Piezo2 cDNA ORF clone, Rattus norvegicus(Norway rat)

The following Piezo2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Piezo2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ORa54723 XM_017587882.1
Latest version!
Rattus norvegicus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
ORa54723 XM_017601121.1
Latest version!
Rattus norvegicus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
ORa20913 XM_006222595.3
Latest version!
Rattus norvegicus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ORa20913 XM_006254839.3
Latest version!
Rattus norvegicus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ORa20915 XM_225880.9
Latest version!
Rattus norvegicus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ORa20915 XM_001063568.6
Latest version!
Rattus norvegicus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ORa54716 XM_017587875.1
Latest version!
Rattus norvegicus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ORa54716 XM_017601114.1
Latest version!
Rattus norvegicus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ORa54717 XM_017587876.1
Latest version!
Rattus norvegicus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ORa54717 XM_017601115.1
Latest version!
Rattus norvegicus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ORa54718 XM_017587877.1
Latest version!
Rattus norvegicus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ORa54718 XM_017601116.1
Latest version!
Rattus norvegicus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ORa54719 XM_017587878.1
Latest version!
Rattus norvegicus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ORa54719 XM_017601117.1
Latest version!
Rattus norvegicus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ORa54720 XM_017587879.1
Latest version!
Rattus norvegicus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ORa54720 XM_017601118.1
Latest version!
Rattus norvegicus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ORa54721 XM_017601119.1
Latest version!
Rattus norvegicus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ORa54721 XM_017587880.1
Latest version!
Rattus norvegicus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ORa54722 XM_017601120.1
Latest version!
Rattus norvegicus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ORa54722 XM_017587881.1
Latest version!
Rattus norvegicus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ORa54723
    Clone ID Related Accession (Same CDS sequence) XM_017587882.1 , XM_017601121.1
    Accession Version XM_017587882.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7887bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product piezo-type mechanosensitive ion channel component 2 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000086.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTCCCCAT CTGCCTGGCA 
    GTAGCATGTG CATTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCG ACGATGCAAG GACACACAGG GCGGCTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCCTTT CTGCTCCTTC ACATCATTTT CCACATCACA
    CTGGCCAGCC TGGAAGCTCA GCACCGCATC GCACCCGCTT ACAACTGCTC AACTTGGGAA
    AAGACCTTCC GGCAGATCGG CTTCGAAAGC TTGAAGGGAG CTGATGCTGG CAATGGCATC
    AGGGTGTTTG TGCCTGATAT CGGGATGTTC ATTGCGAGCC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACGGAA GAAGTAGCCC AATTTAATTC CGAGTGCGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GACTCAGAAG AAGCGCTGAT TTATGAGGAG
    GACTTAGATG GGGAAGAAGG CATGGAAGGC GAGCTGGAAG AAAGCACAAA GTTAAAAATG
    TTTCGCAGGC TCGCCTCCGT CGCTTCCAAG CTTAAGGAGT TCATCGGCAA TATGATAACC
    ACGGCGGGCA AGGTGGTGGT GACCATCCTG TTGGGCTCCT CTGGCATGAT GCTGCCATCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT CTGGGTCTGT GCACCTGGTG GTCCTGGTGC
    CGAACTTTTG ACCCATTGCT GTTTGGCTGC CTCTGTGTCC TGTTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTTCAGTTCT TCCAAGAAGC AGTCCCACCC
    AATGACTACT ATGCAAGGTT GTTTGGAATC AAATCCGTTA TTCAAACAGA CTGCTCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTACAC GCTGGCCACG CTGATCCGCA TTTGGTTGCA AGAGCCTCTT
    GTGCAGGAGG AGATGTCGAA GGAAGAGGAG GGTGCACTGG ATGGCTGCTC CAATCAGAAC
    ACAGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    CTTTTATCCA TGACCCAAGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACTGTGAAC
    GGCAACCCTG TGGACTACCA CACCATCCAC CCAAGTCTGC CCATAGAGAA TGGCCCCGCC
    AAGACGGACT TGTATACTAC TCCTCAGTAC CGGTGGGAGC CCTCAGAGGA GTCCTCAGAA
    AAGAAAGAGG AGGAAGAGGA CAAGAGAGAA GACTCAGAAG GGGAAGGGAG CCAAGAGGAA
    AAACGGAGTG TGAGAATTCA TGCCATGGTC GCCGTGTTCC AGTTCATCAT GAAGCAGAGT
    TACATCTGTG CCCTCATCGC CATGATGGCA TGGAGCATCA CCTACCACAG CTGGCTGACA
    TTTGTGCTGT TGATCTGGTC GTGCACTCTC TGGATGATTC GCAACAGGAG GAAGTACGCC
    ATGATCAGCT CTCCGTTCAT GGTTGTCTAT GCCAATCTGC TGCTGGTACT GCAGTACATA
    TGGAGCTTTG AACTTCCAGA AATCAAGAAA GTCCCTGGAT TTCTAGAAAA GAAAGAGCCT
    GGAGAGCTTG CCTCAAAGAT CCTCTTCACC ATCACATTTT GGCTGCTCCT GAGGCAGCAT
    CTCACAGAGC AGAAGGCCCT TCAGGAAAAG GAAGCACTTC TGTCTGAAGT CAAAATTGGA
    AGTCAAGAAC TTGACGAAAA GGATGAGGAA CTCCAAGATA TACAAGTGGA AGGAGAGCCC
    ATAGAGAAGG AAGAAGAGGA GGAGGAAATA AAGGAAGACA GACATGAGGT AAAGAAGGAA
    GAGGAAGAGG AAGAGGAAGA TGACGATGAC CAGGACATCA TGAAGGTTCT GGGCAATCTG
    GTGGTGGCCC TCTTCATCAA GTATTGGATC TACGTCTGCG GGGGCATGTT CTTTTTTGTC
    AGCTTTGAGG GGAAAATCGT CATGTACAAA ATCATCTACA TGGTGCTGTT CCTGTTCTGT
    GTGGCCTTGT ACCAGGTGCA CTACGAGTGG TGGAGGAAGA TCCTGAAATA CTTCTGGATG
    TCGGTGGTCA TTTACACCAT GCTGGTGCTC ATCTTCATCT ACACTTACCA GTTTGAGAAC
    TTCCCAGGCC TGTGGCAAAA CATGACCGGG TTAAAGAAAG AGAAGCTTGA GGATCTGGGC
    CTGAAGCAGT TCACTGTGGC CGAACTGTTC ACACGAATAT TCATCCCCAC CTCCTTTCTG
    CTGGTCTGCA TCCTGCACCT CCACTATTTC CATGACCGGT TCCTGGAACT CACAGACCTC
    AAGTCCATCC CCAGCAAAGA AGACAACACC ATCTACAGCC ATGCCAAGGT CAATGGTCGC
    GTGTACCTAA TAATAAATAG CATCAAGAAA AAATTACCAA TCCACCAGAA TGAGTTAGCC
    CACCCTGAAG GAAGCCTCCC AGACCTAGCC ATTATGAACA TGACGGCCAG CCTCGATAAG
    CCTGAGAGGC AGAAGTTGGC AGAGCCTGGG GAGGAGAGGA CAGAAGAATG TGCCAAGAAG
    GCAGAGAAGG GTGAGGCTGG GAAAGACAGT GATGAGTCTG AGGAGGAGGA AGGTGAGGAA
    GAGGAATCCG AGGAGGAAGA ATCCTCAGAT CTCAGGAACA AGTGGCACCT CGTGATTGAC
    CGGCTCACTG TGCTCTTTCT AAAGTTCCTG GAGTATTTCC ACAAGTTGCA GGTGTTTATG
    TGGTGGATCT TGGAGCTGCA CATCATCAAA ATCGTCTCAT CGTACATTAT CTGGGTGACT
    GTCAAAGAGG TGTCTCTGTT CAACTATGTA TTTTTGATTT CTTGGGCATT TGCTCTGCCA
    TATGCCAAAC TCCGCCGTGT GGCTTCCAGT GTCTGCACTG TCTGGACGTG TGTGATCATC
    GTCTGCAAAA TGTTGTACCA GCTGCAAACC ATTAAGCCTG AGAACTTTTC TGTCAACTGC
    TCCTTGCCCA ATGAAAATCA AACCAACATA CCCCTTAATG AGTTGAACAA GTCTCTTCTC
    TACAGTGCCC CTGTTGATCC CACTGAGTGG GTTGGCCTGA GGAAGTCCTC CCCTTTACTC
    GTCTACCTGA GGAACAACCT CCTGATGTTG GCGATTTTGG CCTTCGAAGT CACTGTTTAC
    CGACATCAGG AGTACTATCG AGGCCGAAAC AACTTGACTG CTCCGGTGTC TAAAACTATC
    TTTCATGACA TCACAAGGTT ACATCTAGAT GATGGGCTTA TCAATTGTGC CAAATATTTT
    GTAAACTACT TCTTCTACAA GTTTGGCCTA GAGACCTGTT TCCTAATGTC AGTGAACGTA
    ATTGGACAGC GAATGGACTT CTACGCCATG ATTCATGCCT GTTGGCTGAT CGGTGTCTTG
    TACCGTCGCA GAAGAAAAGC CATTGCTGAG GTCTGGCCCA AGTACTGCTG CTTCCTGGCT
    TGCATCATCA CCTTCCAGTA CTTTGTCTGC ATAGGCATCC CTCCTGCCCC CTGCCGAGAT
    TACCCATGGA GGTTCAAGGG TGCCAACTTC AATGACAACA TCATCAAGTG GCTGTACTTC
    CCAGATTTCA TCGTGCGGCC CAACCCTGTG TTTCTTGTCT ATGACTTCAT GCTGCTCCTG
    TGTGCTTCCT TACAACGACA GATTTTTGAG GACGAAAACA AGGCAGCTGT GCGCATCATG
    GCCGGGGACA ATGTCGAGAT TTGCATGAAC CTTGACGCGG CCTCATTCAG CCAGCATAAC
    CCTGTACCAG ATTTCATTCA CTGCAGATCT TACTTGGACA TGTCCAAAGT GATCATCTTC
    AGCTACCTCT TCTGGTTCGT CCTCACCATC ATCTTCATTA CCGGAACGAC CAGGATCAGC
    ATCTTTTGCA TGGGTTACCT GGTGGCCTGT TTCTATTTCC TGCTCTTTGG AGGTGATCTG
    CTCCTGAAAC CCATCAAGAG CATCCTACGC TACTGGGACT GGCTGATTGC ATACAACGTG
    TTTGTGATCA CCATGAAAAA CATACTGTCA ATAGGAGCCT GTGGATACAT CGGTGCACTG
    GTGAGAAACA GCTGCTGGCT GATCCAAGCG TTCAGCCTGG CCTGCACGGT GAAAGGCTAT
    AAAATGCCTG AAGATGACTC CAGATGCAAG CTGCCCAGTG GGGAGGCAGG GATCATCTGG
    GACAGCATCT GCTTTGCGTT TCTCCTGCTA CAGAGGAGAG TCTTCATGAG TTACTATTTC
    CTGCATGTCG TGGCTGACAT TAAAGCATCC CAGATCCTGG CATCCAGAGG GGCCGAACTT
    TTCCAGGCTA CAATTGTGAA AGCTGTAAAG GCAAGAATTG AAGAAGAGAA AAAATCCATG
    GACCAGCTGA AGAGACAGAT GGATCGCATC AAAGCCCGGC AGCAAAAGTA CAAAAAGGGT
    AAGGAGAGGA TGCTGAGCTT GACCCAGGAG TCAGGGGAAG GCCAGGACAT CCAGAAAGTC
    TCAGAAGAAG ACGATGAAAG AGAAGCAGAC AAACAGAAAG CCAAGGGCAA AAAAAAGCAG
    TGGTGGCGGC CTTGGGTTGA TCATGCTTCC ATGGTCAGGA GTGGAGATTA TTATTTGTTT
    GAAACGGATA GTGAAGAGGA GGAAGAAGAG GAACTCAAAA AGGAGGATGA AGAACCACCA
    CGGAAGTCTG CATTCCAGAG AGCTATTGGG AAGTTTGCCT CAGCCATTTT AGCCTTGCCA
    AAGTCTGTCA TTAAACTTCC CAAAACTATT CTTCAGTATT TAATCAGAGC TGCAAAGTTC
    GTCTACCAAG CCTGGATTAC CGATCCTAAA ACAGCACTCC GACAGAGGAG GAAAGAGAAG
    AAGAAAATGG CCAGAGAAGA GCAGAAGGGA AGACGCAAAG GATCTGGGGA GGGTCCAGTG
    GAATGGGAAG ACAGAGAAGA TGAGCCAATC AAAAAGAAAT CTGATGGACC AGATAATATC
    ATCAAGAGGA TATTTAATAT CCTGAAGTTC ACCTGGGTCC TGTTCCTGGC CACAGTGGAC
    AGTTTCACTA CTTGGCTCAA CTCCATCTCA AGAGAGCATA TCGACATATC CACAGTTCTG
    CGGATTGAAA GATGCATGCT GACCCGAGAG ATAAAGAAGG GCAACGTTCC GACACGGGAA
    AGCATCCACA TGTACTACCA GAACCACATG ATGAACCTTT CCAGAGAATC CGGACTGGAC
    ACAATCGACG AGCACTCTGG CGCTGGTTCC AGGGCCCAGG CAGCACACAG GATGGATAGC
    TTAGATTCCC ATGACAGTAT CTCCAGCTGT TACACTGAAG CAACCCTGCT GATCTCAAGG
    CAGTCCACCC TTGATGATTT AGATGGACAA GACCCTGTTC CTAAAACAAG CGAGCGTGCT
    CGGCCTAGGC TCCGTAAAGT GTTCAGCATG GACATGTCCT CCTCATCAGC TGACAGTGGC
    AGTGTAGCAT CAAGCGAGCC CACACAGTGC ACCATGCTGT ATTCACGCCA AGGAACTACG
    GAAACCATTG AGGAGGTAGA GGCTGAAGCT GAGGAGGAGG TGGTAGAGAG TCTGGAGCCT
    GAGCTCCATG ATGCCGAAGA GAAGGAATAT GCAGCTGACT ATGAAGCAGG GGTAGAGGAG
    GTCAGCCTCA CTCCAGATGA GGAGTTGCCT CAGTTCTCAA CAGATGACGG TGACGTGGAG
    GCCCCGCCCT CCTACAGCAA GGCTGTCAGC TTTGAGCACC TCTCGTTTGC ATCACAAGAT
    GACTCCGGGG GCAAGAACCA CATGGTGGTC AGCCCTGACG ACAGCCGCAC AGACAAGCTG
    GAGTCCAGTA TCTTACCGCC CCTGACTCAC GAGCTGACAG CCAGCGATCT GCTCATGAGC
    AAGATGTTCC GTGATGATGA GCTTGAAGAA TCTGAGAAAT TCTACGTGGA CCAGCCCCGG
    TTCTTGCTGC TATTCTATGC CATGTATAAC ACTCTGGTAG CCCGCTCGGA AATGGTGTGC
    TATTTCGTGA TCATCCTGAA CCACATGATC TCTGCCTCCA TCATTACTCT TCTGTTGCCC
    ATCCTGATCT TCCTCTGGGC CATGCTGTCC GTCCCCAGGC CCAGCCGGCG CTTCTGGATG
    ATGGCCATCG TCTACACTGA GGTGGCGATT GTAGTGAAAT ATTTCTTCCA GTTTGGCTTC
    TTTCCTTGGA ATAAGGACTT GGAAATTTAC AAGGACAGGC CCTACTTTCC ACCAAACATC
    ATAGGGGTGG AAAAGAAAGA GGGCTATGTT CTTTACGACC TCATTCAGCT GCTGGCTCTG
    TTCTTCCATC GATCCATTCT GAAGTGTCAT GGCCTATGGG ACGAGGATGA CATTGTTGAC
    AGTAACACAG ACAAGGAAGG ATCTGATGAT GAGCTGTCCC TCGGTCAGGG CAGAAGAGGC
    TCCTCAGAGT CACTGAAGTC CATCAACTTG GCTGCCTCTG TAGAGTCCGT CCATGTGACC
    TTCCCAGAGC AGCCAGCAGC CATCCGGAGA AAACGCTCCT GCAGCAGCTC GCAGATCTCC
    CCACGGTCCA GCTTCTCTTC AAATAGGTCC AAAAGAGGCA GCACAAGCAC CCGAAACAGC
    AGCCAAAAAG GAAGCAGTGT TTTGAGCATT AAACAAAAAA GCAAAAGAGA ACTTTACATG
    GAAAAGCTTC AAGAACATTT AATCAAAGCA AAGGCCTTTA CCATAAAGAA AACTCTACAA
    ATCTATGTAC CCATTCGGCA ATTCTTCTAT GACCTCATCC ACCCAGACTA CAGTGCTGTG
    ACTGACGTGT ATGTGCTGAT GTTCCTGGCC GACACCGTGG ACTTCATCAT CATCGTCTTT
    GGCTTCTGGG CTTTTGGGAA ACACTCGGCA GCTGCAGATA TCACCTCTTC CCTATCAGAA
    GACCAGGTCC CCGGGCCATT CCTGGTGATG GTTCTCATTC AGTTTGGAAC CATGGTGGTG
    GACCGTGCAC TCTACCTCAG GAAGACTGTG CTTGGCAAGG TCATTTTCCA GGTCATCCTC
    GTGTTTGGAA TTCACTTCTG GATGTTCTTC ATCCTACCCA GTGTGACTGA GAGGAAATTC
    AGCCAGAACC TGGTGGCACA GCTCTGGTAC TTTGTGAAAT GTGTGTACTT TGGGTTGTCT
    GCCTACCAAA TCCGCTGTGG CTACCCAACA CGTGTGCTCG GAAACTTCCT CACGAAGAGC
    TACAATTATG TCAACCTCTT CTTGTTTCAA GGGTTTCGTC TCGTCCCATT TCTGACAGAG
    CTGCGGGCTG TGATGGACTG GGTGTGGACA GATACAACCT TGAGCCTCTC CAGCTGGATT
    TGTGTGGAGG ACATTTACGC ACACATTTTC ATCTTAAAGT GCTGGCGAGA GTCGGAAAAG
    AGATACCCTC AACCCAGGGG CCAGAAGAAG AAGAAAGCTG TGAAGTATGG CATGGGCGGA
    ATGATCATTG TCCTGCTCAT CTGTATCGTC TGGTTTCCTC TGCTCTTCAT GTCTTTGATC
    AAGTCTGTCG CAGGGGTTAT TAACCAACCC CTGGATGTGT CCGTCACAAT AACCCTGGGA
    GGCTATCAGC CTATCTTCAC AATGAGTGCC CAGCAAAGCC AGCTGAAAGT TATGGATGGT
    ACAAAGTATA ACGAATTCAT AAAGTCTTTC GGCCCCAACA GTGGCGCTAT GCAATTTCTG
    GAGAATTATG AAAGGCAAGA CATAACAGTA GCGGAACTGG AAGGGAATTC GAATTCTTTG
    TGGACCATCA GCCCTCCCAA AACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017443371.1
    CDS943..8829
    Translation

    Target ORF information:

    RefSeq Version XM_017587882.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017587882.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTCCCCAT CTGCCTGGCA 
    GTAGCATGTG CATTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCG ACGATGCAAG GACACACAGG GCGGCTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCCTTT CTGCTCCTTC ACATCATTTT CCACATCACA
    CTGGCCAGCC TGGAAGCTCA GCACCGCATC GCACCCGCTT ACAACTGCTC AACTTGGGAA
    AAGACCTTCC GGCAGATCGG CTTCGAAAGC TTGAAGGGAG CTGATGCTGG CAATGGCATC
    AGGGTGTTTG TGCCTGATAT CGGGATGTTC ATTGCGAGCC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACGGAA GAAGTAGCCC AATTTAATTC CGAGTGCGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GACTCAGAAG AAGCGCTGAT TTATGAGGAG
    GACTTAGATG GGGAAGAAGG CATGGAAGGC GAGCTGGAAG AAAGCACAAA GTTAAAAATG
    TTTCGCAGGC TCGCCTCCGT CGCTTCCAAG CTTAAGGAGT TCATCGGCAA TATGATAACC
    ACGGCGGGCA AGGTGGTGGT GACCATCCTG TTGGGCTCCT CTGGCATGAT GCTGCCATCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT CTGGGTCTGT GCACCTGGTG GTCCTGGTGC
    CGAACTTTTG ACCCATTGCT GTTTGGCTGC CTCTGTGTCC TGTTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTTCAGTTCT TCCAAGAAGC AGTCCCACCC
    AATGACTACT ATGCAAGGTT GTTTGGAATC AAATCCGTTA TTCAAACAGA CTGCTCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTACAC GCTGGCCACG CTGATCCGCA TTTGGTTGCA AGAGCCTCTT
    GTGCAGGAGG AGATGTCGAA GGAAGAGGAG GGTGCACTGG ATGGCTGCTC CAATCAGAAC
    ACAGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    CTTTTATCCA TGACCCAAGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACTGTGAAC
    GGCAACCCTG TGGACTACCA CACCATCCAC CCAAGTCTGC CCATAGAGAA TGGCCCCGCC
    AAGACGGACT TGTATACTAC TCCTCAGTAC CGGTGGGAGC CCTCAGAGGA GTCCTCAGAA
    AAGAAAGAGG AGGAAGAGGA CAAGAGAGAA GACTCAGAAG GGGAAGGGAG CCAAGAGGAA
    AAACGGAGTG TGAGAATTCA TGCCATGGTC GCCGTGTTCC AGTTCATCAT GAAGCAGAGT
    TACATCTGTG CCCTCATCGC CATGATGGCA TGGAGCATCA CCTACCACAG CTGGCTGACA
    TTTGTGCTGT TGATCTGGTC GTGCACTCTC TGGATGATTC GCAACAGGAG GAAGTACGCC
    ATGATCAGCT CTCCGTTCAT GGTTGTCTAT GCCAATCTGC TGCTGGTACT GCAGTACATA
    TGGAGCTTTG AACTTCCAGA AATCAAGAAA GTCCCTGGAT TTCTAGAAAA GAAAGAGCCT
    GGAGAGCTTG CCTCAAAGAT CCTCTTCACC ATCACATTTT GGCTGCTCCT GAGGCAGCAT
    CTCACAGAGC AGAAGGCCCT TCAGGAAAAG GAAGCACTTC TGTCTGAAGT CAAAATTGGA
    AGTCAAGAAC TTGACGAAAA GGATGAGGAA CTCCAAGATA TACAAGTGGA AGGAGAGCCC
    ATAGAGAAGG AAGAAGAGGA GGAGGAAATA AAGGAAGACA GACATGAGGT AAAGAAGGAA
    GAGGAAGAGG AAGAGGAAGA TGACGATGAC CAGGACATCA TGAAGGTTCT GGGCAATCTG
    GTGGTGGCCC TCTTCATCAA GTATTGGATC TACGTCTGCG GGGGCATGTT CTTTTTTGTC
    AGCTTTGAGG GGAAAATCGT CATGTACAAA ATCATCTACA TGGTGCTGTT CCTGTTCTGT
    GTGGCCTTGT ACCAGGTGCA CTACGAGTGG TGGAGGAAGA TCCTGAAATA CTTCTGGATG
    TCGGTGGTCA TTTACACCAT GCTGGTGCTC ATCTTCATCT ACACTTACCA GTTTGAGAAC
    TTCCCAGGCC TGTGGCAAAA CATGACCGGG TTAAAGAAAG AGAAGCTTGA GGATCTGGGC
    CTGAAGCAGT TCACTGTGGC CGAACTGTTC ACACGAATAT TCATCCCCAC CTCCTTTCTG
    CTGGTCTGCA TCCTGCACCT CCACTATTTC CATGACCGGT TCCTGGAACT CACAGACCTC
    AAGTCCATCC CCAGCAAAGA AGACAACACC ATCTACAGCC ATGCCAAGGT CAATGGTCGC
    GTGTACCTAA TAATAAATAG CATCAAGAAA AAATTACCAA TCCACCAGAA TGAGTTAGCC
    CACCCTGAAG GAAGCCTCCC AGACCTAGCC ATTATGAACA TGACGGCCAG CCTCGATAAG
    CCTGAGAGGC AGAAGTTGGC AGAGCCTGGG GAGGAGAGGA CAGAAGAATG TGCCAAGAAG
    GCAGAGAAGG GTGAGGCTGG GAAAGACAGT GATGAGTCTG AGGAGGAGGA AGGTGAGGAA
    GAGGAATCCG AGGAGGAAGA ATCCTCAGAT CTCAGGAACA AGTGGCACCT CGTGATTGAC
    CGGCTCACTG TGCTCTTTCT AAAGTTCCTG GAGTATTTCC ACAAGTTGCA GGTGTTTATG
    TGGTGGATCT TGGAGCTGCA CATCATCAAA ATCGTCTCAT CGTACATTAT CTGGGTGACT
    GTCAAAGAGG TGTCTCTGTT CAACTATGTA TTTTTGATTT CTTGGGCATT TGCTCTGCCA
    TATGCCAAAC TCCGCCGTGT GGCTTCCAGT GTCTGCACTG TCTGGACGTG TGTGATCATC
    GTCTGCAAAA TGTTGTACCA GCTGCAAACC ATTAAGCCTG AGAACTTTTC TGTCAACTGC
    TCCTTGCCCA ATGAAAATCA AACCAACATA CCCCTTAATG AGTTGAACAA GTCTCTTCTC
    TACAGTGCCC CTGTTGATCC CACTGAGTGG GTTGGCCTGA GGAAGTCCTC CCCTTTACTC
    GTCTACCTGA GGAACAACCT CCTGATGTTG GCGATTTTGG CCTTCGAAGT CACTGTTTAC
    CGACATCAGG AGTACTATCG AGGCCGAAAC AACTTGACTG CTCCGGTGTC TAAAACTATC
    TTTCATGACA TCACAAGGTT ACATCTAGAT GATGGGCTTA TCAATTGTGC CAAATATTTT
    GTAAACTACT TCTTCTACAA GTTTGGCCTA GAGACCTGTT TCCTAATGTC AGTGAACGTA
    ATTGGACAGC GAATGGACTT CTACGCCATG ATTCATGCCT GTTGGCTGAT CGGTGTCTTG
    TACCGTCGCA GAAGAAAAGC CATTGCTGAG GTCTGGCCCA AGTACTGCTG CTTCCTGGCT
    TGCATCATCA CCTTCCAGTA CTTTGTCTGC ATAGGCATCC CTCCTGCCCC CTGCCGAGAT
    TACCCATGGA GGTTCAAGGG TGCCAACTTC AATGACAACA TCATCAAGTG GCTGTACTTC
    CCAGATTTCA TCGTGCGGCC CAACCCTGTG TTTCTTGTCT ATGACTTCAT GCTGCTCCTG
    TGTGCTTCCT TACAACGACA GATTTTTGAG GACGAAAACA AGGCAGCTGT GCGCATCATG
    GCCGGGGACA ATGTCGAGAT TTGCATGAAC CTTGACGCGG CCTCATTCAG CCAGCATAAC
    CCTGTACCAG ATTTCATTCA CTGCAGATCT TACTTGGACA TGTCCAAAGT GATCATCTTC
    AGCTACCTCT TCTGGTTCGT CCTCACCATC ATCTTCATTA CCGGAACGAC CAGGATCAGC
    ATCTTTTGCA TGGGTTACCT GGTGGCCTGT TTCTATTTCC TGCTCTTTGG AGGTGATCTG
    CTCCTGAAAC CCATCAAGAG CATCCTACGC TACTGGGACT GGCTGATTGC ATACAACGTG
    TTTGTGATCA CCATGAAAAA CATACTGTCA ATAGGAGCCT GTGGATACAT CGGTGCACTG
    GTGAGAAACA GCTGCTGGCT GATCCAAGCG TTCAGCCTGG CCTGCACGGT GAAAGGCTAT
    AAAATGCCTG AAGATGACTC CAGATGCAAG CTGCCCAGTG GGGAGGCAGG GATCATCTGG
    GACAGCATCT GCTTTGCGTT TCTCCTGCTA CAGAGGAGAG TCTTCATGAG TTACTATTTC
    CTGCATGTCG TGGCTGACAT TAAAGCATCC CAGATCCTGG CATCCAGAGG GGCCGAACTT
    TTCCAGGCTA CAATTGTGAA AGCTGTAAAG GCAAGAATTG AAGAAGAGAA AAAATCCATG
    GACCAGCTGA AGAGACAGAT GGATCGCATC AAAGCCCGGC AGCAAAAGTA CAAAAAGGGT
    AAGGAGAGGA TGCTGAGCTT GACCCAGGAG TCAGGGGAAG GCCAGGACAT CCAGAAAGTC
    TCAGAAGAAG ACGATGAAAG AGAAGCAGAC AAACAGAAAG CCAAGGGCAA AAAAAAGCAG
    TGGTGGCGGC CTTGGGTTGA TCATGCTTCC ATGGTCAGGA GTGGAGATTA TTATTTGTTT
    GAAACGGATA GTGAAGAGGA GGAAGAAGAG GAACTCAAAA AGGAGGATGA AGAACCACCA
    CGGAAGTCTG CATTCCAGAG AGCTATTGGG AAGTTTGCCT CAGCCATTTT AGCCTTGCCA
    AAGTCTGTCA TTAAACTTCC CAAAACTATT CTTCAGTATT TAATCAGAGC TGCAAAGTTC
    GTCTACCAAG CCTGGATTAC CGATCCTAAA ACAGCACTCC GACAGAGGAG GAAAGAGAAG
    AAGAAAATGG CCAGAGAAGA GCAGAAGGGA AGACGCAAAG GATCTGGGGA GGGTCCAGTG
    GAATGGGAAG ACAGAGAAGA TGAGCCAATC AAAAAGAAAT CTGATGGACC AGATAATATC
    ATCAAGAGGA TATTTAATAT CCTGAAGTTC ACCTGGGTCC TGTTCCTGGC CACAGTGGAC
    AGTTTCACTA CTTGGCTCAA CTCCATCTCA AGAGAGCATA TCGACATATC CACAGTTCTG
    CGGATTGAAA GATGCATGCT GACCCGAGAG ATAAAGAAGG GCAACGTTCC GACACGGGAA
    AGCATCCACA TGTACTACCA GAACCACATG ATGAACCTTT CCAGAGAATC CGGACTGGAC
    ACAATCGACG AGCACTCTGG CGCTGGTTCC AGGGCCCAGG CAGCACACAG GATGGATAGC
    TTAGATTCCC ATGACAGTAT CTCCAGCTGT TACACTGAAG CAACCCTGCT GATCTCAAGG
    CAGTCCACCC TTGATGATTT AGATGGACAA GACCCTGTTC CTAAAACAAG CGAGCGTGCT
    CGGCCTAGGC TCCGTAAAGT GTTCAGCATG GACATGTCCT CCTCATCAGC TGACAGTGGC
    AGTGTAGCAT CAAGCGAGCC CACACAGTGC ACCATGCTGT ATTCACGCCA AGGAACTACG
    GAAACCATTG AGGAGGTAGA GGCTGAAGCT GAGGAGGAGG TGGTAGAGAG TCTGGAGCCT
    GAGCTCCATG ATGCCGAAGA GAAGGAATAT GCAGCTGACT ATGAAGCAGG GGTAGAGGAG
    GTCAGCCTCA CTCCAGATGA GGAGTTGCCT CAGTTCTCAA CAGATGACGG TGACGTGGAG
    GCCCCGCCCT CCTACAGCAA GGCTGTCAGC TTTGAGCACC TCTCGTTTGC ATCACAAGAT
    GACTCCGGGG GCAAGAACCA CATGGTGGTC AGCCCTGACG ACAGCCGCAC AGACAAGCTG
    GAGTCCAGTA TCTTACCGCC CCTGACTCAC GAGCTGACAG CCAGCGATCT GCTCATGAGC
    AAGATGTTCC GTGATGATGA GCTTGAAGAA TCTGAGAAAT TCTACGTGGA CCAGCCCCGG
    TTCTTGCTGC TATTCTATGC CATGTATAAC ACTCTGGTAG CCCGCTCGGA AATGGTGTGC
    TATTTCGTGA TCATCCTGAA CCACATGATC TCTGCCTCCA TCATTACTCT TCTGTTGCCC
    ATCCTGATCT TCCTCTGGGC CATGCTGTCC GTCCCCAGGC CCAGCCGGCG CTTCTGGATG
    ATGGCCATCG TCTACACTGA GGTGGCGATT GTAGTGAAAT ATTTCTTCCA GTTTGGCTTC
    TTTCCTTGGA ATAAGGACTT GGAAATTTAC AAGGACAGGC CCTACTTTCC ACCAAACATC
    ATAGGGGTGG AAAAGAAAGA GGGCTATGTT CTTTACGACC TCATTCAGCT GCTGGCTCTG
    TTCTTCCATC GATCCATTCT GAAGTGTCAT GGCCTATGGG ACGAGGATGA CATTGTTGAC
    AGTAACACAG ACAAGGAAGG ATCTGATGAT GAGCTGTCCC TCGGTCAGGG CAGAAGAGGC
    TCCTCAGAGT CACTGAAGTC CATCAACTTG GCTGCCTCTG TAGAGTCCGT CCATGTGACC
    TTCCCAGAGC AGCCAGCAGC CATCCGGAGA AAACGCTCCT GCAGCAGCTC GCAGATCTCC
    CCACGGTCCA GCTTCTCTTC AAATAGGTCC AAAAGAGGCA GCACAAGCAC CCGAAACAGC
    AGCCAAAAAG GAAGCAGTGT TTTGAGCATT AAACAAAAAA GCAAAAGAGA ACTTTACATG
    GAAAAGCTTC AAGAACATTT AATCAAAGCA AAGGCCTTTA CCATAAAGAA AACTCTACAA
    ATCTATGTAC CCATTCGGCA ATTCTTCTAT GACCTCATCC ACCCAGACTA CAGTGCTGTG
    ACTGACGTGT ATGTGCTGAT GTTCCTGGCC GACACCGTGG ACTTCATCAT CATCGTCTTT
    GGCTTCTGGG CTTTTGGGAA ACACTCGGCA GCTGCAGATA TCACCTCTTC CCTATCAGAA
    GACCAGGTCC CCGGGCCATT CCTGGTGATG GTTCTCATTC AGTTTGGAAC CATGGTGGTG
    GACCGTGCAC TCTACCTCAG GAAGACTGTG CTTGGCAAGG TCATTTTCCA GGTCATCCTC
    GTGTTTGGAA TTCACTTCTG GATGTTCTTC ATCCTACCCA GTGTGACTGA GAGGAAATTC
    AGCCAGAACC TGGTGGCACA GCTCTGGTAC TTTGTGAAAT GTGTGTACTT TGGGTTGTCT
    GCCTACCAAA TCCGCTGTGG CTACCCAACA CGTGTGCTCG GAAACTTCCT CACGAAGAGC
    TACAATTATG TCAACCTCTT CTTGTTTCAA GGGTTTCGTC TCGTCCCATT TCTGACAGAG
    CTGCGGGCTG TGATGGACTG GGTGTGGACA GATACAACCT TGAGCCTCTC CAGCTGGATT
    TGTGTGGAGG ACATTTACGC ACACATTTTC ATCTTAAAGT GCTGGCGAGA GTCGGAAAAG
    AGATACCCTC AACCCAGGGG CCAGAAGAAG AAGAAAGCTG TGAAGTATGG CATGGGCGGA
    ATGATCATTG TCCTGCTCAT CTGTATCGTC TGGTTTCCTC TGCTCTTCAT GTCTTTGATC
    AAGTCTGTCG CAGGGGTTAT TAACCAACCC CTGGATGTGT CCGTCACAAT AACCCTGGGA
    GGCTATCAGC CTATCTTCAC AATGAGTGCC CAGCAAAGCC AGCTGAAAGT TATGGATGGT
    ACAAAGTATA ACGAATTCAT AAAGTCTTTC GGCCCCAACA GTGGCGCTAT GCAATTTCTG
    GAGAATTATG AAAGGCAAGA CATAACAGTA GCGGAACTGG AAGGGAATTC GAATTCTTTG
    TGGACCATCA GCCCTCCCAA AACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa54723
    Clone ID Related Accession (Same CDS sequence) XM_017587882.1 , XM_017601121.1
    Accession Version XM_017601121.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7887bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product piezo-type mechanosensitive ion channel component 2 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005117.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
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    301
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    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
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    2281
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    2641
    2701
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    2941
    3001
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    3181
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    4141
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    4261
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    4381
    4441
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    4681
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    5701
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    5881
    5941
    6001
    6061
    6121
    6181
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    6301
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    6421
    6481
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    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTCCCCAT CTGCCTGGCA 
    GTAGCATGTG CATTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCG ACGATGCAAG GACACACAGG GCGGCTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCCTTT CTGCTCCTTC ACATCATTTT CCACATCACA
    CTGGCCAGCC TGGAAGCTCA GCACCGCATC GCACCCGCTT ACAACTGCTC AACTTGGGAA
    AAGACCTTCC GGCAGATCGG CTTCGAAAGC TTGAAGGGAG CTGATGCTGG CAATGGCATC
    AGGGTGTTTG TGCCTGATAT CGGGATGTTC ATTGCGAGCC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACGGAA GAAGTAGCCC AATTTAATTC CGAGTGCGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GACTCAGAAG AAGCGCTGAT TTATGAGGAG
    GACTTAGATG GGGAAGAAGG CATGGAAGGC GAGCTGGAAG AAAGCACAAA GTTAAAAATG
    TTTCGCAGGC TCGCCTCCGT CGCTTCCAAG CTTAAGGAGT TCATCGGCAA TATGATAACC
    ACGGCGGGCA AGGTGGTGGT GACCATCCTG TTGGGCTCCT CTGGCATGAT GCTGCCATCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT CTGGGTCTGT GCACCTGGTG GTCCTGGTGC
    CGAACTTTTG ACCCATTGCT GTTTGGCTGC CTCTGTGTCC TGTTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTTCAGTTCT TCCAAGAAGC AGTCCCACCC
    AATGACTACT ATGCAAGGTT GTTTGGAATC AAATCCGTTA TTCAAACAGA CTGCTCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTACAC GCTGGCCACG CTGATCCGCA TTTGGTTGCA AGAGCCTCTT
    GTGCAGGAGG AGATGTCGAA GGAAGAGGAG GGTGCACTGG ATGGCTGCTC CAATCAGAAC
    ACAGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    CTTTTATCCA TGACCCAAGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACTGTGAAC
    GGCAACCCTG TGGACTACCA CACCATCCAC CCAAGTCTGC CCATAGAGAA TGGCCCCGCC
    AAGACGGACT TGTATACTAC TCCTCAGTAC CGGTGGGAGC CCTCAGAGGA GTCCTCAGAA
    AAGAAAGAGG AGGAAGAGGA CAAGAGAGAA GACTCAGAAG GGGAAGGGAG CCAAGAGGAA
    AAACGGAGTG TGAGAATTCA TGCCATGGTC GCCGTGTTCC AGTTCATCAT GAAGCAGAGT
    TACATCTGTG CCCTCATCGC CATGATGGCA TGGAGCATCA CCTACCACAG CTGGCTGACA
    TTTGTGCTGT TGATCTGGTC GTGCACTCTC TGGATGATTC GCAACAGGAG GAAGTACGCC
    ATGATCAGCT CTCCGTTCAT GGTTGTCTAT GCCAATCTGC TGCTGGTACT GCAGTACATA
    TGGAGCTTTG AACTTCCAGA AATCAAGAAA GTCCCTGGAT TTCTAGAAAA GAAAGAGCCT
    GGAGAGCTTG CCTCAAAGAT CCTCTTCACC ATCACATTTT GGCTGCTCCT GAGGCAGCAT
    CTCACAGAGC AGAAGGCCCT TCAGGAAAAG GAAGCACTTC TGTCTGAAGT CAAAATTGGA
    AGTCAAGAAC TTGACGAAAA GGATGAGGAA CTCCAAGATA TACAAGTGGA AGGAGAGCCC
    ATAGAGAAGG AAGAAGAGGA GGAGGAAATA AAGGAAGACA GACATGAGGT AAAGAAGGAA
    GAGGAAGAGG AAGAGGAAGA TGACGATGAC CAGGACATCA TGAAGGTTCT GGGCAATCTG
    GTGGTGGCCC TCTTCATCAA GTATTGGATC TACGTCTGCG GGGGCATGTT CTTTTTTGTC
    AGCTTTGAGG GGAAAATCGT CATGTACAAA ATCATCTACA TGGTGCTGTT CCTGTTCTGT
    GTGGCCTTGT ACCAGGTGCA CTACGAGTGG TGGAGGAAGA TCCTGAAATA CTTCTGGATG
    TCGGTGGTCA TTTACACCAT GCTGGTGCTC ATCTTCATCT ACACTTACCA GTTTGAGAAC
    TTCCCAGGCC TGTGGCAAAA CATGACCGGG TTAAAGAAAG AGAAGCTTGA GGATCTGGGC
    CTGAAGCAGT TCACTGTGGC CGAACTGTTC ACACGAATAT TCATCCCCAC CTCCTTTCTG
    CTGGTCTGCA TCCTGCACCT CCACTATTTC CATGACCGGT TCCTGGAACT CACAGACCTC
    AAGTCCATCC CCAGCAAAGA AGACAACACC ATCTACAGCC ATGCCAAGGT CAATGGTCGC
    GTGTACCTAA TAATAAATAG CATCAAGAAA AAATTACCAA TCCACCAGAA TGAGTTAGCC
    CACCCTGAAG GAAGCCTCCC AGACCTAGCC ATTATGAACA TGACGGCCAG CCTCGATAAG
    CCTGAGAGGC AGAAGTTGGC AGAGCCTGGG GAGGAGAGGA CAGAAGAATG TGCCAAGAAG
    GCAGAGAAGG GTGAGGCTGG GAAAGACAGT GATGAGTCTG AGGAGGAGGA AGGTGAGGAA
    GAGGAATCCG AGGAGGAAGA ATCCTCAGAT CTCAGGAACA AGTGGCACCT CGTGATTGAC
    CGGCTCACTG TGCTCTTTCT AAAGTTCCTG GAGTATTTCC ACAAGTTGCA GGTGTTTATG
    TGGTGGATCT TGGAGCTGCA CATCATCAAA ATCGTCTCAT CGTACATTAT CTGGGTGACT
    GTCAAAGAGG TGTCTCTGTT CAACTATGTA TTTTTGATTT CTTGGGCATT TGCTCTGCCA
    TATGCCAAAC TCCGCCGTGT GGCTTCCAGT GTCTGCACTG TCTGGACGTG TGTGATCATC
    GTCTGCAAAA TGTTGTACCA GCTGCAAACC ATTAAGCCTG AGAACTTTTC TGTCAACTGC
    TCCTTGCCCA ATGAAAATCA AACCAACATA CCCCTTAATG AGTTGAACAA GTCTCTTCTC
    TACAGTGCCC CTGTTGATCC CACTGAGTGG GTTGGCCTGA GGAAGTCCTC CCCTTTACTC
    GTCTACCTGA GGAACAACCT CCTGATGTTG GCGATTTTGG CCTTCGAAGT CACTGTTTAC
    CGACATCAGG AGTACTATCG AGGCCGAAAC AACTTGACTG CTCCGGTGTC TAAAACTATC
    TTTCATGACA TCACAAGGTT ACATCTAGAT GATGGGCTTA TCAATTGTGC CAAATATTTT
    GTAAACTACT TCTTCTACAA GTTTGGCCTA GAGACCTGTT TCCTAATGTC AGTGAACGTA
    ATTGGACAGC GAATGGACTT CTACGCCATG ATTCATGCCT GTTGGCTGAT CGGTGTCTTG
    TACCGTCGCA GAAGAAAAGC CATTGCTGAG GTCTGGCCCA AGTACTGCTG CTTCCTGGCT
    TGCATCATCA CCTTCCAGTA CTTTGTCTGC ATAGGCATCC CTCCTGCCCC CTGCCGAGAT
    TACCCATGGA GGTTCAAGGG TGCCAACTTC AATGACAACA TCATCAAGTG GCTGTACTTC
    CCAGATTTCA TCGTGCGGCC CAACCCTGTG TTTCTTGTCT ATGACTTCAT GCTGCTCCTG
    TGTGCTTCCT TACAACGACA GATTTTTGAG GACGAAAACA AGGCAGCTGT GCGCATCATG
    GCCGGGGACA ATGTCGAGAT TTGCATGAAC CTTGACGCGG CCTCATTCAG CCAGCATAAC
    CCTGTACCAG ATTTCATTCA CTGCAGATCT TACTTGGACA TGTCCAAAGT GATCATCTTC
    AGCTACCTCT TCTGGTTCGT CCTCACCATC ATCTTCATTA CCGGAACGAC CAGGATCAGC
    ATCTTTTGCA TGGGTTACCT GGTGGCCTGT TTCTATTTCC TGCTCTTTGG AGGTGATCTG
    CTCCTGAAAC CCATCAAGAG CATCCTACGC TACTGGGACT GGCTGATTGC ATACAACGTG
    TTTGTGATCA CCATGAAAAA CATACTGTCA ATAGGAGCCT GTGGATACAT CGGTGCACTG
    GTGAGAAACA GCTGCTGGCT GATCCAAGCG TTCAGCCTGG CCTGCACGGT GAAAGGCTAT
    AAAATGCCTG AAGATGACTC CAGATGCAAG CTGCCCAGTG GGGAGGCAGG GATCATCTGG
    GACAGCATCT GCTTTGCGTT TCTCCTGCTA CAGAGGAGAG TCTTCATGAG TTACTATTTC
    CTGCATGTCG TGGCTGACAT TAAAGCATCC CAGATCCTGG CATCCAGAGG GGCCGAACTT
    TTCCAGGCTA CAATTGTGAA AGCTGTAAAG GCAAGAATTG AAGAAGAGAA AAAATCCATG
    GACCAGCTGA AGAGACAGAT GGATCGCATC AAAGCCCGGC AGCAAAAGTA CAAAAAGGGT
    AAGGAGAGGA TGCTGAGCTT GACCCAGGAG TCAGGGGAAG GCCAGGACAT CCAGAAAGTC
    TCAGAAGAAG ACGATGAAAG AGAAGCAGAC AAACAGAAAG CCAAGGGCAA AAAAAAGCAG
    TGGTGGCGGC CTTGGGTTGA TCATGCTTCC ATGGTCAGGA GTGGAGATTA TTATTTGTTT
    GAAACGGATA GTGAAGAGGA GGAAGAAGAG GAACTCAAAA AGGAGGATGA AGAACCACCA
    CGGAAGTCTG CATTCCAGAG AGCTATTGGG AAGTTTGCCT CAGCCATTTT AGCCTTGCCA
    AAGTCTGTCA TTAAACTTCC CAAAACTATT CTTCAGTATT TAATCAGAGC TGCAAAGTTC
    GTCTACCAAG CCTGGATTAC CGATCCTAAA ACAGCACTCC GACAGAGGAG GAAAGAGAAG
    AAGAAAATGG CCAGAGAAGA GCAGAAGGGA AGACGCAAAG GATCTGGGGA GGGTCCAGTG
    GAATGGGAAG ACAGAGAAGA TGAGCCAATC AAAAAGAAAT CTGATGGACC AGATAATATC
    ATCAAGAGGA TATTTAATAT CCTGAAGTTC ACCTGGGTCC TGTTCCTGGC CACAGTGGAC
    AGTTTCACTA CTTGGCTCAA CTCCATCTCA AGAGAGCATA TCGACATATC CACAGTTCTG
    CGGATTGAAA GATGCATGCT GACCCGAGAG ATAAAGAAGG GCAACGTTCC GACACGGGAA
    AGCATCCACA TGTACTACCA GAACCACATG ATGAACCTTT CCAGAGAATC CGGACTGGAC
    ACAATCGACG AGCACTCTGG CGCTGGTTCC AGGGCCCAGG CAGCACACAG GATGGATAGC
    TTAGATTCCC ATGACAGTAT CTCCAGCTGT TACACTGAAG CAACCCTGCT GATCTCAAGG
    CAGTCCACCC TTGATGATTT AGATGGACAA GACCCTGTTC CTAAAACAAG CGAGCGTGCT
    CGGCCTAGGC TCCGTAAAGT GTTCAGCATG GACATGTCCT CCTCATCAGC TGACAGTGGC
    AGTGTAGCAT CAAGCGAGCC CACACAGTGC ACCATGCTGT ATTCACGCCA AGGAACTACG
    GAAACCATTG AGGAGGTAGA GGCTGAAGCT GAGGAGGAGG TGGTAGAGAG TCTGGAGCCT
    GAGCTCCATG ATGCCGAAGA GAAGGAATAT GCAGCTGACT ATGAAGCAGG GGTAGAGGAG
    GTCAGCCTCA CTCCAGATGA GGAGTTGCCT CAGTTCTCAA CAGATGACGG TGACGTGGAG
    GCCCCGCCCT CCTACAGCAA GGCTGTCAGC TTTGAGCACC TCTCGTTTGC ATCACAAGAT
    GACTCCGGGG GCAAGAACCA CATGGTGGTC AGCCCTGACG ACAGCCGCAC AGACAAGCTG
    GAGTCCAGTA TCTTACCGCC CCTGACTCAC GAGCTGACAG CCAGCGATCT GCTCATGAGC
    AAGATGTTCC GTGATGATGA GCTTGAAGAA TCTGAGAAAT TCTACGTGGA CCAGCCCCGG
    TTCTTGCTGC TATTCTATGC CATGTATAAC ACTCTGGTAG CCCGCTCGGA AATGGTGTGC
    TATTTCGTGA TCATCCTGAA CCACATGATC TCTGCCTCCA TCATTACTCT TCTGTTGCCC
    ATCCTGATCT TCCTCTGGGC CATGCTGTCC GTCCCCAGGC CCAGCCGGCG CTTCTGGATG
    ATGGCCATCG TCTACACTGA GGTGGCGATT GTAGTGAAAT ATTTCTTCCA GTTTGGCTTC
    TTTCCTTGGA ATAAGGACTT GGAAATTTAC AAGGACAGGC CCTACTTTCC ACCAAACATC
    ATAGGGGTGG AAAAGAAAGA GGGCTATGTT CTTTACGACC TCATTCAGCT GCTGGCTCTG
    TTCTTCCATC GATCCATTCT GAAGTGTCAT GGCCTATGGG ACGAGGATGA CATTGTTGAC
    AGTAACACAG ACAAGGAAGG ATCTGATGAT GAGCTGTCCC TCGGTCAGGG CAGAAGAGGC
    TCCTCAGAGT CACTGAAGTC CATCAACTTG GCTGCCTCTG TAGAGTCCGT CCATGTGACC
    TTCCCAGAGC AGCCAGCAGC CATCCGGAGA AAACGCTCCT GCAGCAGCTC GCAGATCTCC
    CCACGGTCCA GCTTCTCTTC AAATAGGTCC AAAAGAGGCA GCACAAGCAC CCGAAACAGC
    AGCCAAAAAG GAAGCAGTGT TTTGAGCATT AAACAAAAAA GCAAAAGAGA ACTTTACATG
    GAAAAGCTTC AAGAACATTT AATCAAAGCA AAGGCCTTTA CCATAAAGAA AACTCTACAA
    ATCTATGTAC CCATTCGGCA ATTCTTCTAT GACCTCATCC ACCCAGACTA CAGTGCTGTG
    ACTGACGTGT ATGTGCTGAT GTTCCTGGCC GACACCGTGG ACTTCATCAT CATCGTCTTT
    GGCTTCTGGG CTTTTGGGAA ACACTCGGCA GCTGCAGATA TCACCTCTTC CCTATCAGAA
    GACCAGGTCC CCGGGCCATT CCTGGTGATG GTTCTCATTC AGTTTGGAAC CATGGTGGTG
    GACCGTGCAC TCTACCTCAG GAAGACTGTG CTTGGCAAGG TCATTTTCCA GGTCATCCTC
    GTGTTTGGAA TTCACTTCTG GATGTTCTTC ATCCTACCCA GTGTGACTGA GAGGAAATTC
    AGCCAGAACC TGGTGGCACA GCTCTGGTAC TTTGTGAAAT GTGTGTACTT TGGGTTGTCT
    GCCTACCAAA TCCGCTGTGG CTACCCAACA CGTGTGCTCG GAAACTTCCT CACGAAGAGC
    TACAATTATG TCAACCTCTT CTTGTTTCAA GGGTTTCGTC TCGTCCCATT TCTGACAGAG
    CTGCGGGCTG TGATGGACTG GGTGTGGACA GATACAACCT TGAGCCTCTC CAGCTGGATT
    TGTGTGGAGG ACATTTACGC ACACATTTTC ATCTTAAAGT GCTGGCGAGA GTCGGAAAAG
    AGATACCCTC AACCCAGGGG CCAGAAGAAG AAGAAAGCTG TGAAGTATGG CATGGGCGGA
    ATGATCATTG TCCTGCTCAT CTGTATCGTC TGGTTTCCTC TGCTCTTCAT GTCTTTGATC
    AAGTCTGTCG CAGGGGTTAT TAACCAACCC CTGGATGTGT CCGTCACAAT AACCCTGGGA
    GGCTATCAGC CTATCTTCAC AATGAGTGCC CAGCAAAGCC AGCTGAAAGT TATGGATGGT
    ACAAAGTATA ACGAATTCAT AAAGTCTTTC GGCCCCAACA GTGGCGCTAT GCAATTTCTG
    GAGAATTATG AAAGGCAAGA CATAACAGTA GCGGAACTGG AAGGGAATTC GAATTCTTTG
    TGGACCATCA GCCCTCCCAA AACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017456610.1
    CDS943..8829
    Translation

    Target ORF information:

    RefSeq Version XM_017601121.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017601121.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTCCCCAT CTGCCTGGCA 
    GTAGCATGTG CATTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCG ACGATGCAAG GACACACAGG GCGGCTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCCTTT CTGCTCCTTC ACATCATTTT CCACATCACA
    CTGGCCAGCC TGGAAGCTCA GCACCGCATC GCACCCGCTT ACAACTGCTC AACTTGGGAA
    AAGACCTTCC GGCAGATCGG CTTCGAAAGC TTGAAGGGAG CTGATGCTGG CAATGGCATC
    AGGGTGTTTG TGCCTGATAT CGGGATGTTC ATTGCGAGCC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACGGAA GAAGTAGCCC AATTTAATTC CGAGTGCGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GACTCAGAAG AAGCGCTGAT TTATGAGGAG
    GACTTAGATG GGGAAGAAGG CATGGAAGGC GAGCTGGAAG AAAGCACAAA GTTAAAAATG
    TTTCGCAGGC TCGCCTCCGT CGCTTCCAAG CTTAAGGAGT TCATCGGCAA TATGATAACC
    ACGGCGGGCA AGGTGGTGGT GACCATCCTG TTGGGCTCCT CTGGCATGAT GCTGCCATCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT CTGGGTCTGT GCACCTGGTG GTCCTGGTGC
    CGAACTTTTG ACCCATTGCT GTTTGGCTGC CTCTGTGTCC TGTTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTTCAGTTCT TCCAAGAAGC AGTCCCACCC
    AATGACTACT ATGCAAGGTT GTTTGGAATC AAATCCGTTA TTCAAACAGA CTGCTCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTACAC GCTGGCCACG CTGATCCGCA TTTGGTTGCA AGAGCCTCTT
    GTGCAGGAGG AGATGTCGAA GGAAGAGGAG GGTGCACTGG ATGGCTGCTC CAATCAGAAC
    ACAGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    CTTTTATCCA TGACCCAAGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACTGTGAAC
    GGCAACCCTG TGGACTACCA CACCATCCAC CCAAGTCTGC CCATAGAGAA TGGCCCCGCC
    AAGACGGACT TGTATACTAC TCCTCAGTAC CGGTGGGAGC CCTCAGAGGA GTCCTCAGAA
    AAGAAAGAGG AGGAAGAGGA CAAGAGAGAA GACTCAGAAG GGGAAGGGAG CCAAGAGGAA
    AAACGGAGTG TGAGAATTCA TGCCATGGTC GCCGTGTTCC AGTTCATCAT GAAGCAGAGT
    TACATCTGTG CCCTCATCGC CATGATGGCA TGGAGCATCA CCTACCACAG CTGGCTGACA
    TTTGTGCTGT TGATCTGGTC GTGCACTCTC TGGATGATTC GCAACAGGAG GAAGTACGCC
    ATGATCAGCT CTCCGTTCAT GGTTGTCTAT GCCAATCTGC TGCTGGTACT GCAGTACATA
    TGGAGCTTTG AACTTCCAGA AATCAAGAAA GTCCCTGGAT TTCTAGAAAA GAAAGAGCCT
    GGAGAGCTTG CCTCAAAGAT CCTCTTCACC ATCACATTTT GGCTGCTCCT GAGGCAGCAT
    CTCACAGAGC AGAAGGCCCT TCAGGAAAAG GAAGCACTTC TGTCTGAAGT CAAAATTGGA
    AGTCAAGAAC TTGACGAAAA GGATGAGGAA CTCCAAGATA TACAAGTGGA AGGAGAGCCC
    ATAGAGAAGG AAGAAGAGGA GGAGGAAATA AAGGAAGACA GACATGAGGT AAAGAAGGAA
    GAGGAAGAGG AAGAGGAAGA TGACGATGAC CAGGACATCA TGAAGGTTCT GGGCAATCTG
    GTGGTGGCCC TCTTCATCAA GTATTGGATC TACGTCTGCG GGGGCATGTT CTTTTTTGTC
    AGCTTTGAGG GGAAAATCGT CATGTACAAA ATCATCTACA TGGTGCTGTT CCTGTTCTGT
    GTGGCCTTGT ACCAGGTGCA CTACGAGTGG TGGAGGAAGA TCCTGAAATA CTTCTGGATG
    TCGGTGGTCA TTTACACCAT GCTGGTGCTC ATCTTCATCT ACACTTACCA GTTTGAGAAC
    TTCCCAGGCC TGTGGCAAAA CATGACCGGG TTAAAGAAAG AGAAGCTTGA GGATCTGGGC
    CTGAAGCAGT TCACTGTGGC CGAACTGTTC ACACGAATAT TCATCCCCAC CTCCTTTCTG
    CTGGTCTGCA TCCTGCACCT CCACTATTTC CATGACCGGT TCCTGGAACT CACAGACCTC
    AAGTCCATCC CCAGCAAAGA AGACAACACC ATCTACAGCC ATGCCAAGGT CAATGGTCGC
    GTGTACCTAA TAATAAATAG CATCAAGAAA AAATTACCAA TCCACCAGAA TGAGTTAGCC
    CACCCTGAAG GAAGCCTCCC AGACCTAGCC ATTATGAACA TGACGGCCAG CCTCGATAAG
    CCTGAGAGGC AGAAGTTGGC AGAGCCTGGG GAGGAGAGGA CAGAAGAATG TGCCAAGAAG
    GCAGAGAAGG GTGAGGCTGG GAAAGACAGT GATGAGTCTG AGGAGGAGGA AGGTGAGGAA
    GAGGAATCCG AGGAGGAAGA ATCCTCAGAT CTCAGGAACA AGTGGCACCT CGTGATTGAC
    CGGCTCACTG TGCTCTTTCT AAAGTTCCTG GAGTATTTCC ACAAGTTGCA GGTGTTTATG
    TGGTGGATCT TGGAGCTGCA CATCATCAAA ATCGTCTCAT CGTACATTAT CTGGGTGACT
    GTCAAAGAGG TGTCTCTGTT CAACTATGTA TTTTTGATTT CTTGGGCATT TGCTCTGCCA
    TATGCCAAAC TCCGCCGTGT GGCTTCCAGT GTCTGCACTG TCTGGACGTG TGTGATCATC
    GTCTGCAAAA TGTTGTACCA GCTGCAAACC ATTAAGCCTG AGAACTTTTC TGTCAACTGC
    TCCTTGCCCA ATGAAAATCA AACCAACATA CCCCTTAATG AGTTGAACAA GTCTCTTCTC
    TACAGTGCCC CTGTTGATCC CACTGAGTGG GTTGGCCTGA GGAAGTCCTC CCCTTTACTC
    GTCTACCTGA GGAACAACCT CCTGATGTTG GCGATTTTGG CCTTCGAAGT CACTGTTTAC
    CGACATCAGG AGTACTATCG AGGCCGAAAC AACTTGACTG CTCCGGTGTC TAAAACTATC
    TTTCATGACA TCACAAGGTT ACATCTAGAT GATGGGCTTA TCAATTGTGC CAAATATTTT
    GTAAACTACT TCTTCTACAA GTTTGGCCTA GAGACCTGTT TCCTAATGTC AGTGAACGTA
    ATTGGACAGC GAATGGACTT CTACGCCATG ATTCATGCCT GTTGGCTGAT CGGTGTCTTG
    TACCGTCGCA GAAGAAAAGC CATTGCTGAG GTCTGGCCCA AGTACTGCTG CTTCCTGGCT
    TGCATCATCA CCTTCCAGTA CTTTGTCTGC ATAGGCATCC CTCCTGCCCC CTGCCGAGAT
    TACCCATGGA GGTTCAAGGG TGCCAACTTC AATGACAACA TCATCAAGTG GCTGTACTTC
    CCAGATTTCA TCGTGCGGCC CAACCCTGTG TTTCTTGTCT ATGACTTCAT GCTGCTCCTG
    TGTGCTTCCT TACAACGACA GATTTTTGAG GACGAAAACA AGGCAGCTGT GCGCATCATG
    GCCGGGGACA ATGTCGAGAT TTGCATGAAC CTTGACGCGG CCTCATTCAG CCAGCATAAC
    CCTGTACCAG ATTTCATTCA CTGCAGATCT TACTTGGACA TGTCCAAAGT GATCATCTTC
    AGCTACCTCT TCTGGTTCGT CCTCACCATC ATCTTCATTA CCGGAACGAC CAGGATCAGC
    ATCTTTTGCA TGGGTTACCT GGTGGCCTGT TTCTATTTCC TGCTCTTTGG AGGTGATCTG
    CTCCTGAAAC CCATCAAGAG CATCCTACGC TACTGGGACT GGCTGATTGC ATACAACGTG
    TTTGTGATCA CCATGAAAAA CATACTGTCA ATAGGAGCCT GTGGATACAT CGGTGCACTG
    GTGAGAAACA GCTGCTGGCT GATCCAAGCG TTCAGCCTGG CCTGCACGGT GAAAGGCTAT
    AAAATGCCTG AAGATGACTC CAGATGCAAG CTGCCCAGTG GGGAGGCAGG GATCATCTGG
    GACAGCATCT GCTTTGCGTT TCTCCTGCTA CAGAGGAGAG TCTTCATGAG TTACTATTTC
    CTGCATGTCG TGGCTGACAT TAAAGCATCC CAGATCCTGG CATCCAGAGG GGCCGAACTT
    TTCCAGGCTA CAATTGTGAA AGCTGTAAAG GCAAGAATTG AAGAAGAGAA AAAATCCATG
    GACCAGCTGA AGAGACAGAT GGATCGCATC AAAGCCCGGC AGCAAAAGTA CAAAAAGGGT
    AAGGAGAGGA TGCTGAGCTT GACCCAGGAG TCAGGGGAAG GCCAGGACAT CCAGAAAGTC
    TCAGAAGAAG ACGATGAAAG AGAAGCAGAC AAACAGAAAG CCAAGGGCAA AAAAAAGCAG
    TGGTGGCGGC CTTGGGTTGA TCATGCTTCC ATGGTCAGGA GTGGAGATTA TTATTTGTTT
    GAAACGGATA GTGAAGAGGA GGAAGAAGAG GAACTCAAAA AGGAGGATGA AGAACCACCA
    CGGAAGTCTG CATTCCAGAG AGCTATTGGG AAGTTTGCCT CAGCCATTTT AGCCTTGCCA
    AAGTCTGTCA TTAAACTTCC CAAAACTATT CTTCAGTATT TAATCAGAGC TGCAAAGTTC
    GTCTACCAAG CCTGGATTAC CGATCCTAAA ACAGCACTCC GACAGAGGAG GAAAGAGAAG
    AAGAAAATGG CCAGAGAAGA GCAGAAGGGA AGACGCAAAG GATCTGGGGA GGGTCCAGTG
    GAATGGGAAG ACAGAGAAGA TGAGCCAATC AAAAAGAAAT CTGATGGACC AGATAATATC
    ATCAAGAGGA TATTTAATAT CCTGAAGTTC ACCTGGGTCC TGTTCCTGGC CACAGTGGAC
    AGTTTCACTA CTTGGCTCAA CTCCATCTCA AGAGAGCATA TCGACATATC CACAGTTCTG
    CGGATTGAAA GATGCATGCT GACCCGAGAG ATAAAGAAGG GCAACGTTCC GACACGGGAA
    AGCATCCACA TGTACTACCA GAACCACATG ATGAACCTTT CCAGAGAATC CGGACTGGAC
    ACAATCGACG AGCACTCTGG CGCTGGTTCC AGGGCCCAGG CAGCACACAG GATGGATAGC
    TTAGATTCCC ATGACAGTAT CTCCAGCTGT TACACTGAAG CAACCCTGCT GATCTCAAGG
    CAGTCCACCC TTGATGATTT AGATGGACAA GACCCTGTTC CTAAAACAAG CGAGCGTGCT
    CGGCCTAGGC TCCGTAAAGT GTTCAGCATG GACATGTCCT CCTCATCAGC TGACAGTGGC
    AGTGTAGCAT CAAGCGAGCC CACACAGTGC ACCATGCTGT ATTCACGCCA AGGAACTACG
    GAAACCATTG AGGAGGTAGA GGCTGAAGCT GAGGAGGAGG TGGTAGAGAG TCTGGAGCCT
    GAGCTCCATG ATGCCGAAGA GAAGGAATAT GCAGCTGACT ATGAAGCAGG GGTAGAGGAG
    GTCAGCCTCA CTCCAGATGA GGAGTTGCCT CAGTTCTCAA CAGATGACGG TGACGTGGAG
    GCCCCGCCCT CCTACAGCAA GGCTGTCAGC TTTGAGCACC TCTCGTTTGC ATCACAAGAT
    GACTCCGGGG GCAAGAACCA CATGGTGGTC AGCCCTGACG ACAGCCGCAC AGACAAGCTG
    GAGTCCAGTA TCTTACCGCC CCTGACTCAC GAGCTGACAG CCAGCGATCT GCTCATGAGC
    AAGATGTTCC GTGATGATGA GCTTGAAGAA TCTGAGAAAT TCTACGTGGA CCAGCCCCGG
    TTCTTGCTGC TATTCTATGC CATGTATAAC ACTCTGGTAG CCCGCTCGGA AATGGTGTGC
    TATTTCGTGA TCATCCTGAA CCACATGATC TCTGCCTCCA TCATTACTCT TCTGTTGCCC
    ATCCTGATCT TCCTCTGGGC CATGCTGTCC GTCCCCAGGC CCAGCCGGCG CTTCTGGATG
    ATGGCCATCG TCTACACTGA GGTGGCGATT GTAGTGAAAT ATTTCTTCCA GTTTGGCTTC
    TTTCCTTGGA ATAAGGACTT GGAAATTTAC AAGGACAGGC CCTACTTTCC ACCAAACATC
    ATAGGGGTGG AAAAGAAAGA GGGCTATGTT CTTTACGACC TCATTCAGCT GCTGGCTCTG
    TTCTTCCATC GATCCATTCT GAAGTGTCAT GGCCTATGGG ACGAGGATGA CATTGTTGAC
    AGTAACACAG ACAAGGAAGG ATCTGATGAT GAGCTGTCCC TCGGTCAGGG CAGAAGAGGC
    TCCTCAGAGT CACTGAAGTC CATCAACTTG GCTGCCTCTG TAGAGTCCGT CCATGTGACC
    TTCCCAGAGC AGCCAGCAGC CATCCGGAGA AAACGCTCCT GCAGCAGCTC GCAGATCTCC
    CCACGGTCCA GCTTCTCTTC AAATAGGTCC AAAAGAGGCA GCACAAGCAC CCGAAACAGC
    AGCCAAAAAG GAAGCAGTGT TTTGAGCATT AAACAAAAAA GCAAAAGAGA ACTTTACATG
    GAAAAGCTTC AAGAACATTT AATCAAAGCA AAGGCCTTTA CCATAAAGAA AACTCTACAA
    ATCTATGTAC CCATTCGGCA ATTCTTCTAT GACCTCATCC ACCCAGACTA CAGTGCTGTG
    ACTGACGTGT ATGTGCTGAT GTTCCTGGCC GACACCGTGG ACTTCATCAT CATCGTCTTT
    GGCTTCTGGG CTTTTGGGAA ACACTCGGCA GCTGCAGATA TCACCTCTTC CCTATCAGAA
    GACCAGGTCC CCGGGCCATT CCTGGTGATG GTTCTCATTC AGTTTGGAAC CATGGTGGTG
    GACCGTGCAC TCTACCTCAG GAAGACTGTG CTTGGCAAGG TCATTTTCCA GGTCATCCTC
    GTGTTTGGAA TTCACTTCTG GATGTTCTTC ATCCTACCCA GTGTGACTGA GAGGAAATTC
    AGCCAGAACC TGGTGGCACA GCTCTGGTAC TTTGTGAAAT GTGTGTACTT TGGGTTGTCT
    GCCTACCAAA TCCGCTGTGG CTACCCAACA CGTGTGCTCG GAAACTTCCT CACGAAGAGC
    TACAATTATG TCAACCTCTT CTTGTTTCAA GGGTTTCGTC TCGTCCCATT TCTGACAGAG
    CTGCGGGCTG TGATGGACTG GGTGTGGACA GATACAACCT TGAGCCTCTC CAGCTGGATT
    TGTGTGGAGG ACATTTACGC ACACATTTTC ATCTTAAAGT GCTGGCGAGA GTCGGAAAAG
    AGATACCCTC AACCCAGGGG CCAGAAGAAG AAGAAAGCTG TGAAGTATGG CATGGGCGGA
    ATGATCATTG TCCTGCTCAT CTGTATCGTC TGGTTTCCTC TGCTCTTCAT GTCTTTGATC
    AAGTCTGTCG CAGGGGTTAT TAACCAACCC CTGGATGTGT CCGTCACAAT AACCCTGGGA
    GGCTATCAGC CTATCTTCAC AATGAGTGCC CAGCAAAGCC AGCTGAAAGT TATGGATGGT
    ACAAAGTATA ACGAATTCAT AAAGTCTTTC GGCCCCAACA GTGGCGCTAT GCAATTTCTG
    GAGAATTATG AAAGGCAAGA CATAACAGTA GCGGAACTGG AAGGGAATTC GAATTCTTTG
    TGGACCATCA GCCCTCCCAA AACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa20913
    Clone ID Related Accession (Same CDS sequence) XM_006254839.3 , XM_006222595.3
    Accession Version XM_006254839.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8469bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product piezo-type mechanosensitive ion channel component 2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005117.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006254839.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTCCCCAT CTGCCTGGCA 
    GTAGCATGTG CATTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCG ACGATGCAAG GACACACAGG GCGGCTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCCTTT CTGCTCCTTC ACATCATTTT CCACATCACA
    CTGGCCAGCC TGGAAGCTCA GCACCGCATC GCACCCGCTT ACAACTGCTC AACTTGGGAA
    AAGACCTTCC GGCAGATCGG CTTCGAAAGC TTGAAGGGAG CTGATGCTGG CAATGGCATC
    AGGGTGTTTG TGCCTGATAT CGGGATGTTC ATTGCGAGCC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACGGAA GAAGTAGCCC AATTTAATTC CGAGTGCGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GACTCAGAAG AAGCGCTGAT TTATGAGGAG
    GACTTAGATG GGGAAGAAGG CATGGAAGGC GAGCTGGAAG AAAGCACAAA GTTAAAAATG
    TTTCGCAGGC TCGCCTCCGT CGCTTCCAAG CTTAAGGAGT TCATCGGCAA TATGATAACC
    ACGGCGGGCA AGGTGGTGGT GACCATCCTG TTGGGCTCCT CTGGCATGAT GCTGCCATCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT CTGGGTCTGT GCACCTGGTG GTCCTGGTGC
    CGAACTTTTG ACCCATTGCT GTTTGGCTGC CTCTGTGTCC TGTTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTTCAGTTCT TCCAAGAAGC AGTCCCACCC
    AATGACTACT ATGCAAGGTT GTTTGGAATC AAATCCGTTA TTCAAACAGA CTGCTCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTACAC GCTGGCCACG CTGATCCGCA TTTGGTTGCA AGAGCCTCTT
    GTGCAGGAGG AGATGTCGAA GGAAGAGGAG GGTGCACTGG ATGGCTGCTC CAATCAGAAC
    ACAGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    CTTTTATCCA TGACCCAAGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACTGTGAAC
    GGCAACCCTG TGGACTACCA CACCATCCAC CCAAGTCTGC CCATAGAGAA TGGCCCCGCC
    AAGACGGACT TGTATACTAC TCCTCAGTAC CGGTGGGAGC CCTCAGAGGA GTCCTCAGAA
    AAGAAAGAGG AGGAAGAGGA CAAGAGAGAA GACTCAGAAG GGGAAGGGAG CCAAGAGGAA
    AAACGGAGTG TGAGAATTCA TGCCATGGTC GCCGTGTTCC AGTTCATCAT GAAGCAGAGT
    TACATCTGTG CCCTCATCGC CATGATGGCA TGGAGCATCA CCTACCACAG CTGGCTGACA
    TTTGTGCTGT TGATCTGGTC GTGCACTCTC TGGATGATTC GCAACAGGAG GAAGTACGCC
    ATGATCAGCT CTCCGTTCAT GGTTGTCTAT GCCAATCTGC TGCTGGTACT GCAGTACATA
    TGGAGCTTTG AACTTCCAGA AATCAAGAAA GTCCCTGGAT TTCTAGAAAA GAAAGAGCCT
    GGAGAGCTTG CCTCAAAGAT CCTCTTCACC ATCACATTTT GGCTGCTCCT GAGGCAGCAT
    CTCACAGAGC AGAAGGCCCT TCAGGAAAAG GAAGCACTTC TGTCTGAAGT CAAAATTGGA
    AGTCAAGAAC TTGACGAAAA GGATGAGGAA CTCCAAGATA TACAAGTGGA AGGAGAGCCC
    ATAGAGAAGG AAGAAGAGGA GGAGGAAATA AAGGAAGACA GACATGAGGT AAAGAAGGAA
    GAGGAAGAGG AAGAGGAAGA TGACGATGAC CAGGACATCA TGAAGGTTCT GGGCAATCTG
    GTGGTGGCCC TCTTCATCAA GTATTGGATC TACGTCTGCG GGGGCATGTT CTTTTTTGTC
    AGCTTTGAGG GGAAAATCGT CATGTACAAA ATCATCTACA TGGTGCTGTT CCTGTTCTGT
    GTGGCCTTGT ACCAGGTGCA CTACGAGTGG TGGAGGAAGA TCCTGAAATA CTTCTGGATG
    TCGGTGGTCA TTTACACCAT GCTGGTGCTC ATCTTCATCT ACACTTACCA GTTTGAGAAC
    TTCCCAGGCC TGTGGCAAAA CATGACCGGG TTAAAGAAAG AGAAGCTTGA GGATCTGGGC
    CTGAAGCAGT TCACTGTGGC CGAACTGTTC ACACGAATAT TCATCCCCAC CTCCTTTCTG
    CTGGTCTGCA TCCTGCACCT CCACTATTTC CATGACCGGT TCCTGGAACT CACAGACCTC
    AAGTCCATCC CCAGCAAAGA AGACAACACC ATCTACAGCC ATGCCAAGGT CAATGGTCGC
    GTGTACCTAA TAATAAATAG GTTAGCCCAC CCTGAAGGAA GCCTCCCAGA CCTAGCCATT
    ATGAACATGA CGGCCAGCCT CGATAAGCCT GAGAGGCAGA AGTTGGCAGA GCCTGGGGAG
    GAGAGGACAG AAGAATGTGC CAAGAAGGCA GAGAAGGGTG AGGCTGGGAA AGACAGTGAT
    GAGTCTGAGG AGGAGGAAGG TGAGGAAGAG GAATCCGAGG AGGAAGAATC CTCAGATCTC
    AGGAACAAGT GGCACCTCGT GATTGACCGG CTCACTGTGC TCTTTCTAAA GTTCCTGGAG
    TATTTCCACA AGTTGCAGGT GTTTATGTGG TGGATCTTGG AGCTGCACAT CATCAAAATC
    GTCTCATCGT ACATTATCTG GGTGACTGTC AAAGAGGTGT CTCTGTTCAA CTATGTATTT
    TTGATTTCTT GGGCATTTGC TCTGCCATAT GCCAAACTCC GCCGTGTGGC TTCCAGTGTC
    TGCACTGTCT GGACGTGTGT GATCATCGTC TGCAAAATGT TGTACCAGCT GCAAACCATT
    AAGCCTGAGA ACTTTTCTGT CAACTGCTCC TTGCCCAATG AAAATCAAAC CAACATACCC
    CTTAATGAGT TGAACAAGTC TCTTCTCTAC AGTGCCCCTG TTGATCCCAC TGAGTGGGTT
    GGCCTGAGGA AGTCCTCCCC TTTACTCGTC TACCTGAGGA ACAACCTCCT GATGTTGGCG
    ATTTTGGCCT TCGAAGTCAC TGTTTACCGA CATCAGGAGT ACTATCGAGG CCGAAACAAC
    TTGACTGCTC CGGTGTCTAA AACTATCTTT CATGACATCA CAAGGTTACA TCTAGATGAT
    GGGCTTATCA ATTGTGCCAA ATATTTTGTA AACTACTTCT TCTACAAGTT TGGCCTAGAG
    ACCTGTTTCC TAATGTCAGT GAACGTAATT GGACAGCGAA TGGACTTCTA CGCCATGATT
    CATGCCTGTT GGCTGATCGG TGTCTTGTAC CGTCGCAGAA GAAAAGCCAT TGCTGAGGTC
    TGGCCCAAGT ACTGCTGCTT CCTGGCTTGC ATCATCACCT TCCAGTACTT TGTCTGCATA
    GGCATCCCTC CTGCCCCCTG CCGAGATTAC CCATGGAGGT TCAAGGGTGC CAACTTCAAT
    GACAACATCA TCAAGTGGCT GTACTTCCCA GATTTCATCG TGCGGCCCAA CCCTGTGTTT
    CTTGTCTATG ACTTCATGCT GCTCCTGTGT GCTTCCTTAC AACGACAGAT TTTTGAGGAC
    GAAAACAAGG CAGCTGTGCG CATCATGGCC GGGGACAATG TCGAGATTTG CATGAACCTT
    GACGCGGCCT CATTCAGCCA GCATAACCCT GTACCAGATT TCATTCACTG CAGATCTTAC
    TTGGACATGT CCAAAGTGAT CATCTTCAGC TACCTCTTCT GGTTCGTCCT CACCATCATC
    TTCATTACCG GAACGACCAG GATCAGCATC TTTTGCATGG GTTACCTGGT GGCCTGTTTC
    TATTTCCTGC TCTTTGGAGG TGATCTGCTC CTGAAACCCA TCAAGAGCAT CCTACGCTAC
    TGGGACTGGC TGATTGCATA CAACGTGTTT GTGATCACCA TGAAAAACAT ACTGTCAATA
    GGAGCCTGTG GATACATCGG TGCACTGGTG AGAAACAGCT GCTGGCTGAT CCAAGCGTTC
    AGCCTGGCCT GCACGGTGAA AGGCTATAAA ATGCCTGAAG ATGACTCCAG ATGCAAGCTG
    CCCAGTGGGG AGGCAGGGAT CATCTGGGAC AGCATCTGCT TTGCGTTTCT CCTGCTACAG
    AGGAGAGTCT TCATGAGTTA CTATTTCCTG CATGTCGTGG CTGACATTAA AGCATCCCAG
    ATCCTGGCAT CCAGAGGGGC CGAACTTTTC CAGGCTACAA TTGTGAAAGC TGTAAAGGCA
    AGAATTGAAG AAGAGAAAAA ATCCATGGAC CAGCTGAAGA GACAGATGGA TCGCATCAAA
    GCCCGGCAGC AAAAGTACAA AAAGGGTAAG GAGAGGATGC TGAGCTTGAC CCAGGAGTCA
    GGGGAAGGCC AGGACATCCA GAAAGTCTCA GAAGAAGACG ATGAAAGAGA AGCAGACAAA
    CAGAAAGCCA AGGGCAAAAA AAAGCAGTGG TGGCGGCCTT GGGTTGATCA TGCTTCCATG
    GTCAGGAGTG GAGATTATTA TTTGTTTGAA ACGGATAGTG AAGAGGAGGA AGAAGAGGAA
    CTCAAAAAGG AGGATGAAGA ACCACCACGG AAGTCTGCAT TCCAGTTCGT CTACCAAGCC
    TGGATTACCG ATCCTAAAAC AGCACTCCGA CAGAGGAGGA AAGAGAAGAA GAAAATGGCC
    AGAGAAGAGC AGAAGGGAAG ACGCAAAGGA TCTGGGGAGG GTCCAGTGGA ATGGGAAGAC
    AGAGAAGATG AGCCAATCAA AAAGAAATCT GATGGACCAG ATAATATCAT CAAGAGGATA
    TTTAATATCC TGAAGTTCAC CTGGGTCCTG TTCCTGGCCA CAGTGGACAG TTTCACTACT
    TGGCTCAACT CCATCTCAAG AGAGCATATC GACATATCCA CAGTTCTGCG GATTGAAAGA
    TGCATGCTGA CCCGAGAGAT AAAGAAGGGC AACGTTCCGA CACGGGAAAG CATCCACATG
    TACTACCAGA ACCACATGAT GAACCTTTCC AGAGAATCCG GACTGGACAC AATCGACGAG
    CACTCTGGCG CTGGTTCCAG GGCCCAGGCA GCACACAGGA TGGATAGCTT AGATTCCCAT
    GACAGTATCT CCAGCTGTTA CACTGAAGCA ACCCTGCTGA TCTCAAGGCA GTCCACCCTT
    GATGATTTAG ATGGACAAGA CCCTGTTCCT AAAACAAGCG AGCGTGCTCG GCCTAGGCTC
    CGTAAAGTGT TCAGCATGGA CATGTCCTCC TCATCAGCTG ACAGTGGCAG TGTAGCATCA
    AGCGAGCCCA CACAGTGCAC CATGCTGTAT TCACGCCAAG GAACTACGGA AACCATTGAG
    GAGGTAGAGG CTGAAGCTGA GGAGGAGGTG GTAGAGAGTC TGGAGCCTGA GCTCCATGAT
    GCCGAAGAGA AGGAATATGC AGCTGACTAT GAAGCAGGGG TAGAGGAGGT CAGCCTCACT
    CCAGATGAGG AGTTGCCTCA GTTCTCAACA GATGACGGTG ACGTGGAGGC CCCGCCCTCC
    TACAGCAAGG CTGTCAGCTT TGAGCACCTC TCGTTTGCAT CACAAGATGA CTCCGGGGGC
    AAGAACCACA TGGTGGTCAG CCCTGACGAC AGCCGCACAG ACAAGCTGGA GTCCAGTATC
    TTACCGCCCC TGACTCACGA GCTGACAGCC AGCGATCTGC TCATGAGCAA GATGTTCCGT
    GATGATGAGC TTGAAGAATC TGAGAAATTC TACGTGGACC AGCCCCGGTT CTTGCTGCTA
    TTCTATGCCA TGTATAACAC TCTGGTAGCC CGCTCGGAAA TGGTGTGCTA TTTCGTGATC
    ATCCTGAACC ACATGATCTC TGCCTCCATC ATTACTCTTC TGTTGCCCAT CCTGATCTTC
    CTCTGGGCCA TGCTGTCCGT CCCCAGGCCC AGCCGGCGCT TCTGGATGAT GGCCATCGTC
    TACACTGAGG TGGCGATTGT AGTGAAATAT TTCTTCCAGT TTGGCTTCTT TCCTTGGAAT
    AAGGACTTGG AAATTTACAA GGACAGGCCC TACTTTCCAC CAAACATCAT AGGGGTGGAA
    AAGAAAGAGG GCTATGTTCT TTACGACCTC ATTCAGCTGC TGGCTCTGTT CTTCCATCGA
    TCCATTCTGA AGTGTCATGG CCTATGGGAC GAGGATGACA TTGTTGACAG TAACACAGAC
    AAGGAAGGAT CTGATGATGA GCTGTCCCTC GGTCAGGGCA GAAGAGGCTC CTCAGAGTCA
    CTGAAGTCCA TCAACTTGGC TGCCTCTGTA GAGTCCGTCC ATGTGACCTT CCCAGAGCAG
    CCAGCAGCCA TCCGGAGAAA ACGCTCCTGC AGCAGCTCGC AGATCTCCCC ACGGTCCAGC
    TTCTCTTCAA ATAGGTCCAA AAGAGGCAGC ACAAGCACCC GAAACAGCAG CCAAAAAGGA
    AGCAGTGTTT TGAGCATTAA ACAAAAAAGC AAAAGAGAAC TTTACATGGA AAAGCTTCAA
    GAACATTTAA TCAAAGCAAA GGCCTTTACC ATAAAGAAAA CTCTACAAAT CTATGTACCC
    ATTCGGCAAT TCTTCTATGA CCTCATCCAC CCAGACTACA GTGCTGTGAC TGACGTGTAT
    GTGCTGATGT TCCTGGCCGA CACCGTGGAC TTCATCATCA TCGTCTTTGG CTTCTGGGCT
    TTTGGGAAAC ACTCGGCAGC TGCAGATATC ACCTCTTCCC TATCAGAAGA CCAGGTCCCC
    GGGCCATTCC TGGTGATGGT TCTCATTCAG TTTGGAACCA TGGTGGTGGA CCGTGCACTC
    TACCTCAGGA AGACTGTGCT TGGCAAGGTC ATTTTCCAGG TCATCCTCGT GTTTGGAATT
    CACTTCTGGA TGTTCTTCAT CCTACCCAGT GTGACTGAGA GGAAATTCAG CCAGAACCTG
    GTGGCACAGC TCTGGTACTT TGTGAAATGT GTGTACTTTG GGTTGTCTGC CTACCAAATC
    CGCTGTGGCT ACCCAACACG TGTGCTCGGA AACTTCCTCA CGAAGAGCTA CAATTATGTC
    AACCTCTTCT TGTTTCAAGG GTTTCGTCTC GTCCCATTTC TGACAGAGCT GCGGGCTGTG
    ATGGACTGGG TGTGGACAGA TACAACCTTG AGCCTCTCCA GCTGGATTTG TGTGGAGGAC
    ATTTACGCAC ACATTTTCAT CTTAAAGTGC TGGCGAGAGT CGGAAAAGAG ATACCCTCAA
    CCCAGGGGCC AGAAGAAGAA GAAAGCTGTG AAGTATGGCA TGGGCGGAAT GATCATTGTC
    CTGCTCATCT GTATCGTCTG GTTTCCTCTG CTCTTCATGT CTTTGATCAA GTCTGTCGCA
    GGGGTTATTA ACCAACCCCT GGATGTGTCC GTCACAATAA CCCTGGGAGG CTATCAGCCT
    ATCTTCACAA TGAGTGCCCA GCAAAGCCAG CTGAAAGTTA TGGATGGTAC AAAGTATAAC
    GAATTCATAA AGTCTTTCGG CCCCAACAGT GGCGCTATGC AATTTCTGGA GAATTATGAA
    AGGCAAGACA TAACAGTAGC GGAACTGGAA GGGAATTCGA ATTCTTTGTG GACCATCAGC
    CCTCCCAGTA AGCAGAAAAT GATACAGGAG CTCACCGACC CCAATAGTTG CTTCTCTGTT
    GTTTTTTCAT GGAGTATCCA GAGAAACATG ACTCTGGGTG CAAAGGCTGA AATAGCAACG
    GACAAGCTTT CTTTTCCTCT CACGGATGCG ACACGAAGTA GCATAGCCAA AATGATTGCT
    GGCAATGACA CAGAAAGTTC CAACACACCA GTGACAATAG AAAAGATCTA CCCATACTAT
    GTGAAGGCAC CCAGTGACTC AAACTCAAAG CCTATAAAAC AGCTTCTGTC AGAAAACAAT
    TTCATGAATA TCACCATCAT TTTGTTCAGA GACAATGTCA CAAAGTCCAA TAGTGAGTGG
    TGGGTTCTCA ACCTGACTGG AAGTAGGATA TTCAATCAGG GATCCCAAGC CTTGGAGCTG
    GTGGTCTTCA ATGACAAAGT CAGCCCCCCA AGTCTGGGCT TCTTGGCTGG CTATGGCATC
    ATGGGATTGT ATGCATCCGT TGTCCTTGTA ATTGGGAAGT TTGTTCGCGA ATTCTTCAGT
    GGGATTTCTC ACTCTATCAT GTTTGAAGAG CTTCCAAATG TGGACAGAAT TTTGAAGTTA
    TGCACAGATA TATTTCTCGT ACGGGAGACA GGGGAACTGG AGCTGGAGGA AGACCTCTAT
    GCCAAATTGA TATTCCTGTA CAGGTCGCCA GAAACCATGA TCAAGTGGAC TAGAGAAAAA
    ACAAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006254901.1
    CDS940..9408
    Translation

    Target ORF information:

    RefSeq Version XM_006254839.3
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006254839.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTCCCCAT CTGCCTGGCA 
    GTAGCATGTG CATTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCG ACGATGCAAG GACACACAGG GCGGCTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCCTTT CTGCTCCTTC ACATCATTTT CCACATCACA
    CTGGCCAGCC TGGAAGCTCA GCACCGCATC GCACCCGCTT ACAACTGCTC AACTTGGGAA
    AAGACCTTCC GGCAGATCGG CTTCGAAAGC TTGAAGGGAG CTGATGCTGG CAATGGCATC
    AGGGTGTTTG TGCCTGATAT CGGGATGTTC ATTGCGAGCC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACGGAA GAAGTAGCCC AATTTAATTC CGAGTGCGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GACTCAGAAG AAGCGCTGAT TTATGAGGAG
    GACTTAGATG GGGAAGAAGG CATGGAAGGC GAGCTGGAAG AAAGCACAAA GTTAAAAATG
    TTTCGCAGGC TCGCCTCCGT CGCTTCCAAG CTTAAGGAGT TCATCGGCAA TATGATAACC
    ACGGCGGGCA AGGTGGTGGT GACCATCCTG TTGGGCTCCT CTGGCATGAT GCTGCCATCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT CTGGGTCTGT GCACCTGGTG GTCCTGGTGC
    CGAACTTTTG ACCCATTGCT GTTTGGCTGC CTCTGTGTCC TGTTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTTCAGTTCT TCCAAGAAGC AGTCCCACCC
    AATGACTACT ATGCAAGGTT GTTTGGAATC AAATCCGTTA TTCAAACAGA CTGCTCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTACAC GCTGGCCACG CTGATCCGCA TTTGGTTGCA AGAGCCTCTT
    GTGCAGGAGG AGATGTCGAA GGAAGAGGAG GGTGCACTGG ATGGCTGCTC CAATCAGAAC
    ACAGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    CTTTTATCCA TGACCCAAGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACTGTGAAC
    GGCAACCCTG TGGACTACCA CACCATCCAC CCAAGTCTGC CCATAGAGAA TGGCCCCGCC
    AAGACGGACT TGTATACTAC TCCTCAGTAC CGGTGGGAGC CCTCAGAGGA GTCCTCAGAA
    AAGAAAGAGG AGGAAGAGGA CAAGAGAGAA GACTCAGAAG GGGAAGGGAG CCAAGAGGAA
    AAACGGAGTG TGAGAATTCA TGCCATGGTC GCCGTGTTCC AGTTCATCAT GAAGCAGAGT
    TACATCTGTG CCCTCATCGC CATGATGGCA TGGAGCATCA CCTACCACAG CTGGCTGACA
    TTTGTGCTGT TGATCTGGTC GTGCACTCTC TGGATGATTC GCAACAGGAG GAAGTACGCC
    ATGATCAGCT CTCCGTTCAT GGTTGTCTAT GCCAATCTGC TGCTGGTACT GCAGTACATA
    TGGAGCTTTG AACTTCCAGA AATCAAGAAA GTCCCTGGAT TTCTAGAAAA GAAAGAGCCT
    GGAGAGCTTG CCTCAAAGAT CCTCTTCACC ATCACATTTT GGCTGCTCCT GAGGCAGCAT
    CTCACAGAGC AGAAGGCCCT TCAGGAAAAG GAAGCACTTC TGTCTGAAGT CAAAATTGGA
    AGTCAAGAAC TTGACGAAAA GGATGAGGAA CTCCAAGATA TACAAGTGGA AGGAGAGCCC
    ATAGAGAAGG AAGAAGAGGA GGAGGAAATA AAGGAAGACA GACATGAGGT AAAGAAGGAA
    GAGGAAGAGG AAGAGGAAGA TGACGATGAC CAGGACATCA TGAAGGTTCT GGGCAATCTG
    GTGGTGGCCC TCTTCATCAA GTATTGGATC TACGTCTGCG GGGGCATGTT CTTTTTTGTC
    AGCTTTGAGG GGAAAATCGT CATGTACAAA ATCATCTACA TGGTGCTGTT CCTGTTCTGT
    GTGGCCTTGT ACCAGGTGCA CTACGAGTGG TGGAGGAAGA TCCTGAAATA CTTCTGGATG
    TCGGTGGTCA TTTACACCAT GCTGGTGCTC ATCTTCATCT ACACTTACCA GTTTGAGAAC
    TTCCCAGGCC TGTGGCAAAA CATGACCGGG TTAAAGAAAG AGAAGCTTGA GGATCTGGGC
    CTGAAGCAGT TCACTGTGGC CGAACTGTTC ACACGAATAT TCATCCCCAC CTCCTTTCTG
    CTGGTCTGCA TCCTGCACCT CCACTATTTC CATGACCGGT TCCTGGAACT CACAGACCTC
    AAGTCCATCC CCAGCAAAGA AGACAACACC ATCTACAGCC ATGCCAAGGT CAATGGTCGC
    GTGTACCTAA TAATAAATAG GTTAGCCCAC CCTGAAGGAA GCCTCCCAGA CCTAGCCATT
    ATGAACATGA CGGCCAGCCT CGATAAGCCT GAGAGGCAGA AGTTGGCAGA GCCTGGGGAG
    GAGAGGACAG AAGAATGTGC CAAGAAGGCA GAGAAGGGTG AGGCTGGGAA AGACAGTGAT
    GAGTCTGAGG AGGAGGAAGG TGAGGAAGAG GAATCCGAGG AGGAAGAATC CTCAGATCTC
    AGGAACAAGT GGCACCTCGT GATTGACCGG CTCACTGTGC TCTTTCTAAA GTTCCTGGAG
    TATTTCCACA AGTTGCAGGT GTTTATGTGG TGGATCTTGG AGCTGCACAT CATCAAAATC
    GTCTCATCGT ACATTATCTG GGTGACTGTC AAAGAGGTGT CTCTGTTCAA CTATGTATTT
    TTGATTTCTT GGGCATTTGC TCTGCCATAT GCCAAACTCC GCCGTGTGGC TTCCAGTGTC
    TGCACTGTCT GGACGTGTGT GATCATCGTC TGCAAAATGT TGTACCAGCT GCAAACCATT
    AAGCCTGAGA ACTTTTCTGT CAACTGCTCC TTGCCCAATG AAAATCAAAC CAACATACCC
    CTTAATGAGT TGAACAAGTC TCTTCTCTAC AGTGCCCCTG TTGATCCCAC TGAGTGGGTT
    GGCCTGAGGA AGTCCTCCCC TTTACTCGTC TACCTGAGGA ACAACCTCCT GATGTTGGCG
    ATTTTGGCCT TCGAAGTCAC TGTTTACCGA CATCAGGAGT ACTATCGAGG CCGAAACAAC
    TTGACTGCTC CGGTGTCTAA AACTATCTTT CATGACATCA CAAGGTTACA TCTAGATGAT
    GGGCTTATCA ATTGTGCCAA ATATTTTGTA AACTACTTCT TCTACAAGTT TGGCCTAGAG
    ACCTGTTTCC TAATGTCAGT GAACGTAATT GGACAGCGAA TGGACTTCTA CGCCATGATT
    CATGCCTGTT GGCTGATCGG TGTCTTGTAC CGTCGCAGAA GAAAAGCCAT TGCTGAGGTC
    TGGCCCAAGT ACTGCTGCTT CCTGGCTTGC ATCATCACCT TCCAGTACTT TGTCTGCATA
    GGCATCCCTC CTGCCCCCTG CCGAGATTAC CCATGGAGGT TCAAGGGTGC CAACTTCAAT
    GACAACATCA TCAAGTGGCT GTACTTCCCA GATTTCATCG TGCGGCCCAA CCCTGTGTTT
    CTTGTCTATG ACTTCATGCT GCTCCTGTGT GCTTCCTTAC AACGACAGAT TTTTGAGGAC
    GAAAACAAGG CAGCTGTGCG CATCATGGCC GGGGACAATG TCGAGATTTG CATGAACCTT
    GACGCGGCCT CATTCAGCCA GCATAACCCT GTACCAGATT TCATTCACTG CAGATCTTAC
    TTGGACATGT CCAAAGTGAT CATCTTCAGC TACCTCTTCT GGTTCGTCCT CACCATCATC
    TTCATTACCG GAACGACCAG GATCAGCATC TTTTGCATGG GTTACCTGGT GGCCTGTTTC
    TATTTCCTGC TCTTTGGAGG TGATCTGCTC CTGAAACCCA TCAAGAGCAT CCTACGCTAC
    TGGGACTGGC TGATTGCATA CAACGTGTTT GTGATCACCA TGAAAAACAT ACTGTCAATA
    GGAGCCTGTG GATACATCGG TGCACTGGTG AGAAACAGCT GCTGGCTGAT CCAAGCGTTC
    AGCCTGGCCT GCACGGTGAA AGGCTATAAA ATGCCTGAAG ATGACTCCAG ATGCAAGCTG
    CCCAGTGGGG AGGCAGGGAT CATCTGGGAC AGCATCTGCT TTGCGTTTCT CCTGCTACAG
    AGGAGAGTCT TCATGAGTTA CTATTTCCTG CATGTCGTGG CTGACATTAA AGCATCCCAG
    ATCCTGGCAT CCAGAGGGGC CGAACTTTTC CAGGCTACAA TTGTGAAAGC TGTAAAGGCA
    AGAATTGAAG AAGAGAAAAA ATCCATGGAC CAGCTGAAGA GACAGATGGA TCGCATCAAA
    GCCCGGCAGC AAAAGTACAA AAAGGGTAAG GAGAGGATGC TGAGCTTGAC CCAGGAGTCA
    GGGGAAGGCC AGGACATCCA GAAAGTCTCA GAAGAAGACG ATGAAAGAGA AGCAGACAAA
    CAGAAAGCCA AGGGCAAAAA AAAGCAGTGG TGGCGGCCTT GGGTTGATCA TGCTTCCATG
    GTCAGGAGTG GAGATTATTA TTTGTTTGAA ACGGATAGTG AAGAGGAGGA AGAAGAGGAA
    CTCAAAAAGG AGGATGAAGA ACCACCACGG AAGTCTGCAT TCCAGTTCGT CTACCAAGCC
    TGGATTACCG ATCCTAAAAC AGCACTCCGA CAGAGGAGGA AAGAGAAGAA GAAAATGGCC
    AGAGAAGAGC AGAAGGGAAG ACGCAAAGGA TCTGGGGAGG GTCCAGTGGA ATGGGAAGAC
    AGAGAAGATG AGCCAATCAA AAAGAAATCT GATGGACCAG ATAATATCAT CAAGAGGATA
    TTTAATATCC TGAAGTTCAC CTGGGTCCTG TTCCTGGCCA CAGTGGACAG TTTCACTACT
    TGGCTCAACT CCATCTCAAG AGAGCATATC GACATATCCA CAGTTCTGCG GATTGAAAGA
    TGCATGCTGA CCCGAGAGAT AAAGAAGGGC AACGTTCCGA CACGGGAAAG CATCCACATG
    TACTACCAGA ACCACATGAT GAACCTTTCC AGAGAATCCG GACTGGACAC AATCGACGAG
    CACTCTGGCG CTGGTTCCAG GGCCCAGGCA GCACACAGGA TGGATAGCTT AGATTCCCAT
    GACAGTATCT CCAGCTGTTA CACTGAAGCA ACCCTGCTGA TCTCAAGGCA GTCCACCCTT
    GATGATTTAG ATGGACAAGA CCCTGTTCCT AAAACAAGCG AGCGTGCTCG GCCTAGGCTC
    CGTAAAGTGT TCAGCATGGA CATGTCCTCC TCATCAGCTG ACAGTGGCAG TGTAGCATCA
    AGCGAGCCCA CACAGTGCAC CATGCTGTAT TCACGCCAAG GAACTACGGA AACCATTGAG
    GAGGTAGAGG CTGAAGCTGA GGAGGAGGTG GTAGAGAGTC TGGAGCCTGA GCTCCATGAT
    GCCGAAGAGA AGGAATATGC AGCTGACTAT GAAGCAGGGG TAGAGGAGGT CAGCCTCACT
    CCAGATGAGG AGTTGCCTCA GTTCTCAACA GATGACGGTG ACGTGGAGGC CCCGCCCTCC
    TACAGCAAGG CTGTCAGCTT TGAGCACCTC TCGTTTGCAT CACAAGATGA CTCCGGGGGC
    AAGAACCACA TGGTGGTCAG CCCTGACGAC AGCCGCACAG ACAAGCTGGA GTCCAGTATC
    TTACCGCCCC TGACTCACGA GCTGACAGCC AGCGATCTGC TCATGAGCAA GATGTTCCGT
    GATGATGAGC TTGAAGAATC TGAGAAATTC TACGTGGACC AGCCCCGGTT CTTGCTGCTA
    TTCTATGCCA TGTATAACAC TCTGGTAGCC CGCTCGGAAA TGGTGTGCTA TTTCGTGATC
    ATCCTGAACC ACATGATCTC TGCCTCCATC ATTACTCTTC TGTTGCCCAT CCTGATCTTC
    CTCTGGGCCA TGCTGTCCGT CCCCAGGCCC AGCCGGCGCT TCTGGATGAT GGCCATCGTC
    TACACTGAGG TGGCGATTGT AGTGAAATAT TTCTTCCAGT TTGGCTTCTT TCCTTGGAAT
    AAGGACTTGG AAATTTACAA GGACAGGCCC TACTTTCCAC CAAACATCAT AGGGGTGGAA
    AAGAAAGAGG GCTATGTTCT TTACGACCTC ATTCAGCTGC TGGCTCTGTT CTTCCATCGA
    TCCATTCTGA AGTGTCATGG CCTATGGGAC GAGGATGACA TTGTTGACAG TAACACAGAC
    AAGGAAGGAT CTGATGATGA GCTGTCCCTC GGTCAGGGCA GAAGAGGCTC CTCAGAGTCA
    CTGAAGTCCA TCAACTTGGC TGCCTCTGTA GAGTCCGTCC ATGTGACCTT CCCAGAGCAG
    CCAGCAGCCA TCCGGAGAAA ACGCTCCTGC AGCAGCTCGC AGATCTCCCC ACGGTCCAGC
    TTCTCTTCAA ATAGGTCCAA AAGAGGCAGC ACAAGCACCC GAAACAGCAG CCAAAAAGGA
    AGCAGTGTTT TGAGCATTAA ACAAAAAAGC AAAAGAGAAC TTTACATGGA AAAGCTTCAA
    GAACATTTAA TCAAAGCAAA GGCCTTTACC ATAAAGAAAA CTCTACAAAT CTATGTACCC
    ATTCGGCAAT TCTTCTATGA CCTCATCCAC CCAGACTACA GTGCTGTGAC TGACGTGTAT
    GTGCTGATGT TCCTGGCCGA CACCGTGGAC TTCATCATCA TCGTCTTTGG CTTCTGGGCT
    TTTGGGAAAC ACTCGGCAGC TGCAGATATC ACCTCTTCCC TATCAGAAGA CCAGGTCCCC
    GGGCCATTCC TGGTGATGGT TCTCATTCAG TTTGGAACCA TGGTGGTGGA CCGTGCACTC
    TACCTCAGGA AGACTGTGCT TGGCAAGGTC ATTTTCCAGG TCATCCTCGT GTTTGGAATT
    CACTTCTGGA TGTTCTTCAT CCTACCCAGT GTGACTGAGA GGAAATTCAG CCAGAACCTG
    GTGGCACAGC TCTGGTACTT TGTGAAATGT GTGTACTTTG GGTTGTCTGC CTACCAAATC
    CGCTGTGGCT ACCCAACACG TGTGCTCGGA AACTTCCTCA CGAAGAGCTA CAATTATGTC
    AACCTCTTCT TGTTTCAAGG GTTTCGTCTC GTCCCATTTC TGACAGAGCT GCGGGCTGTG
    ATGGACTGGG TGTGGACAGA TACAACCTTG AGCCTCTCCA GCTGGATTTG TGTGGAGGAC
    ATTTACGCAC ACATTTTCAT CTTAAAGTGC TGGCGAGAGT CGGAAAAGAG ATACCCTCAA
    CCCAGGGGCC AGAAGAAGAA GAAAGCTGTG AAGTATGGCA TGGGCGGAAT GATCATTGTC
    CTGCTCATCT GTATCGTCTG GTTTCCTCTG CTCTTCATGT CTTTGATCAA GTCTGTCGCA
    GGGGTTATTA ACCAACCCCT GGATGTGTCC GTCACAATAA CCCTGGGAGG CTATCAGCCT
    ATCTTCACAA TGAGTGCCCA GCAAAGCCAG CTGAAAGTTA TGGATGGTAC AAAGTATAAC
    GAATTCATAA AGTCTTTCGG CCCCAACAGT GGCGCTATGC AATTTCTGGA GAATTATGAA
    AGGCAAGACA TAACAGTAGC GGAACTGGAA GGGAATTCGA ATTCTTTGTG GACCATCAGC
    CCTCCCAGTA AGCAGAAAAT GATACAGGAG CTCACCGACC CCAATAGTTG CTTCTCTGTT
    GTTTTTTCAT GGAGTATCCA GAGAAACATG ACTCTGGGTG CAAAGGCTGA AATAGCAACG
    GACAAGCTTT CTTTTCCTCT CACGGATGCG ACACGAAGTA GCATAGCCAA AATGATTGCT
    GGCAATGACA CAGAAAGTTC CAACACACCA GTGACAATAG AAAAGATCTA CCCATACTAT
    GTGAAGGCAC CCAGTGACTC AAACTCAAAG CCTATAAAAC AGCTTCTGTC AGAAAACAAT
    TTCATGAATA TCACCATCAT TTTGTTCAGA GACAATGTCA CAAAGTCCAA TAGTGAGTGG
    TGGGTTCTCA ACCTGACTGG AAGTAGGATA TTCAATCAGG GATCCCAAGC CTTGGAGCTG
    GTGGTCTTCA ATGACAAAGT CAGCCCCCCA AGTCTGGGCT TCTTGGCTGG CTATGGCATC
    ATGGGATTGT ATGCATCCGT TGTCCTTGTA ATTGGGAAGT TTGTTCGCGA ATTCTTCAGT
    GGGATTTCTC ACTCTATCAT GTTTGAAGAG CTTCCAAATG TGGACAGAAT TTTGAAGTTA
    TGCACAGATA TATTTCTCGT ACGGGAGACA GGGGAACTGG AGCTGGAGGA AGACCTCTAT
    GCCAAATTGA TATTCCTGTA CAGGTCGCCA GAAACCATGA TCAAGTGGAC TAGAGAAAAA
    ACAAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa20913
    Clone ID Related Accession (Same CDS sequence) XM_006254839.3 , XM_006222595.3
    Accession Version XM_006222595.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8469bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product piezo-type mechanosensitive ion channel component 2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000086.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006222595.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTCCCCAT CTGCCTGGCA 
    GTAGCATGTG CATTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCG ACGATGCAAG GACACACAGG GCGGCTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCCTTT CTGCTCCTTC ACATCATTTT CCACATCACA
    CTGGCCAGCC TGGAAGCTCA GCACCGCATC GCACCCGCTT ACAACTGCTC AACTTGGGAA
    AAGACCTTCC GGCAGATCGG CTTCGAAAGC TTGAAGGGAG CTGATGCTGG CAATGGCATC
    AGGGTGTTTG TGCCTGATAT CGGGATGTTC ATTGCGAGCC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACGGAA GAAGTAGCCC AATTTAATTC CGAGTGCGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GACTCAGAAG AAGCGCTGAT TTATGAGGAG
    GACTTAGATG GGGAAGAAGG CATGGAAGGC GAGCTGGAAG AAAGCACAAA GTTAAAAATG
    TTTCGCAGGC TCGCCTCCGT CGCTTCCAAG CTTAAGGAGT TCATCGGCAA TATGATAACC
    ACGGCGGGCA AGGTGGTGGT GACCATCCTG TTGGGCTCCT CTGGCATGAT GCTGCCATCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT CTGGGTCTGT GCACCTGGTG GTCCTGGTGC
    CGAACTTTTG ACCCATTGCT GTTTGGCTGC CTCTGTGTCC TGTTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTTCAGTTCT TCCAAGAAGC AGTCCCACCC
    AATGACTACT ATGCAAGGTT GTTTGGAATC AAATCCGTTA TTCAAACAGA CTGCTCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTACAC GCTGGCCACG CTGATCCGCA TTTGGTTGCA AGAGCCTCTT
    GTGCAGGAGG AGATGTCGAA GGAAGAGGAG GGTGCACTGG ATGGCTGCTC CAATCAGAAC
    ACAGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    CTTTTATCCA TGACCCAAGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACTGTGAAC
    GGCAACCCTG TGGACTACCA CACCATCCAC CCAAGTCTGC CCATAGAGAA TGGCCCCGCC
    AAGACGGACT TGTATACTAC TCCTCAGTAC CGGTGGGAGC CCTCAGAGGA GTCCTCAGAA
    AAGAAAGAGG AGGAAGAGGA CAAGAGAGAA GACTCAGAAG GGGAAGGGAG CCAAGAGGAA
    AAACGGAGTG TGAGAATTCA TGCCATGGTC GCCGTGTTCC AGTTCATCAT GAAGCAGAGT
    TACATCTGTG CCCTCATCGC CATGATGGCA TGGAGCATCA CCTACCACAG CTGGCTGACA
    TTTGTGCTGT TGATCTGGTC GTGCACTCTC TGGATGATTC GCAACAGGAG GAAGTACGCC
    ATGATCAGCT CTCCGTTCAT GGTTGTCTAT GCCAATCTGC TGCTGGTACT GCAGTACATA
    TGGAGCTTTG AACTTCCAGA AATCAAGAAA GTCCCTGGAT TTCTAGAAAA GAAAGAGCCT
    GGAGAGCTTG CCTCAAAGAT CCTCTTCACC ATCACATTTT GGCTGCTCCT GAGGCAGCAT
    CTCACAGAGC AGAAGGCCCT TCAGGAAAAG GAAGCACTTC TGTCTGAAGT CAAAATTGGA
    AGTCAAGAAC TTGACGAAAA GGATGAGGAA CTCCAAGATA TACAAGTGGA AGGAGAGCCC
    ATAGAGAAGG AAGAAGAGGA GGAGGAAATA AAGGAAGACA GACATGAGGT AAAGAAGGAA
    GAGGAAGAGG AAGAGGAAGA TGACGATGAC CAGGACATCA TGAAGGTTCT GGGCAATCTG
    GTGGTGGCCC TCTTCATCAA GTATTGGATC TACGTCTGCG GGGGCATGTT CTTTTTTGTC
    AGCTTTGAGG GGAAAATCGT CATGTACAAA ATCATCTACA TGGTGCTGTT CCTGTTCTGT
    GTGGCCTTGT ACCAGGTGCA CTACGAGTGG TGGAGGAAGA TCCTGAAATA CTTCTGGATG
    TCGGTGGTCA TTTACACCAT GCTGGTGCTC ATCTTCATCT ACACTTACCA GTTTGAGAAC
    TTCCCAGGCC TGTGGCAAAA CATGACCGGG TTAAAGAAAG AGAAGCTTGA GGATCTGGGC
    CTGAAGCAGT TCACTGTGGC CGAACTGTTC ACACGAATAT TCATCCCCAC CTCCTTTCTG
    CTGGTCTGCA TCCTGCACCT CCACTATTTC CATGACCGGT TCCTGGAACT CACAGACCTC
    AAGTCCATCC CCAGCAAAGA AGACAACACC ATCTACAGCC ATGCCAAGGT CAATGGTCGC
    GTGTACCTAA TAATAAATAG GTTAGCCCAC CCTGAAGGAA GCCTCCCAGA CCTAGCCATT
    ATGAACATGA CGGCCAGCCT CGATAAGCCT GAGAGGCAGA AGTTGGCAGA GCCTGGGGAG
    GAGAGGACAG AAGAATGTGC CAAGAAGGCA GAGAAGGGTG AGGCTGGGAA AGACAGTGAT
    GAGTCTGAGG AGGAGGAAGG TGAGGAAGAG GAATCCGAGG AGGAAGAATC CTCAGATCTC
    AGGAACAAGT GGCACCTCGT GATTGACCGG CTCACTGTGC TCTTTCTAAA GTTCCTGGAG
    TATTTCCACA AGTTGCAGGT GTTTATGTGG TGGATCTTGG AGCTGCACAT CATCAAAATC
    GTCTCATCGT ACATTATCTG GGTGACTGTC AAAGAGGTGT CTCTGTTCAA CTATGTATTT
    TTGATTTCTT GGGCATTTGC TCTGCCATAT GCCAAACTCC GCCGTGTGGC TTCCAGTGTC
    TGCACTGTCT GGACGTGTGT GATCATCGTC TGCAAAATGT TGTACCAGCT GCAAACCATT
    AAGCCTGAGA ACTTTTCTGT CAACTGCTCC TTGCCCAATG AAAATCAAAC CAACATACCC
    CTTAATGAGT TGAACAAGTC TCTTCTCTAC AGTGCCCCTG TTGATCCCAC TGAGTGGGTT
    GGCCTGAGGA AGTCCTCCCC TTTACTCGTC TACCTGAGGA ACAACCTCCT GATGTTGGCG
    ATTTTGGCCT TCGAAGTCAC TGTTTACCGA CATCAGGAGT ACTATCGAGG CCGAAACAAC
    TTGACTGCTC CGGTGTCTAA AACTATCTTT CATGACATCA CAAGGTTACA TCTAGATGAT
    GGGCTTATCA ATTGTGCCAA ATATTTTGTA AACTACTTCT TCTACAAGTT TGGCCTAGAG
    ACCTGTTTCC TAATGTCAGT GAACGTAATT GGACAGCGAA TGGACTTCTA CGCCATGATT
    CATGCCTGTT GGCTGATCGG TGTCTTGTAC CGTCGCAGAA GAAAAGCCAT TGCTGAGGTC
    TGGCCCAAGT ACTGCTGCTT CCTGGCTTGC ATCATCACCT TCCAGTACTT TGTCTGCATA
    GGCATCCCTC CTGCCCCCTG CCGAGATTAC CCATGGAGGT TCAAGGGTGC CAACTTCAAT
    GACAACATCA TCAAGTGGCT GTACTTCCCA GATTTCATCG TGCGGCCCAA CCCTGTGTTT
    CTTGTCTATG ACTTCATGCT GCTCCTGTGT GCTTCCTTAC AACGACAGAT TTTTGAGGAC
    GAAAACAAGG CAGCTGTGCG CATCATGGCC GGGGACAATG TCGAGATTTG CATGAACCTT
    GACGCGGCCT CATTCAGCCA GCATAACCCT GTACCAGATT TCATTCACTG CAGATCTTAC
    TTGGACATGT CCAAAGTGAT CATCTTCAGC TACCTCTTCT GGTTCGTCCT CACCATCATC
    TTCATTACCG GAACGACCAG GATCAGCATC TTTTGCATGG GTTACCTGGT GGCCTGTTTC
    TATTTCCTGC TCTTTGGAGG TGATCTGCTC CTGAAACCCA TCAAGAGCAT CCTACGCTAC
    TGGGACTGGC TGATTGCATA CAACGTGTTT GTGATCACCA TGAAAAACAT ACTGTCAATA
    GGAGCCTGTG GATACATCGG TGCACTGGTG AGAAACAGCT GCTGGCTGAT CCAAGCGTTC
    AGCCTGGCCT GCACGGTGAA AGGCTATAAA ATGCCTGAAG ATGACTCCAG ATGCAAGCTG
    CCCAGTGGGG AGGCAGGGAT CATCTGGGAC AGCATCTGCT TTGCGTTTCT CCTGCTACAG
    AGGAGAGTCT TCATGAGTTA CTATTTCCTG CATGTCGTGG CTGACATTAA AGCATCCCAG
    ATCCTGGCAT CCAGAGGGGC CGAACTTTTC CAGGCTACAA TTGTGAAAGC TGTAAAGGCA
    AGAATTGAAG AAGAGAAAAA ATCCATGGAC CAGCTGAAGA GACAGATGGA TCGCATCAAA
    GCCCGGCAGC AAAAGTACAA AAAGGGTAAG GAGAGGATGC TGAGCTTGAC CCAGGAGTCA
    GGGGAAGGCC AGGACATCCA GAAAGTCTCA GAAGAAGACG ATGAAAGAGA AGCAGACAAA
    CAGAAAGCCA AGGGCAAAAA AAAGCAGTGG TGGCGGCCTT GGGTTGATCA TGCTTCCATG
    GTCAGGAGTG GAGATTATTA TTTGTTTGAA ACGGATAGTG AAGAGGAGGA AGAAGAGGAA
    CTCAAAAAGG AGGATGAAGA ACCACCACGG AAGTCTGCAT TCCAGTTCGT CTACCAAGCC
    TGGATTACCG ATCCTAAAAC AGCACTCCGA CAGAGGAGGA AAGAGAAGAA GAAAATGGCC
    AGAGAAGAGC AGAAGGGAAG ACGCAAAGGA TCTGGGGAGG GTCCAGTGGA ATGGGAAGAC
    AGAGAAGATG AGCCAATCAA AAAGAAATCT GATGGACCAG ATAATATCAT CAAGAGGATA
    TTTAATATCC TGAAGTTCAC CTGGGTCCTG TTCCTGGCCA CAGTGGACAG TTTCACTACT
    TGGCTCAACT CCATCTCAAG AGAGCATATC GACATATCCA CAGTTCTGCG GATTGAAAGA
    TGCATGCTGA CCCGAGAGAT AAAGAAGGGC AACGTTCCGA CACGGGAAAG CATCCACATG
    TACTACCAGA ACCACATGAT GAACCTTTCC AGAGAATCCG GACTGGACAC AATCGACGAG
    CACTCTGGCG CTGGTTCCAG GGCCCAGGCA GCACACAGGA TGGATAGCTT AGATTCCCAT
    GACAGTATCT CCAGCTGTTA CACTGAAGCA ACCCTGCTGA TCTCAAGGCA GTCCACCCTT
    GATGATTTAG ATGGACAAGA CCCTGTTCCT AAAACAAGCG AGCGTGCTCG GCCTAGGCTC
    CGTAAAGTGT TCAGCATGGA CATGTCCTCC TCATCAGCTG ACAGTGGCAG TGTAGCATCA
    AGCGAGCCCA CACAGTGCAC CATGCTGTAT TCACGCCAAG GAACTACGGA AACCATTGAG
    GAGGTAGAGG CTGAAGCTGA GGAGGAGGTG GTAGAGAGTC TGGAGCCTGA GCTCCATGAT
    GCCGAAGAGA AGGAATATGC AGCTGACTAT GAAGCAGGGG TAGAGGAGGT CAGCCTCACT
    CCAGATGAGG AGTTGCCTCA GTTCTCAACA GATGACGGTG ACGTGGAGGC CCCGCCCTCC
    TACAGCAAGG CTGTCAGCTT TGAGCACCTC TCGTTTGCAT CACAAGATGA CTCCGGGGGC
    AAGAACCACA TGGTGGTCAG CCCTGACGAC AGCCGCACAG ACAAGCTGGA GTCCAGTATC
    TTACCGCCCC TGACTCACGA GCTGACAGCC AGCGATCTGC TCATGAGCAA GATGTTCCGT
    GATGATGAGC TTGAAGAATC TGAGAAATTC TACGTGGACC AGCCCCGGTT CTTGCTGCTA
    TTCTATGCCA TGTATAACAC TCTGGTAGCC CGCTCGGAAA TGGTGTGCTA TTTCGTGATC
    ATCCTGAACC ACATGATCTC TGCCTCCATC ATTACTCTTC TGTTGCCCAT CCTGATCTTC
    CTCTGGGCCA TGCTGTCCGT CCCCAGGCCC AGCCGGCGCT TCTGGATGAT GGCCATCGTC
    TACACTGAGG TGGCGATTGT AGTGAAATAT TTCTTCCAGT TTGGCTTCTT TCCTTGGAAT
    AAGGACTTGG AAATTTACAA GGACAGGCCC TACTTTCCAC CAAACATCAT AGGGGTGGAA
    AAGAAAGAGG GCTATGTTCT TTACGACCTC ATTCAGCTGC TGGCTCTGTT CTTCCATCGA
    TCCATTCTGA AGTGTCATGG CCTATGGGAC GAGGATGACA TTGTTGACAG TAACACAGAC
    AAGGAAGGAT CTGATGATGA GCTGTCCCTC GGTCAGGGCA GAAGAGGCTC CTCAGAGTCA
    CTGAAGTCCA TCAACTTGGC TGCCTCTGTA GAGTCCGTCC ATGTGACCTT CCCAGAGCAG
    CCAGCAGCCA TCCGGAGAAA ACGCTCCTGC AGCAGCTCGC AGATCTCCCC ACGGTCCAGC
    TTCTCTTCAA ATAGGTCCAA AAGAGGCAGC ACAAGCACCC GAAACAGCAG CCAAAAAGGA
    AGCAGTGTTT TGAGCATTAA ACAAAAAAGC AAAAGAGAAC TTTACATGGA AAAGCTTCAA
    GAACATTTAA TCAAAGCAAA GGCCTTTACC ATAAAGAAAA CTCTACAAAT CTATGTACCC
    ATTCGGCAAT TCTTCTATGA CCTCATCCAC CCAGACTACA GTGCTGTGAC TGACGTGTAT
    GTGCTGATGT TCCTGGCCGA CACCGTGGAC TTCATCATCA TCGTCTTTGG CTTCTGGGCT
    TTTGGGAAAC ACTCGGCAGC TGCAGATATC ACCTCTTCCC TATCAGAAGA CCAGGTCCCC
    GGGCCATTCC TGGTGATGGT TCTCATTCAG TTTGGAACCA TGGTGGTGGA CCGTGCACTC
    TACCTCAGGA AGACTGTGCT TGGCAAGGTC ATTTTCCAGG TCATCCTCGT GTTTGGAATT
    CACTTCTGGA TGTTCTTCAT CCTACCCAGT GTGACTGAGA GGAAATTCAG CCAGAACCTG
    GTGGCACAGC TCTGGTACTT TGTGAAATGT GTGTACTTTG GGTTGTCTGC CTACCAAATC
    CGCTGTGGCT ACCCAACACG TGTGCTCGGA AACTTCCTCA CGAAGAGCTA CAATTATGTC
    AACCTCTTCT TGTTTCAAGG GTTTCGTCTC GTCCCATTTC TGACAGAGCT GCGGGCTGTG
    ATGGACTGGG TGTGGACAGA TACAACCTTG AGCCTCTCCA GCTGGATTTG TGTGGAGGAC
    ATTTACGCAC ACATTTTCAT CTTAAAGTGC TGGCGAGAGT CGGAAAAGAG ATACCCTCAA
    CCCAGGGGCC AGAAGAAGAA GAAAGCTGTG AAGTATGGCA TGGGCGGAAT GATCATTGTC
    CTGCTCATCT GTATCGTCTG GTTTCCTCTG CTCTTCATGT CTTTGATCAA GTCTGTCGCA
    GGGGTTATTA ACCAACCCCT GGATGTGTCC GTCACAATAA CCCTGGGAGG CTATCAGCCT
    ATCTTCACAA TGAGTGCCCA GCAAAGCCAG CTGAAAGTTA TGGATGGTAC AAAGTATAAC
    GAATTCATAA AGTCTTTCGG CCCCAACAGT GGCGCTATGC AATTTCTGGA GAATTATGAA
    AGGCAAGACA TAACAGTAGC GGAACTGGAA GGGAATTCGA ATTCTTTGTG GACCATCAGC
    CCTCCCAGTA AGCAGAAAAT GATACAGGAG CTCACCGACC CCAATAGTTG CTTCTCTGTT
    GTTTTTTCAT GGAGTATCCA GAGAAACATG ACTCTGGGTG CAAAGGCTGA AATAGCAACG
    GACAAGCTTT CTTTTCCTCT CACGGATGCG ACACGAAGTA GCATAGCCAA AATGATTGCT
    GGCAATGACA CAGAAAGTTC CAACACACCA GTGACAATAG AAAAGATCTA CCCATACTAT
    GTGAAGGCAC CCAGTGACTC AAACTCAAAG CCTATAAAAC AGCTTCTGTC AGAAAACAAT
    TTCATGAATA TCACCATCAT TTTGTTCAGA GACAATGTCA CAAAGTCCAA TAGTGAGTGG
    TGGGTTCTCA ACCTGACTGG AAGTAGGATA TTCAATCAGG GATCCCAAGC CTTGGAGCTG
    GTGGTCTTCA ATGACAAAGT CAGCCCCCCA AGTCTGGGCT TCTTGGCTGG CTATGGCATC
    ATGGGATTGT ATGCATCCGT TGTCCTTGTA ATTGGGAAGT TTGTTCGCGA ATTCTTCAGT
    GGGATTTCTC ACTCTATCAT GTTTGAAGAG CTTCCAAATG TGGACAGAAT TTTGAAGTTA
    TGCACAGATA TATTTCTCGT ACGGGAGACA GGGGAACTGG AGCTGGAGGA AGACCTCTAT
    GCCAAATTGA TATTCCTGTA CAGGTCGCCA GAAACCATGA TCAAGTGGAC TAGAGAAAAA
    ACAAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006222657.1
    CDS940..9408
    Translation

    Target ORF information:

    RefSeq Version XM_006222595.3
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006222595.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTCCCCAT CTGCCTGGCA 
    GTAGCATGTG CATTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCG ACGATGCAAG GACACACAGG GCGGCTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCCTTT CTGCTCCTTC ACATCATTTT CCACATCACA
    CTGGCCAGCC TGGAAGCTCA GCACCGCATC GCACCCGCTT ACAACTGCTC AACTTGGGAA
    AAGACCTTCC GGCAGATCGG CTTCGAAAGC TTGAAGGGAG CTGATGCTGG CAATGGCATC
    AGGGTGTTTG TGCCTGATAT CGGGATGTTC ATTGCGAGCC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACGGAA GAAGTAGCCC AATTTAATTC CGAGTGCGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GACTCAGAAG AAGCGCTGAT TTATGAGGAG
    GACTTAGATG GGGAAGAAGG CATGGAAGGC GAGCTGGAAG AAAGCACAAA GTTAAAAATG
    TTTCGCAGGC TCGCCTCCGT CGCTTCCAAG CTTAAGGAGT TCATCGGCAA TATGATAACC
    ACGGCGGGCA AGGTGGTGGT GACCATCCTG TTGGGCTCCT CTGGCATGAT GCTGCCATCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT CTGGGTCTGT GCACCTGGTG GTCCTGGTGC
    CGAACTTTTG ACCCATTGCT GTTTGGCTGC CTCTGTGTCC TGTTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTTCAGTTCT TCCAAGAAGC AGTCCCACCC
    AATGACTACT ATGCAAGGTT GTTTGGAATC AAATCCGTTA TTCAAACAGA CTGCTCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTACAC GCTGGCCACG CTGATCCGCA TTTGGTTGCA AGAGCCTCTT
    GTGCAGGAGG AGATGTCGAA GGAAGAGGAG GGTGCACTGG ATGGCTGCTC CAATCAGAAC
    ACAGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    CTTTTATCCA TGACCCAAGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACTGTGAAC
    GGCAACCCTG TGGACTACCA CACCATCCAC CCAAGTCTGC CCATAGAGAA TGGCCCCGCC
    AAGACGGACT TGTATACTAC TCCTCAGTAC CGGTGGGAGC CCTCAGAGGA GTCCTCAGAA
    AAGAAAGAGG AGGAAGAGGA CAAGAGAGAA GACTCAGAAG GGGAAGGGAG CCAAGAGGAA
    AAACGGAGTG TGAGAATTCA TGCCATGGTC GCCGTGTTCC AGTTCATCAT GAAGCAGAGT
    TACATCTGTG CCCTCATCGC CATGATGGCA TGGAGCATCA CCTACCACAG CTGGCTGACA
    TTTGTGCTGT TGATCTGGTC GTGCACTCTC TGGATGATTC GCAACAGGAG GAAGTACGCC
    ATGATCAGCT CTCCGTTCAT GGTTGTCTAT GCCAATCTGC TGCTGGTACT GCAGTACATA
    TGGAGCTTTG AACTTCCAGA AATCAAGAAA GTCCCTGGAT TTCTAGAAAA GAAAGAGCCT
    GGAGAGCTTG CCTCAAAGAT CCTCTTCACC ATCACATTTT GGCTGCTCCT GAGGCAGCAT
    CTCACAGAGC AGAAGGCCCT TCAGGAAAAG GAAGCACTTC TGTCTGAAGT CAAAATTGGA
    AGTCAAGAAC TTGACGAAAA GGATGAGGAA CTCCAAGATA TACAAGTGGA AGGAGAGCCC
    ATAGAGAAGG AAGAAGAGGA GGAGGAAATA AAGGAAGACA GACATGAGGT AAAGAAGGAA
    GAGGAAGAGG AAGAGGAAGA TGACGATGAC CAGGACATCA TGAAGGTTCT GGGCAATCTG
    GTGGTGGCCC TCTTCATCAA GTATTGGATC TACGTCTGCG GGGGCATGTT CTTTTTTGTC
    AGCTTTGAGG GGAAAATCGT CATGTACAAA ATCATCTACA TGGTGCTGTT CCTGTTCTGT
    GTGGCCTTGT ACCAGGTGCA CTACGAGTGG TGGAGGAAGA TCCTGAAATA CTTCTGGATG
    TCGGTGGTCA TTTACACCAT GCTGGTGCTC ATCTTCATCT ACACTTACCA GTTTGAGAAC
    TTCCCAGGCC TGTGGCAAAA CATGACCGGG TTAAAGAAAG AGAAGCTTGA GGATCTGGGC
    CTGAAGCAGT TCACTGTGGC CGAACTGTTC ACACGAATAT TCATCCCCAC CTCCTTTCTG
    CTGGTCTGCA TCCTGCACCT CCACTATTTC CATGACCGGT TCCTGGAACT CACAGACCTC
    AAGTCCATCC CCAGCAAAGA AGACAACACC ATCTACAGCC ATGCCAAGGT CAATGGTCGC
    GTGTACCTAA TAATAAATAG GTTAGCCCAC CCTGAAGGAA GCCTCCCAGA CCTAGCCATT
    ATGAACATGA CGGCCAGCCT CGATAAGCCT GAGAGGCAGA AGTTGGCAGA GCCTGGGGAG
    GAGAGGACAG AAGAATGTGC CAAGAAGGCA GAGAAGGGTG AGGCTGGGAA AGACAGTGAT
    GAGTCTGAGG AGGAGGAAGG TGAGGAAGAG GAATCCGAGG AGGAAGAATC CTCAGATCTC
    AGGAACAAGT GGCACCTCGT GATTGACCGG CTCACTGTGC TCTTTCTAAA GTTCCTGGAG
    TATTTCCACA AGTTGCAGGT GTTTATGTGG TGGATCTTGG AGCTGCACAT CATCAAAATC
    GTCTCATCGT ACATTATCTG GGTGACTGTC AAAGAGGTGT CTCTGTTCAA CTATGTATTT
    TTGATTTCTT GGGCATTTGC TCTGCCATAT GCCAAACTCC GCCGTGTGGC TTCCAGTGTC
    TGCACTGTCT GGACGTGTGT GATCATCGTC TGCAAAATGT TGTACCAGCT GCAAACCATT
    AAGCCTGAGA ACTTTTCTGT CAACTGCTCC TTGCCCAATG AAAATCAAAC CAACATACCC
    CTTAATGAGT TGAACAAGTC TCTTCTCTAC AGTGCCCCTG TTGATCCCAC TGAGTGGGTT
    GGCCTGAGGA AGTCCTCCCC TTTACTCGTC TACCTGAGGA ACAACCTCCT GATGTTGGCG
    ATTTTGGCCT TCGAAGTCAC TGTTTACCGA CATCAGGAGT ACTATCGAGG CCGAAACAAC
    TTGACTGCTC CGGTGTCTAA AACTATCTTT CATGACATCA CAAGGTTACA TCTAGATGAT
    GGGCTTATCA ATTGTGCCAA ATATTTTGTA AACTACTTCT TCTACAAGTT TGGCCTAGAG
    ACCTGTTTCC TAATGTCAGT GAACGTAATT GGACAGCGAA TGGACTTCTA CGCCATGATT
    CATGCCTGTT GGCTGATCGG TGTCTTGTAC CGTCGCAGAA GAAAAGCCAT TGCTGAGGTC
    TGGCCCAAGT ACTGCTGCTT CCTGGCTTGC ATCATCACCT TCCAGTACTT TGTCTGCATA
    GGCATCCCTC CTGCCCCCTG CCGAGATTAC CCATGGAGGT TCAAGGGTGC CAACTTCAAT
    GACAACATCA TCAAGTGGCT GTACTTCCCA GATTTCATCG TGCGGCCCAA CCCTGTGTTT
    CTTGTCTATG ACTTCATGCT GCTCCTGTGT GCTTCCTTAC AACGACAGAT TTTTGAGGAC
    GAAAACAAGG CAGCTGTGCG CATCATGGCC GGGGACAATG TCGAGATTTG CATGAACCTT
    GACGCGGCCT CATTCAGCCA GCATAACCCT GTACCAGATT TCATTCACTG CAGATCTTAC
    TTGGACATGT CCAAAGTGAT CATCTTCAGC TACCTCTTCT GGTTCGTCCT CACCATCATC
    TTCATTACCG GAACGACCAG GATCAGCATC TTTTGCATGG GTTACCTGGT GGCCTGTTTC
    TATTTCCTGC TCTTTGGAGG TGATCTGCTC CTGAAACCCA TCAAGAGCAT CCTACGCTAC
    TGGGACTGGC TGATTGCATA CAACGTGTTT GTGATCACCA TGAAAAACAT ACTGTCAATA
    GGAGCCTGTG GATACATCGG TGCACTGGTG AGAAACAGCT GCTGGCTGAT CCAAGCGTTC
    AGCCTGGCCT GCACGGTGAA AGGCTATAAA ATGCCTGAAG ATGACTCCAG ATGCAAGCTG
    CCCAGTGGGG AGGCAGGGAT CATCTGGGAC AGCATCTGCT TTGCGTTTCT CCTGCTACAG
    AGGAGAGTCT TCATGAGTTA CTATTTCCTG CATGTCGTGG CTGACATTAA AGCATCCCAG
    ATCCTGGCAT CCAGAGGGGC CGAACTTTTC CAGGCTACAA TTGTGAAAGC TGTAAAGGCA
    AGAATTGAAG AAGAGAAAAA ATCCATGGAC CAGCTGAAGA GACAGATGGA TCGCATCAAA
    GCCCGGCAGC AAAAGTACAA AAAGGGTAAG GAGAGGATGC TGAGCTTGAC CCAGGAGTCA
    GGGGAAGGCC AGGACATCCA GAAAGTCTCA GAAGAAGACG ATGAAAGAGA AGCAGACAAA
    CAGAAAGCCA AGGGCAAAAA AAAGCAGTGG TGGCGGCCTT GGGTTGATCA TGCTTCCATG
    GTCAGGAGTG GAGATTATTA TTTGTTTGAA ACGGATAGTG AAGAGGAGGA AGAAGAGGAA
    CTCAAAAAGG AGGATGAAGA ACCACCACGG AAGTCTGCAT TCCAGTTCGT CTACCAAGCC
    TGGATTACCG ATCCTAAAAC AGCACTCCGA CAGAGGAGGA AAGAGAAGAA GAAAATGGCC
    AGAGAAGAGC AGAAGGGAAG ACGCAAAGGA TCTGGGGAGG GTCCAGTGGA ATGGGAAGAC
    AGAGAAGATG AGCCAATCAA AAAGAAATCT GATGGACCAG ATAATATCAT CAAGAGGATA
    TTTAATATCC TGAAGTTCAC CTGGGTCCTG TTCCTGGCCA CAGTGGACAG TTTCACTACT
    TGGCTCAACT CCATCTCAAG AGAGCATATC GACATATCCA CAGTTCTGCG GATTGAAAGA
    TGCATGCTGA CCCGAGAGAT AAAGAAGGGC AACGTTCCGA CACGGGAAAG CATCCACATG
    TACTACCAGA ACCACATGAT GAACCTTTCC AGAGAATCCG GACTGGACAC AATCGACGAG
    CACTCTGGCG CTGGTTCCAG GGCCCAGGCA GCACACAGGA TGGATAGCTT AGATTCCCAT
    GACAGTATCT CCAGCTGTTA CACTGAAGCA ACCCTGCTGA TCTCAAGGCA GTCCACCCTT
    GATGATTTAG ATGGACAAGA CCCTGTTCCT AAAACAAGCG AGCGTGCTCG GCCTAGGCTC
    CGTAAAGTGT TCAGCATGGA CATGTCCTCC TCATCAGCTG ACAGTGGCAG TGTAGCATCA
    AGCGAGCCCA CACAGTGCAC CATGCTGTAT TCACGCCAAG GAACTACGGA AACCATTGAG
    GAGGTAGAGG CTGAAGCTGA GGAGGAGGTG GTAGAGAGTC TGGAGCCTGA GCTCCATGAT
    GCCGAAGAGA AGGAATATGC AGCTGACTAT GAAGCAGGGG TAGAGGAGGT CAGCCTCACT
    CCAGATGAGG AGTTGCCTCA GTTCTCAACA GATGACGGTG ACGTGGAGGC CCCGCCCTCC
    TACAGCAAGG CTGTCAGCTT TGAGCACCTC TCGTTTGCAT CACAAGATGA CTCCGGGGGC
    AAGAACCACA TGGTGGTCAG CCCTGACGAC AGCCGCACAG ACAAGCTGGA GTCCAGTATC
    TTACCGCCCC TGACTCACGA GCTGACAGCC AGCGATCTGC TCATGAGCAA GATGTTCCGT
    GATGATGAGC TTGAAGAATC TGAGAAATTC TACGTGGACC AGCCCCGGTT CTTGCTGCTA
    TTCTATGCCA TGTATAACAC TCTGGTAGCC CGCTCGGAAA TGGTGTGCTA TTTCGTGATC
    ATCCTGAACC ACATGATCTC TGCCTCCATC ATTACTCTTC TGTTGCCCAT CCTGATCTTC
    CTCTGGGCCA TGCTGTCCGT CCCCAGGCCC AGCCGGCGCT TCTGGATGAT GGCCATCGTC
    TACACTGAGG TGGCGATTGT AGTGAAATAT TTCTTCCAGT TTGGCTTCTT TCCTTGGAAT
    AAGGACTTGG AAATTTACAA GGACAGGCCC TACTTTCCAC CAAACATCAT AGGGGTGGAA
    AAGAAAGAGG GCTATGTTCT TTACGACCTC ATTCAGCTGC TGGCTCTGTT CTTCCATCGA
    TCCATTCTGA AGTGTCATGG CCTATGGGAC GAGGATGACA TTGTTGACAG TAACACAGAC
    AAGGAAGGAT CTGATGATGA GCTGTCCCTC GGTCAGGGCA GAAGAGGCTC CTCAGAGTCA
    CTGAAGTCCA TCAACTTGGC TGCCTCTGTA GAGTCCGTCC ATGTGACCTT CCCAGAGCAG
    CCAGCAGCCA TCCGGAGAAA ACGCTCCTGC AGCAGCTCGC AGATCTCCCC ACGGTCCAGC
    TTCTCTTCAA ATAGGTCCAA AAGAGGCAGC ACAAGCACCC GAAACAGCAG CCAAAAAGGA
    AGCAGTGTTT TGAGCATTAA ACAAAAAAGC AAAAGAGAAC TTTACATGGA AAAGCTTCAA
    GAACATTTAA TCAAAGCAAA GGCCTTTACC ATAAAGAAAA CTCTACAAAT CTATGTACCC
    ATTCGGCAAT TCTTCTATGA CCTCATCCAC CCAGACTACA GTGCTGTGAC TGACGTGTAT
    GTGCTGATGT TCCTGGCCGA CACCGTGGAC TTCATCATCA TCGTCTTTGG CTTCTGGGCT
    TTTGGGAAAC ACTCGGCAGC TGCAGATATC ACCTCTTCCC TATCAGAAGA CCAGGTCCCC
    GGGCCATTCC TGGTGATGGT TCTCATTCAG TTTGGAACCA TGGTGGTGGA CCGTGCACTC
    TACCTCAGGA AGACTGTGCT TGGCAAGGTC ATTTTCCAGG TCATCCTCGT GTTTGGAATT
    CACTTCTGGA TGTTCTTCAT CCTACCCAGT GTGACTGAGA GGAAATTCAG CCAGAACCTG
    GTGGCACAGC TCTGGTACTT TGTGAAATGT GTGTACTTTG GGTTGTCTGC CTACCAAATC
    CGCTGTGGCT ACCCAACACG TGTGCTCGGA AACTTCCTCA CGAAGAGCTA CAATTATGTC
    AACCTCTTCT TGTTTCAAGG GTTTCGTCTC GTCCCATTTC TGACAGAGCT GCGGGCTGTG
    ATGGACTGGG TGTGGACAGA TACAACCTTG AGCCTCTCCA GCTGGATTTG TGTGGAGGAC
    ATTTACGCAC ACATTTTCAT CTTAAAGTGC TGGCGAGAGT CGGAAAAGAG ATACCCTCAA
    CCCAGGGGCC AGAAGAAGAA GAAAGCTGTG AAGTATGGCA TGGGCGGAAT GATCATTGTC
    CTGCTCATCT GTATCGTCTG GTTTCCTCTG CTCTTCATGT CTTTGATCAA GTCTGTCGCA
    GGGGTTATTA ACCAACCCCT GGATGTGTCC GTCACAATAA CCCTGGGAGG CTATCAGCCT
    ATCTTCACAA TGAGTGCCCA GCAAAGCCAG CTGAAAGTTA TGGATGGTAC AAAGTATAAC
    GAATTCATAA AGTCTTTCGG CCCCAACAGT GGCGCTATGC AATTTCTGGA GAATTATGAA
    AGGCAAGACA TAACAGTAGC GGAACTGGAA GGGAATTCGA ATTCTTTGTG GACCATCAGC
    CCTCCCAGTA AGCAGAAAAT GATACAGGAG CTCACCGACC CCAATAGTTG CTTCTCTGTT
    GTTTTTTCAT GGAGTATCCA GAGAAACATG ACTCTGGGTG CAAAGGCTGA AATAGCAACG
    GACAAGCTTT CTTTTCCTCT CACGGATGCG ACACGAAGTA GCATAGCCAA AATGATTGCT
    GGCAATGACA CAGAAAGTTC CAACACACCA GTGACAATAG AAAAGATCTA CCCATACTAT
    GTGAAGGCAC CCAGTGACTC AAACTCAAAG CCTATAAAAC AGCTTCTGTC AGAAAACAAT
    TTCATGAATA TCACCATCAT TTTGTTCAGA GACAATGTCA CAAAGTCCAA TAGTGAGTGG
    TGGGTTCTCA ACCTGACTGG AAGTAGGATA TTCAATCAGG GATCCCAAGC CTTGGAGCTG
    GTGGTCTTCA ATGACAAAGT CAGCCCCCCA AGTCTGGGCT TCTTGGCTGG CTATGGCATC
    ATGGGATTGT ATGCATCCGT TGTCCTTGTA ATTGGGAAGT TTGTTCGCGA ATTCTTCAGT
    GGGATTTCTC ACTCTATCAT GTTTGAAGAG CTTCCAAATG TGGACAGAAT TTTGAAGTTA
    TGCACAGATA TATTTCTCGT ACGGGAGACA GGGGAACTGG AGCTGGAGGA AGACCTCTAT
    GCCAAATTGA TATTCCTGTA CAGGTCGCCA GAAACCATGA TCAAGTGGAC TAGAGAAAAA
    ACAAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa20915
    Clone ID Related Accession (Same CDS sequence) XM_225880.9 , XM_001063568.6
    Accession Version XM_225880.9 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8259bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product piezo-type mechanosensitive ion channel component 2 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005117.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_225880.8. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTCCCCAT CTGCCTGGCA 
    GTAGCATGTG CATTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCG ACGATGCAAG GACACACAGG GCGGCTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCCTTT CTGCTCCTTC ACATCATTTT CCACATCACA
    CTGGCCAGCC TGGAAGCTCA GCACCGCATC GCACCCGCTT ACAACTGCTC AACTTGGGAA
    AAGACCTTCC GGCAGATCGG CTTCGAAAGC TTGAAGGGAG CTGATGCTGG CAATGGCATC
    AGGGTGTTTG TGCCTGATAT CGGGATGTTC ATTGCGAGCC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACGGAA GAAGTAGCCC AATTTAATTC CGAGTGCGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GACTCAGAAG AAGCGCTGAT TTATGAGGAG
    GACTTAGATG GGGAAGAAGG CATGGAAGGC GAGCTGGAAG AAAGCACAAA GTTAAAAATG
    TTTCGCAGGC TCGCCTCCGT CGCTTCCAAG CTTAAGGAGT TCATCGGCAA TATGATAACC
    ACGGCGGGCA AGGTGGTGGT GACCATCCTG TTGGGCTCCT CTGGCATGAT GCTGCCATCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT CTGGGTCTGT GCACCTGGTG GTCCTGGTGC
    CGAACTTTTG ACCCATTGCT GTTTGGCTGC CTCTGTGTCC TGTTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTTCAGTTCT TCCAAGAAGC AGTCCCACCC
    AATGACTACT ATGCAAGGTT GTTTGGAATC AAATCCGTTA TTCAAACAGA CTGCTCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTACAC GCTGGCCACG CTGATCCGCA TTTGGTTGCA AGAGCCTCTT
    GTGCAGGAGG AGATGTCGAA GGAAGAGGAG GGTGCACTGG ATGGCTGCTC CAATCAGAAC
    ACAGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    CTTTTATCCA TGACCCAAGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACTGTGAAC
    GGCAACCCTG TGGACTACCA CACCATCCAC CCAAGTCTGC CCATAGAGAA TGGCCCCGCC
    AAGACGGACT TGTATACTAC TCCTCAGTAC CGGTGGGAGC CCTCAGAGGA GTCCTCAGAA
    AAGAAAGAGG AGGAAGAGGA CAAGAGAGAA GACTCAGAAG GGGAAGGGAG CCAAGAGGAA
    AAACGGAGTG TGAGAATTCA TGCCATGGTC GCCGTGTTCC AGTTCATCAT GAAGCAGAGT
    TACATCTGTG CCCTCATCGC CATGATGGCA TGGAGCATCA CCTACCACAG CTGGCTGACA
    TTTGTGCTGT TGATCTGGTC GTGCACTCTC TGGATGATTC GCAACAGGAG GAAGTACGCC
    ATGATCAGCT CTCCGTTCAT GGTTGTCTAT GCCAATCTGC TGCTGGTACT GCAGTACATA
    TGGAGCTTTG AACTTCCAGA AATCAAGAAA GTCCCTGGAT TTCTAGAAAA GAAAGAGCCT
    GGAGAGCTTG CCTCAAAGAT CCTCTTCACC ATCACATTTT GGCTGCTCCT GAGGCAGCAT
    CTCACAGAGC AGAAGGCCCT TCAGGAAAAG GAAGCACTTC TGTCTGAAGT CAAAATTGGA
    AGTCAAGAAC TTGACGAAAA GGATGAGGAA CTCCAAGATA TACAAGTGGA AGGAGAGCCC
    ATAGAGAAGG AAGAAGAGGA GGAGGAAATA AAGGAAGACA GACATGAGGT AAAGAAGGAA
    GAGGAAGAGG AAGAGGAAGA TGACGATGAC CAGGACATCA TGAAGGTTCT GGGCAATCTG
    GTGGTGGCCC TCTTCATCAA GTATTGGATC TACGTCTGCG GGGGCATGTT CTTTTTTGTC
    AGCTTTGAGG GGAAAATCGT CATGTACAAA ATCATCTACA TGGTGCTGTT CCTGTTCTGT
    GTGGCCTTGT ACCAGGTGCA CTACGAGTGG TGGAGGAAGA TCCTGAAATA CTTCTGGATG
    TCGGTGGTCA TTTACACCAT GCTGGTGCTC ATCTTCATCT ACACTTACCA GTTTGAGAAC
    TTCCCAGGCC TGTGGCAAAA CATGACCGGG TTAAAGAAAG AGAAGCTTGA GGATCTGGGC
    CTGAAGCAGT TCACTGTGGC CGAACTGTTC ACACGAATAT TCATCCCCAC CTCCTTTCTG
    CTGGTCTGCA TCCTGCACCT CCACTATTTC CATGACCGGT TCCTGGAACT CACAGACCTC
    AAGTCCATCC CCAGCAAAGA AGACAACACC ATCTACAGGT TAGCCCACCC TGAAGGAAGC
    CTCCCAGACC TAGCCATTAT GAACATGACG GCCAGCCTCG ATAAGCCTGA GAGGCAGAAG
    TTGGCAGAGC CTGGGGAGGA GAGGACAGAA GAATGTGCCA AGAAGGCAGA GAAGGGTGAG
    GCTGGGAAAG ACAGTGATGA GTCTGAGGAG GAGGAAGGTG AGGAAGAGGA ATCCGAGGAG
    GAAGAATCCT CAGATCTCAG GAACAAGTGG CACCTCGTGA TTGACCGGCT CACTGTGCTC
    TTTCTAAAGT TCCTGGAGTA TTTCCACAAG TTGCAGGTGT TTATGTGGTG GATCTTGGAG
    CTGCACATCA TCAAAATCGT CTCATCGTAC ATTATCTGGG TGACTGTCAA AGAGGTGTCT
    CTGTTCAACT ATGTATTTTT GATTTCTTGG GCATTTGCTC TGCCATATGC CAAACTCCGC
    CGTGTGGCTT CCAGTGTCTG CACTGTCTGG ACGTGTGTGA TCATCGTCTG CAAAATGTTG
    TACCAGCTGC AAACCATTAA GCCTGAGAAC TTTTCTGTCA ACTGCTCCTT GCCCAATGAA
    AATCAAACCA ACATACCCCT TAATGAGTTG AACAAGTCTC TTCTCTACAG TGCCCCTGTT
    GATCCCACTG AGTGGGTTGG CCTGAGGAAG TCCTCCCCTT TACTCGTCTA CCTGAGGAAC
    AACCTCCTGA TGTTGGCGAT TTTGGCCTTC GAAGTCACTG TTTACCGACA TCAGGAGTAC
    TATCGAGGCC GAAACAACTT GACTGCTCCG GTGTCTAAAA CTATCTTTCA TGACATCACA
    AGGTTACATC TAGATGATGG GCTTATCAAT TGTGCCAAAT ATTTTGTAAA CTACTTCTTC
    TACAAGTTTG GCCTAGAGAC CTGTTTCCTA ATGTCAGTGA ACGTAATTGG ACAGCGAATG
    GACTTCTACG CCATGATTCA TGCCTGTTGG CTGATCGGTG TCTTGTACCG TCGCAGAAGA
    AAAGCCATTG CTGAGGTCTG GCCCAAGTAC TGCTGCTTCC TGGCTTGCAT CATCACCTTC
    CAGTACTTTG TCTGCATAGG CATCCCTCCT GCCCCCTGCC GAGATTACCC ATGGAGGTTC
    AAGGGTGCCA ACTTCAATGA CAACATCATC AAGTGGCTGT ACTTCCCAGA TTTCATCGTG
    CGGCCCAACC CTGTGTTTCT TGTCTATGAC TTCATGCTGC TCCTGTGTGC TTCCTTACAA
    CGACAGATTT TTGAGGACGA AAACAAGGCA GCTGTGCGCA TCATGGCCGG GGACAATGTC
    GAGATTTGCA TGAACCTTGA CGCGGCCTCA TTCAGCCAGC ATAACCCTGT ACCAGATTTC
    ATTCACTGCA GATCTTACTT GGACATGTCC AAAGTGATCA TCTTCAGCTA CCTCTTCTGG
    TTCGTCCTCA CCATCATCTT CATTACCGGA ACGACCAGGA TCAGCATCTT TTGCATGGGT
    TACCTGGTGG CCTGTTTCTA TTTCCTGCTC TTTGGAGGTG ATCTGCTCCT GAAACCCATC
    AAGAGCATCC TACGCTACTG GGACTGGCTG ATTGCATACA ACGTGTTTGT GATCACCATG
    AAAAACATAC TGTCAATAGG AGCCTGTGGA TACATCGGTG CACTGGTGAG AAACAGCTGC
    TGGCTGATCC AAGCGTTCAG CCTGGCCTGC ACGGTGAAAG GCTATAAAAT GCCTGAAGAT
    GACTCCAGAT GCAAGCTGCC CAGTGGGGAG GCAGGGATCA TCTGGGACAG CATCTGCTTT
    GCGTTTCTCC TGCTACAGAG GAGAGTCTTC ATGAGTTACT ATTTCCTGCA TGTCGTGGCT
    GACATTAAAG CATCCCAGAT CCTGGCATCC AGAGGGGCCG AACTTTTCCA GGCTACAATT
    GTGAAAGCTG TAAAGGCAAG AATTGAAGAA GAGAAAAAAT CCATGGACCA GCTGAAGAGA
    CAGATGGATC GCATCAAAGC CCGGCAGCAA AAGTACAAAA AGGGTAAGGA GAGGATGCTG
    AGCTTGACCC AGGAGTCAGG GGAAGGCCAG GACATCCAGA AAGTCTCAGA AGAAGACGAT
    GAAAGAGAAG CAGACAAACA GAAAGCCAAG GGCAAAAAAA AGCAGTGGTG GCGGCCTTGG
    GTTGATCATG CTTCCATGGT CAGGAGTGGA GATTATTATT TGTTTGAAAC GGATAGTGAA
    GAGGAGGAAG AAGAGGAACT CAAAAAGGAG GATGAAGAAC CACCACGGAA GTCTGCATTC
    CAGTTCGTCT ACCAAGCCTG GATTACCGAT CCTAAAACAG CACTCCGACA GAGGAGGAAA
    GAGAAGAAGA AAATGGCCAG AGAAGAGCAG AAGGGAAGAC GCAAAGGATC TGGGGAGGGT
    CCAGTGGAAT GGGAAGACAG AGAAGATGAG CCAATCAAAA AGAAATCTGA TGGACCAGAT
    AATATCATCA AGAGGATATT TAATATCCTG AAGTTCACCT GGGTCCTGTT CCTGGCCACA
    GTGGACAGTT TCACTACTTG GCTCAACTCC ATCTCAAGAG AGCATATCGA CATATCCACA
    GTTCTGCGGA TTGAAAGATG CATGCTGACC CGAGAGATAA AGAAGGGCAA CGTTCCGACA
    CGGGAAAGCA TCCACATGTA CTACCAGAAC CACATGATGA ACCTTTCCAG AGAATCCGGA
    CTGGACACAA TCGACGAGCA CTCTGGCGCT GGTTCCAGGG CCCAGGCAGC ACACAGGATG
    GATAGCTTAG ATTCCCATGA CAGTATCTCC AGCGAGCCCA CACAGTGCAC CATGCTGTAT
    TCACGCCAAG GAACTACGGA AACCATTGAG GAGGTAGAGG CTGAAGCTGA GGAGGAGGTG
    GTAGAGAGTC TGGAGCCTGA GCTCCATGAT GCCGAAGAGA AGGAATATGC AGCTGACTAT
    GAAGCAGGGG TAGAGGAGGT CAGCCTCACT CCAGATGAGG AGTTGCCTCA GTTCTCAACA
    GATGACGGTG ACGTGGAGGC CCCGCCCTCC TACAGCAAGG CTGTCAGCTT TGAGCACCTC
    TCGTTTGCAT CACAAGATGA CTCCGGGGGC AAGAACCACA TGGTGGTCAG CCCTGACGAC
    AGCCGCACAG ACAAGCTGGA GTCCAGTATC TTACCGCCCC TGACTCACGA GCTGACAGCC
    AGCGATCTGC TCATGAGCAA GATGTTCCGT GATGATGAGC TTGAAGAATC TGAGAAATTC
    TACGTGGACC AGCCCCGGTT CTTGCTGCTA TTCTATGCCA TGTATAACAC TCTGGTAGCC
    CGCTCGGAAA TGGTGTGCTA TTTCGTGATC ATCCTGAACC ACATGATCTC TGCCTCCATC
    ATTACTCTTC TGTTGCCCAT CCTGATCTTC CTCTGGGCCA TGCTGTCCGT CCCCAGGCCC
    AGCCGGCGCT TCTGGATGAT GGCCATCGTC TACACTGAGG TGGCGATTGT AGTGAAATAT
    TTCTTCCAGT TTGGCTTCTT TCCTTGGAAT AAGGACTTGG AAATTTACAA GGACAGGCCC
    TACTTTCCAC CAAACATCAT AGGGGTGGAA AAGAAAGAGG GCTATGTTCT TTACGACCTC
    ATTCAGCTGC TGGCTCTGTT CTTCCATCGA TCCATTCTGA AGTGTCATGG CCTATGGGAC
    GAGGATGACA TTGTTGACAG TAACACAGAC AAGGAAGGAT CTGATGATGA GCTGTCCCTC
    GGTCAGGGCA GAAGAGGCTC CTCAGAGTCA CTGAAGTCCA TCAACTTGGC TGCCTCTGTA
    GAGTCCGTCC ATGTGACCTT CCCAGAGCAG CCAGCAGCCA TCCGGAGAAA ACGCTCCTGC
    AGCAGCTCGC AGATCTCCCC ACGGTCCAGC TTCTCTTCAA ATAGGTCCAA AAGAGGCAGC
    ACAAGCACCC GAAACAGCAG CCAAAAAGGA AGCAGTGTTT TGAGCATTAA ACAAAAAAGC
    AAAAGAGAAC TTTACATGGA AAAGCTTCAA GAACATTTAA TCAAAGCAAA GGCCTTTACC
    ATAAAGAAAA CTCTACAAAT CTATGTACCC ATTCGGCAAT TCTTCTATGA CCTCATCCAC
    CCAGACTACA GTGCTGTGAC TGACGTGTAT GTGCTGATGT TCCTGGCCGA CACCGTGGAC
    TTCATCATCA TCGTCTTTGG CTTCTGGGCT TTTGGGAAAC ACTCGGCAGC TGCAGATATC
    ACCTCTTCCC TATCAGAAGA CCAGGTCCCC GGGCCATTCC TGGTGATGGT TCTCATTCAG
    TTTGGAACCA TGGTGGTGGA CCGTGCACTC TACCTCAGGA AGACTGTGCT TGGCAAGGTC
    ATTTTCCAGG TCATCCTCGT GTTTGGAATT CACTTCTGGA TGTTCTTCAT CCTACCCAGT
    GTGACTGAGA GGAAATTCAG CCAGAACCTG GTGGCACAGC TCTGGTACTT TGTGAAATGT
    GTGTACTTTG GGTTGTCTGC CTACCAAATC CGCTGTGGCT ACCCAACACG TGTGCTCGGA
    AACTTCCTCA CGAAGAGCTA CAATTATGTC AACCTCTTCT TGTTTCAAGG GTTTCGTCTC
    GTCCCATTTC TGACAGAGCT GCGGGCTGTG ATGGACTGGG TGTGGACAGA TACAACCTTG
    AGCCTCTCCA GCTGGATTTG TGTGGAGGAC ATTTACGCAC ACATTTTCAT CTTAAAGTGC
    TGGCGAGAGT CGGAAAAGAG ATACCCTCAA CCCAGGGGCC AGAAGAAGAA GAAAGCTGTG
    AAGTATGGCA TGGGCGGAAT GATCATTGTC CTGCTCATCT GTATCGTCTG GTTTCCTCTG
    CTCTTCATGT CTTTGATCAA GTCTGTCGCA GGGGTTATTA ACCAACCCCT GGATGTGTCC
    GTCACAATAA CCCTGGGAGG CTATCAGCCT ATCTTCACAA TGAGTGCCCA GCAAAGCCAG
    CTGAAAGTTA TGGATGGTAC AAAGTATAAC GAATTCATAA AGTCTTTCGG CCCCAACAGT
    GGCGCTATGC AATTTCTGGA GAATTATGAA AGGCAAGACA TAACAGTAGC GGAACTGGAA
    GGGAATTCGA ATTCTTTGTG GACCATCAGC CCTCCCAGTA AGCAGAAAAT GATACAGGAG
    CTCACCGACC CCAATAGTTG CTTCTCTGTT GTTTTTTCAT GGAGTATCCA GAGAAACATG
    ACTCTGGGTG CAAAGGCTGA AATAGCAACG GACAAGCTTT CTTTTCCTCT CACGGATGCG
    ACACGAAGTA GCATAGCCAA AATGATTGCT GGCAATGACA CAGAAAGTTC CAACACACCA
    GTGACAATAG AAAAGATCTA CCCATACTAT GTGAAGGCAC CCAGTGACTC AAACTCAAAG
    CCTATAAAAC AGCTTCTGTC AGAAAACAAT TTCATGAATA TCACCATCAT TTTGTTCAGA
    GACAATGTCA CAAAGTCCAA TAGTGAGTGG TGGGTTCTCA ACCTGACTGG AAGTAGGATA
    TTCAATCAGG GATCCCAAGC CTTGGAGCTG GTGGTCTTCA ATGACAAAGT CAGCCCCCCA
    AGTCTGGGCT TCTTGGCTGG CTATGGCATC ATGGGATTGT ATGCATCCGT TGTCCTTGTA
    ATTGGGAAGT TTGTTCGCGA ATTCTTCAGT GGGATTTCTC ACTCTATCAT GTTTGAAGAG
    CTTCCAAATG TGGACAGAAT TTTGAAGTTA TGCACAGATA TATTTCTCGT ACGGGAGACA
    GGGGAACTGG AGCTGGAGGA AGACCTCTAT GCCAAATTGA TATTCCTGTA CAGGTCGCCA
    GAAACCATGA TCAAGTGGAC TAGAGAAAAA ACAAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_225880.5
    CDS940..9198
    Translation

    Target ORF information:

    RefSeq Version XM_225880.9
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_225880.9

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTCCCCAT CTGCCTGGCA 
    GTAGCATGTG CATTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCG ACGATGCAAG GACACACAGG GCGGCTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCCTTT CTGCTCCTTC ACATCATTTT CCACATCACA
    CTGGCCAGCC TGGAAGCTCA GCACCGCATC GCACCCGCTT ACAACTGCTC AACTTGGGAA
    AAGACCTTCC GGCAGATCGG CTTCGAAAGC TTGAAGGGAG CTGATGCTGG CAATGGCATC
    AGGGTGTTTG TGCCTGATAT CGGGATGTTC ATTGCGAGCC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACGGAA GAAGTAGCCC AATTTAATTC CGAGTGCGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GACTCAGAAG AAGCGCTGAT TTATGAGGAG
    GACTTAGATG GGGAAGAAGG CATGGAAGGC GAGCTGGAAG AAAGCACAAA GTTAAAAATG
    TTTCGCAGGC TCGCCTCCGT CGCTTCCAAG CTTAAGGAGT TCATCGGCAA TATGATAACC
    ACGGCGGGCA AGGTGGTGGT GACCATCCTG TTGGGCTCCT CTGGCATGAT GCTGCCATCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT CTGGGTCTGT GCACCTGGTG GTCCTGGTGC
    CGAACTTTTG ACCCATTGCT GTTTGGCTGC CTCTGTGTCC TGTTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTTCAGTTCT TCCAAGAAGC AGTCCCACCC
    AATGACTACT ATGCAAGGTT GTTTGGAATC AAATCCGTTA TTCAAACAGA CTGCTCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTACAC GCTGGCCACG CTGATCCGCA TTTGGTTGCA AGAGCCTCTT
    GTGCAGGAGG AGATGTCGAA GGAAGAGGAG GGTGCACTGG ATGGCTGCTC CAATCAGAAC
    ACAGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    CTTTTATCCA TGACCCAAGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACTGTGAAC
    GGCAACCCTG TGGACTACCA CACCATCCAC CCAAGTCTGC CCATAGAGAA TGGCCCCGCC
    AAGACGGACT TGTATACTAC TCCTCAGTAC CGGTGGGAGC CCTCAGAGGA GTCCTCAGAA
    AAGAAAGAGG AGGAAGAGGA CAAGAGAGAA GACTCAGAAG GGGAAGGGAG CCAAGAGGAA
    AAACGGAGTG TGAGAATTCA TGCCATGGTC GCCGTGTTCC AGTTCATCAT GAAGCAGAGT
    TACATCTGTG CCCTCATCGC CATGATGGCA TGGAGCATCA CCTACCACAG CTGGCTGACA
    TTTGTGCTGT TGATCTGGTC GTGCACTCTC TGGATGATTC GCAACAGGAG GAAGTACGCC
    ATGATCAGCT CTCCGTTCAT GGTTGTCTAT GCCAATCTGC TGCTGGTACT GCAGTACATA
    TGGAGCTTTG AACTTCCAGA AATCAAGAAA GTCCCTGGAT TTCTAGAAAA GAAAGAGCCT
    GGAGAGCTTG CCTCAAAGAT CCTCTTCACC ATCACATTTT GGCTGCTCCT GAGGCAGCAT
    CTCACAGAGC AGAAGGCCCT TCAGGAAAAG GAAGCACTTC TGTCTGAAGT CAAAATTGGA
    AGTCAAGAAC TTGACGAAAA GGATGAGGAA CTCCAAGATA TACAAGTGGA AGGAGAGCCC
    ATAGAGAAGG AAGAAGAGGA GGAGGAAATA AAGGAAGACA GACATGAGGT AAAGAAGGAA
    GAGGAAGAGG AAGAGGAAGA TGACGATGAC CAGGACATCA TGAAGGTTCT GGGCAATCTG
    GTGGTGGCCC TCTTCATCAA GTATTGGATC TACGTCTGCG GGGGCATGTT CTTTTTTGTC
    AGCTTTGAGG GGAAAATCGT CATGTACAAA ATCATCTACA TGGTGCTGTT CCTGTTCTGT
    GTGGCCTTGT ACCAGGTGCA CTACGAGTGG TGGAGGAAGA TCCTGAAATA CTTCTGGATG
    TCGGTGGTCA TTTACACCAT GCTGGTGCTC ATCTTCATCT ACACTTACCA GTTTGAGAAC
    TTCCCAGGCC TGTGGCAAAA CATGACCGGG TTAAAGAAAG AGAAGCTTGA GGATCTGGGC
    CTGAAGCAGT TCACTGTGGC CGAACTGTTC ACACGAATAT TCATCCCCAC CTCCTTTCTG
    CTGGTCTGCA TCCTGCACCT CCACTATTTC CATGACCGGT TCCTGGAACT CACAGACCTC
    AAGTCCATCC CCAGCAAAGA AGACAACACC ATCTACAGGT TAGCCCACCC TGAAGGAAGC
    CTCCCAGACC TAGCCATTAT GAACATGACG GCCAGCCTCG ATAAGCCTGA GAGGCAGAAG
    TTGGCAGAGC CTGGGGAGGA GAGGACAGAA GAATGTGCCA AGAAGGCAGA GAAGGGTGAG
    GCTGGGAAAG ACAGTGATGA GTCTGAGGAG GAGGAAGGTG AGGAAGAGGA ATCCGAGGAG
    GAAGAATCCT CAGATCTCAG GAACAAGTGG CACCTCGTGA TTGACCGGCT CACTGTGCTC
    TTTCTAAAGT TCCTGGAGTA TTTCCACAAG TTGCAGGTGT TTATGTGGTG GATCTTGGAG
    CTGCACATCA TCAAAATCGT CTCATCGTAC ATTATCTGGG TGACTGTCAA AGAGGTGTCT
    CTGTTCAACT ATGTATTTTT GATTTCTTGG GCATTTGCTC TGCCATATGC CAAACTCCGC
    CGTGTGGCTT CCAGTGTCTG CACTGTCTGG ACGTGTGTGA TCATCGTCTG CAAAATGTTG
    TACCAGCTGC AAACCATTAA GCCTGAGAAC TTTTCTGTCA ACTGCTCCTT GCCCAATGAA
    AATCAAACCA ACATACCCCT TAATGAGTTG AACAAGTCTC TTCTCTACAG TGCCCCTGTT
    GATCCCACTG AGTGGGTTGG CCTGAGGAAG TCCTCCCCTT TACTCGTCTA CCTGAGGAAC
    AACCTCCTGA TGTTGGCGAT TTTGGCCTTC GAAGTCACTG TTTACCGACA TCAGGAGTAC
    TATCGAGGCC GAAACAACTT GACTGCTCCG GTGTCTAAAA CTATCTTTCA TGACATCACA
    AGGTTACATC TAGATGATGG GCTTATCAAT TGTGCCAAAT ATTTTGTAAA CTACTTCTTC
    TACAAGTTTG GCCTAGAGAC CTGTTTCCTA ATGTCAGTGA ACGTAATTGG ACAGCGAATG
    GACTTCTACG CCATGATTCA TGCCTGTTGG CTGATCGGTG TCTTGTACCG TCGCAGAAGA
    AAAGCCATTG CTGAGGTCTG GCCCAAGTAC TGCTGCTTCC TGGCTTGCAT CATCACCTTC
    CAGTACTTTG TCTGCATAGG CATCCCTCCT GCCCCCTGCC GAGATTACCC ATGGAGGTTC
    AAGGGTGCCA ACTTCAATGA CAACATCATC AAGTGGCTGT ACTTCCCAGA TTTCATCGTG
    CGGCCCAACC CTGTGTTTCT TGTCTATGAC TTCATGCTGC TCCTGTGTGC TTCCTTACAA
    CGACAGATTT TTGAGGACGA AAACAAGGCA GCTGTGCGCA TCATGGCCGG GGACAATGTC
    GAGATTTGCA TGAACCTTGA CGCGGCCTCA TTCAGCCAGC ATAACCCTGT ACCAGATTTC
    ATTCACTGCA GATCTTACTT GGACATGTCC AAAGTGATCA TCTTCAGCTA CCTCTTCTGG
    TTCGTCCTCA CCATCATCTT CATTACCGGA ACGACCAGGA TCAGCATCTT TTGCATGGGT
    TACCTGGTGG CCTGTTTCTA TTTCCTGCTC TTTGGAGGTG ATCTGCTCCT GAAACCCATC
    AAGAGCATCC TACGCTACTG GGACTGGCTG ATTGCATACA ACGTGTTTGT GATCACCATG
    AAAAACATAC TGTCAATAGG AGCCTGTGGA TACATCGGTG CACTGGTGAG AAACAGCTGC
    TGGCTGATCC AAGCGTTCAG CCTGGCCTGC ACGGTGAAAG GCTATAAAAT GCCTGAAGAT
    GACTCCAGAT GCAAGCTGCC CAGTGGGGAG GCAGGGATCA TCTGGGACAG CATCTGCTTT
    GCGTTTCTCC TGCTACAGAG GAGAGTCTTC ATGAGTTACT ATTTCCTGCA TGTCGTGGCT
    GACATTAAAG CATCCCAGAT CCTGGCATCC AGAGGGGCCG AACTTTTCCA GGCTACAATT
    GTGAAAGCTG TAAAGGCAAG AATTGAAGAA GAGAAAAAAT CCATGGACCA GCTGAAGAGA
    CAGATGGATC GCATCAAAGC CCGGCAGCAA AAGTACAAAA AGGGTAAGGA GAGGATGCTG
    AGCTTGACCC AGGAGTCAGG GGAAGGCCAG GACATCCAGA AAGTCTCAGA AGAAGACGAT
    GAAAGAGAAG CAGACAAACA GAAAGCCAAG GGCAAAAAAA AGCAGTGGTG GCGGCCTTGG
    GTTGATCATG CTTCCATGGT CAGGAGTGGA GATTATTATT TGTTTGAAAC GGATAGTGAA
    GAGGAGGAAG AAGAGGAACT CAAAAAGGAG GATGAAGAAC CACCACGGAA GTCTGCATTC
    CAGTTCGTCT ACCAAGCCTG GATTACCGAT CCTAAAACAG CACTCCGACA GAGGAGGAAA
    GAGAAGAAGA AAATGGCCAG AGAAGAGCAG AAGGGAAGAC GCAAAGGATC TGGGGAGGGT
    CCAGTGGAAT GGGAAGACAG AGAAGATGAG CCAATCAAAA AGAAATCTGA TGGACCAGAT
    AATATCATCA AGAGGATATT TAATATCCTG AAGTTCACCT GGGTCCTGTT CCTGGCCACA
    GTGGACAGTT TCACTACTTG GCTCAACTCC ATCTCAAGAG AGCATATCGA CATATCCACA
    GTTCTGCGGA TTGAAAGATG CATGCTGACC CGAGAGATAA AGAAGGGCAA CGTTCCGACA
    CGGGAAAGCA TCCACATGTA CTACCAGAAC CACATGATGA ACCTTTCCAG AGAATCCGGA
    CTGGACACAA TCGACGAGCA CTCTGGCGCT GGTTCCAGGG CCCAGGCAGC ACACAGGATG
    GATAGCTTAG ATTCCCATGA CAGTATCTCC AGCGAGCCCA CACAGTGCAC CATGCTGTAT
    TCACGCCAAG GAACTACGGA AACCATTGAG GAGGTAGAGG CTGAAGCTGA GGAGGAGGTG
    GTAGAGAGTC TGGAGCCTGA GCTCCATGAT GCCGAAGAGA AGGAATATGC AGCTGACTAT
    GAAGCAGGGG TAGAGGAGGT CAGCCTCACT CCAGATGAGG AGTTGCCTCA GTTCTCAACA
    GATGACGGTG ACGTGGAGGC CCCGCCCTCC TACAGCAAGG CTGTCAGCTT TGAGCACCTC
    TCGTTTGCAT CACAAGATGA CTCCGGGGGC AAGAACCACA TGGTGGTCAG CCCTGACGAC
    AGCCGCACAG ACAAGCTGGA GTCCAGTATC TTACCGCCCC TGACTCACGA GCTGACAGCC
    AGCGATCTGC TCATGAGCAA GATGTTCCGT GATGATGAGC TTGAAGAATC TGAGAAATTC
    TACGTGGACC AGCCCCGGTT CTTGCTGCTA TTCTATGCCA TGTATAACAC TCTGGTAGCC
    CGCTCGGAAA TGGTGTGCTA TTTCGTGATC ATCCTGAACC ACATGATCTC TGCCTCCATC
    ATTACTCTTC TGTTGCCCAT CCTGATCTTC CTCTGGGCCA TGCTGTCCGT CCCCAGGCCC
    AGCCGGCGCT TCTGGATGAT GGCCATCGTC TACACTGAGG TGGCGATTGT AGTGAAATAT
    TTCTTCCAGT TTGGCTTCTT TCCTTGGAAT AAGGACTTGG AAATTTACAA GGACAGGCCC
    TACTTTCCAC CAAACATCAT AGGGGTGGAA AAGAAAGAGG GCTATGTTCT TTACGACCTC
    ATTCAGCTGC TGGCTCTGTT CTTCCATCGA TCCATTCTGA AGTGTCATGG CCTATGGGAC
    GAGGATGACA TTGTTGACAG TAACACAGAC AAGGAAGGAT CTGATGATGA GCTGTCCCTC
    GGTCAGGGCA GAAGAGGCTC CTCAGAGTCA CTGAAGTCCA TCAACTTGGC TGCCTCTGTA
    GAGTCCGTCC ATGTGACCTT CCCAGAGCAG CCAGCAGCCA TCCGGAGAAA ACGCTCCTGC
    AGCAGCTCGC AGATCTCCCC ACGGTCCAGC TTCTCTTCAA ATAGGTCCAA AAGAGGCAGC
    ACAAGCACCC GAAACAGCAG CCAAAAAGGA AGCAGTGTTT TGAGCATTAA ACAAAAAAGC
    AAAAGAGAAC TTTACATGGA AAAGCTTCAA GAACATTTAA TCAAAGCAAA GGCCTTTACC
    ATAAAGAAAA CTCTACAAAT CTATGTACCC ATTCGGCAAT TCTTCTATGA CCTCATCCAC
    CCAGACTACA GTGCTGTGAC TGACGTGTAT GTGCTGATGT TCCTGGCCGA CACCGTGGAC
    TTCATCATCA TCGTCTTTGG CTTCTGGGCT TTTGGGAAAC ACTCGGCAGC TGCAGATATC
    ACCTCTTCCC TATCAGAAGA CCAGGTCCCC GGGCCATTCC TGGTGATGGT TCTCATTCAG
    TTTGGAACCA TGGTGGTGGA CCGTGCACTC TACCTCAGGA AGACTGTGCT TGGCAAGGTC
    ATTTTCCAGG TCATCCTCGT GTTTGGAATT CACTTCTGGA TGTTCTTCAT CCTACCCAGT
    GTGACTGAGA GGAAATTCAG CCAGAACCTG GTGGCACAGC TCTGGTACTT TGTGAAATGT
    GTGTACTTTG GGTTGTCTGC CTACCAAATC CGCTGTGGCT ACCCAACACG TGTGCTCGGA
    AACTTCCTCA CGAAGAGCTA CAATTATGTC AACCTCTTCT TGTTTCAAGG GTTTCGTCTC
    GTCCCATTTC TGACAGAGCT GCGGGCTGTG ATGGACTGGG TGTGGACAGA TACAACCTTG
    AGCCTCTCCA GCTGGATTTG TGTGGAGGAC ATTTACGCAC ACATTTTCAT CTTAAAGTGC
    TGGCGAGAGT CGGAAAAGAG ATACCCTCAA CCCAGGGGCC AGAAGAAGAA GAAAGCTGTG
    AAGTATGGCA TGGGCGGAAT GATCATTGTC CTGCTCATCT GTATCGTCTG GTTTCCTCTG
    CTCTTCATGT CTTTGATCAA GTCTGTCGCA GGGGTTATTA ACCAACCCCT GGATGTGTCC
    GTCACAATAA CCCTGGGAGG CTATCAGCCT ATCTTCACAA TGAGTGCCCA GCAAAGCCAG
    CTGAAAGTTA TGGATGGTAC AAAGTATAAC GAATTCATAA AGTCTTTCGG CCCCAACAGT
    GGCGCTATGC AATTTCTGGA GAATTATGAA AGGCAAGACA TAACAGTAGC GGAACTGGAA
    GGGAATTCGA ATTCTTTGTG GACCATCAGC CCTCCCAGTA AGCAGAAAAT GATACAGGAG
    CTCACCGACC CCAATAGTTG CTTCTCTGTT GTTTTTTCAT GGAGTATCCA GAGAAACATG
    ACTCTGGGTG CAAAGGCTGA AATAGCAACG GACAAGCTTT CTTTTCCTCT CACGGATGCG
    ACACGAAGTA GCATAGCCAA AATGATTGCT GGCAATGACA CAGAAAGTTC CAACACACCA
    GTGACAATAG AAAAGATCTA CCCATACTAT GTGAAGGCAC CCAGTGACTC AAACTCAAAG
    CCTATAAAAC AGCTTCTGTC AGAAAACAAT TTCATGAATA TCACCATCAT TTTGTTCAGA
    GACAATGTCA CAAAGTCCAA TAGTGAGTGG TGGGTTCTCA ACCTGACTGG AAGTAGGATA
    TTCAATCAGG GATCCCAAGC CTTGGAGCTG GTGGTCTTCA ATGACAAAGT CAGCCCCCCA
    AGTCTGGGCT TCTTGGCTGG CTATGGCATC ATGGGATTGT ATGCATCCGT TGTCCTTGTA
    ATTGGGAAGT TTGTTCGCGA ATTCTTCAGT GGGATTTCTC ACTCTATCAT GTTTGAAGAG
    CTTCCAAATG TGGACAGAAT TTTGAAGTTA TGCACAGATA TATTTCTCGT ACGGGAGACA
    GGGGAACTGG AGCTGGAGGA AGACCTCTAT GCCAAATTGA TATTCCTGTA CAGGTCGCCA
    GAAACCATGA TCAAGTGGAC TAGAGAAAAA ACAAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa20915
    Clone ID Related Accession (Same CDS sequence) XM_225880.9 , XM_001063568.6
    Accession Version XM_001063568.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8259bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product piezo-type mechanosensitive ion channel component 2 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000086.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_001063568.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTCCCCAT CTGCCTGGCA 
    GTAGCATGTG CATTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCG ACGATGCAAG GACACACAGG GCGGCTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCCTTT CTGCTCCTTC ACATCATTTT CCACATCACA
    CTGGCCAGCC TGGAAGCTCA GCACCGCATC GCACCCGCTT ACAACTGCTC AACTTGGGAA
    AAGACCTTCC GGCAGATCGG CTTCGAAAGC TTGAAGGGAG CTGATGCTGG CAATGGCATC
    AGGGTGTTTG TGCCTGATAT CGGGATGTTC ATTGCGAGCC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACGGAA GAAGTAGCCC AATTTAATTC CGAGTGCGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GACTCAGAAG AAGCGCTGAT TTATGAGGAG
    GACTTAGATG GGGAAGAAGG CATGGAAGGC GAGCTGGAAG AAAGCACAAA GTTAAAAATG
    TTTCGCAGGC TCGCCTCCGT CGCTTCCAAG CTTAAGGAGT TCATCGGCAA TATGATAACC
    ACGGCGGGCA AGGTGGTGGT GACCATCCTG TTGGGCTCCT CTGGCATGAT GCTGCCATCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT CTGGGTCTGT GCACCTGGTG GTCCTGGTGC
    CGAACTTTTG ACCCATTGCT GTTTGGCTGC CTCTGTGTCC TGTTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTTCAGTTCT TCCAAGAAGC AGTCCCACCC
    AATGACTACT ATGCAAGGTT GTTTGGAATC AAATCCGTTA TTCAAACAGA CTGCTCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTACAC GCTGGCCACG CTGATCCGCA TTTGGTTGCA AGAGCCTCTT
    GTGCAGGAGG AGATGTCGAA GGAAGAGGAG GGTGCACTGG ATGGCTGCTC CAATCAGAAC
    ACAGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    CTTTTATCCA TGACCCAAGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACTGTGAAC
    GGCAACCCTG TGGACTACCA CACCATCCAC CCAAGTCTGC CCATAGAGAA TGGCCCCGCC
    AAGACGGACT TGTATACTAC TCCTCAGTAC CGGTGGGAGC CCTCAGAGGA GTCCTCAGAA
    AAGAAAGAGG AGGAAGAGGA CAAGAGAGAA GACTCAGAAG GGGAAGGGAG CCAAGAGGAA
    AAACGGAGTG TGAGAATTCA TGCCATGGTC GCCGTGTTCC AGTTCATCAT GAAGCAGAGT
    TACATCTGTG CCCTCATCGC CATGATGGCA TGGAGCATCA CCTACCACAG CTGGCTGACA
    TTTGTGCTGT TGATCTGGTC GTGCACTCTC TGGATGATTC GCAACAGGAG GAAGTACGCC
    ATGATCAGCT CTCCGTTCAT GGTTGTCTAT GCCAATCTGC TGCTGGTACT GCAGTACATA
    TGGAGCTTTG AACTTCCAGA AATCAAGAAA GTCCCTGGAT TTCTAGAAAA GAAAGAGCCT
    GGAGAGCTTG CCTCAAAGAT CCTCTTCACC ATCACATTTT GGCTGCTCCT GAGGCAGCAT
    CTCACAGAGC AGAAGGCCCT TCAGGAAAAG GAAGCACTTC TGTCTGAAGT CAAAATTGGA
    AGTCAAGAAC TTGACGAAAA GGATGAGGAA CTCCAAGATA TACAAGTGGA AGGAGAGCCC
    ATAGAGAAGG AAGAAGAGGA GGAGGAAATA AAGGAAGACA GACATGAGGT AAAGAAGGAA
    GAGGAAGAGG AAGAGGAAGA TGACGATGAC CAGGACATCA TGAAGGTTCT GGGCAATCTG
    GTGGTGGCCC TCTTCATCAA GTATTGGATC TACGTCTGCG GGGGCATGTT CTTTTTTGTC
    AGCTTTGAGG GGAAAATCGT CATGTACAAA ATCATCTACA TGGTGCTGTT CCTGTTCTGT
    GTGGCCTTGT ACCAGGTGCA CTACGAGTGG TGGAGGAAGA TCCTGAAATA CTTCTGGATG
    TCGGTGGTCA TTTACACCAT GCTGGTGCTC ATCTTCATCT ACACTTACCA GTTTGAGAAC
    TTCCCAGGCC TGTGGCAAAA CATGACCGGG TTAAAGAAAG AGAAGCTTGA GGATCTGGGC
    CTGAAGCAGT TCACTGTGGC CGAACTGTTC ACACGAATAT TCATCCCCAC CTCCTTTCTG
    CTGGTCTGCA TCCTGCACCT CCACTATTTC CATGACCGGT TCCTGGAACT CACAGACCTC
    AAGTCCATCC CCAGCAAAGA AGACAACACC ATCTACAGGT TAGCCCACCC TGAAGGAAGC
    CTCCCAGACC TAGCCATTAT GAACATGACG GCCAGCCTCG ATAAGCCTGA GAGGCAGAAG
    TTGGCAGAGC CTGGGGAGGA GAGGACAGAA GAATGTGCCA AGAAGGCAGA GAAGGGTGAG
    GCTGGGAAAG ACAGTGATGA GTCTGAGGAG GAGGAAGGTG AGGAAGAGGA ATCCGAGGAG
    GAAGAATCCT CAGATCTCAG GAACAAGTGG CACCTCGTGA TTGACCGGCT CACTGTGCTC
    TTTCTAAAGT TCCTGGAGTA TTTCCACAAG TTGCAGGTGT TTATGTGGTG GATCTTGGAG
    CTGCACATCA TCAAAATCGT CTCATCGTAC ATTATCTGGG TGACTGTCAA AGAGGTGTCT
    CTGTTCAACT ATGTATTTTT GATTTCTTGG GCATTTGCTC TGCCATATGC CAAACTCCGC
    CGTGTGGCTT CCAGTGTCTG CACTGTCTGG ACGTGTGTGA TCATCGTCTG CAAAATGTTG
    TACCAGCTGC AAACCATTAA GCCTGAGAAC TTTTCTGTCA ACTGCTCCTT GCCCAATGAA
    AATCAAACCA ACATACCCCT TAATGAGTTG AACAAGTCTC TTCTCTACAG TGCCCCTGTT
    GATCCCACTG AGTGGGTTGG CCTGAGGAAG TCCTCCCCTT TACTCGTCTA CCTGAGGAAC
    AACCTCCTGA TGTTGGCGAT TTTGGCCTTC GAAGTCACTG TTTACCGACA TCAGGAGTAC
    TATCGAGGCC GAAACAACTT GACTGCTCCG GTGTCTAAAA CTATCTTTCA TGACATCACA
    AGGTTACATC TAGATGATGG GCTTATCAAT TGTGCCAAAT ATTTTGTAAA CTACTTCTTC
    TACAAGTTTG GCCTAGAGAC CTGTTTCCTA ATGTCAGTGA ACGTAATTGG ACAGCGAATG
    GACTTCTACG CCATGATTCA TGCCTGTTGG CTGATCGGTG TCTTGTACCG TCGCAGAAGA
    AAAGCCATTG CTGAGGTCTG GCCCAAGTAC TGCTGCTTCC TGGCTTGCAT CATCACCTTC
    CAGTACTTTG TCTGCATAGG CATCCCTCCT GCCCCCTGCC GAGATTACCC ATGGAGGTTC
    AAGGGTGCCA ACTTCAATGA CAACATCATC AAGTGGCTGT ACTTCCCAGA TTTCATCGTG
    CGGCCCAACC CTGTGTTTCT TGTCTATGAC TTCATGCTGC TCCTGTGTGC TTCCTTACAA
    CGACAGATTT TTGAGGACGA AAACAAGGCA GCTGTGCGCA TCATGGCCGG GGACAATGTC
    GAGATTTGCA TGAACCTTGA CGCGGCCTCA TTCAGCCAGC ATAACCCTGT ACCAGATTTC
    ATTCACTGCA GATCTTACTT GGACATGTCC AAAGTGATCA TCTTCAGCTA CCTCTTCTGG
    TTCGTCCTCA CCATCATCTT CATTACCGGA ACGACCAGGA TCAGCATCTT TTGCATGGGT
    TACCTGGTGG CCTGTTTCTA TTTCCTGCTC TTTGGAGGTG ATCTGCTCCT GAAACCCATC
    AAGAGCATCC TACGCTACTG GGACTGGCTG ATTGCATACA ACGTGTTTGT GATCACCATG
    AAAAACATAC TGTCAATAGG AGCCTGTGGA TACATCGGTG CACTGGTGAG AAACAGCTGC
    TGGCTGATCC AAGCGTTCAG CCTGGCCTGC ACGGTGAAAG GCTATAAAAT GCCTGAAGAT
    GACTCCAGAT GCAAGCTGCC CAGTGGGGAG GCAGGGATCA TCTGGGACAG CATCTGCTTT
    GCGTTTCTCC TGCTACAGAG GAGAGTCTTC ATGAGTTACT ATTTCCTGCA TGTCGTGGCT
    GACATTAAAG CATCCCAGAT CCTGGCATCC AGAGGGGCCG AACTTTTCCA GGCTACAATT
    GTGAAAGCTG TAAAGGCAAG AATTGAAGAA GAGAAAAAAT CCATGGACCA GCTGAAGAGA
    CAGATGGATC GCATCAAAGC CCGGCAGCAA AAGTACAAAA AGGGTAAGGA GAGGATGCTG
    AGCTTGACCC AGGAGTCAGG GGAAGGCCAG GACATCCAGA AAGTCTCAGA AGAAGACGAT
    GAAAGAGAAG CAGACAAACA GAAAGCCAAG GGCAAAAAAA AGCAGTGGTG GCGGCCTTGG
    GTTGATCATG CTTCCATGGT CAGGAGTGGA GATTATTATT TGTTTGAAAC GGATAGTGAA
    GAGGAGGAAG AAGAGGAACT CAAAAAGGAG GATGAAGAAC CACCACGGAA GTCTGCATTC
    CAGTTCGTCT ACCAAGCCTG GATTACCGAT CCTAAAACAG CACTCCGACA GAGGAGGAAA
    GAGAAGAAGA AAATGGCCAG AGAAGAGCAG AAGGGAAGAC GCAAAGGATC TGGGGAGGGT
    CCAGTGGAAT GGGAAGACAG AGAAGATGAG CCAATCAAAA AGAAATCTGA TGGACCAGAT
    AATATCATCA AGAGGATATT TAATATCCTG AAGTTCACCT GGGTCCTGTT CCTGGCCACA
    GTGGACAGTT TCACTACTTG GCTCAACTCC ATCTCAAGAG AGCATATCGA CATATCCACA
    GTTCTGCGGA TTGAAAGATG CATGCTGACC CGAGAGATAA AGAAGGGCAA CGTTCCGACA
    CGGGAAAGCA TCCACATGTA CTACCAGAAC CACATGATGA ACCTTTCCAG AGAATCCGGA
    CTGGACACAA TCGACGAGCA CTCTGGCGCT GGTTCCAGGG CCCAGGCAGC ACACAGGATG
    GATAGCTTAG ATTCCCATGA CAGTATCTCC AGCGAGCCCA CACAGTGCAC CATGCTGTAT
    TCACGCCAAG GAACTACGGA AACCATTGAG GAGGTAGAGG CTGAAGCTGA GGAGGAGGTG
    GTAGAGAGTC TGGAGCCTGA GCTCCATGAT GCCGAAGAGA AGGAATATGC AGCTGACTAT
    GAAGCAGGGG TAGAGGAGGT CAGCCTCACT CCAGATGAGG AGTTGCCTCA GTTCTCAACA
    GATGACGGTG ACGTGGAGGC CCCGCCCTCC TACAGCAAGG CTGTCAGCTT TGAGCACCTC
    TCGTTTGCAT CACAAGATGA CTCCGGGGGC AAGAACCACA TGGTGGTCAG CCCTGACGAC
    AGCCGCACAG ACAAGCTGGA GTCCAGTATC TTACCGCCCC TGACTCACGA GCTGACAGCC
    AGCGATCTGC TCATGAGCAA GATGTTCCGT GATGATGAGC TTGAAGAATC TGAGAAATTC
    TACGTGGACC AGCCCCGGTT CTTGCTGCTA TTCTATGCCA TGTATAACAC TCTGGTAGCC
    CGCTCGGAAA TGGTGTGCTA TTTCGTGATC ATCCTGAACC ACATGATCTC TGCCTCCATC
    ATTACTCTTC TGTTGCCCAT CCTGATCTTC CTCTGGGCCA TGCTGTCCGT CCCCAGGCCC
    AGCCGGCGCT TCTGGATGAT GGCCATCGTC TACACTGAGG TGGCGATTGT AGTGAAATAT
    TTCTTCCAGT TTGGCTTCTT TCCTTGGAAT AAGGACTTGG AAATTTACAA GGACAGGCCC
    TACTTTCCAC CAAACATCAT AGGGGTGGAA AAGAAAGAGG GCTATGTTCT TTACGACCTC
    ATTCAGCTGC TGGCTCTGTT CTTCCATCGA TCCATTCTGA AGTGTCATGG CCTATGGGAC
    GAGGATGACA TTGTTGACAG TAACACAGAC AAGGAAGGAT CTGATGATGA GCTGTCCCTC
    GGTCAGGGCA GAAGAGGCTC CTCAGAGTCA CTGAAGTCCA TCAACTTGGC TGCCTCTGTA
    GAGTCCGTCC ATGTGACCTT CCCAGAGCAG CCAGCAGCCA TCCGGAGAAA ACGCTCCTGC
    AGCAGCTCGC AGATCTCCCC ACGGTCCAGC TTCTCTTCAA ATAGGTCCAA AAGAGGCAGC
    ACAAGCACCC GAAACAGCAG CCAAAAAGGA AGCAGTGTTT TGAGCATTAA ACAAAAAAGC
    AAAAGAGAAC TTTACATGGA AAAGCTTCAA GAACATTTAA TCAAAGCAAA GGCCTTTACC
    ATAAAGAAAA CTCTACAAAT CTATGTACCC ATTCGGCAAT TCTTCTATGA CCTCATCCAC
    CCAGACTACA GTGCTGTGAC TGACGTGTAT GTGCTGATGT TCCTGGCCGA CACCGTGGAC
    TTCATCATCA TCGTCTTTGG CTTCTGGGCT TTTGGGAAAC ACTCGGCAGC TGCAGATATC
    ACCTCTTCCC TATCAGAAGA CCAGGTCCCC GGGCCATTCC TGGTGATGGT TCTCATTCAG
    TTTGGAACCA TGGTGGTGGA CCGTGCACTC TACCTCAGGA AGACTGTGCT TGGCAAGGTC
    ATTTTCCAGG TCATCCTCGT GTTTGGAATT CACTTCTGGA TGTTCTTCAT CCTACCCAGT
    GTGACTGAGA GGAAATTCAG CCAGAACCTG GTGGCACAGC TCTGGTACTT TGTGAAATGT
    GTGTACTTTG GGTTGTCTGC CTACCAAATC CGCTGTGGCT ACCCAACACG TGTGCTCGGA
    AACTTCCTCA CGAAGAGCTA CAATTATGTC AACCTCTTCT TGTTTCAAGG GTTTCGTCTC
    GTCCCATTTC TGACAGAGCT GCGGGCTGTG ATGGACTGGG TGTGGACAGA TACAACCTTG
    AGCCTCTCCA GCTGGATTTG TGTGGAGGAC ATTTACGCAC ACATTTTCAT CTTAAAGTGC
    TGGCGAGAGT CGGAAAAGAG ATACCCTCAA CCCAGGGGCC AGAAGAAGAA GAAAGCTGTG
    AAGTATGGCA TGGGCGGAAT GATCATTGTC CTGCTCATCT GTATCGTCTG GTTTCCTCTG
    CTCTTCATGT CTTTGATCAA GTCTGTCGCA GGGGTTATTA ACCAACCCCT GGATGTGTCC
    GTCACAATAA CCCTGGGAGG CTATCAGCCT ATCTTCACAA TGAGTGCCCA GCAAAGCCAG
    CTGAAAGTTA TGGATGGTAC AAAGTATAAC GAATTCATAA AGTCTTTCGG CCCCAACAGT
    GGCGCTATGC AATTTCTGGA GAATTATGAA AGGCAAGACA TAACAGTAGC GGAACTGGAA
    GGGAATTCGA ATTCTTTGTG GACCATCAGC CCTCCCAGTA AGCAGAAAAT GATACAGGAG
    CTCACCGACC CCAATAGTTG CTTCTCTGTT GTTTTTTCAT GGAGTATCCA GAGAAACATG
    ACTCTGGGTG CAAAGGCTGA AATAGCAACG GACAAGCTTT CTTTTCCTCT CACGGATGCG
    ACACGAAGTA GCATAGCCAA AATGATTGCT GGCAATGACA CAGAAAGTTC CAACACACCA
    GTGACAATAG AAAAGATCTA CCCATACTAT GTGAAGGCAC CCAGTGACTC AAACTCAAAG
    CCTATAAAAC AGCTTCTGTC AGAAAACAAT TTCATGAATA TCACCATCAT TTTGTTCAGA
    GACAATGTCA CAAAGTCCAA TAGTGAGTGG TGGGTTCTCA ACCTGACTGG AAGTAGGATA
    TTCAATCAGG GATCCCAAGC CTTGGAGCTG GTGGTCTTCA ATGACAAAGT CAGCCCCCCA
    AGTCTGGGCT TCTTGGCTGG CTATGGCATC ATGGGATTGT ATGCATCCGT TGTCCTTGTA
    ATTGGGAAGT TTGTTCGCGA ATTCTTCAGT GGGATTTCTC ACTCTATCAT GTTTGAAGAG
    CTTCCAAATG TGGACAGAAT TTTGAAGTTA TGCACAGATA TATTTCTCGT ACGGGAGACA
    GGGGAACTGG AGCTGGAGGA AGACCTCTAT GCCAAATTGA TATTCCTGTA CAGGTCGCCA
    GAAACCATGA TCAAGTGGAC TAGAGAAAAA ACAAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001063568.2
    CDS940..9198
    Translation

    Target ORF information:

    RefSeq Version XM_001063568.6
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_001063568.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTCCCCAT CTGCCTGGCA 
    GTAGCATGTG CATTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCG ACGATGCAAG GACACACAGG GCGGCTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCCTTT CTGCTCCTTC ACATCATTTT CCACATCACA
    CTGGCCAGCC TGGAAGCTCA GCACCGCATC GCACCCGCTT ACAACTGCTC AACTTGGGAA
    AAGACCTTCC GGCAGATCGG CTTCGAAAGC TTGAAGGGAG CTGATGCTGG CAATGGCATC
    AGGGTGTTTG TGCCTGATAT CGGGATGTTC ATTGCGAGCC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACGGAA GAAGTAGCCC AATTTAATTC CGAGTGCGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GACTCAGAAG AAGCGCTGAT TTATGAGGAG
    GACTTAGATG GGGAAGAAGG CATGGAAGGC GAGCTGGAAG AAAGCACAAA GTTAAAAATG
    TTTCGCAGGC TCGCCTCCGT CGCTTCCAAG CTTAAGGAGT TCATCGGCAA TATGATAACC
    ACGGCGGGCA AGGTGGTGGT GACCATCCTG TTGGGCTCCT CTGGCATGAT GCTGCCATCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT CTGGGTCTGT GCACCTGGTG GTCCTGGTGC
    CGAACTTTTG ACCCATTGCT GTTTGGCTGC CTCTGTGTCC TGTTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTTCAGTTCT TCCAAGAAGC AGTCCCACCC
    AATGACTACT ATGCAAGGTT GTTTGGAATC AAATCCGTTA TTCAAACAGA CTGCTCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTACAC GCTGGCCACG CTGATCCGCA TTTGGTTGCA AGAGCCTCTT
    GTGCAGGAGG AGATGTCGAA GGAAGAGGAG GGTGCACTGG ATGGCTGCTC CAATCAGAAC
    ACAGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    CTTTTATCCA TGACCCAAGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACTGTGAAC
    GGCAACCCTG TGGACTACCA CACCATCCAC CCAAGTCTGC CCATAGAGAA TGGCCCCGCC
    AAGACGGACT TGTATACTAC TCCTCAGTAC CGGTGGGAGC CCTCAGAGGA GTCCTCAGAA
    AAGAAAGAGG AGGAAGAGGA CAAGAGAGAA GACTCAGAAG GGGAAGGGAG CCAAGAGGAA
    AAACGGAGTG TGAGAATTCA TGCCATGGTC GCCGTGTTCC AGTTCATCAT GAAGCAGAGT
    TACATCTGTG CCCTCATCGC CATGATGGCA TGGAGCATCA CCTACCACAG CTGGCTGACA
    TTTGTGCTGT TGATCTGGTC GTGCACTCTC TGGATGATTC GCAACAGGAG GAAGTACGCC
    ATGATCAGCT CTCCGTTCAT GGTTGTCTAT GCCAATCTGC TGCTGGTACT GCAGTACATA
    TGGAGCTTTG AACTTCCAGA AATCAAGAAA GTCCCTGGAT TTCTAGAAAA GAAAGAGCCT
    GGAGAGCTTG CCTCAAAGAT CCTCTTCACC ATCACATTTT GGCTGCTCCT GAGGCAGCAT
    CTCACAGAGC AGAAGGCCCT TCAGGAAAAG GAAGCACTTC TGTCTGAAGT CAAAATTGGA
    AGTCAAGAAC TTGACGAAAA GGATGAGGAA CTCCAAGATA TACAAGTGGA AGGAGAGCCC
    ATAGAGAAGG AAGAAGAGGA GGAGGAAATA AAGGAAGACA GACATGAGGT AAAGAAGGAA
    GAGGAAGAGG AAGAGGAAGA TGACGATGAC CAGGACATCA TGAAGGTTCT GGGCAATCTG
    GTGGTGGCCC TCTTCATCAA GTATTGGATC TACGTCTGCG GGGGCATGTT CTTTTTTGTC
    AGCTTTGAGG GGAAAATCGT CATGTACAAA ATCATCTACA TGGTGCTGTT CCTGTTCTGT
    GTGGCCTTGT ACCAGGTGCA CTACGAGTGG TGGAGGAAGA TCCTGAAATA CTTCTGGATG
    TCGGTGGTCA TTTACACCAT GCTGGTGCTC ATCTTCATCT ACACTTACCA GTTTGAGAAC
    TTCCCAGGCC TGTGGCAAAA CATGACCGGG TTAAAGAAAG AGAAGCTTGA GGATCTGGGC
    CTGAAGCAGT TCACTGTGGC CGAACTGTTC ACACGAATAT TCATCCCCAC CTCCTTTCTG
    CTGGTCTGCA TCCTGCACCT CCACTATTTC CATGACCGGT TCCTGGAACT CACAGACCTC
    AAGTCCATCC CCAGCAAAGA AGACAACACC ATCTACAGGT TAGCCCACCC TGAAGGAAGC
    CTCCCAGACC TAGCCATTAT GAACATGACG GCCAGCCTCG ATAAGCCTGA GAGGCAGAAG
    TTGGCAGAGC CTGGGGAGGA GAGGACAGAA GAATGTGCCA AGAAGGCAGA GAAGGGTGAG
    GCTGGGAAAG ACAGTGATGA GTCTGAGGAG GAGGAAGGTG AGGAAGAGGA ATCCGAGGAG
    GAAGAATCCT CAGATCTCAG GAACAAGTGG CACCTCGTGA TTGACCGGCT CACTGTGCTC
    TTTCTAAAGT TCCTGGAGTA TTTCCACAAG TTGCAGGTGT TTATGTGGTG GATCTTGGAG
    CTGCACATCA TCAAAATCGT CTCATCGTAC ATTATCTGGG TGACTGTCAA AGAGGTGTCT
    CTGTTCAACT ATGTATTTTT GATTTCTTGG GCATTTGCTC TGCCATATGC CAAACTCCGC
    CGTGTGGCTT CCAGTGTCTG CACTGTCTGG ACGTGTGTGA TCATCGTCTG CAAAATGTTG
    TACCAGCTGC AAACCATTAA GCCTGAGAAC TTTTCTGTCA ACTGCTCCTT GCCCAATGAA
    AATCAAACCA ACATACCCCT TAATGAGTTG AACAAGTCTC TTCTCTACAG TGCCCCTGTT
    GATCCCACTG AGTGGGTTGG CCTGAGGAAG TCCTCCCCTT TACTCGTCTA CCTGAGGAAC
    AACCTCCTGA TGTTGGCGAT TTTGGCCTTC GAAGTCACTG TTTACCGACA TCAGGAGTAC
    TATCGAGGCC GAAACAACTT GACTGCTCCG GTGTCTAAAA CTATCTTTCA TGACATCACA
    AGGTTACATC TAGATGATGG GCTTATCAAT TGTGCCAAAT ATTTTGTAAA CTACTTCTTC
    TACAAGTTTG GCCTAGAGAC CTGTTTCCTA ATGTCAGTGA ACGTAATTGG ACAGCGAATG
    GACTTCTACG CCATGATTCA TGCCTGTTGG CTGATCGGTG TCTTGTACCG TCGCAGAAGA
    AAAGCCATTG CTGAGGTCTG GCCCAAGTAC TGCTGCTTCC TGGCTTGCAT CATCACCTTC
    CAGTACTTTG TCTGCATAGG CATCCCTCCT GCCCCCTGCC GAGATTACCC ATGGAGGTTC
    AAGGGTGCCA ACTTCAATGA CAACATCATC AAGTGGCTGT ACTTCCCAGA TTTCATCGTG
    CGGCCCAACC CTGTGTTTCT TGTCTATGAC TTCATGCTGC TCCTGTGTGC TTCCTTACAA
    CGACAGATTT TTGAGGACGA AAACAAGGCA GCTGTGCGCA TCATGGCCGG GGACAATGTC
    GAGATTTGCA TGAACCTTGA CGCGGCCTCA TTCAGCCAGC ATAACCCTGT ACCAGATTTC
    ATTCACTGCA GATCTTACTT GGACATGTCC AAAGTGATCA TCTTCAGCTA CCTCTTCTGG
    TTCGTCCTCA CCATCATCTT CATTACCGGA ACGACCAGGA TCAGCATCTT TTGCATGGGT
    TACCTGGTGG CCTGTTTCTA TTTCCTGCTC TTTGGAGGTG ATCTGCTCCT GAAACCCATC
    AAGAGCATCC TACGCTACTG GGACTGGCTG ATTGCATACA ACGTGTTTGT GATCACCATG
    AAAAACATAC TGTCAATAGG AGCCTGTGGA TACATCGGTG CACTGGTGAG AAACAGCTGC
    TGGCTGATCC AAGCGTTCAG CCTGGCCTGC ACGGTGAAAG GCTATAAAAT GCCTGAAGAT
    GACTCCAGAT GCAAGCTGCC CAGTGGGGAG GCAGGGATCA TCTGGGACAG CATCTGCTTT
    GCGTTTCTCC TGCTACAGAG GAGAGTCTTC ATGAGTTACT ATTTCCTGCA TGTCGTGGCT
    GACATTAAAG CATCCCAGAT CCTGGCATCC AGAGGGGCCG AACTTTTCCA GGCTACAATT
    GTGAAAGCTG TAAAGGCAAG AATTGAAGAA GAGAAAAAAT CCATGGACCA GCTGAAGAGA
    CAGATGGATC GCATCAAAGC CCGGCAGCAA AAGTACAAAA AGGGTAAGGA GAGGATGCTG
    AGCTTGACCC AGGAGTCAGG GGAAGGCCAG GACATCCAGA AAGTCTCAGA AGAAGACGAT
    GAAAGAGAAG CAGACAAACA GAAAGCCAAG GGCAAAAAAA AGCAGTGGTG GCGGCCTTGG
    GTTGATCATG CTTCCATGGT CAGGAGTGGA GATTATTATT TGTTTGAAAC GGATAGTGAA
    GAGGAGGAAG AAGAGGAACT CAAAAAGGAG GATGAAGAAC CACCACGGAA GTCTGCATTC
    CAGTTCGTCT ACCAAGCCTG GATTACCGAT CCTAAAACAG CACTCCGACA GAGGAGGAAA
    GAGAAGAAGA AAATGGCCAG AGAAGAGCAG AAGGGAAGAC GCAAAGGATC TGGGGAGGGT
    CCAGTGGAAT GGGAAGACAG AGAAGATGAG CCAATCAAAA AGAAATCTGA TGGACCAGAT
    AATATCATCA AGAGGATATT TAATATCCTG AAGTTCACCT GGGTCCTGTT CCTGGCCACA
    GTGGACAGTT TCACTACTTG GCTCAACTCC ATCTCAAGAG AGCATATCGA CATATCCACA
    GTTCTGCGGA TTGAAAGATG CATGCTGACC CGAGAGATAA AGAAGGGCAA CGTTCCGACA
    CGGGAAAGCA TCCACATGTA CTACCAGAAC CACATGATGA ACCTTTCCAG AGAATCCGGA
    CTGGACACAA TCGACGAGCA CTCTGGCGCT GGTTCCAGGG CCCAGGCAGC ACACAGGATG
    GATAGCTTAG ATTCCCATGA CAGTATCTCC AGCGAGCCCA CACAGTGCAC CATGCTGTAT
    TCACGCCAAG GAACTACGGA AACCATTGAG GAGGTAGAGG CTGAAGCTGA GGAGGAGGTG
    GTAGAGAGTC TGGAGCCTGA GCTCCATGAT GCCGAAGAGA AGGAATATGC AGCTGACTAT
    GAAGCAGGGG TAGAGGAGGT CAGCCTCACT CCAGATGAGG AGTTGCCTCA GTTCTCAACA
    GATGACGGTG ACGTGGAGGC CCCGCCCTCC TACAGCAAGG CTGTCAGCTT TGAGCACCTC
    TCGTTTGCAT CACAAGATGA CTCCGGGGGC AAGAACCACA TGGTGGTCAG CCCTGACGAC
    AGCCGCACAG ACAAGCTGGA GTCCAGTATC TTACCGCCCC TGACTCACGA GCTGACAGCC
    AGCGATCTGC TCATGAGCAA GATGTTCCGT GATGATGAGC TTGAAGAATC TGAGAAATTC
    TACGTGGACC AGCCCCGGTT CTTGCTGCTA TTCTATGCCA TGTATAACAC TCTGGTAGCC
    CGCTCGGAAA TGGTGTGCTA TTTCGTGATC ATCCTGAACC ACATGATCTC TGCCTCCATC
    ATTACTCTTC TGTTGCCCAT CCTGATCTTC CTCTGGGCCA TGCTGTCCGT CCCCAGGCCC
    AGCCGGCGCT TCTGGATGAT GGCCATCGTC TACACTGAGG TGGCGATTGT AGTGAAATAT
    TTCTTCCAGT TTGGCTTCTT TCCTTGGAAT AAGGACTTGG AAATTTACAA GGACAGGCCC
    TACTTTCCAC CAAACATCAT AGGGGTGGAA AAGAAAGAGG GCTATGTTCT TTACGACCTC
    ATTCAGCTGC TGGCTCTGTT CTTCCATCGA TCCATTCTGA AGTGTCATGG CCTATGGGAC
    GAGGATGACA TTGTTGACAG TAACACAGAC AAGGAAGGAT CTGATGATGA GCTGTCCCTC
    GGTCAGGGCA GAAGAGGCTC CTCAGAGTCA CTGAAGTCCA TCAACTTGGC TGCCTCTGTA
    GAGTCCGTCC ATGTGACCTT CCCAGAGCAG CCAGCAGCCA TCCGGAGAAA ACGCTCCTGC
    AGCAGCTCGC AGATCTCCCC ACGGTCCAGC TTCTCTTCAA ATAGGTCCAA AAGAGGCAGC
    ACAAGCACCC GAAACAGCAG CCAAAAAGGA AGCAGTGTTT TGAGCATTAA ACAAAAAAGC
    AAAAGAGAAC TTTACATGGA AAAGCTTCAA GAACATTTAA TCAAAGCAAA GGCCTTTACC
    ATAAAGAAAA CTCTACAAAT CTATGTACCC ATTCGGCAAT TCTTCTATGA CCTCATCCAC
    CCAGACTACA GTGCTGTGAC TGACGTGTAT GTGCTGATGT TCCTGGCCGA CACCGTGGAC
    TTCATCATCA TCGTCTTTGG CTTCTGGGCT TTTGGGAAAC ACTCGGCAGC TGCAGATATC
    ACCTCTTCCC TATCAGAAGA CCAGGTCCCC GGGCCATTCC TGGTGATGGT TCTCATTCAG
    TTTGGAACCA TGGTGGTGGA CCGTGCACTC TACCTCAGGA AGACTGTGCT TGGCAAGGTC
    ATTTTCCAGG TCATCCTCGT GTTTGGAATT CACTTCTGGA TGTTCTTCAT CCTACCCAGT
    GTGACTGAGA GGAAATTCAG CCAGAACCTG GTGGCACAGC TCTGGTACTT TGTGAAATGT
    GTGTACTTTG GGTTGTCTGC CTACCAAATC CGCTGTGGCT ACCCAACACG TGTGCTCGGA
    AACTTCCTCA CGAAGAGCTA CAATTATGTC AACCTCTTCT TGTTTCAAGG GTTTCGTCTC
    GTCCCATTTC TGACAGAGCT GCGGGCTGTG ATGGACTGGG TGTGGACAGA TACAACCTTG
    AGCCTCTCCA GCTGGATTTG TGTGGAGGAC ATTTACGCAC ACATTTTCAT CTTAAAGTGC
    TGGCGAGAGT CGGAAAAGAG ATACCCTCAA CCCAGGGGCC AGAAGAAGAA GAAAGCTGTG
    AAGTATGGCA TGGGCGGAAT GATCATTGTC CTGCTCATCT GTATCGTCTG GTTTCCTCTG
    CTCTTCATGT CTTTGATCAA GTCTGTCGCA GGGGTTATTA ACCAACCCCT GGATGTGTCC
    GTCACAATAA CCCTGGGAGG CTATCAGCCT ATCTTCACAA TGAGTGCCCA GCAAAGCCAG
    CTGAAAGTTA TGGATGGTAC AAAGTATAAC GAATTCATAA AGTCTTTCGG CCCCAACAGT
    GGCGCTATGC AATTTCTGGA GAATTATGAA AGGCAAGACA TAACAGTAGC GGAACTGGAA
    GGGAATTCGA ATTCTTTGTG GACCATCAGC CCTCCCAGTA AGCAGAAAAT GATACAGGAG
    CTCACCGACC CCAATAGTTG CTTCTCTGTT GTTTTTTCAT GGAGTATCCA GAGAAACATG
    ACTCTGGGTG CAAAGGCTGA AATAGCAACG GACAAGCTTT CTTTTCCTCT CACGGATGCG
    ACACGAAGTA GCATAGCCAA AATGATTGCT GGCAATGACA CAGAAAGTTC CAACACACCA
    GTGACAATAG AAAAGATCTA CCCATACTAT GTGAAGGCAC CCAGTGACTC AAACTCAAAG
    CCTATAAAAC AGCTTCTGTC AGAAAACAAT TTCATGAATA TCACCATCAT TTTGTTCAGA
    GACAATGTCA CAAAGTCCAA TAGTGAGTGG TGGGTTCTCA ACCTGACTGG AAGTAGGATA
    TTCAATCAGG GATCCCAAGC CTTGGAGCTG GTGGTCTTCA ATGACAAAGT CAGCCCCCCA
    AGTCTGGGCT TCTTGGCTGG CTATGGCATC ATGGGATTGT ATGCATCCGT TGTCCTTGTA
    ATTGGGAAGT TTGTTCGCGA ATTCTTCAGT GGGATTTCTC ACTCTATCAT GTTTGAAGAG
    CTTCCAAATG TGGACAGAAT TTTGAAGTTA TGCACAGATA TATTTCTCGT ACGGGAGACA
    GGGGAACTGG AGCTGGAGGA AGACCTCTAT GCCAAATTGA TATTCCTGTA CAGGTCGCCA
    GAAACCATGA TCAAGTGGAC TAGAGAAAAA ACAAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa54716
    Clone ID Related Accession (Same CDS sequence) XM_017601114.1 , XM_017587875.1
    Accession Version XM_017601114.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8601bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product piezo-type mechanosensitive ion channel component 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005117.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTCCCCAT CTGCCTGGCA 
    GTAGCATGTG CATTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCG ACGATGCAAG GACACACAGG GCGGCTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCCTTT CTGCTCCTTC ACATCATTTT CCACATCACA
    CTGGCCAGCC TGGAAGCTCA GCACCGCATC GCACCCGCTT ACAACTGCTC AACTTGGGAA
    AAGACCTTCC GGCAGATCGG CTTCGAAAGC TTGAAGGGAG CTGATGCTGG CAATGGCATC
    AGGGTGTTTG TGCCTGATAT CGGGATGTTC ATTGCGAGCC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACGGAA GAAGTAGCCC AATTTAATTC CGAGTGCGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GACTCAGAAG AAGCGCTGAT TTATGAGGAG
    GACTTAGATG GGGAAGAAGG CATGGAAGGC GAGCTGGAAG AAAGCACAAA GTTAAAAATG
    TTTCGCAGGC TCGCCTCCGT CGCTTCCAAG CTTAAGGAGT TCATCGGCAA TATGATAACC
    ACGGCGGGCA AGGTGGTGGT GACCATCCTG TTGGGCTCCT CTGGCATGAT GCTGCCATCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT CTGGGTCTGT GCACCTGGTG GTCCTGGTGC
    CGAACTTTTG ACCCATTGCT GTTTGGCTGC CTCTGTGTCC TGTTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTTCAGTTCT TCCAAGAAGC AGTCCCACCC
    AATGACTACT ATGCAAGGTT GTTTGGAATC AAATCCGTTA TTCAAACAGA CTGCTCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTACAC GCTGGCCACG CTGATCCGCA TTTGGTTGCA AGAGCCTCTT
    GTGCAGGAGG AGATGTCGAA GGAAGAGGAG GGTGCACTGG ATGGCTGCTC CAATCAGAAC
    ACAGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    CTTTTATCCA TGACCCAAGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACTGTGAAC
    GGCAACCCTG TGGACTACCA CACCATCCAC CCAAGTCTGC CCATAGAGAA TGGCCCCGCC
    AAGACGGACT TGTATACTAC TCCTCAGTAC CGGTGGGAGC CCTCAGAGGA GTCCTCAGAA
    AAGAAAGAGG AGGAAGAGGA CAAGAGAGAA GACTCAGAAG GGGAAGGGAG CCAAGAGGAA
    AAACGGAGTG TGAGAATTCA TGCCATGGTC GCCGTGTTCC AGTTCATCAT GAAGCAGAGT
    TACATCTGTG CCCTCATCGC CATGATGGCA TGGAGCATCA CCTACCACAG CTGGCTGACA
    TTTGTGCTGT TGATCTGGTC GTGCACTCTC TGGATGATTC GCAACAGGAG GAAGTACGCC
    ATGATCAGCT CTCCGTTCAT GGTTGTCTAT GCCAATCTGC TGCTGGTACT GCAGTACATA
    TGGAGCTTTG AACTTCCAGA AATCAAGAAA GTCCCTGGAT TTCTAGAAAA GAAAGAGCCT
    GGAGAGCTTG CCTCAAAGAT CCTCTTCACC ATCACATTTT GGCTGCTCCT GAGGCAGCAT
    CTCACAGAGC AGAAGGCCCT TCAGGAAAAG GAAGCACTTC TGTCTGAAGT CAAAATTGGA
    AGTCAAGAAC TTGACGAAAA GGATGAGGAA CTCCAAGATA TACAAGTGGA AGGAGAGCCC
    ATAGAGAAGG AAGAAGAGGA GGAGGAAATA AAGGAAGACA GACATGAGGT AAAGAAGGAA
    GAGGAAGAGG AAGAGGAAGA TGACGATGAC CAGGACATCA TGAAGGTTCT GGGCAATCTG
    GTGGTGGCCC TCTTCATCAA GTATTGGATC TACGTCTGCG GGGGCATGTT CTTTTTTGTC
    AGCTTTGAGG GGAAAATCGT CATGTACAAA ATCATCTACA TGGTGCTGTT CCTGTTCTGT
    GTGGCCTTGT ACCAGGTGCA CTACGAGTGG TGGAGGAAGA TCCTGAAATA CTTCTGGATG
    TCGGTGGTCA TTTACACCAT GCTGGTGCTC ATCTTCATCT ACACTTACCA GTTTGAGAAC
    TTCCCAGGCC TGTGGCAAAA CATGACCGGG TTAAAGAAAG AGAAGCTTGA GGATCTGGGC
    CTGAAGCAGT TCACTGTGGC CGAACTGTTC ACACGAATAT TCATCCCCAC CTCCTTTCTG
    CTGGTCTGCA TCCTGCACCT CCACTATTTC CATGACCGGT TCCTGGAACT CACAGACCTC
    AAGTCCATCC CCAGCAAAGA AGACAACACC ATCTACAGCC ATGCCAAGGT CAATGGTCGC
    GTGTACCTAA TAATAAATAG CATCAAGAAA AAATTACCAA TCCACCAGAA TGAGTTAGCC
    CACCCTGAAG GAAGCCTCCC AGACCTAGCC ATTATGAACA TGACGGCCAG CCTCGATAAG
    CCTGAGAGGC AGAAGTTGGC AGAGCCTGGG GAGGAGAGGA CAGAAGAATG TGCCAAGAAG
    GCAGAGAAGG GTGAGGCTGG GAAAGACAGT GATGAGTCTG AGGAGGAGGA AGGTGAGGAA
    GAGGAATCCG AGGAGGAAGA ATCCTCAGAT CTCAGGAACA AGTGGCACCT CGTGATTGAC
    CGGCTCACTG TGCTCTTTCT AAAGTTCCTG GAGTATTTCC ACAAGTTGCA GGTGTTTATG
    TGGTGGATCT TGGAGCTGCA CATCATCAAA ATCGTCTCAT CGTACATTAT CTGGGTGACT
    GTCAAAGAGG TGTCTCTGTT CAACTATGTA TTTTTGATTT CTTGGGCATT TGCTCTGCCA
    TATGCCAAAC TCCGCCGTGT GGCTTCCAGT GTCTGCACTG TCTGGACGTG TGTGATCATC
    GTCTGCAAAA TGTTGTACCA GCTGCAAACC ATTAAGCCTG AGAACTTTTC TGTCAACTGC
    TCCTTGCCCA ATGAAAATCA AACCAACATA CCCCTTAATG AGTTGAACAA GTCTCTTCTC
    TACAGTGCCC CTGTTGATCC CACTGAGTGG GTTGGCCTGA GGAAGTCCTC CCCTTTACTC
    GTCTACCTGA GGAACAACCT CCTGATGTTG GCGATTTTGG CCTTCGAAGT CACTGTTTAC
    CGACATCAGG AGTACTATCG AGGCCGAAAC AACTTGACTG CTCCGGTGTC TAAAACTATC
    TTTCATGACA TCACAAGGTT ACATCTAGAT GATGGGCTTA TCAATTGTGC CAAATATTTT
    GTAAACTACT TCTTCTACAA GTTTGGCCTA GAGACCTGTT TCCTAATGTC AGTGAACGTA
    ATTGGACAGC GAATGGACTT CTACGCCATG ATTCATGCCT GTTGGCTGAT CGGTGTCTTG
    TACCGTCGCA GAAGAAAAGC CATTGCTGAG GTCTGGCCCA AGTACTGCTG CTTCCTGGCT
    TGCATCATCA CCTTCCAGTA CTTTGTCTGC ATAGGCATCC CTCCTGCCCC CTGCCGAGAT
    TACCCATGGA GGTTCAAGGG TGCCAACTTC AATGACAACA TCATCAAGTG GCTGTACTTC
    CCAGATTTCA TCGTGCGGCC CAACCCTGTG TTTCTTGTCT ATGACTTCAT GCTGCTCCTG
    TGTGCTTCCT TACAACGACA GATTTTTGAG GACGAAAACA AGGCAGCTGT GCGCATCATG
    GCCGGGGACA ATGTCGAGAT TTGCATGAAC CTTGACGCGG CCTCATTCAG CCAGCATAAC
    CCTGTACCAG ATTTCATTCA CTGCAGATCT TACTTGGACA TGTCCAAAGT GATCATCTTC
    AGCTACCTCT TCTGGTTCGT CCTCACCATC ATCTTCATTA CCGGAACGAC CAGGATCAGC
    ATCTTTTGCA TGGGTTACCT GGTGGCCTGT TTCTATTTCC TGCTCTTTGG AGGTGATCTG
    CTCCTGAAAC CCATCAAGAG CATCCTACGC TACTGGGACT GGCTGATTGC ATACAACGTG
    TTTGTGATCA CCATGAAAAA CATACTGTCA ATAGGAGCCT GTGGATACAT CGGTGCACTG
    GTGAGAAACA GCTGCTGGCT GATCCAAGCG TTCAGCCTGG CCTGCACGGT GAAAGGCTAT
    AAAATGCCTG AAGATGACTC CAGATGCAAG CTGCCCAGTG GGGAGGCAGG GATCATCTGG
    GACAGCATCT GCTTTGCGTT TCTCCTGCTA CAGAGGAGAG TCTTCATGAG TTACTATTTC
    CTGCATGTCG TGGCTGACAT TAAAGCATCC CAGATCCTGG CATCCAGAGG GGCCGAACTT
    TTCCAGGCTA CAATTGTGAA AGCTGTAAAG GCAAGAATTG AAGAAGAGAA AAAATCCATG
    GACCAGCTGA AGAGACAGAT GGATCGCATC AAAGCCCGGC AGCAAAAGTA CAAAAAGGGT
    AAGGAGAGGA TGCTGAGCTT GACCCAGGAG TCAGGGGAAG GCCAGGACAT CCAGAAAGTC
    TCAGAAGAAG ACGATGAAAG AGAAGCAGAC AAACAGAAAG CCAAGGGCAA AAAAAAGCAG
    TGGTGGCGGC CTTGGGTTGA TCATGCTTCC ATGGTCAGGA GTGGAGATTA TTATTTGTTT
    GAAACGGATA GTGAAGAGGA GGAAGAAGAG GAACTCAAAA AGGAGGATGA AGAACCACCA
    CGGAAGTCTG CATTCCAGAG AGCTATTGGG AAGTTTGCCT CAGCCATTTT AGCCTTGCCA
    AAGTCTGTCA TTAAACTTCC CAAAACTATT CTTCAGTATT TAATCAGAGC TGCAAAGTTC
    GTCTACCAAG CCTGGATTAC CGATCCTAAA ACAGCACTCC GACAGAGGAG GAAAGAGAAG
    AAGAAAATGG CCAGAGAAGA GCAGAAGGGA AGACGCAAAG GATCTGGGGA GGGTCCAGTG
    GAATGGGAAG ACAGAGAAGA TGAGCCAATC AAAAAGAAAT CTGATGGACC AGATAATATC
    ATCAAGAGGA TATTTAATAT CCTGAAGTTC ACCTGGGTCC TGTTCCTGGC CACAGTGGAC
    AGTTTCACTA CTTGGCTCAA CTCCATCTCA AGAGAGCATA TCGACATATC CACAGTTCTG
    CGGATTGAAA GATGCATGCT GACCCGAGAG ATAAAGAAGG GCAACGTTCC GACACGGGAA
    AGCATCCACA TGTACTACCA GAACCACATG ATGAACCTTT CCAGAGAATC CGGACTGGAC
    ACAATCGACG AGCACTCTGG CGCTGGTTCC AGGGCCCAGG CAGCACACAG GATGGATAGC
    TTAGATTCCC ATGACAGTAT CTCCAGCTGT TACACTGAAG CAACCCTGCT GATCTCAAGG
    CAGTCCACCC TTGATGATTT AGATGGACAA GACCCTGTTC CTAAAACAAG CGAGCGTGCT
    CGGCCTAGGC TCCGTAAAGT GTTCAGCATG GACATGTCCT CCTCATCAGC TGACAGTGGC
    AGTGTAGCAT CAAGCGAGCC CACACAGTGC ACCATGCTGT ATTCACGCCA AGGAACTACG
    GAAACCATTG AGGAGGTAGA GGCTGAAGCT GAGGAGGAGG TGGTAGAGAG TCTGGAGCCT
    GAGCTCCATG ATGCCGAAGA GAAGGAATAT GCAGCTGACT ATGAAGCAGG GGTAGAGGAG
    GTCAGCCTCA CTCCAGATGA GGAGTTGCCT CAGTTCTCAA CAGATGACGG TGACGTGGAG
    GCCCCGCCCT CCTACAGCAA GGCTGTCAGC TTTGAGCACC TCTCGTTTGC ATCACAAGAT
    GACTCCGGGG GCAAGAACCA CATGGTGGTC AGCCCTGACG ACAGCCGCAC AGACAAGCTG
    GAGTCCAGTA TCTTACCGCC CCTGACTCAC GAGCTGACAG CCAGCGATCT GCTCATGAGC
    AAGATGTTCC GTGATGATGA GCTTGAAGAA TCTGAGAAAT TCTACGTGGA CCAGCCCCGG
    TTCTTGCTGC TATTCTATGC CATGTATAAC ACTCTGGTAG CCCGCTCGGA AATGGTGTGC
    TATTTCGTGA TCATCCTGAA CCACATGATC TCTGCCTCCA TCATTACTCT TCTGTTGCCC
    ATCCTGATCT TCCTCTGGGC CATGCTGTCC GTCCCCAGGC CCAGCCGGCG CTTCTGGATG
    ATGGCCATCG TCTACACTGA GGTGGCGATT GTAGTGAAAT ATTTCTTCCA GTTTGGCTTC
    TTTCCTTGGA ATAAGGACTT GGAAATTTAC AAGGACAGGC CCTACTTTCC ACCAAACATC
    ATAGGGGTGG AAAAGAAAGA GGGCTATGTT CTTTACGACC TCATTCAGCT GCTGGCTCTG
    TTCTTCCATC GATCCATTCT GAAGTGTCAT GGCCTATGGG ACGAGGATGA CATTGTTGAC
    AGTAACACAG ACAAGGAAGG ATCTGATGAT GAGCTGTCCC TCGGTCAGGG CAGAAGAGGC
    TCCTCAGAGT CACTGAAGTC CATCAACTTG GCTGCCTCTG TAGAGTCCGT CCATGTGACC
    TTCCCAGAGC AGCCAGCAGC CATCCGGAGA AAACGCTCCT GCAGCAGCTC GCAGATCTCC
    CCACGGTCCA GCTTCTCTTC AAATAGGTCC AAAAGAGGCA GCACAAGCAC CCGAAACAGC
    AGCCAAAAAG GAAGCAGTGT TTTGAGCATT AAACAAAAAA GCAAAAGAGA ACTTTACATG
    GAAAAGCTTC AAGAACATTT AATCAAAGCA AAGGCCTTTA CCATAAAGAA AACTCTACAA
    ATCTATGTAC CCATTCGGCA ATTCTTCTAT GACCTCATCC ACCCAGACTA CAGTGCTGTG
    ACTGACGTGT ATGTGCTGAT GTTCCTGGCC GACACCGTGG ACTTCATCAT CATCGTCTTT
    GGCTTCTGGG CTTTTGGGAA ACACTCGGCA GCTGCAGATA TCACCTCTTC CCTATCAGAA
    GACCAGGTCC CCGGGCCATT CCTGGTGATG GTTCTCATTC AGTTTGGAAC CATGGTGGTG
    GACCGTGCAC TCTACCTCAG GAAGACTGTG CTTGGCAAGG TCATTTTCCA GGTCATCCTC
    GTGTTTGGAA TTCACTTCTG GATGTTCTTC ATCCTACCCA GTGTGACTGA GAGGAAATTC
    AGCCAGAACC TGGTGGCACA GCTCTGGTAC TTTGTGAAAT GTGTGTACTT TGGGTTGTCT
    GCCTACCAAA TCCGCTGTGG CTACCCAACA CGTGTGCTCG GAAACTTCCT CACGAAGAGC
    TACAATTATG TCAACCTCTT CTTGTTTCAA GGGTTTCGTC TCGTCCCATT TCTGACAGAG
    CTGCGGGCTG TGATGGACTG GGTGTGGACA GATACAACCT TGAGCCTCTC CAGCTGGATT
    TGTGTGGAGG ACATTTACGC ACACATTTTC ATCTTAAAGT GCTGGCGAGA GTCGGAAAAG
    AGATACCCTC AACCCAGGGG CCAGAAGAAG AAGAAAGCTG TGAAGTATGG CATGGGCGGA
    ATGATCATTG TCCTGCTCAT CTGTATCGTC TGGTTTCCTC TGCTCTTCAT GTCTTTGATC
    AAGTCTGTCG CAGGGGTTAT TAACCAACCC CTGGATGTGT CCGTCACAAT AACCCTGGGA
    GGCTATCAGC CTATCTTCAC AATGAGTGCC CAGCAAAGCC AGCTGAAAGT TATGGATGGT
    ACAAAGTATA ACGAATTCAT AAAGTCTTTC GGCCCCAACA GTGGCGCTAT GCAATTTCTG
    GAGAATTATG AAAGGCAAGA CATAACAGTA GCGGAACTGG AAGGGAATTC GAATTCTTTG
    TGGACCATCA GCCCTCCCAG TAAGCAGAAA ATGATACAGG AGCTCACCGA CCCCAATAGT
    TGCTTCTCTG TTGTTTTTTC ATGGAGTATC CAGAGAAACA TGACTCTGGG TGCAAAGGCT
    GAAATAGCAA CGGACAAGCT TTCTTTTCCT CTCACGGATG CGACACGAAG TAGCATAGCC
    AAAATGATTG CTGGCAATGA CACAGAAAGT TCCAACACAC CAGTGACAAT AGAAAAGATC
    TACCCATACT ATGTGAAGGC ACCCAGTGAC TCAAACTCAA AGCCTATAAA ACAGCTTCTG
    TCAGAAAACA ATTTCATGAA TATCACCATC ATTTTGTTCA GAGACAATGT CACAAAGTCC
    AATAGTGAGT GGTGGGTTCT CAACCTGACT GGAAGTAGGA TATTCAATCA GGGATCCCAA
    GCCTTGGAGC TGGTGGTCTT CAATGACAAA GTCAGCCCCC CAAGTCTGGG CTTCTTGGCT
    GGCTATGGCA TCATGGGATT GTATGCATCC GTTGTCCTTG TAATTGGGAA GTTTGTTCGC
    GAATTCTTCA GTGGGATTTC TCACTCTATC ATGTTTGAAG AGCTTCCAAA TGTGGACAGA
    ATTTTGAAGT TATGCACAGA TATATTTCTC GTACGGGAGA CAGGGGAACT GGAGCTGGAG
    GAAGACCTCT ATGCCAAATT GATATTCCTG TACAGGTCGC CAGAAACCAT GATCAAGTGG
    ACTAGAGAAA AAACAAATTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017456603.1
    CDS940..9540
    Translation

    Target ORF information:

    RefSeq Version XM_017601114.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017601114.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTCCCCAT CTGCCTGGCA 
    GTAGCATGTG CATTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCG ACGATGCAAG GACACACAGG GCGGCTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCCTTT CTGCTCCTTC ACATCATTTT CCACATCACA
    CTGGCCAGCC TGGAAGCTCA GCACCGCATC GCACCCGCTT ACAACTGCTC AACTTGGGAA
    AAGACCTTCC GGCAGATCGG CTTCGAAAGC TTGAAGGGAG CTGATGCTGG CAATGGCATC
    AGGGTGTTTG TGCCTGATAT CGGGATGTTC ATTGCGAGCC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACGGAA GAAGTAGCCC AATTTAATTC CGAGTGCGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GACTCAGAAG AAGCGCTGAT TTATGAGGAG
    GACTTAGATG GGGAAGAAGG CATGGAAGGC GAGCTGGAAG AAAGCACAAA GTTAAAAATG
    TTTCGCAGGC TCGCCTCCGT CGCTTCCAAG CTTAAGGAGT TCATCGGCAA TATGATAACC
    ACGGCGGGCA AGGTGGTGGT GACCATCCTG TTGGGCTCCT CTGGCATGAT GCTGCCATCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT CTGGGTCTGT GCACCTGGTG GTCCTGGTGC
    CGAACTTTTG ACCCATTGCT GTTTGGCTGC CTCTGTGTCC TGTTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTTCAGTTCT TCCAAGAAGC AGTCCCACCC
    AATGACTACT ATGCAAGGTT GTTTGGAATC AAATCCGTTA TTCAAACAGA CTGCTCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTACAC GCTGGCCACG CTGATCCGCA TTTGGTTGCA AGAGCCTCTT
    GTGCAGGAGG AGATGTCGAA GGAAGAGGAG GGTGCACTGG ATGGCTGCTC CAATCAGAAC
    ACAGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    CTTTTATCCA TGACCCAAGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACTGTGAAC
    GGCAACCCTG TGGACTACCA CACCATCCAC CCAAGTCTGC CCATAGAGAA TGGCCCCGCC
    AAGACGGACT TGTATACTAC TCCTCAGTAC CGGTGGGAGC CCTCAGAGGA GTCCTCAGAA
    AAGAAAGAGG AGGAAGAGGA CAAGAGAGAA GACTCAGAAG GGGAAGGGAG CCAAGAGGAA
    AAACGGAGTG TGAGAATTCA TGCCATGGTC GCCGTGTTCC AGTTCATCAT GAAGCAGAGT
    TACATCTGTG CCCTCATCGC CATGATGGCA TGGAGCATCA CCTACCACAG CTGGCTGACA
    TTTGTGCTGT TGATCTGGTC GTGCACTCTC TGGATGATTC GCAACAGGAG GAAGTACGCC
    ATGATCAGCT CTCCGTTCAT GGTTGTCTAT GCCAATCTGC TGCTGGTACT GCAGTACATA
    TGGAGCTTTG AACTTCCAGA AATCAAGAAA GTCCCTGGAT TTCTAGAAAA GAAAGAGCCT
    GGAGAGCTTG CCTCAAAGAT CCTCTTCACC ATCACATTTT GGCTGCTCCT GAGGCAGCAT
    CTCACAGAGC AGAAGGCCCT TCAGGAAAAG GAAGCACTTC TGTCTGAAGT CAAAATTGGA
    AGTCAAGAAC TTGACGAAAA GGATGAGGAA CTCCAAGATA TACAAGTGGA AGGAGAGCCC
    ATAGAGAAGG AAGAAGAGGA GGAGGAAATA AAGGAAGACA GACATGAGGT AAAGAAGGAA
    GAGGAAGAGG AAGAGGAAGA TGACGATGAC CAGGACATCA TGAAGGTTCT GGGCAATCTG
    GTGGTGGCCC TCTTCATCAA GTATTGGATC TACGTCTGCG GGGGCATGTT CTTTTTTGTC
    AGCTTTGAGG GGAAAATCGT CATGTACAAA ATCATCTACA TGGTGCTGTT CCTGTTCTGT
    GTGGCCTTGT ACCAGGTGCA CTACGAGTGG TGGAGGAAGA TCCTGAAATA CTTCTGGATG
    TCGGTGGTCA TTTACACCAT GCTGGTGCTC ATCTTCATCT ACACTTACCA GTTTGAGAAC
    TTCCCAGGCC TGTGGCAAAA CATGACCGGG TTAAAGAAAG AGAAGCTTGA GGATCTGGGC
    CTGAAGCAGT TCACTGTGGC CGAACTGTTC ACACGAATAT TCATCCCCAC CTCCTTTCTG
    CTGGTCTGCA TCCTGCACCT CCACTATTTC CATGACCGGT TCCTGGAACT CACAGACCTC
    AAGTCCATCC CCAGCAAAGA AGACAACACC ATCTACAGCC ATGCCAAGGT CAATGGTCGC
    GTGTACCTAA TAATAAATAG CATCAAGAAA AAATTACCAA TCCACCAGAA TGAGTTAGCC
    CACCCTGAAG GAAGCCTCCC AGACCTAGCC ATTATGAACA TGACGGCCAG CCTCGATAAG
    CCTGAGAGGC AGAAGTTGGC AGAGCCTGGG GAGGAGAGGA CAGAAGAATG TGCCAAGAAG
    GCAGAGAAGG GTGAGGCTGG GAAAGACAGT GATGAGTCTG AGGAGGAGGA AGGTGAGGAA
    GAGGAATCCG AGGAGGAAGA ATCCTCAGAT CTCAGGAACA AGTGGCACCT CGTGATTGAC
    CGGCTCACTG TGCTCTTTCT AAAGTTCCTG GAGTATTTCC ACAAGTTGCA GGTGTTTATG
    TGGTGGATCT TGGAGCTGCA CATCATCAAA ATCGTCTCAT CGTACATTAT CTGGGTGACT
    GTCAAAGAGG TGTCTCTGTT CAACTATGTA TTTTTGATTT CTTGGGCATT TGCTCTGCCA
    TATGCCAAAC TCCGCCGTGT GGCTTCCAGT GTCTGCACTG TCTGGACGTG TGTGATCATC
    GTCTGCAAAA TGTTGTACCA GCTGCAAACC ATTAAGCCTG AGAACTTTTC TGTCAACTGC
    TCCTTGCCCA ATGAAAATCA AACCAACATA CCCCTTAATG AGTTGAACAA GTCTCTTCTC
    TACAGTGCCC CTGTTGATCC CACTGAGTGG GTTGGCCTGA GGAAGTCCTC CCCTTTACTC
    GTCTACCTGA GGAACAACCT CCTGATGTTG GCGATTTTGG CCTTCGAAGT CACTGTTTAC
    CGACATCAGG AGTACTATCG AGGCCGAAAC AACTTGACTG CTCCGGTGTC TAAAACTATC
    TTTCATGACA TCACAAGGTT ACATCTAGAT GATGGGCTTA TCAATTGTGC CAAATATTTT
    GTAAACTACT TCTTCTACAA GTTTGGCCTA GAGACCTGTT TCCTAATGTC AGTGAACGTA
    ATTGGACAGC GAATGGACTT CTACGCCATG ATTCATGCCT GTTGGCTGAT CGGTGTCTTG
    TACCGTCGCA GAAGAAAAGC CATTGCTGAG GTCTGGCCCA AGTACTGCTG CTTCCTGGCT
    TGCATCATCA CCTTCCAGTA CTTTGTCTGC ATAGGCATCC CTCCTGCCCC CTGCCGAGAT
    TACCCATGGA GGTTCAAGGG TGCCAACTTC AATGACAACA TCATCAAGTG GCTGTACTTC
    CCAGATTTCA TCGTGCGGCC CAACCCTGTG TTTCTTGTCT ATGACTTCAT GCTGCTCCTG
    TGTGCTTCCT TACAACGACA GATTTTTGAG GACGAAAACA AGGCAGCTGT GCGCATCATG
    GCCGGGGACA ATGTCGAGAT TTGCATGAAC CTTGACGCGG CCTCATTCAG CCAGCATAAC
    CCTGTACCAG ATTTCATTCA CTGCAGATCT TACTTGGACA TGTCCAAAGT GATCATCTTC
    AGCTACCTCT TCTGGTTCGT CCTCACCATC ATCTTCATTA CCGGAACGAC CAGGATCAGC
    ATCTTTTGCA TGGGTTACCT GGTGGCCTGT TTCTATTTCC TGCTCTTTGG AGGTGATCTG
    CTCCTGAAAC CCATCAAGAG CATCCTACGC TACTGGGACT GGCTGATTGC ATACAACGTG
    TTTGTGATCA CCATGAAAAA CATACTGTCA ATAGGAGCCT GTGGATACAT CGGTGCACTG
    GTGAGAAACA GCTGCTGGCT GATCCAAGCG TTCAGCCTGG CCTGCACGGT GAAAGGCTAT
    AAAATGCCTG AAGATGACTC CAGATGCAAG CTGCCCAGTG GGGAGGCAGG GATCATCTGG
    GACAGCATCT GCTTTGCGTT TCTCCTGCTA CAGAGGAGAG TCTTCATGAG TTACTATTTC
    CTGCATGTCG TGGCTGACAT TAAAGCATCC CAGATCCTGG CATCCAGAGG GGCCGAACTT
    TTCCAGGCTA CAATTGTGAA AGCTGTAAAG GCAAGAATTG AAGAAGAGAA AAAATCCATG
    GACCAGCTGA AGAGACAGAT GGATCGCATC AAAGCCCGGC AGCAAAAGTA CAAAAAGGGT
    AAGGAGAGGA TGCTGAGCTT GACCCAGGAG TCAGGGGAAG GCCAGGACAT CCAGAAAGTC
    TCAGAAGAAG ACGATGAAAG AGAAGCAGAC AAACAGAAAG CCAAGGGCAA AAAAAAGCAG
    TGGTGGCGGC CTTGGGTTGA TCATGCTTCC ATGGTCAGGA GTGGAGATTA TTATTTGTTT
    GAAACGGATA GTGAAGAGGA GGAAGAAGAG GAACTCAAAA AGGAGGATGA AGAACCACCA
    CGGAAGTCTG CATTCCAGAG AGCTATTGGG AAGTTTGCCT CAGCCATTTT AGCCTTGCCA
    AAGTCTGTCA TTAAACTTCC CAAAACTATT CTTCAGTATT TAATCAGAGC TGCAAAGTTC
    GTCTACCAAG CCTGGATTAC CGATCCTAAA ACAGCACTCC GACAGAGGAG GAAAGAGAAG
    AAGAAAATGG CCAGAGAAGA GCAGAAGGGA AGACGCAAAG GATCTGGGGA GGGTCCAGTG
    GAATGGGAAG ACAGAGAAGA TGAGCCAATC AAAAAGAAAT CTGATGGACC AGATAATATC
    ATCAAGAGGA TATTTAATAT CCTGAAGTTC ACCTGGGTCC TGTTCCTGGC CACAGTGGAC
    AGTTTCACTA CTTGGCTCAA CTCCATCTCA AGAGAGCATA TCGACATATC CACAGTTCTG
    CGGATTGAAA GATGCATGCT GACCCGAGAG ATAAAGAAGG GCAACGTTCC GACACGGGAA
    AGCATCCACA TGTACTACCA GAACCACATG ATGAACCTTT CCAGAGAATC CGGACTGGAC
    ACAATCGACG AGCACTCTGG CGCTGGTTCC AGGGCCCAGG CAGCACACAG GATGGATAGC
    TTAGATTCCC ATGACAGTAT CTCCAGCTGT TACACTGAAG CAACCCTGCT GATCTCAAGG
    CAGTCCACCC TTGATGATTT AGATGGACAA GACCCTGTTC CTAAAACAAG CGAGCGTGCT
    CGGCCTAGGC TCCGTAAAGT GTTCAGCATG GACATGTCCT CCTCATCAGC TGACAGTGGC
    AGTGTAGCAT CAAGCGAGCC CACACAGTGC ACCATGCTGT ATTCACGCCA AGGAACTACG
    GAAACCATTG AGGAGGTAGA GGCTGAAGCT GAGGAGGAGG TGGTAGAGAG TCTGGAGCCT
    GAGCTCCATG ATGCCGAAGA GAAGGAATAT GCAGCTGACT ATGAAGCAGG GGTAGAGGAG
    GTCAGCCTCA CTCCAGATGA GGAGTTGCCT CAGTTCTCAA CAGATGACGG TGACGTGGAG
    GCCCCGCCCT CCTACAGCAA GGCTGTCAGC TTTGAGCACC TCTCGTTTGC ATCACAAGAT
    GACTCCGGGG GCAAGAACCA CATGGTGGTC AGCCCTGACG ACAGCCGCAC AGACAAGCTG
    GAGTCCAGTA TCTTACCGCC CCTGACTCAC GAGCTGACAG CCAGCGATCT GCTCATGAGC
    AAGATGTTCC GTGATGATGA GCTTGAAGAA TCTGAGAAAT TCTACGTGGA CCAGCCCCGG
    TTCTTGCTGC TATTCTATGC CATGTATAAC ACTCTGGTAG CCCGCTCGGA AATGGTGTGC
    TATTTCGTGA TCATCCTGAA CCACATGATC TCTGCCTCCA TCATTACTCT TCTGTTGCCC
    ATCCTGATCT TCCTCTGGGC CATGCTGTCC GTCCCCAGGC CCAGCCGGCG CTTCTGGATG
    ATGGCCATCG TCTACACTGA GGTGGCGATT GTAGTGAAAT ATTTCTTCCA GTTTGGCTTC
    TTTCCTTGGA ATAAGGACTT GGAAATTTAC AAGGACAGGC CCTACTTTCC ACCAAACATC
    ATAGGGGTGG AAAAGAAAGA GGGCTATGTT CTTTACGACC TCATTCAGCT GCTGGCTCTG
    TTCTTCCATC GATCCATTCT GAAGTGTCAT GGCCTATGGG ACGAGGATGA CATTGTTGAC
    AGTAACACAG ACAAGGAAGG ATCTGATGAT GAGCTGTCCC TCGGTCAGGG CAGAAGAGGC
    TCCTCAGAGT CACTGAAGTC CATCAACTTG GCTGCCTCTG TAGAGTCCGT CCATGTGACC
    TTCCCAGAGC AGCCAGCAGC CATCCGGAGA AAACGCTCCT GCAGCAGCTC GCAGATCTCC
    CCACGGTCCA GCTTCTCTTC AAATAGGTCC AAAAGAGGCA GCACAAGCAC CCGAAACAGC
    AGCCAAAAAG GAAGCAGTGT TTTGAGCATT AAACAAAAAA GCAAAAGAGA ACTTTACATG
    GAAAAGCTTC AAGAACATTT AATCAAAGCA AAGGCCTTTA CCATAAAGAA AACTCTACAA
    ATCTATGTAC CCATTCGGCA ATTCTTCTAT GACCTCATCC ACCCAGACTA CAGTGCTGTG
    ACTGACGTGT ATGTGCTGAT GTTCCTGGCC GACACCGTGG ACTTCATCAT CATCGTCTTT
    GGCTTCTGGG CTTTTGGGAA ACACTCGGCA GCTGCAGATA TCACCTCTTC CCTATCAGAA
    GACCAGGTCC CCGGGCCATT CCTGGTGATG GTTCTCATTC AGTTTGGAAC CATGGTGGTG
    GACCGTGCAC TCTACCTCAG GAAGACTGTG CTTGGCAAGG TCATTTTCCA GGTCATCCTC
    GTGTTTGGAA TTCACTTCTG GATGTTCTTC ATCCTACCCA GTGTGACTGA GAGGAAATTC
    AGCCAGAACC TGGTGGCACA GCTCTGGTAC TTTGTGAAAT GTGTGTACTT TGGGTTGTCT
    GCCTACCAAA TCCGCTGTGG CTACCCAACA CGTGTGCTCG GAAACTTCCT CACGAAGAGC
    TACAATTATG TCAACCTCTT CTTGTTTCAA GGGTTTCGTC TCGTCCCATT TCTGACAGAG
    CTGCGGGCTG TGATGGACTG GGTGTGGACA GATACAACCT TGAGCCTCTC CAGCTGGATT
    TGTGTGGAGG ACATTTACGC ACACATTTTC ATCTTAAAGT GCTGGCGAGA GTCGGAAAAG
    AGATACCCTC AACCCAGGGG CCAGAAGAAG AAGAAAGCTG TGAAGTATGG CATGGGCGGA
    ATGATCATTG TCCTGCTCAT CTGTATCGTC TGGTTTCCTC TGCTCTTCAT GTCTTTGATC
    AAGTCTGTCG CAGGGGTTAT TAACCAACCC CTGGATGTGT CCGTCACAAT AACCCTGGGA
    GGCTATCAGC CTATCTTCAC AATGAGTGCC CAGCAAAGCC AGCTGAAAGT TATGGATGGT
    ACAAAGTATA ACGAATTCAT AAAGTCTTTC GGCCCCAACA GTGGCGCTAT GCAATTTCTG
    GAGAATTATG AAAGGCAAGA CATAACAGTA GCGGAACTGG AAGGGAATTC GAATTCTTTG
    TGGACCATCA GCCCTCCCAG TAAGCAGAAA ATGATACAGG AGCTCACCGA CCCCAATAGT
    TGCTTCTCTG TTGTTTTTTC ATGGAGTATC CAGAGAAACA TGACTCTGGG TGCAAAGGCT
    GAAATAGCAA CGGACAAGCT TTCTTTTCCT CTCACGGATG CGACACGAAG TAGCATAGCC
    AAAATGATTG CTGGCAATGA CACAGAAAGT TCCAACACAC CAGTGACAAT AGAAAAGATC
    TACCCATACT ATGTGAAGGC ACCCAGTGAC TCAAACTCAA AGCCTATAAA ACAGCTTCTG
    TCAGAAAACA ATTTCATGAA TATCACCATC ATTTTGTTCA GAGACAATGT CACAAAGTCC
    AATAGTGAGT GGTGGGTTCT CAACCTGACT GGAAGTAGGA TATTCAATCA GGGATCCCAA
    GCCTTGGAGC TGGTGGTCTT CAATGACAAA GTCAGCCCCC CAAGTCTGGG CTTCTTGGCT
    GGCTATGGCA TCATGGGATT GTATGCATCC GTTGTCCTTG TAATTGGGAA GTTTGTTCGC
    GAATTCTTCA GTGGGATTTC TCACTCTATC ATGTTTGAAG AGCTTCCAAA TGTGGACAGA
    ATTTTGAAGT TATGCACAGA TATATTTCTC GTACGGGAGA CAGGGGAACT GGAGCTGGAG
    GAAGACCTCT ATGCCAAATT GATATTCCTG TACAGGTCGC CAGAAACCAT GATCAAGTGG
    ACTAGAGAAA AAACAAATTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa54716
    Clone ID Related Accession (Same CDS sequence) XM_017601114.1 , XM_017587875.1
    Accession Version XM_017587875.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8601bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product piezo-type mechanosensitive ion channel component 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000086.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
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    1141
    1201
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    1321
    1381
    1441
    1501
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    1981
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    7741
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    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTCCCCAT CTGCCTGGCA 
    GTAGCATGTG CATTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCG ACGATGCAAG GACACACAGG GCGGCTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCCTTT CTGCTCCTTC ACATCATTTT CCACATCACA
    CTGGCCAGCC TGGAAGCTCA GCACCGCATC GCACCCGCTT ACAACTGCTC AACTTGGGAA
    AAGACCTTCC GGCAGATCGG CTTCGAAAGC TTGAAGGGAG CTGATGCTGG CAATGGCATC
    AGGGTGTTTG TGCCTGATAT CGGGATGTTC ATTGCGAGCC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACGGAA GAAGTAGCCC AATTTAATTC CGAGTGCGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GACTCAGAAG AAGCGCTGAT TTATGAGGAG
    GACTTAGATG GGGAAGAAGG CATGGAAGGC GAGCTGGAAG AAAGCACAAA GTTAAAAATG
    TTTCGCAGGC TCGCCTCCGT CGCTTCCAAG CTTAAGGAGT TCATCGGCAA TATGATAACC
    ACGGCGGGCA AGGTGGTGGT GACCATCCTG TTGGGCTCCT CTGGCATGAT GCTGCCATCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT CTGGGTCTGT GCACCTGGTG GTCCTGGTGC
    CGAACTTTTG ACCCATTGCT GTTTGGCTGC CTCTGTGTCC TGTTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTTCAGTTCT TCCAAGAAGC AGTCCCACCC
    AATGACTACT ATGCAAGGTT GTTTGGAATC AAATCCGTTA TTCAAACAGA CTGCTCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTACAC GCTGGCCACG CTGATCCGCA TTTGGTTGCA AGAGCCTCTT
    GTGCAGGAGG AGATGTCGAA GGAAGAGGAG GGTGCACTGG ATGGCTGCTC CAATCAGAAC
    ACAGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    CTTTTATCCA TGACCCAAGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACTGTGAAC
    GGCAACCCTG TGGACTACCA CACCATCCAC CCAAGTCTGC CCATAGAGAA TGGCCCCGCC
    AAGACGGACT TGTATACTAC TCCTCAGTAC CGGTGGGAGC CCTCAGAGGA GTCCTCAGAA
    AAGAAAGAGG AGGAAGAGGA CAAGAGAGAA GACTCAGAAG GGGAAGGGAG CCAAGAGGAA
    AAACGGAGTG TGAGAATTCA TGCCATGGTC GCCGTGTTCC AGTTCATCAT GAAGCAGAGT
    TACATCTGTG CCCTCATCGC CATGATGGCA TGGAGCATCA CCTACCACAG CTGGCTGACA
    TTTGTGCTGT TGATCTGGTC GTGCACTCTC TGGATGATTC GCAACAGGAG GAAGTACGCC
    ATGATCAGCT CTCCGTTCAT GGTTGTCTAT GCCAATCTGC TGCTGGTACT GCAGTACATA
    TGGAGCTTTG AACTTCCAGA AATCAAGAAA GTCCCTGGAT TTCTAGAAAA GAAAGAGCCT
    GGAGAGCTTG CCTCAAAGAT CCTCTTCACC ATCACATTTT GGCTGCTCCT GAGGCAGCAT
    CTCACAGAGC AGAAGGCCCT TCAGGAAAAG GAAGCACTTC TGTCTGAAGT CAAAATTGGA
    AGTCAAGAAC TTGACGAAAA GGATGAGGAA CTCCAAGATA TACAAGTGGA AGGAGAGCCC
    ATAGAGAAGG AAGAAGAGGA GGAGGAAATA AAGGAAGACA GACATGAGGT AAAGAAGGAA
    GAGGAAGAGG AAGAGGAAGA TGACGATGAC CAGGACATCA TGAAGGTTCT GGGCAATCTG
    GTGGTGGCCC TCTTCATCAA GTATTGGATC TACGTCTGCG GGGGCATGTT CTTTTTTGTC
    AGCTTTGAGG GGAAAATCGT CATGTACAAA ATCATCTACA TGGTGCTGTT CCTGTTCTGT
    GTGGCCTTGT ACCAGGTGCA CTACGAGTGG TGGAGGAAGA TCCTGAAATA CTTCTGGATG
    TCGGTGGTCA TTTACACCAT GCTGGTGCTC ATCTTCATCT ACACTTACCA GTTTGAGAAC
    TTCCCAGGCC TGTGGCAAAA CATGACCGGG TTAAAGAAAG AGAAGCTTGA GGATCTGGGC
    CTGAAGCAGT TCACTGTGGC CGAACTGTTC ACACGAATAT TCATCCCCAC CTCCTTTCTG
    CTGGTCTGCA TCCTGCACCT CCACTATTTC CATGACCGGT TCCTGGAACT CACAGACCTC
    AAGTCCATCC CCAGCAAAGA AGACAACACC ATCTACAGCC ATGCCAAGGT CAATGGTCGC
    GTGTACCTAA TAATAAATAG CATCAAGAAA AAATTACCAA TCCACCAGAA TGAGTTAGCC
    CACCCTGAAG GAAGCCTCCC AGACCTAGCC ATTATGAACA TGACGGCCAG CCTCGATAAG
    CCTGAGAGGC AGAAGTTGGC AGAGCCTGGG GAGGAGAGGA CAGAAGAATG TGCCAAGAAG
    GCAGAGAAGG GTGAGGCTGG GAAAGACAGT GATGAGTCTG AGGAGGAGGA AGGTGAGGAA
    GAGGAATCCG AGGAGGAAGA ATCCTCAGAT CTCAGGAACA AGTGGCACCT CGTGATTGAC
    CGGCTCACTG TGCTCTTTCT AAAGTTCCTG GAGTATTTCC ACAAGTTGCA GGTGTTTATG
    TGGTGGATCT TGGAGCTGCA CATCATCAAA ATCGTCTCAT CGTACATTAT CTGGGTGACT
    GTCAAAGAGG TGTCTCTGTT CAACTATGTA TTTTTGATTT CTTGGGCATT TGCTCTGCCA
    TATGCCAAAC TCCGCCGTGT GGCTTCCAGT GTCTGCACTG TCTGGACGTG TGTGATCATC
    GTCTGCAAAA TGTTGTACCA GCTGCAAACC ATTAAGCCTG AGAACTTTTC TGTCAACTGC
    TCCTTGCCCA ATGAAAATCA AACCAACATA CCCCTTAATG AGTTGAACAA GTCTCTTCTC
    TACAGTGCCC CTGTTGATCC CACTGAGTGG GTTGGCCTGA GGAAGTCCTC CCCTTTACTC
    GTCTACCTGA GGAACAACCT CCTGATGTTG GCGATTTTGG CCTTCGAAGT CACTGTTTAC
    CGACATCAGG AGTACTATCG AGGCCGAAAC AACTTGACTG CTCCGGTGTC TAAAACTATC
    TTTCATGACA TCACAAGGTT ACATCTAGAT GATGGGCTTA TCAATTGTGC CAAATATTTT
    GTAAACTACT TCTTCTACAA GTTTGGCCTA GAGACCTGTT TCCTAATGTC AGTGAACGTA
    ATTGGACAGC GAATGGACTT CTACGCCATG ATTCATGCCT GTTGGCTGAT CGGTGTCTTG
    TACCGTCGCA GAAGAAAAGC CATTGCTGAG GTCTGGCCCA AGTACTGCTG CTTCCTGGCT
    TGCATCATCA CCTTCCAGTA CTTTGTCTGC ATAGGCATCC CTCCTGCCCC CTGCCGAGAT
    TACCCATGGA GGTTCAAGGG TGCCAACTTC AATGACAACA TCATCAAGTG GCTGTACTTC
    CCAGATTTCA TCGTGCGGCC CAACCCTGTG TTTCTTGTCT ATGACTTCAT GCTGCTCCTG
    TGTGCTTCCT TACAACGACA GATTTTTGAG GACGAAAACA AGGCAGCTGT GCGCATCATG
    GCCGGGGACA ATGTCGAGAT TTGCATGAAC CTTGACGCGG CCTCATTCAG CCAGCATAAC
    CCTGTACCAG ATTTCATTCA CTGCAGATCT TACTTGGACA TGTCCAAAGT GATCATCTTC
    AGCTACCTCT TCTGGTTCGT CCTCACCATC ATCTTCATTA CCGGAACGAC CAGGATCAGC
    ATCTTTTGCA TGGGTTACCT GGTGGCCTGT TTCTATTTCC TGCTCTTTGG AGGTGATCTG
    CTCCTGAAAC CCATCAAGAG CATCCTACGC TACTGGGACT GGCTGATTGC ATACAACGTG
    TTTGTGATCA CCATGAAAAA CATACTGTCA ATAGGAGCCT GTGGATACAT CGGTGCACTG
    GTGAGAAACA GCTGCTGGCT GATCCAAGCG TTCAGCCTGG CCTGCACGGT GAAAGGCTAT
    AAAATGCCTG AAGATGACTC CAGATGCAAG CTGCCCAGTG GGGAGGCAGG GATCATCTGG
    GACAGCATCT GCTTTGCGTT TCTCCTGCTA CAGAGGAGAG TCTTCATGAG TTACTATTTC
    CTGCATGTCG TGGCTGACAT TAAAGCATCC CAGATCCTGG CATCCAGAGG GGCCGAACTT
    TTCCAGGCTA CAATTGTGAA AGCTGTAAAG GCAAGAATTG AAGAAGAGAA AAAATCCATG
    GACCAGCTGA AGAGACAGAT GGATCGCATC AAAGCCCGGC AGCAAAAGTA CAAAAAGGGT
    AAGGAGAGGA TGCTGAGCTT GACCCAGGAG TCAGGGGAAG GCCAGGACAT CCAGAAAGTC
    TCAGAAGAAG ACGATGAAAG AGAAGCAGAC AAACAGAAAG CCAAGGGCAA AAAAAAGCAG
    TGGTGGCGGC CTTGGGTTGA TCATGCTTCC ATGGTCAGGA GTGGAGATTA TTATTTGTTT
    GAAACGGATA GTGAAGAGGA GGAAGAAGAG GAACTCAAAA AGGAGGATGA AGAACCACCA
    CGGAAGTCTG CATTCCAGAG AGCTATTGGG AAGTTTGCCT CAGCCATTTT AGCCTTGCCA
    AAGTCTGTCA TTAAACTTCC CAAAACTATT CTTCAGTATT TAATCAGAGC TGCAAAGTTC
    GTCTACCAAG CCTGGATTAC CGATCCTAAA ACAGCACTCC GACAGAGGAG GAAAGAGAAG
    AAGAAAATGG CCAGAGAAGA GCAGAAGGGA AGACGCAAAG GATCTGGGGA GGGTCCAGTG
    GAATGGGAAG ACAGAGAAGA TGAGCCAATC AAAAAGAAAT CTGATGGACC AGATAATATC
    ATCAAGAGGA TATTTAATAT CCTGAAGTTC ACCTGGGTCC TGTTCCTGGC CACAGTGGAC
    AGTTTCACTA CTTGGCTCAA CTCCATCTCA AGAGAGCATA TCGACATATC CACAGTTCTG
    CGGATTGAAA GATGCATGCT GACCCGAGAG ATAAAGAAGG GCAACGTTCC GACACGGGAA
    AGCATCCACA TGTACTACCA GAACCACATG ATGAACCTTT CCAGAGAATC CGGACTGGAC
    ACAATCGACG AGCACTCTGG CGCTGGTTCC AGGGCCCAGG CAGCACACAG GATGGATAGC
    TTAGATTCCC ATGACAGTAT CTCCAGCTGT TACACTGAAG CAACCCTGCT GATCTCAAGG
    CAGTCCACCC TTGATGATTT AGATGGACAA GACCCTGTTC CTAAAACAAG CGAGCGTGCT
    CGGCCTAGGC TCCGTAAAGT GTTCAGCATG GACATGTCCT CCTCATCAGC TGACAGTGGC
    AGTGTAGCAT CAAGCGAGCC CACACAGTGC ACCATGCTGT ATTCACGCCA AGGAACTACG
    GAAACCATTG AGGAGGTAGA GGCTGAAGCT GAGGAGGAGG TGGTAGAGAG TCTGGAGCCT
    GAGCTCCATG ATGCCGAAGA GAAGGAATAT GCAGCTGACT ATGAAGCAGG GGTAGAGGAG
    GTCAGCCTCA CTCCAGATGA GGAGTTGCCT CAGTTCTCAA CAGATGACGG TGACGTGGAG
    GCCCCGCCCT CCTACAGCAA GGCTGTCAGC TTTGAGCACC TCTCGTTTGC ATCACAAGAT
    GACTCCGGGG GCAAGAACCA CATGGTGGTC AGCCCTGACG ACAGCCGCAC AGACAAGCTG
    GAGTCCAGTA TCTTACCGCC CCTGACTCAC GAGCTGACAG CCAGCGATCT GCTCATGAGC
    AAGATGTTCC GTGATGATGA GCTTGAAGAA TCTGAGAAAT TCTACGTGGA CCAGCCCCGG
    TTCTTGCTGC TATTCTATGC CATGTATAAC ACTCTGGTAG CCCGCTCGGA AATGGTGTGC
    TATTTCGTGA TCATCCTGAA CCACATGATC TCTGCCTCCA TCATTACTCT TCTGTTGCCC
    ATCCTGATCT TCCTCTGGGC CATGCTGTCC GTCCCCAGGC CCAGCCGGCG CTTCTGGATG
    ATGGCCATCG TCTACACTGA GGTGGCGATT GTAGTGAAAT ATTTCTTCCA GTTTGGCTTC
    TTTCCTTGGA ATAAGGACTT GGAAATTTAC AAGGACAGGC CCTACTTTCC ACCAAACATC
    ATAGGGGTGG AAAAGAAAGA GGGCTATGTT CTTTACGACC TCATTCAGCT GCTGGCTCTG
    TTCTTCCATC GATCCATTCT GAAGTGTCAT GGCCTATGGG ACGAGGATGA CATTGTTGAC
    AGTAACACAG ACAAGGAAGG ATCTGATGAT GAGCTGTCCC TCGGTCAGGG CAGAAGAGGC
    TCCTCAGAGT CACTGAAGTC CATCAACTTG GCTGCCTCTG TAGAGTCCGT CCATGTGACC
    TTCCCAGAGC AGCCAGCAGC CATCCGGAGA AAACGCTCCT GCAGCAGCTC GCAGATCTCC
    CCACGGTCCA GCTTCTCTTC AAATAGGTCC AAAAGAGGCA GCACAAGCAC CCGAAACAGC
    AGCCAAAAAG GAAGCAGTGT TTTGAGCATT AAACAAAAAA GCAAAAGAGA ACTTTACATG
    GAAAAGCTTC AAGAACATTT AATCAAAGCA AAGGCCTTTA CCATAAAGAA AACTCTACAA
    ATCTATGTAC CCATTCGGCA ATTCTTCTAT GACCTCATCC ACCCAGACTA CAGTGCTGTG
    ACTGACGTGT ATGTGCTGAT GTTCCTGGCC GACACCGTGG ACTTCATCAT CATCGTCTTT
    GGCTTCTGGG CTTTTGGGAA ACACTCGGCA GCTGCAGATA TCACCTCTTC CCTATCAGAA
    GACCAGGTCC CCGGGCCATT CCTGGTGATG GTTCTCATTC AGTTTGGAAC CATGGTGGTG
    GACCGTGCAC TCTACCTCAG GAAGACTGTG CTTGGCAAGG TCATTTTCCA GGTCATCCTC
    GTGTTTGGAA TTCACTTCTG GATGTTCTTC ATCCTACCCA GTGTGACTGA GAGGAAATTC
    AGCCAGAACC TGGTGGCACA GCTCTGGTAC TTTGTGAAAT GTGTGTACTT TGGGTTGTCT
    GCCTACCAAA TCCGCTGTGG CTACCCAACA CGTGTGCTCG GAAACTTCCT CACGAAGAGC
    TACAATTATG TCAACCTCTT CTTGTTTCAA GGGTTTCGTC TCGTCCCATT TCTGACAGAG
    CTGCGGGCTG TGATGGACTG GGTGTGGACA GATACAACCT TGAGCCTCTC CAGCTGGATT
    TGTGTGGAGG ACATTTACGC ACACATTTTC ATCTTAAAGT GCTGGCGAGA GTCGGAAAAG
    AGATACCCTC AACCCAGGGG CCAGAAGAAG AAGAAAGCTG TGAAGTATGG CATGGGCGGA
    ATGATCATTG TCCTGCTCAT CTGTATCGTC TGGTTTCCTC TGCTCTTCAT GTCTTTGATC
    AAGTCTGTCG CAGGGGTTAT TAACCAACCC CTGGATGTGT CCGTCACAAT AACCCTGGGA
    GGCTATCAGC CTATCTTCAC AATGAGTGCC CAGCAAAGCC AGCTGAAAGT TATGGATGGT
    ACAAAGTATA ACGAATTCAT AAAGTCTTTC GGCCCCAACA GTGGCGCTAT GCAATTTCTG
    GAGAATTATG AAAGGCAAGA CATAACAGTA GCGGAACTGG AAGGGAATTC GAATTCTTTG
    TGGACCATCA GCCCTCCCAG TAAGCAGAAA ATGATACAGG AGCTCACCGA CCCCAATAGT
    TGCTTCTCTG TTGTTTTTTC ATGGAGTATC CAGAGAAACA TGACTCTGGG TGCAAAGGCT
    GAAATAGCAA CGGACAAGCT TTCTTTTCCT CTCACGGATG CGACACGAAG TAGCATAGCC
    AAAATGATTG CTGGCAATGA CACAGAAAGT TCCAACACAC CAGTGACAAT AGAAAAGATC
    TACCCATACT ATGTGAAGGC ACCCAGTGAC TCAAACTCAA AGCCTATAAA ACAGCTTCTG
    TCAGAAAACA ATTTCATGAA TATCACCATC ATTTTGTTCA GAGACAATGT CACAAAGTCC
    AATAGTGAGT GGTGGGTTCT CAACCTGACT GGAAGTAGGA TATTCAATCA GGGATCCCAA
    GCCTTGGAGC TGGTGGTCTT CAATGACAAA GTCAGCCCCC CAAGTCTGGG CTTCTTGGCT
    GGCTATGGCA TCATGGGATT GTATGCATCC GTTGTCCTTG TAATTGGGAA GTTTGTTCGC
    GAATTCTTCA GTGGGATTTC TCACTCTATC ATGTTTGAAG AGCTTCCAAA TGTGGACAGA
    ATTTTGAAGT TATGCACAGA TATATTTCTC GTACGGGAGA CAGGGGAACT GGAGCTGGAG
    GAAGACCTCT ATGCCAAATT GATATTCCTG TACAGGTCGC CAGAAACCAT GATCAAGTGG
    ACTAGAGAAA AAACAAATTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017443364.1
    CDS940..9540
    Translation

    Target ORF information:

    RefSeq Version XM_017587875.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017587875.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTCCCCAT CTGCCTGGCA 
    GTAGCATGTG CATTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCG ACGATGCAAG GACACACAGG GCGGCTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCCTTT CTGCTCCTTC ACATCATTTT CCACATCACA
    CTGGCCAGCC TGGAAGCTCA GCACCGCATC GCACCCGCTT ACAACTGCTC AACTTGGGAA
    AAGACCTTCC GGCAGATCGG CTTCGAAAGC TTGAAGGGAG CTGATGCTGG CAATGGCATC
    AGGGTGTTTG TGCCTGATAT CGGGATGTTC ATTGCGAGCC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACGGAA GAAGTAGCCC AATTTAATTC CGAGTGCGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GACTCAGAAG AAGCGCTGAT TTATGAGGAG
    GACTTAGATG GGGAAGAAGG CATGGAAGGC GAGCTGGAAG AAAGCACAAA GTTAAAAATG
    TTTCGCAGGC TCGCCTCCGT CGCTTCCAAG CTTAAGGAGT TCATCGGCAA TATGATAACC
    ACGGCGGGCA AGGTGGTGGT GACCATCCTG TTGGGCTCCT CTGGCATGAT GCTGCCATCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT CTGGGTCTGT GCACCTGGTG GTCCTGGTGC
    CGAACTTTTG ACCCATTGCT GTTTGGCTGC CTCTGTGTCC TGTTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTTCAGTTCT TCCAAGAAGC AGTCCCACCC
    AATGACTACT ATGCAAGGTT GTTTGGAATC AAATCCGTTA TTCAAACAGA CTGCTCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTACAC GCTGGCCACG CTGATCCGCA TTTGGTTGCA AGAGCCTCTT
    GTGCAGGAGG AGATGTCGAA GGAAGAGGAG GGTGCACTGG ATGGCTGCTC CAATCAGAAC
    ACAGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    CTTTTATCCA TGACCCAAGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACTGTGAAC
    GGCAACCCTG TGGACTACCA CACCATCCAC CCAAGTCTGC CCATAGAGAA TGGCCCCGCC
    AAGACGGACT TGTATACTAC TCCTCAGTAC CGGTGGGAGC CCTCAGAGGA GTCCTCAGAA
    AAGAAAGAGG AGGAAGAGGA CAAGAGAGAA GACTCAGAAG GGGAAGGGAG CCAAGAGGAA
    AAACGGAGTG TGAGAATTCA TGCCATGGTC GCCGTGTTCC AGTTCATCAT GAAGCAGAGT
    TACATCTGTG CCCTCATCGC CATGATGGCA TGGAGCATCA CCTACCACAG CTGGCTGACA
    TTTGTGCTGT TGATCTGGTC GTGCACTCTC TGGATGATTC GCAACAGGAG GAAGTACGCC
    ATGATCAGCT CTCCGTTCAT GGTTGTCTAT GCCAATCTGC TGCTGGTACT GCAGTACATA
    TGGAGCTTTG AACTTCCAGA AATCAAGAAA GTCCCTGGAT TTCTAGAAAA GAAAGAGCCT
    GGAGAGCTTG CCTCAAAGAT CCTCTTCACC ATCACATTTT GGCTGCTCCT GAGGCAGCAT
    CTCACAGAGC AGAAGGCCCT TCAGGAAAAG GAAGCACTTC TGTCTGAAGT CAAAATTGGA
    AGTCAAGAAC TTGACGAAAA GGATGAGGAA CTCCAAGATA TACAAGTGGA AGGAGAGCCC
    ATAGAGAAGG AAGAAGAGGA GGAGGAAATA AAGGAAGACA GACATGAGGT AAAGAAGGAA
    GAGGAAGAGG AAGAGGAAGA TGACGATGAC CAGGACATCA TGAAGGTTCT GGGCAATCTG
    GTGGTGGCCC TCTTCATCAA GTATTGGATC TACGTCTGCG GGGGCATGTT CTTTTTTGTC
    AGCTTTGAGG GGAAAATCGT CATGTACAAA ATCATCTACA TGGTGCTGTT CCTGTTCTGT
    GTGGCCTTGT ACCAGGTGCA CTACGAGTGG TGGAGGAAGA TCCTGAAATA CTTCTGGATG
    TCGGTGGTCA TTTACACCAT GCTGGTGCTC ATCTTCATCT ACACTTACCA GTTTGAGAAC
    TTCCCAGGCC TGTGGCAAAA CATGACCGGG TTAAAGAAAG AGAAGCTTGA GGATCTGGGC
    CTGAAGCAGT TCACTGTGGC CGAACTGTTC ACACGAATAT TCATCCCCAC CTCCTTTCTG
    CTGGTCTGCA TCCTGCACCT CCACTATTTC CATGACCGGT TCCTGGAACT CACAGACCTC
    AAGTCCATCC CCAGCAAAGA AGACAACACC ATCTACAGCC ATGCCAAGGT CAATGGTCGC
    GTGTACCTAA TAATAAATAG CATCAAGAAA AAATTACCAA TCCACCAGAA TGAGTTAGCC
    CACCCTGAAG GAAGCCTCCC AGACCTAGCC ATTATGAACA TGACGGCCAG CCTCGATAAG
    CCTGAGAGGC AGAAGTTGGC AGAGCCTGGG GAGGAGAGGA CAGAAGAATG TGCCAAGAAG
    GCAGAGAAGG GTGAGGCTGG GAAAGACAGT GATGAGTCTG AGGAGGAGGA AGGTGAGGAA
    GAGGAATCCG AGGAGGAAGA ATCCTCAGAT CTCAGGAACA AGTGGCACCT CGTGATTGAC
    CGGCTCACTG TGCTCTTTCT AAAGTTCCTG GAGTATTTCC ACAAGTTGCA GGTGTTTATG
    TGGTGGATCT TGGAGCTGCA CATCATCAAA ATCGTCTCAT CGTACATTAT CTGGGTGACT
    GTCAAAGAGG TGTCTCTGTT CAACTATGTA TTTTTGATTT CTTGGGCATT TGCTCTGCCA
    TATGCCAAAC TCCGCCGTGT GGCTTCCAGT GTCTGCACTG TCTGGACGTG TGTGATCATC
    GTCTGCAAAA TGTTGTACCA GCTGCAAACC ATTAAGCCTG AGAACTTTTC TGTCAACTGC
    TCCTTGCCCA ATGAAAATCA AACCAACATA CCCCTTAATG AGTTGAACAA GTCTCTTCTC
    TACAGTGCCC CTGTTGATCC CACTGAGTGG GTTGGCCTGA GGAAGTCCTC CCCTTTACTC
    GTCTACCTGA GGAACAACCT CCTGATGTTG GCGATTTTGG CCTTCGAAGT CACTGTTTAC
    CGACATCAGG AGTACTATCG AGGCCGAAAC AACTTGACTG CTCCGGTGTC TAAAACTATC
    TTTCATGACA TCACAAGGTT ACATCTAGAT GATGGGCTTA TCAATTGTGC CAAATATTTT
    GTAAACTACT TCTTCTACAA GTTTGGCCTA GAGACCTGTT TCCTAATGTC AGTGAACGTA
    ATTGGACAGC GAATGGACTT CTACGCCATG ATTCATGCCT GTTGGCTGAT CGGTGTCTTG
    TACCGTCGCA GAAGAAAAGC CATTGCTGAG GTCTGGCCCA AGTACTGCTG CTTCCTGGCT
    TGCATCATCA CCTTCCAGTA CTTTGTCTGC ATAGGCATCC CTCCTGCCCC CTGCCGAGAT
    TACCCATGGA GGTTCAAGGG TGCCAACTTC AATGACAACA TCATCAAGTG GCTGTACTTC
    CCAGATTTCA TCGTGCGGCC CAACCCTGTG TTTCTTGTCT ATGACTTCAT GCTGCTCCTG
    TGTGCTTCCT TACAACGACA GATTTTTGAG GACGAAAACA AGGCAGCTGT GCGCATCATG
    GCCGGGGACA ATGTCGAGAT TTGCATGAAC CTTGACGCGG CCTCATTCAG CCAGCATAAC
    CCTGTACCAG ATTTCATTCA CTGCAGATCT TACTTGGACA TGTCCAAAGT GATCATCTTC
    AGCTACCTCT TCTGGTTCGT CCTCACCATC ATCTTCATTA CCGGAACGAC CAGGATCAGC
    ATCTTTTGCA TGGGTTACCT GGTGGCCTGT TTCTATTTCC TGCTCTTTGG AGGTGATCTG
    CTCCTGAAAC CCATCAAGAG CATCCTACGC TACTGGGACT GGCTGATTGC ATACAACGTG
    TTTGTGATCA CCATGAAAAA CATACTGTCA ATAGGAGCCT GTGGATACAT CGGTGCACTG
    GTGAGAAACA GCTGCTGGCT GATCCAAGCG TTCAGCCTGG CCTGCACGGT GAAAGGCTAT
    AAAATGCCTG AAGATGACTC CAGATGCAAG CTGCCCAGTG GGGAGGCAGG GATCATCTGG
    GACAGCATCT GCTTTGCGTT TCTCCTGCTA CAGAGGAGAG TCTTCATGAG TTACTATTTC
    CTGCATGTCG TGGCTGACAT TAAAGCATCC CAGATCCTGG CATCCAGAGG GGCCGAACTT
    TTCCAGGCTA CAATTGTGAA AGCTGTAAAG GCAAGAATTG AAGAAGAGAA AAAATCCATG
    GACCAGCTGA AGAGACAGAT GGATCGCATC AAAGCCCGGC AGCAAAAGTA CAAAAAGGGT
    AAGGAGAGGA TGCTGAGCTT GACCCAGGAG TCAGGGGAAG GCCAGGACAT CCAGAAAGTC
    TCAGAAGAAG ACGATGAAAG AGAAGCAGAC AAACAGAAAG CCAAGGGCAA AAAAAAGCAG
    TGGTGGCGGC CTTGGGTTGA TCATGCTTCC ATGGTCAGGA GTGGAGATTA TTATTTGTTT
    GAAACGGATA GTGAAGAGGA GGAAGAAGAG GAACTCAAAA AGGAGGATGA AGAACCACCA
    CGGAAGTCTG CATTCCAGAG AGCTATTGGG AAGTTTGCCT CAGCCATTTT AGCCTTGCCA
    AAGTCTGTCA TTAAACTTCC CAAAACTATT CTTCAGTATT TAATCAGAGC TGCAAAGTTC
    GTCTACCAAG CCTGGATTAC CGATCCTAAA ACAGCACTCC GACAGAGGAG GAAAGAGAAG
    AAGAAAATGG CCAGAGAAGA GCAGAAGGGA AGACGCAAAG GATCTGGGGA GGGTCCAGTG
    GAATGGGAAG ACAGAGAAGA TGAGCCAATC AAAAAGAAAT CTGATGGACC AGATAATATC
    ATCAAGAGGA TATTTAATAT CCTGAAGTTC ACCTGGGTCC TGTTCCTGGC CACAGTGGAC
    AGTTTCACTA CTTGGCTCAA CTCCATCTCA AGAGAGCATA TCGACATATC CACAGTTCTG
    CGGATTGAAA GATGCATGCT GACCCGAGAG ATAAAGAAGG GCAACGTTCC GACACGGGAA
    AGCATCCACA TGTACTACCA GAACCACATG ATGAACCTTT CCAGAGAATC CGGACTGGAC
    ACAATCGACG AGCACTCTGG CGCTGGTTCC AGGGCCCAGG CAGCACACAG GATGGATAGC
    TTAGATTCCC ATGACAGTAT CTCCAGCTGT TACACTGAAG CAACCCTGCT GATCTCAAGG
    CAGTCCACCC TTGATGATTT AGATGGACAA GACCCTGTTC CTAAAACAAG CGAGCGTGCT
    CGGCCTAGGC TCCGTAAAGT GTTCAGCATG GACATGTCCT CCTCATCAGC TGACAGTGGC
    AGTGTAGCAT CAAGCGAGCC CACACAGTGC ACCATGCTGT ATTCACGCCA AGGAACTACG
    GAAACCATTG AGGAGGTAGA GGCTGAAGCT GAGGAGGAGG TGGTAGAGAG TCTGGAGCCT
    GAGCTCCATG ATGCCGAAGA GAAGGAATAT GCAGCTGACT ATGAAGCAGG GGTAGAGGAG
    GTCAGCCTCA CTCCAGATGA GGAGTTGCCT CAGTTCTCAA CAGATGACGG TGACGTGGAG
    GCCCCGCCCT CCTACAGCAA GGCTGTCAGC TTTGAGCACC TCTCGTTTGC ATCACAAGAT
    GACTCCGGGG GCAAGAACCA CATGGTGGTC AGCCCTGACG ACAGCCGCAC AGACAAGCTG
    GAGTCCAGTA TCTTACCGCC CCTGACTCAC GAGCTGACAG CCAGCGATCT GCTCATGAGC
    AAGATGTTCC GTGATGATGA GCTTGAAGAA TCTGAGAAAT TCTACGTGGA CCAGCCCCGG
    TTCTTGCTGC TATTCTATGC CATGTATAAC ACTCTGGTAG CCCGCTCGGA AATGGTGTGC
    TATTTCGTGA TCATCCTGAA CCACATGATC TCTGCCTCCA TCATTACTCT TCTGTTGCCC
    ATCCTGATCT TCCTCTGGGC CATGCTGTCC GTCCCCAGGC CCAGCCGGCG CTTCTGGATG
    ATGGCCATCG TCTACACTGA GGTGGCGATT GTAGTGAAAT ATTTCTTCCA GTTTGGCTTC
    TTTCCTTGGA ATAAGGACTT GGAAATTTAC AAGGACAGGC CCTACTTTCC ACCAAACATC
    ATAGGGGTGG AAAAGAAAGA GGGCTATGTT CTTTACGACC TCATTCAGCT GCTGGCTCTG
    TTCTTCCATC GATCCATTCT GAAGTGTCAT GGCCTATGGG ACGAGGATGA CATTGTTGAC
    AGTAACACAG ACAAGGAAGG ATCTGATGAT GAGCTGTCCC TCGGTCAGGG CAGAAGAGGC
    TCCTCAGAGT CACTGAAGTC CATCAACTTG GCTGCCTCTG TAGAGTCCGT CCATGTGACC
    TTCCCAGAGC AGCCAGCAGC CATCCGGAGA AAACGCTCCT GCAGCAGCTC GCAGATCTCC
    CCACGGTCCA GCTTCTCTTC AAATAGGTCC AAAAGAGGCA GCACAAGCAC CCGAAACAGC
    AGCCAAAAAG GAAGCAGTGT TTTGAGCATT AAACAAAAAA GCAAAAGAGA ACTTTACATG
    GAAAAGCTTC AAGAACATTT AATCAAAGCA AAGGCCTTTA CCATAAAGAA AACTCTACAA
    ATCTATGTAC CCATTCGGCA ATTCTTCTAT GACCTCATCC ACCCAGACTA CAGTGCTGTG
    ACTGACGTGT ATGTGCTGAT GTTCCTGGCC GACACCGTGG ACTTCATCAT CATCGTCTTT
    GGCTTCTGGG CTTTTGGGAA ACACTCGGCA GCTGCAGATA TCACCTCTTC CCTATCAGAA
    GACCAGGTCC CCGGGCCATT CCTGGTGATG GTTCTCATTC AGTTTGGAAC CATGGTGGTG
    GACCGTGCAC TCTACCTCAG GAAGACTGTG CTTGGCAAGG TCATTTTCCA GGTCATCCTC
    GTGTTTGGAA TTCACTTCTG GATGTTCTTC ATCCTACCCA GTGTGACTGA GAGGAAATTC
    AGCCAGAACC TGGTGGCACA GCTCTGGTAC TTTGTGAAAT GTGTGTACTT TGGGTTGTCT
    GCCTACCAAA TCCGCTGTGG CTACCCAACA CGTGTGCTCG GAAACTTCCT CACGAAGAGC
    TACAATTATG TCAACCTCTT CTTGTTTCAA GGGTTTCGTC TCGTCCCATT TCTGACAGAG
    CTGCGGGCTG TGATGGACTG GGTGTGGACA GATACAACCT TGAGCCTCTC CAGCTGGATT
    TGTGTGGAGG ACATTTACGC ACACATTTTC ATCTTAAAGT GCTGGCGAGA GTCGGAAAAG
    AGATACCCTC AACCCAGGGG CCAGAAGAAG AAGAAAGCTG TGAAGTATGG CATGGGCGGA
    ATGATCATTG TCCTGCTCAT CTGTATCGTC TGGTTTCCTC TGCTCTTCAT GTCTTTGATC
    AAGTCTGTCG CAGGGGTTAT TAACCAACCC CTGGATGTGT CCGTCACAAT AACCCTGGGA
    GGCTATCAGC CTATCTTCAC AATGAGTGCC CAGCAAAGCC AGCTGAAAGT TATGGATGGT
    ACAAAGTATA ACGAATTCAT AAAGTCTTTC GGCCCCAACA GTGGCGCTAT GCAATTTCTG
    GAGAATTATG AAAGGCAAGA CATAACAGTA GCGGAACTGG AAGGGAATTC GAATTCTTTG
    TGGACCATCA GCCCTCCCAG TAAGCAGAAA ATGATACAGG AGCTCACCGA CCCCAATAGT
    TGCTTCTCTG TTGTTTTTTC ATGGAGTATC CAGAGAAACA TGACTCTGGG TGCAAAGGCT
    GAAATAGCAA CGGACAAGCT TTCTTTTCCT CTCACGGATG CGACACGAAG TAGCATAGCC
    AAAATGATTG CTGGCAATGA CACAGAAAGT TCCAACACAC CAGTGACAAT AGAAAAGATC
    TACCCATACT ATGTGAAGGC ACCCAGTGAC TCAAACTCAA AGCCTATAAA ACAGCTTCTG
    TCAGAAAACA ATTTCATGAA TATCACCATC ATTTTGTTCA GAGACAATGT CACAAAGTCC
    AATAGTGAGT GGTGGGTTCT CAACCTGACT GGAAGTAGGA TATTCAATCA GGGATCCCAA
    GCCTTGGAGC TGGTGGTCTT CAATGACAAA GTCAGCCCCC CAAGTCTGGG CTTCTTGGCT
    GGCTATGGCA TCATGGGATT GTATGCATCC GTTGTCCTTG TAATTGGGAA GTTTGTTCGC
    GAATTCTTCA GTGGGATTTC TCACTCTATC ATGTTTGAAG AGCTTCCAAA TGTGGACAGA
    ATTTTGAAGT TATGCACAGA TATATTTCTC GTACGGGAGA CAGGGGAACT GGAGCTGGAG
    GAAGACCTCT ATGCCAAATT GATATTCCTG TACAGGTCGC CAGAAACCAT GATCAAGTGG
    ACTAGAGAAA AAACAAATTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa54717
    Clone ID Related Accession (Same CDS sequence) XM_017601115.1 , XM_017587876.1
    Accession Version XM_017601115.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8568bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product piezo-type mechanosensitive ion channel component 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005117.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTCCCCAT CTGCCTGGCA 
    GTAGCATGTG CATTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCG ACGATGCAAG GACACACAGG GCGGCTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCCTTT CTGCTCCTTC ACATCATTTT CCACATCACA
    CTGGCCAGCC TGGAAGCTCA GCACCGCATC GCACCCGCTT ACAACTGCTC AACTTGGGAA
    AAGACCTTCC GGCAGATCGG CTTCGAAAGC TTGAAGGGAG CTGATGCTGG CAATGGCATC
    AGGGTGTTTG TGCCTGATAT CGGGATGTTC ATTGCGAGCC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACGGAA GAAGTAGCCC AATTTAATTC CGAGTGCGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GACTCAGAAG AAGCGCTGAT TTATGAGGAG
    GACTTAGATG GGGAAGAAGG CATGGAAGGC GAGCTGGAAG AAAGCACAAA GTTAAAAATG
    TTTCGCAGGC TCGCCTCCGT CGCTTCCAAG CTTAAGGAGT TCATCGGCAA TATGATAACC
    ACGGCGGGCA AGGTGGTGGT GACCATCCTG TTGGGCTCCT CTGGCATGAT GCTGCCATCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT CTGGGTCTGT GCACCTGGTG GTCCTGGTGC
    CGAACTTTTG ACCCATTGCT GTTTGGCTGC CTCTGTGTCC TGTTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTTCAGTTCT TCCAAGAAGC AGTCCCACCC
    AATGACTACT ATGCAAGGTT GTTTGGAATC AAATCCGTTA TTCAAACAGA CTGCTCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTACAC GCTGGCCACG CTGATCCGCA TTTGGTTGCA AGAGCCTCTT
    GTGCAGGAGG AGATGTCGAA GGAAGAGGAG GGTGCACTGG ATGGCTGCTC CAATCAGAAC
    ACAGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    CTTTTATCCA TGACCCAAGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACTGTGAAC
    GGCAACCCTG TGGACTACCA CACCATCCAC CCAAGTCTGC CCATAGAGAA TGGCCCCGCC
    AAGACGGACT TGTATACTAC TCCTCAGTAC CGGTGGGAGC CCTCAGAGGA GTCCTCAGAA
    AAGAAAGAGG AGGAAGAGGA CAAGAGAGAA GACTCAGAAG GGGAAGGGAG CCAAGAGGAA
    AAACGGAGTG TGAGAATTCA TGCCATGGTC GCCGTGTTCC AGTTCATCAT GAAGCAGAGT
    TACATCTGTG CCCTCATCGC CATGATGGCA TGGAGCATCA CCTACCACAG CTGGCTGACA
    TTTGTGCTGT TGATCTGGTC GTGCACTCTC TGGATGATTC GCAACAGGAG GAAGTACGCC
    ATGATCAGCT CTCCGTTCAT GGTTGTCTAT GCCAATCTGC TGCTGGTACT GCAGTACATA
    TGGAGCTTTG AACTTCCAGA AATCAAGAAA GTCCCTGGAT TTCTAGAAAA GAAAGAGCCT
    GGAGAGCTTG CCTCAAAGAT CCTCTTCACC ATCACATTTT GGCTGCTCCT GAGGCAGCAT
    CTCACAGAGC AGAAGGCCCT TCAGGAAAAG GAAGCACTTC TGTCTGAAGT CAAAATTGGA
    AGTCAAGAAC TTGACGAAAA GGATGAGGAA CTCCAAGATA TACAAGTGGA AGGAGAGCCC
    ATAGAGAAGG AAGAAGAGGA GGAGGAAATA AAGGAAGACA GACATGAGGT AAAGAAGGAA
    GAGGAAGAGG AAGAGGAAGA TGACGATGAC CAGGACATCA TGAAGGTTCT GGGCAATCTG
    GTGGTGGCCC TCTTCATCAA GTATTGGATC TACGTCTGCG GGGGCATGTT CTTTTTTGTC
    AGCTTTGAGG GGAAAATCGT CATGTACAAA ATCATCTACA TGGTGCTGTT CCTGTTCTGT
    GTGGCCTTGT ACCAGGTGCA CTACGAGTGG TGGAGGAAGA TCCTGAAATA CTTCTGGATG
    TCGGTGGTCA TTTACACCAT GCTGGTGCTC ATCTTCATCT ACACTTACCA GTTTGAGAAC
    TTCCCAGGCC TGTGGCAAAA CATGACCGGG TTAAAGAAAG AGAAGCTTGA GGATCTGGGC
    CTGAAGCAGT TCACTGTGGC CGAACTGTTC ACACGAATAT TCATCCCCAC CTCCTTTCTG
    CTGGTCTGCA TCCTGCACCT CCACTATTTC CATGACCGGT TCCTGGAACT CACAGACCTC
    AAGTCCATCC CCAGCAAAGA AGACAACACC ATCTACAGCC ATGCCAAGGT CAATGGTCGC
    GTGTACCTAA TAATAAATAG GTTAGCCCAC CCTGAAGGAA GCCTCCCAGA CCTAGCCATT
    ATGAACATGA CGGCCAGCCT CGATAAGCCT GAGAGGCAGA AGTTGGCAGA GCCTGGGGAG
    GAGAGGACAG AAGAATGTGC CAAGAAGGCA GAGAAGGGTG AGGCTGGGAA AGACAGTGAT
    GAGTCTGAGG AGGAGGAAGG TGAGGAAGAG GAATCCGAGG AGGAAGAATC CTCAGATCTC
    AGGAACAAGT GGCACCTCGT GATTGACCGG CTCACTGTGC TCTTTCTAAA GTTCCTGGAG
    TATTTCCACA AGTTGCAGGT GTTTATGTGG TGGATCTTGG AGCTGCACAT CATCAAAATC
    GTCTCATCGT ACATTATCTG GGTGACTGTC AAAGAGGTGT CTCTGTTCAA CTATGTATTT
    TTGATTTCTT GGGCATTTGC TCTGCCATAT GCCAAACTCC GCCGTGTGGC TTCCAGTGTC
    TGCACTGTCT GGACGTGTGT GATCATCGTC TGCAAAATGT TGTACCAGCT GCAAACCATT
    AAGCCTGAGA ACTTTTCTGT CAACTGCTCC TTGCCCAATG AAAATCAAAC CAACATACCC
    CTTAATGAGT TGAACAAGTC TCTTCTCTAC AGTGCCCCTG TTGATCCCAC TGAGTGGGTT
    GGCCTGAGGA AGTCCTCCCC TTTACTCGTC TACCTGAGGA ACAACCTCCT GATGTTGGCG
    ATTTTGGCCT TCGAAGTCAC TGTTTACCGA CATCAGGAGT ACTATCGAGG CCGAAACAAC
    TTGACTGCTC CGGTGTCTAA AACTATCTTT CATGACATCA CAAGGTTACA TCTAGATGAT
    GGGCTTATCA ATTGTGCCAA ATATTTTGTA AACTACTTCT TCTACAAGTT TGGCCTAGAG
    ACCTGTTTCC TAATGTCAGT GAACGTAATT GGACAGCGAA TGGACTTCTA CGCCATGATT
    CATGCCTGTT GGCTGATCGG TGTCTTGTAC CGTCGCAGAA GAAAAGCCAT TGCTGAGGTC
    TGGCCCAAGT ACTGCTGCTT CCTGGCTTGC ATCATCACCT TCCAGTACTT TGTCTGCATA
    GGCATCCCTC CTGCCCCCTG CCGAGATTAC CCATGGAGGT TCAAGGGTGC CAACTTCAAT
    GACAACATCA TCAAGTGGCT GTACTTCCCA GATTTCATCG TGCGGCCCAA CCCTGTGTTT
    CTTGTCTATG ACTTCATGCT GCTCCTGTGT GCTTCCTTAC AACGACAGAT TTTTGAGGAC
    GAAAACAAGG CAGCTGTGCG CATCATGGCC GGGGACAATG TCGAGATTTG CATGAACCTT
    GACGCGGCCT CATTCAGCCA GCATAACCCT GTACCAGATT TCATTCACTG CAGATCTTAC
    TTGGACATGT CCAAAGTGAT CATCTTCAGC TACCTCTTCT GGTTCGTCCT CACCATCATC
    TTCATTACCG GAACGACCAG GATCAGCATC TTTTGCATGG GTTACCTGGT GGCCTGTTTC
    TATTTCCTGC TCTTTGGAGG TGATCTGCTC CTGAAACCCA TCAAGAGCAT CCTACGCTAC
    TGGGACTGGC TGATTGCATA CAACGTGTTT GTGATCACCA TGAAAAACAT ACTGTCAATA
    GGAGCCTGTG GATACATCGG TGCACTGGTG AGAAACAGCT GCTGGCTGAT CCAAGCGTTC
    AGCCTGGCCT GCACGGTGAA AGGCTATAAA ATGCCTGAAG ATGACTCCAG ATGCAAGCTG
    CCCAGTGGGG AGGCAGGGAT CATCTGGGAC AGCATCTGCT TTGCGTTTCT CCTGCTACAG
    AGGAGAGTCT TCATGAGTTA CTATTTCCTG CATGTCGTGG CTGACATTAA AGCATCCCAG
    ATCCTGGCAT CCAGAGGGGC CGAACTTTTC CAGGCTACAA TTGTGAAAGC TGTAAAGGCA
    AGAATTGAAG AAGAGAAAAA ATCCATGGAC CAGCTGAAGA GACAGATGGA TCGCATCAAA
    GCCCGGCAGC AAAAGTACAA AAAGGGTAAG GAGAGGATGC TGAGCTTGAC CCAGGAGTCA
    GGGGAAGGCC AGGACATCCA GAAAGTCTCA GAAGAAGACG ATGAAAGAGA AGCAGACAAA
    CAGAAAGCCA AGGGCAAAAA AAAGCAGTGG TGGCGGCCTT GGGTTGATCA TGCTTCCATG
    GTCAGGAGTG GAGATTATTA TTTGTTTGAA ACGGATAGTG AAGAGGAGGA AGAAGAGGAA
    CTCAAAAAGG AGGATGAAGA ACCACCACGG AAGTCTGCAT TCCAGAGAGC TATTGGGAAG
    TTTGCCTCAG CCATTTTAGC CTTGCCAAAG TCTGTCATTA AACTTCCCAA AACTATTCTT
    CAGTATTTAA TCAGAGCTGC AAAGTTCGTC TACCAAGCCT GGATTACCGA TCCTAAAACA
    GCACTCCGAC AGAGGAGGAA AGAGAAGAAG AAAATGGCCA GAGAAGAGCA GAAGGGAAGA
    CGCAAAGGAT CTGGGGAGGG TCCAGTGGAA TGGGAAGACA GAGAAGATGA GCCAATCAAA
    AAGAAATCTG ATGGACCAGA TAATATCATC AAGAGGATAT TTAATATCCT GAAGTTCACC
    TGGGTCCTGT TCCTGGCCAC AGTGGACAGT TTCACTACTT GGCTCAACTC CATCTCAAGA
    GAGCATATCG ACATATCCAC AGTTCTGCGG ATTGAAAGAT GCATGCTGAC CCGAGAGATA
    AAGAAGGGCA ACGTTCCGAC ACGGGAAAGC ATCCACATGT ACTACCAGAA CCACATGATG
    AACCTTTCCA GAGAATCCGG ACTGGACACA ATCGACGAGC ACTCTGGCGC TGGTTCCAGG
    GCCCAGGCAG CACACAGGAT GGATAGCTTA GATTCCCATG ACAGTATCTC CAGCTGTTAC
    ACTGAAGCAA CCCTGCTGAT CTCAAGGCAG TCCACCCTTG ATGATTTAGA TGGACAAGAC
    CCTGTTCCTA AAACAAGCGA GCGTGCTCGG CCTAGGCTCC GTAAAGTGTT CAGCATGGAC
    ATGTCCTCCT CATCAGCTGA CAGTGGCAGT GTAGCATCAA GCGAGCCCAC ACAGTGCACC
    ATGCTGTATT CACGCCAAGG AACTACGGAA ACCATTGAGG AGGTAGAGGC TGAAGCTGAG
    GAGGAGGTGG TAGAGAGTCT GGAGCCTGAG CTCCATGATG CCGAAGAGAA GGAATATGCA
    GCTGACTATG AAGCAGGGGT AGAGGAGGTC AGCCTCACTC CAGATGAGGA GTTGCCTCAG
    TTCTCAACAG ATGACGGTGA CGTGGAGGCC CCGCCCTCCT ACAGCAAGGC TGTCAGCTTT
    GAGCACCTCT CGTTTGCATC ACAAGATGAC TCCGGGGGCA AGAACCACAT GGTGGTCAGC
    CCTGACGACA GCCGCACAGA CAAGCTGGAG TCCAGTATCT TACCGCCCCT GACTCACGAG
    CTGACAGCCA GCGATCTGCT CATGAGCAAG ATGTTCCGTG ATGATGAGCT TGAAGAATCT
    GAGAAATTCT ACGTGGACCA GCCCCGGTTC TTGCTGCTAT TCTATGCCAT GTATAACACT
    CTGGTAGCCC GCTCGGAAAT GGTGTGCTAT TTCGTGATCA TCCTGAACCA CATGATCTCT
    GCCTCCATCA TTACTCTTCT GTTGCCCATC CTGATCTTCC TCTGGGCCAT GCTGTCCGTC
    CCCAGGCCCA GCCGGCGCTT CTGGATGATG GCCATCGTCT ACACTGAGGT GGCGATTGTA
    GTGAAATATT TCTTCCAGTT TGGCTTCTTT CCTTGGAATA AGGACTTGGA AATTTACAAG
    GACAGGCCCT ACTTTCCACC AAACATCATA GGGGTGGAAA AGAAAGAGGG CTATGTTCTT
    TACGACCTCA TTCAGCTGCT GGCTCTGTTC TTCCATCGAT CCATTCTGAA GTGTCATGGC
    CTATGGGACG AGGATGACAT TGTTGACAGT AACACAGACA AGGAAGGATC TGATGATGAG
    CTGTCCCTCG GTCAGGGCAG AAGAGGCTCC TCAGAGTCAC TGAAGTCCAT CAACTTGGCT
    GCCTCTGTAG AGTCCGTCCA TGTGACCTTC CCAGAGCAGC CAGCAGCCAT CCGGAGAAAA
    CGCTCCTGCA GCAGCTCGCA GATCTCCCCA CGGTCCAGCT TCTCTTCAAA TAGGTCCAAA
    AGAGGCAGCA CAAGCACCCG AAACAGCAGC CAAAAAGGAA GCAGTGTTTT GAGCATTAAA
    CAAAAAAGCA AAAGAGAACT TTACATGGAA AAGCTTCAAG AACATTTAAT CAAAGCAAAG
    GCCTTTACCA TAAAGAAAAC TCTACAAATC TATGTACCCA TTCGGCAATT CTTCTATGAC
    CTCATCCACC CAGACTACAG TGCTGTGACT GACGTGTATG TGCTGATGTT CCTGGCCGAC
    ACCGTGGACT TCATCATCAT CGTCTTTGGC TTCTGGGCTT TTGGGAAACA CTCGGCAGCT
    GCAGATATCA CCTCTTCCCT ATCAGAAGAC CAGGTCCCCG GGCCATTCCT GGTGATGGTT
    CTCATTCAGT TTGGAACCAT GGTGGTGGAC CGTGCACTCT ACCTCAGGAA GACTGTGCTT
    GGCAAGGTCA TTTTCCAGGT CATCCTCGTG TTTGGAATTC ACTTCTGGAT GTTCTTCATC
    CTACCCAGTG TGACTGAGAG GAAATTCAGC CAGAACCTGG TGGCACAGCT CTGGTACTTT
    GTGAAATGTG TGTACTTTGG GTTGTCTGCC TACCAAATCC GCTGTGGCTA CCCAACACGT
    GTGCTCGGAA ACTTCCTCAC GAAGAGCTAC AATTATGTCA ACCTCTTCTT GTTTCAAGGG
    TTTCGTCTCG TCCCATTTCT GACAGAGCTG CGGGCTGTGA TGGACTGGGT GTGGACAGAT
    ACAACCTTGA GCCTCTCCAG CTGGATTTGT GTGGAGGACA TTTACGCACA CATTTTCATC
    TTAAAGTGCT GGCGAGAGTC GGAAAAGAGA TACCCTCAAC CCAGGGGCCA GAAGAAGAAG
    AAAGCTGTGA AGTATGGCAT GGGCGGAATG ATCATTGTCC TGCTCATCTG TATCGTCTGG
    TTTCCTCTGC TCTTCATGTC TTTGATCAAG TCTGTCGCAG GGGTTATTAA CCAACCCCTG
    GATGTGTCCG TCACAATAAC CCTGGGAGGC TATCAGCCTA TCTTCACAAT GAGTGCCCAG
    CAAAGCCAGC TGAAAGTTAT GGATGGTACA AAGTATAACG AATTCATAAA GTCTTTCGGC
    CCCAACAGTG GCGCTATGCA ATTTCTGGAG AATTATGAAA GGCAAGACAT AACAGTAGCG
    GAACTGGAAG GGAATTCGAA TTCTTTGTGG ACCATCAGCC CTCCCAGTAA GCAGAAAATG
    ATACAGGAGC TCACCGACCC CAATAGTTGC TTCTCTGTTG TTTTTTCATG GAGTATCCAG
    AGAAACATGA CTCTGGGTGC AAAGGCTGAA ATAGCAACGG ACAAGCTTTC TTTTCCTCTC
    ACGGATGCGA CACGAAGTAG CATAGCCAAA ATGATTGCTG GCAATGACAC AGAAAGTTCC
    AACACACCAG TGACAATAGA AAAGATCTAC CCATACTATG TGAAGGCACC CAGTGACTCA
    AACTCAAAGC CTATAAAACA GCTTCTGTCA GAAAACAATT TCATGAATAT CACCATCATT
    TTGTTCAGAG ACAATGTCAC AAAGTCCAAT AGTGAGTGGT GGGTTCTCAA CCTGACTGGA
    AGTAGGATAT TCAATCAGGG ATCCCAAGCC TTGGAGCTGG TGGTCTTCAA TGACAAAGTC
    AGCCCCCCAA GTCTGGGCTT CTTGGCTGGC TATGGCATCA TGGGATTGTA TGCATCCGTT
    GTCCTTGTAA TTGGGAAGTT TGTTCGCGAA TTCTTCAGTG GGATTTCTCA CTCTATCATG
    TTTGAAGAGC TTCCAAATGT GGACAGAATT TTGAAGTTAT GCACAGATAT ATTTCTCGTA
    CGGGAGACAG GGGAACTGGA GCTGGAGGAA GACCTCTATG CCAAATTGAT ATTCCTGTAC
    AGGTCGCCAG AAACCATGAT CAAGTGGACT AGAGAAAAAA CAAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017456604.1
    CDS940..9507
    Translation

    Target ORF information:

    RefSeq Version XM_017601115.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017601115.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTCCCCAT CTGCCTGGCA 
    GTAGCATGTG CATTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCG ACGATGCAAG GACACACAGG GCGGCTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCCTTT CTGCTCCTTC ACATCATTTT CCACATCACA
    CTGGCCAGCC TGGAAGCTCA GCACCGCATC GCACCCGCTT ACAACTGCTC AACTTGGGAA
    AAGACCTTCC GGCAGATCGG CTTCGAAAGC TTGAAGGGAG CTGATGCTGG CAATGGCATC
    AGGGTGTTTG TGCCTGATAT CGGGATGTTC ATTGCGAGCC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACGGAA GAAGTAGCCC AATTTAATTC CGAGTGCGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GACTCAGAAG AAGCGCTGAT TTATGAGGAG
    GACTTAGATG GGGAAGAAGG CATGGAAGGC GAGCTGGAAG AAAGCACAAA GTTAAAAATG
    TTTCGCAGGC TCGCCTCCGT CGCTTCCAAG CTTAAGGAGT TCATCGGCAA TATGATAACC
    ACGGCGGGCA AGGTGGTGGT GACCATCCTG TTGGGCTCCT CTGGCATGAT GCTGCCATCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT CTGGGTCTGT GCACCTGGTG GTCCTGGTGC
    CGAACTTTTG ACCCATTGCT GTTTGGCTGC CTCTGTGTCC TGTTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTTCAGTTCT TCCAAGAAGC AGTCCCACCC
    AATGACTACT ATGCAAGGTT GTTTGGAATC AAATCCGTTA TTCAAACAGA CTGCTCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTACAC GCTGGCCACG CTGATCCGCA TTTGGTTGCA AGAGCCTCTT
    GTGCAGGAGG AGATGTCGAA GGAAGAGGAG GGTGCACTGG ATGGCTGCTC CAATCAGAAC
    ACAGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    CTTTTATCCA TGACCCAAGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACTGTGAAC
    GGCAACCCTG TGGACTACCA CACCATCCAC CCAAGTCTGC CCATAGAGAA TGGCCCCGCC
    AAGACGGACT TGTATACTAC TCCTCAGTAC CGGTGGGAGC CCTCAGAGGA GTCCTCAGAA
    AAGAAAGAGG AGGAAGAGGA CAAGAGAGAA GACTCAGAAG GGGAAGGGAG CCAAGAGGAA
    AAACGGAGTG TGAGAATTCA TGCCATGGTC GCCGTGTTCC AGTTCATCAT GAAGCAGAGT
    TACATCTGTG CCCTCATCGC CATGATGGCA TGGAGCATCA CCTACCACAG CTGGCTGACA
    TTTGTGCTGT TGATCTGGTC GTGCACTCTC TGGATGATTC GCAACAGGAG GAAGTACGCC
    ATGATCAGCT CTCCGTTCAT GGTTGTCTAT GCCAATCTGC TGCTGGTACT GCAGTACATA
    TGGAGCTTTG AACTTCCAGA AATCAAGAAA GTCCCTGGAT TTCTAGAAAA GAAAGAGCCT
    GGAGAGCTTG CCTCAAAGAT CCTCTTCACC ATCACATTTT GGCTGCTCCT GAGGCAGCAT
    CTCACAGAGC AGAAGGCCCT TCAGGAAAAG GAAGCACTTC TGTCTGAAGT CAAAATTGGA
    AGTCAAGAAC TTGACGAAAA GGATGAGGAA CTCCAAGATA TACAAGTGGA AGGAGAGCCC
    ATAGAGAAGG AAGAAGAGGA GGAGGAAATA AAGGAAGACA GACATGAGGT AAAGAAGGAA
    GAGGAAGAGG AAGAGGAAGA TGACGATGAC CAGGACATCA TGAAGGTTCT GGGCAATCTG
    GTGGTGGCCC TCTTCATCAA GTATTGGATC TACGTCTGCG GGGGCATGTT CTTTTTTGTC
    AGCTTTGAGG GGAAAATCGT CATGTACAAA ATCATCTACA TGGTGCTGTT CCTGTTCTGT
    GTGGCCTTGT ACCAGGTGCA CTACGAGTGG TGGAGGAAGA TCCTGAAATA CTTCTGGATG
    TCGGTGGTCA TTTACACCAT GCTGGTGCTC ATCTTCATCT ACACTTACCA GTTTGAGAAC
    TTCCCAGGCC TGTGGCAAAA CATGACCGGG TTAAAGAAAG AGAAGCTTGA GGATCTGGGC
    CTGAAGCAGT TCACTGTGGC CGAACTGTTC ACACGAATAT TCATCCCCAC CTCCTTTCTG
    CTGGTCTGCA TCCTGCACCT CCACTATTTC CATGACCGGT TCCTGGAACT CACAGACCTC
    AAGTCCATCC CCAGCAAAGA AGACAACACC ATCTACAGCC ATGCCAAGGT CAATGGTCGC
    GTGTACCTAA TAATAAATAG GTTAGCCCAC CCTGAAGGAA GCCTCCCAGA CCTAGCCATT
    ATGAACATGA CGGCCAGCCT CGATAAGCCT GAGAGGCAGA AGTTGGCAGA GCCTGGGGAG
    GAGAGGACAG AAGAATGTGC CAAGAAGGCA GAGAAGGGTG AGGCTGGGAA AGACAGTGAT
    GAGTCTGAGG AGGAGGAAGG TGAGGAAGAG GAATCCGAGG AGGAAGAATC CTCAGATCTC
    AGGAACAAGT GGCACCTCGT GATTGACCGG CTCACTGTGC TCTTTCTAAA GTTCCTGGAG
    TATTTCCACA AGTTGCAGGT GTTTATGTGG TGGATCTTGG AGCTGCACAT CATCAAAATC
    GTCTCATCGT ACATTATCTG GGTGACTGTC AAAGAGGTGT CTCTGTTCAA CTATGTATTT
    TTGATTTCTT GGGCATTTGC TCTGCCATAT GCCAAACTCC GCCGTGTGGC TTCCAGTGTC
    TGCACTGTCT GGACGTGTGT GATCATCGTC TGCAAAATGT TGTACCAGCT GCAAACCATT
    AAGCCTGAGA ACTTTTCTGT CAACTGCTCC TTGCCCAATG AAAATCAAAC CAACATACCC
    CTTAATGAGT TGAACAAGTC TCTTCTCTAC AGTGCCCCTG TTGATCCCAC TGAGTGGGTT
    GGCCTGAGGA AGTCCTCCCC TTTACTCGTC TACCTGAGGA ACAACCTCCT GATGTTGGCG
    ATTTTGGCCT TCGAAGTCAC TGTTTACCGA CATCAGGAGT ACTATCGAGG CCGAAACAAC
    TTGACTGCTC CGGTGTCTAA AACTATCTTT CATGACATCA CAAGGTTACA TCTAGATGAT
    GGGCTTATCA ATTGTGCCAA ATATTTTGTA AACTACTTCT TCTACAAGTT TGGCCTAGAG
    ACCTGTTTCC TAATGTCAGT GAACGTAATT GGACAGCGAA TGGACTTCTA CGCCATGATT
    CATGCCTGTT GGCTGATCGG TGTCTTGTAC CGTCGCAGAA GAAAAGCCAT TGCTGAGGTC
    TGGCCCAAGT ACTGCTGCTT CCTGGCTTGC ATCATCACCT TCCAGTACTT TGTCTGCATA
    GGCATCCCTC CTGCCCCCTG CCGAGATTAC CCATGGAGGT TCAAGGGTGC CAACTTCAAT
    GACAACATCA TCAAGTGGCT GTACTTCCCA GATTTCATCG TGCGGCCCAA CCCTGTGTTT
    CTTGTCTATG ACTTCATGCT GCTCCTGTGT GCTTCCTTAC AACGACAGAT TTTTGAGGAC
    GAAAACAAGG CAGCTGTGCG CATCATGGCC GGGGACAATG TCGAGATTTG CATGAACCTT
    GACGCGGCCT CATTCAGCCA GCATAACCCT GTACCAGATT TCATTCACTG CAGATCTTAC
    TTGGACATGT CCAAAGTGAT CATCTTCAGC TACCTCTTCT GGTTCGTCCT CACCATCATC
    TTCATTACCG GAACGACCAG GATCAGCATC TTTTGCATGG GTTACCTGGT GGCCTGTTTC
    TATTTCCTGC TCTTTGGAGG TGATCTGCTC CTGAAACCCA TCAAGAGCAT CCTACGCTAC
    TGGGACTGGC TGATTGCATA CAACGTGTTT GTGATCACCA TGAAAAACAT ACTGTCAATA
    GGAGCCTGTG GATACATCGG TGCACTGGTG AGAAACAGCT GCTGGCTGAT CCAAGCGTTC
    AGCCTGGCCT GCACGGTGAA AGGCTATAAA ATGCCTGAAG ATGACTCCAG ATGCAAGCTG
    CCCAGTGGGG AGGCAGGGAT CATCTGGGAC AGCATCTGCT TTGCGTTTCT CCTGCTACAG
    AGGAGAGTCT TCATGAGTTA CTATTTCCTG CATGTCGTGG CTGACATTAA AGCATCCCAG
    ATCCTGGCAT CCAGAGGGGC CGAACTTTTC CAGGCTACAA TTGTGAAAGC TGTAAAGGCA
    AGAATTGAAG AAGAGAAAAA ATCCATGGAC CAGCTGAAGA GACAGATGGA TCGCATCAAA
    GCCCGGCAGC AAAAGTACAA AAAGGGTAAG GAGAGGATGC TGAGCTTGAC CCAGGAGTCA
    GGGGAAGGCC AGGACATCCA GAAAGTCTCA GAAGAAGACG ATGAAAGAGA AGCAGACAAA
    CAGAAAGCCA AGGGCAAAAA AAAGCAGTGG TGGCGGCCTT GGGTTGATCA TGCTTCCATG
    GTCAGGAGTG GAGATTATTA TTTGTTTGAA ACGGATAGTG AAGAGGAGGA AGAAGAGGAA
    CTCAAAAAGG AGGATGAAGA ACCACCACGG AAGTCTGCAT TCCAGAGAGC TATTGGGAAG
    TTTGCCTCAG CCATTTTAGC CTTGCCAAAG TCTGTCATTA AACTTCCCAA AACTATTCTT
    CAGTATTTAA TCAGAGCTGC AAAGTTCGTC TACCAAGCCT GGATTACCGA TCCTAAAACA
    GCACTCCGAC AGAGGAGGAA AGAGAAGAAG AAAATGGCCA GAGAAGAGCA GAAGGGAAGA
    CGCAAAGGAT CTGGGGAGGG TCCAGTGGAA TGGGAAGACA GAGAAGATGA GCCAATCAAA
    AAGAAATCTG ATGGACCAGA TAATATCATC AAGAGGATAT TTAATATCCT GAAGTTCACC
    TGGGTCCTGT TCCTGGCCAC AGTGGACAGT TTCACTACTT GGCTCAACTC CATCTCAAGA
    GAGCATATCG ACATATCCAC AGTTCTGCGG ATTGAAAGAT GCATGCTGAC CCGAGAGATA
    AAGAAGGGCA ACGTTCCGAC ACGGGAAAGC ATCCACATGT ACTACCAGAA CCACATGATG
    AACCTTTCCA GAGAATCCGG ACTGGACACA ATCGACGAGC ACTCTGGCGC TGGTTCCAGG
    GCCCAGGCAG CACACAGGAT GGATAGCTTA GATTCCCATG ACAGTATCTC CAGCTGTTAC
    ACTGAAGCAA CCCTGCTGAT CTCAAGGCAG TCCACCCTTG ATGATTTAGA TGGACAAGAC
    CCTGTTCCTA AAACAAGCGA GCGTGCTCGG CCTAGGCTCC GTAAAGTGTT CAGCATGGAC
    ATGTCCTCCT CATCAGCTGA CAGTGGCAGT GTAGCATCAA GCGAGCCCAC ACAGTGCACC
    ATGCTGTATT CACGCCAAGG AACTACGGAA ACCATTGAGG AGGTAGAGGC TGAAGCTGAG
    GAGGAGGTGG TAGAGAGTCT GGAGCCTGAG CTCCATGATG CCGAAGAGAA GGAATATGCA
    GCTGACTATG AAGCAGGGGT AGAGGAGGTC AGCCTCACTC CAGATGAGGA GTTGCCTCAG
    TTCTCAACAG ATGACGGTGA CGTGGAGGCC CCGCCCTCCT ACAGCAAGGC TGTCAGCTTT
    GAGCACCTCT CGTTTGCATC ACAAGATGAC TCCGGGGGCA AGAACCACAT GGTGGTCAGC
    CCTGACGACA GCCGCACAGA CAAGCTGGAG TCCAGTATCT TACCGCCCCT GACTCACGAG
    CTGACAGCCA GCGATCTGCT CATGAGCAAG ATGTTCCGTG ATGATGAGCT TGAAGAATCT
    GAGAAATTCT ACGTGGACCA GCCCCGGTTC TTGCTGCTAT TCTATGCCAT GTATAACACT
    CTGGTAGCCC GCTCGGAAAT GGTGTGCTAT TTCGTGATCA TCCTGAACCA CATGATCTCT
    GCCTCCATCA TTACTCTTCT GTTGCCCATC CTGATCTTCC TCTGGGCCAT GCTGTCCGTC
    CCCAGGCCCA GCCGGCGCTT CTGGATGATG GCCATCGTCT ACACTGAGGT GGCGATTGTA
    GTGAAATATT TCTTCCAGTT TGGCTTCTTT CCTTGGAATA AGGACTTGGA AATTTACAAG
    GACAGGCCCT ACTTTCCACC AAACATCATA GGGGTGGAAA AGAAAGAGGG CTATGTTCTT
    TACGACCTCA TTCAGCTGCT GGCTCTGTTC TTCCATCGAT CCATTCTGAA GTGTCATGGC
    CTATGGGACG AGGATGACAT TGTTGACAGT AACACAGACA AGGAAGGATC TGATGATGAG
    CTGTCCCTCG GTCAGGGCAG AAGAGGCTCC TCAGAGTCAC TGAAGTCCAT CAACTTGGCT
    GCCTCTGTAG AGTCCGTCCA TGTGACCTTC CCAGAGCAGC CAGCAGCCAT CCGGAGAAAA
    CGCTCCTGCA GCAGCTCGCA GATCTCCCCA CGGTCCAGCT TCTCTTCAAA TAGGTCCAAA
    AGAGGCAGCA CAAGCACCCG AAACAGCAGC CAAAAAGGAA GCAGTGTTTT GAGCATTAAA
    CAAAAAAGCA AAAGAGAACT TTACATGGAA AAGCTTCAAG AACATTTAAT CAAAGCAAAG
    GCCTTTACCA TAAAGAAAAC TCTACAAATC TATGTACCCA TTCGGCAATT CTTCTATGAC
    CTCATCCACC CAGACTACAG TGCTGTGACT GACGTGTATG TGCTGATGTT CCTGGCCGAC
    ACCGTGGACT TCATCATCAT CGTCTTTGGC TTCTGGGCTT TTGGGAAACA CTCGGCAGCT
    GCAGATATCA CCTCTTCCCT ATCAGAAGAC CAGGTCCCCG GGCCATTCCT GGTGATGGTT
    CTCATTCAGT TTGGAACCAT GGTGGTGGAC CGTGCACTCT ACCTCAGGAA GACTGTGCTT
    GGCAAGGTCA TTTTCCAGGT CATCCTCGTG TTTGGAATTC ACTTCTGGAT GTTCTTCATC
    CTACCCAGTG TGACTGAGAG GAAATTCAGC CAGAACCTGG TGGCACAGCT CTGGTACTTT
    GTGAAATGTG TGTACTTTGG GTTGTCTGCC TACCAAATCC GCTGTGGCTA CCCAACACGT
    GTGCTCGGAA ACTTCCTCAC GAAGAGCTAC AATTATGTCA ACCTCTTCTT GTTTCAAGGG
    TTTCGTCTCG TCCCATTTCT GACAGAGCTG CGGGCTGTGA TGGACTGGGT GTGGACAGAT
    ACAACCTTGA GCCTCTCCAG CTGGATTTGT GTGGAGGACA TTTACGCACA CATTTTCATC
    TTAAAGTGCT GGCGAGAGTC GGAAAAGAGA TACCCTCAAC CCAGGGGCCA GAAGAAGAAG
    AAAGCTGTGA AGTATGGCAT GGGCGGAATG ATCATTGTCC TGCTCATCTG TATCGTCTGG
    TTTCCTCTGC TCTTCATGTC TTTGATCAAG TCTGTCGCAG GGGTTATTAA CCAACCCCTG
    GATGTGTCCG TCACAATAAC CCTGGGAGGC TATCAGCCTA TCTTCACAAT GAGTGCCCAG
    CAAAGCCAGC TGAAAGTTAT GGATGGTACA AAGTATAACG AATTCATAAA GTCTTTCGGC
    CCCAACAGTG GCGCTATGCA ATTTCTGGAG AATTATGAAA GGCAAGACAT AACAGTAGCG
    GAACTGGAAG GGAATTCGAA TTCTTTGTGG ACCATCAGCC CTCCCAGTAA GCAGAAAATG
    ATACAGGAGC TCACCGACCC CAATAGTTGC TTCTCTGTTG TTTTTTCATG GAGTATCCAG
    AGAAACATGA CTCTGGGTGC AAAGGCTGAA ATAGCAACGG ACAAGCTTTC TTTTCCTCTC
    ACGGATGCGA CACGAAGTAG CATAGCCAAA ATGATTGCTG GCAATGACAC AGAAAGTTCC
    AACACACCAG TGACAATAGA AAAGATCTAC CCATACTATG TGAAGGCACC CAGTGACTCA
    AACTCAAAGC CTATAAAACA GCTTCTGTCA GAAAACAATT TCATGAATAT CACCATCATT
    TTGTTCAGAG ACAATGTCAC AAAGTCCAAT AGTGAGTGGT GGGTTCTCAA CCTGACTGGA
    AGTAGGATAT TCAATCAGGG ATCCCAAGCC TTGGAGCTGG TGGTCTTCAA TGACAAAGTC
    AGCCCCCCAA GTCTGGGCTT CTTGGCTGGC TATGGCATCA TGGGATTGTA TGCATCCGTT
    GTCCTTGTAA TTGGGAAGTT TGTTCGCGAA TTCTTCAGTG GGATTTCTCA CTCTATCATG
    TTTGAAGAGC TTCCAAATGT GGACAGAATT TTGAAGTTAT GCACAGATAT ATTTCTCGTA
    CGGGAGACAG GGGAACTGGA GCTGGAGGAA GACCTCTATG CCAAATTGAT ATTCCTGTAC
    AGGTCGCCAG AAACCATGAT CAAGTGGACT AGAGAAAAAA CAAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa54717
    Clone ID Related Accession (Same CDS sequence) XM_017601115.1 , XM_017587876.1
    Accession Version XM_017587876.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8568bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product piezo-type mechanosensitive ion channel component 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000086.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    121
    181
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    301
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    481
    541
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    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
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    1921
    1981
    2041
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    2641
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    3001
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    3181
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    4141
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    6301
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    6421
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    7741
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    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTCCCCAT CTGCCTGGCA 
    GTAGCATGTG CATTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCG ACGATGCAAG GACACACAGG GCGGCTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCCTTT CTGCTCCTTC ACATCATTTT CCACATCACA
    CTGGCCAGCC TGGAAGCTCA GCACCGCATC GCACCCGCTT ACAACTGCTC AACTTGGGAA
    AAGACCTTCC GGCAGATCGG CTTCGAAAGC TTGAAGGGAG CTGATGCTGG CAATGGCATC
    AGGGTGTTTG TGCCTGATAT CGGGATGTTC ATTGCGAGCC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACGGAA GAAGTAGCCC AATTTAATTC CGAGTGCGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GACTCAGAAG AAGCGCTGAT TTATGAGGAG
    GACTTAGATG GGGAAGAAGG CATGGAAGGC GAGCTGGAAG AAAGCACAAA GTTAAAAATG
    TTTCGCAGGC TCGCCTCCGT CGCTTCCAAG CTTAAGGAGT TCATCGGCAA TATGATAACC
    ACGGCGGGCA AGGTGGTGGT GACCATCCTG TTGGGCTCCT CTGGCATGAT GCTGCCATCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT CTGGGTCTGT GCACCTGGTG GTCCTGGTGC
    CGAACTTTTG ACCCATTGCT GTTTGGCTGC CTCTGTGTCC TGTTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTTCAGTTCT TCCAAGAAGC AGTCCCACCC
    AATGACTACT ATGCAAGGTT GTTTGGAATC AAATCCGTTA TTCAAACAGA CTGCTCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTACAC GCTGGCCACG CTGATCCGCA TTTGGTTGCA AGAGCCTCTT
    GTGCAGGAGG AGATGTCGAA GGAAGAGGAG GGTGCACTGG ATGGCTGCTC CAATCAGAAC
    ACAGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    CTTTTATCCA TGACCCAAGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACTGTGAAC
    GGCAACCCTG TGGACTACCA CACCATCCAC CCAAGTCTGC CCATAGAGAA TGGCCCCGCC
    AAGACGGACT TGTATACTAC TCCTCAGTAC CGGTGGGAGC CCTCAGAGGA GTCCTCAGAA
    AAGAAAGAGG AGGAAGAGGA CAAGAGAGAA GACTCAGAAG GGGAAGGGAG CCAAGAGGAA
    AAACGGAGTG TGAGAATTCA TGCCATGGTC GCCGTGTTCC AGTTCATCAT GAAGCAGAGT
    TACATCTGTG CCCTCATCGC CATGATGGCA TGGAGCATCA CCTACCACAG CTGGCTGACA
    TTTGTGCTGT TGATCTGGTC GTGCACTCTC TGGATGATTC GCAACAGGAG GAAGTACGCC
    ATGATCAGCT CTCCGTTCAT GGTTGTCTAT GCCAATCTGC TGCTGGTACT GCAGTACATA
    TGGAGCTTTG AACTTCCAGA AATCAAGAAA GTCCCTGGAT TTCTAGAAAA GAAAGAGCCT
    GGAGAGCTTG CCTCAAAGAT CCTCTTCACC ATCACATTTT GGCTGCTCCT GAGGCAGCAT
    CTCACAGAGC AGAAGGCCCT TCAGGAAAAG GAAGCACTTC TGTCTGAAGT CAAAATTGGA
    AGTCAAGAAC TTGACGAAAA GGATGAGGAA CTCCAAGATA TACAAGTGGA AGGAGAGCCC
    ATAGAGAAGG AAGAAGAGGA GGAGGAAATA AAGGAAGACA GACATGAGGT AAAGAAGGAA
    GAGGAAGAGG AAGAGGAAGA TGACGATGAC CAGGACATCA TGAAGGTTCT GGGCAATCTG
    GTGGTGGCCC TCTTCATCAA GTATTGGATC TACGTCTGCG GGGGCATGTT CTTTTTTGTC
    AGCTTTGAGG GGAAAATCGT CATGTACAAA ATCATCTACA TGGTGCTGTT CCTGTTCTGT
    GTGGCCTTGT ACCAGGTGCA CTACGAGTGG TGGAGGAAGA TCCTGAAATA CTTCTGGATG
    TCGGTGGTCA TTTACACCAT GCTGGTGCTC ATCTTCATCT ACACTTACCA GTTTGAGAAC
    TTCCCAGGCC TGTGGCAAAA CATGACCGGG TTAAAGAAAG AGAAGCTTGA GGATCTGGGC
    CTGAAGCAGT TCACTGTGGC CGAACTGTTC ACACGAATAT TCATCCCCAC CTCCTTTCTG
    CTGGTCTGCA TCCTGCACCT CCACTATTTC CATGACCGGT TCCTGGAACT CACAGACCTC
    AAGTCCATCC CCAGCAAAGA AGACAACACC ATCTACAGCC ATGCCAAGGT CAATGGTCGC
    GTGTACCTAA TAATAAATAG GTTAGCCCAC CCTGAAGGAA GCCTCCCAGA CCTAGCCATT
    ATGAACATGA CGGCCAGCCT CGATAAGCCT GAGAGGCAGA AGTTGGCAGA GCCTGGGGAG
    GAGAGGACAG AAGAATGTGC CAAGAAGGCA GAGAAGGGTG AGGCTGGGAA AGACAGTGAT
    GAGTCTGAGG AGGAGGAAGG TGAGGAAGAG GAATCCGAGG AGGAAGAATC CTCAGATCTC
    AGGAACAAGT GGCACCTCGT GATTGACCGG CTCACTGTGC TCTTTCTAAA GTTCCTGGAG
    TATTTCCACA AGTTGCAGGT GTTTATGTGG TGGATCTTGG AGCTGCACAT CATCAAAATC
    GTCTCATCGT ACATTATCTG GGTGACTGTC AAAGAGGTGT CTCTGTTCAA CTATGTATTT
    TTGATTTCTT GGGCATTTGC TCTGCCATAT GCCAAACTCC GCCGTGTGGC TTCCAGTGTC
    TGCACTGTCT GGACGTGTGT GATCATCGTC TGCAAAATGT TGTACCAGCT GCAAACCATT
    AAGCCTGAGA ACTTTTCTGT CAACTGCTCC TTGCCCAATG AAAATCAAAC CAACATACCC
    CTTAATGAGT TGAACAAGTC TCTTCTCTAC AGTGCCCCTG TTGATCCCAC TGAGTGGGTT
    GGCCTGAGGA AGTCCTCCCC TTTACTCGTC TACCTGAGGA ACAACCTCCT GATGTTGGCG
    ATTTTGGCCT TCGAAGTCAC TGTTTACCGA CATCAGGAGT ACTATCGAGG CCGAAACAAC
    TTGACTGCTC CGGTGTCTAA AACTATCTTT CATGACATCA CAAGGTTACA TCTAGATGAT
    GGGCTTATCA ATTGTGCCAA ATATTTTGTA AACTACTTCT TCTACAAGTT TGGCCTAGAG
    ACCTGTTTCC TAATGTCAGT GAACGTAATT GGACAGCGAA TGGACTTCTA CGCCATGATT
    CATGCCTGTT GGCTGATCGG TGTCTTGTAC CGTCGCAGAA GAAAAGCCAT TGCTGAGGTC
    TGGCCCAAGT ACTGCTGCTT CCTGGCTTGC ATCATCACCT TCCAGTACTT TGTCTGCATA
    GGCATCCCTC CTGCCCCCTG CCGAGATTAC CCATGGAGGT TCAAGGGTGC CAACTTCAAT
    GACAACATCA TCAAGTGGCT GTACTTCCCA GATTTCATCG TGCGGCCCAA CCCTGTGTTT
    CTTGTCTATG ACTTCATGCT GCTCCTGTGT GCTTCCTTAC AACGACAGAT TTTTGAGGAC
    GAAAACAAGG CAGCTGTGCG CATCATGGCC GGGGACAATG TCGAGATTTG CATGAACCTT
    GACGCGGCCT CATTCAGCCA GCATAACCCT GTACCAGATT TCATTCACTG CAGATCTTAC
    TTGGACATGT CCAAAGTGAT CATCTTCAGC TACCTCTTCT GGTTCGTCCT CACCATCATC
    TTCATTACCG GAACGACCAG GATCAGCATC TTTTGCATGG GTTACCTGGT GGCCTGTTTC
    TATTTCCTGC TCTTTGGAGG TGATCTGCTC CTGAAACCCA TCAAGAGCAT CCTACGCTAC
    TGGGACTGGC TGATTGCATA CAACGTGTTT GTGATCACCA TGAAAAACAT ACTGTCAATA
    GGAGCCTGTG GATACATCGG TGCACTGGTG AGAAACAGCT GCTGGCTGAT CCAAGCGTTC
    AGCCTGGCCT GCACGGTGAA AGGCTATAAA ATGCCTGAAG ATGACTCCAG ATGCAAGCTG
    CCCAGTGGGG AGGCAGGGAT CATCTGGGAC AGCATCTGCT TTGCGTTTCT CCTGCTACAG
    AGGAGAGTCT TCATGAGTTA CTATTTCCTG CATGTCGTGG CTGACATTAA AGCATCCCAG
    ATCCTGGCAT CCAGAGGGGC CGAACTTTTC CAGGCTACAA TTGTGAAAGC TGTAAAGGCA
    AGAATTGAAG AAGAGAAAAA ATCCATGGAC CAGCTGAAGA GACAGATGGA TCGCATCAAA
    GCCCGGCAGC AAAAGTACAA AAAGGGTAAG GAGAGGATGC TGAGCTTGAC CCAGGAGTCA
    GGGGAAGGCC AGGACATCCA GAAAGTCTCA GAAGAAGACG ATGAAAGAGA AGCAGACAAA
    CAGAAAGCCA AGGGCAAAAA AAAGCAGTGG TGGCGGCCTT GGGTTGATCA TGCTTCCATG
    GTCAGGAGTG GAGATTATTA TTTGTTTGAA ACGGATAGTG AAGAGGAGGA AGAAGAGGAA
    CTCAAAAAGG AGGATGAAGA ACCACCACGG AAGTCTGCAT TCCAGAGAGC TATTGGGAAG
    TTTGCCTCAG CCATTTTAGC CTTGCCAAAG TCTGTCATTA AACTTCCCAA AACTATTCTT
    CAGTATTTAA TCAGAGCTGC AAAGTTCGTC TACCAAGCCT GGATTACCGA TCCTAAAACA
    GCACTCCGAC AGAGGAGGAA AGAGAAGAAG AAAATGGCCA GAGAAGAGCA GAAGGGAAGA
    CGCAAAGGAT CTGGGGAGGG TCCAGTGGAA TGGGAAGACA GAGAAGATGA GCCAATCAAA
    AAGAAATCTG ATGGACCAGA TAATATCATC AAGAGGATAT TTAATATCCT GAAGTTCACC
    TGGGTCCTGT TCCTGGCCAC AGTGGACAGT TTCACTACTT GGCTCAACTC CATCTCAAGA
    GAGCATATCG ACATATCCAC AGTTCTGCGG ATTGAAAGAT GCATGCTGAC CCGAGAGATA
    AAGAAGGGCA ACGTTCCGAC ACGGGAAAGC ATCCACATGT ACTACCAGAA CCACATGATG
    AACCTTTCCA GAGAATCCGG ACTGGACACA ATCGACGAGC ACTCTGGCGC TGGTTCCAGG
    GCCCAGGCAG CACACAGGAT GGATAGCTTA GATTCCCATG ACAGTATCTC CAGCTGTTAC
    ACTGAAGCAA CCCTGCTGAT CTCAAGGCAG TCCACCCTTG ATGATTTAGA TGGACAAGAC
    CCTGTTCCTA AAACAAGCGA GCGTGCTCGG CCTAGGCTCC GTAAAGTGTT CAGCATGGAC
    ATGTCCTCCT CATCAGCTGA CAGTGGCAGT GTAGCATCAA GCGAGCCCAC ACAGTGCACC
    ATGCTGTATT CACGCCAAGG AACTACGGAA ACCATTGAGG AGGTAGAGGC TGAAGCTGAG
    GAGGAGGTGG TAGAGAGTCT GGAGCCTGAG CTCCATGATG CCGAAGAGAA GGAATATGCA
    GCTGACTATG AAGCAGGGGT AGAGGAGGTC AGCCTCACTC CAGATGAGGA GTTGCCTCAG
    TTCTCAACAG ATGACGGTGA CGTGGAGGCC CCGCCCTCCT ACAGCAAGGC TGTCAGCTTT
    GAGCACCTCT CGTTTGCATC ACAAGATGAC TCCGGGGGCA AGAACCACAT GGTGGTCAGC
    CCTGACGACA GCCGCACAGA CAAGCTGGAG TCCAGTATCT TACCGCCCCT GACTCACGAG
    CTGACAGCCA GCGATCTGCT CATGAGCAAG ATGTTCCGTG ATGATGAGCT TGAAGAATCT
    GAGAAATTCT ACGTGGACCA GCCCCGGTTC TTGCTGCTAT TCTATGCCAT GTATAACACT
    CTGGTAGCCC GCTCGGAAAT GGTGTGCTAT TTCGTGATCA TCCTGAACCA CATGATCTCT
    GCCTCCATCA TTACTCTTCT GTTGCCCATC CTGATCTTCC TCTGGGCCAT GCTGTCCGTC
    CCCAGGCCCA GCCGGCGCTT CTGGATGATG GCCATCGTCT ACACTGAGGT GGCGATTGTA
    GTGAAATATT TCTTCCAGTT TGGCTTCTTT CCTTGGAATA AGGACTTGGA AATTTACAAG
    GACAGGCCCT ACTTTCCACC AAACATCATA GGGGTGGAAA AGAAAGAGGG CTATGTTCTT
    TACGACCTCA TTCAGCTGCT GGCTCTGTTC TTCCATCGAT CCATTCTGAA GTGTCATGGC
    CTATGGGACG AGGATGACAT TGTTGACAGT AACACAGACA AGGAAGGATC TGATGATGAG
    CTGTCCCTCG GTCAGGGCAG AAGAGGCTCC TCAGAGTCAC TGAAGTCCAT CAACTTGGCT
    GCCTCTGTAG AGTCCGTCCA TGTGACCTTC CCAGAGCAGC CAGCAGCCAT CCGGAGAAAA
    CGCTCCTGCA GCAGCTCGCA GATCTCCCCA CGGTCCAGCT TCTCTTCAAA TAGGTCCAAA
    AGAGGCAGCA CAAGCACCCG AAACAGCAGC CAAAAAGGAA GCAGTGTTTT GAGCATTAAA
    CAAAAAAGCA AAAGAGAACT TTACATGGAA AAGCTTCAAG AACATTTAAT CAAAGCAAAG
    GCCTTTACCA TAAAGAAAAC TCTACAAATC TATGTACCCA TTCGGCAATT CTTCTATGAC
    CTCATCCACC CAGACTACAG TGCTGTGACT GACGTGTATG TGCTGATGTT CCTGGCCGAC
    ACCGTGGACT TCATCATCAT CGTCTTTGGC TTCTGGGCTT TTGGGAAACA CTCGGCAGCT
    GCAGATATCA CCTCTTCCCT ATCAGAAGAC CAGGTCCCCG GGCCATTCCT GGTGATGGTT
    CTCATTCAGT TTGGAACCAT GGTGGTGGAC CGTGCACTCT ACCTCAGGAA GACTGTGCTT
    GGCAAGGTCA TTTTCCAGGT CATCCTCGTG TTTGGAATTC ACTTCTGGAT GTTCTTCATC
    CTACCCAGTG TGACTGAGAG GAAATTCAGC CAGAACCTGG TGGCACAGCT CTGGTACTTT
    GTGAAATGTG TGTACTTTGG GTTGTCTGCC TACCAAATCC GCTGTGGCTA CCCAACACGT
    GTGCTCGGAA ACTTCCTCAC GAAGAGCTAC AATTATGTCA ACCTCTTCTT GTTTCAAGGG
    TTTCGTCTCG TCCCATTTCT GACAGAGCTG CGGGCTGTGA TGGACTGGGT GTGGACAGAT
    ACAACCTTGA GCCTCTCCAG CTGGATTTGT GTGGAGGACA TTTACGCACA CATTTTCATC
    TTAAAGTGCT GGCGAGAGTC GGAAAAGAGA TACCCTCAAC CCAGGGGCCA GAAGAAGAAG
    AAAGCTGTGA AGTATGGCAT GGGCGGAATG ATCATTGTCC TGCTCATCTG TATCGTCTGG
    TTTCCTCTGC TCTTCATGTC TTTGATCAAG TCTGTCGCAG GGGTTATTAA CCAACCCCTG
    GATGTGTCCG TCACAATAAC CCTGGGAGGC TATCAGCCTA TCTTCACAAT GAGTGCCCAG
    CAAAGCCAGC TGAAAGTTAT GGATGGTACA AAGTATAACG AATTCATAAA GTCTTTCGGC
    CCCAACAGTG GCGCTATGCA ATTTCTGGAG AATTATGAAA GGCAAGACAT AACAGTAGCG
    GAACTGGAAG GGAATTCGAA TTCTTTGTGG ACCATCAGCC CTCCCAGTAA GCAGAAAATG
    ATACAGGAGC TCACCGACCC CAATAGTTGC TTCTCTGTTG TTTTTTCATG GAGTATCCAG
    AGAAACATGA CTCTGGGTGC AAAGGCTGAA ATAGCAACGG ACAAGCTTTC TTTTCCTCTC
    ACGGATGCGA CACGAAGTAG CATAGCCAAA ATGATTGCTG GCAATGACAC AGAAAGTTCC
    AACACACCAG TGACAATAGA AAAGATCTAC CCATACTATG TGAAGGCACC CAGTGACTCA
    AACTCAAAGC CTATAAAACA GCTTCTGTCA GAAAACAATT TCATGAATAT CACCATCATT
    TTGTTCAGAG ACAATGTCAC AAAGTCCAAT AGTGAGTGGT GGGTTCTCAA CCTGACTGGA
    AGTAGGATAT TCAATCAGGG ATCCCAAGCC TTGGAGCTGG TGGTCTTCAA TGACAAAGTC
    AGCCCCCCAA GTCTGGGCTT CTTGGCTGGC TATGGCATCA TGGGATTGTA TGCATCCGTT
    GTCCTTGTAA TTGGGAAGTT TGTTCGCGAA TTCTTCAGTG GGATTTCTCA CTCTATCATG
    TTTGAAGAGC TTCCAAATGT GGACAGAATT TTGAAGTTAT GCACAGATAT ATTTCTCGTA
    CGGGAGACAG GGGAACTGGA GCTGGAGGAA GACCTCTATG CCAAATTGAT ATTCCTGTAC
    AGGTCGCCAG AAACCATGAT CAAGTGGACT AGAGAAAAAA CAAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017443365.1
    CDS940..9507
    Translation

    Target ORF information:

    RefSeq Version XM_017587876.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017587876.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTCCCCAT CTGCCTGGCA 
    GTAGCATGTG CATTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCG ACGATGCAAG GACACACAGG GCGGCTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCCTTT CTGCTCCTTC ACATCATTTT CCACATCACA
    CTGGCCAGCC TGGAAGCTCA GCACCGCATC GCACCCGCTT ACAACTGCTC AACTTGGGAA
    AAGACCTTCC GGCAGATCGG CTTCGAAAGC TTGAAGGGAG CTGATGCTGG CAATGGCATC
    AGGGTGTTTG TGCCTGATAT CGGGATGTTC ATTGCGAGCC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACGGAA GAAGTAGCCC AATTTAATTC CGAGTGCGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GACTCAGAAG AAGCGCTGAT TTATGAGGAG
    GACTTAGATG GGGAAGAAGG CATGGAAGGC GAGCTGGAAG AAAGCACAAA GTTAAAAATG
    TTTCGCAGGC TCGCCTCCGT CGCTTCCAAG CTTAAGGAGT TCATCGGCAA TATGATAACC
    ACGGCGGGCA AGGTGGTGGT GACCATCCTG TTGGGCTCCT CTGGCATGAT GCTGCCATCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT CTGGGTCTGT GCACCTGGTG GTCCTGGTGC
    CGAACTTTTG ACCCATTGCT GTTTGGCTGC CTCTGTGTCC TGTTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTTCAGTTCT TCCAAGAAGC AGTCCCACCC
    AATGACTACT ATGCAAGGTT GTTTGGAATC AAATCCGTTA TTCAAACAGA CTGCTCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTACAC GCTGGCCACG CTGATCCGCA TTTGGTTGCA AGAGCCTCTT
    GTGCAGGAGG AGATGTCGAA GGAAGAGGAG GGTGCACTGG ATGGCTGCTC CAATCAGAAC
    ACAGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    CTTTTATCCA TGACCCAAGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACTGTGAAC
    GGCAACCCTG TGGACTACCA CACCATCCAC CCAAGTCTGC CCATAGAGAA TGGCCCCGCC
    AAGACGGACT TGTATACTAC TCCTCAGTAC CGGTGGGAGC CCTCAGAGGA GTCCTCAGAA
    AAGAAAGAGG AGGAAGAGGA CAAGAGAGAA GACTCAGAAG GGGAAGGGAG CCAAGAGGAA
    AAACGGAGTG TGAGAATTCA TGCCATGGTC GCCGTGTTCC AGTTCATCAT GAAGCAGAGT
    TACATCTGTG CCCTCATCGC CATGATGGCA TGGAGCATCA CCTACCACAG CTGGCTGACA
    TTTGTGCTGT TGATCTGGTC GTGCACTCTC TGGATGATTC GCAACAGGAG GAAGTACGCC
    ATGATCAGCT CTCCGTTCAT GGTTGTCTAT GCCAATCTGC TGCTGGTACT GCAGTACATA
    TGGAGCTTTG AACTTCCAGA AATCAAGAAA GTCCCTGGAT TTCTAGAAAA GAAAGAGCCT
    GGAGAGCTTG CCTCAAAGAT CCTCTTCACC ATCACATTTT GGCTGCTCCT GAGGCAGCAT
    CTCACAGAGC AGAAGGCCCT TCAGGAAAAG GAAGCACTTC TGTCTGAAGT CAAAATTGGA
    AGTCAAGAAC TTGACGAAAA GGATGAGGAA CTCCAAGATA TACAAGTGGA AGGAGAGCCC
    ATAGAGAAGG AAGAAGAGGA GGAGGAAATA AAGGAAGACA GACATGAGGT AAAGAAGGAA
    GAGGAAGAGG AAGAGGAAGA TGACGATGAC CAGGACATCA TGAAGGTTCT GGGCAATCTG
    GTGGTGGCCC TCTTCATCAA GTATTGGATC TACGTCTGCG GGGGCATGTT CTTTTTTGTC
    AGCTTTGAGG GGAAAATCGT CATGTACAAA ATCATCTACA TGGTGCTGTT CCTGTTCTGT
    GTGGCCTTGT ACCAGGTGCA CTACGAGTGG TGGAGGAAGA TCCTGAAATA CTTCTGGATG
    TCGGTGGTCA TTTACACCAT GCTGGTGCTC ATCTTCATCT ACACTTACCA GTTTGAGAAC
    TTCCCAGGCC TGTGGCAAAA CATGACCGGG TTAAAGAAAG AGAAGCTTGA GGATCTGGGC
    CTGAAGCAGT TCACTGTGGC CGAACTGTTC ACACGAATAT TCATCCCCAC CTCCTTTCTG
    CTGGTCTGCA TCCTGCACCT CCACTATTTC CATGACCGGT TCCTGGAACT CACAGACCTC
    AAGTCCATCC CCAGCAAAGA AGACAACACC ATCTACAGCC ATGCCAAGGT CAATGGTCGC
    GTGTACCTAA TAATAAATAG GTTAGCCCAC CCTGAAGGAA GCCTCCCAGA CCTAGCCATT
    ATGAACATGA CGGCCAGCCT CGATAAGCCT GAGAGGCAGA AGTTGGCAGA GCCTGGGGAG
    GAGAGGACAG AAGAATGTGC CAAGAAGGCA GAGAAGGGTG AGGCTGGGAA AGACAGTGAT
    GAGTCTGAGG AGGAGGAAGG TGAGGAAGAG GAATCCGAGG AGGAAGAATC CTCAGATCTC
    AGGAACAAGT GGCACCTCGT GATTGACCGG CTCACTGTGC TCTTTCTAAA GTTCCTGGAG
    TATTTCCACA AGTTGCAGGT GTTTATGTGG TGGATCTTGG AGCTGCACAT CATCAAAATC
    GTCTCATCGT ACATTATCTG GGTGACTGTC AAAGAGGTGT CTCTGTTCAA CTATGTATTT
    TTGATTTCTT GGGCATTTGC TCTGCCATAT GCCAAACTCC GCCGTGTGGC TTCCAGTGTC
    TGCACTGTCT GGACGTGTGT GATCATCGTC TGCAAAATGT TGTACCAGCT GCAAACCATT
    AAGCCTGAGA ACTTTTCTGT CAACTGCTCC TTGCCCAATG AAAATCAAAC CAACATACCC
    CTTAATGAGT TGAACAAGTC TCTTCTCTAC AGTGCCCCTG TTGATCCCAC TGAGTGGGTT
    GGCCTGAGGA AGTCCTCCCC TTTACTCGTC TACCTGAGGA ACAACCTCCT GATGTTGGCG
    ATTTTGGCCT TCGAAGTCAC TGTTTACCGA CATCAGGAGT ACTATCGAGG CCGAAACAAC
    TTGACTGCTC CGGTGTCTAA AACTATCTTT CATGACATCA CAAGGTTACA TCTAGATGAT
    GGGCTTATCA ATTGTGCCAA ATATTTTGTA AACTACTTCT TCTACAAGTT TGGCCTAGAG
    ACCTGTTTCC TAATGTCAGT GAACGTAATT GGACAGCGAA TGGACTTCTA CGCCATGATT
    CATGCCTGTT GGCTGATCGG TGTCTTGTAC CGTCGCAGAA GAAAAGCCAT TGCTGAGGTC
    TGGCCCAAGT ACTGCTGCTT CCTGGCTTGC ATCATCACCT TCCAGTACTT TGTCTGCATA
    GGCATCCCTC CTGCCCCCTG CCGAGATTAC CCATGGAGGT TCAAGGGTGC CAACTTCAAT
    GACAACATCA TCAAGTGGCT GTACTTCCCA GATTTCATCG TGCGGCCCAA CCCTGTGTTT
    CTTGTCTATG ACTTCATGCT GCTCCTGTGT GCTTCCTTAC AACGACAGAT TTTTGAGGAC
    GAAAACAAGG CAGCTGTGCG CATCATGGCC GGGGACAATG TCGAGATTTG CATGAACCTT
    GACGCGGCCT CATTCAGCCA GCATAACCCT GTACCAGATT TCATTCACTG CAGATCTTAC
    TTGGACATGT CCAAAGTGAT CATCTTCAGC TACCTCTTCT GGTTCGTCCT CACCATCATC
    TTCATTACCG GAACGACCAG GATCAGCATC TTTTGCATGG GTTACCTGGT GGCCTGTTTC
    TATTTCCTGC TCTTTGGAGG TGATCTGCTC CTGAAACCCA TCAAGAGCAT CCTACGCTAC
    TGGGACTGGC TGATTGCATA CAACGTGTTT GTGATCACCA TGAAAAACAT ACTGTCAATA
    GGAGCCTGTG GATACATCGG TGCACTGGTG AGAAACAGCT GCTGGCTGAT CCAAGCGTTC
    AGCCTGGCCT GCACGGTGAA AGGCTATAAA ATGCCTGAAG ATGACTCCAG ATGCAAGCTG
    CCCAGTGGGG AGGCAGGGAT CATCTGGGAC AGCATCTGCT TTGCGTTTCT CCTGCTACAG
    AGGAGAGTCT TCATGAGTTA CTATTTCCTG CATGTCGTGG CTGACATTAA AGCATCCCAG
    ATCCTGGCAT CCAGAGGGGC CGAACTTTTC CAGGCTACAA TTGTGAAAGC TGTAAAGGCA
    AGAATTGAAG AAGAGAAAAA ATCCATGGAC CAGCTGAAGA GACAGATGGA TCGCATCAAA
    GCCCGGCAGC AAAAGTACAA AAAGGGTAAG GAGAGGATGC TGAGCTTGAC CCAGGAGTCA
    GGGGAAGGCC AGGACATCCA GAAAGTCTCA GAAGAAGACG ATGAAAGAGA AGCAGACAAA
    CAGAAAGCCA AGGGCAAAAA AAAGCAGTGG TGGCGGCCTT GGGTTGATCA TGCTTCCATG
    GTCAGGAGTG GAGATTATTA TTTGTTTGAA ACGGATAGTG AAGAGGAGGA AGAAGAGGAA
    CTCAAAAAGG AGGATGAAGA ACCACCACGG AAGTCTGCAT TCCAGAGAGC TATTGGGAAG
    TTTGCCTCAG CCATTTTAGC CTTGCCAAAG TCTGTCATTA AACTTCCCAA AACTATTCTT
    CAGTATTTAA TCAGAGCTGC AAAGTTCGTC TACCAAGCCT GGATTACCGA TCCTAAAACA
    GCACTCCGAC AGAGGAGGAA AGAGAAGAAG AAAATGGCCA GAGAAGAGCA GAAGGGAAGA
    CGCAAAGGAT CTGGGGAGGG TCCAGTGGAA TGGGAAGACA GAGAAGATGA GCCAATCAAA
    AAGAAATCTG ATGGACCAGA TAATATCATC AAGAGGATAT TTAATATCCT GAAGTTCACC
    TGGGTCCTGT TCCTGGCCAC AGTGGACAGT TTCACTACTT GGCTCAACTC CATCTCAAGA
    GAGCATATCG ACATATCCAC AGTTCTGCGG ATTGAAAGAT GCATGCTGAC CCGAGAGATA
    AAGAAGGGCA ACGTTCCGAC ACGGGAAAGC ATCCACATGT ACTACCAGAA CCACATGATG
    AACCTTTCCA GAGAATCCGG ACTGGACACA ATCGACGAGC ACTCTGGCGC TGGTTCCAGG
    GCCCAGGCAG CACACAGGAT GGATAGCTTA GATTCCCATG ACAGTATCTC CAGCTGTTAC
    ACTGAAGCAA CCCTGCTGAT CTCAAGGCAG TCCACCCTTG ATGATTTAGA TGGACAAGAC
    CCTGTTCCTA AAACAAGCGA GCGTGCTCGG CCTAGGCTCC GTAAAGTGTT CAGCATGGAC
    ATGTCCTCCT CATCAGCTGA CAGTGGCAGT GTAGCATCAA GCGAGCCCAC ACAGTGCACC
    ATGCTGTATT CACGCCAAGG AACTACGGAA ACCATTGAGG AGGTAGAGGC TGAAGCTGAG
    GAGGAGGTGG TAGAGAGTCT GGAGCCTGAG CTCCATGATG CCGAAGAGAA GGAATATGCA
    GCTGACTATG AAGCAGGGGT AGAGGAGGTC AGCCTCACTC CAGATGAGGA GTTGCCTCAG
    TTCTCAACAG ATGACGGTGA CGTGGAGGCC CCGCCCTCCT ACAGCAAGGC TGTCAGCTTT
    GAGCACCTCT CGTTTGCATC ACAAGATGAC TCCGGGGGCA AGAACCACAT GGTGGTCAGC
    CCTGACGACA GCCGCACAGA CAAGCTGGAG TCCAGTATCT TACCGCCCCT GACTCACGAG
    CTGACAGCCA GCGATCTGCT CATGAGCAAG ATGTTCCGTG ATGATGAGCT TGAAGAATCT
    GAGAAATTCT ACGTGGACCA GCCCCGGTTC TTGCTGCTAT TCTATGCCAT GTATAACACT
    CTGGTAGCCC GCTCGGAAAT GGTGTGCTAT TTCGTGATCA TCCTGAACCA CATGATCTCT
    GCCTCCATCA TTACTCTTCT GTTGCCCATC CTGATCTTCC TCTGGGCCAT GCTGTCCGTC
    CCCAGGCCCA GCCGGCGCTT CTGGATGATG GCCATCGTCT ACACTGAGGT GGCGATTGTA
    GTGAAATATT TCTTCCAGTT TGGCTTCTTT CCTTGGAATA AGGACTTGGA AATTTACAAG
    GACAGGCCCT ACTTTCCACC AAACATCATA GGGGTGGAAA AGAAAGAGGG CTATGTTCTT
    TACGACCTCA TTCAGCTGCT GGCTCTGTTC TTCCATCGAT CCATTCTGAA GTGTCATGGC
    CTATGGGACG AGGATGACAT TGTTGACAGT AACACAGACA AGGAAGGATC TGATGATGAG
    CTGTCCCTCG GTCAGGGCAG AAGAGGCTCC TCAGAGTCAC TGAAGTCCAT CAACTTGGCT
    GCCTCTGTAG AGTCCGTCCA TGTGACCTTC CCAGAGCAGC CAGCAGCCAT CCGGAGAAAA
    CGCTCCTGCA GCAGCTCGCA GATCTCCCCA CGGTCCAGCT TCTCTTCAAA TAGGTCCAAA
    AGAGGCAGCA CAAGCACCCG AAACAGCAGC CAAAAAGGAA GCAGTGTTTT GAGCATTAAA
    CAAAAAAGCA AAAGAGAACT TTACATGGAA AAGCTTCAAG AACATTTAAT CAAAGCAAAG
    GCCTTTACCA TAAAGAAAAC TCTACAAATC TATGTACCCA TTCGGCAATT CTTCTATGAC
    CTCATCCACC CAGACTACAG TGCTGTGACT GACGTGTATG TGCTGATGTT CCTGGCCGAC
    ACCGTGGACT TCATCATCAT CGTCTTTGGC TTCTGGGCTT TTGGGAAACA CTCGGCAGCT
    GCAGATATCA CCTCTTCCCT ATCAGAAGAC CAGGTCCCCG GGCCATTCCT GGTGATGGTT
    CTCATTCAGT TTGGAACCAT GGTGGTGGAC CGTGCACTCT ACCTCAGGAA GACTGTGCTT
    GGCAAGGTCA TTTTCCAGGT CATCCTCGTG TTTGGAATTC ACTTCTGGAT GTTCTTCATC
    CTACCCAGTG TGACTGAGAG GAAATTCAGC CAGAACCTGG TGGCACAGCT CTGGTACTTT
    GTGAAATGTG TGTACTTTGG GTTGTCTGCC TACCAAATCC GCTGTGGCTA CCCAACACGT
    GTGCTCGGAA ACTTCCTCAC GAAGAGCTAC AATTATGTCA ACCTCTTCTT GTTTCAAGGG
    TTTCGTCTCG TCCCATTTCT GACAGAGCTG CGGGCTGTGA TGGACTGGGT GTGGACAGAT
    ACAACCTTGA GCCTCTCCAG CTGGATTTGT GTGGAGGACA TTTACGCACA CATTTTCATC
    TTAAAGTGCT GGCGAGAGTC GGAAAAGAGA TACCCTCAAC CCAGGGGCCA GAAGAAGAAG
    AAAGCTGTGA AGTATGGCAT GGGCGGAATG ATCATTGTCC TGCTCATCTG TATCGTCTGG
    TTTCCTCTGC TCTTCATGTC TTTGATCAAG TCTGTCGCAG GGGTTATTAA CCAACCCCTG
    GATGTGTCCG TCACAATAAC CCTGGGAGGC TATCAGCCTA TCTTCACAAT GAGTGCCCAG
    CAAAGCCAGC TGAAAGTTAT GGATGGTACA AAGTATAACG AATTCATAAA GTCTTTCGGC
    CCCAACAGTG GCGCTATGCA ATTTCTGGAG AATTATGAAA GGCAAGACAT AACAGTAGCG
    GAACTGGAAG GGAATTCGAA TTCTTTGTGG ACCATCAGCC CTCCCAGTAA GCAGAAAATG
    ATACAGGAGC TCACCGACCC CAATAGTTGC TTCTCTGTTG TTTTTTCATG GAGTATCCAG
    AGAAACATGA CTCTGGGTGC AAAGGCTGAA ATAGCAACGG ACAAGCTTTC TTTTCCTCTC
    ACGGATGCGA CACGAAGTAG CATAGCCAAA ATGATTGCTG GCAATGACAC AGAAAGTTCC
    AACACACCAG TGACAATAGA AAAGATCTAC CCATACTATG TGAAGGCACC CAGTGACTCA
    AACTCAAAGC CTATAAAACA GCTTCTGTCA GAAAACAATT TCATGAATAT CACCATCATT
    TTGTTCAGAG ACAATGTCAC AAAGTCCAAT AGTGAGTGGT GGGTTCTCAA CCTGACTGGA
    AGTAGGATAT TCAATCAGGG ATCCCAAGCC TTGGAGCTGG TGGTCTTCAA TGACAAAGTC
    AGCCCCCCAA GTCTGGGCTT CTTGGCTGGC TATGGCATCA TGGGATTGTA TGCATCCGTT
    GTCCTTGTAA TTGGGAAGTT TGTTCGCGAA TTCTTCAGTG GGATTTCTCA CTCTATCATG
    TTTGAAGAGC TTCCAAATGT GGACAGAATT TTGAAGTTAT GCACAGATAT ATTTCTCGTA
    CGGGAGACAG GGGAACTGGA GCTGGAGGAA GACCTCTATG CCAAATTGAT ATTCCTGTAC
    AGGTCGCCAG AAACCATGAT CAAGTGGACT AGAGAAAAAA CAAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa54718
    Clone ID Related Accession (Same CDS sequence) XM_017601116.1 , XM_017587877.1
    Accession Version XM_017601116.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8562bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product piezo-type mechanosensitive ion channel component 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005117.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTCCCCAT CTGCCTGGCA 
    GTAGCATGTG CATTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCG ACGATGCAAG GACACACAGG GCGGCTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCCTTT CTGCTCCTTC ACATCATTTT CCACATCACA
    CTGGCCAGCC TGGAAGCTCA GCACCGCATC GCACCCGCTT ACAACTGCTC AACTTGGGAA
    AAGACCTTCC GGCAGATCGG CTTCGAAAGC TTGAAGGGAG CTGATGCTGG CAATGGCATC
    AGGGTGTTTG TGCCTGATAT CGGGATGTTC ATTGCGAGCC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACGGAA GAAGTAGCCC AATTTAATTC CGAGTGCGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GACTCAGAAG AAGCGCTGAT TTATGAGGAG
    GACTTAGATG GGGAAGAAGG CATGGAAGGC GAGCTGGAAG AAAGCACAAA GTTAAAAATG
    TTTCGCAGGC TCGCCTCCGT CGCTTCCAAG CTTAAGGAGT TCATCGGCAA TATGATAACC
    ACGGCGGGCA AGGTGGTGGT GACCATCCTG TTGGGCTCCT CTGGCATGAT GCTGCCATCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT CTGGGTCTGT GCACCTGGTG GTCCTGGTGC
    CGAACTTTTG ACCCATTGCT GTTTGGCTGC CTCTGTGTCC TGTTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTTCAGTTCT TCCAAGAAGC AGTCCCACCC
    AATGACTACT ATGCAAGGTT GTTTGGAATC AAATCCGTTA TTCAAACAGA CTGCTCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTACAC GCTGGCCACG CTGATCCGCA TTTGGTTGCA AGAGCCTCTT
    GTGCAGGAGG AGATGTCGAA GGAAGAGGAG GGTGCACTGG ATGGCTGCTC CAATCAGAAC
    ACAGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    GGCCTGCTGG TGACTGTGAA CGGCAACCCT GTGGACTACC ACACCATCCA CCCAAGTCTG
    CCCATAGAGA ATGGCCCCGC CAAGACGGAC TTGTATACTA CTCCTCAGTA CCGGTGGGAG
    CCCTCAGAGG AGTCCTCAGA AAAGAAAGAG GAGGAAGAGG ACAAGAGAGA AGACTCAGAA
    GGGGAAGGGA GCCAAGAGGA AAAACGGAGT GTGAGAATTC ATGCCATGGT CGCCGTGTTC
    CAGTTCATCA TGAAGCAGAG TTACATCTGT GCCCTCATCG CCATGATGGC ATGGAGCATC
    ACCTACCACA GCTGGCTGAC ATTTGTGCTG TTGATCTGGT CGTGCACTCT CTGGATGATT
    CGCAACAGGA GGAAGTACGC CATGATCAGC TCTCCGTTCA TGGTTGTCTA TGCCAATCTG
    CTGCTGGTAC TGCAGTACAT ATGGAGCTTT GAACTTCCAG AAATCAAGAA AGTCCCTGGA
    TTTCTAGAAA AGAAAGAGCC TGGAGAGCTT GCCTCAAAGA TCCTCTTCAC CATCACATTT
    TGGCTGCTCC TGAGGCAGCA TCTCACAGAG CAGAAGGCCC TTCAGGAAAA GGAAGCACTT
    CTGTCTGAAG TCAAAATTGG AAGTCAAGAA CTTGACGAAA AGGATGAGGA ACTCCAAGAT
    ATACAAGTGG AAGGAGAGCC CATAGAGAAG GAAGAAGAGG AGGAGGAAAT AAAGGAAGAC
    AGACATGAGG TAAAGAAGGA AGAGGAAGAG GAAGAGGAAG ATGACGATGA CCAGGACATC
    ATGAAGGTTC TGGGCAATCT GGTGGTGGCC CTCTTCATCA AGTATTGGAT CTACGTCTGC
    GGGGGCATGT TCTTTTTTGT CAGCTTTGAG GGGAAAATCG TCATGTACAA AATCATCTAC
    ATGGTGCTGT TCCTGTTCTG TGTGGCCTTG TACCAGGTGC ACTACGAGTG GTGGAGGAAG
    ATCCTGAAAT ACTTCTGGAT GTCGGTGGTC ATTTACACCA TGCTGGTGCT CATCTTCATC
    TACACTTACC AGTTTGAGAA CTTCCCAGGC CTGTGGCAAA ACATGACCGG GTTAAAGAAA
    GAGAAGCTTG AGGATCTGGG CCTGAAGCAG TTCACTGTGG CCGAACTGTT CACACGAATA
    TTCATCCCCA CCTCCTTTCT GCTGGTCTGC ATCCTGCACC TCCACTATTT CCATGACCGG
    TTCCTGGAAC TCACAGACCT CAAGTCCATC CCCAGCAAAG AAGACAACAC CATCTACAGC
    CATGCCAAGG TCAATGGTCG CGTGTACCTA ATAATAAATA GCATCAAGAA AAAATTACCA
    ATCCACCAGA ATGAGTTAGC CCACCCTGAA GGAAGCCTCC CAGACCTAGC CATTATGAAC
    ATGACGGCCA GCCTCGATAA GCCTGAGAGG CAGAAGTTGG CAGAGCCTGG GGAGGAGAGG
    ACAGAAGAAT GTGCCAAGAA GGCAGAGAAG GGTGAGGCTG GGAAAGACAG TGATGAGTCT
    GAGGAGGAGG AAGGTGAGGA AGAGGAATCC GAGGAGGAAG AATCCTCAGA TCTCAGGAAC
    AAGTGGCACC TCGTGATTGA CCGGCTCACT GTGCTCTTTC TAAAGTTCCT GGAGTATTTC
    CACAAGTTGC AGGTGTTTAT GTGGTGGATC TTGGAGCTGC ACATCATCAA AATCGTCTCA
    TCGTACATTA TCTGGGTGAC TGTCAAAGAG GTGTCTCTGT TCAACTATGT ATTTTTGATT
    TCTTGGGCAT TTGCTCTGCC ATATGCCAAA CTCCGCCGTG TGGCTTCCAG TGTCTGCACT
    GTCTGGACGT GTGTGATCAT CGTCTGCAAA ATGTTGTACC AGCTGCAAAC CATTAAGCCT
    GAGAACTTTT CTGTCAACTG CTCCTTGCCC AATGAAAATC AAACCAACAT ACCCCTTAAT
    GAGTTGAACA AGTCTCTTCT CTACAGTGCC CCTGTTGATC CCACTGAGTG GGTTGGCCTG
    AGGAAGTCCT CCCCTTTACT CGTCTACCTG AGGAACAACC TCCTGATGTT GGCGATTTTG
    GCCTTCGAAG TCACTGTTTA CCGACATCAG GAGTACTATC GAGGCCGAAA CAACTTGACT
    GCTCCGGTGT CTAAAACTAT CTTTCATGAC ATCACAAGGT TACATCTAGA TGATGGGCTT
    ATCAATTGTG CCAAATATTT TGTAAACTAC TTCTTCTACA AGTTTGGCCT AGAGACCTGT
    TTCCTAATGT CAGTGAACGT AATTGGACAG CGAATGGACT TCTACGCCAT GATTCATGCC
    TGTTGGCTGA TCGGTGTCTT GTACCGTCGC AGAAGAAAAG CCATTGCTGA GGTCTGGCCC
    AAGTACTGCT GCTTCCTGGC TTGCATCATC ACCTTCCAGT ACTTTGTCTG CATAGGCATC
    CCTCCTGCCC CCTGCCGAGA TTACCCATGG AGGTTCAAGG GTGCCAACTT CAATGACAAC
    ATCATCAAGT GGCTGTACTT CCCAGATTTC ATCGTGCGGC CCAACCCTGT GTTTCTTGTC
    TATGACTTCA TGCTGCTCCT GTGTGCTTCC TTACAACGAC AGATTTTTGA GGACGAAAAC
    AAGGCAGCTG TGCGCATCAT GGCCGGGGAC AATGTCGAGA TTTGCATGAA CCTTGACGCG
    GCCTCATTCA GCCAGCATAA CCCTGTACCA GATTTCATTC ACTGCAGATC TTACTTGGAC
    ATGTCCAAAG TGATCATCTT CAGCTACCTC TTCTGGTTCG TCCTCACCAT CATCTTCATT
    ACCGGAACGA CCAGGATCAG CATCTTTTGC ATGGGTTACC TGGTGGCCTG TTTCTATTTC
    CTGCTCTTTG GAGGTGATCT GCTCCTGAAA CCCATCAAGA GCATCCTACG CTACTGGGAC
    TGGCTGATTG CATACAACGT GTTTGTGATC ACCATGAAAA ACATACTGTC AATAGGAGCC
    TGTGGATACA TCGGTGCACT GGTGAGAAAC AGCTGCTGGC TGATCCAAGC GTTCAGCCTG
    GCCTGCACGG TGAAAGGCTA TAAAATGCCT GAAGATGACT CCAGATGCAA GCTGCCCAGT
    GGGGAGGCAG GGATCATCTG GGACAGCATC TGCTTTGCGT TTCTCCTGCT ACAGAGGAGA
    GTCTTCATGA GTTACTATTT CCTGCATGTC GTGGCTGACA TTAAAGCATC CCAGATCCTG
    GCATCCAGAG GGGCCGAACT TTTCCAGGCT ACAATTGTGA AAGCTGTAAA GGCAAGAATT
    GAAGAAGAGA AAAAATCCAT GGACCAGCTG AAGAGACAGA TGGATCGCAT CAAAGCCCGG
    CAGCAAAAGT ACAAAAAGGG TAAGGAGAGG ATGCTGAGCT TGACCCAGGA GTCAGGGGAA
    GGCCAGGACA TCCAGAAAGT CTCAGAAGAA GACGATGAAA GAGAAGCAGA CAAACAGAAA
    GCCAAGGGCA AAAAAAAGCA GTGGTGGCGG CCTTGGGTTG ATCATGCTTC CATGGTCAGG
    AGTGGAGATT ATTATTTGTT TGAAACGGAT AGTGAAGAGG AGGAAGAAGA GGAACTCAAA
    AAGGAGGATG AAGAACCACC ACGGAAGTCT GCATTCCAGA GAGCTATTGG GAAGTTTGCC
    TCAGCCATTT TAGCCTTGCC AAAGTCTGTC ATTAAACTTC CCAAAACTAT TCTTCAGTAT
    TTAATCAGAG CTGCAAAGTT CGTCTACCAA GCCTGGATTA CCGATCCTAA AACAGCACTC
    CGACAGAGGA GGAAAGAGAA GAAGAAAATG GCCAGAGAAG AGCAGAAGGG AAGACGCAAA
    GGATCTGGGG AGGGTCCAGT GGAATGGGAA GACAGAGAAG ATGAGCCAAT CAAAAAGAAA
    TCTGATGGAC CAGATAATAT CATCAAGAGG ATATTTAATA TCCTGAAGTT CACCTGGGTC
    CTGTTCCTGG CCACAGTGGA CAGTTTCACT ACTTGGCTCA ACTCCATCTC AAGAGAGCAT
    ATCGACATAT CCACAGTTCT GCGGATTGAA AGATGCATGC TGACCCGAGA GATAAAGAAG
    GGCAACGTTC CGACACGGGA AAGCATCCAC ATGTACTACC AGAACCACAT GATGAACCTT
    TCCAGAGAAT CCGGACTGGA CACAATCGAC GAGCACTCTG GCGCTGGTTC CAGGGCCCAG
    GCAGCACACA GGATGGATAG CTTAGATTCC CATGACAGTA TCTCCAGCTG TTACACTGAA
    GCAACCCTGC TGATCTCAAG GCAGTCCACC CTTGATGATT TAGATGGACA AGACCCTGTT
    CCTAAAACAA GCGAGCGTGC TCGGCCTAGG CTCCGTAAAG TGTTCAGCAT GGACATGTCC
    TCCTCATCAG CTGACAGTGG CAGTGTAGCA TCAAGCGAGC CCACACAGTG CACCATGCTG
    TATTCACGCC AAGGAACTAC GGAAACCATT GAGGAGGTAG AGGCTGAAGC TGAGGAGGAG
    GTGGTAGAGA GTCTGGAGCC TGAGCTCCAT GATGCCGAAG AGAAGGAATA TGCAGCTGAC
    TATGAAGCAG GGGTAGAGGA GGTCAGCCTC ACTCCAGATG AGGAGTTGCC TCAGTTCTCA
    ACAGATGACG GTGACGTGGA GGCCCCGCCC TCCTACAGCA AGGCTGTCAG CTTTGAGCAC
    CTCTCGTTTG CATCACAAGA TGACTCCGGG GGCAAGAACC ACATGGTGGT CAGCCCTGAC
    GACAGCCGCA CAGACAAGCT GGAGTCCAGT ATCTTACCGC CCCTGACTCA CGAGCTGACA
    GCCAGCGATC TGCTCATGAG CAAGATGTTC CGTGATGATG AGCTTGAAGA ATCTGAGAAA
    TTCTACGTGG ACCAGCCCCG GTTCTTGCTG CTATTCTATG CCATGTATAA CACTCTGGTA
    GCCCGCTCGG AAATGGTGTG CTATTTCGTG ATCATCCTGA ACCACATGAT CTCTGCCTCC
    ATCATTACTC TTCTGTTGCC CATCCTGATC TTCCTCTGGG CCATGCTGTC CGTCCCCAGG
    CCCAGCCGGC GCTTCTGGAT GATGGCCATC GTCTACACTG AGGTGGCGAT TGTAGTGAAA
    TATTTCTTCC AGTTTGGCTT CTTTCCTTGG AATAAGGACT TGGAAATTTA CAAGGACAGG
    CCCTACTTTC CACCAAACAT CATAGGGGTG GAAAAGAAAG AGGGCTATGT TCTTTACGAC
    CTCATTCAGC TGCTGGCTCT GTTCTTCCAT CGATCCATTC TGAAGTGTCA TGGCCTATGG
    GACGAGGATG ACATTGTTGA CAGTAACACA GACAAGGAAG GATCTGATGA TGAGCTGTCC
    CTCGGTCAGG GCAGAAGAGG CTCCTCAGAG TCACTGAAGT CCATCAACTT GGCTGCCTCT
    GTAGAGTCCG TCCATGTGAC CTTCCCAGAG CAGCCAGCAG CCATCCGGAG AAAACGCTCC
    TGCAGCAGCT CGCAGATCTC CCCACGGTCC AGCTTCTCTT CAAATAGGTC CAAAAGAGGC
    AGCACAAGCA CCCGAAACAG CAGCCAAAAA GGAAGCAGTG TTTTGAGCAT TAAACAAAAA
    AGCAAAAGAG AACTTTACAT GGAAAAGCTT CAAGAACATT TAATCAAAGC AAAGGCCTTT
    ACCATAAAGA AAACTCTACA AATCTATGTA CCCATTCGGC AATTCTTCTA TGACCTCATC
    CACCCAGACT ACAGTGCTGT GACTGACGTG TATGTGCTGA TGTTCCTGGC CGACACCGTG
    GACTTCATCA TCATCGTCTT TGGCTTCTGG GCTTTTGGGA AACACTCGGC AGCTGCAGAT
    ATCACCTCTT CCCTATCAGA AGACCAGGTC CCCGGGCCAT TCCTGGTGAT GGTTCTCATT
    CAGTTTGGAA CCATGGTGGT GGACCGTGCA CTCTACCTCA GGAAGACTGT GCTTGGCAAG
    GTCATTTTCC AGGTCATCCT CGTGTTTGGA ATTCACTTCT GGATGTTCTT CATCCTACCC
    AGTGTGACTG AGAGGAAATT CAGCCAGAAC CTGGTGGCAC AGCTCTGGTA CTTTGTGAAA
    TGTGTGTACT TTGGGTTGTC TGCCTACCAA ATCCGCTGTG GCTACCCAAC ACGTGTGCTC
    GGAAACTTCC TCACGAAGAG CTACAATTAT GTCAACCTCT TCTTGTTTCA AGGGTTTCGT
    CTCGTCCCAT TTCTGACAGA GCTGCGGGCT GTGATGGACT GGGTGTGGAC AGATACAACC
    TTGAGCCTCT CCAGCTGGAT TTGTGTGGAG GACATTTACG CACACATTTT CATCTTAAAG
    TGCTGGCGAG AGTCGGAAAA GAGATACCCT CAACCCAGGG GCCAGAAGAA GAAGAAAGCT
    GTGAAGTATG GCATGGGCGG AATGATCATT GTCCTGCTCA TCTGTATCGT CTGGTTTCCT
    CTGCTCTTCA TGTCTTTGAT CAAGTCTGTC GCAGGGGTTA TTAACCAACC CCTGGATGTG
    TCCGTCACAA TAACCCTGGG AGGCTATCAG CCTATCTTCA CAATGAGTGC CCAGCAAAGC
    CAGCTGAAAG TTATGGATGG TACAAAGTAT AACGAATTCA TAAAGTCTTT CGGCCCCAAC
    AGTGGCGCTA TGCAATTTCT GGAGAATTAT GAAAGGCAAG ACATAACAGT AGCGGAACTG
    GAAGGGAATT CGAATTCTTT GTGGACCATC AGCCCTCCCA GTAAGCAGAA AATGATACAG
    GAGCTCACCG ACCCCAATAG TTGCTTCTCT GTTGTTTTTT CATGGAGTAT CCAGAGAAAC
    ATGACTCTGG GTGCAAAGGC TGAAATAGCA ACGGACAAGC TTTCTTTTCC TCTCACGGAT
    GCGACACGAA GTAGCATAGC CAAAATGATT GCTGGCAATG ACACAGAAAG TTCCAACACA
    CCAGTGACAA TAGAAAAGAT CTACCCATAC TATGTGAAGG CACCCAGTGA CTCAAACTCA
    AAGCCTATAA AACAGCTTCT GTCAGAAAAC AATTTCATGA ATATCACCAT CATTTTGTTC
    AGAGACAATG TCACAAAGTC CAATAGTGAG TGGTGGGTTC TCAACCTGAC TGGAAGTAGG
    ATATTCAATC AGGGATCCCA AGCCTTGGAG CTGGTGGTCT TCAATGACAA AGTCAGCCCC
    CCAAGTCTGG GCTTCTTGGC TGGCTATGGC ATCATGGGAT TGTATGCATC CGTTGTCCTT
    GTAATTGGGA AGTTTGTTCG CGAATTCTTC AGTGGGATTT CTCACTCTAT CATGTTTGAA
    GAGCTTCCAA ATGTGGACAG AATTTTGAAG TTATGCACAG ATATATTTCT CGTACGGGAG
    ACAGGGGAAC TGGAGCTGGA GGAAGACCTC TATGCCAAAT TGATATTCCT GTACAGGTCG
    CCAGAAACCA TGATCAAGTG GACTAGAGAA AAAACAAATT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017456605.1
    CDS940..9501
    Translation

    Target ORF information:

    RefSeq Version XM_017601116.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017601116.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTCCCCAT CTGCCTGGCA 
    GTAGCATGTG CATTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCG ACGATGCAAG GACACACAGG GCGGCTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCCTTT CTGCTCCTTC ACATCATTTT CCACATCACA
    CTGGCCAGCC TGGAAGCTCA GCACCGCATC GCACCCGCTT ACAACTGCTC AACTTGGGAA
    AAGACCTTCC GGCAGATCGG CTTCGAAAGC TTGAAGGGAG CTGATGCTGG CAATGGCATC
    AGGGTGTTTG TGCCTGATAT CGGGATGTTC ATTGCGAGCC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACGGAA GAAGTAGCCC AATTTAATTC CGAGTGCGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GACTCAGAAG AAGCGCTGAT TTATGAGGAG
    GACTTAGATG GGGAAGAAGG CATGGAAGGC GAGCTGGAAG AAAGCACAAA GTTAAAAATG
    TTTCGCAGGC TCGCCTCCGT CGCTTCCAAG CTTAAGGAGT TCATCGGCAA TATGATAACC
    ACGGCGGGCA AGGTGGTGGT GACCATCCTG TTGGGCTCCT CTGGCATGAT GCTGCCATCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT CTGGGTCTGT GCACCTGGTG GTCCTGGTGC
    CGAACTTTTG ACCCATTGCT GTTTGGCTGC CTCTGTGTCC TGTTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTTCAGTTCT TCCAAGAAGC AGTCCCACCC
    AATGACTACT ATGCAAGGTT GTTTGGAATC AAATCCGTTA TTCAAACAGA CTGCTCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTACAC GCTGGCCACG CTGATCCGCA TTTGGTTGCA AGAGCCTCTT
    GTGCAGGAGG AGATGTCGAA GGAAGAGGAG GGTGCACTGG ATGGCTGCTC CAATCAGAAC
    ACAGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    GGCCTGCTGG TGACTGTGAA CGGCAACCCT GTGGACTACC ACACCATCCA CCCAAGTCTG
    CCCATAGAGA ATGGCCCCGC CAAGACGGAC TTGTATACTA CTCCTCAGTA CCGGTGGGAG
    CCCTCAGAGG AGTCCTCAGA AAAGAAAGAG GAGGAAGAGG ACAAGAGAGA AGACTCAGAA
    GGGGAAGGGA GCCAAGAGGA AAAACGGAGT GTGAGAATTC ATGCCATGGT CGCCGTGTTC
    CAGTTCATCA TGAAGCAGAG TTACATCTGT GCCCTCATCG CCATGATGGC ATGGAGCATC
    ACCTACCACA GCTGGCTGAC ATTTGTGCTG TTGATCTGGT CGTGCACTCT CTGGATGATT
    CGCAACAGGA GGAAGTACGC CATGATCAGC TCTCCGTTCA TGGTTGTCTA TGCCAATCTG
    CTGCTGGTAC TGCAGTACAT ATGGAGCTTT GAACTTCCAG AAATCAAGAA AGTCCCTGGA
    TTTCTAGAAA AGAAAGAGCC TGGAGAGCTT GCCTCAAAGA TCCTCTTCAC CATCACATTT
    TGGCTGCTCC TGAGGCAGCA TCTCACAGAG CAGAAGGCCC TTCAGGAAAA GGAAGCACTT
    CTGTCTGAAG TCAAAATTGG AAGTCAAGAA CTTGACGAAA AGGATGAGGA ACTCCAAGAT
    ATACAAGTGG AAGGAGAGCC CATAGAGAAG GAAGAAGAGG AGGAGGAAAT AAAGGAAGAC
    AGACATGAGG TAAAGAAGGA AGAGGAAGAG GAAGAGGAAG ATGACGATGA CCAGGACATC
    ATGAAGGTTC TGGGCAATCT GGTGGTGGCC CTCTTCATCA AGTATTGGAT CTACGTCTGC
    GGGGGCATGT TCTTTTTTGT CAGCTTTGAG GGGAAAATCG TCATGTACAA AATCATCTAC
    ATGGTGCTGT TCCTGTTCTG TGTGGCCTTG TACCAGGTGC ACTACGAGTG GTGGAGGAAG
    ATCCTGAAAT ACTTCTGGAT GTCGGTGGTC ATTTACACCA TGCTGGTGCT CATCTTCATC
    TACACTTACC AGTTTGAGAA CTTCCCAGGC CTGTGGCAAA ACATGACCGG GTTAAAGAAA
    GAGAAGCTTG AGGATCTGGG CCTGAAGCAG TTCACTGTGG CCGAACTGTT CACACGAATA
    TTCATCCCCA CCTCCTTTCT GCTGGTCTGC ATCCTGCACC TCCACTATTT CCATGACCGG
    TTCCTGGAAC TCACAGACCT CAAGTCCATC CCCAGCAAAG AAGACAACAC CATCTACAGC
    CATGCCAAGG TCAATGGTCG CGTGTACCTA ATAATAAATA GCATCAAGAA AAAATTACCA
    ATCCACCAGA ATGAGTTAGC CCACCCTGAA GGAAGCCTCC CAGACCTAGC CATTATGAAC
    ATGACGGCCA GCCTCGATAA GCCTGAGAGG CAGAAGTTGG CAGAGCCTGG GGAGGAGAGG
    ACAGAAGAAT GTGCCAAGAA GGCAGAGAAG GGTGAGGCTG GGAAAGACAG TGATGAGTCT
    GAGGAGGAGG AAGGTGAGGA AGAGGAATCC GAGGAGGAAG AATCCTCAGA TCTCAGGAAC
    AAGTGGCACC TCGTGATTGA CCGGCTCACT GTGCTCTTTC TAAAGTTCCT GGAGTATTTC
    CACAAGTTGC AGGTGTTTAT GTGGTGGATC TTGGAGCTGC ACATCATCAA AATCGTCTCA
    TCGTACATTA TCTGGGTGAC TGTCAAAGAG GTGTCTCTGT TCAACTATGT ATTTTTGATT
    TCTTGGGCAT TTGCTCTGCC ATATGCCAAA CTCCGCCGTG TGGCTTCCAG TGTCTGCACT
    GTCTGGACGT GTGTGATCAT CGTCTGCAAA ATGTTGTACC AGCTGCAAAC CATTAAGCCT
    GAGAACTTTT CTGTCAACTG CTCCTTGCCC AATGAAAATC AAACCAACAT ACCCCTTAAT
    GAGTTGAACA AGTCTCTTCT CTACAGTGCC CCTGTTGATC CCACTGAGTG GGTTGGCCTG
    AGGAAGTCCT CCCCTTTACT CGTCTACCTG AGGAACAACC TCCTGATGTT GGCGATTTTG
    GCCTTCGAAG TCACTGTTTA CCGACATCAG GAGTACTATC GAGGCCGAAA CAACTTGACT
    GCTCCGGTGT CTAAAACTAT CTTTCATGAC ATCACAAGGT TACATCTAGA TGATGGGCTT
    ATCAATTGTG CCAAATATTT TGTAAACTAC TTCTTCTACA AGTTTGGCCT AGAGACCTGT
    TTCCTAATGT CAGTGAACGT AATTGGACAG CGAATGGACT TCTACGCCAT GATTCATGCC
    TGTTGGCTGA TCGGTGTCTT GTACCGTCGC AGAAGAAAAG CCATTGCTGA GGTCTGGCCC
    AAGTACTGCT GCTTCCTGGC TTGCATCATC ACCTTCCAGT ACTTTGTCTG CATAGGCATC
    CCTCCTGCCC CCTGCCGAGA TTACCCATGG AGGTTCAAGG GTGCCAACTT CAATGACAAC
    ATCATCAAGT GGCTGTACTT CCCAGATTTC ATCGTGCGGC CCAACCCTGT GTTTCTTGTC
    TATGACTTCA TGCTGCTCCT GTGTGCTTCC TTACAACGAC AGATTTTTGA GGACGAAAAC
    AAGGCAGCTG TGCGCATCAT GGCCGGGGAC AATGTCGAGA TTTGCATGAA CCTTGACGCG
    GCCTCATTCA GCCAGCATAA CCCTGTACCA GATTTCATTC ACTGCAGATC TTACTTGGAC
    ATGTCCAAAG TGATCATCTT CAGCTACCTC TTCTGGTTCG TCCTCACCAT CATCTTCATT
    ACCGGAACGA CCAGGATCAG CATCTTTTGC ATGGGTTACC TGGTGGCCTG TTTCTATTTC
    CTGCTCTTTG GAGGTGATCT GCTCCTGAAA CCCATCAAGA GCATCCTACG CTACTGGGAC
    TGGCTGATTG CATACAACGT GTTTGTGATC ACCATGAAAA ACATACTGTC AATAGGAGCC
    TGTGGATACA TCGGTGCACT GGTGAGAAAC AGCTGCTGGC TGATCCAAGC GTTCAGCCTG
    GCCTGCACGG TGAAAGGCTA TAAAATGCCT GAAGATGACT CCAGATGCAA GCTGCCCAGT
    GGGGAGGCAG GGATCATCTG GGACAGCATC TGCTTTGCGT TTCTCCTGCT ACAGAGGAGA
    GTCTTCATGA GTTACTATTT CCTGCATGTC GTGGCTGACA TTAAAGCATC CCAGATCCTG
    GCATCCAGAG GGGCCGAACT TTTCCAGGCT ACAATTGTGA AAGCTGTAAA GGCAAGAATT
    GAAGAAGAGA AAAAATCCAT GGACCAGCTG AAGAGACAGA TGGATCGCAT CAAAGCCCGG
    CAGCAAAAGT ACAAAAAGGG TAAGGAGAGG ATGCTGAGCT TGACCCAGGA GTCAGGGGAA
    GGCCAGGACA TCCAGAAAGT CTCAGAAGAA GACGATGAAA GAGAAGCAGA CAAACAGAAA
    GCCAAGGGCA AAAAAAAGCA GTGGTGGCGG CCTTGGGTTG ATCATGCTTC CATGGTCAGG
    AGTGGAGATT ATTATTTGTT TGAAACGGAT AGTGAAGAGG AGGAAGAAGA GGAACTCAAA
    AAGGAGGATG AAGAACCACC ACGGAAGTCT GCATTCCAGA GAGCTATTGG GAAGTTTGCC
    TCAGCCATTT TAGCCTTGCC AAAGTCTGTC ATTAAACTTC CCAAAACTAT TCTTCAGTAT
    TTAATCAGAG CTGCAAAGTT CGTCTACCAA GCCTGGATTA CCGATCCTAA AACAGCACTC
    CGACAGAGGA GGAAAGAGAA GAAGAAAATG GCCAGAGAAG AGCAGAAGGG AAGACGCAAA
    GGATCTGGGG AGGGTCCAGT GGAATGGGAA GACAGAGAAG ATGAGCCAAT CAAAAAGAAA
    TCTGATGGAC CAGATAATAT CATCAAGAGG ATATTTAATA TCCTGAAGTT CACCTGGGTC
    CTGTTCCTGG CCACAGTGGA CAGTTTCACT ACTTGGCTCA ACTCCATCTC AAGAGAGCAT
    ATCGACATAT CCACAGTTCT GCGGATTGAA AGATGCATGC TGACCCGAGA GATAAAGAAG
    GGCAACGTTC CGACACGGGA AAGCATCCAC ATGTACTACC AGAACCACAT GATGAACCTT
    TCCAGAGAAT CCGGACTGGA CACAATCGAC GAGCACTCTG GCGCTGGTTC CAGGGCCCAG
    GCAGCACACA GGATGGATAG CTTAGATTCC CATGACAGTA TCTCCAGCTG TTACACTGAA
    GCAACCCTGC TGATCTCAAG GCAGTCCACC CTTGATGATT TAGATGGACA AGACCCTGTT
    CCTAAAACAA GCGAGCGTGC TCGGCCTAGG CTCCGTAAAG TGTTCAGCAT GGACATGTCC
    TCCTCATCAG CTGACAGTGG CAGTGTAGCA TCAAGCGAGC CCACACAGTG CACCATGCTG
    TATTCACGCC AAGGAACTAC GGAAACCATT GAGGAGGTAG AGGCTGAAGC TGAGGAGGAG
    GTGGTAGAGA GTCTGGAGCC TGAGCTCCAT GATGCCGAAG AGAAGGAATA TGCAGCTGAC
    TATGAAGCAG GGGTAGAGGA GGTCAGCCTC ACTCCAGATG AGGAGTTGCC TCAGTTCTCA
    ACAGATGACG GTGACGTGGA GGCCCCGCCC TCCTACAGCA AGGCTGTCAG CTTTGAGCAC
    CTCTCGTTTG CATCACAAGA TGACTCCGGG GGCAAGAACC ACATGGTGGT CAGCCCTGAC
    GACAGCCGCA CAGACAAGCT GGAGTCCAGT ATCTTACCGC CCCTGACTCA CGAGCTGACA
    GCCAGCGATC TGCTCATGAG CAAGATGTTC CGTGATGATG AGCTTGAAGA ATCTGAGAAA
    TTCTACGTGG ACCAGCCCCG GTTCTTGCTG CTATTCTATG CCATGTATAA CACTCTGGTA
    GCCCGCTCGG AAATGGTGTG CTATTTCGTG ATCATCCTGA ACCACATGAT CTCTGCCTCC
    ATCATTACTC TTCTGTTGCC CATCCTGATC TTCCTCTGGG CCATGCTGTC CGTCCCCAGG
    CCCAGCCGGC GCTTCTGGAT GATGGCCATC GTCTACACTG AGGTGGCGAT TGTAGTGAAA
    TATTTCTTCC AGTTTGGCTT CTTTCCTTGG AATAAGGACT TGGAAATTTA CAAGGACAGG
    CCCTACTTTC CACCAAACAT CATAGGGGTG GAAAAGAAAG AGGGCTATGT TCTTTACGAC
    CTCATTCAGC TGCTGGCTCT GTTCTTCCAT CGATCCATTC TGAAGTGTCA TGGCCTATGG
    GACGAGGATG ACATTGTTGA CAGTAACACA GACAAGGAAG GATCTGATGA TGAGCTGTCC
    CTCGGTCAGG GCAGAAGAGG CTCCTCAGAG TCACTGAAGT CCATCAACTT GGCTGCCTCT
    GTAGAGTCCG TCCATGTGAC CTTCCCAGAG CAGCCAGCAG CCATCCGGAG AAAACGCTCC
    TGCAGCAGCT CGCAGATCTC CCCACGGTCC AGCTTCTCTT CAAATAGGTC CAAAAGAGGC
    AGCACAAGCA CCCGAAACAG CAGCCAAAAA GGAAGCAGTG TTTTGAGCAT TAAACAAAAA
    AGCAAAAGAG AACTTTACAT GGAAAAGCTT CAAGAACATT TAATCAAAGC AAAGGCCTTT
    ACCATAAAGA AAACTCTACA AATCTATGTA CCCATTCGGC AATTCTTCTA TGACCTCATC
    CACCCAGACT ACAGTGCTGT GACTGACGTG TATGTGCTGA TGTTCCTGGC CGACACCGTG
    GACTTCATCA TCATCGTCTT TGGCTTCTGG GCTTTTGGGA AACACTCGGC AGCTGCAGAT
    ATCACCTCTT CCCTATCAGA AGACCAGGTC CCCGGGCCAT TCCTGGTGAT GGTTCTCATT
    CAGTTTGGAA CCATGGTGGT GGACCGTGCA CTCTACCTCA GGAAGACTGT GCTTGGCAAG
    GTCATTTTCC AGGTCATCCT CGTGTTTGGA ATTCACTTCT GGATGTTCTT CATCCTACCC
    AGTGTGACTG AGAGGAAATT CAGCCAGAAC CTGGTGGCAC AGCTCTGGTA CTTTGTGAAA
    TGTGTGTACT TTGGGTTGTC TGCCTACCAA ATCCGCTGTG GCTACCCAAC ACGTGTGCTC
    GGAAACTTCC TCACGAAGAG CTACAATTAT GTCAACCTCT TCTTGTTTCA AGGGTTTCGT
    CTCGTCCCAT TTCTGACAGA GCTGCGGGCT GTGATGGACT GGGTGTGGAC AGATACAACC
    TTGAGCCTCT CCAGCTGGAT TTGTGTGGAG GACATTTACG CACACATTTT CATCTTAAAG
    TGCTGGCGAG AGTCGGAAAA GAGATACCCT CAACCCAGGG GCCAGAAGAA GAAGAAAGCT
    GTGAAGTATG GCATGGGCGG AATGATCATT GTCCTGCTCA TCTGTATCGT CTGGTTTCCT
    CTGCTCTTCA TGTCTTTGAT CAAGTCTGTC GCAGGGGTTA TTAACCAACC CCTGGATGTG
    TCCGTCACAA TAACCCTGGG AGGCTATCAG CCTATCTTCA CAATGAGTGC CCAGCAAAGC
    CAGCTGAAAG TTATGGATGG TACAAAGTAT AACGAATTCA TAAAGTCTTT CGGCCCCAAC
    AGTGGCGCTA TGCAATTTCT GGAGAATTAT GAAAGGCAAG ACATAACAGT AGCGGAACTG
    GAAGGGAATT CGAATTCTTT GTGGACCATC AGCCCTCCCA GTAAGCAGAA AATGATACAG
    GAGCTCACCG ACCCCAATAG TTGCTTCTCT GTTGTTTTTT CATGGAGTAT CCAGAGAAAC
    ATGACTCTGG GTGCAAAGGC TGAAATAGCA ACGGACAAGC TTTCTTTTCC TCTCACGGAT
    GCGACACGAA GTAGCATAGC CAAAATGATT GCTGGCAATG ACACAGAAAG TTCCAACACA
    CCAGTGACAA TAGAAAAGAT CTACCCATAC TATGTGAAGG CACCCAGTGA CTCAAACTCA
    AAGCCTATAA AACAGCTTCT GTCAGAAAAC AATTTCATGA ATATCACCAT CATTTTGTTC
    AGAGACAATG TCACAAAGTC CAATAGTGAG TGGTGGGTTC TCAACCTGAC TGGAAGTAGG
    ATATTCAATC AGGGATCCCA AGCCTTGGAG CTGGTGGTCT TCAATGACAA AGTCAGCCCC
    CCAAGTCTGG GCTTCTTGGC TGGCTATGGC ATCATGGGAT TGTATGCATC CGTTGTCCTT
    GTAATTGGGA AGTTTGTTCG CGAATTCTTC AGTGGGATTT CTCACTCTAT CATGTTTGAA
    GAGCTTCCAA ATGTGGACAG AATTTTGAAG TTATGCACAG ATATATTTCT CGTACGGGAG
    ACAGGGGAAC TGGAGCTGGA GGAAGACCTC TATGCCAAAT TGATATTCCT GTACAGGTCG
    CCAGAAACCA TGATCAAGTG GACTAGAGAA AAAACAAATT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa54718
    Clone ID Related Accession (Same CDS sequence) XM_017601116.1 , XM_017587877.1
    Accession Version XM_017587877.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8562bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product piezo-type mechanosensitive ion channel component 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000086.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTCCCCAT CTGCCTGGCA 
    GTAGCATGTG CATTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCG ACGATGCAAG GACACACAGG GCGGCTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCCTTT CTGCTCCTTC ACATCATTTT CCACATCACA
    CTGGCCAGCC TGGAAGCTCA GCACCGCATC GCACCCGCTT ACAACTGCTC AACTTGGGAA
    AAGACCTTCC GGCAGATCGG CTTCGAAAGC TTGAAGGGAG CTGATGCTGG CAATGGCATC
    AGGGTGTTTG TGCCTGATAT CGGGATGTTC ATTGCGAGCC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACGGAA GAAGTAGCCC AATTTAATTC CGAGTGCGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GACTCAGAAG AAGCGCTGAT TTATGAGGAG
    GACTTAGATG GGGAAGAAGG CATGGAAGGC GAGCTGGAAG AAAGCACAAA GTTAAAAATG
    TTTCGCAGGC TCGCCTCCGT CGCTTCCAAG CTTAAGGAGT TCATCGGCAA TATGATAACC
    ACGGCGGGCA AGGTGGTGGT GACCATCCTG TTGGGCTCCT CTGGCATGAT GCTGCCATCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT CTGGGTCTGT GCACCTGGTG GTCCTGGTGC
    CGAACTTTTG ACCCATTGCT GTTTGGCTGC CTCTGTGTCC TGTTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTTCAGTTCT TCCAAGAAGC AGTCCCACCC
    AATGACTACT ATGCAAGGTT GTTTGGAATC AAATCCGTTA TTCAAACAGA CTGCTCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTACAC GCTGGCCACG CTGATCCGCA TTTGGTTGCA AGAGCCTCTT
    GTGCAGGAGG AGATGTCGAA GGAAGAGGAG GGTGCACTGG ATGGCTGCTC CAATCAGAAC
    ACAGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    GGCCTGCTGG TGACTGTGAA CGGCAACCCT GTGGACTACC ACACCATCCA CCCAAGTCTG
    CCCATAGAGA ATGGCCCCGC CAAGACGGAC TTGTATACTA CTCCTCAGTA CCGGTGGGAG
    CCCTCAGAGG AGTCCTCAGA AAAGAAAGAG GAGGAAGAGG ACAAGAGAGA AGACTCAGAA
    GGGGAAGGGA GCCAAGAGGA AAAACGGAGT GTGAGAATTC ATGCCATGGT CGCCGTGTTC
    CAGTTCATCA TGAAGCAGAG TTACATCTGT GCCCTCATCG CCATGATGGC ATGGAGCATC
    ACCTACCACA GCTGGCTGAC ATTTGTGCTG TTGATCTGGT CGTGCACTCT CTGGATGATT
    CGCAACAGGA GGAAGTACGC CATGATCAGC TCTCCGTTCA TGGTTGTCTA TGCCAATCTG
    CTGCTGGTAC TGCAGTACAT ATGGAGCTTT GAACTTCCAG AAATCAAGAA AGTCCCTGGA
    TTTCTAGAAA AGAAAGAGCC TGGAGAGCTT GCCTCAAAGA TCCTCTTCAC CATCACATTT
    TGGCTGCTCC TGAGGCAGCA TCTCACAGAG CAGAAGGCCC TTCAGGAAAA GGAAGCACTT
    CTGTCTGAAG TCAAAATTGG AAGTCAAGAA CTTGACGAAA AGGATGAGGA ACTCCAAGAT
    ATACAAGTGG AAGGAGAGCC CATAGAGAAG GAAGAAGAGG AGGAGGAAAT AAAGGAAGAC
    AGACATGAGG TAAAGAAGGA AGAGGAAGAG GAAGAGGAAG ATGACGATGA CCAGGACATC
    ATGAAGGTTC TGGGCAATCT GGTGGTGGCC CTCTTCATCA AGTATTGGAT CTACGTCTGC
    GGGGGCATGT TCTTTTTTGT CAGCTTTGAG GGGAAAATCG TCATGTACAA AATCATCTAC
    ATGGTGCTGT TCCTGTTCTG TGTGGCCTTG TACCAGGTGC ACTACGAGTG GTGGAGGAAG
    ATCCTGAAAT ACTTCTGGAT GTCGGTGGTC ATTTACACCA TGCTGGTGCT CATCTTCATC
    TACACTTACC AGTTTGAGAA CTTCCCAGGC CTGTGGCAAA ACATGACCGG GTTAAAGAAA
    GAGAAGCTTG AGGATCTGGG CCTGAAGCAG TTCACTGTGG CCGAACTGTT CACACGAATA
    TTCATCCCCA CCTCCTTTCT GCTGGTCTGC ATCCTGCACC TCCACTATTT CCATGACCGG
    TTCCTGGAAC TCACAGACCT CAAGTCCATC CCCAGCAAAG AAGACAACAC CATCTACAGC
    CATGCCAAGG TCAATGGTCG CGTGTACCTA ATAATAAATA GCATCAAGAA AAAATTACCA
    ATCCACCAGA ATGAGTTAGC CCACCCTGAA GGAAGCCTCC CAGACCTAGC CATTATGAAC
    ATGACGGCCA GCCTCGATAA GCCTGAGAGG CAGAAGTTGG CAGAGCCTGG GGAGGAGAGG
    ACAGAAGAAT GTGCCAAGAA GGCAGAGAAG GGTGAGGCTG GGAAAGACAG TGATGAGTCT
    GAGGAGGAGG AAGGTGAGGA AGAGGAATCC GAGGAGGAAG AATCCTCAGA TCTCAGGAAC
    AAGTGGCACC TCGTGATTGA CCGGCTCACT GTGCTCTTTC TAAAGTTCCT GGAGTATTTC
    CACAAGTTGC AGGTGTTTAT GTGGTGGATC TTGGAGCTGC ACATCATCAA AATCGTCTCA
    TCGTACATTA TCTGGGTGAC TGTCAAAGAG GTGTCTCTGT TCAACTATGT ATTTTTGATT
    TCTTGGGCAT TTGCTCTGCC ATATGCCAAA CTCCGCCGTG TGGCTTCCAG TGTCTGCACT
    GTCTGGACGT GTGTGATCAT CGTCTGCAAA ATGTTGTACC AGCTGCAAAC CATTAAGCCT
    GAGAACTTTT CTGTCAACTG CTCCTTGCCC AATGAAAATC AAACCAACAT ACCCCTTAAT
    GAGTTGAACA AGTCTCTTCT CTACAGTGCC CCTGTTGATC CCACTGAGTG GGTTGGCCTG
    AGGAAGTCCT CCCCTTTACT CGTCTACCTG AGGAACAACC TCCTGATGTT GGCGATTTTG
    GCCTTCGAAG TCACTGTTTA CCGACATCAG GAGTACTATC GAGGCCGAAA CAACTTGACT
    GCTCCGGTGT CTAAAACTAT CTTTCATGAC ATCACAAGGT TACATCTAGA TGATGGGCTT
    ATCAATTGTG CCAAATATTT TGTAAACTAC TTCTTCTACA AGTTTGGCCT AGAGACCTGT
    TTCCTAATGT CAGTGAACGT AATTGGACAG CGAATGGACT TCTACGCCAT GATTCATGCC
    TGTTGGCTGA TCGGTGTCTT GTACCGTCGC AGAAGAAAAG CCATTGCTGA GGTCTGGCCC
    AAGTACTGCT GCTTCCTGGC TTGCATCATC ACCTTCCAGT ACTTTGTCTG CATAGGCATC
    CCTCCTGCCC CCTGCCGAGA TTACCCATGG AGGTTCAAGG GTGCCAACTT CAATGACAAC
    ATCATCAAGT GGCTGTACTT CCCAGATTTC ATCGTGCGGC CCAACCCTGT GTTTCTTGTC
    TATGACTTCA TGCTGCTCCT GTGTGCTTCC TTACAACGAC AGATTTTTGA GGACGAAAAC
    AAGGCAGCTG TGCGCATCAT GGCCGGGGAC AATGTCGAGA TTTGCATGAA CCTTGACGCG
    GCCTCATTCA GCCAGCATAA CCCTGTACCA GATTTCATTC ACTGCAGATC TTACTTGGAC
    ATGTCCAAAG TGATCATCTT CAGCTACCTC TTCTGGTTCG TCCTCACCAT CATCTTCATT
    ACCGGAACGA CCAGGATCAG CATCTTTTGC ATGGGTTACC TGGTGGCCTG TTTCTATTTC
    CTGCTCTTTG GAGGTGATCT GCTCCTGAAA CCCATCAAGA GCATCCTACG CTACTGGGAC
    TGGCTGATTG CATACAACGT GTTTGTGATC ACCATGAAAA ACATACTGTC AATAGGAGCC
    TGTGGATACA TCGGTGCACT GGTGAGAAAC AGCTGCTGGC TGATCCAAGC GTTCAGCCTG
    GCCTGCACGG TGAAAGGCTA TAAAATGCCT GAAGATGACT CCAGATGCAA GCTGCCCAGT
    GGGGAGGCAG GGATCATCTG GGACAGCATC TGCTTTGCGT TTCTCCTGCT ACAGAGGAGA
    GTCTTCATGA GTTACTATTT CCTGCATGTC GTGGCTGACA TTAAAGCATC CCAGATCCTG
    GCATCCAGAG GGGCCGAACT TTTCCAGGCT ACAATTGTGA AAGCTGTAAA GGCAAGAATT
    GAAGAAGAGA AAAAATCCAT GGACCAGCTG AAGAGACAGA TGGATCGCAT CAAAGCCCGG
    CAGCAAAAGT ACAAAAAGGG TAAGGAGAGG ATGCTGAGCT TGACCCAGGA GTCAGGGGAA
    GGCCAGGACA TCCAGAAAGT CTCAGAAGAA GACGATGAAA GAGAAGCAGA CAAACAGAAA
    GCCAAGGGCA AAAAAAAGCA GTGGTGGCGG CCTTGGGTTG ATCATGCTTC CATGGTCAGG
    AGTGGAGATT ATTATTTGTT TGAAACGGAT AGTGAAGAGG AGGAAGAAGA GGAACTCAAA
    AAGGAGGATG AAGAACCACC ACGGAAGTCT GCATTCCAGA GAGCTATTGG GAAGTTTGCC
    TCAGCCATTT TAGCCTTGCC AAAGTCTGTC ATTAAACTTC CCAAAACTAT TCTTCAGTAT
    TTAATCAGAG CTGCAAAGTT CGTCTACCAA GCCTGGATTA CCGATCCTAA AACAGCACTC
    CGACAGAGGA GGAAAGAGAA GAAGAAAATG GCCAGAGAAG AGCAGAAGGG AAGACGCAAA
    GGATCTGGGG AGGGTCCAGT GGAATGGGAA GACAGAGAAG ATGAGCCAAT CAAAAAGAAA
    TCTGATGGAC CAGATAATAT CATCAAGAGG ATATTTAATA TCCTGAAGTT CACCTGGGTC
    CTGTTCCTGG CCACAGTGGA CAGTTTCACT ACTTGGCTCA ACTCCATCTC AAGAGAGCAT
    ATCGACATAT CCACAGTTCT GCGGATTGAA AGATGCATGC TGACCCGAGA GATAAAGAAG
    GGCAACGTTC CGACACGGGA AAGCATCCAC ATGTACTACC AGAACCACAT GATGAACCTT
    TCCAGAGAAT CCGGACTGGA CACAATCGAC GAGCACTCTG GCGCTGGTTC CAGGGCCCAG
    GCAGCACACA GGATGGATAG CTTAGATTCC CATGACAGTA TCTCCAGCTG TTACACTGAA
    GCAACCCTGC TGATCTCAAG GCAGTCCACC CTTGATGATT TAGATGGACA AGACCCTGTT
    CCTAAAACAA GCGAGCGTGC TCGGCCTAGG CTCCGTAAAG TGTTCAGCAT GGACATGTCC
    TCCTCATCAG CTGACAGTGG CAGTGTAGCA TCAAGCGAGC CCACACAGTG CACCATGCTG
    TATTCACGCC AAGGAACTAC GGAAACCATT GAGGAGGTAG AGGCTGAAGC TGAGGAGGAG
    GTGGTAGAGA GTCTGGAGCC TGAGCTCCAT GATGCCGAAG AGAAGGAATA TGCAGCTGAC
    TATGAAGCAG GGGTAGAGGA GGTCAGCCTC ACTCCAGATG AGGAGTTGCC TCAGTTCTCA
    ACAGATGACG GTGACGTGGA GGCCCCGCCC TCCTACAGCA AGGCTGTCAG CTTTGAGCAC
    CTCTCGTTTG CATCACAAGA TGACTCCGGG GGCAAGAACC ACATGGTGGT CAGCCCTGAC
    GACAGCCGCA CAGACAAGCT GGAGTCCAGT ATCTTACCGC CCCTGACTCA CGAGCTGACA
    GCCAGCGATC TGCTCATGAG CAAGATGTTC CGTGATGATG AGCTTGAAGA ATCTGAGAAA
    TTCTACGTGG ACCAGCCCCG GTTCTTGCTG CTATTCTATG CCATGTATAA CACTCTGGTA
    GCCCGCTCGG AAATGGTGTG CTATTTCGTG ATCATCCTGA ACCACATGAT CTCTGCCTCC
    ATCATTACTC TTCTGTTGCC CATCCTGATC TTCCTCTGGG CCATGCTGTC CGTCCCCAGG
    CCCAGCCGGC GCTTCTGGAT GATGGCCATC GTCTACACTG AGGTGGCGAT TGTAGTGAAA
    TATTTCTTCC AGTTTGGCTT CTTTCCTTGG AATAAGGACT TGGAAATTTA CAAGGACAGG
    CCCTACTTTC CACCAAACAT CATAGGGGTG GAAAAGAAAG AGGGCTATGT TCTTTACGAC
    CTCATTCAGC TGCTGGCTCT GTTCTTCCAT CGATCCATTC TGAAGTGTCA TGGCCTATGG
    GACGAGGATG ACATTGTTGA CAGTAACACA GACAAGGAAG GATCTGATGA TGAGCTGTCC
    CTCGGTCAGG GCAGAAGAGG CTCCTCAGAG TCACTGAAGT CCATCAACTT GGCTGCCTCT
    GTAGAGTCCG TCCATGTGAC CTTCCCAGAG CAGCCAGCAG CCATCCGGAG AAAACGCTCC
    TGCAGCAGCT CGCAGATCTC CCCACGGTCC AGCTTCTCTT CAAATAGGTC CAAAAGAGGC
    AGCACAAGCA CCCGAAACAG CAGCCAAAAA GGAAGCAGTG TTTTGAGCAT TAAACAAAAA
    AGCAAAAGAG AACTTTACAT GGAAAAGCTT CAAGAACATT TAATCAAAGC AAAGGCCTTT
    ACCATAAAGA AAACTCTACA AATCTATGTA CCCATTCGGC AATTCTTCTA TGACCTCATC
    CACCCAGACT ACAGTGCTGT GACTGACGTG TATGTGCTGA TGTTCCTGGC CGACACCGTG
    GACTTCATCA TCATCGTCTT TGGCTTCTGG GCTTTTGGGA AACACTCGGC AGCTGCAGAT
    ATCACCTCTT CCCTATCAGA AGACCAGGTC CCCGGGCCAT TCCTGGTGAT GGTTCTCATT
    CAGTTTGGAA CCATGGTGGT GGACCGTGCA CTCTACCTCA GGAAGACTGT GCTTGGCAAG
    GTCATTTTCC AGGTCATCCT CGTGTTTGGA ATTCACTTCT GGATGTTCTT CATCCTACCC
    AGTGTGACTG AGAGGAAATT CAGCCAGAAC CTGGTGGCAC AGCTCTGGTA CTTTGTGAAA
    TGTGTGTACT TTGGGTTGTC TGCCTACCAA ATCCGCTGTG GCTACCCAAC ACGTGTGCTC
    GGAAACTTCC TCACGAAGAG CTACAATTAT GTCAACCTCT TCTTGTTTCA AGGGTTTCGT
    CTCGTCCCAT TTCTGACAGA GCTGCGGGCT GTGATGGACT GGGTGTGGAC AGATACAACC
    TTGAGCCTCT CCAGCTGGAT TTGTGTGGAG GACATTTACG CACACATTTT CATCTTAAAG
    TGCTGGCGAG AGTCGGAAAA GAGATACCCT CAACCCAGGG GCCAGAAGAA GAAGAAAGCT
    GTGAAGTATG GCATGGGCGG AATGATCATT GTCCTGCTCA TCTGTATCGT CTGGTTTCCT
    CTGCTCTTCA TGTCTTTGAT CAAGTCTGTC GCAGGGGTTA TTAACCAACC CCTGGATGTG
    TCCGTCACAA TAACCCTGGG AGGCTATCAG CCTATCTTCA CAATGAGTGC CCAGCAAAGC
    CAGCTGAAAG TTATGGATGG TACAAAGTAT AACGAATTCA TAAAGTCTTT CGGCCCCAAC
    AGTGGCGCTA TGCAATTTCT GGAGAATTAT GAAAGGCAAG ACATAACAGT AGCGGAACTG
    GAAGGGAATT CGAATTCTTT GTGGACCATC AGCCCTCCCA GTAAGCAGAA AATGATACAG
    GAGCTCACCG ACCCCAATAG TTGCTTCTCT GTTGTTTTTT CATGGAGTAT CCAGAGAAAC
    ATGACTCTGG GTGCAAAGGC TGAAATAGCA ACGGACAAGC TTTCTTTTCC TCTCACGGAT
    GCGACACGAA GTAGCATAGC CAAAATGATT GCTGGCAATG ACACAGAAAG TTCCAACACA
    CCAGTGACAA TAGAAAAGAT CTACCCATAC TATGTGAAGG CACCCAGTGA CTCAAACTCA
    AAGCCTATAA AACAGCTTCT GTCAGAAAAC AATTTCATGA ATATCACCAT CATTTTGTTC
    AGAGACAATG TCACAAAGTC CAATAGTGAG TGGTGGGTTC TCAACCTGAC TGGAAGTAGG
    ATATTCAATC AGGGATCCCA AGCCTTGGAG CTGGTGGTCT TCAATGACAA AGTCAGCCCC
    CCAAGTCTGG GCTTCTTGGC TGGCTATGGC ATCATGGGAT TGTATGCATC CGTTGTCCTT
    GTAATTGGGA AGTTTGTTCG CGAATTCTTC AGTGGGATTT CTCACTCTAT CATGTTTGAA
    GAGCTTCCAA ATGTGGACAG AATTTTGAAG TTATGCACAG ATATATTTCT CGTACGGGAG
    ACAGGGGAAC TGGAGCTGGA GGAAGACCTC TATGCCAAAT TGATATTCCT GTACAGGTCG
    CCAGAAACCA TGATCAAGTG GACTAGAGAA AAAACAAATT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017443366.1
    CDS940..9501
    Translation

    Target ORF information:

    RefSeq Version XM_017587877.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017587877.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTCCCCAT CTGCCTGGCA 
    GTAGCATGTG CATTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCG ACGATGCAAG GACACACAGG GCGGCTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCCTTT CTGCTCCTTC ACATCATTTT CCACATCACA
    CTGGCCAGCC TGGAAGCTCA GCACCGCATC GCACCCGCTT ACAACTGCTC AACTTGGGAA
    AAGACCTTCC GGCAGATCGG CTTCGAAAGC TTGAAGGGAG CTGATGCTGG CAATGGCATC
    AGGGTGTTTG TGCCTGATAT CGGGATGTTC ATTGCGAGCC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACGGAA GAAGTAGCCC AATTTAATTC CGAGTGCGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GACTCAGAAG AAGCGCTGAT TTATGAGGAG
    GACTTAGATG GGGAAGAAGG CATGGAAGGC GAGCTGGAAG AAAGCACAAA GTTAAAAATG
    TTTCGCAGGC TCGCCTCCGT CGCTTCCAAG CTTAAGGAGT TCATCGGCAA TATGATAACC
    ACGGCGGGCA AGGTGGTGGT GACCATCCTG TTGGGCTCCT CTGGCATGAT GCTGCCATCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT CTGGGTCTGT GCACCTGGTG GTCCTGGTGC
    CGAACTTTTG ACCCATTGCT GTTTGGCTGC CTCTGTGTCC TGTTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTTCAGTTCT TCCAAGAAGC AGTCCCACCC
    AATGACTACT ATGCAAGGTT GTTTGGAATC AAATCCGTTA TTCAAACAGA CTGCTCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTACAC GCTGGCCACG CTGATCCGCA TTTGGTTGCA AGAGCCTCTT
    GTGCAGGAGG AGATGTCGAA GGAAGAGGAG GGTGCACTGG ATGGCTGCTC CAATCAGAAC
    ACAGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    GGCCTGCTGG TGACTGTGAA CGGCAACCCT GTGGACTACC ACACCATCCA CCCAAGTCTG
    CCCATAGAGA ATGGCCCCGC CAAGACGGAC TTGTATACTA CTCCTCAGTA CCGGTGGGAG
    CCCTCAGAGG AGTCCTCAGA AAAGAAAGAG GAGGAAGAGG ACAAGAGAGA AGACTCAGAA
    GGGGAAGGGA GCCAAGAGGA AAAACGGAGT GTGAGAATTC ATGCCATGGT CGCCGTGTTC
    CAGTTCATCA TGAAGCAGAG TTACATCTGT GCCCTCATCG CCATGATGGC ATGGAGCATC
    ACCTACCACA GCTGGCTGAC ATTTGTGCTG TTGATCTGGT CGTGCACTCT CTGGATGATT
    CGCAACAGGA GGAAGTACGC CATGATCAGC TCTCCGTTCA TGGTTGTCTA TGCCAATCTG
    CTGCTGGTAC TGCAGTACAT ATGGAGCTTT GAACTTCCAG AAATCAAGAA AGTCCCTGGA
    TTTCTAGAAA AGAAAGAGCC TGGAGAGCTT GCCTCAAAGA TCCTCTTCAC CATCACATTT
    TGGCTGCTCC TGAGGCAGCA TCTCACAGAG CAGAAGGCCC TTCAGGAAAA GGAAGCACTT
    CTGTCTGAAG TCAAAATTGG AAGTCAAGAA CTTGACGAAA AGGATGAGGA ACTCCAAGAT
    ATACAAGTGG AAGGAGAGCC CATAGAGAAG GAAGAAGAGG AGGAGGAAAT AAAGGAAGAC
    AGACATGAGG TAAAGAAGGA AGAGGAAGAG GAAGAGGAAG ATGACGATGA CCAGGACATC
    ATGAAGGTTC TGGGCAATCT GGTGGTGGCC CTCTTCATCA AGTATTGGAT CTACGTCTGC
    GGGGGCATGT TCTTTTTTGT CAGCTTTGAG GGGAAAATCG TCATGTACAA AATCATCTAC
    ATGGTGCTGT TCCTGTTCTG TGTGGCCTTG TACCAGGTGC ACTACGAGTG GTGGAGGAAG
    ATCCTGAAAT ACTTCTGGAT GTCGGTGGTC ATTTACACCA TGCTGGTGCT CATCTTCATC
    TACACTTACC AGTTTGAGAA CTTCCCAGGC CTGTGGCAAA ACATGACCGG GTTAAAGAAA
    GAGAAGCTTG AGGATCTGGG CCTGAAGCAG TTCACTGTGG CCGAACTGTT CACACGAATA
    TTCATCCCCA CCTCCTTTCT GCTGGTCTGC ATCCTGCACC TCCACTATTT CCATGACCGG
    TTCCTGGAAC TCACAGACCT CAAGTCCATC CCCAGCAAAG AAGACAACAC CATCTACAGC
    CATGCCAAGG TCAATGGTCG CGTGTACCTA ATAATAAATA GCATCAAGAA AAAATTACCA
    ATCCACCAGA ATGAGTTAGC CCACCCTGAA GGAAGCCTCC CAGACCTAGC CATTATGAAC
    ATGACGGCCA GCCTCGATAA GCCTGAGAGG CAGAAGTTGG CAGAGCCTGG GGAGGAGAGG
    ACAGAAGAAT GTGCCAAGAA GGCAGAGAAG GGTGAGGCTG GGAAAGACAG TGATGAGTCT
    GAGGAGGAGG AAGGTGAGGA AGAGGAATCC GAGGAGGAAG AATCCTCAGA TCTCAGGAAC
    AAGTGGCACC TCGTGATTGA CCGGCTCACT GTGCTCTTTC TAAAGTTCCT GGAGTATTTC
    CACAAGTTGC AGGTGTTTAT GTGGTGGATC TTGGAGCTGC ACATCATCAA AATCGTCTCA
    TCGTACATTA TCTGGGTGAC TGTCAAAGAG GTGTCTCTGT TCAACTATGT ATTTTTGATT
    TCTTGGGCAT TTGCTCTGCC ATATGCCAAA CTCCGCCGTG TGGCTTCCAG TGTCTGCACT
    GTCTGGACGT GTGTGATCAT CGTCTGCAAA ATGTTGTACC AGCTGCAAAC CATTAAGCCT
    GAGAACTTTT CTGTCAACTG CTCCTTGCCC AATGAAAATC AAACCAACAT ACCCCTTAAT
    GAGTTGAACA AGTCTCTTCT CTACAGTGCC CCTGTTGATC CCACTGAGTG GGTTGGCCTG
    AGGAAGTCCT CCCCTTTACT CGTCTACCTG AGGAACAACC TCCTGATGTT GGCGATTTTG
    GCCTTCGAAG TCACTGTTTA CCGACATCAG GAGTACTATC GAGGCCGAAA CAACTTGACT
    GCTCCGGTGT CTAAAACTAT CTTTCATGAC ATCACAAGGT TACATCTAGA TGATGGGCTT
    ATCAATTGTG CCAAATATTT TGTAAACTAC TTCTTCTACA AGTTTGGCCT AGAGACCTGT
    TTCCTAATGT CAGTGAACGT AATTGGACAG CGAATGGACT TCTACGCCAT GATTCATGCC
    TGTTGGCTGA TCGGTGTCTT GTACCGTCGC AGAAGAAAAG CCATTGCTGA GGTCTGGCCC
    AAGTACTGCT GCTTCCTGGC TTGCATCATC ACCTTCCAGT ACTTTGTCTG CATAGGCATC
    CCTCCTGCCC CCTGCCGAGA TTACCCATGG AGGTTCAAGG GTGCCAACTT CAATGACAAC
    ATCATCAAGT GGCTGTACTT CCCAGATTTC ATCGTGCGGC CCAACCCTGT GTTTCTTGTC
    TATGACTTCA TGCTGCTCCT GTGTGCTTCC TTACAACGAC AGATTTTTGA GGACGAAAAC
    AAGGCAGCTG TGCGCATCAT GGCCGGGGAC AATGTCGAGA TTTGCATGAA CCTTGACGCG
    GCCTCATTCA GCCAGCATAA CCCTGTACCA GATTTCATTC ACTGCAGATC TTACTTGGAC
    ATGTCCAAAG TGATCATCTT CAGCTACCTC TTCTGGTTCG TCCTCACCAT CATCTTCATT
    ACCGGAACGA CCAGGATCAG CATCTTTTGC ATGGGTTACC TGGTGGCCTG TTTCTATTTC
    CTGCTCTTTG GAGGTGATCT GCTCCTGAAA CCCATCAAGA GCATCCTACG CTACTGGGAC
    TGGCTGATTG CATACAACGT GTTTGTGATC ACCATGAAAA ACATACTGTC AATAGGAGCC
    TGTGGATACA TCGGTGCACT GGTGAGAAAC AGCTGCTGGC TGATCCAAGC GTTCAGCCTG
    GCCTGCACGG TGAAAGGCTA TAAAATGCCT GAAGATGACT CCAGATGCAA GCTGCCCAGT
    GGGGAGGCAG GGATCATCTG GGACAGCATC TGCTTTGCGT TTCTCCTGCT ACAGAGGAGA
    GTCTTCATGA GTTACTATTT CCTGCATGTC GTGGCTGACA TTAAAGCATC CCAGATCCTG
    GCATCCAGAG GGGCCGAACT TTTCCAGGCT ACAATTGTGA AAGCTGTAAA GGCAAGAATT
    GAAGAAGAGA AAAAATCCAT GGACCAGCTG AAGAGACAGA TGGATCGCAT CAAAGCCCGG
    CAGCAAAAGT ACAAAAAGGG TAAGGAGAGG ATGCTGAGCT TGACCCAGGA GTCAGGGGAA
    GGCCAGGACA TCCAGAAAGT CTCAGAAGAA GACGATGAAA GAGAAGCAGA CAAACAGAAA
    GCCAAGGGCA AAAAAAAGCA GTGGTGGCGG CCTTGGGTTG ATCATGCTTC CATGGTCAGG
    AGTGGAGATT ATTATTTGTT TGAAACGGAT AGTGAAGAGG AGGAAGAAGA GGAACTCAAA
    AAGGAGGATG AAGAACCACC ACGGAAGTCT GCATTCCAGA GAGCTATTGG GAAGTTTGCC
    TCAGCCATTT TAGCCTTGCC AAAGTCTGTC ATTAAACTTC CCAAAACTAT TCTTCAGTAT
    TTAATCAGAG CTGCAAAGTT CGTCTACCAA GCCTGGATTA CCGATCCTAA AACAGCACTC
    CGACAGAGGA GGAAAGAGAA GAAGAAAATG GCCAGAGAAG AGCAGAAGGG AAGACGCAAA
    GGATCTGGGG AGGGTCCAGT GGAATGGGAA GACAGAGAAG ATGAGCCAAT CAAAAAGAAA
    TCTGATGGAC CAGATAATAT CATCAAGAGG ATATTTAATA TCCTGAAGTT CACCTGGGTC
    CTGTTCCTGG CCACAGTGGA CAGTTTCACT ACTTGGCTCA ACTCCATCTC AAGAGAGCAT
    ATCGACATAT CCACAGTTCT GCGGATTGAA AGATGCATGC TGACCCGAGA GATAAAGAAG
    GGCAACGTTC CGACACGGGA AAGCATCCAC ATGTACTACC AGAACCACAT GATGAACCTT
    TCCAGAGAAT CCGGACTGGA CACAATCGAC GAGCACTCTG GCGCTGGTTC CAGGGCCCAG
    GCAGCACACA GGATGGATAG CTTAGATTCC CATGACAGTA TCTCCAGCTG TTACACTGAA
    GCAACCCTGC TGATCTCAAG GCAGTCCACC CTTGATGATT TAGATGGACA AGACCCTGTT
    CCTAAAACAA GCGAGCGTGC TCGGCCTAGG CTCCGTAAAG TGTTCAGCAT GGACATGTCC
    TCCTCATCAG CTGACAGTGG CAGTGTAGCA TCAAGCGAGC CCACACAGTG CACCATGCTG
    TATTCACGCC AAGGAACTAC GGAAACCATT GAGGAGGTAG AGGCTGAAGC TGAGGAGGAG
    GTGGTAGAGA GTCTGGAGCC TGAGCTCCAT GATGCCGAAG AGAAGGAATA TGCAGCTGAC
    TATGAAGCAG GGGTAGAGGA GGTCAGCCTC ACTCCAGATG AGGAGTTGCC TCAGTTCTCA
    ACAGATGACG GTGACGTGGA GGCCCCGCCC TCCTACAGCA AGGCTGTCAG CTTTGAGCAC
    CTCTCGTTTG CATCACAAGA TGACTCCGGG GGCAAGAACC ACATGGTGGT CAGCCCTGAC
    GACAGCCGCA CAGACAAGCT GGAGTCCAGT ATCTTACCGC CCCTGACTCA CGAGCTGACA
    GCCAGCGATC TGCTCATGAG CAAGATGTTC CGTGATGATG AGCTTGAAGA ATCTGAGAAA
    TTCTACGTGG ACCAGCCCCG GTTCTTGCTG CTATTCTATG CCATGTATAA CACTCTGGTA
    GCCCGCTCGG AAATGGTGTG CTATTTCGTG ATCATCCTGA ACCACATGAT CTCTGCCTCC
    ATCATTACTC TTCTGTTGCC CATCCTGATC TTCCTCTGGG CCATGCTGTC CGTCCCCAGG
    CCCAGCCGGC GCTTCTGGAT GATGGCCATC GTCTACACTG AGGTGGCGAT TGTAGTGAAA
    TATTTCTTCC AGTTTGGCTT CTTTCCTTGG AATAAGGACT TGGAAATTTA CAAGGACAGG
    CCCTACTTTC CACCAAACAT CATAGGGGTG GAAAAGAAAG AGGGCTATGT TCTTTACGAC
    CTCATTCAGC TGCTGGCTCT GTTCTTCCAT CGATCCATTC TGAAGTGTCA TGGCCTATGG
    GACGAGGATG ACATTGTTGA CAGTAACACA GACAAGGAAG GATCTGATGA TGAGCTGTCC
    CTCGGTCAGG GCAGAAGAGG CTCCTCAGAG TCACTGAAGT CCATCAACTT GGCTGCCTCT
    GTAGAGTCCG TCCATGTGAC CTTCCCAGAG CAGCCAGCAG CCATCCGGAG AAAACGCTCC
    TGCAGCAGCT CGCAGATCTC CCCACGGTCC AGCTTCTCTT CAAATAGGTC CAAAAGAGGC
    AGCACAAGCA CCCGAAACAG CAGCCAAAAA GGAAGCAGTG TTTTGAGCAT TAAACAAAAA
    AGCAAAAGAG AACTTTACAT GGAAAAGCTT CAAGAACATT TAATCAAAGC AAAGGCCTTT
    ACCATAAAGA AAACTCTACA AATCTATGTA CCCATTCGGC AATTCTTCTA TGACCTCATC
    CACCCAGACT ACAGTGCTGT GACTGACGTG TATGTGCTGA TGTTCCTGGC CGACACCGTG
    GACTTCATCA TCATCGTCTT TGGCTTCTGG GCTTTTGGGA AACACTCGGC AGCTGCAGAT
    ATCACCTCTT CCCTATCAGA AGACCAGGTC CCCGGGCCAT TCCTGGTGAT GGTTCTCATT
    CAGTTTGGAA CCATGGTGGT GGACCGTGCA CTCTACCTCA GGAAGACTGT GCTTGGCAAG
    GTCATTTTCC AGGTCATCCT CGTGTTTGGA ATTCACTTCT GGATGTTCTT CATCCTACCC
    AGTGTGACTG AGAGGAAATT CAGCCAGAAC CTGGTGGCAC AGCTCTGGTA CTTTGTGAAA
    TGTGTGTACT TTGGGTTGTC TGCCTACCAA ATCCGCTGTG GCTACCCAAC ACGTGTGCTC
    GGAAACTTCC TCACGAAGAG CTACAATTAT GTCAACCTCT TCTTGTTTCA AGGGTTTCGT
    CTCGTCCCAT TTCTGACAGA GCTGCGGGCT GTGATGGACT GGGTGTGGAC AGATACAACC
    TTGAGCCTCT CCAGCTGGAT TTGTGTGGAG GACATTTACG CACACATTTT CATCTTAAAG
    TGCTGGCGAG AGTCGGAAAA GAGATACCCT CAACCCAGGG GCCAGAAGAA GAAGAAAGCT
    GTGAAGTATG GCATGGGCGG AATGATCATT GTCCTGCTCA TCTGTATCGT CTGGTTTCCT
    CTGCTCTTCA TGTCTTTGAT CAAGTCTGTC GCAGGGGTTA TTAACCAACC CCTGGATGTG
    TCCGTCACAA TAACCCTGGG AGGCTATCAG CCTATCTTCA CAATGAGTGC CCAGCAAAGC
    CAGCTGAAAG TTATGGATGG TACAAAGTAT AACGAATTCA TAAAGTCTTT CGGCCCCAAC
    AGTGGCGCTA TGCAATTTCT GGAGAATTAT GAAAGGCAAG ACATAACAGT AGCGGAACTG
    GAAGGGAATT CGAATTCTTT GTGGACCATC AGCCCTCCCA GTAAGCAGAA AATGATACAG
    GAGCTCACCG ACCCCAATAG TTGCTTCTCT GTTGTTTTTT CATGGAGTAT CCAGAGAAAC
    ATGACTCTGG GTGCAAAGGC TGAAATAGCA ACGGACAAGC TTTCTTTTCC TCTCACGGAT
    GCGACACGAA GTAGCATAGC CAAAATGATT GCTGGCAATG ACACAGAAAG TTCCAACACA
    CCAGTGACAA TAGAAAAGAT CTACCCATAC TATGTGAAGG CACCCAGTGA CTCAAACTCA
    AAGCCTATAA AACAGCTTCT GTCAGAAAAC AATTTCATGA ATATCACCAT CATTTTGTTC
    AGAGACAATG TCACAAAGTC CAATAGTGAG TGGTGGGTTC TCAACCTGAC TGGAAGTAGG
    ATATTCAATC AGGGATCCCA AGCCTTGGAG CTGGTGGTCT TCAATGACAA AGTCAGCCCC
    CCAAGTCTGG GCTTCTTGGC TGGCTATGGC ATCATGGGAT TGTATGCATC CGTTGTCCTT
    GTAATTGGGA AGTTTGTTCG CGAATTCTTC AGTGGGATTT CTCACTCTAT CATGTTTGAA
    GAGCTTCCAA ATGTGGACAG AATTTTGAAG TTATGCACAG ATATATTTCT CGTACGGGAG
    ACAGGGGAAC TGGAGCTGGA GGAAGACCTC TATGCCAAAT TGATATTCCT GTACAGGTCG
    CCAGAAACCA TGATCAAGTG GACTAGAGAA AAAACAAATT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa54719
    Clone ID Related Accession (Same CDS sequence) XM_017587878.1 , XM_017601117.1
    Accession Version XM_017587878.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8529bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product piezo-type mechanosensitive ion channel component 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000086.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
    241
    301
    361
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    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
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    2401
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    2521
    2581
    2641
    2701
    2761
    2821
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    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
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    4081
    4141
    4201
    4261
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    4381
    4441
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    4681
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    4921
    4981
    5041
    5101
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    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
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    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
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    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTCCCCAT CTGCCTGGCA 
    GTAGCATGTG CATTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCG ACGATGCAAG GACACACAGG GCGGCTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCCTTT CTGCTCCTTC ACATCATTTT CCACATCACA
    CTGGCCAGCC TGGAAGCTCA GCACCGCATC GCACCCGCTT ACAACTGCTC AACTTGGGAA
    AAGACCTTCC GGCAGATCGG CTTCGAAAGC TTGAAGGGAG CTGATGCTGG CAATGGCATC
    AGGGTGTTTG TGCCTGATAT CGGGATGTTC ATTGCGAGCC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACGGAA GAAGTAGCCC AATTTAATTC CGAGTGCGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GACTCAGAAG AAGCGCTGAT TTATGAGGAG
    GACTTAGATG GGGAAGAAGG CATGGAAGGC GAGCTGGAAG AAAGCACAAA GTTAAAAATG
    TTTCGCAGGC TCGCCTCCGT CGCTTCCAAG CTTAAGGAGT TCATCGGCAA TATGATAACC
    ACGGCGGGCA AGGTGGTGGT GACCATCCTG TTGGGCTCCT CTGGCATGAT GCTGCCATCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT CTGGGTCTGT GCACCTGGTG GTCCTGGTGC
    CGAACTTTTG ACCCATTGCT GTTTGGCTGC CTCTGTGTCC TGTTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTTCAGTTCT TCCAAGAAGC AGTCCCACCC
    AATGACTACT ATGCAAGGTT GTTTGGAATC AAATCCGTTA TTCAAACAGA CTGCTCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTACAC GCTGGCCACG CTGATCCGCA TTTGGTTGCA AGAGCCTCTT
    GTGCAGGAGG AGATGTCGAA GGAAGAGGAG GGTGCACTGG ATGGCTGCTC CAATCAGAAC
    ACAGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    CTTTTATCCA TGACCCAAGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACTGTGAAC
    GGCAACCCTG TGGACTACCA CACCATCCAC CCAAGTCTGC CCATAGAGAA TGGCCCCGCC
    AAGACGGACT TGTATACTAC TCCTCAGTAC CGGTGGGAGC CCTCAGAGGA GTCCTCAGAA
    AAGAAAGAGG AGGAAGAGGA CAAGAGAGAA GACTCAGAAG GGGAAGGGAG CCAAGAGGAA
    AAACGGAGTG TGAGAATTCA TGCCATGGTC GCCGTGTTCC AGTTCATCAT GAAGCAGAGT
    TACATCTGTG CCCTCATCGC CATGATGGCA TGGAGCATCA CCTACCACAG CTGGCTGACA
    TTTGTGCTGT TGATCTGGTC GTGCACTCTC TGGATGATTC GCAACAGGAG GAAGTACGCC
    ATGATCAGCT CTCCGTTCAT GGTTGTCTAT GCCAATCTGC TGCTGGTACT GCAGTACATA
    TGGAGCTTTG AACTTCCAGA AATCAAGAAA GTCCCTGGAT TTCTAGAAAA GAAAGAGCCT
    GGAGAGCTTG CCTCAAAGAT CCTCTTCACC ATCACATTTT GGCTGCTCCT GAGGCAGCAT
    CTCACAGAGC AGAAGGCCCT TCAGGAAAAG GAAGCACTTC TGTCTGAAGT CAAAATTGGA
    AGTCAAGAAC TTGACGAAAA GGATGAGGAA CTCCAAGATA TACAAGTGGA AGGAGAGCCC
    ATAGAGAAGG AAGAAGAGGA GGAGGAAATA AAGGAAGACA GACATGAGGT AAAGAAGGAA
    GAGGAAGAGG AAGAGGAAGA TGACGATGAC CAGGACATCA TGAAGGTTCT GGGCAATCTG
    GTGGTGGCCC TCTTCATCAA GTATTGGATC TACGTCTGCG GGGGCATGTT CTTTTTTGTC
    AGCTTTGAGG GGAAAATCGT CATGTACAAA ATCATCTACA TGGTGCTGTT CCTGTTCTGT
    GTGGCCTTGT ACCAGGTGCA CTACGAGTGG TGGAGGAAGA TCCTGAAATA CTTCTGGATG
    TCGGTGGTCA TTTACACCAT GCTGGTGCTC ATCTTCATCT ACACTTACCA GTTTGAGAAC
    TTCCCAGGCC TGTGGCAAAA CATGACCGGG TTAAAGAAAG AGAAGCTTGA GGATCTGGGC
    CTGAAGCAGT TCACTGTGGC CGAACTGTTC ACACGAATAT TCATCCCCAC CTCCTTTCTG
    CTGGTCTGCA TCCTGCACCT CCACTATTTC CATGACCGGT TCCTGGAACT CACAGACCTC
    AAGTCCATCC CCAGCAAAGA AGACAACACC ATCTACAGCC ATGCCAAGGT CAATGGTCGC
    GTGTACCTAA TAATAAATAG CATCAAGAAA AAATTACCAA TCCACCAGAA TGAGTTAGCC
    CACCCTGAAG GAAGCCTCCC AGACCTAGCC ATTATGAACA TGACGGCCAG CCTCGATAAG
    CCTGAGAGGC AGAAGTTGGC AGAGCCTGGG GAGGAGAGGA CAGAAGAATG TGCCAAGAAG
    GCAGAGAAGG GTGAGGCTGG GAAAGACAGT GATGAGTCTG AGGAGGAGGA AGGTGAGGAA
    GAGGAATCCG AGGAGGAAGA ATCCTCAGAT CTCAGGAACA AGTGGCACCT CGTGATTGAC
    CGGCTCACTG TGCTCTTTCT AAAGTTCCTG GAGTATTTCC ACAAGTTGCA GGTGTTTATG
    TGGTGGATCT TGGAGCTGCA CATCATCAAA ATCGTCTCAT CGTACATTAT CTGGGTGACT
    GTCAAAGAGG TGTCTCTGTT CAACTATGTA TTTTTGATTT CTTGGGCATT TGCTCTGCCA
    TATGCCAAAC TCCGCCGTGT GGCTTCCAGT GTCTGCACTG TCTGGACGTG TGTGATCATC
    GTCTGCAAAA TGTTGTACCA GCTGCAAACC ATTAAGCCTG AGAACTTTTC TGTCAACTGC
    TCCTTGCCCA ATGAAAATCA AACCAACATA CCCCTTAATG AGTTGAACAA GTCTCTTCTC
    TACAGTGCCC CTGTTGATCC CACTGAGTGG GTTGGCCTGA GGAAGTCCTC CCCTTTACTC
    GTCTACCTGA GGAACAACCT CCTGATGTTG GCGATTTTGG CCTTCGAAGT CACTGTTTAC
    CGACATCAGG AGTACTATCG AGGCCGAAAC AACTTGACTG CTCCGGTGTC TAAAACTATC
    TTTCATGACA TCACAAGGTT ACATCTAGAT GATGGGCTTA TCAATTGTGC CAAATATTTT
    GTAAACTACT TCTTCTACAA GTTTGGCCTA GAGACCTGTT TCCTAATGTC AGTGAACGTA
    ATTGGACAGC GAATGGACTT CTACGCCATG ATTCATGCCT GTTGGCTGAT CGGTGTCTTG
    TACCGTCGCA GAAGAAAAGC CATTGCTGAG GTCTGGCCCA AGTACTGCTG CTTCCTGGCT
    TGCATCATCA CCTTCCAGTA CTTTGTCTGC ATAGGCATCC CTCCTGCCCC CTGCCGAGAT
    TACCCATGGA GGTTCAAGGG TGCCAACTTC AATGACAACA TCATCAAGTG GCTGTACTTC
    CCAGATTTCA TCGTGCGGCC CAACCCTGTG TTTCTTGTCT ATGACTTCAT GCTGCTCCTG
    TGTGCTTCCT TACAACGACA GATTTTTGAG GACGAAAACA AGGCAGCTGT GCGCATCATG
    GCCGGGGACA ATGTCGAGAT TTGCATGAAC CTTGACGCGG CCTCATTCAG CCAGCATAAC
    CCTGTACCAG ATTTCATTCA CTGCAGATCT TACTTGGACA TGTCCAAAGT GATCATCTTC
    AGCTACCTCT TCTGGTTCGT CCTCACCATC ATCTTCATTA CCGGAACGAC CAGGATCAGC
    ATCTTTTGCA TGGGTTACCT GGTGGCCTGT TTCTATTTCC TGCTCTTTGG AGGTGATCTG
    CTCCTGAAAC CCATCAAGAG CATCCTACGC TACTGGGACT GGCTGATTGC ATACAACGTG
    TTTGTGATCA CCATGAAAAA CATACTGTCA ATAGGAGCCT GTGGATACAT CGGTGCACTG
    GTGAGAAACA GCTGCTGGCT GATCCAAGCG TTCAGCCTGG CCTGCACGGT GAAAGGCTAT
    AAAATGCCTG AAGATGACTC CAGATGCAAG CTGCCCAGTG GGGAGGCAGG GATCATCTGG
    GACAGCATCT GCTTTGCGTT TCTCCTGCTA CAGAGGAGAG TCTTCATGAG TTACTATTTC
    CTGCATGTCG TGGCTGACAT TAAAGCATCC CAGATCCTGG CATCCAGAGG GGCCGAACTT
    TTCCAGGCTA CAATTGTGAA AGCTGTAAAG GCAAGAATTG AAGAAGAGAA AAAATCCATG
    GACCAGCTGA AGAGACAGAT GGATCGCATC AAAGCCCGGC AGCAAAAGTA CAAAAAGGGT
    AAGGAGAGGA TGCTGAGCTT GACCCAGGAG TCAGGGGAAG GCCAGGACAT CCAGAAAGTC
    TCAGAAGAAG ACGATGAAAT GGTCAGGAGT GGAGATTATT ATTTGTTTGA AACGGATAGT
    GAAGAGGAGG AAGAAGAGGA ACTCAAAAAG GAGGATGAAG AACCACCACG GAAGTCTGCA
    TTCCAGAGAG CTATTGGGAA GTTTGCCTCA GCCATTTTAG CCTTGCCAAA GTCTGTCATT
    AAACTTCCCA AAACTATTCT TCAGTATTTA ATCAGAGCTG CAAAGTTCGT CTACCAAGCC
    TGGATTACCG ATCCTAAAAC AGCACTCCGA CAGAGGAGGA AAGAGAAGAA GAAAATGGCC
    AGAGAAGAGC AGAAGGGAAG ACGCAAAGGA TCTGGGGAGG GTCCAGTGGA ATGGGAAGAC
    AGAGAAGATG AGCCAATCAA AAAGAAATCT GATGGACCAG ATAATATCAT CAAGAGGATA
    TTTAATATCC TGAAGTTCAC CTGGGTCCTG TTCCTGGCCA CAGTGGACAG TTTCACTACT
    TGGCTCAACT CCATCTCAAG AGAGCATATC GACATATCCA CAGTTCTGCG GATTGAAAGA
    TGCATGCTGA CCCGAGAGAT AAAGAAGGGC AACGTTCCGA CACGGGAAAG CATCCACATG
    TACTACCAGA ACCACATGAT GAACCTTTCC AGAGAATCCG GACTGGACAC AATCGACGAG
    CACTCTGGCG CTGGTTCCAG GGCCCAGGCA GCACACAGGA TGGATAGCTT AGATTCCCAT
    GACAGTATCT CCAGCTGTTA CACTGAAGCA ACCCTGCTGA TCTCAAGGCA GTCCACCCTT
    GATGATTTAG ATGGACAAGA CCCTGTTCCT AAAACAAGCG AGCGTGCTCG GCCTAGGCTC
    CGTAAAGTGT TCAGCATGGA CATGTCCTCC TCATCAGCTG ACAGTGGCAG TGTAGCATCA
    AGCGAGCCCA CACAGTGCAC CATGCTGTAT TCACGCCAAG GAACTACGGA AACCATTGAG
    GAGGTAGAGG CTGAAGCTGA GGAGGAGGTG GTAGAGAGTC TGGAGCCTGA GCTCCATGAT
    GCCGAAGAGA AGGAATATGC AGCTGACTAT GAAGCAGGGG TAGAGGAGGT CAGCCTCACT
    CCAGATGAGG AGTTGCCTCA GTTCTCAACA GATGACGGTG ACGTGGAGGC CCCGCCCTCC
    TACAGCAAGG CTGTCAGCTT TGAGCACCTC TCGTTTGCAT CACAAGATGA CTCCGGGGGC
    AAGAACCACA TGGTGGTCAG CCCTGACGAC AGCCGCACAG ACAAGCTGGA GTCCAGTATC
    TTACCGCCCC TGACTCACGA GCTGACAGCC AGCGATCTGC TCATGAGCAA GATGTTCCGT
    GATGATGAGC TTGAAGAATC TGAGAAATTC TACGTGGACC AGCCCCGGTT CTTGCTGCTA
    TTCTATGCCA TGTATAACAC TCTGGTAGCC CGCTCGGAAA TGGTGTGCTA TTTCGTGATC
    ATCCTGAACC ACATGATCTC TGCCTCCATC ATTACTCTTC TGTTGCCCAT CCTGATCTTC
    CTCTGGGCCA TGCTGTCCGT CCCCAGGCCC AGCCGGCGCT TCTGGATGAT GGCCATCGTC
    TACACTGAGG TGGCGATTGT AGTGAAATAT TTCTTCCAGT TTGGCTTCTT TCCTTGGAAT
    AAGGACTTGG AAATTTACAA GGACAGGCCC TACTTTCCAC CAAACATCAT AGGGGTGGAA
    AAGAAAGAGG GCTATGTTCT TTACGACCTC ATTCAGCTGC TGGCTCTGTT CTTCCATCGA
    TCCATTCTGA AGTGTCATGG CCTATGGGAC GAGGATGACA TTGTTGACAG TAACACAGAC
    AAGGAAGGAT CTGATGATGA GCTGTCCCTC GGTCAGGGCA GAAGAGGCTC CTCAGAGTCA
    CTGAAGTCCA TCAACTTGGC TGCCTCTGTA GAGTCCGTCC ATGTGACCTT CCCAGAGCAG
    CCAGCAGCCA TCCGGAGAAA ACGCTCCTGC AGCAGCTCGC AGATCTCCCC ACGGTCCAGC
    TTCTCTTCAA ATAGGTCCAA AAGAGGCAGC ACAAGCACCC GAAACAGCAG CCAAAAAGGA
    AGCAGTGTTT TGAGCATTAA ACAAAAAAGC AAAAGAGAAC TTTACATGGA AAAGCTTCAA
    GAACATTTAA TCAAAGCAAA GGCCTTTACC ATAAAGAAAA CTCTACAAAT CTATGTACCC
    ATTCGGCAAT TCTTCTATGA CCTCATCCAC CCAGACTACA GTGCTGTGAC TGACGTGTAT
    GTGCTGATGT TCCTGGCCGA CACCGTGGAC TTCATCATCA TCGTCTTTGG CTTCTGGGCT
    TTTGGGAAAC ACTCGGCAGC TGCAGATATC ACCTCTTCCC TATCAGAAGA CCAGGTCCCC
    GGGCCATTCC TGGTGATGGT TCTCATTCAG TTTGGAACCA TGGTGGTGGA CCGTGCACTC
    TACCTCAGGA AGACTGTGCT TGGCAAGGTC ATTTTCCAGG TCATCCTCGT GTTTGGAATT
    CACTTCTGGA TGTTCTTCAT CCTACCCAGT GTGACTGAGA GGAAATTCAG CCAGAACCTG
    GTGGCACAGC TCTGGTACTT TGTGAAATGT GTGTACTTTG GGTTGTCTGC CTACCAAATC
    CGCTGTGGCT ACCCAACACG TGTGCTCGGA AACTTCCTCA CGAAGAGCTA CAATTATGTC
    AACCTCTTCT TGTTTCAAGG GTTTCGTCTC GTCCCATTTC TGACAGAGCT GCGGGCTGTG
    ATGGACTGGG TGTGGACAGA TACAACCTTG AGCCTCTCCA GCTGGATTTG TGTGGAGGAC
    ATTTACGCAC ACATTTTCAT CTTAAAGTGC TGGCGAGAGT CGGAAAAGAG ATACCCTCAA
    CCCAGGGGCC AGAAGAAGAA GAAAGCTGTG AAGTATGGCA TGGGCGGAAT GATCATTGTC
    CTGCTCATCT GTATCGTCTG GTTTCCTCTG CTCTTCATGT CTTTGATCAA GTCTGTCGCA
    GGGGTTATTA ACCAACCCCT GGATGTGTCC GTCACAATAA CCCTGGGAGG CTATCAGCCT
    ATCTTCACAA TGAGTGCCCA GCAAAGCCAG CTGAAAGTTA TGGATGGTAC AAAGTATAAC
    GAATTCATAA AGTCTTTCGG CCCCAACAGT GGCGCTATGC AATTTCTGGA GAATTATGAA
    AGGCAAGACA TAACAGTAGC GGAACTGGAA GGGAATTCGA ATTCTTTGTG GACCATCAGC
    CCTCCCAGTA AGCAGAAAAT GATACAGGAG CTCACCGACC CCAATAGTTG CTTCTCTGTT
    GTTTTTTCAT GGAGTATCCA GAGAAACATG ACTCTGGGTG CAAAGGCTGA AATAGCAACG
    GACAAGCTTT CTTTTCCTCT CACGGATGCG ACACGAAGTA GCATAGCCAA AATGATTGCT
    GGCAATGACA CAGAAAGTTC CAACACACCA GTGACAATAG AAAAGATCTA CCCATACTAT
    GTGAAGGCAC CCAGTGACTC AAACTCAAAG CCTATAAAAC AGCTTCTGTC AGAAAACAAT
    TTCATGAATA TCACCATCAT TTTGTTCAGA GACAATGTCA CAAAGTCCAA TAGTGAGTGG
    TGGGTTCTCA ACCTGACTGG AAGTAGGATA TTCAATCAGG GATCCCAAGC CTTGGAGCTG
    GTGGTCTTCA ATGACAAAGT CAGCCCCCCA AGTCTGGGCT TCTTGGCTGG CTATGGCATC
    ATGGGATTGT ATGCATCCGT TGTCCTTGTA ATTGGGAAGT TTGTTCGCGA ATTCTTCAGT
    GGGATTTCTC ACTCTATCAT GTTTGAAGAG CTTCCAAATG TGGACAGAAT TTTGAAGTTA
    TGCACAGATA TATTTCTCGT ACGGGAGACA GGGGAACTGG AGCTGGAGGA AGACCTCTAT
    GCCAAATTGA TATTCCTGTA CAGGTCGCCA GAAACCATGA TCAAGTGGAC TAGAGAAAAA
    ACAAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017443367.1
    CDS941..9469
    Translation

    Target ORF information:

    RefSeq Version XM_017587878.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017587878.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTCCCCAT CTGCCTGGCA 
    GTAGCATGTG CATTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCG ACGATGCAAG GACACACAGG GCGGCTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCCTTT CTGCTCCTTC ACATCATTTT CCACATCACA
    CTGGCCAGCC TGGAAGCTCA GCACCGCATC GCACCCGCTT ACAACTGCTC AACTTGGGAA
    AAGACCTTCC GGCAGATCGG CTTCGAAAGC TTGAAGGGAG CTGATGCTGG CAATGGCATC
    AGGGTGTTTG TGCCTGATAT CGGGATGTTC ATTGCGAGCC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACGGAA GAAGTAGCCC AATTTAATTC CGAGTGCGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GACTCAGAAG AAGCGCTGAT TTATGAGGAG
    GACTTAGATG GGGAAGAAGG CATGGAAGGC GAGCTGGAAG AAAGCACAAA GTTAAAAATG
    TTTCGCAGGC TCGCCTCCGT CGCTTCCAAG CTTAAGGAGT TCATCGGCAA TATGATAACC
    ACGGCGGGCA AGGTGGTGGT GACCATCCTG TTGGGCTCCT CTGGCATGAT GCTGCCATCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT CTGGGTCTGT GCACCTGGTG GTCCTGGTGC
    CGAACTTTTG ACCCATTGCT GTTTGGCTGC CTCTGTGTCC TGTTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTTCAGTTCT TCCAAGAAGC AGTCCCACCC
    AATGACTACT ATGCAAGGTT GTTTGGAATC AAATCCGTTA TTCAAACAGA CTGCTCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTACAC GCTGGCCACG CTGATCCGCA TTTGGTTGCA AGAGCCTCTT
    GTGCAGGAGG AGATGTCGAA GGAAGAGGAG GGTGCACTGG ATGGCTGCTC CAATCAGAAC
    ACAGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    CTTTTATCCA TGACCCAAGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACTGTGAAC
    GGCAACCCTG TGGACTACCA CACCATCCAC CCAAGTCTGC CCATAGAGAA TGGCCCCGCC
    AAGACGGACT TGTATACTAC TCCTCAGTAC CGGTGGGAGC CCTCAGAGGA GTCCTCAGAA
    AAGAAAGAGG AGGAAGAGGA CAAGAGAGAA GACTCAGAAG GGGAAGGGAG CCAAGAGGAA
    AAACGGAGTG TGAGAATTCA TGCCATGGTC GCCGTGTTCC AGTTCATCAT GAAGCAGAGT
    TACATCTGTG CCCTCATCGC CATGATGGCA TGGAGCATCA CCTACCACAG CTGGCTGACA
    TTTGTGCTGT TGATCTGGTC GTGCACTCTC TGGATGATTC GCAACAGGAG GAAGTACGCC
    ATGATCAGCT CTCCGTTCAT GGTTGTCTAT GCCAATCTGC TGCTGGTACT GCAGTACATA
    TGGAGCTTTG AACTTCCAGA AATCAAGAAA GTCCCTGGAT TTCTAGAAAA GAAAGAGCCT
    GGAGAGCTTG CCTCAAAGAT CCTCTTCACC ATCACATTTT GGCTGCTCCT GAGGCAGCAT
    CTCACAGAGC AGAAGGCCCT TCAGGAAAAG GAAGCACTTC TGTCTGAAGT CAAAATTGGA
    AGTCAAGAAC TTGACGAAAA GGATGAGGAA CTCCAAGATA TACAAGTGGA AGGAGAGCCC
    ATAGAGAAGG AAGAAGAGGA GGAGGAAATA AAGGAAGACA GACATGAGGT AAAGAAGGAA
    GAGGAAGAGG AAGAGGAAGA TGACGATGAC CAGGACATCA TGAAGGTTCT GGGCAATCTG
    GTGGTGGCCC TCTTCATCAA GTATTGGATC TACGTCTGCG GGGGCATGTT CTTTTTTGTC
    AGCTTTGAGG GGAAAATCGT CATGTACAAA ATCATCTACA TGGTGCTGTT CCTGTTCTGT
    GTGGCCTTGT ACCAGGTGCA CTACGAGTGG TGGAGGAAGA TCCTGAAATA CTTCTGGATG
    TCGGTGGTCA TTTACACCAT GCTGGTGCTC ATCTTCATCT ACACTTACCA GTTTGAGAAC
    TTCCCAGGCC TGTGGCAAAA CATGACCGGG TTAAAGAAAG AGAAGCTTGA GGATCTGGGC
    CTGAAGCAGT TCACTGTGGC CGAACTGTTC ACACGAATAT TCATCCCCAC CTCCTTTCTG
    CTGGTCTGCA TCCTGCACCT CCACTATTTC CATGACCGGT TCCTGGAACT CACAGACCTC
    AAGTCCATCC CCAGCAAAGA AGACAACACC ATCTACAGCC ATGCCAAGGT CAATGGTCGC
    GTGTACCTAA TAATAAATAG CATCAAGAAA AAATTACCAA TCCACCAGAA TGAGTTAGCC
    CACCCTGAAG GAAGCCTCCC AGACCTAGCC ATTATGAACA TGACGGCCAG CCTCGATAAG
    CCTGAGAGGC AGAAGTTGGC AGAGCCTGGG GAGGAGAGGA CAGAAGAATG TGCCAAGAAG
    GCAGAGAAGG GTGAGGCTGG GAAAGACAGT GATGAGTCTG AGGAGGAGGA AGGTGAGGAA
    GAGGAATCCG AGGAGGAAGA ATCCTCAGAT CTCAGGAACA AGTGGCACCT CGTGATTGAC
    CGGCTCACTG TGCTCTTTCT AAAGTTCCTG GAGTATTTCC ACAAGTTGCA GGTGTTTATG
    TGGTGGATCT TGGAGCTGCA CATCATCAAA ATCGTCTCAT CGTACATTAT CTGGGTGACT
    GTCAAAGAGG TGTCTCTGTT CAACTATGTA TTTTTGATTT CTTGGGCATT TGCTCTGCCA
    TATGCCAAAC TCCGCCGTGT GGCTTCCAGT GTCTGCACTG TCTGGACGTG TGTGATCATC
    GTCTGCAAAA TGTTGTACCA GCTGCAAACC ATTAAGCCTG AGAACTTTTC TGTCAACTGC
    TCCTTGCCCA ATGAAAATCA AACCAACATA CCCCTTAATG AGTTGAACAA GTCTCTTCTC
    TACAGTGCCC CTGTTGATCC CACTGAGTGG GTTGGCCTGA GGAAGTCCTC CCCTTTACTC
    GTCTACCTGA GGAACAACCT CCTGATGTTG GCGATTTTGG CCTTCGAAGT CACTGTTTAC
    CGACATCAGG AGTACTATCG AGGCCGAAAC AACTTGACTG CTCCGGTGTC TAAAACTATC
    TTTCATGACA TCACAAGGTT ACATCTAGAT GATGGGCTTA TCAATTGTGC CAAATATTTT
    GTAAACTACT TCTTCTACAA GTTTGGCCTA GAGACCTGTT TCCTAATGTC AGTGAACGTA
    ATTGGACAGC GAATGGACTT CTACGCCATG ATTCATGCCT GTTGGCTGAT CGGTGTCTTG
    TACCGTCGCA GAAGAAAAGC CATTGCTGAG GTCTGGCCCA AGTACTGCTG CTTCCTGGCT
    TGCATCATCA CCTTCCAGTA CTTTGTCTGC ATAGGCATCC CTCCTGCCCC CTGCCGAGAT
    TACCCATGGA GGTTCAAGGG TGCCAACTTC AATGACAACA TCATCAAGTG GCTGTACTTC
    CCAGATTTCA TCGTGCGGCC CAACCCTGTG TTTCTTGTCT ATGACTTCAT GCTGCTCCTG
    TGTGCTTCCT TACAACGACA GATTTTTGAG GACGAAAACA AGGCAGCTGT GCGCATCATG
    GCCGGGGACA ATGTCGAGAT TTGCATGAAC CTTGACGCGG CCTCATTCAG CCAGCATAAC
    CCTGTACCAG ATTTCATTCA CTGCAGATCT TACTTGGACA TGTCCAAAGT GATCATCTTC
    AGCTACCTCT TCTGGTTCGT CCTCACCATC ATCTTCATTA CCGGAACGAC CAGGATCAGC
    ATCTTTTGCA TGGGTTACCT GGTGGCCTGT TTCTATTTCC TGCTCTTTGG AGGTGATCTG
    CTCCTGAAAC CCATCAAGAG CATCCTACGC TACTGGGACT GGCTGATTGC ATACAACGTG
    TTTGTGATCA CCATGAAAAA CATACTGTCA ATAGGAGCCT GTGGATACAT CGGTGCACTG
    GTGAGAAACA GCTGCTGGCT GATCCAAGCG TTCAGCCTGG CCTGCACGGT GAAAGGCTAT
    AAAATGCCTG AAGATGACTC CAGATGCAAG CTGCCCAGTG GGGAGGCAGG GATCATCTGG
    GACAGCATCT GCTTTGCGTT TCTCCTGCTA CAGAGGAGAG TCTTCATGAG TTACTATTTC
    CTGCATGTCG TGGCTGACAT TAAAGCATCC CAGATCCTGG CATCCAGAGG GGCCGAACTT
    TTCCAGGCTA CAATTGTGAA AGCTGTAAAG GCAAGAATTG AAGAAGAGAA AAAATCCATG
    GACCAGCTGA AGAGACAGAT GGATCGCATC AAAGCCCGGC AGCAAAAGTA CAAAAAGGGT
    AAGGAGAGGA TGCTGAGCTT GACCCAGGAG TCAGGGGAAG GCCAGGACAT CCAGAAAGTC
    TCAGAAGAAG ACGATGAAAT GGTCAGGAGT GGAGATTATT ATTTGTTTGA AACGGATAGT
    GAAGAGGAGG AAGAAGAGGA ACTCAAAAAG GAGGATGAAG AACCACCACG GAAGTCTGCA
    TTCCAGAGAG CTATTGGGAA GTTTGCCTCA GCCATTTTAG CCTTGCCAAA GTCTGTCATT
    AAACTTCCCA AAACTATTCT TCAGTATTTA ATCAGAGCTG CAAAGTTCGT CTACCAAGCC
    TGGATTACCG ATCCTAAAAC AGCACTCCGA CAGAGGAGGA AAGAGAAGAA GAAAATGGCC
    AGAGAAGAGC AGAAGGGAAG ACGCAAAGGA TCTGGGGAGG GTCCAGTGGA ATGGGAAGAC
    AGAGAAGATG AGCCAATCAA AAAGAAATCT GATGGACCAG ATAATATCAT CAAGAGGATA
    TTTAATATCC TGAAGTTCAC CTGGGTCCTG TTCCTGGCCA CAGTGGACAG TTTCACTACT
    TGGCTCAACT CCATCTCAAG AGAGCATATC GACATATCCA CAGTTCTGCG GATTGAAAGA
    TGCATGCTGA CCCGAGAGAT AAAGAAGGGC AACGTTCCGA CACGGGAAAG CATCCACATG
    TACTACCAGA ACCACATGAT GAACCTTTCC AGAGAATCCG GACTGGACAC AATCGACGAG
    CACTCTGGCG CTGGTTCCAG GGCCCAGGCA GCACACAGGA TGGATAGCTT AGATTCCCAT
    GACAGTATCT CCAGCTGTTA CACTGAAGCA ACCCTGCTGA TCTCAAGGCA GTCCACCCTT
    GATGATTTAG ATGGACAAGA CCCTGTTCCT AAAACAAGCG AGCGTGCTCG GCCTAGGCTC
    CGTAAAGTGT TCAGCATGGA CATGTCCTCC TCATCAGCTG ACAGTGGCAG TGTAGCATCA
    AGCGAGCCCA CACAGTGCAC CATGCTGTAT TCACGCCAAG GAACTACGGA AACCATTGAG
    GAGGTAGAGG CTGAAGCTGA GGAGGAGGTG GTAGAGAGTC TGGAGCCTGA GCTCCATGAT
    GCCGAAGAGA AGGAATATGC AGCTGACTAT GAAGCAGGGG TAGAGGAGGT CAGCCTCACT
    CCAGATGAGG AGTTGCCTCA GTTCTCAACA GATGACGGTG ACGTGGAGGC CCCGCCCTCC
    TACAGCAAGG CTGTCAGCTT TGAGCACCTC TCGTTTGCAT CACAAGATGA CTCCGGGGGC
    AAGAACCACA TGGTGGTCAG CCCTGACGAC AGCCGCACAG ACAAGCTGGA GTCCAGTATC
    TTACCGCCCC TGACTCACGA GCTGACAGCC AGCGATCTGC TCATGAGCAA GATGTTCCGT
    GATGATGAGC TTGAAGAATC TGAGAAATTC TACGTGGACC AGCCCCGGTT CTTGCTGCTA
    TTCTATGCCA TGTATAACAC TCTGGTAGCC CGCTCGGAAA TGGTGTGCTA TTTCGTGATC
    ATCCTGAACC ACATGATCTC TGCCTCCATC ATTACTCTTC TGTTGCCCAT CCTGATCTTC
    CTCTGGGCCA TGCTGTCCGT CCCCAGGCCC AGCCGGCGCT TCTGGATGAT GGCCATCGTC
    TACACTGAGG TGGCGATTGT AGTGAAATAT TTCTTCCAGT TTGGCTTCTT TCCTTGGAAT
    AAGGACTTGG AAATTTACAA GGACAGGCCC TACTTTCCAC CAAACATCAT AGGGGTGGAA
    AAGAAAGAGG GCTATGTTCT TTACGACCTC ATTCAGCTGC TGGCTCTGTT CTTCCATCGA
    TCCATTCTGA AGTGTCATGG CCTATGGGAC GAGGATGACA TTGTTGACAG TAACACAGAC
    AAGGAAGGAT CTGATGATGA GCTGTCCCTC GGTCAGGGCA GAAGAGGCTC CTCAGAGTCA
    CTGAAGTCCA TCAACTTGGC TGCCTCTGTA GAGTCCGTCC ATGTGACCTT CCCAGAGCAG
    CCAGCAGCCA TCCGGAGAAA ACGCTCCTGC AGCAGCTCGC AGATCTCCCC ACGGTCCAGC
    TTCTCTTCAA ATAGGTCCAA AAGAGGCAGC ACAAGCACCC GAAACAGCAG CCAAAAAGGA
    AGCAGTGTTT TGAGCATTAA ACAAAAAAGC AAAAGAGAAC TTTACATGGA AAAGCTTCAA
    GAACATTTAA TCAAAGCAAA GGCCTTTACC ATAAAGAAAA CTCTACAAAT CTATGTACCC
    ATTCGGCAAT TCTTCTATGA CCTCATCCAC CCAGACTACA GTGCTGTGAC TGACGTGTAT
    GTGCTGATGT TCCTGGCCGA CACCGTGGAC TTCATCATCA TCGTCTTTGG CTTCTGGGCT
    TTTGGGAAAC ACTCGGCAGC TGCAGATATC ACCTCTTCCC TATCAGAAGA CCAGGTCCCC
    GGGCCATTCC TGGTGATGGT TCTCATTCAG TTTGGAACCA TGGTGGTGGA CCGTGCACTC
    TACCTCAGGA AGACTGTGCT TGGCAAGGTC ATTTTCCAGG TCATCCTCGT GTTTGGAATT
    CACTTCTGGA TGTTCTTCAT CCTACCCAGT GTGACTGAGA GGAAATTCAG CCAGAACCTG
    GTGGCACAGC TCTGGTACTT TGTGAAATGT GTGTACTTTG GGTTGTCTGC CTACCAAATC
    CGCTGTGGCT ACCCAACACG TGTGCTCGGA AACTTCCTCA CGAAGAGCTA CAATTATGTC
    AACCTCTTCT TGTTTCAAGG GTTTCGTCTC GTCCCATTTC TGACAGAGCT GCGGGCTGTG
    ATGGACTGGG TGTGGACAGA TACAACCTTG AGCCTCTCCA GCTGGATTTG TGTGGAGGAC
    ATTTACGCAC ACATTTTCAT CTTAAAGTGC TGGCGAGAGT CGGAAAAGAG ATACCCTCAA
    CCCAGGGGCC AGAAGAAGAA GAAAGCTGTG AAGTATGGCA TGGGCGGAAT GATCATTGTC
    CTGCTCATCT GTATCGTCTG GTTTCCTCTG CTCTTCATGT CTTTGATCAA GTCTGTCGCA
    GGGGTTATTA ACCAACCCCT GGATGTGTCC GTCACAATAA CCCTGGGAGG CTATCAGCCT
    ATCTTCACAA TGAGTGCCCA GCAAAGCCAG CTGAAAGTTA TGGATGGTAC AAAGTATAAC
    GAATTCATAA AGTCTTTCGG CCCCAACAGT GGCGCTATGC AATTTCTGGA GAATTATGAA
    AGGCAAGACA TAACAGTAGC GGAACTGGAA GGGAATTCGA ATTCTTTGTG GACCATCAGC
    CCTCCCAGTA AGCAGAAAAT GATACAGGAG CTCACCGACC CCAATAGTTG CTTCTCTGTT
    GTTTTTTCAT GGAGTATCCA GAGAAACATG ACTCTGGGTG CAAAGGCTGA AATAGCAACG
    GACAAGCTTT CTTTTCCTCT CACGGATGCG ACACGAAGTA GCATAGCCAA AATGATTGCT
    GGCAATGACA CAGAAAGTTC CAACACACCA GTGACAATAG AAAAGATCTA CCCATACTAT
    GTGAAGGCAC CCAGTGACTC AAACTCAAAG CCTATAAAAC AGCTTCTGTC AGAAAACAAT
    TTCATGAATA TCACCATCAT TTTGTTCAGA GACAATGTCA CAAAGTCCAA TAGTGAGTGG
    TGGGTTCTCA ACCTGACTGG AAGTAGGATA TTCAATCAGG GATCCCAAGC CTTGGAGCTG
    GTGGTCTTCA ATGACAAAGT CAGCCCCCCA AGTCTGGGCT TCTTGGCTGG CTATGGCATC
    ATGGGATTGT ATGCATCCGT TGTCCTTGTA ATTGGGAAGT TTGTTCGCGA ATTCTTCAGT
    GGGATTTCTC ACTCTATCAT GTTTGAAGAG CTTCCAAATG TGGACAGAAT TTTGAAGTTA
    TGCACAGATA TATTTCTCGT ACGGGAGACA GGGGAACTGG AGCTGGAGGA AGACCTCTAT
    GCCAAATTGA TATTCCTGTA CAGGTCGCCA GAAACCATGA TCAAGTGGAC TAGAGAAAAA
    ACAAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa54719
    Clone ID Related Accession (Same CDS sequence) XM_017587878.1 , XM_017601117.1
    Accession Version XM_017601117.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8529bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product piezo-type mechanosensitive ion channel component 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005117.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTCCCCAT CTGCCTGGCA 
    GTAGCATGTG CATTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCG ACGATGCAAG GACACACAGG GCGGCTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCCTTT CTGCTCCTTC ACATCATTTT CCACATCACA
    CTGGCCAGCC TGGAAGCTCA GCACCGCATC GCACCCGCTT ACAACTGCTC AACTTGGGAA
    AAGACCTTCC GGCAGATCGG CTTCGAAAGC TTGAAGGGAG CTGATGCTGG CAATGGCATC
    AGGGTGTTTG TGCCTGATAT CGGGATGTTC ATTGCGAGCC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACGGAA GAAGTAGCCC AATTTAATTC CGAGTGCGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GACTCAGAAG AAGCGCTGAT TTATGAGGAG
    GACTTAGATG GGGAAGAAGG CATGGAAGGC GAGCTGGAAG AAAGCACAAA GTTAAAAATG
    TTTCGCAGGC TCGCCTCCGT CGCTTCCAAG CTTAAGGAGT TCATCGGCAA TATGATAACC
    ACGGCGGGCA AGGTGGTGGT GACCATCCTG TTGGGCTCCT CTGGCATGAT GCTGCCATCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT CTGGGTCTGT GCACCTGGTG GTCCTGGTGC
    CGAACTTTTG ACCCATTGCT GTTTGGCTGC CTCTGTGTCC TGTTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTTCAGTTCT TCCAAGAAGC AGTCCCACCC
    AATGACTACT ATGCAAGGTT GTTTGGAATC AAATCCGTTA TTCAAACAGA CTGCTCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTACAC GCTGGCCACG CTGATCCGCA TTTGGTTGCA AGAGCCTCTT
    GTGCAGGAGG AGATGTCGAA GGAAGAGGAG GGTGCACTGG ATGGCTGCTC CAATCAGAAC
    ACAGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    CTTTTATCCA TGACCCAAGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACTGTGAAC
    GGCAACCCTG TGGACTACCA CACCATCCAC CCAAGTCTGC CCATAGAGAA TGGCCCCGCC
    AAGACGGACT TGTATACTAC TCCTCAGTAC CGGTGGGAGC CCTCAGAGGA GTCCTCAGAA
    AAGAAAGAGG AGGAAGAGGA CAAGAGAGAA GACTCAGAAG GGGAAGGGAG CCAAGAGGAA
    AAACGGAGTG TGAGAATTCA TGCCATGGTC GCCGTGTTCC AGTTCATCAT GAAGCAGAGT
    TACATCTGTG CCCTCATCGC CATGATGGCA TGGAGCATCA CCTACCACAG CTGGCTGACA
    TTTGTGCTGT TGATCTGGTC GTGCACTCTC TGGATGATTC GCAACAGGAG GAAGTACGCC
    ATGATCAGCT CTCCGTTCAT GGTTGTCTAT GCCAATCTGC TGCTGGTACT GCAGTACATA
    TGGAGCTTTG AACTTCCAGA AATCAAGAAA GTCCCTGGAT TTCTAGAAAA GAAAGAGCCT
    GGAGAGCTTG CCTCAAAGAT CCTCTTCACC ATCACATTTT GGCTGCTCCT GAGGCAGCAT
    CTCACAGAGC AGAAGGCCCT TCAGGAAAAG GAAGCACTTC TGTCTGAAGT CAAAATTGGA
    AGTCAAGAAC TTGACGAAAA GGATGAGGAA CTCCAAGATA TACAAGTGGA AGGAGAGCCC
    ATAGAGAAGG AAGAAGAGGA GGAGGAAATA AAGGAAGACA GACATGAGGT AAAGAAGGAA
    GAGGAAGAGG AAGAGGAAGA TGACGATGAC CAGGACATCA TGAAGGTTCT GGGCAATCTG
    GTGGTGGCCC TCTTCATCAA GTATTGGATC TACGTCTGCG GGGGCATGTT CTTTTTTGTC
    AGCTTTGAGG GGAAAATCGT CATGTACAAA ATCATCTACA TGGTGCTGTT CCTGTTCTGT
    GTGGCCTTGT ACCAGGTGCA CTACGAGTGG TGGAGGAAGA TCCTGAAATA CTTCTGGATG
    TCGGTGGTCA TTTACACCAT GCTGGTGCTC ATCTTCATCT ACACTTACCA GTTTGAGAAC
    TTCCCAGGCC TGTGGCAAAA CATGACCGGG TTAAAGAAAG AGAAGCTTGA GGATCTGGGC
    CTGAAGCAGT TCACTGTGGC CGAACTGTTC ACACGAATAT TCATCCCCAC CTCCTTTCTG
    CTGGTCTGCA TCCTGCACCT CCACTATTTC CATGACCGGT TCCTGGAACT CACAGACCTC
    AAGTCCATCC CCAGCAAAGA AGACAACACC ATCTACAGCC ATGCCAAGGT CAATGGTCGC
    GTGTACCTAA TAATAAATAG CATCAAGAAA AAATTACCAA TCCACCAGAA TGAGTTAGCC
    CACCCTGAAG GAAGCCTCCC AGACCTAGCC ATTATGAACA TGACGGCCAG CCTCGATAAG
    CCTGAGAGGC AGAAGTTGGC AGAGCCTGGG GAGGAGAGGA CAGAAGAATG TGCCAAGAAG
    GCAGAGAAGG GTGAGGCTGG GAAAGACAGT GATGAGTCTG AGGAGGAGGA AGGTGAGGAA
    GAGGAATCCG AGGAGGAAGA ATCCTCAGAT CTCAGGAACA AGTGGCACCT CGTGATTGAC
    CGGCTCACTG TGCTCTTTCT AAAGTTCCTG GAGTATTTCC ACAAGTTGCA GGTGTTTATG
    TGGTGGATCT TGGAGCTGCA CATCATCAAA ATCGTCTCAT CGTACATTAT CTGGGTGACT
    GTCAAAGAGG TGTCTCTGTT CAACTATGTA TTTTTGATTT CTTGGGCATT TGCTCTGCCA
    TATGCCAAAC TCCGCCGTGT GGCTTCCAGT GTCTGCACTG TCTGGACGTG TGTGATCATC
    GTCTGCAAAA TGTTGTACCA GCTGCAAACC ATTAAGCCTG AGAACTTTTC TGTCAACTGC
    TCCTTGCCCA ATGAAAATCA AACCAACATA CCCCTTAATG AGTTGAACAA GTCTCTTCTC
    TACAGTGCCC CTGTTGATCC CACTGAGTGG GTTGGCCTGA GGAAGTCCTC CCCTTTACTC
    GTCTACCTGA GGAACAACCT CCTGATGTTG GCGATTTTGG CCTTCGAAGT CACTGTTTAC
    CGACATCAGG AGTACTATCG AGGCCGAAAC AACTTGACTG CTCCGGTGTC TAAAACTATC
    TTTCATGACA TCACAAGGTT ACATCTAGAT GATGGGCTTA TCAATTGTGC CAAATATTTT
    GTAAACTACT TCTTCTACAA GTTTGGCCTA GAGACCTGTT TCCTAATGTC AGTGAACGTA
    ATTGGACAGC GAATGGACTT CTACGCCATG ATTCATGCCT GTTGGCTGAT CGGTGTCTTG
    TACCGTCGCA GAAGAAAAGC CATTGCTGAG GTCTGGCCCA AGTACTGCTG CTTCCTGGCT
    TGCATCATCA CCTTCCAGTA CTTTGTCTGC ATAGGCATCC CTCCTGCCCC CTGCCGAGAT
    TACCCATGGA GGTTCAAGGG TGCCAACTTC AATGACAACA TCATCAAGTG GCTGTACTTC
    CCAGATTTCA TCGTGCGGCC CAACCCTGTG TTTCTTGTCT ATGACTTCAT GCTGCTCCTG
    TGTGCTTCCT TACAACGACA GATTTTTGAG GACGAAAACA AGGCAGCTGT GCGCATCATG
    GCCGGGGACA ATGTCGAGAT TTGCATGAAC CTTGACGCGG CCTCATTCAG CCAGCATAAC
    CCTGTACCAG ATTTCATTCA CTGCAGATCT TACTTGGACA TGTCCAAAGT GATCATCTTC
    AGCTACCTCT TCTGGTTCGT CCTCACCATC ATCTTCATTA CCGGAACGAC CAGGATCAGC
    ATCTTTTGCA TGGGTTACCT GGTGGCCTGT TTCTATTTCC TGCTCTTTGG AGGTGATCTG
    CTCCTGAAAC CCATCAAGAG CATCCTACGC TACTGGGACT GGCTGATTGC ATACAACGTG
    TTTGTGATCA CCATGAAAAA CATACTGTCA ATAGGAGCCT GTGGATACAT CGGTGCACTG
    GTGAGAAACA GCTGCTGGCT GATCCAAGCG TTCAGCCTGG CCTGCACGGT GAAAGGCTAT
    AAAATGCCTG AAGATGACTC CAGATGCAAG CTGCCCAGTG GGGAGGCAGG GATCATCTGG
    GACAGCATCT GCTTTGCGTT TCTCCTGCTA CAGAGGAGAG TCTTCATGAG TTACTATTTC
    CTGCATGTCG TGGCTGACAT TAAAGCATCC CAGATCCTGG CATCCAGAGG GGCCGAACTT
    TTCCAGGCTA CAATTGTGAA AGCTGTAAAG GCAAGAATTG AAGAAGAGAA AAAATCCATG
    GACCAGCTGA AGAGACAGAT GGATCGCATC AAAGCCCGGC AGCAAAAGTA CAAAAAGGGT
    AAGGAGAGGA TGCTGAGCTT GACCCAGGAG TCAGGGGAAG GCCAGGACAT CCAGAAAGTC
    TCAGAAGAAG ACGATGAAAT GGTCAGGAGT GGAGATTATT ATTTGTTTGA AACGGATAGT
    GAAGAGGAGG AAGAAGAGGA ACTCAAAAAG GAGGATGAAG AACCACCACG GAAGTCTGCA
    TTCCAGAGAG CTATTGGGAA GTTTGCCTCA GCCATTTTAG CCTTGCCAAA GTCTGTCATT
    AAACTTCCCA AAACTATTCT TCAGTATTTA ATCAGAGCTG CAAAGTTCGT CTACCAAGCC
    TGGATTACCG ATCCTAAAAC AGCACTCCGA CAGAGGAGGA AAGAGAAGAA GAAAATGGCC
    AGAGAAGAGC AGAAGGGAAG ACGCAAAGGA TCTGGGGAGG GTCCAGTGGA ATGGGAAGAC
    AGAGAAGATG AGCCAATCAA AAAGAAATCT GATGGACCAG ATAATATCAT CAAGAGGATA
    TTTAATATCC TGAAGTTCAC CTGGGTCCTG TTCCTGGCCA CAGTGGACAG TTTCACTACT
    TGGCTCAACT CCATCTCAAG AGAGCATATC GACATATCCA CAGTTCTGCG GATTGAAAGA
    TGCATGCTGA CCCGAGAGAT AAAGAAGGGC AACGTTCCGA CACGGGAAAG CATCCACATG
    TACTACCAGA ACCACATGAT GAACCTTTCC AGAGAATCCG GACTGGACAC AATCGACGAG
    CACTCTGGCG CTGGTTCCAG GGCCCAGGCA GCACACAGGA TGGATAGCTT AGATTCCCAT
    GACAGTATCT CCAGCTGTTA CACTGAAGCA ACCCTGCTGA TCTCAAGGCA GTCCACCCTT
    GATGATTTAG ATGGACAAGA CCCTGTTCCT AAAACAAGCG AGCGTGCTCG GCCTAGGCTC
    CGTAAAGTGT TCAGCATGGA CATGTCCTCC TCATCAGCTG ACAGTGGCAG TGTAGCATCA
    AGCGAGCCCA CACAGTGCAC CATGCTGTAT TCACGCCAAG GAACTACGGA AACCATTGAG
    GAGGTAGAGG CTGAAGCTGA GGAGGAGGTG GTAGAGAGTC TGGAGCCTGA GCTCCATGAT
    GCCGAAGAGA AGGAATATGC AGCTGACTAT GAAGCAGGGG TAGAGGAGGT CAGCCTCACT
    CCAGATGAGG AGTTGCCTCA GTTCTCAACA GATGACGGTG ACGTGGAGGC CCCGCCCTCC
    TACAGCAAGG CTGTCAGCTT TGAGCACCTC TCGTTTGCAT CACAAGATGA CTCCGGGGGC
    AAGAACCACA TGGTGGTCAG CCCTGACGAC AGCCGCACAG ACAAGCTGGA GTCCAGTATC
    TTACCGCCCC TGACTCACGA GCTGACAGCC AGCGATCTGC TCATGAGCAA GATGTTCCGT
    GATGATGAGC TTGAAGAATC TGAGAAATTC TACGTGGACC AGCCCCGGTT CTTGCTGCTA
    TTCTATGCCA TGTATAACAC TCTGGTAGCC CGCTCGGAAA TGGTGTGCTA TTTCGTGATC
    ATCCTGAACC ACATGATCTC TGCCTCCATC ATTACTCTTC TGTTGCCCAT CCTGATCTTC
    CTCTGGGCCA TGCTGTCCGT CCCCAGGCCC AGCCGGCGCT TCTGGATGAT GGCCATCGTC
    TACACTGAGG TGGCGATTGT AGTGAAATAT TTCTTCCAGT TTGGCTTCTT TCCTTGGAAT
    AAGGACTTGG AAATTTACAA GGACAGGCCC TACTTTCCAC CAAACATCAT AGGGGTGGAA
    AAGAAAGAGG GCTATGTTCT TTACGACCTC ATTCAGCTGC TGGCTCTGTT CTTCCATCGA
    TCCATTCTGA AGTGTCATGG CCTATGGGAC GAGGATGACA TTGTTGACAG TAACACAGAC
    AAGGAAGGAT CTGATGATGA GCTGTCCCTC GGTCAGGGCA GAAGAGGCTC CTCAGAGTCA
    CTGAAGTCCA TCAACTTGGC TGCCTCTGTA GAGTCCGTCC ATGTGACCTT CCCAGAGCAG
    CCAGCAGCCA TCCGGAGAAA ACGCTCCTGC AGCAGCTCGC AGATCTCCCC ACGGTCCAGC
    TTCTCTTCAA ATAGGTCCAA AAGAGGCAGC ACAAGCACCC GAAACAGCAG CCAAAAAGGA
    AGCAGTGTTT TGAGCATTAA ACAAAAAAGC AAAAGAGAAC TTTACATGGA AAAGCTTCAA
    GAACATTTAA TCAAAGCAAA GGCCTTTACC ATAAAGAAAA CTCTACAAAT CTATGTACCC
    ATTCGGCAAT TCTTCTATGA CCTCATCCAC CCAGACTACA GTGCTGTGAC TGACGTGTAT
    GTGCTGATGT TCCTGGCCGA CACCGTGGAC TTCATCATCA TCGTCTTTGG CTTCTGGGCT
    TTTGGGAAAC ACTCGGCAGC TGCAGATATC ACCTCTTCCC TATCAGAAGA CCAGGTCCCC
    GGGCCATTCC TGGTGATGGT TCTCATTCAG TTTGGAACCA TGGTGGTGGA CCGTGCACTC
    TACCTCAGGA AGACTGTGCT TGGCAAGGTC ATTTTCCAGG TCATCCTCGT GTTTGGAATT
    CACTTCTGGA TGTTCTTCAT CCTACCCAGT GTGACTGAGA GGAAATTCAG CCAGAACCTG
    GTGGCACAGC TCTGGTACTT TGTGAAATGT GTGTACTTTG GGTTGTCTGC CTACCAAATC
    CGCTGTGGCT ACCCAACACG TGTGCTCGGA AACTTCCTCA CGAAGAGCTA CAATTATGTC
    AACCTCTTCT TGTTTCAAGG GTTTCGTCTC GTCCCATTTC TGACAGAGCT GCGGGCTGTG
    ATGGACTGGG TGTGGACAGA TACAACCTTG AGCCTCTCCA GCTGGATTTG TGTGGAGGAC
    ATTTACGCAC ACATTTTCAT CTTAAAGTGC TGGCGAGAGT CGGAAAAGAG ATACCCTCAA
    CCCAGGGGCC AGAAGAAGAA GAAAGCTGTG AAGTATGGCA TGGGCGGAAT GATCATTGTC
    CTGCTCATCT GTATCGTCTG GTTTCCTCTG CTCTTCATGT CTTTGATCAA GTCTGTCGCA
    GGGGTTATTA ACCAACCCCT GGATGTGTCC GTCACAATAA CCCTGGGAGG CTATCAGCCT
    ATCTTCACAA TGAGTGCCCA GCAAAGCCAG CTGAAAGTTA TGGATGGTAC AAAGTATAAC
    GAATTCATAA AGTCTTTCGG CCCCAACAGT GGCGCTATGC AATTTCTGGA GAATTATGAA
    AGGCAAGACA TAACAGTAGC GGAACTGGAA GGGAATTCGA ATTCTTTGTG GACCATCAGC
    CCTCCCAGTA AGCAGAAAAT GATACAGGAG CTCACCGACC CCAATAGTTG CTTCTCTGTT
    GTTTTTTCAT GGAGTATCCA GAGAAACATG ACTCTGGGTG CAAAGGCTGA AATAGCAACG
    GACAAGCTTT CTTTTCCTCT CACGGATGCG ACACGAAGTA GCATAGCCAA AATGATTGCT
    GGCAATGACA CAGAAAGTTC CAACACACCA GTGACAATAG AAAAGATCTA CCCATACTAT
    GTGAAGGCAC CCAGTGACTC AAACTCAAAG CCTATAAAAC AGCTTCTGTC AGAAAACAAT
    TTCATGAATA TCACCATCAT TTTGTTCAGA GACAATGTCA CAAAGTCCAA TAGTGAGTGG
    TGGGTTCTCA ACCTGACTGG AAGTAGGATA TTCAATCAGG GATCCCAAGC CTTGGAGCTG
    GTGGTCTTCA ATGACAAAGT CAGCCCCCCA AGTCTGGGCT TCTTGGCTGG CTATGGCATC
    ATGGGATTGT ATGCATCCGT TGTCCTTGTA ATTGGGAAGT TTGTTCGCGA ATTCTTCAGT
    GGGATTTCTC ACTCTATCAT GTTTGAAGAG CTTCCAAATG TGGACAGAAT TTTGAAGTTA
    TGCACAGATA TATTTCTCGT ACGGGAGACA GGGGAACTGG AGCTGGAGGA AGACCTCTAT
    GCCAAATTGA TATTCCTGTA CAGGTCGCCA GAAACCATGA TCAAGTGGAC TAGAGAAAAA
    ACAAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017456606.1
    CDS941..9469
    Translation

    Target ORF information:

    RefSeq Version XM_017601117.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017601117.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTCCCCAT CTGCCTGGCA 
    GTAGCATGTG CATTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCG ACGATGCAAG GACACACAGG GCGGCTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCCTTT CTGCTCCTTC ACATCATTTT CCACATCACA
    CTGGCCAGCC TGGAAGCTCA GCACCGCATC GCACCCGCTT ACAACTGCTC AACTTGGGAA
    AAGACCTTCC GGCAGATCGG CTTCGAAAGC TTGAAGGGAG CTGATGCTGG CAATGGCATC
    AGGGTGTTTG TGCCTGATAT CGGGATGTTC ATTGCGAGCC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACGGAA GAAGTAGCCC AATTTAATTC CGAGTGCGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GACTCAGAAG AAGCGCTGAT TTATGAGGAG
    GACTTAGATG GGGAAGAAGG CATGGAAGGC GAGCTGGAAG AAAGCACAAA GTTAAAAATG
    TTTCGCAGGC TCGCCTCCGT CGCTTCCAAG CTTAAGGAGT TCATCGGCAA TATGATAACC
    ACGGCGGGCA AGGTGGTGGT GACCATCCTG TTGGGCTCCT CTGGCATGAT GCTGCCATCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT CTGGGTCTGT GCACCTGGTG GTCCTGGTGC
    CGAACTTTTG ACCCATTGCT GTTTGGCTGC CTCTGTGTCC TGTTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTTCAGTTCT TCCAAGAAGC AGTCCCACCC
    AATGACTACT ATGCAAGGTT GTTTGGAATC AAATCCGTTA TTCAAACAGA CTGCTCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTACAC GCTGGCCACG CTGATCCGCA TTTGGTTGCA AGAGCCTCTT
    GTGCAGGAGG AGATGTCGAA GGAAGAGGAG GGTGCACTGG ATGGCTGCTC CAATCAGAAC
    ACAGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    CTTTTATCCA TGACCCAAGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACTGTGAAC
    GGCAACCCTG TGGACTACCA CACCATCCAC CCAAGTCTGC CCATAGAGAA TGGCCCCGCC
    AAGACGGACT TGTATACTAC TCCTCAGTAC CGGTGGGAGC CCTCAGAGGA GTCCTCAGAA
    AAGAAAGAGG AGGAAGAGGA CAAGAGAGAA GACTCAGAAG GGGAAGGGAG CCAAGAGGAA
    AAACGGAGTG TGAGAATTCA TGCCATGGTC GCCGTGTTCC AGTTCATCAT GAAGCAGAGT
    TACATCTGTG CCCTCATCGC CATGATGGCA TGGAGCATCA CCTACCACAG CTGGCTGACA
    TTTGTGCTGT TGATCTGGTC GTGCACTCTC TGGATGATTC GCAACAGGAG GAAGTACGCC
    ATGATCAGCT CTCCGTTCAT GGTTGTCTAT GCCAATCTGC TGCTGGTACT GCAGTACATA
    TGGAGCTTTG AACTTCCAGA AATCAAGAAA GTCCCTGGAT TTCTAGAAAA GAAAGAGCCT
    GGAGAGCTTG CCTCAAAGAT CCTCTTCACC ATCACATTTT GGCTGCTCCT GAGGCAGCAT
    CTCACAGAGC AGAAGGCCCT TCAGGAAAAG GAAGCACTTC TGTCTGAAGT CAAAATTGGA
    AGTCAAGAAC TTGACGAAAA GGATGAGGAA CTCCAAGATA TACAAGTGGA AGGAGAGCCC
    ATAGAGAAGG AAGAAGAGGA GGAGGAAATA AAGGAAGACA GACATGAGGT AAAGAAGGAA
    GAGGAAGAGG AAGAGGAAGA TGACGATGAC CAGGACATCA TGAAGGTTCT GGGCAATCTG
    GTGGTGGCCC TCTTCATCAA GTATTGGATC TACGTCTGCG GGGGCATGTT CTTTTTTGTC
    AGCTTTGAGG GGAAAATCGT CATGTACAAA ATCATCTACA TGGTGCTGTT CCTGTTCTGT
    GTGGCCTTGT ACCAGGTGCA CTACGAGTGG TGGAGGAAGA TCCTGAAATA CTTCTGGATG
    TCGGTGGTCA TTTACACCAT GCTGGTGCTC ATCTTCATCT ACACTTACCA GTTTGAGAAC
    TTCCCAGGCC TGTGGCAAAA CATGACCGGG TTAAAGAAAG AGAAGCTTGA GGATCTGGGC
    CTGAAGCAGT TCACTGTGGC CGAACTGTTC ACACGAATAT TCATCCCCAC CTCCTTTCTG
    CTGGTCTGCA TCCTGCACCT CCACTATTTC CATGACCGGT TCCTGGAACT CACAGACCTC
    AAGTCCATCC CCAGCAAAGA AGACAACACC ATCTACAGCC ATGCCAAGGT CAATGGTCGC
    GTGTACCTAA TAATAAATAG CATCAAGAAA AAATTACCAA TCCACCAGAA TGAGTTAGCC
    CACCCTGAAG GAAGCCTCCC AGACCTAGCC ATTATGAACA TGACGGCCAG CCTCGATAAG
    CCTGAGAGGC AGAAGTTGGC AGAGCCTGGG GAGGAGAGGA CAGAAGAATG TGCCAAGAAG
    GCAGAGAAGG GTGAGGCTGG GAAAGACAGT GATGAGTCTG AGGAGGAGGA AGGTGAGGAA
    GAGGAATCCG AGGAGGAAGA ATCCTCAGAT CTCAGGAACA AGTGGCACCT CGTGATTGAC
    CGGCTCACTG TGCTCTTTCT AAAGTTCCTG GAGTATTTCC ACAAGTTGCA GGTGTTTATG
    TGGTGGATCT TGGAGCTGCA CATCATCAAA ATCGTCTCAT CGTACATTAT CTGGGTGACT
    GTCAAAGAGG TGTCTCTGTT CAACTATGTA TTTTTGATTT CTTGGGCATT TGCTCTGCCA
    TATGCCAAAC TCCGCCGTGT GGCTTCCAGT GTCTGCACTG TCTGGACGTG TGTGATCATC
    GTCTGCAAAA TGTTGTACCA GCTGCAAACC ATTAAGCCTG AGAACTTTTC TGTCAACTGC
    TCCTTGCCCA ATGAAAATCA AACCAACATA CCCCTTAATG AGTTGAACAA GTCTCTTCTC
    TACAGTGCCC CTGTTGATCC CACTGAGTGG GTTGGCCTGA GGAAGTCCTC CCCTTTACTC
    GTCTACCTGA GGAACAACCT CCTGATGTTG GCGATTTTGG CCTTCGAAGT CACTGTTTAC
    CGACATCAGG AGTACTATCG AGGCCGAAAC AACTTGACTG CTCCGGTGTC TAAAACTATC
    TTTCATGACA TCACAAGGTT ACATCTAGAT GATGGGCTTA TCAATTGTGC CAAATATTTT
    GTAAACTACT TCTTCTACAA GTTTGGCCTA GAGACCTGTT TCCTAATGTC AGTGAACGTA
    ATTGGACAGC GAATGGACTT CTACGCCATG ATTCATGCCT GTTGGCTGAT CGGTGTCTTG
    TACCGTCGCA GAAGAAAAGC CATTGCTGAG GTCTGGCCCA AGTACTGCTG CTTCCTGGCT
    TGCATCATCA CCTTCCAGTA CTTTGTCTGC ATAGGCATCC CTCCTGCCCC CTGCCGAGAT
    TACCCATGGA GGTTCAAGGG TGCCAACTTC AATGACAACA TCATCAAGTG GCTGTACTTC
    CCAGATTTCA TCGTGCGGCC CAACCCTGTG TTTCTTGTCT ATGACTTCAT GCTGCTCCTG
    TGTGCTTCCT TACAACGACA GATTTTTGAG GACGAAAACA AGGCAGCTGT GCGCATCATG
    GCCGGGGACA ATGTCGAGAT TTGCATGAAC CTTGACGCGG CCTCATTCAG CCAGCATAAC
    CCTGTACCAG ATTTCATTCA CTGCAGATCT TACTTGGACA TGTCCAAAGT GATCATCTTC
    AGCTACCTCT TCTGGTTCGT CCTCACCATC ATCTTCATTA CCGGAACGAC CAGGATCAGC
    ATCTTTTGCA TGGGTTACCT GGTGGCCTGT TTCTATTTCC TGCTCTTTGG AGGTGATCTG
    CTCCTGAAAC CCATCAAGAG CATCCTACGC TACTGGGACT GGCTGATTGC ATACAACGTG
    TTTGTGATCA CCATGAAAAA CATACTGTCA ATAGGAGCCT GTGGATACAT CGGTGCACTG
    GTGAGAAACA GCTGCTGGCT GATCCAAGCG TTCAGCCTGG CCTGCACGGT GAAAGGCTAT
    AAAATGCCTG AAGATGACTC CAGATGCAAG CTGCCCAGTG GGGAGGCAGG GATCATCTGG
    GACAGCATCT GCTTTGCGTT TCTCCTGCTA CAGAGGAGAG TCTTCATGAG TTACTATTTC
    CTGCATGTCG TGGCTGACAT TAAAGCATCC CAGATCCTGG CATCCAGAGG GGCCGAACTT
    TTCCAGGCTA CAATTGTGAA AGCTGTAAAG GCAAGAATTG AAGAAGAGAA AAAATCCATG
    GACCAGCTGA AGAGACAGAT GGATCGCATC AAAGCCCGGC AGCAAAAGTA CAAAAAGGGT
    AAGGAGAGGA TGCTGAGCTT GACCCAGGAG TCAGGGGAAG GCCAGGACAT CCAGAAAGTC
    TCAGAAGAAG ACGATGAAAT GGTCAGGAGT GGAGATTATT ATTTGTTTGA AACGGATAGT
    GAAGAGGAGG AAGAAGAGGA ACTCAAAAAG GAGGATGAAG AACCACCACG GAAGTCTGCA
    TTCCAGAGAG CTATTGGGAA GTTTGCCTCA GCCATTTTAG CCTTGCCAAA GTCTGTCATT
    AAACTTCCCA AAACTATTCT TCAGTATTTA ATCAGAGCTG CAAAGTTCGT CTACCAAGCC
    TGGATTACCG ATCCTAAAAC AGCACTCCGA CAGAGGAGGA AAGAGAAGAA GAAAATGGCC
    AGAGAAGAGC AGAAGGGAAG ACGCAAAGGA TCTGGGGAGG GTCCAGTGGA ATGGGAAGAC
    AGAGAAGATG AGCCAATCAA AAAGAAATCT GATGGACCAG ATAATATCAT CAAGAGGATA
    TTTAATATCC TGAAGTTCAC CTGGGTCCTG TTCCTGGCCA CAGTGGACAG TTTCACTACT
    TGGCTCAACT CCATCTCAAG AGAGCATATC GACATATCCA CAGTTCTGCG GATTGAAAGA
    TGCATGCTGA CCCGAGAGAT AAAGAAGGGC AACGTTCCGA CACGGGAAAG CATCCACATG
    TACTACCAGA ACCACATGAT GAACCTTTCC AGAGAATCCG GACTGGACAC AATCGACGAG
    CACTCTGGCG CTGGTTCCAG GGCCCAGGCA GCACACAGGA TGGATAGCTT AGATTCCCAT
    GACAGTATCT CCAGCTGTTA CACTGAAGCA ACCCTGCTGA TCTCAAGGCA GTCCACCCTT
    GATGATTTAG ATGGACAAGA CCCTGTTCCT AAAACAAGCG AGCGTGCTCG GCCTAGGCTC
    CGTAAAGTGT TCAGCATGGA CATGTCCTCC TCATCAGCTG ACAGTGGCAG TGTAGCATCA
    AGCGAGCCCA CACAGTGCAC CATGCTGTAT TCACGCCAAG GAACTACGGA AACCATTGAG
    GAGGTAGAGG CTGAAGCTGA GGAGGAGGTG GTAGAGAGTC TGGAGCCTGA GCTCCATGAT
    GCCGAAGAGA AGGAATATGC AGCTGACTAT GAAGCAGGGG TAGAGGAGGT CAGCCTCACT
    CCAGATGAGG AGTTGCCTCA GTTCTCAACA GATGACGGTG ACGTGGAGGC CCCGCCCTCC
    TACAGCAAGG CTGTCAGCTT TGAGCACCTC TCGTTTGCAT CACAAGATGA CTCCGGGGGC
    AAGAACCACA TGGTGGTCAG CCCTGACGAC AGCCGCACAG ACAAGCTGGA GTCCAGTATC
    TTACCGCCCC TGACTCACGA GCTGACAGCC AGCGATCTGC TCATGAGCAA GATGTTCCGT
    GATGATGAGC TTGAAGAATC TGAGAAATTC TACGTGGACC AGCCCCGGTT CTTGCTGCTA
    TTCTATGCCA TGTATAACAC TCTGGTAGCC CGCTCGGAAA TGGTGTGCTA TTTCGTGATC
    ATCCTGAACC ACATGATCTC TGCCTCCATC ATTACTCTTC TGTTGCCCAT CCTGATCTTC
    CTCTGGGCCA TGCTGTCCGT CCCCAGGCCC AGCCGGCGCT TCTGGATGAT GGCCATCGTC
    TACACTGAGG TGGCGATTGT AGTGAAATAT TTCTTCCAGT TTGGCTTCTT TCCTTGGAAT
    AAGGACTTGG AAATTTACAA GGACAGGCCC TACTTTCCAC CAAACATCAT AGGGGTGGAA
    AAGAAAGAGG GCTATGTTCT TTACGACCTC ATTCAGCTGC TGGCTCTGTT CTTCCATCGA
    TCCATTCTGA AGTGTCATGG CCTATGGGAC GAGGATGACA TTGTTGACAG TAACACAGAC
    AAGGAAGGAT CTGATGATGA GCTGTCCCTC GGTCAGGGCA GAAGAGGCTC CTCAGAGTCA
    CTGAAGTCCA TCAACTTGGC TGCCTCTGTA GAGTCCGTCC ATGTGACCTT CCCAGAGCAG
    CCAGCAGCCA TCCGGAGAAA ACGCTCCTGC AGCAGCTCGC AGATCTCCCC ACGGTCCAGC
    TTCTCTTCAA ATAGGTCCAA AAGAGGCAGC ACAAGCACCC GAAACAGCAG CCAAAAAGGA
    AGCAGTGTTT TGAGCATTAA ACAAAAAAGC AAAAGAGAAC TTTACATGGA AAAGCTTCAA
    GAACATTTAA TCAAAGCAAA GGCCTTTACC ATAAAGAAAA CTCTACAAAT CTATGTACCC
    ATTCGGCAAT TCTTCTATGA CCTCATCCAC CCAGACTACA GTGCTGTGAC TGACGTGTAT
    GTGCTGATGT TCCTGGCCGA CACCGTGGAC TTCATCATCA TCGTCTTTGG CTTCTGGGCT
    TTTGGGAAAC ACTCGGCAGC TGCAGATATC ACCTCTTCCC TATCAGAAGA CCAGGTCCCC
    GGGCCATTCC TGGTGATGGT TCTCATTCAG TTTGGAACCA TGGTGGTGGA CCGTGCACTC
    TACCTCAGGA AGACTGTGCT TGGCAAGGTC ATTTTCCAGG TCATCCTCGT GTTTGGAATT
    CACTTCTGGA TGTTCTTCAT CCTACCCAGT GTGACTGAGA GGAAATTCAG CCAGAACCTG
    GTGGCACAGC TCTGGTACTT TGTGAAATGT GTGTACTTTG GGTTGTCTGC CTACCAAATC
    CGCTGTGGCT ACCCAACACG TGTGCTCGGA AACTTCCTCA CGAAGAGCTA CAATTATGTC
    AACCTCTTCT TGTTTCAAGG GTTTCGTCTC GTCCCATTTC TGACAGAGCT GCGGGCTGTG
    ATGGACTGGG TGTGGACAGA TACAACCTTG AGCCTCTCCA GCTGGATTTG TGTGGAGGAC
    ATTTACGCAC ACATTTTCAT CTTAAAGTGC TGGCGAGAGT CGGAAAAGAG ATACCCTCAA
    CCCAGGGGCC AGAAGAAGAA GAAAGCTGTG AAGTATGGCA TGGGCGGAAT GATCATTGTC
    CTGCTCATCT GTATCGTCTG GTTTCCTCTG CTCTTCATGT CTTTGATCAA GTCTGTCGCA
    GGGGTTATTA ACCAACCCCT GGATGTGTCC GTCACAATAA CCCTGGGAGG CTATCAGCCT
    ATCTTCACAA TGAGTGCCCA GCAAAGCCAG CTGAAAGTTA TGGATGGTAC AAAGTATAAC
    GAATTCATAA AGTCTTTCGG CCCCAACAGT GGCGCTATGC AATTTCTGGA GAATTATGAA
    AGGCAAGACA TAACAGTAGC GGAACTGGAA GGGAATTCGA ATTCTTTGTG GACCATCAGC
    CCTCCCAGTA AGCAGAAAAT GATACAGGAG CTCACCGACC CCAATAGTTG CTTCTCTGTT
    GTTTTTTCAT GGAGTATCCA GAGAAACATG ACTCTGGGTG CAAAGGCTGA AATAGCAACG
    GACAAGCTTT CTTTTCCTCT CACGGATGCG ACACGAAGTA GCATAGCCAA AATGATTGCT
    GGCAATGACA CAGAAAGTTC CAACACACCA GTGACAATAG AAAAGATCTA CCCATACTAT
    GTGAAGGCAC CCAGTGACTC AAACTCAAAG CCTATAAAAC AGCTTCTGTC AGAAAACAAT
    TTCATGAATA TCACCATCAT TTTGTTCAGA GACAATGTCA CAAAGTCCAA TAGTGAGTGG
    TGGGTTCTCA ACCTGACTGG AAGTAGGATA TTCAATCAGG GATCCCAAGC CTTGGAGCTG
    GTGGTCTTCA ATGACAAAGT CAGCCCCCCA AGTCTGGGCT TCTTGGCTGG CTATGGCATC
    ATGGGATTGT ATGCATCCGT TGTCCTTGTA ATTGGGAAGT TTGTTCGCGA ATTCTTCAGT
    GGGATTTCTC ACTCTATCAT GTTTGAAGAG CTTCCAAATG TGGACAGAAT TTTGAAGTTA
    TGCACAGATA TATTTCTCGT ACGGGAGACA GGGGAACTGG AGCTGGAGGA AGACCTCTAT
    GCCAAATTGA TATTCCTGTA CAGGTCGCCA GAAACCATGA TCAAGTGGAC TAGAGAAAAA
    ACAAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa54720
    Clone ID Related Accession (Same CDS sequence) XM_017601118.1 , XM_017587879.1
    Accession Version XM_017601118.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8526bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product piezo-type mechanosensitive ion channel component 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005117.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTCCCCAT CTGCCTGGCA 
    GTAGCATGTG CATTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCG ACGATGCAAG GACACACAGG GCGGCTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCCTTT CTGCTCCTTC ACATCATTTT CCACATCACA
    CTGGCCAGCC TGGAAGCTCA GCACCGCATC GCACCCGCTT ACAACTGCTC AACTTGGGAA
    AAGACCTTCC GGCAGATCGG CTTCGAAAGC TTGAAGGGAG CTGATGCTGG CAATGGCATC
    AGGGTGTTTG TGCCTGATAT CGGGATGTTC ATTGCGAGCC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACGGAA GAAGTAGCCC AATTTAATTC CGAGTGCGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GACTCAGAAG AAGCGCTGAT TTATGAGGAG
    GACTTAGATG GGGAAGAAGG CATGGAAGGC GAGCTGGAAG AAAGCACAAA GTTAAAAATG
    TTTCGCAGGC TCGCCTCCGT CGCTTCCAAG CTTAAGGAGT TCATCGGCAA TATGATAACC
    ACGGCGGGCA AGGTGGTGGT GACCATCCTG TTGGGCTCCT CTGGCATGAT GCTGCCATCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT CTGGGTCTGT GCACCTGGTG GTCCTGGTGC
    CGAACTTTTG ACCCATTGCT GTTTGGCTGC CTCTGTGTCC TGTTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTTCAGTTCT TCCAAGAAGC AGTCCCACCC
    AATGACTACT ATGCAAGGTT GTTTGGAATC AAATCCGTTA TTCAAACAGA CTGCTCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTACAC GCTGGCCACG CTGATCCGCA TTTGGTTGCA AGAGCCTCTT
    GTGCAGGAGG AGATGTCGAA GGAAGAGGAG GGTGCACTGG ATGGCTGCTC CAATCAGAAC
    ACAGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    CTTTTATCCA TGACCCAAGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACTGTGAAC
    GGCAACCCTG TGGACTACCA CACCATCCAC CCAAGTCTGC CCATAGAGAA TGGCCCCGCC
    AAGACGGACT TGTATACTAC TCCTCAGTAC CGGTGGGAGC CCTCAGAGGA GTCCTCAGAA
    AAGAAAGAGG AGGAAGAGGA CAAGAGAGAA GACTCAGAAG GGGAAGGGAG CCAAGAGGAA
    AAACGGAGTG TGAGAATTCA TGCCATGGTC GCCGTGTTCC AGTTCATCAT GAAGCAGAGT
    TACATCTGTG CCCTCATCGC CATGATGGCA TGGAGCATCA CCTACCACAG CTGGCTGACA
    TTTGTGCTGT TGATCTGGTC GTGCACTCTC TGGATGATTC GCAACAGGAG GAAGTACGCC
    ATGATCAGCT CTCCGTTCAT GGTTGTCTAT GCCAATCTGC TGCTGGTACT GCAGTACATA
    TGGAGCTTTG AACTTCCAGA AATCAAGAAA GTCCCTGGAT TTCTAGAAAA GAAAGAGCCT
    GGAGAGCTTG CCTCAAAGAT CCTCTTCACC ATCACATTTT GGCTGCTCCT GAGGCAGCAT
    CTCACAGAGC AGAAGGCCCT TCAGGAAAAG GAAGCACTTC TGTCTGAAGT CAAAATTGGA
    AGTCAAGAAC TTGACGAAAA GGATGAGGAA CTCCAAGATA TACAAGTGGA AGGAGAGCCC
    ATAGAGAAGG AAGAAGAGGA GGAGGAAATA AAGGAAGACA GACATGAGGT AAAGAAGGAA
    GAGGAAGAGG AAGAGGAAGA TGACGATGAC CAGGACATCA TGAAGGTTCT GGGCAATCTG
    GTGGTGGCCC TCTTCATCAA GTATTGGATC TACGTCTGCG GGGGCATGTT CTTTTTTGTC
    AGCTTTGAGG GGAAAATCGT CATGTACAAA ATCATCTACA TGGTGCTGTT CCTGTTCTGT
    GTGGCCTTGT ACCAGGTGCA CTACGAGTGG TGGAGGAAGA TCCTGAAATA CTTCTGGATG
    TCGGTGGTCA TTTACACCAT GCTGGTGCTC ATCTTCATCT ACACTTACCA GTTTGAGAAC
    TTCCCAGGCC TGTGGCAAAA CATGACCGGG TTAAAGAAAG AGAAGCTTGA GGATCTGGGC
    CTGAAGCAGT TCACTGTGGC CGAACTGTTC ACACGAATAT TCATCCCCAC CTCCTTTCTG
    CTGGTCTGCA TCCTGCACCT CCACTATTTC CATGACCGGT TCCTGGAACT CACAGACCTC
    AAGTCCATCC CCAGCAAAGA AGACAACACC ATCTACAGGT TAGCCCACCC TGAAGGAAGC
    CTCCCAGACC TAGCCATTAT GAACATGACG GCCAGCCTCG ATAAGCCTGA GAGGCAGAAG
    TTGGCAGAGC CTGGGGAGGA GAGGACAGAA GAATGTGCCA AGAAGGCAGA GAAGGGTGAG
    GCTGGGAAAG ACAGTGATGA GTCTGAGGAG GAGGAAGGTG AGGAAGAGGA ATCCGAGGAG
    GAAGAATCCT CAGATCTCAG GAACAAGTGG CACCTCGTGA TTGACCGGCT CACTGTGCTC
    TTTCTAAAGT TCCTGGAGTA TTTCCACAAG TTGCAGGTGT TTATGTGGTG GATCTTGGAG
    CTGCACATCA TCAAAATCGT CTCATCGTAC ATTATCTGGG TGACTGTCAA AGAGGTGTCT
    CTGTTCAACT ATGTATTTTT GATTTCTTGG GCATTTGCTC TGCCATATGC CAAACTCCGC
    CGTGTGGCTT CCAGTGTCTG CACTGTCTGG ACGTGTGTGA TCATCGTCTG CAAAATGTTG
    TACCAGCTGC AAACCATTAA GCCTGAGAAC TTTTCTGTCA ACTGCTCCTT GCCCAATGAA
    AATCAAACCA ACATACCCCT TAATGAGTTG AACAAGTCTC TTCTCTACAG TGCCCCTGTT
    GATCCCACTG AGTGGGTTGG CCTGAGGAAG TCCTCCCCTT TACTCGTCTA CCTGAGGAAC
    AACCTCCTGA TGTTGGCGAT TTTGGCCTTC GAAGTCACTG TTTACCGACA TCAGGAGTAC
    TATCGAGGCC GAAACAACTT GACTGCTCCG GTGTCTAAAA CTATCTTTCA TGACATCACA
    AGGTTACATC TAGATGATGG GCTTATCAAT TGTGCCAAAT ATTTTGTAAA CTACTTCTTC
    TACAAGTTTG GCCTAGAGAC CTGTTTCCTA ATGTCAGTGA ACGTAATTGG ACAGCGAATG
    GACTTCTACG CCATGATTCA TGCCTGTTGG CTGATCGGTG TCTTGTACCG TCGCAGAAGA
    AAAGCCATTG CTGAGGTCTG GCCCAAGTAC TGCTGCTTCC TGGCTTGCAT CATCACCTTC
    CAGTACTTTG TCTGCATAGG CATCCCTCCT GCCCCCTGCC GAGATTACCC ATGGAGGTTC
    AAGGGTGCCA ACTTCAATGA CAACATCATC AAGTGGCTGT ACTTCCCAGA TTTCATCGTG
    CGGCCCAACC CTGTGTTTCT TGTCTATGAC TTCATGCTGC TCCTGTGTGC TTCCTTACAA
    CGACAGATTT TTGAGGACGA AAACAAGGCA GCTGTGCGCA TCATGGCCGG GGACAATGTC
    GAGATTTGCA TGAACCTTGA CGCGGCCTCA TTCAGCCAGC ATAACCCTGT ACCAGATTTC
    ATTCACTGCA GATCTTACTT GGACATGTCC AAAGTGATCA TCTTCAGCTA CCTCTTCTGG
    TTCGTCCTCA CCATCATCTT CATTACCGGA ACGACCAGGA TCAGCATCTT TTGCATGGGT
    TACCTGGTGG CCTGTTTCTA TTTCCTGCTC TTTGGAGGTG ATCTGCTCCT GAAACCCATC
    AAGAGCATCC TACGCTACTG GGACTGGCTG ATTGCATACA ACGTGTTTGT GATCACCATG
    AAAAACATAC TGTCAATAGG AGCCTGTGGA TACATCGGTG CACTGGTGAG AAACAGCTGC
    TGGCTGATCC AAGCGTTCAG CCTGGCCTGC ACGGTGAAAG GCTATAAAAT GCCTGAAGAT
    GACTCCAGAT GCAAGCTGCC CAGTGGGGAG GCAGGGATCA TCTGGGACAG CATCTGCTTT
    GCGTTTCTCC TGCTACAGAG GAGAGTCTTC ATGAGTTACT ATTTCCTGCA TGTCGTGGCT
    GACATTAAAG CATCCCAGAT CCTGGCATCC AGAGGGGCCG AACTTTTCCA GGCTACAATT
    GTGAAAGCTG TAAAGGCAAG AATTGAAGAA GAGAAAAAAT CCATGGACCA GCTGAAGAGA
    CAGATGGATC GCATCAAAGC CCGGCAGCAA AAGTACAAAA AGGGTAAGGA GAGGATGCTG
    AGCTTGACCC AGGAGTCAGG GGAAGGCCAG GACATCCAGA AAGTCTCAGA AGAAGACGAT
    GAAAGAGAAG CAGACAAACA GAAAGCCAAG GGCAAAAAAA AGCAGTGGTG GCGGCCTTGG
    GTTGATCATG CTTCCATGGT CAGGAGTGGA GATTATTATT TGTTTGAAAC GGATAGTGAA
    GAGGAGGAAG AAGAGGAACT CAAAAAGGAG GATGAAGAAC CACCACGGAA GTCTGCATTC
    CAGAGAGCTA TTGGGAAGTT TGCCTCAGCC ATTTTAGCCT TGCCAAAGTC TGTCATTAAA
    CTTCCCAAAA CTATTCTTCA GTATTTAATC AGAGCTGCAA AGTTCGTCTA CCAAGCCTGG
    ATTACCGATC CTAAAACAGC ACTCCGACAG AGGAGGAAAG AGAAGAAGAA AATGGCCAGA
    GAAGAGCAGA AGGGAAGACG CAAAGGATCT GGGGAGGGTC CAGTGGAATG GGAAGACAGA
    GAAGATGAGC CAATCAAAAA GAAATCTGAT GGACCAGATA ATATCATCAA GAGGATATTT
    AATATCCTGA AGTTCACCTG GGTCCTGTTC CTGGCCACAG TGGACAGTTT CACTACTTGG
    CTCAACTCCA TCTCAAGAGA GCATATCGAC ATATCCACAG TTCTGCGGAT TGAAAGATGC
    ATGCTGACCC GAGAGATAAA GAAGGGCAAC GTTCCGACAC GGGAAAGCAT CCACATGTAC
    TACCAGAACC ACATGATGAA CCTTTCCAGA GAATCCGGAC TGGACACAAT CGACGAGCAC
    TCTGGCGCTG GTTCCAGGGC CCAGGCAGCA CACAGGATGG ATAGCTTAGA TTCCCATGAC
    AGTATCTCCA GCTGTTACAC TGAAGCAACC CTGCTGATCT CAAGGCAGTC CACCCTTGAT
    GATTTAGATG GACAAGACCC TGTTCCTAAA ACAAGCGAGC GTGCTCGGCC TAGGCTCCGT
    AAAGTGTTCA GCATGGACAT GTCCTCCTCA TCAGCTGACA GTGGCAGTGT AGCATCAAGC
    GAGCCCACAC AGTGCACCAT GCTGTATTCA CGCCAAGGAA CTACGGAAAC CATTGAGGAG
    GTAGAGGCTG AAGCTGAGGA GGAGGTGGTA GAGAGTCTGG AGCCTGAGCT CCATGATGCC
    GAAGAGAAGG AATATGCAGC TGACTATGAA GCAGGGGTAG AGGAGGTCAG CCTCACTCCA
    GATGAGGAGT TGCCTCAGTT CTCAACAGAT GACGGTGACG TGGAGGCCCC GCCCTCCTAC
    AGCAAGGCTG TCAGCTTTGA GCACCTCTCG TTTGCATCAC AAGATGACTC CGGGGGCAAG
    AACCACATGG TGGTCAGCCC TGACGACAGC CGCACAGACA AGCTGGAGTC CAGTATCTTA
    CCGCCCCTGA CTCACGAGCT GACAGCCAGC GATCTGCTCA TGAGCAAGAT GTTCCGTGAT
    GATGAGCTTG AAGAATCTGA GAAATTCTAC GTGGACCAGC CCCGGTTCTT GCTGCTATTC
    TATGCCATGT ATAACACTCT GGTAGCCCGC TCGGAAATGG TGTGCTATTT CGTGATCATC
    CTGAACCACA TGATCTCTGC CTCCATCATT ACTCTTCTGT TGCCCATCCT GATCTTCCTC
    TGGGCCATGC TGTCCGTCCC CAGGCCCAGC CGGCGCTTCT GGATGATGGC CATCGTCTAC
    ACTGAGGTGG CGATTGTAGT GAAATATTTC TTCCAGTTTG GCTTCTTTCC TTGGAATAAG
    GACTTGGAAA TTTACAAGGA CAGGCCCTAC TTTCCACCAA ACATCATAGG GGTGGAAAAG
    AAAGAGGGCT ATGTTCTTTA CGACCTCATT CAGCTGCTGG CTCTGTTCTT CCATCGATCC
    ATTCTGAAGT GTCATGGCCT ATGGGACGAG GATGACATTG TTGACAGTAA CACAGACAAG
    GAAGGATCTG ATGATGAGCT GTCCCTCGGT CAGGGCAGAA GAGGCTCCTC AGAGTCACTG
    AAGTCCATCA ACTTGGCTGC CTCTGTAGAG TCCGTCCATG TGACCTTCCC AGAGCAGCCA
    GCAGCCATCC GGAGAAAACG CTCCTGCAGC AGCTCGCAGA TCTCCCCACG GTCCAGCTTC
    TCTTCAAATA GGTCCAAAAG AGGCAGCACA AGCACCCGAA ACAGCAGCCA AAAAGGAAGC
    AGTGTTTTGA GCATTAAACA AAAAAGCAAA AGAGAACTTT ACATGGAAAA GCTTCAAGAA
    CATTTAATCA AAGCAAAGGC CTTTACCATA AAGAAAACTC TACAAATCTA TGTACCCATT
    CGGCAATTCT TCTATGACCT CATCCACCCA GACTACAGTG CTGTGACTGA CGTGTATGTG
    CTGATGTTCC TGGCCGACAC CGTGGACTTC ATCATCATCG TCTTTGGCTT CTGGGCTTTT
    GGGAAACACT CGGCAGCTGC AGATATCACC TCTTCCCTAT CAGAAGACCA GGTCCCCGGG
    CCATTCCTGG TGATGGTTCT CATTCAGTTT GGAACCATGG TGGTGGACCG TGCACTCTAC
    CTCAGGAAGA CTGTGCTTGG CAAGGTCATT TTCCAGGTCA TCCTCGTGTT TGGAATTCAC
    TTCTGGATGT TCTTCATCCT ACCCAGTGTG ACTGAGAGGA AATTCAGCCA GAACCTGGTG
    GCACAGCTCT GGTACTTTGT GAAATGTGTG TACTTTGGGT TGTCTGCCTA CCAAATCCGC
    TGTGGCTACC CAACACGTGT GCTCGGAAAC TTCCTCACGA AGAGCTACAA TTATGTCAAC
    CTCTTCTTGT TTCAAGGGTT TCGTCTCGTC CCATTTCTGA CAGAGCTGCG GGCTGTGATG
    GACTGGGTGT GGACAGATAC AACCTTGAGC CTCTCCAGCT GGATTTGTGT GGAGGACATT
    TACGCACACA TTTTCATCTT AAAGTGCTGG CGAGAGTCGG AAAAGAGATA CCCTCAACCC
    AGGGGCCAGA AGAAGAAGAA AGCTGTGAAG TATGGCATGG GCGGAATGAT CATTGTCCTG
    CTCATCTGTA TCGTCTGGTT TCCTCTGCTC TTCATGTCTT TGATCAAGTC TGTCGCAGGG
    GTTATTAACC AACCCCTGGA TGTGTCCGTC ACAATAACCC TGGGAGGCTA TCAGCCTATC
    TTCACAATGA GTGCCCAGCA AAGCCAGCTG AAAGTTATGG ATGGTACAAA GTATAACGAA
    TTCATAAAGT CTTTCGGCCC CAACAGTGGC GCTATGCAAT TTCTGGAGAA TTATGAAAGG
    CAAGACATAA CAGTAGCGGA ACTGGAAGGG AATTCGAATT CTTTGTGGAC CATCAGCCCT
    CCCAGTAAGC AGAAAATGAT ACAGGAGCTC ACCGACCCCA ATAGTTGCTT CTCTGTTGTT
    TTTTCATGGA GTATCCAGAG AAACATGACT CTGGGTGCAA AGGCTGAAAT AGCAACGGAC
    AAGCTTTCTT TTCCTCTCAC GGATGCGACA CGAAGTAGCA TAGCCAAAAT GATTGCTGGC
    AATGACACAG AAAGTTCCAA CACACCAGTG ACAATAGAAA AGATCTACCC ATACTATGTG
    AAGGCACCCA GTGACTCAAA CTCAAAGCCT ATAAAACAGC TTCTGTCAGA AAACAATTTC
    ATGAATATCA CCATCATTTT GTTCAGAGAC AATGTCACAA AGTCCAATAG TGAGTGGTGG
    GTTCTCAACC TGACTGGAAG TAGGATATTC AATCAGGGAT CCCAAGCCTT GGAGCTGGTG
    GTCTTCAATG ACAAAGTCAG CCCCCCAAGT CTGGGCTTCT TGGCTGGCTA TGGCATCATG
    GGATTGTATG CATCCGTTGT CCTTGTAATT GGGAAGTTTG TTCGCGAATT CTTCAGTGGG
    ATTTCTCACT CTATCATGTT TGAAGAGCTT CCAAATGTGG ACAGAATTTT GAAGTTATGC
    ACAGATATAT TTCTCGTACG GGAGACAGGG GAACTGGAGC TGGAGGAAGA CCTCTATGCC
    AAATTGATAT TCCTGTACAG GTCGCCAGAA ACCATGATCA AGTGGACTAG AGAAAAAACA
    AATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017456607.1
    CDS940..9465
    Translation

    Target ORF information:

    RefSeq Version XM_017601118.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017601118.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTCCCCAT CTGCCTGGCA 
    GTAGCATGTG CATTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCG ACGATGCAAG GACACACAGG GCGGCTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCCTTT CTGCTCCTTC ACATCATTTT CCACATCACA
    CTGGCCAGCC TGGAAGCTCA GCACCGCATC GCACCCGCTT ACAACTGCTC AACTTGGGAA
    AAGACCTTCC GGCAGATCGG CTTCGAAAGC TTGAAGGGAG CTGATGCTGG CAATGGCATC
    AGGGTGTTTG TGCCTGATAT CGGGATGTTC ATTGCGAGCC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACGGAA GAAGTAGCCC AATTTAATTC CGAGTGCGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GACTCAGAAG AAGCGCTGAT TTATGAGGAG
    GACTTAGATG GGGAAGAAGG CATGGAAGGC GAGCTGGAAG AAAGCACAAA GTTAAAAATG
    TTTCGCAGGC TCGCCTCCGT CGCTTCCAAG CTTAAGGAGT TCATCGGCAA TATGATAACC
    ACGGCGGGCA AGGTGGTGGT GACCATCCTG TTGGGCTCCT CTGGCATGAT GCTGCCATCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT CTGGGTCTGT GCACCTGGTG GTCCTGGTGC
    CGAACTTTTG ACCCATTGCT GTTTGGCTGC CTCTGTGTCC TGTTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTTCAGTTCT TCCAAGAAGC AGTCCCACCC
    AATGACTACT ATGCAAGGTT GTTTGGAATC AAATCCGTTA TTCAAACAGA CTGCTCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTACAC GCTGGCCACG CTGATCCGCA TTTGGTTGCA AGAGCCTCTT
    GTGCAGGAGG AGATGTCGAA GGAAGAGGAG GGTGCACTGG ATGGCTGCTC CAATCAGAAC
    ACAGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    CTTTTATCCA TGACCCAAGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACTGTGAAC
    GGCAACCCTG TGGACTACCA CACCATCCAC CCAAGTCTGC CCATAGAGAA TGGCCCCGCC
    AAGACGGACT TGTATACTAC TCCTCAGTAC CGGTGGGAGC CCTCAGAGGA GTCCTCAGAA
    AAGAAAGAGG AGGAAGAGGA CAAGAGAGAA GACTCAGAAG GGGAAGGGAG CCAAGAGGAA
    AAACGGAGTG TGAGAATTCA TGCCATGGTC GCCGTGTTCC AGTTCATCAT GAAGCAGAGT
    TACATCTGTG CCCTCATCGC CATGATGGCA TGGAGCATCA CCTACCACAG CTGGCTGACA
    TTTGTGCTGT TGATCTGGTC GTGCACTCTC TGGATGATTC GCAACAGGAG GAAGTACGCC
    ATGATCAGCT CTCCGTTCAT GGTTGTCTAT GCCAATCTGC TGCTGGTACT GCAGTACATA
    TGGAGCTTTG AACTTCCAGA AATCAAGAAA GTCCCTGGAT TTCTAGAAAA GAAAGAGCCT
    GGAGAGCTTG CCTCAAAGAT CCTCTTCACC ATCACATTTT GGCTGCTCCT GAGGCAGCAT
    CTCACAGAGC AGAAGGCCCT TCAGGAAAAG GAAGCACTTC TGTCTGAAGT CAAAATTGGA
    AGTCAAGAAC TTGACGAAAA GGATGAGGAA CTCCAAGATA TACAAGTGGA AGGAGAGCCC
    ATAGAGAAGG AAGAAGAGGA GGAGGAAATA AAGGAAGACA GACATGAGGT AAAGAAGGAA
    GAGGAAGAGG AAGAGGAAGA TGACGATGAC CAGGACATCA TGAAGGTTCT GGGCAATCTG
    GTGGTGGCCC TCTTCATCAA GTATTGGATC TACGTCTGCG GGGGCATGTT CTTTTTTGTC
    AGCTTTGAGG GGAAAATCGT CATGTACAAA ATCATCTACA TGGTGCTGTT CCTGTTCTGT
    GTGGCCTTGT ACCAGGTGCA CTACGAGTGG TGGAGGAAGA TCCTGAAATA CTTCTGGATG
    TCGGTGGTCA TTTACACCAT GCTGGTGCTC ATCTTCATCT ACACTTACCA GTTTGAGAAC
    TTCCCAGGCC TGTGGCAAAA CATGACCGGG TTAAAGAAAG AGAAGCTTGA GGATCTGGGC
    CTGAAGCAGT TCACTGTGGC CGAACTGTTC ACACGAATAT TCATCCCCAC CTCCTTTCTG
    CTGGTCTGCA TCCTGCACCT CCACTATTTC CATGACCGGT TCCTGGAACT CACAGACCTC
    AAGTCCATCC CCAGCAAAGA AGACAACACC ATCTACAGGT TAGCCCACCC TGAAGGAAGC
    CTCCCAGACC TAGCCATTAT GAACATGACG GCCAGCCTCG ATAAGCCTGA GAGGCAGAAG
    TTGGCAGAGC CTGGGGAGGA GAGGACAGAA GAATGTGCCA AGAAGGCAGA GAAGGGTGAG
    GCTGGGAAAG ACAGTGATGA GTCTGAGGAG GAGGAAGGTG AGGAAGAGGA ATCCGAGGAG
    GAAGAATCCT CAGATCTCAG GAACAAGTGG CACCTCGTGA TTGACCGGCT CACTGTGCTC
    TTTCTAAAGT TCCTGGAGTA TTTCCACAAG TTGCAGGTGT TTATGTGGTG GATCTTGGAG
    CTGCACATCA TCAAAATCGT CTCATCGTAC ATTATCTGGG TGACTGTCAA AGAGGTGTCT
    CTGTTCAACT ATGTATTTTT GATTTCTTGG GCATTTGCTC TGCCATATGC CAAACTCCGC
    CGTGTGGCTT CCAGTGTCTG CACTGTCTGG ACGTGTGTGA TCATCGTCTG CAAAATGTTG
    TACCAGCTGC AAACCATTAA GCCTGAGAAC TTTTCTGTCA ACTGCTCCTT GCCCAATGAA
    AATCAAACCA ACATACCCCT TAATGAGTTG AACAAGTCTC TTCTCTACAG TGCCCCTGTT
    GATCCCACTG AGTGGGTTGG CCTGAGGAAG TCCTCCCCTT TACTCGTCTA CCTGAGGAAC
    AACCTCCTGA TGTTGGCGAT TTTGGCCTTC GAAGTCACTG TTTACCGACA TCAGGAGTAC
    TATCGAGGCC GAAACAACTT GACTGCTCCG GTGTCTAAAA CTATCTTTCA TGACATCACA
    AGGTTACATC TAGATGATGG GCTTATCAAT TGTGCCAAAT ATTTTGTAAA CTACTTCTTC
    TACAAGTTTG GCCTAGAGAC CTGTTTCCTA ATGTCAGTGA ACGTAATTGG ACAGCGAATG
    GACTTCTACG CCATGATTCA TGCCTGTTGG CTGATCGGTG TCTTGTACCG TCGCAGAAGA
    AAAGCCATTG CTGAGGTCTG GCCCAAGTAC TGCTGCTTCC TGGCTTGCAT CATCACCTTC
    CAGTACTTTG TCTGCATAGG CATCCCTCCT GCCCCCTGCC GAGATTACCC ATGGAGGTTC
    AAGGGTGCCA ACTTCAATGA CAACATCATC AAGTGGCTGT ACTTCCCAGA TTTCATCGTG
    CGGCCCAACC CTGTGTTTCT TGTCTATGAC TTCATGCTGC TCCTGTGTGC TTCCTTACAA
    CGACAGATTT TTGAGGACGA AAACAAGGCA GCTGTGCGCA TCATGGCCGG GGACAATGTC
    GAGATTTGCA TGAACCTTGA CGCGGCCTCA TTCAGCCAGC ATAACCCTGT ACCAGATTTC
    ATTCACTGCA GATCTTACTT GGACATGTCC AAAGTGATCA TCTTCAGCTA CCTCTTCTGG
    TTCGTCCTCA CCATCATCTT CATTACCGGA ACGACCAGGA TCAGCATCTT TTGCATGGGT
    TACCTGGTGG CCTGTTTCTA TTTCCTGCTC TTTGGAGGTG ATCTGCTCCT GAAACCCATC
    AAGAGCATCC TACGCTACTG GGACTGGCTG ATTGCATACA ACGTGTTTGT GATCACCATG
    AAAAACATAC TGTCAATAGG AGCCTGTGGA TACATCGGTG CACTGGTGAG AAACAGCTGC
    TGGCTGATCC AAGCGTTCAG CCTGGCCTGC ACGGTGAAAG GCTATAAAAT GCCTGAAGAT
    GACTCCAGAT GCAAGCTGCC CAGTGGGGAG GCAGGGATCA TCTGGGACAG CATCTGCTTT
    GCGTTTCTCC TGCTACAGAG GAGAGTCTTC ATGAGTTACT ATTTCCTGCA TGTCGTGGCT
    GACATTAAAG CATCCCAGAT CCTGGCATCC AGAGGGGCCG AACTTTTCCA GGCTACAATT
    GTGAAAGCTG TAAAGGCAAG AATTGAAGAA GAGAAAAAAT CCATGGACCA GCTGAAGAGA
    CAGATGGATC GCATCAAAGC CCGGCAGCAA AAGTACAAAA AGGGTAAGGA GAGGATGCTG
    AGCTTGACCC AGGAGTCAGG GGAAGGCCAG GACATCCAGA AAGTCTCAGA AGAAGACGAT
    GAAAGAGAAG CAGACAAACA GAAAGCCAAG GGCAAAAAAA AGCAGTGGTG GCGGCCTTGG
    GTTGATCATG CTTCCATGGT CAGGAGTGGA GATTATTATT TGTTTGAAAC GGATAGTGAA
    GAGGAGGAAG AAGAGGAACT CAAAAAGGAG GATGAAGAAC CACCACGGAA GTCTGCATTC
    CAGAGAGCTA TTGGGAAGTT TGCCTCAGCC ATTTTAGCCT TGCCAAAGTC TGTCATTAAA
    CTTCCCAAAA CTATTCTTCA GTATTTAATC AGAGCTGCAA AGTTCGTCTA CCAAGCCTGG
    ATTACCGATC CTAAAACAGC ACTCCGACAG AGGAGGAAAG AGAAGAAGAA AATGGCCAGA
    GAAGAGCAGA AGGGAAGACG CAAAGGATCT GGGGAGGGTC CAGTGGAATG GGAAGACAGA
    GAAGATGAGC CAATCAAAAA GAAATCTGAT GGACCAGATA ATATCATCAA GAGGATATTT
    AATATCCTGA AGTTCACCTG GGTCCTGTTC CTGGCCACAG TGGACAGTTT CACTACTTGG
    CTCAACTCCA TCTCAAGAGA GCATATCGAC ATATCCACAG TTCTGCGGAT TGAAAGATGC
    ATGCTGACCC GAGAGATAAA GAAGGGCAAC GTTCCGACAC GGGAAAGCAT CCACATGTAC
    TACCAGAACC ACATGATGAA CCTTTCCAGA GAATCCGGAC TGGACACAAT CGACGAGCAC
    TCTGGCGCTG GTTCCAGGGC CCAGGCAGCA CACAGGATGG ATAGCTTAGA TTCCCATGAC
    AGTATCTCCA GCTGTTACAC TGAAGCAACC CTGCTGATCT CAAGGCAGTC CACCCTTGAT
    GATTTAGATG GACAAGACCC TGTTCCTAAA ACAAGCGAGC GTGCTCGGCC TAGGCTCCGT
    AAAGTGTTCA GCATGGACAT GTCCTCCTCA TCAGCTGACA GTGGCAGTGT AGCATCAAGC
    GAGCCCACAC AGTGCACCAT GCTGTATTCA CGCCAAGGAA CTACGGAAAC CATTGAGGAG
    GTAGAGGCTG AAGCTGAGGA GGAGGTGGTA GAGAGTCTGG AGCCTGAGCT CCATGATGCC
    GAAGAGAAGG AATATGCAGC TGACTATGAA GCAGGGGTAG AGGAGGTCAG CCTCACTCCA
    GATGAGGAGT TGCCTCAGTT CTCAACAGAT GACGGTGACG TGGAGGCCCC GCCCTCCTAC
    AGCAAGGCTG TCAGCTTTGA GCACCTCTCG TTTGCATCAC AAGATGACTC CGGGGGCAAG
    AACCACATGG TGGTCAGCCC TGACGACAGC CGCACAGACA AGCTGGAGTC CAGTATCTTA
    CCGCCCCTGA CTCACGAGCT GACAGCCAGC GATCTGCTCA TGAGCAAGAT GTTCCGTGAT
    GATGAGCTTG AAGAATCTGA GAAATTCTAC GTGGACCAGC CCCGGTTCTT GCTGCTATTC
    TATGCCATGT ATAACACTCT GGTAGCCCGC TCGGAAATGG TGTGCTATTT CGTGATCATC
    CTGAACCACA TGATCTCTGC CTCCATCATT ACTCTTCTGT TGCCCATCCT GATCTTCCTC
    TGGGCCATGC TGTCCGTCCC CAGGCCCAGC CGGCGCTTCT GGATGATGGC CATCGTCTAC
    ACTGAGGTGG CGATTGTAGT GAAATATTTC TTCCAGTTTG GCTTCTTTCC TTGGAATAAG
    GACTTGGAAA TTTACAAGGA CAGGCCCTAC TTTCCACCAA ACATCATAGG GGTGGAAAAG
    AAAGAGGGCT ATGTTCTTTA CGACCTCATT CAGCTGCTGG CTCTGTTCTT CCATCGATCC
    ATTCTGAAGT GTCATGGCCT ATGGGACGAG GATGACATTG TTGACAGTAA CACAGACAAG
    GAAGGATCTG ATGATGAGCT GTCCCTCGGT CAGGGCAGAA GAGGCTCCTC AGAGTCACTG
    AAGTCCATCA ACTTGGCTGC CTCTGTAGAG TCCGTCCATG TGACCTTCCC AGAGCAGCCA
    GCAGCCATCC GGAGAAAACG CTCCTGCAGC AGCTCGCAGA TCTCCCCACG GTCCAGCTTC
    TCTTCAAATA GGTCCAAAAG AGGCAGCACA AGCACCCGAA ACAGCAGCCA AAAAGGAAGC
    AGTGTTTTGA GCATTAAACA AAAAAGCAAA AGAGAACTTT ACATGGAAAA GCTTCAAGAA
    CATTTAATCA AAGCAAAGGC CTTTACCATA AAGAAAACTC TACAAATCTA TGTACCCATT
    CGGCAATTCT TCTATGACCT CATCCACCCA GACTACAGTG CTGTGACTGA CGTGTATGTG
    CTGATGTTCC TGGCCGACAC CGTGGACTTC ATCATCATCG TCTTTGGCTT CTGGGCTTTT
    GGGAAACACT CGGCAGCTGC AGATATCACC TCTTCCCTAT CAGAAGACCA GGTCCCCGGG
    CCATTCCTGG TGATGGTTCT CATTCAGTTT GGAACCATGG TGGTGGACCG TGCACTCTAC
    CTCAGGAAGA CTGTGCTTGG CAAGGTCATT TTCCAGGTCA TCCTCGTGTT TGGAATTCAC
    TTCTGGATGT TCTTCATCCT ACCCAGTGTG ACTGAGAGGA AATTCAGCCA GAACCTGGTG
    GCACAGCTCT GGTACTTTGT GAAATGTGTG TACTTTGGGT TGTCTGCCTA CCAAATCCGC
    TGTGGCTACC CAACACGTGT GCTCGGAAAC TTCCTCACGA AGAGCTACAA TTATGTCAAC
    CTCTTCTTGT TTCAAGGGTT TCGTCTCGTC CCATTTCTGA CAGAGCTGCG GGCTGTGATG
    GACTGGGTGT GGACAGATAC AACCTTGAGC CTCTCCAGCT GGATTTGTGT GGAGGACATT
    TACGCACACA TTTTCATCTT AAAGTGCTGG CGAGAGTCGG AAAAGAGATA CCCTCAACCC
    AGGGGCCAGA AGAAGAAGAA AGCTGTGAAG TATGGCATGG GCGGAATGAT CATTGTCCTG
    CTCATCTGTA TCGTCTGGTT TCCTCTGCTC TTCATGTCTT TGATCAAGTC TGTCGCAGGG
    GTTATTAACC AACCCCTGGA TGTGTCCGTC ACAATAACCC TGGGAGGCTA TCAGCCTATC
    TTCACAATGA GTGCCCAGCA AAGCCAGCTG AAAGTTATGG ATGGTACAAA GTATAACGAA
    TTCATAAAGT CTTTCGGCCC CAACAGTGGC GCTATGCAAT TTCTGGAGAA TTATGAAAGG
    CAAGACATAA CAGTAGCGGA ACTGGAAGGG AATTCGAATT CTTTGTGGAC CATCAGCCCT
    CCCAGTAAGC AGAAAATGAT ACAGGAGCTC ACCGACCCCA ATAGTTGCTT CTCTGTTGTT
    TTTTCATGGA GTATCCAGAG AAACATGACT CTGGGTGCAA AGGCTGAAAT AGCAACGGAC
    AAGCTTTCTT TTCCTCTCAC GGATGCGACA CGAAGTAGCA TAGCCAAAAT GATTGCTGGC
    AATGACACAG AAAGTTCCAA CACACCAGTG ACAATAGAAA AGATCTACCC ATACTATGTG
    AAGGCACCCA GTGACTCAAA CTCAAAGCCT ATAAAACAGC TTCTGTCAGA AAACAATTTC
    ATGAATATCA CCATCATTTT GTTCAGAGAC AATGTCACAA AGTCCAATAG TGAGTGGTGG
    GTTCTCAACC TGACTGGAAG TAGGATATTC AATCAGGGAT CCCAAGCCTT GGAGCTGGTG
    GTCTTCAATG ACAAAGTCAG CCCCCCAAGT CTGGGCTTCT TGGCTGGCTA TGGCATCATG
    GGATTGTATG CATCCGTTGT CCTTGTAATT GGGAAGTTTG TTCGCGAATT CTTCAGTGGG
    ATTTCTCACT CTATCATGTT TGAAGAGCTT CCAAATGTGG ACAGAATTTT GAAGTTATGC
    ACAGATATAT TTCTCGTACG GGAGACAGGG GAACTGGAGC TGGAGGAAGA CCTCTATGCC
    AAATTGATAT TCCTGTACAG GTCGCCAGAA ACCATGATCA AGTGGACTAG AGAAAAAACA
    AATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa54720
    Clone ID Related Accession (Same CDS sequence) XM_017601118.1 , XM_017587879.1
    Accession Version XM_017587879.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8526bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product piezo-type mechanosensitive ion channel component 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000086.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
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    1801
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    5941
    6001
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    6181
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    6301
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    6421
    6481
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    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTCCCCAT CTGCCTGGCA 
    GTAGCATGTG CATTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCG ACGATGCAAG GACACACAGG GCGGCTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCCTTT CTGCTCCTTC ACATCATTTT CCACATCACA
    CTGGCCAGCC TGGAAGCTCA GCACCGCATC GCACCCGCTT ACAACTGCTC AACTTGGGAA
    AAGACCTTCC GGCAGATCGG CTTCGAAAGC TTGAAGGGAG CTGATGCTGG CAATGGCATC
    AGGGTGTTTG TGCCTGATAT CGGGATGTTC ATTGCGAGCC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACGGAA GAAGTAGCCC AATTTAATTC CGAGTGCGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GACTCAGAAG AAGCGCTGAT TTATGAGGAG
    GACTTAGATG GGGAAGAAGG CATGGAAGGC GAGCTGGAAG AAAGCACAAA GTTAAAAATG
    TTTCGCAGGC TCGCCTCCGT CGCTTCCAAG CTTAAGGAGT TCATCGGCAA TATGATAACC
    ACGGCGGGCA AGGTGGTGGT GACCATCCTG TTGGGCTCCT CTGGCATGAT GCTGCCATCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT CTGGGTCTGT GCACCTGGTG GTCCTGGTGC
    CGAACTTTTG ACCCATTGCT GTTTGGCTGC CTCTGTGTCC TGTTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTTCAGTTCT TCCAAGAAGC AGTCCCACCC
    AATGACTACT ATGCAAGGTT GTTTGGAATC AAATCCGTTA TTCAAACAGA CTGCTCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTACAC GCTGGCCACG CTGATCCGCA TTTGGTTGCA AGAGCCTCTT
    GTGCAGGAGG AGATGTCGAA GGAAGAGGAG GGTGCACTGG ATGGCTGCTC CAATCAGAAC
    ACAGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    CTTTTATCCA TGACCCAAGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACTGTGAAC
    GGCAACCCTG TGGACTACCA CACCATCCAC CCAAGTCTGC CCATAGAGAA TGGCCCCGCC
    AAGACGGACT TGTATACTAC TCCTCAGTAC CGGTGGGAGC CCTCAGAGGA GTCCTCAGAA
    AAGAAAGAGG AGGAAGAGGA CAAGAGAGAA GACTCAGAAG GGGAAGGGAG CCAAGAGGAA
    AAACGGAGTG TGAGAATTCA TGCCATGGTC GCCGTGTTCC AGTTCATCAT GAAGCAGAGT
    TACATCTGTG CCCTCATCGC CATGATGGCA TGGAGCATCA CCTACCACAG CTGGCTGACA
    TTTGTGCTGT TGATCTGGTC GTGCACTCTC TGGATGATTC GCAACAGGAG GAAGTACGCC
    ATGATCAGCT CTCCGTTCAT GGTTGTCTAT GCCAATCTGC TGCTGGTACT GCAGTACATA
    TGGAGCTTTG AACTTCCAGA AATCAAGAAA GTCCCTGGAT TTCTAGAAAA GAAAGAGCCT
    GGAGAGCTTG CCTCAAAGAT CCTCTTCACC ATCACATTTT GGCTGCTCCT GAGGCAGCAT
    CTCACAGAGC AGAAGGCCCT TCAGGAAAAG GAAGCACTTC TGTCTGAAGT CAAAATTGGA
    AGTCAAGAAC TTGACGAAAA GGATGAGGAA CTCCAAGATA TACAAGTGGA AGGAGAGCCC
    ATAGAGAAGG AAGAAGAGGA GGAGGAAATA AAGGAAGACA GACATGAGGT AAAGAAGGAA
    GAGGAAGAGG AAGAGGAAGA TGACGATGAC CAGGACATCA TGAAGGTTCT GGGCAATCTG
    GTGGTGGCCC TCTTCATCAA GTATTGGATC TACGTCTGCG GGGGCATGTT CTTTTTTGTC
    AGCTTTGAGG GGAAAATCGT CATGTACAAA ATCATCTACA TGGTGCTGTT CCTGTTCTGT
    GTGGCCTTGT ACCAGGTGCA CTACGAGTGG TGGAGGAAGA TCCTGAAATA CTTCTGGATG
    TCGGTGGTCA TTTACACCAT GCTGGTGCTC ATCTTCATCT ACACTTACCA GTTTGAGAAC
    TTCCCAGGCC TGTGGCAAAA CATGACCGGG TTAAAGAAAG AGAAGCTTGA GGATCTGGGC
    CTGAAGCAGT TCACTGTGGC CGAACTGTTC ACACGAATAT TCATCCCCAC CTCCTTTCTG
    CTGGTCTGCA TCCTGCACCT CCACTATTTC CATGACCGGT TCCTGGAACT CACAGACCTC
    AAGTCCATCC CCAGCAAAGA AGACAACACC ATCTACAGGT TAGCCCACCC TGAAGGAAGC
    CTCCCAGACC TAGCCATTAT GAACATGACG GCCAGCCTCG ATAAGCCTGA GAGGCAGAAG
    TTGGCAGAGC CTGGGGAGGA GAGGACAGAA GAATGTGCCA AGAAGGCAGA GAAGGGTGAG
    GCTGGGAAAG ACAGTGATGA GTCTGAGGAG GAGGAAGGTG AGGAAGAGGA ATCCGAGGAG
    GAAGAATCCT CAGATCTCAG GAACAAGTGG CACCTCGTGA TTGACCGGCT CACTGTGCTC
    TTTCTAAAGT TCCTGGAGTA TTTCCACAAG TTGCAGGTGT TTATGTGGTG GATCTTGGAG
    CTGCACATCA TCAAAATCGT CTCATCGTAC ATTATCTGGG TGACTGTCAA AGAGGTGTCT
    CTGTTCAACT ATGTATTTTT GATTTCTTGG GCATTTGCTC TGCCATATGC CAAACTCCGC
    CGTGTGGCTT CCAGTGTCTG CACTGTCTGG ACGTGTGTGA TCATCGTCTG CAAAATGTTG
    TACCAGCTGC AAACCATTAA GCCTGAGAAC TTTTCTGTCA ACTGCTCCTT GCCCAATGAA
    AATCAAACCA ACATACCCCT TAATGAGTTG AACAAGTCTC TTCTCTACAG TGCCCCTGTT
    GATCCCACTG AGTGGGTTGG CCTGAGGAAG TCCTCCCCTT TACTCGTCTA CCTGAGGAAC
    AACCTCCTGA TGTTGGCGAT TTTGGCCTTC GAAGTCACTG TTTACCGACA TCAGGAGTAC
    TATCGAGGCC GAAACAACTT GACTGCTCCG GTGTCTAAAA CTATCTTTCA TGACATCACA
    AGGTTACATC TAGATGATGG GCTTATCAAT TGTGCCAAAT ATTTTGTAAA CTACTTCTTC
    TACAAGTTTG GCCTAGAGAC CTGTTTCCTA ATGTCAGTGA ACGTAATTGG ACAGCGAATG
    GACTTCTACG CCATGATTCA TGCCTGTTGG CTGATCGGTG TCTTGTACCG TCGCAGAAGA
    AAAGCCATTG CTGAGGTCTG GCCCAAGTAC TGCTGCTTCC TGGCTTGCAT CATCACCTTC
    CAGTACTTTG TCTGCATAGG CATCCCTCCT GCCCCCTGCC GAGATTACCC ATGGAGGTTC
    AAGGGTGCCA ACTTCAATGA CAACATCATC AAGTGGCTGT ACTTCCCAGA TTTCATCGTG
    CGGCCCAACC CTGTGTTTCT TGTCTATGAC TTCATGCTGC TCCTGTGTGC TTCCTTACAA
    CGACAGATTT TTGAGGACGA AAACAAGGCA GCTGTGCGCA TCATGGCCGG GGACAATGTC
    GAGATTTGCA TGAACCTTGA CGCGGCCTCA TTCAGCCAGC ATAACCCTGT ACCAGATTTC
    ATTCACTGCA GATCTTACTT GGACATGTCC AAAGTGATCA TCTTCAGCTA CCTCTTCTGG
    TTCGTCCTCA CCATCATCTT CATTACCGGA ACGACCAGGA TCAGCATCTT TTGCATGGGT
    TACCTGGTGG CCTGTTTCTA TTTCCTGCTC TTTGGAGGTG ATCTGCTCCT GAAACCCATC
    AAGAGCATCC TACGCTACTG GGACTGGCTG ATTGCATACA ACGTGTTTGT GATCACCATG
    AAAAACATAC TGTCAATAGG AGCCTGTGGA TACATCGGTG CACTGGTGAG AAACAGCTGC
    TGGCTGATCC AAGCGTTCAG CCTGGCCTGC ACGGTGAAAG GCTATAAAAT GCCTGAAGAT
    GACTCCAGAT GCAAGCTGCC CAGTGGGGAG GCAGGGATCA TCTGGGACAG CATCTGCTTT
    GCGTTTCTCC TGCTACAGAG GAGAGTCTTC ATGAGTTACT ATTTCCTGCA TGTCGTGGCT
    GACATTAAAG CATCCCAGAT CCTGGCATCC AGAGGGGCCG AACTTTTCCA GGCTACAATT
    GTGAAAGCTG TAAAGGCAAG AATTGAAGAA GAGAAAAAAT CCATGGACCA GCTGAAGAGA
    CAGATGGATC GCATCAAAGC CCGGCAGCAA AAGTACAAAA AGGGTAAGGA GAGGATGCTG
    AGCTTGACCC AGGAGTCAGG GGAAGGCCAG GACATCCAGA AAGTCTCAGA AGAAGACGAT
    GAAAGAGAAG CAGACAAACA GAAAGCCAAG GGCAAAAAAA AGCAGTGGTG GCGGCCTTGG
    GTTGATCATG CTTCCATGGT CAGGAGTGGA GATTATTATT TGTTTGAAAC GGATAGTGAA
    GAGGAGGAAG AAGAGGAACT CAAAAAGGAG GATGAAGAAC CACCACGGAA GTCTGCATTC
    CAGAGAGCTA TTGGGAAGTT TGCCTCAGCC ATTTTAGCCT TGCCAAAGTC TGTCATTAAA
    CTTCCCAAAA CTATTCTTCA GTATTTAATC AGAGCTGCAA AGTTCGTCTA CCAAGCCTGG
    ATTACCGATC CTAAAACAGC ACTCCGACAG AGGAGGAAAG AGAAGAAGAA AATGGCCAGA
    GAAGAGCAGA AGGGAAGACG CAAAGGATCT GGGGAGGGTC CAGTGGAATG GGAAGACAGA
    GAAGATGAGC CAATCAAAAA GAAATCTGAT GGACCAGATA ATATCATCAA GAGGATATTT
    AATATCCTGA AGTTCACCTG GGTCCTGTTC CTGGCCACAG TGGACAGTTT CACTACTTGG
    CTCAACTCCA TCTCAAGAGA GCATATCGAC ATATCCACAG TTCTGCGGAT TGAAAGATGC
    ATGCTGACCC GAGAGATAAA GAAGGGCAAC GTTCCGACAC GGGAAAGCAT CCACATGTAC
    TACCAGAACC ACATGATGAA CCTTTCCAGA GAATCCGGAC TGGACACAAT CGACGAGCAC
    TCTGGCGCTG GTTCCAGGGC CCAGGCAGCA CACAGGATGG ATAGCTTAGA TTCCCATGAC
    AGTATCTCCA GCTGTTACAC TGAAGCAACC CTGCTGATCT CAAGGCAGTC CACCCTTGAT
    GATTTAGATG GACAAGACCC TGTTCCTAAA ACAAGCGAGC GTGCTCGGCC TAGGCTCCGT
    AAAGTGTTCA GCATGGACAT GTCCTCCTCA TCAGCTGACA GTGGCAGTGT AGCATCAAGC
    GAGCCCACAC AGTGCACCAT GCTGTATTCA CGCCAAGGAA CTACGGAAAC CATTGAGGAG
    GTAGAGGCTG AAGCTGAGGA GGAGGTGGTA GAGAGTCTGG AGCCTGAGCT CCATGATGCC
    GAAGAGAAGG AATATGCAGC TGACTATGAA GCAGGGGTAG AGGAGGTCAG CCTCACTCCA
    GATGAGGAGT TGCCTCAGTT CTCAACAGAT GACGGTGACG TGGAGGCCCC GCCCTCCTAC
    AGCAAGGCTG TCAGCTTTGA GCACCTCTCG TTTGCATCAC AAGATGACTC CGGGGGCAAG
    AACCACATGG TGGTCAGCCC TGACGACAGC CGCACAGACA AGCTGGAGTC CAGTATCTTA
    CCGCCCCTGA CTCACGAGCT GACAGCCAGC GATCTGCTCA TGAGCAAGAT GTTCCGTGAT
    GATGAGCTTG AAGAATCTGA GAAATTCTAC GTGGACCAGC CCCGGTTCTT GCTGCTATTC
    TATGCCATGT ATAACACTCT GGTAGCCCGC TCGGAAATGG TGTGCTATTT CGTGATCATC
    CTGAACCACA TGATCTCTGC CTCCATCATT ACTCTTCTGT TGCCCATCCT GATCTTCCTC
    TGGGCCATGC TGTCCGTCCC CAGGCCCAGC CGGCGCTTCT GGATGATGGC CATCGTCTAC
    ACTGAGGTGG CGATTGTAGT GAAATATTTC TTCCAGTTTG GCTTCTTTCC TTGGAATAAG
    GACTTGGAAA TTTACAAGGA CAGGCCCTAC TTTCCACCAA ACATCATAGG GGTGGAAAAG
    AAAGAGGGCT ATGTTCTTTA CGACCTCATT CAGCTGCTGG CTCTGTTCTT CCATCGATCC
    ATTCTGAAGT GTCATGGCCT ATGGGACGAG GATGACATTG TTGACAGTAA CACAGACAAG
    GAAGGATCTG ATGATGAGCT GTCCCTCGGT CAGGGCAGAA GAGGCTCCTC AGAGTCACTG
    AAGTCCATCA ACTTGGCTGC CTCTGTAGAG TCCGTCCATG TGACCTTCCC AGAGCAGCCA
    GCAGCCATCC GGAGAAAACG CTCCTGCAGC AGCTCGCAGA TCTCCCCACG GTCCAGCTTC
    TCTTCAAATA GGTCCAAAAG AGGCAGCACA AGCACCCGAA ACAGCAGCCA AAAAGGAAGC
    AGTGTTTTGA GCATTAAACA AAAAAGCAAA AGAGAACTTT ACATGGAAAA GCTTCAAGAA
    CATTTAATCA AAGCAAAGGC CTTTACCATA AAGAAAACTC TACAAATCTA TGTACCCATT
    CGGCAATTCT TCTATGACCT CATCCACCCA GACTACAGTG CTGTGACTGA CGTGTATGTG
    CTGATGTTCC TGGCCGACAC CGTGGACTTC ATCATCATCG TCTTTGGCTT CTGGGCTTTT
    GGGAAACACT CGGCAGCTGC AGATATCACC TCTTCCCTAT CAGAAGACCA GGTCCCCGGG
    CCATTCCTGG TGATGGTTCT CATTCAGTTT GGAACCATGG TGGTGGACCG TGCACTCTAC
    CTCAGGAAGA CTGTGCTTGG CAAGGTCATT TTCCAGGTCA TCCTCGTGTT TGGAATTCAC
    TTCTGGATGT TCTTCATCCT ACCCAGTGTG ACTGAGAGGA AATTCAGCCA GAACCTGGTG
    GCACAGCTCT GGTACTTTGT GAAATGTGTG TACTTTGGGT TGTCTGCCTA CCAAATCCGC
    TGTGGCTACC CAACACGTGT GCTCGGAAAC TTCCTCACGA AGAGCTACAA TTATGTCAAC
    CTCTTCTTGT TTCAAGGGTT TCGTCTCGTC CCATTTCTGA CAGAGCTGCG GGCTGTGATG
    GACTGGGTGT GGACAGATAC AACCTTGAGC CTCTCCAGCT GGATTTGTGT GGAGGACATT
    TACGCACACA TTTTCATCTT AAAGTGCTGG CGAGAGTCGG AAAAGAGATA CCCTCAACCC
    AGGGGCCAGA AGAAGAAGAA AGCTGTGAAG TATGGCATGG GCGGAATGAT CATTGTCCTG
    CTCATCTGTA TCGTCTGGTT TCCTCTGCTC TTCATGTCTT TGATCAAGTC TGTCGCAGGG
    GTTATTAACC AACCCCTGGA TGTGTCCGTC ACAATAACCC TGGGAGGCTA TCAGCCTATC
    TTCACAATGA GTGCCCAGCA AAGCCAGCTG AAAGTTATGG ATGGTACAAA GTATAACGAA
    TTCATAAAGT CTTTCGGCCC CAACAGTGGC GCTATGCAAT TTCTGGAGAA TTATGAAAGG
    CAAGACATAA CAGTAGCGGA ACTGGAAGGG AATTCGAATT CTTTGTGGAC CATCAGCCCT
    CCCAGTAAGC AGAAAATGAT ACAGGAGCTC ACCGACCCCA ATAGTTGCTT CTCTGTTGTT
    TTTTCATGGA GTATCCAGAG AAACATGACT CTGGGTGCAA AGGCTGAAAT AGCAACGGAC
    AAGCTTTCTT TTCCTCTCAC GGATGCGACA CGAAGTAGCA TAGCCAAAAT GATTGCTGGC
    AATGACACAG AAAGTTCCAA CACACCAGTG ACAATAGAAA AGATCTACCC ATACTATGTG
    AAGGCACCCA GTGACTCAAA CTCAAAGCCT ATAAAACAGC TTCTGTCAGA AAACAATTTC
    ATGAATATCA CCATCATTTT GTTCAGAGAC AATGTCACAA AGTCCAATAG TGAGTGGTGG
    GTTCTCAACC TGACTGGAAG TAGGATATTC AATCAGGGAT CCCAAGCCTT GGAGCTGGTG
    GTCTTCAATG ACAAAGTCAG CCCCCCAAGT CTGGGCTTCT TGGCTGGCTA TGGCATCATG
    GGATTGTATG CATCCGTTGT CCTTGTAATT GGGAAGTTTG TTCGCGAATT CTTCAGTGGG
    ATTTCTCACT CTATCATGTT TGAAGAGCTT CCAAATGTGG ACAGAATTTT GAAGTTATGC
    ACAGATATAT TTCTCGTACG GGAGACAGGG GAACTGGAGC TGGAGGAAGA CCTCTATGCC
    AAATTGATAT TCCTGTACAG GTCGCCAGAA ACCATGATCA AGTGGACTAG AGAAAAAACA
    AATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017443368.1
    CDS940..9465
    Translation

    Target ORF information:

    RefSeq Version XM_017587879.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017587879.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTCCCCAT CTGCCTGGCA 
    GTAGCATGTG CATTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCG ACGATGCAAG GACACACAGG GCGGCTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCCTTT CTGCTCCTTC ACATCATTTT CCACATCACA
    CTGGCCAGCC TGGAAGCTCA GCACCGCATC GCACCCGCTT ACAACTGCTC AACTTGGGAA
    AAGACCTTCC GGCAGATCGG CTTCGAAAGC TTGAAGGGAG CTGATGCTGG CAATGGCATC
    AGGGTGTTTG TGCCTGATAT CGGGATGTTC ATTGCGAGCC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACGGAA GAAGTAGCCC AATTTAATTC CGAGTGCGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GACTCAGAAG AAGCGCTGAT TTATGAGGAG
    GACTTAGATG GGGAAGAAGG CATGGAAGGC GAGCTGGAAG AAAGCACAAA GTTAAAAATG
    TTTCGCAGGC TCGCCTCCGT CGCTTCCAAG CTTAAGGAGT TCATCGGCAA TATGATAACC
    ACGGCGGGCA AGGTGGTGGT GACCATCCTG TTGGGCTCCT CTGGCATGAT GCTGCCATCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT CTGGGTCTGT GCACCTGGTG GTCCTGGTGC
    CGAACTTTTG ACCCATTGCT GTTTGGCTGC CTCTGTGTCC TGTTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTTCAGTTCT TCCAAGAAGC AGTCCCACCC
    AATGACTACT ATGCAAGGTT GTTTGGAATC AAATCCGTTA TTCAAACAGA CTGCTCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTACAC GCTGGCCACG CTGATCCGCA TTTGGTTGCA AGAGCCTCTT
    GTGCAGGAGG AGATGTCGAA GGAAGAGGAG GGTGCACTGG ATGGCTGCTC CAATCAGAAC
    ACAGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    CTTTTATCCA TGACCCAAGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACTGTGAAC
    GGCAACCCTG TGGACTACCA CACCATCCAC CCAAGTCTGC CCATAGAGAA TGGCCCCGCC
    AAGACGGACT TGTATACTAC TCCTCAGTAC CGGTGGGAGC CCTCAGAGGA GTCCTCAGAA
    AAGAAAGAGG AGGAAGAGGA CAAGAGAGAA GACTCAGAAG GGGAAGGGAG CCAAGAGGAA
    AAACGGAGTG TGAGAATTCA TGCCATGGTC GCCGTGTTCC AGTTCATCAT GAAGCAGAGT
    TACATCTGTG CCCTCATCGC CATGATGGCA TGGAGCATCA CCTACCACAG CTGGCTGACA
    TTTGTGCTGT TGATCTGGTC GTGCACTCTC TGGATGATTC GCAACAGGAG GAAGTACGCC
    ATGATCAGCT CTCCGTTCAT GGTTGTCTAT GCCAATCTGC TGCTGGTACT GCAGTACATA
    TGGAGCTTTG AACTTCCAGA AATCAAGAAA GTCCCTGGAT TTCTAGAAAA GAAAGAGCCT
    GGAGAGCTTG CCTCAAAGAT CCTCTTCACC ATCACATTTT GGCTGCTCCT GAGGCAGCAT
    CTCACAGAGC AGAAGGCCCT TCAGGAAAAG GAAGCACTTC TGTCTGAAGT CAAAATTGGA
    AGTCAAGAAC TTGACGAAAA GGATGAGGAA CTCCAAGATA TACAAGTGGA AGGAGAGCCC
    ATAGAGAAGG AAGAAGAGGA GGAGGAAATA AAGGAAGACA GACATGAGGT AAAGAAGGAA
    GAGGAAGAGG AAGAGGAAGA TGACGATGAC CAGGACATCA TGAAGGTTCT GGGCAATCTG
    GTGGTGGCCC TCTTCATCAA GTATTGGATC TACGTCTGCG GGGGCATGTT CTTTTTTGTC
    AGCTTTGAGG GGAAAATCGT CATGTACAAA ATCATCTACA TGGTGCTGTT CCTGTTCTGT
    GTGGCCTTGT ACCAGGTGCA CTACGAGTGG TGGAGGAAGA TCCTGAAATA CTTCTGGATG
    TCGGTGGTCA TTTACACCAT GCTGGTGCTC ATCTTCATCT ACACTTACCA GTTTGAGAAC
    TTCCCAGGCC TGTGGCAAAA CATGACCGGG TTAAAGAAAG AGAAGCTTGA GGATCTGGGC
    CTGAAGCAGT TCACTGTGGC CGAACTGTTC ACACGAATAT TCATCCCCAC CTCCTTTCTG
    CTGGTCTGCA TCCTGCACCT CCACTATTTC CATGACCGGT TCCTGGAACT CACAGACCTC
    AAGTCCATCC CCAGCAAAGA AGACAACACC ATCTACAGGT TAGCCCACCC TGAAGGAAGC
    CTCCCAGACC TAGCCATTAT GAACATGACG GCCAGCCTCG ATAAGCCTGA GAGGCAGAAG
    TTGGCAGAGC CTGGGGAGGA GAGGACAGAA GAATGTGCCA AGAAGGCAGA GAAGGGTGAG
    GCTGGGAAAG ACAGTGATGA GTCTGAGGAG GAGGAAGGTG AGGAAGAGGA ATCCGAGGAG
    GAAGAATCCT CAGATCTCAG GAACAAGTGG CACCTCGTGA TTGACCGGCT CACTGTGCTC
    TTTCTAAAGT TCCTGGAGTA TTTCCACAAG TTGCAGGTGT TTATGTGGTG GATCTTGGAG
    CTGCACATCA TCAAAATCGT CTCATCGTAC ATTATCTGGG TGACTGTCAA AGAGGTGTCT
    CTGTTCAACT ATGTATTTTT GATTTCTTGG GCATTTGCTC TGCCATATGC CAAACTCCGC
    CGTGTGGCTT CCAGTGTCTG CACTGTCTGG ACGTGTGTGA TCATCGTCTG CAAAATGTTG
    TACCAGCTGC AAACCATTAA GCCTGAGAAC TTTTCTGTCA ACTGCTCCTT GCCCAATGAA
    AATCAAACCA ACATACCCCT TAATGAGTTG AACAAGTCTC TTCTCTACAG TGCCCCTGTT
    GATCCCACTG AGTGGGTTGG CCTGAGGAAG TCCTCCCCTT TACTCGTCTA CCTGAGGAAC
    AACCTCCTGA TGTTGGCGAT TTTGGCCTTC GAAGTCACTG TTTACCGACA TCAGGAGTAC
    TATCGAGGCC GAAACAACTT GACTGCTCCG GTGTCTAAAA CTATCTTTCA TGACATCACA
    AGGTTACATC TAGATGATGG GCTTATCAAT TGTGCCAAAT ATTTTGTAAA CTACTTCTTC
    TACAAGTTTG GCCTAGAGAC CTGTTTCCTA ATGTCAGTGA ACGTAATTGG ACAGCGAATG
    GACTTCTACG CCATGATTCA TGCCTGTTGG CTGATCGGTG TCTTGTACCG TCGCAGAAGA
    AAAGCCATTG CTGAGGTCTG GCCCAAGTAC TGCTGCTTCC TGGCTTGCAT CATCACCTTC
    CAGTACTTTG TCTGCATAGG CATCCCTCCT GCCCCCTGCC GAGATTACCC ATGGAGGTTC
    AAGGGTGCCA ACTTCAATGA CAACATCATC AAGTGGCTGT ACTTCCCAGA TTTCATCGTG
    CGGCCCAACC CTGTGTTTCT TGTCTATGAC TTCATGCTGC TCCTGTGTGC TTCCTTACAA
    CGACAGATTT TTGAGGACGA AAACAAGGCA GCTGTGCGCA TCATGGCCGG GGACAATGTC
    GAGATTTGCA TGAACCTTGA CGCGGCCTCA TTCAGCCAGC ATAACCCTGT ACCAGATTTC
    ATTCACTGCA GATCTTACTT GGACATGTCC AAAGTGATCA TCTTCAGCTA CCTCTTCTGG
    TTCGTCCTCA CCATCATCTT CATTACCGGA ACGACCAGGA TCAGCATCTT TTGCATGGGT
    TACCTGGTGG CCTGTTTCTA TTTCCTGCTC TTTGGAGGTG ATCTGCTCCT GAAACCCATC
    AAGAGCATCC TACGCTACTG GGACTGGCTG ATTGCATACA ACGTGTTTGT GATCACCATG
    AAAAACATAC TGTCAATAGG AGCCTGTGGA TACATCGGTG CACTGGTGAG AAACAGCTGC
    TGGCTGATCC AAGCGTTCAG CCTGGCCTGC ACGGTGAAAG GCTATAAAAT GCCTGAAGAT
    GACTCCAGAT GCAAGCTGCC CAGTGGGGAG GCAGGGATCA TCTGGGACAG CATCTGCTTT
    GCGTTTCTCC TGCTACAGAG GAGAGTCTTC ATGAGTTACT ATTTCCTGCA TGTCGTGGCT
    GACATTAAAG CATCCCAGAT CCTGGCATCC AGAGGGGCCG AACTTTTCCA GGCTACAATT
    GTGAAAGCTG TAAAGGCAAG AATTGAAGAA GAGAAAAAAT CCATGGACCA GCTGAAGAGA
    CAGATGGATC GCATCAAAGC CCGGCAGCAA AAGTACAAAA AGGGTAAGGA GAGGATGCTG
    AGCTTGACCC AGGAGTCAGG GGAAGGCCAG GACATCCAGA AAGTCTCAGA AGAAGACGAT
    GAAAGAGAAG CAGACAAACA GAAAGCCAAG GGCAAAAAAA AGCAGTGGTG GCGGCCTTGG
    GTTGATCATG CTTCCATGGT CAGGAGTGGA GATTATTATT TGTTTGAAAC GGATAGTGAA
    GAGGAGGAAG AAGAGGAACT CAAAAAGGAG GATGAAGAAC CACCACGGAA GTCTGCATTC
    CAGAGAGCTA TTGGGAAGTT TGCCTCAGCC ATTTTAGCCT TGCCAAAGTC TGTCATTAAA
    CTTCCCAAAA CTATTCTTCA GTATTTAATC AGAGCTGCAA AGTTCGTCTA CCAAGCCTGG
    ATTACCGATC CTAAAACAGC ACTCCGACAG AGGAGGAAAG AGAAGAAGAA AATGGCCAGA
    GAAGAGCAGA AGGGAAGACG CAAAGGATCT GGGGAGGGTC CAGTGGAATG GGAAGACAGA
    GAAGATGAGC CAATCAAAAA GAAATCTGAT GGACCAGATA ATATCATCAA GAGGATATTT
    AATATCCTGA AGTTCACCTG GGTCCTGTTC CTGGCCACAG TGGACAGTTT CACTACTTGG
    CTCAACTCCA TCTCAAGAGA GCATATCGAC ATATCCACAG TTCTGCGGAT TGAAAGATGC
    ATGCTGACCC GAGAGATAAA GAAGGGCAAC GTTCCGACAC GGGAAAGCAT CCACATGTAC
    TACCAGAACC ACATGATGAA CCTTTCCAGA GAATCCGGAC TGGACACAAT CGACGAGCAC
    TCTGGCGCTG GTTCCAGGGC CCAGGCAGCA CACAGGATGG ATAGCTTAGA TTCCCATGAC
    AGTATCTCCA GCTGTTACAC TGAAGCAACC CTGCTGATCT CAAGGCAGTC CACCCTTGAT
    GATTTAGATG GACAAGACCC TGTTCCTAAA ACAAGCGAGC GTGCTCGGCC TAGGCTCCGT
    AAAGTGTTCA GCATGGACAT GTCCTCCTCA TCAGCTGACA GTGGCAGTGT AGCATCAAGC
    GAGCCCACAC AGTGCACCAT GCTGTATTCA CGCCAAGGAA CTACGGAAAC CATTGAGGAG
    GTAGAGGCTG AAGCTGAGGA GGAGGTGGTA GAGAGTCTGG AGCCTGAGCT CCATGATGCC
    GAAGAGAAGG AATATGCAGC TGACTATGAA GCAGGGGTAG AGGAGGTCAG CCTCACTCCA
    GATGAGGAGT TGCCTCAGTT CTCAACAGAT GACGGTGACG TGGAGGCCCC GCCCTCCTAC
    AGCAAGGCTG TCAGCTTTGA GCACCTCTCG TTTGCATCAC AAGATGACTC CGGGGGCAAG
    AACCACATGG TGGTCAGCCC TGACGACAGC CGCACAGACA AGCTGGAGTC CAGTATCTTA
    CCGCCCCTGA CTCACGAGCT GACAGCCAGC GATCTGCTCA TGAGCAAGAT GTTCCGTGAT
    GATGAGCTTG AAGAATCTGA GAAATTCTAC GTGGACCAGC CCCGGTTCTT GCTGCTATTC
    TATGCCATGT ATAACACTCT GGTAGCCCGC TCGGAAATGG TGTGCTATTT CGTGATCATC
    CTGAACCACA TGATCTCTGC CTCCATCATT ACTCTTCTGT TGCCCATCCT GATCTTCCTC
    TGGGCCATGC TGTCCGTCCC CAGGCCCAGC CGGCGCTTCT GGATGATGGC CATCGTCTAC
    ACTGAGGTGG CGATTGTAGT GAAATATTTC TTCCAGTTTG GCTTCTTTCC TTGGAATAAG
    GACTTGGAAA TTTACAAGGA CAGGCCCTAC TTTCCACCAA ACATCATAGG GGTGGAAAAG
    AAAGAGGGCT ATGTTCTTTA CGACCTCATT CAGCTGCTGG CTCTGTTCTT CCATCGATCC
    ATTCTGAAGT GTCATGGCCT ATGGGACGAG GATGACATTG TTGACAGTAA CACAGACAAG
    GAAGGATCTG ATGATGAGCT GTCCCTCGGT CAGGGCAGAA GAGGCTCCTC AGAGTCACTG
    AAGTCCATCA ACTTGGCTGC CTCTGTAGAG TCCGTCCATG TGACCTTCCC AGAGCAGCCA
    GCAGCCATCC GGAGAAAACG CTCCTGCAGC AGCTCGCAGA TCTCCCCACG GTCCAGCTTC
    TCTTCAAATA GGTCCAAAAG AGGCAGCACA AGCACCCGAA ACAGCAGCCA AAAAGGAAGC
    AGTGTTTTGA GCATTAAACA AAAAAGCAAA AGAGAACTTT ACATGGAAAA GCTTCAAGAA
    CATTTAATCA AAGCAAAGGC CTTTACCATA AAGAAAACTC TACAAATCTA TGTACCCATT
    CGGCAATTCT TCTATGACCT CATCCACCCA GACTACAGTG CTGTGACTGA CGTGTATGTG
    CTGATGTTCC TGGCCGACAC CGTGGACTTC ATCATCATCG TCTTTGGCTT CTGGGCTTTT
    GGGAAACACT CGGCAGCTGC AGATATCACC TCTTCCCTAT CAGAAGACCA GGTCCCCGGG
    CCATTCCTGG TGATGGTTCT CATTCAGTTT GGAACCATGG TGGTGGACCG TGCACTCTAC
    CTCAGGAAGA CTGTGCTTGG CAAGGTCATT TTCCAGGTCA TCCTCGTGTT TGGAATTCAC
    TTCTGGATGT TCTTCATCCT ACCCAGTGTG ACTGAGAGGA AATTCAGCCA GAACCTGGTG
    GCACAGCTCT GGTACTTTGT GAAATGTGTG TACTTTGGGT TGTCTGCCTA CCAAATCCGC
    TGTGGCTACC CAACACGTGT GCTCGGAAAC TTCCTCACGA AGAGCTACAA TTATGTCAAC
    CTCTTCTTGT TTCAAGGGTT TCGTCTCGTC CCATTTCTGA CAGAGCTGCG GGCTGTGATG
    GACTGGGTGT GGACAGATAC AACCTTGAGC CTCTCCAGCT GGATTTGTGT GGAGGACATT
    TACGCACACA TTTTCATCTT AAAGTGCTGG CGAGAGTCGG AAAAGAGATA CCCTCAACCC
    AGGGGCCAGA AGAAGAAGAA AGCTGTGAAG TATGGCATGG GCGGAATGAT CATTGTCCTG
    CTCATCTGTA TCGTCTGGTT TCCTCTGCTC TTCATGTCTT TGATCAAGTC TGTCGCAGGG
    GTTATTAACC AACCCCTGGA TGTGTCCGTC ACAATAACCC TGGGAGGCTA TCAGCCTATC
    TTCACAATGA GTGCCCAGCA AAGCCAGCTG AAAGTTATGG ATGGTACAAA GTATAACGAA
    TTCATAAAGT CTTTCGGCCC CAACAGTGGC GCTATGCAAT TTCTGGAGAA TTATGAAAGG
    CAAGACATAA CAGTAGCGGA ACTGGAAGGG AATTCGAATT CTTTGTGGAC CATCAGCCCT
    CCCAGTAAGC AGAAAATGAT ACAGGAGCTC ACCGACCCCA ATAGTTGCTT CTCTGTTGTT
    TTTTCATGGA GTATCCAGAG AAACATGACT CTGGGTGCAA AGGCTGAAAT AGCAACGGAC
    AAGCTTTCTT TTCCTCTCAC GGATGCGACA CGAAGTAGCA TAGCCAAAAT GATTGCTGGC
    AATGACACAG AAAGTTCCAA CACACCAGTG ACAATAGAAA AGATCTACCC ATACTATGTG
    AAGGCACCCA GTGACTCAAA CTCAAAGCCT ATAAAACAGC TTCTGTCAGA AAACAATTTC
    ATGAATATCA CCATCATTTT GTTCAGAGAC AATGTCACAA AGTCCAATAG TGAGTGGTGG
    GTTCTCAACC TGACTGGAAG TAGGATATTC AATCAGGGAT CCCAAGCCTT GGAGCTGGTG
    GTCTTCAATG ACAAAGTCAG CCCCCCAAGT CTGGGCTTCT TGGCTGGCTA TGGCATCATG
    GGATTGTATG CATCCGTTGT CCTTGTAATT GGGAAGTTTG TTCGCGAATT CTTCAGTGGG
    ATTTCTCACT CTATCATGTT TGAAGAGCTT CCAAATGTGG ACAGAATTTT GAAGTTATGC
    ACAGATATAT TTCTCGTACG GGAGACAGGG GAACTGGAGC TGGAGGAAGA CCTCTATGCC
    AAATTGATAT TCCTGTACAG GTCGCCAGAA ACCATGATCA AGTGGACTAG AGAAAAAACA
    AATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa54721
    Clone ID Related Accession (Same CDS sequence) XM_017601119.1 , XM_017587880.1
    Accession Version XM_017601119.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8502bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product piezo-type mechanosensitive ion channel component 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005117.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTCCCCAT CTGCCTGGCA 
    GTAGCATGTG CATTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCG ACGATGCAAG GACACACAGG GCGGCTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCCTTT CTGCTCCTTC ACATCATTTT CCACATCACA
    CTGGCCAGCC TGGAAGCTCA GCACCGCATC GCACCCGCTT ACAACTGCTC AACTTGGGAA
    AAGACCTTCC GGCAGATCGG CTTCGAAAGC TTGAAGGGAG CTGATGCTGG CAATGGCATC
    AGGGTGTTTG TGCCTGATAT CGGGATGTTC ATTGCGAGCC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACGGAA GAAGTAGCCC AATTTAATTC CGAGTGCGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GACTCAGAAG AAGCGCTGAT TTATGAGGAG
    GACTTAGATG GGGAAGAAGG CATGGAAGGC GAGCTGGAAG AAAGCACAAA GTTAAAAATG
    TTTCGCAGGC TCGCCTCCGT CGCTTCCAAG CTTAAGGAGT TCATCGGCAA TATGATAACC
    ACGGCGGGCA AGGTGGTGGT GACCATCCTG TTGGGCTCCT CTGGCATGAT GCTGCCATCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT CTGGGTCTGT GCACCTGGTG GTCCTGGTGC
    CGAACTTTTG ACCCATTGCT GTTTGGCTGC CTCTGTGTCC TGTTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTTCAGTTCT TCCAAGAAGC AGTCCCACCC
    AATGACTACT ATGCAAGGTT GTTTGGAATC AAATCCGTTA TTCAAACAGA CTGCTCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTACAC GCTGGCCACG CTGATCCGCA TTTGGTTGCA AGAGCCTCTT
    GTGCAGGAGG AGATGTCGAA GGAAGAGGAG GGTGCACTGG ATGGCTGCTC CAATCAGAAC
    ACAGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    CTTTTATCCA TGACCCAAGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACTGTGAAC
    GGCAACCCTG TGGACTACCA CACCATCCAC CCAAGTCTGC CCATAGAGAA TGGCCCCGCC
    AAGACGGACT TGTATACTAC TCCTCAGTAC CGGTGGGAGC CCTCAGAGGA GTCCTCAGAA
    AAGAAAGAGG AGGAAGAGGA CAAGAGAGAA GACTCAGAAG GGGAAGGGAG CCAAGAGGAA
    AAACGGAGTG TGAGAATTCA TGCCATGGTC GCCGTGTTCC AGTTCATCAT GAAGCAGAGT
    TACATCTGTG CCCTCATCGC CATGATGGCA TGGAGCATCA CCTACCACAG CTGGCTGACA
    TTTGTGCTGT TGATCTGGTC GTGCACTCTC TGGATGATTC GCAACAGGAG GAAGTACGCC
    ATGATCAGCT CTCCGTTCAT GGTTGTCTAT GCCAATCTGC TGCTGGTACT GCAGTACATA
    TGGAGCTTTG AACTTCCAGA AATCAAGAAA GTCCCTGGAT TTCTAGAAAA GAAAGAGCCT
    GGAGAGCTTG CCTCAAAGAT CCTCTTCACC ATCACATTTT GGCTGCTCCT GAGGCAGCAT
    CTCACAGAGC AGAAGGCCCT TCAGGAAAAG GAAGCACTTC TGTCTGAAGT CAAAATTGGA
    AGTCAAGAAC TTGACGAAAA GGATGAGGAA CTCCAAGATA TACAAGTGGA AGGAGAGCCC
    ATAGAGAAGG AAGAAGAGGA GGAGGAAATA AAGGAAGACA GACATGAGGT AAAGAAGGAA
    GAGGAAGAGG AAGAGGAAGA TGACGATGAC CAGGACATCA TGAAGGTTCT GGGCAATCTG
    GTGGTGGCCC TCTTCATCAA GTATTGGATC TACGTCTGCG GGGGCATGTT CTTTTTTGTC
    AGCTTTGAGG GGAAAATCGT CATGTACAAA ATCATCTACA TGGTGCTGTT CCTGTTCTGT
    GTGGCCTTGT ACCAGGTGCA CTACGAGTGG TGGAGGAAGA TCCTGAAATA CTTCTGGATG
    TCGGTGGTCA TTTACACCAT GCTGGTGCTC ATCTTCATCT ACACTTACCA GTTTGAGAAC
    TTCCCAGGCC TGTGGCAAAA CATGACCGGG TTAAAGAAAG AGAAGCTTGA GGATCTGGGC
    CTGAAGCAGT TCACTGTGGC CGAACTGTTC ACACGAATAT TCATCCCCAC CTCCTTTCTG
    CTGGTCTGCA TCCTGCACCT CCACTATTTC CATGACCGGT TCCTGGAACT CACAGACCTC
    AAGTCCATCC CCAGCAAAGA AGACAACACC ATCTACAGCC ATGCCAAGGT CAATGGTCGC
    GTGTACCTAA TAATAAATAG CATCAAGAAA AAATTACCAA TCCACCAGAA TGAGTTAGCC
    CACCCTGAAG GAAGCCTCCC AGACCTAGCC ATTATGAACA TGACGGCCAG CCTCGATAAG
    CCTGAGAGGC AGAAGTTGGC AGAGCCTGGG GAGGAGAGGA CAGAAGAATG TGCCAAGAAG
    GCAGAGAAGG GTGAGGCTGG GAAAGACAGT GATGAGTCTG AGGAGGAGGA AGGTGAGGAA
    GAGGAATCCG AGGAGGAAGA ATCCTCAGAT CTCAGGAACA AGTGGCACCT CGTGATTGAC
    CGGCTCACTG TGCTCTTTCT AAAGTTCCTG GAGTATTTCC ACAAGTTGCA GGTGTTTATG
    TGGTGGATCT TGGAGCTGCA CATCATCAAA ATCGTCTCAT CGTACATTAT CTGGGTGACT
    GTCAAAGAGG TGTCTCTGTT CAACTATGTA TTTTTGATTT CTTGGGCATT TGCTCTGCCA
    TATGCCAAAC TCCGCCGTGT GGCTTCCAGT GTCTGCACTG TCTGGACGTG TGTGATCATC
    GTCTGCAAAA TGTTGTACCA GCTGCAAACC ATTAAGCCTG AGAACTTTTC TGTCAACTGC
    TCCTTGCCCA ATGAAAATCA AACCAACATA CCCCTTAATG AGTTGAACAA GTCTCTTCTC
    TACAGTGCCC CTGTTGATCC CACTGAGTGG GTTGGCCTGA GGAAGTCCTC CCCTTTACTC
    GTCTACCTGA GGAACAACCT CCTGATGTTG GCGATTTTGG CCTTCGAAGT CACTGTTTAC
    CGACATCAGG AGTACTATCG AGGCCGAAAC AACTTGACTG CTCCGGTGTC TAAAACTATC
    TTTCATGACA TCACAAGGTT ACATCTAGAT GATGGGCTTA TCAATTGTGC CAAATATTTT
    GTAAACTACT TCTTCTACAA GTTTGGCCTA GAGACCTGTT TCCTAATGTC AGTGAACGTA
    ATTGGACAGC GAATGGACTT CTACGCCATG ATTCATGCCT GTTGGCTGAT CGGTGTCTTG
    TACCGTCGCA GAAGAAAAGC CATTGCTGAG GTCTGGCCCA AGTACTGCTG CTTCCTGGCT
    TGCATCATCA CCTTCCAGTA CTTTGTCTGC ATAGGCATCC CTCCTGCCCC CTGCCGAGAT
    TACCCATGGA GGTTCAAGGG TGCCAACTTC AATGACAACA TCATCAAGTG GCTGTACTTC
    CCAGATTTCA TCGTGCGGCC CAACCCTGTG TTTCTTGTCT ATGACTTCAT GCTGCTCCTG
    TGTGCTTCCT TACAACGACA GATTTTTGAG GACGAAAACA AGGCAGCTGT GCGCATCATG
    GCCGGGGACA ATGTCGAGAT TTGCATGAAC CTTGACGCGG CCTCATTCAG CCAGCATAAC
    CCTGTACCAG ATTTCATTCA CTGCAGATCT TACTTGGACA TGTCCAAAGT GATCATCTTC
    AGCTACCTCT TCTGGTTCGT CCTCACCATC ATCTTCATTA CCGGAACGAC CAGGATCAGC
    ATCTTTTGCA TGGGTTACCT GGTGGCCTGT TTCTATTTCC TGCTCTTTGG AGGTGATCTG
    CTCCTGAAAC CCATCAAGAG CATCCTACGC TACTGGGACT GGCTGATTGC ATACAACGTG
    TTTGTGATCA CCATGAAAAA CATACTGTCA ATAGGAGCCT GTGGATACAT CGGTGCACTG
    GTGAGAAACA GCTGCTGGCT GATCCAAGCG TTCAGCCTGG CCTGCACGGT GAAAGGCTAT
    AAAATGCCTG AAGATGACTC CAGATGCAAG CTGCCCAGTG GGGAGGCAGG GATCATCTGG
    GACAGCATCT GCTTTGCGTT TCTCCTGCTA CAGAGGAGAG TCTTCATGAG TTACTATTTC
    CTGCATGTCG TGGCTGACAT TAAAGCATCC CAGATCCTGG CATCCAGAGG GGCCGAACTT
    TTCCAGGCTA CAATTGTGAA AGCTGTAAAG GCAAGAATTG AAGAAGAGAA AAAATCCATG
    GACCAGCTGA AGAGACAGAT GGATCGCATC AAAGCCCGGC AGCAAAAGTA CAAAAAGGGT
    AAGGAGAGGA TGCTGAGCTT GACCCAGGAG TCAGGGGAAG GCCAGGACAT CCAGAAAGTC
    TCAGAAGAAG ACGATGAAAG AGAAGCAGAC AAACAGAAAG CCAAGGGCAA AAAAAAGCAG
    TGGTGGCGGC CTTGGGTTGA TCATGCTTCC ATGGTCAGGA GTGGAGATTA TTATTTGTTT
    GAAACGGATA GTGAAGAGGA GGAAGAAGAG GAACTCAAAA AGGAGGATGA AGAACCACCA
    CGGAAGTCTG CATTCCAGTT CGTCTACCAA GCCTGGATTA CCGATCCTAA AACAGCACTC
    CGACAGAGGA GGAAAGAGAA GAAGAAAATG GCCAGAGAAG AGCAGAAGGG AAGACGCAAA
    GGATCTGGGG AGGGTCCAGT GGAATGGGAA GACAGAGAAG ATGAGCCAAT CAAAAAGAAA
    TCTGATGGAC CAGATAATAT CATCAAGAGG ATATTTAATA TCCTGAAGTT CACCTGGGTC
    CTGTTCCTGG CCACAGTGGA CAGTTTCACT ACTTGGCTCA ACTCCATCTC AAGAGAGCAT
    ATCGACATAT CCACAGTTCT GCGGATTGAA AGATGCATGC TGACCCGAGA GATAAAGAAG
    GGCAACGTTC CGACACGGGA AAGCATCCAC ATGTACTACC AGAACCACAT GATGAACCTT
    TCCAGAGAAT CCGGACTGGA CACAATCGAC GAGCACTCTG GCGCTGGTTC CAGGGCCCAG
    GCAGCACACA GGATGGATAG CTTAGATTCC CATGACAGTA TCTCCAGCTG TTACACTGAA
    GCAACCCTGC TGATCTCAAG GCAGTCCACC CTTGATGATT TAGATGGACA AGACCCTGTT
    CCTAAAACAA GCGAGCGTGC TCGGCCTAGG CTCCGTAAAG TGTTCAGCAT GGACATGTCC
    TCCTCATCAG CTGACAGTGG CAGTGTAGCA TCAAGCGAGC CCACACAGTG CACCATGCTG
    TATTCACGCC AAGGAACTAC GGAAACCATT GAGGAGGTAG AGGCTGAAGC TGAGGAGGAG
    GTGGTAGAGA GTCTGGAGCC TGAGCTCCAT GATGCCGAAG AGAAGGAATA TGCAGCTGAC
    TATGAAGCAG GGGTAGAGGA GGTCAGCCTC ACTCCAGATG AGGAGTTGCC TCAGTTCTCA
    ACAGATGACG GTGACGTGGA GGCCCCGCCC TCCTACAGCA AGGCTGTCAG CTTTGAGCAC
    CTCTCGTTTG CATCACAAGA TGACTCCGGG GGCAAGAACC ACATGGTGGT CAGCCCTGAC
    GACAGCCGCA CAGACAAGCT GGAGTCCAGT ATCTTACCGC CCCTGACTCA CGAGCTGACA
    GCCAGCGATC TGCTCATGAG CAAGATGTTC CGTGATGATG AGCTTGAAGA ATCTGAGAAA
    TTCTACGTGG ACCAGCCCCG GTTCTTGCTG CTATTCTATG CCATGTATAA CACTCTGGTA
    GCCCGCTCGG AAATGGTGTG CTATTTCGTG ATCATCCTGA ACCACATGAT CTCTGCCTCC
    ATCATTACTC TTCTGTTGCC CATCCTGATC TTCCTCTGGG CCATGCTGTC CGTCCCCAGG
    CCCAGCCGGC GCTTCTGGAT GATGGCCATC GTCTACACTG AGGTGGCGAT TGTAGTGAAA
    TATTTCTTCC AGTTTGGCTT CTTTCCTTGG AATAAGGACT TGGAAATTTA CAAGGACAGG
    CCCTACTTTC CACCAAACAT CATAGGGGTG GAAAAGAAAG AGGGCTATGT TCTTTACGAC
    CTCATTCAGC TGCTGGCTCT GTTCTTCCAT CGATCCATTC TGAAGTGTCA TGGCCTATGG
    GACGAGGATG ACATTGTTGA CAGTAACACA GACAAGGAAG GATCTGATGA TGAGCTGTCC
    CTCGGTCAGG GCAGAAGAGG CTCCTCAGAG TCACTGAAGT CCATCAACTT GGCTGCCTCT
    GTAGAGTCCG TCCATGTGAC CTTCCCAGAG CAGCCAGCAG CCATCCGGAG AAAACGCTCC
    TGCAGCAGCT CGCAGATCTC CCCACGGTCC AGCTTCTCTT CAAATAGGTC CAAAAGAGGC
    AGCACAAGCA CCCGAAACAG CAGCCAAAAA GGAAGCAGTG TTTTGAGCAT TAAACAAAAA
    AGCAAAAGAG AACTTTACAT GGAAAAGCTT CAAGAACATT TAATCAAAGC AAAGGCCTTT
    ACCATAAAGA AAACTCTACA AATCTATGTA CCCATTCGGC AATTCTTCTA TGACCTCATC
    CACCCAGACT ACAGTGCTGT GACTGACGTG TATGTGCTGA TGTTCCTGGC CGACACCGTG
    GACTTCATCA TCATCGTCTT TGGCTTCTGG GCTTTTGGGA AACACTCGGC AGCTGCAGAT
    ATCACCTCTT CCCTATCAGA AGACCAGGTC CCCGGGCCAT TCCTGGTGAT GGTTCTCATT
    CAGTTTGGAA CCATGGTGGT GGACCGTGCA CTCTACCTCA GGAAGACTGT GCTTGGCAAG
    GTCATTTTCC AGGTCATCCT CGTGTTTGGA ATTCACTTCT GGATGTTCTT CATCCTACCC
    AGTGTGACTG AGAGGAAATT CAGCCAGAAC CTGGTGGCAC AGCTCTGGTA CTTTGTGAAA
    TGTGTGTACT TTGGGTTGTC TGCCTACCAA ATCCGCTGTG GCTACCCAAC ACGTGTGCTC
    GGAAACTTCC TCACGAAGAG CTACAATTAT GTCAACCTCT TCTTGTTTCA AGGGTTTCGT
    CTCGTCCCAT TTCTGACAGA GCTGCGGGCT GTGATGGACT GGGTGTGGAC AGATACAACC
    TTGAGCCTCT CCAGCTGGAT TTGTGTGGAG GACATTTACG CACACATTTT CATCTTAAAG
    TGCTGGCGAG AGTCGGAAAA GAGATACCCT CAACCCAGGG GCCAGAAGAA GAAGAAAGCT
    GTGAAGTATG GCATGGGCGG AATGATCATT GTCCTGCTCA TCTGTATCGT CTGGTTTCCT
    CTGCTCTTCA TGTCTTTGAT CAAGTCTGTC GCAGGGGTTA TTAACCAACC CCTGGATGTG
    TCCGTCACAA TAACCCTGGG AGGCTATCAG CCTATCTTCA CAATGAGTGC CCAGCAAAGC
    CAGCTGAAAG TTATGGATGG TACAAAGTAT AACGAATTCA TAAAGTCTTT CGGCCCCAAC
    AGTGGCGCTA TGCAATTTCT GGAGAATTAT GAAAGGCAAG ACATAACAGT AGCGGAACTG
    GAAGGGAATT CGAATTCTTT GTGGACCATC AGCCCTCCCA GTAAGCAGAA AATGATACAG
    GAGCTCACCG ACCCCAATAG TTGCTTCTCT GTTGTTTTTT CATGGAGTAT CCAGAGAAAC
    ATGACTCTGG GTGCAAAGGC TGAAATAGCA ACGGACAAGC TTTCTTTTCC TCTCACGGAT
    GCGACACGAA GTAGCATAGC CAAAATGATT GCTGGCAATG ACACAGAAAG TTCCAACACA
    CCAGTGACAA TAGAAAAGAT CTACCCATAC TATGTGAAGG CACCCAGTGA CTCAAACTCA
    AAGCCTATAA AACAGCTTCT GTCAGAAAAC AATTTCATGA ATATCACCAT CATTTTGTTC
    AGAGACAATG TCACAAAGTC CAATAGTGAG TGGTGGGTTC TCAACCTGAC TGGAAGTAGG
    ATATTCAATC AGGGATCCCA AGCCTTGGAG CTGGTGGTCT TCAATGACAA AGTCAGCCCC
    CCAAGTCTGG GCTTCTTGGC TGGCTATGGC ATCATGGGAT TGTATGCATC CGTTGTCCTT
    GTAATTGGGA AGTTTGTTCG CGAATTCTTC AGTGGGATTT CTCACTCTAT CATGTTTGAA
    GAGCTTCCAA ATGTGGACAG AATTTTGAAG TTATGCACAG ATATATTTCT CGTACGGGAG
    ACAGGGGAAC TGGAGCTGGA GGAAGACCTC TATGCCAAAT TGATATTCCT GTACAGGTCG
    CCAGAAACCA TGATCAAGTG GACTAGAGAA AAAACAAATT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017456608.1
    CDS940..9441
    Translation

    Target ORF information:

    RefSeq Version XM_017601119.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017601119.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTCCCCAT CTGCCTGGCA 
    GTAGCATGTG CATTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCG ACGATGCAAG GACACACAGG GCGGCTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCCTTT CTGCTCCTTC ACATCATTTT CCACATCACA
    CTGGCCAGCC TGGAAGCTCA GCACCGCATC GCACCCGCTT ACAACTGCTC AACTTGGGAA
    AAGACCTTCC GGCAGATCGG CTTCGAAAGC TTGAAGGGAG CTGATGCTGG CAATGGCATC
    AGGGTGTTTG TGCCTGATAT CGGGATGTTC ATTGCGAGCC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACGGAA GAAGTAGCCC AATTTAATTC CGAGTGCGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GACTCAGAAG AAGCGCTGAT TTATGAGGAG
    GACTTAGATG GGGAAGAAGG CATGGAAGGC GAGCTGGAAG AAAGCACAAA GTTAAAAATG
    TTTCGCAGGC TCGCCTCCGT CGCTTCCAAG CTTAAGGAGT TCATCGGCAA TATGATAACC
    ACGGCGGGCA AGGTGGTGGT GACCATCCTG TTGGGCTCCT CTGGCATGAT GCTGCCATCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT CTGGGTCTGT GCACCTGGTG GTCCTGGTGC
    CGAACTTTTG ACCCATTGCT GTTTGGCTGC CTCTGTGTCC TGTTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTTCAGTTCT TCCAAGAAGC AGTCCCACCC
    AATGACTACT ATGCAAGGTT GTTTGGAATC AAATCCGTTA TTCAAACAGA CTGCTCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTACAC GCTGGCCACG CTGATCCGCA TTTGGTTGCA AGAGCCTCTT
    GTGCAGGAGG AGATGTCGAA GGAAGAGGAG GGTGCACTGG ATGGCTGCTC CAATCAGAAC
    ACAGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    CTTTTATCCA TGACCCAAGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACTGTGAAC
    GGCAACCCTG TGGACTACCA CACCATCCAC CCAAGTCTGC CCATAGAGAA TGGCCCCGCC
    AAGACGGACT TGTATACTAC TCCTCAGTAC CGGTGGGAGC CCTCAGAGGA GTCCTCAGAA
    AAGAAAGAGG AGGAAGAGGA CAAGAGAGAA GACTCAGAAG GGGAAGGGAG CCAAGAGGAA
    AAACGGAGTG TGAGAATTCA TGCCATGGTC GCCGTGTTCC AGTTCATCAT GAAGCAGAGT
    TACATCTGTG CCCTCATCGC CATGATGGCA TGGAGCATCA CCTACCACAG CTGGCTGACA
    TTTGTGCTGT TGATCTGGTC GTGCACTCTC TGGATGATTC GCAACAGGAG GAAGTACGCC
    ATGATCAGCT CTCCGTTCAT GGTTGTCTAT GCCAATCTGC TGCTGGTACT GCAGTACATA
    TGGAGCTTTG AACTTCCAGA AATCAAGAAA GTCCCTGGAT TTCTAGAAAA GAAAGAGCCT
    GGAGAGCTTG CCTCAAAGAT CCTCTTCACC ATCACATTTT GGCTGCTCCT GAGGCAGCAT
    CTCACAGAGC AGAAGGCCCT TCAGGAAAAG GAAGCACTTC TGTCTGAAGT CAAAATTGGA
    AGTCAAGAAC TTGACGAAAA GGATGAGGAA CTCCAAGATA TACAAGTGGA AGGAGAGCCC
    ATAGAGAAGG AAGAAGAGGA GGAGGAAATA AAGGAAGACA GACATGAGGT AAAGAAGGAA
    GAGGAAGAGG AAGAGGAAGA TGACGATGAC CAGGACATCA TGAAGGTTCT GGGCAATCTG
    GTGGTGGCCC TCTTCATCAA GTATTGGATC TACGTCTGCG GGGGCATGTT CTTTTTTGTC
    AGCTTTGAGG GGAAAATCGT CATGTACAAA ATCATCTACA TGGTGCTGTT CCTGTTCTGT
    GTGGCCTTGT ACCAGGTGCA CTACGAGTGG TGGAGGAAGA TCCTGAAATA CTTCTGGATG
    TCGGTGGTCA TTTACACCAT GCTGGTGCTC ATCTTCATCT ACACTTACCA GTTTGAGAAC
    TTCCCAGGCC TGTGGCAAAA CATGACCGGG TTAAAGAAAG AGAAGCTTGA GGATCTGGGC
    CTGAAGCAGT TCACTGTGGC CGAACTGTTC ACACGAATAT TCATCCCCAC CTCCTTTCTG
    CTGGTCTGCA TCCTGCACCT CCACTATTTC CATGACCGGT TCCTGGAACT CACAGACCTC
    AAGTCCATCC CCAGCAAAGA AGACAACACC ATCTACAGCC ATGCCAAGGT CAATGGTCGC
    GTGTACCTAA TAATAAATAG CATCAAGAAA AAATTACCAA TCCACCAGAA TGAGTTAGCC
    CACCCTGAAG GAAGCCTCCC AGACCTAGCC ATTATGAACA TGACGGCCAG CCTCGATAAG
    CCTGAGAGGC AGAAGTTGGC AGAGCCTGGG GAGGAGAGGA CAGAAGAATG TGCCAAGAAG
    GCAGAGAAGG GTGAGGCTGG GAAAGACAGT GATGAGTCTG AGGAGGAGGA AGGTGAGGAA
    GAGGAATCCG AGGAGGAAGA ATCCTCAGAT CTCAGGAACA AGTGGCACCT CGTGATTGAC
    CGGCTCACTG TGCTCTTTCT AAAGTTCCTG GAGTATTTCC ACAAGTTGCA GGTGTTTATG
    TGGTGGATCT TGGAGCTGCA CATCATCAAA ATCGTCTCAT CGTACATTAT CTGGGTGACT
    GTCAAAGAGG TGTCTCTGTT CAACTATGTA TTTTTGATTT CTTGGGCATT TGCTCTGCCA
    TATGCCAAAC TCCGCCGTGT GGCTTCCAGT GTCTGCACTG TCTGGACGTG TGTGATCATC
    GTCTGCAAAA TGTTGTACCA GCTGCAAACC ATTAAGCCTG AGAACTTTTC TGTCAACTGC
    TCCTTGCCCA ATGAAAATCA AACCAACATA CCCCTTAATG AGTTGAACAA GTCTCTTCTC
    TACAGTGCCC CTGTTGATCC CACTGAGTGG GTTGGCCTGA GGAAGTCCTC CCCTTTACTC
    GTCTACCTGA GGAACAACCT CCTGATGTTG GCGATTTTGG CCTTCGAAGT CACTGTTTAC
    CGACATCAGG AGTACTATCG AGGCCGAAAC AACTTGACTG CTCCGGTGTC TAAAACTATC
    TTTCATGACA TCACAAGGTT ACATCTAGAT GATGGGCTTA TCAATTGTGC CAAATATTTT
    GTAAACTACT TCTTCTACAA GTTTGGCCTA GAGACCTGTT TCCTAATGTC AGTGAACGTA
    ATTGGACAGC GAATGGACTT CTACGCCATG ATTCATGCCT GTTGGCTGAT CGGTGTCTTG
    TACCGTCGCA GAAGAAAAGC CATTGCTGAG GTCTGGCCCA AGTACTGCTG CTTCCTGGCT
    TGCATCATCA CCTTCCAGTA CTTTGTCTGC ATAGGCATCC CTCCTGCCCC CTGCCGAGAT
    TACCCATGGA GGTTCAAGGG TGCCAACTTC AATGACAACA TCATCAAGTG GCTGTACTTC
    CCAGATTTCA TCGTGCGGCC CAACCCTGTG TTTCTTGTCT ATGACTTCAT GCTGCTCCTG
    TGTGCTTCCT TACAACGACA GATTTTTGAG GACGAAAACA AGGCAGCTGT GCGCATCATG
    GCCGGGGACA ATGTCGAGAT TTGCATGAAC CTTGACGCGG CCTCATTCAG CCAGCATAAC
    CCTGTACCAG ATTTCATTCA CTGCAGATCT TACTTGGACA TGTCCAAAGT GATCATCTTC
    AGCTACCTCT TCTGGTTCGT CCTCACCATC ATCTTCATTA CCGGAACGAC CAGGATCAGC
    ATCTTTTGCA TGGGTTACCT GGTGGCCTGT TTCTATTTCC TGCTCTTTGG AGGTGATCTG
    CTCCTGAAAC CCATCAAGAG CATCCTACGC TACTGGGACT GGCTGATTGC ATACAACGTG
    TTTGTGATCA CCATGAAAAA CATACTGTCA ATAGGAGCCT GTGGATACAT CGGTGCACTG
    GTGAGAAACA GCTGCTGGCT GATCCAAGCG TTCAGCCTGG CCTGCACGGT GAAAGGCTAT
    AAAATGCCTG AAGATGACTC CAGATGCAAG CTGCCCAGTG GGGAGGCAGG GATCATCTGG
    GACAGCATCT GCTTTGCGTT TCTCCTGCTA CAGAGGAGAG TCTTCATGAG TTACTATTTC
    CTGCATGTCG TGGCTGACAT TAAAGCATCC CAGATCCTGG CATCCAGAGG GGCCGAACTT
    TTCCAGGCTA CAATTGTGAA AGCTGTAAAG GCAAGAATTG AAGAAGAGAA AAAATCCATG
    GACCAGCTGA AGAGACAGAT GGATCGCATC AAAGCCCGGC AGCAAAAGTA CAAAAAGGGT
    AAGGAGAGGA TGCTGAGCTT GACCCAGGAG TCAGGGGAAG GCCAGGACAT CCAGAAAGTC
    TCAGAAGAAG ACGATGAAAG AGAAGCAGAC AAACAGAAAG CCAAGGGCAA AAAAAAGCAG
    TGGTGGCGGC CTTGGGTTGA TCATGCTTCC ATGGTCAGGA GTGGAGATTA TTATTTGTTT
    GAAACGGATA GTGAAGAGGA GGAAGAAGAG GAACTCAAAA AGGAGGATGA AGAACCACCA
    CGGAAGTCTG CATTCCAGTT CGTCTACCAA GCCTGGATTA CCGATCCTAA AACAGCACTC
    CGACAGAGGA GGAAAGAGAA GAAGAAAATG GCCAGAGAAG AGCAGAAGGG AAGACGCAAA
    GGATCTGGGG AGGGTCCAGT GGAATGGGAA GACAGAGAAG ATGAGCCAAT CAAAAAGAAA
    TCTGATGGAC CAGATAATAT CATCAAGAGG ATATTTAATA TCCTGAAGTT CACCTGGGTC
    CTGTTCCTGG CCACAGTGGA CAGTTTCACT ACTTGGCTCA ACTCCATCTC AAGAGAGCAT
    ATCGACATAT CCACAGTTCT GCGGATTGAA AGATGCATGC TGACCCGAGA GATAAAGAAG
    GGCAACGTTC CGACACGGGA AAGCATCCAC ATGTACTACC AGAACCACAT GATGAACCTT
    TCCAGAGAAT CCGGACTGGA CACAATCGAC GAGCACTCTG GCGCTGGTTC CAGGGCCCAG
    GCAGCACACA GGATGGATAG CTTAGATTCC CATGACAGTA TCTCCAGCTG TTACACTGAA
    GCAACCCTGC TGATCTCAAG GCAGTCCACC CTTGATGATT TAGATGGACA AGACCCTGTT
    CCTAAAACAA GCGAGCGTGC TCGGCCTAGG CTCCGTAAAG TGTTCAGCAT GGACATGTCC
    TCCTCATCAG CTGACAGTGG CAGTGTAGCA TCAAGCGAGC CCACACAGTG CACCATGCTG
    TATTCACGCC AAGGAACTAC GGAAACCATT GAGGAGGTAG AGGCTGAAGC TGAGGAGGAG
    GTGGTAGAGA GTCTGGAGCC TGAGCTCCAT GATGCCGAAG AGAAGGAATA TGCAGCTGAC
    TATGAAGCAG GGGTAGAGGA GGTCAGCCTC ACTCCAGATG AGGAGTTGCC TCAGTTCTCA
    ACAGATGACG GTGACGTGGA GGCCCCGCCC TCCTACAGCA AGGCTGTCAG CTTTGAGCAC
    CTCTCGTTTG CATCACAAGA TGACTCCGGG GGCAAGAACC ACATGGTGGT CAGCCCTGAC
    GACAGCCGCA CAGACAAGCT GGAGTCCAGT ATCTTACCGC CCCTGACTCA CGAGCTGACA
    GCCAGCGATC TGCTCATGAG CAAGATGTTC CGTGATGATG AGCTTGAAGA ATCTGAGAAA
    TTCTACGTGG ACCAGCCCCG GTTCTTGCTG CTATTCTATG CCATGTATAA CACTCTGGTA
    GCCCGCTCGG AAATGGTGTG CTATTTCGTG ATCATCCTGA ACCACATGAT CTCTGCCTCC
    ATCATTACTC TTCTGTTGCC CATCCTGATC TTCCTCTGGG CCATGCTGTC CGTCCCCAGG
    CCCAGCCGGC GCTTCTGGAT GATGGCCATC GTCTACACTG AGGTGGCGAT TGTAGTGAAA
    TATTTCTTCC AGTTTGGCTT CTTTCCTTGG AATAAGGACT TGGAAATTTA CAAGGACAGG
    CCCTACTTTC CACCAAACAT CATAGGGGTG GAAAAGAAAG AGGGCTATGT TCTTTACGAC
    CTCATTCAGC TGCTGGCTCT GTTCTTCCAT CGATCCATTC TGAAGTGTCA TGGCCTATGG
    GACGAGGATG ACATTGTTGA CAGTAACACA GACAAGGAAG GATCTGATGA TGAGCTGTCC
    CTCGGTCAGG GCAGAAGAGG CTCCTCAGAG TCACTGAAGT CCATCAACTT GGCTGCCTCT
    GTAGAGTCCG TCCATGTGAC CTTCCCAGAG CAGCCAGCAG CCATCCGGAG AAAACGCTCC
    TGCAGCAGCT CGCAGATCTC CCCACGGTCC AGCTTCTCTT CAAATAGGTC CAAAAGAGGC
    AGCACAAGCA CCCGAAACAG CAGCCAAAAA GGAAGCAGTG TTTTGAGCAT TAAACAAAAA
    AGCAAAAGAG AACTTTACAT GGAAAAGCTT CAAGAACATT TAATCAAAGC AAAGGCCTTT
    ACCATAAAGA AAACTCTACA AATCTATGTA CCCATTCGGC AATTCTTCTA TGACCTCATC
    CACCCAGACT ACAGTGCTGT GACTGACGTG TATGTGCTGA TGTTCCTGGC CGACACCGTG
    GACTTCATCA TCATCGTCTT TGGCTTCTGG GCTTTTGGGA AACACTCGGC AGCTGCAGAT
    ATCACCTCTT CCCTATCAGA AGACCAGGTC CCCGGGCCAT TCCTGGTGAT GGTTCTCATT
    CAGTTTGGAA CCATGGTGGT GGACCGTGCA CTCTACCTCA GGAAGACTGT GCTTGGCAAG
    GTCATTTTCC AGGTCATCCT CGTGTTTGGA ATTCACTTCT GGATGTTCTT CATCCTACCC
    AGTGTGACTG AGAGGAAATT CAGCCAGAAC CTGGTGGCAC AGCTCTGGTA CTTTGTGAAA
    TGTGTGTACT TTGGGTTGTC TGCCTACCAA ATCCGCTGTG GCTACCCAAC ACGTGTGCTC
    GGAAACTTCC TCACGAAGAG CTACAATTAT GTCAACCTCT TCTTGTTTCA AGGGTTTCGT
    CTCGTCCCAT TTCTGACAGA GCTGCGGGCT GTGATGGACT GGGTGTGGAC AGATACAACC
    TTGAGCCTCT CCAGCTGGAT TTGTGTGGAG GACATTTACG CACACATTTT CATCTTAAAG
    TGCTGGCGAG AGTCGGAAAA GAGATACCCT CAACCCAGGG GCCAGAAGAA GAAGAAAGCT
    GTGAAGTATG GCATGGGCGG AATGATCATT GTCCTGCTCA TCTGTATCGT CTGGTTTCCT
    CTGCTCTTCA TGTCTTTGAT CAAGTCTGTC GCAGGGGTTA TTAACCAACC CCTGGATGTG
    TCCGTCACAA TAACCCTGGG AGGCTATCAG CCTATCTTCA CAATGAGTGC CCAGCAAAGC
    CAGCTGAAAG TTATGGATGG TACAAAGTAT AACGAATTCA TAAAGTCTTT CGGCCCCAAC
    AGTGGCGCTA TGCAATTTCT GGAGAATTAT GAAAGGCAAG ACATAACAGT AGCGGAACTG
    GAAGGGAATT CGAATTCTTT GTGGACCATC AGCCCTCCCA GTAAGCAGAA AATGATACAG
    GAGCTCACCG ACCCCAATAG TTGCTTCTCT GTTGTTTTTT CATGGAGTAT CCAGAGAAAC
    ATGACTCTGG GTGCAAAGGC TGAAATAGCA ACGGACAAGC TTTCTTTTCC TCTCACGGAT
    GCGACACGAA GTAGCATAGC CAAAATGATT GCTGGCAATG ACACAGAAAG TTCCAACACA
    CCAGTGACAA TAGAAAAGAT CTACCCATAC TATGTGAAGG CACCCAGTGA CTCAAACTCA
    AAGCCTATAA AACAGCTTCT GTCAGAAAAC AATTTCATGA ATATCACCAT CATTTTGTTC
    AGAGACAATG TCACAAAGTC CAATAGTGAG TGGTGGGTTC TCAACCTGAC TGGAAGTAGG
    ATATTCAATC AGGGATCCCA AGCCTTGGAG CTGGTGGTCT TCAATGACAA AGTCAGCCCC
    CCAAGTCTGG GCTTCTTGGC TGGCTATGGC ATCATGGGAT TGTATGCATC CGTTGTCCTT
    GTAATTGGGA AGTTTGTTCG CGAATTCTTC AGTGGGATTT CTCACTCTAT CATGTTTGAA
    GAGCTTCCAA ATGTGGACAG AATTTTGAAG TTATGCACAG ATATATTTCT CGTACGGGAG
    ACAGGGGAAC TGGAGCTGGA GGAAGACCTC TATGCCAAAT TGATATTCCT GTACAGGTCG
    CCAGAAACCA TGATCAAGTG GACTAGAGAA AAAACAAATT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa54721
    Clone ID Related Accession (Same CDS sequence) XM_017601119.1 , XM_017587880.1
    Accession Version XM_017587880.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8502bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product piezo-type mechanosensitive ion channel component 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000086.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTCCCCAT CTGCCTGGCA 
    GTAGCATGTG CATTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCG ACGATGCAAG GACACACAGG GCGGCTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCCTTT CTGCTCCTTC ACATCATTTT CCACATCACA
    CTGGCCAGCC TGGAAGCTCA GCACCGCATC GCACCCGCTT ACAACTGCTC AACTTGGGAA
    AAGACCTTCC GGCAGATCGG CTTCGAAAGC TTGAAGGGAG CTGATGCTGG CAATGGCATC
    AGGGTGTTTG TGCCTGATAT CGGGATGTTC ATTGCGAGCC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACGGAA GAAGTAGCCC AATTTAATTC CGAGTGCGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GACTCAGAAG AAGCGCTGAT TTATGAGGAG
    GACTTAGATG GGGAAGAAGG CATGGAAGGC GAGCTGGAAG AAAGCACAAA GTTAAAAATG
    TTTCGCAGGC TCGCCTCCGT CGCTTCCAAG CTTAAGGAGT TCATCGGCAA TATGATAACC
    ACGGCGGGCA AGGTGGTGGT GACCATCCTG TTGGGCTCCT CTGGCATGAT GCTGCCATCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT CTGGGTCTGT GCACCTGGTG GTCCTGGTGC
    CGAACTTTTG ACCCATTGCT GTTTGGCTGC CTCTGTGTCC TGTTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTTCAGTTCT TCCAAGAAGC AGTCCCACCC
    AATGACTACT ATGCAAGGTT GTTTGGAATC AAATCCGTTA TTCAAACAGA CTGCTCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTACAC GCTGGCCACG CTGATCCGCA TTTGGTTGCA AGAGCCTCTT
    GTGCAGGAGG AGATGTCGAA GGAAGAGGAG GGTGCACTGG ATGGCTGCTC CAATCAGAAC
    ACAGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    CTTTTATCCA TGACCCAAGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACTGTGAAC
    GGCAACCCTG TGGACTACCA CACCATCCAC CCAAGTCTGC CCATAGAGAA TGGCCCCGCC
    AAGACGGACT TGTATACTAC TCCTCAGTAC CGGTGGGAGC CCTCAGAGGA GTCCTCAGAA
    AAGAAAGAGG AGGAAGAGGA CAAGAGAGAA GACTCAGAAG GGGAAGGGAG CCAAGAGGAA
    AAACGGAGTG TGAGAATTCA TGCCATGGTC GCCGTGTTCC AGTTCATCAT GAAGCAGAGT
    TACATCTGTG CCCTCATCGC CATGATGGCA TGGAGCATCA CCTACCACAG CTGGCTGACA
    TTTGTGCTGT TGATCTGGTC GTGCACTCTC TGGATGATTC GCAACAGGAG GAAGTACGCC
    ATGATCAGCT CTCCGTTCAT GGTTGTCTAT GCCAATCTGC TGCTGGTACT GCAGTACATA
    TGGAGCTTTG AACTTCCAGA AATCAAGAAA GTCCCTGGAT TTCTAGAAAA GAAAGAGCCT
    GGAGAGCTTG CCTCAAAGAT CCTCTTCACC ATCACATTTT GGCTGCTCCT GAGGCAGCAT
    CTCACAGAGC AGAAGGCCCT TCAGGAAAAG GAAGCACTTC TGTCTGAAGT CAAAATTGGA
    AGTCAAGAAC TTGACGAAAA GGATGAGGAA CTCCAAGATA TACAAGTGGA AGGAGAGCCC
    ATAGAGAAGG AAGAAGAGGA GGAGGAAATA AAGGAAGACA GACATGAGGT AAAGAAGGAA
    GAGGAAGAGG AAGAGGAAGA TGACGATGAC CAGGACATCA TGAAGGTTCT GGGCAATCTG
    GTGGTGGCCC TCTTCATCAA GTATTGGATC TACGTCTGCG GGGGCATGTT CTTTTTTGTC
    AGCTTTGAGG GGAAAATCGT CATGTACAAA ATCATCTACA TGGTGCTGTT CCTGTTCTGT
    GTGGCCTTGT ACCAGGTGCA CTACGAGTGG TGGAGGAAGA TCCTGAAATA CTTCTGGATG
    TCGGTGGTCA TTTACACCAT GCTGGTGCTC ATCTTCATCT ACACTTACCA GTTTGAGAAC
    TTCCCAGGCC TGTGGCAAAA CATGACCGGG TTAAAGAAAG AGAAGCTTGA GGATCTGGGC
    CTGAAGCAGT TCACTGTGGC CGAACTGTTC ACACGAATAT TCATCCCCAC CTCCTTTCTG
    CTGGTCTGCA TCCTGCACCT CCACTATTTC CATGACCGGT TCCTGGAACT CACAGACCTC
    AAGTCCATCC CCAGCAAAGA AGACAACACC ATCTACAGCC ATGCCAAGGT CAATGGTCGC
    GTGTACCTAA TAATAAATAG CATCAAGAAA AAATTACCAA TCCACCAGAA TGAGTTAGCC
    CACCCTGAAG GAAGCCTCCC AGACCTAGCC ATTATGAACA TGACGGCCAG CCTCGATAAG
    CCTGAGAGGC AGAAGTTGGC AGAGCCTGGG GAGGAGAGGA CAGAAGAATG TGCCAAGAAG
    GCAGAGAAGG GTGAGGCTGG GAAAGACAGT GATGAGTCTG AGGAGGAGGA AGGTGAGGAA
    GAGGAATCCG AGGAGGAAGA ATCCTCAGAT CTCAGGAACA AGTGGCACCT CGTGATTGAC
    CGGCTCACTG TGCTCTTTCT AAAGTTCCTG GAGTATTTCC ACAAGTTGCA GGTGTTTATG
    TGGTGGATCT TGGAGCTGCA CATCATCAAA ATCGTCTCAT CGTACATTAT CTGGGTGACT
    GTCAAAGAGG TGTCTCTGTT CAACTATGTA TTTTTGATTT CTTGGGCATT TGCTCTGCCA
    TATGCCAAAC TCCGCCGTGT GGCTTCCAGT GTCTGCACTG TCTGGACGTG TGTGATCATC
    GTCTGCAAAA TGTTGTACCA GCTGCAAACC ATTAAGCCTG AGAACTTTTC TGTCAACTGC
    TCCTTGCCCA ATGAAAATCA AACCAACATA CCCCTTAATG AGTTGAACAA GTCTCTTCTC
    TACAGTGCCC CTGTTGATCC CACTGAGTGG GTTGGCCTGA GGAAGTCCTC CCCTTTACTC
    GTCTACCTGA GGAACAACCT CCTGATGTTG GCGATTTTGG CCTTCGAAGT CACTGTTTAC
    CGACATCAGG AGTACTATCG AGGCCGAAAC AACTTGACTG CTCCGGTGTC TAAAACTATC
    TTTCATGACA TCACAAGGTT ACATCTAGAT GATGGGCTTA TCAATTGTGC CAAATATTTT
    GTAAACTACT TCTTCTACAA GTTTGGCCTA GAGACCTGTT TCCTAATGTC AGTGAACGTA
    ATTGGACAGC GAATGGACTT CTACGCCATG ATTCATGCCT GTTGGCTGAT CGGTGTCTTG
    TACCGTCGCA GAAGAAAAGC CATTGCTGAG GTCTGGCCCA AGTACTGCTG CTTCCTGGCT
    TGCATCATCA CCTTCCAGTA CTTTGTCTGC ATAGGCATCC CTCCTGCCCC CTGCCGAGAT
    TACCCATGGA GGTTCAAGGG TGCCAACTTC AATGACAACA TCATCAAGTG GCTGTACTTC
    CCAGATTTCA TCGTGCGGCC CAACCCTGTG TTTCTTGTCT ATGACTTCAT GCTGCTCCTG
    TGTGCTTCCT TACAACGACA GATTTTTGAG GACGAAAACA AGGCAGCTGT GCGCATCATG
    GCCGGGGACA ATGTCGAGAT TTGCATGAAC CTTGACGCGG CCTCATTCAG CCAGCATAAC
    CCTGTACCAG ATTTCATTCA CTGCAGATCT TACTTGGACA TGTCCAAAGT GATCATCTTC
    AGCTACCTCT TCTGGTTCGT CCTCACCATC ATCTTCATTA CCGGAACGAC CAGGATCAGC
    ATCTTTTGCA TGGGTTACCT GGTGGCCTGT TTCTATTTCC TGCTCTTTGG AGGTGATCTG
    CTCCTGAAAC CCATCAAGAG CATCCTACGC TACTGGGACT GGCTGATTGC ATACAACGTG
    TTTGTGATCA CCATGAAAAA CATACTGTCA ATAGGAGCCT GTGGATACAT CGGTGCACTG
    GTGAGAAACA GCTGCTGGCT GATCCAAGCG TTCAGCCTGG CCTGCACGGT GAAAGGCTAT
    AAAATGCCTG AAGATGACTC CAGATGCAAG CTGCCCAGTG GGGAGGCAGG GATCATCTGG
    GACAGCATCT GCTTTGCGTT TCTCCTGCTA CAGAGGAGAG TCTTCATGAG TTACTATTTC
    CTGCATGTCG TGGCTGACAT TAAAGCATCC CAGATCCTGG CATCCAGAGG GGCCGAACTT
    TTCCAGGCTA CAATTGTGAA AGCTGTAAAG GCAAGAATTG AAGAAGAGAA AAAATCCATG
    GACCAGCTGA AGAGACAGAT GGATCGCATC AAAGCCCGGC AGCAAAAGTA CAAAAAGGGT
    AAGGAGAGGA TGCTGAGCTT GACCCAGGAG TCAGGGGAAG GCCAGGACAT CCAGAAAGTC
    TCAGAAGAAG ACGATGAAAG AGAAGCAGAC AAACAGAAAG CCAAGGGCAA AAAAAAGCAG
    TGGTGGCGGC CTTGGGTTGA TCATGCTTCC ATGGTCAGGA GTGGAGATTA TTATTTGTTT
    GAAACGGATA GTGAAGAGGA GGAAGAAGAG GAACTCAAAA AGGAGGATGA AGAACCACCA
    CGGAAGTCTG CATTCCAGTT CGTCTACCAA GCCTGGATTA CCGATCCTAA AACAGCACTC
    CGACAGAGGA GGAAAGAGAA GAAGAAAATG GCCAGAGAAG AGCAGAAGGG AAGACGCAAA
    GGATCTGGGG AGGGTCCAGT GGAATGGGAA GACAGAGAAG ATGAGCCAAT CAAAAAGAAA
    TCTGATGGAC CAGATAATAT CATCAAGAGG ATATTTAATA TCCTGAAGTT CACCTGGGTC
    CTGTTCCTGG CCACAGTGGA CAGTTTCACT ACTTGGCTCA ACTCCATCTC AAGAGAGCAT
    ATCGACATAT CCACAGTTCT GCGGATTGAA AGATGCATGC TGACCCGAGA GATAAAGAAG
    GGCAACGTTC CGACACGGGA AAGCATCCAC ATGTACTACC AGAACCACAT GATGAACCTT
    TCCAGAGAAT CCGGACTGGA CACAATCGAC GAGCACTCTG GCGCTGGTTC CAGGGCCCAG
    GCAGCACACA GGATGGATAG CTTAGATTCC CATGACAGTA TCTCCAGCTG TTACACTGAA
    GCAACCCTGC TGATCTCAAG GCAGTCCACC CTTGATGATT TAGATGGACA AGACCCTGTT
    CCTAAAACAA GCGAGCGTGC TCGGCCTAGG CTCCGTAAAG TGTTCAGCAT GGACATGTCC
    TCCTCATCAG CTGACAGTGG CAGTGTAGCA TCAAGCGAGC CCACACAGTG CACCATGCTG
    TATTCACGCC AAGGAACTAC GGAAACCATT GAGGAGGTAG AGGCTGAAGC TGAGGAGGAG
    GTGGTAGAGA GTCTGGAGCC TGAGCTCCAT GATGCCGAAG AGAAGGAATA TGCAGCTGAC
    TATGAAGCAG GGGTAGAGGA GGTCAGCCTC ACTCCAGATG AGGAGTTGCC TCAGTTCTCA
    ACAGATGACG GTGACGTGGA GGCCCCGCCC TCCTACAGCA AGGCTGTCAG CTTTGAGCAC
    CTCTCGTTTG CATCACAAGA TGACTCCGGG GGCAAGAACC ACATGGTGGT CAGCCCTGAC
    GACAGCCGCA CAGACAAGCT GGAGTCCAGT ATCTTACCGC CCCTGACTCA CGAGCTGACA
    GCCAGCGATC TGCTCATGAG CAAGATGTTC CGTGATGATG AGCTTGAAGA ATCTGAGAAA
    TTCTACGTGG ACCAGCCCCG GTTCTTGCTG CTATTCTATG CCATGTATAA CACTCTGGTA
    GCCCGCTCGG AAATGGTGTG CTATTTCGTG ATCATCCTGA ACCACATGAT CTCTGCCTCC
    ATCATTACTC TTCTGTTGCC CATCCTGATC TTCCTCTGGG CCATGCTGTC CGTCCCCAGG
    CCCAGCCGGC GCTTCTGGAT GATGGCCATC GTCTACACTG AGGTGGCGAT TGTAGTGAAA
    TATTTCTTCC AGTTTGGCTT CTTTCCTTGG AATAAGGACT TGGAAATTTA CAAGGACAGG
    CCCTACTTTC CACCAAACAT CATAGGGGTG GAAAAGAAAG AGGGCTATGT TCTTTACGAC
    CTCATTCAGC TGCTGGCTCT GTTCTTCCAT CGATCCATTC TGAAGTGTCA TGGCCTATGG
    GACGAGGATG ACATTGTTGA CAGTAACACA GACAAGGAAG GATCTGATGA TGAGCTGTCC
    CTCGGTCAGG GCAGAAGAGG CTCCTCAGAG TCACTGAAGT CCATCAACTT GGCTGCCTCT
    GTAGAGTCCG TCCATGTGAC CTTCCCAGAG CAGCCAGCAG CCATCCGGAG AAAACGCTCC
    TGCAGCAGCT CGCAGATCTC CCCACGGTCC AGCTTCTCTT CAAATAGGTC CAAAAGAGGC
    AGCACAAGCA CCCGAAACAG CAGCCAAAAA GGAAGCAGTG TTTTGAGCAT TAAACAAAAA
    AGCAAAAGAG AACTTTACAT GGAAAAGCTT CAAGAACATT TAATCAAAGC AAAGGCCTTT
    ACCATAAAGA AAACTCTACA AATCTATGTA CCCATTCGGC AATTCTTCTA TGACCTCATC
    CACCCAGACT ACAGTGCTGT GACTGACGTG TATGTGCTGA TGTTCCTGGC CGACACCGTG
    GACTTCATCA TCATCGTCTT TGGCTTCTGG GCTTTTGGGA AACACTCGGC AGCTGCAGAT
    ATCACCTCTT CCCTATCAGA AGACCAGGTC CCCGGGCCAT TCCTGGTGAT GGTTCTCATT
    CAGTTTGGAA CCATGGTGGT GGACCGTGCA CTCTACCTCA GGAAGACTGT GCTTGGCAAG
    GTCATTTTCC AGGTCATCCT CGTGTTTGGA ATTCACTTCT GGATGTTCTT CATCCTACCC
    AGTGTGACTG AGAGGAAATT CAGCCAGAAC CTGGTGGCAC AGCTCTGGTA CTTTGTGAAA
    TGTGTGTACT TTGGGTTGTC TGCCTACCAA ATCCGCTGTG GCTACCCAAC ACGTGTGCTC
    GGAAACTTCC TCACGAAGAG CTACAATTAT GTCAACCTCT TCTTGTTTCA AGGGTTTCGT
    CTCGTCCCAT TTCTGACAGA GCTGCGGGCT GTGATGGACT GGGTGTGGAC AGATACAACC
    TTGAGCCTCT CCAGCTGGAT TTGTGTGGAG GACATTTACG CACACATTTT CATCTTAAAG
    TGCTGGCGAG AGTCGGAAAA GAGATACCCT CAACCCAGGG GCCAGAAGAA GAAGAAAGCT
    GTGAAGTATG GCATGGGCGG AATGATCATT GTCCTGCTCA TCTGTATCGT CTGGTTTCCT
    CTGCTCTTCA TGTCTTTGAT CAAGTCTGTC GCAGGGGTTA TTAACCAACC CCTGGATGTG
    TCCGTCACAA TAACCCTGGG AGGCTATCAG CCTATCTTCA CAATGAGTGC CCAGCAAAGC
    CAGCTGAAAG TTATGGATGG TACAAAGTAT AACGAATTCA TAAAGTCTTT CGGCCCCAAC
    AGTGGCGCTA TGCAATTTCT GGAGAATTAT GAAAGGCAAG ACATAACAGT AGCGGAACTG
    GAAGGGAATT CGAATTCTTT GTGGACCATC AGCCCTCCCA GTAAGCAGAA AATGATACAG
    GAGCTCACCG ACCCCAATAG TTGCTTCTCT GTTGTTTTTT CATGGAGTAT CCAGAGAAAC
    ATGACTCTGG GTGCAAAGGC TGAAATAGCA ACGGACAAGC TTTCTTTTCC TCTCACGGAT
    GCGACACGAA GTAGCATAGC CAAAATGATT GCTGGCAATG ACACAGAAAG TTCCAACACA
    CCAGTGACAA TAGAAAAGAT CTACCCATAC TATGTGAAGG CACCCAGTGA CTCAAACTCA
    AAGCCTATAA AACAGCTTCT GTCAGAAAAC AATTTCATGA ATATCACCAT CATTTTGTTC
    AGAGACAATG TCACAAAGTC CAATAGTGAG TGGTGGGTTC TCAACCTGAC TGGAAGTAGG
    ATATTCAATC AGGGATCCCA AGCCTTGGAG CTGGTGGTCT TCAATGACAA AGTCAGCCCC
    CCAAGTCTGG GCTTCTTGGC TGGCTATGGC ATCATGGGAT TGTATGCATC CGTTGTCCTT
    GTAATTGGGA AGTTTGTTCG CGAATTCTTC AGTGGGATTT CTCACTCTAT CATGTTTGAA
    GAGCTTCCAA ATGTGGACAG AATTTTGAAG TTATGCACAG ATATATTTCT CGTACGGGAG
    ACAGGGGAAC TGGAGCTGGA GGAAGACCTC TATGCCAAAT TGATATTCCT GTACAGGTCG
    CCAGAAACCA TGATCAAGTG GACTAGAGAA AAAACAAATT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017443369.1
    CDS940..9441
    Translation

    Target ORF information:

    RefSeq Version XM_017587880.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017587880.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTCCCCAT CTGCCTGGCA 
    GTAGCATGTG CATTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCG ACGATGCAAG GACACACAGG GCGGCTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCCTTT CTGCTCCTTC ACATCATTTT CCACATCACA
    CTGGCCAGCC TGGAAGCTCA GCACCGCATC GCACCCGCTT ACAACTGCTC AACTTGGGAA
    AAGACCTTCC GGCAGATCGG CTTCGAAAGC TTGAAGGGAG CTGATGCTGG CAATGGCATC
    AGGGTGTTTG TGCCTGATAT CGGGATGTTC ATTGCGAGCC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACGGAA GAAGTAGCCC AATTTAATTC CGAGTGCGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GACTCAGAAG AAGCGCTGAT TTATGAGGAG
    GACTTAGATG GGGAAGAAGG CATGGAAGGC GAGCTGGAAG AAAGCACAAA GTTAAAAATG
    TTTCGCAGGC TCGCCTCCGT CGCTTCCAAG CTTAAGGAGT TCATCGGCAA TATGATAACC
    ACGGCGGGCA AGGTGGTGGT GACCATCCTG TTGGGCTCCT CTGGCATGAT GCTGCCATCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT CTGGGTCTGT GCACCTGGTG GTCCTGGTGC
    CGAACTTTTG ACCCATTGCT GTTTGGCTGC CTCTGTGTCC TGTTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTTCAGTTCT TCCAAGAAGC AGTCCCACCC
    AATGACTACT ATGCAAGGTT GTTTGGAATC AAATCCGTTA TTCAAACAGA CTGCTCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTACAC GCTGGCCACG CTGATCCGCA TTTGGTTGCA AGAGCCTCTT
    GTGCAGGAGG AGATGTCGAA GGAAGAGGAG GGTGCACTGG ATGGCTGCTC CAATCAGAAC
    ACAGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    CTTTTATCCA TGACCCAAGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACTGTGAAC
    GGCAACCCTG TGGACTACCA CACCATCCAC CCAAGTCTGC CCATAGAGAA TGGCCCCGCC
    AAGACGGACT TGTATACTAC TCCTCAGTAC CGGTGGGAGC CCTCAGAGGA GTCCTCAGAA
    AAGAAAGAGG AGGAAGAGGA CAAGAGAGAA GACTCAGAAG GGGAAGGGAG CCAAGAGGAA
    AAACGGAGTG TGAGAATTCA TGCCATGGTC GCCGTGTTCC AGTTCATCAT GAAGCAGAGT
    TACATCTGTG CCCTCATCGC CATGATGGCA TGGAGCATCA CCTACCACAG CTGGCTGACA
    TTTGTGCTGT TGATCTGGTC GTGCACTCTC TGGATGATTC GCAACAGGAG GAAGTACGCC
    ATGATCAGCT CTCCGTTCAT GGTTGTCTAT GCCAATCTGC TGCTGGTACT GCAGTACATA
    TGGAGCTTTG AACTTCCAGA AATCAAGAAA GTCCCTGGAT TTCTAGAAAA GAAAGAGCCT
    GGAGAGCTTG CCTCAAAGAT CCTCTTCACC ATCACATTTT GGCTGCTCCT GAGGCAGCAT
    CTCACAGAGC AGAAGGCCCT TCAGGAAAAG GAAGCACTTC TGTCTGAAGT CAAAATTGGA
    AGTCAAGAAC TTGACGAAAA GGATGAGGAA CTCCAAGATA TACAAGTGGA AGGAGAGCCC
    ATAGAGAAGG AAGAAGAGGA GGAGGAAATA AAGGAAGACA GACATGAGGT AAAGAAGGAA
    GAGGAAGAGG AAGAGGAAGA TGACGATGAC CAGGACATCA TGAAGGTTCT GGGCAATCTG
    GTGGTGGCCC TCTTCATCAA GTATTGGATC TACGTCTGCG GGGGCATGTT CTTTTTTGTC
    AGCTTTGAGG GGAAAATCGT CATGTACAAA ATCATCTACA TGGTGCTGTT CCTGTTCTGT
    GTGGCCTTGT ACCAGGTGCA CTACGAGTGG TGGAGGAAGA TCCTGAAATA CTTCTGGATG
    TCGGTGGTCA TTTACACCAT GCTGGTGCTC ATCTTCATCT ACACTTACCA GTTTGAGAAC
    TTCCCAGGCC TGTGGCAAAA CATGACCGGG TTAAAGAAAG AGAAGCTTGA GGATCTGGGC
    CTGAAGCAGT TCACTGTGGC CGAACTGTTC ACACGAATAT TCATCCCCAC CTCCTTTCTG
    CTGGTCTGCA TCCTGCACCT CCACTATTTC CATGACCGGT TCCTGGAACT CACAGACCTC
    AAGTCCATCC CCAGCAAAGA AGACAACACC ATCTACAGCC ATGCCAAGGT CAATGGTCGC
    GTGTACCTAA TAATAAATAG CATCAAGAAA AAATTACCAA TCCACCAGAA TGAGTTAGCC
    CACCCTGAAG GAAGCCTCCC AGACCTAGCC ATTATGAACA TGACGGCCAG CCTCGATAAG
    CCTGAGAGGC AGAAGTTGGC AGAGCCTGGG GAGGAGAGGA CAGAAGAATG TGCCAAGAAG
    GCAGAGAAGG GTGAGGCTGG GAAAGACAGT GATGAGTCTG AGGAGGAGGA AGGTGAGGAA
    GAGGAATCCG AGGAGGAAGA ATCCTCAGAT CTCAGGAACA AGTGGCACCT CGTGATTGAC
    CGGCTCACTG TGCTCTTTCT AAAGTTCCTG GAGTATTTCC ACAAGTTGCA GGTGTTTATG
    TGGTGGATCT TGGAGCTGCA CATCATCAAA ATCGTCTCAT CGTACATTAT CTGGGTGACT
    GTCAAAGAGG TGTCTCTGTT CAACTATGTA TTTTTGATTT CTTGGGCATT TGCTCTGCCA
    TATGCCAAAC TCCGCCGTGT GGCTTCCAGT GTCTGCACTG TCTGGACGTG TGTGATCATC
    GTCTGCAAAA TGTTGTACCA GCTGCAAACC ATTAAGCCTG AGAACTTTTC TGTCAACTGC
    TCCTTGCCCA ATGAAAATCA AACCAACATA CCCCTTAATG AGTTGAACAA GTCTCTTCTC
    TACAGTGCCC CTGTTGATCC CACTGAGTGG GTTGGCCTGA GGAAGTCCTC CCCTTTACTC
    GTCTACCTGA GGAACAACCT CCTGATGTTG GCGATTTTGG CCTTCGAAGT CACTGTTTAC
    CGACATCAGG AGTACTATCG AGGCCGAAAC AACTTGACTG CTCCGGTGTC TAAAACTATC
    TTTCATGACA TCACAAGGTT ACATCTAGAT GATGGGCTTA TCAATTGTGC CAAATATTTT
    GTAAACTACT TCTTCTACAA GTTTGGCCTA GAGACCTGTT TCCTAATGTC AGTGAACGTA
    ATTGGACAGC GAATGGACTT CTACGCCATG ATTCATGCCT GTTGGCTGAT CGGTGTCTTG
    TACCGTCGCA GAAGAAAAGC CATTGCTGAG GTCTGGCCCA AGTACTGCTG CTTCCTGGCT
    TGCATCATCA CCTTCCAGTA CTTTGTCTGC ATAGGCATCC CTCCTGCCCC CTGCCGAGAT
    TACCCATGGA GGTTCAAGGG TGCCAACTTC AATGACAACA TCATCAAGTG GCTGTACTTC
    CCAGATTTCA TCGTGCGGCC CAACCCTGTG TTTCTTGTCT ATGACTTCAT GCTGCTCCTG
    TGTGCTTCCT TACAACGACA GATTTTTGAG GACGAAAACA AGGCAGCTGT GCGCATCATG
    GCCGGGGACA ATGTCGAGAT TTGCATGAAC CTTGACGCGG CCTCATTCAG CCAGCATAAC
    CCTGTACCAG ATTTCATTCA CTGCAGATCT TACTTGGACA TGTCCAAAGT GATCATCTTC
    AGCTACCTCT TCTGGTTCGT CCTCACCATC ATCTTCATTA CCGGAACGAC CAGGATCAGC
    ATCTTTTGCA TGGGTTACCT GGTGGCCTGT TTCTATTTCC TGCTCTTTGG AGGTGATCTG
    CTCCTGAAAC CCATCAAGAG CATCCTACGC TACTGGGACT GGCTGATTGC ATACAACGTG
    TTTGTGATCA CCATGAAAAA CATACTGTCA ATAGGAGCCT GTGGATACAT CGGTGCACTG
    GTGAGAAACA GCTGCTGGCT GATCCAAGCG TTCAGCCTGG CCTGCACGGT GAAAGGCTAT
    AAAATGCCTG AAGATGACTC CAGATGCAAG CTGCCCAGTG GGGAGGCAGG GATCATCTGG
    GACAGCATCT GCTTTGCGTT TCTCCTGCTA CAGAGGAGAG TCTTCATGAG TTACTATTTC
    CTGCATGTCG TGGCTGACAT TAAAGCATCC CAGATCCTGG CATCCAGAGG GGCCGAACTT
    TTCCAGGCTA CAATTGTGAA AGCTGTAAAG GCAAGAATTG AAGAAGAGAA AAAATCCATG
    GACCAGCTGA AGAGACAGAT GGATCGCATC AAAGCCCGGC AGCAAAAGTA CAAAAAGGGT
    AAGGAGAGGA TGCTGAGCTT GACCCAGGAG TCAGGGGAAG GCCAGGACAT CCAGAAAGTC
    TCAGAAGAAG ACGATGAAAG AGAAGCAGAC AAACAGAAAG CCAAGGGCAA AAAAAAGCAG
    TGGTGGCGGC CTTGGGTTGA TCATGCTTCC ATGGTCAGGA GTGGAGATTA TTATTTGTTT
    GAAACGGATA GTGAAGAGGA GGAAGAAGAG GAACTCAAAA AGGAGGATGA AGAACCACCA
    CGGAAGTCTG CATTCCAGTT CGTCTACCAA GCCTGGATTA CCGATCCTAA AACAGCACTC
    CGACAGAGGA GGAAAGAGAA GAAGAAAATG GCCAGAGAAG AGCAGAAGGG AAGACGCAAA
    GGATCTGGGG AGGGTCCAGT GGAATGGGAA GACAGAGAAG ATGAGCCAAT CAAAAAGAAA
    TCTGATGGAC CAGATAATAT CATCAAGAGG ATATTTAATA TCCTGAAGTT CACCTGGGTC
    CTGTTCCTGG CCACAGTGGA CAGTTTCACT ACTTGGCTCA ACTCCATCTC AAGAGAGCAT
    ATCGACATAT CCACAGTTCT GCGGATTGAA AGATGCATGC TGACCCGAGA GATAAAGAAG
    GGCAACGTTC CGACACGGGA AAGCATCCAC ATGTACTACC AGAACCACAT GATGAACCTT
    TCCAGAGAAT CCGGACTGGA CACAATCGAC GAGCACTCTG GCGCTGGTTC CAGGGCCCAG
    GCAGCACACA GGATGGATAG CTTAGATTCC CATGACAGTA TCTCCAGCTG TTACACTGAA
    GCAACCCTGC TGATCTCAAG GCAGTCCACC CTTGATGATT TAGATGGACA AGACCCTGTT
    CCTAAAACAA GCGAGCGTGC TCGGCCTAGG CTCCGTAAAG TGTTCAGCAT GGACATGTCC
    TCCTCATCAG CTGACAGTGG CAGTGTAGCA TCAAGCGAGC CCACACAGTG CACCATGCTG
    TATTCACGCC AAGGAACTAC GGAAACCATT GAGGAGGTAG AGGCTGAAGC TGAGGAGGAG
    GTGGTAGAGA GTCTGGAGCC TGAGCTCCAT GATGCCGAAG AGAAGGAATA TGCAGCTGAC
    TATGAAGCAG GGGTAGAGGA GGTCAGCCTC ACTCCAGATG AGGAGTTGCC TCAGTTCTCA
    ACAGATGACG GTGACGTGGA GGCCCCGCCC TCCTACAGCA AGGCTGTCAG CTTTGAGCAC
    CTCTCGTTTG CATCACAAGA TGACTCCGGG GGCAAGAACC ACATGGTGGT CAGCCCTGAC
    GACAGCCGCA CAGACAAGCT GGAGTCCAGT ATCTTACCGC CCCTGACTCA CGAGCTGACA
    GCCAGCGATC TGCTCATGAG CAAGATGTTC CGTGATGATG AGCTTGAAGA ATCTGAGAAA
    TTCTACGTGG ACCAGCCCCG GTTCTTGCTG CTATTCTATG CCATGTATAA CACTCTGGTA
    GCCCGCTCGG AAATGGTGTG CTATTTCGTG ATCATCCTGA ACCACATGAT CTCTGCCTCC
    ATCATTACTC TTCTGTTGCC CATCCTGATC TTCCTCTGGG CCATGCTGTC CGTCCCCAGG
    CCCAGCCGGC GCTTCTGGAT GATGGCCATC GTCTACACTG AGGTGGCGAT TGTAGTGAAA
    TATTTCTTCC AGTTTGGCTT CTTTCCTTGG AATAAGGACT TGGAAATTTA CAAGGACAGG
    CCCTACTTTC CACCAAACAT CATAGGGGTG GAAAAGAAAG AGGGCTATGT TCTTTACGAC
    CTCATTCAGC TGCTGGCTCT GTTCTTCCAT CGATCCATTC TGAAGTGTCA TGGCCTATGG
    GACGAGGATG ACATTGTTGA CAGTAACACA GACAAGGAAG GATCTGATGA TGAGCTGTCC
    CTCGGTCAGG GCAGAAGAGG CTCCTCAGAG TCACTGAAGT CCATCAACTT GGCTGCCTCT
    GTAGAGTCCG TCCATGTGAC CTTCCCAGAG CAGCCAGCAG CCATCCGGAG AAAACGCTCC
    TGCAGCAGCT CGCAGATCTC CCCACGGTCC AGCTTCTCTT CAAATAGGTC CAAAAGAGGC
    AGCACAAGCA CCCGAAACAG CAGCCAAAAA GGAAGCAGTG TTTTGAGCAT TAAACAAAAA
    AGCAAAAGAG AACTTTACAT GGAAAAGCTT CAAGAACATT TAATCAAAGC AAAGGCCTTT
    ACCATAAAGA AAACTCTACA AATCTATGTA CCCATTCGGC AATTCTTCTA TGACCTCATC
    CACCCAGACT ACAGTGCTGT GACTGACGTG TATGTGCTGA TGTTCCTGGC CGACACCGTG
    GACTTCATCA TCATCGTCTT TGGCTTCTGG GCTTTTGGGA AACACTCGGC AGCTGCAGAT
    ATCACCTCTT CCCTATCAGA AGACCAGGTC CCCGGGCCAT TCCTGGTGAT GGTTCTCATT
    CAGTTTGGAA CCATGGTGGT GGACCGTGCA CTCTACCTCA GGAAGACTGT GCTTGGCAAG
    GTCATTTTCC AGGTCATCCT CGTGTTTGGA ATTCACTTCT GGATGTTCTT CATCCTACCC
    AGTGTGACTG AGAGGAAATT CAGCCAGAAC CTGGTGGCAC AGCTCTGGTA CTTTGTGAAA
    TGTGTGTACT TTGGGTTGTC TGCCTACCAA ATCCGCTGTG GCTACCCAAC ACGTGTGCTC
    GGAAACTTCC TCACGAAGAG CTACAATTAT GTCAACCTCT TCTTGTTTCA AGGGTTTCGT
    CTCGTCCCAT TTCTGACAGA GCTGCGGGCT GTGATGGACT GGGTGTGGAC AGATACAACC
    TTGAGCCTCT CCAGCTGGAT TTGTGTGGAG GACATTTACG CACACATTTT CATCTTAAAG
    TGCTGGCGAG AGTCGGAAAA GAGATACCCT CAACCCAGGG GCCAGAAGAA GAAGAAAGCT
    GTGAAGTATG GCATGGGCGG AATGATCATT GTCCTGCTCA TCTGTATCGT CTGGTTTCCT
    CTGCTCTTCA TGTCTTTGAT CAAGTCTGTC GCAGGGGTTA TTAACCAACC CCTGGATGTG
    TCCGTCACAA TAACCCTGGG AGGCTATCAG CCTATCTTCA CAATGAGTGC CCAGCAAAGC
    CAGCTGAAAG TTATGGATGG TACAAAGTAT AACGAATTCA TAAAGTCTTT CGGCCCCAAC
    AGTGGCGCTA TGCAATTTCT GGAGAATTAT GAAAGGCAAG ACATAACAGT AGCGGAACTG
    GAAGGGAATT CGAATTCTTT GTGGACCATC AGCCCTCCCA GTAAGCAGAA AATGATACAG
    GAGCTCACCG ACCCCAATAG TTGCTTCTCT GTTGTTTTTT CATGGAGTAT CCAGAGAAAC
    ATGACTCTGG GTGCAAAGGC TGAAATAGCA ACGGACAAGC TTTCTTTTCC TCTCACGGAT
    GCGACACGAA GTAGCATAGC CAAAATGATT GCTGGCAATG ACACAGAAAG TTCCAACACA
    CCAGTGACAA TAGAAAAGAT CTACCCATAC TATGTGAAGG CACCCAGTGA CTCAAACTCA
    AAGCCTATAA AACAGCTTCT GTCAGAAAAC AATTTCATGA ATATCACCAT CATTTTGTTC
    AGAGACAATG TCACAAAGTC CAATAGTGAG TGGTGGGTTC TCAACCTGAC TGGAAGTAGG
    ATATTCAATC AGGGATCCCA AGCCTTGGAG CTGGTGGTCT TCAATGACAA AGTCAGCCCC
    CCAAGTCTGG GCTTCTTGGC TGGCTATGGC ATCATGGGAT TGTATGCATC CGTTGTCCTT
    GTAATTGGGA AGTTTGTTCG CGAATTCTTC AGTGGGATTT CTCACTCTAT CATGTTTGAA
    GAGCTTCCAA ATGTGGACAG AATTTTGAAG TTATGCACAG ATATATTTCT CGTACGGGAG
    ACAGGGGAAC TGGAGCTGGA GGAAGACCTC TATGCCAAAT TGATATTCCT GTACAGGTCG
    CCAGAAACCA TGATCAAGTG GACTAGAGAA AAAACAAATT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa54722
    Clone ID Related Accession (Same CDS sequence) XM_017587881.1 , XM_017601120.1
    Accession Version XM_017587881.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8433bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product piezo-type mechanosensitive ion channel component 2 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000086.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTCCCCAT CTGCCTGGCA 
    GTAGCATGTG CATTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCG ACGATGCAAG GACACACAGG GCGGCTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCCTTT CTGCTCCTTC ACATCATTTT CCACATCACA
    CTGGCCAGCC TGGAAGCTCA GCACCGCATC GCACCCGCTT ACAACTGCTC AACTTGGGAA
    AAGACCTTCC GGCAGATCGG CTTCGAAAGC TTGAAGGGAG CTGATGCTGG CAATGGCATC
    AGGGTGTTTG TGCCTGATAT CGGGATGTTC ATTGCGAGCC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACGGAA GAAGTAGCCC AATTTAATTC CGAGTGCGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GACTCAGAAG AAGCGCTGAT TTATGAGGAG
    GACTTAGATG GGGAAGAAGG CATGGAAGGC GAGCTGGAAG AAAGCACAAA GTTAAAAATG
    TTTCGCAGGC TCGCCTCCGT CGCTTCCAAG CTTAAGGAGT TCATCGGCAA TATGATAACC
    ACGGCGGGCA AGGTGGTGGT GACCATCCTG TTGGGCTCCT CTGGCATGAT GCTGCCATCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT CTGGGTCTGT GCACCTGGTG GTCCTGGTGC
    CGAACTTTTG ACCCATTGCT GTTTGGCTGC CTCTGTGTCC TGTTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTTCAGTTCT TCCAAGAAGC AGTCCCACCC
    AATGACTACT ATGCAAGGTT GTTTGGAATC AAATCCGTTA TTCAAACAGA CTGCTCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTACAC GCTGGCCACG CTGATCCGCA TTTGGTTGCA AGAGCCTCTT
    GTGCAGGAGG AGATGTCGAA GGAAGAGGAG GGTGCACTGG ATGGCTGCTC CAATCAGAAC
    ACAGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    CTTTTATCCA TGACCCAAGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACTGTGAAC
    GGCAACCCTG TGGACTACCA CACCATCCAC CCAAGTCTGC CCATAGAGAA TGGCCCCGCC
    AAGACGGACT TGTATACTAC TCCTCAGTAC CGGTGGGAGC CCTCAGAGGA GTCCTCAGAA
    AAGAAAGAGG AGGAAGAGGA CAAGAGAGAA GACTCAGAAG GGGAAGGGAG CCAAGAGGAA
    AAACGGAGTG TGAGAATTCA TGCCATGGTC GCCGTGTTCC AGTTCATCAT GAAGCAGAGT
    TACATCTGTG CCCTCATCGC CATGATGGCA TGGAGCATCA CCTACCACAG CTGGCTGACA
    TTTGTGCTGT TGATCTGGTC GTGCACTCTC TGGATGATTC GCAACAGGAG GAAGTACGCC
    ATGATCAGCT CTCCGTTCAT GGTTGTCTAT GCCAATCTGC TGCTGGTACT GCAGTACATA
    TGGAGCTTTG AACTTCCAGA AATCAAGAAA GTCCCTGGAT TTCTAGAAAA GAAAGAGCCT
    GGAGAGCTTG CCTCAAAGAT CCTCTTCACC ATCACATTTT GGCTGCTCCT GAGGCAGCAT
    CTCACAGAGC AGAAGGCCCT TCAGGAAAAG GAAGCACTTC TGTCTGAAGT CAAAATTGGA
    AGTCAAGAAC TTGACGAAAA GGATGAGGAA CTCCAAGATA TACAAGTGGA AGGAGAGCCC
    ATAGAGAAGG AAGAAGAGGA GGAGGAAATA AAGGAAGACA GACATGAGGT AAAGAAGGAA
    GAGGAAGAGG AAGAGGAAGA TGACGATGAC CAGGACATCA TGAAGGTTCT GGGCAATCTG
    GTGGTGGCCC TCTTCATCAA GTATTGGATC TACGTCTGCG GGGGCATGTT CTTTTTTGTC
    AGCTTTGAGG GGAAAATCGT CATGTACAAA ATCATCTACA TGGTGCTGTT CCTGTTCTGT
    GTGGCCTTGT ACCAGGTGCA CTACGAGTGG TGGAGGAAGA TCCTGAAATA CTTCTGGATG
    TCGGTGGTCA TTTACACCAT GCTGGTGCTC ATCTTCATCT ACACTTACCA GTTTGAGAAC
    TTCCCAGGCC TGTGGCAAAA CATGACCGGG TTAAAGAAAG AGAAGCTTGA GGATCTGGGC
    CTGAAGCAGT TCACTGTGGC CGAACTGTTC ACACGAATAT TCATCCCCAC CTCCTTTCTG
    CTGGTCTGCA TCCTGCACCT CCACTATTTC CATGACCGGT TCCTGGAACT CACAGACCTC
    AAGTCCATCC CCAGCAAAGA AGACAACACC ATCTACAGCC ATGCCAAGGT CAATGGTCGC
    GTGTACCTAA TAATAAATAG CATCAAGAAA AAATTACCAA TCCACCAGAA TGAGTTAGCC
    CACCCTGAAG GAAGCCTCCC AGACCTAGCC ATTATGAACA TGACGGCCAG CCTCGATAAG
    CCTGAGAGGC AGAAGTTGGC AGAGCCTGGG GAGGAGAGGA CAGAAGAATG TGCCAAGAAG
    GCAGAGAAGG GTGAGGCTGG GAAAGACAGT GATGAGTCTG AGGAGGAGGA AGGTGAGGAA
    GAGGAATCCG AGGAGGAAGA ATCCTCAGAT CTCAGGAACA AGTGGCACCT CGTGATTGAC
    CGGCTCACTG TGCTCTTTCT AAAGTTCCTG GAGTATTTCC ACAAGTTGCA GGTGTTTATG
    TGGTGGATCT TGGAGCTGCA CATCATCAAA ATCGTCTCAT CGTACATTAT CTGGGTGACT
    GTCAAAGAGG TGTCTCTGTT CAACTATGTA TTTTTGATTT CTTGGGCATT TGCTCTGCCA
    TATGCCAAAC TCCGCCGTGT GGCTTCCAGT GTCTGCACTG TCTGGACGTG TGTGATCATC
    GTCTGCAAAA TGTTGTACCA GCTGCAAACC ATTAAGCCTG AGAACTTTTC TGTCAACTGC
    TCCTTGCCCA ATGAAAATCA AACCAACATA CCCCTTAATG AGTTGAACAA GTCTCTTCTC
    TACAGTGCCC CTGTTGATCC CACTGAGTGG GTTGGCCTGA GGAAGTCCTC CCCTTTACTC
    GTCTACCTGA GGAACAACCT CCTGATGTTG GCGATTTTGG CCTTCGAAGT CACTGTTTAC
    CGACATCAGG AGTACTATCG AGGCCGAAAC AACTTGACTG CTCCGGTGTC TAAAACTATC
    TTTCATGACA TCACAAGGTT ACATCTAGAT GATGGGCTTA TCAATTGTGC CAAATATTTT
    GTAAACTACT TCTTCTACAA GTTTGGCCTA GAGACCTGTT TCCTAATGTC AGTGAACGTA
    ATTGGACAGC GAATGGACTT CTACGCCATG ATTCATGCCT GTTGGCTGAT CGGTGTCTTG
    TACCGTCGCA GAAGAAAAGC CATTGCTGAG GTCTGGCCCA AGTACTGCTG CTTCCTGGCT
    TGCATCATCA CCTTCCAGTA CTTTGTCTGC ATAGGCATCC CTCCTGCCCC CTGCCGAGAT
    TACCCATGGA GGTTCAAGGG TGCCAACTTC AATGACAACA TCATCAAGTG GCTGTACTTC
    CCAGATTTCA TCGTGCGGCC CAACCCTGTG TTTCTTGTCT ATGACTTCAT GCTGCTCCTG
    TGTGCTTCCT TACAACGACA GATTTTTGAG GACGAAAACA AGGCAGCTGT GCGCATCATG
    GCCGGGGACA ATGTCGAGAT TTGCATGAAC CTTGACGCGG CCTCATTCAG CCAGCATAAC
    CCTGTACCAG ATTTCATTCA CTGCAGATCT TACTTGGACA TGTCCAAAGT GATCATCTTC
    AGCTACCTCT TCTGGTTCGT CCTCACCATC ATCTTCATTA CCGGAACGAC CAGGATCAGC
    ATCTTTTGCA TGGGTTACCT GGTGGCCTGT TTCTATTTCC TGCTCTTTGG AGGTGATCTG
    CTCCTGAAAC CCATCAAGAG CATCCTACGC TACTGGGACT GGCTGATTGC ATACAACGTG
    TTTGTGATCA CCATGAAAAA CATACTGTCA ATAGGAGCCT GTGGATACAT CGGTGCACTG
    GTGAGAAACA GCTGCTGGCT GATCCAAGCG TTCAGCCTGG CCTGCACGGT GAAAGGCTAT
    AAAATGCCTG AAGATGACTC CAGATGCAAG CTGCCCAGTG GGGAGGCAGG GATCATCTGG
    GACAGCATCT GCTTTGCGTT TCTCCTGCTA CAGAGGAGAG TCTTCATGAG TTACTATTTC
    CTGCATGTCG TGGCTGACAT TAAAGCATCC CAGATCCTGG CATCCAGAGG GGCCGAACTT
    TTCCAGGCTA CAATTGTGAA AGCTGTAAAG GCAAGAATTG AAGAAGAGAA AAAATCCATG
    GACCAGCTGA AGAGACAGAT GGATCGCATC AAAGCCCGGC AGCAAAAGTA CAAAAAGGGT
    AAGGAGAGGA TGCTGAGCTT GACCCAGGAG TCAGGGGAAG GCCAGGACAT CCAGAAAGTC
    TCAGAAGAAG ACGATGAAAG AGAAGCAGAC AAACAGAAAG CCAAGGGCAA AAAAAAGCAG
    TGGTGGCGGC CTTGGGTTGA TCATGCTTCC ATGGTCAGGA GTGGAGATTA TTATTTGTTT
    GAAACGGATA GTGAAGAGGA GGAAGAAGAG GAACTCAAAA AGGAGGATGA AGAACCACCA
    CGGAAGTCTG CATTCCAGAG AGCTATTGGG AAGTTTGCCT CAGCCATTTT AGCCTTGCCA
    AAGTCTGTCA TTAAACTTCC CAAAACTATT CTTCAGTATT TAATCAGAGC TGCAAAGTTC
    GTCTACCAAG CCTGGATTAC CGATCCTAAA ACAGCACTCC GACAGAGGAG GAAAGAGAAG
    AAGAAAATGG CCAGAGAAGA GCAGAAGGGA AGACGCAAAG GATCTGGGGA GGGTCCAGTG
    GAATGGGAAG ACAGAGAAGA TGAGCCAATC AAAAAGAAAT CTGATGGACC AGATAATATC
    ATCAAGAGGA TATTTAATAT CCTGAAGTTC ACCTGGGTCC TGTTCCTGGC CACAGTGGAC
    AGTTTCACTA CTTGGCTCAA CTCCATCTCA AGAGAGCATA TCGACATATC CACAGTTCTG
    CGGATTGAAA GATGCATGCT GACCCGAGAG ATAAAGAAGG GCAACGTTCC GACACGGGAA
    AGCATCCACA TGTACTACCA GAACCACATG ATGAACCTTT CCAGAGAATC CGGACTGGAC
    ACAATCGACG AGCACTCTGG CGCTGGTTCC AGGGCCCAGG CAGCACACAG GATGGATAGC
    TTAGATTCCC ATGACAGTAT CTCCAGCGAG CCCACACAGT GCACCATGCT GTATTCACGC
    CAAGGAACTA CGGAAACCAT TGAGGAGGTA GAGGCTGAAG CTGAGGAGGA GGTGGTAGAG
    AGTCTGGAGC CTGAGCTCCA TGATGCCGAA GAGAAGGAAT ATGCAGCTGA CTATGAAGCA
    GGGGTAGAGG AGGTCAGCCT CACTCCAGAT GAGGAGTTGC CTCAGTTCTC AACAGATGAC
    GGTGACGTGG AGGCCCCGCC CTCCTACAGC AAGGCTGTCA GCTTTGAGCA CCTCTCGTTT
    GCATCACAAG ATGACTCCGG GGGCAAGAAC CACATGGTGG TCAGCCCTGA CGACAGCCGC
    ACAGACAAGC TGGAGTCCAG TATCTTACCG CCCCTGACTC ACGAGCTGAC AGCCAGCGAT
    CTGCTCATGA GCAAGATGTT CCGTGATGAT GAGCTTGAAG AATCTGAGAA ATTCTACGTG
    GACCAGCCCC GGTTCTTGCT GCTATTCTAT GCCATGTATA ACACTCTGGT AGCCCGCTCG
    GAAATGGTGT GCTATTTCGT GATCATCCTG AACCACATGA TCTCTGCCTC CATCATTACT
    CTTCTGTTGC CCATCCTGAT CTTCCTCTGG GCCATGCTGT CCGTCCCCAG GCCCAGCCGG
    CGCTTCTGGA TGATGGCCAT CGTCTACACT GAGGTGGCGA TTGTAGTGAA ATATTTCTTC
    CAGTTTGGCT TCTTTCCTTG GAATAAGGAC TTGGAAATTT ACAAGGACAG GCCCTACTTT
    CCACCAAACA TCATAGGGGT GGAAAAGAAA GAGGGCTATG TTCTTTACGA CCTCATTCAG
    CTGCTGGCTC TGTTCTTCCA TCGATCCATT CTGAAGTGTC ATGGCCTATG GGACGAGGAT
    GACATTGTTG ACAGTAACAC AGACAAGGAA GGATCTGATG ATGAGCTGTC CCTCGGTCAG
    GGCAGAAGAG GCTCCTCAGA GTCACTGAAG TCCATCAACT TGGCTGCCTC TGTAGAGTCC
    GTCCATGTGA CCTTCCCAGA GCAGCCAGCA GCCATCCGGA GAAAACGCTC CTGCAGCAGC
    TCGCAGATCT CCCCACGGTC CAGCTTCTCT TCAAATAGGT CCAAAAGAGG CAGCACAAGC
    ACCCGAAACA GCAGCCAAAA AGGAAGCAGT GTTTTGAGCA TTAAACAAAA AAGCAAAAGA
    GAACTTTACA TGGAAAAGCT TCAAGAACAT TTAATCAAAG CAAAGGCCTT TACCATAAAG
    AAAACTCTAC AAATCTATGT ACCCATTCGG CAATTCTTCT ATGACCTCAT CCACCCAGAC
    TACAGTGCTG TGACTGACGT GTATGTGCTG ATGTTCCTGG CCGACACCGT GGACTTCATC
    ATCATCGTCT TTGGCTTCTG GGCTTTTGGG AAACACTCGG CAGCTGCAGA TATCACCTCT
    TCCCTATCAG AAGACCAGGT CCCCGGGCCA TTCCTGGTGA TGGTTCTCAT TCAGTTTGGA
    ACCATGGTGG TGGACCGTGC ACTCTACCTC AGGAAGACTG TGCTTGGCAA GGTCATTTTC
    CAGGTCATCC TCGTGTTTGG AATTCACTTC TGGATGTTCT TCATCCTACC CAGTGTGACT
    GAGAGGAAAT TCAGCCAGAA CCTGGTGGCA CAGCTCTGGT ACTTTGTGAA ATGTGTGTAC
    TTTGGGTTGT CTGCCTACCA AATCCGCTGT GGCTACCCAA CACGTGTGCT CGGAAACTTC
    CTCACGAAGA GCTACAATTA TGTCAACCTC TTCTTGTTTC AAGGGTTTCG TCTCGTCCCA
    TTTCTGACAG AGCTGCGGGC TGTGATGGAC TGGGTGTGGA CAGATACAAC CTTGAGCCTC
    TCCAGCTGGA TTTGTGTGGA GGACATTTAC GCACACATTT TCATCTTAAA GTGCTGGCGA
    GAGTCGGAAA AGAGATACCC TCAACCCAGG GGCCAGAAGA AGAAGAAAGC TGTGAAGTAT
    GGCATGGGCG GAATGATCAT TGTCCTGCTC ATCTGTATCG TCTGGTTTCC TCTGCTCTTC
    ATGTCTTTGA TCAAGTCTGT CGCAGGGGTT ATTAACCAAC CCCTGGATGT GTCCGTCACA
    ATAACCCTGG GAGGCTATCA GCCTATCTTC ACAATGAGTG CCCAGCAAAG CCAGCTGAAA
    GTTATGGATG GTACAAAGTA TAACGAATTC ATAAAGTCTT TCGGCCCCAA CAGTGGCGCT
    ATGCAATTTC TGGAGAATTA TGAAAGGCAA GACATAACAG TAGCGGAACT GGAAGGGAAT
    TCGAATTCTT TGTGGACCAT CAGCCCTCCC AGTAAGCAGA AAATGATACA GGAGCTCACC
    GACCCCAATA GTTGCTTCTC TGTTGTTTTT TCATGGAGTA TCCAGAGAAA CATGACTCTG
    GGTGCAAAGG CTGAAATAGC AACGGACAAG CTTTCTTTTC CTCTCACGGA TGCGACACGA
    AGTAGCATAG CCAAAATGAT TGCTGGCAAT GACACAGAAA GTTCCAACAC ACCAGTGACA
    ATAGAAAAGA TCTACCCATA CTATGTGAAG GCACCCAGTG ACTCAAACTC AAAGCCTATA
    AAACAGCTTC TGTCAGAAAA CAATTTCATG AATATCACCA TCATTTTGTT CAGAGACAAT
    GTCACAAAGT CCAATAGTGA GTGGTGGGTT CTCAACCTGA CTGGAAGTAG GATATTCAAT
    CAGGGATCCC AAGCCTTGGA GCTGGTGGTC TTCAATGACA AAGTCAGCCC CCCAAGTCTG
    GGCTTCTTGG CTGGCTATGG CATCATGGGA TTGTATGCAT CCGTTGTCCT TGTAATTGGG
    AAGTTTGTTC GCGAATTCTT CAGTGGGATT TCTCACTCTA TCATGTTTGA AGAGCTTCCA
    AATGTGGACA GAATTTTGAA GTTATGCACA GATATATTTC TCGTACGGGA GACAGGGGAA
    CTGGAGCTGG AGGAAGACCT CTATGCCAAA TTGATATTCC TGTACAGGTC GCCAGAAACC
    ATGATCAAGT GGACTAGAGA AAAAACAAAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017443370.1
    CDS940..9372
    Translation

    Target ORF information:

    RefSeq Version XM_017587881.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017587881.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTCCCCAT CTGCCTGGCA 
    GTAGCATGTG CATTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCG ACGATGCAAG GACACACAGG GCGGCTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCCTTT CTGCTCCTTC ACATCATTTT CCACATCACA
    CTGGCCAGCC TGGAAGCTCA GCACCGCATC GCACCCGCTT ACAACTGCTC AACTTGGGAA
    AAGACCTTCC GGCAGATCGG CTTCGAAAGC TTGAAGGGAG CTGATGCTGG CAATGGCATC
    AGGGTGTTTG TGCCTGATAT CGGGATGTTC ATTGCGAGCC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACGGAA GAAGTAGCCC AATTTAATTC CGAGTGCGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GACTCAGAAG AAGCGCTGAT TTATGAGGAG
    GACTTAGATG GGGAAGAAGG CATGGAAGGC GAGCTGGAAG AAAGCACAAA GTTAAAAATG
    TTTCGCAGGC TCGCCTCCGT CGCTTCCAAG CTTAAGGAGT TCATCGGCAA TATGATAACC
    ACGGCGGGCA AGGTGGTGGT GACCATCCTG TTGGGCTCCT CTGGCATGAT GCTGCCATCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT CTGGGTCTGT GCACCTGGTG GTCCTGGTGC
    CGAACTTTTG ACCCATTGCT GTTTGGCTGC CTCTGTGTCC TGTTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTTCAGTTCT TCCAAGAAGC AGTCCCACCC
    AATGACTACT ATGCAAGGTT GTTTGGAATC AAATCCGTTA TTCAAACAGA CTGCTCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTACAC GCTGGCCACG CTGATCCGCA TTTGGTTGCA AGAGCCTCTT
    GTGCAGGAGG AGATGTCGAA GGAAGAGGAG GGTGCACTGG ATGGCTGCTC CAATCAGAAC
    ACAGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    CTTTTATCCA TGACCCAAGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACTGTGAAC
    GGCAACCCTG TGGACTACCA CACCATCCAC CCAAGTCTGC CCATAGAGAA TGGCCCCGCC
    AAGACGGACT TGTATACTAC TCCTCAGTAC CGGTGGGAGC CCTCAGAGGA GTCCTCAGAA
    AAGAAAGAGG AGGAAGAGGA CAAGAGAGAA GACTCAGAAG GGGAAGGGAG CCAAGAGGAA
    AAACGGAGTG TGAGAATTCA TGCCATGGTC GCCGTGTTCC AGTTCATCAT GAAGCAGAGT
    TACATCTGTG CCCTCATCGC CATGATGGCA TGGAGCATCA CCTACCACAG CTGGCTGACA
    TTTGTGCTGT TGATCTGGTC GTGCACTCTC TGGATGATTC GCAACAGGAG GAAGTACGCC
    ATGATCAGCT CTCCGTTCAT GGTTGTCTAT GCCAATCTGC TGCTGGTACT GCAGTACATA
    TGGAGCTTTG AACTTCCAGA AATCAAGAAA GTCCCTGGAT TTCTAGAAAA GAAAGAGCCT
    GGAGAGCTTG CCTCAAAGAT CCTCTTCACC ATCACATTTT GGCTGCTCCT GAGGCAGCAT
    CTCACAGAGC AGAAGGCCCT TCAGGAAAAG GAAGCACTTC TGTCTGAAGT CAAAATTGGA
    AGTCAAGAAC TTGACGAAAA GGATGAGGAA CTCCAAGATA TACAAGTGGA AGGAGAGCCC
    ATAGAGAAGG AAGAAGAGGA GGAGGAAATA AAGGAAGACA GACATGAGGT AAAGAAGGAA
    GAGGAAGAGG AAGAGGAAGA TGACGATGAC CAGGACATCA TGAAGGTTCT GGGCAATCTG
    GTGGTGGCCC TCTTCATCAA GTATTGGATC TACGTCTGCG GGGGCATGTT CTTTTTTGTC
    AGCTTTGAGG GGAAAATCGT CATGTACAAA ATCATCTACA TGGTGCTGTT CCTGTTCTGT
    GTGGCCTTGT ACCAGGTGCA CTACGAGTGG TGGAGGAAGA TCCTGAAATA CTTCTGGATG
    TCGGTGGTCA TTTACACCAT GCTGGTGCTC ATCTTCATCT ACACTTACCA GTTTGAGAAC
    TTCCCAGGCC TGTGGCAAAA CATGACCGGG TTAAAGAAAG AGAAGCTTGA GGATCTGGGC
    CTGAAGCAGT TCACTGTGGC CGAACTGTTC ACACGAATAT TCATCCCCAC CTCCTTTCTG
    CTGGTCTGCA TCCTGCACCT CCACTATTTC CATGACCGGT TCCTGGAACT CACAGACCTC
    AAGTCCATCC CCAGCAAAGA AGACAACACC ATCTACAGCC ATGCCAAGGT CAATGGTCGC
    GTGTACCTAA TAATAAATAG CATCAAGAAA AAATTACCAA TCCACCAGAA TGAGTTAGCC
    CACCCTGAAG GAAGCCTCCC AGACCTAGCC ATTATGAACA TGACGGCCAG CCTCGATAAG
    CCTGAGAGGC AGAAGTTGGC AGAGCCTGGG GAGGAGAGGA CAGAAGAATG TGCCAAGAAG
    GCAGAGAAGG GTGAGGCTGG GAAAGACAGT GATGAGTCTG AGGAGGAGGA AGGTGAGGAA
    GAGGAATCCG AGGAGGAAGA ATCCTCAGAT CTCAGGAACA AGTGGCACCT CGTGATTGAC
    CGGCTCACTG TGCTCTTTCT AAAGTTCCTG GAGTATTTCC ACAAGTTGCA GGTGTTTATG
    TGGTGGATCT TGGAGCTGCA CATCATCAAA ATCGTCTCAT CGTACATTAT CTGGGTGACT
    GTCAAAGAGG TGTCTCTGTT CAACTATGTA TTTTTGATTT CTTGGGCATT TGCTCTGCCA
    TATGCCAAAC TCCGCCGTGT GGCTTCCAGT GTCTGCACTG TCTGGACGTG TGTGATCATC
    GTCTGCAAAA TGTTGTACCA GCTGCAAACC ATTAAGCCTG AGAACTTTTC TGTCAACTGC
    TCCTTGCCCA ATGAAAATCA AACCAACATA CCCCTTAATG AGTTGAACAA GTCTCTTCTC
    TACAGTGCCC CTGTTGATCC CACTGAGTGG GTTGGCCTGA GGAAGTCCTC CCCTTTACTC
    GTCTACCTGA GGAACAACCT CCTGATGTTG GCGATTTTGG CCTTCGAAGT CACTGTTTAC
    CGACATCAGG AGTACTATCG AGGCCGAAAC AACTTGACTG CTCCGGTGTC TAAAACTATC
    TTTCATGACA TCACAAGGTT ACATCTAGAT GATGGGCTTA TCAATTGTGC CAAATATTTT
    GTAAACTACT TCTTCTACAA GTTTGGCCTA GAGACCTGTT TCCTAATGTC AGTGAACGTA
    ATTGGACAGC GAATGGACTT CTACGCCATG ATTCATGCCT GTTGGCTGAT CGGTGTCTTG
    TACCGTCGCA GAAGAAAAGC CATTGCTGAG GTCTGGCCCA AGTACTGCTG CTTCCTGGCT
    TGCATCATCA CCTTCCAGTA CTTTGTCTGC ATAGGCATCC CTCCTGCCCC CTGCCGAGAT
    TACCCATGGA GGTTCAAGGG TGCCAACTTC AATGACAACA TCATCAAGTG GCTGTACTTC
    CCAGATTTCA TCGTGCGGCC CAACCCTGTG TTTCTTGTCT ATGACTTCAT GCTGCTCCTG
    TGTGCTTCCT TACAACGACA GATTTTTGAG GACGAAAACA AGGCAGCTGT GCGCATCATG
    GCCGGGGACA ATGTCGAGAT TTGCATGAAC CTTGACGCGG CCTCATTCAG CCAGCATAAC
    CCTGTACCAG ATTTCATTCA CTGCAGATCT TACTTGGACA TGTCCAAAGT GATCATCTTC
    AGCTACCTCT TCTGGTTCGT CCTCACCATC ATCTTCATTA CCGGAACGAC CAGGATCAGC
    ATCTTTTGCA TGGGTTACCT GGTGGCCTGT TTCTATTTCC TGCTCTTTGG AGGTGATCTG
    CTCCTGAAAC CCATCAAGAG CATCCTACGC TACTGGGACT GGCTGATTGC ATACAACGTG
    TTTGTGATCA CCATGAAAAA CATACTGTCA ATAGGAGCCT GTGGATACAT CGGTGCACTG
    GTGAGAAACA GCTGCTGGCT GATCCAAGCG TTCAGCCTGG CCTGCACGGT GAAAGGCTAT
    AAAATGCCTG AAGATGACTC CAGATGCAAG CTGCCCAGTG GGGAGGCAGG GATCATCTGG
    GACAGCATCT GCTTTGCGTT TCTCCTGCTA CAGAGGAGAG TCTTCATGAG TTACTATTTC
    CTGCATGTCG TGGCTGACAT TAAAGCATCC CAGATCCTGG CATCCAGAGG GGCCGAACTT
    TTCCAGGCTA CAATTGTGAA AGCTGTAAAG GCAAGAATTG AAGAAGAGAA AAAATCCATG
    GACCAGCTGA AGAGACAGAT GGATCGCATC AAAGCCCGGC AGCAAAAGTA CAAAAAGGGT
    AAGGAGAGGA TGCTGAGCTT GACCCAGGAG TCAGGGGAAG GCCAGGACAT CCAGAAAGTC
    TCAGAAGAAG ACGATGAAAG AGAAGCAGAC AAACAGAAAG CCAAGGGCAA AAAAAAGCAG
    TGGTGGCGGC CTTGGGTTGA TCATGCTTCC ATGGTCAGGA GTGGAGATTA TTATTTGTTT
    GAAACGGATA GTGAAGAGGA GGAAGAAGAG GAACTCAAAA AGGAGGATGA AGAACCACCA
    CGGAAGTCTG CATTCCAGAG AGCTATTGGG AAGTTTGCCT CAGCCATTTT AGCCTTGCCA
    AAGTCTGTCA TTAAACTTCC CAAAACTATT CTTCAGTATT TAATCAGAGC TGCAAAGTTC
    GTCTACCAAG CCTGGATTAC CGATCCTAAA ACAGCACTCC GACAGAGGAG GAAAGAGAAG
    AAGAAAATGG CCAGAGAAGA GCAGAAGGGA AGACGCAAAG GATCTGGGGA GGGTCCAGTG
    GAATGGGAAG ACAGAGAAGA TGAGCCAATC AAAAAGAAAT CTGATGGACC AGATAATATC
    ATCAAGAGGA TATTTAATAT CCTGAAGTTC ACCTGGGTCC TGTTCCTGGC CACAGTGGAC
    AGTTTCACTA CTTGGCTCAA CTCCATCTCA AGAGAGCATA TCGACATATC CACAGTTCTG
    CGGATTGAAA GATGCATGCT GACCCGAGAG ATAAAGAAGG GCAACGTTCC GACACGGGAA
    AGCATCCACA TGTACTACCA GAACCACATG ATGAACCTTT CCAGAGAATC CGGACTGGAC
    ACAATCGACG AGCACTCTGG CGCTGGTTCC AGGGCCCAGG CAGCACACAG GATGGATAGC
    TTAGATTCCC ATGACAGTAT CTCCAGCGAG CCCACACAGT GCACCATGCT GTATTCACGC
    CAAGGAACTA CGGAAACCAT TGAGGAGGTA GAGGCTGAAG CTGAGGAGGA GGTGGTAGAG
    AGTCTGGAGC CTGAGCTCCA TGATGCCGAA GAGAAGGAAT ATGCAGCTGA CTATGAAGCA
    GGGGTAGAGG AGGTCAGCCT CACTCCAGAT GAGGAGTTGC CTCAGTTCTC AACAGATGAC
    GGTGACGTGG AGGCCCCGCC CTCCTACAGC AAGGCTGTCA GCTTTGAGCA CCTCTCGTTT
    GCATCACAAG ATGACTCCGG GGGCAAGAAC CACATGGTGG TCAGCCCTGA CGACAGCCGC
    ACAGACAAGC TGGAGTCCAG TATCTTACCG CCCCTGACTC ACGAGCTGAC AGCCAGCGAT
    CTGCTCATGA GCAAGATGTT CCGTGATGAT GAGCTTGAAG AATCTGAGAA ATTCTACGTG
    GACCAGCCCC GGTTCTTGCT GCTATTCTAT GCCATGTATA ACACTCTGGT AGCCCGCTCG
    GAAATGGTGT GCTATTTCGT GATCATCCTG AACCACATGA TCTCTGCCTC CATCATTACT
    CTTCTGTTGC CCATCCTGAT CTTCCTCTGG GCCATGCTGT CCGTCCCCAG GCCCAGCCGG
    CGCTTCTGGA TGATGGCCAT CGTCTACACT GAGGTGGCGA TTGTAGTGAA ATATTTCTTC
    CAGTTTGGCT TCTTTCCTTG GAATAAGGAC TTGGAAATTT ACAAGGACAG GCCCTACTTT
    CCACCAAACA TCATAGGGGT GGAAAAGAAA GAGGGCTATG TTCTTTACGA CCTCATTCAG
    CTGCTGGCTC TGTTCTTCCA TCGATCCATT CTGAAGTGTC ATGGCCTATG GGACGAGGAT
    GACATTGTTG ACAGTAACAC AGACAAGGAA GGATCTGATG ATGAGCTGTC CCTCGGTCAG
    GGCAGAAGAG GCTCCTCAGA GTCACTGAAG TCCATCAACT TGGCTGCCTC TGTAGAGTCC
    GTCCATGTGA CCTTCCCAGA GCAGCCAGCA GCCATCCGGA GAAAACGCTC CTGCAGCAGC
    TCGCAGATCT CCCCACGGTC CAGCTTCTCT TCAAATAGGT CCAAAAGAGG CAGCACAAGC
    ACCCGAAACA GCAGCCAAAA AGGAAGCAGT GTTTTGAGCA TTAAACAAAA AAGCAAAAGA
    GAACTTTACA TGGAAAAGCT TCAAGAACAT TTAATCAAAG CAAAGGCCTT TACCATAAAG
    AAAACTCTAC AAATCTATGT ACCCATTCGG CAATTCTTCT ATGACCTCAT CCACCCAGAC
    TACAGTGCTG TGACTGACGT GTATGTGCTG ATGTTCCTGG CCGACACCGT GGACTTCATC
    ATCATCGTCT TTGGCTTCTG GGCTTTTGGG AAACACTCGG CAGCTGCAGA TATCACCTCT
    TCCCTATCAG AAGACCAGGT CCCCGGGCCA TTCCTGGTGA TGGTTCTCAT TCAGTTTGGA
    ACCATGGTGG TGGACCGTGC ACTCTACCTC AGGAAGACTG TGCTTGGCAA GGTCATTTTC
    CAGGTCATCC TCGTGTTTGG AATTCACTTC TGGATGTTCT TCATCCTACC CAGTGTGACT
    GAGAGGAAAT TCAGCCAGAA CCTGGTGGCA CAGCTCTGGT ACTTTGTGAA ATGTGTGTAC
    TTTGGGTTGT CTGCCTACCA AATCCGCTGT GGCTACCCAA CACGTGTGCT CGGAAACTTC
    CTCACGAAGA GCTACAATTA TGTCAACCTC TTCTTGTTTC AAGGGTTTCG TCTCGTCCCA
    TTTCTGACAG AGCTGCGGGC TGTGATGGAC TGGGTGTGGA CAGATACAAC CTTGAGCCTC
    TCCAGCTGGA TTTGTGTGGA GGACATTTAC GCACACATTT TCATCTTAAA GTGCTGGCGA
    GAGTCGGAAA AGAGATACCC TCAACCCAGG GGCCAGAAGA AGAAGAAAGC TGTGAAGTAT
    GGCATGGGCG GAATGATCAT TGTCCTGCTC ATCTGTATCG TCTGGTTTCC TCTGCTCTTC
    ATGTCTTTGA TCAAGTCTGT CGCAGGGGTT ATTAACCAAC CCCTGGATGT GTCCGTCACA
    ATAACCCTGG GAGGCTATCA GCCTATCTTC ACAATGAGTG CCCAGCAAAG CCAGCTGAAA
    GTTATGGATG GTACAAAGTA TAACGAATTC ATAAAGTCTT TCGGCCCCAA CAGTGGCGCT
    ATGCAATTTC TGGAGAATTA TGAAAGGCAA GACATAACAG TAGCGGAACT GGAAGGGAAT
    TCGAATTCTT TGTGGACCAT CAGCCCTCCC AGTAAGCAGA AAATGATACA GGAGCTCACC
    GACCCCAATA GTTGCTTCTC TGTTGTTTTT TCATGGAGTA TCCAGAGAAA CATGACTCTG
    GGTGCAAAGG CTGAAATAGC AACGGACAAG CTTTCTTTTC CTCTCACGGA TGCGACACGA
    AGTAGCATAG CCAAAATGAT TGCTGGCAAT GACACAGAAA GTTCCAACAC ACCAGTGACA
    ATAGAAAAGA TCTACCCATA CTATGTGAAG GCACCCAGTG ACTCAAACTC AAAGCCTATA
    AAACAGCTTC TGTCAGAAAA CAATTTCATG AATATCACCA TCATTTTGTT CAGAGACAAT
    GTCACAAAGT CCAATAGTGA GTGGTGGGTT CTCAACCTGA CTGGAAGTAG GATATTCAAT
    CAGGGATCCC AAGCCTTGGA GCTGGTGGTC TTCAATGACA AAGTCAGCCC CCCAAGTCTG
    GGCTTCTTGG CTGGCTATGG CATCATGGGA TTGTATGCAT CCGTTGTCCT TGTAATTGGG
    AAGTTTGTTC GCGAATTCTT CAGTGGGATT TCTCACTCTA TCATGTTTGA AGAGCTTCCA
    AATGTGGACA GAATTTTGAA GTTATGCACA GATATATTTC TCGTACGGGA GACAGGGGAA
    CTGGAGCTGG AGGAAGACCT CTATGCCAAA TTGATATTCC TGTACAGGTC GCCAGAAACC
    ATGATCAAGT GGACTAGAGA AAAAACAAAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa54722
    Clone ID Related Accession (Same CDS sequence) XM_017587881.1 , XM_017601120.1
    Accession Version XM_017601120.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8433bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product piezo-type mechanosensitive ion channel component 2 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005117.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTCCCCAT CTGCCTGGCA 
    GTAGCATGTG CATTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCG ACGATGCAAG GACACACAGG GCGGCTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCCTTT CTGCTCCTTC ACATCATTTT CCACATCACA
    CTGGCCAGCC TGGAAGCTCA GCACCGCATC GCACCCGCTT ACAACTGCTC AACTTGGGAA
    AAGACCTTCC GGCAGATCGG CTTCGAAAGC TTGAAGGGAG CTGATGCTGG CAATGGCATC
    AGGGTGTTTG TGCCTGATAT CGGGATGTTC ATTGCGAGCC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACGGAA GAAGTAGCCC AATTTAATTC CGAGTGCGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GACTCAGAAG AAGCGCTGAT TTATGAGGAG
    GACTTAGATG GGGAAGAAGG CATGGAAGGC GAGCTGGAAG AAAGCACAAA GTTAAAAATG
    TTTCGCAGGC TCGCCTCCGT CGCTTCCAAG CTTAAGGAGT TCATCGGCAA TATGATAACC
    ACGGCGGGCA AGGTGGTGGT GACCATCCTG TTGGGCTCCT CTGGCATGAT GCTGCCATCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT CTGGGTCTGT GCACCTGGTG GTCCTGGTGC
    CGAACTTTTG ACCCATTGCT GTTTGGCTGC CTCTGTGTCC TGTTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTTCAGTTCT TCCAAGAAGC AGTCCCACCC
    AATGACTACT ATGCAAGGTT GTTTGGAATC AAATCCGTTA TTCAAACAGA CTGCTCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTACAC GCTGGCCACG CTGATCCGCA TTTGGTTGCA AGAGCCTCTT
    GTGCAGGAGG AGATGTCGAA GGAAGAGGAG GGTGCACTGG ATGGCTGCTC CAATCAGAAC
    ACAGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    CTTTTATCCA TGACCCAAGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACTGTGAAC
    GGCAACCCTG TGGACTACCA CACCATCCAC CCAAGTCTGC CCATAGAGAA TGGCCCCGCC
    AAGACGGACT TGTATACTAC TCCTCAGTAC CGGTGGGAGC CCTCAGAGGA GTCCTCAGAA
    AAGAAAGAGG AGGAAGAGGA CAAGAGAGAA GACTCAGAAG GGGAAGGGAG CCAAGAGGAA
    AAACGGAGTG TGAGAATTCA TGCCATGGTC GCCGTGTTCC AGTTCATCAT GAAGCAGAGT
    TACATCTGTG CCCTCATCGC CATGATGGCA TGGAGCATCA CCTACCACAG CTGGCTGACA
    TTTGTGCTGT TGATCTGGTC GTGCACTCTC TGGATGATTC GCAACAGGAG GAAGTACGCC
    ATGATCAGCT CTCCGTTCAT GGTTGTCTAT GCCAATCTGC TGCTGGTACT GCAGTACATA
    TGGAGCTTTG AACTTCCAGA AATCAAGAAA GTCCCTGGAT TTCTAGAAAA GAAAGAGCCT
    GGAGAGCTTG CCTCAAAGAT CCTCTTCACC ATCACATTTT GGCTGCTCCT GAGGCAGCAT
    CTCACAGAGC AGAAGGCCCT TCAGGAAAAG GAAGCACTTC TGTCTGAAGT CAAAATTGGA
    AGTCAAGAAC TTGACGAAAA GGATGAGGAA CTCCAAGATA TACAAGTGGA AGGAGAGCCC
    ATAGAGAAGG AAGAAGAGGA GGAGGAAATA AAGGAAGACA GACATGAGGT AAAGAAGGAA
    GAGGAAGAGG AAGAGGAAGA TGACGATGAC CAGGACATCA TGAAGGTTCT GGGCAATCTG
    GTGGTGGCCC TCTTCATCAA GTATTGGATC TACGTCTGCG GGGGCATGTT CTTTTTTGTC
    AGCTTTGAGG GGAAAATCGT CATGTACAAA ATCATCTACA TGGTGCTGTT CCTGTTCTGT
    GTGGCCTTGT ACCAGGTGCA CTACGAGTGG TGGAGGAAGA TCCTGAAATA CTTCTGGATG
    TCGGTGGTCA TTTACACCAT GCTGGTGCTC ATCTTCATCT ACACTTACCA GTTTGAGAAC
    TTCCCAGGCC TGTGGCAAAA CATGACCGGG TTAAAGAAAG AGAAGCTTGA GGATCTGGGC
    CTGAAGCAGT TCACTGTGGC CGAACTGTTC ACACGAATAT TCATCCCCAC CTCCTTTCTG
    CTGGTCTGCA TCCTGCACCT CCACTATTTC CATGACCGGT TCCTGGAACT CACAGACCTC
    AAGTCCATCC CCAGCAAAGA AGACAACACC ATCTACAGCC ATGCCAAGGT CAATGGTCGC
    GTGTACCTAA TAATAAATAG CATCAAGAAA AAATTACCAA TCCACCAGAA TGAGTTAGCC
    CACCCTGAAG GAAGCCTCCC AGACCTAGCC ATTATGAACA TGACGGCCAG CCTCGATAAG
    CCTGAGAGGC AGAAGTTGGC AGAGCCTGGG GAGGAGAGGA CAGAAGAATG TGCCAAGAAG
    GCAGAGAAGG GTGAGGCTGG GAAAGACAGT GATGAGTCTG AGGAGGAGGA AGGTGAGGAA
    GAGGAATCCG AGGAGGAAGA ATCCTCAGAT CTCAGGAACA AGTGGCACCT CGTGATTGAC
    CGGCTCACTG TGCTCTTTCT AAAGTTCCTG GAGTATTTCC ACAAGTTGCA GGTGTTTATG
    TGGTGGATCT TGGAGCTGCA CATCATCAAA ATCGTCTCAT CGTACATTAT CTGGGTGACT
    GTCAAAGAGG TGTCTCTGTT CAACTATGTA TTTTTGATTT CTTGGGCATT TGCTCTGCCA
    TATGCCAAAC TCCGCCGTGT GGCTTCCAGT GTCTGCACTG TCTGGACGTG TGTGATCATC
    GTCTGCAAAA TGTTGTACCA GCTGCAAACC ATTAAGCCTG AGAACTTTTC TGTCAACTGC
    TCCTTGCCCA ATGAAAATCA AACCAACATA CCCCTTAATG AGTTGAACAA GTCTCTTCTC
    TACAGTGCCC CTGTTGATCC CACTGAGTGG GTTGGCCTGA GGAAGTCCTC CCCTTTACTC
    GTCTACCTGA GGAACAACCT CCTGATGTTG GCGATTTTGG CCTTCGAAGT CACTGTTTAC
    CGACATCAGG AGTACTATCG AGGCCGAAAC AACTTGACTG CTCCGGTGTC TAAAACTATC
    TTTCATGACA TCACAAGGTT ACATCTAGAT GATGGGCTTA TCAATTGTGC CAAATATTTT
    GTAAACTACT TCTTCTACAA GTTTGGCCTA GAGACCTGTT TCCTAATGTC AGTGAACGTA
    ATTGGACAGC GAATGGACTT CTACGCCATG ATTCATGCCT GTTGGCTGAT CGGTGTCTTG
    TACCGTCGCA GAAGAAAAGC CATTGCTGAG GTCTGGCCCA AGTACTGCTG CTTCCTGGCT
    TGCATCATCA CCTTCCAGTA CTTTGTCTGC ATAGGCATCC CTCCTGCCCC CTGCCGAGAT
    TACCCATGGA GGTTCAAGGG TGCCAACTTC AATGACAACA TCATCAAGTG GCTGTACTTC
    CCAGATTTCA TCGTGCGGCC CAACCCTGTG TTTCTTGTCT ATGACTTCAT GCTGCTCCTG
    TGTGCTTCCT TACAACGACA GATTTTTGAG GACGAAAACA AGGCAGCTGT GCGCATCATG
    GCCGGGGACA ATGTCGAGAT TTGCATGAAC CTTGACGCGG CCTCATTCAG CCAGCATAAC
    CCTGTACCAG ATTTCATTCA CTGCAGATCT TACTTGGACA TGTCCAAAGT GATCATCTTC
    AGCTACCTCT TCTGGTTCGT CCTCACCATC ATCTTCATTA CCGGAACGAC CAGGATCAGC
    ATCTTTTGCA TGGGTTACCT GGTGGCCTGT TTCTATTTCC TGCTCTTTGG AGGTGATCTG
    CTCCTGAAAC CCATCAAGAG CATCCTACGC TACTGGGACT GGCTGATTGC ATACAACGTG
    TTTGTGATCA CCATGAAAAA CATACTGTCA ATAGGAGCCT GTGGATACAT CGGTGCACTG
    GTGAGAAACA GCTGCTGGCT GATCCAAGCG TTCAGCCTGG CCTGCACGGT GAAAGGCTAT
    AAAATGCCTG AAGATGACTC CAGATGCAAG CTGCCCAGTG GGGAGGCAGG GATCATCTGG
    GACAGCATCT GCTTTGCGTT TCTCCTGCTA CAGAGGAGAG TCTTCATGAG TTACTATTTC
    CTGCATGTCG TGGCTGACAT TAAAGCATCC CAGATCCTGG CATCCAGAGG GGCCGAACTT
    TTCCAGGCTA CAATTGTGAA AGCTGTAAAG GCAAGAATTG AAGAAGAGAA AAAATCCATG
    GACCAGCTGA AGAGACAGAT GGATCGCATC AAAGCCCGGC AGCAAAAGTA CAAAAAGGGT
    AAGGAGAGGA TGCTGAGCTT GACCCAGGAG TCAGGGGAAG GCCAGGACAT CCAGAAAGTC
    TCAGAAGAAG ACGATGAAAG AGAAGCAGAC AAACAGAAAG CCAAGGGCAA AAAAAAGCAG
    TGGTGGCGGC CTTGGGTTGA TCATGCTTCC ATGGTCAGGA GTGGAGATTA TTATTTGTTT
    GAAACGGATA GTGAAGAGGA GGAAGAAGAG GAACTCAAAA AGGAGGATGA AGAACCACCA
    CGGAAGTCTG CATTCCAGAG AGCTATTGGG AAGTTTGCCT CAGCCATTTT AGCCTTGCCA
    AAGTCTGTCA TTAAACTTCC CAAAACTATT CTTCAGTATT TAATCAGAGC TGCAAAGTTC
    GTCTACCAAG CCTGGATTAC CGATCCTAAA ACAGCACTCC GACAGAGGAG GAAAGAGAAG
    AAGAAAATGG CCAGAGAAGA GCAGAAGGGA AGACGCAAAG GATCTGGGGA GGGTCCAGTG
    GAATGGGAAG ACAGAGAAGA TGAGCCAATC AAAAAGAAAT CTGATGGACC AGATAATATC
    ATCAAGAGGA TATTTAATAT CCTGAAGTTC ACCTGGGTCC TGTTCCTGGC CACAGTGGAC
    AGTTTCACTA CTTGGCTCAA CTCCATCTCA AGAGAGCATA TCGACATATC CACAGTTCTG
    CGGATTGAAA GATGCATGCT GACCCGAGAG ATAAAGAAGG GCAACGTTCC GACACGGGAA
    AGCATCCACA TGTACTACCA GAACCACATG ATGAACCTTT CCAGAGAATC CGGACTGGAC
    ACAATCGACG AGCACTCTGG CGCTGGTTCC AGGGCCCAGG CAGCACACAG GATGGATAGC
    TTAGATTCCC ATGACAGTAT CTCCAGCGAG CCCACACAGT GCACCATGCT GTATTCACGC
    CAAGGAACTA CGGAAACCAT TGAGGAGGTA GAGGCTGAAG CTGAGGAGGA GGTGGTAGAG
    AGTCTGGAGC CTGAGCTCCA TGATGCCGAA GAGAAGGAAT ATGCAGCTGA CTATGAAGCA
    GGGGTAGAGG AGGTCAGCCT CACTCCAGAT GAGGAGTTGC CTCAGTTCTC AACAGATGAC
    GGTGACGTGG AGGCCCCGCC CTCCTACAGC AAGGCTGTCA GCTTTGAGCA CCTCTCGTTT
    GCATCACAAG ATGACTCCGG GGGCAAGAAC CACATGGTGG TCAGCCCTGA CGACAGCCGC
    ACAGACAAGC TGGAGTCCAG TATCTTACCG CCCCTGACTC ACGAGCTGAC AGCCAGCGAT
    CTGCTCATGA GCAAGATGTT CCGTGATGAT GAGCTTGAAG AATCTGAGAA ATTCTACGTG
    GACCAGCCCC GGTTCTTGCT GCTATTCTAT GCCATGTATA ACACTCTGGT AGCCCGCTCG
    GAAATGGTGT GCTATTTCGT GATCATCCTG AACCACATGA TCTCTGCCTC CATCATTACT
    CTTCTGTTGC CCATCCTGAT CTTCCTCTGG GCCATGCTGT CCGTCCCCAG GCCCAGCCGG
    CGCTTCTGGA TGATGGCCAT CGTCTACACT GAGGTGGCGA TTGTAGTGAA ATATTTCTTC
    CAGTTTGGCT TCTTTCCTTG GAATAAGGAC TTGGAAATTT ACAAGGACAG GCCCTACTTT
    CCACCAAACA TCATAGGGGT GGAAAAGAAA GAGGGCTATG TTCTTTACGA CCTCATTCAG
    CTGCTGGCTC TGTTCTTCCA TCGATCCATT CTGAAGTGTC ATGGCCTATG GGACGAGGAT
    GACATTGTTG ACAGTAACAC AGACAAGGAA GGATCTGATG ATGAGCTGTC CCTCGGTCAG
    GGCAGAAGAG GCTCCTCAGA GTCACTGAAG TCCATCAACT TGGCTGCCTC TGTAGAGTCC
    GTCCATGTGA CCTTCCCAGA GCAGCCAGCA GCCATCCGGA GAAAACGCTC CTGCAGCAGC
    TCGCAGATCT CCCCACGGTC CAGCTTCTCT TCAAATAGGT CCAAAAGAGG CAGCACAAGC
    ACCCGAAACA GCAGCCAAAA AGGAAGCAGT GTTTTGAGCA TTAAACAAAA AAGCAAAAGA
    GAACTTTACA TGGAAAAGCT TCAAGAACAT TTAATCAAAG CAAAGGCCTT TACCATAAAG
    AAAACTCTAC AAATCTATGT ACCCATTCGG CAATTCTTCT ATGACCTCAT CCACCCAGAC
    TACAGTGCTG TGACTGACGT GTATGTGCTG ATGTTCCTGG CCGACACCGT GGACTTCATC
    ATCATCGTCT TTGGCTTCTG GGCTTTTGGG AAACACTCGG CAGCTGCAGA TATCACCTCT
    TCCCTATCAG AAGACCAGGT CCCCGGGCCA TTCCTGGTGA TGGTTCTCAT TCAGTTTGGA
    ACCATGGTGG TGGACCGTGC ACTCTACCTC AGGAAGACTG TGCTTGGCAA GGTCATTTTC
    CAGGTCATCC TCGTGTTTGG AATTCACTTC TGGATGTTCT TCATCCTACC CAGTGTGACT
    GAGAGGAAAT TCAGCCAGAA CCTGGTGGCA CAGCTCTGGT ACTTTGTGAA ATGTGTGTAC
    TTTGGGTTGT CTGCCTACCA AATCCGCTGT GGCTACCCAA CACGTGTGCT CGGAAACTTC
    CTCACGAAGA GCTACAATTA TGTCAACCTC TTCTTGTTTC AAGGGTTTCG TCTCGTCCCA
    TTTCTGACAG AGCTGCGGGC TGTGATGGAC TGGGTGTGGA CAGATACAAC CTTGAGCCTC
    TCCAGCTGGA TTTGTGTGGA GGACATTTAC GCACACATTT TCATCTTAAA GTGCTGGCGA
    GAGTCGGAAA AGAGATACCC TCAACCCAGG GGCCAGAAGA AGAAGAAAGC TGTGAAGTAT
    GGCATGGGCG GAATGATCAT TGTCCTGCTC ATCTGTATCG TCTGGTTTCC TCTGCTCTTC
    ATGTCTTTGA TCAAGTCTGT CGCAGGGGTT ATTAACCAAC CCCTGGATGT GTCCGTCACA
    ATAACCCTGG GAGGCTATCA GCCTATCTTC ACAATGAGTG CCCAGCAAAG CCAGCTGAAA
    GTTATGGATG GTACAAAGTA TAACGAATTC ATAAAGTCTT TCGGCCCCAA CAGTGGCGCT
    ATGCAATTTC TGGAGAATTA TGAAAGGCAA GACATAACAG TAGCGGAACT GGAAGGGAAT
    TCGAATTCTT TGTGGACCAT CAGCCCTCCC AGTAAGCAGA AAATGATACA GGAGCTCACC
    GACCCCAATA GTTGCTTCTC TGTTGTTTTT TCATGGAGTA TCCAGAGAAA CATGACTCTG
    GGTGCAAAGG CTGAAATAGC AACGGACAAG CTTTCTTTTC CTCTCACGGA TGCGACACGA
    AGTAGCATAG CCAAAATGAT TGCTGGCAAT GACACAGAAA GTTCCAACAC ACCAGTGACA
    ATAGAAAAGA TCTACCCATA CTATGTGAAG GCACCCAGTG ACTCAAACTC AAAGCCTATA
    AAACAGCTTC TGTCAGAAAA CAATTTCATG AATATCACCA TCATTTTGTT CAGAGACAAT
    GTCACAAAGT CCAATAGTGA GTGGTGGGTT CTCAACCTGA CTGGAAGTAG GATATTCAAT
    CAGGGATCCC AAGCCTTGGA GCTGGTGGTC TTCAATGACA AAGTCAGCCC CCCAAGTCTG
    GGCTTCTTGG CTGGCTATGG CATCATGGGA TTGTATGCAT CCGTTGTCCT TGTAATTGGG
    AAGTTTGTTC GCGAATTCTT CAGTGGGATT TCTCACTCTA TCATGTTTGA AGAGCTTCCA
    AATGTGGACA GAATTTTGAA GTTATGCACA GATATATTTC TCGTACGGGA GACAGGGGAA
    CTGGAGCTGG AGGAAGACCT CTATGCCAAA TTGATATTCC TGTACAGGTC GCCAGAAACC
    ATGATCAAGT GGACTAGAGA AAAAACAAAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017456609.1
    CDS940..9372
    Translation

    Target ORF information:

    RefSeq Version XM_017601120.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017601120.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTCCCCAT CTGCCTGGCA 
    GTAGCATGTG CATTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCG ACGATGCAAG GACACACAGG GCGGCTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCCTTT CTGCTCCTTC ACATCATTTT CCACATCACA
    CTGGCCAGCC TGGAAGCTCA GCACCGCATC GCACCCGCTT ACAACTGCTC AACTTGGGAA
    AAGACCTTCC GGCAGATCGG CTTCGAAAGC TTGAAGGGAG CTGATGCTGG CAATGGCATC
    AGGGTGTTTG TGCCTGATAT CGGGATGTTC ATTGCGAGCC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACGGAA GAAGTAGCCC AATTTAATTC CGAGTGCGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GACTCAGAAG AAGCGCTGAT TTATGAGGAG
    GACTTAGATG GGGAAGAAGG CATGGAAGGC GAGCTGGAAG AAAGCACAAA GTTAAAAATG
    TTTCGCAGGC TCGCCTCCGT CGCTTCCAAG CTTAAGGAGT TCATCGGCAA TATGATAACC
    ACGGCGGGCA AGGTGGTGGT GACCATCCTG TTGGGCTCCT CTGGCATGAT GCTGCCATCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT CTGGGTCTGT GCACCTGGTG GTCCTGGTGC
    CGAACTTTTG ACCCATTGCT GTTTGGCTGC CTCTGTGTCC TGTTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTTCAGTTCT TCCAAGAAGC AGTCCCACCC
    AATGACTACT ATGCAAGGTT GTTTGGAATC AAATCCGTTA TTCAAACAGA CTGCTCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTACAC GCTGGCCACG CTGATCCGCA TTTGGTTGCA AGAGCCTCTT
    GTGCAGGAGG AGATGTCGAA GGAAGAGGAG GGTGCACTGG ATGGCTGCTC CAATCAGAAC
    ACAGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    CTTTTATCCA TGACCCAAGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACTGTGAAC
    GGCAACCCTG TGGACTACCA CACCATCCAC CCAAGTCTGC CCATAGAGAA TGGCCCCGCC
    AAGACGGACT TGTATACTAC TCCTCAGTAC CGGTGGGAGC CCTCAGAGGA GTCCTCAGAA
    AAGAAAGAGG AGGAAGAGGA CAAGAGAGAA GACTCAGAAG GGGAAGGGAG CCAAGAGGAA
    AAACGGAGTG TGAGAATTCA TGCCATGGTC GCCGTGTTCC AGTTCATCAT GAAGCAGAGT
    TACATCTGTG CCCTCATCGC CATGATGGCA TGGAGCATCA CCTACCACAG CTGGCTGACA
    TTTGTGCTGT TGATCTGGTC GTGCACTCTC TGGATGATTC GCAACAGGAG GAAGTACGCC
    ATGATCAGCT CTCCGTTCAT GGTTGTCTAT GCCAATCTGC TGCTGGTACT GCAGTACATA
    TGGAGCTTTG AACTTCCAGA AATCAAGAAA GTCCCTGGAT TTCTAGAAAA GAAAGAGCCT
    GGAGAGCTTG CCTCAAAGAT CCTCTTCACC ATCACATTTT GGCTGCTCCT GAGGCAGCAT
    CTCACAGAGC AGAAGGCCCT TCAGGAAAAG GAAGCACTTC TGTCTGAAGT CAAAATTGGA
    AGTCAAGAAC TTGACGAAAA GGATGAGGAA CTCCAAGATA TACAAGTGGA AGGAGAGCCC
    ATAGAGAAGG AAGAAGAGGA GGAGGAAATA AAGGAAGACA GACATGAGGT AAAGAAGGAA
    GAGGAAGAGG AAGAGGAAGA TGACGATGAC CAGGACATCA TGAAGGTTCT GGGCAATCTG
    GTGGTGGCCC TCTTCATCAA GTATTGGATC TACGTCTGCG GGGGCATGTT CTTTTTTGTC
    AGCTTTGAGG GGAAAATCGT CATGTACAAA ATCATCTACA TGGTGCTGTT CCTGTTCTGT
    GTGGCCTTGT ACCAGGTGCA CTACGAGTGG TGGAGGAAGA TCCTGAAATA CTTCTGGATG
    TCGGTGGTCA TTTACACCAT GCTGGTGCTC ATCTTCATCT ACACTTACCA GTTTGAGAAC
    TTCCCAGGCC TGTGGCAAAA CATGACCGGG TTAAAGAAAG AGAAGCTTGA GGATCTGGGC
    CTGAAGCAGT TCACTGTGGC CGAACTGTTC ACACGAATAT TCATCCCCAC CTCCTTTCTG
    CTGGTCTGCA TCCTGCACCT CCACTATTTC CATGACCGGT TCCTGGAACT CACAGACCTC
    AAGTCCATCC CCAGCAAAGA AGACAACACC ATCTACAGCC ATGCCAAGGT CAATGGTCGC
    GTGTACCTAA TAATAAATAG CATCAAGAAA AAATTACCAA TCCACCAGAA TGAGTTAGCC
    CACCCTGAAG GAAGCCTCCC AGACCTAGCC ATTATGAACA TGACGGCCAG CCTCGATAAG
    CCTGAGAGGC AGAAGTTGGC AGAGCCTGGG GAGGAGAGGA CAGAAGAATG TGCCAAGAAG
    GCAGAGAAGG GTGAGGCTGG GAAAGACAGT GATGAGTCTG AGGAGGAGGA AGGTGAGGAA
    GAGGAATCCG AGGAGGAAGA ATCCTCAGAT CTCAGGAACA AGTGGCACCT CGTGATTGAC
    CGGCTCACTG TGCTCTTTCT AAAGTTCCTG GAGTATTTCC ACAAGTTGCA GGTGTTTATG
    TGGTGGATCT TGGAGCTGCA CATCATCAAA ATCGTCTCAT CGTACATTAT CTGGGTGACT
    GTCAAAGAGG TGTCTCTGTT CAACTATGTA TTTTTGATTT CTTGGGCATT TGCTCTGCCA
    TATGCCAAAC TCCGCCGTGT GGCTTCCAGT GTCTGCACTG TCTGGACGTG TGTGATCATC
    GTCTGCAAAA TGTTGTACCA GCTGCAAACC ATTAAGCCTG AGAACTTTTC TGTCAACTGC
    TCCTTGCCCA ATGAAAATCA AACCAACATA CCCCTTAATG AGTTGAACAA GTCTCTTCTC
    TACAGTGCCC CTGTTGATCC CACTGAGTGG GTTGGCCTGA GGAAGTCCTC CCCTTTACTC
    GTCTACCTGA GGAACAACCT CCTGATGTTG GCGATTTTGG CCTTCGAAGT CACTGTTTAC
    CGACATCAGG AGTACTATCG AGGCCGAAAC AACTTGACTG CTCCGGTGTC TAAAACTATC
    TTTCATGACA TCACAAGGTT ACATCTAGAT GATGGGCTTA TCAATTGTGC CAAATATTTT
    GTAAACTACT TCTTCTACAA GTTTGGCCTA GAGACCTGTT TCCTAATGTC AGTGAACGTA
    ATTGGACAGC GAATGGACTT CTACGCCATG ATTCATGCCT GTTGGCTGAT CGGTGTCTTG
    TACCGTCGCA GAAGAAAAGC CATTGCTGAG GTCTGGCCCA AGTACTGCTG CTTCCTGGCT
    TGCATCATCA CCTTCCAGTA CTTTGTCTGC ATAGGCATCC CTCCTGCCCC CTGCCGAGAT
    TACCCATGGA GGTTCAAGGG TGCCAACTTC AATGACAACA TCATCAAGTG GCTGTACTTC
    CCAGATTTCA TCGTGCGGCC CAACCCTGTG TTTCTTGTCT ATGACTTCAT GCTGCTCCTG
    TGTGCTTCCT TACAACGACA GATTTTTGAG GACGAAAACA AGGCAGCTGT GCGCATCATG
    GCCGGGGACA ATGTCGAGAT TTGCATGAAC CTTGACGCGG CCTCATTCAG CCAGCATAAC
    CCTGTACCAG ATTTCATTCA CTGCAGATCT TACTTGGACA TGTCCAAAGT GATCATCTTC
    AGCTACCTCT TCTGGTTCGT CCTCACCATC ATCTTCATTA CCGGAACGAC CAGGATCAGC
    ATCTTTTGCA TGGGTTACCT GGTGGCCTGT TTCTATTTCC TGCTCTTTGG AGGTGATCTG
    CTCCTGAAAC CCATCAAGAG CATCCTACGC TACTGGGACT GGCTGATTGC ATACAACGTG
    TTTGTGATCA CCATGAAAAA CATACTGTCA ATAGGAGCCT GTGGATACAT CGGTGCACTG
    GTGAGAAACA GCTGCTGGCT GATCCAAGCG TTCAGCCTGG CCTGCACGGT GAAAGGCTAT
    AAAATGCCTG AAGATGACTC CAGATGCAAG CTGCCCAGTG GGGAGGCAGG GATCATCTGG
    GACAGCATCT GCTTTGCGTT TCTCCTGCTA CAGAGGAGAG TCTTCATGAG TTACTATTTC
    CTGCATGTCG TGGCTGACAT TAAAGCATCC CAGATCCTGG CATCCAGAGG GGCCGAACTT
    TTCCAGGCTA CAATTGTGAA AGCTGTAAAG GCAAGAATTG AAGAAGAGAA AAAATCCATG
    GACCAGCTGA AGAGACAGAT GGATCGCATC AAAGCCCGGC AGCAAAAGTA CAAAAAGGGT
    AAGGAGAGGA TGCTGAGCTT GACCCAGGAG TCAGGGGAAG GCCAGGACAT CCAGAAAGTC
    TCAGAAGAAG ACGATGAAAG AGAAGCAGAC AAACAGAAAG CCAAGGGCAA AAAAAAGCAG
    TGGTGGCGGC CTTGGGTTGA TCATGCTTCC ATGGTCAGGA GTGGAGATTA TTATTTGTTT
    GAAACGGATA GTGAAGAGGA GGAAGAAGAG GAACTCAAAA AGGAGGATGA AGAACCACCA
    CGGAAGTCTG CATTCCAGAG AGCTATTGGG AAGTTTGCCT CAGCCATTTT AGCCTTGCCA
    AAGTCTGTCA TTAAACTTCC CAAAACTATT CTTCAGTATT TAATCAGAGC TGCAAAGTTC
    GTCTACCAAG CCTGGATTAC CGATCCTAAA ACAGCACTCC GACAGAGGAG GAAAGAGAAG
    AAGAAAATGG CCAGAGAAGA GCAGAAGGGA AGACGCAAAG GATCTGGGGA GGGTCCAGTG
    GAATGGGAAG ACAGAGAAGA TGAGCCAATC AAAAAGAAAT CTGATGGACC AGATAATATC
    ATCAAGAGGA TATTTAATAT CCTGAAGTTC ACCTGGGTCC TGTTCCTGGC CACAGTGGAC
    AGTTTCACTA CTTGGCTCAA CTCCATCTCA AGAGAGCATA TCGACATATC CACAGTTCTG
    CGGATTGAAA GATGCATGCT GACCCGAGAG ATAAAGAAGG GCAACGTTCC GACACGGGAA
    AGCATCCACA TGTACTACCA GAACCACATG ATGAACCTTT CCAGAGAATC CGGACTGGAC
    ACAATCGACG AGCACTCTGG CGCTGGTTCC AGGGCCCAGG CAGCACACAG GATGGATAGC
    TTAGATTCCC ATGACAGTAT CTCCAGCGAG CCCACACAGT GCACCATGCT GTATTCACGC
    CAAGGAACTA CGGAAACCAT TGAGGAGGTA GAGGCTGAAG CTGAGGAGGA GGTGGTAGAG
    AGTCTGGAGC CTGAGCTCCA TGATGCCGAA GAGAAGGAAT ATGCAGCTGA CTATGAAGCA
    GGGGTAGAGG AGGTCAGCCT CACTCCAGAT GAGGAGTTGC CTCAGTTCTC AACAGATGAC
    GGTGACGTGG AGGCCCCGCC CTCCTACAGC AAGGCTGTCA GCTTTGAGCA CCTCTCGTTT
    GCATCACAAG ATGACTCCGG GGGCAAGAAC CACATGGTGG TCAGCCCTGA CGACAGCCGC
    ACAGACAAGC TGGAGTCCAG TATCTTACCG CCCCTGACTC ACGAGCTGAC AGCCAGCGAT
    CTGCTCATGA GCAAGATGTT CCGTGATGAT GAGCTTGAAG AATCTGAGAA ATTCTACGTG
    GACCAGCCCC GGTTCTTGCT GCTATTCTAT GCCATGTATA ACACTCTGGT AGCCCGCTCG
    GAAATGGTGT GCTATTTCGT GATCATCCTG AACCACATGA TCTCTGCCTC CATCATTACT
    CTTCTGTTGC CCATCCTGAT CTTCCTCTGG GCCATGCTGT CCGTCCCCAG GCCCAGCCGG
    CGCTTCTGGA TGATGGCCAT CGTCTACACT GAGGTGGCGA TTGTAGTGAA ATATTTCTTC
    CAGTTTGGCT TCTTTCCTTG GAATAAGGAC TTGGAAATTT ACAAGGACAG GCCCTACTTT
    CCACCAAACA TCATAGGGGT GGAAAAGAAA GAGGGCTATG TTCTTTACGA CCTCATTCAG
    CTGCTGGCTC TGTTCTTCCA TCGATCCATT CTGAAGTGTC ATGGCCTATG GGACGAGGAT
    GACATTGTTG ACAGTAACAC AGACAAGGAA GGATCTGATG ATGAGCTGTC CCTCGGTCAG
    GGCAGAAGAG GCTCCTCAGA GTCACTGAAG TCCATCAACT TGGCTGCCTC TGTAGAGTCC
    GTCCATGTGA CCTTCCCAGA GCAGCCAGCA GCCATCCGGA GAAAACGCTC CTGCAGCAGC
    TCGCAGATCT CCCCACGGTC CAGCTTCTCT TCAAATAGGT CCAAAAGAGG CAGCACAAGC
    ACCCGAAACA GCAGCCAAAA AGGAAGCAGT GTTTTGAGCA TTAAACAAAA AAGCAAAAGA
    GAACTTTACA TGGAAAAGCT TCAAGAACAT TTAATCAAAG CAAAGGCCTT TACCATAAAG
    AAAACTCTAC AAATCTATGT ACCCATTCGG CAATTCTTCT ATGACCTCAT CCACCCAGAC
    TACAGTGCTG TGACTGACGT GTATGTGCTG ATGTTCCTGG CCGACACCGT GGACTTCATC
    ATCATCGTCT TTGGCTTCTG GGCTTTTGGG AAACACTCGG CAGCTGCAGA TATCACCTCT
    TCCCTATCAG AAGACCAGGT CCCCGGGCCA TTCCTGGTGA TGGTTCTCAT TCAGTTTGGA
    ACCATGGTGG TGGACCGTGC ACTCTACCTC AGGAAGACTG TGCTTGGCAA GGTCATTTTC
    CAGGTCATCC TCGTGTTTGG AATTCACTTC TGGATGTTCT TCATCCTACC CAGTGTGACT
    GAGAGGAAAT TCAGCCAGAA CCTGGTGGCA CAGCTCTGGT ACTTTGTGAA ATGTGTGTAC
    TTTGGGTTGT CTGCCTACCA AATCCGCTGT GGCTACCCAA CACGTGTGCT CGGAAACTTC
    CTCACGAAGA GCTACAATTA TGTCAACCTC TTCTTGTTTC AAGGGTTTCG TCTCGTCCCA
    TTTCTGACAG AGCTGCGGGC TGTGATGGAC TGGGTGTGGA CAGATACAAC CTTGAGCCTC
    TCCAGCTGGA TTTGTGTGGA GGACATTTAC GCACACATTT TCATCTTAAA GTGCTGGCGA
    GAGTCGGAAA AGAGATACCC TCAACCCAGG GGCCAGAAGA AGAAGAAAGC TGTGAAGTAT
    GGCATGGGCG GAATGATCAT TGTCCTGCTC ATCTGTATCG TCTGGTTTCC TCTGCTCTTC
    ATGTCTTTGA TCAAGTCTGT CGCAGGGGTT ATTAACCAAC CCCTGGATGT GTCCGTCACA
    ATAACCCTGG GAGGCTATCA GCCTATCTTC ACAATGAGTG CCCAGCAAAG CCAGCTGAAA
    GTTATGGATG GTACAAAGTA TAACGAATTC ATAAAGTCTT TCGGCCCCAA CAGTGGCGCT
    ATGCAATTTC TGGAGAATTA TGAAAGGCAA GACATAACAG TAGCGGAACT GGAAGGGAAT
    TCGAATTCTT TGTGGACCAT CAGCCCTCCC AGTAAGCAGA AAATGATACA GGAGCTCACC
    GACCCCAATA GTTGCTTCTC TGTTGTTTTT TCATGGAGTA TCCAGAGAAA CATGACTCTG
    GGTGCAAAGG CTGAAATAGC AACGGACAAG CTTTCTTTTC CTCTCACGGA TGCGACACGA
    AGTAGCATAG CCAAAATGAT TGCTGGCAAT GACACAGAAA GTTCCAACAC ACCAGTGACA
    ATAGAAAAGA TCTACCCATA CTATGTGAAG GCACCCAGTG ACTCAAACTC AAAGCCTATA
    AAACAGCTTC TGTCAGAAAA CAATTTCATG AATATCACCA TCATTTTGTT CAGAGACAAT
    GTCACAAAGT CCAATAGTGA GTGGTGGGTT CTCAACCTGA CTGGAAGTAG GATATTCAAT
    CAGGGATCCC AAGCCTTGGA GCTGGTGGTC TTCAATGACA AAGTCAGCCC CCCAAGTCTG
    GGCTTCTTGG CTGGCTATGG CATCATGGGA TTGTATGCAT CCGTTGTCCT TGTAATTGGG
    AAGTTTGTTC GCGAATTCTT CAGTGGGATT TCTCACTCTA TCATGTTTGA AGAGCTTCCA
    AATGTGGACA GAATTTTGAA GTTATGCACA GATATATTTC TCGTACGGGA GACAGGGGAA
    CTGGAGCTGG AGGAAGACCT CTATGCCAAA TTGATATTCC TGTACAGGTC GCCAGAAACC
    ATGATCAAGT GGACTAGAGA AAAAACAAAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.