piezo2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following piezo2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the piezo2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa11653 XM_002937476.4
Latest version!
Xenopus tropicalis piezo-type mechanosensitive ion channel component 2 (piezo2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa35454 XM_012965359.2
Latest version!
Xenopus tropicalis piezo-type mechanosensitive ion channel component 2 (piezo2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa35455 XM_012965361.2
Latest version!
Xenopus tropicalis piezo-type mechanosensitive ion channel component 2 (piezo2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa35456 XM_012965362.2
Latest version!
Xenopus tropicalis piezo-type mechanosensitive ion channel component 2 (piezo2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa35457 XM_012965363.2
Latest version!
Xenopus tropicalis piezo-type mechanosensitive ion channel component 2 (piezo2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa35459 XM_012965365.2
Latest version!
Xenopus tropicalis piezo-type mechanosensitive ion channel component 2 (piezo2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa60016 XM_012965359.3
Latest version!
Xenopus tropicalis piezo-type mechanosensitive ion channel component 2 (piezo2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa60017 XM_031903661.1
Latest version!
Xenopus tropicalis piezo-type mechanosensitive ion channel component 2 (piezo2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa60018 XM_002937476.5
Latest version!
Xenopus tropicalis piezo-type mechanosensitive ion channel component 2 (piezo2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa60019 XM_031903662.1
Latest version!
Xenopus tropicalis piezo-type mechanosensitive ion channel component 2 (piezo2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa60020 XM_012965361.3
Latest version!
Xenopus tropicalis piezo-type mechanosensitive ion channel component 2 (piezo2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa60021 XM_012965362.3
Latest version!
Xenopus tropicalis piezo-type mechanosensitive ion channel component 2 (piezo2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa60022 XM_012965363.3
Latest version!
Xenopus tropicalis piezo-type mechanosensitive ion channel component 2 (piezo2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa60023 XM_031903663.1
Latest version!
Xenopus tropicalis piezo-type mechanosensitive ion channel component 2 (piezo2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa60024 XM_012965365.3
Latest version!
Xenopus tropicalis piezo-type mechanosensitive ion channel component 2 (piezo2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa11653
    Clone ID Related Accession (Same CDS sequence) XM_002937476.4
    Accession Version XM_002937476.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8394bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product piezo-type mechanosensitive ion channel component 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002937476.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGGCATCGG AAGTGGTGTG TGGGCTCATC TTCAGATTTC TTCTCCCACT ATGCCTGGTT 
    GTAGCATGCA TGTTCCGGTA CAATGGAATA TCTTTTGTCT ACCTCATCTA CCTCTTGCTT
    ATACCTTTAT TTTCGGAGCC TACCAAAATA ACAATGCAAG GTCATACAGG ACGGTTATTA
    AAATCCTTAT GTGTTTCCAG TCTGTCATTT CTGCTGCTGC ACATCATCTT TCAAATTACC
    GTATACAGCC TCGAGGCCAG AAAACACATT GAACCACATT TTAACTGTTC AACATGGGAG
    AAAGCAATAC GACAAATTGG TTTTGAAAGT GTAAAAGGAG CAGATGCTGG TAATGGAATC
    CGCGTGTTTG CACCAGATAT CGGAATGTTT ATTGCAAGCT TGGCTACCCT GTTGGTTTGT
    AGAAAACTAG TTCAGAAAAC AGTTACTGAA GAGGACGCAC AATACAACCC CCAGTTTGTA
    AATGAAGAAA TGGCAGAAGA AGGAAAGGTG GACCTGGATG AAGCGTTAAT GTATGAGGAA
    GACTTTGATG GAGAAGAGGA TGAAATTGAG GCAGCTGAAG AAAGCACAAA ATTAAAAATT
    CTTCGCAGAA TTGCTTCATT TGCATCCGTA CTTAAAGAGT TTATTGGCAA CATTATAACC
    ACCGCTGGGA AAGTAGTTAT TACAATTTTA CTTGGTTTAA CAGGCATGAT GTTGCCGTCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT TTGGGGCTAT GTACATGGTG GTCCTGTTGC
    CGAGTATTTG ATCCGCTGAT ATTCAGCTGT CTATGTGTAT TAATGGCTAT ATTTAGTGCA
    GGGCACTTAA TTGGACTTTA TCTGTACCAG TTGCAGTTTT TTCACGAGAT TATTCCACCA
    AATGATTTCT ATGCCAGGTT GTTTGGTGTT ACTTCATTAG TTCAAACCGA CTGCTGTAAC
    ACTTGGAGAT TTTTTGCAAA CAACAGTCTA CCATGGAACC AGTATACCAA TCCTGTCATG
    CTTTTAGTAC TGTATTACAC GCTGGCAACT CTCATACGCC TTTGGCTACA GGAACCTATT
    AATGTAGAGG AAGAGAAGGA GGAAGACTGT AGCCCAGCAG CACTTACTGC AGAAAGAAGA
    CGTAGTTTGT GGTACTCTAG CCACTACACA ACGGATGAAA GACAACTTCT GTCCATGACT
    CAAGATGACT ACAAGCCATC TGATGTTTTA CTAGTAACTG TAAATGGGAC TCCCGTAGAT
    TACTGTTCAC TAAATTCATC ACTTCCGATA GAAAATGGTC CAACTAAAAT TGACATGTAT
    TCAACACCGC AATATAAATG GGACCATGAA GATGAAACTT CAGAAAAGAA AGAAGAGGAA
    GAGCAAGAAG AAGAACCCAA TGAAGAGGTT GCAGAAGAGA AAGAAAACAC TAAAGTTCAT
    GTAATGGTGG TGGCATTTCA GTTCATTATG AAGCAAAGCT ATATATGTGC ACTAATTTCA
    ATGATGGCCT GGAGCATCAC ATACCACAGC TGGTTGACTT TTGTTTTGTT AATCTGGTCT
    TGTGCTCTCT GGATGGTCCG AAATAGGAGA AAATATGCTA TGCTTAGTTC ACCTTTTATG
    GTTATGTATG GTAATTTACT TTTAACATTA CAATACATAT GGAGCATAGC CTTACAAAGT
    GATGAACTTC CAGAAGTTAT GGGTCTCCTT GAGAGGAAAA AGCCCGAAGA GCTTGTATCG
    AAGATCATTT TCACAGTTAC ATTTTGGCTT TTGCTCAGAC AACACCTTAC AGAGCAAAAG
    GCATTAAAGC AAAAAGAATT AGCATTATCT GAAATAAAAG TTGGAAGTGA AGACAAAGAA
    GGTGAAGTGC AAGAAGAAGA AGAAGAAGTG GAGGATGAAA ATGATATCAT GAAGGTCCTC
    GGAAAGCTAG TGCAGGCTAT GTTTGTCAAG TACTGGATTT ATGTCTGTGG CGGCATGTTT
    TTTTTTGTCA GCTTTGAAGG AAAAATAGTT ATGTACAAAA TTATCTACAT GATGCTCTTC
    CTATTCTGTG TAGCTCTGTA TCAGGTGCAC TATGAATGGT GGAGACGAAT TTTAAAATAT
    TTTTGGATGT CTGTGGTTGT TTACACCATG TTGGTGCTTG TTTTTATTTA CACTTACCAA
    TTTAAATCAT TTCCTGGCCT TTGGATGAAT ATGACAGGGC TGGATAAGGA AAAGTTAGCA
    GATCTTGGTT TAGAGACATT TAACTTGACT GACCTATTTA CTCGTATATT TATCCCAACC
    TCATTTTTGT TAGTGTGCAT TTTGCACCTA CATTACTTCC ATGACCGTTT TCTCCAGCTC
    ACTGACCTAA AGGCTGTAAC CAGCAAGCAG GACAGCACAA TTTACAGATT GGTGCATCCA
    GAAGGAAGTT TGCCTGATAT AACGATGATG AACTTAACAA CAGAATCTGA AATGCTTGAG
    GAAAAGCGGT TGGAAATTTA TGACGAAAAA AAAACTCATG ACCAAGACTC AGATGATGAA
    TGGGATAAAG GGGATAGCAA AGAACCAGGT CATTCTGATG ATGAAGCTGA GGATGAGGAA
    TCAGAAGAAG AAGAAACAAC AACAGAGTCC AGGAACAAGT GGCATCTAGT GATAGATCGC
    TTAACGGTTC TTTTCCTAAA GTTTCTGGAA TATTTTCACA AGTTTCAGGT CTTTGTTTGG
    TGGCTTCTGG AGCTTCACAT GATAAAGATT GTTTCTTCAT ACATCATTTG GCTTACTATA
    AAGGAGGTTT CACTGCTCAA TTATGTATTT TTTATTGCCT GGGCCTTCGC TTTGCCCTAC
    TCACAATTCC GGCCATTGGC ATCAAGTGTT TGCACAGTCT GGACATGTGT CATCATCATA
    TGTAAAATGT TGTACCAGCT AGCCTCCATT GACCCTGCAA CCTTTTCCAG CAACTGTTTA
    ATGCCACAGC TAAACGAAAC AACTGCACGT AACCAAACTG AGCTTAATTC TTCTGTGCTT
    TACAATTCCT CTGTTGATCC TTCTAATTGG GTTGGACTAA AGAAGTCAAC TCCACTCCTT
    GCTTATTTGC GGAACAATTT GCTGATGTTG GCCATGTTAG CTTTTGAAGT AACCATTTAC
    CGGCATCAGG AGTATTACAG GTTGCGAAAC AATCTCACCA CACCTGCAAC AAAAACCATC
    TTTCATGACA TCACAAGACT TCACTTGGAT GATGGACTCA TAAATAGTGC CAAGTATTTT
    GTGAACTACT TTTTCTACAA GTTTGGATTG GAAACATGTT TCTTACTGTC GGTAAATGTA
    ATCGGTCAGA GGATGGACTT TTTTGCCATG GTTCATGCAC TTTGGCTTTT TGGCGTATTA
    TACCGACGCA GAAGGAAAGC GATTGCAGAA ACCTGGCACA GATATTGCTG TTTTCTTGCT
    TGCATTATGG CATTCCAGTA TTTTATATGT ATTGGCATCC CCCCTGCTGC ATGTAAAGAC
    TATCCTTGGA GACAACCTGG AGCCAATTTT AATTCTAACA TAATAAAGTG GTTATACTTT
    CCAGACTTTA TTGTGAGGCC TAGTCCTCTG TTTCTTGTCT ATGATTTTTT GCTTTTGCTT
    TGTGCGTCCC TTCAAAGAGA AGTGTTTGAG GAAGAAAATA AGGCAGCAGT TCGAATCATG
    GCTGGTGACA ATGTAGAAAT TTGCATGAAC CTTGATGCTG CATCTTTTAG CCAACATAAT
    CCTGTACCTG ATTTCATATA CTGCAGATCT TATTTGGACA TGGGTAAAGT TATAATGTTT
    AGTTACCTTT TCTGGTTTGT GCTCACCATC ATCTTCATCA CGGGAACTAC ACGGATCAGC
    ATATTCTGCA TGGGCTACTT GGTAGCTTGC TTCTATTTTC TATTATTTGG TGGAGATTTG
    CTGTTAAAAC CTATAAGAAT CATCCTACGC TATTGGGATT GGCTGATTGC TTACAATGTT
    TTTGTCATTA CAATGAAAAA TGTATTATCA ATAGGAGCAT GTGGCTACAT TGAATATTTA
    ATCAAAGACA GTTGTTGGTT AATTCAGGCA TTCAGTCTTT CTTGTACAGT GAAAGGTTAT
    TTTATCCCCC AAAACACACC TGACTGTCAG TTGCCAAGCG GAGAGGCTGG AATCATTTGG
    GACAGTATAT GTTTTGCTTT CCTTTTATTG CAAAGAAGAG TGTTTATGAG CTATTACTTC
    TTACATGTGG TTGCAGACAT CAAATCATCT CAGATTCTAG CATCAAGAGG TGCTGAACTG
    TTCCAAGCTA CAATTGTAAA AGCTGTAAAA GCAAGAATTG AAGAAGAAAA AAAATCTATG
    GACCAGCTCA AGAGACAGAT GGACCACATT AAAGCCAGGC AAGAAAAATA TAAAAAAGGT
    AAAGAAAAAA TGCTGAGTCT TACACAGGAT GGAGAAGGAC AAGAAGTTCA AAAACTAGAT
    GATGACGATG AAGAAGGAGA CAAAGCAGAC AAAGAGAAAG CCAAGGGCAA AAAAAAGCAG
    TGGTGGCGAC CTTGGGTTGA TCATGCTTCC ATGGTGAAAA GCGGTGATTA TTATTTGTTT
    GAGACGGACA GTGAAGAAGA AGATGAGGAT GACAAAAAAG AAGAAGAGCC TAGAAGAAAA
    TCTGCCTTCC AGAGAGCTAT TGGAAAGTTT GCATCAGCCA TTATAGCCTT GCCAAAGTCT
    GTCATTAAAC TGCCTAAAAC AATTCTTCAA TATTTAATCA GAGCTGCAAA GTTTTTGTAC
    CAAGCATGGA TTACTGATCC AAAAACAGCC CTTAAGCAAA GAAGCATGGA AGCCAAAGAA
    GAAAAGGCAA GGGAAATGAG GCGAAAGGGC ATTGCTGAAG GTGATATTAT TGTAGAATGT
    GGGGATAAAG AAGAAGAAAA AGAGCAAGGA AAAAAGAAAT CAGATGGCCC AGACAATATC
    TTCAAAAGAA TATTTAACAT CCTGAAGTTT ACTTGGGTCC TCTTCCTTGC TACAATCGAT
    AGTTTTACGG CTTGGCTAAA CTCCATTTCA AGAGAACATA TTGATATCTC CAAAGTTCTA
    AGCATAGAAC GTTGCATGCT GACTAGAGAG ATAAAAAAGG GCAATGTGCC TACTCGAGAA
    AGCATCCACA TGTATTACCA AAATCAAATA ATGATGAGCC TTTCTAGAGA GTCTGGACTT
    GACAGAATTG ATGACCAACC TGGTTCATCG TCCAGTCACC ATAGCCATGA CAAGATGGCG
    AGTAAAAGGA TGGACAGTGC AGACTCAGCT GTATCTCATG ATAGCATATC AAGCTGCTGC
    ACTGAAGCAA CCATGCTATT CTCAAGGCAA TCTACACTTG ATGATTTAGA TGGACCAGAC
    TCTGTACCAA AAACAAGTGA GCGTGCTCGA CCTAGGCTTC GTAAAATGTA CAGCATGGAC
    ATGTCTTCTT CATCTGCTGA CAGTATAGCT TCAAGTGAGC CAACTCAGTG CACCATGGTC
    TACTCACGGC AGGGCACAAC AGAAACTATT GAGGAAGTGG AAGATGAAGA GGAGGAAGAA
    AGCAAAAGTC CTTTATCAAA GATAGAAGCC GAGGAAGAAG AATATGACAT GGATTTGGAA
    GGGACAAGCT ATACTCCGGA CACAGAATTT CCCCTGTACG CCACTACAGA GGGGGATCTT
    CCTCCTTCGT ACAGTAAAGC CGGCAGCTTC GAACAACTTA CATTTGTATC TCAGGACTCG
    GCTGACAATT TAATGGTTCT AAGCCCAGAT GAAAGTCGTA CTGATAAGCT ACATGATCAT
    ATTCTTCCAC CTCTTACACA TGAACTGACT GCTAGCGAGC TGCTTCTAAA CAAAATGTTT
    TATGATGAAG AGTTAGAAGA CTCTGAAAAA TTTTATACTA CTCAACCACG CCCGCTGCAG
    CTTATTTATG CCTTGTATAA TACATTGGTG GCCAGATCAG AAATGGTGTG CTATTTTGTG
    ATTATTCTTA ATCACATGAT TTCAGCTTCT ATGATCACAT TGGTACTGCC CATCCTTATA
    TTCCTTTGGG CTATGCTTTC TGTCCCAAGG CCCAGTAAGA GATTTTGGAT GACAGCTATT
    GTATATACAG AGGTGACAAT TGTTATTAAA TATTTTTTCC AATTTGGATT CTTCCCATGG
    AATAAAAATG TGGATTATAC AAAGGACAAA CCTTACTTCC CACCTAACAT AATGGGCATT
    GAGAAGAAAG AAGGTTATGT GCACTATGAC CTGGTGCAAC TGCTTGCTCT GTTTTTCCAT
    AGATCTATTT TAAAGTGTCA TGGGCTTTGG GATGAAGATG ATGTCACTGA AAAAGCCACA
    GATAAAGACG ACACGGATGG AGAGATATCA GAGGCATCAG GAAGAAGGGA TTCCGTTGGC
    TCATTAAAAT CTGTCAATCT AGCTGCGTCT GTGGAGTCAG TCCATGTGCA CTTCCCTGAG
    GAAAAGACCG CTATCAGGAG AAAAAGCACA AGCAGTGGAT CGCAAATTTC CCGAAGGTCA
    AGTTATTCCT CGCACAGGTC TAAAAGAGGC AGCACCAGTA CTCGGAACAG CAGTCAAAAA
    GGAAGCAGCA TTCCCAGTAT TAAACTGAAA TCTAGAAAGG AACTTATACT GGAAAAACTC
    AGAGAACAGC TCATTAAAGC AAAAGCATTT ACGATCAAGA CGATACTACA AATCTATGTC
    CCGATCAGAC AGTTTTTTTA CAATCTTATA CATCCTGAAT ACAGTGCTGT GACTGATGTC
    TATGTGCTGA TGTTCCTGGC AGACACTGTA GATTTCATCA TTATTGTTTT TGGATTTTGG
    GCGTTTGGAA AACACTCCGC TGCAGCAGAT ATTACATCCT CTCTATCAGA AGACCAAGTC
    CCAGAAGCAT TTTTGGTAAT GGTGCTTATC CAGTTTGGAA CCATGGTTGT TGATCGTGCT
    TTGTATCTTC GCAAAACAGT CATGGGAAAA GTTATTTTCC AAGTTATCCT TGTATTTGGA
    ATCCATTTTT GGATGTTCTT CATCTTGCCA GGGGTAACAG AAAGGAAATT TAGTCAGAAC
    ACTGTTGCCC AGCTGTGGTA TTTTGTCAAG TGCATTTATT TTGGATTGTC GGCATATCAG
    ATACGTTGTG GATATCCAAC TCGGGTGCTT GGTAACTTCC TTACTAAAAG CTACAATTAT
    GTCAACCTGT TCCTTTTCCA AGGCTTTCGC CTCGTTCCAT TTCTTACGGA GCTGCGAGCT
    GTTATGGACT GGGTATGGAC TGACACCACT CTCAGTCTTT CCAGCTGGAT CTGTGTAGAG
    GACATTTATG CACATATATT TATACTAAAA TGCTGGCGGG AGTCAGAAAA GAGATATCCC
    CAGCCACGAG GCCAAAAGAA GAAAAAGGTT GTGAAATACG GAATGGGTGG AATGATAATA
    GTTCTGCTGA TTTGTATCGT GTGGTTTCCT TTGCTCTTCA TGTCCTTGAT TAAATCAGTT
    GCTGGGGTCA CTAACAAGCC CCTAGATGTG TCCATTACAA TAACCCTTGG AGGTTATCAG
    CCTATTTTTA CCATGAGTGC ACAACAAAAT CAGCTGCAAG GCTTGAATGA CAATGAATTT
    AAGCATTTAC ATAATATATA TAAAGGCAAT ACACCTGCCA TGCAGTTTCT GGAATCCTAT
    ATGCAGGAAG ACATCACCAT AGCAGGACTG GAAGGCAATT CCAACTCTCT GTGGACCATC
    AGCCCTCCGA GCAGGACAAT GATGATAGAA AGACTTATCC AGGAACCTGA CTTCACAGCG
    GTCATTTCTT GGTCCATTCG AAGAAATGTG AGTCTTGGAG CTAAGGCAGA GGTAACAGGT
    GAAAAATATA CAATATCACT GGCAGGTCAA ACTCGGAGGG AACTTGCGGA TATGATGAAC
    AGTTCATCTC CCAATAAGAC AGTGGAACTG ATAAAGATAT ACCCATTGTG TTTAAAAGCA
    CCAAGTGATG TTACTGCAAA ACCCATCCTT CAGCTTCTTA AGAATCAAGA GTTTGTGAAT
    ATTAGCGTTT TACTAAGAAA AGAAAACACA TCAGAAGGGG TTCGAGAATG GTGGGTAGTG
    AATCAAAGCC ATAAAGAGTT CATTAAGAAG GATGGCCTGG AACTATGGGT TTTCAGTGAC
    AAAGTCAGTC CTCCAAGTCT TGGATTCTTG GCAGGCTATG GAATAATGGG ACTATATGCC
    TCTGTAGTTC TCGTAATAGG AAAGTTTGTG CGTGAGTTTT TCAGCGGAAT TTCGCACTCC
    ATCATGTTTG AGGAGCTTCC CAACGTTGAC CGTATACTGA AGCTGTGCAC AGACATTTTC
    CTTGTGAGAG AGACTGGAGA GTTGGAACTG GAGGAAGACC TGTATGCCAA ATTAATATTT
    TTGTACCGCT CCCCAGAAAC TATGATCAAA TGGACAAGAG AGAAAGATAA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002937522.2
    CDS612..9005
    Translation

    Target ORF information:

    RefSeq Version XM_002937476.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis piezo-type mechanosensitive ion channel component 2 (piezo2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002937476.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGGCATCGG AAGTGGTGTG TGGGCTCATC TTCAGATTTC TTCTCCCACT ATGCCTGGTT 
    GTAGCATGCA TGTTCCGGTA CAATGGAATA TCTTTTGTCT ACCTCATCTA CCTCTTGCTT
    ATACCTTTAT TTTCGGAGCC TACCAAAATA ACAATGCAAG GTCATACAGG ACGGTTATTA
    AAATCCTTAT GTGTTTCCAG TCTGTCATTT CTGCTGCTGC ACATCATCTT TCAAATTACC
    GTATACAGCC TCGAGGCCAG AAAACACATT GAACCACATT TTAACTGTTC AACATGGGAG
    AAAGCAATAC GACAAATTGG TTTTGAAAGT GTAAAAGGAG CAGATGCTGG TAATGGAATC
    CGCGTGTTTG CACCAGATAT CGGAATGTTT ATTGCAAGCT TGGCTACCCT GTTGGTTTGT
    AGAAAACTAG TTCAGAAAAC AGTTACTGAA GAGGACGCAC AATACAACCC CCAGTTTGTA
    AATGAAGAAA TGGCAGAAGA AGGAAAGGTG GACCTGGATG AAGCGTTAAT GTATGAGGAA
    GACTTTGATG GAGAAGAGGA TGAAATTGAG GCAGCTGAAG AAAGCACAAA ATTAAAAATT
    CTTCGCAGAA TTGCTTCATT TGCATCCGTA CTTAAAGAGT TTATTGGCAA CATTATAACC
    ACCGCTGGGA AAGTAGTTAT TACAATTTTA CTTGGTTTAA CAGGCATGAT GTTGCCGTCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT TTGGGGCTAT GTACATGGTG GTCCTGTTGC
    CGAGTATTTG ATCCGCTGAT ATTCAGCTGT CTATGTGTAT TAATGGCTAT ATTTAGTGCA
    GGGCACTTAA TTGGACTTTA TCTGTACCAG TTGCAGTTTT TTCACGAGAT TATTCCACCA
    AATGATTTCT ATGCCAGGTT GTTTGGTGTT ACTTCATTAG TTCAAACCGA CTGCTGTAAC
    ACTTGGAGAT TTTTTGCAAA CAACAGTCTA CCATGGAACC AGTATACCAA TCCTGTCATG
    CTTTTAGTAC TGTATTACAC GCTGGCAACT CTCATACGCC TTTGGCTACA GGAACCTATT
    AATGTAGAGG AAGAGAAGGA GGAAGACTGT AGCCCAGCAG CACTTACTGC AGAAAGAAGA
    CGTAGTTTGT GGTACTCTAG CCACTACACA ACGGATGAAA GACAACTTCT GTCCATGACT
    CAAGATGACT ACAAGCCATC TGATGTTTTA CTAGTAACTG TAAATGGGAC TCCCGTAGAT
    TACTGTTCAC TAAATTCATC ACTTCCGATA GAAAATGGTC CAACTAAAAT TGACATGTAT
    TCAACACCGC AATATAAATG GGACCATGAA GATGAAACTT CAGAAAAGAA AGAAGAGGAA
    GAGCAAGAAG AAGAACCCAA TGAAGAGGTT GCAGAAGAGA AAGAAAACAC TAAAGTTCAT
    GTAATGGTGG TGGCATTTCA GTTCATTATG AAGCAAAGCT ATATATGTGC ACTAATTTCA
    ATGATGGCCT GGAGCATCAC ATACCACAGC TGGTTGACTT TTGTTTTGTT AATCTGGTCT
    TGTGCTCTCT GGATGGTCCG AAATAGGAGA AAATATGCTA TGCTTAGTTC ACCTTTTATG
    GTTATGTATG GTAATTTACT TTTAACATTA CAATACATAT GGAGCATAGC CTTACAAAGT
    GATGAACTTC CAGAAGTTAT GGGTCTCCTT GAGAGGAAAA AGCCCGAAGA GCTTGTATCG
    AAGATCATTT TCACAGTTAC ATTTTGGCTT TTGCTCAGAC AACACCTTAC AGAGCAAAAG
    GCATTAAAGC AAAAAGAATT AGCATTATCT GAAATAAAAG TTGGAAGTGA AGACAAAGAA
    GGTGAAGTGC AAGAAGAAGA AGAAGAAGTG GAGGATGAAA ATGATATCAT GAAGGTCCTC
    GGAAAGCTAG TGCAGGCTAT GTTTGTCAAG TACTGGATTT ATGTCTGTGG CGGCATGTTT
    TTTTTTGTCA GCTTTGAAGG AAAAATAGTT ATGTACAAAA TTATCTACAT GATGCTCTTC
    CTATTCTGTG TAGCTCTGTA TCAGGTGCAC TATGAATGGT GGAGACGAAT TTTAAAATAT
    TTTTGGATGT CTGTGGTTGT TTACACCATG TTGGTGCTTG TTTTTATTTA CACTTACCAA
    TTTAAATCAT TTCCTGGCCT TTGGATGAAT ATGACAGGGC TGGATAAGGA AAAGTTAGCA
    GATCTTGGTT TAGAGACATT TAACTTGACT GACCTATTTA CTCGTATATT TATCCCAACC
    TCATTTTTGT TAGTGTGCAT TTTGCACCTA CATTACTTCC ATGACCGTTT TCTCCAGCTC
    ACTGACCTAA AGGCTGTAAC CAGCAAGCAG GACAGCACAA TTTACAGATT GGTGCATCCA
    GAAGGAAGTT TGCCTGATAT AACGATGATG AACTTAACAA CAGAATCTGA AATGCTTGAG
    GAAAAGCGGT TGGAAATTTA TGACGAAAAA AAAACTCATG ACCAAGACTC AGATGATGAA
    TGGGATAAAG GGGATAGCAA AGAACCAGGT CATTCTGATG ATGAAGCTGA GGATGAGGAA
    TCAGAAGAAG AAGAAACAAC AACAGAGTCC AGGAACAAGT GGCATCTAGT GATAGATCGC
    TTAACGGTTC TTTTCCTAAA GTTTCTGGAA TATTTTCACA AGTTTCAGGT CTTTGTTTGG
    TGGCTTCTGG AGCTTCACAT GATAAAGATT GTTTCTTCAT ACATCATTTG GCTTACTATA
    AAGGAGGTTT CACTGCTCAA TTATGTATTT TTTATTGCCT GGGCCTTCGC TTTGCCCTAC
    TCACAATTCC GGCCATTGGC ATCAAGTGTT TGCACAGTCT GGACATGTGT CATCATCATA
    TGTAAAATGT TGTACCAGCT AGCCTCCATT GACCCTGCAA CCTTTTCCAG CAACTGTTTA
    ATGCCACAGC TAAACGAAAC AACTGCACGT AACCAAACTG AGCTTAATTC TTCTGTGCTT
    TACAATTCCT CTGTTGATCC TTCTAATTGG GTTGGACTAA AGAAGTCAAC TCCACTCCTT
    GCTTATTTGC GGAACAATTT GCTGATGTTG GCCATGTTAG CTTTTGAAGT AACCATTTAC
    CGGCATCAGG AGTATTACAG GTTGCGAAAC AATCTCACCA CACCTGCAAC AAAAACCATC
    TTTCATGACA TCACAAGACT TCACTTGGAT GATGGACTCA TAAATAGTGC CAAGTATTTT
    GTGAACTACT TTTTCTACAA GTTTGGATTG GAAACATGTT TCTTACTGTC GGTAAATGTA
    ATCGGTCAGA GGATGGACTT TTTTGCCATG GTTCATGCAC TTTGGCTTTT TGGCGTATTA
    TACCGACGCA GAAGGAAAGC GATTGCAGAA ACCTGGCACA GATATTGCTG TTTTCTTGCT
    TGCATTATGG CATTCCAGTA TTTTATATGT ATTGGCATCC CCCCTGCTGC ATGTAAAGAC
    TATCCTTGGA GACAACCTGG AGCCAATTTT AATTCTAACA TAATAAAGTG GTTATACTTT
    CCAGACTTTA TTGTGAGGCC TAGTCCTCTG TTTCTTGTCT ATGATTTTTT GCTTTTGCTT
    TGTGCGTCCC TTCAAAGAGA AGTGTTTGAG GAAGAAAATA AGGCAGCAGT TCGAATCATG
    GCTGGTGACA ATGTAGAAAT TTGCATGAAC CTTGATGCTG CATCTTTTAG CCAACATAAT
    CCTGTACCTG ATTTCATATA CTGCAGATCT TATTTGGACA TGGGTAAAGT TATAATGTTT
    AGTTACCTTT TCTGGTTTGT GCTCACCATC ATCTTCATCA CGGGAACTAC ACGGATCAGC
    ATATTCTGCA TGGGCTACTT GGTAGCTTGC TTCTATTTTC TATTATTTGG TGGAGATTTG
    CTGTTAAAAC CTATAAGAAT CATCCTACGC TATTGGGATT GGCTGATTGC TTACAATGTT
    TTTGTCATTA CAATGAAAAA TGTATTATCA ATAGGAGCAT GTGGCTACAT TGAATATTTA
    ATCAAAGACA GTTGTTGGTT AATTCAGGCA TTCAGTCTTT CTTGTACAGT GAAAGGTTAT
    TTTATCCCCC AAAACACACC TGACTGTCAG TTGCCAAGCG GAGAGGCTGG AATCATTTGG
    GACAGTATAT GTTTTGCTTT CCTTTTATTG CAAAGAAGAG TGTTTATGAG CTATTACTTC
    TTACATGTGG TTGCAGACAT CAAATCATCT CAGATTCTAG CATCAAGAGG TGCTGAACTG
    TTCCAAGCTA CAATTGTAAA AGCTGTAAAA GCAAGAATTG AAGAAGAAAA AAAATCTATG
    GACCAGCTCA AGAGACAGAT GGACCACATT AAAGCCAGGC AAGAAAAATA TAAAAAAGGT
    AAAGAAAAAA TGCTGAGTCT TACACAGGAT GGAGAAGGAC AAGAAGTTCA AAAACTAGAT
    GATGACGATG AAGAAGGAGA CAAAGCAGAC AAAGAGAAAG CCAAGGGCAA AAAAAAGCAG
    TGGTGGCGAC CTTGGGTTGA TCATGCTTCC ATGGTGAAAA GCGGTGATTA TTATTTGTTT
    GAGACGGACA GTGAAGAAGA AGATGAGGAT GACAAAAAAG AAGAAGAGCC TAGAAGAAAA
    TCTGCCTTCC AGAGAGCTAT TGGAAAGTTT GCATCAGCCA TTATAGCCTT GCCAAAGTCT
    GTCATTAAAC TGCCTAAAAC AATTCTTCAA TATTTAATCA GAGCTGCAAA GTTTTTGTAC
    CAAGCATGGA TTACTGATCC AAAAACAGCC CTTAAGCAAA GAAGCATGGA AGCCAAAGAA
    GAAAAGGCAA GGGAAATGAG GCGAAAGGGC ATTGCTGAAG GTGATATTAT TGTAGAATGT
    GGGGATAAAG AAGAAGAAAA AGAGCAAGGA AAAAAGAAAT CAGATGGCCC AGACAATATC
    TTCAAAAGAA TATTTAACAT CCTGAAGTTT ACTTGGGTCC TCTTCCTTGC TACAATCGAT
    AGTTTTACGG CTTGGCTAAA CTCCATTTCA AGAGAACATA TTGATATCTC CAAAGTTCTA
    AGCATAGAAC GTTGCATGCT GACTAGAGAG ATAAAAAAGG GCAATGTGCC TACTCGAGAA
    AGCATCCACA TGTATTACCA AAATCAAATA ATGATGAGCC TTTCTAGAGA GTCTGGACTT
    GACAGAATTG ATGACCAACC TGGTTCATCG TCCAGTCACC ATAGCCATGA CAAGATGGCG
    AGTAAAAGGA TGGACAGTGC AGACTCAGCT GTATCTCATG ATAGCATATC AAGCTGCTGC
    ACTGAAGCAA CCATGCTATT CTCAAGGCAA TCTACACTTG ATGATTTAGA TGGACCAGAC
    TCTGTACCAA AAACAAGTGA GCGTGCTCGA CCTAGGCTTC GTAAAATGTA CAGCATGGAC
    ATGTCTTCTT CATCTGCTGA CAGTATAGCT TCAAGTGAGC CAACTCAGTG CACCATGGTC
    TACTCACGGC AGGGCACAAC AGAAACTATT GAGGAAGTGG AAGATGAAGA GGAGGAAGAA
    AGCAAAAGTC CTTTATCAAA GATAGAAGCC GAGGAAGAAG AATATGACAT GGATTTGGAA
    GGGACAAGCT ATACTCCGGA CACAGAATTT CCCCTGTACG CCACTACAGA GGGGGATCTT
    CCTCCTTCGT ACAGTAAAGC CGGCAGCTTC GAACAACTTA CATTTGTATC TCAGGACTCG
    GCTGACAATT TAATGGTTCT AAGCCCAGAT GAAAGTCGTA CTGATAAGCT ACATGATCAT
    ATTCTTCCAC CTCTTACACA TGAACTGACT GCTAGCGAGC TGCTTCTAAA CAAAATGTTT
    TATGATGAAG AGTTAGAAGA CTCTGAAAAA TTTTATACTA CTCAACCACG CCCGCTGCAG
    CTTATTTATG CCTTGTATAA TACATTGGTG GCCAGATCAG AAATGGTGTG CTATTTTGTG
    ATTATTCTTA ATCACATGAT TTCAGCTTCT ATGATCACAT TGGTACTGCC CATCCTTATA
    TTCCTTTGGG CTATGCTTTC TGTCCCAAGG CCCAGTAAGA GATTTTGGAT GACAGCTATT
    GTATATACAG AGGTGACAAT TGTTATTAAA TATTTTTTCC AATTTGGATT CTTCCCATGG
    AATAAAAATG TGGATTATAC AAAGGACAAA CCTTACTTCC CACCTAACAT AATGGGCATT
    GAGAAGAAAG AAGGTTATGT GCACTATGAC CTGGTGCAAC TGCTTGCTCT GTTTTTCCAT
    AGATCTATTT TAAAGTGTCA TGGGCTTTGG GATGAAGATG ATGTCACTGA AAAAGCCACA
    GATAAAGACG ACACGGATGG AGAGATATCA GAGGCATCAG GAAGAAGGGA TTCCGTTGGC
    TCATTAAAAT CTGTCAATCT AGCTGCGTCT GTGGAGTCAG TCCATGTGCA CTTCCCTGAG
    GAAAAGACCG CTATCAGGAG AAAAAGCACA AGCAGTGGAT CGCAAATTTC CCGAAGGTCA
    AGTTATTCCT CGCACAGGTC TAAAAGAGGC AGCACCAGTA CTCGGAACAG CAGTCAAAAA
    GGAAGCAGCA TTCCCAGTAT TAAACTGAAA TCTAGAAAGG AACTTATACT GGAAAAACTC
    AGAGAACAGC TCATTAAAGC AAAAGCATTT ACGATCAAGA CGATACTACA AATCTATGTC
    CCGATCAGAC AGTTTTTTTA CAATCTTATA CATCCTGAAT ACAGTGCTGT GACTGATGTC
    TATGTGCTGA TGTTCCTGGC AGACACTGTA GATTTCATCA TTATTGTTTT TGGATTTTGG
    GCGTTTGGAA AACACTCCGC TGCAGCAGAT ATTACATCCT CTCTATCAGA AGACCAAGTC
    CCAGAAGCAT TTTTGGTAAT GGTGCTTATC CAGTTTGGAA CCATGGTTGT TGATCGTGCT
    TTGTATCTTC GCAAAACAGT CATGGGAAAA GTTATTTTCC AAGTTATCCT TGTATTTGGA
    ATCCATTTTT GGATGTTCTT CATCTTGCCA GGGGTAACAG AAAGGAAATT TAGTCAGAAC
    ACTGTTGCCC AGCTGTGGTA TTTTGTCAAG TGCATTTATT TTGGATTGTC GGCATATCAG
    ATACGTTGTG GATATCCAAC TCGGGTGCTT GGTAACTTCC TTACTAAAAG CTACAATTAT
    GTCAACCTGT TCCTTTTCCA AGGCTTTCGC CTCGTTCCAT TTCTTACGGA GCTGCGAGCT
    GTTATGGACT GGGTATGGAC TGACACCACT CTCAGTCTTT CCAGCTGGAT CTGTGTAGAG
    GACATTTATG CACATATATT TATACTAAAA TGCTGGCGGG AGTCAGAAAA GAGATATCCC
    CAGCCACGAG GCCAAAAGAA GAAAAAGGTT GTGAAATACG GAATGGGTGG AATGATAATA
    GTTCTGCTGA TTTGTATCGT GTGGTTTCCT TTGCTCTTCA TGTCCTTGAT TAAATCAGTT
    GCTGGGGTCA CTAACAAGCC CCTAGATGTG TCCATTACAA TAACCCTTGG AGGTTATCAG
    CCTATTTTTA CCATGAGTGC ACAACAAAAT CAGCTGCAAG GCTTGAATGA CAATGAATTT
    AAGCATTTAC ATAATATATA TAAAGGCAAT ACACCTGCCA TGCAGTTTCT GGAATCCTAT
    ATGCAGGAAG ACATCACCAT AGCAGGACTG GAAGGCAATT CCAACTCTCT GTGGACCATC
    AGCCCTCCGA GCAGGACAAT GATGATAGAA AGACTTATCC AGGAACCTGA CTTCACAGCG
    GTCATTTCTT GGTCCATTCG AAGAAATGTG AGTCTTGGAG CTAAGGCAGA GGTAACAGGT
    GAAAAATATA CAATATCACT GGCAGGTCAA ACTCGGAGGG AACTTGCGGA TATGATGAAC
    AGTTCATCTC CCAATAAGAC AGTGGAACTG ATAAAGATAT ACCCATTGTG TTTAAAAGCA
    CCAAGTGATG TTACTGCAAA ACCCATCCTT CAGCTTCTTA AGAATCAAGA GTTTGTGAAT
    ATTAGCGTTT TACTAAGAAA AGAAAACACA TCAGAAGGGG TTCGAGAATG GTGGGTAGTG
    AATCAAAGCC ATAAAGAGTT CATTAAGAAG GATGGCCTGG AACTATGGGT TTTCAGTGAC
    AAAGTCAGTC CTCCAAGTCT TGGATTCTTG GCAGGCTATG GAATAATGGG ACTATATGCC
    TCTGTAGTTC TCGTAATAGG AAAGTTTGTG CGTGAGTTTT TCAGCGGAAT TTCGCACTCC
    ATCATGTTTG AGGAGCTTCC CAACGTTGAC CGTATACTGA AGCTGTGCAC AGACATTTTC
    CTTGTGAGAG AGACTGGAGA GTTGGAACTG GAGGAAGACC TGTATGCCAA ATTAATATTT
    TTGTACCGCT CCCCAGAAAC TATGATCAAA TGGACAAGAG AGAAAGATAA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa35454
    Clone ID Related Accession (Same CDS sequence) XM_012965359.2
    Accession Version XM_012965359.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8469bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product piezo-type mechanosensitive ion channel component 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012965359.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGGCATCGG AAGTGGTGTG TGGGCTCATC TTCAGATTTC TTCTCCCACT ATGCCTGGTT 
    GTAGCATGCA TGTTCCGGTA CAATGGAATA TCTTTTGTCT ACCTCATCTA CCTCTTGCTT
    ATACCTTTAT TTTCGGAGCC TACCAAAATA ACAATGCAAG GTCATACAGG ACGGTTATTA
    AAATCCTTAT GTGTTTCCAG TCTGTCATTT CTGCTGCTGC ACATCATCTT TCAAATTACC
    GTATACAGCC TCGAGGCCAG AAAACACATT GAACCACATT TTAACTGTTC AACATGGGAG
    AAAGCAATAC GACAAATTGG TTTTGAAAGT GTAAAAGGAG CAGATGCTGG TAATGGAATC
    CGCGTGTTTG CACCAGATAT CGGAATGTTT ATTGCAAGCT TGGCTACCCT GTTGGTTTGT
    AGAAAACTAG TTCAGAAAAC AGTTACTGAA GAGGACGCAC AATACAACCC CCAGTTTGTA
    AATGAAGAAA TGGCAGAAGA AGGAAAGGTG GACCTGGATG AAGCGTTAAT GTATGAGGAA
    GACTTTGATG GAGAAGAGGA TGAAATTGAG GCAGCTGAAG AAAGCACAAA ATTAAAAATT
    CTTCGCAGAA TTGCTTCATT TGCATCCGTA CTTAAAGAGT TTATTGGCAA CATTATAACC
    ACCGCTGGGA AAGTAGTTAT TACAATTTTA CTTGGTTTAA CAGGCATGAT GTTGCCGTCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT TTGGGGCTAT GTACATGGTG GTCCTGTTGC
    CGAGTATTTG ATCCGCTGAT ATTCAGCTGT CTATGTGTAT TAATGGCTAT ATTTAGTGCA
    GGGCACTTAA TTGGACTTTA TCTGTACCAG TTGCAGTTTT TTCACGAGAT TATTCCACCA
    AATGATTTCT ATGCCAGGTT GTTTGGTGTT ACTTCATTAG TTCAAACCGA CTGCTGTAAC
    ACTTGGAGAT TTTTTGCAAA CAACAGTCTA CCATGGAACC AGTATACCAA TCCTGTCATG
    CTTTTAGTAC TGTATTACAC GCTGGCAACT CTCATACGCC TTTGGCTACA GGAACCTATT
    AATGTAGAGG AAGAGAAGGA GGAAGACTGT AGCCCAGCAG CACTTACTGC AGAAAGAAGA
    CGTAGTTTGT GGTACTCTAG CCACTACACA ACGGATGAAA GACAACTTCT GTCCATGACT
    CAAGATGACT ACAAGCCATC TGATGTTTTA CTAGTAACTG TAAATGGGAC TCCCGTAGAT
    TACTGTTCAC TAAATTCATC ACTTCCGATA GAAAATGGTC CAACTAAAAT TGACATGTAT
    TCAACACCGC AATATAAATG GGACCATGAA GATGAAACTT CAGAAAAGAA AGAAGAGGAA
    GAGCAAGAAG AAGAACCCAA TGAAGAGGTT GCAGAAGAGA AAGAAAACAC TAAAGTTCAT
    GTAATGGTGG TGGCATTTCA GTTCATTATG AAGCAAAGCT ATATATGTGC ACTAATTTCA
    ATGATGGCCT GGAGCATCAC ATACCACAGC TGGTTGACTT TTGTTTTGTT AATCTGGTCT
    TGTGCTCTCT GGATGGTCCG AAATAGGAGA AAATATGCTA TGCTTAGTTC ACCTTTTATG
    GTTATGTATG GTAATTTACT TTTAACATTA CAATACATAT GGAGCATAGC CTTACAAAGT
    GATGAACTTC CAGAAGTTAT GGGTCTCCTT GAGAGGAAAA AGCCCGAAGA GCTTGTATCG
    AAGATCATTT TCACAGTTAC ATTTTGGCTT TTGCTCAGAC AACACCTTAC AGAGCAAAAG
    GCATTAAAGC AAAAAGAATT AGCATTATCT GAAATAAAAG TTGGAAGTGA AGACAAAGAA
    GGTGAAGTGC AAGAAGAAGA AGAAGAAGTG GAGGATGAAA ATGATATCAT GAAGGTCCTC
    GGAAAGCTAG TGCAGGCTAT GTTTGTCAAG TACTGGATTT ATGTCTGTGG CGGCATGTTT
    TTTTTTGTCA GCTTTGAAGG AAAAATAGTT ATGTACAAAA TTATCTACAT GATGCTCTTC
    CTATTCTGTG TAGCTCTGTA TCAGGTGCAC TATGAATGGT GGAGACGAAT TTTAAAATAT
    TTTTGGATGT CTGTGGTTGT TTACACCATG TTGGTGCTTG TTTTTATTTA CACTTACCAA
    TTTAAATCAT TTCCTGGCCT TTGGATGAAT ATGACAGGGC TGGATAAGGA AAAGTTAGCA
    GATCTTGGTT TAGAGACATT TAACTTGACT GACCTATTTA CTCGTATATT TATCCCAACC
    TCATTTTTGT TAGTGTGCAT TTTGCACCTA CATTACTTCC ATGACCGTTT TCTCCAGCTC
    ACTGACCTAA AGGCTGTAAC CAGCAAGCAG GACAGCACAA TTTACAGCCA TGCCAAAGTA
    AATGGTCGAG TTTACTTAAT AATAAATAGC ATCAAGCAAA AAATTCCAAT TCAGCAAAAT
    GAATTGGTGC ATCCAGAAGG AAGTTTGCCT GATATAACGA TGATGAACTT AACAACAGAA
    TCTGAAATGC TTGAGGAAAA GCGGTTGGAA ATTTATGACG AAAAAAAAAC TCATGACCAA
    GACTCAGATG ATGAATGGGA TAAAGGGGAT AGCAAAGAAC CAGGTCATTC TGATGATGAA
    GCTGAGGATG AGGAATCAGA AGAAGAAGAA ACAACAACAG AGTCCAGGAA CAAGTGGCAT
    CTAGTGATAG ATCGCTTAAC GGTTCTTTTC CTAAAGTTTC TGGAATATTT TCACAAGTTT
    CAGGTCTTTG TTTGGTGGCT TCTGGAGCTT CACATGATAA AGATTGTTTC TTCATACATC
    ATTTGGCTTA CTATAAAGGA GGTTTCACTG CTCAATTATG TATTTTTTAT TGCCTGGGCC
    TTCGCTTTGC CCTACTCACA ATTCCGGCCA TTGGCATCAA GTGTTTGCAC AGTCTGGACA
    TGTGTCATCA TCATATGTAA AATGTTGTAC CAGCTAGCCT CCATTGACCC TGCAACCTTT
    TCCAGCAACT GTTTAATGCC ACAGCTAAAC GAAACAACTG CACGTAACCA AACTGAGCTT
    AATTCTTCTG TGCTTTACAA TTCCTCTGTT GATCCTTCTA ATTGGGTTGG ACTAAAGAAG
    TCAACTCCAC TCCTTGCTTA TTTGCGGAAC AATTTGCTGA TGTTGGCCAT GTTAGCTTTT
    GAAGTAACCA TTTACCGGCA TCAGGAGTAT TACAGGTTGC GAAACAATCT CACCACACCT
    GCAACAAAAA CCATCTTTCA TGACATCACA AGACTTCACT TGGATGATGG ACTCATAAAT
    AGTGCCAAGT ATTTTGTGAA CTACTTTTTC TACAAGTTTG GATTGGAAAC ATGTTTCTTA
    CTGTCGGTAA ATGTAATCGG TCAGAGGATG GACTTTTTTG CCATGGTTCA TGCACTTTGG
    CTTTTTGGCG TATTATACCG ACGCAGAAGG AAAGCGATTG CAGAAACCTG GCACAGATAT
    TGCTGTTTTC TTGCTTGCAT TATGGCATTC CAGTATTTTA TATGTATTGG CATCCCCCCT
    GCTGCATGTA AAGACTATCC TTGGAGACAA CCTGGAGCCA ATTTTAATTC TAACATAATA
    AAGTGGTTAT ACTTTCCAGA CTTTATTGTG AGGCCTAGTC CTCTGTTTCT TGTCTATGAT
    TTTTTGCTTT TGCTTTGTGC GTCCCTTCAA AGAGAAGTGT TTGAGGAAGA AAATAAGGCA
    GCAGTTCGAA TCATGGCTGG TGACAATGTA GAAATTTGCA TGAACCTTGA TGCTGCATCT
    TTTAGCCAAC ATAATCCTGT ACCTGATTTC ATATACTGCA GATCTTATTT GGACATGGGT
    AAAGTTATAA TGTTTAGTTA CCTTTTCTGG TTTGTGCTCA CCATCATCTT CATCACGGGA
    ACTACACGGA TCAGCATATT CTGCATGGGC TACTTGGTAG CTTGCTTCTA TTTTCTATTA
    TTTGGTGGAG ATTTGCTGTT AAAACCTATA AGAATCATCC TACGCTATTG GGATTGGCTG
    ATTGCTTACA ATGTTTTTGT CATTACAATG AAAAATGTAT TATCAATAGG AGCATGTGGC
    TACATTGAAT ATTTAATCAA AGACAGTTGT TGGTTAATTC AGGCATTCAG TCTTTCTTGT
    ACAGTGAAAG GTTATTTTAT CCCCCAAAAC ACACCTGACT GTCAGTTGCC AAGCGGAGAG
    GCTGGAATCA TTTGGGACAG TATATGTTTT GCTTTCCTTT TATTGCAAAG AAGAGTGTTT
    ATGAGCTATT ACTTCTTACA TGTGGTTGCA GACATCAAAT CATCTCAGAT TCTAGCATCA
    AGAGGTGCTG AACTGTTCCA AGCTACAATT GTAAAAGCTG TAAAAGCAAG AATTGAAGAA
    GAAAAAAAAT CTATGGACCA GCTCAAGAGA CAGATGGACC ACATTAAAGC CAGGCAAGAA
    AAATATAAAA AAGGTAAAGA AAAAATGCTG AGTCTTACAC AGGATGGAGA AGGACAAGAA
    GTTCAAAAAC TAGATGATGA CGATGAAGAA GGAGACAAAG CAGACAAAGA GAAAGCCAAG
    GGCAAAAAAA AGCAGTGGTG GCGACCTTGG GTTGATCATG CTTCCATGGT GAAAAGCGGT
    GATTATTATT TGTTTGAGAC GGACAGTGAA GAAGAAGATG AGGATGACAA AAAAGAAGAA
    GAGCCTAGAA GAAAATCTGC CTTCCAGAGA GCTATTGGAA AGTTTGCATC AGCCATTATA
    GCCTTGCCAA AGTCTGTCAT TAAACTGCCT AAAACAATTC TTCAATATTT AATCAGAGCT
    GCAAAGTTTT TGTACCAAGC ATGGATTACT GATCCAAAAA CAGCCCTTAA GCAAAGAAGC
    ATGGAAGCCA AAGAAGAAAA GGCAAGGGAA ATGAGGCGAA AGGGCATTGC TGAAGGTGAT
    ATTATTGTAG AATGTGGGGA TAAAGAAGAA GAAAAAGAGC AAGGAAAAAA GAAATCAGAT
    GGCCCAGACA ATATCTTCAA AAGAATATTT AACATCCTGA AGTTTACTTG GGTCCTCTTC
    CTTGCTACAA TCGATAGTTT TACGGCTTGG CTAAACTCCA TTTCAAGAGA ACATATTGAT
    ATCTCCAAAG TTCTAAGCAT AGAACGTTGC ATGCTGACTA GAGAGATAAA AAAGGGCAAT
    GTGCCTACTC GAGAAAGCAT CCACATGTAT TACCAAAATC AAATAATGAT GAGCCTTTCT
    AGAGAGTCTG GACTTGACAG AATTGATGAC CAACCTGGTT CATCGTCCAG TCACCATAGC
    CATGACAAGA TGGCGAGTAA AAGGATGGAC AGTGCAGACT CAGCTGTATC TCATGATAGC
    ATATCAAGCT GCTGCACTGA AGCAACCATG CTATTCTCAA GGCAATCTAC ACTTGATGAT
    TTAGATGGAC CAGACTCTGT ACCAAAAACA AGTGAGCGTG CTCGACCTAG GCTTCGTAAA
    ATGTACAGCA TGGACATGTC TTCTTCATCT GCTGACAGTA TAGCTTCAAG TGAGCCAACT
    CAGTGCACCA TGGTCTACTC ACGGCAGGGC ACAACAGAAA CTATTGAGGA AGTGGAAGAT
    GAAGAGGAGG AAGAAAGCAA AAGTCCTTTA TCAAAGATAG AAGCCGAGGA AGAAGAATAT
    GACATGGATT TGGAAGGGAC AAGCTATACT CCGGACACAG AATTTCCCCT GTACGCCACT
    ACAGAGGGGG ATCTTCCTCC TTCGTACAGT AAAGCCGGCA GCTTCGAACA ACTTACATTT
    GTATCTCAGG ACTCGGCTGA CAATTTAATG GTTCTAAGCC CAGATGAAAG TCGTACTGAT
    AAGCTACATG ATCATATTCT TCCACCTCTT ACACATGAAC TGACTGCTAG CGAGCTGCTT
    CTAAACAAAA TGTTTTATGA TGAAGAGTTA GAAGACTCTG AAAAATTTTA TACTACTCAA
    CCACGCCCGC TGCAGCTTAT TTATGCCTTG TATAATACAT TGGTGGCCAG ATCAGAAATG
    GTGTGCTATT TTGTGATTAT TCTTAATCAC ATGATTTCAG CTTCTATGAT CACATTGGTA
    CTGCCCATCC TTATATTCCT TTGGGCTATG CTTTCTGTCC CAAGGCCCAG TAAGAGATTT
    TGGATGACAG CTATTGTATA TACAGAGGTG ACAATTGTTA TTAAATATTT TTTCCAATTT
    GGATTCTTCC CATGGAATAA AAATGTGGAT TATACAAAGG ACAAACCTTA CTTCCCACCT
    AACATAATGG GCATTGAGAA GAAAGAAGGT TATGTGCACT ATGACCTGGT GCAACTGCTT
    GCTCTGTTTT TCCATAGATC TATTTTAAAG TGTCATGGGC TTTGGGATGA AGATGATGTC
    ACTGAAAAAG CCACAGATAA AGACGACACG GATGGAGAGA TATCAGAGGC ATCAGGAAGA
    AGGGATTCCG TTGGCTCATT AAAATCTGTC AATCTAGCTG CGTCTGTGGA GTCAGTCCAT
    GTGCACTTCC CTGAGGAAAA GACCGCTATC AGGAGAAAAA GCACAAGCAG TGGATCGCAA
    ATTTCCCGAA GGTCAAGTTA TTCCTCGCAC AGGTCTAAAA GAGGCAGCAC CAGTACTCGG
    AACAGCAGTC AAAAAGGAAG CAGCATTCCC AGTATTAAAC TGAAATCTAG AAAGGAACTT
    ATACTGGAAA AACTCAGAGA ACAGCTCATT AAAGCAAAAG CATTTACGAT CAAGACGATA
    CTACAAATCT ATGTCCCGAT CAGACAGTTT TTTTACAATC TTATACATCC TGAATACAGT
    GCTGTGACTG ATGTCTATGT GCTGATGTTC CTGGCAGACA CTGTAGATTT CATCATTATT
    GTTTTTGGAT TTTGGGCGTT TGGAAAACAC TCCGCTGCAG CAGATATTAC ATCCTCTCTA
    TCAGAAGACC AAGTCCCAGA AGCATTTTTG GTAATGGTGC TTATCCAGTT TGGAACCATG
    GTTGTTGATC GTGCTTTGTA TCTTCGCAAA ACAGTCATGG GAAAAGTTAT TTTCCAAGTT
    ATCCTTGTAT TTGGAATCCA TTTTTGGATG TTCTTCATCT TGCCAGGGGT AACAGAAAGG
    AAATTTAGTC AGAACACTGT TGCCCAGCTG TGGTATTTTG TCAAGTGCAT TTATTTTGGA
    TTGTCGGCAT ATCAGATACG TTGTGGATAT CCAACTCGGG TGCTTGGTAA CTTCCTTACT
    AAAAGCTACA ATTATGTCAA CCTGTTCCTT TTCCAAGGCT TTCGCCTCGT TCCATTTCTT
    ACGGAGCTGC GAGCTGTTAT GGACTGGGTA TGGACTGACA CCACTCTCAG TCTTTCCAGC
    TGGATCTGTG TAGAGGACAT TTATGCACAT ATATTTATAC TAAAATGCTG GCGGGAGTCA
    GAAAAGAGAT ATCCCCAGCC ACGAGGCCAA AAGAAGAAAA AGGTTGTGAA ATACGGAATG
    GGTGGAATGA TAATAGTTCT GCTGATTTGT ATCGTGTGGT TTCCTTTGCT CTTCATGTCC
    TTGATTAAAT CAGTTGCTGG GGTCACTAAC AAGCCCCTAG ATGTGTCCAT TACAATAACC
    CTTGGAGGTT ATCAGCCTAT TTTTACCATG AGTGCACAAC AAAATCAGCT GCAAGGCTTG
    AATGACAATG AATTTAAGCA TTTACATAAT ATATATAAAG GCAATACACC TGCCATGCAG
    TTTCTGGAAT CCTATATGCA GGAAGACATC ACCATAGCAG GACTGGAAGG CAATTCCAAC
    TCTCTGTGGA CCATCAGCCC TCCGAGCAGG ACAATGATGA TAGAAAGACT TATCCAGGAA
    CCTGACTTCA CAGCGGTCAT TTCTTGGTCC ATTCGAAGAA ATGTGAGTCT TGGAGCTAAG
    GCAGAGGTAA CAGGTGAAAA ATATACAATA TCACTGGCAG GTCAAACTCG GAGGGAACTT
    GCGGATATGA TGAACAGTTC ATCTCCCAAT AAGACAGTGG AACTGATAAA GATATACCCA
    TTGTGTTTAA AAGCACCAAG TGATGTTACT GCAAAACCCA TCCTTCAGCT TCTTAAGAAT
    CAAGAGTTTG TGAATATTAG CGTTTTACTA AGAAAAGAAA ACACATCAGA AGGGGTTCGA
    GAATGGTGGG TAGTGAATCA AAGCCATAAA GAGTTCATTA AGAAGGATGG CCTGGAACTA
    TGGGTTTTCA GTGACAAAGT CAGTCCTCCA AGTCTTGGAT TCTTGGCAGG CTATGGAATA
    ATGGGACTAT ATGCCTCTGT AGTTCTCGTA ATAGGAAAGT TTGTGCGTGA GTTTTTCAGC
    GGAATTTCGC ACTCCATCAT GTTTGAGGAG CTTCCCAACG TTGACCGTAT ACTGAAGCTG
    TGCACAGACA TTTTCCTTGT GAGAGAGACT GGAGAGTTGG AACTGGAGGA AGACCTGTAT
    GCCAAATTAA TATTTTTGTA CCGCTCCCCA GAAACTATGA TCAAATGGAC AAGAGAGAAA
    GATAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012820813.1
    CDS612..9080
    Translation

    Target ORF information:

    RefSeq Version XM_012965359.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis piezo-type mechanosensitive ion channel component 2 (piezo2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012965359.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGGCATCGG AAGTGGTGTG TGGGCTCATC TTCAGATTTC TTCTCCCACT ATGCCTGGTT 
    GTAGCATGCA TGTTCCGGTA CAATGGAATA TCTTTTGTCT ACCTCATCTA CCTCTTGCTT
    ATACCTTTAT TTTCGGAGCC TACCAAAATA ACAATGCAAG GTCATACAGG ACGGTTATTA
    AAATCCTTAT GTGTTTCCAG TCTGTCATTT CTGCTGCTGC ACATCATCTT TCAAATTACC
    GTATACAGCC TCGAGGCCAG AAAACACATT GAACCACATT TTAACTGTTC AACATGGGAG
    AAAGCAATAC GACAAATTGG TTTTGAAAGT GTAAAAGGAG CAGATGCTGG TAATGGAATC
    CGCGTGTTTG CACCAGATAT CGGAATGTTT ATTGCAAGCT TGGCTACCCT GTTGGTTTGT
    AGAAAACTAG TTCAGAAAAC AGTTACTGAA GAGGACGCAC AATACAACCC CCAGTTTGTA
    AATGAAGAAA TGGCAGAAGA AGGAAAGGTG GACCTGGATG AAGCGTTAAT GTATGAGGAA
    GACTTTGATG GAGAAGAGGA TGAAATTGAG GCAGCTGAAG AAAGCACAAA ATTAAAAATT
    CTTCGCAGAA TTGCTTCATT TGCATCCGTA CTTAAAGAGT TTATTGGCAA CATTATAACC
    ACCGCTGGGA AAGTAGTTAT TACAATTTTA CTTGGTTTAA CAGGCATGAT GTTGCCGTCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT TTGGGGCTAT GTACATGGTG GTCCTGTTGC
    CGAGTATTTG ATCCGCTGAT ATTCAGCTGT CTATGTGTAT TAATGGCTAT ATTTAGTGCA
    GGGCACTTAA TTGGACTTTA TCTGTACCAG TTGCAGTTTT TTCACGAGAT TATTCCACCA
    AATGATTTCT ATGCCAGGTT GTTTGGTGTT ACTTCATTAG TTCAAACCGA CTGCTGTAAC
    ACTTGGAGAT TTTTTGCAAA CAACAGTCTA CCATGGAACC AGTATACCAA TCCTGTCATG
    CTTTTAGTAC TGTATTACAC GCTGGCAACT CTCATACGCC TTTGGCTACA GGAACCTATT
    AATGTAGAGG AAGAGAAGGA GGAAGACTGT AGCCCAGCAG CACTTACTGC AGAAAGAAGA
    CGTAGTTTGT GGTACTCTAG CCACTACACA ACGGATGAAA GACAACTTCT GTCCATGACT
    CAAGATGACT ACAAGCCATC TGATGTTTTA CTAGTAACTG TAAATGGGAC TCCCGTAGAT
    TACTGTTCAC TAAATTCATC ACTTCCGATA GAAAATGGTC CAACTAAAAT TGACATGTAT
    TCAACACCGC AATATAAATG GGACCATGAA GATGAAACTT CAGAAAAGAA AGAAGAGGAA
    GAGCAAGAAG AAGAACCCAA TGAAGAGGTT GCAGAAGAGA AAGAAAACAC TAAAGTTCAT
    GTAATGGTGG TGGCATTTCA GTTCATTATG AAGCAAAGCT ATATATGTGC ACTAATTTCA
    ATGATGGCCT GGAGCATCAC ATACCACAGC TGGTTGACTT TTGTTTTGTT AATCTGGTCT
    TGTGCTCTCT GGATGGTCCG AAATAGGAGA AAATATGCTA TGCTTAGTTC ACCTTTTATG
    GTTATGTATG GTAATTTACT TTTAACATTA CAATACATAT GGAGCATAGC CTTACAAAGT
    GATGAACTTC CAGAAGTTAT GGGTCTCCTT GAGAGGAAAA AGCCCGAAGA GCTTGTATCG
    AAGATCATTT TCACAGTTAC ATTTTGGCTT TTGCTCAGAC AACACCTTAC AGAGCAAAAG
    GCATTAAAGC AAAAAGAATT AGCATTATCT GAAATAAAAG TTGGAAGTGA AGACAAAGAA
    GGTGAAGTGC AAGAAGAAGA AGAAGAAGTG GAGGATGAAA ATGATATCAT GAAGGTCCTC
    GGAAAGCTAG TGCAGGCTAT GTTTGTCAAG TACTGGATTT ATGTCTGTGG CGGCATGTTT
    TTTTTTGTCA GCTTTGAAGG AAAAATAGTT ATGTACAAAA TTATCTACAT GATGCTCTTC
    CTATTCTGTG TAGCTCTGTA TCAGGTGCAC TATGAATGGT GGAGACGAAT TTTAAAATAT
    TTTTGGATGT CTGTGGTTGT TTACACCATG TTGGTGCTTG TTTTTATTTA CACTTACCAA
    TTTAAATCAT TTCCTGGCCT TTGGATGAAT ATGACAGGGC TGGATAAGGA AAAGTTAGCA
    GATCTTGGTT TAGAGACATT TAACTTGACT GACCTATTTA CTCGTATATT TATCCCAACC
    TCATTTTTGT TAGTGTGCAT TTTGCACCTA CATTACTTCC ATGACCGTTT TCTCCAGCTC
    ACTGACCTAA AGGCTGTAAC CAGCAAGCAG GACAGCACAA TTTACAGCCA TGCCAAAGTA
    AATGGTCGAG TTTACTTAAT AATAAATAGC ATCAAGCAAA AAATTCCAAT TCAGCAAAAT
    GAATTGGTGC ATCCAGAAGG AAGTTTGCCT GATATAACGA TGATGAACTT AACAACAGAA
    TCTGAAATGC TTGAGGAAAA GCGGTTGGAA ATTTATGACG AAAAAAAAAC TCATGACCAA
    GACTCAGATG ATGAATGGGA TAAAGGGGAT AGCAAAGAAC CAGGTCATTC TGATGATGAA
    GCTGAGGATG AGGAATCAGA AGAAGAAGAA ACAACAACAG AGTCCAGGAA CAAGTGGCAT
    CTAGTGATAG ATCGCTTAAC GGTTCTTTTC CTAAAGTTTC TGGAATATTT TCACAAGTTT
    CAGGTCTTTG TTTGGTGGCT TCTGGAGCTT CACATGATAA AGATTGTTTC TTCATACATC
    ATTTGGCTTA CTATAAAGGA GGTTTCACTG CTCAATTATG TATTTTTTAT TGCCTGGGCC
    TTCGCTTTGC CCTACTCACA ATTCCGGCCA TTGGCATCAA GTGTTTGCAC AGTCTGGACA
    TGTGTCATCA TCATATGTAA AATGTTGTAC CAGCTAGCCT CCATTGACCC TGCAACCTTT
    TCCAGCAACT GTTTAATGCC ACAGCTAAAC GAAACAACTG CACGTAACCA AACTGAGCTT
    AATTCTTCTG TGCTTTACAA TTCCTCTGTT GATCCTTCTA ATTGGGTTGG ACTAAAGAAG
    TCAACTCCAC TCCTTGCTTA TTTGCGGAAC AATTTGCTGA TGTTGGCCAT GTTAGCTTTT
    GAAGTAACCA TTTACCGGCA TCAGGAGTAT TACAGGTTGC GAAACAATCT CACCACACCT
    GCAACAAAAA CCATCTTTCA TGACATCACA AGACTTCACT TGGATGATGG ACTCATAAAT
    AGTGCCAAGT ATTTTGTGAA CTACTTTTTC TACAAGTTTG GATTGGAAAC ATGTTTCTTA
    CTGTCGGTAA ATGTAATCGG TCAGAGGATG GACTTTTTTG CCATGGTTCA TGCACTTTGG
    CTTTTTGGCG TATTATACCG ACGCAGAAGG AAAGCGATTG CAGAAACCTG GCACAGATAT
    TGCTGTTTTC TTGCTTGCAT TATGGCATTC CAGTATTTTA TATGTATTGG CATCCCCCCT
    GCTGCATGTA AAGACTATCC TTGGAGACAA CCTGGAGCCA ATTTTAATTC TAACATAATA
    AAGTGGTTAT ACTTTCCAGA CTTTATTGTG AGGCCTAGTC CTCTGTTTCT TGTCTATGAT
    TTTTTGCTTT TGCTTTGTGC GTCCCTTCAA AGAGAAGTGT TTGAGGAAGA AAATAAGGCA
    GCAGTTCGAA TCATGGCTGG TGACAATGTA GAAATTTGCA TGAACCTTGA TGCTGCATCT
    TTTAGCCAAC ATAATCCTGT ACCTGATTTC ATATACTGCA GATCTTATTT GGACATGGGT
    AAAGTTATAA TGTTTAGTTA CCTTTTCTGG TTTGTGCTCA CCATCATCTT CATCACGGGA
    ACTACACGGA TCAGCATATT CTGCATGGGC TACTTGGTAG CTTGCTTCTA TTTTCTATTA
    TTTGGTGGAG ATTTGCTGTT AAAACCTATA AGAATCATCC TACGCTATTG GGATTGGCTG
    ATTGCTTACA ATGTTTTTGT CATTACAATG AAAAATGTAT TATCAATAGG AGCATGTGGC
    TACATTGAAT ATTTAATCAA AGACAGTTGT TGGTTAATTC AGGCATTCAG TCTTTCTTGT
    ACAGTGAAAG GTTATTTTAT CCCCCAAAAC ACACCTGACT GTCAGTTGCC AAGCGGAGAG
    GCTGGAATCA TTTGGGACAG TATATGTTTT GCTTTCCTTT TATTGCAAAG AAGAGTGTTT
    ATGAGCTATT ACTTCTTACA TGTGGTTGCA GACATCAAAT CATCTCAGAT TCTAGCATCA
    AGAGGTGCTG AACTGTTCCA AGCTACAATT GTAAAAGCTG TAAAAGCAAG AATTGAAGAA
    GAAAAAAAAT CTATGGACCA GCTCAAGAGA CAGATGGACC ACATTAAAGC CAGGCAAGAA
    AAATATAAAA AAGGTAAAGA AAAAATGCTG AGTCTTACAC AGGATGGAGA AGGACAAGAA
    GTTCAAAAAC TAGATGATGA CGATGAAGAA GGAGACAAAG CAGACAAAGA GAAAGCCAAG
    GGCAAAAAAA AGCAGTGGTG GCGACCTTGG GTTGATCATG CTTCCATGGT GAAAAGCGGT
    GATTATTATT TGTTTGAGAC GGACAGTGAA GAAGAAGATG AGGATGACAA AAAAGAAGAA
    GAGCCTAGAA GAAAATCTGC CTTCCAGAGA GCTATTGGAA AGTTTGCATC AGCCATTATA
    GCCTTGCCAA AGTCTGTCAT TAAACTGCCT AAAACAATTC TTCAATATTT AATCAGAGCT
    GCAAAGTTTT TGTACCAAGC ATGGATTACT GATCCAAAAA CAGCCCTTAA GCAAAGAAGC
    ATGGAAGCCA AAGAAGAAAA GGCAAGGGAA ATGAGGCGAA AGGGCATTGC TGAAGGTGAT
    ATTATTGTAG AATGTGGGGA TAAAGAAGAA GAAAAAGAGC AAGGAAAAAA GAAATCAGAT
    GGCCCAGACA ATATCTTCAA AAGAATATTT AACATCCTGA AGTTTACTTG GGTCCTCTTC
    CTTGCTACAA TCGATAGTTT TACGGCTTGG CTAAACTCCA TTTCAAGAGA ACATATTGAT
    ATCTCCAAAG TTCTAAGCAT AGAACGTTGC ATGCTGACTA GAGAGATAAA AAAGGGCAAT
    GTGCCTACTC GAGAAAGCAT CCACATGTAT TACCAAAATC AAATAATGAT GAGCCTTTCT
    AGAGAGTCTG GACTTGACAG AATTGATGAC CAACCTGGTT CATCGTCCAG TCACCATAGC
    CATGACAAGA TGGCGAGTAA AAGGATGGAC AGTGCAGACT CAGCTGTATC TCATGATAGC
    ATATCAAGCT GCTGCACTGA AGCAACCATG CTATTCTCAA GGCAATCTAC ACTTGATGAT
    TTAGATGGAC CAGACTCTGT ACCAAAAACA AGTGAGCGTG CTCGACCTAG GCTTCGTAAA
    ATGTACAGCA TGGACATGTC TTCTTCATCT GCTGACAGTA TAGCTTCAAG TGAGCCAACT
    CAGTGCACCA TGGTCTACTC ACGGCAGGGC ACAACAGAAA CTATTGAGGA AGTGGAAGAT
    GAAGAGGAGG AAGAAAGCAA AAGTCCTTTA TCAAAGATAG AAGCCGAGGA AGAAGAATAT
    GACATGGATT TGGAAGGGAC AAGCTATACT CCGGACACAG AATTTCCCCT GTACGCCACT
    ACAGAGGGGG ATCTTCCTCC TTCGTACAGT AAAGCCGGCA GCTTCGAACA ACTTACATTT
    GTATCTCAGG ACTCGGCTGA CAATTTAATG GTTCTAAGCC CAGATGAAAG TCGTACTGAT
    AAGCTACATG ATCATATTCT TCCACCTCTT ACACATGAAC TGACTGCTAG CGAGCTGCTT
    CTAAACAAAA TGTTTTATGA TGAAGAGTTA GAAGACTCTG AAAAATTTTA TACTACTCAA
    CCACGCCCGC TGCAGCTTAT TTATGCCTTG TATAATACAT TGGTGGCCAG ATCAGAAATG
    GTGTGCTATT TTGTGATTAT TCTTAATCAC ATGATTTCAG CTTCTATGAT CACATTGGTA
    CTGCCCATCC TTATATTCCT TTGGGCTATG CTTTCTGTCC CAAGGCCCAG TAAGAGATTT
    TGGATGACAG CTATTGTATA TACAGAGGTG ACAATTGTTA TTAAATATTT TTTCCAATTT
    GGATTCTTCC CATGGAATAA AAATGTGGAT TATACAAAGG ACAAACCTTA CTTCCCACCT
    AACATAATGG GCATTGAGAA GAAAGAAGGT TATGTGCACT ATGACCTGGT GCAACTGCTT
    GCTCTGTTTT TCCATAGATC TATTTTAAAG TGTCATGGGC TTTGGGATGA AGATGATGTC
    ACTGAAAAAG CCACAGATAA AGACGACACG GATGGAGAGA TATCAGAGGC ATCAGGAAGA
    AGGGATTCCG TTGGCTCATT AAAATCTGTC AATCTAGCTG CGTCTGTGGA GTCAGTCCAT
    GTGCACTTCC CTGAGGAAAA GACCGCTATC AGGAGAAAAA GCACAAGCAG TGGATCGCAA
    ATTTCCCGAA GGTCAAGTTA TTCCTCGCAC AGGTCTAAAA GAGGCAGCAC CAGTACTCGG
    AACAGCAGTC AAAAAGGAAG CAGCATTCCC AGTATTAAAC TGAAATCTAG AAAGGAACTT
    ATACTGGAAA AACTCAGAGA ACAGCTCATT AAAGCAAAAG CATTTACGAT CAAGACGATA
    CTACAAATCT ATGTCCCGAT CAGACAGTTT TTTTACAATC TTATACATCC TGAATACAGT
    GCTGTGACTG ATGTCTATGT GCTGATGTTC CTGGCAGACA CTGTAGATTT CATCATTATT
    GTTTTTGGAT TTTGGGCGTT TGGAAAACAC TCCGCTGCAG CAGATATTAC ATCCTCTCTA
    TCAGAAGACC AAGTCCCAGA AGCATTTTTG GTAATGGTGC TTATCCAGTT TGGAACCATG
    GTTGTTGATC GTGCTTTGTA TCTTCGCAAA ACAGTCATGG GAAAAGTTAT TTTCCAAGTT
    ATCCTTGTAT TTGGAATCCA TTTTTGGATG TTCTTCATCT TGCCAGGGGT AACAGAAAGG
    AAATTTAGTC AGAACACTGT TGCCCAGCTG TGGTATTTTG TCAAGTGCAT TTATTTTGGA
    TTGTCGGCAT ATCAGATACG TTGTGGATAT CCAACTCGGG TGCTTGGTAA CTTCCTTACT
    AAAAGCTACA ATTATGTCAA CCTGTTCCTT TTCCAAGGCT TTCGCCTCGT TCCATTTCTT
    ACGGAGCTGC GAGCTGTTAT GGACTGGGTA TGGACTGACA CCACTCTCAG TCTTTCCAGC
    TGGATCTGTG TAGAGGACAT TTATGCACAT ATATTTATAC TAAAATGCTG GCGGGAGTCA
    GAAAAGAGAT ATCCCCAGCC ACGAGGCCAA AAGAAGAAAA AGGTTGTGAA ATACGGAATG
    GGTGGAATGA TAATAGTTCT GCTGATTTGT ATCGTGTGGT TTCCTTTGCT CTTCATGTCC
    TTGATTAAAT CAGTTGCTGG GGTCACTAAC AAGCCCCTAG ATGTGTCCAT TACAATAACC
    CTTGGAGGTT ATCAGCCTAT TTTTACCATG AGTGCACAAC AAAATCAGCT GCAAGGCTTG
    AATGACAATG AATTTAAGCA TTTACATAAT ATATATAAAG GCAATACACC TGCCATGCAG
    TTTCTGGAAT CCTATATGCA GGAAGACATC ACCATAGCAG GACTGGAAGG CAATTCCAAC
    TCTCTGTGGA CCATCAGCCC TCCGAGCAGG ACAATGATGA TAGAAAGACT TATCCAGGAA
    CCTGACTTCA CAGCGGTCAT TTCTTGGTCC ATTCGAAGAA ATGTGAGTCT TGGAGCTAAG
    GCAGAGGTAA CAGGTGAAAA ATATACAATA TCACTGGCAG GTCAAACTCG GAGGGAACTT
    GCGGATATGA TGAACAGTTC ATCTCCCAAT AAGACAGTGG AACTGATAAA GATATACCCA
    TTGTGTTTAA AAGCACCAAG TGATGTTACT GCAAAACCCA TCCTTCAGCT TCTTAAGAAT
    CAAGAGTTTG TGAATATTAG CGTTTTACTA AGAAAAGAAA ACACATCAGA AGGGGTTCGA
    GAATGGTGGG TAGTGAATCA AAGCCATAAA GAGTTCATTA AGAAGGATGG CCTGGAACTA
    TGGGTTTTCA GTGACAAAGT CAGTCCTCCA AGTCTTGGAT TCTTGGCAGG CTATGGAATA
    ATGGGACTAT ATGCCTCTGT AGTTCTCGTA ATAGGAAAGT TTGTGCGTGA GTTTTTCAGC
    GGAATTTCGC ACTCCATCAT GTTTGAGGAG CTTCCCAACG TTGACCGTAT ACTGAAGCTG
    TGCACAGACA TTTTCCTTGT GAGAGAGACT GGAGAGTTGG AACTGGAGGA AGACCTGTAT
    GCCAAATTAA TATTTTTGTA CCGCTCCCCA GAAACTATGA TCAAATGGAC AAGAGAGAAA
    GATAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa35455
    Clone ID Related Accession (Same CDS sequence) XM_012965361.2
    Accession Version XM_012965361.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8370bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product piezo-type mechanosensitive ion channel component 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012965361.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGGCATCGG AAGTGGTGTG TGGGCTCATC TTCAGATTTC TTCTCCCACT ATGCCTGGTT 
    GTAGCATGCA TGTTCCGGTA CAATGGAATA TCTTTTGTCT ACCTCATCTA CCTCTTGCTT
    ATACCTTTAT TTTCGGAGCC TACCAAAATA ACAATGCAAG GTCATACAGG ACGGTTATTA
    AAATCCTTAT GTGTTTCCAG TCTGTCATTT CTGCTGCTGC ACATCATCTT TCAAATTACC
    GTATACAGCC TCGAGGCCAG AAAACACATT GAACCACATT TTAACTGTTC AACATGGGAG
    AAAGCAATAC GACAAATTGG TTTTGAAAGT GTAAAAGGAG CAGATGCTGG TAATGGAATC
    CGCGTGTTTG CACCAGATAT CGGAATGTTT ATTGCAAGCT TGGCTACCCT GTTGGTTTGT
    AGAAAACTAG TTCAGAAAAC AGTTACTGAA GAGGACGCAC AATACAACCC CCAGTTTGTA
    AATGAAGAAA TGGCAGAAGA AGGAAAGGTG GACCTGGATG AAGCGTTAAT GTATGAGGAA
    GACTTTGATG GAGAAGAGGA TGAAATTGAG GCAGCTGAAG AAAGCACAAA ATTAAAAATT
    CTTCGCAGAA TTGCTTCATT TGCATCCGTA CTTAAAGAGT TTATTGGCAA CATTATAACC
    ACCGCTGGGA AAGTAGTTAT TACAATTTTA CTTGGTTTAA CAGGCATGAT GTTGCCGTCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT TTGGGGCTAT GTACATGGTG GTCCTGTTGC
    CGAGTATTTG ATCCGCTGAT ATTCAGCTGT CTATGTGTAT TAATGGCTAT ATTTAGTGCA
    GGGCACTTAA TTGGACTTTA TCTGTACCAG TTGCAGTTTT TTCACGAGAT TATTCCACCA
    AATGATTTCT ATGCCAGGTT GTTTGGTGTT ACTTCATTAG TTCAAACCGA CTGCTGTAAC
    ACTTGGAGAT TTTTTGCAAA CAACAGTCTA CCATGGAACC AGTATACCAA TCCTGTCATG
    CTTTTAGTAC TGTATTACAC GCTGGCAACT CTCATACGCC TTTGGCTACA GGAACCTATT
    AATGTAGAGG AAGAGAAGGA GGAAGACTGT AGCCCAGCAG CACTTACTGC AGAAAGAAGA
    CGTAGTTTGT GGTACTCTAG CCACTACACA ACGGATGAAA GACAACTTCT GTCCATGACT
    CAAGATGACT ACAAGCCATC TGATGTTTTA CTAGTAACTG TAAATGGGAC TCCCGTAGAT
    TACTGTTCAC TAAATTCATC ACTTCCGATA GAAAATGGTC CAACTAAAAT TGACATGTAT
    TCAACACCGC AATATAAATG GGACCATGAA GATGAAACTT CAGAAAAGAA AGAAGAGGAA
    GAGCAAGAAG AAGAACCCAA TGAAGAGGTT GCAGAAGAGA AAGAAAACAC TAAAGTTCAT
    GTAATGGTGG TGGCATTTCA GTTCATTATG AAGCAAAGCT ATATATGTGC ACTAATTTCA
    ATGATGGCCT GGAGCATCAC ATACCACAGC TGGTTGACTT TTGTTTTGTT AATCTGGTCT
    TGTGCTCTCT GGATGGTCCG AAATAGGAGA AAATATGCTA TGCTTAGTTC ACCTTTTATG
    GTTATGTATG GTAATTTACT TTTAACATTA CAATACATAT GGAGCATAGC CTTACAAAGT
    GATGAACTTC CAGAAGTTAT GGGTCTCCTT GAGAGGAAAA AGCCCGAAGA GCTTGTATCG
    AAGATCATTT TCACAGTTAC ATTTTGGCTT TTGCTCAGAC AACACCTTAC AGAGCAAAAG
    GCATTAAAGC AAAAAGAATT AGCATTATCT GAAATAAAAG TTGGAAGTGA AGACAAAGAA
    GGTGAAGTGC AAGAAGAAGA AGAAGAAGTG GAGGATGAAA ATGATATCAT GAAGGTCCTC
    GGAAAGCTAG TGCAGGCTAT GTTTGTCAAG TACTGGATTT ATGTCTGTGG CGGCATGTTT
    TTTTTTGTCA GCTTTGAAGG AAAAATAGTT ATGTACAAAA TTATCTACAT GATGCTCTTC
    CTATTCTGTG TAGCTCTGTA TCAGGTGCAC TATGAATGGT GGAGACGAAT TTTAAAATAT
    TTTTGGATGT CTGTGGTTGT TTACACCATG TTGGTGCTTG TTTTTATTTA CACTTACCAA
    TTTAAATCAT TTCCTGGCCT TTGGATGAAT ATGACAGGGC TGGATAAGGA AAAGTTAGCA
    GATCTTGGTT TAGAGACATT TAACTTGACT GACCTATTTA CTCGTATATT TATCCCAACC
    TCATTTTTGT TAGTGTGCAT TTTGCACCTA CATTACTTCC ATGACCGTTT TCTCCAGCTC
    ACTGACCTAA AGGCTGTAAC CAGCAAGCAG GACAGCACAA TTTACAGCCA TGCCAAAGTA
    AATGGTCGAG TTTACTTAAT AATAAATAGC ATCAAGCAAA AAATTCCAAT TCAGCAAAAT
    GAATTGGTGC ATCCAGAAGG AAGTTTGCCT GATATAACGA TGATGAACTT AACAACAGAA
    TCTGAAATGC TTGAGGAAAA GCGGTTGGAA ATTTATGACG AAAAAAAAAC TCATGACCAA
    GACTCAGATG ATGAATGGGA TAAAGGGGAT AGCAAAGAAC CAGGTCATTC TGATGATGAA
    GCTGAGGATG AGGAATCAGA AGAAGAAGAA ACAACAACAG AGTCCAGGAA CAAGTGGCAT
    CTAGTGATAG ATCGCTTAAC GGTTCTTTTC CTAAAGTTTC TGGAATATTT TCACAAGTTT
    CAGGTCTTTG TTTGGTGGCT TCTGGAGCTT CACATGATAA AGATTGTTTC TTCATACATC
    ATTTGGCTTA CTATAAAGGA GGTTTCACTG CTCAATTATG TATTTTTTAT TGCCTGGGCC
    TTCGCTTTGC CCTACTCACA ATTCCGGCCA TTGGCATCAA GTGTTTGCAC AGTCTGGACA
    TGTGTCATCA TCATATGTAA AATGTTGTAC CAGCTAGCCT CCATTGACCC TGCAACCTTT
    TCCAGCAACT GTTTAATGCC ACAGCTAAAC GAAACAACTG CACGTAACCA AACTGAGCTT
    AATTCTTCTG TGCTTTACAA TTCCTCTGTT GATCCTTCTA ATTGGGTTGG ACTAAAGAAG
    TCAACTCCAC TCCTTGCTTA TTTGCGGAAC AATTTGCTGA TGTTGGCCAT GTTAGCTTTT
    GAAGTAACCA TTTACCGGCA TCAGGAGTAT TACAGGTTGC GAAACAATCT CACCACACCT
    GCAACAAAAA CCATCTTTCA TGACATCACA AGACTTCACT TGGATGATGG ACTCATAAAT
    AGTGCCAAGT ATTTTGTGAA CTACTTTTTC TACAAGTTTG GATTGGAAAC ATGTTTCTTA
    CTGTCGGTAA ATGTAATCGG TCAGAGGATG GACTTTTTTG CCATGGTTCA TGCACTTTGG
    CTTTTTGGCG TATTATACCG ACGCAGAAGG AAAGCGATTG CAGAAACCTG GCACAGATAT
    TGCTGTTTTC TTGCTTGCAT TATGGCATTC CAGTATTTTA TATGTATTGG CATCCCCCCT
    GCTGCATGTA AAGACTATCC TTGGAGACAA CCTGGAGCCA ATTTTAATTC TAACATAATA
    AAGTGGTTAT ACTTTCCAGA CTTTATTGTG AGGCCTAGTC CTCTGTTTCT TGTCTATGAT
    TTTTTGCTTT TGCTTTGTGC GTCCCTTCAA AGAGAAGTGT TTGAGGAAGA AAATAAGGCA
    GCAGTTCGAA TCATGGCTGG TGACAATGTA GAAATTTGCA TGAACCTTGA TGCTGCATCT
    TTTAGCCAAC ATAATCCTGT ACCTGATTTC ATATACTGCA GATCTTATTT GGACATGGGT
    AAAGTTATAA TGTTTAGTTA CCTTTTCTGG TTTGTGCTCA CCATCATCTT CATCACGGGA
    ACTACACGGA TCAGCATATT CTGCATGGGC TACTTGGTAG CTTGCTTCTA TTTTCTATTA
    TTTGGTGGAG ATTTGCTGTT AAAACCTATA AGAATCATCC TACGCTATTG GGATTGGCTG
    ATTGCTTACA ATGTTTTTGT CATTACAATG AAAAATGTAT TATCAATAGG AGCATGTGGC
    TACATTGAAT ATTTAATCAA AGACAGTTGT TGGTTAATTC AGGCATTCAG TCTTTCTTGT
    ACAGTGAAAG GTTATTTTAT CCCCCAAAAC ACACCTGACT GTCAGTTGCC AAGCGGAGAG
    GCTGGAATCA TTTGGGACAG TATATGTTTT GCTTTCCTTT TATTGCAAAG AAGAGTGTTT
    ATGAGCTATT ACTTCTTACA TGTGGTTGCA GACATCAAAT CATCTCAGAT TCTAGCATCA
    AGAGGTGCTG AACTGTTCCA AGCTACAATT GTAAAAGCTG TAAAAGCAAG AATTGAAGAA
    GAAAAAAAAT CTATGGACCA GCTCAAGAGA CAGATGGACC ACATTAAAGC CAGGCAAGAA
    AAATATAAAA AAGGTAAAGA AAAAATGCTG AGTCTTACAC AGGATGGAGA AGGACAAGAA
    GTTCAAAAAC TAGATGATGA CGATGAAGAA GGAGACAAAG CAGACAAAGA GAAAGCCAAG
    GGCAAAAAAA AGCAGTGGTG GCGACCTTGG GTTGATCATG CTTCCATGGT GAAAAGCGGT
    GATTATTATT TGTTTGAGAC GGACAGTGAA GAAGAAGATG AGGATGACAA AAAAGAAGAA
    GAGCCTAGAA GAAAATCTGC CTTCCAGTTT TTGTACCAAG CATGGATTAC TGATCCAAAA
    ACAGCCCTTA AGCAAAGAAG CATGGAAGCC AAAGAAGAAA AGGCAAGGGA AATGAGGCGA
    AAGGGCATTG CTGAAGGTGA TATTATTGTA GAATGTGGGG ATAAAGAAGA AGAAAAAGAG
    CAAGGAAAAA AGAAATCAGA TGGCCCAGAC AATATCTTCA AAAGAATATT TAACATCCTG
    AAGTTTACTT GGGTCCTCTT CCTTGCTACA ATCGATAGTT TTACGGCTTG GCTAAACTCC
    ATTTCAAGAG AACATATTGA TATCTCCAAA GTTCTAAGCA TAGAACGTTG CATGCTGACT
    AGAGAGATAA AAAAGGGCAA TGTGCCTACT CGAGAAAGCA TCCACATGTA TTACCAAAAT
    CAAATAATGA TGAGCCTTTC TAGAGAGTCT GGACTTGACA GAATTGATGA CCAACCTGGT
    TCATCGTCCA GTCACCATAG CCATGACAAG ATGGCGAGTA AAAGGATGGA CAGTGCAGAC
    TCAGCTGTAT CTCATGATAG CATATCAAGC TGCTGCACTG AAGCAACCAT GCTATTCTCA
    AGGCAATCTA CACTTGATGA TTTAGATGGA CCAGACTCTG TACCAAAAAC AAGTGAGCGT
    GCTCGACCTA GGCTTCGTAA AATGTACAGC ATGGACATGT CTTCTTCATC TGCTGACAGT
    ATAGCTTCAA GTGAGCCAAC TCAGTGCACC ATGGTCTACT CACGGCAGGG CACAACAGAA
    ACTATTGAGG AAGTGGAAGA TGAAGAGGAG GAAGAAAGCA AAAGTCCTTT ATCAAAGATA
    GAAGCCGAGG AAGAAGAATA TGACATGGAT TTGGAAGGGA CAAGCTATAC TCCGGACACA
    GAATTTCCCC TGTACGCCAC TACAGAGGGG GATCTTCCTC CTTCGTACAG TAAAGCCGGC
    AGCTTCGAAC AACTTACATT TGTATCTCAG GACTCGGCTG ACAATTTAAT GGTTCTAAGC
    CCAGATGAAA GTCGTACTGA TAAGCTACAT GATCATATTC TTCCACCTCT TACACATGAA
    CTGACTGCTA GCGAGCTGCT TCTAAACAAA ATGTTTTATG ATGAAGAGTT AGAAGACTCT
    GAAAAATTTT ATACTACTCA ACCACGCCCG CTGCAGCTTA TTTATGCCTT GTATAATACA
    TTGGTGGCCA GATCAGAAAT GGTGTGCTAT TTTGTGATTA TTCTTAATCA CATGATTTCA
    GCTTCTATGA TCACATTGGT ACTGCCCATC CTTATATTCC TTTGGGCTAT GCTTTCTGTC
    CCAAGGCCCA GTAAGAGATT TTGGATGACA GCTATTGTAT ATACAGAGGT GACAATTGTT
    ATTAAATATT TTTTCCAATT TGGATTCTTC CCATGGAATA AAAATGTGGA TTATACAAAG
    GACAAACCTT ACTTCCCACC TAACATAATG GGCATTGAGA AGAAAGAAGG TTATGTGCAC
    TATGACCTGG TGCAACTGCT TGCTCTGTTT TTCCATAGAT CTATTTTAAA GTGTCATGGG
    CTTTGGGATG AAGATGATGT CACTGAAAAA GCCACAGATA AAGACGACAC GGATGGAGAG
    ATATCAGAGG CATCAGGAAG AAGGGATTCC GTTGGCTCAT TAAAATCTGT CAATCTAGCT
    GCGTCTGTGG AGTCAGTCCA TGTGCACTTC CCTGAGGAAA AGACCGCTAT CAGGAGAAAA
    AGCACAAGCA GTGGATCGCA AATTTCCCGA AGGTCAAGTT ATTCCTCGCA CAGGTCTAAA
    AGAGGCAGCA CCAGTACTCG GAACAGCAGT CAAAAAGGAA GCAGCATTCC CAGTATTAAA
    CTGAAATCTA GAAAGGAACT TATACTGGAA AAACTCAGAG AACAGCTCAT TAAAGCAAAA
    GCATTTACGA TCAAGACGAT ACTACAAATC TATGTCCCGA TCAGACAGTT TTTTTACAAT
    CTTATACATC CTGAATACAG TGCTGTGACT GATGTCTATG TGCTGATGTT CCTGGCAGAC
    ACTGTAGATT TCATCATTAT TGTTTTTGGA TTTTGGGCGT TTGGAAAACA CTCCGCTGCA
    GCAGATATTA CATCCTCTCT ATCAGAAGAC CAAGTCCCAG AAGCATTTTT GGTAATGGTG
    CTTATCCAGT TTGGAACCAT GGTTGTTGAT CGTGCTTTGT ATCTTCGCAA AACAGTCATG
    GGAAAAGTTA TTTTCCAAGT TATCCTTGTA TTTGGAATCC ATTTTTGGAT GTTCTTCATC
    TTGCCAGGGG TAACAGAAAG GAAATTTAGT CAGAACACTG TTGCCCAGCT GTGGTATTTT
    GTCAAGTGCA TTTATTTTGG ATTGTCGGCA TATCAGATAC GTTGTGGATA TCCAACTCGG
    GTGCTTGGTA ACTTCCTTAC TAAAAGCTAC AATTATGTCA ACCTGTTCCT TTTCCAAGGC
    TTTCGCCTCG TTCCATTTCT TACGGAGCTG CGAGCTGTTA TGGACTGGGT ATGGACTGAC
    ACCACTCTCA GTCTTTCCAG CTGGATCTGT GTAGAGGACA TTTATGCACA TATATTTATA
    CTAAAATGCT GGCGGGAGTC AGAAAAGAGA TATCCCCAGC CACGAGGCCA AAAGAAGAAA
    AAGGTTGTGA AATACGGAAT GGGTGGAATG ATAATAGTTC TGCTGATTTG TATCGTGTGG
    TTTCCTTTGC TCTTCATGTC CTTGATTAAA TCAGTTGCTG GGGTCACTAA CAAGCCCCTA
    GATGTGTCCA TTACAATAAC CCTTGGAGGT TATCAGCCTA TTTTTACCAT GAGTGCACAA
    CAAAATCAGC TGCAAGGCTT GAATGACAAT GAATTTAAGC ATTTACATAA TATATATAAA
    GGCAATACAC CTGCCATGCA GTTTCTGGAA TCCTATATGC AGGAAGACAT CACCATAGCA
    GGACTGGAAG GCAATTCCAA CTCTCTGTGG ACCATCAGCC CTCCGAGCAG GACAATGATG
    ATAGAAAGAC TTATCCAGGA ACCTGACTTC ACAGCGGTCA TTTCTTGGTC CATTCGAAGA
    AATGTGAGTC TTGGAGCTAA GGCAGAGGTA ACAGGTGAAA AATATACAAT ATCACTGGCA
    GGTCAAACTC GGAGGGAACT TGCGGATATG ATGAACAGTT CATCTCCCAA TAAGACAGTG
    GAACTGATAA AGATATACCC ATTGTGTTTA AAAGCACCAA GTGATGTTAC TGCAAAACCC
    ATCCTTCAGC TTCTTAAGAA TCAAGAGTTT GTGAATATTA GCGTTTTACT AAGAAAAGAA
    AACACATCAG AAGGGGTTCG AGAATGGTGG GTAGTGAATC AAAGCCATAA AGAGTTCATT
    AAGAAGGATG GCCTGGAACT ATGGGTTTTC AGTGACAAAG TCAGTCCTCC AAGTCTTGGA
    TTCTTGGCAG GCTATGGAAT AATGGGACTA TATGCCTCTG TAGTTCTCGT AATAGGAAAG
    TTTGTGCGTG AGTTTTTCAG CGGAATTTCG CACTCCATCA TGTTTGAGGA GCTTCCCAAC
    GTTGACCGTA TACTGAAGCT GTGCACAGAC ATTTTCCTTG TGAGAGAGAC TGGAGAGTTG
    GAACTGGAGG AAGACCTGTA TGCCAAATTA ATATTTTTGT ACCGCTCCCC AGAAACTATG
    ATCAAATGGA CAAGAGAGAA AGATAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012820815.1
    CDS612..8981
    Translation

    Target ORF information:

    RefSeq Version XM_012965361.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis piezo-type mechanosensitive ion channel component 2 (piezo2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012965361.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGGCATCGG AAGTGGTGTG TGGGCTCATC TTCAGATTTC TTCTCCCACT ATGCCTGGTT 
    GTAGCATGCA TGTTCCGGTA CAATGGAATA TCTTTTGTCT ACCTCATCTA CCTCTTGCTT
    ATACCTTTAT TTTCGGAGCC TACCAAAATA ACAATGCAAG GTCATACAGG ACGGTTATTA
    AAATCCTTAT GTGTTTCCAG TCTGTCATTT CTGCTGCTGC ACATCATCTT TCAAATTACC
    GTATACAGCC TCGAGGCCAG AAAACACATT GAACCACATT TTAACTGTTC AACATGGGAG
    AAAGCAATAC GACAAATTGG TTTTGAAAGT GTAAAAGGAG CAGATGCTGG TAATGGAATC
    CGCGTGTTTG CACCAGATAT CGGAATGTTT ATTGCAAGCT TGGCTACCCT GTTGGTTTGT
    AGAAAACTAG TTCAGAAAAC AGTTACTGAA GAGGACGCAC AATACAACCC CCAGTTTGTA
    AATGAAGAAA TGGCAGAAGA AGGAAAGGTG GACCTGGATG AAGCGTTAAT GTATGAGGAA
    GACTTTGATG GAGAAGAGGA TGAAATTGAG GCAGCTGAAG AAAGCACAAA ATTAAAAATT
    CTTCGCAGAA TTGCTTCATT TGCATCCGTA CTTAAAGAGT TTATTGGCAA CATTATAACC
    ACCGCTGGGA AAGTAGTTAT TACAATTTTA CTTGGTTTAA CAGGCATGAT GTTGCCGTCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT TTGGGGCTAT GTACATGGTG GTCCTGTTGC
    CGAGTATTTG ATCCGCTGAT ATTCAGCTGT CTATGTGTAT TAATGGCTAT ATTTAGTGCA
    GGGCACTTAA TTGGACTTTA TCTGTACCAG TTGCAGTTTT TTCACGAGAT TATTCCACCA
    AATGATTTCT ATGCCAGGTT GTTTGGTGTT ACTTCATTAG TTCAAACCGA CTGCTGTAAC
    ACTTGGAGAT TTTTTGCAAA CAACAGTCTA CCATGGAACC AGTATACCAA TCCTGTCATG
    CTTTTAGTAC TGTATTACAC GCTGGCAACT CTCATACGCC TTTGGCTACA GGAACCTATT
    AATGTAGAGG AAGAGAAGGA GGAAGACTGT AGCCCAGCAG CACTTACTGC AGAAAGAAGA
    CGTAGTTTGT GGTACTCTAG CCACTACACA ACGGATGAAA GACAACTTCT GTCCATGACT
    CAAGATGACT ACAAGCCATC TGATGTTTTA CTAGTAACTG TAAATGGGAC TCCCGTAGAT
    TACTGTTCAC TAAATTCATC ACTTCCGATA GAAAATGGTC CAACTAAAAT TGACATGTAT
    TCAACACCGC AATATAAATG GGACCATGAA GATGAAACTT CAGAAAAGAA AGAAGAGGAA
    GAGCAAGAAG AAGAACCCAA TGAAGAGGTT GCAGAAGAGA AAGAAAACAC TAAAGTTCAT
    GTAATGGTGG TGGCATTTCA GTTCATTATG AAGCAAAGCT ATATATGTGC ACTAATTTCA
    ATGATGGCCT GGAGCATCAC ATACCACAGC TGGTTGACTT TTGTTTTGTT AATCTGGTCT
    TGTGCTCTCT GGATGGTCCG AAATAGGAGA AAATATGCTA TGCTTAGTTC ACCTTTTATG
    GTTATGTATG GTAATTTACT TTTAACATTA CAATACATAT GGAGCATAGC CTTACAAAGT
    GATGAACTTC CAGAAGTTAT GGGTCTCCTT GAGAGGAAAA AGCCCGAAGA GCTTGTATCG
    AAGATCATTT TCACAGTTAC ATTTTGGCTT TTGCTCAGAC AACACCTTAC AGAGCAAAAG
    GCATTAAAGC AAAAAGAATT AGCATTATCT GAAATAAAAG TTGGAAGTGA AGACAAAGAA
    GGTGAAGTGC AAGAAGAAGA AGAAGAAGTG GAGGATGAAA ATGATATCAT GAAGGTCCTC
    GGAAAGCTAG TGCAGGCTAT GTTTGTCAAG TACTGGATTT ATGTCTGTGG CGGCATGTTT
    TTTTTTGTCA GCTTTGAAGG AAAAATAGTT ATGTACAAAA TTATCTACAT GATGCTCTTC
    CTATTCTGTG TAGCTCTGTA TCAGGTGCAC TATGAATGGT GGAGACGAAT TTTAAAATAT
    TTTTGGATGT CTGTGGTTGT TTACACCATG TTGGTGCTTG TTTTTATTTA CACTTACCAA
    TTTAAATCAT TTCCTGGCCT TTGGATGAAT ATGACAGGGC TGGATAAGGA AAAGTTAGCA
    GATCTTGGTT TAGAGACATT TAACTTGACT GACCTATTTA CTCGTATATT TATCCCAACC
    TCATTTTTGT TAGTGTGCAT TTTGCACCTA CATTACTTCC ATGACCGTTT TCTCCAGCTC
    ACTGACCTAA AGGCTGTAAC CAGCAAGCAG GACAGCACAA TTTACAGCCA TGCCAAAGTA
    AATGGTCGAG TTTACTTAAT AATAAATAGC ATCAAGCAAA AAATTCCAAT TCAGCAAAAT
    GAATTGGTGC ATCCAGAAGG AAGTTTGCCT GATATAACGA TGATGAACTT AACAACAGAA
    TCTGAAATGC TTGAGGAAAA GCGGTTGGAA ATTTATGACG AAAAAAAAAC TCATGACCAA
    GACTCAGATG ATGAATGGGA TAAAGGGGAT AGCAAAGAAC CAGGTCATTC TGATGATGAA
    GCTGAGGATG AGGAATCAGA AGAAGAAGAA ACAACAACAG AGTCCAGGAA CAAGTGGCAT
    CTAGTGATAG ATCGCTTAAC GGTTCTTTTC CTAAAGTTTC TGGAATATTT TCACAAGTTT
    CAGGTCTTTG TTTGGTGGCT TCTGGAGCTT CACATGATAA AGATTGTTTC TTCATACATC
    ATTTGGCTTA CTATAAAGGA GGTTTCACTG CTCAATTATG TATTTTTTAT TGCCTGGGCC
    TTCGCTTTGC CCTACTCACA ATTCCGGCCA TTGGCATCAA GTGTTTGCAC AGTCTGGACA
    TGTGTCATCA TCATATGTAA AATGTTGTAC CAGCTAGCCT CCATTGACCC TGCAACCTTT
    TCCAGCAACT GTTTAATGCC ACAGCTAAAC GAAACAACTG CACGTAACCA AACTGAGCTT
    AATTCTTCTG TGCTTTACAA TTCCTCTGTT GATCCTTCTA ATTGGGTTGG ACTAAAGAAG
    TCAACTCCAC TCCTTGCTTA TTTGCGGAAC AATTTGCTGA TGTTGGCCAT GTTAGCTTTT
    GAAGTAACCA TTTACCGGCA TCAGGAGTAT TACAGGTTGC GAAACAATCT CACCACACCT
    GCAACAAAAA CCATCTTTCA TGACATCACA AGACTTCACT TGGATGATGG ACTCATAAAT
    AGTGCCAAGT ATTTTGTGAA CTACTTTTTC TACAAGTTTG GATTGGAAAC ATGTTTCTTA
    CTGTCGGTAA ATGTAATCGG TCAGAGGATG GACTTTTTTG CCATGGTTCA TGCACTTTGG
    CTTTTTGGCG TATTATACCG ACGCAGAAGG AAAGCGATTG CAGAAACCTG GCACAGATAT
    TGCTGTTTTC TTGCTTGCAT TATGGCATTC CAGTATTTTA TATGTATTGG CATCCCCCCT
    GCTGCATGTA AAGACTATCC TTGGAGACAA CCTGGAGCCA ATTTTAATTC TAACATAATA
    AAGTGGTTAT ACTTTCCAGA CTTTATTGTG AGGCCTAGTC CTCTGTTTCT TGTCTATGAT
    TTTTTGCTTT TGCTTTGTGC GTCCCTTCAA AGAGAAGTGT TTGAGGAAGA AAATAAGGCA
    GCAGTTCGAA TCATGGCTGG TGACAATGTA GAAATTTGCA TGAACCTTGA TGCTGCATCT
    TTTAGCCAAC ATAATCCTGT ACCTGATTTC ATATACTGCA GATCTTATTT GGACATGGGT
    AAAGTTATAA TGTTTAGTTA CCTTTTCTGG TTTGTGCTCA CCATCATCTT CATCACGGGA
    ACTACACGGA TCAGCATATT CTGCATGGGC TACTTGGTAG CTTGCTTCTA TTTTCTATTA
    TTTGGTGGAG ATTTGCTGTT AAAACCTATA AGAATCATCC TACGCTATTG GGATTGGCTG
    ATTGCTTACA ATGTTTTTGT CATTACAATG AAAAATGTAT TATCAATAGG AGCATGTGGC
    TACATTGAAT ATTTAATCAA AGACAGTTGT TGGTTAATTC AGGCATTCAG TCTTTCTTGT
    ACAGTGAAAG GTTATTTTAT CCCCCAAAAC ACACCTGACT GTCAGTTGCC AAGCGGAGAG
    GCTGGAATCA TTTGGGACAG TATATGTTTT GCTTTCCTTT TATTGCAAAG AAGAGTGTTT
    ATGAGCTATT ACTTCTTACA TGTGGTTGCA GACATCAAAT CATCTCAGAT TCTAGCATCA
    AGAGGTGCTG AACTGTTCCA AGCTACAATT GTAAAAGCTG TAAAAGCAAG AATTGAAGAA
    GAAAAAAAAT CTATGGACCA GCTCAAGAGA CAGATGGACC ACATTAAAGC CAGGCAAGAA
    AAATATAAAA AAGGTAAAGA AAAAATGCTG AGTCTTACAC AGGATGGAGA AGGACAAGAA
    GTTCAAAAAC TAGATGATGA CGATGAAGAA GGAGACAAAG CAGACAAAGA GAAAGCCAAG
    GGCAAAAAAA AGCAGTGGTG GCGACCTTGG GTTGATCATG CTTCCATGGT GAAAAGCGGT
    GATTATTATT TGTTTGAGAC GGACAGTGAA GAAGAAGATG AGGATGACAA AAAAGAAGAA
    GAGCCTAGAA GAAAATCTGC CTTCCAGTTT TTGTACCAAG CATGGATTAC TGATCCAAAA
    ACAGCCCTTA AGCAAAGAAG CATGGAAGCC AAAGAAGAAA AGGCAAGGGA AATGAGGCGA
    AAGGGCATTG CTGAAGGTGA TATTATTGTA GAATGTGGGG ATAAAGAAGA AGAAAAAGAG
    CAAGGAAAAA AGAAATCAGA TGGCCCAGAC AATATCTTCA AAAGAATATT TAACATCCTG
    AAGTTTACTT GGGTCCTCTT CCTTGCTACA ATCGATAGTT TTACGGCTTG GCTAAACTCC
    ATTTCAAGAG AACATATTGA TATCTCCAAA GTTCTAAGCA TAGAACGTTG CATGCTGACT
    AGAGAGATAA AAAAGGGCAA TGTGCCTACT CGAGAAAGCA TCCACATGTA TTACCAAAAT
    CAAATAATGA TGAGCCTTTC TAGAGAGTCT GGACTTGACA GAATTGATGA CCAACCTGGT
    TCATCGTCCA GTCACCATAG CCATGACAAG ATGGCGAGTA AAAGGATGGA CAGTGCAGAC
    TCAGCTGTAT CTCATGATAG CATATCAAGC TGCTGCACTG AAGCAACCAT GCTATTCTCA
    AGGCAATCTA CACTTGATGA TTTAGATGGA CCAGACTCTG TACCAAAAAC AAGTGAGCGT
    GCTCGACCTA GGCTTCGTAA AATGTACAGC ATGGACATGT CTTCTTCATC TGCTGACAGT
    ATAGCTTCAA GTGAGCCAAC TCAGTGCACC ATGGTCTACT CACGGCAGGG CACAACAGAA
    ACTATTGAGG AAGTGGAAGA TGAAGAGGAG GAAGAAAGCA AAAGTCCTTT ATCAAAGATA
    GAAGCCGAGG AAGAAGAATA TGACATGGAT TTGGAAGGGA CAAGCTATAC TCCGGACACA
    GAATTTCCCC TGTACGCCAC TACAGAGGGG GATCTTCCTC CTTCGTACAG TAAAGCCGGC
    AGCTTCGAAC AACTTACATT TGTATCTCAG GACTCGGCTG ACAATTTAAT GGTTCTAAGC
    CCAGATGAAA GTCGTACTGA TAAGCTACAT GATCATATTC TTCCACCTCT TACACATGAA
    CTGACTGCTA GCGAGCTGCT TCTAAACAAA ATGTTTTATG ATGAAGAGTT AGAAGACTCT
    GAAAAATTTT ATACTACTCA ACCACGCCCG CTGCAGCTTA TTTATGCCTT GTATAATACA
    TTGGTGGCCA GATCAGAAAT GGTGTGCTAT TTTGTGATTA TTCTTAATCA CATGATTTCA
    GCTTCTATGA TCACATTGGT ACTGCCCATC CTTATATTCC TTTGGGCTAT GCTTTCTGTC
    CCAAGGCCCA GTAAGAGATT TTGGATGACA GCTATTGTAT ATACAGAGGT GACAATTGTT
    ATTAAATATT TTTTCCAATT TGGATTCTTC CCATGGAATA AAAATGTGGA TTATACAAAG
    GACAAACCTT ACTTCCCACC TAACATAATG GGCATTGAGA AGAAAGAAGG TTATGTGCAC
    TATGACCTGG TGCAACTGCT TGCTCTGTTT TTCCATAGAT CTATTTTAAA GTGTCATGGG
    CTTTGGGATG AAGATGATGT CACTGAAAAA GCCACAGATA AAGACGACAC GGATGGAGAG
    ATATCAGAGG CATCAGGAAG AAGGGATTCC GTTGGCTCAT TAAAATCTGT CAATCTAGCT
    GCGTCTGTGG AGTCAGTCCA TGTGCACTTC CCTGAGGAAA AGACCGCTAT CAGGAGAAAA
    AGCACAAGCA GTGGATCGCA AATTTCCCGA AGGTCAAGTT ATTCCTCGCA CAGGTCTAAA
    AGAGGCAGCA CCAGTACTCG GAACAGCAGT CAAAAAGGAA GCAGCATTCC CAGTATTAAA
    CTGAAATCTA GAAAGGAACT TATACTGGAA AAACTCAGAG AACAGCTCAT TAAAGCAAAA
    GCATTTACGA TCAAGACGAT ACTACAAATC TATGTCCCGA TCAGACAGTT TTTTTACAAT
    CTTATACATC CTGAATACAG TGCTGTGACT GATGTCTATG TGCTGATGTT CCTGGCAGAC
    ACTGTAGATT TCATCATTAT TGTTTTTGGA TTTTGGGCGT TTGGAAAACA CTCCGCTGCA
    GCAGATATTA CATCCTCTCT ATCAGAAGAC CAAGTCCCAG AAGCATTTTT GGTAATGGTG
    CTTATCCAGT TTGGAACCAT GGTTGTTGAT CGTGCTTTGT ATCTTCGCAA AACAGTCATG
    GGAAAAGTTA TTTTCCAAGT TATCCTTGTA TTTGGAATCC ATTTTTGGAT GTTCTTCATC
    TTGCCAGGGG TAACAGAAAG GAAATTTAGT CAGAACACTG TTGCCCAGCT GTGGTATTTT
    GTCAAGTGCA TTTATTTTGG ATTGTCGGCA TATCAGATAC GTTGTGGATA TCCAACTCGG
    GTGCTTGGTA ACTTCCTTAC TAAAAGCTAC AATTATGTCA ACCTGTTCCT TTTCCAAGGC
    TTTCGCCTCG TTCCATTTCT TACGGAGCTG CGAGCTGTTA TGGACTGGGT ATGGACTGAC
    ACCACTCTCA GTCTTTCCAG CTGGATCTGT GTAGAGGACA TTTATGCACA TATATTTATA
    CTAAAATGCT GGCGGGAGTC AGAAAAGAGA TATCCCCAGC CACGAGGCCA AAAGAAGAAA
    AAGGTTGTGA AATACGGAAT GGGTGGAATG ATAATAGTTC TGCTGATTTG TATCGTGTGG
    TTTCCTTTGC TCTTCATGTC CTTGATTAAA TCAGTTGCTG GGGTCACTAA CAAGCCCCTA
    GATGTGTCCA TTACAATAAC CCTTGGAGGT TATCAGCCTA TTTTTACCAT GAGTGCACAA
    CAAAATCAGC TGCAAGGCTT GAATGACAAT GAATTTAAGC ATTTACATAA TATATATAAA
    GGCAATACAC CTGCCATGCA GTTTCTGGAA TCCTATATGC AGGAAGACAT CACCATAGCA
    GGACTGGAAG GCAATTCCAA CTCTCTGTGG ACCATCAGCC CTCCGAGCAG GACAATGATG
    ATAGAAAGAC TTATCCAGGA ACCTGACTTC ACAGCGGTCA TTTCTTGGTC CATTCGAAGA
    AATGTGAGTC TTGGAGCTAA GGCAGAGGTA ACAGGTGAAA AATATACAAT ATCACTGGCA
    GGTCAAACTC GGAGGGAACT TGCGGATATG ATGAACAGTT CATCTCCCAA TAAGACAGTG
    GAACTGATAA AGATATACCC ATTGTGTTTA AAAGCACCAA GTGATGTTAC TGCAAAACCC
    ATCCTTCAGC TTCTTAAGAA TCAAGAGTTT GTGAATATTA GCGTTTTACT AAGAAAAGAA
    AACACATCAG AAGGGGTTCG AGAATGGTGG GTAGTGAATC AAAGCCATAA AGAGTTCATT
    AAGAAGGATG GCCTGGAACT ATGGGTTTTC AGTGACAAAG TCAGTCCTCC AAGTCTTGGA
    TTCTTGGCAG GCTATGGAAT AATGGGACTA TATGCCTCTG TAGTTCTCGT AATAGGAAAG
    TTTGTGCGTG AGTTTTTCAG CGGAATTTCG CACTCCATCA TGTTTGAGGA GCTTCCCAAC
    GTTGACCGTA TACTGAAGCT GTGCACAGAC ATTTTCCTTG TGAGAGAGAC TGGAGAGTTG
    GAACTGGAGG AAGACCTGTA TGCCAAATTA ATATTTTTGT ACCGCTCCCC AGAAACTATG
    ATCAAATGGA CAAGAGAGAA AGATAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa35456
    Clone ID Related Accession (Same CDS sequence) XM_012965362.2
    Accession Version XM_012965362.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8307bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product piezo-type mechanosensitive ion channel component 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012965362.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    ATGGCATCGG AAGTGGTGTG TGGGCTCATC TTCAGATTTC TTCTCCCACT ATGCCTGGTT 
    GTAGCATGCA TGTTCCGGTA CAATGGAATA TCTTTTGTCT ACCTCATCTA CCTCTTGCTT
    ATACCTTTAT TTTCGGAGCC TACCAAAATA ACAATGCAAG GTCATACAGG ACGGTTATTA
    AAATCCTTAT GTGTTTCCAG TCTGTCATTT CTGCTGCTGC ACATCATCTT TCAAATTACC
    GTATACAGCC TCGAGGCCAG AAAACACATT GAACCACATT TTAACTGTTC AACATGGGAG
    AAAGCAATAC GACAAATTGG TTTTGAAAGT GTAAAAGGAG CAGATGCTGG TAATGGAATC
    CGCGTGTTTG CACCAGATAT CGGAATGTTT ATTGCAAGCT TGGCTACCCT GTTGGTTTGT
    AGAAAACTAG TTCAGAAAAC AGTTACTGAA GAGGACGCAC AATACAACCC CCAGTTTGTA
    AATGAAGAAA TGGCAGAAGA AGGAAAGGTG GACCTGGATG AAGCGTTAAT GTATGAGGAA
    GACTTTGATG GAGAAGAGGA TGAAATTGAG GCAGCTGAAG AAAGCACAAA ATTAAAAATT
    CTTCGCAGAA TTGCTTCATT TGCATCCGTA CTTAAAGAGT TTATTGGCAA CATTATAACC
    ACCGCTGGGA AAGTAGTTAT TACAATTTTA CTTGGTTTAA CAGGCATGAT GTTGCCGTCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT TTGGGGCTAT GTACATGGTG GTCCTGTTGC
    CGAGTATTTG ATCCGCTGAT ATTCAGCTGT CTATGTGTAT TAATGGCTAT ATTTAGTGCA
    GGGCACTTAA TTGGACTTTA TCTGTACCAG TTGCAGTTTT TTCACGAGAT TATTCCACCA
    AATGATTTCT ATGCCAGGTT GTTTGGTGTT ACTTCATTAG TTCAAACCGA CTGCTGTAAC
    ACTTGGAGAT TTTTTGCAAA CAACAGTCTA CCATGGAACC AGTATACCAA TCCTGTCATG
    CTTTTAGTAC TGTATTACAC GCTGGCAACT CTCATACGCC TTTGGCTACA GGAACCTATT
    AATGTAGAGG AAGAGAAGGA GGAAGACTGT AGCCCAGCAG CACTTACTGC AGAAAGAAGA
    CGTAGTTTGT GGTACTCTAG CCACTACACA ACGGATGAAA GACAACTTCT GTCCATGACT
    CAAGATGACT ACAAGCCATC TGATGTTTTA CTAGTAACTG TAAATGGGAC TCCCGTAGAT
    TACTGTTCAC TAAATTCATC ACTTCCGATA GAAAATGGTC CAACTAAAAT TGACATGTAT
    TCAACACCGC AATATAAATG GGACCATGAA GATGAAACTT CAGAAAAGAA AGAAGAGGAA
    GAGCAAGAAG AAGAACCCAA TGAAGAGGTT GCAGAAGAGA AAGAAAACAC TAAAGTTCAT
    GTAATGGTGG TGGCATTTCA GTTCATTATG AAGCAAAGCT ATATATGTGC ACTAATTTCA
    ATGATGGCCT GGAGCATCAC ATACCACAGC TGGTTGACTT TTGTTTTGTT AATCTGGTCT
    TGTGCTCTCT GGATGGTCCG AAATAGGAGA AAATATGCTA TGCTTAGTTC ACCTTTTATG
    GTTATGTATG GTAATTTACT TTTAACATTA CAATACATAT GGAGCATAGC CTTACAAAGT
    GATGAACTTC CAGAAGTTAT GGGTCTCCTT GAGAGGAAAA AGCCCGAAGA GCTTGTATCG
    AAGATCATTT TCACAGTTAC ATTTTGGCTT TTGCTCAGAC AACACCTTAC AGAGCAAAAG
    GCATTAAAGC AAAAAGAATT AGCATTATCT GAAATAAAAG TTGGAAGTGA AGACAAAGAA
    GGTGAAGTGC AAGAAGAAGA AGAAGAAGTG GAGGATGAAA ATGATATCAT GAAGGTCCTC
    GGAAAGCTAG TGCAGGCTAT GTTTGTCAAG TACTGGATTT ATGTCTGTGG CGGCATGTTT
    TTTTTTGTCA GCTTTGAAGG AAAAATAGTT ATGTACAAAA TTATCTACAT GATGCTCTTC
    CTATTCTGTG TAGCTCTGTA TCAGGTGCAC TATGAATGGT GGAGACGAAT TTTAAAATAT
    TTTTGGATGT CTGTGGTTGT TTACACCATG TTGGTGCTTG TTTTTATTTA CACTTACCAA
    TTTAAATCAT TTCCTGGCCT TTGGATGAAT ATGACAGGGC TGGATAAGGA AAAGTTAGCA
    GATCTTGGTT TAGAGACATT TAACTTGACT GACCTATTTA CTCGTATATT TATCCCAACC
    TCATTTTTGT TAGTGTGCAT TTTGCACCTA CATTACTTCC ATGACCGTTT TCTCCAGCTC
    ACTGACCTAA AGGCTGTAAC CAGCAAGCAG GACAGCACAA TTTACAGCCA TGCCAAAGTA
    AATGGTCGAG TTTACTTAAT AATAAATAGC ATCAAGCAAA AAATTCCAAT TCAGCAAAAT
    GAATTGGTGC ATCCAGAAGG AAGTTTGCCT GATATAACGA TGATGAACTT AACAACAGAA
    TCTGAAATGC TTGAGGAAAA GCGGTTGGAA ATTTATGACG AAAAAAAAAC TCATGACCAA
    GACTCAGATG ATGAATGGGA TAAAGGGGAT AGCAAAGAAC CAGGTCATTC TGATGATGAA
    GCTGAGGATG AGGAATCAGA AGAAGAAGAA ACAACAACAG AGTCCAGGAA CAAGTGGCAT
    CTAGTGATAG ATCGCTTAAC GGTTCTTTTC CTAAAGTTTC TGGAATATTT TCACAAGTTT
    CAGGTCTTTG TTTGGTGGCT TCTGGAGCTT CACATGATAA AGATTGTTTC TTCATACATC
    ATTTGGCTTA CTATAAAGGA GGTTTCACTG CTCAATTATG TATTTTTTAT TGCCTGGGCC
    TTCGCTTTGC CCTACTCACA ATTCCGGCCA TTGGCATCAA GTGTTTGCAC AGTCTGGACA
    TGTGTCATCA TCATATGTAA AATGTTGTAC CAGCTAGCCT CCATTGACCC TGCAACCTTT
    TCCAGCAACT GTTTAATGCC ACAGCTAAAC GAAACAACTG CACGTAACCA AACTGAGCTT
    AATTCTTCTG TGCTTTACAA TTCCTCTGTT GATCCTTCTA ATTGGGTTGG ACTAAAGAAG
    TCAACTCCAC TCCTTGCTTA TTTGCGGAAC AATTTGCTGA TGTTGGCCAT GTTAGCTTTT
    GAAGTAACCA TTTACCGGCA TCAGGAGTAT TACAGGTTGC GAAACAATCT CACCACACCT
    GCAACAAAAA CCATCTTTCA TGACATCACA AGACTTCACT TGGATGATGG ACTCATAAAT
    AGTGCCAAGT ATTTTGTGAA CTACTTTTTC TACAAGTTTG GATTGGAAAC ATGTTTCTTA
    CTGTCGGTAA ATGTAATCGG TCAGAGGATG GACTTTTTTG CCATGGTTCA TGCACTTTGG
    CTTTTTGGCG TATTATACCG ACGCAGAAGG AAAGCGATTG CAGAAACCTG GCACAGATAT
    TGCTGTTTTC TTGCTTGCAT TATGGCATTC CAGTATTTTA TATGTATTGG CATCCCCCCT
    GCTGCATGTA AAGACTATCC TTGGAGACAA CCTGGAGCCA ATTTTAATTC TAACATAATA
    AAGTGGTTAT ACTTTCCAGA CTTTATTGTG AGGCCTAGTC CTCTGTTTCT TGTCTATGAT
    TTTTTGCTTT TGCTTTGTGC GTCCCTTCAA AGAGAAGTGT TTGAGGAAGA AAATAAGGCA
    GCAGTTCGAA TCATGGCTGG TGACAATGTA GAAATTTGCA TGAACCTTGA TGCTGCATCT
    TTTAGCCAAC ATAATCCTGT ACCTGATTTC ATATACTGCA GATCTTATTT GGACATGGGT
    AAAGTTATAA TGTTTAGTTA CCTTTTCTGG TTTGTGCTCA CCATCATCTT CATCACGGGA
    ACTACACGGA TCAGCATATT CTGCATGGGC TACTTGGTAG CTTGCTTCTA TTTTCTATTA
    TTTGGTGGAG ATTTGCTGTT AAAACCTATA AGAATCATCC TACGCTATTG GGATTGGCTG
    ATTGCTTACA ATGTTTTTGT CATTACAATG AAAAATGTAT TATCAATAGG AGCATGTGGC
    TACATTGAAT ATTTAATCAA AGACAGTTGT TGGTTAATTC AGGCATTCAG TCTTTCTTGT
    ACAGTGAAAG GTTATTTTAT CCCCCAAAAC ACACCTGACT GTCAGTTGCC AAGCGGAGAG
    GCTGGAATCA TTTGGGACAG TATATGTTTT GCTTTCCTTT TATTGCAAAG AAGAGTGTTT
    ATGAGCTATT ACTTCTTACA TGTGGTTGCA GACATCAAAT CATCTCAGAT TCTAGCATCA
    AGAGGTGCTG AACTGTTCCA AGCTACAATT GTAAAAGCTG TAAAAGCAAG AATTGAAGAA
    GAAAAAAAAT CTATGGACCA GCTCAAGAGA CAGATGGACC ACATTAAAGC CAGGCAAGAA
    AAATATAAAA AAGGTAAAGA AAAAATGCTG AGTCTTACAC AGGATGGAGA AGGACAAGAA
    GTTCAAAAAC TAGATGATGA CGATGAAGAA GGAGACAAAG CAGACAAAGA GAAAGCCAAG
    GGCAAAAAAA AGCAGTGGTG GCGACCTTGG GTTGATCATG CTTCCATGGT GAAAAGCGGT
    GATTATTATT TGTTTGAGAC GGACAGTGAA GAAGAAGATG AGGATGACAA AAAAGAAGAA
    GAGCCTAGAA GAAAATCTGC CTTCCAGAGA GCTATTGGAA AGTTTGCATC AGCCATTATA
    GCCTTGCCAA AGTCTGTCAT TAAACTGCCT AAAACAATTC TTCAATATTT AATCAGAGCT
    GCAAAGTTTT TGTACCAAGC ATGGATTACT GATCCAAAAA CAGCCCTTAA GCAAAGAAGC
    ATGGAAGCCA AAGAAGAAAA GGCAAGGGAA ATGAGGCGAA AGGGCATTGC TGAAGGTGAT
    ATTATTGTAG AATGTGGGGA TAAAGAAGAA GAAAAAGAGC AAGGAAAAAA GAAATCAGAT
    GGCCCAGACA ATATCTTCAA AAGAATATTT AACATCCTGA AGTTTACTTG GGTCCTCTTC
    CTTGCTACAA TCGATAGTTT TACGGCTTGG CTAAACTCCA TTTCAAGAGA ACATATTGAT
    ATCTCCAAAG TTCTAAGCAT AGAACGTTGC ATGCTGACTA GAGAGATAAA AAAGGGCAAT
    GTGCCTACTC GAGAAAGCAT CCACATGTAT TACCAAAATC AAATAATGAT GAGCCTTTCT
    AGAGAGTCTG GACTTGACAG AATTGATGAC CAACCTGGTT CATCGTCCAG TCACCATAGC
    CATGACAAGA TGGCGAGTAA AAGGATGGAC AGTGCAGACT CAGCTGTATC TCATGATAGC
    ATATCAAGTG AGCCAACTCA GTGCACCATG GTCTACTCAC GGCAGGGCAC AACAGAAACT
    ATTGAGGAAG TGGAAGATGA AGAGGAGGAA GAAAGCAAAA GTCCTTTATC AAAGATAGAA
    GCCGAGGAAG AAGAATATGA CATGGATTTG GAAGGGACAA GCTATACTCC GGACACAGAA
    TTTCCCCTGT ACGCCACTAC AGAGGGGGAT CTTCCTCCTT CGTACAGTAA AGCCGGCAGC
    TTCGAACAAC TTACATTTGT ATCTCAGGAC TCGGCTGACA ATTTAATGGT TCTAAGCCCA
    GATGAAAGTC GTACTGATAA GCTACATGAT CATATTCTTC CACCTCTTAC ACATGAACTG
    ACTGCTAGCG AGCTGCTTCT AAACAAAATG TTTTATGATG AAGAGTTAGA AGACTCTGAA
    AAATTTTATA CTACTCAACC ACGCCCGCTG CAGCTTATTT ATGCCTTGTA TAATACATTG
    GTGGCCAGAT CAGAAATGGT GTGCTATTTT GTGATTATTC TTAATCACAT GATTTCAGCT
    TCTATGATCA CATTGGTACT GCCCATCCTT ATATTCCTTT GGGCTATGCT TTCTGTCCCA
    AGGCCCAGTA AGAGATTTTG GATGACAGCT ATTGTATATA CAGAGGTGAC AATTGTTATT
    AAATATTTTT TCCAATTTGG ATTCTTCCCA TGGAATAAAA ATGTGGATTA TACAAAGGAC
    AAACCTTACT TCCCACCTAA CATAATGGGC ATTGAGAAGA AAGAAGGTTA TGTGCACTAT
    GACCTGGTGC AACTGCTTGC TCTGTTTTTC CATAGATCTA TTTTAAAGTG TCATGGGCTT
    TGGGATGAAG ATGATGTCAC TGAAAAAGCC ACAGATAAAG ACGACACGGA TGGAGAGATA
    TCAGAGGCAT CAGGAAGAAG GGATTCCGTT GGCTCATTAA AATCTGTCAA TCTAGCTGCG
    TCTGTGGAGT CAGTCCATGT GCACTTCCCT GAGGAAAAGA CCGCTATCAG GAGAAAAAGC
    ACAAGCAGTG GATCGCAAAT TTCCCGAAGG TCAAGTTATT CCTCGCACAG GTCTAAAAGA
    GGCAGCACCA GTACTCGGAA CAGCAGTCAA AAAGGAAGCA GCATTCCCAG TATTAAACTG
    AAATCTAGAA AGGAACTTAT ACTGGAAAAA CTCAGAGAAC AGCTCATTAA AGCAAAAGCA
    TTTACGATCA AGACGATACT ACAAATCTAT GTCCCGATCA GACAGTTTTT TTACAATCTT
    ATACATCCTG AATACAGTGC TGTGACTGAT GTCTATGTGC TGATGTTCCT GGCAGACACT
    GTAGATTTCA TCATTATTGT TTTTGGATTT TGGGCGTTTG GAAAACACTC CGCTGCAGCA
    GATATTACAT CCTCTCTATC AGAAGACCAA GTCCCAGAAG CATTTTTGGT AATGGTGCTT
    ATCCAGTTTG GAACCATGGT TGTTGATCGT GCTTTGTATC TTCGCAAAAC AGTCATGGGA
    AAAGTTATTT TCCAAGTTAT CCTTGTATTT GGAATCCATT TTTGGATGTT CTTCATCTTG
    CCAGGGGTAA CAGAAAGGAA ATTTAGTCAG AACACTGTTG CCCAGCTGTG GTATTTTGTC
    AAGTGCATTT ATTTTGGATT GTCGGCATAT CAGATACGTT GTGGATATCC AACTCGGGTG
    CTTGGTAACT TCCTTACTAA AAGCTACAAT TATGTCAACC TGTTCCTTTT CCAAGGCTTT
    CGCCTCGTTC CATTTCTTAC GGAGCTGCGA GCTGTTATGG ACTGGGTATG GACTGACACC
    ACTCTCAGTC TTTCCAGCTG GATCTGTGTA GAGGACATTT ATGCACATAT ATTTATACTA
    AAATGCTGGC GGGAGTCAGA AAAGAGATAT CCCCAGCCAC GAGGCCAAAA GAAGAAAAAG
    GTTGTGAAAT ACGGAATGGG TGGAATGATA ATAGTTCTGC TGATTTGTAT CGTGTGGTTT
    CCTTTGCTCT TCATGTCCTT GATTAAATCA GTTGCTGGGG TCACTAACAA GCCCCTAGAT
    GTGTCCATTA CAATAACCCT TGGAGGTTAT CAGCCTATTT TTACCATGAG TGCACAACAA
    AATCAGCTGC AAGGCTTGAA TGACAATGAA TTTAAGCATT TACATAATAT ATATAAAGGC
    AATACACCTG CCATGCAGTT TCTGGAATCC TATATGCAGG AAGACATCAC CATAGCAGGA
    CTGGAAGGCA ATTCCAACTC TCTGTGGACC ATCAGCCCTC CGAGCAGGAC AATGATGATA
    GAAAGACTTA TCCAGGAACC TGACTTCACA GCGGTCATTT CTTGGTCCAT TCGAAGAAAT
    GTGAGTCTTG GAGCTAAGGC AGAGGTAACA GGTGAAAAAT ATACAATATC ACTGGCAGGT
    CAAACTCGGA GGGAACTTGC GGATATGATG AACAGTTCAT CTCCCAATAA GACAGTGGAA
    CTGATAAAGA TATACCCATT GTGTTTAAAA GCACCAAGTG ATGTTACTGC AAAACCCATC
    CTTCAGCTTC TTAAGAATCA AGAGTTTGTG AATATTAGCG TTTTACTAAG AAAAGAAAAC
    ACATCAGAAG GGGTTCGAGA ATGGTGGGTA GTGAATCAAA GCCATAAAGA GTTCATTAAG
    AAGGATGGCC TGGAACTATG GGTTTTCAGT GACAAAGTCA GTCCTCCAAG TCTTGGATTC
    TTGGCAGGCT ATGGAATAAT GGGACTATAT GCCTCTGTAG TTCTCGTAAT AGGAAAGTTT
    GTGCGTGAGT TTTTCAGCGG AATTTCGCAC TCCATCATGT TTGAGGAGCT TCCCAACGTT
    GACCGTATAC TGAAGCTGTG CACAGACATT TTCCTTGTGA GAGAGACTGG AGAGTTGGAA
    CTGGAGGAAG ACCTGTATGC CAAATTAATA TTTTTGTACC GCTCCCCAGA AACTATGATC
    AAATGGACAA GAGAGAAAGA TAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012820816.1
    CDS612..8918
    Translation

    Target ORF information:

    RefSeq Version XM_012965362.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis piezo-type mechanosensitive ion channel component 2 (piezo2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012965362.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    ATGGCATCGG AAGTGGTGTG TGGGCTCATC TTCAGATTTC TTCTCCCACT ATGCCTGGTT 
    GTAGCATGCA TGTTCCGGTA CAATGGAATA TCTTTTGTCT ACCTCATCTA CCTCTTGCTT
    ATACCTTTAT TTTCGGAGCC TACCAAAATA ACAATGCAAG GTCATACAGG ACGGTTATTA
    AAATCCTTAT GTGTTTCCAG TCTGTCATTT CTGCTGCTGC ACATCATCTT TCAAATTACC
    GTATACAGCC TCGAGGCCAG AAAACACATT GAACCACATT TTAACTGTTC AACATGGGAG
    AAAGCAATAC GACAAATTGG TTTTGAAAGT GTAAAAGGAG CAGATGCTGG TAATGGAATC
    CGCGTGTTTG CACCAGATAT CGGAATGTTT ATTGCAAGCT TGGCTACCCT GTTGGTTTGT
    AGAAAACTAG TTCAGAAAAC AGTTACTGAA GAGGACGCAC AATACAACCC CCAGTTTGTA
    AATGAAGAAA TGGCAGAAGA AGGAAAGGTG GACCTGGATG AAGCGTTAAT GTATGAGGAA
    GACTTTGATG GAGAAGAGGA TGAAATTGAG GCAGCTGAAG AAAGCACAAA ATTAAAAATT
    CTTCGCAGAA TTGCTTCATT TGCATCCGTA CTTAAAGAGT TTATTGGCAA CATTATAACC
    ACCGCTGGGA AAGTAGTTAT TACAATTTTA CTTGGTTTAA CAGGCATGAT GTTGCCGTCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT TTGGGGCTAT GTACATGGTG GTCCTGTTGC
    CGAGTATTTG ATCCGCTGAT ATTCAGCTGT CTATGTGTAT TAATGGCTAT ATTTAGTGCA
    GGGCACTTAA TTGGACTTTA TCTGTACCAG TTGCAGTTTT TTCACGAGAT TATTCCACCA
    AATGATTTCT ATGCCAGGTT GTTTGGTGTT ACTTCATTAG TTCAAACCGA CTGCTGTAAC
    ACTTGGAGAT TTTTTGCAAA CAACAGTCTA CCATGGAACC AGTATACCAA TCCTGTCATG
    CTTTTAGTAC TGTATTACAC GCTGGCAACT CTCATACGCC TTTGGCTACA GGAACCTATT
    AATGTAGAGG AAGAGAAGGA GGAAGACTGT AGCCCAGCAG CACTTACTGC AGAAAGAAGA
    CGTAGTTTGT GGTACTCTAG CCACTACACA ACGGATGAAA GACAACTTCT GTCCATGACT
    CAAGATGACT ACAAGCCATC TGATGTTTTA CTAGTAACTG TAAATGGGAC TCCCGTAGAT
    TACTGTTCAC TAAATTCATC ACTTCCGATA GAAAATGGTC CAACTAAAAT TGACATGTAT
    TCAACACCGC AATATAAATG GGACCATGAA GATGAAACTT CAGAAAAGAA AGAAGAGGAA
    GAGCAAGAAG AAGAACCCAA TGAAGAGGTT GCAGAAGAGA AAGAAAACAC TAAAGTTCAT
    GTAATGGTGG TGGCATTTCA GTTCATTATG AAGCAAAGCT ATATATGTGC ACTAATTTCA
    ATGATGGCCT GGAGCATCAC ATACCACAGC TGGTTGACTT TTGTTTTGTT AATCTGGTCT
    TGTGCTCTCT GGATGGTCCG AAATAGGAGA AAATATGCTA TGCTTAGTTC ACCTTTTATG
    GTTATGTATG GTAATTTACT TTTAACATTA CAATACATAT GGAGCATAGC CTTACAAAGT
    GATGAACTTC CAGAAGTTAT GGGTCTCCTT GAGAGGAAAA AGCCCGAAGA GCTTGTATCG
    AAGATCATTT TCACAGTTAC ATTTTGGCTT TTGCTCAGAC AACACCTTAC AGAGCAAAAG
    GCATTAAAGC AAAAAGAATT AGCATTATCT GAAATAAAAG TTGGAAGTGA AGACAAAGAA
    GGTGAAGTGC AAGAAGAAGA AGAAGAAGTG GAGGATGAAA ATGATATCAT GAAGGTCCTC
    GGAAAGCTAG TGCAGGCTAT GTTTGTCAAG TACTGGATTT ATGTCTGTGG CGGCATGTTT
    TTTTTTGTCA GCTTTGAAGG AAAAATAGTT ATGTACAAAA TTATCTACAT GATGCTCTTC
    CTATTCTGTG TAGCTCTGTA TCAGGTGCAC TATGAATGGT GGAGACGAAT TTTAAAATAT
    TTTTGGATGT CTGTGGTTGT TTACACCATG TTGGTGCTTG TTTTTATTTA CACTTACCAA
    TTTAAATCAT TTCCTGGCCT TTGGATGAAT ATGACAGGGC TGGATAAGGA AAAGTTAGCA
    GATCTTGGTT TAGAGACATT TAACTTGACT GACCTATTTA CTCGTATATT TATCCCAACC
    TCATTTTTGT TAGTGTGCAT TTTGCACCTA CATTACTTCC ATGACCGTTT TCTCCAGCTC
    ACTGACCTAA AGGCTGTAAC CAGCAAGCAG GACAGCACAA TTTACAGCCA TGCCAAAGTA
    AATGGTCGAG TTTACTTAAT AATAAATAGC ATCAAGCAAA AAATTCCAAT TCAGCAAAAT
    GAATTGGTGC ATCCAGAAGG AAGTTTGCCT GATATAACGA TGATGAACTT AACAACAGAA
    TCTGAAATGC TTGAGGAAAA GCGGTTGGAA ATTTATGACG AAAAAAAAAC TCATGACCAA
    GACTCAGATG ATGAATGGGA TAAAGGGGAT AGCAAAGAAC CAGGTCATTC TGATGATGAA
    GCTGAGGATG AGGAATCAGA AGAAGAAGAA ACAACAACAG AGTCCAGGAA CAAGTGGCAT
    CTAGTGATAG ATCGCTTAAC GGTTCTTTTC CTAAAGTTTC TGGAATATTT TCACAAGTTT
    CAGGTCTTTG TTTGGTGGCT TCTGGAGCTT CACATGATAA AGATTGTTTC TTCATACATC
    ATTTGGCTTA CTATAAAGGA GGTTTCACTG CTCAATTATG TATTTTTTAT TGCCTGGGCC
    TTCGCTTTGC CCTACTCACA ATTCCGGCCA TTGGCATCAA GTGTTTGCAC AGTCTGGACA
    TGTGTCATCA TCATATGTAA AATGTTGTAC CAGCTAGCCT CCATTGACCC TGCAACCTTT
    TCCAGCAACT GTTTAATGCC ACAGCTAAAC GAAACAACTG CACGTAACCA AACTGAGCTT
    AATTCTTCTG TGCTTTACAA TTCCTCTGTT GATCCTTCTA ATTGGGTTGG ACTAAAGAAG
    TCAACTCCAC TCCTTGCTTA TTTGCGGAAC AATTTGCTGA TGTTGGCCAT GTTAGCTTTT
    GAAGTAACCA TTTACCGGCA TCAGGAGTAT TACAGGTTGC GAAACAATCT CACCACACCT
    GCAACAAAAA CCATCTTTCA TGACATCACA AGACTTCACT TGGATGATGG ACTCATAAAT
    AGTGCCAAGT ATTTTGTGAA CTACTTTTTC TACAAGTTTG GATTGGAAAC ATGTTTCTTA
    CTGTCGGTAA ATGTAATCGG TCAGAGGATG GACTTTTTTG CCATGGTTCA TGCACTTTGG
    CTTTTTGGCG TATTATACCG ACGCAGAAGG AAAGCGATTG CAGAAACCTG GCACAGATAT
    TGCTGTTTTC TTGCTTGCAT TATGGCATTC CAGTATTTTA TATGTATTGG CATCCCCCCT
    GCTGCATGTA AAGACTATCC TTGGAGACAA CCTGGAGCCA ATTTTAATTC TAACATAATA
    AAGTGGTTAT ACTTTCCAGA CTTTATTGTG AGGCCTAGTC CTCTGTTTCT TGTCTATGAT
    TTTTTGCTTT TGCTTTGTGC GTCCCTTCAA AGAGAAGTGT TTGAGGAAGA AAATAAGGCA
    GCAGTTCGAA TCATGGCTGG TGACAATGTA GAAATTTGCA TGAACCTTGA TGCTGCATCT
    TTTAGCCAAC ATAATCCTGT ACCTGATTTC ATATACTGCA GATCTTATTT GGACATGGGT
    AAAGTTATAA TGTTTAGTTA CCTTTTCTGG TTTGTGCTCA CCATCATCTT CATCACGGGA
    ACTACACGGA TCAGCATATT CTGCATGGGC TACTTGGTAG CTTGCTTCTA TTTTCTATTA
    TTTGGTGGAG ATTTGCTGTT AAAACCTATA AGAATCATCC TACGCTATTG GGATTGGCTG
    ATTGCTTACA ATGTTTTTGT CATTACAATG AAAAATGTAT TATCAATAGG AGCATGTGGC
    TACATTGAAT ATTTAATCAA AGACAGTTGT TGGTTAATTC AGGCATTCAG TCTTTCTTGT
    ACAGTGAAAG GTTATTTTAT CCCCCAAAAC ACACCTGACT GTCAGTTGCC AAGCGGAGAG
    GCTGGAATCA TTTGGGACAG TATATGTTTT GCTTTCCTTT TATTGCAAAG AAGAGTGTTT
    ATGAGCTATT ACTTCTTACA TGTGGTTGCA GACATCAAAT CATCTCAGAT TCTAGCATCA
    AGAGGTGCTG AACTGTTCCA AGCTACAATT GTAAAAGCTG TAAAAGCAAG AATTGAAGAA
    GAAAAAAAAT CTATGGACCA GCTCAAGAGA CAGATGGACC ACATTAAAGC CAGGCAAGAA
    AAATATAAAA AAGGTAAAGA AAAAATGCTG AGTCTTACAC AGGATGGAGA AGGACAAGAA
    GTTCAAAAAC TAGATGATGA CGATGAAGAA GGAGACAAAG CAGACAAAGA GAAAGCCAAG
    GGCAAAAAAA AGCAGTGGTG GCGACCTTGG GTTGATCATG CTTCCATGGT GAAAAGCGGT
    GATTATTATT TGTTTGAGAC GGACAGTGAA GAAGAAGATG AGGATGACAA AAAAGAAGAA
    GAGCCTAGAA GAAAATCTGC CTTCCAGAGA GCTATTGGAA AGTTTGCATC AGCCATTATA
    GCCTTGCCAA AGTCTGTCAT TAAACTGCCT AAAACAATTC TTCAATATTT AATCAGAGCT
    GCAAAGTTTT TGTACCAAGC ATGGATTACT GATCCAAAAA CAGCCCTTAA GCAAAGAAGC
    ATGGAAGCCA AAGAAGAAAA GGCAAGGGAA ATGAGGCGAA AGGGCATTGC TGAAGGTGAT
    ATTATTGTAG AATGTGGGGA TAAAGAAGAA GAAAAAGAGC AAGGAAAAAA GAAATCAGAT
    GGCCCAGACA ATATCTTCAA AAGAATATTT AACATCCTGA AGTTTACTTG GGTCCTCTTC
    CTTGCTACAA TCGATAGTTT TACGGCTTGG CTAAACTCCA TTTCAAGAGA ACATATTGAT
    ATCTCCAAAG TTCTAAGCAT AGAACGTTGC ATGCTGACTA GAGAGATAAA AAAGGGCAAT
    GTGCCTACTC GAGAAAGCAT CCACATGTAT TACCAAAATC AAATAATGAT GAGCCTTTCT
    AGAGAGTCTG GACTTGACAG AATTGATGAC CAACCTGGTT CATCGTCCAG TCACCATAGC
    CATGACAAGA TGGCGAGTAA AAGGATGGAC AGTGCAGACT CAGCTGTATC TCATGATAGC
    ATATCAAGTG AGCCAACTCA GTGCACCATG GTCTACTCAC GGCAGGGCAC AACAGAAACT
    ATTGAGGAAG TGGAAGATGA AGAGGAGGAA GAAAGCAAAA GTCCTTTATC AAAGATAGAA
    GCCGAGGAAG AAGAATATGA CATGGATTTG GAAGGGACAA GCTATACTCC GGACACAGAA
    TTTCCCCTGT ACGCCACTAC AGAGGGGGAT CTTCCTCCTT CGTACAGTAA AGCCGGCAGC
    TTCGAACAAC TTACATTTGT ATCTCAGGAC TCGGCTGACA ATTTAATGGT TCTAAGCCCA
    GATGAAAGTC GTACTGATAA GCTACATGAT CATATTCTTC CACCTCTTAC ACATGAACTG
    ACTGCTAGCG AGCTGCTTCT AAACAAAATG TTTTATGATG AAGAGTTAGA AGACTCTGAA
    AAATTTTATA CTACTCAACC ACGCCCGCTG CAGCTTATTT ATGCCTTGTA TAATACATTG
    GTGGCCAGAT CAGAAATGGT GTGCTATTTT GTGATTATTC TTAATCACAT GATTTCAGCT
    TCTATGATCA CATTGGTACT GCCCATCCTT ATATTCCTTT GGGCTATGCT TTCTGTCCCA
    AGGCCCAGTA AGAGATTTTG GATGACAGCT ATTGTATATA CAGAGGTGAC AATTGTTATT
    AAATATTTTT TCCAATTTGG ATTCTTCCCA TGGAATAAAA ATGTGGATTA TACAAAGGAC
    AAACCTTACT TCCCACCTAA CATAATGGGC ATTGAGAAGA AAGAAGGTTA TGTGCACTAT
    GACCTGGTGC AACTGCTTGC TCTGTTTTTC CATAGATCTA TTTTAAAGTG TCATGGGCTT
    TGGGATGAAG ATGATGTCAC TGAAAAAGCC ACAGATAAAG ACGACACGGA TGGAGAGATA
    TCAGAGGCAT CAGGAAGAAG GGATTCCGTT GGCTCATTAA AATCTGTCAA TCTAGCTGCG
    TCTGTGGAGT CAGTCCATGT GCACTTCCCT GAGGAAAAGA CCGCTATCAG GAGAAAAAGC
    ACAAGCAGTG GATCGCAAAT TTCCCGAAGG TCAAGTTATT CCTCGCACAG GTCTAAAAGA
    GGCAGCACCA GTACTCGGAA CAGCAGTCAA AAAGGAAGCA GCATTCCCAG TATTAAACTG
    AAATCTAGAA AGGAACTTAT ACTGGAAAAA CTCAGAGAAC AGCTCATTAA AGCAAAAGCA
    TTTACGATCA AGACGATACT ACAAATCTAT GTCCCGATCA GACAGTTTTT TTACAATCTT
    ATACATCCTG AATACAGTGC TGTGACTGAT GTCTATGTGC TGATGTTCCT GGCAGACACT
    GTAGATTTCA TCATTATTGT TTTTGGATTT TGGGCGTTTG GAAAACACTC CGCTGCAGCA
    GATATTACAT CCTCTCTATC AGAAGACCAA GTCCCAGAAG CATTTTTGGT AATGGTGCTT
    ATCCAGTTTG GAACCATGGT TGTTGATCGT GCTTTGTATC TTCGCAAAAC AGTCATGGGA
    AAAGTTATTT TCCAAGTTAT CCTTGTATTT GGAATCCATT TTTGGATGTT CTTCATCTTG
    CCAGGGGTAA CAGAAAGGAA ATTTAGTCAG AACACTGTTG CCCAGCTGTG GTATTTTGTC
    AAGTGCATTT ATTTTGGATT GTCGGCATAT CAGATACGTT GTGGATATCC AACTCGGGTG
    CTTGGTAACT TCCTTACTAA AAGCTACAAT TATGTCAACC TGTTCCTTTT CCAAGGCTTT
    CGCCTCGTTC CATTTCTTAC GGAGCTGCGA GCTGTTATGG ACTGGGTATG GACTGACACC
    ACTCTCAGTC TTTCCAGCTG GATCTGTGTA GAGGACATTT ATGCACATAT ATTTATACTA
    AAATGCTGGC GGGAGTCAGA AAAGAGATAT CCCCAGCCAC GAGGCCAAAA GAAGAAAAAG
    GTTGTGAAAT ACGGAATGGG TGGAATGATA ATAGTTCTGC TGATTTGTAT CGTGTGGTTT
    CCTTTGCTCT TCATGTCCTT GATTAAATCA GTTGCTGGGG TCACTAACAA GCCCCTAGAT
    GTGTCCATTA CAATAACCCT TGGAGGTTAT CAGCCTATTT TTACCATGAG TGCACAACAA
    AATCAGCTGC AAGGCTTGAA TGACAATGAA TTTAAGCATT TACATAATAT ATATAAAGGC
    AATACACCTG CCATGCAGTT TCTGGAATCC TATATGCAGG AAGACATCAC CATAGCAGGA
    CTGGAAGGCA ATTCCAACTC TCTGTGGACC ATCAGCCCTC CGAGCAGGAC AATGATGATA
    GAAAGACTTA TCCAGGAACC TGACTTCACA GCGGTCATTT CTTGGTCCAT TCGAAGAAAT
    GTGAGTCTTG GAGCTAAGGC AGAGGTAACA GGTGAAAAAT ATACAATATC ACTGGCAGGT
    CAAACTCGGA GGGAACTTGC GGATATGATG AACAGTTCAT CTCCCAATAA GACAGTGGAA
    CTGATAAAGA TATACCCATT GTGTTTAAAA GCACCAAGTG ATGTTACTGC AAAACCCATC
    CTTCAGCTTC TTAAGAATCA AGAGTTTGTG AATATTAGCG TTTTACTAAG AAAAGAAAAC
    ACATCAGAAG GGGTTCGAGA ATGGTGGGTA GTGAATCAAA GCCATAAAGA GTTCATTAAG
    AAGGATGGCC TGGAACTATG GGTTTTCAGT GACAAAGTCA GTCCTCCAAG TCTTGGATTC
    TTGGCAGGCT ATGGAATAAT GGGACTATAT GCCTCTGTAG TTCTCGTAAT AGGAAAGTTT
    GTGCGTGAGT TTTTCAGCGG AATTTCGCAC TCCATCATGT TTGAGGAGCT TCCCAACGTT
    GACCGTATAC TGAAGCTGTG CACAGACATT TTCCTTGTGA GAGAGACTGG AGAGTTGGAA
    CTGGAGGAAG ACCTGTATGC CAAATTAATA TTTTTGTACC GCTCCCCAGA AACTATGATC
    AAATGGACAA GAGAGAAAGA TAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa35457
    Clone ID Related Accession (Same CDS sequence) XM_012965363.2
    Accession Version XM_012965363.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8208bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product piezo-type mechanosensitive ion channel component 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012965363.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    ATGGCATCGG AAGTGGTGTG TGGGCTCATC TTCAGATTTC TTCTCCCACT ATGCCTGGTT 
    GTAGCATGCA TGTTCCGGTA CAATGGAATA TCTTTTGTCT ACCTCATCTA CCTCTTGCTT
    ATACCTTTAT TTTCGGAGCC TACCAAAATA ACAATGCAAG GTCATACAGG ACGGTTATTA
    AAATCCTTAT GTGTTTCCAG TCTGTCATTT CTGCTGCTGC ACATCATCTT TCAAATTACC
    GTATACAGCC TCGAGGCCAG AAAACACATT GAACCACATT TTAACTGTTC AACATGGGAG
    AAAGCAATAC GACAAATTGG TTTTGAAAGT GTAAAAGGAG CAGATGCTGG TAATGGAATC
    CGCGTGTTTG CACCAGATAT CGGAATGTTT ATTGCAAGCT TGGCTACCCT GTTGGTTTGT
    AGAAAACTAG TTCAGAAAAC AGTTACTGAA GAGGACGCAC AATACAACCC CCAGTTTGTA
    AATGAAGAAA TGGCAGAAGA AGGAAAGGTG GACCTGGATG AAGCGTTAAT GTATGAGGAA
    GACTTTGATG GAGAAGAGGA TGAAATTGAG GCAGCTGAAG AAAGCACAAA ATTAAAAATT
    CTTCGCAGAA TTGCTTCATT TGCATCCGTA CTTAAAGAGT TTATTGGCAA CATTATAACC
    ACCGCTGGGA AAGTAGTTAT TACAATTTTA CTTGGTTTAA CAGGCATGAT GTTGCCGTCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT TTGGGGCTAT GTACATGGTG GTCCTGTTGC
    CGAGTATTTG ATCCGCTGAT ATTCAGCTGT CTATGTGTAT TAATGGCTAT ATTTAGTGCA
    GGGCACTTAA TTGGACTTTA TCTGTACCAG TTGCAGTTTT TTCACGAGAT TATTCCACCA
    AATGATTTCT ATGCCAGGTT GTTTGGTGTT ACTTCATTAG TTCAAACCGA CTGCTGTAAC
    ACTTGGAGAT TTTTTGCAAA CAACAGTCTA CCATGGAACC AGTATACCAA TCCTGTCATG
    CTTTTAGTAC TGTATTACAC GCTGGCAACT CTCATACGCC TTTGGCTACA GGAACCTATT
    AATGTAGAGG AAGAGAAGGA GGAAGACTGT AGCCCAGCAG CACTTACTGC AGAAAGAAGA
    CGTAGTTTGT GGTACTCTAG CCACTACACA ACGGATGAAA GACAACTTCT GTCCATGACT
    CAAGATGACT ACAAGCCATC TGATGTTTTA CTAGTAACTG TAAATGGGAC TCCCGTAGAT
    TACTGTTCAC TAAATTCATC ACTTCCGATA GAAAATGGTC CAACTAAAAT TGACATGTAT
    TCAACACCGC AATATAAATG GGACCATGAA GATGAAACTT CAGAAAAGAA AGAAGAGGAA
    GAGCAAGAAG AAGAACCCAA TGAAGAGGTT GCAGAAGAGA AAGAAAACAC TAAAGTTCAT
    GTAATGGTGG TGGCATTTCA GTTCATTATG AAGCAAAGCT ATATATGTGC ACTAATTTCA
    ATGATGGCCT GGAGCATCAC ATACCACAGC TGGTTGACTT TTGTTTTGTT AATCTGGTCT
    TGTGCTCTCT GGATGGTCCG AAATAGGAGA AAATATGCTA TGCTTAGTTC ACCTTTTATG
    GTTATGTATG GTAATTTACT TTTAACATTA CAATACATAT GGAGCATAGC CTTACAAAGT
    GATGAACTTC CAGAAGTTAT GGGTCTCCTT GAGAGGAAAA AGCCCGAAGA GCTTGTATCG
    AAGATCATTT TCACAGTTAC ATTTTGGCTT TTGCTCAGAC AACACCTTAC AGAGCAAAAG
    GCATTAAAGC AAAAAGAATT AGCATTATCT GAAATAAAAG TTGGAAGTGA AGACAAAGAA
    GGTGAAGTGC AAGAAGAAGA AGAAGAAGTG GAGGATGAAA ATGATATCAT GAAGGTCCTC
    GGAAAGCTAG TGCAGGCTAT GTTTGTCAAG TACTGGATTT ATGTCTGTGG CGGCATGTTT
    TTTTTTGTCA GCTTTGAAGG AAAAATAGTT ATGTACAAAA TTATCTACAT GATGCTCTTC
    CTATTCTGTG TAGCTCTGTA TCAGGTGCAC TATGAATGGT GGAGACGAAT TTTAAAATAT
    TTTTGGATGT CTGTGGTTGT TTACACCATG TTGGTGCTTG TTTTTATTTA CACTTACCAA
    TTTAAATCAT TTCCTGGCCT TTGGATGAAT ATGACAGGGC TGGATAAGGA AAAGTTAGCA
    GATCTTGGTT TAGAGACATT TAACTTGACT GACCTATTTA CTCGTATATT TATCCCAACC
    TCATTTTTGT TAGTGTGCAT TTTGCACCTA CATTACTTCC ATGACCGTTT TCTCCAGCTC
    ACTGACCTAA AGGCTGTAAC CAGCAAGCAG GACAGCACAA TTTACAGCCA TGCCAAAGTA
    AATGGTCGAG TTTACTTAAT AATAAATAGC ATCAAGCAAA AAATTCCAAT TCAGCAAAAT
    GAATTGGTGC ATCCAGAAGG AAGTTTGCCT GATATAACGA TGATGAACTT AACAACAGAA
    TCTGAAATGC TTGAGGAAAA GCGGTTGGAA ATTTATGACG AAAAAAAAAC TCATGACCAA
    GACTCAGATG ATGAATGGGA TAAAGGGGAT AGCAAAGAAC CAGGTCATTC TGATGATGAA
    GCTGAGGATG AGGAATCAGA AGAAGAAGAA ACAACAACAG AGTCCAGGAA CAAGTGGCAT
    CTAGTGATAG ATCGCTTAAC GGTTCTTTTC CTAAAGTTTC TGGAATATTT TCACAAGTTT
    CAGGTCTTTG TTTGGTGGCT TCTGGAGCTT CACATGATAA AGATTGTTTC TTCATACATC
    ATTTGGCTTA CTATAAAGGA GGTTTCACTG CTCAATTATG TATTTTTTAT TGCCTGGGCC
    TTCGCTTTGC CCTACTCACA ATTCCGGCCA TTGGCATCAA GTGTTTGCAC AGTCTGGACA
    TGTGTCATCA TCATATGTAA AATGTTGTAC CAGCTAGCCT CCATTGACCC TGCAACCTTT
    TCCAGCAACT GTTTAATGCC ACAGCTAAAC GAAACAACTG CACGTAACCA AACTGAGCTT
    AATTCTTCTG TGCTTTACAA TTCCTCTGTT GATCCTTCTA ATTGGGTTGG ACTAAAGAAG
    TCAACTCCAC TCCTTGCTTA TTTGCGGAAC AATTTGCTGA TGTTGGCCAT GTTAGCTTTT
    GAAGTAACCA TTTACCGGCA TCAGGAGTAT TACAGGTTGC GAAACAATCT CACCACACCT
    GCAACAAAAA CCATCTTTCA TGACATCACA AGACTTCACT TGGATGATGG ACTCATAAAT
    AGTGCCAAGT ATTTTGTGAA CTACTTTTTC TACAAGTTTG GATTGGAAAC ATGTTTCTTA
    CTGTCGGTAA ATGTAATCGG TCAGAGGATG GACTTTTTTG CCATGGTTCA TGCACTTTGG
    CTTTTTGGCG TATTATACCG ACGCAGAAGG AAAGCGATTG CAGAAACCTG GCACAGATAT
    TGCTGTTTTC TTGCTTGCAT TATGGCATTC CAGTATTTTA TATGTATTGG CATCCCCCCT
    GCTGCATGTA AAGACTATCC TTGGAGACAA CCTGGAGCCA ATTTTAATTC TAACATAATA
    AAGTGGTTAT ACTTTCCAGA CTTTATTGTG AGGCCTAGTC CTCTGTTTCT TGTCTATGAT
    TTTTTGCTTT TGCTTTGTGC GTCCCTTCAA AGAGAAGTGT TTGAGGAAGA AAATAAGGCA
    GCAGTTCGAA TCATGGCTGG TGACAATGTA GAAATTTGCA TGAACCTTGA TGCTGCATCT
    TTTAGCCAAC ATAATCCTGT ACCTGATTTC ATATACTGCA GATCTTATTT GGACATGGGT
    AAAGTTATAA TGTTTAGTTA CCTTTTCTGG TTTGTGCTCA CCATCATCTT CATCACGGGA
    ACTACACGGA TCAGCATATT CTGCATGGGC TACTTGGTAG CTTGCTTCTA TTTTCTATTA
    TTTGGTGGAG ATTTGCTGTT AAAACCTATA AGAATCATCC TACGCTATTG GGATTGGCTG
    ATTGCTTACA ATGTTTTTGT CATTACAATG AAAAATGTAT TATCAATAGG AGCATGTGGC
    TACATTGAAT ATTTAATCAA AGACAGTTGT TGGTTAATTC AGGCATTCAG TCTTTCTTGT
    ACAGTGAAAG GTTATTTTAT CCCCCAAAAC ACACCTGACT GTCAGTTGCC AAGCGGAGAG
    GCTGGAATCA TTTGGGACAG TATATGTTTT GCTTTCCTTT TATTGCAAAG AAGAGTGTTT
    ATGAGCTATT ACTTCTTACA TGTGGTTGCA GACATCAAAT CATCTCAGAT TCTAGCATCA
    AGAGGTGCTG AACTGTTCCA AGCTACAATT GTAAAAGCTG TAAAAGCAAG AATTGAAGAA
    GAAAAAAAAT CTATGGACCA GCTCAAGAGA CAGATGGACC ACATTAAAGC CAGGCAAGAA
    AAATATAAAA AAGGTAAAGA AAAAATGCTG AGTCTTACAC AGGATGGAGA AGGACAAGAA
    GTTCAAAAAC TAGATGATGA CGATGAAGAA GGAGACAAAG CAGACAAAGA GAAAGCCAAG
    GGCAAAAAAA AGCAGTGGTG GCGACCTTGG GTTGATCATG CTTCCATGGT GAAAAGCGGT
    GATTATTATT TGTTTGAGAC GGACAGTGAA GAAGAAGATG AGGATGACAA AAAAGAAGAA
    GAGCCTAGAA GAAAATCTGC CTTCCAGTTT TTGTACCAAG CATGGATTAC TGATCCAAAA
    ACAGCCCTTA AGCAAAGAAG CATGGAAGCC AAAGAAGAAA AGGCAAGGGA AATGAGGCGA
    AAGGGCATTG CTGAAGGTGA TATTATTGTA GAATGTGGGG ATAAAGAAGA AGAAAAAGAG
    CAAGGAAAAA AGAAATCAGA TGGCCCAGAC AATATCTTCA AAAGAATATT TAACATCCTG
    AAGTTTACTT GGGTCCTCTT CCTTGCTACA ATCGATAGTT TTACGGCTTG GCTAAACTCC
    ATTTCAAGAG AACATATTGA TATCTCCAAA GTTCTAAGCA TAGAACGTTG CATGCTGACT
    AGAGAGATAA AAAAGGGCAA TGTGCCTACT CGAGAAAGCA TCCACATGTA TTACCAAAAT
    CAAATAATGA TGAGCCTTTC TAGAGAGTCT GGACTTGACA GAATTGATGA CCAACCTGGT
    TCATCGTCCA GTCACCATAG CCATGACAAG ATGGCGAGTA AAAGGATGGA CAGTGCAGAC
    TCAGCTGTAT CTCATGATAG CATATCAAGT GAGCCAACTC AGTGCACCAT GGTCTACTCA
    CGGCAGGGCA CAACAGAAAC TATTGAGGAA GTGGAAGATG AAGAGGAGGA AGAAAGCAAA
    AGTCCTTTAT CAAAGATAGA AGCCGAGGAA GAAGAATATG ACATGGATTT GGAAGGGACA
    AGCTATACTC CGGACACAGA ATTTCCCCTG TACGCCACTA CAGAGGGGGA TCTTCCTCCT
    TCGTACAGTA AAGCCGGCAG CTTCGAACAA CTTACATTTG TATCTCAGGA CTCGGCTGAC
    AATTTAATGG TTCTAAGCCC AGATGAAAGT CGTACTGATA AGCTACATGA TCATATTCTT
    CCACCTCTTA CACATGAACT GACTGCTAGC GAGCTGCTTC TAAACAAAAT GTTTTATGAT
    GAAGAGTTAG AAGACTCTGA AAAATTTTAT ACTACTCAAC CACGCCCGCT GCAGCTTATT
    TATGCCTTGT ATAATACATT GGTGGCCAGA TCAGAAATGG TGTGCTATTT TGTGATTATT
    CTTAATCACA TGATTTCAGC TTCTATGATC ACATTGGTAC TGCCCATCCT TATATTCCTT
    TGGGCTATGC TTTCTGTCCC AAGGCCCAGT AAGAGATTTT GGATGACAGC TATTGTATAT
    ACAGAGGTGA CAATTGTTAT TAAATATTTT TTCCAATTTG GATTCTTCCC ATGGAATAAA
    AATGTGGATT ATACAAAGGA CAAACCTTAC TTCCCACCTA ACATAATGGG CATTGAGAAG
    AAAGAAGGTT ATGTGCACTA TGACCTGGTG CAACTGCTTG CTCTGTTTTT CCATAGATCT
    ATTTTAAAGT GTCATGGGCT TTGGGATGAA GATGATGTCA CTGAAAAAGC CACAGATAAA
    GACGACACGG ATGGAGAGAT ATCAGAGGCA TCAGGAAGAA GGGATTCCGT TGGCTCATTA
    AAATCTGTCA ATCTAGCTGC GTCTGTGGAG TCAGTCCATG TGCACTTCCC TGAGGAAAAG
    ACCGCTATCA GGAGAAAAAG CACAAGCAGT GGATCGCAAA TTTCCCGAAG GTCAAGTTAT
    TCCTCGCACA GGTCTAAAAG AGGCAGCACC AGTACTCGGA ACAGCAGTCA AAAAGGAAGC
    AGCATTCCCA GTATTAAACT GAAATCTAGA AAGGAACTTA TACTGGAAAA ACTCAGAGAA
    CAGCTCATTA AAGCAAAAGC ATTTACGATC AAGACGATAC TACAAATCTA TGTCCCGATC
    AGACAGTTTT TTTACAATCT TATACATCCT GAATACAGTG CTGTGACTGA TGTCTATGTG
    CTGATGTTCC TGGCAGACAC TGTAGATTTC ATCATTATTG TTTTTGGATT TTGGGCGTTT
    GGAAAACACT CCGCTGCAGC AGATATTACA TCCTCTCTAT CAGAAGACCA AGTCCCAGAA
    GCATTTTTGG TAATGGTGCT TATCCAGTTT GGAACCATGG TTGTTGATCG TGCTTTGTAT
    CTTCGCAAAA CAGTCATGGG AAAAGTTATT TTCCAAGTTA TCCTTGTATT TGGAATCCAT
    TTTTGGATGT TCTTCATCTT GCCAGGGGTA ACAGAAAGGA AATTTAGTCA GAACACTGTT
    GCCCAGCTGT GGTATTTTGT CAAGTGCATT TATTTTGGAT TGTCGGCATA TCAGATACGT
    TGTGGATATC CAACTCGGGT GCTTGGTAAC TTCCTTACTA AAAGCTACAA TTATGTCAAC
    CTGTTCCTTT TCCAAGGCTT TCGCCTCGTT CCATTTCTTA CGGAGCTGCG AGCTGTTATG
    GACTGGGTAT GGACTGACAC CACTCTCAGT CTTTCCAGCT GGATCTGTGT AGAGGACATT
    TATGCACATA TATTTATACT AAAATGCTGG CGGGAGTCAG AAAAGAGATA TCCCCAGCCA
    CGAGGCCAAA AGAAGAAAAA GGTTGTGAAA TACGGAATGG GTGGAATGAT AATAGTTCTG
    CTGATTTGTA TCGTGTGGTT TCCTTTGCTC TTCATGTCCT TGATTAAATC AGTTGCTGGG
    GTCACTAACA AGCCCCTAGA TGTGTCCATT ACAATAACCC TTGGAGGTTA TCAGCCTATT
    TTTACCATGA GTGCACAACA AAATCAGCTG CAAGGCTTGA ATGACAATGA ATTTAAGCAT
    TTACATAATA TATATAAAGG CAATACACCT GCCATGCAGT TTCTGGAATC CTATATGCAG
    GAAGACATCA CCATAGCAGG ACTGGAAGGC AATTCCAACT CTCTGTGGAC CATCAGCCCT
    CCGAGCAGGA CAATGATGAT AGAAAGACTT ATCCAGGAAC CTGACTTCAC AGCGGTCATT
    TCTTGGTCCA TTCGAAGAAA TGTGAGTCTT GGAGCTAAGG CAGAGGTAAC AGGTGAAAAA
    TATACAATAT CACTGGCAGG TCAAACTCGG AGGGAACTTG CGGATATGAT GAACAGTTCA
    TCTCCCAATA AGACAGTGGA ACTGATAAAG ATATACCCAT TGTGTTTAAA AGCACCAAGT
    GATGTTACTG CAAAACCCAT CCTTCAGCTT CTTAAGAATC AAGAGTTTGT GAATATTAGC
    GTTTTACTAA GAAAAGAAAA CACATCAGAA GGGGTTCGAG AATGGTGGGT AGTGAATCAA
    AGCCATAAAG AGTTCATTAA GAAGGATGGC CTGGAACTAT GGGTTTTCAG TGACAAAGTC
    AGTCCTCCAA GTCTTGGATT CTTGGCAGGC TATGGAATAA TGGGACTATA TGCCTCTGTA
    GTTCTCGTAA TAGGAAAGTT TGTGCGTGAG TTTTTCAGCG GAATTTCGCA CTCCATCATG
    TTTGAGGAGC TTCCCAACGT TGACCGTATA CTGAAGCTGT GCACAGACAT TTTCCTTGTG
    AGAGAGACTG GAGAGTTGGA ACTGGAGGAA GACCTGTATG CCAAATTAAT ATTTTTGTAC
    CGCTCCCCAG AAACTATGAT CAAATGGACA AGAGAGAAAG ATAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012820817.1
    CDS612..8819
    Translation

    Target ORF information:

    RefSeq Version XM_012965363.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis piezo-type mechanosensitive ion channel component 2 (piezo2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012965363.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    ATGGCATCGG AAGTGGTGTG TGGGCTCATC TTCAGATTTC TTCTCCCACT ATGCCTGGTT 
    GTAGCATGCA TGTTCCGGTA CAATGGAATA TCTTTTGTCT ACCTCATCTA CCTCTTGCTT
    ATACCTTTAT TTTCGGAGCC TACCAAAATA ACAATGCAAG GTCATACAGG ACGGTTATTA
    AAATCCTTAT GTGTTTCCAG TCTGTCATTT CTGCTGCTGC ACATCATCTT TCAAATTACC
    GTATACAGCC TCGAGGCCAG AAAACACATT GAACCACATT TTAACTGTTC AACATGGGAG
    AAAGCAATAC GACAAATTGG TTTTGAAAGT GTAAAAGGAG CAGATGCTGG TAATGGAATC
    CGCGTGTTTG CACCAGATAT CGGAATGTTT ATTGCAAGCT TGGCTACCCT GTTGGTTTGT
    AGAAAACTAG TTCAGAAAAC AGTTACTGAA GAGGACGCAC AATACAACCC CCAGTTTGTA
    AATGAAGAAA TGGCAGAAGA AGGAAAGGTG GACCTGGATG AAGCGTTAAT GTATGAGGAA
    GACTTTGATG GAGAAGAGGA TGAAATTGAG GCAGCTGAAG AAAGCACAAA ATTAAAAATT
    CTTCGCAGAA TTGCTTCATT TGCATCCGTA CTTAAAGAGT TTATTGGCAA CATTATAACC
    ACCGCTGGGA AAGTAGTTAT TACAATTTTA CTTGGTTTAA CAGGCATGAT GTTGCCGTCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT TTGGGGCTAT GTACATGGTG GTCCTGTTGC
    CGAGTATTTG ATCCGCTGAT ATTCAGCTGT CTATGTGTAT TAATGGCTAT ATTTAGTGCA
    GGGCACTTAA TTGGACTTTA TCTGTACCAG TTGCAGTTTT TTCACGAGAT TATTCCACCA
    AATGATTTCT ATGCCAGGTT GTTTGGTGTT ACTTCATTAG TTCAAACCGA CTGCTGTAAC
    ACTTGGAGAT TTTTTGCAAA CAACAGTCTA CCATGGAACC AGTATACCAA TCCTGTCATG
    CTTTTAGTAC TGTATTACAC GCTGGCAACT CTCATACGCC TTTGGCTACA GGAACCTATT
    AATGTAGAGG AAGAGAAGGA GGAAGACTGT AGCCCAGCAG CACTTACTGC AGAAAGAAGA
    CGTAGTTTGT GGTACTCTAG CCACTACACA ACGGATGAAA GACAACTTCT GTCCATGACT
    CAAGATGACT ACAAGCCATC TGATGTTTTA CTAGTAACTG TAAATGGGAC TCCCGTAGAT
    TACTGTTCAC TAAATTCATC ACTTCCGATA GAAAATGGTC CAACTAAAAT TGACATGTAT
    TCAACACCGC AATATAAATG GGACCATGAA GATGAAACTT CAGAAAAGAA AGAAGAGGAA
    GAGCAAGAAG AAGAACCCAA TGAAGAGGTT GCAGAAGAGA AAGAAAACAC TAAAGTTCAT
    GTAATGGTGG TGGCATTTCA GTTCATTATG AAGCAAAGCT ATATATGTGC ACTAATTTCA
    ATGATGGCCT GGAGCATCAC ATACCACAGC TGGTTGACTT TTGTTTTGTT AATCTGGTCT
    TGTGCTCTCT GGATGGTCCG AAATAGGAGA AAATATGCTA TGCTTAGTTC ACCTTTTATG
    GTTATGTATG GTAATTTACT TTTAACATTA CAATACATAT GGAGCATAGC CTTACAAAGT
    GATGAACTTC CAGAAGTTAT GGGTCTCCTT GAGAGGAAAA AGCCCGAAGA GCTTGTATCG
    AAGATCATTT TCACAGTTAC ATTTTGGCTT TTGCTCAGAC AACACCTTAC AGAGCAAAAG
    GCATTAAAGC AAAAAGAATT AGCATTATCT GAAATAAAAG TTGGAAGTGA AGACAAAGAA
    GGTGAAGTGC AAGAAGAAGA AGAAGAAGTG GAGGATGAAA ATGATATCAT GAAGGTCCTC
    GGAAAGCTAG TGCAGGCTAT GTTTGTCAAG TACTGGATTT ATGTCTGTGG CGGCATGTTT
    TTTTTTGTCA GCTTTGAAGG AAAAATAGTT ATGTACAAAA TTATCTACAT GATGCTCTTC
    CTATTCTGTG TAGCTCTGTA TCAGGTGCAC TATGAATGGT GGAGACGAAT TTTAAAATAT
    TTTTGGATGT CTGTGGTTGT TTACACCATG TTGGTGCTTG TTTTTATTTA CACTTACCAA
    TTTAAATCAT TTCCTGGCCT TTGGATGAAT ATGACAGGGC TGGATAAGGA AAAGTTAGCA
    GATCTTGGTT TAGAGACATT TAACTTGACT GACCTATTTA CTCGTATATT TATCCCAACC
    TCATTTTTGT TAGTGTGCAT TTTGCACCTA CATTACTTCC ATGACCGTTT TCTCCAGCTC
    ACTGACCTAA AGGCTGTAAC CAGCAAGCAG GACAGCACAA TTTACAGCCA TGCCAAAGTA
    AATGGTCGAG TTTACTTAAT AATAAATAGC ATCAAGCAAA AAATTCCAAT TCAGCAAAAT
    GAATTGGTGC ATCCAGAAGG AAGTTTGCCT GATATAACGA TGATGAACTT AACAACAGAA
    TCTGAAATGC TTGAGGAAAA GCGGTTGGAA ATTTATGACG AAAAAAAAAC TCATGACCAA
    GACTCAGATG ATGAATGGGA TAAAGGGGAT AGCAAAGAAC CAGGTCATTC TGATGATGAA
    GCTGAGGATG AGGAATCAGA AGAAGAAGAA ACAACAACAG AGTCCAGGAA CAAGTGGCAT
    CTAGTGATAG ATCGCTTAAC GGTTCTTTTC CTAAAGTTTC TGGAATATTT TCACAAGTTT
    CAGGTCTTTG TTTGGTGGCT TCTGGAGCTT CACATGATAA AGATTGTTTC TTCATACATC
    ATTTGGCTTA CTATAAAGGA GGTTTCACTG CTCAATTATG TATTTTTTAT TGCCTGGGCC
    TTCGCTTTGC CCTACTCACA ATTCCGGCCA TTGGCATCAA GTGTTTGCAC AGTCTGGACA
    TGTGTCATCA TCATATGTAA AATGTTGTAC CAGCTAGCCT CCATTGACCC TGCAACCTTT
    TCCAGCAACT GTTTAATGCC ACAGCTAAAC GAAACAACTG CACGTAACCA AACTGAGCTT
    AATTCTTCTG TGCTTTACAA TTCCTCTGTT GATCCTTCTA ATTGGGTTGG ACTAAAGAAG
    TCAACTCCAC TCCTTGCTTA TTTGCGGAAC AATTTGCTGA TGTTGGCCAT GTTAGCTTTT
    GAAGTAACCA TTTACCGGCA TCAGGAGTAT TACAGGTTGC GAAACAATCT CACCACACCT
    GCAACAAAAA CCATCTTTCA TGACATCACA AGACTTCACT TGGATGATGG ACTCATAAAT
    AGTGCCAAGT ATTTTGTGAA CTACTTTTTC TACAAGTTTG GATTGGAAAC ATGTTTCTTA
    CTGTCGGTAA ATGTAATCGG TCAGAGGATG GACTTTTTTG CCATGGTTCA TGCACTTTGG
    CTTTTTGGCG TATTATACCG ACGCAGAAGG AAAGCGATTG CAGAAACCTG GCACAGATAT
    TGCTGTTTTC TTGCTTGCAT TATGGCATTC CAGTATTTTA TATGTATTGG CATCCCCCCT
    GCTGCATGTA AAGACTATCC TTGGAGACAA CCTGGAGCCA ATTTTAATTC TAACATAATA
    AAGTGGTTAT ACTTTCCAGA CTTTATTGTG AGGCCTAGTC CTCTGTTTCT TGTCTATGAT
    TTTTTGCTTT TGCTTTGTGC GTCCCTTCAA AGAGAAGTGT TTGAGGAAGA AAATAAGGCA
    GCAGTTCGAA TCATGGCTGG TGACAATGTA GAAATTTGCA TGAACCTTGA TGCTGCATCT
    TTTAGCCAAC ATAATCCTGT ACCTGATTTC ATATACTGCA GATCTTATTT GGACATGGGT
    AAAGTTATAA TGTTTAGTTA CCTTTTCTGG TTTGTGCTCA CCATCATCTT CATCACGGGA
    ACTACACGGA TCAGCATATT CTGCATGGGC TACTTGGTAG CTTGCTTCTA TTTTCTATTA
    TTTGGTGGAG ATTTGCTGTT AAAACCTATA AGAATCATCC TACGCTATTG GGATTGGCTG
    ATTGCTTACA ATGTTTTTGT CATTACAATG AAAAATGTAT TATCAATAGG AGCATGTGGC
    TACATTGAAT ATTTAATCAA AGACAGTTGT TGGTTAATTC AGGCATTCAG TCTTTCTTGT
    ACAGTGAAAG GTTATTTTAT CCCCCAAAAC ACACCTGACT GTCAGTTGCC AAGCGGAGAG
    GCTGGAATCA TTTGGGACAG TATATGTTTT GCTTTCCTTT TATTGCAAAG AAGAGTGTTT
    ATGAGCTATT ACTTCTTACA TGTGGTTGCA GACATCAAAT CATCTCAGAT TCTAGCATCA
    AGAGGTGCTG AACTGTTCCA AGCTACAATT GTAAAAGCTG TAAAAGCAAG AATTGAAGAA
    GAAAAAAAAT CTATGGACCA GCTCAAGAGA CAGATGGACC ACATTAAAGC CAGGCAAGAA
    AAATATAAAA AAGGTAAAGA AAAAATGCTG AGTCTTACAC AGGATGGAGA AGGACAAGAA
    GTTCAAAAAC TAGATGATGA CGATGAAGAA GGAGACAAAG CAGACAAAGA GAAAGCCAAG
    GGCAAAAAAA AGCAGTGGTG GCGACCTTGG GTTGATCATG CTTCCATGGT GAAAAGCGGT
    GATTATTATT TGTTTGAGAC GGACAGTGAA GAAGAAGATG AGGATGACAA AAAAGAAGAA
    GAGCCTAGAA GAAAATCTGC CTTCCAGTTT TTGTACCAAG CATGGATTAC TGATCCAAAA
    ACAGCCCTTA AGCAAAGAAG CATGGAAGCC AAAGAAGAAA AGGCAAGGGA AATGAGGCGA
    AAGGGCATTG CTGAAGGTGA TATTATTGTA GAATGTGGGG ATAAAGAAGA AGAAAAAGAG
    CAAGGAAAAA AGAAATCAGA TGGCCCAGAC AATATCTTCA AAAGAATATT TAACATCCTG
    AAGTTTACTT GGGTCCTCTT CCTTGCTACA ATCGATAGTT TTACGGCTTG GCTAAACTCC
    ATTTCAAGAG AACATATTGA TATCTCCAAA GTTCTAAGCA TAGAACGTTG CATGCTGACT
    AGAGAGATAA AAAAGGGCAA TGTGCCTACT CGAGAAAGCA TCCACATGTA TTACCAAAAT
    CAAATAATGA TGAGCCTTTC TAGAGAGTCT GGACTTGACA GAATTGATGA CCAACCTGGT
    TCATCGTCCA GTCACCATAG CCATGACAAG ATGGCGAGTA AAAGGATGGA CAGTGCAGAC
    TCAGCTGTAT CTCATGATAG CATATCAAGT GAGCCAACTC AGTGCACCAT GGTCTACTCA
    CGGCAGGGCA CAACAGAAAC TATTGAGGAA GTGGAAGATG AAGAGGAGGA AGAAAGCAAA
    AGTCCTTTAT CAAAGATAGA AGCCGAGGAA GAAGAATATG ACATGGATTT GGAAGGGACA
    AGCTATACTC CGGACACAGA ATTTCCCCTG TACGCCACTA CAGAGGGGGA TCTTCCTCCT
    TCGTACAGTA AAGCCGGCAG CTTCGAACAA CTTACATTTG TATCTCAGGA CTCGGCTGAC
    AATTTAATGG TTCTAAGCCC AGATGAAAGT CGTACTGATA AGCTACATGA TCATATTCTT
    CCACCTCTTA CACATGAACT GACTGCTAGC GAGCTGCTTC TAAACAAAAT GTTTTATGAT
    GAAGAGTTAG AAGACTCTGA AAAATTTTAT ACTACTCAAC CACGCCCGCT GCAGCTTATT
    TATGCCTTGT ATAATACATT GGTGGCCAGA TCAGAAATGG TGTGCTATTT TGTGATTATT
    CTTAATCACA TGATTTCAGC TTCTATGATC ACATTGGTAC TGCCCATCCT TATATTCCTT
    TGGGCTATGC TTTCTGTCCC AAGGCCCAGT AAGAGATTTT GGATGACAGC TATTGTATAT
    ACAGAGGTGA CAATTGTTAT TAAATATTTT TTCCAATTTG GATTCTTCCC ATGGAATAAA
    AATGTGGATT ATACAAAGGA CAAACCTTAC TTCCCACCTA ACATAATGGG CATTGAGAAG
    AAAGAAGGTT ATGTGCACTA TGACCTGGTG CAACTGCTTG CTCTGTTTTT CCATAGATCT
    ATTTTAAAGT GTCATGGGCT TTGGGATGAA GATGATGTCA CTGAAAAAGC CACAGATAAA
    GACGACACGG ATGGAGAGAT ATCAGAGGCA TCAGGAAGAA GGGATTCCGT TGGCTCATTA
    AAATCTGTCA ATCTAGCTGC GTCTGTGGAG TCAGTCCATG TGCACTTCCC TGAGGAAAAG
    ACCGCTATCA GGAGAAAAAG CACAAGCAGT GGATCGCAAA TTTCCCGAAG GTCAAGTTAT
    TCCTCGCACA GGTCTAAAAG AGGCAGCACC AGTACTCGGA ACAGCAGTCA AAAAGGAAGC
    AGCATTCCCA GTATTAAACT GAAATCTAGA AAGGAACTTA TACTGGAAAA ACTCAGAGAA
    CAGCTCATTA AAGCAAAAGC ATTTACGATC AAGACGATAC TACAAATCTA TGTCCCGATC
    AGACAGTTTT TTTACAATCT TATACATCCT GAATACAGTG CTGTGACTGA TGTCTATGTG
    CTGATGTTCC TGGCAGACAC TGTAGATTTC ATCATTATTG TTTTTGGATT TTGGGCGTTT
    GGAAAACACT CCGCTGCAGC AGATATTACA TCCTCTCTAT CAGAAGACCA AGTCCCAGAA
    GCATTTTTGG TAATGGTGCT TATCCAGTTT GGAACCATGG TTGTTGATCG TGCTTTGTAT
    CTTCGCAAAA CAGTCATGGG AAAAGTTATT TTCCAAGTTA TCCTTGTATT TGGAATCCAT
    TTTTGGATGT TCTTCATCTT GCCAGGGGTA ACAGAAAGGA AATTTAGTCA GAACACTGTT
    GCCCAGCTGT GGTATTTTGT CAAGTGCATT TATTTTGGAT TGTCGGCATA TCAGATACGT
    TGTGGATATC CAACTCGGGT GCTTGGTAAC TTCCTTACTA AAAGCTACAA TTATGTCAAC
    CTGTTCCTTT TCCAAGGCTT TCGCCTCGTT CCATTTCTTA CGGAGCTGCG AGCTGTTATG
    GACTGGGTAT GGACTGACAC CACTCTCAGT CTTTCCAGCT GGATCTGTGT AGAGGACATT
    TATGCACATA TATTTATACT AAAATGCTGG CGGGAGTCAG AAAAGAGATA TCCCCAGCCA
    CGAGGCCAAA AGAAGAAAAA GGTTGTGAAA TACGGAATGG GTGGAATGAT AATAGTTCTG
    CTGATTTGTA TCGTGTGGTT TCCTTTGCTC TTCATGTCCT TGATTAAATC AGTTGCTGGG
    GTCACTAACA AGCCCCTAGA TGTGTCCATT ACAATAACCC TTGGAGGTTA TCAGCCTATT
    TTTACCATGA GTGCACAACA AAATCAGCTG CAAGGCTTGA ATGACAATGA ATTTAAGCAT
    TTACATAATA TATATAAAGG CAATACACCT GCCATGCAGT TTCTGGAATC CTATATGCAG
    GAAGACATCA CCATAGCAGG ACTGGAAGGC AATTCCAACT CTCTGTGGAC CATCAGCCCT
    CCGAGCAGGA CAATGATGAT AGAAAGACTT ATCCAGGAAC CTGACTTCAC AGCGGTCATT
    TCTTGGTCCA TTCGAAGAAA TGTGAGTCTT GGAGCTAAGG CAGAGGTAAC AGGTGAAAAA
    TATACAATAT CACTGGCAGG TCAAACTCGG AGGGAACTTG CGGATATGAT GAACAGTTCA
    TCTCCCAATA AGACAGTGGA ACTGATAAAG ATATACCCAT TGTGTTTAAA AGCACCAAGT
    GATGTTACTG CAAAACCCAT CCTTCAGCTT CTTAAGAATC AAGAGTTTGT GAATATTAGC
    GTTTTACTAA GAAAAGAAAA CACATCAGAA GGGGTTCGAG AATGGTGGGT AGTGAATCAA
    AGCCATAAAG AGTTCATTAA GAAGGATGGC CTGGAACTAT GGGTTTTCAG TGACAAAGTC
    AGTCCTCCAA GTCTTGGATT CTTGGCAGGC TATGGAATAA TGGGACTATA TGCCTCTGTA
    GTTCTCGTAA TAGGAAAGTT TGTGCGTGAG TTTTTCAGCG GAATTTCGCA CTCCATCATG
    TTTGAGGAGC TTCCCAACGT TGACCGTATA CTGAAGCTGT GCACAGACAT TTTCCTTGTG
    AGAGAGACTG GAGAGTTGGA ACTGGAGGAA GACCTGTATG CCAAATTAAT ATTTTTGTAC
    CGCTCCCCAG AAACTATGAT CAAATGGACA AGAGAGAAAG ATAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa35459
    Clone ID Related Accession (Same CDS sequence) XM_012965365.2
    Accession Version XM_012965365.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8133bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product piezo-type mechanosensitive ion channel component 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012965365.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    ATGGCATCGG AAGTGGTGTG TGGGCTCATC TTCAGATTTC TTCTCCCACT ATGCCTGGTT 
    GTAGCATGCA TGTTCCGGTA CAATGGAATA TCTTTTGTCT ACCTCATCTA CCTCTTGCTT
    ATACCTTTAT TTTCGGAGCC TACCAAAATA ACAATGCAAG GTCATACAGG ACGGTTATTA
    AAATCCTTAT GTGTTTCCAG TCTGTCATTT CTGCTGCTGC ACATCATCTT TCAAATTACC
    GTATACAGCC TCGAGGCCAG AAAACACATT GAACCACATT TTAACTGTTC AACATGGGAG
    AAAGCAATAC GACAAATTGG TTTTGAAAGT GTAAAAGGAG CAGATGCTGG TAATGGAATC
    CGCGTGTTTG CACCAGATAT CGGAATGTTT ATTGCAAGCT TGGCTACCCT GTTGGTTTGT
    AGAAAACTAG TTCAGAAAAC AGTTACTGAA GAGGACGCAC AATACAACCC CCAGTTTGTA
    AATGAAGAAA TGGCAGAAGA AGGAAAGGTG GACCTGGATG AAGCGTTAAT GTATGAGGAA
    GACTTTGATG GAGAAGAGGA TGAAATTGAG GCAGCTGAAG AAAGCACAAA ATTAAAAATT
    CTTCGCAGAA TTGCTTCATT TGCATCCGTA CTTAAAGAGT TTATTGGCAA CATTATAACC
    ACCGCTGGGA AAGTAGTTAT TACAATTTTA CTTGGTTTAA CAGGCATGAT GTTGCCGTCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT TTGGGGCTAT GTACATGGTG GTCCTGTTGC
    CGAGTATTTG ATCCGCTGAT ATTCAGCTGT CTATGTGTAT TAATGGCTAT ATTTAGTGCA
    GGGCACTTAA TTGGACTTTA TCTGTACCAG TTGCAGTTTT TTCACGAGAT TATTCCACCA
    AATGATTTCT ATGCCAGGTT GTTTGGTGTT ACTTCATTAG TTCAAACCGA CTGCTGTAAC
    ACTTGGAGAT TTTTTGCAAA CAACAGTCTA CCATGGAACC AGTATACCAA TCCTGTCATG
    CTTTTAGTAC TGTATTACAC GCTGGCAACT CTCATACGCC TTTGGCTACA GGAACCTATT
    AATGTAGAGG AAGAGAAGGA GGAAGACTGT AGCCCAGCAG CACTTACTGC AGAAAGAAGA
    CGTAGTTTGT GGTACTCTAG CCACTACACA ACGGATGAAA GACAACTTCT GTCCATGACT
    CAAGATGACT ACAAGCCATC TGATGTTTTA CTAGTAACTG TAAATGGGAC TCCCGTAGAT
    TACTGTTCAC TAAATTCATC ACTTCCGATA GAAAATGGTC CAACTAAAAT TGACATGTAT
    TCAACACCGC AATATAAATG GGACCATGAA GATGAAACTT CAGAAAAGAA AGAAGAGGAA
    GAGCAAGAAG AAGAACCCAA TGAAGAGGTT GCAGAAGAGA AAGAAAACAC TAAAGTTCAT
    GTAATGGTGG TGGCATTTCA GTTCATTATG AAGCAAAGCT ATATATGTGC ACTAATTTCA
    ATGATGGCCT GGAGCATCAC ATACCACAGC TGGTTGACTT TTGTTTTGTT AATCTGGTCT
    TGTGCTCTCT GGATGGTCCG AAATAGGAGA AAATATGCTA TGCTTAGTTC ACCTTTTATG
    GTTATGTATG GTAATTTACT TTTAACATTA CAATACATAT GGAGCATAGC CTTACAAAGT
    GATGAACTTC CAGAAGTTAT GGGTCTCCTT GAGAGGAAAA AGCCCGAAGA GCTTGTATCG
    AAGATCATTT TCACAGTTAC ATTTTGGCTT TTGCTCAGAC AACACCTTAC AGAGCAAAAG
    GCATTAAAGC AAAAAGAATT AGCATTATCT GAAATAAAAG TTGGAAGTGA AGACAAAGAA
    GGTGAAGTGC AAGAAGAAGA AGAAGAAGTG GAGGATGAAA ATGATATCAT GAAGGTCCTC
    GGAAAGCTAG TGCAGGCTAT GTTTGTCAAG TACTGGATTT ATGTCTGTGG CGGCATGTTT
    TTTTTTGTCA GCTTTGAAGG AAAAATAGTT ATGTACAAAA TTATCTACAT GATGCTCTTC
    CTATTCTGTG TAGCTCTGTA TCAGGTGCAC TATGAATGGT GGAGACGAAT TTTAAAATAT
    TTTTGGATGT CTGTGGTTGT TTACACCATG TTGGTGCTTG TTTTTATTTA CACTTACCAA
    TTTAAATCAT TTCCTGGCCT TTGGATGAAT ATGACAGGGC TGGATAAGGA AAAGTTAGCA
    GATCTTGGTT TAGAGACATT TAACTTGACT GACCTATTTA CTCGTATATT TATCCCAACC
    TCATTTTTGT TAGTGTGCAT TTTGCACCTA CATTACTTCC ATGACCGTTT TCTCCAGCTC
    ACTGACCTAA AGGCTGTAAC CAGCAAGCAG GACAGCACAA TTTACAGATT GGTGCATCCA
    GAAGGAAGTT TGCCTGATAT AACGATGATG AACTTAACAA CAGAATCTGA AATGCTTGAG
    GAAAAGCGGT TGGAAATTTA TGACGAAAAA AAAACTCATG ACCAAGACTC AGATGATGAA
    TGGGATAAAG GGGATAGCAA AGAACCAGGT CATTCTGATG ATGAAGCTGA GGATGAGGAA
    TCAGAAGAAG AAGAAACAAC AACAGAGTCC AGGAACAAGT GGCATCTAGT GATAGATCGC
    TTAACGGTTC TTTTCCTAAA GTTTCTGGAA TATTTTCACA AGTTTCAGGT CTTTGTTTGG
    TGGCTTCTGG AGCTTCACAT GATAAAGATT GTTTCTTCAT ACATCATTTG GCTTACTATA
    AAGGAGGTTT CACTGCTCAA TTATGTATTT TTTATTGCCT GGGCCTTCGC TTTGCCCTAC
    TCACAATTCC GGCCATTGGC ATCAAGTGTT TGCACAGTCT GGACATGTGT CATCATCATA
    TGTAAAATGT TGTACCAGCT AGCCTCCATT GACCCTGCAA CCTTTTCCAG CAACTGTTTA
    ATGCCACAGC TAAACGAAAC AACTGCACGT AACCAAACTG AGCTTAATTC TTCTGTGCTT
    TACAATTCCT CTGTTGATCC TTCTAATTGG GTTGGACTAA AGAAGTCAAC TCCACTCCTT
    GCTTATTTGC GGAACAATTT GCTGATGTTG GCCATGTTAG CTTTTGAAGT AACCATTTAC
    CGGCATCAGG AGTATTACAG GTTGCGAAAC AATCTCACCA CACCTGCAAC AAAAACCATC
    TTTCATGACA TCACAAGACT TCACTTGGAT GATGGACTCA TAAATAGTGC CAAGTATTTT
    GTGAACTACT TTTTCTACAA GTTTGGATTG GAAACATGTT TCTTACTGTC GGTAAATGTA
    ATCGGTCAGA GGATGGACTT TTTTGCCATG GTTCATGCAC TTTGGCTTTT TGGCGTATTA
    TACCGACGCA GAAGGAAAGC GATTGCAGAA ACCTGGCACA GATATTGCTG TTTTCTTGCT
    TGCATTATGG CATTCCAGTA TTTTATATGT ATTGGCATCC CCCCTGCTGC ATGTAAAGAC
    TATCCTTGGA GACAACCTGG AGCCAATTTT AATTCTAACA TAATAAAGTG GTTATACTTT
    CCAGACTTTA TTGTGAGGCC TAGTCCTCTG TTTCTTGTCT ATGATTTTTT GCTTTTGCTT
    TGTGCGTCCC TTCAAAGAGA AGTGTTTGAG GAAGAAAATA AGGCAGCAGT TCGAATCATG
    GCTGGTGACA ATGTAGAAAT TTGCATGAAC CTTGATGCTG CATCTTTTAG CCAACATAAT
    CCTGTACCTG ATTTCATATA CTGCAGATCT TATTTGGACA TGGGTAAAGT TATAATGTTT
    AGTTACCTTT TCTGGTTTGT GCTCACCATC ATCTTCATCA CGGGAACTAC ACGGATCAGC
    ATATTCTGCA TGGGCTACTT GGTAGCTTGC TTCTATTTTC TATTATTTGG TGGAGATTTG
    CTGTTAAAAC CTATAAGAAT CATCCTACGC TATTGGGATT GGCTGATTGC TTACAATGTT
    TTTGTCATTA CAATGAAAAA TGTATTATCA ATAGGAGCAT GTGGCTACAT TGAATATTTA
    ATCAAAGACA GTTGTTGGTT AATTCAGGCA TTCAGTCTTT CTTGTACAGT GAAAGGTTAT
    TTTATCCCCC AAAACACACC TGACTGTCAG TTGCCAAGCG GAGAGGCTGG AATCATTTGG
    GACAGTATAT GTTTTGCTTT CCTTTTATTG CAAAGAAGAG TGTTTATGAG CTATTACTTC
    TTACATGTGG TTGCAGACAT CAAATCATCT CAGATTCTAG CATCAAGAGG TGCTGAACTG
    TTCCAAGCTA CAATTGTAAA AGCTGTAAAA GCAAGAATTG AAGAAGAAAA AAAATCTATG
    GACCAGCTCA AGAGACAGAT GGACCACATT AAAGCCAGGC AAGAAAAATA TAAAAAAGGT
    AAAGAAAAAA TGCTGAGTCT TACACAGGAT GGAGAAGGAC AAGAAGTTCA AAAACTAGAT
    GATGACGATG AAGAAGGAGA CAAAGCAGAC AAAGAGAAAG CCAAGGGCAA AAAAAAGCAG
    TGGTGGCGAC CTTGGGTTGA TCATGCTTCC ATGGTGAAAA GCGGTGATTA TTATTTGTTT
    GAGACGGACA GTGAAGAAGA AGATGAGGAT GACAAAAAAG AAGAAGAGCC TAGAAGAAAA
    TCTGCCTTCC AGTTTTTGTA CCAAGCATGG ATTACTGATC CAAAAACAGC CCTTAAGCAA
    AGAAGCATGG AAGCCAAAGA AGAAAAGGCA AGGGAAATGA GGCGAAAGGG CATTGCTGAA
    GGTGATATTA TTGTAGAATG TGGGGATAAA GAAGAAGAAA AAGAGCAAGG AAAAAAGAAA
    TCAGATGGCC CAGACAATAT CTTCAAAAGA ATATTTAACA TCCTGAAGTT TACTTGGGTC
    CTCTTCCTTG CTACAATCGA TAGTTTTACG GCTTGGCTAA ACTCCATTTC AAGAGAACAT
    ATTGATATCT CCAAAGTTCT AAGCATAGAA CGTTGCATGC TGACTAGAGA GATAAAAAAG
    GGCAATGTGC CTACTCGAGA AAGCATCCAC ATGTATTACC AAAATCAAAT AATGATGAGC
    CTTTCTAGAG AGTCTGGACT TGACAGAATT GATGACCAAC CTGGTTCATC GTCCAGTCAC
    CATAGCCATG ACAAGATGGC GAGTAAAAGG ATGGACAGTG CAGACTCAGC TGTATCTCAT
    GATAGCATAT CAAGTGAGCC AACTCAGTGC ACCATGGTCT ACTCACGGCA GGGCACAACA
    GAAACTATTG AGGAAGTGGA AGATGAAGAG GAGGAAGAAA GCAAAAGTCC TTTATCAAAG
    ATAGAAGCCG AGGAAGAAGA ATATGACATG GATTTGGAAG GGACAAGCTA TACTCCGGAC
    ACAGAATTTC CCCTGTACGC CACTACAGAG GGGGATCTTC CTCCTTCGTA CAGTAAAGCC
    GGCAGCTTCG AACAACTTAC ATTTGTATCT CAGGACTCGG CTGACAATTT AATGGTTCTA
    AGCCCAGATG AAAGTCGTAC TGATAAGCTA CATGATCATA TTCTTCCACC TCTTACACAT
    GAACTGACTG CTAGCGAGCT GCTTCTAAAC AAAATGTTTT ATGATGAAGA GTTAGAAGAC
    TCTGAAAAAT TTTATACTAC TCAACCACGC CCGCTGCAGC TTATTTATGC CTTGTATAAT
    ACATTGGTGG CCAGATCAGA AATGGTGTGC TATTTTGTGA TTATTCTTAA TCACATGATT
    TCAGCTTCTA TGATCACATT GGTACTGCCC ATCCTTATAT TCCTTTGGGC TATGCTTTCT
    GTCCCAAGGC CCAGTAAGAG ATTTTGGATG ACAGCTATTG TATATACAGA GGTGACAATT
    GTTATTAAAT ATTTTTTCCA ATTTGGATTC TTCCCATGGA ATAAAAATGT GGATTATACA
    AAGGACAAAC CTTACTTCCC ACCTAACATA ATGGGCATTG AGAAGAAAGA AGGTTATGTG
    CACTATGACC TGGTGCAACT GCTTGCTCTG TTTTTCCATA GATCTATTTT AAAGTGTCAT
    GGGCTTTGGG ATGAAGATGA TGTCACTGAA AAAGCCACAG ATAAAGACGA CACGGATGGA
    GAGATATCAG AGGCATCAGG AAGAAGGGAT TCCGTTGGCT CATTAAAATC TGTCAATCTA
    GCTGCGTCTG TGGAGTCAGT CCATGTGCAC TTCCCTGAGG AAAAGACCGC TATCAGGAGA
    AAAAGCACAA GCAGTGGATC GCAAATTTCC CGAAGGTCAA GTTATTCCTC GCACAGGTCT
    AAAAGAGGCA GCACCAGTAC TCGGAACAGC AGTCAAAAAG GAAGCAGCAT TCCCAGTATT
    AAACTGAAAT CTAGAAAGGA ACTTATACTG GAAAAACTCA GAGAACAGCT CATTAAAGCA
    AAAGCATTTA CGATCAAGAC GATACTACAA ATCTATGTCC CGATCAGACA GTTTTTTTAC
    AATCTTATAC ATCCTGAATA CAGTGCTGTG ACTGATGTCT ATGTGCTGAT GTTCCTGGCA
    GACACTGTAG ATTTCATCAT TATTGTTTTT GGATTTTGGG CGTTTGGAAA ACACTCCGCT
    GCAGCAGATA TTACATCCTC TCTATCAGAA GACCAAGTCC CAGAAGCATT TTTGGTAATG
    GTGCTTATCC AGTTTGGAAC CATGGTTGTT GATCGTGCTT TGTATCTTCG CAAAACAGTC
    ATGGGAAAAG TTATTTTCCA AGTTATCCTT GTATTTGGAA TCCATTTTTG GATGTTCTTC
    ATCTTGCCAG GGGTAACAGA AAGGAAATTT AGTCAGAACA CTGTTGCCCA GCTGTGGTAT
    TTTGTCAAGT GCATTTATTT TGGATTGTCG GCATATCAGA TACGTTGTGG ATATCCAACT
    CGGGTGCTTG GTAACTTCCT TACTAAAAGC TACAATTATG TCAACCTGTT CCTTTTCCAA
    GGCTTTCGCC TCGTTCCATT TCTTACGGAG CTGCGAGCTG TTATGGACTG GGTATGGACT
    GACACCACTC TCAGTCTTTC CAGCTGGATC TGTGTAGAGG ACATTTATGC ACATATATTT
    ATACTAAAAT GCTGGCGGGA GTCAGAAAAG AGATATCCCC AGCCACGAGG CCAAAAGAAG
    AAAAAGGTTG TGAAATACGG AATGGGTGGA ATGATAATAG TTCTGCTGAT TTGTATCGTG
    TGGTTTCCTT TGCTCTTCAT GTCCTTGATT AAATCAGTTG CTGGGGTCAC TAACAAGCCC
    CTAGATGTGT CCATTACAAT AACCCTTGGA GGTTATCAGC CTATTTTTAC CATGAGTGCA
    CAACAAAATC AGCTGCAAGG CTTGAATGAC AATGAATTTA AGCATTTACA TAATATATAT
    AAAGGCAATA CACCTGCCAT GCAGTTTCTG GAATCCTATA TGCAGGAAGA CATCACCATA
    GCAGGACTGG AAGGCAATTC CAACTCTCTG TGGACCATCA GCCCTCCGAG CAGGACAATG
    ATGATAGAAA GACTTATCCA GGAACCTGAC TTCACAGCGG TCATTTCTTG GTCCATTCGA
    AGAAATGTGA GTCTTGGAGC TAAGGCAGAG GTAACAGGTG AAAAATATAC AATATCACTG
    GCAGGTCAAA CTCGGAGGGA ACTTGCGGAT ATGATGAACA GTTCATCTCC CAATAAGACA
    GTGGAACTGA TAAAGATATA CCCATTGTGT TTAAAAGCAC CAAGTGATGT TACTGCAAAA
    CCCATCCTTC AGCTTCTTAA GAATCAAGAG TTTGTGAATA TTAGCGTTTT ACTAAGAAAA
    GAAAACACAT CAGAAGGGGT TCGAGAATGG TGGGTAGTGA ATCAAAGCCA TAAAGAGTTC
    ATTAAGAAGG ATGGCCTGGA ACTATGGGTT TTCAGTGACA AAGTCAGTCC TCCAAGTCTT
    GGATTCTTGG CAGGCTATGG AATAATGGGA CTATATGCCT CTGTAGTTCT CGTAATAGGA
    AAGTTTGTGC GTGAGTTTTT CAGCGGAATT TCGCACTCCA TCATGTTTGA GGAGCTTCCC
    AACGTTGACC GTATACTGAA GCTGTGCACA GACATTTTCC TTGTGAGAGA GACTGGAGAG
    TTGGAACTGG AGGAAGACCT GTATGCCAAA TTAATATTTT TGTACCGCTC CCCAGAAACT
    ATGATCAAAT GGACAAGAGA GAAAGATAAA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012820819.1
    CDS612..8744
    Translation

    Target ORF information:

    RefSeq Version XM_012965365.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis piezo-type mechanosensitive ion channel component 2 (piezo2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012965365.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    ATGGCATCGG AAGTGGTGTG TGGGCTCATC TTCAGATTTC TTCTCCCACT ATGCCTGGTT 
    GTAGCATGCA TGTTCCGGTA CAATGGAATA TCTTTTGTCT ACCTCATCTA CCTCTTGCTT
    ATACCTTTAT TTTCGGAGCC TACCAAAATA ACAATGCAAG GTCATACAGG ACGGTTATTA
    AAATCCTTAT GTGTTTCCAG TCTGTCATTT CTGCTGCTGC ACATCATCTT TCAAATTACC
    GTATACAGCC TCGAGGCCAG AAAACACATT GAACCACATT TTAACTGTTC AACATGGGAG
    AAAGCAATAC GACAAATTGG TTTTGAAAGT GTAAAAGGAG CAGATGCTGG TAATGGAATC
    CGCGTGTTTG CACCAGATAT CGGAATGTTT ATTGCAAGCT TGGCTACCCT GTTGGTTTGT
    AGAAAACTAG TTCAGAAAAC AGTTACTGAA GAGGACGCAC AATACAACCC CCAGTTTGTA
    AATGAAGAAA TGGCAGAAGA AGGAAAGGTG GACCTGGATG AAGCGTTAAT GTATGAGGAA
    GACTTTGATG GAGAAGAGGA TGAAATTGAG GCAGCTGAAG AAAGCACAAA ATTAAAAATT
    CTTCGCAGAA TTGCTTCATT TGCATCCGTA CTTAAAGAGT TTATTGGCAA CATTATAACC
    ACCGCTGGGA AAGTAGTTAT TACAATTTTA CTTGGTTTAA CAGGCATGAT GTTGCCGTCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT TTGGGGCTAT GTACATGGTG GTCCTGTTGC
    CGAGTATTTG ATCCGCTGAT ATTCAGCTGT CTATGTGTAT TAATGGCTAT ATTTAGTGCA
    GGGCACTTAA TTGGACTTTA TCTGTACCAG TTGCAGTTTT TTCACGAGAT TATTCCACCA
    AATGATTTCT ATGCCAGGTT GTTTGGTGTT ACTTCATTAG TTCAAACCGA CTGCTGTAAC
    ACTTGGAGAT TTTTTGCAAA CAACAGTCTA CCATGGAACC AGTATACCAA TCCTGTCATG
    CTTTTAGTAC TGTATTACAC GCTGGCAACT CTCATACGCC TTTGGCTACA GGAACCTATT
    AATGTAGAGG AAGAGAAGGA GGAAGACTGT AGCCCAGCAG CACTTACTGC AGAAAGAAGA
    CGTAGTTTGT GGTACTCTAG CCACTACACA ACGGATGAAA GACAACTTCT GTCCATGACT
    CAAGATGACT ACAAGCCATC TGATGTTTTA CTAGTAACTG TAAATGGGAC TCCCGTAGAT
    TACTGTTCAC TAAATTCATC ACTTCCGATA GAAAATGGTC CAACTAAAAT TGACATGTAT
    TCAACACCGC AATATAAATG GGACCATGAA GATGAAACTT CAGAAAAGAA AGAAGAGGAA
    GAGCAAGAAG AAGAACCCAA TGAAGAGGTT GCAGAAGAGA AAGAAAACAC TAAAGTTCAT
    GTAATGGTGG TGGCATTTCA GTTCATTATG AAGCAAAGCT ATATATGTGC ACTAATTTCA
    ATGATGGCCT GGAGCATCAC ATACCACAGC TGGTTGACTT TTGTTTTGTT AATCTGGTCT
    TGTGCTCTCT GGATGGTCCG AAATAGGAGA AAATATGCTA TGCTTAGTTC ACCTTTTATG
    GTTATGTATG GTAATTTACT TTTAACATTA CAATACATAT GGAGCATAGC CTTACAAAGT
    GATGAACTTC CAGAAGTTAT GGGTCTCCTT GAGAGGAAAA AGCCCGAAGA GCTTGTATCG
    AAGATCATTT TCACAGTTAC ATTTTGGCTT TTGCTCAGAC AACACCTTAC AGAGCAAAAG
    GCATTAAAGC AAAAAGAATT AGCATTATCT GAAATAAAAG TTGGAAGTGA AGACAAAGAA
    GGTGAAGTGC AAGAAGAAGA AGAAGAAGTG GAGGATGAAA ATGATATCAT GAAGGTCCTC
    GGAAAGCTAG TGCAGGCTAT GTTTGTCAAG TACTGGATTT ATGTCTGTGG CGGCATGTTT
    TTTTTTGTCA GCTTTGAAGG AAAAATAGTT ATGTACAAAA TTATCTACAT GATGCTCTTC
    CTATTCTGTG TAGCTCTGTA TCAGGTGCAC TATGAATGGT GGAGACGAAT TTTAAAATAT
    TTTTGGATGT CTGTGGTTGT TTACACCATG TTGGTGCTTG TTTTTATTTA CACTTACCAA
    TTTAAATCAT TTCCTGGCCT TTGGATGAAT ATGACAGGGC TGGATAAGGA AAAGTTAGCA
    GATCTTGGTT TAGAGACATT TAACTTGACT GACCTATTTA CTCGTATATT TATCCCAACC
    TCATTTTTGT TAGTGTGCAT TTTGCACCTA CATTACTTCC ATGACCGTTT TCTCCAGCTC
    ACTGACCTAA AGGCTGTAAC CAGCAAGCAG GACAGCACAA TTTACAGATT GGTGCATCCA
    GAAGGAAGTT TGCCTGATAT AACGATGATG AACTTAACAA CAGAATCTGA AATGCTTGAG
    GAAAAGCGGT TGGAAATTTA TGACGAAAAA AAAACTCATG ACCAAGACTC AGATGATGAA
    TGGGATAAAG GGGATAGCAA AGAACCAGGT CATTCTGATG ATGAAGCTGA GGATGAGGAA
    TCAGAAGAAG AAGAAACAAC AACAGAGTCC AGGAACAAGT GGCATCTAGT GATAGATCGC
    TTAACGGTTC TTTTCCTAAA GTTTCTGGAA TATTTTCACA AGTTTCAGGT CTTTGTTTGG
    TGGCTTCTGG AGCTTCACAT GATAAAGATT GTTTCTTCAT ACATCATTTG GCTTACTATA
    AAGGAGGTTT CACTGCTCAA TTATGTATTT TTTATTGCCT GGGCCTTCGC TTTGCCCTAC
    TCACAATTCC GGCCATTGGC ATCAAGTGTT TGCACAGTCT GGACATGTGT CATCATCATA
    TGTAAAATGT TGTACCAGCT AGCCTCCATT GACCCTGCAA CCTTTTCCAG CAACTGTTTA
    ATGCCACAGC TAAACGAAAC AACTGCACGT AACCAAACTG AGCTTAATTC TTCTGTGCTT
    TACAATTCCT CTGTTGATCC TTCTAATTGG GTTGGACTAA AGAAGTCAAC TCCACTCCTT
    GCTTATTTGC GGAACAATTT GCTGATGTTG GCCATGTTAG CTTTTGAAGT AACCATTTAC
    CGGCATCAGG AGTATTACAG GTTGCGAAAC AATCTCACCA CACCTGCAAC AAAAACCATC
    TTTCATGACA TCACAAGACT TCACTTGGAT GATGGACTCA TAAATAGTGC CAAGTATTTT
    GTGAACTACT TTTTCTACAA GTTTGGATTG GAAACATGTT TCTTACTGTC GGTAAATGTA
    ATCGGTCAGA GGATGGACTT TTTTGCCATG GTTCATGCAC TTTGGCTTTT TGGCGTATTA
    TACCGACGCA GAAGGAAAGC GATTGCAGAA ACCTGGCACA GATATTGCTG TTTTCTTGCT
    TGCATTATGG CATTCCAGTA TTTTATATGT ATTGGCATCC CCCCTGCTGC ATGTAAAGAC
    TATCCTTGGA GACAACCTGG AGCCAATTTT AATTCTAACA TAATAAAGTG GTTATACTTT
    CCAGACTTTA TTGTGAGGCC TAGTCCTCTG TTTCTTGTCT ATGATTTTTT GCTTTTGCTT
    TGTGCGTCCC TTCAAAGAGA AGTGTTTGAG GAAGAAAATA AGGCAGCAGT TCGAATCATG
    GCTGGTGACA ATGTAGAAAT TTGCATGAAC CTTGATGCTG CATCTTTTAG CCAACATAAT
    CCTGTACCTG ATTTCATATA CTGCAGATCT TATTTGGACA TGGGTAAAGT TATAATGTTT
    AGTTACCTTT TCTGGTTTGT GCTCACCATC ATCTTCATCA CGGGAACTAC ACGGATCAGC
    ATATTCTGCA TGGGCTACTT GGTAGCTTGC TTCTATTTTC TATTATTTGG TGGAGATTTG
    CTGTTAAAAC CTATAAGAAT CATCCTACGC TATTGGGATT GGCTGATTGC TTACAATGTT
    TTTGTCATTA CAATGAAAAA TGTATTATCA ATAGGAGCAT GTGGCTACAT TGAATATTTA
    ATCAAAGACA GTTGTTGGTT AATTCAGGCA TTCAGTCTTT CTTGTACAGT GAAAGGTTAT
    TTTATCCCCC AAAACACACC TGACTGTCAG TTGCCAAGCG GAGAGGCTGG AATCATTTGG
    GACAGTATAT GTTTTGCTTT CCTTTTATTG CAAAGAAGAG TGTTTATGAG CTATTACTTC
    TTACATGTGG TTGCAGACAT CAAATCATCT CAGATTCTAG CATCAAGAGG TGCTGAACTG
    TTCCAAGCTA CAATTGTAAA AGCTGTAAAA GCAAGAATTG AAGAAGAAAA AAAATCTATG
    GACCAGCTCA AGAGACAGAT GGACCACATT AAAGCCAGGC AAGAAAAATA TAAAAAAGGT
    AAAGAAAAAA TGCTGAGTCT TACACAGGAT GGAGAAGGAC AAGAAGTTCA AAAACTAGAT
    GATGACGATG AAGAAGGAGA CAAAGCAGAC AAAGAGAAAG CCAAGGGCAA AAAAAAGCAG
    TGGTGGCGAC CTTGGGTTGA TCATGCTTCC ATGGTGAAAA GCGGTGATTA TTATTTGTTT
    GAGACGGACA GTGAAGAAGA AGATGAGGAT GACAAAAAAG AAGAAGAGCC TAGAAGAAAA
    TCTGCCTTCC AGTTTTTGTA CCAAGCATGG ATTACTGATC CAAAAACAGC CCTTAAGCAA
    AGAAGCATGG AAGCCAAAGA AGAAAAGGCA AGGGAAATGA GGCGAAAGGG CATTGCTGAA
    GGTGATATTA TTGTAGAATG TGGGGATAAA GAAGAAGAAA AAGAGCAAGG AAAAAAGAAA
    TCAGATGGCC CAGACAATAT CTTCAAAAGA ATATTTAACA TCCTGAAGTT TACTTGGGTC
    CTCTTCCTTG CTACAATCGA TAGTTTTACG GCTTGGCTAA ACTCCATTTC AAGAGAACAT
    ATTGATATCT CCAAAGTTCT AAGCATAGAA CGTTGCATGC TGACTAGAGA GATAAAAAAG
    GGCAATGTGC CTACTCGAGA AAGCATCCAC ATGTATTACC AAAATCAAAT AATGATGAGC
    CTTTCTAGAG AGTCTGGACT TGACAGAATT GATGACCAAC CTGGTTCATC GTCCAGTCAC
    CATAGCCATG ACAAGATGGC GAGTAAAAGG ATGGACAGTG CAGACTCAGC TGTATCTCAT
    GATAGCATAT CAAGTGAGCC AACTCAGTGC ACCATGGTCT ACTCACGGCA GGGCACAACA
    GAAACTATTG AGGAAGTGGA AGATGAAGAG GAGGAAGAAA GCAAAAGTCC TTTATCAAAG
    ATAGAAGCCG AGGAAGAAGA ATATGACATG GATTTGGAAG GGACAAGCTA TACTCCGGAC
    ACAGAATTTC CCCTGTACGC CACTACAGAG GGGGATCTTC CTCCTTCGTA CAGTAAAGCC
    GGCAGCTTCG AACAACTTAC ATTTGTATCT CAGGACTCGG CTGACAATTT AATGGTTCTA
    AGCCCAGATG AAAGTCGTAC TGATAAGCTA CATGATCATA TTCTTCCACC TCTTACACAT
    GAACTGACTG CTAGCGAGCT GCTTCTAAAC AAAATGTTTT ATGATGAAGA GTTAGAAGAC
    TCTGAAAAAT TTTATACTAC TCAACCACGC CCGCTGCAGC TTATTTATGC CTTGTATAAT
    ACATTGGTGG CCAGATCAGA AATGGTGTGC TATTTTGTGA TTATTCTTAA TCACATGATT
    TCAGCTTCTA TGATCACATT GGTACTGCCC ATCCTTATAT TCCTTTGGGC TATGCTTTCT
    GTCCCAAGGC CCAGTAAGAG ATTTTGGATG ACAGCTATTG TATATACAGA GGTGACAATT
    GTTATTAAAT ATTTTTTCCA ATTTGGATTC TTCCCATGGA ATAAAAATGT GGATTATACA
    AAGGACAAAC CTTACTTCCC ACCTAACATA ATGGGCATTG AGAAGAAAGA AGGTTATGTG
    CACTATGACC TGGTGCAACT GCTTGCTCTG TTTTTCCATA GATCTATTTT AAAGTGTCAT
    GGGCTTTGGG ATGAAGATGA TGTCACTGAA AAAGCCACAG ATAAAGACGA CACGGATGGA
    GAGATATCAG AGGCATCAGG AAGAAGGGAT TCCGTTGGCT CATTAAAATC TGTCAATCTA
    GCTGCGTCTG TGGAGTCAGT CCATGTGCAC TTCCCTGAGG AAAAGACCGC TATCAGGAGA
    AAAAGCACAA GCAGTGGATC GCAAATTTCC CGAAGGTCAA GTTATTCCTC GCACAGGTCT
    AAAAGAGGCA GCACCAGTAC TCGGAACAGC AGTCAAAAAG GAAGCAGCAT TCCCAGTATT
    AAACTGAAAT CTAGAAAGGA ACTTATACTG GAAAAACTCA GAGAACAGCT CATTAAAGCA
    AAAGCATTTA CGATCAAGAC GATACTACAA ATCTATGTCC CGATCAGACA GTTTTTTTAC
    AATCTTATAC ATCCTGAATA CAGTGCTGTG ACTGATGTCT ATGTGCTGAT GTTCCTGGCA
    GACACTGTAG ATTTCATCAT TATTGTTTTT GGATTTTGGG CGTTTGGAAA ACACTCCGCT
    GCAGCAGATA TTACATCCTC TCTATCAGAA GACCAAGTCC CAGAAGCATT TTTGGTAATG
    GTGCTTATCC AGTTTGGAAC CATGGTTGTT GATCGTGCTT TGTATCTTCG CAAAACAGTC
    ATGGGAAAAG TTATTTTCCA AGTTATCCTT GTATTTGGAA TCCATTTTTG GATGTTCTTC
    ATCTTGCCAG GGGTAACAGA AAGGAAATTT AGTCAGAACA CTGTTGCCCA GCTGTGGTAT
    TTTGTCAAGT GCATTTATTT TGGATTGTCG GCATATCAGA TACGTTGTGG ATATCCAACT
    CGGGTGCTTG GTAACTTCCT TACTAAAAGC TACAATTATG TCAACCTGTT CCTTTTCCAA
    GGCTTTCGCC TCGTTCCATT TCTTACGGAG CTGCGAGCTG TTATGGACTG GGTATGGACT
    GACACCACTC TCAGTCTTTC CAGCTGGATC TGTGTAGAGG ACATTTATGC ACATATATTT
    ATACTAAAAT GCTGGCGGGA GTCAGAAAAG AGATATCCCC AGCCACGAGG CCAAAAGAAG
    AAAAAGGTTG TGAAATACGG AATGGGTGGA ATGATAATAG TTCTGCTGAT TTGTATCGTG
    TGGTTTCCTT TGCTCTTCAT GTCCTTGATT AAATCAGTTG CTGGGGTCAC TAACAAGCCC
    CTAGATGTGT CCATTACAAT AACCCTTGGA GGTTATCAGC CTATTTTTAC CATGAGTGCA
    CAACAAAATC AGCTGCAAGG CTTGAATGAC AATGAATTTA AGCATTTACA TAATATATAT
    AAAGGCAATA CACCTGCCAT GCAGTTTCTG GAATCCTATA TGCAGGAAGA CATCACCATA
    GCAGGACTGG AAGGCAATTC CAACTCTCTG TGGACCATCA GCCCTCCGAG CAGGACAATG
    ATGATAGAAA GACTTATCCA GGAACCTGAC TTCACAGCGG TCATTTCTTG GTCCATTCGA
    AGAAATGTGA GTCTTGGAGC TAAGGCAGAG GTAACAGGTG AAAAATATAC AATATCACTG
    GCAGGTCAAA CTCGGAGGGA ACTTGCGGAT ATGATGAACA GTTCATCTCC CAATAAGACA
    GTGGAACTGA TAAAGATATA CCCATTGTGT TTAAAAGCAC CAAGTGATGT TACTGCAAAA
    CCCATCCTTC AGCTTCTTAA GAATCAAGAG TTTGTGAATA TTAGCGTTTT ACTAAGAAAA
    GAAAACACAT CAGAAGGGGT TCGAGAATGG TGGGTAGTGA ATCAAAGCCA TAAAGAGTTC
    ATTAAGAAGG ATGGCCTGGA ACTATGGGTT TTCAGTGACA AAGTCAGTCC TCCAAGTCTT
    GGATTCTTGG CAGGCTATGG AATAATGGGA CTATATGCCT CTGTAGTTCT CGTAATAGGA
    AAGTTTGTGC GTGAGTTTTT CAGCGGAATT TCGCACTCCA TCATGTTTGA GGAGCTTCCC
    AACGTTGACC GTATACTGAA GCTGTGCACA GACATTTTCC TTGTGAGAGA GACTGGAGAG
    TTGGAACTGG AGGAAGACCT GTATGCCAAA TTAATATTTT TGTACCGCTC CCCAGAAACT
    ATGATCAAAT GGACAAGAGA GAAAGATAAA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa60016
    Clone ID Related Accession (Same CDS sequence) XM_012965359.3
    Accession Version XM_012965359.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8469bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product piezo-type mechanosensitive ion channel component 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012965359.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGGCATCGG AAGTGGTGTG TGGGCTCATC TTCAGATTTC TTCTCCCACT ATGCCTGGTT 
    GTAGCGTGCA TGTTCCGGTA CAATGGAATA TCTTTTGTCT ACCTCATCTA CCTCTTGCTT
    ATACCTTTAT TTTCGGAGCC TACCAAAATA ACAATGCAAG GTCATACAGG ACGGTTATTA
    AAATCCTTAT GTGTTTCCAG TCTGTCATTT CTGCTGCTGC ACATCATCTT TCAAATTACC
    GTATACAGCC TCGAGGCCAG AAAACACATT GAACCACATT TTAACTGTTC AACATGGGAG
    AAAGCAATAC GACAAATTGG TTTTGAAAGT GTAAAAGGAG CAGATGCTGG TAATGGAATC
    CGCGTGTTTG CACCAGATAT CGGAATGTTT ATTGCAAGCT TGGCTACCCT GTTGGTTTGT
    AGAAAACTAG TTCAGAAAAC AGTTACTGAA GAGGACGCAC AATACAACCC CCAGTTTGTA
    AATGAAGAAA TGGCAGAAGA AGGAAAGGTG GACCTGGATG AAGCGTTAAT GTATGAGGAA
    GACTTTGATG GAGAAGAGGA TGAAATTGAG GCAGCTGAAG AAAGCACAAA ATTAAAAATT
    CTTCGCAGAA TTGCTTCATT TGCATCCGTA CTTAAAGAGT TTATTGGCAA CATTATAACC
    ACAGCTGGGA AAGTAGTTAT TACAATTTTA CTTGGTTTAA CAGGCATGAT GTTGCCGTCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT TTGGGGCTAT GCACATGGTG GTCCTGTTGC
    CGAGTATTTG ATCCGCTGAT ATTCAGCTGT CTATGTGTAT TAATGGCTAT ATTTAGTGCA
    GGGCACTTAA TTGGACTTTA TCTGTACCAG TTGCAGTTTT TTCACGAGAT TATTCCACCA
    AATGATTTCT ATGCCAGGTT GTTTGGTGTT ACTTCATTAG TTCAAACCGA CTGCTGTAAC
    ACTTGGAGAT TTTTTGCAAA CAACAGTCTA CCATGGAACC AGTATACCAA TCCTGTCATG
    CTTTTAGTAC TGTATTACAC GCTGGCAACT CTCATACGCC TTTGGCTACA GGAACCTATT
    AATGTAGAGG AAGAGAAGGA GGAAGACTGT AGCCCAGCAG CACTTACTGC AGAAAGAAGA
    CGTAGTTTGT GGTACTCTAG GCACTACACA ACGGATGAAA GACAACTTCT GTCCATGACT
    CAAGATGACT ACAAGCCATC TGATGTTTTA CTAGTAACTG TAAATGGGAC TCCCGTAGAT
    TACTGTTCAC TAAATTCATC ACTTCCGATA GAAAATGGTC CAACTAAAAT TGACATGTAT
    TCAACACCGC AATATAAATG GGACCATGAA GATGAAACTT CAGAAAAGAA AGAAGAGGAA
    GAGCAAGAAG AAGAACCCAA TGAAGAGGTT GCAGAAGAGA AAGAAAACAC TAAAGTTCAT
    GTAATGGTGG TGGCATTTCA GTTCATTATG AAGCAAAGCT ATATATGTGC ACTAATTTCA
    ATGATGGCCT GGAGCATCAC ATACCACAGC TGGTTGACTT TTGTTTTGTT AATCTGGTCT
    TGTGCTCTCT GGATGGTCCG AAATAGGAGA AAATATGCTA TGCTTAGTTC ACCTTTTATG
    GTTATGTATG GTAATTTACT TTTAACATTA CAATACATAT GGAGCATAGC CTTACAAAGT
    GATGAACTTC CAGAAGTTAT GGGTCTCCTT GAGAGAAAAA AGCCCGAAGA GCTTGTATCG
    AAGATCATTT TCACAGTTAC ATTTTGGCTT TTGCTCAGAC AACACCTTAC AGAGCAAAAG
    GCATTAAAGC AAAAAGAATT AGCATTATCT GAAATAAAAG TTGGAAGTGA AGACAAAGAA
    GGTGAAGTGC AAGAAGAAGA AGAAGAAGTG GAGGATGAAA ATGATATCAT GAAGGTCCTC
    GGAAAGCTAG TGCAGGCTAT GTTTGTCAAG TACTGGATTT ATGTCTGTGG CGGCATGTTT
    TTTTTTGTCA GCTTTGAAGG AAAAATAGTT ATGTACAAAA TTATCTACAT GATGCTCTTC
    CTATTCTGTG TAGCTCTGTA TCAGGTGCAC TATGAATGGT GGAGACGAAT TTTAAAATAT
    TTTTGGATGT CTGTGGTTGT TTACACCATG TTGGTGCTTG TTTTTATTTA CACTTACCAA
    TTTAAATCAT TTCCGGGCCT TTGGATGAAT ATGACAGGGC TGGATAAGGA AAAGTTAGCA
    GATCTTGGTT TAGAGACATT TAACTTGACT GACCTATTTA CTCGTATATT TATCCCAACC
    TCATTTTTGT TAGTGTGCAT TTTGCACCTA CATTACTTCC ATGACCGTTT TCTCCAGCTC
    ACTGACCTAA AGGCTGTAAC CAGCAAGCAG GACAGCACAA TTTACAGCCA TGCCAAAGTA
    AATGGTCGAG TTTACTTAAT AATAAATAGC ATCAAGCAAA AAATTCCAAT TCAGCAAAAT
    GAATTGGTGC ATCCAGAAGG AAGTTTGCCT GATATAACGA TGATGAACTT AACAACAGAA
    TCTGAAATGC TTGAGGAAAA GCGGTTGGAA ATTTATGACG AAAAAAAAAC TCATGACCAA
    GACTCAGATG ATGAATGGGA TAAAGGGGAT AGCAAAGAAC CAGGTCATTC TGATGATGAA
    GCTGAGGATG AGGAATCAGA AGAAGAAGAA ACAACAACAG AGTCCAGGAA CAAGTGGCAT
    CTAGTGATAG ATCGCTTAAC GGTTCTTTTC CTAAAGTTTC TGGAATATTT TCACAAGTTT
    CAGGTCTTTG TTTGGTGGCT TCTGGAGCTT CACATGATAA AGATTGTTTC TTCATACATC
    ATTTGGCTTA CTATAAAGGA GGTTTCACTG CTCAATTATG TATTTTTTAT TGCCTGGGCC
    TTCGCTTTGC CCTACTCACA ATTCCGGCCA TTGGCATCAA GTGTTTGCAC AGTCTGGACA
    TGTGTCATCA TCATATGTAA AATGTTGTAC CAGCTAGCCT CCATTGACCC TGCAACCTTT
    TCCAGCAACT GTTTAATGCC ACAGCTAAAC GAAACAACTG CACGTAACCA AACTGAGCTT
    AATTCTTCTG TGCTTTACAA TTCCTCTGTT GATCCTTCTA ATTGGGTTGG ACTAAAGAAG
    TCAACTCCAC TCCTTGCTTA TTTGCGGAAC AATTTGCTGA TGTTGGCCAT GTTAGCTTTT
    GAAGTAACCA TTTACCGGCA TCAGGAGTAT TACAGGTTGC GAAACAATCT CACCACACCT
    GCAACAAAAA CCATCTTTCA TGACATCACA AGACTTCACT TGGATGATGG ACTCATAAAT
    AGTGCCAAGT ATTTTGTGAA CTACTTTTTC TACAAGTTTG GATTGGAAAC ATGTTTCTTA
    CTGTCGGTAA ATGTAATCGG TCAGAGGATG GACTTTTTTG CCATGGTTCA TGCACTTTGG
    CTTTTTGGCG TATTATACCG ACGCAGAAGG AAAGCGATTG CAGAAACCTG GCACAGATAT
    TGCTGTTTTC TTGCTTGCAT TATGGCATTC CAGTATTTTA TATGTATTGG CATCCCCCCT
    GCTGCATGTA AAGACTATCC TTGGAGACAA CCTGGAGCCA ATTTTAATTC TAACATAATA
    AAGTGGTTAT ACTTTCCAGA CTTTATTGTG AGGCCTAGTC CTCTGTTTCT TGTCTATGAT
    TTTTTGCTTT TGCTTTGTGC GTCCCTTCAA AGAGAAGTGT TTGAGGAAGA AAATAAGGCA
    GCAGTTCGAA TCATGGCTGG TGACAATGTA GAAATTTGCA TGAACCTTGA TGCTGCATCT
    TTTAGCCAAC ATAATCCTGT ACCTGATTTC ATATACTGCA GATCTTATTT GGACATGGGT
    AAAGTTATAA TGTTTAGTTA CCTTTTCTGG TTTGTGCTCA CCATCATCTT CATCACGGGA
    ACTACACGGA TCAGCATATT CTGCATGGGC TACTTGGTAG CTTGCTTCTA TTTTCTATTA
    TTTGGTGGAG ATTTGCTGTT AAAACCTATA AGAATCATCC TACGCTATTG GGATTGGCTG
    ATTGCTTACA ATGTTTTTGT CATTACAATG AAAAATGTAT TATCAATAGG AGCATGTGGC
    TACATTGAAT ATTTAATCAA AGACAGTTGT TGGTTAATTC AGGCATTCAG TCTTTCTTGT
    ACAGTGAAAG GTTATTTTAT CCCCCAAAAC ACACCTGACT GTCAGTTGCC AAGCGGAGAG
    GCTGGAATCA TTTGGGACAG TATATGTTTT GCTTTCCTTT TATTGCAAAG AAGAGTGTTT
    ATGAGCTATT ACTTCTTACA TGTGGTTGCA GACATCAAAT CATCTCAGAT TCTAGCATCA
    AGAGGTGCTG AACTGTTCCA AGCTACAATT GTAAAAGCTG TAAAAGCAAG AATTGAAGAA
    GAAAAAAAAT CTATGGACCA GCTCAAGAGA CAGATGGACC ACATTAAAGC CAGGCAAGAA
    AAATATAAAA AAGGTAAAGA AAAAATGCTG AGTCTTACAC AGGATGGAGA AGGACAAGAA
    GTTCAAAAAC TAGATGATGA CGATGAAGAA GGAGACAAAG CAGACAAAGA GAAAGCCAAG
    GGCAAAAAAA AGCAGTGGTG GCGACCTTGG GTTGATCATG CTTCCATGGT GAAAAGCGGT
    GATTATTATT TGTTTGAGAC GGACAGTGAA GAAGAAGATG AGGATGACAA AAAAGAAGAA
    GAGCCTAGAA GAAAATCTGC CTTCCAGAGA GCTATTGGAA AGTTTGCATC AGCCATTATA
    GCCTTGCCAA AGTCTGTCAT TAAACTGCCT AAAACAATTC TTCAATATTT AATCAGAGCT
    GCAAAGTTTT TGTACCAAGC ATGGATTACT GATCCAAAAA CAGCCCTTAA GCAAAGAAGC
    ATGGAAGCCA AAGAAGAAAA GGCAAGGGAA ATGAGGCGAA AGGGCATTGC TGAAGGTGAT
    ATTATTGTAG AATGTGGGGA TAAAGAAGAA GAAAAAGAGC AAGGAAAAAA GAAATCAGAT
    GGCCCAGACA ATATCTTCAA AAGAATATTT AACATCCTGA AGTTTACTTG GGTCCTCTTC
    CTTGCTACAA TCGATAGTTT TACGGCTTGG CTAAACTCCA TTTCAAGAGA ACATATTGAT
    ATCTCCAAAG TTCTAAGCAT AGAACGTTGC ATGCTGACTA GAGAGATAAA AAAGGGCAAT
    GTGCCTACTC GAGAAAGCAT CCACATGTAT TACCAAAATC AAATAATGAT GAGCCTTTCT
    AGAGAGTCTG GACTTGACAG AATTGATGAC CAACCTGGTT CATCGTCCAG TCACCATAGC
    CATGACAAGA TGGCGAGTAA AAGGATGGAC AGTGCAGACT CAGCTGTATC TCATGATAGC
    ATATCAAGCT GCTGCACTGA AGCAACCATG CTATTCTCAA GGCAATCTAC ACTTGATGAT
    TTAGATGGAC CAGACTCTGT ACCAAAAACA AGTGAGCGTG CTCGACCTAG GCTTCGTAAA
    ATGTACAGCA TGGACATGTC TTCTTCATCT GCTGACAGTA TAGCTTCAAG TGAGCCAACT
    CAGTGCACCA TGGTCTACTC ACGGCAGGGC ACAACAGAAA CTATTGAGGA AGTGGAAGAT
    GAAGAGGAGG AAGAAAGCAA AAGTCCTTTA TCAAAGATAG AAGCCGAGGA AGAAGAATAT
    GACATGGATT TGGAAGGGAC AAGCTATACT CCGGACACAG AATTTCCCCT GTACGCCACT
    ACAGAGGGGG ATCTTCCTCC TTCGTACAGT AAAGCCGGCA GCTTCGAACA ACTTACATTT
    GTATCTCAGG ACTCGGCTGA CAATTTAATG GTTCTAAGCC CAGATGAAAG TCGTACTGAT
    AAGCTACATG ATCATATTCT TCCACCTCTT ACACATGAAC TGACTGCTAG CGAGCTGCTT
    CTAAACAAAA TGTTTTATGA TGAAGAGTTA GAAGACTCTG AAAAATTTTA TACTACTCAA
    CCACGCCCGC TGCAGCTTAT TTATGCCTTG TATAATACAT TGGTGGCCAG ATCAGAAATG
    GTGTGCTATT TTGTGATTAT TCTTAATCAC ATGATTTCAG CTTCTATGAT CACATTGGTA
    CTGCCCATCC TTATATTCCT TTGGGCTATG CTTTCTGTCC CAAGGCCCAG TAAGAGATTT
    TGGATGACAG CTATTGTATA TACAGAGGTG ACAATTGTTA TTAAATATTT TTTCCAATTT
    GGATTCTTCC CATGGAATAA AAATGTGGAT TATACAAAGG ACAAACCTTA CTTCCCACCT
    AACATAATGG GCATTGAGAA GAAAGAAGGT TATGTGCACT ATGACCTGGT GCAACTGCTT
    GCTCTGTTTT TCCATAGATC TATTTTAAAG TGCCACGGGC TTTGGGATGA AGATGATGTC
    ACTGAAAAAG CCACAGATAA AGACGACACG GATGGAGAGA TATCAGAGGC ATCAGGAAGA
    AGGGATTCCG TTGGCTCATT GAAATCTGTC AATCTAGCTG CGTCTGTGGA GTCAGTCCAT
    GTGCACTTCC CTGAGGAAAA GACCGCTATC AGGAGAAAAA GCACAAGCAG TGGATCGCAA
    ATTTCCCGAA GGTCAAGTTA TTCCTCGCAC AGGTCTAAAA GAGGCAGCAC CAGTACTCGG
    AACAGCAGTC AAAAAGGAAG CAGCATTCCC AGTATTAAAC TGAAATCTAG AAAGGAACTT
    ATACTGGAAA AACTCAGAGA ACAGCTCATT AAAGCAAAAG CATTTACGAT CAAGACGATA
    CTACAAATCT ATGTCCCGAT CAGACAGTTT TTTTACAATC TTATACATCC TGAATACAGT
    GCTGTGACTG ATGTCTATGT GCTGATGTTC CTGGCAGACA CTGTAGATTT CATCATTATT
    GTTTTTGGAT TTTGGGCGTT TGGAAAACAC TCCGCTGCAG CAGATATTAC ATCCTCTCTA
    TCAGAAGACC AAGTCCCAGA AGCATTTTTG GTAATGGTGC TTATCCAGTT TGGAACCATG
    GTTGTTGATC GTGCTTTGTA TCTTCGCAAA ACAGTCATGG GAAAAGTTAT TTTCCAAGTT
    ATCCTTGTAT TTGGAATCCA TTTTTGGATG TTCTTCATCT TGCCAGGGGT AACAGAAAGG
    AAATTTAGTC AGAACACTGT TGCCCAACTG TGGTATTTTG TCAAGTGCAT TTATTTTGGA
    TTGTCGGCAT ATCAGATACG TTGTGGATAT CCAACTCGGG TGCTTGGTAA CTTCCTTACT
    AAAAGCTACA ATTATGTCAA CCTGTTCCTT TTCCAAGGCT TTCGCCTCGT TCCATTTCTT
    ACGGAGCTGC GAGCTGTTAT GGACTGGGTA TGGACTGACA CCACTCTCAG TCTTTCCAGC
    TGGATCTGTG TAGAGGACAT TTATGCACAT ATATTTATAC TAAAATGCTG GCGGGAGTCA
    GAAAAGAGAT ATCCCCAGCC ACGAGGCCAA AAGAAGAAAA AGGTTGTGAA ATACGGAATG
    GGTGGAATGA TAATAGTTCT GCTGATTTGT ATCGTGTGGT TTCCTTTGCT CTTCATGTCC
    TTGATTAAAT CAGTTGCTGG GGTCACTAAC AAGCCCCTAG ATGTGTCCAT TACAATAACC
    CTTGGAGGTT ATCAGCCTAT TTTTACCATG AGTGCACAAC AAAATCAGCT GCAAGGCTTG
    AATGACAATG AATTTAAGCA TTTACATAAT ATATATAAAG GCAATACACC TGCCATGCAG
    TTTCTGGAAT CCTATATGCA GGAAGACATC ACCATAGCAG GACTGGAAGG CAATTCCAAC
    TCTCTGTGGA CCATCAGCCC TCCGAGCAGG ACAATGATGA TAGAAAGACT TATCCAGGAA
    CCTGACTTCA CAGCGGTCAT TTCTTGGTCC ATTCGAAGAA ATGTGAGTCT TGGAGCTAAG
    GCAGAGGTAA CAGGTGAAAA ATATACAATA TCACTGGCAG GTCAAACTCG GAGGGAACTT
    GCGGATATGA TGAATAGTTC ATCTCCCAAT AAGACAGTGG AACTGATAAA GATATACCCA
    TTGTGTTTAA AAGCACCAAG TGATGTTACT GCAAAACCCA TCCTTCAGCT TCTTAAGAAT
    CAAGAGTTTG TGAATATTAG CGTTTTACTA AGAAAAGAAA ACACATCAGA AGGGGTTCGA
    GAATGGTGGG TAGTGAATCA AAGCCATAAA GAGTTCATTA AGAAGGATGG CCTGGAACTA
    TGGGTTTTCA GTGACAAAGT CAGTCCTCCA AGTCTTGGAT TCTTGGCAGG CTATGGAATA
    ATGGGACTAT ATGCCTCTGT AGTTCTCGTA ATAGGAAAGT TTGTGCGTGA GTTTTTCAGC
    GGAATTTCGC ACTCCATCAT GTTTGAGGAG CTTCCCAACG TTGACCGTAT ACTGAAGCTG
    TGCACAGACA TTTTCCTTGT GAGAGAGACT GGAGAGTTGG AACTGGAGGA AGACCTGTAT
    GCCAAATTAA TATTTTTGTA CCGCTCCCCA GAAACTATGA TCAAATGGAC AAGAGAGAAA
    GATAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012820813.2
    CDS632..9100
    Translation

    Target ORF information:

    RefSeq Version XM_012965359.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis piezo-type mechanosensitive ion channel component 2 (piezo2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012965359.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGGCATCGG AAGTGGTGTG TGGGCTCATC TTCAGATTTC TTCTCCCACT ATGCCTGGTT 
    GTAGCGTGCA TGTTCCGGTA CAATGGAATA TCTTTTGTCT ACCTCATCTA CCTCTTGCTT
    ATACCTTTAT TTTCGGAGCC TACCAAAATA ACAATGCAAG GTCATACAGG ACGGTTATTA
    AAATCCTTAT GTGTTTCCAG TCTGTCATTT CTGCTGCTGC ACATCATCTT TCAAATTACC
    GTATACAGCC TCGAGGCCAG AAAACACATT GAACCACATT TTAACTGTTC AACATGGGAG
    AAAGCAATAC GACAAATTGG TTTTGAAAGT GTAAAAGGAG CAGATGCTGG TAATGGAATC
    CGCGTGTTTG CACCAGATAT CGGAATGTTT ATTGCAAGCT TGGCTACCCT GTTGGTTTGT
    AGAAAACTAG TTCAGAAAAC AGTTACTGAA GAGGACGCAC AATACAACCC CCAGTTTGTA
    AATGAAGAAA TGGCAGAAGA AGGAAAGGTG GACCTGGATG AAGCGTTAAT GTATGAGGAA
    GACTTTGATG GAGAAGAGGA TGAAATTGAG GCAGCTGAAG AAAGCACAAA ATTAAAAATT
    CTTCGCAGAA TTGCTTCATT TGCATCCGTA CTTAAAGAGT TTATTGGCAA CATTATAACC
    ACAGCTGGGA AAGTAGTTAT TACAATTTTA CTTGGTTTAA CAGGCATGAT GTTGCCGTCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT TTGGGGCTAT GCACATGGTG GTCCTGTTGC
    CGAGTATTTG ATCCGCTGAT ATTCAGCTGT CTATGTGTAT TAATGGCTAT ATTTAGTGCA
    GGGCACTTAA TTGGACTTTA TCTGTACCAG TTGCAGTTTT TTCACGAGAT TATTCCACCA
    AATGATTTCT ATGCCAGGTT GTTTGGTGTT ACTTCATTAG TTCAAACCGA CTGCTGTAAC
    ACTTGGAGAT TTTTTGCAAA CAACAGTCTA CCATGGAACC AGTATACCAA TCCTGTCATG
    CTTTTAGTAC TGTATTACAC GCTGGCAACT CTCATACGCC TTTGGCTACA GGAACCTATT
    AATGTAGAGG AAGAGAAGGA GGAAGACTGT AGCCCAGCAG CACTTACTGC AGAAAGAAGA
    CGTAGTTTGT GGTACTCTAG GCACTACACA ACGGATGAAA GACAACTTCT GTCCATGACT
    CAAGATGACT ACAAGCCATC TGATGTTTTA CTAGTAACTG TAAATGGGAC TCCCGTAGAT
    TACTGTTCAC TAAATTCATC ACTTCCGATA GAAAATGGTC CAACTAAAAT TGACATGTAT
    TCAACACCGC AATATAAATG GGACCATGAA GATGAAACTT CAGAAAAGAA AGAAGAGGAA
    GAGCAAGAAG AAGAACCCAA TGAAGAGGTT GCAGAAGAGA AAGAAAACAC TAAAGTTCAT
    GTAATGGTGG TGGCATTTCA GTTCATTATG AAGCAAAGCT ATATATGTGC ACTAATTTCA
    ATGATGGCCT GGAGCATCAC ATACCACAGC TGGTTGACTT TTGTTTTGTT AATCTGGTCT
    TGTGCTCTCT GGATGGTCCG AAATAGGAGA AAATATGCTA TGCTTAGTTC ACCTTTTATG
    GTTATGTATG GTAATTTACT TTTAACATTA CAATACATAT GGAGCATAGC CTTACAAAGT
    GATGAACTTC CAGAAGTTAT GGGTCTCCTT GAGAGAAAAA AGCCCGAAGA GCTTGTATCG
    AAGATCATTT TCACAGTTAC ATTTTGGCTT TTGCTCAGAC AACACCTTAC AGAGCAAAAG
    GCATTAAAGC AAAAAGAATT AGCATTATCT GAAATAAAAG TTGGAAGTGA AGACAAAGAA
    GGTGAAGTGC AAGAAGAAGA AGAAGAAGTG GAGGATGAAA ATGATATCAT GAAGGTCCTC
    GGAAAGCTAG TGCAGGCTAT GTTTGTCAAG TACTGGATTT ATGTCTGTGG CGGCATGTTT
    TTTTTTGTCA GCTTTGAAGG AAAAATAGTT ATGTACAAAA TTATCTACAT GATGCTCTTC
    CTATTCTGTG TAGCTCTGTA TCAGGTGCAC TATGAATGGT GGAGACGAAT TTTAAAATAT
    TTTTGGATGT CTGTGGTTGT TTACACCATG TTGGTGCTTG TTTTTATTTA CACTTACCAA
    TTTAAATCAT TTCCGGGCCT TTGGATGAAT ATGACAGGGC TGGATAAGGA AAAGTTAGCA
    GATCTTGGTT TAGAGACATT TAACTTGACT GACCTATTTA CTCGTATATT TATCCCAACC
    TCATTTTTGT TAGTGTGCAT TTTGCACCTA CATTACTTCC ATGACCGTTT TCTCCAGCTC
    ACTGACCTAA AGGCTGTAAC CAGCAAGCAG GACAGCACAA TTTACAGCCA TGCCAAAGTA
    AATGGTCGAG TTTACTTAAT AATAAATAGC ATCAAGCAAA AAATTCCAAT TCAGCAAAAT
    GAATTGGTGC ATCCAGAAGG AAGTTTGCCT GATATAACGA TGATGAACTT AACAACAGAA
    TCTGAAATGC TTGAGGAAAA GCGGTTGGAA ATTTATGACG AAAAAAAAAC TCATGACCAA
    GACTCAGATG ATGAATGGGA TAAAGGGGAT AGCAAAGAAC CAGGTCATTC TGATGATGAA
    GCTGAGGATG AGGAATCAGA AGAAGAAGAA ACAACAACAG AGTCCAGGAA CAAGTGGCAT
    CTAGTGATAG ATCGCTTAAC GGTTCTTTTC CTAAAGTTTC TGGAATATTT TCACAAGTTT
    CAGGTCTTTG TTTGGTGGCT TCTGGAGCTT CACATGATAA AGATTGTTTC TTCATACATC
    ATTTGGCTTA CTATAAAGGA GGTTTCACTG CTCAATTATG TATTTTTTAT TGCCTGGGCC
    TTCGCTTTGC CCTACTCACA ATTCCGGCCA TTGGCATCAA GTGTTTGCAC AGTCTGGACA
    TGTGTCATCA TCATATGTAA AATGTTGTAC CAGCTAGCCT CCATTGACCC TGCAACCTTT
    TCCAGCAACT GTTTAATGCC ACAGCTAAAC GAAACAACTG CACGTAACCA AACTGAGCTT
    AATTCTTCTG TGCTTTACAA TTCCTCTGTT GATCCTTCTA ATTGGGTTGG ACTAAAGAAG
    TCAACTCCAC TCCTTGCTTA TTTGCGGAAC AATTTGCTGA TGTTGGCCAT GTTAGCTTTT
    GAAGTAACCA TTTACCGGCA TCAGGAGTAT TACAGGTTGC GAAACAATCT CACCACACCT
    GCAACAAAAA CCATCTTTCA TGACATCACA AGACTTCACT TGGATGATGG ACTCATAAAT
    AGTGCCAAGT ATTTTGTGAA CTACTTTTTC TACAAGTTTG GATTGGAAAC ATGTTTCTTA
    CTGTCGGTAA ATGTAATCGG TCAGAGGATG GACTTTTTTG CCATGGTTCA TGCACTTTGG
    CTTTTTGGCG TATTATACCG ACGCAGAAGG AAAGCGATTG CAGAAACCTG GCACAGATAT
    TGCTGTTTTC TTGCTTGCAT TATGGCATTC CAGTATTTTA TATGTATTGG CATCCCCCCT
    GCTGCATGTA AAGACTATCC TTGGAGACAA CCTGGAGCCA ATTTTAATTC TAACATAATA
    AAGTGGTTAT ACTTTCCAGA CTTTATTGTG AGGCCTAGTC CTCTGTTTCT TGTCTATGAT
    TTTTTGCTTT TGCTTTGTGC GTCCCTTCAA AGAGAAGTGT TTGAGGAAGA AAATAAGGCA
    GCAGTTCGAA TCATGGCTGG TGACAATGTA GAAATTTGCA TGAACCTTGA TGCTGCATCT
    TTTAGCCAAC ATAATCCTGT ACCTGATTTC ATATACTGCA GATCTTATTT GGACATGGGT
    AAAGTTATAA TGTTTAGTTA CCTTTTCTGG TTTGTGCTCA CCATCATCTT CATCACGGGA
    ACTACACGGA TCAGCATATT CTGCATGGGC TACTTGGTAG CTTGCTTCTA TTTTCTATTA
    TTTGGTGGAG ATTTGCTGTT AAAACCTATA AGAATCATCC TACGCTATTG GGATTGGCTG
    ATTGCTTACA ATGTTTTTGT CATTACAATG AAAAATGTAT TATCAATAGG AGCATGTGGC
    TACATTGAAT ATTTAATCAA AGACAGTTGT TGGTTAATTC AGGCATTCAG TCTTTCTTGT
    ACAGTGAAAG GTTATTTTAT CCCCCAAAAC ACACCTGACT GTCAGTTGCC AAGCGGAGAG
    GCTGGAATCA TTTGGGACAG TATATGTTTT GCTTTCCTTT TATTGCAAAG AAGAGTGTTT
    ATGAGCTATT ACTTCTTACA TGTGGTTGCA GACATCAAAT CATCTCAGAT TCTAGCATCA
    AGAGGTGCTG AACTGTTCCA AGCTACAATT GTAAAAGCTG TAAAAGCAAG AATTGAAGAA
    GAAAAAAAAT CTATGGACCA GCTCAAGAGA CAGATGGACC ACATTAAAGC CAGGCAAGAA
    AAATATAAAA AAGGTAAAGA AAAAATGCTG AGTCTTACAC AGGATGGAGA AGGACAAGAA
    GTTCAAAAAC TAGATGATGA CGATGAAGAA GGAGACAAAG CAGACAAAGA GAAAGCCAAG
    GGCAAAAAAA AGCAGTGGTG GCGACCTTGG GTTGATCATG CTTCCATGGT GAAAAGCGGT
    GATTATTATT TGTTTGAGAC GGACAGTGAA GAAGAAGATG AGGATGACAA AAAAGAAGAA
    GAGCCTAGAA GAAAATCTGC CTTCCAGAGA GCTATTGGAA AGTTTGCATC AGCCATTATA
    GCCTTGCCAA AGTCTGTCAT TAAACTGCCT AAAACAATTC TTCAATATTT AATCAGAGCT
    GCAAAGTTTT TGTACCAAGC ATGGATTACT GATCCAAAAA CAGCCCTTAA GCAAAGAAGC
    ATGGAAGCCA AAGAAGAAAA GGCAAGGGAA ATGAGGCGAA AGGGCATTGC TGAAGGTGAT
    ATTATTGTAG AATGTGGGGA TAAAGAAGAA GAAAAAGAGC AAGGAAAAAA GAAATCAGAT
    GGCCCAGACA ATATCTTCAA AAGAATATTT AACATCCTGA AGTTTACTTG GGTCCTCTTC
    CTTGCTACAA TCGATAGTTT TACGGCTTGG CTAAACTCCA TTTCAAGAGA ACATATTGAT
    ATCTCCAAAG TTCTAAGCAT AGAACGTTGC ATGCTGACTA GAGAGATAAA AAAGGGCAAT
    GTGCCTACTC GAGAAAGCAT CCACATGTAT TACCAAAATC AAATAATGAT GAGCCTTTCT
    AGAGAGTCTG GACTTGACAG AATTGATGAC CAACCTGGTT CATCGTCCAG TCACCATAGC
    CATGACAAGA TGGCGAGTAA AAGGATGGAC AGTGCAGACT CAGCTGTATC TCATGATAGC
    ATATCAAGCT GCTGCACTGA AGCAACCATG CTATTCTCAA GGCAATCTAC ACTTGATGAT
    TTAGATGGAC CAGACTCTGT ACCAAAAACA AGTGAGCGTG CTCGACCTAG GCTTCGTAAA
    ATGTACAGCA TGGACATGTC TTCTTCATCT GCTGACAGTA TAGCTTCAAG TGAGCCAACT
    CAGTGCACCA TGGTCTACTC ACGGCAGGGC ACAACAGAAA CTATTGAGGA AGTGGAAGAT
    GAAGAGGAGG AAGAAAGCAA AAGTCCTTTA TCAAAGATAG AAGCCGAGGA AGAAGAATAT
    GACATGGATT TGGAAGGGAC AAGCTATACT CCGGACACAG AATTTCCCCT GTACGCCACT
    ACAGAGGGGG ATCTTCCTCC TTCGTACAGT AAAGCCGGCA GCTTCGAACA ACTTACATTT
    GTATCTCAGG ACTCGGCTGA CAATTTAATG GTTCTAAGCC CAGATGAAAG TCGTACTGAT
    AAGCTACATG ATCATATTCT TCCACCTCTT ACACATGAAC TGACTGCTAG CGAGCTGCTT
    CTAAACAAAA TGTTTTATGA TGAAGAGTTA GAAGACTCTG AAAAATTTTA TACTACTCAA
    CCACGCCCGC TGCAGCTTAT TTATGCCTTG TATAATACAT TGGTGGCCAG ATCAGAAATG
    GTGTGCTATT TTGTGATTAT TCTTAATCAC ATGATTTCAG CTTCTATGAT CACATTGGTA
    CTGCCCATCC TTATATTCCT TTGGGCTATG CTTTCTGTCC CAAGGCCCAG TAAGAGATTT
    TGGATGACAG CTATTGTATA TACAGAGGTG ACAATTGTTA TTAAATATTT TTTCCAATTT
    GGATTCTTCC CATGGAATAA AAATGTGGAT TATACAAAGG ACAAACCTTA CTTCCCACCT
    AACATAATGG GCATTGAGAA GAAAGAAGGT TATGTGCACT ATGACCTGGT GCAACTGCTT
    GCTCTGTTTT TCCATAGATC TATTTTAAAG TGCCACGGGC TTTGGGATGA AGATGATGTC
    ACTGAAAAAG CCACAGATAA AGACGACACG GATGGAGAGA TATCAGAGGC ATCAGGAAGA
    AGGGATTCCG TTGGCTCATT GAAATCTGTC AATCTAGCTG CGTCTGTGGA GTCAGTCCAT
    GTGCACTTCC CTGAGGAAAA GACCGCTATC AGGAGAAAAA GCACAAGCAG TGGATCGCAA
    ATTTCCCGAA GGTCAAGTTA TTCCTCGCAC AGGTCTAAAA GAGGCAGCAC CAGTACTCGG
    AACAGCAGTC AAAAAGGAAG CAGCATTCCC AGTATTAAAC TGAAATCTAG AAAGGAACTT
    ATACTGGAAA AACTCAGAGA ACAGCTCATT AAAGCAAAAG CATTTACGAT CAAGACGATA
    CTACAAATCT ATGTCCCGAT CAGACAGTTT TTTTACAATC TTATACATCC TGAATACAGT
    GCTGTGACTG ATGTCTATGT GCTGATGTTC CTGGCAGACA CTGTAGATTT CATCATTATT
    GTTTTTGGAT TTTGGGCGTT TGGAAAACAC TCCGCTGCAG CAGATATTAC ATCCTCTCTA
    TCAGAAGACC AAGTCCCAGA AGCATTTTTG GTAATGGTGC TTATCCAGTT TGGAACCATG
    GTTGTTGATC GTGCTTTGTA TCTTCGCAAA ACAGTCATGG GAAAAGTTAT TTTCCAAGTT
    ATCCTTGTAT TTGGAATCCA TTTTTGGATG TTCTTCATCT TGCCAGGGGT AACAGAAAGG
    AAATTTAGTC AGAACACTGT TGCCCAACTG TGGTATTTTG TCAAGTGCAT TTATTTTGGA
    TTGTCGGCAT ATCAGATACG TTGTGGATAT CCAACTCGGG TGCTTGGTAA CTTCCTTACT
    AAAAGCTACA ATTATGTCAA CCTGTTCCTT TTCCAAGGCT TTCGCCTCGT TCCATTTCTT
    ACGGAGCTGC GAGCTGTTAT GGACTGGGTA TGGACTGACA CCACTCTCAG TCTTTCCAGC
    TGGATCTGTG TAGAGGACAT TTATGCACAT ATATTTATAC TAAAATGCTG GCGGGAGTCA
    GAAAAGAGAT ATCCCCAGCC ACGAGGCCAA AAGAAGAAAA AGGTTGTGAA ATACGGAATG
    GGTGGAATGA TAATAGTTCT GCTGATTTGT ATCGTGTGGT TTCCTTTGCT CTTCATGTCC
    TTGATTAAAT CAGTTGCTGG GGTCACTAAC AAGCCCCTAG ATGTGTCCAT TACAATAACC
    CTTGGAGGTT ATCAGCCTAT TTTTACCATG AGTGCACAAC AAAATCAGCT GCAAGGCTTG
    AATGACAATG AATTTAAGCA TTTACATAAT ATATATAAAG GCAATACACC TGCCATGCAG
    TTTCTGGAAT CCTATATGCA GGAAGACATC ACCATAGCAG GACTGGAAGG CAATTCCAAC
    TCTCTGTGGA CCATCAGCCC TCCGAGCAGG ACAATGATGA TAGAAAGACT TATCCAGGAA
    CCTGACTTCA CAGCGGTCAT TTCTTGGTCC ATTCGAAGAA ATGTGAGTCT TGGAGCTAAG
    GCAGAGGTAA CAGGTGAAAA ATATACAATA TCACTGGCAG GTCAAACTCG GAGGGAACTT
    GCGGATATGA TGAATAGTTC ATCTCCCAAT AAGACAGTGG AACTGATAAA GATATACCCA
    TTGTGTTTAA AAGCACCAAG TGATGTTACT GCAAAACCCA TCCTTCAGCT TCTTAAGAAT
    CAAGAGTTTG TGAATATTAG CGTTTTACTA AGAAAAGAAA ACACATCAGA AGGGGTTCGA
    GAATGGTGGG TAGTGAATCA AAGCCATAAA GAGTTCATTA AGAAGGATGG CCTGGAACTA
    TGGGTTTTCA GTGACAAAGT CAGTCCTCCA AGTCTTGGAT TCTTGGCAGG CTATGGAATA
    ATGGGACTAT ATGCCTCTGT AGTTCTCGTA ATAGGAAAGT TTGTGCGTGA GTTTTTCAGC
    GGAATTTCGC ACTCCATCAT GTTTGAGGAG CTTCCCAACG TTGACCGTAT ACTGAAGCTG
    TGCACAGACA TTTTCCTTGT GAGAGAGACT GGAGAGTTGG AACTGGAGGA AGACCTGTAT
    GCCAAATTAA TATTTTTGTA CCGCTCCCCA GAAACTATGA TCAAATGGAC AAGAGAGAAA
    GATAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa60017
    Clone ID Related Accession (Same CDS sequence) XM_031903661.1
    Accession Version XM_031903661.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8436bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product piezo-type mechanosensitive ion channel component 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
    241
    301
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    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGGCATCGG AAGTGGTGTG TGGGCTCATC TTCAGATTTC TTCTCCCACT ATGCCTGGTT 
    GTAGCGTGCA TGTTCCGGTA CAATGGAATA TCTTTTGTCT ACCTCATCTA CCTCTTGCTT
    ATACCTTTAT TTTCGGAGCC TACCAAAATA ACAATGCAAG GTCATACAGG ACGGTTATTA
    AAATCCTTAT GTGTTTCCAG TCTGTCATTT CTGCTGCTGC ACATCATCTT TCAAATTACC
    GTATACAGCC TCGAGGCCAG AAAACACATT GAACCACATT TTAACTGTTC AACATGGGAG
    AAAGCAATAC GACAAATTGG TTTTGAAAGT GTAAAAGGAG CAGATGCTGG TAATGGAATC
    CGCGTGTTTG CACCAGATAT CGGAATGTTT ATTGCAAGCT TGGCTACCCT GTTGGTTTGT
    AGAAAACTAG TTCAGAAAAC AGTTACTGAA GAGGACGCAC AATACAACCC CCAGTTTGTA
    AATGAAGAAA TGGCAGAAGA AGGAAAGGTG GACCTGGATG AAGCGTTAAT GTATGAGGAA
    GACTTTGATG GAGAAGAGGA TGAAATTGAG GCAGCTGAAG AAAGCACAAA ATTAAAAATT
    CTTCGCAGAA TTGCTTCATT TGCATCCGTA CTTAAAGAGT TTATTGGCAA CATTATAACC
    ACAGCTGGGA AAGTAGTTAT TACAATTTTA CTTGGTTTAA CAGGCATGAT GTTGCCGTCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT TTGGGGCTAT GCACATGGTG GTCCTGTTGC
    CGAGTATTTG ATCCGCTGAT ATTCAGCTGT CTATGTGTAT TAATGGCTAT ATTTAGTGCA
    GGGCACTTAA TTGGACTTTA TCTGTACCAG TTGCAGTTTT TTCACGAGAT TATTCCACCA
    AATGATTTCT ATGCCAGGTT GTTTGGTGTT ACTTCATTAG TTCAAACCGA CTGCTGTAAC
    ACTTGGAGAT TTTTTGCAAA CAACAGTCTA CCATGGAACC AGTATACCAA TCCTGTCATG
    CTTTTAGTAC TGTATTACAC GCTGGCAACT CTCATACGCC TTTGGCTACA GGAACCTATT
    AATGTAGAGG AAGAGAAGGA GGAAGACTGT AGCCCAGCAG CACTTACTGC AGAAAGAAGA
    CGTAGTTTGT GGTACTCTAG GCACTACACA ACGGATGAAA GACAACTTCT GTCCATGACT
    CAAGATGACT ACAAGCCATC TGATGTTTTA CTAGTAACTG TAAATGGGAC TCCCGTAGAT
    TACTGTTCAC TAAATTCATC ACTTCCGATA GAAAATGGTC CAACTAAAAT TGACATGTAT
    TCAACACCGC AATATAAATG GGACCATGAA GATGAAACTT CAGAAAAGAA AGAAGAGGAA
    GAGCAAGAAG AAGAACCCAA TGAAGAGGTT GCAGAAGAGA AAGAAAACAC TAAAGTTCAT
    GTAATGGTGG TGGCATTTCA GTTCATTATG AAGCAAAGCT ATATATGTGC ACTAATTTCA
    ATGATGGCCT GGAGCATCAC ATACCACAGC TGGTTGACTT TTGTTTTGTT AATCTGGTCT
    TGTGCTCTCT GGATGGTCCG AAATAGGAGA AAATATGCTA TGCTTAGTTC ACCTTTTATG
    GTTATGTATG GTAATTTACT TTTAACATTA CAATACATAT GGAGCATAGC CTTACAAAGT
    GATGAACTTC CAGAAGTTAT GGGTCTCCTT GAGAGAAAAA AGCCCGAAGA GCTTGTATCG
    AAGATCATTT TCACAGTTAC ATTTTGGCTT TTGCTCAGAC AACACCTTAC AGAGCAAAAG
    GCATTAAAGC AAAAAGAATT AGCATTATCT GAAATAAAAG TTGGAAGTGA AGACAAAGAA
    GGTGAAGTGC AAGAAGAAGA AGAAGAAGTG GAGGATGAAA ATGATATCAT GAAGGTCCTC
    GGAAAGCTAG TGCAGGCTAT GTTTGTCAAG TACTGGATTT ATGTCTGTGG CGGCATGTTT
    TTTTTTGTCA GCTTTGAAGG AAAAATAGTT ATGTACAAAA TTATCTACAT GATGCTCTTC
    CTATTCTGTG TAGCTCTGTA TCAGGTGCAC TATGAATGGT GGAGACGAAT TTTAAAATAT
    TTTTGGATGT CTGTGGTTGT TTACACCATG TTGGTGCTTG TTTTTATTTA CACTTACCAA
    TTTAAATCAT TTCCGGGCCT TTGGATGAAT ATGACAGGGC TGGATAAGGA AAAGTTAGCA
    GATCTTGGTT TAGAGACATT TAACTTGACT GACCTATTTA CTCGTATATT TATCCCAACC
    TCATTTTTGT TAGTGTGCAT TTTGCACCTA CATTACTTCC ATGACCGTTT TCTCCAGCTC
    ACTGACCTAA AGGCTGTAAC CAGCAAGCAG GACAGCACAA TTTACAGCCA TGCCAAAGTA
    AATGGTCGAG TTTACTTAAT AATAAATAGA TTGGTGCATC CAGAAGGAAG TTTGCCTGAT
    ATAACGATGA TGAACTTAAC AACAGAATCT GAAATGCTTG AGGAAAAGCG GTTGGAAATT
    TATGACGAAA AAAAAACTCA TGACCAAGAC TCAGATGATG AATGGGATAA AGGGGATAGC
    AAAGAACCAG GTCATTCTGA TGATGAAGCT GAGGATGAGG AATCAGAAGA AGAAGAAACA
    ACAACAGAGT CCAGGAACAA GTGGCATCTA GTGATAGATC GCTTAACGGT TCTTTTCCTA
    AAGTTTCTGG AATATTTTCA CAAGTTTCAG GTCTTTGTTT GGTGGCTTCT GGAGCTTCAC
    ATGATAAAGA TTGTTTCTTC ATACATCATT TGGCTTACTA TAAAGGAGGT TTCACTGCTC
    AATTATGTAT TTTTTATTGC CTGGGCCTTC GCTTTGCCCT ACTCACAATT CCGGCCATTG
    GCATCAAGTG TTTGCACAGT CTGGACATGT GTCATCATCA TATGTAAAAT GTTGTACCAG
    CTAGCCTCCA TTGACCCTGC AACCTTTTCC AGCAACTGTT TAATGCCACA GCTAAACGAA
    ACAACTGCAC GTAACCAAAC TGAGCTTAAT TCTTCTGTGC TTTACAATTC CTCTGTTGAT
    CCTTCTAATT GGGTTGGACT AAAGAAGTCA ACTCCACTCC TTGCTTATTT GCGGAACAAT
    TTGCTGATGT TGGCCATGTT AGCTTTTGAA GTAACCATTT ACCGGCATCA GGAGTATTAC
    AGGTTGCGAA ACAATCTCAC CACACCTGCA ACAAAAACCA TCTTTCATGA CATCACAAGA
    CTTCACTTGG ATGATGGACT CATAAATAGT GCCAAGTATT TTGTGAACTA CTTTTTCTAC
    AAGTTTGGAT TGGAAACATG TTTCTTACTG TCGGTAAATG TAATCGGTCA GAGGATGGAC
    TTTTTTGCCA TGGTTCATGC ACTTTGGCTT TTTGGCGTAT TATACCGACG CAGAAGGAAA
    GCGATTGCAG AAACCTGGCA CAGATATTGC TGTTTTCTTG CTTGCATTAT GGCATTCCAG
    TATTTTATAT GTATTGGCAT CCCCCCTGCT GCATGTAAAG ACTATCCTTG GAGACAACCT
    GGAGCCAATT TTAATTCTAA CATAATAAAG TGGTTATACT TTCCAGACTT TATTGTGAGG
    CCTAGTCCTC TGTTTCTTGT CTATGATTTT TTGCTTTTGC TTTGTGCGTC CCTTCAAAGA
    GAAGTGTTTG AGGAAGAAAA TAAGGCAGCA GTTCGAATCA TGGCTGGTGA CAATGTAGAA
    ATTTGCATGA ACCTTGATGC TGCATCTTTT AGCCAACATA ATCCTGTACC TGATTTCATA
    TACTGCAGAT CTTATTTGGA CATGGGTAAA GTTATAATGT TTAGTTACCT TTTCTGGTTT
    GTGCTCACCA TCATCTTCAT CACGGGAACT ACACGGATCA GCATATTCTG CATGGGCTAC
    TTGGTAGCTT GCTTCTATTT TCTATTATTT GGTGGAGATT TGCTGTTAAA ACCTATAAGA
    ATCATCCTAC GCTATTGGGA TTGGCTGATT GCTTACAATG TTTTTGTCAT TACAATGAAA
    AATGTATTAT CAATAGGAGC ATGTGGCTAC ATTGAATATT TAATCAAAGA CAGTTGTTGG
    TTAATTCAGG CATTCAGTCT TTCTTGTACA GTGAAAGGTT ATTTTATCCC CCAAAACACA
    CCTGACTGTC AGTTGCCAAG CGGAGAGGCT GGAATCATTT GGGACAGTAT ATGTTTTGCT
    TTCCTTTTAT TGCAAAGAAG AGTGTTTATG AGCTATTACT TCTTACATGT GGTTGCAGAC
    ATCAAATCAT CTCAGATTCT AGCATCAAGA GGTGCTGAAC TGTTCCAAGC TACAATTGTA
    AAAGCTGTAA AAGCAAGAAT TGAAGAAGAA AAAAAATCTA TGGACCAGCT CAAGAGACAG
    ATGGACCACA TTAAAGCCAG GCAAGAAAAA TATAAAAAAG GTAAAGAAAA AATGCTGAGT
    CTTACACAGG ATGGAGAAGG ACAAGAAGTT CAAAAACTAG ATGATGACGA TGAAGAAGGA
    GACAAAGCAG ACAAAGAGAA AGCCAAGGGC AAAAAAAAGC AGTGGTGGCG ACCTTGGGTT
    GATCATGCTT CCATGGTGAA AAGCGGTGAT TATTATTTGT TTGAGACGGA CAGTGAAGAA
    GAAGATGAGG ATGACAAAAA AGAAGAAGAG CCTAGAAGAA AATCTGCCTT CCAGAGAGCT
    ATTGGAAAGT TTGCATCAGC CATTATAGCC TTGCCAAAGT CTGTCATTAA ACTGCCTAAA
    ACAATTCTTC AATATTTAAT CAGAGCTGCA AAGTTTTTGT ACCAAGCATG GATTACTGAT
    CCAAAAACAG CCCTTAAGCA AAGAAGCATG GAAGCCAAAG AAGAAAAGGC AAGGGAAATG
    AGGCGAAAGG GCATTGCTGA AGGTGATATT ATTGTAGAAT GTGGGGATAA AGAAGAAGAA
    AAAGAGCAAG GAAAAAAGAA ATCAGATGGC CCAGACAATA TCTTCAAAAG AATATTTAAC
    ATCCTGAAGT TTACTTGGGT CCTCTTCCTT GCTACAATCG ATAGTTTTAC GGCTTGGCTA
    AACTCCATTT CAAGAGAACA TATTGATATC TCCAAAGTTC TAAGCATAGA ACGTTGCATG
    CTGACTAGAG AGATAAAAAA GGGCAATGTG CCTACTCGAG AAAGCATCCA CATGTATTAC
    CAAAATCAAA TAATGATGAG CCTTTCTAGA GAGTCTGGAC TTGACAGAAT TGATGACCAA
    CCTGGTTCAT CGTCCAGTCA CCATAGCCAT GACAAGATGG CGAGTAAAAG GATGGACAGT
    GCAGACTCAG CTGTATCTCA TGATAGCATA TCAAGCTGCT GCACTGAAGC AACCATGCTA
    TTCTCAAGGC AATCTACACT TGATGATTTA GATGGACCAG ACTCTGTACC AAAAACAAGT
    GAGCGTGCTC GACCTAGGCT TCGTAAAATG TACAGCATGG ACATGTCTTC TTCATCTGCT
    GACAGTATAG CTTCAAGTGA GCCAACTCAG TGCACCATGG TCTACTCACG GCAGGGCACA
    ACAGAAACTA TTGAGGAAGT GGAAGATGAA GAGGAGGAAG AAAGCAAAAG TCCTTTATCA
    AAGATAGAAG CCGAGGAAGA AGAATATGAC ATGGATTTGG AAGGGACAAG CTATACTCCG
    GACACAGAAT TTCCCCTGTA CGCCACTACA GAGGGGGATC TTCCTCCTTC GTACAGTAAA
    GCCGGCAGCT TCGAACAACT TACATTTGTA TCTCAGGACT CGGCTGACAA TTTAATGGTT
    CTAAGCCCAG ATGAAAGTCG TACTGATAAG CTACATGATC ATATTCTTCC ACCTCTTACA
    CATGAACTGA CTGCTAGCGA GCTGCTTCTA AACAAAATGT TTTATGATGA AGAGTTAGAA
    GACTCTGAAA AATTTTATAC TACTCAACCA CGCCCGCTGC AGCTTATTTA TGCCTTGTAT
    AATACATTGG TGGCCAGATC AGAAATGGTG TGCTATTTTG TGATTATTCT TAATCACATG
    ATTTCAGCTT CTATGATCAC ATTGGTACTG CCCATCCTTA TATTCCTTTG GGCTATGCTT
    TCTGTCCCAA GGCCCAGTAA GAGATTTTGG ATGACAGCTA TTGTATATAC AGAGGTGACA
    ATTGTTATTA AATATTTTTT CCAATTTGGA TTCTTCCCAT GGAATAAAAA TGTGGATTAT
    ACAAAGGACA AACCTTACTT CCCACCTAAC ATAATGGGCA TTGAGAAGAA AGAAGGTTAT
    GTGCACTATG ACCTGGTGCA ACTGCTTGCT CTGTTTTTCC ATAGATCTAT TTTAAAGTGC
    CACGGGCTTT GGGATGAAGA TGATGTCACT GAAAAAGCCA CAGATAAAGA CGACACGGAT
    GGAGAGATAT CAGAGGCATC AGGAAGAAGG GATTCCGTTG GCTCATTGAA ATCTGTCAAT
    CTAGCTGCGT CTGTGGAGTC AGTCCATGTG CACTTCCCTG AGGAAAAGAC CGCTATCAGG
    AGAAAAAGCA CAAGCAGTGG ATCGCAAATT TCCCGAAGGT CAAGTTATTC CTCGCACAGG
    TCTAAAAGAG GCAGCACCAG TACTCGGAAC AGCAGTCAAA AAGGAAGCAG CATTCCCAGT
    ATTAAACTGA AATCTAGAAA GGAACTTATA CTGGAAAAAC TCAGAGAACA GCTCATTAAA
    GCAAAAGCAT TTACGATCAA GACGATACTA CAAATCTATG TCCCGATCAG ACAGTTTTTT
    TACAATCTTA TACATCCTGA ATACAGTGCT GTGACTGATG TCTATGTGCT GATGTTCCTG
    GCAGACACTG TAGATTTCAT CATTATTGTT TTTGGATTTT GGGCGTTTGG AAAACACTCC
    GCTGCAGCAG ATATTACATC CTCTCTATCA GAAGACCAAG TCCCAGAAGC ATTTTTGGTA
    ATGGTGCTTA TCCAGTTTGG AACCATGGTT GTTGATCGTG CTTTGTATCT TCGCAAAACA
    GTCATGGGAA AAGTTATTTT CCAAGTTATC CTTGTATTTG GAATCCATTT TTGGATGTTC
    TTCATCTTGC CAGGGGTAAC AGAAAGGAAA TTTAGTCAGA ACACTGTTGC CCAACTGTGG
    TATTTTGTCA AGTGCATTTA TTTTGGATTG TCGGCATATC AGATACGTTG TGGATATCCA
    ACTCGGGTGC TTGGTAACTT CCTTACTAAA AGCTACAATT ATGTCAACCT GTTCCTTTTC
    CAAGGCTTTC GCCTCGTTCC ATTTCTTACG GAGCTGCGAG CTGTTATGGA CTGGGTATGG
    ACTGACACCA CTCTCAGTCT TTCCAGCTGG ATCTGTGTAG AGGACATTTA TGCACATATA
    TTTATACTAA AATGCTGGCG GGAGTCAGAA AAGAGATATC CCCAGCCACG AGGCCAAAAG
    AAGAAAAAGG TTGTGAAATA CGGAATGGGT GGAATGATAA TAGTTCTGCT GATTTGTATC
    GTGTGGTTTC CTTTGCTCTT CATGTCCTTG ATTAAATCAG TTGCTGGGGT CACTAACAAG
    CCCCTAGATG TGTCCATTAC AATAACCCTT GGAGGTTATC AGCCTATTTT TACCATGAGT
    GCACAACAAA ATCAGCTGCA AGGCTTGAAT GACAATGAAT TTAAGCATTT ACATAATATA
    TATAAAGGCA ATACACCTGC CATGCAGTTT CTGGAATCCT ATATGCAGGA AGACATCACC
    ATAGCAGGAC TGGAAGGCAA TTCCAACTCT CTGTGGACCA TCAGCCCTCC GAGCAGGACA
    ATGATGATAG AAAGACTTAT CCAGGAACCT GACTTCACAG CGGTCATTTC TTGGTCCATT
    CGAAGAAATG TGAGTCTTGG AGCTAAGGCA GAGGTAACAG GTGAAAAATA TACAATATCA
    CTGGCAGGTC AAACTCGGAG GGAACTTGCG GATATGATGA ATAGTTCATC TCCCAATAAG
    ACAGTGGAAC TGATAAAGAT ATACCCATTG TGTTTAAAAG CACCAAGTGA TGTTACTGCA
    AAACCCATCC TTCAGCTTCT TAAGAATCAA GAGTTTGTGA ATATTAGCGT TTTACTAAGA
    AAAGAAAACA CATCAGAAGG GGTTCGAGAA TGGTGGGTAG TGAATCAAAG CCATAAAGAG
    TTCATTAAGA AGGATGGCCT GGAACTATGG GTTTTCAGTG ACAAAGTCAG TCCTCCAAGT
    CTTGGATTCT TGGCAGGCTA TGGAATAATG GGACTATATG CCTCTGTAGT TCTCGTAATA
    GGAAAGTTTG TGCGTGAGTT TTTCAGCGGA ATTTCGCACT CCATCATGTT TGAGGAGCTT
    CCCAACGTTG ACCGTATACT GAAGCTGTGC ACAGACATTT TCCTTGTGAG AGAGACTGGA
    GAGTTGGAAC TGGAGGAAGA CCTGTATGCC AAATTAATAT TTTTGTACCG CTCCCCAGAA
    ACTATGATCA AATGGACAAG AGAGAAAGAT AAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031759521.1
    CDS632..9067
    Translation

    Target ORF information:

    RefSeq Version XM_031903661.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis piezo-type mechanosensitive ion channel component 2 (piezo2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031903661.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGGCATCGG AAGTGGTGTG TGGGCTCATC TTCAGATTTC TTCTCCCACT ATGCCTGGTT 
    GTAGCGTGCA TGTTCCGGTA CAATGGAATA TCTTTTGTCT ACCTCATCTA CCTCTTGCTT
    ATACCTTTAT TTTCGGAGCC TACCAAAATA ACAATGCAAG GTCATACAGG ACGGTTATTA
    AAATCCTTAT GTGTTTCCAG TCTGTCATTT CTGCTGCTGC ACATCATCTT TCAAATTACC
    GTATACAGCC TCGAGGCCAG AAAACACATT GAACCACATT TTAACTGTTC AACATGGGAG
    AAAGCAATAC GACAAATTGG TTTTGAAAGT GTAAAAGGAG CAGATGCTGG TAATGGAATC
    CGCGTGTTTG CACCAGATAT CGGAATGTTT ATTGCAAGCT TGGCTACCCT GTTGGTTTGT
    AGAAAACTAG TTCAGAAAAC AGTTACTGAA GAGGACGCAC AATACAACCC CCAGTTTGTA
    AATGAAGAAA TGGCAGAAGA AGGAAAGGTG GACCTGGATG AAGCGTTAAT GTATGAGGAA
    GACTTTGATG GAGAAGAGGA TGAAATTGAG GCAGCTGAAG AAAGCACAAA ATTAAAAATT
    CTTCGCAGAA TTGCTTCATT TGCATCCGTA CTTAAAGAGT TTATTGGCAA CATTATAACC
    ACAGCTGGGA AAGTAGTTAT TACAATTTTA CTTGGTTTAA CAGGCATGAT GTTGCCGTCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT TTGGGGCTAT GCACATGGTG GTCCTGTTGC
    CGAGTATTTG ATCCGCTGAT ATTCAGCTGT CTATGTGTAT TAATGGCTAT ATTTAGTGCA
    GGGCACTTAA TTGGACTTTA TCTGTACCAG TTGCAGTTTT TTCACGAGAT TATTCCACCA
    AATGATTTCT ATGCCAGGTT GTTTGGTGTT ACTTCATTAG TTCAAACCGA CTGCTGTAAC
    ACTTGGAGAT TTTTTGCAAA CAACAGTCTA CCATGGAACC AGTATACCAA TCCTGTCATG
    CTTTTAGTAC TGTATTACAC GCTGGCAACT CTCATACGCC TTTGGCTACA GGAACCTATT
    AATGTAGAGG AAGAGAAGGA GGAAGACTGT AGCCCAGCAG CACTTACTGC AGAAAGAAGA
    CGTAGTTTGT GGTACTCTAG GCACTACACA ACGGATGAAA GACAACTTCT GTCCATGACT
    CAAGATGACT ACAAGCCATC TGATGTTTTA CTAGTAACTG TAAATGGGAC TCCCGTAGAT
    TACTGTTCAC TAAATTCATC ACTTCCGATA GAAAATGGTC CAACTAAAAT TGACATGTAT
    TCAACACCGC AATATAAATG GGACCATGAA GATGAAACTT CAGAAAAGAA AGAAGAGGAA
    GAGCAAGAAG AAGAACCCAA TGAAGAGGTT GCAGAAGAGA AAGAAAACAC TAAAGTTCAT
    GTAATGGTGG TGGCATTTCA GTTCATTATG AAGCAAAGCT ATATATGTGC ACTAATTTCA
    ATGATGGCCT GGAGCATCAC ATACCACAGC TGGTTGACTT TTGTTTTGTT AATCTGGTCT
    TGTGCTCTCT GGATGGTCCG AAATAGGAGA AAATATGCTA TGCTTAGTTC ACCTTTTATG
    GTTATGTATG GTAATTTACT TTTAACATTA CAATACATAT GGAGCATAGC CTTACAAAGT
    GATGAACTTC CAGAAGTTAT GGGTCTCCTT GAGAGAAAAA AGCCCGAAGA GCTTGTATCG
    AAGATCATTT TCACAGTTAC ATTTTGGCTT TTGCTCAGAC AACACCTTAC AGAGCAAAAG
    GCATTAAAGC AAAAAGAATT AGCATTATCT GAAATAAAAG TTGGAAGTGA AGACAAAGAA
    GGTGAAGTGC AAGAAGAAGA AGAAGAAGTG GAGGATGAAA ATGATATCAT GAAGGTCCTC
    GGAAAGCTAG TGCAGGCTAT GTTTGTCAAG TACTGGATTT ATGTCTGTGG CGGCATGTTT
    TTTTTTGTCA GCTTTGAAGG AAAAATAGTT ATGTACAAAA TTATCTACAT GATGCTCTTC
    CTATTCTGTG TAGCTCTGTA TCAGGTGCAC TATGAATGGT GGAGACGAAT TTTAAAATAT
    TTTTGGATGT CTGTGGTTGT TTACACCATG TTGGTGCTTG TTTTTATTTA CACTTACCAA
    TTTAAATCAT TTCCGGGCCT TTGGATGAAT ATGACAGGGC TGGATAAGGA AAAGTTAGCA
    GATCTTGGTT TAGAGACATT TAACTTGACT GACCTATTTA CTCGTATATT TATCCCAACC
    TCATTTTTGT TAGTGTGCAT TTTGCACCTA CATTACTTCC ATGACCGTTT TCTCCAGCTC
    ACTGACCTAA AGGCTGTAAC CAGCAAGCAG GACAGCACAA TTTACAGCCA TGCCAAAGTA
    AATGGTCGAG TTTACTTAAT AATAAATAGA TTGGTGCATC CAGAAGGAAG TTTGCCTGAT
    ATAACGATGA TGAACTTAAC AACAGAATCT GAAATGCTTG AGGAAAAGCG GTTGGAAATT
    TATGACGAAA AAAAAACTCA TGACCAAGAC TCAGATGATG AATGGGATAA AGGGGATAGC
    AAAGAACCAG GTCATTCTGA TGATGAAGCT GAGGATGAGG AATCAGAAGA AGAAGAAACA
    ACAACAGAGT CCAGGAACAA GTGGCATCTA GTGATAGATC GCTTAACGGT TCTTTTCCTA
    AAGTTTCTGG AATATTTTCA CAAGTTTCAG GTCTTTGTTT GGTGGCTTCT GGAGCTTCAC
    ATGATAAAGA TTGTTTCTTC ATACATCATT TGGCTTACTA TAAAGGAGGT TTCACTGCTC
    AATTATGTAT TTTTTATTGC CTGGGCCTTC GCTTTGCCCT ACTCACAATT CCGGCCATTG
    GCATCAAGTG TTTGCACAGT CTGGACATGT GTCATCATCA TATGTAAAAT GTTGTACCAG
    CTAGCCTCCA TTGACCCTGC AACCTTTTCC AGCAACTGTT TAATGCCACA GCTAAACGAA
    ACAACTGCAC GTAACCAAAC TGAGCTTAAT TCTTCTGTGC TTTACAATTC CTCTGTTGAT
    CCTTCTAATT GGGTTGGACT AAAGAAGTCA ACTCCACTCC TTGCTTATTT GCGGAACAAT
    TTGCTGATGT TGGCCATGTT AGCTTTTGAA GTAACCATTT ACCGGCATCA GGAGTATTAC
    AGGTTGCGAA ACAATCTCAC CACACCTGCA ACAAAAACCA TCTTTCATGA CATCACAAGA
    CTTCACTTGG ATGATGGACT CATAAATAGT GCCAAGTATT TTGTGAACTA CTTTTTCTAC
    AAGTTTGGAT TGGAAACATG TTTCTTACTG TCGGTAAATG TAATCGGTCA GAGGATGGAC
    TTTTTTGCCA TGGTTCATGC ACTTTGGCTT TTTGGCGTAT TATACCGACG CAGAAGGAAA
    GCGATTGCAG AAACCTGGCA CAGATATTGC TGTTTTCTTG CTTGCATTAT GGCATTCCAG
    TATTTTATAT GTATTGGCAT CCCCCCTGCT GCATGTAAAG ACTATCCTTG GAGACAACCT
    GGAGCCAATT TTAATTCTAA CATAATAAAG TGGTTATACT TTCCAGACTT TATTGTGAGG
    CCTAGTCCTC TGTTTCTTGT CTATGATTTT TTGCTTTTGC TTTGTGCGTC CCTTCAAAGA
    GAAGTGTTTG AGGAAGAAAA TAAGGCAGCA GTTCGAATCA TGGCTGGTGA CAATGTAGAA
    ATTTGCATGA ACCTTGATGC TGCATCTTTT AGCCAACATA ATCCTGTACC TGATTTCATA
    TACTGCAGAT CTTATTTGGA CATGGGTAAA GTTATAATGT TTAGTTACCT TTTCTGGTTT
    GTGCTCACCA TCATCTTCAT CACGGGAACT ACACGGATCA GCATATTCTG CATGGGCTAC
    TTGGTAGCTT GCTTCTATTT TCTATTATTT GGTGGAGATT TGCTGTTAAA ACCTATAAGA
    ATCATCCTAC GCTATTGGGA TTGGCTGATT GCTTACAATG TTTTTGTCAT TACAATGAAA
    AATGTATTAT CAATAGGAGC ATGTGGCTAC ATTGAATATT TAATCAAAGA CAGTTGTTGG
    TTAATTCAGG CATTCAGTCT TTCTTGTACA GTGAAAGGTT ATTTTATCCC CCAAAACACA
    CCTGACTGTC AGTTGCCAAG CGGAGAGGCT GGAATCATTT GGGACAGTAT ATGTTTTGCT
    TTCCTTTTAT TGCAAAGAAG AGTGTTTATG AGCTATTACT TCTTACATGT GGTTGCAGAC
    ATCAAATCAT CTCAGATTCT AGCATCAAGA GGTGCTGAAC TGTTCCAAGC TACAATTGTA
    AAAGCTGTAA AAGCAAGAAT TGAAGAAGAA AAAAAATCTA TGGACCAGCT CAAGAGACAG
    ATGGACCACA TTAAAGCCAG GCAAGAAAAA TATAAAAAAG GTAAAGAAAA AATGCTGAGT
    CTTACACAGG ATGGAGAAGG ACAAGAAGTT CAAAAACTAG ATGATGACGA TGAAGAAGGA
    GACAAAGCAG ACAAAGAGAA AGCCAAGGGC AAAAAAAAGC AGTGGTGGCG ACCTTGGGTT
    GATCATGCTT CCATGGTGAA AAGCGGTGAT TATTATTTGT TTGAGACGGA CAGTGAAGAA
    GAAGATGAGG ATGACAAAAA AGAAGAAGAG CCTAGAAGAA AATCTGCCTT CCAGAGAGCT
    ATTGGAAAGT TTGCATCAGC CATTATAGCC TTGCCAAAGT CTGTCATTAA ACTGCCTAAA
    ACAATTCTTC AATATTTAAT CAGAGCTGCA AAGTTTTTGT ACCAAGCATG GATTACTGAT
    CCAAAAACAG CCCTTAAGCA AAGAAGCATG GAAGCCAAAG AAGAAAAGGC AAGGGAAATG
    AGGCGAAAGG GCATTGCTGA AGGTGATATT ATTGTAGAAT GTGGGGATAA AGAAGAAGAA
    AAAGAGCAAG GAAAAAAGAA ATCAGATGGC CCAGACAATA TCTTCAAAAG AATATTTAAC
    ATCCTGAAGT TTACTTGGGT CCTCTTCCTT GCTACAATCG ATAGTTTTAC GGCTTGGCTA
    AACTCCATTT CAAGAGAACA TATTGATATC TCCAAAGTTC TAAGCATAGA ACGTTGCATG
    CTGACTAGAG AGATAAAAAA GGGCAATGTG CCTACTCGAG AAAGCATCCA CATGTATTAC
    CAAAATCAAA TAATGATGAG CCTTTCTAGA GAGTCTGGAC TTGACAGAAT TGATGACCAA
    CCTGGTTCAT CGTCCAGTCA CCATAGCCAT GACAAGATGG CGAGTAAAAG GATGGACAGT
    GCAGACTCAG CTGTATCTCA TGATAGCATA TCAAGCTGCT GCACTGAAGC AACCATGCTA
    TTCTCAAGGC AATCTACACT TGATGATTTA GATGGACCAG ACTCTGTACC AAAAACAAGT
    GAGCGTGCTC GACCTAGGCT TCGTAAAATG TACAGCATGG ACATGTCTTC TTCATCTGCT
    GACAGTATAG CTTCAAGTGA GCCAACTCAG TGCACCATGG TCTACTCACG GCAGGGCACA
    ACAGAAACTA TTGAGGAAGT GGAAGATGAA GAGGAGGAAG AAAGCAAAAG TCCTTTATCA
    AAGATAGAAG CCGAGGAAGA AGAATATGAC ATGGATTTGG AAGGGACAAG CTATACTCCG
    GACACAGAAT TTCCCCTGTA CGCCACTACA GAGGGGGATC TTCCTCCTTC GTACAGTAAA
    GCCGGCAGCT TCGAACAACT TACATTTGTA TCTCAGGACT CGGCTGACAA TTTAATGGTT
    CTAAGCCCAG ATGAAAGTCG TACTGATAAG CTACATGATC ATATTCTTCC ACCTCTTACA
    CATGAACTGA CTGCTAGCGA GCTGCTTCTA AACAAAATGT TTTATGATGA AGAGTTAGAA
    GACTCTGAAA AATTTTATAC TACTCAACCA CGCCCGCTGC AGCTTATTTA TGCCTTGTAT
    AATACATTGG TGGCCAGATC AGAAATGGTG TGCTATTTTG TGATTATTCT TAATCACATG
    ATTTCAGCTT CTATGATCAC ATTGGTACTG CCCATCCTTA TATTCCTTTG GGCTATGCTT
    TCTGTCCCAA GGCCCAGTAA GAGATTTTGG ATGACAGCTA TTGTATATAC AGAGGTGACA
    ATTGTTATTA AATATTTTTT CCAATTTGGA TTCTTCCCAT GGAATAAAAA TGTGGATTAT
    ACAAAGGACA AACCTTACTT CCCACCTAAC ATAATGGGCA TTGAGAAGAA AGAAGGTTAT
    GTGCACTATG ACCTGGTGCA ACTGCTTGCT CTGTTTTTCC ATAGATCTAT TTTAAAGTGC
    CACGGGCTTT GGGATGAAGA TGATGTCACT GAAAAAGCCA CAGATAAAGA CGACACGGAT
    GGAGAGATAT CAGAGGCATC AGGAAGAAGG GATTCCGTTG GCTCATTGAA ATCTGTCAAT
    CTAGCTGCGT CTGTGGAGTC AGTCCATGTG CACTTCCCTG AGGAAAAGAC CGCTATCAGG
    AGAAAAAGCA CAAGCAGTGG ATCGCAAATT TCCCGAAGGT CAAGTTATTC CTCGCACAGG
    TCTAAAAGAG GCAGCACCAG TACTCGGAAC AGCAGTCAAA AAGGAAGCAG CATTCCCAGT
    ATTAAACTGA AATCTAGAAA GGAACTTATA CTGGAAAAAC TCAGAGAACA GCTCATTAAA
    GCAAAAGCAT TTACGATCAA GACGATACTA CAAATCTATG TCCCGATCAG ACAGTTTTTT
    TACAATCTTA TACATCCTGA ATACAGTGCT GTGACTGATG TCTATGTGCT GATGTTCCTG
    GCAGACACTG TAGATTTCAT CATTATTGTT TTTGGATTTT GGGCGTTTGG AAAACACTCC
    GCTGCAGCAG ATATTACATC CTCTCTATCA GAAGACCAAG TCCCAGAAGC ATTTTTGGTA
    ATGGTGCTTA TCCAGTTTGG AACCATGGTT GTTGATCGTG CTTTGTATCT TCGCAAAACA
    GTCATGGGAA AAGTTATTTT CCAAGTTATC CTTGTATTTG GAATCCATTT TTGGATGTTC
    TTCATCTTGC CAGGGGTAAC AGAAAGGAAA TTTAGTCAGA ACACTGTTGC CCAACTGTGG
    TATTTTGTCA AGTGCATTTA TTTTGGATTG TCGGCATATC AGATACGTTG TGGATATCCA
    ACTCGGGTGC TTGGTAACTT CCTTACTAAA AGCTACAATT ATGTCAACCT GTTCCTTTTC
    CAAGGCTTTC GCCTCGTTCC ATTTCTTACG GAGCTGCGAG CTGTTATGGA CTGGGTATGG
    ACTGACACCA CTCTCAGTCT TTCCAGCTGG ATCTGTGTAG AGGACATTTA TGCACATATA
    TTTATACTAA AATGCTGGCG GGAGTCAGAA AAGAGATATC CCCAGCCACG AGGCCAAAAG
    AAGAAAAAGG TTGTGAAATA CGGAATGGGT GGAATGATAA TAGTTCTGCT GATTTGTATC
    GTGTGGTTTC CTTTGCTCTT CATGTCCTTG ATTAAATCAG TTGCTGGGGT CACTAACAAG
    CCCCTAGATG TGTCCATTAC AATAACCCTT GGAGGTTATC AGCCTATTTT TACCATGAGT
    GCACAACAAA ATCAGCTGCA AGGCTTGAAT GACAATGAAT TTAAGCATTT ACATAATATA
    TATAAAGGCA ATACACCTGC CATGCAGTTT CTGGAATCCT ATATGCAGGA AGACATCACC
    ATAGCAGGAC TGGAAGGCAA TTCCAACTCT CTGTGGACCA TCAGCCCTCC GAGCAGGACA
    ATGATGATAG AAAGACTTAT CCAGGAACCT GACTTCACAG CGGTCATTTC TTGGTCCATT
    CGAAGAAATG TGAGTCTTGG AGCTAAGGCA GAGGTAACAG GTGAAAAATA TACAATATCA
    CTGGCAGGTC AAACTCGGAG GGAACTTGCG GATATGATGA ATAGTTCATC TCCCAATAAG
    ACAGTGGAAC TGATAAAGAT ATACCCATTG TGTTTAAAAG CACCAAGTGA TGTTACTGCA
    AAACCCATCC TTCAGCTTCT TAAGAATCAA GAGTTTGTGA ATATTAGCGT TTTACTAAGA
    AAAGAAAACA CATCAGAAGG GGTTCGAGAA TGGTGGGTAG TGAATCAAAG CCATAAAGAG
    TTCATTAAGA AGGATGGCCT GGAACTATGG GTTTTCAGTG ACAAAGTCAG TCCTCCAAGT
    CTTGGATTCT TGGCAGGCTA TGGAATAATG GGACTATATG CCTCTGTAGT TCTCGTAATA
    GGAAAGTTTG TGCGTGAGTT TTTCAGCGGA ATTTCGCACT CCATCATGTT TGAGGAGCTT
    CCCAACGTTG ACCGTATACT GAAGCTGTGC ACAGACATTT TCCTTGTGAG AGAGACTGGA
    GAGTTGGAAC TGGAGGAAGA CCTGTATGCC AAATTAATAT TTTTGTACCG CTCCCCAGAA
    ACTATGATCA AATGGACAAG AGAGAAAGAT AAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa60018
    Clone ID Related Accession (Same CDS sequence) XM_002937476.5
    Accession Version XM_002937476.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8394bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product piezo-type mechanosensitive ion channel component 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002937476.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGGCATCGG AAGTGGTGTG TGGGCTCATC TTCAGATTTC TTCTCCCACT ATGCCTGGTT 
    GTAGCGTGCA TGTTCCGGTA CAATGGAATA TCTTTTGTCT ACCTCATCTA CCTCTTGCTT
    ATACCTTTAT TTTCGGAGCC TACCAAAATA ACAATGCAAG GTCATACAGG ACGGTTATTA
    AAATCCTTAT GTGTTTCCAG TCTGTCATTT CTGCTGCTGC ACATCATCTT TCAAATTACC
    GTATACAGCC TCGAGGCCAG AAAACACATT GAACCACATT TTAACTGTTC AACATGGGAG
    AAAGCAATAC GACAAATTGG TTTTGAAAGT GTAAAAGGAG CAGATGCTGG TAATGGAATC
    CGCGTGTTTG CACCAGATAT CGGAATGTTT ATTGCAAGCT TGGCTACCCT GTTGGTTTGT
    AGAAAACTAG TTCAGAAAAC AGTTACTGAA GAGGACGCAC AATACAACCC CCAGTTTGTA
    AATGAAGAAA TGGCAGAAGA AGGAAAGGTG GACCTGGATG AAGCGTTAAT GTATGAGGAA
    GACTTTGATG GAGAAGAGGA TGAAATTGAG GCAGCTGAAG AAAGCACAAA ATTAAAAATT
    CTTCGCAGAA TTGCTTCATT TGCATCCGTA CTTAAAGAGT TTATTGGCAA CATTATAACC
    ACAGCTGGGA AAGTAGTTAT TACAATTTTA CTTGGTTTAA CAGGCATGAT GTTGCCGTCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT TTGGGGCTAT GCACATGGTG GTCCTGTTGC
    CGAGTATTTG ATCCGCTGAT ATTCAGCTGT CTATGTGTAT TAATGGCTAT ATTTAGTGCA
    GGGCACTTAA TTGGACTTTA TCTGTACCAG TTGCAGTTTT TTCACGAGAT TATTCCACCA
    AATGATTTCT ATGCCAGGTT GTTTGGTGTT ACTTCATTAG TTCAAACCGA CTGCTGTAAC
    ACTTGGAGAT TTTTTGCAAA CAACAGTCTA CCATGGAACC AGTATACCAA TCCTGTCATG
    CTTTTAGTAC TGTATTACAC GCTGGCAACT CTCATACGCC TTTGGCTACA GGAACCTATT
    AATGTAGAGG AAGAGAAGGA GGAAGACTGT AGCCCAGCAG CACTTACTGC AGAAAGAAGA
    CGTAGTTTGT GGTACTCTAG GCACTACACA ACGGATGAAA GACAACTTCT GTCCATGACT
    CAAGATGACT ACAAGCCATC TGATGTTTTA CTAGTAACTG TAAATGGGAC TCCCGTAGAT
    TACTGTTCAC TAAATTCATC ACTTCCGATA GAAAATGGTC CAACTAAAAT TGACATGTAT
    TCAACACCGC AATATAAATG GGACCATGAA GATGAAACTT CAGAAAAGAA AGAAGAGGAA
    GAGCAAGAAG AAGAACCCAA TGAAGAGGTT GCAGAAGAGA AAGAAAACAC TAAAGTTCAT
    GTAATGGTGG TGGCATTTCA GTTCATTATG AAGCAAAGCT ATATATGTGC ACTAATTTCA
    ATGATGGCCT GGAGCATCAC ATACCACAGC TGGTTGACTT TTGTTTTGTT AATCTGGTCT
    TGTGCTCTCT GGATGGTCCG AAATAGGAGA AAATATGCTA TGCTTAGTTC ACCTTTTATG
    GTTATGTATG GTAATTTACT TTTAACATTA CAATACATAT GGAGCATAGC CTTACAAAGT
    GATGAACTTC CAGAAGTTAT GGGTCTCCTT GAGAGAAAAA AGCCCGAAGA GCTTGTATCG
    AAGATCATTT TCACAGTTAC ATTTTGGCTT TTGCTCAGAC AACACCTTAC AGAGCAAAAG
    GCATTAAAGC AAAAAGAATT AGCATTATCT GAAATAAAAG TTGGAAGTGA AGACAAAGAA
    GGTGAAGTGC AAGAAGAAGA AGAAGAAGTG GAGGATGAAA ATGATATCAT GAAGGTCCTC
    GGAAAGCTAG TGCAGGCTAT GTTTGTCAAG TACTGGATTT ATGTCTGTGG CGGCATGTTT
    TTTTTTGTCA GCTTTGAAGG AAAAATAGTT ATGTACAAAA TTATCTACAT GATGCTCTTC
    CTATTCTGTG TAGCTCTGTA TCAGGTGCAC TATGAATGGT GGAGACGAAT TTTAAAATAT
    TTTTGGATGT CTGTGGTTGT TTACACCATG TTGGTGCTTG TTTTTATTTA CACTTACCAA
    TTTAAATCAT TTCCGGGCCT TTGGATGAAT ATGACAGGGC TGGATAAGGA AAAGTTAGCA
    GATCTTGGTT TAGAGACATT TAACTTGACT GACCTATTTA CTCGTATATT TATCCCAACC
    TCATTTTTGT TAGTGTGCAT TTTGCACCTA CATTACTTCC ATGACCGTTT TCTCCAGCTC
    ACTGACCTAA AGGCTGTAAC CAGCAAGCAG GACAGCACAA TTTACAGATT GGTGCATCCA
    GAAGGAAGTT TGCCTGATAT AACGATGATG AACTTAACAA CAGAATCTGA AATGCTTGAG
    GAAAAGCGGT TGGAAATTTA TGACGAAAAA AAAACTCATG ACCAAGACTC AGATGATGAA
    TGGGATAAAG GGGATAGCAA AGAACCAGGT CATTCTGATG ATGAAGCTGA GGATGAGGAA
    TCAGAAGAAG AAGAAACAAC AACAGAGTCC AGGAACAAGT GGCATCTAGT GATAGATCGC
    TTAACGGTTC TTTTCCTAAA GTTTCTGGAA TATTTTCACA AGTTTCAGGT CTTTGTTTGG
    TGGCTTCTGG AGCTTCACAT GATAAAGATT GTTTCTTCAT ACATCATTTG GCTTACTATA
    AAGGAGGTTT CACTGCTCAA TTATGTATTT TTTATTGCCT GGGCCTTCGC TTTGCCCTAC
    TCACAATTCC GGCCATTGGC ATCAAGTGTT TGCACAGTCT GGACATGTGT CATCATCATA
    TGTAAAATGT TGTACCAGCT AGCCTCCATT GACCCTGCAA CCTTTTCCAG CAACTGTTTA
    ATGCCACAGC TAAACGAAAC AACTGCACGT AACCAAACTG AGCTTAATTC TTCTGTGCTT
    TACAATTCCT CTGTTGATCC TTCTAATTGG GTTGGACTAA AGAAGTCAAC TCCACTCCTT
    GCTTATTTGC GGAACAATTT GCTGATGTTG GCCATGTTAG CTTTTGAAGT AACCATTTAC
    CGGCATCAGG AGTATTACAG GTTGCGAAAC AATCTCACCA CACCTGCAAC AAAAACCATC
    TTTCATGACA TCACAAGACT TCACTTGGAT GATGGACTCA TAAATAGTGC CAAGTATTTT
    GTGAACTACT TTTTCTACAA GTTTGGATTG GAAACATGTT TCTTACTGTC GGTAAATGTA
    ATCGGTCAGA GGATGGACTT TTTTGCCATG GTTCATGCAC TTTGGCTTTT TGGCGTATTA
    TACCGACGCA GAAGGAAAGC GATTGCAGAA ACCTGGCACA GATATTGCTG TTTTCTTGCT
    TGCATTATGG CATTCCAGTA TTTTATATGT ATTGGCATCC CCCCTGCTGC ATGTAAAGAC
    TATCCTTGGA GACAACCTGG AGCCAATTTT AATTCTAACA TAATAAAGTG GTTATACTTT
    CCAGACTTTA TTGTGAGGCC TAGTCCTCTG TTTCTTGTCT ATGATTTTTT GCTTTTGCTT
    TGTGCGTCCC TTCAAAGAGA AGTGTTTGAG GAAGAAAATA AGGCAGCAGT TCGAATCATG
    GCTGGTGACA ATGTAGAAAT TTGCATGAAC CTTGATGCTG CATCTTTTAG CCAACATAAT
    CCTGTACCTG ATTTCATATA CTGCAGATCT TATTTGGACA TGGGTAAAGT TATAATGTTT
    AGTTACCTTT TCTGGTTTGT GCTCACCATC ATCTTCATCA CGGGAACTAC ACGGATCAGC
    ATATTCTGCA TGGGCTACTT GGTAGCTTGC TTCTATTTTC TATTATTTGG TGGAGATTTG
    CTGTTAAAAC CTATAAGAAT CATCCTACGC TATTGGGATT GGCTGATTGC TTACAATGTT
    TTTGTCATTA CAATGAAAAA TGTATTATCA ATAGGAGCAT GTGGCTACAT TGAATATTTA
    ATCAAAGACA GTTGTTGGTT AATTCAGGCA TTCAGTCTTT CTTGTACAGT GAAAGGTTAT
    TTTATCCCCC AAAACACACC TGACTGTCAG TTGCCAAGCG GAGAGGCTGG AATCATTTGG
    GACAGTATAT GTTTTGCTTT CCTTTTATTG CAAAGAAGAG TGTTTATGAG CTATTACTTC
    TTACATGTGG TTGCAGACAT CAAATCATCT CAGATTCTAG CATCAAGAGG TGCTGAACTG
    TTCCAAGCTA CAATTGTAAA AGCTGTAAAA GCAAGAATTG AAGAAGAAAA AAAATCTATG
    GACCAGCTCA AGAGACAGAT GGACCACATT AAAGCCAGGC AAGAAAAATA TAAAAAAGGT
    AAAGAAAAAA TGCTGAGTCT TACACAGGAT GGAGAAGGAC AAGAAGTTCA AAAACTAGAT
    GATGACGATG AAGAAGGAGA CAAAGCAGAC AAAGAGAAAG CCAAGGGCAA AAAAAAGCAG
    TGGTGGCGAC CTTGGGTTGA TCATGCTTCC ATGGTGAAAA GCGGTGATTA TTATTTGTTT
    GAGACGGACA GTGAAGAAGA AGATGAGGAT GACAAAAAAG AAGAAGAGCC TAGAAGAAAA
    TCTGCCTTCC AGAGAGCTAT TGGAAAGTTT GCATCAGCCA TTATAGCCTT GCCAAAGTCT
    GTCATTAAAC TGCCTAAAAC AATTCTTCAA TATTTAATCA GAGCTGCAAA GTTTTTGTAC
    CAAGCATGGA TTACTGATCC AAAAACAGCC CTTAAGCAAA GAAGCATGGA AGCCAAAGAA
    GAAAAGGCAA GGGAAATGAG GCGAAAGGGC ATTGCTGAAG GTGATATTAT TGTAGAATGT
    GGGGATAAAG AAGAAGAAAA AGAGCAAGGA AAAAAGAAAT CAGATGGCCC AGACAATATC
    TTCAAAAGAA TATTTAACAT CCTGAAGTTT ACTTGGGTCC TCTTCCTTGC TACAATCGAT
    AGTTTTACGG CTTGGCTAAA CTCCATTTCA AGAGAACATA TTGATATCTC CAAAGTTCTA
    AGCATAGAAC GTTGCATGCT GACTAGAGAG ATAAAAAAGG GCAATGTGCC TACTCGAGAA
    AGCATCCACA TGTATTACCA AAATCAAATA ATGATGAGCC TTTCTAGAGA GTCTGGACTT
    GACAGAATTG ATGACCAACC TGGTTCATCG TCCAGTCACC ATAGCCATGA CAAGATGGCG
    AGTAAAAGGA TGGACAGTGC AGACTCAGCT GTATCTCATG ATAGCATATC AAGCTGCTGC
    ACTGAAGCAA CCATGCTATT CTCAAGGCAA TCTACACTTG ATGATTTAGA TGGACCAGAC
    TCTGTACCAA AAACAAGTGA GCGTGCTCGA CCTAGGCTTC GTAAAATGTA CAGCATGGAC
    ATGTCTTCTT CATCTGCTGA CAGTATAGCT TCAAGTGAGC CAACTCAGTG CACCATGGTC
    TACTCACGGC AGGGCACAAC AGAAACTATT GAGGAAGTGG AAGATGAAGA GGAGGAAGAA
    AGCAAAAGTC CTTTATCAAA GATAGAAGCC GAGGAAGAAG AATATGACAT GGATTTGGAA
    GGGACAAGCT ATACTCCGGA CACAGAATTT CCCCTGTACG CCACTACAGA GGGGGATCTT
    CCTCCTTCGT ACAGTAAAGC CGGCAGCTTC GAACAACTTA CATTTGTATC TCAGGACTCG
    GCTGACAATT TAATGGTTCT AAGCCCAGAT GAAAGTCGTA CTGATAAGCT ACATGATCAT
    ATTCTTCCAC CTCTTACACA TGAACTGACT GCTAGCGAGC TGCTTCTAAA CAAAATGTTT
    TATGATGAAG AGTTAGAAGA CTCTGAAAAA TTTTATACTA CTCAACCACG CCCGCTGCAG
    CTTATTTATG CCTTGTATAA TACATTGGTG GCCAGATCAG AAATGGTGTG CTATTTTGTG
    ATTATTCTTA ATCACATGAT TTCAGCTTCT ATGATCACAT TGGTACTGCC CATCCTTATA
    TTCCTTTGGG CTATGCTTTC TGTCCCAAGG CCCAGTAAGA GATTTTGGAT GACAGCTATT
    GTATATACAG AGGTGACAAT TGTTATTAAA TATTTTTTCC AATTTGGATT CTTCCCATGG
    AATAAAAATG TGGATTATAC AAAGGACAAA CCTTACTTCC CACCTAACAT AATGGGCATT
    GAGAAGAAAG AAGGTTATGT GCACTATGAC CTGGTGCAAC TGCTTGCTCT GTTTTTCCAT
    AGATCTATTT TAAAGTGCCA CGGGCTTTGG GATGAAGATG ATGTCACTGA AAAAGCCACA
    GATAAAGACG ACACGGATGG AGAGATATCA GAGGCATCAG GAAGAAGGGA TTCCGTTGGC
    TCATTGAAAT CTGTCAATCT AGCTGCGTCT GTGGAGTCAG TCCATGTGCA CTTCCCTGAG
    GAAAAGACCG CTATCAGGAG AAAAAGCACA AGCAGTGGAT CGCAAATTTC CCGAAGGTCA
    AGTTATTCCT CGCACAGGTC TAAAAGAGGC AGCACCAGTA CTCGGAACAG CAGTCAAAAA
    GGAAGCAGCA TTCCCAGTAT TAAACTGAAA TCTAGAAAGG AACTTATACT GGAAAAACTC
    AGAGAACAGC TCATTAAAGC AAAAGCATTT ACGATCAAGA CGATACTACA AATCTATGTC
    CCGATCAGAC AGTTTTTTTA CAATCTTATA CATCCTGAAT ACAGTGCTGT GACTGATGTC
    TATGTGCTGA TGTTCCTGGC AGACACTGTA GATTTCATCA TTATTGTTTT TGGATTTTGG
    GCGTTTGGAA AACACTCCGC TGCAGCAGAT ATTACATCCT CTCTATCAGA AGACCAAGTC
    CCAGAAGCAT TTTTGGTAAT GGTGCTTATC CAGTTTGGAA CCATGGTTGT TGATCGTGCT
    TTGTATCTTC GCAAAACAGT CATGGGAAAA GTTATTTTCC AAGTTATCCT TGTATTTGGA
    ATCCATTTTT GGATGTTCTT CATCTTGCCA GGGGTAACAG AAAGGAAATT TAGTCAGAAC
    ACTGTTGCCC AACTGTGGTA TTTTGTCAAG TGCATTTATT TTGGATTGTC GGCATATCAG
    ATACGTTGTG GATATCCAAC TCGGGTGCTT GGTAACTTCC TTACTAAAAG CTACAATTAT
    GTCAACCTGT TCCTTTTCCA AGGCTTTCGC CTCGTTCCAT TTCTTACGGA GCTGCGAGCT
    GTTATGGACT GGGTATGGAC TGACACCACT CTCAGTCTTT CCAGCTGGAT CTGTGTAGAG
    GACATTTATG CACATATATT TATACTAAAA TGCTGGCGGG AGTCAGAAAA GAGATATCCC
    CAGCCACGAG GCCAAAAGAA GAAAAAGGTT GTGAAATACG GAATGGGTGG AATGATAATA
    GTTCTGCTGA TTTGTATCGT GTGGTTTCCT TTGCTCTTCA TGTCCTTGAT TAAATCAGTT
    GCTGGGGTCA CTAACAAGCC CCTAGATGTG TCCATTACAA TAACCCTTGG AGGTTATCAG
    CCTATTTTTA CCATGAGTGC ACAACAAAAT CAGCTGCAAG GCTTGAATGA CAATGAATTT
    AAGCATTTAC ATAATATATA TAAAGGCAAT ACACCTGCCA TGCAGTTTCT GGAATCCTAT
    ATGCAGGAAG ACATCACCAT AGCAGGACTG GAAGGCAATT CCAACTCTCT GTGGACCATC
    AGCCCTCCGA GCAGGACAAT GATGATAGAA AGACTTATCC AGGAACCTGA CTTCACAGCG
    GTCATTTCTT GGTCCATTCG AAGAAATGTG AGTCTTGGAG CTAAGGCAGA GGTAACAGGT
    GAAAAATATA CAATATCACT GGCAGGTCAA ACTCGGAGGG AACTTGCGGA TATGATGAAT
    AGTTCATCTC CCAATAAGAC AGTGGAACTG ATAAAGATAT ACCCATTGTG TTTAAAAGCA
    CCAAGTGATG TTACTGCAAA ACCCATCCTT CAGCTTCTTA AGAATCAAGA GTTTGTGAAT
    ATTAGCGTTT TACTAAGAAA AGAAAACACA TCAGAAGGGG TTCGAGAATG GTGGGTAGTG
    AATCAAAGCC ATAAAGAGTT CATTAAGAAG GATGGCCTGG AACTATGGGT TTTCAGTGAC
    AAAGTCAGTC CTCCAAGTCT TGGATTCTTG GCAGGCTATG GAATAATGGG ACTATATGCC
    TCTGTAGTTC TCGTAATAGG AAAGTTTGTG CGTGAGTTTT TCAGCGGAAT TTCGCACTCC
    ATCATGTTTG AGGAGCTTCC CAACGTTGAC CGTATACTGA AGCTGTGCAC AGACATTTTC
    CTTGTGAGAG AGACTGGAGA GTTGGAACTG GAGGAAGACC TGTATGCCAA ATTAATATTT
    TTGTACCGCT CCCCAGAAAC TATGATCAAA TGGACAAGAG AGAAAGATAA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002937522.3
    CDS632..9025
    Translation

    Target ORF information:

    RefSeq Version XM_002937476.5
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis piezo-type mechanosensitive ion channel component 2 (piezo2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002937476.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGGCATCGG AAGTGGTGTG TGGGCTCATC TTCAGATTTC TTCTCCCACT ATGCCTGGTT 
    GTAGCGTGCA TGTTCCGGTA CAATGGAATA TCTTTTGTCT ACCTCATCTA CCTCTTGCTT
    ATACCTTTAT TTTCGGAGCC TACCAAAATA ACAATGCAAG GTCATACAGG ACGGTTATTA
    AAATCCTTAT GTGTTTCCAG TCTGTCATTT CTGCTGCTGC ACATCATCTT TCAAATTACC
    GTATACAGCC TCGAGGCCAG AAAACACATT GAACCACATT TTAACTGTTC AACATGGGAG
    AAAGCAATAC GACAAATTGG TTTTGAAAGT GTAAAAGGAG CAGATGCTGG TAATGGAATC
    CGCGTGTTTG CACCAGATAT CGGAATGTTT ATTGCAAGCT TGGCTACCCT GTTGGTTTGT
    AGAAAACTAG TTCAGAAAAC AGTTACTGAA GAGGACGCAC AATACAACCC CCAGTTTGTA
    AATGAAGAAA TGGCAGAAGA AGGAAAGGTG GACCTGGATG AAGCGTTAAT GTATGAGGAA
    GACTTTGATG GAGAAGAGGA TGAAATTGAG GCAGCTGAAG AAAGCACAAA ATTAAAAATT
    CTTCGCAGAA TTGCTTCATT TGCATCCGTA CTTAAAGAGT TTATTGGCAA CATTATAACC
    ACAGCTGGGA AAGTAGTTAT TACAATTTTA CTTGGTTTAA CAGGCATGAT GTTGCCGTCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT TTGGGGCTAT GCACATGGTG GTCCTGTTGC
    CGAGTATTTG ATCCGCTGAT ATTCAGCTGT CTATGTGTAT TAATGGCTAT ATTTAGTGCA
    GGGCACTTAA TTGGACTTTA TCTGTACCAG TTGCAGTTTT TTCACGAGAT TATTCCACCA
    AATGATTTCT ATGCCAGGTT GTTTGGTGTT ACTTCATTAG TTCAAACCGA CTGCTGTAAC
    ACTTGGAGAT TTTTTGCAAA CAACAGTCTA CCATGGAACC AGTATACCAA TCCTGTCATG
    CTTTTAGTAC TGTATTACAC GCTGGCAACT CTCATACGCC TTTGGCTACA GGAACCTATT
    AATGTAGAGG AAGAGAAGGA GGAAGACTGT AGCCCAGCAG CACTTACTGC AGAAAGAAGA
    CGTAGTTTGT GGTACTCTAG GCACTACACA ACGGATGAAA GACAACTTCT GTCCATGACT
    CAAGATGACT ACAAGCCATC TGATGTTTTA CTAGTAACTG TAAATGGGAC TCCCGTAGAT
    TACTGTTCAC TAAATTCATC ACTTCCGATA GAAAATGGTC CAACTAAAAT TGACATGTAT
    TCAACACCGC AATATAAATG GGACCATGAA GATGAAACTT CAGAAAAGAA AGAAGAGGAA
    GAGCAAGAAG AAGAACCCAA TGAAGAGGTT GCAGAAGAGA AAGAAAACAC TAAAGTTCAT
    GTAATGGTGG TGGCATTTCA GTTCATTATG AAGCAAAGCT ATATATGTGC ACTAATTTCA
    ATGATGGCCT GGAGCATCAC ATACCACAGC TGGTTGACTT TTGTTTTGTT AATCTGGTCT
    TGTGCTCTCT GGATGGTCCG AAATAGGAGA AAATATGCTA TGCTTAGTTC ACCTTTTATG
    GTTATGTATG GTAATTTACT TTTAACATTA CAATACATAT GGAGCATAGC CTTACAAAGT
    GATGAACTTC CAGAAGTTAT GGGTCTCCTT GAGAGAAAAA AGCCCGAAGA GCTTGTATCG
    AAGATCATTT TCACAGTTAC ATTTTGGCTT TTGCTCAGAC AACACCTTAC AGAGCAAAAG
    GCATTAAAGC AAAAAGAATT AGCATTATCT GAAATAAAAG TTGGAAGTGA AGACAAAGAA
    GGTGAAGTGC AAGAAGAAGA AGAAGAAGTG GAGGATGAAA ATGATATCAT GAAGGTCCTC
    GGAAAGCTAG TGCAGGCTAT GTTTGTCAAG TACTGGATTT ATGTCTGTGG CGGCATGTTT
    TTTTTTGTCA GCTTTGAAGG AAAAATAGTT ATGTACAAAA TTATCTACAT GATGCTCTTC
    CTATTCTGTG TAGCTCTGTA TCAGGTGCAC TATGAATGGT GGAGACGAAT TTTAAAATAT
    TTTTGGATGT CTGTGGTTGT TTACACCATG TTGGTGCTTG TTTTTATTTA CACTTACCAA
    TTTAAATCAT TTCCGGGCCT TTGGATGAAT ATGACAGGGC TGGATAAGGA AAAGTTAGCA
    GATCTTGGTT TAGAGACATT TAACTTGACT GACCTATTTA CTCGTATATT TATCCCAACC
    TCATTTTTGT TAGTGTGCAT TTTGCACCTA CATTACTTCC ATGACCGTTT TCTCCAGCTC
    ACTGACCTAA AGGCTGTAAC CAGCAAGCAG GACAGCACAA TTTACAGATT GGTGCATCCA
    GAAGGAAGTT TGCCTGATAT AACGATGATG AACTTAACAA CAGAATCTGA AATGCTTGAG
    GAAAAGCGGT TGGAAATTTA TGACGAAAAA AAAACTCATG ACCAAGACTC AGATGATGAA
    TGGGATAAAG GGGATAGCAA AGAACCAGGT CATTCTGATG ATGAAGCTGA GGATGAGGAA
    TCAGAAGAAG AAGAAACAAC AACAGAGTCC AGGAACAAGT GGCATCTAGT GATAGATCGC
    TTAACGGTTC TTTTCCTAAA GTTTCTGGAA TATTTTCACA AGTTTCAGGT CTTTGTTTGG
    TGGCTTCTGG AGCTTCACAT GATAAAGATT GTTTCTTCAT ACATCATTTG GCTTACTATA
    AAGGAGGTTT CACTGCTCAA TTATGTATTT TTTATTGCCT GGGCCTTCGC TTTGCCCTAC
    TCACAATTCC GGCCATTGGC ATCAAGTGTT TGCACAGTCT GGACATGTGT CATCATCATA
    TGTAAAATGT TGTACCAGCT AGCCTCCATT GACCCTGCAA CCTTTTCCAG CAACTGTTTA
    ATGCCACAGC TAAACGAAAC AACTGCACGT AACCAAACTG AGCTTAATTC TTCTGTGCTT
    TACAATTCCT CTGTTGATCC TTCTAATTGG GTTGGACTAA AGAAGTCAAC TCCACTCCTT
    GCTTATTTGC GGAACAATTT GCTGATGTTG GCCATGTTAG CTTTTGAAGT AACCATTTAC
    CGGCATCAGG AGTATTACAG GTTGCGAAAC AATCTCACCA CACCTGCAAC AAAAACCATC
    TTTCATGACA TCACAAGACT TCACTTGGAT GATGGACTCA TAAATAGTGC CAAGTATTTT
    GTGAACTACT TTTTCTACAA GTTTGGATTG GAAACATGTT TCTTACTGTC GGTAAATGTA
    ATCGGTCAGA GGATGGACTT TTTTGCCATG GTTCATGCAC TTTGGCTTTT TGGCGTATTA
    TACCGACGCA GAAGGAAAGC GATTGCAGAA ACCTGGCACA GATATTGCTG TTTTCTTGCT
    TGCATTATGG CATTCCAGTA TTTTATATGT ATTGGCATCC CCCCTGCTGC ATGTAAAGAC
    TATCCTTGGA GACAACCTGG AGCCAATTTT AATTCTAACA TAATAAAGTG GTTATACTTT
    CCAGACTTTA TTGTGAGGCC TAGTCCTCTG TTTCTTGTCT ATGATTTTTT GCTTTTGCTT
    TGTGCGTCCC TTCAAAGAGA AGTGTTTGAG GAAGAAAATA AGGCAGCAGT TCGAATCATG
    GCTGGTGACA ATGTAGAAAT TTGCATGAAC CTTGATGCTG CATCTTTTAG CCAACATAAT
    CCTGTACCTG ATTTCATATA CTGCAGATCT TATTTGGACA TGGGTAAAGT TATAATGTTT
    AGTTACCTTT TCTGGTTTGT GCTCACCATC ATCTTCATCA CGGGAACTAC ACGGATCAGC
    ATATTCTGCA TGGGCTACTT GGTAGCTTGC TTCTATTTTC TATTATTTGG TGGAGATTTG
    CTGTTAAAAC CTATAAGAAT CATCCTACGC TATTGGGATT GGCTGATTGC TTACAATGTT
    TTTGTCATTA CAATGAAAAA TGTATTATCA ATAGGAGCAT GTGGCTACAT TGAATATTTA
    ATCAAAGACA GTTGTTGGTT AATTCAGGCA TTCAGTCTTT CTTGTACAGT GAAAGGTTAT
    TTTATCCCCC AAAACACACC TGACTGTCAG TTGCCAAGCG GAGAGGCTGG AATCATTTGG
    GACAGTATAT GTTTTGCTTT CCTTTTATTG CAAAGAAGAG TGTTTATGAG CTATTACTTC
    TTACATGTGG TTGCAGACAT CAAATCATCT CAGATTCTAG CATCAAGAGG TGCTGAACTG
    TTCCAAGCTA CAATTGTAAA AGCTGTAAAA GCAAGAATTG AAGAAGAAAA AAAATCTATG
    GACCAGCTCA AGAGACAGAT GGACCACATT AAAGCCAGGC AAGAAAAATA TAAAAAAGGT
    AAAGAAAAAA TGCTGAGTCT TACACAGGAT GGAGAAGGAC AAGAAGTTCA AAAACTAGAT
    GATGACGATG AAGAAGGAGA CAAAGCAGAC AAAGAGAAAG CCAAGGGCAA AAAAAAGCAG
    TGGTGGCGAC CTTGGGTTGA TCATGCTTCC ATGGTGAAAA GCGGTGATTA TTATTTGTTT
    GAGACGGACA GTGAAGAAGA AGATGAGGAT GACAAAAAAG AAGAAGAGCC TAGAAGAAAA
    TCTGCCTTCC AGAGAGCTAT TGGAAAGTTT GCATCAGCCA TTATAGCCTT GCCAAAGTCT
    GTCATTAAAC TGCCTAAAAC AATTCTTCAA TATTTAATCA GAGCTGCAAA GTTTTTGTAC
    CAAGCATGGA TTACTGATCC AAAAACAGCC CTTAAGCAAA GAAGCATGGA AGCCAAAGAA
    GAAAAGGCAA GGGAAATGAG GCGAAAGGGC ATTGCTGAAG GTGATATTAT TGTAGAATGT
    GGGGATAAAG AAGAAGAAAA AGAGCAAGGA AAAAAGAAAT CAGATGGCCC AGACAATATC
    TTCAAAAGAA TATTTAACAT CCTGAAGTTT ACTTGGGTCC TCTTCCTTGC TACAATCGAT
    AGTTTTACGG CTTGGCTAAA CTCCATTTCA AGAGAACATA TTGATATCTC CAAAGTTCTA
    AGCATAGAAC GTTGCATGCT GACTAGAGAG ATAAAAAAGG GCAATGTGCC TACTCGAGAA
    AGCATCCACA TGTATTACCA AAATCAAATA ATGATGAGCC TTTCTAGAGA GTCTGGACTT
    GACAGAATTG ATGACCAACC TGGTTCATCG TCCAGTCACC ATAGCCATGA CAAGATGGCG
    AGTAAAAGGA TGGACAGTGC AGACTCAGCT GTATCTCATG ATAGCATATC AAGCTGCTGC
    ACTGAAGCAA CCATGCTATT CTCAAGGCAA TCTACACTTG ATGATTTAGA TGGACCAGAC
    TCTGTACCAA AAACAAGTGA GCGTGCTCGA CCTAGGCTTC GTAAAATGTA CAGCATGGAC
    ATGTCTTCTT CATCTGCTGA CAGTATAGCT TCAAGTGAGC CAACTCAGTG CACCATGGTC
    TACTCACGGC AGGGCACAAC AGAAACTATT GAGGAAGTGG AAGATGAAGA GGAGGAAGAA
    AGCAAAAGTC CTTTATCAAA GATAGAAGCC GAGGAAGAAG AATATGACAT GGATTTGGAA
    GGGACAAGCT ATACTCCGGA CACAGAATTT CCCCTGTACG CCACTACAGA GGGGGATCTT
    CCTCCTTCGT ACAGTAAAGC CGGCAGCTTC GAACAACTTA CATTTGTATC TCAGGACTCG
    GCTGACAATT TAATGGTTCT AAGCCCAGAT GAAAGTCGTA CTGATAAGCT ACATGATCAT
    ATTCTTCCAC CTCTTACACA TGAACTGACT GCTAGCGAGC TGCTTCTAAA CAAAATGTTT
    TATGATGAAG AGTTAGAAGA CTCTGAAAAA TTTTATACTA CTCAACCACG CCCGCTGCAG
    CTTATTTATG CCTTGTATAA TACATTGGTG GCCAGATCAG AAATGGTGTG CTATTTTGTG
    ATTATTCTTA ATCACATGAT TTCAGCTTCT ATGATCACAT TGGTACTGCC CATCCTTATA
    TTCCTTTGGG CTATGCTTTC TGTCCCAAGG CCCAGTAAGA GATTTTGGAT GACAGCTATT
    GTATATACAG AGGTGACAAT TGTTATTAAA TATTTTTTCC AATTTGGATT CTTCCCATGG
    AATAAAAATG TGGATTATAC AAAGGACAAA CCTTACTTCC CACCTAACAT AATGGGCATT
    GAGAAGAAAG AAGGTTATGT GCACTATGAC CTGGTGCAAC TGCTTGCTCT GTTTTTCCAT
    AGATCTATTT TAAAGTGCCA CGGGCTTTGG GATGAAGATG ATGTCACTGA AAAAGCCACA
    GATAAAGACG ACACGGATGG AGAGATATCA GAGGCATCAG GAAGAAGGGA TTCCGTTGGC
    TCATTGAAAT CTGTCAATCT AGCTGCGTCT GTGGAGTCAG TCCATGTGCA CTTCCCTGAG
    GAAAAGACCG CTATCAGGAG AAAAAGCACA AGCAGTGGAT CGCAAATTTC CCGAAGGTCA
    AGTTATTCCT CGCACAGGTC TAAAAGAGGC AGCACCAGTA CTCGGAACAG CAGTCAAAAA
    GGAAGCAGCA TTCCCAGTAT TAAACTGAAA TCTAGAAAGG AACTTATACT GGAAAAACTC
    AGAGAACAGC TCATTAAAGC AAAAGCATTT ACGATCAAGA CGATACTACA AATCTATGTC
    CCGATCAGAC AGTTTTTTTA CAATCTTATA CATCCTGAAT ACAGTGCTGT GACTGATGTC
    TATGTGCTGA TGTTCCTGGC AGACACTGTA GATTTCATCA TTATTGTTTT TGGATTTTGG
    GCGTTTGGAA AACACTCCGC TGCAGCAGAT ATTACATCCT CTCTATCAGA AGACCAAGTC
    CCAGAAGCAT TTTTGGTAAT GGTGCTTATC CAGTTTGGAA CCATGGTTGT TGATCGTGCT
    TTGTATCTTC GCAAAACAGT CATGGGAAAA GTTATTTTCC AAGTTATCCT TGTATTTGGA
    ATCCATTTTT GGATGTTCTT CATCTTGCCA GGGGTAACAG AAAGGAAATT TAGTCAGAAC
    ACTGTTGCCC AACTGTGGTA TTTTGTCAAG TGCATTTATT TTGGATTGTC GGCATATCAG
    ATACGTTGTG GATATCCAAC TCGGGTGCTT GGTAACTTCC TTACTAAAAG CTACAATTAT
    GTCAACCTGT TCCTTTTCCA AGGCTTTCGC CTCGTTCCAT TTCTTACGGA GCTGCGAGCT
    GTTATGGACT GGGTATGGAC TGACACCACT CTCAGTCTTT CCAGCTGGAT CTGTGTAGAG
    GACATTTATG CACATATATT TATACTAAAA TGCTGGCGGG AGTCAGAAAA GAGATATCCC
    CAGCCACGAG GCCAAAAGAA GAAAAAGGTT GTGAAATACG GAATGGGTGG AATGATAATA
    GTTCTGCTGA TTTGTATCGT GTGGTTTCCT TTGCTCTTCA TGTCCTTGAT TAAATCAGTT
    GCTGGGGTCA CTAACAAGCC CCTAGATGTG TCCATTACAA TAACCCTTGG AGGTTATCAG
    CCTATTTTTA CCATGAGTGC ACAACAAAAT CAGCTGCAAG GCTTGAATGA CAATGAATTT
    AAGCATTTAC ATAATATATA TAAAGGCAAT ACACCTGCCA TGCAGTTTCT GGAATCCTAT
    ATGCAGGAAG ACATCACCAT AGCAGGACTG GAAGGCAATT CCAACTCTCT GTGGACCATC
    AGCCCTCCGA GCAGGACAAT GATGATAGAA AGACTTATCC AGGAACCTGA CTTCACAGCG
    GTCATTTCTT GGTCCATTCG AAGAAATGTG AGTCTTGGAG CTAAGGCAGA GGTAACAGGT
    GAAAAATATA CAATATCACT GGCAGGTCAA ACTCGGAGGG AACTTGCGGA TATGATGAAT
    AGTTCATCTC CCAATAAGAC AGTGGAACTG ATAAAGATAT ACCCATTGTG TTTAAAAGCA
    CCAAGTGATG TTACTGCAAA ACCCATCCTT CAGCTTCTTA AGAATCAAGA GTTTGTGAAT
    ATTAGCGTTT TACTAAGAAA AGAAAACACA TCAGAAGGGG TTCGAGAATG GTGGGTAGTG
    AATCAAAGCC ATAAAGAGTT CATTAAGAAG GATGGCCTGG AACTATGGGT TTTCAGTGAC
    AAAGTCAGTC CTCCAAGTCT TGGATTCTTG GCAGGCTATG GAATAATGGG ACTATATGCC
    TCTGTAGTTC TCGTAATAGG AAAGTTTGTG CGTGAGTTTT TCAGCGGAAT TTCGCACTCC
    ATCATGTTTG AGGAGCTTCC CAACGTTGAC CGTATACTGA AGCTGTGCAC AGACATTTTC
    CTTGTGAGAG AGACTGGAGA GTTGGAACTG GAGGAAGACC TGTATGCCAA ATTAATATTT
    TTGTACCGCT CCCCAGAAAC TATGATCAAA TGGACAAGAG AGAAAGATAA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa60019
    Clone ID Related Accession (Same CDS sequence) XM_031903662.1
    Accession Version XM_031903662.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8394bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product piezo-type mechanosensitive ion channel component 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
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    1621
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    1981
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    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGGCATCGG AAGTGGTGTG TGGGCTCATC TTCAGATTTC TTCTCCCACT ATGCCTGGTT 
    GTAGCGTGCA TGTTCCGGTA CAATGGAATA TCTTTTGTCT ACCTCATCTA CCTCTTGCTT
    ATACCTTTAT TTTCGGAGCC TACCAAAATA ACAATGCAAG GTCATACAGG ACGGTTATTA
    AAATCCTTAT GTGTTTCCAG TCTGTCATTT CTGCTGCTGC ACATCATCTT TCAAATTACC
    GTATACAGCC TCGAGGCCAG AAAACACATT GAACCACATT TTAACTGTTC AACATGGGAG
    AAAGCAATAC GACAAATTGG TTTTGAAAGT GTAAAAGGAG CAGATGCTGG TAATGGAATC
    CGCGTGTTTG CACCAGATAT CGGAATGTTT ATTGCAAGCT TGGCTACCCT GTTGGTTTGT
    AGAAAACTAG TTCAGAAAAC AGTTACTGAA GAGGACGCAC AATACAACCC CCAGTTTGTA
    AATGAAGAAA TGGCAGAAGA AGGAAAGGTG GACCTGGATG AAGCGTTAAT GTATGAGGAA
    GACTTTGATG GAGAAGAGGA TGAAATTGAG GCAGCTGAAG AAAGCACAAA ATTAAAAATT
    CTTCGCAGAA TTGCTTCATT TGCATCCGTA CTTAAAGAGT TTATTGGCAA CATTATAACC
    ACAGCTGGGA AAGTAGTTAT TACAATTTTA CTTGGTTTAA CAGGCATGAT GTTGCCGTCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT TTGGGGCTAT GCACATGGTG GTCCTGTTGC
    CGAGTATTTG ATCCGCTGAT ATTCAGCTGT CTATGTGTAT TAATGGCTAT ATTTAGTGCA
    GGGCACTTAA TTGGACTTTA TCTGTACCAG TTGCAGTTTT TTCACGAGAT TATTCCACCA
    AATGATTTCT ATGCCAGGTT GTTTGGTGTT ACTTCATTAG TTCAAACCGA CTGCTGTAAC
    ACTTGGAGAT TTTTTGCAAA CAACAGTCTA CCATGGAACC AGTATACCAA TCCTGTCATG
    CTTTTAGTAC TGTATTACAC GCTGGCAACT CTCATACGCC TTTGGCTACA GGAACCTATT
    AATGTAGAGG AAGAGAAGGA GGAAGACTGT AGCCCAGCAG CACTTACTGC AGAAAGAAGA
    CGTAGTTTGT GGTACTCTAG GCACTACACA ACGGATGAAA GACAACTTCT GTCCATGACT
    CAAGATGACT ACAAGCCATC TGATGTTTTA CTAGTAACTG TAAATGGGAC TCCCGTAGAT
    TACTGTTCAC TAAATTCATC ACTTCCGATA GAAAATGGTC CAACTAAAAT TGACATGTAT
    TCAACACCGC AATATAAATG GGACCATGAA GATGAAACTT CAGAAAAGAA AGAAGAGGAA
    GAGCAAGAAG AAGAACCCAA TGAAGAGGTT GCAGAAGAGA AAGAAAACAC TAAAGTTCAT
    GTAATGGTGG TGGCATTTCA GTTCATTATG AAGCAAAGCT ATATATGTGC ACTAATTTCA
    ATGATGGCCT GGAGCATCAC ATACCACAGC TGGTTGACTT TTGTTTTGTT AATCTGGTCT
    TGTGCTCTCT GGATGGTCCG AAATAGGAGA AAATATGCTA TGCTTAGTTC ACCTTTTATG
    GTTATGTATG GTAATTTACT TTTAACATTA CAATACATAT GGAGCATAGC CTTACAAAGT
    GATGAACTTC CAGAAGTTAT GGGTCTCCTT GAGAGAAAAA AGCCCGAAGA GCTTGTATCG
    AAGATCATTT TCACAGTTAC ATTTTGGCTT TTGCTCAGAC AACACCTTAC AGAGCAAAAG
    GCATTAAAGC AAAAAGAATT AGCATTATCT GAAATAAAAG TTGGAAGTGA AGACAAAGAA
    GGTGAAGTGC AAGAAGAAGA AGAAGAAGTG GAGGATGAAA ATGATATCAT GAAGGTCCTC
    GGAAAGCTAG TGCAGGCTAT GTTTGTCAAG TACTGGATTT ATGTCTGTGG CGGCATGTTT
    TTTTTTGTCA GCTTTGAAGG AAAAATAGTT ATGTACAAAA TTATCTACAT GATGCTCTTC
    CTATTCTGTG TAGCTCTGTA TCAGGTGCAC TATGAATGGT GGAGACGAAT TTTAAAATAT
    TTTTGGATGT CTGTGGTTGT TTACACCATG TTGGTGCTTG TTTTTATTTA CACTTACCAA
    TTTAAATCAT TTCCGGGCCT TTGGATGAAT ATGACAGGGC TGGATAAGGA AAAGTTAGCA
    GATCTTGGTT TAGAGACATT TAACTTGACT GACCTATTTA CTCGTATATT TATCCCAACC
    TCATTTTTGT TAGTGTGCAT TTTGCACCTA CATTACTTCC ATGACCGTTT TCTCCAGCTC
    ACTGACCTAA AGGCTGTAAC CAGCAAGCAG GACAGCACAA TTTACAGCCA TGCCAAAGTA
    AATGGTCGAG TTTACTTAAT AATAAATAGC ATCAAGCAAA AAATTCCAAT TCAGCAAAAT
    GAATTGGTGC ATCCAGAAGG AAGTTTGCCT GATATAACGA TGATGAACTT AACAACAGAA
    TCTGAAATGC TTGAGGAAAA GCGGTTGGAA ATTTATGACG AAAAAAAAAC TCATGACCAA
    GACTCAGATG ATGAATGGGA TAAAGGGGAT AGCAAAGAAC CAGGTCATTC TGATGATGAA
    GCTGAGGATG AGGAATCAGA AGAAGAAGAA ACAACAACAG AGTCCAGGAA CAAGTGGCAT
    CTAGTGATAG ATCGCTTAAC GGTTCTTTTC CTAAAGTTTC TGGAATATTT TCACAAGTTT
    CAGGTCTTTG TTTGGTGGCT TCTGGAGCTT CACATGATAA AGATTGTTTC TTCATACATC
    ATTTGGCTTA CTATAAAGGA GGTTTCACTG CTCAATTATG TATTTTTTAT TGCCTGGGCC
    TTCGCTTTGC CCTACTCACA ATTCCGGCCA TTGGCATCAA GTGTTTGCAC AGTCTGGACA
    TGTGTCATCA TCATATGTAA AATGTTGTAC CAGCTAGCCT CCATTGACCC TGCAACCTTT
    TCCAGCAACT GTTTAATGCC ACAGCTAAAC GAAACAACTG CACGTAACCA AACTGAGCTT
    AATTCTTCTG TGCTTTACAA TTCCTCTGTT GATCCTTCTA ATTGGGTTGG ACTAAAGAAG
    TCAACTCCAC TCCTTGCTTA TTTGCGGAAC AATTTGCTGA TGTTGGCCAT GTTAGCTTTT
    GAAGTAACCA TTTACCGGCA TCAGGAGTAT TACAGGTTGC GAAACAATCT CACCACACCT
    GCAACAAAAA CCATCTTTCA TGACATCACA AGACTTCACT TGGATGATGG ACTCATAAAT
    AGTGCCAAGT ATTTTGTGAA CTACTTTTTC TACAAGTTTG GATTGGAAAC ATGTTTCTTA
    CTGTCGGTAA ATGTAATCGG TCAGAGGATG GACTTTTTTG CCATGGTTCA TGCACTTTGG
    CTTTTTGGCG TATTATACCG ACGCAGAAGG AAAGCGATTG CAGAAACCTG GCACAGATAT
    TGCTGTTTTC TTGCTTGCAT TATGGCATTC CAGTATTTTA TATGTATTGG CATCCCCCCT
    GCTGCATGTA AAGACTATCC TTGGAGACAA CCTGGAGCCA ATTTTAATTC TAACATAATA
    AAGTGGTTAT ACTTTCCAGA CTTTATTGTG AGGCCTAGTC CTCTGTTTCT TGTCTATGAT
    TTTTTGCTTT TGCTTTGTGC GTCCCTTCAA AGAGAAGTGT TTGAGGAAGA AAATAAGGCA
    GCAGTTCGAA TCATGGCTGG TGACAATGTA GAAATTTGCA TGAACCTTGA TGCTGCATCT
    TTTAGCCAAC ATAATCCTGT ACCTGATTTC ATATACTGCA GATCTTATTT GGACATGGGT
    AAAGTTATAA TGTTTAGTTA CCTTTTCTGG TTTGTGCTCA CCATCATCTT CATCACGGGA
    ACTACACGGA TCAGCATATT CTGCATGGGC TACTTGGTAG CTTGCTTCTA TTTTCTATTA
    TTTGGTGGAG ATTTGCTGTT AAAACCTATA AGAATCATCC TACGCTATTG GGATTGGCTG
    ATTGCTTACA ATGTTTTTGT CATTACAATG AAAAATGTAT TATCAATAGG AGCATGTGGC
    TACATTGAAT ATTTAATCAA AGACAGTTGT TGGTTAATTC AGGCATTCAG TCTTTCTTGT
    ACAGTGAAAG GTTATTTTAT CCCCCAAAAC ACACCTGACT GTCAGTTGCC AAGCGGAGAG
    GCTGGAATCA TTTGGGACAG TATATGTTTT GCTTTCCTTT TATTGCAAAG AAGAGTGTTT
    ATGAGCTATT ACTTCTTACA TGTGGTTGCA GACATCAAAT CATCTCAGAT TCTAGCATCA
    AGAGGTGCTG AACTGTTCCA AGCTACAATT GTAAAAGCTG TAAAAGCAAG AATTGAAGAA
    GAAAAAAAAT CTATGGACCA GCTCAAGAGA CAGATGGACC ACATTAAAGC CAGGCAAGAA
    AAATATAAAA AAGGTAAAGA AAAAATGCTG AGTCTTACAC AGGATGGAGA AGGACAAGAA
    GTTCAAAAAC TAGATGATGA CGATGAAGAA GTGGTGAAAA GCGGTGATTA TTATTTGTTT
    GAGACGGACA GTGAAGAAGA AGATGAGGAT GACAAAAAAG AAGAAGAGCC TAGAAGAAAA
    TCTGCCTTCC AGAGAGCTAT TGGAAAGTTT GCATCAGCCA TTATAGCCTT GCCAAAGTCT
    GTCATTAAAC TGCCTAAAAC AATTCTTCAA TATTTAATCA GAGCTGCAAA GTTTTTGTAC
    CAAGCATGGA TTACTGATCC AAAAACAGCC CTTAAGCAAA GAAGCATGGA AGCCAAAGAA
    GAAAAGGCAA GGGAAATGAG GCGAAAGGGC ATTGCTGAAG GTGATATTAT TGTAGAATGT
    GGGGATAAAG AAGAAGAAAA AGAGCAAGGA AAAAAGAAAT CAGATGGCCC AGACAATATC
    TTCAAAAGAA TATTTAACAT CCTGAAGTTT ACTTGGGTCC TCTTCCTTGC TACAATCGAT
    AGTTTTACGG CTTGGCTAAA CTCCATTTCA AGAGAACATA TTGATATCTC CAAAGTTCTA
    AGCATAGAAC GTTGCATGCT GACTAGAGAG ATAAAAAAGG GCAATGTGCC TACTCGAGAA
    AGCATCCACA TGTATTACCA AAATCAAATA ATGATGAGCC TTTCTAGAGA GTCTGGACTT
    GACAGAATTG ATGACCAACC TGGTTCATCG TCCAGTCACC ATAGCCATGA CAAGATGGCG
    AGTAAAAGGA TGGACAGTGC AGACTCAGCT GTATCTCATG ATAGCATATC AAGCTGCTGC
    ACTGAAGCAA CCATGCTATT CTCAAGGCAA TCTACACTTG ATGATTTAGA TGGACCAGAC
    TCTGTACCAA AAACAAGTGA GCGTGCTCGA CCTAGGCTTC GTAAAATGTA CAGCATGGAC
    ATGTCTTCTT CATCTGCTGA CAGTATAGCT TCAAGTGAGC CAACTCAGTG CACCATGGTC
    TACTCACGGC AGGGCACAAC AGAAACTATT GAGGAAGTGG AAGATGAAGA GGAGGAAGAA
    AGCAAAAGTC CTTTATCAAA GATAGAAGCC GAGGAAGAAG AATATGACAT GGATTTGGAA
    GGGACAAGCT ATACTCCGGA CACAGAATTT CCCCTGTACG CCACTACAGA GGGGGATCTT
    CCTCCTTCGT ACAGTAAAGC CGGCAGCTTC GAACAACTTA CATTTGTATC TCAGGACTCG
    GCTGACAATT TAATGGTTCT AAGCCCAGAT GAAAGTCGTA CTGATAAGCT ACATGATCAT
    ATTCTTCCAC CTCTTACACA TGAACTGACT GCTAGCGAGC TGCTTCTAAA CAAAATGTTT
    TATGATGAAG AGTTAGAAGA CTCTGAAAAA TTTTATACTA CTCAACCACG CCCGCTGCAG
    CTTATTTATG CCTTGTATAA TACATTGGTG GCCAGATCAG AAATGGTGTG CTATTTTGTG
    ATTATTCTTA ATCACATGAT TTCAGCTTCT ATGATCACAT TGGTACTGCC CATCCTTATA
    TTCCTTTGGG CTATGCTTTC TGTCCCAAGG CCCAGTAAGA GATTTTGGAT GACAGCTATT
    GTATATACAG AGGTGACAAT TGTTATTAAA TATTTTTTCC AATTTGGATT CTTCCCATGG
    AATAAAAATG TGGATTATAC AAAGGACAAA CCTTACTTCC CACCTAACAT AATGGGCATT
    GAGAAGAAAG AAGGTTATGT GCACTATGAC CTGGTGCAAC TGCTTGCTCT GTTTTTCCAT
    AGATCTATTT TAAAGTGCCA CGGGCTTTGG GATGAAGATG ATGTCACTGA AAAAGCCACA
    GATAAAGACG ACACGGATGG AGAGATATCA GAGGCATCAG GAAGAAGGGA TTCCGTTGGC
    TCATTGAAAT CTGTCAATCT AGCTGCGTCT GTGGAGTCAG TCCATGTGCA CTTCCCTGAG
    GAAAAGACCG CTATCAGGAG AAAAAGCACA AGCAGTGGAT CGCAAATTTC CCGAAGGTCA
    AGTTATTCCT CGCACAGGTC TAAAAGAGGC AGCACCAGTA CTCGGAACAG CAGTCAAAAA
    GGAAGCAGCA TTCCCAGTAT TAAACTGAAA TCTAGAAAGG AACTTATACT GGAAAAACTC
    AGAGAACAGC TCATTAAAGC AAAAGCATTT ACGATCAAGA CGATACTACA AATCTATGTC
    CCGATCAGAC AGTTTTTTTA CAATCTTATA CATCCTGAAT ACAGTGCTGT GACTGATGTC
    TATGTGCTGA TGTTCCTGGC AGACACTGTA GATTTCATCA TTATTGTTTT TGGATTTTGG
    GCGTTTGGAA AACACTCCGC TGCAGCAGAT ATTACATCCT CTCTATCAGA AGACCAAGTC
    CCAGAAGCAT TTTTGGTAAT GGTGCTTATC CAGTTTGGAA CCATGGTTGT TGATCGTGCT
    TTGTATCTTC GCAAAACAGT CATGGGAAAA GTTATTTTCC AAGTTATCCT TGTATTTGGA
    ATCCATTTTT GGATGTTCTT CATCTTGCCA GGGGTAACAG AAAGGAAATT TAGTCAGAAC
    ACTGTTGCCC AACTGTGGTA TTTTGTCAAG TGCATTTATT TTGGATTGTC GGCATATCAG
    ATACGTTGTG GATATCCAAC TCGGGTGCTT GGTAACTTCC TTACTAAAAG CTACAATTAT
    GTCAACCTGT TCCTTTTCCA AGGCTTTCGC CTCGTTCCAT TTCTTACGGA GCTGCGAGCT
    GTTATGGACT GGGTATGGAC TGACACCACT CTCAGTCTTT CCAGCTGGAT CTGTGTAGAG
    GACATTTATG CACATATATT TATACTAAAA TGCTGGCGGG AGTCAGAAAA GAGATATCCC
    CAGCCACGAG GCCAAAAGAA GAAAAAGGTT GTGAAATACG GAATGGGTGG AATGATAATA
    GTTCTGCTGA TTTGTATCGT GTGGTTTCCT TTGCTCTTCA TGTCCTTGAT TAAATCAGTT
    GCTGGGGTCA CTAACAAGCC CCTAGATGTG TCCATTACAA TAACCCTTGG AGGTTATCAG
    CCTATTTTTA CCATGAGTGC ACAACAAAAT CAGCTGCAAG GCTTGAATGA CAATGAATTT
    AAGCATTTAC ATAATATATA TAAAGGCAAT ACACCTGCCA TGCAGTTTCT GGAATCCTAT
    ATGCAGGAAG ACATCACCAT AGCAGGACTG GAAGGCAATT CCAACTCTCT GTGGACCATC
    AGCCCTCCGA GCAGGACAAT GATGATAGAA AGACTTATCC AGGAACCTGA CTTCACAGCG
    GTCATTTCTT GGTCCATTCG AAGAAATGTG AGTCTTGGAG CTAAGGCAGA GGTAACAGGT
    GAAAAATATA CAATATCACT GGCAGGTCAA ACTCGGAGGG AACTTGCGGA TATGATGAAT
    AGTTCATCTC CCAATAAGAC AGTGGAACTG ATAAAGATAT ACCCATTGTG TTTAAAAGCA
    CCAAGTGATG TTACTGCAAA ACCCATCCTT CAGCTTCTTA AGAATCAAGA GTTTGTGAAT
    ATTAGCGTTT TACTAAGAAA AGAAAACACA TCAGAAGGGG TTCGAGAATG GTGGGTAGTG
    AATCAAAGCC ATAAAGAGTT CATTAAGAAG GATGGCCTGG AACTATGGGT TTTCAGTGAC
    AAAGTCAGTC CTCCAAGTCT TGGATTCTTG GCAGGCTATG GAATAATGGG ACTATATGCC
    TCTGTAGTTC TCGTAATAGG AAAGTTTGTG CGTGAGTTTT TCAGCGGAAT TTCGCACTCC
    ATCATGTTTG AGGAGCTTCC CAACGTTGAC CGTATACTGA AGCTGTGCAC AGACATTTTC
    CTTGTGAGAG AGACTGGAGA GTTGGAACTG GAGGAAGACC TGTATGCCAA ATTAATATTT
    TTGTACCGCT CCCCAGAAAC TATGATCAAA TGGACAAGAG AGAAAGATAA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031759522.1
    CDS632..9025
    Translation

    Target ORF information:

    RefSeq Version XM_031903662.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis piezo-type mechanosensitive ion channel component 2 (piezo2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031903662.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGGCATCGG AAGTGGTGTG TGGGCTCATC TTCAGATTTC TTCTCCCACT ATGCCTGGTT 
    GTAGCGTGCA TGTTCCGGTA CAATGGAATA TCTTTTGTCT ACCTCATCTA CCTCTTGCTT
    ATACCTTTAT TTTCGGAGCC TACCAAAATA ACAATGCAAG GTCATACAGG ACGGTTATTA
    AAATCCTTAT GTGTTTCCAG TCTGTCATTT CTGCTGCTGC ACATCATCTT TCAAATTACC
    GTATACAGCC TCGAGGCCAG AAAACACATT GAACCACATT TTAACTGTTC AACATGGGAG
    AAAGCAATAC GACAAATTGG TTTTGAAAGT GTAAAAGGAG CAGATGCTGG TAATGGAATC
    CGCGTGTTTG CACCAGATAT CGGAATGTTT ATTGCAAGCT TGGCTACCCT GTTGGTTTGT
    AGAAAACTAG TTCAGAAAAC AGTTACTGAA GAGGACGCAC AATACAACCC CCAGTTTGTA
    AATGAAGAAA TGGCAGAAGA AGGAAAGGTG GACCTGGATG AAGCGTTAAT GTATGAGGAA
    GACTTTGATG GAGAAGAGGA TGAAATTGAG GCAGCTGAAG AAAGCACAAA ATTAAAAATT
    CTTCGCAGAA TTGCTTCATT TGCATCCGTA CTTAAAGAGT TTATTGGCAA CATTATAACC
    ACAGCTGGGA AAGTAGTTAT TACAATTTTA CTTGGTTTAA CAGGCATGAT GTTGCCGTCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT TTGGGGCTAT GCACATGGTG GTCCTGTTGC
    CGAGTATTTG ATCCGCTGAT ATTCAGCTGT CTATGTGTAT TAATGGCTAT ATTTAGTGCA
    GGGCACTTAA TTGGACTTTA TCTGTACCAG TTGCAGTTTT TTCACGAGAT TATTCCACCA
    AATGATTTCT ATGCCAGGTT GTTTGGTGTT ACTTCATTAG TTCAAACCGA CTGCTGTAAC
    ACTTGGAGAT TTTTTGCAAA CAACAGTCTA CCATGGAACC AGTATACCAA TCCTGTCATG
    CTTTTAGTAC TGTATTACAC GCTGGCAACT CTCATACGCC TTTGGCTACA GGAACCTATT
    AATGTAGAGG AAGAGAAGGA GGAAGACTGT AGCCCAGCAG CACTTACTGC AGAAAGAAGA
    CGTAGTTTGT GGTACTCTAG GCACTACACA ACGGATGAAA GACAACTTCT GTCCATGACT
    CAAGATGACT ACAAGCCATC TGATGTTTTA CTAGTAACTG TAAATGGGAC TCCCGTAGAT
    TACTGTTCAC TAAATTCATC ACTTCCGATA GAAAATGGTC CAACTAAAAT TGACATGTAT
    TCAACACCGC AATATAAATG GGACCATGAA GATGAAACTT CAGAAAAGAA AGAAGAGGAA
    GAGCAAGAAG AAGAACCCAA TGAAGAGGTT GCAGAAGAGA AAGAAAACAC TAAAGTTCAT
    GTAATGGTGG TGGCATTTCA GTTCATTATG AAGCAAAGCT ATATATGTGC ACTAATTTCA
    ATGATGGCCT GGAGCATCAC ATACCACAGC TGGTTGACTT TTGTTTTGTT AATCTGGTCT
    TGTGCTCTCT GGATGGTCCG AAATAGGAGA AAATATGCTA TGCTTAGTTC ACCTTTTATG
    GTTATGTATG GTAATTTACT TTTAACATTA CAATACATAT GGAGCATAGC CTTACAAAGT
    GATGAACTTC CAGAAGTTAT GGGTCTCCTT GAGAGAAAAA AGCCCGAAGA GCTTGTATCG
    AAGATCATTT TCACAGTTAC ATTTTGGCTT TTGCTCAGAC AACACCTTAC AGAGCAAAAG
    GCATTAAAGC AAAAAGAATT AGCATTATCT GAAATAAAAG TTGGAAGTGA AGACAAAGAA
    GGTGAAGTGC AAGAAGAAGA AGAAGAAGTG GAGGATGAAA ATGATATCAT GAAGGTCCTC
    GGAAAGCTAG TGCAGGCTAT GTTTGTCAAG TACTGGATTT ATGTCTGTGG CGGCATGTTT
    TTTTTTGTCA GCTTTGAAGG AAAAATAGTT ATGTACAAAA TTATCTACAT GATGCTCTTC
    CTATTCTGTG TAGCTCTGTA TCAGGTGCAC TATGAATGGT GGAGACGAAT TTTAAAATAT
    TTTTGGATGT CTGTGGTTGT TTACACCATG TTGGTGCTTG TTTTTATTTA CACTTACCAA
    TTTAAATCAT TTCCGGGCCT TTGGATGAAT ATGACAGGGC TGGATAAGGA AAAGTTAGCA
    GATCTTGGTT TAGAGACATT TAACTTGACT GACCTATTTA CTCGTATATT TATCCCAACC
    TCATTTTTGT TAGTGTGCAT TTTGCACCTA CATTACTTCC ATGACCGTTT TCTCCAGCTC
    ACTGACCTAA AGGCTGTAAC CAGCAAGCAG GACAGCACAA TTTACAGCCA TGCCAAAGTA
    AATGGTCGAG TTTACTTAAT AATAAATAGC ATCAAGCAAA AAATTCCAAT TCAGCAAAAT
    GAATTGGTGC ATCCAGAAGG AAGTTTGCCT GATATAACGA TGATGAACTT AACAACAGAA
    TCTGAAATGC TTGAGGAAAA GCGGTTGGAA ATTTATGACG AAAAAAAAAC TCATGACCAA
    GACTCAGATG ATGAATGGGA TAAAGGGGAT AGCAAAGAAC CAGGTCATTC TGATGATGAA
    GCTGAGGATG AGGAATCAGA AGAAGAAGAA ACAACAACAG AGTCCAGGAA CAAGTGGCAT
    CTAGTGATAG ATCGCTTAAC GGTTCTTTTC CTAAAGTTTC TGGAATATTT TCACAAGTTT
    CAGGTCTTTG TTTGGTGGCT TCTGGAGCTT CACATGATAA AGATTGTTTC TTCATACATC
    ATTTGGCTTA CTATAAAGGA GGTTTCACTG CTCAATTATG TATTTTTTAT TGCCTGGGCC
    TTCGCTTTGC CCTACTCACA ATTCCGGCCA TTGGCATCAA GTGTTTGCAC AGTCTGGACA
    TGTGTCATCA TCATATGTAA AATGTTGTAC CAGCTAGCCT CCATTGACCC TGCAACCTTT
    TCCAGCAACT GTTTAATGCC ACAGCTAAAC GAAACAACTG CACGTAACCA AACTGAGCTT
    AATTCTTCTG TGCTTTACAA TTCCTCTGTT GATCCTTCTA ATTGGGTTGG ACTAAAGAAG
    TCAACTCCAC TCCTTGCTTA TTTGCGGAAC AATTTGCTGA TGTTGGCCAT GTTAGCTTTT
    GAAGTAACCA TTTACCGGCA TCAGGAGTAT TACAGGTTGC GAAACAATCT CACCACACCT
    GCAACAAAAA CCATCTTTCA TGACATCACA AGACTTCACT TGGATGATGG ACTCATAAAT
    AGTGCCAAGT ATTTTGTGAA CTACTTTTTC TACAAGTTTG GATTGGAAAC ATGTTTCTTA
    CTGTCGGTAA ATGTAATCGG TCAGAGGATG GACTTTTTTG CCATGGTTCA TGCACTTTGG
    CTTTTTGGCG TATTATACCG ACGCAGAAGG AAAGCGATTG CAGAAACCTG GCACAGATAT
    TGCTGTTTTC TTGCTTGCAT TATGGCATTC CAGTATTTTA TATGTATTGG CATCCCCCCT
    GCTGCATGTA AAGACTATCC TTGGAGACAA CCTGGAGCCA ATTTTAATTC TAACATAATA
    AAGTGGTTAT ACTTTCCAGA CTTTATTGTG AGGCCTAGTC CTCTGTTTCT TGTCTATGAT
    TTTTTGCTTT TGCTTTGTGC GTCCCTTCAA AGAGAAGTGT TTGAGGAAGA AAATAAGGCA
    GCAGTTCGAA TCATGGCTGG TGACAATGTA GAAATTTGCA TGAACCTTGA TGCTGCATCT
    TTTAGCCAAC ATAATCCTGT ACCTGATTTC ATATACTGCA GATCTTATTT GGACATGGGT
    AAAGTTATAA TGTTTAGTTA CCTTTTCTGG TTTGTGCTCA CCATCATCTT CATCACGGGA
    ACTACACGGA TCAGCATATT CTGCATGGGC TACTTGGTAG CTTGCTTCTA TTTTCTATTA
    TTTGGTGGAG ATTTGCTGTT AAAACCTATA AGAATCATCC TACGCTATTG GGATTGGCTG
    ATTGCTTACA ATGTTTTTGT CATTACAATG AAAAATGTAT TATCAATAGG AGCATGTGGC
    TACATTGAAT ATTTAATCAA AGACAGTTGT TGGTTAATTC AGGCATTCAG TCTTTCTTGT
    ACAGTGAAAG GTTATTTTAT CCCCCAAAAC ACACCTGACT GTCAGTTGCC AAGCGGAGAG
    GCTGGAATCA TTTGGGACAG TATATGTTTT GCTTTCCTTT TATTGCAAAG AAGAGTGTTT
    ATGAGCTATT ACTTCTTACA TGTGGTTGCA GACATCAAAT CATCTCAGAT TCTAGCATCA
    AGAGGTGCTG AACTGTTCCA AGCTACAATT GTAAAAGCTG TAAAAGCAAG AATTGAAGAA
    GAAAAAAAAT CTATGGACCA GCTCAAGAGA CAGATGGACC ACATTAAAGC CAGGCAAGAA
    AAATATAAAA AAGGTAAAGA AAAAATGCTG AGTCTTACAC AGGATGGAGA AGGACAAGAA
    GTTCAAAAAC TAGATGATGA CGATGAAGAA GTGGTGAAAA GCGGTGATTA TTATTTGTTT
    GAGACGGACA GTGAAGAAGA AGATGAGGAT GACAAAAAAG AAGAAGAGCC TAGAAGAAAA
    TCTGCCTTCC AGAGAGCTAT TGGAAAGTTT GCATCAGCCA TTATAGCCTT GCCAAAGTCT
    GTCATTAAAC TGCCTAAAAC AATTCTTCAA TATTTAATCA GAGCTGCAAA GTTTTTGTAC
    CAAGCATGGA TTACTGATCC AAAAACAGCC CTTAAGCAAA GAAGCATGGA AGCCAAAGAA
    GAAAAGGCAA GGGAAATGAG GCGAAAGGGC ATTGCTGAAG GTGATATTAT TGTAGAATGT
    GGGGATAAAG AAGAAGAAAA AGAGCAAGGA AAAAAGAAAT CAGATGGCCC AGACAATATC
    TTCAAAAGAA TATTTAACAT CCTGAAGTTT ACTTGGGTCC TCTTCCTTGC TACAATCGAT
    AGTTTTACGG CTTGGCTAAA CTCCATTTCA AGAGAACATA TTGATATCTC CAAAGTTCTA
    AGCATAGAAC GTTGCATGCT GACTAGAGAG ATAAAAAAGG GCAATGTGCC TACTCGAGAA
    AGCATCCACA TGTATTACCA AAATCAAATA ATGATGAGCC TTTCTAGAGA GTCTGGACTT
    GACAGAATTG ATGACCAACC TGGTTCATCG TCCAGTCACC ATAGCCATGA CAAGATGGCG
    AGTAAAAGGA TGGACAGTGC AGACTCAGCT GTATCTCATG ATAGCATATC AAGCTGCTGC
    ACTGAAGCAA CCATGCTATT CTCAAGGCAA TCTACACTTG ATGATTTAGA TGGACCAGAC
    TCTGTACCAA AAACAAGTGA GCGTGCTCGA CCTAGGCTTC GTAAAATGTA CAGCATGGAC
    ATGTCTTCTT CATCTGCTGA CAGTATAGCT TCAAGTGAGC CAACTCAGTG CACCATGGTC
    TACTCACGGC AGGGCACAAC AGAAACTATT GAGGAAGTGG AAGATGAAGA GGAGGAAGAA
    AGCAAAAGTC CTTTATCAAA GATAGAAGCC GAGGAAGAAG AATATGACAT GGATTTGGAA
    GGGACAAGCT ATACTCCGGA CACAGAATTT CCCCTGTACG CCACTACAGA GGGGGATCTT
    CCTCCTTCGT ACAGTAAAGC CGGCAGCTTC GAACAACTTA CATTTGTATC TCAGGACTCG
    GCTGACAATT TAATGGTTCT AAGCCCAGAT GAAAGTCGTA CTGATAAGCT ACATGATCAT
    ATTCTTCCAC CTCTTACACA TGAACTGACT GCTAGCGAGC TGCTTCTAAA CAAAATGTTT
    TATGATGAAG AGTTAGAAGA CTCTGAAAAA TTTTATACTA CTCAACCACG CCCGCTGCAG
    CTTATTTATG CCTTGTATAA TACATTGGTG GCCAGATCAG AAATGGTGTG CTATTTTGTG
    ATTATTCTTA ATCACATGAT TTCAGCTTCT ATGATCACAT TGGTACTGCC CATCCTTATA
    TTCCTTTGGG CTATGCTTTC TGTCCCAAGG CCCAGTAAGA GATTTTGGAT GACAGCTATT
    GTATATACAG AGGTGACAAT TGTTATTAAA TATTTTTTCC AATTTGGATT CTTCCCATGG
    AATAAAAATG TGGATTATAC AAAGGACAAA CCTTACTTCC CACCTAACAT AATGGGCATT
    GAGAAGAAAG AAGGTTATGT GCACTATGAC CTGGTGCAAC TGCTTGCTCT GTTTTTCCAT
    AGATCTATTT TAAAGTGCCA CGGGCTTTGG GATGAAGATG ATGTCACTGA AAAAGCCACA
    GATAAAGACG ACACGGATGG AGAGATATCA GAGGCATCAG GAAGAAGGGA TTCCGTTGGC
    TCATTGAAAT CTGTCAATCT AGCTGCGTCT GTGGAGTCAG TCCATGTGCA CTTCCCTGAG
    GAAAAGACCG CTATCAGGAG AAAAAGCACA AGCAGTGGAT CGCAAATTTC CCGAAGGTCA
    AGTTATTCCT CGCACAGGTC TAAAAGAGGC AGCACCAGTA CTCGGAACAG CAGTCAAAAA
    GGAAGCAGCA TTCCCAGTAT TAAACTGAAA TCTAGAAAGG AACTTATACT GGAAAAACTC
    AGAGAACAGC TCATTAAAGC AAAAGCATTT ACGATCAAGA CGATACTACA AATCTATGTC
    CCGATCAGAC AGTTTTTTTA CAATCTTATA CATCCTGAAT ACAGTGCTGT GACTGATGTC
    TATGTGCTGA TGTTCCTGGC AGACACTGTA GATTTCATCA TTATTGTTTT TGGATTTTGG
    GCGTTTGGAA AACACTCCGC TGCAGCAGAT ATTACATCCT CTCTATCAGA AGACCAAGTC
    CCAGAAGCAT TTTTGGTAAT GGTGCTTATC CAGTTTGGAA CCATGGTTGT TGATCGTGCT
    TTGTATCTTC GCAAAACAGT CATGGGAAAA GTTATTTTCC AAGTTATCCT TGTATTTGGA
    ATCCATTTTT GGATGTTCTT CATCTTGCCA GGGGTAACAG AAAGGAAATT TAGTCAGAAC
    ACTGTTGCCC AACTGTGGTA TTTTGTCAAG TGCATTTATT TTGGATTGTC GGCATATCAG
    ATACGTTGTG GATATCCAAC TCGGGTGCTT GGTAACTTCC TTACTAAAAG CTACAATTAT
    GTCAACCTGT TCCTTTTCCA AGGCTTTCGC CTCGTTCCAT TTCTTACGGA GCTGCGAGCT
    GTTATGGACT GGGTATGGAC TGACACCACT CTCAGTCTTT CCAGCTGGAT CTGTGTAGAG
    GACATTTATG CACATATATT TATACTAAAA TGCTGGCGGG AGTCAGAAAA GAGATATCCC
    CAGCCACGAG GCCAAAAGAA GAAAAAGGTT GTGAAATACG GAATGGGTGG AATGATAATA
    GTTCTGCTGA TTTGTATCGT GTGGTTTCCT TTGCTCTTCA TGTCCTTGAT TAAATCAGTT
    GCTGGGGTCA CTAACAAGCC CCTAGATGTG TCCATTACAA TAACCCTTGG AGGTTATCAG
    CCTATTTTTA CCATGAGTGC ACAACAAAAT CAGCTGCAAG GCTTGAATGA CAATGAATTT
    AAGCATTTAC ATAATATATA TAAAGGCAAT ACACCTGCCA TGCAGTTTCT GGAATCCTAT
    ATGCAGGAAG ACATCACCAT AGCAGGACTG GAAGGCAATT CCAACTCTCT GTGGACCATC
    AGCCCTCCGA GCAGGACAAT GATGATAGAA AGACTTATCC AGGAACCTGA CTTCACAGCG
    GTCATTTCTT GGTCCATTCG AAGAAATGTG AGTCTTGGAG CTAAGGCAGA GGTAACAGGT
    GAAAAATATA CAATATCACT GGCAGGTCAA ACTCGGAGGG AACTTGCGGA TATGATGAAT
    AGTTCATCTC CCAATAAGAC AGTGGAACTG ATAAAGATAT ACCCATTGTG TTTAAAAGCA
    CCAAGTGATG TTACTGCAAA ACCCATCCTT CAGCTTCTTA AGAATCAAGA GTTTGTGAAT
    ATTAGCGTTT TACTAAGAAA AGAAAACACA TCAGAAGGGG TTCGAGAATG GTGGGTAGTG
    AATCAAAGCC ATAAAGAGTT CATTAAGAAG GATGGCCTGG AACTATGGGT TTTCAGTGAC
    AAAGTCAGTC CTCCAAGTCT TGGATTCTTG GCAGGCTATG GAATAATGGG ACTATATGCC
    TCTGTAGTTC TCGTAATAGG AAAGTTTGTG CGTGAGTTTT TCAGCGGAAT TTCGCACTCC
    ATCATGTTTG AGGAGCTTCC CAACGTTGAC CGTATACTGA AGCTGTGCAC AGACATTTTC
    CTTGTGAGAG AGACTGGAGA GTTGGAACTG GAGGAAGACC TGTATGCCAA ATTAATATTT
    TTGTACCGCT CCCCAGAAAC TATGATCAAA TGGACAAGAG AGAAAGATAA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa60020
    Clone ID Related Accession (Same CDS sequence) XM_012965361.3
    Accession Version XM_012965361.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8370bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product piezo-type mechanosensitive ion channel component 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012965361.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGGCATCGG AAGTGGTGTG TGGGCTCATC TTCAGATTTC TTCTCCCACT ATGCCTGGTT 
    GTAGCGTGCA TGTTCCGGTA CAATGGAATA TCTTTTGTCT ACCTCATCTA CCTCTTGCTT
    ATACCTTTAT TTTCGGAGCC TACCAAAATA ACAATGCAAG GTCATACAGG ACGGTTATTA
    AAATCCTTAT GTGTTTCCAG TCTGTCATTT CTGCTGCTGC ACATCATCTT TCAAATTACC
    GTATACAGCC TCGAGGCCAG AAAACACATT GAACCACATT TTAACTGTTC AACATGGGAG
    AAAGCAATAC GACAAATTGG TTTTGAAAGT GTAAAAGGAG CAGATGCTGG TAATGGAATC
    CGCGTGTTTG CACCAGATAT CGGAATGTTT ATTGCAAGCT TGGCTACCCT GTTGGTTTGT
    AGAAAACTAG TTCAGAAAAC AGTTACTGAA GAGGACGCAC AATACAACCC CCAGTTTGTA
    AATGAAGAAA TGGCAGAAGA AGGAAAGGTG GACCTGGATG AAGCGTTAAT GTATGAGGAA
    GACTTTGATG GAGAAGAGGA TGAAATTGAG GCAGCTGAAG AAAGCACAAA ATTAAAAATT
    CTTCGCAGAA TTGCTTCATT TGCATCCGTA CTTAAAGAGT TTATTGGCAA CATTATAACC
    ACAGCTGGGA AAGTAGTTAT TACAATTTTA CTTGGTTTAA CAGGCATGAT GTTGCCGTCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT TTGGGGCTAT GCACATGGTG GTCCTGTTGC
    CGAGTATTTG ATCCGCTGAT ATTCAGCTGT CTATGTGTAT TAATGGCTAT ATTTAGTGCA
    GGGCACTTAA TTGGACTTTA TCTGTACCAG TTGCAGTTTT TTCACGAGAT TATTCCACCA
    AATGATTTCT ATGCCAGGTT GTTTGGTGTT ACTTCATTAG TTCAAACCGA CTGCTGTAAC
    ACTTGGAGAT TTTTTGCAAA CAACAGTCTA CCATGGAACC AGTATACCAA TCCTGTCATG
    CTTTTAGTAC TGTATTACAC GCTGGCAACT CTCATACGCC TTTGGCTACA GGAACCTATT
    AATGTAGAGG AAGAGAAGGA GGAAGACTGT AGCCCAGCAG CACTTACTGC AGAAAGAAGA
    CGTAGTTTGT GGTACTCTAG GCACTACACA ACGGATGAAA GACAACTTCT GTCCATGACT
    CAAGATGACT ACAAGCCATC TGATGTTTTA CTAGTAACTG TAAATGGGAC TCCCGTAGAT
    TACTGTTCAC TAAATTCATC ACTTCCGATA GAAAATGGTC CAACTAAAAT TGACATGTAT
    TCAACACCGC AATATAAATG GGACCATGAA GATGAAACTT CAGAAAAGAA AGAAGAGGAA
    GAGCAAGAAG AAGAACCCAA TGAAGAGGTT GCAGAAGAGA AAGAAAACAC TAAAGTTCAT
    GTAATGGTGG TGGCATTTCA GTTCATTATG AAGCAAAGCT ATATATGTGC ACTAATTTCA
    ATGATGGCCT GGAGCATCAC ATACCACAGC TGGTTGACTT TTGTTTTGTT AATCTGGTCT
    TGTGCTCTCT GGATGGTCCG AAATAGGAGA AAATATGCTA TGCTTAGTTC ACCTTTTATG
    GTTATGTATG GTAATTTACT TTTAACATTA CAATACATAT GGAGCATAGC CTTACAAAGT
    GATGAACTTC CAGAAGTTAT GGGTCTCCTT GAGAGAAAAA AGCCCGAAGA GCTTGTATCG
    AAGATCATTT TCACAGTTAC ATTTTGGCTT TTGCTCAGAC AACACCTTAC AGAGCAAAAG
    GCATTAAAGC AAAAAGAATT AGCATTATCT GAAATAAAAG TTGGAAGTGA AGACAAAGAA
    GGTGAAGTGC AAGAAGAAGA AGAAGAAGTG GAGGATGAAA ATGATATCAT GAAGGTCCTC
    GGAAAGCTAG TGCAGGCTAT GTTTGTCAAG TACTGGATTT ATGTCTGTGG CGGCATGTTT
    TTTTTTGTCA GCTTTGAAGG AAAAATAGTT ATGTACAAAA TTATCTACAT GATGCTCTTC
    CTATTCTGTG TAGCTCTGTA TCAGGTGCAC TATGAATGGT GGAGACGAAT TTTAAAATAT
    TTTTGGATGT CTGTGGTTGT TTACACCATG TTGGTGCTTG TTTTTATTTA CACTTACCAA
    TTTAAATCAT TTCCGGGCCT TTGGATGAAT ATGACAGGGC TGGATAAGGA AAAGTTAGCA
    GATCTTGGTT TAGAGACATT TAACTTGACT GACCTATTTA CTCGTATATT TATCCCAACC
    TCATTTTTGT TAGTGTGCAT TTTGCACCTA CATTACTTCC ATGACCGTTT TCTCCAGCTC
    ACTGACCTAA AGGCTGTAAC CAGCAAGCAG GACAGCACAA TTTACAGCCA TGCCAAAGTA
    AATGGTCGAG TTTACTTAAT AATAAATAGC ATCAAGCAAA AAATTCCAAT TCAGCAAAAT
    GAATTGGTGC ATCCAGAAGG AAGTTTGCCT GATATAACGA TGATGAACTT AACAACAGAA
    TCTGAAATGC TTGAGGAAAA GCGGTTGGAA ATTTATGACG AAAAAAAAAC TCATGACCAA
    GACTCAGATG ATGAATGGGA TAAAGGGGAT AGCAAAGAAC CAGGTCATTC TGATGATGAA
    GCTGAGGATG AGGAATCAGA AGAAGAAGAA ACAACAACAG AGTCCAGGAA CAAGTGGCAT
    CTAGTGATAG ATCGCTTAAC GGTTCTTTTC CTAAAGTTTC TGGAATATTT TCACAAGTTT
    CAGGTCTTTG TTTGGTGGCT TCTGGAGCTT CACATGATAA AGATTGTTTC TTCATACATC
    ATTTGGCTTA CTATAAAGGA GGTTTCACTG CTCAATTATG TATTTTTTAT TGCCTGGGCC
    TTCGCTTTGC CCTACTCACA ATTCCGGCCA TTGGCATCAA GTGTTTGCAC AGTCTGGACA
    TGTGTCATCA TCATATGTAA AATGTTGTAC CAGCTAGCCT CCATTGACCC TGCAACCTTT
    TCCAGCAACT GTTTAATGCC ACAGCTAAAC GAAACAACTG CACGTAACCA AACTGAGCTT
    AATTCTTCTG TGCTTTACAA TTCCTCTGTT GATCCTTCTA ATTGGGTTGG ACTAAAGAAG
    TCAACTCCAC TCCTTGCTTA TTTGCGGAAC AATTTGCTGA TGTTGGCCAT GTTAGCTTTT
    GAAGTAACCA TTTACCGGCA TCAGGAGTAT TACAGGTTGC GAAACAATCT CACCACACCT
    GCAACAAAAA CCATCTTTCA TGACATCACA AGACTTCACT TGGATGATGG ACTCATAAAT
    AGTGCCAAGT ATTTTGTGAA CTACTTTTTC TACAAGTTTG GATTGGAAAC ATGTTTCTTA
    CTGTCGGTAA ATGTAATCGG TCAGAGGATG GACTTTTTTG CCATGGTTCA TGCACTTTGG
    CTTTTTGGCG TATTATACCG ACGCAGAAGG AAAGCGATTG CAGAAACCTG GCACAGATAT
    TGCTGTTTTC TTGCTTGCAT TATGGCATTC CAGTATTTTA TATGTATTGG CATCCCCCCT
    GCTGCATGTA AAGACTATCC TTGGAGACAA CCTGGAGCCA ATTTTAATTC TAACATAATA
    AAGTGGTTAT ACTTTCCAGA CTTTATTGTG AGGCCTAGTC CTCTGTTTCT TGTCTATGAT
    TTTTTGCTTT TGCTTTGTGC GTCCCTTCAA AGAGAAGTGT TTGAGGAAGA AAATAAGGCA
    GCAGTTCGAA TCATGGCTGG TGACAATGTA GAAATTTGCA TGAACCTTGA TGCTGCATCT
    TTTAGCCAAC ATAATCCTGT ACCTGATTTC ATATACTGCA GATCTTATTT GGACATGGGT
    AAAGTTATAA TGTTTAGTTA CCTTTTCTGG TTTGTGCTCA CCATCATCTT CATCACGGGA
    ACTACACGGA TCAGCATATT CTGCATGGGC TACTTGGTAG CTTGCTTCTA TTTTCTATTA
    TTTGGTGGAG ATTTGCTGTT AAAACCTATA AGAATCATCC TACGCTATTG GGATTGGCTG
    ATTGCTTACA ATGTTTTTGT CATTACAATG AAAAATGTAT TATCAATAGG AGCATGTGGC
    TACATTGAAT ATTTAATCAA AGACAGTTGT TGGTTAATTC AGGCATTCAG TCTTTCTTGT
    ACAGTGAAAG GTTATTTTAT CCCCCAAAAC ACACCTGACT GTCAGTTGCC AAGCGGAGAG
    GCTGGAATCA TTTGGGACAG TATATGTTTT GCTTTCCTTT TATTGCAAAG AAGAGTGTTT
    ATGAGCTATT ACTTCTTACA TGTGGTTGCA GACATCAAAT CATCTCAGAT TCTAGCATCA
    AGAGGTGCTG AACTGTTCCA AGCTACAATT GTAAAAGCTG TAAAAGCAAG AATTGAAGAA
    GAAAAAAAAT CTATGGACCA GCTCAAGAGA CAGATGGACC ACATTAAAGC CAGGCAAGAA
    AAATATAAAA AAGGTAAAGA AAAAATGCTG AGTCTTACAC AGGATGGAGA AGGACAAGAA
    GTTCAAAAAC TAGATGATGA CGATGAAGAA GGAGACAAAG CAGACAAAGA GAAAGCCAAG
    GGCAAAAAAA AGCAGTGGTG GCGACCTTGG GTTGATCATG CTTCCATGGT GAAAAGCGGT
    GATTATTATT TGTTTGAGAC GGACAGTGAA GAAGAAGATG AGGATGACAA AAAAGAAGAA
    GAGCCTAGAA GAAAATCTGC CTTCCAGTTT TTGTACCAAG CATGGATTAC TGATCCAAAA
    ACAGCCCTTA AGCAAAGAAG CATGGAAGCC AAAGAAGAAA AGGCAAGGGA AATGAGGCGA
    AAGGGCATTG CTGAAGGTGA TATTATTGTA GAATGTGGGG ATAAAGAAGA AGAAAAAGAG
    CAAGGAAAAA AGAAATCAGA TGGCCCAGAC AATATCTTCA AAAGAATATT TAACATCCTG
    AAGTTTACTT GGGTCCTCTT CCTTGCTACA ATCGATAGTT TTACGGCTTG GCTAAACTCC
    ATTTCAAGAG AACATATTGA TATCTCCAAA GTTCTAAGCA TAGAACGTTG CATGCTGACT
    AGAGAGATAA AAAAGGGCAA TGTGCCTACT CGAGAAAGCA TCCACATGTA TTACCAAAAT
    CAAATAATGA TGAGCCTTTC TAGAGAGTCT GGACTTGACA GAATTGATGA CCAACCTGGT
    TCATCGTCCA GTCACCATAG CCATGACAAG ATGGCGAGTA AAAGGATGGA CAGTGCAGAC
    TCAGCTGTAT CTCATGATAG CATATCAAGC TGCTGCACTG AAGCAACCAT GCTATTCTCA
    AGGCAATCTA CACTTGATGA TTTAGATGGA CCAGACTCTG TACCAAAAAC AAGTGAGCGT
    GCTCGACCTA GGCTTCGTAA AATGTACAGC ATGGACATGT CTTCTTCATC TGCTGACAGT
    ATAGCTTCAA GTGAGCCAAC TCAGTGCACC ATGGTCTACT CACGGCAGGG CACAACAGAA
    ACTATTGAGG AAGTGGAAGA TGAAGAGGAG GAAGAAAGCA AAAGTCCTTT ATCAAAGATA
    GAAGCCGAGG AAGAAGAATA TGACATGGAT TTGGAAGGGA CAAGCTATAC TCCGGACACA
    GAATTTCCCC TGTACGCCAC TACAGAGGGG GATCTTCCTC CTTCGTACAG TAAAGCCGGC
    AGCTTCGAAC AACTTACATT TGTATCTCAG GACTCGGCTG ACAATTTAAT GGTTCTAAGC
    CCAGATGAAA GTCGTACTGA TAAGCTACAT GATCATATTC TTCCACCTCT TACACATGAA
    CTGACTGCTA GCGAGCTGCT TCTAAACAAA ATGTTTTATG ATGAAGAGTT AGAAGACTCT
    GAAAAATTTT ATACTACTCA ACCACGCCCG CTGCAGCTTA TTTATGCCTT GTATAATACA
    TTGGTGGCCA GATCAGAAAT GGTGTGCTAT TTTGTGATTA TTCTTAATCA CATGATTTCA
    GCTTCTATGA TCACATTGGT ACTGCCCATC CTTATATTCC TTTGGGCTAT GCTTTCTGTC
    CCAAGGCCCA GTAAGAGATT TTGGATGACA GCTATTGTAT ATACAGAGGT GACAATTGTT
    ATTAAATATT TTTTCCAATT TGGATTCTTC CCATGGAATA AAAATGTGGA TTATACAAAG
    GACAAACCTT ACTTCCCACC TAACATAATG GGCATTGAGA AGAAAGAAGG TTATGTGCAC
    TATGACCTGG TGCAACTGCT TGCTCTGTTT TTCCATAGAT CTATTTTAAA GTGCCACGGG
    CTTTGGGATG AAGATGATGT CACTGAAAAA GCCACAGATA AAGACGACAC GGATGGAGAG
    ATATCAGAGG CATCAGGAAG AAGGGATTCC GTTGGCTCAT TGAAATCTGT CAATCTAGCT
    GCGTCTGTGG AGTCAGTCCA TGTGCACTTC CCTGAGGAAA AGACCGCTAT CAGGAGAAAA
    AGCACAAGCA GTGGATCGCA AATTTCCCGA AGGTCAAGTT ATTCCTCGCA CAGGTCTAAA
    AGAGGCAGCA CCAGTACTCG GAACAGCAGT CAAAAAGGAA GCAGCATTCC CAGTATTAAA
    CTGAAATCTA GAAAGGAACT TATACTGGAA AAACTCAGAG AACAGCTCAT TAAAGCAAAA
    GCATTTACGA TCAAGACGAT ACTACAAATC TATGTCCCGA TCAGACAGTT TTTTTACAAT
    CTTATACATC CTGAATACAG TGCTGTGACT GATGTCTATG TGCTGATGTT CCTGGCAGAC
    ACTGTAGATT TCATCATTAT TGTTTTTGGA TTTTGGGCGT TTGGAAAACA CTCCGCTGCA
    GCAGATATTA CATCCTCTCT ATCAGAAGAC CAAGTCCCAG AAGCATTTTT GGTAATGGTG
    CTTATCCAGT TTGGAACCAT GGTTGTTGAT CGTGCTTTGT ATCTTCGCAA AACAGTCATG
    GGAAAAGTTA TTTTCCAAGT TATCCTTGTA TTTGGAATCC ATTTTTGGAT GTTCTTCATC
    TTGCCAGGGG TAACAGAAAG GAAATTTAGT CAGAACACTG TTGCCCAACT GTGGTATTTT
    GTCAAGTGCA TTTATTTTGG ATTGTCGGCA TATCAGATAC GTTGTGGATA TCCAACTCGG
    GTGCTTGGTA ACTTCCTTAC TAAAAGCTAC AATTATGTCA ACCTGTTCCT TTTCCAAGGC
    TTTCGCCTCG TTCCATTTCT TACGGAGCTG CGAGCTGTTA TGGACTGGGT ATGGACTGAC
    ACCACTCTCA GTCTTTCCAG CTGGATCTGT GTAGAGGACA TTTATGCACA TATATTTATA
    CTAAAATGCT GGCGGGAGTC AGAAAAGAGA TATCCCCAGC CACGAGGCCA AAAGAAGAAA
    AAGGTTGTGA AATACGGAAT GGGTGGAATG ATAATAGTTC TGCTGATTTG TATCGTGTGG
    TTTCCTTTGC TCTTCATGTC CTTGATTAAA TCAGTTGCTG GGGTCACTAA CAAGCCCCTA
    GATGTGTCCA TTACAATAAC CCTTGGAGGT TATCAGCCTA TTTTTACCAT GAGTGCACAA
    CAAAATCAGC TGCAAGGCTT GAATGACAAT GAATTTAAGC ATTTACATAA TATATATAAA
    GGCAATACAC CTGCCATGCA GTTTCTGGAA TCCTATATGC AGGAAGACAT CACCATAGCA
    GGACTGGAAG GCAATTCCAA CTCTCTGTGG ACCATCAGCC CTCCGAGCAG GACAATGATG
    ATAGAAAGAC TTATCCAGGA ACCTGACTTC ACAGCGGTCA TTTCTTGGTC CATTCGAAGA
    AATGTGAGTC TTGGAGCTAA GGCAGAGGTA ACAGGTGAAA AATATACAAT ATCACTGGCA
    GGTCAAACTC GGAGGGAACT TGCGGATATG ATGAATAGTT CATCTCCCAA TAAGACAGTG
    GAACTGATAA AGATATACCC ATTGTGTTTA AAAGCACCAA GTGATGTTAC TGCAAAACCC
    ATCCTTCAGC TTCTTAAGAA TCAAGAGTTT GTGAATATTA GCGTTTTACT AAGAAAAGAA
    AACACATCAG AAGGGGTTCG AGAATGGTGG GTAGTGAATC AAAGCCATAA AGAGTTCATT
    AAGAAGGATG GCCTGGAACT ATGGGTTTTC AGTGACAAAG TCAGTCCTCC AAGTCTTGGA
    TTCTTGGCAG GCTATGGAAT AATGGGACTA TATGCCTCTG TAGTTCTCGT AATAGGAAAG
    TTTGTGCGTG AGTTTTTCAG CGGAATTTCG CACTCCATCA TGTTTGAGGA GCTTCCCAAC
    GTTGACCGTA TACTGAAGCT GTGCACAGAC ATTTTCCTTG TGAGAGAGAC TGGAGAGTTG
    GAACTGGAGG AAGACCTGTA TGCCAAATTA ATATTTTTGT ACCGCTCCCC AGAAACTATG
    ATCAAATGGA CAAGAGAGAA AGATAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012820815.2
    CDS632..9001
    Translation

    Target ORF information:

    RefSeq Version XM_012965361.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis piezo-type mechanosensitive ion channel component 2 (piezo2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012965361.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGGCATCGG AAGTGGTGTG TGGGCTCATC TTCAGATTTC TTCTCCCACT ATGCCTGGTT 
    GTAGCGTGCA TGTTCCGGTA CAATGGAATA TCTTTTGTCT ACCTCATCTA CCTCTTGCTT
    ATACCTTTAT TTTCGGAGCC TACCAAAATA ACAATGCAAG GTCATACAGG ACGGTTATTA
    AAATCCTTAT GTGTTTCCAG TCTGTCATTT CTGCTGCTGC ACATCATCTT TCAAATTACC
    GTATACAGCC TCGAGGCCAG AAAACACATT GAACCACATT TTAACTGTTC AACATGGGAG
    AAAGCAATAC GACAAATTGG TTTTGAAAGT GTAAAAGGAG CAGATGCTGG TAATGGAATC
    CGCGTGTTTG CACCAGATAT CGGAATGTTT ATTGCAAGCT TGGCTACCCT GTTGGTTTGT
    AGAAAACTAG TTCAGAAAAC AGTTACTGAA GAGGACGCAC AATACAACCC CCAGTTTGTA
    AATGAAGAAA TGGCAGAAGA AGGAAAGGTG GACCTGGATG AAGCGTTAAT GTATGAGGAA
    GACTTTGATG GAGAAGAGGA TGAAATTGAG GCAGCTGAAG AAAGCACAAA ATTAAAAATT
    CTTCGCAGAA TTGCTTCATT TGCATCCGTA CTTAAAGAGT TTATTGGCAA CATTATAACC
    ACAGCTGGGA AAGTAGTTAT TACAATTTTA CTTGGTTTAA CAGGCATGAT GTTGCCGTCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT TTGGGGCTAT GCACATGGTG GTCCTGTTGC
    CGAGTATTTG ATCCGCTGAT ATTCAGCTGT CTATGTGTAT TAATGGCTAT ATTTAGTGCA
    GGGCACTTAA TTGGACTTTA TCTGTACCAG TTGCAGTTTT TTCACGAGAT TATTCCACCA
    AATGATTTCT ATGCCAGGTT GTTTGGTGTT ACTTCATTAG TTCAAACCGA CTGCTGTAAC
    ACTTGGAGAT TTTTTGCAAA CAACAGTCTA CCATGGAACC AGTATACCAA TCCTGTCATG
    CTTTTAGTAC TGTATTACAC GCTGGCAACT CTCATACGCC TTTGGCTACA GGAACCTATT
    AATGTAGAGG AAGAGAAGGA GGAAGACTGT AGCCCAGCAG CACTTACTGC AGAAAGAAGA
    CGTAGTTTGT GGTACTCTAG GCACTACACA ACGGATGAAA GACAACTTCT GTCCATGACT
    CAAGATGACT ACAAGCCATC TGATGTTTTA CTAGTAACTG TAAATGGGAC TCCCGTAGAT
    TACTGTTCAC TAAATTCATC ACTTCCGATA GAAAATGGTC CAACTAAAAT TGACATGTAT
    TCAACACCGC AATATAAATG GGACCATGAA GATGAAACTT CAGAAAAGAA AGAAGAGGAA
    GAGCAAGAAG AAGAACCCAA TGAAGAGGTT GCAGAAGAGA AAGAAAACAC TAAAGTTCAT
    GTAATGGTGG TGGCATTTCA GTTCATTATG AAGCAAAGCT ATATATGTGC ACTAATTTCA
    ATGATGGCCT GGAGCATCAC ATACCACAGC TGGTTGACTT TTGTTTTGTT AATCTGGTCT
    TGTGCTCTCT GGATGGTCCG AAATAGGAGA AAATATGCTA TGCTTAGTTC ACCTTTTATG
    GTTATGTATG GTAATTTACT TTTAACATTA CAATACATAT GGAGCATAGC CTTACAAAGT
    GATGAACTTC CAGAAGTTAT GGGTCTCCTT GAGAGAAAAA AGCCCGAAGA GCTTGTATCG
    AAGATCATTT TCACAGTTAC ATTTTGGCTT TTGCTCAGAC AACACCTTAC AGAGCAAAAG
    GCATTAAAGC AAAAAGAATT AGCATTATCT GAAATAAAAG TTGGAAGTGA AGACAAAGAA
    GGTGAAGTGC AAGAAGAAGA AGAAGAAGTG GAGGATGAAA ATGATATCAT GAAGGTCCTC
    GGAAAGCTAG TGCAGGCTAT GTTTGTCAAG TACTGGATTT ATGTCTGTGG CGGCATGTTT
    TTTTTTGTCA GCTTTGAAGG AAAAATAGTT ATGTACAAAA TTATCTACAT GATGCTCTTC
    CTATTCTGTG TAGCTCTGTA TCAGGTGCAC TATGAATGGT GGAGACGAAT TTTAAAATAT
    TTTTGGATGT CTGTGGTTGT TTACACCATG TTGGTGCTTG TTTTTATTTA CACTTACCAA
    TTTAAATCAT TTCCGGGCCT TTGGATGAAT ATGACAGGGC TGGATAAGGA AAAGTTAGCA
    GATCTTGGTT TAGAGACATT TAACTTGACT GACCTATTTA CTCGTATATT TATCCCAACC
    TCATTTTTGT TAGTGTGCAT TTTGCACCTA CATTACTTCC ATGACCGTTT TCTCCAGCTC
    ACTGACCTAA AGGCTGTAAC CAGCAAGCAG GACAGCACAA TTTACAGCCA TGCCAAAGTA
    AATGGTCGAG TTTACTTAAT AATAAATAGC ATCAAGCAAA AAATTCCAAT TCAGCAAAAT
    GAATTGGTGC ATCCAGAAGG AAGTTTGCCT GATATAACGA TGATGAACTT AACAACAGAA
    TCTGAAATGC TTGAGGAAAA GCGGTTGGAA ATTTATGACG AAAAAAAAAC TCATGACCAA
    GACTCAGATG ATGAATGGGA TAAAGGGGAT AGCAAAGAAC CAGGTCATTC TGATGATGAA
    GCTGAGGATG AGGAATCAGA AGAAGAAGAA ACAACAACAG AGTCCAGGAA CAAGTGGCAT
    CTAGTGATAG ATCGCTTAAC GGTTCTTTTC CTAAAGTTTC TGGAATATTT TCACAAGTTT
    CAGGTCTTTG TTTGGTGGCT TCTGGAGCTT CACATGATAA AGATTGTTTC TTCATACATC
    ATTTGGCTTA CTATAAAGGA GGTTTCACTG CTCAATTATG TATTTTTTAT TGCCTGGGCC
    TTCGCTTTGC CCTACTCACA ATTCCGGCCA TTGGCATCAA GTGTTTGCAC AGTCTGGACA
    TGTGTCATCA TCATATGTAA AATGTTGTAC CAGCTAGCCT CCATTGACCC TGCAACCTTT
    TCCAGCAACT GTTTAATGCC ACAGCTAAAC GAAACAACTG CACGTAACCA AACTGAGCTT
    AATTCTTCTG TGCTTTACAA TTCCTCTGTT GATCCTTCTA ATTGGGTTGG ACTAAAGAAG
    TCAACTCCAC TCCTTGCTTA TTTGCGGAAC AATTTGCTGA TGTTGGCCAT GTTAGCTTTT
    GAAGTAACCA TTTACCGGCA TCAGGAGTAT TACAGGTTGC GAAACAATCT CACCACACCT
    GCAACAAAAA CCATCTTTCA TGACATCACA AGACTTCACT TGGATGATGG ACTCATAAAT
    AGTGCCAAGT ATTTTGTGAA CTACTTTTTC TACAAGTTTG GATTGGAAAC ATGTTTCTTA
    CTGTCGGTAA ATGTAATCGG TCAGAGGATG GACTTTTTTG CCATGGTTCA TGCACTTTGG
    CTTTTTGGCG TATTATACCG ACGCAGAAGG AAAGCGATTG CAGAAACCTG GCACAGATAT
    TGCTGTTTTC TTGCTTGCAT TATGGCATTC CAGTATTTTA TATGTATTGG CATCCCCCCT
    GCTGCATGTA AAGACTATCC TTGGAGACAA CCTGGAGCCA ATTTTAATTC TAACATAATA
    AAGTGGTTAT ACTTTCCAGA CTTTATTGTG AGGCCTAGTC CTCTGTTTCT TGTCTATGAT
    TTTTTGCTTT TGCTTTGTGC GTCCCTTCAA AGAGAAGTGT TTGAGGAAGA AAATAAGGCA
    GCAGTTCGAA TCATGGCTGG TGACAATGTA GAAATTTGCA TGAACCTTGA TGCTGCATCT
    TTTAGCCAAC ATAATCCTGT ACCTGATTTC ATATACTGCA GATCTTATTT GGACATGGGT
    AAAGTTATAA TGTTTAGTTA CCTTTTCTGG TTTGTGCTCA CCATCATCTT CATCACGGGA
    ACTACACGGA TCAGCATATT CTGCATGGGC TACTTGGTAG CTTGCTTCTA TTTTCTATTA
    TTTGGTGGAG ATTTGCTGTT AAAACCTATA AGAATCATCC TACGCTATTG GGATTGGCTG
    ATTGCTTACA ATGTTTTTGT CATTACAATG AAAAATGTAT TATCAATAGG AGCATGTGGC
    TACATTGAAT ATTTAATCAA AGACAGTTGT TGGTTAATTC AGGCATTCAG TCTTTCTTGT
    ACAGTGAAAG GTTATTTTAT CCCCCAAAAC ACACCTGACT GTCAGTTGCC AAGCGGAGAG
    GCTGGAATCA TTTGGGACAG TATATGTTTT GCTTTCCTTT TATTGCAAAG AAGAGTGTTT
    ATGAGCTATT ACTTCTTACA TGTGGTTGCA GACATCAAAT CATCTCAGAT TCTAGCATCA
    AGAGGTGCTG AACTGTTCCA AGCTACAATT GTAAAAGCTG TAAAAGCAAG AATTGAAGAA
    GAAAAAAAAT CTATGGACCA GCTCAAGAGA CAGATGGACC ACATTAAAGC CAGGCAAGAA
    AAATATAAAA AAGGTAAAGA AAAAATGCTG AGTCTTACAC AGGATGGAGA AGGACAAGAA
    GTTCAAAAAC TAGATGATGA CGATGAAGAA GGAGACAAAG CAGACAAAGA GAAAGCCAAG
    GGCAAAAAAA AGCAGTGGTG GCGACCTTGG GTTGATCATG CTTCCATGGT GAAAAGCGGT
    GATTATTATT TGTTTGAGAC GGACAGTGAA GAAGAAGATG AGGATGACAA AAAAGAAGAA
    GAGCCTAGAA GAAAATCTGC CTTCCAGTTT TTGTACCAAG CATGGATTAC TGATCCAAAA
    ACAGCCCTTA AGCAAAGAAG CATGGAAGCC AAAGAAGAAA AGGCAAGGGA AATGAGGCGA
    AAGGGCATTG CTGAAGGTGA TATTATTGTA GAATGTGGGG ATAAAGAAGA AGAAAAAGAG
    CAAGGAAAAA AGAAATCAGA TGGCCCAGAC AATATCTTCA AAAGAATATT TAACATCCTG
    AAGTTTACTT GGGTCCTCTT CCTTGCTACA ATCGATAGTT TTACGGCTTG GCTAAACTCC
    ATTTCAAGAG AACATATTGA TATCTCCAAA GTTCTAAGCA TAGAACGTTG CATGCTGACT
    AGAGAGATAA AAAAGGGCAA TGTGCCTACT CGAGAAAGCA TCCACATGTA TTACCAAAAT
    CAAATAATGA TGAGCCTTTC TAGAGAGTCT GGACTTGACA GAATTGATGA CCAACCTGGT
    TCATCGTCCA GTCACCATAG CCATGACAAG ATGGCGAGTA AAAGGATGGA CAGTGCAGAC
    TCAGCTGTAT CTCATGATAG CATATCAAGC TGCTGCACTG AAGCAACCAT GCTATTCTCA
    AGGCAATCTA CACTTGATGA TTTAGATGGA CCAGACTCTG TACCAAAAAC AAGTGAGCGT
    GCTCGACCTA GGCTTCGTAA AATGTACAGC ATGGACATGT CTTCTTCATC TGCTGACAGT
    ATAGCTTCAA GTGAGCCAAC TCAGTGCACC ATGGTCTACT CACGGCAGGG CACAACAGAA
    ACTATTGAGG AAGTGGAAGA TGAAGAGGAG GAAGAAAGCA AAAGTCCTTT ATCAAAGATA
    GAAGCCGAGG AAGAAGAATA TGACATGGAT TTGGAAGGGA CAAGCTATAC TCCGGACACA
    GAATTTCCCC TGTACGCCAC TACAGAGGGG GATCTTCCTC CTTCGTACAG TAAAGCCGGC
    AGCTTCGAAC AACTTACATT TGTATCTCAG GACTCGGCTG ACAATTTAAT GGTTCTAAGC
    CCAGATGAAA GTCGTACTGA TAAGCTACAT GATCATATTC TTCCACCTCT TACACATGAA
    CTGACTGCTA GCGAGCTGCT TCTAAACAAA ATGTTTTATG ATGAAGAGTT AGAAGACTCT
    GAAAAATTTT ATACTACTCA ACCACGCCCG CTGCAGCTTA TTTATGCCTT GTATAATACA
    TTGGTGGCCA GATCAGAAAT GGTGTGCTAT TTTGTGATTA TTCTTAATCA CATGATTTCA
    GCTTCTATGA TCACATTGGT ACTGCCCATC CTTATATTCC TTTGGGCTAT GCTTTCTGTC
    CCAAGGCCCA GTAAGAGATT TTGGATGACA GCTATTGTAT ATACAGAGGT GACAATTGTT
    ATTAAATATT TTTTCCAATT TGGATTCTTC CCATGGAATA AAAATGTGGA TTATACAAAG
    GACAAACCTT ACTTCCCACC TAACATAATG GGCATTGAGA AGAAAGAAGG TTATGTGCAC
    TATGACCTGG TGCAACTGCT TGCTCTGTTT TTCCATAGAT CTATTTTAAA GTGCCACGGG
    CTTTGGGATG AAGATGATGT CACTGAAAAA GCCACAGATA AAGACGACAC GGATGGAGAG
    ATATCAGAGG CATCAGGAAG AAGGGATTCC GTTGGCTCAT TGAAATCTGT CAATCTAGCT
    GCGTCTGTGG AGTCAGTCCA TGTGCACTTC CCTGAGGAAA AGACCGCTAT CAGGAGAAAA
    AGCACAAGCA GTGGATCGCA AATTTCCCGA AGGTCAAGTT ATTCCTCGCA CAGGTCTAAA
    AGAGGCAGCA CCAGTACTCG GAACAGCAGT CAAAAAGGAA GCAGCATTCC CAGTATTAAA
    CTGAAATCTA GAAAGGAACT TATACTGGAA AAACTCAGAG AACAGCTCAT TAAAGCAAAA
    GCATTTACGA TCAAGACGAT ACTACAAATC TATGTCCCGA TCAGACAGTT TTTTTACAAT
    CTTATACATC CTGAATACAG TGCTGTGACT GATGTCTATG TGCTGATGTT CCTGGCAGAC
    ACTGTAGATT TCATCATTAT TGTTTTTGGA TTTTGGGCGT TTGGAAAACA CTCCGCTGCA
    GCAGATATTA CATCCTCTCT ATCAGAAGAC CAAGTCCCAG AAGCATTTTT GGTAATGGTG
    CTTATCCAGT TTGGAACCAT GGTTGTTGAT CGTGCTTTGT ATCTTCGCAA AACAGTCATG
    GGAAAAGTTA TTTTCCAAGT TATCCTTGTA TTTGGAATCC ATTTTTGGAT GTTCTTCATC
    TTGCCAGGGG TAACAGAAAG GAAATTTAGT CAGAACACTG TTGCCCAACT GTGGTATTTT
    GTCAAGTGCA TTTATTTTGG ATTGTCGGCA TATCAGATAC GTTGTGGATA TCCAACTCGG
    GTGCTTGGTA ACTTCCTTAC TAAAAGCTAC AATTATGTCA ACCTGTTCCT TTTCCAAGGC
    TTTCGCCTCG TTCCATTTCT TACGGAGCTG CGAGCTGTTA TGGACTGGGT ATGGACTGAC
    ACCACTCTCA GTCTTTCCAG CTGGATCTGT GTAGAGGACA TTTATGCACA TATATTTATA
    CTAAAATGCT GGCGGGAGTC AGAAAAGAGA TATCCCCAGC CACGAGGCCA AAAGAAGAAA
    AAGGTTGTGA AATACGGAAT GGGTGGAATG ATAATAGTTC TGCTGATTTG TATCGTGTGG
    TTTCCTTTGC TCTTCATGTC CTTGATTAAA TCAGTTGCTG GGGTCACTAA CAAGCCCCTA
    GATGTGTCCA TTACAATAAC CCTTGGAGGT TATCAGCCTA TTTTTACCAT GAGTGCACAA
    CAAAATCAGC TGCAAGGCTT GAATGACAAT GAATTTAAGC ATTTACATAA TATATATAAA
    GGCAATACAC CTGCCATGCA GTTTCTGGAA TCCTATATGC AGGAAGACAT CACCATAGCA
    GGACTGGAAG GCAATTCCAA CTCTCTGTGG ACCATCAGCC CTCCGAGCAG GACAATGATG
    ATAGAAAGAC TTATCCAGGA ACCTGACTTC ACAGCGGTCA TTTCTTGGTC CATTCGAAGA
    AATGTGAGTC TTGGAGCTAA GGCAGAGGTA ACAGGTGAAA AATATACAAT ATCACTGGCA
    GGTCAAACTC GGAGGGAACT TGCGGATATG ATGAATAGTT CATCTCCCAA TAAGACAGTG
    GAACTGATAA AGATATACCC ATTGTGTTTA AAAGCACCAA GTGATGTTAC TGCAAAACCC
    ATCCTTCAGC TTCTTAAGAA TCAAGAGTTT GTGAATATTA GCGTTTTACT AAGAAAAGAA
    AACACATCAG AAGGGGTTCG AGAATGGTGG GTAGTGAATC AAAGCCATAA AGAGTTCATT
    AAGAAGGATG GCCTGGAACT ATGGGTTTTC AGTGACAAAG TCAGTCCTCC AAGTCTTGGA
    TTCTTGGCAG GCTATGGAAT AATGGGACTA TATGCCTCTG TAGTTCTCGT AATAGGAAAG
    TTTGTGCGTG AGTTTTTCAG CGGAATTTCG CACTCCATCA TGTTTGAGGA GCTTCCCAAC
    GTTGACCGTA TACTGAAGCT GTGCACAGAC ATTTTCCTTG TGAGAGAGAC TGGAGAGTTG
    GAACTGGAGG AAGACCTGTA TGCCAAATTA ATATTTTTGT ACCGCTCCCC AGAAACTATG
    ATCAAATGGA CAAGAGAGAA AGATAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa60021
    Clone ID Related Accession (Same CDS sequence) XM_012965362.3
    Accession Version XM_012965362.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8307bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product piezo-type mechanosensitive ion channel component 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012965362.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    ATGGCATCGG AAGTGGTGTG TGGGCTCATC TTCAGATTTC TTCTCCCACT ATGCCTGGTT 
    GTAGCGTGCA TGTTCCGGTA CAATGGAATA TCTTTTGTCT ACCTCATCTA CCTCTTGCTT
    ATACCTTTAT TTTCGGAGCC TACCAAAATA ACAATGCAAG GTCATACAGG ACGGTTATTA
    AAATCCTTAT GTGTTTCCAG TCTGTCATTT CTGCTGCTGC ACATCATCTT TCAAATTACC
    GTATACAGCC TCGAGGCCAG AAAACACATT GAACCACATT TTAACTGTTC AACATGGGAG
    AAAGCAATAC GACAAATTGG TTTTGAAAGT GTAAAAGGAG CAGATGCTGG TAATGGAATC
    CGCGTGTTTG CACCAGATAT CGGAATGTTT ATTGCAAGCT TGGCTACCCT GTTGGTTTGT
    AGAAAACTAG TTCAGAAAAC AGTTACTGAA GAGGACGCAC AATACAACCC CCAGTTTGTA
    AATGAAGAAA TGGCAGAAGA AGGAAAGGTG GACCTGGATG AAGCGTTAAT GTATGAGGAA
    GACTTTGATG GAGAAGAGGA TGAAATTGAG GCAGCTGAAG AAAGCACAAA ATTAAAAATT
    CTTCGCAGAA TTGCTTCATT TGCATCCGTA CTTAAAGAGT TTATTGGCAA CATTATAACC
    ACAGCTGGGA AAGTAGTTAT TACAATTTTA CTTGGTTTAA CAGGCATGAT GTTGCCGTCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT TTGGGGCTAT GCACATGGTG GTCCTGTTGC
    CGAGTATTTG ATCCGCTGAT ATTCAGCTGT CTATGTGTAT TAATGGCTAT ATTTAGTGCA
    GGGCACTTAA TTGGACTTTA TCTGTACCAG TTGCAGTTTT TTCACGAGAT TATTCCACCA
    AATGATTTCT ATGCCAGGTT GTTTGGTGTT ACTTCATTAG TTCAAACCGA CTGCTGTAAC
    ACTTGGAGAT TTTTTGCAAA CAACAGTCTA CCATGGAACC AGTATACCAA TCCTGTCATG
    CTTTTAGTAC TGTATTACAC GCTGGCAACT CTCATACGCC TTTGGCTACA GGAACCTATT
    AATGTAGAGG AAGAGAAGGA GGAAGACTGT AGCCCAGCAG CACTTACTGC AGAAAGAAGA
    CGTAGTTTGT GGTACTCTAG GCACTACACA ACGGATGAAA GACAACTTCT GTCCATGACT
    CAAGATGACT ACAAGCCATC TGATGTTTTA CTAGTAACTG TAAATGGGAC TCCCGTAGAT
    TACTGTTCAC TAAATTCATC ACTTCCGATA GAAAATGGTC CAACTAAAAT TGACATGTAT
    TCAACACCGC AATATAAATG GGACCATGAA GATGAAACTT CAGAAAAGAA AGAAGAGGAA
    GAGCAAGAAG AAGAACCCAA TGAAGAGGTT GCAGAAGAGA AAGAAAACAC TAAAGTTCAT
    GTAATGGTGG TGGCATTTCA GTTCATTATG AAGCAAAGCT ATATATGTGC ACTAATTTCA
    ATGATGGCCT GGAGCATCAC ATACCACAGC TGGTTGACTT TTGTTTTGTT AATCTGGTCT
    TGTGCTCTCT GGATGGTCCG AAATAGGAGA AAATATGCTA TGCTTAGTTC ACCTTTTATG
    GTTATGTATG GTAATTTACT TTTAACATTA CAATACATAT GGAGCATAGC CTTACAAAGT
    GATGAACTTC CAGAAGTTAT GGGTCTCCTT GAGAGAAAAA AGCCCGAAGA GCTTGTATCG
    AAGATCATTT TCACAGTTAC ATTTTGGCTT TTGCTCAGAC AACACCTTAC AGAGCAAAAG
    GCATTAAAGC AAAAAGAATT AGCATTATCT GAAATAAAAG TTGGAAGTGA AGACAAAGAA
    GGTGAAGTGC AAGAAGAAGA AGAAGAAGTG GAGGATGAAA ATGATATCAT GAAGGTCCTC
    GGAAAGCTAG TGCAGGCTAT GTTTGTCAAG TACTGGATTT ATGTCTGTGG CGGCATGTTT
    TTTTTTGTCA GCTTTGAAGG AAAAATAGTT ATGTACAAAA TTATCTACAT GATGCTCTTC
    CTATTCTGTG TAGCTCTGTA TCAGGTGCAC TATGAATGGT GGAGACGAAT TTTAAAATAT
    TTTTGGATGT CTGTGGTTGT TTACACCATG TTGGTGCTTG TTTTTATTTA CACTTACCAA
    TTTAAATCAT TTCCGGGCCT TTGGATGAAT ATGACAGGGC TGGATAAGGA AAAGTTAGCA
    GATCTTGGTT TAGAGACATT TAACTTGACT GACCTATTTA CTCGTATATT TATCCCAACC
    TCATTTTTGT TAGTGTGCAT TTTGCACCTA CATTACTTCC ATGACCGTTT TCTCCAGCTC
    ACTGACCTAA AGGCTGTAAC CAGCAAGCAG GACAGCACAA TTTACAGCCA TGCCAAAGTA
    AATGGTCGAG TTTACTTAAT AATAAATAGC ATCAAGCAAA AAATTCCAAT TCAGCAAAAT
    GAATTGGTGC ATCCAGAAGG AAGTTTGCCT GATATAACGA TGATGAACTT AACAACAGAA
    TCTGAAATGC TTGAGGAAAA GCGGTTGGAA ATTTATGACG AAAAAAAAAC TCATGACCAA
    GACTCAGATG ATGAATGGGA TAAAGGGGAT AGCAAAGAAC CAGGTCATTC TGATGATGAA
    GCTGAGGATG AGGAATCAGA AGAAGAAGAA ACAACAACAG AGTCCAGGAA CAAGTGGCAT
    CTAGTGATAG ATCGCTTAAC GGTTCTTTTC CTAAAGTTTC TGGAATATTT TCACAAGTTT
    CAGGTCTTTG TTTGGTGGCT TCTGGAGCTT CACATGATAA AGATTGTTTC TTCATACATC
    ATTTGGCTTA CTATAAAGGA GGTTTCACTG CTCAATTATG TATTTTTTAT TGCCTGGGCC
    TTCGCTTTGC CCTACTCACA ATTCCGGCCA TTGGCATCAA GTGTTTGCAC AGTCTGGACA
    TGTGTCATCA TCATATGTAA AATGTTGTAC CAGCTAGCCT CCATTGACCC TGCAACCTTT
    TCCAGCAACT GTTTAATGCC ACAGCTAAAC GAAACAACTG CACGTAACCA AACTGAGCTT
    AATTCTTCTG TGCTTTACAA TTCCTCTGTT GATCCTTCTA ATTGGGTTGG ACTAAAGAAG
    TCAACTCCAC TCCTTGCTTA TTTGCGGAAC AATTTGCTGA TGTTGGCCAT GTTAGCTTTT
    GAAGTAACCA TTTACCGGCA TCAGGAGTAT TACAGGTTGC GAAACAATCT CACCACACCT
    GCAACAAAAA CCATCTTTCA TGACATCACA AGACTTCACT TGGATGATGG ACTCATAAAT
    AGTGCCAAGT ATTTTGTGAA CTACTTTTTC TACAAGTTTG GATTGGAAAC ATGTTTCTTA
    CTGTCGGTAA ATGTAATCGG TCAGAGGATG GACTTTTTTG CCATGGTTCA TGCACTTTGG
    CTTTTTGGCG TATTATACCG ACGCAGAAGG AAAGCGATTG CAGAAACCTG GCACAGATAT
    TGCTGTTTTC TTGCTTGCAT TATGGCATTC CAGTATTTTA TATGTATTGG CATCCCCCCT
    GCTGCATGTA AAGACTATCC TTGGAGACAA CCTGGAGCCA ATTTTAATTC TAACATAATA
    AAGTGGTTAT ACTTTCCAGA CTTTATTGTG AGGCCTAGTC CTCTGTTTCT TGTCTATGAT
    TTTTTGCTTT TGCTTTGTGC GTCCCTTCAA AGAGAAGTGT TTGAGGAAGA AAATAAGGCA
    GCAGTTCGAA TCATGGCTGG TGACAATGTA GAAATTTGCA TGAACCTTGA TGCTGCATCT
    TTTAGCCAAC ATAATCCTGT ACCTGATTTC ATATACTGCA GATCTTATTT GGACATGGGT
    AAAGTTATAA TGTTTAGTTA CCTTTTCTGG TTTGTGCTCA CCATCATCTT CATCACGGGA
    ACTACACGGA TCAGCATATT CTGCATGGGC TACTTGGTAG CTTGCTTCTA TTTTCTATTA
    TTTGGTGGAG ATTTGCTGTT AAAACCTATA AGAATCATCC TACGCTATTG GGATTGGCTG
    ATTGCTTACA ATGTTTTTGT CATTACAATG AAAAATGTAT TATCAATAGG AGCATGTGGC
    TACATTGAAT ATTTAATCAA AGACAGTTGT TGGTTAATTC AGGCATTCAG TCTTTCTTGT
    ACAGTGAAAG GTTATTTTAT CCCCCAAAAC ACACCTGACT GTCAGTTGCC AAGCGGAGAG
    GCTGGAATCA TTTGGGACAG TATATGTTTT GCTTTCCTTT TATTGCAAAG AAGAGTGTTT
    ATGAGCTATT ACTTCTTACA TGTGGTTGCA GACATCAAAT CATCTCAGAT TCTAGCATCA
    AGAGGTGCTG AACTGTTCCA AGCTACAATT GTAAAAGCTG TAAAAGCAAG AATTGAAGAA
    GAAAAAAAAT CTATGGACCA GCTCAAGAGA CAGATGGACC ACATTAAAGC CAGGCAAGAA
    AAATATAAAA AAGGTAAAGA AAAAATGCTG AGTCTTACAC AGGATGGAGA AGGACAAGAA
    GTTCAAAAAC TAGATGATGA CGATGAAGAA GGAGACAAAG CAGACAAAGA GAAAGCCAAG
    GGCAAAAAAA AGCAGTGGTG GCGACCTTGG GTTGATCATG CTTCCATGGT GAAAAGCGGT
    GATTATTATT TGTTTGAGAC GGACAGTGAA GAAGAAGATG AGGATGACAA AAAAGAAGAA
    GAGCCTAGAA GAAAATCTGC CTTCCAGAGA GCTATTGGAA AGTTTGCATC AGCCATTATA
    GCCTTGCCAA AGTCTGTCAT TAAACTGCCT AAAACAATTC TTCAATATTT AATCAGAGCT
    GCAAAGTTTT TGTACCAAGC ATGGATTACT GATCCAAAAA CAGCCCTTAA GCAAAGAAGC
    ATGGAAGCCA AAGAAGAAAA GGCAAGGGAA ATGAGGCGAA AGGGCATTGC TGAAGGTGAT
    ATTATTGTAG AATGTGGGGA TAAAGAAGAA GAAAAAGAGC AAGGAAAAAA GAAATCAGAT
    GGCCCAGACA ATATCTTCAA AAGAATATTT AACATCCTGA AGTTTACTTG GGTCCTCTTC
    CTTGCTACAA TCGATAGTTT TACGGCTTGG CTAAACTCCA TTTCAAGAGA ACATATTGAT
    ATCTCCAAAG TTCTAAGCAT AGAACGTTGC ATGCTGACTA GAGAGATAAA AAAGGGCAAT
    GTGCCTACTC GAGAAAGCAT CCACATGTAT TACCAAAATC AAATAATGAT GAGCCTTTCT
    AGAGAGTCTG GACTTGACAG AATTGATGAC CAACCTGGTT CATCGTCCAG TCACCATAGC
    CATGACAAGA TGGCGAGTAA AAGGATGGAC AGTGCAGACT CAGCTGTATC TCATGATAGC
    ATATCAAGTG AGCCAACTCA GTGCACCATG GTCTACTCAC GGCAGGGCAC AACAGAAACT
    ATTGAGGAAG TGGAAGATGA AGAGGAGGAA GAAAGCAAAA GTCCTTTATC AAAGATAGAA
    GCCGAGGAAG AAGAATATGA CATGGATTTG GAAGGGACAA GCTATACTCC GGACACAGAA
    TTTCCCCTGT ACGCCACTAC AGAGGGGGAT CTTCCTCCTT CGTACAGTAA AGCCGGCAGC
    TTCGAACAAC TTACATTTGT ATCTCAGGAC TCGGCTGACA ATTTAATGGT TCTAAGCCCA
    GATGAAAGTC GTACTGATAA GCTACATGAT CATATTCTTC CACCTCTTAC ACATGAACTG
    ACTGCTAGCG AGCTGCTTCT AAACAAAATG TTTTATGATG AAGAGTTAGA AGACTCTGAA
    AAATTTTATA CTACTCAACC ACGCCCGCTG CAGCTTATTT ATGCCTTGTA TAATACATTG
    GTGGCCAGAT CAGAAATGGT GTGCTATTTT GTGATTATTC TTAATCACAT GATTTCAGCT
    TCTATGATCA CATTGGTACT GCCCATCCTT ATATTCCTTT GGGCTATGCT TTCTGTCCCA
    AGGCCCAGTA AGAGATTTTG GATGACAGCT ATTGTATATA CAGAGGTGAC AATTGTTATT
    AAATATTTTT TCCAATTTGG ATTCTTCCCA TGGAATAAAA ATGTGGATTA TACAAAGGAC
    AAACCTTACT TCCCACCTAA CATAATGGGC ATTGAGAAGA AAGAAGGTTA TGTGCACTAT
    GACCTGGTGC AACTGCTTGC TCTGTTTTTC CATAGATCTA TTTTAAAGTG CCACGGGCTT
    TGGGATGAAG ATGATGTCAC TGAAAAAGCC ACAGATAAAG ACGACACGGA TGGAGAGATA
    TCAGAGGCAT CAGGAAGAAG GGATTCCGTT GGCTCATTGA AATCTGTCAA TCTAGCTGCG
    TCTGTGGAGT CAGTCCATGT GCACTTCCCT GAGGAAAAGA CCGCTATCAG GAGAAAAAGC
    ACAAGCAGTG GATCGCAAAT TTCCCGAAGG TCAAGTTATT CCTCGCACAG GTCTAAAAGA
    GGCAGCACCA GTACTCGGAA CAGCAGTCAA AAAGGAAGCA GCATTCCCAG TATTAAACTG
    AAATCTAGAA AGGAACTTAT ACTGGAAAAA CTCAGAGAAC AGCTCATTAA AGCAAAAGCA
    TTTACGATCA AGACGATACT ACAAATCTAT GTCCCGATCA GACAGTTTTT TTACAATCTT
    ATACATCCTG AATACAGTGC TGTGACTGAT GTCTATGTGC TGATGTTCCT GGCAGACACT
    GTAGATTTCA TCATTATTGT TTTTGGATTT TGGGCGTTTG GAAAACACTC CGCTGCAGCA
    GATATTACAT CCTCTCTATC AGAAGACCAA GTCCCAGAAG CATTTTTGGT AATGGTGCTT
    ATCCAGTTTG GAACCATGGT TGTTGATCGT GCTTTGTATC TTCGCAAAAC AGTCATGGGA
    AAAGTTATTT TCCAAGTTAT CCTTGTATTT GGAATCCATT TTTGGATGTT CTTCATCTTG
    CCAGGGGTAA CAGAAAGGAA ATTTAGTCAG AACACTGTTG CCCAACTGTG GTATTTTGTC
    AAGTGCATTT ATTTTGGATT GTCGGCATAT CAGATACGTT GTGGATATCC AACTCGGGTG
    CTTGGTAACT TCCTTACTAA AAGCTACAAT TATGTCAACC TGTTCCTTTT CCAAGGCTTT
    CGCCTCGTTC CATTTCTTAC GGAGCTGCGA GCTGTTATGG ACTGGGTATG GACTGACACC
    ACTCTCAGTC TTTCCAGCTG GATCTGTGTA GAGGACATTT ATGCACATAT ATTTATACTA
    AAATGCTGGC GGGAGTCAGA AAAGAGATAT CCCCAGCCAC GAGGCCAAAA GAAGAAAAAG
    GTTGTGAAAT ACGGAATGGG TGGAATGATA ATAGTTCTGC TGATTTGTAT CGTGTGGTTT
    CCTTTGCTCT TCATGTCCTT GATTAAATCA GTTGCTGGGG TCACTAACAA GCCCCTAGAT
    GTGTCCATTA CAATAACCCT TGGAGGTTAT CAGCCTATTT TTACCATGAG TGCACAACAA
    AATCAGCTGC AAGGCTTGAA TGACAATGAA TTTAAGCATT TACATAATAT ATATAAAGGC
    AATACACCTG CCATGCAGTT TCTGGAATCC TATATGCAGG AAGACATCAC CATAGCAGGA
    CTGGAAGGCA ATTCCAACTC TCTGTGGACC ATCAGCCCTC CGAGCAGGAC AATGATGATA
    GAAAGACTTA TCCAGGAACC TGACTTCACA GCGGTCATTT CTTGGTCCAT TCGAAGAAAT
    GTGAGTCTTG GAGCTAAGGC AGAGGTAACA GGTGAAAAAT ATACAATATC ACTGGCAGGT
    CAAACTCGGA GGGAACTTGC GGATATGATG AATAGTTCAT CTCCCAATAA GACAGTGGAA
    CTGATAAAGA TATACCCATT GTGTTTAAAA GCACCAAGTG ATGTTACTGC AAAACCCATC
    CTTCAGCTTC TTAAGAATCA AGAGTTTGTG AATATTAGCG TTTTACTAAG AAAAGAAAAC
    ACATCAGAAG GGGTTCGAGA ATGGTGGGTA GTGAATCAAA GCCATAAAGA GTTCATTAAG
    AAGGATGGCC TGGAACTATG GGTTTTCAGT GACAAAGTCA GTCCTCCAAG TCTTGGATTC
    TTGGCAGGCT ATGGAATAAT GGGACTATAT GCCTCTGTAG TTCTCGTAAT AGGAAAGTTT
    GTGCGTGAGT TTTTCAGCGG AATTTCGCAC TCCATCATGT TTGAGGAGCT TCCCAACGTT
    GACCGTATAC TGAAGCTGTG CACAGACATT TTCCTTGTGA GAGAGACTGG AGAGTTGGAA
    CTGGAGGAAG ACCTGTATGC CAAATTAATA TTTTTGTACC GCTCCCCAGA AACTATGATC
    AAATGGACAA GAGAGAAAGA TAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012820816.2
    CDS632..8938
    Translation

    Target ORF information:

    RefSeq Version XM_012965362.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis piezo-type mechanosensitive ion channel component 2 (piezo2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012965362.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    ATGGCATCGG AAGTGGTGTG TGGGCTCATC TTCAGATTTC TTCTCCCACT ATGCCTGGTT 
    GTAGCGTGCA TGTTCCGGTA CAATGGAATA TCTTTTGTCT ACCTCATCTA CCTCTTGCTT
    ATACCTTTAT TTTCGGAGCC TACCAAAATA ACAATGCAAG GTCATACAGG ACGGTTATTA
    AAATCCTTAT GTGTTTCCAG TCTGTCATTT CTGCTGCTGC ACATCATCTT TCAAATTACC
    GTATACAGCC TCGAGGCCAG AAAACACATT GAACCACATT TTAACTGTTC AACATGGGAG
    AAAGCAATAC GACAAATTGG TTTTGAAAGT GTAAAAGGAG CAGATGCTGG TAATGGAATC
    CGCGTGTTTG CACCAGATAT CGGAATGTTT ATTGCAAGCT TGGCTACCCT GTTGGTTTGT
    AGAAAACTAG TTCAGAAAAC AGTTACTGAA GAGGACGCAC AATACAACCC CCAGTTTGTA
    AATGAAGAAA TGGCAGAAGA AGGAAAGGTG GACCTGGATG AAGCGTTAAT GTATGAGGAA
    GACTTTGATG GAGAAGAGGA TGAAATTGAG GCAGCTGAAG AAAGCACAAA ATTAAAAATT
    CTTCGCAGAA TTGCTTCATT TGCATCCGTA CTTAAAGAGT TTATTGGCAA CATTATAACC
    ACAGCTGGGA AAGTAGTTAT TACAATTTTA CTTGGTTTAA CAGGCATGAT GTTGCCGTCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT TTGGGGCTAT GCACATGGTG GTCCTGTTGC
    CGAGTATTTG ATCCGCTGAT ATTCAGCTGT CTATGTGTAT TAATGGCTAT ATTTAGTGCA
    GGGCACTTAA TTGGACTTTA TCTGTACCAG TTGCAGTTTT TTCACGAGAT TATTCCACCA
    AATGATTTCT ATGCCAGGTT GTTTGGTGTT ACTTCATTAG TTCAAACCGA CTGCTGTAAC
    ACTTGGAGAT TTTTTGCAAA CAACAGTCTA CCATGGAACC AGTATACCAA TCCTGTCATG
    CTTTTAGTAC TGTATTACAC GCTGGCAACT CTCATACGCC TTTGGCTACA GGAACCTATT
    AATGTAGAGG AAGAGAAGGA GGAAGACTGT AGCCCAGCAG CACTTACTGC AGAAAGAAGA
    CGTAGTTTGT GGTACTCTAG GCACTACACA ACGGATGAAA GACAACTTCT GTCCATGACT
    CAAGATGACT ACAAGCCATC TGATGTTTTA CTAGTAACTG TAAATGGGAC TCCCGTAGAT
    TACTGTTCAC TAAATTCATC ACTTCCGATA GAAAATGGTC CAACTAAAAT TGACATGTAT
    TCAACACCGC AATATAAATG GGACCATGAA GATGAAACTT CAGAAAAGAA AGAAGAGGAA
    GAGCAAGAAG AAGAACCCAA TGAAGAGGTT GCAGAAGAGA AAGAAAACAC TAAAGTTCAT
    GTAATGGTGG TGGCATTTCA GTTCATTATG AAGCAAAGCT ATATATGTGC ACTAATTTCA
    ATGATGGCCT GGAGCATCAC ATACCACAGC TGGTTGACTT TTGTTTTGTT AATCTGGTCT
    TGTGCTCTCT GGATGGTCCG AAATAGGAGA AAATATGCTA TGCTTAGTTC ACCTTTTATG
    GTTATGTATG GTAATTTACT TTTAACATTA CAATACATAT GGAGCATAGC CTTACAAAGT
    GATGAACTTC CAGAAGTTAT GGGTCTCCTT GAGAGAAAAA AGCCCGAAGA GCTTGTATCG
    AAGATCATTT TCACAGTTAC ATTTTGGCTT TTGCTCAGAC AACACCTTAC AGAGCAAAAG
    GCATTAAAGC AAAAAGAATT AGCATTATCT GAAATAAAAG TTGGAAGTGA AGACAAAGAA
    GGTGAAGTGC AAGAAGAAGA AGAAGAAGTG GAGGATGAAA ATGATATCAT GAAGGTCCTC
    GGAAAGCTAG TGCAGGCTAT GTTTGTCAAG TACTGGATTT ATGTCTGTGG CGGCATGTTT
    TTTTTTGTCA GCTTTGAAGG AAAAATAGTT ATGTACAAAA TTATCTACAT GATGCTCTTC
    CTATTCTGTG TAGCTCTGTA TCAGGTGCAC TATGAATGGT GGAGACGAAT TTTAAAATAT
    TTTTGGATGT CTGTGGTTGT TTACACCATG TTGGTGCTTG TTTTTATTTA CACTTACCAA
    TTTAAATCAT TTCCGGGCCT TTGGATGAAT ATGACAGGGC TGGATAAGGA AAAGTTAGCA
    GATCTTGGTT TAGAGACATT TAACTTGACT GACCTATTTA CTCGTATATT TATCCCAACC
    TCATTTTTGT TAGTGTGCAT TTTGCACCTA CATTACTTCC ATGACCGTTT TCTCCAGCTC
    ACTGACCTAA AGGCTGTAAC CAGCAAGCAG GACAGCACAA TTTACAGCCA TGCCAAAGTA
    AATGGTCGAG TTTACTTAAT AATAAATAGC ATCAAGCAAA AAATTCCAAT TCAGCAAAAT
    GAATTGGTGC ATCCAGAAGG AAGTTTGCCT GATATAACGA TGATGAACTT AACAACAGAA
    TCTGAAATGC TTGAGGAAAA GCGGTTGGAA ATTTATGACG AAAAAAAAAC TCATGACCAA
    GACTCAGATG ATGAATGGGA TAAAGGGGAT AGCAAAGAAC CAGGTCATTC TGATGATGAA
    GCTGAGGATG AGGAATCAGA AGAAGAAGAA ACAACAACAG AGTCCAGGAA CAAGTGGCAT
    CTAGTGATAG ATCGCTTAAC GGTTCTTTTC CTAAAGTTTC TGGAATATTT TCACAAGTTT
    CAGGTCTTTG TTTGGTGGCT TCTGGAGCTT CACATGATAA AGATTGTTTC TTCATACATC
    ATTTGGCTTA CTATAAAGGA GGTTTCACTG CTCAATTATG TATTTTTTAT TGCCTGGGCC
    TTCGCTTTGC CCTACTCACA ATTCCGGCCA TTGGCATCAA GTGTTTGCAC AGTCTGGACA
    TGTGTCATCA TCATATGTAA AATGTTGTAC CAGCTAGCCT CCATTGACCC TGCAACCTTT
    TCCAGCAACT GTTTAATGCC ACAGCTAAAC GAAACAACTG CACGTAACCA AACTGAGCTT
    AATTCTTCTG TGCTTTACAA TTCCTCTGTT GATCCTTCTA ATTGGGTTGG ACTAAAGAAG
    TCAACTCCAC TCCTTGCTTA TTTGCGGAAC AATTTGCTGA TGTTGGCCAT GTTAGCTTTT
    GAAGTAACCA TTTACCGGCA TCAGGAGTAT TACAGGTTGC GAAACAATCT CACCACACCT
    GCAACAAAAA CCATCTTTCA TGACATCACA AGACTTCACT TGGATGATGG ACTCATAAAT
    AGTGCCAAGT ATTTTGTGAA CTACTTTTTC TACAAGTTTG GATTGGAAAC ATGTTTCTTA
    CTGTCGGTAA ATGTAATCGG TCAGAGGATG GACTTTTTTG CCATGGTTCA TGCACTTTGG
    CTTTTTGGCG TATTATACCG ACGCAGAAGG AAAGCGATTG CAGAAACCTG GCACAGATAT
    TGCTGTTTTC TTGCTTGCAT TATGGCATTC CAGTATTTTA TATGTATTGG CATCCCCCCT
    GCTGCATGTA AAGACTATCC TTGGAGACAA CCTGGAGCCA ATTTTAATTC TAACATAATA
    AAGTGGTTAT ACTTTCCAGA CTTTATTGTG AGGCCTAGTC CTCTGTTTCT TGTCTATGAT
    TTTTTGCTTT TGCTTTGTGC GTCCCTTCAA AGAGAAGTGT TTGAGGAAGA AAATAAGGCA
    GCAGTTCGAA TCATGGCTGG TGACAATGTA GAAATTTGCA TGAACCTTGA TGCTGCATCT
    TTTAGCCAAC ATAATCCTGT ACCTGATTTC ATATACTGCA GATCTTATTT GGACATGGGT
    AAAGTTATAA TGTTTAGTTA CCTTTTCTGG TTTGTGCTCA CCATCATCTT CATCACGGGA
    ACTACACGGA TCAGCATATT CTGCATGGGC TACTTGGTAG CTTGCTTCTA TTTTCTATTA
    TTTGGTGGAG ATTTGCTGTT AAAACCTATA AGAATCATCC TACGCTATTG GGATTGGCTG
    ATTGCTTACA ATGTTTTTGT CATTACAATG AAAAATGTAT TATCAATAGG AGCATGTGGC
    TACATTGAAT ATTTAATCAA AGACAGTTGT TGGTTAATTC AGGCATTCAG TCTTTCTTGT
    ACAGTGAAAG GTTATTTTAT CCCCCAAAAC ACACCTGACT GTCAGTTGCC AAGCGGAGAG
    GCTGGAATCA TTTGGGACAG TATATGTTTT GCTTTCCTTT TATTGCAAAG AAGAGTGTTT
    ATGAGCTATT ACTTCTTACA TGTGGTTGCA GACATCAAAT CATCTCAGAT TCTAGCATCA
    AGAGGTGCTG AACTGTTCCA AGCTACAATT GTAAAAGCTG TAAAAGCAAG AATTGAAGAA
    GAAAAAAAAT CTATGGACCA GCTCAAGAGA CAGATGGACC ACATTAAAGC CAGGCAAGAA
    AAATATAAAA AAGGTAAAGA AAAAATGCTG AGTCTTACAC AGGATGGAGA AGGACAAGAA
    GTTCAAAAAC TAGATGATGA CGATGAAGAA GGAGACAAAG CAGACAAAGA GAAAGCCAAG
    GGCAAAAAAA AGCAGTGGTG GCGACCTTGG GTTGATCATG CTTCCATGGT GAAAAGCGGT
    GATTATTATT TGTTTGAGAC GGACAGTGAA GAAGAAGATG AGGATGACAA AAAAGAAGAA
    GAGCCTAGAA GAAAATCTGC CTTCCAGAGA GCTATTGGAA AGTTTGCATC AGCCATTATA
    GCCTTGCCAA AGTCTGTCAT TAAACTGCCT AAAACAATTC TTCAATATTT AATCAGAGCT
    GCAAAGTTTT TGTACCAAGC ATGGATTACT GATCCAAAAA CAGCCCTTAA GCAAAGAAGC
    ATGGAAGCCA AAGAAGAAAA GGCAAGGGAA ATGAGGCGAA AGGGCATTGC TGAAGGTGAT
    ATTATTGTAG AATGTGGGGA TAAAGAAGAA GAAAAAGAGC AAGGAAAAAA GAAATCAGAT
    GGCCCAGACA ATATCTTCAA AAGAATATTT AACATCCTGA AGTTTACTTG GGTCCTCTTC
    CTTGCTACAA TCGATAGTTT TACGGCTTGG CTAAACTCCA TTTCAAGAGA ACATATTGAT
    ATCTCCAAAG TTCTAAGCAT AGAACGTTGC ATGCTGACTA GAGAGATAAA AAAGGGCAAT
    GTGCCTACTC GAGAAAGCAT CCACATGTAT TACCAAAATC AAATAATGAT GAGCCTTTCT
    AGAGAGTCTG GACTTGACAG AATTGATGAC CAACCTGGTT CATCGTCCAG TCACCATAGC
    CATGACAAGA TGGCGAGTAA AAGGATGGAC AGTGCAGACT CAGCTGTATC TCATGATAGC
    ATATCAAGTG AGCCAACTCA GTGCACCATG GTCTACTCAC GGCAGGGCAC AACAGAAACT
    ATTGAGGAAG TGGAAGATGA AGAGGAGGAA GAAAGCAAAA GTCCTTTATC AAAGATAGAA
    GCCGAGGAAG AAGAATATGA CATGGATTTG GAAGGGACAA GCTATACTCC GGACACAGAA
    TTTCCCCTGT ACGCCACTAC AGAGGGGGAT CTTCCTCCTT CGTACAGTAA AGCCGGCAGC
    TTCGAACAAC TTACATTTGT ATCTCAGGAC TCGGCTGACA ATTTAATGGT TCTAAGCCCA
    GATGAAAGTC GTACTGATAA GCTACATGAT CATATTCTTC CACCTCTTAC ACATGAACTG
    ACTGCTAGCG AGCTGCTTCT AAACAAAATG TTTTATGATG AAGAGTTAGA AGACTCTGAA
    AAATTTTATA CTACTCAACC ACGCCCGCTG CAGCTTATTT ATGCCTTGTA TAATACATTG
    GTGGCCAGAT CAGAAATGGT GTGCTATTTT GTGATTATTC TTAATCACAT GATTTCAGCT
    TCTATGATCA CATTGGTACT GCCCATCCTT ATATTCCTTT GGGCTATGCT TTCTGTCCCA
    AGGCCCAGTA AGAGATTTTG GATGACAGCT ATTGTATATA CAGAGGTGAC AATTGTTATT
    AAATATTTTT TCCAATTTGG ATTCTTCCCA TGGAATAAAA ATGTGGATTA TACAAAGGAC
    AAACCTTACT TCCCACCTAA CATAATGGGC ATTGAGAAGA AAGAAGGTTA TGTGCACTAT
    GACCTGGTGC AACTGCTTGC TCTGTTTTTC CATAGATCTA TTTTAAAGTG CCACGGGCTT
    TGGGATGAAG ATGATGTCAC TGAAAAAGCC ACAGATAAAG ACGACACGGA TGGAGAGATA
    TCAGAGGCAT CAGGAAGAAG GGATTCCGTT GGCTCATTGA AATCTGTCAA TCTAGCTGCG
    TCTGTGGAGT CAGTCCATGT GCACTTCCCT GAGGAAAAGA CCGCTATCAG GAGAAAAAGC
    ACAAGCAGTG GATCGCAAAT TTCCCGAAGG TCAAGTTATT CCTCGCACAG GTCTAAAAGA
    GGCAGCACCA GTACTCGGAA CAGCAGTCAA AAAGGAAGCA GCATTCCCAG TATTAAACTG
    AAATCTAGAA AGGAACTTAT ACTGGAAAAA CTCAGAGAAC AGCTCATTAA AGCAAAAGCA
    TTTACGATCA AGACGATACT ACAAATCTAT GTCCCGATCA GACAGTTTTT TTACAATCTT
    ATACATCCTG AATACAGTGC TGTGACTGAT GTCTATGTGC TGATGTTCCT GGCAGACACT
    GTAGATTTCA TCATTATTGT TTTTGGATTT TGGGCGTTTG GAAAACACTC CGCTGCAGCA
    GATATTACAT CCTCTCTATC AGAAGACCAA GTCCCAGAAG CATTTTTGGT AATGGTGCTT
    ATCCAGTTTG GAACCATGGT TGTTGATCGT GCTTTGTATC TTCGCAAAAC AGTCATGGGA
    AAAGTTATTT TCCAAGTTAT CCTTGTATTT GGAATCCATT TTTGGATGTT CTTCATCTTG
    CCAGGGGTAA CAGAAAGGAA ATTTAGTCAG AACACTGTTG CCCAACTGTG GTATTTTGTC
    AAGTGCATTT ATTTTGGATT GTCGGCATAT CAGATACGTT GTGGATATCC AACTCGGGTG
    CTTGGTAACT TCCTTACTAA AAGCTACAAT TATGTCAACC TGTTCCTTTT CCAAGGCTTT
    CGCCTCGTTC CATTTCTTAC GGAGCTGCGA GCTGTTATGG ACTGGGTATG GACTGACACC
    ACTCTCAGTC TTTCCAGCTG GATCTGTGTA GAGGACATTT ATGCACATAT ATTTATACTA
    AAATGCTGGC GGGAGTCAGA AAAGAGATAT CCCCAGCCAC GAGGCCAAAA GAAGAAAAAG
    GTTGTGAAAT ACGGAATGGG TGGAATGATA ATAGTTCTGC TGATTTGTAT CGTGTGGTTT
    CCTTTGCTCT TCATGTCCTT GATTAAATCA GTTGCTGGGG TCACTAACAA GCCCCTAGAT
    GTGTCCATTA CAATAACCCT TGGAGGTTAT CAGCCTATTT TTACCATGAG TGCACAACAA
    AATCAGCTGC AAGGCTTGAA TGACAATGAA TTTAAGCATT TACATAATAT ATATAAAGGC
    AATACACCTG CCATGCAGTT TCTGGAATCC TATATGCAGG AAGACATCAC CATAGCAGGA
    CTGGAAGGCA ATTCCAACTC TCTGTGGACC ATCAGCCCTC CGAGCAGGAC AATGATGATA
    GAAAGACTTA TCCAGGAACC TGACTTCACA GCGGTCATTT CTTGGTCCAT TCGAAGAAAT
    GTGAGTCTTG GAGCTAAGGC AGAGGTAACA GGTGAAAAAT ATACAATATC ACTGGCAGGT
    CAAACTCGGA GGGAACTTGC GGATATGATG AATAGTTCAT CTCCCAATAA GACAGTGGAA
    CTGATAAAGA TATACCCATT GTGTTTAAAA GCACCAAGTG ATGTTACTGC AAAACCCATC
    CTTCAGCTTC TTAAGAATCA AGAGTTTGTG AATATTAGCG TTTTACTAAG AAAAGAAAAC
    ACATCAGAAG GGGTTCGAGA ATGGTGGGTA GTGAATCAAA GCCATAAAGA GTTCATTAAG
    AAGGATGGCC TGGAACTATG GGTTTTCAGT GACAAAGTCA GTCCTCCAAG TCTTGGATTC
    TTGGCAGGCT ATGGAATAAT GGGACTATAT GCCTCTGTAG TTCTCGTAAT AGGAAAGTTT
    GTGCGTGAGT TTTTCAGCGG AATTTCGCAC TCCATCATGT TTGAGGAGCT TCCCAACGTT
    GACCGTATAC TGAAGCTGTG CACAGACATT TTCCTTGTGA GAGAGACTGG AGAGTTGGAA
    CTGGAGGAAG ACCTGTATGC CAAATTAATA TTTTTGTACC GCTCCCCAGA AACTATGATC
    AAATGGACAA GAGAGAAAGA TAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa60022
    Clone ID Related Accession (Same CDS sequence) XM_012965363.3
    Accession Version XM_012965363.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8208bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product piezo-type mechanosensitive ion channel component 2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012965363.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    ATGGCATCGG AAGTGGTGTG TGGGCTCATC TTCAGATTTC TTCTCCCACT ATGCCTGGTT 
    GTAGCGTGCA TGTTCCGGTA CAATGGAATA TCTTTTGTCT ACCTCATCTA CCTCTTGCTT
    ATACCTTTAT TTTCGGAGCC TACCAAAATA ACAATGCAAG GTCATACAGG ACGGTTATTA
    AAATCCTTAT GTGTTTCCAG TCTGTCATTT CTGCTGCTGC ACATCATCTT TCAAATTACC
    GTATACAGCC TCGAGGCCAG AAAACACATT GAACCACATT TTAACTGTTC AACATGGGAG
    AAAGCAATAC GACAAATTGG TTTTGAAAGT GTAAAAGGAG CAGATGCTGG TAATGGAATC
    CGCGTGTTTG CACCAGATAT CGGAATGTTT ATTGCAAGCT TGGCTACCCT GTTGGTTTGT
    AGAAAACTAG TTCAGAAAAC AGTTACTGAA GAGGACGCAC AATACAACCC CCAGTTTGTA
    AATGAAGAAA TGGCAGAAGA AGGAAAGGTG GACCTGGATG AAGCGTTAAT GTATGAGGAA
    GACTTTGATG GAGAAGAGGA TGAAATTGAG GCAGCTGAAG AAAGCACAAA ATTAAAAATT
    CTTCGCAGAA TTGCTTCATT TGCATCCGTA CTTAAAGAGT TTATTGGCAA CATTATAACC
    ACAGCTGGGA AAGTAGTTAT TACAATTTTA CTTGGTTTAA CAGGCATGAT GTTGCCGTCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT TTGGGGCTAT GCACATGGTG GTCCTGTTGC
    CGAGTATTTG ATCCGCTGAT ATTCAGCTGT CTATGTGTAT TAATGGCTAT ATTTAGTGCA
    GGGCACTTAA TTGGACTTTA TCTGTACCAG TTGCAGTTTT TTCACGAGAT TATTCCACCA
    AATGATTTCT ATGCCAGGTT GTTTGGTGTT ACTTCATTAG TTCAAACCGA CTGCTGTAAC
    ACTTGGAGAT TTTTTGCAAA CAACAGTCTA CCATGGAACC AGTATACCAA TCCTGTCATG
    CTTTTAGTAC TGTATTACAC GCTGGCAACT CTCATACGCC TTTGGCTACA GGAACCTATT
    AATGTAGAGG AAGAGAAGGA GGAAGACTGT AGCCCAGCAG CACTTACTGC AGAAAGAAGA
    CGTAGTTTGT GGTACTCTAG GCACTACACA ACGGATGAAA GACAACTTCT GTCCATGACT
    CAAGATGACT ACAAGCCATC TGATGTTTTA CTAGTAACTG TAAATGGGAC TCCCGTAGAT
    TACTGTTCAC TAAATTCATC ACTTCCGATA GAAAATGGTC CAACTAAAAT TGACATGTAT
    TCAACACCGC AATATAAATG GGACCATGAA GATGAAACTT CAGAAAAGAA AGAAGAGGAA
    GAGCAAGAAG AAGAACCCAA TGAAGAGGTT GCAGAAGAGA AAGAAAACAC TAAAGTTCAT
    GTAATGGTGG TGGCATTTCA GTTCATTATG AAGCAAAGCT ATATATGTGC ACTAATTTCA
    ATGATGGCCT GGAGCATCAC ATACCACAGC TGGTTGACTT TTGTTTTGTT AATCTGGTCT
    TGTGCTCTCT GGATGGTCCG AAATAGGAGA AAATATGCTA TGCTTAGTTC ACCTTTTATG
    GTTATGTATG GTAATTTACT TTTAACATTA CAATACATAT GGAGCATAGC CTTACAAAGT
    GATGAACTTC CAGAAGTTAT GGGTCTCCTT GAGAGAAAAA AGCCCGAAGA GCTTGTATCG
    AAGATCATTT TCACAGTTAC ATTTTGGCTT TTGCTCAGAC AACACCTTAC AGAGCAAAAG
    GCATTAAAGC AAAAAGAATT AGCATTATCT GAAATAAAAG TTGGAAGTGA AGACAAAGAA
    GGTGAAGTGC AAGAAGAAGA AGAAGAAGTG GAGGATGAAA ATGATATCAT GAAGGTCCTC
    GGAAAGCTAG TGCAGGCTAT GTTTGTCAAG TACTGGATTT ATGTCTGTGG CGGCATGTTT
    TTTTTTGTCA GCTTTGAAGG AAAAATAGTT ATGTACAAAA TTATCTACAT GATGCTCTTC
    CTATTCTGTG TAGCTCTGTA TCAGGTGCAC TATGAATGGT GGAGACGAAT TTTAAAATAT
    TTTTGGATGT CTGTGGTTGT TTACACCATG TTGGTGCTTG TTTTTATTTA CACTTACCAA
    TTTAAATCAT TTCCGGGCCT TTGGATGAAT ATGACAGGGC TGGATAAGGA AAAGTTAGCA
    GATCTTGGTT TAGAGACATT TAACTTGACT GACCTATTTA CTCGTATATT TATCCCAACC
    TCATTTTTGT TAGTGTGCAT TTTGCACCTA CATTACTTCC ATGACCGTTT TCTCCAGCTC
    ACTGACCTAA AGGCTGTAAC CAGCAAGCAG GACAGCACAA TTTACAGCCA TGCCAAAGTA
    AATGGTCGAG TTTACTTAAT AATAAATAGC ATCAAGCAAA AAATTCCAAT TCAGCAAAAT
    GAATTGGTGC ATCCAGAAGG AAGTTTGCCT GATATAACGA TGATGAACTT AACAACAGAA
    TCTGAAATGC TTGAGGAAAA GCGGTTGGAA ATTTATGACG AAAAAAAAAC TCATGACCAA
    GACTCAGATG ATGAATGGGA TAAAGGGGAT AGCAAAGAAC CAGGTCATTC TGATGATGAA
    GCTGAGGATG AGGAATCAGA AGAAGAAGAA ACAACAACAG AGTCCAGGAA CAAGTGGCAT
    CTAGTGATAG ATCGCTTAAC GGTTCTTTTC CTAAAGTTTC TGGAATATTT TCACAAGTTT
    CAGGTCTTTG TTTGGTGGCT TCTGGAGCTT CACATGATAA AGATTGTTTC TTCATACATC
    ATTTGGCTTA CTATAAAGGA GGTTTCACTG CTCAATTATG TATTTTTTAT TGCCTGGGCC
    TTCGCTTTGC CCTACTCACA ATTCCGGCCA TTGGCATCAA GTGTTTGCAC AGTCTGGACA
    TGTGTCATCA TCATATGTAA AATGTTGTAC CAGCTAGCCT CCATTGACCC TGCAACCTTT
    TCCAGCAACT GTTTAATGCC ACAGCTAAAC GAAACAACTG CACGTAACCA AACTGAGCTT
    AATTCTTCTG TGCTTTACAA TTCCTCTGTT GATCCTTCTA ATTGGGTTGG ACTAAAGAAG
    TCAACTCCAC TCCTTGCTTA TTTGCGGAAC AATTTGCTGA TGTTGGCCAT GTTAGCTTTT
    GAAGTAACCA TTTACCGGCA TCAGGAGTAT TACAGGTTGC GAAACAATCT CACCACACCT
    GCAACAAAAA CCATCTTTCA TGACATCACA AGACTTCACT TGGATGATGG ACTCATAAAT
    AGTGCCAAGT ATTTTGTGAA CTACTTTTTC TACAAGTTTG GATTGGAAAC ATGTTTCTTA
    CTGTCGGTAA ATGTAATCGG TCAGAGGATG GACTTTTTTG CCATGGTTCA TGCACTTTGG
    CTTTTTGGCG TATTATACCG ACGCAGAAGG AAAGCGATTG CAGAAACCTG GCACAGATAT
    TGCTGTTTTC TTGCTTGCAT TATGGCATTC CAGTATTTTA TATGTATTGG CATCCCCCCT
    GCTGCATGTA AAGACTATCC TTGGAGACAA CCTGGAGCCA ATTTTAATTC TAACATAATA
    AAGTGGTTAT ACTTTCCAGA CTTTATTGTG AGGCCTAGTC CTCTGTTTCT TGTCTATGAT
    TTTTTGCTTT TGCTTTGTGC GTCCCTTCAA AGAGAAGTGT TTGAGGAAGA AAATAAGGCA
    GCAGTTCGAA TCATGGCTGG TGACAATGTA GAAATTTGCA TGAACCTTGA TGCTGCATCT
    TTTAGCCAAC ATAATCCTGT ACCTGATTTC ATATACTGCA GATCTTATTT GGACATGGGT
    AAAGTTATAA TGTTTAGTTA CCTTTTCTGG TTTGTGCTCA CCATCATCTT CATCACGGGA
    ACTACACGGA TCAGCATATT CTGCATGGGC TACTTGGTAG CTTGCTTCTA TTTTCTATTA
    TTTGGTGGAG ATTTGCTGTT AAAACCTATA AGAATCATCC TACGCTATTG GGATTGGCTG
    ATTGCTTACA ATGTTTTTGT CATTACAATG AAAAATGTAT TATCAATAGG AGCATGTGGC
    TACATTGAAT ATTTAATCAA AGACAGTTGT TGGTTAATTC AGGCATTCAG TCTTTCTTGT
    ACAGTGAAAG GTTATTTTAT CCCCCAAAAC ACACCTGACT GTCAGTTGCC AAGCGGAGAG
    GCTGGAATCA TTTGGGACAG TATATGTTTT GCTTTCCTTT TATTGCAAAG AAGAGTGTTT
    ATGAGCTATT ACTTCTTACA TGTGGTTGCA GACATCAAAT CATCTCAGAT TCTAGCATCA
    AGAGGTGCTG AACTGTTCCA AGCTACAATT GTAAAAGCTG TAAAAGCAAG AATTGAAGAA
    GAAAAAAAAT CTATGGACCA GCTCAAGAGA CAGATGGACC ACATTAAAGC CAGGCAAGAA
    AAATATAAAA AAGGTAAAGA AAAAATGCTG AGTCTTACAC AGGATGGAGA AGGACAAGAA
    GTTCAAAAAC TAGATGATGA CGATGAAGAA GGAGACAAAG CAGACAAAGA GAAAGCCAAG
    GGCAAAAAAA AGCAGTGGTG GCGACCTTGG GTTGATCATG CTTCCATGGT GAAAAGCGGT
    GATTATTATT TGTTTGAGAC GGACAGTGAA GAAGAAGATG AGGATGACAA AAAAGAAGAA
    GAGCCTAGAA GAAAATCTGC CTTCCAGTTT TTGTACCAAG CATGGATTAC TGATCCAAAA
    ACAGCCCTTA AGCAAAGAAG CATGGAAGCC AAAGAAGAAA AGGCAAGGGA AATGAGGCGA
    AAGGGCATTG CTGAAGGTGA TATTATTGTA GAATGTGGGG ATAAAGAAGA AGAAAAAGAG
    CAAGGAAAAA AGAAATCAGA TGGCCCAGAC AATATCTTCA AAAGAATATT TAACATCCTG
    AAGTTTACTT GGGTCCTCTT CCTTGCTACA ATCGATAGTT TTACGGCTTG GCTAAACTCC
    ATTTCAAGAG AACATATTGA TATCTCCAAA GTTCTAAGCA TAGAACGTTG CATGCTGACT
    AGAGAGATAA AAAAGGGCAA TGTGCCTACT CGAGAAAGCA TCCACATGTA TTACCAAAAT
    CAAATAATGA TGAGCCTTTC TAGAGAGTCT GGACTTGACA GAATTGATGA CCAACCTGGT
    TCATCGTCCA GTCACCATAG CCATGACAAG ATGGCGAGTA AAAGGATGGA CAGTGCAGAC
    TCAGCTGTAT CTCATGATAG CATATCAAGT GAGCCAACTC AGTGCACCAT GGTCTACTCA
    CGGCAGGGCA CAACAGAAAC TATTGAGGAA GTGGAAGATG AAGAGGAGGA AGAAAGCAAA
    AGTCCTTTAT CAAAGATAGA AGCCGAGGAA GAAGAATATG ACATGGATTT GGAAGGGACA
    AGCTATACTC CGGACACAGA ATTTCCCCTG TACGCCACTA CAGAGGGGGA TCTTCCTCCT
    TCGTACAGTA AAGCCGGCAG CTTCGAACAA CTTACATTTG TATCTCAGGA CTCGGCTGAC
    AATTTAATGG TTCTAAGCCC AGATGAAAGT CGTACTGATA AGCTACATGA TCATATTCTT
    CCACCTCTTA CACATGAACT GACTGCTAGC GAGCTGCTTC TAAACAAAAT GTTTTATGAT
    GAAGAGTTAG AAGACTCTGA AAAATTTTAT ACTACTCAAC CACGCCCGCT GCAGCTTATT
    TATGCCTTGT ATAATACATT GGTGGCCAGA TCAGAAATGG TGTGCTATTT TGTGATTATT
    CTTAATCACA TGATTTCAGC TTCTATGATC ACATTGGTAC TGCCCATCCT TATATTCCTT
    TGGGCTATGC TTTCTGTCCC AAGGCCCAGT AAGAGATTTT GGATGACAGC TATTGTATAT
    ACAGAGGTGA CAATTGTTAT TAAATATTTT TTCCAATTTG GATTCTTCCC ATGGAATAAA
    AATGTGGATT ATACAAAGGA CAAACCTTAC TTCCCACCTA ACATAATGGG CATTGAGAAG
    AAAGAAGGTT ATGTGCACTA TGACCTGGTG CAACTGCTTG CTCTGTTTTT CCATAGATCT
    ATTTTAAAGT GCCACGGGCT TTGGGATGAA GATGATGTCA CTGAAAAAGC CACAGATAAA
    GACGACACGG ATGGAGAGAT ATCAGAGGCA TCAGGAAGAA GGGATTCCGT TGGCTCATTG
    AAATCTGTCA ATCTAGCTGC GTCTGTGGAG TCAGTCCATG TGCACTTCCC TGAGGAAAAG
    ACCGCTATCA GGAGAAAAAG CACAAGCAGT GGATCGCAAA TTTCCCGAAG GTCAAGTTAT
    TCCTCGCACA GGTCTAAAAG AGGCAGCACC AGTACTCGGA ACAGCAGTCA AAAAGGAAGC
    AGCATTCCCA GTATTAAACT GAAATCTAGA AAGGAACTTA TACTGGAAAA ACTCAGAGAA
    CAGCTCATTA AAGCAAAAGC ATTTACGATC AAGACGATAC TACAAATCTA TGTCCCGATC
    AGACAGTTTT TTTACAATCT TATACATCCT GAATACAGTG CTGTGACTGA TGTCTATGTG
    CTGATGTTCC TGGCAGACAC TGTAGATTTC ATCATTATTG TTTTTGGATT TTGGGCGTTT
    GGAAAACACT CCGCTGCAGC AGATATTACA TCCTCTCTAT CAGAAGACCA AGTCCCAGAA
    GCATTTTTGG TAATGGTGCT TATCCAGTTT GGAACCATGG TTGTTGATCG TGCTTTGTAT
    CTTCGCAAAA CAGTCATGGG AAAAGTTATT TTCCAAGTTA TCCTTGTATT TGGAATCCAT
    TTTTGGATGT TCTTCATCTT GCCAGGGGTA ACAGAAAGGA AATTTAGTCA GAACACTGTT
    GCCCAACTGT GGTATTTTGT CAAGTGCATT TATTTTGGAT TGTCGGCATA TCAGATACGT
    TGTGGATATC CAACTCGGGT GCTTGGTAAC TTCCTTACTA AAAGCTACAA TTATGTCAAC
    CTGTTCCTTT TCCAAGGCTT TCGCCTCGTT CCATTTCTTA CGGAGCTGCG AGCTGTTATG
    GACTGGGTAT GGACTGACAC CACTCTCAGT CTTTCCAGCT GGATCTGTGT AGAGGACATT
    TATGCACATA TATTTATACT AAAATGCTGG CGGGAGTCAG AAAAGAGATA TCCCCAGCCA
    CGAGGCCAAA AGAAGAAAAA GGTTGTGAAA TACGGAATGG GTGGAATGAT AATAGTTCTG
    CTGATTTGTA TCGTGTGGTT TCCTTTGCTC TTCATGTCCT TGATTAAATC AGTTGCTGGG
    GTCACTAACA AGCCCCTAGA TGTGTCCATT ACAATAACCC TTGGAGGTTA TCAGCCTATT
    TTTACCATGA GTGCACAACA AAATCAGCTG CAAGGCTTGA ATGACAATGA ATTTAAGCAT
    TTACATAATA TATATAAAGG CAATACACCT GCCATGCAGT TTCTGGAATC CTATATGCAG
    GAAGACATCA CCATAGCAGG ACTGGAAGGC AATTCCAACT CTCTGTGGAC CATCAGCCCT
    CCGAGCAGGA CAATGATGAT AGAAAGACTT ATCCAGGAAC CTGACTTCAC AGCGGTCATT
    TCTTGGTCCA TTCGAAGAAA TGTGAGTCTT GGAGCTAAGG CAGAGGTAAC AGGTGAAAAA
    TATACAATAT CACTGGCAGG TCAAACTCGG AGGGAACTTG CGGATATGAT GAATAGTTCA
    TCTCCCAATA AGACAGTGGA ACTGATAAAG ATATACCCAT TGTGTTTAAA AGCACCAAGT
    GATGTTACTG CAAAACCCAT CCTTCAGCTT CTTAAGAATC AAGAGTTTGT GAATATTAGC
    GTTTTACTAA GAAAAGAAAA CACATCAGAA GGGGTTCGAG AATGGTGGGT AGTGAATCAA
    AGCCATAAAG AGTTCATTAA GAAGGATGGC CTGGAACTAT GGGTTTTCAG TGACAAAGTC
    AGTCCTCCAA GTCTTGGATT CTTGGCAGGC TATGGAATAA TGGGACTATA TGCCTCTGTA
    GTTCTCGTAA TAGGAAAGTT TGTGCGTGAG TTTTTCAGCG GAATTTCGCA CTCCATCATG
    TTTGAGGAGC TTCCCAACGT TGACCGTATA CTGAAGCTGT GCACAGACAT TTTCCTTGTG
    AGAGAGACTG GAGAGTTGGA ACTGGAGGAA GACCTGTATG CCAAATTAAT ATTTTTGTAC
    CGCTCCCCAG AAACTATGAT CAAATGGACA AGAGAGAAAG ATAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012820817.2
    CDS632..8839
    Translation

    Target ORF information:

    RefSeq Version XM_012965363.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis piezo-type mechanosensitive ion channel component 2 (piezo2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012965363.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    ATGGCATCGG AAGTGGTGTG TGGGCTCATC TTCAGATTTC TTCTCCCACT ATGCCTGGTT 
    GTAGCGTGCA TGTTCCGGTA CAATGGAATA TCTTTTGTCT ACCTCATCTA CCTCTTGCTT
    ATACCTTTAT TTTCGGAGCC TACCAAAATA ACAATGCAAG GTCATACAGG ACGGTTATTA
    AAATCCTTAT GTGTTTCCAG TCTGTCATTT CTGCTGCTGC ACATCATCTT TCAAATTACC
    GTATACAGCC TCGAGGCCAG AAAACACATT GAACCACATT TTAACTGTTC AACATGGGAG
    AAAGCAATAC GACAAATTGG TTTTGAAAGT GTAAAAGGAG CAGATGCTGG TAATGGAATC
    CGCGTGTTTG CACCAGATAT CGGAATGTTT ATTGCAAGCT TGGCTACCCT GTTGGTTTGT
    AGAAAACTAG TTCAGAAAAC AGTTACTGAA GAGGACGCAC AATACAACCC CCAGTTTGTA
    AATGAAGAAA TGGCAGAAGA AGGAAAGGTG GACCTGGATG AAGCGTTAAT GTATGAGGAA
    GACTTTGATG GAGAAGAGGA TGAAATTGAG GCAGCTGAAG AAAGCACAAA ATTAAAAATT
    CTTCGCAGAA TTGCTTCATT TGCATCCGTA CTTAAAGAGT TTATTGGCAA CATTATAACC
    ACAGCTGGGA AAGTAGTTAT TACAATTTTA CTTGGTTTAA CAGGCATGAT GTTGCCGTCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT TTGGGGCTAT GCACATGGTG GTCCTGTTGC
    CGAGTATTTG ATCCGCTGAT ATTCAGCTGT CTATGTGTAT TAATGGCTAT ATTTAGTGCA
    GGGCACTTAA TTGGACTTTA TCTGTACCAG TTGCAGTTTT TTCACGAGAT TATTCCACCA
    AATGATTTCT ATGCCAGGTT GTTTGGTGTT ACTTCATTAG TTCAAACCGA CTGCTGTAAC
    ACTTGGAGAT TTTTTGCAAA CAACAGTCTA CCATGGAACC AGTATACCAA TCCTGTCATG
    CTTTTAGTAC TGTATTACAC GCTGGCAACT CTCATACGCC TTTGGCTACA GGAACCTATT
    AATGTAGAGG AAGAGAAGGA GGAAGACTGT AGCCCAGCAG CACTTACTGC AGAAAGAAGA
    CGTAGTTTGT GGTACTCTAG GCACTACACA ACGGATGAAA GACAACTTCT GTCCATGACT
    CAAGATGACT ACAAGCCATC TGATGTTTTA CTAGTAACTG TAAATGGGAC TCCCGTAGAT
    TACTGTTCAC TAAATTCATC ACTTCCGATA GAAAATGGTC CAACTAAAAT TGACATGTAT
    TCAACACCGC AATATAAATG GGACCATGAA GATGAAACTT CAGAAAAGAA AGAAGAGGAA
    GAGCAAGAAG AAGAACCCAA TGAAGAGGTT GCAGAAGAGA AAGAAAACAC TAAAGTTCAT
    GTAATGGTGG TGGCATTTCA GTTCATTATG AAGCAAAGCT ATATATGTGC ACTAATTTCA
    ATGATGGCCT GGAGCATCAC ATACCACAGC TGGTTGACTT TTGTTTTGTT AATCTGGTCT
    TGTGCTCTCT GGATGGTCCG AAATAGGAGA AAATATGCTA TGCTTAGTTC ACCTTTTATG
    GTTATGTATG GTAATTTACT TTTAACATTA CAATACATAT GGAGCATAGC CTTACAAAGT
    GATGAACTTC CAGAAGTTAT GGGTCTCCTT GAGAGAAAAA AGCCCGAAGA GCTTGTATCG
    AAGATCATTT TCACAGTTAC ATTTTGGCTT TTGCTCAGAC AACACCTTAC AGAGCAAAAG
    GCATTAAAGC AAAAAGAATT AGCATTATCT GAAATAAAAG TTGGAAGTGA AGACAAAGAA
    GGTGAAGTGC AAGAAGAAGA AGAAGAAGTG GAGGATGAAA ATGATATCAT GAAGGTCCTC
    GGAAAGCTAG TGCAGGCTAT GTTTGTCAAG TACTGGATTT ATGTCTGTGG CGGCATGTTT
    TTTTTTGTCA GCTTTGAAGG AAAAATAGTT ATGTACAAAA TTATCTACAT GATGCTCTTC
    CTATTCTGTG TAGCTCTGTA TCAGGTGCAC TATGAATGGT GGAGACGAAT TTTAAAATAT
    TTTTGGATGT CTGTGGTTGT TTACACCATG TTGGTGCTTG TTTTTATTTA CACTTACCAA
    TTTAAATCAT TTCCGGGCCT TTGGATGAAT ATGACAGGGC TGGATAAGGA AAAGTTAGCA
    GATCTTGGTT TAGAGACATT TAACTTGACT GACCTATTTA CTCGTATATT TATCCCAACC
    TCATTTTTGT TAGTGTGCAT TTTGCACCTA CATTACTTCC ATGACCGTTT TCTCCAGCTC
    ACTGACCTAA AGGCTGTAAC CAGCAAGCAG GACAGCACAA TTTACAGCCA TGCCAAAGTA
    AATGGTCGAG TTTACTTAAT AATAAATAGC ATCAAGCAAA AAATTCCAAT TCAGCAAAAT
    GAATTGGTGC ATCCAGAAGG AAGTTTGCCT GATATAACGA TGATGAACTT AACAACAGAA
    TCTGAAATGC TTGAGGAAAA GCGGTTGGAA ATTTATGACG AAAAAAAAAC TCATGACCAA
    GACTCAGATG ATGAATGGGA TAAAGGGGAT AGCAAAGAAC CAGGTCATTC TGATGATGAA
    GCTGAGGATG AGGAATCAGA AGAAGAAGAA ACAACAACAG AGTCCAGGAA CAAGTGGCAT
    CTAGTGATAG ATCGCTTAAC GGTTCTTTTC CTAAAGTTTC TGGAATATTT TCACAAGTTT
    CAGGTCTTTG TTTGGTGGCT TCTGGAGCTT CACATGATAA AGATTGTTTC TTCATACATC
    ATTTGGCTTA CTATAAAGGA GGTTTCACTG CTCAATTATG TATTTTTTAT TGCCTGGGCC
    TTCGCTTTGC CCTACTCACA ATTCCGGCCA TTGGCATCAA GTGTTTGCAC AGTCTGGACA
    TGTGTCATCA TCATATGTAA AATGTTGTAC CAGCTAGCCT CCATTGACCC TGCAACCTTT
    TCCAGCAACT GTTTAATGCC ACAGCTAAAC GAAACAACTG CACGTAACCA AACTGAGCTT
    AATTCTTCTG TGCTTTACAA TTCCTCTGTT GATCCTTCTA ATTGGGTTGG ACTAAAGAAG
    TCAACTCCAC TCCTTGCTTA TTTGCGGAAC AATTTGCTGA TGTTGGCCAT GTTAGCTTTT
    GAAGTAACCA TTTACCGGCA TCAGGAGTAT TACAGGTTGC GAAACAATCT CACCACACCT
    GCAACAAAAA CCATCTTTCA TGACATCACA AGACTTCACT TGGATGATGG ACTCATAAAT
    AGTGCCAAGT ATTTTGTGAA CTACTTTTTC TACAAGTTTG GATTGGAAAC ATGTTTCTTA
    CTGTCGGTAA ATGTAATCGG TCAGAGGATG GACTTTTTTG CCATGGTTCA TGCACTTTGG
    CTTTTTGGCG TATTATACCG ACGCAGAAGG AAAGCGATTG CAGAAACCTG GCACAGATAT
    TGCTGTTTTC TTGCTTGCAT TATGGCATTC CAGTATTTTA TATGTATTGG CATCCCCCCT
    GCTGCATGTA AAGACTATCC TTGGAGACAA CCTGGAGCCA ATTTTAATTC TAACATAATA
    AAGTGGTTAT ACTTTCCAGA CTTTATTGTG AGGCCTAGTC CTCTGTTTCT TGTCTATGAT
    TTTTTGCTTT TGCTTTGTGC GTCCCTTCAA AGAGAAGTGT TTGAGGAAGA AAATAAGGCA
    GCAGTTCGAA TCATGGCTGG TGACAATGTA GAAATTTGCA TGAACCTTGA TGCTGCATCT
    TTTAGCCAAC ATAATCCTGT ACCTGATTTC ATATACTGCA GATCTTATTT GGACATGGGT
    AAAGTTATAA TGTTTAGTTA CCTTTTCTGG TTTGTGCTCA CCATCATCTT CATCACGGGA
    ACTACACGGA TCAGCATATT CTGCATGGGC TACTTGGTAG CTTGCTTCTA TTTTCTATTA
    TTTGGTGGAG ATTTGCTGTT AAAACCTATA AGAATCATCC TACGCTATTG GGATTGGCTG
    ATTGCTTACA ATGTTTTTGT CATTACAATG AAAAATGTAT TATCAATAGG AGCATGTGGC
    TACATTGAAT ATTTAATCAA AGACAGTTGT TGGTTAATTC AGGCATTCAG TCTTTCTTGT
    ACAGTGAAAG GTTATTTTAT CCCCCAAAAC ACACCTGACT GTCAGTTGCC AAGCGGAGAG
    GCTGGAATCA TTTGGGACAG TATATGTTTT GCTTTCCTTT TATTGCAAAG AAGAGTGTTT
    ATGAGCTATT ACTTCTTACA TGTGGTTGCA GACATCAAAT CATCTCAGAT TCTAGCATCA
    AGAGGTGCTG AACTGTTCCA AGCTACAATT GTAAAAGCTG TAAAAGCAAG AATTGAAGAA
    GAAAAAAAAT CTATGGACCA GCTCAAGAGA CAGATGGACC ACATTAAAGC CAGGCAAGAA
    AAATATAAAA AAGGTAAAGA AAAAATGCTG AGTCTTACAC AGGATGGAGA AGGACAAGAA
    GTTCAAAAAC TAGATGATGA CGATGAAGAA GGAGACAAAG CAGACAAAGA GAAAGCCAAG
    GGCAAAAAAA AGCAGTGGTG GCGACCTTGG GTTGATCATG CTTCCATGGT GAAAAGCGGT
    GATTATTATT TGTTTGAGAC GGACAGTGAA GAAGAAGATG AGGATGACAA AAAAGAAGAA
    GAGCCTAGAA GAAAATCTGC CTTCCAGTTT TTGTACCAAG CATGGATTAC TGATCCAAAA
    ACAGCCCTTA AGCAAAGAAG CATGGAAGCC AAAGAAGAAA AGGCAAGGGA AATGAGGCGA
    AAGGGCATTG CTGAAGGTGA TATTATTGTA GAATGTGGGG ATAAAGAAGA AGAAAAAGAG
    CAAGGAAAAA AGAAATCAGA TGGCCCAGAC AATATCTTCA AAAGAATATT TAACATCCTG
    AAGTTTACTT GGGTCCTCTT CCTTGCTACA ATCGATAGTT TTACGGCTTG GCTAAACTCC
    ATTTCAAGAG AACATATTGA TATCTCCAAA GTTCTAAGCA TAGAACGTTG CATGCTGACT
    AGAGAGATAA AAAAGGGCAA TGTGCCTACT CGAGAAAGCA TCCACATGTA TTACCAAAAT
    CAAATAATGA TGAGCCTTTC TAGAGAGTCT GGACTTGACA GAATTGATGA CCAACCTGGT
    TCATCGTCCA GTCACCATAG CCATGACAAG ATGGCGAGTA AAAGGATGGA CAGTGCAGAC
    TCAGCTGTAT CTCATGATAG CATATCAAGT GAGCCAACTC AGTGCACCAT GGTCTACTCA
    CGGCAGGGCA CAACAGAAAC TATTGAGGAA GTGGAAGATG AAGAGGAGGA AGAAAGCAAA
    AGTCCTTTAT CAAAGATAGA AGCCGAGGAA GAAGAATATG ACATGGATTT GGAAGGGACA
    AGCTATACTC CGGACACAGA ATTTCCCCTG TACGCCACTA CAGAGGGGGA TCTTCCTCCT
    TCGTACAGTA AAGCCGGCAG CTTCGAACAA CTTACATTTG TATCTCAGGA CTCGGCTGAC
    AATTTAATGG TTCTAAGCCC AGATGAAAGT CGTACTGATA AGCTACATGA TCATATTCTT
    CCACCTCTTA CACATGAACT GACTGCTAGC GAGCTGCTTC TAAACAAAAT GTTTTATGAT
    GAAGAGTTAG AAGACTCTGA AAAATTTTAT ACTACTCAAC CACGCCCGCT GCAGCTTATT
    TATGCCTTGT ATAATACATT GGTGGCCAGA TCAGAAATGG TGTGCTATTT TGTGATTATT
    CTTAATCACA TGATTTCAGC TTCTATGATC ACATTGGTAC TGCCCATCCT TATATTCCTT
    TGGGCTATGC TTTCTGTCCC AAGGCCCAGT AAGAGATTTT GGATGACAGC TATTGTATAT
    ACAGAGGTGA CAATTGTTAT TAAATATTTT TTCCAATTTG GATTCTTCCC ATGGAATAAA
    AATGTGGATT ATACAAAGGA CAAACCTTAC TTCCCACCTA ACATAATGGG CATTGAGAAG
    AAAGAAGGTT ATGTGCACTA TGACCTGGTG CAACTGCTTG CTCTGTTTTT CCATAGATCT
    ATTTTAAAGT GCCACGGGCT TTGGGATGAA GATGATGTCA CTGAAAAAGC CACAGATAAA
    GACGACACGG ATGGAGAGAT ATCAGAGGCA TCAGGAAGAA GGGATTCCGT TGGCTCATTG
    AAATCTGTCA ATCTAGCTGC GTCTGTGGAG TCAGTCCATG TGCACTTCCC TGAGGAAAAG
    ACCGCTATCA GGAGAAAAAG CACAAGCAGT GGATCGCAAA TTTCCCGAAG GTCAAGTTAT
    TCCTCGCACA GGTCTAAAAG AGGCAGCACC AGTACTCGGA ACAGCAGTCA AAAAGGAAGC
    AGCATTCCCA GTATTAAACT GAAATCTAGA AAGGAACTTA TACTGGAAAA ACTCAGAGAA
    CAGCTCATTA AAGCAAAAGC ATTTACGATC AAGACGATAC TACAAATCTA TGTCCCGATC
    AGACAGTTTT TTTACAATCT TATACATCCT GAATACAGTG CTGTGACTGA TGTCTATGTG
    CTGATGTTCC TGGCAGACAC TGTAGATTTC ATCATTATTG TTTTTGGATT TTGGGCGTTT
    GGAAAACACT CCGCTGCAGC AGATATTACA TCCTCTCTAT CAGAAGACCA AGTCCCAGAA
    GCATTTTTGG TAATGGTGCT TATCCAGTTT GGAACCATGG TTGTTGATCG TGCTTTGTAT
    CTTCGCAAAA CAGTCATGGG AAAAGTTATT TTCCAAGTTA TCCTTGTATT TGGAATCCAT
    TTTTGGATGT TCTTCATCTT GCCAGGGGTA ACAGAAAGGA AATTTAGTCA GAACACTGTT
    GCCCAACTGT GGTATTTTGT CAAGTGCATT TATTTTGGAT TGTCGGCATA TCAGATACGT
    TGTGGATATC CAACTCGGGT GCTTGGTAAC TTCCTTACTA AAAGCTACAA TTATGTCAAC
    CTGTTCCTTT TCCAAGGCTT TCGCCTCGTT CCATTTCTTA CGGAGCTGCG AGCTGTTATG
    GACTGGGTAT GGACTGACAC CACTCTCAGT CTTTCCAGCT GGATCTGTGT AGAGGACATT
    TATGCACATA TATTTATACT AAAATGCTGG CGGGAGTCAG AAAAGAGATA TCCCCAGCCA
    CGAGGCCAAA AGAAGAAAAA GGTTGTGAAA TACGGAATGG GTGGAATGAT AATAGTTCTG
    CTGATTTGTA TCGTGTGGTT TCCTTTGCTC TTCATGTCCT TGATTAAATC AGTTGCTGGG
    GTCACTAACA AGCCCCTAGA TGTGTCCATT ACAATAACCC TTGGAGGTTA TCAGCCTATT
    TTTACCATGA GTGCACAACA AAATCAGCTG CAAGGCTTGA ATGACAATGA ATTTAAGCAT
    TTACATAATA TATATAAAGG CAATACACCT GCCATGCAGT TTCTGGAATC CTATATGCAG
    GAAGACATCA CCATAGCAGG ACTGGAAGGC AATTCCAACT CTCTGTGGAC CATCAGCCCT
    CCGAGCAGGA CAATGATGAT AGAAAGACTT ATCCAGGAAC CTGACTTCAC AGCGGTCATT
    TCTTGGTCCA TTCGAAGAAA TGTGAGTCTT GGAGCTAAGG CAGAGGTAAC AGGTGAAAAA
    TATACAATAT CACTGGCAGG TCAAACTCGG AGGGAACTTG CGGATATGAT GAATAGTTCA
    TCTCCCAATA AGACAGTGGA ACTGATAAAG ATATACCCAT TGTGTTTAAA AGCACCAAGT
    GATGTTACTG CAAAACCCAT CCTTCAGCTT CTTAAGAATC AAGAGTTTGT GAATATTAGC
    GTTTTACTAA GAAAAGAAAA CACATCAGAA GGGGTTCGAG AATGGTGGGT AGTGAATCAA
    AGCCATAAAG AGTTCATTAA GAAGGATGGC CTGGAACTAT GGGTTTTCAG TGACAAAGTC
    AGTCCTCCAA GTCTTGGATT CTTGGCAGGC TATGGAATAA TGGGACTATA TGCCTCTGTA
    GTTCTCGTAA TAGGAAAGTT TGTGCGTGAG TTTTTCAGCG GAATTTCGCA CTCCATCATG
    TTTGAGGAGC TTCCCAACGT TGACCGTATA CTGAAGCTGT GCACAGACAT TTTCCTTGTG
    AGAGAGACTG GAGAGTTGGA ACTGGAGGAA GACCTGTATG CCAAATTAAT ATTTTTGTAC
    CGCTCCCCAG AAACTATGAT CAAATGGACA AGAGAGAAAG ATAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa60023
    Clone ID Related Accession (Same CDS sequence) XM_031903663.1
    Accession Version XM_031903663.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8337bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product piezo-type mechanosensitive ion channel component 2 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    ATGGCATCGG AAGTGGTGTG TGGGCTCATC TTCAGATTTC TTCTCCCACT ATGCCTGGTT 
    GTAGCGTGCA TGTTCCGGTA CAATGGAATA TCTTTTGTCT ACCTCATCTA CCTCTTGCTT
    ATACCTTTAT TTTCGGAGCC TACCAAAATA ACAATGCAAG GTCATACAGG ACGGTTATTA
    AAATCCTTAT GTGTTTCCAG TCTGTCATTT CTGCTGCTGC ACATCATCTT TCAAATTACC
    GTATACAGCC TCGAGGCCAG AAAACACATT GAACCACATT TTAACTGTTC AACATGGGAG
    AAAGCAATAC GACAAATTGG TTTTGAAAGT GTAAAAGGAG CAGATGCTGG TAATGGAATC
    CGCGTGTTTG CACCAGATAT CGGAATGTTT ATTGCAAGCT TGGCTACCCT GTTGGTTTGT
    AGAAAACTAG TTCAGAAAAC AGTTACTGAA GAGGACGCAC AATACAACCC CCAGTTTGTA
    AATGAAGAAA TGGCAGAAGA AGGAAAGGTG GACCTGGATG AAGCGTTAAT GTATGAGGAA
    GACTTTGATG GAGAAGAGGA TGAAATTGAG GCAGCTGAAG AAAGCACAAA ATTAAAAATT
    CTTCGCAGAA TTGCTTCATT TGCATCCGTA CTTAAAGAGT TTATTGGCAA CATTATAACC
    ACAGCTGGGA AAGTAGTTAT TACAATTTTA CTTGGTTTAA CAGGCATGAT GTTGCCGTCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT TTGGGGCTAT GCACATGGTG GTCCTGTTGC
    CGAGTATTTG ATCCGCTGAT ATTCAGCTGT CTATGTGTAT TAATGGCTAT ATTTAGTGCA
    GGGCACTTAA TTGGACTTTA TCTGTACCAG TTGCAGTTTT TTCACGAGAT TATTCCACCA
    AATGATTTCT ATGCCAGGTT GTTTGGTGTT ACTTCATTAG TTCAAACCGA CTGCTGTAAC
    ACTTGGAGAT TTTTTGCAAA CAACAGTCTA CCATGGAACC AGTATACCAA TCCTGTCATG
    CTTTTAGTAC TGTATTACAC GCTGGCAACT CTCATACGCC TTTGGCTACA GGAACCTATT
    AATGTAGAGG AAGAGAAGGA GGAAGACTGT AGCCCAGCAG CACTTACTGC AGAAAGAAGA
    CGTAGTTTGT GGTACTCTAG GCACTACACA ACGGATGAAA GACAACTTCT GTCCATGACT
    CAAGATGACT ACAAGCCATC TGATGTTTTA CTAGTAACTG TAAATGGGAC TCCCGTAGAT
    TACTGTTCAC TAAATTCATC ACTTCCGATA GAAAATGGTC CAACTAAAAT TGACATGTAT
    TCAACACCGC AATATAAATG GGACCATGAA GATGAAACTT CAGAAAAGAA AGAAGAGGAA
    GAGCAAGAAG AAGAACCCAA TGAAGAGGTT GCAGAAGAGA AAGAAAACAC TAAAGTTCAT
    GTAATGGTGG TGGCATTTCA GTTCATTATG AAGCAAAGCT ATATATGTGC ACTAATTTCA
    ATGATGGCCT GGAGCATCAC ATACCACAGC TGGTTGACTT TTGTTTTGTT AATCTGGTCT
    TGTGCTCTCT GGATGGTCCG AAATAGGAGA AAATATGCTA TGCTTAGTTC ACCTTTTATG
    GTTATGTATG GTAATTTACT TTTAACATTA CAATACATAT GGAGCATAGC CTTACAAAGT
    GATGAACTTC CAGAAGTTAT GGGTCTCCTT GAGAGAAAAA AGCCCGAAGA GCTTGTATCG
    AAGATCATTT TCACAGTTAC ATTTTGGCTT TTGCTCAGAC AACACCTTAC AGAGCAAAAG
    GCATTAAAGC AAAAAGAATT AGCATTATCT GAAATAAAAG TTGGAAGTGA AGACAAAGAA
    GGTGAAGTGC AAGAAGAAGA AGAAGAAGTG GAGGATGAAA ATGATATCAT GAAGGTCCTC
    GGAAAGCTAG TGCAGGCTAT GTTTGTCAAG TACTGGATTT ATGTCTGTGG CGGCATGTTT
    TTTTTTGTCA GCTTTGAAGG AAAAATAGTT ATGTACAAAA TTATCTACAT GATGCTCTTC
    CTATTCTGTG TAGCTCTGTA TCAGGTGCAC TATGAATGGT GGAGACGAAT TTTAAAATAT
    TTTTGGATGT CTGTGGTTGT TTACACCATG TTGGTGCTTG TTTTTATTTA CACTTACCAA
    TTTAAATCAT TTCCGGGCCT TTGGATGAAT ATGACAGGGC TGGATAAGGA AAAGTTAGCA
    GATCTTGGTT TAGAGACATT TAACTTGACT GACCTATTTA CTCGTATATT TATCCCAACC
    TCATTTTTGT TAGTGTGCAT TTTGCACCTA CATTACTTCC ATGACCGTTT TCTCCAGCTC
    ACTGACCTAA AGGCTGTAAC CAGCAAGCAG GACAGCACAA TTTACAGCCA TGCCAAAGTA
    AATGGTCGAG TTTACTTAAT AATAAATAGA TTGGTGCATC CAGAAGGAAG TTTGCCTGAT
    ATAACGATGA TGAACTTAAC AACAGAATCT GAAATGCTTG AGGAAAAGCG GTTGGAAATT
    TATGACGAAA AAAAAACTCA TGACCAAGAC TCAGATGATG AATGGGATAA AGGGGATAGC
    AAAGAACCAG GTCATTCTGA TGATGAAGCT GAGGATGAGG AATCAGAAGA AGAAGAAACA
    ACAACAGAGT CCAGGAACAA GTGGCATCTA GTGATAGATC GCTTAACGGT TCTTTTCCTA
    AAGTTTCTGG AATATTTTCA CAAGTTTCAG GTCTTTGTTT GGTGGCTTCT GGAGCTTCAC
    ATGATAAAGA TTGTTTCTTC ATACATCATT TGGCTTACTA TAAAGGAGGT TTCACTGCTC
    AATTATGTAT TTTTTATTGC CTGGGCCTTC GCTTTGCCCT ACTCACAATT CCGGCCATTG
    GCATCAAGTG TTTGCACAGT CTGGACATGT GTCATCATCA TATGTAAAAT GTTGTACCAG
    CTAGCCTCCA TTGACCCTGC AACCTTTTCC AGCAACTGTT TAATGCCACA GCTAAACGAA
    ACAACTGCAC GTAACCAAAC TGAGCTTAAT TCTTCTGTGC TTTACAATTC CTCTGTTGAT
    CCTTCTAATT GGGTTGGACT AAAGAAGTCA ACTCCACTCC TTGCTTATTT GCGGAACAAT
    TTGCTGATGT TGGCCATGTT AGCTTTTGAA GTAACCATTT ACCGGCATCA GGAGTATTAC
    AGGTTGCGAA ACAATCTCAC CACACCTGCA ACAAAAACCA TCTTTCATGA CATCACAAGA
    CTTCACTTGG ATGATGGACT CATAAATAGT GCCAAGTATT TTGTGAACTA CTTTTTCTAC
    AAGTTTGGAT TGGAAACATG TTTCTTACTG TCGGTAAATG TAATCGGTCA GAGGATGGAC
    TTTTTTGCCA TGGTTCATGC ACTTTGGCTT TTTGGCGTAT TATACCGACG CAGAAGGAAA
    GCGATTGCAG AAACCTGGCA CAGATATTGC TGTTTTCTTG CTTGCATTAT GGCATTCCAG
    TATTTTATAT GTATTGGCAT CCCCCCTGCT GCATGTAAAG ACTATCCTTG GAGACAACCT
    GGAGCCAATT TTAATTCTAA CATAATAAAG TGGTTATACT TTCCAGACTT TATTGTGAGG
    CCTAGTCCTC TGTTTCTTGT CTATGATTTT TTGCTTTTGC TTTGTGCGTC CCTTCAAAGA
    GAAGTGTTTG AGGAAGAAAA TAAGGCAGCA GTTCGAATCA TGGCTGGTGA CAATGTAGAA
    ATTTGCATGA ACCTTGATGC TGCATCTTTT AGCCAACATA ATCCTGTACC TGATTTCATA
    TACTGCAGAT CTTATTTGGA CATGGGTAAA GTTATAATGT TTAGTTACCT TTTCTGGTTT
    GTGCTCACCA TCATCTTCAT CACGGGAACT ACACGGATCA GCATATTCTG CATGGGCTAC
    TTGGTAGCTT GCTTCTATTT TCTATTATTT GGTGGAGATT TGCTGTTAAA ACCTATAAGA
    ATCATCCTAC GCTATTGGGA TTGGCTGATT GCTTACAATG TTTTTGTCAT TACAATGAAA
    AATGTATTAT CAATAGGAGC ATGTGGCTAC ATTGAATATT TAATCAAAGA CAGTTGTTGG
    TTAATTCAGG CATTCAGTCT TTCTTGTACA GTGAAAGGTT ATTTTATCCC CCAAAACACA
    CCTGACTGTC AGTTGCCAAG CGGAGAGGCT GGAATCATTT GGGACAGTAT ATGTTTTGCT
    TTCCTTTTAT TGCAAAGAAG AGTGTTTATG AGCTATTACT TCTTACATGT GGTTGCAGAC
    ATCAAATCAT CTCAGATTCT AGCATCAAGA GGTGCTGAAC TGTTCCAAGC TACAATTGTA
    AAAGCTGTAA AAGCAAGAAT TGAAGAAGAA AAAAAATCTA TGGACCAGCT CAAGAGACAG
    ATGGACCACA TTAAAGCCAG GCAAGAAAAA TATAAAAAAG GTAAAGAAAA AATGCTGAGT
    CTTACACAGG ATGGAGAAGG ACAAGAAGTT CAAAAACTAG ATGATGACGA TGAAGAAGGA
    GACAAAGCAG ACAAAGAGAA AGCCAAGGGC AAAAAAAAGC AGTGGTGGCG ACCTTGGGTT
    GATCATGCTT CCATGGTGAA AAGCGGTGAT TATTATTTGT TTGAGACGGA CAGTGAAGAA
    GAAGATGAGG ATGACAAAAA AGAAGAAGAG CCTAGAAGAA AATCTGCCTT CCAGTTTTTG
    TACCAAGCAT GGATTACTGA TCCAAAAACA GCCCTTAAGC AAAGAAGCAT GGAAGCCAAA
    GAAGAAAAGG CAAGGGAAAT GAGGCGAAAG GGCATTGCTG AAGGTGATAT TATTGTAGAA
    TGTGGGGATA AAGAAGAAGA AAAAGAGCAA GGAAAAAAGA AATCAGATGG CCCAGACAAT
    ATCTTCAAAA GAATATTTAA CATCCTGAAG TTTACTTGGG TCCTCTTCCT TGCTACAATC
    GATAGTTTTA CGGCTTGGCT AAACTCCATT TCAAGAGAAC ATATTGATAT CTCCAAAGTT
    CTAAGCATAG AACGTTGCAT GCTGACTAGA GAGATAAAAA AGGGCAATGT GCCTACTCGA
    GAAAGCATCC ACATGTATTA CCAAAATCAA ATAATGATGA GCCTTTCTAG AGAGTCTGGA
    CTTGACAGAA TTGATGACCA ACCTGGTTCA TCGTCCAGTC ACCATAGCCA TGACAAGATG
    GCGAGTAAAA GGATGGACAG TGCAGACTCA GCTGTATCTC ATGATAGCAT ATCAAGCTGC
    TGCACTGAAG CAACCATGCT ATTCTCAAGG CAATCTACAC TTGATGATTT AGATGGACCA
    GACTCTGTAC CAAAAACAAG TGAGCGTGCT CGACCTAGGC TTCGTAAAAT GTACAGCATG
    GACATGTCTT CTTCATCTGC TGACAGTATA GCTTCAAGTG AGCCAACTCA GTGCACCATG
    GTCTACTCAC GGCAGGGCAC AACAGAAACT ATTGAGGAAG TGGAAGATGA AGAGGAGGAA
    GAAAGCAAAA GTCCTTTATC AAAGATAGAA GCCGAGGAAG AAGAATATGA CATGGATTTG
    GAAGGGACAA GCTATACTCC GGACACAGAA TTTCCCCTGT ACGCCACTAC AGAGGGGGAT
    CTTCCTCCTT CGTACAGTAA AGCCGGCAGC TTCGAACAAC TTACATTTGT ATCTCAGGAC
    TCGGCTGACA ATTTAATGGT TCTAAGCCCA GATGAAAGTC GTACTGATAA GCTACATGAT
    CATATTCTTC CACCTCTTAC ACATGAACTG ACTGCTAGCG AGCTGCTTCT AAACAAAATG
    TTTTATGATG AAGAGTTAGA AGACTCTGAA AAATTTTATA CTACTCAACC ACGCCCGCTG
    CAGCTTATTT ATGCCTTGTA TAATACATTG GTGGCCAGAT CAGAAATGGT GTGCTATTTT
    GTGATTATTC TTAATCACAT GATTTCAGCT TCTATGATCA CATTGGTACT GCCCATCCTT
    ATATTCCTTT GGGCTATGCT TTCTGTCCCA AGGCCCAGTA AGAGATTTTG GATGACAGCT
    ATTGTATATA CAGAGGTGAC AATTGTTATT AAATATTTTT TCCAATTTGG ATTCTTCCCA
    TGGAATAAAA ATGTGGATTA TACAAAGGAC AAACCTTACT TCCCACCTAA CATAATGGGC
    ATTGAGAAGA AAGAAGGTTA TGTGCACTAT GACCTGGTGC AACTGCTTGC TCTGTTTTTC
    CATAGATCTA TTTTAAAGTG CCACGGGCTT TGGGATGAAG ATGATGTCAC TGAAAAAGCC
    ACAGATAAAG ACGACACGGA TGGAGAGATA TCAGAGGCAT CAGGAAGAAG GGATTCCGTT
    GGCTCATTGA AATCTGTCAA TCTAGCTGCG TCTGTGGAGT CAGTCCATGT GCACTTCCCT
    GAGGAAAAGA CCGCTATCAG GAGAAAAAGC ACAAGCAGTG GATCGCAAAT TTCCCGAAGG
    TCAAGTTATT CCTCGCACAG GTCTAAAAGA GGCAGCACCA GTACTCGGAA CAGCAGTCAA
    AAAGGAAGCA GCATTCCCAG TATTAAACTG AAATCTAGAA AGGAACTTAT ACTGGAAAAA
    CTCAGAGAAC AGCTCATTAA AGCAAAAGCA TTTACGATCA AGACGATACT ACAAATCTAT
    GTCCCGATCA GACAGTTTTT TTACAATCTT ATACATCCTG AATACAGTGC TGTGACTGAT
    GTCTATGTGC TGATGTTCCT GGCAGACACT GTAGATTTCA TCATTATTGT TTTTGGATTT
    TGGGCGTTTG GAAAACACTC CGCTGCAGCA GATATTACAT CCTCTCTATC AGAAGACCAA
    GTCCCAGAAG CATTTTTGGT AATGGTGCTT ATCCAGTTTG GAACCATGGT TGTTGATCGT
    GCTTTGTATC TTCGCAAAAC AGTCATGGGA AAAGTTATTT TCCAAGTTAT CCTTGTATTT
    GGAATCCATT TTTGGATGTT CTTCATCTTG CCAGGGGTAA CAGAAAGGAA ATTTAGTCAG
    AACACTGTTG CCCAACTGTG GTATTTTGTC AAGTGCATTT ATTTTGGATT GTCGGCATAT
    CAGATACGTT GTGGATATCC AACTCGGGTG CTTGGTAACT TCCTTACTAA AAGCTACAAT
    TATGTCAACC TGTTCCTTTT CCAAGGCTTT CGCCTCGTTC CATTTCTTAC GGAGCTGCGA
    GCTGTTATGG ACTGGGTATG GACTGACACC ACTCTCAGTC TTTCCAGCTG GATCTGTGTA
    GAGGACATTT ATGCACATAT ATTTATACTA AAATGCTGGC GGGAGTCAGA AAAGAGATAT
    CCCCAGCCAC GAGGCCAAAA GAAGAAAAAG GTTGTGAAAT ACGGAATGGG TGGAATGATA
    ATAGTTCTGC TGATTTGTAT CGTGTGGTTT CCTTTGCTCT TCATGTCCTT GATTAAATCA
    GTTGCTGGGG TCACTAACAA GCCCCTAGAT GTGTCCATTA CAATAACCCT TGGAGGTTAT
    CAGCCTATTT TTACCATGAG TGCACAACAA AATCAGCTGC AAGGCTTGAA TGACAATGAA
    TTTAAGCATT TACATAATAT ATATAAAGGC AATACACCTG CCATGCAGTT TCTGGAATCC
    TATATGCAGG AAGACATCAC CATAGCAGGA CTGGAAGGCA ATTCCAACTC TCTGTGGACC
    ATCAGCCCTC CGAGCAGGAC AATGATGATA GAAAGACTTA TCCAGGAACC TGACTTCACA
    GCGGTCATTT CTTGGTCCAT TCGAAGAAAT GTGAGTCTTG GAGCTAAGGC AGAGGTAACA
    GGTGAAAAAT ATACAATATC ACTGGCAGGT CAAACTCGGA GGGAACTTGC GGATATGATG
    AATAGTTCAT CTCCCAATAA GACAGTGGAA CTGATAAAGA TATACCCATT GTGTTTAAAA
    GCACCAAGTG ATGTTACTGC AAAACCCATC CTTCAGCTTC TTAAGAATCA AGAGTTTGTG
    AATATTAGCG TTTTACTAAG AAAAGAAAAC ACATCAGAAG GGGTTCGAGA ATGGTGGGTA
    GTGAATCAAA GCCATAAAGA GTTCATTAAG AAGGATGGCC TGGAACTATG GGTTTTCAGT
    GACAAAGTCA GTCCTCCAAG TCTTGGATTC TTGGCAGGCT ATGGAATAAT GGGACTATAT
    GCCTCTGTAG TTCTCGTAAT AGGAAAGTTT GTGCGTGAGT TTTTCAGCGG AATTTCGCAC
    TCCATCATGT TTGAGGAGCT TCCCAACGTT GACCGTATAC TGAAGCTGTG CACAGACATT
    TTCCTTGTGA GAGAGACTGG AGAGTTGGAA CTGGAGGAAG ACCTGTATGC CAAATTAATA
    TTTTTGTACC GCTCCCCAGA AACTATGATC AAATGGACAA GAGAGAAAGA TAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031759523.1
    CDS632..8968
    Translation

    Target ORF information:

    RefSeq Version XM_031903663.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis piezo-type mechanosensitive ion channel component 2 (piezo2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031903663.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    ATGGCATCGG AAGTGGTGTG TGGGCTCATC TTCAGATTTC TTCTCCCACT ATGCCTGGTT 
    GTAGCGTGCA TGTTCCGGTA CAATGGAATA TCTTTTGTCT ACCTCATCTA CCTCTTGCTT
    ATACCTTTAT TTTCGGAGCC TACCAAAATA ACAATGCAAG GTCATACAGG ACGGTTATTA
    AAATCCTTAT GTGTTTCCAG TCTGTCATTT CTGCTGCTGC ACATCATCTT TCAAATTACC
    GTATACAGCC TCGAGGCCAG AAAACACATT GAACCACATT TTAACTGTTC AACATGGGAG
    AAAGCAATAC GACAAATTGG TTTTGAAAGT GTAAAAGGAG CAGATGCTGG TAATGGAATC
    CGCGTGTTTG CACCAGATAT CGGAATGTTT ATTGCAAGCT TGGCTACCCT GTTGGTTTGT
    AGAAAACTAG TTCAGAAAAC AGTTACTGAA GAGGACGCAC AATACAACCC CCAGTTTGTA
    AATGAAGAAA TGGCAGAAGA AGGAAAGGTG GACCTGGATG AAGCGTTAAT GTATGAGGAA
    GACTTTGATG GAGAAGAGGA TGAAATTGAG GCAGCTGAAG AAAGCACAAA ATTAAAAATT
    CTTCGCAGAA TTGCTTCATT TGCATCCGTA CTTAAAGAGT TTATTGGCAA CATTATAACC
    ACAGCTGGGA AAGTAGTTAT TACAATTTTA CTTGGTTTAA CAGGCATGAT GTTGCCGTCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT TTGGGGCTAT GCACATGGTG GTCCTGTTGC
    CGAGTATTTG ATCCGCTGAT ATTCAGCTGT CTATGTGTAT TAATGGCTAT ATTTAGTGCA
    GGGCACTTAA TTGGACTTTA TCTGTACCAG TTGCAGTTTT TTCACGAGAT TATTCCACCA
    AATGATTTCT ATGCCAGGTT GTTTGGTGTT ACTTCATTAG TTCAAACCGA CTGCTGTAAC
    ACTTGGAGAT TTTTTGCAAA CAACAGTCTA CCATGGAACC AGTATACCAA TCCTGTCATG
    CTTTTAGTAC TGTATTACAC GCTGGCAACT CTCATACGCC TTTGGCTACA GGAACCTATT
    AATGTAGAGG AAGAGAAGGA GGAAGACTGT AGCCCAGCAG CACTTACTGC AGAAAGAAGA
    CGTAGTTTGT GGTACTCTAG GCACTACACA ACGGATGAAA GACAACTTCT GTCCATGACT
    CAAGATGACT ACAAGCCATC TGATGTTTTA CTAGTAACTG TAAATGGGAC TCCCGTAGAT
    TACTGTTCAC TAAATTCATC ACTTCCGATA GAAAATGGTC CAACTAAAAT TGACATGTAT
    TCAACACCGC AATATAAATG GGACCATGAA GATGAAACTT CAGAAAAGAA AGAAGAGGAA
    GAGCAAGAAG AAGAACCCAA TGAAGAGGTT GCAGAAGAGA AAGAAAACAC TAAAGTTCAT
    GTAATGGTGG TGGCATTTCA GTTCATTATG AAGCAAAGCT ATATATGTGC ACTAATTTCA
    ATGATGGCCT GGAGCATCAC ATACCACAGC TGGTTGACTT TTGTTTTGTT AATCTGGTCT
    TGTGCTCTCT GGATGGTCCG AAATAGGAGA AAATATGCTA TGCTTAGTTC ACCTTTTATG
    GTTATGTATG GTAATTTACT TTTAACATTA CAATACATAT GGAGCATAGC CTTACAAAGT
    GATGAACTTC CAGAAGTTAT GGGTCTCCTT GAGAGAAAAA AGCCCGAAGA GCTTGTATCG
    AAGATCATTT TCACAGTTAC ATTTTGGCTT TTGCTCAGAC AACACCTTAC AGAGCAAAAG
    GCATTAAAGC AAAAAGAATT AGCATTATCT GAAATAAAAG TTGGAAGTGA AGACAAAGAA
    GGTGAAGTGC AAGAAGAAGA AGAAGAAGTG GAGGATGAAA ATGATATCAT GAAGGTCCTC
    GGAAAGCTAG TGCAGGCTAT GTTTGTCAAG TACTGGATTT ATGTCTGTGG CGGCATGTTT
    TTTTTTGTCA GCTTTGAAGG AAAAATAGTT ATGTACAAAA TTATCTACAT GATGCTCTTC
    CTATTCTGTG TAGCTCTGTA TCAGGTGCAC TATGAATGGT GGAGACGAAT TTTAAAATAT
    TTTTGGATGT CTGTGGTTGT TTACACCATG TTGGTGCTTG TTTTTATTTA CACTTACCAA
    TTTAAATCAT TTCCGGGCCT TTGGATGAAT ATGACAGGGC TGGATAAGGA AAAGTTAGCA
    GATCTTGGTT TAGAGACATT TAACTTGACT GACCTATTTA CTCGTATATT TATCCCAACC
    TCATTTTTGT TAGTGTGCAT TTTGCACCTA CATTACTTCC ATGACCGTTT TCTCCAGCTC
    ACTGACCTAA AGGCTGTAAC CAGCAAGCAG GACAGCACAA TTTACAGCCA TGCCAAAGTA
    AATGGTCGAG TTTACTTAAT AATAAATAGA TTGGTGCATC CAGAAGGAAG TTTGCCTGAT
    ATAACGATGA TGAACTTAAC AACAGAATCT GAAATGCTTG AGGAAAAGCG GTTGGAAATT
    TATGACGAAA AAAAAACTCA TGACCAAGAC TCAGATGATG AATGGGATAA AGGGGATAGC
    AAAGAACCAG GTCATTCTGA TGATGAAGCT GAGGATGAGG AATCAGAAGA AGAAGAAACA
    ACAACAGAGT CCAGGAACAA GTGGCATCTA GTGATAGATC GCTTAACGGT TCTTTTCCTA
    AAGTTTCTGG AATATTTTCA CAAGTTTCAG GTCTTTGTTT GGTGGCTTCT GGAGCTTCAC
    ATGATAAAGA TTGTTTCTTC ATACATCATT TGGCTTACTA TAAAGGAGGT TTCACTGCTC
    AATTATGTAT TTTTTATTGC CTGGGCCTTC GCTTTGCCCT ACTCACAATT CCGGCCATTG
    GCATCAAGTG TTTGCACAGT CTGGACATGT GTCATCATCA TATGTAAAAT GTTGTACCAG
    CTAGCCTCCA TTGACCCTGC AACCTTTTCC AGCAACTGTT TAATGCCACA GCTAAACGAA
    ACAACTGCAC GTAACCAAAC TGAGCTTAAT TCTTCTGTGC TTTACAATTC CTCTGTTGAT
    CCTTCTAATT GGGTTGGACT AAAGAAGTCA ACTCCACTCC TTGCTTATTT GCGGAACAAT
    TTGCTGATGT TGGCCATGTT AGCTTTTGAA GTAACCATTT ACCGGCATCA GGAGTATTAC
    AGGTTGCGAA ACAATCTCAC CACACCTGCA ACAAAAACCA TCTTTCATGA CATCACAAGA
    CTTCACTTGG ATGATGGACT CATAAATAGT GCCAAGTATT TTGTGAACTA CTTTTTCTAC
    AAGTTTGGAT TGGAAACATG TTTCTTACTG TCGGTAAATG TAATCGGTCA GAGGATGGAC
    TTTTTTGCCA TGGTTCATGC ACTTTGGCTT TTTGGCGTAT TATACCGACG CAGAAGGAAA
    GCGATTGCAG AAACCTGGCA CAGATATTGC TGTTTTCTTG CTTGCATTAT GGCATTCCAG
    TATTTTATAT GTATTGGCAT CCCCCCTGCT GCATGTAAAG ACTATCCTTG GAGACAACCT
    GGAGCCAATT TTAATTCTAA CATAATAAAG TGGTTATACT TTCCAGACTT TATTGTGAGG
    CCTAGTCCTC TGTTTCTTGT CTATGATTTT TTGCTTTTGC TTTGTGCGTC CCTTCAAAGA
    GAAGTGTTTG AGGAAGAAAA TAAGGCAGCA GTTCGAATCA TGGCTGGTGA CAATGTAGAA
    ATTTGCATGA ACCTTGATGC TGCATCTTTT AGCCAACATA ATCCTGTACC TGATTTCATA
    TACTGCAGAT CTTATTTGGA CATGGGTAAA GTTATAATGT TTAGTTACCT TTTCTGGTTT
    GTGCTCACCA TCATCTTCAT CACGGGAACT ACACGGATCA GCATATTCTG CATGGGCTAC
    TTGGTAGCTT GCTTCTATTT TCTATTATTT GGTGGAGATT TGCTGTTAAA ACCTATAAGA
    ATCATCCTAC GCTATTGGGA TTGGCTGATT GCTTACAATG TTTTTGTCAT TACAATGAAA
    AATGTATTAT CAATAGGAGC ATGTGGCTAC ATTGAATATT TAATCAAAGA CAGTTGTTGG
    TTAATTCAGG CATTCAGTCT TTCTTGTACA GTGAAAGGTT ATTTTATCCC CCAAAACACA
    CCTGACTGTC AGTTGCCAAG CGGAGAGGCT GGAATCATTT GGGACAGTAT ATGTTTTGCT
    TTCCTTTTAT TGCAAAGAAG AGTGTTTATG AGCTATTACT TCTTACATGT GGTTGCAGAC
    ATCAAATCAT CTCAGATTCT AGCATCAAGA GGTGCTGAAC TGTTCCAAGC TACAATTGTA
    AAAGCTGTAA AAGCAAGAAT TGAAGAAGAA AAAAAATCTA TGGACCAGCT CAAGAGACAG
    ATGGACCACA TTAAAGCCAG GCAAGAAAAA TATAAAAAAG GTAAAGAAAA AATGCTGAGT
    CTTACACAGG ATGGAGAAGG ACAAGAAGTT CAAAAACTAG ATGATGACGA TGAAGAAGGA
    GACAAAGCAG ACAAAGAGAA AGCCAAGGGC AAAAAAAAGC AGTGGTGGCG ACCTTGGGTT
    GATCATGCTT CCATGGTGAA AAGCGGTGAT TATTATTTGT TTGAGACGGA CAGTGAAGAA
    GAAGATGAGG ATGACAAAAA AGAAGAAGAG CCTAGAAGAA AATCTGCCTT CCAGTTTTTG
    TACCAAGCAT GGATTACTGA TCCAAAAACA GCCCTTAAGC AAAGAAGCAT GGAAGCCAAA
    GAAGAAAAGG CAAGGGAAAT GAGGCGAAAG GGCATTGCTG AAGGTGATAT TATTGTAGAA
    TGTGGGGATA AAGAAGAAGA AAAAGAGCAA GGAAAAAAGA AATCAGATGG CCCAGACAAT
    ATCTTCAAAA GAATATTTAA CATCCTGAAG TTTACTTGGG TCCTCTTCCT TGCTACAATC
    GATAGTTTTA CGGCTTGGCT AAACTCCATT TCAAGAGAAC ATATTGATAT CTCCAAAGTT
    CTAAGCATAG AACGTTGCAT GCTGACTAGA GAGATAAAAA AGGGCAATGT GCCTACTCGA
    GAAAGCATCC ACATGTATTA CCAAAATCAA ATAATGATGA GCCTTTCTAG AGAGTCTGGA
    CTTGACAGAA TTGATGACCA ACCTGGTTCA TCGTCCAGTC ACCATAGCCA TGACAAGATG
    GCGAGTAAAA GGATGGACAG TGCAGACTCA GCTGTATCTC ATGATAGCAT ATCAAGCTGC
    TGCACTGAAG CAACCATGCT ATTCTCAAGG CAATCTACAC TTGATGATTT AGATGGACCA
    GACTCTGTAC CAAAAACAAG TGAGCGTGCT CGACCTAGGC TTCGTAAAAT GTACAGCATG
    GACATGTCTT CTTCATCTGC TGACAGTATA GCTTCAAGTG AGCCAACTCA GTGCACCATG
    GTCTACTCAC GGCAGGGCAC AACAGAAACT ATTGAGGAAG TGGAAGATGA AGAGGAGGAA
    GAAAGCAAAA GTCCTTTATC AAAGATAGAA GCCGAGGAAG AAGAATATGA CATGGATTTG
    GAAGGGACAA GCTATACTCC GGACACAGAA TTTCCCCTGT ACGCCACTAC AGAGGGGGAT
    CTTCCTCCTT CGTACAGTAA AGCCGGCAGC TTCGAACAAC TTACATTTGT ATCTCAGGAC
    TCGGCTGACA ATTTAATGGT TCTAAGCCCA GATGAAAGTC GTACTGATAA GCTACATGAT
    CATATTCTTC CACCTCTTAC ACATGAACTG ACTGCTAGCG AGCTGCTTCT AAACAAAATG
    TTTTATGATG AAGAGTTAGA AGACTCTGAA AAATTTTATA CTACTCAACC ACGCCCGCTG
    CAGCTTATTT ATGCCTTGTA TAATACATTG GTGGCCAGAT CAGAAATGGT GTGCTATTTT
    GTGATTATTC TTAATCACAT GATTTCAGCT TCTATGATCA CATTGGTACT GCCCATCCTT
    ATATTCCTTT GGGCTATGCT TTCTGTCCCA AGGCCCAGTA AGAGATTTTG GATGACAGCT
    ATTGTATATA CAGAGGTGAC AATTGTTATT AAATATTTTT TCCAATTTGG ATTCTTCCCA
    TGGAATAAAA ATGTGGATTA TACAAAGGAC AAACCTTACT TCCCACCTAA CATAATGGGC
    ATTGAGAAGA AAGAAGGTTA TGTGCACTAT GACCTGGTGC AACTGCTTGC TCTGTTTTTC
    CATAGATCTA TTTTAAAGTG CCACGGGCTT TGGGATGAAG ATGATGTCAC TGAAAAAGCC
    ACAGATAAAG ACGACACGGA TGGAGAGATA TCAGAGGCAT CAGGAAGAAG GGATTCCGTT
    GGCTCATTGA AATCTGTCAA TCTAGCTGCG TCTGTGGAGT CAGTCCATGT GCACTTCCCT
    GAGGAAAAGA CCGCTATCAG GAGAAAAAGC ACAAGCAGTG GATCGCAAAT TTCCCGAAGG
    TCAAGTTATT CCTCGCACAG GTCTAAAAGA GGCAGCACCA GTACTCGGAA CAGCAGTCAA
    AAAGGAAGCA GCATTCCCAG TATTAAACTG AAATCTAGAA AGGAACTTAT ACTGGAAAAA
    CTCAGAGAAC AGCTCATTAA AGCAAAAGCA TTTACGATCA AGACGATACT ACAAATCTAT
    GTCCCGATCA GACAGTTTTT TTACAATCTT ATACATCCTG AATACAGTGC TGTGACTGAT
    GTCTATGTGC TGATGTTCCT GGCAGACACT GTAGATTTCA TCATTATTGT TTTTGGATTT
    TGGGCGTTTG GAAAACACTC CGCTGCAGCA GATATTACAT CCTCTCTATC AGAAGACCAA
    GTCCCAGAAG CATTTTTGGT AATGGTGCTT ATCCAGTTTG GAACCATGGT TGTTGATCGT
    GCTTTGTATC TTCGCAAAAC AGTCATGGGA AAAGTTATTT TCCAAGTTAT CCTTGTATTT
    GGAATCCATT TTTGGATGTT CTTCATCTTG CCAGGGGTAA CAGAAAGGAA ATTTAGTCAG
    AACACTGTTG CCCAACTGTG GTATTTTGTC AAGTGCATTT ATTTTGGATT GTCGGCATAT
    CAGATACGTT GTGGATATCC AACTCGGGTG CTTGGTAACT TCCTTACTAA AAGCTACAAT
    TATGTCAACC TGTTCCTTTT CCAAGGCTTT CGCCTCGTTC CATTTCTTAC GGAGCTGCGA
    GCTGTTATGG ACTGGGTATG GACTGACACC ACTCTCAGTC TTTCCAGCTG GATCTGTGTA
    GAGGACATTT ATGCACATAT ATTTATACTA AAATGCTGGC GGGAGTCAGA AAAGAGATAT
    CCCCAGCCAC GAGGCCAAAA GAAGAAAAAG GTTGTGAAAT ACGGAATGGG TGGAATGATA
    ATAGTTCTGC TGATTTGTAT CGTGTGGTTT CCTTTGCTCT TCATGTCCTT GATTAAATCA
    GTTGCTGGGG TCACTAACAA GCCCCTAGAT GTGTCCATTA CAATAACCCT TGGAGGTTAT
    CAGCCTATTT TTACCATGAG TGCACAACAA AATCAGCTGC AAGGCTTGAA TGACAATGAA
    TTTAAGCATT TACATAATAT ATATAAAGGC AATACACCTG CCATGCAGTT TCTGGAATCC
    TATATGCAGG AAGACATCAC CATAGCAGGA CTGGAAGGCA ATTCCAACTC TCTGTGGACC
    ATCAGCCCTC CGAGCAGGAC AATGATGATA GAAAGACTTA TCCAGGAACC TGACTTCACA
    GCGGTCATTT CTTGGTCCAT TCGAAGAAAT GTGAGTCTTG GAGCTAAGGC AGAGGTAACA
    GGTGAAAAAT ATACAATATC ACTGGCAGGT CAAACTCGGA GGGAACTTGC GGATATGATG
    AATAGTTCAT CTCCCAATAA GACAGTGGAA CTGATAAAGA TATACCCATT GTGTTTAAAA
    GCACCAAGTG ATGTTACTGC AAAACCCATC CTTCAGCTTC TTAAGAATCA AGAGTTTGTG
    AATATTAGCG TTTTACTAAG AAAAGAAAAC ACATCAGAAG GGGTTCGAGA ATGGTGGGTA
    GTGAATCAAA GCCATAAAGA GTTCATTAAG AAGGATGGCC TGGAACTATG GGTTTTCAGT
    GACAAAGTCA GTCCTCCAAG TCTTGGATTC TTGGCAGGCT ATGGAATAAT GGGACTATAT
    GCCTCTGTAG TTCTCGTAAT AGGAAAGTTT GTGCGTGAGT TTTTCAGCGG AATTTCGCAC
    TCCATCATGT TTGAGGAGCT TCCCAACGTT GACCGTATAC TGAAGCTGTG CACAGACATT
    TTCCTTGTGA GAGAGACTGG AGAGTTGGAA CTGGAGGAAG ACCTGTATGC CAAATTAATA
    TTTTTGTACC GCTCCCCAGA AACTATGATC AAATGGACAA GAGAGAAAGA TAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa60024
    Clone ID Related Accession (Same CDS sequence) XM_012965365.3
    Accession Version XM_012965365.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8133bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product piezo-type mechanosensitive ion channel component 2 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012965365.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    ATGGCATCGG AAGTGGTGTG TGGGCTCATC TTCAGATTTC TTCTCCCACT ATGCCTGGTT 
    GTAGCGTGCA TGTTCCGGTA CAATGGAATA TCTTTTGTCT ACCTCATCTA CCTCTTGCTT
    ATACCTTTAT TTTCGGAGCC TACCAAAATA ACAATGCAAG GTCATACAGG ACGGTTATTA
    AAATCCTTAT GTGTTTCCAG TCTGTCATTT CTGCTGCTGC ACATCATCTT TCAAATTACC
    GTATACAGCC TCGAGGCCAG AAAACACATT GAACCACATT TTAACTGTTC AACATGGGAG
    AAAGCAATAC GACAAATTGG TTTTGAAAGT GTAAAAGGAG CAGATGCTGG TAATGGAATC
    CGCGTGTTTG CACCAGATAT CGGAATGTTT ATTGCAAGCT TGGCTACCCT GTTGGTTTGT
    AGAAAACTAG TTCAGAAAAC AGTTACTGAA GAGGACGCAC AATACAACCC CCAGTTTGTA
    AATGAAGAAA TGGCAGAAGA AGGAAAGGTG GACCTGGATG AAGCGTTAAT GTATGAGGAA
    GACTTTGATG GAGAAGAGGA TGAAATTGAG GCAGCTGAAG AAAGCACAAA ATTAAAAATT
    CTTCGCAGAA TTGCTTCATT TGCATCCGTA CTTAAAGAGT TTATTGGCAA CATTATAACC
    ACAGCTGGGA AAGTAGTTAT TACAATTTTA CTTGGTTTAA CAGGCATGAT GTTGCCGTCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT TTGGGGCTAT GCACATGGTG GTCCTGTTGC
    CGAGTATTTG ATCCGCTGAT ATTCAGCTGT CTATGTGTAT TAATGGCTAT ATTTAGTGCA
    GGGCACTTAA TTGGACTTTA TCTGTACCAG TTGCAGTTTT TTCACGAGAT TATTCCACCA
    AATGATTTCT ATGCCAGGTT GTTTGGTGTT ACTTCATTAG TTCAAACCGA CTGCTGTAAC
    ACTTGGAGAT TTTTTGCAAA CAACAGTCTA CCATGGAACC AGTATACCAA TCCTGTCATG
    CTTTTAGTAC TGTATTACAC GCTGGCAACT CTCATACGCC TTTGGCTACA GGAACCTATT
    AATGTAGAGG AAGAGAAGGA GGAAGACTGT AGCCCAGCAG CACTTACTGC AGAAAGAAGA
    CGTAGTTTGT GGTACTCTAG GCACTACACA ACGGATGAAA GACAACTTCT GTCCATGACT
    CAAGATGACT ACAAGCCATC TGATGTTTTA CTAGTAACTG TAAATGGGAC TCCCGTAGAT
    TACTGTTCAC TAAATTCATC ACTTCCGATA GAAAATGGTC CAACTAAAAT TGACATGTAT
    TCAACACCGC AATATAAATG GGACCATGAA GATGAAACTT CAGAAAAGAA AGAAGAGGAA
    GAGCAAGAAG AAGAACCCAA TGAAGAGGTT GCAGAAGAGA AAGAAAACAC TAAAGTTCAT
    GTAATGGTGG TGGCATTTCA GTTCATTATG AAGCAAAGCT ATATATGTGC ACTAATTTCA
    ATGATGGCCT GGAGCATCAC ATACCACAGC TGGTTGACTT TTGTTTTGTT AATCTGGTCT
    TGTGCTCTCT GGATGGTCCG AAATAGGAGA AAATATGCTA TGCTTAGTTC ACCTTTTATG
    GTTATGTATG GTAATTTACT TTTAACATTA CAATACATAT GGAGCATAGC CTTACAAAGT
    GATGAACTTC CAGAAGTTAT GGGTCTCCTT GAGAGAAAAA AGCCCGAAGA GCTTGTATCG
    AAGATCATTT TCACAGTTAC ATTTTGGCTT TTGCTCAGAC AACACCTTAC AGAGCAAAAG
    GCATTAAAGC AAAAAGAATT AGCATTATCT GAAATAAAAG TTGGAAGTGA AGACAAAGAA
    GGTGAAGTGC AAGAAGAAGA AGAAGAAGTG GAGGATGAAA ATGATATCAT GAAGGTCCTC
    GGAAAGCTAG TGCAGGCTAT GTTTGTCAAG TACTGGATTT ATGTCTGTGG CGGCATGTTT
    TTTTTTGTCA GCTTTGAAGG AAAAATAGTT ATGTACAAAA TTATCTACAT GATGCTCTTC
    CTATTCTGTG TAGCTCTGTA TCAGGTGCAC TATGAATGGT GGAGACGAAT TTTAAAATAT
    TTTTGGATGT CTGTGGTTGT TTACACCATG TTGGTGCTTG TTTTTATTTA CACTTACCAA
    TTTAAATCAT TTCCGGGCCT TTGGATGAAT ATGACAGGGC TGGATAAGGA AAAGTTAGCA
    GATCTTGGTT TAGAGACATT TAACTTGACT GACCTATTTA CTCGTATATT TATCCCAACC
    TCATTTTTGT TAGTGTGCAT TTTGCACCTA CATTACTTCC ATGACCGTTT TCTCCAGCTC
    ACTGACCTAA AGGCTGTAAC CAGCAAGCAG GACAGCACAA TTTACAGATT GGTGCATCCA
    GAAGGAAGTT TGCCTGATAT AACGATGATG AACTTAACAA CAGAATCTGA AATGCTTGAG
    GAAAAGCGGT TGGAAATTTA TGACGAAAAA AAAACTCATG ACCAAGACTC AGATGATGAA
    TGGGATAAAG GGGATAGCAA AGAACCAGGT CATTCTGATG ATGAAGCTGA GGATGAGGAA
    TCAGAAGAAG AAGAAACAAC AACAGAGTCC AGGAACAAGT GGCATCTAGT GATAGATCGC
    TTAACGGTTC TTTTCCTAAA GTTTCTGGAA TATTTTCACA AGTTTCAGGT CTTTGTTTGG
    TGGCTTCTGG AGCTTCACAT GATAAAGATT GTTTCTTCAT ACATCATTTG GCTTACTATA
    AAGGAGGTTT CACTGCTCAA TTATGTATTT TTTATTGCCT GGGCCTTCGC TTTGCCCTAC
    TCACAATTCC GGCCATTGGC ATCAAGTGTT TGCACAGTCT GGACATGTGT CATCATCATA
    TGTAAAATGT TGTACCAGCT AGCCTCCATT GACCCTGCAA CCTTTTCCAG CAACTGTTTA
    ATGCCACAGC TAAACGAAAC AACTGCACGT AACCAAACTG AGCTTAATTC TTCTGTGCTT
    TACAATTCCT CTGTTGATCC TTCTAATTGG GTTGGACTAA AGAAGTCAAC TCCACTCCTT
    GCTTATTTGC GGAACAATTT GCTGATGTTG GCCATGTTAG CTTTTGAAGT AACCATTTAC
    CGGCATCAGG AGTATTACAG GTTGCGAAAC AATCTCACCA CACCTGCAAC AAAAACCATC
    TTTCATGACA TCACAAGACT TCACTTGGAT GATGGACTCA TAAATAGTGC CAAGTATTTT
    GTGAACTACT TTTTCTACAA GTTTGGATTG GAAACATGTT TCTTACTGTC GGTAAATGTA
    ATCGGTCAGA GGATGGACTT TTTTGCCATG GTTCATGCAC TTTGGCTTTT TGGCGTATTA
    TACCGACGCA GAAGGAAAGC GATTGCAGAA ACCTGGCACA GATATTGCTG TTTTCTTGCT
    TGCATTATGG CATTCCAGTA TTTTATATGT ATTGGCATCC CCCCTGCTGC ATGTAAAGAC
    TATCCTTGGA GACAACCTGG AGCCAATTTT AATTCTAACA TAATAAAGTG GTTATACTTT
    CCAGACTTTA TTGTGAGGCC TAGTCCTCTG TTTCTTGTCT ATGATTTTTT GCTTTTGCTT
    TGTGCGTCCC TTCAAAGAGA AGTGTTTGAG GAAGAAAATA AGGCAGCAGT TCGAATCATG
    GCTGGTGACA ATGTAGAAAT TTGCATGAAC CTTGATGCTG CATCTTTTAG CCAACATAAT
    CCTGTACCTG ATTTCATATA CTGCAGATCT TATTTGGACA TGGGTAAAGT TATAATGTTT
    AGTTACCTTT TCTGGTTTGT GCTCACCATC ATCTTCATCA CGGGAACTAC ACGGATCAGC
    ATATTCTGCA TGGGCTACTT GGTAGCTTGC TTCTATTTTC TATTATTTGG TGGAGATTTG
    CTGTTAAAAC CTATAAGAAT CATCCTACGC TATTGGGATT GGCTGATTGC TTACAATGTT
    TTTGTCATTA CAATGAAAAA TGTATTATCA ATAGGAGCAT GTGGCTACAT TGAATATTTA
    ATCAAAGACA GTTGTTGGTT AATTCAGGCA TTCAGTCTTT CTTGTACAGT GAAAGGTTAT
    TTTATCCCCC AAAACACACC TGACTGTCAG TTGCCAAGCG GAGAGGCTGG AATCATTTGG
    GACAGTATAT GTTTTGCTTT CCTTTTATTG CAAAGAAGAG TGTTTATGAG CTATTACTTC
    TTACATGTGG TTGCAGACAT CAAATCATCT CAGATTCTAG CATCAAGAGG TGCTGAACTG
    TTCCAAGCTA CAATTGTAAA AGCTGTAAAA GCAAGAATTG AAGAAGAAAA AAAATCTATG
    GACCAGCTCA AGAGACAGAT GGACCACATT AAAGCCAGGC AAGAAAAATA TAAAAAAGGT
    AAAGAAAAAA TGCTGAGTCT TACACAGGAT GGAGAAGGAC AAGAAGTTCA AAAACTAGAT
    GATGACGATG AAGAAGGAGA CAAAGCAGAC AAAGAGAAAG CCAAGGGCAA AAAAAAGCAG
    TGGTGGCGAC CTTGGGTTGA TCATGCTTCC ATGGTGAAAA GCGGTGATTA TTATTTGTTT
    GAGACGGACA GTGAAGAAGA AGATGAGGAT GACAAAAAAG AAGAAGAGCC TAGAAGAAAA
    TCTGCCTTCC AGTTTTTGTA CCAAGCATGG ATTACTGATC CAAAAACAGC CCTTAAGCAA
    AGAAGCATGG AAGCCAAAGA AGAAAAGGCA AGGGAAATGA GGCGAAAGGG CATTGCTGAA
    GGTGATATTA TTGTAGAATG TGGGGATAAA GAAGAAGAAA AAGAGCAAGG AAAAAAGAAA
    TCAGATGGCC CAGACAATAT CTTCAAAAGA ATATTTAACA TCCTGAAGTT TACTTGGGTC
    CTCTTCCTTG CTACAATCGA TAGTTTTACG GCTTGGCTAA ACTCCATTTC AAGAGAACAT
    ATTGATATCT CCAAAGTTCT AAGCATAGAA CGTTGCATGC TGACTAGAGA GATAAAAAAG
    GGCAATGTGC CTACTCGAGA AAGCATCCAC ATGTATTACC AAAATCAAAT AATGATGAGC
    CTTTCTAGAG AGTCTGGACT TGACAGAATT GATGACCAAC CTGGTTCATC GTCCAGTCAC
    CATAGCCATG ACAAGATGGC GAGTAAAAGG ATGGACAGTG CAGACTCAGC TGTATCTCAT
    GATAGCATAT CAAGTGAGCC AACTCAGTGC ACCATGGTCT ACTCACGGCA GGGCACAACA
    GAAACTATTG AGGAAGTGGA AGATGAAGAG GAGGAAGAAA GCAAAAGTCC TTTATCAAAG
    ATAGAAGCCG AGGAAGAAGA ATATGACATG GATTTGGAAG GGACAAGCTA TACTCCGGAC
    ACAGAATTTC CCCTGTACGC CACTACAGAG GGGGATCTTC CTCCTTCGTA CAGTAAAGCC
    GGCAGCTTCG AACAACTTAC ATTTGTATCT CAGGACTCGG CTGACAATTT AATGGTTCTA
    AGCCCAGATG AAAGTCGTAC TGATAAGCTA CATGATCATA TTCTTCCACC TCTTACACAT
    GAACTGACTG CTAGCGAGCT GCTTCTAAAC AAAATGTTTT ATGATGAAGA GTTAGAAGAC
    TCTGAAAAAT TTTATACTAC TCAACCACGC CCGCTGCAGC TTATTTATGC CTTGTATAAT
    ACATTGGTGG CCAGATCAGA AATGGTGTGC TATTTTGTGA TTATTCTTAA TCACATGATT
    TCAGCTTCTA TGATCACATT GGTACTGCCC ATCCTTATAT TCCTTTGGGC TATGCTTTCT
    GTCCCAAGGC CCAGTAAGAG ATTTTGGATG ACAGCTATTG TATATACAGA GGTGACAATT
    GTTATTAAAT ATTTTTTCCA ATTTGGATTC TTCCCATGGA ATAAAAATGT GGATTATACA
    AAGGACAAAC CTTACTTCCC ACCTAACATA ATGGGCATTG AGAAGAAAGA AGGTTATGTG
    CACTATGACC TGGTGCAACT GCTTGCTCTG TTTTTCCATA GATCTATTTT AAAGTGCCAC
    GGGCTTTGGG ATGAAGATGA TGTCACTGAA AAAGCCACAG ATAAAGACGA CACGGATGGA
    GAGATATCAG AGGCATCAGG AAGAAGGGAT TCCGTTGGCT CATTGAAATC TGTCAATCTA
    GCTGCGTCTG TGGAGTCAGT CCATGTGCAC TTCCCTGAGG AAAAGACCGC TATCAGGAGA
    AAAAGCACAA GCAGTGGATC GCAAATTTCC CGAAGGTCAA GTTATTCCTC GCACAGGTCT
    AAAAGAGGCA GCACCAGTAC TCGGAACAGC AGTCAAAAAG GAAGCAGCAT TCCCAGTATT
    AAACTGAAAT CTAGAAAGGA ACTTATACTG GAAAAACTCA GAGAACAGCT CATTAAAGCA
    AAAGCATTTA CGATCAAGAC GATACTACAA ATCTATGTCC CGATCAGACA GTTTTTTTAC
    AATCTTATAC ATCCTGAATA CAGTGCTGTG ACTGATGTCT ATGTGCTGAT GTTCCTGGCA
    GACACTGTAG ATTTCATCAT TATTGTTTTT GGATTTTGGG CGTTTGGAAA ACACTCCGCT
    GCAGCAGATA TTACATCCTC TCTATCAGAA GACCAAGTCC CAGAAGCATT TTTGGTAATG
    GTGCTTATCC AGTTTGGAAC CATGGTTGTT GATCGTGCTT TGTATCTTCG CAAAACAGTC
    ATGGGAAAAG TTATTTTCCA AGTTATCCTT GTATTTGGAA TCCATTTTTG GATGTTCTTC
    ATCTTGCCAG GGGTAACAGA AAGGAAATTT AGTCAGAACA CTGTTGCCCA ACTGTGGTAT
    TTTGTCAAGT GCATTTATTT TGGATTGTCG GCATATCAGA TACGTTGTGG ATATCCAACT
    CGGGTGCTTG GTAACTTCCT TACTAAAAGC TACAATTATG TCAACCTGTT CCTTTTCCAA
    GGCTTTCGCC TCGTTCCATT TCTTACGGAG CTGCGAGCTG TTATGGACTG GGTATGGACT
    GACACCACTC TCAGTCTTTC CAGCTGGATC TGTGTAGAGG ACATTTATGC ACATATATTT
    ATACTAAAAT GCTGGCGGGA GTCAGAAAAG AGATATCCCC AGCCACGAGG CCAAAAGAAG
    AAAAAGGTTG TGAAATACGG AATGGGTGGA ATGATAATAG TTCTGCTGAT TTGTATCGTG
    TGGTTTCCTT TGCTCTTCAT GTCCTTGATT AAATCAGTTG CTGGGGTCAC TAACAAGCCC
    CTAGATGTGT CCATTACAAT AACCCTTGGA GGTTATCAGC CTATTTTTAC CATGAGTGCA
    CAACAAAATC AGCTGCAAGG CTTGAATGAC AATGAATTTA AGCATTTACA TAATATATAT
    AAAGGCAATA CACCTGCCAT GCAGTTTCTG GAATCCTATA TGCAGGAAGA CATCACCATA
    GCAGGACTGG AAGGCAATTC CAACTCTCTG TGGACCATCA GCCCTCCGAG CAGGACAATG
    ATGATAGAAA GACTTATCCA GGAACCTGAC TTCACAGCGG TCATTTCTTG GTCCATTCGA
    AGAAATGTGA GTCTTGGAGC TAAGGCAGAG GTAACAGGTG AAAAATATAC AATATCACTG
    GCAGGTCAAA CTCGGAGGGA ACTTGCGGAT ATGATGAATA GTTCATCTCC CAATAAGACA
    GTGGAACTGA TAAAGATATA CCCATTGTGT TTAAAAGCAC CAAGTGATGT TACTGCAAAA
    CCCATCCTTC AGCTTCTTAA GAATCAAGAG TTTGTGAATA TTAGCGTTTT ACTAAGAAAA
    GAAAACACAT CAGAAGGGGT TCGAGAATGG TGGGTAGTGA ATCAAAGCCA TAAAGAGTTC
    ATTAAGAAGG ATGGCCTGGA ACTATGGGTT TTCAGTGACA AAGTCAGTCC TCCAAGTCTT
    GGATTCTTGG CAGGCTATGG AATAATGGGA CTATATGCCT CTGTAGTTCT CGTAATAGGA
    AAGTTTGTGC GTGAGTTTTT CAGCGGAATT TCGCACTCCA TCATGTTTGA GGAGCTTCCC
    AACGTTGACC GTATACTGAA GCTGTGCACA GACATTTTCC TTGTGAGAGA GACTGGAGAG
    TTGGAACTGG AGGAAGACCT GTATGCCAAA TTAATATTTT TGTACCGCTC CCCAGAAACT
    ATGATCAAAT GGACAAGAGA GAAAGATAAA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012820819.2
    CDS632..8764
    Translation

    Target ORF information:

    RefSeq Version XM_012965365.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis piezo-type mechanosensitive ion channel component 2 (piezo2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012965365.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    ATGGCATCGG AAGTGGTGTG TGGGCTCATC TTCAGATTTC TTCTCCCACT ATGCCTGGTT 
    GTAGCGTGCA TGTTCCGGTA CAATGGAATA TCTTTTGTCT ACCTCATCTA CCTCTTGCTT
    ATACCTTTAT TTTCGGAGCC TACCAAAATA ACAATGCAAG GTCATACAGG ACGGTTATTA
    AAATCCTTAT GTGTTTCCAG TCTGTCATTT CTGCTGCTGC ACATCATCTT TCAAATTACC
    GTATACAGCC TCGAGGCCAG AAAACACATT GAACCACATT TTAACTGTTC AACATGGGAG
    AAAGCAATAC GACAAATTGG TTTTGAAAGT GTAAAAGGAG CAGATGCTGG TAATGGAATC
    CGCGTGTTTG CACCAGATAT CGGAATGTTT ATTGCAAGCT TGGCTACCCT GTTGGTTTGT
    AGAAAACTAG TTCAGAAAAC AGTTACTGAA GAGGACGCAC AATACAACCC CCAGTTTGTA
    AATGAAGAAA TGGCAGAAGA AGGAAAGGTG GACCTGGATG AAGCGTTAAT GTATGAGGAA
    GACTTTGATG GAGAAGAGGA TGAAATTGAG GCAGCTGAAG AAAGCACAAA ATTAAAAATT
    CTTCGCAGAA TTGCTTCATT TGCATCCGTA CTTAAAGAGT TTATTGGCAA CATTATAACC
    ACAGCTGGGA AAGTAGTTAT TACAATTTTA CTTGGTTTAA CAGGCATGAT GTTGCCGTCT
    TTGACCTCAG CTGTGTATTT CTTTGTATTT TTGGGGCTAT GCACATGGTG GTCCTGTTGC
    CGAGTATTTG ATCCGCTGAT ATTCAGCTGT CTATGTGTAT TAATGGCTAT ATTTAGTGCA
    GGGCACTTAA TTGGACTTTA TCTGTACCAG TTGCAGTTTT TTCACGAGAT TATTCCACCA
    AATGATTTCT ATGCCAGGTT GTTTGGTGTT ACTTCATTAG TTCAAACCGA CTGCTGTAAC
    ACTTGGAGAT TTTTTGCAAA CAACAGTCTA CCATGGAACC AGTATACCAA TCCTGTCATG
    CTTTTAGTAC TGTATTACAC GCTGGCAACT CTCATACGCC TTTGGCTACA GGAACCTATT
    AATGTAGAGG AAGAGAAGGA GGAAGACTGT AGCCCAGCAG CACTTACTGC AGAAAGAAGA
    CGTAGTTTGT GGTACTCTAG GCACTACACA ACGGATGAAA GACAACTTCT GTCCATGACT
    CAAGATGACT ACAAGCCATC TGATGTTTTA CTAGTAACTG TAAATGGGAC TCCCGTAGAT
    TACTGTTCAC TAAATTCATC ACTTCCGATA GAAAATGGTC CAACTAAAAT TGACATGTAT
    TCAACACCGC AATATAAATG GGACCATGAA GATGAAACTT CAGAAAAGAA AGAAGAGGAA
    GAGCAAGAAG AAGAACCCAA TGAAGAGGTT GCAGAAGAGA AAGAAAACAC TAAAGTTCAT
    GTAATGGTGG TGGCATTTCA GTTCATTATG AAGCAAAGCT ATATATGTGC ACTAATTTCA
    ATGATGGCCT GGAGCATCAC ATACCACAGC TGGTTGACTT TTGTTTTGTT AATCTGGTCT
    TGTGCTCTCT GGATGGTCCG AAATAGGAGA AAATATGCTA TGCTTAGTTC ACCTTTTATG
    GTTATGTATG GTAATTTACT TTTAACATTA CAATACATAT GGAGCATAGC CTTACAAAGT
    GATGAACTTC CAGAAGTTAT GGGTCTCCTT GAGAGAAAAA AGCCCGAAGA GCTTGTATCG
    AAGATCATTT TCACAGTTAC ATTTTGGCTT TTGCTCAGAC AACACCTTAC AGAGCAAAAG
    GCATTAAAGC AAAAAGAATT AGCATTATCT GAAATAAAAG TTGGAAGTGA AGACAAAGAA
    GGTGAAGTGC AAGAAGAAGA AGAAGAAGTG GAGGATGAAA ATGATATCAT GAAGGTCCTC
    GGAAAGCTAG TGCAGGCTAT GTTTGTCAAG TACTGGATTT ATGTCTGTGG CGGCATGTTT
    TTTTTTGTCA GCTTTGAAGG AAAAATAGTT ATGTACAAAA TTATCTACAT GATGCTCTTC
    CTATTCTGTG TAGCTCTGTA TCAGGTGCAC TATGAATGGT GGAGACGAAT TTTAAAATAT
    TTTTGGATGT CTGTGGTTGT TTACACCATG TTGGTGCTTG TTTTTATTTA CACTTACCAA
    TTTAAATCAT TTCCGGGCCT TTGGATGAAT ATGACAGGGC TGGATAAGGA AAAGTTAGCA
    GATCTTGGTT TAGAGACATT TAACTTGACT GACCTATTTA CTCGTATATT TATCCCAACC
    TCATTTTTGT TAGTGTGCAT TTTGCACCTA CATTACTTCC ATGACCGTTT TCTCCAGCTC
    ACTGACCTAA AGGCTGTAAC CAGCAAGCAG GACAGCACAA TTTACAGATT GGTGCATCCA
    GAAGGAAGTT TGCCTGATAT AACGATGATG AACTTAACAA CAGAATCTGA AATGCTTGAG
    GAAAAGCGGT TGGAAATTTA TGACGAAAAA AAAACTCATG ACCAAGACTC AGATGATGAA
    TGGGATAAAG GGGATAGCAA AGAACCAGGT CATTCTGATG ATGAAGCTGA GGATGAGGAA
    TCAGAAGAAG AAGAAACAAC AACAGAGTCC AGGAACAAGT GGCATCTAGT GATAGATCGC
    TTAACGGTTC TTTTCCTAAA GTTTCTGGAA TATTTTCACA AGTTTCAGGT CTTTGTTTGG
    TGGCTTCTGG AGCTTCACAT GATAAAGATT GTTTCTTCAT ACATCATTTG GCTTACTATA
    AAGGAGGTTT CACTGCTCAA TTATGTATTT TTTATTGCCT GGGCCTTCGC TTTGCCCTAC
    TCACAATTCC GGCCATTGGC ATCAAGTGTT TGCACAGTCT GGACATGTGT CATCATCATA
    TGTAAAATGT TGTACCAGCT AGCCTCCATT GACCCTGCAA CCTTTTCCAG CAACTGTTTA
    ATGCCACAGC TAAACGAAAC AACTGCACGT AACCAAACTG AGCTTAATTC TTCTGTGCTT
    TACAATTCCT CTGTTGATCC TTCTAATTGG GTTGGACTAA AGAAGTCAAC TCCACTCCTT
    GCTTATTTGC GGAACAATTT GCTGATGTTG GCCATGTTAG CTTTTGAAGT AACCATTTAC
    CGGCATCAGG AGTATTACAG GTTGCGAAAC AATCTCACCA CACCTGCAAC AAAAACCATC
    TTTCATGACA TCACAAGACT TCACTTGGAT GATGGACTCA TAAATAGTGC CAAGTATTTT
    GTGAACTACT TTTTCTACAA GTTTGGATTG GAAACATGTT TCTTACTGTC GGTAAATGTA
    ATCGGTCAGA GGATGGACTT TTTTGCCATG GTTCATGCAC TTTGGCTTTT TGGCGTATTA
    TACCGACGCA GAAGGAAAGC GATTGCAGAA ACCTGGCACA GATATTGCTG TTTTCTTGCT
    TGCATTATGG CATTCCAGTA TTTTATATGT ATTGGCATCC CCCCTGCTGC ATGTAAAGAC
    TATCCTTGGA GACAACCTGG AGCCAATTTT AATTCTAACA TAATAAAGTG GTTATACTTT
    CCAGACTTTA TTGTGAGGCC TAGTCCTCTG TTTCTTGTCT ATGATTTTTT GCTTTTGCTT
    TGTGCGTCCC TTCAAAGAGA AGTGTTTGAG GAAGAAAATA AGGCAGCAGT TCGAATCATG
    GCTGGTGACA ATGTAGAAAT TTGCATGAAC CTTGATGCTG CATCTTTTAG CCAACATAAT
    CCTGTACCTG ATTTCATATA CTGCAGATCT TATTTGGACA TGGGTAAAGT TATAATGTTT
    AGTTACCTTT TCTGGTTTGT GCTCACCATC ATCTTCATCA CGGGAACTAC ACGGATCAGC
    ATATTCTGCA TGGGCTACTT GGTAGCTTGC TTCTATTTTC TATTATTTGG TGGAGATTTG
    CTGTTAAAAC CTATAAGAAT CATCCTACGC TATTGGGATT GGCTGATTGC TTACAATGTT
    TTTGTCATTA CAATGAAAAA TGTATTATCA ATAGGAGCAT GTGGCTACAT TGAATATTTA
    ATCAAAGACA GTTGTTGGTT AATTCAGGCA TTCAGTCTTT CTTGTACAGT GAAAGGTTAT
    TTTATCCCCC AAAACACACC TGACTGTCAG TTGCCAAGCG GAGAGGCTGG AATCATTTGG
    GACAGTATAT GTTTTGCTTT CCTTTTATTG CAAAGAAGAG TGTTTATGAG CTATTACTTC
    TTACATGTGG TTGCAGACAT CAAATCATCT CAGATTCTAG CATCAAGAGG TGCTGAACTG
    TTCCAAGCTA CAATTGTAAA AGCTGTAAAA GCAAGAATTG AAGAAGAAAA AAAATCTATG
    GACCAGCTCA AGAGACAGAT GGACCACATT AAAGCCAGGC AAGAAAAATA TAAAAAAGGT
    AAAGAAAAAA TGCTGAGTCT TACACAGGAT GGAGAAGGAC AAGAAGTTCA AAAACTAGAT
    GATGACGATG AAGAAGGAGA CAAAGCAGAC AAAGAGAAAG CCAAGGGCAA AAAAAAGCAG
    TGGTGGCGAC CTTGGGTTGA TCATGCTTCC ATGGTGAAAA GCGGTGATTA TTATTTGTTT
    GAGACGGACA GTGAAGAAGA AGATGAGGAT GACAAAAAAG AAGAAGAGCC TAGAAGAAAA
    TCTGCCTTCC AGTTTTTGTA CCAAGCATGG ATTACTGATC CAAAAACAGC CCTTAAGCAA
    AGAAGCATGG AAGCCAAAGA AGAAAAGGCA AGGGAAATGA GGCGAAAGGG CATTGCTGAA
    GGTGATATTA TTGTAGAATG TGGGGATAAA GAAGAAGAAA AAGAGCAAGG AAAAAAGAAA
    TCAGATGGCC CAGACAATAT CTTCAAAAGA ATATTTAACA TCCTGAAGTT TACTTGGGTC
    CTCTTCCTTG CTACAATCGA TAGTTTTACG GCTTGGCTAA ACTCCATTTC AAGAGAACAT
    ATTGATATCT CCAAAGTTCT AAGCATAGAA CGTTGCATGC TGACTAGAGA GATAAAAAAG
    GGCAATGTGC CTACTCGAGA AAGCATCCAC ATGTATTACC AAAATCAAAT AATGATGAGC
    CTTTCTAGAG AGTCTGGACT TGACAGAATT GATGACCAAC CTGGTTCATC GTCCAGTCAC
    CATAGCCATG ACAAGATGGC GAGTAAAAGG ATGGACAGTG CAGACTCAGC TGTATCTCAT
    GATAGCATAT CAAGTGAGCC AACTCAGTGC ACCATGGTCT ACTCACGGCA GGGCACAACA
    GAAACTATTG AGGAAGTGGA AGATGAAGAG GAGGAAGAAA GCAAAAGTCC TTTATCAAAG
    ATAGAAGCCG AGGAAGAAGA ATATGACATG GATTTGGAAG GGACAAGCTA TACTCCGGAC
    ACAGAATTTC CCCTGTACGC CACTACAGAG GGGGATCTTC CTCCTTCGTA CAGTAAAGCC
    GGCAGCTTCG AACAACTTAC ATTTGTATCT CAGGACTCGG CTGACAATTT AATGGTTCTA
    AGCCCAGATG AAAGTCGTAC TGATAAGCTA CATGATCATA TTCTTCCACC TCTTACACAT
    GAACTGACTG CTAGCGAGCT GCTTCTAAAC AAAATGTTTT ATGATGAAGA GTTAGAAGAC
    TCTGAAAAAT TTTATACTAC TCAACCACGC CCGCTGCAGC TTATTTATGC CTTGTATAAT
    ACATTGGTGG CCAGATCAGA AATGGTGTGC TATTTTGTGA TTATTCTTAA TCACATGATT
    TCAGCTTCTA TGATCACATT GGTACTGCCC ATCCTTATAT TCCTTTGGGC TATGCTTTCT
    GTCCCAAGGC CCAGTAAGAG ATTTTGGATG ACAGCTATTG TATATACAGA GGTGACAATT
    GTTATTAAAT ATTTTTTCCA ATTTGGATTC TTCCCATGGA ATAAAAATGT GGATTATACA
    AAGGACAAAC CTTACTTCCC ACCTAACATA ATGGGCATTG AGAAGAAAGA AGGTTATGTG
    CACTATGACC TGGTGCAACT GCTTGCTCTG TTTTTCCATA GATCTATTTT AAAGTGCCAC
    GGGCTTTGGG ATGAAGATGA TGTCACTGAA AAAGCCACAG ATAAAGACGA CACGGATGGA
    GAGATATCAG AGGCATCAGG AAGAAGGGAT TCCGTTGGCT CATTGAAATC TGTCAATCTA
    GCTGCGTCTG TGGAGTCAGT CCATGTGCAC TTCCCTGAGG AAAAGACCGC TATCAGGAGA
    AAAAGCACAA GCAGTGGATC GCAAATTTCC CGAAGGTCAA GTTATTCCTC GCACAGGTCT
    AAAAGAGGCA GCACCAGTAC TCGGAACAGC AGTCAAAAAG GAAGCAGCAT TCCCAGTATT
    AAACTGAAAT CTAGAAAGGA ACTTATACTG GAAAAACTCA GAGAACAGCT CATTAAAGCA
    AAAGCATTTA CGATCAAGAC GATACTACAA ATCTATGTCC CGATCAGACA GTTTTTTTAC
    AATCTTATAC ATCCTGAATA CAGTGCTGTG ACTGATGTCT ATGTGCTGAT GTTCCTGGCA
    GACACTGTAG ATTTCATCAT TATTGTTTTT GGATTTTGGG CGTTTGGAAA ACACTCCGCT
    GCAGCAGATA TTACATCCTC TCTATCAGAA GACCAAGTCC CAGAAGCATT TTTGGTAATG
    GTGCTTATCC AGTTTGGAAC CATGGTTGTT GATCGTGCTT TGTATCTTCG CAAAACAGTC
    ATGGGAAAAG TTATTTTCCA AGTTATCCTT GTATTTGGAA TCCATTTTTG GATGTTCTTC
    ATCTTGCCAG GGGTAACAGA AAGGAAATTT AGTCAGAACA CTGTTGCCCA ACTGTGGTAT
    TTTGTCAAGT GCATTTATTT TGGATTGTCG GCATATCAGA TACGTTGTGG ATATCCAACT
    CGGGTGCTTG GTAACTTCCT TACTAAAAGC TACAATTATG TCAACCTGTT CCTTTTCCAA
    GGCTTTCGCC TCGTTCCATT TCTTACGGAG CTGCGAGCTG TTATGGACTG GGTATGGACT
    GACACCACTC TCAGTCTTTC CAGCTGGATC TGTGTAGAGG ACATTTATGC ACATATATTT
    ATACTAAAAT GCTGGCGGGA GTCAGAAAAG AGATATCCCC AGCCACGAGG CCAAAAGAAG
    AAAAAGGTTG TGAAATACGG AATGGGTGGA ATGATAATAG TTCTGCTGAT TTGTATCGTG
    TGGTTTCCTT TGCTCTTCAT GTCCTTGATT AAATCAGTTG CTGGGGTCAC TAACAAGCCC
    CTAGATGTGT CCATTACAAT AACCCTTGGA GGTTATCAGC CTATTTTTAC CATGAGTGCA
    CAACAAAATC AGCTGCAAGG CTTGAATGAC AATGAATTTA AGCATTTACA TAATATATAT
    AAAGGCAATA CACCTGCCAT GCAGTTTCTG GAATCCTATA TGCAGGAAGA CATCACCATA
    GCAGGACTGG AAGGCAATTC CAACTCTCTG TGGACCATCA GCCCTCCGAG CAGGACAATG
    ATGATAGAAA GACTTATCCA GGAACCTGAC TTCACAGCGG TCATTTCTTG GTCCATTCGA
    AGAAATGTGA GTCTTGGAGC TAAGGCAGAG GTAACAGGTG AAAAATATAC AATATCACTG
    GCAGGTCAAA CTCGGAGGGA ACTTGCGGAT ATGATGAATA GTTCATCTCC CAATAAGACA
    GTGGAACTGA TAAAGATATA CCCATTGTGT TTAAAAGCAC CAAGTGATGT TACTGCAAAA
    CCCATCCTTC AGCTTCTTAA GAATCAAGAG TTTGTGAATA TTAGCGTTTT ACTAAGAAAA
    GAAAACACAT CAGAAGGGGT TCGAGAATGG TGGGTAGTGA ATCAAAGCCA TAAAGAGTTC
    ATTAAGAAGG ATGGCCTGGA ACTATGGGTT TTCAGTGACA AAGTCAGTCC TCCAAGTCTT
    GGATTCTTGG CAGGCTATGG AATAATGGGA CTATATGCCT CTGTAGTTCT CGTAATAGGA
    AAGTTTGTGC GTGAGTTTTT CAGCGGAATT TCGCACTCCA TCATGTTTGA GGAGCTTCCC
    AACGTTGACC GTATACTGAA GCTGTGCACA GACATTTTCC TTGTGAGAGA GACTGGAGAG
    TTGGAACTGG AGGAAGACCT GTATGCCAAA TTAATATTTT TGTACCGCTC CCCAGAAACT
    ATGATCAAAT GGACAAGAGA GAAAGATAAA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.