AgaP_AGAP006686 cDNA ORF clone, Anopheles gambiae str. PEST

Gene
Clones
gRNAs

Summary

Gene Symbol	AgaP_AGAP006686
Entrez Gene ID	1277275
Full Name	AGAP006686-PA
Synonyms	AgaP_ENSANGG00000002199,ENSANGG00000002199
Gene Type	protein-coding
Organism	Anopheles gambiae str. PEST

mRNA and Protein(s)

mRNA	Protein	Name
XM_316724.4	XP_316724.1	AGAP006686-PA

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Sptan1	NP_741984.2
Danio rerio (zebrafish)	spna2	NP_001091958.1
Caenorhabditis elegans (roundworm)	spc-1	NP_001257000.1
Xenopus tropicalis (tropical clawed frog)	sptan1	NP_001090674.1
Canis lupus familiaris (dog)	SPTAN1	XP_005625341.1
Bos taurus (cattle)	SPTAN1	NP_001107628.1
Mus musculus (house mouse)	Sptan1	NP_001171138.1
Gallus gallus (chicken)	SPTAN1	NP_001036003.1
Homo sapiens (human)	SPTAN1	NP_001123910.1
	SPTAN1	XP_002800011.1
Drosophila melanogaster (fruit fly)	alpha-Spec	NP_001261286.1
Pan troglodytes (chimpanzee)	SPTAN1	XP_003312345.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006686	XP_316724.1

Gene Ontology
Bibliography

Related articles in PubMed

The Anopheles gambiae genome: an update.
Mongin E, Louis C, Holt RA, Birney E, Collins FH
Trends in parasitology20(2)49-52(2004 Feb)

The genome sequence of the malaria mosquito Anopheles gambiae.
Holt RA, Subramanian GM, Halpern A, Sutton GG, Charlab R, Nusskern DR, Wincker P, Clark AG, Ribeiro JM, Wides R, Salzberg SL, Loftus B, Yandell M, Majoros WH, Rusch DB, Lai Z, Kraft CL, Abril JF, Anthouard V, Arensburger P, Atkinson PW, Baden H, de Berardinis V, Baldwin D, Benes V, Biedler J, Blass C, Bolanos R, Boscus D, Barnstead M, Cai S, Center A, Chaturverdi K, Christophides GK, Chrystal MA, Clamp M, Cravchik A, Curwen V, Dana A, Delcher A, Dew I, Evans CA, Flanigan M, Grundschober-Freimoser A, Friedli L, Gu Z, Guan P, Guigo R, Hillenmeyer ME, Hladun SL, Hogan JR, Hong YS, Hoover J, Jaillon O, Ke Z, Kodira C, Kokoza E, Koutsos A, Letunic I, Levitsky A, Liang Y, Lin JJ, Lobo NF, Lopez JR, Malek JA, McIntosh TC, Meister S, Miller J, Mobarry C, Mongin E, Murphy SD, O'Brochta DA, Pfannkoch C, Qi R, Regier MA, Remington K, Shao H, Sharakhova MV, Sitter CD, Shetty J, Smith TJ, Strong R, Sun J, Thomasova D, Ton LQ, Topalis P, Tu Z, Unger MF, Walenz B, Wang A, Wang J, Wang M, Wang X, Woodford KJ, Wortman JR, Wu M, Yao A, Zdobnov EM, Zhang H, Zhao Q, Zhao S, Zhu SC, Zhimulev I, Coluzzi M, della Torre A, Roth CW, Louis C, Kalush F, Mural RJ, Myers EW, Adams MD, Smith HO, Broder S, Gardner MJ, Fraser CM, Birney E, Bork P, Brey PT, Venter JC, Weissenbach J, Kafatos FC, Collins FH, Hoffman SL
Science (New York, N.Y.)298(5591)129-49(2002 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following AgaP_AGAP006686 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AgaP_AGAP006686 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAh05565	XM_316724.4 Latest version!	Anopheles gambiae str. PEST AGAP006686-RA (AgaP_AGAP006686), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AgaP_AGAP006686 ( XM_316724.4 ) cDNA ORF clone, Anopheles gambiae str. PEST -> XP_316724.1 Anopheles gambiae str. PEST AGAP006686-RA (AgaP_AGAP006686), partial mRNA.

Price & Availability↑

CloneID	OAh05565
Clone ID Related Accession (Same CDS sequence)	XM_316724.4
Accession Version	XM_316724.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7254bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-25
Organism	Anopheles gambiae str. PEST
Product	AGAP006686-PA
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NT_078265). On Oct 15, 2007 this sequence version replaced XM_316724.3. COMPLETENESS: incomplete on the 5' end.

ATGGAGCAGT TCACCCCGAA GGAGGTGCGC ATCCTCGAGA GCGCCGAAGA CATCCAGGAG 
CGCCGCGACC AGGTGCTGAA TCGCTACAGC GAGTTCAAGC TCGAGACGCG CCAGAAGCGC 
GAAAAGCTGG AAGACTCGCG CCGGTTCCAG TACTTCAAGC GCGATTCCGA CGAGCTGGAA 
AGCTGGATCA ACGAGAAGCT GCAGGCGGCG AGCGAGGAAA GCTACCGCGA CCCGACCAAC 
CTGCAGGCCA AGATCCAGAA GCACCAGGCG TTCGAGGCGG AGGTGTCCGC CCACAGCAAC 
GCGATCGTGG TGCTGGACAA CACCGGCCAG GAGATGATCA ACCAGGGCCA CTTTGCATCC 
GAGACGATCC AGCGCCGGCT GGAGGAGCTG CAGCGCCTGT GGGAGCTGCT GCTGTCGCGG 
CTGGCCGAGA AGGGCATGAA GCTGCAGCAG GCCCTGGTGC TGGTGCAGTT CCTGCGCCAC 
TGCGAGGAGG TGATGTTCTG GATCAAGGAC AAGGAAGCGT TCGTGACGGC GGACGAGTTC 
GGGCAGGATC TCGAGCACGT GGAGGTGCTG CAGCGCAAGT TCGACGAGTT CCAGAAGGAC 
ATGGCGTCGC AGGAGTACCG CGTGACGGAG GTGAACGAGC TGGCGGACAA GCTGCTGTTC 
GGCGGGCACC CGGAGCGGGA AACGATCACG CGCAAGAAGG AGGAACTGAA CGAGGCCTGG 
CAGCGCTTGA AGCAGCTGGC CATATTGCGC CAGGAGAAGC TGTTCGGGGC GCACGAAATC 
CAGCGTTTCA ACCGCGATGC GGATGAGACG GTTGCGTGGA TCGCTGAGAA GGATGTCGTG 
CTGTCGTCGG ACGATTACGG GCGCGATCTG GCCAGCGTGC AAGCGCTGCA GCGCAAGCAC 
GAAGGTGTGG AGCGTGATCT GGCGGCGCTC GAGGACAAGG TGGCCGCCCT CGGTACCGAG 
GCTGGCCGCC TGTGCAGCAT ACACGCCGAT CACAGCGAGC AGATTCGTGA GAAGCAGGCA 
GAGATTGCCG CGTACTGGCA GTCGCTGACC GCGAAGGCCA AGGAGCGCAA GCAGAAGCTC 
GACGAGTCCT ACTTCTTGCA CCGTTTCCTC GCCGACTTCC GTGATCTGGT GTCGTGGATC 
AACGGTATGA AGGCGATCAT CTCCGCGGAC GAGCTGGCGA AGGATGTGGC CGGTGCGGAG 
GCGCTGCTCG AGCGGCACCA GGAGCACAAG GGCGAGATCG ATGCTCGCGT GGACAGCTTC 
AAGCTGACGA CCGAGGCCGG CCGGCAGCTG CTCGAGCGGG AGCACTACGC CGCGGCGGAG 
GTGCAGGAGA AGCTGGCCGC GCTCGAGAAC GACAAGAGCT CGCTGCTGGT TTTGTGGGAG 
GATCGCCGCA TCCTGTACGA GCAGTGCATG GATCTGCAGC TGTTCTACCG CGACACCGAG 
CAGGCGGACA CGTGGATGGC GAAGCAGGAA GCGTTCCTGG CGAACGAGGA TCTGGGCGAC 
TCGCTCGACT CGGTCGAGGC GCTGATCAAG AAGCACGAGG ACTTTGAGAA GAGTCTGGCC 
GCGCAGGAGG AGAAGATAAA GGCGCTGGAC GTGTTTGCGA CGAAGCTGAT CGACGGGCAG 
CACTACGCGG CGGACGATGT GGCGCAACGT CGCGCCATGC TGCTGGCGCG CCGTTCCGCG 
CTGCAGGAGA AATCGTCCGT GCGCCAGAAG CTGCTGGAGG ACTCGAACGC GCTGCAGCAG 
TTCGAGCGCG ACTGCGACGA AACGAAGGGC TGGATCAGCG AGAAGCTGAA GTTCGCGACC 
GACGACAGCT ACCTCGACCC GACCAACCTG AACGGCAAGG TGCAGAAGCA CACCAACTTC 
GAGCACGAGC TGACGGCGAA CAAGAGCCGC ATCGAGGACA TCACGGCTAC CGGGCAGGAG 
CTGGCCACGA AGGAGCACTA CGCCGCGGAC AAGGTGAACG CACGGATGCA GGAAATCGTG 
ACGCTGTGGG AGTCGCTGGT GCGCGCCTCG GACAAGAAGG GCTGCAAGCT GCAGGAAGCC 
TCGCAGCAGC AGCAGTTCAA CCGCACCGTC GAGGACATCG AGCTGTGGCT GAGCGAGGTC 
GAGGGTCAGC TGCTGTCCGA GGACTACGGC AAGGATCTGA CCAGCGTGCA GAATCTGCAG 
AAGAAGCAGG CCCTGCTGGA GGCGGACGTG ATGGCGCACC AGGACCGTAT CGAGGGCATC 
AAGGTGGCGG CCAACAAGTT CGTCGAGAGC GGCCACTTCG ATGCGGACAA CATTCGCGCC 
AAGGAGGCGG CCCTGTCGAA GCGGTACGCG GCGCTGGCCG AACCGATGTC GATCCGCAAG 
CAGCGCCTGC TCGACTCGCT CCAGGTGCAG CAGCTGTTCC GCGATCTGGA GGACGAGGCG 
GCGTGGATCC GCGAGAAGGA ACCGGTCGCG GCGTCGACCA ACCGTGGCCG CGATCTGATC 
GGTGTGCAGA ACCTGATCAA GAAGCACCAG GCGGTGCTGG CGGAAATCAA CAACCACGAG 
AGCCGCTGCG CCGGTGTCAT CTCAAACGGG GAGCAGATGC TGAACGAGCA GCCAACCGCC 
AGCGAGGAGA TCAAGCTGCG GCTGGACGCG CTCAAGGATC AGTGGAACTC GCTGAAGGAG 
AAGTCGAACC AGCGCAAGCA GGATCTGGAA GATTCGCTGC AGGCCCACCA GTACTTTGCC 
GACGCGAACG AGGCCGAATC GTGGATGCGC GAGAAGGAAC CGATCGTCTC GAACCAGGAC 
TACGGCAAGG ATGAGGATTC GTCCGAGGCG CTGCTGAAGA AGCACGAGGC GCTCGTGTCC 
GACCTGGAAG CGTTCGGCAA CACGATCCAG GCGCTGCAGG AGCAGGCGAA GAACTGCCGC 
CAGCAGGAGA CGCCGGTCGT CGACATTACC GGCAAGGAGT GCGTGATGGC GCTGTATGAC 
TACACGGAAA AGTCGCCGCG CGAGGTGTCG ATGAAGAAGA ACGACGTGCT GACGCTGCTG 
AACTCGAACA ACAAGGACTG GTGGAAGGTG GAGGTGAACG ATCGCCAAGG CTTCGTGCCG 
GCGGCCTACA TCAAGAAGAT CGACCCAGGG CTAAGCGCCA GCCAGCAGAA CCTGATCGAT 
GGGCACTCGA TCGCGAAGCG CCAGACGCAG ATCAACAGCC AGTACGACAA TCTGCTCGCC 
CTGGCCCGGG AGCGCCAGAA CAAGCTGAAC GAAACGGTGA AGGCGTACGT GCTGGTGCGC 
GAGGCGGCCG ATCTCGCCGC GTGGATCAAG GACAAGGAGA GCCACGCGCA GATCAAGGAC 
GTCGGCGAAG ATCTCGAGGA GGTGGAGGTG CTGCAGAAGA AGTTCGACGA CTTCAACGAC 
GACCTGAAGG CGAACGAGGT TCGGCTGGCG AAGCTGAACG AGATCGCCAT CCAGCTGACG 
TCGCTCGGTC AGACGGAGGC CGCACTGAAG ATCAAGACGC AAATCCAAAC GCTCAACGAA 
GAGTGGGCCA CCCTGCAGAC GATCACGCAG GAGCGGGCCA GCCAGCTTGG GTCGGCGCAC 
GAGGTCCAAC GGTTCCACCG CGACGTGGAC GAAACGAAGG ACTGGATCGC GGAGAAGGAG 
AACGCGCTGA ACAACGACGA GCTGGGCAAG GATTTGCGCG GCGTGCAAAC GTTGCAGCGC 
AAGCACGAGG GGCTGGAGCG TGATTTGGCC GCGCTGCAGG ACAAGATCCG CCAGCTGGAC 
GAGACGGCCA ACCGGCTGAT GCAGTCGCAC CCGGACACGG CCGAGCAGAC GTACGCTAAG 
CAGAAGGAGA TCAACGAGGA GTGGCAGCAG GTGGTGACCA AGGCGCAGCA GCGCAAGGAG 
AAGCTGCTCG ACTCGTACGA TCTGCAGCGC TTCCTGAGCG ACTACCGCGA CCTGTCCGCC 
TGGATCAGCT CGATGATGGG GCTGGTGACG TCGGAAGAGC TCGCGAACGA TGTTACCGGC 
GCGGAAGCGT TGATCGAGCG TCATCAGGAA CATCGCACGG AGGTGGATGC ACGCGCGGGT 
ACGTTCTCGG CGTTCGAGCA GTTCGGCAAT GAGCTGCTGC AGGCGAACCA CTATGCGGCG 
CCCGAAATTC AGGAGAAGAT CGAAAACCTG AACAAGGCCC GGGAGGAGCT GGAGCGTGCG 
TGGACGGCGC GTCGCCTGCA GCTGGACCAA AATCTGGACC TGCAGCTGTA TCTGCGCGAT 
TGCGAGCAGG CCGAAAACTG GATGAGCGCC CGCGAAGCGT TCCTGAACGC GGAGGAGGTC 
GACTCGAAGG GTGACAACGT GGAGGCCCTG ATCAAGAAGC ACGAGGACTT CGATAAGGCG 
ATCAACGGCC ATGAGGAGAA GATCGGCGCG TTGCAGGTGC TGGCCGATCA GCTGATCGCG 
CAGGAGCATT ACGCGGGCCG TTTGATCGAT GCCAAGCGGC AGGAGGTGCT GGATCGCTGG 
CGCCATCTGA AGGAGGATCT GATCGAGAAA CGCTCGCGGC TCGGCGACGA GCAGACGCTG 
CAGCAGTTCT CGCGCGATGC CGACGAGATC GAGAACTGGA TCGCGGAGAA GCTGCAGCTG 
GCGACGGAGG AAAGCTACAA GGATCCGGCC AACATTCAGT CGAAGCACCA GAAGCACCAG 
GCGTTCGAAG CGGAGCTGGC GGCGAACGCG GACCGCATCT CGAGCGTGCT GGCGATGGGT 
AGCAACCTGA TCGATCGCAA CCAGTGCAGC GGTTCGGAGG AGGCGGTGCA GAAGCGCCTG 
ACGCAGATCG CCGACCAGTG GGAGTACCTG ACGCAGAAGA CGACCGAGAA GTCGCTGAAG 
CTGAAGGAGG CGAACAAGCA GCGCACGTAC ATCGCGGCCG TGAAGGATCT GGACTTCTGG 
CTGGGCGAGG TGGAGAGTCT GCTGACGTCG GAGGACGCCG GCAAGGATCT GGCCTCCGTG 
CAGAACCTGA TGAAGAAGCA TCAGCTGGTC GAGGCGGACA TTCACGCGCA CGAGGATCGC 
ATCAAGGACA TGAACAGCCA GGCGGACTCG CTGGTGGAGA GTGGCCAGTT CGACAGTGCC 
GGCATCCAGG AGAAGCGCCA GTCGATCAAC GAACGGTACG AGCGCATCCG CAACCTGGCT 
GCCCATCGGC AGGCTCGGCT GAACGAGGCG AACACGCTGC ACCAGTTCTT CCGCGACATC 
GCGGACGAGG AGAGCTGGAT CAAGGAGAAG AAGCTGCTGG TCGGATCGGA CGACTACGGT 
CGCGATCTGA CCGGTGTGCA GAATCTGAAG AAGAAGCACA AGCGGCTCGA GGCCGAGCTT 
GCCTCGCACG AACCCGCGAT CCAGGCGGTG CAGGAGGCGG GCGAGAAGCT GATGGACGTT 
TCGAACCTTG GGGTGCCGGA GATTGAGCAG CGCCTGAAGG CACTGAACCA GGCGTGGACC 
GAGCTGAAGG GACTGGCGGC CACTCGCGGC CAGAAGCTGG ACGAGTCGCT CATCTACCAG 
CAGTTCCTGG CGAAGGTCGA GGAGGAGGAG GCCTGGATTA CGGAGAAGCA GCAGCTGCTG 
TCGGTCGAGG ACTACGGTGA CTCGATGGCG GCTGTCCAGG GTCTGATGAA GAAGCACGAC 
GCGTTCGAGA CGGACTTTGC GGTCCATCGC GATCGCTGCT CGGACATCCG CGACCATGGC 
CAGACGCTGG TAACGAACAA CAACCACCAT GGCGATAGCA TCTCGCAGCG CTGCGTCCAG 
CTGGACAAGA AGCTGGAGAA CCTGCAGGCC CTGGCGACGC GCCGCAAGAC GGCGCTGATG 
GACAACTTTG CCTACCTGCA GTTCATGTGG AAGGCGGACG TCGTGGAGAG CTGGATCGCG 
GACAAGGAGA ATCACGTCAA GTCGGAAGAG TTCGGCCGTG ATCTGTCCAC CGTTCAAACG 
TTGCTGACGA AGCAGGAGAC ATTCGATGCT GGTCTCTCGG CGTTTGAGCA TGAGGGTATC 
CACAACATTA CCGCCCTCAA GGATCAGCTG ATCAACGCGA ACCACGCCCA GTCGGCCGCC 
ATTCTCAAGC GCCACGAGGA CGTGCTGACG CGCTGGCAGA AGCTGCGCGC AGATTCGGAG 
GCGCGCAAGT ACCGCCTGCT GAACATGCAG GACCAGTTCC GCCAGATCGA GGATCTGTAT 
CTGACGTTCG CCAAGAAGGC GTCGGCATTC AACTCGTGGT TCGAGAACGC GGAGGAAGAT 
CTGACCGATC CGGTGCGCTG CAACTCGATC GAGGAGATCA AGGCGCTGCG GGAGGCACAT 
GCCGCGTTCC AGGCATCGCT GTCGTCGGCC CAGGTCGACT TCCAGGCGCT GGCCGCGCTC 
GACCAGAAGA TCAAGAGCTT CAACGTCGGC CCGAACCCGT ACACCTGGTT CACGATGGAG 
GCGCTGGAGG ATACGTGGCG CAATCTGCAG AAGATCATCG AGGAGCGCGA TGCCGAGCTG 
GCGAAGGAGG TCCATCGGCA GGAGGAGAAC GACAAGCTGC GCAAGGAGTT TGCCAAGCAC 
GCCAACCTGT TCCACCAGTG GCTGACGGAG ACAAGAACGT CGCTCATGGA TGGTTCGGGA 
TCGCTGGAGG AACAGTTCGA GGCGCTCTGC CACAAGGCGA ACGAGATCCG CGCACGCCGT 
GGCGATCTGA AGAAGATCGA GGAGCTCGGC GCCACGCTGG AGGAGCATCT GATTCTGGAC 
AACCGCTACA CGGAGCACTC GACGGTCGGG TTGGCCCAGC AGTGGGACCA GCTCGACCAG 
CTGGCCATGC GCATGCAGCA CAACCTGAAG CAGCAGATCC AGGCCCGCAA CCAGTCCGGC 
GTGTCGGAAG ACTCGCTGAA GGAATTCTCG ATGATGTTCA AGCACTTCGA CAAGGACAAG 
AGCGGCAAGC TGAACCACCA GGAGTTCAAA TCGTGTCTGC GTGCCCTCGG CTACGACCTG 
CCCATGGTGG AGGAAGGCCA ACCGGACCCG GAGTTCGAGG AAATTTTGAA CGTGGTCGAT 
CCGAACCGGG ACGGCCAGGT GTCGCTGCAG GAGTACATCG CGTTCATGAT TTCGAAGGAA 
ACGGAGAACG TGCAAAGCTT CGAGGAGATC GAGAATGCTT TCCGCGCCAT CACCGCCTCC 
GATCCGCGTC CCCGCCCATA TGTCACCAAG GAGGAACTCT ATTCCAACCT CACCAAAGAC 
ATGGCGGATT ACTGCGTGCA GCGCATGAAA CCGTACAACG ACCCGAAAAC GGGCCACCCC 
ATTACGGGTG CCCTGGATTA CGTGGAGTTT ACCAGAACCT TGTTCCAAAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_316724.1
CDS	1..7254
Translation	MEQFTPKEVRILESAEDIQERRDQVLNRYSEFKLETRQKREKLEDSRRFQYFKRDSDELESWINEKLQAASEESYRDPTNLQAKIQKHQAFEAEVSAHSNAIVVLDNTGQEMINQGHFASETIQRRLEELQRLWELLLSRLAEKGMKLQQALVLVQFLRHCEEVMFWIKDKEAFVTADEFGQDLEHVEVLQRKFDEFQKDMASQEYRVTEVNELADKLLFGGHPERETITRKKEELNEAWQRLKQLAILRQEKLFGAHEIQRFNRDADETVAWIAEKDVVLSSDDYGRDLASVQALQRKHEGVERDLAALEDKVAALGTEAGRLCSIHADHSEQIREKQAEIAAYWQSLTAKAKERKQKLDESYFLHRFLADFRDLVSWINGMKAIISADELAKDVAGAEALLERHQEHKGEIDARVDSFKLTTEAGRQLLEREHYAAAEVQEKLAALENDKSSLLVLWEDRRILYEQCMDLQLFYRDTEQADTWMAKQEAFLANEDLGDSLDSVEALIKKHEDFEKSLAAQEEKIKALDVFATKLIDGQHYAADDVAQRRAMLLARRSALQEKSSVRQKLLEDSNALQQFERDCDETKGWISEKLKFATDDSYLDPTNLNGKVQKHTNFEHELTANKSRIEDITATGQELATKEHYAADKVNARMQEIVTLWESLVRASDKKGCKLQEASQQQQFNRTVEDIELWLSEVEGQLLSEDYGKDLTSVQNLQKKQALLEADVMAHQDRIEGIKVAANKFVESGHFDADNIRAKEAALSKRYAALAEPMSIRKQRLLDSLQVQQLFRDLEDEAAWIREKEPVAASTNRGRDLIGVQNLIKKHQAVLAEINNHESRCAGVISNGEQMLNEQPTASEEIKLRLDALKDQWNSLKEKSNQRKQDLEDSLQAHQYFADANEAESWMREKEPIVSNQDYGKDEDSSEALLKKHEALVSDLEAFGNTIQALQEQAKNCRQQETPVVDITGKECVMALYDYTEKSPREVSMKKNDVLTLLNSNNKDWWKVEVNDRQGFVPAAYIKKIDPGLSASQQNLIDGHSIAKRQTQINSQYDNLLALARERQNKLNETVKAYVLVREAADLAAWIKDKESHAQIKDVGEDLEEVEVLQKKFDDFNDDLKANEVRLAKLNEIAIQLTSLGQTEAALKIKTQIQTLNEEWATLQTITQERASQLGSAHEVQRFHRDVDETKDWIAEKENALNNDELGKDLRGVQTLQRKHEGLERDLAALQDKIRQLDETANRLMQSHPDTAEQTYAKQKEINEEWQQVVTKAQQRKEKLLDSYDLQRFLSDYRDLSAWISSMMGLVTSEELANDVTGAEALIERHQEHRTEVDARAGTFSAFEQFGNELLQANHYAAPEIQEKIENLNKAREELERAWTARRLQLDQNLDLQLYLRDCEQAENWMSAREAFLNAEEVDSKGDNVEALIKKHEDFDKAINGHEEKIGALQVLADQLIAQEHYAGRLIDAKRQEVLDRWRHLKEDLIEKRSRLGDEQTLQQFSRDADEIENWIAEKLQLATEESYKDPANIQSKHQKHQAFEAELAANADRISSVLAMGSNLIDRNQCSGSEEAVQKRLTQIADQWEYLTQKTTEKSLKLKEANKQRTYIAAVKDLDFWLGEVESLLTSEDAGKDLASVQNLMKKHQLVEADIHAHEDRIKDMNSQADSLVESGQFDSAGIQEKRQSINERYERIRNLAAHRQARLNEANTLHQFFRDIADEESWIKEKKLLVGSDDYGRDLTGVQNLKKKHKRLEAELASHEPAIQAVQEAGEKLMDVSNLGVPEIEQRLKALNQAWTELKGLAATRGQKLDESLIYQQFLAKVEEEEAWITEKQQLLSVEDYGDSMAAVQGLMKKHDAFETDFAVHRDRCSDIRDHGQTLVTNNNHHGDSISQRCVQLDKKLENLQALATRRKTALMDNFAYLQFMWKADVVESWIADKENHVKSEEFGRDLSTVQTLLTKQETFDAGLSAFEHEGIHNITALKDQLINANHAQSAAILKRHEDVLTRWQKLRADSEARKYRLLNMQDQFRQIEDLYLTFAKKASAFNSWFENAEEDLTDPVRCNSIEEIKALREAHAAFQASLSSAQVDFQALAALDQKIKSFNVGPNPYTWFTMEALEDTWRNLQKIIEERDAELAKEVHRQEENDKLRKEFAKHANLFHQWLTETRTSLMDGSGSLEEQFEALCHKANEIRARRGDLKKIEELGATLEEHLILDNRYTEHSTVGLAQQWDQLDQLAMRMQHNLKQQIQARNQSGVSEDSLKEFSMMFKHFDKDKSGKLNHQEFKSCLRALGYDLPMVEEGQPDPEFEEILNVVDPNRDGQVSLQEYIAFMISKETENVQSFEEIENAFRAITASDPRPRPYVTKEELYSNLTKDMADYCVQRMKPYNDPKTGHPITGALDYVEFTRTLFQN

Target ORF information:

RefSeq Version	XM_316724.4
Organism	Anopheles gambiae str. PEST
Definition	Anopheles gambiae str. PEST AGAP006686-RA (AgaP_AGAP006686), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_316724.4

ORF Insert Sequence:

ATGGAGCAGT TCACCCCGAA GGAGGTGCGC ATCCTCGAGA GCGCCGAAGA CATCCAGGAG 
CGCCGCGACC AGGTGCTGAA TCGCTACAGC GAGTTCAAGC TCGAGACGCG CCAGAAGCGC 
GAAAAGCTGG AAGACTCGCG CCGGTTCCAG TACTTCAAGC GCGATTCCGA CGAGCTGGAA 
AGCTGGATCA ACGAGAAGCT GCAGGCGGCG AGCGAGGAAA GCTACCGCGA CCCGACCAAC 
CTGCAGGCCA AGATCCAGAA GCACCAGGCG TTCGAGGCGG AGGTGTCCGC CCACAGCAAC 
GCGATCGTGG TGCTGGACAA CACCGGCCAG GAGATGATCA ACCAGGGCCA CTTTGCATCC 
GAGACGATCC AGCGCCGGCT GGAGGAGCTG CAGCGCCTGT GGGAGCTGCT GCTGTCGCGG 
CTGGCCGAGA AGGGCATGAA GCTGCAGCAG GCCCTGGTGC TGGTGCAGTT CCTGCGCCAC 
TGCGAGGAGG TGATGTTCTG GATCAAGGAC AAGGAAGCGT TCGTGACGGC GGACGAGTTC 
GGGCAGGATC TCGAGCACGT GGAGGTGCTG CAGCGCAAGT TCGACGAGTT CCAGAAGGAC 
ATGGCGTCGC AGGAGTACCG CGTGACGGAG GTGAACGAGC TGGCGGACAA GCTGCTGTTC 
GGCGGGCACC CGGAGCGGGA AACGATCACG CGCAAGAAGG AGGAACTGAA CGAGGCCTGG 
CAGCGCTTGA AGCAGCTGGC CATATTGCGC CAGGAGAAGC TGTTCGGGGC GCACGAAATC 
CAGCGTTTCA ACCGCGATGC GGATGAGACG GTTGCGTGGA TCGCTGAGAA GGATGTCGTG 
CTGTCGTCGG ACGATTACGG GCGCGATCTG GCCAGCGTGC AAGCGCTGCA GCGCAAGCAC 
GAAGGTGTGG AGCGTGATCT GGCGGCGCTC GAGGACAAGG TGGCCGCCCT CGGTACCGAG 
GCTGGCCGCC TGTGCAGCAT ACACGCCGAT CACAGCGAGC AGATTCGTGA GAAGCAGGCA 
GAGATTGCCG CGTACTGGCA GTCGCTGACC GCGAAGGCCA AGGAGCGCAA GCAGAAGCTC 
GACGAGTCCT ACTTCTTGCA CCGTTTCCTC GCCGACTTCC GTGATCTGGT GTCGTGGATC 
AACGGTATGA AGGCGATCAT CTCCGCGGAC GAGCTGGCGA AGGATGTGGC CGGTGCGGAG 
GCGCTGCTCG AGCGGCACCA GGAGCACAAG GGCGAGATCG ATGCTCGCGT GGACAGCTTC 
AAGCTGACGA CCGAGGCCGG CCGGCAGCTG CTCGAGCGGG AGCACTACGC CGCGGCGGAG 
GTGCAGGAGA AGCTGGCCGC GCTCGAGAAC GACAAGAGCT CGCTGCTGGT TTTGTGGGAG 
GATCGCCGCA TCCTGTACGA GCAGTGCATG GATCTGCAGC TGTTCTACCG CGACACCGAG 
CAGGCGGACA CGTGGATGGC GAAGCAGGAA GCGTTCCTGG CGAACGAGGA TCTGGGCGAC 
TCGCTCGACT CGGTCGAGGC GCTGATCAAG AAGCACGAGG ACTTTGAGAA GAGTCTGGCC 
GCGCAGGAGG AGAAGATAAA GGCGCTGGAC GTGTTTGCGA CGAAGCTGAT CGACGGGCAG 
CACTACGCGG CGGACGATGT GGCGCAACGT CGCGCCATGC TGCTGGCGCG CCGTTCCGCG 
CTGCAGGAGA AATCGTCCGT GCGCCAGAAG CTGCTGGAGG ACTCGAACGC GCTGCAGCAG 
TTCGAGCGCG ACTGCGACGA AACGAAGGGC TGGATCAGCG AGAAGCTGAA GTTCGCGACC 
GACGACAGCT ACCTCGACCC GACCAACCTG AACGGCAAGG TGCAGAAGCA CACCAACTTC 
GAGCACGAGC TGACGGCGAA CAAGAGCCGC ATCGAGGACA TCACGGCTAC CGGGCAGGAG 
CTGGCCACGA AGGAGCACTA CGCCGCGGAC AAGGTGAACG CACGGATGCA GGAAATCGTG 
ACGCTGTGGG AGTCGCTGGT GCGCGCCTCG GACAAGAAGG GCTGCAAGCT GCAGGAAGCC 
TCGCAGCAGC AGCAGTTCAA CCGCACCGTC GAGGACATCG AGCTGTGGCT GAGCGAGGTC 
GAGGGTCAGC TGCTGTCCGA GGACTACGGC AAGGATCTGA CCAGCGTGCA GAATCTGCAG 
AAGAAGCAGG CCCTGCTGGA GGCGGACGTG ATGGCGCACC AGGACCGTAT CGAGGGCATC 
AAGGTGGCGG CCAACAAGTT CGTCGAGAGC GGCCACTTCG ATGCGGACAA CATTCGCGCC 
AAGGAGGCGG CCCTGTCGAA GCGGTACGCG GCGCTGGCCG AACCGATGTC GATCCGCAAG 
CAGCGCCTGC TCGACTCGCT CCAGGTGCAG CAGCTGTTCC GCGATCTGGA GGACGAGGCG 
GCGTGGATCC GCGAGAAGGA ACCGGTCGCG GCGTCGACCA ACCGTGGCCG CGATCTGATC 
GGTGTGCAGA ACCTGATCAA GAAGCACCAG GCGGTGCTGG CGGAAATCAA CAACCACGAG 
AGCCGCTGCG CCGGTGTCAT CTCAAACGGG GAGCAGATGC TGAACGAGCA GCCAACCGCC 
AGCGAGGAGA TCAAGCTGCG GCTGGACGCG CTCAAGGATC AGTGGAACTC GCTGAAGGAG 
AAGTCGAACC AGCGCAAGCA GGATCTGGAA GATTCGCTGC AGGCCCACCA GTACTTTGCC 
GACGCGAACG AGGCCGAATC GTGGATGCGC GAGAAGGAAC CGATCGTCTC GAACCAGGAC 
TACGGCAAGG ATGAGGATTC GTCCGAGGCG CTGCTGAAGA AGCACGAGGC GCTCGTGTCC 
GACCTGGAAG CGTTCGGCAA CACGATCCAG GCGCTGCAGG AGCAGGCGAA GAACTGCCGC 
CAGCAGGAGA CGCCGGTCGT CGACATTACC GGCAAGGAGT GCGTGATGGC GCTGTATGAC 
TACACGGAAA AGTCGCCGCG CGAGGTGTCG ATGAAGAAGA ACGACGTGCT GACGCTGCTG 
AACTCGAACA ACAAGGACTG GTGGAAGGTG GAGGTGAACG ATCGCCAAGG CTTCGTGCCG 
GCGGCCTACA TCAAGAAGAT CGACCCAGGG CTAAGCGCCA GCCAGCAGAA CCTGATCGAT 
GGGCACTCGA TCGCGAAGCG CCAGACGCAG ATCAACAGCC AGTACGACAA TCTGCTCGCC 
CTGGCCCGGG AGCGCCAGAA CAAGCTGAAC GAAACGGTGA AGGCGTACGT GCTGGTGCGC 
GAGGCGGCCG ATCTCGCCGC GTGGATCAAG GACAAGGAGA GCCACGCGCA GATCAAGGAC 
GTCGGCGAAG ATCTCGAGGA GGTGGAGGTG CTGCAGAAGA AGTTCGACGA CTTCAACGAC 
GACCTGAAGG CGAACGAGGT TCGGCTGGCG AAGCTGAACG AGATCGCCAT CCAGCTGACG 
TCGCTCGGTC AGACGGAGGC CGCACTGAAG ATCAAGACGC AAATCCAAAC GCTCAACGAA 
GAGTGGGCCA CCCTGCAGAC GATCACGCAG GAGCGGGCCA GCCAGCTTGG GTCGGCGCAC 
GAGGTCCAAC GGTTCCACCG CGACGTGGAC GAAACGAAGG ACTGGATCGC GGAGAAGGAG 
AACGCGCTGA ACAACGACGA GCTGGGCAAG GATTTGCGCG GCGTGCAAAC GTTGCAGCGC 
AAGCACGAGG GGCTGGAGCG TGATTTGGCC GCGCTGCAGG ACAAGATCCG CCAGCTGGAC 
GAGACGGCCA ACCGGCTGAT GCAGTCGCAC CCGGACACGG CCGAGCAGAC GTACGCTAAG 
CAGAAGGAGA TCAACGAGGA GTGGCAGCAG GTGGTGACCA AGGCGCAGCA GCGCAAGGAG 
AAGCTGCTCG ACTCGTACGA TCTGCAGCGC TTCCTGAGCG ACTACCGCGA CCTGTCCGCC 
TGGATCAGCT CGATGATGGG GCTGGTGACG TCGGAAGAGC TCGCGAACGA TGTTACCGGC 
GCGGAAGCGT TGATCGAGCG TCATCAGGAA CATCGCACGG AGGTGGATGC ACGCGCGGGT 
ACGTTCTCGG CGTTCGAGCA GTTCGGCAAT GAGCTGCTGC AGGCGAACCA CTATGCGGCG 
CCCGAAATTC AGGAGAAGAT CGAAAACCTG AACAAGGCCC GGGAGGAGCT GGAGCGTGCG 
TGGACGGCGC GTCGCCTGCA GCTGGACCAA AATCTGGACC TGCAGCTGTA TCTGCGCGAT 
TGCGAGCAGG CCGAAAACTG GATGAGCGCC CGCGAAGCGT TCCTGAACGC GGAGGAGGTC 
GACTCGAAGG GTGACAACGT GGAGGCCCTG ATCAAGAAGC ACGAGGACTT CGATAAGGCG 
ATCAACGGCC ATGAGGAGAA GATCGGCGCG TTGCAGGTGC TGGCCGATCA GCTGATCGCG 
CAGGAGCATT ACGCGGGCCG TTTGATCGAT GCCAAGCGGC AGGAGGTGCT GGATCGCTGG 
CGCCATCTGA AGGAGGATCT GATCGAGAAA CGCTCGCGGC TCGGCGACGA GCAGACGCTG 
CAGCAGTTCT CGCGCGATGC CGACGAGATC GAGAACTGGA TCGCGGAGAA GCTGCAGCTG 
GCGACGGAGG AAAGCTACAA GGATCCGGCC AACATTCAGT CGAAGCACCA GAAGCACCAG 
GCGTTCGAAG CGGAGCTGGC GGCGAACGCG GACCGCATCT CGAGCGTGCT GGCGATGGGT 
AGCAACCTGA TCGATCGCAA CCAGTGCAGC GGTTCGGAGG AGGCGGTGCA GAAGCGCCTG 
ACGCAGATCG CCGACCAGTG GGAGTACCTG ACGCAGAAGA CGACCGAGAA GTCGCTGAAG 
CTGAAGGAGG CGAACAAGCA GCGCACGTAC ATCGCGGCCG TGAAGGATCT GGACTTCTGG 
CTGGGCGAGG TGGAGAGTCT GCTGACGTCG GAGGACGCCG GCAAGGATCT GGCCTCCGTG 
CAGAACCTGA TGAAGAAGCA TCAGCTGGTC GAGGCGGACA TTCACGCGCA CGAGGATCGC 
ATCAAGGACA TGAACAGCCA GGCGGACTCG CTGGTGGAGA GTGGCCAGTT CGACAGTGCC 
GGCATCCAGG AGAAGCGCCA GTCGATCAAC GAACGGTACG AGCGCATCCG CAACCTGGCT 
GCCCATCGGC AGGCTCGGCT GAACGAGGCG AACACGCTGC ACCAGTTCTT CCGCGACATC 
GCGGACGAGG AGAGCTGGAT CAAGGAGAAG AAGCTGCTGG TCGGATCGGA CGACTACGGT 
CGCGATCTGA CCGGTGTGCA GAATCTGAAG AAGAAGCACA AGCGGCTCGA GGCCGAGCTT 
GCCTCGCACG AACCCGCGAT CCAGGCGGTG CAGGAGGCGG GCGAGAAGCT GATGGACGTT 
TCGAACCTTG GGGTGCCGGA GATTGAGCAG CGCCTGAAGG CACTGAACCA GGCGTGGACC 
GAGCTGAAGG GACTGGCGGC CACTCGCGGC CAGAAGCTGG ACGAGTCGCT CATCTACCAG 
CAGTTCCTGG CGAAGGTCGA GGAGGAGGAG GCCTGGATTA CGGAGAAGCA GCAGCTGCTG 
TCGGTCGAGG ACTACGGTGA CTCGATGGCG GCTGTCCAGG GTCTGATGAA GAAGCACGAC 
GCGTTCGAGA CGGACTTTGC GGTCCATCGC GATCGCTGCT CGGACATCCG CGACCATGGC 
CAGACGCTGG TAACGAACAA CAACCACCAT GGCGATAGCA TCTCGCAGCG CTGCGTCCAG 
CTGGACAAGA AGCTGGAGAA CCTGCAGGCC CTGGCGACGC GCCGCAAGAC GGCGCTGATG 
GACAACTTTG CCTACCTGCA GTTCATGTGG AAGGCGGACG TCGTGGAGAG CTGGATCGCG 
GACAAGGAGA ATCACGTCAA GTCGGAAGAG TTCGGCCGTG ATCTGTCCAC CGTTCAAACG 
TTGCTGACGA AGCAGGAGAC ATTCGATGCT GGTCTCTCGG CGTTTGAGCA TGAGGGTATC 
CACAACATTA CCGCCCTCAA GGATCAGCTG ATCAACGCGA ACCACGCCCA GTCGGCCGCC 
ATTCTCAAGC GCCACGAGGA CGTGCTGACG CGCTGGCAGA AGCTGCGCGC AGATTCGGAG 
GCGCGCAAGT ACCGCCTGCT GAACATGCAG GACCAGTTCC GCCAGATCGA GGATCTGTAT 
CTGACGTTCG CCAAGAAGGC GTCGGCATTC AACTCGTGGT TCGAGAACGC GGAGGAAGAT 
CTGACCGATC CGGTGCGCTG CAACTCGATC GAGGAGATCA AGGCGCTGCG GGAGGCACAT 
GCCGCGTTCC AGGCATCGCT GTCGTCGGCC CAGGTCGACT TCCAGGCGCT GGCCGCGCTC 
GACCAGAAGA TCAAGAGCTT CAACGTCGGC CCGAACCCGT ACACCTGGTT CACGATGGAG 
GCGCTGGAGG ATACGTGGCG CAATCTGCAG AAGATCATCG AGGAGCGCGA TGCCGAGCTG 
GCGAAGGAGG TCCATCGGCA GGAGGAGAAC GACAAGCTGC GCAAGGAGTT TGCCAAGCAC 
GCCAACCTGT TCCACCAGTG GCTGACGGAG ACAAGAACGT CGCTCATGGA TGGTTCGGGA 
TCGCTGGAGG AACAGTTCGA GGCGCTCTGC CACAAGGCGA ACGAGATCCG CGCACGCCGT 
GGCGATCTGA AGAAGATCGA GGAGCTCGGC GCCACGCTGG AGGAGCATCT GATTCTGGAC 
AACCGCTACA CGGAGCACTC GACGGTCGGG TTGGCCCAGC AGTGGGACCA GCTCGACCAG 
CTGGCCATGC GCATGCAGCA CAACCTGAAG CAGCAGATCC AGGCCCGCAA CCAGTCCGGC 
GTGTCGGAAG ACTCGCTGAA GGAATTCTCG ATGATGTTCA AGCACTTCGA CAAGGACAAG 
AGCGGCAAGC TGAACCACCA GGAGTTCAAA TCGTGTCTGC GTGCCCTCGG CTACGACCTG 
CCCATGGTGG AGGAAGGCCA ACCGGACCCG GAGTTCGAGG AAATTTTGAA CGTGGTCGAT 
CCGAACCGGG ACGGCCAGGT GTCGCTGCAG GAGTACATCG CGTTCATGAT TTCGAAGGAA 
ACGGAGAACG TGCAAAGCTT CGAGGAGATC GAGAATGCTT TCCGCGCCAT CACCGCCTCC 
GATCCGCGTC CCCGCCCATA TGTCACCAAG GAGGAACTCT ATTCCAACCT CACCAAAGAC 
ATGGCGGATT ACTGCGTGCA GCGCATGAAA CCGTACAACG ACCCGAAAAC GGGCCACCCC 
ATTACGGGTG CCCTGGATTA CGTGGAGTTT ACCAGAACCT TGTTCCAAAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Anopheles gambiae genome: an update.
Trends in parasitology20(2)49-52(2004 Feb)
Mongin E,Louis C,Holt RA,Birney E,Collins FH

The genome sequence of the malaria mosquito Anopheles gambiae.
Science (New York, N.Y.)298(5591)129-49(2002 Oct)
Holt RA,Subramanian GM,Halpern A,Sutton GG,Charlab R,Nusskern DR,Wincker P,Clark AG,Ribeiro JM,Wides R,Salzberg SL,Loftus B,Yandell M,Majoros WH,Rusch DB,Lai Z,Kraft CL,Abril JF,Anthouard V,Arensburger P,Atkinson PW,Baden H,de Berardinis V,Baldwin D,Benes V,Biedler J,Blass C,Bolanos R,Boscus D,Barnstead M,Cai S,Center A,Chaturverdi K,Christophides GK,Chrystal MA,Clamp M,Cravchik A,Curwen V,Dana A,Delcher A,Dew I,Evans CA,Flanigan M,Grundschober-Freimoser A,Friedli L,Gu Z,Guan P,Guigo R,Hillenmeyer ME,Hladun SL,Hogan JR,Hong YS,Hoover J,Jaillon O,Ke Z,Kodira C,Kokoza E,Koutsos A,Letunic I,Levitsky A,Liang Y,Lin JJ,Lobo NF,Lopez JR,Malek JA,McIntosh TC,Meister S,Miller J,Mobarry C,Mongin E,Murphy SD,O'Brochta DA,Pfannkoch C,Qi R,Regier MA,Remington K,Shao H,Sharakhova MV,Sitter CD,Shetty J,Smith TJ,Strong R,Sun J,Thomasova D,Ton LQ,Topalis P,Tu Z,Unger MF,Walenz B,Wang A,Wang J,Wang M,Wang X,Woodford KJ,Wortman JR,Wu M,Yao A,Zdobnov EM,Zhang H,Zhao Q,Zhao S,Zhu SC,Zhimulev I,Coluzzi M,della Torre A,Roth CW,Louis C,Kalush F,Mural RJ,Myers EW,Adams MD,Smith HO,Broder S,Gardner MJ,Fraser CM,Birney E,Bork P,Brey PT,Venter JC,Weissenbach J,Kafatos FC,Collins FH,Hoffman SL

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AgaP_AGAP006686 cDNA ORF clone, Anopheles gambiae str. PEST

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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