Piezo cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following Piezo gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Piezo cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa14091 NM_135344.6
Latest version!
Drosophila melanogaster piezo, transcript variant J (Piezo), mRNA. pcDNA3.1-C-(k)DYK or customized vector 22-24 $1371.30
$1959.00
OFa15938 NM_001273298.1
Latest version!
Drosophila melanogaster piezo, transcript variant L (Piezo), mRNA. pcDNA3.1-C-(k)DYK or customized vector 22-24 $1371.30
$1959.00
OFa15939 NM_001201789.2
Latest version!
Drosophila melanogaster piezo, transcript variant H (Piezo), mRNA. pcDNA3.1-C-(k)DYK or customized vector 22-24 $1371.30
$1959.00
OFa15942 NM_001201790.2
Latest version!
Drosophila melanogaster piezo, transcript variant I (Piezo), mRNA. pcDNA3.1-C-(k)DYK or customized vector 22-24 $1371.30
$1959.00
OFa15937 NM_001273297.1
Latest version!
Drosophila melanogaster piezo, transcript variant K (Piezo), mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OFa15941 NM_001273299.1
Latest version!
Drosophila melanogaster piezo, transcript variant M (Piezo), mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OFa14089 NM_164795.6
Latest version!
Drosophila melanogaster piezo, transcript variant E (Piezo), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OFa14090 NM_001042881.5
Latest version!
Drosophila melanogaster piezo, transcript variant F (Piezo), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OFa15940 NM_001169436.3
Latest version!
Drosophila melanogaster piezo, transcript variant G (Piezo), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OFa41330 NM_001273300.2
Latest version!
Drosophila melanogaster piezo, transcript variant O (Piezo), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OFa41352 NM_001316385.1
Latest version!
Drosophila melanogaster piezo, transcript variant P (Piezo), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa14091
    Clone ID Related Accession (Same CDS sequence) NM_135344.6
    Accession Version NM_135344.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7866bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product piezo, isoform J
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_135344.5. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    ATGGTCTTCA GCTATGCGTG CATGGTGCTC CAGCGCATCG TGGTGCCAGC GGTTCTGGTA 
    CTCGCTGCCC TGATGCGACC AGTGGGCATA TCCTTTGTCT ACCTTTTGAT GTTCTTTGTG
    TCGCCCTTTG TTCCGCTGGC CACGCGACGC AACTTCAAAG GATCTGTGAC TGCCTTCTTC
    ATCATCCTGC TGACGTTGAG CACGCTGGTC CTCTTGGGTC ACATAACGCT ACAGATTCTG
    GCGGTCAGCC TCACGCTGCC CATCTACAAT TGCTCGTTCA GTGAGCGTCT GCTGCGTCAC
    ATTGGCTTCG TGAGCTTTAT TGATCTACAG CCATTTGCCA TCATCGAATG GCTAGTGCCT
    GAGGTGTTGG TTTTCGCCAC CTCCCTGGGT TCGTATCTCA CGGTGAAGCG AGTGGCCTCA
    CAGCCCGTCG GCGCCGAGCA GCTGGAGAAC GGCGAGGTGG TCGATGGCCA GGCGGAAAAC
    GCGCAGACAT CTTCTCAGCC ATCTGCCGCA GATGCCAATG GAGGAGATGT GCAACAGGCC
    ACGGTCACCA CGCCGCTGCA GCAACAGCAG CAGCAGCTGA GGAAACGAGT GTCCATGATC
    AGCCAGCACA TACACTTTGA GGGCTTGGTC AAGATCTCTC CTCTGTTCTG CCTGGCCACG
    CTGTTCTTTG CGGCCGTACT GCGTCCCTCG GTGCCTGGCG GATTTTACTT TCTCATTTTC
    CTGCTGTCCG GCACCTACTG GGCAACATGC CAGACGCTGC AACGGGGCTT CGCTTTGTTG
    CTGCGCTGCG TGATGGTCGT CCTCGTGCTG CACTCCCTGT CCATTGTGTC ATACCAGACG
    CCATGGATGC AGAGCCACCT CAATCATACC ACCCTGACAG CTCGATTGAT TGGACTGGAA
    CCGCTTATTG AATCCTACTG CTCGCCGGAT ATACGAGTCT TTCTGTACAA TAATAAGCTA
    TCCCTGGACT CGTACCTCAA TCCCTTTGCG TTGTTCTTTG CCTATTTCGC ACTGGCACTG
    ACCACCAAGC ATCTGATTAA GCCACGGGTT TCTTTGCGCC GAGATCAGCG TGCAACGTTA
    AATGAACCGA CTGAGACGAC GCCTTTGGTG CGGCAAAGCA CGCGAAAGGC ACGTACACCC
    CAACCGCTGG AAAGTGGATC CTCGGTGGCG CCAAGTGTCA CTCAGCGGGG CAACGACATG
    CAGCTGGAAT CGATGGAGCA GCGATCGGAA CAGGAGAACA CCACCACATC CATACTGGAT
    CAAATATCGT ATGGATTCGT CAGCGTGGGA GGATTCATAT ATCAGAATAG CTATATATTC
    ACCAATATTC TTATGATGGC CTGGTCCATA GTGTATCACA GTTGGCTGAC TTTTGTCCTC
    TTGCTGTCGG CCAACGTACT GTGGATGATT CCCAACCAGA GGAAGGCCAT GATGCGGTCC
    AGTCCCTTTA TAGTTCTGTA TGCTGAGGCC CTTTTGATTG CCCAATACAT ATACGGCATG
    GATCTCAACA ACGAAGAGCT GCCCACGAGC GTTCCCACTG CGGGCATTAA CCTGCAACAA
    ATTGGCTTCG AACGACCCAT CGAGAACCAA ATGCGGCCAT GTGTGCCGCT GATCGTGAAG
    ACAGCGTTTG TGCTAATGTT TTGGGTGACA TCACGACAGT TCTTCAAGGA GAAGCGCGAT
    CGCCGACGGG ACAGCACTCT GGCGGACTTC ATTGCCCCAC TGCAGATTAC TGTGGGATCG
    GCTGGCTCCA GCTACCTCAT CAACGATGGC AAGAAGACCT CAAAGTTCCT AAAGAAGGCC
    GGCGATGTGA TCAAAAATCT ATTGGTGCGT CTGTGGATCT GGCTACTCGT GCTGGTTATC
    TTCCTTTGCG CCATCACTGG CGAGAACATG ACCGGCTTCC GCATCTGCTA CATGGCCCTG
    TTTCTATTCT TCTTGCTAGT CTTTCAATCT TCGTCCAAGG CGTGGGTTAA GATCATGTAC
    GGCTTCTGGC TGTTTCTGAT CTTCTATGCC ATGTCCATAC TTATATTGAT CTACACATAT
    CAATTCGACA AGTTCGACAC GTACTGGAGC GACTATCTTA ATGTGTCCGC GACTTTGCAA
    AAGGACATCG GCCTTAAGCG CTATCAGACC AAGGATCTGT TCCTCCATTT GGTCTCACCG
    ACGATAATTG TGATCCTGAC CGTCATACAA GTGCACTACT TCCACAAGCG CTTTATTGCA
    TCATTGCAGC AGCAGCCGTT GGCTGGCGGA TCGGCACAGC AGAAACCCAC GGAGACAACT
    GCCTTGGAAC CGGCGCCATC GAAGCGACGT GGCAGCGCCG GTTCACTGCG TAAATCTCAG
    GGTCCATCGG CGGAGGCTGC TCCAGGAGCC ACCACCGATT TCGAGACATC TGTGCGAGAC
    TTGGTGCGCA TATCGTTCCG CAAGATCAAG AACAAGTCGG AGTACATCTT CAAGAACTTC
    AAGGATGTCT TCTGGCGCTT CCTGGAGCTG CACATCATGA AGGCTGTGTA TATCGCAGCC
    TTCGTGTGCA GTGTCAGCGA AGTCTGCGTA CTGCACATTA TCTTTGTGGG TTTCTGTGTG
    CTGGGCGCCA CCTCGCGGAA GGCCGTTCAG GTGGTGATCA GCCGCCTCAT CTCGTTCATT
    GTCACCGTCA TAGTTCTGTC CAAGATGATC TACCAGATCG AGTACTTGAG TCACTCGCAG
    CACAATGTGG TTTGTTCTGA CAACCGGACT GCCAACAATG CGGAGTGGAT TGGTCTCACC
    AAGGCTGACA AGGTAACTGG CGGACTGATG AGCCTGTTGC GCACCTACAT CATCTACATG
    GTTATTGTGA CCATGCACGC AGTGATCAGT TTGCGGCAGC TTCAAATGCG CGTCAAGATC
    GGAGCACTGA ATGCTCCACC CACCAAGCTG CTGTTCCCCA ATATTATTCG AGCTGATGCT
    GAGAAGGATC TGGTGGGACT GGTCAAGTAT CTCCTCAACT TTGGCTTCTA CAAATTCGGC
    ATTGAGATAT CGCTAATCGC GCTGGTCTCT ACCATCACAT ATCGCCAGGA TATTGTTGCC
    GTAGTCTATG CTCTGTGGCT AGTGGTGCTT TTGCTTCTAC GGAGATCGCA GTGCGCCAAA
    ATATGGGGCG TGTTTCAGGC ATTCTTTGCC ATCTCCATAC TGACACAGTA TATAGTGCTG
    GTAGGATTGC CGCCGAGCTC ATGCCTGGTG TTTCCCTGGG ATGAAGGTCC CTTCGGCGAG
    GGCATACAAC GCTGGGCGAT GCTGCCAGGA GCCCTGCACT TCAACCACGT ACCCAAGCTG
    ATCTTCGACT TTATTGTCTT GGTCATTCTG AATCGACAGA AGAGTATCTT CTGCATCGAA
    CAGCGTTATG CCAGTAACGA TGACTACCCG GGTGGCAGCA ATCGCAGTGT GATCGCGGAT
    ATTGCTCAGC TAGGTCGCGT TCCCTTCGAC AATCCCACCC ACGACTTTTG TTCGTACATA
    CGCAACTACT CGGACATCCT TAAGAACGGA GTGCTGTGCG GCTTCTACTG GTTTACTCTG
    GCAGTTGTTT TCTTGGCCGG CACCAATATT GCTGATCTGC TGGCACTGGG TTATCTGATC
    GGAGCGTTTA TCTTCCTGTG GCAGGGATCG GATTTCTATC TGCGTCCCAT ACACACCATC
    ATCTTTCGCT GGAAGTGGCT GCTGGCATTC AATGTGGCCA ACATACTTAT CAAGACGTCC
    TTCCAAATGG CCGGCTGTTT GTTCATGACC CAACTGACGA AAGACTGCTG CTGGCTGGTG
    CACATGCTCG GCATCACATG TACGAGCAAT GTACTTACAG AGCAAATAAT GCTGCCCGAG
    GAGGCTGAAT TGGCGCTTAA GCCAGGCGAA TGTCCTAAGA TCACCCACCA GGTGGTCCTT
    CTGTGGGACA CGATTTGCTT TGCCTTCATC ATCTTCCAGC TGCGCATCTT CAAGTCGCAC
    TACTTCTGTC ACATCATAAC GGACACCAAA GCAAATAACA TCCTGGCCTC AAGAGGAGCC
    GACATCATTG AGAGCCTACG ACATAAGCAG ATTGCACATC GACACGACCA TGAAAAGCAG
    GTGCTGCATA AGATCAAGCG AAAGATGGAG CGCATCCGTG CCACGCAGCA GAAGATGCTA
    CGACCCTTGG ACAAACAAAC CCACTTCGAC GAACATGGTT ATCCACTTCC TGCACCAACA
    GTACGCAGAA GGAAGGAAAT TAAATTACAT CCACATGCTA CCCGTGCTGG TGACTACTAT
    ATGTTCGAGG AGATGGACGA TAAGTTTGAG CTCGACTTGA TACACGACGA GATCGACTTC
    CTCGAGGAGG AGAACATCAC CGAGAGCGAG ATGAAGATGC AGCGACGCAA GACCCTCTAC
    GATAAGTCGA AGGATGCACC CACAGGCGAG TTTCCCTCCA CCAGTAAGGG TATTTCGAAG
    GAGCGCGATG CGGCAACGGC TTCCAGTTCG GCTAGTCCAG CGCCCACTAG GGATGTGGGT
    GATCTGCCTG TGATTCCACC ACCCTCCACT GGCCTGGGAC GTGAGCAAAC CTCCAAGGAG
    ACCTCCGATA GCAAGTCCAA AATGGAAGTG GACAGCGGAG AGGTGACGGC CAAGGATTCG
    GATGAGGACT TCGATACCAA TCCCATTATC AGACTGCTCG AGGGCTTCTT GGTCACGCTG
    ACCATAAGAC TGAACCGCTT TTCGCGCAAC TATCGCTTTG TGAATCGCAT CCTGGCCGGC
    GAAAAGAAGA CCCTAAAGGA GTCCAGTTCG TTGAACCGTC TGGGGCTGTC CAGTGCCGCT
    GCCATGTTCC ACTTCCTCAA ATCGAATTTA GAGAGCGATG AAAGTGAACC GCCCGCCTCC
    TCATCCACCC CGCGGCGGGT GGTGATCGCA CCACCGAATG CCACCGAGCA CTCAGACCCC
    ACCAGCACCA CACTGAACAC GAACACGACA ACCACACCGC TATCACCACC AGAACCACTG
    CAACCACTGC AACCACTGCA ACCAAATACA ACCAGTACAC CACAGCAACA GCATCAGCAT
    ATTCGCGCTG CCGAAGAAAT CATCGAACTG CCTGTAGATA CCGTGGATGG AGTCGCCCAT
    AGAAAACAAT CAATCAATTC ATCGCCGCCA GCCAAGGGCA CGATGCTCAG TCGAAAATCG
    GATTGTGGCC TGCCTGAGAT CCGTATTAAA GCGCCATCTA TCGAGCGCGG TGCACACTAT
    TACCATAATC ACCACAGCGG CGGTGGCTCA GGATCCTTGA GCAAACACTG GTCCTACGAG
    CAGGTGGACA GCGCGGGTGA ATTCAATCTG GAGGAGGAGA ACTTCGCCCA GAGAGATCAT
    CACATCATTG TCGAGGTGTT GATCTCCTCG TGGTATGCCC TGTTGGCCAA CACGGATCTC
    ATCTGCTACA TTGTGGTTTT CATCAATCAG GTGGTCAATG CCAGTCTCAT TTCGCTGCCG
    CTGCCCATTA TGGTCTTTTT GTGGGGCACA TTGTCTCTGC CGCGTCCCAC TAAAACCTTC
    TGGGTCACGC TGATTGCCTA CACCCAGGCC ATCGTGCTGA TCAAGTGCAT CTTCCAGTTT
    AAGCTGATCT GGTCCAATTA CCACCAACTG CCCAATCAGC CGCTAACACC GGCCAAAATA
    TTCGGCGTTG AGAATAAGGC CCATTATGCG ATTTACGACC TGATACTATT GCTGGTTCTA
    TTCCTGCATC GCTATTTGCT TAAATCACAA GGCCTGTGGA AATCGGGCTA CAAGGACACG
    GATAACCAGT TCACCAAGCC CACCGCTAGC ATTGATGAAC GCGACGATAG CGACAACTTA
    TCCCAACCGG ATTCCCGCCA GCTAAACGAT GATGCTGCTC AGAAGCTGAG TCTCCAAGTG
    AGCCAGGCTT CCTTGCCAGG ATCGCCCGAG TTTAGCAAGA CAGGCATCAA TCAGCTAGAG
    CGCACCAAGT ACACTTCATC GCTGTACAAA TTCTTCTTCA GTTTGGTCCA CAAATCCCGT
    CTAGCCACAG ATGTTTACGC TCTGATGTTC CTCTGCGATT TTGTGAACTT CTTCGTGCTT
    CTTTTCGGCT TCACTGCTTT TGGAACTCAG CAAACGGAAA GTGATGAAGG TGTGCAGACA
    TATCTTGCGG AGAACAAAGT GCCCATACCA TTCCTGATTA TGCTACTTGT TCAGTTCCTG
    CTCATTGTCA TTGATCGAGC TTTGTATCTG CGCAAAGCCC TGGTGAACAA GATCATCTTC
    CACTTCTTTT CGGTTATCGG AATACACATC TGGATGTTCT TCGTTGTGCC TGCAGTTACG
    GAGCGCACTT TCAACTCCCT CGCACCTCCA ATAATATTCT ACGTTATCAA GTGCTTTTAC
    ATGCTGCTCA GCTCTTATCA AATCAAATCC GGTTACCCTA AGCGCATTCT AGGCAACTTC
    TTCACCAAGG GTTTCTCGAT GGTCAATATG ATTGCCTTTA AGGTGTACAT GCAGATTCCA
    TTCCTGTACG AGCTGCGAAC CATATTAGAT TGGGTGTGCA TTGACAGCAC CATGACCATT
    TTCGATTGGC TAAAGATGGA GGACATTTTC TCGAATATAT ATCTTATCCG CTGCACCCGG
    CAGTCAGAGA CTGATTTTCC GGCCATGAGA GCTCAGAAGA AGGCCTCACT TTCAAAGCTG
    ATAATGGGTG GCACCATCGT CCTGCTGATA GTGATTTGCA TTTGGGGACC ATTGTGTTTG
    TTTGCACTTG GCAACGCGGT GGGCACCTCG AATGTGCCGT TCCATGTGTC GCTATCAATA
    CGAATTGGAC CCTATGATCC CATCTACACA ACGAACAACT ACGACAGTAT CTTCGAGATC
    AATCCTGAGA TGTACTCTCA GATGACTAAT GCATACATAA AGGAGAAACA GGCTTTGACG
    TTTATTGCCG GCTATGATGC AACGGATGTA GCGGCGGTTA GACTAGCTGG CAACTCGCCA
    TCTCTGTGGA ACATAGCACC GCCGGATAGG CAGCGACTAC TAAATGATTT AAGAAACAAT
    CACACGCTGA AGGCCCGGTT CTCTTATTCC CTCACCCGAA AGGCTCCTGC CAAGGGGCTA
    AAGGAAAATG TGGGCGACGA GCATGCCATA TCGCTGGACG AGTCCTTTGA GGGACGAGCA
    GCACTCATTC ACATGCTAAG CGAAACCCAT GATGTGGAGC CCATTTACAG CAATGGCACC
    ACAAATGGAA CCACTCCCGA AGTTGAAGAA GTGGTGGTGA TACCCGGAAT GATACCCAAG
    TTTATCAAGG TTCTCAATTC GGGCGACGCT GCGGTGGTAA GTGTTTTGAG CCCAAAACAC
    TACGACTACC GACCGTTGGT TATCAAAATG CATCGGGACA ACGAGACCAA TGGGTTGTGG
    TGGGAGATTC GGGACTATTG CAACGACACC TTCTACAACG AAACTCTATC GAAGTTTGCA
    TACAGCAACT GCACTTCGGG AATTGTGATG TACACGTTCA ACGATAAAAA GTTTCCATCC
    ACGTTCAGTT TCCTCACAGC GGGAGGCATC ATTGGTCTGT ACACTACCTT TGTGTTATTG
    GCCTCGCGCT TTATGAAGTC CTTCATTGGC GGGCAAAACA GAAAGATCAT GTTTGAGGAT
    CTGCCCTATG TTGATAGAGT GCTGCAGCTC TGTCTGGACA TTTATCTGGT ACGGGAGGCA
    TTGGAATTCG CTTTGGAAGA GGACCTGTTT GCCAAATTAC TCTTCCTATA CCGATCGCCC
    GAAACGCTAA TCAAGTGGAC GCGTCCCAAG GAGGAGTACG TGGACGATGA TGGCGACACC
    GACTCGATTC CCAGTCGAAT GAGCGTGCGC CGGCCGGAGC AACTGCAGCC ACAGCAACCG
    CAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_609188.4
    CDS516..8381
    Translation

    Target ORF information:

    RefSeq Version NM_135344.6
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster piezo, transcript variant J (Piezo), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_135344.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    ATGGTCTTCA GCTATGCGTG CATGGTGCTC CAGCGCATCG TGGTGCCAGC GGTTCTGGTA 
    CTCGCTGCCC TGATGCGACC AGTGGGCATA TCCTTTGTCT ACCTTTTGAT GTTCTTTGTG
    TCGCCCTTTG TTCCGCTGGC CACGCGACGC AACTTCAAAG GATCTGTGAC TGCCTTCTTC
    ATCATCCTGC TGACGTTGAG CACGCTGGTC CTCTTGGGTC ACATAACGCT ACAGATTCTG
    GCGGTCAGCC TCACGCTGCC CATCTACAAT TGCTCGTTCA GTGAGCGTCT GCTGCGTCAC
    ATTGGCTTCG TGAGCTTTAT TGATCTACAG CCATTTGCCA TCATCGAATG GCTAGTGCCT
    GAGGTGTTGG TTTTCGCCAC CTCCCTGGGT TCGTATCTCA CGGTGAAGCG AGTGGCCTCA
    CAGCCCGTCG GCGCCGAGCA GCTGGAGAAC GGCGAGGTGG TCGATGGCCA GGCGGAAAAC
    GCGCAGACAT CTTCTCAGCC ATCTGCCGCA GATGCCAATG GAGGAGATGT GCAACAGGCC
    ACGGTCACCA CGCCGCTGCA GCAACAGCAG CAGCAGCTGA GGAAACGAGT GTCCATGATC
    AGCCAGCACA TACACTTTGA GGGCTTGGTC AAGATCTCTC CTCTGTTCTG CCTGGCCACG
    CTGTTCTTTG CGGCCGTACT GCGTCCCTCG GTGCCTGGCG GATTTTACTT TCTCATTTTC
    CTGCTGTCCG GCACCTACTG GGCAACATGC CAGACGCTGC AACGGGGCTT CGCTTTGTTG
    CTGCGCTGCG TGATGGTCGT CCTCGTGCTG CACTCCCTGT CCATTGTGTC ATACCAGACG
    CCATGGATGC AGAGCCACCT CAATCATACC ACCCTGACAG CTCGATTGAT TGGACTGGAA
    CCGCTTATTG AATCCTACTG CTCGCCGGAT ATACGAGTCT TTCTGTACAA TAATAAGCTA
    TCCCTGGACT CGTACCTCAA TCCCTTTGCG TTGTTCTTTG CCTATTTCGC ACTGGCACTG
    ACCACCAAGC ATCTGATTAA GCCACGGGTT TCTTTGCGCC GAGATCAGCG TGCAACGTTA
    AATGAACCGA CTGAGACGAC GCCTTTGGTG CGGCAAAGCA CGCGAAAGGC ACGTACACCC
    CAACCGCTGG AAAGTGGATC CTCGGTGGCG CCAAGTGTCA CTCAGCGGGG CAACGACATG
    CAGCTGGAAT CGATGGAGCA GCGATCGGAA CAGGAGAACA CCACCACATC CATACTGGAT
    CAAATATCGT ATGGATTCGT CAGCGTGGGA GGATTCATAT ATCAGAATAG CTATATATTC
    ACCAATATTC TTATGATGGC CTGGTCCATA GTGTATCACA GTTGGCTGAC TTTTGTCCTC
    TTGCTGTCGG CCAACGTACT GTGGATGATT CCCAACCAGA GGAAGGCCAT GATGCGGTCC
    AGTCCCTTTA TAGTTCTGTA TGCTGAGGCC CTTTTGATTG CCCAATACAT ATACGGCATG
    GATCTCAACA ACGAAGAGCT GCCCACGAGC GTTCCCACTG CGGGCATTAA CCTGCAACAA
    ATTGGCTTCG AACGACCCAT CGAGAACCAA ATGCGGCCAT GTGTGCCGCT GATCGTGAAG
    ACAGCGTTTG TGCTAATGTT TTGGGTGACA TCACGACAGT TCTTCAAGGA GAAGCGCGAT
    CGCCGACGGG ACAGCACTCT GGCGGACTTC ATTGCCCCAC TGCAGATTAC TGTGGGATCG
    GCTGGCTCCA GCTACCTCAT CAACGATGGC AAGAAGACCT CAAAGTTCCT AAAGAAGGCC
    GGCGATGTGA TCAAAAATCT ATTGGTGCGT CTGTGGATCT GGCTACTCGT GCTGGTTATC
    TTCCTTTGCG CCATCACTGG CGAGAACATG ACCGGCTTCC GCATCTGCTA CATGGCCCTG
    TTTCTATTCT TCTTGCTAGT CTTTCAATCT TCGTCCAAGG CGTGGGTTAA GATCATGTAC
    GGCTTCTGGC TGTTTCTGAT CTTCTATGCC ATGTCCATAC TTATATTGAT CTACACATAT
    CAATTCGACA AGTTCGACAC GTACTGGAGC GACTATCTTA ATGTGTCCGC GACTTTGCAA
    AAGGACATCG GCCTTAAGCG CTATCAGACC AAGGATCTGT TCCTCCATTT GGTCTCACCG
    ACGATAATTG TGATCCTGAC CGTCATACAA GTGCACTACT TCCACAAGCG CTTTATTGCA
    TCATTGCAGC AGCAGCCGTT GGCTGGCGGA TCGGCACAGC AGAAACCCAC GGAGACAACT
    GCCTTGGAAC CGGCGCCATC GAAGCGACGT GGCAGCGCCG GTTCACTGCG TAAATCTCAG
    GGTCCATCGG CGGAGGCTGC TCCAGGAGCC ACCACCGATT TCGAGACATC TGTGCGAGAC
    TTGGTGCGCA TATCGTTCCG CAAGATCAAG AACAAGTCGG AGTACATCTT CAAGAACTTC
    AAGGATGTCT TCTGGCGCTT CCTGGAGCTG CACATCATGA AGGCTGTGTA TATCGCAGCC
    TTCGTGTGCA GTGTCAGCGA AGTCTGCGTA CTGCACATTA TCTTTGTGGG TTTCTGTGTG
    CTGGGCGCCA CCTCGCGGAA GGCCGTTCAG GTGGTGATCA GCCGCCTCAT CTCGTTCATT
    GTCACCGTCA TAGTTCTGTC CAAGATGATC TACCAGATCG AGTACTTGAG TCACTCGCAG
    CACAATGTGG TTTGTTCTGA CAACCGGACT GCCAACAATG CGGAGTGGAT TGGTCTCACC
    AAGGCTGACA AGGTAACTGG CGGACTGATG AGCCTGTTGC GCACCTACAT CATCTACATG
    GTTATTGTGA CCATGCACGC AGTGATCAGT TTGCGGCAGC TTCAAATGCG CGTCAAGATC
    GGAGCACTGA ATGCTCCACC CACCAAGCTG CTGTTCCCCA ATATTATTCG AGCTGATGCT
    GAGAAGGATC TGGTGGGACT GGTCAAGTAT CTCCTCAACT TTGGCTTCTA CAAATTCGGC
    ATTGAGATAT CGCTAATCGC GCTGGTCTCT ACCATCACAT ATCGCCAGGA TATTGTTGCC
    GTAGTCTATG CTCTGTGGCT AGTGGTGCTT TTGCTTCTAC GGAGATCGCA GTGCGCCAAA
    ATATGGGGCG TGTTTCAGGC ATTCTTTGCC ATCTCCATAC TGACACAGTA TATAGTGCTG
    GTAGGATTGC CGCCGAGCTC ATGCCTGGTG TTTCCCTGGG ATGAAGGTCC CTTCGGCGAG
    GGCATACAAC GCTGGGCGAT GCTGCCAGGA GCCCTGCACT TCAACCACGT ACCCAAGCTG
    ATCTTCGACT TTATTGTCTT GGTCATTCTG AATCGACAGA AGAGTATCTT CTGCATCGAA
    CAGCGTTATG CCAGTAACGA TGACTACCCG GGTGGCAGCA ATCGCAGTGT GATCGCGGAT
    ATTGCTCAGC TAGGTCGCGT TCCCTTCGAC AATCCCACCC ACGACTTTTG TTCGTACATA
    CGCAACTACT CGGACATCCT TAAGAACGGA GTGCTGTGCG GCTTCTACTG GTTTACTCTG
    GCAGTTGTTT TCTTGGCCGG CACCAATATT GCTGATCTGC TGGCACTGGG TTATCTGATC
    GGAGCGTTTA TCTTCCTGTG GCAGGGATCG GATTTCTATC TGCGTCCCAT ACACACCATC
    ATCTTTCGCT GGAAGTGGCT GCTGGCATTC AATGTGGCCA ACATACTTAT CAAGACGTCC
    TTCCAAATGG CCGGCTGTTT GTTCATGACC CAACTGACGA AAGACTGCTG CTGGCTGGTG
    CACATGCTCG GCATCACATG TACGAGCAAT GTACTTACAG AGCAAATAAT GCTGCCCGAG
    GAGGCTGAAT TGGCGCTTAA GCCAGGCGAA TGTCCTAAGA TCACCCACCA GGTGGTCCTT
    CTGTGGGACA CGATTTGCTT TGCCTTCATC ATCTTCCAGC TGCGCATCTT CAAGTCGCAC
    TACTTCTGTC ACATCATAAC GGACACCAAA GCAAATAACA TCCTGGCCTC AAGAGGAGCC
    GACATCATTG AGAGCCTACG ACATAAGCAG ATTGCACATC GACACGACCA TGAAAAGCAG
    GTGCTGCATA AGATCAAGCG AAAGATGGAG CGCATCCGTG CCACGCAGCA GAAGATGCTA
    CGACCCTTGG ACAAACAAAC CCACTTCGAC GAACATGGTT ATCCACTTCC TGCACCAACA
    GTACGCAGAA GGAAGGAAAT TAAATTACAT CCACATGCTA CCCGTGCTGG TGACTACTAT
    ATGTTCGAGG AGATGGACGA TAAGTTTGAG CTCGACTTGA TACACGACGA GATCGACTTC
    CTCGAGGAGG AGAACATCAC CGAGAGCGAG ATGAAGATGC AGCGACGCAA GACCCTCTAC
    GATAAGTCGA AGGATGCACC CACAGGCGAG TTTCCCTCCA CCAGTAAGGG TATTTCGAAG
    GAGCGCGATG CGGCAACGGC TTCCAGTTCG GCTAGTCCAG CGCCCACTAG GGATGTGGGT
    GATCTGCCTG TGATTCCACC ACCCTCCACT GGCCTGGGAC GTGAGCAAAC CTCCAAGGAG
    ACCTCCGATA GCAAGTCCAA AATGGAAGTG GACAGCGGAG AGGTGACGGC CAAGGATTCG
    GATGAGGACT TCGATACCAA TCCCATTATC AGACTGCTCG AGGGCTTCTT GGTCACGCTG
    ACCATAAGAC TGAACCGCTT TTCGCGCAAC TATCGCTTTG TGAATCGCAT CCTGGCCGGC
    GAAAAGAAGA CCCTAAAGGA GTCCAGTTCG TTGAACCGTC TGGGGCTGTC CAGTGCCGCT
    GCCATGTTCC ACTTCCTCAA ATCGAATTTA GAGAGCGATG AAAGTGAACC GCCCGCCTCC
    TCATCCACCC CGCGGCGGGT GGTGATCGCA CCACCGAATG CCACCGAGCA CTCAGACCCC
    ACCAGCACCA CACTGAACAC GAACACGACA ACCACACCGC TATCACCACC AGAACCACTG
    CAACCACTGC AACCACTGCA ACCAAATACA ACCAGTACAC CACAGCAACA GCATCAGCAT
    ATTCGCGCTG CCGAAGAAAT CATCGAACTG CCTGTAGATA CCGTGGATGG AGTCGCCCAT
    AGAAAACAAT CAATCAATTC ATCGCCGCCA GCCAAGGGCA CGATGCTCAG TCGAAAATCG
    GATTGTGGCC TGCCTGAGAT CCGTATTAAA GCGCCATCTA TCGAGCGCGG TGCACACTAT
    TACCATAATC ACCACAGCGG CGGTGGCTCA GGATCCTTGA GCAAACACTG GTCCTACGAG
    CAGGTGGACA GCGCGGGTGA ATTCAATCTG GAGGAGGAGA ACTTCGCCCA GAGAGATCAT
    CACATCATTG TCGAGGTGTT GATCTCCTCG TGGTATGCCC TGTTGGCCAA CACGGATCTC
    ATCTGCTACA TTGTGGTTTT CATCAATCAG GTGGTCAATG CCAGTCTCAT TTCGCTGCCG
    CTGCCCATTA TGGTCTTTTT GTGGGGCACA TTGTCTCTGC CGCGTCCCAC TAAAACCTTC
    TGGGTCACGC TGATTGCCTA CACCCAGGCC ATCGTGCTGA TCAAGTGCAT CTTCCAGTTT
    AAGCTGATCT GGTCCAATTA CCACCAACTG CCCAATCAGC CGCTAACACC GGCCAAAATA
    TTCGGCGTTG AGAATAAGGC CCATTATGCG ATTTACGACC TGATACTATT GCTGGTTCTA
    TTCCTGCATC GCTATTTGCT TAAATCACAA GGCCTGTGGA AATCGGGCTA CAAGGACACG
    GATAACCAGT TCACCAAGCC CACCGCTAGC ATTGATGAAC GCGACGATAG CGACAACTTA
    TCCCAACCGG ATTCCCGCCA GCTAAACGAT GATGCTGCTC AGAAGCTGAG TCTCCAAGTG
    AGCCAGGCTT CCTTGCCAGG ATCGCCCGAG TTTAGCAAGA CAGGCATCAA TCAGCTAGAG
    CGCACCAAGT ACACTTCATC GCTGTACAAA TTCTTCTTCA GTTTGGTCCA CAAATCCCGT
    CTAGCCACAG ATGTTTACGC TCTGATGTTC CTCTGCGATT TTGTGAACTT CTTCGTGCTT
    CTTTTCGGCT TCACTGCTTT TGGAACTCAG CAAACGGAAA GTGATGAAGG TGTGCAGACA
    TATCTTGCGG AGAACAAAGT GCCCATACCA TTCCTGATTA TGCTACTTGT TCAGTTCCTG
    CTCATTGTCA TTGATCGAGC TTTGTATCTG CGCAAAGCCC TGGTGAACAA GATCATCTTC
    CACTTCTTTT CGGTTATCGG AATACACATC TGGATGTTCT TCGTTGTGCC TGCAGTTACG
    GAGCGCACTT TCAACTCCCT CGCACCTCCA ATAATATTCT ACGTTATCAA GTGCTTTTAC
    ATGCTGCTCA GCTCTTATCA AATCAAATCC GGTTACCCTA AGCGCATTCT AGGCAACTTC
    TTCACCAAGG GTTTCTCGAT GGTCAATATG ATTGCCTTTA AGGTGTACAT GCAGATTCCA
    TTCCTGTACG AGCTGCGAAC CATATTAGAT TGGGTGTGCA TTGACAGCAC CATGACCATT
    TTCGATTGGC TAAAGATGGA GGACATTTTC TCGAATATAT ATCTTATCCG CTGCACCCGG
    CAGTCAGAGA CTGATTTTCC GGCCATGAGA GCTCAGAAGA AGGCCTCACT TTCAAAGCTG
    ATAATGGGTG GCACCATCGT CCTGCTGATA GTGATTTGCA TTTGGGGACC ATTGTGTTTG
    TTTGCACTTG GCAACGCGGT GGGCACCTCG AATGTGCCGT TCCATGTGTC GCTATCAATA
    CGAATTGGAC CCTATGATCC CATCTACACA ACGAACAACT ACGACAGTAT CTTCGAGATC
    AATCCTGAGA TGTACTCTCA GATGACTAAT GCATACATAA AGGAGAAACA GGCTTTGACG
    TTTATTGCCG GCTATGATGC AACGGATGTA GCGGCGGTTA GACTAGCTGG CAACTCGCCA
    TCTCTGTGGA ACATAGCACC GCCGGATAGG CAGCGACTAC TAAATGATTT AAGAAACAAT
    CACACGCTGA AGGCCCGGTT CTCTTATTCC CTCACCCGAA AGGCTCCTGC CAAGGGGCTA
    AAGGAAAATG TGGGCGACGA GCATGCCATA TCGCTGGACG AGTCCTTTGA GGGACGAGCA
    GCACTCATTC ACATGCTAAG CGAAACCCAT GATGTGGAGC CCATTTACAG CAATGGCACC
    ACAAATGGAA CCACTCCCGA AGTTGAAGAA GTGGTGGTGA TACCCGGAAT GATACCCAAG
    TTTATCAAGG TTCTCAATTC GGGCGACGCT GCGGTGGTAA GTGTTTTGAG CCCAAAACAC
    TACGACTACC GACCGTTGGT TATCAAAATG CATCGGGACA ACGAGACCAA TGGGTTGTGG
    TGGGAGATTC GGGACTATTG CAACGACACC TTCTACAACG AAACTCTATC GAAGTTTGCA
    TACAGCAACT GCACTTCGGG AATTGTGATG TACACGTTCA ACGATAAAAA GTTTCCATCC
    ACGTTCAGTT TCCTCACAGC GGGAGGCATC ATTGGTCTGT ACACTACCTT TGTGTTATTG
    GCCTCGCGCT TTATGAAGTC CTTCATTGGC GGGCAAAACA GAAAGATCAT GTTTGAGGAT
    CTGCCCTATG TTGATAGAGT GCTGCAGCTC TGTCTGGACA TTTATCTGGT ACGGGAGGCA
    TTGGAATTCG CTTTGGAAGA GGACCTGTTT GCCAAATTAC TCTTCCTATA CCGATCGCCC
    GAAACGCTAA TCAAGTGGAC GCGTCCCAAG GAGGAGTACG TGGACGATGA TGGCGACACC
    GACTCGATTC CCAGTCGAAT GAGCGTGCGC CGGCCGGAGC AACTGCAGCC ACAGCAACCG
    CAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa15938
    Clone ID Related Accession (Same CDS sequence) NM_001273298.1
    Accession Version NM_001273298.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7656bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product piezo, isoform L
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGTCTTCA GCTATGCGTG CATGGTGCTC CAGCGCATCG TGGTGCCAGC GGTTCTGGTA 
    CTCGCTGCCC TGATGCGACC AGTGGGCATA TCCTTTGTCT ACCTTTTGAT GTTCTTTGTG
    TCGCCCTTTG TTCCGCTGGC CACGCGACGC AACTTCAAAG GATCTGTGAC TGCCTTCTTC
    ATCATCCTGC TGACGTTGAG CACGCTGGTC CTCTTGGGTC ACATAACGCT ACAGATTCTG
    GCGGTCAGCC TCACGCTGCC CATCTACAAT TGCTCGTTCA GTGAGCGTCT GCTGCGTCAC
    ATTGGCTTCG TGAGCTTTAT TGATCTACAG CCATTTGCCA TCATCGAATG GCTAGTGCCT
    GAGGTGTTGG TTTTCGCCAC CTCCCTGGGT TCGTATCTCA CGGTGAAGCG AGTGGCCTCA
    CAGCCCGTCG GCGCCGAGCA GCTGGAGAAC GGCGAGGTGG TCGATGGCCA GGCGGAAAAC
    GCGCAGACAT CTTCTCAGCC ATCTGCCGCA GATGCCAATG GAGGAGATGT GCAACAGGCC
    ACGGTCACCA CGCCGCTGCA GCAACAGCAG CAGCAGCTGA GGAAACGAGT GTCCATGATC
    AGCCAGCACA TACACTTTGA GGGCTTGGTC AAGATCTCTC CTCTGTTCTG CCTGGCCACG
    CTGTTCTTTG CGGCCGTACT GCGTCCCTCG GTGCCTGGCG GATTTTACTT TCTCATTTTC
    CTGCTGTCCG GCACCTACTG GGCAACATGC CAGACGCTGC AACGGGGCTT CGCTTTGTTG
    CTGCGCTGCG TGATGGTCGT CCTCGTGCTG CACTCCCTGT CCATTGTGTC ATACCAGACG
    CCATGGATGC AGAGCCACCT CAATCATACC ACCCTGACAG CTCGATTGAT TGGACTGGAA
    CCGCTTATTG AATCCTACTG CTCGCCGGAT ATACGAGTCT TTCTGTACAA TAATAAGCTA
    TCCCTGGACT CGTACCTCAA TCCCTTTGCG TTGTTCTTTG CCTATTTCGC ACTGGCACTG
    ACCACCAAGC ATCTGATTAA GCCACGGGTT TCTTTGCGCC GAGATCAGCG TGCAACGTTA
    AATGAACCGA CTGAGACGAC GCCTTTGGTG CGGCAAAGCA CGCGAAAGGC ACGTACACCC
    CAACCGCTGG AAAGTGGATC CTCGGTGGCG CCAAGTGTCA CTCAGCGGGG CAACGACATG
    CAGCTGGAAT CGATGGAGCA GCGATCGGAA CAGGAGAACA CCACCACATC CATACTGGAT
    CAAATATCGT ATGGATTCGT CAGCGTGGGA GGATTCATAT ATCAGAATAG CTATATATTC
    ACCAATATTC TTATGATGGC CTGGTCCATA GTGTATCACA GTTGGCTGAC TTTTGTCCTC
    TTGCTGTCGG CCAACGTACT GTGGATGATT CCCAACCAGA GGAAGGCCAT GATGCGGTCC
    AGTCCCTTTA TAGTTCTGTA TGCTGAGGCC CTTTTGATTG CCCAATACAT ATACGGCATG
    GATCTCAACA ACGAAGAGCT GCCCACGAGC GTTCCCTATT TGCAGACTGC GGGCATTAAC
    CTGCAACAAA TTGGCTTCGA ACGACCCATC GAGAACCAAA TGCGGCCATG TGTGCCGCTG
    ATCGTGAAGA CAGCGTTTGT GCTAATGTTT TGGGTGACAT CACGACAGTT CTTCAAGGAG
    AAGCGCGATC GCCGACGGGA CAGCACTCTG GCGGACTTCA TTGCCCCACT GCAGATTACT
    GTGGGATCGG CTGGCTCCAG CTACCTCATC AACGATGGCA AGAAGACCTC AAAGTTCCTA
    AAGAAGGCCG GCGATGTGAT CAAAAATCTA TTGGTGCGTC TGTGGATCTG GCTACTCGTG
    CTGGTTATCT TCCTTTGCGC CATCACTGGC GAGAACATGA CCGGCTTCCG CATCTGCTAC
    ATGGCCCTGT TTCTATTCTT CTTGCTAGTC TTTCAATCTT CGTCCAAGGC GTGGGTTAAG
    ATCATGTACG GCTTCTGGCT GTTTCTGATC TTCTATGCCA TGTCCATACT TATATTGATC
    TACACATATC AATTCGACAA GTTCGACACG TACTGGAGCG ACTATCTTAA TGTGTCCGCG
    ACTTTGCAAA AGGACATCGG CCTTAAGCGC TATCAGACCA AGGATCTGTT CCTCCATTTG
    GTCTCACCGA CGATAATTGT GATCCTGACC GTCATACAAG TGCACTACTT CCACAAGCGC
    TTTATTGCAT CATTGCAGCA GCAGCCGTTG GCTGGCGGAT CGGCACAGCA GAAACCCACG
    GAGACAACTG CCTTGGAACC GGCGCCATCG AAGCGACGTG GCAGCGCCGG TTCACTGCGT
    AAATCTCAGG GTCCATCGGC GGAGGCTGCT CCAGGAGCCA CCACCGATTT CGAGACATCT
    GTGCGAGACT TGGTGCGCAT ATCGTTCCGC AAGATCAAGA ACAAGTCGGA GTACATCTTC
    AAGAACTTCA AGGATGTCTT CTGGCGCTTC CTGGAGCTGC ACATCATGAA GGCTGTGTAT
    ATCGCAGCCT TCGTGTGCAG TGTCAGCGAA GTCTGCGTAC TGCACATTAT CTTTGTGGGT
    TTCTGTGTGC TGGGCGCCAC CTCGCGGAAG GCCGTTCAGG TGGTGATCAG CCGCCTCATC
    TCGTTCATTG TCACCGTCAT AGTTCTGTCC AAGATGATCT ACCAGATCGA GTACTTGAGT
    CACTCGCAGC ACAATGTGGT TTGTTCTGAC AACCGGACTG CCAACAATGC GGAGTGGATT
    GGTCTCACCA AGGCTGACAA GGTAACTGGC GGACTGATGA GCCTGTTGCG CACCTACATC
    ATCTACATGG TTATTGTGAC CATGCACGCA GTGATCAGTT TGCGGCAGCT TCAAATGCGC
    GTCAAGATCG GAGCACTGAA TGCTCCACCC ACCAAGCTGC TGTTCCCCAA TATTATTCGA
    GCTGATGCTG AGAAGGATCT GGTGGGACTG GTCAAGTATC TCCTCAACTT TGGCTTCTAC
    AAATTCGGCA TTGAGATATC GCTAATCGCG CTGGTCTCTA CCATCACATA TCGCCAGGAT
    ATTGTTGCCG TAGTCTATGC TCTGTGGCTA GTGGTGCTTT TGCTTCTACG GAGATCGCAG
    TGCGCCAAAA TATGGGGCGT GTTTCAGGCA TTCTTTGCCA TCTCCATACT GACACAGTAT
    ATAGTGCTGG TAGGATTGCC GCCGAGCTCA TGCCTGGTGT TTCCCTGGGA TGAAGGTCCC
    TTCGGCGAGG GCATACAACG CTGGGCGATG CTGCCAGGAG CCCTGCACTT CAACCACGTA
    CCCAAGCTGA TCTTCGACTT TATTGTCTTG GTCATTCTGA ATCGACAGAA GAGTATCTTC
    TGCATCGAAC AGCGTTATGC CAGTAACGAT GACTACCCGG GTGGCAGCAA TCGCAGTGTG
    ATCGCGGATA TTGCTCAGCT AGGTCGCGTT CCCTTCGACA ATCCCACCCA CGACTTTTGT
    TCGTACATAC GCAACTACTC GGACATCCTT AAGAACGGAG TGCTGTGCGG CTTCTACTGG
    TTTACTCTGG CAGTTGTTTT CTTGGCCGGC ACCAATATTG CTGATCTGCT GGCACTGGGT
    TATCTGATCG GAGCGTTTAT CTTCCTGTGG CAGGGATCGG ATTTCTATCT GCGTCCCATA
    CACACCATCA TCTTTCGCTG GAAGTGGCTG CTGGCATTCA ATGTGGCCAA CATACTTATC
    AAGACGTCCT TCCAAATGGC CGGCTGTTTG TTCATGACCC AACTGACGAA AGACTGCTGC
    TGGCTGGTGC ACATGCTCGG CATCACATGT ACGAGCAATG TACTTACAGA GCAAATAATG
    CTGCCCGAGG AGGCTGAATT GGCGCTTAAG CCAGGCGAAT GTCCTAAGAT CACCCACCAG
    GTGGTCCTTC TGTGGGACAC GATTTGCTTT GCCTTCATCA TCTTCCAGCT GCGCATCTTC
    AAGTCGCACT ACTTCTGTCA CATCATAACG GACACCAAAG CAAATAACAT CCTGGCCTCA
    AGAGGAGCCG ACATCATTGA GAGCCTACGA CATAAGCAGA TTGCACATCG ACACGACCAT
    GAAAAGCAGG TGCTGCATAA GATCAAGCGA AAGATGGAGC GCATCCGTGC CACGCAGCAG
    AAGATGCTAC GACCCTTGGA CAAACAAACC CACTTCGACG CTACCCGTGC TGGTGACTAC
    TATATGTTCG AGGAGATGGA CGATAAGTTT GAGCTCGACT TGATACACGA CGAGATCGAC
    TTCCTCGAGG AGGAGAACAT CACCGAGAGC GAGATGAAGA TGCAGCGACG CAAGACCCTC
    TACGATAAGT CGAAGGATGC ACCCACAGGC GAGTTTCCCT CCACCAGTAA GGGTATTTCG
    AAGGAGCGCG ATGCGGCAAC GGCTTCCAGT TCGGCTAGTC CAGCGCCCAC TAGGGATGTG
    GGTGATCTGC CTGTGATTCC ACCACCCTCC ACTGGCCTGG GACGTGAGCA AACCTCCAAG
    GAGACCTCCG ATAGCAAGTC CAAAATGGAA GTGGACAGCG GAGAGGTGAC GGCCAAGGAT
    TCGGATGAGG ACTTCGATAC CAATCCCATT ATCAGACTGC TCGAGGGCTT CTTGGTCACG
    CTGACCATAA GACTGAACCG CTTTTCGCGC AACTATCGCT TTGTGAATCG CATCCTGGCC
    GGCGAAAAGA AGACCCTAAA GGAGTCCAGT TCGTTGAACC GTCTGGGGCT GTCCAGTGCC
    GCTGCCATGT TCCACTTCCT CAAATCGAAT TTAGAGAGCG ATGAAAGTGA ACCGCCCGCC
    TCCTCATCCA CCCCGCGGCG GGTGGTGATC GCACCACCGA ATGCCACCGA GCACTCAGAC
    CCCACCAGCA CCACACTGAA CACGAACACG ACAACCACAC CGCTATCACC ACCAGAACCA
    CTGCAACCAC TGCAACCACT GCAACCAAAT ACAACCAGTA CACCACAGCA ACAGCATCAG
    CATATTCGCG CTGCCGAAGA AATCATCGAA CTGCCTGTAG ATACCGTGGA TGGAGTCGCC
    CATAGAAAAC AATCAATCAA TTCATCGCCG CCAGCCAAGG GCGCGGGTGA ATTCAATCTG
    GAGGAGGAGA ACTTCGCCCA GAGAGATCAT CACATCATTG TCGAGGTGTT GATCTCCTCG
    TGGTATGCCC TGTTGGCCAA CACGGATCTC ATCTGCTACA TTGTGGTTTT CATCAATCAG
    GTGGTCAATG CCAGTCTCAT TTCGCTGCCG CTGCCCATTA TGGTCTTTTT GTGGGGCACA
    TTGTCTCTGC CGCGTCCCAC TAAAACCTTC TGGGTCACGC TGATTGCCTA CACCCAGGCC
    ATCGTGCTGA TCAAGTGCAT CTTCCAGTTT AAGCTGATCT GGTCCAATTA CCACCAACTG
    CCCAATCAGC CGCTAACACC GGCCAAAATA TTCGGCGTTG AGAATAAGGC CCATTATGCG
    ATTTACGACC TGATACTATT GCTGGTTCTA TTCCTGCATC GCTATTTGCT TAAATCACAA
    GGCCTGTGGA AATCGGGCTA CAAGGACACG GATAACCAGT TCACCAAGCC CACCGCTAGC
    ATTGATGAAC GCGACGATAG CGACAACTTA TCCCAACCGG ATTCCCGCCA GCTAAACGAT
    GATGCTGCTC AGAAGCTGAG TCTCCAAGTG AGCCAGGCTT CCTTGCCAGG ATCGCCCGAG
    TTTAGCAAGA CAGGCATCAA TCAGCTAGAG CGCACCAAGT ACACTTCATC GCTGTACAAA
    TTCTTCTTCA GTTTGGTCCA CAAATCCCGT CTAGCCACAG ATGTTTACGC TCTGATGTTC
    CTCTGCGATT TTGTGAACTT CTTCGTGCTT CTTTTCGGCT TCACTGCTTT TGGAACTCAG
    CAAACGGAAA GTGATGAAGG TGTGCAGACA TATCTTGCGG AGAACAAAGT GCCCATACCA
    TTCCTGATTA TGCTACTTGT TCAGTTCCTG CTCATTGTCA TTGATCGAGC TTTGTATCTG
    CGCAAAGCCC TGGTGAACAA GATCATCTTC CACTTCTTTT CGGTTATCGG AATACACATC
    TGGATGTTCT TCGTTGTGCC TGCAGTTACG GAGCGCACTT TCAACTCCCT CGCACCTCCA
    ATAATATTCT ACGTTATCAA GTGCTTTTAC ATGCTGCTCA GCTCTTATCA AATCAAATCC
    GGTTACCCTA AGCGCATTCT AGGCAACTTC TTCACCAAGG GTTTCTCGAT GGTCAATATG
    ATTGCCTTTA AGGTGTACAT GCAGATTCCA TTCCTGTACG AGCTGCGAAC CATATTAGAT
    TGGGTGTGCA TTGACAGCAC CATGACCATT TTCGATTGGC TAAAGATGGA GGACATTTTC
    TCGAATATAT ATCTTATCCG CTGCACCCGG CAGTCAGAGA CTGATTTTCC GGCCATGAGA
    GCTCAGAAGA AGGCCTCACT TTCAAAGCTG ATAATGGGTG GCACCATCGT CCTGCTGATA
    GTGATTTGCA TTTGGGGACC ATTGTGTTTG TTTGCACTTG GCAACGCGGT GGGCACCTCG
    AATGTGCCGT TCCATGTGTC GCTATCAATA CGAATTGGAC CCTATGATCC CATCTACACA
    ACGAACAACT ACGACAGTAT CTTCGAGATC AATCCTGAGA TGTACTCTCA GATGACTAAT
    GCATACATAA AGGAGAAACA GGCTTTGACG TTTATTGCCG GCTATGATGC AACGGATGTA
    GCGGCGGTTA GACTAGCTGG CAACTCGCCA TCTCTGTGGA ACATAGCACC GCCGGATAGG
    CAGCGACTAC TAAATGATTT AAGAAACAAT CACACGCTGA AGGCCCGGTT CTCTTATTCC
    CTCACCCGAA AGGCTCCTGC CAAGGGGCTA AAGGAAAATG TGGGCGACGA GCATGCCATA
    TCGCTGGACG AGTCCTTTGA GGGACGAGCA GCACTCATTC ACATGCTAAG CGAAACCCAT
    GATGTGGAGC CCATTTACAG CAATGGCACC ACAAATGGAA CCACTCCCGA AGTTGAAGAA
    GTGGTGGTGA TACCCGGAAT GATACCCAAG TTTATCAAGG TTCTCAATTC GGGCGACGCT
    GCGGTGGTAA GTGTTTTGAG CCCAAAACAC TACGACTACC GACCGTTGGT TATCAAAATG
    CATCGGGACA ACGAGACCAA TGGGTTGTGG TGGGAGATTC GGGACTATTG CAACGACACC
    TTCTACAACG AAACTCTATC GAAGTTTGCA TACAGCAACT GCACTTCGGG AATTGTGATG
    TACACGTTCA ACGATAAAAA GTTTCCATCC ACGTTCAGTT TCCTCACAGC GGGAGGCATC
    ATTGGTCTGT ACACTACCTT TGTGTTATTG GCCTCGCGCT TTATGAAGTC CTTCATTGGC
    GGGCAAAACA GAAAGATCAT GTTTGAGGAT CTGCCCTATG TTGATAGAGT GCTGCAGCTC
    TGTCTGGACA TTTATCTGGT ACGGGAGGCA TTGGAATTCG CTTTGGAAGA GGACCTGTTT
    GCCAAATTAC TCTTCCTATA CCGATCGCCC GAAACGCTAA TCAAGTGGAC GCGTCCCAAG
    GAGGAGTACG TGGACGATGA TGGCGACACC GACTCGATTC CCAGTCGAAT GAGCGTGCGC
    CGGCCGGAGC AACTGCAGCC ACAGCAACCG CAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001260227.1
    CDS577..8232
    Translation

    Target ORF information:

    RefSeq Version NM_001273298.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster piezo, transcript variant L (Piezo), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001273298.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGTCTTCA GCTATGCGTG CATGGTGCTC CAGCGCATCG TGGTGCCAGC GGTTCTGGTA 
    CTCGCTGCCC TGATGCGACC AGTGGGCATA TCCTTTGTCT ACCTTTTGAT GTTCTTTGTG
    TCGCCCTTTG TTCCGCTGGC CACGCGACGC AACTTCAAAG GATCTGTGAC TGCCTTCTTC
    ATCATCCTGC TGACGTTGAG CACGCTGGTC CTCTTGGGTC ACATAACGCT ACAGATTCTG
    GCGGTCAGCC TCACGCTGCC CATCTACAAT TGCTCGTTCA GTGAGCGTCT GCTGCGTCAC
    ATTGGCTTCG TGAGCTTTAT TGATCTACAG CCATTTGCCA TCATCGAATG GCTAGTGCCT
    GAGGTGTTGG TTTTCGCCAC CTCCCTGGGT TCGTATCTCA CGGTGAAGCG AGTGGCCTCA
    CAGCCCGTCG GCGCCGAGCA GCTGGAGAAC GGCGAGGTGG TCGATGGCCA GGCGGAAAAC
    GCGCAGACAT CTTCTCAGCC ATCTGCCGCA GATGCCAATG GAGGAGATGT GCAACAGGCC
    ACGGTCACCA CGCCGCTGCA GCAACAGCAG CAGCAGCTGA GGAAACGAGT GTCCATGATC
    AGCCAGCACA TACACTTTGA GGGCTTGGTC AAGATCTCTC CTCTGTTCTG CCTGGCCACG
    CTGTTCTTTG CGGCCGTACT GCGTCCCTCG GTGCCTGGCG GATTTTACTT TCTCATTTTC
    CTGCTGTCCG GCACCTACTG GGCAACATGC CAGACGCTGC AACGGGGCTT CGCTTTGTTG
    CTGCGCTGCG TGATGGTCGT CCTCGTGCTG CACTCCCTGT CCATTGTGTC ATACCAGACG
    CCATGGATGC AGAGCCACCT CAATCATACC ACCCTGACAG CTCGATTGAT TGGACTGGAA
    CCGCTTATTG AATCCTACTG CTCGCCGGAT ATACGAGTCT TTCTGTACAA TAATAAGCTA
    TCCCTGGACT CGTACCTCAA TCCCTTTGCG TTGTTCTTTG CCTATTTCGC ACTGGCACTG
    ACCACCAAGC ATCTGATTAA GCCACGGGTT TCTTTGCGCC GAGATCAGCG TGCAACGTTA
    AATGAACCGA CTGAGACGAC GCCTTTGGTG CGGCAAAGCA CGCGAAAGGC ACGTACACCC
    CAACCGCTGG AAAGTGGATC CTCGGTGGCG CCAAGTGTCA CTCAGCGGGG CAACGACATG
    CAGCTGGAAT CGATGGAGCA GCGATCGGAA CAGGAGAACA CCACCACATC CATACTGGAT
    CAAATATCGT ATGGATTCGT CAGCGTGGGA GGATTCATAT ATCAGAATAG CTATATATTC
    ACCAATATTC TTATGATGGC CTGGTCCATA GTGTATCACA GTTGGCTGAC TTTTGTCCTC
    TTGCTGTCGG CCAACGTACT GTGGATGATT CCCAACCAGA GGAAGGCCAT GATGCGGTCC
    AGTCCCTTTA TAGTTCTGTA TGCTGAGGCC CTTTTGATTG CCCAATACAT ATACGGCATG
    GATCTCAACA ACGAAGAGCT GCCCACGAGC GTTCCCTATT TGCAGACTGC GGGCATTAAC
    CTGCAACAAA TTGGCTTCGA ACGACCCATC GAGAACCAAA TGCGGCCATG TGTGCCGCTG
    ATCGTGAAGA CAGCGTTTGT GCTAATGTTT TGGGTGACAT CACGACAGTT CTTCAAGGAG
    AAGCGCGATC GCCGACGGGA CAGCACTCTG GCGGACTTCA TTGCCCCACT GCAGATTACT
    GTGGGATCGG CTGGCTCCAG CTACCTCATC AACGATGGCA AGAAGACCTC AAAGTTCCTA
    AAGAAGGCCG GCGATGTGAT CAAAAATCTA TTGGTGCGTC TGTGGATCTG GCTACTCGTG
    CTGGTTATCT TCCTTTGCGC CATCACTGGC GAGAACATGA CCGGCTTCCG CATCTGCTAC
    ATGGCCCTGT TTCTATTCTT CTTGCTAGTC TTTCAATCTT CGTCCAAGGC GTGGGTTAAG
    ATCATGTACG GCTTCTGGCT GTTTCTGATC TTCTATGCCA TGTCCATACT TATATTGATC
    TACACATATC AATTCGACAA GTTCGACACG TACTGGAGCG ACTATCTTAA TGTGTCCGCG
    ACTTTGCAAA AGGACATCGG CCTTAAGCGC TATCAGACCA AGGATCTGTT CCTCCATTTG
    GTCTCACCGA CGATAATTGT GATCCTGACC GTCATACAAG TGCACTACTT CCACAAGCGC
    TTTATTGCAT CATTGCAGCA GCAGCCGTTG GCTGGCGGAT CGGCACAGCA GAAACCCACG
    GAGACAACTG CCTTGGAACC GGCGCCATCG AAGCGACGTG GCAGCGCCGG TTCACTGCGT
    AAATCTCAGG GTCCATCGGC GGAGGCTGCT CCAGGAGCCA CCACCGATTT CGAGACATCT
    GTGCGAGACT TGGTGCGCAT ATCGTTCCGC AAGATCAAGA ACAAGTCGGA GTACATCTTC
    AAGAACTTCA AGGATGTCTT CTGGCGCTTC CTGGAGCTGC ACATCATGAA GGCTGTGTAT
    ATCGCAGCCT TCGTGTGCAG TGTCAGCGAA GTCTGCGTAC TGCACATTAT CTTTGTGGGT
    TTCTGTGTGC TGGGCGCCAC CTCGCGGAAG GCCGTTCAGG TGGTGATCAG CCGCCTCATC
    TCGTTCATTG TCACCGTCAT AGTTCTGTCC AAGATGATCT ACCAGATCGA GTACTTGAGT
    CACTCGCAGC ACAATGTGGT TTGTTCTGAC AACCGGACTG CCAACAATGC GGAGTGGATT
    GGTCTCACCA AGGCTGACAA GGTAACTGGC GGACTGATGA GCCTGTTGCG CACCTACATC
    ATCTACATGG TTATTGTGAC CATGCACGCA GTGATCAGTT TGCGGCAGCT TCAAATGCGC
    GTCAAGATCG GAGCACTGAA TGCTCCACCC ACCAAGCTGC TGTTCCCCAA TATTATTCGA
    GCTGATGCTG AGAAGGATCT GGTGGGACTG GTCAAGTATC TCCTCAACTT TGGCTTCTAC
    AAATTCGGCA TTGAGATATC GCTAATCGCG CTGGTCTCTA CCATCACATA TCGCCAGGAT
    ATTGTTGCCG TAGTCTATGC TCTGTGGCTA GTGGTGCTTT TGCTTCTACG GAGATCGCAG
    TGCGCCAAAA TATGGGGCGT GTTTCAGGCA TTCTTTGCCA TCTCCATACT GACACAGTAT
    ATAGTGCTGG TAGGATTGCC GCCGAGCTCA TGCCTGGTGT TTCCCTGGGA TGAAGGTCCC
    TTCGGCGAGG GCATACAACG CTGGGCGATG CTGCCAGGAG CCCTGCACTT CAACCACGTA
    CCCAAGCTGA TCTTCGACTT TATTGTCTTG GTCATTCTGA ATCGACAGAA GAGTATCTTC
    TGCATCGAAC AGCGTTATGC CAGTAACGAT GACTACCCGG GTGGCAGCAA TCGCAGTGTG
    ATCGCGGATA TTGCTCAGCT AGGTCGCGTT CCCTTCGACA ATCCCACCCA CGACTTTTGT
    TCGTACATAC GCAACTACTC GGACATCCTT AAGAACGGAG TGCTGTGCGG CTTCTACTGG
    TTTACTCTGG CAGTTGTTTT CTTGGCCGGC ACCAATATTG CTGATCTGCT GGCACTGGGT
    TATCTGATCG GAGCGTTTAT CTTCCTGTGG CAGGGATCGG ATTTCTATCT GCGTCCCATA
    CACACCATCA TCTTTCGCTG GAAGTGGCTG CTGGCATTCA ATGTGGCCAA CATACTTATC
    AAGACGTCCT TCCAAATGGC CGGCTGTTTG TTCATGACCC AACTGACGAA AGACTGCTGC
    TGGCTGGTGC ACATGCTCGG CATCACATGT ACGAGCAATG TACTTACAGA GCAAATAATG
    CTGCCCGAGG AGGCTGAATT GGCGCTTAAG CCAGGCGAAT GTCCTAAGAT CACCCACCAG
    GTGGTCCTTC TGTGGGACAC GATTTGCTTT GCCTTCATCA TCTTCCAGCT GCGCATCTTC
    AAGTCGCACT ACTTCTGTCA CATCATAACG GACACCAAAG CAAATAACAT CCTGGCCTCA
    AGAGGAGCCG ACATCATTGA GAGCCTACGA CATAAGCAGA TTGCACATCG ACACGACCAT
    GAAAAGCAGG TGCTGCATAA GATCAAGCGA AAGATGGAGC GCATCCGTGC CACGCAGCAG
    AAGATGCTAC GACCCTTGGA CAAACAAACC CACTTCGACG CTACCCGTGC TGGTGACTAC
    TATATGTTCG AGGAGATGGA CGATAAGTTT GAGCTCGACT TGATACACGA CGAGATCGAC
    TTCCTCGAGG AGGAGAACAT CACCGAGAGC GAGATGAAGA TGCAGCGACG CAAGACCCTC
    TACGATAAGT CGAAGGATGC ACCCACAGGC GAGTTTCCCT CCACCAGTAA GGGTATTTCG
    AAGGAGCGCG ATGCGGCAAC GGCTTCCAGT TCGGCTAGTC CAGCGCCCAC TAGGGATGTG
    GGTGATCTGC CTGTGATTCC ACCACCCTCC ACTGGCCTGG GACGTGAGCA AACCTCCAAG
    GAGACCTCCG ATAGCAAGTC CAAAATGGAA GTGGACAGCG GAGAGGTGAC GGCCAAGGAT
    TCGGATGAGG ACTTCGATAC CAATCCCATT ATCAGACTGC TCGAGGGCTT CTTGGTCACG
    CTGACCATAA GACTGAACCG CTTTTCGCGC AACTATCGCT TTGTGAATCG CATCCTGGCC
    GGCGAAAAGA AGACCCTAAA GGAGTCCAGT TCGTTGAACC GTCTGGGGCT GTCCAGTGCC
    GCTGCCATGT TCCACTTCCT CAAATCGAAT TTAGAGAGCG ATGAAAGTGA ACCGCCCGCC
    TCCTCATCCA CCCCGCGGCG GGTGGTGATC GCACCACCGA ATGCCACCGA GCACTCAGAC
    CCCACCAGCA CCACACTGAA CACGAACACG ACAACCACAC CGCTATCACC ACCAGAACCA
    CTGCAACCAC TGCAACCACT GCAACCAAAT ACAACCAGTA CACCACAGCA ACAGCATCAG
    CATATTCGCG CTGCCGAAGA AATCATCGAA CTGCCTGTAG ATACCGTGGA TGGAGTCGCC
    CATAGAAAAC AATCAATCAA TTCATCGCCG CCAGCCAAGG GCGCGGGTGA ATTCAATCTG
    GAGGAGGAGA ACTTCGCCCA GAGAGATCAT CACATCATTG TCGAGGTGTT GATCTCCTCG
    TGGTATGCCC TGTTGGCCAA CACGGATCTC ATCTGCTACA TTGTGGTTTT CATCAATCAG
    GTGGTCAATG CCAGTCTCAT TTCGCTGCCG CTGCCCATTA TGGTCTTTTT GTGGGGCACA
    TTGTCTCTGC CGCGTCCCAC TAAAACCTTC TGGGTCACGC TGATTGCCTA CACCCAGGCC
    ATCGTGCTGA TCAAGTGCAT CTTCCAGTTT AAGCTGATCT GGTCCAATTA CCACCAACTG
    CCCAATCAGC CGCTAACACC GGCCAAAATA TTCGGCGTTG AGAATAAGGC CCATTATGCG
    ATTTACGACC TGATACTATT GCTGGTTCTA TTCCTGCATC GCTATTTGCT TAAATCACAA
    GGCCTGTGGA AATCGGGCTA CAAGGACACG GATAACCAGT TCACCAAGCC CACCGCTAGC
    ATTGATGAAC GCGACGATAG CGACAACTTA TCCCAACCGG ATTCCCGCCA GCTAAACGAT
    GATGCTGCTC AGAAGCTGAG TCTCCAAGTG AGCCAGGCTT CCTTGCCAGG ATCGCCCGAG
    TTTAGCAAGA CAGGCATCAA TCAGCTAGAG CGCACCAAGT ACACTTCATC GCTGTACAAA
    TTCTTCTTCA GTTTGGTCCA CAAATCCCGT CTAGCCACAG ATGTTTACGC TCTGATGTTC
    CTCTGCGATT TTGTGAACTT CTTCGTGCTT CTTTTCGGCT TCACTGCTTT TGGAACTCAG
    CAAACGGAAA GTGATGAAGG TGTGCAGACA TATCTTGCGG AGAACAAAGT GCCCATACCA
    TTCCTGATTA TGCTACTTGT TCAGTTCCTG CTCATTGTCA TTGATCGAGC TTTGTATCTG
    CGCAAAGCCC TGGTGAACAA GATCATCTTC CACTTCTTTT CGGTTATCGG AATACACATC
    TGGATGTTCT TCGTTGTGCC TGCAGTTACG GAGCGCACTT TCAACTCCCT CGCACCTCCA
    ATAATATTCT ACGTTATCAA GTGCTTTTAC ATGCTGCTCA GCTCTTATCA AATCAAATCC
    GGTTACCCTA AGCGCATTCT AGGCAACTTC TTCACCAAGG GTTTCTCGAT GGTCAATATG
    ATTGCCTTTA AGGTGTACAT GCAGATTCCA TTCCTGTACG AGCTGCGAAC CATATTAGAT
    TGGGTGTGCA TTGACAGCAC CATGACCATT TTCGATTGGC TAAAGATGGA GGACATTTTC
    TCGAATATAT ATCTTATCCG CTGCACCCGG CAGTCAGAGA CTGATTTTCC GGCCATGAGA
    GCTCAGAAGA AGGCCTCACT TTCAAAGCTG ATAATGGGTG GCACCATCGT CCTGCTGATA
    GTGATTTGCA TTTGGGGACC ATTGTGTTTG TTTGCACTTG GCAACGCGGT GGGCACCTCG
    AATGTGCCGT TCCATGTGTC GCTATCAATA CGAATTGGAC CCTATGATCC CATCTACACA
    ACGAACAACT ACGACAGTAT CTTCGAGATC AATCCTGAGA TGTACTCTCA GATGACTAAT
    GCATACATAA AGGAGAAACA GGCTTTGACG TTTATTGCCG GCTATGATGC AACGGATGTA
    GCGGCGGTTA GACTAGCTGG CAACTCGCCA TCTCTGTGGA ACATAGCACC GCCGGATAGG
    CAGCGACTAC TAAATGATTT AAGAAACAAT CACACGCTGA AGGCCCGGTT CTCTTATTCC
    CTCACCCGAA AGGCTCCTGC CAAGGGGCTA AAGGAAAATG TGGGCGACGA GCATGCCATA
    TCGCTGGACG AGTCCTTTGA GGGACGAGCA GCACTCATTC ACATGCTAAG CGAAACCCAT
    GATGTGGAGC CCATTTACAG CAATGGCACC ACAAATGGAA CCACTCCCGA AGTTGAAGAA
    GTGGTGGTGA TACCCGGAAT GATACCCAAG TTTATCAAGG TTCTCAATTC GGGCGACGCT
    GCGGTGGTAA GTGTTTTGAG CCCAAAACAC TACGACTACC GACCGTTGGT TATCAAAATG
    CATCGGGACA ACGAGACCAA TGGGTTGTGG TGGGAGATTC GGGACTATTG CAACGACACC
    TTCTACAACG AAACTCTATC GAAGTTTGCA TACAGCAACT GCACTTCGGG AATTGTGATG
    TACACGTTCA ACGATAAAAA GTTTCCATCC ACGTTCAGTT TCCTCACAGC GGGAGGCATC
    ATTGGTCTGT ACACTACCTT TGTGTTATTG GCCTCGCGCT TTATGAAGTC CTTCATTGGC
    GGGCAAAACA GAAAGATCAT GTTTGAGGAT CTGCCCTATG TTGATAGAGT GCTGCAGCTC
    TGTCTGGACA TTTATCTGGT ACGGGAGGCA TTGGAATTCG CTTTGGAAGA GGACCTGTTT
    GCCAAATTAC TCTTCCTATA CCGATCGCCC GAAACGCTAA TCAAGTGGAC GCGTCCCAAG
    GAGGAGTACG TGGACGATGA TGGCGACACC GACTCGATTC CCAGTCGAAT GAGCGTGCGC
    CGGCCGGAGC AACTGCAGCC ACAGCAACCG CAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa15939
    Clone ID Related Accession (Same CDS sequence) NM_001201789.2
    Accession Version NM_001201789.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7713bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product piezo, isoform H
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001201789.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGTCTTCA GCTATGCGTG CATGGTGCTC CAGCGCATCG TGGTGCCAGC GGTTCTGGTA 
    CTCGCTGCCC TGATGCGACC AGTGGGCATA TCCTTTGTCT ACCTTTTGAT GTTCTTTGTG
    TCGCCCTTTG TTCCGCTGGC CACGCGACGC AACTTCAAAG GATCTGTGAC TGCCTTCTTC
    ATCATCCTGC TGACGTTGAG CACGCTGGTC CTCTTGGGTC ACATAACGCT ACAGATTCTG
    GCGGTCAGCC TCACGCTGCC CATCTACAAT TGCTCGTTCA GTGAGCGTCT GCTGCGTCAC
    ATTGGCTTCG TGAGCTTTAT TGATCTACAG CCATTTGCCA TCATCGAATG GCTAGTGCCT
    GAGGTGTTGG TTTTCGCCAC CTCCCTGGGT TCGTATCTCA CGGTGAAGCG AGTGGCCTCA
    CAGCCCGTCG GCGCCGAGCA GCTGGAGAAC GGCGAGGTGG TCGATGGCCA GGCGGAAAAC
    GCGCAGACAT CTTCTCAGCC ATCTGCCGCA GATGCCAATG GAGGAGATGT GCAACAGGCC
    ACGGTCACCA CGCCGCTGCA GCAACAGCAG CAGCAGCTGA GGAAACGAGT GTCCATGATC
    AGCCAGCACA TACACTTTGA GGGCTTGGTC AAGATCTCTC CTCTGTTCTG CCTGGCCACG
    CTGTTCTTTG CGGCCGTACT GCGTCCCTCG GTGCCTGGCG GATTTTACTT TCTCATTTTC
    CTGCTGTCCG GCACCTACTG GGCAACATGC CAGACGCTGC AACGGGGCTT CGCTTTGTTG
    CTGCGCTGCG TGATGGTCGT CCTCGTGCTG CACTCCCTGT CCATTGTGTC ATACCAGACG
    CCATGGATGC AGAGCCACCT CAATCATACC ACCCTGACAG CTCGATTGAT TGGACTGGAA
    CCGCTTATTG AATCCTACTG CTCGCCGGAT ATACGAGTCT TTCTGTACAA TAATAAGCTA
    TCCCTGGACT CGTACCTCAA TCCCTTTGCG TTGTTCTTTG CCTATTTCGC ACTGGCACTG
    ACCACCAAGC ATCTGATTAA GCCACGGGTT TCTTTGCGCC GAGATCAGCG TGCAACGTTA
    AATGAACCGA CTGAGACGAC GCCTTTGGTG CGGCAAAGCA CGCGAAAGGC ACGTACACCC
    CAACCGCTGG AAAGTGGATC CTCGGTGGCG CCAAGTGTCA CTCAGCGGGG CAACGACATG
    CAGCTGGAAT CGATGGAGCA GCGATCGGAA CAGGAGAACA CCACCACATC CATACTGGAT
    CAAATATCGT ATGGATTCGT CAGCGTGGGA GGATTCATAT ATCAGAATAG CTATATATTC
    ACCAATATTC TTATGATGGC CTGGTCCATA GTGTATCACA GTTGGCTGAC TTTTGTCCTC
    TTGCTGTCGG CCAACGTACT GTGGATGATT CCCAACCAGA GGAAGGCCAT GATGCGGTCC
    AGTCCCTTTA TAGTTCTGTA TGCTGAGGCC CTTTTGATTG CCCAATACAT ATACGGCATG
    GATCTCAACA ACGAAGAGCT GCCCACGAGC GTTCCCACTG CGGGCATTAA CCTGCAACAA
    ATTGGCTTCG AACGACCCAT CGAGAACCAA ATGCGGCCAT GTGTGCCGCT GATCGTGAAG
    ACAGCGTTTG TGCTAATGTT TTGGGTGACA TCACGACAGT TCTTCAAGGA GAAGCGCGAT
    CGCCGACGGG ACAGCACTCT GGCGGACTTC ATTGCCCCAC TGCAGATTAC TGTGGGATCG
    GCTGGCTCCA GCTACCTCAT CAACGATGGC AAGAAGACCT CAAAGTTCCT AAAGAAGGCC
    GGCGATGTGA TCAAAAATCT ATTGGTGCGT CTGTGGATCT GGCTACTCGT GCTGGTTATC
    TTCCTTTGCG CCATCACTGG CGAGAACATG ACCGGCTTCC GCATCTGCTA CATGGCCCTG
    TTTCTATTCT TCTTGCTAGT CTTTCAATCT TCGTCCAAGG CGTGGGTTAA GATCATGTAC
    GGCTTCTGGC TGTTTCTGAT CTTCTATGCC ATGTCCATAC TTATATTGAT CTACACATAT
    CAATTCGACA AGTTCGACAC GTACTGGAGC GACTATCTTA ATGTGTCCGC GACTTTGCAA
    AAGGACATCG GCCTTAAGCG CTATCAGACC AAGGATCTGT TCCTCCATTT GGTCTCACCG
    ACGATAATTG TGATCCTGAC CGTCATACAA GTGCACTACT TCCACAAGCG CTTTATTGCA
    TCATTGCAGC AGCAGCCGTT GGCTGGCGGA TCGGCACAGC AGAAACCCAC GGAGACAACT
    GCCTTGGAAC CGGCGCCATC GAAGCGACGT GGCAGCGCCG GTTCACTGCG TAAATCTCAG
    GGTCCATCGG CGGAGGCTGC TCCAGGAGCC ACCACCGATT TCGAGACATC TGTGCGAGAC
    TTGGTGCGCA TATCGTTCCG CAAGATCAAG AACAAGTCGG AGTACATCTT CAAGAACTTC
    AAGGATGTCT TCTGGCGCTT CCTGGAGCTG CACATCATGA AGGCTGTGTA TATCGCAGCC
    TTCGTGTGCA GTGTCAGCGA AGTCTGCGTA CTGCACATTA TCTTTGTGGG TTTCTGTGTG
    CTGGGCGCCA CCTCGCGGAA GGCCGTTCAG GTGGTGATCA GCCGCCTCAT CTCGTTCATT
    GTCACCGTCA TAGTTCTGTC CAAGATGATC TACCAGATCG AGTACTTGAG TCACTCGCAG
    CACAATGTGG TTTGTTCTGA CAACCGGACT GCCAACAATG CGGAGTGGAT TGGTCTCACC
    AAGGCTGACA AGGTAACTGG CGGACTGATG AGCCTGTTGC GCACCTACAT CATCTACATG
    GTTATTGTGA CCATGCACGC AGTGATCAGT TTGCGGCAGC TTCAAATGCG CGTCAAGATC
    GGAGCACTGA ATGCTCCACC CACCAAGCTG CTGTTCCCCA ATATTATTCG AGCTGATGCT
    GAGAAGGATC TGGTGGGACT GGTCAAGTAT CTCCTCAACT TTGGCTTCTA CAAATTCGGC
    ATTGAGATAT CGCTAATCGC GCTGGTCTCT ACCATCACAT ATCGCCAGGA TATTGTTGCC
    GTAGTCTATG CTCTGTGGCT AGTGGTGCTT TTGCTTCTAC GGAGATCGCA GTGCGCCAAA
    ATATGGGGCG TGTTTCAGGC ATTCTTTGCC ATCTCCATAC TGACACAGTA TATAGTGCTG
    GTAGGATTGC CGCCGAGCTC ATGCCTGGTG TTTCCCTGGG ATGAAGGTCC CTTCGGCGAG
    GGCATACAAC GCTGGGCGAT GCTGCCAGGA GCCCTGCACT TCAACCACGT ACCCAAGCTG
    ATCTTCGACT TTATTGTCTT GGTCATTCTG AATCGACAGA AGAGTATCTT CTGCATCGAA
    CAGCGTTATG CCAGTAACGA TGACTACCCG GGTGGCAGCA ATCGCAGTGT GATCGCGGAT
    ATTGCTCAGC TAGGTCGCGT TCCCTTCGAC AATCCCACCC ACGACTTTTG TTCGTACATA
    CGCAACTACT CGGACATCCT TAAGAACGGA GTGCTGTGCG GCTTCTACTG GTTTACTCTG
    GCAGTTGTTT TCTTGGCCGG CACCAATATT GCTGATCTGC TGGCACTGGG TTATCTGATC
    GGAGCGTTTA TCTTCCTGTG GCAGGGATCG GATTTCTATC TGCGTCCCAT ACACACCATC
    ATCTTTCGCT GGAAGTGGCT GCTGGCATTC AATGTGGCCA ACATACTTAT CAAGACGTCC
    TTCCAAATGG CCGGCTGTTT GTTCATGACC CAACTGACGA AAGACTGCTG CTGGCTGGTG
    CACATGCTCG GCATCACATG TACGAGCAAT GTACTTACAG AGCAAATAAT GCTGCCCGAG
    GAGGCTGAAT TGGCGCTTAA GCCAGGCGAA TGTCCTAAGA TCACCCACCA GGTGGTCCTT
    CTGTGGGACA CGATTTGCTT TGCCTTCATC ATCTTCCAGC TGCGCATCTT CAAGTCGCAC
    TACTTCTGTC ACATCATAAC GGACACCAAA GCAAATAACA TCCTGGCCTC AAGAGGAGCC
    GACATCATTG AGAGCCTACG ACATAAGCAG ATTGCACATC GACACGACCA TGAAAAGCAG
    GTGCTGCATA AGATCAAGCG AAAGATGGAG CGCATCCGTG CCACGCAGCA GAAGATGCTA
    CGACCCTTGG ACAAACAAAC CCACTTCGAC GAACATGGTT ATCCACTTCC TGCACCAACA
    GTACGCAGAA GGAAGGAAAT TAAATTACAT CCACATGCTA CCCGTGCTGG TGACTACTAT
    ATGTTCGAGG AGATGGACGA TAAGTTTGAG CTCGACTTGA TACACGACGA GATCGACTTC
    CTCGAGGAGG AGAACATCAC CGAGAGCGAG ATGAAGATGC AGCGACGCAA GACCCTCTAC
    GATAAGTCGA AGGATGCACC CACAGGCGAG TTTCCCTCCA CCAGTAAGGG TATTTCGAAG
    GAGCGCGATG CGGCAACGGC TTCCAGTTCG GCTAGTCCAG CGCCCACTAG GGATGTGGGT
    GATCTGCCTG TGATTCCACC ACCCTCCACT GGCCTGGGAC GTGAGCAAAC CTCCAAGGAG
    ACCTCCGATA GCAAGTCCAA AATGGAAGTG GACAGCGGAG AGGTGACGGC CAAGGATTCG
    GATGAGGACT TCGATACCAA TCCCATTATC AGACTGCTCG AGGGCTTCTT GGTCACGCTG
    ACCATAAGAC TGAACCGCTT TTCGCGCAAC TATCGCTTTG TGAATCGCAT CCTGGCCGGC
    GAAAAGAAGA CCCTAAAGGA GTCCAGTTCG TTGAACCGTC TGGGGCTGTC CAGTGCCGCT
    GCCATGTTCC ACTTCCTCAA ATCGAATTTA GAGAGCGATG AAAGTGAACC GCCCGCCTCC
    TCATCCACCC CGCGGCGGGT GGTGATCGCA CCACCGAATG CCACCGAGCA CTCAGACCCC
    ACCAGCACCA CACTGAACAC GAACACGACA ACCACACCGC TATCACCACC AGAACCACTG
    CAACCACTGC AACCACTGCA ACCAAATACA ACCAGTACAC CACAGCAACA GCATCAGCAT
    ATTCGCGCTG CCGAAGAAAT CATCGAACTG CCTGTAGATA CCGTGGATGG AGTCGCCCAT
    AGAAAACAAT CAATCAATTC ATCGCCGCCA GCCAAGGGCG CGGGTGAATT CAATCTGGAG
    GAGGAGAACT TCGCCCAGAG AGATCATCAC ATCATTGTCG AGGTGTTGAT CTCCTCGTGG
    TATGCCCTGT TGGCCAACAC GGATCTCATC TGCTACATTG TGGTTTTCAT CAATCAGGTG
    GTCAATGCCA GTCTCATTTC GCTGCCGCTG CCCATTATGG TCTTTTTGTG GGGCACATTG
    TCTCTGCCGC GTCCCACTAA AACCTTCTGG GTCACGCTGA TTGCCTACAC CCAGGCCATC
    GTGCTGATCA AGTGCATCTT CCAGTTTAAG CTGATCTGGT CCAATTACCA CCAACTGCCC
    AATCAGCCGC TAACACCGGC CAAAATATTC GGCGTTGAGA ATAAGGCCCA TTATGCGATT
    TACGACCTGA TACTATTGCT GGTTCTATTC CTGCATCGCT ATTTGCTTAA ATCACAAGGC
    CTGTGGAAAT CGGGCTACAA GGACACGGAT AACCAGTTCA CCAAGCCCAC CGCTAGCATT
    GATGAACGCG ACGATAGCGA CAACTTATCC CAACCGGATT CCCGCCAGCT AAACGATGAT
    GCTGCTCAGA AGCTGAGTCT CCAAGTGAGC CAGGCTTCCT TGCCAGGATC GCCCGAGTTT
    AGCAAGACAG GCATCAATCA GCTAGAGCGC ACCAAGTACA CTTCATCGCT GTACAAATTC
    TTCTTCAGTT TGGTCCACAA ATCCCGTCTA GCCACAGATG TTTACGCTCT GATGTTCCTC
    TGCGATTTTG TGAACTTCTT CGTGCTTCTT TTCGGCTTCA CTGCTTTTGG AACTCAGCAA
    ACGGAAAGTG ATGAAGGTGT GCAGACATAT CTTGCGGAGA ACAAAGTGCC CATACCATTC
    CTGATTATGC TACTTGTTCA GTTCCTGCTC ATTGTCATTG ATCGAGCTTT GTATCTGCGC
    AAAGCCCTGG TGAACAAGAT CATCTTCCAC TTCTTTTCGG TTATCGGAAT ACACATCTGG
    ATGTTCTTCG TTGTGCCTGC AGTTACGGAG CGCACTTTCA ACTCCCTCGC ACCTCCAATA
    ATATTCTACG TTATCAAGTG CTTTTACATG CTGCTCAGCT CTTATCAAAT CAAATCCGGT
    TACCCTAAGC GCATTCTAGG CAACTTCTTC ACCAAGGGTT TCTCGATGGT CAATATGATT
    GCCTTTAAGG TGTACATGCA GATTCCATTC CTGTACGAGC TGCGAACCAT ATTAGATTGG
    GTGTGCATTG ACAGCACCAT GACCATTTTC GATTGGCTAA AGATGGAGGA CATTTTCTCG
    AATATATATC TTATCCGCTG CACCCGGCAG TCAGAGACTG ATTTTCCGGC CATGAGAGCT
    CAGAAGAAGG CCTCACTTTC AAAGCTGATA ATGGGTGGCA CCATCGTCCT GCTGATAGTG
    ATTTGCATTT GGGGACCATT GTGTTTGTTT GCACTTGGCA ACGCGGTGGG CACCTCGAAT
    GTGCCGTTCC ATGTGTCGCT ATCAATACGA ATTGGACCCT ATGATCCCAT CTACACAACG
    AACAACTACG ACAGTATCTT CGAGATCAAT CCTGAGATGT ACTCTCAGAT GACTAATGCA
    TACATAAAGG AGAAACAGGC TTTGACGTTT ATTGCCGGCT ATGATGCAAC GGATGTAGCG
    GCGGTTAGAC TAGCTGGCAA CTCGCCATCT CTGTGGAACA TAGCACCGCC GGATAGGCAG
    CGACTACTAA ATGATTTAAG AAACAATCAC ACGCTGAAGG CCCGGTTCTC TTATTCCCTC
    ACCCGAAAGG CTCCTGCCAA GGGGCTAAAG GAAAATGTGG GCGACGAGCA TGCCATATCG
    CTGGACGAGT CCTTTGAGGG ACGAGCAGCA CTCATTCACA TGCTAAGCGA AACCCATGAT
    GTGGAGCCCA TTTACAGCAA TGGCACCACA AATGGAACCA CTCCCGAAGT TGAAGAAGTG
    GTGGTGATAC CCGGAATGAT ACCCAAGTTT ATCAAGGTTC TCAATTCGGG CGACGCTGCG
    GTGGTAAGTG TTTTGAGCCC AAAACACTAC GACTACCGAC CGTTGGTTAT CAAAATGCAT
    CGGGACAACG AGACCAATGG GTTGTGGTGG GAGATTCGGG ACTATTGCAA CGACACCTTC
    TACAACGAAA CTCTATCGAA GTTTGCATAC AGCAACTGCA CTTCGGGAAT TGTGATGTAC
    ACGTTCAACG ATAAAAAGTT TCCATCCACG TTCAGTTTCC TCACAGCGGG AGGCATCATT
    GGTCTGTACA CTACCTTTGT GTTATTGGCC TCGCGCTTTA TGAAGTCCTT CATTGGCGGG
    CAAAACAGAA AGATCATGTT TGAGGATCTG CCCTATGTTG ATAGAGTGCT GCAGCTCTGT
    CTGGACATTT ATCTGGTACG GGAGGCATTG GAATTCGCTT TGGAAGAGGA CCTGTTTGCC
    AAATTACTCT TCCTATACCG ATCGCCCGAA ACGCTAATCA AGTGGACGCG TCCCAAGGAG
    GAGTACGTGG ACGATGATGG CGACACCGAC TCGATTCCCA GTCGAATGAG CGTGCGCCGG
    CCGGAGCAAC TGCAGCCACA GCAACCGCAA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001188718.1
    CDS577..8289
    Translation

    Target ORF information:

    RefSeq Version NM_001201789.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster piezo, transcript variant H (Piezo), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001201789.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGTCTTCA GCTATGCGTG CATGGTGCTC CAGCGCATCG TGGTGCCAGC GGTTCTGGTA 
    CTCGCTGCCC TGATGCGACC AGTGGGCATA TCCTTTGTCT ACCTTTTGAT GTTCTTTGTG
    TCGCCCTTTG TTCCGCTGGC CACGCGACGC AACTTCAAAG GATCTGTGAC TGCCTTCTTC
    ATCATCCTGC TGACGTTGAG CACGCTGGTC CTCTTGGGTC ACATAACGCT ACAGATTCTG
    GCGGTCAGCC TCACGCTGCC CATCTACAAT TGCTCGTTCA GTGAGCGTCT GCTGCGTCAC
    ATTGGCTTCG TGAGCTTTAT TGATCTACAG CCATTTGCCA TCATCGAATG GCTAGTGCCT
    GAGGTGTTGG TTTTCGCCAC CTCCCTGGGT TCGTATCTCA CGGTGAAGCG AGTGGCCTCA
    CAGCCCGTCG GCGCCGAGCA GCTGGAGAAC GGCGAGGTGG TCGATGGCCA GGCGGAAAAC
    GCGCAGACAT CTTCTCAGCC ATCTGCCGCA GATGCCAATG GAGGAGATGT GCAACAGGCC
    ACGGTCACCA CGCCGCTGCA GCAACAGCAG CAGCAGCTGA GGAAACGAGT GTCCATGATC
    AGCCAGCACA TACACTTTGA GGGCTTGGTC AAGATCTCTC CTCTGTTCTG CCTGGCCACG
    CTGTTCTTTG CGGCCGTACT GCGTCCCTCG GTGCCTGGCG GATTTTACTT TCTCATTTTC
    CTGCTGTCCG GCACCTACTG GGCAACATGC CAGACGCTGC AACGGGGCTT CGCTTTGTTG
    CTGCGCTGCG TGATGGTCGT CCTCGTGCTG CACTCCCTGT CCATTGTGTC ATACCAGACG
    CCATGGATGC AGAGCCACCT CAATCATACC ACCCTGACAG CTCGATTGAT TGGACTGGAA
    CCGCTTATTG AATCCTACTG CTCGCCGGAT ATACGAGTCT TTCTGTACAA TAATAAGCTA
    TCCCTGGACT CGTACCTCAA TCCCTTTGCG TTGTTCTTTG CCTATTTCGC ACTGGCACTG
    ACCACCAAGC ATCTGATTAA GCCACGGGTT TCTTTGCGCC GAGATCAGCG TGCAACGTTA
    AATGAACCGA CTGAGACGAC GCCTTTGGTG CGGCAAAGCA CGCGAAAGGC ACGTACACCC
    CAACCGCTGG AAAGTGGATC CTCGGTGGCG CCAAGTGTCA CTCAGCGGGG CAACGACATG
    CAGCTGGAAT CGATGGAGCA GCGATCGGAA CAGGAGAACA CCACCACATC CATACTGGAT
    CAAATATCGT ATGGATTCGT CAGCGTGGGA GGATTCATAT ATCAGAATAG CTATATATTC
    ACCAATATTC TTATGATGGC CTGGTCCATA GTGTATCACA GTTGGCTGAC TTTTGTCCTC
    TTGCTGTCGG CCAACGTACT GTGGATGATT CCCAACCAGA GGAAGGCCAT GATGCGGTCC
    AGTCCCTTTA TAGTTCTGTA TGCTGAGGCC CTTTTGATTG CCCAATACAT ATACGGCATG
    GATCTCAACA ACGAAGAGCT GCCCACGAGC GTTCCCACTG CGGGCATTAA CCTGCAACAA
    ATTGGCTTCG AACGACCCAT CGAGAACCAA ATGCGGCCAT GTGTGCCGCT GATCGTGAAG
    ACAGCGTTTG TGCTAATGTT TTGGGTGACA TCACGACAGT TCTTCAAGGA GAAGCGCGAT
    CGCCGACGGG ACAGCACTCT GGCGGACTTC ATTGCCCCAC TGCAGATTAC TGTGGGATCG
    GCTGGCTCCA GCTACCTCAT CAACGATGGC AAGAAGACCT CAAAGTTCCT AAAGAAGGCC
    GGCGATGTGA TCAAAAATCT ATTGGTGCGT CTGTGGATCT GGCTACTCGT GCTGGTTATC
    TTCCTTTGCG CCATCACTGG CGAGAACATG ACCGGCTTCC GCATCTGCTA CATGGCCCTG
    TTTCTATTCT TCTTGCTAGT CTTTCAATCT TCGTCCAAGG CGTGGGTTAA GATCATGTAC
    GGCTTCTGGC TGTTTCTGAT CTTCTATGCC ATGTCCATAC TTATATTGAT CTACACATAT
    CAATTCGACA AGTTCGACAC GTACTGGAGC GACTATCTTA ATGTGTCCGC GACTTTGCAA
    AAGGACATCG GCCTTAAGCG CTATCAGACC AAGGATCTGT TCCTCCATTT GGTCTCACCG
    ACGATAATTG TGATCCTGAC CGTCATACAA GTGCACTACT TCCACAAGCG CTTTATTGCA
    TCATTGCAGC AGCAGCCGTT GGCTGGCGGA TCGGCACAGC AGAAACCCAC GGAGACAACT
    GCCTTGGAAC CGGCGCCATC GAAGCGACGT GGCAGCGCCG GTTCACTGCG TAAATCTCAG
    GGTCCATCGG CGGAGGCTGC TCCAGGAGCC ACCACCGATT TCGAGACATC TGTGCGAGAC
    TTGGTGCGCA TATCGTTCCG CAAGATCAAG AACAAGTCGG AGTACATCTT CAAGAACTTC
    AAGGATGTCT TCTGGCGCTT CCTGGAGCTG CACATCATGA AGGCTGTGTA TATCGCAGCC
    TTCGTGTGCA GTGTCAGCGA AGTCTGCGTA CTGCACATTA TCTTTGTGGG TTTCTGTGTG
    CTGGGCGCCA CCTCGCGGAA GGCCGTTCAG GTGGTGATCA GCCGCCTCAT CTCGTTCATT
    GTCACCGTCA TAGTTCTGTC CAAGATGATC TACCAGATCG AGTACTTGAG TCACTCGCAG
    CACAATGTGG TTTGTTCTGA CAACCGGACT GCCAACAATG CGGAGTGGAT TGGTCTCACC
    AAGGCTGACA AGGTAACTGG CGGACTGATG AGCCTGTTGC GCACCTACAT CATCTACATG
    GTTATTGTGA CCATGCACGC AGTGATCAGT TTGCGGCAGC TTCAAATGCG CGTCAAGATC
    GGAGCACTGA ATGCTCCACC CACCAAGCTG CTGTTCCCCA ATATTATTCG AGCTGATGCT
    GAGAAGGATC TGGTGGGACT GGTCAAGTAT CTCCTCAACT TTGGCTTCTA CAAATTCGGC
    ATTGAGATAT CGCTAATCGC GCTGGTCTCT ACCATCACAT ATCGCCAGGA TATTGTTGCC
    GTAGTCTATG CTCTGTGGCT AGTGGTGCTT TTGCTTCTAC GGAGATCGCA GTGCGCCAAA
    ATATGGGGCG TGTTTCAGGC ATTCTTTGCC ATCTCCATAC TGACACAGTA TATAGTGCTG
    GTAGGATTGC CGCCGAGCTC ATGCCTGGTG TTTCCCTGGG ATGAAGGTCC CTTCGGCGAG
    GGCATACAAC GCTGGGCGAT GCTGCCAGGA GCCCTGCACT TCAACCACGT ACCCAAGCTG
    ATCTTCGACT TTATTGTCTT GGTCATTCTG AATCGACAGA AGAGTATCTT CTGCATCGAA
    CAGCGTTATG CCAGTAACGA TGACTACCCG GGTGGCAGCA ATCGCAGTGT GATCGCGGAT
    ATTGCTCAGC TAGGTCGCGT TCCCTTCGAC AATCCCACCC ACGACTTTTG TTCGTACATA
    CGCAACTACT CGGACATCCT TAAGAACGGA GTGCTGTGCG GCTTCTACTG GTTTACTCTG
    GCAGTTGTTT TCTTGGCCGG CACCAATATT GCTGATCTGC TGGCACTGGG TTATCTGATC
    GGAGCGTTTA TCTTCCTGTG GCAGGGATCG GATTTCTATC TGCGTCCCAT ACACACCATC
    ATCTTTCGCT GGAAGTGGCT GCTGGCATTC AATGTGGCCA ACATACTTAT CAAGACGTCC
    TTCCAAATGG CCGGCTGTTT GTTCATGACC CAACTGACGA AAGACTGCTG CTGGCTGGTG
    CACATGCTCG GCATCACATG TACGAGCAAT GTACTTACAG AGCAAATAAT GCTGCCCGAG
    GAGGCTGAAT TGGCGCTTAA GCCAGGCGAA TGTCCTAAGA TCACCCACCA GGTGGTCCTT
    CTGTGGGACA CGATTTGCTT TGCCTTCATC ATCTTCCAGC TGCGCATCTT CAAGTCGCAC
    TACTTCTGTC ACATCATAAC GGACACCAAA GCAAATAACA TCCTGGCCTC AAGAGGAGCC
    GACATCATTG AGAGCCTACG ACATAAGCAG ATTGCACATC GACACGACCA TGAAAAGCAG
    GTGCTGCATA AGATCAAGCG AAAGATGGAG CGCATCCGTG CCACGCAGCA GAAGATGCTA
    CGACCCTTGG ACAAACAAAC CCACTTCGAC GAACATGGTT ATCCACTTCC TGCACCAACA
    GTACGCAGAA GGAAGGAAAT TAAATTACAT CCACATGCTA CCCGTGCTGG TGACTACTAT
    ATGTTCGAGG AGATGGACGA TAAGTTTGAG CTCGACTTGA TACACGACGA GATCGACTTC
    CTCGAGGAGG AGAACATCAC CGAGAGCGAG ATGAAGATGC AGCGACGCAA GACCCTCTAC
    GATAAGTCGA AGGATGCACC CACAGGCGAG TTTCCCTCCA CCAGTAAGGG TATTTCGAAG
    GAGCGCGATG CGGCAACGGC TTCCAGTTCG GCTAGTCCAG CGCCCACTAG GGATGTGGGT
    GATCTGCCTG TGATTCCACC ACCCTCCACT GGCCTGGGAC GTGAGCAAAC CTCCAAGGAG
    ACCTCCGATA GCAAGTCCAA AATGGAAGTG GACAGCGGAG AGGTGACGGC CAAGGATTCG
    GATGAGGACT TCGATACCAA TCCCATTATC AGACTGCTCG AGGGCTTCTT GGTCACGCTG
    ACCATAAGAC TGAACCGCTT TTCGCGCAAC TATCGCTTTG TGAATCGCAT CCTGGCCGGC
    GAAAAGAAGA CCCTAAAGGA GTCCAGTTCG TTGAACCGTC TGGGGCTGTC CAGTGCCGCT
    GCCATGTTCC ACTTCCTCAA ATCGAATTTA GAGAGCGATG AAAGTGAACC GCCCGCCTCC
    TCATCCACCC CGCGGCGGGT GGTGATCGCA CCACCGAATG CCACCGAGCA CTCAGACCCC
    ACCAGCACCA CACTGAACAC GAACACGACA ACCACACCGC TATCACCACC AGAACCACTG
    CAACCACTGC AACCACTGCA ACCAAATACA ACCAGTACAC CACAGCAACA GCATCAGCAT
    ATTCGCGCTG CCGAAGAAAT CATCGAACTG CCTGTAGATA CCGTGGATGG AGTCGCCCAT
    AGAAAACAAT CAATCAATTC ATCGCCGCCA GCCAAGGGCG CGGGTGAATT CAATCTGGAG
    GAGGAGAACT TCGCCCAGAG AGATCATCAC ATCATTGTCG AGGTGTTGAT CTCCTCGTGG
    TATGCCCTGT TGGCCAACAC GGATCTCATC TGCTACATTG TGGTTTTCAT CAATCAGGTG
    GTCAATGCCA GTCTCATTTC GCTGCCGCTG CCCATTATGG TCTTTTTGTG GGGCACATTG
    TCTCTGCCGC GTCCCACTAA AACCTTCTGG GTCACGCTGA TTGCCTACAC CCAGGCCATC
    GTGCTGATCA AGTGCATCTT CCAGTTTAAG CTGATCTGGT CCAATTACCA CCAACTGCCC
    AATCAGCCGC TAACACCGGC CAAAATATTC GGCGTTGAGA ATAAGGCCCA TTATGCGATT
    TACGACCTGA TACTATTGCT GGTTCTATTC CTGCATCGCT ATTTGCTTAA ATCACAAGGC
    CTGTGGAAAT CGGGCTACAA GGACACGGAT AACCAGTTCA CCAAGCCCAC CGCTAGCATT
    GATGAACGCG ACGATAGCGA CAACTTATCC CAACCGGATT CCCGCCAGCT AAACGATGAT
    GCTGCTCAGA AGCTGAGTCT CCAAGTGAGC CAGGCTTCCT TGCCAGGATC GCCCGAGTTT
    AGCAAGACAG GCATCAATCA GCTAGAGCGC ACCAAGTACA CTTCATCGCT GTACAAATTC
    TTCTTCAGTT TGGTCCACAA ATCCCGTCTA GCCACAGATG TTTACGCTCT GATGTTCCTC
    TGCGATTTTG TGAACTTCTT CGTGCTTCTT TTCGGCTTCA CTGCTTTTGG AACTCAGCAA
    ACGGAAAGTG ATGAAGGTGT GCAGACATAT CTTGCGGAGA ACAAAGTGCC CATACCATTC
    CTGATTATGC TACTTGTTCA GTTCCTGCTC ATTGTCATTG ATCGAGCTTT GTATCTGCGC
    AAAGCCCTGG TGAACAAGAT CATCTTCCAC TTCTTTTCGG TTATCGGAAT ACACATCTGG
    ATGTTCTTCG TTGTGCCTGC AGTTACGGAG CGCACTTTCA ACTCCCTCGC ACCTCCAATA
    ATATTCTACG TTATCAAGTG CTTTTACATG CTGCTCAGCT CTTATCAAAT CAAATCCGGT
    TACCCTAAGC GCATTCTAGG CAACTTCTTC ACCAAGGGTT TCTCGATGGT CAATATGATT
    GCCTTTAAGG TGTACATGCA GATTCCATTC CTGTACGAGC TGCGAACCAT ATTAGATTGG
    GTGTGCATTG ACAGCACCAT GACCATTTTC GATTGGCTAA AGATGGAGGA CATTTTCTCG
    AATATATATC TTATCCGCTG CACCCGGCAG TCAGAGACTG ATTTTCCGGC CATGAGAGCT
    CAGAAGAAGG CCTCACTTTC AAAGCTGATA ATGGGTGGCA CCATCGTCCT GCTGATAGTG
    ATTTGCATTT GGGGACCATT GTGTTTGTTT GCACTTGGCA ACGCGGTGGG CACCTCGAAT
    GTGCCGTTCC ATGTGTCGCT ATCAATACGA ATTGGACCCT ATGATCCCAT CTACACAACG
    AACAACTACG ACAGTATCTT CGAGATCAAT CCTGAGATGT ACTCTCAGAT GACTAATGCA
    TACATAAAGG AGAAACAGGC TTTGACGTTT ATTGCCGGCT ATGATGCAAC GGATGTAGCG
    GCGGTTAGAC TAGCTGGCAA CTCGCCATCT CTGTGGAACA TAGCACCGCC GGATAGGCAG
    CGACTACTAA ATGATTTAAG AAACAATCAC ACGCTGAAGG CCCGGTTCTC TTATTCCCTC
    ACCCGAAAGG CTCCTGCCAA GGGGCTAAAG GAAAATGTGG GCGACGAGCA TGCCATATCG
    CTGGACGAGT CCTTTGAGGG ACGAGCAGCA CTCATTCACA TGCTAAGCGA AACCCATGAT
    GTGGAGCCCA TTTACAGCAA TGGCACCACA AATGGAACCA CTCCCGAAGT TGAAGAAGTG
    GTGGTGATAC CCGGAATGAT ACCCAAGTTT ATCAAGGTTC TCAATTCGGG CGACGCTGCG
    GTGGTAAGTG TTTTGAGCCC AAAACACTAC GACTACCGAC CGTTGGTTAT CAAAATGCAT
    CGGGACAACG AGACCAATGG GTTGTGGTGG GAGATTCGGG ACTATTGCAA CGACACCTTC
    TACAACGAAA CTCTATCGAA GTTTGCATAC AGCAACTGCA CTTCGGGAAT TGTGATGTAC
    ACGTTCAACG ATAAAAAGTT TCCATCCACG TTCAGTTTCC TCACAGCGGG AGGCATCATT
    GGTCTGTACA CTACCTTTGT GTTATTGGCC TCGCGCTTTA TGAAGTCCTT CATTGGCGGG
    CAAAACAGAA AGATCATGTT TGAGGATCTG CCCTATGTTG ATAGAGTGCT GCAGCTCTGT
    CTGGACATTT ATCTGGTACG GGAGGCATTG GAATTCGCTT TGGAAGAGGA CCTGTTTGCC
    AAATTACTCT TCCTATACCG ATCGCCCGAA ACGCTAATCA AGTGGACGCG TCCCAAGGAG
    GAGTACGTGG ACGATGATGG CGACACCGAC TCGATTCCCA GTCGAATGAG CGTGCGCCGG
    CCGGAGCAAC TGCAGCCACA GCAACCGCAA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa15942
    Clone ID Related Accession (Same CDS sequence) NM_001201790.2
    Accession Version NM_001201790.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7656bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product piezo, isoform I
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001201790.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGTCTTCA GCTATGCGTG CATGGTGCTC CAGCGCATCG TGGTGCCAGC GGTTCTGGTA 
    CTCGCTGCCC TGATGCGACC AGTGGGCATA TCCTTTGTCT ACCTTTTGAT GTTCTTTGTG
    TCGCCCTTTG TTCCGCTGGC CACGCGACGC AACTTCAAAG GATCTGTGAC TGCCTTCTTC
    ATCATCCTGC TGACGTTGAG CACGCTGGTC CTCTTGGGTC ACATAACGCT ACAGATTCTG
    GCGGTCAGCC TCACGCTGCC CATCTACAAT TGCTCGTTCA GTGAGCGTCT GCTGCGTCAC
    ATTGGCTTCG TGAGCTTTAT TGATCTACAG CCATTTGCCA TCATCGAATG GCTAGTGCCT
    GAGGTGTTGG TTTTCGCCAC CTCCCTGGGT TCGTATCTCA CGGTGAAGCG AGTGGCCTCA
    CAGCCCGTCG GCGCCGAGCA GCTGGAGAAC GGCGAGGTGG TCGATGGCCA GGCGGAAAAC
    GCGCAGACAT CTTCTCAGCC ATCTGCCGCA GATGCCAATG GAGGAGATGT GCAACAGGCC
    ACGGTCACCA CGCCGCTGCA GCAACAGCAG CAGCAGCTGA GGAAACGAGT GTCCATGATC
    AGCCAGCACA TACACTTTGA GGGCTTGGTC AAGATCTCTC CTCTGTTCTG CCTGGCCACG
    CTGTTCTTTG CGGCCGTACT GCGTCCCTCG GTGCCTGGCG GATTTTACTT TCTCATTTTC
    CTGCTGTCCG GCACCTACTG GGCAACATGC CAGACGCTGC AACGGGGCTT CGCTTTGTTG
    CTGCGCTGCG TGATGGTCGT CCTCGTGCTG CACTCCCTGT CCATTGTGTC ATACCAGACG
    CCATGGATGC AGAGCCACCT CAATCATACC ACCCTGACAG CTCGATTGAT TGGACTGGAA
    CCGCTTATTG AATCCTACTG CTCGCCGGAT ATACGAGTCT TTCTGTACAA TAATAAGCTA
    TCCCTGGACT CGTACCTCAA TCCCTTTGCG TTGTTCTTTG CCTATTTCGC ACTGGCACTG
    ACCACCAAGC ATCTGATTAA GCCACGGTTG GTGCGGCAAA GCACGCGAAA GGCACGTACA
    CCCCAACCGC TGGAAAGTGG ATCCTCGGTG GCGCCAAGTG TCACTCAGCG GGGCAACGAC
    ATGCAGCTGG AATCGATGGA GCAGCGATCG GAACAGGAGA ACACCACCAC ATCCATACTG
    GATCAAATAT CGTATGGATT CGTCAGCGTG GGAGGATTCA TATATCAGAA TAGCTATATA
    TTCACCAATA TTCTTATGAT GGCCTGGTCC ATAGTGTATC ACAGTTGGCT GACTTTTGTC
    CTCTTGCTGT CGGCCAACGT ACTGTGGATG ATTCCCAACC AGAGGAAGGC CATGATGCGG
    TCCAGTCCCT TTATAGTTCT GTATGCTGAG GCCCTTTTGA TTGCCCAATA CATATACGGC
    ATGGATCTCA ACAACGAAGA GCTGCCCACG AGCGTTCCCA CTGCGGGCAT TAACCTGCAA
    CAAATTGGCT TCGAACGACC CATCGAGAAC CAAATGCGGC CATGTGTGCC GCTGATCGTG
    AAGACAGCGT TTGTGCTAAT GTTTTGGGTG ACATCACGAC AGTTCTTCAA GGAGAAGCGC
    GATCGCCGAC GGGACAGCAC TCTGGCGGAC TTCATTGCCC CACTGCAGAT TACTGTGGGA
    TCGGCTGGCT CCAGCTACCT CATCAACGAT GGCAAGAAGA CCTCAAAGTT CCTAAAGAAG
    GCCGGCGATG TGATCAAAAA TCTATTGGTG CGTCTGTGGA TCTGGCTACT CGTGCTGGTT
    ATCTTCCTTT GCGCCATCAC TGGCGAGAAC ATGACCGGCT TCCGCATCTG CTACATGGCC
    CTGTTTCTAT TCTTCTTGCT AGTCTTTCAA TCTTCGTCCA AGGCGTGGGT TAAGATCATG
    TACGGCTTCT GGCTGTTTCT GATCTTCTAT GCCATGTCCA TACTTATATT GATCTACACA
    TATCAATTCG ACAAGTTCGA CACGTACTGG AGCGACTATC TTAATGTGTC CGCGACTTTG
    CAAAAGGACA TCGGCCTTAA GCGCTATCAG ACCAAGGATC TGTTCCTCCA TTTGGTCTCA
    CCGACGATAA TTGTGATCCT GACCGTCATA CAAGTGCACT ACTTCCACAA GCGCTTTATT
    GCATCATTGC AGCAGCAGCC GTTGGCTGGC GGATCGGCAC AGCAGAAACC CACGGAGACA
    ACTGCCTTGG AACCGGCGCC ATCGAAGCGA CGTGGCAGCG CCGGTTCACT GCGTAAATCT
    CAGGGTCCAT CGGCGGAGGC TGCTCCAGGA GCCACCACCG ATTTCGAGAC ATCTGTGCGA
    GACTTGGTGC GCATATCGTT CCGCAAGATC AAGAACAAGT CGGAGTACAT CTTCAAGAAC
    TTCAAGGATG TCTTCTGGCG CTTCCTGGAG CTGCACATCA TGAAGGCTGT GTATATCGCA
    GCCTTCGTGT GCAGTGTCAG CGAAGTCTGC GTACTGCACA TTATCTTTGT GGGTTTCTGT
    GTGCTGGGCG CCACCTCGCG GAAGGCCGTT CAGGTGGTGA TCAGCCGCCT CATCTCGTTC
    ATTGTCACCG TCATAGTTCT GTCCAAGATG ATCTACCAGA TCGAGTACTT GAGTCACTCG
    CAGCACAATG TGGTTTGTTC TGACAACCGG ACTGCCAACA ATGCGGAGTG GATTGGTCTC
    ACCAAGGCTG ACAAGGTAAC TGGCGGACTG ATGAGCCTGT TGCGCACCTA CATCATCTAC
    ATGGTTATTG TGACCATGCA CGCAGTGATC AGTTTGCGGC AGCTTCAAAT GCGCGTCAAG
    ATCGGAGCAC TGAATGCTCC ACCCACCAAG CTGCTGTTCC CCAATATTAT TCGAGCTGAT
    GCTGAGAAGG ATCTGGTGGG ACTGGTCAAG TATCTCCTCA ACTTTGGCTT CTACAAATTC
    GGCATTGAGA TATCGCTAAT CGCGCTGGTC TCTACCATCA CATATCGCCA GGATATTGTT
    GCCGTAGTCT ATGCTCTGTG GCTAGTGGTG CTTTTGCTTC TACGGAGATC GCAGTGCGCC
    AAAATATGGG GCGTGTTTCA GGCATTCTTT GCCATCTCCA TACTGACACA GTATATAGTG
    CTGGTAGGAT TGCCGCCGAG CTCATGCCTG GTGTTTCCCT GGGATGAAGG TCCCTTCGGC
    GAGGGCATAC AACGCTGGGC GATGCTGCCA GGAGCCCTGC ACTTCAACCA CGTACCCAAG
    CTGATCTTCG ACTTTATTGT CTTGGTCATT CTGAATCGAC AGAAGAGTAT CTTCTGCATC
    GAACAGCGTT ATGCCAGTAA CGATGACTAC CCGGGTGGCA GCAATCGCAG TGTGATCGCG
    GATATTGCTC AGCTAGGTCG CGTTCCCTTC GACAATCCCA CCCACGACTT TTGTTCGTAC
    ATACGCAACT ACTCGGACAT CCTTAAGAAC GGAGTGCTGT GCGGCTTCTA CTGGTTTACT
    CTGGCAGTTG TTTTCTTGGC CGGCACCAAT ATTGCTGATC TGCTGGCACT GGGTTATCTG
    ATCGGAGCGT TTATCTTCCT GTGGCAGGGA TCGGATTTCT ATCTGCGTCC CATACACACC
    ATCATCTTTC GCTGGAAGTG GCTGCTGGCA TTCAATGTGG CCAACATACT TATCAAGACG
    TCCTTCCAAA TGGCCGGCTG TTTGTTCATG ACCCAACTGA CGAAAGACTG CTGCTGGCTG
    GTGCACATGC TCGGCATCAC ATGTACGAGC AATGTACTTA CAGAGCAAAT AATGCTGCCC
    GAGGAGGCTG AATTGGCGCT TAAGCCAGGC GAATGTCCTA AGATCACCCA CCAGGTGGTC
    CTTCTGTGGG ACACGATTTG CTTTGCCTTC ATCATCTTCC AGCTGCGCAT CTTCAAGTCG
    CACTACTTCT GTCACATCAT AACGGACACC AAAGCAAATA ACATCCTGGC CTCAAGAGGA
    GCCGACATCA TTGAGAGCCT ACGACATAAG CAGATTGCAC ATCGACACGA CCATGAAAAG
    CAGGTGCTGC ATAAGATCAA GCGAAAGATG GAGCGCATCC GTGCCACGCA GCAGAAGATG
    CTACGACCCT TGGACAAACA AACCCACTTC GACGAACATG GTTATCCACT TCCTGCACCA
    ACAGTACGCA GAAGGAAGGA AATTAAATTA CATCCACATG CTACCCGTGC TGGTGACTAC
    TATATGTTCG AGGAGATGGA CGATAAGTTT GAGCTCGACT TGATACACGA CGAGATCGAC
    TTCCTCGAGG AGGAGAACAT CACCGAGAGC GAGATGAAGA TGCAGCGACG CAAGACCCTC
    TACGATAAGT CGAAGGATGC ACCCACAGGC GAGTTTCCCT CCACCAGTAA GGGTATTTCG
    AAGGAGCGCG ATGCGGCAAC GGCTTCCAGT TCGGCTAGTC CAGCGCCCAC TAGGGATGTG
    GGTGATCTGC CTGTGATTCC ACCACCCTCC ACTGGCCTGG GACGTGAGCA AACCTCCAAG
    GAGACCTCCG ATAGCAAGTC CAAAATGGAA GTGGACAGCG GAGAGGTGAC GGCCAAGGAT
    TCGGATGAGG ACTTCGATAC CAATCCCATT ATCAGACTGC TCGAGGGCTT CTTGGTCACG
    CTGACCATAA GACTGAACCG CTTTTCGCGC AACTATCGCT TTGTGAATCG CATCCTGGCC
    GGCGAAAAGA AGACCCTAAA GGAGTCCAGT TCGTTGAACC GTCTGGGGCT GTCCAGTGCC
    GCTGCCATGT TCCACTTCCT CAAATCGAAT TTAGAGAGCG ATGAAAGTGA ACCGCCCGCC
    TCCTCATCCA CCCCGCGGCG GGTGGTGATC GCACCACCGA ATGCCACCGA GCACTCAGAC
    CCCACCAGCA CCACACTGAA CACGAACACG ACAACCACAC CGCTATCACC ACCAGAACCA
    CTGCAACCAC TGCAACCACT GCAACCAAAT ACAACCAGTA CACCACAGCA ACAGCATCAG
    CATATTCGCG CTGCCGAAGA AATCATCGAA CTGCCTGTAG ATACCGTGGA TGGAGTCGCC
    CATAGAAAAC AATCAATCAA TTCATCGCCG CCAGCCAAGG GCGCGGGTGA ATTCAATCTG
    GAGGAGGAGA ACTTCGCCCA GAGAGATCAT CACATCATTG TCGAGGTGTT GATCTCCTCG
    TGGTATGCCC TGTTGGCCAA CACGGATCTC ATCTGCTACA TTGTGGTTTT CATCAATCAG
    GTGGTCAATG CCAGTCTCAT TTCGCTGCCG CTGCCCATTA TGGTCTTTTT GTGGGGCACA
    TTGTCTCTGC CGCGTCCCAC TAAAACCTTC TGGGTCACGC TGATTGCCTA CACCCAGGCC
    ATCGTGCTGA TCAAGTGCAT CTTCCAGTTT AAGCTGATCT GGTCCAATTA CCACCAACTG
    CCCAATCAGC CGCTAACACC GGCCAAAATA TTCGGCGTTG AGAATAAGGC CCATTATGCG
    ATTTACGACC TGATACTATT GCTGGTTCTA TTCCTGCATC GCTATTTGCT TAAATCACAA
    GGCCTGTGGA AATCGGGCTA CAAGGACACG GATAACCAGT TCACCAAGCC CACCGCTAGC
    ATTGATGAAC GCGACGATAG CGACAACTTA TCCCAACCGG ATTCCCGCCA GCTAAACGAT
    GATGCTGCTC AGAAGCTGAG TCTCCAAGTG AGCCAGGCTT CCTTGCCAGG ATCGCCCGAG
    TTTAGCAAGA CAGGCATCAA TCAGCTAGAG CGCACCAAGT ACACTTCATC GCTGTACAAA
    TTCTTCTTCA GTTTGGTCCA CAAATCCCGT CTAGCCACAG ATGTTTACGC TCTGATGTTC
    CTCTGCGATT TTGTGAACTT CTTCGTGCTT CTTTTCGGCT TCACTGCTTT TGGAACTCAG
    CAAACGGAAA GTGATGAAGG TGTGCAGACA TATCTTGCGG AGAACAAAGT GCCCATACCA
    TTCCTGATTA TGCTACTTGT TCAGTTCCTG CTCATTGTCA TTGATCGAGC TTTGTATCTG
    CGCAAAGCCC TGGTGAACAA GATCATCTTC CACTTCTTTT CGGTTATCGG AATACACATC
    TGGATGTTCT TCGTTGTGCC TGCAGTTACG GAGCGCACTT TCAACTCCCT CGCACCTCCA
    ATAATATTCT ACGTTATCAA GTGCTTTTAC ATGCTGCTCA GCTCTTATCA AATCAAATCC
    GGTTACCCTA AGCGCATTCT AGGCAACTTC TTCACCAAGG GTTTCTCGAT GGTCAATATG
    ATTGCCTTTA AGGTGTACAT GCAGATTCCA TTCCTGTACG AGCTGCGAAC CATATTAGAT
    TGGGTGTGCA TTGACAGCAC CATGACCATT TTCGATTGGC TAAAGATGGA GGACATTTTC
    TCGAATATAT ATCTTATCCG CTGCACCCGG CAGTCAGAGA CTGATTTTCC GGCCATGAGA
    GCTCAGAAGA AGGCCTCACT TTCAAAGCTG ATAATGGGTG GCACCATCGT CCTGCTGATA
    GTGATTTGCA TTTGGGGACC ATTGTGTTTG TTTGCACTTG GCAACGCGGT GGGCACCTCG
    AATGTGCCGT TCCATGTGTC GCTATCAATA CGAATTGGAC CCTATGATCC CATCTACACA
    ACGAACAACT ACGACAGTAT CTTCGAGATC AATCCTGAGA TGTACTCTCA GATGACTAAT
    GCATACATAA AGGAGAAACA GGCTTTGACG TTTATTGCCG GCTATGATGC AACGGATGTA
    GCGGCGGTTA GACTAGCTGG CAACTCGCCA TCTCTGTGGA ACATAGCACC GCCGGATAGG
    CAGCGACTAC TAAATGATTT AAGAAACAAT CACACGCTGA AGGCCCGGTT CTCTTATTCC
    CTCACCCGAA AGGCTCCTGC CAAGGGGCTA AAGGAAAATG TGGGCGACGA GCATGCCATA
    TCGCTGGACG AGTCCTTTGA GGGACGAGCA GCACTCATTC ACATGCTAAG CGAAACCCAT
    GATGTGGAGC CCATTTACAG CAATGGCACC ACAAATGGAA CCACTCCCGA AGTTGAAGAA
    GTGGTGGTGA TACCCGGAAT GATACCCAAG TTTATCAAGG TTCTCAATTC GGGCGACGCT
    GCGGTGGTAA GTGTTTTGAG CCCAAAACAC TACGACTACC GACCGTTGGT TATCAAAATG
    CATCGGGACA ACGAGACCAA TGGGTTGTGG TGGGAGATTC GGGACTATTG CAACGACACC
    TTCTACAACG AAACTCTATC GAAGTTTGCA TACAGCAACT GCACTTCGGG AATTGTGATG
    TACACGTTCA ACGATAAAAA GTTTCCATCC ACGTTCAGTT TCCTCACAGC GGGAGGCATC
    ATTGGTCTGT ACACTACCTT TGTGTTATTG GCCTCGCGCT TTATGAAGTC CTTCATTGGC
    GGGCAAAACA GAAAGATCAT GTTTGAGGAT CTGCCCTATG TTGATAGAGT GCTGCAGCTC
    TGTCTGGACA TTTATCTGGT ACGGGAGGCA TTGGAATTCG CTTTGGAAGA GGACCTGTTT
    GCCAAATTAC TCTTCCTATA CCGATCGCCC GAAACGCTAA TCAAGTGGAC GCGTCCCAAG
    GAGGAGTACG TGGACGATGA TGGCGACACC GACTCGATTC CCAGTCGAAT GAGCGTGCGC
    CGGCCGGAGC AACTGCAGCC ACAGCAACCG CAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001188719.1
    CDS577..8232
    Translation

    Target ORF information:

    RefSeq Version NM_001201790.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster piezo, transcript variant I (Piezo), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001201790.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGTCTTCA GCTATGCGTG CATGGTGCTC CAGCGCATCG TGGTGCCAGC GGTTCTGGTA 
    CTCGCTGCCC TGATGCGACC AGTGGGCATA TCCTTTGTCT ACCTTTTGAT GTTCTTTGTG
    TCGCCCTTTG TTCCGCTGGC CACGCGACGC AACTTCAAAG GATCTGTGAC TGCCTTCTTC
    ATCATCCTGC TGACGTTGAG CACGCTGGTC CTCTTGGGTC ACATAACGCT ACAGATTCTG
    GCGGTCAGCC TCACGCTGCC CATCTACAAT TGCTCGTTCA GTGAGCGTCT GCTGCGTCAC
    ATTGGCTTCG TGAGCTTTAT TGATCTACAG CCATTTGCCA TCATCGAATG GCTAGTGCCT
    GAGGTGTTGG TTTTCGCCAC CTCCCTGGGT TCGTATCTCA CGGTGAAGCG AGTGGCCTCA
    CAGCCCGTCG GCGCCGAGCA GCTGGAGAAC GGCGAGGTGG TCGATGGCCA GGCGGAAAAC
    GCGCAGACAT CTTCTCAGCC ATCTGCCGCA GATGCCAATG GAGGAGATGT GCAACAGGCC
    ACGGTCACCA CGCCGCTGCA GCAACAGCAG CAGCAGCTGA GGAAACGAGT GTCCATGATC
    AGCCAGCACA TACACTTTGA GGGCTTGGTC AAGATCTCTC CTCTGTTCTG CCTGGCCACG
    CTGTTCTTTG CGGCCGTACT GCGTCCCTCG GTGCCTGGCG GATTTTACTT TCTCATTTTC
    CTGCTGTCCG GCACCTACTG GGCAACATGC CAGACGCTGC AACGGGGCTT CGCTTTGTTG
    CTGCGCTGCG TGATGGTCGT CCTCGTGCTG CACTCCCTGT CCATTGTGTC ATACCAGACG
    CCATGGATGC AGAGCCACCT CAATCATACC ACCCTGACAG CTCGATTGAT TGGACTGGAA
    CCGCTTATTG AATCCTACTG CTCGCCGGAT ATACGAGTCT TTCTGTACAA TAATAAGCTA
    TCCCTGGACT CGTACCTCAA TCCCTTTGCG TTGTTCTTTG CCTATTTCGC ACTGGCACTG
    ACCACCAAGC ATCTGATTAA GCCACGGTTG GTGCGGCAAA GCACGCGAAA GGCACGTACA
    CCCCAACCGC TGGAAAGTGG ATCCTCGGTG GCGCCAAGTG TCACTCAGCG GGGCAACGAC
    ATGCAGCTGG AATCGATGGA GCAGCGATCG GAACAGGAGA ACACCACCAC ATCCATACTG
    GATCAAATAT CGTATGGATT CGTCAGCGTG GGAGGATTCA TATATCAGAA TAGCTATATA
    TTCACCAATA TTCTTATGAT GGCCTGGTCC ATAGTGTATC ACAGTTGGCT GACTTTTGTC
    CTCTTGCTGT CGGCCAACGT ACTGTGGATG ATTCCCAACC AGAGGAAGGC CATGATGCGG
    TCCAGTCCCT TTATAGTTCT GTATGCTGAG GCCCTTTTGA TTGCCCAATA CATATACGGC
    ATGGATCTCA ACAACGAAGA GCTGCCCACG AGCGTTCCCA CTGCGGGCAT TAACCTGCAA
    CAAATTGGCT TCGAACGACC CATCGAGAAC CAAATGCGGC CATGTGTGCC GCTGATCGTG
    AAGACAGCGT TTGTGCTAAT GTTTTGGGTG ACATCACGAC AGTTCTTCAA GGAGAAGCGC
    GATCGCCGAC GGGACAGCAC TCTGGCGGAC TTCATTGCCC CACTGCAGAT TACTGTGGGA
    TCGGCTGGCT CCAGCTACCT CATCAACGAT GGCAAGAAGA CCTCAAAGTT CCTAAAGAAG
    GCCGGCGATG TGATCAAAAA TCTATTGGTG CGTCTGTGGA TCTGGCTACT CGTGCTGGTT
    ATCTTCCTTT GCGCCATCAC TGGCGAGAAC ATGACCGGCT TCCGCATCTG CTACATGGCC
    CTGTTTCTAT TCTTCTTGCT AGTCTTTCAA TCTTCGTCCA AGGCGTGGGT TAAGATCATG
    TACGGCTTCT GGCTGTTTCT GATCTTCTAT GCCATGTCCA TACTTATATT GATCTACACA
    TATCAATTCG ACAAGTTCGA CACGTACTGG AGCGACTATC TTAATGTGTC CGCGACTTTG
    CAAAAGGACA TCGGCCTTAA GCGCTATCAG ACCAAGGATC TGTTCCTCCA TTTGGTCTCA
    CCGACGATAA TTGTGATCCT GACCGTCATA CAAGTGCACT ACTTCCACAA GCGCTTTATT
    GCATCATTGC AGCAGCAGCC GTTGGCTGGC GGATCGGCAC AGCAGAAACC CACGGAGACA
    ACTGCCTTGG AACCGGCGCC ATCGAAGCGA CGTGGCAGCG CCGGTTCACT GCGTAAATCT
    CAGGGTCCAT CGGCGGAGGC TGCTCCAGGA GCCACCACCG ATTTCGAGAC ATCTGTGCGA
    GACTTGGTGC GCATATCGTT CCGCAAGATC AAGAACAAGT CGGAGTACAT CTTCAAGAAC
    TTCAAGGATG TCTTCTGGCG CTTCCTGGAG CTGCACATCA TGAAGGCTGT GTATATCGCA
    GCCTTCGTGT GCAGTGTCAG CGAAGTCTGC GTACTGCACA TTATCTTTGT GGGTTTCTGT
    GTGCTGGGCG CCACCTCGCG GAAGGCCGTT CAGGTGGTGA TCAGCCGCCT CATCTCGTTC
    ATTGTCACCG TCATAGTTCT GTCCAAGATG ATCTACCAGA TCGAGTACTT GAGTCACTCG
    CAGCACAATG TGGTTTGTTC TGACAACCGG ACTGCCAACA ATGCGGAGTG GATTGGTCTC
    ACCAAGGCTG ACAAGGTAAC TGGCGGACTG ATGAGCCTGT TGCGCACCTA CATCATCTAC
    ATGGTTATTG TGACCATGCA CGCAGTGATC AGTTTGCGGC AGCTTCAAAT GCGCGTCAAG
    ATCGGAGCAC TGAATGCTCC ACCCACCAAG CTGCTGTTCC CCAATATTAT TCGAGCTGAT
    GCTGAGAAGG ATCTGGTGGG ACTGGTCAAG TATCTCCTCA ACTTTGGCTT CTACAAATTC
    GGCATTGAGA TATCGCTAAT CGCGCTGGTC TCTACCATCA CATATCGCCA GGATATTGTT
    GCCGTAGTCT ATGCTCTGTG GCTAGTGGTG CTTTTGCTTC TACGGAGATC GCAGTGCGCC
    AAAATATGGG GCGTGTTTCA GGCATTCTTT GCCATCTCCA TACTGACACA GTATATAGTG
    CTGGTAGGAT TGCCGCCGAG CTCATGCCTG GTGTTTCCCT GGGATGAAGG TCCCTTCGGC
    GAGGGCATAC AACGCTGGGC GATGCTGCCA GGAGCCCTGC ACTTCAACCA CGTACCCAAG
    CTGATCTTCG ACTTTATTGT CTTGGTCATT CTGAATCGAC AGAAGAGTAT CTTCTGCATC
    GAACAGCGTT ATGCCAGTAA CGATGACTAC CCGGGTGGCA GCAATCGCAG TGTGATCGCG
    GATATTGCTC AGCTAGGTCG CGTTCCCTTC GACAATCCCA CCCACGACTT TTGTTCGTAC
    ATACGCAACT ACTCGGACAT CCTTAAGAAC GGAGTGCTGT GCGGCTTCTA CTGGTTTACT
    CTGGCAGTTG TTTTCTTGGC CGGCACCAAT ATTGCTGATC TGCTGGCACT GGGTTATCTG
    ATCGGAGCGT TTATCTTCCT GTGGCAGGGA TCGGATTTCT ATCTGCGTCC CATACACACC
    ATCATCTTTC GCTGGAAGTG GCTGCTGGCA TTCAATGTGG CCAACATACT TATCAAGACG
    TCCTTCCAAA TGGCCGGCTG TTTGTTCATG ACCCAACTGA CGAAAGACTG CTGCTGGCTG
    GTGCACATGC TCGGCATCAC ATGTACGAGC AATGTACTTA CAGAGCAAAT AATGCTGCCC
    GAGGAGGCTG AATTGGCGCT TAAGCCAGGC GAATGTCCTA AGATCACCCA CCAGGTGGTC
    CTTCTGTGGG ACACGATTTG CTTTGCCTTC ATCATCTTCC AGCTGCGCAT CTTCAAGTCG
    CACTACTTCT GTCACATCAT AACGGACACC AAAGCAAATA ACATCCTGGC CTCAAGAGGA
    GCCGACATCA TTGAGAGCCT ACGACATAAG CAGATTGCAC ATCGACACGA CCATGAAAAG
    CAGGTGCTGC ATAAGATCAA GCGAAAGATG GAGCGCATCC GTGCCACGCA GCAGAAGATG
    CTACGACCCT TGGACAAACA AACCCACTTC GACGAACATG GTTATCCACT TCCTGCACCA
    ACAGTACGCA GAAGGAAGGA AATTAAATTA CATCCACATG CTACCCGTGC TGGTGACTAC
    TATATGTTCG AGGAGATGGA CGATAAGTTT GAGCTCGACT TGATACACGA CGAGATCGAC
    TTCCTCGAGG AGGAGAACAT CACCGAGAGC GAGATGAAGA TGCAGCGACG CAAGACCCTC
    TACGATAAGT CGAAGGATGC ACCCACAGGC GAGTTTCCCT CCACCAGTAA GGGTATTTCG
    AAGGAGCGCG ATGCGGCAAC GGCTTCCAGT TCGGCTAGTC CAGCGCCCAC TAGGGATGTG
    GGTGATCTGC CTGTGATTCC ACCACCCTCC ACTGGCCTGG GACGTGAGCA AACCTCCAAG
    GAGACCTCCG ATAGCAAGTC CAAAATGGAA GTGGACAGCG GAGAGGTGAC GGCCAAGGAT
    TCGGATGAGG ACTTCGATAC CAATCCCATT ATCAGACTGC TCGAGGGCTT CTTGGTCACG
    CTGACCATAA GACTGAACCG CTTTTCGCGC AACTATCGCT TTGTGAATCG CATCCTGGCC
    GGCGAAAAGA AGACCCTAAA GGAGTCCAGT TCGTTGAACC GTCTGGGGCT GTCCAGTGCC
    GCTGCCATGT TCCACTTCCT CAAATCGAAT TTAGAGAGCG ATGAAAGTGA ACCGCCCGCC
    TCCTCATCCA CCCCGCGGCG GGTGGTGATC GCACCACCGA ATGCCACCGA GCACTCAGAC
    CCCACCAGCA CCACACTGAA CACGAACACG ACAACCACAC CGCTATCACC ACCAGAACCA
    CTGCAACCAC TGCAACCACT GCAACCAAAT ACAACCAGTA CACCACAGCA ACAGCATCAG
    CATATTCGCG CTGCCGAAGA AATCATCGAA CTGCCTGTAG ATACCGTGGA TGGAGTCGCC
    CATAGAAAAC AATCAATCAA TTCATCGCCG CCAGCCAAGG GCGCGGGTGA ATTCAATCTG
    GAGGAGGAGA ACTTCGCCCA GAGAGATCAT CACATCATTG TCGAGGTGTT GATCTCCTCG
    TGGTATGCCC TGTTGGCCAA CACGGATCTC ATCTGCTACA TTGTGGTTTT CATCAATCAG
    GTGGTCAATG CCAGTCTCAT TTCGCTGCCG CTGCCCATTA TGGTCTTTTT GTGGGGCACA
    TTGTCTCTGC CGCGTCCCAC TAAAACCTTC TGGGTCACGC TGATTGCCTA CACCCAGGCC
    ATCGTGCTGA TCAAGTGCAT CTTCCAGTTT AAGCTGATCT GGTCCAATTA CCACCAACTG
    CCCAATCAGC CGCTAACACC GGCCAAAATA TTCGGCGTTG AGAATAAGGC CCATTATGCG
    ATTTACGACC TGATACTATT GCTGGTTCTA TTCCTGCATC GCTATTTGCT TAAATCACAA
    GGCCTGTGGA AATCGGGCTA CAAGGACACG GATAACCAGT TCACCAAGCC CACCGCTAGC
    ATTGATGAAC GCGACGATAG CGACAACTTA TCCCAACCGG ATTCCCGCCA GCTAAACGAT
    GATGCTGCTC AGAAGCTGAG TCTCCAAGTG AGCCAGGCTT CCTTGCCAGG ATCGCCCGAG
    TTTAGCAAGA CAGGCATCAA TCAGCTAGAG CGCACCAAGT ACACTTCATC GCTGTACAAA
    TTCTTCTTCA GTTTGGTCCA CAAATCCCGT CTAGCCACAG ATGTTTACGC TCTGATGTTC
    CTCTGCGATT TTGTGAACTT CTTCGTGCTT CTTTTCGGCT TCACTGCTTT TGGAACTCAG
    CAAACGGAAA GTGATGAAGG TGTGCAGACA TATCTTGCGG AGAACAAAGT GCCCATACCA
    TTCCTGATTA TGCTACTTGT TCAGTTCCTG CTCATTGTCA TTGATCGAGC TTTGTATCTG
    CGCAAAGCCC TGGTGAACAA GATCATCTTC CACTTCTTTT CGGTTATCGG AATACACATC
    TGGATGTTCT TCGTTGTGCC TGCAGTTACG GAGCGCACTT TCAACTCCCT CGCACCTCCA
    ATAATATTCT ACGTTATCAA GTGCTTTTAC ATGCTGCTCA GCTCTTATCA AATCAAATCC
    GGTTACCCTA AGCGCATTCT AGGCAACTTC TTCACCAAGG GTTTCTCGAT GGTCAATATG
    ATTGCCTTTA AGGTGTACAT GCAGATTCCA TTCCTGTACG AGCTGCGAAC CATATTAGAT
    TGGGTGTGCA TTGACAGCAC CATGACCATT TTCGATTGGC TAAAGATGGA GGACATTTTC
    TCGAATATAT ATCTTATCCG CTGCACCCGG CAGTCAGAGA CTGATTTTCC GGCCATGAGA
    GCTCAGAAGA AGGCCTCACT TTCAAAGCTG ATAATGGGTG GCACCATCGT CCTGCTGATA
    GTGATTTGCA TTTGGGGACC ATTGTGTTTG TTTGCACTTG GCAACGCGGT GGGCACCTCG
    AATGTGCCGT TCCATGTGTC GCTATCAATA CGAATTGGAC CCTATGATCC CATCTACACA
    ACGAACAACT ACGACAGTAT CTTCGAGATC AATCCTGAGA TGTACTCTCA GATGACTAAT
    GCATACATAA AGGAGAAACA GGCTTTGACG TTTATTGCCG GCTATGATGC AACGGATGTA
    GCGGCGGTTA GACTAGCTGG CAACTCGCCA TCTCTGTGGA ACATAGCACC GCCGGATAGG
    CAGCGACTAC TAAATGATTT AAGAAACAAT CACACGCTGA AGGCCCGGTT CTCTTATTCC
    CTCACCCGAA AGGCTCCTGC CAAGGGGCTA AAGGAAAATG TGGGCGACGA GCATGCCATA
    TCGCTGGACG AGTCCTTTGA GGGACGAGCA GCACTCATTC ACATGCTAAG CGAAACCCAT
    GATGTGGAGC CCATTTACAG CAATGGCACC ACAAATGGAA CCACTCCCGA AGTTGAAGAA
    GTGGTGGTGA TACCCGGAAT GATACCCAAG TTTATCAAGG TTCTCAATTC GGGCGACGCT
    GCGGTGGTAA GTGTTTTGAG CCCAAAACAC TACGACTACC GACCGTTGGT TATCAAAATG
    CATCGGGACA ACGAGACCAA TGGGTTGTGG TGGGAGATTC GGGACTATTG CAACGACACC
    TTCTACAACG AAACTCTATC GAAGTTTGCA TACAGCAACT GCACTTCGGG AATTGTGATG
    TACACGTTCA ACGATAAAAA GTTTCCATCC ACGTTCAGTT TCCTCACAGC GGGAGGCATC
    ATTGGTCTGT ACACTACCTT TGTGTTATTG GCCTCGCGCT TTATGAAGTC CTTCATTGGC
    GGGCAAAACA GAAAGATCAT GTTTGAGGAT CTGCCCTATG TTGATAGAGT GCTGCAGCTC
    TGTCTGGACA TTTATCTGGT ACGGGAGGCA TTGGAATTCG CTTTGGAAGA GGACCTGTTT
    GCCAAATTAC TCTTCCTATA CCGATCGCCC GAAACGCTAA TCAAGTGGAC GCGTCCCAAG
    GAGGAGTACG TGGACGATGA TGGCGACACC GACTCGATTC CCAGTCGAAT GAGCGTGCGC
    CGGCCGGAGC AACTGCAGCC ACAGCAACCG CAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa15937
    Clone ID Related Accession (Same CDS sequence) NM_001273297.1
    Accession Version NM_001273297.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7716bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product piezo, isoform K
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGTCTTCA GCTATGCGTG CATGGTGCTC CAGCGCATCG TGGTGCCAGC GGTTCTGGTA 
    CTCGCTGCCC TGATGCGACC AGTGGGCATA TCCTTTGTCT ACCTTTTGAT GTTCTTTGTG
    TCGCCCTTTG TTCCGCTGGC CACGCGACGC AACTTCAAAG GATCTGTGAC TGCCTTCTTC
    ATCATCCTGC TGACGTTGAG CACGCTGGTC CTCTTGGGTC ACATAACGCT ACAGATTCTG
    GCGGTCAGCC TCACGCTGCC CATCTACAAT TGCTCGTTCA GTGAGCGTCT GCTGCGTCAC
    ATTGGCTTCG TGAGCTTTAT TGATCTACAG CCATTTGCCA TCATCGAATG GCTAGTGCCT
    GAGGTGTTGG TTTTCGCCAC CTCCCTGGGT TCGTATCTCA CGGTGAAGCG AGTGGCCTCA
    CAGCCCGTCG GCGCCGAGCA GCTGGAGAAC GGCGAGGTGG TCGATGGCCA GGCGGAAAAC
    GCGCAGACAT CTTCTCAGCC ATCTGCCGCA GATGCCAATG GAGGAGATGT GCAACAGGCC
    ACGGTCACCA CGCCGCTGCA GCAACAGCAG CAGCAGCTGA GGAAACGAGT GTCCATGATC
    AGCCAGCACA TACACTTTGA GGGCTTGGTC AAGATCTCTC CTCTGTTCTG CCTGGCCACG
    CTGTTCTTTG CGGCCGTACT GCGTCCCTCG GTGCCTGGCG GATTTTACTT TCTCATTTTC
    CTGCTGTCCG GCACCTACTG GGCAACATGC CAGACGCTGC AACGGGGCTT CGCTTTGTTG
    CTGCGCTGCG TGATGGTCGT CCTCGTGCTG CACTCCCTGT CCATTGTGTC ATACCAGACG
    CCATGGATGC AGAGCCACCT CAATCATACC ACCCTGACAG CTCGATTGAT TGGACTGGAA
    CCGCTTATTG AATCCTACTG CTCGCCGGAT ATACGAGTCT TTCTGTACAA TAATAAGCTA
    TCCCTGGACT CGTACCTCAA TCCCTTTGCG TTGTTCTTTG CCTATTTCGC ACTGGCACTG
    ACCACCAAGC ATCTGATTAA GCCACGGCTG GAGCCCAAGC CCGCCACCGC ACTTGGGCAG
    CAACTAGATT GCAATAGCAG CAGCATCAAC AACACCACCG GCAACAAGGT CAACCGCCAG
    CTATCGCTGC TCACCTCGCA GACATCGCGG GGTCGTCGGG ATGGGTCGAA TCCTGGCGGA
    GGTGGAACGA CCATTACCAC CACCACGACC ACCACCTCCA CCACTGCAAT CCGCAATCAG
    CGCTTGAGTG TTTCTTTGCG CCGAGATCAG CGTGCAACGT TAAATGAACC GACTGAGACG
    ACGCCTTTGG TGCGGCAAAG CACGCGAAAG GCACGTACAC CCCAACCGCT GGAAAGTGGA
    TCCTCGGTGG CGCCAAGTGT CACTCAGCGG GGCAACGACA TGCAGCTGGA ATCGATGGAG
    CAGCGATCGG AACAGGAGAA CACCACCACA TCCATACTGG ATCAAATATC GTATGGATTC
    GTCAGCGTGG GAGGATTCAT ATATCAGAAT AGCTATATAT TCACCAATAT TCTTATGATG
    GCCTGGTCCA TAGTGTATCA CAGTTGGCTG ACTTTTGTCC TCTTGCTGTC GGCCAACGTA
    CTGTGGATGA TTCCCAACCA GAGGAAGGCC ATGATGCGGT CCAGTCCCTT TATAGTTCTG
    TATGCTGAGG CCCTTTTGAT TGCCCAATAC ATATACGGCA TGGATCTCAA CAACGAAGAG
    CTGCCCACGA GCGTTCCCAC TGCGGGCATT AACCTGCAAC AAATTGGCTT CGAACGACCC
    ATCGAGAACC AAATGCGGCC ATGTGTGCCG CTGATCGTGA AGACAGCGTT TGTGCTAATG
    TTTTGGGTGA CATCACGACA GTTCTTCAAG GAGAAGCGCG ATCGCCGACG GGACAGCACT
    CTGGCGGACT TCATTGCCCC ACTGCAGATT ACTGTGGGAT CGGCTGGCTC CAGCTACCTC
    ATCAACGATG GCAAGAAGAC CTCAAAGTTC CTAAAGAAGG CCGGCGATGT GATCAAAAAT
    CTATTGGTGC GTCTGTGGAT CTGGCTACTC GTGCTGGTTA TCTTCCTTTG CGCCATCACT
    GGCGAGAACA TGACCGGCTT CCGCATCTGC TACATGGCCC TGTTTCTATT CTTCTTGCTA
    GTCTTTCAAT CTTCGTCCAA GGCGTGGGTT AAGATCATGT ACGGCTTCTG GCTGTTTCTG
    ATCTTCTATG CCATGTCCAT ACTTATATTG ATCTACACAT ATCAATTCGA CAAGTTCGAC
    ACGTACTGGA GCGACTATCT TAATGTGTCC GCGACTTTGC AAAAGGACAT CGGCCTTAAG
    CGCTATCAGA CCAAGGATCT GTTCCTCCAT TTGGTCTCAC CGACGATAAT TGTGATCCTG
    ACCGTCATAC AAGTGCACTA CTTCCACAAG CGCTTTATTG CATCATTGCA GCAGCAGCCG
    TTGGCTGGCG GATCGGCACA GCAGAAACCC ACGGAGACAA CTGCCTTGGA ACCGGCGCCA
    TCGAAGCGAC GTGGCAGCGC CGGTTCACTG CGTAAATCTC AGGGTCCATC GGCGGAGGCT
    GCTCCAGGAG CCACCACCGA TTTCGAGACA TCTGTGCGAG ACTTGGTGCG CATATCGTTC
    CGCAAGATCA AGAACAAGTC GGAGTACATC TTCAAGAACT TCAAGGATGT CTTCTGGCGC
    TTCCTGGAGC TGCACATCAT GAAGGCTGTG TATATCGCAG CCTTCGTGTG CAGTGTCAGC
    GAAGTCTGCG TACTGCACAT TATCTTTGTG GGTTTCTGTG TGCTGGGCGC CACCTCGCGG
    AAGGCCGTTC AGGTGGTGAT CAGCCGCCTC ATCTCGTTCA TTGTCACCGT CATAGTTCTG
    TCCAAGATGA TCTACCAGAT CGAGTACTTG AGTCACTCGC AGCACAATGT GGTTTGTTCT
    GACAACCGGA CTGCCAACAA TGCGGAGTGG ATTGGTCTCA CCAAGGCTGA CAAGGTAACT
    GGCGGACTGA TGAGCCTGTT GCGCACCTAC ATCATCTACA TGGTTATTGT GACCATGCAC
    GCAGTGATCA GTTTGCGGCA GCTTCAAATG CGCGTCAAGA TCGGAGCACT GAATGCTCCA
    CCCACCAAGC TGCTGTTCCC CAATATTATT CGAGCTGATG CTGAGAAGGA TCTGGTGGGA
    CTGGTCAAGT ATCTCCTCAA CTTTGGCTTC TACAAATTCG GCATTGAGAT ATCGCTAATC
    GCGCTGGTCT CTACCATCAC ATATCGCCAG GATATTGTTG CCGTAGTCTA TGCTCTGTGG
    CTAGTGGTGC TTTTGCTTCT ACGGAGATCG CAGTGCGCCA AAATATGGGG CGTGTTTCAG
    GCATTCTTTG CCATCTCCAT ACTGACACAG TATATAGTGC TGGTAGGATT GCCGCCGAGC
    TCATGCCTGG TGTTTCCCTG GGATGAAGGT CCCTTCGGCG AGGGCATACA ACGCTGGGCG
    ATGCTGCCAG GAGCCCTGCA CTTCAACCAC GTACCCAAGC TGATCTTCGA CTTTATTGTC
    TTGGTCATTC TGAATCGACA GAAGAGTATC TTCTGCATCG AACAGCGTTA TGCCAGTAAC
    GATGACTACC CGGGTGGCAG CAATCGCAGT GTGATCGCGG ATATTGCTCA GCTAGGTCGC
    GTTCCCTTCG ACAATCCCAC CCACGACTTT TGTTCGTACA TACGCAACTA CTCGGACATC
    CTTAAGAACG GAGTGCTGTG CGGCTTCTAC TGGTTTACTC TGGCAGTTGT TTTCTTGGCC
    GGCACCAATA TTGCTGATCT GCTGGCACTG GGTTATCTGA TCGGAGCGTT TATCTTCCTG
    TGGCAGGGAT CGGATTTCTA TCTGCGTCCC ATACACACCA TCATCTTTCG CTGGAAGTGG
    CTGCTGGCAT TCAATGTGGC CAACATACTT ATCAAGACGT CCTTCCAAAT GGCCGGCTGT
    TTGTTCATGA CCCAACTGAC GAAAGACTGC TGCTGGCTGG TGCACATGCT CGGCATCACA
    TGTACGAGCA ATGTACTTAC AGAGCAAATA ATGCTGCCCG AGGAGGCTGA ATTGGCGCTT
    AAGCCAGGCG AATGTCCTAA GATCACCCAC CAGGTGGTCC TTCTGTGGGA CACGATTTGC
    TTTGCCTTCA TCATCTTCCA GCTGCGCATC TTCAAGTCGC ACTACTTCTG TCACATCATA
    ACGGACACCA AAGCAAATAA CATCCTGGCC TCAAGAGGAG CCGACATCAT TGAGAGCCTA
    CGACATAAGC AGATTGCACA TCGACACGAC CATGAAAAGC AGGTGCTGCA TAAGATCAAG
    CGAAAGATGG AGCGCATCCG TGCCACGCAG CAGAAGATGC TACGACCCTT GGACAAACAA
    ACCCACTTCG ACGAACATGG TTATCCACTT CCTGCACCAA CAGTACGCAG AAGGAAGGAA
    ATTAAATTAC ATCCACATGC TACCCGTGCT GGTGACTACT ATATGTTCGA GGAGATGGAC
    GATAAGTTTG AGCTCGACTT GATACACGAC GAGATCGACT TCCTCGAGGA GGAGAACATC
    ACCGAGAGCG AGATGAAGAT GCAGCGACGC AAGACCCTCT ACGATCTATT ACCAGCCAGT
    GGTCTAACTC GCTATATTTA TTTGAACCCA CAGAAGTCGA AGGATGCACC CACAGGCGAG
    TTTCCCTCCA CCAGTAAGGG TATTTCGAAG GAGCGCGATG CGGCAACGGC TTCCAGTTCG
    GCTAGTCCAG CGCCCACTAG GGATGTGGGT GATCTGCCTG TGATTCCACC ACCCTCCACT
    GGCCTGGGAC GTGAGCAAAC CTCCAAGGAG ACCTCCGATA GCAAGTCCAA AATGGAAGTG
    GACAGCGGAG AGGTGACGGC CAAGGATTCG GATGAGGACT TCGATACCAA TCCCATTATC
    AGACTGCTCG AGGGCTTCTT GGTCACGCTG ACCATAAGAC TGAACCGCTT TTCGCGCAAC
    TATCGCTTTG TGAATCGCAT CCTGGCCGGC GAAAAGAAGA CCCTAAAGGA GTCCAGTTCG
    TTGAACCGTC TGGGGCTGTC CAGTGCCGCT GCCATGTTCC ACTTCCTCAA ATCGAATTTA
    GAGAGAAAAC AATCAATCAA TTCATCGCCG CCAGCCAAGG GCGCGGGTGA ATTCAATCTG
    GAGGAGGAGA ACTTCGCCCA GAGAGATCAT CACATCATTG TCGAGGTGTT GATCTCCTCG
    TGGTATGCCC TGTTGGCCAA CACGGATCTC ATCTGCTACA TTGTGGTTTT CATCAATCAG
    GTGGTCAATG CCAGTCTCAT TTCGCTGCCG CTGCCCATTA TGGTCTTTTT GTGGGGCACA
    TTGTCTCTGC CGCGTCCCAC TAAAACCTTC TGGGTCACGC TGATTGCCTA CACCCAGGCC
    ATCGTGCTGA TCAAGTGCAT CTTCCAGTTT AAGCTGATCT GGTCCAATTA CCACCAACTG
    CCCAATCAGC CGCTAACACC GGCCAAAATA TTCGGCGTTG AGAATAAGGC CCATTATGCG
    ATTTACGACC TGATACTATT GCTGGTTCTA TTCCTGCATC GCTATTTGCT TAAATCACAA
    GGCCTGTGGA AATCGGGCTA CAAGGACACG GATAACCAGT TCACCAAGCC CACCGCTAGC
    ATTGATGAAC GCGACGATAG CGACAACTTA TCCCAACCGG ATTCCCGCCA GCTAAACGAT
    GATGCTGCTC AGAAGCTGAG TCTCCAAGTG AGCCAGGCTT CCTTGCCAGG ATCGCCCGAG
    TTTAGCAAGA CAGGCATCAA TCAGCTAGAG CGCACCAAGT ACACTTCATC GCTGTACAAA
    TTCTTCTTCA GTTTGGTCCA CAAATCCCGT CTAGCCACAG ATGTTTACGC TCTGATGTTC
    CTCTGCGATT TTGTGAACTT CTTCGTGCTT CTTTTCGGCT TCACTGCTTT TGGAACTCAG
    CAAACGGAAA GTGATGAAGG TGTGCAGACA TATCTTGCGG AGAACAAAGT GCCCATACCA
    TTCCTGATTA TGCTACTTGT TCAGTTCCTG CTCATTGTCA TTGATCGAGC TTTGTATCTG
    CGCAAAGCCC TGGTGAACAA GATCATCTTC CACTTCTTTT CGGTTATCGG AATACACATC
    TGGATGTTCT TCGTTGTGCC TGCAGTTACG GAGCGCACTT TCAACTCCCT CGCACCTCCA
    ATAATATTCT ACGTTATCAA GTGCTTTTAC ATGCTGCTCA GCTCTTATCA AATCAAATCC
    GGTTACCCTA AGCGCATTCT AGGCAACTTC TTCACCAAGG GTTTCTCGAT GGTCAATATG
    ATTGCCTTTA AGGTGTACAT GCAGATTCCA TTCCTGTACG AGCTGCGAAC CATATTAGAT
    TGGGTGTGCA TTGACAGCAC CATGACCATT TTCGATTGGC TAAAGATGGA GGACATTTTC
    TCGAATATAT ATCTTATCCG CTGCACCCGG CAGTCAGAGA CTGATTTTCC GGCCATGAGA
    GCTCAGAAGA AGGCCTCACT TTCAAAGCTG ATAATGGGTG GCACCATCGT CCTGCTGATA
    GTGATTTGCA TTTGGGGACC ATTGTGTTTG TTTGCACTTG GCAACGCGGT GGGCACCTCG
    AATGTGCCGT TCCATGTGTC GCTATCAATA CGAATTGGAC CCTATGATCC CATCTACACA
    ACGAACAACT ACGACAGTAT CTTCGAGATC AATCCTGAGA TGTACTCTCA GATGACTAAT
    GCATACATAA AGGAGAAACA GGCTTTGACG TTTATTGCCG GCTATGATGC AACGGATGTA
    GCGGCGGTTA GACTAGCTGG CAACTCGCCA TCTCTGTGGA ACATAGCACC GCCGGATAGG
    CAGCGACTAC TAAATGATTT AAGAAACAAT CACACGCTGA AGGCCCGGTT CTCTTATTCC
    CTCACCCGAA AGGCTCCTGC CAAGGGGCTA AAGGAAAATG TGGGCGACGA GCATGCCATA
    TCGCTGGACG AGTCCTTTGA GGGACGAGCA GCACTCATTC ACATGCTAAG CGAAACCCAT
    GATGTGGAGC CCATTTACAG CAATGGCACC ACAAATGGAA CCACTCCCGA AGTTGAAGAA
    GTGGTGGTGA TACCCGGAAT GATACCCAAG TTTATCAAGG TTCTCAATTC GGGCGACGCT
    GCGGTGGTAA GTGTTTTGAG CCCAAAACAC TACGACTACC GACCGTTGGT TATCAAAATG
    CATCGGGACA ACGAGACCAA TGGGTTGTGG TGGGAGATTC GGGACTATTG CAACGACACC
    TTCTACAACG AAACTCTATC GAAGTTTGCA TACAGCAACT GCACTTCGGG AATTGTGATG
    TACACGTTCA ACGATAAAAA GTTTCCATCC ACGTTCAGTT TCCTCACAGC GGGAGGCATC
    ATTGGTCTGT ACACTACCTT TGTGTTATTG GCCTCGCGCT TTATGAAGTC CTTCATTGGC
    GGGCAAAACA GAAAGATCAT GTTTGAGGAT CTGCCCTATG TTGATAGAGT GCTGCAGCTC
    TGTCTGGACA TTTATCTGGT ACGGGAGGCA TTGGAATTCG CTTTGGAAGA GGACCTGTTT
    GCCAAATTAC TCTTCCTATA CCGATCGCCC GAAACGCTAA TCAAGTGGAC GCGTCCCAAG
    GAGGAGTACG TGGACGATGA TGGCGACACC GACTCGATTC CCAGTCGAAT GAGCGTGCGC
    CGGCCGGAGC AACTGCAGCC ACAGCAACCG CAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001260226.1
    CDS577..8292
    Translation

    Target ORF information:

    RefSeq Version NM_001273297.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster piezo, transcript variant K (Piezo), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001273297.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGTCTTCA GCTATGCGTG CATGGTGCTC CAGCGCATCG TGGTGCCAGC GGTTCTGGTA 
    CTCGCTGCCC TGATGCGACC AGTGGGCATA TCCTTTGTCT ACCTTTTGAT GTTCTTTGTG
    TCGCCCTTTG TTCCGCTGGC CACGCGACGC AACTTCAAAG GATCTGTGAC TGCCTTCTTC
    ATCATCCTGC TGACGTTGAG CACGCTGGTC CTCTTGGGTC ACATAACGCT ACAGATTCTG
    GCGGTCAGCC TCACGCTGCC CATCTACAAT TGCTCGTTCA GTGAGCGTCT GCTGCGTCAC
    ATTGGCTTCG TGAGCTTTAT TGATCTACAG CCATTTGCCA TCATCGAATG GCTAGTGCCT
    GAGGTGTTGG TTTTCGCCAC CTCCCTGGGT TCGTATCTCA CGGTGAAGCG AGTGGCCTCA
    CAGCCCGTCG GCGCCGAGCA GCTGGAGAAC GGCGAGGTGG TCGATGGCCA GGCGGAAAAC
    GCGCAGACAT CTTCTCAGCC ATCTGCCGCA GATGCCAATG GAGGAGATGT GCAACAGGCC
    ACGGTCACCA CGCCGCTGCA GCAACAGCAG CAGCAGCTGA GGAAACGAGT GTCCATGATC
    AGCCAGCACA TACACTTTGA GGGCTTGGTC AAGATCTCTC CTCTGTTCTG CCTGGCCACG
    CTGTTCTTTG CGGCCGTACT GCGTCCCTCG GTGCCTGGCG GATTTTACTT TCTCATTTTC
    CTGCTGTCCG GCACCTACTG GGCAACATGC CAGACGCTGC AACGGGGCTT CGCTTTGTTG
    CTGCGCTGCG TGATGGTCGT CCTCGTGCTG CACTCCCTGT CCATTGTGTC ATACCAGACG
    CCATGGATGC AGAGCCACCT CAATCATACC ACCCTGACAG CTCGATTGAT TGGACTGGAA
    CCGCTTATTG AATCCTACTG CTCGCCGGAT ATACGAGTCT TTCTGTACAA TAATAAGCTA
    TCCCTGGACT CGTACCTCAA TCCCTTTGCG TTGTTCTTTG CCTATTTCGC ACTGGCACTG
    ACCACCAAGC ATCTGATTAA GCCACGGCTG GAGCCCAAGC CCGCCACCGC ACTTGGGCAG
    CAACTAGATT GCAATAGCAG CAGCATCAAC AACACCACCG GCAACAAGGT CAACCGCCAG
    CTATCGCTGC TCACCTCGCA GACATCGCGG GGTCGTCGGG ATGGGTCGAA TCCTGGCGGA
    GGTGGAACGA CCATTACCAC CACCACGACC ACCACCTCCA CCACTGCAAT CCGCAATCAG
    CGCTTGAGTG TTTCTTTGCG CCGAGATCAG CGTGCAACGT TAAATGAACC GACTGAGACG
    ACGCCTTTGG TGCGGCAAAG CACGCGAAAG GCACGTACAC CCCAACCGCT GGAAAGTGGA
    TCCTCGGTGG CGCCAAGTGT CACTCAGCGG GGCAACGACA TGCAGCTGGA ATCGATGGAG
    CAGCGATCGG AACAGGAGAA CACCACCACA TCCATACTGG ATCAAATATC GTATGGATTC
    GTCAGCGTGG GAGGATTCAT ATATCAGAAT AGCTATATAT TCACCAATAT TCTTATGATG
    GCCTGGTCCA TAGTGTATCA CAGTTGGCTG ACTTTTGTCC TCTTGCTGTC GGCCAACGTA
    CTGTGGATGA TTCCCAACCA GAGGAAGGCC ATGATGCGGT CCAGTCCCTT TATAGTTCTG
    TATGCTGAGG CCCTTTTGAT TGCCCAATAC ATATACGGCA TGGATCTCAA CAACGAAGAG
    CTGCCCACGA GCGTTCCCAC TGCGGGCATT AACCTGCAAC AAATTGGCTT CGAACGACCC
    ATCGAGAACC AAATGCGGCC ATGTGTGCCG CTGATCGTGA AGACAGCGTT TGTGCTAATG
    TTTTGGGTGA CATCACGACA GTTCTTCAAG GAGAAGCGCG ATCGCCGACG GGACAGCACT
    CTGGCGGACT TCATTGCCCC ACTGCAGATT ACTGTGGGAT CGGCTGGCTC CAGCTACCTC
    ATCAACGATG GCAAGAAGAC CTCAAAGTTC CTAAAGAAGG CCGGCGATGT GATCAAAAAT
    CTATTGGTGC GTCTGTGGAT CTGGCTACTC GTGCTGGTTA TCTTCCTTTG CGCCATCACT
    GGCGAGAACA TGACCGGCTT CCGCATCTGC TACATGGCCC TGTTTCTATT CTTCTTGCTA
    GTCTTTCAAT CTTCGTCCAA GGCGTGGGTT AAGATCATGT ACGGCTTCTG GCTGTTTCTG
    ATCTTCTATG CCATGTCCAT ACTTATATTG ATCTACACAT ATCAATTCGA CAAGTTCGAC
    ACGTACTGGA GCGACTATCT TAATGTGTCC GCGACTTTGC AAAAGGACAT CGGCCTTAAG
    CGCTATCAGA CCAAGGATCT GTTCCTCCAT TTGGTCTCAC CGACGATAAT TGTGATCCTG
    ACCGTCATAC AAGTGCACTA CTTCCACAAG CGCTTTATTG CATCATTGCA GCAGCAGCCG
    TTGGCTGGCG GATCGGCACA GCAGAAACCC ACGGAGACAA CTGCCTTGGA ACCGGCGCCA
    TCGAAGCGAC GTGGCAGCGC CGGTTCACTG CGTAAATCTC AGGGTCCATC GGCGGAGGCT
    GCTCCAGGAG CCACCACCGA TTTCGAGACA TCTGTGCGAG ACTTGGTGCG CATATCGTTC
    CGCAAGATCA AGAACAAGTC GGAGTACATC TTCAAGAACT TCAAGGATGT CTTCTGGCGC
    TTCCTGGAGC TGCACATCAT GAAGGCTGTG TATATCGCAG CCTTCGTGTG CAGTGTCAGC
    GAAGTCTGCG TACTGCACAT TATCTTTGTG GGTTTCTGTG TGCTGGGCGC CACCTCGCGG
    AAGGCCGTTC AGGTGGTGAT CAGCCGCCTC ATCTCGTTCA TTGTCACCGT CATAGTTCTG
    TCCAAGATGA TCTACCAGAT CGAGTACTTG AGTCACTCGC AGCACAATGT GGTTTGTTCT
    GACAACCGGA CTGCCAACAA TGCGGAGTGG ATTGGTCTCA CCAAGGCTGA CAAGGTAACT
    GGCGGACTGA TGAGCCTGTT GCGCACCTAC ATCATCTACA TGGTTATTGT GACCATGCAC
    GCAGTGATCA GTTTGCGGCA GCTTCAAATG CGCGTCAAGA TCGGAGCACT GAATGCTCCA
    CCCACCAAGC TGCTGTTCCC CAATATTATT CGAGCTGATG CTGAGAAGGA TCTGGTGGGA
    CTGGTCAAGT ATCTCCTCAA CTTTGGCTTC TACAAATTCG GCATTGAGAT ATCGCTAATC
    GCGCTGGTCT CTACCATCAC ATATCGCCAG GATATTGTTG CCGTAGTCTA TGCTCTGTGG
    CTAGTGGTGC TTTTGCTTCT ACGGAGATCG CAGTGCGCCA AAATATGGGG CGTGTTTCAG
    GCATTCTTTG CCATCTCCAT ACTGACACAG TATATAGTGC TGGTAGGATT GCCGCCGAGC
    TCATGCCTGG TGTTTCCCTG GGATGAAGGT CCCTTCGGCG AGGGCATACA ACGCTGGGCG
    ATGCTGCCAG GAGCCCTGCA CTTCAACCAC GTACCCAAGC TGATCTTCGA CTTTATTGTC
    TTGGTCATTC TGAATCGACA GAAGAGTATC TTCTGCATCG AACAGCGTTA TGCCAGTAAC
    GATGACTACC CGGGTGGCAG CAATCGCAGT GTGATCGCGG ATATTGCTCA GCTAGGTCGC
    GTTCCCTTCG ACAATCCCAC CCACGACTTT TGTTCGTACA TACGCAACTA CTCGGACATC
    CTTAAGAACG GAGTGCTGTG CGGCTTCTAC TGGTTTACTC TGGCAGTTGT TTTCTTGGCC
    GGCACCAATA TTGCTGATCT GCTGGCACTG GGTTATCTGA TCGGAGCGTT TATCTTCCTG
    TGGCAGGGAT CGGATTTCTA TCTGCGTCCC ATACACACCA TCATCTTTCG CTGGAAGTGG
    CTGCTGGCAT TCAATGTGGC CAACATACTT ATCAAGACGT CCTTCCAAAT GGCCGGCTGT
    TTGTTCATGA CCCAACTGAC GAAAGACTGC TGCTGGCTGG TGCACATGCT CGGCATCACA
    TGTACGAGCA ATGTACTTAC AGAGCAAATA ATGCTGCCCG AGGAGGCTGA ATTGGCGCTT
    AAGCCAGGCG AATGTCCTAA GATCACCCAC CAGGTGGTCC TTCTGTGGGA CACGATTTGC
    TTTGCCTTCA TCATCTTCCA GCTGCGCATC TTCAAGTCGC ACTACTTCTG TCACATCATA
    ACGGACACCA AAGCAAATAA CATCCTGGCC TCAAGAGGAG CCGACATCAT TGAGAGCCTA
    CGACATAAGC AGATTGCACA TCGACACGAC CATGAAAAGC AGGTGCTGCA TAAGATCAAG
    CGAAAGATGG AGCGCATCCG TGCCACGCAG CAGAAGATGC TACGACCCTT GGACAAACAA
    ACCCACTTCG ACGAACATGG TTATCCACTT CCTGCACCAA CAGTACGCAG AAGGAAGGAA
    ATTAAATTAC ATCCACATGC TACCCGTGCT GGTGACTACT ATATGTTCGA GGAGATGGAC
    GATAAGTTTG AGCTCGACTT GATACACGAC GAGATCGACT TCCTCGAGGA GGAGAACATC
    ACCGAGAGCG AGATGAAGAT GCAGCGACGC AAGACCCTCT ACGATCTATT ACCAGCCAGT
    GGTCTAACTC GCTATATTTA TTTGAACCCA CAGAAGTCGA AGGATGCACC CACAGGCGAG
    TTTCCCTCCA CCAGTAAGGG TATTTCGAAG GAGCGCGATG CGGCAACGGC TTCCAGTTCG
    GCTAGTCCAG CGCCCACTAG GGATGTGGGT GATCTGCCTG TGATTCCACC ACCCTCCACT
    GGCCTGGGAC GTGAGCAAAC CTCCAAGGAG ACCTCCGATA GCAAGTCCAA AATGGAAGTG
    GACAGCGGAG AGGTGACGGC CAAGGATTCG GATGAGGACT TCGATACCAA TCCCATTATC
    AGACTGCTCG AGGGCTTCTT GGTCACGCTG ACCATAAGAC TGAACCGCTT TTCGCGCAAC
    TATCGCTTTG TGAATCGCAT CCTGGCCGGC GAAAAGAAGA CCCTAAAGGA GTCCAGTTCG
    TTGAACCGTC TGGGGCTGTC CAGTGCCGCT GCCATGTTCC ACTTCCTCAA ATCGAATTTA
    GAGAGAAAAC AATCAATCAA TTCATCGCCG CCAGCCAAGG GCGCGGGTGA ATTCAATCTG
    GAGGAGGAGA ACTTCGCCCA GAGAGATCAT CACATCATTG TCGAGGTGTT GATCTCCTCG
    TGGTATGCCC TGTTGGCCAA CACGGATCTC ATCTGCTACA TTGTGGTTTT CATCAATCAG
    GTGGTCAATG CCAGTCTCAT TTCGCTGCCG CTGCCCATTA TGGTCTTTTT GTGGGGCACA
    TTGTCTCTGC CGCGTCCCAC TAAAACCTTC TGGGTCACGC TGATTGCCTA CACCCAGGCC
    ATCGTGCTGA TCAAGTGCAT CTTCCAGTTT AAGCTGATCT GGTCCAATTA CCACCAACTG
    CCCAATCAGC CGCTAACACC GGCCAAAATA TTCGGCGTTG AGAATAAGGC CCATTATGCG
    ATTTACGACC TGATACTATT GCTGGTTCTA TTCCTGCATC GCTATTTGCT TAAATCACAA
    GGCCTGTGGA AATCGGGCTA CAAGGACACG GATAACCAGT TCACCAAGCC CACCGCTAGC
    ATTGATGAAC GCGACGATAG CGACAACTTA TCCCAACCGG ATTCCCGCCA GCTAAACGAT
    GATGCTGCTC AGAAGCTGAG TCTCCAAGTG AGCCAGGCTT CCTTGCCAGG ATCGCCCGAG
    TTTAGCAAGA CAGGCATCAA TCAGCTAGAG CGCACCAAGT ACACTTCATC GCTGTACAAA
    TTCTTCTTCA GTTTGGTCCA CAAATCCCGT CTAGCCACAG ATGTTTACGC TCTGATGTTC
    CTCTGCGATT TTGTGAACTT CTTCGTGCTT CTTTTCGGCT TCACTGCTTT TGGAACTCAG
    CAAACGGAAA GTGATGAAGG TGTGCAGACA TATCTTGCGG AGAACAAAGT GCCCATACCA
    TTCCTGATTA TGCTACTTGT TCAGTTCCTG CTCATTGTCA TTGATCGAGC TTTGTATCTG
    CGCAAAGCCC TGGTGAACAA GATCATCTTC CACTTCTTTT CGGTTATCGG AATACACATC
    TGGATGTTCT TCGTTGTGCC TGCAGTTACG GAGCGCACTT TCAACTCCCT CGCACCTCCA
    ATAATATTCT ACGTTATCAA GTGCTTTTAC ATGCTGCTCA GCTCTTATCA AATCAAATCC
    GGTTACCCTA AGCGCATTCT AGGCAACTTC TTCACCAAGG GTTTCTCGAT GGTCAATATG
    ATTGCCTTTA AGGTGTACAT GCAGATTCCA TTCCTGTACG AGCTGCGAAC CATATTAGAT
    TGGGTGTGCA TTGACAGCAC CATGACCATT TTCGATTGGC TAAAGATGGA GGACATTTTC
    TCGAATATAT ATCTTATCCG CTGCACCCGG CAGTCAGAGA CTGATTTTCC GGCCATGAGA
    GCTCAGAAGA AGGCCTCACT TTCAAAGCTG ATAATGGGTG GCACCATCGT CCTGCTGATA
    GTGATTTGCA TTTGGGGACC ATTGTGTTTG TTTGCACTTG GCAACGCGGT GGGCACCTCG
    AATGTGCCGT TCCATGTGTC GCTATCAATA CGAATTGGAC CCTATGATCC CATCTACACA
    ACGAACAACT ACGACAGTAT CTTCGAGATC AATCCTGAGA TGTACTCTCA GATGACTAAT
    GCATACATAA AGGAGAAACA GGCTTTGACG TTTATTGCCG GCTATGATGC AACGGATGTA
    GCGGCGGTTA GACTAGCTGG CAACTCGCCA TCTCTGTGGA ACATAGCACC GCCGGATAGG
    CAGCGACTAC TAAATGATTT AAGAAACAAT CACACGCTGA AGGCCCGGTT CTCTTATTCC
    CTCACCCGAA AGGCTCCTGC CAAGGGGCTA AAGGAAAATG TGGGCGACGA GCATGCCATA
    TCGCTGGACG AGTCCTTTGA GGGACGAGCA GCACTCATTC ACATGCTAAG CGAAACCCAT
    GATGTGGAGC CCATTTACAG CAATGGCACC ACAAATGGAA CCACTCCCGA AGTTGAAGAA
    GTGGTGGTGA TACCCGGAAT GATACCCAAG TTTATCAAGG TTCTCAATTC GGGCGACGCT
    GCGGTGGTAA GTGTTTTGAG CCCAAAACAC TACGACTACC GACCGTTGGT TATCAAAATG
    CATCGGGACA ACGAGACCAA TGGGTTGTGG TGGGAGATTC GGGACTATTG CAACGACACC
    TTCTACAACG AAACTCTATC GAAGTTTGCA TACAGCAACT GCACTTCGGG AATTGTGATG
    TACACGTTCA ACGATAAAAA GTTTCCATCC ACGTTCAGTT TCCTCACAGC GGGAGGCATC
    ATTGGTCTGT ACACTACCTT TGTGTTATTG GCCTCGCGCT TTATGAAGTC CTTCATTGGC
    GGGCAAAACA GAAAGATCAT GTTTGAGGAT CTGCCCTATG TTGATAGAGT GCTGCAGCTC
    TGTCTGGACA TTTATCTGGT ACGGGAGGCA TTGGAATTCG CTTTGGAAGA GGACCTGTTT
    GCCAAATTAC TCTTCCTATA CCGATCGCCC GAAACGCTAA TCAAGTGGAC GCGTCCCAAG
    GAGGAGTACG TGGACGATGA TGGCGACACC GACTCGATTC CCAGTCGAAT GAGCGTGCGC
    CGGCCGGAGC AACTGCAGCC ACAGCAACCG CAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa15941
    Clone ID Related Accession (Same CDS sequence) NM_001273299.1
    Accession Version NM_001273299.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7602bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product piezo, isoform M
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    ATGGTCTTCA GCTATGCGTG CATGGTGCTC CAGCGCATCG TGGTGCCAGC GGTTCTGGTA 
    CTCGCTGCCC TGATGCGACC AGTGGGCATA TCCTTTGTCT ACCTTTTGAT GTTCTTTGTG
    TCGCCCTTTG TTCCGCTGGC CACGCGACGC AACTTCAAAG GATCTGTGAC TGCCTTCTTC
    ATCATCCTGC TGACGTTGAG CACGCTGGTC CTCTTGGGTC ACATAACGCT ACAGATTCTG
    GCGGTCAGCC TCACGCTGCC CATCTACAAT TGCTCGTTCA GTGAGCGTCT GCTGCGTCAC
    ATTGGCTTCG TGAGCTTTAT TGATCTACAG CCATTTGCCA TCATCGAATG GCTAGTGCCT
    GAGGTGTTGG TTTTCGCCAC CTCCCTGGGT TCGTATCTCA CGGTGAAGCG AGTGGCCTCA
    CAGCCCGTCG GCGCCGAGCA GCTGGAGAAC GGCGAGGTGG TCGATGGCCA GGCGGAAAAC
    GCGCAGACAT CTTCTCAGCC ATCTGCCGCA GATGCCAATG GAGGAGATGT GCAACAGGCC
    ACGGTCACCA CGCCGCTGCA GCAACAGCAG CAGCAGCTGA GGAAACGAGT GTCCATGATC
    AGCCAGCACA TACACTTTGA GGGCTTGGTC AAGATCTCTC CTCTGTTCTG CCTGGCCACG
    CTGTTCTTTG CGGCCGTACT GCGTCCCTCG GTGCCTGGCG GATTTTACTT TCTCATTTTC
    CTGCTGTCCG GCACCTACTG GGCAACATGC CAGACGCTGC AACGGGGCTT CGCTTTGTTG
    CTGCGCTGCG TGATGGTCGT CCTCGTGCTG CACTCCCTGT CCATTGTGTC ATACCAGACG
    CCATGGATGC AGAGCCACCT CAATCATACC ACCCTGACAG CTCGATTGAT TGGACTGGAA
    CCGCTTATTG AATCCTACTG CTCGCCGGAT ATACGAGTCT TTCTGTACAA TAATAAGCTA
    TCCCTGGACT CGTACCTCAA TCCCTTTGCG TTGTTCTTTG CCTATTTCGC ACTGGCACTG
    ACCACCAAGC ATCTGATTAA GCCACGGTTG GTGCGGCAAA GCACGCGAAA GGCACGTACA
    CCCCAACCGC TGGAAAGTGG ATCCTCGGTG GCGCCAAGTG TCACTCAGCG GGGCAACGAC
    ATGCAGCTGG AATCGATGGA GCAGCGATCG GAACAGGAGA ACACCACCAC ATCCATACTG
    GATCAAATAT CGTATGGATT CGTCAGCGTG GGAGGATTCA TATATCAGAA TAGCTATATA
    TTCACCAATA TTCTTATGAT GGCCTGGTCC ATAGTGTATC ACAGTTGGCT GACTTTTGTC
    CTCTTGCTGT CGGCCAACGT ACTGTGGATG ATTCCCAACC AGAGGAAGGC CATGATGCGG
    TCCAGTCCCT TTATAGTTCT GTATGCTGAG GCCCTTTTGA TTGCCCAATA CATATACGGC
    ATGGATCTCA ACAACGAAGA GCTGCCCACG AGCGTTCCCA CTGCGGGCAT TAACCTGCAA
    CAAATTGGCT TCGAACGACC CATCGAGAAC CAAATGCGGC CATGTGTGCC GCTGATCGTG
    AAGACAGCGT TTGTGCTAAT GTTTTGGGTG ACATCACGAC AGTTCTTCAA GGAGAAGCGC
    GATCGCCGAC GGGACAGCAC TCTGGCGGAC TTCATTGCCC CACTGCAGAT TACTGTGGGA
    TCGGCTGGCT CCAGCTACCT CATCAACGAT GGCAAGAAGA CCTCAAAGTT CCTAAAGAAG
    GCCGGCGATG TGATCAAAAA TCTATTGGTG CGTCTGTGGA TCTGGCTACT CGTGCTGGTT
    ATCTTCCTTT GCGCCATCAC TGGCGAGAAC ATGACCGGCT TCCGCATCTG CTACATGGCC
    CTGTTTCTAT TCTTCTTGCT AGTCTTTCAA TCTTCGTCCA AGGCGTGGGT TAAGATCATG
    TACGGCTTCT GGCTGTTTCT GATCTTCTAT GCCATGTCCA TACTTATATT GATCTACACA
    TATCAATTCG ACAAGTTCGA CACGTACTGG AGCGACTATC TTAATGTGTC CGCGACTTTG
    CAAAAGGACA TCGGCCTTAA GCGCTATCAG ACCAAGGATC TGTTCCTCCA TTTGGTCTCA
    CCGACGATAA TTGTGATCCT GACCGTCATA CAAGTGCACT ACTTCCACAA GCGCTTTATT
    GCATCATTGC AGCAGCAGCC GTTGGCTGGC GGATCGGCAC AGCAGAAACC CACGGAGACA
    ACTGCCTTGG AACCGGCGCC ATCGAAGCGA CGTGGCAGCG CCGGTTCACT GCGTAAATCT
    CAGGGTCCAT CGGCGGAGGC TGCTCCAGGA GCCACCACCG ATTTCGAGAC ATCTGTGCGA
    GACTTGGTGC GCATATCGTT CCGCAAGATC AAGAACAAGT CGGAGTACAT CTTCAAGAAC
    TTCAAGGATG TCTTCTGGCG CTTCCTGGAG CTGCACATCA TGAAGGCTGT GTATATCGCA
    GCCTTCGTGT GCAGTGTCAG CGAAGTCTGC GTACTGCACA TTATCTTTGT GGGTTTCTGT
    GTGCTGGGCG CCACCTCGCG GAAGGCCGTT CAGGTGGTGA TCAGCCGCCT CATCTCGTTC
    ATTGTCACCG TCATAGTTCT GTCCAAGATG ATCTACCAGA TCGAGTACTT GAGTCACTCG
    CAGCACAATG TGGTTTGTTC TGACAACCGG ACTGCCAACA ATGCGGAGTG GATTGGTCTC
    ACCAAGGCTG ACAAGGTAAC TGGCGGACTG ATGAGCCTGT TGCGCACCTA CATCATCTAC
    ATGGTTATTG TGACCATGCA CGCAGTGATC AGTTTGCGGC AGCTTCAAAT GCGCGTCAAG
    ATCGGAGCAC TGAATGCTCC ACCCACCAAG CTGCTGTTCC CCAATATTAT TCGAGCTGAT
    GCTGAGAAGG ATCTGGTGGG ACTGGTCAAG TATCTCCTCA ACTTTGGCTT CTACAAATTC
    GGCATTGAGA TATCGCTAAT CGCGCTGGTC TCTACCATCA CATATCGCCA GGATATTGTT
    GCCGTAGTCT ATGCTCTGTG GCTAGTGGTG CTTTTGCTTC TACGGAGATC GCAGTGCGCC
    AAAATATGGG GCGTGTTTCA GGCATTCTTT GCCATCTCCA TACTGACACA GTATATAGTG
    CTGGTAGGAT TGCCGCCGAG CTCATGCCTG GTGTTTCCCT GGGATGAAGG TCCCTTCGGC
    GAGGGCATAC AACGCTGGGC GATGCTGCCA GGAGCCCTGC ACTTCAACCA CGTACCCAAG
    CTGATCTTCG ACTTTATTGT CTTGGTCATT CTGAATCGAC AGAAGAGTAT CTTCTGCATC
    GAACAGCGTT ATGCCAGTAA CGATGACTAC CCGGGTGGCA GCAATCGCAG TGTGATCGCG
    GATATTGCTC AGCTAGGTCG CGTTCCCTTC GACAATCCCA CCCACGACTT TTGTTCGTAC
    ATACGCAACT ACTCGGACAT CCTTAAGAAC GGAGTGCTGT GCGGCTTCTA CTGGTTTACT
    CTGGCAGTTG TTTTCTTGGC CGGCACCAAT ATTGCTGATC TGCTGGCACT GGGTTATCTG
    ATCGGAGCGT TTATCTTCCT GTGGCAGGGA TCGGATTTCT ATCTGCGTCC CATACACACC
    ATCATCTTTC GCTGGAAGTG GCTGCTGGCA TTCAATGTGG CCAACATACT TATCAAGACG
    TCCTTCCAAA TGGCCGGCTG TTTGTTCATG ACCCAACTGA CGAAAGACTG CTGCTGGCTG
    GTGCACATGC TCGGCATCAC ATGTACGAGC AATGTACTTA CAGAGCAAAT AATGCTGCCC
    GAGGAGGCTG AATTGGCGCT TAAGCCAGGC GAATGTCCTA AGATCACCCA CCAGGTGGTC
    CTTCTGTGGG ACACGATTTG CTTTGCCTTC ATCATCTTCC AGCTGCGCAT CTTCAAGTCG
    CACTACTTCT GTCACATCAT AACGGACACC AAAGCAAATA ACATCCTGGC CTCAAGAGGA
    GCCGACATCA TTGAGAGCCT ACGACATAAG CAGATTGCAC ATCGACACGA CCATGAAAAG
    CAGGTGCTGC ATAAGATCAA GCGAAAGATG GAGCGCATCC GTGCCACGCA GCAGAAGATG
    CTACGACCCT TGGACAAACA AACCCACTTC GACGAACATG GTTATCCACT TCCTGCACCA
    ACAGTACGCA GAAGGAAGGA AATTAAATTA CATCCACATG CTACCCGTGC TGGTGACTAC
    TATATGTTCG AGGAGATGGA CGATAAGTTT GAGCTCGACT TGATACACGA CGAGATCGAC
    TTCCTCGAGG AGGAGAACAT CACCGAGAGC GAGATGAAGA TGCAGCGACG CAAGACCCTC
    TACGATGTGT GGAAGGATCT GAATGATGCC GAGTATCGTC GCCACTTCTA TATGCGTGAG
    CGTTCCTACG CCTCAGAGCC CGGCTCACGC ATTCTCCTGA AGTTGGACGA CGAGAAGGAG
    AAGATTGGGC CCGATCTCGG CCTGGCCAGC GACCACGATG AGGACGACGA TACCATTGAG
    TGCGACCTTG GCATGCGCAC CTCGACGCTG TCCGAACCGC TCGACTTTGG TCGTCGCAGC
    CAGACGCTGC TCGAGAAGTC GAAGGATGCA CCCACAGGCG AGTTTCCCTC CACCAGTAAG
    GGTATTTCGA AGGAGCGCGA TGCGGCAACG GCTTCCAGTT CGGCTAGTCC AGCGCCCACT
    AGGGATGTGG GTGATCTGCC TGTGATTCCA CCACCCTCCA CTGGCCTGGG ACGTGAGCAA
    ACCTCCAAGG AGACCTCCGA TAGCAAGTCC AAAATGGAAG TGGACAGCGG AGAGGTGACG
    GCCAAGGATT CGGATGAGGA CTTCGATACC AATCCCATTA TCAGACTGCT CGAGGGCTTC
    TTGGTCACGC TGACCATAAG ACTGAACCGC TTTTCGCGCA ACTATCGCTT TGTGAATCGC
    ATCCTGGCCG GCGAAAAGAA GACCCTAAAG GAGTCCAGTT CGTTGAACCG TCTGGGGCTG
    TCCAGTGCCG CTGCCATGTT CCACTTCCTC AAATCGAATT TAGAGAGCGC GGGTGAATTC
    AATCTGGAGG AGGAGAACTT CGCCCAGAGA GATCATCACA TCATTGTCGA GGTGTTGATC
    TCCTCGTGGT ATGCCCTGTT GGCCAACACG GATCTCATCT GCTACATTGT GGTTTTCATC
    AATCAGGTGG TCAATGCCAG TCTCATTTCG CTGCCGCTGC CCATTATGGT CTTTTTGTGG
    GGCACATTGT CTCTGCCGCG TCCCACTAAA ACCTTCTGGG TCACGCTGAT TGCCTACACC
    CAGGCCATCG TGCTGATCAA GTGCATCTTC CAGTTTAAGC TGATCTGGTC CAATTACCAC
    CAACTGCCCA ATCAGCCGCT AACACCGGCC AAAATATTCG GCGTTGAGAA TAAGGCCCAT
    TATGCGATTT ACGACCTGAT ACTATTGCTG GTTCTATTCC TGCATCGCTA TTTGCTTAAA
    TCACAAGGCC TGTGGAAATC GGGCTACAAG GACACGGATA ACCAGTTCAC CAAGCCCACC
    GCTAGCATTG ATGAACGCGA CGATAGCGAC AACTTATCCC AACCGGATTC CCGCCAGCTA
    AACGATGATG CTGCTCAGAA GCTGAGTCTC CAAGTGAGCC AGGCTTCCTT GCCAGGATCG
    CCCGAGTTTA GCAAGACAGG CATCAATCAG CTAGAGCGCA CCAAGTACAC TTCATCGCTG
    TACAAATTCT TCTTCAGTTT GGTCCACAAA TCCCGTCTAG CCACAGATGT TTACGCTCTG
    ATGTTCCTCT GCGATTTTGT GAACTTCTTC GTGCTTCTTT TCGGCTTCAC TGCTTTTGGA
    ACTCAGCAAA CGGAAAGTGA TGAAGGTGTG CAGACATATC TTGCGGAGAA CAAAGTGCCC
    ATACCATTCC TGATTATGCT ACTTGTTCAG TTCCTGCTCA TTGTCATTGA TCGAGCTTTG
    TATCTGCGCA AAGCCCTGGT GAACAAGATC ATCTTCCACT TCTTTTCGGT TATCGGAATA
    CACATCTGGA TGTTCTTCGT TGTGCCTGCA GTTACGGAGC GCACTTTCAA CTCCCTCGCA
    CCTCCAATAA TATTCTACGT TATCAAGTGC TTTTACATGC TGCTCAGCTC TTATCAAATC
    AAATCCGGTT ACCCTAAGCG CATTCTAGGC AACTTCTTCA CCAAGGGTTT CTCGATGGTC
    AATATGATTG CCTTTAAGGT GTACATGCAG ATTCCATTCC TGTACGAGCT GCGAACCATA
    TTAGATTGGG TGTGCATTGA CAGCACCATG ACCATTTTCG ATTGGCTAAA GATGGAGGAC
    ATTTTCTCGA ATATATATCT TATCCGCTGC ACCCGGCAGT CAGAGACTGA TTTTCCGGCC
    ATGAGAGCTC AGAAGAAGGC CTCACTTTCA AAGCTGATAA TGGGTGGCAC CATCGTCCTG
    CTGATAGTGA TTTGCATTTG GGGACCATTG TGTTTGTTTG CACTTGGCAA CGCGGTGGGC
    ACCTCGAATG TGCCGTTCCA TGTGTCGCTA TCAATACGAA TTGGACCCTA TGATCCCATC
    TACACAACGA ACAACTACGA CAGTATCTTC GAGATCAATC CTGAGATGTA CTCTCAGATG
    ACTAATGCAT ACATAAAGGA GAAACAGGCT TTGACGTTTA TTGCCGGCTA TGATGCAACG
    GATGTAGCGG CGGTTAGACT AGCTGGCAAC TCGCCATCTC TGTGGAACAT AGCACCGCCG
    GATAGGCAGC GACTACTAAA TGATTTAAGA AACAATCACA CGCTGAAGGC CCGGTTCTCT
    TATTCCCTCA CCCGAAAGGC TCCTGCCAAG GGGCTAAAGG AAAATGTGGG CGACGAGCAT
    GCCATATCGC TGGACGAGTC CTTTGAGGGA CGAGCAGCAC TCATTCACAT GCTAAGCGAA
    ACCCATGATG TGGAGCCCAT TTACAGCAAT GGCACCACAA ATGGAACCAC TCCCGAAGTT
    GAAGAAGTGG TGGTGATACC CGGAATGATA CCCAAGTTTA TCAAGGTTCT CAATTCGGGC
    GACGCTGCGG TGGTAAGTGT TTTGAGCCCA AAACACTACG ACTACCGACC GTTGGTTATC
    AAAATGCATC GGGACAACGA GACCAATGGG TTGTGGTGGG AGATTCGGGA CTATTGCAAC
    GACACCTTCT ACAACGAAAC TCTATCGAAG TTTGCATACA GCAACTGCAC TTCGGGAATT
    GTGATGTACA CGTTCAACGA TAAAAAGTTT CCATCCACGT TCAGTTTCCT CACAGCGGGA
    GGCATCATTG GTCTGTACAC TACCTTTGTG TTATTGGCCT CGCGCTTTAT GAAGTCCTTC
    ATTGGCGGGC AAAACAGAAA GATCATGTTT GAGGATCTGC CCTATGTTGA TAGAGTGCTG
    CAGCTCTGTC TGGACATTTA TCTGGTACGG GAGGCATTGG AATTCGCTTT GGAAGAGGAC
    CTGTTTGCCA AATTACTCTT CCTATACCGA TCGCCCGAAA CGCTAATCAA GTGGACGCGT
    CCCAAGGAGG AGTACGTGGA CGATGATGGC GACACCGACT CGATTCCCAG TCGAATGAGC
    GTGCGCCGGC CGGAGCAACT GCAGCCACAG CAACCGCAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001260228.1
    CDS577..8178
    Translation

    Target ORF information:

    RefSeq Version NM_001273299.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster piezo, transcript variant M (Piezo), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001273299.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    ATGGTCTTCA GCTATGCGTG CATGGTGCTC CAGCGCATCG TGGTGCCAGC GGTTCTGGTA 
    CTCGCTGCCC TGATGCGACC AGTGGGCATA TCCTTTGTCT ACCTTTTGAT GTTCTTTGTG
    TCGCCCTTTG TTCCGCTGGC CACGCGACGC AACTTCAAAG GATCTGTGAC TGCCTTCTTC
    ATCATCCTGC TGACGTTGAG CACGCTGGTC CTCTTGGGTC ACATAACGCT ACAGATTCTG
    GCGGTCAGCC TCACGCTGCC CATCTACAAT TGCTCGTTCA GTGAGCGTCT GCTGCGTCAC
    ATTGGCTTCG TGAGCTTTAT TGATCTACAG CCATTTGCCA TCATCGAATG GCTAGTGCCT
    GAGGTGTTGG TTTTCGCCAC CTCCCTGGGT TCGTATCTCA CGGTGAAGCG AGTGGCCTCA
    CAGCCCGTCG GCGCCGAGCA GCTGGAGAAC GGCGAGGTGG TCGATGGCCA GGCGGAAAAC
    GCGCAGACAT CTTCTCAGCC ATCTGCCGCA GATGCCAATG GAGGAGATGT GCAACAGGCC
    ACGGTCACCA CGCCGCTGCA GCAACAGCAG CAGCAGCTGA GGAAACGAGT GTCCATGATC
    AGCCAGCACA TACACTTTGA GGGCTTGGTC AAGATCTCTC CTCTGTTCTG CCTGGCCACG
    CTGTTCTTTG CGGCCGTACT GCGTCCCTCG GTGCCTGGCG GATTTTACTT TCTCATTTTC
    CTGCTGTCCG GCACCTACTG GGCAACATGC CAGACGCTGC AACGGGGCTT CGCTTTGTTG
    CTGCGCTGCG TGATGGTCGT CCTCGTGCTG CACTCCCTGT CCATTGTGTC ATACCAGACG
    CCATGGATGC AGAGCCACCT CAATCATACC ACCCTGACAG CTCGATTGAT TGGACTGGAA
    CCGCTTATTG AATCCTACTG CTCGCCGGAT ATACGAGTCT TTCTGTACAA TAATAAGCTA
    TCCCTGGACT CGTACCTCAA TCCCTTTGCG TTGTTCTTTG CCTATTTCGC ACTGGCACTG
    ACCACCAAGC ATCTGATTAA GCCACGGTTG GTGCGGCAAA GCACGCGAAA GGCACGTACA
    CCCCAACCGC TGGAAAGTGG ATCCTCGGTG GCGCCAAGTG TCACTCAGCG GGGCAACGAC
    ATGCAGCTGG AATCGATGGA GCAGCGATCG GAACAGGAGA ACACCACCAC ATCCATACTG
    GATCAAATAT CGTATGGATT CGTCAGCGTG GGAGGATTCA TATATCAGAA TAGCTATATA
    TTCACCAATA TTCTTATGAT GGCCTGGTCC ATAGTGTATC ACAGTTGGCT GACTTTTGTC
    CTCTTGCTGT CGGCCAACGT ACTGTGGATG ATTCCCAACC AGAGGAAGGC CATGATGCGG
    TCCAGTCCCT TTATAGTTCT GTATGCTGAG GCCCTTTTGA TTGCCCAATA CATATACGGC
    ATGGATCTCA ACAACGAAGA GCTGCCCACG AGCGTTCCCA CTGCGGGCAT TAACCTGCAA
    CAAATTGGCT TCGAACGACC CATCGAGAAC CAAATGCGGC CATGTGTGCC GCTGATCGTG
    AAGACAGCGT TTGTGCTAAT GTTTTGGGTG ACATCACGAC AGTTCTTCAA GGAGAAGCGC
    GATCGCCGAC GGGACAGCAC TCTGGCGGAC TTCATTGCCC CACTGCAGAT TACTGTGGGA
    TCGGCTGGCT CCAGCTACCT CATCAACGAT GGCAAGAAGA CCTCAAAGTT CCTAAAGAAG
    GCCGGCGATG TGATCAAAAA TCTATTGGTG CGTCTGTGGA TCTGGCTACT CGTGCTGGTT
    ATCTTCCTTT GCGCCATCAC TGGCGAGAAC ATGACCGGCT TCCGCATCTG CTACATGGCC
    CTGTTTCTAT TCTTCTTGCT AGTCTTTCAA TCTTCGTCCA AGGCGTGGGT TAAGATCATG
    TACGGCTTCT GGCTGTTTCT GATCTTCTAT GCCATGTCCA TACTTATATT GATCTACACA
    TATCAATTCG ACAAGTTCGA CACGTACTGG AGCGACTATC TTAATGTGTC CGCGACTTTG
    CAAAAGGACA TCGGCCTTAA GCGCTATCAG ACCAAGGATC TGTTCCTCCA TTTGGTCTCA
    CCGACGATAA TTGTGATCCT GACCGTCATA CAAGTGCACT ACTTCCACAA GCGCTTTATT
    GCATCATTGC AGCAGCAGCC GTTGGCTGGC GGATCGGCAC AGCAGAAACC CACGGAGACA
    ACTGCCTTGG AACCGGCGCC ATCGAAGCGA CGTGGCAGCG CCGGTTCACT GCGTAAATCT
    CAGGGTCCAT CGGCGGAGGC TGCTCCAGGA GCCACCACCG ATTTCGAGAC ATCTGTGCGA
    GACTTGGTGC GCATATCGTT CCGCAAGATC AAGAACAAGT CGGAGTACAT CTTCAAGAAC
    TTCAAGGATG TCTTCTGGCG CTTCCTGGAG CTGCACATCA TGAAGGCTGT GTATATCGCA
    GCCTTCGTGT GCAGTGTCAG CGAAGTCTGC GTACTGCACA TTATCTTTGT GGGTTTCTGT
    GTGCTGGGCG CCACCTCGCG GAAGGCCGTT CAGGTGGTGA TCAGCCGCCT CATCTCGTTC
    ATTGTCACCG TCATAGTTCT GTCCAAGATG ATCTACCAGA TCGAGTACTT GAGTCACTCG
    CAGCACAATG TGGTTTGTTC TGACAACCGG ACTGCCAACA ATGCGGAGTG GATTGGTCTC
    ACCAAGGCTG ACAAGGTAAC TGGCGGACTG ATGAGCCTGT TGCGCACCTA CATCATCTAC
    ATGGTTATTG TGACCATGCA CGCAGTGATC AGTTTGCGGC AGCTTCAAAT GCGCGTCAAG
    ATCGGAGCAC TGAATGCTCC ACCCACCAAG CTGCTGTTCC CCAATATTAT TCGAGCTGAT
    GCTGAGAAGG ATCTGGTGGG ACTGGTCAAG TATCTCCTCA ACTTTGGCTT CTACAAATTC
    GGCATTGAGA TATCGCTAAT CGCGCTGGTC TCTACCATCA CATATCGCCA GGATATTGTT
    GCCGTAGTCT ATGCTCTGTG GCTAGTGGTG CTTTTGCTTC TACGGAGATC GCAGTGCGCC
    AAAATATGGG GCGTGTTTCA GGCATTCTTT GCCATCTCCA TACTGACACA GTATATAGTG
    CTGGTAGGAT TGCCGCCGAG CTCATGCCTG GTGTTTCCCT GGGATGAAGG TCCCTTCGGC
    GAGGGCATAC AACGCTGGGC GATGCTGCCA GGAGCCCTGC ACTTCAACCA CGTACCCAAG
    CTGATCTTCG ACTTTATTGT CTTGGTCATT CTGAATCGAC AGAAGAGTAT CTTCTGCATC
    GAACAGCGTT ATGCCAGTAA CGATGACTAC CCGGGTGGCA GCAATCGCAG TGTGATCGCG
    GATATTGCTC AGCTAGGTCG CGTTCCCTTC GACAATCCCA CCCACGACTT TTGTTCGTAC
    ATACGCAACT ACTCGGACAT CCTTAAGAAC GGAGTGCTGT GCGGCTTCTA CTGGTTTACT
    CTGGCAGTTG TTTTCTTGGC CGGCACCAAT ATTGCTGATC TGCTGGCACT GGGTTATCTG
    ATCGGAGCGT TTATCTTCCT GTGGCAGGGA TCGGATTTCT ATCTGCGTCC CATACACACC
    ATCATCTTTC GCTGGAAGTG GCTGCTGGCA TTCAATGTGG CCAACATACT TATCAAGACG
    TCCTTCCAAA TGGCCGGCTG TTTGTTCATG ACCCAACTGA CGAAAGACTG CTGCTGGCTG
    GTGCACATGC TCGGCATCAC ATGTACGAGC AATGTACTTA CAGAGCAAAT AATGCTGCCC
    GAGGAGGCTG AATTGGCGCT TAAGCCAGGC GAATGTCCTA AGATCACCCA CCAGGTGGTC
    CTTCTGTGGG ACACGATTTG CTTTGCCTTC ATCATCTTCC AGCTGCGCAT CTTCAAGTCG
    CACTACTTCT GTCACATCAT AACGGACACC AAAGCAAATA ACATCCTGGC CTCAAGAGGA
    GCCGACATCA TTGAGAGCCT ACGACATAAG CAGATTGCAC ATCGACACGA CCATGAAAAG
    CAGGTGCTGC ATAAGATCAA GCGAAAGATG GAGCGCATCC GTGCCACGCA GCAGAAGATG
    CTACGACCCT TGGACAAACA AACCCACTTC GACGAACATG GTTATCCACT TCCTGCACCA
    ACAGTACGCA GAAGGAAGGA AATTAAATTA CATCCACATG CTACCCGTGC TGGTGACTAC
    TATATGTTCG AGGAGATGGA CGATAAGTTT GAGCTCGACT TGATACACGA CGAGATCGAC
    TTCCTCGAGG AGGAGAACAT CACCGAGAGC GAGATGAAGA TGCAGCGACG CAAGACCCTC
    TACGATGTGT GGAAGGATCT GAATGATGCC GAGTATCGTC GCCACTTCTA TATGCGTGAG
    CGTTCCTACG CCTCAGAGCC CGGCTCACGC ATTCTCCTGA AGTTGGACGA CGAGAAGGAG
    AAGATTGGGC CCGATCTCGG CCTGGCCAGC GACCACGATG AGGACGACGA TACCATTGAG
    TGCGACCTTG GCATGCGCAC CTCGACGCTG TCCGAACCGC TCGACTTTGG TCGTCGCAGC
    CAGACGCTGC TCGAGAAGTC GAAGGATGCA CCCACAGGCG AGTTTCCCTC CACCAGTAAG
    GGTATTTCGA AGGAGCGCGA TGCGGCAACG GCTTCCAGTT CGGCTAGTCC AGCGCCCACT
    AGGGATGTGG GTGATCTGCC TGTGATTCCA CCACCCTCCA CTGGCCTGGG ACGTGAGCAA
    ACCTCCAAGG AGACCTCCGA TAGCAAGTCC AAAATGGAAG TGGACAGCGG AGAGGTGACG
    GCCAAGGATT CGGATGAGGA CTTCGATACC AATCCCATTA TCAGACTGCT CGAGGGCTTC
    TTGGTCACGC TGACCATAAG ACTGAACCGC TTTTCGCGCA ACTATCGCTT TGTGAATCGC
    ATCCTGGCCG GCGAAAAGAA GACCCTAAAG GAGTCCAGTT CGTTGAACCG TCTGGGGCTG
    TCCAGTGCCG CTGCCATGTT CCACTTCCTC AAATCGAATT TAGAGAGCGC GGGTGAATTC
    AATCTGGAGG AGGAGAACTT CGCCCAGAGA GATCATCACA TCATTGTCGA GGTGTTGATC
    TCCTCGTGGT ATGCCCTGTT GGCCAACACG GATCTCATCT GCTACATTGT GGTTTTCATC
    AATCAGGTGG TCAATGCCAG TCTCATTTCG CTGCCGCTGC CCATTATGGT CTTTTTGTGG
    GGCACATTGT CTCTGCCGCG TCCCACTAAA ACCTTCTGGG TCACGCTGAT TGCCTACACC
    CAGGCCATCG TGCTGATCAA GTGCATCTTC CAGTTTAAGC TGATCTGGTC CAATTACCAC
    CAACTGCCCA ATCAGCCGCT AACACCGGCC AAAATATTCG GCGTTGAGAA TAAGGCCCAT
    TATGCGATTT ACGACCTGAT ACTATTGCTG GTTCTATTCC TGCATCGCTA TTTGCTTAAA
    TCACAAGGCC TGTGGAAATC GGGCTACAAG GACACGGATA ACCAGTTCAC CAAGCCCACC
    GCTAGCATTG ATGAACGCGA CGATAGCGAC AACTTATCCC AACCGGATTC CCGCCAGCTA
    AACGATGATG CTGCTCAGAA GCTGAGTCTC CAAGTGAGCC AGGCTTCCTT GCCAGGATCG
    CCCGAGTTTA GCAAGACAGG CATCAATCAG CTAGAGCGCA CCAAGTACAC TTCATCGCTG
    TACAAATTCT TCTTCAGTTT GGTCCACAAA TCCCGTCTAG CCACAGATGT TTACGCTCTG
    ATGTTCCTCT GCGATTTTGT GAACTTCTTC GTGCTTCTTT TCGGCTTCAC TGCTTTTGGA
    ACTCAGCAAA CGGAAAGTGA TGAAGGTGTG CAGACATATC TTGCGGAGAA CAAAGTGCCC
    ATACCATTCC TGATTATGCT ACTTGTTCAG TTCCTGCTCA TTGTCATTGA TCGAGCTTTG
    TATCTGCGCA AAGCCCTGGT GAACAAGATC ATCTTCCACT TCTTTTCGGT TATCGGAATA
    CACATCTGGA TGTTCTTCGT TGTGCCTGCA GTTACGGAGC GCACTTTCAA CTCCCTCGCA
    CCTCCAATAA TATTCTACGT TATCAAGTGC TTTTACATGC TGCTCAGCTC TTATCAAATC
    AAATCCGGTT ACCCTAAGCG CATTCTAGGC AACTTCTTCA CCAAGGGTTT CTCGATGGTC
    AATATGATTG CCTTTAAGGT GTACATGCAG ATTCCATTCC TGTACGAGCT GCGAACCATA
    TTAGATTGGG TGTGCATTGA CAGCACCATG ACCATTTTCG ATTGGCTAAA GATGGAGGAC
    ATTTTCTCGA ATATATATCT TATCCGCTGC ACCCGGCAGT CAGAGACTGA TTTTCCGGCC
    ATGAGAGCTC AGAAGAAGGC CTCACTTTCA AAGCTGATAA TGGGTGGCAC CATCGTCCTG
    CTGATAGTGA TTTGCATTTG GGGACCATTG TGTTTGTTTG CACTTGGCAA CGCGGTGGGC
    ACCTCGAATG TGCCGTTCCA TGTGTCGCTA TCAATACGAA TTGGACCCTA TGATCCCATC
    TACACAACGA ACAACTACGA CAGTATCTTC GAGATCAATC CTGAGATGTA CTCTCAGATG
    ACTAATGCAT ACATAAAGGA GAAACAGGCT TTGACGTTTA TTGCCGGCTA TGATGCAACG
    GATGTAGCGG CGGTTAGACT AGCTGGCAAC TCGCCATCTC TGTGGAACAT AGCACCGCCG
    GATAGGCAGC GACTACTAAA TGATTTAAGA AACAATCACA CGCTGAAGGC CCGGTTCTCT
    TATTCCCTCA CCCGAAAGGC TCCTGCCAAG GGGCTAAAGG AAAATGTGGG CGACGAGCAT
    GCCATATCGC TGGACGAGTC CTTTGAGGGA CGAGCAGCAC TCATTCACAT GCTAAGCGAA
    ACCCATGATG TGGAGCCCAT TTACAGCAAT GGCACCACAA ATGGAACCAC TCCCGAAGTT
    GAAGAAGTGG TGGTGATACC CGGAATGATA CCCAAGTTTA TCAAGGTTCT CAATTCGGGC
    GACGCTGCGG TGGTAAGTGT TTTGAGCCCA AAACACTACG ACTACCGACC GTTGGTTATC
    AAAATGCATC GGGACAACGA GACCAATGGG TTGTGGTGGG AGATTCGGGA CTATTGCAAC
    GACACCTTCT ACAACGAAAC TCTATCGAAG TTTGCATACA GCAACTGCAC TTCGGGAATT
    GTGATGTACA CGTTCAACGA TAAAAAGTTT CCATCCACGT TCAGTTTCCT CACAGCGGGA
    GGCATCATTG GTCTGTACAC TACCTTTGTG TTATTGGCCT CGCGCTTTAT GAAGTCCTTC
    ATTGGCGGGC AAAACAGAAA GATCATGTTT GAGGATCTGC CCTATGTTGA TAGAGTGCTG
    CAGCTCTGTC TGGACATTTA TCTGGTACGG GAGGCATTGG AATTCGCTTT GGAAGAGGAC
    CTGTTTGCCA AATTACTCTT CCTATACCGA TCGCCCGAAA CGCTAATCAA GTGGACGCGT
    CCCAAGGAGG AGTACGTGGA CGATGATGGC GACACCGACT CGATTCCCAG TCGAATGAGC
    GTGCGCCGGC CGGAGCAACT GCAGCCACAG CAACCGCAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa14089
    Clone ID Related Accession (Same CDS sequence) NM_164795.6
    Accession Version NM_164795.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8130bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product piezo, isoform E
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_164795.5. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    ATGGTCTTCA GCTATGCGTG CATGGTGCTC CAGCGCATCG TGGTGCCAGC GGTTCTGGTA 
    CTCGCTGCCC TGATGCGACC AGTGGGCATA TCCTTTGTCT ACCTTTTGAT GTTCTTTGTG
    TCGCCCTTTG TTCCGCTGGC CACGCGACGC AACTTCAAAG GATCTGTGAC TGCCTTCTTC
    ATCATCCTGC TGACGTTGAG CACGCTGGTC CTCTTGGGTC ACATAACGCT ACAGATTCTG
    GCGGTCAGCC TCACGCTGCC CATCTACAAT TGCTCGTTCA GTGAGCGTCT GCTGCGTCAC
    ATTGGCTTCG TGAGCTTTAT TGATCTACAG CCATTTGCCA TCATCGAATG GCTAGTGCCT
    GAGGTGTTGG TTTTCGCCAC CTCCCTGGGT TCGTATCTCA CGGTGAAGCG AGTGGCCTCA
    CAGCCCGTCG GCGCCGAGCA GCTGGAGAAC GGCGAGGTGG TCGATGGCCA GGCGGAAAAC
    GCGCAGACAT CTTCTCAGCC ATCTGCCGCA GATGCCAATG GAGGAGATGT GCAACAGGCC
    ACGGTCACCA CGCCGCTGCA GCAACAGCAG CAGCAGCTGA GGAAACGAGT GTCCATGATC
    AGCCAGCACA TACACTTTGA GGGCTTGGTC AAGATCTCTC CTCTGTTCTG CCTGGCCACG
    CTGTTCTTTG CGGCCGTACT GCGTCCCTCG GTGCCTGGCG GATTTTACTT TCTCATTTTC
    CTGCTGTCCG GCACCTACTG GGCAACATGC CAGACGCTGC AACGGGGCTT CGCTTTGTTG
    CTGCGCTGCG TGATGGTCGT CCTCGTGCTG CACTCCCTGT CCATTGTGTC ATACCAGACG
    CCATGGATGC AGAGCCACCT CAATCATACC ACCCTGACAG CTCGATTGAT TGGACTGGAA
    CCGCTTATTG AATCCTACTG CTCGCCGGAT ATACGAGTCT TTCTGTACAA TAATAAGCTA
    TCCCTGGACT CGTACCTCAA TCCCTTTGCG TTGTTCTTTG CCTATTTCGC ACTGGCACTG
    ACCACCAAGC ATCTGATTAA GCCACGGCTG GAGCCCAAGC CCGCCACCGC ACTTGGGCAG
    CAACTAGATT GCAATAGCAG CAGCATCAAC AACACCACCG GCAACAAGGT CAACCGCCAG
    CTATCGCTGC TCACCTCGCA GACATCGCGG GGTCGTCGGG ATGGGTCGAA TCCTGGCGGA
    GGTGGAACGA CCATTACCAC CACCACGACC ACCACCTCCA CCACTGCAAT CCGCAATCAG
    CGCTTGAGTT TGGTGCGGCA AAGCACGCGA AAGGCACGTA CACCCCAACC GCTGGAAAGT
    GGATCCTCGG TGGCGCCAAG TGTCACTCAG CGGGGCAACG ACATGCAGCT GGAATCGATG
    GAGCAGCGAT CGGAACAGGA GAACACCACC ACATCCATAC TGGATCAAAT ATCGTATGGA
    TTCGTCAGCG TGGGAGGATT CATATATCAG AATAGCTATA TATTCACCAA TATTCTTATG
    ATGGCCTGGT CCATAGTGTA TCACAGTTGG CTGACTTTTG TCCTCTTGCT GTCGGCCAAC
    GTACTGTGGA TGATTCCCAA CCAGAGGAAG GCCATGATGC GGTCCAGTCC CTTTATAGTT
    CTGTATGCTG AGGCCCTTTT GATTGCCCAA TACATATACG GCATGGATCT CAACAACGAA
    GAGCTGCCCA CGAGCGTTCC CACTGCGGGC ATTAACCTGC AACAAATTGG CTTCGAACGA
    CCCATCGAGA ACCAAATGCG GCCATGTGTG CCGCTGATCG TGAAGACAGC GTTTGTGCTA
    ATGTTTTGGG TGACATCACG ACAGTTCTTC AAGGAGAAGC GCGATCGCCG ACGGGACAGC
    ACTCTGGCGG ACTTCATTGC CCCACTGCAG ATTACTGTGG GATCGGCTGG CTCCAGCTAC
    CTCATCAACG ATGGCAAGAA GACCTCAAAG TTCCTAAAGA AGGCCGGCGA TGTGATCAAA
    AATCTATTGG TGCGTCTGTG GATCTGGCTA CTCGTGCTGG TTATCTTCCT TTGCGCCATC
    ACTGGCGAGA ACATGACCGG CTTCCGCATC TGCTACATGG CCCTGTTTCT ATTCTTCTTG
    CTAGTCTTTC AATCTTCGTC CAAGGCGTGG GTTAAGATCA TGTACGGCTT CTGGCTGTTT
    CTGATCTTCT ATGCCATGTC CATACTTATA TTGATCTACA CATATCAATT CGACAAGTTC
    GACACGTACT GGAGCGACTA TCTTAATGTG TCCGCGACTT TGCAAAAGGA CATCGGCCTT
    AAGCGCTATC AGACCAAGGA TCTGTTCCTC CATTTGGTCT CACCGACGAT AATTGTGATC
    CTGACCGTCA TACAAGTGCA CTACTTCCAC AAGCGCTTTA TTGCATCATT GCAGCAGCAG
    CCGTTGGCTG GCGGATCGGC ACAGCAGAAA CCCACGGAGA CAACTGCCTT GGAACCGGCG
    CCATCGAAGC GACGTGGCAG CGCCGGTTCA CTGCGTAAAT CTCAGGGTCC ATCGGCGGAG
    GCTGCTCCAG GAGCCACCAC CGATTTCGAG ACATCTGTGC GAGACTTGGT GCGCATATCG
    TTCCGCAAGA TCAAGAACAA GTCGGAGTAC ATCTTCAAGA ACTTCAAGGA TGTCTTCTGG
    CGCTTCCTGG AGCTGCACAT CATGAAGGCT GTGTATATCG CAGCCTTCGT GTGCAGTGTC
    AGCGAAGTCT GCGTACTGCA CATTATCTTT GTGGGTTTCT GTGTGCTGGG CGCCACCTCG
    CGGAAGGCCG TTCAGGTGGT GATCAGCCGC CTCATCTCGT TCATTGTCAC CGTCATAGTT
    CTGTCCAAGA TGATCTACCA GATCGAGTAC TTGAGTCACT CGCAGCACAA TGTGGTTTGT
    TCTGACAACC GGACTGCCAA CAATGCGGAG TGGATTGGTC TCACCAAGGC TGACAAGGTA
    ACTGGCGGAC TGATGAGCCT GTTGCGCACC TACATCATCT ACATGGTTAT TGTGACCATG
    CACGCAGTGA TCAGTTTGCG GCAGCTTCAA ATGCGCGTCA AGATCGGAGC ACTGAATGCT
    CCACCCACCA AGCTGCTGTT CCCCAATATT ATTCGAGCTG ATGCTGAGAA GGATCTGGTG
    GGACTGGTCA AGTATCTCCT CAACTTTGGC TTCTACAAAT TCGGCATTGA GATATCGCTA
    ATCGCGCTGG TCTCTACCAT CACATATCGC CAGGATATTG TTGCCGTAGT CTATGCTCTG
    TGGCTAGTGG TGCTTTTGCT TCTACGGAGA TCGCAGTGCG CCAAAATATG GGGCGTGTTT
    CAGGCATTCT TTGCCATCTC CATACTGACA CAGTATATAG TGCTGGTAGG ATTGCCGCCG
    AGCTCATGCC TGGTGTTTCC CTGGGATGAA GGTCCCTTCG GCGAGGGCAT ACAACGCTGG
    GCGATGCTGC CAGGAGCCCT GCACTTCAAC CACGTACCCA AGCTGATCTT CGACTTTATT
    GTCTTGGTCA TTCTGAATCG ACAGAAGAGT ATCTTCTGCA TCGAACAGCG TTATGCCAGT
    AACGATGACT ACCCGGGTGG CAGCAATCGC AGTGTGATCG CGGATATTGC TCAGCTAGGT
    CGCGTTCCCT TCGACAATCC CACCCACGAC TTTTGTTCGT ACATACGCAA CTACTCGGAC
    ATCCTTAAGA ACGGAGTGCT GTGCGGCTTC TACTGGTTTA CTCTGGCAGT TGTTTTCTTG
    GCCGGCACCA ATATTGCTGA TCTGCTGGCA CTGGGTTATC TGATCGGAGC GTTTATCTTC
    CTGTGGCAGG GATCGGATTT CTATCTGCGT CCCATACACA CCATCATCTT TCGCTGGAAG
    TGGCTGCTGG CATTCAATGT GGCCAACATA CTTATCAAGA CGTCCTTCCA AATGGCCGGC
    TGTTTGTTCA TGACCCAACT GACGAAAGAC TGCTGCTGGC TGGTGCACAT GCTCGGCATC
    ACATGTACGA GCAATGTACT TACAGAGCAA ATAATGCTGC CCGAGGAGGC TGAATTGGCG
    CTTAAGCCAG GCGAATGTCC TAAGATCACC CACCAGGTGG TCCTTCTGTG GGACACGATT
    TGCTTTGCCT TCATCATCTT CCAGCTGCGC ATCTTCAAGT CGCACTACTT CTGTCACATC
    ATAACGGACA CCAAAGCAAA TAACATCCTG GCCTCAAGAG GAGCCGACAT CATTGAGAGC
    CTACGACATA AGCAGATTGC ACATCGACAC GACCATGAAA AGCAGGTGCT GCATAAGATC
    AAGCGAAAGA TGGAGCGCAT CCGTGCCACG CAGCAGAAGA TGCTACGACC CTTGGACAAA
    CAAACCCACT TCGACGAACA TGGTTATCCA CTTCCTGCAC CAACAGTACG CAGAAGGAAG
    GAAATTAAAT TACATCCACA TGCTACCCGT GCTGGTGACT ACTATATGTT CGAGGAGATG
    GACGATAAGT TTGAGCTCGA CTTGATACAC GACGAGATCG ACTTCCTCGA GGAGGAGAAC
    ATCACCGAGA GCGAGATGAA GATGCAGCGA CGCAAGACCC TCTACGATGT GTGGAAGGAT
    CTGAATGATG CCGAGTATCG TCGCCACTTC TATATGCGTG AGCGTTCCTA CGCCTCAGAG
    CCCGGCTCAC GCATTCTCCT GAAGTTGGAC GACGAGAAGG AGAAGATTGG GCCCGATCTC
    GGCCTGGCCA GCGACCACGA TGAGGACGAC GATACCATTG AGTGCGACCT TGGCATGCGC
    ACCTCGACGC TGTCCGAACC GCTCGACTTT GGTCGTCGCA GCCAGACGCT GCTCGAGGTT
    AAGTCGAAGG ATGCACCCAC AGGCGAGTTT CCCTCCACCA GTAAGGGTAT TTCGAAGGAG
    CGCGATGCGG CAACGGCTTC CAGTTCGGCT AGTCCAGCGC CCACTAGGGA TGTGGGTGAT
    CTGCCTGTGA TTCCACCACC CTCCACTGGC CTGGGACGTG AGCAAACCTC CAAGGAGACC
    TCCGATAGCA AGTCCAAAAT GGAAGTGGAC AGCGGAGAGG TGACGGCCAA GGATTCGGAT
    GAGGACTTCG ATACCAATCC CATTATCAGA CTGCTCGAGG GCTTCTTGGT CACGCTGACC
    ATAAGACTGA ACCGCTTTTC GCGCAACTAT CGCTTTGTGA ATCGCATCCT GGCCGGCGAA
    AAGAAGACCC TAAAGGAGTC CAGTTCGTTG AACCGTCTGG GGCTGTCCAG TGCCGCTGCC
    ATGTTCCACT TCCTCAAATC GAATTTAGAG AGCGATGAAA GTGAACCGCC CGCCTCCTCA
    TCCACCCCGC GGCGGGTGGT GATCGCACCA CCGAATGCCA CCGAGCACTC AGACCCCACC
    AGCACCACAC TGAACACGAA CACGACAACC ACACCGCTAT CACCACCAGA ACCACTGCAA
    CCACTGCAAC CACTGCAACC AAATACAACC AGTACACCAC AGCAACAGCA TCAGCATATT
    CGCGCTGCCG AAGAAATCAT CGAACTGCCT GTAGATACCG TGGATGGAGT CGCCCATAGA
    AAACAATCAA TCAATTCATC GCCGCCAGCC AAGGGCGCGG GTGAATTCAA TCTGGAGGAG
    GAGAACTTCG CCCAGAGAGA TCATCACATC ATTGTCGAGG TGTTGATCTC CTCGTGGTAT
    GCCCTGTTGG CCAACACGGA TCTCATCTGC TACATTGTGG TTTTCATCAA TCAGGTGGTC
    AATGCCAGTC TCATTTCGCT GCCGCTGCCC ATTATGGTCT TTTTGTGGGG CACATTGTCT
    CTGCCGCGTC CCACTAAAAC CTTCTGGGTC ACGCTGATTG CCTACACCCA GGCCATCGTG
    CTGATCAAGT GCATCTTCCA GTTTAAGCTG ATCTGGTCCA ATTACCACCA ACTGCCCAAT
    CAGCCGCTAA CACCGGCCAA AATATTCGGC GTTGAGAATA AGGCCCATTA TGCGATTTAC
    GACCTGATAC TATTGCTGGT TCTATTCCTG CATCGCTATT TGCTTAAATC ACAAGGCCTG
    TGGAAATCGG GCTACAAGGA CACGGATAAC CAGTTCACCA AGCCCACCGC TAGCATTGAT
    GAACGCGACG ATAGCGACAA CTTATCCCAA CCGGATTCCC GCCAGCTAAA CGATGATGCT
    GCTCAGAAGC TGAGTCTCCA AGTGAGCCAG GCTTCCTTGC CAGGATCGCC CGAGTTTAGC
    AAGACAGGCA TCAATCAGCT AGAGCGCACC AAGTACACTT CATCGCTGTA CAAATTCTTC
    TTCAGTTTGG TCCACAAATC CCGTCTAGCC ACAGATGTTT ACGCTCTGAT GTTCCTCTGC
    GATTTTGTGA ACTTCTTCGT GCTTCTTTTC GGCTTCACTG CTTTTGGAAC TCAGCAAACG
    GAAAGTGATG AAGGTGTGCA GACATATCTT GCGGAGAACA AAGTGCCCAT ACCATTCCTG
    ATTATGCTAC TTGTTCAGTT CCTGCTCATT GTCATTGATC GAGCTTTGTA TCTGCGCAAA
    GCCCTGGTGA ACAAGATCAT CTTCCACTTC TTTTCGGTTA TCGGAATACA CATCTGGATG
    TTCTTCGTTG TGCCTGCAGT TACGGAGCGC ACTTTCAACT CCCTCGCACC TCCAATAATA
    TTCTACGTTA TCAAGTGCTT TTACATGCTG CTCAGCTCTT ATCAAATCAA ATCCGGTTAC
    CCTAAGCGCA TTCTAGGCAA CTTCTTCACC AAGGGTTTCT CGATGGTCAA TATGATTGCC
    TTTAAGGTGT ACATGCAGAT TCCATTCCTG TACGAGCTGC GAACCATATT AGATTGGGTG
    TGCATTGACA GCACCATGAC CATTTTCGAT TGGCTAAAGA TGGAGGACAT TTTCTCGAAT
    ATATATCTTA TCCGCTGCAC CCGGCAGTCA GAGACTGATT TTCCGGCCAT GAGAGCTCAG
    AAGAAGGCCT CACTTTCAAA GCTGATAATG GGTGGCACCA TCGTCCTGCT GATAGTGATT
    TGCATTTGGG GACCATTGTG TTTGTTTGCA CTTGGCAACG CGGTGGGCAC CTCGAATGTG
    CCGTTCCATG TGTCGCTATC AATACGAATT GGACCCTATG ATCCCATCTA CACAACGAAC
    AACTACGACA GTATCTTCGA GATCAATCCT GAGATGTACT CTCAGATGAC TAATGCATAC
    ATAAAGGAGA AACAGGCTTT GACGTTTATT GCCGGCTATG ATGCAACGGA TGTAGCGGCG
    GTTAGACTAG CTGGCAACTC GCCATCTCTG TGGAACATAG CACCGCCGGA TAGGCAGCGA
    CTACTAAATG ATTTAAGAAA CAATCACACG CTGAAGGCCC GGTTCTCTTA TTCCCTCACC
    CGAAAGGCTC CTGCCAAGGG GCTAAAGGAA AATGTGGGCG ACGAGCATGC CATATCGCTG
    GACGAGTCCT TTGAGGGACG AGCAGCACTC ATTCACATGC TAAGCGAAAC CCATGATGTG
    GAGCCCATTT ACAGCAATGG CACCACAAAT GGAACCACTC CCGAAGTTGA AGAAGTGGTG
    GTGATACCCG GAATGATACC CAAGTTTATC AAGGTTCTCA ATTCGGGCGA CGCTGCGGTG
    GTAAGTGTTT TGAGCCCAAA ACACTACGAC TACCGACCGT TGGTTATCAA AATGCATCGG
    GACAACGAGA CCAATGGGTT GTGGTGGGAG ATTCGGGACT ATTGCAACGA CACCTTCTAC
    AACGAAACTC TATCGAAGTT TGCATACAGC AACTGCACTT CGGGAATTGT GATGTACACG
    TTCAACGATA AAAAGTTTCC ATCCACGTTC AGTTTCCTCA CAGCGGGAGG CATCATTGGT
    CTGTACACTA CCTTTGTGTT ATTGGCCTCG CGCTTTATGA AGTCCTTCAT TGGCGGGCAA
    AACAGAAAGA TCATGTTTGA GGATCTGCCC TATGTTGATA GAGTGCTGCA GCTCTGTCTG
    GACATTTATC TGGTACGGGA GGCATTGGAA TTCGCTTTGG AAGAGGACCT GTTTGCCAAA
    TTACTCTTCC TATACCGATC GCCCGAAACG CTAATCAAGT GGACGCGTCC CAAGGAGGAG
    TACGTGGACG ATGATGGCGA CACCGACTCG ATTCCCAGTC GAATGAGCGT GCGCCGGCCG
    GAGCAACTGC AGCCACAGCA ACCGCAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_723354.3
    CDS577..8706
    Translation

    Target ORF information:

    RefSeq Version NM_164795.6
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster piezo, transcript variant E (Piezo), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_164795.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    ATGGTCTTCA GCTATGCGTG CATGGTGCTC CAGCGCATCG TGGTGCCAGC GGTTCTGGTA 
    CTCGCTGCCC TGATGCGACC AGTGGGCATA TCCTTTGTCT ACCTTTTGAT GTTCTTTGTG
    TCGCCCTTTG TTCCGCTGGC CACGCGACGC AACTTCAAAG GATCTGTGAC TGCCTTCTTC
    ATCATCCTGC TGACGTTGAG CACGCTGGTC CTCTTGGGTC ACATAACGCT ACAGATTCTG
    GCGGTCAGCC TCACGCTGCC CATCTACAAT TGCTCGTTCA GTGAGCGTCT GCTGCGTCAC
    ATTGGCTTCG TGAGCTTTAT TGATCTACAG CCATTTGCCA TCATCGAATG GCTAGTGCCT
    GAGGTGTTGG TTTTCGCCAC CTCCCTGGGT TCGTATCTCA CGGTGAAGCG AGTGGCCTCA
    CAGCCCGTCG GCGCCGAGCA GCTGGAGAAC GGCGAGGTGG TCGATGGCCA GGCGGAAAAC
    GCGCAGACAT CTTCTCAGCC ATCTGCCGCA GATGCCAATG GAGGAGATGT GCAACAGGCC
    ACGGTCACCA CGCCGCTGCA GCAACAGCAG CAGCAGCTGA GGAAACGAGT GTCCATGATC
    AGCCAGCACA TACACTTTGA GGGCTTGGTC AAGATCTCTC CTCTGTTCTG CCTGGCCACG
    CTGTTCTTTG CGGCCGTACT GCGTCCCTCG GTGCCTGGCG GATTTTACTT TCTCATTTTC
    CTGCTGTCCG GCACCTACTG GGCAACATGC CAGACGCTGC AACGGGGCTT CGCTTTGTTG
    CTGCGCTGCG TGATGGTCGT CCTCGTGCTG CACTCCCTGT CCATTGTGTC ATACCAGACG
    CCATGGATGC AGAGCCACCT CAATCATACC ACCCTGACAG CTCGATTGAT TGGACTGGAA
    CCGCTTATTG AATCCTACTG CTCGCCGGAT ATACGAGTCT TTCTGTACAA TAATAAGCTA
    TCCCTGGACT CGTACCTCAA TCCCTTTGCG TTGTTCTTTG CCTATTTCGC ACTGGCACTG
    ACCACCAAGC ATCTGATTAA GCCACGGCTG GAGCCCAAGC CCGCCACCGC ACTTGGGCAG
    CAACTAGATT GCAATAGCAG CAGCATCAAC AACACCACCG GCAACAAGGT CAACCGCCAG
    CTATCGCTGC TCACCTCGCA GACATCGCGG GGTCGTCGGG ATGGGTCGAA TCCTGGCGGA
    GGTGGAACGA CCATTACCAC CACCACGACC ACCACCTCCA CCACTGCAAT CCGCAATCAG
    CGCTTGAGTT TGGTGCGGCA AAGCACGCGA AAGGCACGTA CACCCCAACC GCTGGAAAGT
    GGATCCTCGG TGGCGCCAAG TGTCACTCAG CGGGGCAACG ACATGCAGCT GGAATCGATG
    GAGCAGCGAT CGGAACAGGA GAACACCACC ACATCCATAC TGGATCAAAT ATCGTATGGA
    TTCGTCAGCG TGGGAGGATT CATATATCAG AATAGCTATA TATTCACCAA TATTCTTATG
    ATGGCCTGGT CCATAGTGTA TCACAGTTGG CTGACTTTTG TCCTCTTGCT GTCGGCCAAC
    GTACTGTGGA TGATTCCCAA CCAGAGGAAG GCCATGATGC GGTCCAGTCC CTTTATAGTT
    CTGTATGCTG AGGCCCTTTT GATTGCCCAA TACATATACG GCATGGATCT CAACAACGAA
    GAGCTGCCCA CGAGCGTTCC CACTGCGGGC ATTAACCTGC AACAAATTGG CTTCGAACGA
    CCCATCGAGA ACCAAATGCG GCCATGTGTG CCGCTGATCG TGAAGACAGC GTTTGTGCTA
    ATGTTTTGGG TGACATCACG ACAGTTCTTC AAGGAGAAGC GCGATCGCCG ACGGGACAGC
    ACTCTGGCGG ACTTCATTGC CCCACTGCAG ATTACTGTGG GATCGGCTGG CTCCAGCTAC
    CTCATCAACG ATGGCAAGAA GACCTCAAAG TTCCTAAAGA AGGCCGGCGA TGTGATCAAA
    AATCTATTGG TGCGTCTGTG GATCTGGCTA CTCGTGCTGG TTATCTTCCT TTGCGCCATC
    ACTGGCGAGA ACATGACCGG CTTCCGCATC TGCTACATGG CCCTGTTTCT ATTCTTCTTG
    CTAGTCTTTC AATCTTCGTC CAAGGCGTGG GTTAAGATCA TGTACGGCTT CTGGCTGTTT
    CTGATCTTCT ATGCCATGTC CATACTTATA TTGATCTACA CATATCAATT CGACAAGTTC
    GACACGTACT GGAGCGACTA TCTTAATGTG TCCGCGACTT TGCAAAAGGA CATCGGCCTT
    AAGCGCTATC AGACCAAGGA TCTGTTCCTC CATTTGGTCT CACCGACGAT AATTGTGATC
    CTGACCGTCA TACAAGTGCA CTACTTCCAC AAGCGCTTTA TTGCATCATT GCAGCAGCAG
    CCGTTGGCTG GCGGATCGGC ACAGCAGAAA CCCACGGAGA CAACTGCCTT GGAACCGGCG
    CCATCGAAGC GACGTGGCAG CGCCGGTTCA CTGCGTAAAT CTCAGGGTCC ATCGGCGGAG
    GCTGCTCCAG GAGCCACCAC CGATTTCGAG ACATCTGTGC GAGACTTGGT GCGCATATCG
    TTCCGCAAGA TCAAGAACAA GTCGGAGTAC ATCTTCAAGA ACTTCAAGGA TGTCTTCTGG
    CGCTTCCTGG AGCTGCACAT CATGAAGGCT GTGTATATCG CAGCCTTCGT GTGCAGTGTC
    AGCGAAGTCT GCGTACTGCA CATTATCTTT GTGGGTTTCT GTGTGCTGGG CGCCACCTCG
    CGGAAGGCCG TTCAGGTGGT GATCAGCCGC CTCATCTCGT TCATTGTCAC CGTCATAGTT
    CTGTCCAAGA TGATCTACCA GATCGAGTAC TTGAGTCACT CGCAGCACAA TGTGGTTTGT
    TCTGACAACC GGACTGCCAA CAATGCGGAG TGGATTGGTC TCACCAAGGC TGACAAGGTA
    ACTGGCGGAC TGATGAGCCT GTTGCGCACC TACATCATCT ACATGGTTAT TGTGACCATG
    CACGCAGTGA TCAGTTTGCG GCAGCTTCAA ATGCGCGTCA AGATCGGAGC ACTGAATGCT
    CCACCCACCA AGCTGCTGTT CCCCAATATT ATTCGAGCTG ATGCTGAGAA GGATCTGGTG
    GGACTGGTCA AGTATCTCCT CAACTTTGGC TTCTACAAAT TCGGCATTGA GATATCGCTA
    ATCGCGCTGG TCTCTACCAT CACATATCGC CAGGATATTG TTGCCGTAGT CTATGCTCTG
    TGGCTAGTGG TGCTTTTGCT TCTACGGAGA TCGCAGTGCG CCAAAATATG GGGCGTGTTT
    CAGGCATTCT TTGCCATCTC CATACTGACA CAGTATATAG TGCTGGTAGG ATTGCCGCCG
    AGCTCATGCC TGGTGTTTCC CTGGGATGAA GGTCCCTTCG GCGAGGGCAT ACAACGCTGG
    GCGATGCTGC CAGGAGCCCT GCACTTCAAC CACGTACCCA AGCTGATCTT CGACTTTATT
    GTCTTGGTCA TTCTGAATCG ACAGAAGAGT ATCTTCTGCA TCGAACAGCG TTATGCCAGT
    AACGATGACT ACCCGGGTGG CAGCAATCGC AGTGTGATCG CGGATATTGC TCAGCTAGGT
    CGCGTTCCCT TCGACAATCC CACCCACGAC TTTTGTTCGT ACATACGCAA CTACTCGGAC
    ATCCTTAAGA ACGGAGTGCT GTGCGGCTTC TACTGGTTTA CTCTGGCAGT TGTTTTCTTG
    GCCGGCACCA ATATTGCTGA TCTGCTGGCA CTGGGTTATC TGATCGGAGC GTTTATCTTC
    CTGTGGCAGG GATCGGATTT CTATCTGCGT CCCATACACA CCATCATCTT TCGCTGGAAG
    TGGCTGCTGG CATTCAATGT GGCCAACATA CTTATCAAGA CGTCCTTCCA AATGGCCGGC
    TGTTTGTTCA TGACCCAACT GACGAAAGAC TGCTGCTGGC TGGTGCACAT GCTCGGCATC
    ACATGTACGA GCAATGTACT TACAGAGCAA ATAATGCTGC CCGAGGAGGC TGAATTGGCG
    CTTAAGCCAG GCGAATGTCC TAAGATCACC CACCAGGTGG TCCTTCTGTG GGACACGATT
    TGCTTTGCCT TCATCATCTT CCAGCTGCGC ATCTTCAAGT CGCACTACTT CTGTCACATC
    ATAACGGACA CCAAAGCAAA TAACATCCTG GCCTCAAGAG GAGCCGACAT CATTGAGAGC
    CTACGACATA AGCAGATTGC ACATCGACAC GACCATGAAA AGCAGGTGCT GCATAAGATC
    AAGCGAAAGA TGGAGCGCAT CCGTGCCACG CAGCAGAAGA TGCTACGACC CTTGGACAAA
    CAAACCCACT TCGACGAACA TGGTTATCCA CTTCCTGCAC CAACAGTACG CAGAAGGAAG
    GAAATTAAAT TACATCCACA TGCTACCCGT GCTGGTGACT ACTATATGTT CGAGGAGATG
    GACGATAAGT TTGAGCTCGA CTTGATACAC GACGAGATCG ACTTCCTCGA GGAGGAGAAC
    ATCACCGAGA GCGAGATGAA GATGCAGCGA CGCAAGACCC TCTACGATGT GTGGAAGGAT
    CTGAATGATG CCGAGTATCG TCGCCACTTC TATATGCGTG AGCGTTCCTA CGCCTCAGAG
    CCCGGCTCAC GCATTCTCCT GAAGTTGGAC GACGAGAAGG AGAAGATTGG GCCCGATCTC
    GGCCTGGCCA GCGACCACGA TGAGGACGAC GATACCATTG AGTGCGACCT TGGCATGCGC
    ACCTCGACGC TGTCCGAACC GCTCGACTTT GGTCGTCGCA GCCAGACGCT GCTCGAGGTT
    AAGTCGAAGG ATGCACCCAC AGGCGAGTTT CCCTCCACCA GTAAGGGTAT TTCGAAGGAG
    CGCGATGCGG CAACGGCTTC CAGTTCGGCT AGTCCAGCGC CCACTAGGGA TGTGGGTGAT
    CTGCCTGTGA TTCCACCACC CTCCACTGGC CTGGGACGTG AGCAAACCTC CAAGGAGACC
    TCCGATAGCA AGTCCAAAAT GGAAGTGGAC AGCGGAGAGG TGACGGCCAA GGATTCGGAT
    GAGGACTTCG ATACCAATCC CATTATCAGA CTGCTCGAGG GCTTCTTGGT CACGCTGACC
    ATAAGACTGA ACCGCTTTTC GCGCAACTAT CGCTTTGTGA ATCGCATCCT GGCCGGCGAA
    AAGAAGACCC TAAAGGAGTC CAGTTCGTTG AACCGTCTGG GGCTGTCCAG TGCCGCTGCC
    ATGTTCCACT TCCTCAAATC GAATTTAGAG AGCGATGAAA GTGAACCGCC CGCCTCCTCA
    TCCACCCCGC GGCGGGTGGT GATCGCACCA CCGAATGCCA CCGAGCACTC AGACCCCACC
    AGCACCACAC TGAACACGAA CACGACAACC ACACCGCTAT CACCACCAGA ACCACTGCAA
    CCACTGCAAC CACTGCAACC AAATACAACC AGTACACCAC AGCAACAGCA TCAGCATATT
    CGCGCTGCCG AAGAAATCAT CGAACTGCCT GTAGATACCG TGGATGGAGT CGCCCATAGA
    AAACAATCAA TCAATTCATC GCCGCCAGCC AAGGGCGCGG GTGAATTCAA TCTGGAGGAG
    GAGAACTTCG CCCAGAGAGA TCATCACATC ATTGTCGAGG TGTTGATCTC CTCGTGGTAT
    GCCCTGTTGG CCAACACGGA TCTCATCTGC TACATTGTGG TTTTCATCAA TCAGGTGGTC
    AATGCCAGTC TCATTTCGCT GCCGCTGCCC ATTATGGTCT TTTTGTGGGG CACATTGTCT
    CTGCCGCGTC CCACTAAAAC CTTCTGGGTC ACGCTGATTG CCTACACCCA GGCCATCGTG
    CTGATCAAGT GCATCTTCCA GTTTAAGCTG ATCTGGTCCA ATTACCACCA ACTGCCCAAT
    CAGCCGCTAA CACCGGCCAA AATATTCGGC GTTGAGAATA AGGCCCATTA TGCGATTTAC
    GACCTGATAC TATTGCTGGT TCTATTCCTG CATCGCTATT TGCTTAAATC ACAAGGCCTG
    TGGAAATCGG GCTACAAGGA CACGGATAAC CAGTTCACCA AGCCCACCGC TAGCATTGAT
    GAACGCGACG ATAGCGACAA CTTATCCCAA CCGGATTCCC GCCAGCTAAA CGATGATGCT
    GCTCAGAAGC TGAGTCTCCA AGTGAGCCAG GCTTCCTTGC CAGGATCGCC CGAGTTTAGC
    AAGACAGGCA TCAATCAGCT AGAGCGCACC AAGTACACTT CATCGCTGTA CAAATTCTTC
    TTCAGTTTGG TCCACAAATC CCGTCTAGCC ACAGATGTTT ACGCTCTGAT GTTCCTCTGC
    GATTTTGTGA ACTTCTTCGT GCTTCTTTTC GGCTTCACTG CTTTTGGAAC TCAGCAAACG
    GAAAGTGATG AAGGTGTGCA GACATATCTT GCGGAGAACA AAGTGCCCAT ACCATTCCTG
    ATTATGCTAC TTGTTCAGTT CCTGCTCATT GTCATTGATC GAGCTTTGTA TCTGCGCAAA
    GCCCTGGTGA ACAAGATCAT CTTCCACTTC TTTTCGGTTA TCGGAATACA CATCTGGATG
    TTCTTCGTTG TGCCTGCAGT TACGGAGCGC ACTTTCAACT CCCTCGCACC TCCAATAATA
    TTCTACGTTA TCAAGTGCTT TTACATGCTG CTCAGCTCTT ATCAAATCAA ATCCGGTTAC
    CCTAAGCGCA TTCTAGGCAA CTTCTTCACC AAGGGTTTCT CGATGGTCAA TATGATTGCC
    TTTAAGGTGT ACATGCAGAT TCCATTCCTG TACGAGCTGC GAACCATATT AGATTGGGTG
    TGCATTGACA GCACCATGAC CATTTTCGAT TGGCTAAAGA TGGAGGACAT TTTCTCGAAT
    ATATATCTTA TCCGCTGCAC CCGGCAGTCA GAGACTGATT TTCCGGCCAT GAGAGCTCAG
    AAGAAGGCCT CACTTTCAAA GCTGATAATG GGTGGCACCA TCGTCCTGCT GATAGTGATT
    TGCATTTGGG GACCATTGTG TTTGTTTGCA CTTGGCAACG CGGTGGGCAC CTCGAATGTG
    CCGTTCCATG TGTCGCTATC AATACGAATT GGACCCTATG ATCCCATCTA CACAACGAAC
    AACTACGACA GTATCTTCGA GATCAATCCT GAGATGTACT CTCAGATGAC TAATGCATAC
    ATAAAGGAGA AACAGGCTTT GACGTTTATT GCCGGCTATG ATGCAACGGA TGTAGCGGCG
    GTTAGACTAG CTGGCAACTC GCCATCTCTG TGGAACATAG CACCGCCGGA TAGGCAGCGA
    CTACTAAATG ATTTAAGAAA CAATCACACG CTGAAGGCCC GGTTCTCTTA TTCCCTCACC
    CGAAAGGCTC CTGCCAAGGG GCTAAAGGAA AATGTGGGCG ACGAGCATGC CATATCGCTG
    GACGAGTCCT TTGAGGGACG AGCAGCACTC ATTCACATGC TAAGCGAAAC CCATGATGTG
    GAGCCCATTT ACAGCAATGG CACCACAAAT GGAACCACTC CCGAAGTTGA AGAAGTGGTG
    GTGATACCCG GAATGATACC CAAGTTTATC AAGGTTCTCA ATTCGGGCGA CGCTGCGGTG
    GTAAGTGTTT TGAGCCCAAA ACACTACGAC TACCGACCGT TGGTTATCAA AATGCATCGG
    GACAACGAGA CCAATGGGTT GTGGTGGGAG ATTCGGGACT ATTGCAACGA CACCTTCTAC
    AACGAAACTC TATCGAAGTT TGCATACAGC AACTGCACTT CGGGAATTGT GATGTACACG
    TTCAACGATA AAAAGTTTCC ATCCACGTTC AGTTTCCTCA CAGCGGGAGG CATCATTGGT
    CTGTACACTA CCTTTGTGTT ATTGGCCTCG CGCTTTATGA AGTCCTTCAT TGGCGGGCAA
    AACAGAAAGA TCATGTTTGA GGATCTGCCC TATGTTGATA GAGTGCTGCA GCTCTGTCTG
    GACATTTATC TGGTACGGGA GGCATTGGAA TTCGCTTTGG AAGAGGACCT GTTTGCCAAA
    TTACTCTTCC TATACCGATC GCCCGAAACG CTAATCAAGT GGACGCGTCC CAAGGAGGAG
    TACGTGGACG ATGATGGCGA CACCGACTCG ATTCCCAGTC GAATGAGCGT GCGCCGGCCG
    GAGCAACTGC AGCCACAGCA ACCGCAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa14090
    Clone ID Related Accession (Same CDS sequence) NM_001042881.5
    Accession Version NM_001042881.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8016bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product piezo, isoform F
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001042881.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGTCTTCA GCTATGCGTG CATGGTGCTC CAGCGCATCG TGGTGCCAGC GGTTCTGGTA 
    CTCGCTGCCC TGATGCGACC AGTGGGCATA TCCTTTGTCT ACCTTTTGAT GTTCTTTGTG
    TCGCCCTTTG TTCCGCTGGC CACGCGACGC AACTTCAAAG GATCTGTGAC TGCCTTCTTC
    ATCATCCTGC TGACGTTGAG CACGCTGGTC CTCTTGGGTC ACATAACGCT ACAGATTCTG
    GCGGTCAGCC TCACGCTGCC CATCTACAAT TGCTCGTTCA GTGAGCGTCT GCTGCGTCAC
    ATTGGCTTCG TGAGCTTTAT TGATCTACAG CCATTTGCCA TCATCGAATG GCTAGTGCCT
    GAGGTGTTGG TTTTCGCCAC CTCCCTGGGT TCGTATCTCA CGGTGAAGCG AGTGGCCTCA
    CAGCCCGTCG GCGCCGAGCA GCTGGAGAAC GGCGAGGTGG TCGATGGCCA GGCGGAAAAC
    GCGCAGACAT CTTCTCAGCC ATCTGCCGCA GATGCCAATG GAGGAGATGT GCAACAGGCC
    ACGGTCACCA CGCCGCTGCA GCAACAGCAG CAGCAGCTGA GGAAACGAGT GTCCATGATC
    AGCCAGCACA TACACTTTGA GGGCTTGGTC AAGATCTCTC CTCTGTTCTG CCTGGCCACG
    CTGTTCTTTG CGGCCGTACT GCGTCCCTCG GTGCCTGGCG GATTTTACTT TCTCATTTTC
    CTGCTGTCCG GCACCTACTG GGCAACATGC CAGACGCTGC AACGGGGCTT CGCTTTGTTG
    CTGCGCTGCG TGATGGTCGT CCTCGTGCTG CACTCCCTGT CCATTGTGTC ATACCAGACG
    CCATGGATGC AGAGCCACCT CAATCATACC ACCCTGACAG CTCGATTGAT TGGACTGGAA
    CCGCTTATTG AATCCTACTG CTCGCCGGAT ATACGAGTCT TTCTGTACAA TAATAAGCTA
    TCCCTGGACT CGTACCTCAA TCCCTTTGCG TTGTTCTTTG CCTATTTCGC ACTGGCACTG
    ACCACCAAGC ATCTGATTAA GCCACGGCTG GAGCCCAAGC CCGCCACCGC ACTTGGGCAG
    CAACTAGATT GCAATAGCAG CAGCATCAAC AACACCACCG GCAACAAGGT CAACCGCCAG
    CTATCGCTGC TCACCTCGCA GACATCGCGG GGTCGTCGGG ATGGGTCGAA TCCTGGCGGA
    GGTGGAACGA CCATTACCAC CACCACGACC ACCACCTCCA CCACTGCAAT CCGCAATCAG
    CGCTTGAGTT TGGTGCGGCA AAGCACGCGA AAGGCACGTA CACCCCAACC GCTGGAAAGT
    GGATCCTCGG TGGCGCCAAG TGTCACTCAG CGGGGCAACG ACATGCAGCT GGAATCGATG
    GAGCAGCGAT CGGAACAGGA GAACACCACC ACATCCATAC TGGATCAAAT ATCGTATGGA
    TTCGTCAGCG TGGGAGGATT CATATATCAG AATAGCTATA TATTCACCAA TATTCTTATG
    ATGGCCTGGT CCATAGTGTA TCACAGTTGG CTGACTTTTG TCCTCTTGCT GTCGGCCAAC
    GTACTGTGGA TGATTCCCAA CCAGAGGAAG GCCATGATGC GGTCCAGTCC CTTTATAGTT
    CTGTATGCTG AGGCCCTTTT GATTGCCCAA TACATATACG GCATGGATCT CAACAACGAA
    GAGCTGCCCA CGAGCGTTCC CACTGCGGGC ATTAACCTGC AACAAATTGG CTTCGAACGA
    CCCATCGAGA ACCAAATGCG GCCATGTGTG CCGCTGATCG TGAAGACAGC GTTTGTGCTA
    ATGTTTTGGG TGACATCACG ACAGTTCTTC AAGGAGAAGC GCGATCGCCG ACGGGACAGC
    ACTCTGGCGG ACTTCATTGC CCCACTGCAG ATTACTGTGG GATCGGCTGG CTCCAGCTAC
    CTCATCAACG ATGGCAAGAA GACCTCAAAG TTCCTAAAGA AGGCCGGCGA TGTGATCAAA
    AATCTATTGG TGCGTCTGTG GATCTGGCTA CTCGTGCTGG TTATCTTCCT TTGCGCCATC
    ACTGGCGAGA ACATGACCGG CTTCCGCATC TGCTACATGG CCCTGTTTCT ATTCTTCTTG
    CTAGTCTTTC AATCTTCGTC CAAGGCGTGG GTTAAGATCA TGTACGGCTT CTGGCTGTTT
    CTGATCTTCT ATGCCATGTC CATACTTATA TTGATCTACA CATATCAATT CGACAAGTTC
    GACACGTACT GGAGCGACTA TCTTAATGTG TCCGCGACTT TGCAAAAGGA CATCGGCCTT
    AAGCGCTATC AGACCAAGGA TCTGTTCCTC CATTTGGTCT CACCGACGAT AATTGTGATC
    CTGACCGTCA TACAAGTGCA CTACTTCCAC AAGCGCTTTA TTGCATCATT GCAGCAGCAG
    CCGTTGGCTG GCGGATCGGC ACAGCAGAAA CCCACGGAGA CAACTGCCTT GGAACCGGCG
    CCATCGAAGC GACGTGGCAG CGCCGGTTCA CTGCGTAAAT CTCAGGGTCC ATCGGCGGAG
    GCTGCTCCAG GAGCCACCAC CGATTTCGAG ACATCTGTGC GAGACTTGGT GCGCATATCG
    TTCCGCAAGA TCAAGAACAA GTCGGAGTAC ATCTTCAAGA ACTTCAAGGA TGTCTTCTGG
    CGCTTCCTGG AGCTGCACAT CATGAAGGCT GTGTATATCG CAGCCTTCGT GTGCAGTGTC
    AGCGAAGTCT GCGTACTGCA CATTATCTTT GTGGGTTTCT GTGTGCTGGG CGCCACCTCG
    CGGAAGGCCG TTCAGGTGGT GATCAGCCGC CTCATCTCGT TCATTGTCAC CGTCATAGTT
    CTGTCCAAGA TGATCTACCA GATCGAGTAC TTGAGTCACT CGCAGCACAA TGTGGTTTGT
    TCTGACAACC GGACTGCCAA CAATGCGGAG TGGATTGGTC TCACCAAGGC TGACAAGGTA
    ACTGGCGGAC TGATGAGCCT GTTGCGCACC TACATCATCT ACATGGTTAT TGTGACCATG
    CACGCAGTGA TCAGTTTGCG GCAGCTTCAA ATGCGCGTCA AGATCGGAGC ACTGAATGCT
    CCACCCACCA AGCTGCTGTT CCCCAATATT ATTCGAGCTG ATGCTGAGAA GGATCTGGTG
    GGACTGGTCA AGTATCTCCT CAACTTTGGC TTCTACAAAT TCGGCATTGA GATATCGCTA
    ATCGCGCTGG TCTCTACCAT CACATATCGC CAGGATATTG TTGCCGTAGT CTATGCTCTG
    TGGCTAGTGG TGCTTTTGCT TCTACGGAGA TCGCAGTGCG CCAAAATATG GGGCGTGTTT
    CAGGCATTCT TTGCCATCTC CATACTGACA CAGTATATAG TGCTGGTAGG ATTGCCGCCG
    AGCTCATGCC TGGTGTTTCC CTGGGATGAA GGTCCCTTCG GCGAGGGCAT ACAACGCTGG
    GCGATGCTGC CAGGAGCCCT GCACTTCAAC CACGTACCCA AGCTGATCTT CGACTTTATT
    GTCTTGGTCA TTCTGAATCG ACAGAAGAGT ATCTTCTGCA TCGAACAGCG TTATGCCAGT
    AACGATGACT ACCCGGGTGG CAGCAATCGC AGTGTGATCG CGGATATTGC TCAGCTAGGT
    CGCGTTCCCT TCGACAATCC CACCCACGAC TTTTGTTCGT ACATACGCAA CTACTCGGAC
    ATCCTTAAGA ACGGAGTGCT GTGCGGCTTC TACTGGTTTA CTCTGGCAGT TGTTTTCTTG
    GCCGGCACCA ATATTGCTGA TCTGCTGGCA CTGGGTTATC TGATCGGAGC GTTTATCTTC
    CTGTGGCAGG GATCGGATTT CTATCTGCGT CCCATACACA CCATCATCTT TCGCTGGAAG
    TGGCTGCTGG CATTCAATGT GGCCAACATA CTTATCAAGA CGTCCTTCCA AATGGCCGGC
    TGTTTGTTCA TGACCCAACT GACGAAAGAC TGCTGCTGGC TGGTGCACAT GCTCGGCATC
    ACATGTACGA GCAATGTACT TACAGAGCAA ATAATGCTGC CCGAGGAGGC TGAATTGGCG
    CTTAAGCCAG GCGAATGTCC TAAGATCACC CACCAGGTGG TCCTTCTGTG GGACACGATT
    TGCTTTGCCT TCATCATCTT CCAGCTGCGC ATCTTCAAGT CGCACTACTT CTGTCACATC
    ATAACGGACA CCAAAGCAAA TAACATCCTG GCCTCAAGAG GAGCCGACAT CATTGAGAGC
    CTACGACATA AGCAGATTGC ACATCGACAC GACCATGAAA AGCAGGTGCT GCATAAGATC
    AAGCGAAAGA TGGAGCGCAT CCGTGCCACG CAGCAGAAGA TGCTACGACC CTTGGACAAA
    CAAACCCACT TCGACGAACA TGGTTATCCA CTTCCTGCAC CAACAGTACG CAGAAGGAAG
    GAAATTAAAT TACATCCACA TGCTACCCGT GCTGGTGACT ACTATATGTT CGAGGAGATG
    GACGATAAGT TTGAGCTCGA CTTGATACAC GACGAGATCG ACTTCCTCGA GGAGGAGAAC
    ATCACCGAGA GCGAGATGAA GATGCAGCGA CGCAAGACCC TCTACGATGT GTGGAAGGAT
    CTGAATGATG CCGAGTATCG TCGCCACTTC TATATGCGTG AGCGTTCCTA CGCCTCAGAG
    CCCGGCTCAC GCATTCTCCT GAAGTTGGAC GACGAGAAGG AGAAGATTGG GCCCGATCTC
    GGCCTGGCCA GCGACCACGA TGAGGACGAC GATACCATTG AGTGCGACCT TGGCATGCGC
    ACCTCGACGC TGTCCGAACC GCTCGACTTT GGTCGTCGCA GCCAGACGCT GCTCGAGGTT
    AAGTCGAAGG ATGCACCCAC AGGCGAGTTT CCCTCCACCA GTAAGGGTAT TTCGAAGGAG
    CGCGATGCGG CAACGGCTTC CAGTTCGGCT AGTCCAGCGC CCACTAGGGA TGTGGGTGAT
    CTGCCTGTGA TTCCACCACC CTCCACTGGC CTGGGACGTG AGCAAACCTC CAAGGAGACC
    TCCGATAGCA AGTCCAAAAT GGAAGTGGAC AGCGGAGAGG TGACGGCCAA GGATTCGGAT
    GAGGACTTCG ATACCAATCC CATTATCAGA CTGCTCGAGG GCTTCTTGGT CACGCTGACC
    ATAAGACTGA ACCGCTTTTC GCGCAACTAT CGCTTTGTGA ATCGCATCCT GGCCGGCGAA
    AAGAAGACCC TAAAGGAGTC CAGTTCGTTG AACCGTCTGG GGCTGTCCAG TGCCGCTGCC
    ATGTTCCACT TCCTCAAATC GAATTTAGAG AGAAAACAAT CAATCAATTC ATCGCCGCCA
    GCCAAGGGCA CGATGCTCAG TCGAAAATCG GATTGTGGCC TGCCTGAGAT CCGTATTAAA
    GCGCCATCTA TCGAGCGCGG TGCACACTAT TACCATAATC ACCACAGCGG CGGTGGCTCA
    GGATCCTTGA GCAAACACTG GTCCTACGAG CAGGTGGACA GCGCGGGTGA ATTCAATCTG
    GAGGAGGAGA ACTTCGCCCA GAGAGATCAT CACATCATTG TCGAGGTGTT GATCTCCTCG
    TGGTATGCCC TGTTGGCCAA CACGGATCTC ATCTGCTACA TTGTGGTTTT CATCAATCAG
    GTGGTCAATG CCAGTCTCAT TTCGCTGCCG CTGCCCATTA TGGTCTTTTT GTGGGGCACA
    TTGTCTCTGC CGCGTCCCAC TAAAACCTTC TGGGTCACGC TGATTGCCTA CACCCAGGCC
    ATCGTGCTGA TCAAGTGCAT CTTCCAGTTT AAGCTGATCT GGTCCAATTA CCACCAACTG
    CCCAATCAGC CGCTAACACC GGCCAAAATA TTCGGCGTTG AGAATAAGGC CCATTATGCG
    ATTTACGACC TGATACTATT GCTGGTTCTA TTCCTGCATC GCTATTTGCT TAAATCACAA
    GGCCTGTGGA AATCGGGCTA CAAGGACACG GATAACCAGT TCACCAAGCC CACCGCTAGC
    ATTGATGAAC GCGACGATAG CGACAACTTA TCCCAACCGG ATTCCCGCCA GCTAAACGAT
    GATGCTGCTC AGAAGCTGAG TCTCCAAGTG AGCCAGGCTT CCTTGCCAGG ATCGCCCGAG
    TTTAGCAAGA CAGGCATCAA TCAGCTAGAG CGCACCAAGT ACACTTCATC GCTGTACAAA
    TTCTTCTTCA GTTTGGTCCA CAAATCCCGT CTAGCCACAG ATGTTTACGC TCTGATGTTC
    CTCTGCGATT TTGTGAACTT CTTCGTGCTT CTTTTCGGCT TCACTGCTTT TGGAACTCAG
    CAAACGGAAA GTGATGAAGG TGTGCAGACA TATCTTGCGG AGAACAAAGT GCCCATACCA
    TTCCTGATTA TGCTACTTGT TCAGTTCCTG CTCATTGTCA TTGATCGAGC TTTGTATCTG
    CGCAAAGCCC TGGTGAACAA GATCATCTTC CACTTCTTTT CGGTTATCGG AATACACATC
    TGGATGTTCT TCGTTGTGCC TGCAGTTACG GAGCGCACTT TCAACTCCCT CGCACCTCCA
    ATAATATTCT ACGTTATCAA GTGCTTTTAC ATGCTGCTCA GCTCTTATCA AATCAAATCC
    GGTTACCCTA AGCGCATTCT AGGCAACTTC TTCACCAAGG GTTTCTCGAT GGTCAATATG
    ATTGCCTTTA AGGTGTACAT GCAGATTCCA TTCCTGTACG AGCTGCGAAC CATATTAGAT
    TGGGTGTGCA TTGACAGCAC CATGACCATT TTCGATTGGC TAAAGATGGA GGACATTTTC
    TCGAATATAT ATCTTATCCG CTGCACCCGG CAGTCAGAGA CTGATTTTCC GGCCATGAGA
    GCTCAGAAGA AGGCCTCACT TTCAAAGCTG ATAATGGGTG GCACCATCGT CCTGCTGATA
    GTGATTTGCA TTTGGGGACC ATTGTGTTTG TTTGCACTTG GCAACGCGGT GGGCACCTCG
    AATGTGCCGT TCCATGTGTC GCTATCAATA CGAATTGGAC CCTATGATCC CATCTACACA
    ACGAACAACT ACGACAGTAT CTTCGAGATC AATCCTGAGA TGTACTCTCA GATGACTAAT
    GCATACATAA AGGAGAAACA GGCTTTGACG TTTATTGCCG GCTATGATGC AACGGATGTA
    GCGGCGGTTA GACTAGCTGG CAACTCGCCA TCTCTGTGGA ACATAGCACC GCCGGATAGG
    CAGCGACTAC TAAATGATTT AAGAAACAAT CACACGCTGA AGGCCCGGTT CTCTTATTCC
    CTCACCCGAA AGGCTCCTGC CAAGGGGCTA AAGGAAAATG TGGGCGACGA GCATGCCATA
    TCGCTGGACG AGTCCTTTGA GGGACGAGCA GCACTCATTC ACATGCTAAG CGAAACCCAT
    GATGTGGAGC CCATTTACAG CAATGGCACC ACAAATGGAA CCACTCCCGA AGTTGAAGAA
    GTGGTGGTGA TACCCGGAAT GATACCCAAG TTTATCAAGG TTCTCAATTC GGGCGACGCT
    GCGGTGGTAA GTGTTTTGAG CCCAAAACAC TACGACTACC GACCGTTGGT TATCAAAATG
    CATCGGGACA ACGAGACCAA TGGGTTGTGG TGGGAGATTC GGGACTATTG CAACGACACC
    TTCTACAACG AAACTCTATC GAAGTTTGCA TACAGCAACT GCACTTCGGG AATTGTGATG
    TACACGTTCA ACGATAAAAA GTTTCCATCC ACGTTCAGTT TCCTCACAGC GGGAGGCATC
    ATTGGTCTGT ACACTACCTT TGTGTTATTG GCCTCGCGCT TTATGAAGTC CTTCATTGGC
    GGGCAAAACA GAAAGATCAT GTTTGAGGAT CTGCCCTATG TTGATAGAGT GCTGCAGCTC
    TGTCTGGACA TTTATCTGGT ACGGGAGGCA TTGGAATTCG CTTTGGAAGA GGACCTGTTT
    GCCAAATTAC TCTTCCTATA CCGATCGCCC GAAACGCTAA TCAAGTGGAC GCGTCCCAAG
    GAGGAGTACG TGGACGATGA TGGCGACACC GACTCGATTC CCAGTCGAAT GAGCGTGCGC
    CGGCCGGAGC AACTGCAGCC ACAGCAACCG CAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001036346.3
    CDS577..8592
    Translation

    Target ORF information:

    RefSeq Version NM_001042881.5
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster piezo, transcript variant F (Piezo), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001042881.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGTCTTCA GCTATGCGTG CATGGTGCTC CAGCGCATCG TGGTGCCAGC GGTTCTGGTA 
    CTCGCTGCCC TGATGCGACC AGTGGGCATA TCCTTTGTCT ACCTTTTGAT GTTCTTTGTG
    TCGCCCTTTG TTCCGCTGGC CACGCGACGC AACTTCAAAG GATCTGTGAC TGCCTTCTTC
    ATCATCCTGC TGACGTTGAG CACGCTGGTC CTCTTGGGTC ACATAACGCT ACAGATTCTG
    GCGGTCAGCC TCACGCTGCC CATCTACAAT TGCTCGTTCA GTGAGCGTCT GCTGCGTCAC
    ATTGGCTTCG TGAGCTTTAT TGATCTACAG CCATTTGCCA TCATCGAATG GCTAGTGCCT
    GAGGTGTTGG TTTTCGCCAC CTCCCTGGGT TCGTATCTCA CGGTGAAGCG AGTGGCCTCA
    CAGCCCGTCG GCGCCGAGCA GCTGGAGAAC GGCGAGGTGG TCGATGGCCA GGCGGAAAAC
    GCGCAGACAT CTTCTCAGCC ATCTGCCGCA GATGCCAATG GAGGAGATGT GCAACAGGCC
    ACGGTCACCA CGCCGCTGCA GCAACAGCAG CAGCAGCTGA GGAAACGAGT GTCCATGATC
    AGCCAGCACA TACACTTTGA GGGCTTGGTC AAGATCTCTC CTCTGTTCTG CCTGGCCACG
    CTGTTCTTTG CGGCCGTACT GCGTCCCTCG GTGCCTGGCG GATTTTACTT TCTCATTTTC
    CTGCTGTCCG GCACCTACTG GGCAACATGC CAGACGCTGC AACGGGGCTT CGCTTTGTTG
    CTGCGCTGCG TGATGGTCGT CCTCGTGCTG CACTCCCTGT CCATTGTGTC ATACCAGACG
    CCATGGATGC AGAGCCACCT CAATCATACC ACCCTGACAG CTCGATTGAT TGGACTGGAA
    CCGCTTATTG AATCCTACTG CTCGCCGGAT ATACGAGTCT TTCTGTACAA TAATAAGCTA
    TCCCTGGACT CGTACCTCAA TCCCTTTGCG TTGTTCTTTG CCTATTTCGC ACTGGCACTG
    ACCACCAAGC ATCTGATTAA GCCACGGCTG GAGCCCAAGC CCGCCACCGC ACTTGGGCAG
    CAACTAGATT GCAATAGCAG CAGCATCAAC AACACCACCG GCAACAAGGT CAACCGCCAG
    CTATCGCTGC TCACCTCGCA GACATCGCGG GGTCGTCGGG ATGGGTCGAA TCCTGGCGGA
    GGTGGAACGA CCATTACCAC CACCACGACC ACCACCTCCA CCACTGCAAT CCGCAATCAG
    CGCTTGAGTT TGGTGCGGCA AAGCACGCGA AAGGCACGTA CACCCCAACC GCTGGAAAGT
    GGATCCTCGG TGGCGCCAAG TGTCACTCAG CGGGGCAACG ACATGCAGCT GGAATCGATG
    GAGCAGCGAT CGGAACAGGA GAACACCACC ACATCCATAC TGGATCAAAT ATCGTATGGA
    TTCGTCAGCG TGGGAGGATT CATATATCAG AATAGCTATA TATTCACCAA TATTCTTATG
    ATGGCCTGGT CCATAGTGTA TCACAGTTGG CTGACTTTTG TCCTCTTGCT GTCGGCCAAC
    GTACTGTGGA TGATTCCCAA CCAGAGGAAG GCCATGATGC GGTCCAGTCC CTTTATAGTT
    CTGTATGCTG AGGCCCTTTT GATTGCCCAA TACATATACG GCATGGATCT CAACAACGAA
    GAGCTGCCCA CGAGCGTTCC CACTGCGGGC ATTAACCTGC AACAAATTGG CTTCGAACGA
    CCCATCGAGA ACCAAATGCG GCCATGTGTG CCGCTGATCG TGAAGACAGC GTTTGTGCTA
    ATGTTTTGGG TGACATCACG ACAGTTCTTC AAGGAGAAGC GCGATCGCCG ACGGGACAGC
    ACTCTGGCGG ACTTCATTGC CCCACTGCAG ATTACTGTGG GATCGGCTGG CTCCAGCTAC
    CTCATCAACG ATGGCAAGAA GACCTCAAAG TTCCTAAAGA AGGCCGGCGA TGTGATCAAA
    AATCTATTGG TGCGTCTGTG GATCTGGCTA CTCGTGCTGG TTATCTTCCT TTGCGCCATC
    ACTGGCGAGA ACATGACCGG CTTCCGCATC TGCTACATGG CCCTGTTTCT ATTCTTCTTG
    CTAGTCTTTC AATCTTCGTC CAAGGCGTGG GTTAAGATCA TGTACGGCTT CTGGCTGTTT
    CTGATCTTCT ATGCCATGTC CATACTTATA TTGATCTACA CATATCAATT CGACAAGTTC
    GACACGTACT GGAGCGACTA TCTTAATGTG TCCGCGACTT TGCAAAAGGA CATCGGCCTT
    AAGCGCTATC AGACCAAGGA TCTGTTCCTC CATTTGGTCT CACCGACGAT AATTGTGATC
    CTGACCGTCA TACAAGTGCA CTACTTCCAC AAGCGCTTTA TTGCATCATT GCAGCAGCAG
    CCGTTGGCTG GCGGATCGGC ACAGCAGAAA CCCACGGAGA CAACTGCCTT GGAACCGGCG
    CCATCGAAGC GACGTGGCAG CGCCGGTTCA CTGCGTAAAT CTCAGGGTCC ATCGGCGGAG
    GCTGCTCCAG GAGCCACCAC CGATTTCGAG ACATCTGTGC GAGACTTGGT GCGCATATCG
    TTCCGCAAGA TCAAGAACAA GTCGGAGTAC ATCTTCAAGA ACTTCAAGGA TGTCTTCTGG
    CGCTTCCTGG AGCTGCACAT CATGAAGGCT GTGTATATCG CAGCCTTCGT GTGCAGTGTC
    AGCGAAGTCT GCGTACTGCA CATTATCTTT GTGGGTTTCT GTGTGCTGGG CGCCACCTCG
    CGGAAGGCCG TTCAGGTGGT GATCAGCCGC CTCATCTCGT TCATTGTCAC CGTCATAGTT
    CTGTCCAAGA TGATCTACCA GATCGAGTAC TTGAGTCACT CGCAGCACAA TGTGGTTTGT
    TCTGACAACC GGACTGCCAA CAATGCGGAG TGGATTGGTC TCACCAAGGC TGACAAGGTA
    ACTGGCGGAC TGATGAGCCT GTTGCGCACC TACATCATCT ACATGGTTAT TGTGACCATG
    CACGCAGTGA TCAGTTTGCG GCAGCTTCAA ATGCGCGTCA AGATCGGAGC ACTGAATGCT
    CCACCCACCA AGCTGCTGTT CCCCAATATT ATTCGAGCTG ATGCTGAGAA GGATCTGGTG
    GGACTGGTCA AGTATCTCCT CAACTTTGGC TTCTACAAAT TCGGCATTGA GATATCGCTA
    ATCGCGCTGG TCTCTACCAT CACATATCGC CAGGATATTG TTGCCGTAGT CTATGCTCTG
    TGGCTAGTGG TGCTTTTGCT TCTACGGAGA TCGCAGTGCG CCAAAATATG GGGCGTGTTT
    CAGGCATTCT TTGCCATCTC CATACTGACA CAGTATATAG TGCTGGTAGG ATTGCCGCCG
    AGCTCATGCC TGGTGTTTCC CTGGGATGAA GGTCCCTTCG GCGAGGGCAT ACAACGCTGG
    GCGATGCTGC CAGGAGCCCT GCACTTCAAC CACGTACCCA AGCTGATCTT CGACTTTATT
    GTCTTGGTCA TTCTGAATCG ACAGAAGAGT ATCTTCTGCA TCGAACAGCG TTATGCCAGT
    AACGATGACT ACCCGGGTGG CAGCAATCGC AGTGTGATCG CGGATATTGC TCAGCTAGGT
    CGCGTTCCCT TCGACAATCC CACCCACGAC TTTTGTTCGT ACATACGCAA CTACTCGGAC
    ATCCTTAAGA ACGGAGTGCT GTGCGGCTTC TACTGGTTTA CTCTGGCAGT TGTTTTCTTG
    GCCGGCACCA ATATTGCTGA TCTGCTGGCA CTGGGTTATC TGATCGGAGC GTTTATCTTC
    CTGTGGCAGG GATCGGATTT CTATCTGCGT CCCATACACA CCATCATCTT TCGCTGGAAG
    TGGCTGCTGG CATTCAATGT GGCCAACATA CTTATCAAGA CGTCCTTCCA AATGGCCGGC
    TGTTTGTTCA TGACCCAACT GACGAAAGAC TGCTGCTGGC TGGTGCACAT GCTCGGCATC
    ACATGTACGA GCAATGTACT TACAGAGCAA ATAATGCTGC CCGAGGAGGC TGAATTGGCG
    CTTAAGCCAG GCGAATGTCC TAAGATCACC CACCAGGTGG TCCTTCTGTG GGACACGATT
    TGCTTTGCCT TCATCATCTT CCAGCTGCGC ATCTTCAAGT CGCACTACTT CTGTCACATC
    ATAACGGACA CCAAAGCAAA TAACATCCTG GCCTCAAGAG GAGCCGACAT CATTGAGAGC
    CTACGACATA AGCAGATTGC ACATCGACAC GACCATGAAA AGCAGGTGCT GCATAAGATC
    AAGCGAAAGA TGGAGCGCAT CCGTGCCACG CAGCAGAAGA TGCTACGACC CTTGGACAAA
    CAAACCCACT TCGACGAACA TGGTTATCCA CTTCCTGCAC CAACAGTACG CAGAAGGAAG
    GAAATTAAAT TACATCCACA TGCTACCCGT GCTGGTGACT ACTATATGTT CGAGGAGATG
    GACGATAAGT TTGAGCTCGA CTTGATACAC GACGAGATCG ACTTCCTCGA GGAGGAGAAC
    ATCACCGAGA GCGAGATGAA GATGCAGCGA CGCAAGACCC TCTACGATGT GTGGAAGGAT
    CTGAATGATG CCGAGTATCG TCGCCACTTC TATATGCGTG AGCGTTCCTA CGCCTCAGAG
    CCCGGCTCAC GCATTCTCCT GAAGTTGGAC GACGAGAAGG AGAAGATTGG GCCCGATCTC
    GGCCTGGCCA GCGACCACGA TGAGGACGAC GATACCATTG AGTGCGACCT TGGCATGCGC
    ACCTCGACGC TGTCCGAACC GCTCGACTTT GGTCGTCGCA GCCAGACGCT GCTCGAGGTT
    AAGTCGAAGG ATGCACCCAC AGGCGAGTTT CCCTCCACCA GTAAGGGTAT TTCGAAGGAG
    CGCGATGCGG CAACGGCTTC CAGTTCGGCT AGTCCAGCGC CCACTAGGGA TGTGGGTGAT
    CTGCCTGTGA TTCCACCACC CTCCACTGGC CTGGGACGTG AGCAAACCTC CAAGGAGACC
    TCCGATAGCA AGTCCAAAAT GGAAGTGGAC AGCGGAGAGG TGACGGCCAA GGATTCGGAT
    GAGGACTTCG ATACCAATCC CATTATCAGA CTGCTCGAGG GCTTCTTGGT CACGCTGACC
    ATAAGACTGA ACCGCTTTTC GCGCAACTAT CGCTTTGTGA ATCGCATCCT GGCCGGCGAA
    AAGAAGACCC TAAAGGAGTC CAGTTCGTTG AACCGTCTGG GGCTGTCCAG TGCCGCTGCC
    ATGTTCCACT TCCTCAAATC GAATTTAGAG AGAAAACAAT CAATCAATTC ATCGCCGCCA
    GCCAAGGGCA CGATGCTCAG TCGAAAATCG GATTGTGGCC TGCCTGAGAT CCGTATTAAA
    GCGCCATCTA TCGAGCGCGG TGCACACTAT TACCATAATC ACCACAGCGG CGGTGGCTCA
    GGATCCTTGA GCAAACACTG GTCCTACGAG CAGGTGGACA GCGCGGGTGA ATTCAATCTG
    GAGGAGGAGA ACTTCGCCCA GAGAGATCAT CACATCATTG TCGAGGTGTT GATCTCCTCG
    TGGTATGCCC TGTTGGCCAA CACGGATCTC ATCTGCTACA TTGTGGTTTT CATCAATCAG
    GTGGTCAATG CCAGTCTCAT TTCGCTGCCG CTGCCCATTA TGGTCTTTTT GTGGGGCACA
    TTGTCTCTGC CGCGTCCCAC TAAAACCTTC TGGGTCACGC TGATTGCCTA CACCCAGGCC
    ATCGTGCTGA TCAAGTGCAT CTTCCAGTTT AAGCTGATCT GGTCCAATTA CCACCAACTG
    CCCAATCAGC CGCTAACACC GGCCAAAATA TTCGGCGTTG AGAATAAGGC CCATTATGCG
    ATTTACGACC TGATACTATT GCTGGTTCTA TTCCTGCATC GCTATTTGCT TAAATCACAA
    GGCCTGTGGA AATCGGGCTA CAAGGACACG GATAACCAGT TCACCAAGCC CACCGCTAGC
    ATTGATGAAC GCGACGATAG CGACAACTTA TCCCAACCGG ATTCCCGCCA GCTAAACGAT
    GATGCTGCTC AGAAGCTGAG TCTCCAAGTG AGCCAGGCTT CCTTGCCAGG ATCGCCCGAG
    TTTAGCAAGA CAGGCATCAA TCAGCTAGAG CGCACCAAGT ACACTTCATC GCTGTACAAA
    TTCTTCTTCA GTTTGGTCCA CAAATCCCGT CTAGCCACAG ATGTTTACGC TCTGATGTTC
    CTCTGCGATT TTGTGAACTT CTTCGTGCTT CTTTTCGGCT TCACTGCTTT TGGAACTCAG
    CAAACGGAAA GTGATGAAGG TGTGCAGACA TATCTTGCGG AGAACAAAGT GCCCATACCA
    TTCCTGATTA TGCTACTTGT TCAGTTCCTG CTCATTGTCA TTGATCGAGC TTTGTATCTG
    CGCAAAGCCC TGGTGAACAA GATCATCTTC CACTTCTTTT CGGTTATCGG AATACACATC
    TGGATGTTCT TCGTTGTGCC TGCAGTTACG GAGCGCACTT TCAACTCCCT CGCACCTCCA
    ATAATATTCT ACGTTATCAA GTGCTTTTAC ATGCTGCTCA GCTCTTATCA AATCAAATCC
    GGTTACCCTA AGCGCATTCT AGGCAACTTC TTCACCAAGG GTTTCTCGAT GGTCAATATG
    ATTGCCTTTA AGGTGTACAT GCAGATTCCA TTCCTGTACG AGCTGCGAAC CATATTAGAT
    TGGGTGTGCA TTGACAGCAC CATGACCATT TTCGATTGGC TAAAGATGGA GGACATTTTC
    TCGAATATAT ATCTTATCCG CTGCACCCGG CAGTCAGAGA CTGATTTTCC GGCCATGAGA
    GCTCAGAAGA AGGCCTCACT TTCAAAGCTG ATAATGGGTG GCACCATCGT CCTGCTGATA
    GTGATTTGCA TTTGGGGACC ATTGTGTTTG TTTGCACTTG GCAACGCGGT GGGCACCTCG
    AATGTGCCGT TCCATGTGTC GCTATCAATA CGAATTGGAC CCTATGATCC CATCTACACA
    ACGAACAACT ACGACAGTAT CTTCGAGATC AATCCTGAGA TGTACTCTCA GATGACTAAT
    GCATACATAA AGGAGAAACA GGCTTTGACG TTTATTGCCG GCTATGATGC AACGGATGTA
    GCGGCGGTTA GACTAGCTGG CAACTCGCCA TCTCTGTGGA ACATAGCACC GCCGGATAGG
    CAGCGACTAC TAAATGATTT AAGAAACAAT CACACGCTGA AGGCCCGGTT CTCTTATTCC
    CTCACCCGAA AGGCTCCTGC CAAGGGGCTA AAGGAAAATG TGGGCGACGA GCATGCCATA
    TCGCTGGACG AGTCCTTTGA GGGACGAGCA GCACTCATTC ACATGCTAAG CGAAACCCAT
    GATGTGGAGC CCATTTACAG CAATGGCACC ACAAATGGAA CCACTCCCGA AGTTGAAGAA
    GTGGTGGTGA TACCCGGAAT GATACCCAAG TTTATCAAGG TTCTCAATTC GGGCGACGCT
    GCGGTGGTAA GTGTTTTGAG CCCAAAACAC TACGACTACC GACCGTTGGT TATCAAAATG
    CATCGGGACA ACGAGACCAA TGGGTTGTGG TGGGAGATTC GGGACTATTG CAACGACACC
    TTCTACAACG AAACTCTATC GAAGTTTGCA TACAGCAACT GCACTTCGGG AATTGTGATG
    TACACGTTCA ACGATAAAAA GTTTCCATCC ACGTTCAGTT TCCTCACAGC GGGAGGCATC
    ATTGGTCTGT ACACTACCTT TGTGTTATTG GCCTCGCGCT TTATGAAGTC CTTCATTGGC
    GGGCAAAACA GAAAGATCAT GTTTGAGGAT CTGCCCTATG TTGATAGAGT GCTGCAGCTC
    TGTCTGGACA TTTATCTGGT ACGGGAGGCA TTGGAATTCG CTTTGGAAGA GGACCTGTTT
    GCCAAATTAC TCTTCCTATA CCGATCGCCC GAAACGCTAA TCAAGTGGAC GCGTCCCAAG
    GAGGAGTACG TGGACGATGA TGGCGACACC GACTCGATTC CCAGTCGAAT GAGCGTGCGC
    CGGCCGGAGC AACTGCAGCC ACAGCAACCG CAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa15940
    Clone ID Related Accession (Same CDS sequence) NM_001169436.3
    Accession Version NM_001169436.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8061bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product piezo, isoform G
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001169436.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    ATGGTCTTCA GCTATGCGTG CATGGTGCTC CAGCGCATCG TGGTGCCAGC GGTTCTGGTA 
    CTCGCTGCCC TGATGCGACC AGTGGGCATA TCCTTTGTCT ACCTTTTGAT GTTCTTTGTG
    TCGCCCTTTG TTCCGCTGGC CACGCGACGC AACTTCAAAG GATCTGTGAC TGCCTTCTTC
    ATCATCCTGC TGACGTTGAG CACGCTGGTC CTCTTGGGTC ACATAACGCT ACAGATTCTG
    GCGGTCAGCC TCACGCTGCC CATCTACAAT TGCTCGTTCA GTGAGCGTCT GCTGCGTCAC
    ATTGGCTTCG TGAGCTTTAT TGATCTACAG CCATTTGCCA TCATCGAATG GCTAGTGCCT
    GAGGTGTTGG TTTTCGCCAC CTCCCTGGGT TCGTATCTCA CGGTGAAGCG AGTGGCCTCA
    CAGCCCGTCG GCGCCGAGCA GCTGGAGAAC GGCGAGGTGG TCGATGGCCA GGCGGAAAAC
    GCGCAGACAT CTTCTCAGCC ATCTGCCGCA GATGCCAATG GAGGAGATGT GCAACAGGCC
    ACGGTCACCA CGCCGCTGCA GCAACAGCAG CAGCAGCTGA GGAAACGAGT GTCCATGATC
    AGCCAGCACA TACACTTTGA GGGCTTGGTC AAGATCTCTC CTCTGTTCTG CCTGGCCACG
    CTGTTCTTTG CGGCCGTACT GCGTCCCTCG GTGCCTGGCG GATTTTACTT TCTCATTTTC
    CTGCTGTCCG GCACCTACTG GGCAACATGC CAGACGCTGC AACGGGGCTT CGCTTTGTTG
    CTGCGCTGCG TGATGGTCGT CCTCGTGCTG CACTCCCTGT CCATTGTGTC ATACCAGACG
    CCATGGATGC AGAGCCACCT CAATCATACC ACCCTGACAG CTCGATTGAT TGGACTGGAA
    CCGCTTATTG AATCCTACTG CTCGCCGGAT ATACGAGTCT TTCTGTACAA TAATAAGCTA
    TCCCTGGACT CGTACCTCAA TCCCTTTGCG TTGTTCTTTG CCTATTTCGC ACTGGCACTG
    ACCACCAAGC ATCTGATTAA GCCACGGTTG GTGCGGCAAA GCACGCGAAA GGCACGTACA
    CCCCAACCGC TGGAAAGTGG ATCCTCGGTG GCGCCAAGTG TCACTCAGCG GGGCAACGAC
    ATGCAGCTGG AATCGATGGA GCAGCGATCG GAACAGGAGA ACACCACCAC ATCCATACTG
    GATCAAATAT CGTATGGATT CGTCAGCGTG GGAGGATTCA TATATCAGAA TAGCTATATA
    TTCACCAATA TTCTTATGAT GGCCTGGTCC ATAGTGTATC ACAGTTGGCT GACTTTTGTC
    CTCTTGCTGT CGGCCAACGT ACTGTGGATG ATTCCCAACC AGAGGAAGGC CATGATGCGG
    TCCAGTCCCT TTATAGTTCT GTATGCTGAG GCCCTTTTGA TTGCCCAATA CATATACGGC
    ATGGATCTCA ACAACGAAGA GCTGCCCACG AGCGTTCCCA CTGCGGGCAT TAACCTGCAA
    CAAATTGGCT TCGAACGACC CATCGAGAAC CAAATGCGGC CATGTGTGCC GCTGATCGTG
    AAGACAGCGT TTGTGCTAAT GTTTTGGGTG ACATCACGAC AGTTCTTCAA GGAGAAGCGC
    GATCGCCGAC GGGACAGCAC TCTGGCGGAC TTCATTGCCC CACTGCAGAT TACTGTGGGA
    TCGGCTGGCT CCAGCTACCT CATCAACGAT GGCAAGAAGA CCTCAAAGTT CCTAAAGAAG
    GCCGGCGATG TGATCAAAAA TCTATTGGTG CGTCTGTGGA TCTGGCTACT CGTGCTGGTT
    ATCTTCCTTT GCGCCATCAC TGGCGAGAAC ATGACCGGCT TCCGCATCTG CTACATGGCC
    CTGTTTCTAT TCTTCTTGCT AGTCTTTCAA TCTTCGTCCA AGGCGTGGGT TAAGATCATG
    TACGGCTTCT GGCTGTTTCT GATCTTCTAT GCCATGTCCA TACTTATATT GATCTACACA
    TATCAATTCG ACAAGTTCGA CACGTACTGG AGCGACTATC TTAATGTGTC CGCGACTTTG
    CAAAAGGACA TCGGCCTTAA GCGCTATCAG ACCAAGGATC TGTTCCTCCA TTTGGTCTCA
    CCGACGATAA TTGTGATCCT GACCGTCATA CAAGTGCACT ACTTCCACAA GCGCTTTATT
    GCATCATTGC AGCAGCAGCC GTTGGCTGGC GGATCGGCAC AGCAGAAACC CACGGAGACA
    ACTGCCTTGG AACCGGCGCC ATCGAAGCGA CGTGGCAGCG CCGGTTCACT GCGTAAATCT
    CAGGGTCCAT CGGCGGAGGC TGCTCCAGGA GCCACCACCG ATTTCGAGAC ATCTGTGCGA
    GACTTGGTGC GCATATCGTT CCGCAAGATC AAGAACAAGT CGGAGTACAT CTTCAAGAAC
    TTCAAGGATG TCTTCTGGCG CTTCCTGGAG CTGCACATCA TGAAGGCTGT GTATATCGCA
    GCCTTCGTGT GCAGTGTCAG CGAAGTCTGC GTACTGCACA TTATCTTTGT GGGTTTCTGT
    GTGCTGGGCG CCACCTCGCG GAAGGCCGTT CAGGTGGTGA TCAGCCGCCT CATCTCGTTC
    ATTGTCACCG TCATAGTTCT GTCCAAGATG ATCTACCAGA TCGAGTACTT GAGTCACTCG
    CAGCACAATG TGGTTTGTTC TGACAACCGG ACTGCCAACA ATGCGGAGTG GATTGGTCTC
    ACCAAGGCTG ACAAGGTAAC TGGCGGACTG ATGAGCCTGT TGCGCACCTA CATCATCTAC
    ATGGTTATTG TGACCATGCA CGCAGTGATC AGTTTGCGGC AGCTTCAAAT GCGCGTCAAG
    ATCGGAGCAC TGAATGCTCC ACCCACCAAG CTGCTGTTCC CCAATATTAT TCGAGCTGAT
    GCTGAGAAGG ATCTGGTGGG ACTGGTCAAG TATCTCCTCA ACTTTGGCTT CTACAAATTC
    GGCATTGAGA TATCGCTAAT CGCGCTGGTC TCTACCATCA CATATCGCCA GGATATTGTT
    GCCGTAGTCT ATGCTCTGTG GCTAGTGGTG CTTTTGCTTC TACGGAGATC GCAGTGCGCC
    AAAATATGGG GCGTGTTTCA GGCATTCTTT GCCATCTCCA TACTGACACA GTATATAGTG
    CTGGTAGGAT TGCCGCCGAG CTCATGCCTG GTGTTTCCCT GGGATGAAGG TCCCTTCGGC
    GAGGGCATAC AACGCTGGGC GATGCTGCCA GGAGCCCTGC ACTTCAACCA CGTACCCAAG
    CTGATCTTCG ACTTTATTGT CTTGGTCATT CTGAATCGAC AGAAGAGTAT CTTCTGCATC
    GAACAGCGTT ATGCCAGTAA CGATGACTAC CCGGGTGGCA GCAATCGCAG TGTGATCGCG
    GATATTGCTC AGCTAGGTCG CGTTCCCTTC GACAATCCCA CCCACGACTT TTGTTCGTAC
    ATACGCAACT ACTCGGACAT CCTTAAGAAC GGAGTGCTGT GCGGCTTCTA CTGGTTTACT
    CTGGCAGTTG TTTTCTTGGC CGGCACCAAT ATTGCTGATC TGCTGGCACT GGGTTATCTG
    ATCGGAGCGT TTATCTTCCT GTGGCAGGGA TCGGATTTCT ATCTGCGTCC CATACACACC
    ATCATCTTTC GCTGGAAGTG GCTGCTGGCA TTCAATGTGG CCAACATACT TATCAAGACG
    TCCTTCCAAA TGGCCGGCTG TTTGTTCATG ACCCAACTGA CGAAAGACTG CTGCTGGCTG
    GTGCACATGC TCGGCATCAC ATGTACGAGC AATGTACTTA CAGAGCAAAT AATGCTGCCC
    GAGGAGGCTG AATTGGCGCT TAAGCCAGGC GAATGTCCTA AGATCACCCA CCAGGTGGTC
    CTTCTGTGGG ACACGATTTG CTTTGCCTTC ATCATCTTCC AGCTGCGCAT CTTCAAGTCG
    CACTACTTCT GTCACATCAT AACGGACACC AAAGCAAATA ACATCCTGGC CTCAAGAGGA
    GCCGACATCA TTGAGAGCCT ACGACATAAG CAGATTGCAC ATCGACACGA CCATGAAAAG
    CAGGTGCTGC ATAAGATCAA GCGAAAGATG GAGCGCATCC GTGCCACGCA GCAGAAGATG
    CTACGACCCT TGGACAAACA AACCCACTTC GACGAACATG GTTATCCACT TCCTGCACCA
    ACAGTACGCA GAAGGAAGGA AATTAAATTA CATCCACATG CTACCCGTGC TGGTGACTAC
    TATATGTTCG AGGAGATGGA CGATAAGTTT GAGCTCGACT TGATACACGA CGAGATCGAC
    TTCCTCGAGG AGGAGAACAT CACCGAGAGC GAGATGAAGA TGCAGCGACG CAAGACCCTC
    TACGATGTGT GGAAGGATCT GAATGATGCC GAGTATCGTC GCCACTTCTA TATGCGTGAG
    CGTTCCTACG CCTCAGAGCC CGGCTCACGC ATTCTCCTGA AGTTGGACGA CGAGAAGGAG
    AAGATTGGGC CCGATCTCGG CCTGGCCAGC GACCACGATG AGGACGACGA TACCATTGAG
    TGCGACCTTG GCATGCGCAC CTCGACGCTG TCCGAACCGC TCGACTTTGG TCGTCGCAGC
    CAGACGCTGC TCGAGGTTAA GTCGAAGGAT GCACCCACAG GCGAGTTTCC CTCCACCAGT
    AAGGGTATTT CGAAGGAGCG CGATGCGGCA ACGGCTTCCA GTTCGGCTAG TCCAGCGCCC
    ACTAGGGATG TGGGTGATCT GCCTGTGATT CCACCACCCT CCACTGGCCT GGGACGTGAG
    CAAACCTCCA AGGAGACCTC CGATAGCAAG TCCAAAATGG AAGTGGACAG CGGAGAGGTG
    ACGGCCAAGG ATTCGGATGA GGACTTCGAT ACCAATCCCA TTATCAGACT GCTCGAGGGC
    TTCTTGGTCA CGCTGACCAT AAGACTGAAC CGCTTTTCGC GCAACTATCG CTTTGTGAAT
    CGCATCCTGG CCGGCGAAAA GAAGACCCTA AAGGAGTCCA GTTCGTTGAA CCGTCTGGGG
    CTGTCCAGTG CCGCTGCCAT GTTCCACTTC CTCAAATCGA ATTTAGAGAG CGATGAAAGT
    GAACCGCCCG CCTCCTCATC CACCCCGCGG CGGGTGGTGA TCGCACCACC GAATGCCACC
    GAGCACTCAG ACCCCACCAG CACCACACTG AACACGAACA CGACAACCAC ACCGCTATCA
    CCACCAGAAC CACTGCAACC ACTGCAACCA CTGCAACCAA ATACAACCAG TACACCACAG
    CAACAGCATC AGCATATTCG CGCTGCCGAA GAAATCATCG AACTGCCTGT AGATACCGTG
    GATGGAGTCG CCCATAGAAA ACAATCAATC AATTCATCGC CGCCAGCCAA GGGCACGATG
    CTCAGTCGAA AATCGGATTG TGGCCTGCCT GAGATCCGTA TTAAAGCGCC ATCTATCGAG
    CGCGGTGCAC ACTATTACCA TAATCACCAC AGCGGCGGTG GCTCAGGATC CTTGAGCAAA
    CACTGGTCCT ACGAGCAGGT GGACAGCGCG GGTGAATTCA ATCTGGAGGA GGAGAACTTC
    GCCCAGAGAG ATCATCACAT CATTGTCGAG GTGTTGATCT CCTCGTGGTA TGCCCTGTTG
    GCCAACACGG ATCTCATCTG CTACATTGTG GTTTTCATCA ATCAGGTGGT CAATGCCAGT
    CTCATTTCGC TGCCGCTGCC CATTATGGTC TTTTTGTGGG GCACATTGTC TCTGCCGCGT
    CCCACTAAAA CCTTCTGGGT CACGCTGATT GCCTACACCC AGGCCATCGT GCTGATCAAG
    TGCATCTTCC AGTTTAAGCT GATCTGGTCC AATTACCACC AACTGCCCAA TCAGCCGCTA
    ACACCGGCCA AAATATTCGG CGTTGAGAAT AAGGCCCATT ATGCGATTTA CGACCTGATA
    CTATTGCTGG TTCTATTCCT GCATCGCTAT TTGCTTAAAT CACAAGGCCT GTGGAAATCG
    GGCTACAAGG ACACGGATAA CCAGTTCACC AAGCCCACCG CTAGCATTGA TGAACGCGAC
    GATAGCGACA ACTTATCCCA ACCGGATTCC CGCCAGCTAA ACGATGATGC TGCTCAGAAG
    CTGAGTCTCC AAGTGAGCCA GGCTTCCTTG CCAGGATCGC CCGAGTTTAG CAAGACAGGC
    ATCAATCAGC TAGAGCGCAC CAAGTACACT TCATCGCTGT ACAAATTCTT CTTCAGTTTG
    GTCCACAAAT CCCGTCTAGC CACAGATGTT TACGCTCTGA TGTTCCTCTG CGATTTTGTG
    AACTTCTTCG TGCTTCTTTT CGGCTTCACT GCTTTTGGAA CTCAGCAAAC GGAAAGTGAT
    GAAGGTGTGC AGACATATCT TGCGGAGAAC AAAGTGCCCA TACCATTCCT GATTATGCTA
    CTTGTTCAGT TCCTGCTCAT TGTCATTGAT CGAGCTTTGT ATCTGCGCAA AGCCCTGGTG
    AACAAGATCA TCTTCCACTT CTTTTCGGTT ATCGGAATAC ACATCTGGAT GTTCTTCGTT
    GTGCCTGCAG TTACGGAGCG CACTTTCAAC TCCCTCGCAC CTCCAATAAT ATTCTACGTT
    ATCAAGTGCT TTTACATGCT GCTCAGCTCT TATCAAATCA AATCCGGTTA CCCTAAGCGC
    ATTCTAGGCA ACTTCTTCAC CAAGGGTTTC TCGATGGTCA ATATGATTGC CTTTAAGGTG
    TACATGCAGA TTCCATTCCT GTACGAGCTG CGAACCATAT TAGATTGGGT GTGCATTGAC
    AGCACCATGA CCATTTTCGA TTGGCTAAAG ATGGAGGACA TTTTCTCGAA TATATATCTT
    ATCCGCTGCA CCCGGCAGTC AGAGACTGAT TTTCCGGCCA TGAGAGCTCA GAAGAAGGCC
    TCACTTTCAA AGCTGATAAT GGGTGGCACC ATCGTCCTGC TGATAGTGAT TTGCATTTGG
    GGACCATTGT GTTTGTTTGC ACTTGGCAAC GCGGTGGGCA CCTCGAATGT GCCGTTCCAT
    GTGTCGCTAT CAATACGAAT TGGACCCTAT GATCCCATCT ACACAACGAA CAACTACGAC
    AGTATCTTCG AGATCAATCC TGAGATGTAC TCTCAGATGA CTAATGCATA CATAAAGGAG
    AAACAGGCTT TGACGTTTAT TGCCGGCTAT GATGCAACGG ATGTAGCGGC GGTTAGACTA
    GCTGGCAACT CGCCATCTCT GTGGAACATA GCACCGCCGG ATAGGCAGCG ACTACTAAAT
    GATTTAAGAA ACAATCACAC GCTGAAGGCC CGGTTCTCTT ATTCCCTCAC CCGAAAGGCT
    CCTGCCAAGG GGCTAAAGGA AAATGTGGGC GACGAGCATG CCATATCGCT GGACGAGTCC
    TTTGAGGGAC GAGCAGCACT CATTCACATG CTAAGCGAAA CCCATGATGT GGAGCCCATT
    TACAGCAATG GCACCACAAA TGGAACCACT CCCGAAGTTG AAGAAGTGGT GGTGATACCC
    GGAATGATAC CCAAGTTTAT CAAGGTTCTC AATTCGGGCG ACGCTGCGGT GGTAAGTGTT
    TTGAGCCCAA AACACTACGA CTACCGACCG TTGGTTATCA AAATGCATCG GGACAACGAG
    ACCAATGGGT TGTGGTGGGA GATTCGGGAC TATTGCAACG ACACCTTCTA CAACGAAACT
    CTATCGAAGT TTGCATACAG CAACTGCACT TCGGGAATTG TGATGTACAC GTTCAACGAT
    AAAAAGTTTC CATCCACGTT CAGTTTCCTC ACAGCGGGAG GCATCATTGG TCTGTACACT
    ACCTTTGTGT TATTGGCCTC GCGCTTTATG AAGTCCTTCA TTGGCGGGCA AAACAGAAAG
    ATCATGTTTG AGGATCTGCC CTATGTTGAT AGAGTGCTGC AGCTCTGTCT GGACATTTAT
    CTGGTACGGG AGGCATTGGA ATTCGCTTTG GAAGAGGACC TGTTTGCCAA ATTACTCTTC
    CTATACCGAT CGCCCGAAAC GCTAATCAAG TGGACGCGTC CCAAGGAGGA GTACGTGGAC
    GATGATGGCG ACACCGACTC GATTCCCAGT CGAATGAGCG TGCGCCGGCC GGAGCAACTG
    CAGCCACAGC AACCGCAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001162907.1
    CDS577..8637
    Translation

    Target ORF information:

    RefSeq Version NM_001169436.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster piezo, transcript variant G (Piezo), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001169436.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    ATGGTCTTCA GCTATGCGTG CATGGTGCTC CAGCGCATCG TGGTGCCAGC GGTTCTGGTA 
    CTCGCTGCCC TGATGCGACC AGTGGGCATA TCCTTTGTCT ACCTTTTGAT GTTCTTTGTG
    TCGCCCTTTG TTCCGCTGGC CACGCGACGC AACTTCAAAG GATCTGTGAC TGCCTTCTTC
    ATCATCCTGC TGACGTTGAG CACGCTGGTC CTCTTGGGTC ACATAACGCT ACAGATTCTG
    GCGGTCAGCC TCACGCTGCC CATCTACAAT TGCTCGTTCA GTGAGCGTCT GCTGCGTCAC
    ATTGGCTTCG TGAGCTTTAT TGATCTACAG CCATTTGCCA TCATCGAATG GCTAGTGCCT
    GAGGTGTTGG TTTTCGCCAC CTCCCTGGGT TCGTATCTCA CGGTGAAGCG AGTGGCCTCA
    CAGCCCGTCG GCGCCGAGCA GCTGGAGAAC GGCGAGGTGG TCGATGGCCA GGCGGAAAAC
    GCGCAGACAT CTTCTCAGCC ATCTGCCGCA GATGCCAATG GAGGAGATGT GCAACAGGCC
    ACGGTCACCA CGCCGCTGCA GCAACAGCAG CAGCAGCTGA GGAAACGAGT GTCCATGATC
    AGCCAGCACA TACACTTTGA GGGCTTGGTC AAGATCTCTC CTCTGTTCTG CCTGGCCACG
    CTGTTCTTTG CGGCCGTACT GCGTCCCTCG GTGCCTGGCG GATTTTACTT TCTCATTTTC
    CTGCTGTCCG GCACCTACTG GGCAACATGC CAGACGCTGC AACGGGGCTT CGCTTTGTTG
    CTGCGCTGCG TGATGGTCGT CCTCGTGCTG CACTCCCTGT CCATTGTGTC ATACCAGACG
    CCATGGATGC AGAGCCACCT CAATCATACC ACCCTGACAG CTCGATTGAT TGGACTGGAA
    CCGCTTATTG AATCCTACTG CTCGCCGGAT ATACGAGTCT TTCTGTACAA TAATAAGCTA
    TCCCTGGACT CGTACCTCAA TCCCTTTGCG TTGTTCTTTG CCTATTTCGC ACTGGCACTG
    ACCACCAAGC ATCTGATTAA GCCACGGTTG GTGCGGCAAA GCACGCGAAA GGCACGTACA
    CCCCAACCGC TGGAAAGTGG ATCCTCGGTG GCGCCAAGTG TCACTCAGCG GGGCAACGAC
    ATGCAGCTGG AATCGATGGA GCAGCGATCG GAACAGGAGA ACACCACCAC ATCCATACTG
    GATCAAATAT CGTATGGATT CGTCAGCGTG GGAGGATTCA TATATCAGAA TAGCTATATA
    TTCACCAATA TTCTTATGAT GGCCTGGTCC ATAGTGTATC ACAGTTGGCT GACTTTTGTC
    CTCTTGCTGT CGGCCAACGT ACTGTGGATG ATTCCCAACC AGAGGAAGGC CATGATGCGG
    TCCAGTCCCT TTATAGTTCT GTATGCTGAG GCCCTTTTGA TTGCCCAATA CATATACGGC
    ATGGATCTCA ACAACGAAGA GCTGCCCACG AGCGTTCCCA CTGCGGGCAT TAACCTGCAA
    CAAATTGGCT TCGAACGACC CATCGAGAAC CAAATGCGGC CATGTGTGCC GCTGATCGTG
    AAGACAGCGT TTGTGCTAAT GTTTTGGGTG ACATCACGAC AGTTCTTCAA GGAGAAGCGC
    GATCGCCGAC GGGACAGCAC TCTGGCGGAC TTCATTGCCC CACTGCAGAT TACTGTGGGA
    TCGGCTGGCT CCAGCTACCT CATCAACGAT GGCAAGAAGA CCTCAAAGTT CCTAAAGAAG
    GCCGGCGATG TGATCAAAAA TCTATTGGTG CGTCTGTGGA TCTGGCTACT CGTGCTGGTT
    ATCTTCCTTT GCGCCATCAC TGGCGAGAAC ATGACCGGCT TCCGCATCTG CTACATGGCC
    CTGTTTCTAT TCTTCTTGCT AGTCTTTCAA TCTTCGTCCA AGGCGTGGGT TAAGATCATG
    TACGGCTTCT GGCTGTTTCT GATCTTCTAT GCCATGTCCA TACTTATATT GATCTACACA
    TATCAATTCG ACAAGTTCGA CACGTACTGG AGCGACTATC TTAATGTGTC CGCGACTTTG
    CAAAAGGACA TCGGCCTTAA GCGCTATCAG ACCAAGGATC TGTTCCTCCA TTTGGTCTCA
    CCGACGATAA TTGTGATCCT GACCGTCATA CAAGTGCACT ACTTCCACAA GCGCTTTATT
    GCATCATTGC AGCAGCAGCC GTTGGCTGGC GGATCGGCAC AGCAGAAACC CACGGAGACA
    ACTGCCTTGG AACCGGCGCC ATCGAAGCGA CGTGGCAGCG CCGGTTCACT GCGTAAATCT
    CAGGGTCCAT CGGCGGAGGC TGCTCCAGGA GCCACCACCG ATTTCGAGAC ATCTGTGCGA
    GACTTGGTGC GCATATCGTT CCGCAAGATC AAGAACAAGT CGGAGTACAT CTTCAAGAAC
    TTCAAGGATG TCTTCTGGCG CTTCCTGGAG CTGCACATCA TGAAGGCTGT GTATATCGCA
    GCCTTCGTGT GCAGTGTCAG CGAAGTCTGC GTACTGCACA TTATCTTTGT GGGTTTCTGT
    GTGCTGGGCG CCACCTCGCG GAAGGCCGTT CAGGTGGTGA TCAGCCGCCT CATCTCGTTC
    ATTGTCACCG TCATAGTTCT GTCCAAGATG ATCTACCAGA TCGAGTACTT GAGTCACTCG
    CAGCACAATG TGGTTTGTTC TGACAACCGG ACTGCCAACA ATGCGGAGTG GATTGGTCTC
    ACCAAGGCTG ACAAGGTAAC TGGCGGACTG ATGAGCCTGT TGCGCACCTA CATCATCTAC
    ATGGTTATTG TGACCATGCA CGCAGTGATC AGTTTGCGGC AGCTTCAAAT GCGCGTCAAG
    ATCGGAGCAC TGAATGCTCC ACCCACCAAG CTGCTGTTCC CCAATATTAT TCGAGCTGAT
    GCTGAGAAGG ATCTGGTGGG ACTGGTCAAG TATCTCCTCA ACTTTGGCTT CTACAAATTC
    GGCATTGAGA TATCGCTAAT CGCGCTGGTC TCTACCATCA CATATCGCCA GGATATTGTT
    GCCGTAGTCT ATGCTCTGTG GCTAGTGGTG CTTTTGCTTC TACGGAGATC GCAGTGCGCC
    AAAATATGGG GCGTGTTTCA GGCATTCTTT GCCATCTCCA TACTGACACA GTATATAGTG
    CTGGTAGGAT TGCCGCCGAG CTCATGCCTG GTGTTTCCCT GGGATGAAGG TCCCTTCGGC
    GAGGGCATAC AACGCTGGGC GATGCTGCCA GGAGCCCTGC ACTTCAACCA CGTACCCAAG
    CTGATCTTCG ACTTTATTGT CTTGGTCATT CTGAATCGAC AGAAGAGTAT CTTCTGCATC
    GAACAGCGTT ATGCCAGTAA CGATGACTAC CCGGGTGGCA GCAATCGCAG TGTGATCGCG
    GATATTGCTC AGCTAGGTCG CGTTCCCTTC GACAATCCCA CCCACGACTT TTGTTCGTAC
    ATACGCAACT ACTCGGACAT CCTTAAGAAC GGAGTGCTGT GCGGCTTCTA CTGGTTTACT
    CTGGCAGTTG TTTTCTTGGC CGGCACCAAT ATTGCTGATC TGCTGGCACT GGGTTATCTG
    ATCGGAGCGT TTATCTTCCT GTGGCAGGGA TCGGATTTCT ATCTGCGTCC CATACACACC
    ATCATCTTTC GCTGGAAGTG GCTGCTGGCA TTCAATGTGG CCAACATACT TATCAAGACG
    TCCTTCCAAA TGGCCGGCTG TTTGTTCATG ACCCAACTGA CGAAAGACTG CTGCTGGCTG
    GTGCACATGC TCGGCATCAC ATGTACGAGC AATGTACTTA CAGAGCAAAT AATGCTGCCC
    GAGGAGGCTG AATTGGCGCT TAAGCCAGGC GAATGTCCTA AGATCACCCA CCAGGTGGTC
    CTTCTGTGGG ACACGATTTG CTTTGCCTTC ATCATCTTCC AGCTGCGCAT CTTCAAGTCG
    CACTACTTCT GTCACATCAT AACGGACACC AAAGCAAATA ACATCCTGGC CTCAAGAGGA
    GCCGACATCA TTGAGAGCCT ACGACATAAG CAGATTGCAC ATCGACACGA CCATGAAAAG
    CAGGTGCTGC ATAAGATCAA GCGAAAGATG GAGCGCATCC GTGCCACGCA GCAGAAGATG
    CTACGACCCT TGGACAAACA AACCCACTTC GACGAACATG GTTATCCACT TCCTGCACCA
    ACAGTACGCA GAAGGAAGGA AATTAAATTA CATCCACATG CTACCCGTGC TGGTGACTAC
    TATATGTTCG AGGAGATGGA CGATAAGTTT GAGCTCGACT TGATACACGA CGAGATCGAC
    TTCCTCGAGG AGGAGAACAT CACCGAGAGC GAGATGAAGA TGCAGCGACG CAAGACCCTC
    TACGATGTGT GGAAGGATCT GAATGATGCC GAGTATCGTC GCCACTTCTA TATGCGTGAG
    CGTTCCTACG CCTCAGAGCC CGGCTCACGC ATTCTCCTGA AGTTGGACGA CGAGAAGGAG
    AAGATTGGGC CCGATCTCGG CCTGGCCAGC GACCACGATG AGGACGACGA TACCATTGAG
    TGCGACCTTG GCATGCGCAC CTCGACGCTG TCCGAACCGC TCGACTTTGG TCGTCGCAGC
    CAGACGCTGC TCGAGGTTAA GTCGAAGGAT GCACCCACAG GCGAGTTTCC CTCCACCAGT
    AAGGGTATTT CGAAGGAGCG CGATGCGGCA ACGGCTTCCA GTTCGGCTAG TCCAGCGCCC
    ACTAGGGATG TGGGTGATCT GCCTGTGATT CCACCACCCT CCACTGGCCT GGGACGTGAG
    CAAACCTCCA AGGAGACCTC CGATAGCAAG TCCAAAATGG AAGTGGACAG CGGAGAGGTG
    ACGGCCAAGG ATTCGGATGA GGACTTCGAT ACCAATCCCA TTATCAGACT GCTCGAGGGC
    TTCTTGGTCA CGCTGACCAT AAGACTGAAC CGCTTTTCGC GCAACTATCG CTTTGTGAAT
    CGCATCCTGG CCGGCGAAAA GAAGACCCTA AAGGAGTCCA GTTCGTTGAA CCGTCTGGGG
    CTGTCCAGTG CCGCTGCCAT GTTCCACTTC CTCAAATCGA ATTTAGAGAG CGATGAAAGT
    GAACCGCCCG CCTCCTCATC CACCCCGCGG CGGGTGGTGA TCGCACCACC GAATGCCACC
    GAGCACTCAG ACCCCACCAG CACCACACTG AACACGAACA CGACAACCAC ACCGCTATCA
    CCACCAGAAC CACTGCAACC ACTGCAACCA CTGCAACCAA ATACAACCAG TACACCACAG
    CAACAGCATC AGCATATTCG CGCTGCCGAA GAAATCATCG AACTGCCTGT AGATACCGTG
    GATGGAGTCG CCCATAGAAA ACAATCAATC AATTCATCGC CGCCAGCCAA GGGCACGATG
    CTCAGTCGAA AATCGGATTG TGGCCTGCCT GAGATCCGTA TTAAAGCGCC ATCTATCGAG
    CGCGGTGCAC ACTATTACCA TAATCACCAC AGCGGCGGTG GCTCAGGATC CTTGAGCAAA
    CACTGGTCCT ACGAGCAGGT GGACAGCGCG GGTGAATTCA ATCTGGAGGA GGAGAACTTC
    GCCCAGAGAG ATCATCACAT CATTGTCGAG GTGTTGATCT CCTCGTGGTA TGCCCTGTTG
    GCCAACACGG ATCTCATCTG CTACATTGTG GTTTTCATCA ATCAGGTGGT CAATGCCAGT
    CTCATTTCGC TGCCGCTGCC CATTATGGTC TTTTTGTGGG GCACATTGTC TCTGCCGCGT
    CCCACTAAAA CCTTCTGGGT CACGCTGATT GCCTACACCC AGGCCATCGT GCTGATCAAG
    TGCATCTTCC AGTTTAAGCT GATCTGGTCC AATTACCACC AACTGCCCAA TCAGCCGCTA
    ACACCGGCCA AAATATTCGG CGTTGAGAAT AAGGCCCATT ATGCGATTTA CGACCTGATA
    CTATTGCTGG TTCTATTCCT GCATCGCTAT TTGCTTAAAT CACAAGGCCT GTGGAAATCG
    GGCTACAAGG ACACGGATAA CCAGTTCACC AAGCCCACCG CTAGCATTGA TGAACGCGAC
    GATAGCGACA ACTTATCCCA ACCGGATTCC CGCCAGCTAA ACGATGATGC TGCTCAGAAG
    CTGAGTCTCC AAGTGAGCCA GGCTTCCTTG CCAGGATCGC CCGAGTTTAG CAAGACAGGC
    ATCAATCAGC TAGAGCGCAC CAAGTACACT TCATCGCTGT ACAAATTCTT CTTCAGTTTG
    GTCCACAAAT CCCGTCTAGC CACAGATGTT TACGCTCTGA TGTTCCTCTG CGATTTTGTG
    AACTTCTTCG TGCTTCTTTT CGGCTTCACT GCTTTTGGAA CTCAGCAAAC GGAAAGTGAT
    GAAGGTGTGC AGACATATCT TGCGGAGAAC AAAGTGCCCA TACCATTCCT GATTATGCTA
    CTTGTTCAGT TCCTGCTCAT TGTCATTGAT CGAGCTTTGT ATCTGCGCAA AGCCCTGGTG
    AACAAGATCA TCTTCCACTT CTTTTCGGTT ATCGGAATAC ACATCTGGAT GTTCTTCGTT
    GTGCCTGCAG TTACGGAGCG CACTTTCAAC TCCCTCGCAC CTCCAATAAT ATTCTACGTT
    ATCAAGTGCT TTTACATGCT GCTCAGCTCT TATCAAATCA AATCCGGTTA CCCTAAGCGC
    ATTCTAGGCA ACTTCTTCAC CAAGGGTTTC TCGATGGTCA ATATGATTGC CTTTAAGGTG
    TACATGCAGA TTCCATTCCT GTACGAGCTG CGAACCATAT TAGATTGGGT GTGCATTGAC
    AGCACCATGA CCATTTTCGA TTGGCTAAAG ATGGAGGACA TTTTCTCGAA TATATATCTT
    ATCCGCTGCA CCCGGCAGTC AGAGACTGAT TTTCCGGCCA TGAGAGCTCA GAAGAAGGCC
    TCACTTTCAA AGCTGATAAT GGGTGGCACC ATCGTCCTGC TGATAGTGAT TTGCATTTGG
    GGACCATTGT GTTTGTTTGC ACTTGGCAAC GCGGTGGGCA CCTCGAATGT GCCGTTCCAT
    GTGTCGCTAT CAATACGAAT TGGACCCTAT GATCCCATCT ACACAACGAA CAACTACGAC
    AGTATCTTCG AGATCAATCC TGAGATGTAC TCTCAGATGA CTAATGCATA CATAAAGGAG
    AAACAGGCTT TGACGTTTAT TGCCGGCTAT GATGCAACGG ATGTAGCGGC GGTTAGACTA
    GCTGGCAACT CGCCATCTCT GTGGAACATA GCACCGCCGG ATAGGCAGCG ACTACTAAAT
    GATTTAAGAA ACAATCACAC GCTGAAGGCC CGGTTCTCTT ATTCCCTCAC CCGAAAGGCT
    CCTGCCAAGG GGCTAAAGGA AAATGTGGGC GACGAGCATG CCATATCGCT GGACGAGTCC
    TTTGAGGGAC GAGCAGCACT CATTCACATG CTAAGCGAAA CCCATGATGT GGAGCCCATT
    TACAGCAATG GCACCACAAA TGGAACCACT CCCGAAGTTG AAGAAGTGGT GGTGATACCC
    GGAATGATAC CCAAGTTTAT CAAGGTTCTC AATTCGGGCG ACGCTGCGGT GGTAAGTGTT
    TTGAGCCCAA AACACTACGA CTACCGACCG TTGGTTATCA AAATGCATCG GGACAACGAG
    ACCAATGGGT TGTGGTGGGA GATTCGGGAC TATTGCAACG ACACCTTCTA CAACGAAACT
    CTATCGAAGT TTGCATACAG CAACTGCACT TCGGGAATTG TGATGTACAC GTTCAACGAT
    AAAAAGTTTC CATCCACGTT CAGTTTCCTC ACAGCGGGAG GCATCATTGG TCTGTACACT
    ACCTTTGTGT TATTGGCCTC GCGCTTTATG AAGTCCTTCA TTGGCGGGCA AAACAGAAAG
    ATCATGTTTG AGGATCTGCC CTATGTTGAT AGAGTGCTGC AGCTCTGTCT GGACATTTAT
    CTGGTACGGG AGGCATTGGA ATTCGCTTTG GAAGAGGACC TGTTTGCCAA ATTACTCTTC
    CTATACCGAT CGCCCGAAAC GCTAATCAAG TGGACGCGTC CCAAGGAGGA GTACGTGGAC
    GATGATGGCG ACACCGACTC GATTCCCAGT CGAATGAGCG TGCGCCGGCC GGAGCAACTG
    CAGCCACAGC AACCGCAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa41330
    Clone ID Related Accession (Same CDS sequence) NM_001273300.2
    Accession Version NM_001273300.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8517bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product piezo, isoform O
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Oct 19, 2015 this sequence version replaced NM_001273300.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGGTCTTCA GCTATGCGTG CATGGTGCTC CAGCGCATCG TGGTGCCAGC GGTTCTGGTA 
    CTCGCTGCCC TGATGCGACC AGTGGGCATA TCCTTTGTCT ACCTTTTGAT GTTCTTTGTG
    TCGCCCTTTG TTCCGCTGGC CACGCGACGC AACTTCAAAG GATCTGTGAC TGCCTTCTTC
    ATCATCCTGC TGACGTTGAG CACGCTGGTC CTCTTGGGTC ACATAACGCT ACAGATTCTG
    GCGGTCAGCC TCACGCTGCC CATCTACAAT TGCTCGTTCA GTGAGCGTCT GCTGCGTCAC
    ATTGGCTTCG TGAGCTTTAT TGATCTACAG CCATTTGCCA TCATCGAATG GCTAGTGCCT
    GAGGTGTTGG TTTTCGCCAC CTCCCTGGGT TCGTATCTCA CGGTGAAGCG AGTGGCCTCA
    CAGCCCGTCG GCGCCGAGCA GCTGGAGAAC GGCGAGGTGG TCGATGGCCA GGCGGAAAAC
    GCGCAGACAT CTTCTCAGCC ATCTGCCGCA GATGCCAATG GAGGAGATGT GCAACAGGCC
    ACGGTCACCA CGCCGCTGCA GCAACAGCAG CAGCAGCTGA GGAAACGAGT GTCCATGATC
    AGCCAGCACA TACACTTTGA GGGCTTGGTC AAGATCTCTC CTCTGTTCTG CCTGGCCACG
    CTGTTCTTTG CGGCCGTACT GCGTCCCTCG GTGCCTGGCG GATTTTACTT TCTCATTTTC
    CTGCTGTCCG GCACCTACTG GGCAACATGC CAGACGCTGC AACGGGGCTT CGCTTTGTTG
    CTGCGCTGCG TGATGGTCGT CCTCGTGCTG CACTCCCTGT CCATTGTGTC ATACCAGACG
    CCATGGATGC AGAGCCACCT CAATCATACC ACCCTGACAG CTCGATTGAT TGGACTGGAA
    CCGCTTATTG AATCCTACTG CTCGCCGGAT ATACGAGTCT TTCTGTACAA TAATAAGCTA
    TCCCTGGACT CGTACCTCAA TCCCTTTGCG TTGTTCTTTG CCTATTTCGC ACTGGCACTG
    ACCACCAAGC ATCTGATTAA GCCACGGTTG GTGCGGCAAA GCACGCGAAA GGCACGTACA
    CCCCAACCGC TGGAAAGTGG ATCCTCGGTG GCGCCAAGTG TCACTCAGCG GGGCAACGAC
    ATGCAGCTGG AATCGATGGA GCAGCGATCG GAACAGGAGA ACACCACCAC ATCCATACTG
    GATCAAATAT CGTATGGATT CGTCAGCGTG GGAGGATTCA TATATCAGAA TAGCTATATA
    TTCACCAATA TTCTTATGAT GGCCTGGTCC ATAGTGTATC ACAGTTGGCT GACTTTTGTC
    CTCTTGCTGT CGGCCAACGT ACTGTGGATG ATTCCCAACC AGAGGAAGGC CATGATGCGG
    TCCAGTCCCT TTATAGTTCT GTATGCTGAG GCCCTTTTGA TTGCCCAATA CATATACGGC
    ATGGATCTCA ACAACGAAGA GCTGCCCACG AGCGTTCCCA CTGCGGGCAT TAACCTGCAA
    CAAATTGGCT TCGAACGACC CATCGAGAAC CAAATGCGGC CATGTGTGCC GCTGATCGTG
    AAGACAGCGT TTGTGCTAAT GTTTTGGGTG ACATCACGAC AGTTCTTCAA GGAGAAGCGC
    GATCGCCGAC GGGACAGCAC TCTGGCGGAC TTCATTGCCC CACTGCAGAT TACTGTGGGA
    TCGGCTGGCT CCAGCTACCT CATCAACGAT GGCAAGAAGA CCTCAAAGTT CCTAAAGAAG
    GCCGGCGATG TGATCAAAAA TCTATTGGTG CGTCTGTGGA TCTGGCTACT CGTGCTGGTT
    ATCTTCCTTT GCGCCATCAC TGGCGAGAAC ATGACCGGCT TCCGCATCTG CTACATGGCC
    CTGTTTCTAT TCTTCTTGCT AGTCTTTCAA TCTTCGTCCA AGGCGTGGGT TAAGATCATG
    TACGGCTTCT GGCTGTTTCT GATCTTCTAT GCCATGTCCA TACTTATATT GATCTACACA
    TATCAATTCG ACAAGTTCGA CACGTACTGG AGCGACTATC TTAATGTGTC CGCGACTTTG
    CAAAAGGACA TCGGCCTTAA GCGCTATCAG ACCAAGGATC TGTTCCTCCA TTTGGTCTCA
    CCGACGATAA TTGTGATCCT GACCGTCATA CAAGTGCACT ACTTCCACAA GCGCTTTATT
    GCATCATTGC AGCAGCAGCC GTTGGCTGGC GGATCGGCAC AGCAGAAACC CACGGAGACA
    ACTGCCTTGG AACCGGCGCC ATCGAAGCGA CGTGGCAGCG CCGGTTCACT GCGTAAATCT
    CAGGGTCCAT CGGCGGAGGC TGCTCCAGGA GCCACCACCG ATTTCGAGAC ATCTGTGCGA
    GACTTGGTGC GCATATCGTT CCGCAAGATC AAGAACAAGT CGGAGTACAT CTTCAAGAAC
    TTCAAGGATG TCTTCTGGCG CTTCCTGGAG CTGCACATCA TGAAGGCTGT GTATATCGCA
    GCCTTCGTGT GCAGTGTCAG CGAAGTCTGC GTACTGCACA TTATCTTTGT GGGTTTCTGT
    GTGCTGGGCG CCACCTCGCG GAAGGCCGTT CAGGTGGTGA TCAGCCGCCT CATCTCGTTC
    ATTGTCACCG TCATAGTTCT GTCCAAGATG ATCTACCAGA TCGAGTACTT GAGTCACTCG
    CAGCACAATG TGGTTTGTTC TGACAACCGG ACTGCCAACA ATGCGGAGTG GATTGGTCTC
    ACCAAGGCTG ACAAGGTAAC TGGCGGACTG ATGAGCCTGT TGCGCACCTA CATCATCTAC
    ATGGTTATTG TGACCATGCA CGCAGTGATC AGTTTGCGGC AGCTTCAAAT GCGCGTCAAG
    ATCGGAGCAC TGAATGCTCC ACCCACCAAG CTGCTGTTCC CCAATATTAT TCGAGCTGAT
    GCTGAGAAGG ATCTGGTGGG ACTGGTCAAG TATCTCCTCA ACTTTGGCTT CTACAAATTC
    GGCATTGAGA TATCGCTAAT CGCGCTGGTC TCTACCATCA CATATCGCCA GGATATTGTT
    GCCGTAGTCT ATGCTCTGTG GCTAGTGGTG CTTTTGCTTC TACGGAGATC GCAGTGCGCC
    AAAATATGGG GCGTGTTTCA GGCATTCTTT GCCATCTCCA TACTGACACA GTATATAGTG
    CTGGTAGGAT TGCCGCCGAG CTCATGCCTG GTGTTTCCCT GGGATGAAGG TCCCTTCGGC
    GAGGGCATAC AACGCTGGGC GATGCTGCCA GGAGCCCTGC ACTTCAACCA CGTACCCAAG
    CTGATCTTCG ACTTTATTGT CTTGGTCATT CTGAATCGAC AGAAGAGTAT CTTCTGCATC
    GAACAGCGTT ATGCCAGTAA CGATGACTAC CCGGGTGGCA GCAATCGCAG TGTGATCGCG
    GATATTGCTC AGCTAGGTCG CGTTCCCTTC GACAATCCCA CCCACGACTT TTGTTCGTAC
    ATACGCAACT ACTCGGACAT CCTTAAGAAC GGAGTGCTGT GCGGCTTCTA CTGGTTTACT
    CTGGCAGTTG TTTTCTTGGC CGGCACCAAT ATTGCTGATC TGCTGGCACT GGGTTATCTG
    ATCGGAGCGT TTATCTTCCT GTGGCAGGGA TCGGATTTCT ATCTGCGTCC CATACACACC
    ATCATCTTTC GCTGGAAGTG GCTGCTGGCA TTCAATGTGG CCAACATACT TATCAAGACG
    TCCTTCCAAA TGGCCGGCTG TTTGTTCATG ACCCAACTGA CGAAAGACTG CTGCTGGCTG
    GTGCACATGC TCGGCATCAC ATGTACGAGC AATGTACTTA CAGAGCAAAT AATGCTGCCC
    GAGGAGGCTG AATTGGCGCT TAAGCCAGGC GAATGTCCTA AGATCACCCA CCAGGTGGTC
    CTTCTGTGGG ACACGATTTG CTTTGCCTTC ATCATCTTCC AGCTGCGCAT CTTCAAGTCG
    CACTACTTCT GTCACATCAT AACGGACACC AAAGCAAATA ACATCCTGGC CTCAAGAGGA
    GCCGACATCA TTGAGAGCCT ACGACATAAG CAGATTGCAC ATCGACACGA CCATGAAAAG
    CAGGTGCTGC ATAAGATCAA GCGAAAGATG GAGCGCATCC GTGCCACGCA GCAGAAGATG
    CTACGACCCT TGGACAAACA AACCCACTTC GACGGGCGAC GCACGCCCTC AGAGCACGCC
    ATGTCCGATG TGGCGCCTTT GGTCCACAAT AGCAGCTTCA CCTCTTGTCA AGGATCTGGC
    TATCTGACCC CAGATGAGCA TAGCTCCGCC ACCTCAGGCA CTTCATCGCC AGCCAGGGCA
    TCGATCCTCT CCAGCAGTGG AGGCAGCGTG GAAAGCGTGG ACAGCGCAGA CGCTGCCGTG
    ATTATTGAAC CGGAAATCAA TGTGATGCCG TGTGATGAGA TTACGGACTA TGTGGATGTT
    CAGTCTGTCA GCGAGGAGGA GACAACTGTG GCAATGCCTA AAAAGGATCT TAGCTGCCAA
    TCAAAGGAAT GTGTTTTCAA AAGCCCAGAG CCTTCGAAGC CAACCACGCC GAGTACGGAT
    GCCTTGTCTA CTCTCCTGAC CGAGGGATTT TTGGAACCGA AAAGGATCTC GTTGGGATTG
    GCTCCTCCTG AGCTAAGTCC TCCTGTTGGG ATGCAGTGCC TGGCACCTCC GTTGGCTGCT
    TATGCCACAG CTATGGCTTC TAGTGCAGCC AGCTCTGTGC TGTCCACCAA TCTGACTCCA
    AATCTTCGCC GAGAACATCG GCGTCAGTCG TCAACCAATG CCGCTGTTTC CTGGAACGAA
    ACTGTATCCA TCAAACGATC GCCCATGAAA AAGCAAATGT CGGCGGATAA GGAGGAGGTG
    GTTACCATGC GGCACAAGTC GCTACACCGC CGACACCAGA GCATGGGAAA CGCCAGTTAC
    TCTTTGGACG AAGCCAGTCA GTCGCAGCGC AAGCGGTTGT CCAGCAACTT ATCCGGAGCT
    CGGGACGAGG CCGCTCTGCG ATCCGCACAG AGACCACATA GCTGGGGACC CGTGGGTACG
    AGCTCCTATA TGGAGTCGCT GATGTGCGCC TTCTACGAGC CTGCTCTGCT GCATGGACCA
    TCTACCCGTG CTGGTGACTA CTATATGTTC GAGGAGATGG ACGATAAGTT TGAGCTCGAC
    TTGATACACG ACGAGATCGA CTTCCTCGAG GAGGAGAACA TCACCGAGAG CGAGATGAAG
    ATGCAGCGAC GCAAGACCCT CTACGATAAG TCGAAGGATG CACCCACAGG CGAGTTTCCC
    TCCACCAGTA AGGGTATTTC GAAGGAGCGC GATGCGGCAA CGGCTTCCAG TTCGGCTAGT
    CCAGCGCCCA CTAGGGATGT GGGTGATCTG CCTGTGATTC CACCACCCTC CACTGGCCTG
    GGACGTGAGC AAACCTCCAA GGAGACCTCC GATAGCAAGT CCAAAATGGA AGTGGACAGC
    GGAGAGGTGA CGGCCAAGGA TTCGGATGAG GACTTCGATA CCAATCCCAT TATCAGACTG
    CTCGAGGGCT TCTTGGTCAC GCTGACCATA AGACTGAACC GCTTTTCGCG CAACTATCGC
    TTTGTGAATC GCATCCTGGC CGGCGAAAAG AAGACCCTAA AGGAGTCCAG TTCGTTGAAC
    CGTCTGGGGC TGTCCAGTGC CGCTGCCATG TTCCACTTCC TCAAATCGAA TTTAGAGAGC
    GATGAAAGTG AACCGCCCGC CTCCTCATCC ACCCCGCGGC GGGTGGTGAT CGCACCACCG
    AATGCCACCG AGCACTCAGA CCCCACCAGC ACCACACTGA ACACGAACAC GACAACCACA
    CCGCTATCAC CACCAGAACC ACTGCAACCA CTGCAACCAC TGCAACCAAA TACAACCAGT
    ACACCACAGC AACAGCATCA GCATATTCGC GCTGCCGAAG AAATCATCGA ACTGCCTGTA
    GATACCGTGG ATGGAGTCGC CCATAGAAAA CAATCAATCA ATTCATCGCC GCCAGCCAAG
    GGCGCGGGTG AATTCAATCT GGAGGAGGAG AACTTCGCCC AGAGAGATCA TCACATCATT
    GTCGAGGTGT TGATCTCCTC GTGGTATGCC CTGTTGGCCA ACACGGATCT CATCTGCTAC
    ATTGTGGTTT TCATCAATCA GGTGGTCAAT GCCAGTCTCA TTTCGCTGCC GCTGCCCATT
    ATGGTCTTTT TGTGGGGCAC ATTGTCTCTG CCGCGTCCCA CTAAAACCTT CTGGGTCACG
    CTGATTGCCT ACACCCAGGC CATCGTGCTG ATCAAGTGCA TCTTCCAGTT TAAGCTGATC
    TGGTCCAATT ACCACCAACT GCCCAATCAG CCGCTAACAC CGGCCAAAAT ATTCGGCGTT
    GAGAATAAGG CCCATTATGC GATTTACGAC CTGATACTAT TGCTGGTTCT ATTCCTGCAT
    CGCTATTTGC TTAAATCACA AGGCCTGTGG AAATCGGGCT ACAAGGACAC GGATAACCAG
    TTCACCAAGC CCACCGCTAG CATTGATGAA CGCGACGATA GCGACAACTT ATCCCAACCG
    GATTCCCGCC AGCTAAACGA TGATGCTGCT CAGAAGCTGA GTCTCCAAGT GAGCCAGGCT
    TCCTTGCCAG GATCGCCCGA GTTTAGCAAG ACAGGCATCA ATCAGCTAGA GCGCACCAAG
    TACACTTCAT CGCTGTACAA ATTCTTCTTC AGTTTGGTCC ACAAATCCCG TCTAGCCACA
    GATGTTTACG CTCTGATGTT CCTCTGCGAT TTTGTGAACT TCTTCGTGCT TCTTTTCGGC
    TTCACTGCTT TTGGAACTCA GCAAACGGAA AGTGATGAAG GTGTGCAGAC ATATCTTGCG
    GAGAACAAAG TGCCCATACC ATTCCTGATT ATGCTACTTG TTCAGTTCCT GCTCATTGTC
    ATTGATCGAG CTTTGTATCT GCGCAAAGCC CTGGTGAACA AGATCATCTT CCACTTCTTT
    TCGGTTATCG GAATACACAT CTGGATGTTC TTCGTTGTGC CTGCAGTTAC GGAGCGCACT
    TTCAACTCCC TCGCACCTCC AATAATATTC TACGTTATCA AGTGCTTTTA CATGCTGCTC
    AGCTCTTATC AAATCAAATC CGGTTACCCT AAGCGCATTC TAGGCAACTT CTTCACCAAG
    GGTTTCTCGA TGGTCAATAT GATTGCCTTT AAGGTGTACA TGCAGATTCC ATTCCTGTAC
    GAGCTGCGAA CCATATTAGA TTGGGTGTGC ATTGACAGCA CCATGACCAT TTTCGATTGG
    CTAAAGATGG AGGACATTTT CTCGAATATA TATCTTATCC GCTGCACCCG GCAGTCAGAG
    ACTGATTTTC CGGCCATGAG AGCTCAGAAG AAGGCCTCAC TTTCAAAGCT GATAATGGGT
    GGCACCATCG TCCTGCTGAT AGTGATTTGC ATTTGGGGAC CATTGTGTTT GTTTGCACTT
    GGCAACGCGG TGGGCACCTC GAATGTGCCG TTCCATGTGT CGCTATCAAT ACGAATTGGA
    CCCTATGATC CCATCTACAC AACGAACAAC TACGACAGTA TCTTCGAGAT CAATCCTGAG
    ATGTACTCTC AGATGACTAA TGCATACATA AAGGAGAAAC AGGCTTTGAC GTTTATTGCC
    GGCTATGATG CAACGGATGT AGCGGCGGTT AGACTAGCTG GCAACTCGCC ATCTCTGTGG
    AACATAGCAC CGCCGGATAG GCAGCGACTA CTAAATGATT TAAGAAACAA TCACACGCTG
    AAGGCCCGGT TCTCTTATTC CCTCACCCGA AAGGCTCCTG CCAAGGGGCT AAAGGAAAAT
    GTGGGCGACG AGCATGCCAT ATCGCTGGAC GAGTCCTTTG AGGGACGAGC AGCACTCATT
    CACATGCTAA GCGAAACCCA TGATGTGGAG CCCATTTACA GCAATGGCAC CACAAATGGA
    ACCACTCCCG AAGTTGAAGA AGTGGTGGTG ATACCCGGAA TGATACCCAA GTTTATCAAG
    GTTCTCAATT CGGGCGACGC TGCGGTGGTA AGTGTTTTGA GCCCAAAACA CTACGACTAC
    CGACCGTTGG TTATCAAAAT GCATCGGGAC AACGAGACCA ATGGGTTGTG GTGGGAGATT
    CGGGACTATT GCAACGACAC CTTCTACAAC GAAACTCTAT CGAAGTTTGC ATACAGCAAC
    TGCACTTCGG GAATTGTGAT GTACACGTTC AACGATAAAA AGTTTCCATC CACGTTCAGT
    TTCCTCACAG CGGGAGGCAT CATTGGTCTG TACACTACCT TTGTGTTATT GGCCTCGCGC
    TTTATGAAGT CCTTCATTGG CGGGCAAAAC AGAAAGATCA TGTTTGAGGA TCTGCCCTAT
    GTTGATAGAG TGCTGCAGCT CTGTCTGGAC ATTTATCTGG TACGGGAGGC ATTGGAATTC
    GCTTTGGAAG AGGACCTGTT TGCCAAATTA CTCTTCCTAT ACCGATCGCC CGAAACGCTA
    ATCAAGTGGA CGCGTCCCAA GGAGGAGTAC GTGGACGATG ATGGCGACAC CGACTCGATT
    CCCAGTCGAA TGAGCGTGCG CCGGCCGGAG CAACTGCAGC CACAGCAACC GCAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001260229.2
    CDS577..9093
    Translation

    Target ORF information:

    RefSeq Version NM_001273300.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster piezo, transcript variant O (Piezo), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001273300.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGGTCTTCA GCTATGCGTG CATGGTGCTC CAGCGCATCG TGGTGCCAGC GGTTCTGGTA 
    CTCGCTGCCC TGATGCGACC AGTGGGCATA TCCTTTGTCT ACCTTTTGAT GTTCTTTGTG
    TCGCCCTTTG TTCCGCTGGC CACGCGACGC AACTTCAAAG GATCTGTGAC TGCCTTCTTC
    ATCATCCTGC TGACGTTGAG CACGCTGGTC CTCTTGGGTC ACATAACGCT ACAGATTCTG
    GCGGTCAGCC TCACGCTGCC CATCTACAAT TGCTCGTTCA GTGAGCGTCT GCTGCGTCAC
    ATTGGCTTCG TGAGCTTTAT TGATCTACAG CCATTTGCCA TCATCGAATG GCTAGTGCCT
    GAGGTGTTGG TTTTCGCCAC CTCCCTGGGT TCGTATCTCA CGGTGAAGCG AGTGGCCTCA
    CAGCCCGTCG GCGCCGAGCA GCTGGAGAAC GGCGAGGTGG TCGATGGCCA GGCGGAAAAC
    GCGCAGACAT CTTCTCAGCC ATCTGCCGCA GATGCCAATG GAGGAGATGT GCAACAGGCC
    ACGGTCACCA CGCCGCTGCA GCAACAGCAG CAGCAGCTGA GGAAACGAGT GTCCATGATC
    AGCCAGCACA TACACTTTGA GGGCTTGGTC AAGATCTCTC CTCTGTTCTG CCTGGCCACG
    CTGTTCTTTG CGGCCGTACT GCGTCCCTCG GTGCCTGGCG GATTTTACTT TCTCATTTTC
    CTGCTGTCCG GCACCTACTG GGCAACATGC CAGACGCTGC AACGGGGCTT CGCTTTGTTG
    CTGCGCTGCG TGATGGTCGT CCTCGTGCTG CACTCCCTGT CCATTGTGTC ATACCAGACG
    CCATGGATGC AGAGCCACCT CAATCATACC ACCCTGACAG CTCGATTGAT TGGACTGGAA
    CCGCTTATTG AATCCTACTG CTCGCCGGAT ATACGAGTCT TTCTGTACAA TAATAAGCTA
    TCCCTGGACT CGTACCTCAA TCCCTTTGCG TTGTTCTTTG CCTATTTCGC ACTGGCACTG
    ACCACCAAGC ATCTGATTAA GCCACGGTTG GTGCGGCAAA GCACGCGAAA GGCACGTACA
    CCCCAACCGC TGGAAAGTGG ATCCTCGGTG GCGCCAAGTG TCACTCAGCG GGGCAACGAC
    ATGCAGCTGG AATCGATGGA GCAGCGATCG GAACAGGAGA ACACCACCAC ATCCATACTG
    GATCAAATAT CGTATGGATT CGTCAGCGTG GGAGGATTCA TATATCAGAA TAGCTATATA
    TTCACCAATA TTCTTATGAT GGCCTGGTCC ATAGTGTATC ACAGTTGGCT GACTTTTGTC
    CTCTTGCTGT CGGCCAACGT ACTGTGGATG ATTCCCAACC AGAGGAAGGC CATGATGCGG
    TCCAGTCCCT TTATAGTTCT GTATGCTGAG GCCCTTTTGA TTGCCCAATA CATATACGGC
    ATGGATCTCA ACAACGAAGA GCTGCCCACG AGCGTTCCCA CTGCGGGCAT TAACCTGCAA
    CAAATTGGCT TCGAACGACC CATCGAGAAC CAAATGCGGC CATGTGTGCC GCTGATCGTG
    AAGACAGCGT TTGTGCTAAT GTTTTGGGTG ACATCACGAC AGTTCTTCAA GGAGAAGCGC
    GATCGCCGAC GGGACAGCAC TCTGGCGGAC TTCATTGCCC CACTGCAGAT TACTGTGGGA
    TCGGCTGGCT CCAGCTACCT CATCAACGAT GGCAAGAAGA CCTCAAAGTT CCTAAAGAAG
    GCCGGCGATG TGATCAAAAA TCTATTGGTG CGTCTGTGGA TCTGGCTACT CGTGCTGGTT
    ATCTTCCTTT GCGCCATCAC TGGCGAGAAC ATGACCGGCT TCCGCATCTG CTACATGGCC
    CTGTTTCTAT TCTTCTTGCT AGTCTTTCAA TCTTCGTCCA AGGCGTGGGT TAAGATCATG
    TACGGCTTCT GGCTGTTTCT GATCTTCTAT GCCATGTCCA TACTTATATT GATCTACACA
    TATCAATTCG ACAAGTTCGA CACGTACTGG AGCGACTATC TTAATGTGTC CGCGACTTTG
    CAAAAGGACA TCGGCCTTAA GCGCTATCAG ACCAAGGATC TGTTCCTCCA TTTGGTCTCA
    CCGACGATAA TTGTGATCCT GACCGTCATA CAAGTGCACT ACTTCCACAA GCGCTTTATT
    GCATCATTGC AGCAGCAGCC GTTGGCTGGC GGATCGGCAC AGCAGAAACC CACGGAGACA
    ACTGCCTTGG AACCGGCGCC ATCGAAGCGA CGTGGCAGCG CCGGTTCACT GCGTAAATCT
    CAGGGTCCAT CGGCGGAGGC TGCTCCAGGA GCCACCACCG ATTTCGAGAC ATCTGTGCGA
    GACTTGGTGC GCATATCGTT CCGCAAGATC AAGAACAAGT CGGAGTACAT CTTCAAGAAC
    TTCAAGGATG TCTTCTGGCG CTTCCTGGAG CTGCACATCA TGAAGGCTGT GTATATCGCA
    GCCTTCGTGT GCAGTGTCAG CGAAGTCTGC GTACTGCACA TTATCTTTGT GGGTTTCTGT
    GTGCTGGGCG CCACCTCGCG GAAGGCCGTT CAGGTGGTGA TCAGCCGCCT CATCTCGTTC
    ATTGTCACCG TCATAGTTCT GTCCAAGATG ATCTACCAGA TCGAGTACTT GAGTCACTCG
    CAGCACAATG TGGTTTGTTC TGACAACCGG ACTGCCAACA ATGCGGAGTG GATTGGTCTC
    ACCAAGGCTG ACAAGGTAAC TGGCGGACTG ATGAGCCTGT TGCGCACCTA CATCATCTAC
    ATGGTTATTG TGACCATGCA CGCAGTGATC AGTTTGCGGC AGCTTCAAAT GCGCGTCAAG
    ATCGGAGCAC TGAATGCTCC ACCCACCAAG CTGCTGTTCC CCAATATTAT TCGAGCTGAT
    GCTGAGAAGG ATCTGGTGGG ACTGGTCAAG TATCTCCTCA ACTTTGGCTT CTACAAATTC
    GGCATTGAGA TATCGCTAAT CGCGCTGGTC TCTACCATCA CATATCGCCA GGATATTGTT
    GCCGTAGTCT ATGCTCTGTG GCTAGTGGTG CTTTTGCTTC TACGGAGATC GCAGTGCGCC
    AAAATATGGG GCGTGTTTCA GGCATTCTTT GCCATCTCCA TACTGACACA GTATATAGTG
    CTGGTAGGAT TGCCGCCGAG CTCATGCCTG GTGTTTCCCT GGGATGAAGG TCCCTTCGGC
    GAGGGCATAC AACGCTGGGC GATGCTGCCA GGAGCCCTGC ACTTCAACCA CGTACCCAAG
    CTGATCTTCG ACTTTATTGT CTTGGTCATT CTGAATCGAC AGAAGAGTAT CTTCTGCATC
    GAACAGCGTT ATGCCAGTAA CGATGACTAC CCGGGTGGCA GCAATCGCAG TGTGATCGCG
    GATATTGCTC AGCTAGGTCG CGTTCCCTTC GACAATCCCA CCCACGACTT TTGTTCGTAC
    ATACGCAACT ACTCGGACAT CCTTAAGAAC GGAGTGCTGT GCGGCTTCTA CTGGTTTACT
    CTGGCAGTTG TTTTCTTGGC CGGCACCAAT ATTGCTGATC TGCTGGCACT GGGTTATCTG
    ATCGGAGCGT TTATCTTCCT GTGGCAGGGA TCGGATTTCT ATCTGCGTCC CATACACACC
    ATCATCTTTC GCTGGAAGTG GCTGCTGGCA TTCAATGTGG CCAACATACT TATCAAGACG
    TCCTTCCAAA TGGCCGGCTG TTTGTTCATG ACCCAACTGA CGAAAGACTG CTGCTGGCTG
    GTGCACATGC TCGGCATCAC ATGTACGAGC AATGTACTTA CAGAGCAAAT AATGCTGCCC
    GAGGAGGCTG AATTGGCGCT TAAGCCAGGC GAATGTCCTA AGATCACCCA CCAGGTGGTC
    CTTCTGTGGG ACACGATTTG CTTTGCCTTC ATCATCTTCC AGCTGCGCAT CTTCAAGTCG
    CACTACTTCT GTCACATCAT AACGGACACC AAAGCAAATA ACATCCTGGC CTCAAGAGGA
    GCCGACATCA TTGAGAGCCT ACGACATAAG CAGATTGCAC ATCGACACGA CCATGAAAAG
    CAGGTGCTGC ATAAGATCAA GCGAAAGATG GAGCGCATCC GTGCCACGCA GCAGAAGATG
    CTACGACCCT TGGACAAACA AACCCACTTC GACGGGCGAC GCACGCCCTC AGAGCACGCC
    ATGTCCGATG TGGCGCCTTT GGTCCACAAT AGCAGCTTCA CCTCTTGTCA AGGATCTGGC
    TATCTGACCC CAGATGAGCA TAGCTCCGCC ACCTCAGGCA CTTCATCGCC AGCCAGGGCA
    TCGATCCTCT CCAGCAGTGG AGGCAGCGTG GAAAGCGTGG ACAGCGCAGA CGCTGCCGTG
    ATTATTGAAC CGGAAATCAA TGTGATGCCG TGTGATGAGA TTACGGACTA TGTGGATGTT
    CAGTCTGTCA GCGAGGAGGA GACAACTGTG GCAATGCCTA AAAAGGATCT TAGCTGCCAA
    TCAAAGGAAT GTGTTTTCAA AAGCCCAGAG CCTTCGAAGC CAACCACGCC GAGTACGGAT
    GCCTTGTCTA CTCTCCTGAC CGAGGGATTT TTGGAACCGA AAAGGATCTC GTTGGGATTG
    GCTCCTCCTG AGCTAAGTCC TCCTGTTGGG ATGCAGTGCC TGGCACCTCC GTTGGCTGCT
    TATGCCACAG CTATGGCTTC TAGTGCAGCC AGCTCTGTGC TGTCCACCAA TCTGACTCCA
    AATCTTCGCC GAGAACATCG GCGTCAGTCG TCAACCAATG CCGCTGTTTC CTGGAACGAA
    ACTGTATCCA TCAAACGATC GCCCATGAAA AAGCAAATGT CGGCGGATAA GGAGGAGGTG
    GTTACCATGC GGCACAAGTC GCTACACCGC CGACACCAGA GCATGGGAAA CGCCAGTTAC
    TCTTTGGACG AAGCCAGTCA GTCGCAGCGC AAGCGGTTGT CCAGCAACTT ATCCGGAGCT
    CGGGACGAGG CCGCTCTGCG ATCCGCACAG AGACCACATA GCTGGGGACC CGTGGGTACG
    AGCTCCTATA TGGAGTCGCT GATGTGCGCC TTCTACGAGC CTGCTCTGCT GCATGGACCA
    TCTACCCGTG CTGGTGACTA CTATATGTTC GAGGAGATGG ACGATAAGTT TGAGCTCGAC
    TTGATACACG ACGAGATCGA CTTCCTCGAG GAGGAGAACA TCACCGAGAG CGAGATGAAG
    ATGCAGCGAC GCAAGACCCT CTACGATAAG TCGAAGGATG CACCCACAGG CGAGTTTCCC
    TCCACCAGTA AGGGTATTTC GAAGGAGCGC GATGCGGCAA CGGCTTCCAG TTCGGCTAGT
    CCAGCGCCCA CTAGGGATGT GGGTGATCTG CCTGTGATTC CACCACCCTC CACTGGCCTG
    GGACGTGAGC AAACCTCCAA GGAGACCTCC GATAGCAAGT CCAAAATGGA AGTGGACAGC
    GGAGAGGTGA CGGCCAAGGA TTCGGATGAG GACTTCGATA CCAATCCCAT TATCAGACTG
    CTCGAGGGCT TCTTGGTCAC GCTGACCATA AGACTGAACC GCTTTTCGCG CAACTATCGC
    TTTGTGAATC GCATCCTGGC CGGCGAAAAG AAGACCCTAA AGGAGTCCAG TTCGTTGAAC
    CGTCTGGGGC TGTCCAGTGC CGCTGCCATG TTCCACTTCC TCAAATCGAA TTTAGAGAGC
    GATGAAAGTG AACCGCCCGC CTCCTCATCC ACCCCGCGGC GGGTGGTGAT CGCACCACCG
    AATGCCACCG AGCACTCAGA CCCCACCAGC ACCACACTGA ACACGAACAC GACAACCACA
    CCGCTATCAC CACCAGAACC ACTGCAACCA CTGCAACCAC TGCAACCAAA TACAACCAGT
    ACACCACAGC AACAGCATCA GCATATTCGC GCTGCCGAAG AAATCATCGA ACTGCCTGTA
    GATACCGTGG ATGGAGTCGC CCATAGAAAA CAATCAATCA ATTCATCGCC GCCAGCCAAG
    GGCGCGGGTG AATTCAATCT GGAGGAGGAG AACTTCGCCC AGAGAGATCA TCACATCATT
    GTCGAGGTGT TGATCTCCTC GTGGTATGCC CTGTTGGCCA ACACGGATCT CATCTGCTAC
    ATTGTGGTTT TCATCAATCA GGTGGTCAAT GCCAGTCTCA TTTCGCTGCC GCTGCCCATT
    ATGGTCTTTT TGTGGGGCAC ATTGTCTCTG CCGCGTCCCA CTAAAACCTT CTGGGTCACG
    CTGATTGCCT ACACCCAGGC CATCGTGCTG ATCAAGTGCA TCTTCCAGTT TAAGCTGATC
    TGGTCCAATT ACCACCAACT GCCCAATCAG CCGCTAACAC CGGCCAAAAT ATTCGGCGTT
    GAGAATAAGG CCCATTATGC GATTTACGAC CTGATACTAT TGCTGGTTCT ATTCCTGCAT
    CGCTATTTGC TTAAATCACA AGGCCTGTGG AAATCGGGCT ACAAGGACAC GGATAACCAG
    TTCACCAAGC CCACCGCTAG CATTGATGAA CGCGACGATA GCGACAACTT ATCCCAACCG
    GATTCCCGCC AGCTAAACGA TGATGCTGCT CAGAAGCTGA GTCTCCAAGT GAGCCAGGCT
    TCCTTGCCAG GATCGCCCGA GTTTAGCAAG ACAGGCATCA ATCAGCTAGA GCGCACCAAG
    TACACTTCAT CGCTGTACAA ATTCTTCTTC AGTTTGGTCC ACAAATCCCG TCTAGCCACA
    GATGTTTACG CTCTGATGTT CCTCTGCGAT TTTGTGAACT TCTTCGTGCT TCTTTTCGGC
    TTCACTGCTT TTGGAACTCA GCAAACGGAA AGTGATGAAG GTGTGCAGAC ATATCTTGCG
    GAGAACAAAG TGCCCATACC ATTCCTGATT ATGCTACTTG TTCAGTTCCT GCTCATTGTC
    ATTGATCGAG CTTTGTATCT GCGCAAAGCC CTGGTGAACA AGATCATCTT CCACTTCTTT
    TCGGTTATCG GAATACACAT CTGGATGTTC TTCGTTGTGC CTGCAGTTAC GGAGCGCACT
    TTCAACTCCC TCGCACCTCC AATAATATTC TACGTTATCA AGTGCTTTTA CATGCTGCTC
    AGCTCTTATC AAATCAAATC CGGTTACCCT AAGCGCATTC TAGGCAACTT CTTCACCAAG
    GGTTTCTCGA TGGTCAATAT GATTGCCTTT AAGGTGTACA TGCAGATTCC ATTCCTGTAC
    GAGCTGCGAA CCATATTAGA TTGGGTGTGC ATTGACAGCA CCATGACCAT TTTCGATTGG
    CTAAAGATGG AGGACATTTT CTCGAATATA TATCTTATCC GCTGCACCCG GCAGTCAGAG
    ACTGATTTTC CGGCCATGAG AGCTCAGAAG AAGGCCTCAC TTTCAAAGCT GATAATGGGT
    GGCACCATCG TCCTGCTGAT AGTGATTTGC ATTTGGGGAC CATTGTGTTT GTTTGCACTT
    GGCAACGCGG TGGGCACCTC GAATGTGCCG TTCCATGTGT CGCTATCAAT ACGAATTGGA
    CCCTATGATC CCATCTACAC AACGAACAAC TACGACAGTA TCTTCGAGAT CAATCCTGAG
    ATGTACTCTC AGATGACTAA TGCATACATA AAGGAGAAAC AGGCTTTGAC GTTTATTGCC
    GGCTATGATG CAACGGATGT AGCGGCGGTT AGACTAGCTG GCAACTCGCC ATCTCTGTGG
    AACATAGCAC CGCCGGATAG GCAGCGACTA CTAAATGATT TAAGAAACAA TCACACGCTG
    AAGGCCCGGT TCTCTTATTC CCTCACCCGA AAGGCTCCTG CCAAGGGGCT AAAGGAAAAT
    GTGGGCGACG AGCATGCCAT ATCGCTGGAC GAGTCCTTTG AGGGACGAGC AGCACTCATT
    CACATGCTAA GCGAAACCCA TGATGTGGAG CCCATTTACA GCAATGGCAC CACAAATGGA
    ACCACTCCCG AAGTTGAAGA AGTGGTGGTG ATACCCGGAA TGATACCCAA GTTTATCAAG
    GTTCTCAATT CGGGCGACGC TGCGGTGGTA AGTGTTTTGA GCCCAAAACA CTACGACTAC
    CGACCGTTGG TTATCAAAAT GCATCGGGAC AACGAGACCA ATGGGTTGTG GTGGGAGATT
    CGGGACTATT GCAACGACAC CTTCTACAAC GAAACTCTAT CGAAGTTTGC ATACAGCAAC
    TGCACTTCGG GAATTGTGAT GTACACGTTC AACGATAAAA AGTTTCCATC CACGTTCAGT
    TTCCTCACAG CGGGAGGCAT CATTGGTCTG TACACTACCT TTGTGTTATT GGCCTCGCGC
    TTTATGAAGT CCTTCATTGG CGGGCAAAAC AGAAAGATCA TGTTTGAGGA TCTGCCCTAT
    GTTGATAGAG TGCTGCAGCT CTGTCTGGAC ATTTATCTGG TACGGGAGGC ATTGGAATTC
    GCTTTGGAAG AGGACCTGTT TGCCAAATTA CTCTTCCTAT ACCGATCGCC CGAAACGCTA
    ATCAAGTGGA CGCGTCCCAA GGAGGAGTAC GTGGACGATG ATGGCGACAC CGACTCGATT
    CCCAGTCGAA TGAGCGTGCG CCGGCCGGAG CAACTGCAGC CACAGCAACC GCAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa41352
    Clone ID Related Accession (Same CDS sequence) NM_001316385.1
    Accession Version NM_001316385.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8922bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product piezo, isoform P
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    ATGGTCTTCA GCTATGCGTG CATGGTGCTC CAGCGCATCG TGGTGCCAGC GGTTCTGGTA 
    CTCGCTGCCC TGATGCGACC AGTGGGCATA TCCTTTGTCT ACCTTTTGAT GTTCTTTGTG
    TCGCCCTTTG TTCCGCTGGC CACGCGACGC AACTTCAAAG GATCTGTGAC TGCCTTCTTC
    ATCATCCTGC TGACGTTGAG CACGCTGGTC CTCTTGGGTC ACATAACGCT ACAGATTCTG
    GCGGTCAGCC TCACGCTGCC CATCTACAAT TGCTCGTTCA GTGAGCGTCT GCTGCGTCAC
    ATTGGCTTCG TGAGCTTTAT TGATCTACAG CCATTTGCCA TCATCGAATG GCTAGTGCCT
    GAGGTGTTGG TTTTCGCCAC CTCCCTGGGT TCGTATCTCA CGGTGAAGCG AGTGGCCTCA
    CAGCCCGTCG GCGCCGAGCA GCTGGAGAAC GGCGAGGTGG TCGATGGCCA GGCGGAAAAC
    GCGCAGACAT CTTCTCAGCC ATCTGCCGCA GATGCCAATG GAGGAGATGT GCAACAGGCC
    ACGGTCACCA CGCCGCTGCA GCAACAGCAG CAGCAGCTGA GGAAACGAGT GTCCATGATC
    AGCCAGCACA TACACTTTGA GGGCTTGGTC AAGATCTCTC CTCTGTTCTG CCTGGCCACG
    CTGTTCTTTG CGGCCGTACT GCGTCCCTCG GTGCCTGGCG GATTTTACTT TCTCATTTTC
    CTGCTGTCCG GCACCTACTG GGCAACATGC CAGACGCTGC AACGGGGCTT CGCTTTGTTG
    CTGCGCTGCG TGATGGTCGT CCTCGTGCTG CACTCCCTGT CCATTGTGTC ATACCAGACG
    CCATGGATGC AGAGCCACCT CAATCATACC ACCCTGACAG CTCGATTGAT TGGACTGGAA
    CCGCTTATTG AATCCTACTG CTCGCCGGAT ATACGAGTCT TTCTGTACAA TAATAAGCTA
    TCCCTGGACT CGTACCTCAA TCCCTTTGCG TTGTTCTTTG CCTATTTCGC ACTGGCACTG
    ACCACCAAGC ATCTGATTAA GCCACGGTTG GTGCGGCAAA GCACGCGAAA GGCACGTACA
    CCCCAACCGC TGGAAAGTGG ATCCTCGGTG GCGCCAAGTG TCACTCAGCG GGGCAACGAC
    ATGCAGCTGG AATCGATGGA GCAGCGATCG GAACAGGAGA ACACCACCAC ATCCATACTG
    GATCAAATAT CGTATGGATT CGTCAGCGTG GGAGGATTCA TATATCAGAA TAGCTATATA
    TTCACCAATA TTCTTATGAT GGCCTGGTCC ATAGTGTATC ACAGTTGGCT GACTTTTGTC
    CTCTTGCTGT CGGCCAACGT ACTGTGGATG ATTCCCAACC AGAGGAAGGC CATGATGCGG
    TCCAGTCCCT TTATAGTTCT GTATGCTGAG GCCCTTTTGA TTGCCCAATA CATATACGGC
    ATGGATCTCA ACAACGAAGA GCTGCCCACG AGCGTTCCCA CTGCGGGCAT TAACCTGCAA
    CAAATTGGCT TCGAACGACC CATCGAGAAC CAAATGCGGC CATGTGTGCC GCTGATCGTG
    AAGACAGCGT TTGTGCTAAT GTTTTGGGTG ACATCACGAC AGTTCTTCAA GGAGAAGCGC
    GATCGCCGAC GGGACAGCAC TCTGGCGGAC TTCATTGCCC CACTGCAGAT TACTGTGGGA
    TCGGCTGGCT CCAGCTACCT CATCAACGAT GGCAAGAAGA CCTCAAAGTT CCTAAAGAAG
    GCCGGCGATG TGATCAAAAA TCTATTGGTG CGTCTGTGGA TCTGGCTACT CGTGCTGGTT
    ATCTTCCTTT GCGCCATCAC TGGCGAGAAC ATGACCGGCT TCCGCATCTG CTACATGGCC
    CTGTTTCTAT TCTTCTTGCT AGTCTTTCAA TCTTCGTCCA AGGCGTGGGT TAAGATCATG
    TACGGCTTCT GGCTGTTTCT GATCTTCTAT GCCATGTCCA TACTTATATT GATCTACACA
    TATCAATTCG ACAAGTTCGA CACGTACTGG AGCGACTATC TTAATGTGTC CGCGACTTTG
    CAAAAGGACA TCGGCCTTAA GCGCTATCAG ACCAAGGATC TGTTCCTCCA TTTGGTCTCA
    CCGACGATAA TTGTGATCCT GACCGTCATA CAAGTGCACT ACTTCCACAA GCGCTTTATT
    GCATCATTGC AGCAGCAGCC GTTGGCTGGC GGATCGGCAC AGCAGAAACC CACGGAGACA
    ACTGCCTTGG AACCGGCGCC ATCGAAGCGA CGTGGCAGCG CCGGTTCACT GCGTAAATCT
    CAGGGTCCAT CGGCGGAGGC TGCTCCAGGA GCCACCACCG ATTTCGAGAC ATCTGTGCGA
    GACTTGGTGC GCATATCGTT CCGCAAGATC AAGAACAAGT CGGAGTACAT CTTCAAGAAC
    TTCAAGGATG TCTTCTGGCG CTTCCTGGAG CTGCACATCA TGAAGGCTGT GTATATCGCA
    GCCTTCGTGT GCAGTGTCAG CGAAGTCTGC GTACTGCACA TTATCTTTGT GGGTTTCTGT
    GTGCTGGGCG CCACCTCGCG GAAGGCCGTT CAGGTGGTGA TCAGCCGCCT CATCTCGTTC
    ATTGTCACCG TCATAGTTCT GTCCAAGATG ATCTACCAGA TCGAGTACTT GAGTCACTCG
    CAGCACAATG TGGTTTGTTC TGACAACCGG ACTGCCAACA ATGCGGAGTG GATTGGTCTC
    ACCAAGGCTG ACAAGGTAAC TGGCGGACTG ATGAGCCTGT TGCGCACCTA CATCATCTAC
    ATGGTTATTG TGACCATGCA CGCAGTGATC AGTTTGCGGC AGCTTCAAAT GCGCGTCAAG
    ATCGGAGCAC TGAATGCTCC ACCCACCAAG CTGCTGTTCC CCAATATTAT TCGAGCTGAT
    GCTGAGAAGG ATCTGGTGGG ACTGGTCAAG TATCTCCTCA ACTTTGGCTT CTACAAATTC
    GGCATTGAGA TATCGCTAAT CGCGCTGGTC TCTACCATCA CATATCGCCA GGATATTGTT
    GCCGTAGTCT ATGCTCTGTG GCTAGTGGTG CTTTTGCTTC TACGGAGATC GCAGTGCGCC
    AAAATATGGG GCGTGTTTCA GGCATTCTTT GCCATCTCCA TACTGACACA GTATATAGTG
    CTGGTAGGAT TGCCGCCGAG CTCATGCCTG GTGTTTCCCT GGGATGAAGG TCCCTTCGGC
    GAGGGCATAC AACGCTGGGC GATGCTGCCA GGAGCCCTGC ACTTCAACCA CGTACCCAAG
    CTGATCTTCG ACTTTATTGT CTTGGTCATT CTGAATCGAC AGAAGAGTAT CTTCTGCATC
    GAACAGCGTT ATGCCAGTAA CGATGACTAC CCGGGTGGCA GCAATCGCAG TGTGATCGCG
    GATATTGCTC AGCTAGGTCG CGTTCCCTTC GACAATCCCA CCCACGACTT TTGTTCGTAC
    ATACGCAACT ACTCGGACAT CCTTAAGAAC GGAGTGCTGT GCGGCTTCTA CTGGTTTACT
    CTGGCAGTTG TTTTCTTGGC CGGCACCAAT ATTGCTGATC TGCTGGCACT GGGTTATCTG
    ATCGGAGCGT TTATCTTCCT GTGGCAGGGA TCGGATTTCT ATCTGCGTCC CATACACACC
    ATCATCTTTC GCTGGAAGTG GCTGCTGGCA TTCAATGTGG CCAACATACT TATCAAGACG
    TCCTTCCAAA TGGCCGGCTG TTTGTTCATG ACCCAACTGA CGAAAGACTG CTGCTGGCTG
    GTGCACATGC TCGGCATCAC ATGTACGAGC AATGTACTTA CAGAGCAAAT AATGCTGCCC
    GAGGAGGCTG AATTGGCGCT TAAGCCAGGC GAATGTCCTA AGATCACCCA CCAGGTGGTC
    CTTCTGTGGG ACACGATTTG CTTTGCCTTC ATCATCTTCC AGCTGCGCAT CTTCAAGTCG
    CACTACTTCT GTCACATCAT AACGGACACC AAAGCAAATA ACATCCTGGC CTCAAGAGGA
    GCCGACATCA TTGAGAGCCT ACGACATAAG CAGATTGCAC ATCGACACGA CCATGAAAAG
    CAGGTGCTGC ATAAGATCAA GCGAAAGATG GAGCGCATCC GTGCCACGCA GCAGAAGATG
    CTACGACCCT TGGACAAACA AACCCACTTC GACGGGCGAC GCACGCCCTC AGAGCACGCC
    ATGTCCGATG TGGCGCCTTT GGTCCACAAT AGCAGCTTCA CCTCTTGTCA AGGATCTGGC
    TATCTGACCC CAGATGAGCA TAGCTCCGCC ACCTCAGGCA CTTCATCGCC AGCCAGGGCA
    TCGATCCTCT CCAGCAGTGG AGGCAGCGTG GAAAGCGTGG ACAGCGCAGA CGCTGCCGTG
    ATTATTGAAC CGGAAATCAA TGTGATGCCG TGTGATGAGA TTACGGACTA TGTGGATGTT
    CAGTCTGTCA GCGAGGAGGA GACAACTGTG GCAATGCCTA AAAAGGATCT TAGCTGCCAA
    TCAAAGGAAT GTGTTTTCAA AAGCCCAGAG CCTTCGAAGC CAACCACGCC GAGTACGGAT
    GCCTTGTCTA CTCTCCTGAC CGAGGGATTT TTGGAACCGA AAAGGATCTC GTTGGGATTG
    GCTCCTCCTG AGCTAAGTCC TCCTGTTGGG ATGCAGTGCC TGGCACCTCC GTTGGCTGCT
    TATGCCACAG CTATGGCTTC TAGTGCAGCC AGCTCTGTGC TGTCCACCAA TCTGACTCCA
    AATCTTCGCC GAGAACATCG GCGTCAGTCG TCAACCAATG CCGCTGTTTC CTGGAACGAA
    ACTGTATCCA TCAAACGATC GCCCATGAAA AAGCAAATGT CGGCGGATAA GGAGGAGGTG
    GTTACCATGC GGCACAAGTC GCTACACCGC CGACACCAGA GCATGGGAAA CGCCAGTTAC
    TCTTTGGACG AAGCCAGTCA GTCGCAGCGC AAGCGGTTGT CCAGCAACTT ATCCGGAGCT
    CGGGACGAGG CCGCTCTGCG ATCCGCACAG AGACCACATA GCTGGGGACC CGTGGGTACG
    AGCTCCTATA TGGAGTCGCT GATGTGCGCC TTCTACGAGC CTGCTCTGCT GCATGGACCA
    TCTACCCGTG CTGGTGACTA CTATATGTTC GAGGAGATGG ACGATAAGTT TGAGCTCGAC
    TTGATACACG ACGAGATCGA CTTCCTCGAG GAGGAGAACA TCACCGAGAG CGAGATGAAG
    ATGCAGCGAC GCAAGACCCT CTACGATGTG TGGAAGGATC TGAATGATGC CGAGTATCGT
    CGCCACTTCT ATATGCGTGA GCGTTCCTAC GCCTCAGAGC CCGGCTCACG CATTCTCCTG
    AAGTTGGACG ACGAGAAGGA GAAGATTGGG CCCGATCTCG GCCTGGCCAG CGACCACGAT
    GAGGACGACG ATACCATTGA GTGCGACCTT GGCATGCGCA CCTCGACGCT GTCCGAACCG
    CTCGACTTTG GTCGTCGCAG CCAGACGCTG CTCGAGGTTA AGTCGAAGGA TGCACCCACA
    GGCGAGTTTC CCTCCACCAG TAAGGGTATT TCGAAGGAGC GCGATGCGGC AACGGCTTCC
    AGTTCGGCTA GTCCAGCGCC CACTAGGGAT GTGGGTGATC TGCCTGTGAT TCCACCACCC
    TCCACTGGCC TGGGACGTGA GCAAACCTCC AAGGAGACCT CCGATAGCAA GTCCAAAATG
    GAAGTGGACA GCGGAGAGGT GACGGCCAAG GATTCGGATG AGGACTTCGA TACCAATCCC
    ATTATCAGAC TGCTCGAGGG CTTCTTGGTC ACGCTGACCA TAAGACTGAA CCGCTTTTCG
    CGCAACTATC GCTTTGTGAA TCGCATCCTG GCCGGCGAAA AGAAGACCCT AAAGGAGTCC
    AGTTCGTTGA ACCGTCTGGG GCTGTCCAGT GCCGCTGCCA TGTTCCACTT CCTCAAATCG
    AATTTAGAGA GCGATGAAAG TGAACCGCCC GCCTCCTCAT CCACCCCGCG GCGGGTGGTG
    ATCGCACCAC CGAATGCCAC CGAGCACTCA GACCCCACCA GCACCACACT GAACACGAAC
    ACGACAACCA CACCGCTATC ACCACCAGAA CCACTGCAAC CACTGCAACC ACTGCAACCA
    AATACAACCA GTACACCACA GCAACAGCAT CAGCATATTC GCGCTGCCGA AGAAATCATC
    GAACTGCCTG TAGATACCGT GGATGGAGTC GCCCATAGAA AACAATCAAT CAATTCATCG
    CCGCCAGCCA AGGGCACGAT GCTCAGTCGA AAATCGGATT GTGGCCTGCC TGAGATCCGT
    ATTAAAGCGC CATCTATCGA GCGCGGTGCA CACTATTACC ATAATCACCA CAGCGGCGGT
    GGCTCAGGAT CCTTGAGCAA ACACTGGTCC TACGAGCAGG TGGACAGCGC GGGTGAATTC
    AATCTGGAGG AGGAGAACTT CGCCCAGAGA GATCATCACA TCATTGTCGA GGTGTTGATC
    TCCTCGTGGT ATGCCCTGTT GGCCAACACG GATCTCATCT GCTACATTGT GGTTTTCATC
    AATCAGGTGG TCAATGCCAG TCTCATTTCG CTGCCGCTGC CCATTATGGT CTTTTTGTGG
    GGCACATTGT CTCTGCCGCG TCCCACTAAA ACCTTCTGGG TCACGCTGAT TGCCTACACC
    CAGGCCATCG TGCTGATCAA GTGCATCTTC CAGTTTAAGC TGATCTGGTC CAATTACCAC
    CAACTGCCCA ATCAGCCGCT AACACCGGCC AAAATATTCG GCGTTGAGAA TAAGGCCCAT
    TATGCGATTT ACGACCTGAT ACTATTGCTG GTTCTATTCC TGCATCGCTA TTTGCTTAAA
    TCACAAGGCC TGTGGAAATC GGGCTACAAG GACACGGATA ACCAGTTCAC CAAGCCCACC
    GCTAGCATTG ATGAACGCGA CGATAGCGAC AACTTATCCC AACCGGATTC CCGCCAGCTA
    AACGATGATG CTGCTCAGAA GCTGAGTCTC CAAGTGAGCC AGGCTTCCTT GCCAGGATCG
    CCCGAGTTTA GCAAGACAGG CATCAATCAG CTAGAGCGCA CCAAGTACAC TTCATCGCTG
    TACAAATTCT TCTTCAGTTT GGTCCACAAA TCCCGTCTAG CCACAGATGT TTACGCTCTG
    ATGTTCCTCT GCGATTTTGT GAACTTCTTC GTGCTTCTTT TCGGCTTCAC TGCTTTTGGA
    ACTCAGCAAA CGGAAAGTGA TGAAGGTGTG CAGACATATC TTGCGGAGAA CAAAGTGCCC
    ATACCATTCC TGATTATGCT ACTTGTTCAG TTCCTGCTCA TTGTCATTGA TCGAGCTTTG
    TATCTGCGCA AAGCCCTGGT GAACAAGATC ATCTTCCACT TCTTTTCGGT TATCGGAATA
    CACATCTGGA TGTTCTTCGT TGTGCCTGCA GTTACGGAGC GCACTTTCAA CTCCCTCGCA
    CCTCCAATAA TATTCTACGT TATCAAGTGC TTTTACATGC TGCTCAGCTC TTATCAAATC
    AAATCCGGTT ACCCTAAGCG CATTCTAGGC AACTTCTTCA CCAAGGGTTT CTCGATGGTC
    AATATGATTG CCTTTAAGGT GTACATGCAG ATTCCATTCC TGTACGAGCT GCGAACCATA
    TTAGATTGGG TGTGCATTGA CAGCACCATG ACCATTTTCG ATTGGCTAAA GATGGAGGAC
    ATTTTCTCGA ATATATATCT TATCCGCTGC ACCCGGCAGT CAGAGACTGA TTTTCCGGCC
    ATGAGAGCTC AGAAGAAGGC CTCACTTTCA AAGCTGATAA TGGGTGGCAC CATCGTCCTG
    CTGATAGTGA TTTGCATTTG GGGACCATTG TGTTTGTTTG CACTTGGCAA CGCGGTGGGC
    ACCTCGAATG TGCCGTTCCA TGTGTCGCTA TCAATACGAA TTGGACCCTA TGATCCCATC
    TACACAACGA ACAACTACGA CAGTATCTTC GAGATCAATC CTGAGATGTA CTCTCAGATG
    ACTAATGCAT ACATAAAGGA GAAACAGGCT TTGACGTTTA TTGCCGGCTA TGATGCAACG
    GATGTAGCGG CGGTTAGACT AGCTGGCAAC TCGCCATCTC TGTGGAACAT AGCACCGCCG
    GATAGGCAGC GACTACTAAA TGATTTAAGA AACAATCACA CGCTGAAGGC CCGGTTCTCT
    TATTCCCTCA CCCGAAAGGC TCCTGCCAAG GGGCTAAAGG AAAATGTGGG CGACGAGCAT
    GCCATATCGC TGGACGAGTC CTTTGAGGGA CGAGCAGCAC TCATTCACAT GCTAAGCGAA
    ACCCATGATG TGGAGCCCAT TTACAGCAAT GGCACCACAA ATGGAACCAC TCCCGAAGTT
    GAAGAAGTGG TGGTGATACC CGGAATGATA CCCAAGTTTA TCAAGGTTCT CAATTCGGGC
    GACGCTGCGG TGGTAAGTGT TTTGAGCCCA AAACACTACG ACTACCGACC GTTGGTTATC
    AAAATGCATC GGGACAACGA GACCAATGGG TTGTGGTGGG AGATTCGGGA CTATTGCAAC
    GACACCTTCT ACAACGAAAC TCTATCGAAG TTTGCATACA GCAACTGCAC TTCGGGAATT
    GTGATGTACA CGTTCAACGA TAAAAAGTTT CCATCCACGT TCAGTTTCCT CACAGCGGGA
    GGCATCATTG GTCTGTACAC TACCTTTGTG TTATTGGCCT CGCGCTTTAT GAAGTCCTTC
    ATTGGCGGGC AAAACAGAAA GATCATGTTT GAGGATCTGC CCTATGTTGA TAGAGTGCTG
    CAGCTCTGTC TGGACATTTA TCTGGTACGG GAGGCATTGG AATTCGCTTT GGAAGAGGAC
    CTGTTTGCCA AATTACTCTT CCTATACCGA TCGCCCGAAA CGCTAATCAA GTGGACGCGT
    CCCAAGGAGG AGTACGTGGA CGATGATGGC GACACCGACT CGATTCCCAG TCGAATGAGC
    GTGCGCCGGC CGGAGCAACT GCAGCCACAG CAACCGCAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001303314.1
    CDS577..9498
    Translation

    Target ORF information:

    RefSeq Version NM_001316385.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster piezo, transcript variant P (Piezo), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001316385.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    ATGGTCTTCA GCTATGCGTG CATGGTGCTC CAGCGCATCG TGGTGCCAGC GGTTCTGGTA 
    CTCGCTGCCC TGATGCGACC AGTGGGCATA TCCTTTGTCT ACCTTTTGAT GTTCTTTGTG
    TCGCCCTTTG TTCCGCTGGC CACGCGACGC AACTTCAAAG GATCTGTGAC TGCCTTCTTC
    ATCATCCTGC TGACGTTGAG CACGCTGGTC CTCTTGGGTC ACATAACGCT ACAGATTCTG
    GCGGTCAGCC TCACGCTGCC CATCTACAAT TGCTCGTTCA GTGAGCGTCT GCTGCGTCAC
    ATTGGCTTCG TGAGCTTTAT TGATCTACAG CCATTTGCCA TCATCGAATG GCTAGTGCCT
    GAGGTGTTGG TTTTCGCCAC CTCCCTGGGT TCGTATCTCA CGGTGAAGCG AGTGGCCTCA
    CAGCCCGTCG GCGCCGAGCA GCTGGAGAAC GGCGAGGTGG TCGATGGCCA GGCGGAAAAC
    GCGCAGACAT CTTCTCAGCC ATCTGCCGCA GATGCCAATG GAGGAGATGT GCAACAGGCC
    ACGGTCACCA CGCCGCTGCA GCAACAGCAG CAGCAGCTGA GGAAACGAGT GTCCATGATC
    AGCCAGCACA TACACTTTGA GGGCTTGGTC AAGATCTCTC CTCTGTTCTG CCTGGCCACG
    CTGTTCTTTG CGGCCGTACT GCGTCCCTCG GTGCCTGGCG GATTTTACTT TCTCATTTTC
    CTGCTGTCCG GCACCTACTG GGCAACATGC CAGACGCTGC AACGGGGCTT CGCTTTGTTG
    CTGCGCTGCG TGATGGTCGT CCTCGTGCTG CACTCCCTGT CCATTGTGTC ATACCAGACG
    CCATGGATGC AGAGCCACCT CAATCATACC ACCCTGACAG CTCGATTGAT TGGACTGGAA
    CCGCTTATTG AATCCTACTG CTCGCCGGAT ATACGAGTCT TTCTGTACAA TAATAAGCTA
    TCCCTGGACT CGTACCTCAA TCCCTTTGCG TTGTTCTTTG CCTATTTCGC ACTGGCACTG
    ACCACCAAGC ATCTGATTAA GCCACGGTTG GTGCGGCAAA GCACGCGAAA GGCACGTACA
    CCCCAACCGC TGGAAAGTGG ATCCTCGGTG GCGCCAAGTG TCACTCAGCG GGGCAACGAC
    ATGCAGCTGG AATCGATGGA GCAGCGATCG GAACAGGAGA ACACCACCAC ATCCATACTG
    GATCAAATAT CGTATGGATT CGTCAGCGTG GGAGGATTCA TATATCAGAA TAGCTATATA
    TTCACCAATA TTCTTATGAT GGCCTGGTCC ATAGTGTATC ACAGTTGGCT GACTTTTGTC
    CTCTTGCTGT CGGCCAACGT ACTGTGGATG ATTCCCAACC AGAGGAAGGC CATGATGCGG
    TCCAGTCCCT TTATAGTTCT GTATGCTGAG GCCCTTTTGA TTGCCCAATA CATATACGGC
    ATGGATCTCA ACAACGAAGA GCTGCCCACG AGCGTTCCCA CTGCGGGCAT TAACCTGCAA
    CAAATTGGCT TCGAACGACC CATCGAGAAC CAAATGCGGC CATGTGTGCC GCTGATCGTG
    AAGACAGCGT TTGTGCTAAT GTTTTGGGTG ACATCACGAC AGTTCTTCAA GGAGAAGCGC
    GATCGCCGAC GGGACAGCAC TCTGGCGGAC TTCATTGCCC CACTGCAGAT TACTGTGGGA
    TCGGCTGGCT CCAGCTACCT CATCAACGAT GGCAAGAAGA CCTCAAAGTT CCTAAAGAAG
    GCCGGCGATG TGATCAAAAA TCTATTGGTG CGTCTGTGGA TCTGGCTACT CGTGCTGGTT
    ATCTTCCTTT GCGCCATCAC TGGCGAGAAC ATGACCGGCT TCCGCATCTG CTACATGGCC
    CTGTTTCTAT TCTTCTTGCT AGTCTTTCAA TCTTCGTCCA AGGCGTGGGT TAAGATCATG
    TACGGCTTCT GGCTGTTTCT GATCTTCTAT GCCATGTCCA TACTTATATT GATCTACACA
    TATCAATTCG ACAAGTTCGA CACGTACTGG AGCGACTATC TTAATGTGTC CGCGACTTTG
    CAAAAGGACA TCGGCCTTAA GCGCTATCAG ACCAAGGATC TGTTCCTCCA TTTGGTCTCA
    CCGACGATAA TTGTGATCCT GACCGTCATA CAAGTGCACT ACTTCCACAA GCGCTTTATT
    GCATCATTGC AGCAGCAGCC GTTGGCTGGC GGATCGGCAC AGCAGAAACC CACGGAGACA
    ACTGCCTTGG AACCGGCGCC ATCGAAGCGA CGTGGCAGCG CCGGTTCACT GCGTAAATCT
    CAGGGTCCAT CGGCGGAGGC TGCTCCAGGA GCCACCACCG ATTTCGAGAC ATCTGTGCGA
    GACTTGGTGC GCATATCGTT CCGCAAGATC AAGAACAAGT CGGAGTACAT CTTCAAGAAC
    TTCAAGGATG TCTTCTGGCG CTTCCTGGAG CTGCACATCA TGAAGGCTGT GTATATCGCA
    GCCTTCGTGT GCAGTGTCAG CGAAGTCTGC GTACTGCACA TTATCTTTGT GGGTTTCTGT
    GTGCTGGGCG CCACCTCGCG GAAGGCCGTT CAGGTGGTGA TCAGCCGCCT CATCTCGTTC
    ATTGTCACCG TCATAGTTCT GTCCAAGATG ATCTACCAGA TCGAGTACTT GAGTCACTCG
    CAGCACAATG TGGTTTGTTC TGACAACCGG ACTGCCAACA ATGCGGAGTG GATTGGTCTC
    ACCAAGGCTG ACAAGGTAAC TGGCGGACTG ATGAGCCTGT TGCGCACCTA CATCATCTAC
    ATGGTTATTG TGACCATGCA CGCAGTGATC AGTTTGCGGC AGCTTCAAAT GCGCGTCAAG
    ATCGGAGCAC TGAATGCTCC ACCCACCAAG CTGCTGTTCC CCAATATTAT TCGAGCTGAT
    GCTGAGAAGG ATCTGGTGGG ACTGGTCAAG TATCTCCTCA ACTTTGGCTT CTACAAATTC
    GGCATTGAGA TATCGCTAAT CGCGCTGGTC TCTACCATCA CATATCGCCA GGATATTGTT
    GCCGTAGTCT ATGCTCTGTG GCTAGTGGTG CTTTTGCTTC TACGGAGATC GCAGTGCGCC
    AAAATATGGG GCGTGTTTCA GGCATTCTTT GCCATCTCCA TACTGACACA GTATATAGTG
    CTGGTAGGAT TGCCGCCGAG CTCATGCCTG GTGTTTCCCT GGGATGAAGG TCCCTTCGGC
    GAGGGCATAC AACGCTGGGC GATGCTGCCA GGAGCCCTGC ACTTCAACCA CGTACCCAAG
    CTGATCTTCG ACTTTATTGT CTTGGTCATT CTGAATCGAC AGAAGAGTAT CTTCTGCATC
    GAACAGCGTT ATGCCAGTAA CGATGACTAC CCGGGTGGCA GCAATCGCAG TGTGATCGCG
    GATATTGCTC AGCTAGGTCG CGTTCCCTTC GACAATCCCA CCCACGACTT TTGTTCGTAC
    ATACGCAACT ACTCGGACAT CCTTAAGAAC GGAGTGCTGT GCGGCTTCTA CTGGTTTACT
    CTGGCAGTTG TTTTCTTGGC CGGCACCAAT ATTGCTGATC TGCTGGCACT GGGTTATCTG
    ATCGGAGCGT TTATCTTCCT GTGGCAGGGA TCGGATTTCT ATCTGCGTCC CATACACACC
    ATCATCTTTC GCTGGAAGTG GCTGCTGGCA TTCAATGTGG CCAACATACT TATCAAGACG
    TCCTTCCAAA TGGCCGGCTG TTTGTTCATG ACCCAACTGA CGAAAGACTG CTGCTGGCTG
    GTGCACATGC TCGGCATCAC ATGTACGAGC AATGTACTTA CAGAGCAAAT AATGCTGCCC
    GAGGAGGCTG AATTGGCGCT TAAGCCAGGC GAATGTCCTA AGATCACCCA CCAGGTGGTC
    CTTCTGTGGG ACACGATTTG CTTTGCCTTC ATCATCTTCC AGCTGCGCAT CTTCAAGTCG
    CACTACTTCT GTCACATCAT AACGGACACC AAAGCAAATA ACATCCTGGC CTCAAGAGGA
    GCCGACATCA TTGAGAGCCT ACGACATAAG CAGATTGCAC ATCGACACGA CCATGAAAAG
    CAGGTGCTGC ATAAGATCAA GCGAAAGATG GAGCGCATCC GTGCCACGCA GCAGAAGATG
    CTACGACCCT TGGACAAACA AACCCACTTC GACGGGCGAC GCACGCCCTC AGAGCACGCC
    ATGTCCGATG TGGCGCCTTT GGTCCACAAT AGCAGCTTCA CCTCTTGTCA AGGATCTGGC
    TATCTGACCC CAGATGAGCA TAGCTCCGCC ACCTCAGGCA CTTCATCGCC AGCCAGGGCA
    TCGATCCTCT CCAGCAGTGG AGGCAGCGTG GAAAGCGTGG ACAGCGCAGA CGCTGCCGTG
    ATTATTGAAC CGGAAATCAA TGTGATGCCG TGTGATGAGA TTACGGACTA TGTGGATGTT
    CAGTCTGTCA GCGAGGAGGA GACAACTGTG GCAATGCCTA AAAAGGATCT TAGCTGCCAA
    TCAAAGGAAT GTGTTTTCAA AAGCCCAGAG CCTTCGAAGC CAACCACGCC GAGTACGGAT
    GCCTTGTCTA CTCTCCTGAC CGAGGGATTT TTGGAACCGA AAAGGATCTC GTTGGGATTG
    GCTCCTCCTG AGCTAAGTCC TCCTGTTGGG ATGCAGTGCC TGGCACCTCC GTTGGCTGCT
    TATGCCACAG CTATGGCTTC TAGTGCAGCC AGCTCTGTGC TGTCCACCAA TCTGACTCCA
    AATCTTCGCC GAGAACATCG GCGTCAGTCG TCAACCAATG CCGCTGTTTC CTGGAACGAA
    ACTGTATCCA TCAAACGATC GCCCATGAAA AAGCAAATGT CGGCGGATAA GGAGGAGGTG
    GTTACCATGC GGCACAAGTC GCTACACCGC CGACACCAGA GCATGGGAAA CGCCAGTTAC
    TCTTTGGACG AAGCCAGTCA GTCGCAGCGC AAGCGGTTGT CCAGCAACTT ATCCGGAGCT
    CGGGACGAGG CCGCTCTGCG ATCCGCACAG AGACCACATA GCTGGGGACC CGTGGGTACG
    AGCTCCTATA TGGAGTCGCT GATGTGCGCC TTCTACGAGC CTGCTCTGCT GCATGGACCA
    TCTACCCGTG CTGGTGACTA CTATATGTTC GAGGAGATGG ACGATAAGTT TGAGCTCGAC
    TTGATACACG ACGAGATCGA CTTCCTCGAG GAGGAGAACA TCACCGAGAG CGAGATGAAG
    ATGCAGCGAC GCAAGACCCT CTACGATGTG TGGAAGGATC TGAATGATGC CGAGTATCGT
    CGCCACTTCT ATATGCGTGA GCGTTCCTAC GCCTCAGAGC CCGGCTCACG CATTCTCCTG
    AAGTTGGACG ACGAGAAGGA GAAGATTGGG CCCGATCTCG GCCTGGCCAG CGACCACGAT
    GAGGACGACG ATACCATTGA GTGCGACCTT GGCATGCGCA CCTCGACGCT GTCCGAACCG
    CTCGACTTTG GTCGTCGCAG CCAGACGCTG CTCGAGGTTA AGTCGAAGGA TGCACCCACA
    GGCGAGTTTC CCTCCACCAG TAAGGGTATT TCGAAGGAGC GCGATGCGGC AACGGCTTCC
    AGTTCGGCTA GTCCAGCGCC CACTAGGGAT GTGGGTGATC TGCCTGTGAT TCCACCACCC
    TCCACTGGCC TGGGACGTGA GCAAACCTCC AAGGAGACCT CCGATAGCAA GTCCAAAATG
    GAAGTGGACA GCGGAGAGGT GACGGCCAAG GATTCGGATG AGGACTTCGA TACCAATCCC
    ATTATCAGAC TGCTCGAGGG CTTCTTGGTC ACGCTGACCA TAAGACTGAA CCGCTTTTCG
    CGCAACTATC GCTTTGTGAA TCGCATCCTG GCCGGCGAAA AGAAGACCCT AAAGGAGTCC
    AGTTCGTTGA ACCGTCTGGG GCTGTCCAGT GCCGCTGCCA TGTTCCACTT CCTCAAATCG
    AATTTAGAGA GCGATGAAAG TGAACCGCCC GCCTCCTCAT CCACCCCGCG GCGGGTGGTG
    ATCGCACCAC CGAATGCCAC CGAGCACTCA GACCCCACCA GCACCACACT GAACACGAAC
    ACGACAACCA CACCGCTATC ACCACCAGAA CCACTGCAAC CACTGCAACC ACTGCAACCA
    AATACAACCA GTACACCACA GCAACAGCAT CAGCATATTC GCGCTGCCGA AGAAATCATC
    GAACTGCCTG TAGATACCGT GGATGGAGTC GCCCATAGAA AACAATCAAT CAATTCATCG
    CCGCCAGCCA AGGGCACGAT GCTCAGTCGA AAATCGGATT GTGGCCTGCC TGAGATCCGT
    ATTAAAGCGC CATCTATCGA GCGCGGTGCA CACTATTACC ATAATCACCA CAGCGGCGGT
    GGCTCAGGAT CCTTGAGCAA ACACTGGTCC TACGAGCAGG TGGACAGCGC GGGTGAATTC
    AATCTGGAGG AGGAGAACTT CGCCCAGAGA GATCATCACA TCATTGTCGA GGTGTTGATC
    TCCTCGTGGT ATGCCCTGTT GGCCAACACG GATCTCATCT GCTACATTGT GGTTTTCATC
    AATCAGGTGG TCAATGCCAG TCTCATTTCG CTGCCGCTGC CCATTATGGT CTTTTTGTGG
    GGCACATTGT CTCTGCCGCG TCCCACTAAA ACCTTCTGGG TCACGCTGAT TGCCTACACC
    CAGGCCATCG TGCTGATCAA GTGCATCTTC CAGTTTAAGC TGATCTGGTC CAATTACCAC
    CAACTGCCCA ATCAGCCGCT AACACCGGCC AAAATATTCG GCGTTGAGAA TAAGGCCCAT
    TATGCGATTT ACGACCTGAT ACTATTGCTG GTTCTATTCC TGCATCGCTA TTTGCTTAAA
    TCACAAGGCC TGTGGAAATC GGGCTACAAG GACACGGATA ACCAGTTCAC CAAGCCCACC
    GCTAGCATTG ATGAACGCGA CGATAGCGAC AACTTATCCC AACCGGATTC CCGCCAGCTA
    AACGATGATG CTGCTCAGAA GCTGAGTCTC CAAGTGAGCC AGGCTTCCTT GCCAGGATCG
    CCCGAGTTTA GCAAGACAGG CATCAATCAG CTAGAGCGCA CCAAGTACAC TTCATCGCTG
    TACAAATTCT TCTTCAGTTT GGTCCACAAA TCCCGTCTAG CCACAGATGT TTACGCTCTG
    ATGTTCCTCT GCGATTTTGT GAACTTCTTC GTGCTTCTTT TCGGCTTCAC TGCTTTTGGA
    ACTCAGCAAA CGGAAAGTGA TGAAGGTGTG CAGACATATC TTGCGGAGAA CAAAGTGCCC
    ATACCATTCC TGATTATGCT ACTTGTTCAG TTCCTGCTCA TTGTCATTGA TCGAGCTTTG
    TATCTGCGCA AAGCCCTGGT GAACAAGATC ATCTTCCACT TCTTTTCGGT TATCGGAATA
    CACATCTGGA TGTTCTTCGT TGTGCCTGCA GTTACGGAGC GCACTTTCAA CTCCCTCGCA
    CCTCCAATAA TATTCTACGT TATCAAGTGC TTTTACATGC TGCTCAGCTC TTATCAAATC
    AAATCCGGTT ACCCTAAGCG CATTCTAGGC AACTTCTTCA CCAAGGGTTT CTCGATGGTC
    AATATGATTG CCTTTAAGGT GTACATGCAG ATTCCATTCC TGTACGAGCT GCGAACCATA
    TTAGATTGGG TGTGCATTGA CAGCACCATG ACCATTTTCG ATTGGCTAAA GATGGAGGAC
    ATTTTCTCGA ATATATATCT TATCCGCTGC ACCCGGCAGT CAGAGACTGA TTTTCCGGCC
    ATGAGAGCTC AGAAGAAGGC CTCACTTTCA AAGCTGATAA TGGGTGGCAC CATCGTCCTG
    CTGATAGTGA TTTGCATTTG GGGACCATTG TGTTTGTTTG CACTTGGCAA CGCGGTGGGC
    ACCTCGAATG TGCCGTTCCA TGTGTCGCTA TCAATACGAA TTGGACCCTA TGATCCCATC
    TACACAACGA ACAACTACGA CAGTATCTTC GAGATCAATC CTGAGATGTA CTCTCAGATG
    ACTAATGCAT ACATAAAGGA GAAACAGGCT TTGACGTTTA TTGCCGGCTA TGATGCAACG
    GATGTAGCGG CGGTTAGACT AGCTGGCAAC TCGCCATCTC TGTGGAACAT AGCACCGCCG
    GATAGGCAGC GACTACTAAA TGATTTAAGA AACAATCACA CGCTGAAGGC CCGGTTCTCT
    TATTCCCTCA CCCGAAAGGC TCCTGCCAAG GGGCTAAAGG AAAATGTGGG CGACGAGCAT
    GCCATATCGC TGGACGAGTC CTTTGAGGGA CGAGCAGCAC TCATTCACAT GCTAAGCGAA
    ACCCATGATG TGGAGCCCAT TTACAGCAAT GGCACCACAA ATGGAACCAC TCCCGAAGTT
    GAAGAAGTGG TGGTGATACC CGGAATGATA CCCAAGTTTA TCAAGGTTCT CAATTCGGGC
    GACGCTGCGG TGGTAAGTGT TTTGAGCCCA AAACACTACG ACTACCGACC GTTGGTTATC
    AAAATGCATC GGGACAACGA GACCAATGGG TTGTGGTGGG AGATTCGGGA CTATTGCAAC
    GACACCTTCT ACAACGAAAC TCTATCGAAG TTTGCATACA GCAACTGCAC TTCGGGAATT
    GTGATGTACA CGTTCAACGA TAAAAAGTTT CCATCCACGT TCAGTTTCCT CACAGCGGGA
    GGCATCATTG GTCTGTACAC TACCTTTGTG TTATTGGCCT CGCGCTTTAT GAAGTCCTTC
    ATTGGCGGGC AAAACAGAAA GATCATGTTT GAGGATCTGC CCTATGTTGA TAGAGTGCTG
    CAGCTCTGTC TGGACATTTA TCTGGTACGG GAGGCATTGG AATTCGCTTT GGAAGAGGAC
    CTGTTTGCCA AATTACTCTT CCTATACCGA TCGCCCGAAA CGCTAATCAA GTGGACGCGT
    CCCAAGGAGG AGTACGTGGA CGATGATGGC GACACCGACT CGATTCCCAG TCGAATGAGC
    GTGCGCCGGC CGGAGCAACT GCAGCCACAG CAACCGCAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.