCGCA GCTCAGAGGA AAGCCAAACT GGATGAGAAC 
TCTGCCTTCC TCCAGTTCAA CTGGAAAGCA GATGTGGTGG AGTCATGGAT AGGTGAGAAG 
GAAAACAGTC TGAAGACAGA TGATTATGGC CGTGACCTCT CCTCTGTGCA AACACTACTC 
ACCAAACAGG AAACCTTTGA TGCTGGACTT CAGGCTTTCC AGCAGGAGGG AATTGCAAAC 
ATCACTGCTC TGAAAGACCA GCTACTGGCA GCCAAACATA TCCAATCAAA GGCCATTGAG 
GTTCGTCACG CTTCCTTGAT GAAACGCTGG AATCAGCTGC TTGCTAATTC TGCAGCCAGG 
AAAAAGAAAC TCTTGGAGGC TCAGGAGCAC TTCAGAAAGG TTGAAGATCT CTTCTTGACT 
TTTGCCAAGA AGGCCTCTGC CTTCAACAGT TGGTTTGAGA ATGCTGAGGA GGACCTGACG 
GATCCCGTGC GCTGCAATTC CCTGGAAGAA ATCAAAGCAC TGCGAGAAGC CCACGATGCG 
TTCCGTTCCT CACTTAGCTC TGCCCAAGCT GACTTCAACC AGCTGGCAGA GCTTGATCGG 
CAGATCAAGA GCTTCCGTGT AGCCTCCAAC CCGTACACTT GGTTTACTAT GGAGGCTCTT 
GAAGAAACTT GGAGGAATCT GCAGAAAATT ATCAAGGAGC GTGAATTGGA GTTGCAGAAG 
GAGCAGCGGA GGCAGGAAGA AAATGACAAG TTGCGCCAGG AGTTTGCTCA GCATGCTAAT 
GCCTTCCACC AGTGGATTCA GGAGACAAGG ACTTACCTGC TAGATGGCAT AGCCTATCGT 
CGTGTCATTC GTGTCTATCA GTATGAAGTT GGGGATGATC TATCTGGAAG GTCCTGCATG 
GTGGAGGAGT CGGGAACACT GGAATCCCAG CTGGAAGCTA CTAAACGCAA GCACCAGGAG 
ATCCGGGCTA TGAGGAGCCA GCTGAAGAAG ATTGAGGACC TTGGAGCAGC CATGGAAGAG 
GCACTTATCT TGGACAACAA ATACACAGAG CACAGCACCG TGGGGCTGGC GCAGCAGTGG 
GACCAGCTTG ACCAGCTGGG AATGAGAATG CAGCACAATC TGGAACAGCA GATTCAAGCT 
CGAAACACAA CTGGAGTCAC AGAGGAGGCC CTGAAGGAGT TCAGCATGAT GTTCAAGCAC 
TTTGACAAGG ACAAATCTGG ACGTCTTAAT CACCAGGAGT TTAAGTCTTG CTTGCGCTCC 
CTTGGCTATG ACCTGCCCAT GGTTGAGGAA GGAGAGCCTG ACCCCGAATT TGAGTCTATT 
CTTGACACTG TTGATCCCAA CAGGGATGGC CACGTCTCGC TGCAGGAGTA CATGGCGTTC 
ATGATCAGCA GGGAAACAGA GAACGTGAAA TCCAGCGAGG AGATTGAGAG TGCTTTCCGT 
GCCCTCAGCT CGGAGGGGAA GCCTTACGTG ACCAAGGAGG AGCTCTACCA GAACCTGACC 
CGGGAGCAGG CCGACTACTG CATTTCTCAC ATGAAGCCCT ACATGGATGG CAAGGGCAGA 
GAACTTCCTT CTGCCTACGA CTACATAGAA TTTACACGTT CACTCTTTGT GAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_015279289.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015134775.1 Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X8, mRNA.

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CloneID	OGa43315
Clone ID Related Accession (Same CDS sequence)	XM_015279289.1 , XM_015279289.2
Accession Version	XM_015279289.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7497bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015279289.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCAA GTGGGGTAAA AGTGTTGGAA ACAGCAGAAG ATATCCAAGA ACGGCGTCAG 
CAGGTTTTGG ACCGTTACCA CAGGTTCAAG GAGCTGTCTT CTCTGAGGCG CCAAAAACTC 
GAAGATTCCT ATCGGTTCCA GTTCTTCCAG CGCGATGCAG ATGAGCTGGA AAAATGGATC 
CAAGAGAAAC TCCAGATAGC ATCTGATGAA AATTACAAAG ACCCAAGCAA TTTACAGGGG 
AAGCTGCAGA AGCACCAGGC CTTTGAAGCT GAGGTGCAGG CCAATTCAGG GGCTATCGTT 
AAGCTGGATG AGACTGGAAA TCAGATGATC AATGAAGGCC ATTTTGCATC TGAAACCATA 
AGAACTCGTC TACAGGAGCT GCACCGACTA TGGGAGTTAC TGCTGGAAAA AATGAGAGAG 
AAGGGAGTGA AGCTGTTGCA AGCACAGAAG CTGGTGCAAT TCTTACGGGA ATGTGAAGAT 
GTCATGGACT GGATCAATGA CAAGGAAGCA ATAGTGACCT CAGAAGAGCT TGGACAGGAC 
TTAGAGCATG TTGAGGTTTT GCAGAAGAAA TTTGAAGAGT TCCAAACAGA TCTGGCAGCT 
CATGAGGAGA GAGTGAATGA AGTGAACCAG TTCGCTGGCA AACTTATCCA GGAACAGCAC 
CCTGAGGAGG AACTTATAAA GTCCAAACAG GATGAAGTAA ATGCAAGCTG GCAGCGTCTT 
AAGGGGCTTG CCCAACAGAG GCAAGGAAAA CTCTTTGGGG CAGCTGAAGT TCAGCGCTTC 
AACAGGGATG TGGATGAAAC TATCAGCTGG ATTAAGGAGA AAGGGCAGTT GATGGCCTCA 
GATGACTTTG GCAGAGACTT GGCCAGCGTG CAAGCTTTAC TACGTAAGCA TGAAGGCCTG 
GAAAGAGATC TTGCAGCTTT GGAGGATAAG GTTAAGGCCC TCTGTGCAGA AGCTGACCGT 
TTGCAGCAGT CTCACCCTAT AAATGCTTCT CAAATTCAAG TGAAACGGGA GGAACTGATT 
GCCAACTGGG AGCAGATCCG AACTCTGGCA GCAGAGAGGC ATGCTCGCCT TAATGACTCC 
TACAGGTTGC AGCGCTTTCT TGCAGATTTC CGGGACCTCA CTAGCTGGGT AACTGAGATG 
AAGGCTCTGA TAAATGCTGA TGAGCTTGCC AATGATGTGG CTGGAGCAGA AGCCCTTCTA 
GATAGACATC AGGAACATAA GGGAGAAATT GATGCTCATG AGGATAGCTT CAGATCTGCT 
GATGAGTCTG GGCAGGCTTT GCTTGCAGCT GGGCACTATG CTTCTGATGA AGTTAAAGAA 
AAGCTGACTA TCCTCTCAGA TGAAAGATCA GCCTTGCTGG AACTATGGGA GCTTCGCAGG 
CAACAGTATG AGCAGTGCAT GGATCTGCAG CTTTTCTACA GAGATACTGA ACAAGTTGAC 
AACTGGATGA GCAAACAAGA AGCTTTTCTG CTGAACGAAG ACCTTGGTGA TTCTCTGGAT 
AGCGTGGAGG CTCTTCTAAA GAAGCATGAA GATTTTGAGA AATCCCTAAG TGCTCAAGAG 
GAGAAGATCA CAGCACTGGA TGAGTTTGCT ACTAAACTGA TTCAAAATAA CCATTATGCC 
ATGGATGACG TTGCTACGCG CAGAGATGCT CTTCTGAGTC GCCGAAATGC TCTTCATGAA 
AGAGCCATGA AGCGCCGTGC CCAACTGGCA GACTCTTTCC ATCTCCAGCA GTTTTTCAGG 
GACTCTGATG AACTGAAGAG TTGGGTTAAT GAAAAAATGA AAACTGCTAC CGATGAGGCT 
TACAAGGATC CATCCAACTT GCAAGGTAAA GTTCAGAAGC ATCAGGCTTT TGAAGCAGAG 
CTTTCTGCTA ATCAGAGTCG TATTGATGCA CTGGAGAAAG CTGGCCAGAA GTTGATTGAT 
GTCAATCACT ATGCATCTGA TGAGGTGGCA GCTCGCATGA ATGAAGTCAT CAGCTTGTGG 
AAGAAACTTC TGGAAGCCAC CGAGCTCAAA GGTATAAAGC TGCGTGAAGC CAATCAACAG 
CAGCAATTTA ATCGCAATGT AGAAGACATT GAGTTGTGGC TGTATGAAGT GGAGGGTCAC 
TTGGCTTCTG ATGATTATGG AAAAGATCTT ACCAGCGTTC AGAATCTTCA GAAGAAACAT 
GCCCTGCTAG AGGCAGATGT TGCTGCCCAT CAGGATCGGA TAGATGGCAT TACCATCCAG 
GCACGCCAGT TCCAAGATGC TGGGCACTTT GATGCTGACA ATATCAAGAA GAAACAAGAA 
GCTTTAGTAG CTCGTTATGA AGCTCTGAAG GATCCTATGG TGGCTCGCAA GCAGAAACTT 
GCAGATTCTC TTCGCCTGCA GCAGCTTTTC CGTGACATCG AGGATGAAGA GACCTGGATC 
AGGGAAAAGG AACCTATTGC AGCCTCAACA AACCGAGGCA AGGACTTAAT TGGTGTCCAG 
AATCTGCTAA AGAAGCACCA GGCTTTGCAG GCAGAAATTG CAGGCCATGA GCCTCGCATT 
AAAGCAGTCA CACAAAAAGG AAATGCGATG GTGGAAGAGG GACATTTTGC TGCTGAAGAT 
GTGAAGATCA AATTGAATGA ACTAAACCAA AAATGGGACT CTCTGAAAGC CAAGGCATCT 
CAACGGCGAC AGGATCTAGA GGATTCTCTG CAAGCTCAGC AGTATTTTGC TGATGCTAAT 
GAGGCAGAAT CATGGATGAG GGAAAAGGAG CCCATTGTAG GCAGCACGGA TTATGGAAAG 
GATGAAGACT CTGCTGAGGC TCTCCTGAAG AAGCATGAAG CTTTGATGTC TGATCTTTCT 
GCTTACGGCA GTAGCATACA GGCATTAAGG GAACAGGCCC AGTCCTGCAG GCAACAAGTT 
GCTCCCACTG ATGATGAAAC TGGAAAAGAG CTTGTGCTAG CACTCTATGA TTACCAAGAG 
AAGAGTCCTC GGGAGGTGAC TATGAAGAAA GGAGATATTC TAACCCTGCT CAACAGCACC 
AACAAGGACT GGTGGAAGGT TGAAGTTAAC GATCGTCAGG GCTTTGTACC AGCTGCCTAT 
GTGAAAAAAC TAGATCCTGC CCAGTCTGCA TCCCGAGAGA ATCTTCTAGA AGAGCAAGGC 
AGCATAGCAT TGCGACAGGA GCAAATTGAC AACCAGACTC TCATTACTAA GGAAGTCGGC 
AGTGTATCTC TGCGTATGAA ACAGGTCGAA GAACTGTATC ATTCCCTTCT TGAGCTGGGA 
GAAAAACGTA AAGGCATGCT AGAAAAAAGC TGCAAGAAGT TTATGCTTTT CCGTGAGGCT 
AATGAGCTTC AACAGTGGAT CAATGAGAAG GAAGCTGCGC TCACTAATGA GGAAGTGGGT 
GCTGATTTGG AGCAGGTGGA GGTGCTACAG AAGAAGTTTG ATGATTTTCA GAAGGATTTA 
AAAGCTAATG AGTCACGACT GAAGGACATA AACAAGGTTG CAAATGACCT GGAGTCTGAA 
GGGCTGATGG CAGAGGAAGT GCAAGCAGTA GAGCACCAGG AAGTCTATGG AATGATGCCC 
AGAGATGAAA CTGATTCTAA GACAGCCTCT CCTTGGAAGT CTGCACGTAT GATGGTACAC 
ACAGTGGCAA CGTTTAACTC AATCAAGGAA CTGAATGAAC GCTGGAGATC CCTGCAGCAG 
TTGGCAGAGG AGCGAAGCCA GCTGTTGGGC AGCGCTCATG AAGTCCAGAG GTTCCACAGA 
GATGCTGATG AAACAAAAGA ATGGATAGAA GAGAAGAATC AAGCATTAAA TACCGACAAC 
TATGGACATG ACTTGGCCAG TGTGCAGGCT CTGCAGCGCA AGCATGAAGG CTTTGAGAGA 
GACTTGGCAG CTCTTGGAGA CAAGGTGAAT TCTCTTGGTG AAACTGCCCA GCGTCTGATC 
CAGTCACATC CAGAATCAGC TGAAGATCTT CAAGAAAAAT GTACTGAGTT GAACCAAGCC 
TGGAGTAGTC TGGGAAAACG TGCTGACCAA CGCAAAGAGA AGCTTGGAGA TTCTCATGAC 
CTGCAGCGTT TCCTCAGTGA TTTTAGAGAC CTTATGTCTT GGATCAATGG AATCCGGGGT 
CTGGTCTCCT CAGATGAACT TGCAAAAGAT GTGACTGGAG CTGAAGCTCT GTTGGAAAGG 
CATCAGGAGC ACCGTACTGA AATAGATGCA CGGGCTGGCA CTTTTCAGGC ATTTGAACAG 
TTTGGACAGC AGCTTCTGGC TCGTGGACAC TATGCCAGCC CAGAGATTAA GGAGAAACTG 
GATATTCTAG ATCAAGAACG GACAGACCTA GAGAAGGCCT GGGTCCAGCG CAGAATGATG 
CTAGACCAGT GCTTAGAACT ACAGCTGTTT CATCGGGATT GTGAACAAGC TGAAAACTGG 
ATGGCTGCCC GAGAGGCGTT CCTAAATACA GAAGACAAAG GAGACTCCTT AGACAGCGTG 
GAGGCACTCA TCAAGAAGCA TGAAGATTTC GATAAAGCAA TCAATGTCCA GGAAGAGAAA 
ATTGCTGTCT TGCAGTCTTT TGCTGACCAA CTGATTGCTG CTGATCATTA TGCAAAAGGA 
GTCATTGCTA ACAGACGCAA TGAGGTTCTG GACAGGTGGC GTCGTCTGAA GGCTCAGATG 
ATTGAGAAGA GATCTAAGCT GGGAGAATCT CAGACCCTCC AACAGTTCAG TCGTGATGTT 
GATGAAATAG AAGCTTGGAT CAGTGAAAAG CTTCAAACTG CAAGTGATGA GTCATATAAG 
GATCCCACAA ACATCCAGCT TTCCAAACTG CTGAGCAAGC ACCAGAAGCA CCAAGCCTTT 
GAAGCTGAGC TCCACGCCAA CGCAGATCGG ATTCGTGGAG TCATTGAAAT GGGGAACTCT 
CTTATTGAAA GAGGAGCGTG TGCTGGCAGC GAGGATGCTG TGAAGGCTCG GCTGGCTGCC 
CTGGCTGACC AATGGGAGTT CCTGGTCCAG AAGTCATCAG AGAAGAGTCA GAAACTGAAA 
GAAGCAAATA AACAGCAGAA TTTCAATACC GGCATCAAGG ACTTTGATTT CTGGCTTTCA 
GAGGTGGAAG CTTTGTTGGC ATCTGAAGAC TATGGGAAGG ACTTGGCATC AGTGAACAAC 
CTTCTGAAGA AGCACCAATT ACTGGAAGCT GATATATCTG CTCATGAGGA TCGGCTGAAG 
GACCTGAACA GCCAGGCTGA CAGTTTGATG ACCAGCAGTG CTTTCGATAC CTCCCAAGTA 
AAGGATAAAC GTGAAACTAT AAATGGGCGC TTCCAGAGAA TCAAGAGCAT GGCAGCTGCC 
CGCCGTGCGA AGCTCAACGA GTCGCACCGC TTGCATCAGT TCTTCCGTGA CATGGATGAT 
GAGGAGTCCT GGATCAAAGA GAAGAAACTG TTGGTGAGCT CAGAGGACTA TGGCAGAGAC 
CTGACTGGTG TGCAGAACCT GAGGAAGAAA CATAAGCGCT TGGAAGCAGA ATTAGCTGCC 
CATGAACCTG CTATCCAGGG TGTTCTAGAC ACTGGCAAGA AGCTTTCAGA TGATAACACA 
ATTGGGAAGG AGGAGATACA GCAAAGACTG GCTCAGTTTG TGGACCACTG GAAAGAGTTA 
AAACAGCTGG CAGCTGCTCG GGGGCAGCGT CTGGAGGAGT CCCTGGAGTA CCAACAGTTT 
GTAGCAAATG TTGAGGAAGA GGAGGCATGG ATCAACGAGA AAATGACATT GGTAGCCAGT 
GAGGATTATG GAGACACACT TGCTGCTATC CAGGGCTTGC TGAAGAAGCA TGAAGCATTC 
GAGACTGACT TTACTGTCCA CAAAGACAGA GTGAATGATG TTTGCGCTAA TGGAGAGGAT 
CTCATTAAAA AGAATAATCA CCATGTGGAG AACATTACTG CTAAGATGAA GGGCCTCAAG 
GGCAAGGTAT CAGATCTGGA GAAAGCCGCA GCTCAGAGGA AAGCCAAACT GGATGAGAAC 
TCTGCCTTCC TCCAGTTCAA CTGGAAAGCA GATGTGGTGG AGTCATGGAT AGGTGAGAAG 
GAAAACAGTC TGAAGACAGA TGATTATGGC CGTGACCTCT CCTCTGTGCA AACACTACTC 
ACCAAACAGG AAACCTTTGA TGCTGGACTT CAGGCTTTCC AGCAGGAGGG AATTGCAAAC 
ATCACTGCTC TGAAAGACCA GCTACTGGCA GCCAAACATA TCCAATCAAA GGCCATTGAG 
GTTCGTCACG CTTCCTTGAT GAAACGCTGG AATCAGCTGC TTGCTAATTC TGCAGCCAGG 
AAAAAGAAAC TCTTGGAGGC TCAGGAGCAC TTCAGAAAGG TTGAAGATCT CTTCTTGACT 
TTTGCCAAGA AGGCCTCTGC CTTCAACAGT TGGTTTGAGA ATGCTGAGGA GGACCTGACG 
GATCCCGTGC GCTGCAATTC CCTGGAAGAA ATCAAAGCAC TGCGAGAAGC CCACGATGCG 
TTCCGTTCCT CACTTAGCTC TGCCCAAGCT GACTTCAACC AGCTGGCAGA GCTTGATCGG 
CAGATCAAGA GCTTCCGTGT AGCCTCCAAC CCGTACACTT GGTTTACTAT GGAGGCTCTT 
GAAGAAACTT GGAGGAATCT GCAGAAAATT ATCAAGGAGC GTGAATTGGA GTTGCAGAAG 
GAGCAGCGGA GGCAGGAAGA AAATGACAAG TTGCGCCAGG AGTTTGCTCA GCATGCTAAT 
GCCTTCCACC AGTGGATTCA GGAGACAAGG ACTTACCTGC TAGATGGCAT AGCCTATCGT 
CGTGTCATTC GTGTCTATCA GTATGAAGTT GGGGATGATC TATCTGGAAG GTCCTGCATG 
GTGGAGGAGT CGGGAACACT GGAATCCCAG CTGGAAGCTA CTAAACGCAA GCACCAGGAG 
ATCCGGGCTA TGAGGAGCCA GCTGAAGAAG ATTGAGGACC TTGGAGCAGC CATGGAAGAG 
GCACTTATCT TGGACAACAA ATACACAGAG CACAGCACCG TGGGGCTGGC GCAGCAGTGG 
GACCAGCTTG ACCAGCTGGG AATGAGAATG CAGCACAATC TGGAACAGCA GATTCAAGCT 
CGAAACACAA CTGGAGTCAC AGAGGAGGCC CTGAAGGAGT TCAGCATGAT GTTCAAGCAC 
TTTGACAAGG ACAAATCTGG ACGTCTTAAT CACCAGGAGT TTAAGTCTTG CTTGCGCTCC 
CTTGGCTATG ACCTGCCCAT GGTTGAGGAA GGAGAGCCTG ACCCCGAATT TGAGTCTATT 
CTTGACACTG TTGATCCCAA CAGGGATGGC CACGTCTCGC TGCAGGAGTA CATGGCGTTC 
ATGATCAGCA GGGAAACAGA GAACGTGAAA TCCAGCGAGG AGATTGAGAG TGCTTTCCGT 
GCCCTCAGCT CGGAGGGGAA GCCTTACGTG ACCAAGGAGG AGCTCTACCA GAACCTGACC 
CGGGAGCAGG CCGACTACTG CATTTCTCAC ATGAAGCCCT ACATGGATGG CAAGGGCAGA 
GAACTTCCTT CTGCCTACGA CTACATAGAA TTTACACGTT CACTCTTTGT GAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015134775.1
CDS	159..7655
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSSLRRQKLEDSYRFQFFQRDADELEKWIQEKLQIASDENYKDPSNLQGKLQKHQAFEAEVQANSGAIVKLDETGNQMINEGHFASETIRTRLQELHRLWELLLEKMREKGVKLLQAQKLVQFLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDLAAHEERVNEVNQFAGKLIQEQHPEEELIKSKQDEVNASWQRLKGLAQQRQGKLFGAAEVQRFNRDVDETISWIKEKGQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPINASQIQVKREELIANWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFRSADESGQALLAAGHYASDEVKEKLTILSDERSALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMDDVATRRDALLSRRNALHERAMKRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYASDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTSVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDADNIKKKQEALVARYEALKDPMVARKQKLADSLRLQQLFRDIEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKIKLNELNQKWDSLKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPTDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTLITKEVGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTNEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVANDLESEGLMAEEVQAVEHQEVYGMMPRDETDSKTASPWKSARMMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAQRLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKEKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLARGHYASPEIKEKLDILDQERTDLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAVLQSFADQLIAADHYAKGVIANRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIEMGNSLIERGACAGSEDAVKARLAALADQWEFLVQKSSEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRETINGRFQRIKSMAAARRAKLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVDHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCANGEDLIKKNNHHVENITAKMKGLKGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHIQSKAIEVRHASLMKRWNQLLANSAARKKKLLEAQEHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGIAYRRVIRVYQYEVGDDLSGRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFESILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCISHMKPYMDGKGRELPSAYDYIEFTRSLFVN

Target ORF information:

RefSeq Version	XM_015279289.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279289.2

ORF Insert Sequence:

ATGGATCCAA GTGGGGTAAA AGTGTTGGAA ACAGCAGAAG ATATCCAAGA ACGGCGTCAG 
CAGGTTTTGG ACCGTTACCA CAGGTTCAAG GAGCTGTCTT CTCTGAGGCG CCAAAAACTC 
GAAGATTCCT ATCGGTTCCA GTTCTTCCAG CGCGATGCAG ATGAGCTGGA AAAATGGATC 
CAAGAGAAAC TCCAGATAGC ATCTGATGAA AATTACAAAG ACCCAAGCAA TTTACAGGGG 
AAGCTGCAGA AGCACCAGGC CTTTGAAGCT GAGGTGCAGG CCAATTCAGG GGCTATCGTT 
AAGCTGGATG AGACTGGAAA TCAGATGATC AATGAAGGCC ATTTTGCATC TGAAACCATA 
AGAACTCGTC TACAGGAGCT GCACCGACTA TGGGAGTTAC TGCTGGAAAA AATGAGAGAG 
AAGGGAGTGA AGCTGTTGCA AGCACAGAAG CTGGTGCAAT TCTTACGGGA ATGTGAAGAT 
GTCATGGACT GGATCAATGA CAAGGAAGCA ATAGTGACCT CAGAAGAGCT TGGACAGGAC 
TTAGAGCATG TTGAGGTTTT GCAGAAGAAA TTTGAAGAGT TCCAAACAGA TCTGGCAGCT 
CATGAGGAGA GAGTGAATGA AGTGAACCAG TTCGCTGGCA AACTTATCCA GGAACAGCAC 
CCTGAGGAGG AACTTATAAA GTCCAAACAG GATGAAGTAA ATGCAAGCTG GCAGCGTCTT 
AAGGGGCTTG CCCAACAGAG GCAAGGAAAA CTCTTTGGGG CAGCTGAAGT TCAGCGCTTC 
AACAGGGATG TGGATGAAAC TATCAGCTGG ATTAAGGAGA AAGGGCAGTT GATGGCCTCA 
GATGACTTTG GCAGAGACTT GGCCAGCGTG CAAGCTTTAC TACGTAAGCA TGAAGGCCTG 
GAAAGAGATC TTGCAGCTTT GGAGGATAAG GTTAAGGCCC TCTGTGCAGA AGCTGACCGT 
TTGCAGCAGT CTCACCCTAT AAATGCTTCT CAAATTCAAG TGAAACGGGA GGAACTGATT 
GCCAACTGGG AGCAGATCCG AACTCTGGCA GCAGAGAGGC ATGCTCGCCT TAATGACTCC 
TACAGGTTGC AGCGCTTTCT TGCAGATTTC CGGGACCTCA CTAGCTGGGT AACTGAGATG 
AAGGCTCTGA TAAATGCTGA TGAGCTTGCC AATGATGTGG CTGGAGCAGA AGCCCTTCTA 
GATAGACATC AGGAACATAA GGGAGAAATT GATGCTCATG AGGATAGCTT CAGATCTGCT 
GATGAGTCTG GGCAGGCTTT GCTTGCAGCT GGGCACTATG CTTCTGATGA AGTTAAAGAA 
AAGCTGACTA TCCTCTCAGA TGAAAGATCA GCCTTGCTGG AACTATGGGA GCTTCGCAGG 
CAACAGTATG AGCAGTGCAT GGATCTGCAG CTTTTCTACA GAGATACTGA ACAAGTTGAC 
AACTGGATGA GCAAACAAGA AGCTTTTCTG CTGAACGAAG ACCTTGGTGA TTCTCTGGAT 
AGCGTGGAGG CTCTTCTAAA GAAGCATGAA GATTTTGAGA AATCCCTAAG TGCTCAAGAG 
GAGAAGATCA CAGCACTGGA TGAGTTTGCT ACTAAACTGA TTCAAAATAA CCATTATGCC 
ATGGATGACG TTGCTACGCG CAGAGATGCT CTTCTGAGTC GCCGAAATGC TCTTCATGAA 
AGAGCCATGA AGCGCCGTGC CCAACTGGCA GACTCTTTCC ATCTCCAGCA GTTTTTCAGG 
GACTCTGATG AACTGAAGAG TTGGGTTAAT GAAAAAATGA AAACTGCTAC CGATGAGGCT 
TACAAGGATC CATCCAACTT GCAAGGTAAA GTTCAGAAGC ATCAGGCTTT TGAAGCAGAG 
CTTTCTGCTA ATCAGAGTCG TATTGATGCA CTGGAGAAAG CTGGCCAGAA GTTGATTGAT 
GTCAATCACT ATGCATCTGA TGAGGTGGCA GCTCGCATGA ATGAAGTCAT CAGCTTGTGG 
AAGAAACTTC TGGAAGCCAC CGAGCTCAAA GGTATAAAGC TGCGTGAAGC CAATCAACAG 
CAGCAATTTA ATCGCAATGT AGAAGACATT GAGTTGTGGC TGTATGAAGT GGAGGGTCAC 
TTGGCTTCTG ATGATTATGG AAAAGATCTT ACCAGCGTTC AGAATCTTCA GAAGAAACAT 
GCCCTGCTAG AGGCAGATGT TGCTGCCCAT CAGGATCGGA TAGATGGCAT TACCATCCAG 
GCACGCCAGT TCCAAGATGC TGGGCACTTT GATGCTGACA ATATCAAGAA GAAACAAGAA 
GCTTTAGTAG CTCGTTATGA AGCTCTGAAG GATCCTATGG TGGCTCGCAA GCAGAAACTT 
GCAGATTCTC TTCGCCTGCA GCAGCTTTTC CGTGACATCG AGGATGAAGA GACCTGGATC 
AGGGAAAAGG AACCTATTGC AGCCTCAACA AACCGAGGCA AGGACTTAAT TGGTGTCCAG 
AATCTGCTAA AGAAGCACCA GGCTTTGCAG GCAGAAATTG CAGGCCATGA GCCTCGCATT 
AAAGCAGTCA CACAAAAAGG AAATGCGATG GTGGAAGAGG GACATTTTGC TGCTGAAGAT 
GTGAAGATCA AATTGAATGA ACTAAACCAA AAATGGGACT CTCTGAAAGC CAAGGCATCT 
CAACGGCGAC AGGATCTAGA GGATTCTCTG CAAGCTCAGC AGTATTTTGC TGATGCTAAT 
GAGGCAGAAT CATGGATGAG GGAAAAGGAG CCCATTGTAG GCAGCACGGA TTATGGAAAG 
GATGAAGACT CTGCTGAGGC TCTCCTGAAG AAGCATGAAG CTTTGATGTC TGATCTTTCT 
GCTTACGGCA GTAGCATACA GGCATTAAGG GAACAGGCCC AGTCCTGCAG GCAACAAGTT 
GCTCCCACTG ATGATGAAAC TGGAAAAGAG CTTGTGCTAG CACTCTATGA TTACCAAGAG 
AAGAGTCCTC GGGAGGTGAC TATGAAGAAA GGAGATATTC TAACCCTGCT CAACAGCACC 
AACAAGGACT GGTGGAAGGT TGAAGTTAAC GATCGTCAGG GCTTTGTACC AGCTGCCTAT 
GTGAAAAAAC TAGATCCTGC CCAGTCTGCA TCCCGAGAGA ATCTTCTAGA AGAGCAAGGC 
AGCATAGCAT TGCGACAGGA GCAAATTGAC AACCAGACTC TCATTACTAA GGAAGTCGGC 
AGTGTATCTC TGCGTATGAA ACAGGTCGAA GAACTGTATC ATTCCCTTCT TGAGCTGGGA 
GAAAAACGTA AAGGCATGCT AGAAAAAAGC TGCAAGAAGT TTATGCTTTT CCGTGAGGCT 
AATGAGCTTC AACAGTGGAT CAATGAGAAG GAAGCTGCGC TCACTAATGA GGAAGTGGGT 
GCTGATTTGG AGCAGGTGGA GGTGCTACAG AAGAAGTTTG ATGATTTTCA GAAGGATTTA 
AAAGCTAATG AGTCACGACT GAAGGACATA AACAAGGTTG CAAATGACCT GGAGTCTGAA 
GGGCTGATGG CAGAGGAAGT GCAAGCAGTA GAGCACCAGG AAGTCTATGG AATGATGCCC 
AGAGATGAAA CTGATTCTAA GACAGCCTCT CCTTGGAAGT CTGCACGTAT GATGGTACAC 
ACAGTGGCAA CGTTTAACTC AATCAAGGAA CTGAATGAAC GCTGGAGATC CCTGCAGCAG 
TTGGCAGAGG AGCGAAGCCA GCTGTTGGGC AGCGCTCATG AAGTCCAGAG GTTCCACAGA 
GATGCTGATG AAACAAAAGA ATGGATAGAA GAGAAGAATC AAGCATTAAA TACCGACAAC 
TATGGACATG ACTTGGCCAG TGTGCAGGCT CTGCAGCGCA AGCATGAAGG CTTTGAGAGA 
GACTTGGCAG CTCTTGGAGA CAAGGTGAAT TCTCTTGGTG AAACTGCCCA GCGTCTGATC 
CAGTCACATC CAGAATCAGC TGAAGATCTT CAAGAAAAAT GTACTGAGTT GAACCAAGCC 
TGGAGTAGTC TGGGAAAACG TGCTGACCAA CGCAAAGAGA AGCTTGGAGA TTCTCATGAC 
CTGCAGCGTT TCCTCAGTGA TTTTAGAGAC CTTATGTCTT GGATCAATGG AATCCGGGGT 
CTGGTCTCCT CAGATGAACT TGCAAAAGAT GTGACTGGAG CTGAAGCTCT GTTGGAAAGG 
CATCAGGAGC ACCGTACTGA AATAGATGCA CGGGCTGGCA CTTTTCAGGC ATTTGAACAG 
TTTGGACAGC AGCTTCTGGC TCGTGGACAC TATGCCAGCC CAGAGATTAA GGAGAAACTG 
GATATTCTAG ATCAAGAACG GACAGACCTA GAGAAGGCCT GGGTCCAGCG CAGAATGATG 
CTAGACCAGT GCTTAGAACT ACAGCTGTTT CATCGGGATT GTGAACAAGC TGAAAACTGG 
ATGGCTGCCC GAGAGGCGTT CCTAAATACA GAAGACAAAG GAGACTCCTT AGACAGCGTG 
GAGGCACTCA TCAAGAAGCA TGAAGATTTC GATAAAGCAA TCAATGTCCA GGAAGAGAAA 
ATTGCTGTCT TGCAGTCTTT TGCTGACCAA CTGATTGCTG CTGATCATTA TGCAAAAGGA 
GTCATTGCTA ACAGACGCAA TGAGGTTCTG GACAGGTGGC GTCGTCTGAA GGCTCAGATG 
ATTGAGAAGA GATCTAAGCT GGGAGAATCT CAGACCCTCC AACAGTTCAG TCGTGATGTT 
GATGAAATAG AAGCTTGGAT CAGTGAAAAG CTTCAAACTG CAAGTGATGA GTCATATAAG 
GATCCCACAA ACATCCAGCT TTCCAAACTG CTGAGCAAGC ACCAGAAGCA CCAAGCCTTT 
GAAGCTGAGC TCCACGCCAA CGCAGATCGG ATTCGTGGAG TCATTGAAAT GGGGAACTCT 
CTTATTGAAA GAGGAGCGTG TGCTGGCAGC GAGGATGCTG TGAAGGCTCG GCTGGCTGCC 
CTGGCTGACC AATGGGAGTT CCTGGTCCAG AAGTCATCAG AGAAGAGTCA GAAACTGAAA 
GAAGCAAATA AACAGCAGAA TTTCAATACC GGCATCAAGG ACTTTGATTT CTGGCTTTCA 
GAGGTGGAAG CTTTGTTGGC ATCTGAAGAC TATGGGAAGG ACTTGGCATC AGTGAACAAC 
CTTCTGAAGA AGCACCAATT ACTGGAAGCT GATATATCTG CTCATGAGGA TCGGCTGAAG 
GACCTGAACA GCCAGGCTGA CAGTTTGATG ACCAGCAGTG CTTTCGATAC CTCCCAAGTA 
AAGGATAAAC GTGAAACTAT AAATGGGCGC TTCCAGAGAA TCAAGAGCAT GGCAGCTGCC 
CGCCGTGCGA AGCTCAACGA GTCGCACCGC TTGCATCAGT TCTTCCGTGA CATGGATGAT 
GAGGAGTCCT GGATCAAAGA GAAGAAACTG TTGGTGAGCT CAGAGGACTA TGGCAGAGAC 
CTGACTGGTG TGCAGAACCT GAGGAAGAAA CATAAGCGCT TGGAAGCAGA ATTAGCTGCC 
CATGAACCTG CTATCCAGGG TGTTCTAGAC ACTGGCAAGA AGCTTTCAGA TGATAACACA 
ATTGGGAAGG AGGAGATACA GCAAAGACTG GCTCAGTTTG TGGACCACTG GAAAGAGTTA 
AAACAGCTGG CAGCTGCTCG GGGGCAGCGT CTGGAGGAGT CCCTGGAGTA CCAACAGTTT 
GTAGCAAATG TTGAGGAAGA GGAGGCATGG ATCAACGAGA AAATGACATT GGTAGCCAGT 
GAGGATTATG GAGACACACT TGCTGCTATC CAGGGCTTGC TGAAGAAGCA TGAAGCATTC 
GAGACTGACT TTACTGTCCA CAAAGACAGA GTGAATGATG TTTGCGCTAA TGGAGAGGAT 
CTCATTAAAA AGAATAATCA CCATGTGGAG AACATTACTG CTAAGATGAA GGGCCTCAAG 
GGCAAGGTAT CAGATCTGGA GAAAGCCGCA GCTCAGAGGA AAGCCAAACT GGATGAGAAC 
TCTGCCTTCC TCCAGTTCAA CTGGAAAGCA GATGTGGTGG AGTCATGGAT AGGTGAGAAG 
GAAAACAGTC TGAAGACAGA TGATTATGGC CGTGACCTCT CCTCTGTGCA AACACTACTC 
ACCAAACAGG AAACCTTTGA TGCTGGACTT CAGGCTTTCC AGCAGGAGGG AATTGCAAAC 
ATCACTGCTC TGAAAGACCA GCTACTGGCA GCCAAACATA TCCAATCAAA GGCCATTGAG 
GTTCGTCACG CTTCCTTGAT GAAACGCTGG AATCAGCTGC TTGCTAATTC TGCAGCCAGG 
AAAAAGAAAC TCTTGGAGGC TCAGGAGCAC TTCAGAAAGG TTGAAGATCT CTTCTTGACT 
TTTGCCAAGA AGGCCTCTGC CTTCAACAGT TGGTTTGAGA ATGCTGAGGA GGACCTGACG 
GATCCCGTGC GCTGCAATTC CCTGGAAGAA ATCAAAGCAC TGCGAGAAGC CCACGATGCG 
TTCCGTTCCT CACTTAGCTC TGCCCAAGCT GACTTCAACC AGCTGGCAGA GCTTGATCGG 
CAGATCAAGA GCTTCCGTGT AGCCTCCAAC CCGTACACTT GGTTTACTAT GGAGGCTCTT 
GAAGAAACTT GGAGGAATCT GCAGAAAATT ATCAAGGAGC GTGAATTGGA GTTGCAGAAG 
GAGCAGCGGA GGCAGGAAGA AAATGACAAG TTGCGCCAGG AGTTTGCTCA GCATGCTAAT 
GCCTTCCACC AGTGGATTCA GGAGACAAGG ACTTACCTGC TAGATGGCAT AGCCTATCGT 
CGTGTCATTC GTGTCTATCA GTATGAAGTT GGGGATGATC TATCTGGAAG GTCCTGCATG 
GTGGAGGAGT CGGGAACACT GGAATCCCAG CTGGAAGCTA CTAAACGCAA GCACCAGGAG 
ATCCGGGCTA TGAGGAGCCA GCTGAAGAAG ATTGAGGACC TTGGAGCAGC CATGGAAGAG 
GCACTTATCT TGGACAACAA ATACACAGAG CACAGCACCG TGGGGCTGGC GCAGCAGTGG 
GACCAGCTTG ACCAGCTGGG AATGAGAATG CAGCACAATC TGGAACAGCA GATTCAAGCT 
CGAAACACAA CTGGAGTCAC AGAGGAGGCC CTGAAGGAGT TCAGCATGAT GTTCAAGCAC 
TTTGACAAGG ACAAATCTGG ACGTCTTAAT CACCAGGAGT TTAAGTCTTG CTTGCGCTCC 
CTTGGCTATG ACCTGCCCAT GGTTGAGGAA GGAGAGCCTG ACCCCGAATT TGAGTCTATT 
CTTGACACTG TTGATCCCAA CAGGGATGGC CACGTCTCGC TGCAGGAGTA CATGGCGTTC 
ATGATCAGCA GGGAAACAGA GAACGTGAAA TCCAGCGAGG AGATTGAGAG TGCTTTCCGT 
GCCCTCAGCT CGGAGGGGAA GCCTTACGTG ACCAAGGAGG AGCTCTACCA GAACCTGACC 
CGGGAGCAGG CCGACTACTG CATTTCTCAC ATGAAGCCCT ACATGGATGG CAAGGGCAGA 
GAACTTCCTT CTGCCTACGA CTACATAGAA TTTACACGTT CACTCTTTGT GAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_015279290.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015134776.1 Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X9, mRNA.

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CloneID	OGa43316
Clone ID Related Accession (Same CDS sequence)	XM_015279290.2 , XM_015279290.1
Accession Version	XM_015279290.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7473bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015279290.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG 
TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC 
TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT 
GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT 
GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG 
ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAA CTCGTCTACA GGAGCTGCAC 
CGACTATGGG AGTTACTGCT GGAAAAAATG AGAGAGAAGG GAGTGAAGCT GTTGCAAGCA 
CAGAAGCTGG TGCAATTCTT ACGGGAATGT GAAGATGTCA TGGACTGGAT CAATGACAAG 
GAAGCAATAG TGACCTCAGA AGAGCTTGGA CAGGACTTAG AGCATGTTGA GGTTTTGCAG 
AAGAAATTTG AAGAGTTCCA AACAGATCTG GCAGCTCATG AGGAGAGAGT GAATGAAGTG 
AACCAGTTCG CTGGCAAACT TATCCAGGAA CAGCACCCTG AGGAGGAACT TATAAAGTCC 
AAACAGGATG AAGTAAATGC AAGCTGGCAG CGTCTTAAGG GGCTTGCCCA ACAGAGGCAA 
GGAAAACTCT TTGGGGCAGC TGAAGTTCAG CGCTTCAACA GGGATGTGGA TGAAACTATC 
AGCTGGATTA AGGAGAAAGG GCAGTTGATG GCCTCAGATG ACTTTGGCAG AGACTTGGCC 
AGCGTGCAAG CTTTACTACG TAAGCATGAA GGCCTGGAAA GAGATCTTGC AGCTTTGGAG 
GATAAGGTTA AGGCCCTCTG TGCAGAAGCT GACCGTTTGC AGCAGTCTCA CCCTATAAAT 
GCTTCTCAAA TTCAAGTGAA ACGGGAGGAA CTGATTGCCA ACTGGGAGCA GATCCGAACT 
CTGGCAGCAG AGAGGCATGC TCGCCTTAAT GACTCCTACA GGTTGCAGCG CTTTCTTGCA 
GATTTCCGGG ACCTCACTAG CTGGGTAACT GAGATGAAGG CTCTGATAAA TGCTGATGAG 
CTTGCCAATG ATGTGGCTGG AGCAGAAGCC CTTCTAGATA GACATCAGGA ACATAAGGGA 
GAAATTGATG CTCATGAGGA TAGCTTCAGA TCTGCTGATG AGTCTGGGCA GGCTTTGCTT 
GCAGCTGGGC ACTATGCTTC TGATGAAGTT AAAGAAAAGC TGACTATCCT CTCAGATGAA 
AGATCAGCCT TGCTGGAACT ATGGGAGCTT CGCAGGCAAC AGTATGAGCA GTGCATGGAT 
CTGCAGCTTT TCTACAGAGA TACTGAACAA GTTGACAACT GGATGAGCAA ACAAGAAGCT 
TTTCTGCTGA ACGAAGACCT TGGTGATTCT CTGGATAGCG TGGAGGCTCT TCTAAAGAAG 
CATGAAGATT TTGAGAAATC CCTAAGTGCT CAAGAGGAGA AGATCACAGC ACTGGATGAG 
TTTGCTACTA AACTGATTCA AAATAACCAT TATGCCATGG ATGACGTTGC TACGCGCAGA 
GATGCTCTTC TGAGTCGCCG AAATGCTCTT CATGAAAGAG CCATGAAGCG CCGTGCCCAA 
CTGGCAGACT CTTTCCATCT CCAGCAGTTT TTCAGGGACT CTGATGAACT GAAGAGTTGG 
GTTAATGAAA AAATGAAAAC TGCTACCGAT GAGGCTTACA AGGATCCATC CAACTTGCAA 
GGTAAAGTTC AGAAGCATCA GGCTTTTGAA GCAGAGCTTT CTGCTAATCA GAGTCGTATT 
GATGCACTGG AGAAAGCTGG CCAGAAGTTG ATTGATGTCA ATCACTATGC ATCTGATGAG 
GTGGCAGCTC GCATGAATGA AGTCATCAGC TTGTGGAAGA AACTTCTGGA AGCCACCGAG 
CTCAAAGGTA TAAAGCTGCG TGAAGCCAAT CAACAGCAGC AATTTAATCG CAATGTAGAA 
GACATTGAGT TGTGGCTGTA TGAAGTGGAG GGTCACTTGG CTTCTGATGA TTATGGAAAA 
GATCTTACCA GCGTTCAGAA TCTTCAGAAG AAACATGCCC TGCTAGAGGC AGATGTTGCT 
GCCCATCAGG ATCGGATAGA TGGCATTACC ATCCAGGCAC GCCAGTTCCA AGATGCTGGG 
CACTTTGATG CTGACAATAT CAAGAAGAAA CAAGAAGCTT TAGTAGCTCG TTATGAAGCT 
CTGAAGGATC CTATGGTGGC TCGCAAGCAG AAACTTGCAG ATTCTCTTCG CCTGCAGCAG 
CTTTTCCGTG ACATCGAGGA TGAAGAGACC TGGATCAGGG AAAAGGAACC TATTGCAGCC 
TCAACAAACC GAGGCAAGGA CTTAATTGGT GTCCAGAATC TGCTAAAGAA GCACCAGGCT 
TTGCAGGCAG AAATTGCAGG CCATGAGCCT CGCATTAAAG CAGTCACACA AAAAGGAAAT 
GCGATGGTGG AAGAGGGACA TTTTGCTGCT GAAGATGTGA AGATCAAATT GAATGAACTA 
AACCAAAAAT GGGACTCTCT GAAAGCCAAG GCATCTCAAC GGCGACAGGA TCTAGAGGAT 
TCTCTGCAAG CTCAGCAGTA TTTTGCTGAT GCTAATGAGG CAGAATCATG GATGAGGGAA 
AAGGAGCCCA TTGTAGGCAG CACGGATTAT GGAAAGGATG AAGACTCTGC TGAGGCTCTC 
CTGAAGAAGC ATGAAGCTTT GATGTCTGAT CTTTCTGCTT ACGGCAGTAG CATACAGGCA 
TTAAGGGAAC AGGCCCAGTC CTGCAGGCAA CAAGTTGCTC CCACTGATGA TGAAACTGGA 
AAAGAGCTTG TGCTAGCACT CTATGATTAC CAAGAGAAGA GTCCTCGGGA GGTGACTATG 
AAGAAAGGAG ATATTCTAAC CCTGCTCAAC AGCACCAACA AGGACTGGTG GAAGGTTGAA 
GTTAACGATC GTCAGGGCTT TGTACCAGCT GCCTATGTGA AAAAACTAGA TCCTGCCCAG 
TCTGCATCCC GAGAGAATCT TCTAGAAGAG CAAGGCAGCA TAGCATTGCG ACAGGAGCAA 
ATTGACAACC AGTATCATTC CCTTCTTGAG CTGGGAGAAA AACGTAAAGG CATGCTAGAA 
AAAAGCTGCA AGAAGTTTAT GCTTTTCCGT GAGGCTAATG AGCTTCAACA GTGGATCAAT 
GAGAAGGAAG CTGCGCTCAC TAATGAGGAA GTGGGTGCTG ATTTGGAGCA GGTGGAGGTG 
CTACAGAAGA AGTTTGATGA TTTTCAGAAG GATTTAAAAG CTAATGAGTC ACGACTGAAG 
GACATAAACA AGGTTGCAAA TGACCTGGAG TCTGAAGGGC TGATGGCAGA GGAAGTGCAA 
GCAGTAGAGC ACCAGGAAGT CTATGGAATG ATGCCCAGAG ATGAAACTGA TTCTAAGACA 
GCCTCTCCTT GGAAGTCTGC ACGTATGATG GTACACACAG TGGCAACGTT TAACTCAATC 
AAGGAACTGA ATGAACGCTG GAGATCCCTG CAGCAGTTGG CAGAGGAGCG AAGCCAGCTG 
TTGGGCAGCG CTCATGAAGT CCAGAGGTTC CACAGAGATG CTGATGAAAC AAAAGAATGG 
ATAGAAGAGA AGAATCAAGC ATTAAATACC GACAACTATG GACATGACTT GGCCAGTGTG 
CAGGCTCTGC AGCGCAAGCA TGAAGGCTTT GAGAGAGACT TGGCAGCTCT TGGAGACAAG 
GTGAATTCTC TTGGTGAAAC TGCCCAGCGT CTGATCCAGT CACATCCAGA ATCAGCTGAA 
GATCTTCAAG AAAAATGTAC TGAGTTGAAC CAAGCCTGGA GTAGTCTGGG AAAACGTGCT 
GACCAACGCA AAGAGAAGCT TGGAGATTCT CATGACCTGC AGCGTTTCCT CAGTGATTTT 
AGAGACCTTA TGTCTTGGAT CAATGGAATC CGGGGTCTGG TCTCCTCAGA TGAACTTGCA 
AAAGATGTGA CTGGAGCTGA AGCTCTGTTG GAAAGGCATC AGGAGCACCG TACTGAAATA 
GATGCACGGG CTGGCACTTT TCAGGCATTT GAACAGTTTG GACAGCAGCT TCTGGCTCGT 
GGACACTATG CCAGCCCAGA GATTAAGGAG AAACTGGATA TTCTAGATCA AGAACGGACA 
GACCTAGAGA AGGCCTGGGT CCAGCGCAGA ATGATGCTAG ACCAGTGCTT AGAACTACAG 
CTGTTTCATC GGGATTGTGA ACAAGCTGAA AACTGGATGG CTGCCCGAGA GGCGTTCCTA 
AATACAGAAG ACAAAGGAGA CTCCTTAGAC AGCGTGGAGG CACTCATCAA GAAGCATGAA 
GATTTCGATA AAGCAATCAA TGTCCAGGAA GAGAAAATTG CTGTCTTGCA GTCTTTTGCT 
GACCAACTGA TTGCTGCTGA TCATTATGCA AAAGGAGTCA TTGCTAACAG ACGCAATGAG 
GTTCTGGACA GGTGGCGTCG TCTGAAGGCT CAGATGATTG AGAAGAGATC TAAGCTGGGA 
GAATCTCAGA CCCTCCAACA GTTCAGTCGT GATGTTGATG AAATAGAAGC TTGGATCAGT 
GAAAAGCTTC AAACTGCAAG TGATGAGTCA TATAAGGATC CCACAAACAT CCAGCTTTCC 
AAACTGCTGA GCAAGCACCA GAAGCACCAA GCCTTTGAAG CTGAGCTCCA CGCCAACGCA 
GATCGGATTC GTGGAGTCAT TGAAATGGGG AACTCTCTTA TTGAAAGAGG AGCGTGTGCT 
GGCAGCGAGG ATGCTGTGAA GGCTCGGCTG GCTGCCCTGG CTGACCAATG GGAGTTCCTG 
GTCCAGAAGT CATCAGAGAA GAGTCAGAAA CTGAAAGAAG CAAATAAACA GCAGAATTTC 
AATACCGGCA TCAAGGACTT TGATTTCTGG CTTTCAGAGG TGGAAGCTTT GTTGGCATCT 
GAAGACTATG GGAAGGACTT GGCATCAGTG AACAACCTTC TGAAGAAGCA CCAATTACTG 
GAAGCTGATA TATCTGCTCA TGAGGATCGG CTGAAGGACC TGAACAGCCA GGCTGACAGT 
TTGATGACCA GCAGTGCTTT CGATACCTCC CAAGTAAAGG ATAAACGTGA AACTATAAAT 
GGGCGCTTCC AGAGAATCAA GAGCATGGCA GCTGCCCGCC GTGCGAAGCT CAACGAGTCG 
CACCGCTTGC ATCAGTTCTT CCGTGACATG GATGATGAGG AGTCCTGGAT CAAAGAGAAG 
AAACTGTTGG TGAGCTCAGA GGACTATGGC AGAGACCTGA CTGGTGTGCA GAACCTGAGG 
AAGAAACATA AGCGCTTGGA AGCAGAATTA GCTGCCCATG AACCTGCTAT CCAGGGTGTT 
CTAGACACTG GCAAGAAGCT TTCAGATGAT AACACAATTG GGAAGGAGGA GATACAGCAA 
AGACTGGCTC AGTTTGTGGA CCACTGGAAA GAGTTAAAAC AGCTGGCAGC TGCTCGGGGG 
CAGCGTCTGG AGGAGTCCCT GGAGTACCAA CAGTTTGTAG CAAATGTTGA GGAAGAGGAG 
GCATGGATCA ACGAGAAAAT GACATTGGTA GCCAGTGAGG ATTATGGAGA CACACTTGCT 
GCTATCCAGG GCTTGCTGAA GAAGCATGAA GCATTCGAGA CTGACTTTAC TGTCCACAAA 
GACAGAGTGA ATGATGTTTG CGCTAATGGA GAGGATCTCA TTAAAAAGAA TAATCACCAT 
GTGGAGAACA TTACTGCTAA GATGAAGGGC CTCAAGGGCA AGGTATCAGA TCTGGAGAAA 
GCCGCAGCTC AGAGGAAAGC CAAACTGGAT GAGAACTCTG CCTTCCTCCA GTTCAACTGG 
AAAGCAGATG TGGTGGAGTC ATGGATAGGT GAGAAGGAAA ACAGTCTGAA GACAGATGAT 
TATGGCCGTG ACCTCTCCTC TGTGCAAACA CTACTCACCA AACAGGAAAC CTTTGATGCT 
GGACTTCAGG CTTTCCAGCA GGAGGGAATT GCAAACATCA CTGCTCTGAA AGACCAGCTA 
CTGGCAGCCA AACATATCCA ATCAAAGGCC ATTGAGGTTC GTCACGCTTC CTTGATGAAA 
CGCTGGAATC AGCTGCTTGC TAATTCTGCA GCCAGGAAAA AGAAACTCTT GGAGGCTCAG 
GAGCACTTCA GAAAGGTTGA AGATCTCTTC TTGACTTTTG CCAAGAAGGC CTCTGCCTTC 
AACAGTTGGT TTGAGAATGC TGAGGAGGAC CTGACGGATC CCGTGCGCTG CAATTCCCTG 
GAAGAAATCA AAGCACTGCG AGAAGCCCAC GATGCGTTCC GTTCCTCACT TAGCTCTGCC 
CAAGCTGACT TCAACCAGCT GGCAGAGCTT GATCGGCAGA TCAAGAGCTT CCGTGTAGCC 
TCCAACCCGT ACACTTGGTT TACTATGGAG GCTCTTGAAG AAACTTGGAG GAATCTGCAG 
AAAATTATCA AGGAGCGTGA ATTGGAGTTG CAGAAGGAGC AGCGGAGGCA GGAAGAAAAT 
GACAAGTTGC GCCAGGAGTT TGCTCAGCAT GCTAATGCCT TCCACCAGTG GATTCAGGAG 
ACAAGGACTT ACCTGCTAGA TGGCATAGCC TATCGTCGTG TCATTCGTGT CTATCAGTAT 
GAAGTTGGGG ATGATCTATC TGGAAGGTCC TGCATGGTGG AGGAGTCGGG AACACTGGAA 
TCCCAGCTGG AAGCTACTAA ACGCAAGCAC CAGGAGATCC GGGCTATGAG GAGCCAGCTG 
AAGAAGATTG AGGACCTTGG AGCAGCCATG GAAGAGGCAC TTATCTTGGA CAACAAATAC 
ACAGAGCACA GCACCGTGGG GCTGGCGCAG CAGTGGGACC AGCTTGACCA GCTGGGAATG 
AGAATGCAGC ACAATCTGGA ACAGCAGATT CAAGCTCGAA ACACAACTGG AGTCACAGAG 
GAGGCCCTGA AGGAGTTCAG CATGATGTTC AAGCACTTTG ACAAGGACAA ATCTGGACGT 
CTTAATCACC AGGAGTTTAA GTCTTGCTTG CGCTCCCTTG GCTATGACCT GCCCATGGTT 
GAGGAAGGAG AGCCTGACCC CGAATTTGAG TCTATTCTTG ACACTGTTGA TCCCAACAGG 
GATGGCCACG TCTCGCTGCA GGAGTACATG GCGTTCATGA TCAGCAGGGA AACAGAGAAC 
GTGAAATCCA GCGAGGAGAT TGAGAGTGCT TTCCGTGCCC TCAGCTCGGA GGGGAAGCCT 
TACGTGACCA AGGAGGAGCT CTACCAGAAC CTGACCCGGG AGCAGGCCGA CTACTGCATT 
TCTCACATGA AGCCCTACAT GGATGGCAAG GGCAGAGAAC TTCCTTCTGC CTACGACTAC 
ATAGAATTTA CACGTTCACT CTTTGTGAAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015134776.1
CDS	13..7485
Translation	MLSSFRKVKVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSSLRRQKLEDSYRFQFFQRDADELEKWIQEKLQIASDENYKDPSNLQGKLQKHQAFEAEVQANSGAIVKLDETGNQMINEGHFASETIRTRLQELHRLWELLLEKMREKGVKLLQAQKLVQFLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDLAAHEERVNEVNQFAGKLIQEQHPEEELIKSKQDEVNASWQRLKGLAQQRQGKLFGAAEVQRFNRDVDETISWIKEKGQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPINASQIQVKREELIANWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFRSADESGQALLAAGHYASDEVKEKLTILSDERSALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMDDVATRRDALLSRRNALHERAMKRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYASDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTSVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDADNIKKKQEALVARYEALKDPMVARKQKLADSLRLQQLFRDIEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKIKLNELNQKWDSLKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPTDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTNEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVANDLESEGLMAEEVQAVEHQEVYGMMPRDETDSKTASPWKSARMMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAQRLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKEKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLARGHYASPEIKEKLDILDQERTDLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAVLQSFADQLIAADHYAKGVIANRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIEMGNSLIERGACAGSEDAVKARLAALADQWEFLVQKSSEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRETINGRFQRIKSMAAARRAKLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVDHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCANGEDLIKKNNHHVENITAKMKGLKGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHIQSKAIEVRHASLMKRWNQLLANSAARKKKLLEAQEHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGIAYRRVIRVYQYEVGDDLSGRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFESILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCISHMKPYMDGKGRELPSAYDYIEFTRSLFVN

Target ORF information:

RefSeq Version	XM_015279290.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279290.2

ORF Insert Sequence:

ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG 
TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC 
TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT 
GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT 
GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG 
ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAA CTCGTCTACA GGAGCTGCAC 
CGACTATGGG AGTTACTGCT GGAAAAAATG AGAGAGAAGG GAGTGAAGCT GTTGCAAGCA 
CAGAAGCTGG TGCAATTCTT ACGGGAATGT GAAGATGTCA TGGACTGGAT CAATGACAAG 
GAAGCAATAG TGACCTCAGA AGAGCTTGGA CAGGACTTAG AGCATGTTGA GGTTTTGCAG 
AAGAAATTTG AAGAGTTCCA AACAGATCTG GCAGCTCATG AGGAGAGAGT GAATGAAGTG 
AACCAGTTCG CTGGCAAACT TATCCAGGAA CAGCACCCTG AGGAGGAACT TATAAAGTCC 
AAACAGGATG AAGTAAATGC AAGCTGGCAG CGTCTTAAGG GGCTTGCCCA ACAGAGGCAA 
GGAAAACTCT TTGGGGCAGC TGAAGTTCAG CGCTTCAACA GGGATGTGGA TGAAACTATC 
AGCTGGATTA AGGAGAAAGG GCAGTTGATG GCCTCAGATG ACTTTGGCAG AGACTTGGCC 
AGCGTGCAAG CTTTACTACG TAAGCATGAA GGCCTGGAAA GAGATCTTGC AGCTTTGGAG 
GATAAGGTTA AGGCCCTCTG TGCAGAAGCT GACCGTTTGC AGCAGTCTCA CCCTATAAAT 
GCTTCTCAAA TTCAAGTGAA ACGGGAGGAA CTGATTGCCA ACTGGGAGCA GATCCGAACT 
CTGGCAGCAG AGAGGCATGC TCGCCTTAAT GACTCCTACA GGTTGCAGCG CTTTCTTGCA 
GATTTCCGGG ACCTCACTAG CTGGGTAACT GAGATGAAGG CTCTGATAAA TGCTGATGAG 
CTTGCCAATG ATGTGGCTGG AGCAGAAGCC CTTCTAGATA GACATCAGGA ACATAAGGGA 
GAAATTGATG CTCATGAGGA TAGCTTCAGA TCTGCTGATG AGTCTGGGCA GGCTTTGCTT 
GCAGCTGGGC ACTATGCTTC TGATGAAGTT AAAGAAAAGC TGACTATCCT CTCAGATGAA 
AGATCAGCCT TGCTGGAACT ATGGGAGCTT CGCAGGCAAC AGTATGAGCA GTGCATGGAT 
CTGCAGCTTT TCTACAGAGA TACTGAACAA GTTGACAACT GGATGAGCAA ACAAGAAGCT 
TTTCTGCTGA ACGAAGACCT TGGTGATTCT CTGGATAGCG TGGAGGCTCT TCTAAAGAAG 
CATGAAGATT TTGAGAAATC CCTAAGTGCT CAAGAGGAGA AGATCACAGC ACTGGATGAG 
TTTGCTACTA AACTGATTCA AAATAACCAT TATGCCATGG ATGACGTTGC TACGCGCAGA 
GATGCTCTTC TGAGTCGCCG AAATGCTCTT CATGAAAGAG CCATGAAGCG CCGTGCCCAA 
CTGGCAGACT CTTTCCATCT CCAGCAGTTT TTCAGGGACT CTGATGAACT GAAGAGTTGG 
GTTAATGAAA AAATGAAAAC TGCTACCGAT GAGGCTTACA AGGATCCATC CAACTTGCAA 
GGTAAAGTTC AGAAGCATCA GGCTTTTGAA GCAGAGCTTT CTGCTAATCA GAGTCGTATT 
GATGCACTGG AGAAAGCTGG CCAGAAGTTG ATTGATGTCA ATCACTATGC ATCTGATGAG 
GTGGCAGCTC GCATGAATGA AGTCATCAGC TTGTGGAAGA AACTTCTGGA AGCCACCGAG 
CTCAAAGGTA TAAAGCTGCG TGAAGCCAAT CAACAGCAGC AATTTAATCG CAATGTAGAA 
GACATTGAGT TGTGGCTGTA TGAAGTGGAG GGTCACTTGG CTTCTGATGA TTATGGAAAA 
GATCTTACCA GCGTTCAGAA TCTTCAGAAG AAACATGCCC TGCTAGAGGC AGATGTTGCT 
GCCCATCAGG ATCGGATAGA TGGCATTACC ATCCAGGCAC GCCAGTTCCA AGATGCTGGG 
CACTTTGATG CTGACAATAT CAAGAAGAAA CAAGAAGCTT TAGTAGCTCG TTATGAAGCT 
CTGAAGGATC CTATGGTGGC TCGCAAGCAG AAACTTGCAG ATTCTCTTCG CCTGCAGCAG 
CTTTTCCGTG ACATCGAGGA TGAAGAGACC TGGATCAGGG AAAAGGAACC TATTGCAGCC 
TCAACAAACC GAGGCAAGGA CTTAATTGGT GTCCAGAATC TGCTAAAGAA GCACCAGGCT 
TTGCAGGCAG AAATTGCAGG CCATGAGCCT CGCATTAAAG CAGTCACACA AAAAGGAAAT 
GCGATGGTGG AAGAGGGACA TTTTGCTGCT GAAGATGTGA AGATCAAATT GAATGAACTA 
AACCAAAAAT GGGACTCTCT GAAAGCCAAG GCATCTCAAC GGCGACAGGA TCTAGAGGAT 
TCTCTGCAAG CTCAGCAGTA TTTTGCTGAT GCTAATGAGG CAGAATCATG GATGAGGGAA 
AAGGAGCCCA TTGTAGGCAG CACGGATTAT GGAAAGGATG AAGACTCTGC TGAGGCTCTC 
CTGAAGAAGC ATGAAGCTTT GATGTCTGAT CTTTCTGCTT ACGGCAGTAG CATACAGGCA 
TTAAGGGAAC AGGCCCAGTC CTGCAGGCAA CAAGTTGCTC CCACTGATGA TGAAACTGGA 
AAAGAGCTTG TGCTAGCACT CTATGATTAC CAAGAGAAGA GTCCTCGGGA GGTGACTATG 
AAGAAAGGAG ATATTCTAAC CCTGCTCAAC AGCACCAACA AGGACTGGTG GAAGGTTGAA 
GTTAACGATC GTCAGGGCTT TGTACCAGCT GCCTATGTGA AAAAACTAGA TCCTGCCCAG 
TCTGCATCCC GAGAGAATCT TCTAGAAGAG CAAGGCAGCA TAGCATTGCG ACAGGAGCAA 
ATTGACAACC AGTATCATTC CCTTCTTGAG CTGGGAGAAA AACGTAAAGG CATGCTAGAA 
AAAAGCTGCA AGAAGTTTAT GCTTTTCCGT GAGGCTAATG AGCTTCAACA GTGGATCAAT 
GAGAAGGAAG CTGCGCTCAC TAATGAGGAA GTGGGTGCTG ATTTGGAGCA GGTGGAGGTG 
CTACAGAAGA AGTTTGATGA TTTTCAGAAG GATTTAAAAG CTAATGAGTC ACGACTGAAG 
GACATAAACA AGGTTGCAAA TGACCTGGAG TCTGAAGGGC TGATGGCAGA GGAAGTGCAA 
GCAGTAGAGC ACCAGGAAGT CTATGGAATG ATGCCCAGAG ATGAAACTGA TTCTAAGACA 
GCCTCTCCTT GGAAGTCTGC ACGTATGATG GTACACACAG TGGCAACGTT TAACTCAATC 
AAGGAACTGA ATGAACGCTG GAGATCCCTG CAGCAGTTGG CAGAGGAGCG AAGCCAGCTG 
TTGGGCAGCG CTCATGAAGT CCAGAGGTTC CACAGAGATG CTGATGAAAC AAAAGAATGG 
ATAGAAGAGA AGAATCAAGC ATTAAATACC GACAACTATG GACATGACTT GGCCAGTGTG 
CAGGCTCTGC AGCGCAAGCA TGAAGGCTTT GAGAGAGACT TGGCAGCTCT TGGAGACAAG 
GTGAATTCTC TTGGTGAAAC TGCCCAGCGT CTGATCCAGT CACATCCAGA ATCAGCTGAA 
GATCTTCAAG AAAAATGTAC TGAGTTGAAC CAAGCCTGGA GTAGTCTGGG AAAACGTGCT 
GACCAACGCA AAGAGAAGCT TGGAGATTCT CATGACCTGC AGCGTTTCCT CAGTGATTTT 
AGAGACCTTA TGTCTTGGAT CAATGGAATC CGGGGTCTGG TCTCCTCAGA TGAACTTGCA 
AAAGATGTGA CTGGAGCTGA AGCTCTGTTG GAAAGGCATC AGGAGCACCG TACTGAAATA 
GATGCACGGG CTGGCACTTT TCAGGCATTT GAACAGTTTG GACAGCAGCT TCTGGCTCGT 
GGACACTATG CCAGCCCAGA GATTAAGGAG AAACTGGATA TTCTAGATCA AGAACGGACA 
GACCTAGAGA AGGCCTGGGT CCAGCGCAGA ATGATGCTAG ACCAGTGCTT AGAACTACAG 
CTGTTTCATC GGGATTGTGA ACAAGCTGAA AACTGGATGG CTGCCCGAGA GGCGTTCCTA 
AATACAGAAG ACAAAGGAGA CTCCTTAGAC AGCGTGGAGG CACTCATCAA GAAGCATGAA 
GATTTCGATA AAGCAATCAA TGTCCAGGAA GAGAAAATTG CTGTCTTGCA GTCTTTTGCT 
GACCAACTGA TTGCTGCTGA TCATTATGCA AAAGGAGTCA TTGCTAACAG ACGCAATGAG 
GTTCTGGACA GGTGGCGTCG TCTGAAGGCT CAGATGATTG AGAAGAGATC TAAGCTGGGA 
GAATCTCAGA CCCTCCAACA GTTCAGTCGT GATGTTGATG AAATAGAAGC TTGGATCAGT 
GAAAAGCTTC AAACTGCAAG TGATGAGTCA TATAAGGATC CCACAAACAT CCAGCTTTCC 
AAACTGCTGA GCAAGCACCA GAAGCACCAA GCCTTTGAAG CTGAGCTCCA CGCCAACGCA 
GATCGGATTC GTGGAGTCAT TGAAATGGGG AACTCTCTTA TTGAAAGAGG AGCGTGTGCT 
GGCAGCGAGG ATGCTGTGAA GGCTCGGCTG GCTGCCCTGG CTGACCAATG GGAGTTCCTG 
GTCCAGAAGT CATCAGAGAA GAGTCAGAAA CTGAAAGAAG CAAATAAACA GCAGAATTTC 
AATACCGGCA TCAAGGACTT TGATTTCTGG CTTTCAGAGG TGGAAGCTTT GTTGGCATCT 
GAAGACTATG GGAAGGACTT GGCATCAGTG AACAACCTTC TGAAGAAGCA CCAATTACTG 
GAAGCTGATA TATCTGCTCA TGAGGATCGG CTGAAGGACC TGAACAGCCA GGCTGACAGT 
TTGATGACCA GCAGTGCTTT CGATACCTCC CAAGTAAAGG ATAAACGTGA AACTATAAAT 
GGGCGCTTCC AGAGAATCAA GAGCATGGCA GCTGCCCGCC GTGCGAAGCT CAACGAGTCG 
CACCGCTTGC ATCAGTTCTT CCGTGACATG GATGATGAGG AGTCCTGGAT CAAAGAGAAG 
AAACTGTTGG TGAGCTCAGA GGACTATGGC AGAGACCTGA CTGGTGTGCA GAACCTGAGG 
AAGAAACATA AGCGCTTGGA AGCAGAATTA GCTGCCCATG AACCTGCTAT CCAGGGTGTT 
CTAGACACTG GCAAGAAGCT TTCAGATGAT AACACAATTG GGAAGGAGGA GATACAGCAA 
AGACTGGCTC AGTTTGTGGA CCACTGGAAA GAGTTAAAAC AGCTGGCAGC TGCTCGGGGG 
CAGCGTCTGG AGGAGTCCCT GGAGTACCAA CAGTTTGTAG CAAATGTTGA GGAAGAGGAG 
GCATGGATCA ACGAGAAAAT GACATTGGTA GCCAGTGAGG ATTATGGAGA CACACTTGCT 
GCTATCCAGG GCTTGCTGAA GAAGCATGAA GCATTCGAGA CTGACTTTAC TGTCCACAAA 
GACAGAGTGA ATGATGTTTG CGCTAATGGA GAGGATCTCA TTAAAAAGAA TAATCACCAT 
GTGGAGAACA TTACTGCTAA GATGAAGGGC CTCAAGGGCA AGGTATCAGA TCTGGAGAAA 
GCCGCAGCTC AGAGGAAAGC CAAACTGGAT GAGAACTCTG CCTTCCTCCA GTTCAACTGG 
AAAGCAGATG TGGTGGAGTC ATGGATAGGT GAGAAGGAAA ACAGTCTGAA GACAGATGAT 
TATGGCCGTG ACCTCTCCTC TGTGCAAACA CTACTCACCA AACAGGAAAC CTTTGATGCT 
GGACTTCAGG CTTTCCAGCA GGAGGGAATT GCAAACATCA CTGCTCTGAA AGACCAGCTA 
CTGGCAGCCA AACATATCCA ATCAAAGGCC ATTGAGGTTC GTCACGCTTC CTTGATGAAA 
CGCTGGAATC AGCTGCTTGC TAATTCTGCA GCCAGGAAAA AGAAACTCTT GGAGGCTCAG 
GAGCACTTCA GAAAGGTTGA AGATCTCTTC TTGACTTTTG CCAAGAAGGC CTCTGCCTTC 
AACAGTTGGT TTGAGAATGC TGAGGAGGAC CTGACGGATC CCGTGCGCTG CAATTCCCTG 
GAAGAAATCA AAGCACTGCG AGAAGCCCAC GATGCGTTCC GTTCCTCACT TAGCTCTGCC 
CAAGCTGACT TCAACCAGCT GGCAGAGCTT GATCGGCAGA TCAAGAGCTT CCGTGTAGCC 
TCCAACCCGT ACACTTGGTT TACTATGGAG GCTCTTGAAG AAACTTGGAG GAATCTGCAG 
AAAATTATCA AGGAGCGTGA ATTGGAGTTG CAGAAGGAGC AGCGGAGGCA GGAAGAAAAT 
GACAAGTTGC GCCAGGAGTT TGCTCAGCAT GCTAATGCCT TCCACCAGTG GATTCAGGAG 
ACAAGGACTT ACCTGCTAGA TGGCATAGCC TATCGTCGTG TCATTCGTGT CTATCAGTAT 
GAAGTTGGGG ATGATCTATC TGGAAGGTCC TGCATGGTGG AGGAGTCGGG AACACTGGAA 
TCCCAGCTGG AAGCTACTAA ACGCAAGCAC CAGGAGATCC GGGCTATGAG GAGCCAGCTG 
AAGAAGATTG AGGACCTTGG AGCAGCCATG GAAGAGGCAC TTATCTTGGA CAACAAATAC 
ACAGAGCACA GCACCGTGGG GCTGGCGCAG CAGTGGGACC AGCTTGACCA GCTGGGAATG 
AGAATGCAGC ACAATCTGGA ACAGCAGATT CAAGCTCGAA ACACAACTGG AGTCACAGAG 
GAGGCCCTGA AGGAGTTCAG CATGATGTTC AAGCACTTTG ACAAGGACAA ATCTGGACGT 
CTTAATCACC AGGAGTTTAA GTCTTGCTTG CGCTCCCTTG GCTATGACCT GCCCATGGTT 
GAGGAAGGAG AGCCTGACCC CGAATTTGAG TCTATTCTTG ACACTGTTGA TCCCAACAGG 
GATGGCCACG TCTCGCTGCA GGAGTACATG GCGTTCATGA TCAGCAGGGA AACAGAGAAC 
GTGAAATCCA GCGAGGAGAT TGAGAGTGCT TTCCGTGCCC TCAGCTCGGA GGGGAAGCCT 
TACGTGACCA AGGAGGAGCT CTACCAGAAC CTGACCCGGG AGCAGGCCGA CTACTGCATT 
TCTCACATGA AGCCCTACAT GGATGGCAAG GGCAGAGAAC TTCCTTCTGC CTACGACTAC 
ATAGAATTTA CACGTTCACT CTTTGTGAAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_015279290.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015134776.1 Gallus gallus spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X9, mRNA.

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CloneID	OGa43316
Clone ID Related Accession (Same CDS sequence)	XM_015279290.2 , XM_015279290.1
Accession Version	XM_015279290.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7473bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG 
TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC 
TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT 
GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT 
GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG 
ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAA CTCGTCTACA GGAGCTGCAC 
CGACTATGGG AGTTACTGCT GGAAAAAATG AGAGAGAAGG GAGTGAAGCT GTTGCAAGCA 
CAGAAGCTGG TGCAATTCTT ACGGGAATGT GAAGATGTCA TGGACTGGAT CAATGACAAG 
GAAGCAATAG TGACCTCAGA AGAGCTTGGA CAGGACTTAG AGCATGTTGA GGTTTTGCAG 
AAGAAATTTG AAGAGTTCCA AACAGATCTG GCAGCTCATG AGGAGAGAGT GAATGAAGTG 
AACCAGTTCG CTGGCAAACT TATCCAGGAA CAGCACCCTG AGGAGGAACT TATAAAGTCC 
AAACAGGATG AAGTAAATGC AAGCTGGCAG CGTCTTAAGG GGCTTGCCCA ACAGAGGCAA 
GGAAAACTCT TTGGGGCAGC TGAAGTTCAG CGCTTCAACA GGGATGTGGA TGAAACTATC 
AGCTGGATTA AGGAGAAAGG GCAGTTGATG GCCTCAGATG ACTTTGGCAG AGACTTGGCC 
AGCGTGCAAG CTTTACTACG TAAGCATGAA GGCCTGGAAA GAGATCTTGC AGCTTTGGAG 
GATAAGGTTA AGGCCCTCTG TGCAGAAGCT GACCGTTTGC AGCAGTCTCA CCCTATAAAT 
GCTTCTCAAA TTCAAGTGAA ACGGGAGGAA CTGATTGCCA ACTGGGAGCA GATCCGAACT 
CTGGCAGCAG AGAGGCATGC TCGCCTTAAT GACTCCTACA GGTTGCAGCG CTTTCTTGCA 
GATTTCCGGG ACCTCACTAG CTGGGTAACT GAGATGAAGG CTCTGATAAA TGCTGATGAG 
CTTGCCAATG ATGTGGCTGG AGCAGAAGCC CTTCTAGATA GACATCAGGA ACATAAGGGA 
GAAATTGATG CTCATGAGGA TAGCTTCAGA TCTGCTGATG AGTCTGGGCA GGCTTTGCTT 
GCAGCTGGGC ACTATGCTTC TGATGAAGTT AAAGAAAAGC TGACTATCCT CTCAGATGAA 
AGATCAGCCT TGCTGGAACT ATGGGAGCTT CGCAGGCAAC AGTATGAGCA GTGCATGGAT 
CTGCAGCTTT TCTACAGAGA TACTGAACAA GTTGACAACT GGATGAGCAA ACAAGAAGCT 
TTTCTGCTGA ACGAAGACCT TGGTGATTCT CTGGATAGCG TGGAGGCTCT TCTAAAGAAG 
CATGAAGATT TTGAGAAATC CCTAAGTGCT CAAGAGGAGA AGATCACAGC ACTGGATGAG 
TTTGCTACTA AACTGATTCA AAATAACCAT TATGCCATGG ATGACGTTGC TACGCGCAGA 
GATGCTCTTC TGAGTCGCCG AAATGCTCTT CATGAAAGAG CCATGAAGCG CCGTGCCCAA 
CTGGCAGACT CTTTCCATCT CCAGCAGTTT TTCAGGGACT CTGATGAACT GAAGAGTTGG 
GTTAATGAAA AAATGAAAAC TGCTACCGAT GAGGCTTACA AGGATCCATC CAACTTGCAA 
GGTAAAGTTC AGAAGCATCA GGCTTTTGAA GCAGAGCTTT CTGCTAATCA GAGTCGTATT 
GATGCACTGG AGAAAGCTGG CCAGAAGTTG ATTGATGTCA ATCACTATGC ATCTGATGAG 
GTGGCAGCTC GCATGAATGA AGTCATCAGC TTGTGGAAGA AACTTCTGGA AGCCACCGAG 
CTCAAAGGTA TAAAGCTGCG TGAAGCCAAT CAACAGCAGC AATTTAATCG CAATGTAGAA 
GACATTGAGT TGTGGCTGTA TGAAGTGGAG GGTCACTTGG CTTCTGATGA TTATGGAAAA 
GATCTTACCA GCGTTCAGAA TCTTCAGAAG AAACATGCCC TGCTAGAGGC AGATGTTGCT 
GCCCATCAGG ATCGGATAGA TGGCATTACC ATCCAGGCAC GCCAGTTCCA AGATGCTGGG 
CACTTTGATG CTGACAATAT CAAGAAGAAA CAAGAAGCTT TAGTAGCTCG TTATGAAGCT 
CTGAAGGATC CTATGGTGGC TCGCAAGCAG AAACTTGCAG ATTCTCTTCG CCTGCAGCAG 
CTTTTCCGTG ACATCGAGGA TGAAGAGACC TGGATCAGGG AAAAGGAACC TATTGCAGCC 
TCAACAAACC GAGGCAAGGA CTTAATTGGT GTCCAGAATC TGCTAAAGAA GCACCAGGCT 
TTGCAGGCAG AAATTGCAGG CCATGAGCCT CGCATTAAAG CAGTCACACA AAAAGGAAAT 
GCGATGGTGG AAGAGGGACA TTTTGCTGCT GAAGATGTGA AGATCAAATT GAATGAACTA 
AACCAAAAAT GGGACTCTCT GAAAGCCAAG GCATCTCAAC GGCGACAGGA TCTAGAGGAT 
TCTCTGCAAG CTCAGCAGTA TTTTGCTGAT GCTAATGAGG CAGAATCATG GATGAGGGAA 
AAGGAGCCCA TTGTAGGCAG CACGGATTAT GGAAAGGATG AAGACTCTGC TGAGGCTCTC 
CTGAAGAAGC ATGAAGCTTT GATGTCTGAT CTTTCTGCTT ACGGCAGTAG CATACAGGCA 
TTAAGGGAAC AGGCCCAGTC CTGCAGGCAA CAAGTTGCTC CCACTGATGA TGAAACTGGA 
AAAGAGCTTG TGCTAGCACT CTATGATTAC CAAGAGAAGA GTCCTCGGGA GGTGACTATG 
AAGAAAGGAG ATATTCTAAC CCTGCTCAAC AGCACCAACA AGGACTGGTG GAAGGTTGAA 
GTTAACGATC GTCAGGGCTT TGTACCAGCT GCCTATGTGA AAAAACTAGA TCCTGCCCAG 
TCTGCATCCC GAGAGAATCT TCTAGAAGAG CAAGGCAGCA TAGCATTGCG ACAGGAGCAA 
ATTGACAACC AGTATCATTC CCTTCTTGAG CTGGGAGAAA AACGTAAAGG CATGCTAGAA 
AAAAGCTGCA AGAAGTTTAT GCTTTTCCGT GAGGCTAATG AGCTTCAACA GTGGATCAAT 
GAGAAGGAAG CTGCGCTCAC TAATGAGGAA GTGGGTGCTG ATTTGGAGCA GGTGGAGGTG 
CTACAGAAGA AGTTTGATGA TTTTCAGAAG GATTTAAAAG CTAATGAGTC ACGACTGAAG 
GACATAAACA AGGTTGCAAA TGACCTGGAG TCTGAAGGGC TGATGGCAGA GGAAGTGCAA 
GCAGTAGAGC ACCAGGAAGT CTATGGAATG ATGCCCAGAG ATGAAACTGA TTCTAAGACA 
GCCTCTCCTT GGAAGTCTGC ACGTATGATG GTACACACAG TGGCAACGTT TAACTCAATC 
AAGGAACTGA ATGAACGCTG GAGATCCCTG CAGCAGTTGG CAGAGGAGCG AAGCCAGCTG 
TTGGGCAGCG CTCATGAAGT CCAGAGGTTC CACAGAGATG CTGATGAAAC AAAAGAATGG 
ATAGAAGAGA AGAATCAAGC ATTAAATACC GACAACTATG GACATGACTT GGCCAGTGTG 
CAGGCTCTGC AGCGCAAGCA TGAAGGCTTT GAGAGAGACT TGGCAGCTCT TGGAGACAAG 
GTGAATTCTC TTGGTGAAAC TGCCCAGCGT CTGATCCAGT CACATCCAGA ATCAGCTGAA 
GATCTTCAAG AAAAATGTAC TGAGTTGAAC CAAGCCTGGA GTAGTCTGGG AAAACGTGCT 
GACCAACGCA AAGAGAAGCT TGGAGATTCT CATGACCTGC AGCGTTTCCT CAGTGATTTT 
AGAGACCTTA TGTCTTGGAT CAATGGAATC CGGGGTCTGG TCTCCTCAGA TGAACTTGCA 
AAAGATGTGA CTGGAGCTGA AGCTCTGTTG GAAAGGCATC AGGAGCACCG TACTGAAATA 
GATGCACGGG CTGGCACTTT TCAGGCATTT GAACAGTTTG GACAGCAGCT TCTGGCTCGT 
GGACACTATG CCAGCCCAGA GATTAAGGAG AAACTGGATA TTCTAGATCA AGAACGGACA 
GACCTAGAGA AGGCCTGGGT CCAGCGCAGA ATGATGCTAG ACCAGTGCTT AGAACTACAG 
CTGTTTCATC GGGATTGTGA ACAAGCTGAA AACTGGATGG CTGCCCGAGA GGCGTTCCTA 
AATACAGAAG ACAAAGGAGA CTCCTTAGAC AGCGTGGAGG CACTCATCAA GAAGCATGAA 
GATTTCGATA AAGCAATCAA TGTCCAGGAA GAGAAAATTG CTGTCTTGCA GTCTTTTGCT 
GACCAACTGA TTGCTGCTGA TCATTATGCA AAAGGAGTCA TTGCTAACAG ACGCAATGAG 
GTTCTGGACA GGTGGCGTCG TCTGAAGGCT CAGATGATTG AGAAGAGATC TAAGCTGGGA 
GAATCTCAGA CCCTCCAACA GTTCAGTCGT GATGTTGATG AAATAGAAGC TTGGATCAGT 
GAAAAGCTTC AAACTGCAAG TGATGAGTCA TATAAGGATC CCACAAACAT CCAGCTTTCC 
AAACTGCTGA GCAAGCACCA GAAGCACCAA GCCTTTGAAG CTGAGCTCCA CGCCAACGCA 
GATCGGATTC GTGGAGTCAT TGAAATGGGG AACTCTCTTA TTGAAAGAGG AGCGTGTGCT 
GGCAGCGAGG ATGCTGTGAA GGCTCGGCTG GCTGCCCTGG CTGACCAATG GGAGTTCCTG 
GTCCAGAAGT CATCAGAGAA GAGTCAGAAA CTGAAAGAAG CAAATAAACA GCAGAATTTC 
AATACCGGCA TCAAGGACTT TGATTTCTGG CTTTCAGAGG TGGAAGCTTT GTTGGCATCT 
GAAGACTATG GGAAGGACTT GGCATCAGTG AACAACCTTC TGAAGAAGCA CCAATTACTG 
GAAGCTGATA TATCTGCTCA TGAGGATCGG CTGAAGGACC TGAACAGCCA GGCTGACAGT 
TTGATGACCA GCAGTGCTTT CGATACCTCC CAAGTAAAGG ATAAACGTGA AACTATAAAT 
GGGCGCTTCC AGAGAATCAA GAGCATGGCA GCTGCCCGCC GTGCGAAGCT CAACGAGTCG 
CACCGCTTGC ATCAGTTCTT CCGTGACATG GATGATGAGG AGTCCTGGAT CAAAGAGAAG 
AAACTGTTGG TGAGCTCAGA GGACTATGGC AGAGACCTGA CTGGTGTGCA GAACCTGAGG 
AAGAAACATA AGCGCTTGGA AGCAGAATTA GCTGCCCATG AACCTGCTAT CCAGGGTGTT 
CTAGACACTG GCAAGAAGCT TTCAGATGAT AACACAATTG GGAAGGAGGA GATACAGCAA 
AGACTGGCTC AGTTTGTGGA CCACTGGAAA GAGTTAAAAC AGCTGGCAGC TGCTCGGGGG 
CAGCGTCTGG AGGAGTCCCT GGAGTACCAA CAGTTTGTAG CAAATGTTGA GGAAGAGGAG 
GCATGGATCA ACGAGAAAAT GACATTGGTA GCCAGTGAGG ATTATGGAGA CACACTTGCT 
GCTATCCAGG GCTTGCTGAA GAAGCATGAA GCATTCGAGA CTGACTTTAC TGTCCACAAA 
GACAGAGTGA ATGATGTTTG CGCTAATGGA GAGGATCTCA TTAAAAAGAA TAATCACCAT 
GTGGAGAACA TTACTGCTAA GATGAAGGGC CTCAAGGGCA AGGTATCAGA TCTGGAGAAA 
GCCGCAGCTC AGAGGAAAGC CAAACTGGAT GAGAACTCTG CCTTCCTCCA GTTCAACTGG 
AAAGCAGATG TGGTGGAGTC ATGGATAGGT GAGAAGGAAA ACAGTCTGAA GACAGATGAT 
TATGGCCGTG ACCTCTCCTC TGTGCAAACA CTACTCACCA AACAGGAAAC CTTTGATGCT 
GGACTTCAGG CTTTCCAGCA GGAGGGAATT GCAAACATCA CTGCTCTGAA AGACCAGCTA 
CTGGCAGCCA AACATATCCA ATCAAAGGCC ATTGAGGTTC GTCACGCTTC CTTGATGAAA 
CGCTGGAATC AGCTGCTTGC TAATTCTGCA GCCAGGAAAA AGAAACTCTT GGAGGCTCAG 
GAGCACTTCA GAAAGGTTGA AGATCTCTTC TTGACTTTTG CCAAGAAGGC CTCTGCCTTC 
AACAGTTGGT TTGAGAATGC TGAGGAGGAC CTGACGGATC CCGTGCGCTG CAATTCCCTG 
GAAGAAATCA AAGCACTGCG AGAAGCCCAC GATGCGTTCC GTTCCTCACT TAGCTCTGCC 
CAAGCTGACT TCAACCAGCT GGCAGAGCTT GATCGGCAGA TCAAGAGCTT CCGTGTAGCC 
TCCAACCCGT ACACTTGGTT TACTATGGAG GCTCTTGAAG AAACTTGGAG GAATCTGCAG 
AAAATTATCA AGGAGCGTGA ATTGGAGTTG CAGAAGGAGC AGCGGAGGCA GGAAGAAAAT 
GACAAGTTGC GCCAGGAGTT TGCTCAGCAT GCTAATGCCT TCCACCAGTG GATTCAGGAG 
ACAAGGACTT ACCTGCTAGA TGGCATAGCC TATCGTCGTG TCATTCGTGT CTATCAGTAT 
GAAGTTGGGG ATGATCTATC TGGAAGGTCC TGCATGGTGG AGGAGTCGGG AACACTGGAA 
TCCCAGCTGG AAGCTACTAA ACGCAAGCAC CAGGAGATCC GGGCTATGAG GAGCCAGCTG 
AAGAAGATTG AGGACCTTGG AGCAGCCATG GAAGAGGCAC TTATCTTGGA CAACAAATAC 
ACAGAGCACA GCACCGTGGG GCTGGCGCAG CAGTGGGACC AGCTTGACCA GCTGGGAATG 
AGAATGCAGC ACAATCTGGA ACAGCAGATT CAAGCTCGAA ACACAACTGG AGTCACAGAG 
GAGGCCCTGA AGGAGTTCAG CATGATGTTC AAGCACTTTG ACAAGGACAA ATCTGGACGT 
CTTAATCACC AGGAGTTTAA GTCTTGCTTG CGCTCCCTTG GCTATGACCT GCCCATGGTT 
GAGGAAGGAG AGCCTGACCC CGAATTTGAG TCTATTCTTG ACACTGTTGA TCCCAACAGG 
GATGGCCACG TCTCGCTGCA GGAGTACATG GCGTTCATGA TCAGCAGGGA AACAGAGAAC 
GTGAAATCCA GCGAGGAGAT TGAGAGTGCT TTCCGTGCCC TCAGCTCGGA GGGGAAGCCT 
TACGTGACCA AGGAGGAGCT CTACCAGAAC CTGACCCGGG AGCAGGCCGA CTACTGCATT 
TCTCACATGA AGCCCTACAT GGATGGCAAG GGCAGAGAAC TTCCTTCTGC CTACGACTAC 
ATAGAATTTA CACGTTCACT CTTTGTGAAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015134776.1
CDS	14..7486
Translation	MLSSFRKVKVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSSLRRQKLEDSYRFQFFQRDADELEKWIQEKLQIASDENYKDPSNLQGKLQKHQAFEAEVQANSGAIVKLDETGNQMINEGHFASETIRTRLQELHRLWELLLEKMREKGVKLLQAQKLVQFLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDLAAHEERVNEVNQFAGKLIQEQHPEEELIKSKQDEVNASWQRLKGLAQQRQGKLFGAAEVQRFNRDVDETISWIKEKGQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPINASQIQVKREELIANWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFRSADESGQALLAAGHYASDEVKEKLTILSDERSALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMDDVATRRDALLSRRNALHERAMKRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYASDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTSVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDADNIKKKQEALVARYEALKDPMVARKQKLADSLRLQQLFRDIEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKIKLNELNQKWDSLKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPTDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTNEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVANDLESEGLMAEEVQAVEHQEVYGMMPRDETDSKTASPWKSARMMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAQRLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKEKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLARGHYASPEIKEKLDILDQERTDLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAVLQSFADQLIAADHYAKGVIANRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIEMGNSLIERGACAGSEDAVKARLAALADQWEFLVQKSSEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRETINGRFQRIKSMAAARRAKLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVDHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCANGEDLIKKNNHHVENITAKMKGLKGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHIQSKAIEVRHASLMKRWNQLLANSAARKKKLLEAQEHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGIAYRRVIRVYQYEVGDDLSGRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFESILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCISHMKPYMDGKGRELPSAYDYIEFTRSLFVN

Target ORF information:

RefSeq Version	XM_015279290.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279290.1

ORF Insert Sequence:

ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG 
TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC 
TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT 
GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT 
GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG 
ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAA CTCGTCTACA GGAGCTGCAC 
CGACTATGGG AGTTACTGCT GGAAAAAATG AGAGAGAAGG GAGTGAAGCT GTTGCAAGCA 
CAGAAGCTGG TGCAATTCTT ACGGGAATGT GAAGATGTCA TGGACTGGAT CAATGACAAG 
GAAGCAATAG TGACCTCAGA AGAGCTTGGA CAGGACTTAG AGCATGTTGA GGTTTTGCAG 
AAGAAATTTG AAGAGTTCCA AACAGATCTG GCAGCTCATG AGGAGAGAGT GAATGAAGTG 
AACCAGTTCG CTGGCAAACT TATCCAGGAA CAGCACCCTG AGGAGGAACT TATAAAGTCC 
AAACAGGATG AAGTAAATGC AAGCTGGCAG CGTCTTAAGG GGCTTGCCCA ACAGAGGCAA 
GGAAAACTCT TTGGGGCAGC TGAAGTTCAG CGCTTCAACA GGGATGTGGA TGAAACTATC 
AGCTGGATTA AGGAGAAAGG GCAGTTGATG GCCTCAGATG ACTTTGGCAG AGACTTGGCC 
AGCGTGCAAG CTTTACTACG TAAGCATGAA GGCCTGGAAA GAGATCTTGC AGCTTTGGAG 
GATAAGGTTA AGGCCCTCTG TGCAGAAGCT GACCGTTTGC AGCAGTCTCA CCCTATAAAT 
GCTTCTCAAA TTCAAGTGAA ACGGGAGGAA CTGATTGCCA ACTGGGAGCA GATCCGAACT 
CTGGCAGCAG AGAGGCATGC TCGCCTTAAT GACTCCTACA GGTTGCAGCG CTTTCTTGCA 
GATTTCCGGG ACCTCACTAG CTGGGTAACT GAGATGAAGG CTCTGATAAA TGCTGATGAG 
CTTGCCAATG ATGTGGCTGG AGCAGAAGCC CTTCTAGATA GACATCAGGA ACATAAGGGA 
GAAATTGATG CTCATGAGGA TAGCTTCAGA TCTGCTGATG AGTCTGGGCA GGCTTTGCTT 
GCAGCTGGGC ACTATGCTTC TGATGAAGTT AAAGAAAAGC TGACTATCCT CTCAGATGAA 
AGATCAGCCT TGCTGGAACT ATGGGAGCTT CGCAGGCAAC AGTATGAGCA GTGCATGGAT 
CTGCAGCTTT TCTACAGAGA TACTGAACAA GTTGACAACT GGATGAGCAA ACAAGAAGCT 
TTTCTGCTGA ACGAAGACCT TGGTGATTCT CTGGATAGCG TGGAGGCTCT TCTAAAGAAG 
CATGAAGATT TTGAGAAATC CCTAAGTGCT CAAGAGGAGA AGATCACAGC ACTGGATGAG 
TTTGCTACTA AACTGATTCA AAATAACCAT TATGCCATGG ATGACGTTGC TACGCGCAGA 
GATGCTCTTC TGAGTCGCCG AAATGCTCTT CATGAAAGAG CCATGAAGCG CCGTGCCCAA 
CTGGCAGACT CTTTCCATCT CCAGCAGTTT TTCAGGGACT CTGATGAACT GAAGAGTTGG 
GTTAATGAAA AAATGAAAAC TGCTACCGAT GAGGCTTACA AGGATCCATC CAACTTGCAA 
GGTAAAGTTC AGAAGCATCA GGCTTTTGAA GCAGAGCTTT CTGCTAATCA GAGTCGTATT 
GATGCACTGG AGAAAGCTGG CCAGAAGTTG ATTGATGTCA ATCACTATGC ATCTGATGAG 
GTGGCAGCTC GCATGAATGA AGTCATCAGC TTGTGGAAGA AACTTCTGGA AGCCACCGAG 
CTCAAAGGTA TAAAGCTGCG TGAAGCCAAT CAACAGCAGC AATTTAATCG CAATGTAGAA 
GACATTGAGT TGTGGCTGTA TGAAGTGGAG GGTCACTTGG CTTCTGATGA TTATGGAAAA 
GATCTTACCA GCGTTCAGAA TCTTCAGAAG AAACATGCCC TGCTAGAGGC AGATGTTGCT 
GCCCATCAGG ATCGGATAGA TGGCATTACC ATCCAGGCAC GCCAGTTCCA AGATGCTGGG 
CACTTTGATG CTGACAATAT CAAGAAGAAA CAAGAAGCTT TAGTAGCTCG TTATGAAGCT 
CTGAAGGATC CTATGGTGGC TCGCAAGCAG AAACTTGCAG ATTCTCTTCG CCTGCAGCAG 
CTTTTCCGTG ACATCGAGGA TGAAGAGACC TGGATCAGGG AAAAGGAACC TATTGCAGCC 
TCAACAAACC GAGGCAAGGA CTTAATTGGT GTCCAGAATC TGCTAAAGAA GCACCAGGCT 
TTGCAGGCAG AAATTGCAGG CCATGAGCCT CGCATTAAAG CAGTCACACA AAAAGGAAAT 
GCGATGGTGG AAGAGGGACA TTTTGCTGCT GAAGATGTGA AGATCAAATT GAATGAACTA 
AACCAAAAAT GGGACTCTCT GAAAGCCAAG GCATCTCAAC GGCGACAGGA TCTAGAGGAT 
TCTCTGCAAG CTCAGCAGTA TTTTGCTGAT GCTAATGAGG CAGAATCATG GATGAGGGAA 
AAGGAGCCCA TTGTAGGCAG CACGGATTAT GGAAAGGATG AAGACTCTGC TGAGGCTCTC 
CTGAAGAAGC ATGAAGCTTT GATGTCTGAT CTTTCTGCTT ACGGCAGTAG CATACAGGCA 
TTAAGGGAAC AGGCCCAGTC CTGCAGGCAA CAAGTTGCTC CCACTGATGA TGAAACTGGA 
AAAGAGCTTG TGCTAGCACT CTATGATTAC CAAGAGAAGA GTCCTCGGGA GGTGACTATG 
AAGAAAGGAG ATATTCTAAC CCTGCTCAAC AGCACCAACA AGGACTGGTG GAAGGTTGAA 
GTTAACGATC GTCAGGGCTT TGTACCAGCT GCCTATGTGA AAAAACTAGA TCCTGCCCAG 
TCTGCATCCC GAGAGAATCT TCTAGAAGAG CAAGGCAGCA TAGCATTGCG ACAGGAGCAA 
ATTGACAACC AGTATCATTC CCTTCTTGAG CTGGGAGAAA AACGTAAAGG CATGCTAGAA 
AAAAGCTGCA AGAAGTTTAT GCTTTTCCGT GAGGCTAATG AGCTTCAACA GTGGATCAAT 
GAGAAGGAAG CTGCGCTCAC TAATGAGGAA GTGGGTGCTG ATTTGGAGCA GGTGGAGGTG 
CTACAGAAGA AGTTTGATGA TTTTCAGAAG GATTTAAAAG CTAATGAGTC ACGACTGAAG 
GACATAAACA AGGTTGCAAA TGACCTGGAG TCTGAAGGGC TGATGGCAGA GGAAGTGCAA 
GCAGTAGAGC ACCAGGAAGT CTATGGAATG ATGCCCAGAG ATGAAACTGA TTCTAAGACA 
GCCTCTCCTT GGAAGTCTGC ACGTATGATG GTACACACAG TGGCAACGTT TAACTCAATC 
AAGGAACTGA ATGAACGCTG GAGATCCCTG CAGCAGTTGG CAGAGGAGCG AAGCCAGCTG 
TTGGGCAGCG CTCATGAAGT CCAGAGGTTC CACAGAGATG CTGATGAAAC AAAAGAATGG 
ATAGAAGAGA AGAATCAAGC ATTAAATACC GACAACTATG GACATGACTT GGCCAGTGTG 
CAGGCTCTGC AGCGCAAGCA TGAAGGCTTT GAGAGAGACT TGGCAGCTCT TGGAGACAAG 
GTGAATTCTC TTGGTGAAAC TGCCCAGCGT CTGATCCAGT CACATCCAGA ATCAGCTGAA 
GATCTTCAAG AAAAATGTAC TGAGTTGAAC CAAGCCTGGA GTAGTCTGGG AAAACGTGCT 
GACCAACGCA AAGAGAAGCT TGGAGATTCT CATGACCTGC AGCGTTTCCT CAGTGATTTT 
AGAGACCTTA TGTCTTGGAT CAATGGAATC CGGGGTCTGG TCTCCTCAGA TGAACTTGCA 
AAAGATGTGA CTGGAGCTGA AGCTCTGTTG GAAAGGCATC AGGAGCACCG TACTGAAATA 
GATGCACGGG CTGGCACTTT TCAGGCATTT GAACAGTTTG GACAGCAGCT TCTGGCTCGT 
GGACACTATG CCAGCCCAGA GATTAAGGAG AAACTGGATA TTCTAGATCA AGAACGGACA 
GACCTAGAGA AGGCCTGGGT CCAGCGCAGA ATGATGCTAG ACCAGTGCTT AGAACTACAG 
CTGTTTCATC GGGATTGTGA ACAAGCTGAA AACTGGATGG CTGCCCGAGA GGCGTTCCTA 
AATACAGAAG ACAAAGGAGA CTCCTTAGAC AGCGTGGAGG CACTCATCAA GAAGCATGAA 
GATTTCGATA AAGCAATCAA TGTCCAGGAA GAGAAAATTG CTGTCTTGCA GTCTTTTGCT 
GACCAACTGA TTGCTGCTGA TCATTATGCA AAAGGAGTCA TTGCTAACAG ACGCAATGAG 
GTTCTGGACA GGTGGCGTCG TCTGAAGGCT CAGATGATTG AGAAGAGATC TAAGCTGGGA 
GAATCTCAGA CCCTCCAACA GTTCAGTCGT GATGTTGATG AAATAGAAGC TTGGATCAGT 
GAAAAGCTTC AAACTGCAAG TGATGAGTCA TATAAGGATC CCACAAACAT CCAGCTTTCC 
AAACTGCTGA GCAAGCACCA GAAGCACCAA GCCTTTGAAG CTGAGCTCCA CGCCAACGCA 
GATCGGATTC GTGGAGTCAT TGAAATGGGG AACTCTCTTA TTGAAAGAGG AGCGTGTGCT 
GGCAGCGAGG ATGCTGTGAA GGCTCGGCTG GCTGCCCTGG CTGACCAATG GGAGTTCCTG 
GTCCAGAAGT CATCAGAGAA GAGTCAGAAA CTGAAAGAAG CAAATAAACA GCAGAATTTC 
AATACCGGCA TCAAGGACTT TGATTTCTGG CTTTCAGAGG TGGAAGCTTT GTTGGCATCT 
GAAGACTATG GGAAGGACTT GGCATCAGTG AACAACCTTC TGAAGAAGCA CCAATTACTG 
GAAGCTGATA TATCTGCTCA TGAGGATCGG CTGAAGGACC TGAACAGCCA GGCTGACAGT 
TTGATGACCA GCAGTGCTTT CGATACCTCC CAAGTAAAGG ATAAACGTGA AACTATAAAT 
GGGCGCTTCC AGAGAATCAA GAGCATGGCA GCTGCCCGCC GTGCGAAGCT CAACGAGTCG 
CACCGCTTGC ATCAGTTCTT CCGTGACATG GATGATGAGG AGTCCTGGAT CAAAGAGAAG 
AAACTGTTGG TGAGCTCAGA GGACTATGGC AGAGACCTGA CTGGTGTGCA GAACCTGAGG 
AAGAAACATA AGCGCTTGGA AGCAGAATTA GCTGCCCATG AACCTGCTAT CCAGGGTGTT 
CTAGACACTG GCAAGAAGCT TTCAGATGAT AACACAATTG GGAAGGAGGA GATACAGCAA 
AGACTGGCTC AGTTTGTGGA CCACTGGAAA GAGTTAAAAC AGCTGGCAGC TGCTCGGGGG 
CAGCGTCTGG AGGAGTCCCT GGAGTACCAA CAGTTTGTAG CAAATGTTGA GGAAGAGGAG 
GCATGGATCA ACGAGAAAAT GACATTGGTA GCCAGTGAGG ATTATGGAGA CACACTTGCT 
GCTATCCAGG GCTTGCTGAA GAAGCATGAA GCATTCGAGA CTGACTTTAC TGTCCACAAA 
GACAGAGTGA ATGATGTTTG CGCTAATGGA GAGGATCTCA TTAAAAAGAA TAATCACCAT 
GTGGAGAACA TTACTGCTAA GATGAAGGGC CTCAAGGGCA AGGTATCAGA TCTGGAGAAA 
GCCGCAGCTC AGAGGAAAGC CAAACTGGAT GAGAACTCTG CCTTCCTCCA GTTCAACTGG 
AAAGCAGATG TGGTGGAGTC ATGGATAGGT GAGAAGGAAA ACAGTCTGAA GACAGATGAT 
TATGGCCGTG ACCTCTCCTC TGTGCAAACA CTACTCACCA AACAGGAAAC CTTTGATGCT 
GGACTTCAGG CTTTCCAGCA GGAGGGAATT GCAAACATCA CTGCTCTGAA AGACCAGCTA 
CTGGCAGCCA AACATATCCA ATCAAAGGCC ATTGAGGTTC GTCACGCTTC CTTGATGAAA 
CGCTGGAATC AGCTGCTTGC TAATTCTGCA GCCAGGAAAA AGAAACTCTT GGAGGCTCAG 
GAGCACTTCA GAAAGGTTGA AGATCTCTTC TTGACTTTTG CCAAGAAGGC CTCTGCCTTC 
AACAGTTGGT TTGAGAATGC TGAGGAGGAC CTGACGGATC CCGTGCGCTG CAATTCCCTG 
GAAGAAATCA AAGCACTGCG AGAAGCCCAC GATGCGTTCC GTTCCTCACT TAGCTCTGCC 
CAAGCTGACT TCAACCAGCT GGCAGAGCTT GATCGGCAGA TCAAGAGCTT CCGTGTAGCC 
TCCAACCCGT ACACTTGGTT TACTATGGAG GCTCTTGAAG AAACTTGGAG GAATCTGCAG 
AAAATTATCA AGGAGCGTGA ATTGGAGTTG CAGAAGGAGC AGCGGAGGCA GGAAGAAAAT 
GACAAGTTGC GCCAGGAGTT TGCTCAGCAT GCTAATGCCT TCCACCAGTG GATTCAGGAG 
ACAAGGACTT ACCTGCTAGA TGGCATAGCC TATCGTCGTG TCATTCGTGT CTATCAGTAT 
GAAGTTGGGG ATGATCTATC TGGAAGGTCC TGCATGGTGG AGGAGTCGGG AACACTGGAA 
TCCCAGCTGG AAGCTACTAA ACGCAAGCAC CAGGAGATCC GGGCTATGAG GAGCCAGCTG 
AAGAAGATTG AGGACCTTGG AGCAGCCATG GAAGAGGCAC TTATCTTGGA CAACAAATAC 
ACAGAGCACA GCACCGTGGG GCTGGCGCAG CAGTGGGACC AGCTTGACCA GCTGGGAATG 
AGAATGCAGC ACAATCTGGA ACAGCAGATT CAAGCTCGAA ACACAACTGG AGTCACAGAG 
GAGGCCCTGA AGGAGTTCAG CATGATGTTC AAGCACTTTG ACAAGGACAA ATCTGGACGT 
CTTAATCACC AGGAGTTTAA GTCTTGCTTG CGCTCCCTTG GCTATGACCT GCCCATGGTT 
GAGGAAGGAG AGCCTGACCC CGAATTTGAG TCTATTCTTG ACACTGTTGA TCCCAACAGG 
GATGGCCACG TCTCGCTGCA GGAGTACATG GCGTTCATGA TCAGCAGGGA AACAGAGAAC 
GTGAAATCCA GCGAGGAGAT TGAGAGTGCT TTCCGTGCCC TCAGCTCGGA GGGGAAGCCT 
TACGTGACCA AGGAGGAGCT CTACCAGAAC CTGACCCGGG AGCAGGCCGA CTACTGCATT 
TCTCACATGA AGCCCTACAT GGATGGCAAG GGCAGAGAAC TTCCTTCTGC CTACGACTAC 
ATAGAATTTA CACGTTCACT CTTTGTGAAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_015279291.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015134777.1 Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X10, mRNA.

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CloneID	OGa43317
Clone ID Related Accession (Same CDS sequence)	XM_015279291.2 , XM_015279291.1
Accession Version	XM_015279291.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7470bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015279291.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG 
TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC 
TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT 
GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT 
GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG 
ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAA CTCGTCTACA GGAGCTGCAC 
CGACTATGGG AGTTACTGCT GGAAAAAATG AGAGAGAAGG GAGTGAAGCT GTTGCAAGCA 
CAGAAGCTGG TGCAATTCTT ACGGGAATGT GAAGATGTCA TGGACTGGAT CAATGACAAG 
GAAGCAATAG TGACCTCAGA AGAGCTTGGA CAGGACTTAG AGCATGTTGA GGTTTTGCAG 
AAGAAATTTG AAGAGTTCCA AACAGATCTG GCAGCTCATG AGGAGAGAGT GAATGAAGTG 
AACCAGTTCG CTGGCAAACT TATCCAGGAA CAGCACCCTG AGGAGGAACT TATAAAGTCC 
AAACAGGATG AAGTAAATGC AAGCTGGCAG CGTCTTAAGG GGCTTGCCCA ACAGAGGCAA 
GGAAAACTCT TTGGGGCAGC TGAAGTTCAG CGCTTCAACA GGGATGTGGA TGAAACTATC 
AGCTGGATTA AGGAGAAAGG GCAGTTGATG GCCTCAGATG ACTTTGGCAG AGACTTGGCC 
AGCGTGCAAG CTTTACTACG TAAGCATGAA GGCCTGGAAA GAGATCTTGC AGCTTTGGAG 
GATAAGGTTA AGGCCCTCTG TGCAGAAGCT GACCGTTTGC AGCAGTCTCA CCCTATAAAT 
GCTTCTCAAA TTCAAGTGAA ACGGGAGGAA CTGATTGCCA ACTGGGAGCA GATCCGAACT 
CTGGCAGCAG AGAGGCATGC TCGCCTTAAT GACTCCTACA GGTTGCAGCG CTTTCTTGCA 
GATTTCCGGG ACCTCACTAG CTGGGTAACT GAGATGAAGG CTCTGATAAA TGCTGATGAG 
CTTGCCAATG ATGTGGCTGG AGCAGAAGCC CTTCTAGATA GACATCAGGA ACATAAGGGA 
GAAATTGATG CTCATGAGGA TAGCTTCAGA TCTGCTGATG AGTCTGGGCA GGCTTTGCTT 
GCAGCTGGGC ACTATGCTTC TGATGAAGTT AAAGAAAAGC TGACTATCCT CTCAGATGAA 
AGATCAGCCT TGCTGGAACT ATGGGAGCTT CGCAGGCAAC AGTATGAGCA GTGCATGGAT 
CTGCAGCTTT TCTACAGAGA TACTGAACAA GTTGACAACT GGATGAGCAA ACAAGAAGCT 
TTTCTGCTGA ACGAAGACCT TGGTGATTCT CTGGATAGCG TGGAGGCTCT TCTAAAGAAG 
CATGAAGATT TTGAGAAATC CCTAAGTGCT CAAGAGGAGA AGATCACAGC ACTGGATGAG 
TTTGCTACTA AACTGATTCA AAATAACCAT TATGCCATGG ATGACGTTGC TACGCGCAGA 
GATGCTCTTC TGAGTCGCCG AAATGCTCTT CATGAAAGAG CCATGAAGCG CCGTGCCCAA 
CTGGCAGACT CTTTCCATCT CCAGCAGTTT TTCAGGGACT CTGATGAACT GAAGAGTTGG 
GTTAATGAAA AAATGAAAAC TGCTACCGAT GAGGCTTACA AGGATCCATC CAACTTGCAA 
GGTAAAGTTC AGAAGCATCA GGCTTTTGAA GCAGAGCTTT CTGCTAATCA GAGTCGTATT 
GATGCACTGG AGAAAGCTGG CCAGAAGTTG ATTGATGTCA ATCACTATGC ATCTGATGAG 
GTGGCAGCTC GCATGAATGA AGTCATCAGC TTGTGGAAGA AACTTCTGGA AGCCACCGAG 
CTCAAAGGTA TAAAGCTGCG TGAAGCCAAT CAACAGCAGC AATTTAATCG CAATGTAGAA 
GACATTGAGT TGTGGCTGTA TGAAGTGGAG GGTCACTTGG CTTCTGATGA TTATGGAAAA 
GATCTTACCA GCGTTCAGAA TCTTCAGAAG AAACATGCCC TGCTAGAGGC AGATGTTGCT 
GCCCATCAGG ATCGGATAGA TGGCATTACC ATCCAGGCAC GCCAGTTCCA AGATGCTGGG 
CACTTTGATG CTGACAATAT CAAGAAGAAA CAAGAAGCTT TAGTAGCTCG TTATGAAGCT 
CTGAAGGATC CTATGGTGGC TCGCAAGCAG AAACTTGCAG ATTCTCTTCG CCTGCAGCAG 
CTTTTCCGTG ACATCGAGGA TGAAGAGACC TGGATCAGGG AAAAGGAACC TATTGCAGCC 
TCAACAAACC GAGGCAAGGA CTTAATTGGT GTCCAGAATC TGCTAAAGAA GCACCAGGCT 
TTGCAGGCAG AAATTGCAGG CCATGAGCCT CGCATTAAAG CAGTCACACA AAAAGGAAAT 
GCGATGGTGG AAGAGGGACA TTTTGCTGCT GAAGATGTGA AGATCAAATT GAATGAACTA 
AACCAAAAAT GGGACTCTCT GAAAGCCAAG GCATCTCAAC GGCGACAGGA TCTAGAGGAT 
TCTCTGCAAG CTCAGCAGTA TTTTGCTGAT GCTAATGAGG CAGAATCATG GATGAGGGAA 
AAGGAGCCCA TTGTAGGCAG CACGGATTAT GGAAAGGATG AAGACTCTGC TGAGGCTCTC 
CTGAAGAAGC ATGAAGCTTT GATGTCTGAT CTTTCTGCTT ACGGCAGTAG CATACAGGCA 
TTAAGGGAAC AGGCCCAGTC CTGCAGGCAA CAAGTTGCTC CCACTGATGA TGAAACTGGA 
AAAGAGCTTG TGCTAGCACT CTATGATTAC CAAGAGAAGA GTCCTCGGGA GGTGACTATG 
AAGAAAGGAG ATATTCTAAC CCTGCTCAAC AGCACCAACA AGGACTGGTG GAAGGTTGAA 
GTTAACGATC GTCAGGGCTT TGTACCAGCT GCCTATGTGA AAAAACTAGA TCCTGCCCAG 
TCTGCATCCC GAGAGAATCT TCTAGAAGAG CAAGGCAGCA TAGCATTGCG ACAGGAGCAA 
ATTGACAACC AGACTCTCAT TACTAAGGAA GTCGGCAGTG TATCTCTGCG TATGAAACAG 
GTCGAAGAAC TGTATCATTC CCTTCTTGAG CTGGGAGAAA AACGTAAAGG CATGCTAGAA 
AAAAGCTGCA AGAAGTTTAT GCTTTTCCGT GAGGCTAATG AGCTTCAACA GTGGATCAAT 
GAGAAGGAAG CTGCGCTCAC TAATGAGGAA GTGGGTGCTG ATTTGGAGCA GGTGGAGGTG 
CTACAGAAGA AGTTTGATGA TTTTCAGAAG GATTTAAAAG CTAATGAGTC ACGACTGAAG 
GACATAAACA AGGTTGCAAA TGACCTGGAG TCTGAAGGGC TGATGGCAGA GGAAGTGCAA 
GCAGTAGAGC ACCAGGAAGT CTATGGAATG ATGCCCAGAG ATGAAACTGA TTCTAAGACA 
GCCTCTCCTT GGAAGTCTGC ACGTATGATG GTACACACAG TGGCAACGTT TAACTCAATC 
AAGGAACTGA ATGAACGCTG GAGATCCCTG CAGCAGTTGG CAGAGGAGCG AAGCCAGCTG 
TTGGGCAGCG CTCATGAAGT CCAGAGGTTC CACAGAGATG CTGATGAAAC AAAAGAATGG 
ATAGAAGAGA AGAATCAAGC ATTAAATACC GACAACTATG GACATGACTT GGCCAGTGTG 
CAGGCTCTGC AGCGCAAGCA TGAAGGCTTT GAGAGAGACT TGGCAGCTCT TGGAGACAAG 
GTGAATTCTC TTGGTGAAAC TGCCCAGCGT CTGATCCAGT CACATCCAGA ATCAGCTGAA 
GATCTTCAAG AAAAATGTAC TGAGTTGAAC CAAGCCTGGA GTAGTCTGGG AAAACGTGCT 
GACCAACGCA AAGAGAAGCT TGGAGATTCT CATGACCTGC AGCGTTTCCT CAGTGATTTT 
AGAGACCTTA TGTCTTGGAT CAATGGAATC CGGGGTCTGG TCTCCTCAGA TGAACTTGCA 
AAAGATGTGA CTGGAGCTGA AGCTCTGTTG GAAAGGCATC AGGAGCACCG TACTGAAATA 
GATGCACGGG CTGGCACTTT TCAGGCATTT GAACAGTTTG GACAGCAGCT TCTGGCTCGT 
GGACACTATG CCAGCCCAGA GATTAAGGAG AAACTGGATA TTCTAGATCA AGAACGGACA 
GACCTAGAGA AGGCCTGGGT CCAGCGCAGA ATGATGCTAG ACCAGTGCTT AGAACTACAG 
CTGTTTCATC GGGATTGTGA ACAAGCTGAA AACTGGATGG CTGCCCGAGA GGCGTTCCTA 
AATACAGAAG ACAAAGGAGA CTCCTTAGAC AGCGTGGAGG CACTCATCAA GAAGCATGAA 
GATTTCGATA AAGCAATCAA TGTCCAGGAA GAGAAAATTG CTGTCTTGCA GTCTTTTGCT 
GACCAACTGA TTGCTGCTGA TCATTATGCA AAAGGAGTCA TTGCTAACAG ACGCAATGAG 
GTTCTGGACA GGTGGCGTCG TCTGAAGGCT CAGATGATTG AGAAGAGATC TAAGCTGGGA 
GAATCTCAGA CCCTCCAACA GTTCAGTCGT GATGTTGATG AAATAGAAGC TTGGATCAGT 
GAAAAGCTTC AAACTGCAAG TGATGAGTCA TATAAGGATC CCACAAACAT CCAGCTTTCC 
AAACTGCTGA GCAAGCACCA GAAGCACCAA GCCTTTGAAG CTGAGCTCCA CGCCAACGCA 
GATCGGATTC GTGGAGTCAT TGAAATGGGG AACTCTCTTA TTGAAAGAGG AGCGTGTGCT 
GGCAGCGAGG ATGCTGTGAA GGCTCGGCTG GCTGCCCTGG CTGACCAATG GGAGTTCCTG 
GTCCAGAAGT CATCAGAGAA GAGTCAGAAA CTGAAAGAAG CAAATAAACA GCAGAATTTC 
AATACCGGCA TCAAGGACTT TGATTTCTGG CTTTCAGAGG TGGAAGCTTT GTTGGCATCT 
GAAGACTATG GGAAGGACTT GGCATCAGTG AACAACCTTC TGAAGAAGCA CCAATTACTG 
GAAGCTGATA TATCTGCTCA TGAGGATCGG CTGAAGGACC TGAACAGCCA GGCTGACAGT 
TTGATGACCA GCAGTGCTTT CGATACCTCC CAAGTAAAGG ATAAACGTGA AACTATAAAT 
GGGCGCTTCC AGAGAATCAA GAGCATGGCA GCTGCCCGCC GTGCGAAGCT CAACGAGTCG 
CACCGCTTGC ATCAGTTCTT CCGTGACATG GATGATGAGG AGTCCTGGAT CAAAGAGAAG 
AAACTGTTGG TGAGCTCAGA GGACTATGGC AGAGACCTGA CTGGTGTGCA GAACCTGAGG 
AAGAAACATA AGCGCTTGGA AGCAGAATTA GCTGCCCATG AACCTGCTAT CCAGGGTGTT 
CTAGACACTG GCAAGAAGCT TTCAGATGAT AACACAATTG GGAAGGAGGA GATACAGCAA 
AGACTGGCTC AGTTTGTGGA CCACTGGAAA GAGTTAAAAC AGCTGGCAGC TGCTCGGGGG 
CAGCGTCTGG AGGAGTCCCT GGAGTACCAA CAGTTTGTAG CAAATGTTGA GGAAGAGGAG 
GCATGGATCA ACGAGAAAAT GACATTGGTA GCCAGTGAGG ATTATGGAGA CACACTTGCT 
GCTATCCAGG GCTTGCTGAA GAAGCATGAA GCATTCGAGA CTGACTTTAC TGTCCACAAA 
GACAGAGTGA ATGATGTTTG CGCTAATGGA GAGGATCTCA TTAAAAAGAA TAATCACCAT 
GTGGAGAACA TTACTGCTAA GATGAAGGGC CTCAAGGGCA AGGTATCAGA TCTGGAGAAA 
GCCGCAGCTC AGAGGAAAGC CAAACTGGAT GAGAACTCTG CCTTCCTCCA GTTCAACTGG 
AAAGCAGATG TGGTGGAGTC ATGGATAGGT GAGAAGGAAA ACAGTCTGAA GACAGATGAT 
TATGGCCGTG ACCTCTCCTC TGTGCAAACA CTACTCACCA AACAGGAAAC CTTTGATGCT 
GGACTTCAGG CTTTCCAGCA GGAGGGAATT GCAAACATCA CTGCTCTGAA AGACCAGCTA 
CTGGCAGCCA AACATATCCA ATCAAAGGCC ATTGAGGTTC GTCACGCTTC CTTGATGAAA 
CGCTGGAATC AGCTGCTTGC TAATTCTGCA GCCAGGAAAA AGAAACTCTT GGAGGCTCAG 
GAGCACTTCA GAAAGGTTGA AGATCTCTTC TTGACTTTTG CCAAGAAGGC CTCTGCCTTC 
AACAGTTGGT TTGAGAATGC TGAGGAGGAC CTGACGGATC CCGTGCGCTG CAATTCCCTG 
GAAGAAATCA AAGCACTGCG AGAAGCCCAC GATGCGTTCC GTTCCTCACT TAGCTCTGCC 
CAAGCTGACT TCAACCAGCT GGCAGAGCTT GATCGGCAGA TCAAGAGCTT CCGTGTAGCC 
TCCAACCCGT ACACTTGGTT TACTATGGAG GCTCTTGAAG AAACTTGGAG GAATCTGCAG 
AAAATTATCA AGGAGCGTGA ATTGGAGTTG CAGAAGGAGC AGCGGAGGCA GGAAGAAAAT 
GACAAGTTGC GCCAGGAGTT TGCTCAGCAT GCTAATGCCT TCCACCAGTG GATTCAGGAG 
ACAAGGACTT ACCTGCTAGA TGGGTCCTGC ATGGTGGAGG AGTCGGGAAC ACTGGAATCC 
CAGCTGGAAG CTACTAAACG CAAGCACCAG GAGATCCGGG CTATGAGGAG CCAGCTGAAG 
AAGATTGAGG ACCTTGGAGC AGCCATGGAA GAGGCACTTA TCTTGGACAA CAAATACACA 
GAGCACAGCA CCGTGGGGCT GGCGCAGCAG TGGGACCAGC TTGACCAGCT GGGAATGAGA 
ATGCAGCACA ATCTGGAACA GCAGATTCAA GCTCGAAACA CAACTGGAGT CACAGAGGAG 
GCCCTGAAGG AGTTCAGCAT GATGTTCAAG CACTTTGACA AGGACAAATC TGGACGTCTT 
AATCACCAGG AGTTTAAGTC TTGCTTGCGC TCCCTTGGCT ATGACCTGCC CATGGTTGAG 
GAAGGAGAGC CTGACCCCGA ATTTGAGTCT ATTCTTGACA CTGTTGATCC CAACAGGGAT 
GGCCACGTCT CGCTGCAGGA GTACATGGCG TTCATGATCA GCAGGGAAAC AGAGAACGTG 
AAATCCAGCG AGGAGATTGA GAGTGCTTTC CGTGCCCTCA GCTCGGAGGG GAAGCCTTAC 
GTGACCAAGG AGGAGCTCTA CCAGAACCTG ACCCGGGAGC AGGCCGACTA CTGCATTTCT 
CACATGAAGC CCTACATGGA TGGCAAGGGC AGAGAACTTC CTTCTGCCTA CGACTACATA 
GAATTTACAC GTTCACTCTT TGTGAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015134777.1
CDS	9..7478
Translation	MLSSFRKVKVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSSLRRQKLEDSYRFQFFQRDADELEKWIQEKLQIASDENYKDPSNLQGKLQKHQAFEAEVQANSGAIVKLDETGNQMINEGHFASETIRTRLQELHRLWELLLEKMREKGVKLLQAQKLVQFLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDLAAHEERVNEVNQFAGKLIQEQHPEEELIKSKQDEVNASWQRLKGLAQQRQGKLFGAAEVQRFNRDVDETISWIKEKGQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPINASQIQVKREELIANWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFRSADESGQALLAAGHYASDEVKEKLTILSDERSALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMDDVATRRDALLSRRNALHERAMKRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYASDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTSVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDADNIKKKQEALVARYEALKDPMVARKQKLADSLRLQQLFRDIEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKIKLNELNQKWDSLKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPTDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTLITKEVGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTNEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVANDLESEGLMAEEVQAVEHQEVYGMMPRDETDSKTASPWKSARMMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAQRLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKEKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLARGHYASPEIKEKLDILDQERTDLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAVLQSFADQLIAADHYAKGVIANRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIEMGNSLIERGACAGSEDAVKARLAALADQWEFLVQKSSEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRETINGRFQRIKSMAAARRAKLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVDHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCANGEDLIKKNNHHVENITAKMKGLKGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHIQSKAIEVRHASLMKRWNQLLANSAARKKKLLEAQEHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFESILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCISHMKPYMDGKGRELPSAYDYIEFTRSLFVN

Target ORF information:

RefSeq Version	XM_015279291.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279291.2

ORF Insert Sequence:

ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG 
TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC 
TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT 
GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT 
GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG 
ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAA CTCGTCTACA GGAGCTGCAC 
CGACTATGGG AGTTACTGCT GGAAAAAATG AGAGAGAAGG GAGTGAAGCT GTTGCAAGCA 
CAGAAGCTGG TGCAATTCTT ACGGGAATGT GAAGATGTCA TGGACTGGAT CAATGACAAG 
GAAGCAATAG TGACCTCAGA AGAGCTTGGA CAGGACTTAG AGCATGTTGA GGTTTTGCAG 
AAGAAATTTG AAGAGTTCCA AACAGATCTG GCAGCTCATG AGGAGAGAGT GAATGAAGTG 
AACCAGTTCG CTGGCAAACT TATCCAGGAA CAGCACCCTG AGGAGGAACT TATAAAGTCC 
AAACAGGATG AAGTAAATGC AAGCTGGCAG CGTCTTAAGG GGCTTGCCCA ACAGAGGCAA 
GGAAAACTCT TTGGGGCAGC TGAAGTTCAG CGCTTCAACA GGGATGTGGA TGAAACTATC 
AGCTGGATTA AGGAGAAAGG GCAGTTGATG GCCTCAGATG ACTTTGGCAG AGACTTGGCC 
AGCGTGCAAG CTTTACTACG TAAGCATGAA GGCCTGGAAA GAGATCTTGC AGCTTTGGAG 
GATAAGGTTA AGGCCCTCTG TGCAGAAGCT GACCGTTTGC AGCAGTCTCA CCCTATAAAT 
GCTTCTCAAA TTCAAGTGAA ACGGGAGGAA CTGATTGCCA ACTGGGAGCA GATCCGAACT 
CTGGCAGCAG AGAGGCATGC TCGCCTTAAT GACTCCTACA GGTTGCAGCG CTTTCTTGCA 
GATTTCCGGG ACCTCACTAG CTGGGTAACT GAGATGAAGG CTCTGATAAA TGCTGATGAG 
CTTGCCAATG ATGTGGCTGG AGCAGAAGCC CTTCTAGATA GACATCAGGA ACATAAGGGA 
GAAATTGATG CTCATGAGGA TAGCTTCAGA TCTGCTGATG AGTCTGGGCA GGCTTTGCTT 
GCAGCTGGGC ACTATGCTTC TGATGAAGTT AAAGAAAAGC TGACTATCCT CTCAGATGAA 
AGATCAGCCT TGCTGGAACT ATGGGAGCTT CGCAGGCAAC AGTATGAGCA GTGCATGGAT 
CTGCAGCTTT TCTACAGAGA TACTGAACAA GTTGACAACT GGATGAGCAA ACAAGAAGCT 
TTTCTGCTGA ACGAAGACCT TGGTGATTCT CTGGATAGCG TGGAGGCTCT TCTAAAGAAG 
CATGAAGATT TTGAGAAATC CCTAAGTGCT CAAGAGGAGA AGATCACAGC ACTGGATGAG 
TTTGCTACTA AACTGATTCA AAATAACCAT TATGCCATGG ATGACGTTGC TACGCGCAGA 
GATGCTCTTC TGAGTCGCCG AAATGCTCTT CATGAAAGAG CCATGAAGCG CCGTGCCCAA 
CTGGCAGACT CTTTCCATCT CCAGCAGTTT TTCAGGGACT CTGATGAACT GAAGAGTTGG 
GTTAATGAAA AAATGAAAAC TGCTACCGAT GAGGCTTACA AGGATCCATC CAACTTGCAA 
GGTAAAGTTC AGAAGCATCA GGCTTTTGAA GCAGAGCTTT CTGCTAATCA GAGTCGTATT 
GATGCACTGG AGAAAGCTGG CCAGAAGTTG ATTGATGTCA ATCACTATGC ATCTGATGAG 
GTGGCAGCTC GCATGAATGA AGTCATCAGC TTGTGGAAGA AACTTCTGGA AGCCACCGAG 
CTCAAAGGTA TAAAGCTGCG TGAAGCCAAT CAACAGCAGC AATTTAATCG CAATGTAGAA 
GACATTGAGT TGTGGCTGTA TGAAGTGGAG GGTCACTTGG CTTCTGATGA TTATGGAAAA 
GATCTTACCA GCGTTCAGAA TCTTCAGAAG AAACATGCCC TGCTAGAGGC AGATGTTGCT 
GCCCATCAGG ATCGGATAGA TGGCATTACC ATCCAGGCAC GCCAGTTCCA AGATGCTGGG 
CACTTTGATG CTGACAATAT CAAGAAGAAA CAAGAAGCTT TAGTAGCTCG TTATGAAGCT 
CTGAAGGATC CTATGGTGGC TCGCAAGCAG AAACTTGCAG ATTCTCTTCG CCTGCAGCAG 
CTTTTCCGTG ACATCGAGGA TGAAGAGACC TGGATCAGGG AAAAGGAACC TATTGCAGCC 
TCAACAAACC GAGGCAAGGA CTTAATTGGT GTCCAGAATC TGCTAAAGAA GCACCAGGCT 
TTGCAGGCAG AAATTGCAGG CCATGAGCCT CGCATTAAAG CAGTCACACA AAAAGGAAAT 
GCGATGGTGG AAGAGGGACA TTTTGCTGCT GAAGATGTGA AGATCAAATT GAATGAACTA 
AACCAAAAAT GGGACTCTCT GAAAGCCAAG GCATCTCAAC GGCGACAGGA TCTAGAGGAT 
TCTCTGCAAG CTCAGCAGTA TTTTGCTGAT GCTAATGAGG CAGAATCATG GATGAGGGAA 
AAGGAGCCCA TTGTAGGCAG CACGGATTAT GGAAAGGATG AAGACTCTGC TGAGGCTCTC 
CTGAAGAAGC ATGAAGCTTT GATGTCTGAT CTTTCTGCTT ACGGCAGTAG CATACAGGCA 
TTAAGGGAAC AGGCCCAGTC CTGCAGGCAA CAAGTTGCTC CCACTGATGA TGAAACTGGA 
AAAGAGCTTG TGCTAGCACT CTATGATTAC CAAGAGAAGA GTCCTCGGGA GGTGACTATG 
AAGAAAGGAG ATATTCTAAC CCTGCTCAAC AGCACCAACA AGGACTGGTG GAAGGTTGAA 
GTTAACGATC GTCAGGGCTT TGTACCAGCT GCCTATGTGA AAAAACTAGA TCCTGCCCAG 
TCTGCATCCC GAGAGAATCT TCTAGAAGAG CAAGGCAGCA TAGCATTGCG ACAGGAGCAA 
ATTGACAACC AGACTCTCAT TACTAAGGAA GTCGGCAGTG TATCTCTGCG TATGAAACAG 
GTCGAAGAAC TGTATCATTC CCTTCTTGAG CTGGGAGAAA AACGTAAAGG CATGCTAGAA 
AAAAGCTGCA AGAAGTTTAT GCTTTTCCGT GAGGCTAATG AGCTTCAACA GTGGATCAAT 
GAGAAGGAAG CTGCGCTCAC TAATGAGGAA GTGGGTGCTG ATTTGGAGCA GGTGGAGGTG 
CTACAGAAGA AGTTTGATGA TTTTCAGAAG GATTTAAAAG CTAATGAGTC ACGACTGAAG 
GACATAAACA AGGTTGCAAA TGACCTGGAG TCTGAAGGGC TGATGGCAGA GGAAGTGCAA 
GCAGTAGAGC ACCAGGAAGT CTATGGAATG ATGCCCAGAG ATGAAACTGA TTCTAAGACA 
GCCTCTCCTT GGAAGTCTGC ACGTATGATG GTACACACAG TGGCAACGTT TAACTCAATC 
AAGGAACTGA ATGAACGCTG GAGATCCCTG CAGCAGTTGG CAGAGGAGCG AAGCCAGCTG 
TTGGGCAGCG CTCATGAAGT CCAGAGGTTC CACAGAGATG CTGATGAAAC AAAAGAATGG 
ATAGAAGAGA AGAATCAAGC ATTAAATACC GACAACTATG GACATGACTT GGCCAGTGTG 
CAGGCTCTGC AGCGCAAGCA TGAAGGCTTT GAGAGAGACT TGGCAGCTCT TGGAGACAAG 
GTGAATTCTC TTGGTGAAAC TGCCCAGCGT CTGATCCAGT CACATCCAGA ATCAGCTGAA 
GATCTTCAAG AAAAATGTAC TGAGTTGAAC CAAGCCTGGA GTAGTCTGGG AAAACGTGCT 
GACCAACGCA AAGAGAAGCT TGGAGATTCT CATGACCTGC AGCGTTTCCT CAGTGATTTT 
AGAGACCTTA TGTCTTGGAT CAATGGAATC CGGGGTCTGG TCTCCTCAGA TGAACTTGCA 
AAAGATGTGA CTGGAGCTGA AGCTCTGTTG GAAAGGCATC AGGAGCACCG TACTGAAATA 
GATGCACGGG CTGGCACTTT TCAGGCATTT GAACAGTTTG GACAGCAGCT TCTGGCTCGT 
GGACACTATG CCAGCCCAGA GATTAAGGAG AAACTGGATA TTCTAGATCA AGAACGGACA 
GACCTAGAGA AGGCCTGGGT CCAGCGCAGA ATGATGCTAG ACCAGTGCTT AGAACTACAG 
CTGTTTCATC GGGATTGTGA ACAAGCTGAA AACTGGATGG CTGCCCGAGA GGCGTTCCTA 
AATACAGAAG ACAAAGGAGA CTCCTTAGAC AGCGTGGAGG CACTCATCAA GAAGCATGAA 
GATTTCGATA AAGCAATCAA TGTCCAGGAA GAGAAAATTG CTGTCTTGCA GTCTTTTGCT 
GACCAACTGA TTGCTGCTGA TCATTATGCA AAAGGAGTCA TTGCTAACAG ACGCAATGAG 
GTTCTGGACA GGTGGCGTCG TCTGAAGGCT CAGATGATTG AGAAGAGATC TAAGCTGGGA 
GAATCTCAGA CCCTCCAACA GTTCAGTCGT GATGTTGATG AAATAGAAGC TTGGATCAGT 
GAAAAGCTTC AAACTGCAAG TGATGAGTCA TATAAGGATC CCACAAACAT CCAGCTTTCC 
AAACTGCTGA GCAAGCACCA GAAGCACCAA GCCTTTGAAG CTGAGCTCCA CGCCAACGCA 
GATCGGATTC GTGGAGTCAT TGAAATGGGG AACTCTCTTA TTGAAAGAGG AGCGTGTGCT 
GGCAGCGAGG ATGCTGTGAA GGCTCGGCTG GCTGCCCTGG CTGACCAATG GGAGTTCCTG 
GTCCAGAAGT CATCAGAGAA GAGTCAGAAA CTGAAAGAAG CAAATAAACA GCAGAATTTC 
AATACCGGCA TCAAGGACTT TGATTTCTGG CTTTCAGAGG TGGAAGCTTT GTTGGCATCT 
GAAGACTATG GGAAGGACTT GGCATCAGTG AACAACCTTC TGAAGAAGCA CCAATTACTG 
GAAGCTGATA TATCTGCTCA TGAGGATCGG CTGAAGGACC TGAACAGCCA GGCTGACAGT 
TTGATGACCA GCAGTGCTTT CGATACCTCC CAAGTAAAGG ATAAACGTGA AACTATAAAT 
GGGCGCTTCC AGAGAATCAA GAGCATGGCA GCTGCCCGCC GTGCGAAGCT CAACGAGTCG 
CACCGCTTGC ATCAGTTCTT CCGTGACATG GATGATGAGG AGTCCTGGAT CAAAGAGAAG 
AAACTGTTGG TGAGCTCAGA GGACTATGGC AGAGACCTGA CTGGTGTGCA GAACCTGAGG 
AAGAAACATA AGCGCTTGGA AGCAGAATTA GCTGCCCATG AACCTGCTAT CCAGGGTGTT 
CTAGACACTG GCAAGAAGCT TTCAGATGAT AACACAATTG GGAAGGAGGA GATACAGCAA 
AGACTGGCTC AGTTTGTGGA CCACTGGAAA GAGTTAAAAC AGCTGGCAGC TGCTCGGGGG 
CAGCGTCTGG AGGAGTCCCT GGAGTACCAA CAGTTTGTAG CAAATGTTGA GGAAGAGGAG 
GCATGGATCA ACGAGAAAAT GACATTGGTA GCCAGTGAGG ATTATGGAGA CACACTTGCT 
GCTATCCAGG GCTTGCTGAA GAAGCATGAA GCATTCGAGA CTGACTTTAC TGTCCACAAA 
GACAGAGTGA ATGATGTTTG CGCTAATGGA GAGGATCTCA TTAAAAAGAA TAATCACCAT 
GTGGAGAACA TTACTGCTAA GATGAAGGGC CTCAAGGGCA AGGTATCAGA TCTGGAGAAA 
GCCGCAGCTC AGAGGAAAGC CAAACTGGAT GAGAACTCTG CCTTCCTCCA GTTCAACTGG 
AAAGCAGATG TGGTGGAGTC ATGGATAGGT GAGAAGGAAA ACAGTCTGAA GACAGATGAT 
TATGGCCGTG ACCTCTCCTC TGTGCAAACA CTACTCACCA AACAGGAAAC CTTTGATGCT 
GGACTTCAGG CTTTCCAGCA GGAGGGAATT GCAAACATCA CTGCTCTGAA AGACCAGCTA 
CTGGCAGCCA AACATATCCA ATCAAAGGCC ATTGAGGTTC GTCACGCTTC CTTGATGAAA 
CGCTGGAATC AGCTGCTTGC TAATTCTGCA GCCAGGAAAA AGAAACTCTT GGAGGCTCAG 
GAGCACTTCA GAAAGGTTGA AGATCTCTTC TTGACTTTTG CCAAGAAGGC CTCTGCCTTC 
AACAGTTGGT TTGAGAATGC TGAGGAGGAC CTGACGGATC CCGTGCGCTG CAATTCCCTG 
GAAGAAATCA AAGCACTGCG AGAAGCCCAC GATGCGTTCC GTTCCTCACT TAGCTCTGCC 
CAAGCTGACT TCAACCAGCT GGCAGAGCTT GATCGGCAGA TCAAGAGCTT CCGTGTAGCC 
TCCAACCCGT ACACTTGGTT TACTATGGAG GCTCTTGAAG AAACTTGGAG GAATCTGCAG 
AAAATTATCA AGGAGCGTGA ATTGGAGTTG CAGAAGGAGC AGCGGAGGCA GGAAGAAAAT 
GACAAGTTGC GCCAGGAGTT TGCTCAGCAT GCTAATGCCT TCCACCAGTG GATTCAGGAG 
ACAAGGACTT ACCTGCTAGA TGGGTCCTGC ATGGTGGAGG AGTCGGGAAC ACTGGAATCC 
CAGCTGGAAG CTACTAAACG CAAGCACCAG GAGATCCGGG CTATGAGGAG CCAGCTGAAG 
AAGATTGAGG ACCTTGGAGC AGCCATGGAA GAGGCACTTA TCTTGGACAA CAAATACACA 
GAGCACAGCA CCGTGGGGCT GGCGCAGCAG TGGGACCAGC TTGACCAGCT GGGAATGAGA 
ATGCAGCACA ATCTGGAACA GCAGATTCAA GCTCGAAACA CAACTGGAGT CACAGAGGAG 
GCCCTGAAGG AGTTCAGCAT GATGTTCAAG CACTTTGACA AGGACAAATC TGGACGTCTT 
AATCACCAGG AGTTTAAGTC TTGCTTGCGC TCCCTTGGCT ATGACCTGCC CATGGTTGAG 
GAAGGAGAGC CTGACCCCGA ATTTGAGTCT ATTCTTGACA CTGTTGATCC CAACAGGGAT 
GGCCACGTCT CGCTGCAGGA GTACATGGCG TTCATGATCA GCAGGGAAAC AGAGAACGTG 
AAATCCAGCG AGGAGATTGA GAGTGCTTTC CGTGCCCTCA GCTCGGAGGG GAAGCCTTAC 
GTGACCAAGG AGGAGCTCTA CCAGAACCTG ACCCGGGAGC AGGCCGACTA CTGCATTTCT 
CACATGAAGC CCTACATGGA TGGCAAGGGC AGAGAACTTC CTTCTGCCTA CGACTACATA 
GAATTTACAC GTTCACTCTT TGTGAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_015279291.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015134777.1 Gallus gallus spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X10, mRNA.

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CloneID	OGa43317
Clone ID Related Accession (Same CDS sequence)	XM_015279291.2 , XM_015279291.1
Accession Version	XM_015279291.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7470bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG 
TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC 
TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT 
GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT 
GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG 
ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAA CTCGTCTACA GGAGCTGCAC 
CGACTATGGG AGTTACTGCT GGAAAAAATG AGAGAGAAGG GAGTGAAGCT GTTGCAAGCA 
CAGAAGCTGG TGCAATTCTT ACGGGAATGT GAAGATGTCA TGGACTGGAT CAATGACAAG 
GAAGCAATAG TGACCTCAGA AGAGCTTGGA CAGGACTTAG AGCATGTTGA GGTTTTGCAG 
AAGAAATTTG AAGAGTTCCA AACAGATCTG GCAGCTCATG AGGAGAGAGT GAATGAAGTG 
AACCAGTTCG CTGGCAAACT TATCCAGGAA CAGCACCCTG AGGAGGAACT TATAAAGTCC 
AAACAGGATG AAGTAAATGC AAGCTGGCAG CGTCTTAAGG GGCTTGCCCA ACAGAGGCAA 
GGAAAACTCT TTGGGGCAGC TGAAGTTCAG CGCTTCAACA GGGATGTGGA TGAAACTATC 
AGCTGGATTA AGGAGAAAGG GCAGTTGATG GCCTCAGATG ACTTTGGCAG AGACTTGGCC 
AGCGTGCAAG CTTTACTACG TAAGCATGAA GGCCTGGAAA GAGATCTTGC AGCTTTGGAG 
GATAAGGTTA AGGCCCTCTG TGCAGAAGCT GACCGTTTGC AGCAGTCTCA CCCTATAAAT 
GCTTCTCAAA TTCAAGTGAA ACGGGAGGAA CTGATTGCCA ACTGGGAGCA GATCCGAACT 
CTGGCAGCAG AGAGGCATGC TCGCCTTAAT GACTCCTACA GGTTGCAGCG CTTTCTTGCA 
GATTTCCGGG ACCTCACTAG CTGGGTAACT GAGATGAAGG CTCTGATAAA TGCTGATGAG 
CTTGCCAATG ATGTGGCTGG AGCAGAAGCC CTTCTAGATA GACATCAGGA ACATAAGGGA 
GAAATTGATG CTCATGAGGA TAGCTTCAGA TCTGCTGATG AGTCTGGGCA GGCTTTGCTT 
GCAGCTGGGC ACTATGCTTC TGATGAAGTT AAAGAAAAGC TGACTATCCT CTCAGATGAA 
AGATCAGCCT TGCTGGAACT ATGGGAGCTT CGCAGGCAAC AGTATGAGCA GTGCATGGAT 
CTGCAGCTTT TCTACAGAGA TACTGAACAA GTTGACAACT GGATGAGCAA ACAAGAAGCT 
TTTCTGCTGA ACGAAGACCT TGGTGATTCT CTGGATAGCG TGGAGGCTCT TCTAAAGAAG 
CATGAAGATT TTGAGAAATC CCTAAGTGCT CAAGAGGAGA AGATCACAGC ACTGGATGAG 
TTTGCTACTA AACTGATTCA AAATAACCAT TATGCCATGG ATGACGTTGC TACGCGCAGA 
GATGCTCTTC TGAGTCGCCG AAATGCTCTT CATGAAAGAG CCATGAAGCG CCGTGCCCAA 
CTGGCAGACT CTTTCCATCT CCAGCAGTTT TTCAGGGACT CTGATGAACT GAAGAGTTGG 
GTTAATGAAA AAATGAAAAC TGCTACCGAT GAGGCTTACA AGGATCCATC CAACTTGCAA 
GGTAAAGTTC AGAAGCATCA GGCTTTTGAA GCAGAGCTTT CTGCTAATCA GAGTCGTATT 
GATGCACTGG AGAAAGCTGG CCAGAAGTTG ATTGATGTCA ATCACTATGC ATCTGATGAG 
GTGGCAGCTC GCATGAATGA AGTCATCAGC TTGTGGAAGA AACTTCTGGA AGCCACCGAG 
CTCAAAGGTA TAAAGCTGCG TGAAGCCAAT CAACAGCAGC AATTTAATCG CAATGTAGAA 
GACATTGAGT TGTGGCTGTA TGAAGTGGAG GGTCACTTGG CTTCTGATGA TTATGGAAAA 
GATCTTACCA GCGTTCAGAA TCTTCAGAAG AAACATGCCC TGCTAGAGGC AGATGTTGCT 
GCCCATCAGG ATCGGATAGA TGGCATTACC ATCCAGGCAC GCCAGTTCCA AGATGCTGGG 
CACTTTGATG CTGACAATAT CAAGAAGAAA CAAGAAGCTT TAGTAGCTCG TTATGAAGCT 
CTGAAGGATC CTATGGTGGC TCGCAAGCAG AAACTTGCAG ATTCTCTTCG CCTGCAGCAG 
CTTTTCCGTG ACATCGAGGA TGAAGAGACC TGGATCAGGG AAAAGGAACC TATTGCAGCC 
TCAACAAACC GAGGCAAGGA CTTAATTGGT GTCCAGAATC TGCTAAAGAA GCACCAGGCT 
TTGCAGGCAG AAATTGCAGG CCATGAGCCT CGCATTAAAG CAGTCACACA AAAAGGAAAT 
GCGATGGTGG AAGAGGGACA TTTTGCTGCT GAAGATGTGA AGATCAAATT GAATGAACTA 
AACCAAAAAT GGGACTCTCT GAAAGCCAAG GCATCTCAAC GGCGACAGGA TCTAGAGGAT 
TCTCTGCAAG CTCAGCAGTA TTTTGCTGAT GCTAATGAGG CAGAATCATG GATGAGGGAA 
AAGGAGCCCA TTGTAGGCAG CACGGATTAT GGAAAGGATG AAGACTCTGC TGAGGCTCTC 
CTGAAGAAGC ATGAAGCTTT GATGTCTGAT CTTTCTGCTT ACGGCAGTAG CATACAGGCA 
TTAAGGGAAC AGGCCCAGTC CTGCAGGCAA CAAGTTGCTC CCACTGATGA TGAAACTGGA 
AAAGAGCTTG TGCTAGCACT CTATGATTAC CAAGAGAAGA GTCCTCGGGA GGTGACTATG 
AAGAAAGGAG ATATTCTAAC CCTGCTCAAC AGCACCAACA AGGACTGGTG GAAGGTTGAA 
GTTAACGATC GTCAGGGCTT TGTACCAGCT GCCTATGTGA AAAAACTAGA TCCTGCCCAG 
TCTGCATCCC GAGAGAATCT TCTAGAAGAG CAAGGCAGCA TAGCATTGCG ACAGGAGCAA 
ATTGACAACC AGACTCTCAT TACTAAGGAA GTCGGCAGTG TATCTCTGCG TATGAAACAG 
GTCGAAGAAC TGTATCATTC CCTTCTTGAG CTGGGAGAAA AACGTAAAGG CATGCTAGAA 
AAAAGCTGCA AGAAGTTTAT GCTTTTCCGT GAGGCTAATG AGCTTCAACA GTGGATCAAT 
GAGAAGGAAG CTGCGCTCAC TAATGAGGAA GTGGGTGCTG ATTTGGAGCA GGTGGAGGTG 
CTACAGAAGA AGTTTGATGA TTTTCAGAAG GATTTAAAAG CTAATGAGTC ACGACTGAAG 
GACATAAACA AGGTTGCAAA TGACCTGGAG TCTGAAGGGC TGATGGCAGA GGAAGTGCAA 
GCAGTAGAGC ACCAGGAAGT CTATGGAATG ATGCCCAGAG ATGAAACTGA TTCTAAGACA 
GCCTCTCCTT GGAAGTCTGC ACGTATGATG GTACACACAG TGGCAACGTT TAACTCAATC 
AAGGAACTGA ATGAACGCTG GAGATCCCTG CAGCAGTTGG CAGAGGAGCG AAGCCAGCTG 
TTGGGCAGCG CTCATGAAGT CCAGAGGTTC CACAGAGATG CTGATGAAAC AAAAGAATGG 
ATAGAAGAGA AGAATCAAGC ATTAAATACC GACAACTATG GACATGACTT GGCCAGTGTG 
CAGGCTCTGC AGCGCAAGCA TGAAGGCTTT GAGAGAGACT TGGCAGCTCT TGGAGACAAG 
GTGAATTCTC TTGGTGAAAC TGCCCAGCGT CTGATCCAGT CACATCCAGA ATCAGCTGAA 
GATCTTCAAG AAAAATGTAC TGAGTTGAAC CAAGCCTGGA GTAGTCTGGG AAAACGTGCT 
GACCAACGCA AAGAGAAGCT TGGAGATTCT CATGACCTGC AGCGTTTCCT CAGTGATTTT 
AGAGACCTTA TGTCTTGGAT CAATGGAATC CGGGGTCTGG TCTCCTCAGA TGAACTTGCA 
AAAGATGTGA CTGGAGCTGA AGCTCTGTTG GAAAGGCATC AGGAGCACCG TACTGAAATA 
GATGCACGGG CTGGCACTTT TCAGGCATTT GAACAGTTTG GACAGCAGCT TCTGGCTCGT 
GGACACTATG CCAGCCCAGA GATTAAGGAG AAACTGGATA TTCTAGATCA AGAACGGACA 
GACCTAGAGA AGGCCTGGGT CCAGCGCAGA ATGATGCTAG ACCAGTGCTT AGAACTACAG 
CTGTTTCATC GGGATTGTGA ACAAGCTGAA AACTGGATGG CTGCCCGAGA GGCGTTCCTA 
AATACAGAAG ACAAAGGAGA CTCCTTAGAC AGCGTGGAGG CACTCATCAA GAAGCATGAA 
GATTTCGATA AAGCAATCAA TGTCCAGGAA GAGAAAATTG CTGTCTTGCA GTCTTTTGCT 
GACCAACTGA TTGCTGCTGA TCATTATGCA AAAGGAGTCA TTGCTAACAG ACGCAATGAG 
GTTCTGGACA GGTGGCGTCG TCTGAAGGCT CAGATGATTG AGAAGAGATC TAAGCTGGGA 
GAATCTCAGA CCCTCCAACA GTTCAGTCGT GATGTTGATG AAATAGAAGC TTGGATCAGT 
GAAAAGCTTC AAACTGCAAG TGATGAGTCA TATAAGGATC CCACAAACAT CCAGCTTTCC 
AAACTGCTGA GCAAGCACCA GAAGCACCAA GCCTTTGAAG CTGAGCTCCA CGCCAACGCA 
GATCGGATTC GTGGAGTCAT TGAAATGGGG AACTCTCTTA TTGAAAGAGG AGCGTGTGCT 
GGCAGCGAGG ATGCTGTGAA GGCTCGGCTG GCTGCCCTGG CTGACCAATG GGAGTTCCTG 
GTCCAGAAGT CATCAGAGAA GAGTCAGAAA CTGAAAGAAG CAAATAAACA GCAGAATTTC 
AATACCGGCA TCAAGGACTT TGATTTCTGG CTTTCAGAGG TGGAAGCTTT GTTGGCATCT 
GAAGACTATG GGAAGGACTT GGCATCAGTG AACAACCTTC TGAAGAAGCA CCAATTACTG 
GAAGCTGATA TATCTGCTCA TGAGGATCGG CTGAAGGACC TGAACAGCCA GGCTGACAGT 
TTGATGACCA GCAGTGCTTT CGATACCTCC CAAGTAAAGG ATAAACGTGA AACTATAAAT 
GGGCGCTTCC AGAGAATCAA GAGCATGGCA GCTGCCCGCC GTGCGAAGCT CAACGAGTCG 
CACCGCTTGC ATCAGTTCTT CCGTGACATG GATGATGAGG AGTCCTGGAT CAAAGAGAAG 
AAACTGTTGG TGAGCTCAGA GGACTATGGC AGAGACCTGA CTGGTGTGCA GAACCTGAGG 
AAGAAACATA AGCGCTTGGA AGCAGAATTA GCTGCCCATG AACCTGCTAT CCAGGGTGTT 
CTAGACACTG GCAAGAAGCT TTCAGATGAT AACACAATTG GGAAGGAGGA GATACAGCAA 
AGACTGGCTC AGTTTGTGGA CCACTGGAAA GAGTTAAAAC AGCTGGCAGC TGCTCGGGGG 
CAGCGTCTGG AGGAGTCCCT GGAGTACCAA CAGTTTGTAG CAAATGTTGA GGAAGAGGAG 
GCATGGATCA ACGAGAAAAT GACATTGGTA GCCAGTGAGG ATTATGGAGA CACACTTGCT 
GCTATCCAGG GCTTGCTGAA GAAGCATGAA GCATTCGAGA CTGACTTTAC TGTCCACAAA 
GACAGAGTGA ATGATGTTTG CGCTAATGGA GAGGATCTCA TTAAAAAGAA TAATCACCAT 
GTGGAGAACA TTACTGCTAA GATGAAGGGC CTCAAGGGCA AGGTATCAGA TCTGGAGAAA 
GCCGCAGCTC AGAGGAAAGC CAAACTGGAT GAGAACTCTG CCTTCCTCCA GTTCAACTGG 
AAAGCAGATG TGGTGGAGTC ATGGATAGGT GAGAAGGAAA ACAGTCTGAA GACAGATGAT 
TATGGCCGTG ACCTCTCCTC TGTGCAAACA CTACTCACCA AACAGGAAAC CTTTGATGCT 
GGACTTCAGG CTTTCCAGCA GGAGGGAATT GCAAACATCA CTGCTCTGAA AGACCAGCTA 
CTGGCAGCCA AACATATCCA ATCAAAGGCC ATTGAGGTTC GTCACGCTTC CTTGATGAAA 
CGCTGGAATC AGCTGCTTGC TAATTCTGCA GCCAGGAAAA AGAAACTCTT GGAGGCTCAG 
GAGCACTTCA GAAAGGTTGA AGATCTCTTC TTGACTTTTG CCAAGAAGGC CTCTGCCTTC 
AACAGTTGGT TTGAGAATGC TGAGGAGGAC CTGACGGATC CCGTGCGCTG CAATTCCCTG 
GAAGAAATCA AAGCACTGCG AGAAGCCCAC GATGCGTTCC GTTCCTCACT TAGCTCTGCC 
CAAGCTGACT TCAACCAGCT GGCAGAGCTT GATCGGCAGA TCAAGAGCTT CCGTGTAGCC 
TCCAACCCGT ACACTTGGTT TACTATGGAG GCTCTTGAAG AAACTTGGAG GAATCTGCAG 
AAAATTATCA AGGAGCGTGA ATTGGAGTTG CAGAAGGAGC AGCGGAGGCA GGAAGAAAAT 
GACAAGTTGC GCCAGGAGTT TGCTCAGCAT GCTAATGCCT TCCACCAGTG GATTCAGGAG 
ACAAGGACTT ACCTGCTAGA TGGGTCCTGC ATGGTGGAGG AGTCGGGAAC ACTGGAATCC 
CAGCTGGAAG CTACTAAACG CAAGCACCAG GAGATCCGGG CTATGAGGAG CCAGCTGAAG 
AAGATTGAGG ACCTTGGAGC AGCCATGGAA GAGGCACTTA TCTTGGACAA CAAATACACA 
GAGCACAGCA CCGTGGGGCT GGCGCAGCAG TGGGACCAGC TTGACCAGCT GGGAATGAGA 
ATGCAGCACA ATCTGGAACA GCAGATTCAA GCTCGAAACA CAACTGGAGT CACAGAGGAG 
GCCCTGAAGG AGTTCAGCAT GATGTTCAAG CACTTTGACA AGGACAAATC TGGACGTCTT 
AATCACCAGG AGTTTAAGTC TTGCTTGCGC TCCCTTGGCT ATGACCTGCC CATGGTTGAG 
GAAGGAGAGC CTGACCCCGA ATTTGAGTCT ATTCTTGACA CTGTTGATCC CAACAGGGAT 
GGCCACGTCT CGCTGCAGGA GTACATGGCG TTCATGATCA GCAGGGAAAC AGAGAACGTG 
AAATCCAGCG AGGAGATTGA GAGTGCTTTC CGTGCCCTCA GCTCGGAGGG GAAGCCTTAC 
GTGACCAAGG AGGAGCTCTA CCAGAACCTG ACCCGGGAGC AGGCCGACTA CTGCATTTCT 
CACATGAAGC CCTACATGGA TGGCAAGGGC AGAGAACTTC CTTCTGCCTA CGACTACATA 
GAATTTACAC GTTCACTCTT TGTGAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015134777.1
CDS	10..7479
Translation	MLSSFRKVKVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSSLRRQKLEDSYRFQFFQRDADELEKWIQEKLQIASDENYKDPSNLQGKLQKHQAFEAEVQANSGAIVKLDETGNQMINEGHFASETIRTRLQELHRLWELLLEKMREKGVKLLQAQKLVQFLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDLAAHEERVNEVNQFAGKLIQEQHPEEELIKSKQDEVNASWQRLKGLAQQRQGKLFGAAEVQRFNRDVDETISWIKEKGQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPINASQIQVKREELIANWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFRSADESGQALLAAGHYASDEVKEKLTILSDERSALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMDDVATRRDALLSRRNALHERAMKRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYASDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTSVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDADNIKKKQEALVARYEALKDPMVARKQKLADSLRLQQLFRDIEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKIKLNELNQKWDSLKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPTDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTLITKEVGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTNEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVANDLESEGLMAEEVQAVEHQEVYGMMPRDETDSKTASPWKSARMMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAQRLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKEKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLARGHYASPEIKEKLDILDQERTDLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAVLQSFADQLIAADHYAKGVIANRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIEMGNSLIERGACAGSEDAVKARLAALADQWEFLVQKSSEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRETINGRFQRIKSMAAARRAKLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVDHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCANGEDLIKKNNHHVENITAKMKGLKGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHIQSKAIEVRHASLMKRWNQLLANSAARKKKLLEAQEHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFESILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCISHMKPYMDGKGRELPSAYDYIEFTRSLFVN

Target ORF information:

RefSeq Version	XM_015279291.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279291.1

ORF Insert Sequence:

ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG 
TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC 
TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT 
GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT 
GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG 
ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAA CTCGTCTACA GGAGCTGCAC 
CGACTATGGG AGTTACTGCT GGAAAAAATG AGAGAGAAGG GAGTGAAGCT GTTGCAAGCA 
CAGAAGCTGG TGCAATTCTT ACGGGAATGT GAAGATGTCA TGGACTGGAT CAATGACAAG 
GAAGCAATAG TGACCTCAGA AGAGCTTGGA CAGGACTTAG AGCATGTTGA GGTTTTGCAG 
AAGAAATTTG AAGAGTTCCA AACAGATCTG GCAGCTCATG AGGAGAGAGT GAATGAAGTG 
AACCAGTTCG CTGGCAAACT TATCCAGGAA CAGCACCCTG AGGAGGAACT TATAAAGTCC 
AAACAGGATG AAGTAAATGC AAGCTGGCAG CGTCTTAAGG GGCTTGCCCA ACAGAGGCAA 
GGAAAACTCT TTGGGGCAGC TGAAGTTCAG CGCTTCAACA GGGATGTGGA TGAAACTATC 
AGCTGGATTA AGGAGAAAGG GCAGTTGATG GCCTCAGATG ACTTTGGCAG AGACTTGGCC 
AGCGTGCAAG CTTTACTACG TAAGCATGAA GGCCTGGAAA GAGATCTTGC AGCTTTGGAG 
GATAAGGTTA AGGCCCTCTG TGCAGAAGCT GACCGTTTGC AGCAGTCTCA CCCTATAAAT 
GCTTCTCAAA TTCAAGTGAA ACGGGAGGAA CTGATTGCCA ACTGGGAGCA GATCCGAACT 
CTGGCAGCAG AGAGGCATGC TCGCCTTAAT GACTCCTACA GGTTGCAGCG CTTTCTTGCA 
GATTTCCGGG ACCTCACTAG CTGGGTAACT GAGATGAAGG CTCTGATAAA TGCTGATGAG 
CTTGCCAATG ATGTGGCTGG AGCAGAAGCC CTTCTAGATA GACATCAGGA ACATAAGGGA 
GAAATTGATG CTCATGAGGA TAGCTTCAGA TCTGCTGATG AGTCTGGGCA GGCTTTGCTT 
GCAGCTGGGC ACTATGCTTC TGATGAAGTT AAAGAAAAGC TGACTATCCT CTCAGATGAA 
AGATCAGCCT TGCTGGAACT ATGGGAGCTT CGCAGGCAAC AGTATGAGCA GTGCATGGAT 
CTGCAGCTTT TCTACAGAGA TACTGAACAA GTTGACAACT GGATGAGCAA ACAAGAAGCT 
TTTCTGCTGA ACGAAGACCT TGGTGATTCT CTGGATAGCG TGGAGGCTCT TCTAAAGAAG 
CATGAAGATT TTGAGAAATC CCTAAGTGCT CAAGAGGAGA AGATCACAGC ACTGGATGAG 
TTTGCTACTA AACTGATTCA AAATAACCAT TATGCCATGG ATGACGTTGC TACGCGCAGA 
GATGCTCTTC TGAGTCGCCG AAATGCTCTT CATGAAAGAG CCATGAAGCG CCGTGCCCAA 
CTGGCAGACT CTTTCCATCT CCAGCAGTTT TTCAGGGACT CTGATGAACT GAAGAGTTGG 
GTTAATGAAA AAATGAAAAC TGCTACCGAT GAGGCTTACA AGGATCCATC CAACTTGCAA 
GGTAAAGTTC AGAAGCATCA GGCTTTTGAA GCAGAGCTTT CTGCTAATCA GAGTCGTATT 
GATGCACTGG AGAAAGCTGG CCAGAAGTTG ATTGATGTCA ATCACTATGC ATCTGATGAG 
GTGGCAGCTC GCATGAATGA AGTCATCAGC TTGTGGAAGA AACTTCTGGA AGCCACCGAG 
CTCAAAGGTA TAAAGCTGCG TGAAGCCAAT CAACAGCAGC AATTTAATCG CAATGTAGAA 
GACATTGAGT TGTGGCTGTA TGAAGTGGAG GGTCACTTGG CTTCTGATGA TTATGGAAAA 
GATCTTACCA GCGTTCAGAA TCTTCAGAAG AAACATGCCC TGCTAGAGGC AGATGTTGCT 
GCCCATCAGG ATCGGATAGA TGGCATTACC ATCCAGGCAC GCCAGTTCCA AGATGCTGGG 
CACTTTGATG CTGACAATAT CAAGAAGAAA CAAGAAGCTT TAGTAGCTCG TTATGAAGCT 
CTGAAGGATC CTATGGTGGC TCGCAAGCAG AAACTTGCAG ATTCTCTTCG CCTGCAGCAG 
CTTTTCCGTG ACATCGAGGA TGAAGAGACC TGGATCAGGG AAAAGGAACC TATTGCAGCC 
TCAACAAACC GAGGCAAGGA CTTAATTGGT GTCCAGAATC TGCTAAAGAA GCACCAGGCT 
TTGCAGGCAG AAATTGCAGG CCATGAGCCT CGCATTAAAG CAGTCACACA AAAAGGAAAT 
GCGATGGTGG AAGAGGGACA TTTTGCTGCT GAAGATGTGA AGATCAAATT GAATGAACTA 
AACCAAAAAT GGGACTCTCT GAAAGCCAAG GCATCTCAAC GGCGACAGGA TCTAGAGGAT 
TCTCTGCAAG CTCAGCAGTA TTTTGCTGAT GCTAATGAGG CAGAATCATG GATGAGGGAA 
AAGGAGCCCA TTGTAGGCAG CACGGATTAT GGAAAGGATG AAGACTCTGC TGAGGCTCTC 
CTGAAGAAGC ATGAAGCTTT GATGTCTGAT CTTTCTGCTT ACGGCAGTAG CATACAGGCA 
TTAAGGGAAC AGGCCCAGTC CTGCAGGCAA CAAGTTGCTC CCACTGATGA TGAAACTGGA 
AAAGAGCTTG TGCTAGCACT CTATGATTAC CAAGAGAAGA GTCCTCGGGA GGTGACTATG 
AAGAAAGGAG ATATTCTAAC CCTGCTCAAC AGCACCAACA AGGACTGGTG GAAGGTTGAA 
GTTAACGATC GTCAGGGCTT TGTACCAGCT GCCTATGTGA AAAAACTAGA TCCTGCCCAG 
TCTGCATCCC GAGAGAATCT TCTAGAAGAG CAAGGCAGCA TAGCATTGCG ACAGGAGCAA 
ATTGACAACC AGACTCTCAT TACTAAGGAA GTCGGCAGTG TATCTCTGCG TATGAAACAG 
GTCGAAGAAC TGTATCATTC CCTTCTTGAG CTGGGAGAAA AACGTAAAGG CATGCTAGAA 
AAAAGCTGCA AGAAGTTTAT GCTTTTCCGT GAGGCTAATG AGCTTCAACA GTGGATCAAT 
GAGAAGGAAG CTGCGCTCAC TAATGAGGAA GTGGGTGCTG ATTTGGAGCA GGTGGAGGTG 
CTACAGAAGA AGTTTGATGA TTTTCAGAAG GATTTAAAAG CTAATGAGTC ACGACTGAAG 
GACATAAACA AGGTTGCAAA TGACCTGGAG TCTGAAGGGC TGATGGCAGA GGAAGTGCAA 
GCAGTAGAGC ACCAGGAAGT CTATGGAATG ATGCCCAGAG ATGAAACTGA TTCTAAGACA 
GCCTCTCCTT GGAAGTCTGC ACGTATGATG GTACACACAG TGGCAACGTT TAACTCAATC 
AAGGAACTGA ATGAACGCTG GAGATCCCTG CAGCAGTTGG CAGAGGAGCG AAGCCAGCTG 
TTGGGCAGCG CTCATGAAGT CCAGAGGTTC CACAGAGATG CTGATGAAAC AAAAGAATGG 
ATAGAAGAGA AGAATCAAGC ATTAAATACC GACAACTATG GACATGACTT GGCCAGTGTG 
CAGGCTCTGC AGCGCAAGCA TGAAGGCTTT GAGAGAGACT TGGCAGCTCT TGGAGACAAG 
GTGAATTCTC TTGGTGAAAC TGCCCAGCGT CTGATCCAGT CACATCCAGA ATCAGCTGAA 
GATCTTCAAG AAAAATGTAC TGAGTTGAAC CAAGCCTGGA GTAGTCTGGG AAAACGTGCT 
GACCAACGCA AAGAGAAGCT TGGAGATTCT CATGACCTGC AGCGTTTCCT CAGTGATTTT 
AGAGACCTTA TGTCTTGGAT CAATGGAATC CGGGGTCTGG TCTCCTCAGA TGAACTTGCA 
AAAGATGTGA CTGGAGCTGA AGCTCTGTTG GAAAGGCATC AGGAGCACCG TACTGAAATA 
GATGCACGGG CTGGCACTTT TCAGGCATTT GAACAGTTTG GACAGCAGCT TCTGGCTCGT 
GGACACTATG CCAGCCCAGA GATTAAGGAG AAACTGGATA TTCTAGATCA AGAACGGACA 
GACCTAGAGA AGGCCTGGGT CCAGCGCAGA ATGATGCTAG ACCAGTGCTT AGAACTACAG 
CTGTTTCATC GGGATTGTGA ACAAGCTGAA AACTGGATGG CTGCCCGAGA GGCGTTCCTA 
AATACAGAAG ACAAAGGAGA CTCCTTAGAC AGCGTGGAGG CACTCATCAA GAAGCATGAA 
GATTTCGATA AAGCAATCAA TGTCCAGGAA GAGAAAATTG CTGTCTTGCA GTCTTTTGCT 
GACCAACTGA TTGCTGCTGA TCATTATGCA AAAGGAGTCA TTGCTAACAG ACGCAATGAG 
GTTCTGGACA GGTGGCGTCG TCTGAAGGCT CAGATGATTG AGAAGAGATC TAAGCTGGGA 
GAATCTCAGA CCCTCCAACA GTTCAGTCGT GATGTTGATG AAATAGAAGC TTGGATCAGT 
GAAAAGCTTC AAACTGCAAG TGATGAGTCA TATAAGGATC CCACAAACAT CCAGCTTTCC 
AAACTGCTGA GCAAGCACCA GAAGCACCAA GCCTTTGAAG CTGAGCTCCA CGCCAACGCA 
GATCGGATTC GTGGAGTCAT TGAAATGGGG AACTCTCTTA TTGAAAGAGG AGCGTGTGCT 
GGCAGCGAGG ATGCTGTGAA GGCTCGGCTG GCTGCCCTGG CTGACCAATG GGAGTTCCTG 
GTCCAGAAGT CATCAGAGAA GAGTCAGAAA CTGAAAGAAG CAAATAAACA GCAGAATTTC 
AATACCGGCA TCAAGGACTT TGATTTCTGG CTTTCAGAGG TGGAAGCTTT GTTGGCATCT 
GAAGACTATG GGAAGGACTT GGCATCAGTG AACAACCTTC TGAAGAAGCA CCAATTACTG 
GAAGCTGATA TATCTGCTCA TGAGGATCGG CTGAAGGACC TGAACAGCCA GGCTGACAGT 
TTGATGACCA GCAGTGCTTT CGATACCTCC CAAGTAAAGG ATAAACGTGA AACTATAAAT 
GGGCGCTTCC AGAGAATCAA GAGCATGGCA GCTGCCCGCC GTGCGAAGCT CAACGAGTCG 
CACCGCTTGC ATCAGTTCTT CCGTGACATG GATGATGAGG AGTCCTGGAT CAAAGAGAAG 
AAACTGTTGG TGAGCTCAGA GGACTATGGC AGAGACCTGA CTGGTGTGCA GAACCTGAGG 
AAGAAACATA AGCGCTTGGA AGCAGAATTA GCTGCCCATG AACCTGCTAT CCAGGGTGTT 
CTAGACACTG GCAAGAAGCT TTCAGATGAT AACACAATTG GGAAGGAGGA GATACAGCAA 
AGACTGGCTC AGTTTGTGGA CCACTGGAAA GAGTTAAAAC AGCTGGCAGC TGCTCGGGGG 
CAGCGTCTGG AGGAGTCCCT GGAGTACCAA CAGTTTGTAG CAAATGTTGA GGAAGAGGAG 
GCATGGATCA ACGAGAAAAT GACATTGGTA GCCAGTGAGG ATTATGGAGA CACACTTGCT 
GCTATCCAGG GCTTGCTGAA GAAGCATGAA GCATTCGAGA CTGACTTTAC TGTCCACAAA 
GACAGAGTGA ATGATGTTTG CGCTAATGGA GAGGATCTCA TTAAAAAGAA TAATCACCAT 
GTGGAGAACA TTACTGCTAA GATGAAGGGC CTCAAGGGCA AGGTATCAGA TCTGGAGAAA 
GCCGCAGCTC AGAGGAAAGC CAAACTGGAT GAGAACTCTG CCTTCCTCCA GTTCAACTGG 
AAAGCAGATG TGGTGGAGTC ATGGATAGGT GAGAAGGAAA ACAGTCTGAA GACAGATGAT 
TATGGCCGTG ACCTCTCCTC TGTGCAAACA CTACTCACCA AACAGGAAAC CTTTGATGCT 
GGACTTCAGG CTTTCCAGCA GGAGGGAATT GCAAACATCA CTGCTCTGAA AGACCAGCTA 
CTGGCAGCCA AACATATCCA ATCAAAGGCC ATTGAGGTTC GTCACGCTTC CTTGATGAAA 
CGCTGGAATC AGCTGCTTGC TAATTCTGCA GCCAGGAAAA AGAAACTCTT GGAGGCTCAG 
GAGCACTTCA GAAAGGTTGA AGATCTCTTC TTGACTTTTG CCAAGAAGGC CTCTGCCTTC 
AACAGTTGGT TTGAGAATGC TGAGGAGGAC CTGACGGATC CCGTGCGCTG CAATTCCCTG 
GAAGAAATCA AAGCACTGCG AGAAGCCCAC GATGCGTTCC GTTCCTCACT TAGCTCTGCC 
CAAGCTGACT TCAACCAGCT GGCAGAGCTT GATCGGCAGA TCAAGAGCTT CCGTGTAGCC 
TCCAACCCGT ACACTTGGTT TACTATGGAG GCTCTTGAAG AAACTTGGAG GAATCTGCAG 
AAAATTATCA AGGAGCGTGA ATTGGAGTTG CAGAAGGAGC AGCGGAGGCA GGAAGAAAAT 
GACAAGTTGC GCCAGGAGTT TGCTCAGCAT GCTAATGCCT TCCACCAGTG GATTCAGGAG 
ACAAGGACTT ACCTGCTAGA TGGGTCCTGC ATGGTGGAGG AGTCGGGAAC ACTGGAATCC 
CAGCTGGAAG CTACTAAACG CAAGCACCAG GAGATCCGGG CTATGAGGAG CCAGCTGAAG 
AAGATTGAGG ACCTTGGAGC AGCCATGGAA GAGGCACTTA TCTTGGACAA CAAATACACA 
GAGCACAGCA CCGTGGGGCT GGCGCAGCAG TGGGACCAGC TTGACCAGCT GGGAATGAGA 
ATGCAGCACA ATCTGGAACA GCAGATTCAA GCTCGAAACA CAACTGGAGT CACAGAGGAG 
GCCCTGAAGG AGTTCAGCAT GATGTTCAAG CACTTTGACA AGGACAAATC TGGACGTCTT 
AATCACCAGG AGTTTAAGTC TTGCTTGCGC TCCCTTGGCT ATGACCTGCC CATGGTTGAG 
GAAGGAGAGC CTGACCCCGA ATTTGAGTCT ATTCTTGACA CTGTTGATCC CAACAGGGAT 
GGCCACGTCT CGCTGCAGGA GTACATGGCG TTCATGATCA GCAGGGAAAC AGAGAACGTG 
AAATCCAGCG AGGAGATTGA GAGTGCTTTC CGTGCCCTCA GCTCGGAGGG GAAGCCTTAC 
GTGACCAAGG AGGAGCTCTA CCAGAACCTG ACCCGGGAGC AGGCCGACTA CTGCATTTCT 
CACATGAAGC CCTACATGGA TGGCAAGGGC AGAGAACTTC CTTCTGCCTA CGACTACATA 
GAATTTACAC GTTCACTCTT TGTGAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_015279292.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015134778.1 Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X11, mRNA.

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CloneID	OGa43318
Clone ID Related Accession (Same CDS sequence)	XM_015279292.2 , XM_015279292.1
Accession Version	XM_015279292.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7455bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015279292.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG 
TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC 
TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT 
GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT 
GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG 
ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAA CTCGTCTACA GGAGCTGCAC 
CGACTATGGG AGTTACTGCT GGAAAAAATG AGAGAGAAGG GAGTGAAGCT GTTGCAAGCA 
CAGAAGCTGG TGCAATTCTT ACGGGAATGT GAAGATGTCA TGGACTGGAT CAATGACAAG 
GAAGCAATAG TGACCTCAGA AGAGCTTGGA CAGGACTTAG AGCATGTTGA GGTTTTGCAG 
AAGAAATTTG AAGAGTTCCA AACAGATCTG GCAGCTCATG AGGAGAGAGT GAATGAAGTG 
AACCAGTTCG CTGGCAAACT TATCCAGGAA CAGCACCCTG AGGAGGAACT TATAAAGTCC 
AAACAGGATG AAGTAAATGC AAGCTGGCAG CGTCTTAAGG GGCTTGCCCA ACAGAGGCAA 
GGAAAACTCT TTGGGGCAGC TGAAGTTCAG CGCTTCAACA GGGATGTGGA TGAAACTATC 
AGCTGGATTA AGGAGAAAGG GCAGTTGATG GCCTCAGATG ACTTTGGCAG AGACTTGGCC 
AGCGTGCAAG CTTTACTACG TAAGCATGAA GGCCTGGAAA GAGATCTTGC AGCTTTGGAG 
GATAAGGTTA AGGCCCTCTG TGCAGAAGCT GACCGTTTGC AGCAGTCTCA CCCTATAAAT 
GCTTCTCAAA TTCAAGTGAA ACGGGAGGAA CTGATTGCCA ACTGGGAGCA GATCCGAACT 
CTGGCAGCAG AGAGGCATGC TCGCCTTAAT GACTCCTACA GGTTGCAGCG CTTTCTTGCA 
GATTTCCGGG ACCTCACTAG CTGGGTAACT GAGATGAAGG CTCTGATAAA TGCTGATGAG 
CTTGCCAATG ATGTGGCTGG AGCAGAAGCC CTTCTAGATA GACATCAGGA ACATAAGGGA 
GAAATTGATG CTCATGAGGA TAGCTTCAGA TCTGCTGATG AGTCTGGGCA GGCTTTGCTT 
GCAGCTGGGC ACTATGCTTC TGATGAAGTT AAAGAAAAGC TGACTATCCT CTCAGATGAA 
AGATCAGCCT TGCTGGAACT ATGGGAGCTT CGCAGGCAAC AGTATGAGCA GTGCATGGAT 
CTGCAGCTTT TCTACAGAGA TACTGAACAA GTTGACAACT GGATGAGCAA ACAAGAAGCT 
TTTCTGCTGA ACGAAGACCT TGGTGATTCT CTGGATAGCG TGGAGGCTCT TCTAAAGAAG 
CATGAAGATT TTGAGAAATC CCTAAGTGCT CAAGAGGAGA AGATCACAGC ACTGGATGAG 
TTTGCTACTA AACTGATTCA AAATAACCAT TATGCCATGG ATGACGTTGC TACGCGCAGA 
GATGCTCTTC TGAGTCGCCG AAATGCTCTT CATGAAAGAG CCATGAAGCG CCGTGCCCAA 
CTGGCAGACT CTTTCCATCT CCAGCAGTTT TTCAGGGACT CTGATGAACT GAAGAGTTGG 
GTTAATGAAA AAATGAAAAC TGCTACCGAT GAGGCTTACA AGGATCCATC CAACTTGCAA 
GGTAAAGTTC AGAAGCATCA GGCTTTTGAA GCAGAGCTTT CTGCTAATCA GAGTCGTATT 
GATGCACTGG AGAAAGCTGG CCAGAAGTTG ATTGATGTCA ATCACTATGC ATCTGATGAG 
GTGGCAGCTC GCATGAATGA AGTCATCAGC TTGTGGAAGA AACTTCTGGA AGCCACCGAG 
CTCAAAGGTA TAAAGCTGCG TGAAGCCAAT CAACAGCAGC AATTTAATCG CAATGTAGAA 
GACATTGAGT TGTGGCTGTA TGAAGTGGAG GGTCACTTGG CTTCTGATGA TTATGGAAAA 
GATCTTACCA GCGTTCAGAA TCTTCAGAAG AAACATGCCC TGCTAGAGGC AGATGTTGCT 
GCCCATCAGG ATCGGATAGA TGGCATTACC ATCCAGGCAC GCCAGTTCCA AGATGCTGGG 
CACTTTGATG CTGACAATAT CAAGAAGAAA CAAGAAGCTT TAGTAGCTCG TTATGAAGCT 
CTGAAGGATC CTATGGTGGC TCGCAAGCAG AAACTTGCAG ATTCTCTTCG CCTGCAGCAG 
CTTTTCCGTG ACATCGAGGA TGAAGAGACC TGGATCAGGG AAAAGGAACC TATTGCAGCC 
TCAACAAACC GAGGCAAGGA CTTAATTGGT GTCCAGAATC TGCTAAAGAA GCACCAGGCT 
TTGCAGGCAG AAATTGCAGG CCATGAGCCT CGCATTAAAG CAGTCACACA AAAAGGAAAT 
GCGATGGTGG AAGAGGGACA TTTTGCTGCT GAAGATGTGA AGATCAAATT GAATGAACTA 
AACCAAAAAT GGGACTCTCT GAAAGCCAAG GCATCTCAAC GGCGACAGGA TCTAGAGGAT 
TCTCTGCAAG CTCAGCAGTA TTTTGCTGAT GCTAATGAGG CAGAATCATG GATGAGGGAA 
AAGGAGCCCA TTGTAGGCAG CACGGATTAT GGAAAGGATG AAGACTCTGC TGAGGCTCTC 
CTGAAGAAGC ATGAAGCTTT GATGTCTGAT CTTTCTGCTT ACGGCAGTAG CATACAGGCA 
TTAAGGGAAC AGGCCCAGTC CTGCAGGCAA CAAGTTGCTC CCACTGATGA TGAAACTGGA 
AAAGAGCTTG TGCTAGCACT CTATGATTAC CAAGAGAAGA GTCCTCGGGA GGTGACTATG 
AAGAAAGGAG ATATTCTAAC CCTGCTCAAC AGCACCAACA AGGACTGGTG GAAGGTTGAA 
GTTAACGATC GTCAGGGCTT TGTACCAGCT GCCTATGTGA AAAAACTAGA TCCTGCCCAG 
TCTGCATCCC GAGAGAATCT TCTAGAAGAG CAAGGCAGCA TAGCATTGCG ACAGGAGCAA 
ATTGACAACC AGACTCTCAT TACTAAGGAA GTCGGCAGTG TATCTCTGCG TATGAAACAG 
GTCGAAGAAC TGTATCATTC CCTTCTTGAG CTGGGAGAAA AACGTAAAGG CATGCTAGAA 
AAAAGCTGCA AGAAGTTTAT GCTTTTCCGT GAGGCTAATG AGCTTCAACA GTGGATCAAT 
GAGAAGGAAG CTGCGCTCAC TAATGAGGAA GTGGGTGCTG ATTTGGAGCA GGTGGAGGTG 
CTACAGAAGA AGTTTGATGA TTTTCAGAAG GATTTAAAAG CTAATGAGTC ACGACTGAAG 
GACATAAACA AGGTTGCAAA TGACCTGGAG TCTGAAGGGC TGATGGCAGA GGAAGTGCAA 
GCAGTAGAGC ACCAGGAAGT CTATGGAATG ATGCCCAGAG ATGAAACTGA TTCTAAGACA 
GCCTCTCCTT GGAAGTCTGC ACGTATGATG GTACACACAG TGGCAACGTT TAACTCAATC 
AAGGAACTGA ATGAACGCTG GAGATCCCTG CAGCAGTTGG CAGAGGAGCG AAGCCAGCTG 
TTGGGCAGCG CTCATGAAGT CCAGAGGTTC CACAGAGATG CTGATGAAAC AAAAGAATGG 
ATAGAAGAGA AGAATCAAGC ATTAAATACC GACAACTATG GACATGACTT GGCCAGTGTG 
CAGGCTCTGC AGCGCAAGCA TGAAGGCTTT GAGAGAGACT TGGCAGCTCT TGGAGACAAG 
GTGAATTCTC TTGGTGAAAC TGCCCAGCGT CTGATCCAGT CACATCCAGA ATCAGCTGAA 
GATCTTCAAG AAAAATGTAC TGAGTTGAAC CAAGCCTGGA GTAGTCTGGG AAAACGTGCT 
GACCAACGCA AAGAGAAGCT TGGAGATTCT CATGACCTGC AGCGTTTCCT CAGTGATTTT 
AGAGACCTTA TGTCTTGGAT CAATGGAATC CGGGGTCTGG TCTCCTCAGA TGAACTTGCA 
AAAGATGTGA CTGGAGCTGA AGCTCTGTTG GAAAGGCATC AGGAGCACCG TACTGAAATA 
GATGCACGGG CTGGCACTTT TCAGGCATTT GAACAGTTTG GACAGCAGCT TCTGGCTCGT 
GGACACTATG CCAGCCCAGA GATTAAGGAG AAACTGGATA TTCTAGATCA AGAACGGACA 
GACCTAGAGA AGGCCTGGGT CCAGCGCAGA ATGATGCTAG ACCAGTGCTT AGAACTACAG 
CTGTTTCATC GGGATTGTGA ACAAGCTGAA AACTGGATGG CTGCCCGAGA GGCGTTCCTA 
AATACAGAAG ACAAAGGAGA CTCCTTAGAC AGCGTGGAGG CACTCATCAA GAAGCATGAA 
GATTTCGATA AAGCAATCAA TGTCCAGGAA GAGAAAATTG CTGTCTTGCA GTCTTTTGCT 
GACCAACTGA TTGCTGCTGA TCATTATGCA AAAGGAGTCA TTGCTAACAG ACGCAATGAG 
GTTCTGGACA GGTGGCGTCG TCTGAAGGCT CAGATGATTG AGAAGAGATC TAAGCTGGGA 
GAATCTCAGA CCCTCCAACA GTTCAGTCGT GATGTTGATG AAATAGAAGC TTGGATCAGT 
GAAAAGCTTC AAACTGCAAG TGATGAGTCA TATAAGGATC CCACAAACAT CCAGAGCAAG 
CACCAGAAGC ACCAAGCCTT TGAAGCTGAG CTCCACGCCA ACGCAGATCG GATTCGTGGA 
GTCATTGAAA TGGGGAACTC TCTTATTGAA AGAGGAGCGT GTGCTGGCAG CGAGGATGCT 
GTGAAGGCTC GGCTGGCTGC CCTGGCTGAC CAATGGGAGT TCCTGGTCCA GAAGTCATCA 
GAGAAGAGTC AGAAACTGAA AGAAGCAAAT AAACAGCAGA ATTTCAATAC CGGCATCAAG 
GACTTTGATT TCTGGCTTTC AGAGGTGGAA GCTTTGTTGG CATCTGAAGA CTATGGGAAG 
GACTTGGCAT CAGTGAACAA CCTTCTGAAG AAGCACCAAT TACTGGAAGC TGATATATCT 
GCTCATGAGG ATCGGCTGAA GGACCTGAAC AGCCAGGCTG ACAGTTTGAT GACCAGCAGT 
GCTTTCGATA CCTCCCAAGT AAAGGATAAA CGTGAAACTA TAAATGGGCG CTTCCAGAGA 
ATCAAGAGCA TGGCAGCTGC CCGCCGTGCG AAGCTCAACG AGTCGCACCG CTTGCATCAG 
TTCTTCCGTG ACATGGATGA TGAGGAGTCC TGGATCAAAG AGAAGAAACT GTTGGTGAGC 
TCAGAGGACT ATGGCAGAGA CCTGACTGGT GTGCAGAACC TGAGGAAGAA ACATAAGCGC 
TTGGAAGCAG AATTAGCTGC CCATGAACCT GCTATCCAGG GTGTTCTAGA CACTGGCAAG 
AAGCTTTCAG ATGATAACAC AATTGGGAAG GAGGAGATAC AGCAAAGACT GGCTCAGTTT 
GTGGACCACT GGAAAGAGTT AAAACAGCTG GCAGCTGCTC GGGGGCAGCG TCTGGAGGAG 
TCCCTGGAGT ACCAACAGTT TGTAGCAAAT GTTGAGGAAG AGGAGGCATG GATCAACGAG 
AAAATGACAT TGGTAGCCAG TGAGGATTAT GGAGACACAC TTGCTGCTAT CCAGGGCTTG 
CTGAAGAAGC ATGAAGCATT CGAGACTGAC TTTACTGTCC ACAAAGACAG AGTGAATGAT 
GTTTGCGCTA ATGGAGAGGA TCTCATTAAA AAGAATAATC ACCATGTGGA GAACATTACT 
GCTAAGATGA AGGGCCTCAA GGGCAAGGTA TCAGATCTGG AGAAAGCCGC AGCTCAGAGG 
AAAGCCAAAC TGGATGAGAA CTCTGCCTTC CTCCAGTTCA ACTGGAAAGC AGATGTGGTG 
GAGTCATGGA TAGGTGAGAA GGAAAACAGT CTGAAGACAG ATGATTATGG CCGTGACCTC 
TCCTCTGTGC AAACACTACT CACCAAACAG GAAACCTTTG ATGCTGGACT TCAGGCTTTC 
CAGCAGGAGG GAATTGCAAA CATCACTGCT CTGAAAGACC AGCTACTGGC AGCCAAACAT 
ATCCAATCAA AGGCCATTGA GGTTCGTCAC GCTTCCTTGA TGAAACGCTG GAATCAGCTG 
CTTGCTAATT CTGCAGCCAG GAAAAAGAAA CTCTTGGAGG CTCAGGAGCA CTTCAGAAAG 
GTTGAAGATC TCTTCTTGAC TTTTGCCAAG AAGGCCTCTG CCTTCAACAG TTGGTTTGAG 
AATGCTGAGG AGGACCTGAC GGATCCCGTG CGCTGCAATT CCCTGGAAGA AATCAAAGCA 
CTGCGAGAAG CCCACGATGC GTTCCGTTCC TCACTTAGCT CTGCCCAAGC TGACTTCAAC 
CAGCTGGCAG AGCTTGATCG GCAGATCAAG AGCTTCCGTG TAGCCTCCAA CCCGTACACT 
TGGTTTACTA TGGAGGCTCT TGAAGAAACT TGGAGGAATC TGCAGAAAAT TATCAAGGAG 
CGTGAATTGG AGTTGCAGAA GGAGCAGCGG AGGCAGGAAG AAAATGACAA GTTGCGCCAG 
GAGTTTGCTC AGCATGCTAA TGCCTTCCAC CAGTGGATTC AGGAGACAAG GACTTACCTG 
CTAGATGGGT CCTGCATGGT GGAGGAGTCG GGAACACTGG AATCCCAGCT GGAAGCTACT 
AAACGCAAGC ACCAGGAGAT CCGGGCTATG AGGAGCCAGC TGAAGAAGAT TGAGGACCTT 
GGAGCAGCCA TGGAAGAGGC ACTTATCTTG GACAACAAAT ACACAGAGCA CAGCACCGTG 
GGGCTGGCGC AGCAGTGGGA CCAGCTTGAC CAGCTGGGAA TGAGAATGCA GCACAATCTG 
GAACAGCAGA TTCAAGCTCG AAACACAACT GGAGTCACAG AGGAGGCCCT GAAGGAGTTC 
AGCATGATGT TCAAGCACTT TGACAAGGAC AAATCTGGAC GTCTTAATCA CCAGGAGTTT 
AAGTCTTGCT TGCGCTCCCT TGGCTATGAC CTGCCCATGG TTGAGGAAGG AGAGCCTGAC 
CCCGAATTTG AGTCTATTCT TGACACTGTT GATCCCAACA GGGATGGCCA CGTCTCGCTG 
CAGGAGTACA TGGCGTTCAT GATCAGCAGG GAAACAGAGA ACGTGAAATC CAGCGAGGAG 
ATTGAGAGTG CTTTCCGTGC CCTCAGCTCG GAGGGGAAGC CTTACGTGAC CAAGGAGGAG 
CTCTACCAGA ACCTGACCCG GGAGCAGGCC GACTACTGCA TTTCTCACAT GAAGCCCTAC 
ATGGATGGCA AGGGCAGAGA ACTTCCTTCT GCCTACGACT ACATAGAATT TACACGTTCA 
CTCTTTGTGA ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015134778.1
CDS	8..7462
Translation	MLSSFRKVKVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSSLRRQKLEDSYRFQFFQRDADELEKWIQEKLQIASDENYKDPSNLQGKLQKHQAFEAEVQANSGAIVKLDETGNQMINEGHFASETIRTRLQELHRLWELLLEKMREKGVKLLQAQKLVQFLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDLAAHEERVNEVNQFAGKLIQEQHPEEELIKSKQDEVNASWQRLKGLAQQRQGKLFGAAEVQRFNRDVDETISWIKEKGQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPINASQIQVKREELIANWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFRSADESGQALLAAGHYASDEVKEKLTILSDERSALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMDDVATRRDALLSRRNALHERAMKRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYASDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTSVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDADNIKKKQEALVARYEALKDPMVARKQKLADSLRLQQLFRDIEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKIKLNELNQKWDSLKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPTDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTLITKEVGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTNEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVANDLESEGLMAEEVQAVEHQEVYGMMPRDETDSKTASPWKSARMMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAQRLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKEKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLARGHYASPEIKEKLDILDQERTDLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAVLQSFADQLIAADHYAKGVIANRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQSKHQKHQAFEAELHANADRIRGVIEMGNSLIERGACAGSEDAVKARLAALADQWEFLVQKSSEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRETINGRFQRIKSMAAARRAKLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVDHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCANGEDLIKKNNHHVENITAKMKGLKGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHIQSKAIEVRHASLMKRWNQLLANSAARKKKLLEAQEHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFESILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCISHMKPYMDGKGRELPSAYDYIEFTRSLFVN

Target ORF information:

RefSeq Version	XM_015279292.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279292.2

ORF Insert Sequence:

ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG 
TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC 
TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT 
GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT 
GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG 
ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAA CTCGTCTACA GGAGCTGCAC 
CGACTATGGG AGTTACTGCT GGAAAAAATG AGAGAGAAGG GAGTGAAGCT GTTGCAAGCA 
CAGAAGCTGG TGCAATTCTT ACGGGAATGT GAAGATGTCA TGGACTGGAT CAATGACAAG 
GAAGCAATAG TGACCTCAGA AGAGCTTGGA CAGGACTTAG AGCATGTTGA GGTTTTGCAG 
AAGAAATTTG AAGAGTTCCA AACAGATCTG GCAGCTCATG AGGAGAGAGT GAATGAAGTG 
AACCAGTTCG CTGGCAAACT TATCCAGGAA CAGCACCCTG AGGAGGAACT TATAAAGTCC 
AAACAGGATG AAGTAAATGC AAGCTGGCAG CGTCTTAAGG GGCTTGCCCA ACAGAGGCAA 
GGAAAACTCT TTGGGGCAGC TGAAGTTCAG CGCTTCAACA GGGATGTGGA TGAAACTATC 
AGCTGGATTA AGGAGAAAGG GCAGTTGATG GCCTCAGATG ACTTTGGCAG AGACTTGGCC 
AGCGTGCAAG CTTTACTACG TAAGCATGAA GGCCTGGAAA GAGATCTTGC AGCTTTGGAG 
GATAAGGTTA AGGCCCTCTG TGCAGAAGCT GACCGTTTGC AGCAGTCTCA CCCTATAAAT 
GCTTCTCAAA TTCAAGTGAA ACGGGAGGAA CTGATTGCCA ACTGGGAGCA GATCCGAACT 
CTGGCAGCAG AGAGGCATGC TCGCCTTAAT GACTCCTACA GGTTGCAGCG CTTTCTTGCA 
GATTTCCGGG ACCTCACTAG CTGGGTAACT GAGATGAAGG CTCTGATAAA TGCTGATGAG 
CTTGCCAATG ATGTGGCTGG AGCAGAAGCC CTTCTAGATA GACATCAGGA ACATAAGGGA 
GAAATTGATG CTCATGAGGA TAGCTTCAGA TCTGCTGATG AGTCTGGGCA GGCTTTGCTT 
GCAGCTGGGC ACTATGCTTC TGATGAAGTT AAAGAAAAGC TGACTATCCT CTCAGATGAA 
AGATCAGCCT TGCTGGAACT ATGGGAGCTT CGCAGGCAAC AGTATGAGCA GTGCATGGAT 
CTGCAGCTTT TCTACAGAGA TACTGAACAA GTTGACAACT GGATGAGCAA ACAAGAAGCT 
TTTCTGCTGA ACGAAGACCT TGGTGATTCT CTGGATAGCG TGGAGGCTCT TCTAAAGAAG 
CATGAAGATT TTGAGAAATC CCTAAGTGCT CAAGAGGAGA AGATCACAGC ACTGGATGAG 
TTTGCTACTA AACTGATTCA AAATAACCAT TATGCCATGG ATGACGTTGC TACGCGCAGA 
GATGCTCTTC TGAGTCGCCG AAATGCTCTT CATGAAAGAG CCATGAAGCG CCGTGCCCAA 
CTGGCAGACT CTTTCCATCT CCAGCAGTTT TTCAGGGACT CTGATGAACT GAAGAGTTGG 
GTTAATGAAA AAATGAAAAC TGCTACCGAT GAGGCTTACA AGGATCCATC CAACTTGCAA 
GGTAAAGTTC AGAAGCATCA GGCTTTTGAA GCAGAGCTTT CTGCTAATCA GAGTCGTATT 
GATGCACTGG AGAAAGCTGG CCAGAAGTTG ATTGATGTCA ATCACTATGC ATCTGATGAG 
GTGGCAGCTC GCATGAATGA AGTCATCAGC TTGTGGAAGA AACTTCTGGA AGCCACCGAG 
CTCAAAGGTA TAAAGCTGCG TGAAGCCAAT CAACAGCAGC AATTTAATCG CAATGTAGAA 
GACATTGAGT TGTGGCTGTA TGAAGTGGAG GGTCACTTGG CTTCTGATGA TTATGGAAAA 
GATCTTACCA GCGTTCAGAA TCTTCAGAAG AAACATGCCC TGCTAGAGGC AGATGTTGCT 
GCCCATCAGG ATCGGATAGA TGGCATTACC ATCCAGGCAC GCCAGTTCCA AGATGCTGGG 
CACTTTGATG CTGACAATAT CAAGAAGAAA CAAGAAGCTT TAGTAGCTCG TTATGAAGCT 
CTGAAGGATC CTATGGTGGC TCGCAAGCAG AAACTTGCAG ATTCTCTTCG CCTGCAGCAG 
CTTTTCCGTG ACATCGAGGA TGAAGAGACC TGGATCAGGG AAAAGGAACC TATTGCAGCC 
TCAACAAACC GAGGCAAGGA CTTAATTGGT GTCCAGAATC TGCTAAAGAA GCACCAGGCT 
TTGCAGGCAG AAATTGCAGG CCATGAGCCT CGCATTAAAG CAGTCACACA AAAAGGAAAT 
GCGATGGTGG AAGAGGGACA TTTTGCTGCT GAAGATGTGA AGATCAAATT GAATGAACTA 
AACCAAAAAT GGGACTCTCT GAAAGCCAAG GCATCTCAAC GGCGACAGGA TCTAGAGGAT 
TCTCTGCAAG CTCAGCAGTA TTTTGCTGAT GCTAATGAGG CAGAATCATG GATGAGGGAA 
AAGGAGCCCA TTGTAGGCAG CACGGATTAT GGAAAGGATG AAGACTCTGC TGAGGCTCTC 
CTGAAGAAGC ATGAAGCTTT GATGTCTGAT CTTTCTGCTT ACGGCAGTAG CATACAGGCA 
TTAAGGGAAC AGGCCCAGTC CTGCAGGCAA CAAGTTGCTC CCACTGATGA TGAAACTGGA 
AAAGAGCTTG TGCTAGCACT CTATGATTAC CAAGAGAAGA GTCCTCGGGA GGTGACTATG 
AAGAAAGGAG ATATTCTAAC CCTGCTCAAC AGCACCAACA AGGACTGGTG GAAGGTTGAA 
GTTAACGATC GTCAGGGCTT TGTACCAGCT GCCTATGTGA AAAAACTAGA TCCTGCCCAG 
TCTGCATCCC GAGAGAATCT TCTAGAAGAG CAAGGCAGCA TAGCATTGCG ACAGGAGCAA 
ATTGACAACC AGACTCTCAT TACTAAGGAA GTCGGCAGTG TATCTCTGCG TATGAAACAG 
GTCGAAGAAC TGTATCATTC CCTTCTTGAG CTGGGAGAAA AACGTAAAGG CATGCTAGAA 
AAAAGCTGCA AGAAGTTTAT GCTTTTCCGT GAGGCTAATG AGCTTCAACA GTGGATCAAT 
GAGAAGGAAG CTGCGCTCAC TAATGAGGAA GTGGGTGCTG ATTTGGAGCA GGTGGAGGTG 
CTACAGAAGA AGTTTGATGA TTTTCAGAAG GATTTAAAAG CTAATGAGTC ACGACTGAAG 
GACATAAACA AGGTTGCAAA TGACCTGGAG TCTGAAGGGC TGATGGCAGA GGAAGTGCAA 
GCAGTAGAGC ACCAGGAAGT CTATGGAATG ATGCCCAGAG ATGAAACTGA TTCTAAGACA 
GCCTCTCCTT GGAAGTCTGC ACGTATGATG GTACACACAG TGGCAACGTT TAACTCAATC 
AAGGAACTGA ATGAACGCTG GAGATCCCTG CAGCAGTTGG CAGAGGAGCG AAGCCAGCTG 
TTGGGCAGCG CTCATGAAGT CCAGAGGTTC CACAGAGATG CTGATGAAAC AAAAGAATGG 
ATAGAAGAGA AGAATCAAGC ATTAAATACC GACAACTATG GACATGACTT GGCCAGTGTG 
CAGGCTCTGC AGCGCAAGCA TGAAGGCTTT GAGAGAGACT TGGCAGCTCT TGGAGACAAG 
GTGAATTCTC TTGGTGAAAC TGCCCAGCGT CTGATCCAGT CACATCCAGA ATCAGCTGAA 
GATCTTCAAG AAAAATGTAC TGAGTTGAAC CAAGCCTGGA GTAGTCTGGG AAAACGTGCT 
GACCAACGCA AAGAGAAGCT TGGAGATTCT CATGACCTGC AGCGTTTCCT CAGTGATTTT 
AGAGACCTTA TGTCTTGGAT CAATGGAATC CGGGGTCTGG TCTCCTCAGA TGAACTTGCA 
AAAGATGTGA CTGGAGCTGA AGCTCTGTTG GAAAGGCATC AGGAGCACCG TACTGAAATA 
GATGCACGGG CTGGCACTTT TCAGGCATTT GAACAGTTTG GACAGCAGCT TCTGGCTCGT 
GGACACTATG CCAGCCCAGA GATTAAGGAG AAACTGGATA TTCTAGATCA AGAACGGACA 
GACCTAGAGA AGGCCTGGGT CCAGCGCAGA ATGATGCTAG ACCAGTGCTT AGAACTACAG 
CTGTTTCATC GGGATTGTGA ACAAGCTGAA AACTGGATGG CTGCCCGAGA GGCGTTCCTA 
AATACAGAAG ACAAAGGAGA CTCCTTAGAC AGCGTGGAGG CACTCATCAA GAAGCATGAA 
GATTTCGATA AAGCAATCAA TGTCCAGGAA GAGAAAATTG CTGTCTTGCA GTCTTTTGCT 
GACCAACTGA TTGCTGCTGA TCATTATGCA AAAGGAGTCA TTGCTAACAG ACGCAATGAG 
GTTCTGGACA GGTGGCGTCG TCTGAAGGCT CAGATGATTG AGAAGAGATC TAAGCTGGGA 
GAATCTCAGA CCCTCCAACA GTTCAGTCGT GATGTTGATG AAATAGAAGC TTGGATCAGT 
GAAAAGCTTC AAACTGCAAG TGATGAGTCA TATAAGGATC CCACAAACAT CCAGAGCAAG 
CACCAGAAGC ACCAAGCCTT TGAAGCTGAG CTCCACGCCA ACGCAGATCG GATTCGTGGA 
GTCATTGAAA TGGGGAACTC TCTTATTGAA AGAGGAGCGT GTGCTGGCAG CGAGGATGCT 
GTGAAGGCTC GGCTGGCTGC CCTGGCTGAC CAATGGGAGT TCCTGGTCCA GAAGTCATCA 
GAGAAGAGTC AGAAACTGAA AGAAGCAAAT AAACAGCAGA ATTTCAATAC CGGCATCAAG 
GACTTTGATT TCTGGCTTTC AGAGGTGGAA GCTTTGTTGG CATCTGAAGA CTATGGGAAG 
GACTTGGCAT CAGTGAACAA CCTTCTGAAG AAGCACCAAT TACTGGAAGC TGATATATCT 
GCTCATGAGG ATCGGCTGAA GGACCTGAAC AGCCAGGCTG ACAGTTTGAT GACCAGCAGT 
GCTTTCGATA CCTCCCAAGT AAAGGATAAA CGTGAAACTA TAAATGGGCG CTTCCAGAGA 
ATCAAGAGCA TGGCAGCTGC CCGCCGTGCG AAGCTCAACG AGTCGCACCG CTTGCATCAG 
TTCTTCCGTG ACATGGATGA TGAGGAGTCC TGGATCAAAG AGAAGAAACT GTTGGTGAGC 
TCAGAGGACT ATGGCAGAGA CCTGACTGGT GTGCAGAACC TGAGGAAGAA ACATAAGCGC 
TTGGAAGCAG AATTAGCTGC CCATGAACCT GCTATCCAGG GTGTTCTAGA CACTGGCAAG 
AAGCTTTCAG ATGATAACAC AATTGGGAAG GAGGAGATAC AGCAAAGACT GGCTCAGTTT 
GTGGACCACT GGAAAGAGTT AAAACAGCTG GCAGCTGCTC GGGGGCAGCG TCTGGAGGAG 
TCCCTGGAGT ACCAACAGTT TGTAGCAAAT GTTGAGGAAG AGGAGGCATG GATCAACGAG 
AAAATGACAT TGGTAGCCAG TGAGGATTAT GGAGACACAC TTGCTGCTAT CCAGGGCTTG 
CTGAAGAAGC ATGAAGCATT CGAGACTGAC TTTACTGTCC ACAAAGACAG AGTGAATGAT 
GTTTGCGCTA ATGGAGAGGA TCTCATTAAA AAGAATAATC ACCATGTGGA GAACATTACT 
GCTAAGATGA AGGGCCTCAA GGGCAAGGTA TCAGATCTGG AGAAAGCCGC AGCTCAGAGG 
AAAGCCAAAC TGGATGAGAA CTCTGCCTTC CTCCAGTTCA ACTGGAAAGC AGATGTGGTG 
GAGTCATGGA TAGGTGAGAA GGAAAACAGT CTGAAGACAG ATGATTATGG CCGTGACCTC 
TCCTCTGTGC AAACACTACT CACCAAACAG GAAACCTTTG ATGCTGGACT TCAGGCTTTC 
CAGCAGGAGG GAATTGCAAA CATCACTGCT CTGAAAGACC AGCTACTGGC AGCCAAACAT 
ATCCAATCAA AGGCCATTGA GGTTCGTCAC GCTTCCTTGA TGAAACGCTG GAATCAGCTG 
CTTGCTAATT CTGCAGCCAG GAAAAAGAAA CTCTTGGAGG CTCAGGAGCA CTTCAGAAAG 
GTTGAAGATC TCTTCTTGAC TTTTGCCAAG AAGGCCTCTG CCTTCAACAG TTGGTTTGAG 
AATGCTGAGG AGGACCTGAC GGATCCCGTG CGCTGCAATT CCCTGGAAGA AATCAAAGCA 
CTGCGAGAAG CCCACGATGC GTTCCGTTCC TCACTTAGCT CTGCCCAAGC TGACTTCAAC 
CAGCTGGCAG AGCTTGATCG GCAGATCAAG AGCTTCCGTG TAGCCTCCAA CCCGTACACT 
TGGTTTACTA TGGAGGCTCT TGAAGAAACT TGGAGGAATC TGCAGAAAAT TATCAAGGAG 
CGTGAATTGG AGTTGCAGAA GGAGCAGCGG AGGCAGGAAG AAAATGACAA GTTGCGCCAG 
GAGTTTGCTC AGCATGCTAA TGCCTTCCAC CAGTGGATTC AGGAGACAAG GACTTACCTG 
CTAGATGGGT CCTGCATGGT GGAGGAGTCG GGAACACTGG AATCCCAGCT GGAAGCTACT 
AAACGCAAGC ACCAGGAGAT CCGGGCTATG AGGAGCCAGC TGAAGAAGAT TGAGGACCTT 
GGAGCAGCCA TGGAAGAGGC ACTTATCTTG GACAACAAAT ACACAGAGCA CAGCACCGTG 
GGGCTGGCGC AGCAGTGGGA CCAGCTTGAC CAGCTGGGAA TGAGAATGCA GCACAATCTG 
GAACAGCAGA TTCAAGCTCG AAACACAACT GGAGTCACAG AGGAGGCCCT GAAGGAGTTC 
AGCATGATGT TCAAGCACTT TGACAAGGAC AAATCTGGAC GTCTTAATCA CCAGGAGTTT 
AAGTCTTGCT TGCGCTCCCT TGGCTATGAC CTGCCCATGG TTGAGGAAGG AGAGCCTGAC 
CCCGAATTTG AGTCTATTCT TGACACTGTT GATCCCAACA GGGATGGCCA CGTCTCGCTG 
CAGGAGTACA TGGCGTTCAT GATCAGCAGG GAAACAGAGA ACGTGAAATC CAGCGAGGAG 
ATTGAGAGTG CTTTCCGTGC CCTCAGCTCG GAGGGGAAGC CTTACGTGAC CAAGGAGGAG 
CTCTACCAGA ACCTGACCCG GGAGCAGGCC GACTACTGCA TTTCTCACAT GAAGCCCTAC 
ATGGATGGCA AGGGCAGAGA ACTTCCTTCT GCCTACGACT ACATAGAATT TACACGTTCA 
CTCTTTGTGA ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_015279292.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015134778.1 Gallus gallus spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X11, mRNA.

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CloneID	OGa43318
Clone ID Related Accession (Same CDS sequence)	XM_015279292.2 , XM_015279292.1
Accession Version	XM_015279292.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7455bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG 
TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC 
TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT 
GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT 
GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG 
ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAA CTCGTCTACA GGAGCTGCAC 
CGACTATGGG AGTTACTGCT GGAAAAAATG AGAGAGAAGG GAGTGAAGCT GTTGCAAGCA 
CAGAAGCTGG TGCAATTCTT ACGGGAATGT GAAGATGTCA TGGACTGGAT CAATGACAAG 
GAAGCAATAG TGACCTCAGA AGAGCTTGGA CAGGACTTAG AGCATGTTGA GGTTTTGCAG 
AAGAAATTTG AAGAGTTCCA AACAGATCTG GCAGCTCATG AGGAGAGAGT GAATGAAGTG 
AACCAGTTCG CTGGCAAACT TATCCAGGAA CAGCACCCTG AGGAGGAACT TATAAAGTCC 
AAACAGGATG AAGTAAATGC AAGCTGGCAG CGTCTTAAGG GGCTTGCCCA ACAGAGGCAA 
GGAAAACTCT TTGGGGCAGC TGAAGTTCAG CGCTTCAACA GGGATGTGGA TGAAACTATC 
AGCTGGATTA AGGAGAAAGG GCAGTTGATG GCCTCAGATG ACTTTGGCAG AGACTTGGCC 
AGCGTGCAAG CTTTACTACG TAAGCATGAA GGCCTGGAAA GAGATCTTGC AGCTTTGGAG 
GATAAGGTTA AGGCCCTCTG TGCAGAAGCT GACCGTTTGC AGCAGTCTCA CCCTATAAAT 
GCTTCTCAAA TTCAAGTGAA ACGGGAGGAA CTGATTGCCA ACTGGGAGCA GATCCGAACT 
CTGGCAGCAG AGAGGCATGC TCGCCTTAAT GACTCCTACA GGTTGCAGCG CTTTCTTGCA 
GATTTCCGGG ACCTCACTAG CTGGGTAACT GAGATGAAGG CTCTGATAAA TGCTGATGAG 
CTTGCCAATG ATGTGGCTGG AGCAGAAGCC CTTCTAGATA GACATCAGGA ACATAAGGGA 
GAAATTGATG CTCATGAGGA TAGCTTCAGA TCTGCTGATG AGTCTGGGCA GGCTTTGCTT 
GCAGCTGGGC ACTATGCTTC TGATGAAGTT AAAGAAAAGC TGACTATCCT CTCAGATGAA 
AGATCAGCCT TGCTGGAACT ATGGGAGCTT CGCAGGCAAC AGTATGAGCA GTGCATGGAT 
CTGCAGCTTT TCTACAGAGA TACTGAACAA GTTGACAACT GGATGAGCAA ACAAGAAGCT 
TTTCTGCTGA ACGAAGACCT TGGTGATTCT CTGGATAGCG TGGAGGCTCT TCTAAAGAAG 
CATGAAGATT TTGAGAAATC CCTAAGTGCT CAAGAGGAGA AGATCACAGC ACTGGATGAG 
TTTGCTACTA AACTGATTCA AAATAACCAT TATGCCATGG ATGACGTTGC TACGCGCAGA 
GATGCTCTTC TGAGTCGCCG AAATGCTCTT CATGAAAGAG CCATGAAGCG CCGTGCCCAA 
CTGGCAGACT CTTTCCATCT CCAGCAGTTT TTCAGGGACT CTGATGAACT GAAGAGTTGG 
GTTAATGAAA AAATGAAAAC TGCTACCGAT GAGGCTTACA AGGATCCATC CAACTTGCAA 
GGTAAAGTTC AGAAGCATCA GGCTTTTGAA GCAGAGCTTT CTGCTAATCA GAGTCGTATT 
GATGCACTGG AGAAAGCTGG CCAGAAGTTG ATTGATGTCA ATCACTATGC ATCTGATGAG 
GTGGCAGCTC GCATGAATGA AGTCATCAGC TTGTGGAAGA AACTTCTGGA AGCCACCGAG 
CTCAAAGGTA TAAAGCTGCG TGAAGCCAAT CAACAGCAGC AATTTAATCG CAATGTAGAA 
GACATTGAGT TGTGGCTGTA TGAAGTGGAG GGTCACTTGG CTTCTGATGA TTATGGAAAA 
GATCTTACCA GCGTTCAGAA TCTTCAGAAG AAACATGCCC TGCTAGAGGC AGATGTTGCT 
GCCCATCAGG ATCGGATAGA TGGCATTACC ATCCAGGCAC GCCAGTTCCA AGATGCTGGG 
CACTTTGATG CTGACAATAT CAAGAAGAAA CAAGAAGCTT TAGTAGCTCG TTATGAAGCT 
CTGAAGGATC CTATGGTGGC TCGCAAGCAG AAACTTGCAG ATTCTCTTCG CCTGCAGCAG 
CTTTTCCGTG ACATCGAGGA TGAAGAGACC TGGATCAGGG AAAAGGAACC TATTGCAGCC 
TCAACAAACC GAGGCAAGGA CTTAATTGGT GTCCAGAATC TGCTAAAGAA GCACCAGGCT 
TTGCAGGCAG AAATTGCAGG CCATGAGCCT CGCATTAAAG CAGTCACACA AAAAGGAAAT 
GCGATGGTGG AAGAGGGACA TTTTGCTGCT GAAGATGTGA AGATCAAATT GAATGAACTA 
AACCAAAAAT GGGACTCTCT GAAAGCCAAG GCATCTCAAC GGCGACAGGA TCTAGAGGAT 
TCTCTGCAAG CTCAGCAGTA TTTTGCTGAT GCTAATGAGG CAGAATCATG GATGAGGGAA 
AAGGAGCCCA TTGTAGGCAG CACGGATTAT GGAAAGGATG AAGACTCTGC TGAGGCTCTC 
CTGAAGAAGC ATGAAGCTTT GATGTCTGAT CTTTCTGCTT ACGGCAGTAG CATACAGGCA 
TTAAGGGAAC AGGCCCAGTC CTGCAGGCAA CAAGTTGCTC CCACTGATGA TGAAACTGGA 
AAAGAGCTTG TGCTAGCACT CTATGATTAC CAAGAGAAGA GTCCTCGGGA GGTGACTATG 
AAGAAAGGAG ATATTCTAAC CCTGCTCAAC AGCACCAACA AGGACTGGTG GAAGGTTGAA 
GTTAACGATC GTCAGGGCTT TGTACCAGCT GCCTATGTGA AAAAACTAGA TCCTGCCCAG 
TCTGCATCCC GAGAGAATCT TCTAGAAGAG CAAGGCAGCA TAGCATTGCG ACAGGAGCAA 
ATTGACAACC AGACTCTCAT TACTAAGGAA GTCGGCAGTG TATCTCTGCG TATGAAACAG 
GTCGAAGAAC TGTATCATTC CCTTCTTGAG CTGGGAGAAA AACGTAAAGG CATGCTAGAA 
AAAAGCTGCA AGAAGTTTAT GCTTTTCCGT GAGGCTAATG AGCTTCAACA GTGGATCAAT 
GAGAAGGAAG CTGCGCTCAC TAATGAGGAA GTGGGTGCTG ATTTGGAGCA GGTGGAGGTG 
CTACAGAAGA AGTTTGATGA TTTTCAGAAG GATTTAAAAG CTAATGAGTC ACGACTGAAG 
GACATAAACA AGGTTGCAAA TGACCTGGAG TCTGAAGGGC TGATGGCAGA GGAAGTGCAA 
GCAGTAGAGC ACCAGGAAGT CTATGGAATG ATGCCCAGAG ATGAAACTGA TTCTAAGACA 
GCCTCTCCTT GGAAGTCTGC ACGTATGATG GTACACACAG TGGCAACGTT TAACTCAATC 
AAGGAACTGA ATGAACGCTG GAGATCCCTG CAGCAGTTGG CAGAGGAGCG AAGCCAGCTG 
TTGGGCAGCG CTCATGAAGT CCAGAGGTTC CACAGAGATG CTGATGAAAC AAAAGAATGG 
ATAGAAGAGA AGAATCAAGC ATTAAATACC GACAACTATG GACATGACTT GGCCAGTGTG 
CAGGCTCTGC AGCGCAAGCA TGAAGGCTTT GAGAGAGACT TGGCAGCTCT TGGAGACAAG 
GTGAATTCTC TTGGTGAAAC TGCCCAGCGT CTGATCCAGT CACATCCAGA ATCAGCTGAA 
GATCTTCAAG AAAAATGTAC TGAGTTGAAC CAAGCCTGGA GTAGTCTGGG AAAACGTGCT 
GACCAACGCA AAGAGAAGCT TGGAGATTCT CATGACCTGC AGCGTTTCCT CAGTGATTTT 
AGAGACCTTA TGTCTTGGAT CAATGGAATC CGGGGTCTGG TCTCCTCAGA TGAACTTGCA 
AAAGATGTGA CTGGAGCTGA AGCTCTGTTG GAAAGGCATC AGGAGCACCG TACTGAAATA 
GATGCACGGG CTGGCACTTT TCAGGCATTT GAACAGTTTG GACAGCAGCT TCTGGCTCGT 
GGACACTATG CCAGCCCAGA GATTAAGGAG AAACTGGATA TTCTAGATCA AGAACGGACA 
GACCTAGAGA AGGCCTGGGT CCAGCGCAGA ATGATGCTAG ACCAGTGCTT AGAACTACAG 
CTGTTTCATC GGGATTGTGA ACAAGCTGAA AACTGGATGG CTGCCCGAGA GGCGTTCCTA 
AATACAGAAG ACAAAGGAGA CTCCTTAGAC AGCGTGGAGG CACTCATCAA GAAGCATGAA 
GATTTCGATA AAGCAATCAA TGTCCAGGAA GAGAAAATTG CTGTCTTGCA GTCTTTTGCT 
GACCAACTGA TTGCTGCTGA TCATTATGCA AAAGGAGTCA TTGCTAACAG ACGCAATGAG 
GTTCTGGACA GGTGGCGTCG TCTGAAGGCT CAGATGATTG AGAAGAGATC TAAGCTGGGA 
GAATCTCAGA CCCTCCAACA GTTCAGTCGT GATGTTGATG AAATAGAAGC TTGGATCAGT 
GAAAAGCTTC AAACTGCAAG TGATGAGTCA TATAAGGATC CCACAAACAT CCAGAGCAAG 
CACCAGAAGC ACCAAGCCTT TGAAGCTGAG CTCCACGCCA ACGCAGATCG GATTCGTGGA 
GTCATTGAAA TGGGGAACTC TCTTATTGAA AGAGGAGCGT GTGCTGGCAG CGAGGATGCT 
GTGAAGGCTC GGCTGGCTGC CCTGGCTGAC CAATGGGAGT TCCTGGTCCA GAAGTCATCA 
GAGAAGAGTC AGAAACTGAA AGAAGCAAAT AAACAGCAGA ATTTCAATAC CGGCATCAAG 
GACTTTGATT TCTGGCTTTC AGAGGTGGAA GCTTTGTTGG CATCTGAAGA CTATGGGAAG 
GACTTGGCAT CAGTGAACAA CCTTCTGAAG AAGCACCAAT TACTGGAAGC TGATATATCT 
GCTCATGAGG ATCGGCTGAA GGACCTGAAC AGCCAGGCTG ACAGTTTGAT GACCAGCAGT 
GCTTTCGATA CCTCCCAAGT AAAGGATAAA CGTGAAACTA TAAATGGGCG CTTCCAGAGA 
ATCAAGAGCA TGGCAGCTGC CCGCCGTGCG AAGCTCAACG AGTCGCACCG CTTGCATCAG 
TTCTTCCGTG ACATGGATGA TGAGGAGTCC TGGATCAAAG AGAAGAAACT GTTGGTGAGC 
TCAGAGGACT ATGGCAGAGA CCTGACTGGT GTGCAGAACC TGAGGAAGAA ACATAAGCGC 
TTGGAAGCAG AATTAGCTGC CCATGAACCT GCTATCCAGG GTGTTCTAGA CACTGGCAAG 
AAGCTTTCAG ATGATAACAC AATTGGGAAG GAGGAGATAC AGCAAAGACT GGCTCAGTTT 
GTGGACCACT GGAAAGAGTT AAAACAGCTG GCAGCTGCTC GGGGGCAGCG TCTGGAGGAG 
TCCCTGGAGT ACCAACAGTT TGTAGCAAAT GTTGAGGAAG AGGAGGCATG GATCAACGAG 
AAAATGACAT TGGTAGCCAG TGAGGATTAT GGAGACACAC TTGCTGCTAT CCAGGGCTTG 
CTGAAGAAGC ATGAAGCATT CGAGACTGAC TTTACTGTCC ACAAAGACAG AGTGAATGAT 
GTTTGCGCTA ATGGAGAGGA TCTCATTAAA AAGAATAATC ACCATGTGGA GAACATTACT 
GCTAAGATGA AGGGCCTCAA GGGCAAGGTA TCAGATCTGG AGAAAGCCGC AGCTCAGAGG 
AAAGCCAAAC TGGATGAGAA CTCTGCCTTC CTCCAGTTCA ACTGGAAAGC AGATGTGGTG 
GAGTCATGGA TAGGTGAGAA GGAAAACAGT CTGAAGACAG ATGATTATGG CCGTGACCTC 
TCCTCTGTGC AAACACTACT CACCAAACAG GAAACCTTTG ATGCTGGACT TCAGGCTTTC 
CAGCAGGAGG GAATTGCAAA CATCACTGCT CTGAAAGACC AGCTACTGGC AGCCAAACAT 
ATCCAATCAA AGGCCATTGA GGTTCGTCAC GCTTCCTTGA TGAAACGCTG GAATCAGCTG 
CTTGCTAATT CTGCAGCCAG GAAAAAGAAA CTCTTGGAGG CTCAGGAGCA CTTCAGAAAG 
GTTGAAGATC TCTTCTTGAC TTTTGCCAAG AAGGCCTCTG CCTTCAACAG TTGGTTTGAG 
AATGCTGAGG AGGACCTGAC GGATCCCGTG CGCTGCAATT CCCTGGAAGA AATCAAAGCA 
CTGCGAGAAG CCCACGATGC GTTCCGTTCC TCACTTAGCT CTGCCCAAGC TGACTTCAAC 
CAGCTGGCAG AGCTTGATCG GCAGATCAAG AGCTTCCGTG TAGCCTCCAA CCCGTACACT 
TGGTTTACTA TGGAGGCTCT TGAAGAAACT TGGAGGAATC TGCAGAAAAT TATCAAGGAG 
CGTGAATTGG AGTTGCAGAA GGAGCAGCGG AGGCAGGAAG AAAATGACAA GTTGCGCCAG 
GAGTTTGCTC AGCATGCTAA TGCCTTCCAC CAGTGGATTC AGGAGACAAG GACTTACCTG 
CTAGATGGGT CCTGCATGGT GGAGGAGTCG GGAACACTGG AATCCCAGCT GGAAGCTACT 
AAACGCAAGC ACCAGGAGAT CCGGGCTATG AGGAGCCAGC TGAAGAAGAT TGAGGACCTT 
GGAGCAGCCA TGGAAGAGGC ACTTATCTTG GACAACAAAT ACACAGAGCA CAGCACCGTG 
GGGCTGGCGC AGCAGTGGGA CCAGCTTGAC CAGCTGGGAA TGAGAATGCA GCACAATCTG 
GAACAGCAGA TTCAAGCTCG AAACACAACT GGAGTCACAG AGGAGGCCCT GAAGGAGTTC 
AGCATGATGT TCAAGCACTT TGACAAGGAC AAATCTGGAC GTCTTAATCA CCAGGAGTTT 
AAGTCTTGCT TGCGCTCCCT TGGCTATGAC CTGCCCATGG TTGAGGAAGG AGAGCCTGAC 
CCCGAATTTG AGTCTATTCT TGACACTGTT GATCCCAACA GGGATGGCCA CGTCTCGCTG 
CAGGAGTACA TGGCGTTCAT GATCAGCAGG GAAACAGAGA ACGTGAAATC CAGCGAGGAG 
ATTGAGAGTG CTTTCCGTGC CCTCAGCTCG GAGGGGAAGC CTTACGTGAC CAAGGAGGAG 
CTCTACCAGA ACCTGACCCG GGAGCAGGCC GACTACTGCA TTTCTCACAT GAAGCCCTAC 
ATGGATGGCA AGGGCAGAGA ACTTCCTTCT GCCTACGACT ACATAGAATT TACACGTTCA 
CTCTTTGTGA ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015134778.1
CDS	10..7464
Translation	MLSSFRKVKVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSSLRRQKLEDSYRFQFFQRDADELEKWIQEKLQIASDENYKDPSNLQGKLQKHQAFEAEVQANSGAIVKLDETGNQMINEGHFASETIRTRLQELHRLWELLLEKMREKGVKLLQAQKLVQFLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDLAAHEERVNEVNQFAGKLIQEQHPEEELIKSKQDEVNASWQRLKGLAQQRQGKLFGAAEVQRFNRDVDETISWIKEKGQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPINASQIQVKREELIANWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFRSADESGQALLAAGHYASDEVKEKLTILSDERSALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMDDVATRRDALLSRRNALHERAMKRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYASDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTSVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDADNIKKKQEALVARYEALKDPMVARKQKLADSLRLQQLFRDIEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKIKLNELNQKWDSLKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPTDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTLITKEVGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTNEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVANDLESEGLMAEEVQAVEHQEVYGMMPRDETDSKTASPWKSARMMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAQRLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKEKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLARGHYASPEIKEKLDILDQERTDLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAVLQSFADQLIAADHYAKGVIANRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQSKHQKHQAFEAELHANADRIRGVIEMGNSLIERGACAGSEDAVKARLAALADQWEFLVQKSSEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRETINGRFQRIKSMAAARRAKLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVDHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCANGEDLIKKNNHHVENITAKMKGLKGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHIQSKAIEVRHASLMKRWNQLLANSAARKKKLLEAQEHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFESILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCISHMKPYMDGKGRELPSAYDYIEFTRSLFVN

Target ORF information:

RefSeq Version	XM_015279292.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279292.1

ORF Insert Sequence:

ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG 
TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC 
TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT 
GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT 
GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG 
ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAA CTCGTCTACA GGAGCTGCAC 
CGACTATGGG AGTTACTGCT GGAAAAAATG AGAGAGAAGG GAGTGAAGCT GTTGCAAGCA 
CAGAAGCTGG TGCAATTCTT ACGGGAATGT GAAGATGTCA TGGACTGGAT CAATGACAAG 
GAAGCAATAG TGACCTCAGA AGAGCTTGGA CAGGACTTAG AGCATGTTGA GGTTTTGCAG 
AAGAAATTTG AAGAGTTCCA AACAGATCTG GCAGCTCATG AGGAGAGAGT GAATGAAGTG 
AACCAGTTCG CTGGCAAACT TATCCAGGAA CAGCACCCTG AGGAGGAACT TATAAAGTCC 
AAACAGGATG AAGTAAATGC AAGCTGGCAG CGTCTTAAGG GGCTTGCCCA ACAGAGGCAA 
GGAAAACTCT TTGGGGCAGC TGAAGTTCAG CGCTTCAACA GGGATGTGGA TGAAACTATC 
AGCTGGATTA AGGAGAAAGG GCAGTTGATG GCCTCAGATG ACTTTGGCAG AGACTTGGCC 
AGCGTGCAAG CTTTACTACG TAAGCATGAA GGCCTGGAAA GAGATCTTGC AGCTTTGGAG 
GATAAGGTTA AGGCCCTCTG TGCAGAAGCT GACCGTTTGC AGCAGTCTCA CCCTATAAAT 
GCTTCTCAAA TTCAAGTGAA ACGGGAGGAA CTGATTGCCA ACTGGGAGCA GATCCGAACT 
CTGGCAGCAG AGAGGCATGC TCGCCTTAAT GACTCCTACA GGTTGCAGCG CTTTCTTGCA 
GATTTCCGGG ACCTCACTAG CTGGGTAACT GAGATGAAGG CTCTGATAAA TGCTGATGAG 
CTTGCCAATG ATGTGGCTGG AGCAGAAGCC CTTCTAGATA GACATCAGGA ACATAAGGGA 
GAAATTGATG CTCATGAGGA TAGCTTCAGA TCTGCTGATG AGTCTGGGCA GGCTTTGCTT 
GCAGCTGGGC ACTATGCTTC TGATGAAGTT AAAGAAAAGC TGACTATCCT CTCAGATGAA 
AGATCAGCCT TGCTGGAACT ATGGGAGCTT CGCAGGCAAC AGTATGAGCA GTGCATGGAT 
CTGCAGCTTT TCTACAGAGA TACTGAACAA GTTGACAACT GGATGAGCAA ACAAGAAGCT 
TTTCTGCTGA ACGAAGACCT TGGTGATTCT CTGGATAGCG TGGAGGCTCT TCTAAAGAAG 
CATGAAGATT TTGAGAAATC CCTAAGTGCT CAAGAGGAGA AGATCACAGC ACTGGATGAG 
TTTGCTACTA AACTGATTCA AAATAACCAT TATGCCATGG ATGACGTTGC TACGCGCAGA 
GATGCTCTTC TGAGTCGCCG AAATGCTCTT CATGAAAGAG CCATGAAGCG CCGTGCCCAA 
CTGGCAGACT CTTTCCATCT CCAGCAGTTT TTCAGGGACT CTGATGAACT GAAGAGTTGG 
GTTAATGAAA AAATGAAAAC TGCTACCGAT GAGGCTTACA AGGATCCATC CAACTTGCAA 
GGTAAAGTTC AGAAGCATCA GGCTTTTGAA GCAGAGCTTT CTGCTAATCA GAGTCGTATT 
GATGCACTGG AGAAAGCTGG CCAGAAGTTG ATTGATGTCA ATCACTATGC ATCTGATGAG 
GTGGCAGCTC GCATGAATGA AGTCATCAGC TTGTGGAAGA AACTTCTGGA AGCCACCGAG 
CTCAAAGGTA TAAAGCTGCG TGAAGCCAAT CAACAGCAGC AATTTAATCG CAATGTAGAA 
GACATTGAGT TGTGGCTGTA TGAAGTGGAG GGTCACTTGG CTTCTGATGA TTATGGAAAA 
GATCTTACCA GCGTTCAGAA TCTTCAGAAG AAACATGCCC TGCTAGAGGC AGATGTTGCT 
GCCCATCAGG ATCGGATAGA TGGCATTACC ATCCAGGCAC GCCAGTTCCA AGATGCTGGG 
CACTTTGATG CTGACAATAT CAAGAAGAAA CAAGAAGCTT TAGTAGCTCG TTATGAAGCT 
CTGAAGGATC CTATGGTGGC TCGCAAGCAG AAACTTGCAG ATTCTCTTCG CCTGCAGCAG 
CTTTTCCGTG ACATCGAGGA TGAAGAGACC TGGATCAGGG AAAAGGAACC TATTGCAGCC 
TCAACAAACC GAGGCAAGGA CTTAATTGGT GTCCAGAATC TGCTAAAGAA GCACCAGGCT 
TTGCAGGCAG AAATTGCAGG CCATGAGCCT CGCATTAAAG CAGTCACACA AAAAGGAAAT 
GCGATGGTGG AAGAGGGACA TTTTGCTGCT GAAGATGTGA AGATCAAATT GAATGAACTA 
AACCAAAAAT GGGACTCTCT GAAAGCCAAG GCATCTCAAC GGCGACAGGA TCTAGAGGAT 
TCTCTGCAAG CTCAGCAGTA TTTTGCTGAT GCTAATGAGG CAGAATCATG GATGAGGGAA 
AAGGAGCCCA TTGTAGGCAG CACGGATTAT GGAAAGGATG AAGACTCTGC TGAGGCTCTC 
CTGAAGAAGC ATGAAGCTTT GATGTCTGAT CTTTCTGCTT ACGGCAGTAG CATACAGGCA 
TTAAGGGAAC AGGCCCAGTC CTGCAGGCAA CAAGTTGCTC CCACTGATGA TGAAACTGGA 
AAAGAGCTTG TGCTAGCACT CTATGATTAC CAAGAGAAGA GTCCTCGGGA GGTGACTATG 
AAGAAAGGAG ATATTCTAAC CCTGCTCAAC AGCACCAACA AGGACTGGTG GAAGGTTGAA 
GTTAACGATC GTCAGGGCTT TGTACCAGCT GCCTATGTGA AAAAACTAGA TCCTGCCCAG 
TCTGCATCCC GAGAGAATCT TCTAGAAGAG CAAGGCAGCA TAGCATTGCG ACAGGAGCAA 
ATTGACAACC AGACTCTCAT TACTAAGGAA GTCGGCAGTG TATCTCTGCG TATGAAACAG 
GTCGAAGAAC TGTATCATTC CCTTCTTGAG CTGGGAGAAA AACGTAAAGG CATGCTAGAA 
AAAAGCTGCA AGAAGTTTAT GCTTTTCCGT GAGGCTAATG AGCTTCAACA GTGGATCAAT 
GAGAAGGAAG CTGCGCTCAC TAATGAGGAA GTGGGTGCTG ATTTGGAGCA GGTGGAGGTG 
CTACAGAAGA AGTTTGATGA TTTTCAGAAG GATTTAAAAG CTAATGAGTC ACGACTGAAG 
GACATAAACA AGGTTGCAAA TGACCTGGAG TCTGAAGGGC TGATGGCAGA GGAAGTGCAA 
GCAGTAGAGC ACCAGGAAGT CTATGGAATG ATGCCCAGAG ATGAAACTGA TTCTAAGACA 
GCCTCTCCTT GGAAGTCTGC ACGTATGATG GTACACACAG TGGCAACGTT TAACTCAATC 
AAGGAACTGA ATGAACGCTG GAGATCCCTG CAGCAGTTGG CAGAGGAGCG AAGCCAGCTG 
TTGGGCAGCG CTCATGAAGT CCAGAGGTTC CACAGAGATG CTGATGAAAC AAAAGAATGG 
ATAGAAGAGA AGAATCAAGC ATTAAATACC GACAACTATG GACATGACTT GGCCAGTGTG 
CAGGCTCTGC AGCGCAAGCA TGAAGGCTTT GAGAGAGACT TGGCAGCTCT TGGAGACAAG 
GTGAATTCTC TTGGTGAAAC TGCCCAGCGT CTGATCCAGT CACATCCAGA ATCAGCTGAA 
GATCTTCAAG AAAAATGTAC TGAGTTGAAC CAAGCCTGGA GTAGTCTGGG AAAACGTGCT 
GACCAACGCA AAGAGAAGCT TGGAGATTCT CATGACCTGC AGCGTTTCCT CAGTGATTTT 
AGAGACCTTA TGTCTTGGAT CAATGGAATC CGGGGTCTGG TCTCCTCAGA TGAACTTGCA 
AAAGATGTGA CTGGAGCTGA AGCTCTGTTG GAAAGGCATC AGGAGCACCG TACTGAAATA 
GATGCACGGG CTGGCACTTT TCAGGCATTT GAACAGTTTG GACAGCAGCT TCTGGCTCGT 
GGACACTATG CCAGCCCAGA GATTAAGGAG AAACTGGATA TTCTAGATCA AGAACGGACA 
GACCTAGAGA AGGCCTGGGT CCAGCGCAGA ATGATGCTAG ACCAGTGCTT AGAACTACAG 
CTGTTTCATC GGGATTGTGA ACAAGCTGAA AACTGGATGG CTGCCCGAGA GGCGTTCCTA 
AATACAGAAG ACAAAGGAGA CTCCTTAGAC AGCGTGGAGG CACTCATCAA GAAGCATGAA 
GATTTCGATA AAGCAATCAA TGTCCAGGAA GAGAAAATTG CTGTCTTGCA GTCTTTTGCT 
GACCAACTGA TTGCTGCTGA TCATTATGCA AAAGGAGTCA TTGCTAACAG ACGCAATGAG 
GTTCTGGACA GGTGGCGTCG TCTGAAGGCT CAGATGATTG AGAAGAGATC TAAGCTGGGA 
GAATCTCAGA CCCTCCAACA GTTCAGTCGT GATGTTGATG AAATAGAAGC TTGGATCAGT 
GAAAAGCTTC AAACTGCAAG TGATGAGTCA TATAAGGATC CCACAAACAT CCAGAGCAAG 
CACCAGAAGC ACCAAGCCTT TGAAGCTGAG CTCCACGCCA ACGCAGATCG GATTCGTGGA 
GTCATTGAAA TGGGGAACTC TCTTATTGAA AGAGGAGCGT GTGCTGGCAG CGAGGATGCT 
GTGAAGGCTC GGCTGGCTGC CCTGGCTGAC CAATGGGAGT TCCTGGTCCA GAAGTCATCA 
GAGAAGAGTC AGAAACTGAA AGAAGCAAAT AAACAGCAGA ATTTCAATAC CGGCATCAAG 
GACTTTGATT TCTGGCTTTC AGAGGTGGAA GCTTTGTTGG CATCTGAAGA CTATGGGAAG 
GACTTGGCAT CAGTGAACAA CCTTCTGAAG AAGCACCAAT TACTGGAAGC TGATATATCT 
GCTCATGAGG ATCGGCTGAA GGACCTGAAC AGCCAGGCTG ACAGTTTGAT GACCAGCAGT 
GCTTTCGATA CCTCCCAAGT AAAGGATAAA CGTGAAACTA TAAATGGGCG CTTCCAGAGA 
ATCAAGAGCA TGGCAGCTGC CCGCCGTGCG AAGCTCAACG AGTCGCACCG CTTGCATCAG 
TTCTTCCGTG ACATGGATGA TGAGGAGTCC TGGATCAAAG AGAAGAAACT GTTGGTGAGC 
TCAGAGGACT ATGGCAGAGA CCTGACTGGT GTGCAGAACC TGAGGAAGAA ACATAAGCGC 
TTGGAAGCAG AATTAGCTGC CCATGAACCT GCTATCCAGG GTGTTCTAGA CACTGGCAAG 
AAGCTTTCAG ATGATAACAC AATTGGGAAG GAGGAGATAC AGCAAAGACT GGCTCAGTTT 
GTGGACCACT GGAAAGAGTT AAAACAGCTG GCAGCTGCTC GGGGGCAGCG TCTGGAGGAG 
TCCCTGGAGT ACCAACAGTT TGTAGCAAAT GTTGAGGAAG AGGAGGCATG GATCAACGAG 
AAAATGACAT TGGTAGCCAG TGAGGATTAT GGAGACACAC TTGCTGCTAT CCAGGGCTTG 
CTGAAGAAGC ATGAAGCATT CGAGACTGAC TTTACTGTCC ACAAAGACAG AGTGAATGAT 
GTTTGCGCTA ATGGAGAGGA TCTCATTAAA AAGAATAATC ACCATGTGGA GAACATTACT 
GCTAAGATGA AGGGCCTCAA GGGCAAGGTA TCAGATCTGG AGAAAGCCGC AGCTCAGAGG 
AAAGCCAAAC TGGATGAGAA CTCTGCCTTC CTCCAGTTCA ACTGGAAAGC AGATGTGGTG 
GAGTCATGGA TAGGTGAGAA GGAAAACAGT CTGAAGACAG ATGATTATGG CCGTGACCTC 
TCCTCTGTGC AAACACTACT CACCAAACAG GAAACCTTTG ATGCTGGACT TCAGGCTTTC 
CAGCAGGAGG GAATTGCAAA CATCACTGCT CTGAAAGACC AGCTACTGGC AGCCAAACAT 
ATCCAATCAA AGGCCATTGA GGTTCGTCAC GCTTCCTTGA TGAAACGCTG GAATCAGCTG 
CTTGCTAATT CTGCAGCCAG GAAAAAGAAA CTCTTGGAGG CTCAGGAGCA CTTCAGAAAG 
GTTGAAGATC TCTTCTTGAC TTTTGCCAAG AAGGCCTCTG CCTTCAACAG TTGGTTTGAG 
AATGCTGAGG AGGACCTGAC GGATCCCGTG CGCTGCAATT CCCTGGAAGA AATCAAAGCA 
CTGCGAGAAG CCCACGATGC GTTCCGTTCC TCACTTAGCT CTGCCCAAGC TGACTTCAAC 
CAGCTGGCAG AGCTTGATCG GCAGATCAAG AGCTTCCGTG TAGCCTCCAA CCCGTACACT 
TGGTTTACTA TGGAGGCTCT TGAAGAAACT TGGAGGAATC TGCAGAAAAT TATCAAGGAG 
CGTGAATTGG AGTTGCAGAA GGAGCAGCGG AGGCAGGAAG AAAATGACAA GTTGCGCCAG 
GAGTTTGCTC AGCATGCTAA TGCCTTCCAC CAGTGGATTC AGGAGACAAG GACTTACCTG 
CTAGATGGGT CCTGCATGGT GGAGGAGTCG GGAACACTGG AATCCCAGCT GGAAGCTACT 
AAACGCAAGC ACCAGGAGAT CCGGGCTATG AGGAGCCAGC TGAAGAAGAT TGAGGACCTT 
GGAGCAGCCA TGGAAGAGGC ACTTATCTTG GACAACAAAT ACACAGAGCA CAGCACCGTG 
GGGCTGGCGC AGCAGTGGGA CCAGCTTGAC CAGCTGGGAA TGAGAATGCA GCACAATCTG 
GAACAGCAGA TTCAAGCTCG AAACACAACT GGAGTCACAG AGGAGGCCCT GAAGGAGTTC 
AGCATGATGT TCAAGCACTT TGACAAGGAC AAATCTGGAC GTCTTAATCA CCAGGAGTTT 
AAGTCTTGCT TGCGCTCCCT TGGCTATGAC CTGCCCATGG TTGAGGAAGG AGAGCCTGAC 
CCCGAATTTG AGTCTATTCT TGACACTGTT GATCCCAACA GGGATGGCCA CGTCTCGCTG 
CAGGAGTACA TGGCGTTCAT GATCAGCAGG GAAACAGAGA ACGTGAAATC CAGCGAGGAG 
ATTGAGAGTG CTTTCCGTGC CCTCAGCTCG GAGGGGAAGC CTTACGTGAC CAAGGAGGAG 
CTCTACCAGA ACCTGACCCG GGAGCAGGCC GACTACTGCA TTTCTCACAT GAAGCCCTAC 
ATGGATGGCA AGGGCAGAGA ACTTCCTTCT GCCTACGACT ACATAGAATT TACACGTTCA 
CTCTTTGTGA ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_015279293.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015134779.1 Gallus gallus spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X12, mRNA.

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CloneID	OGa43319
Clone ID Related Accession (Same CDS sequence)	XM_015279293.1 , XM_015279293.2
Accession Version	XM_015279293.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7410bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG 
TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC 
TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT 
GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT 
GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG 
ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAA CTCGTCTACA GGAGCTGCAC 
CGACTATGGG AGTTACTGCT GGAAAAAATG AGAGAGAAGG GAGTGAAGCT GTTGCAAGCA 
CAGAAGCTGG TGCAATTCTT ACGGGAATGT GAAGATGTCA TGGACTGGAT CAATGACAAG 
GAAGCAATAG TGACCTCAGA AGAGCTTGGA CAGGACTTAG AGCATGTTGA GGTTTTGCAG 
AAGAAATTTG AAGAGTTCCA AACAGATCTG GCAGCTCATG AGGAGAGAGT GAATGAAGTG 
AACCAGTTCG CTGGCAAACT TATCCAGGAA CAGCACCCTG AGGAGGAACT TATAAAGTCC 
AAACAGGATG AAGTAAATGC AAGCTGGCAG CGTCTTAAGG GGCTTGCCCA ACAGAGGCAA 
GGAAAACTCT TTGGGGCAGC TGAAGTTCAG CGCTTCAACA GGGATGTGGA TGAAACTATC 
AGCTGGATTA AGGAGAAAGG GCAGTTGATG GCCTCAGATG ACTTTGGCAG AGACTTGGCC 
AGCGTGCAAG CTTTACTACG TAAGCATGAA GGCCTGGAAA GAGATCTTGC AGCTTTGGAG 
GATAAGGTTA AGGCCCTCTG TGCAGAAGCT GACCGTTTGC AGCAGTCTCA CCCTATAAAT 
GCTTCTCAAA TTCAAGTGAA ACGGGAGGAA CTGATTGCCA ACTGGGAGCA GATCCGAACT 
CTGGCAGCAG AGAGGCATGC TCGCCTTAAT GACTCCTACA GGTTGCAGCG CTTTCTTGCA 
GATTTCCGGG ACCTCACTAG CTGGGTAACT GAGATGAAGG CTCTGATAAA TGCTGATGAG 
CTTGCCAATG ATGTGGCTGG AGCAGAAGCC CTTCTAGATA GACATCAGGA ACATAAGGGA 
GAAATTGATG CTCATGAGGA TAGCTTCAGA TCTGCTGATG AGTCTGGGCA GGCTTTGCTT 
GCAGCTGGGC ACTATGCTTC TGATGAAGTT AAAGAAAAGC TGACTATCCT CTCAGATGAA 
AGATCAGCCT TGCTGGAACT ATGGGAGCTT CGCAGGCAAC AGTATGAGCA GTGCATGGAT 
CTGCAGCTTT TCTACAGAGA TACTGAACAA GTTGACAACT GGATGAGCAA ACAAGAAGCT 
TTTCTGCTGA ACGAAGACCT TGGTGATTCT CTGGATAGCG TGGAGGCTCT TCTAAAGAAG 
CATGAAGATT TTGAGAAATC CCTAAGTGCT CAAGAGGAGA AGATCACAGC ACTGGATGAG 
TTTGCTACTA AACTGATTCA AAATAACCAT TATGCCATGG ATGACGTTGC TACGCGCAGA 
GATGCTCTTC TGAGTCGCCG AAATGCTCTT CATGAAAGAG CCATGAAGCG CCGTGCCCAA 
CTGGCAGACT CTTTCCATCT CCAGCAGTTT TTCAGGGACT CTGATGAACT GAAGAGTTGG 
GTTAATGAAA AAATGAAAAC TGCTACCGAT GAGGCTTACA AGGATCCATC CAACTTGCAA 
GGTAAAGTTC AGAAGCATCA GGCTTTTGAA GCAGAGCTTT CTGCTAATCA GAGTCGTATT 
GATGCACTGG AGAAAGCTGG CCAGAAGTTG ATTGATGTCA ATCACTATGC ATCTGATGAG 
GTGGCAGCTC GCATGAATGA AGTCATCAGC TTGTGGAAGA AACTTCTGGA AGCCACCGAG 
CTCAAAGGTA TAAAGCTGCG TGAAGCCAAT CAACAGCAGC AATTTAATCG CAATGTAGAA 
GACATTGAGT TGTGGCTGTA TGAAGTGGAG GGTCACTTGG CTTCTGATGA TTATGGAAAA 
GATCTTACCA GCGTTCAGAA TCTTCAGAAG AAACATGCCC TGCTAGAGGC AGATGTTGCT 
GCCCATCAGG ATCGGATAGA TGGCATTACC ATCCAGGCAC GCCAGTTCCA AGATGCTGGG 
CACTTTGATG CTGACAATAT CAAGAAGAAA CAAGAAGCTT TAGTAGCTCG TTATGAAGCT 
CTGAAGGATC CTATGGTGGC TCGCAAGCAG AAACTTGCAG ATTCTCTTCG CCTGCAGCAG 
CTTTTCCGTG ACATCGAGGA TGAAGAGACC TGGATCAGGG AAAAGGAACC TATTGCAGCC 
TCAACAAACC GAGGCAAGGA CTTAATTGGT GTCCAGAATC TGCTAAAGAA GCACCAGGCT 
TTGCAGGCAG AAATTGCAGG CCATGAGCCT CGCATTAAAG CAGTCACACA AAAAGGAAAT 
GCGATGGTGG AAGAGGGACA TTTTGCTGCT GAAGATGTGA AGATCAAATT GAATGAACTA 
AACCAAAAAT GGGACTCTCT GAAAGCCAAG GCATCTCAAC GGCGACAGGA TCTAGAGGAT 
TCTCTGCAAG CTCAGCAGTA TTTTGCTGAT GCTAATGAGG CAGAATCATG GATGAGGGAA 
AAGGAGCCCA TTGTAGGCAG CACGGATTAT GGAAAGGATG AAGACTCTGC TGAGGCTCTC 
CTGAAGAAGC ATGAAGCTTT GATGTCTGAT CTTTCTGCTT ACGGCAGTAG CATACAGGCA 
TTAAGGGAAC AGGCCCAGTC CTGCAGGCAA CAAGTTGCTC CCACTGATGA TGAAACTGGA 
AAAGAGCTTG TGCTAGCACT CTATGATTAC CAAGAGAAGA GTCCTCGGGA GGTGACTATG 
AAGAAAGGAG ATATTCTAAC CCTGCTCAAC AGCACCAACA AGGACTGGTG GAAGGTTGAA 
GTTAACGATC GTCAGGGCTT TGTACCAGCT GCCTATGTGA AAAAACTAGA TCCTGCCCAG 
TCTGCATCCC GAGAGAATCT TCTAGAAGAG CAAGGCAGCA TAGCATTGCG ACAGGAGCAA 
ATTGACAACC AGTATCATTC CCTTCTTGAG CTGGGAGAAA AACGTAAAGG CATGCTAGAA 
AAAAGCTGCA AGAAGTTTAT GCTTTTCCGT GAGGCTAATG AGCTTCAACA GTGGATCAAT 
GAGAAGGAAG CTGCGCTCAC TAATGAGGAA GTGGGTGCTG ATTTGGAGCA GGTGGAGGTG 
CTACAGAAGA AGTTTGATGA TTTTCAGAAG GATTTAAAAG CTAATGAGTC ACGACTGAAG 
GACATAAACA AGGTTGCAAA TGACCTGGAG TCTGAAGGGC TGATGGCAGA GGAAGTGCAA 
GCAGTAGAGC ACCAGGAAGT CTATGGAATG ATGCCCAGAG ATGAAACTGA TTCTAAGACA 
GCCTCTCCTT GGAAGTCTGC ACGTATGATG GTACACACAG TGGCAACGTT TAACTCAATC 
AAGGAACTGA ATGAACGCTG GAGATCCCTG CAGCAGTTGG CAGAGGAGCG AAGCCAGCTG 
TTGGGCAGCG CTCATGAAGT CCAGAGGTTC CACAGAGATG CTGATGAAAC AAAAGAATGG 
ATAGAAGAGA AGAATCAAGC ATTAAATACC GACAACTATG GACATGACTT GGCCAGTGTG 
CAGGCTCTGC AGCGCAAGCA TGAAGGCTTT GAGAGAGACT TGGCAGCTCT TGGAGACAAG 
GTGAATTCTC TTGGTGAAAC TGCCCAGCGT CTGATCCAGT CACATCCAGA ATCAGCTGAA 
GATCTTCAAG AAAAATGTAC TGAGTTGAAC CAAGCCTGGA GTAGTCTGGG AAAACGTGCT 
GACCAACGCA AAGAGAAGCT TGGAGATTCT CATGACCTGC AGCGTTTCCT CAGTGATTTT 
AGAGACCTTA TGTCTTGGAT CAATGGAATC CGGGGTCTGG TCTCCTCAGA TGAACTTGCA 
AAAGATGTGA CTGGAGCTGA AGCTCTGTTG GAAAGGCATC AGGAGCACCG TACTGAAATA 
GATGCACGGG CTGGCACTTT TCAGGCATTT GAACAGTTTG GACAGCAGCT TCTGGCTCGT 
GGACACTATG CCAGCCCAGA GATTAAGGAG AAACTGGATA TTCTAGATCA AGAACGGACA 
GACCTAGAGA AGGCCTGGGT CCAGCGCAGA ATGATGCTAG ACCAGTGCTT AGAACTACAG 
CTGTTTCATC GGGATTGTGA ACAAGCTGAA AACTGGATGG CTGCCCGAGA GGCGTTCCTA 
AATACAGAAG ACAAAGGAGA CTCCTTAGAC AGCGTGGAGG CACTCATCAA GAAGCATGAA 
GATTTCGATA AAGCAATCAA TGTCCAGGAA GAGAAAATTG CTGTCTTGCA GTCTTTTGCT 
GACCAACTGA TTGCTGCTGA TCATTATGCA AAAGGAGTCA TTGCTAACAG ACGCAATGAG 
GTTCTGGACA GGTGGCGTCG TCTGAAGGCT CAGATGATTG AGAAGAGATC TAAGCTGGGA 
GAATCTCAGA CCCTCCAACA GTTCAGTCGT GATGTTGATG AAATAGAAGC TTGGATCAGT 
GAAAAGCTTC AAACTGCAAG TGATGAGTCA TATAAGGATC CCACAAACAT CCAGCTTTCC 
AAACTGCTGA GCAAGCACCA GAAGCACCAA GCCTTTGAAG CTGAGCTCCA CGCCAACGCA 
GATCGGATTC GTGGAGTCAT TGAAATGGGG AACTCTCTTA TTGAAAGAGG AGCGTGTGCT 
GGCAGCGAGG ATGCTGTGAA GGCTCGGCTG GCTGCCCTGG CTGACCAATG GGAGTTCCTG 
GTCCAGAAGT CATCAGAGAA GAGTCAGAAA CTGAAAGAAG CAAATAAACA GCAGAATTTC 
AATACCGGCA TCAAGGACTT TGATTTCTGG CTTTCAGAGG TGGAAGCTTT GTTGGCATCT 
GAAGACTATG GGAAGGACTT GGCATCAGTG AACAACCTTC TGAAGAAGCA CCAATTACTG 
GAAGCTGATA TATCTGCTCA TGAGGATCGG CTGAAGGACC TGAACAGCCA GGCTGACAGT 
TTGATGACCA GCAGTGCTTT CGATACCTCC CAAGTAAAGG ATAAACGTGA AACTATAAAT 
GGGCGCTTCC AGAGAATCAA GAGCATGGCA GCTGCCCGCC GTGCGAAGCT CAACGAGTCG 
CACCGCTTGC ATCAGTTCTT CCGTGACATG GATGATGAGG AGTCCTGGAT CAAAGAGAAG 
AAACTGTTGG TGAGCTCAGA GGACTATGGC AGAGACCTGA CTGGTGTGCA GAACCTGAGG 
AAGAAACATA AGCGCTTGGA AGCAGAATTA GCTGCCCATG AACCTGCTAT CCAGGGTGTT 
CTAGACACTG GCAAGAAGCT TTCAGATGAT AACACAATTG GGAAGGAGGA GATACAGCAA 
AGACTGGCTC AGTTTGTGGA CCACTGGAAA GAGTTAAAAC AGCTGGCAGC TGCTCGGGGG 
CAGCGTCTGG AGGAGTCCCT GGAGTACCAA CAGTTTGTAG CAAATGTTGA GGAAGAGGAG 
GCATGGATCA ACGAGAAAAT GACATTGGTA GCCAGTGAGG ATTATGGAGA CACACTTGCT 
GCTATCCAGG GCTTGCTGAA GAAGCATGAA GCATTCGAGA CTGACTTTAC TGTCCACAAA 
GACAGAGTGA ATGATGTTTG CGCTAATGGA GAGGATCTCA TTAAAAAGAA TAATCACCAT 
GTGGAGAACA TTACTGCTAA GATGAAGGGC CTCAAGGGCA AGGTATCAGA TCTGGAGAAA 
GCCGCAGCTC AGAGGAAAGC CAAACTGGAT GAGAACTCTG CCTTCCTCCA GTTCAACTGG 
AAAGCAGATG TGGTGGAGTC ATGGATAGGT GAGAAGGAAA ACAGTCTGAA GACAGATGAT 
TATGGCCGTG ACCTCTCCTC TGTGCAAACA CTACTCACCA AACAGGAAAC CTTTGATGCT 
GGACTTCAGG CTTTCCAGCA GGAGGGAATT GCAAACATCA CTGCTCTGAA AGACCAGCTA 
CTGGCAGCCA AACATATCCA ATCAAAGGCC ATTGAGGTTC GTCACGCTTC CTTGATGAAA 
CGCTGGAATC AGCTGCTTGC TAATTCTGCA GCCAGGAAAA AGAAACTCTT GGAGGCTCAG 
GAGCACTTCA GAAAGGTTGA AGATCTCTTC TTGACTTTTG CCAAGAAGGC CTCTGCCTTC 
AACAGTTGGT TTGAGAATGC TGAGGAGGAC CTGACGGATC CCGTGCGCTG CAATTCCCTG 
GAAGAAATCA AAGCACTGCG AGAAGCCCAC GATGCGTTCC GTTCCTCACT TAGCTCTGCC 
CAAGCTGACT TCAACCAGCT GGCAGAGCTT GATCGGCAGA TCAAGAGCTT CCGTGTAGCC 
TCCAACCCGT ACACTTGGTT TACTATGGAG GCTCTTGAAG AAACTTGGAG GAATCTGCAG 
AAAATTATCA AGGAGCGTGA ATTGGAGTTG CAGAAGGAGC AGCGGAGGCA GGAAGAAAAT 
GACAAGTTGC GCCAGGAGTT TGCTCAGCAT GCTAATGCCT TCCACCAGTG GATTCAGGAG 
ACAAGGACTT ACCTGCTAGA TGGGTCCTGC ATGGTGGAGG AGTCGGGAAC ACTGGAATCC 
CAGCTGGAAG CTACTAAACG CAAGCACCAG GAGATCCGGG CTATGAGGAG CCAGCTGAAG 
AAGATTGAGG ACCTTGGAGC AGCCATGGAA GAGGCACTTA TCTTGGACAA CAAATACACA 
GAGCACAGCA CCGTGGGGCT GGCGCAGCAG TGGGACCAGC TTGACCAGCT GGGAATGAGA 
ATGCAGCACA ATCTGGAACA GCAGATTCAA GCTCGAAACA CAACTGGAGT CACAGAGGAG 
GCCCTGAAGG AGTTCAGCAT GATGTTCAAG CACTTTGACA AGGACAAATC TGGACGTCTT 
AATCACCAGG AGTTTAAGTC TTGCTTGCGC TCCCTTGGCT ATGACCTGCC CATGGTTGAG 
GAAGGAGAGC CTGACCCCGA ATTTGAGTCT ATTCTTGACA CTGTTGATCC CAACAGGGAT 
GGCCACGTCT CGCTGCAGGA GTACATGGCG TTCATGATCA GCAGGGAAAC AGAGAACGTG 
AAATCCAGCG AGGAGATTGA GAGTGCTTTC CGTGCCCTCA GCTCGGAGGG GAAGCCTTAC 
GTGACCAAGG AGGAGCTCTA CCAGAACCTG ACCCGGGAGC AGGCCGACTA CTGCATTTCT 
CACATGAAGC CCTACATGGA TGGCAAGGGC AGAGAACTTC CTTCTGCCTA CGACTACATA 
GAATTTACAC GTTCACTCTT TGTGAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015134779.1
CDS	10..7419
Translation	MLSSFRKVKVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSSLRRQKLEDSYRFQFFQRDADELEKWIQEKLQIASDENYKDPSNLQGKLQKHQAFEAEVQANSGAIVKLDETGNQMINEGHFASETIRTRLQELHRLWELLLEKMREKGVKLLQAQKLVQFLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDLAAHEERVNEVNQFAGKLIQEQHPEEELIKSKQDEVNASWQRLKGLAQQRQGKLFGAAEVQRFNRDVDETISWIKEKGQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPINASQIQVKREELIANWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFRSADESGQALLAAGHYASDEVKEKLTILSDERSALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMDDVATRRDALLSRRNALHERAMKRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYASDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTSVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDADNIKKKQEALVARYEALKDPMVARKQKLADSLRLQQLFRDIEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKIKLNELNQKWDSLKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPTDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTNEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVANDLESEGLMAEEVQAVEHQEVYGMMPRDETDSKTASPWKSARMMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAQRLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKEKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLARGHYASPEIKEKLDILDQERTDLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAVLQSFADQLIAADHYAKGVIANRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIEMGNSLIERGACAGSEDAVKARLAALADQWEFLVQKSSEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRETINGRFQRIKSMAAARRAKLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVDHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCANGEDLIKKNNHHVENITAKMKGLKGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHIQSKAIEVRHASLMKRWNQLLANSAARKKKLLEAQEHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFESILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCISHMKPYMDGKGRELPSAYDYIEFTRSLFVN

Target ORF information:

RefSeq Version	XM_015279293.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279293.1

ORF Insert Sequence:

ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG 
TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC 
TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT 
GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT 
GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG 
ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAA CTCGTCTACA GGAGCTGCAC 
CGACTATGGG AGTTACTGCT GGAAAAAATG AGAGAGAAGG GAGTGAAGCT GTTGCAAGCA 
CAGAAGCTGG TGCAATTCTT ACGGGAATGT GAAGATGTCA TGGACTGGAT CAATGACAAG 
GAAGCAATAG TGACCTCAGA AGAGCTTGGA CAGGACTTAG AGCATGTTGA GGTTTTGCAG 
AAGAAATTTG AAGAGTTCCA AACAGATCTG GCAGCTCATG AGGAGAGAGT GAATGAAGTG 
AACCAGTTCG CTGGCAAACT TATCCAGGAA CAGCACCCTG AGGAGGAACT TATAAAGTCC 
AAACAGGATG AAGTAAATGC AAGCTGGCAG CGTCTTAAGG GGCTTGCCCA ACAGAGGCAA 
GGAAAACTCT TTGGGGCAGC TGAAGTTCAG CGCTTCAACA GGGATGTGGA TGAAACTATC 
AGCTGGATTA AGGAGAAAGG GCAGTTGATG GCCTCAGATG ACTTTGGCAG AGACTTGGCC 
AGCGTGCAAG CTTTACTACG TAAGCATGAA GGCCTGGAAA GAGATCTTGC AGCTTTGGAG 
GATAAGGTTA AGGCCCTCTG TGCAGAAGCT GACCGTTTGC AGCAGTCTCA CCCTATAAAT 
GCTTCTCAAA TTCAAGTGAA ACGGGAGGAA CTGATTGCCA ACTGGGAGCA GATCCGAACT 
CTGGCAGCAG AGAGGCATGC TCGCCTTAAT GACTCCTACA GGTTGCAGCG CTTTCTTGCA 
GATTTCCGGG ACCTCACTAG CTGGGTAACT GAGATGAAGG CTCTGATAAA TGCTGATGAG 
CTTGCCAATG ATGTGGCTGG AGCAGAAGCC CTTCTAGATA GACATCAGGA ACATAAGGGA 
GAAATTGATG CTCATGAGGA TAGCTTCAGA TCTGCTGATG AGTCTGGGCA GGCTTTGCTT 
GCAGCTGGGC ACTATGCTTC TGATGAAGTT AAAGAAAAGC TGACTATCCT CTCAGATGAA 
AGATCAGCCT TGCTGGAACT ATGGGAGCTT CGCAGGCAAC AGTATGAGCA GTGCATGGAT 
CTGCAGCTTT TCTACAGAGA TACTGAACAA GTTGACAACT GGATGAGCAA ACAAGAAGCT 
TTTCTGCTGA ACGAAGACCT TGGTGATTCT CTGGATAGCG TGGAGGCTCT TCTAAAGAAG 
CATGAAGATT TTGAGAAATC CCTAAGTGCT CAAGAGGAGA AGATCACAGC ACTGGATGAG 
TTTGCTACTA AACTGATTCA AAATAACCAT TATGCCATGG ATGACGTTGC TACGCGCAGA 
GATGCTCTTC TGAGTCGCCG AAATGCTCTT CATGAAAGAG CCATGAAGCG CCGTGCCCAA 
CTGGCAGACT CTTTCCATCT CCAGCAGTTT TTCAGGGACT CTGATGAACT GAAGAGTTGG 
GTTAATGAAA AAATGAAAAC TGCTACCGAT GAGGCTTACA AGGATCCATC CAACTTGCAA 
GGTAAAGTTC AGAAGCATCA GGCTTTTGAA GCAGAGCTTT CTGCTAATCA GAGTCGTATT 
GATGCACTGG AGAAAGCTGG CCAGAAGTTG ATTGATGTCA ATCACTATGC ATCTGATGAG 
GTGGCAGCTC GCATGAATGA AGTCATCAGC TTGTGGAAGA AACTTCTGGA AGCCACCGAG 
CTCAAAGGTA TAAAGCTGCG TGAAGCCAAT CAACAGCAGC AATTTAATCG CAATGTAGAA 
GACATTGAGT TGTGGCTGTA TGAAGTGGAG GGTCACTTGG CTTCTGATGA TTATGGAAAA 
GATCTTACCA GCGTTCAGAA TCTTCAGAAG AAACATGCCC TGCTAGAGGC AGATGTTGCT 
GCCCATCAGG ATCGGATAGA TGGCATTACC ATCCAGGCAC GCCAGTTCCA AGATGCTGGG 
CACTTTGATG CTGACAATAT CAAGAAGAAA CAAGAAGCTT TAGTAGCTCG TTATGAAGCT 
CTGAAGGATC CTATGGTGGC TCGCAAGCAG AAACTTGCAG ATTCTCTTCG CCTGCAGCAG 
CTTTTCCGTG ACATCGAGGA TGAAGAGACC TGGATCAGGG AAAAGGAACC TATTGCAGCC 
TCAACAAACC GAGGCAAGGA CTTAATTGGT GTCCAGAATC TGCTAAAGAA GCACCAGGCT 
TTGCAGGCAG AAATTGCAGG CCATGAGCCT CGCATTAAAG CAGTCACACA AAAAGGAAAT 
GCGATGGTGG AAGAGGGACA TTTTGCTGCT GAAGATGTGA AGATCAAATT GAATGAACTA 
AACCAAAAAT GGGACTCTCT GAAAGCCAAG GCATCTCAAC GGCGACAGGA TCTAGAGGAT 
TCTCTGCAAG CTCAGCAGTA TTTTGCTGAT GCTAATGAGG CAGAATCATG GATGAGGGAA 
AAGGAGCCCA TTGTAGGCAG CACGGATTAT GGAAAGGATG AAGACTCTGC TGAGGCTCTC 
CTGAAGAAGC ATGAAGCTTT GATGTCTGAT CTTTCTGCTT ACGGCAGTAG CATACAGGCA 
TTAAGGGAAC AGGCCCAGTC CTGCAGGCAA CAAGTTGCTC CCACTGATGA TGAAACTGGA 
AAAGAGCTTG TGCTAGCACT CTATGATTAC CAAGAGAAGA GTCCTCGGGA GGTGACTATG 
AAGAAAGGAG ATATTCTAAC CCTGCTCAAC AGCACCAACA AGGACTGGTG GAAGGTTGAA 
GTTAACGATC GTCAGGGCTT TGTACCAGCT GCCTATGTGA AAAAACTAGA TCCTGCCCAG 
TCTGCATCCC GAGAGAATCT TCTAGAAGAG CAAGGCAGCA TAGCATTGCG ACAGGAGCAA 
ATTGACAACC AGTATCATTC CCTTCTTGAG CTGGGAGAAA AACGTAAAGG CATGCTAGAA 
AAAAGCTGCA AGAAGTTTAT GCTTTTCCGT GAGGCTAATG AGCTTCAACA GTGGATCAAT 
GAGAAGGAAG CTGCGCTCAC TAATGAGGAA GTGGGTGCTG ATTTGGAGCA GGTGGAGGTG 
CTACAGAAGA AGTTTGATGA TTTTCAGAAG GATTTAAAAG CTAATGAGTC ACGACTGAAG 
GACATAAACA AGGTTGCAAA TGACCTGGAG TCTGAAGGGC TGATGGCAGA GGAAGTGCAA 
GCAGTAGAGC ACCAGGAAGT CTATGGAATG ATGCCCAGAG ATGAAACTGA TTCTAAGACA 
GCCTCTCCTT GGAAGTCTGC ACGTATGATG GTACACACAG TGGCAACGTT TAACTCAATC 
AAGGAACTGA ATGAACGCTG GAGATCCCTG CAGCAGTTGG CAGAGGAGCG AAGCCAGCTG 
TTGGGCAGCG CTCATGAAGT CCAGAGGTTC CACAGAGATG CTGATGAAAC AAAAGAATGG 
ATAGAAGAGA AGAATCAAGC ATTAAATACC GACAACTATG GACATGACTT GGCCAGTGTG 
CAGGCTCTGC AGCGCAAGCA TGAAGGCTTT GAGAGAGACT TGGCAGCTCT TGGAGACAAG 
GTGAATTCTC TTGGTGAAAC TGCCCAGCGT CTGATCCAGT CACATCCAGA ATCAGCTGAA 
GATCTTCAAG AAAAATGTAC TGAGTTGAAC CAAGCCTGGA GTAGTCTGGG AAAACGTGCT 
GACCAACGCA AAGAGAAGCT TGGAGATTCT CATGACCTGC AGCGTTTCCT CAGTGATTTT 
AGAGACCTTA TGTCTTGGAT CAATGGAATC CGGGGTCTGG TCTCCTCAGA TGAACTTGCA 
AAAGATGTGA CTGGAGCTGA AGCTCTGTTG GAAAGGCATC AGGAGCACCG TACTGAAATA 
GATGCACGGG CTGGCACTTT TCAGGCATTT GAACAGTTTG GACAGCAGCT TCTGGCTCGT 
GGACACTATG CCAGCCCAGA GATTAAGGAG AAACTGGATA TTCTAGATCA AGAACGGACA 
GACCTAGAGA AGGCCTGGGT CCAGCGCAGA ATGATGCTAG ACCAGTGCTT AGAACTACAG 
CTGTTTCATC GGGATTGTGA ACAAGCTGAA AACTGGATGG CTGCCCGAGA GGCGTTCCTA 
AATACAGAAG ACAAAGGAGA CTCCTTAGAC AGCGTGGAGG CACTCATCAA GAAGCATGAA 
GATTTCGATA AAGCAATCAA TGTCCAGGAA GAGAAAATTG CTGTCTTGCA GTCTTTTGCT 
GACCAACTGA TTGCTGCTGA TCATTATGCA AAAGGAGTCA TTGCTAACAG ACGCAATGAG 
GTTCTGGACA GGTGGCGTCG TCTGAAGGCT CAGATGATTG AGAAGAGATC TAAGCTGGGA 
GAATCTCAGA CCCTCCAACA GTTCAGTCGT GATGTTGATG AAATAGAAGC TTGGATCAGT 
GAAAAGCTTC AAACTGCAAG TGATGAGTCA TATAAGGATC CCACAAACAT CCAGCTTTCC 
AAACTGCTGA GCAAGCACCA GAAGCACCAA GCCTTTGAAG CTGAGCTCCA CGCCAACGCA 
GATCGGATTC GTGGAGTCAT TGAAATGGGG AACTCTCTTA TTGAAAGAGG AGCGTGTGCT 
GGCAGCGAGG ATGCTGTGAA GGCTCGGCTG GCTGCCCTGG CTGACCAATG GGAGTTCCTG 
GTCCAGAAGT CATCAGAGAA GAGTCAGAAA CTGAAAGAAG CAAATAAACA GCAGAATTTC 
AATACCGGCA TCAAGGACTT TGATTTCTGG CTTTCAGAGG TGGAAGCTTT GTTGGCATCT 
GAAGACTATG GGAAGGACTT GGCATCAGTG AACAACCTTC TGAAGAAGCA CCAATTACTG 
GAAGCTGATA TATCTGCTCA TGAGGATCGG CTGAAGGACC TGAACAGCCA GGCTGACAGT 
TTGATGACCA GCAGTGCTTT CGATACCTCC CAAGTAAAGG ATAAACGTGA AACTATAAAT 
GGGCGCTTCC AGAGAATCAA GAGCATGGCA GCTGCCCGCC GTGCGAAGCT CAACGAGTCG 
CACCGCTTGC ATCAGTTCTT CCGTGACATG GATGATGAGG AGTCCTGGAT CAAAGAGAAG 
AAACTGTTGG TGAGCTCAGA GGACTATGGC AGAGACCTGA CTGGTGTGCA GAACCTGAGG 
AAGAAACATA AGCGCTTGGA AGCAGAATTA GCTGCCCATG AACCTGCTAT CCAGGGTGTT 
CTAGACACTG GCAAGAAGCT TTCAGATGAT AACACAATTG GGAAGGAGGA GATACAGCAA 
AGACTGGCTC AGTTTGTGGA CCACTGGAAA GAGTTAAAAC AGCTGGCAGC TGCTCGGGGG 
CAGCGTCTGG AGGAGTCCCT GGAGTACCAA CAGTTTGTAG CAAATGTTGA GGAAGAGGAG 
GCATGGATCA ACGAGAAAAT GACATTGGTA GCCAGTGAGG ATTATGGAGA CACACTTGCT 
GCTATCCAGG GCTTGCTGAA GAAGCATGAA GCATTCGAGA CTGACTTTAC TGTCCACAAA 
GACAGAGTGA ATGATGTTTG CGCTAATGGA GAGGATCTCA TTAAAAAGAA TAATCACCAT 
GTGGAGAACA TTACTGCTAA GATGAAGGGC CTCAAGGGCA AGGTATCAGA TCTGGAGAAA 
GCCGCAGCTC AGAGGAAAGC CAAACTGGAT GAGAACTCTG CCTTCCTCCA GTTCAACTGG 
AAAGCAGATG TGGTGGAGTC ATGGATAGGT GAGAAGGAAA ACAGTCTGAA GACAGATGAT 
TATGGCCGTG ACCTCTCCTC TGTGCAAACA CTACTCACCA AACAGGAAAC CTTTGATGCT 
GGACTTCAGG CTTTCCAGCA GGAGGGAATT GCAAACATCA CTGCTCTGAA AGACCAGCTA 
CTGGCAGCCA AACATATCCA ATCAAAGGCC ATTGAGGTTC GTCACGCTTC CTTGATGAAA 
CGCTGGAATC AGCTGCTTGC TAATTCTGCA GCCAGGAAAA AGAAACTCTT GGAGGCTCAG 
GAGCACTTCA GAAAGGTTGA AGATCTCTTC TTGACTTTTG CCAAGAAGGC CTCTGCCTTC 
AACAGTTGGT TTGAGAATGC TGAGGAGGAC CTGACGGATC CCGTGCGCTG CAATTCCCTG 
GAAGAAATCA AAGCACTGCG AGAAGCCCAC GATGCGTTCC GTTCCTCACT TAGCTCTGCC 
CAAGCTGACT TCAACCAGCT GGCAGAGCTT GATCGGCAGA TCAAGAGCTT CCGTGTAGCC 
TCCAACCCGT ACACTTGGTT TACTATGGAG GCTCTTGAAG AAACTTGGAG GAATCTGCAG 
AAAATTATCA AGGAGCGTGA ATTGGAGTTG CAGAAGGAGC AGCGGAGGCA GGAAGAAAAT 
GACAAGTTGC GCCAGGAGTT TGCTCAGCAT GCTAATGCCT TCCACCAGTG GATTCAGGAG 
ACAAGGACTT ACCTGCTAGA TGGGTCCTGC ATGGTGGAGG AGTCGGGAAC ACTGGAATCC 
CAGCTGGAAG CTACTAAACG CAAGCACCAG GAGATCCGGG CTATGAGGAG CCAGCTGAAG 
AAGATTGAGG ACCTTGGAGC AGCCATGGAA GAGGCACTTA TCTTGGACAA CAAATACACA 
GAGCACAGCA CCGTGGGGCT GGCGCAGCAG TGGGACCAGC TTGACCAGCT GGGAATGAGA 
ATGCAGCACA ATCTGGAACA GCAGATTCAA GCTCGAAACA CAACTGGAGT CACAGAGGAG 
GCCCTGAAGG AGTTCAGCAT GATGTTCAAG CACTTTGACA AGGACAAATC TGGACGTCTT 
AATCACCAGG AGTTTAAGTC TTGCTTGCGC TCCCTTGGCT ATGACCTGCC CATGGTTGAG 
GAAGGAGAGC CTGACCCCGA ATTTGAGTCT ATTCTTGACA CTGTTGATCC CAACAGGGAT 
GGCCACGTCT CGCTGCAGGA GTACATGGCG TTCATGATCA GCAGGGAAAC AGAGAACGTG 
AAATCCAGCG AGGAGATTGA GAGTGCTTTC CGTGCCCTCA GCTCGGAGGG GAAGCCTTAC 
GTGACCAAGG AGGAGCTCTA CCAGAACCTG ACCCGGGAGC AGGCCGACTA CTGCATTTCT 
CACATGAAGC CCTACATGGA TGGCAAGGGC AGAGAACTTC CTTCTGCCTA CGACTACATA 
GAATTTACAC GTTCACTCTT TGTGAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_015279293.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015134779.1 Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X12, mRNA.

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CloneID	OGa43319
Clone ID Related Accession (Same CDS sequence)	XM_015279293.1 , XM_015279293.2
Accession Version	XM_015279293.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7410bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015279293.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG 
TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC 
TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT 
GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT 
GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG 
ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAA CTCGTCTACA GGAGCTGCAC 
CGACTATGGG AGTTACTGCT GGAAAAAATG AGAGAGAAGG GAGTGAAGCT GTTGCAAGCA 
CAGAAGCTGG TGCAATTCTT ACGGGAATGT GAAGATGTCA TGGACTGGAT CAATGACAAG 
GAAGCAATAG TGACCTCAGA AGAGCTTGGA CAGGACTTAG AGCATGTTGA GGTTTTGCAG 
AAGAAATTTG AAGAGTTCCA AACAGATCTG GCAGCTCATG AGGAGAGAGT GAATGAAGTG 
AACCAGTTCG CTGGCAAACT TATCCAGGAA CAGCACCCTG AGGAGGAACT TATAAAGTCC 
AAACAGGATG AAGTAAATGC AAGCTGGCAG CGTCTTAAGG GGCTTGCCCA ACAGAGGCAA 
GGAAAACTCT TTGGGGCAGC TGAAGTTCAG CGCTTCAACA GGGATGTGGA TGAAACTATC 
AGCTGGATTA AGGAGAAAGG GCAGTTGATG GCCTCAGATG ACTTTGGCAG AGACTTGGCC 
AGCGTGCAAG CTTTACTACG TAAGCATGAA GGCCTGGAAA GAGATCTTGC AGCTTTGGAG 
GATAAGGTTA AGGCCCTCTG TGCAGAAGCT GACCGTTTGC AGCAGTCTCA CCCTATAAAT 
GCTTCTCAAA TTCAAGTGAA ACGGGAGGAA CTGATTGCCA ACTGGGAGCA GATCCGAACT 
CTGGCAGCAG AGAGGCATGC TCGCCTTAAT GACTCCTACA GGTTGCAGCG CTTTCTTGCA 
GATTTCCGGG ACCTCACTAG CTGGGTAACT GAGATGAAGG CTCTGATAAA TGCTGATGAG 
CTTGCCAATG ATGTGGCTGG AGCAGAAGCC CTTCTAGATA GACATCAGGA ACATAAGGGA 
GAAATTGATG CTCATGAGGA TAGCTTCAGA TCTGCTGATG AGTCTGGGCA GGCTTTGCTT 
GCAGCTGGGC ACTATGCTTC TGATGAAGTT AAAGAAAAGC TGACTATCCT CTCAGATGAA 
AGATCAGCCT TGCTGGAACT ATGGGAGCTT CGCAGGCAAC AGTATGAGCA GTGCATGGAT 
CTGCAGCTTT TCTACAGAGA TACTGAACAA GTTGACAACT GGATGAGCAA ACAAGAAGCT 
TTTCTGCTGA ACGAAGACCT TGGTGATTCT CTGGATAGCG TGGAGGCTCT TCTAAAGAAG 
CATGAAGATT TTGAGAAATC CCTAAGTGCT CAAGAGGAGA AGATCACAGC ACTGGATGAG 
TTTGCTACTA AACTGATTCA AAATAACCAT TATGCCATGG ATGACGTTGC TACGCGCAGA 
GATGCTCTTC TGAGTCGCCG AAATGCTCTT CATGAAAGAG CCATGAAGCG CCGTGCCCAA 
CTGGCAGACT CTTTCCATCT CCAGCAGTTT TTCAGGGACT CTGATGAACT GAAGAGTTGG 
GTTAATGAAA AAATGAAAAC TGCTACCGAT GAGGCTTACA AGGATCCATC CAACTTGCAA 
GGTAAAGTTC AGAAGCATCA GGCTTTTGAA GCAGAGCTTT CTGCTAATCA GAGTCGTATT 
GATGCACTGG AGAAAGCTGG CCAGAAGTTG ATTGATGTCA ATCACTATGC ATCTGATGAG 
GTGGCAGCTC GCATGAATGA AGTCATCAGC TTGTGGAAGA AACTTCTGGA AGCCACCGAG 
CTCAAAGGTA TAAAGCTGCG TGAAGCCAAT CAACAGCAGC AATTTAATCG CAATGTAGAA 
GACATTGAGT TGTGGCTGTA TGAAGTGGAG GGTCACTTGG CTTCTGATGA TTATGGAAAA 
GATCTTACCA GCGTTCAGAA TCTTCAGAAG AAACATGCCC TGCTAGAGGC AGATGTTGCT 
GCCCATCAGG ATCGGATAGA TGGCATTACC ATCCAGGCAC GCCAGTTCCA AGATGCTGGG 
CACTTTGATG CTGACAATAT CAAGAAGAAA CAAGAAGCTT TAGTAGCTCG TTATGAAGCT 
CTGAAGGATC CTATGGTGGC TCGCAAGCAG AAACTTGCAG ATTCTCTTCG CCTGCAGCAG 
CTTTTCCGTG ACATCGAGGA TGAAGAGACC TGGATCAGGG AAAAGGAACC TATTGCAGCC 
TCAACAAACC GAGGCAAGGA CTTAATTGGT GTCCAGAATC TGCTAAAGAA GCACCAGGCT 
TTGCAGGCAG AAATTGCAGG CCATGAGCCT CGCATTAAAG CAGTCACACA AAAAGGAAAT 
GCGATGGTGG AAGAGGGACA TTTTGCTGCT GAAGATGTGA AGATCAAATT GAATGAACTA 
AACCAAAAAT GGGACTCTCT GAAAGCCAAG GCATCTCAAC GGCGACAGGA TCTAGAGGAT 
TCTCTGCAAG CTCAGCAGTA TTTTGCTGAT GCTAATGAGG CAGAATCATG GATGAGGGAA 
AAGGAGCCCA TTGTAGGCAG CACGGATTAT GGAAAGGATG AAGACTCTGC TGAGGCTCTC 
CTGAAGAAGC ATGAAGCTTT GATGTCTGAT CTTTCTGCTT ACGGCAGTAG CATACAGGCA 
TTAAGGGAAC AGGCCCAGTC CTGCAGGCAA CAAGTTGCTC CCACTGATGA TGAAACTGGA 
AAAGAGCTTG TGCTAGCACT CTATGATTAC CAAGAGAAGA GTCCTCGGGA GGTGACTATG 
AAGAAAGGAG ATATTCTAAC CCTGCTCAAC AGCACCAACA AGGACTGGTG GAAGGTTGAA 
GTTAACGATC GTCAGGGCTT TGTACCAGCT GCCTATGTGA AAAAACTAGA TCCTGCCCAG 
TCTGCATCCC GAGAGAATCT TCTAGAAGAG CAAGGCAGCA TAGCATTGCG ACAGGAGCAA 
ATTGACAACC AGTATCATTC CCTTCTTGAG CTGGGAGAAA AACGTAAAGG CATGCTAGAA 
AAAAGCTGCA AGAAGTTTAT GCTTTTCCGT GAGGCTAATG AGCTTCAACA GTGGATCAAT 
GAGAAGGAAG CTGCGCTCAC TAATGAGGAA GTGGGTGCTG ATTTGGAGCA GGTGGAGGTG 
CTACAGAAGA AGTTTGATGA TTTTCAGAAG GATTTAAAAG CTAATGAGTC ACGACTGAAG 
GACATAAACA AGGTTGCAAA TGACCTGGAG TCTGAAGGGC TGATGGCAGA GGAAGTGCAA 
GCAGTAGAGC ACCAGGAAGT CTATGGAATG ATGCCCAGAG ATGAAACTGA TTCTAAGACA 
GCCTCTCCTT GGAAGTCTGC ACGTATGATG GTACACACAG TGGCAACGTT TAACTCAATC 
AAGGAACTGA ATGAACGCTG GAGATCCCTG CAGCAGTTGG CAGAGGAGCG AAGCCAGCTG 
TTGGGCAGCG CTCATGAAGT CCAGAGGTTC CACAGAGATG CTGATGAAAC AAAAGAATGG 
ATAGAAGAGA AGAATCAAGC ATTAAATACC GACAACTATG GACATGACTT GGCCAGTGTG 
CAGGCTCTGC AGCGCAAGCA TGAAGGCTTT GAGAGAGACT TGGCAGCTCT TGGAGACAAG 
GTGAATTCTC TTGGTGAAAC TGCCCAGCGT CTGATCCAGT CACATCCAGA ATCAGCTGAA 
GATCTTCAAG AAAAATGTAC TGAGTTGAAC CAAGCCTGGA GTAGTCTGGG AAAACGTGCT 
GACCAACGCA AAGAGAAGCT TGGAGATTCT CATGACCTGC AGCGTTTCCT CAGTGATTTT 
AGAGACCTTA TGTCTTGGAT CAATGGAATC CGGGGTCTGG TCTCCTCAGA TGAACTTGCA 
AAAGATGTGA CTGGAGCTGA AGCTCTGTTG GAAAGGCATC AGGAGCACCG TACTGAAATA 
GATGCACGGG CTGGCACTTT TCAGGCATTT GAACAGTTTG GACAGCAGCT TCTGGCTCGT 
GGACACTATG CCAGCCCAGA GATTAAGGAG AAACTGGATA TTCTAGATCA AGAACGGACA 
GACCTAGAGA AGGCCTGGGT CCAGCGCAGA ATGATGCTAG ACCAGTGCTT AGAACTACAG 
CTGTTTCATC GGGATTGTGA ACAAGCTGAA AACTGGATGG CTGCCCGAGA GGCGTTCCTA 
AATACAGAAG ACAAAGGAGA CTCCTTAGAC AGCGTGGAGG CACTCATCAA GAAGCATGAA 
GATTTCGATA AAGCAATCAA TGTCCAGGAA GAGAAAATTG CTGTCTTGCA GTCTTTTGCT 
GACCAACTGA TTGCTGCTGA TCATTATGCA AAAGGAGTCA TTGCTAACAG ACGCAATGAG 
GTTCTGGACA GGTGGCGTCG TCTGAAGGCT CAGATGATTG AGAAGAGATC TAAGCTGGGA 
GAATCTCAGA CCCTCCAACA GTTCAGTCGT GATGTTGATG AAATAGAAGC TTGGATCAGT 
GAAAAGCTTC AAACTGCAAG TGATGAGTCA TATAAGGATC CCACAAACAT CCAGCTTTCC 
AAACTGCTGA GCAAGCACCA GAAGCACCAA GCCTTTGAAG CTGAGCTCCA CGCCAACGCA 
GATCGGATTC GTGGAGTCAT TGAAATGGGG AACTCTCTTA TTGAAAGAGG AGCGTGTGCT 
GGCAGCGAGG ATGCTGTGAA GGCTCGGCTG GCTGCCCTGG CTGACCAATG GGAGTTCCTG 
GTCCAGAAGT CATCAGAGAA GAGTCAGAAA CTGAAAGAAG CAAATAAACA GCAGAATTTC 
AATACCGGCA TCAAGGACTT TGATTTCTGG CTTTCAGAGG TGGAAGCTTT GTTGGCATCT 
GAAGACTATG GGAAGGACTT GGCATCAGTG AACAACCTTC TGAAGAAGCA CCAATTACTG 
GAAGCTGATA TATCTGCTCA TGAGGATCGG CTGAAGGACC TGAACAGCCA GGCTGACAGT 
TTGATGACCA GCAGTGCTTT CGATACCTCC CAAGTAAAGG ATAAACGTGA AACTATAAAT 
GGGCGCTTCC AGAGAATCAA GAGCATGGCA GCTGCCCGCC GTGCGAAGCT CAACGAGTCG 
CACCGCTTGC ATCAGTTCTT CCGTGACATG GATGATGAGG AGTCCTGGAT CAAAGAGAAG 
AAACTGTTGG TGAGCTCAGA GGACTATGGC AGAGACCTGA CTGGTGTGCA GAACCTGAGG 
AAGAAACATA AGCGCTTGGA AGCAGAATTA GCTGCCCATG AACCTGCTAT CCAGGGTGTT 
CTAGACACTG GCAAGAAGCT TTCAGATGAT AACACAATTG GGAAGGAGGA GATACAGCAA 
AGACTGGCTC AGTTTGTGGA CCACTGGAAA GAGTTAAAAC AGCTGGCAGC TGCTCGGGGG 
CAGCGTCTGG AGGAGTCCCT GGAGTACCAA CAGTTTGTAG CAAATGTTGA GGAAGAGGAG 
GCATGGATCA ACGAGAAAAT GACATTGGTA GCCAGTGAGG ATTATGGAGA CACACTTGCT 
GCTATCCAGG GCTTGCTGAA GAAGCATGAA GCATTCGAGA CTGACTTTAC TGTCCACAAA 
GACAGAGTGA ATGATGTTTG CGCTAATGGA GAGGATCTCA TTAAAAAGAA TAATCACCAT 
GTGGAGAACA TTACTGCTAA GATGAAGGGC CTCAAGGGCA AGGTATCAGA TCTGGAGAAA 
GCCGCAGCTC AGAGGAAAGC CAAACTGGAT GAGAACTCTG CCTTCCTCCA GTTCAACTGG 
AAAGCAGATG TGGTGGAGTC ATGGATAGGT GAGAAGGAAA ACAGTCTGAA GACAGATGAT 
TATGGCCGTG ACCTCTCCTC TGTGCAAACA CTACTCACCA AACAGGAAAC CTTTGATGCT 
GGACTTCAGG CTTTCCAGCA GGAGGGAATT GCAAACATCA CTGCTCTGAA AGACCAGCTA 
CTGGCAGCCA AACATATCCA ATCAAAGGCC ATTGAGGTTC GTCACGCTTC CTTGATGAAA 
CGCTGGAATC AGCTGCTTGC TAATTCTGCA GCCAGGAAAA AGAAACTCTT GGAGGCTCAG 
GAGCACTTCA GAAAGGTTGA AGATCTCTTC TTGACTTTTG CCAAGAAGGC CTCTGCCTTC 
AACAGTTGGT TTGAGAATGC TGAGGAGGAC CTGACGGATC CCGTGCGCTG CAATTCCCTG 
GAAGAAATCA AAGCACTGCG AGAAGCCCAC GATGCGTTCC GTTCCTCACT TAGCTCTGCC 
CAAGCTGACT TCAACCAGCT GGCAGAGCTT GATCGGCAGA TCAAGAGCTT CCGTGTAGCC 
TCCAACCCGT ACACTTGGTT TACTATGGAG GCTCTTGAAG AAACTTGGAG GAATCTGCAG 
AAAATTATCA AGGAGCGTGA ATTGGAGTTG CAGAAGGAGC AGCGGAGGCA GGAAGAAAAT 
GACAAGTTGC GCCAGGAGTT TGCTCAGCAT GCTAATGCCT TCCACCAGTG GATTCAGGAG 
ACAAGGACTT ACCTGCTAGA TGGGTCCTGC ATGGTGGAGG AGTCGGGAAC ACTGGAATCC 
CAGCTGGAAG CTACTAAACG CAAGCACCAG GAGATCCGGG CTATGAGGAG CCAGCTGAAG 
AAGATTGAGG ACCTTGGAGC AGCCATGGAA GAGGCACTTA TCTTGGACAA CAAATACACA 
GAGCACAGCA CCGTGGGGCT GGCGCAGCAG TGGGACCAGC TTGACCAGCT GGGAATGAGA 
ATGCAGCACA ATCTGGAACA GCAGATTCAA GCTCGAAACA CAACTGGAGT CACAGAGGAG 
GCCCTGAAGG AGTTCAGCAT GATGTTCAAG CACTTTGACA AGGACAAATC TGGACGTCTT 
AATCACCAGG AGTTTAAGTC TTGCTTGCGC TCCCTTGGCT ATGACCTGCC CATGGTTGAG 
GAAGGAGAGC CTGACCCCGA ATTTGAGTCT ATTCTTGACA CTGTTGATCC CAACAGGGAT 
GGCCACGTCT CGCTGCAGGA GTACATGGCG TTCATGATCA GCAGGGAAAC AGAGAACGTG 
AAATCCAGCG AGGAGATTGA GAGTGCTTTC CGTGCCCTCA GCTCGGAGGG GAAGCCTTAC 
GTGACCAAGG AGGAGCTCTA CCAGAACCTG ACCCGGGAGC AGGCCGACTA CTGCATTTCT 
CACATGAAGC CCTACATGGA TGGCAAGGGC AGAGAACTTC CTTCTGCCTA CGACTACATA 
GAATTTACAC GTTCACTCTT TGTGAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015134779.1
CDS	7..7416
Translation	MLSSFRKVKVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSSLRRQKLEDSYRFQFFQRDADELEKWIQEKLQIASDENYKDPSNLQGKLQKHQAFEAEVQANSGAIVKLDETGNQMINEGHFASETIRTRLQELHRLWELLLEKMREKGVKLLQAQKLVQFLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDLAAHEERVNEVNQFAGKLIQEQHPEEELIKSKQDEVNASWQRLKGLAQQRQGKLFGAAEVQRFNRDVDETISWIKEKGQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPINASQIQVKREELIANWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFRSADESGQALLAAGHYASDEVKEKLTILSDERSALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMDDVATRRDALLSRRNALHERAMKRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYASDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTSVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDADNIKKKQEALVARYEALKDPMVARKQKLADSLRLQQLFRDIEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKIKLNELNQKWDSLKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPTDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTNEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVANDLESEGLMAEEVQAVEHQEVYGMMPRDETDSKTASPWKSARMMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAQRLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKEKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLARGHYASPEIKEKLDILDQERTDLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAVLQSFADQLIAADHYAKGVIANRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIEMGNSLIERGACAGSEDAVKARLAALADQWEFLVQKSSEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRETINGRFQRIKSMAAARRAKLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVDHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCANGEDLIKKNNHHVENITAKMKGLKGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHIQSKAIEVRHASLMKRWNQLLANSAARKKKLLEAQEHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFESILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCISHMKPYMDGKGRELPSAYDYIEFTRSLFVN

Target ORF information:

RefSeq Version	XM_015279293.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279293.2

ORF Insert Sequence:

ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG 
TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC 
TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT 
GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT 
GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG 
ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAA CTCGTCTACA GGAGCTGCAC 
CGACTATGGG AGTTACTGCT GGAAAAAATG AGAGAGAAGG GAGTGAAGCT GTTGCAAGCA 
CAGAAGCTGG TGCAATTCTT ACGGGAATGT GAAGATGTCA TGGACTGGAT CAATGACAAG 
GAAGCAATAG TGACCTCAGA AGAGCTTGGA CAGGACTTAG AGCATGTTGA GGTTTTGCAG 
AAGAAATTTG AAGAGTTCCA AACAGATCTG GCAGCTCATG AGGAGAGAGT GAATGAAGTG 
AACCAGTTCG CTGGCAAACT TATCCAGGAA CAGCACCCTG AGGAGGAACT TATAAAGTCC 
AAACAGGATG AAGTAAATGC AAGCTGGCAG CGTCTTAAGG GGCTTGCCCA ACAGAGGCAA 
GGAAAACTCT TTGGGGCAGC TGAAGTTCAG CGCTTCAACA GGGATGTGGA TGAAACTATC 
AGCTGGATTA AGGAGAAAGG GCAGTTGATG GCCTCAGATG ACTTTGGCAG AGACTTGGCC 
AGCGTGCAAG CTTTACTACG TAAGCATGAA GGCCTGGAAA GAGATCTTGC AGCTTTGGAG 
GATAAGGTTA AGGCCCTCTG TGCAGAAGCT GACCGTTTGC AGCAGTCTCA CCCTATAAAT 
GCTTCTCAAA TTCAAGTGAA ACGGGAGGAA CTGATTGCCA ACTGGGAGCA GATCCGAACT 
CTGGCAGCAG AGAGGCATGC TCGCCTTAAT GACTCCTACA GGTTGCAGCG CTTTCTTGCA 
GATTTCCGGG ACCTCACTAG CTGGGTAACT GAGATGAAGG CTCTGATAAA TGCTGATGAG 
CTTGCCAATG ATGTGGCTGG AGCAGAAGCC CTTCTAGATA GACATCAGGA ACATAAGGGA 
GAAATTGATG CTCATGAGGA TAGCTTCAGA TCTGCTGATG AGTCTGGGCA GGCTTTGCTT 
GCAGCTGGGC ACTATGCTTC TGATGAAGTT AAAGAAAAGC TGACTATCCT CTCAGATGAA 
AGATCAGCCT TGCTGGAACT ATGGGAGCTT CGCAGGCAAC AGTATGAGCA GTGCATGGAT 
CTGCAGCTTT TCTACAGAGA TACTGAACAA GTTGACAACT GGATGAGCAA ACAAGAAGCT 
TTTCTGCTGA ACGAAGACCT TGGTGATTCT CTGGATAGCG TGGAGGCTCT TCTAAAGAAG 
CATGAAGATT TTGAGAAATC CCTAAGTGCT CAAGAGGAGA AGATCACAGC ACTGGATGAG 
TTTGCTACTA AACTGATTCA AAATAACCAT TATGCCATGG ATGACGTTGC TACGCGCAGA 
GATGCTCTTC TGAGTCGCCG AAATGCTCTT CATGAAAGAG CCATGAAGCG CCGTGCCCAA 
CTGGCAGACT CTTTCCATCT CCAGCAGTTT TTCAGGGACT CTGATGAACT GAAGAGTTGG 
GTTAATGAAA AAATGAAAAC TGCTACCGAT GAGGCTTACA AGGATCCATC CAACTTGCAA 
GGTAAAGTTC AGAAGCATCA GGCTTTTGAA GCAGAGCTTT CTGCTAATCA GAGTCGTATT 
GATGCACTGG AGAAAGCTGG CCAGAAGTTG ATTGATGTCA ATCACTATGC ATCTGATGAG 
GTGGCAGCTC GCATGAATGA AGTCATCAGC TTGTGGAAGA AACTTCTGGA AGCCACCGAG 
CTCAAAGGTA TAAAGCTGCG TGAAGCCAAT CAACAGCAGC AATTTAATCG CAATGTAGAA 
GACATTGAGT TGTGGCTGTA TGAAGTGGAG GGTCACTTGG CTTCTGATGA TTATGGAAAA 
GATCTTACCA GCGTTCAGAA TCTTCAGAAG AAACATGCCC TGCTAGAGGC AGATGTTGCT 
GCCCATCAGG ATCGGATAGA TGGCATTACC ATCCAGGCAC GCCAGTTCCA AGATGCTGGG 
CACTTTGATG CTGACAATAT CAAGAAGAAA CAAGAAGCTT TAGTAGCTCG TTATGAAGCT 
CTGAAGGATC CTATGGTGGC TCGCAAGCAG AAACTTGCAG ATTCTCTTCG CCTGCAGCAG 
CTTTTCCGTG ACATCGAGGA TGAAGAGACC TGGATCAGGG AAAAGGAACC TATTGCAGCC 
TCAACAAACC GAGGCAAGGA CTTAATTGGT GTCCAGAATC TGCTAAAGAA GCACCAGGCT 
TTGCAGGCAG AAATTGCAGG CCATGAGCCT CGCATTAAAG CAGTCACACA AAAAGGAAAT 
GCGATGGTGG AAGAGGGACA TTTTGCTGCT GAAGATGTGA AGATCAAATT GAATGAACTA 
AACCAAAAAT GGGACTCTCT GAAAGCCAAG GCATCTCAAC GGCGACAGGA TCTAGAGGAT 
TCTCTGCAAG CTCAGCAGTA TTTTGCTGAT GCTAATGAGG CAGAATCATG GATGAGGGAA 
AAGGAGCCCA TTGTAGGCAG CACGGATTAT GGAAAGGATG AAGACTCTGC TGAGGCTCTC 
CTGAAGAAGC ATGAAGCTTT GATGTCTGAT CTTTCTGCTT ACGGCAGTAG CATACAGGCA 
TTAAGGGAAC AGGCCCAGTC CTGCAGGCAA CAAGTTGCTC CCACTGATGA TGAAACTGGA 
AAAGAGCTTG TGCTAGCACT CTATGATTAC CAAGAGAAGA GTCCTCGGGA GGTGACTATG 
AAGAAAGGAG ATATTCTAAC CCTGCTCAAC AGCACCAACA AGGACTGGTG GAAGGTTGAA 
GTTAACGATC GTCAGGGCTT TGTACCAGCT GCCTATGTGA AAAAACTAGA TCCTGCCCAG 
TCTGCATCCC GAGAGAATCT TCTAGAAGAG CAAGGCAGCA TAGCATTGCG ACAGGAGCAA 
ATTGACAACC AGTATCATTC CCTTCTTGAG CTGGGAGAAA AACGTAAAGG CATGCTAGAA 
AAAAGCTGCA AGAAGTTTAT GCTTTTCCGT GAGGCTAATG AGCTTCAACA GTGGATCAAT 
GAGAAGGAAG CTGCGCTCAC TAATGAGGAA GTGGGTGCTG ATTTGGAGCA GGTGGAGGTG 
CTACAGAAGA AGTTTGATGA TTTTCAGAAG GATTTAAAAG CTAATGAGTC ACGACTGAAG 
GACATAAACA AGGTTGCAAA TGACCTGGAG TCTGAAGGGC TGATGGCAGA GGAAGTGCAA 
GCAGTAGAGC ACCAGGAAGT CTATGGAATG ATGCCCAGAG ATGAAACTGA TTCTAAGACA 
GCCTCTCCTT GGAAGTCTGC ACGTATGATG GTACACACAG TGGCAACGTT TAACTCAATC 
AAGGAACTGA ATGAACGCTG GAGATCCCTG CAGCAGTTGG CAGAGGAGCG AAGCCAGCTG 
TTGGGCAGCG CTCATGAAGT CCAGAGGTTC CACAGAGATG CTGATGAAAC AAAAGAATGG 
ATAGAAGAGA AGAATCAAGC ATTAAATACC GACAACTATG GACATGACTT GGCCAGTGTG 
CAGGCTCTGC AGCGCAAGCA TGAAGGCTTT GAGAGAGACT TGGCAGCTCT TGGAGACAAG 
GTGAATTCTC TTGGTGAAAC TGCCCAGCGT CTGATCCAGT CACATCCAGA ATCAGCTGAA 
GATCTTCAAG AAAAATGTAC TGAGTTGAAC CAAGCCTGGA GTAGTCTGGG AAAACGTGCT 
GACCAACGCA AAGAGAAGCT TGGAGATTCT CATGACCTGC AGCGTTTCCT CAGTGATTTT 
AGAGACCTTA TGTCTTGGAT CAATGGAATC CGGGGTCTGG TCTCCTCAGA TGAACTTGCA 
AAAGATGTGA CTGGAGCTGA AGCTCTGTTG GAAAGGCATC AGGAGCACCG TACTGAAATA 
GATGCACGGG CTGGCACTTT TCAGGCATTT GAACAGTTTG GACAGCAGCT TCTGGCTCGT 
GGACACTATG CCAGCCCAGA GATTAAGGAG AAACTGGATA TTCTAGATCA AGAACGGACA 
GACCTAGAGA AGGCCTGGGT CCAGCGCAGA ATGATGCTAG ACCAGTGCTT AGAACTACAG 
CTGTTTCATC GGGATTGTGA ACAAGCTGAA AACTGGATGG CTGCCCGAGA GGCGTTCCTA 
AATACAGAAG ACAAAGGAGA CTCCTTAGAC AGCGTGGAGG CACTCATCAA GAAGCATGAA 
GATTTCGATA AAGCAATCAA TGTCCAGGAA GAGAAAATTG CTGTCTTGCA GTCTTTTGCT 
GACCAACTGA TTGCTGCTGA TCATTATGCA AAAGGAGTCA TTGCTAACAG ACGCAATGAG 
GTTCTGGACA GGTGGCGTCG TCTGAAGGCT CAGATGATTG AGAAGAGATC TAAGCTGGGA 
GAATCTCAGA CCCTCCAACA GTTCAGTCGT GATGTTGATG AAATAGAAGC TTGGATCAGT 
GAAAAGCTTC AAACTGCAAG TGATGAGTCA TATAAGGATC CCACAAACAT CCAGCTTTCC 
AAACTGCTGA GCAAGCACCA GAAGCACCAA GCCTTTGAAG CTGAGCTCCA CGCCAACGCA 
GATCGGATTC GTGGAGTCAT TGAAATGGGG AACTCTCTTA TTGAAAGAGG AGCGTGTGCT 
GGCAGCGAGG ATGCTGTGAA GGCTCGGCTG GCTGCCCTGG CTGACCAATG GGAGTTCCTG 
GTCCAGAAGT CATCAGAGAA GAGTCAGAAA CTGAAAGAAG CAAATAAACA GCAGAATTTC 
AATACCGGCA TCAAGGACTT TGATTTCTGG CTTTCAGAGG TGGAAGCTTT GTTGGCATCT 
GAAGACTATG GGAAGGACTT GGCATCAGTG AACAACCTTC TGAAGAAGCA CCAATTACTG 
GAAGCTGATA TATCTGCTCA TGAGGATCGG CTGAAGGACC TGAACAGCCA GGCTGACAGT 
TTGATGACCA GCAGTGCTTT CGATACCTCC CAAGTAAAGG ATAAACGTGA AACTATAAAT 
GGGCGCTTCC AGAGAATCAA GAGCATGGCA GCTGCCCGCC GTGCGAAGCT CAACGAGTCG 
CACCGCTTGC ATCAGTTCTT CCGTGACATG GATGATGAGG AGTCCTGGAT CAAAGAGAAG 
AAACTGTTGG TGAGCTCAGA GGACTATGGC AGAGACCTGA CTGGTGTGCA GAACCTGAGG 
AAGAAACATA AGCGCTTGGA AGCAGAATTA GCTGCCCATG AACCTGCTAT CCAGGGTGTT 
CTAGACACTG GCAAGAAGCT TTCAGATGAT AACACAATTG GGAAGGAGGA GATACAGCAA 
AGACTGGCTC AGTTTGTGGA CCACTGGAAA GAGTTAAAAC AGCTGGCAGC TGCTCGGGGG 
CAGCGTCTGG AGGAGTCCCT GGAGTACCAA CAGTTTGTAG CAAATGTTGA GGAAGAGGAG 
GCATGGATCA ACGAGAAAAT GACATTGGTA GCCAGTGAGG ATTATGGAGA CACACTTGCT 
GCTATCCAGG GCTTGCTGAA GAAGCATGAA GCATTCGAGA CTGACTTTAC TGTCCACAAA 
GACAGAGTGA ATGATGTTTG CGCTAATGGA GAGGATCTCA TTAAAAAGAA TAATCACCAT 
GTGGAGAACA TTACTGCTAA GATGAAGGGC CTCAAGGGCA AGGTATCAGA TCTGGAGAAA 
GCCGCAGCTC AGAGGAAAGC CAAACTGGAT GAGAACTCTG CCTTCCTCCA GTTCAACTGG 
AAAGCAGATG TGGTGGAGTC ATGGATAGGT GAGAAGGAAA ACAGTCTGAA GACAGATGAT 
TATGGCCGTG ACCTCTCCTC TGTGCAAACA CTACTCACCA AACAGGAAAC CTTTGATGCT 
GGACTTCAGG CTTTCCAGCA GGAGGGAATT GCAAACATCA CTGCTCTGAA AGACCAGCTA 
CTGGCAGCCA AACATATCCA ATCAAAGGCC ATTGAGGTTC GTCACGCTTC CTTGATGAAA 
CGCTGGAATC AGCTGCTTGC TAATTCTGCA GCCAGGAAAA AGAAACTCTT GGAGGCTCAG 
GAGCACTTCA GAAAGGTTGA AGATCTCTTC TTGACTTTTG CCAAGAAGGC CTCTGCCTTC 
AACAGTTGGT TTGAGAATGC TGAGGAGGAC CTGACGGATC CCGTGCGCTG CAATTCCCTG 
GAAGAAATCA AAGCACTGCG AGAAGCCCAC GATGCGTTCC GTTCCTCACT TAGCTCTGCC 
CAAGCTGACT TCAACCAGCT GGCAGAGCTT GATCGGCAGA TCAAGAGCTT CCGTGTAGCC 
TCCAACCCGT ACACTTGGTT TACTATGGAG GCTCTTGAAG AAACTTGGAG GAATCTGCAG 
AAAATTATCA AGGAGCGTGA ATTGGAGTTG CAGAAGGAGC AGCGGAGGCA GGAAGAAAAT 
GACAAGTTGC GCCAGGAGTT TGCTCAGCAT GCTAATGCCT TCCACCAGTG GATTCAGGAG 
ACAAGGACTT ACCTGCTAGA TGGGTCCTGC ATGGTGGAGG AGTCGGGAAC ACTGGAATCC 
CAGCTGGAAG CTACTAAACG CAAGCACCAG GAGATCCGGG CTATGAGGAG CCAGCTGAAG 
AAGATTGAGG ACCTTGGAGC AGCCATGGAA GAGGCACTTA TCTTGGACAA CAAATACACA 
GAGCACAGCA CCGTGGGGCT GGCGCAGCAG TGGGACCAGC TTGACCAGCT GGGAATGAGA 
ATGCAGCACA ATCTGGAACA GCAGATTCAA GCTCGAAACA CAACTGGAGT CACAGAGGAG 
GCCCTGAAGG AGTTCAGCAT GATGTTCAAG CACTTTGACA AGGACAAATC TGGACGTCTT 
AATCACCAGG AGTTTAAGTC TTGCTTGCGC TCCCTTGGCT ATGACCTGCC CATGGTTGAG 
GAAGGAGAGC CTGACCCCGA ATTTGAGTCT ATTCTTGACA CTGTTGATCC CAACAGGGAT 
GGCCACGTCT CGCTGCAGGA GTACATGGCG TTCATGATCA GCAGGGAAAC AGAGAACGTG 
AAATCCAGCG AGGAGATTGA GAGTGCTTTC CGTGCCCTCA GCTCGGAGGG GAAGCCTTAC 
GTGACCAAGG AGGAGCTCTA CCAGAACCTG ACCCGGGAGC AGGCCGACTA CTGCATTTCT 
CACATGAAGC CCTACATGGA TGGCAAGGGC AGAGAACTTC CTTCTGCCTA CGACTACATA 
GAATTTACAC GTTCACTCTT TGTGAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_015279294.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015134780.1 Gallus gallus spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X13, mRNA.

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CloneID	OGa43320
Clone ID Related Accession (Same CDS sequence)	XM_015279294.1 , XM_015279294.2
Accession Version	XM_015279294.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG 
TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC 
TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT 
GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT 
GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG 
ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAA CTCGTCTACA GGAGCTGCAC 
CGACTATGGG AGTTACTGCT GGAAAAAATG AGAGAGAAGG GAGTGAAGCT GTTGCAAGCA 
CAGAAGCTGG TGCAATTCTT ACGGGAATGT GAAGATGTCA TGGACTGGAT CAATGACAAG 
GAAGCAATAG TGACCTCAGA AGAGCTTGGA CAGGACTTAG AGCATGTTGA GGTTTTGCAG 
AAGAAATTTG AAGAGTTCCA AACAGATCTG GCAGCTCATG AGGAGAGAGT GAATGAAGTG 
AACCAGTTCG CTGGCAAACT TATCCAGGAA CAGCACCCTG AGGAGGAACT TATAAAGTCC 
AAACAGGATG AAGTAAATGC AAGCTGGCAG CGTCTTAAGG GGCTTGCCCA ACAGAGGCAA 
GGAAAACTCT TTGGGGCAGC TGAAGTTCAG CGCTTCAACA GGGATGTGGA TGAAACTATC 
AGCTGGATTA AGGAGAAAGG GCAGTTGATG GCCTCAGATG ACTTTGGCAG AGACTTGGCC 
AGCGTGCAAG CTTTACTACG TAAGCATGAA GGCCTGGAAA GAGATCTTGC AGCTTTGGAG 
GATAAGGTTA AGGCCCTCTG TGCAGAAGCT GACCGTTTGC AGCAGTCTCA CCCTATAAAT 
GCTTCTCAAA TTCAAGTGAA ACGGGAGGAA CTGATTGCCA ACTGGGAGCA GATCCGAACT 
CTGGCAGCAG AGAGGCATGC TCGCCTTAAT GACTCCTACA GGTTGCAGCG CTTTCTTGCA 
GATTTCCGGG ACCTCACTAG CTGGGTAACT GAGATGAAGG CTCTGATAAA TGCTGATGAG 
CTTGCCAATG ATGTGGCTGG AGCAGAAGCC CTTCTAGATA GACATCAGGA ACATAAGGGA 
GAAATTGATG CTCATGAGGA TAGCTTCAGA TCTGCTGATG AGTCTGGGCA GGCTTTGCTT 
GCAGCTGGGC ACTATGCTTC TGATGAAGTT AAAGAAAAGC TGACTATCCT CTCAGATGAA 
AGATCAGCCT TGCTGGAACT ATGGGAGCTT CGCAGGCAAC AGTATGAGCA GTGCATGGAT 
CTGCAGCTTT TCTACAGAGA TACTGAACAA GTTGACAACT GGATGAGCAA ACAAGAAGCT 
TTTCTGCTGA ACGAAGACCT TGGTGATTCT CTGGATAGCG TGGAGGCTCT TCTAAAGAAG 
CATGAAGATT TTGAGAAATC CCTAAGTGCT CAAGAGGAGA AGATCACAGC ACTGGATGAG 
TTTGCTACTA AACTGATTCA AAATAACCAT TATGCCATGG ATGACGTTGC TACGCGCAGA 
GATGCTCTTC TGAGTCGCCG AAATGCTCTT CATGAAAGAG CCATGAAGCG CCGTGCCCAA 
CTGGCAGACT CTTTCCATCT CCAGCAGTTT TTCAGGGACT CTGATGAACT GAAGAGTTGG 
GTTAATGAAA AAATGAAAAC TGCTACCGAT GAGGCTTACA AGGATCCATC CAACTTGCAA 
GGTAAAGTTC AGAAGCATCA GGCTTTTGAA GCAGAGCTTT CTGCTAATCA GAGTCGTATT 
GATGCACTGG AGAAAGCTGG CCAGAAGTTG ATTGATGTCA ATCACTATGC ATCTGATGAG 
GTGGCAGCTC GCATGAATGA AGTCATCAGC TTGTGGAAGA AACTTCTGGA AGCCACCGAG 
CTCAAAGGTA TAAAGCTGCG TGAAGCCAAT CAACAGCAGC AATTTAATCG CAATGTAGAA 
GACATTGAGT TGTGGCTGTA TGAAGTGGAG GGTCACTTGG CTTCTGATGA TTATGGAAAA 
GATCTTACCA GCGTTCAGAA TCTTCAGAAG AAACATGCCC TGCTAGAGGC AGATGTTGCT 
GCCCATCAGG ATCGGATAGA TGGCATTACC ATCCAGGCAC GCCAGTTCCA AGATGCTGGG 
CACTTTGATG CTGACAATAT CAAGAAGAAA CAAGAAGCTT TAGTAGCTCG TTATGAAGCT 
CTGAAGGATC CTATGGTGGC TCGCAAGCAG AAACTTGCAG ATTCTCTTCG CCTGCAGCAG 
CTTTTCCGTG ACATCGAGGA TGAAGAGACC TGGATCAGGG AAAAGGAACC TATTGCAGCC 
TCAACAAACC GAGGCAAGGA CTTAATTGGT GTCCAGAATC TGCTAAAGAA GCACCAGGCT 
TTGCAGGCAG AAATTGCAGG CCATGAGCCT CGCATTAAAG CAGTCACACA AAAAGGAAAT 
GCGATGGTGG AAGAGGGACA TTTTGCTGCT GAAGATGTGA AGATCAAATT GAATGAACTA 
AACCAAAAAT GGGACTCTCT GAAAGCCAAG GCATCTCAAC GGCGACAGGA TCTAGAGGAT 
TCTCTGCAAG CTCAGCAGTA TTTTGCTGAT GCTAATGAGG CAGAATCATG GATGAGGGAA 
AAGGAGCCCA TTGTAGGCAG CACGGATTAT GGAAAGGATG AAGACTCTGC TGAGGCTCTC 
CTGAAGAAGC ATGAAGCTTT GATGTCTGAT CTTTCTGCTT ACGGCAGTAG CATACAGGCA 
TTAAGGGAAC AGGCCCAGTC CTGCAGGCAA CAAGTTGCTC CCACTGATGA TGAAACTGGA 
AAAGAGCTTG TGCTAGCACT CTATGATTAC CAAGAGAAGA GTCCTCGGGA GGTGACTATG 
AAGAAAGGAG ATATTCTAAC CCTGCTCAAC AGCACCAACA AGGACTGGTG GAAGGTTGAA 
GTTAACGATC GTCAGGGCTT TGTACCAGCT GCCTATGTGA AAAAACTAGA TCCTGCCCAG 
TCTGCATCCC GAGAGAATCT TCTAGAAGAG CAAGGCAGCA TAGCATTGCG ACAGGAGCAA 
ATTGACAACC AGTATCATTC CCTTCTTGAG CTGGGAGAAA AACGTAAAGG CATGCTAGAA 
AAAAGCTGCA AGAAGTTTAT GCTTTTCCGT GAGGCTAATG AGCTTCAACA GTGGATCAAT 
GAGAAGGAAG CTGCGCTCAC TAATGAGGAA GTGGGTGCTG ATTTGGAGCA GGTGGAGGTG 
CTACAGAAGA AGTTTGATGA TTTTCAGAAG GATTTAAAAG CTAATGAGTC ACGACTGAAG 
GACATAAACA AGGTTGCAAA TGACCTGGAG TCTGAAGGGC TGATGGCAGA GGAAGTGCAA 
GCAGTAGAGC ACCAGGAAGT CTATGGAATG ATGCCCAGAG ATGAAACTGA TTCTAAGACA 
GCCTCTCCTT GGAAGTCTGC ACGTATGATG GTACACACAG TGGCAACGTT TAACTCAATC 
AAGGAACTGA ATGAACGCTG GAGATCCCTG CAGCAGTTGG CAGAGGAGCG AAGCCAGCTG 
TTGGGCAGCG CTCATGAAGT CCAGAGGTTC CACAGAGATG CTGATGAAAC AAAAGAATGG 
ATAGAAGAGA AGAATCAAGC ATTAAATACC GACAACTATG GACATGACTT GGCCAGTGTG 
CAGGCTCTGC AGCGCAAGCA TGAAGGCTTT GAGAGAGACT TGGCAGCTCT TGGAGACAAG 
GTGAATTCTC TTGGTGAAAC TGCCCAGCGT CTGATCCAGT CACATCCAGA ATCAGCTGAA 
GATCTTCAAG AAAAATGTAC TGAGTTGAAC CAAGCCTGGA GTAGTCTGGG AAAACGTGCT 
GACCAACGCA AAGAGAAGCT TGGAGATTCT CATGACCTGC AGCGTTTCCT CAGTGATTTT 
AGAGACCTTA TGTCTTGGAT CAATGGAATC CGGGGTCTGG TCTCCTCAGA TGAACTTGCA 
AAAGATGTGA CTGGAGCTGA AGCTCTGTTG GAAAGGCATC AGGAGCACCG TACTGAAATA 
GATGCACGGG CTGGCACTTT TCAGGCATTT GAACAGTTTG GACAGCAGCT TCTGGCTCGT 
GGACACTATG CCAGCCCAGA GATTAAGGAG AAACTGGATA TTCTAGATCA AGAACGGACA 
GACCTAGAGA AGGCCTGGGT CCAGCGCAGA ATGATGCTAG ACCAGTGCTT AGAACTACAG 
CTGTTTCATC GGGATTGTGA ACAAGCTGAA AACTGGATGG CTGCCCGAGA GGCGTTCCTA 
AATACAGAAG ACAAAGGAGA CTCCTTAGAC AGCGTGGAGG CACTCATCAA GAAGCATGAA 
GATTTCGATA AAGCAATCAA TGTCCAGGAA GAGAAAATTG CTGTCTTGCA GTCTTTTGCT 
GACCAACTGA TTGCTGCTGA TCATTATGCA AAAGGAGTCA TTGCTAACAG ACGCAATGAG 
GTTCTGGACA GGTGGCGTCG TCTGAAGGCT CAGATGATTG AGAAGAGATC TAAGCTGGGA 
GAATCTCAGA CCCTCCAACA GTTCAGTCGT GATGTTGATG AAATAGAAGC TTGGATCAGT 
GAAAAGCTTC AAACTGCAAG TGATGAGTCA TATAAGGATC CCACAAACAT CCAGAGCAAG 
CACCAGAAGC ACCAAGCCTT TGAAGCTGAG CTCCACGCCA ACGCAGATCG GATTCGTGGA 
GTCATTGAAA TGGGGAACTC TCTTATTGAA AGAGGAGCGT GTGCTGGCAG CGAGGATGCT 
GTGAAGGCTC GGCTGGCTGC CCTGGCTGAC CAATGGGAGT TCCTGGTCCA GAAGTCATCA 
GAGAAGAGTC AGAAACTGAA AGAAGCAAAT AAACAGCAGA ATTTCAATAC CGGCATCAAG 
GACTTTGATT TCTGGCTTTC AGAGGTGGAA GCTTTGTTGG CATCTGAAGA CTATGGGAAG 
GACTTGGCAT CAGTGAACAA CCTTCTGAAG AAGCACCAAT TACTGGAAGC TGATATATCT 
GCTCATGAGG ATCGGCTGAA GGACCTGAAC AGCCAGGCTG ACAGTTTGAT GACCAGCAGT 
GCTTTCGATA CCTCCCAAGT AAAGGATAAA CGTGAAACTA TAAATGGGCG CTTCCAGAGA 
ATCAAGAGCA TGGCAGCTGC CCGCCGTGCG AAGCTCAACG AGTCGCACCG CTTGCATCAG 
TTCTTCCGTG ACATGGATGA TGAGGAGTCC TGGATCAAAG AGAAGAAACT GTTGGTGAGC 
TCAGAGGACT ATGGCAGAGA CCTGACTGGT GTGCAGAACC TGAGGAAGAA ACATAAGCGC 
TTGGAAGCAG AATTAGCTGC CCATGAACCT GCTATCCAGG GTGTTCTAGA CACTGGCAAG 
AAGCTTTCAG ATGATAACAC AATTGGGAAG GAGGAGATAC AGCAAAGACT GGCTCAGTTT 
GTGGACCACT GGAAAGAGTT AAAACAGCTG GCAGCTGCTC GGGGGCAGCG TCTGGAGGAG 
TCCCTGGAGT ACCAACAGTT TGTAGCAAAT GTTGAGGAAG AGGAGGCATG GATCAACGAG 
AAAATGACAT TGGTAGCCAG TGAGGATTAT GGAGACACAC TTGCTGCTAT CCAGGGCTTG 
CTGAAGAAGC ATGAAGCATT CGAGACTGAC TTTACTGTCC ACAAAGACAG AGTGAATGAT 
GTTTGCGCTA ATGGAGAGGA TCTCATTAAA AAGAATAATC ACCATGTGGA GAACATTACT 
GCTAAGATGA AGGGCCTCAA GGGCAAGGTA TCAGATCTGG AGAAAGCCGC AGCTCAGAGG 
AAAGCCAAAC TGGATGAGAA CTCTGCCTTC CTCCAGTTCA ACTGGAAAGC AGATGTGGTG 
GAGTCATGGA TAGGTGAGAA GGAAAACAGT CTGAAGACAG ATGATTATGG CCGTGACCTC 
TCCTCTGTGC AAACACTACT CACCAAACAG GAAACCTTTG ATGCTGGACT TCAGGCTTTC 
CAGCAGGAGG GAATTGCAAA CATCACTGCT CTGAAAGACC AGCTACTGGC AGCCAAACAT 
ATCCAATCAA AGGCCATTGA GGTTCGTCAC GCTTCCTTGA TGAAACGCTG GAATCAGCTG 
CTTGCTAATT CTGCAGCCAG GAAAAAGAAA CTCTTGGAGG CTCAGGAGCA CTTCAGAAAG 
GTTGAAGATC TCTTCTTGAC TTTTGCCAAG AAGGCCTCTG CCTTCAACAG TTGGTTTGAG 
AATGCTGAGG AGGACCTGAC GGATCCCGTG CGCTGCAATT CCCTGGAAGA AATCAAAGCA 
CTGCGAGAAG CCCACGATGC GTTCCGTTCC TCACTTAGCT CTGCCCAAGC TGACTTCAAC 
CAGCTGGCAG AGCTTGATCG GCAGATCAAG AGCTTCCGTG TAGCCTCCAA CCCGTACACT 
TGGTTTACTA TGGAGGCTCT TGAAGAAACT TGGAGGAATC TGCAGAAAAT TATCAAGGAG 
CGTGAATTGG AGTTGCAGAA GGAGCAGCGG AGGCAGGAAG AAAATGACAA GTTGCGCCAG 
GAGTTTGCTC AGCATGCTAA TGCCTTCCAC CAGTGGATTC AGGAGACAAG GACTTACCTG 
CTAGATGGGT CCTGCATGGT GGAGGAGTCG GGAACACTGG AATCCCAGCT GGAAGCTACT 
AAACGCAAGC ACCAGGAGAT CCGGGCTATG AGGAGCCAGC TGAAGAAGAT TGAGGACCTT 
GGAGCAGCCA TGGAAGAGGC ACTTATCTTG GACAACAAAT ACACAGAGCA CAGCACCGTG 
GGGCTGGCGC AGCAGTGGGA CCAGCTTGAC CAGCTGGGAA TGAGAATGCA GCACAATCTG 
GAACAGCAGA TTCAAGCTCG AAACACAACT GGAGTCACAG AGGAGGCCCT GAAGGAGTTC 
AGCATGATGT TCAAGCACTT TGACAAGGAC AAATCTGGAC GTCTTAATCA CCAGGAGTTT 
AAGTCTTGCT TGCGCTCCCT TGGCTATGAC CTGCCCATGG TTGAGGAAGG AGAGCCTGAC 
CCCGAATTTG AGTCTATTCT TGACACTGTT GATCCCAACA GGGATGGCCA CGTCTCGCTG 
CAGGAGTACA TGGCGTTCAT GATCAGCAGG GAAACAGAGA ACGTGAAATC CAGCGAGGAG 
ATTGAGAGTG CTTTCCGTGC CCTCAGCTCG GAGGGGAAGC CTTACGTGAC CAAGGAGGAG 
CTCTACCAGA ACCTGACCCG GGAGCAGGCC GACTACTGCA TTTCTCACAT GAAGCCCTAC 
ATGGATGGCA AGGGCAGAGA ACTTCCTTCT GCCTACGACT ACATAGAATT TACACGTTCA 
CTCTTTGTGA ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015134780.1
CDS	9..7403
Translation	MLSSFRKVKVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSSLRRQKLEDSYRFQFFQRDADELEKWIQEKLQIASDENYKDPSNLQGKLQKHQAFEAEVQANSGAIVKLDETGNQMINEGHFASETIRTRLQELHRLWELLLEKMREKGVKLLQAQKLVQFLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDLAAHEERVNEVNQFAGKLIQEQHPEEELIKSKQDEVNASWQRLKGLAQQRQGKLFGAAEVQRFNRDVDETISWIKEKGQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPINASQIQVKREELIANWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFRSADESGQALLAAGHYASDEVKEKLTILSDERSALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMDDVATRRDALLSRRNALHERAMKRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYASDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTSVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDADNIKKKQEALVARYEALKDPMVARKQKLADSLRLQQLFRDIEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKIKLNELNQKWDSLKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPTDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTNEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVANDLESEGLMAEEVQAVEHQEVYGMMPRDETDSKTASPWKSARMMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAQRLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKEKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLARGHYASPEIKEKLDILDQERTDLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAVLQSFADQLIAADHYAKGVIANRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQSKHQKHQAFEAELHANADRIRGVIEMGNSLIERGACAGSEDAVKARLAALADQWEFLVQKSSEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRETINGRFQRIKSMAAARRAKLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVDHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCANGEDLIKKNNHHVENITAKMKGLKGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHIQSKAIEVRHASLMKRWNQLLANSAARKKKLLEAQEHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFESILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCISHMKPYMDGKGRELPSAYDYIEFTRSLFVN

Target ORF information:

RefSeq Version	XM_015279294.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279294.1

ORF Insert Sequence:

ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG 
TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC 
TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT 
GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT 
GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG 
ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAA CTCGTCTACA GGAGCTGCAC 
CGACTATGGG AGTTACTGCT GGAAAAAATG AGAGAGAAGG GAGTGAAGCT GTTGCAAGCA 
CAGAAGCTGG TGCAATTCTT ACGGGAATGT GAAGATGTCA TGGACTGGAT CAATGACAAG 
GAAGCAATAG TGACCTCAGA AGAGCTTGGA CAGGACTTAG AGCATGTTGA GGTTTTGCAG 
AAGAAATTTG AAGAGTTCCA AACAGATCTG GCAGCTCATG AGGAGAGAGT GAATGAAGTG 
AACCAGTTCG CTGGCAAACT TATCCAGGAA CAGCACCCTG AGGAGGAACT TATAAAGTCC 
AAACAGGATG AAGTAAATGC AAGCTGGCAG CGTCTTAAGG GGCTTGCCCA ACAGAGGCAA 
GGAAAACTCT TTGGGGCAGC TGAAGTTCAG CGCTTCAACA GGGATGTGGA TGAAACTATC 
AGCTGGATTA AGGAGAAAGG GCAGTTGATG GCCTCAGATG ACTTTGGCAG AGACTTGGCC 
AGCGTGCAAG CTTTACTACG TAAGCATGAA GGCCTGGAAA GAGATCTTGC AGCTTTGGAG 
GATAAGGTTA AGGCCCTCTG TGCAGAAGCT GACCGTTTGC AGCAGTCTCA CCCTATAAAT 
GCTTCTCAAA TTCAAGTGAA ACGGGAGGAA CTGATTGCCA ACTGGGAGCA GATCCGAACT 
CTGGCAGCAG AGAGGCATGC TCGCCTTAAT GACTCCTACA GGTTGCAGCG CTTTCTTGCA 
GATTTCCGGG ACCTCACTAG CTGGGTAACT GAGATGAAGG CTCTGATAAA TGCTGATGAG 
CTTGCCAATG ATGTGGCTGG AGCAGAAGCC CTTCTAGATA GACATCAGGA ACATAAGGGA 
GAAATTGATG CTCATGAGGA TAGCTTCAGA TCTGCTGATG AGTCTGGGCA GGCTTTGCTT 
GCAGCTGGGC ACTATGCTTC TGATGAAGTT AAAGAAAAGC TGACTATCCT CTCAGATGAA 
AGATCAGCCT TGCTGGAACT ATGGGAGCTT CGCAGGCAAC AGTATGAGCA GTGCATGGAT 
CTGCAGCTTT TCTACAGAGA TACTGAACAA GTTGACAACT GGATGAGCAA ACAAGAAGCT 
TTTCTGCTGA ACGAAGACCT TGGTGATTCT CTGGATAGCG TGGAGGCTCT TCTAAAGAAG 
CATGAAGATT TTGAGAAATC CCTAAGTGCT CAAGAGGAGA AGATCACAGC ACTGGATGAG 
TTTGCTACTA AACTGATTCA AAATAACCAT TATGCCATGG ATGACGTTGC TACGCGCAGA 
GATGCTCTTC TGAGTCGCCG AAATGCTCTT CATGAAAGAG CCATGAAGCG CCGTGCCCAA 
CTGGCAGACT CTTTCCATCT CCAGCAGTTT TTCAGGGACT CTGATGAACT GAAGAGTTGG 
GTTAATGAAA AAATGAAAAC TGCTACCGAT GAGGCTTACA AGGATCCATC CAACTTGCAA 
GGTAAAGTTC AGAAGCATCA GGCTTTTGAA GCAGAGCTTT CTGCTAATCA GAGTCGTATT 
GATGCACTGG AGAAAGCTGG CCAGAAGTTG ATTGATGTCA ATCACTATGC ATCTGATGAG 
GTGGCAGCTC GCATGAATGA AGTCATCAGC TTGTGGAAGA AACTTCTGGA AGCCACCGAG 
CTCAAAGGTA TAAAGCTGCG TGAAGCCAAT CAACAGCAGC AATTTAATCG CAATGTAGAA 
GACATTGAGT TGTGGCTGTA TGAAGTGGAG GGTCACTTGG CTTCTGATGA TTATGGAAAA 
GATCTTACCA GCGTTCAGAA TCTTCAGAAG AAACATGCCC TGCTAGAGGC AGATGTTGCT 
GCCCATCAGG ATCGGATAGA TGGCATTACC ATCCAGGCAC GCCAGTTCCA AGATGCTGGG 
CACTTTGATG CTGACAATAT CAAGAAGAAA CAAGAAGCTT TAGTAGCTCG TTATGAAGCT 
CTGAAGGATC CTATGGTGGC TCGCAAGCAG AAACTTGCAG ATTCTCTTCG CCTGCAGCAG 
CTTTTCCGTG ACATCGAGGA TGAAGAGACC TGGATCAGGG AAAAGGAACC TATTGCAGCC 
TCAACAAACC GAGGCAAGGA CTTAATTGGT GTCCAGAATC TGCTAAAGAA GCACCAGGCT 
TTGCAGGCAG AAATTGCAGG CCATGAGCCT CGCATTAAAG CAGTCACACA AAAAGGAAAT 
GCGATGGTGG AAGAGGGACA TTTTGCTGCT GAAGATGTGA AGATCAAATT GAATGAACTA 
AACCAAAAAT GGGACTCTCT GAAAGCCAAG GCATCTCAAC GGCGACAGGA TCTAGAGGAT 
TCTCTGCAAG CTCAGCAGTA TTTTGCTGAT GCTAATGAGG CAGAATCATG GATGAGGGAA 
AAGGAGCCCA TTGTAGGCAG CACGGATTAT GGAAAGGATG AAGACTCTGC TGAGGCTCTC 
CTGAAGAAGC ATGAAGCTTT GATGTCTGAT CTTTCTGCTT ACGGCAGTAG CATACAGGCA 
TTAAGGGAAC AGGCCCAGTC CTGCAGGCAA CAAGTTGCTC CCACTGATGA TGAAACTGGA 
AAAGAGCTTG TGCTAGCACT CTATGATTAC CAAGAGAAGA GTCCTCGGGA GGTGACTATG 
AAGAAAGGAG ATATTCTAAC CCTGCTCAAC AGCACCAACA AGGACTGGTG GAAGGTTGAA 
GTTAACGATC GTCAGGGCTT TGTACCAGCT GCCTATGTGA AAAAACTAGA TCCTGCCCAG 
TCTGCATCCC GAGAGAATCT TCTAGAAGAG CAAGGCAGCA TAGCATTGCG ACAGGAGCAA 
ATTGACAACC AGTATCATTC CCTTCTTGAG CTGGGAGAAA AACGTAAAGG CATGCTAGAA 
AAAAGCTGCA AGAAGTTTAT GCTTTTCCGT GAGGCTAATG AGCTTCAACA GTGGATCAAT 
GAGAAGGAAG CTGCGCTCAC TAATGAGGAA GTGGGTGCTG ATTTGGAGCA GGTGGAGGTG 
CTACAGAAGA AGTTTGATGA TTTTCAGAAG GATTTAAAAG CTAATGAGTC ACGACTGAAG 
GACATAAACA AGGTTGCAAA TGACCTGGAG TCTGAAGGGC TGATGGCAGA GGAAGTGCAA 
GCAGTAGAGC ACCAGGAAGT CTATGGAATG ATGCCCAGAG ATGAAACTGA TTCTAAGACA 
GCCTCTCCTT GGAAGTCTGC ACGTATGATG GTACACACAG TGGCAACGTT TAACTCAATC 
AAGGAACTGA ATGAACGCTG GAGATCCCTG CAGCAGTTGG CAGAGGAGCG AAGCCAGCTG 
TTGGGCAGCG CTCATGAAGT CCAGAGGTTC CACAGAGATG CTGATGAAAC AAAAGAATGG 
ATAGAAGAGA AGAATCAAGC ATTAAATACC GACAACTATG GACATGACTT GGCCAGTGTG 
CAGGCTCTGC AGCGCAAGCA TGAAGGCTTT GAGAGAGACT TGGCAGCTCT TGGAGACAAG 
GTGAATTCTC TTGGTGAAAC TGCCCAGCGT CTGATCCAGT CACATCCAGA ATCAGCTGAA 
GATCTTCAAG AAAAATGTAC TGAGTTGAAC CAAGCCTGGA GTAGTCTGGG AAAACGTGCT 
GACCAACGCA AAGAGAAGCT TGGAGATTCT CATGACCTGC AGCGTTTCCT CAGTGATTTT 
AGAGACCTTA TGTCTTGGAT CAATGGAATC CGGGGTCTGG TCTCCTCAGA TGAACTTGCA 
AAAGATGTGA CTGGAGCTGA AGCTCTGTTG GAAAGGCATC AGGAGCACCG TACTGAAATA 
GATGCACGGG CTGGCACTTT TCAGGCATTT GAACAGTTTG GACAGCAGCT TCTGGCTCGT 
GGACACTATG CCAGCCCAGA GATTAAGGAG AAACTGGATA TTCTAGATCA AGAACGGACA 
GACCTAGAGA AGGCCTGGGT CCAGCGCAGA ATGATGCTAG ACCAGTGCTT AGAACTACAG 
CTGTTTCATC GGGATTGTGA ACAAGCTGAA AACTGGATGG CTGCCCGAGA GGCGTTCCTA 
AATACAGAAG ACAAAGGAGA CTCCTTAGAC AGCGTGGAGG CACTCATCAA GAAGCATGAA 
GATTTCGATA AAGCAATCAA TGTCCAGGAA GAGAAAATTG CTGTCTTGCA GTCTTTTGCT 
GACCAACTGA TTGCTGCTGA TCATTATGCA AAAGGAGTCA TTGCTAACAG ACGCAATGAG 
GTTCTGGACA GGTGGCGTCG TCTGAAGGCT CAGATGATTG AGAAGAGATC TAAGCTGGGA 
GAATCTCAGA CCCTCCAACA GTTCAGTCGT GATGTTGATG AAATAGAAGC TTGGATCAGT 
GAAAAGCTTC AAACTGCAAG TGATGAGTCA TATAAGGATC CCACAAACAT CCAGAGCAAG 
CACCAGAAGC ACCAAGCCTT TGAAGCTGAG CTCCACGCCA ACGCAGATCG GATTCGTGGA 
GTCATTGAAA TGGGGAACTC TCTTATTGAA AGAGGAGCGT GTGCTGGCAG CGAGGATGCT 
GTGAAGGCTC GGCTGGCTGC CCTGGCTGAC CAATGGGAGT TCCTGGTCCA GAAGTCATCA 
GAGAAGAGTC AGAAACTGAA AGAAGCAAAT AAACAGCAGA ATTTCAATAC CGGCATCAAG 
GACTTTGATT TCTGGCTTTC AGAGGTGGAA GCTTTGTTGG CATCTGAAGA CTATGGGAAG 
GACTTGGCAT CAGTGAACAA CCTTCTGAAG AAGCACCAAT TACTGGAAGC TGATATATCT 
GCTCATGAGG ATCGGCTGAA GGACCTGAAC AGCCAGGCTG ACAGTTTGAT GACCAGCAGT 
GCTTTCGATA CCTCCCAAGT AAAGGATAAA CGTGAAACTA TAAATGGGCG CTTCCAGAGA 
ATCAAGAGCA TGGCAGCTGC CCGCCGTGCG AAGCTCAACG AGTCGCACCG CTTGCATCAG 
TTCTTCCGTG ACATGGATGA TGAGGAGTCC TGGATCAAAG AGAAGAAACT GTTGGTGAGC 
TCAGAGGACT ATGGCAGAGA CCTGACTGGT GTGCAGAACC TGAGGAAGAA ACATAAGCGC 
TTGGAAGCAG AATTAGCTGC CCATGAACCT GCTATCCAGG GTGTTCTAGA CACTGGCAAG 
AAGCTTTCAG ATGATAACAC AATTGGGAAG GAGGAGATAC AGCAAAGACT GGCTCAGTTT 
GTGGACCACT GGAAAGAGTT AAAACAGCTG GCAGCTGCTC GGGGGCAGCG TCTGGAGGAG 
TCCCTGGAGT ACCAACAGTT TGTAGCAAAT GTTGAGGAAG AGGAGGCATG GATCAACGAG 
AAAATGACAT TGGTAGCCAG TGAGGATTAT GGAGACACAC TTGCTGCTAT CCAGGGCTTG 
CTGAAGAAGC ATGAAGCATT CGAGACTGAC TTTACTGTCC ACAAAGACAG AGTGAATGAT 
GTTTGCGCTA ATGGAGAGGA TCTCATTAAA AAGAATAATC ACCATGTGGA GAACATTACT 
GCTAAGATGA AGGGCCTCAA GGGCAAGGTA TCAGATCTGG AGAAAGCCGC AGCTCAGAGG 
AAAGCCAAAC TGGATGAGAA CTCTGCCTTC CTCCAGTTCA ACTGGAAAGC AGATGTGGTG 
GAGTCATGGA TAGGTGAGAA GGAAAACAGT CTGAAGACAG ATGATTATGG CCGTGACCTC 
TCCTCTGTGC AAACACTACT CACCAAACAG GAAACCTTTG ATGCTGGACT TCAGGCTTTC 
CAGCAGGAGG GAATTGCAAA CATCACTGCT CTGAAAGACC AGCTACTGGC AGCCAAACAT 
ATCCAATCAA AGGCCATTGA GGTTCGTCAC GCTTCCTTGA TGAAACGCTG GAATCAGCTG 
CTTGCTAATT CTGCAGCCAG GAAAAAGAAA CTCTTGGAGG CTCAGGAGCA CTTCAGAAAG 
GTTGAAGATC TCTTCTTGAC TTTTGCCAAG AAGGCCTCTG CCTTCAACAG TTGGTTTGAG 
AATGCTGAGG AGGACCTGAC GGATCCCGTG CGCTGCAATT CCCTGGAAGA AATCAAAGCA 
CTGCGAGAAG CCCACGATGC GTTCCGTTCC TCACTTAGCT CTGCCCAAGC TGACTTCAAC 
CAGCTGGCAG AGCTTGATCG GCAGATCAAG AGCTTCCGTG TAGCCTCCAA CCCGTACACT 
TGGTTTACTA TGGAGGCTCT TGAAGAAACT TGGAGGAATC TGCAGAAAAT TATCAAGGAG 
CGTGAATTGG AGTTGCAGAA GGAGCAGCGG AGGCAGGAAG AAAATGACAA GTTGCGCCAG 
GAGTTTGCTC AGCATGCTAA TGCCTTCCAC CAGTGGATTC AGGAGACAAG GACTTACCTG 
CTAGATGGGT CCTGCATGGT GGAGGAGTCG GGAACACTGG AATCCCAGCT GGAAGCTACT 
AAACGCAAGC ACCAGGAGAT CCGGGCTATG AGGAGCCAGC TGAAGAAGAT TGAGGACCTT 
GGAGCAGCCA TGGAAGAGGC ACTTATCTTG GACAACAAAT ACACAGAGCA CAGCACCGTG 
GGGCTGGCGC AGCAGTGGGA CCAGCTTGAC CAGCTGGGAA TGAGAATGCA GCACAATCTG 
GAACAGCAGA TTCAAGCTCG AAACACAACT GGAGTCACAG AGGAGGCCCT GAAGGAGTTC 
AGCATGATGT TCAAGCACTT TGACAAGGAC AAATCTGGAC GTCTTAATCA CCAGGAGTTT 
AAGTCTTGCT TGCGCTCCCT TGGCTATGAC CTGCCCATGG TTGAGGAAGG AGAGCCTGAC 
CCCGAATTTG AGTCTATTCT TGACACTGTT GATCCCAACA GGGATGGCCA CGTCTCGCTG 
CAGGAGTACA TGGCGTTCAT GATCAGCAGG GAAACAGAGA ACGTGAAATC CAGCGAGGAG 
ATTGAGAGTG CTTTCCGTGC CCTCAGCTCG GAGGGGAAGC CTTACGTGAC CAAGGAGGAG 
CTCTACCAGA ACCTGACCCG GGAGCAGGCC GACTACTGCA TTTCTCACAT GAAGCCCTAC 
ATGGATGGCA AGGGCAGAGA ACTTCCTTCT GCCTACGACT ACATAGAATT TACACGTTCA 
CTCTTTGTGA ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_015279294.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015134780.1 Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X13, mRNA.

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CloneID	OGa43320
Clone ID Related Accession (Same CDS sequence)	XM_015279294.1 , XM_015279294.2
Accession Version	XM_015279294.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015279294.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG 
TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC 
TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT 
GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT 
GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG 
ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAA CTCGTCTACA GGAGCTGCAC 
CGACTATGGG AGTTACTGCT GGAAAAAATG AGAGAGAAGG GAGTGAAGCT GTTGCAAGCA 
CAGAAGCTGG TGCAATTCTT ACGGGAATGT GAAGATGTCA TGGACTGGAT CAATGACAAG 
GAAGCAATAG TGACCTCAGA AGAGCTTGGA CAGGACTTAG AGCATGTTGA GGTTTTGCAG 
AAGAAATTTG AAGAGTTCCA AACAGATCTG GCAGCTCATG AGGAGAGAGT GAATGAAGTG 
AACCAGTTCG CTGGCAAACT TATCCAGGAA CAGCACCCTG AGGAGGAACT TATAAAGTCC 
AAACAGGATG AAGTAAATGC AAGCTGGCAG CGTCTTAAGG GGCTTGCCCA ACAGAGGCAA 
GGAAAACTCT TTGGGGCAGC TGAAGTTCAG CGCTTCAACA GGGATGTGGA TGAAACTATC 
AGCTGGATTA AGGAGAAAGG GCAGTTGATG GCCTCAGATG ACTTTGGCAG AGACTTGGCC 
AGCGTGCAAG CTTTACTACG TAAGCATGAA GGCCTGGAAA GAGATCTTGC AGCTTTGGAG 
GATAAGGTTA AGGCCCTCTG TGCAGAAGCT GACCGTTTGC AGCAGTCTCA CCCTATAAAT 
GCTTCTCAAA TTCAAGTGAA ACGGGAGGAA CTGATTGCCA ACTGGGAGCA GATCCGAACT 
CTGGCAGCAG AGAGGCATGC TCGCCTTAAT GACTCCTACA GGTTGCAGCG CTTTCTTGCA 
GATTTCCGGG ACCTCACTAG CTGGGTAACT GAGATGAAGG CTCTGATAAA TGCTGATGAG 
CTTGCCAATG ATGTGGCTGG AGCAGAAGCC CTTCTAGATA GACATCAGGA ACATAAGGGA 
GAAATTGATG CTCATGAGGA TAGCTTCAGA TCTGCTGATG AGTCTGGGCA GGCTTTGCTT 
GCAGCTGGGC ACTATGCTTC TGATGAAGTT AAAGAAAAGC TGACTATCCT CTCAGATGAA 
AGATCAGCCT TGCTGGAACT ATGGGAGCTT CGCAGGCAAC AGTATGAGCA GTGCATGGAT 
CTGCAGCTTT TCTACAGAGA TACTGAACAA GTTGACAACT GGATGAGCAA ACAAGAAGCT 
TTTCTGCTGA ACGAAGACCT TGGTGATTCT CTGGATAGCG TGGAGGCTCT TCTAAAGAAG 
CATGAAGATT TTGAGAAATC CCTAAGTGCT CAAGAGGAGA AGATCACAGC ACTGGATGAG 
TTTGCTACTA AACTGATTCA AAATAACCAT TATGCCATGG ATGACGTTGC TACGCGCAGA 
GATGCTCTTC TGAGTCGCCG AAATGCTCTT CATGAAAGAG CCATGAAGCG CCGTGCCCAA 
CTGGCAGACT CTTTCCATCT CCAGCAGTTT TTCAGGGACT CTGATGAACT GAAGAGTTGG 
GTTAATGAAA AAATGAAAAC TGCTACCGAT GAGGCTTACA AGGATCCATC CAACTTGCAA 
GGTAAAGTTC AGAAGCATCA GGCTTTTGAA GCAGAGCTTT CTGCTAATCA GAGTCGTATT 
GATGCACTGG AGAAAGCTGG CCAGAAGTTG ATTGATGTCA ATCACTATGC ATCTGATGAG 
GTGGCAGCTC GCATGAATGA AGTCATCAGC TTGTGGAAGA AACTTCTGGA AGCCACCGAG 
CTCAAAGGTA TAAAGCTGCG TGAAGCCAAT CAACAGCAGC AATTTAATCG CAATGTAGAA 
GACATTGAGT TGTGGCTGTA TGAAGTGGAG GGTCACTTGG CTTCTGATGA TTATGGAAAA 
GATCTTACCA GCGTTCAGAA TCTTCAGAAG AAACATGCCC TGCTAGAGGC AGATGTTGCT 
GCCCATCAGG ATCGGATAGA TGGCATTACC ATCCAGGCAC GCCAGTTCCA AGATGCTGGG 
CACTTTGATG CTGACAATAT CAAGAAGAAA CAAGAAGCTT TAGTAGCTCG TTATGAAGCT 
CTGAAGGATC CTATGGTGGC TCGCAAGCAG AAACTTGCAG ATTCTCTTCG CCTGCAGCAG 
CTTTTCCGTG ACATCGAGGA TGAAGAGACC TGGATCAGGG AAAAGGAACC TATTGCAGCC 
TCAACAAACC GAGGCAAGGA CTTAATTGGT GTCCAGAATC TGCTAAAGAA GCACCAGGCT 
TTGCAGGCAG AAATTGCAGG CCATGAGCCT CGCATTAAAG CAGTCACACA AAAAGGAAAT 
GCGATGGTGG AAGAGGGACA TTTTGCTGCT GAAGATGTGA AGATCAAATT GAATGAACTA 
AACCAAAAAT GGGACTCTCT GAAAGCCAAG GCATCTCAAC GGCGACAGGA TCTAGAGGAT 
TCTCTGCAAG CTCAGCAGTA TTTTGCTGAT GCTAATGAGG CAGAATCATG GATGAGGGAA 
AAGGAGCCCA TTGTAGGCAG CACGGATTAT GGAAAGGATG AAGACTCTGC TGAGGCTCTC 
CTGAAGAAGC ATGAAGCTTT GATGTCTGAT CTTTCTGCTT ACGGCAGTAG CATACAGGCA 
TTAAGGGAAC AGGCCCAGTC CTGCAGGCAA CAAGTTGCTC CCACTGATGA TGAAACTGGA 
AAAGAGCTTG TGCTAGCACT CTATGATTAC CAAGAGAAGA GTCCTCGGGA GGTGACTATG 
AAGAAAGGAG ATATTCTAAC CCTGCTCAAC AGCACCAACA AGGACTGGTG GAAGGTTGAA 
GTTAACGATC GTCAGGGCTT TGTACCAGCT GCCTATGTGA AAAAACTAGA TCCTGCCCAG 
TCTGCATCCC GAGAGAATCT TCTAGAAGAG CAAGGCAGCA TAGCATTGCG ACAGGAGCAA 
ATTGACAACC AGTATCATTC CCTTCTTGAG CTGGGAGAAA AACGTAAAGG CATGCTAGAA 
AAAAGCTGCA AGAAGTTTAT GCTTTTCCGT GAGGCTAATG AGCTTCAACA GTGGATCAAT 
GAGAAGGAAG CTGCGCTCAC TAATGAGGAA GTGGGTGCTG ATTTGGAGCA GGTGGAGGTG 
CTACAGAAGA AGTTTGATGA TTTTCAGAAG GATTTAAAAG CTAATGAGTC ACGACTGAAG 
GACATAAACA AGGTTGCAAA TGACCTGGAG TCTGAAGGGC TGATGGCAGA GGAAGTGCAA 
GCAGTAGAGC ACCAGGAAGT CTATGGAATG ATGCCCAGAG ATGAAACTGA TTCTAAGACA 
GCCTCTCCTT GGAAGTCTGC ACGTATGATG GTACACACAG TGGCAACGTT TAACTCAATC 
AAGGAACTGA ATGAACGCTG GAGATCCCTG CAGCAGTTGG CAGAGGAGCG AAGCCAGCTG 
TTGGGCAGCG CTCATGAAGT CCAGAGGTTC CACAGAGATG CTGATGAAAC AAAAGAATGG 
ATAGAAGAGA AGAATCAAGC ATTAAATACC GACAACTATG GACATGACTT GGCCAGTGTG 
CAGGCTCTGC AGCGCAAGCA TGAAGGCTTT GAGAGAGACT TGGCAGCTCT TGGAGACAAG 
GTGAATTCTC TTGGTGAAAC TGCCCAGCGT CTGATCCAGT CACATCCAGA ATCAGCTGAA 
GATCTTCAAG AAAAATGTAC TGAGTTGAAC CAAGCCTGGA GTAGTCTGGG AAAACGTGCT 
GACCAACGCA AAGAGAAGCT TGGAGATTCT CATGACCTGC AGCGTTTCCT CAGTGATTTT 
AGAGACCTTA TGTCTTGGAT CAATGGAATC CGGGGTCTGG TCTCCTCAGA TGAACTTGCA 
AAAGATGTGA CTGGAGCTGA AGCTCTGTTG GAAAGGCATC AGGAGCACCG TACTGAAATA 
GATGCACGGG CTGGCACTTT TCAGGCATTT GAACAGTTTG GACAGCAGCT TCTGGCTCGT 
GGACACTATG CCAGCCCAGA GATTAAGGAG AAACTGGATA TTCTAGATCA AGAACGGACA 
GACCTAGAGA AGGCCTGGGT CCAGCGCAGA ATGATGCTAG ACCAGTGCTT AGAACTACAG 
CTGTTTCATC GGGATTGTGA ACAAGCTGAA AACTGGATGG CTGCCCGAGA GGCGTTCCTA 
AATACAGAAG ACAAAGGAGA CTCCTTAGAC AGCGTGGAGG CACTCATCAA GAAGCATGAA 
GATTTCGATA AAGCAATCAA TGTCCAGGAA GAGAAAATTG CTGTCTTGCA GTCTTTTGCT 
GACCAACTGA TTGCTGCTGA TCATTATGCA AAAGGAGTCA TTGCTAACAG ACGCAATGAG 
GTTCTGGACA GGTGGCGTCG TCTGAAGGCT CAGATGATTG AGAAGAGATC TAAGCTGGGA 
GAATCTCAGA CCCTCCAACA GTTCAGTCGT GATGTTGATG AAATAGAAGC TTGGATCAGT 
GAAAAGCTTC AAACTGCAAG TGATGAGTCA TATAAGGATC CCACAAACAT CCAGAGCAAG 
CACCAGAAGC ACCAAGCCTT TGAAGCTGAG CTCCACGCCA ACGCAGATCG GATTCGTGGA 
GTCATTGAAA TGGGGAACTC TCTTATTGAA AGAGGAGCGT GTGCTGGCAG CGAGGATGCT 
GTGAAGGCTC GGCTGGCTGC CCTGGCTGAC CAATGGGAGT TCCTGGTCCA GAAGTCATCA 
GAGAAGAGTC AGAAACTGAA AGAAGCAAAT AAACAGCAGA ATTTCAATAC CGGCATCAAG 
GACTTTGATT TCTGGCTTTC AGAGGTGGAA GCTTTGTTGG CATCTGAAGA CTATGGGAAG 
GACTTGGCAT CAGTGAACAA CCTTCTGAAG AAGCACCAAT TACTGGAAGC TGATATATCT 
GCTCATGAGG ATCGGCTGAA GGACCTGAAC AGCCAGGCTG ACAGTTTGAT GACCAGCAGT 
GCTTTCGATA CCTCCCAAGT AAAGGATAAA CGTGAAACTA TAAATGGGCG CTTCCAGAGA 
ATCAAGAGCA TGGCAGCTGC CCGCCGTGCG AAGCTCAACG AGTCGCACCG CTTGCATCAG 
TTCTTCCGTG ACATGGATGA TGAGGAGTCC TGGATCAAAG AGAAGAAACT GTTGGTGAGC 
TCAGAGGACT ATGGCAGAGA CCTGACTGGT GTGCAGAACC TGAGGAAGAA ACATAAGCGC 
TTGGAAGCAG AATTAGCTGC CCATGAACCT GCTATCCAGG GTGTTCTAGA CACTGGCAAG 
AAGCTTTCAG ATGATAACAC AATTGGGAAG GAGGAGATAC AGCAAAGACT GGCTCAGTTT 
GTGGACCACT GGAAAGAGTT AAAACAGCTG GCAGCTGCTC GGGGGCAGCG TCTGGAGGAG 
TCCCTGGAGT ACCAACAGTT TGTAGCAAAT GTTGAGGAAG AGGAGGCATG GATCAACGAG 
AAAATGACAT TGGTAGCCAG TGAGGATTAT GGAGACACAC TTGCTGCTAT CCAGGGCTTG 
CTGAAGAAGC ATGAAGCATT CGAGACTGAC TTTACTGTCC ACAAAGACAG AGTGAATGAT 
GTTTGCGCTA ATGGAGAGGA TCTCATTAAA AAGAATAATC ACCATGTGGA GAACATTACT 
GCTAAGATGA AGGGCCTCAA GGGCAAGGTA TCAGATCTGG AGAAAGCCGC AGCTCAGAGG 
AAAGCCAAAC TGGATGAGAA CTCTGCCTTC CTCCAGTTCA ACTGGAAAGC AGATGTGGTG 
GAGTCATGGA TAGGTGAGAA GGAAAACAGT CTGAAGACAG ATGATTATGG CCGTGACCTC 
TCCTCTGTGC AAACACTACT CACCAAACAG GAAACCTTTG ATGCTGGACT TCAGGCTTTC 
CAGCAGGAGG GAATTGCAAA CATCACTGCT CTGAAAGACC AGCTACTGGC AGCCAAACAT 
ATCCAATCAA AGGCCATTGA GGTTCGTCAC GCTTCCTTGA TGAAACGCTG GAATCAGCTG 
CTTGCTAATT CTGCAGCCAG GAAAAAGAAA CTCTTGGAGG CTCAGGAGCA CTTCAGAAAG 
GTTGAAGATC TCTTCTTGAC TTTTGCCAAG AAGGCCTCTG CCTTCAACAG TTGGTTTGAG 
AATGCTGAGG AGGACCTGAC GGATCCCGTG CGCTGCAATT CCCTGGAAGA AATCAAAGCA 
CTGCGAGAAG CCCACGATGC GTTCCGTTCC TCACTTAGCT CTGCCCAAGC TGACTTCAAC 
CAGCTGGCAG AGCTTGATCG GCAGATCAAG AGCTTCCGTG TAGCCTCCAA CCCGTACACT 
TGGTTTACTA TGGAGGCTCT TGAAGAAACT TGGAGGAATC TGCAGAAAAT TATCAAGGAG 
CGTGAATTGG AGTTGCAGAA GGAGCAGCGG AGGCAGGAAG AAAATGACAA GTTGCGCCAG 
GAGTTTGCTC AGCATGCTAA TGCCTTCCAC CAGTGGATTC AGGAGACAAG GACTTACCTG 
CTAGATGGGT CCTGCATGGT GGAGGAGTCG GGAACACTGG AATCCCAGCT GGAAGCTACT 
AAACGCAAGC ACCAGGAGAT CCGGGCTATG AGGAGCCAGC TGAAGAAGAT TGAGGACCTT 
GGAGCAGCCA TGGAAGAGGC ACTTATCTTG GACAACAAAT ACACAGAGCA CAGCACCGTG 
GGGCTGGCGC AGCAGTGGGA CCAGCTTGAC CAGCTGGGAA TGAGAATGCA GCACAATCTG 
GAACAGCAGA TTCAAGCTCG AAACACAACT GGAGTCACAG AGGAGGCCCT GAAGGAGTTC 
AGCATGATGT TCAAGCACTT TGACAAGGAC AAATCTGGAC GTCTTAATCA CCAGGAGTTT 
AAGTCTTGCT TGCGCTCCCT TGGCTATGAC CTGCCCATGG TTGAGGAAGG AGAGCCTGAC 
CCCGAATTTG AGTCTATTCT TGACACTGTT GATCCCAACA GGGATGGCCA CGTCTCGCTG 
CAGGAGTACA TGGCGTTCAT GATCAGCAGG GAAACAGAGA ACGTGAAATC CAGCGAGGAG 
ATTGAGAGTG CTTTCCGTGC CCTCAGCTCG GAGGGGAAGC CTTACGTGAC CAAGGAGGAG 
CTCTACCAGA ACCTGACCCG GGAGCAGGCC GACTACTGCA TTTCTCACAT GAAGCCCTAC 
ATGGATGGCA AGGGCAGAGA ACTTCCTTCT GCCTACGACT ACATAGAATT TACACGTTCA 
CTCTTTGTGA ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015134780.1
CDS	5..7399
Translation	MLSSFRKVKVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSSLRRQKLEDSYRFQFFQRDADELEKWIQEKLQIASDENYKDPSNLQGKLQKHQAFEAEVQANSGAIVKLDETGNQMINEGHFASETIRTRLQELHRLWELLLEKMREKGVKLLQAQKLVQFLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDLAAHEERVNEVNQFAGKLIQEQHPEEELIKSKQDEVNASWQRLKGLAQQRQGKLFGAAEVQRFNRDVDETISWIKEKGQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPINASQIQVKREELIANWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFRSADESGQALLAAGHYASDEVKEKLTILSDERSALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMDDVATRRDALLSRRNALHERAMKRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYASDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTSVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDADNIKKKQEALVARYEALKDPMVARKQKLADSLRLQQLFRDIEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKIKLNELNQKWDSLKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPTDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTNEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVANDLESEGLMAEEVQAVEHQEVYGMMPRDETDSKTASPWKSARMMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAQRLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKEKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLARGHYASPEIKEKLDILDQERTDLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAVLQSFADQLIAADHYAKGVIANRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQSKHQKHQAFEAELHANADRIRGVIEMGNSLIERGACAGSEDAVKARLAALADQWEFLVQKSSEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRETINGRFQRIKSMAAARRAKLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVDHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCANGEDLIKKNNHHVENITAKMKGLKGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHIQSKAIEVRHASLMKRWNQLLANSAARKKKLLEAQEHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFESILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCISHMKPYMDGKGRELPSAYDYIEFTRSLFVN

Target ORF information:

RefSeq Version	XM_015279294.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279294.2

ORF Insert Sequence:

ATGTTGTCAT CGTTTCGTAA AGTCAAAGTC CAGAAAATGG ATCCAAGTGG GGTAAAAGTG 
TTGGAAACAG CAGAAGATAT CCAAGAACGG CGTCAGCAGG TTTTGGACCG TTACCACAGG 
TTCAAGGAGC TGTCTTCTCT GAGGCGCCAA AAACTCGAAG ATTCCTATCG GTTCCAGTTC 
TTCCAGCGCG ATGCAGATGA GCTGGAAAAA TGGATCCAAG AGAAACTCCA GATAGCATCT 
GATGAAAATT ACAAAGACCC AAGCAATTTA CAGGGGAAGC TGCAGAAGCA CCAGGCCTTT 
GAAGCTGAGG TGCAGGCCAA TTCAGGGGCT ATCGTTAAGC TGGATGAGAC TGGAAATCAG 
ATGATCAATG AAGGCCATTT TGCATCTGAA ACCATAAGAA CTCGTCTACA GGAGCTGCAC 
CGACTATGGG AGTTACTGCT GGAAAAAATG AGAGAGAAGG GAGTGAAGCT GTTGCAAGCA 
CAGAAGCTGG TGCAATTCTT ACGGGAATGT GAAGATGTCA TGGACTGGAT CAATGACAAG 
GAAGCAATAG TGACCTCAGA AGAGCTTGGA CAGGACTTAG AGCATGTTGA GGTTTTGCAG 
AAGAAATTTG AAGAGTTCCA AACAGATCTG GCAGCTCATG AGGAGAGAGT GAATGAAGTG 
AACCAGTTCG CTGGCAAACT TATCCAGGAA CAGCACCCTG AGGAGGAACT TATAAAGTCC 
AAACAGGATG AAGTAAATGC AAGCTGGCAG CGTCTTAAGG GGCTTGCCCA ACAGAGGCAA 
GGAAAACTCT TTGGGGCAGC TGAAGTTCAG CGCTTCAACA GGGATGTGGA TGAAACTATC 
AGCTGGATTA AGGAGAAAGG GCAGTTGATG GCCTCAGATG ACTTTGGCAG AGACTTGGCC 
AGCGTGCAAG CTTTACTACG TAAGCATGAA GGCCTGGAAA GAGATCTTGC AGCTTTGGAG 
GATAAGGTTA AGGCCCTCTG TGCAGAAGCT GACCGTTTGC AGCAGTCTCA CCCTATAAAT 
GCTTCTCAAA TTCAAGTGAA ACGGGAGGAA CTGATTGCCA ACTGGGAGCA GATCCGAACT 
CTGGCAGCAG AGAGGCATGC TCGCCTTAAT GACTCCTACA GGTTGCAGCG CTTTCTTGCA 
GATTTCCGGG ACCTCACTAG CTGGGTAACT GAGATGAAGG CTCTGATAAA TGCTGATGAG 
CTTGCCAATG ATGTGGCTGG AGCAGAAGCC CTTCTAGATA GACATCAGGA ACATAAGGGA 
GAAATTGATG CTCATGAGGA TAGCTTCAGA TCTGCTGATG AGTCTGGGCA GGCTTTGCTT 
GCAGCTGGGC ACTATGCTTC TGATGAAGTT AAAGAAAAGC TGACTATCCT CTCAGATGAA 
AGATCAGCCT TGCTGGAACT ATGGGAGCTT CGCAGGCAAC AGTATGAGCA GTGCATGGAT 
CTGCAGCTTT TCTACAGAGA TACTGAACAA GTTGACAACT GGATGAGCAA ACAAGAAGCT 
TTTCTGCTGA ACGAAGACCT TGGTGATTCT CTGGATAGCG TGGAGGCTCT TCTAAAGAAG 
CATGAAGATT TTGAGAAATC CCTAAGTGCT CAAGAGGAGA AGATCACAGC ACTGGATGAG 
TTTGCTACTA AACTGATTCA AAATAACCAT TATGCCATGG ATGACGTTGC TACGCGCAGA 
GATGCTCTTC TGAGTCGCCG AAATGCTCTT CATGAAAGAG CCATGAAGCG CCGTGCCCAA 
CTGGCAGACT CTTTCCATCT CCAGCAGTTT TTCAGGGACT CTGATGAACT GAAGAGTTGG 
GTTAATGAAA AAATGAAAAC TGCTACCGAT GAGGCTTACA AGGATCCATC CAACTTGCAA 
GGTAAAGTTC AGAAGCATCA GGCTTTTGAA GCAGAGCTTT CTGCTAATCA GAGTCGTATT 
GATGCACTGG AGAAAGCTGG CCAGAAGTTG ATTGATGTCA ATCACTATGC ATCTGATGAG 
GTGGCAGCTC GCATGAATGA AGTCATCAGC TTGTGGAAGA AACTTCTGGA AGCCACCGAG 
CTCAAAGGTA TAAAGCTGCG TGAAGCCAAT CAACAGCAGC AATTTAATCG CAATGTAGAA 
GACATTGAGT TGTGGCTGTA TGAAGTGGAG GGTCACTTGG CTTCTGATGA TTATGGAAAA 
GATCTTACCA GCGTTCAGAA TCTTCAGAAG AAACATGCCC TGCTAGAGGC AGATGTTGCT 
GCCCATCAGG ATCGGATAGA TGGCATTACC ATCCAGGCAC GCCAGTTCCA AGATGCTGGG 
CACTTTGATG CTGACAATAT CAAGAAGAAA CAAGAAGCTT TAGTAGCTCG TTATGAAGCT 
CTGAAGGATC CTATGGTGGC TCGCAAGCAG AAACTTGCAG ATTCTCTTCG CCTGCAGCAG 
CTTTTCCGTG ACATCGAGGA TGAAGAGACC TGGATCAGGG AAAAGGAACC TATTGCAGCC 
TCAACAAACC GAGGCAAGGA CTTAATTGGT GTCCAGAATC TGCTAAAGAA GCACCAGGCT 
TTGCAGGCAG AAATTGCAGG CCATGAGCCT CGCATTAAAG CAGTCACACA AAAAGGAAAT 
GCGATGGTGG AAGAGGGACA TTTTGCTGCT GAAGATGTGA AGATCAAATT GAATGAACTA 
AACCAAAAAT GGGACTCTCT GAAAGCCAAG GCATCTCAAC GGCGACAGGA TCTAGAGGAT 
TCTCTGCAAG CTCAGCAGTA TTTTGCTGAT GCTAATGAGG CAGAATCATG GATGAGGGAA 
AAGGAGCCCA TTGTAGGCAG CACGGATTAT GGAAAGGATG AAGACTCTGC TGAGGCTCTC 
CTGAAGAAGC ATGAAGCTTT GATGTCTGAT CTTTCTGCTT ACGGCAGTAG CATACAGGCA 
TTAAGGGAAC AGGCCCAGTC CTGCAGGCAA CAAGTTGCTC CCACTGATGA TGAAACTGGA 
AAAGAGCTTG TGCTAGCACT CTATGATTAC CAAGAGAAGA GTCCTCGGGA GGTGACTATG 
AAGAAAGGAG ATATTCTAAC CCTGCTCAAC AGCACCAACA AGGACTGGTG GAAGGTTGAA 
GTTAACGATC GTCAGGGCTT TGTACCAGCT GCCTATGTGA AAAAACTAGA TCCTGCCCAG 
TCTGCATCCC GAGAGAATCT TCTAGAAGAG CAAGGCAGCA TAGCATTGCG ACAGGAGCAA 
ATTGACAACC AGTATCATTC CCTTCTTGAG CTGGGAGAAA AACGTAAAGG CATGCTAGAA 
AAAAGCTGCA AGAAGTTTAT GCTTTTCCGT GAGGCTAATG AGCTTCAACA GTGGATCAAT 
GAGAAGGAAG CTGCGCTCAC TAATGAGGAA GTGGGTGCTG ATTTGGAGCA GGTGGAGGTG 
CTACAGAAGA AGTTTGATGA TTTTCAGAAG GATTTAAAAG CTAATGAGTC ACGACTGAAG 
GACATAAACA AGGTTGCAAA TGACCTGGAG TCTGAAGGGC TGATGGCAGA GGAAGTGCAA 
GCAGTAGAGC ACCAGGAAGT CTATGGAATG ATGCCCAGAG ATGAAACTGA TTCTAAGACA 
GCCTCTCCTT GGAAGTCTGC ACGTATGATG GTACACACAG TGGCAACGTT TAACTCAATC 
AAGGAACTGA ATGAACGCTG GAGATCCCTG CAGCAGTTGG CAGAGGAGCG AAGCCAGCTG 
TTGGGCAGCG CTCATGAAGT CCAGAGGTTC CACAGAGATG CTGATGAAAC AAAAGAATGG 
ATAGAAGAGA AGAATCAAGC ATTAAATACC GACAACTATG GACATGACTT GGCCAGTGTG 
CAGGCTCTGC AGCGCAAGCA TGAAGGCTTT GAGAGAGACT TGGCAGCTCT TGGAGACAAG 
GTGAATTCTC TTGGTGAAAC TGCCCAGCGT CTGATCCAGT CACATCCAGA ATCAGCTGAA 
GATCTTCAAG AAAAATGTAC TGAGTTGAAC CAAGCCTGGA GTAGTCTGGG AAAACGTGCT 
GACCAACGCA AAGAGAAGCT TGGAGATTCT CATGACCTGC AGCGTTTCCT CAGTGATTTT 
AGAGACCTTA TGTCTTGGAT CAATGGAATC CGGGGTCTGG TCTCCTCAGA TGAACTTGCA 
AAAGATGTGA CTGGAGCTGA AGCTCTGTTG GAAAGGCATC AGGAGCACCG TACTGAAATA 
GATGCACGGG CTGGCACTTT TCAGGCATTT GAACAGTTTG GACAGCAGCT TCTGGCTCGT 
GGACACTATG CCAGCCCAGA GATTAAGGAG AAACTGGATA TTCTAGATCA AGAACGGACA 
GACCTAGAGA AGGCCTGGGT CCAGCGCAGA ATGATGCTAG ACCAGTGCTT AGAACTACAG 
CTGTTTCATC GGGATTGTGA ACAAGCTGAA AACTGGATGG CTGCCCGAGA GGCGTTCCTA 
AATACAGAAG ACAAAGGAGA CTCCTTAGAC AGCGTGGAGG CACTCATCAA GAAGCATGAA 
GATTTCGATA AAGCAATCAA TGTCCAGGAA GAGAAAATTG CTGTCTTGCA GTCTTTTGCT 
GACCAACTGA TTGCTGCTGA TCATTATGCA AAAGGAGTCA TTGCTAACAG ACGCAATGAG 
GTTCTGGACA GGTGGCGTCG TCTGAAGGCT CAGATGATTG AGAAGAGATC TAAGCTGGGA 
GAATCTCAGA CCCTCCAACA GTTCAGTCGT GATGTTGATG AAATAGAAGC TTGGATCAGT 
GAAAAGCTTC AAACTGCAAG TGATGAGTCA TATAAGGATC CCACAAACAT CCAGAGCAAG 
CACCAGAAGC ACCAAGCCTT TGAAGCTGAG CTCCACGCCA ACGCAGATCG GATTCGTGGA 
GTCATTGAAA TGGGGAACTC TCTTATTGAA AGAGGAGCGT GTGCTGGCAG CGAGGATGCT 
GTGAAGGCTC GGCTGGCTGC CCTGGCTGAC CAATGGGAGT TCCTGGTCCA GAAGTCATCA 
GAGAAGAGTC AGAAACTGAA AGAAGCAAAT AAACAGCAGA ATTTCAATAC CGGCATCAAG 
GACTTTGATT TCTGGCTTTC AGAGGTGGAA GCTTTGTTGG CATCTGAAGA CTATGGGAAG 
GACTTGGCAT CAGTGAACAA CCTTCTGAAG AAGCACCAAT TACTGGAAGC TGATATATCT 
GCTCATGAGG ATCGGCTGAA GGACCTGAAC AGCCAGGCTG ACAGTTTGAT GACCAGCAGT 
GCTTTCGATA CCTCCCAAGT AAAGGATAAA CGTGAAACTA TAAATGGGCG CTTCCAGAGA 
ATCAAGAGCA TGGCAGCTGC CCGCCGTGCG AAGCTCAACG AGTCGCACCG CTTGCATCAG 
TTCTTCCGTG ACATGGATGA TGAGGAGTCC TGGATCAAAG AGAAGAAACT GTTGGTGAGC 
TCAGAGGACT ATGGCAGAGA CCTGACTGGT GTGCAGAACC TGAGGAAGAA ACATAAGCGC 
TTGGAAGCAG AATTAGCTGC CCATGAACCT GCTATCCAGG GTGTTCTAGA CACTGGCAAG 
AAGCTTTCAG ATGATAACAC AATTGGGAAG GAGGAGATAC AGCAAAGACT GGCTCAGTTT 
GTGGACCACT GGAAAGAGTT AAAACAGCTG GCAGCTGCTC GGGGGCAGCG TCTGGAGGAG 
TCCCTGGAGT ACCAACAGTT TGTAGCAAAT GTTGAGGAAG AGGAGGCATG GATCAACGAG 
AAAATGACAT TGGTAGCCAG TGAGGATTAT GGAGACACAC TTGCTGCTAT CCAGGGCTTG 
CTGAAGAAGC ATGAAGCATT CGAGACTGAC TTTACTGTCC ACAAAGACAG AGTGAATGAT 
GTTTGCGCTA ATGGAGAGGA TCTCATTAAA AAGAATAATC ACCATGTGGA GAACATTACT 
GCTAAGATGA AGGGCCTCAA GGGCAAGGTA TCAGATCTGG AGAAAGCCGC AGCTCAGAGG 
AAAGCCAAAC TGGATGAGAA CTCTGCCTTC CTCCAGTTCA ACTGGAAAGC AGATGTGGTG 
GAGTCATGGA TAGGTGAGAA GGAAAACAGT CTGAAGACAG ATGATTATGG CCGTGACCTC 
TCCTCTGTGC AAACACTACT CACCAAACAG GAAACCTTTG ATGCTGGACT TCAGGCTTTC 
CAGCAGGAGG GAATTGCAAA CATCACTGCT CTGAAAGACC AGCTACTGGC AGCCAAACAT 
ATCCAATCAA AGGCCATTGA GGTTCGTCAC GCTTCCTTGA TGAAACGCTG GAATCAGCTG 
CTTGCTAATT CTGCAGCCAG GAAAAAGAAA CTCTTGGAGG CTCAGGAGCA CTTCAGAAAG 
GTTGAAGATC TCTTCTTGAC TTTTGCCAAG AAGGCCTCTG CCTTCAACAG TTGGTTTGAG 
AATGCTGAGG AGGACCTGAC GGATCCCGTG CGCTGCAATT CCCTGGAAGA AATCAAAGCA 
CTGCGAGAAG CCCACGATGC GTTCCGTTCC TCACTTAGCT CTGCCCAAGC TGACTTCAAC 
CAGCTGGCAG AGCTTGATCG GCAGATCAAG AGCTTCCGTG TAGCCTCCAA CCCGTACACT 
TGGTTTACTA TGGAGGCTCT TGAAGAAACT TGGAGGAATC TGCAGAAAAT TATCAAGGAG 
CGTGAATTGG AGTTGCAGAA GGAGCAGCGG AGGCAGGAAG AAAATGACAA GTTGCGCCAG 
GAGTTTGCTC AGCATGCTAA TGCCTTCCAC CAGTGGATTC AGGAGACAAG GACTTACCTG 
CTAGATGGGT CCTGCATGGT GGAGGAGTCG GGAACACTGG AATCCCAGCT GGAAGCTACT 
AAACGCAAGC ACCAGGAGAT CCGGGCTATG AGGAGCCAGC TGAAGAAGAT TGAGGACCTT 
GGAGCAGCCA TGGAAGAGGC ACTTATCTTG GACAACAAAT ACACAGAGCA CAGCACCGTG 
GGGCTGGCGC AGCAGTGGGA CCAGCTTGAC CAGCTGGGAA TGAGAATGCA GCACAATCTG 
GAACAGCAGA TTCAAGCTCG AAACACAACT GGAGTCACAG AGGAGGCCCT GAAGGAGTTC 
AGCATGATGT TCAAGCACTT TGACAAGGAC AAATCTGGAC GTCTTAATCA CCAGGAGTTT 
AAGTCTTGCT TGCGCTCCCT TGGCTATGAC CTGCCCATGG TTGAGGAAGG AGAGCCTGAC 
CCCGAATTTG AGTCTATTCT TGACACTGTT GATCCCAACA GGGATGGCCA CGTCTCGCTG 
CAGGAGTACA TGGCGTTCAT GATCAGCAGG GAAACAGAGA ACGTGAAATC CAGCGAGGAG 
ATTGAGAGTG CTTTCCGTGC CCTCAGCTCG GAGGGGAAGC CTTACGTGAC CAAGGAGGAG 
CTCTACCAGA ACCTGACCCG GGAGCAGGCC GACTACTGCA TTTCTCACAT GAAGCCCTAC 
ATGGATGGCA AGGGCAGAGA ACTTCCTTCT GCCTACGACT ACATAGAATT TACACGTTCA 
CTCTTTGTGA ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( NM_001042538.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001036003.1 Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), mRNA.

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CloneID	OGa27423
Clone ID Related Accession (Same CDS sequence)	NM_001042538.1
Accession Version	NM_001042538.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7434bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-22
Organism	Gallus gallus(chicken)
Product	spectrin alpha chain, non-erythrocytic 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CN217639.1 and X14519.1. On Jul 14, 2006 this sequence version replaced XM_415495.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.

ATGGATCCAA GTGGGGTAAA AGTGTTGGAA ACAGCAGAAG ATATCCAAGA ACGGCGTCAG 
CAGGTTTTGG ACCGTTACCA CAGGTTCAAG GAGCTGTCTT CTCTGAGGCG CCAAAAACTC 
GAAGATTCCT ATCGGTTCCA GTTCTTCCAG CGTGATGCAG ATGAGCTGGG AAAATGGATC 
CAAGAGAAAC TCCAGATAGC ATCTGATGAA AATTACAAAG ACCCAAGCAA TTTACAGGGG 
AAGCTGCAGA AGCACCAGGC CTTTGAAGCT GAGGTGCAGG CCAATTCAGG GGCTATCGTT 
AAGCTGGATG AGACTGGAAA TCAGATGATC AATGAAGGCC ATTTTGCATC TGAAACCATA 
AGAACTCGTC TACAGGAGCT GCACCGACTA TGGGAGTTAC TGCTGGAAAA AATGAGAGAG 
AAGGGAGTGA AGCTGTTGCA AGCACAGAAG CTGGTGCAAT TCTTACGGGA ATGTGAAGAT 
GTCATGGACT GGATCAATGA CAAGGAAGCA ATAGTGACCT CAGAAGAGCT TGGACAGGAC 
TTAGAGCATG TTGAGGTTTT GCAGAAGAAA TTTGAAGAGT TCCAAACAGA TCTGGCAGCT 
CATGAGGAGA GAGTGAATGA AGTGAACCAG TTCGCTGGGA AACTTATCCA GGAACAGCAC 
CCTGAGGAGG AACTTATAAA GTCCAAACAG GATGAAGTAA ATGCAAGCTG GCAGCGTCTT 
AAGGGGCTTG CCCAACAGAG GCAAGGAAAA CTCTTTGGGG CAGCTGAAGT TCAGCGCTTC 
AACAGGGATG TGGATGAAAC TATCAGCTGG ATTAAGGAGA AAGGGCAGTT GATGGCCTCA 
GATGACTTTG GCAGAGACTT GGCCAGCGTG CAAGCTTTAC TACGTAAGCA TGAAGGCCTG 
GAAAGAGATC TTGCAGCTTT CCACCATAAG GTTAAGGCCC TCTGTGCAGA AGCTGACCGT 
TTGCAGCAAT CTCACCCTAT AAATGCTTCT CAAATTCAAG TGAAACGGGA GGAACTGATT 
GCCAACTGGG AGCAGATCCG AACTCTGGCA GCAGAGAGGC ATGCTCGCCT TAATGACTCC 
TACAGGTTGC AGCGCTTTCT TGCAGATTTC CGGGACCTCA CTAGCTGGGT AACTGAGATG 
AAGGCTCTGA TAAATGCTGA TGAGCTTGCC AATGATGTGG CTGGAGCAGA AGCCCTTCTA 
GATAGACATC AGGAACATAA GGGAGAAATT GATGCTCATG AGGATAGCTT CAGATCTGCT 
GATGAGTCTG GGCAGGCTTT GCTTGCAGCT GGGCACTATG CTTCTGATGA AGTTAAAGAA 
AAGCTGACTA TCCTCTCAGA TGAAAGATCT GCCTTGCTGG AACTATGGGA GCTTCGCAGG 
CAACAGTATG AGCAGTGCAT GGATCTGCAG CTTTTCTACA GAGATACTGA ACAAGTTGAC 
AACTGGATGA GCAAACAAGA AGCTTTTCTG CTGAACGAAG ACCTTGGTGA TTCTCTGGAT 
AGCGTGGAGG CTCTTCTAAA GAAGCATGAA GATTTTGAGA AATCCCTAAG TGCTCAAGAG 
GAGAAAATCA CAGCACTGGA TGAGTTTGCT ACTAAACTGA TTCAAAATAA CCATTATGCC 
ATGGATGACG TTGCTACGCG CAGAGATGCT CTTCTGAGTC GCCGAAATGC TCTTCATGAA 
AGAGCCATGA AGCGCCGTGC CCAACTGGCA GACTCTTTCC ATCTCCAGCA GTTTTTCAGG 
GACTCTGATG AACTGAAGAG TTGGGTTAAT GAAAAAATGA AAACTGCTAC CGATGAGGCT 
TACAAGGATC CATCCAACTT GCAAGGTAAA GTTCAGAAGC ATCAGGCTTT TGAAGCAGAG 
CTTTCTGCTA ATCAGAGTCG TATTGATGCA CTGGAGAAAG CTGGCCAGAA GTTGATTGAT 
GTCAATCACT ATGCATCTGA TGAGGTGGCA GCTCGCATGA ATGAAGTCAT CAGCTTGTGG 
AAGAAACTTC TGGAAGCCAC CGAGCTCAAA GGTATAAAGC TGCGTGAAGC CAATCAACAG 
CAGCAATTTA ATCGCAATGT AGAAGACATT GAGTTGTGGC TGTATGAAGT GGAGGGTCAC 
TTGGCTTCTG ATGATTATGG AAAAGATCTT ACCAGCGTTC AGAATCTTCA GAAGAAACAT 
GCCCTGCTAG AGGCAGATGT TGCTGCCCAT CAGGATCCGA TAGATGGCAT TACCATCCAG 
GCACGCCAGT TCCAAGATGC TGGGCACTTT GATGCTGACA ATATCAAGAA GAAACAAGAA 
GCTTTAGTAG CTCGTTATGA AGCTCTGAAG GATCCTATGG TGGCTCGCAA GCAGAAACTT 
GCAGATTCTC TTCGCCTGCA GCAGCTTTTC CGTGACATCG AGGATGAAGA GACCTGGATC 
AGGGAAAAGG AACCTATTGC AGCCTCAACA AACCGAGGCA AGGACTTAAT TGGTGTCCAG 
AATCTGCTAA AGAAGCACCA GGCTTTGCAG GCAGAAATTG CAGGCCATGA GCCTCGCATT 
AAAGCAGTCA CACAAAAAGG AAATGCGATG GTGGAAGAGG GACATTTTGC TGCTGAAGAT 
GTGAAGATCA AATTGAATGA ACTAAACCAA AAATGGGACT CTCTGAAAGC GAAGGCATCT 
CAACGGCGAC AGGATCTAGA GGATTCTCTG CAAGCTCAGC AGTATTTTGC TGATGCTAAT 
GAGGCACAAT CATGGATGAG GGAAAAGGAG CCCATTGTAG GCAGCACGGA TTATGGAAAG 
GATGAAGACT CTGCTGAGGC TCTCCTGAAG AAGCATGAAG CTTTGATGTC TGATCTTTCT 
GCTTACGGCA GTAGCATACA GGCATTAAGG GAACAGGCCC AGTCCTGCAG GCAACAAGTT 
GCTCCCACTG ATGATGAAAC TGGAAAAGAG CTTGTGCTAG CACTCTATGA TTACCAAGAG 
AAGAGTCCTC GGGAGGTGAC TATGAAGAAA GGAGATATTC TAACCCTGCT CAACAGCACC 
AACAAGGACT GGTGGAAGGT TGAAGTTAAC GATCGTCAGG GCTTTGTACC AGCTGCCTAT 
GTGAAAAAAC TAGATCCTGC CCAGTCTGCA TCCCGAGAGA ATCTTCTGGA AGAGCAAGGC 
AGCATAGCAT TGCGACAGGA GCAAATTGAC AACCAGACTC TCATTACTAA GGAAGTCGGC 
AGTGTATCTC TGCGTATGAA ACAGGTCGAA GAACTGTATC ATTCCCTTCT TGAGCTGGGA 
GAAAAACGTA AAGGCATGCT AGAAAAAAGC TGCAAGAAGT TTATGCTTTT CCGTGAGGCT 
AATGAGCTTC AACAGTGGAT CAATGAGAAG GAAGCTGCAC TCACTAACGA GGAAGTGGGT 
GCTGATTTGG AGCAGGTGGA GGTGCTACAG AAGAAGTTTG ATGATTTTCA GAAGGATTTA 
AAAGCTAATG AGTCACGACT GAAGGACATA AACAAGGTTG CAAATGATCT GGAGTCTGAA 
GGGCTGATGG CAGAGGAAGT GCAAGCAGTA GAGCACCAGG AAGTCTATGG AATGATGCCC 
AGAGATGAAA CTGATTCTAA GACAGTCTCT CCTTGGAAGT CTGCACGTAT GATGGTACAC 
ACGGTGGCAA CATTTAACTC AATTAAGGAA CTGAATGAAC GCTGGAGATC CCTGCAGCAG 
TTGGCAGAGG AGCGAAGCCA GCTGTTGGGC AGCGCTGATG AAGTCCAGAG GTTCCACAGA 
GATGCTGATG AAACAAAAGA ATGGATAGAA GAGAAGAATC AAGCATTAAA TACCGACAAC 
TATGGACATG ACTTGGCCAG TGTGCAGGCT CTGCAGCGCA ACGATGAAGG CTTTGAGAGA 
GACTTGGCAG CTCTTGGAGA CAAGGTGAAT TCTCTTGGTG AAACTGCCCA GCGTCTGATC 
CAGTCACATC CAGAACTAGC TGAAGATCTT CAAGAAAAAT GTACTGAGTT GAACCAAGCC 
TGGAGTAGTC TGGGAAAACG TGCTGACCAA CGCAAAGAGA AGCTTGGAGA TTCTCATGAC 
CTGCAGCGTT TCCTCAGTGA TTTTAGAGAC CTTATGTCTT GGATCAATGG AATCCGGGGT 
CTGGTCTCCT CAGATGAACT TGCAAAAGAT GTGACTGGAG CTGAAGCTCT GTTGGAAAGG 
CATCAGGAGC ACCGTACTGA AATAGATGCA CGGGCTGGCA CTTTTCAGGC ATTTGAACAG 
TTTGGACAGC AGCTTCTGGC TCGTGGACAC TATGCCAGCC CAGAGATTAA GGAGAAACTG 
GATATTCTAG ATCAAGAACG GACAGACCTA GAGAAGGCCT GGGTCCAGCG CAGAATGATG 
CTAGACCAGT GCTTAGAACT ACAGCTGTTT CATCGGGATT GTGAACAAGC TGAAAACTGG 
ATGGCTGCCC GAGAGGCGTT CCTAAATACA GAAGACAAAG GAGACTCCTT AGACAGCGTG 
GAGGCACTCA TCAAGAAGCA TGAAGATTTC GATAAAGCAA TCAATGTCCA GGAAGAGAAA 
ATTGCTGTCT TGCAGTCTTT TGCTGACCAA CTGATTGCTG CTGATCATTA TGCAAAAGGA 
GTCATTGCTA ACAGACGCAA TGAGGTTCTG GACAGGTGGC GTCGTCTGAA GGCTCAGATG 
ATTGAGAAGA GATCTAAGCT GGGAGAATCT CAGACCCTCC AACAGTTCAG TCGTGATGTT 
GATGAAATAG AAGCTTGGAT CAGTGAAAAG CTTCAAACTG CAAGTGATGA GTCATATAAG 
GATCCCACAA ACATCCAGCT TTCCAAACTG CTGAGCAAGC ACCAGAAGCA CCAAGCCTTT 
GAAGCTGAGC TCCACGCCAA CGCAGATCGG ATTCGTGGAG TCATTGAAAT GGGGAACCCT 
CTTATTGAAA GAGGAGCGTG TGCTGGCAGC GAGGATGCTG TGAAGGCTCG GCTGGCTGCC 
CTGGCTGACC AATGGGAGTT CCTGGTCCAG AAGTCATCAG AGAAGAGTCA GAAACTGAAA 
GAAGCAAATA AACAGCAGAA TTTCAATACC GGCATCAAGG ACTTTGATTT CTGGCTTTCA 
GAGGTGGAAG CTTTGTTGGC ATCTGAAGAC TATGGGAAGG ACTTGGCATC AGTGAACAAC 
CTTCTGAAGA AGCACCAATT ACTGGAAGCT GATATATCTG CTCATGAGGA TCGGCTGAAG 
GACCTGAACA GCCAGGCTGA CAGTTTGATG ACCAGCAGTG CTTTCGATAC CTCCCAAGTA 
AAGGATAAAC GTGAAACTAT AAATGGGCGC TTCCAGAGAA TCAAGAGCAT GGCAGCTGCC 
CGCCGTGCGA AGCTCAACGA GTCGCACCGC TTGCATCAGT TCTTCCGTGA CATGGATGAT 
GAGGAGTCCT GGATCAAAGA GAAGAAACTG TTGGTGAGCT CAGAGGACTA TGGCAGAGAC 
CTGACTGGTG TGCAGAACCT GAGGAAGAAA CATAAGCGCT TGGAAGCAGA ATTAGCTGCC 
CATGAACCTG CTATCCAGGG TGTTCTAGAC ACTGGCAAGA AGCTTTCAGA TGATAACACA 
ATTGGGAAGG AGGAGATACA GCAAAGACTG GCTCAGTTTG TGGACCACTG GAAAGAGTTA 
AAACAGCTGG CAGCTGCTCG GGGGCAACGT CTGGAGGAGT CCCTGGAGTA CCAACAGTTT 
GTAGCAAATG TTGAGGAAGA GGAGGCATGG ATCAACGAGA AAATGACATT GGTAGCCAGT 
GAGGATTATG GAGACACACT TGCTGCTATC CAGGGCTTGC TGAAGAAGCA TGAAGCATTC 
GAGACTGACT TTACTGTCCA CAAAGACAGA GTGAATGATG TTTGTGCTAA TGGAGAGGAT 
CTCATTAAAA AGAATAATCA CCATGTGGAG AACATTACTG CTAAGATGAA GGGCCTCAAG 
GGCAAGGTAT CAGATCTGGA GAAAGCCGCA GCTCAGAGGA AAGCCAAACT GGATGAGAAC 
TCTGCCTTCC TCCAGTTCAA CTGGAAAGCA GATGTGGTGG AGTCATGGAT AGGTGAGAAG 
GAAAACAGCC TGAAGACAGA TGATTATGGC CGTGACCTCT CCTCTGTGCA AACACTACTC 
ACCAAACAGG AAACCTTTGA TGCTGGACTT CAGGCTTTCC AGCAGGAGGG AATTGCAAAC 
ATCACTGCTC TGAAAGACCA GCTACTGGCA GCCAAACATA TCCAATCAAA GGCCATTGAG 
GTTCGTCACG CTTCCTTGAT GAAACGCTGG AATCAGCTGC TTGCTAATTC TGCAGCCAGG 
AAAAAGAAAC TCTTGGAGGC TCAGGAGCAC TTCAGAAAGG TTGAAGATCT CTTCTTGACT 
TTTGCCAAGA AGGCCTCTGC CTTCAACAGT TGGTTTGAGA ATGCTGAGGA GGACCTGACG 
GATCCCGTGC GCTGCAATTC CCTGGAAGAA ATCAAAGCAC TGCGAGAAGC CCACGATGCC 
TTCCGTTCCT CACTTAGCTC TGCCCAAGCT GACTTCAACC AGCTGGCAGA GCTTGATCGG 
CAGATCAAGA GCTTCCGTGT AGCCTCCAAC CCGTACACTT GGTTTACTAT GGAGGCTCTT 
GAAGAAACTT GGAGGAATCT GCAGAAAATT ATCAAGGAGC GTGAATTGGA GTTGCAGAAG 
GAGCAGCGGA GGCAGGAAGA AAATGACAAG TTGCGCCAGG AGTTTGCTCA GCATGCTAAT 
GCCTTCCACC AGTGGATTCA GGAGACAAGG ACTTACCTGC TAGATGGGTC CTGTATGGTG 
GAGGAGTCGG GAACACTGGA ATCCCAGCTG GAAGCTACTA AACGCAAGCA CCAGGAGATC 
CGGGCTATGA GGAGCCAGCT GAAGAAGATT GAGGACCTTG GAGCAGCCAT GGAAGAGGCA 
CTTATCTTGG ACAACAAATA CACAGAGCAC AGCACCGTGG GGCTGGCGCA GCAGTGGGAC 
CAGCTTGACC AGCTGGGAAT GAGAATGCAG CACAATCTGG AACAGCAGAT TCAAGCTCGA 
AACACAACTG GAGTCACAGA GGAGGCCCTG AAGGAGTTCA GCATGATGTT CAAGCACTTT 
GACAAGGACA AATCTGGACG TCTTAATCAC CAGGAGTTTA AGTCTTGCTT GCGCTCCCTT 
GGCTATGACC TGCCCATGGT TGAGGAAGGA GAGCCTGACC CCGAATTTGA GTCTATTCTT 
GACACTGTTG ATCCCAACAG GGATGGCCAC GTCTCGCTGC AGGAGTACAT GGCGTTCATG 
ATCAGCAGGG AAACAGAGAA CGTGAAATCC AGCGAGGAGA TTGAGAGTGC TTTCCGTGCC 
CTCAGCTCGG AGAGGAAGCC TTACGTGACC AAGGAGGAGC TCTACCAGAA CCTGACCCGG 
GAGCAGGCCG ACTACTGCAT TTCTCACATG AAGCCCTACA TGGATGGCAA GGGCAGAGAA 
CTTCCTTCTG CCTACGACTA CATAGAATTT ACACGTTCAC TCTTTGTGAA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001036003.1
CDS	20..7453
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSSLRRQKLEDSYRFQFFQRDADELGKWIQEKLQIASDENYKDPSNLQGKLQKHQAFEAEVQANSGAIVKLDETGNQMINEGHFASETIRTRLQELHRLWELLLEKMREKGVKLLQAQKLVQFLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDLAAHEERVNEVNQFAGKLIQEQHPEEELIKSKQDEVNASWQRLKGLAQQRQGKLFGAAEVQRFNRDVDETISWIKEKGQLMASDDFGRDLASVQALLRKHEGLERDLAAFHHKVKALCAEADRLQQSHPINASQIQVKREELIANWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFRSADESGQALLAAGHYASDEVKEKLTILSDERSALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMDDVATRRDALLSRRNALHERAMKRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYASDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTSVQNLQKKHALLEADVAAHQDPIDGITIQARQFQDAGHFDADNIKKKQEALVARYEALKDPMVARKQKLADSLRLQQLFRDIEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKIKLNELNQKWDSLKAKASQRRQDLEDSLQAQQYFADANEAQSWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPTDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTLITKEVGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTNEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVANDLESEGLMAEEVQAVEHQEVYGMMPRDETDSKTVSPWKSARMMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSADEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRNDEGFERDLAALGDKVNSLGETAQRLIQSHPELAEDLQEKCTELNQAWSSLGKRADQRKEKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLARGHYASPEIKEKLDILDQERTDLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAVLQSFADQLIAADHYAKGVIANRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIEMGNPLIERGACAGSEDAVKARLAALADQWEFLVQKSSEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRETINGRFQRIKSMAAARRAKLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVDHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCANGEDLIKKNNHHVENITAKMKGLKGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHIQSKAIEVRHASLMKRWNQLLANSAARKKKLLEAQEHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFESILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSERKPYVTKEELYQNLTREQADYCISHMKPYMDGKGRELPSAYDYIEFTRSLFVN

Target ORF information:

RefSeq Version	NM_001042538.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus spectrin alpha, non-erythrocytic 1 (SPTAN1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001042538.1

ORF Insert Sequence:

ATGGATCCAA GTGGGGTAAA AGTGTTGGAA ACAGCAGAAG ATATCCAAGA ACGGCGTCAG 
CAGGTTTTGG ACCGTTACCA CAGGTTCAAG GAGCTGTCTT CTCTGAGGCG CCAAAAACTC 
GAAGATTCCT ATCGGTTCCA GTTCTTCCAG CGTGATGCAG ATGAGCTGGG AAAATGGATC 
CAAGAGAAAC TCCAGATAGC ATCTGATGAA AATTACAAAG ACCCAAGCAA TTTACAGGGG 
AAGCTGCAGA AGCACCAGGC CTTTGAAGCT GAGGTGCAGG CCAATTCAGG GGCTATCGTT 
AAGCTGGATG AGACTGGAAA TCAGATGATC AATGAAGGCC ATTTTGCATC TGAAACCATA 
AGAACTCGTC TACAGGAGCT GCACCGACTA TGGGAGTTAC TGCTGGAAAA AATGAGAGAG 
AAGGGAGTGA AGCTGTTGCA AGCACAGAAG CTGGTGCAAT TCTTACGGGA ATGTGAAGAT 
GTCATGGACT GGATCAATGA CAAGGAAGCA ATAGTGACCT CAGAAGAGCT TGGACAGGAC 
TTAGAGCATG TTGAGGTTTT GCAGAAGAAA TTTGAAGAGT TCCAAACAGA TCTGGCAGCT 
CATGAGGAGA GAGTGAATGA AGTGAACCAG TTCGCTGGGA AACTTATCCA GGAACAGCAC 
CCTGAGGAGG AACTTATAAA GTCCAAACAG GATGAAGTAA ATGCAAGCTG GCAGCGTCTT 
AAGGGGCTTG CCCAACAGAG GCAAGGAAAA CTCTTTGGGG CAGCTGAAGT TCAGCGCTTC 
AACAGGGATG TGGATGAAAC TATCAGCTGG ATTAAGGAGA AAGGGCAGTT GATGGCCTCA 
GATGACTTTG GCAGAGACTT GGCCAGCGTG CAAGCTTTAC TACGTAAGCA TGAAGGCCTG 
GAAAGAGATC TTGCAGCTTT CCACCATAAG GTTAAGGCCC TCTGTGCAGA AGCTGACCGT 
TTGCAGCAAT CTCACCCTAT AAATGCTTCT CAAATTCAAG TGAAACGGGA GGAACTGATT 
GCCAACTGGG AGCAGATCCG AACTCTGGCA GCAGAGAGGC ATGCTCGCCT TAATGACTCC 
TACAGGTTGC AGCGCTTTCT TGCAGATTTC CGGGACCTCA CTAGCTGGGT AACTGAGATG 
AAGGCTCTGA TAAATGCTGA TGAGCTTGCC AATGATGTGG CTGGAGCAGA AGCCCTTCTA 
GATAGACATC AGGAACATAA GGGAGAAATT GATGCTCATG AGGATAGCTT CAGATCTGCT 
GATGAGTCTG GGCAGGCTTT GCTTGCAGCT GGGCACTATG CTTCTGATGA AGTTAAAGAA 
AAGCTGACTA TCCTCTCAGA TGAAAGATCT GCCTTGCTGG AACTATGGGA GCTTCGCAGG 
CAACAGTATG AGCAGTGCAT GGATCTGCAG CTTTTCTACA GAGATACTGA ACAAGTTGAC 
AACTGGATGA GCAAACAAGA AGCTTTTCTG CTGAACGAAG ACCTTGGTGA TTCTCTGGAT 
AGCGTGGAGG CTCTTCTAAA GAAGCATGAA GATTTTGAGA AATCCCTAAG TGCTCAAGAG 
GAGAAAATCA CAGCACTGGA TGAGTTTGCT ACTAAACTGA TTCAAAATAA CCATTATGCC 
ATGGATGACG TTGCTACGCG CAGAGATGCT CTTCTGAGTC GCCGAAATGC TCTTCATGAA 
AGAGCCATGA AGCGCCGTGC CCAACTGGCA GACTCTTTCC ATCTCCAGCA GTTTTTCAGG 
GACTCTGATG AACTGAAGAG TTGGGTTAAT GAAAAAATGA AAACTGCTAC CGATGAGGCT 
TACAAGGATC CATCCAACTT GCAAGGTAAA GTTCAGAAGC ATCAGGCTTT TGAAGCAGAG 
CTTTCTGCTA ATCAGAGTCG TATTGATGCA CTGGAGAAAG CTGGCCAGAA GTTGATTGAT 
GTCAATCACT ATGCATCTGA TGAGGTGGCA GCTCGCATGA ATGAAGTCAT CAGCTTGTGG 
AAGAAACTTC TGGAAGCCAC CGAGCTCAAA GGTATAAAGC TGCGTGAAGC CAATCAACAG 
CAGCAATTTA ATCGCAATGT AGAAGACATT GAGTTGTGGC TGTATGAAGT GGAGGGTCAC 
TTGGCTTCTG ATGATTATGG AAAAGATCTT ACCAGCGTTC AGAATCTTCA GAAGAAACAT 
GCCCTGCTAG AGGCAGATGT TGCTGCCCAT CAGGATCCGA TAGATGGCAT TACCATCCAG 
GCACGCCAGT TCCAAGATGC TGGGCACTTT GATGCTGACA ATATCAAGAA GAAACAAGAA 
GCTTTAGTAG CTCGTTATGA AGCTCTGAAG GATCCTATGG TGGCTCGCAA GCAGAAACTT 
GCAGATTCTC TTCGCCTGCA GCAGCTTTTC CGTGACATCG AGGATGAAGA GACCTGGATC 
AGGGAAAAGG AACCTATTGC AGCCTCAACA AACCGAGGCA AGGACTTAAT TGGTGTCCAG 
AATCTGCTAA AGAAGCACCA GGCTTTGCAG GCAGAAATTG CAGGCCATGA GCCTCGCATT 
AAAGCAGTCA CACAAAAAGG AAATGCGATG GTGGAAGAGG GACATTTTGC TGCTGAAGAT 
GTGAAGATCA AATTGAATGA ACTAAACCAA AAATGGGACT CTCTGAAAGC GAAGGCATCT 
CAACGGCGAC AGGATCTAGA GGATTCTCTG CAAGCTCAGC AGTATTTTGC TGATGCTAAT 
GAGGCACAAT CATGGATGAG GGAAAAGGAG CCCATTGTAG GCAGCACGGA TTATGGAAAG 
GATGAAGACT CTGCTGAGGC TCTCCTGAAG AAGCATGAAG CTTTGATGTC TGATCTTTCT 
GCTTACGGCA GTAGCATACA GGCATTAAGG GAACAGGCCC AGTCCTGCAG GCAACAAGTT 
GCTCCCACTG ATGATGAAAC TGGAAAAGAG CTTGTGCTAG CACTCTATGA TTACCAAGAG 
AAGAGTCCTC GGGAGGTGAC TATGAAGAAA GGAGATATTC TAACCCTGCT CAACAGCACC 
AACAAGGACT GGTGGAAGGT TGAAGTTAAC GATCGTCAGG GCTTTGTACC AGCTGCCTAT 
GTGAAAAAAC TAGATCCTGC CCAGTCTGCA TCCCGAGAGA ATCTTCTGGA AGAGCAAGGC 
AGCATAGCAT TGCGACAGGA GCAAATTGAC AACCAGACTC TCATTACTAA GGAAGTCGGC 
AGTGTATCTC TGCGTATGAA ACAGGTCGAA GAACTGTATC ATTCCCTTCT TGAGCTGGGA 
GAAAAACGTA AAGGCATGCT AGAAAAAAGC TGCAAGAAGT TTATGCTTTT CCGTGAGGCT 
AATGAGCTTC AACAGTGGAT CAATGAGAAG GAAGCTGCAC TCACTAACGA GGAAGTGGGT 
GCTGATTTGG AGCAGGTGGA GGTGCTACAG AAGAAGTTTG ATGATTTTCA GAAGGATTTA 
AAAGCTAATG AGTCACGACT GAAGGACATA AACAAGGTTG CAAATGATCT GGAGTCTGAA 
GGGCTGATGG CAGAGGAAGT GCAAGCAGTA GAGCACCAGG AAGTCTATGG AATGATGCCC 
AGAGATGAAA CTGATTCTAA GACAGTCTCT CCTTGGAAGT CTGCACGTAT GATGGTACAC 
ACGGTGGCAA CATTTAACTC AATTAAGGAA CTGAATGAAC GCTGGAGATC CCTGCAGCAG 
TTGGCAGAGG AGCGAAGCCA GCTGTTGGGC AGCGCTGATG AAGTCCAGAG GTTCCACAGA 
GATGCTGATG AAACAAAAGA ATGGATAGAA GAGAAGAATC AAGCATTAAA TACCGACAAC 
TATGGACATG ACTTGGCCAG TGTGCAGGCT CTGCAGCGCA ACGATGAAGG CTTTGAGAGA 
GACTTGGCAG CTCTTGGAGA CAAGGTGAAT TCTCTTGGTG AAACTGCCCA GCGTCTGATC 
CAGTCACATC CAGAACTAGC TGAAGATCTT CAAGAAAAAT GTACTGAGTT GAACCAAGCC 
TGGAGTAGTC TGGGAAAACG TGCTGACCAA CGCAAAGAGA AGCTTGGAGA TTCTCATGAC 
CTGCAGCGTT TCCTCAGTGA TTTTAGAGAC CTTATGTCTT GGATCAATGG AATCCGGGGT 
CTGGTCTCCT CAGATGAACT TGCAAAAGAT GTGACTGGAG CTGAAGCTCT GTTGGAAAGG 
CATCAGGAGC ACCGTACTGA AATAGATGCA CGGGCTGGCA CTTTTCAGGC ATTTGAACAG 
TTTGGACAGC AGCTTCTGGC TCGTGGACAC TATGCCAGCC CAGAGATTAA GGAGAAACTG 
GATATTCTAG ATCAAGAACG GACAGACCTA GAGAAGGCCT GGGTCCAGCG CAGAATGATG 
CTAGACCAGT GCTTAGAACT ACAGCTGTTT CATCGGGATT GTGAACAAGC TGAAAACTGG 
ATGGCTGCCC GAGAGGCGTT CCTAAATACA GAAGACAAAG GAGACTCCTT AGACAGCGTG 
GAGGCACTCA TCAAGAAGCA TGAAGATTTC GATAAAGCAA TCAATGTCCA GGAAGAGAAA 
ATTGCTGTCT TGCAGTCTTT TGCTGACCAA CTGATTGCTG CTGATCATTA TGCAAAAGGA 
GTCATTGCTA ACAGACGCAA TGAGGTTCTG GACAGGTGGC GTCGTCTGAA GGCTCAGATG 
ATTGAGAAGA GATCTAAGCT GGGAGAATCT CAGACCCTCC AACAGTTCAG TCGTGATGTT 
GATGAAATAG AAGCTTGGAT CAGTGAAAAG CTTCAAACTG CAAGTGATGA GTCATATAAG 
GATCCCACAA ACATCCAGCT TTCCAAACTG CTGAGCAAGC ACCAGAAGCA CCAAGCCTTT 
GAAGCTGAGC TCCACGCCAA CGCAGATCGG ATTCGTGGAG TCATTGAAAT GGGGAACCCT 
CTTATTGAAA GAGGAGCGTG TGCTGGCAGC GAGGATGCTG TGAAGGCTCG GCTGGCTGCC 
CTGGCTGACC AATGGGAGTT CCTGGTCCAG AAGTCATCAG AGAAGAGTCA GAAACTGAAA 
GAAGCAAATA AACAGCAGAA TTTCAATACC GGCATCAAGG ACTTTGATTT CTGGCTTTCA 
GAGGTGGAAG CTTTGTTGGC ATCTGAAGAC TATGGGAAGG ACTTGGCATC AGTGAACAAC 
CTTCTGAAGA AGCACCAATT ACTGGAAGCT GATATATCTG CTCATGAGGA TCGGCTGAAG 
GACCTGAACA GCCAGGCTGA CAGTTTGATG ACCAGCAGTG CTTTCGATAC CTCCCAAGTA 
AAGGATAAAC GTGAAACTAT AAATGGGCGC TTCCAGAGAA TCAAGAGCAT GGCAGCTGCC 
CGCCGTGCGA AGCTCAACGA GTCGCACCGC TTGCATCAGT TCTTCCGTGA CATGGATGAT 
GAGGAGTCCT GGATCAAAGA GAAGAAACTG TTGGTGAGCT CAGAGGACTA TGGCAGAGAC 
CTGACTGGTG TGCAGAACCT GAGGAAGAAA CATAAGCGCT TGGAAGCAGA ATTAGCTGCC 
CATGAACCTG CTATCCAGGG TGTTCTAGAC ACTGGCAAGA AGCTTTCAGA TGATAACACA 
ATTGGGAAGG AGGAGATACA GCAAAGACTG GCTCAGTTTG TGGACCACTG GAAAGAGTTA 
AAACAGCTGG CAGCTGCTCG GGGGCAACGT CTGGAGGAGT CCCTGGAGTA CCAACAGTTT 
GTAGCAAATG TTGAGGAAGA GGAGGCATGG ATCAACGAGA AAATGACATT GGTAGCCAGT 
GAGGATTATG GAGACACACT TGCTGCTATC CAGGGCTTGC TGAAGAAGCA TGAAGCATTC 
GAGACTGACT TTACTGTCCA CAAAGACAGA GTGAATGATG TTTGTGCTAA TGGAGAGGAT 
CTCATTAAAA AGAATAATCA CCATGTGGAG AACATTACTG CTAAGATGAA GGGCCTCAAG 
GGCAAGGTAT CAGATCTGGA GAAAGCCGCA GCTCAGAGGA AAGCCAAACT GGATGAGAAC 
TCTGCCTTCC TCCAGTTCAA CTGGAAAGCA GATGTGGTGG AGTCATGGAT AGGTGAGAAG 
GAAAACAGCC TGAAGACAGA TGATTATGGC CGTGACCTCT CCTCTGTGCA AACACTACTC 
ACCAAACAGG AAACCTTTGA TGCTGGACTT CAGGCTTTCC AGCAGGAGGG AATTGCAAAC 
ATCACTGCTC TGAAAGACCA GCTACTGGCA GCCAAACATA TCCAATCAAA GGCCATTGAG 
GTTCGTCACG CTTCCTTGAT GAAACGCTGG AATCAGCTGC TTGCTAATTC TGCAGCCAGG 
AAAAAGAAAC TCTTGGAGGC TCAGGAGCAC TTCAGAAAGG TTGAAGATCT CTTCTTGACT 
TTTGCCAAGA AGGCCTCTGC CTTCAACAGT TGGTTTGAGA ATGCTGAGGA GGACCTGACG 
GATCCCGTGC GCTGCAATTC CCTGGAAGAA ATCAAAGCAC TGCGAGAAGC CCACGATGCC 
TTCCGTTCCT CACTTAGCTC TGCCCAAGCT GACTTCAACC AGCTGGCAGA GCTTGATCGG 
CAGATCAAGA GCTTCCGTGT AGCCTCCAAC CCGTACACTT GGTTTACTAT GGAGGCTCTT 
GAAGAAACTT GGAGGAATCT GCAGAAAATT ATCAAGGAGC GTGAATTGGA GTTGCAGAAG 
GAGCAGCGGA GGCAGGAAGA AAATGACAAG TTGCGCCAGG AGTTTGCTCA GCATGCTAAT 
GCCTTCCACC AGTGGATTCA GGAGACAAGG ACTTACCTGC TAGATGGGTC CTGTATGGTG 
GAGGAGTCGG GAACACTGGA ATCCCAGCTG GAAGCTACTA AACGCAAGCA CCAGGAGATC 
CGGGCTATGA GGAGCCAGCT GAAGAAGATT GAGGACCTTG GAGCAGCCAT GGAAGAGGCA 
CTTATCTTGG ACAACAAATA CACAGAGCAC AGCACCGTGG GGCTGGCGCA GCAGTGGGAC 
CAGCTTGACC AGCTGGGAAT GAGAATGCAG CACAATCTGG AACAGCAGAT TCAAGCTCGA 
AACACAACTG GAGTCACAGA GGAGGCCCTG AAGGAGTTCA GCATGATGTT CAAGCACTTT 
GACAAGGACA AATCTGGACG TCTTAATCAC CAGGAGTTTA AGTCTTGCTT GCGCTCCCTT 
GGCTATGACC TGCCCATGGT TGAGGAAGGA GAGCCTGACC CCGAATTTGA GTCTATTCTT 
GACACTGTTG ATCCCAACAG GGATGGCCAC GTCTCGCTGC AGGAGTACAT GGCGTTCATG 
ATCAGCAGGG AAACAGAGAA CGTGAAATCC AGCGAGGAGA TTGAGAGTGC TTTCCGTGCC 
CTCAGCTCGG AGAGGAAGCC TTACGTGACC AAGGAGGAGC TCTACCAGAA CCTGACCCGG 
GAGCAGGCCG ACTACTGCAT TTCTCACATG AAGCCCTACA TGGATGGCAA GGGCAGAGAA 
CTTCCTTCTG CCTACGACTA CATAGAATTT ACACGTTCAC TCTTTGTGAA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Non-equilibrium hydrogen exchange for determination of H-bond strength and water accessibility in solid proteins.
Journal of biomolecular NMR68(1)7-17(2017 May)
Grohe K,Movellan KT,Vasa SK,Giller K,Becker S,Linser R

Thermal stability of chicken brain ?-spectrin repeat 17: a spectroscopic study.
Journal of biomolecular NMR53(2)71-83(2012 Jun)
Brenner AK,Kieffer B,Trav? G,Fr?ystein NA,Raae AJ

Different members of a simple three-helix bundle protein family have very different folding rate constants and fold by different mechanisms.
Journal of molecular biology390(5)1074-85(2009 Jul)
Wensley BG,G?rtner M,Choo WX,Batey S,Clarke J

Distinguishing specific and nonspecific interdomain interactions in multidomain proteins.
Biophysical journal94(2)622-8(2008 Jan)
Randles LG,Batey S,Steward A,Clarke J

The high-resolution NMR structure of the R21A Spc-SH3:P41 complex: understanding the determinants of binding affinity by comparison with Abl-SH3.
BMC structural biology722(2007 Apr)
Casares S,Ab E,Eshuis H,Lopez-Mayorga O,van Nuland NA,Conejero-Lara F

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SPTAN1 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

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Target ORF information:

Target ORF information:

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ORF Insert Sequence:

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ORF Insert Sequence:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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ORF Insert Sequence:

Target ORF information:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence: