Sptan1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sptan1
Entrez Gene ID	64159
Full Name	spectrin, alpha, non-erythrocytic 1
Synonyms	A2a,IPF,Spna2
General protein information	Preferred Names spectrin, alpha, non-erythrocytic 1 Names spectrin alpha chain, non-erythrocytic 1 a--fodrin alpha II spectrin alpha-fodrin alpha-spectrin 2 brain alpha-spectrin fodrin alpha chain inhibitory protein factor noerythroid alpha-spectrin 2 nonerythroid alpha-spectrin 2 spectrin alpha chain, brain spectrin, non-erythroid alpha chain
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3p12
Summary	a filamentous protein that plays an important role in membrane organization [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008761673.2	XP_008759895.1	spectrin alpha chain, non-erythrocytic 1 isoform X1
XM_008761675.2	XP_008759897.1	spectrin alpha chain, non-erythrocytic 1 isoform X3
XM_008761674.2	XP_008759896.1	spectrin alpha chain, non-erythrocytic 1 isoform X2
XM_008761676.2	XP_008759898.1	spectrin alpha chain, non-erythrocytic 1 isoform X4
XM_008761677.2	XP_008759899.1	spectrin alpha chain, non-erythrocytic 1 isoform X5
XM_008761678.2	XP_008759900.1	spectrin alpha chain, non-erythrocytic 1 isoform X6
NM_171983.2	NP_741984.2	spectrin alpha chain, non-erythrocytic 1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Sptan1	NP_741984.2
Danio rerio (zebrafish)	spna2	NP_001091958.1
Caenorhabditis elegans (roundworm)	spc-1	NP_001257000.1
Xenopus tropicalis (tropical clawed frog)	sptan1	NP_001090674.1
Canis lupus familiaris (dog)	SPTAN1	XP_005625341.1
Bos taurus (cattle)	SPTAN1	NP_001107628.1
Mus musculus (house mouse)	Sptan1	NP_001171138.1
Gallus gallus (chicken)	SPTAN1	NP_001036003.1
Homo sapiens (human)	SPTAN1	NP_001123910.1
	SPTAN1	XP_002800011.1
Drosophila melanogaster (fruit fly)	alpha-Spec	NP_001261286.1
Pan troglodytes (chimpanzee)	SPTAN1	XP_003312345.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006686	XP_316724.1

Gene Ontology
Bibliography

Related articles in PubMed

Critical roles of ?II spectrin in brain development and epileptic encephalopathy.
Wang Y, Ji T, Nelson AD, Glanowska K, Murphy GG, Jenkins PM, Parent JM
The Journal of clinical investigation(2018 Jan)

E-cadherin interactome complexity and robustness resolved by quantitative proteomics.
Guo Z, Neilson LJ, Zhong H, Murray PS, Zanivan S, Zaidel-Bar R
Science signaling7(354)rs7(2014 Dec)

Proteomic analysis of cerebrospinal fluid extracellular vesicles: a comprehensive dataset.
Chiasserini D, van Weering JR, Piersma SR, Pham TV, Malekzadeh A, Teunissen CE, de Wit H, Jim?nez CR
Journal of proteomics106191-204(2014 Jun)

The giant spectrin ?V couples the molecular motors to phototransduction and Usher syndrome type I proteins along their trafficking route.
Papal S, Cortese M, Legendre K, Sorusch N, Dragavon J, Sahly I, Shorte S, Wolfrum U, Petit C, El-Amraoui A
Human molecular genetics22(18)3773-88(2013 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The present study demonstrates the age-dependent intraneuronal presence of an alphaII-spectrin cleavage fragment in mammalian forebrain which is exacerbated in a transgenic model of AD.
Title: Age-related intraneuronal elevation of ?II-spectrin breakdown product SBDP120 in rodent forebrain accelerates in 3?Tg-AD mice.

Results indicate that the phosphorylation-dependent interaction between 14-3-3beta and alphaII spectrin acts as a switch between positive and negative regulation of neurite outgrowth stimulated by NCAM.
Title: Binding of alphaII spectrin to 14-3-3beta is involved in NCAM-dependent neurite outgrowth.

the embryonic mammalian heart expresses a significant amount of alpha II-spectrin with a reduced avidity for beta-spectrin and the ability to promote myocyte growth
Title: Characterization and expression of a heart-selective alternatively spliced variant of alpha II-spectrin, cardi+, during development in the rat.

Upregulation of Dpysl2 and Spna2 expression in hypertrophic cells in the penumbra regions of cortex and striatum of stroke brain indicates an early neuronal defense mechanism involving active neuronal repair, regeneration and development.
Title: Upregulation of Dpysl2 and Spna2 gene expression in the rat brain after ischemic stroke.

PDE4D4 interacts with alpha II spectrin within this membrane domain.
Title: Spectrin-anchored phosphodiesterase 4D4 restricts cAMP from disrupting microtubules and inducing endothelial cell gap formation.

The following Sptan1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sptan1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa01355	NM_171983.2 Latest version!	Rattus norvegicus spectrin, alpha, non-erythrocytic 1 (Sptan1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$2183.30 $3119.00
ORa25666	XM_008761673.2 Latest version!	Rattus norvegicus spectrin, alpha, non-erythrocytic 1 (Sptan1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
ORa25672	XM_008761678.2 Latest version!	Rattus norvegicus spectrin, alpha, non-erythrocytic 1 (Sptan1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
ORa43561	XM_008761674.2 Latest version!	Rattus norvegicus spectrin, alpha, non-erythrocytic 1 (Sptan1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
ORa43562	XM_008761675.2 Latest version!	Rattus norvegicus spectrin, alpha, non-erythrocytic 1 (Sptan1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
ORa43563	XM_008761676.2 Latest version!	Rattus norvegicus spectrin, alpha, non-erythrocytic 1 (Sptan1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
ORa43564	XM_008761677.2 Latest version!	Rattus norvegicus spectrin, alpha, non-erythrocytic 1 (Sptan1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sptan1 ( NM_171983.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_741984.2 Rattus norvegicus spectrin, alpha, non-erythrocytic 1 (Sptan1), mRNA.

Price & Availability↑

CloneID	ORa01355
Clone ID Related Accession (Same CDS sequence)	NM_171983.2
Accession Version	NM_171983.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7419bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-22
Organism	Rattus norvegicus(Norway rat)
Product	spectrin alpha chain, non-erythrocytic 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X90845.1. On or before Jan 7, 2005 this sequence version replaced XM_346647.1, NM_171983.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X90845.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGATCCAA GTGGGGTCAA AGTGCTGGAA ACAGCCGAGG ACATCCAGGA GAGACGACAG 
CAGGTTCTGG ATCGGTACCA CCGCTTCAAG GAGCTCTCTA CCTTGCGGCG GCAGAAACTG 
GAGGATTCCT ATCGCTTCCA GTTTTTTCAG AGAGATGCTG AGGAGCTGGA GAAGTGGATT 
CAGGAGAAGC TTCAAGTTGC CTCTGATGAG AACTACAAAG ACCCCACCAA CTTGCAGGGA 
AAGCTCCAGA AACACCAAGC CTTTGAAGCT GAAGTACAGG CCAACTCAGG AGCCATTGTG 
AAGCTGGATG AGACAGGAAA CTTGATGATT TCTGAAGGGC ACTTTGCATC TGAGACCATC 
CGGACACGTT TAATGGAGCT GCACCGCCAG TGGGAGTTGC TTTTGGAGAA GATGCGGGAG 
AAAGGAATCA AACTGCTGCA GGCACAGAAG CTGGTGCAGT ATTTGCGGGA GTGTGAGGAT 
GTAATGGACT GGATCAATGA CAAGGAAGCA ATTGTGACAT CTGAGGAGCT GGGCCAGGAT 
TTGGAGCATG TAGAGGTACT ACAGAAGAAG TTTGAAGAGT TTCAAACTGA TCTGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTAAACCAG TTTGCTGCCA AACTTATCCA GGAGCAGCAC 
CCGGAAGAGG AGCTGATCAA GACCAAGCAG GAGGAGGTGA ATGCAGCTTG GCAGCGACTG 
AAAGGCCTGG CTCTTCAAAG GCAGGGGAAG CTCTTTGGTG CTGCCGAGGT TCAGCGCTTT 
AACAGGGATG TAGATGAGAC CATTGGTTGG ATTAAGGAGA AAGAGCAGTT AATGGCCTCT 
GATGACTTTG GCAGAGACTT AGCAAGTGTT CAAGCTCTGC TTCGGAAGCA TGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAGGACAAG GTGAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCAACAGT CACACCCTCT GAGTGCCAAC CAGATCCAGG TGAAGCGAGA GGAACTAATT 
ACCAACTGGG AGCAGATCCG AACTCTGGCC GCAGAGAGAC ATGCACGGCT TGATGACTCA 
TACAGGCTTC AGCGCTTTCT TGCTGACTTC CGTGACCTCA CGAGCTGGGT GACTGAAATG 
AAAGCCCTCA TCAATGCAGA TGAACTGGCC AATGACGTGG CTGGTGCTGA GGCCCTGCTG 
GACAGGCATC AAGAGCACAA GGGTGAAATC GATGCTCATG AAGATAGCTT TAAGTCTGCA 
GATGAGTCTG GGCAGGCCCT ACTCGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGAGCA TCCTCTCTGA GGAGAGAGCT GCCCTGCTGG AGCTGTGGGA GCTTCGGAGG 
CAGCAGTATG AGCAGTGCAT GGACTTGCAG CTCTTCTACC GAGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAACAGGA GGCATTCCTG CTAAATGAAG ATTTGGGTGA CTCCTTAGAC 
AGTGTGGAAG CTCTTTTGAA GAAGCATGAG GACTTTGAGA AATCTCTCAG TGCCCAGGAA 
GAAAAAATCA CAGCACTTGA TGAGTTTGCA ACCAAGCTTA TTCAGAACAA CCACTACGCA 
ATGGAAGATG TAGCCACTCG ACGAGATGCT CTCCTGAGCC GCCGCAATGC CCTCCATGAG 
CGAGCCATGC ATCGCCGGGC ACAGCTGGCC GATTCCTTCC ACCTGCAGCA GTTCTTCCGC 
GATTCCGATG AGCTCAAAAG TTGGGTCAAT GAGAAGATGA AAACGGCCAC TGATGAAGCT 
TACAAAGATC CGTCCAACCT GCAAGGGAAA GTCCAAAAGC ACCAGGCTTT TGAGGCTGAG 
CTCTCAGCCA ACCAGAGCCG TATTGATGCC CTAGAGAAAG CTGGGCAAAA ACTAATAGAT 
GTGAACCACT ATGCCAAGGA AGAAGTAGCA GCTCGGATGA ATGAGGTCAT CAGTTTGTGG 
AAGAAACTTC TAGAGGCCAC AGAACTGAAA GGAGTCAAGC TCCGAGAAGC CAACCAGCAG 
CAACAATTTA ATCGCAATGT TGAGGACATT GAGTTGTGGC TGTATGAAGT TGAAGGTCAC 
TTGGCTTCAG ATGATTATGG TAAAGACCTC ACTAATGTCC AGAACCTCCA GAAGAAGCAT 
GCTCTGCTAG AGGCAGATGT TGCTGCTCAC CAGGATCGAA TTGACGGCAT CACAATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTC GATGCCGAAA ACATTAAAAA GAAGCAAGAG 
GCCCTTGTAG CTCGCTATGA GGCTCTCAAG GAACCCATGG TGGCCCGGAA GCAGAAGCTG 
GCAGATTCTC TTCGTCTGCA GCAGCTCTTC CGAGATGTGG AGGATGAGGA AACCTGGATT 
CGAGAAAAGG AGCCTATTGC TGCGTCCACT AACAGAGGCA AAGATCTTAT TGGAGTCCAG 
AATCTGCTAA AGAAGCACCA AGCTTTACAG GCAGAAATTG CTGGCCATGA ACCTCGCATC 
AAAGCAGTGA CACAAAAGGG CAATGCCATG GTGGAGGAAG GCCATTTTGC TGCTGAGGAT 
GTGAAGGCCA AACTGAGTGA GCTCAACCAG AAGTGGGAGG CACTGAAAGC CAAAGCCTCC 
CAGCGGAGGC AGGATCTGGA GGACTCACTA CAGGCCCAGC AGTACTTTGC CGACGCCAAT 
GAAGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATTGTGG GCAGTACCGA CTATGGGAAG 
GATGAAGACT CTGCTGAGGC TCTGCTCAAG AAGCATGAAG CTTTGATGTC CGATCTCAGT 
GCCTACGGCA GCAGCATTCA AGCTTTGCGA GAGCAGGCTC AGTCATGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGCAAGGAG CTGGTCTTGG CTCTCTATGA CTATCAAGAG 
AAGAGCCCTC GTGAGGTCAC CATGAAGAAA GGGGATATCC TCACCTTGCT CAACAGCACA 
AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGTCAGG GTTTTGTGCC AGCTGCGTAT 
GTGAAGAAGC TGGACCCCGC CCAGTCAGCC TCAAGGGAGA ACCTCCTGGA AGAACAGGGC 
AGCATTGCTC TGCGGCAGGG GCAGATCGAC AACCAGACAC GCATAACTAA GGAGGCCGGC 
AGTGTATCTC TGCGTATGAA ACAGGTGGAA GAACTGTATC AGTCTCTGCT GGAGCTGGGT 
GAGAAGAGAA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TCATGTTGTT CCGGGAAGCG 
AACGAGCTAC AGCAGTGGAT CAACGAGAAG GAAGCTGCTC TAACGAGTGA AGAGGTTGGC 
GCTGACTTGG AGCAGGTCGA GGTGCTGCAG AAGAAGTTCG ATGACTTCCA GAAGGATCTG 
AAAGCCAATG AGTCCCGCCT GAAGGACATT AACAAAGTGG CCGAGGACCT GGAGTCTGAA 
GGTCTCATGG CGGAAGAAGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG TATGATGCCC 
AGGGATGAAG CAGATTCCAA GACCGCCTCC CCATGGAAGT CTGCTCGACT GATGGTCCAC 
ACAGTGGCCA CCTTCAACTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTGCAACAG 
CTGGCTGAGG AACGTAGCCA GCTCCTGGGC AGTGCACACG AAGTACAGAG GTTCCACAGG 
GATGCGGATG AAACCAAAGA ATGGATTGAG GAGAAGAACC AGGCTCTGAA CACAGACAAC 
TATGGCCATG ATCTAGCTAG CGTCCAGGCC CTGCAGCGCA AACACGAAGG CTTCGAGAGG 
GACCTTGCAG CTCTTGGTGA CAAGGTGAAT TCCCTTGGGG AAACAGCCCA GAGGCTGATC 
CAGTCCCACC CTGAATCTGC AGAGGACTTA AAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGACCAGCC TAGGGAAGCG TGCAGACCAG CGCAAGGCCA AACTGGGTGA CTCCCATGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGAGGG 
TTGGTATCTT CAGATGAACT GGCCAAGGAT GTCACTGGAG CTGAGGCTTT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATTGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TATGCCAGCC CAGAGATCAA GGAGAAACTT 
GATATTCTTG ACCAGGAGCG CACAGACCTG GAGAAGGCCT GGGTTCAGCG CAGAATGATG 
CTGGACCACT GCCTGGAGTT GCAGCTGTTC CATCGAGACT GTGAGCAAGC AGAGAACTGG 
ATGGCTGCCC GGGAAGCCTT CCTAAACACA GAAGACAAAG GAGACTCGCT GGACAGTGTG 
GAGGCTCTGA TCAAAAAACA TGAAGACTTC GACAAAGCTA TCAATGTCCA GGAGGAGAAG 
ATAGCTGCCC TGCAGGCCTT TGCCGACCAG CTCATTGCTC TGGACCACTA TGCCAAGGGA 
GACATTGCAA ACCGACGCAA TGAGGTCCTG GACAGGTGGC GCCGCCTAAA AGCCCAGATG 
ATTGAGAAAA GGTCAAAGCT CGGAGAATCT CAAACACTTC AGCAGTTCAG CCGGGATGTA 
GATGAGATTG AAGCTTGGAT CAGTGAGAAG TTACAAACAG CCAGCGATGA GTCATACAAG 
GACCCCACCA ACATCCAGAG CAAGCACCAG AAGCACCAAG CCTTTGAGGC AGAACTGCAC 
GCCAATGCTG ACCGAATCCG TGGAGTTATT GACATGGGCA ACTCCCTCAT TGAGCGTGGG 
GCCTGTGCTG GCAGTGAGGA TGCTGTCAAG GCCCGCCTGG CTGCCCTTGC AGACCAGTGG 
CAGTTCCTGG TGCAGAAGTC AGCTGAGAAG AGCCAGAAGC TGAAAGAGGC CAATAAGCAG 
CAGAACTTCA ACACCGGGAT CAAAGACTTT GACTTCTGGC TTTCTGAGGT GGAGGCTCTC 
CTGGCATCTG AAGACTACGG CAAAGACCTG GCTTCCGTGA ACAACCTGCT CAAAAAGCAT 
CAGCTGCTGG AGGCAGACAT ATCGGCCCAC GAGGATCGTC TGAAGGACCT GAACAGCCAG 
GCTGACAGCC TGATGACTAG CAGTGCCTTC GACACCTCCC AAGTGAAAGA GAAGCGGGAC 
ACCATCAATG GACGCTTTCA GAAGATCAAG AGCATGGCAA CCTCCCGAAG AGCAAAACTG 
AGCGAGTCCC ATCGCCTGCA CCAGTTTTTC CGAGACATGG ATGACGAGGA GTCCTGGATC 
AAGGAGAAGA AGTTGTTAGT GAGCTCTGAG GACTATGGCA GAGACCTCAC TGGTGTTCAA 
AATCTGAGGA AGAAACACAA GCGGCTAGAA GCCGAACTGG CCGCACACGA ACCAGCCATT 
CAGGGTGTCC TGGACACGGG GAAGAAGCTG TCTGATGACA ACACCATCGG GCAGGAGGAG 
ATCCAGCAGC GTCTCGCACA GTTTGTGGAG CACTGGAAGG AACTGAAACA GCTAGCAGCT 
GCACGGGGCC AGCGGCTGGA GGAGTCCTTG GAGTATCAGC AGTTTGTGGC CAACGTGGAG 
GAGGAGGAGG CTTGGATCAA TGAGAAGATG ACCCTGGTGG CCAGCGAAGA CTACGGGGAC 
ACTCTTGCTG CCATCCAGGG CTTACTGAAG AAACATGAAG CATTTGAGAC AGACTTCACT 
GTCCACAAGG ATCGAGTGAA TGATGTCTGT ACTAATGGAC AAGACCTCAT TAAGAAGAAC 
AATCACCATG AGGAGAACAT CTCTTCAAAG ATGAAGGGTC TGAATGGTAA AGTGTCTGAC 
CTGGAGAAAG CAGCAGCTCA GCGGAAAGCG AACGTGGATG AGAACTCGGC CTTCCTTCAG 
TTCAATTGGA AGGCTGACGT GGTGGAGTCC TGGATTGGTG AAAAGGAGAA CAGCTTGAAA 
ACAGATGATT ATGGCCGAGA TCTGTCTTCT GTCCAAACTC TGCTCACCAA GCAGGAGACA 
TTTGATGCTG GCCTGCAGGC CTTCCAGCAG GAGGGCATTG CCAATATCAC TGCCCTCAAA 
GACCAGCTGC TAGCTGCCAA GCACATTCAG TCGAAGGCCA TCGAGGCCCG ACATGCCTCC 
CTCATGAAGA GGTGGACCCA GCTGTTGGCC AATTCAGCTA CCCGCAAGAA GAAGTTGCTA 
GAGGCCCAGA GTCATTTCCG AAAGGTAGAA GACCTCTTCC TGACCTTTGC CAAAAAGGCA 
TCGGCTTTCA ACAGCTGGTT TGAGAATGCA GAAGAGGACC TCACAGACCC AGTGCGCTGC 
AACTCTCTGG AAGAAATCAA AGCCCTCCGA GAGGCTCATG ATGCCTTCCG CTCATCGCTC 
AGCTCTGCGC AGGCCGACTT CAACCAGCTA GCCGAGCTGG ACCGTCAGAT CAAGAGTTTC 
CGAGTGGCCT CCAATCCCTA CACCTGGTTC ACCATGGAGG CCCTGGAAGA GACGTGGAGG 
AACCTACAGA AGATCATTAA GGAGCGAGAA CTGGAGCTGC AGAAGGAACA GCGGCGGCAG 
GAGGAGAATG ACAACGTACG CCAAGAGTTT GCCCAGCATG CCAACGCGTT CCACCAGTGG 
ATCCAGGAAA CAAGAACGTA TCTCCTCGAC GGGTCCTGCA TGGTCGAAGA GTCGGGAACT 
CTGGAATCTC AGCTTGAAGC TACCAAACGC AAGCACCAGG AGATTCGGGC CATGAGAAGT 
CAGCTGAAGA AGATTGAGGA CCTGGGTGCT GCCATGGAGG AAGCCCTCAT CCTGGACAAC 
AAGTACACTG AGCACAGCAC TGTGGGCCTG GCCCAGCAGT GGGACCAGTT AGACCAGCTG 
GGCATGCGCA TGCAGCACAA CCTGGAGCAG CAGATCCAGG CCAGGAACAC AACAGGAGTC 
ACTGAGGAGG CCCTCAAGGA GTTCAGCATG ATGTTCAAAC ACTTCGACAA GGACAAGTCT 
GGCCGGCTGA ATCATCAAGA GTTCAAATCC TGCCTTCGTT CTCTGGGTTA CGACCTGCCA 
ATGGTTGAGG AAGGAGAGCC TGATCCTGAG TTTGAGGCCA TACTGGACAC TGTTGATCCC 
AACAGGGACG GCCACGTCTC CCTGCAAGAG TACATGGCTT TCATGATCAG CCGTGAAACC 
GAGAATGTCA AGTCCAGTGA AGAGATCGAG AGTGCTTTCC GGGCCCTCAG CTCCGAGGGC 
AAGCCTTATG TGACCAAGGA GGAGCTCTAC CAGAACCTGA CCCGGGAACA AGCTGACTAC 
TGTGTCTCCC ACATGAAGCC CTATGTGGAT GGCAAGGGCC GCGAACTTCC AACTGCCTTC 
GACTACGTGG AGTTCACCCG TTCTCTCTTT GTGAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_741984.2
CDS	109..7527
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQVASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDLAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQEEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETIGWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSANQIQVKREELITNWEQIRTLAAERHARLDDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLSILSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMHRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKEEVAARMNEVISLWKKLLEATELKGVKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLSELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQGQIDNQTRITKEAGSVSLRMKQVEELYQSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGMMPRDEADSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAQRLIQSHPESAEDLKEKCTELNQAWTSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKEKLDILDQERTDLEKAWVQRRMMLDHCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIALDHYAKGDIANRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKEKRDTINGRFQKIKSMATSRRAKLSESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGQEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKANVDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHIQSKAIEARHASLMKRWTQLLANSATRKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDNVRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	NM_171983.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus spectrin, alpha, non-erythrocytic 1 (Sptan1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_171983.2

ORF Insert Sequence:

ATGGATCCAA GTGGGGTCAA AGTGCTGGAA ACAGCCGAGG ACATCCAGGA GAGACGACAG 
CAGGTTCTGG ATCGGTACCA CCGCTTCAAG GAGCTCTCTA CCTTGCGGCG GCAGAAACTG 
GAGGATTCCT ATCGCTTCCA GTTTTTTCAG AGAGATGCTG AGGAGCTGGA GAAGTGGATT 
CAGGAGAAGC TTCAAGTTGC CTCTGATGAG AACTACAAAG ACCCCACCAA CTTGCAGGGA 
AAGCTCCAGA AACACCAAGC CTTTGAAGCT GAAGTACAGG CCAACTCAGG AGCCATTGTG 
AAGCTGGATG AGACAGGAAA CTTGATGATT TCTGAAGGGC ACTTTGCATC TGAGACCATC 
CGGACACGTT TAATGGAGCT GCACCGCCAG TGGGAGTTGC TTTTGGAGAA GATGCGGGAG 
AAAGGAATCA AACTGCTGCA GGCACAGAAG CTGGTGCAGT ATTTGCGGGA GTGTGAGGAT 
GTAATGGACT GGATCAATGA CAAGGAAGCA ATTGTGACAT CTGAGGAGCT GGGCCAGGAT 
TTGGAGCATG TAGAGGTACT ACAGAAGAAG TTTGAAGAGT TTCAAACTGA TCTGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTAAACCAG TTTGCTGCCA AACTTATCCA GGAGCAGCAC 
CCGGAAGAGG AGCTGATCAA GACCAAGCAG GAGGAGGTGA ATGCAGCTTG GCAGCGACTG 
AAAGGCCTGG CTCTTCAAAG GCAGGGGAAG CTCTTTGGTG CTGCCGAGGT TCAGCGCTTT 
AACAGGGATG TAGATGAGAC CATTGGTTGG ATTAAGGAGA AAGAGCAGTT AATGGCCTCT 
GATGACTTTG GCAGAGACTT AGCAAGTGTT CAAGCTCTGC TTCGGAAGCA TGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAGGACAAG GTGAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCAACAGT CACACCCTCT GAGTGCCAAC CAGATCCAGG TGAAGCGAGA GGAACTAATT 
ACCAACTGGG AGCAGATCCG AACTCTGGCC GCAGAGAGAC ATGCACGGCT TGATGACTCA 
TACAGGCTTC AGCGCTTTCT TGCTGACTTC CGTGACCTCA CGAGCTGGGT GACTGAAATG 
AAAGCCCTCA TCAATGCAGA TGAACTGGCC AATGACGTGG CTGGTGCTGA GGCCCTGCTG 
GACAGGCATC AAGAGCACAA GGGTGAAATC GATGCTCATG AAGATAGCTT TAAGTCTGCA 
GATGAGTCTG GGCAGGCCCT ACTCGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGAGCA TCCTCTCTGA GGAGAGAGCT GCCCTGCTGG AGCTGTGGGA GCTTCGGAGG 
CAGCAGTATG AGCAGTGCAT GGACTTGCAG CTCTTCTACC GAGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAACAGGA GGCATTCCTG CTAAATGAAG ATTTGGGTGA CTCCTTAGAC 
AGTGTGGAAG CTCTTTTGAA GAAGCATGAG GACTTTGAGA AATCTCTCAG TGCCCAGGAA 
GAAAAAATCA CAGCACTTGA TGAGTTTGCA ACCAAGCTTA TTCAGAACAA CCACTACGCA 
ATGGAAGATG TAGCCACTCG ACGAGATGCT CTCCTGAGCC GCCGCAATGC CCTCCATGAG 
CGAGCCATGC ATCGCCGGGC ACAGCTGGCC GATTCCTTCC ACCTGCAGCA GTTCTTCCGC 
GATTCCGATG AGCTCAAAAG TTGGGTCAAT GAGAAGATGA AAACGGCCAC TGATGAAGCT 
TACAAAGATC CGTCCAACCT GCAAGGGAAA GTCCAAAAGC ACCAGGCTTT TGAGGCTGAG 
CTCTCAGCCA ACCAGAGCCG TATTGATGCC CTAGAGAAAG CTGGGCAAAA ACTAATAGAT 
GTGAACCACT ATGCCAAGGA AGAAGTAGCA GCTCGGATGA ATGAGGTCAT CAGTTTGTGG 
AAGAAACTTC TAGAGGCCAC AGAACTGAAA GGAGTCAAGC TCCGAGAAGC CAACCAGCAG 
CAACAATTTA ATCGCAATGT TGAGGACATT GAGTTGTGGC TGTATGAAGT TGAAGGTCAC 
TTGGCTTCAG ATGATTATGG TAAAGACCTC ACTAATGTCC AGAACCTCCA GAAGAAGCAT 
GCTCTGCTAG AGGCAGATGT TGCTGCTCAC CAGGATCGAA TTGACGGCAT CACAATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTC GATGCCGAAA ACATTAAAAA GAAGCAAGAG 
GCCCTTGTAG CTCGCTATGA GGCTCTCAAG GAACCCATGG TGGCCCGGAA GCAGAAGCTG 
GCAGATTCTC TTCGTCTGCA GCAGCTCTTC CGAGATGTGG AGGATGAGGA AACCTGGATT 
CGAGAAAAGG AGCCTATTGC TGCGTCCACT AACAGAGGCA AAGATCTTAT TGGAGTCCAG 
AATCTGCTAA AGAAGCACCA AGCTTTACAG GCAGAAATTG CTGGCCATGA ACCTCGCATC 
AAAGCAGTGA CACAAAAGGG CAATGCCATG GTGGAGGAAG GCCATTTTGC TGCTGAGGAT 
GTGAAGGCCA AACTGAGTGA GCTCAACCAG AAGTGGGAGG CACTGAAAGC CAAAGCCTCC 
CAGCGGAGGC AGGATCTGGA GGACTCACTA CAGGCCCAGC AGTACTTTGC CGACGCCAAT 
GAAGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATTGTGG GCAGTACCGA CTATGGGAAG 
GATGAAGACT CTGCTGAGGC TCTGCTCAAG AAGCATGAAG CTTTGATGTC CGATCTCAGT 
GCCTACGGCA GCAGCATTCA AGCTTTGCGA GAGCAGGCTC AGTCATGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGCAAGGAG CTGGTCTTGG CTCTCTATGA CTATCAAGAG 
AAGAGCCCTC GTGAGGTCAC CATGAAGAAA GGGGATATCC TCACCTTGCT CAACAGCACA 
AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGTCAGG GTTTTGTGCC AGCTGCGTAT 
GTGAAGAAGC TGGACCCCGC CCAGTCAGCC TCAAGGGAGA ACCTCCTGGA AGAACAGGGC 
AGCATTGCTC TGCGGCAGGG GCAGATCGAC AACCAGACAC GCATAACTAA GGAGGCCGGC 
AGTGTATCTC TGCGTATGAA ACAGGTGGAA GAACTGTATC AGTCTCTGCT GGAGCTGGGT 
GAGAAGAGAA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TCATGTTGTT CCGGGAAGCG 
AACGAGCTAC AGCAGTGGAT CAACGAGAAG GAAGCTGCTC TAACGAGTGA AGAGGTTGGC 
GCTGACTTGG AGCAGGTCGA GGTGCTGCAG AAGAAGTTCG ATGACTTCCA GAAGGATCTG 
AAAGCCAATG AGTCCCGCCT GAAGGACATT AACAAAGTGG CCGAGGACCT GGAGTCTGAA 
GGTCTCATGG CGGAAGAAGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG TATGATGCCC 
AGGGATGAAG CAGATTCCAA GACCGCCTCC CCATGGAAGT CTGCTCGACT GATGGTCCAC 
ACAGTGGCCA CCTTCAACTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTGCAACAG 
CTGGCTGAGG AACGTAGCCA GCTCCTGGGC AGTGCACACG AAGTACAGAG GTTCCACAGG 
GATGCGGATG AAACCAAAGA ATGGATTGAG GAGAAGAACC AGGCTCTGAA CACAGACAAC 
TATGGCCATG ATCTAGCTAG CGTCCAGGCC CTGCAGCGCA AACACGAAGG CTTCGAGAGG 
GACCTTGCAG CTCTTGGTGA CAAGGTGAAT TCCCTTGGGG AAACAGCCCA GAGGCTGATC 
CAGTCCCACC CTGAATCTGC AGAGGACTTA AAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGACCAGCC TAGGGAAGCG TGCAGACCAG CGCAAGGCCA AACTGGGTGA CTCCCATGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGAGGG 
TTGGTATCTT CAGATGAACT GGCCAAGGAT GTCACTGGAG CTGAGGCTTT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATTGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TATGCCAGCC CAGAGATCAA GGAGAAACTT 
GATATTCTTG ACCAGGAGCG CACAGACCTG GAGAAGGCCT GGGTTCAGCG CAGAATGATG 
CTGGACCACT GCCTGGAGTT GCAGCTGTTC CATCGAGACT GTGAGCAAGC AGAGAACTGG 
ATGGCTGCCC GGGAAGCCTT CCTAAACACA GAAGACAAAG GAGACTCGCT GGACAGTGTG 
GAGGCTCTGA TCAAAAAACA TGAAGACTTC GACAAAGCTA TCAATGTCCA GGAGGAGAAG 
ATAGCTGCCC TGCAGGCCTT TGCCGACCAG CTCATTGCTC TGGACCACTA TGCCAAGGGA 
GACATTGCAA ACCGACGCAA TGAGGTCCTG GACAGGTGGC GCCGCCTAAA AGCCCAGATG 
ATTGAGAAAA GGTCAAAGCT CGGAGAATCT CAAACACTTC AGCAGTTCAG CCGGGATGTA 
GATGAGATTG AAGCTTGGAT CAGTGAGAAG TTACAAACAG CCAGCGATGA GTCATACAAG 
GACCCCACCA ACATCCAGAG CAAGCACCAG AAGCACCAAG CCTTTGAGGC AGAACTGCAC 
GCCAATGCTG ACCGAATCCG TGGAGTTATT GACATGGGCA ACTCCCTCAT TGAGCGTGGG 
GCCTGTGCTG GCAGTGAGGA TGCTGTCAAG GCCCGCCTGG CTGCCCTTGC AGACCAGTGG 
CAGTTCCTGG TGCAGAAGTC AGCTGAGAAG AGCCAGAAGC TGAAAGAGGC CAATAAGCAG 
CAGAACTTCA ACACCGGGAT CAAAGACTTT GACTTCTGGC TTTCTGAGGT GGAGGCTCTC 
CTGGCATCTG AAGACTACGG CAAAGACCTG GCTTCCGTGA ACAACCTGCT CAAAAAGCAT 
CAGCTGCTGG AGGCAGACAT ATCGGCCCAC GAGGATCGTC TGAAGGACCT GAACAGCCAG 
GCTGACAGCC TGATGACTAG CAGTGCCTTC GACACCTCCC AAGTGAAAGA GAAGCGGGAC 
ACCATCAATG GACGCTTTCA GAAGATCAAG AGCATGGCAA CCTCCCGAAG AGCAAAACTG 
AGCGAGTCCC ATCGCCTGCA CCAGTTTTTC CGAGACATGG ATGACGAGGA GTCCTGGATC 
AAGGAGAAGA AGTTGTTAGT GAGCTCTGAG GACTATGGCA GAGACCTCAC TGGTGTTCAA 
AATCTGAGGA AGAAACACAA GCGGCTAGAA GCCGAACTGG CCGCACACGA ACCAGCCATT 
CAGGGTGTCC TGGACACGGG GAAGAAGCTG TCTGATGACA ACACCATCGG GCAGGAGGAG 
ATCCAGCAGC GTCTCGCACA GTTTGTGGAG CACTGGAAGG AACTGAAACA GCTAGCAGCT 
GCACGGGGCC AGCGGCTGGA GGAGTCCTTG GAGTATCAGC AGTTTGTGGC CAACGTGGAG 
GAGGAGGAGG CTTGGATCAA TGAGAAGATG ACCCTGGTGG CCAGCGAAGA CTACGGGGAC 
ACTCTTGCTG CCATCCAGGG CTTACTGAAG AAACATGAAG CATTTGAGAC AGACTTCACT 
GTCCACAAGG ATCGAGTGAA TGATGTCTGT ACTAATGGAC AAGACCTCAT TAAGAAGAAC 
AATCACCATG AGGAGAACAT CTCTTCAAAG ATGAAGGGTC TGAATGGTAA AGTGTCTGAC 
CTGGAGAAAG CAGCAGCTCA GCGGAAAGCG AACGTGGATG AGAACTCGGC CTTCCTTCAG 
TTCAATTGGA AGGCTGACGT GGTGGAGTCC TGGATTGGTG AAAAGGAGAA CAGCTTGAAA 
ACAGATGATT ATGGCCGAGA TCTGTCTTCT GTCCAAACTC TGCTCACCAA GCAGGAGACA 
TTTGATGCTG GCCTGCAGGC CTTCCAGCAG GAGGGCATTG CCAATATCAC TGCCCTCAAA 
GACCAGCTGC TAGCTGCCAA GCACATTCAG TCGAAGGCCA TCGAGGCCCG ACATGCCTCC 
CTCATGAAGA GGTGGACCCA GCTGTTGGCC AATTCAGCTA CCCGCAAGAA GAAGTTGCTA 
GAGGCCCAGA GTCATTTCCG AAAGGTAGAA GACCTCTTCC TGACCTTTGC CAAAAAGGCA 
TCGGCTTTCA ACAGCTGGTT TGAGAATGCA GAAGAGGACC TCACAGACCC AGTGCGCTGC 
AACTCTCTGG AAGAAATCAA AGCCCTCCGA GAGGCTCATG ATGCCTTCCG CTCATCGCTC 
AGCTCTGCGC AGGCCGACTT CAACCAGCTA GCCGAGCTGG ACCGTCAGAT CAAGAGTTTC 
CGAGTGGCCT CCAATCCCTA CACCTGGTTC ACCATGGAGG CCCTGGAAGA GACGTGGAGG 
AACCTACAGA AGATCATTAA GGAGCGAGAA CTGGAGCTGC AGAAGGAACA GCGGCGGCAG 
GAGGAGAATG ACAACGTACG CCAAGAGTTT GCCCAGCATG CCAACGCGTT CCACCAGTGG 
ATCCAGGAAA CAAGAACGTA TCTCCTCGAC GGGTCCTGCA TGGTCGAAGA GTCGGGAACT 
CTGGAATCTC AGCTTGAAGC TACCAAACGC AAGCACCAGG AGATTCGGGC CATGAGAAGT 
CAGCTGAAGA AGATTGAGGA CCTGGGTGCT GCCATGGAGG AAGCCCTCAT CCTGGACAAC 
AAGTACACTG AGCACAGCAC TGTGGGCCTG GCCCAGCAGT GGGACCAGTT AGACCAGCTG 
GGCATGCGCA TGCAGCACAA CCTGGAGCAG CAGATCCAGG CCAGGAACAC AACAGGAGTC 
ACTGAGGAGG CCCTCAAGGA GTTCAGCATG ATGTTCAAAC ACTTCGACAA GGACAAGTCT 
GGCCGGCTGA ATCATCAAGA GTTCAAATCC TGCCTTCGTT CTCTGGGTTA CGACCTGCCA 
ATGGTTGAGG AAGGAGAGCC TGATCCTGAG TTTGAGGCCA TACTGGACAC TGTTGATCCC 
AACAGGGACG GCCACGTCTC CCTGCAAGAG TACATGGCTT TCATGATCAG CCGTGAAACC 
GAGAATGTCA AGTCCAGTGA AGAGATCGAG AGTGCTTTCC GGGCCCTCAG CTCCGAGGGC 
AAGCCTTATG TGACCAAGGA GGAGCTCTAC CAGAACCTGA CCCGGGAACA AGCTGACTAC 
TGTGTCTCCC ACATGAAGCC CTATGTGGAT GGCAAGGGCC GCGAACTTCC AACTGCCTTC 
GACTACGTGG AGTTCACCCG TTCTCTCTTT GTGAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sptan1 ( XM_008761673.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008759895.1 Rattus norvegicus spectrin, alpha, non-erythrocytic 1 (Sptan1), transcript variant X1, mRNA.

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CloneID	ORa25666
Clone ID Related Accession (Same CDS sequence)	XM_008761673.2
Accession Version	XM_008761673.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7497bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008761673.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCAA GTGGGGTCAA AGTGCTGGAA ACAGCCGAGG ACATCCAGGA GAGACGACAG 
CAGGTTCTGG ATCGGTACCA CCGCTTCAAG GAGCTCTCTA CCTTGCGGCG GCAGAAACTG 
GAGGATTCCT ATCGCTTCCA GTTTTTTCAG AGAGATGCTG AGGAGCTGGA GAAGTGGATT 
CAGGAGAAGC TTCAAGTTGC CTCTGATGAG AACTACAAAG ACCCCACCAA CTTGCAGGGA 
AAGCTCCAGA AACACCAAGC CTTTGAAGCT GAAGTACAGG CCAACTCAGG AGCCATTGTG 
AAGCTGGATG AGACAGGAAA CTTGATGATT TCTGAAGGGC ACTTTGCATC TGAGACCATC 
CGGACACGTT TAATGGAGCT GCACCGCCAG TGGGAGTTGC TTTTGGAGAA GATGCGGGAG 
AAAGGAATCA AACTGCTGCA GGCACAGAAG CTGGTGCAGT ATTTGCGGGA GTGTGAGGAT 
GTAATGGACT GGATCAATGA CAAGGAAGCA ATTGTGACGT CTGAGGAGCT GGGCCAGGAT 
TTGGAGCATG TAGAGGTACT ACAGAAGAAG TTTGAAGAGT TTCAAACTGA TCTGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTAAACCAG TTTGCTGCCA AACTTATCCA GGAGCAGCAC 
CCGGAAGAGG AGCTGATCAA GACCAAGCAG GAGGAGGTGA ATGCAGCTTG GCAGCGACTG 
AAAGGCCTGG CTCTTCAAAG GCAGGGGAAG CTCTTTGGTG CTGCCGAGGT TCAGCGCTTT 
AACAGGGATG TAGATGAGAC CATTGGTTGG ATTAAGGAGA AAGAGCAGTT AATGGCCTCT 
GATGACTTTG GCAGAGACTT AGCAAGTGTT CAAGCTCTGC TTCGGAAGCA TGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAGGACAAG GTGAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCAACAGT CACACCCTCT GAGTGCCAAC CAGATCCAGG TGAAGCGAGA GGAACTAATT 
ACCAACTGGG AGCAGATCCG AACTCTGGCC GCAGAGAGAC ATGCACGGCT TGATGACTCA 
TACAGGCTTC AGCGCTTTCT TGCTGACTTC CGTGACCTCA CGAGCTGGGT GACTGAAATG 
AAAGCCCTCA TCAATGCAGA TGAACTGGCC AATGACGTGG CTGGTGCTGA GGCCCTGCTG 
GACAGGCATC AAGAGCACAA GGGTGAAATC GATGCTCATG AAGATAGCTT TAAGTCTGCA 
GATGAGTCTG GGCAGGCCCT ACTCGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGAGCA TCCTCTCTGA GGAGAGAGCT GCCCTGCTGG AGCTGTGGGA GCTTCGGAGG 
CAACAGTATG AGCAGTGCAT GGACTTGCAG CTCTTCTACC GAGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAACAGGA GGCATTCCTG CTAAATGAAG ATTTGGGTGA CTCCTTAGAC 
AGTGTGGAAG CTCTTTTGAA GAAGCATGAG GACTTTGAGA AATCTCTCAG TGCCCAGGAA 
GAAAAAATCA CAGCACTTGA TGAGTTTGCA ACCAAGCTTA TTCAGAACAA CCACTACGCA 
ATGGAAGATG TAGCCACTCG ACGAGATGCT CTCCTGAGCC GCCGCAATGC CCTCCATGAG 
CGAGCCATGC ATCGCCGGGC ACAGCTGGCC GATTCCTTCC ACCTGCAGCA GTTCTTCCGC 
GATTCCGATG AGCTCAAAAG TTGGGTCAAT GAGAAGATGA AAACGGCCAC TGATGAAGCT 
TACAAAGATC CGTCCAACCT GCAAGGGAAA GTCCAAAAGC ACCAGGCTTT TGAGGCTGAG 
CTCTCAGCCA ACCAGAGCCG TATTGATGCC CTAGAGAAAG CTGGGCAAAA ACTAATAGAT 
GTGAACCACT ATGCCAAGGA AGAAGTAGCA GCTCGGATGA ATGAGGTCAT CAGTTTGTGG 
AAGAAACTTC TAGAGGCCAC AGAACTGAAA GGAGTCAAGC TCCGAGAAGC CAACCAGCAG 
CAACAATTTA ATCGAAATGT TGAGGACATT GAGTTGTGGC TGTATGAAGT TGAAGGTCAC 
TTGGCTTCAG ATGATTATGG TAAAGACCTC ACTAATGTCC AGAACCTCCA GAAGAAGCAT 
GCTCTGCTAG AGGCAGATGT TGCTGCTCAC CAGGATCGAA TTGACGGCAT CACAATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTC GATGCCGAAA ACATTAAAAA GAAGCAAGAG 
GCCCTTGTAG CTCGCTATGA GGCTCTCAAG GAACCCATGG TGGCCCGGAA GCAGAAGCTG 
GCAGATTCTC TTCGTCTGCA GCAGCTCTTC CGAGATGTGG AGGATGAGGA AACCTGGATT 
CGAGAAAAGG AGCCTATTGC TGCGTCCACT AACAGAGGCA AAGATCTTAT TGGAGTCCAG 
AATCTGCTAA AGAAGCACCA AGCTTTACAG GCAGAAATTG CTGGCCATGA ACCTCGCATC 
AAAGCAGTGA CACAAAAGGG CAATGCCATG GTGGAGGAAG GCCATTTTGC TGCTGAGGAT 
GTGAAGGCCA AACTGAGTGA GCTCAACCAG AAGTGGGAGG CACTGAAAGC CAAAGCCTCC 
CAGCGGAGGC AGGATCTGGA GGACTCACTA CAGGCCCAGC AGTACTTTGC CGACGCCAAT 
GAAGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATTGTGG GCAGTACCGA CTATGGGAAG 
GATGAAGACT CTGCTGAGGC TCTGCTCAAG AAGCATGAAG CTTTGATGTC CGATCTCAGT 
GCCTACGGCA GCAGCATTCA AGCTTTGCGA GAGCAGGCTC AGTCATGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGCAAGGAG CTGGTCTTGG CTCTCTATGA CTATCAAGAG 
AAGAGCCCTC GTGAGGTCAC CATGAAGAAA GGGGATATCC TCACCTTGCT CAACAGCACA 
AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGTCAGG GTTTTGTGCC AGCTGCGTAT 
GTGAAGAAGC TGGACCCCGC CCAGTCAGCC TCAAGGGAGA ACCTCCTGGA AGAACAGGGC 
AGCATTGCTC TGCGGCAGGG GCAGATCGAC AACCAGACAC GCATAACTAA GGAGGCCGGC 
AGTGTATCTC TGCGTATGAA ACAGGTGGAA GAACTGTATC AGTCTCTGCT GGAGCTGGGT 
GAGAAGAGAA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TCATGTTGTT CCGGGAAGCG 
AACGAGCTAC AGCAGTGGAT CAACGAGAAG GAAGCTGCTC TAACGAGTGA AGAGGTTGGC 
GCTGACTTGG AGCAGGTCGA GGTGCTGCAG AAGAAGTTCG ATGACTTCCA GAAGGATCTG 
AAAGCCAATG AGTCCCGGCT GAAGGACATT AACAAAGTGG CCGAGGACCT GGAGTCTGAA 
GGTCTCATGG CGGAAGAAGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG TATGATGCCC 
AGGGATGAAG CAGATTCCAA GACCGCCTCC CCATGGAAGT CTGCTCGACT GATGGTCCAC 
ACAGTGGCCA CCTTCAACTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTGCAACAG 
CTGGCTGAGG AACGTAGCCA GCTCCTGGGC AGTGCACACG AAGTACAGAG GTTCCACAGG 
GATGCGGATG AAACCAAAGA ATGGATTGAG GAGAAGAACC AGGCTCTGAA CACAGACAAC 
TATGGCCATG ATCTAGCTAG CGTCCAGGCC CTGCAGCGCA AACACGAAGG CTTCGAGAGG 
GACCTTGCAG CTCTTGGTGA CAAGGTGAAT TCCCTTGGGG AAACAGCCCA GAGGCTGATC 
CAGTCCCACC CTGAATCTGC AGAGGACTTA AAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGACCAGCC TAGGGAAGCG TGCAGACCAG CGCAAGGCCA AACTGGGTGA CTCCCATGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGAGGG 
TTGGTATCTT CAGATGAACT GGCCAAGGAT GTCACTGGAG CTGAGGCTTT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATTGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TATGCCAGCC CAGAGATCAA GGAGAAACTT 
GATATTCTTG ACCAGGAGCG CACAGACCTG GAGAAGGCCT GGGTTCAGCG CAGAATGATG 
CTGGACCACT GCCTGGAGTT GCAGCTGTTC CATCGAGACT GTGAGCAAGC AGAGAACTGG 
ATGGCTGCCC GGGAAGCCTT CCTAAACACA GAAGACAAAG GAGACTCGCT GGACAGTGTG 
GAGGCTCTGA TCAAAAAACA TGAAGACTTC GACAAAGCTA TCAATGTCCA GGAGGAGAAG 
ATAGCTGCCC TGCAGGCCTT TGCCGACCAG CTCATTGCTG TGGACCACTA TGCCAAGGGA 
GACATTGCAA ACCGACGCAA TGAGGTCCTG GACAGGTGGC GCCGCCTAAA AGCCCAGATG 
ATTGAGAAAA GGTCAAAGCT CGGAGAATCT CAAACACTTC AGCAGTTCAG CCGGGATGTA 
GATGAGATTG AAGCCTGGAT CAGTGAGAAG TTACAAACAG CCAGCGATGA GTCATACAAG 
GACCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ACCAGAAGCA CCAAGCCTTT 
GAGGCAGAAC TGCACGCCAA TGCTGACCGA ATCCGTGGAG TTATTGACAT GGGCAACTCC 
CTCATTGAGC GTGGGGCCTG TGCTGGCAGT GAGGATGCTG TCAAGGCCCG CCTGGCTGCC 
CTTGCAGACC AGTGGCAGTT CCTGGTGCAG AAGTCAGCTG AGAAGAGCCA GAAGCTGAAA 
GAGGCCAATA AGCAGCAGAA CTTCAACACC GGGATCAAAG ACTTTGACTT CTGGCTTTCT 
GAGGTGGAGG CTCTCCTGGC ATCTGAAGAC TACGGCAAAG ACCTGGCTTC CGTGAACAAC 
CTGCTCAAAA AGCATCAGCT GCTGGAGGCA GACATATCGG CCCACGAGGA TCGTCTGAAG 
GACCTGAACA GCCAGGCTGA CAGCCTGATG ACTAGCAGTG CCTTCGACAC CTCCCAAGTG 
AAAGAGAAGC GGGACACCAT CAATGGACGC TTTCAGAAGA TCAAGAGCAT GGCAACCTCC 
CGAAGAGCAA AACTGAGCGA GTCCCATCGC CTGCACCAGT TTTTCCGAGA CATGGATGAC 
GAGGAGTCCT GGATCAAGGA GAAGAAGTTG TTAGTGAGCT CTGAGGACTA TGGCAGAGAC 
CTCACTGGTG TTCAAAATCT GAGGAAGAAA CACAAGCGGC TAGAAGCCGA ACTGGCCGCA 
CACGAACCAG CCATTCAGGG TGTCCTGGAC ACGGGGAAGA AGCTGTCTGA TGACAACACC 
ATCGGGCAGG AGGAGATCCA GCAGCGTCTC GCACAGTTTG TGGAGCACTG GAAGGAACTG 
AAACAGCTAG CAGCTGCACG GGGCCAGCGG CTGGAGGAGT CCTTGGAGTA TCAGCAGTTT 
GTGGCCAACG TGGAGGAGGA GGAGGCTTGG ATCAATGAGA AGATGACCCT GGTGGCCAGC 
GAAGACTACG GGGACACTCT TGCTGCCATC CAGGGCTTAC TGAAGAAACA TGAAGCTTTT 
GAGACAGACT TCACTGTCCA CAAGGATCGA GTGAATGATG TCTGTACTAA TGGACAAGAC 
CTCATTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CAAAGATGAA GGGTCTGAAT 
GGTAAAGTGT CTGACCTGGA GAAAGCAGCA GCTCAGAGGA AAGCGAAGCT GGATGAGAAC 
TCGGCCTTCC TTCAGTTCAA TTGGAAGGCT GACGTGGTGG AGTCCTGGAT TGGTGAAAAG 
GAGAACAGCT TGAAAACAGA TGATTATGGC CGAGATCTGT CTTCTGTCCA AACTCTGCTC 
ACCAAGCAGG AGACATTTGA TGCTGGCCTG CAGGCCTTCC AGCAGGAGGG CATTGCCAAT 
ATCACTGCCC TCAAAGACCA GCTGCTAGCT GCCAAGCACA TTCAGTCGAA GGCCATCGAG 
GCCCGACATG CCTCCCTCAT GAAGAGGTGG ACCCAGCTGT TGGCCAATTC AGCTACCCGC 
AAGAAGAAGT TGCTAGAGGC CCAGAGTCAT TTCCGAAAGG TAGAAGACCT CTTCCTGACC 
TTTGCCAAAA AGGCATCGGC TTTCAACAGC TGGTTTGAGA ATGCAGAAGA GGACCTCACA 
GACCCAGTGC GCTGCAACTC TCTGGAAGAA ATCAAAGCCC TCCGAGAGGC TCATGATGCC 
TTCCGCTCAT CGCTCAGCTC TGCGCAGGCC GACTTCAACC AGCTAGCCGA GCTGGACCGT 
CAGATCAAGA GTTTCCGAGT GGCCTCCAAT CCCTACACCT GGTTCACCAT GGAGGCCCTG 
GAAGAGACGT GGAGGAACCT ACAGAAGATC ATTAAGGAGC GAGAACTGGA GCTGCAGAAG 
GAACAGCGGC GGCAGGAGGA GAATGACAAG CTACGCCAAG AGTTTGCCCA GCATGCCAAC 
GCGTTCCACC AGTGGATCCA GGAAACAAGA ACGTATCTCC TCGACGGCAT AGCATATCGT 
CGGGTCATTC GTGTCTATCA GTATGAAGTT GGGGATGATC TGTCTGGAAG GTCCTGCATG 
GTCGAAGAGT CGGGAACTCT GGAATCTCAG CTTGAAGCTA CCAAACGCAA GCACCAGGAG 
ATTCGGGCCA TGAGAAGTCA GCTGAAGAAG ATTGAGGACC TGGGGGCTGC CATGGAGGAA 
GCCCTCATCC TGGACAACAA GTACACTGAG CACAGCACTG TGGGCCTGGC CCAGCAGTGG 
GACCAGTTAG ACCAGCTGGG CATGCGCATG CAGCACAACC TGGAGCAGCA GATCCAGGCC 
AGGAACACAA CAGGAGTCAC TGAGGAGGCC CTCAAGGAGT TCAGCATGAT GTTCAAACAC 
TTCGACAAGG ACAAGTCTGG CCGGCTGAAT CATCAAGAGT TCAAATCCTG CCTTCGTTCT 
CTGGGTTACG ACCTGCCAAT GGTTGAGGAA GGAGAGCCTG ATCCTGAGTT TGAGGCCATA 
CTGGACACTG TTGATCCCAA CAGGGACGGC CACGTCTCCC TGCAAGAGTA CATGGCTTTC 
ATGATCAGCC GTGAAACCGA GAATGTCAAG TCCAGTGAAG AGATCGAGAG TGCTTTCCGG 
GCCCTCAGCT CCGAGGGCAA GCCTTATGTG ACCAAGGAGG AGCTCTACCA GAACCTGACC 
CGGGAACAAG CTGACTACTG TGTCTCCCAC ATGAAGCCCT ATGTGGATGG CAAGGGCCGC 
GAACTTCCAA CTGCCTTCGA CTACGTGGAG TTCACCCGCT CTCTCTTTGT GAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008759895.1
CDS	112..7608
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQVASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDLAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQEEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETIGWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSANQIQVKREELITNWEQIRTLAAERHARLDDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLSILSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMHRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKEEVAARMNEVISLWKKLLEATELKGVKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLSELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQGQIDNQTRITKEAGSVSLRMKQVEELYQSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGMMPRDEADSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAQRLIQSHPESAEDLKEKCTELNQAWTSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKEKLDILDQERTDLEKAWVQRRMMLDHCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAVDHYAKGDIANRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKEKRDTINGRFQKIKSMATSRRAKLSESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGQEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHIQSKAIEARHASLMKRWTQLLANSATRKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGIAYRRVIRVYQYEVGDDLSGRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_008761673.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus spectrin, alpha, non-erythrocytic 1 (Sptan1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008761673.2

ORF Insert Sequence:

ATGGATCCAA GTGGGGTCAA AGTGCTGGAA ACAGCCGAGG ACATCCAGGA GAGACGACAG 
CAGGTTCTGG ATCGGTACCA CCGCTTCAAG GAGCTCTCTA CCTTGCGGCG GCAGAAACTG 
GAGGATTCCT ATCGCTTCCA GTTTTTTCAG AGAGATGCTG AGGAGCTGGA GAAGTGGATT 
CAGGAGAAGC TTCAAGTTGC CTCTGATGAG AACTACAAAG ACCCCACCAA CTTGCAGGGA 
AAGCTCCAGA AACACCAAGC CTTTGAAGCT GAAGTACAGG CCAACTCAGG AGCCATTGTG 
AAGCTGGATG AGACAGGAAA CTTGATGATT TCTGAAGGGC ACTTTGCATC TGAGACCATC 
CGGACACGTT TAATGGAGCT GCACCGCCAG TGGGAGTTGC TTTTGGAGAA GATGCGGGAG 
AAAGGAATCA AACTGCTGCA GGCACAGAAG CTGGTGCAGT ATTTGCGGGA GTGTGAGGAT 
GTAATGGACT GGATCAATGA CAAGGAAGCA ATTGTGACGT CTGAGGAGCT GGGCCAGGAT 
TTGGAGCATG TAGAGGTACT ACAGAAGAAG TTTGAAGAGT TTCAAACTGA TCTGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTAAACCAG TTTGCTGCCA AACTTATCCA GGAGCAGCAC 
CCGGAAGAGG AGCTGATCAA GACCAAGCAG GAGGAGGTGA ATGCAGCTTG GCAGCGACTG 
AAAGGCCTGG CTCTTCAAAG GCAGGGGAAG CTCTTTGGTG CTGCCGAGGT TCAGCGCTTT 
AACAGGGATG TAGATGAGAC CATTGGTTGG ATTAAGGAGA AAGAGCAGTT AATGGCCTCT 
GATGACTTTG GCAGAGACTT AGCAAGTGTT CAAGCTCTGC TTCGGAAGCA TGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAGGACAAG GTGAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCAACAGT CACACCCTCT GAGTGCCAAC CAGATCCAGG TGAAGCGAGA GGAACTAATT 
ACCAACTGGG AGCAGATCCG AACTCTGGCC GCAGAGAGAC ATGCACGGCT TGATGACTCA 
TACAGGCTTC AGCGCTTTCT TGCTGACTTC CGTGACCTCA CGAGCTGGGT GACTGAAATG 
AAAGCCCTCA TCAATGCAGA TGAACTGGCC AATGACGTGG CTGGTGCTGA GGCCCTGCTG 
GACAGGCATC AAGAGCACAA GGGTGAAATC GATGCTCATG AAGATAGCTT TAAGTCTGCA 
GATGAGTCTG GGCAGGCCCT ACTCGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGAGCA TCCTCTCTGA GGAGAGAGCT GCCCTGCTGG AGCTGTGGGA GCTTCGGAGG 
CAACAGTATG AGCAGTGCAT GGACTTGCAG CTCTTCTACC GAGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAACAGGA GGCATTCCTG CTAAATGAAG ATTTGGGTGA CTCCTTAGAC 
AGTGTGGAAG CTCTTTTGAA GAAGCATGAG GACTTTGAGA AATCTCTCAG TGCCCAGGAA 
GAAAAAATCA CAGCACTTGA TGAGTTTGCA ACCAAGCTTA TTCAGAACAA CCACTACGCA 
ATGGAAGATG TAGCCACTCG ACGAGATGCT CTCCTGAGCC GCCGCAATGC CCTCCATGAG 
CGAGCCATGC ATCGCCGGGC ACAGCTGGCC GATTCCTTCC ACCTGCAGCA GTTCTTCCGC 
GATTCCGATG AGCTCAAAAG TTGGGTCAAT GAGAAGATGA AAACGGCCAC TGATGAAGCT 
TACAAAGATC CGTCCAACCT GCAAGGGAAA GTCCAAAAGC ACCAGGCTTT TGAGGCTGAG 
CTCTCAGCCA ACCAGAGCCG TATTGATGCC CTAGAGAAAG CTGGGCAAAA ACTAATAGAT 
GTGAACCACT ATGCCAAGGA AGAAGTAGCA GCTCGGATGA ATGAGGTCAT CAGTTTGTGG 
AAGAAACTTC TAGAGGCCAC AGAACTGAAA GGAGTCAAGC TCCGAGAAGC CAACCAGCAG 
CAACAATTTA ATCGAAATGT TGAGGACATT GAGTTGTGGC TGTATGAAGT TGAAGGTCAC 
TTGGCTTCAG ATGATTATGG TAAAGACCTC ACTAATGTCC AGAACCTCCA GAAGAAGCAT 
GCTCTGCTAG AGGCAGATGT TGCTGCTCAC CAGGATCGAA TTGACGGCAT CACAATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTC GATGCCGAAA ACATTAAAAA GAAGCAAGAG 
GCCCTTGTAG CTCGCTATGA GGCTCTCAAG GAACCCATGG TGGCCCGGAA GCAGAAGCTG 
GCAGATTCTC TTCGTCTGCA GCAGCTCTTC CGAGATGTGG AGGATGAGGA AACCTGGATT 
CGAGAAAAGG AGCCTATTGC TGCGTCCACT AACAGAGGCA AAGATCTTAT TGGAGTCCAG 
AATCTGCTAA AGAAGCACCA AGCTTTACAG GCAGAAATTG CTGGCCATGA ACCTCGCATC 
AAAGCAGTGA CACAAAAGGG CAATGCCATG GTGGAGGAAG GCCATTTTGC TGCTGAGGAT 
GTGAAGGCCA AACTGAGTGA GCTCAACCAG AAGTGGGAGG CACTGAAAGC CAAAGCCTCC 
CAGCGGAGGC AGGATCTGGA GGACTCACTA CAGGCCCAGC AGTACTTTGC CGACGCCAAT 
GAAGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATTGTGG GCAGTACCGA CTATGGGAAG 
GATGAAGACT CTGCTGAGGC TCTGCTCAAG AAGCATGAAG CTTTGATGTC CGATCTCAGT 
GCCTACGGCA GCAGCATTCA AGCTTTGCGA GAGCAGGCTC AGTCATGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGCAAGGAG CTGGTCTTGG CTCTCTATGA CTATCAAGAG 
AAGAGCCCTC GTGAGGTCAC CATGAAGAAA GGGGATATCC TCACCTTGCT CAACAGCACA 
AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGTCAGG GTTTTGTGCC AGCTGCGTAT 
GTGAAGAAGC TGGACCCCGC CCAGTCAGCC TCAAGGGAGA ACCTCCTGGA AGAACAGGGC 
AGCATTGCTC TGCGGCAGGG GCAGATCGAC AACCAGACAC GCATAACTAA GGAGGCCGGC 
AGTGTATCTC TGCGTATGAA ACAGGTGGAA GAACTGTATC AGTCTCTGCT GGAGCTGGGT 
GAGAAGAGAA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TCATGTTGTT CCGGGAAGCG 
AACGAGCTAC AGCAGTGGAT CAACGAGAAG GAAGCTGCTC TAACGAGTGA AGAGGTTGGC 
GCTGACTTGG AGCAGGTCGA GGTGCTGCAG AAGAAGTTCG ATGACTTCCA GAAGGATCTG 
AAAGCCAATG AGTCCCGGCT GAAGGACATT AACAAAGTGG CCGAGGACCT GGAGTCTGAA 
GGTCTCATGG CGGAAGAAGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG TATGATGCCC 
AGGGATGAAG CAGATTCCAA GACCGCCTCC CCATGGAAGT CTGCTCGACT GATGGTCCAC 
ACAGTGGCCA CCTTCAACTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTGCAACAG 
CTGGCTGAGG AACGTAGCCA GCTCCTGGGC AGTGCACACG AAGTACAGAG GTTCCACAGG 
GATGCGGATG AAACCAAAGA ATGGATTGAG GAGAAGAACC AGGCTCTGAA CACAGACAAC 
TATGGCCATG ATCTAGCTAG CGTCCAGGCC CTGCAGCGCA AACACGAAGG CTTCGAGAGG 
GACCTTGCAG CTCTTGGTGA CAAGGTGAAT TCCCTTGGGG AAACAGCCCA GAGGCTGATC 
CAGTCCCACC CTGAATCTGC AGAGGACTTA AAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGACCAGCC TAGGGAAGCG TGCAGACCAG CGCAAGGCCA AACTGGGTGA CTCCCATGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGAGGG 
TTGGTATCTT CAGATGAACT GGCCAAGGAT GTCACTGGAG CTGAGGCTTT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATTGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TATGCCAGCC CAGAGATCAA GGAGAAACTT 
GATATTCTTG ACCAGGAGCG CACAGACCTG GAGAAGGCCT GGGTTCAGCG CAGAATGATG 
CTGGACCACT GCCTGGAGTT GCAGCTGTTC CATCGAGACT GTGAGCAAGC AGAGAACTGG 
ATGGCTGCCC GGGAAGCCTT CCTAAACACA GAAGACAAAG GAGACTCGCT GGACAGTGTG 
GAGGCTCTGA TCAAAAAACA TGAAGACTTC GACAAAGCTA TCAATGTCCA GGAGGAGAAG 
ATAGCTGCCC TGCAGGCCTT TGCCGACCAG CTCATTGCTG TGGACCACTA TGCCAAGGGA 
GACATTGCAA ACCGACGCAA TGAGGTCCTG GACAGGTGGC GCCGCCTAAA AGCCCAGATG 
ATTGAGAAAA GGTCAAAGCT CGGAGAATCT CAAACACTTC AGCAGTTCAG CCGGGATGTA 
GATGAGATTG AAGCCTGGAT CAGTGAGAAG TTACAAACAG CCAGCGATGA GTCATACAAG 
GACCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ACCAGAAGCA CCAAGCCTTT 
GAGGCAGAAC TGCACGCCAA TGCTGACCGA ATCCGTGGAG TTATTGACAT GGGCAACTCC 
CTCATTGAGC GTGGGGCCTG TGCTGGCAGT GAGGATGCTG TCAAGGCCCG CCTGGCTGCC 
CTTGCAGACC AGTGGCAGTT CCTGGTGCAG AAGTCAGCTG AGAAGAGCCA GAAGCTGAAA 
GAGGCCAATA AGCAGCAGAA CTTCAACACC GGGATCAAAG ACTTTGACTT CTGGCTTTCT 
GAGGTGGAGG CTCTCCTGGC ATCTGAAGAC TACGGCAAAG ACCTGGCTTC CGTGAACAAC 
CTGCTCAAAA AGCATCAGCT GCTGGAGGCA GACATATCGG CCCACGAGGA TCGTCTGAAG 
GACCTGAACA GCCAGGCTGA CAGCCTGATG ACTAGCAGTG CCTTCGACAC CTCCCAAGTG 
AAAGAGAAGC GGGACACCAT CAATGGACGC TTTCAGAAGA TCAAGAGCAT GGCAACCTCC 
CGAAGAGCAA AACTGAGCGA GTCCCATCGC CTGCACCAGT TTTTCCGAGA CATGGATGAC 
GAGGAGTCCT GGATCAAGGA GAAGAAGTTG TTAGTGAGCT CTGAGGACTA TGGCAGAGAC 
CTCACTGGTG TTCAAAATCT GAGGAAGAAA CACAAGCGGC TAGAAGCCGA ACTGGCCGCA 
CACGAACCAG CCATTCAGGG TGTCCTGGAC ACGGGGAAGA AGCTGTCTGA TGACAACACC 
ATCGGGCAGG AGGAGATCCA GCAGCGTCTC GCACAGTTTG TGGAGCACTG GAAGGAACTG 
AAACAGCTAG CAGCTGCACG GGGCCAGCGG CTGGAGGAGT CCTTGGAGTA TCAGCAGTTT 
GTGGCCAACG TGGAGGAGGA GGAGGCTTGG ATCAATGAGA AGATGACCCT GGTGGCCAGC 
GAAGACTACG GGGACACTCT TGCTGCCATC CAGGGCTTAC TGAAGAAACA TGAAGCTTTT 
GAGACAGACT TCACTGTCCA CAAGGATCGA GTGAATGATG TCTGTACTAA TGGACAAGAC 
CTCATTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CAAAGATGAA GGGTCTGAAT 
GGTAAAGTGT CTGACCTGGA GAAAGCAGCA GCTCAGAGGA AAGCGAAGCT GGATGAGAAC 
TCGGCCTTCC TTCAGTTCAA TTGGAAGGCT GACGTGGTGG AGTCCTGGAT TGGTGAAAAG 
GAGAACAGCT TGAAAACAGA TGATTATGGC CGAGATCTGT CTTCTGTCCA AACTCTGCTC 
ACCAAGCAGG AGACATTTGA TGCTGGCCTG CAGGCCTTCC AGCAGGAGGG CATTGCCAAT 
ATCACTGCCC TCAAAGACCA GCTGCTAGCT GCCAAGCACA TTCAGTCGAA GGCCATCGAG 
GCCCGACATG CCTCCCTCAT GAAGAGGTGG ACCCAGCTGT TGGCCAATTC AGCTACCCGC 
AAGAAGAAGT TGCTAGAGGC CCAGAGTCAT TTCCGAAAGG TAGAAGACCT CTTCCTGACC 
TTTGCCAAAA AGGCATCGGC TTTCAACAGC TGGTTTGAGA ATGCAGAAGA GGACCTCACA 
GACCCAGTGC GCTGCAACTC TCTGGAAGAA ATCAAAGCCC TCCGAGAGGC TCATGATGCC 
TTCCGCTCAT CGCTCAGCTC TGCGCAGGCC GACTTCAACC AGCTAGCCGA GCTGGACCGT 
CAGATCAAGA GTTTCCGAGT GGCCTCCAAT CCCTACACCT GGTTCACCAT GGAGGCCCTG 
GAAGAGACGT GGAGGAACCT ACAGAAGATC ATTAAGGAGC GAGAACTGGA GCTGCAGAAG 
GAACAGCGGC GGCAGGAGGA GAATGACAAG CTACGCCAAG AGTTTGCCCA GCATGCCAAC 
GCGTTCCACC AGTGGATCCA GGAAACAAGA ACGTATCTCC TCGACGGCAT AGCATATCGT 
CGGGTCATTC GTGTCTATCA GTATGAAGTT GGGGATGATC TGTCTGGAAG GTCCTGCATG 
GTCGAAGAGT CGGGAACTCT GGAATCTCAG CTTGAAGCTA CCAAACGCAA GCACCAGGAG 
ATTCGGGCCA TGAGAAGTCA GCTGAAGAAG ATTGAGGACC TGGGGGCTGC CATGGAGGAA 
GCCCTCATCC TGGACAACAA GTACACTGAG CACAGCACTG TGGGCCTGGC CCAGCAGTGG 
GACCAGTTAG ACCAGCTGGG CATGCGCATG CAGCACAACC TGGAGCAGCA GATCCAGGCC 
AGGAACACAA CAGGAGTCAC TGAGGAGGCC CTCAAGGAGT TCAGCATGAT GTTCAAACAC 
TTCGACAAGG ACAAGTCTGG CCGGCTGAAT CATCAAGAGT TCAAATCCTG CCTTCGTTCT 
CTGGGTTACG ACCTGCCAAT GGTTGAGGAA GGAGAGCCTG ATCCTGAGTT TGAGGCCATA 
CTGGACACTG TTGATCCCAA CAGGGACGGC CACGTCTCCC TGCAAGAGTA CATGGCTTTC 
ATGATCAGCC GTGAAACCGA GAATGTCAAG TCCAGTGAAG AGATCGAGAG TGCTTTCCGG 
GCCCTCAGCT CCGAGGGCAA GCCTTATGTG ACCAAGGAGG AGCTCTACCA GAACCTGACC 
CGGGAACAAG CTGACTACTG TGTCTCCCAC ATGAAGCCCT ATGTGGATGG CAAGGGCCGC 
GAACTTCCAA CTGCCTTCGA CTACGTGGAG TTCACCCGCT CTCTCTTTGT GAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sptan1 ( XM_008761678.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008759900.1 Rattus norvegicus spectrin, alpha, non-erythrocytic 1 (Sptan1), transcript variant X6, mRNA.

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CloneID	ORa25672
Clone ID Related Accession (Same CDS sequence)	XM_008761678.2
Accession Version	XM_008761678.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008761678.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCAA GTGGGGTCAA AGTGCTGGAA ACAGCCGAGG ACATCCAGGA GAGACGACAG 
CAGGTTCTGG ATCGGTACCA CCGCTTCAAG GAGCTCTCTA CCTTGCGGCG GCAGAAACTG 
GAGGATTCCT ATCGCTTCCA GTTTTTTCAG AGAGATGCTG AGGAGCTGGA GAAGTGGATT 
CAGGAGAAGC TTCAAGTTGC CTCTGATGAG AACTACAAAG ACCCCACCAA CTTGCAGGGA 
AAGCTCCAGA AACACCAAGC CTTTGAAGCT GAAGTACAGG CCAACTCAGG AGCCATTGTG 
AAGCTGGATG AGACAGGAAA CTTGATGATT TCTGAAGGGC ACTTTGCATC TGAGACCATC 
CGGACACGTT TAATGGAGCT GCACCGCCAG TGGGAGTTGC TTTTGGAGAA GATGCGGGAG 
AAAGGAATCA AACTGCTGCA GGCACAGAAG CTGGTGCAGT ATTTGCGGGA GTGTGAGGAT 
GTAATGGACT GGATCAATGA CAAGGAAGCA ATTGTGACGT CTGAGGAGCT GGGCCAGGAT 
TTGGAGCATG TAGAGGTACT ACAGAAGAAG TTTGAAGAGT TTCAAACTGA TCTGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTAAACCAG TTTGCTGCCA AACTTATCCA GGAGCAGCAC 
CCGGAAGAGG AGCTGATCAA GACCAAGCAG GAGGAGGTGA ATGCAGCTTG GCAGCGACTG 
AAAGGCCTGG CTCTTCAAAG GCAGGGGAAG CTCTTTGGTG CTGCCGAGGT TCAGCGCTTT 
AACAGGGATG TAGATGAGAC CATTGGTTGG ATTAAGGAGA AAGAGCAGTT AATGGCCTCT 
GATGACTTTG GCAGAGACTT AGCAAGTGTT CAAGCTCTGC TTCGGAAGCA TGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAGGACAAG GTGAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCAACAGT CACACCCTCT GAGTGCCAAC CAGATCCAGG TGAAGCGAGA GGAACTAATT 
ACCAACTGGG AGCAGATCCG AACTCTGGCC GCAGAGAGAC ATGCACGGCT TGATGACTCA 
TACAGGCTTC AGCGCTTTCT TGCTGACTTC CGTGACCTCA CGAGCTGGGT GACTGAAATG 
AAAGCCCTCA TCAATGCAGA TGAACTGGCC AATGACGTGG CTGGTGCTGA GGCCCTGCTG 
GACAGGCATC AAGAGCACAA GGGTGAAATC GATGCTCATG AAGATAGCTT TAAGTCTGCA 
GATGAGTCTG GGCAGGCCCT ACTCGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGAGCA TCCTCTCTGA GGAGAGAGCT GCCCTGCTGG AGCTGTGGGA GCTTCGGAGG 
CAACAGTATG AGCAGTGCAT GGACTTGCAG CTCTTCTACC GAGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAACAGGA GGCATTCCTG CTAAATGAAG ATTTGGGTGA CTCCTTAGAC 
AGTGTGGAAG CTCTTTTGAA GAAGCATGAG GACTTTGAGA AATCTCTCAG TGCCCAGGAA 
GAAAAAATCA CAGCACTTGA TGAGTTTGCA ACCAAGCTTA TTCAGAACAA CCACTACGCA 
ATGGAAGATG TAGCCACTCG ACGAGATGCT CTCCTGAGCC GCCGCAATGC CCTCCATGAG 
CGAGCCATGC ATCGCCGGGC ACAGCTGGCC GATTCCTTCC ACCTGCAGCA GTTCTTCCGC 
GATTCCGATG AGCTCAAAAG TTGGGTCAAT GAGAAGATGA AAACGGCCAC TGATGAAGCT 
TACAAAGATC CGTCCAACCT GCAAGGGAAA GTCCAAAAGC ACCAGGCTTT TGAGGCTGAG 
CTCTCAGCCA ACCAGAGCCG TATTGATGCC CTAGAGAAAG CTGGGCAAAA ACTAATAGAT 
GTGAACCACT ATGCCAAGGA AGAAGTAGCA GCTCGGATGA ATGAGGTCAT CAGTTTGTGG 
AAGAAACTTC TAGAGGCCAC AGAACTGAAA GGAGTCAAGC TCCGAGAAGC CAACCAGCAG 
CAACAATTTA ATCGAAATGT TGAGGACATT GAGTTGTGGC TGTATGAAGT TGAAGGTCAC 
TTGGCTTCAG ATGATTATGG TAAAGACCTC ACTAATGTCC AGAACCTCCA GAAGAAGCAT 
GCTCTGCTAG AGGCAGATGT TGCTGCTCAC CAGGATCGAA TTGACGGCAT CACAATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTC GATGCCGAAA ACATTAAAAA GAAGCAAGAG 
GCCCTTGTAG CTCGCTATGA GGCTCTCAAG GAACCCATGG TGGCCCGGAA GCAGAAGCTG 
GCAGATTCTC TTCGTCTGCA GCAGCTCTTC CGAGATGTGG AGGATGAGGA AACCTGGATT 
CGAGAAAAGG AGCCTATTGC TGCGTCCACT AACAGAGGCA AAGATCTTAT TGGAGTCCAG 
AATCTGCTAA AGAAGCACCA AGCTTTACAG GCAGAAATTG CTGGCCATGA ACCTCGCATC 
AAAGCAGTGA CACAAAAGGG CAATGCCATG GTGGAGGAAG GCCATTTTGC TGCTGAGGAT 
GTGAAGGCCA AACTGAGTGA GCTCAACCAG AAGTGGGAGG CACTGAAAGC CAAAGCCTCC 
CAGCGGAGGC AGGATCTGGA GGACTCACTA CAGGCCCAGC AGTACTTTGC CGACGCCAAT 
GAAGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATTGTGG GCAGTACCGA CTATGGGAAG 
GATGAAGACT CTGCTGAGGC TCTGCTCAAG AAGCATGAAG CTTTGATGTC CGATCTCAGT 
GCCTACGGCA GCAGCATTCA AGCTTTGCGA GAGCAGGCTC AGTCATGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGCAAGGAG CTGGTCTTGG CTCTCTATGA CTATCAAGAG 
AAGAGCCCTC GTGAGGTCAC CATGAAGAAA GGGGATATCC TCACCTTGCT CAACAGCACA 
AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGTCAGG GTTTTGTGCC AGCTGCGTAT 
GTGAAGAAGC TGGACCCCGC CCAGTCAGCC TCAAGGGAGA ACCTCCTGGA AGAACAGGGC 
AGCATTGCTC TGCGGCAGGG GCAGATCGAC AACCAGTATC AGTCTCTGCT GGAGCTGGGT 
GAGAAGAGAA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TCATGTTGTT CCGGGAAGCG 
AACGAGCTAC AGCAGTGGAT CAACGAGAAG GAAGCTGCTC TAACGAGTGA AGAGGTTGGC 
GCTGACTTGG AGCAGGTCGA GGTGCTGCAG AAGAAGTTCG ATGACTTCCA GAAGGATCTG 
AAAGCCAATG AGTCCCGGCT GAAGGACATT AACAAAGTGG CCGAGGACCT GGAGTCTGAA 
GGTCTCATGG CGGAAGAAGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG TATGATGCCC 
AGGGATGAAG CAGATTCCAA GACCGCCTCC CCATGGAAGT CTGCTCGACT GATGGTCCAC 
ACAGTGGCCA CCTTCAACTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTGCAACAG 
CTGGCTGAGG AACGTAGCCA GCTCCTGGGC AGTGCACACG AAGTACAGAG GTTCCACAGG 
GATGCGGATG AAACCAAAGA ATGGATTGAG GAGAAGAACC AGGCTCTGAA CACAGACAAC 
TATGGCCATG ATCTAGCTAG CGTCCAGGCC CTGCAGCGCA AACACGAAGG CTTCGAGAGG 
GACCTTGCAG CTCTTGGTGA CAAGGTGAAT TCCCTTGGGG AAACAGCCCA GAGGCTGATC 
CAGTCCCACC CTGAATCTGC AGAGGACTTA AAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGACCAGCC TAGGGAAGCG TGCAGACCAG CGCAAGGCCA AACTGGGTGA CTCCCATGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGAGGG 
TTGGTATCTT CAGATGAACT GGCCAAGGAT GTCACTGGAG CTGAGGCTTT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATTGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TATGCCAGCC CAGAGATCAA GGAGAAACTT 
GATATTCTTG ACCAGGAGCG CACAGACCTG GAGAAGGCCT GGGTTCAGCG CAGAATGATG 
CTGGACCACT GCCTGGAGTT GCAGCTGTTC CATCGAGACT GTGAGCAAGC AGAGAACTGG 
ATGGCTGCCC GGGAAGCCTT CCTAAACACA GAAGACAAAG GAGACTCGCT GGACAGTGTG 
GAGGCTCTGA TCAAAAAACA TGAAGACTTC GACAAAGCTA TCAATGTCCA GGAGGAGAAG 
ATAGCTGCCC TGCAGGCCTT TGCCGACCAG CTCATTGCTG TGGACCACTA TGCCAAGGGA 
GACATTGCAA ACCGACGCAA TGAGGTCCTG GACAGGTGGC GCCGCCTAAA AGCCCAGATG 
ATTGAGAAAA GGTCAAAGCT CGGAGAATCT CAAACACTTC AGCAGTTCAG CCGGGATGTA 
GATGAGATTG AAGCCTGGAT CAGTGAGAAG TTACAAACAG CCAGCGATGA GTCATACAAG 
GACCCCACCA ACATCCAGAG CAAGCACCAG AAGCACCAAG CCTTTGAGGC AGAACTGCAC 
GCCAATGCTG ACCGAATCCG TGGAGTTATT GACATGGGCA ACTCCCTCAT TGAGCGTGGG 
GCCTGTGCTG GCAGTGAGGA TGCTGTCAAG GCCCGCCTGG CTGCCCTTGC AGACCAGTGG 
CAGTTCCTGG TGCAGAAGTC AGCTGAGAAG AGCCAGAAGC TGAAAGAGGC CAATAAGCAG 
CAGAACTTCA ACACCGGGAT CAAAGACTTT GACTTCTGGC TTTCTGAGGT GGAGGCTCTC 
CTGGCATCTG AAGACTACGG CAAAGACCTG GCTTCCGTGA ACAACCTGCT CAAAAAGCAT 
CAGCTGCTGG AGGCAGACAT ATCGGCCCAC GAGGATCGTC TGAAGGACCT GAACAGCCAG 
GCTGACAGCC TGATGACTAG CAGTGCCTTC GACACCTCCC AAGTGAAAGA GAAGCGGGAC 
ACCATCAATG GACGCTTTCA GAAGATCAAG AGCATGGCAA CCTCCCGAAG AGCAAAACTG 
AGCGAGTCCC ATCGCCTGCA CCAGTTTTTC CGAGACATGG ATGACGAGGA GTCCTGGATC 
AAGGAGAAGA AGTTGTTAGT GAGCTCTGAG GACTATGGCA GAGACCTCAC TGGTGTTCAA 
AATCTGAGGA AGAAACACAA GCGGCTAGAA GCCGAACTGG CCGCACACGA ACCAGCCATT 
CAGGGTGTCC TGGACACGGG GAAGAAGCTG TCTGATGACA ACACCATCGG GCAGGAGGAG 
ATCCAGCAGC GTCTCGCACA GTTTGTGGAG CACTGGAAGG AACTGAAACA GCTAGCAGCT 
GCACGGGGCC AGCGGCTGGA GGAGTCCTTG GAGTATCAGC AGTTTGTGGC CAACGTGGAG 
GAGGAGGAGG CTTGGATCAA TGAGAAGATG ACCCTGGTGG CCAGCGAAGA CTACGGGGAC 
ACTCTTGCTG CCATCCAGGG CTTACTGAAG AAACATGAAG CTTTTGAGAC AGACTTCACT 
GTCCACAAGG ATCGAGTGAA TGATGTCTGT ACTAATGGAC AAGACCTCAT TAAGAAGAAC 
AATCACCATG AGGAGAACAT CTCTTCAAAG ATGAAGGGTC TGAATGGTAA AGTGTCTGAC 
CTGGAGAAAG CAGCAGCTCA GAGGAAAGCG AAGCTGGATG AGAACTCGGC CTTCCTTCAG 
TTCAATTGGA AGGCTGACGT GGTGGAGTCC TGGATTGGTG AAAAGGAGAA CAGCTTGAAA 
ACAGATGATT ATGGCCGAGA TCTGTCTTCT GTCCAAACTC TGCTCACCAA GCAGGAGACA 
TTTGATGCTG GCCTGCAGGC CTTCCAGCAG GAGGGCATTG CCAATATCAC TGCCCTCAAA 
GACCAGCTGC TAGCTGCCAA GCACATTCAG TCGAAGGCCA TCGAGGCCCG ACATGCCTCC 
CTCATGAAGA GGTGGACCCA GCTGTTGGCC AATTCAGCTA CCCGCAAGAA GAAGTTGCTA 
GAGGCCCAGA GTCATTTCCG AAAGGTAGAA GACCTCTTCC TGACCTTTGC CAAAAAGGCA 
TCGGCTTTCA ACAGCTGGTT TGAGAATGCA GAAGAGGACC TCACAGACCC AGTGCGCTGC 
AACTCTCTGG AAGAAATCAA AGCCCTCCGA GAGGCTCATG ATGCCTTCCG CTCATCGCTC 
AGCTCTGCGC AGGCCGACTT CAACCAGCTA GCCGAGCTGG ACCGTCAGAT CAAGAGTTTC 
CGAGTGGCCT CCAATCCCTA CACCTGGTTC ACCATGGAGG CCCTGGAAGA GACGTGGAGG 
AACCTACAGA AGATCATTAA GGAGCGAGAA CTGGAGCTGC AGAAGGAACA GCGGCGGCAG 
GAGGAGAATG ACAAGCTACG CCAAGAGTTT GCCCAGCATG CCAACGCGTT CCACCAGTGG 
ATCCAGGAAA CAAGAACGTA TCTCCTCGAC GGGTCCTGCA TGGTCGAAGA GTCGGGAACT 
CTGGAATCTC AGCTTGAAGC TACCAAACGC AAGCACCAGG AGATTCGGGC CATGAGAAGT 
CAGCTGAAGA AGATTGAGGA CCTGGGGGCT GCCATGGAGG AAGCCCTCAT CCTGGACAAC 
AAGTACACTG AGCACAGCAC TGTGGGCCTG GCCCAGCAGT GGGACCAGTT AGACCAGCTG 
GGCATGCGCA TGCAGCACAA CCTGGAGCAG CAGATCCAGG CCAGGAACAC AACAGGAGTC 
ACTGAGGAGG CCCTCAAGGA GTTCAGCATG ATGTTCAAAC ACTTCGACAA GGACAAGTCT 
GGCCGGCTGA ATCATCAAGA GTTCAAATCC TGCCTTCGTT CTCTGGGTTA CGACCTGCCA 
ATGGTTGAGG AAGGAGAGCC TGATCCTGAG TTTGAGGCCA TACTGGACAC TGTTGATCCC 
AACAGGGACG GCCACGTCTC CCTGCAAGAG TACATGGCTT TCATGATCAG CCGTGAAACC 
GAGAATGTCA AGTCCAGTGA AGAGATCGAG AGTGCTTTCC GGGCCCTCAG CTCCGAGGGC 
AAGCCTTATG TGACCAAGGA GGAGCTCTAC CAGAACCTGA CCCGGGAACA AGCTGACTAC 
TGTGTCTCCC ACATGAAGCC CTATGTGGAT GGCAAGGGCC GCGAACTTCC AACTGCCTTC 
GACTACGTGG AGTTCACCCG CTCTCTCTTT GTGAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008759900.1
CDS	112..7470
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQVASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDLAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQEEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETIGWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSANQIQVKREELITNWEQIRTLAAERHARLDDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLSILSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMHRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKEEVAARMNEVISLWKKLLEATELKGVKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLSELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQGQIDNQYQSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGMMPRDEADSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAQRLIQSHPESAEDLKEKCTELNQAWTSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKEKLDILDQERTDLEKAWVQRRMMLDHCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAVDHYAKGDIANRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKEKRDTINGRFQKIKSMATSRRAKLSESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGQEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHIQSKAIEARHASLMKRWTQLLANSATRKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_008761678.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus spectrin, alpha, non-erythrocytic 1 (Sptan1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008761678.2

ORF Insert Sequence:

ATGGATCCAA GTGGGGTCAA AGTGCTGGAA ACAGCCGAGG ACATCCAGGA GAGACGACAG 
CAGGTTCTGG ATCGGTACCA CCGCTTCAAG GAGCTCTCTA CCTTGCGGCG GCAGAAACTG 
GAGGATTCCT ATCGCTTCCA GTTTTTTCAG AGAGATGCTG AGGAGCTGGA GAAGTGGATT 
CAGGAGAAGC TTCAAGTTGC CTCTGATGAG AACTACAAAG ACCCCACCAA CTTGCAGGGA 
AAGCTCCAGA AACACCAAGC CTTTGAAGCT GAAGTACAGG CCAACTCAGG AGCCATTGTG 
AAGCTGGATG AGACAGGAAA CTTGATGATT TCTGAAGGGC ACTTTGCATC TGAGACCATC 
CGGACACGTT TAATGGAGCT GCACCGCCAG TGGGAGTTGC TTTTGGAGAA GATGCGGGAG 
AAAGGAATCA AACTGCTGCA GGCACAGAAG CTGGTGCAGT ATTTGCGGGA GTGTGAGGAT 
GTAATGGACT GGATCAATGA CAAGGAAGCA ATTGTGACGT CTGAGGAGCT GGGCCAGGAT 
TTGGAGCATG TAGAGGTACT ACAGAAGAAG TTTGAAGAGT TTCAAACTGA TCTGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTAAACCAG TTTGCTGCCA AACTTATCCA GGAGCAGCAC 
CCGGAAGAGG AGCTGATCAA GACCAAGCAG GAGGAGGTGA ATGCAGCTTG GCAGCGACTG 
AAAGGCCTGG CTCTTCAAAG GCAGGGGAAG CTCTTTGGTG CTGCCGAGGT TCAGCGCTTT 
AACAGGGATG TAGATGAGAC CATTGGTTGG ATTAAGGAGA AAGAGCAGTT AATGGCCTCT 
GATGACTTTG GCAGAGACTT AGCAAGTGTT CAAGCTCTGC TTCGGAAGCA TGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAGGACAAG GTGAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCAACAGT CACACCCTCT GAGTGCCAAC CAGATCCAGG TGAAGCGAGA GGAACTAATT 
ACCAACTGGG AGCAGATCCG AACTCTGGCC GCAGAGAGAC ATGCACGGCT TGATGACTCA 
TACAGGCTTC AGCGCTTTCT TGCTGACTTC CGTGACCTCA CGAGCTGGGT GACTGAAATG 
AAAGCCCTCA TCAATGCAGA TGAACTGGCC AATGACGTGG CTGGTGCTGA GGCCCTGCTG 
GACAGGCATC AAGAGCACAA GGGTGAAATC GATGCTCATG AAGATAGCTT TAAGTCTGCA 
GATGAGTCTG GGCAGGCCCT ACTCGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGAGCA TCCTCTCTGA GGAGAGAGCT GCCCTGCTGG AGCTGTGGGA GCTTCGGAGG 
CAACAGTATG AGCAGTGCAT GGACTTGCAG CTCTTCTACC GAGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAACAGGA GGCATTCCTG CTAAATGAAG ATTTGGGTGA CTCCTTAGAC 
AGTGTGGAAG CTCTTTTGAA GAAGCATGAG GACTTTGAGA AATCTCTCAG TGCCCAGGAA 
GAAAAAATCA CAGCACTTGA TGAGTTTGCA ACCAAGCTTA TTCAGAACAA CCACTACGCA 
ATGGAAGATG TAGCCACTCG ACGAGATGCT CTCCTGAGCC GCCGCAATGC CCTCCATGAG 
CGAGCCATGC ATCGCCGGGC ACAGCTGGCC GATTCCTTCC ACCTGCAGCA GTTCTTCCGC 
GATTCCGATG AGCTCAAAAG TTGGGTCAAT GAGAAGATGA AAACGGCCAC TGATGAAGCT 
TACAAAGATC CGTCCAACCT GCAAGGGAAA GTCCAAAAGC ACCAGGCTTT TGAGGCTGAG 
CTCTCAGCCA ACCAGAGCCG TATTGATGCC CTAGAGAAAG CTGGGCAAAA ACTAATAGAT 
GTGAACCACT ATGCCAAGGA AGAAGTAGCA GCTCGGATGA ATGAGGTCAT CAGTTTGTGG 
AAGAAACTTC TAGAGGCCAC AGAACTGAAA GGAGTCAAGC TCCGAGAAGC CAACCAGCAG 
CAACAATTTA ATCGAAATGT TGAGGACATT GAGTTGTGGC TGTATGAAGT TGAAGGTCAC 
TTGGCTTCAG ATGATTATGG TAAAGACCTC ACTAATGTCC AGAACCTCCA GAAGAAGCAT 
GCTCTGCTAG AGGCAGATGT TGCTGCTCAC CAGGATCGAA TTGACGGCAT CACAATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTC GATGCCGAAA ACATTAAAAA GAAGCAAGAG 
GCCCTTGTAG CTCGCTATGA GGCTCTCAAG GAACCCATGG TGGCCCGGAA GCAGAAGCTG 
GCAGATTCTC TTCGTCTGCA GCAGCTCTTC CGAGATGTGG AGGATGAGGA AACCTGGATT 
CGAGAAAAGG AGCCTATTGC TGCGTCCACT AACAGAGGCA AAGATCTTAT TGGAGTCCAG 
AATCTGCTAA AGAAGCACCA AGCTTTACAG GCAGAAATTG CTGGCCATGA ACCTCGCATC 
AAAGCAGTGA CACAAAAGGG CAATGCCATG GTGGAGGAAG GCCATTTTGC TGCTGAGGAT 
GTGAAGGCCA AACTGAGTGA GCTCAACCAG AAGTGGGAGG CACTGAAAGC CAAAGCCTCC 
CAGCGGAGGC AGGATCTGGA GGACTCACTA CAGGCCCAGC AGTACTTTGC CGACGCCAAT 
GAAGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATTGTGG GCAGTACCGA CTATGGGAAG 
GATGAAGACT CTGCTGAGGC TCTGCTCAAG AAGCATGAAG CTTTGATGTC CGATCTCAGT 
GCCTACGGCA GCAGCATTCA AGCTTTGCGA GAGCAGGCTC AGTCATGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGCAAGGAG CTGGTCTTGG CTCTCTATGA CTATCAAGAG 
AAGAGCCCTC GTGAGGTCAC CATGAAGAAA GGGGATATCC TCACCTTGCT CAACAGCACA 
AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGTCAGG GTTTTGTGCC AGCTGCGTAT 
GTGAAGAAGC TGGACCCCGC CCAGTCAGCC TCAAGGGAGA ACCTCCTGGA AGAACAGGGC 
AGCATTGCTC TGCGGCAGGG GCAGATCGAC AACCAGTATC AGTCTCTGCT GGAGCTGGGT 
GAGAAGAGAA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TCATGTTGTT CCGGGAAGCG 
AACGAGCTAC AGCAGTGGAT CAACGAGAAG GAAGCTGCTC TAACGAGTGA AGAGGTTGGC 
GCTGACTTGG AGCAGGTCGA GGTGCTGCAG AAGAAGTTCG ATGACTTCCA GAAGGATCTG 
AAAGCCAATG AGTCCCGGCT GAAGGACATT AACAAAGTGG CCGAGGACCT GGAGTCTGAA 
GGTCTCATGG CGGAAGAAGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG TATGATGCCC 
AGGGATGAAG CAGATTCCAA GACCGCCTCC CCATGGAAGT CTGCTCGACT GATGGTCCAC 
ACAGTGGCCA CCTTCAACTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTGCAACAG 
CTGGCTGAGG AACGTAGCCA GCTCCTGGGC AGTGCACACG AAGTACAGAG GTTCCACAGG 
GATGCGGATG AAACCAAAGA ATGGATTGAG GAGAAGAACC AGGCTCTGAA CACAGACAAC 
TATGGCCATG ATCTAGCTAG CGTCCAGGCC CTGCAGCGCA AACACGAAGG CTTCGAGAGG 
GACCTTGCAG CTCTTGGTGA CAAGGTGAAT TCCCTTGGGG AAACAGCCCA GAGGCTGATC 
CAGTCCCACC CTGAATCTGC AGAGGACTTA AAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGACCAGCC TAGGGAAGCG TGCAGACCAG CGCAAGGCCA AACTGGGTGA CTCCCATGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGAGGG 
TTGGTATCTT CAGATGAACT GGCCAAGGAT GTCACTGGAG CTGAGGCTTT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATTGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TATGCCAGCC CAGAGATCAA GGAGAAACTT 
GATATTCTTG ACCAGGAGCG CACAGACCTG GAGAAGGCCT GGGTTCAGCG CAGAATGATG 
CTGGACCACT GCCTGGAGTT GCAGCTGTTC CATCGAGACT GTGAGCAAGC AGAGAACTGG 
ATGGCTGCCC GGGAAGCCTT CCTAAACACA GAAGACAAAG GAGACTCGCT GGACAGTGTG 
GAGGCTCTGA TCAAAAAACA TGAAGACTTC GACAAAGCTA TCAATGTCCA GGAGGAGAAG 
ATAGCTGCCC TGCAGGCCTT TGCCGACCAG CTCATTGCTG TGGACCACTA TGCCAAGGGA 
GACATTGCAA ACCGACGCAA TGAGGTCCTG GACAGGTGGC GCCGCCTAAA AGCCCAGATG 
ATTGAGAAAA GGTCAAAGCT CGGAGAATCT CAAACACTTC AGCAGTTCAG CCGGGATGTA 
GATGAGATTG AAGCCTGGAT CAGTGAGAAG TTACAAACAG CCAGCGATGA GTCATACAAG 
GACCCCACCA ACATCCAGAG CAAGCACCAG AAGCACCAAG CCTTTGAGGC AGAACTGCAC 
GCCAATGCTG ACCGAATCCG TGGAGTTATT GACATGGGCA ACTCCCTCAT TGAGCGTGGG 
GCCTGTGCTG GCAGTGAGGA TGCTGTCAAG GCCCGCCTGG CTGCCCTTGC AGACCAGTGG 
CAGTTCCTGG TGCAGAAGTC AGCTGAGAAG AGCCAGAAGC TGAAAGAGGC CAATAAGCAG 
CAGAACTTCA ACACCGGGAT CAAAGACTTT GACTTCTGGC TTTCTGAGGT GGAGGCTCTC 
CTGGCATCTG AAGACTACGG CAAAGACCTG GCTTCCGTGA ACAACCTGCT CAAAAAGCAT 
CAGCTGCTGG AGGCAGACAT ATCGGCCCAC GAGGATCGTC TGAAGGACCT GAACAGCCAG 
GCTGACAGCC TGATGACTAG CAGTGCCTTC GACACCTCCC AAGTGAAAGA GAAGCGGGAC 
ACCATCAATG GACGCTTTCA GAAGATCAAG AGCATGGCAA CCTCCCGAAG AGCAAAACTG 
AGCGAGTCCC ATCGCCTGCA CCAGTTTTTC CGAGACATGG ATGACGAGGA GTCCTGGATC 
AAGGAGAAGA AGTTGTTAGT GAGCTCTGAG GACTATGGCA GAGACCTCAC TGGTGTTCAA 
AATCTGAGGA AGAAACACAA GCGGCTAGAA GCCGAACTGG CCGCACACGA ACCAGCCATT 
CAGGGTGTCC TGGACACGGG GAAGAAGCTG TCTGATGACA ACACCATCGG GCAGGAGGAG 
ATCCAGCAGC GTCTCGCACA GTTTGTGGAG CACTGGAAGG AACTGAAACA GCTAGCAGCT 
GCACGGGGCC AGCGGCTGGA GGAGTCCTTG GAGTATCAGC AGTTTGTGGC CAACGTGGAG 
GAGGAGGAGG CTTGGATCAA TGAGAAGATG ACCCTGGTGG CCAGCGAAGA CTACGGGGAC 
ACTCTTGCTG CCATCCAGGG CTTACTGAAG AAACATGAAG CTTTTGAGAC AGACTTCACT 
GTCCACAAGG ATCGAGTGAA TGATGTCTGT ACTAATGGAC AAGACCTCAT TAAGAAGAAC 
AATCACCATG AGGAGAACAT CTCTTCAAAG ATGAAGGGTC TGAATGGTAA AGTGTCTGAC 
CTGGAGAAAG CAGCAGCTCA GAGGAAAGCG AAGCTGGATG AGAACTCGGC CTTCCTTCAG 
TTCAATTGGA AGGCTGACGT GGTGGAGTCC TGGATTGGTG AAAAGGAGAA CAGCTTGAAA 
ACAGATGATT ATGGCCGAGA TCTGTCTTCT GTCCAAACTC TGCTCACCAA GCAGGAGACA 
TTTGATGCTG GCCTGCAGGC CTTCCAGCAG GAGGGCATTG CCAATATCAC TGCCCTCAAA 
GACCAGCTGC TAGCTGCCAA GCACATTCAG TCGAAGGCCA TCGAGGCCCG ACATGCCTCC 
CTCATGAAGA GGTGGACCCA GCTGTTGGCC AATTCAGCTA CCCGCAAGAA GAAGTTGCTA 
GAGGCCCAGA GTCATTTCCG AAAGGTAGAA GACCTCTTCC TGACCTTTGC CAAAAAGGCA 
TCGGCTTTCA ACAGCTGGTT TGAGAATGCA GAAGAGGACC TCACAGACCC AGTGCGCTGC 
AACTCTCTGG AAGAAATCAA AGCCCTCCGA GAGGCTCATG ATGCCTTCCG CTCATCGCTC 
AGCTCTGCGC AGGCCGACTT CAACCAGCTA GCCGAGCTGG ACCGTCAGAT CAAGAGTTTC 
CGAGTGGCCT CCAATCCCTA CACCTGGTTC ACCATGGAGG CCCTGGAAGA GACGTGGAGG 
AACCTACAGA AGATCATTAA GGAGCGAGAA CTGGAGCTGC AGAAGGAACA GCGGCGGCAG 
GAGGAGAATG ACAAGCTACG CCAAGAGTTT GCCCAGCATG CCAACGCGTT CCACCAGTGG 
ATCCAGGAAA CAAGAACGTA TCTCCTCGAC GGGTCCTGCA TGGTCGAAGA GTCGGGAACT 
CTGGAATCTC AGCTTGAAGC TACCAAACGC AAGCACCAGG AGATTCGGGC CATGAGAAGT 
CAGCTGAAGA AGATTGAGGA CCTGGGGGCT GCCATGGAGG AAGCCCTCAT CCTGGACAAC 
AAGTACACTG AGCACAGCAC TGTGGGCCTG GCCCAGCAGT GGGACCAGTT AGACCAGCTG 
GGCATGCGCA TGCAGCACAA CCTGGAGCAG CAGATCCAGG CCAGGAACAC AACAGGAGTC 
ACTGAGGAGG CCCTCAAGGA GTTCAGCATG ATGTTCAAAC ACTTCGACAA GGACAAGTCT 
GGCCGGCTGA ATCATCAAGA GTTCAAATCC TGCCTTCGTT CTCTGGGTTA CGACCTGCCA 
ATGGTTGAGG AAGGAGAGCC TGATCCTGAG TTTGAGGCCA TACTGGACAC TGTTGATCCC 
AACAGGGACG GCCACGTCTC CCTGCAAGAG TACATGGCTT TCATGATCAG CCGTGAAACC 
GAGAATGTCA AGTCCAGTGA AGAGATCGAG AGTGCTTTCC GGGCCCTCAG CTCCGAGGGC 
AAGCCTTATG TGACCAAGGA GGAGCTCTAC CAGAACCTGA CCCGGGAACA AGCTGACTAC 
TGTGTCTCCC ACATGAAGCC CTATGTGGAT GGCAAGGGCC GCGAACTTCC AACTGCCTTC 
GACTACGTGG AGTTCACCCG CTCTCTCTTT GTGAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sptan1 ( XM_008761674.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008759896.1 Rattus norvegicus spectrin, alpha, non-erythrocytic 1 (Sptan1), transcript variant X2, mRNA.

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CloneID	ORa43561
Clone ID Related Accession (Same CDS sequence)	XM_008761674.2
Accession Version	XM_008761674.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7482bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008761674.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCAA GTGGGGTCAA AGTGCTGGAA ACAGCCGAGG ACATCCAGGA GAGACGACAG 
CAGGTTCTGG ATCGGTACCA CCGCTTCAAG GAGCTCTCTA CCTTGCGGCG GCAGAAACTG 
GAGGATTCCT ATCGCTTCCA GTTTTTTCAG AGAGATGCTG AGGAGCTGGA GAAGTGGATT 
CAGGAGAAGC TTCAAGTTGC CTCTGATGAG AACTACAAAG ACCCCACCAA CTTGCAGGGA 
AAGCTCCAGA AACACCAAGC CTTTGAAGCT GAAGTACAGG CCAACTCAGG AGCCATTGTG 
AAGCTGGATG AGACAGGAAA CTTGATGATT TCTGAAGGGC ACTTTGCATC TGAGACCATC 
CGGACACGTT TAATGGAGCT GCACCGCCAG TGGGAGTTGC TTTTGGAGAA GATGCGGGAG 
AAAGGAATCA AACTGCTGCA GGCACAGAAG CTGGTGCAGT ATTTGCGGGA GTGTGAGGAT 
GTAATGGACT GGATCAATGA CAAGGAAGCA ATTGTGACGT CTGAGGAGCT GGGCCAGGAT 
TTGGAGCATG TAGAGGTACT ACAGAAGAAG TTTGAAGAGT TTCAAACTGA TCTGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTAAACCAG TTTGCTGCCA AACTTATCCA GGAGCAGCAC 
CCGGAAGAGG AGCTGATCAA GACCAAGCAG GAGGAGGTGA ATGCAGCTTG GCAGCGACTG 
AAAGGCCTGG CTCTTCAAAG GCAGGGGAAG CTCTTTGGTG CTGCCGAGGT TCAGCGCTTT 
AACAGGGATG TAGATGAGAC CATTGGTTGG ATTAAGGAGA AAGAGCAGTT AATGGCCTCT 
GATGACTTTG GCAGAGACTT AGCAAGTGTT CAAGCTCTGC TTCGGAAGCA TGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAGGACAAG GTGAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCAACAGT CACACCCTCT GAGTGCCAAC CAGATCCAGG TGAAGCGAGA GGAACTAATT 
ACCAACTGGG AGCAGATCCG AACTCTGGCC GCAGAGAGAC ATGCACGGCT TGATGACTCA 
TACAGGCTTC AGCGCTTTCT TGCTGACTTC CGTGACCTCA CGAGCTGGGT GACTGAAATG 
AAAGCCCTCA TCAATGCAGA TGAACTGGCC AATGACGTGG CTGGTGCTGA GGCCCTGCTG 
GACAGGCATC AAGAGCACAA GGGTGAAATC GATGCTCATG AAGATAGCTT TAAGTCTGCA 
GATGAGTCTG GGCAGGCCCT ACTCGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGAGCA TCCTCTCTGA GGAGAGAGCT GCCCTGCTGG AGCTGTGGGA GCTTCGGAGG 
CAACAGTATG AGCAGTGCAT GGACTTGCAG CTCTTCTACC GAGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAACAGGA GGCATTCCTG CTAAATGAAG ATTTGGGTGA CTCCTTAGAC 
AGTGTGGAAG CTCTTTTGAA GAAGCATGAG GACTTTGAGA AATCTCTCAG TGCCCAGGAA 
GAAAAAATCA CAGCACTTGA TGAGTTTGCA ACCAAGCTTA TTCAGAACAA CCACTACGCA 
ATGGAAGATG TAGCCACTCG ACGAGATGCT CTCCTGAGCC GCCGCAATGC CCTCCATGAG 
CGAGCCATGC ATCGCCGGGC ACAGCTGGCC GATTCCTTCC ACCTGCAGCA GTTCTTCCGC 
GATTCCGATG AGCTCAAAAG TTGGGTCAAT GAGAAGATGA AAACGGCCAC TGATGAAGCT 
TACAAAGATC CGTCCAACCT GCAAGGGAAA GTCCAAAAGC ACCAGGCTTT TGAGGCTGAG 
CTCTCAGCCA ACCAGAGCCG TATTGATGCC CTAGAGAAAG CTGGGCAAAA ACTAATAGAT 
GTGAACCACT ATGCCAAGGA AGAAGTAGCA GCTCGGATGA ATGAGGTCAT CAGTTTGTGG 
AAGAAACTTC TAGAGGCCAC AGAACTGAAA GGAGTCAAGC TCCGAGAAGC CAACCAGCAG 
CAACAATTTA ATCGAAATGT TGAGGACATT GAGTTGTGGC TGTATGAAGT TGAAGGTCAC 
TTGGCTTCAG ATGATTATGG TAAAGACCTC ACTAATGTCC AGAACCTCCA GAAGAAGCAT 
GCTCTGCTAG AGGCAGATGT TGCTGCTCAC CAGGATCGAA TTGACGGCAT CACAATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTC GATGCCGAAA ACATTAAAAA GAAGCAAGAG 
GCCCTTGTAG CTCGCTATGA GGCTCTCAAG GAACCCATGG TGGCCCGGAA GCAGAAGCTG 
GCAGATTCTC TTCGTCTGCA GCAGCTCTTC CGAGATGTGG AGGATGAGGA AACCTGGATT 
CGAGAAAAGG AGCCTATTGC TGCGTCCACT AACAGAGGCA AAGATCTTAT TGGAGTCCAG 
AATCTGCTAA AGAAGCACCA AGCTTTACAG GCAGAAATTG CTGGCCATGA ACCTCGCATC 
AAAGCAGTGA CACAAAAGGG CAATGCCATG GTGGAGGAAG GCCATTTTGC TGCTGAGGAT 
GTGAAGGCCA AACTGAGTGA GCTCAACCAG AAGTGGGAGG CACTGAAAGC CAAAGCCTCC 
CAGCGGAGGC AGGATCTGGA GGACTCACTA CAGGCCCAGC AGTACTTTGC CGACGCCAAT 
GAAGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATTGTGG GCAGTACCGA CTATGGGAAG 
GATGAAGACT CTGCTGAGGC TCTGCTCAAG AAGCATGAAG CTTTGATGTC CGATCTCAGT 
GCCTACGGCA GCAGCATTCA AGCTTTGCGA GAGCAGGCTC AGTCATGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGCAAGGAG CTGGTCTTGG CTCTCTATGA CTATCAAGAG 
AAGAGCCCTC GTGAGGTCAC CATGAAGAAA GGGGATATCC TCACCTTGCT CAACAGCACA 
AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGTCAGG GTTTTGTGCC AGCTGCGTAT 
GTGAAGAAGC TGGACCCCGC CCAGTCAGCC TCAAGGGAGA ACCTCCTGGA AGAACAGGGC 
AGCATTGCTC TGCGGCAGGG GCAGATCGAC AACCAGACAC GCATAACTAA GGAGGCCGGC 
AGTGTATCTC TGCGTATGAA ACAGGTGGAA GAACTGTATC AGTCTCTGCT GGAGCTGGGT 
GAGAAGAGAA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TCATGTTGTT CCGGGAAGCG 
AACGAGCTAC AGCAGTGGAT CAACGAGAAG GAAGCTGCTC TAACGAGTGA AGAGGTTGGC 
GCTGACTTGG AGCAGGTCGA GGTGCTGCAG AAGAAGTTCG ATGACTTCCA GAAGGATCTG 
AAAGCCAATG AGTCCCGGCT GAAGGACATT AACAAAGTGG CCGAGGACCT GGAGTCTGAA 
GGTCTCATGG CGGAAGAAGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG TATGATGCCC 
AGGGATGAAG CAGATTCCAA GACCGCCTCC CCATGGAAGT CTGCTCGACT GATGGTCCAC 
ACAGTGGCCA CCTTCAACTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTGCAACAG 
CTGGCTGAGG AACGTAGCCA GCTCCTGGGC AGTGCACACG AAGTACAGAG GTTCCACAGG 
GATGCGGATG AAACCAAAGA ATGGATTGAG GAGAAGAACC AGGCTCTGAA CACAGACAAC 
TATGGCCATG ATCTAGCTAG CGTCCAGGCC CTGCAGCGCA AACACGAAGG CTTCGAGAGG 
GACCTTGCAG CTCTTGGTGA CAAGGTGAAT TCCCTTGGGG AAACAGCCCA GAGGCTGATC 
CAGTCCCACC CTGAATCTGC AGAGGACTTA AAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGACCAGCC TAGGGAAGCG TGCAGACCAG CGCAAGGCCA AACTGGGTGA CTCCCATGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGAGGG 
TTGGTATCTT CAGATGAACT GGCCAAGGAT GTCACTGGAG CTGAGGCTTT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATTGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TATGCCAGCC CAGAGATCAA GGAGAAACTT 
GATATTCTTG ACCAGGAGCG CACAGACCTG GAGAAGGCCT GGGTTCAGCG CAGAATGATG 
CTGGACCACT GCCTGGAGTT GCAGCTGTTC CATCGAGACT GTGAGCAAGC AGAGAACTGG 
ATGGCTGCCC GGGAAGCCTT CCTAAACACA GAAGACAAAG GAGACTCGCT GGACAGTGTG 
GAGGCTCTGA TCAAAAAACA TGAAGACTTC GACAAAGCTA TCAATGTCCA GGAGGAGAAG 
ATAGCTGCCC TGCAGGCCTT TGCCGACCAG CTCATTGCTG TGGACCACTA TGCCAAGGGA 
GACATTGCAA ACCGACGCAA TGAGGTCCTG GACAGGTGGC GCCGCCTAAA AGCCCAGATG 
ATTGAGAAAA GGTCAAAGCT CGGAGAATCT CAAACACTTC AGCAGTTCAG CCGGGATGTA 
GATGAGATTG AAGCCTGGAT CAGTGAGAAG TTACAAACAG CCAGCGATGA GTCATACAAG 
GACCCCACCA ACATCCAGAG CAAGCACCAG AAGCACCAAG CCTTTGAGGC AGAACTGCAC 
GCCAATGCTG ACCGAATCCG TGGAGTTATT GACATGGGCA ACTCCCTCAT TGAGCGTGGG 
GCCTGTGCTG GCAGTGAGGA TGCTGTCAAG GCCCGCCTGG CTGCCCTTGC AGACCAGTGG 
CAGTTCCTGG TGCAGAAGTC AGCTGAGAAG AGCCAGAAGC TGAAAGAGGC CAATAAGCAG 
CAGAACTTCA ACACCGGGAT CAAAGACTTT GACTTCTGGC TTTCTGAGGT GGAGGCTCTC 
CTGGCATCTG AAGACTACGG CAAAGACCTG GCTTCCGTGA ACAACCTGCT CAAAAAGCAT 
CAGCTGCTGG AGGCAGACAT ATCGGCCCAC GAGGATCGTC TGAAGGACCT GAACAGCCAG 
GCTGACAGCC TGATGACTAG CAGTGCCTTC GACACCTCCC AAGTGAAAGA GAAGCGGGAC 
ACCATCAATG GACGCTTTCA GAAGATCAAG AGCATGGCAA CCTCCCGAAG AGCAAAACTG 
AGCGAGTCCC ATCGCCTGCA CCAGTTTTTC CGAGACATGG ATGACGAGGA GTCCTGGATC 
AAGGAGAAGA AGTTGTTAGT GAGCTCTGAG GACTATGGCA GAGACCTCAC TGGTGTTCAA 
AATCTGAGGA AGAAACACAA GCGGCTAGAA GCCGAACTGG CCGCACACGA ACCAGCCATT 
CAGGGTGTCC TGGACACGGG GAAGAAGCTG TCTGATGACA ACACCATCGG GCAGGAGGAG 
ATCCAGCAGC GTCTCGCACA GTTTGTGGAG CACTGGAAGG AACTGAAACA GCTAGCAGCT 
GCACGGGGCC AGCGGCTGGA GGAGTCCTTG GAGTATCAGC AGTTTGTGGC CAACGTGGAG 
GAGGAGGAGG CTTGGATCAA TGAGAAGATG ACCCTGGTGG CCAGCGAAGA CTACGGGGAC 
ACTCTTGCTG CCATCCAGGG CTTACTGAAG AAACATGAAG CTTTTGAGAC AGACTTCACT 
GTCCACAAGG ATCGAGTGAA TGATGTCTGT ACTAATGGAC AAGACCTCAT TAAGAAGAAC 
AATCACCATG AGGAGAACAT CTCTTCAAAG ATGAAGGGTC TGAATGGTAA AGTGTCTGAC 
CTGGAGAAAG CAGCAGCTCA GAGGAAAGCG AAGCTGGATG AGAACTCGGC CTTCCTTCAG 
TTCAATTGGA AGGCTGACGT GGTGGAGTCC TGGATTGGTG AAAAGGAGAA CAGCTTGAAA 
ACAGATGATT ATGGCCGAGA TCTGTCTTCT GTCCAAACTC TGCTCACCAA GCAGGAGACA 
TTTGATGCTG GCCTGCAGGC CTTCCAGCAG GAGGGCATTG CCAATATCAC TGCCCTCAAA 
GACCAGCTGC TAGCTGCCAA GCACATTCAG TCGAAGGCCA TCGAGGCCCG ACATGCCTCC 
CTCATGAAGA GGTGGACCCA GCTGTTGGCC AATTCAGCTA CCCGCAAGAA GAAGTTGCTA 
GAGGCCCAGA GTCATTTCCG AAAGGTAGAA GACCTCTTCC TGACCTTTGC CAAAAAGGCA 
TCGGCTTTCA ACAGCTGGTT TGAGAATGCA GAAGAGGACC TCACAGACCC AGTGCGCTGC 
AACTCTCTGG AAGAAATCAA AGCCCTCCGA GAGGCTCATG ATGCCTTCCG CTCATCGCTC 
AGCTCTGCGC AGGCCGACTT CAACCAGCTA GCCGAGCTGG ACCGTCAGAT CAAGAGTTTC 
CGAGTGGCCT CCAATCCCTA CACCTGGTTC ACCATGGAGG CCCTGGAAGA GACGTGGAGG 
AACCTACAGA AGATCATTAA GGAGCGAGAA CTGGAGCTGC AGAAGGAACA GCGGCGGCAG 
GAGGAGAATG ACAAGCTACG CCAAGAGTTT GCCCAGCATG CCAACGCGTT CCACCAGTGG 
ATCCAGGAAA CAAGAACGTA TCTCCTCGAC GGCATAGCAT ATCGTCGGGT CATTCGTGTC 
TATCAGTATG AAGTTGGGGA TGATCTGTCT GGAAGGTCCT GCATGGTCGA AGAGTCGGGA 
ACTCTGGAAT CTCAGCTTGA AGCTACCAAA CGCAAGCACC AGGAGATTCG GGCCATGAGA 
AGTCAGCTGA AGAAGATTGA GGACCTGGGG GCTGCCATGG AGGAAGCCCT CATCCTGGAC 
AACAAGTACA CTGAGCACAG CACTGTGGGC CTGGCCCAGC AGTGGGACCA GTTAGACCAG 
CTGGGCATGC GCATGCAGCA CAACCTGGAG CAGCAGATCC AGGCCAGGAA CACAACAGGA 
GTCACTGAGG AGGCCCTCAA GGAGTTCAGC ATGATGTTCA AACACTTCGA CAAGGACAAG 
TCTGGCCGGC TGAATCATCA AGAGTTCAAA TCCTGCCTTC GTTCTCTGGG TTACGACCTG 
CCAATGGTTG AGGAAGGAGA GCCTGATCCT GAGTTTGAGG CCATACTGGA CACTGTTGAT 
CCCAACAGGG ACGGCCACGT CTCCCTGCAA GAGTACATGG CTTTCATGAT CAGCCGTGAA 
ACCGAGAATG TCAAGTCCAG TGAAGAGATC GAGAGTGCTT TCCGGGCCCT CAGCTCCGAG 
GGCAAGCCTT ATGTGACCAA GGAGGAGCTC TACCAGAACC TGACCCGGGA ACAAGCTGAC 
TACTGTGTCT CCCACATGAA GCCCTATGTG GATGGCAAGG GCCGCGAACT TCCAACTGCC 
TTCGACTACG TGGAGTTCAC CCGCTCTCTC TTTGTGAATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008759896.1
CDS	112..7593
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQVASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDLAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQEEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETIGWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSANQIQVKREELITNWEQIRTLAAERHARLDDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLSILSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMHRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKEEVAARMNEVISLWKKLLEATELKGVKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLSELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQGQIDNQTRITKEAGSVSLRMKQVEELYQSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGMMPRDEADSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAQRLIQSHPESAEDLKEKCTELNQAWTSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKEKLDILDQERTDLEKAWVQRRMMLDHCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAVDHYAKGDIANRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKEKRDTINGRFQKIKSMATSRRAKLSESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGQEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHIQSKAIEARHASLMKRWTQLLANSATRKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGIAYRRVIRVYQYEVGDDLSGRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_008761674.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus spectrin, alpha, non-erythrocytic 1 (Sptan1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008761674.2

ORF Insert Sequence:

ATGGATCCAA GTGGGGTCAA AGTGCTGGAA ACAGCCGAGG ACATCCAGGA GAGACGACAG 
CAGGTTCTGG ATCGGTACCA CCGCTTCAAG GAGCTCTCTA CCTTGCGGCG GCAGAAACTG 
GAGGATTCCT ATCGCTTCCA GTTTTTTCAG AGAGATGCTG AGGAGCTGGA GAAGTGGATT 
CAGGAGAAGC TTCAAGTTGC CTCTGATGAG AACTACAAAG ACCCCACCAA CTTGCAGGGA 
AAGCTCCAGA AACACCAAGC CTTTGAAGCT GAAGTACAGG CCAACTCAGG AGCCATTGTG 
AAGCTGGATG AGACAGGAAA CTTGATGATT TCTGAAGGGC ACTTTGCATC TGAGACCATC 
CGGACACGTT TAATGGAGCT GCACCGCCAG TGGGAGTTGC TTTTGGAGAA GATGCGGGAG 
AAAGGAATCA AACTGCTGCA GGCACAGAAG CTGGTGCAGT ATTTGCGGGA GTGTGAGGAT 
GTAATGGACT GGATCAATGA CAAGGAAGCA ATTGTGACGT CTGAGGAGCT GGGCCAGGAT 
TTGGAGCATG TAGAGGTACT ACAGAAGAAG TTTGAAGAGT TTCAAACTGA TCTGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTAAACCAG TTTGCTGCCA AACTTATCCA GGAGCAGCAC 
CCGGAAGAGG AGCTGATCAA GACCAAGCAG GAGGAGGTGA ATGCAGCTTG GCAGCGACTG 
AAAGGCCTGG CTCTTCAAAG GCAGGGGAAG CTCTTTGGTG CTGCCGAGGT TCAGCGCTTT 
AACAGGGATG TAGATGAGAC CATTGGTTGG ATTAAGGAGA AAGAGCAGTT AATGGCCTCT 
GATGACTTTG GCAGAGACTT AGCAAGTGTT CAAGCTCTGC TTCGGAAGCA TGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAGGACAAG GTGAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCAACAGT CACACCCTCT GAGTGCCAAC CAGATCCAGG TGAAGCGAGA GGAACTAATT 
ACCAACTGGG AGCAGATCCG AACTCTGGCC GCAGAGAGAC ATGCACGGCT TGATGACTCA 
TACAGGCTTC AGCGCTTTCT TGCTGACTTC CGTGACCTCA CGAGCTGGGT GACTGAAATG 
AAAGCCCTCA TCAATGCAGA TGAACTGGCC AATGACGTGG CTGGTGCTGA GGCCCTGCTG 
GACAGGCATC AAGAGCACAA GGGTGAAATC GATGCTCATG AAGATAGCTT TAAGTCTGCA 
GATGAGTCTG GGCAGGCCCT ACTCGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGAGCA TCCTCTCTGA GGAGAGAGCT GCCCTGCTGG AGCTGTGGGA GCTTCGGAGG 
CAACAGTATG AGCAGTGCAT GGACTTGCAG CTCTTCTACC GAGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAACAGGA GGCATTCCTG CTAAATGAAG ATTTGGGTGA CTCCTTAGAC 
AGTGTGGAAG CTCTTTTGAA GAAGCATGAG GACTTTGAGA AATCTCTCAG TGCCCAGGAA 
GAAAAAATCA CAGCACTTGA TGAGTTTGCA ACCAAGCTTA TTCAGAACAA CCACTACGCA 
ATGGAAGATG TAGCCACTCG ACGAGATGCT CTCCTGAGCC GCCGCAATGC CCTCCATGAG 
CGAGCCATGC ATCGCCGGGC ACAGCTGGCC GATTCCTTCC ACCTGCAGCA GTTCTTCCGC 
GATTCCGATG AGCTCAAAAG TTGGGTCAAT GAGAAGATGA AAACGGCCAC TGATGAAGCT 
TACAAAGATC CGTCCAACCT GCAAGGGAAA GTCCAAAAGC ACCAGGCTTT TGAGGCTGAG 
CTCTCAGCCA ACCAGAGCCG TATTGATGCC CTAGAGAAAG CTGGGCAAAA ACTAATAGAT 
GTGAACCACT ATGCCAAGGA AGAAGTAGCA GCTCGGATGA ATGAGGTCAT CAGTTTGTGG 
AAGAAACTTC TAGAGGCCAC AGAACTGAAA GGAGTCAAGC TCCGAGAAGC CAACCAGCAG 
CAACAATTTA ATCGAAATGT TGAGGACATT GAGTTGTGGC TGTATGAAGT TGAAGGTCAC 
TTGGCTTCAG ATGATTATGG TAAAGACCTC ACTAATGTCC AGAACCTCCA GAAGAAGCAT 
GCTCTGCTAG AGGCAGATGT TGCTGCTCAC CAGGATCGAA TTGACGGCAT CACAATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTC GATGCCGAAA ACATTAAAAA GAAGCAAGAG 
GCCCTTGTAG CTCGCTATGA GGCTCTCAAG GAACCCATGG TGGCCCGGAA GCAGAAGCTG 
GCAGATTCTC TTCGTCTGCA GCAGCTCTTC CGAGATGTGG AGGATGAGGA AACCTGGATT 
CGAGAAAAGG AGCCTATTGC TGCGTCCACT AACAGAGGCA AAGATCTTAT TGGAGTCCAG 
AATCTGCTAA AGAAGCACCA AGCTTTACAG GCAGAAATTG CTGGCCATGA ACCTCGCATC 
AAAGCAGTGA CACAAAAGGG CAATGCCATG GTGGAGGAAG GCCATTTTGC TGCTGAGGAT 
GTGAAGGCCA AACTGAGTGA GCTCAACCAG AAGTGGGAGG CACTGAAAGC CAAAGCCTCC 
CAGCGGAGGC AGGATCTGGA GGACTCACTA CAGGCCCAGC AGTACTTTGC CGACGCCAAT 
GAAGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATTGTGG GCAGTACCGA CTATGGGAAG 
GATGAAGACT CTGCTGAGGC TCTGCTCAAG AAGCATGAAG CTTTGATGTC CGATCTCAGT 
GCCTACGGCA GCAGCATTCA AGCTTTGCGA GAGCAGGCTC AGTCATGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGCAAGGAG CTGGTCTTGG CTCTCTATGA CTATCAAGAG 
AAGAGCCCTC GTGAGGTCAC CATGAAGAAA GGGGATATCC TCACCTTGCT CAACAGCACA 
AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGTCAGG GTTTTGTGCC AGCTGCGTAT 
GTGAAGAAGC TGGACCCCGC CCAGTCAGCC TCAAGGGAGA ACCTCCTGGA AGAACAGGGC 
AGCATTGCTC TGCGGCAGGG GCAGATCGAC AACCAGACAC GCATAACTAA GGAGGCCGGC 
AGTGTATCTC TGCGTATGAA ACAGGTGGAA GAACTGTATC AGTCTCTGCT GGAGCTGGGT 
GAGAAGAGAA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TCATGTTGTT CCGGGAAGCG 
AACGAGCTAC AGCAGTGGAT CAACGAGAAG GAAGCTGCTC TAACGAGTGA AGAGGTTGGC 
GCTGACTTGG AGCAGGTCGA GGTGCTGCAG AAGAAGTTCG ATGACTTCCA GAAGGATCTG 
AAAGCCAATG AGTCCCGGCT GAAGGACATT AACAAAGTGG CCGAGGACCT GGAGTCTGAA 
GGTCTCATGG CGGAAGAAGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG TATGATGCCC 
AGGGATGAAG CAGATTCCAA GACCGCCTCC CCATGGAAGT CTGCTCGACT GATGGTCCAC 
ACAGTGGCCA CCTTCAACTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTGCAACAG 
CTGGCTGAGG AACGTAGCCA GCTCCTGGGC AGTGCACACG AAGTACAGAG GTTCCACAGG 
GATGCGGATG AAACCAAAGA ATGGATTGAG GAGAAGAACC AGGCTCTGAA CACAGACAAC 
TATGGCCATG ATCTAGCTAG CGTCCAGGCC CTGCAGCGCA AACACGAAGG CTTCGAGAGG 
GACCTTGCAG CTCTTGGTGA CAAGGTGAAT TCCCTTGGGG AAACAGCCCA GAGGCTGATC 
CAGTCCCACC CTGAATCTGC AGAGGACTTA AAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGACCAGCC TAGGGAAGCG TGCAGACCAG CGCAAGGCCA AACTGGGTGA CTCCCATGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGAGGG 
TTGGTATCTT CAGATGAACT GGCCAAGGAT GTCACTGGAG CTGAGGCTTT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATTGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TATGCCAGCC CAGAGATCAA GGAGAAACTT 
GATATTCTTG ACCAGGAGCG CACAGACCTG GAGAAGGCCT GGGTTCAGCG CAGAATGATG 
CTGGACCACT GCCTGGAGTT GCAGCTGTTC CATCGAGACT GTGAGCAAGC AGAGAACTGG 
ATGGCTGCCC GGGAAGCCTT CCTAAACACA GAAGACAAAG GAGACTCGCT GGACAGTGTG 
GAGGCTCTGA TCAAAAAACA TGAAGACTTC GACAAAGCTA TCAATGTCCA GGAGGAGAAG 
ATAGCTGCCC TGCAGGCCTT TGCCGACCAG CTCATTGCTG TGGACCACTA TGCCAAGGGA 
GACATTGCAA ACCGACGCAA TGAGGTCCTG GACAGGTGGC GCCGCCTAAA AGCCCAGATG 
ATTGAGAAAA GGTCAAAGCT CGGAGAATCT CAAACACTTC AGCAGTTCAG CCGGGATGTA 
GATGAGATTG AAGCCTGGAT CAGTGAGAAG TTACAAACAG CCAGCGATGA GTCATACAAG 
GACCCCACCA ACATCCAGAG CAAGCACCAG AAGCACCAAG CCTTTGAGGC AGAACTGCAC 
GCCAATGCTG ACCGAATCCG TGGAGTTATT GACATGGGCA ACTCCCTCAT TGAGCGTGGG 
GCCTGTGCTG GCAGTGAGGA TGCTGTCAAG GCCCGCCTGG CTGCCCTTGC AGACCAGTGG 
CAGTTCCTGG TGCAGAAGTC AGCTGAGAAG AGCCAGAAGC TGAAAGAGGC CAATAAGCAG 
CAGAACTTCA ACACCGGGAT CAAAGACTTT GACTTCTGGC TTTCTGAGGT GGAGGCTCTC 
CTGGCATCTG AAGACTACGG CAAAGACCTG GCTTCCGTGA ACAACCTGCT CAAAAAGCAT 
CAGCTGCTGG AGGCAGACAT ATCGGCCCAC GAGGATCGTC TGAAGGACCT GAACAGCCAG 
GCTGACAGCC TGATGACTAG CAGTGCCTTC GACACCTCCC AAGTGAAAGA GAAGCGGGAC 
ACCATCAATG GACGCTTTCA GAAGATCAAG AGCATGGCAA CCTCCCGAAG AGCAAAACTG 
AGCGAGTCCC ATCGCCTGCA CCAGTTTTTC CGAGACATGG ATGACGAGGA GTCCTGGATC 
AAGGAGAAGA AGTTGTTAGT GAGCTCTGAG GACTATGGCA GAGACCTCAC TGGTGTTCAA 
AATCTGAGGA AGAAACACAA GCGGCTAGAA GCCGAACTGG CCGCACACGA ACCAGCCATT 
CAGGGTGTCC TGGACACGGG GAAGAAGCTG TCTGATGACA ACACCATCGG GCAGGAGGAG 
ATCCAGCAGC GTCTCGCACA GTTTGTGGAG CACTGGAAGG AACTGAAACA GCTAGCAGCT 
GCACGGGGCC AGCGGCTGGA GGAGTCCTTG GAGTATCAGC AGTTTGTGGC CAACGTGGAG 
GAGGAGGAGG CTTGGATCAA TGAGAAGATG ACCCTGGTGG CCAGCGAAGA CTACGGGGAC 
ACTCTTGCTG CCATCCAGGG CTTACTGAAG AAACATGAAG CTTTTGAGAC AGACTTCACT 
GTCCACAAGG ATCGAGTGAA TGATGTCTGT ACTAATGGAC AAGACCTCAT TAAGAAGAAC 
AATCACCATG AGGAGAACAT CTCTTCAAAG ATGAAGGGTC TGAATGGTAA AGTGTCTGAC 
CTGGAGAAAG CAGCAGCTCA GAGGAAAGCG AAGCTGGATG AGAACTCGGC CTTCCTTCAG 
TTCAATTGGA AGGCTGACGT GGTGGAGTCC TGGATTGGTG AAAAGGAGAA CAGCTTGAAA 
ACAGATGATT ATGGCCGAGA TCTGTCTTCT GTCCAAACTC TGCTCACCAA GCAGGAGACA 
TTTGATGCTG GCCTGCAGGC CTTCCAGCAG GAGGGCATTG CCAATATCAC TGCCCTCAAA 
GACCAGCTGC TAGCTGCCAA GCACATTCAG TCGAAGGCCA TCGAGGCCCG ACATGCCTCC 
CTCATGAAGA GGTGGACCCA GCTGTTGGCC AATTCAGCTA CCCGCAAGAA GAAGTTGCTA 
GAGGCCCAGA GTCATTTCCG AAAGGTAGAA GACCTCTTCC TGACCTTTGC CAAAAAGGCA 
TCGGCTTTCA ACAGCTGGTT TGAGAATGCA GAAGAGGACC TCACAGACCC AGTGCGCTGC 
AACTCTCTGG AAGAAATCAA AGCCCTCCGA GAGGCTCATG ATGCCTTCCG CTCATCGCTC 
AGCTCTGCGC AGGCCGACTT CAACCAGCTA GCCGAGCTGG ACCGTCAGAT CAAGAGTTTC 
CGAGTGGCCT CCAATCCCTA CACCTGGTTC ACCATGGAGG CCCTGGAAGA GACGTGGAGG 
AACCTACAGA AGATCATTAA GGAGCGAGAA CTGGAGCTGC AGAAGGAACA GCGGCGGCAG 
GAGGAGAATG ACAAGCTACG CCAAGAGTTT GCCCAGCATG CCAACGCGTT CCACCAGTGG 
ATCCAGGAAA CAAGAACGTA TCTCCTCGAC GGCATAGCAT ATCGTCGGGT CATTCGTGTC 
TATCAGTATG AAGTTGGGGA TGATCTGTCT GGAAGGTCCT GCATGGTCGA AGAGTCGGGA 
ACTCTGGAAT CTCAGCTTGA AGCTACCAAA CGCAAGCACC AGGAGATTCG GGCCATGAGA 
AGTCAGCTGA AGAAGATTGA GGACCTGGGG GCTGCCATGG AGGAAGCCCT CATCCTGGAC 
AACAAGTACA CTGAGCACAG CACTGTGGGC CTGGCCCAGC AGTGGGACCA GTTAGACCAG 
CTGGGCATGC GCATGCAGCA CAACCTGGAG CAGCAGATCC AGGCCAGGAA CACAACAGGA 
GTCACTGAGG AGGCCCTCAA GGAGTTCAGC ATGATGTTCA AACACTTCGA CAAGGACAAG 
TCTGGCCGGC TGAATCATCA AGAGTTCAAA TCCTGCCTTC GTTCTCTGGG TTACGACCTG 
CCAATGGTTG AGGAAGGAGA GCCTGATCCT GAGTTTGAGG CCATACTGGA CACTGTTGAT 
CCCAACAGGG ACGGCCACGT CTCCCTGCAA GAGTACATGG CTTTCATGAT CAGCCGTGAA 
ACCGAGAATG TCAAGTCCAG TGAAGAGATC GAGAGTGCTT TCCGGGCCCT CAGCTCCGAG 
GGCAAGCCTT ATGTGACCAA GGAGGAGCTC TACCAGAACC TGACCCGGGA ACAAGCTGAC 
TACTGTGTCT CCCACATGAA GCCCTATGTG GATGGCAAGG GCCGCGAACT TCCAACTGCC 
TTCGACTACG TGGAGTTCAC CCGCTCTCTC TTTGTGAATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sptan1 ( XM_008761675.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008759897.1 Rattus norvegicus spectrin, alpha, non-erythrocytic 1 (Sptan1), transcript variant X3, mRNA.

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CloneID	ORa43562
Clone ID Related Accession (Same CDS sequence)	XM_008761675.2
Accession Version	XM_008761675.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7437bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008761675.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCAA GTGGGGTCAA AGTGCTGGAA ACAGCCGAGG ACATCCAGGA GAGACGACAG 
CAGGTTCTGG ATCGGTACCA CCGCTTCAAG GAGCTCTCTA CCTTGCGGCG GCAGAAACTG 
GAGGATTCCT ATCGCTTCCA GTTTTTTCAG AGAGATGCTG AGGAGCTGGA GAAGTGGATT 
CAGGAGAAGC TTCAAGTTGC CTCTGATGAG AACTACAAAG ACCCCACCAA CTTGCAGGGA 
AAGCTCCAGA AACACCAAGC CTTTGAAGCT GAAGTACAGG CCAACTCAGG AGCCATTGTG 
AAGCTGGATG AGACAGGAAA CTTGATGATT TCTGAAGGGC ACTTTGCATC TGAGACCATC 
CGGACACGTT TAATGGAGCT GCACCGCCAG TGGGAGTTGC TTTTGGAGAA GATGCGGGAG 
AAAGGAATCA AACTGCTGCA GGCACAGAAG CTGGTGCAGT ATTTGCGGGA GTGTGAGGAT 
GTAATGGACT GGATCAATGA CAAGGAAGCA ATTGTGACGT CTGAGGAGCT GGGCCAGGAT 
TTGGAGCATG TAGAGGTACT ACAGAAGAAG TTTGAAGAGT TTCAAACTGA TCTGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTAAACCAG TTTGCTGCCA AACTTATCCA GGAGCAGCAC 
CCGGAAGAGG AGCTGATCAA GACCAAGCAG GAGGAGGTGA ATGCAGCTTG GCAGCGACTG 
AAAGGCCTGG CTCTTCAAAG GCAGGGGAAG CTCTTTGGTG CTGCCGAGGT TCAGCGCTTT 
AACAGGGATG TAGATGAGAC CATTGGTTGG ATTAAGGAGA AAGAGCAGTT AATGGCCTCT 
GATGACTTTG GCAGAGACTT AGCAAGTGTT CAAGCTCTGC TTCGGAAGCA TGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAGGACAAG GTGAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCAACAGT CACACCCTCT GAGTGCCAAC CAGATCCAGG TGAAGCGAGA GGAACTAATT 
ACCAACTGGG AGCAGATCCG AACTCTGGCC GCAGAGAGAC ATGCACGGCT TGATGACTCA 
TACAGGCTTC AGCGCTTTCT TGCTGACTTC CGTGACCTCA CGAGCTGGGT GACTGAAATG 
AAAGCCCTCA TCAATGCAGA TGAACTGGCC AATGACGTGG CTGGTGCTGA GGCCCTGCTG 
GACAGGCATC AAGAGCACAA GGGTGAAATC GATGCTCATG AAGATAGCTT TAAGTCTGCA 
GATGAGTCTG GGCAGGCCCT ACTCGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGAGCA TCCTCTCTGA GGAGAGAGCT GCCCTGCTGG AGCTGTGGGA GCTTCGGAGG 
CAACAGTATG AGCAGTGCAT GGACTTGCAG CTCTTCTACC GAGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAACAGGA GGCATTCCTG CTAAATGAAG ATTTGGGTGA CTCCTTAGAC 
AGTGTGGAAG CTCTTTTGAA GAAGCATGAG GACTTTGAGA AATCTCTCAG TGCCCAGGAA 
GAAAAAATCA CAGCACTTGA TGAGTTTGCA ACCAAGCTTA TTCAGAACAA CCACTACGCA 
ATGGAAGATG TAGCCACTCG ACGAGATGCT CTCCTGAGCC GCCGCAATGC CCTCCATGAG 
CGAGCCATGC ATCGCCGGGC ACAGCTGGCC GATTCCTTCC ACCTGCAGCA GTTCTTCCGC 
GATTCCGATG AGCTCAAAAG TTGGGTCAAT GAGAAGATGA AAACGGCCAC TGATGAAGCT 
TACAAAGATC CGTCCAACCT GCAAGGGAAA GTCCAAAAGC ACCAGGCTTT TGAGGCTGAG 
CTCTCAGCCA ACCAGAGCCG TATTGATGCC CTAGAGAAAG CTGGGCAAAA ACTAATAGAT 
GTGAACCACT ATGCCAAGGA AGAAGTAGCA GCTCGGATGA ATGAGGTCAT CAGTTTGTGG 
AAGAAACTTC TAGAGGCCAC AGAACTGAAA GGAGTCAAGC TCCGAGAAGC CAACCAGCAG 
CAACAATTTA ATCGAAATGT TGAGGACATT GAGTTGTGGC TGTATGAAGT TGAAGGTCAC 
TTGGCTTCAG ATGATTATGG TAAAGACCTC ACTAATGTCC AGAACCTCCA GAAGAAGCAT 
GCTCTGCTAG AGGCAGATGT TGCTGCTCAC CAGGATCGAA TTGACGGCAT CACAATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTC GATGCCGAAA ACATTAAAAA GAAGCAAGAG 
GCCCTTGTAG CTCGCTATGA GGCTCTCAAG GAACCCATGG TGGCCCGGAA GCAGAAGCTG 
GCAGATTCTC TTCGTCTGCA GCAGCTCTTC CGAGATGTGG AGGATGAGGA AACCTGGATT 
CGAGAAAAGG AGCCTATTGC TGCGTCCACT AACAGAGGCA AAGATCTTAT TGGAGTCCAG 
AATCTGCTAA AGAAGCACCA AGCTTTACAG GCAGAAATTG CTGGCCATGA ACCTCGCATC 
AAAGCAGTGA CACAAAAGGG CAATGCCATG GTGGAGGAAG GCCATTTTGC TGCTGAGGAT 
GTGAAGGCCA AACTGAGTGA GCTCAACCAG AAGTGGGAGG CACTGAAAGC CAAAGCCTCC 
CAGCGGAGGC AGGATCTGGA GGACTCACTA CAGGCCCAGC AGTACTTTGC CGACGCCAAT 
GAAGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATTGTGG GCAGTACCGA CTATGGGAAG 
GATGAAGACT CTGCTGAGGC TCTGCTCAAG AAGCATGAAG CTTTGATGTC CGATCTCAGT 
GCCTACGGCA GCAGCATTCA AGCTTTGCGA GAGCAGGCTC AGTCATGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGCAAGGAG CTGGTCTTGG CTCTCTATGA CTATCAAGAG 
AAGAGCCCTC GTGAGGTCAC CATGAAGAAA GGGGATATCC TCACCTTGCT CAACAGCACA 
AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGTCAGG GTTTTGTGCC AGCTGCGTAT 
GTGAAGAAGC TGGACCCCGC CCAGTCAGCC TCAAGGGAGA ACCTCCTGGA AGAACAGGGC 
AGCATTGCTC TGCGGCAGGG GCAGATCGAC AACCAGTATC AGTCTCTGCT GGAGCTGGGT 
GAGAAGAGAA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TCATGTTGTT CCGGGAAGCG 
AACGAGCTAC AGCAGTGGAT CAACGAGAAG GAAGCTGCTC TAACGAGTGA AGAGGTTGGC 
GCTGACTTGG AGCAGGTCGA GGTGCTGCAG AAGAAGTTCG ATGACTTCCA GAAGGATCTG 
AAAGCCAATG AGTCCCGGCT GAAGGACATT AACAAAGTGG CCGAGGACCT GGAGTCTGAA 
GGTCTCATGG CGGAAGAAGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG TATGATGCCC 
AGGGATGAAG CAGATTCCAA GACCGCCTCC CCATGGAAGT CTGCTCGACT GATGGTCCAC 
ACAGTGGCCA CCTTCAACTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTGCAACAG 
CTGGCTGAGG AACGTAGCCA GCTCCTGGGC AGTGCACACG AAGTACAGAG GTTCCACAGG 
GATGCGGATG AAACCAAAGA ATGGATTGAG GAGAAGAACC AGGCTCTGAA CACAGACAAC 
TATGGCCATG ATCTAGCTAG CGTCCAGGCC CTGCAGCGCA AACACGAAGG CTTCGAGAGG 
GACCTTGCAG CTCTTGGTGA CAAGGTGAAT TCCCTTGGGG AAACAGCCCA GAGGCTGATC 
CAGTCCCACC CTGAATCTGC AGAGGACTTA AAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGACCAGCC TAGGGAAGCG TGCAGACCAG CGCAAGGCCA AACTGGGTGA CTCCCATGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGAGGG 
TTGGTATCTT CAGATGAACT GGCCAAGGAT GTCACTGGAG CTGAGGCTTT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATTGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TATGCCAGCC CAGAGATCAA GGAGAAACTT 
GATATTCTTG ACCAGGAGCG CACAGACCTG GAGAAGGCCT GGGTTCAGCG CAGAATGATG 
CTGGACCACT GCCTGGAGTT GCAGCTGTTC CATCGAGACT GTGAGCAAGC AGAGAACTGG 
ATGGCTGCCC GGGAAGCCTT CCTAAACACA GAAGACAAAG GAGACTCGCT GGACAGTGTG 
GAGGCTCTGA TCAAAAAACA TGAAGACTTC GACAAAGCTA TCAATGTCCA GGAGGAGAAG 
ATAGCTGCCC TGCAGGCCTT TGCCGACCAG CTCATTGCTG TGGACCACTA TGCCAAGGGA 
GACATTGCAA ACCGACGCAA TGAGGTCCTG GACAGGTGGC GCCGCCTAAA AGCCCAGATG 
ATTGAGAAAA GGTCAAAGCT CGGAGAATCT CAAACACTTC AGCAGTTCAG CCGGGATGTA 
GATGAGATTG AAGCCTGGAT CAGTGAGAAG TTACAAACAG CCAGCGATGA GTCATACAAG 
GACCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ACCAGAAGCA CCAAGCCTTT 
GAGGCAGAAC TGCACGCCAA TGCTGACCGA ATCCGTGGAG TTATTGACAT GGGCAACTCC 
CTCATTGAGC GTGGGGCCTG TGCTGGCAGT GAGGATGCTG TCAAGGCCCG CCTGGCTGCC 
CTTGCAGACC AGTGGCAGTT CCTGGTGCAG AAGTCAGCTG AGAAGAGCCA GAAGCTGAAA 
GAGGCCAATA AGCAGCAGAA CTTCAACACC GGGATCAAAG ACTTTGACTT CTGGCTTTCT 
GAGGTGGAGG CTCTCCTGGC ATCTGAAGAC TACGGCAAAG ACCTGGCTTC CGTGAACAAC 
CTGCTCAAAA AGCATCAGCT GCTGGAGGCA GACATATCGG CCCACGAGGA TCGTCTGAAG 
GACCTGAACA GCCAGGCTGA CAGCCTGATG ACTAGCAGTG CCTTCGACAC CTCCCAAGTG 
AAAGAGAAGC GGGACACCAT CAATGGACGC TTTCAGAAGA TCAAGAGCAT GGCAACCTCC 
CGAAGAGCAA AACTGAGCGA GTCCCATCGC CTGCACCAGT TTTTCCGAGA CATGGATGAC 
GAGGAGTCCT GGATCAAGGA GAAGAAGTTG TTAGTGAGCT CTGAGGACTA TGGCAGAGAC 
CTCACTGGTG TTCAAAATCT GAGGAAGAAA CACAAGCGGC TAGAAGCCGA ACTGGCCGCA 
CACGAACCAG CCATTCAGGG TGTCCTGGAC ACGGGGAAGA AGCTGTCTGA TGACAACACC 
ATCGGGCAGG AGGAGATCCA GCAGCGTCTC GCACAGTTTG TGGAGCACTG GAAGGAACTG 
AAACAGCTAG CAGCTGCACG GGGCCAGCGG CTGGAGGAGT CCTTGGAGTA TCAGCAGTTT 
GTGGCCAACG TGGAGGAGGA GGAGGCTTGG ATCAATGAGA AGATGACCCT GGTGGCCAGC 
GAAGACTACG GGGACACTCT TGCTGCCATC CAGGGCTTAC TGAAGAAACA TGAAGCTTTT 
GAGACAGACT TCACTGTCCA CAAGGATCGA GTGAATGATG TCTGTACTAA TGGACAAGAC 
CTCATTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CAAAGATGAA GGGTCTGAAT 
GGTAAAGTGT CTGACCTGGA GAAAGCAGCA GCTCAGAGGA AAGCGAAGCT GGATGAGAAC 
TCGGCCTTCC TTCAGTTCAA TTGGAAGGCT GACGTGGTGG AGTCCTGGAT TGGTGAAAAG 
GAGAACAGCT TGAAAACAGA TGATTATGGC CGAGATCTGT CTTCTGTCCA AACTCTGCTC 
ACCAAGCAGG AGACATTTGA TGCTGGCCTG CAGGCCTTCC AGCAGGAGGG CATTGCCAAT 
ATCACTGCCC TCAAAGACCA GCTGCTAGCT GCCAAGCACA TTCAGTCGAA GGCCATCGAG 
GCCCGACATG CCTCCCTCAT GAAGAGGTGG ACCCAGCTGT TGGCCAATTC AGCTACCCGC 
AAGAAGAAGT TGCTAGAGGC CCAGAGTCAT TTCCGAAAGG TAGAAGACCT CTTCCTGACC 
TTTGCCAAAA AGGCATCGGC TTTCAACAGC TGGTTTGAGA ATGCAGAAGA GGACCTCACA 
GACCCAGTGC GCTGCAACTC TCTGGAAGAA ATCAAAGCCC TCCGAGAGGC TCATGATGCC 
TTCCGCTCAT CGCTCAGCTC TGCGCAGGCC GACTTCAACC AGCTAGCCGA GCTGGACCGT 
CAGATCAAGA GTTTCCGAGT GGCCTCCAAT CCCTACACCT GGTTCACCAT GGAGGCCCTG 
GAAGAGACGT GGAGGAACCT ACAGAAGATC ATTAAGGAGC GAGAACTGGA GCTGCAGAAG 
GAACAGCGGC GGCAGGAGGA GAATGACAAG CTACGCCAAG AGTTTGCCCA GCATGCCAAC 
GCGTTCCACC AGTGGATCCA GGAAACAAGA ACGTATCTCC TCGACGGCAT AGCATATCGT 
CGGGTCATTC GTGTCTATCA GTATGAAGTT GGGGATGATC TGTCTGGAAG GTCCTGCATG 
GTCGAAGAGT CGGGAACTCT GGAATCTCAG CTTGAAGCTA CCAAACGCAA GCACCAGGAG 
ATTCGGGCCA TGAGAAGTCA GCTGAAGAAG ATTGAGGACC TGGGGGCTGC CATGGAGGAA 
GCCCTCATCC TGGACAACAA GTACACTGAG CACAGCACTG TGGGCCTGGC CCAGCAGTGG 
GACCAGTTAG ACCAGCTGGG CATGCGCATG CAGCACAACC TGGAGCAGCA GATCCAGGCC 
AGGAACACAA CAGGAGTCAC TGAGGAGGCC CTCAAGGAGT TCAGCATGAT GTTCAAACAC 
TTCGACAAGG ACAAGTCTGG CCGGCTGAAT CATCAAGAGT TCAAATCCTG CCTTCGTTCT 
CTGGGTTACG ACCTGCCAAT GGTTGAGGAA GGAGAGCCTG ATCCTGAGTT TGAGGCCATA 
CTGGACACTG TTGATCCCAA CAGGGACGGC CACGTCTCCC TGCAAGAGTA CATGGCTTTC 
ATGATCAGCC GTGAAACCGA GAATGTCAAG TCCAGTGAAG AGATCGAGAG TGCTTTCCGG 
GCCCTCAGCT CCGAGGGCAA GCCTTATGTG ACCAAGGAGG AGCTCTACCA GAACCTGACC 
CGGGAACAAG CTGACTACTG TGTCTCCCAC ATGAAGCCCT ATGTGGATGG CAAGGGCCGC 
GAACTTCCAA CTGCCTTCGA CTACGTGGAG TTCACCCGCT CTCTCTTTGT GAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008759897.1
CDS	112..7548
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQVASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDLAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQEEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETIGWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSANQIQVKREELITNWEQIRTLAAERHARLDDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLSILSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMHRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKEEVAARMNEVISLWKKLLEATELKGVKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLSELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQGQIDNQYQSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGMMPRDEADSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAQRLIQSHPESAEDLKEKCTELNQAWTSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKEKLDILDQERTDLEKAWVQRRMMLDHCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAVDHYAKGDIANRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKEKRDTINGRFQKIKSMATSRRAKLSESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGQEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHIQSKAIEARHASLMKRWTQLLANSATRKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGIAYRRVIRVYQYEVGDDLSGRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_008761675.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus spectrin, alpha, non-erythrocytic 1 (Sptan1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008761675.2

ORF Insert Sequence:

ATGGATCCAA GTGGGGTCAA AGTGCTGGAA ACAGCCGAGG ACATCCAGGA GAGACGACAG 
CAGGTTCTGG ATCGGTACCA CCGCTTCAAG GAGCTCTCTA CCTTGCGGCG GCAGAAACTG 
GAGGATTCCT ATCGCTTCCA GTTTTTTCAG AGAGATGCTG AGGAGCTGGA GAAGTGGATT 
CAGGAGAAGC TTCAAGTTGC CTCTGATGAG AACTACAAAG ACCCCACCAA CTTGCAGGGA 
AAGCTCCAGA AACACCAAGC CTTTGAAGCT GAAGTACAGG CCAACTCAGG AGCCATTGTG 
AAGCTGGATG AGACAGGAAA CTTGATGATT TCTGAAGGGC ACTTTGCATC TGAGACCATC 
CGGACACGTT TAATGGAGCT GCACCGCCAG TGGGAGTTGC TTTTGGAGAA GATGCGGGAG 
AAAGGAATCA AACTGCTGCA GGCACAGAAG CTGGTGCAGT ATTTGCGGGA GTGTGAGGAT 
GTAATGGACT GGATCAATGA CAAGGAAGCA ATTGTGACGT CTGAGGAGCT GGGCCAGGAT 
TTGGAGCATG TAGAGGTACT ACAGAAGAAG TTTGAAGAGT TTCAAACTGA TCTGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTAAACCAG TTTGCTGCCA AACTTATCCA GGAGCAGCAC 
CCGGAAGAGG AGCTGATCAA GACCAAGCAG GAGGAGGTGA ATGCAGCTTG GCAGCGACTG 
AAAGGCCTGG CTCTTCAAAG GCAGGGGAAG CTCTTTGGTG CTGCCGAGGT TCAGCGCTTT 
AACAGGGATG TAGATGAGAC CATTGGTTGG ATTAAGGAGA AAGAGCAGTT AATGGCCTCT 
GATGACTTTG GCAGAGACTT AGCAAGTGTT CAAGCTCTGC TTCGGAAGCA TGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAGGACAAG GTGAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCAACAGT CACACCCTCT GAGTGCCAAC CAGATCCAGG TGAAGCGAGA GGAACTAATT 
ACCAACTGGG AGCAGATCCG AACTCTGGCC GCAGAGAGAC ATGCACGGCT TGATGACTCA 
TACAGGCTTC AGCGCTTTCT TGCTGACTTC CGTGACCTCA CGAGCTGGGT GACTGAAATG 
AAAGCCCTCA TCAATGCAGA TGAACTGGCC AATGACGTGG CTGGTGCTGA GGCCCTGCTG 
GACAGGCATC AAGAGCACAA GGGTGAAATC GATGCTCATG AAGATAGCTT TAAGTCTGCA 
GATGAGTCTG GGCAGGCCCT ACTCGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGAGCA TCCTCTCTGA GGAGAGAGCT GCCCTGCTGG AGCTGTGGGA GCTTCGGAGG 
CAACAGTATG AGCAGTGCAT GGACTTGCAG CTCTTCTACC GAGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAACAGGA GGCATTCCTG CTAAATGAAG ATTTGGGTGA CTCCTTAGAC 
AGTGTGGAAG CTCTTTTGAA GAAGCATGAG GACTTTGAGA AATCTCTCAG TGCCCAGGAA 
GAAAAAATCA CAGCACTTGA TGAGTTTGCA ACCAAGCTTA TTCAGAACAA CCACTACGCA 
ATGGAAGATG TAGCCACTCG ACGAGATGCT CTCCTGAGCC GCCGCAATGC CCTCCATGAG 
CGAGCCATGC ATCGCCGGGC ACAGCTGGCC GATTCCTTCC ACCTGCAGCA GTTCTTCCGC 
GATTCCGATG AGCTCAAAAG TTGGGTCAAT GAGAAGATGA AAACGGCCAC TGATGAAGCT 
TACAAAGATC CGTCCAACCT GCAAGGGAAA GTCCAAAAGC ACCAGGCTTT TGAGGCTGAG 
CTCTCAGCCA ACCAGAGCCG TATTGATGCC CTAGAGAAAG CTGGGCAAAA ACTAATAGAT 
GTGAACCACT ATGCCAAGGA AGAAGTAGCA GCTCGGATGA ATGAGGTCAT CAGTTTGTGG 
AAGAAACTTC TAGAGGCCAC AGAACTGAAA GGAGTCAAGC TCCGAGAAGC CAACCAGCAG 
CAACAATTTA ATCGAAATGT TGAGGACATT GAGTTGTGGC TGTATGAAGT TGAAGGTCAC 
TTGGCTTCAG ATGATTATGG TAAAGACCTC ACTAATGTCC AGAACCTCCA GAAGAAGCAT 
GCTCTGCTAG AGGCAGATGT TGCTGCTCAC CAGGATCGAA TTGACGGCAT CACAATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTC GATGCCGAAA ACATTAAAAA GAAGCAAGAG 
GCCCTTGTAG CTCGCTATGA GGCTCTCAAG GAACCCATGG TGGCCCGGAA GCAGAAGCTG 
GCAGATTCTC TTCGTCTGCA GCAGCTCTTC CGAGATGTGG AGGATGAGGA AACCTGGATT 
CGAGAAAAGG AGCCTATTGC TGCGTCCACT AACAGAGGCA AAGATCTTAT TGGAGTCCAG 
AATCTGCTAA AGAAGCACCA AGCTTTACAG GCAGAAATTG CTGGCCATGA ACCTCGCATC 
AAAGCAGTGA CACAAAAGGG CAATGCCATG GTGGAGGAAG GCCATTTTGC TGCTGAGGAT 
GTGAAGGCCA AACTGAGTGA GCTCAACCAG AAGTGGGAGG CACTGAAAGC CAAAGCCTCC 
CAGCGGAGGC AGGATCTGGA GGACTCACTA CAGGCCCAGC AGTACTTTGC CGACGCCAAT 
GAAGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATTGTGG GCAGTACCGA CTATGGGAAG 
GATGAAGACT CTGCTGAGGC TCTGCTCAAG AAGCATGAAG CTTTGATGTC CGATCTCAGT 
GCCTACGGCA GCAGCATTCA AGCTTTGCGA GAGCAGGCTC AGTCATGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGCAAGGAG CTGGTCTTGG CTCTCTATGA CTATCAAGAG 
AAGAGCCCTC GTGAGGTCAC CATGAAGAAA GGGGATATCC TCACCTTGCT CAACAGCACA 
AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGTCAGG GTTTTGTGCC AGCTGCGTAT 
GTGAAGAAGC TGGACCCCGC CCAGTCAGCC TCAAGGGAGA ACCTCCTGGA AGAACAGGGC 
AGCATTGCTC TGCGGCAGGG GCAGATCGAC AACCAGTATC AGTCTCTGCT GGAGCTGGGT 
GAGAAGAGAA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TCATGTTGTT CCGGGAAGCG 
AACGAGCTAC AGCAGTGGAT CAACGAGAAG GAAGCTGCTC TAACGAGTGA AGAGGTTGGC 
GCTGACTTGG AGCAGGTCGA GGTGCTGCAG AAGAAGTTCG ATGACTTCCA GAAGGATCTG 
AAAGCCAATG AGTCCCGGCT GAAGGACATT AACAAAGTGG CCGAGGACCT GGAGTCTGAA 
GGTCTCATGG CGGAAGAAGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG TATGATGCCC 
AGGGATGAAG CAGATTCCAA GACCGCCTCC CCATGGAAGT CTGCTCGACT GATGGTCCAC 
ACAGTGGCCA CCTTCAACTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTGCAACAG 
CTGGCTGAGG AACGTAGCCA GCTCCTGGGC AGTGCACACG AAGTACAGAG GTTCCACAGG 
GATGCGGATG AAACCAAAGA ATGGATTGAG GAGAAGAACC AGGCTCTGAA CACAGACAAC 
TATGGCCATG ATCTAGCTAG CGTCCAGGCC CTGCAGCGCA AACACGAAGG CTTCGAGAGG 
GACCTTGCAG CTCTTGGTGA CAAGGTGAAT TCCCTTGGGG AAACAGCCCA GAGGCTGATC 
CAGTCCCACC CTGAATCTGC AGAGGACTTA AAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGACCAGCC TAGGGAAGCG TGCAGACCAG CGCAAGGCCA AACTGGGTGA CTCCCATGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGAGGG 
TTGGTATCTT CAGATGAACT GGCCAAGGAT GTCACTGGAG CTGAGGCTTT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATTGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TATGCCAGCC CAGAGATCAA GGAGAAACTT 
GATATTCTTG ACCAGGAGCG CACAGACCTG GAGAAGGCCT GGGTTCAGCG CAGAATGATG 
CTGGACCACT GCCTGGAGTT GCAGCTGTTC CATCGAGACT GTGAGCAAGC AGAGAACTGG 
ATGGCTGCCC GGGAAGCCTT CCTAAACACA GAAGACAAAG GAGACTCGCT GGACAGTGTG 
GAGGCTCTGA TCAAAAAACA TGAAGACTTC GACAAAGCTA TCAATGTCCA GGAGGAGAAG 
ATAGCTGCCC TGCAGGCCTT TGCCGACCAG CTCATTGCTG TGGACCACTA TGCCAAGGGA 
GACATTGCAA ACCGACGCAA TGAGGTCCTG GACAGGTGGC GCCGCCTAAA AGCCCAGATG 
ATTGAGAAAA GGTCAAAGCT CGGAGAATCT CAAACACTTC AGCAGTTCAG CCGGGATGTA 
GATGAGATTG AAGCCTGGAT CAGTGAGAAG TTACAAACAG CCAGCGATGA GTCATACAAG 
GACCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ACCAGAAGCA CCAAGCCTTT 
GAGGCAGAAC TGCACGCCAA TGCTGACCGA ATCCGTGGAG TTATTGACAT GGGCAACTCC 
CTCATTGAGC GTGGGGCCTG TGCTGGCAGT GAGGATGCTG TCAAGGCCCG CCTGGCTGCC 
CTTGCAGACC AGTGGCAGTT CCTGGTGCAG AAGTCAGCTG AGAAGAGCCA GAAGCTGAAA 
GAGGCCAATA AGCAGCAGAA CTTCAACACC GGGATCAAAG ACTTTGACTT CTGGCTTTCT 
GAGGTGGAGG CTCTCCTGGC ATCTGAAGAC TACGGCAAAG ACCTGGCTTC CGTGAACAAC 
CTGCTCAAAA AGCATCAGCT GCTGGAGGCA GACATATCGG CCCACGAGGA TCGTCTGAAG 
GACCTGAACA GCCAGGCTGA CAGCCTGATG ACTAGCAGTG CCTTCGACAC CTCCCAAGTG 
AAAGAGAAGC GGGACACCAT CAATGGACGC TTTCAGAAGA TCAAGAGCAT GGCAACCTCC 
CGAAGAGCAA AACTGAGCGA GTCCCATCGC CTGCACCAGT TTTTCCGAGA CATGGATGAC 
GAGGAGTCCT GGATCAAGGA GAAGAAGTTG TTAGTGAGCT CTGAGGACTA TGGCAGAGAC 
CTCACTGGTG TTCAAAATCT GAGGAAGAAA CACAAGCGGC TAGAAGCCGA ACTGGCCGCA 
CACGAACCAG CCATTCAGGG TGTCCTGGAC ACGGGGAAGA AGCTGTCTGA TGACAACACC 
ATCGGGCAGG AGGAGATCCA GCAGCGTCTC GCACAGTTTG TGGAGCACTG GAAGGAACTG 
AAACAGCTAG CAGCTGCACG GGGCCAGCGG CTGGAGGAGT CCTTGGAGTA TCAGCAGTTT 
GTGGCCAACG TGGAGGAGGA GGAGGCTTGG ATCAATGAGA AGATGACCCT GGTGGCCAGC 
GAAGACTACG GGGACACTCT TGCTGCCATC CAGGGCTTAC TGAAGAAACA TGAAGCTTTT 
GAGACAGACT TCACTGTCCA CAAGGATCGA GTGAATGATG TCTGTACTAA TGGACAAGAC 
CTCATTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CAAAGATGAA GGGTCTGAAT 
GGTAAAGTGT CTGACCTGGA GAAAGCAGCA GCTCAGAGGA AAGCGAAGCT GGATGAGAAC 
TCGGCCTTCC TTCAGTTCAA TTGGAAGGCT GACGTGGTGG AGTCCTGGAT TGGTGAAAAG 
GAGAACAGCT TGAAAACAGA TGATTATGGC CGAGATCTGT CTTCTGTCCA AACTCTGCTC 
ACCAAGCAGG AGACATTTGA TGCTGGCCTG CAGGCCTTCC AGCAGGAGGG CATTGCCAAT 
ATCACTGCCC TCAAAGACCA GCTGCTAGCT GCCAAGCACA TTCAGTCGAA GGCCATCGAG 
GCCCGACATG CCTCCCTCAT GAAGAGGTGG ACCCAGCTGT TGGCCAATTC AGCTACCCGC 
AAGAAGAAGT TGCTAGAGGC CCAGAGTCAT TTCCGAAAGG TAGAAGACCT CTTCCTGACC 
TTTGCCAAAA AGGCATCGGC TTTCAACAGC TGGTTTGAGA ATGCAGAAGA GGACCTCACA 
GACCCAGTGC GCTGCAACTC TCTGGAAGAA ATCAAAGCCC TCCGAGAGGC TCATGATGCC 
TTCCGCTCAT CGCTCAGCTC TGCGCAGGCC GACTTCAACC AGCTAGCCGA GCTGGACCGT 
CAGATCAAGA GTTTCCGAGT GGCCTCCAAT CCCTACACCT GGTTCACCAT GGAGGCCCTG 
GAAGAGACGT GGAGGAACCT ACAGAAGATC ATTAAGGAGC GAGAACTGGA GCTGCAGAAG 
GAACAGCGGC GGCAGGAGGA GAATGACAAG CTACGCCAAG AGTTTGCCCA GCATGCCAAC 
GCGTTCCACC AGTGGATCCA GGAAACAAGA ACGTATCTCC TCGACGGCAT AGCATATCGT 
CGGGTCATTC GTGTCTATCA GTATGAAGTT GGGGATGATC TGTCTGGAAG GTCCTGCATG 
GTCGAAGAGT CGGGAACTCT GGAATCTCAG CTTGAAGCTA CCAAACGCAA GCACCAGGAG 
ATTCGGGCCA TGAGAAGTCA GCTGAAGAAG ATTGAGGACC TGGGGGCTGC CATGGAGGAA 
GCCCTCATCC TGGACAACAA GTACACTGAG CACAGCACTG TGGGCCTGGC CCAGCAGTGG 
GACCAGTTAG ACCAGCTGGG CATGCGCATG CAGCACAACC TGGAGCAGCA GATCCAGGCC 
AGGAACACAA CAGGAGTCAC TGAGGAGGCC CTCAAGGAGT TCAGCATGAT GTTCAAACAC 
TTCGACAAGG ACAAGTCTGG CCGGCTGAAT CATCAAGAGT TCAAATCCTG CCTTCGTTCT 
CTGGGTTACG ACCTGCCAAT GGTTGAGGAA GGAGAGCCTG ATCCTGAGTT TGAGGCCATA 
CTGGACACTG TTGATCCCAA CAGGGACGGC CACGTCTCCC TGCAAGAGTA CATGGCTTTC 
ATGATCAGCC GTGAAACCGA GAATGTCAAG TCCAGTGAAG AGATCGAGAG TGCTTTCCGG 
GCCCTCAGCT CCGAGGGCAA GCCTTATGTG ACCAAGGAGG AGCTCTACCA GAACCTGACC 
CGGGAACAAG CTGACTACTG TGTCTCCCAC ATGAAGCCCT ATGTGGATGG CAAGGGCCGC 
GAACTTCCAA CTGCCTTCGA CTACGTGGAG TTCACCCGCT CTCTCTTTGT GAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sptan1 ( XM_008761676.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008759898.1 Rattus norvegicus spectrin, alpha, non-erythrocytic 1 (Sptan1), transcript variant X4, mRNA.

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CloneID	ORa43563
Clone ID Related Accession (Same CDS sequence)	XM_008761676.2
Accession Version	XM_008761676.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7434bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008761676.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCAA GTGGGGTCAA AGTGCTGGAA ACAGCCGAGG ACATCCAGGA GAGACGACAG 
CAGGTTCTGG ATCGGTACCA CCGCTTCAAG GAGCTCTCTA CCTTGCGGCG GCAGAAACTG 
GAGGATTCCT ATCGCTTCCA GTTTTTTCAG AGAGATGCTG AGGAGCTGGA GAAGTGGATT 
CAGGAGAAGC TTCAAGTTGC CTCTGATGAG AACTACAAAG ACCCCACCAA CTTGCAGGGA 
AAGCTCCAGA AACACCAAGC CTTTGAAGCT GAAGTACAGG CCAACTCAGG AGCCATTGTG 
AAGCTGGATG AGACAGGAAA CTTGATGATT TCTGAAGGGC ACTTTGCATC TGAGACCATC 
CGGACACGTT TAATGGAGCT GCACCGCCAG TGGGAGTTGC TTTTGGAGAA GATGCGGGAG 
AAAGGAATCA AACTGCTGCA GGCACAGAAG CTGGTGCAGT ATTTGCGGGA GTGTGAGGAT 
GTAATGGACT GGATCAATGA CAAGGAAGCA ATTGTGACGT CTGAGGAGCT GGGCCAGGAT 
TTGGAGCATG TAGAGGTACT ACAGAAGAAG TTTGAAGAGT TTCAAACTGA TCTGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTAAACCAG TTTGCTGCCA AACTTATCCA GGAGCAGCAC 
CCGGAAGAGG AGCTGATCAA GACCAAGCAG GAGGAGGTGA ATGCAGCTTG GCAGCGACTG 
AAAGGCCTGG CTCTTCAAAG GCAGGGGAAG CTCTTTGGTG CTGCCGAGGT TCAGCGCTTT 
AACAGGGATG TAGATGAGAC CATTGGTTGG ATTAAGGAGA AAGAGCAGTT AATGGCCTCT 
GATGACTTTG GCAGAGACTT AGCAAGTGTT CAAGCTCTGC TTCGGAAGCA TGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAGGACAAG GTGAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCAACAGT CACACCCTCT GAGTGCCAAC CAGATCCAGG TGAAGCGAGA GGAACTAATT 
ACCAACTGGG AGCAGATCCG AACTCTGGCC GCAGAGAGAC ATGCACGGCT TGATGACTCA 
TACAGGCTTC AGCGCTTTCT TGCTGACTTC CGTGACCTCA CGAGCTGGGT GACTGAAATG 
AAAGCCCTCA TCAATGCAGA TGAACTGGCC AATGACGTGG CTGGTGCTGA GGCCCTGCTG 
GACAGGCATC AAGAGCACAA GGGTGAAATC GATGCTCATG AAGATAGCTT TAAGTCTGCA 
GATGAGTCTG GGCAGGCCCT ACTCGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGAGCA TCCTCTCTGA GGAGAGAGCT GCCCTGCTGG AGCTGTGGGA GCTTCGGAGG 
CAACAGTATG AGCAGTGCAT GGACTTGCAG CTCTTCTACC GAGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAACAGGA GGCATTCCTG CTAAATGAAG ATTTGGGTGA CTCCTTAGAC 
AGTGTGGAAG CTCTTTTGAA GAAGCATGAG GACTTTGAGA AATCTCTCAG TGCCCAGGAA 
GAAAAAATCA CAGCACTTGA TGAGTTTGCA ACCAAGCTTA TTCAGAACAA CCACTACGCA 
ATGGAAGATG TAGCCACTCG ACGAGATGCT CTCCTGAGCC GCCGCAATGC CCTCCATGAG 
CGAGCCATGC ATCGCCGGGC ACAGCTGGCC GATTCCTTCC ACCTGCAGCA GTTCTTCCGC 
GATTCCGATG AGCTCAAAAG TTGGGTCAAT GAGAAGATGA AAACGGCCAC TGATGAAGCT 
TACAAAGATC CGTCCAACCT GCAAGGGAAA GTCCAAAAGC ACCAGGCTTT TGAGGCTGAG 
CTCTCAGCCA ACCAGAGCCG TATTGATGCC CTAGAGAAAG CTGGGCAAAA ACTAATAGAT 
GTGAACCACT ATGCCAAGGA AGAAGTAGCA GCTCGGATGA ATGAGGTCAT CAGTTTGTGG 
AAGAAACTTC TAGAGGCCAC AGAACTGAAA GGAGTCAAGC TCCGAGAAGC CAACCAGCAG 
CAACAATTTA ATCGAAATGT TGAGGACATT GAGTTGTGGC TGTATGAAGT TGAAGGTCAC 
TTGGCTTCAG ATGATTATGG TAAAGACCTC ACTAATGTCC AGAACCTCCA GAAGAAGCAT 
GCTCTGCTAG AGGCAGATGT TGCTGCTCAC CAGGATCGAA TTGACGGCAT CACAATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTC GATGCCGAAA ACATTAAAAA GAAGCAAGAG 
GCCCTTGTAG CTCGCTATGA GGCTCTCAAG GAACCCATGG TGGCCCGGAA GCAGAAGCTG 
GCAGATTCTC TTCGTCTGCA GCAGCTCTTC CGAGATGTGG AGGATGAGGA AACCTGGATT 
CGAGAAAAGG AGCCTATTGC TGCGTCCACT AACAGAGGCA AAGATCTTAT TGGAGTCCAG 
AATCTGCTAA AGAAGCACCA AGCTTTACAG GCAGAAATTG CTGGCCATGA ACCTCGCATC 
AAAGCAGTGA CACAAAAGGG CAATGCCATG GTGGAGGAAG GCCATTTTGC TGCTGAGGAT 
GTGAAGGCCA AACTGAGTGA GCTCAACCAG AAGTGGGAGG CACTGAAAGC CAAAGCCTCC 
CAGCGGAGGC AGGATCTGGA GGACTCACTA CAGGCCCAGC AGTACTTTGC CGACGCCAAT 
GAAGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATTGTGG GCAGTACCGA CTATGGGAAG 
GATGAAGACT CTGCTGAGGC TCTGCTCAAG AAGCATGAAG CTTTGATGTC CGATCTCAGT 
GCCTACGGCA GCAGCATTCA AGCTTTGCGA GAGCAGGCTC AGTCATGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGCAAGGAG CTGGTCTTGG CTCTCTATGA CTATCAAGAG 
AAGAGCCCTC GTGAGGTCAC CATGAAGAAA GGGGATATCC TCACCTTGCT CAACAGCACA 
AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGTCAGG GTTTTGTGCC AGCTGCGTAT 
GTGAAGAAGC TGGACCCCGC CCAGTCAGCC TCAAGGGAGA ACCTCCTGGA AGAACAGGGC 
AGCATTGCTC TGCGGCAGGG GCAGATCGAC AACCAGACAC GCATAACTAA GGAGGCCGGC 
AGTGTATCTC TGCGTATGAA ACAGGTGGAA GAACTGTATC AGTCTCTGCT GGAGCTGGGT 
GAGAAGAGAA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TCATGTTGTT CCGGGAAGCG 
AACGAGCTAC AGCAGTGGAT CAACGAGAAG GAAGCTGCTC TAACGAGTGA AGAGGTTGGC 
GCTGACTTGG AGCAGGTCGA GGTGCTGCAG AAGAAGTTCG ATGACTTCCA GAAGGATCTG 
AAAGCCAATG AGTCCCGGCT GAAGGACATT AACAAAGTGG CCGAGGACCT GGAGTCTGAA 
GGTCTCATGG CGGAAGAAGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG TATGATGCCC 
AGGGATGAAG CAGATTCCAA GACCGCCTCC CCATGGAAGT CTGCTCGACT GATGGTCCAC 
ACAGTGGCCA CCTTCAACTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTGCAACAG 
CTGGCTGAGG AACGTAGCCA GCTCCTGGGC AGTGCACACG AAGTACAGAG GTTCCACAGG 
GATGCGGATG AAACCAAAGA ATGGATTGAG GAGAAGAACC AGGCTCTGAA CACAGACAAC 
TATGGCCATG ATCTAGCTAG CGTCCAGGCC CTGCAGCGCA AACACGAAGG CTTCGAGAGG 
GACCTTGCAG CTCTTGGTGA CAAGGTGAAT TCCCTTGGGG AAACAGCCCA GAGGCTGATC 
CAGTCCCACC CTGAATCTGC AGAGGACTTA AAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGACCAGCC TAGGGAAGCG TGCAGACCAG CGCAAGGCCA AACTGGGTGA CTCCCATGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGAGGG 
TTGGTATCTT CAGATGAACT GGCCAAGGAT GTCACTGGAG CTGAGGCTTT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATTGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TATGCCAGCC CAGAGATCAA GGAGAAACTT 
GATATTCTTG ACCAGGAGCG CACAGACCTG GAGAAGGCCT GGGTTCAGCG CAGAATGATG 
CTGGACCACT GCCTGGAGTT GCAGCTGTTC CATCGAGACT GTGAGCAAGC AGAGAACTGG 
ATGGCTGCCC GGGAAGCCTT CCTAAACACA GAAGACAAAG GAGACTCGCT GGACAGTGTG 
GAGGCTCTGA TCAAAAAACA TGAAGACTTC GACAAAGCTA TCAATGTCCA GGAGGAGAAG 
ATAGCTGCCC TGCAGGCCTT TGCCGACCAG CTCATTGCTG TGGACCACTA TGCCAAGGGA 
GACATTGCAA ACCGACGCAA TGAGGTCCTG GACAGGTGGC GCCGCCTAAA AGCCCAGATG 
ATTGAGAAAA GGTCAAAGCT CGGAGAATCT CAAACACTTC AGCAGTTCAG CCGGGATGTA 
GATGAGATTG AAGCCTGGAT CAGTGAGAAG TTACAAACAG CCAGCGATGA GTCATACAAG 
GACCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ACCAGAAGCA CCAAGCCTTT 
GAGGCAGAAC TGCACGCCAA TGCTGACCGA ATCCGTGGAG TTATTGACAT GGGCAACTCC 
CTCATTGAGC GTGGGGCCTG TGCTGGCAGT GAGGATGCTG TCAAGGCCCG CCTGGCTGCC 
CTTGCAGACC AGTGGCAGTT CCTGGTGCAG AAGTCAGCTG AGAAGAGCCA GAAGCTGAAA 
GAGGCCAATA AGCAGCAGAA CTTCAACACC GGGATCAAAG ACTTTGACTT CTGGCTTTCT 
GAGGTGGAGG CTCTCCTGGC ATCTGAAGAC TACGGCAAAG ACCTGGCTTC CGTGAACAAC 
CTGCTCAAAA AGCATCAGCT GCTGGAGGCA GACATATCGG CCCACGAGGA TCGTCTGAAG 
GACCTGAACA GCCAGGCTGA CAGCCTGATG ACTAGCAGTG CCTTCGACAC CTCCCAAGTG 
AAAGAGAAGC GGGACACCAT CAATGGACGC TTTCAGAAGA TCAAGAGCAT GGCAACCTCC 
CGAAGAGCAA AACTGAGCGA GTCCCATCGC CTGCACCAGT TTTTCCGAGA CATGGATGAC 
GAGGAGTCCT GGATCAAGGA GAAGAAGTTG TTAGTGAGCT CTGAGGACTA TGGCAGAGAC 
CTCACTGGTG TTCAAAATCT GAGGAAGAAA CACAAGCGGC TAGAAGCCGA ACTGGCCGCA 
CACGAACCAG CCATTCAGGG TGTCCTGGAC ACGGGGAAGA AGCTGTCTGA TGACAACACC 
ATCGGGCAGG AGGAGATCCA GCAGCGTCTC GCACAGTTTG TGGAGCACTG GAAGGAACTG 
AAACAGCTAG CAGCTGCACG GGGCCAGCGG CTGGAGGAGT CCTTGGAGTA TCAGCAGTTT 
GTGGCCAACG TGGAGGAGGA GGAGGCTTGG ATCAATGAGA AGATGACCCT GGTGGCCAGC 
GAAGACTACG GGGACACTCT TGCTGCCATC CAGGGCTTAC TGAAGAAACA TGAAGCTTTT 
GAGACAGACT TCACTGTCCA CAAGGATCGA GTGAATGATG TCTGTACTAA TGGACAAGAC 
CTCATTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CAAAGATGAA GGGTCTGAAT 
GGTAAAGTGT CTGACCTGGA GAAAGCAGCA GCTCAGAGGA AAGCGAAGCT GGATGAGAAC 
TCGGCCTTCC TTCAGTTCAA TTGGAAGGCT GACGTGGTGG AGTCCTGGAT TGGTGAAAAG 
GAGAACAGCT TGAAAACAGA TGATTATGGC CGAGATCTGT CTTCTGTCCA AACTCTGCTC 
ACCAAGCAGG AGACATTTGA TGCTGGCCTG CAGGCCTTCC AGCAGGAGGG CATTGCCAAT 
ATCACTGCCC TCAAAGACCA GCTGCTAGCT GCCAAGCACA TTCAGTCGAA GGCCATCGAG 
GCCCGACATG CCTCCCTCAT GAAGAGGTGG ACCCAGCTGT TGGCCAATTC AGCTACCCGC 
AAGAAGAAGT TGCTAGAGGC CCAGAGTCAT TTCCGAAAGG TAGAAGACCT CTTCCTGACC 
TTTGCCAAAA AGGCATCGGC TTTCAACAGC TGGTTTGAGA ATGCAGAAGA GGACCTCACA 
GACCCAGTGC GCTGCAACTC TCTGGAAGAA ATCAAAGCCC TCCGAGAGGC TCATGATGCC 
TTCCGCTCAT CGCTCAGCTC TGCGCAGGCC GACTTCAACC AGCTAGCCGA GCTGGACCGT 
CAGATCAAGA GTTTCCGAGT GGCCTCCAAT CCCTACACCT GGTTCACCAT GGAGGCCCTG 
GAAGAGACGT GGAGGAACCT ACAGAAGATC ATTAAGGAGC GAGAACTGGA GCTGCAGAAG 
GAACAGCGGC GGCAGGAGGA GAATGACAAG CTACGCCAAG AGTTTGCCCA GCATGCCAAC 
GCGTTCCACC AGTGGATCCA GGAAACAAGA ACGTATCTCC TCGACGGGTC CTGCATGGTC 
GAAGAGTCGG GAACTCTGGA ATCTCAGCTT GAAGCTACCA AACGCAAGCA CCAGGAGATT 
CGGGCCATGA GAAGTCAGCT GAAGAAGATT GAGGACCTGG GGGCTGCCAT GGAGGAAGCC 
CTCATCCTGG ACAACAAGTA CACTGAGCAC AGCACTGTGG GCCTGGCCCA GCAGTGGGAC 
CAGTTAGACC AGCTGGGCAT GCGCATGCAG CACAACCTGG AGCAGCAGAT CCAGGCCAGG 
AACACAACAG GAGTCACTGA GGAGGCCCTC AAGGAGTTCA GCATGATGTT CAAACACTTC 
GACAAGGACA AGTCTGGCCG GCTGAATCAT CAAGAGTTCA AATCCTGCCT TCGTTCTCTG 
GGTTACGACC TGCCAATGGT TGAGGAAGGA GAGCCTGATC CTGAGTTTGA GGCCATACTG 
GACACTGTTG ATCCCAACAG GGACGGCCAC GTCTCCCTGC AAGAGTACAT GGCTTTCATG 
ATCAGCCGTG AAACCGAGAA TGTCAAGTCC AGTGAAGAGA TCGAGAGTGC TTTCCGGGCC 
CTCAGCTCCG AGGGCAAGCC TTATGTGACC AAGGAGGAGC TCTACCAGAA CCTGACCCGG 
GAACAAGCTG ACTACTGTGT CTCCCACATG AAGCCCTATG TGGATGGCAA GGGCCGCGAA 
CTTCCAACTG CCTTCGACTA CGTGGAGTTC ACCCGCTCTC TCTTTGTGAA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008759898.1
CDS	112..7545
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQVASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDLAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQEEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETIGWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSANQIQVKREELITNWEQIRTLAAERHARLDDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLSILSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMHRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKEEVAARMNEVISLWKKLLEATELKGVKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLSELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQGQIDNQTRITKEAGSVSLRMKQVEELYQSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGMMPRDEADSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAQRLIQSHPESAEDLKEKCTELNQAWTSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKEKLDILDQERTDLEKAWVQRRMMLDHCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAVDHYAKGDIANRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKEKRDTINGRFQKIKSMATSRRAKLSESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGQEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHIQSKAIEARHASLMKRWTQLLANSATRKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_008761676.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus spectrin, alpha, non-erythrocytic 1 (Sptan1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008761676.2

ORF Insert Sequence:

ATGGATCCAA GTGGGGTCAA AGTGCTGGAA ACAGCCGAGG ACATCCAGGA GAGACGACAG 
CAGGTTCTGG ATCGGTACCA CCGCTTCAAG GAGCTCTCTA CCTTGCGGCG GCAGAAACTG 
GAGGATTCCT ATCGCTTCCA GTTTTTTCAG AGAGATGCTG AGGAGCTGGA GAAGTGGATT 
CAGGAGAAGC TTCAAGTTGC CTCTGATGAG AACTACAAAG ACCCCACCAA CTTGCAGGGA 
AAGCTCCAGA AACACCAAGC CTTTGAAGCT GAAGTACAGG CCAACTCAGG AGCCATTGTG 
AAGCTGGATG AGACAGGAAA CTTGATGATT TCTGAAGGGC ACTTTGCATC TGAGACCATC 
CGGACACGTT TAATGGAGCT GCACCGCCAG TGGGAGTTGC TTTTGGAGAA GATGCGGGAG 
AAAGGAATCA AACTGCTGCA GGCACAGAAG CTGGTGCAGT ATTTGCGGGA GTGTGAGGAT 
GTAATGGACT GGATCAATGA CAAGGAAGCA ATTGTGACGT CTGAGGAGCT GGGCCAGGAT 
TTGGAGCATG TAGAGGTACT ACAGAAGAAG TTTGAAGAGT TTCAAACTGA TCTGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTAAACCAG TTTGCTGCCA AACTTATCCA GGAGCAGCAC 
CCGGAAGAGG AGCTGATCAA GACCAAGCAG GAGGAGGTGA ATGCAGCTTG GCAGCGACTG 
AAAGGCCTGG CTCTTCAAAG GCAGGGGAAG CTCTTTGGTG CTGCCGAGGT TCAGCGCTTT 
AACAGGGATG TAGATGAGAC CATTGGTTGG ATTAAGGAGA AAGAGCAGTT AATGGCCTCT 
GATGACTTTG GCAGAGACTT AGCAAGTGTT CAAGCTCTGC TTCGGAAGCA TGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAGGACAAG GTGAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCAACAGT CACACCCTCT GAGTGCCAAC CAGATCCAGG TGAAGCGAGA GGAACTAATT 
ACCAACTGGG AGCAGATCCG AACTCTGGCC GCAGAGAGAC ATGCACGGCT TGATGACTCA 
TACAGGCTTC AGCGCTTTCT TGCTGACTTC CGTGACCTCA CGAGCTGGGT GACTGAAATG 
AAAGCCCTCA TCAATGCAGA TGAACTGGCC AATGACGTGG CTGGTGCTGA GGCCCTGCTG 
GACAGGCATC AAGAGCACAA GGGTGAAATC GATGCTCATG AAGATAGCTT TAAGTCTGCA 
GATGAGTCTG GGCAGGCCCT ACTCGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGAGCA TCCTCTCTGA GGAGAGAGCT GCCCTGCTGG AGCTGTGGGA GCTTCGGAGG 
CAACAGTATG AGCAGTGCAT GGACTTGCAG CTCTTCTACC GAGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAACAGGA GGCATTCCTG CTAAATGAAG ATTTGGGTGA CTCCTTAGAC 
AGTGTGGAAG CTCTTTTGAA GAAGCATGAG GACTTTGAGA AATCTCTCAG TGCCCAGGAA 
GAAAAAATCA CAGCACTTGA TGAGTTTGCA ACCAAGCTTA TTCAGAACAA CCACTACGCA 
ATGGAAGATG TAGCCACTCG ACGAGATGCT CTCCTGAGCC GCCGCAATGC CCTCCATGAG 
CGAGCCATGC ATCGCCGGGC ACAGCTGGCC GATTCCTTCC ACCTGCAGCA GTTCTTCCGC 
GATTCCGATG AGCTCAAAAG TTGGGTCAAT GAGAAGATGA AAACGGCCAC TGATGAAGCT 
TACAAAGATC CGTCCAACCT GCAAGGGAAA GTCCAAAAGC ACCAGGCTTT TGAGGCTGAG 
CTCTCAGCCA ACCAGAGCCG TATTGATGCC CTAGAGAAAG CTGGGCAAAA ACTAATAGAT 
GTGAACCACT ATGCCAAGGA AGAAGTAGCA GCTCGGATGA ATGAGGTCAT CAGTTTGTGG 
AAGAAACTTC TAGAGGCCAC AGAACTGAAA GGAGTCAAGC TCCGAGAAGC CAACCAGCAG 
CAACAATTTA ATCGAAATGT TGAGGACATT GAGTTGTGGC TGTATGAAGT TGAAGGTCAC 
TTGGCTTCAG ATGATTATGG TAAAGACCTC ACTAATGTCC AGAACCTCCA GAAGAAGCAT 
GCTCTGCTAG AGGCAGATGT TGCTGCTCAC CAGGATCGAA TTGACGGCAT CACAATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTC GATGCCGAAA ACATTAAAAA GAAGCAAGAG 
GCCCTTGTAG CTCGCTATGA GGCTCTCAAG GAACCCATGG TGGCCCGGAA GCAGAAGCTG 
GCAGATTCTC TTCGTCTGCA GCAGCTCTTC CGAGATGTGG AGGATGAGGA AACCTGGATT 
CGAGAAAAGG AGCCTATTGC TGCGTCCACT AACAGAGGCA AAGATCTTAT TGGAGTCCAG 
AATCTGCTAA AGAAGCACCA AGCTTTACAG GCAGAAATTG CTGGCCATGA ACCTCGCATC 
AAAGCAGTGA CACAAAAGGG CAATGCCATG GTGGAGGAAG GCCATTTTGC TGCTGAGGAT 
GTGAAGGCCA AACTGAGTGA GCTCAACCAG AAGTGGGAGG CACTGAAAGC CAAAGCCTCC 
CAGCGGAGGC AGGATCTGGA GGACTCACTA CAGGCCCAGC AGTACTTTGC CGACGCCAAT 
GAAGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATTGTGG GCAGTACCGA CTATGGGAAG 
GATGAAGACT CTGCTGAGGC TCTGCTCAAG AAGCATGAAG CTTTGATGTC CGATCTCAGT 
GCCTACGGCA GCAGCATTCA AGCTTTGCGA GAGCAGGCTC AGTCATGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGCAAGGAG CTGGTCTTGG CTCTCTATGA CTATCAAGAG 
AAGAGCCCTC GTGAGGTCAC CATGAAGAAA GGGGATATCC TCACCTTGCT CAACAGCACA 
AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGTCAGG GTTTTGTGCC AGCTGCGTAT 
GTGAAGAAGC TGGACCCCGC CCAGTCAGCC TCAAGGGAGA ACCTCCTGGA AGAACAGGGC 
AGCATTGCTC TGCGGCAGGG GCAGATCGAC AACCAGACAC GCATAACTAA GGAGGCCGGC 
AGTGTATCTC TGCGTATGAA ACAGGTGGAA GAACTGTATC AGTCTCTGCT GGAGCTGGGT 
GAGAAGAGAA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TCATGTTGTT CCGGGAAGCG 
AACGAGCTAC AGCAGTGGAT CAACGAGAAG GAAGCTGCTC TAACGAGTGA AGAGGTTGGC 
GCTGACTTGG AGCAGGTCGA GGTGCTGCAG AAGAAGTTCG ATGACTTCCA GAAGGATCTG 
AAAGCCAATG AGTCCCGGCT GAAGGACATT AACAAAGTGG CCGAGGACCT GGAGTCTGAA 
GGTCTCATGG CGGAAGAAGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG TATGATGCCC 
AGGGATGAAG CAGATTCCAA GACCGCCTCC CCATGGAAGT CTGCTCGACT GATGGTCCAC 
ACAGTGGCCA CCTTCAACTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTGCAACAG 
CTGGCTGAGG AACGTAGCCA GCTCCTGGGC AGTGCACACG AAGTACAGAG GTTCCACAGG 
GATGCGGATG AAACCAAAGA ATGGATTGAG GAGAAGAACC AGGCTCTGAA CACAGACAAC 
TATGGCCATG ATCTAGCTAG CGTCCAGGCC CTGCAGCGCA AACACGAAGG CTTCGAGAGG 
GACCTTGCAG CTCTTGGTGA CAAGGTGAAT TCCCTTGGGG AAACAGCCCA GAGGCTGATC 
CAGTCCCACC CTGAATCTGC AGAGGACTTA AAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGACCAGCC TAGGGAAGCG TGCAGACCAG CGCAAGGCCA AACTGGGTGA CTCCCATGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGAGGG 
TTGGTATCTT CAGATGAACT GGCCAAGGAT GTCACTGGAG CTGAGGCTTT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATTGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TATGCCAGCC CAGAGATCAA GGAGAAACTT 
GATATTCTTG ACCAGGAGCG CACAGACCTG GAGAAGGCCT GGGTTCAGCG CAGAATGATG 
CTGGACCACT GCCTGGAGTT GCAGCTGTTC CATCGAGACT GTGAGCAAGC AGAGAACTGG 
ATGGCTGCCC GGGAAGCCTT CCTAAACACA GAAGACAAAG GAGACTCGCT GGACAGTGTG 
GAGGCTCTGA TCAAAAAACA TGAAGACTTC GACAAAGCTA TCAATGTCCA GGAGGAGAAG 
ATAGCTGCCC TGCAGGCCTT TGCCGACCAG CTCATTGCTG TGGACCACTA TGCCAAGGGA 
GACATTGCAA ACCGACGCAA TGAGGTCCTG GACAGGTGGC GCCGCCTAAA AGCCCAGATG 
ATTGAGAAAA GGTCAAAGCT CGGAGAATCT CAAACACTTC AGCAGTTCAG CCGGGATGTA 
GATGAGATTG AAGCCTGGAT CAGTGAGAAG TTACAAACAG CCAGCGATGA GTCATACAAG 
GACCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ACCAGAAGCA CCAAGCCTTT 
GAGGCAGAAC TGCACGCCAA TGCTGACCGA ATCCGTGGAG TTATTGACAT GGGCAACTCC 
CTCATTGAGC GTGGGGCCTG TGCTGGCAGT GAGGATGCTG TCAAGGCCCG CCTGGCTGCC 
CTTGCAGACC AGTGGCAGTT CCTGGTGCAG AAGTCAGCTG AGAAGAGCCA GAAGCTGAAA 
GAGGCCAATA AGCAGCAGAA CTTCAACACC GGGATCAAAG ACTTTGACTT CTGGCTTTCT 
GAGGTGGAGG CTCTCCTGGC ATCTGAAGAC TACGGCAAAG ACCTGGCTTC CGTGAACAAC 
CTGCTCAAAA AGCATCAGCT GCTGGAGGCA GACATATCGG CCCACGAGGA TCGTCTGAAG 
GACCTGAACA GCCAGGCTGA CAGCCTGATG ACTAGCAGTG CCTTCGACAC CTCCCAAGTG 
AAAGAGAAGC GGGACACCAT CAATGGACGC TTTCAGAAGA TCAAGAGCAT GGCAACCTCC 
CGAAGAGCAA AACTGAGCGA GTCCCATCGC CTGCACCAGT TTTTCCGAGA CATGGATGAC 
GAGGAGTCCT GGATCAAGGA GAAGAAGTTG TTAGTGAGCT CTGAGGACTA TGGCAGAGAC 
CTCACTGGTG TTCAAAATCT GAGGAAGAAA CACAAGCGGC TAGAAGCCGA ACTGGCCGCA 
CACGAACCAG CCATTCAGGG TGTCCTGGAC ACGGGGAAGA AGCTGTCTGA TGACAACACC 
ATCGGGCAGG AGGAGATCCA GCAGCGTCTC GCACAGTTTG TGGAGCACTG GAAGGAACTG 
AAACAGCTAG CAGCTGCACG GGGCCAGCGG CTGGAGGAGT CCTTGGAGTA TCAGCAGTTT 
GTGGCCAACG TGGAGGAGGA GGAGGCTTGG ATCAATGAGA AGATGACCCT GGTGGCCAGC 
GAAGACTACG GGGACACTCT TGCTGCCATC CAGGGCTTAC TGAAGAAACA TGAAGCTTTT 
GAGACAGACT TCACTGTCCA CAAGGATCGA GTGAATGATG TCTGTACTAA TGGACAAGAC 
CTCATTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CAAAGATGAA GGGTCTGAAT 
GGTAAAGTGT CTGACCTGGA GAAAGCAGCA GCTCAGAGGA AAGCGAAGCT GGATGAGAAC 
TCGGCCTTCC TTCAGTTCAA TTGGAAGGCT GACGTGGTGG AGTCCTGGAT TGGTGAAAAG 
GAGAACAGCT TGAAAACAGA TGATTATGGC CGAGATCTGT CTTCTGTCCA AACTCTGCTC 
ACCAAGCAGG AGACATTTGA TGCTGGCCTG CAGGCCTTCC AGCAGGAGGG CATTGCCAAT 
ATCACTGCCC TCAAAGACCA GCTGCTAGCT GCCAAGCACA TTCAGTCGAA GGCCATCGAG 
GCCCGACATG CCTCCCTCAT GAAGAGGTGG ACCCAGCTGT TGGCCAATTC AGCTACCCGC 
AAGAAGAAGT TGCTAGAGGC CCAGAGTCAT TTCCGAAAGG TAGAAGACCT CTTCCTGACC 
TTTGCCAAAA AGGCATCGGC TTTCAACAGC TGGTTTGAGA ATGCAGAAGA GGACCTCACA 
GACCCAGTGC GCTGCAACTC TCTGGAAGAA ATCAAAGCCC TCCGAGAGGC TCATGATGCC 
TTCCGCTCAT CGCTCAGCTC TGCGCAGGCC GACTTCAACC AGCTAGCCGA GCTGGACCGT 
CAGATCAAGA GTTTCCGAGT GGCCTCCAAT CCCTACACCT GGTTCACCAT GGAGGCCCTG 
GAAGAGACGT GGAGGAACCT ACAGAAGATC ATTAAGGAGC GAGAACTGGA GCTGCAGAAG 
GAACAGCGGC GGCAGGAGGA GAATGACAAG CTACGCCAAG AGTTTGCCCA GCATGCCAAC 
GCGTTCCACC AGTGGATCCA GGAAACAAGA ACGTATCTCC TCGACGGGTC CTGCATGGTC 
GAAGAGTCGG GAACTCTGGA ATCTCAGCTT GAAGCTACCA AACGCAAGCA CCAGGAGATT 
CGGGCCATGA GAAGTCAGCT GAAGAAGATT GAGGACCTGG GGGCTGCCAT GGAGGAAGCC 
CTCATCCTGG ACAACAAGTA CACTGAGCAC AGCACTGTGG GCCTGGCCCA GCAGTGGGAC 
CAGTTAGACC AGCTGGGCAT GCGCATGCAG CACAACCTGG AGCAGCAGAT CCAGGCCAGG 
AACACAACAG GAGTCACTGA GGAGGCCCTC AAGGAGTTCA GCATGATGTT CAAACACTTC 
GACAAGGACA AGTCTGGCCG GCTGAATCAT CAAGAGTTCA AATCCTGCCT TCGTTCTCTG 
GGTTACGACC TGCCAATGGT TGAGGAAGGA GAGCCTGATC CTGAGTTTGA GGCCATACTG 
GACACTGTTG ATCCCAACAG GGACGGCCAC GTCTCCCTGC AAGAGTACAT GGCTTTCATG 
ATCAGCCGTG AAACCGAGAA TGTCAAGTCC AGTGAAGAGA TCGAGAGTGC TTTCCGGGCC 
CTCAGCTCCG AGGGCAAGCC TTATGTGACC AAGGAGGAGC TCTACCAGAA CCTGACCCGG 
GAACAAGCTG ACTACTGTGT CTCCCACATG AAGCCCTATG TGGATGGCAA GGGCCGCGAA 
CTTCCAACTG CCTTCGACTA CGTGGAGTTC ACCCGCTCTC TCTTTGTGAA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sptan1 ( XM_008761677.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008759899.1 Rattus norvegicus spectrin, alpha, non-erythrocytic 1 (Sptan1), transcript variant X5, mRNA.

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CloneID	ORa43564
Clone ID Related Accession (Same CDS sequence)	XM_008761677.2
Accession Version	XM_008761677.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008761677.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCAA GTGGGGTCAA AGTGCTGGAA ACAGCCGAGG ACATCCAGGA GAGACGACAG 
CAGGTTCTGG ATCGGTACCA CCGCTTCAAG GAGCTCTCTA CCTTGCGGCG GCAGAAACTG 
GAGGATTCCT ATCGCTTCCA GTTTTTTCAG AGAGATGCTG AGGAGCTGGA GAAGTGGATT 
CAGGAGAAGC TTCAAGTTGC CTCTGATGAG AACTACAAAG ACCCCACCAA CTTGCAGGGA 
AAGCTCCAGA AACACCAAGC CTTTGAAGCT GAAGTACAGG CCAACTCAGG AGCCATTGTG 
AAGCTGGATG AGACAGGAAA CTTGATGATT TCTGAAGGGC ACTTTGCATC TGAGACCATC 
CGGACACGTT TAATGGAGCT GCACCGCCAG TGGGAGTTGC TTTTGGAGAA GATGCGGGAG 
AAAGGAATCA AACTGCTGCA GGCACAGAAG CTGGTGCAGT ATTTGCGGGA GTGTGAGGAT 
GTAATGGACT GGATCAATGA CAAGGAAGCA ATTGTGACGT CTGAGGAGCT GGGCCAGGAT 
TTGGAGCATG TAGAGGTACT ACAGAAGAAG TTTGAAGAGT TTCAAACTGA TCTGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTAAACCAG TTTGCTGCCA AACTTATCCA GGAGCAGCAC 
CCGGAAGAGG AGCTGATCAA GACCAAGCAG GAGGAGGTGA ATGCAGCTTG GCAGCGACTG 
AAAGGCCTGG CTCTTCAAAG GCAGGGGAAG CTCTTTGGTG CTGCCGAGGT TCAGCGCTTT 
AACAGGGATG TAGATGAGAC CATTGGTTGG ATTAAGGAGA AAGAGCAGTT AATGGCCTCT 
GATGACTTTG GCAGAGACTT AGCAAGTGTT CAAGCTCTGC TTCGGAAGCA TGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAGGACAAG GTGAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCAACAGT CACACCCTCT GAGTGCCAAC CAGATCCAGG TGAAGCGAGA GGAACTAATT 
ACCAACTGGG AGCAGATCCG AACTCTGGCC GCAGAGAGAC ATGCACGGCT TGATGACTCA 
TACAGGCTTC AGCGCTTTCT TGCTGACTTC CGTGACCTCA CGAGCTGGGT GACTGAAATG 
AAAGCCCTCA TCAATGCAGA TGAACTGGCC AATGACGTGG CTGGTGCTGA GGCCCTGCTG 
GACAGGCATC AAGAGCACAA GGGTGAAATC GATGCTCATG AAGATAGCTT TAAGTCTGCA 
GATGAGTCTG GGCAGGCCCT ACTCGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGAGCA TCCTCTCTGA GGAGAGAGCT GCCCTGCTGG AGCTGTGGGA GCTTCGGAGG 
CAACAGTATG AGCAGTGCAT GGACTTGCAG CTCTTCTACC GAGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAACAGGA GGCATTCCTG CTAAATGAAG ATTTGGGTGA CTCCTTAGAC 
AGTGTGGAAG CTCTTTTGAA GAAGCATGAG GACTTTGAGA AATCTCTCAG TGCCCAGGAA 
GAAAAAATCA CAGCACTTGA TGAGTTTGCA ACCAAGCTTA TTCAGAACAA CCACTACGCA 
ATGGAAGATG TAGCCACTCG ACGAGATGCT CTCCTGAGCC GCCGCAATGC CCTCCATGAG 
CGAGCCATGC ATCGCCGGGC ACAGCTGGCC GATTCCTTCC ACCTGCAGCA GTTCTTCCGC 
GATTCCGATG AGCTCAAAAG TTGGGTCAAT GAGAAGATGA AAACGGCCAC TGATGAAGCT 
TACAAAGATC CGTCCAACCT GCAAGGGAAA GTCCAAAAGC ACCAGGCTTT TGAGGCTGAG 
CTCTCAGCCA ACCAGAGCCG TATTGATGCC CTAGAGAAAG CTGGGCAAAA ACTAATAGAT 
GTGAACCACT ATGCCAAGGA AGAAGTAGCA GCTCGGATGA ATGAGGTCAT CAGTTTGTGG 
AAGAAACTTC TAGAGGCCAC AGAACTGAAA GGAGTCAAGC TCCGAGAAGC CAACCAGCAG 
CAACAATTTA ATCGAAATGT TGAGGACATT GAGTTGTGGC TGTATGAAGT TGAAGGTCAC 
TTGGCTTCAG ATGATTATGG TAAAGACCTC ACTAATGTCC AGAACCTCCA GAAGAAGCAT 
GCTCTGCTAG AGGCAGATGT TGCTGCTCAC CAGGATCGAA TTGACGGCAT CACAATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTC GATGCCGAAA ACATTAAAAA GAAGCAAGAG 
GCCCTTGTAG CTCGCTATGA GGCTCTCAAG GAACCCATGG TGGCCCGGAA GCAGAAGCTG 
GCAGATTCTC TTCGTCTGCA GCAGCTCTTC CGAGATGTGG AGGATGAGGA AACCTGGATT 
CGAGAAAAGG AGCCTATTGC TGCGTCCACT AACAGAGGCA AAGATCTTAT TGGAGTCCAG 
AATCTGCTAA AGAAGCACCA AGCTTTACAG GCAGAAATTG CTGGCCATGA ACCTCGCATC 
AAAGCAGTGA CACAAAAGGG CAATGCCATG GTGGAGGAAG GCCATTTTGC TGCTGAGGAT 
GTGAAGGCCA AACTGAGTGA GCTCAACCAG AAGTGGGAGG CACTGAAAGC CAAAGCCTCC 
CAGCGGAGGC AGGATCTGGA GGACTCACTA CAGGCCCAGC AGTACTTTGC CGACGCCAAT 
GAAGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATTGTGG GCAGTACCGA CTATGGGAAG 
GATGAAGACT CTGCTGAGGC TCTGCTCAAG AAGCATGAAG CTTTGATGTC CGATCTCAGT 
GCCTACGGCA GCAGCATTCA AGCTTTGCGA GAGCAGGCTC AGTCATGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGCAAGGAG CTGGTCTTGG CTCTCTATGA CTATCAAGAG 
AAGAGCCCTC GTGAGGTCAC CATGAAGAAA GGGGATATCC TCACCTTGCT CAACAGCACA 
AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGTCAGG GTTTTGTGCC AGCTGCGTAT 
GTGAAGAAGC TGGACCCCGC CCAGTCAGCC TCAAGGGAGA ACCTCCTGGA AGAACAGGGC 
AGCATTGCTC TGCGGCAGGG GCAGATCGAC AACCAGTATC AGTCTCTGCT GGAGCTGGGT 
GAGAAGAGAA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TCATGTTGTT CCGGGAAGCG 
AACGAGCTAC AGCAGTGGAT CAACGAGAAG GAAGCTGCTC TAACGAGTGA AGAGGTTGGC 
GCTGACTTGG AGCAGGTCGA GGTGCTGCAG AAGAAGTTCG ATGACTTCCA GAAGGATCTG 
AAAGCCAATG AGTCCCGGCT GAAGGACATT AACAAAGTGG CCGAGGACCT GGAGTCTGAA 
GGTCTCATGG CGGAAGAAGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG TATGATGCCC 
AGGGATGAAG CAGATTCCAA GACCGCCTCC CCATGGAAGT CTGCTCGACT GATGGTCCAC 
ACAGTGGCCA CCTTCAACTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTGCAACAG 
CTGGCTGAGG AACGTAGCCA GCTCCTGGGC AGTGCACACG AAGTACAGAG GTTCCACAGG 
GATGCGGATG AAACCAAAGA ATGGATTGAG GAGAAGAACC AGGCTCTGAA CACAGACAAC 
TATGGCCATG ATCTAGCTAG CGTCCAGGCC CTGCAGCGCA AACACGAAGG CTTCGAGAGG 
GACCTTGCAG CTCTTGGTGA CAAGGTGAAT TCCCTTGGGG AAACAGCCCA GAGGCTGATC 
CAGTCCCACC CTGAATCTGC AGAGGACTTA AAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGACCAGCC TAGGGAAGCG TGCAGACCAG CGCAAGGCCA AACTGGGTGA CTCCCATGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGAGGG 
TTGGTATCTT CAGATGAACT GGCCAAGGAT GTCACTGGAG CTGAGGCTTT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATTGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TATGCCAGCC CAGAGATCAA GGAGAAACTT 
GATATTCTTG ACCAGGAGCG CACAGACCTG GAGAAGGCCT GGGTTCAGCG CAGAATGATG 
CTGGACCACT GCCTGGAGTT GCAGCTGTTC CATCGAGACT GTGAGCAAGC AGAGAACTGG 
ATGGCTGCCC GGGAAGCCTT CCTAAACACA GAAGACAAAG GAGACTCGCT GGACAGTGTG 
GAGGCTCTGA TCAAAAAACA TGAAGACTTC GACAAAGCTA TCAATGTCCA GGAGGAGAAG 
ATAGCTGCCC TGCAGGCCTT TGCCGACCAG CTCATTGCTG TGGACCACTA TGCCAAGGGA 
GACATTGCAA ACCGACGCAA TGAGGTCCTG GACAGGTGGC GCCGCCTAAA AGCCCAGATG 
ATTGAGAAAA GGTCAAAGCT CGGAGAATCT CAAACACTTC AGCAGTTCAG CCGGGATGTA 
GATGAGATTG AAGCCTGGAT CAGTGAGAAG TTACAAACAG CCAGCGATGA GTCATACAAG 
GACCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ACCAGAAGCA CCAAGCCTTT 
GAGGCAGAAC TGCACGCCAA TGCTGACCGA ATCCGTGGAG TTATTGACAT GGGCAACTCC 
CTCATTGAGC GTGGGGCCTG TGCTGGCAGT GAGGATGCTG TCAAGGCCCG CCTGGCTGCC 
CTTGCAGACC AGTGGCAGTT CCTGGTGCAG AAGTCAGCTG AGAAGAGCCA GAAGCTGAAA 
GAGGCCAATA AGCAGCAGAA CTTCAACACC GGGATCAAAG ACTTTGACTT CTGGCTTTCT 
GAGGTGGAGG CTCTCCTGGC ATCTGAAGAC TACGGCAAAG ACCTGGCTTC CGTGAACAAC 
CTGCTCAAAA AGCATCAGCT GCTGGAGGCA GACATATCGG CCCACGAGGA TCGTCTGAAG 
GACCTGAACA GCCAGGCTGA CAGCCTGATG ACTAGCAGTG CCTTCGACAC CTCCCAAGTG 
AAAGAGAAGC GGGACACCAT CAATGGACGC TTTCAGAAGA TCAAGAGCAT GGCAACCTCC 
CGAAGAGCAA AACTGAGCGA GTCCCATCGC CTGCACCAGT TTTTCCGAGA CATGGATGAC 
GAGGAGTCCT GGATCAAGGA GAAGAAGTTG TTAGTGAGCT CTGAGGACTA TGGCAGAGAC 
CTCACTGGTG TTCAAAATCT GAGGAAGAAA CACAAGCGGC TAGAAGCCGA ACTGGCCGCA 
CACGAACCAG CCATTCAGGG TGTCCTGGAC ACGGGGAAGA AGCTGTCTGA TGACAACACC 
ATCGGGCAGG AGGAGATCCA GCAGCGTCTC GCACAGTTTG TGGAGCACTG GAAGGAACTG 
AAACAGCTAG CAGCTGCACG GGGCCAGCGG CTGGAGGAGT CCTTGGAGTA TCAGCAGTTT 
GTGGCCAACG TGGAGGAGGA GGAGGCTTGG ATCAATGAGA AGATGACCCT GGTGGCCAGC 
GAAGACTACG GGGACACTCT TGCTGCCATC CAGGGCTTAC TGAAGAAACA TGAAGCTTTT 
GAGACAGACT TCACTGTCCA CAAGGATCGA GTGAATGATG TCTGTACTAA TGGACAAGAC 
CTCATTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CAAAGATGAA GGGTCTGAAT 
GGTAAAGTGT CTGACCTGGA GAAAGCAGCA GCTCAGAGGA AAGCGAAGCT GGATGAGAAC 
TCGGCCTTCC TTCAGTTCAA TTGGAAGGCT GACGTGGTGG AGTCCTGGAT TGGTGAAAAG 
GAGAACAGCT TGAAAACAGA TGATTATGGC CGAGATCTGT CTTCTGTCCA AACTCTGCTC 
ACCAAGCAGG AGACATTTGA TGCTGGCCTG CAGGCCTTCC AGCAGGAGGG CATTGCCAAT 
ATCACTGCCC TCAAAGACCA GCTGCTAGCT GCCAAGCACA TTCAGTCGAA GGCCATCGAG 
GCCCGACATG CCTCCCTCAT GAAGAGGTGG ACCCAGCTGT TGGCCAATTC AGCTACCCGC 
AAGAAGAAGT TGCTAGAGGC CCAGAGTCAT TTCCGAAAGG TAGAAGACCT CTTCCTGACC 
TTTGCCAAAA AGGCATCGGC TTTCAACAGC TGGTTTGAGA ATGCAGAAGA GGACCTCACA 
GACCCAGTGC GCTGCAACTC TCTGGAAGAA ATCAAAGCCC TCCGAGAGGC TCATGATGCC 
TTCCGCTCAT CGCTCAGCTC TGCGCAGGCC GACTTCAACC AGCTAGCCGA GCTGGACCGT 
CAGATCAAGA GTTTCCGAGT GGCCTCCAAT CCCTACACCT GGTTCACCAT GGAGGCCCTG 
GAAGAGACGT GGAGGAACCT ACAGAAGATC ATTAAGGAGC GAGAACTGGA GCTGCAGAAG 
GAACAGCGGC GGCAGGAGGA GAATGACAAG CTACGCCAAG AGTTTGCCCA GCATGCCAAC 
GCGTTCCACC AGTGGATCCA GGAAACAAGA ACGTATCTCC TCGACGGGTC CTGCATGGTC 
GAAGAGTCGG GAACTCTGGA ATCTCAGCTT GAAGCTACCA AACGCAAGCA CCAGGAGATT 
CGGGCCATGA GAAGTCAGCT GAAGAAGATT GAGGACCTGG GGGCTGCCAT GGAGGAAGCC 
CTCATCCTGG ACAACAAGTA CACTGAGCAC AGCACTGTGG GCCTGGCCCA GCAGTGGGAC 
CAGTTAGACC AGCTGGGCAT GCGCATGCAG CACAACCTGG AGCAGCAGAT CCAGGCCAGG 
AACACAACAG GAGTCACTGA GGAGGCCCTC AAGGAGTTCA GCATGATGTT CAAACACTTC 
GACAAGGACA AGTCTGGCCG GCTGAATCAT CAAGAGTTCA AATCCTGCCT TCGTTCTCTG 
GGTTACGACC TGCCAATGGT TGAGGAAGGA GAGCCTGATC CTGAGTTTGA GGCCATACTG 
GACACTGTTG ATCCCAACAG GGACGGCCAC GTCTCCCTGC AAGAGTACAT GGCTTTCATG 
ATCAGCCGTG AAACCGAGAA TGTCAAGTCC AGTGAAGAGA TCGAGAGTGC TTTCCGGGCC 
CTCAGCTCCG AGGGCAAGCC TTATGTGACC AAGGAGGAGC TCTACCAGAA CCTGACCCGG 
GAACAAGCTG ACTACTGTGT CTCCCACATG AAGCCCTATG TGGATGGCAA GGGCCGCGAA 
CTTCCAACTG CCTTCGACTA CGTGGAGTTC ACCCGCTCTC TCTTTGTGAA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008759899.1
CDS	112..7485
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQVASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDLAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQEEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETIGWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSANQIQVKREELITNWEQIRTLAAERHARLDDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLSILSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMHRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKEEVAARMNEVISLWKKLLEATELKGVKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLSELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQGQIDNQYQSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGMMPRDEADSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAQRLIQSHPESAEDLKEKCTELNQAWTSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKEKLDILDQERTDLEKAWVQRRMMLDHCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAVDHYAKGDIANRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKEKRDTINGRFQKIKSMATSRRAKLSESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGQEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHIQSKAIEARHASLMKRWTQLLANSATRKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_008761677.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus spectrin, alpha, non-erythrocytic 1 (Sptan1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008761677.2

ORF Insert Sequence:

ATGGATCCAA GTGGGGTCAA AGTGCTGGAA ACAGCCGAGG ACATCCAGGA GAGACGACAG 
CAGGTTCTGG ATCGGTACCA CCGCTTCAAG GAGCTCTCTA CCTTGCGGCG GCAGAAACTG 
GAGGATTCCT ATCGCTTCCA GTTTTTTCAG AGAGATGCTG AGGAGCTGGA GAAGTGGATT 
CAGGAGAAGC TTCAAGTTGC CTCTGATGAG AACTACAAAG ACCCCACCAA CTTGCAGGGA 
AAGCTCCAGA AACACCAAGC CTTTGAAGCT GAAGTACAGG CCAACTCAGG AGCCATTGTG 
AAGCTGGATG AGACAGGAAA CTTGATGATT TCTGAAGGGC ACTTTGCATC TGAGACCATC 
CGGACACGTT TAATGGAGCT GCACCGCCAG TGGGAGTTGC TTTTGGAGAA GATGCGGGAG 
AAAGGAATCA AACTGCTGCA GGCACAGAAG CTGGTGCAGT ATTTGCGGGA GTGTGAGGAT 
GTAATGGACT GGATCAATGA CAAGGAAGCA ATTGTGACGT CTGAGGAGCT GGGCCAGGAT 
TTGGAGCATG TAGAGGTACT ACAGAAGAAG TTTGAAGAGT TTCAAACTGA TCTGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTAAACCAG TTTGCTGCCA AACTTATCCA GGAGCAGCAC 
CCGGAAGAGG AGCTGATCAA GACCAAGCAG GAGGAGGTGA ATGCAGCTTG GCAGCGACTG 
AAAGGCCTGG CTCTTCAAAG GCAGGGGAAG CTCTTTGGTG CTGCCGAGGT TCAGCGCTTT 
AACAGGGATG TAGATGAGAC CATTGGTTGG ATTAAGGAGA AAGAGCAGTT AATGGCCTCT 
GATGACTTTG GCAGAGACTT AGCAAGTGTT CAAGCTCTGC TTCGGAAGCA TGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAGGACAAG GTGAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCAACAGT CACACCCTCT GAGTGCCAAC CAGATCCAGG TGAAGCGAGA GGAACTAATT 
ACCAACTGGG AGCAGATCCG AACTCTGGCC GCAGAGAGAC ATGCACGGCT TGATGACTCA 
TACAGGCTTC AGCGCTTTCT TGCTGACTTC CGTGACCTCA CGAGCTGGGT GACTGAAATG 
AAAGCCCTCA TCAATGCAGA TGAACTGGCC AATGACGTGG CTGGTGCTGA GGCCCTGCTG 
GACAGGCATC AAGAGCACAA GGGTGAAATC GATGCTCATG AAGATAGCTT TAAGTCTGCA 
GATGAGTCTG GGCAGGCCCT ACTCGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGAGCA TCCTCTCTGA GGAGAGAGCT GCCCTGCTGG AGCTGTGGGA GCTTCGGAGG 
CAACAGTATG AGCAGTGCAT GGACTTGCAG CTCTTCTACC GAGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAACAGGA GGCATTCCTG CTAAATGAAG ATTTGGGTGA CTCCTTAGAC 
AGTGTGGAAG CTCTTTTGAA GAAGCATGAG GACTTTGAGA AATCTCTCAG TGCCCAGGAA 
GAAAAAATCA CAGCACTTGA TGAGTTTGCA ACCAAGCTTA TTCAGAACAA CCACTACGCA 
ATGGAAGATG TAGCCACTCG ACGAGATGCT CTCCTGAGCC GCCGCAATGC CCTCCATGAG 
CGAGCCATGC ATCGCCGGGC ACAGCTGGCC GATTCCTTCC ACCTGCAGCA GTTCTTCCGC 
GATTCCGATG AGCTCAAAAG TTGGGTCAAT GAGAAGATGA AAACGGCCAC TGATGAAGCT 
TACAAAGATC CGTCCAACCT GCAAGGGAAA GTCCAAAAGC ACCAGGCTTT TGAGGCTGAG 
CTCTCAGCCA ACCAGAGCCG TATTGATGCC CTAGAGAAAG CTGGGCAAAA ACTAATAGAT 
GTGAACCACT ATGCCAAGGA AGAAGTAGCA GCTCGGATGA ATGAGGTCAT CAGTTTGTGG 
AAGAAACTTC TAGAGGCCAC AGAACTGAAA GGAGTCAAGC TCCGAGAAGC CAACCAGCAG 
CAACAATTTA ATCGAAATGT TGAGGACATT GAGTTGTGGC TGTATGAAGT TGAAGGTCAC 
TTGGCTTCAG ATGATTATGG TAAAGACCTC ACTAATGTCC AGAACCTCCA GAAGAAGCAT 
GCTCTGCTAG AGGCAGATGT TGCTGCTCAC CAGGATCGAA TTGACGGCAT CACAATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTC GATGCCGAAA ACATTAAAAA GAAGCAAGAG 
GCCCTTGTAG CTCGCTATGA GGCTCTCAAG GAACCCATGG TGGCCCGGAA GCAGAAGCTG 
GCAGATTCTC TTCGTCTGCA GCAGCTCTTC CGAGATGTGG AGGATGAGGA AACCTGGATT 
CGAGAAAAGG AGCCTATTGC TGCGTCCACT AACAGAGGCA AAGATCTTAT TGGAGTCCAG 
AATCTGCTAA AGAAGCACCA AGCTTTACAG GCAGAAATTG CTGGCCATGA ACCTCGCATC 
AAAGCAGTGA CACAAAAGGG CAATGCCATG GTGGAGGAAG GCCATTTTGC TGCTGAGGAT 
GTGAAGGCCA AACTGAGTGA GCTCAACCAG AAGTGGGAGG CACTGAAAGC CAAAGCCTCC 
CAGCGGAGGC AGGATCTGGA GGACTCACTA CAGGCCCAGC AGTACTTTGC CGACGCCAAT 
GAAGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATTGTGG GCAGTACCGA CTATGGGAAG 
GATGAAGACT CTGCTGAGGC TCTGCTCAAG AAGCATGAAG CTTTGATGTC CGATCTCAGT 
GCCTACGGCA GCAGCATTCA AGCTTTGCGA GAGCAGGCTC AGTCATGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGCAAGGAG CTGGTCTTGG CTCTCTATGA CTATCAAGAG 
AAGAGCCCTC GTGAGGTCAC CATGAAGAAA GGGGATATCC TCACCTTGCT CAACAGCACA 
AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGTCAGG GTTTTGTGCC AGCTGCGTAT 
GTGAAGAAGC TGGACCCCGC CCAGTCAGCC TCAAGGGAGA ACCTCCTGGA AGAACAGGGC 
AGCATTGCTC TGCGGCAGGG GCAGATCGAC AACCAGTATC AGTCTCTGCT GGAGCTGGGT 
GAGAAGAGAA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TCATGTTGTT CCGGGAAGCG 
AACGAGCTAC AGCAGTGGAT CAACGAGAAG GAAGCTGCTC TAACGAGTGA AGAGGTTGGC 
GCTGACTTGG AGCAGGTCGA GGTGCTGCAG AAGAAGTTCG ATGACTTCCA GAAGGATCTG 
AAAGCCAATG AGTCCCGGCT GAAGGACATT AACAAAGTGG CCGAGGACCT GGAGTCTGAA 
GGTCTCATGG CGGAAGAAGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG TATGATGCCC 
AGGGATGAAG CAGATTCCAA GACCGCCTCC CCATGGAAGT CTGCTCGACT GATGGTCCAC 
ACAGTGGCCA CCTTCAACTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTGCAACAG 
CTGGCTGAGG AACGTAGCCA GCTCCTGGGC AGTGCACACG AAGTACAGAG GTTCCACAGG 
GATGCGGATG AAACCAAAGA ATGGATTGAG GAGAAGAACC AGGCTCTGAA CACAGACAAC 
TATGGCCATG ATCTAGCTAG CGTCCAGGCC CTGCAGCGCA AACACGAAGG CTTCGAGAGG 
GACCTTGCAG CTCTTGGTGA CAAGGTGAAT TCCCTTGGGG AAACAGCCCA GAGGCTGATC 
CAGTCCCACC CTGAATCTGC AGAGGACTTA AAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGACCAGCC TAGGGAAGCG TGCAGACCAG CGCAAGGCCA AACTGGGTGA CTCCCATGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGAGGG 
TTGGTATCTT CAGATGAACT GGCCAAGGAT GTCACTGGAG CTGAGGCTTT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATTGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TATGCCAGCC CAGAGATCAA GGAGAAACTT 
GATATTCTTG ACCAGGAGCG CACAGACCTG GAGAAGGCCT GGGTTCAGCG CAGAATGATG 
CTGGACCACT GCCTGGAGTT GCAGCTGTTC CATCGAGACT GTGAGCAAGC AGAGAACTGG 
ATGGCTGCCC GGGAAGCCTT CCTAAACACA GAAGACAAAG GAGACTCGCT GGACAGTGTG 
GAGGCTCTGA TCAAAAAACA TGAAGACTTC GACAAAGCTA TCAATGTCCA GGAGGAGAAG 
ATAGCTGCCC TGCAGGCCTT TGCCGACCAG CTCATTGCTG TGGACCACTA TGCCAAGGGA 
GACATTGCAA ACCGACGCAA TGAGGTCCTG GACAGGTGGC GCCGCCTAAA AGCCCAGATG 
ATTGAGAAAA GGTCAAAGCT CGGAGAATCT CAAACACTTC AGCAGTTCAG CCGGGATGTA 
GATGAGATTG AAGCCTGGAT CAGTGAGAAG TTACAAACAG CCAGCGATGA GTCATACAAG 
GACCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ACCAGAAGCA CCAAGCCTTT 
GAGGCAGAAC TGCACGCCAA TGCTGACCGA ATCCGTGGAG TTATTGACAT GGGCAACTCC 
CTCATTGAGC GTGGGGCCTG TGCTGGCAGT GAGGATGCTG TCAAGGCCCG CCTGGCTGCC 
CTTGCAGACC AGTGGCAGTT CCTGGTGCAG AAGTCAGCTG AGAAGAGCCA GAAGCTGAAA 
GAGGCCAATA AGCAGCAGAA CTTCAACACC GGGATCAAAG ACTTTGACTT CTGGCTTTCT 
GAGGTGGAGG CTCTCCTGGC ATCTGAAGAC TACGGCAAAG ACCTGGCTTC CGTGAACAAC 
CTGCTCAAAA AGCATCAGCT GCTGGAGGCA GACATATCGG CCCACGAGGA TCGTCTGAAG 
GACCTGAACA GCCAGGCTGA CAGCCTGATG ACTAGCAGTG CCTTCGACAC CTCCCAAGTG 
AAAGAGAAGC GGGACACCAT CAATGGACGC TTTCAGAAGA TCAAGAGCAT GGCAACCTCC 
CGAAGAGCAA AACTGAGCGA GTCCCATCGC CTGCACCAGT TTTTCCGAGA CATGGATGAC 
GAGGAGTCCT GGATCAAGGA GAAGAAGTTG TTAGTGAGCT CTGAGGACTA TGGCAGAGAC 
CTCACTGGTG TTCAAAATCT GAGGAAGAAA CACAAGCGGC TAGAAGCCGA ACTGGCCGCA 
CACGAACCAG CCATTCAGGG TGTCCTGGAC ACGGGGAAGA AGCTGTCTGA TGACAACACC 
ATCGGGCAGG AGGAGATCCA GCAGCGTCTC GCACAGTTTG TGGAGCACTG GAAGGAACTG 
AAACAGCTAG CAGCTGCACG GGGCCAGCGG CTGGAGGAGT CCTTGGAGTA TCAGCAGTTT 
GTGGCCAACG TGGAGGAGGA GGAGGCTTGG ATCAATGAGA AGATGACCCT GGTGGCCAGC 
GAAGACTACG GGGACACTCT TGCTGCCATC CAGGGCTTAC TGAAGAAACA TGAAGCTTTT 
GAGACAGACT TCACTGTCCA CAAGGATCGA GTGAATGATG TCTGTACTAA TGGACAAGAC 
CTCATTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CAAAGATGAA GGGTCTGAAT 
GGTAAAGTGT CTGACCTGGA GAAAGCAGCA GCTCAGAGGA AAGCGAAGCT GGATGAGAAC 
TCGGCCTTCC TTCAGTTCAA TTGGAAGGCT GACGTGGTGG AGTCCTGGAT TGGTGAAAAG 
GAGAACAGCT TGAAAACAGA TGATTATGGC CGAGATCTGT CTTCTGTCCA AACTCTGCTC 
ACCAAGCAGG AGACATTTGA TGCTGGCCTG CAGGCCTTCC AGCAGGAGGG CATTGCCAAT 
ATCACTGCCC TCAAAGACCA GCTGCTAGCT GCCAAGCACA TTCAGTCGAA GGCCATCGAG 
GCCCGACATG CCTCCCTCAT GAAGAGGTGG ACCCAGCTGT TGGCCAATTC AGCTACCCGC 
AAGAAGAAGT TGCTAGAGGC CCAGAGTCAT TTCCGAAAGG TAGAAGACCT CTTCCTGACC 
TTTGCCAAAA AGGCATCGGC TTTCAACAGC TGGTTTGAGA ATGCAGAAGA GGACCTCACA 
GACCCAGTGC GCTGCAACTC TCTGGAAGAA ATCAAAGCCC TCCGAGAGGC TCATGATGCC 
TTCCGCTCAT CGCTCAGCTC TGCGCAGGCC GACTTCAACC AGCTAGCCGA GCTGGACCGT 
CAGATCAAGA GTTTCCGAGT GGCCTCCAAT CCCTACACCT GGTTCACCAT GGAGGCCCTG 
GAAGAGACGT GGAGGAACCT ACAGAAGATC ATTAAGGAGC GAGAACTGGA GCTGCAGAAG 
GAACAGCGGC GGCAGGAGGA GAATGACAAG CTACGCCAAG AGTTTGCCCA GCATGCCAAC 
GCGTTCCACC AGTGGATCCA GGAAACAAGA ACGTATCTCC TCGACGGGTC CTGCATGGTC 
GAAGAGTCGG GAACTCTGGA ATCTCAGCTT GAAGCTACCA AACGCAAGCA CCAGGAGATT 
CGGGCCATGA GAAGTCAGCT GAAGAAGATT GAGGACCTGG GGGCTGCCAT GGAGGAAGCC 
CTCATCCTGG ACAACAAGTA CACTGAGCAC AGCACTGTGG GCCTGGCCCA GCAGTGGGAC 
CAGTTAGACC AGCTGGGCAT GCGCATGCAG CACAACCTGG AGCAGCAGAT CCAGGCCAGG 
AACACAACAG GAGTCACTGA GGAGGCCCTC AAGGAGTTCA GCATGATGTT CAAACACTTC 
GACAAGGACA AGTCTGGCCG GCTGAATCAT CAAGAGTTCA AATCCTGCCT TCGTTCTCTG 
GGTTACGACC TGCCAATGGT TGAGGAAGGA GAGCCTGATC CTGAGTTTGA GGCCATACTG 
GACACTGTTG ATCCCAACAG GGACGGCCAC GTCTCCCTGC AAGAGTACAT GGCTTTCATG 
ATCAGCCGTG AAACCGAGAA TGTCAAGTCC AGTGAAGAGA TCGAGAGTGC TTTCCGGGCC 
CTCAGCTCCG AGGGCAAGCC TTATGTGACC AAGGAGGAGC TCTACCAGAA CCTGACCCGG 
GAACAAGCTG ACTACTGTGT CTCCCACATG AAGCCCTATG TGGATGGCAA GGGCCGCGAA 
CTTCCAACTG CCTTCGACTA CGTGGAGTTC ACCCGCTCTC TCTTTGTGAA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Critical roles of ?II spectrin in brain development and epileptic encephalopathy.
The Journal of clinical investigation(2018 Jan)
Wang Y,Ji T,Nelson AD,Glanowska K,Murphy GG,Jenkins PM,Parent JM

E-cadherin interactome complexity and robustness resolved by quantitative proteomics.
Science signaling7(354)rs7(2014 Dec)
Guo Z,Neilson LJ,Zhong H,Murray PS,Zanivan S,Zaidel-Bar R

Proteomic analysis of cerebrospinal fluid extracellular vesicles: a comprehensive dataset.
Journal of proteomics106191-204(2014 Jun)
Chiasserini D,van Weering JR,Piersma SR,Pham TV,Malekzadeh A,Teunissen CE,de Wit H,Jim?nez CR

The giant spectrin ?V couples the molecular motors to phototransduction and Usher syndrome type I proteins along their trafficking route.
Human molecular genetics22(18)3773-88(2013 Sep)
Papal S,Cortese M,Legendre K,Sorusch N,Dragavon J,Sahly I,Shorte S,Wolfrum U,Petit C,El-Amraoui A

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Sptan1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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